LOCUS       DAOFQD010000001        92018 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.001, whole genome shotgun sequence.
ACCESSION   DAOFQD010000001 DAOFQD010000000
VERSION     DAOFQD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 92018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.001"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1382
                     /locus_tag="QBF46_000001"
     CDS             <1..1382
                     /locus_tag="QBF46_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019439188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6672247.1"
                     /translation="LLLAGFVTIFDLFVVNVAIPSMQADLGANFAQIGFIVAGYELAF
                     GVLLITGGRLGDLFGRRRLFVTGMAGFTLASALCGLAPSAEFLIGARVLQGLAAALLF
                     PQVYASIRVNFDGDDSRRAFGLLGMTLGLAAIAGQVLGGWLVHADLFGLGWRSIFLIN
                     VPIGLFAIAAARTIPESCAPQRPALDWMGVALVSAGLTLLLVPLIEGPGQGWPAWSLL
                     SLGVAVLLLALFHRQQEQRRMAGGLPLVDMRLLAQRRFALGAVLVLLVYSTSSSFFLC
                     FALLVQTGLGLDPFMAGSIFAPCSVGFVLASLAAPRLVARWGTRAIVAGAMVYAVSIG
                     LLIAQVWMAGAELVPARLIPVLVVVGAGQGFIMTPLLNLVLGFVDEAQAGMASGVIST
                     VQQVGAALGVAVVGILFAAALAADESVAAQAGVYASAFVAGMLYNLGAALLVCVLLLM
                     LARTQRLAG"
     gene            1397..2341
                     /locus_tag="QBF46_000002"
     CDS             1397..2341
                     /locus_tag="QBF46_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6672248.1"
                     /translation="MELRHLRCFLAVAEELHFARAAERLHIEQSPLSRTIKELEEDLG
                     EQLFIRTSRSTQLTRAGKLFLEHVPRIFTALQQARDSVQAAANGFHGQLRIALSDGIT
                     PSRLPALLALCRQEEPEVEIRLFEVPLSQQIKGLHDDLYDVGFAQADEVGDGVVAQAV
                     WSDPLMVAVPARHPLLRHKRIPLEEVLRHPLVLCDPQACEGHARQVERVLRRADTEPL
                     IAERVSSCDLMMALVSAGFALGITGAPHIAASRESGVVARPLAGRAPMLTTYVLHREG
                     ESSDVLERFIERVLAIESPEGYRSTSPVEPDSLEDNDP"
     gene            2344..2610
                     /locus_tag="QBF46_000003"
     CDS             2344..2610
                     /locus_tag="QBF46_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HDQ6672249.1"
                     /translation="MKLITPFLLVAALTACGPSEPPKQQANVPTVEELAAAPERLKEL
                     RRQCKTERPTMGDVLCNRVAEATNKRFFGDGKVPYTPSETPPKF"
     gene            2846..4852
                     /locus_tag="QBF46_000004"
     CDS             2846..4852
                     /locus_tag="QBF46_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HDQ6672250.1"
                     /translation="MQAQGVLFGQIAAVFGIVIAGVWGATQWTAAALGYQLRLGSPWF
                     DLYCTPVYYPWKLFEWWFFFDAYAPQVFDTGGMIAAGSGLLAVAVAIAMSVWRSRQSR
                     LVTTYGSARWANAADIRKAGLTQPAGVFLGLHAGQYLRHEGPEHVLTFAPTRSGKGVG
                     LVVPTLLSWPASAVIHDIKGENWQITAGWRSRFSHCLLFNPTDAKSAAYNPLLEVRRG
                     AHEVRDVQNIADILVDPEGALEKRNHWEKTSHALLVGAILHVLYAGEDKTLRGVANFL
                     SDPASPFELTLHRMMTTKHLGDAPHPIVASAAREVLNKSDNERSGVLSTAMSFLGLYR
                     DPTVAEVTSRCDWRIADLIASEYPVSLYLVVPPSDISRTKPLIRLILNQIGRRLTESL
                     DGSDGIERRHKLLLMLDEFPALGRLDFFETALAFMAGYGIRSFLIAQSLNQIDKAYGQ
                     NHSILDNCHVRVTFATNDERTAKRISETLGTATELRAQRNYAGHRLAPWLGHLMVSRQ
                     ETARPLLTPGEVMQLPTDEAVVMVSSVAPIKAKKLRYFADANFKQRVLPPPAVAAGRY
                     ADVPPVRPDDWSDLAIPAVPAAPATAFADDLETLGSTDDGGPRRQPELSEAIAYAPEM
                     DATTSDLSLLDDDDMPQLLPGQLDPALQRTARLASLDPNDGIDL"
     gene            4849..5337
                     /locus_tag="QBF46_000005"
     CDS             4849..5337
                     /locus_tag="QBF46_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDQ6672251.1"
                     /translation="MSQYRLNLFIQHEHAKRLDELAAKKGVSKSSIVAAALASWLSPD
                     AGDQREAAIAKRLDRLSRQAERLERDQNIQIETLALFIRYFLTVSTPVPEAHQDAARA
                     QGKARFEQFVEQLGRHLLRGRSLVRDVVEELHSQDREFGMRMDDAAAMVAAHERTAER
                     TP"
     gene            5334..6404
                     /gene="trbB"
                     /locus_tag="QBF46_000006"
     CDS             5334..6404
                     /gene="trbB"
                     /locus_tag="QBF46_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HDQ6672252.1"
                     /translation="MSAVPQIPPEPRSSAAASQDRRIQMLRTAMGPVIAAALEDPDVV
                     EVMLNPDRTLWVDRLSSGRAPLGVELPEADGERIIRLVAAHVGAEVHRGQPLLTAELP
                     ETGERFEGILPPAAPGPAFALRKRAVSIIGLDRYVADGILSAGQAEFLRHAVRERQNI
                     LIAGGTSTGKTTLANALLAEIAATGDRVLVLEDTIELQCAARDHVPLRTHAGVVSMQE
                     LVRATMRLRPDRVIVGEVRGGEALDLVKVWGTGHPGGIATIHAGSALGALLRLEQLIL
                     EVAVNPPRALIAEAVNVVIHIAGRGRKRHVETISRVVGFDGAGYRLADALEAPLPELP
                     PVPLAAAAAAPSLTTDQPGELP"
     gene            6401..6787
                     /locus_tag="QBF46_000007"
     CDS             6401..6787
                     /locus_tag="QBF46_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HDQ6672253.1"
                     /translation="MTHVDAFRLSVNPFSRLSSMARLRRLARPAGQGLLLAVLMLLLA
                     GTAQAAGSSMPWEGPLQSILESIQGPVARIVAVIIIIATGLALAFGDTSGGFRKLIQI
                     VFGLSIAFAASSFFLSFFSFSGGAVV"
     gene            6784..7053
                     /locus_tag="QBF46_000008"
     CDS             6784..7053
                     /locus_tag="QBF46_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HDQ6672254.1"
                     /translation="MSTATDLPGFEVPLHRSLTEPILLGGAPRTVAIANGTLAAAVGL
                     GLQLWIPGVVLWIAGHSLAVWGARVDPQFMQVFARHIKHKPLLDA"
     gene            7066..9516
                     /locus_tag="QBF46_000009"
     CDS             7066..9516
                     /locus_tag="QBF46_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HDQ6672255.1"
                     /translation="MLNLAEYRQRPALLADWLPWAGLIAPGVVLNKDGSFQRTARFRG
                     PDLDSATQGELIATSARLNNALRRLGSGWALFIEAERRAAAGYPCSEFPEPLSWLVEE
                     ERRAAFEESGHHYESAYHLTLAYLPPEESRARAAKLLYEHAPGDGVDWRGRLDAFVAE
                     TDRVFDLLDGVMPEIAWLDDAQTLTYLHATVSTRRYRLAVPEVPFHLDALLADSALVG
                     GLAPMLGDQHLRVVSVRGFPTSTWPGILDDLNRLGFGYRWSTRFLCLDKAEAEKELGR
                     LRRQWFAKRKNVVALLRETVFQQESPLVDTDANNKAADADAALQELGSDQVSFGYLTA
                     TVTVMDTDAAVADEKLRMVERVIQGRGFVTIPETLNAVDAWLSSIPGNAYANVRQPIV
                     STLNLAHMMPLSAVWAGPEKNAHLDGPPLIVTRTDGATPFRLVTHIGDVGHTLVAGPT
                     GMGKSVLLATLAMQFRRYPGSRIFAFDMGRSMRATILGLGGEHYDLGNDGAIAFQPLA
                     RIDHEGYRTWATEWVEGRLLHEGVAIGPDEKAAIWSALGSLAGAPVEQRTLTGLSVLL
                     QSNALRQALAPYVLGGAHGKLLDADADRLGSGSVQGFEMEELMHSKAAVLAVLGYLFA
                     RFDERFDGAPTLLILDEAWLFLDDPVFAARIRQWLKTLRKKNVSVIFATQSLADIKDS
                     SIAPAVIESCASRIFLPNPQATEPQIRTIYEGFGLNSRQIEIVATAQPKRDYYYQSRL
                     GNRLFDLDLGPATLAFAGASTPQDQRDMDRVLLEAGTPGFAGAWLRHRGLDWAADLLP
                     SSPAAASFLASQRPEVSP"
     gene            9513..10256
                     /gene="trbJ"
                     /locus_tag="QBF46_000010"
     CDS             9513..10256
                     /gene="trbJ"
                     /locus_tag="QBF46_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDQ6672256.1"
                     /translation="MKTQPRLLSVSLAAVLSVSLLASQPASALTVFDPSNFVQNTLTA
                     VRTLEQINNQINQLQNEAQMLMNQARNLANLDFNIVNRLRSTLATTERLIAEARGLAY
                     DVQSMDATFARLYPEQYAATVSGDQMLRDAQERWKNTLNGLHTAMRMQAQVSQNLAQD
                     ESALADLVSQSQSATGALQAMQATNQLLALQAKQSIQAQQLQITQERAAALELARQAA
                     AVERGREVTRRFLGDGTPYTPLRVDFYGN"
     gene            10269..10583
                     /locus_tag="QBF46_000011"
     CDS             10269..10583
                     /locus_tag="QBF46_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010280362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672257.1"
                     /translation="MRCAFLLLPVLLVACSQQPTDDLAAALAADPVRLKALRAQCAAD
                     RTTAGEYACLVAAEAFRRRFFSGETGPDEFRTLADLPPIPPSFDTPASDQDAMPFERD
                     VP"
     gene            10580..11959
                     /gene="trbL"
                     /locus_tag="QBF46_000012"
     CDS             10580..11959
                     /gene="trbL"
                     /locus_tag="QBF46_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HDQ6672258.1"
                     /translation="MNDVTVIDRFLATFSRYIDSGFGLLQGEVAFLTATLIVIDMTIA
                     GLYWAMSHATGQGDDVIAKLLRKVLYVGAFAYIINNFNWLASIVFRSFAGLGLTASGS
                     TMSMGEFLQPGRLAKTGIDAAAPILEQISDMAGFPEVFVNMTPIVVMFLAWAVVILCF
                     FVLAIQLFITLIEFKLTTLAGFVLVPFALWNKTSFLAEKVLGNVVSSGIKVLVLAVIV
                     GIGSGLFAEFQVHPAEPSIDHAVVIMLASLTLLALGIFGPGIATGLVSGAPQLGAGAM
                     AGAAIGAAGAAVAVGAAATGVGGAVMAGARMAPAAAKLAGAGARAATSAAGSAKSAFQ
                     AGSAAAGGGAKGAMAGLGSVAKGGAQAAGQRAGAGIKAAAAKAAAPFKAGWQGSGTDG
                     GFGGGAASSGQTAAGDANAQKQEQPGWAKRLQRRQQLTQATTTAAHTLRGGDGGGSGQ
                     GPSLRDSDS"
     gene            11977..12681
                     /locus_tag="QBF46_000013"
     CDS             11977..12681
                     /locus_tag="QBF46_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HDQ6672259.1"
                     /translation="MRFKRPQVRYADTPQPATPYQAAAQVWDERIGSARVQAKNWRLM
                     AFGCLVLALLMAGGLVWRSAQSIVTPYVIEVDQSGQVRTVGEAATPYRPTDAQTAHHI
                     ARFVTLVRSLSIDPIVVRQNWLDAYDYTTDRGAAVLNDYARTNDPFARIGKESVTVQI
                     TSVVRASDASFNVRWTERRYVNGAAAGLERWTAVVSIVQQTPRTEERLRRNPLGIYVN
                     GLSWSRELDSSEGVKP"
     gene            12678..13679
                     /gene="trbG"
                     /locus_tag="QBF46_000014"
     CDS             12678..13679
                     /gene="trbG"
                     /locus_tag="QBF46_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HDQ6672260.1"
                     /translation="MSVPFRFYALPLILLGLAGCATQGKPPPVISLDEPVQAQPLPEP
                     PTPIEVVAVPEPLALPAQLKPLPDVDAAPTTPESADEKVRVSRANAEARIAPTREGYV
                     NAIQVWPFTDGALYQVYASVGRVTVIALQPGEELVTVAAGDTVRWIVGDTSSGSGAEL
                     RVNVLVKPIRSSLKTNLVITTSRRTYLLELTSTDKAWMASVSWDYPKDRMLALQRQAQ
                     AANAAAPIDAGLSLENIRFRYAISGSNPPWKPLRAFDDGEKVYIQFPAGIAQGELPPL
                     FVIGAQGDGQLVNYRFRSPYYIVDRLFGAAELRLGADKGDVVRIERTDGVVSRSGRN"
     gene            13682..14959
                     /locus_tag="QBF46_000015"
     CDS             13682..14959
                     /locus_tag="QBF46_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HDQ6672261.1"
                     /translation="MSQEDTPDLAAPQVDKVAPEAVALRAQPRPVTRLNRRTLAILAG
                     GLSVGVMGALIWSLQPQQRGAGESTELYNVDRVSKSEGLDALPTDYSKLPALPLDVPE
                     LGPPLPGDLGPAIVNSQQPVAATYAAPGYDPNDALRKEEEAAAASSVFFRTGSPQAAP
                     VAQSQVAAAPGFAANAAFDPLAAGPASTAAQPADPTAVQNRQDQKEAFLAGGSTETRN
                     SGNLQMPTSPYQVMAGTVIAGALVTGIKSDLPGDVIATVTEPIYDTATGKFLLIPQGS
                     RILGKYNSQVSYGQSRVQVVWNRITLPDTSSLTLDNLVGTDPAGYAGLEDDVDWHWDR
                     IFAGAVLTTLLGVGAELAAPENRQDGDRVIIAGRDSLQDTVNQVGQEVTRRNLNIQPT
                     LTSRPGLPVRIIVNRDLVLRPYQPLFFNKGTSR"
     gene            14956..15174
                     /locus_tag="QBF46_000016"
     CDS             14956..15174
                     /locus_tag="QBF46_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2274 domain-containing protein"
                     /protein_id="HDQ6672262.1"
                     /translation="MNTTKKLRLGPLPRHEVTKLTFSCPASLKADLDRYAALHAQAYG
                     EAVDATTLIPHMLEAFMAGDRGFRKGDG"
     gene            15332..16582
                     /locus_tag="QBF46_000017"
     CDS             15332..16582
                     /locus_tag="QBF46_000017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672263.1"
                     /translation="MLEVDKKREATASIRGYFYQLDAALLEILNADLSDEIVIEGIED
                     FDRYTDESVIYGQVKYYAEQNLTDSVLRAPLHKLFVHFHGLEEAQRAGRKYLLYGHFS
                     EVKIDIGELSVERFKSVMEYRKEVKAADGTKSYEKKSLLDGMAAPDRLIEAFCKSFSI
                     QISKEFSEHRRLVIEAIRKNQSVSGVEAEGFHYPMAFDYIATLATKKDHNDRKVTRRD
                     LQELLKGAQAIHNRWLLREKDASEYAKHIKRLYFSLTNGAGIARVFIIECDGATDGSV
                     ICDQLRAIGNNWSSAKKRRIQNSERYAPFILLRGADETLILQVKNELFDTGTVFVDGY
                     PYRGSPFRNDHVQSPQTEVHQIEIRFVEDVDQLLEALNGVGRKLCHIYDFFLERPAAM
                     AMPGPKSRMYSIPVSSISTINKII"
     gene            16592..18331
                     /locus_tag="QBF46_000018"
     CDS             16592..18331
                     /locus_tag="QBF46_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDQ6672264.1"
                     /translation="MTEEVANAEVVAVYPNKVRVAVDDLLNFQAAGESLRVGSFLKVS
                     DNDNVSLICIIESFSIEMKEVKGDGISDHKRVYMIDSYPLGTLKDGKFNRGGDELAIP
                     PKKVVPATKAEIAAIYADCYAAEDRFCFSSLTRQRDIVVPVHGDRFFNKHIAVVGASG
                     SGKSSSVTSVLQKAVSAKSGEYQGLNNSHILIFDLHSEYATAFPKANIITVDDLVLPY
                     WLLNEDEMEDMFLESGDNNNYNQESLLRSVVTIAKKISNPAVEKVNFDSPLRYDIQHV
                     ANAFRNLTRETKDAENSLHIKFKDGTTETFDDVEGQLEHYCKALREFAAPERSKINKG
                     PYADGSIDKFERRIRSKISNSRLDFIFGAKAKAATLEQVLEQFTGYKAGGESNVTIID
                     LSGIPFELLSITVSLISRLMFDFCYYARRVESKTEQPFLMVYEEAHKYAPKSGLARYK
                     ASLAAIERIAKEGRKYGISLLISSQRPSEISETIFSQCSNFLAMRLTNPDDQSYVRRL
                     LPDTLGNLTEGMPALEQGEALLIGDAVIMPCVVYVEKCHPEPSSSDVKYLTLWKAEWK
                     EIDFKKRADNWRG"
     gene            18805..19443
                     /gene="nimT"
                     /locus_tag="QBF46_000019"
                     /pseudo
     CDS             18805..19443
                     /gene="nimT"
                     /locus_tag="QBF46_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            19498..19845
                     /locus_tag="QBF46_000020"
     CDS             19498..19845
                     /locus_tag="QBF46_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDQ6672265.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(19867..20121)
                     /gene="yoaF"
                     /locus_tag="QBF46_000021"
     CDS             complement(19867..20121)
                     /gene="yoaF"
                     /locus_tag="QBF46_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDQ6672266.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            20304..21329
                     /gene="dgcP"
                     /locus_tag="QBF46_000022"
     CDS             20304..21329
                     /gene="dgcP"
                     /locus_tag="QBF46_000022"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDQ6672267.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            21362..21460
                     /locus_tag="QBF46_000023"
     CDS             21362..21460
                     /locus_tag="QBF46_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDQ6672268.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            21463..21537
                     /gene="yoaJ"
                     /locus_tag="QBF46_000024"
     CDS             21463..21537
                     /gene="yoaJ"
                     /locus_tag="QBF46_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDQ6672269.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(21589..21843)
                     /locus_tag="QBF46_000025"
     CDS             complement(21589..21843)
                     /locus_tag="QBF46_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDQ6672270.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKVNA"
     gene            complement(21991..22173)
                     /locus_tag="QBF46_000026"
     CDS             complement(21991..22173)
                     /locus_tag="QBF46_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDQ6672271.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(22177..22536)
                     /gene="yeaR"
                     /locus_tag="QBF46_000027"
     CDS             complement(22177..22536)
                     /gene="yeaR"
                     /locus_tag="QBF46_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDQ6672272.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(22709..23347)
                     /gene="leuE"
                     /locus_tag="QBF46_000028"
     CDS             complement(22709..23347)
                     /gene="leuE"
                     /locus_tag="QBF46_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDQ6672273.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(23474..24397)
                     /gene="dmlR"
                     /locus_tag="QBF46_000029"
     CDS             complement(23474..24397)
                     /gene="dmlR"
                     /locus_tag="QBF46_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDQ6672274.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            24500..25585
                     /gene="dmlA"
                     /locus_tag="QBF46_000030"
     CDS             24500..25585
                     /gene="dmlA"
                     /locus_tag="QBF46_000030"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDQ6672275.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQTERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            25836..27446
                     /gene="yeaV"
                     /locus_tag="QBF46_000031"
     CDS             25836..27446
                     /gene="yeaV"
                     /locus_tag="QBF46_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDQ6672276.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            27478..28602
                     /gene="yeaW"
                     /locus_tag="QBF46_000032"
     CDS             27478..28602
                     /gene="yeaW"
                     /locus_tag="QBF46_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDQ6672277.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            28658..29623
                     /gene="yeaX"
                     /locus_tag="QBF46_000033"
     CDS             28658..29623
                     /gene="yeaX"
                     /locus_tag="QBF46_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDQ6672278.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFAQIPSARKHLFIAGGIGITPFLSHMAEQQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(29677..30792)
                     /gene="rnd"
                     /locus_tag="QBF46_000034"
     CDS             complement(29677..30792)
                     /gene="rnd"
                     /locus_tag="QBF46_000034"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDQ6672279.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(30874..32559)
                     /gene="fadD"
                     /locus_tag="QBF46_000035"
     CDS             complement(30874..32559)
                     /gene="fadD"
                     /locus_tag="QBF46_000035"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDQ6672280.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(32764..33345)
                     /gene="yeaY"
                     /locus_tag="QBF46_000036"
     CDS             complement(32764..33345)
                     /gene="yeaY"
                     /locus_tag="QBF46_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDQ6672281.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(33385..34080)
                     /gene="tsaB"
                     /locus_tag="QBF46_000037"
     CDS             complement(33385..34080)
                     /gene="tsaB"
                     /locus_tag="QBF46_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDQ6672282.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(34138..36048)
                     /locus_tag="QBF46_000038"
     CDS             complement(34138..36048)
                     /locus_tag="QBF46_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDQ6672283.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            36180..36524
                     /locus_tag="QBF46_000039"
     CDS             36180..36524
                     /locus_tag="QBF46_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDQ6672284.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            36886..37245
                     /locus_tag="QBF46_000040"
     CDS             36886..37245
                     /locus_tag="QBF46_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDQ6672285.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(37365..37544)
                     /locus_tag="QBF46_000041"
     CDS             complement(37365..37544)
                     /locus_tag="QBF46_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDQ6672286.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            37618..38979
                     /gene="pabB"
                     /locus_tag="QBF46_000042"
     CDS             37618..38979
                     /gene="pabB"
                     /locus_tag="QBF46_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDQ6672287.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRLVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIFCSAGGGIVADSQEEAEYQETFDKVNRILKQ
                     LEK"
     gene            38983..39561
                     /locus_tag="QBF46_000043"
     CDS             38983..39561
                     /locus_tag="QBF46_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDQ6672288.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASAIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            39745..41109
                     /gene="sdaA"
                     /locus_tag="QBF46_000044"
     CDS             39745..41109
                     /gene="sdaA"
                     /locus_tag="QBF46_000044"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDQ6672289.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            41240..42838
                     /locus_tag="QBF46_000045"
     CDS             41240..42838
                     /locus_tag="QBF46_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDQ6672290.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(42842..44398)
                     /gene="yoaE"
                     /locus_tag="QBF46_000046"
     CDS             complement(42842..44398)
                     /gene="yoaE"
                     /locus_tag="QBF46_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDQ6672291.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            44861..45832
                     /gene="manX"
                     /locus_tag="QBF46_000047"
     CDS             44861..45832
                     /gene="manX"
                     /locus_tag="QBF46_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDQ6672292.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            45895..46695
                     /gene="manY"
                     /locus_tag="QBF46_000048"
     CDS             45895..46695
                     /gene="manY"
                     /locus_tag="QBF46_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDQ6672293.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            46708..47559
                     /gene="manZ"
                     /locus_tag="QBF46_000049"
     CDS             46708..47559
                     /gene="manZ"
                     /locus_tag="QBF46_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDQ6672294.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            47614..48072
                     /locus_tag="QBF46_000050"
     CDS             47614..48072
                     /locus_tag="QBF46_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDQ6672295.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKSMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            48501..49067
                     /gene="mntP"
                     /locus_tag="QBF46_000051"
     CDS             48501..49067
                     /gene="mntP"
                     /locus_tag="QBF46_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDQ6672296.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(49064..49873)
                     /gene="rlmA"
                     /locus_tag="QBF46_000052"
     CDS             complement(49064..49873)
                     /gene="rlmA"
                     /locus_tag="QBF46_000052"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDQ6672297.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(50039..50248)
                     /gene="cspE"
                     /locus_tag="QBF46_000053"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(50039..50248)
                     /gene="cspE"
                     /locus_tag="QBF46_000053"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDQ6672298.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(50261..50404)
                     /locus_tag="QBF46_000054"
     CDS             complement(50261..50404)
                     /locus_tag="QBF46_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDQ6672299.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(51073..51360)
                     /locus_tag="QBF46_000055"
     CDS             complement(51073..51360)
                     /locus_tag="QBF46_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDQ6672300.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(51435..51578)
                     /gene="mgrB"
                     /locus_tag="QBF46_000056"
     CDS             complement(51435..51578)
                     /gene="mgrB"
                     /locus_tag="QBF46_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDQ6672301.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            51737..51976
                     /locus_tag="QBF46_000057"
     CDS             51737..51976
                     /locus_tag="QBF46_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672302.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(52119..52910)
                     /gene="kdgR"
                     /locus_tag="QBF46_000058"
     CDS             complement(52119..52910)
                     /gene="kdgR"
                     /locus_tag="QBF46_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDQ6672303.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            53087..54460
                     /locus_tag="QBF46_000059"
     CDS             53087..54460
                     /locus_tag="QBF46_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6672304.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(54506..55387)
                     /gene="htpX"
                     /locus_tag="QBF46_000060"
     CDS             complement(54506..55387)
                     /gene="htpX"
                     /locus_tag="QBF46_000060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDQ6672305.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(55579..57627)
                     /gene="prc"
                     /locus_tag="QBF46_000061"
     CDS             complement(55579..57627)
                     /gene="prc"
                     /locus_tag="QBF46_000061"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDQ6672306.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(57647..58345)
                     /gene="proQ"
                     /locus_tag="QBF46_000062"
     CDS             complement(57647..58345)
                     /gene="proQ"
                     /locus_tag="QBF46_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDQ6672307.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(58442..58939)
                     /gene="msrC"
                     /locus_tag="QBF46_000063"
     CDS             complement(58442..58939)
                     /gene="msrC"
                     /locus_tag="QBF46_000063"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDQ6672308.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            59069..60352
                     /gene="yebS"
                     /locus_tag="QBF46_000064"
     CDS             59069..60352
                     /gene="yebS"
                     /locus_tag="QBF46_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDQ6672309.1"
                     /translation="MALNTPQITPTKKITVRTIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            60321..62954
                     /gene="yebT"
                     /locus_tag="QBF46_000065"
     CDS             60321..62954
                     /gene="yebT"
                     /locus_tag="QBF46_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDQ6672310.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGAEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            63034..64473
                     /gene="rsmF"
                     /locus_tag="QBF46_000066"
     CDS             63034..64473
                     /gene="rsmF"
                     /locus_tag="QBF46_000066"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDQ6672311.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGE
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPTYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            64591..64827
                     /locus_tag="QBF46_000067"
     CDS             64591..64827
                     /locus_tag="QBF46_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDQ6672312.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            64932..65123
                     /locus_tag="QBF46_000068"
     CDS             64932..65123
                     /locus_tag="QBF46_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDQ6672313.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLHSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(65124..65780)
                     /gene="pphA"
                     /locus_tag="QBF46_000069"
     CDS             complement(65124..65780)
                     /gene="pphA"
                     /locus_tag="QBF46_000069"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDQ6672314.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGN
                     WFIALADNQQKQAKTALGKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKVQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(66176..66517)
                     /locus_tag="QBF46_000070"
     CDS             complement(66176..66517)
                     /locus_tag="QBF46_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDQ6672315.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(66530..67402)
                     /gene="copD"
                     /locus_tag="QBF46_000071"
     CDS             complement(66530..67402)
                     /gene="copD"
                     /locus_tag="QBF46_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDQ6672316.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     ITTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(67406..67780)
                     /gene="yobA"
                     /locus_tag="QBF46_000072"
     CDS             complement(67406..67780)
                     /gene="yobA"
                     /locus_tag="QBF46_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDQ6672317.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLMHQYPAANAQVTAS
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            67919..68149
                     /gene="holE"
                     /locus_tag="QBF46_000073"
     CDS             67919..68149
                     /gene="holE"
                     /locus_tag="QBF46_000073"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDQ6672318.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            68251..68907
                     /locus_tag="QBF46_000074"
     CDS             68251..68907
                     /locus_tag="QBF46_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDQ6672319.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLVASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            68931..69593
                     /gene="exoX"
                     /locus_tag="QBF46_000075"
     CDS             68931..69593
                     /gene="exoX"
                     /locus_tag="QBF46_000075"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDQ6672320.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(69590..71650)
                     /gene="ptrB"
                     /locus_tag="QBF46_000076"
     CDS             complement(69590..71650)
                     /gene="ptrB"
                     /locus_tag="QBF46_000076"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDQ6672321.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(71859..72518)
                     /locus_tag="QBF46_000077"
     CDS             complement(71859..72518)
                     /locus_tag="QBF46_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDQ6672322.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(72845..73201)
                     /locus_tag="QBF46_000078"
     CDS             complement(72845..73201)
                     /locus_tag="QBF46_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDQ6672323.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(73268..73558)
                     /gene="yebG"
                     /locus_tag="QBF46_000079"
     CDS             complement(73268..73558)
                     /gene="yebG"
                     /locus_tag="QBF46_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDQ6672324.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            73692..74870
                     /gene="purT"
                     /locus_tag="QBF46_000080"
     CDS             73692..74870
                     /gene="purT"
                     /locus_tag="QBF46_000080"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDQ6672325.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHIINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(74926..75567)
                     /gene="kdgA"
                     /locus_tag="QBF46_000081"
                     /gene_synonym="eda"
     CDS             complement(74926..75567)
                     /gene="kdgA"
                     /locus_tag="QBF46_000081"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDQ6672326.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(75604..77415)
                     /gene="edd"
                     /locus_tag="QBF46_000082"
     CDS             complement(75604..77415)
                     /gene="edd"
                     /locus_tag="QBF46_000082"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDQ6672327.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLGRIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(77650..79125)
                     /gene="zwf"
                     /locus_tag="QBF46_000083"
     CDS             complement(77650..79125)
                     /gene="zwf"
                     /locus_tag="QBF46_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDQ6672328.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            79463..80332
                     /gene="hexR"
                     /locus_tag="QBF46_000084"
                     /gene_synonym="yebK"
     CDS             79463..80332
                     /gene="hexR"
                     /locus_tag="QBF46_000084"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDQ6672329.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            80460..81902
                     /gene="pyk"
                     /locus_tag="QBF46_000085"
     CDS             80460..81902
                     /gene="pyk"
                     /locus_tag="QBF46_000085"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDQ6672330.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(81962..82804)
                     /locus_tag="QBF46_000086"
     CDS             complement(81962..82804)
                     /locus_tag="QBF46_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDQ6672331.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIEP
                     DSAINITKRFAKNMKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
     gene            complement(82827..83666)
                     /locus_tag="QBF46_000087"
     CDS             complement(82827..83666)
                     /locus_tag="QBF46_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ6672332.1"
                     /translation="MNDIEKYGNRYHVLQSFPMPQLASSREIRILLPASYYESDISYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPRNTSFPLPGWDPAVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRQDVFSKVGVFSPALWFNDSAMLNFIQENNIVEDLTVYLDVGT
                     QETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
     gene            complement(83759..84556)
                     /locus_tag="QBF46_000088"
     CDS             complement(83759..84556)
                     /locus_tag="QBF46_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDQ6672333.1"
                     /translation="MIIDAHTHIGDFVKISMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHAKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSEKVQDYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PDVKFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQAIKICGSDKILFGTD
                     NPINGLDTYNDDDFYNKYFAELKSKLTQSEFEQLMFTNAKKLFKIKI"
     gene            complement(84578..85801)
                     /locus_tag="QBF46_000089"
     CDS             complement(84578..85801)
                     /locus_tag="QBF46_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDQ6672334.1"
                     /translation="MGGDLWGVIDKLDYLEDLGINGIYFCPIFTSASNHKYDTIDHFS
                     VDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSPIWLDVVRNGANSRYADWFW
                     IHKFPVYPDTPKSERDFKNFNYETFGNVIEMPKLNTENEECREYLLSIVRYWTQNFDI
                     DGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEGTPWLRGDQFDSLMNYPLT
                     YGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLLDSHDTARILSVCLNDKRK
                     VKLAYLFMLTQAGSPCIYHGSEIGIDGYKSMTLENNRKCMVWEENKQDLELRKFIRWL
                     ISLRKKHPQWCEASIQWKDVEHPTVIAYQRDNITFFLNNSEDTASFIYNGRSMEIPGF
                     SYEIEGLPTADLYDF"
     gene            complement(85853..86376)
                     /locus_tag="QBF46_000090"
                     /pseudo
     CDS             complement(85853..86376)
                     /locus_tag="QBF46_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504365.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            complement(86393..87130)
                     /locus_tag="QBF46_000091"
     CDS             complement(86393..87130)
                     /locus_tag="QBF46_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDQ6672335.1"
                     /translation="MASFKKGNNLFSSTFSGIEFTFEHYVTLFTDTPYMQWYLNTFIL
                     ATANMLISLVVVTMTAYVFSRYRFRGKRNTLMSILVLQMFPAFLSMTAIYILLSKANL
                     IDTYTGLLLVYVTGSLPFMTWLVKGYFDAIPTSLDEAAKIDGAGHLTIFIQIILPLAK
                     PILVFVGLVSFTGPWMDFILPTLVLRSEDKMTLAIGIFSWIASNSAENYTLFAAGALL
                     VAVPITLLFVFTQKHITTGLVSGAVKE"
     gene            complement(87228..88517)
                     /locus_tag="QBF46_000092"
     CDS             complement(87228..88517)
                     /locus_tag="QBF46_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDQ6672336.1"
                     /translation="MNTQQIKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAPNHIPPKNLVDWVGIKNFIMLFKFKIWSDTFLGVALWTFIWAICATIFTFSFGFIL
                     ALALAKKNIRFAKVWRLIFILPYAIPTFVTLLIFRLLLNGIGPVNSFLNSIGINSVDF
                     LSVPLNAKITVIAVCVWVGAPYFMLLIAGALTNISRDLYEASEVDGATKWQQFKEITL
                     PMVLHQVAPTLVMTFAYNFNNFGAIYLLTEGGPTNPEYRFAGHTDIFITWIYKLTLNF
                     QQYHIASVISIIIFIFLSVIAIWQFRRMKSFKDDVGM"
     gene            complement(88520..89752)
                     /locus_tag="QBF46_000093"
     CDS             complement(88520..89752)
                     /locus_tag="QBF46_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6672337.1"
                     /translation="MKLSNIVTVIILAISSTLTPQAMADKLIPETDAELLVWSDATSV
                     EYMKYAAKEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSADRIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLSAAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKSGNDPKALGIASS
                     EAKEGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRSFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PVDSLMKKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPG
                     EVLNHAHTIIEEQLSLQE"
     gene            90025..91062
                     /locus_tag="QBF46_000094"
     CDS             90025..91062
                     /locus_tag="QBF46_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDQ6672338.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHTDEFNHGKINDRGIYTETYYEPIIKYAFENAGNIYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQNIGDDWIAKIEYKHAWEKEHKNFYDPRDQEKYNVLNQ
                     DNIYVHAFYRF"
     gene            91081..>92018
                     /locus_tag="QBF46_000095"
     CDS             91081..>92018
                     /locus_tag="QBF46_000095"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDQ6672339.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYYKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGKGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQ"
BASE COUNT        22176 a        24102 c        23175 g        22565 t
ORIGIN      
        1 tgctgctgct ggccggtttc gtcacgatct ttgacctgtt cgtggtcaat gtcgccattc
       61 ccagcatgca ggccgatctg ggcgcgaact tcgcgcagat cggcttcatc gtcgctggct
      121 atgaactggc cttcggcgtg ctgctgatta ccggcgggcg gctgggcgac ctgttcggga
      181 gacggcgtct gttcgtcacc ggcatggccg gcttcacgct ggcctcggcc ttgtgcgggc
      241 tggcgcccag cgcggaattc ctgatcggtg cgcgggtgtt gcaagggctg gctgccgcct
      301 tgctgttccc acaggtttat gcgtccatcc gcgtcaactt cgacggtgac gacagccgcc
      361 gcgcctttgg cctgctggga atgacgctgg gcctggcggc catcgccggg caggtgcttg
      421 gcggctggct ggtgcatgcc gatctgttcg gcctgggctg gcgcagtatc tttctcatca
      481 acgtgcccat tgggctgttc gccatcgcgg cggcacgcac catccccgag tcctgcgcac
      541 cgcagcgccc ggcgttggac tggatgggcg tggcgttggt cagcgccggg ctgacgttgc
      601 tgctggtgcc gctgatcgag gggcctgggc agggctggcc ggcgtggagc ctgttgtcgc
      661 taggtgtggc ggtgctgctg ctggccctgt tccatcggca gcaagagcaa cggcgtatgg
      721 cgggcggctt gccactggtg gacatgcgcc tgttggccca gcgccgcttc gcgctgggcg
      781 cggtgctggt gctgctggtc tattccacgt ccagttcctt cttcctgtgc ttcgccttgc
      841 tggtgcagac cggactggga cttgaccctt tcatggcggg cagcatcttc gcgccgtgca
      901 gcgtgggctt cgtgctggca tccctggccg cgccacgttt ggtggcgcgc tgggggacgc
      961 gggccatcgt ggcgggtgcg atggtctatg cggtgtccat cgggctgctg atcgcacagg
     1021 tgtggatggc tggcgcggag ctggtgcccg cgcggctgat ccccgtgctg gtcgtggttg
     1081 gcgctggcca agggttcatc atgacgccgc tgctgaatct ggtgctgggc tttgtcgatg
     1141 aagcgcaggc cggcatggcg tcgggcgtga tctcgaccgt gcagcaggtc ggtgcggcgc
     1201 tgggtgtggc cgtggtcggc atcctgttcg cagcggcgct ggctgccgat gaaagtgttg
     1261 ccgcacaggc cggcgtgtac gcctcggcct tcgtggccgg catgctctac aacctcggtg
     1321 cagccctgct ggtctgcgtg ttgttgctga tgctggcgag aacgcagcgg ttagcgggct
     1381 gacgggaggg cgcgcgatgg agcttcggca tctgcgctgt ttcctggctg tggcagagga
     1441 actccacttc gcccgggccg ccgagcggct gcacatcgag cagtcgccgc tgtcgcgcac
     1501 catcaaggaa ctggaagaag acctgggcga acagttgttc atccgcacca gccgcagcac
     1561 acagctgaca cgggcgggca agctgtttct ggaacatgtg ccgcgcatct tcacggcctt
     1621 gcagcaagcc cgcgacagcg tgcaggcagc ggccaacggc tttcatgggc aattgcgcat
     1681 tgccctgtcc gacggcatta cgccttcgcg cctgccggcg ttgctggcgc tgtgccggca
     1741 ggaggaaccc gaagtagaga tccggctgtt cgaggtgccg ttgtctcagc agatcaaagg
     1801 gctgcatgac gacctgtacg acgtgggttt cgcgcaggcc gatgaagtgg gcgatggcgt
     1861 cgtggctcaa gcagtctgga gtgatcccct gatggtggca gtgcctgctc ggcatccgct
     1921 gctcaggcac aaacgcatcc cgctggaaga ggtgctgcgc catcctctgg tgctgtgcga
     1981 tccacaagca tgcgaaggcc atgcgcgcca ggtcgagcgt gtgctacgcc gggcggacac
     2041 ggaaccgctg attgccgagc gtgtgtcttc gtgcgatttg atgatggccc tggtatcagc
     2101 gggctttgcg ctggggatca ccggagcacc gcacattgcg gccagccgcg aatccggcgt
     2161 tgtcgcccgg ccactcgcgg ggcgtgcgcc catgctgacg acctatgtgc tgcatcggga
     2221 aggcgaatcc tcggacgtgt tggagcgctt catcgagcgc gtgttggcca tcgaatcacc
     2281 agaaggctac agatctacat cacccgttga acccgactcc ctggaggaca acgatccatg
     2341 accatgaagc tgattacccc gttcttgttg gttgctgcac ttacggcctg cggaccgtcc
     2401 gagccgccga aacagcaagc caatgtgcct accgtcgaag aactggctgc cgcccctgaa
     2461 cgcttgaagg agctgcgccg ccaatgcaag accgagcgtc cgacgatggg cgatgtgctg
     2521 tgcaaccgcg tggccgaagc aacgaacaag cgcttcttcg gagacggcaa ggtgccttac
     2581 acgccatcgg agacgccgcc gaagttctga ccgttggcgg tttgccgcat atcctgactt
     2641 tctttctcga cacgccgcag cgcgctcaag cctgcggcgt ttttactggt ttcgtccctg
     2701 caggaaacca tgctttttgt atcaccttac tgccgataac ggtctttgac cggcactgaa
     2761 ccgacaccga tcctgacgcc tgcggcacgc ctttgtgccg cgttttccac ggggaatcag
     2821 cgcaggagaa atcggaggcc agggtatgca agctcagggt gtgttgttcg ggcagatcgc
     2881 cgccgtgttc ggcattgtga tcgccggtgt gtggggtgcc acgcaatgga cagccgccgc
     2941 attgggctat cagctacgcc tgggctcgcc ctggttcgac ttgtactgca cgccggtcta
     3001 ttacccgtgg aagctgttcg agtggtggtt cttcttcgac gcctacgcgc cgcaggtctt
     3061 cgacactggc ggcatgatcg cggcgggcag cggcctactg gccgtggcgg tcgccatcgc
     3121 catgtcggtg tggcgctcgc ggcaatcgcg cctggtcacg acctatggct cggcacgttg
     3181 ggccaatgcc gccgacattc gcaaggccgg gctcacgcag cctgctggtg tctttctcgg
     3241 cctgcatgcc gggcagtacc tgcgccatga aggcccggaa cacgtcctga cgttcgcgcc
     3301 cacgcgctcg ggcaagggcg taggcctggt ggtgccgacg ctgctgtcct ggcccgcgtc
     3361 cgcagtcatc cacgacatca aaggcgagaa ctggcagatc acagcaggct ggcgctcgcg
     3421 cttctcgcat tgcctgctgt tcaaccctac cgatgcgaag tcggcagcct ataacccact
     3481 gctggaagtc cggcgcggcg cgcatgaagt gcgcgacgtg cagaacatcg cggacatcct
     3541 ggtcgatccc gaaggcgcgc tggagaagcg caatcactgg gagaagactt cgcacgcgct
     3601 gctggtcggg gccatcctgc acgtgctcta tgccggcgag gacaagacgc tgcgcggcgt
     3661 tgctaatttc ctctccgacc cggccagtcc tttcgagctg accttgcatc ggatgatgac
     3721 gaccaagcac ctgggtgatg cacctcaccc gatcgtcgca tccgctgccc gagaagtgct
     3781 gaacaagtcg gacaacgaac gctcgggcgt gttgagcacc gccatgtcct tcctcggcct
     3841 gtaccgcgat ccgacggtgg ccgaagtcac ctcgcgctgt gactggcgca tcgccgacct
     3901 gatcgcatcc gagtatccgg tgtcgctgta tctggtggtg ccaccttcgg acatcagccg
     3961 caccaagccg ctgatccggc tgatcctcaa ccagatcggg cgacggctca ccgaatcgct
     4021 ggatggctcc gacggcatcg agcgccgcca caagctactg ctgatgctcg acgagttccc
     4081 agcgctgggt cggctggact tcttcgagac ggcgctggcc ttcatggcgg gctatggcat
     4141 ccgtagcttc ctgatcgccc agtcgctcaa ccagatcgac aaagcctacg gccagaacca
     4201 ttcgattctg gacaactgcc atgtgcgtgt gaccttcgcc accaacgacg aacgcacggc
     4261 caaacgcatt tccgaaacct tgggcacggc cactgagttg cgcgcccagc gcaactacgc
     4321 aggccaccgg ctcgcaccgt ggctcggcca tctaatggtg tcgcgccaag aaacggcacg
     4381 tccgctgctg acgccaggcg aagtgatgca gctaccgacc gatgaggcgg tggtgatggt
     4441 gtccagcgtc gcgccgatca aggcgaagaa gttgcgctac ttcgccgacg ccaatttcaa
     4501 gcaacgggta ttaccgccgc ctgcggtggc agcaggccgc tatgccgatg tgccgccggt
     4561 acgacctgac gactggagcg acctggcaat acctgcggtt cctgcggcac cggccacggc
     4621 attcgccgat gacctggaga ccctgggttc gaccgatgac ggcggccccc gccgtcagcc
     4681 cgaactgtcc gaagccatcg cctatgcccc cgagatggat gccacgacca gcgatctgtc
     4741 gctgctcgat gacgacgaca tgccccagct gcttcccggc cagctcgatc ccgctctgca
     4801 acgcacggcg cggctggcat ctctcgaccc caacgacgga atcgacctat gagccaatac
     4861 cgattgaacc tgttcatcca gcatgagcac gccaagcggc tcgatgaact ggccgccaag
     4921 aaaggcgtgt ccaagtcgtc catcgtcgcg gcggcgctgg cgtcctggct gtcgccagac
     4981 gcgggcgacc agcgcgaggc cgccatcgcc aaacggctgg accgcctgtc gcggcaggcc
     5041 gagcgtctgg agcgtgatca gaacatccag atagaaacgc tggcgttgtt catccgctac
     5101 ttcctgaccg tcagcacgcc ggtgcccgaa gcccatcagg atgcagcccg cgcccagggc
     5161 aaggcgcgct tcgagcaatt cgtcgaacag ttgggccgcc acctgctgcg tggccgcagc
     5221 ttggtgcgcg acgtggtgga agagctgcac tcccaagatc gtgagttcgg catgcgcatg
     5281 gatgacgcgg cggcgatggt agccgcccat gaacgcacag cggagcgtac gccatgagtg
     5341 ccgttcctca aatcccgccc gaaccgcgtt catccgctgc ggcgtcccag gatcgccgca
     5401 tccagatgct gcgcacggcg atggggccgg tgattgccgc cgcgctggaa gacccggacg
     5461 tagtggaagt gatgctcaac cccgaccgga cattgtgggt ggatcggctg tcctctggcc
     5521 gcgcaccgct gggcgtcgaa ctgcccgagg ccgatggcga acgcatcatc cgcctggtcg
     5581 ccgcccatgt cggcgcggag gtgcatcgcg gccaaccgct cttgaccgcc gaactgccag
     5641 aaaccggcga acgcttcgag ggcatcctgc cgcccgccgc acccggcccg gcctttgcgc
     5701 tgcgcaagcg tgccgtgagc atcatcggtc tggatcgcta tgtggccgac ggcatcctga
     5761 gcgcagggca ggccgagttc ctgcgtcatg ccgtgcgtga gcggcagaac atcctgatcg
     5821 ccggaggcac cagtaccggc aagaccacac tggcgaatgc cttgctggcc gagatcgccg
     5881 ccaccggcga ccgcgtactg gtgctcgaag acaccatcga actgcaatgc gcggcccgtg
     5941 accatgtgcc gctgcgcacc cacgcaggcg tggtgtcgat gcaggaactg gtgcgcgcca
     6001 cgatgcggct gcgcccggat cgcgtgatcg tcggcgaagt gcgcggcggc gaagctctgg
     6061 atctggtgaa ggtctggggc acgggccacc ccggtggcat cgccaccatc catgccggct
     6121 ctgccttggg cgcactgctg cgcctagagc aactgatcct tgaagtcgcg gtgaatccgc
     6181 cccgcgccct gatcgccgag gcggtcaatg tggtgatcca catcgcaggc cgtggccgca
     6241 aacgccacgt cgaaaccatt tctcgcgtcg tcggtttcga cggcgcgggc taccgcctgg
     6301 cggatgcact ggaagcgccg cttcccgagt tgccgccggt tcctcttgca gccgctgccg
     6361 ctgcgccttc cctgaccact gaccaacctg gagaactgcc atgacgcacg ttgatgcttt
     6421 ccgtctttcc gtaaatccgt tttctcgcct gtccagcatg gcgcggttgc gccgcctggc
     6481 ccgacccgca gggcaagggc tgctactcgc cgtgttgatg ttgttgctgg cgggtactgc
     6541 gcaggccgcc ggttcctcga tgccgtggga aggtcccctg caatccattc tcgaatccat
     6601 ccaggggcca gtcgcccgca tcgtcgcggt gatcatcatc atcgccaccg gcctggcgtt
     6661 ggctttcggc gataccagtg gcggctttcg caagctgatc cagatcgtct tcggcctgtc
     6721 catcgccttc gcggcttcga gcttcttcct gtcgttcttc agcttctcag gtggggccgt
     6781 cgtatgagca cggccacaga tcttccgggc tttgaagtgc cgctgcatcg ctcgctgacc
     6841 gagccgatct tgctgggcgg tgcgccgcgc accgtggcaa tcgccaacgg cacgctggcc
     6901 gccgccgtcg ggctgggcct gcagctttgg attcccggtg tggtgctgtg gatcgcaggc
     6961 cattcgctgg cggtctgggg tgcgcgtgtc gatccgcagt tcatgcaggt cttcgcccgg
     7021 cacatcaagc acaagccgct gctggacgca tagggagaaa ccgacatgct gaaccttgcc
     7081 gaataccgcc agcggcccgc gctgctggcc gattggttgc cctgggccgg gctgatcgcg
     7141 ccgggcgtgg tgctgaacaa ggatggctcg ttccagcgca cggcccgctt tcgtgggccg
     7201 gatctggaca gcgccacgca aggcgagctg atcgccacgt cggctcggct caacaacgca
     7261 ctgcgccgtc tgggttcagg ctgggccttg tttatcgagg ccgagcgccg cgcagcggcg
     7321 ggctacccat gttccgagtt tcccgaaccg ctgtcttggc tggtggaaga agaacgccgc
     7381 gcggccttcg aggaatctgg ccatcactat gaaagcgcct atcacctgac cctggcctac
     7441 ctgccaccgg aggaatcgcg cgcccgtgcg gccaagctgc tctacgagca tgcgccgggc
     7501 gatggcgtgg actggcgggg ccgactcgat gccttcgtgg cggaaaccga tcgggttttc
     7561 gacctgctcg atggcgtgat gccggaaatc gcctggctgg atgacgcgca gacgctgacc
     7621 tacctgcacg ccacggtctc gacgcgacgc taccggttgg ccgtgcccga agtgcctttc
     7681 cacctcgacg cgctgctggc cgattcggcg ctggtcggtg gtctggctcc catgttgggc
     7741 gaccagcacc tgcgcgtggt gtcggtgcgg ggcttcccga cctcgacctg gccggggatt
     7801 ctcgacgacc tcaaccgcct gggctttggc taccgctgga gcacgcgctt tttgtgcctg
     7861 gacaaagccg aggcagaaaa agaactcggc cgcctccgcc gccaatggtt cgccaaacgg
     7921 aagaacgtcg tcgcgctgct gcgcgaaacc gtattccagc aggaaagccc gctggttgat
     7981 accgacgcca acaacaaggc cgccgatgcc gatgccgcct tgcaggagct gggaagcgat
     8041 caggtgtctt tcggctacct gacggccacc gtcaccgtca tggacacgga tgccgccgta
     8101 gctgatgaaa agctgcgcat ggtggagcgc gtcattcagg ggcgcggctt cgtcaccatc
     8161 cctgaaacct tgaatgcggt ggatgcctgg ctgtcgtcca ttcccggtaa cgcctacgcg
     8221 aatgtgcgcc agcccatcgt ctcgacgctg aacctggcgc acatgatgcc gctgtctgcc
     8281 gtgtgggccg gcccagagaa gaacgcgcat ctggatggcc cgccgctgat tgtcacccgc
     8341 accgatggcg cgacgccgtt tcggctggtg acgcacatcg gcgacgtggg ccacacgctg
     8401 gtcgcggggc cgaccggcat gggcaagtcg gtgctgctcg ccacgctggc gatgcagttc
     8461 cgccgttatc ccggttcacg catcttcgcc ttcgatatgg gccgctcgat gcgcgccacc
     8521 atcctgggac tcggcggcga gcattacgac ctgggcaatg acggggcgat tgctttccag
     8581 ccattggccc gcatcgacca cgagggctac cgcacctggg cgaccgaatg ggtggaaggc
     8641 cgcctgttgc atgaaggtgt cgccatcggc ccggacgaga aggccgcgat ctggtcggca
     8701 ctgggcagcc tcgccggtgc accggtggag cagcgcacgc tgacgggcct gtcggtgctg
     8761 ctgcaatcga acgcactgcg ccaggcgctc gcgccctatg tgctgggcgg tgcccacggc
     8821 aagctgctgg acgccgatgc cgaccggctg ggttccggca gcgtgcaggg cttcgagatg
     8881 gaagagctga tgcacagcaa ggcggcggtg ctggctgtgc tgggctacct gttcgcccgc
     8941 ttcgatgaac gcttcgacgg cgcgcccacg ctgctgatcc ttgatgaagc ctggttgttc
     9001 cttgatgacc cggtgttcgc cgcccgcatt cgccagtggc tcaagacgct gcgcaagaag
     9061 aatgtcagcg tcatctttgc cacccagtcg ctggccgaca tcaaggattc gagcattgcc
     9121 ccggcagtga tcgaaagctg cgccagtcgg atctttctcc caaatccgca ggccaccgag
     9181 ccgcagatcc gcacgatcta cgaaggcttc gggctgaact cgcgccagat cgagatcgtg
     9241 gcgaccgcgc agcccaagcg cgactactac taccaatcac gcctgggaaa tcgcctgttc
     9301 gacctcgacc tggggccagc cacgctggcc ttcgcgggtg catccacccc gcaagaccaa
     9361 cgtgacatgg atcgcgtgct gctggaggca ggcacaccgg gcttcgccgg tgcctggttg
     9421 cgccatcgcg gcctcgattg ggccgccgac ctgctgccgt cctcaccggc agcagcgtcc
     9481 ttcctcgctt ctcaacgacc ggaggtttca ccatgaagac ccaaccccgt ttgctgtccg
     9541 tctcgctcgc cgccgtgctg tcggtatcgc tgctggcctc acagcctgca tccgcgctga
     9601 cggtgttcga cccgtccaac ttcgtgcaga acacgctgac cgccgtgcgc acgctggagc
     9661 agatcaacaa ccagatcaac cagcttcaga acgaagcgca gatgctgatg aaccaggcgc
     9721 gcaatctggc gaatctggac ttcaacatcg tcaatcgcct gcgctcgacg ttggcgacca
     9781 ccgagcggct gatcgccgag gctcgcggcc tggcctacga cgtgcagagc atggatgcca
     9841 ccttcgcccg gctgtacccg gagcagtacg ccgcgaccgt cagcggcgac cagatgctgc
     9901 gcgacgcgca ggagcgctgg aagaacacgc tgaacggcct gcacaccgcg atgcggatgc
     9961 aggcgcaggt gtcgcagaac ttggcgcaag acgaaagcgc attggccgac ctcgtgagcc
    10021 agagccagtc cgccaccggc gcgctgcaag ccatgcaggc gaccaaccag cttctggcct
    10081 tgcaggccaa gcagtccatc caggcgcagc agctccagat cacgcaggaa cgcgccgcag
    10141 cgctggaact ggcacgccag gcggcggctg tcgaacgtgg ccgtgaagtg acgcgccgct
    10201 tcttgggtga tggcacgccc tacacgccgc tgcgcgtgga tttctacggc aactgaaggg
    10261 agacggccat gcgatgcgct ttcttgctgc tgcccgtgct gctggttgct tgcagccagc
    10321 agccgaccga cgacctggcc gctgccttgg ccgccgatcc cgtgcgactc aaggctttgc
    10381 gtgcgcagtg tgcagccgac aggacgacgg caggcgaata cgcttgcctg gtagcggctg
    10441 aagccttccg gcggcgcttc ttctccggtg aaaccgggcc tgacgagttc cgcacactgg
    10501 ccgatctgcc gccgatcccg ccgagcttcg acacgcccgc cagtgaccag gacgccatgc
    10561 ccttcgagag ggacgtgcca tgaacgacgt gacagtgatc gaccgatttc tcgctacctt
    10621 ctcgcgctac atcgactcgg gctttgggct gctgcagggc gaagtcgcgt tccttaccgc
    10681 cacgctgatc gtcatcgaca tgaccatcgc cgggctgtac tgggcgatga gccacgccac
    10741 cggccagggc gatgatgtga tcgccaaact gctgcgcaag gtgctttacg tcggcgcctt
    10801 cgcctacatc atcaacaact tcaactggct ggccagcatc gtcttccgct cgttcgcagg
    10861 cctgggcctg acggccagcg gctcgacgat gagcatgggc gaattcctgc aaccgggacg
    10921 cctggccaag accggcatcg acgcagccgc gccgatcctt gagcagatca gtgacatggc
    10981 gggcttcccc gaggtgttcg tgaacatgac gcccatcgtg gtcatgttcc tggcctgggc
    11041 ggtggtgatc ctgtgcttct tcgtgctggc gatccagctt ttcatcacgc tgatcgagtt
    11101 caagctgacc acgctcgcgg gcttcgtgtt ggtgccgttc gcgctgtgga acaagaccag
    11161 ctttcttgcc gaaaaggtgc tgggcaacgt ggtgtcctcg ggcatcaagg tgttggtgct
    11221 ggccgtcatc gtcggcattg gctcgggcct gttcgccgag ttccaggtgc atccagccga
    11281 gccgtccatc gaccatgcgg tggtcatcat gctggcctcg ctcacgctgc tggcgttggg
    11341 aatcttcggg ccgggcattg ccacgggcct ggtatccggc gcaccgcagc ttggcgcagg
    11401 cgcgatggcc ggtgctgcga ttggtgccgc aggcgcggcg gttgccgtag gtgccgccgc
    11461 caccggcgtg ggcggtgcag tgatggccgg ggcgcgcatg gcaccggctg ccgccaagct
    11521 cgccggggct ggcgcgcggg ccgctacttc ggcggcgggc agcgcgaagt cggctttcca
    11581 ggccggttct gccgctgcgg gcggcggagc caaaggcgcg atggcagggc tgggcagtgt
    11641 cgccaagggc ggcgcacagg cggcaggcca acgcgccggt gcaggcatca aagcggcggc
    11701 agccaaggct gccgcgccat tcaaagcagg ctggcaaggc tccggcaccg atggtggttt
    11761 cggcggtggc gctgccagtt ccgggcaaac cgccgctggc gatgccaacg cgcagaagca
    11821 ggaacaaccc ggctgggcca agcgcctgca acgccgccaa caactcaccc aggccaccac
    11881 cacggccgcg cacacgctgc gcggtggcga cggcggcggt tcgggccaag gcccgagcct
    11941 gcgcgattcc gatagctgac tttcaaggag aactccatgc gattcaaacg accgcaggtg
    12001 cgctatgccg atacgccgca gcctgccact ccgtatcaag ctgctgcgca ggtctgggac
    12061 gagcgtatcg gctcagcccg tgtgcaggcg aagaactggc gactgatggc cttcggctgc
    12121 ctggtgctcg cgctgctgat ggccggtggc ctggtgtggc gctcggccca gtccatcgtc
    12181 acgccctatg tcatcgaggt ggatcagtcc ggtcaggtgc gcaccgtggg cgaagcggcc
    12241 acgccgtacc ggcccaccga tgcgcagacg gcgcaccaca tcgcgcgctt cgtgacgctg
    12301 gtgcgctcgc tgtccatcga cccaatcgtt gtgcgccaga actggctcga tgcctacgac
    12361 tacaccactg accggggcgc ggcggtactc aacgactatg cgcgcaccaa tgacccgttc
    12421 gcccgcatcg gcaaggaatc ggtgacggtg cagatcacca gtgtggttcg tgccagcgat
    12481 gcgtcgttca acgtgcgctg gacggaacgc cgttacgtca atggcgcggc tgccgggttg
    12541 gagcgatgga cggcggtggt gtccatcgtg cagcagaccc cgcgcaccga agaacgcctg
    12601 cgccgcaacc cactgggcat ctacgtcaat ggcctgtcgt ggagccgtga actggattct
    12661 tctgaaggag taaagccatg agtgtgcctt tccgttttta cgctttgccg ttgatccttc
    12721 tgggccttgc gggctgcgcc acgcagggca aaccgccgcc ggtgatctcg cttgatgagc
    12781 cggtgcaggc gcagccattg cccgagccgc ccacgcccat cgaggtcgtg gcggtgccgg
    12841 agccgttagc actgcccgca cagttgaagc cgctgccgga cgttgatgcg gccccgacca
    12901 cgccggagtc cgccgacgag aaggtgcgcg tctcccgcgc taatgccgaa gcacggatcg
    12961 cgccgacccg tgagggctat gtcaatgcga ttcaagtctg gccgttcacc gatggtgcgc
    13021 tttaccaggt ctatgcgtcc gtaggccgcg tgactgtgat cgcgctccag cccggcgagg
    13081 aactggtgac ggtggctgcg ggcgataccg tgcgctggat cgtgggtgac acctccagcg
    13141 gcagcggtgc cgagctgcgc gtcaatgtgc tggtcaaacc tatccgttcc agcttgaaga
    13201 ccaatctcgt catcaccacc agccgcagga cgtacctgct ggaactgact tcgaccgaca
    13261 aggcatggat ggcgtcggtg tcctgggact accccaagga tcggatgctg gccttgcagc
    13321 gccaggcaca ggctgccaac gctgccgcgc caatcgatgc gggcctgtcg ctggagaaca
    13381 tccgctttcg ctacgcgatc agcggcagca atccgccgtg gaagccgctg cgggccttcg
    13441 atgatggcga gaaggtctat atccagtttc cggcgggcat cgcccagggt gagttgccgc
    13501 cgctgttcgt gatcggtgct cagggcgatg ggcaactggt caattaccgc ttccgctcgc
    13561 cgtactacat cgtggatcgg ctgttcggtg cggccgaact gcgcctgggg gctgacaagg
    13621 gcgacgtggt gcgcatcgag cgcaccgatg gcgtcgtatc aaggagtggg aggaactgat
    13681 catgagccag gaagatactc ccgaccttgc cgcgccgcag gtggacaagg tggcacccga
    13741 ggcggtggcg ctgcgcgccc aaccacgccc ggtcacgcgc ctgaaccggc gcacgctggc
    13801 gatcctcgcc ggcggcttgt cggttggcgt gatgggcgca ctgatctggt cgctgcaacc
    13861 gcagcaacgc ggcgcgggcg aatccaccga gctgtacaac gtggatcgcg tgtcgaagtc
    13921 tgaagggctg gatgcgctgc cgacagacta ttccaagctg ccagcgctgc cgcttgacgt
    13981 gccggaactg gggccaccgc tgccgggcga tcttggcccg gccatcgtga actcgcagca
    14041 gcctgtcgcc gccacctatg ccgcgcccgg ctacgacccc aacgatgcgc tgcgcaagga
    14101 agaagaagcg gcggcagctt catcggtgtt cttccgtacc ggctccccgc aggccgcgcc
    14161 cgtggcgcag tcgcaggtcg ctgccgcgcc gggctttgcc gccaatgccg ctttcgatcc
    14221 gctggccgct ggcccggcct cgacggcggc ccagcctgct gatccgactg ccgtgcagaa
    14281 ccggcaagac cagaaagagg cgttcctggc aggcggctct acggaaaccc gtaactccgg
    14341 caacctgcaa atgcccacct cgccgtatca ggtgatggct ggaaccgtca ttgccggggc
    14401 actggtcacg ggcatcaaat ccgacctgcc tggcgacgtg atcgccacgg tgacggaacc
    14461 gatctacgac acggccaccg gcaagttcct gctgatccct cagggttcgc gcatcttggg
    14521 caaatacaac agccaggtca gttacggcca gagccgcgtg caagtggtgt ggaaccgcat
    14581 caccctgccg gacacgtctt cgctcacgct cgacaacctg gtcggcaccg acccggcggg
    14641 ctatgccggc ctggaagacg acgtggactg gcactgggat cggatctttg caggggccgt
    14701 gctgaccacg ctgctgggtg tgggagccga actggccgcg ccggagaacc gtcaggatgg
    14761 cgatcgcgtc atcatcgctg ggcgcgacag cctgcaggac accgtgaacc aggtcggcca
    14821 ggaggtgacc cggcgcaacc tcaacatcca gcccacactg accagccgcc cgggcttgcc
    14881 ggtgcgcatc atcgtcaacc gcgatctggt gctgcggccc tatcagccgc tctttttcaa
    14941 caaagggact tcgcgatgaa cacgaccaaa aaactgcggc ttggccctct gcctcgccat
    15001 gaagtcacga agctgacctt ctcttgcccg gccagcctga aagccgacct cgatcgttac
    15061 gctgcgctgc acgcgcaggc gtatggcgag gcggtggatg ccactacgct gatcccgcac
    15121 atgctggagg cgttcatggc gggggatcgt gggtttagaa agggagatgg gtagaagtaa
    15181 caaaaatatg cgcaaaattc tataatgtcc gctattggcc aggtgcaaaa gttcaaaaaa
    15241 tgcaattgaa ggtctgtttt gcagcgactg ctgacgatca gctttctctt gttttcctat
    15301 catgccaaaa gggattaatt tggaggcgag gatgctggag gtagacaaga aacgcgaagc
    15361 gacagcttcg atcagagggt atttttacca acttgacgct gccttgctcg aaatcctaaa
    15421 cgcggacctg agcgatgaaa tcgttatcga gggaattgaa gacttcgacc gctacaccga
    15481 tgaaagcgtc atttacggcc aggtgaagta ctacgcggag cagaacctga cagactcggt
    15541 actgcgtgcc cccctgcata agctctttgt tcatttccac gggctcgaag aagcacagcg
    15601 ggcaggccga aaatacctgc tgtacgggca cttttcagaa gtaaagatcg atattggtga
    15661 gctttccgtc gagcgcttca agtcggtgat ggaataccga aaggaagtga aggccgctga
    15721 cggtacaaaa tcctacgaaa agaagtcttt gctcgacggc atggccgcgc cagataggct
    15781 aattgaggcg ttctgcaaga gcttcagcat acaaatctcg aaggaatttt ctgaacatcg
    15841 caggctcgtc attgaggcaa tacggaaaaa ccaaagcgtc tcaggggttg aggccgaagg
    15901 ttttcactat ccaatggcct tcgactacat cgcaacactt gccaccaaaa aagatcacaa
    15961 cgaccgtaag gtcacccgtc gtgatctgca ggaactgctc aaaggtgctc aagctatcca
    16021 taatcgttgg cttctccggg agaaggatgc aagcgagtac gcgaagcata ttaaacgcct
    16081 gtacttctcg ctcacgaacg gcgcaggaat cgcccgtgtt ttcatcatcg aatgtgatgg
    16141 ggcgactgac ggctcggtca tctgcgacca actcagagca attggtaata attggagttc
    16201 tgctaaaaag cgccgcatcc agaattccga gcgctacgcg ccattcatcc tgctacgcgg
    16261 cgcggatgaa acactcatct tgcaagtaaa aaatgagctt ttcgatactg gaaccgtttt
    16321 cgtagacggt tacccatatc gcggatcgcc ctttcgcaac gatcacgttc agagcccgca
    16381 gaccgaagta caccagatcg aaattcgttt tgtcgaagac gttgaccaac tgctggaagc
    16441 tctcaacgga gtgggacgga agttgtgcca catctatgat ttcttcctgg aacgcccagc
    16501 agccatggct atgccgggtc caaagagtcg gatgtattcg atccccgtta gcagcatctc
    16561 cacaatcaat aaaattatct gaggacgaac tatgactgag gaagtcgcca atgccgaggt
    16621 tgtcgccgtt taccccaaca aggttcgtgt ggccgtcgat gatcttttga actttcaagc
    16681 agcgggggag tctcttcgtg ttgggtcttt tctcaaagtc tcggacaatg acaacgtttc
    16741 tcttatctgc atcatcgaaa gcttttcaat cgagatgaaa gaggtgaaag gcgacggtat
    16801 cagtgaccac aaacgcgttt atatgattga ttcctatccc ctcgggaccc ttaaagatgg
    16861 aaagttcaat cgcggtggtg acgaactcgc cattccgcca aagaaggtcg tcccggcaac
    16921 gaaagcggaa atcgcagcta tctatgcaga ctgctatgcc gctgaagacc gcttttgctt
    16981 ttcgtcactg actaggcaac gtgatatcgt cgtacctgtt catggcgaca ggttcttcaa
    17041 taaacatatt gccgtcgtcg gtgcttcggg atcgggaaaa tcgtcgagtg tgacgtcagt
    17101 tctgcagaaa gcggtatctg ctaaaagcgg cgaataccaa ggtctcaaca actctcatat
    17161 cctaatattc gatcttcatt ccgaatatgc tacggctttt ccaaaggcga atatcatcac
    17221 tgtcgatgac ctcgtcctgc catattggct tctcaacgaa gacgagatgg aggacatgtt
    17281 cctagagtcc ggcgacaata acaactacaa tcaggagtcg ctgctacgat ctgtggtgac
    17341 cattgccaag aagatcagca atcctgctgt cgaaaaggtc aacttcgaca gtccgcttcg
    17401 atatgacatt cagcacgtag cgaacgcttt ccgcaacctg acgcgggaaa caaaagacgc
    17461 tgaaaactcg ctgcatatca agtttaagga tggtacgacc gaaacgttcg atgatgttga
    17521 gggtcagctg gagcactatt gcaaggcttt gcgcgagttc gctgctccgg aacggagtaa
    17581 aatcaacaag ggcccctatg ccgacggcag catcgacaaa tttgagcggc ggattagatc
    17641 aaaaatctcg aactctcggc ttgacttcat attcggtgcg aaggccaaag ctgcgaccct
    17701 agagcaggta cttgaacagt ttaccggcta caaggcggga ggcgagtcga atgtcacaat
    17761 catcgacctc agcggcattc cctttgaact gctcagcatt accgtttcgc tgatctcccg
    17821 ccttatgttc gatttttgct actacgctcg tcgcgtggag agtaaaacag agcagccgtt
    17881 cctgatggtg tatgaggaag cgcacaaata cgcgccaaag agcggtcttg cccgctacaa
    17941 ggcctccttg gctgcaatcg aacgcattgc caaagaagga cgaaagtacg ggatcagcct
    18001 tctgatttcg agtcagcgac catctgaaat atcagagact atcttttcgc aatgctccaa
    18061 cttcttggca atgcgtctta ccaaccccga tgatcaaagc tatgtccgcc gactactgcc
    18121 ggatacgctt ggcaatttga ccgaaggcat gcccgcgctg gaacaagggg aggcgcttct
    18181 gatcggggac gccgtcatca tgccgtgcgt cgtgtatgtg gaaaaatgcc accccgaacc
    18241 ctcttctagt gacgtgaaat atctcacgct ctggaaagcg gagtggaagg agatcgactt
    18301 caaaaagcgt gcagataact ggcgaggatg atggagacat cgttgcacaa attaagtttg
    18361 aggcgaacta cccaaaaccc tagttgtggc aataccacgg tgcaaaacga acttgcaaag
    18421 aaggttcaat gcaatgactg ctttgagtgg cagcagtcat tggccttgtt gagttgaagg
    18481 gcagcaacca tccttaaccg gacattgtga atcagcctgt gaagttccgc tttggggcga
    18541 aagcggcagt cttctattgc actttctgtt gtgccttgtt tttttaggtg cgtgaaagaa
    18601 tcagatgcta tgacgaatag gctcgcaatc tctgagtttt ttcaccgccg cagcgcgatg
    18661 tggcttgctt tctcaaagct ctcatgtggc tcccagccca cagccccgca tcacccgcaa
    18721 gcggcgcgaa acacggctaa cctattggcc tgtatatgat ttcaagcccc gtgcgatttg
    18781 cacgaagggc ttgacgccgg ccatttttta tggctgccac agtggcgaag tcaacaacat
    18841 gcaaatttga gtacctcgcg cgccttacat actcggggta tctggcgttc gccgcttgcc
    18901 tggcaggtca cattgtttct tgggatcaac tcactggtct attacgtgat tattggctgg
    18961 cttccggcga tcctcatcag tcacggctat agcgaagcac aggcgggttc actgcatggt
    19021 ttgctgcaac tagccacagc agctcccggt ttgctgatcc ctcttttctt acatcatgtg
    19081 aaagatcagc gtggtattgc agcgttcgtt gccttgatgt gcgcagtggg cgctgttggg
    19141 ctctgcttta tgccagcgca cgcgatcacc tggactctgc ttttcggttt tggttccggc
    19201 gcaacaatga tactggggtt gacgttcatt ggtcttcggg caagttctgc gcatcaggcg
    19261 gcggcactct cggggatggc acaatccgtc gggtatttgc tggcagcctg tgggccgccg
    19321 ctaatgggta aaatacacga tgctaacggt aactggtccg taccactgat gggtgttgca
    19381 atactttcac tactgatggc gattttcgga ctttgcgccg ggagagacaa agaaattcgc
    19441 taatatccgg tgctatagtg acgtgacaaa tcatgcgtga aagggagaac aaacacgatg
    19501 aatattcagt gcaaacgcgt ttatgatccg gctgaacaga gcgatggtta tcgcgtactg
    19561 gtcgaccgcc tctggccgcg cggtatcaaa aaaaccgatt tagcccttga tgagtgggat
    19621 aaagaaatca cgccgtcaac ggaactgcgc aaagcctttc acggcgaagt cgtcgatttt
    19681 gcaacctttc gcgagcaata tcttgcagaa ctggcgcaac acgagcaaga aggaaagcgg
    19741 ctggcggaca tcgccaaaaa acagccgctg accctgctct acgcagcaaa aaacaccacg
    19801 cagaaccatg cgctggtgct ggccgactgg ctacgtagct tgtgatttta gtacagcatc
    19861 cggcggttat ttttcaccag ccggatggtc ccgtcgccac aatgcccatt catcaatcgt
    19921 ttcaccgccc ggtaatttgc aattgttgct gaccccttgc gctgtcgaca ctggaatgag
    19981 cgtcccgccc ttctgctggc aatagaccga cgccggattt gccataccaa tctgcggcgg
    20041 tttaggtgct tctggctgag aaggggttga acaaccagcc aggaccagca agcaaggcag
    20101 aacaaaactg ataattttca tttattgatc tcacatattt atccaagctt agagtatcgc
    20161 ggtatcgttt tgttttgcag cactattttt attacattca ctcaaaacat attacgtctt
    20221 gtttcatctt tgttgatgat gttttatcat gcctgcaaag attaaataat cagcacttac
    20281 ccgccgtatc ctgcagttgt tccgtgtcag atcagattat cgcccgcgtc tcgcaatccc
    20341 ttgccaaaga acagtcactg gaaagtctgg tccgacagct tctggagatg ctggaaatgg
    20401 tcactgatat ggaatcaacc tacctgacca aagtggatgt cgaagcgcgc ctgcagcata
    20461 ttatgtttgc ccgtaacagc cagaaaatgc acatcccgga gaattttacc gtctcgtggg
    20521 attactcgtt atgcaaacgc gccattgatg aaaactgctt tttcagcgat gaagtccctg
    20581 accgttgggg cgactgtatt gcggcacgca atcttggcat caccacattt ctgagcacgc
    20641 caattcactt accggatgga tcattctatg gcacgctttg cgccgccagc agtgagaagc
    20701 gccagtggag tgaacgcgcg gaacaggttt tacagttatt cgccggactg attgcacaat
    20761 atattcaaaa agaggcactg gttgaacagc tgcgcgaagc caatgctgcg ctgattgcgc
    20821 aatcgtatac cgactcgtta accgggctac cgaatcggcg ggcgattttt gaaaatctga
    20881 cgacgctgtt ttccctcgcc cggcatctta accataagat aatgatcgcg tttatcgatc
    20941 tggataactt caaattaatc aatgatcgtt ttggtcataa tagtggcgat ctgtttctca
    21001 ttcaggttgg cgagcgcctt aatacgctcc agcaaaatgg cgaagttatt ggtcgtctcg
    21061 gtggtgatga gtttttggtt gtttcactaa acaacgagaa tgcggatatt tcgtcgctgc
    21121 gagaacgtat tcaacagcaa atacgtggag aatatcactt aggtgatgtt gatttgtatt
    21181 atcccggtgc cagtcttggc atagtagaag tcgatcctga aacaaccgat gcagacagtg
    21241 ccctgcatgc tgccgatatt gcgatgtacc aggagaaaaa acacaaacag aaaacacctt
    21301 ttgtcgcgca tccagcgcta cattcctgag gcgtattcac atccttttga ttggtgataa
    21361 catgcgaatc ggtattattt ttccggttgt aatcttcatt acagcggtcg tatttttagc
    21421 atggtttttt attggcggct atgctgcccc gggagcataa agatgaaaaa aacaacgatt
    21481 attatgatgg gtgtggcgat tattgtcgta ctcggcactg agctgggatg gtggtaacgt
    21541 cacctctaaa aaatagcaaa ggctgcctgt gtgcagcctt tgtgcaattt acgcgttaac
    21601 tttaatcttc ctgtagataa atagcacgac aatcgcacca ataacggcaa ccacgaagct
    21661 gccaaaattg aagccatcga ctttaccaaa gccaaacagc gtgctgatcc atccgccgac
    21721 tacagcaccg actatcccca gcaggatagt cataaagaat ccaccaccat ctttacctgg
    21781 catgatccac ttcgccagaa taccggcaat aagcccaaaa ataatccatg acagaatgcc
    21841 cattgtttcc tcacttatct gttttgcatt agcgggttag tcgctgataa aaagcatagc
    21901 acaacatcgg gagggcaaga tttgtgacga gcatcacgga ggattttttt gcgatggcgc
    21961 agaaattgcg ccatcaacga tcagtgataa ttaccaacca caaacatcat gttcgttttc
    22021 cgtgtcataa gaacgcacgg tattcaccag atcttttatc acttcagccg ccacttctgg
    22081 caccagcaaa gtcatcggcg tctctgtttc ataatcgaca gaaacgccat tactgttatt
    22141 ggtgacggtc acggtatacg ttgctttgcc catgattcat ttcccgttat gaatgacttt
    22201 ccgttgttgc gcaccttcca tcaggacttc aggagccacg aagaagtcaa tgttgaaata
    22261 agtatcgtca gtcatggctt caatgttgtg ccacttttct ggagggaaca ccgcaaactg
    22321 ccccgcttcg ataaggatca cctgatcagg ctctgcacta tgttcatcag cgtagccgag
    22381 atatttgacc gccccatgca taacggaaag gcgtgggtaa acccccgggc gcgttccttt
    22441 atcaagatga cgttcgaata tgccggcagg cgcagtttgt ttattccaga aaggcgttga
    22501 gcgcgtatga atataattct gtgggatttg cagcatcctt ttccctcctt cggtgaatgc
    22561 gctgaaaacg gtttattcca gccgtttcag ggtacgcctg ataatttgca ttttaaatac
    22621 catttattgg ttacttttta gcaccatatc agagaagaat cagggaggat tatagatggg
    22681 aagcccgcga caacgcgggc tgaaagcatc aggattgcag cgtcgccagt cgggcagcga
    22741 aacccacgaa catcaaacca atcagtgagt tgccaacttt agccagtttc tttttggtac
    22801 gtatgtactg cgtgacaaaa gcaccagata taatcaggaa gctcaaatag cagaaactca
    22861 ccagttccag cgtcgtcgcc agaataaaga atgaaattcc cgtatgtggg gcattaacat
    22921 cgataaactg tacgaaaaac gacacataga acaaaatggc tttcggatta gtcaggctca
    22981 aaattaacgc gcgtttaaaa atagcaccgt attggggctc atcggatttg gcctcgctat
    23041 ttttaccctt cagggtcgcg taaagaattt tactccccag atagagcaaa taaaacgcac
    23101 caagataacg tacaatgttg aataatatcg gggtggtctt aattaatgtc gccactccag
    23161 cccatgccag aaacatcaat accgcatcgc caataaatac accgcaggcc gcaagataac
    23221 cgcctttcat accgctactg acgctatttt tgagtacaaa cagggtattt ggccctggca
    23281 ccaacacaat aaaaatggcc ccaaccagat aggtccagta attcagaacc ccgtattcag
    23341 cgaacacatt aacctcttta attatctttc gatcatgcgc gattaaaggt gaatatgcta
    23401 accaatctgt agcggcttag aaaggagaaa atcaggtttt aacctgatat caacccgata
    23461 attgaatcat taatcaggca tgctccagtg aaaaattcgg gtagtgctcg gcaaaatact
    23521 ggcgtaaaaa ttctaccgtt atccgcactt tcgctgacgt cgccagcctt gaaacataaa
    23581 cggcccagac gttcgctggc tggtaatatt ccggtagcac ttgcactaaa tgaccactgg
    23641 caatgttttc gctaacatcc caccaggaac gcagagcaat cccctgccca tcgagacacc
    23701 actgatggac aatttctcca tgattagacg acagtgggcc ggtgacttta atcgcatggg
    23761 ggccttcttt attgcgtaat tgccagacac caaaaggatg atcgcgctct ttaatgacca
    23821 ggcacggcaa tgcagaaaga tcggttaaat gtttcggcgc gccatgttgc gcaataaatt
    23881 ccggcgacgc gcagagaata cggtaattgg tcgccagttt gcgggcgatt aaattagggg
    23941 cgatgtcatc gccaatgcga atatcgagat cgacaccttc attgaccaaa tcgaccagtc
    24001 ggtcttccac atcaaaacgt aattccagtt gcggatacgc cttcgccagc gctgataacg
    24061 ccggagccac cacctgtcgt ccaaaaccaa agctgctgat aatacgcagc atcccctgcg
    24121 gcacctgacg cacgtcagaa agttcgtcca tcatctgacc gacatcctgc aaaatccgct
    24181 gcgcccattc ataaatccgc tctccttctt cggtaatggt gacgcggcgg gtggtgcggt
    24241 gtaacaacac cacgtttagc gtttgctcca gcaaggcgat gcgcttgctg acgaacgccg
    24301 gtgaaacgcc cagttcttcc gccacggcgg caaaaccagc ccggcgagcc accagcataa
    24361 agacgcgcaa atcattcagc agcggtaaat tattcatgat tcgtgtttta tgtttcacca
    24421 gttacgggga ttaattcctt ttcagtcaat tataggatgg tgatgttgtc aattttgatg
    24481 gtcaggaagt gagaacccaa tgatgaaaac gatgcgtatt gctgcgatcc cgggagacgg
    24541 gattggcaaa gaagtccttc ctgaagggat tcgcgtgtta caggctgccg ctgagcgctg
    24601 gggcttcgcc ttgagttttg agcaaatgga gtgggcgagc tgcgagtatt acagccatca
    24661 cggtaaaatg atgccggacg actggcatga gcaacttagc cgtttcgacg ccatctattt
    24721 tggtgccgtc ggctggccgg ataccgttcc ggaccatatt tcgttgtggg gttcgctgct
    24781 gaaatttcgt cgtgaattcg accagtacgt caacctgcgc ccggttcgtc tctttcctgg
    24841 cgttccctgc ccgctggcgg gaaaacagcc tggcgacatc gatttttacg tggtcaggga
    24901 aaacaccgaa ggcgaatatt cctcgctcgg cggtagagtg aatgaaggta cagagcatga
    24961 agtcgtcatt caggaatcgg tatttacgcg tcgtggtgtc gatcgcattt tgcgttatgc
    25021 cttcgaactt gcgcaaagcc gcccacgtaa gacgctaact tctgccacta aatcgaacgg
    25081 tttagccatc agcatgccgt actgggatga gcgagtggaa gcaatggccg agaattaccc
    25141 ggagatccgc tgggacaagc agcatattga tattctctgc gcgcgttttg tgatgcagac
    25201 ggaacgcttc gatgtggtgg tggcgtccaa tttgtttggc gatatccttt ccgatcttgg
    25261 cccggcctgc accggcacca ttggcattgc cccatccgcc aacctgaatc cggaacgcac
    25321 tttcccgtcg ctcttcgagc ctgtccacgg ttccgcgccg gatatctacg ggaaaaatat
    25381 tgctaaccct atcgccacga tttgggccgg ggcaatgatg ctcgattttc tcggcaatgg
    25441 cgatgagcgt ttccagcaag cgcataacgg tattctggca gcaattgaag aagtgattgc
    25501 tcacgggccg aaaacacctg atatgaaagg caatgccacc acgccacagg ttgccgacgc
    25561 gatttgcaaa attattttgc gttaaggtca aaccagttta tttgaaccgc gtcactgacg
    25621 cggttttttt attcgttctt tgcagtaaat aacctgcgtc atttcacctt ttattgtttc
    25681 cgtttcgtgt tttatggctt tccgtattct taattgttta atttatgtaa catgcaaata
    25741 atcaatttgt taaacacaac atacaactca ttaccctgca aataattaca actggcaaaa
    25801 gccagagacg ggcttttatg cccaggagat gactcatgat gagcaatgtt aagaaaaaag
    25861 atgtgccgct gataagcatc agcctggtgg ccattctttt catcgcagct gcattaagcc
    25921 ttttcccaca acaatcggcc gacgcggcca acgcaatata cacttttgtt acgcgtacgt
    25981 taggttccgc cgtacaggta ttggttttgc tggcaatggg actggtgatt tatttagcca
    26041 ccagtaaata cggcaatatt cgtcttggcg aaggaaaacc ggaatacagc acgctctcct
    26101 ggctgtttat gtttatttgt gccggtttag gttcttctac gctttattgg ggggttgctg
    26161 aatgggccta ttattatcaa acacctggat taaatatcgc accgcgttca caacaggcac
    26221 tcgaatttag cgttccctac tctttcttcc actggggcat cagcgcctgg gcaacttata
    26281 cgctggcctc attaatcatg gcttatcact ttcatgtgcg gaaaaacaaa ggtctgagcc
    26341 tttccggcat tattgctgct attaccggcg ttcgcccgca aggcccatgg ggaaaactgg
    26401 tcgatttgat gttcctgatc gccactgtcg gcgcactgac catttccctt gttgttaccg
    26461 cagcaacctt tacccgtggg ctttccgcgc tgaccggttt acccgataac ttcaccgtgc
    26521 aggcatttgt gatcctgctt tccggcggca ttttttgcct aagctcgtgg attggtatca
    26581 acaacggttt gcaacgtctg agcaaaatgg ttggctgggg cgcgttcctg ctgccattac
    26641 tggtgctgat tgtcggccca accgaattta ttaccaacag catcatcaat gccatcggcc
    26701 tgaccacgca aaacttcctg caaatgagct tattcaccga tccgcttggc gatggttcat
    26761 ttacccgcaa ctggaccgtt ttctactggc tgtggtggat ctcatacacc cctggcgtag
    26821 caatgtttgt cacccgcgtt tcccgcggtc gtaagattaa agaagttatc tggggactga
    26881 tcctcggcag caccgtcggt tgctggttct tctttggcgt aatggaaagc tatgccattc
    26941 atcagtttat caatggcgta atcaacgtcc cacaggtgct ggaaacactg ggcggcgaaa
    27001 cagctgtaca gcaagttctg atgtcgttgc cagccggtaa attgttcctc gccgcatacc
    27061 tgggcgtgat gattattttc cttgcctcgc atatggatgc agtggcctac accatggcgg
    27121 cgaccagtac gcgtaatctc caggaaggtg acgatcctga ccgtgggctg cgtcttttct
    27181 ggtgcgtggt gatcactctg atcccgcttt ccatcttgtt taccggtgct tcgctggaaa
    27241 cgatgaaaac caccgtcgtg ctcacagccc ttcccttcct cgtcatttta ctggtgaaag
    27301 tcggcggatt tattcgctgg ctgaaacagg attacgccga cattccggct catcaagttg
    27361 aacattatct cccgcagaca ccggttgaag ccctggaaaa aacgccagtg ctccctgcgg
    27421 gaaccgtatt caaaggcgac aactgagcgc catcatccta acgataaagg tatccctatg
    27481 agcaatctga gccctgactt tgtactaccc gaaaattttt gcgctaaccc gcaagaggcg
    27541 tggaccattc ctgcccgttt ttataccgat cagaacgcgt ttgaacacga aaaagagaac
    27601 gtcttcgcca aaagctggat ttgcgtcgct cacagcagcg aactggcgaa tgccaatgat
    27661 tatgtgacgc gtgagatcat tggcgaaagc atcgtgctgg tacgcggtcg tgataaggtt
    27721 ttgcgcgcgt tctataacgt gtgtccgcac cgtggtcatc agttgttgag cggtgaagga
    27781 aaagcaaaaa atgtgattac ctgcccgtat cacgcatggg cattcaaact cgatggcaac
    27841 ctggcccatg cacgtaactg cgaaaacgtc gccaatttcg atagcgacaa agcgcaactg
    27901 gttccggtgc gtctggaaga atatgccgga ttcgtcttca tcaacatgga ccccaacgcc
    27961 accagcgtag aagatcaatt acccggcctg ggcgcgaaag tgctggaagc ctgcccggaa
    28021 gtccacgatc tgaaactggc ggcccgcttt accacccgca cgcctgccaa ctggaagaac
    28081 attgtcgata actatctcga gtgctatcac tgtggtccgg cgcatccagg tttctccgac
    28141 tccgtacagg ttgatcgtta ctggcacacc atgcacggta actggacgct gcaatacggt
    28201 ttcgccaaac cgtccgaaca gtcgtttaaa tttgaagagg gtacggatgc ggcattccac
    28261 ggtttctggc tgtggccgtg cacgatgctg aacgtcaccc cgatcaaagg gatgatgacg
    28321 gtcatttatg aattcccggt ggattctgaa actaccctgc aaaactacga tatttacttc
    28381 accaatgaag agttaaccga cgagcaaaaa tcgctgattg agtggtatcg cgatgtgttc
    28441 cgtccggaag atttacgtct ggttgaaagc gtacagaaag ggctgaaatc gcgtggctat
    28501 cgtggtcagg ggcgcatcat ggccgacagt agcggtagtg gcatttccga acatggtatc
    28561 gcccatttcc ataatctgct ggcgcaggtg tttaaggact aatgacatcg gcggcggtat
    28621 tttccgccgc tgggctgatt tttgatggag tacagcaatg tcagactatc aaatgtttga
    28681 agtacaggtg agccaggttg aaccccttac cgaacaggtg aaacgcttca cgctggtggc
    28741 aaccgatggc aaaccattac ctgcgtttac cggaggaagt cacgtcattg tgcagatgag
    28801 cgatggtgat aaccagtaca gcaatgcgta ttcactactg agttcgccgc atgacacctc
    28861 ttgttatcag attgccgttc ggctggagga aaactcgcgc ggcggttccc gctttttgca
    28921 tcagcaggta aaagtgggca atcggttaac gatttcaacg cctaataacc tgtttgcgca
    28981 aattccctca gccagaaagc atctgtttat cgcgggcggt attggtatca cccctttcct
    29041 gtcgcacatg gcagagcagc aacacagcga cgtcgactgg cagctacatt actgctcgcg
    29101 aaatccagaa agttgcgcat ttcgtgatga gctagtccag catccgcagg ctgagaaagt
    29161 tcacttgcat cactcatcaa ccggaacacg actggaatta gcgcgattat tggcggatat
    29221 cgaacctggc acacacgttt atacctgtgg tcccgaggcg ctaaatgaag cggtaagaag
    29281 tgaagctgcg cgtctggaca tcgccgccga tacgctgcac tttgagcaat ttgctatcga
    29341 agacaaaacc ggcgatgcct ttaccctggt gcttgcccgt tccggaaaag agtttgtggt
    29401 gccggaagag atgactattt tgcaggttat tgaaaataat aaagccgcga aagtggaatg
    29461 tttatgtcgt gaaggggtat gcggaacctg cgaaacagca atactggaag gtgaagctga
    29521 ccatcgggat cagtatttta gcgatgaaga gcgtgccagc cagcaaagta tgttgatctg
    29581 ttgttcgcgt gcgaagggta aacgcctggt gttggatttg tagtttgcca aagccggatg
    29641 tggcgctgag cgcgcccagt ccggcttcgg aagattttac tgcggatatt cctgcaataa
    29701 attgtgtaat gcttccgcca tcagctcacc acgccagccg gaaatcagct ccggcaaatt
    29761 gttctgcggt ttcagtttcc agtgccagtt cagcagttgg ttgatttgcc gacgcgatgc
    29821 cagcaattcg gcgctgatct tatgcgtttc gctcacgtca gtaatcagcg acttaatcgc
    29881 tttaaacgct ttacgataac caggcatgtc catcaggtta agcatcggct gcggtaaggc
    29941 ctcttccggc aatgtctgcg ctttttccac cagcgccagc agcgttttac cgtgaaagcg
    30001 gatttcgcta ccggataaac ccaggctgtc cagttcgcct aaacttcccg gcatataacg
    30061 cgcgaccgac cacaaatgct cttcacgcac gacaaagttc accgccagat cacgctctcg
    30121 cgccttgcgc agtcgccagt cggctaacag ttgcagacag gccagttggc gtgtgcgtaa
    30181 ctgccaggca ttggtgatat cacgccaggc atcttccggc gcaacgactt cctgacgacg
    30241 catttgcatc aggcggcatt catccagcgc cgcaggtagc cagccggagg cctccgtttc
    30301 taccataagc ttggcggtga tcggtaacag ataccagaca tccgccgctg cgtattcaca
    30361 ctgacgttcg gtcagcggtc tggccagcca gtcggtgcgc gattcactct tgtccagcgt
    30421 aacgccggaa tactcttcca ccatggaagc gaaaccccat gacatcgggc gtccgcagaa
    30481 ggcagcaagg atttgcgtgt caatcaaggg ttgtggtaat tcgccaaaga cattgaggaa
    30541 cacttccaga tcttcactgc ctgcatggag aaattttgtg atggacggat cgcgcaggat
    30601 cgctttcagc ggtgaccagt cggtgatccc gagaggatca attagcgcca gatgctcgcc
    30661 atcgaaaagt tgaatcaacc ccagctgcgg gtaataagtg cgcgtacgaa caaattcagt
    30721 atccagggct atcgccggaa aggcacggac ggcttcacac aaagaagcca gcgcatcgtc
    30781 cgtggtaatc atttggtaat tcaaattgtt ttctctttag tgggcgtcaa aaaaaacgcc
    30841 ggattaaccg gcgtctgacg actgacttaa cgctcaggct ttattgtcca ctttgccgcg
    30901 cgcttcgtca cgtaattctc gtcgcaaaat ttttccgacg ttagatttcg gtaactcatc
    30961 acgaaactcc accagcttcg gtactttgta tcccgtgagc tgacggcggc aaaaagtcac
    31021 cagtgactct tcggtaagcg atggatcttt tttcactacg aagattttca ccgcttcacc
    31081 actggagccg gaaggtacgc caacagccgc gacttcctgt acgccaggat gctgcatgac
    31141 gacatcttca atctcgttgg gatagacgtt aaaaccggaa accagaatca tgtctttttt
    31201 acgatcgaca atgcgcagga atccttcttc atccattacc gcgatgtcgc cggtgtgtaa
    31261 ccagccattt ttgatgattt catcggtagc atcgggacgc tgccagtaac ccagcatcac
    31321 ctgcggtcct ttgacacaaa gctcacccgg ttgacctggt ggtacttcat tatcatcatc
    31381 atccaccagt ttggcttccg tcgacggcac cggcaaaccg atgctaccac tatgataatc
    31441 aatatcatat gggttaacgc tgaccagcgg cgcacactcg gtaaggccat agccttccag
    31501 cagatactgt ccggtcagtt tcacccaacg ctctgccacc acttgctgca ctggcatccc
    31561 accgcctgcg gaaagatgca gactggagaa atccagctgc tggaactctt tattgttcag
    31621 caacgcattg aacaaggtgt taacgccagt gatagcggta aacggatatt tcgctaactc
    31681 ttttaccaac cctggaatat cacgcgggtt agtgataagc aggttctgcc cacccagttc
    31741 gataaacagc aggcagttaa tggtcagggc aaaaatgtga tacagcggca gtgccgttac
    31801 taccagttct ttacctggat gcagcaacgg cccataggtg gcgttgacct gttccaggtt
    31861 cgccagcata ttgcggtgag tcagcatcgc gcctttcgcc acacctgtgg tgccgccggt
    31921 gtattgcagg aaggctaaat cttccggcac cagttcgggt ttgacgtact gcatccggta
    31981 gccgttatgc agtgcgctac gaaatgaaat ggcatctggc agatggtatt tcggcaccaa
    32041 acgcttgatg tatttaacaa cgaaattgac taccgtgcct tttgccgtag atagctgatc
    32101 gcccatacgg gtcagaatta cgtgctgaac ggcggtttta tcaaccactt tttccagtgt
    32161 gtgagcaaag ttagacacga taacaatcgc cgatgcgccg ctatcgttaa gctgatgctc
    32221 aagctcacgc ggggtataca acgggttaac gtttacgacg atcatcccgg cacgcaaaat
    32281 gccaaacagc gccaccggat attgcaataa attaggcatc atcaacgcaa cgcgatcgcc
    32341 tttcttcagc cccaaccctt gttgcaaata agcggcaaac gcgcgactgc gttcttccag
    32401 cttgcggaag gtcattacct cccccatatt cacaaacgca ggctgatcgg cgtagcgcgc
    32461 gaccgactgc tcaaacatat ctaccagaga ttgataacgg tcagggttga tctccgtcgg
    32521 aacgtccgcg ggataacggt taagccaaac cttcttcaat tcttcacctc taaaatgcgt
    32581 gttcgtcgtc atcgcaaccc caaatgatat acatgccgtt aacataatat taactcatca
    32641 taccagcttg ataattaccc aacgaaaagg ttgcgaagcg cgtcactatt tatttttatc
    32701 tttaccgtaa gaatgcagaa acagcggacc agccgctgtt tctttttctt taaaaacaag
    32761 caattattca gttacaactg tttgtactct cgcggggccg ggattatacc agccccatcc
    32821 gccgtagcca tagggccagc cacgaccgcc ataaaaccat ggatcaatcg gctgaggcgg
    32881 cataatcacc tgctgggtta aatgccaacg tttgtaaccc gttacttgca tcaccataaa
    32941 tttatagggc gtattgccga ttttgccgtc aaccgcacca gtgattggcc cgactaccgt
    33001 aaccagttgt ccacggaaat ccaccgggtc cagaaaaccg ttcacatcgg cataaatgcg
    33061 accgcgagaa ggttctccca gcgtcggtct ggctccgctg tccagcggta cggtagcaat
    33121 ttccaggcgg gttttccctt gctggttttg taccgcaacc actttgccac caaagcgtgc
    33181 ctcctgacca acgtacagct gcggcgcact catcacccga actaaatctt gttgcggcgt
    33241 gggactgctg cctttaatgg cgtccggcac agtgacacaa ccgctcagca ttagcgcaaa
    33301 cgtacctgcc agtatgcctt tgataacatt cttttgaacc gccatggtgc gactcctttt
    33361 tttctcaggg catactctta agattcattc tttgcccgga agtttcttcc atgcgacgtt
    33421 gttacgtaaa taaaccggtt cggcatgttc caccgccacc gttttaccct cagcaaacat
    33481 ctgacacgca atcggcagca tatcttcagc agcaggcagt aacacttcgc catcgcgcaa
    33541 aaccagcccg ctctctttac cgagatccgg ccaagcttgc cagcccgtcc ctaccgtcac
    33601 ccattcgccg gaaagctgtt gcattcgttc atggacgatt tcgggtttga gtacggcttc
    33661 ggtttcttca ccgtgccaga taccgttttc atcacgctga tattcggccc agtaaacttc
    33721 gcccattcgc gcgtcaatgg ctgccagcac gcgggttgcg ccgtttttgc gccacgcccc
    33781 ttgcgccatc gtcattagcg tggagacgcc aatcatcggt aattccgcgc caagcgccag
    33841 cccttgcgcg atgccaatac caatgcgcac gccagtaaag ctaccggggc cgcgcccgta
    33901 agccagagcg ttaatatcag tcagggaagt tccgctggtg gtcaggatat cctgcaccat
    33961 cggtaagatt cgttgagtat gttcacgagg gcaaagctca aaatgagcgt tgacagtacc
    34021 gtcgttccac agggcgacag agcaggcctc tgtcgcggta tcgatagcca gaattcgcat
    34081 gggtcttcgt gcttagatca ataaaaaggc gcgcatcata ccatactccg taacaaatta
    34141 cctggaggat ggtatcgcaa ggaaacgaac cgcacgggca atgtcacggg tgcgtggcgc
    34201 gggcggcaga ctggcgagaa acgtcgcgcc gtaaggacgc atcaccagcc gattgtcaca
    34261 aatcaccagc acgccacgat cgtcggcgtc gcgaatcagt cgccctaccc cctgtttgag
    34321 agtaatgacg gcatctggta gttgcacttc atcgaacggg tcgccaccgc gcaaacgaca
    34381 atcttccatg cgcgctttta acagtggatc atccggcgag gtaaacggca atttgtcgat
    34441 aattaccaat gacaatgtat cgccacgcac gtccacccct tcccagaaac tgctggttgc
    34501 cacaagaagc gcattaccgg cgctgacaaa ttgctgcaac agttgccctt tgctggtttc
    34561 cccctgcaac aatacaggaa gcgtcatggt agcgcggaac tgctcggcca gatcgcgcat
    34621 catggcgtgc gaggtacaaa gcataaaaca acgaccgttg ttagcttcga tgatcggtcg
    34681 cagcattgcc gctaactggc gagcagaacc tggctggttg gtttgcggca gattgcgcgg
    34741 cacacagagt aacgcctggc ggctgtaatc aaatgggctg ggcaacagca acgactcggc
    34801 ctgttcgatg ccaagccgcg aggtgaaatg atgcagatcg tcgttcaccg acagcgttgc
    34861 tgaggtgaag atccagctac cgggtttttg cgccattaac tctttgaatt tatccgccac
    34921 gctgagcggc gtgagagcca gagtaaaatg gcgcgaagtg cattcgtacc agtagctgta
    34981 gcccggctga ttgatctctt ttagccgctt cagccgtgtg cgatacaacg tagcgcgctc
    35041 aaatgccgca tccagcaagg cggaacgccc cagtgacagt ttcgccacgt cataacaaag
    35101 ttccagggtg tcatcgagca gtaaaaatgc ccgctgaatt tgcggattag ctaacagctc
    35161 acgcaggtta ccgcgataac ctggctcacc gagttgcaga cgaaaatcct gcgcactctg
    35221 ggcaagacga tcagcgcact tttgtaactg ctgggtgtct tttaattcgg tgcggtaggc
    35281 gatggtgatg tcttttgcca ggtcgagcag ttgtcgactg gagagtgact gaccaaaata
    35341 ctggctggca atgtccggta gctggtgggc ttcgtcgaag atcatgacgt ccgcttccgg
    35401 gatcaactcg ccaaatccac tctctttaac caccatatcc gccagaaaga gatgatggtt
    35461 taccaccacc acatcggcgt ccatcgcttt tttacgtgct ttgaccacaa agcaatcttt
    35521 atacatcggg cagtcgctgc caagacagtt gtcgttggtg ctggtgacca gcggccacgc
    35581 ctgtgaatct tccgccacgc tgacgcaggt gctgatatca ccatcgactg tttgattaga
    35641 ccaggagcgc agcaggatca catcgcttaa gatttgtacc ggcagatcgc cccccgccag
    35701 cgcctgctgt tcgagacgtt cgaggcagag gtagtttgag cgccctttca gcagcgccac
    35761 gttgcccgta tatttcaatg cctttgagac tgttggcaaa tcgcggctgt agagctgatc
    35821 ctgcaacgct tttgagccgg tcgagataat gactttcttt ttcgcccgca gcgcaggagc
    35881 caggtaagcg taggttttgc ccgtaccggt tcctgcttcc accaccagcg gctggccttt
    35941 ttctatcgcc tgggtgacgg ctaccgccat ctgtcgctgt ggttctcgcg gcttaaagcc
    36001 tggtatcgct ttcgccagct gaccgtctgg tgcaaaatcg tccgtcacac taccccctgt
    36061 tgatttgaac agggattatg tcaggatgag ggcgctttcg ccagttgaag tggtgacggc
    36121 gacctcacat tgtggcagtc tttgcagcac gaaaatggaa gtataatgag gacaaaatga
    36181 tgactatcgt tcgtatcgat gctgaagccc gctggtctga tgtagtaatc cacaacaaca
    36241 cgctctacta cactggtgta ccggaaaacc tcgacgccga tgcctttgag caaaccgcca
    36301 acacgctggc acagattgac gccgtgctgg aaaaacaggg cagcaataaa tcgagcattc
    36361 tggatgccac cattttcctg gccgataaaa acgacttcgc ggcgatgaat aaagcgtggg
    36421 atgcctgggt tgtggcgggt catgcgccgg tgcgctgcac ggtgcaagcg gggctgatga
    36481 acccgaagta taaagttgaa attaagattg tggctgcggt gtaagcttta tcgaagcaaa
    36541 ataagtcaga cgataattta tcgataatac tggtcggttt tacataaatc gaccagagat
    36601 taagatatta ctgttgatgc tcctgctaat tcattattta tttttataat ttcaatttta
    36661 gctataaagc taactatata aataaacata accatgccgt tgtgttcgcc cttcgattag
    36721 cacctctggt tttctttctt agctgaacaa tctggttata gcgtgcggac tgaccgttaa
    36781 ttttctccat cttactggat aacacaccat gagctgctac ctttaaaaca cctgaatgtc
    36841 aacaggttaa ctcgtgccat atcgttataa tccctttgat attagatgca aattaaggtc
    36901 atatatagcc ttatcgataa catggttaat tttaaggata agaatatgcc tgctgtaata
    36961 gataaagccc tggatttcat tggtgccatg gatgtatcag cgccaacacc aagttcgatg
    37021 aatgaaagca cagcgaaggg aatctttaaa tatttaaaag aactgggagt acccgcgagt
    37081 gccgctgata ttacggcgcg agctgaccag gaaggctgga accctgggtt cacggaaaaa
    37141 atggttggat gggcaaaaaa aatggagtct ggtgaacgca ctgtaattaa aaatcctgaa
    37201 tacttttcaa catatatgca ggaagaacta aaagcactgg tctgagttta atttatatca
    37261 gcataaatgg gtcaggacgc ttttaatcac atataaaaag cgtccttttt ctcacaatca
    37321 aataatcatc ggattctata acgaaatcga tacggattag tgtgttactc gtcttcatcc
    37381 tcaaaacgcg ccacgattcg ctcgccggag tggttggcac gcagctcttc tgccaccagc
    37441 gcgattgcct ggccgctgct catcccttgt gccatcagtt cctggatccg ctcgacagct
    37501 ttttgctgct gttcatgggt gagtgaaggt aaacctgcaa acattgttaa ctcctgctaa
    37561 attgttggcg ctaattattt catgctaccc ggcacatagc cagtagagtc aggactgatg
    37621 aagacgttat ctcccgctgt gattacttta ccctggcgtc aggacgccgc tgaattttat
    37681 ttctcccgct taagccacct gccgtgggcg atgcttttac actccggcta tgccgatcat
    37741 ccgtatagcc gctttgatat tgtggtcgcc gatccgattt gcactttaac cactttcggt
    37801 aaagaaaccg ttgttagtga aagcgaaaaa cgcacaacga ccactgatga cccgctacag
    37861 gtgctccagc aggtgctgga tcgcgcagac attcgcccaa cgcataacga agatttgcca
    37921 tttcagggcg gcgcactggg gttgtttggc tacgatctgg gccgccgttt tgagtcactg
    37981 ccagaaattg cggaacaaga tatcgttctg ccggatatgg cagtgggtat ctacgattgg
    38041 gcgctcattg tcgaccacca gcgtcataca gtttctttgc tgagtcataa tgatgtcaat
    38101 gcccgtcggg cctggctgga aagccagcaa ttctcgccgc aggaagattt cacgctcact
    38161 tccgactggc aatccaatat gacccgcgag cagtacggcg aaaaatttcg cctggtacag
    38221 gaatatctgc acagcggtga ttgctatcag gtgaatctcg cccagcgttt tcatgcgacc
    38281 tattctggcg atgaatggca ggcattcctt cagcttaatc aggccaaccg cgcgccattt
    38341 agcgcttttt tacgtcttga acagggtgca attttaagcc tttcgccaga gcggtttatt
    38401 ctttgtgata atagtgaaat ccagacccgc ccgattaaag gcacgctacc acgcctgccc
    38461 gatcctcagg aagatagcaa acaagcagag aaactggcga actcagcgaa agatcgtgcc
    38521 gaaaatctga tgattgtcga tttaatgcgt aatgatatcg gtcgtgttgc cgtagccggt
    38581 tcggtaaaag taccagagct cttcgtggtg gaacccttcc ctgccgtgca tcatctggtc
    38641 agcactataa cggcgcgact accagaacag ttacacgcca gcgatctgct gcgcgcagct
    38701 tttcctggtg gctcaataac cggggctccg aaagtacggg ctatggaaat tatcgacgaa
    38761 ctggaaccgc agcgacgcaa tgcctggtgc ggcagcattg gctatttgag cttttgcggc
    38821 aacatggata ccagtattac tatccgcacg ctgactgcca ttaacggaca aattttctgc
    38881 tctgcgggcg gtggaattgt cgccgatagc caggaagaag cggaatatca ggaaactttt
    38941 gataaagtta atcgtatcct gaagcaactg gagaagtaag acgtggaata ccgtagcctg
    39001 acgcttgatg attttttatc gcgctttcaa cttttgcgcc cgcaaattaa ccgggaaacc
    39061 ctaaatcatc gtcaggctgc tgtgttaatc cccatcgtcc gtcgaccgca accggggttg
    39121 ttgctgactc agcgttcgat tcatctgcgt aaacacgctg gacaagtggc attccctgga
    39181 ggtgcagtcg atgacacgga cgcatcagct atcgccgccg cgctgcgcga agctgaagaa
    39241 gaggtcgcta taccgccttc cgccgttgaa gttatcggcg tgctgccgcc cgtcgatagc
    39301 gtcactggct accaggtaac cccagtggtc ggcattatcc cgcccgatct gccgtatcgc
    39361 gccagtgaag atgaagtctc ggcggtgttt gaaatgccgc tcgcccaggc attacatctg
    39421 ggtcgttatc accctttaga tatctaccgc cgtggtgatt cacatcgggt atggctgtcc
    39481 tggtacgaac agtattttgt atggggaatg accgcaggca taattcgtga gctggcgctg
    39541 caaattggtg tgaaaccctg actatactta tctttacatc tacaaaacac tacttgagac
    39601 aatcatcgca atattagtta aatcgcggtt tttgattagt ttaattcatg tgaatagtta
    39661 agccagtcgc cgcgttccct cttacactat gcgctgttat tagttcgtta ctggaagtcc
    39721 agtcaccttg tcaggagtat tatcgtgatt agtctattcg acatgtttaa ggtggggatt
    39781 ggtccctcat cttcccatac cgtagggcct atgaaggcag gtaaacagtt cgtcgatgat
    39841 ctggtcgaaa aaggcttact ggatagcgtt actcgcgttg ccgtggacgt ttatggttca
    39901 ctgtcgctga cgggtaaagg ccaccacacc gatatcgcca ttattatggg tcttgcaggt
    39961 aacgaacctg ccaccgtgga tatcgacagt attcccggtt ttattcgcga cgtagaagag
    40021 cgcgaacgtc tgctgctggc acagggacgg catgaagtgg atttcccgcg cgacaacggg
    40081 atgcgttttc ataacggcaa cctgccgctg catgaaaacg gtatgcaaat ccacgcctat
    40141 aacggcgatg aagtcgtcta cagcaaaact tattattcca tcggcggcgg ttttatcgtc
    40201 gatgaagaac actttggtca ggatgctgcc aacgaagtga gcgtgccgta tccgttcaaa
    40261 tctgccaccg aactgctcgc gtactgtaat gaaaccggct attcgctgtc tggtctcgct
    40321 atgcagaacg aactggcgct gcacagcaag aaagagatcg acgagtattt cgcgcatgtc
    40381 tggcaaacca tgcaggcatg tatcgatcgc gggatgaaca ccgaaggtgt actgccaggc
    40441 ccgctgcgcg tgccacgtcg tgcgtctgcc ctgcgccgga tgctggtttc cagcgataaa
    40501 ctgtctaacg atccgatgaa tgtcattgac tgggtaaaca tgtttgcgct ggcagttaac
    40561 gaagaaaacg ccgccggtgg tcgtgtggta actgcgccaa ccaacggtgc ctgcggtatc
    40621 gttccggcag tgctggctta ctatgaccac tttattgaat cggtcagccc ggacatctat
    40681 acccgttact ttatggcagc gggcgcgatt ggtgcattgt ataaaatgaa cgcctctatt
    40741 tccggtgcgg aagttggttg ccagggcgaa gtgggtgttg cctgttcaat ggctgctgcg
    40801 ggtcttgcag aactgctggg cggtagcccg gaacaggttt gcgtggcggc ggaaattggc
    40861 atggaacaca accttggttt aacctgcgac ccggttgcag ggcaggttca ggtgccgtgc
    40921 attgagcgta atgccattgc ctctgtgaag gcgattaacg ccgcgcggat ggctctgcgc
    40981 cgcaccagtg caccgcgcgt ctcgctggat aaggtcatcg aaacgatgta cgaaaccggt
    41041 aaggacatga acgccaaata ccgcgaaacc tcacgcggtg gtctggcaat caaagtccag
    41101 tgtgactaat acttcttact cgcccatctg caacggatgg gcgaatttat acccgctttc
    41161 tcgtctgctg taatattccc cactacactt ccactgttgc gtcaggcgtt tgtcgccata
    41221 cgcttacagg gtggcccgca tgcaaaaagc acaacggatc attaaaacct atcgtcgtaa
    41281 tcgaatgatt gtttgtacga tttgcgcact cgttacgctc gcttcgaccc tgagcgtgcg
    41341 atttatttca cagcgtaact taaatcaaca acgggtagta caattcgcca atcacgctgt
    41401 agaggaatta gataaagtac tgcttcccct acaggcaggt agcgaagtct tgcttccgct
    41461 gattggtctg ccctgctctg tcgcccattt gccattacgt aaacaggcgg caaaactcca
    41521 aactgtgcga tccattggcc tggtgcaaga cggcacactt tattgctcca gcatttttgg
    41581 ttatcgcaat gtgcccgtcg tggacattct ggctgaactt cctgcaccgc aaccactttt
    41641 acgcctgacg atcgaccgtg ccctgattaa aggcagtccg gttttgattc aatggacgcc
    41701 tgcagcgggc agtagcaatg ctggggtcat ggagatgatt aacatcgact tactgacggc
    41761 aatgctcctt gagccacaac tgccgcaaat cagtagcgcc agcctgacgg tggacaaacg
    41821 gcatttgctc tatggtaatg ggctggtaga ttcccttccg caacctgaag acaatgaaaa
    41881 ctatcaggtt tcttcgcaac gctttccttt taccattaac gttaatggtc cgggggctac
    41941 ggcgctggca tggcactatc ttccaacaca attaccgctg gcggtgctgc taagtttact
    42001 ggtgggctac atcgcctggc tggcgaccgc ttaccggatg agcttttccc gcgaaatcaa
    42061 tctgggcctg gcgcaacatg agttcgaatt gttctgtcag cctttgctta atgcgcgcag
    42121 ccagcaatgt attggtgtag agattttgct gcgctggaac aatccgcgtc agggctggat
    42181 ttcaccggat gtgtttattc ctatcgcgga agaacatcat ttaattgtgc cactgacccg
    42241 ctatgtgatg gcagaaacca ttcgtcagcg ccatgttttc ccgatgagta gtcagtttca
    42301 tgttggcatt aacgtcgcac ccagccattt tcgccgtggt gtgctgataa aagatctcaa
    42361 tcagtactgg tttagcgctc acccgattca gcaactgatc ctcgaaatca ccgaacgcga
    42421 tgccttactg gatgttgatt atcggattgc ccgcgagcta catcgtaaaa acgtcaaact
    42481 ggcgattgat gacttcggca ccggcaacag ttcattttcc tggcttgaaa cattacgtcc
    42541 tgacgtgctg aaaattgata agtcatttac cgcagctata ggttctgacg cggttaactc
    42601 gacggtgacc gatatcatca tcgcgctggg gcaaagactg aatattgaac tggtggcgga
    42661 gggcgtggaa acgcaagaac aggcgaagta tttgcgccgt catggcgtgc atattttgca
    42721 agggtatttg tacgcacagc cgatgccgct acgtgatttt cccaaatggc tggcgggcag
    42781 ccaaccgccg cccgcccggc ataatggaca tatcacgccc attatgccgt tacgttaatg
    42841 cttactcatc ttcatcgtgc gccggttgct ctttaacaat gcgaaccaga tcaacacgat
    42901 aatcattggc ttcaatgatg gtgatatgca gtggccctac atcaatcaca tcgcccacac
    42961 ggggaatgtg accatttgcc gagatcacga ggcccgcgac cgtcgcgata tcatcgtcat
    43021 cggcaaggtg ctcaacatca agcgcctgct gcaaggcatg caaatctgta ccgcctttta
    43081 ccagccagcc gtcaccatca gtaatgattt ccggcgtttc gtcagcgtcc gggaattcac
    43141 ccgcaatggc ttccagcaca tccagcggcg tgaccagacc ttgtaccaca ccaaactcgt
    43201 tggtcacgat aacaaagctc ccgcgagcac gacgcagcac gcccaacagg ttgatcggat
    43261 cgagggtttc cgggacgata atcgccggag acgccgaagc aatcgccgcc acatcaacgc
    43321 cctcttccag cgccaccagc agttctttag cacgtacaat accgatgatt tcatccagtt
    43381 caccgcgaca taccgggaac agactgtgcg gtgaagagag cagttgctcg cggatttcat
    43441 cgaccccgag attagcgtca acccagctta tttcaccgcg cggcgtcatg atcccgcgca
    43501 gagaacgcga cgccagcgtc agtacgccgt taatcatgta acgttcttct tcggcaaatg
    43561 caccttccgg gatcggcatc ggcatcgggt tatcggcatc gtgctgaaca ttggcctgac
    43621 gtttcccgcc catcaaacgc aggatggcat cggcagtacg cgctcgcagc ggcaaagtcg
    43681 actggtggcg aataaagttg cgacgcgcaa tctggttaaa cacttcgatg atgatcgaga
    43741 agccaatcgc ggcatacagg taacctttcg gaatgtggaa accgaaacct tctgccacca
    43801 gactcagacc aatcattaac aggaagctca gacagagcac caccaccgtg gggtgctggt
    43861 taacgaatcg cgtcagcggt ttggatgcca gcaacataac cgccatcgca atcactaccg
    43921 ccgccatcat caccggcaga tggttaacca tccctactgc agtaattacc gcatccaacg
    43981 agaagacggc atcaaggatg acaatctgtg tgacgaccac ccagaaactg gcgtagcctt
    44041 taccgtggcc ggaatcatga tcgcggtttt ccagccgttc atgcagttcg gttgttgctt
    44101 tgaacagcaa gaatatcccc ccgaacaaca taatcaggtc gcgtccggag aaggagaaat
    44161 ccatgacggt aaatagcggt ttggtcagcg tgaccatcca tgaaatcagc gacagcagcc
    44221 ccagacgcat aatcagcgcc agtgataacc ccagcaaacg cgctttatcg cgttgttttg
    44281 gcggcagttt gtcagcaaga atggcgatga agaccaggtt atcgataccc agcacaattt
    44341 cgagaacaac aagcgtgagt agccccgccc aaattgaggg gtccattaag aattccatga
    44401 caagctcctg cttaaggaat agctattcga cgccagaaat aatgcaggcg taacgacaaa
    44461 atgcaaacga aaggtgcggc atagagtgcc agaaaggcag gcgttaaaag gcctgatgct
    44521 gaaatgacgt cggtgacgat ccatactgcg ggctactgcc ctatactcca tggttgttaa
    44581 acgggagtta aacatatcag agacgcctct gatttggcaa agatttacct tcctttgcaa
    44641 acgaatgtga caaggatatt ttacctttcg aaatttctgc taatcgaaag ttaaattacg
    44701 gatcttcatc acataaaata attttttcga tatctaaaat aaatcgcgaa acgcaggggt
    44761 ttttggttgt agcccttatc tgaatcgatt cgattgtgga cgacgattca aaaatacatc
    44821 tggcacgttg aggtgttaac gataataaag gaggtagcaa gtgaccattg ctattgttat
    44881 aggcacacat ggttgggctg cagagcagtt gcttaaaacg gcagaaatgc tgttaggcga
    44941 gcaggaaaac gtcggctgga tcgatttcgt tccaggtgaa aatgccgaaa cgctgattga
    45001 aaagtacaac gctcagttgg caaaactcga caccactaaa ggcgtgctgt ttctcgttga
    45061 tacatgggga ggcagcccgt tcaatgctgc cagccgcatt gtcgtcgaca aagagcatta
    45121 tgaagtcatt gcaggcgtta acattccaat gctcgtggaa acgttaatgg cccgtgatga
    45181 tgacccaagc tttgatgaac tggtggcact ggcagtagaa acaggccgtg aaggcgtgaa
    45241 agcactgaaa gccaaaccgg ttgaaaaagc cgcgccagca cccgctgccg cagcaccaaa
    45301 agcggctcca actccggcaa aaccaatggg gccaaacgac tacatggtta ttggccttgc
    45361 gcgtatcgac gaccgtctga ttcacggtca ggtcgccacc cgctggacca aagaaaccaa
    45421 tgtctcccgt attattgttg ttagtgatga agtggctgcg gataccgttc gtaagacact
    45481 gctcacccag gttgcacctc cgggcgtaac agcacacgta gttgatgttg ccaaaatgat
    45541 tcgcgtctac aacaacccga aatatgctgg cgaacgcgta atgctgttat ttaccaaccc
    45601 aacagatgta gagcgtctcg ttgaaggcgg cgtgaaaatc acctctgtta acgtcggtgg
    45661 tatggcattc cgtcagggta aaacccaggt gaataacgcg gtttcggttg atgaaaaaga
    45721 tatcgaggcg ttcaagaaac tgaatgcgcg cggtattgag ctggaagtcc gtaaggtttc
    45781 caccgatccg aaactgaaaa tgatggatct gatcagcaaa atcgataagt aacgtattgt
    45841 gttgattatc actcagtttt cacacttaag tcttacgtaa acaggagaag tacaatggag
    45901 attaccactc ttcaaattgt gctggtattt atcgtagcct gtatcgcagg tatgggatca
    45961 atcctcgatg aatttcagtt tcaccgtccg ctaatcgcgt gtaccctggt gggtatcgtt
    46021 cttggggata tgaaaaccgg tattattatc ggtggtacgc tggaaatgat cgcgctgggc
    46081 tggatgaaca tcggtgctgc agttgcgcct gacgccgctc tggcttctat catttctacc
    46141 attctggtta tcgcaggtca tcagagcatt ggtgcaggta tcgcactggc aatccctctg
    46201 gccgctgcgg gccaggtact gaccatcatc gttcgtacta ttaccgttgc tttccagcac
    46261 gctgcggata aggctgctga taacggcaac ctgacagcga tttcctggat ccacgtttct
    46321 tctctgttcc tgcaagcaat gcgtgtggct attccggccg tcatcgttgc gctgtctgtt
    46381 ggtaccagcg aagtacagaa catgctgaat gcgattccag aagtggtgac caatggtctg
    46441 aatatcgccg gtggcatgat cgtggtggtt ggttatgcga tggttatcaa catgatgcgt
    46501 gctggctacc tgatgccgtt cttctacctc ggcttcgtaa ccgcagcatt caccaacttt
    46561 aacctggttg ctctgggtgt gattggtact gttatggcag tgctctacat ccaacttagc
    46621 ccgaaataca accgcgtagc cggtgcgcct gctcaggcag ctggtaacaa cgatctcgat
    46681 aacgaactgg actaacaggt gagcgaaatg gttgatacaa ctcaaactac caccgagaaa
    46741 aaactcactc aaagtgatat tcgtggcgtc ttcctgcgtt ctaacctctt ccagggttca
    46801 tggaacttcg aacgtatgca ggcactgggt ttctgcttct ctatggtacc ggcaattcgt
    46861 cgcctctacc ctgagaacaa cgaagctcgt aaacaagcta ttcgccgtca cctggagttc
    46921 tttaacaccc agccgttcgt ggctgcgccg attctcggcg taaccctggc gctggaagaa
    46981 cagcgtgcta atggcgcaga gatcgacgac ggtgctatca acggtatcaa agtcggtttg
    47041 atggggccgc tggctggtgt aggcgacccg atcttctggg gaaccgtacg tccggtattt
    47101 gcagcactgg gtgccggtat cgcgatgagc ggcagcctgt taggtccgct gctgttcttc
    47161 atcctgttta acctggtgcg tctggcaacc cgttactacg gcgtagcgta tggttactcc
    47221 aaaggtatcg atatcgttaa agatatgggt ggtggcttcc tgcaaaaact gacggaaggg
    47281 gcgtctatcc tcggcctgtt tgtcatgggg gcattggtta acaagtggac acatgtcaac
    47341 atcccgctgg ttgtctctcg cattactgac cagacgggca aagaacacgt tactactgtc
    47401 cagactattc tggaccagtt aatgccaggc ctggtaccac tgctgctgac ctttgcttgt
    47461 atgtggctac tgcgcaaaaa agttaacccg ctgtggatca tcgttggctt cttcgtcatc
    47521 ggtatcgctg gttacgcttg cggcctgctg ggactgtaag actgttgtac actaccgggg
    47581 ccttttggcc ccgttttttt atctggagga ttaatgacaa tcacggacct ggtactgatt
    47641 cttttcatcg ccgcactcct ggccttcgcg atctacgatc agttcatcat gccccgccgt
    47701 aatggcccca ccctgctggc aattcctttg ctccggcgtg gtcgcatcga tagcgttatc
    47761 ttcgtcggat tgattgtcat tcttatctat aacaacgtca cgaatcatgg tgcgttaata
    47821 acgacatggt tattaagcgc actggctctg atgggttttt atatattctg gatccgcgtt
    47881 ccgaagatca tctttaaaca aaaaggtttt ttcttcgcca atgtctggat tgaatatagc
    47941 cgaatcaaat cgatgaactt gtcggaagat ggcgtgttgg tgatgcaatt agaacagcgt
    48001 cggctgttaa tccgcgttcg aaatatcgac gatctggaaa aaatttataa gcttctcgtt
    48061 tcaactcaat aagttatgaa tttagccaaa gctatgttta gtgtattttt aataatcaga
    48121 catagcttag gctatattac ctcttccctt atttgttatt tattttaacg tttcattgat
    48181 atataaatct aaatgtaaat cgttatcaat aaagcaatga aataatatat tccaacagtt
    48241 gttttatatt ctcaaaatat gttaaggttg cgccctcatt tggggagtag ccgatttcca
    48301 gattccggaa atgtacgtgt caacatactc gttgcaaaac gtggcacgta cggactgaat
    48361 actttcagtc aggcgagacc atatgcacat caatcgctat gcctgcatga acgcgaaaac
    48421 catcgcggct tgttgttcat gtatgcatgt ttgctggggg cgatgatgtg ttttatggat
    48481 accccggtca ggacattgtc atgaatatca ctgctactgt tcttcttgcg tttggtatgt
    48541 cgatggatgc atttgctgca tcaatcggta aaggtgccac cctccataaa ccgaaatttt
    48601 ctgaagcatt gcgaaccggc cttatttttg gtgccgtcga aaccctgacg ccgctgatcg
    48661 gctggggaat gggcatgtta gccagccggt ttgtccttga atggaaccac tggattgcgt
    48721 ttgtgctgct gatattcctc ggcgggcgaa tgattattga gggttttcgt ggcgcagatg
    48781 atgaagatga agagccgcgc cgtcgacacg gtttctggct actggtaacc accgcgattg
    48841 ccaccagcct ggatgccatg gctgtgggtg ttggtcttgc tttcctgcag gtcaacatta
    48901 tcgcgaccgc attggccatt ggttgtgcaa ccttgattat gtcaacatta gggatgatgg
    48961 ttggtcgctt tatcggctca attattggga aaaaagcgga aattctcggc gggctggtac
    49021 tgatcggcat cggcgtccag atcctctgga cgcacttcca cggttaataa gaacgctgcc
    49081 agaggtgaat attaaagtcc gtctggcagt cgaacacttc ttttgctgcc agtgtttgcc
    49141 agacttctgg cttcgcacgc caggcaaacg gcgtcatctg caataatgcg acggcttcat
    49201 caccgcgaag acgcatcgga taacacaact ccgcactctg ctgtaatgta aaaccttcca
    49261 gttgttctgc atgaggtgca tgaagatgta cttcattgta aatcagcccc ttcagctcca
    49321 tcaaatgtcg cggtcccggc gtggcagtaa tgacccagcc gccgggcttc actactcgtg
    49381 ctaattcttc tgctttacac ggcgcgtaaa tacgtattat ggcgtccata ctggtatcgg
    49441 aaaacggcaa acggtggctg gaagcgacac aaaaagtgac ctgcggatag cgtttcgccg
    49501 ccgcttttat cgctaccttc gaaacatcca gaccaaacgt ggtgatttcg ggcaacgcat
    49561 cggcaaatgc gtgtgtgtaa tacccttcac cacagccaat atccagcacc gccgtggcct
    49621 tatcatcaag ccgttccctc agttgggcga caattgcatc acgcagcggc tgataatgtc
    49681 cggcatctaa gaatgcgcgg cgtgcttgca tcatttccgc gctgtcgccc ggatcacgag
    49741 accgtttatg ctgaacgggc agcagattga catacccttc tttcgccata tcaaactgat
    49801 gtcgctgggg acagatatag ctgttttttt cacgcgaaag aggctgatgg caaagtggac
    49861 aggaaaaaga catgacaact ccggcaggat actaaaggcc gcaagtgtaa cgcgaatcac
    49921 gcggcgggca aatatccgaa cgccaggagc gaagataaag catagtaaaa agcctcgcat
    49981 tcgacgaggc tttatagcga gattgaagcg tattcacact tcagatcagt ggattcgatc
    50041 agatagctgt tacgttaaca gctgccggac ctttctggcc gtcctgaatt tcgaactcaa
    50101 cgttctgacc ttcagccaga gttttgaagc cattaccctg gatagcggag aagtgtacga
    50161 acacgtcttt gctgccatca gccggagtaa tgaagccaaa acctttagac tcgttgaacc
    50221 acttaacctg acctttaatc tttgccattt gaaaaattcc ttagattgtt ttcttcgccc
    50281 gcaggcataa catagataaa actgacacat tactgcatga ggcaccaata taaggctcgg
    50341 cagagaagcg gtattcaacg tcaacgtgtt tactcaggac ttctttactg aaaatgccac
    50401 acataaacag aactgtacct cgtttaaccc gaaatctgtt atcacataca acgttaatta
    50461 tggcaagcca tttttaaaca tgtctcgatc agacgcacaa atctcgatac gtcttccact
    50521 ttttttgcac acttatgcaa cggaatacgc gccgatatat cattgtgctt aaccttgcca
    50581 gttcaggcag atacttaaac tggcgtagtt tctaacatag ttcaataagt tcagttgttc
    50641 caggccagtg ggagaagtta ttacatagtg cgtgcaatat cacatttttt gctatgcaat
    50701 gaataaaaag ttatatcact ttttctcata aaacagtcag ttaacggcta tcaattaccc
    50761 taaagagaag tcaatcccca aagggattga aaatttaaaa taagaaaaat tgatgaatga
    50821 gcaaaaaatc aagagagaaa cgtttcgcta ataaataata ccacgcttaa tcacaaatcc
    50881 gatgtaaaca tcctaataaa ttaatgagga aaatatcttt ttctgatatc ccgttacgca
    50941 aataaataat atgataatta tttgaagtca gaccagagtt ttaatttgaa gcagttgcac
    51001 catggcagtg cgtataatca ggatgaattg agtaaccctt caacgacggg gttactcaag
    51061 attgaggaag gattatcgtt cggcgaccag acgaataata tcttcgtctt caaccgattt
    51121 ttgactctca acggtctttt tatctgcttt ctcaggttcg tttgcttcgc acaaacgacg
    51181 taacagtgca ttttgacgtt tttgctgatc cagcaacgcc tcaagcagtt caatctgttc
    51241 gttagtccga gaactggcac gattgataaa aaaccacaag atgagcccga taagaagaac
    51301 caccaccgat acaaccaaag acgcaatgtt catcacgcct gaatttacaa cttcgttcat
    51361 ttcaccacct ctgagtagag gcgctattct accactgctg gagaggaaga aaatctagtg
    51421 ctgaaaaaat gatatcacca cgggataaac tggttaatgg cacaaattcc gctgaaaaat
    51481 tgtacatcct gatcgcacat catgttgaat acctgcgccc aaagcagcaa gcaagccaac
    51541 accacgacaa ccagaacgac ccatcgaaac tttttcactc caatctccgc ttcacctacc
    51601 ttatgtcatt aaactagcat gcgttagtta aacagcgcct aactatgtcg atattcgtgc
    51661 tttttcggaa tgagtagctt gcttcagcgc atattgccgc tacgattaag cgaacataaa
    51721 aagagaagag gttgtaatgc gattcatcat tcgcacagtt atgctgatag cactggtatg
    51781 gataggatta ttactcagcg gatatggcgt tttgattgga agcaaagaga atgccgcagg
    51841 attaggtttg caatgtacct atctgactgc cagaggcacc agcacggtac aatacttgca
    51901 cactaaaagt ggtttcttgg ggataacaga ttgcccactg ttacgtaaaa gcaatatcgt
    51961 ggtcgataat ggttaagggt aataaataac cagattctca ctcatgagta aaatattctt
    52021 atagcaagcg cgtttaatat aacgcacctt caactttatt tttctcatgc taaataaaga
    52081 acctgtctgt ttgatattcc gacaggttct tactctcatc agaacggata gtcgtgataa
    52141 cccatttggg cagaaatttt gcgcgctgcg gtatgcaaca ttgcgacata ttcctgtaaa
    52201 cgctcttcag agaaacgcaa cgtcgggaag gagatgctca aaccggcaat gaccacgcca
    52261 aagcgatcaa ataccggtac cgcaatgcat cgcagccctt cttcctgctc ttcgttatct
    52321 tcgccatacc cctgctcgcg cacctggtcc agaacgggta ataacgcttc tgtactggtg
    52381 atggtccgct cggtactgcg tttatactcc acgccctcaa gaatttgctt cacttcatcg
    52441 cgatcgcgcc atgccagcag taccttacca atcgcggtgc tgtacagcgg attacgacgc
    52501 ccaatccgtg aatacatgcg caaattgtac atagagtcaa ttttgtgaat gtaaacaata
    52561 ctgtcttcgt ccagtgcgcc gaggtggata gtttctttgg tcaggcggga gatctcacgc
    52621 atctggatat ctgcgctacg aattaaatcg acgttttgta acgcgcgagc gcccagttca
    52681 aacaatttca gggtcaggga atatttctcc gactcccctt cctgcgccac ataacctaag
    52741 gttttcatgg tctgtaaaaa gcgataaacg gtgctttttg acatcatgac gcgctgcgac
    52801 agctcggtta tccctatttc gcgctcttca cccagcgcct gcaaaatgcc aaaaactttt
    52861 agcacggaag atacagaatc aggctgttta tccagatctg cgttagccat ttatcacctc
    52921 attgcgagtg ttttataaaa atcagaactg ttttttatta taatttcgca ccagggtggt
    52981 cgcaatccat cttttgccgg ttagttacaa ttctgcgaca tccaccgtga atatcagtgc
    53041 tagaatcata cccctgttga ttattcacca aagatataaa attcctatgc caaaagttca
    53101 ggccgacggc ctgccattgc cccagcgata cggtgcgata ttaaccattg tgattggtat
    53161 ttcgatggct gtccttgacg gcgcaatcgc caacgtcgcc ctgccaacaa tcgccacgga
    53221 ccttcatgcc acgccagcca gttccatctg ggtagtgaac gcctatcaaa tcgccattgt
    53281 catctccctg ctctcgtttt cgtttctggg cgatatgttt ggctatcgac gtatttataa
    53341 atgcggtctg gtcgtttttc tgttgtcttc actgttctgc gccctttctg attcgctgca
    53401 aatgctcacc cttgcgcgtg tcatacaagg tttcggcggt gcagcgttga tgagcgttaa
    53461 taccgcactt atccgcctga tctatccaca acgttttctg gggagaggga tgggcataaa
    53521 ctcgtttatt gttgccgtct cttctgctgc cgggccgaca attgctgcag caatcctctc
    53581 catcgcatcc tggaaatggt tatttttaat caacgtaccg ttaggtatta tcgccctgct
    53641 tctggcgatg cgttttctgc cacccaatgg ttctcgcgcc agtaaacccc gtttcgacct
    53701 gcccagcgcc gtgatgaacg cgttaacctt cggcctgctt atcactgcgt tgagtggttt
    53761 cgctcagggg caatcgctga cgttaattgc tgcggaactg gtggtaatgg ttgttgttgg
    53821 tattttcttt attcgccgcc agctttctct tcccgtaccg ctgctaccgg tggatttact
    53881 gcgtatcccg ctgttttcac tttctatttg cacatctgtt tgctctttct gcgcacaaat
    53941 gctggcaatg gtttccctgc ccttttacct gcaaaccgtg ctcgggcgta gtgaagtcga
    54001 aacaggttta cttctgacac cgtggccgtt agcaacgatg gtgatggctc cgttggcagg
    54061 ctatttgatt gaacgcgtac atgcaggatt gctgggtgct ctagggttgt tcatcatggc
    54121 tgcggggctt ttttccctgg ttctgctgcc cgcgtcacct gcggatatca atattatctg
    54181 gccgatgatc ttatgtggcg ctggatttgg cttgttccag tcacccaata accacaccat
    54241 tattacctcc gctcctcgcg aacgtagcgg tggagccagt ggcatgttag gaacggctcg
    54301 tctactgggt cagagtagcg gcgcggcact ggtggcgctg atgctaaatc agttcggtga
    54361 taatggtacg cacgtttcgc tgatggctgc ggctattctg gcagtgattg ctgcctgtgt
    54421 gagtggttta cgtatcactc agccacgatc cagggcataa aaaaagcgcg tcgatcagga
    54481 cgcgcttttt agtatttact tcatattact tcaggtattc acccgtacgc agagcttcaa
    54541 ttcgtttatc cagcggcggg tgagtcatga acaactcact gagcgatttc gacttaccgt
    54601 taatgcagaa agccatcatg ctggttgctt cttgcggttc atagctggtt ttcaggcgct
    54661 gcagcgcggc aatcattttc tcgcgaccaa ccagttttgc cgaaccggca tcagcatgga
    54721 attcacgatg acgcgagaac cacatggtga taatgctcgc cagaatacca aacaccagtt
    54781 ccagaaccgt tgcaaccgca aagtagatca gcgggttgcc gttgctctct tcaccttcat
    54841 cacgatttcc gcccataaaa cccgcggcaa gctgcgccag aatacgggaa ataaagataa
    54901 cgaaggtgtt caccacgccc tgaatcagcg tcatggtaac catatcacca ttggcgatgt
    54961 ggctgatttc gtgagcaatt accgcctcgg cttcatccgg gctcatgttc tgcagcaaac
    55021 cggtgctgac agcaaccaga gaggcatcac ggcgcgcacc ggttgcaaaa gcgttgatgt
    55081 ccggcgcatg gtagatagcc acttgcggca tagcgatccc cgcctgacga gcctgggttg
    55141 ctacagtatt gaccagccaa cgttcccttt cgttacgcgg ttgctcgatc acttccccgc
    55201 caacagatcg taatgccatc catttggaca tcagaagcga aacgaaggaa ccaccaaaac
    55261 cgaacagcaa ggccatgatc atcagcccct gaacgctgct cgactgtatc cctgtcaggc
    55321 tcagtaccag cccgaaaacg accattacgg ccaggttcgt tagcaggaag agcgcgattc
    55381 gcatcataat tttcttttta cctcagttta acaaaacgca atatgcgata cccacatcgt
    55441 atgggttacg cgactatttt caagtctgga tagtgcgtaa gtcaccagaa agacacaact
    55501 ttacattttg tagcatctga tttacggcat cttgccgctg ttaaaaaatc aggcacaatt
    55561 tcttgtgcct gattgatatt acttgacggg agcgggttgt tccgcgggtc tggctttttc
    55621 aagcttcgcc agatcgagtg cgatattcac cgtctcatcc agataaggat ccggctcctg
    55681 gtaatctttc ggtagatcat ccagcttctt caactccggt ttaccttcgc gtttaaagcg
    55741 ttcgttcaaa cgcgccagac gcgtcgcatc atcttcatta ttctctttct cacgcacagc
    55801 gtagttcaga gaaacgatat tgcgcttgtc cttcatagcg ttgaagcgcg cgatatcctt
    55861 catgatgttc tggaactcag gatctttcgc gatacgcgca ttatgttcct tcagcagctc
    55921 cggttcaaag gcagttaaat ctcctgattt cacataagtc gcggcatcga tgctatccca
    55981 cggcagcgcg ttatcttcga atttctcacc cgtttccgtt tcttcattac ccgtcggcat
    56041 gatgatgtct ggcgttacgc ctttacgttg cgtactgccg ccgttaacgc gatagaattt
    56101 ctggatcgtg tactgcacag aacccagcgc tggccattca ggacgtaaca tctgatcgta
    56161 aatacggttc aatgaacggt attgctgaac ggtgccttta ccaaacgtcg gttcacccac
    56221 aaccagcgca cgaccgtaat cctgcattgc cgcggcaaag atttctgaag ccgaagcact
    56281 gaaacggtca accagtacca ccagcgggcc tttatagaac acctgcccgt cggtatcgct
    56341 gtcttcacga accttgccgt tgttatcgcg gacctgaaca atgggacccg caggaataaa
    56401 cagacctgag agcgataccg cttcggtcaa cgccccaccg ccattgctac gcaggtcgat
    56461 gatcacactg ctgacattct gtttttccag tttctgcagt tgaactttga catcgtctgt
    56521 caaacccaca tagaagcccg gaatatccag cacgccgact ttctctttac cgacggtctt
    56581 caccgacatt ttaaccgcgc ggtcttcgag acgaatacgt tcacgggtca atgttacagt
    56641 gcgggtcttg gtccctttac cagcaggtaa aatttccaga cgaactttac tgcccttcgg
    56701 ccctttaatt aaggcaacca catcatcaag acgccagcca atcacgtcaa ccatcggctt
    56761 gcctgtttga ccaacaccga caattttgtc accaacgctg atagctttac tcttcgctgc
    56821 cggaccacct gccaccatcg aattgataac ggtgtagtca tcatccattt gcagcactgc
    56881 gccaatacct tccagcgaca aactcatttc agtgttgaac tgttcggtat tacgcgggga
    56941 aagatagttg gtatgcgggt cgatttcacg cgcaaacgcc gtcattgcca gcgagaaaac
    57001 atcttcgctg ttggtttgcg ccagacgacg aatggcaaat ttgtagcggc gtgtcagggt
    57061 ttcacgaatt tctttatccg tttttcctgc cagcttcagg cttaactcgt cgaatttgac
    57121 tttactgtcc cacagcgcgt tcaactcagc ctcgtttttc ggccagggcg ctttgctgcg
    57181 gtcaaggtta taagtgtcgt tgccggtgaa atccatcggc ttttccagta ccgacaaagc
    57241 gtactggtaa cgctcaaaac ggcgcttttg cgccagattg tagagatcgt agaaaacgtc
    57301 gagtttgcct gaacgcagtt catcgcctaa ctcggttttc tttttcgcga actgttcaac
    57361 atcgcttgcc agcagcacgt tgtggctgta atcgagcaga ttcaggtagc ggtcaaagat
    57421 tttggccgaa aatgcctgat cgaggtcgaa ctggcgataa tgagaacggg tgaagcgcga
    57481 cgttacgcgc tcacttaccg tcgcatgctg cgtctcttcc tttaataccg gaatttgatc
    57541 agcacgcgtg atatcttcta cagcgaaggt ctggcctgct attgcaagca ggccagctaa
    57601 cgcggtaagc ctaaaaaaca tgttcatgcc tggcccggcc tccgtttcag aacaccaggt
    57661 gttctgcgcg cacaatcaaa gacatacccg aattcagctg gacgcggacg ccgtctttgg
    57721 tgatttctaa tacggtggca tccatcgcgt tttgacccgc tttcaccttc agggcttgtc
    57781 cgacagtcag agctgaaatg tcagaaaccg gggtgtgctg ttcttcgcga ggtgctttta
    57841 ctgtttttgg cgctttctct accggctttt gcgcacgagg tttacgttca gcgccttctt
    57901 tgcggcgtgg cgtggtcgga cgtggcttgc gttcgcggcg cggtgcgtct tctttctcac
    57961 cagcagctgc ggcagcttcg cgttttttcg cttgctgttc agcacgctgt gcctgaacac
    58021 gcgctttcgc ttcttcaagc tgcttgcgag catgctctac atgttgctcg tccagctcac
    58081 cgcatgggtt gccgtcaaga tcgacacgcg ttgcgccggg tttaacaccg taaagataac
    58141 gccagctcga agtgtagaga cgtaaagcgg atcgtaattg cgttttgctc aggttcattt
    58201 ccccggcaac acgatcgacc aaatcctgaa aaataccgat tttcagcgga cgcgcttcac
    58261 cttccgcact gaaacagtgg ggaaaacgtt cggccagaaa cgcgattact tctttactgc
    58321 tattcaactt aggttgattt tccatgaaat ttcctgatta caacggacgt agccaacaag
    58381 cgcaggcatg aacaggcgtc attataatga cgctaccagt aaatgctacg ttatccgttg
    58441 attatcctgc gacgctcgca aagaattttt tgtaatccgt cgttgcaagc actttttcaa
    58501 gctgtgccac aagctgacgt aagccttgct cgtcctcgtc tgtaaagcga ccgaagacgg
    58561 tactatcgat gtcaagaaca ccaataatct gatttttcac caccagcggc agaacaattt
    58621 cagaattact cgccgcatca caggcaatat gcccgtcaaa cgcgtgaaca tcctcgatac
    58681 gctgcacttg attgcgggca accgcagtgc cgcacacccc gcgcccgacg ggtatccgga
    58741 cacaggcaat tttgccctga aatggtccga gtaccagtgt atcgtcctca agcaaataaa
    58801 aacctgccca gtttacgtca gtgagacgct cgtataacaa cgcactggtg ttcgcaagcg
    58861 ttgccagaaa actggtttct cccgccatca gcgcgttaaa gtcgcgattt aaatccgcgt
    58921 aaaattctgt tttgttcatt atataatcac ttgtttgtct tacctggaac tgccagccta
    58981 ttaaaataag cattaaatgc gttaatgctc aagatcattc ccatcatggg ttaagattaa
    59041 tgttaattct tattacattt ggcacgtcat ggctcttaac acaccacaaa ttacgccgac
    59101 aaaaaagata acagtgagga caatcggcga ggaactgccg cgtggtgatt accaacgttg
    59161 cccgcaatgt gacatgctgt ttagcctgcc cgagataaat tctcatcaga gtgcctattg
    59221 tccgcgctgt caggcaaaaa ttcgcgacgg gcgcgactgg tcgctaacgc gcctggcggc
    59281 aatggctttc accatgctgt tgctgatgcc gtttgcctgg ggcgaaccgc tgttgcatat
    59341 ctggctgtta ggtattcgta tcgacgccaa cgtcatgcaa ggcatctggc aaatgaccaa
    59401 acagggcgat acgataacgg gggcgatggt ctttttctgc gttattggtg cccccctcat
    59461 tctggtgtcc tccatagcat atttatggtt tggtaaccga ctgggaatga atttacgtcc
    59521 ggtactgctg atgcttgagc gacttaaaga gtgggtaatg ctggatatct acctggtcgg
    59581 cattggcgtt gcttctataa aggtacagga ttatgcccat atccaggcgg gtgtcggctt
    59641 gttctctttt gtggcgttgg tgattttaac gacggtgacg ttgtcacatc ttaatgtcga
    59701 ggagctgtgg gagcgatttt atccgcagcg ccccgctacg cgtagggacg agaaacttcg
    59761 tgtctgtctt gggtgccatt ttaccggcta tcctgatcag cgtggtcgct gcccgcgttg
    59821 ccatatcccg ctacgcctgc gtcgccgtca tagtttgcaa aaatgctggg cggcgctgtt
    59881 agcgtcaatc gttttgttgt tacctgccaa cctgttgcct atttctatca tttatctgaa
    59941 cggaggacgg caggaagata caattctttc cggaattatg tcgctggcaa gtagcaacat
    60001 tgcggttgcg ggaatcgtgt ttatcgccag tattctggta ccgtttacta aagtgatcgt
    60061 catgttcact ttactgttga gcattcattt taaatgccag caaggtttac gcacacgcat
    60121 tctgttactg cggatggtga cctggattgg tcgctggtcg atgctcgacc tgtttgtcat
    60181 atctttaacc atgtcgctga ttaatcgcga tcagatcctc gcttttacta tgggaccggc
    60241 tgcgttttat ttcggcgcag cggtaatttt gactattctt gctgtggaat ggctggacag
    60301 ccgcttactt tgggatgcac atgagtcagg aaacgcccgc ttcgacgact gaagcgcaga
    60361 ttaaaaataa acgccgtatc tcacctttct ggctgctgcc gttcatcgcg ctaatgattg
    60421 ccggttggct gatttgggac agttatcagg accggggtaa taccgtcacc atcgacttta
    60481 tgtcggcgga tggtattgtc ccgggccgta cgcctgttcg ttatcagggc gttgaagtcg
    60541 gaacagtgca ggatatcagc ctcagcgacg atcttcgtaa gattgaagtc aaggtcagca
    60601 tcaagtccga tatgaaagat gcgctgcgcg aagagactca gttctggctg gtgacgccaa
    60661 aagcatcgtt ggcaggtgtc tccgggctgg acgccctcgt cggtggtaac tatatcggca
    60721 tgatgccagg taaaggtaaa gagcaggatc actttgtcgc actcgatacc caaccgaaat
    60781 atcgactgga caatggcgat ctgatgatcc acctgcaagc ccccgatctc ggttcgctga
    60841 acagcggttc attggtctat ttccgcaaga tccctgtggg aaaagtctac gactatgcca
    60901 tcaatcccaa caagcaaggc gtggtgattg atgtcctgat cgagcggcgt tttaccgacc
    60961 tggtgaaaaa aggtagccgt ttctggaacg tttccggcgt tgatgccaac gtcagtatca
    61021 gtggcgcgaa ggtgaaactg gaaagtctgg cggcactggt taacggtgcg attgccttcg
    61081 attcacctga agagtcgaaa cctgccgagg cggaagatac ctttggtctg tatgaagatc
    61141 tggcccacag ccagcgtggc gtaataataa aactggaact gccgagtggg gccggattaa
    61201 ccgccgactc gacgccgtta atgtatcagg ggctggaagt cggacagctg actaaactgg
    61261 atttaaatcc tggtggtaaa gtcactgggg aaatgaccgt tgatcccagc gtcgttacgc
    61321 tgttacggga aaatacccgc atcgaattac gcaacccgaa attatccctt agcgatgcca
    61381 atctcagcgc cctgctgact ggcaaaacct tcgagttggt acccggcgat ggcgagccac
    61441 gcaaagagtt cgttgttgtg ccaggcgaaa aagcactgct gcatgaacct gatgttctga
    61501 cgctgaccct gaccgcgccg gaaagttacg gtattgatgc gggtcagccg ctcattcttc
    61561 acggcgtgca ggtaggccag gttatcgatc gtaaactcac cagcaaaggc gtcaccttta
    61621 ccgtcgccat cgagcctcag catcgagaac tggtaaaagg cgatagcaaa tttgtcgtca
    61681 acagccgtgt cgacgtgaag gtggggctgg atggcgctga gtttctcggt gccagcgcct
    61741 cagaatggat taacggcggg atacgtattc tgccgggcga taaaggcgag atgaaagcca
    61801 gctatccact gtatgccaat ctggaaaaag cgctggagaa cagccttagc gatttaccca
    61861 ccacaaccgt gagtttgagt gcagagacgc tgccggatgt gcaggcagga tcggtagtgc
    61921 tctaccgtaa atttgaagtt ggtgaagtta ttaccgtccg tccgcgagct aacgcgtttg
    61981 atatcgatct gcatattaag ccggagtatc gcaaccttct gaccagcaat agcgtgttct
    62041 gggcagaagg cggggcgaaa gttcagctga atggtagtgg cctgaccgta caggcatctc
    62101 cgctctccag agcattaaag ggagccatta gcttcgataa cctcagcggt gccagcgcca
    62161 gtcagcgtaa aggcgacaaa cgaattctgt atgcttccga aacagcggcc cgtgcggttg
    62221 gtgggcagat tacgcttcac gctttcgatg ccggaaaact ggcggtcggg atgccaattc
    62281 gctatctcgg tattgatatc gggcaaatcc agacgctgga tctgattacc gcgcgcaatg
    62341 aagtacaggc aaaggcggtg ctctatccgg aatatgtcca gacctttgct cgcggtggta
    62401 cgcgcttctc agtggtcaca ccgcaaattt cggcagctgg cgttgagcat cttgatacta
    62461 tcctccagcc gtatatcaac gtcgaaccag gccggggcaa tcctcgccgc gactttgaat
    62521 tacaagaggc caccattact gattcgcgtt acctggatgg cttaagcatt attgttgaag
    62581 cgccggaagc cggttcgtta ggcatcggta cgcctgtgct gttccgtggt ctggaagtcg
    62641 gtacggttac aggaatgacg ctggggacat tgtcagatcg cgtgatgatt gcgatgcgca
    62701 tcagtaaacg ctatcaacac ctggtgcgta acaattccgt cttctggttg gcatcgggtt
    62761 acagtctgga ctttggtctg acgggcggcg tagtgaaaac cggcaccttt aaccagttta
    62821 tccgtggcgg catcgccttc gccacgcctc cgggtacgcc actggcaccg aaagcccagg
    62881 aaggcaaaca cttcctgttg caggaaagtg aaccgaaaga gtggcgtgaa tggggaactg
    62941 cgcttcccaa ataatgccca ctgctccggc gtgcctgcgc cggagcgttt atgctaaact
    63001 gcgcgcctgt ttttttgcca gtggtacatg ctcgtggccc aacacaccgt ttatttcccg
    63061 gacgcctttc tgacgcaaat gcgcgaagcg atgccttcga cgctctcttt tgatgatttt
    63121 cttgccgcct gtcagcgccc gttgcgccgc agcattcgcg ttaatacgct gaaaatctcc
    63181 gttgctgatt tcctgcaatt aaccgctcct tatggctgga cgcttacgcc aattccgtgg
    63241 tgtgaagaag gtttctggat tgaacgcgac aatgaagatg cattgccatt gggtagtacc
    63301 gccgagcatt taagcggcct gttttatatt caggaagcca gttcaatgtt gcccgtcgcc
    63361 gccttgtttg ctgacggtaa tgcaccacag cgggtgatgg atgtcgctgc tgcgccaggc
    63421 tccaaaacga cgcaaattgc cgcgcggatg aataacgaag gggcaatcct tgccaatgag
    63481 ttttccgcca gtcgggtaaa agtgttacat gccaatatca gccgctgtgg catcagtaat
    63541 gttgcgctca cacattttga tggccgcgtg tttggtgtgg cagtgccaga aatgttcgat
    63601 gccattttgc tggacgctcc ctgctctggc gaaggcgtgg tgcgtaaaga tcccgatgcg
    63661 ctaaaaaact ggtcaccaga aagcaatcag gaaatcgcag ctacacaacg ggagcttatc
    63721 gacagcgcct ttcatgcatt acgtcctggt ggtacgctgg tttactcgac ctgtacctta
    63781 aacagggaag aaaacgaagc cgtttgcatg tggctgaaag agacttaccc tgacgcagta
    63841 gagtttttac cacttggcga gctcttccct ggtgcaaaca aagcgctgac cgaagaaggc
    63901 tttttgcatg ttttcccaca aatttacgac tgcgaaggct tcttcgttgc tcgtctgcgt
    63961 aaaactcagg ccattcccgc cttacccacc ccaacataca aagtgggcaa ttttccgttc
    64021 agcccggtga aagatcgcga agctggacaa attcggcagg cggctgcagg tgttggctta
    64081 aactgggatg aaaacctgcg cctctggcag cgtgacaaag aactgtggtt gttcccggtg
    64141 ggcattgaag ccctgatcgg taaagtccga ttttctcggt tggggattaa acttgccgag
    64201 acgcacaaca aaggttatcg ctggcagcat gaagcggtta ttgctcttgc ctcacccgat
    64261 aatatgaatg cattcgagct gacaccgcag gaagcggagg agtggtatcg cgggcgcgat
    64321 gtttacccgc aagccgcgcc agtggcggat gatgtattgg ttactttcca gcatcaaccg
    64381 attggtttag ccaaacggat tggttcgcga ttgaaaaaca gctatccgcg tgaactggtg
    64441 cgcgatggga aactttttac cggtaacgcc tgacagcgca caaaaaaagc gcactttttg
    64501 actggcacat tcggcggcct caactaggct gaaaaatggt gcgatcggac tggtcgtacc
    64561 acaatcggca gctaaatgga gagcacgaag atgaaaacca gtgtgcgcat aggcgctttt
    64621 gaaatcgacg acggcgaatt acacggtgaa tcgccgggtg atcgaacgtt aaccattcct
    64681 tgtaaatctg accccgattt atgtatgcaa ctggatgcct gggatgctga aaccagtatc
    64741 cctgccctgc taaatggcga acactctgtc ctttaccgta cccgttacga tcaacaatct
    64801 gatgcctgga ttatgcgtct tgcctgatcc aaaaagaacc cgtcggcatg gcgggttatt
    64861 tgtcctggtt atttccccgt tgtaaaatct ctcctaaact taacggtacg gcaccacact
    64921 tcggggatga aatgttcgcg ctggtacttt ttgtttgcta cctggatggc ggttgtgaag
    64981 atattgttgt ggatgtctac aacacggaac agcagtgtct tcattctatg agcgatcaac
    65041 ggatccgcca tggcggttgt tttccgattg aggattttat agatggtttc tggcgacctg
    65101 cacaggagta cggtgatttt taattattgc aattgcacaa gagtcagttc gcccccaaag
    65161 acagcaccgg tatcaatata atgcaggttg ccaatatcca cgcgatgtcg caacggtgta
    65221 tgaccaaacc agaaatgatc agcacctgta attccctgca ctttttggcg ttcacttaat
    65281 cgcgagcggc tccacaagac ctgatgcaaa tcgacgtcct tttgccattc ataaacatca
    65341 tctggataat cggcatgagc aataacatgc ttgccggtgc gactgtgtac ttcaagaata
    65401 aagggcaaat gctgacattt ccccagcgcc gttttcgctt gtttctgttg attatctgcc
    65461 agcgcaataa accagttgcc gccattcatc aaccataaag acatctgctg ggatgccagc
    65521 gcatccatcg ccatctgttc atgattgcct cttaccgcac gaacccaatg ttgttccagt
    65581 aactgcagac aacgtaaact ttgcggccca cgatcgataa cgtctcccac tgagataagt
    65641 aaatctcgcc acggatcaaa acgacaatgc cataatttgc ggcgcaactg ctcaagacaa
    65701 ccgtgtatat cgccagaaag ccagatatgt cgccattgat gacccgcaat tctctgataa
    65761 acgggcgcag gctgtttcat caatattttc ctcccgcgct aaagatcaca taatcttaac
    65821 aagaatgtta aaaaatgctg gactcagaca gtagagtgtg tgttatggtt gactataaag
    65881 tcagcgaagg aaatgcttct ggcttttaac agataaaaag agaccgaaca cgattcctgt
    65941 attcggtcca gggaaatggc tcttgggaga gagccgtgcg ctaaaagttg gcattaatgc
    66001 aggcttagtt gccttgccct ttaagaatag atgacgacgc caggttttcc agtttgcgtg
    66061 caaaatggtc aataaaaagc gcggtggtca tcagcttaaa tgttaaaaac cgcccgttct
    66121 ggtgaaagaa ctgaggcggt ttttttattg gaaatcaaaa ggctatttta ggtaattaac
    66181 agagtttttc agctcgttct ataaacggtg ccagactcat tttttcgccg ggattgttgg
    66241 gatcatcaat ctgaatcacc gaaatgggtt gggctttagt cttcccactg gcaacttcct
    66301 tctgtgcgat atcgtttaaa ggatactgca cgagggtact tgggttaatg acatacaaag
    66361 cattacccgg tctgcaagtc agcatcacct cttcgcgatt aaacgcccat ttgtctttac
    66421 ccacttcaaa acggctgacg gtaatcacct gcggtgcagc cagcgccgct gcagaactgg
    66481 tgagtaacag aaacgccaga atactttttt tcatcatttt cgtaatccat caaaaaggtt
    66541 cgagagtcgc aagcaggctg atgatcacca gcactaccgc acctatcgcc cattcgagtt
    66601 tagtcatcca cacaaaccag ggagccgcac gatcttcatc ctgcctcatg cgcggtacaa
    66661 gaacataacg attagccagc gcaatgacca ccataatcat caccaggatg gctttgagca
    66721 ataagagttg gccccagtaa gtcgtaatcg tggggggaaa tccggtaatc aacaatgcat
    66781 taagcacgcc gcttgccagt acgccgatca ccgcaaaatg cccgcaccag gaaaaacgca
    66841 tcagcgcctg aatagcctgg tgtcgccaac ggcctttgat gagttgcata caccagagca
    66901 ccggcagtaa tcccccaaac cacgccgccg cacaaatcag gtgaatcgcg tgattagtct
    66961 gatggatttt cgccgttacc ccttcattca gcgtcgcatg ccccactccc gccagcagaa
    67021 taaattgcgc ggtagtgagc ataaacagca aacgtggcat attccgcggt tgcataaggg
    67081 cgacgatcaa cgttacgagg gcgagaacaa tttgccataa ccagacgcca ccaaactgcg
    67141 tttgcaaaac agcctgccaa atgtttggcg aaaatacatc tttccatccc gttcccatca
    67201 acccgccctg aacggcgagc attgcagtgg cactgatcaa actccagacg gcagcgtgct
    67261 gttgtaaacg cagaaaacgc ttcgccagca aacgacgaat cgttaagggg gccagccagg
    67321 caccgtacat tgcgaagcca aaaaccagca tcagcgaggt gaaatggata aatcgcagcg
    67381 cgatccaggt aaacgccagc atgatttatt tcacgctaaa ggtgtagtgt cctttcgttt
    67441 tatgcccgtc caccgaaaca acatgccagt caacggtgta ggttccgggt ttcagcgaat
    67501 cggccagtgg aacaatcagt tgcttttggt cctgctcatt tcgcttcgcc ggtaatgttt
    67561 taatattttc gttttttggc cccgtgattt ttgcaccact gaatcctgtt tcaacgcctt
    67621 ccgagaagtt taaagtgatt gcctgcggag atgctgtcac ttgcgcgttt gccgcaggat
    67681 actgatgcat taaatgcgca tgtgcccaga cggaaggtgt caccaacgaa gtggtcagga
    67741 ttgccagcgc gtagcgaagg gagcgtgcag ttgaagccat attatctatt cctttttgta
    67801 ataacttttt tacagagcat aaccttgtct aatgtctgag tcgaggatca tcaattccgg
    67861 cttgccatcc aggctcactc ttagtaactt ttgcccgcga atgatgagga gattaagaat
    67921 gctgaagaat ctggctaaac tggatcaaac agaaatggat aaagtgaatg tcgatttggc
    67981 ggcggccggg gtggcattta aagaacgcta caatatgccg gtgatcgctg aagcggttga
    68041 acgtgaacag cctgaacatt tgcgcagctg gtttcgcgag cggcttattg cccaccgttt
    68101 ggcttcggtc aatctgtcac gtttacctta cgagcccaaa cttaaataaa acttatacag
    68161 agttacactt tcttacataa cgcctgctaa attatgagta ttttctaaac cgcacccata
    68221 atttgcagtc attttgaaaa ggaagtcatt atgtcgttct ggaaagttgc agcagcgcaa
    68281 tatgagcctc gtaaaacctc actcaccgaa caggtagctc atcatctgga gtttgtgagg
    68341 gccgccgcca ggcaacaatg tcagctgtta gtttttccat cgctttcttt actggggtgt
    68401 gattattcgc gacgtgccct tcctgcccca cccgatctct cactgttaga cccgctttgt
    68461 tatgccgcaa cgacctggcg aatgaccatc attgccggcc ttcccgttga atataacgat
    68521 cgctttattc gtggaattgc ggtgttcgca ccgtggcgaa aaacgccggg gatctaccat
    68581 cagagtcatg gtgcatgtct gggccgtcgt tccagaacca tcacggtggt agatgaacaa
    68641 ccgcaaggta tggatatgga cccgacctgt tcactgttta cgacagggca atgtctgggg
    68701 gaacccgacc tagtggcatc tacccgccgc ttacaatttt tttcacatca gtactccatt
    68761 gccgtgctga tggcaaatgc ccgtggtaac agtgcactat gggatgaaca tggtcgtctc
    68821 atcgttcgcg ccgatcgtgg ttcattattg ttagtcggtc agcgttcatc acagggttgg
    68881 caaggcgata tcattccatt acgctaggct ttttcggcct ggagcatgcc atgttgcgca
    68941 ttatcgatac agaaacctgc ggtttgcagg gagggatcgt tgagattgcc tctgttgatg
    69001 tcattgacgg aaaaatcgtc aaccccatga gccacctggt gcgccccgat cgtcctatta
    69061 gtccacaagc gatggcgatt catcgcatca ccgaagccat ggtcgccgat aaaccgtgga
    69121 ttgaagatgt gatcccacac tattacggta gtgaatggta tgtcgcgcat aacgccagct
    69181 ttgaccgccg cgtactgcct gagatgcccg gtgagtggat ttgcactatg aaactggccc
    69241 gtcgtttgtg gccagggatc aagtacagca atatggcgtt atataaaaca cgcaagctca
    69301 atgtacagac gccgccgggc ctgcatcatc accgcgcgtt gtatgactgt tatatcaccg
    69361 ccgcgttgct tatcgatatt atgaacacct ccggctggac ggcagaacag atggccgata
    69421 tcaccggacg tccgtcgttg atgacgacct tcacctttgg caaataccgt ggcaaagcgg
    69481 tttccgacgt tgccgaacgc gatccgggct atctgcgctg gttatttaat aacctggaca
    69541 gcatgagccc ggagctgcgt ttaacactga aacattatct ggaaaatact taatccgcag
    69601 gcgtagcggg taatgttccc tgcgccagcg cgaccagaaa agcatattcc atcgctacgc
    69661 cttcgtacga tttaaagcga ccagatttac cgccatggcc tgagtccatg tcggtacaga
    69721 gcaataaaag atggtcatcg gttttcagct cgcgcaattt agcgacccat tttgccggtt
    69781 cccaatattg cacctgagaa tcgtgcaaac cggtcgttac cagtaaatgc ggataagcct
    69841 gtgcggtgac gttgtcatac gggctgtagc ttttcatgta ctcgtaatat tgcggatcct
    69901 gcgggttacc ccactcttca aactcaccag tggtaagagg aattgattca tcaagcatcg
    69961 ttgttacaac atcaacaaac ggtacctggg cgataacgcc gtggaataat tccgggcgtt
    70021 gattaattgc aacgcccatc aacatgcccc ccgcactccc gcccatcgca taacaaagcg
    70081 aaggagagcc atagcccagt tttaacaatg catcgcaggc atcaagataa tcattaaacg
    70141 tatttttctt cttcagaaat tttccgtctt cgtaccattg ttgccccagc tcaccaccgc
    70201 cgcgaacatg gacaatggcg tagacaaagc cacgatctaa caaactcaag cggctaaaac
    70261 tgaaatcggc atcaatactt gcgccgtaag aaccatagcc atacaccagc aacgggttgt
    70321 gtcctttgcg aaaatgtttg cgatggtaga ccaacgaaac cggaacttcg acgccatcac
    70381 gggcgactat ccacaggtgt tcactgcggt aattcgccgc atcaaaaccg ggcacttctg
    70441 tttgttttaa tacacgacgc tcaccggtat ccatatccag ttcaaacaaa gtgtctggtg
    70501 tggtcatgga agaataacca taacgcaatc gcgcggtttc aggttctgga ttgtaggcaa
    70561 tccaggtcac ataggctgga tcatcaaagg caataccaat gacttcccgg gtcttgcggt
    70621 taatttggcg caaactggtt aacccgcgct gacgctcttc aaccaccagc cagtcggtaa
    70681 acagcgtaaa cccttccagc atgatgtttt cgcgtggcgg aattaactct tcccactgtt
    70741 gctcatcacg catacgggta cggtataagc caaagttttt gccgtggcgg ttggaacgta
    70801 gataaaaacg atgctggtag tgatcaaggc tgtattcgtg atctttgcgg cgcggcagaa
    70861 aaacaaacgg ctcggcatcg gccatttccg cgtccagcag gcgaacttca ctggtggtgg
    70921 cgctggccaa atgaatgact acatagtgct tcgaggtcgt tttatgcagg ctgacgtaat
    70981 aggtatcgtc tttttcttcg tagatcagtt tatcttgcga tgctggagta ccgatagcgt
    71041 gacgccagac ctgataaggc agcagcgtca ctggatgctt gcgaacatag tagaaagtcc
    71101 aggagtcatt cgcccagaca aagctgggtt caacgttatc cagcagttcc gggtaccagt
    71161 tacctgtttc cagattacga aaacgaatgc cgtactggcg tcgggaaaga aaatcttctg
    71221 ccagcgccat gatggtgtta tcgggcgtaa tcgccattcc gcccatcgaa taaaactcac
    71281 tatgagctgc gcgcttattg gcatcgagca atgtttccca ctcatcccac tcttcactga
    71341 atgccgattg acgctggtag atagcatatt cacagcctgg ttcataaata tgccgatagc
    71401 ggtagccatt tttgatgtag ggcgcagaaa cttctcgttg cggaatgcgg tcgatgattt
    71461 cctttaagat gcgatcctgc aaggcttgtt gtgaggccat cacccgatga ccgtaactat
    71521 tttcttgttg caggtagtcc agaacttccg gctgagaacg cgtatcgtcc cgcagccagt
    71581 agtaattatc gattcgcgta tcgccatgaa gcgtcatggc gtggggaatg cgggcggctt
    71641 ttggtagcat gttattgttc tttctggttg aaacatctta taagggtggc aaaactcacc
    71701 ggggatgcaa gcgaaacagg ggagccattg cttagatgat gacaggtaat ggcgcggata
    71761 tcgaatgtta tgcagacaga gaaaatcagc ctgttcaaat ggctgtgcga ttctggatag
    71821 cccgaaatag tcaacttcag gctatccaga gagtggaatt attccgccaa agtgcgtttt
    71881 tgctgttcga gatcgcgttc aatgccgtca cgaacatcct gggggatttt cagcgcgtca
    71941 cccagtgcat tcaggtaact gcgttccata aaatggtcaa tatcaatagc cgcgcaactc
    72001 aggaaataga tttccagcgc ctcttcttca ttgcggaccc cggtagccag gcgttgtgga
    72061 tccagcggtt gttcgattgc ctgctcaatg agtacacgcc cctgctcttc cacgccggct
    72121 tcacgcaatt gctggtcgat tgccgcacgt tctttggcat caatatgacc atcactttta
    72181 gcggcaaaga ccagcgcaag gatcaaacgt tctgtacgct catccagcgg cgtactttgc
    72241 gcgccaaact gcggttcgtc ctgatgcgcc gcgcgaattt tatctttgta tttattccac
    72301 agcaccgtac ccgctaccgc tccgccgcca accagtaacg cgttggtgcc atatttcgta
    72361 agaagtttac gtgctgattt atttgcgacc agcagcccag ccagtccgcc taatgcgcct
    72421 ggcactaaca gtttgcccaa tccctgatcc gcagacgagg aggtagaaga actgctttgc
    72481 ccaagcaggg attgcagttg atttaaccag ttagccatat ttgctcctca ataaccattt
    72541 ttagtgcctg tagcatagcg gatgaggatg tcagaaaatg tatatcgaga caaaagatgc
    72601 gcaaataagc ggggcggtag gccgttattc aaagaaattc gcgccaggta aggtatttac
    72661 tgtatatcag gtgataaggg tattttaggt gaaagtagtt tgtcgttttg agtggcattg
    72721 tttgatgctg attgcccgat gcgacgctgt tgcgtcttct caggcctacg attgtggtgc
    72781 gttctggggc ggacaaggca ctcgcgccgc atccgccaac ggtcaatcga attatcagaa
    72841 gccattaacg ccgctgatat tccgccattc ccgctttgca gtccacgctg acctggtaat
    72901 gaatatcggc acttttacca cgcacggtta gcggtactga ccatttatca tctttaccct
    72961 gaatgtcctg caaactgacc caagccacgg gatcggcctg accgacaatt ttttgatcat
    73021 ctgcccagcg cgccacgcga ttttgttgat aatcacgttt tacgctcgcg gcaattccgg
    73081 cagcatccag accttcacac tttgggaaag tgaccgactt gctggtttca ttattggcag
    73141 cgaaaactga tgcgcaggca gaaaccaaca acagccctaa aaacgcccct ctttttttca
    73201 tgtttttctc catagcacaa tgattcagga gaaagcatgg tacaaattgt caggagcgca
    73261 agttgcttca ggccgcgtgt gaggcatctt cctcttcgga ttcagcaccg acgacctgtg
    73321 gggacggtaa tttgccggtt ttcagaatgg tgctcagcac atctttttgt tctgccagcc
    73381 atagcgaaag ggcttcacgt tgctcgtctt ccatttgcac tggagaattt gtcagccagg
    73441 tgtcaagcag atccgccgta tcaagcattt tatcataagc atcggcttcc tttttgctgg
    73501 taaacgacat tttctcttcg ccctcacgaa tgactacgta tttaacttca accgccattt
    73561 gcagcctctc ataataactg tgattttata cagtatattt cttttcggtt gagaaatcaa
    73621 catcagcaat aaagacacgc gcaaacgttt tcgtttatac tgcgcgcgga attaatcagg
    73681 ggatattcgt tatgacgtta ttaggcactg cgctgcgtcc ggcagcaact cgcgtgatgt
    73741 tattaggctc cggtgaactg ggtaaagaag tggcaatcga gtgtcagcgt ctcggcgtag
    73801 aggtgattgc cgtcgatcgc tatgccgacg caccagccat gcatgtcgcg catcgctccc
    73861 atatcattaa tatgcttgat ggtgatgcat tacgccgtgt ggttgaactg gaaaaaccac
    73921 attatatcgt gccggagatc gaagctattg ccaccgatat gctgatccaa cttgaagagg
    73981 aaggactgaa tgttgtcccc tgcgctcgcg caacgaaatt aacgatgaat cgcgagggta
    74041 tccgtcgcct ggcggcagaa gagctgcagc tgcccacttc cacttatcgt tttgccgata
    74101 gcgaaagcct tttccgcgag gcggttgctg acattggcta tccctgcatt gtaaaaccgg
    74161 tgatgagctc ttccggcaag gggcagacgt ttattcgttc tgcagagcaa cttgctcagg
    74221 catggaagta cgctcagcaa ggcggtcgcg ccggagcggg ccgcgtaatt gttgaaggcg
    74281 tcgttaagtt tgacttcgaa attaccttgc taaccgtcag cgcggtggat ggcgtccatt
    74341 tctgtgcacc ggtaggtcat cgccaggaag atggcgacta ccgtgaatcc tggcaaccac
    74401 agcaaatgag cccgcttgcc cttgaacgtg cgcaggagat tgcccgtaaa gtggtgctgg
    74461 cactgggcgg ttatggtctg tttggtgtcg agctgtttgt ctgtggtgat gaggtgattt
    74521 tcagtgaggt ctcccctcgt ccacatgata ccgggatggt gacgttaatt tctcaagatc
    74581 tctcagagtt tgccctgcat gtacgtgcct tcctcggact tccggttggc gggatccgtc
    74641 agtatggtcc tgcagcttct gccgttattc tgccacaact gaccagtcag aatgtcacgt
    74701 ttgataatgt gcagaatgcc gtaggcgcag atttgcagat tcgtttattt ggtaagccgg
    74761 aaattgatgg cagccgtcgt ctgggtgttg cgctggcgac cgctgagagt gttgttgacg
    74821 ccattgagcg cgcgaagcac gccgccggac aggtaaaagt acagggttaa acccggcaaa
    74881 aaaacgctac aaaaatgccc gatcctcgat cgggcatttt gacttttaca gcttagcgcc
    74941 ttctacagct tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt ccagcgcatc
    75001 tgccggaacc agccaggaac caccgatgca cagcacgctt ttcagcgcca ggtagtcacg
    75061 gtagttagcc ggagaaatac cacccgtcgg gcagaaacgg acctgggaga acggacccgc
    75121 gatcgcctgc agggctttca cgccgccgtt agcttcagcc gggaagaatt tgaactcttt
    75181 caaaccgtag tccataccca gcatcagttc ggaaacagtg ctgatccccg gaatcagagg
    75241 aatagtccct tcggtagcag ctttcagcag cggctcggtc agacccgggc taattgcgaa
    75301 ctgtgcaccc gcttcagtga cttctgccag ctgctgtgga ttcagcaccg taccggcacc
    75361 cacaatcgct tcaggcactt ctttggcgat agcacggata gcatcaactg cacactcggt
    75421 acgcagagtc acttccagaa cgcgcacccc accagcaacc aacgcttttg ccatcggcac
    75481 tgcgtgttcc agttttttta ccacgataac cggtacaacc gggccggtgg tcaggattga
    75541 ttctgcactt gttttccagt ttttcatcag agttttctct cgcctgatta caaatttgtc
    75601 gtcttaaaaa gtgatacagg ttgcgccctg ttcggcaccg gacagttttt cacgcaaggc
    75661 gctgaataat tcacgtcctg tccccacgcg tgacgcgctc aggtcaggaa tgtgcggttc
    75721 gcgagcagcc agttccgctt cgtctaccag cagcgtcagt tcgcctgtct gtccattcac
    75781 acgaatgatg tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg cttctggtgt
    75841 tacgtggata gctgacggca ctttacctga agcgccggag agtcgtccat cggtaactaa
    75901 cgcaattttg aaacaccggt ccaataatac accaagtggc ggcatgagtt tatgtaattc
    75961 tggcattccg ttcgcttttg gcccctgatg acggacaaca acgacacaat cgcggtccag
    76021 caaacccgct tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa ccgctggcgc
    76081 ttcaatcacc tggttctcaa ccggcacggc agaggttttc ataaccgcac ggcccaggtt
    76141 accgcttaac acttttgtcc caccatgatg agagaaaggt tgttcgaagg aagcgatcac
    76201 attgctgtcg agtgattttt ccgccccttc ccgccagtcc agttcaccat tattcagcca
    76261 tggttcaagg gtataacgag acagaccaaa gcctgccacc gtattgacat cttcatgcag
    76321 caggcctgct ttgagcagtt cacgcaccag aaccggtacg ccacctgccg cctggaagtg
    76381 gttaatatcg gccggaccgt tcgggtagag acgtgccatc agcggtacaa catcagaaag
    76441 gtcagagaag tcatcccagt taatctgaat accggccgcg cgcgccatcg ccaccaggtg
    76501 catggtgtgg ttagtggaac caccggtcgc cagcagtgca acgataccgt tcaccaccac
    76561 tttctcatcg atcatcttac cgatcggcat ccattcatta ccattaccgg tcatgcgtgt
    76621 aacctgacgc gcagcggcag cggtcaaagc atcgcgcagc ggagaatccg gatgaacaaa
    76681 agaagagcct ggcaactgca tccccataaa ctccaccacc atctggttgg tgttggcagt
    76741 accgtagaaa gtacatgttc ccggcgcatg gtaagacgcg gcctctgact ccagtaaggc
    76801 catgcggtcc actttacctt cggcataaag ctggcgaata cgcacttttt ctttatttgg
    76861 caaaccgctt gccatcggtc cagacggcac aaacaccgca ggcaaatgac caaacgacag
    76921 ggctgccatc gtcagacctg ggacaatctt gtcgcataca ccgaggaaca gagcaccatc
    76981 aaacatgtta tgggacagcc ccaccgccgc agacatcgca atcacttctc ggcttaacag
    77041 cgacaattcc attccatcct gcccctgggt gacaccatca cacatcgccg gaacaccacc
    77101 cgcaacctga ccaaccgcat tcgcttcatg cagggcttta cgaatgattt ctggatagtg
    77161 ttcataaggc tggtgcgcgg agagcatgtc gttataggag gtgatgatgg cgatattgtt
    77221 acgcaacatg cttttcaaag aggctttgtc ttctggctgg caggcagcga aaccgtgtgc
    77281 caggttaccg catgccaact gcgaacgatg aacggtcgac gttttcgctt gttctatccg
    77341 gccgagataa gcagagcgag tctcgcgaga acgttcaatg attcgatttg ttacgcgtaa
    77401 caattgtgga ttcataaagg ctcctgaaat tgagttgtca gagcaggatg attcacaacg
    77461 cgtttcattc agaggattta tgagtgaaac gcctgtaacc ggagctcata gggcaaaaac
    77521 gtttcagtca gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc ctgaaagtgt
    77581 aaaaattgtt ctacaatctg cgcaagatca tgttaccggt aaaataacca taaaggataa
    77641 gcgcagatat tactcaaact cattccagga acgaccatca cgggtaatca tcgccaccga
    77701 ggcaacgggt ccccaggttc cggcctgata cggtttcggc gcatcattgt ccatcgccca
    77761 cgcctcagta atggagtcta cccatttcca ggcttcttcc acttcatcac gacgtacaaa
    77821 cagtgcctga ataccacgca tggtttccag cagcagacgt tcataggcat ccgccagatg
    77881 cgtctgatta aaggtttctg aatagctcag atccagcttg gtgatttgca ggttatgttt
    77941 gtggtcaagg ccaggaactt tattcagtac ctggatatcc acgccttcat caggttgcag
    78001 acggatagtc agtttattct gcggcagatc ctgccacgac tctttaaaca gattcagttc
    78061 aggtgttttg aaatagacca cgacttcaga acatttggtc ggcagacgtt taccagtacg
    78121 caggtagaat ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga tcgccacgaa
    78181 ggtttctgta ttgctgctct tgttcgcgcc ctcttcttcc agatatcccg gcactttttt
    78241 gccctgggcg aagcccgcag tatattgccc acgtacggtt ttttcgcgta cgttggagcg
    78301 gtcgatgcgg cgcagggact tcagtacttt cactttttca tcgcggatgc tgtctgcgct
    78361 caggtcagat ggcggagaca tcgcaatcat gcagagaatt tgcagcaggt ggttttggat
    78421 catatcgcgc atctgaccgg ctttatcaaa atagccccag cgcccttcga tccccacttc
    78481 ttctgccacg gtaatctcaa catgatcaat ggtgcgattg tcccagttat tcacaaacag
    78541 ggagttagca aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa gatagtggtc
    78601 gatacggtaa acctggcact cctcgaagta ttcgccaacc tgatcattga tttcctgcga
    78661 ggtcgccagc gacgtcccca gcggtttctc catgactacg cgtgccggtt tagcattcag
    78721 ttttgcctcg ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca tggcaaagta
    78781 gttaatggtg atacgatttt tttgatccag catcgcgccg agacggttga atgcagcagt
    78841 gtcattgaca tcgagattac aaaaatccag acgtgcactc agggtgtccc ataaaccttc
    78901 atcaatggtt tctttcatga aagtttcgag cgcctcgcgg acaactttgg tatatgccgc
    78961 tttatcccag tcagcacgcc ctacgccgat aatccgggtg tccgggttga gctgaccggc
    79021 tttttccagt tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc ctttcgcgcc
    79081 gaaaatgacc aggtcacagg cctgggctgt ttgcgttacc gccatgtcat tctccttaag
    79141 ttaactaacc cggtacttaa gccagggtat acttgtaatt ttcttacggt gcactgtact
    79201 gcttttacga gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta cgggaaaagc
    79261 gcgcaaagtg cggcaaaact gataaaaaaa tcatcgtttt ccattgggta aaaatctgac
    79321 actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca ttaacacggc
    79381 acacaggcgt aatatctgac aaaactgcat ttcgatttct ttcagtgcgg aaatcgtcat
    79441 tacccgtgag tctctttaca tcatgaatat gctggaaaaa atccagtctc agctggaaca
    79501 tttgagcaaa tcagagcgca aagttgccga ggtcattctg gcttcgcccg ataacgcgat
    79561 ccattcgagt attgctgcta tggcactgga agccaatgtt agcgaaccga cggtgaatcg
    79621 tttctgtcgc agcatggaca cgcgcggttt tcctgatttt aaacttcatc tggcacagag
    79681 tctggcgaat ggcactccct atgttaatcg caacgtcaat gaagatgaca gcgttgaatc
    79741 atacacaggg aaaatatttg agtccgcaat ggcaacgctc gatcatgtcc gtcattcact
    79801 ggataaatct gccatcaacc gcgccgtcga cttgctcact caggcaaaaa aaatcgcctt
    79861 tttcggatta ggctcttcag ccgccgttgc ccacgatgcg atgaataagt tctttcgttt
    79921 taatgttccg gtggtgtact ccgatgatat tgtgctgcaa cgcatgagtt gtatgaattg
    79981 tagcgacgga gacgtggtgg tgctgatttc tcacactgga agaacaaaaa atctggtcga
    80041 gctggcgcag ctggctcgcg aaaacgacgc aatggtgatt gccctcacct ctgcgggtac
    80101 cccgctcgcc cgggaagcaa cgctggcaat taccctcgac gtaccggaag atactgacat
    80161 ttatatgccc atggtttctc gacttgcaca gctgaccgtg atagatgtgc tggcgacagg
    80221 atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca aggaagcgct
    80281 gaaggaatcg cgttttgata agcagttact taatttaagt gacgatcgct aaaaacgact
    80341 gtcactgtcc taatcttata cgacatccga atgagattaa tttatcgcca tcgcggcgtt
    80401 atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg gagtattaca
    80461 tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca acagatcgcg
    80521 ataataatct tgaaaaagtt atcgcggcgg gtgccaacgt tgtacgtatg aacttttctc
    80581 acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgagatt gccgcaaaac
    80641 tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaatccgt gtatccacct
    80701 ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac gccaacctgg
    80761 gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg cctgctgacg
    80821 tcgtgcctgg tgacatcctg ctgctggacg atggtcgcgt ccagttaaaa gtactggaag
    80881 ttcagggcat gaaagtgttc accgaagtca ccgtcggtgg tcccctctcc aacaataaag
    80941 gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa gacaaagcag
    81001 acattaagac tgcggcgttg attggcgtag attacctggc tgtctccttc ccacgctgtg
    81061 gcgaagatct gaactatgcc cgtcgcctgg cacgcgatgc aggatgtgat gcgaaaattg
    81121 ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat gacatcatcc
    81181 tcgcctctga cgtggtaatg gttgcacgtg gcgacctcgg tgtggaaatt ggcgacccgg
    81241 aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagctaaac cgagcggtaa
    81301 tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg cgtgcagaag
    81361 tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctg tctgcagaaa
    81421 ctgccgctgg gcagtatccg tcagaaaccg ttgcagccat ggcgcgcgtt tgcctgggtg
    81481 cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgttcag ttcgacaatg
    81541 tggaagaagc tattgccatg tcagcaatgt acgcagctaa ccacctgaaa ggcgttacgg
    81601 cgatcatcac catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt atcagctctg
    81661 gtctgccaat tttcgccatg tcgcgccatg aacgtacgct gaacctgact gctctctatc
    81721 gtggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcagct gccagcgaag
    81781 cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg attgtcaccc
    81841 agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta acggtagagt
    81901 aatctcacct accccggccg ctcatgaccg gggtatttag caacacataa attaggacta
    81961 cttattcaat ccaatcataa tcacagcccc ctgtaaaatg aatacaggga tagcctgaat
    82021 ggagatctaa atagcttgca agacgtcgca tacccgcttc ctctgcaaca ctatgtccaa
    82081 taactgccat cggtaagcca ctatctttgg agtattgtgt ctgcacccat gttttaattg
    82141 aatcgttaat cgtaatataa taatcagcct gatattccat atattgtatt ggatcacaaa
    82201 aacagcctgc cccaatgcca acagttctga ctactctggc tttatctcca taaaattgaa
    82261 tgccaggaag attatatatt ttcatatttt tggcaaaacg tttggtaatg ttaatggcac
    82321 tgtcaggttc tatagcatat acacagtaca tctcatcaga agcaacaata tttgtaacat
    82381 ccagttctaa ctgctgagcc aaagccttag acattccgaa cgtaggctct cgatccatga
    82441 catcatggca acgaatgatt accaaattat ttttatgcaa aaactccttt ttttgctcaa
    82501 ttatttttaa ataactgagt tcaccgcgag tataatccat tatttttctg gcccatgaaa
    82561 actcaagatc gtcaccctcc agatcagcat aagaataaaa tgtaggctca tgtgtaataa
    82621 tgatattgac accttcaaaa acagcctttc ttaaaacatc cattgaggca atccagcagg
    82681 tcccaatttt attaataagt gtcgcctcag caccaaaaat aattttatca acagttggtt
    82741 gcgaaacagc aaccattgaa tttaaaaaac aattaagatc cgcggctgtt ttaatatgat
    82801 gcattcaaca tccttcacag gtttcatcag caataaaata atttcaacat ttctgggaaa
    82861 cgttttgccc atgcagactc agagtgggta tcacctcccc ataaatgata atctatagtt
    82921 acattgcgtt gtttacggag actcacgcat aatttttcag caccggaaat atagacttca
    82981 ggaaagtctt cacgcatacc actggtttcc tgcgtaccga catcgagata tacagtaaga
    83041 tcttcaacga tgttgttttc ctgaataaaa ttcagcattg cgctgtcatt gaaccatagt
    83101 gccggcgaaa aaacgccaac ttttgaaaaa acgtcctgac gaagaattgc ggcaaacaaa
    83161 ctgatgtacg cacccataga actacctgct atcgctgtat tattacgatc tgggagagtt
    83221 ctaaaattac tgtcaatata gttcttgagc gtattcacaa taaaatcgac atacagcgcg
    83281 ccttttcctc cactataatc cacagcaggg tcccagccag gtaagggaaa agatgtgttt
    83341 cgcggccatg gagaatactc atccatacgg ctgaatttac catgagacga tgcgttatca
    83401 atccccacaa tgataacacc gtcgtattgt gaatttggga aaaaagcatc taccgcctca
    83461 ggtatatccc atacatgtgg accaaaagat acagatttat caaataaatt ttgcccatcg
    83521 tgcatatata atacagggta actgatatca gattcataat aggacgcagg cagtaatatt
    83581 cttatttcgc gactactcgc caattgtggc atcggaaagg attgcaacac atgatatcga
    83641 ttcccatact tctcaatatc gttcataatt taaattaacc tcaataaaca aaagaaatat
    83701 tacatctaag cactccccta ccacattata tatgggagca cttagaaatg actaatatct
    83761 atatttttat cttaaatagt tttttcgcat tcgtaaacat aagttgttca aactcacttt
    83821 gcgttaattt actttttaat tcagcaaaat acttattata aaaatcatca tcattgtagg
    83881 tatctagccc attaatagga ttatcggtac cgaataaaat cttatcacta ccacagattt
    83941 ttatcgcctg gtaaaccata tccgccctaa cccacgcagt atcgccatag agattaggaa
    84001 gttttgcaat caattcgatt gcttcctgat tatcagtcgc gagccccata tgacacatta
    84061 caaacttcac gtctggatat ttaagtgcca tctcataaac caactgtggg ctggattcat
    84121 aatcattaga agtatggcta actaccggga gatcatattt ttgagcaagg cgtatataat
    84181 cctgaacttt ttctgagttg aaagccattt ttgaatgata agggtgaact ttgatgccat
    84241 aaatgatatc tcgattgctg gcaatcatat cttcaaagtc ttgatcacaa ctttccagac
    84301 gaggcttaat ccacataaac gcgccaattc gctttgcatg ttgacgaaca agtctgagca
    84361 tacgctcatt attatcatgc tgtgtaactt gatcttcatc cggaatcggg ttctggtcat
    84421 ggtcaacttc aacggcagat ccacaagaac ataacgcaaa atcaatatta tacttgtcca
    84481 gagatgccag aaaaacatct tctggcatgc tgattttcac aaaatcacca atatgagtgt
    84541 gtgcatcaat tatcataaca aacgccctaa tagaaattta aaaatcataa agatcagcag
    84601 taggtaaccc ttcaatttca taactgaaac caggtatttc catacttctt ccgttataaa
    84661 taaagctggc ggtatcttca ctattattta agaagaaagt aatgttatcg cgctgataag
    84721 cgatcaccgt aggatgttca acgtctttcc attgtattga tgcctcacac cattgtggat
    84781 gttttttacg tagacttatc agccaacgta taaatttccg caattcaaga tcttgtttat
    84841 tttcctccca taccatacat tttctattat tctcaagcgt cattgattta taaccatcaa
    84901 tacctatttc gctaccgtgg tagatacaag gagaacccgc ttgagttaac ataaataaat
    84961 aggcgagttt aacttttcgt ttatcattca aacaaacgct taaaatacgc gcggtgtcat
    85021 gactatccag aagattaaac ataacttcag ttatattttt aggataacaa agataagaac
    85081 gtgttacgga tgtcataaat tcttgttttg tagtatcctg taacgcaaaa taatcaataa
    85141 tcccatatgt taagggatag ttcattaatg aatcaaactg atcaccacgc aaccaagggg
    85201 tgccctcatg ccatatctca cccaaaatat aacattcagg tttgatatct ttaatcacct
    85261 ttctgaaatc tcgccagaaa tgatgatcca cttcattcgc aacatccaga cgccaaccat
    85321 caatatcaaa attttgcgtc caatagcgaa ctatactaag taagtattca cggcactctt
    85381 cattctcagt atttagctta ggcatttcaa taacattgcc aaaggtttca tagttaaagt
    85441 ttttaaaatc cctttcactt tttggagtat cgggataaac agggaattta tgaatccaga
    85501 accaatcggc gtaacgtgaa ttggcaccat tgcgtacaac gtcaagccat attggagaat
    85561 cagctcctaa atgattaaat actgcatcca acataatttt tattccacgc ttatgcgctt
    85621 cttcaatcaa agtatggaaa gcaatattcc cccctaaatg tggatcaaca ctaaaatgat
    85681 ctatggtgtc atatttatgg ttggaagctg aggtaaaaat aggacaaaaa taaatcccat
    85741 taatacccag atcttccaga taatcaagtt tatcaataac cccccacaga tccccaccca
    85801 taaaattgaa tgaagtcggt gtactgcccc acggttctac cccttctggg gatatttcag
    85861 gtctgccatt acagaaacgg tctggaaaaa tttggtacca taccgtattt tttacccagg
    85921 agggcgtatt tagaacgtca attttattaa gataagggaa gcaaaaaaag ttatttaagc
    85981 ggcttaactc tcgctcttcg cactccggtg aactgatatc aacacatctt ttctcaccaa
    86041 aaagaaactt ctctccctct ttcccaaaaa gtataaaacc gtaacggcaa cggcgttttt
    86101 gaggtttgaa cactgcaaac cagtgatcat gatattcggt gacagcttct tgttccatct
    86161 cgatctcgtt gccgccaatc cagccgaatg ctctgtcccg gccatgttac cgccgtcaag
    86221 accaccttca gcccaattat atggatcgcc tatccagaga ataactttat caacttctcc
    86281 tcttaatgtg cgtaatcgta catgcaaagt ttcgttatca taggcatagc tatcttcact
    86341 ttttgctgag tgttttaacg aactaactgt aatcataaat actaatccct aattattcct
    86401 taacggcacc gctaacaagt ccagtagtta tatgcttctg tgtgaaaaca aataacaatg
    86461 tgataggtac tgcaactaaa agtgccccag cagcaaaaag cgtataattt tctgctgaat
    86521 tagaagcaat ccaactgaaa attccaatcg ccagggtcat tttatcctca ctacgaagca
    86581 ccaacgttgg caaaatgaag tccatccatg gtccagtgaa cgaaactaat ccaacgaaaa
    86641 cgagaatcgg cttagccaat ggcaatatta tctgaataaa aatagtcaga tgccctgctc
    86701 catctatttt agcggcctca tccaaagaag ttggaatagc atcaaaataa ccttttacca
    86761 accacgtcat gaagggcaat gaaccggtta cataaaccag aagcaaccca gtatatgtgt
    86821 ctatcaaatt ggcctttgat agcaggatat aaatggcagt cattgataaa aatgcaggaa
    86881 acatttgcaa tacaagaata ctcattaacg tattacgctt tccacgaaat cgataccgtg
    86941 aaaaaacgta ggcggtcatc gtcacaacga ccagtgaaat taacatgtta gcggttgcaa
    87001 gtatgaaagt gttcaaatac cactgcatat atggcgtatc cgtgaacaac gttacgtaat
    87061 gttcaaatgt aaactcgata ccactaaatg tggaactaaa tagattattc ccttttttaa
    87121 aagatgccat tatcgtccat atcactggcc caagcacaac aatggagttg cagattaaaa
    87181 aagataaact acgagcgtag atattttacg taaaaattta tccatagtta catccctaca
    87241 tcatctttaa atgatttcat ccgtctgaat tgccatattg ctataaccga taagaagata
    87301 aagattataa tagagatcac agacgctata tgatattgct ggaaattcaa tgtaagttta
    87361 taaatccagg taatgaaaat atctgtatgc ccagcaaatc tatattccgg atttgtcggg
    87421 ccaccttcag tcagaagata aattgcacca aaattattga agttgtatgc aaatgtcatt
    87481 accagagttg gcgcaacctg atgtaacacc atcggcaatg taatttcctt aaactgttgc
    87541 cacttagttg cgccatcgac ctcactagcc tcatacaaat ctcgggatat gttggttaat
    87601 gcacccgcga tcaatagcat aaaatacggt gcccctaccc aaacacaaac ggcaattact
    87661 gttatttttg catttaatgg aaccgacaga aaatcaactg aattaattcc aatactgttc
    87721 agaaaagagt taaccggacc aattccattt aaaagaagac ggaaaataag taacgttaca
    87781 aatgtgggta tggcatatgg cagtataaat attaatcgcc agacttttgc gaaacgtata
    87841 ttttttttag ccagtgctaa cgccagaata aagccaaaac tgaaggtaaa aatcgtcgcg
    87901 caaattgccc aaatgaatgt ccagagagca acacctagaa acgtatcaga ccaaatttta
    87961 aatttaaaca acataatgaa atttttaatc ccaacccaat caaccaggtt ctttggtggg
    88021 atgtgatttg gtgcagcata gtttgtaaac gatacaagaa ctgtaattac aataggtaaa
    88081 acaatgaatg ctatactcgc taaaaatgcg ggcgttagga ctataaatgc aaatttatcc
    88141 tcatagagtt tatataactg ttgtttaaaa gtcagatggc aataagatga gttccgcgca
    88201 tctttaatat taaggatata aatagtggcg aataagccaa caataattgt cgctatcacg
    88261 ccttctacaa gcataaatat agaattgtca ccctgaataa cagtcaaccc ttttcgaatt
    88321 tgcgcgacat cacctaatgt gaacaaacct attaatgtat gcacaatatc ggaaagataa
    88381 agcaaaaagc atacttgtat tgcaaagaat attccgcctt tcacataata atgacgtgat
    88441 aactgagtcc cacccataat caaacaggaa attggtagca caggtgcact cttcattttt
    88501 atttgttgag tattcacaat tactcctgaa gcgaaagttg ttcttcaata attgtatgtg
    88561 catggtttaa aacttcaccc ggtgttttat tctcaaccca ggtagccaaa atagcattag
    88621 tcattggaga ccatagataa gacatttccg gtattgatgg cattgcgtca gaataattag
    88681 cttgagcaat aattgctttt atagcttcac tacctgtctg gctaattttc ttcataagcg
    88741 aatcgactgg tgggatgaga ttcgtcattt cataacgctt catcaacatc ttatccgttg
    88801 tcaggtagtc agcgaataat tcagcagcat gaggatattc ggtaaatgag cttacaacgg
    88861 ccagccgtac tgttgaaaat gagcgaggtt gatgaccatc taatgttggg atgcgtgtta
    88921 cgccaaaatt gattccgctg tcaatgtaac cttgtatgga ccaaggtccg tcgattacag
    88981 cagcaacttt tccttcatta aacagacctc ttagaacttg tggattaccc atatcaagag
    89041 gattagaggg attcgctttc tttaagcgtt tcattgtctc tagcccttcc ttcgcttcag
    89101 atgaagcgat gcctaaagct ttgggatcat taccgctttt accgaattcg ttggcaccat
    89161 ataaggtaat aaacatacgt gaaacataat aattttgtac atcccacagc agagcatatt
    89221 tatgctcgga tgaattatta aacttttcac tgaaagtatt aatttcttcg aatgtttttg
    89281 gtgcggcgct taacaaatcc ttattataga aaagcgccag cgttgcgaaa cttacaggaa
    89341 aaccatagct gatgttatta tatgtagctg ccgatactgc acctggaata aatgttttct
    89401 taatccgatc agcggagacc atgttctcca taaccccgcc agcaacgact aaccgcccca
    89461 gggtatcatg ttcaatttca gctacatcag ctacacgagt cgtaccacca tcctgcataa
    89521 ttcttgatgc tgcatccatt ggcgctatat tgcgaaatgt aaactttacc ttgtagccaa
    89581 aatctttatt gaattccttt gctgcatatt tcatatattc cacgcttgtg gcatcagacc
    89641 aaactaacaa ctcagcatca gtttcaggaa tcaatttatc tgccatcgcc tggggtgtta
    89701 gcgtactgga aattgcaaga attataactg tgacgatatt tgaaagcttc attatattta
    89761 cctcgtgaat aacatcttta agtagtacat gctgtactcc tatataagca ttcacgccat
    89821 tatcaacaca aatcaaggcc aatcaactgt caaataagat tttaatgatg aataactgta
    89881 aatagtgatg catatcccat ttgcagtaat ttgttgagga aaaaaatatt ttattgccgc
    89941 agagtgttta ggagtacatg aggtacgact atatggaata gcagaaatca tattttaaat
    90001 tgatattttg gaaggaactc atttatggtt ttaataaaaa agaatgtctt atcggcttca
    90061 attgcaacag tccttttagg ctcaacagga gcctttgcgg caaacgatcg cggctttacc
    90121 tccgacactg actggtcaga agaacaagcg ggctttaatg gacatgttgg tactacattt
    90181 gaatatgaga ccaaaagcac tgataactat ttaggtaaaa cagtaaagga atatactaaa
    90241 acacacgaga ttttccaggc ctatttcagc caacctaatt ggcaatttgc agcaatctat
    90301 ggccttaagt ctatcgatcg ccggcaagaa gaaaaaggct atcacgaaaa tgaaacaagt
    90361 ctgcaaaact atatatcact aaacaaaacg ttcactattg gtaacggttt cgacttcagt
    90421 ctggtatacg acctgatgta taccgacgga aatatagata gcccttatgt tacaggcttg
    90481 catactgtaa ctgttgacaa cagttttcgc cctacgttga cgtactttaa ctctgactgg
    90541 aacgcaggtt tttattcaaa cgttgaatac ttatacagtc gaaatgataa gagttcctgg
    90601 ggggtacgcg aagaagaagg tcaaagtata ctatttcaac cctataagcg ttttggagcc
    90661 tgggaatttg gcattgagtt tttctatcag aaaaagcata ccgatgaatt taatcacggt
    90721 aaaataaacg atcgtggtat ttataccgaa acttactacg agccaatcat caaatatgct
    90781 tttgaaaatg caggtaatat ttatcttcgc actcgcttcg gacacgggaa aacagaaaac
    90841 gcagatagtt tgcaatacaa tgcaaacaga acctatttca aaacaattcg aaaagccact
    90901 ctaggttatg aacaaaacat aggtgacgac tggatagcca agattgaata taaacatgcc
    90961 tgggaaaaag agcacaaaaa cttttatgat ccaagagatc aagaaaaata taatgtgctc
    91021 aatcaggata atatttatgt tcatgctttc tatcgctttt gattaaacag agggaaacct
    91081 atgttcagcg cactagatat taaaacgttg atgcaaggaa ctttatatgg tgatccatca
    91141 ctcagggttg ataccattcg gccaatccat agtcctttag ttggtggact ctcgattgtt
    91201 atgacacctg gagatttgtt gcatatacca acgactggtg cggatataat tatcggaccg
    91261 gaagagatca ttagttctaa cgctaaggca aagattgtag tcgattatct gaatgtgaat
    91321 aaccttaaca aagttttaag atactataag gtacataagt atcgtttgtt tgagcaagaa
    91381 aacacttcaa cgatacctga tgtatacatt ggtaaacact gccagatagg tatgaactgc
    91441 cattttatgc ctggtgttaa aattatgaac tgtgtgacca taggtgataa tgttgccatt
    91501 catgcgaata cagtgattaa agaaggcact attattggca atgatgttat catagattct
    91561 aataactcca tcggtaatta tagttttgaa tatatgtcgg atgaacgtga ttcatatgtc
    91621 cgagttgata gcatcggtag agttatcata ggtgatgatg ttgaaatcgg ttgtaacaac
    91681 accattgacc gagggacact tggagatacg attataggca aaggcactcg tattgataat
    91741 caagtgcaga ttggacatga ctgcataatc ggtaataaat gcctgattgt ttcacaatgc
    91801 ggtttttcag gacacgtagt tttaggcgac catgttatta cacatggtca agtcggaatc
    91861 gccggtcaca tatcaattgg tagttattcg gtaattaagg ctaaatcagg tgtaagccat
    91921 tcttgtcctg aaaagagtga cctttttggt taccctgcca aaaatacacg agaatacaac
    91981 aaaaatctcg ctgttcttaa taaactaact aaacaaca
//
LOCUS       DAOFQD010000002        86299 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.002, whole genome shotgun sequence.
ACCESSION   DAOFQD010000002 DAOFQD010000000
VERSION     DAOFQD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.002"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            174..341
                     /locus_tag="QBF46_000096"
     CDS             174..341
                     /locus_tag="QBF46_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDQ6672340.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            584..1972
                     /gene="frlA"
                     /locus_tag="QBF46_000097"
     CDS             584..1972
                     /gene="frlA"
                     /locus_tag="QBF46_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HDQ6672341.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            1993..3015
                     /gene="frlB"
                     /locus_tag="QBF46_000098"
     CDS             1993..3015
                     /gene="frlB"
                     /locus_tag="QBF46_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HDQ6672342.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGEQASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            3066..3893
                     /gene="frlC"
                     /locus_tag="QBF46_000099"
     CDS             3066..3893
                     /gene="frlC"
                     /locus_tag="QBF46_000099"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HDQ6672343.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            3893..4678
                     /gene="frlD"
                     /locus_tag="QBF46_000100"
     CDS             3893..4678
                     /gene="frlD"
                     /locus_tag="QBF46_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDQ6672344.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIKPGCI
                     TWVGDDDYGTKLKQDLACMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPELVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4778..5509
                     /gene="phoB"
                     /locus_tag="QBF46_000101"
     CDS             4778..5509
                     /gene="phoB"
                     /locus_tag="QBF46_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HDQ6672345.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5617..6702)
                     /locus_tag="QBF46_000102"
     CDS             complement(5617..6702)
                     /locus_tag="QBF46_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDQ6672346.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPVLTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6714..8018)
                     /locus_tag="QBF46_000103"
     CDS             complement(6714..8018)
                     /locus_tag="QBF46_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDQ6672347.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(8030..8383)
                     /locus_tag="QBF46_000104"
     CDS             complement(8030..8383)
                     /locus_tag="QBF46_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDQ6672348.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8394..9272)
                     /locus_tag="QBF46_000105"
     CDS             complement(8394..9272)
                     /locus_tag="QBF46_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDQ6672349.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(9269..10495)
                     /locus_tag="QBF46_000106"
     CDS             complement(9269..10495)
                     /locus_tag="QBF46_000106"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDQ6672350.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10495..11658)
                     /locus_tag="QBF46_000107"
     CDS             complement(10495..11658)
                     /locus_tag="QBF46_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDQ6672351.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFALKVLPEIVAEIQNLPGLHLAGLTHFPCLLWDEAVGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(11742..12104)
                     /locus_tag="QBF46_000108"
     CDS             complement(11742..12104)
                     /locus_tag="QBF46_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDQ6672352.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(12121..13026)
                     /locus_tag="QBF46_000109"
     CDS             complement(12121..13026)
                     /locus_tag="QBF46_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672353.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13295..14299)
                     /gene="trpS"
                     /locus_tag="QBF46_000110"
     CDS             complement(13295..14299)
                     /gene="trpS"
                     /locus_tag="QBF46_000110"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDQ6672354.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14292..15050)
                     /gene="gph"
                     /locus_tag="QBF46_000111"
     CDS             complement(14292..15050)
                     /gene="gph"
                     /locus_tag="QBF46_000111"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDQ6672355.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(15043..15720)
                     /gene="rpe"
                     /locus_tag="QBF46_000112"
     CDS             complement(15043..15720)
                     /gene="rpe"
                     /locus_tag="QBF46_000112"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDQ6672356.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15738..16574)
                     /gene="dam"
                     /locus_tag="QBF46_000113"
     CDS             complement(15738..16574)
                     /gene="dam"
                     /locus_tag="QBF46_000113"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDQ6672357.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16681..17967)
                     /gene="damX"
                     /locus_tag="QBF46_000114"
     CDS             complement(16681..17967)
                     /gene="damX"
                     /locus_tag="QBF46_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDQ6672358.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKSQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(18059..19147)
                     /gene="aroB"
                     /locus_tag="QBF46_000115"
     CDS             complement(18059..19147)
                     /gene="aroB"
                     /locus_tag="QBF46_000115"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDQ6672359.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19204..19725)
                     /gene="aroK"
                     /locus_tag="QBF46_000116"
     CDS             complement(19204..19725)
                     /gene="aroK"
                     /locus_tag="QBF46_000116"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDQ6672360.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(20126..21364)
                     /gene="hofQ"
                     /locus_tag="QBF46_000117"
     CDS             complement(20126..21364)
                     /gene="hofQ"
                     /locus_tag="QBF46_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDQ6672361.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21276..21680)
                     /gene="hofP"
                     /locus_tag="QBF46_000118"
     CDS             complement(21276..21680)
                     /gene="hofP"
                     /locus_tag="QBF46_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDQ6672362.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21670..22110)
                     /gene="hofO"
                     /locus_tag="QBF46_000119"
     CDS             complement(21670..22110)
                     /gene="hofO"
                     /locus_tag="QBF46_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDQ6672363.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELVLK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(22094..22633)
                     /gene="hofN"
                     /locus_tag="QBF46_000120"
     CDS             complement(22094..22633)
                     /gene="hofN"
                     /locus_tag="QBF46_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDQ6672364.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22633..23412)
                     /gene="hofM"
                     /locus_tag="QBF46_000121"
     CDS             complement(22633..23412)
                     /gene="hofM"
                     /locus_tag="QBF46_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDQ6672365.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23532..26084
                     /gene="mrcA"
                     /locus_tag="QBF46_000122"
     CDS             23532..26084
                     /gene="mrcA"
                     /locus_tag="QBF46_000122"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDQ6672366.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26249..26809)
                     /gene="nudE"
                     /locus_tag="QBF46_000123"
     CDS             complement(26249..26809)
                     /gene="nudE"
                     /locus_tag="QBF46_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDQ6672367.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            27129..29264
                     /gene="igaA"
                     /locus_tag="QBF46_000124"
     CDS             27129..29264
                     /gene="igaA"
                     /locus_tag="QBF46_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDQ6672368.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29329..29997
                     /gene="yrfG"
                     /locus_tag="QBF46_000125"
     CDS             29329..29997
                     /gene="yrfG"
                     /locus_tag="QBF46_000125"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDQ6672369.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            30008..30409
                     /gene="hslR"
                     /locus_tag="QBF46_000126"
     CDS             30008..30409
                     /gene="hslR"
                     /locus_tag="QBF46_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDQ6672370.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30434..31312
                     /gene="hslO"
                     /locus_tag="QBF46_000127"
     CDS             30434..31312
                     /gene="hslO"
                     /locus_tag="QBF46_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDQ6672371.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31375..33099)
                     /locus_tag="QBF46_000128"
     CDS             complement(31375..33099)
                     /locus_tag="QBF46_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDQ6672372.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIVWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQVSEKV
                     LADNVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLNVNLNE"
     gene            33478..35100
                     /gene="pckA"
                     /locus_tag="QBF46_000129"
     CDS             33478..35100
                     /gene="pckA"
                     /locus_tag="QBF46_000129"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDQ6672373.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            35217..35534
                     /locus_tag="QBF46_000130"
     CDS             35217..35534
                     /locus_tag="QBF46_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDQ6672374.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRIFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            35593..35889
                     /locus_tag="QBF46_000131"
     CDS             35593..35889
                     /locus_tag="QBF46_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6672375.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(35920..37272)
                     /gene="envZ"
                     /locus_tag="QBF46_000132"
     CDS             complement(35920..37272)
                     /gene="envZ"
                     /locus_tag="QBF46_000132"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDQ6672376.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(37269..37988)
                     /gene="ompR"
                     /locus_tag="QBF46_000133"
     CDS             complement(37269..37988)
                     /gene="ompR"
                     /locus_tag="QBF46_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDQ6672377.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            38216..38692
                     /gene="greB"
                     /locus_tag="QBF46_000134"
     CDS             38216..38692
                     /gene="greB"
                     /locus_tag="QBF46_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDQ6672378.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            38789..41110
                     /locus_tag="QBF46_000135"
     CDS             38789..41110
                     /locus_tag="QBF46_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDQ6672379.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            41548..41775
                     /gene="feoA"
                     /locus_tag="QBF46_000136"
     CDS             41548..41775
                     /gene="feoA"
                     /locus_tag="QBF46_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDQ6672380.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            41792..44113
                     /gene="feoB"
                     /locus_tag="QBF46_000137"
     CDS             41792..44113
                     /gene="feoB"
                     /locus_tag="QBF46_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDQ6672381.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            44113..44349
                     /gene="feoC"
                     /locus_tag="QBF46_000138"
     CDS             44113..44349
                     /gene="feoC"
                     /locus_tag="QBF46_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDQ6672382.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            44552..45430
                     /gene="rpnA"
                     /locus_tag="QBF46_000139"
     CDS             44552..45430
                     /gene="rpnA"
                     /locus_tag="QBF46_000139"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDQ6672383.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLVRQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(45457..46227)
                     /gene="bioH"
                     /locus_tag="QBF46_000140"
     CDS             complement(45457..46227)
                     /gene="bioH"
                     /locus_tag="QBF46_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDQ6672384.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            46265..46948
                     /gene="gntX"
                     /locus_tag="QBF46_000141"
     CDS             46265..46948
                     /gene="gntX"
                     /locus_tag="QBF46_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDQ6672385.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            47007..47582
                     /gene="nfuA"
                     /locus_tag="QBF46_000142"
     CDS             47007..47582
                     /gene="nfuA"
                     /locus_tag="QBF46_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDQ6672386.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47943..49259
                     /gene="gntT"
                     /locus_tag="QBF46_000143"
     CDS             47943..49259
                     /gene="gntT"
                     /locus_tag="QBF46_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDQ6672387.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(49370..51454)
                     /gene="malQ"
                     /locus_tag="QBF46_000144"
     CDS             complement(49370..51454)
                     /gene="malQ"
                     /locus_tag="QBF46_000144"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDQ6672388.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(51464..53857)
                     /gene="malP"
                     /locus_tag="QBF46_000145"
     CDS             complement(51464..53857)
                     /gene="malP"
                     /locus_tag="QBF46_000145"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDQ6672389.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWEVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            54469..57174
                     /gene="malT"
                     /locus_tag="QBF46_000146"
     CDS             54469..57174
                     /gene="malT"
                     /locus_tag="QBF46_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDQ6672390.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQDAVQHAQQLLKMMGYGV"
     gene            complement(57217..58233)
                     /gene="rtcA"
                     /locus_tag="QBF46_000147"
     CDS             complement(57217..58233)
                     /gene="rtcA"
                     /locus_tag="QBF46_000147"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDQ6672391.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     TAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTR
                     VSIE"
     gene            complement(58237..59463)
                     /gene="rtcB"
                     /locus_tag="QBF46_000148"
     CDS             complement(58237..59463)
                     /gene="rtcB"
                     /locus_tag="QBF46_000148"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDQ6672392.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            59652..61250
                     /gene="rtcR"
                     /locus_tag="QBF46_000149"
     CDS             59652..61250
                     /gene="rtcR"
                     /locus_tag="QBF46_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDQ6672393.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWLPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDITSVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(61232..61990)
                     /locus_tag="QBF46_000150"
     CDS             complement(61232..61990)
                     /locus_tag="QBF46_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDQ6672394.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAQPPVSVMQVLTDHHIQLELC"
     gene            complement(62007..62837)
                     /gene="glpG"
                     /locus_tag="QBF46_000151"
     CDS             complement(62007..62837)
                     /gene="glpG"
                     /locus_tag="QBF46_000151"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDQ6672395.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(62882..63208)
                     /gene="glpE"
                     /locus_tag="QBF46_000152"
     CDS             complement(62882..63208)
                     /gene="glpE"
                     /locus_tag="QBF46_000152"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDQ6672396.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            63398..64903
                     /gene="glpD"
                     /locus_tag="QBF46_000153"
     CDS             63398..64903
                     /gene="glpD"
                     /locus_tag="QBF46_000153"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDQ6672397.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(64955..65560)
                     /locus_tag="QBF46_000154"
     CDS             complement(64955..65560)
                     /locus_tag="QBF46_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672398.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(65563..66330)
                     /locus_tag="QBF46_000155"
     CDS             complement(65563..66330)
                     /locus_tag="QBF46_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672399.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDETVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERAWQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(66333..67043)
                     /locus_tag="QBF46_000156"
     CDS             complement(66333..67043)
                     /locus_tag="QBF46_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672400.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDHY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESRIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQLFTRDQRARG"
     gene            complement(67058..68566)
                     /locus_tag="QBF46_000157"
     CDS             complement(67058..68566)
                     /locus_tag="QBF46_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDQ6672401.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NILITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIHFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(68691..71138)
                     /gene="glgP"
                     /locus_tag="QBF46_000158"
     CDS             complement(68691..71138)
                     /gene="glgP"
                     /locus_tag="QBF46_000158"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDQ6672402.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(71157..72590)
                     /gene="glgA"
                     /locus_tag="QBF46_000159"
     CDS             complement(71157..72590)
                     /gene="glgA"
                     /locus_tag="QBF46_000159"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDQ6672403.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(72590..73885)
                     /gene="glgC"
                     /locus_tag="QBF46_000160"
     CDS             complement(72590..73885)
                     /gene="glgC"
                     /locus_tag="QBF46_000160"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDQ6672404.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(73903..75876)
                     /gene="glgX"
                     /locus_tag="QBF46_000161"
     CDS             complement(73903..75876)
                     /gene="glgX"
                     /locus_tag="QBF46_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDQ6672405.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRISALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(75873..78059)
                     /gene="glgB"
                     /locus_tag="QBF46_000162"
     CDS             complement(75873..78059)
                     /gene="glgB"
                     /locus_tag="QBF46_000162"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDQ6672406.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(78332..79435)
                     /gene="asd"
                     /locus_tag="QBF46_000163"
     CDS             complement(78332..79435)
                     /gene="asd"
                     /locus_tag="QBF46_000163"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDQ6672407.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            79628..80221
                     /gene="yhgN"
                     /locus_tag="QBF46_000164"
     CDS             79628..80221
                     /gene="yhgN"
                     /locus_tag="QBF46_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDQ6672408.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(80278..81618)
                     /gene="gntU"
                     /locus_tag="QBF46_000165"
     CDS             complement(80278..81618)
                     /gene="gntU"
                     /locus_tag="QBF46_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDQ6672409.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(81622..82149)
                     /gene="gntK"
                     /locus_tag="QBF46_000166"
     CDS             complement(81622..82149)
                     /gene="gntK"
                     /locus_tag="QBF46_000166"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDQ6672410.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(82288..83283)
                     /gene="gntR"
                     /locus_tag="QBF46_000167"
     CDS             complement(82288..83283)
                     /gene="gntR"
                     /locus_tag="QBF46_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDQ6672411.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(83507..84202)
                     /gene="yhhW"
                     /locus_tag="QBF46_000168"
     CDS             complement(83507..84202)
                     /gene="yhhW"
                     /locus_tag="QBF46_000168"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDQ6672412.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(84325..85362)
                     /locus_tag="QBF46_000169"
     CDS             complement(84325..85362)
                     /locus_tag="QBF46_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDQ6672413.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            85695..86183
                     /gene="yhhY"
                     /locus_tag="QBF46_000170"
     CDS             85695..86183
                     /gene="yhhY"
                     /locus_tag="QBF46_000170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ6672414.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        20890 a        23491 c        21661 g        20257 t
ORIGIN      
        1 acaaaaatca tttgtttttg ccccgttata ttcaccgtta cagcgactta caaaattaag
       61 tcattcactc actgacgccg attaaatatt cctgtatcgt tcttaatgag cagtctactt
      121 atattcatat aatcaatgaa tattaatcaa tatataatac atagggaatg taaatgaaca
      181 aatttattaa agttgcactg gtaggtgcgg tactggctac gttaactgca tgtactggtc
      241 atattgaaaa ccgtgataag aactgctctt acgactacct gctgcaccct gcaatttcta
      301 tttctaaaat cattggcggt tgcggtccta ctgcacagta agtctctgac agaaatcggc
      361 taacaccggt ttaattgccc gacgccctct ggattttccg agggcgtatt tgtttctatg
      421 tccttcttgc cccgcccgta aaaatatttt cactttaaat tcaatttgat aactacatcc
      481 attagccaca gcgcgatcct gtgcgaaatt ttgtgatctt cctccacatt acataacatc
      541 atatgttgtt atattcatca tgcattgtca tgttaccttt taaatgactg caaactctcc
      601 cctacaacgt attggacaag aaaaaggtat cgctatggga agccaggaac tccaacgcaa
      661 gctcggattt tgggccgttc ttgcaatcgc cgtcgggaca accgtcggct ccggtatttt
      721 tgtatctgtg ggtgaagtgg caaaagcagc gggcacgccg tggcttacgg tgctcgcgtt
      781 tgtcattggc gggttaattg tgatcccgca aatgtgcgtc tatgcggaac tatccaccgc
      841 ttatccggaa aatggcgcag attatgttta tctgaaaaat gccggaagcc gaccgctggc
      901 tttcctctcc ggctgggcca gcttctgggc caacgatgcg ccgtcattgt cgattatggc
      961 gctggcgatt gtcagcaatc ttggcttttt aacgcctatc gatccgttgc tcggtaaatt
     1021 tatcgccgcc ggattaatta tcgcctttat gttgctacac ctgcgctccg ttgaaggcgg
     1081 cgcagcgttt cagacgctaa ttaccatcgc caaaattatc ccgttcacta tcgtcattgg
     1141 ccttgggatc ttctggttta aagcggagaa ttttgccgcc cctaccacca ctgcgattgg
     1201 cgcaacgggc agctttatgg cgctgctggc ggggatctct gccaccagtt ggtcgtatac
     1261 cggcatggcc tctatctgtt atatgaccgg cgaaattaaa aaccccggaa aaaccatgcc
     1321 acgagcgctg attggttcct gtctgctggt tctggtgctc tacaccctgc tggcgctggt
     1381 gatttccggc ctgatgccct tcgacaaact cgccaattct gaaacgccga tttccgacgc
     1441 cctgacctgg atccccgcac tcggcagcac cgctgggatc tttgttgcca tcacggcgat
     1501 gatcgtcatt ctcggatcgc tttccagctg cgtgatgtac cagccgcggc tggaatacgc
     1561 gatggcgaaa gacaacctgt tcttcaaatg cttcggccat gtgcatccga aatacaacac
     1621 gccggatgtc tccatcattt tgcaaggggc gctggggatc ttcttcatct tcgtttccga
     1681 tctcaccagc ctgctgggct atttcaccct ggtaatgtgt ttcaaaaata ccctcacctt
     1741 cggctccatc atctggtgtc gtaaacgcga cgattacaaa ccgctgtggc gtactccggc
     1801 tttcgggctg atgaccaccc tcgccattgc gtcaagcctc attctggtcg catcaacctt
     1861 tgtctgggca ccgattcccg gccttatctg cgccgtcatc gttattgcca ctggtctgcc
     1921 tgcttacgcc ttctgggcga agcgtagccg ccagctcaac gctttgtcgt aatgttatct
     1981 ggagaaaata aaatgttgga tattgataaa agcaccgtgg actttctggt caccgaaaat
     2041 atggttcagg aagtggaaaa agttctcagc catgacgttc cgctggtgca caccatcgtg
     2101 gaagagatgg tgaagcgcga catcgatcgt atttatttcg ttgcctgcgg atcgccactc
     2161 aacgcggcgc aaacggcgaa acatctggca gatcgctttt ccgatcttca ggtttacgcc
     2221 atttccggct gggagttctg cgataacacc ccgtatcgcc tcgacgatcg ttgcgcggtg
     2281 attggcgttt ctgactacgg taaaaccgaa gaggtaatca aagcgctgga gctgggccgg
     2341 gcctgcggcg cactcactgc ggcgttcacc aaacgcgcgg acagcccgat tacctcggcg
     2401 gcggaattta gcattgatta tcaggccgac tgtatctggg aaattcatct gctgctctgc
     2461 tacagcgtgg tgctggagat gatcacccgc ctcgcgccgc acgcggaaat cggcaagatc
     2521 aaaaacgatc tcaagcagct gccaaatgcg ctcggtcatc tggtacgcac ctgggaagag
     2581 aaaggtcgcc agctcggtga acaggccagt cagtggccga tgatttacac ggttgccgca
     2641 ggcccgctgc gcccgctggg ttacaaagaa ggcatcgtga cgctgatgga atttacctgg
     2701 acgcacggct gtgtgattga gagcggtgag ttccgtcacg gcccgctgga gattgtcgaa
     2761 ccgggcgttc cgttcctgtt tctgctcggc aatgatgaaa gtcgccacac caccgaacgc
     2821 gccattaact ttgttaaaca gcgtactgac aacgtgatcg tcatcgatta cgccgaaatt
     2881 tcgcaagggc tgcacccgtg gctggcaccg ttcctgatgt tcgtgccaat ggagtggctc
     2941 tgctactacc tgtctattta caaagatcac aacccggatg aacgccgcta ttacggtggt
     3001 ctggtggaat attaatcctc tctcccggcc cggcaacggg ccggatttac gcaaggagtt
     3061 acccgatgaa aacaggtatg tttacctgcg gccaccagcg gctgcctatt gaacacgcat
     3121 tccgtgatgc aagcgagctg ggttacgatg gcatcgaaat ttggggcggc cgcccgcacg
     3181 cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt
     3241 atcagatgcc gattatcggt tacacgccag aaaccaacgg ctatccgtat aacatgatgc
     3301 tgggcgatga acatatgcgt cgtgaaagcc tggacatgat caagctggcg atggatatgg
     3361 caaaagagat gaacgcgggt tatacgctga tttctgcggc ccacgcgggc tatctcacgc
     3421 cgcctaacgt tatctggggg cggctggcag agaacttaag cgaactgtgt gagtacgcgg
     3481 aaaacatcgg catggatctg atcctcgaac cgttaacgcc gtatgaatcg aacgtcgtgt
     3541 gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc ttcgccgcgc ctgttcagca
     3601 tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt gatgagttat ttcgacaaac
     3661 tgggcgataa attacgtcat ctgcatattg tcgacagcga cggggccagc gacacgcatt
     3721 acattcccgg cgaaggcaaa atgccgctgc gggaactgat gcgcgatatt attgatcggg
     3781 gctatgaggg atactgcaca gtggaactgg taacgatgta tatgaacgag cccagacttt
     3841 acgcccgcca ggcgctggaa cgctttcgcg cgctgctgcc ggaggatgag tgatgaaaac
     3901 cctggcgaca atcggcgata actgcgtcga tatctacccg caactgaata aagcgttttc
     3961 tggcggtaat gcggtgaacg tggcggtgta ctgcactcgc tacggtataa agccgggatg
     4021 cattacctgg gtgggtgacg atgactacgg cacaaagctt aagcaggatc tcgcctgcat
     4081 gggcgtcgat atcagccatg tccatacgaa acacggcgtt accgcacaaa ctcaggtgga
     4141 actgcacgac aatgatcgcg tttttggcga ctacaccgaa ggcgtgatgg ccgactttgc
     4201 cctgagtgaa gaggattacg cctggctggc gcagtatgac attgtgcacg cggcaatctg
     4261 gggacatgcg gaagacgcat tcccacagct gcacgctgcg ggcaaactta ccgctttcga
     4321 cttctccgac aagtgggaca gcccgctctg gcagacactg gtgccgcatc tcgattttgc
     4381 ctttgcctct gcaccgcaag aagacgaagc gctgcgtctg aagatgaaag cgattgttgc
     4441 ccgtggcgca ggaacagtga ttgtcacgct gggcgaaaac ggcagcattg cctgggatgg
     4501 cgcgcagttc tggcgtcagg ctcctgaact ggtgacggtt atcgacacca tgggtgccgg
     4561 agattcgttc attgccggat tcctttgcgg ctggtctgcg gggatgacat taccgcaggc
     4621 gatggcgcag ggaacggcgt gcgcggcgaa aaccattcag taccacggtg cctggtaggt
     4681 ataacgttgg cgtgagcatc ttcacgccaa cgtgctgtta cttgccggaa aacgacccta
     4741 taatccgagt aattcattct ttatttcagg gtcgattatg tcagctacgg accgctactc
     4801 tcatcaactc ctctacgcta ccgtccgcca gcggttgctg gatgatatcg cgcagggggt
     4861 ttaccaggcc gggcaacaga tccctaccga aaacgagctt tgtacgcaat ataacgtcag
     4921 ccgcattact attcgcaaag ctatcagcga cttagtggca gacggcgtac tgatccgctg
     4981 gcagggcaaa ggcacctttg tacaaagcca gaaggtcgaa aacgccctgc ttactgtcag
     5041 cggttttacc gattttggcg tctcacaagg caaggcgacg aaagagaaag tgatcgagca
     5101 ggaacggatc agcgccgcgc cgttttgcga aaagctgaat atccccggta acagcgaagt
     5161 gttccatctc tgccgggtga tgtatctcga taaagagccg ctgtttattg atagttcctg
     5221 gatcccgctg tcgcgttatc ctgactttga tgagatttac gtcgaaggaa gctccaccta
     5281 tcagttattt caggagcgtt ttgacacgcg agtggtcagc gacaaaaaga ccatcgatat
     5341 ctttgccgcc acccgcccgc aggcaaaatg gctgaaatgc gaactgggcg aaccgttgtt
     5401 tcgcatcagc aaaatcgcct ttgatcagaa tgacaaaccg gtgcacgtct ccgaactctt
     5461 ctgccgcgcc aatcgcatca ccttaacgat tgataataaa agacattaac cgtaggccgg
     5521 ataagatgcg ccagcatcgc atccggcgat gctggcgcgt tgaggggacc gtccggcgct
     5581 gaatttagct tgattgccga acggggctat ccagcattaa atactggcaa tactctcacg
     5641 caaaatccgc agcaccgtct cttcaccgct gcgattcggg ttaatgcgaa tcgcgcaatg
     5701 ttcgagctgt ggattcgcct ggcgaaacgt cccggaaagt cgataaaaaa gtggcgggat
     5761 ttcatatttc gactctgcac cgaccgggta aggtaaggca ccgcgtttct gcgcttcttc
     5821 cagcaccctg gcggcaatcg gctgatgaaa ctccacaatc aataccttcg actgcgcatt
     5881 agcaatgacg gcgcttttca cttccggcac tgcaccaccg ttgagcaaag ccagcaaccg
     5941 ttcagatacc cccgcctgca ccgcgtgcat cactggcgca aacaccagac cacgcaagac
     6001 ttccagcgcc tgagcgccct ggatctggct accgccggag taaagcgtgg cgcgaatacg
     6061 gttgataaca tcagcatcgc cgaccactgc accaacaccc tctggcccaa atagcttgaa
     6121 gcaggaaaat gtcgagacat tcgcgccgca ttcacagccg attcgcgcca ccttcatcac
     6181 cgcatagttg tcatcggtta acactggaac acctgccgcg cgcaacgttg ccagcacatc
     6241 tgccagcacg tagctgtcct gcggctgctg gcgcgtatgc tgcaccagcg ccgcatccgg
     6301 ttgttgctcg tcgacgacct gcttcagtgc cgacaggtca ttgaaatcaa cagtaataag
     6361 cgtcagcccc atctgctcaa taataacccg tgtcgtcggg taaacaggcg cgtcatgcac
     6421 cagaagacgc tgccccggtt tgagcaaagc cgccagcccg gcgcgaatcg cgccagtccc
     6481 cgcgccctgc accagcgccg cagcctgtgc gtgaaatgca tcagccagca cctgttccac
     6541 acgctgggta acgcgcggtt gattcagtcc tggcgttaag cctaaatcac cgccagtaag
     6601 aaactcgctg ccggggaaat ggcgacaaat gctatccacc agcgcaaact gcttttgctg
     6661 cgcctcaata atcgtcaggc tttgcagagg aaacgtcttc ataggtgcct tccttaagcc
     6721 ggaacgaaca ggccaagcca gtaaagaaca ttcagcagaa taccggtgat cattactgcc
     6781 acgaccggtg ccgccatttt ctgtaccgga cggcccaggg attcgttgag gaagtaaatg
     6841 gcaactgcga tagaaaatcc ggtataaccc gccatcttaa ttgctgcgaa aatcgaaccg
     6901 accagcaacg ccacttccat cagcatattc atggcgttac ggatgttatc cgacgcatta
     6961 cgcaccgacg ggtagcgccc cagccatttg ccgatcgaac gaagtaacag gacttctgcc
     7021 gaaataacca ctgcgcctaa taccgccgca accatcggat tcggcgagag atagcccacc
     7081 gcataaacaa aggtaaagcc cgcaactgca tatacgccgg tggctaacgc ggtggtggca
     7141 atcaacggca caaaacccaa tccgcgcata aattccgcca gagctgcctg gttgatcagc
     7201 gtttgcgatt gttccggcgt tacgcctgcg gagtacgctt tctccagagt gaagatcgac
     7261 acttcactgc cggcgaaaat cttcatactg gcaacggcgg caatcaatgc tcccacgatg
     7321 gcgatatagg gtaagttttt gataatccgc gacgtgcgtt cttcaaacac cgaaagcccg
     7381 ctggcatcga tgtcattttc atcacgatga cgcaggtcgt gagttatcgc gatccccagc
     7441 agcatcacca tgccaataaa gatttcgatg gattccggat tgagatgcgg gaaatagcgc
     7501 acgactacca cacgggtcat cagcacaacc acggcggcga tcagactttg cttccagcca
     7561 aactggtaga aaatcgccac caacgggaac aatgcaaaag ctgaaaccac cggcgagctt
     7621 aattccccga ggctacctaa tacatccacc ggcagcgcgg tcagcagctg gtttactggc
     7681 aacagacaag taaggatcaa cacgccccag atagcgccaa gaccaaacgc catcaggctg
     7741 tttatcgcca gtacgccgag gatatcggta ggaagaaaga gtaaccatgc gttgagcagc
     7801 ccggttttca gggtaaaaga gatccccacc gaggccacaa aaccaatgct cagaccaaaa
     7861 gcgatactcc ccgcctcgcg acggttcata tagccttcaa tcagttgcgg caggatcggg
     7921 cggatgccgt catgaaaaac agccgccgag cgatgcgcca gaagcgatgt catacccgtc
     7981 aggcacgcca ccacgataat ctgaatatac agatccatag tcgtgccttt tatttcagat
     8041 ggttaatcag catcggaatc gcgtgttcga cgtgctcaac ggaaaggcca aacgccactt
     8101 tcccttcggc gatcattttg gcgatatgct cgtctttcgc tttaatgccc ggcttggcaa
     8161 tagtgcaact tttgttatag ccaatcaccg cgatggcaat cgacaatgcc gcgcccgcac
     8221 cggtattaca cgcgccgatg taataatcca gttgcccgga tttcaccttc attgccgctt
     8281 ccatgtcgtt gtgaatgaaa acttcaaaac agccaggagc cgtcgcttca atggtttttt
     8341 taatctgctc acgctgtaag cctgcaacgc caatcttttt catgagtctg tccttattgg
     8401 aaaaattgag agggagtttc acgtaacatc acatccacat cggcctgact gaatcccgac
     8461 tggcgcaatt gcggaataaa ggtggtcaat aaatagtcat agccataacc accgttggct
     8521 tttaaatggg agcggcgcgt aatatccatc gacagcatga cgcggttcag caacccacgg
     8581 tcgcgtagcg catgaagcat cgcaatacgc ttttcgtccg ggtagtaact gttcttacca
     8641 atggtgtcga actgcacgta cgcgccgaga tcgatcatct tcaaaatgtt gtcgaggttg
     8701 tctttcagat cgcagtgacc aacggtgacg cgcgaaagat caaccccgtg ggcttgtagc
     8761 aacgccagtt gctccagccc catcgtgctg aacgacgtat gcgtggagat cgggcgtccg
     8821 gtctggttat gcgccagcgc agcggcaata aataccttct cttccagcgg cgtaatcttt
     8881 ccttcgctgg tgccgatctc cgcgatgatc ccggctttca gctccgtgcc atcgataccc
     8941 tgttcaattt catcgaccat ctcctgcgcc agttcctgca cgctgcgggt cgccacatgt
     9001 tccgggaaaa acgcgtcctg gtaataaccg gtacaggcca ccacgttgat ccccgtctcg
     9061 cgcattacat caagcataaa ttgcgcattg cgccccatgt aacggttggt catctcaatc
     9121 acattacgca cgccccgggt catcaggtcg ttcatctcct ggcaaatgaa cgcatactga
     9181 tcaaggcggc agtccacgtt gtttttaaag ccggagagat caatatgcag atgctcatgg
     9241 gcgagggtgt aacccgtcgg atcaaaactc atagggtgtc tcctgcaaac cggagggagg
     9301 aaagaaaaga gcgaccattt tgtggcggtg gcgcgcggaa aaattcacac acggtagccc
     9361 ccacatcaga aagcgtggtg cgcacaccga gctgcgtagc gatcatccct tgctgataaa
     9421 ccagcactgg caccacttcg cgggtatggt ggctgtgacc aatggtcgga tcgttgccgt
     9481 gatccgccat cacgaccagg caatcatctg gctgcatcgc ctcaacaagc cgggcaaggt
     9541 tacggtcaac gacctgcaaa cgttcggcat aacgtgcgac gtcttctgca tgaccagcga
     9601 ggtcggtttc ctgaatgttg gtgcaaataa acgccgtcgg atgggtgtta aattcgttga
     9661 gggtgatatc cataatccgc tggctatcca ccaaattttg ccagctcacg ccataaggat
     9721 tgttgacgat atctgccacc ttacccacca gcacggttgg cacgcctgct tcatacagtt
     9781 tttgtggcac ctgcactttt tcatcgacgc catagcccat atgcacgacc tggaaaccgt
     9841 tgtcataagc gccagaacgc ggcgcattga taccaataaa gcgcccttct ttgctttctg
     9901 ctgcatcgag aatgcgttga ctgtcggtta acaggccacc aaatgtaatg acccgaccga
     9961 cctgtacctg ctcacgcacg atacgaccaa ttttgattgc gtcgtcaaaa gagatcacag
    10021 agagattggc ggtaatgtta tagacctggc ctaaatccgc ctcgagatta tcgccaatcg
    10081 caaccgcctg attgacccac agaaattgca gatcatcgcc acggcgctcc acctgccagc
    10141 cagcggaaac taatgcctgc tcaacacggt caatcacatc gcgaaaaggc atccgcagcg
    10201 gcggtaacgg gcgcgtgcct aaaatttcct gatgccccat aaaggtatcg ccaccttcat
    10261 gttgcagctc tgccacgccc caggttgcgg aatctgacgg ctgcatatcg cctggcgcat
    10321 aacccaatgc gttgattagc cccagcttct ccagcgttgg tagctgcaaa tgcggcaact
    10381 ggctcaggat gtgaccacat gtattcgctc ccgcatcttg cggacgcacc agcgtgacat
    10441 ctttcattgc ccctacgcca aagctatcaa tcactaacac cacaaatcgc gccattacgc
    10501 ccccagagag tttccaagac tgtcataacg accgacgatt tccggttcgc cacgatgaat
    10561 accggacacc agcaccacat cactacgagt gacaaaaatc tgcgtgcgaa aacagagcac
    10621 cactgcactg cttaccggaa actcgcctgc cagcggcagg gtgtagtcga tactgctgtc
    10681 atccacagtt ttgagattgg tttcagtaat cttttgattt tctggcgtaa acaccaacgc
    10741 atgttgcgca tgaccacgac gatagtaacc gccgccgtag cagtagctgt cgccacggaa
    10801 atgatgggag atttcactta accagagcat cgcgatacgt tcaggctgat cgccctgctg
    10861 gtttgccgga atagtgcccg tcagcgcatg accgggttcg gcatgagtca caccgtattg
    10921 cgccagtaat ggcagcgaag tgcagctggt cgctgaaggc gcgttcagtt gctcaagtgc
    10981 aataccagat ttcgccagtt gatcccgtgc ctgtatcagc gtgtgaagat tcggtgtcgg
    11041 caaaactttt ccgacagcct catcccaaag caggcaaggg aaatgggtaa gtccggctaa
    11101 atgcagccct ggcagattct ggatttcggc gacaatctcc ggcaacacct tgagggcaaa
    11161 accgctctcc tggcccggat aaagaaaatc atcgtcgcta taaactttaa gcagcacaga
    11221 ctggattcgc ccggccttca ccgccgccgc agaaacttcc cgcgctttgt cgagagtaaa
    11281 cacggtgatg acgtcggtgc cctgttcaac ggcgtcagca acctgatgac aagggatttg
    11341 taccagatgc ccctgatgcg ccacaggcaa accagcgcgg cgcatgactc gcgcctcttt
    11401 gtaatccacc gccacaatgc cgctgtagcc taatgccagc aatttttccg ccagccacgg
    11461 attacgacca aactgcttgg tcatcagata cagttcaatg ccgtaaagcc gcgccgtctc
    11521 aatcagccgc ttaccgtttt ccagtatctg atccacgtcg atcacccagc tgtccggggc
    11581 gatcttgccc tgctgccaca ggcttagtgc ggcggaaatc agcgccgggt tctggcgttt
    11641 caatgcttct acaaacatct tgttgcctcg tttctgttcc ttgaattaaa tattcatttt
    11701 tttgaatatt taggttgtgc gacgtggatt tgtcgggagc ttcaaacctc ttcgttagca
    11761 gccatccata atccataaag gttggccagc aaatagcctt cttcacacgg gttaacttcc
    11821 agtgcgaatt ccttcaacaa cacctgatgc aattgcacaa cggcttgcca gtggctggat
    11881 tgcgccagtt ctgccagcaa ctcgttatcc agcggctcta tttcttcacc acggcgactg
    11941 cgcatcagtg cactcgccat atgtgtcatc gccatcgttc cttgctcact gcgcaccggc
    12001 agatggcact ctgtttccag tacgttgacg acctgcatca tgcctttgca gatgtcctta
    12061 tcaataacgc ctgcctcgca aagcaggttg agtctggttt ccatatcaga tgcctttcgt
    12121 ttaataactc ggttcctgtt ccccactcac tacccgttgt tgatgggcga gcagggcgtg
    12181 tttatctaca acggcacgca gcagttgttg cattgggtaa tcatcgactc gcgtcaggac
    12241 cacggcttcg gtggcctgta aaaatcgcgg atcgtctgtg agcggcgtcg cctctaatcc
    12301 cagcatggtc agttcgtttt ccgccaccac gttccagatc accgcatcga catcgccttt
    12361 aacaatgcgt tgtaaactct cgtgataaga gagatcgact cgttccacat cactaccgcc
    12421 aaaaaaaaca tcggtcatga ttttctgatc cgccgaacgg ttatccagcc ccacgcgctt
    12481 cacgtttgcg gactcgcctt tacggcaaat caactggtgc tcgccaacgt aggtgtgcgg
    12541 ccccaactcc agcgcgaggc ataacccttt ttgcgtgaga taactttccg ccgccagtcg
    12601 ggaaaccacc gccatgtcat acacgccgtt aagtaaacac tccacgcgaa tatccgcgcc
    12661 gcgcatatgc gcatagtaaa aaggaatgcc atcaaactga gctttcagcc cgctcgctaa
    12721 accttcgtac aagcgggtat agggcaaagg catcgcacat accacgttgt tgatatccac
    12781 atgactcagc aatgctttgt tatccatctc gaccagataa ctgccattgc gcccacggcg
    12841 ttcaatccgt atcgctccgc ttgattccag cgttttcaac gcggcctgcg tcaggccaac
    12901 cgatgaacgg cattcagttg ccagctcatc tatggttttc aacctattac cgcatttttc
    12961 acccaacaaa taacgggcca gcgtcgtgat aacgacgcct tcttttttga taaacgttcg
    13021 acgcataata tattttcaat aaaatgaatg tttatatctt cattaaaatg aagataagtg
    13081 gcaactgtga gatgtggaaa cggcgaggcg cttcacattt tttcggatta ttgcaaatga
    13141 ggataaaaaa accgggtttc cccggtttga gagtgatgac gaagtgattt attcgacaat
    13201 gcgagcaaag ggtaattccc actggcgctt gcctgctccc ctcaccctaa ccctctcccc
    13261 aaaggggcga ggggaccgtc cgagcgtttt gatattacgg cttcgccaca aaaccaatcg
    13321 cttcgtatac cgcttttagc gtacgggaag cgtgcgcgct ggctttttcc gcgccatctt
    13381 tcatcacttg ttgcaggaag gcttcatcgt tgcggaaacg gtgatagcgt tcctgcaatt
    13441 cagtcagcat accggaaacg gcatcagcca cttcgccttt cagatgacca tacatcttgc
    13501 cttcgaactg tttttccagt tccgggatgc tctggcccgt taccgcagaa aggatatcca
    13561 gcaggttgga aacgcccgct ttgttctgca catcgtagcg aactaccggc ggctcgtcgg
    13621 agtcagtgac cgcgcgtttg attttcttca ctaccgattt cggatcttcc agcaggccaa
    13681 taacgttatt gcggttatcg tcagacttgg acatcttctt ggtcggctcc agcagcgaca
    13741 ttacgcgcgc gccagatttc ggaataaacg gctccggcac cttaaagatc tcgccataca
    13801 gcgcgttgaa acgctgggca atatcgcggc tcagttcgag gtgctgtttc tggtcttcac
    13861 ccaccggtac cagattagtt tgatacagca ggatgtccgc tgccatcagc accggatagt
    13921 caaacagacc agcgttgatg ttctcggcat aacgcgcaga tttatcttta aactgcgtca
    13981 tgcgactcag ttcgccgaag taggtatagc agttcagtgc ccagcctaac tgcgcatgtt
    14041 ctggtacgtg ggactgaaca aaaatggtgc ttttctcagg atcgatacca caagccagat
    14101 acaaggccag cgtatccagc gtcgctttac gcagcttctg tgcatcctgg cgcacggtga
    14161 tcgcgtgttg gtcaacgata cagtaaatgc aatggtagtc atcctgcatg tttacccact
    14221 gacgcagcgc acccatgtag ttaccaatgg tcaattcacc tgagggctgt gcgccactaa
    14281 aaacgatggg cttagtcatt tttcgattcc tgattttcgc tatgcggaag ccctaatgcg
    14341 ggcagaaggt catttataga ctgataaatt acatcaggct ggctgagatc gatagcctcg
    14401 ccgtagttat atccgtaggt taagccaacg gatgggcaac ctgccgcttt tgccgcctga
    14461 atatcattgc gtgagtcgcc gacaaacagc atctgttgtg gggcaattcc catccgctca
    14521 gccaccagta acagcgggtc cggatgaggt tttttgtttt gcacatcatc gccgccgata
    14581 accacgctaa agtatttggc aatatctaag gcttcgagca gcggcgcgac gaacggcgtc
    14641 ggtttgttgg tgaccaggcc tagcggcagg cctttagcct gcaacgcgcc caacgtatcg
    14701 gcaacgtgcg ggaacaaaaa cgtcccctct tcggcaacct cgccataata gcgatcgaac
    14761 agtttacgca gaatacgtac ctgttcttct gcaggaatgt cgtcatcaac gggcggttta
    14821 cccattgttt tacgcagagt cgcacgttcc tgacgcgccc aggtcaatgc gcgctccatc
    14881 agaacatctg cgccgttacc aatccaggta ataacgcgtt cttcacctgc gacgggtaac
    14941 tccaacgcat acagcgccat atctaccgca gcagcaagac cgggagcact gtccaccagc
    15001 gtaccgtcga gatcaaaagc gacgccgcga atatcttcaa acttactcat gacttacctt
    15061 tgccagttca ctgcgcattt catcaatgac ttttttatag tctggctggt cgaagattgc
    15121 cgaaccggcg acgaacatat ccgcgcccgc cgcagcgatt tcgccaatgt tgttcacctt
    15181 cacgccaccg tccacttcca gacgaatgtc aaagccagac tcgtcgatac ggcgacgtac
    15241 ttcgcgcagt ttatccagtg tttgaggaat gaaagactga ccgccgaaac cagggttgac
    15301 ggacatcagc aggatcacat ccagcttatc catcacgtaa tccagatagc tcagaggtgt
    15361 cgccgggtta aataccagac ccgctttaca gccattttct ttaatcagtt gcagcgtgcg
    15421 gtcaacatgc tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag cggcagcgaa
    15481 atcaggaaca atgcgatcga cgggtttcac catcagatgt acgtcgatag gggcggtaat
    15541 gccatagtta cgcaaggatt tcagcaccat tggcccaatc gtcagattgg gaacatagtg
    15601 gttatccatg acgtcaaaat gcacgacatc agcgccagct gccagggctt ttgcggtatc
    15661 ttcacccagg cgggcaaaat cagccgacag aattgagggg gcaatcaaat actgtttcat
    15721 ccgcttctcc ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt gtacaaagcc
    15781 agcagttcgt ccaccttttt acgtgtaccg ccgttgctgc ttatactgcg tcgaactttg
    15841 acgacatgca attttgcgcg ctgataccac tcacgcgtta acatcgtatc gtgattggag
    15901 atcagcacag gaatatggcg atcaaccaga ccttcggcga tctccgccag atgcgcttgt
    15961 tgttcaagcg taaaactgtt tgtgtgatac gccgtaaagt tggcggtcgc agacagcggt
    16021 gcataaggcg gatcgcaata gacgacggat gcatcatctg cgcgcgccat gctatcggcg
    16081 taagactcac aatagaaaaa ggcattctgc gctttttcag cgaagtgata caactctgct
    16141 tccgggaaat agggtttttt gtagcggcca aacggcacgt taaactcacc gcgcagattg
    16201 taacgacaca ggccgttgta accgtagcgg ttcaaatata aaaacagtac cgcccgacgg
    16261 aacggatcct ggcttttgtt gaactcttcg cggaactgat agtaaacctc ggcgcaattt
    16321 gtttcgggaa caaacagctc gcgtgcggcc tgtacgtact catcagtacg catcttcaca
    16381 atgttataga gactgatcag gtcgctattg atatcggcaa ggatataacg agaaaagtcg
    16441 gtgttgagaa acaccgaccc ggcacctaca aaaggctcaa ccagacattc gcccttgggc
    16501 aaatgccgtt taatatcatc aagcagggga tacttgcccc ctgcccactt caaaaaagcg
    16561 cgatttttct tcatgctgac taactaatta caccttctcc ggctgtggag aaagctccga
    16621 cagcatccag cgcttcaggc aactcccgca acgataaacc attgcgggag ataaccttga
    16681 ttacttcaga tcggcctgta cctgacgcag cggtttcgcc cacgggtttt tggcctgaac
    16741 atctgctggc aatgtagata ccgctttttt cgcctcttct ttcgaagcgt acacgccaga
    16801 aaccaggaca taccacggct gaccattacg cgtcgtttca tagacaacgt agtttttcag
    16861 attctctttc ttcgcccaac cgttcaggtt gtcgtagtta gaggaactgc tcagctgcag
    16921 agtgtaatgg ctggacggtg ccgatttcaa cgaaccaaca ttacctgcgg tcttccctcc
    16981 agcggctggt gttgccgtgg tttgcgccgg ggatgccgtc tgtactggag ccgtagtcgc
    17041 cgtctctttt ggtgctggcg tagaagtcgc agccggtgcc ttcgtgctcg ccacaggggc
    17101 agctggttca gtacgcttcg gcgtctgtgc taccggcttc ggctccgttt tcacggttgc
    17161 ttgcgatttt ttcggttcaa tcactgcctg ctggcgagct ggacgcgtag tggccggacg
    17221 ttcagcggtt tgcgttttcg ccgtttcacg cgatgcattg ccattgcgaa caggcgcgac
    17281 agtcgcgggt tcggtcggca atgtggaatt gaccgccaca ttgttcaact gttgctgatt
    17341 ttgtggctgg gtcagcgcat tgttcaggtc accctgcact tcaacacgtt gttgaccatc
    17401 cgttgccacc ggggtttgcc cttgagtcgg cgtagaagag atcggtggca gagaaacatc
    17461 ctgctgagta ttttccgcag acgtggttcc cggcgctggc tgcacgccat tcgcctgatc
    17521 ggtcgcattg ccagcaagat caatactctt ctcgccagac gcggtttgat cgctggaagt
    17581 ggtcgagggg gcttttagcg cagaaccgat accgatgatc aacagcagta gaaccagaat
    17641 gccgacgccc atcatcatat actgacgaga agcgggttta ctggctgctt ttttgcgctt
    17701 acgcggacga cgctctacgc gctcttcatc cacggtttca tcttcggatt cgtcaatttc
    17761 ttcttcgatt tccggttcct cattgcgctc tttttgcgca cgagtcggac ggcgatcgtc
    17821 agtgtcatca agttcaatat catcaaaatt gatctgcggt tcgccacgtt cagtacgctc
    17881 agaacgttca gaagattgac gagaacgacc agtacgacga tcgctgggat cgggtttcag
    17941 ctcgtcttct ggtttgaatt catccattta acaccccact aaaaggttaa tgcttaccac
    18001 gttgcaatta acctgaagct aatagaccgc ttgataagcg gcctgacctt tcttgttgtt
    18061 acgctgattg acaatcggca atggcgttaa gaacaagctc gtgcgaaacg ccgctgcgaa
    18121 cttcactctt accaattgcc aacggaagaa ttaagcgcat ctctcccgca aggactttct
    18181 tgtcacgcag catatgcggt aaatacgcct gcgcggacat ttcgcgcggc ccattgaccg
    18241 gtaacccagc ccgcgtgagc agggttataa tacgctgcgt ttcggcagaa ctaaactgcc
    18301 cgagacgttc cgacgtccgc gccgccatca ccatacccgc agcgaccgct tcaccatgta
    18361 accaattgcc ataccccatt tcagcttcaa tggcatgacc aaaggtgtgt cccagattca
    18421 gtaaagcacg taacccggtt tcgcgctcgt cggcggcgac aacttctgcc ttcagttcac
    18481 aacaacggcg aatacagtac gccattgccg gaccgtccag acgcaacaac gcatccagat
    18541 tctcttccag ccagttaaaa aacgcaccat caagaataat gccgtatttg atgacttctg
    18601 ccagccccga cgctaactca cgcgggggaa gcgttttcag acagtcgaga tccaccacca
    18661 ctgaagcagg ctggtagaac gcgccaatca tgtttttacc gaggggatgg ttgaccgcag
    18721 ttttgccgcc aacggaggaa tcgacctgcg acagtaacgt cgtcgggact tgaatgaaac
    18781 gaacaccgcg ctgataactc gccgccgcga agccggtcag atcgcccact acgccgccgc
    18841 caagcgccac cagcgtagta tcgcgaccat gcggcttttg taacaacgcc gtaaagacgg
    18901 tatcgagtac agccaggctt ttatactgct cgccgtcagg gaggataacg ctatcgacgt
    18961 taacacccgc ctgttcaagt acgccgcgga ccttatcgag atacagagga gccagggttt
    19021 cgttggtgac caacatgacc tgctcgcccg atttcagcgg taagaatgaa gctggttcat
    19081 taaacaaacc agatgcgatg gtaattgggt aactacgttc cccgagagta acgacaatcc
    19141 tctccataac gcgacatcca ccttaattac tgtacccgca gacgagtgta tataaagcca
    19201 gaattagttg ctttccagca tgtgaataat ctggtttgca accactttag cgctttgatc
    19261 atcagtacga atggtcacgt cggcaatctc ttcatacagc ggattgcgtt cattggccaa
    19321 cgcttccaga acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt ttttatcacg
    19381 ctgcgtgcgt gcaagttgct tttcgatggt cgtttcaaga taaacgacaa cgccacgagc
    19441 ggaaagacgg ttacgcgttt cacgggattt cacagagccg ccgccagtag ccagcacaat
    19501 accctgtttc tcggtcaact cattgatgac cttttcttcg cgatcgcgga agccttcttc
    19561 gccttctaaa tcgaaaaccc agcccacatc agctccggtt cgtttctcaa tctcttgatc
    19621 ggaatcgtaa aattccatat tgagttgttg agctaactgg cgcccaatag tgctttttcc
    19681 ggcacccata ggcccaacca gaaagatatt gcgtttctct gccatttttt cggtactact
    19741 aagactattc gttaatgata aacccgcttc gctcagagag cgccgcagga catgaactga
    19801 aacctcataa gatattgcga gagtcagact gaaaattatc tcaatactcc agcgggtttg
    19861 gcaactgaat aaatcaccca tccctctgca tatctggtcg ctgcaagcgc gctgccttgc
    19921 taccaccgct ctggcgataa atcaccgggt aagattagcg taaaaaagac agcaaaatgc
    19981 cgcctgaatg ataaatcatc atcatggaac gcaggcagcg aagagcacca actctcaaat
    20041 cggtactcct tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac gttcagcata
    20101 aaaacattac cgcttttacg gctgtttact cactggaaac cagtcgtggc gtgataaaca
    20161 ccactaactc gcgtcgttca tcttcttttc cgtcatgacg aaataattgc ccgaaccagg
    20221 gaatgtcgcc aagcaacggt acgctatcct gacccgattt atttttacgg gtaaaaatgc
    20281 cgcccagcgc caacgtttct ccgcttttga cctcgacctg cgtttcgatc tcctgcttat
    20341 caatcgccag cacttcgcca tcggcctgct gtagcacctg ccccggaacg ttctggctga
    20401 tgtgtaattt cagccggatg cgaccttttt gtaacaccgt gggcgtgacc tccatcccca
    20461 ggacggcctc tttaaattcc accgacgtcg cgccactttc cccgctggaa acctgatatg
    20521 gaatttcgct cccctgttta atgctggcag gctgaagatg tgaggccagc agacgcggac
    20581 tggcgataat atccagctgc tgtttttgtt cgagcgcgga aagctcaaga tccagcaaac
    20641 gtccgttgat gcgcccaatg ttaaaaccga catgcgttgt cgccgtcgct acggagaggt
    20701 cgctaccaag cgtggtgact tgcccaacgc caccagcgtg ttgcgcatcg gccagcgtcc
    20761 atttcacgcc taactcacgc aaactttttt cattaatggt gacaatatgc gccgacagct
    20821 caacctgccc gaccggcaga tccatttgcg ctacccactg ttcaagcgcg cttaacgccg
    20881 ttttgttatc tcgtagcaaa aggcgattgg tgcgtttatc gacggtcata ctccctttgg
    20941 cactcagtag cttctccccc gctttcgcca gttctcccgc gtcggcgtat tgcagggtta
    21001 tactgcgatt ttccagcggc agatttgcct gcgcccgcgc ctgctccgcc tcctggcggg
    21061 cgatattgtt attctgccag gcaatggaat gcactgagag aatgttgcct tcctgccgcg
    21121 ttatcagtcc ggcgcttttc actacagttt gtagtgcctg cttccaggga acatctgtta
    21181 gatgtaacga caccgtaccg ctgacgtctg gcgacacgac caggttcaac ttctcctgtt
    21241 cagccagcgc ctgcaacacc tgagctaccg gaacgtcatc caccatcagc gtcacttttt
    21301 gcggctttgc cgcctgtacg ccgggtatca gcatcaacag tagtgcggct atccattgct
    21361 tcatttgtat ctccttgccg ttgccacaac cattgtggcg gttcgcagtt tgtcccggta
    21421 cccagcgtta gtacgtctgg cgtcagctgt aaaattgtcc agccgttttc cagcacatcg
    21481 ttttgctgca cccgtcgcca tttcttttgc ccgtctttta ttacaccgat gatgcgctcg
    21541 cctcgcccta ccatcccctg atagcgccac tggctaagtt cgctaatccg gcatagatct
    21601 tccggcggtt taaaagggtc acgcataccg gttaaaaggc acaatgcaat acctgccaac
    21661 aaccagcgtt taaccctcat gcggcgtctc cagttgtagc gtgaacaaaa gatcatcacc
    21721 ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc tctgccagcc gtgtaaatgc
    21781 cgacggcact gcttcccaca gcgttttcaa caccaactcg cctccctgcg cggatggatg
    21841 ccaggaaacc agttgcgcgc cggataactg aaaatccagt ggcgaaaagg gcagcgtttt
    21901 ttcctcgctg aagggagtgg tgtctaccag gcgatacagt gcggcccact gctggtgatg
    21961 acttgcccgt agtcgaatta atgcgtcgcg ctcttcatgg tgtgtcgatg acagaaaaat
    22021 gagcgtaact aacatcagca accagaatgc ccagcaaagc tggcggaggc ggggtgatgt
    22081 ggcgaaccac cagtcaaaga acatgttcat cgctaacctt ccttgttaac tgatactcaa
    22141 attgccagcg tccctgcgca tcctgctgcg tggctccccg ctgattgaga tgaaaagatg
    22201 catcctggcg gagtgacgtt tctagtgcgt ttaacgcggt aatgcttgtt gtcagcccct
    22261 tgatctccag cgttccctgc tgccagctta tcgttgtcag ccaggcgtgc tcgggtaaaa
    22321 gcgccgccag tgcttccagc gcagattgcc agtcgcgggt aaattgtcgc tggcgctgcc
    22381 gttgcgaacg ctgttcgcgt aactgctgtt gctccagcaa acgtggcttc gttatctgta
    22441 agctgcgggc gagttgttgt tccgcctgaa gcaaaactgc gtctatgcgc gcttcggcgc
    22501 tgcctgtcag acgcagtatt agcgttatcc cgacggccag cagcagaggc gcaacgaaca
    22561 tcagcaacca gaaacgcaga aaagcggtcc ggcgttgctg tcgccagggc aaaaaattaa
    22621 ttggcgggtt catcagtacg ccttcccaag cgccagcccc agcgcgatgg caaagtctcc
    22681 acctggcggc ggtagcggcg gctgacgaac agaaacggcc tcccagggat caaatccgcc
    22741 ttcgccacat atcgcgacgc tttcgggatc aacggataac gctgccgcca gctccttcgc
    22801 gctagtcatc cccaccgcca gtttgcgccc ccagctatag cgcgtcgccc acagccactg
    22861 ttcgttatca cgccaggcca gacattgctg atgagaaggt aaaaaaggca ggaatcgctg
    22921 taatgcactg gcatccgggg tgatcgcact aacatgaaca cgcaaccttt ctgccagagt
    22981 aagcagcgtt gccagctctt tgctttgcgc ggcagtcacg ttataggcgg gactgagtga
    23041 gtcttcgctg taatcgaagc gcagggagtc cggatccata tccagctcgc gggccatcgt
    23101 ccctgacagc caggccgttt gctcccgctc accaagggac atcgacgggc gcggaaatga
    23161 ccgctgtaat gtgcgactgg cgggaaacgc caacataatg tgatgacgct gcggcagttc
    23221 gcgactccac ggtaacaacg ttttagccag ctgctgcgca tcaacaatcc gcccatcttt
    23281 gataatgtcg ttctccagcg gcaaccgcca ccagcgttgc aaaaagcatt cttttgcgcc
    23341 ccgcacgatc gcaaccgcta ccgcttcttg ctgttgtaaa tgcaaaccaa tttgccagat
    23401 cttaaatgcc attgtgatga tctccttatc acccgtcact ctgacgggta tatcaatgcg
    23461 tctggcttgc ctttatacta ccgcgcgttt gtttataaac tgcccaaatg aaactaaatg
    23521 ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct tgcagtctgt tgcattctgc
    23581 tgggagcagg ctcgatttat ggcctatacc gctacatcga gccacaactg ccggatgtgg
    23641 cgacattaaa agatgttcgc ctgcaaattc cgatgcagat ttacagcgcc gatggcgagc
    23701 tgattgctca atacggtgag aaacgtcgta ttccggttac gttggatcaa atcccaccgg
    23761 agatggtgaa agcctttatc gcgacagaag acagccgctt ctacgagcat cacggcgttg
    23821 acccggtggg gatcttccgt gcagcaagcg tggcgctgtt ctccggtcac gcgtcacaag
    23881 gggcaagtac cattacccag cagctggcga gaaacttctt cctcagtcca gaacgcacgc
    23941 tgatgcgtaa gattaaggaa gtcttcctcg cgattcgcat tgaacagctg ctgacgaaag
    24001 acgagatcct cgagctttat ctgaacaaga tttaccttgg ttaccgcgcc tatggtgtcg
    24061 gtgctgcggc acaagtctat ttcggaaaaa cggtcgacca actgacgctg aacgaaatgg
    24121 cggtgatagc cgggctgccg aaagcgcctt ccaccttcaa cccgctctac tcgatggatc
    24181 gtgccgtcgc gcggcgtaac gtcgtgctgt cgcggatgct ggatgaaggg tatatcaccc
    24241 aacaacagtt cgatcagaca cgcactgagg cgattaacgc taactatcac gcgccggaga
    24301 ttgctttctc tgcgccgtac ctgagcgaaa tggtgcgcca ggagatgtat aaccgttatg
    24361 gcgaaagtgc ctatgaagac ggttatcgca tttacaccac catcacccgc aaagtgcagc
    24421 aggccgcgca gcaggcggta cgtaataacg tgctggacta cgacatgcgc cacggctatc
    24481 gcggcccggc aaatgtgctg tggaaagtgg gcgagtcggc gtgggataac aacaagatta
    24541 ccgatacgct gaaggcgctg ccgacctatg gtccgctgct gcctgccgca gtcaccagcg
    24601 ccaatcctca ggaagcgacg gcaatgctgg cggacgggtc gactgtcgca ttgagtatgg
    24661 acggcgttcg ctgggcgcgt ccttaccgtt cggatactca gcagggaccg acgccgcgta
    24721 aagtgaccga tgttctgcaa acgggacagc aaatctgggt tcgtcaggtc ggcgatgcat
    24781 ggtggctggc acaagtgccg gaagtgaact cggcgctggt gtcgatcaat ccgcaaaatg
    24841 gtgccgttat ggcgctggtc ggtggctttg atttcaatca gagcaagttt aaccgcgcca
    24901 cccaggcact gcgtcaggtg ggttccaaca tcaaaccgtt cctctacacc gcggcgatgg
    24961 ataaaggtct gacgctggca agtatgttga acgatgtgcc aatttcccgc tgggatgcag
    25021 gtgccggttc tgactggcag ccgaagaact cgccgccaca atatgccggt ccaattcgct
    25081 tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt acgcgcaatg cgggcgatgg
    25141 gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt cccggcacaa aacattgtcc
    25201 acaccgaatc gctggcgctg ggttcagcgt ccttcacccc aatgcaggtg gcgcgcggct
    25261 acgcggtcat ggcgaacggc ggcttcctgg tggacccgtg gtttatcagc aaaattgaaa
    25321 acgatcaggg cggcgtgatt ttcgaagcga aaccgaaagt agcctgcccg gaatgcgata
    25381 ttccggtgat ttacggtgat acgcagaaat cgaacgtgct ggaaaataac gatgttgaag
    25441 atgtcgctat ctcccgcgag cagcagaatg tttctgtacc aatgccgcag ctggagcagg
    25501 caaatcaggc gttagtggcg aagactggcg cgcaggagta cgcaccgcac gtcatcaaca
    25561 ctccgctggc attcctgatt aagagtgctt tgaacaccaa tatctttggt gagccaggct
    25621 ggcagggtac tggctggcgt gcaggtcgtg atttgcagcg tcgcgatatc ggcgggaaaa
    25681 ccgggaccac taacagttcg aaagatgcgt ggttctcggg ttacggtccg ggcgttgtga
    25741 cctcggtctg gattggcttt gatgatcacc gtcgtaatct cggtcataca acggcttccg
    25801 gagcgattaa agatcagatc tcaggttacg aaggcggtgc caagagtgcc cagcctgcat
    25861 gggacgctta tatgaaagcc gttcttgaag gtgtgccgga gcagccgctg acgccgccac
    25921 cgggtattgt gacggtgaat atcgatcgca gcaccgggca gttagctaat ggtggcaaca
    25981 gccgcgaaga gtatttcatc gaaggtacgc agccgacaca acaggcagtg cacgaggtgg
    26041 gaacgaccat tatcgataat ggcgaggcac aggaattgtt ctgattaaaa aggcgcttcg
    26101 gcgccttttc agtttgctga caaagtgcac ttgtttatgc cggatacgga tgaacgcgtt
    26161 atccggctaa caaaatcgtg aaaaatcaat atattgcagg aaccccataa gcctgataag
    26221 cgttgtgcat caggcaaact tcacgcattt acactcgccc ctgccctttc aaccattcgc
    26281 gcacgaggaa cagcgcgctg acattacgcg cttcgttgaa gtcagggtct tccagcaaat
    26341 ccatcatatg cgccagcggc cagcgcacct gtggtagcgg ctctggctca tcgccttcca
    26401 gcgattccgg gtagagatct tgcgctacca cgatattcat tttgctggaa aaataagacg
    26461 gtgccatgct gagcttcttc aaaaaagtca gatcgttcgc accaaatcca acctcttctt
    26521 ttagctcgcg gttagcggct tcgtagacgc tttcacccgg atcaattaat cctttcgaaa
    26581 aacctaattc gtaggattca gttcccactg cgtattcgcg gatcaggatc aggtgatcgt
    26641 ccacaatcgg cacaatcatc actgcttccc ggttggttgg acgcattcgt tcataaacac
    26701 gccgcacgcc attgctgaac tctagatcca cgctctcgac ggtaaacagt cgggaacggg
    26761 ctacagtttc aacattcaga atggtgggtt tttgtaatga tttgctcatc gtgggatcta
    26821 tgctgtgaaa tcagcggtta ttgtgcgata tcggacacgc tttcggcaat gtgaattgca
    26881 tgttatttac atttatgtaa cttaataaat aatcgtcctc aaatcaaatt aaaagtcaat
    26941 aggttgaaat aactccagga atttgctgat attccgcctt cagagggttt gctatgatca
    27001 gcggttactg tgatgtgctt aatgatgctc aagttaaact ccacgcttgc cgatagccaa
    27061 ccgcagaatc atgtattgtg tccggtgcga ctgaccacgc ctgacagact aagtaagatg
    27121 gggaaagcat gagcaccatt gtgatttttt tagctgcttt gctggcctgc tcactacttg
    27181 cgggatggct gataaaagtg cgatccagac ggcgtcagct gccctggacc aacgccttcg
    27241 cggatgcgca aacgcgtaaa ctcacacctg aagaacgtag cgccgttgaa aattatcttg
    27301 agagcctgac gcaggtatta caggtgcctg gcccaacggg agccagcgcg gcaccgatct
    27361 ctctggcgct gaatgccgaa agcaacaacg tcatgatgct gacacacgct atcacgcgtt
    27421 acggcatctc taccgacgat ccgaataaat ggcgttacta cctcgattcg gtagaagtcc
    27481 acctgccccc tttctgggaa caatacatca acgatgagaa taccgttgaa ctgatttata
    27541 ccgattcgct gccgctggtt atttcactca acggtcatac gctgcaggag tacatgcagg
    27601 aaacgcgcgg ctatgccttg caacctgttc cgtccacgca ggcgtcgatt cgcggggaag
    27661 agagtgagca aatcgagcta ctcaatattc gcaaagaaac gcatgaagaa tatgcgctga
    27721 gtcgtccgcg cgggctgcgt gaagcgttgc tgatcgtcgc ctccttcctg atgttctttt
    27781 tctgcctgat taccccggat gtatttgttc cgtggctggc aggcggcgca ttactgctgc
    27841 tgggcgcagg tctgtggggg ctattcgcgc ccccggcaaa atcctccctg cgagaaattc
    27901 attgtctgcg cggtacaccg cgtcgttggg gattgtttgg cgaaaacgat caggaacaga
    27961 tcaacaatat ttcactcggt attatcgacc tggtctatcc cgcacactgg cagccataca
    28021 ttgctcagga tctcggtcaa caaaccgata ttgatatcta tctcgaccgc cacgtagtgc
    28081 gtcaggggcg atatctttcg ctgcatgatg aagtaaaaaa ctttccgtta cagcactggc
    28141 tgcgcagtac gattatcgct gcgggctcgc tgctggtgct gtttatgctg ttattctgga
    28201 tcccgctgga tatgccgctg aaattcactc tctcatggat gaaaggcgcg cagaccattg
    28261 aagccaccag cgtaaaacaa ctggctgacg ctggcgtgcg ggtaggcgat acattgcgta
    28321 ttagcggtac gggaatgtgt aatattcgaa cttccggaac ctggagcgcg aaaaccaatt
    28381 cacctttttt accgtttgac tgctcgcaga tcatctggaa cgacgcccgc tcattgccat
    28441 taccagaatc tgaactggtc aacaaagcga cggcattgac tgaagcagtt aatcgccagc
    28501 tgcaccctaa accggaagat gaatctcgcg tcagtgcctc attacgttca gcaattcaaa
    28561 aatccggcat ggtattgctt gatgattttg gcgacattgt actgaagaca gcggatttat
    28621 gttctgccaa agatgactgt gtgcgactga aaaatgcgct ggtcaatctc ggcaacagta
    28681 aagactggga cgcgctggta aaacgcgcca acgccgggaa gctcgatggc gtgaatgtgt
    28741 tattacgccc ggtgagtgcg gaatcgctgg ataacctggt ggcaacctcc accgcgccgt
    28801 ttatcacgca tgaaacggcg cgagcggcac aatcactaaa cagtccggcc cccggcggat
    28861 tcctgattgt cagcgacgaa ggcagcgatt ttgttgatca gccctggcct tcggcatcac
    28921 tttacgacta cccgccgcaa gaacagtgga acgctttcca gaaactggca caaatgctga
    28981 tgcatacgcc gtttaacgcc gaaggtatcg tcacaaaaat cttcactgac gccaatggta
    29041 cgcagcatat tggccttcat ccgatcccgg atcgttccgg cctgtggcgc tatctcagca
    29101 ccacattgct gctactgacg atgctgggta gcgccattta caatggcgta caggcctggc
    29161 gtcgttacca gcgtcatcgc actcgcatga tggagattca ggcctattat gaaagctgcc
    29221 tgaacccgca actgatcacc ccttcagaaa gccttatcga ataacacgtt tgcgcggcag
    29281 gttatgctac cctgtcgcgc aaattgcttc actctggaga tttccctcat gcatatcaac
    29341 attgcctggc aggacgtaga taccgttctg ctggatatgg acggcacgtt gctcgacctc
    29401 gccttcgata actatttctg gcaaaagctg gtgcctgaaa catggggcgc gaaaaacggg
    29461 gttacgccac aggaagcgat ggaatatatg cgccagcaat atcacgacgt acagcatacg
    29521 ctaaactggt actgtcttga ttactggagt gagcaactgg gtctggatat ctgtgcgatg
    29581 accaccgaga tgggaccgcg tgccgtactg cgtgaagata ccattccgtt tcttgaggca
    29641 ctgaaagcca gcggtaagca gcgaattttg ctcaccaatg cgcatccgca caacctggcg
    29701 gtaaaacttg agcataccgg tctggacgca caccttgatt tattactttc cacccacaca
    29761 tttggttatc cgaaagagga tcagcggtta tggcatgcgg tggccgaagc tacgggtctg
    29821 aaagctgaaa gaacgctgtt tattgatgac agcgaagcga ttctcgatgc tgccgcgcaa
    29881 tttggtattc gttactgcct cggcgtgact aatcctgatt ccgggattgc cgagaaacag
    29941 tatcaacgcc atccgtcact gaatgactac cgccgcctga tcccctcgct aatgtgaagg
    30001 agacgccatg aaagagaaac ctgctgttga ggttcgactg gataaatggc tatgggctgc
    30061 ccgtttttat aaaacccgcg cgctggcccg tgaaatgatt gaaggcggta aggtgcatta
    30121 caacgggcag cgcagcaagc cgagcaaaat cgtcgagctg aatgccacgc tcactctgcg
    30181 ccagggaaat gacgaacgca cggtgattgt aaaggcgatt actgaacagc gtcgccccgc
    30241 cagcgaggca gccttgctgt atgaagagac tgcggaaagt gtagagaaac gcgaaaaaat
    30301 ggcgctggca cgtaaactta atgccttaac catgccgcac ccggaccgac gcccggacaa
    30361 aaaagagcgc cgcgacctgt tacgatttaa acacggcgac agtgaataac tgtcacctgc
    30421 aagagagatg attatgccgc aacatgacca attacatcgc tatctgtttg aaaactttgc
    30481 cgttcgcggc gaactggtaa ccgtttcgga aaccctgcaa cagatccttg agaaccacga
    30541 ttatccgcag cccgttaaaa acgtgctggc agaactgctg gttgcgacca gcctgttaac
    30601 cgctacgctg aagtttgatg gtgatatcac cgtacagctg cagggcgacg gtccgatgaa
    30661 tctggcggtt attaacggta acaataacca gcagatgcgc ggtgtggcgc gcgtgcaggg
    30721 cgaaattcca gaaaatgccg acctgaaaac gctggtcggc aatggttacg tggtgatcac
    30781 cattaccccg agcgaaggcg aacgctatca gggcgtagtt ggtctggaag gtgataccct
    30841 ggcggcctgc ctggaagatt actttatgcg ttctgaacag ctgccgacgc gcctgtttat
    30901 tcgcaccggc gacgtagacg gcaaaccggc tgcaggcggt atgttgttgc aggtaatgcc
    30961 tgcgcaaaat gcccagcagg acgactttga ccacctggcg acgctaaccg aaaccatcaa
    31021 aaccgaagaa ctgctgacct taccggcaaa cgaagtgttg tggcgtttgt atcacgaaga
    31081 agaggtgacg gtttacgatc cgcaggatgt ggagttcaaa tgcacctgct cgcgtgaacg
    31141 ttgcgccgat gcgctgaaaa cgctgcctga tgaagaagtt gatagcatcc tggcggaaga
    31201 tggcgaaatt gacatgcatt gtgattactg cggtaaccac tatctgttca atgcgatgga
    31261 tattgctgaa atccgcaaca acgcgtctcc ggcagatccg caagttcatt aatgagtatg
    31321 tccggcagag agggctctgc cggattttac gactaataca cctgatgcgc tttgtcactc
    31381 attcagattc acattcagcc tttcaccatc cactacaaga tcgcgccacg ctttaggctt
    31441 cgtccccagt tttccatcct tcgcagctgt gagtaatttt tctttcgtta tttcgttcgg
    31501 aagtaaactc atatcaagcg cgtcccattc ttttctgtct gagtcaaagc catagacaat
    31561 gactctgtca tcattaaaag caaacaggat ccgctccgcc tgaccatcac tattcagatc
    31621 ttgctcgacc aggacgcagg cgtctttttc aatacaggtc atcacgttat agcgatcctg
    31681 gattaaggcc gaccagaatg tcgcatccgg tttaacagaa ccaggggcaa ttaacacatt
    31741 atcggctaat actttttccg atacctgttg ctgaagatgt tgctctccat ctaacgccat
    31801 cagcagatcc cgcgcgcgtt tcgggtcttt cataaacccg gcatcgcttt tcagcgactc
    31861 aagcgcagca cgtccatagc gaccgctctg ctcgagcatg tagatagtta cctggtctga
    31921 cgtgtttttg ccgctctgat aacgcgccat atggctgttc acgctaatgc gcatactgtc
    31981 cagcaccggc gaattaagaa gcaccagtat caccaacacc aataacgaaa ccgcaaggtt
    32041 cactttgccc tgaagaacaa ggggattttg cccattacgc cagacgatac tgacaaaata
    32101 ccccagcgac cacaccagta acaccagcac cgccagcgcg ccctgcaagc ggtcgacagt
    32161 ccagccgtac tgagcgaccc gcagccataa cgcccaggcg gcgacgaaca catacagcgg
    32221 agcaaccagc aaagcggttt taatcaggca acgcaacggc cccgtccagg gaagtgacgc
    32281 tttttgcgga tcgcggacaa tagccattaa gatcaattgc aaaaaggcca gcgttaacag
    32341 caacccggcg gcggagatgt ggcgagaaat cgcgctcagg cccgtaaacg gcagggtgat
    32401 gataaacatc agggttagca atgatactaa cggcagcaac cccgtggcga ttagcgtgaa
    32461 caacttttga atagagtcga ttaaacgtga ctgtgttcgc gcgaggatca ccgccagcgc
    32521 ggtaaccaga cctaacgtga gataaataaa ccagtcggtt gcaaaaaaga gcgtattgaa
    32581 aaacgtgatg ccaacaagtt taaacaactc actccacagt aaaagcacca gccacgtcaa
    32641 gccattggcg agaaaaatca ccagtaatat aagtacgtta tgccatactg actggtaaaa
    32701 atagcgatag cggctactgt cattgcggat gcgaaggctt tgttgtatcc acggtagcaa
    32761 caacattgcc atcagcagca gatagcagcc aaaatcccaa agcgcctttt cagctctcca
    32821 ggggttcatg ccatcagttt gccacttcaa ccagccgctc atccccagcg tggcaataaa
    32881 caccagcgcc agccatcccc aaaggcgctt ctgtttaaaa gagatcacgg agaagagtaa
    32941 aacggatgaa aacgcgacgg tcgcaggcac gccataaaca atccaactgt gattttttcc
    33001 ggaaagccag acgatcagca ggtagcaaac cagaccctga agtaatccgg tagcaatcat
    33061 cccccagcgt gttgcgggtg aaagttcaac gttatccatg ttggttatcc agaatcaaaa
    33121 ggtgggttaa ttatcgcatc cgggcagtag tattttgctt ttttcagaaa ataatcaaaa
    33181 aaagttagcg tggtgaatcg atactttacc ggttgaattt gcatcaattt cattcaggaa
    33241 tgcgattcca ctcacaatat tcccgccata taaaccaaga tttaaccttt tgagaacatt
    33301 ttccacacct aaaatgctat ttctgcgata atagcaaccg tttcgtgaca ggaatcacgg
    33361 agttttttgt caaatatgaa tttctccaga tacgtaaatc tatgagcctt gtcgcggtta
    33421 acacccccaa aaagacttta ctattcaggc aatacatatt ggctaaggag cagtgaaatg
    33481 cgcgttaaca atggtttgac cccgcaagaa ctcgaggctt atggtatcag tgacgtacat
    33541 gatatcgttt acaacccaag ctacgacctg ctgtatcagg aagagctcga tccgagcctg
    33601 acaggttatg agcgcggggt gttaactaat ctgggtgccg ttgccgtcga taccgggatc
    33661 ttcaccggtc gttcaccaaa agataagtat atcgtccgtg acgataccac tcgcgatact
    33721 ttctggtggg cagacaaagg caaaggtaag aacgataaca aacctctctc tccggaaacc
    33781 tggcaacatc tgaaaggcct ggtgaccaaa cagctttccg gcaaacgtct gttcgttgtc
    33841 gacgctttct gtggtgcgaa cccggatact cgtctttccg tccgtttcat caccgaagtg
    33901 gcctggcagg cgcattttgt caaaaacatg tttattcgcc cgagcgatga agagctggca
    33961 ggtttcaaac cagactttat cgttatgaac ggcgcgaagt gcactaaccc gcagtggaaa
    34021 gaacagggtc tgaactccga aaacttcgtg gcgtttaacc tgaccgagcg catgcagctg
    34081 attggcggca cctggtacgg cggcgaaatg aagaaaggga tgttctcgat gatgaactac
    34141 ctgctgccgc tgaaaggtat cgcttctatg cactgctccg ccaacgttgg cgagaaaggc
    34201 gatgttgcgg tgttcttcgg cctttccggc accggtaaaa ccaccctttc caccgacccg
    34261 aaacgtcgcc tgattggcga tgacgaacac ggctgggacg atgacggcgt gtttaacttc
    34321 gaaggcggct gctacgcaaa aactatcaag ctgtcgaaag aagcggaacc tgaaatctac
    34381 aacgctatcc gtcgtgatgc gttgctggaa aacgtcaccg tgcgtgaaga tggcactatc
    34441 gactttgatg atggttcaaa aaccgagaac acccgcgttt cttatccgat ctatcacatc
    34501 gataacatcg ttaagccggt ttcaaaagcg ggtcacgcaa cgaaagttat cttcctgacc
    34561 gctgatgcct ttggtgtgct gccgccggtt tctcgtctga ctgccgatca aacccagtac
    34621 cacttcctct ctggcttcac cgccaaactg gccggtactg agcgtggcat caccgaaccg
    34681 acgccaacct tctccgcttg cttcggcgcg gcgttcctgt cgctgcaccc gactcagtac
    34741 gcagaagtgc tggttaaacg tatgcaagcg gcaggtgcgc aggcgtatct ggttaacact
    34801 ggctggaacg gcactggcaa acgtatctcg attaaagata cccgcgctat tatcgacgcc
    34861 atcctcaacg gttcgctgga taacgcagaa actttcactc tgccgatgtt taacctggcg
    34921 atcccaaccg aactgccggg cgtagacacg aagattctcg atccgcgtaa cacctacgct
    34981 tccccggaac agtggcagga aaaagccgaa accctggcga aactgtttat cgacaacttc
    35041 gataaataca ccgacacccc tgcgggtgcc gcgctggtag cggctggtcc gaagctgtaa
    35101 tcagctgata tctcattgac cccttatcca aacggataag gggttttttt atatctaatt
    35161 agctaccaat atgtacacgg tacacttata attatcaata gaccgcagga tgcattatgt
    35221 tcacctttat tgaactacaa ggatttagca aacgccgcca gttattgcta cctgatgatg
    35281 agttccggat ttttcaggaa ctactcattc aagatcccac tgccggtgac attatcgcag
    35341 gcacaggcag ttttagaaaa attcgctgga accgttctgg aatggggaaa cggggtggca
    35401 ttcggataat ttattacaac gtaacacgca atggccgaat ttatctggcg ttgatttatc
    35461 ctaaaaatga acaggatgat ttaacagaag aacaaagaaa ggcattgaaa ctattgtccg
    35521 agaagctgct ttaatgttga agctccacaa attccaccat ggtcgaaatg ccaacggaga
    35581 cattgaaaac agatgaaaga tgaattattt gctgatcttc ttgccagcgc agaagaaatg
    35641 gtacgcatcg aaaagggtga agaaacgcct gaaccagagc acgtccatac ttttagcgag
    35701 atcgatgtaa aagcgatccg tgaagcaacg ggtttacgtc agcaagattt tgctgtcgcc
    35761 gttggggtaa gttatgacct ggtcaaaagc tgggaaacca aacgccgtca acctaccgga
    35821 gcacctcgaa aattgctcct gcttttgcaa gcgaatcctt tcattatcaa tcaattgaaa
    35881 acaatctaaa aacgggaggc accttcgcct cccgtttatt tacccttctt ttgtcgtgcc
    35941 ctgcgcccgc gttaccggca ctggcagcca ggcgcgaatg gaaagcccgc cccgctcgct
    36001 ggtgccaagc tccagcatcc cgttatggtt atccacgata cgctgcacaa ttgccagccc
    36061 taatcccgtg ccgctaatgg tgcgcgcact gtcgccgcgg acaaacggct ggaacaggtg
    36121 cttacgttgt tccggcgcaa ttcccggacc gtcatcttcc acctggaacc aggcgcgatt
    36181 cggctccgtt ccgctgctga ctttgatcca gccattgcca taacgggcgg cgttgaccac
    36241 catattcgcc accgcgcgtt tgatcgacag cgggtgcatt ttcacttcaa tgctgccggg
    36301 gtaaagcgcg gtttcaattt cccgctcata gccactttcg gcagcaatca cctcaccgag
    36361 tactgcatta agatccgcca tttccatcgg catctcctgc ccggtgcgca ggtagtcgat
    36421 aaactgctca atgatggcgt tgcactcttc gatatcttta ttgatcgatt ctgccagata
    36481 gccatcctgc tcgctcatca tctcagtcgc caggcgaata cgcgtcagcg gcgtgcgcaa
    36541 gtcgtgactt acccccgcca tcagcagcgt gcggtcatcc gccagttgct taacaccagc
    36601 cgccatatgg ttaaaggcac gggtaacgga acgcacctcg gacgcgccat actcacgcag
    36661 cggcggcgga ataatccctt taccaacctg caaggctgcg tgttcgagat cgaccaacgg
    36721 tcggttctgg atacgaataa acagccacgc cccgcctatc gccaatagca taatcgccag
    36781 cgtatagcgg aacagcggag agaaatcgcc ctgatgaatt tcggtcagcg gcacgcgtac
    36841 ccagatattg ggcgacagcc aggttttcag ccagacgaca ggcgaacttt tgttgacctc
    36901 aacgcgcact tccgtcgggc cgcccagttg ctgcgccatc tgatggctta agaattcata
    36961 gtgttgcgcc caacgcagac ctgcctcttc ggcagcctcg ttggagtaga gagagatccc
    37021 cagctcacgg tagatctccc gacggaaagc gggaggcaca accaactgcg tgccgtcctc
    37081 cagttgcagt ttgtcggtca tcaacatacg cacttcgtac gcgaggactt tattaaactg
    37141 ctggaggctc ggcaaaatcg cgaagttcag caccaccaga taagtcgtca ccaggctggc
    37201 gaacagcaag gtgacgatga gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg
    37261 caatcgcctc atgctttaga gccgtccggt acaaagacgt agcccagacc ccagacggtc
    37321 tgaatgtaac gcggatgcgc tggatcttct tccaccatgc ggcgcagacg cgaaatctgc
    37381 acgtcgatgg agcgttccat tgcggaatat tcacgaccac gggcaaggtt catcagctta
    37441 tcgcgggaga gcggctcacg cggatggctg accagtgcct tcagtaccgc aaactcaccg
    37501 ctggtgagcg gcatcggctc gtcttcgcgg aacatttcgc gcgtaccgag gttaagtttg
    37561 aacttaccga aagcaattac cgcctcttcc tgtgacggtg cgcctggcag ttcgttcgcc
    37621 tgacgacgca gcaccgcacg gatacgggcc agcagttcac gcgggttaaa cggttttgga
    37681 atgtagtcgt cagcgccaat ctccaggcct acgatacggt ccacttcttc ccctttcgcc
    37741 gtcaccataa tgatcggcat cgggttgctc tgactacgaa gacgtcggca aatcgacaag
    37801 ccatcttcac caggtaacat taaatccagt accataagat ggaaagattc acgagtcagc
    37861 aggcgatcca tctgttctgc attagcgacg cttcgaacct ggaagccttg ttcggtgaga
    37921 taacgttcca gcagcgcacg caggcgcatg tcgtcatcga ccaccagaat cttgtagttc
    37981 tcttgcattg tttgtactcc caaaggttcg caacaatttg taagcgtgta ttcttaaaaa
    38041 agctcacgtt cgtcaccagc taaatctggt atgaatttca gcctaaattg ttacaaagca
    38101 tattaaacag cagcttaagt atacaattta ttcggcgaaa cattattgat tctgttgata
    38161 tgatcacgtt atacccaatg tgcgcattat caaacagaca aagggaatca acgagatgaa
    38221 aacgcccctg gttacccggg aagggtatga aaaactcaaa caagagctta attatctctg
    38281 gcgtgaagaa cgcccggagg tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg
    38341 cagcgaaaat gctgactatc agtataataa aaagcgtctg cgtgaaatcg accgtcgcgt
    38401 gcgctatctc actaaatgcc tggaaaatct caaaatcgtc gattactccc ctcagcagga
    38461 aggcaaagtc ttttttggcg cgtgggtgga gattgaaaac gacgatggcg tgactcaccg
    38521 tttccgtatt gtcggctacg atgaaatttt tggccgtaaa gattacatct ctatcgattc
    38581 cccgatggcc cgcgcattgc tgaaaaaaga agtcggcgat ctggcggtgg tgaatacccc
    38641 tgccggggaa gcgagctggt atgttaatgc tatcgagtac gtgaaaccgt aaggaagagt
    38701 cttaacctcc tgccgatggc tggcattttt gccagccagt ccgtataact atcccctgat
    38761 ttttgatccg aaaagatgaa ctcaaaccat gatgaatgat tcgttctgcc gcattattgc
    38821 gggtgaaatt caggcgcgcc cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg
    38881 gaataccgtg ccgtttatcg cacgttatcg taaggaaatc accggcggtc tggatgacac
    38941 gcagctgcgt aatctggaaa cgcgtctgag ctatctgcgc gagctggaag agagacgtca
    39001 ggcgatcctc aagtccattt ccgagcaagg caaactcacc gatgatctgg cgaaggccat
    39061 caacgccacc ctaagcaaaa ccgaactcga agacctctac ctgccctaca aacctaaacg
    39121 ccgcacccgc gggcaaatcg ccattgaagc agggcttgag ccgttggctg acctgctgtg
    39181 gagcgatccg tcacacacgc cagaagtcgc cgctgcacaa tatgttgatg ccgataaagg
    39241 cgtggcagat accaaagccg cgctggatgg cgcgcgctat atcctgatgg aacggtttgc
    39301 cgaagatgcc gcgctgctgg cgaaagtgcg tgattatctg tggaagaacg cgcatttggt
    39361 ttctacggtg gtgagcggta aagaagagga aggggcgaaa ttccgcgact atttcgatca
    39421 tcacgaaccg ttgtccacgg tgccttctca ccgcgcgctg gcgatgttcc gtgggcgtaa
    39481 cgaaggcgta ctccagcttt cgctgaatgc cgatccgcag ttcgatgagc cgcccaaaga
    39541 gagctattgc gagcaaatca tcatggatca ccttggcctg cgcctgaaca atgccccggc
    39601 ggatagctgg cgcaaaggcg tagtgagctg gacctggcgc atcaaggtgc tgatgcatct
    39661 ggaaaccgaa ctgatgggta ccgtgcgcga acgcgcggaa gatgaagcaa tcaacgtctt
    39721 tgcccgtaac ctgcacgatc tgctgatggc ggcccctgcc ggactgcgtg caacgatggg
    39781 cctcgatccg ggtctgcgta ctggggtaaa agtggcggtg gtcgatgcca ctggcaaact
    39841 ggtagcgacc gataccattt acccgcacac cggacaagcc gcaaaagcag cgatgaccgt
    39901 tgctgccttg tgtgaaaaac ataacgttga actggtggcg atcggcaacg gtacagcttc
    39961 ccgcgaaact gaacgtttct atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca
    40021 gaaagtgatc gtcagcgaag ctggcgcgtc ggtttactca gcttccgagc tggcagcgca
    40081 ggagttcccg gatctcgacg tttcgctgcg tggcgcggtg tctatcgccc gccgtttgca
    40141 ggatccgctg gcggagctgg tgaaaatcga tccgaaatct atcggcgtag gtcagtatca
    40201 gcatgacgtc agccagacgc aactggcccg caaactggac gcagtagtag aagactgcgt
    40261 aaacgccgtt ggcgtcgatc tcaacactgc ttctgttccg ctgttaaccc gcgtggcggg
    40321 cctgacgcgc atgatggcgc aaaacatcgt ggcctggcgc gatgagaacg gccagttcca
    40381 gaaccgtcag caactgctga aagtcagccg tctggggccg aaagccttcg agcagtgcgc
    40441 gggcttcttg cgcattaacc acggtgataa cccgctggat gcttctaccg tccacccgga
    40501 agcctatccg gtggtggaac gcattctggc agcaacacag caggcactga aagatctgat
    40561 gggtaacagc agcgaactgc gtaacctgaa agcgtctgac tttactgatg aaaaattcgg
    40621 tgtgccgaca gtaactgaca tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc
    40681 ggaatttaaa accgctcagt ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg
    40741 tatgatcctc gaaggtgcgg tgaccaacgt caccaacttt ggcgcgtttg tcgatatcgg
    40801 cgtgcatcag gacggcctgg ttcacatctc ttcattgtcg aacaagtttg tggaagatcc
    40861 gcataccgtg gtgaaagcgg gcgacattgt gaaggtgaaa gtgctggaag tggatcttca
    40921 gcgtaaacgt atcgccctga ctatgcgtct ggacgagcag cctggcgaaa ccaacgcccg
    40981 tcgcggcggc ggtaatgaac gcccgcaaaa caaccgcccg gcagccaaac cacgcggtcg
    41041 tgaagcgcag cctgccggta atagcgcgat gatggatgcg ctggcggcgg caatgggcaa
    41101 aaaacgttaa acgcccggac tggcccctaa acctgcgcga taaggcgttc acgccgcatc
    41161 tggcaacgaa cgccttgcct gacataaaag tgccggagaa tatctccggc ctttttattc
    41221 cacaaccaaa ctcataatat attccggcaa tatttatcac ttcattaaca actgaaacct
    41281 taattaaaca ttagccagtc cgggtaattc actattcgaa ttatattttc gctgcgatat
    41341 aaccttgagc cacatcaaca ttgagtcaga ttattattca aaccaacatt cgcacacatt
    41401 ttaagtattg ctgatagaaa ccattctcat tatcattgtg ttgttgatta tttaatctct
    41461 cctttgttgg caaatcatct ggtctcatgt cgctgtcaaa cgccccatga ggtagttatc
    41521 cagttaatga gaaacaagta ggcacctatg caatacactc cagatactgc gtggaaaatc
    41581 actggctttt cccgtgaaat cagcccggca tatcgccaaa aactgctttc tcttggcatg
    41641 ttacctggct cctcttttaa tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc
    41701 gaaacccgtc gtgtgagcct ggtattacgc aaaaaagatc tggccttatt agaagtggaa
    41761 gcggtttcct gttaatacgg tgataacaac aatgaaaaaa ttaaccatag gcttaattgg
    41821 taatccaaat tctggcaaga caaccttatt taaccagctc actggcgcac gtcagcgtgt
    41881 aggtaactgg gctggcgtta ccgtcgaacg taaagaaggg caattctcca ccaccgatca
    41941 tcaggtcacg ctggtggacc tgcccggcac ctattctctg accaccatct catcgcagac
    42001 ctcgctcgat gagcaaatcg cctgtcacta cattttgagt ggcgacgccg acctgctgat
    42061 taacgtggtg gatgcgtcta accttgagcg taacctgtac ctgacgctac aactgctgga
    42121 actcggcatt ccctgcattg tggcactgaa catgctcgac attgccgaga agcaaaatat
    42181 tcgtattgaa attgatgctc tgtcggcgcg tctgggctgt ccggtgatcc cgctggtttc
    42241 aacccgtggt cgcggtattg aagcgctcaa gctggcgatt gatcgctata aagctaacga
    42301 gaatgtggaa ctggtgcatt acgcacagcc gctgctcaac gaagcagatt cactggcaaa
    42361 agtgatgcct tccgacatcc cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga
    42421 aggcgatatc tacagccgcg cctacgccgg tgaagcgtcg cagcatctgg atgccgccct
    42481 cgcccgtctg cgtaatgaga tggacgatcc ggcgctgcac attgccgatg cgcgttacca
    42541 gtgcattgct gccatctgtg atgtggtaag caacaccctg acggcagaac ccagccgttt
    42601 caccactgcg gtagataaaa tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt
    42661 tgtgatgtac ctgatgttcc tgctggctat caacatcggc ggggcgttac agccgctgtt
    42721 tgacgtcggc tccgtggcgc tatttgtgca tggtattcaa tggattggct acacgctcca
    42781 cttcccggac tggctgacta tcttcctcgc ccagggcctg ggtggcggca ttaacaccgt
    42841 gctgccactg gtgccgcaga ttggcatgat gtacctgttc ctctccttcc ttgaggactc
    42901 cgggtatatg gcgcgtgcgg cgtttgtgat ggaccgtctg atgcaggcgc tgggcttgcc
    42961 ggggaaatcc tttgtgccgc tgatcgtcgg tttcggttgt aacgtaccgt cggtaatggg
    43021 tgcacgtacg cttgatgcac cgcgtgaacg tctgatgacc atcatgatgg caccgtttat
    43081 gtcctgcggc gcgcgtctgg ctatcttcgc agtattcgcg gctgccttct tcgggcagaa
    43141 cggtgcgctg gcggtcttct cgctgtatat gctgggtatt gtgatggcgg tgctgactgg
    43201 cctgatgctc aagtacacca tcatgcgcgg tgaagcgacg ccgtttgtca tggagttgcc
    43261 ggtctatcat gtaccacacg ttaaaagcct gattatccag acctggcagc gtctgaaagg
    43321 cttcgttctg cgtgctggta aagtgatcat catcgtcagc attttcctga gcgctttcaa
    43381 cagcttctcg ctgagcggga aaatcgtcga taacatcaac gactcggcgc tggcgtccgt
    43441 cagccgggtg atcaccccgg tcttcaagcc aattggcgtg catgaagata actggcaggc
    43501 aacggttggc ctgtttacag gtgccatggc gaaagaagtg gtagtgggta cgctcaacac
    43561 cctctacacc gcagaaaata ttcaggacga agagttcaat ccggcagagt ttaacctcgg
    43621 tgaagagctg ttcagtgcga tagatgaaac ctggcagagc ctgaaagaca ccttcagcct
    43681 tagcgtactg atgaacccca ttgaagccag caaaggcgac ggcgaaatgg gtaccggggc
    43741 gatgggcgtg atggatcaga aattcggtag cgcagcagca gcttacagct acctgatttt
    43801 cgtcctgctg tatgtaccat gtatctcggt gatgggagcc atcgcccgtg aatcaagccg
    43861 tggctggatg ggcttctcca tcctgtgggg gctgaatatc gcttactcgc tggcaacatt
    43921 gttctatcag gtcgccagct acagtcagca cccaacttac agcctggtgt gcattctggc
    43981 ggttatcctg tttaacatcg tggttatcgg tctgctgcgc cgcgcgcgta gccgggtgga
    44041 tatcgaactg ctggcaaccc gcaagtcggt aagcagttgc tgcgcagcca gcaccaccgg
    44101 tgattgccat taatggcttc acttattcag gtgcgcgatt tgctggcgtt acggggccgt
    44161 atggaagcgg cccagataag ccagacattg aacactccac agccaatgat taacgccatg
    44221 ctgcaacaac tggaaagtat gggcaaagcc gtgcggattc aggaagaacc tgacggctgc
    44281 ctctctggca gttgtaaaag ctgcccggaa ggaaaagcct gtctgcgcga gtggtgggcg
    44341 ctgcgttaac cttactccat cgcctgtttt ggaaagcccg gtatgcgtct gcatccgggc
    44401 ttttttgcgt gaggctttcc ataaaaatgc aactcttgca gcacggcgta agttcctttg
    44461 aaagcatctc gcagggatga aaactcgcta atgcacaggt gtggagtggc gcgtagagtc
    44521 gcggcattca aacaacaggt gaaggaacgc catgagcaaa aagcagagtt ccaccccaca
    44581 cgatgcgctg ttcaaactct ttttacgcca accggacacg gctcgtgatt ttcttgcgtt
    44641 tcatttaccg gcacccattc acgcgctttg tgatatgaaa accctcaagc tggagtcgag
    44701 cagctttatt gatgacgatc tgcgtgaaag ctattccgat gtgctgtggt cggtgaaaac
    44761 ggaacaagga ccaggataca tctattgtct gattgaacat caaagcacct caaacaaact
    44821 gatcgcattt cgcatgatgc gttacgctat tgccgcaatg caaaatcacc ttgatgctgg
    44881 atacaaaacg ttgccgatgg tggtgctatt gttgttttac cacggtattg aaagccccta
    44941 tccctattcg ctgtgttggc tggattgttt cgccgatccc aaactggtaa ggcagcttta
    45001 tgcctccgca tttccgctga ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca
    45061 ccgacgtatg gcgctgctgg agttaattca aaaacatatt cgtcaacgcg atctgatggg
    45121 gctggtagaa caaatggcct gcttattaag tagtggatac gctaatgaca gacaaatcaa
    45181 agggctgttt aattacatct tgcaaaccgg cgatgcggta cgttttaacg attttatcga
    45241 cggcgttgcc gaacgttcac cgaaacacaa ggagagttta atgactattg cggaaagatt
    45301 gcggcaggag ggggaacaat ccaaagcctt gcatatagcc aaaataatgc ttgaatccgg
    45361 agtccctctt gcagacatca tgcgctttac cgggctgtca gaagaagagt tggctgcggc
    45421 gagtcagtaa agttctgtct cgccattcaa aagtgcctac accctctgct tcaacgccac
    45481 cagcacgtga cgaaactcga ccggatgcga aataaacggc gcatgagccg ctttggcgaa
    45541 gatgtatgac tcgctgtgtg gccagagttt atccagcatc ggcaccactt ttcgcggcac
    45601 cagaccatcg agatagccat acaatcgcaa aaacggcatg gacacgtttt gcagcggctg
    45661 gcggagatcg accgttttca ggatttccag cccgccatta agcacgtcaa cctccggcat
    45721 cggtaacgcc agaacggttt tcttcaacgc ccgcgcatcc tgacgcgccg tttcagtacc
    45781 catggtttgt aacgcaagga accgttccac cgtacgctga aaatcatcac tgagttgctg
    45841 ctgaaatccc gccagcacgt ccggttttat ccccggccac tcgtcacgag cactaaaaca
    45901 aggtgacgac gccacggtga ccagcgcctg aacacgctcg ggatgggtta acgcaatctg
    45961 gcttgccacc agcccgccca gactccagcc taaccaaatg gctttatcag gtgcctgtcg
    46021 cagcacggct tcggccatat cagcaagtga catcgcacca aatccccggc tacgcccgaa
    46081 gccgggcagg tcaacaaggt gcagcgtaaa atgcgagctc agttcctcgt caatgcaacg
    46141 ccacacttcg gcattcagtc cccatccgtg cagcagcaca agatgaacat tcccctgacc
    46201 tttggtctgc caccagatgt tattcatccg ctattgttct cttttgactt acaaggatga
    46261 acatatgcta acagtaccag gattatgctg gctatgccga atgccgctgg cgttaggtca
    46321 ttgggggatt tgttcggtct gctcacgcgc cgcccgtaca gataaaacgt tatgcccaca
    46381 atgtggatta cccgccacac actcacatct tccctgcggt cgctgcctgc aaaaaccacc
    46441 gccctggcaa agactggtca cggttgccga ctatgcgccg ccgttaagcc cgctaatcca
    46501 ccagcttaag ttttcccggc gcagcgaaat cgccagcgcc ctgtcacgtc tgttactatt
    46561 ggaagtctta cacgctcgtc gcaccaccgg gttgcaattg ccggatcgta tcatcagcgt
    46621 tccgttatgg cagcggcgtc actggcgtcg gggatttaat cagagcgatt tgctgtgtca
    46681 gccgttatca cgctggttgc actgccaatg ggatagcgaa gccatcacac gaacacgggc
    46741 cactgcgacc cagcattttc tcagtgcccg gctacgcaag cgcaacctga aaaatgcctt
    46801 tcgtcttgaa ttgcccgtgc aaggtcgcca tatggtgatt gtggatgatg tcgttaccac
    46861 cggaagtacc gtcgcagaga ttgcgcagtt gcttttacgc aatggtgcgg cgactgtcca
    46921 ggtctggtgc ctttgtcgaa ccttgtagag cctcgatgat gggcgtatta taaccaacta
    46981 aaatagtcaa ctattaggcc attactatga tccgtatttc cgatgctgca caagcgcact
    47041 ttgccaaact gctggcaaat caggaagaag ggacacaaat ccgcgtattt gtgattaacc
    47101 ctggcacgcc taacgctgaa tgtggtgttt cttattgtcc gccggatgcc gtggaagcca
    47161 ccgacaccgc cctgaaattt gacctgctga ccgcgtatgt tgatgagtta agcgcaccat
    47221 acctggaaga tgcagagatc gattttgtta ccgaccagtt gggttcccag ttaacgctga
    47281 aagccccgaa cgccaaaatg cgtaaagtgg cagacgatgc accgctgatg gagcgcgtgg
    47341 agtatatgct gcagtcgcag atcaacccgc aacttgctgg tcacggtggt cgcgtttcgc
    47401 tgatggaaat caccgaagac ggttacgcca ttctgcaatt tggcggcggc tgtaacggtt
    47461 gttccatggt ggatgtgacg ctgaaagaag ggatcgagaa gcagctgcta aacgaattcc
    47521 cggagctgaa aggtgtgcgc gatctcaccg aacaccagcg cggcgaacac tcctactact
    47581 aagttatccc ctcttttgta gattgcccga tgcgacgcta aagcgtcatt tcgggcctac
    47641 attgaacatg tgccagacgt tggcgcagca ttgctttggc aaatccctcc cttcgtttcc
    47701 ccgcgataat atgaccaacc tctcataatt taaatttacc ccgctctggt gattctcaaa
    47761 cgccagatgt tacccgtatc attcacatgg gtaccaaaca tactcctgac atctgactac
    47821 aataattagt tttagtgggt atcagtcgtg gtgccgcaat atctctgttc ccgattggga
    47881 taattagagt ttgtcgtcag aaaattgacg ttacccataa caaatgaaag gccaggtaaa
    47941 tcatgccatt agtcattgtt gctatcggtg taatcttgtt gttgctcctg atgatccgct
    48001 tcaaaatgaa cggcttcatc gctctcgtcc tcgtggcgct tgctgttgga ttaatgcaag
    48061 gaatgccgct ggataaagtt attggctcca tcaaagccgg tgtcggcggg acgctcggta
    48121 gccttgccct gatcatgggt tttggcgcaa tgctgggcaa aatgctggca gactgcggtg
    48181 gcgcacaacg tatcgccacc acgctgattg ccaaatttgg taaaaaacac atccagtggg
    48241 cggtggtact gaccggtttt accgttggtt ttgccctgtt ctatgaagtg ggctttgtgc
    48301 tgatgctgcc gctggtgttt accatcgcgg cttctgcgaa tattccactg ctgtatgttg
    48361 gtgtaccaat ggcggctgca ctgtctgtga cccacggctt cctgccaccg catccgggcc
    48421 cgactgcgat tgccaccatt ttcaatgccg acatgggtaa aaccctgctg tacggtacta
    48481 ttctggcaat cccgaccgtg attctcgccg gtccggttta cgctcgcgtg ttgaaaggta
    48541 tcgataagcc aattccggaa ggtctctaca gcgcgaaaac cttcagcgaa gaagagatgc
    48601 cgagctttgg cgtcagcgtc tggacctctc tggtgccggt agtactgatg gcgatgcgtg
    48661 cgattgccga aatgatcctg ccgaaaggtc acgctttcct gccggtagcg gagttcctcg
    48721 gtgacccggt aatggctacg ctgattgccg tgctgattgc gatgttcacc tttggtctaa
    48781 accgtggtcg ttcaatggat cagattaacg acacgctggt ttcttccatc aaaatcattg
    48841 cgatgatgct gttgatcatc ggtggtggcg gtgcgttcaa gcaggtgctg gtagacagcg
    48901 gcgtggacaa atacattgct tccatgatgc acgaaaccaa catttctccg ctgctgatgg
    48961 cctggtcgat tgctgccgta ctgcgtatcg cgctgggttc cgcaaccgtt gcggcaatca
    49021 ctgcgggtgg tatcgcggca ccgctgattg caacgacggg tgttagcccg gagctgatgg
    49081 ttattgcggt tggttccggt agtgtgattt tctctcatgt gaacgatccg ggcttctggc
    49141 tgttcaaaga gtactttaac ctgactatcg gcgagaccat caaatcctgg tcgatgctgg
    49201 aaaccattat ctcggtgtgc ggtctggtag gctgtctgct gctgaatatg gtgatttgat
    49261 aacactgact gccggatgcg gcgtgaccgc cttatccggc ctacgattcg ggatgaatta
    49321 gtaggccgga taagacgcgt caagcatcgc atccggcatc aaccgcactc tacttcttct
    49381 tcgctgcagc tctgcgccgt ctgtccaaat ccttcagcaa cttattcacg ccatcatcgg
    49441 caaacatcgt ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac tggtaactgg
    49501 tgccaggaat attcaccggt tcggccatat ccagccagtc ttccggctgt agtcctaaca
    49561 gagcactgtt actgtcggca atgtagcgct gcaaaccacg attaagcgtc ggcgtcatcg
    49621 acatcaacga tgccttatgc ccggcacgtt ttggcagaca accatattta tgcagtgcat
    49681 ccagcagccc ttgcttcgcc agttcgcgat cctgatacag accgcgcagt accacttcat
    49741 ccggatacag ccccagggtt ttgcccagcg ttagatcccc gctctcccag tagccgcgta
    49801 gcgtaggcag gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac gctttcggtg
    49861 cacggaacgt cttctcgtgg tcgttttcga aatagagcac tttgtaggag tacacaccgc
    49921 tgctgcgcag cttaccgaca atctctaccg gtacggtacc gagatcttca ccaatcacca
    49981 tacagcgatg acgtttactt tcgagtgcca gaatcgagag cagatcatcc accggatagt
    50041 gaacatacgc gccctgatct gccgtctcgc catacggtat ccaccacaaa cgcagcatcg
    50101 acatcacatg gtcaattcgt aatgcgccgc agttttgcat attggcacgc aacagctcga
    50161 taaacggttc ataggcacgc gcggtgatga tatgcgggtc cattggcggt aatccccagt
    50221 tctgccccaa cgggccgagg atatccggcg gcgcgccaac cgacgctttc aggcaataca
    50281 gttcacggtc gcaccaggtt tccgccccac cttccgctac gccaaccgcc agatcacgat
    50341 acaagccaat cggcatttca tagccctggc ttatctccca gcaggcggca aactggctgt
    50401 aagccagcca ctgcaaccag agataaaaat cgacgtcatc acgatgttct tcgcagaact
    50461 gacgcacttc tggtgaatcc acgttctgat acatctctgg ccatgcaggc cagccccagc
    50521 gcatttcgtc ctctttcact tgctgggcat gtagcgcatc aaaggctgcc tgccagaaca
    50581 ggctgtcgcc ttgatctgca acaaactggc gaaacgcggt catctgctca tcatcacgtt
    50641 gcgcgaaacc tttccacgcc attcgtaatg ctgtcatttt tagggcggta accgtggagt
    50701 aatcgaccca gtcggcatcc cgcgcctgtt gcagcgtctg ttgcgtggtc ggcaactgcc
    50761 accaggcctg agcctcttcg ctaagatgga aatcttcgac ggcgttaacg tcgatataaa
    50821 tcacattcag ccaacggcga gaagacgggc tgtatgggct ggcactctcc ggatttgccg
    50881 gatagagcgc atgaatcggg ttcaggccga tgaacgaccc gccacgtttt gccacatcca
    50941 ccagcatcgc tttgagatcg ccaaaatccc caatacccca gtttttttcc gatcgcagcg
    51001 tataaagctg aacgcaggca ccccacagct tttgtttatt cagcaacgcc tgcggttcgt
    51061 aacagcgttt cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc tgggtgagtg
    51121 tcagcgtgtg ataaccttcc ggcagcttcg tcggtagatt gaacgctttg cccccagtta
    51181 catggccttt gtactgcgtt ccttcttcgg tggtcagcag ccagctatat tcgccgctgc
    51241 cctccaccac catcggcatt tttttgccgc tggtataaac catgacattc gggactggcg
    51301 ttaccgccac tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc cgtttggttt
    51361 cggcgctaat cgactgcggt ttaccgtggg cattgatgta attggggcta atccccgccg
    51421 ccagcgcggc attatccaga cgtttgcttt ccatcgagct tccttagcgt tttgcctgcc
    51481 agatacgagc ctgataatcg cgaatagagc gatccgagct aaacataccg cagcgggcgg
    51541 tattgaggat cgccgcgcga gtccaggcct cctggtcgcg gtacagcaca tccacctgct
    51601 tttgtgcctc tacataagct gcgaaatccg ccatcaccag atacggatcg ccgccctgtt
    51661 tgccgatact gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg tatttgccgc
    51721 tttccagctc tttcaatact gcgtccagca ccttatcttt cttccgccat ttcaccgggt
    51781 cgtagccttt ggccagaatt gccttcactt gttccacggt atgaccaaaa ataaagatat
    51841 tttcttcacc gactttctcg gcgatttcaa cgttcgcccc atccagcgta ccgacagtaa
    51901 gcgcaccatt gagcgccagt ttcatattgc cggtaccgga agcttcttta cctgcagtcg
    51961 aaatttgttc ggagatatcc gccgccggga tcagtttttc cgccgccgaa acgcaataat
    52021 ccggcaggaa caccaccttc aacttatcgc caaccagcgg atcgttgttg atcacgtcag
    52081 ccactttgtt gatcgcaaag ataatattct tcgccaggta gtagcccggt gccgctttcg
    52141 cgccgaagag gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga atttctttgt
    52201 acaacgccag aatatgcagc agattcaggt gctggcgttt gtactcatgc agacgtttga
    52261 tctgaatatc gaaaatcgcc tgtggattga tctcaatacc ggtacgaact ttcacaaact
    52321 ccgccagacg gactttattc gcctgcttga tctcgcgata ttgctgacgg aatttcgcat
    52381 catcagcgaa tttttccaga ttgatcagct gatcgagatc gttagcccac tcttttttca
    52441 gtgatttatc caacagagcc gccagtgccg ggttgcactg tttgatccag cgacgtgggg
    52501 taataccgtt ggtgacgtta tggaatttgt tcggccatag ctggtgatat tccgggaaca
    52561 gatctttcac caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg aaaccgccaa
    52621 ccacacacag gttcgccata tgcacttgtt tatcgtgcac caccgccagt ttggcccaca
    52681 ctttttcatc gcccggccag gttttctcca ccagcgtttt aaagcgagta ttaatttcgt
    52741 taataatctg catatggcgc ggcagtaagc ctttcaccag tttcacctcc cagcgttcca
    52801 gcgcttctgg catcagggta tggttggtgt aagcgaaagt tttgctggta atggcccagg
    52861 cgtcatccca gctcatctgg tgctcatcga tcagcacgcg cagcagttct ggaatcgcga
    52921 tagttgggtg ggtatcgttc agctgaataa cttcgtaatc cgccagttcg tgcagtttac
    52981 gccccgccag atgatgacga cgcaaaatat ccgctaccga acaggcacac tggaagtact
    53041 gctgcatcaa gcgcagcttt ttaccggcag tatggttgtc gtttggatag agaactttgg
    53101 tcagtttttc cgcattgatg ccctgctgtt cggcacgcag gaaatcaccg tcgttaaatt
    53161 tagtcagatc aaacggatgc gcgtgcgtcg cctgccacag acgcagcggc tgcgccacgc
    53221 cattacgata gccgacaacg gggagatccc acgcttgacc ggtaatggta aactccggct
    53281 cccagcgtcc gtctttcgtc actttaccgc caatccctac ctgcacatcc agtgcttcgt
    53341 tgtggcggaa ccacgggtag ttactgcgat gccagtcatc cggcgcttca acctgtttgc
    53401 catcgacaaa agactggcgg aacaaaccat attgatagtt cagaccgtaa cccgtcgcag
    53461 actgaccgac agttgccatt gagtcgagga agcacgccgc cagacgtccc agaccaccgt
    53521 tacccagcgc cgggtcgatc tcttcttcca gcaggtccgt cagattgatg tcataagcct
    53581 tcaacgaatc ctgtacatcc tgataccagc cgagattcaa caggttgttg cccgtcaggc
    53641 gaccaatcaa aaactccatt gagatgtagt taacatgtcg ctgattcgcc accggcttgg
    53701 cgaatggctg agcacgcagc atttcggcca gtgcttcact cactgctagc caccactggc
    53761 gaggagtcat ttcagccgca gaatttaagc cataacgctg ccactgacgt gaaagcgctt
    53821 cctgaaattg cttatcgtta aaaataggtt gtgacatagg agttccactt ttcttagatt
    53881 ttcaacacaa cgttatcgct agtttgccag gctcgatgtt gaccttcctc atcctgcggg
    53941 ggattaggca gggaggagtt gcggggatga gcaaggaaat gtgatctcaa ccacttaaag
    54001 ctagtgcaaa ccacaggatt agcagcaaat caatgcaata cagcgcagaa aatctgtatc
    54061 taagtgcaaa aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc tataaattaa
    54121 gtaaaggagt gaaacagttt cataagtaaa atatccagtg tgctccatct cattcttaat
    54181 agatttatta agatcatctt tttagatggc actttcatca ggaatgaaga agaaaccctt
    54241 gcttaaatga atctgatgaa cataagggaa accagtattc acgctggatc agcgtcgttt
    54301 taggtgagtt gttaataaag atttggaatt gtgacacagt gcaaattcag acacataaaa
    54361 aaacgtcatc gcttgcatta gaaaggtttc tggccgacct tataaccatt aattacgaag
    54421 cgcaaaaaaa ataatatttc ctcattttcc acagtgaagt gattaactat gctgattccg
    54481 tcaaaactca gtcgtccggt tcgactcgac cataccgtgg ttcgtgagcg cctgctggct
    54541 aaactttccg gcgcgaacaa cttccggctg gcgctgatca cgagtcctgc gggctacgga
    54601 aagaccaccc tcatttccca gtgggcagca ggcaaaaacg atatcggctg gtactcgctg
    54661 gatgaaggtg ataaccagca agagcgtttc gccagctatc tcattgccgc cgtgcagcag
    54721 gcaaccaacg gtcactgtgc gatatgtgag acgatggcgc aaaaacggca atatgccagc
    54781 ctgacgtcac tcttcgccca gcttttcatt gagctggcgg aatggcatag cccactttat
    54841 ctggtcatcg atgactatca tctgatcact aatccagtga tccacgagtc aatgcgcttc
    54901 tttattcgcc atcaaccaga aaatctcacc ctggtggtgt tgtcacgcaa ccttccgcaa
    54961 ctgggcattg ccaatctgcg tgttcgtgat caactgctgg aaattggcag tcagcaactg
    55021 gcatttaccc atcaggaagc gaagcagttt tttgattgcc gtctgtcatc gccgattgaa
    55081 gccgcagaaa gcagtcggat ttgcgatgac gtttccggtt gggcgacggc actacagcta
    55141 atcgccctct ccgcccggca gaatacccac tcagcccata agtcggcacg ccgcctggcg
    55201 ggaatcaatg ccagccatct ttcggattat ctggtcgatg aggttttgga taacgtcgat
    55261 ctcgcaacgc gccattttct gttgaaaagc gccattttgc gctcaatgaa cgatgcactc
    55321 atcacccgtg tgaccggcga agaaaacggg caaatgcgcc tcgaagagat tgagcgtcag
    55381 gggctgtttt tacagcggat ggatgatacc ggcgagtggt tctgctatca cccgctgttt
    55441 ggtaacttcc tgcgccagcg ctgccagtgg gaactggcgg cggagctgcc ggaaatccac
    55501 cgtgccgccg cagaaagctg gatggcccag ggatttccca gcgaagcgat tcatcatgcg
    55561 ctggcggcag gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc ctggagtctg
    55621 ttcaaccata gcgaactgtc gctgctggaa gagtcgctta aggccctgcc gtgggacagc
    55681 ttgctggaaa atccgcagtt ggtgttattg caggcgtggc tgatgcaaag ccaacatcgc
    55741 tacggcgaag ttaacaccct gctagcccgt gctgaacatg aaatcaagga catcagagaa
    55801 gacaccatgc acgcagaatt taacgctctg cgcgcccagg tggcgattaa cgatggtaat
    55861 ccggatgaag cggaacggct ggcaaaactg gcactggaag agctgccgcc gggctggttc
    55921 tatagccgca ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg
    55981 acccgctcac tggcgctaat gcagcaaacc gaacagatgg cacgccagca cgatgtctgg
    56041 cactacgctt tgtggagttt aatccagcaa agtgaaattc tgtttgccca agggttcctg
    56101 caaaccgcgt gggaaacgca ggaaaaagca ttccagctga tcaacgagca gcatctggaa
    56161 cagctgccaa tgcatgagtt tctggtgcgc attcgtgcgc agctgttatg ggcctgggcg
    56221 cggctggatg aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag
    56281 ccacagcaac agcttcagtg cctggcaatg ttgattcaat gctcgctggc ccgtggtgat
    56341 ttagataacg cccgtagcca gctgaaccgt ctggaaaacc tgctggggaa tggcaaatat
    56401 cacagcgact ggatctctaa cgccaacaaa gtccgggtga tttactggca aatgaccggc
    56461 gataaagccg ccgctgccaa ctggttgcgt catacggcta aaccagagtt tgcgaacaac
    56521 cacttcctgc aaggtcaatg gcgcaacatt gcccgtgcac aaatcttgct gggcgagttt
    56581 gaaccggcag aaattgttct cgaagaactc aatgaaaatg cccggagtct gcggttgatg
    56641 agcgatctca accgtaacct gttgctgctt aatcaactgt actggcaggc cggacgtaaa
    56701 agtgacgccc agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac cggatttatc
    56761 agccattttg tcatcgaagg cgaagcgatg gcgcaacaac tgcgtcagct gattcagctt
    56821 aatacgctgc cggaactgga acagcatcgc gcgcagcgta ttctgcgaga aatcaatcaa
    56881 catcatcggc ataaattcgc ccatttcgat gagaatttcg ttgaacgtct gctaaatcat
    56941 cctgaagtac ctgaactgat ccgcaccagc ccgctgacgc aacgtgaatg gcaggtactg
    57001 gggctgatct actctggtta cagcaatgag caaattgccg gagaactgga agtcgcggca
    57061 accaccatca aaacgcatat ccgcaatctg tatcagaaac tcggcgtggc ccatcgccag
    57121 gatgcggtac aacacgccca gcaattgctg aagatgatgg ggtacggcgt gtaagtttag
    57181 ccggataacg cgccagatcc ggcttacatc tctgcatcat tcaatgctca cccgcgttac
    57241 gccatctgtt tctatcaaac taaaccgcac cggcaagaaa cgctccacca ccgcgatatt
    57301 ggtcagcaga tggcatgagg gatgggcgac cgtaaattcc cccgcgcccg ccagcgccat
    57361 cggtagcacc agttggtcag caagatattc ccccaccgcc gccgtgcttg ccaggtagcg
    57421 tttcacctct ttcaccaact gtgccgcaac cacctcggca ctgacgcgct tttcaccgac
    57481 gacaaaaaag cgttcggtga tattttcact ttcgacttca agcgagacgg tattacccgg
    57541 cccctggtcg cgcggcaggt tatgaatatt ctgttcatgc agggaaaaac tccccgccag
    57601 tgtagcgatt tcacgctcag caacatggcg cggcacacca gctaatagaa cttctccacg
    57661 catctgcaca atgttcccgc gctcgccaag ttgcaaggtg ttaaacgatg ccacgggcga
    57721 gacttccgtt gccaccacac cgcctccggc agggtaaaaa ccgtggcgta acagcgtggt
    57781 ttgctgatga attcccattt tcgccagcag cggctccagc acccggcgga taaaatccgc
    57841 aggtggcgcc gacgggttat cggtaccgcc gctcacttca acacgcgaag gtccatcggc
    57901 aaaccacagc gcgggcagca cagtttgcag caccagcgta caacttccgg cgctaccgat
    57961 agcaaagcgg taatcgccgc cccgcacggt gccaggccgg aagagcagac gctgcgatcc
    58021 cagctccgca ccttccaccg ttgccctaca aatttccgca gccgctttta ccgcggtcag
    58081 atgctggcgc agcagccccg gtttcgcccg tccggctcga atgccagtga tggtaaatgg
    58141 ctggccggtt atcatcgaca ggctcagcgc cgagcgcagg atctgtccgc cgccttcgcc
    58201 ctgtgcgcca tccagcgcaa tcatcctttt catcatttat ccttttacgc acaccacctg
    58261 acgcagggtg tagataactt ccaccagatc gctttgtgcc gccatcaccg catcaatatc
    58321 tttatacgcc atcgggattt cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc
    58381 ggtggcacga atttgatctt ccacgctgaa cagttttttc gctttagtac ggctcattac
    58441 ccgcccggca ccgtggctgc acgaacagaa cgactcttca tttcccaacc cacggacgat
    58501 aaagcttttc gctcccatcg aaccgggaat aattccatat tgaccagcac gcgcagacac
    58561 cgcgccttta cgcgtcacgt agatctcttc accaaagtgc tgttcttttt gcacatagtt
    58621 gtggtgacag ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt
    58681 aatgctctgc aatgccgtta ccacgttttc catcatcgca tcgcggttaa ggctggcaaa
    58741 aagctgcgcc caggccacgg ctttcaggta atcatcaaag tattccgtac cttccataaa
    58801 gtacgccaga tcacgcgacg gcaacgtctc aagcgtttcc tgcatctctt tttgtgccag
    58861 atcgataaag taagtcccga tggcgttacc aattccgcgt gaaccggagt gcagcataat
    58921 ccacacctgc tccgactcat caaggcagat ttcaataaag tggttaccgg ttcccagcgt
    58981 tcccaggtgt ttatagttat tggtattcag gaaacgggga tatttttgcg ttaaccactg
    59041 ataaccggct tcaagctcag cccatttcgc atcgacgtta acaggcgggt tttcccatgc
    59101 gcctttatca cgtttacaac ggccagtggt acgcccgtgc ggcacggccg tttcaatcgc
    59161 ctgacgcagc tctgccaggt tttcaggcag gtctgccgcc gttaacgcgg tacgcagcgc
    59221 gttcattcca cagccaatat ccacgcccac cgccgccgga ataatcgccc ctttggtcgg
    59281 gatcacgcta ccaatggtgg aacctttacc caggtgtaca tcaggcatta ccgcaatgtg
    59341 tttgaaaata aacggcatct tcgccgtatt aataagttgc tgacgcgcat cggcttctac
    59401 cggcacgcct ttggtccaca tttttaccgg ggcattttca gtggtcagta attcgtaatt
    59461 cattttgttt ttctcttttc gttgtttgct gtcgtgataa ttgcaaccgt cgtgccagaa
    59521 aattaaataa gcagattaat tttttaattc attgttttta aataggttaa atctttacgt
    59581 ccgtaaacgg agattttcca caaaaccaat tcaccgataa tgaaatatcg tcttttataa
    59641 ggatatctaa gatgcgtaaa acagtggctt ttggctttgt cggaacagtg ctggattatg
    59701 ccgggcgcgg cagtcagcgc tggtcgaaat ggcttccgac actctgttta tgccagcaag
    59761 aatcgttggt catcgatcga ctggaattat tgcatgacgc ccgctcacgc tcgctatttg
    59821 aaacgcttaa acgcgatatc accagcgttt cgccagaaac agaagtggtg agcgttgaga
    59881 ttgaactgca taacccgtgg gatttcgaag aggtttacgc ttgcctgcat gatttcgccc
    59941 gtggttacga gtttcagcca gaaaaagaag actatttaat tcacattacc accggcaccc
    60001 acgtcgcgca gatttgctgg tttctgctgg cggaagcgcg ttacctgccc gcccggctga
    60061 tacaatcttc acctccgcgc aaaaaagaac agccgcgcgg cgcaggtgaa gtgacgatta
    60121 tcgatctcga tttaagccgt tataacgcca tcgccagccg ctttgccgag gaacgccagc
    60181 aaacgcttga ttttcttaag tccggcattg ccacgcgtaa cccccacttc aaccgcatga
    60241 ttgagcagat cgaaaaagtg gcgatcaaat cccgcgcgcc gattctgctt aacggtccaa
    60301 ccggcgcggg caagtcattt ctggcgcgac gcatcttcga gttaaaacag gcgcggcatc
    60361 agtttagcgg cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat accgccatgt
    60421 cgacgctgtt tggtcatgta aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggtt
    60481 tattacgcag cgccaacggc ggaatgttgt ttcttgatga gattggcgaa ctgggcgcag
    60541 acgaacaagc aatgctgcta aaagccattg aagagaaaac cttttacccg tttggcagcg
    60601 atcgccaggt gagcagtgat tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc
    60661 tggttgccga aggcaaattc cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca
    60721 ccctgccggg gctacgccag cgccaggaag atattgaacc taacctggat tatgaagtgg
    60781 agcgccacgc ctcactcacc ggcgacagcg tgcgttttaa caccgaagcg cggcgtgcct
    60841 ggctggcctt tgcaacatct cctcaggcga catggcgcgg taactttcgc gagctttctg
    60901 ccagcgtcac gcggatggca acctttgcca ctagcggacg catcactctg gacgtggttg
    60961 aagatgagat aaaccgtctg cgctataact ggcaggagag ccgtccctcc gcgcttacgc
    61021 aactgctggg cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg
    61081 ttatcgctat ctgccgccag gcaaagtcgc tttctgctgc cggacgacag ctttttgatg
    61141 tttcgcgcca gggcaaagcc agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc
    61201 gttttggtct gacctgggaa gcattgcagg atcagcacag ctccagttga atatggtggt
    61261 ccgtcagcac ctgcatcacg cttacaggcg gctgggcgtc ggtgtagacg gcatctacca
    61321 tgctgatgct gcccatattg accatcgcgt tacggccaaa tttcgagtga tcgacaacca
    61381 gcataacgtg gcgcgagttc tcaataatgg cgcgtttggt gcgaacttcg tggtaatcga
    61441 actccagcag cgagccgtcg ctatcgatgc cgcttatccc cagaatgccg aaatcaaggc
    61501 ggaactggga gataaaatcg agcgtcgctt cgccaatgat cccgccatcg cggctgcgta
    61561 attcgccacc ggcgagaatg atgcgaaaat cttcttttac catcaacgtg ttagcaacgt
    61621 tgagattgtt ggtgacaatg cgcaaattgc tgtgattgag cagtgcgtgc gctaccgctt
    61681 ccggcgtggt gccgatatcg ataaacagcg tcgagccatt ggggatttgc tccgccactt
    61741 tgcgggcgat gcgctctttt tcttcggtct gggtggcctt gcgatcgtgc cacggcgtgt
    61801 taaccgaact ggaaggcagc gccgcgccgc catgatggcg caggatcagg ttttgctccg
    61861 ccagctcatt gaggtcgcgg cgaatagtct gcgggctgac ggagaagtgc tccaccagtt
    61921 cctcggtact gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg
    61981 tttgtttcat ttctaaatcc ctggaattat tttcgttttc gcgcattaag cgaatcaaca
    62041 aaagccatcg ctaaacccac ggctaacccg gcgatgtgtg ctccgttcgc catcgacata
    62101 ccaaacaaat caaaccatcc ggcgacaatc cagatcagcg caaagataat taacccacgt
    62161 tgcaggtaaa tgccactttg cggatcgcgt tcgccacgta gccagacgta gcccatcagc
    62221 gcatacacca cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcacatag
    62281 ccgcttaaca gggcgctaat gagagtaata acaattagct taccgctacc gaggcgtttt
    62341 tccaccgcac cgccgagata ccaccaccag agcaggttaa agaggatatg catcagcgag
    62401 aagtgcatta acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt tggatcgaat
    62461 ggccaggcca gccataacat cacttcctga tcgccgagaa tttgcatggc gataaacacc
    62521 accacgcagg cgatcatcac cacccaggtt accggacctg cgcgttcacg caaggcagca
    62581 aagaaaggat aacggcgata atgcagacca ctgccggtat ggcccgcctg ccagctggcc
    62641 gccagataac gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgc
    62701 tcggcctggg actcatccgc cagccagaca tcgctttggt tatgttgttg aatcgtaagg
    62761 ataacaccct gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa
    62821 gaggtaatca tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc
    62881 gttacgcgcc gtacgccacc tctgcgggaa actgacgctg ccaggcttca aaaccgccgt
    62941 caatgctata gaccacatcg tagccctgtt gcagcagata ctgcgccgcg cctttgctgc
    63001 tattgccgtg ataacacatc accatcaccg gagtgtcaaa gtcgttatca cgcataaacg
    63061 cgcccagcgt gtcgttggtt aaatggaaag cctgcaccgc atgtcccatt gcgaaactct
    63121 gtggatcgcg aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg
    63181 caacgttaat acattcgaac tgatccatgc gtctctcttt ctttacaaac aagtgggcaa
    63241 atttaccgaa cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact
    63301 ctaaaatgtt ttttcaatgt tacctaaagc gcgattcttt gctaatatgt tcgataacga
    63361 acatttatga gctttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt
    63421 gatagggggc ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc
    63481 cgtgctgatg ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact
    63541 cattcacggt ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct
    63601 ggctgaacgt gaagtgctgc tgaaaatggc tccgcatatc gccttcccga tgcgttttcg
    63661 cctgccacat cgtccgcatc tgcgcccggc gtggatgatt cgcattggtc tgtttatgta
    63721 cgatcatctg ggtaaacgca ccagcctgcc gggatcaact ggtttgcgtt ttggcgcaaa
    63781 ttcagtgtta aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt gggtagacga
    63841 cgcccgtctg gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac
    63901 tcggactcgc gccacctctg ctcgccgcga aaacggcctg tggattgtgg aagcggaaga
    63961 tatcgatacc ggcaaaaaat atagctggca agcgcgcggc ctggttaacg ccaccggccc
    64021 gtgggtgaaa cagttcttcg acgaggggat gcatctgcct tcgccttatg gcattcgcct
    64081 gatcaaaggc agccatattg tggtgccgcg tgtgcatacc cagaagcagg cctacattct
    64141 gcaaaacgaa gataaacgta ttgtgttcgt gatcccgtgg atggacgaat tttccatcat
    64201 cggcaccacc gacgtagagt ataaaggcga tccgaaagcg gtgaagattg aagagagtga
    64261 aatcaactat ctgctgaaag tgtataacgc gcactttaaa aaacagctta gccgtgacga
    64321 tatcgtctgg acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca
    64381 ggctattacc cgtgattaca cccttgatat ccatgatgaa aacggcaaag caccgctgct
    64441 gtcggtattc ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg cgctggaaaa
    64501 actgacgccg tattatcagg gtattggccc ggcgtggacg aaagagagcg tgctgccggg
    64561 tggtgccatt gaaggcgacc gcgacgatta cgccgctcgc ctgcgccgcc gttacccgtt
    64621 cctgactgaa tcgctggcac gtcactacgc gcgcacttac ggcagcaaca gcgagctgct
    64681 gctcggcaat gcgggaacgg taagcgatct cggggaagat ttcggtcatg agttctacga
    64741 agcggaactg aaatacctgg tggatcacga atgggtccgc cgcaccgacg atgccctgtg
    64801 gcgtcgcaca aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg
    64861 gctggtggaa tatacgcagc agaagttgtc gctggcgtcg taaggatgat gcccggttga
    64921 agtgcaaccg ggctatttgg attccttaga ctattcattg taaataattg aataacggcc
    64981 cctgagctaa aatcgccaaa cttaagcacg ttacccaatc gccccacttc ttcctctggt
    65041 ttctattttt tctgcaccaa tggcaataat atcccaatgc aaaaccaagc tgtgcactaa
    65101 tcgggccgac aaaatcagtc caaggctgcg gccaaaaagg ggaaaaattc aggggaagcc
    65161 agacaaacgt cagaaagaaa tttcggatat tttccagcaa cgctgatttc tctggagaaa
    65221 tataaagaat gaaattcgag agaattaacc agaaatagac cggcgaaaag accagccagc
    65281 aatgcagtct gttttttaat tcaggaggcg ttctgcggga gaattgccaa ccgatgccag
    65341 ccattacact gaaaaaaagt gcatattcaa ccattaactc aagttgtagt tcctgaagag
    65401 aaatacgcac aaccaggagc ggaaaggaga atattatttc tgccagaaaa gttaacgtga
    65461 atataagtac ggctaagaca ataaccgtcg gccataccaa taatggcacg ataacaaaac
    65521 cgagaattac agggggaata taaagccgga atttaatcat ttttattttc ctggtgattc
    65581 ggatgatgcg tcatacatcg cgcataaaca ctcatcaatt atccagtcgt ctacccagac
    65641 ctgatgctgt gtgccctgtt ctggctgctt tggtgggttc tcaatcgcct gccacgcgcg
    65701 ttctttcaag atggattgat tgttgaaggc ccaggcattg atttgggtgg taatgacgtt
    65761 atcgatatcg tcctggttat ctctgatata atcctcacaa tatctgttca ataaacaggc
    65821 aagagaaacg gtcgaccaaa agccgggaac tgaattaatg gcatccagca atggcgcaag
    65881 agcgatatcg gcacggaaaa acatttccag catatactgt aattcgacag tttcatcgcc
    65941 ggatacccaa tcagtcatat actggcaggc aaatcgttgc aaaattttcc attcatcctc
    66001 acgccaattt tctttactgg caaggtttat acgccgtaga gaaaatactt cgtgaatatt
    66061 gtcaaactgc ccctgtgcaa caaattccag taaacgcggc agaaaatagc ggacctcatc
    66121 gctattttgc ggatcaggcc ctgggctact attccatgca ttgattaagg aatacgagat
    66181 cgctcgtaac gatgtactcc gtaatacctt ttgttcctgc tgactcaggc aatattcaca
    66241 acaaaccaca aatttctgcg gcaccggata acgacgaaaa atatgataaa tctgctcaat
    66301 taatgcctgc atggcggggc tgggttgcat aattaccccc tcgcccgctg atcgcgtgta
    66361 aacagttgat aaacaaaacg agcattcgcc acgttcaggc gaataatggc actacgagag
    66421 agggaaaagc tggggcgaat gcaactattg cagtctcgac agctttcttc agcgcaattt
    66481 tccactgtgt aataacgagc gtagagtttc agttccgctt gcttatcacg aaatccgcgt
    66541 aaactgttat cgttatgaaa tttccaggca tctttaaaga cctgaaaaat gtagatatcc
    66601 tcaatccggg actcacattt tttaagcggg cgctttgcta tgatcacccg ctcatccggg
    66661 tttagcatcg ccgtaaattg aacggtgagg tcatttgtcg tgttcagatc acattgaaca
    66721 tctggtacat cactgatata gtggtcaaat ccaaagtcag caccacaggc acaaagacgg
    66781 ctaatggtgg cggtatcgag ggaaaatccc ggccatacat tgctggacgt taagccaata
    66841 tttaatgtga cattatatcg aagcaatccc tcaagaccgt cagcaccgac gaacttttcc
    66901 ccttcatcca gggcctggtt aatgagttgc tgaacaatgc cttcatccca gtatgccgat
    66961 ttgcagttgg caatcgtaat atccacgcct gtgaatggat cagtaccgac aaggcttgca
    67021 aataatgtat taactaattc catttttatt atccgcctta atgcaacgtt ttatacatcg
    67081 gcacataacc tacatcttcc accaaactct gtccctgcgg cgttaaaaac cactccaaca
    67141 atttttgtgt ctctgacgtc gtattttccc gcgttaccat aaaggcatcg acgatatagg
    67201 gatatttgcc gttgcgaatg ttttcggcag tcggtgcaat accgttaatc gctagcagtt
    67261 ttatattttt atcggcgttc atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt
    67321 tgtttgtatt acggtattcg gcaacgactt taatcattcc ctccatcata cttgccactt
    67381 ctgtttcctg cggcgagatc atgcggacat ttttcatgac ctgggattgc atcaccgtct
    67441 ggctaccaga gtcttccggg cgttgccagg tctggatctc ctgatcgtta ccgccaaccg
    67501 tgcgccagtt ggtaattgca ccactgaaga tgtcacgcac ttgttgttcg gtcagggaat
    67561 taaccgggtt atctgcattg acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaaa
    67621 tcaaagtgac gcccgattcc tccgcgcgtt ttttctgccc accggaaggt tgagccacaa
    67681 agataatatc ggcattacct ttaacaatct tgttatatgc ttcgggagta cgagagttcg
    67741 ccagatattc ccattcgtga aaatcttccg gcaaaacgct tagtgcataa aaggcagagg
    67801 cataaatggg gtacgccgcc gttgcgccat caagtcgcgg ccagttttgc gtgaaatgaa
    67861 tttgcggttt accacgcagt ggtgtcagtt gattattgag tttatcgggt cgccaggccc
    67921 aggtatcaat attttcacgc acaccgacgc cgggattaag ctgtttatat aaataagcct
    67981 ggtaaccggc gacaaaacca agcatgagaa ggataaacaa gagagaatta cggcaacgta
    68041 acccggacgt gcccgttaaa ggatatctgg tcggccagcc gtaccccagc gaaaaactga
    68101 tatgcgcgcc aacaggtata atgactaacc accagtactc accaccgact aatgaggcta
    68161 acaaaacgcc cagccagggg agcataagat cagccaacca acccgctccc atctggcgaa
    68221 attgataatc tgccagatgc agcgcgatta tccagacccc cagggttaat aatgcgggta
    68281 ctaaaaaaag acattgtcgc cagacggcta tttctctttt ttctggtgca ctatcgtccg
    68341 cccacactcg ccccgagaaa aatagcatta atgcgcccag cgccagcata aaaatgttga
    68401 gcagaaggta aattgttggc gtgataagga tattacaaac ttcaataata ccggcaagtc
    68461 cgacacccag catggcaata accgccgcca aaaactgcgc cagaatgggg atgccgaaaa
    68521 aagtcattac cagcgaggtc aaaatccatt ttctgttttg cattattctt tccattcttt
    68581 ctgaatggcg aaattatact ccccgagtcc ccttgcccct tctggacact tttccgaaat
    68641 gatggcgaaa aaaacgggac cctttggccc cgttctattt attagtgaaa ttacaatctc
    68701 accggatcaa tatgccagat atgatcggcg tactctttga tagtacggtc agaagagaag
    68761 tagcccatat tggcaatgtt cagcatcgct tttgcggtcc actcttcctg aagctcgtag
    68821 agttcgtcga ctttatcctg acaatcgaca tagctgcgat aatccgccag aacctgatag
    68881 tgatcgccga agttgatcag cgaatcaacc agatcgcgat agcgacccgg atcttccgga
    68941 ctgaatacac cgctgccgat ttgcgtcagc acctgatgca gctcctcatc tttctcgtag
    69001 tattcacgcg gtttgtagcc ctgacgacgc agttcttcca cttcttccgc agtgttacca
    69061 aagataaaga tattgtcagc accgacatga tccagcatct cgacgttcgc accgtccagc
    69121 gtaccgatag tcagcgcgcc gttaagcgca aacttcatgt tactggtgcc ggaagcttcc
    69181 gtccctgcca gcgaaatctg ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg
    69241 ctaacgctgt agttcgggat gaacacgact ttcagcttat cgccaatctg cggatcgttg
    69301 ttgatcactt tcgctacgtc attgatcaaa tgaataatgt gcttcgccat gtaataggcc
    69361 gaagccgcct taccgccaaa aatattcacg cgcggtaccc acttcgcatc cgggtcggcc
    69421 ttgatgcggt tatagcgggt aatcacatgc aacacattca tcaattgacg tttgtattcg
    69481 tgaatacgtt tgatttgtac atcgaacaac gcctttggat tcaccaccac attcagctgc
    69541 tgggcgatat actctgccag acgctttttg ttctccagct tcgcctgatg cacagcgtga
    69601 ttaaccattg ggaaatcaca gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc
    69661 cagttacggc ccaggtgttc gtccagcacg gctgaaagcg atgggttcgc taccgctagc
    69721 cagcgacgcg gcgtcacacc gttggtgacg ttggtgaaac gacccgggaa gattttcgca
    69781 aagtcggcaa acaacgattg caccatcaga ttagagtgca gttccgatac accgttaact
    69841 ttgtggctca caacaaccgc cagccaggcc atacgcacac gacgaccgtt ggattcatca
    69901 atgatcgacg cccgtcccag cagatcggta tcgttcggat actgttcctg caaggttttc
    69961 aggaaatagt cgttgatttc aaagatgatc tgcaggtgac gcggcagaat tttacccagc
    70021 atatcaaccg gccaggtttc cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag
    70081 acctgacaac acacctcaaa cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga
    70141 cgcatcatct caggaatcga cagtaccgga tgggtatcat tgagatgaat cgcgatttta
    70201 tccgccaggt tatcgtaggt tttatgcaac tgataatggc ggcttaaaat gtcctgaatg
    70261 gtcgaggaaa ccaggaagta ttcctgacgc aggcgcagct cacgcccgga gtaggtggag
    70321 tcatccggat acagtacgcg agatacgttc tcggagtggt ttttatcttc cactgccgcg
    70381 aagtagtcac cctggttgaa tttaccgagg ttaatttcgc tactggcttg cgcactccac
    70441 aaacgcaacg tgttggtcgc gtcggtgtcg taaccaggga ttatctgatc gtaagcgact
    70501 cccagaatct cttcggtttc aatccagcgc gtttttttac cttcctgctg aatgcgacca
    70561 ccaaaacgga ctttatagcg cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc
    70621 agccagtagt ctggcgactc tttctggcta ccgttaacga tgttctgctt gaacataccg
    70681 tagtcatagc ggatgccgta accgcgcccc ggcaacccta acgtcgccag agaatcaagg
    70741 aagcaagccg ccagacgtcc caggccaccg ttaccgaggc ccggatcatt ttcttcatca
    70801 atcagctctt cgagatttaa ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt
    70861 cctagcgaca acatggcgtt ggagagcgta cggccaatca aaaactccat cgacaggtag
    70921 taaacctgac gagtttcttg cgacaactgg gcacggtttg aacgtaacca gcgctccacg
    70981 agacgatcgc gcacagcaaa taacgttgcg ttcagccatt catgtttatt ggcgacgacc
    71041 gggtcctttc caatcgtaaa catcagcttg taagcgatag agtgcttaag agcttctacg
    71101 ctaagcgtgg gcgatgaata tgtaaacgga gcattcatat aggcgtttcc tgaaaactat
    71161 ttcaagcgat agtaaagctc acggtacgac ttcgccgcga cctgccagct aaaatccatt
    71221 gccatagcct gacgttgcac aaaccgccac agtgaaggac gggaccacag tacaaaagca
    71281 cgccgaatag cccgtaacag cgaccaggca ttactatctt caaagacaaa cccactggcg
    71341 acgccatctg caaggttctc aagagaacag tcagaaaccg tatcagcaag cccaccggtg
    71401 cgccgcacta acggcagcgt accgtacttc aatccataaa gttgcgttaa gccgcacggt
    71461 tcaaaacggc tgggcaccag aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct
    71521 tcgtgatagc caatctgaac gcccacctga ccggggtatt ccgctgccgc cgcaaggaaa
    71581 ccttcctgca gcaccggatc gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga
    71641 agccccggta aggcttccag caccaggtcg agacctttct ggctggtcag acggctcacc
    71701 actgcaaaaa gcggcacttt atcgtcaacc ttaagcccca ttgcgatttg taactggcgc
    71761 ttattttccg ctttatcttc caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc
    71821 gtctctggac tccagatttt ctcgtccacg ccgttcagta cgcctgaaag acgcccttca
    71881 cggtgacgct gttgcaacag accttccata ccgtaggcaa actgcggttc ggtgatctcg
    71941 cgagcgtagg ttggactgac cgccgtaatg tgatcggcat agtacagacc ggccttcagg
    72001 aaagagattt gtccgttgaa ttccagccca tgaatattaa agaatgacca tggcaattgg
    72061 atgtcattca tgtgatgtgc ataaaacatg ccttgatagg ccaggttgtg cacagtaaac
    72121 accgacttcg caggacgccc gcgcgccgcc agatacgcag gcgcaagacc tgcatgccag
    72181 tcgtgcgcat gcaccacatc aggacgccag aatgggtcaa gcccgctggc catttctgcc
    72241 ccaacccacc ccagcagcgc aaaacgcaat acgttgtcgg tataggcaaa taagttggta
    72301 tcgtgatacg ggcttcccgg acgatcatag agatgcggcg cgtcaatcag gtatatgcca
    72361 accccgttgt aatgaccgaa caacagcgtg atatgcccgg cgaaagtatc acgacgggat
    72421 actacctgcg catcggtcac gccacggcga atatcgggaa atgcaggcaa cagtacgcga
    72481 gcgtcaacgc cgtctgcgat ttgtgctgcg ggtaatgccc caataacatc agccagaccg
    72541 ccggttttaa gcagcgggaa catctctgaa catacatgta aaacctgcat tatcgctcct
    72601 gtttatgccc taacttccgt agcatttcgc gcgttaccag cacgatgcct tcttctgaac
    72661 gatagaaacg acgtgcatct tcctctgcgt tttcaccaat caccatgcct tccgggataa
    72721 cacaagcacg atcgatgacg cagcggcgca gacggcacga gcgacctacc catacttccg
    72781 gtaacaatac ggcggaatca atgttgcaga atgaattcac gcgaacgcgc gagaacagaa
    72841 cggactgcac caccaccgaa ccggagatca cacaaccgcc ggaaaccagt gagttaaggg
    72901 tcatcccgtg gctaccggag cgatcctgca cgaatttcgc tggcggtaat gattcattgt
    72961 aggtgcgaat tggccaattg cgatcgtaca tatccagttc cggcaccaca gaggccagat
    73021 cgagattcgc tttccagtaa gcttccagcg tacccacatc gcgccagtac ggctcggcat
    73081 ccgggtcgga ttgtacgcaa gagagcggga acgggtgcgc ataggccaga ccggcttcgg
    73141 tgatcttggg aatcaaatct ttgccaaagt cgtggctgga gttctcatca cgatcgtctt
    73201 cttccagcag ttcatacaga tagtcggcgt caaagacgta gatacccata ctcgccagag
    73261 atttgctcgg atcgttcggc attgacggtg ggttagcagg tttttcaacg aattcgataa
    73321 ttttatcgtt ctcatcaacc gccataacgc caaatgcgga ggcttcttca atcggtactg
    73381 gcatacaagc aacggtgcaa cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt
    73441 agtcttgctt gtagatatgg tcgcccgcca ggatcaccac gtattccgct ttataacggc
    73501 ggataatgtc gaggttttgg gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt
    73561 tcattctctg ctgtgctggc agcagatcga caaactcgtt catttcttca ttgaagaatg
    73621 accagccgcg ctgaatgtgc tgcaccagag tgtgggactg gtactgggtg atcacgccca
    73681 tacgacggat cccagagttg atgcagttag acagcgcaaa gtctataatg cggaacttac
    73741 cgccgaagtg tacggccggt tttgctcgct tattggttaa atccttcagg cgggtaccac
    73801 gtcctcccgc cagtatcagg gcaacagatt tcaatggcag ctggcgcgcc aacattaagt
    73861 gatcgttctt ctctaaacta accatgacta actccttttt tatcatctct ggaacacaca
    73921 caatccgtgt gcaggtccct gccagacagc cgtaatcact gggttatcct ctccagcgaa
    73981 tgggggaatg gcgtgccact ccccagcagg taaaacaatc tctgttacct caagcgtggc
    74041 gttaattgcg atcaaaaagc gatccgagag cagaatttgc agctgtttcg gcccgttttg
    74101 ccactcatcc gtgcttaaag gttgagcata tcgatttagc caacggacat tgccgtcgcc
    74161 ttcttcccac cagcgattct ccaccaaagc ggaaatacgc ttgcgcagat ggattaacgc
    74221 ggcggtaaat gcggttaaac cactgcttgc ctgcgaccag tccaaccagg ttaattggtt
    74281 atcctgacag taggcattgt tattgccatg ctggctgtga ccatgttcgt caccggccag
    74341 taacatcggc gtgccctggg agagcaacaa cgttgttaac agggcatgaa tgctgtcgcg
    74401 ccgccgttca accaggtcaa gagaaccgcc taacccttct ttaccatgat tgttactgta
    74461 attgttgttg gtcccgtcgc gattttcttc tccgtttgct tcattgtgtt tatggttgaa
    74521 gcaaacgcag tcgcgaagcg taaaaccgtc atgtgcggtg acgagattaa tcgcggcact
    74581 cggcagacga ccattacgtt taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc
    74641 ccccagaggc aaatcataat gcagccagaa acgacgggca gcatcgcgga aatgatcgtt
    74701 ccactcggca aacagcggcg ggaaatttcc cacctgataa ccaccaggag cgatatccca
    74761 cggttcagca attaacttca cctgcgagag caccgggcag ttctggatag cggtaaacaa
    74821 cggcgcatcc tgacggaact ctggcgtacg gcccatgact gccgccagat caaagcggaa
    74881 accatcgacg tgacaggttt ctacccaata acgcaggcag gcgcaggcat aatccaccac
    74941 cgccggatga ctcaaattga gcgtgttgcc acaaccggtc cagttgtgat aatcgccgtc
    75001 ttctcttatc caataatagc tacggttgtc gatcccacgc agcgagaata acgggccgtc
    75061 gaggtccagt tccgcactat ggttgagcac gatatcaaga atgacttcga tacccgcttt
    75121 atgcagtgct ttgattgcat cgcgaaactc atgcagcgcc gtttctggcg agcaggcata
    75181 cgccggatgc agcgcaaaca tcgccaccgg gttgtaaccc cagtagttac ttagccccat
    75241 gcgttgcaga cgtggttcac tggcaaactg cgccactggc agcagttcca gcgcggtaat
    75301 gcccaattgt ttcaaatagt tgatcatcac cggatgtccc agggctttat aagtgccacg
    75361 gatctcgacc gggatctccg ggtgcaggta cgttaatcct ttaacatggg cttcataaat
    75421 gatggtgctg ccccacggcg tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac
    75481 caccactacg catttcggcg caatggcggc gttgtcgcga tagtcaggtt cattatgacc
    75541 ggcgtgcagc agcgggttat ctttgaattc cccgtcaatt tgccgcgcgc aaggatcaat
    75601 caacaacttc gccgggttaa agcgatgccc ctcggcgggt tgccaggggc catgaacgcg
    75661 ataaccataa cgcaaacccg ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact
    75721 gtgccctggc aagtcatagc gatgttcctg gccattggcg tcaaagacac acagctctac
    75781 ccgctcggca tgagcggaga aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc
    75841 gaggggagcg ggtttgccaa tggcgagtcg tgtcattctg cctcccgaac cagccagata
    75901 gtggccagcg gtggtagtgt caggcttagt gaatgctgac gaccgtggct ggcaatctca
    75961 tcgctgtgta ccgtgccgcc attgcctgca ttactgccgt gatagtgcat ggaatcggta
    76021 ttgaggattt cacgccattt gcctggctga ttaatgccga agcgataatc atgacgcggc
    76081 accggcgtaa agttactggc aacgataatt tcgttaccct ctttatcgcg acgcacaaag
    76141 atcagcaccg agcgttcttt gtcatccacc accagccatt caaagccgta cgggtcaaaa
    76201 tccagttcat gcattgcttt atggtggcgg taggtgaggt tcagatcgcg caccagacgc
    76261 tggacaccgt ggtgccagtt atcgccgcct tccaacagat gccagtcgag gctggcatca
    76321 tggttccact cacggccttg ggcaaattcg ttccccatga acagcagttt cttgcccggg
    76381 aatgcccaca tccagccata gtaggcgcgc aggttagcga atttctgcca tgcgtcgccc
    76441 ggcatgcggt cgagaatcga ttttttaccg tggaccactt catcatgcga caacggcagg
    76501 acgaagtttt cagtgtagtt gtagagaatc ccgaaggtca gtttatcgtg atgatactga
    76561 cgataaaccg ggtcgagctt catgtagtcc agggtgtcat gcatccagcc gaggttccac
    76621 ttgtaccaga agcccagacc gcccatatcc tgcggacgag aaacgccagg gaaatcggta
    76681 gactcctcag ccattgtcac cgcaccggaa acctgctcac caagaatacg gttggtatta
    76741 cgcaagaatt caatcgcttc aagattctcg cgcccgccaa attcgttcgg gatccactcc
    76801 ccctctttac ggctgtagtc gcgataaatc attgacgcca ccgcatcgac gcgcagcgca
    76861 tcaataccaa aacgttcaat ccagtaaagc gcgttaccga cgaggaagtt actgacttca
    76921 cggcgaccat agttgtagat cagcgtgttc cagtcctgat gatagccttc acgcggatcg
    76981 ctgtgttcat acaagttcgt gccatcaaat tcggcaagcg caaagtcatc agtcgggaag
    77041 tggcctggca cccagtcgag aatcacgttc agaccagctg cgtgtgcggc atcaatgaaa
    77101 taacggaagt cgtcgcgagt accaaaacgg cgggttggcg catacaggcc ggttggctga
    77161 taaccccaac tgccatcgaa gggatgctca ttaatgggca gtagttcgag gtgggtaaag
    77221 cccatccatt tagcataagg caccagttga tcggccagct cgcggtagct caaccagaaa
    77281 ttgttgtcgg tgtggcgacg ccaggaaccc aggtgaactt cataaataga gattggcgca
    77341 tcaaactgat tcgctttttt gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa
    77401 ataagagacg cggtttccgg gcgcatttgc gcttcgaagg cataagggtc ggacttcaga
    77461 cgcaagttgc cattggcatc aatcatctcg tatttataga gctgaccgtt atgcgcccca
    77521 gggataaaca gttcccagat gccgctctct ttacgcaggc gcatcgggtg acggcgaccg
    77581 tcccagtagt tgaattgccc aaccaccgag acccgacggg cgtttggagc ccagacagag
    77641 aaacgcgtac ctgtgacgcc atccatagta tctgcatgcg cgcctaaggt ttcatacggg
    77701 cgcaggtgag taccttcaga taatagccag gcatccattt cctggattag cggaccaaaa
    77761 cggtaaggat catcaatcag gttttgctga ccatgccaga caacagccaa ctgatagcgg
    77821 aaaaaattct tacgtcgcgg aatgacgccg ctaaagaatc cccgtgagtc gagacactcc
    77881 agttttgcga gtttgcgccc ggttttcggt tcaatcaccc acacatcggt agcgtcgggt
    77941 aaaagggcac ggacttccag tcccgcggtg gttttatgca ttcccagtac ggaaaaagga
    78001 tccgcaaaat ggcctgcaat tagcgcgtta atcacgtctc tatcgatacg atcggacatg
    78061 cttgtcttcc tgttttattg tgtcacccca tccaattgga tttttgactt ctggttgtga
    78121 catttttttg acctgaacgg cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc
    78181 aggtaaggct gtgaatactc atgtattcag ccacccttaa agaatagcca atgctctatt
    78241 taactcccgg taaatcatga aacatctgcg cttactcctg tattacgcac taacaggggc
    78301 ggcatcgcgc cccagattta atgaataaag attacgccag ttgacgaagc atccgacgca
    78361 gcggctccgc ggccccccac agcagctggt cgcccacggt aaaggctgac aggaactctg
    78421 gtcccatatt cagcttacgc aggcggccta ccggcgtggt cagcgtgccg gtaacggcag
    78481 ctggggttag ctcacgcata gtgatttccc gatcgttcgg aacgactttc gcccacggat
    78541 tgtgcgcagc cagcagttct tccacggtcg gaatagacac atcttttttc aatttaatag
    78601 tgaatgcctg gctgtggcag cgcaatgccc cgacacgcac acataaacca tctaccggaa
    78661 ttacggaaga tgtgttgagg atcttgttgg tttccgcctg ccctttccac tcttcgcggc
    78721 tctgaccgtt atcgagctgt ttgtcgatcc acggaatcag gctacccgcc agcggcacgc
    78781 caaagttatc caccggcagc tcaccgctac gggttaaggt tgtgactttg cgttcgatat
    78841 cgagaatagc agaggacggg gtcgcgagtt catctgccac atggccatac agatggccca
    78901 tctgggttaa taactcacgc atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg
    78961 caacggacac ccaatcaaca agatcattgg cgaataaacc acccaacgac atcaacatca
    79021 ggcttacggt acagttaccg ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa
    79081 tgacgtcctg attgacgggg tcaagaatga tgatggcgtc atctttcatg cgcagagacg
    79141 atgctgcgtc aatccagtaa ccttgccatc cgctttcacg aagctttgga tagatttcgt
    79201 tggtataatc gccgccctga caggtcacaa tgatatcgag ggcctttagc gcctccagat
    79261 caaaggcatc ctgaagtgtg ccagtggttc cgccaaaaga cggcgcagcc tggccaagct
    79321 gagaagtaga aaagaagaca gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt
    79381 gcatgagaac ggagccgacc ataccgcgcc agccgataaa accaacattt ttcataagcg
    79441 tttttttcct gcaaagatgt gtgctgtata aatgtgccgg tctcctcttg gcacatcttt
    79501 caccatacaa aaagcagcca aagtcgcaag tgaaattaat caatgatagc gaagccatca
    79561 gtaatgcgac ttatcctgct ttgttagcac gcagaaagtc cgcggcaatt atcagggaat
    79621 ttgagttatg aatgaaatca tttctgcagc agttttattg atcctgatta tggatccgct
    79681 cggaaaccta cctattttca tgtccgtact gaaacatact gaaccgaaaa gacggcgggc
    79741 aatcatggtg cgagagttgc ttattgctct cctggtgatg ctggtgttcc tgtttgcggg
    79801 tgagaaaatt ctggcatttc ttagcctacg agcagaaacc gtctccattt ctggcggcat
    79861 cattctgttt ctgatcgcca ttaaaatgat tttccccagc gcttcaggaa atagcagcgg
    79921 gcttccggca ggtgaagagc catttatcgt gccgttggca attccgttag tcgccgggcc
    79981 gactattctc gccacgctga tgttgttgtc tcatcagtac ccgaatcaga tggggcatct
    80041 ggtgattgct ctgctgctgg cctggggcgg cacctttgtc atcctgctac agtcttcgct
    80101 atttttacgt ctgctgggcg agaaaggggt gaacgcactt gaacgcctga tgggattgat
    80161 tctggtgatg atggcaaccc agatgttcct cgacggcatt cgaatgtgga tgaaggggta
    80221 acgaggagag tttttcgaaa agaacccggt agcgtcacaa ctaccgggcg aacaaactta
    80281 actcaacagc tggaacgcaa tcatcccaac gatggcaccg acagtgccga ggatggtttc
    80341 catcatggtc caggttttca gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa
    80401 ccagaaacca gcgtcgttaa cgtggctgac aacaatcgaa ccaccagcga tacaaatcga
    80461 cagtgccgcc atttgcgcac cagagtagtt cagttgttca atgactggca tcaccagtcc
    80521 caccgccgtt aaacaggcta cggtggcaga cccctgaatg atgcgcactg cagcggccag
    80581 cacgaagcag gtgatagcaa tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg
    80641 acctacgcca gagtcaacca gcacctgttt gaacacgccg cctgcaccaa tcaccagcag
    80701 aatgatcccc gccggttgta gcgcgtgacc gcaaatctcc atcactttgt cttttggcat
    80761 gccctgacgc attgccaggc cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa
    80821 tggatgacca ataaactcga accattcgta agcagttgat ccttctggca caaaacgagc
    80881 ggcaatggtt ttcagcccca ccagcaccag cggcaacagg atcagcgaca ggctgaatcc
    80941 gaaggacggc attttgcctt cgccgagatg cggttcgctg atgtcgtcag gaatatgcaa
    81001 ctccacgtaa cggctgatga aattacccca cagcggcccg gcaataatca ttcccggaat
    81061 tgccgcacac agaccaatca ggatcatcca gccaaagtcg gcattcatct gcgatgcgag
    81121 gagcattggc gctggcccag gcaccaggaa cgcagccgcg gccgccacgc ctgcgaataa
    81181 tgggattacc agcttcacca ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct
    81241 aatcagcaga acaatcgcca cttcaaagaa cagcggtagc gcacagacca gccccgcaag
    81301 gccgatggca taatgcgcgc ggctgtgacc gaaggatttg agcattttga cggcaatctg
    81361 atcgactgcg ccggtttcat gtaagatctt gccaaacata gctcccaggg cgacaaccac
    81421 cgccaggaag ccgagggtgc ctcccatccc tttttccatc gtcgctgcga ttttatcgag
    81481 cggcataccg gaaaaaaggc cagcccccat ggacaccacc atcaaagcca ggaaagcgtg
    81541 catacgcgcc ttcatgacta aaaacagcag cagtaaaaca gaccctactg ctgttaaaac
    81601 aagcgttaat gtagtcacta cttatttgcc ttttttaata acctcaatgg tgcttgccac
    81661 aacaccttcc agcggttgat cgatatccac caccagtaca tcagtttcgt ccgcacccgg
    81721 ctcctgcagc gtttcaaact gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg
    81781 cgctttcagg cggctttcaa tcacgtcaaa atcgcctttc aggtagatga aagagagatt
    81841 cggattacct tcacgcagca agtcgcgata gtgttttttc aatgcagaac agacgatcag
    81901 tgacacttta ttagtgcgct gcatagcaaa cgcggcgtcg ttcagcgcct gcaaccaggg
    81961 tttgcgatcg tcgtcattca gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg
    82021 atggaggaaa tcgccatcaa gaaacgcggc atgaagttga tgcgccactt cactggcgac
    82081 cgcagatttg ccgctgcccg atacgcccat caagacgtaa atgtggtgat catggttagt
    82141 cgtgctcaaa gtggtgcccc cacaatacaa gaattaaaaa tgttacgggt aactgttatc
    82201 ggtaacattg tccagccgga caatatcaga agcaatatcc atccgtgcct taagtgtata
    82261 agtgtgagct acttcaaatt tgtaggctta aatagatccg cccggtgaca aggtgaaacc
    82321 taaatctaac attttcggtg tcacagattc gccacgaata cgcgccagca ggcgttcagc
    82381 gccaatactg cccatccgct cacgcggcgt cagtacgctc gccaaccgtg gttccatcac
    82441 ctgaccaatg tcatgaccgt ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc
    82501 cagacgctgg cattcaaacg ccgcgccgac cgccaggtca tcattagtac agaacacgcc
    82561 atccagctgc ggatattccc gcctcgcctg gcgaatcagt tcaataccgg aagagtaaga
    82621 agaagattgc tcaaccatca cgctatatgg caccaggtct gcatccagca tcgcctgttc
    82681 gtatcccttc tgtttgatga tagtacgttc gtcgagacgt gcgccgagat aagcaatgtg
    82741 gcgatgcccg cgagcaataa tggcggtggt catctggcgt gccgcttcaa agttatcaaa
    82801 accgacggca atatcgaggc acggcgactt gctgtccatc aactccacca ccgggatccc
    82861 cgccacttca atcatcttta aggtgcgtgg cgtgtgagta cgttcggtga ggatcagtcc
    82921 atcgatattc catgagagca tggattcgag gcgttcttgc tccatttccg gtttataacc
    82981 gtagtgcgcc agcatggttt gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa
    83041 tacttccgcg aaaacctggt tggtgagaga aggtaacagg acgccaatcg cccggctggt
    83101 ggcgttagag aggatatcgg gcgcacgatt gggaatataa cccagttcat caagagcggc
    83161 ggcaatcttg ccgcgtagag cgacggaaac ctgctccggg ttacgcaaaa agcggctgac
    83221 cgtcattttg gtcacgccta cacggtcagc cacatcctga agtacgggtc ttttcttttt
    83281 catcgtcctg aaggtacaaa agagatagat tctcttagtt taacacggac gcatcagaac
    83341 cttcccgggg aaaacagtgc ttgtttacaa ttattagata tacactaaat caattgcatc
    83401 gcattgtgct aacgacgcgg caaaaccccg gaagcgtgca tcagcacgcg accggagcga
    83461 gcgaggaagc ctgcaaaaag gcaggcttca aagatgccgt tgagttttaa accggcggca
    83521 gatcgaacag taacacttcg ctgtcgctat ccgcatggat ggagattgcc tgctcatccc
    83581 agattgccag accatcgctg gtcgaggctt tcacgccgtt aatggtgaca ttgcctttca
    83641 ccacctggat ccagacgcgg cgttcagcgg caatctgatg caccgactgc tcatctttca
    83701 gcaacgccca acgatacagt tccatatcct gatgcacttt caatgaacca tctcgagcat
    83761 ccggcgagag caccagctgt ttgccctgta cggcatcgaa gcgacgctgt tcataacgcg
    83821 gcgtaatgcc attttcttca ggcatgatcc agatctgata cagatgcaga cgctcggtgc
    83881 tgcttgggtt gtactctgag tgacgaatac ccgtacccgc actcataatc tggaactcac
    83941 ccgccggaac ctgctcttta ttgcccatgc tgtcctgatg ctcaacagta ccttccagca
    84001 cgtaggtcaa aatttccata tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa
    84061 tcacgtcgtc gttaatcacg cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt
    84121 tggcaaaaga gaaagtatgc caggagtcca gccagccatg atttgcatga ccacgttcat
    84181 ttgctttgcg taagtagatc atttgtattc gccccatgaa tgatttcgat gggcttagtg
    84241 tggacccgat ctgcctggga tgatagaggg tgaaaattga cccctctgtt caaaaaaatt
    84301 gaactattcg aggggccatc aaacttactt cgcgagagtc actgtggagg gagaggcttg
    84361 ctcaaatccg cgttcaagga tttccagatt ggtaagaact tcagattcct tgacgtaatt
    84421 tggcgcaccg tgggtgatgg tttgatacaa cgcatcataa acgcgcccgt aatcgcccat
    84481 ctccggcttc atctcttctc tgaccgtcac gccctcgtca ttgacatact ccagcacacc
    84541 gaccgaatca tccgctgcga atcccggttc gcccggcata atattagcct tcaggctggt
    84601 ttcctgctgg tcgataccgt atttaataaa cgaacctttc ttaccgtgaa cgataaattt
    84661 cggataatcg attttcacca gatggctggt tttgacgatg gctttcaggt cgccataaaa
    84721 cagttgcgct tcaaaggtgt cgtcaggatt ggctttatta cgcaggctgc ggatgtcata
    84781 agcgacgtga tccgggcgac cgaacagaga aataatctgg tccatcgtat gcacaccaag
    84841 gccataaaac gcgccatcct gcggcagccc aggtttggtt tctgccaccg ggcggtaata
    84901 gtcaaaatgg ctttccactt caacaatctc tcccaacttg ccactttcaa tcgctttttt
    84961 cgctgtcaga aagcaggagt caaagcgacg attctgatac ggcgtgacgg tcagcccttt
    85021 gcttttcgcc aacgcaaaca gctccttcgc ctgggcaagc gtcggggtga acggtttttc
    85081 gaccagcaca tttttcccgg cttccagcgc gcgtttcgcg tactcgaaat ggctgtccgc
    85141 gtgggtgcag acaacaacca gcttaacatc gggatcgttt agtacttcgt cgagatcgct
    85201 ggtgaaatgg atatgggaat aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa
    85261 aatatgcgcg acatgccagc tatccttgcg gttaagtaca tacggcagat ggtaacgggt
    85321 ggtgcttttg ccgaagccaa taaaggcgca gttgatgacc atgatttcgt cctttttaag
    85381 gtggttattt acaccttagc gcaaagcgga cgtggttcct actggagtgc gcataacgaa
    85441 cacaagcact cccgtggata aattgagaac gaaagatcaa aaaaaaagcc agcacccggc
    85501 tggctaagta atactggaag caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg
    85561 tcttcctgat cgcgagacaa taataatcat tctcattcgc acttgtccaa cactttttgc
    85621 aaaaaaatgc atttgactcg catttgaaag tcaatgatgt tgaaagggac atttacccca
    85681 aagaggacaa aggaatgagt gagatagtaa tacgccacgc agaaacacgg gattacgagg
    85741 ccatcaggca gattcacgcc cagccggagg tgtattgcaa cacactacag gtgcctcatc
    85801 cttccgatca tatgtggcag gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg
    85861 cctgtattga tggagacgtc gtgggccatc tcaccattga cgtgcaacag cgcccacgcc
    85921 gcagtcatgt tgccgatttt ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg
    85981 ccagcgccct gatgcgagag atgattgaaa tgtgcgacaa ctggttgcgg gtagatcgca
    86041 ttgaactaac cgtgtttgtc gataacgccc cggcaattaa ggtctataaa aaatacggct
    86101 ttgaaattga agggactggt aagaagtacg cattgcgtaa tggtgaatat gtcgatgcat
    86161 attatatggc gcgggtgaag taagatagtg ccctttttct gagatggaaa aagggtgtca
    86221 ttcaaaatcg acataccttc ctttaaggta tttatttgcc caatacatat attgagattt
    86281 aactcataat tacttccta
//
LOCUS       DAOFQD010000003        80457 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.003, whole genome shotgun sequence.
ACCESSION   DAOFQD010000003 DAOFQD010000000
VERSION     DAOFQD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.003"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            44..610
                     /locus_tag="QBF46_000171"
     CDS             44..610
                     /locus_tag="QBF46_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6672415.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANKINESP"
     gene            753..1925
                     /locus_tag="QBF46_000172"
     CDS             753..1925
                     /locus_tag="QBF46_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDQ6672416.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTIVVRVPQGETEDETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQKDVTTLVRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQVSA"
     gene            1935..2450
                     /locus_tag="QBF46_000173"
     CDS             1935..2450
                     /locus_tag="QBF46_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDQ6672417.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            2505..2807
                     /locus_tag="QBF46_000174"
     CDS             2505..2807
                     /locus_tag="QBF46_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDQ6672418.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            2822..2941
                     /locus_tag="QBF46_000175"
     CDS             2822..2941
                     /locus_tag="QBF46_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDQ6672419.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            2934..6011
                     /locus_tag="QBF46_000176"
     CDS             2934..6011
                     /locus_tag="QBF46_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDQ6672420.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISAKDDAGATARLARETSRYNQELSKQEAR
                     LKRLGEAQRRMNAARASYARSLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLQV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKASAVVAAVT
                     VGLGTLAVAMAAVLGPLAVIRLGFSVLGIKTLPSVTAAVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAAAGTVLRVLASGPLAMLRVALYAISGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPISAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPAKGGEAIVSRAATVPLV
                     QRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            6008..6493
                     /locus_tag="QBF46_000177"
     CDS             6008..6493
                     /locus_tag="QBF46_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6672421.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PENDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTATSALSDISKTVGGLL
                     S"
     gene            6490..7590
                     /locus_tag="QBF46_000178"
     CDS             6490..7590
                     /locus_tag="QBF46_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDQ6672422.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMSLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVISITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVKIDDFEME"
     gene            7681..7899
                     /locus_tag="QBF46_000179"
     CDS             7681..7899
                     /locus_tag="QBF46_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDQ6672423.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(8135..9820)
                     /locus_tag="QBF46_000180"
     CDS             complement(8135..9820)
                     /locus_tag="QBF46_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDQ6672424.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            10090..10467
                     /locus_tag="QBF46_000181"
     CDS             10090..10467
                     /locus_tag="QBF46_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDQ6672425.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(10497..10754)
                     /gene="grxA"
                     /locus_tag="QBF46_000182"
     CDS             complement(10497..10754)
                     /gene="grxA"
                     /locus_tag="QBF46_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDQ6672426.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNEHDDFQYHYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10914..11201
                     /locus_tag="QBF46_000183"
     CDS             10914..11201
                     /locus_tag="QBF46_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDQ6672427.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLSPQLMNRPPQYSRSEREKDNDANH"
     gene            11185..11906
                     /gene="nfsA"
                     /locus_tag="QBF46_000184"
                     /pseudo
     CDS             11185..11906
                     /gene="nfsA"
                     /locus_tag="QBF46_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            11967..12869
                     /gene="rimK"
                     /locus_tag="QBF46_000185"
     CDS             11967..12869
                     /gene="rimK"
                     /locus_tag="QBF46_000185"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDQ6672428.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12957..13433
                     /locus_tag="QBF46_000186"
     CDS             12957..13433
                     /locus_tag="QBF46_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDQ6672429.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            13784..14896
                     /gene="potF"
                     /locus_tag="QBF46_000187"
     CDS             13784..14896
                     /gene="potF"
                     /locus_tag="QBF46_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDQ6672430.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            14991..16124
                     /gene="potG"
                     /locus_tag="QBF46_000188"
     CDS             14991..16124
                     /gene="potG"
                     /locus_tag="QBF46_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDQ6672431.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            16134..17087
                     /gene="potH"
                     /locus_tag="QBF46_000189"
     CDS             16134..17087
                     /gene="potH"
                     /locus_tag="QBF46_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDQ6672432.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            17084..17929
                     /gene="potI"
                     /locus_tag="QBF46_000190"
     CDS             17084..17929
                     /gene="potI"
                     /locus_tag="QBF46_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDQ6672433.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            17989..18477
                     /locus_tag="QBF46_000191"
     CDS             17989..18477
                     /locus_tag="QBF46_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDQ6672434.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            18518..19645
                     /gene="rlmC"
                     /locus_tag="QBF46_000192"
     CDS             18518..19645
                     /gene="rlmC"
                     /locus_tag="QBF46_000192"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDQ6672435.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWVRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDVRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(19844..20575)
                     /gene="artJ"
                     /locus_tag="QBF46_000193"
     CDS             complement(19844..20575)
                     /gene="artJ"
                     /locus_tag="QBF46_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDQ6672436.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(20801..21469)
                     /gene="artM"
                     /locus_tag="QBF46_000194"
     CDS             complement(20801..21469)
                     /gene="artM"
                     /locus_tag="QBF46_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDQ6672437.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(21469..22185)
                     /gene="artQ"
                     /locus_tag="QBF46_000195"
     CDS             complement(21469..22185)
                     /gene="artQ"
                     /locus_tag="QBF46_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDQ6672438.1"
                     /translation="MNEFFPLASAAGMTVDLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(22192..22923)
                     /gene="artJ"
                     /locus_tag="QBF46_000196"
     CDS             complement(22192..22923)
                     /gene="artJ"
                     /locus_tag="QBF46_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6672439.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(22941..23669)
                     /gene="artP"
                     /locus_tag="QBF46_000197"
     CDS             complement(22941..23669)
                     /gene="artP"
                     /locus_tag="QBF46_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDQ6672440.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(23887..24402)
                     /locus_tag="QBF46_000198"
     CDS             complement(23887..24402)
                     /locus_tag="QBF46_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6672441.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            24528..24851
                     /locus_tag="QBF46_000199"
     CDS             24528..24851
                     /locus_tag="QBF46_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDQ6672442.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            24848..25678
                     /gene="amiD"
                     /locus_tag="QBF46_000200"
     CDS             24848..25678
                     /gene="amiD"
                     /locus_tag="QBF46_000200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDQ6672443.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTTSL
                     LELLARYGYDVKPDMIPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(25675..26688)
                     /locus_tag="QBF46_000201"
     CDS             complement(25675..26688)
                     /locus_tag="QBF46_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDQ6672444.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(26787..28217)
                     /locus_tag="QBF46_000202"
     CDS             complement(26787..28217)
                     /locus_tag="QBF46_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDQ6672445.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28228..29229)
                     /gene="ltaE"
                     /locus_tag="QBF46_000203"
     CDS             complement(28228..29229)
                     /gene="ltaE"
                     /locus_tag="QBF46_000203"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDQ6672446.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKHAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29266..30984)
                     /gene="poxB"
                     /locus_tag="QBF46_000204"
     CDS             complement(29266..30984)
                     /gene="poxB"
                     /locus_tag="QBF46_000204"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDQ6672447.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31117..32085)
                     /gene="hcr"
                     /locus_tag="QBF46_000205"
     CDS             complement(31117..32085)
                     /gene="hcr"
                     /locus_tag="QBF46_000205"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDQ6672448.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32097..33749)
                     /gene="hcp"
                     /locus_tag="QBF46_000206"
                     /gene_synonym="priS"
     CDS             complement(32097..33749)
                     /gene="hcp"
                     /locus_tag="QBF46_000206"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDQ6672449.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(33893..34792)
                     /gene="lysO"
                     /locus_tag="QBF46_000207"
     CDS             complement(33893..34792)
                     /gene="lysO"
                     /locus_tag="QBF46_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDQ6672450.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(35287..35982)
                     /gene="aqpZ"
                     /locus_tag="QBF46_000208"
     CDS             complement(35287..35982)
                     /gene="aqpZ"
                     /locus_tag="QBF46_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDQ6672451.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            36408..38066
                     /locus_tag="QBF46_000209"
     CDS             36408..38066
                     /locus_tag="QBF46_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDQ6672452.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38063..39019)
                     /locus_tag="QBF46_000210"
     CDS             complement(38063..39019)
                     /locus_tag="QBF46_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDQ6672453.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFAKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39170..40285
                     /gene="macA"
                     /locus_tag="QBF46_000211"
     CDS             39170..40285
                     /gene="macA"
                     /locus_tag="QBF46_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDQ6672454.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            40282..42228
                     /gene="macB"
                     /locus_tag="QBF46_000212"
     CDS             40282..42228
                     /gene="macB"
                     /locus_tag="QBF46_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDQ6672455.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(42301..42525)
                     /gene="cspD"
                     /locus_tag="QBF46_000213"
     CDS             complement(42301..42525)
                     /gene="cspD"
                     /locus_tag="QBF46_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDQ6672456.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            42848..43168
                     /gene="clpS"
                     /locus_tag="QBF46_000214"
     CDS             42848..43168
                     /gene="clpS"
                     /locus_tag="QBF46_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDQ6672457.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            43199..45475
                     /gene="clpA"
                     /locus_tag="QBF46_000215"
     CDS             43199..45475
                     /gene="clpA"
                     /locus_tag="QBF46_000215"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDQ6672458.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46059..46146)
                     /locus_tag="QBF46_000216"
     tRNA            complement(46059..46146)
                     /locus_tag="QBF46_000216"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46110..46112),aa:Ser,seq:gga)
     gene            complement(46400..46618)
                     /gene="infA"
                     /locus_tag="QBF46_000217"
     CDS             complement(46400..46618)
                     /gene="infA"
                     /locus_tag="QBF46_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDQ6672459.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(46903..47607)
                     /gene="aat"
                     /locus_tag="QBF46_000218"
     CDS             complement(46903..47607)
                     /gene="aat"
                     /locus_tag="QBF46_000218"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDQ6672460.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(47649..49370)
                     /gene="cydC"
                     /locus_tag="QBF46_000219"
     CDS             complement(47649..49370)
                     /gene="cydC"
                     /locus_tag="QBF46_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDQ6672461.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(49371..51137)
                     /gene="cydD"
                     /locus_tag="QBF46_000220"
     CDS             complement(49371..51137)
                     /gene="cydD"
                     /locus_tag="QBF46_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDQ6672462.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51260..52225)
                     /gene="trxB"
                     /locus_tag="QBF46_000221"
     CDS             complement(51260..52225)
                     /gene="trxB"
                     /locus_tag="QBF46_000221"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDQ6672463.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHDHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            52769..53263
                     /gene="lrp"
                     /locus_tag="QBF46_000222"
     CDS             52769..53263
                     /gene="lrp"
                     /locus_tag="QBF46_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDQ6672464.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            53398..57465
                     /gene="ftsK"
                     /locus_tag="QBF46_000223"
     CDS             53398..57465
                     /gene="ftsK"
                     /locus_tag="QBF46_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDQ6672465.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPFLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVETAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQPQYQQPQQPV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            57620..58231
                     /gene="lolA"
                     /locus_tag="QBF46_000224"
     CDS             57620..58231
                     /gene="lolA"
                     /locus_tag="QBF46_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDQ6672466.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58242..59585
                     /gene="rarA"
                     /locus_tag="QBF46_000225"
                     /gene_synonym="mgsA"
     CDS             58242..59585
                     /gene="rarA"
                     /locus_tag="QBF46_000225"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDQ6672467.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDRGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            59676..60968
                     /gene="serS"
                     /locus_tag="QBF46_000226"
     CDS             59676..60968
                     /gene="serS"
                     /locus_tag="QBF46_000226"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDQ6672468.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61207..63651
                     /gene="dmsA"
                     /locus_tag="QBF46_000227"
     CDS             61207..63651
                     /gene="dmsA"
                     /locus_tag="QBF46_000227"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDQ6672469.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYGIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            63662..64281
                     /gene="dmsB"
                     /locus_tag="QBF46_000228"
                     /pseudo
     CDS             63662..64281
                     /gene="dmsB"
                     /locus_tag="QBF46_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
     gene            64283..65146
                     /gene="dmsC"
                     /locus_tag="QBF46_000229"
     CDS             64283..65146
                     /gene="dmsC"
                     /locus_tag="QBF46_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDQ6672470.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65181..65807)
                     /locus_tag="QBF46_000230"
     CDS             complement(65181..65807)
                     /locus_tag="QBF46_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDQ6672471.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66121..67269
                     /locus_tag="QBF46_000231"
     CDS             66121..67269
                     /locus_tag="QBF46_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6672472.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLAGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            67479..68909
                     /locus_tag="QBF46_000232"
     CDS             67479..68909
                     /locus_tag="QBF46_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6672473.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLMLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(68910..69818)
                     /locus_tag="QBF46_000233"
     CDS             complement(68910..69818)
                     /locus_tag="QBF46_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6672474.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            69918..70508
                     /locus_tag="QBF46_000234"
     CDS             69918..70508
                     /locus_tag="QBF46_000234"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HDQ6672475.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTLEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLINMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(70590..71330)
                     /gene="pflA"
                     /locus_tag="QBF46_000235"
     CDS             complement(70590..71330)
                     /gene="pflA"
                     /locus_tag="QBF46_000235"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDQ6672476.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(71522..73804)
                     /gene="pflB"
                     /locus_tag="QBF46_000236"
     CDS             complement(71522..73804)
                     /gene="pflB"
                     /locus_tag="QBF46_000236"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDQ6672477.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(73859..74716)
                     /gene="focA"
                     /locus_tag="QBF46_000237"
     CDS             complement(73859..74716)
                     /gene="focA"
                     /locus_tag="QBF46_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDQ6672478.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(75122..76882)
                     /locus_tag="QBF46_000238"
     CDS             complement(75122..76882)
                     /locus_tag="QBF46_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDQ6672479.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPSSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            77011..77703
                     /locus_tag="QBF46_000239"
     CDS             77011..77703
                     /locus_tag="QBF46_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDQ6672480.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            77902..78990
                     /gene="serC"
                     /locus_tag="QBF46_000240"
     CDS             77902..78990
                     /gene="serC"
                     /locus_tag="QBF46_000240"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDQ6672481.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            79061..80344
                     /gene="aroA"
                     /locus_tag="QBF46_000241"
     CDS             79061..80344
                     /gene="aroA"
                     /locus_tag="QBF46_000241"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDQ6672482.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT        19691 a        19921 c        21523 g        19322 t
ORIGIN      
        1 agctaactgg catcatctcc ggtttttatt cagggggatg atcatgctta ttggctatgt
       61 acgcgtgtca acaaatgacc agaacaccga tttgcaacgt aatgcactga actgcgcggg
      121 atgtgagcgg atttttgagg acaaaatcag tggcactaag tccgacagac cggggctgaa
      181 aaaactactc aggacactat cggcaggaga cacgctggtt gtctggaagc tggacaggtt
      241 ggggcgcagt atgcggcatc ttgttacgct gatagaagag ttgcgccagc gtggtgtgaa
      301 tttcagaagc ctgactgaca gtattgatac cagtacccca atgggccgtt tcttttttca
      361 tgtcatgggt gccctggctg aaatggaacg cgaactgata gttgaacgta ccagggcagg
      421 gctggctgca gctcgtgcca aaggcagagt aggtggacgc cgtcctaagt tgaccaccga
      481 acagtgggca cagattgggc gtttactcga ggccggagaa tcaagacagc gtattgcact
      541 gatttttgat gtaggcgttt ctaccattta tagaaaattt ccggcaaata agatcaatga
      601 atccccctga atcagcatta tgttgattat ccctgcaagc agacaaatac cgttattttg
      661 tgtgaataac gacacaactg cgtttagctg tttgtcaggc acaatcactt caacataggg
      721 cgaagcctaa tccaatcagg aggttcgcca ctatggctca ggattaccac cacggggtgc
      781 gcgttgttga agtcaacgaa ggcacccgat ctattaccac ggtgagcacc gccatcgtgg
      841 gtatggtctg cacgggcgat gatgccgatg caaaaatgtt tcctcttaat aaacccgtgc
      901 tgatcactga tgtgctgact gccagcggta aagcgggtga gtccggtact ctggcccgtt
      961 cgctggatgc catcgctgac caggcaaaac ccgtgaccat tgttgtgcgt gtgccgcagg
     1021 gtgaaacgga agacgaaacc acgaccaata tcatcggcgc agtgactgct gaaggtaaaa
     1081 aaacaggtat gaaagccctg ttatctgccc agtcacagct cggcgttaaa ccgcgcattc
     1141 tcggcgtgcc aggccacgac accaaggcgg tggctactga gttgctgagc gtggcgcaaa
     1201 gcctgcgtgg atttgcttac ctgtcagcgt atggctgcaa gacagtgcag gaggcgatca
     1261 cttaccgcga aaacttcagc cagcgcgaag ggatgctgat ctggcctgac tttactggct
     1321 gggacacggt gctgaatgcc gaagcaacgg catatgccac cgctcgtgcg cttggtctgc
     1381 gcgccaaaat tgacgagcag accggatggc acaaaagcct gtccaacgtg ggcgtgaacg
     1441 gtgtcaccgg aatttctgca gatgtgttct gggatctgca ggacccggca accgatgcag
     1501 gtctgctgaa ccagaaagac gtcaccacgc ttgtgcgtaa agacggtttc cgcttctggg
     1561 gttcccgctg cctgagtgat gacccgctct ttgccttcga aaactacacc cgcacggcgc
     1621 aggtgctgat ggacacgatg gcagaagcac acatgtgggc ggtggataaa ccgcttaacc
     1681 cgtcgctggc ccgcgacatt atcgaaggta tccgcgccaa aatgcgcagc ctggtcagtc
     1741 agggctatct cattggtggt gattgctggc tggatgagtc ggtgaacgac aaagacacgc
     1801 tgaaagccgg aaaactcacc atcgactacg actacacgcc agtgccgcca cttgaaaacc
     1861 tgatgctgcg tcagcgcatc accgatcagt acctggtgaa tttcgccagc caggtcagcg
     1921 cgtaagggga caacatggct ttaccacgca aattaaaaca cctgaacctg tttaacgacg
     1981 ggaacaactg gcaggggatc gttgagtcgc tgacgctgcc gaaatttacc cgcaaatatg
     2041 agaagtatcg cggcggcgga atgccgggtg cggtggatgt ggatctgggg cttgatgaca
     2101 gtgcgctgga cacagaattt tccattggtg gtactgaatt gctgctgttt aaacagatgg
     2161 gtaaagccac ggtggatggc atccagctgc gctttaccgg ctctattcag cgtgacgata
     2221 ccggggaagt gcaggccgtg gagcttgtcg tgcgtggacg tcacaaagaa gtggattccg
     2281 gcgagtggaa gacgggcgaa agcaacacca ccaaagtgac cagtaccaac agctacgcga
     2341 agctgaccat caatggtgag gtgctctatg aagtggacct tatcaacatg gtggaaattg
     2401 tggacggtgt ggacctgatg gaagcgcacc gcaacgccct cggcctctga tatatctgaa
     2461 cggcgcggga taccgcgcca gaacccaatt gacaggacag caaaatgagc gataagcaga
     2521 ctgaaaagac cattcaactg gataccccca tcaagcgcgg taaaacagaa atcaccgaaa
     2581 ttgtgctgcg taaaccgcag tccggtgcgc tgcgcggtac acgcctgcag gccattatgg
     2641 atatggatgt aaacgcgatg atgaccgtga tcccccgcat ctccagtccg gcactgactg
     2701 cacaggaaat tgcagagatg gacccggcag atctcactgc catgtcggtt gaggttgtca
     2761 cttttttgtt gaagaagtcg gtgcttgccg gtttaccgac agcctgacgg ttgacgatct
     2821 ggtggcagat atcgccacca tttttcactg gccgccatcc gttactgacg ttatgccgct
     2881 gaccgaagtg ctggaatggc ggtataaagc gattcagaga agcggggcca acgatgagtg
     2941 ataacaacct gcgtctgcag gtcattctta atgcggttga caagcttacc cgcccatttc
     3001 gatctgcgca ggccagttca agagaactgg ctgctgctgt caaaaaatcc cgcgatgcaa
     3061 taaagcagct tgatcaggcc gggagcagtc tggacagctt ccgaaagctg caggcagaaa
     3121 atcagaaatt aggcgacagg ctgaactatg cccgccagcg tgcaaatttg ctcagtcagg
     3181 aactgggagc gatggggccg ccttcgcaac gtcaggttgt tgctctgggc cgtcaacggc
     3241 tggctgttca gcgcctggaa gaacgccaga aaaagctgca gcagcagacg gcgcttgtgc
     3301 gtgctgaact gtaccgggcg ggaatttctg cgaaagacga tgcgggagca actgcccgtt
     3361 tagcccgtga aacatcacgt tataaccagg aactttcgaa acaggaggcg cggctgaagc
     3421 gactggggga agctcagcgc aggatgaatg cagcgcgtgc cagttatgcc cgttcgctgg
     3481 aggtgcgtga tcgtattgca ggtgccggag ccaccaccac ggctgcaggg ctggcaatgg
     3541 gcgcaccagt gatggcggca gtaaaaagct ataccagcat ggaagatgcc atgaaaggtg
     3601 tggcaaagca ggtcaatggt ctgcgtgacg ataatggcaa ccgcactgca cgtttttatg
     3661 aaatgcagga tgccatcaag gctgccagcg aacagttgcc gatggaaaac ggtgcggtgg
     3721 atttcgctgc actggttgaa ggtggtgcgc gcatgaacgt cgcaaaccct gacgacagtt
     3781 gggaagatca gaaacgtgac ctgctggcct tcgccagtac ggcagcaaag gcggcaacag
     3841 cctttgagct gccagcggat gaactgtcag aaagtctggg gaaaatcgcc cagctctaca
     3901 aaatccccac ccgcaatatt gaacagctcg gcgatgcgct gaactatctg gatgataacg
     3961 ccatgtcgaa aggggcagac atcattgatg tgatgcaacg tctgggcggt gtggctgacc
     4021 gtctggatta tcgtaaagcg gcggcgctgg gttccacctt cctgacactg ggcgctgcgc
     4081 cagaggttgc agccagtgca gcaaacgcga tggtgcgtga attgtccatt gccaccatgc
     4141 aaagcaagag tttctttgaa gggatgaatc tgctgaaact caatcctgaa gtgattgaaa
     4201 agcagatgac gaaggatgcg atgggaacca tccagcgcgt gctggagaag gtaaacgcgc
     4261 tgccgcagga caagcgcctg tctgccatga ctatgttgtt tggtaaagag tttggcgatg
     4321 acgcggcgaa actggcaaac aacctgccgg aactgcagcg tcagttaaaa ctgacagcgg
     4381 gcaatgatgc gctcggctcc atgcagaaag aatccgacat taacaaggat tcactttctg
     4441 cgcagtggtt gcaggtcaaa accggagcgc agaacacctt cagcagtctg ggcgaaacgc
     4501 tgcgccagcc gctgatggat attctgtaca cggtgaaaag cgtcacgggg gcgttgcgtc
     4561 gctgggtgga agctaacccg gaactgacag gcacactgat gaaagcatcg gctgttgtgg
     4621 ctgcggttac cgtcggccta ggcaccttag cggtggcgat ggctgcagtg ctggggccgc
     4681 tggcagtgat ccgtctggga ttctctgtgc tgggtatcaa aacgttacct tccgttacgg
     4741 cagcagtaac tcgaaccagc aacgcgttgt cctggttggc tggcgctcca ctggcactgc
     4801 tgcgacgcgg gcttgcttca tcgggcaacg ccgcaggttt acttactgcg ccgttgtcgt
     4861 ctttgcgccg cacggcatca ctgacgggaa atgtcctgaa aactgtagca ggtgcgccag
     4921 ttgcactttt gcggtctgga ttatccggtt tacgtgctgt tgctgtgatg tttatgaatc
     4981 ctctggcggt actgcgcggt ggactggccg ccgcaggcac ggtgctgcga gtactggcat
     5041 ctggtccact ggcgatgctg cgcgttgccc tgtatgccat atctggtctg ttaggtgctc
     5101 tgctcagtcc gataggtctt gtggttactg cactggcggg cgtggcgctg gttgtctgga
     5161 aatactggca acccatcacc gcatttctcg gtggcgtggt ggaaggattc aaagcggcgg
     5221 caggtcccat cagtgcagcg ttcgaaccgc ttaagcccgt gttccagtgg attggcgaca
     5281 aagtgcaggc gctgtggggc tggtttactg atctgctgac gcccgttaag tcgacctctg
     5341 ccgaactgca gagcgcagcg gcaatggggc ggcgattcgg ggaggcactg gcggaagggc
     5401 tgaatatggt catgcatccg ctggactccc tgaaatccgg cgtttcctgg ttgctggaga
     5461 agctcggcat tgtcagtaaa gaggccgcaa aggcaaaact gccggaaagc gtgacgcgtc
     5521 agcaacctgc gacggtgaat gcagacggta aagtgatgat gccatcgggt ggttttccat
     5581 catggggata tggctttgcg gggatgtatg acagcggcgg gtatatcccg cgcgggcagt
     5641 ttggcatcgt cggtgaaaac ggaccggaaa ttgttaacgg cccggcaaat gtgaccagcc
     5701 ggagaaatac agctgcactg gctgccgttg ttgccggaat gatgggcgtt gctgccgcgc
     5761 cagcagagct tccaccgttg caccccttgg cacttcccgc gaaaggtgga gaagcaattg
     5821 tgagtcgcgc agccactgtg ccgctcgttc aacggattga ggcaccgacg cagatcatca
     5881 ttcagacgca gccaggacaa agtgcgcagg atattgcgcg ggaggtggca cgccagcttg
     5941 atgaacgtga acgcaggctg aaggcaaaag ccaggagtaa ctacagcgat caggggggat
     6001 acgacgcatg atgatggtgc tgggattgta cgtgtttatg ctgcgcaccg ttccgtatca
     6061 ggaactgcag tatcaacgca gctggcgaca tgcggcaaac agtcgggtaa accgacgtcc
     6121 gtccacgcag tttctgggac cggaaaacga catgctgacg ctttccggtg ttcttatgcc
     6181 ggaaataaca ggcggcaggc tgtcgttgct ggcactggag cagatggcag aacaggggaa
     6241 agcatggccc ctgattgaag gcagcggcac gatttacggc atgtatgtga ttgagggact
     6301 gaatcagact aaaacggagt ttttccgcga cggtatgccg cgccggattg agttcaccct
     6361 gtcgctcaaa cgagtggatg aatccctgtc cgatatgttc ggtgatctca gtgcgcaact
     6421 gaataatctg caggacacgg caacgtctgc cttaagcgat atcagtaaaa cggtgggagg
     6481 gctgctgtcg tgaatttcag ctctgaactg cttaacaaag gcaacaaaac tcccgcattc
     6541 agcatcagta ttgagggcag ggatatcacc actgtgctgg ataaccgcct gatgagtttg
     6601 acgctgacgg ataaccgggg ctttgaagcg gaccagcttg atctggagct ggatgacgct
     6661 gacggaaaaa tcgtgctgcc gcgccgtggt gcggttatta cgctggcgct gggctggaag
     6721 gggcagccgc ttttcccgaa aggggcattc acagtggacg agattgaaca cactggcgca
     6781 ccggaccgcc tgactatccg ggcgcgaagt gctgatttta gggaaacgct gaatacccgc
     6841 cgtgaaaaat cgtggcacaa gaccactgtc ggggaagtgg tgaaggaaat agccgcgcgt
     6901 cataagctga agatggcact gggtaaagac ctgtcggata agcccgtgga gcatatagac
     6961 cagactaatg agagtgacgg cagttttctg atgcggctgg cgcgccagta cggtgccatc
     7021 gcgtcggtga aaaatggcaa tctgttattc atccggcagg gacagggcaa aagcgccagc
     7081 ggtaaaccac tgccggtgat ctcaatcaca cgtaaggacg gcgacagtca ccgctttacc
     7141 ctggcagatc gcggagccta cacgggcgta attgccagct ggttgcatac ccgcgaaccc
     7201 gcgaagaaag aaagcaccac ggtgaagcgt aagcgcagga ctaagaagca gaagaaagag
     7261 ccggaagcga agcagggcga ttacctggtg ggtacagatg aaaacgtgct ggtacttaat
     7321 cgcacctatg ccaaccggag caacgccgaa cgggcggcga aaatgcagtg ggaacgcctg
     7381 caacgcggcg ttgcgtcatt ctcgctacaa ctggcagaag gtcgggcaga tctctacacg
     7441 gaaatgccag tgaaagtcag tggctttaaa cagccgatag atgatgcgga atggaccatt
     7501 acgactctga cgcatactgt cagcccggat aacggtttta cgaccagttt ggagcttgaa
     7561 gtgaagattg atgatttcga aatggaatga ttcttcgcaa tggagaactt ttaagttttc
     7621 aaaatggaat aatgcggtat cattattgtg aatttagcaa aaatggggag aactcgaaaa
     7681 atgatgattt gcccactgtg tggaagtgcc gcccatactc gcagcagttt tcaggtatct
     7741 tcattgacca aagagcgtta caaccagtgc cagaacatta actgcagcca tacttttgtt
     7801 acccatgaaa cttttgttcg ttcgattgca acgccaaaag agtcaaatcc ggttcagccg
     7861 catccaatga aatcaggaca ggtggcgctc tctctttgac gctgccgcca ttttgtcgcc
     7921 atcgttaaaa aacagtgttt ctaacatcat gattttaaac agcttaaatt tcaggcaaca
     7981 aaaaacccat caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca
     8041 tcaaagaaaa gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt
     8101 gcaaaaaaat ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga
     8161 cgatgattgt ccctgccagc gtcagcagga cgttggcgat tgcataggtg cccgcatagc
     8221 ccagcgccgg gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg
     8281 tacgtgcgcc catcattgcg ccgaacaaca gcgcgcggtt cattcgcaat acataagcac
     8341 cgaacaagaa acagataacc acgggcacca gactgacaat caatccggca atcaacatct
     8401 gaccgccaat cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg
     8461 ccataaacac catcaagccg aactctttca ccatgcttaa tgcaccctgc ggaatgtaac
     8521 cgaaggtcgg gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg
     8581 cagcgttccc catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca
     8641 gcccaataac aaagaaggcg cagaatgcca gcaggtcagt gacctggctg tgaatcgaga
     8701 taaagccgat gcgatcggcg atggttttta cgcggcgggc atcgccgctg acttgtaaaa
     8761 cgtcaccttt gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga
     8821 cgcggttaag gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag
     8881 cgttatggtt tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat
     8941 cgaaaacttc tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc
     9001 ctaccaacgc tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa
     9061 tcccgttacg tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac
     9121 gcagattttt gccgtcggtc caggccacca gttccgggcc gacgcgatag gcgcggatca
     9181 ccggtaaata aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct
     9241 gggcgctggt ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca
     9301 aactcaccag accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca
     9361 gtgagagctg cctgctttcc atgccggaat gacgcagtgt atcgccagca ccgaccagaa
     9421 ccggtgtcga cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa
     9481 acagcttacc taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca
     9541 ttaggtaatt tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc
     9601 cgacgcagaa aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat
     9661 gttgttggcc taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg
     9721 aaccaagtcg taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa
     9781 tgtaattccc atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta
     9841 ctagtaagct gttgaaagaa atggtaattt acgataatgt tttttaccag aattcagggc
     9901 gcagattcat tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat
     9961 cacattaggt atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag
    10021 cggctttatc ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag
    10081 gaggaacgag tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc
    10141 attgtggtgt gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat
    10201 cctgaaatcg gcttgctatt tttcattctt cctggagcag tcgccagctt cttttcacag
    10261 cgtagagaag tcctgaaacc tctgtttggc gcaatgctgg cggcaccctg ttcgatgctc
    10321 attatgcggc tgtttttttc accgacgcgc tcattctggc aagagctggc atggttatta
    10381 agtgcggtgt tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt
    10441 aaaccacagc acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag
    10501 gcgtccagat tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga
    10561 tcgacaaaaa tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct
    10621 ttagtgatcc cttccgcacg aatatctaca tagtgatact gaaaatcatc gtgttcattg
    10681 ctcaatttct cagccagatc ttttgcacgc acacagtaag ggcaacccgg acgaccaaaa
    10741 ataacggttt gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat
    10801 gtattcgctg aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa
    10861 cacaatacag tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg
    10921 cgatcggtaa attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg
    10981 cggtggcgct gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag
    11041 aagtgcagat tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg
    11101 ttattcttca ggtggcaaaa cgtctttccc cacagctgat gaaccgtcca ccgcaatatt
    11161 cacgttcaga aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc
    11221 tccattcgcc atttcactga tgaacccatt tccgaagcgc agcgtgaggc gattattaac
    11281 agcgcccgtg cgacgtccag ttccagtttt ttgcagtgca gtagcattat tcgcattacc
    11341 gacaaagcgt tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa
    11401 gcggcggagt tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat
    11461 gctcagctcg gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg
    11521 cagaatgcat taatcgcagc ggaatcgctg ggattgggcg ggtatatatc ggcggcctgc
    11581 gcaataatat tgaagcggtg acgaaactgc tgaaattacc gcagcatgtt ctgccgctgt
    11641 ttgggctgtg ccttggctgg cctgcggata atccggatct taagccgcgt ttaccgtcct
    11701 ccattttggt gcatgaaaac agctatcaac cgctggataa agacgcactg gcgcagtatg
    11761 acgaacaact ggctgaatat tacctcaccc gtggcagcaa taatcgccgg gatacctgga
    11821 gcgatcatat ccgccgaaca atcattaaag aaagccgacc atttattctg gattatttgc
    11881 acaaacaggg ttgggcgacg cgctaaaacc gccacgtcga tgtatgatac gcgggctttt
    11941 gaccaggtct gacagagagg tgcagggtga aaattgccat attgtcccgg gatggaacgc
    12001 tctattcgtg taagcggctg cgtgaagccg ctatacagcg cggtcacctg gttgaaattc
    12061 ttgatccgct ttcttgctac atgaacataa atcctgcggc gtcttctatt cactacaaag
    12121 gccgcaagtt accccatttt gacgcagtga tcccgcgtat tggcaccgcc attacctttt
    12181 atgggacggc ggcactgcgc cagttcgaga tgctggggag ctatccgctc aatgagtcgg
    12241 tcgccattgc ccgggcgcgt gacaaattgc gttccatgca actgctggcg cgtcagggca
    12301 tcgacctgcc tgtcacgggc attgcgcatt cgccggatga taccagcgat ttaatcgaca
    12361 tggtcggtgg tgcgccgctg gtggtcaagt tggttgaagg cacgcaggga attggcgtcg
    12421 tgctggcgga gacgcgtcag gcggcggaaa gcgtgattga cgctttccgc ggtctgaacg
    12481 cgcatattct ggtgcaggaa tatatcaaag aggcgcaagg gtgcgatatc cgctgtctgg
    12541 ttgttggcga tgaagtggtc gctgcgattg aacggcgggc gaaagagggc gattttcgtt
    12601 ccaatttgca tcgtggcggc gcggcaagcg tcgccagtat cacaccacag gagcgtgaaa
    12661 tcgcgataaa agccgcgcga acgatggcgc tggacgttgc tggtgtggat attctgcgtg
    12721 ctaatcgcgg gccgttggtg atggaagtga atgcgtcgcc ggggctggaa ggaatagaaa
    12781 aaaccactgg tatcgacatc gcgggtaaaa tgatccgctg gatcgaacgc cacgctacga
    12841 cagaatattg cctgaaaacg ggtggttagt cgcaatcaca ttactgatca tggttttgcc
    12901 tgcgcttttt gcgtaagctg tgccggtctt tttatcgaaa gaggttgtac aaaattatga
    12961 catcgctggt cgttcctggt ctggatacgc tgcgtcaatg gctcgatgac ctggggatga
    13021 gtttttttga atgtgataac tgtcaggctc tgcatctgcc ccatatgcag aatttcgacg
    13081 gtgtctttga tgccaaaatc gatctgatcg ataacacgat cctgttttct gccatggcgg
    13141 aagtccgacc ttcagccgta ttgccgctgg cggcggattt atctgccatc aatgccagtt
    13201 cgctgaccgt gaaagcattt cttgatatgc aggatgataa tctgccaaag ctggtggttt
    13261 gccagtcttt atccgttatg cagggcgtaa cctatgagca gtttgcatgg ttcgtgcgtc
    13321 agagcgaaga gcagatttcg atggtcattc ttgaagctaa tgcccatcaa ctgctgttac
    13381 cgactgatga tgaagggcaa aacaacgtta ccgaaaacta tttcctccac tgataactcc
    13441 tttcgagcac gcagtcgctg gtgcagtggc tgcgcgctgc aaaattatct gctgttttta
    13501 accttttctt aaagattatt tcacttctct tgtgtcgatt tggctttatc acatagagca
    13561 aatatgcata aaaatttgtt aaataccgtt ttttaatccg agctatagtc tcaaaccctg
    13621 gctaaagtta ttcttgcgat gcttttatat agcgagcagt gctggccggg agagagttct
    13681 cttttcttac accgcgccga taaaaaatat gcacgtttat tgcatatctt ttagtgtgac
    13741 aacttttgtt cgtttgttaa cgaactttca gaaggaaaga gatatgaccg ccttaaataa
    13801 aaaatggctt tcgggtctgg ttgcgggtgc tctgatggcc gtctctgtcg gcacgctcgc
    13861 ggctgaacaa aaaacactcc acatttataa ctggtctgat tatatcgccc cggacacggt
    13921 ggccaatttt gaaaaagaaa ccggtattaa agtcgtctac gatgttttcg actctaacga
    13981 agtactggaa ggcaaattaa tggcgggtag caccggcttt gacttggtgg ttccatctgc
    14041 cagctttctt gaacgccagt tgactgcggg agttttccag ccgctggaca aaagcaaatt
    14101 gccggagtgg aagaatctcg acccggagct gctgaagctg gtcgccaaac acgatcccga
    14161 caataaattt gctatgccct atatgtgggc gacgaccggg attggctata acgttgataa
    14221 agttaaagct gtgctgggcg aaaacgcgcc cgtcgatagc tgggacttga tcctcaaacc
    14281 tgaaaatctg gaaaaactga aaagctgcgg tgtctctttc ctggatgcgc cagaagaagt
    14341 ttttgctacc gtgttgaatt atctcggcaa agatcccaac agcactaaag cggatgatta
    14401 caccggaccg gcaaccgatc tgctgttaaa gctgcgcccg aacattcgtt atttccattc
    14461 atctcaatac attaacgacc tggcaaacgg cgatatttgc gtcgctatcg gctgggcagg
    14521 tgatgtctgg caggcgtcaa accgcgcgaa ggaagcgaag aatggagtga atgtctcgtt
    14581 ctcgattcca aaagaagggg cgatggcgtt ctttgatgta ttcgccatgc ctgcggatgc
    14641 caaaaacaaa gacgaagcct atcagttcct gaattacctg ctgcgcccgg atgtagtagc
    14701 gcatatttcc gaccatgtgt tctatgccaa cgccaataaa gcagccacgc cgctggtgag
    14761 tgcggaagtc cgtgagaacc cgggtattta tccgcctgcg gatgttcgtg cgaagctgtt
    14821 cactctgaaa gtgcaggacc cgaaaatcga ccgtgtgcgc acccgcgcgt ggaccaaagt
    14881 gaagagcgga aaataatccg cagccttaga tgccgggcgg gcgcaccaca cccgccggca
    14941 attcgcacca tcatggtgcg cttgcacaca ttcaatgccg gagagcagcc gtgaatgacg
    15001 ctatccctcg cccgcaggcg aaaacccgta aggcgctgac gccgctatta gaaatccgca
    15061 acctcaccaa atcctacgat ggtcaacatg cggtggatga tgtcagcctg actatctaca
    15121 aaggtgaaat cttcgcgctg ctgggcgcat ccggctgtgg caagtccacg ctgctgcgta
    15181 tgctggcagg tttcgaacaa ccttctgccg gacagataat gcttgatggc gtcgatttgt
    15241 cacaggttcc gccttacctg cgccccatca atatgatgtt tcagtcttac gcgctgtttc
    15301 cgcatatgac cgtggaacag aacatcgctt ttggcctgaa acaggacaaa ctaccgaaag
    15361 cggaaattgc cagtcgggtc aatgagatgc tcggcctggt gcacatgcag gagttcgcca
    15421 aacgcaaacc gcatcagctt tccggtggtc agcgacagcg tgtggccctg gcccgaagcc
    15481 ttgccaaacg cccgaaacta ttactgctcg atgagccgat gggcgcgctg gataaaaagc
    15541 tgcgtgaccg gatgcagctt gaagtggtgg atattctgga gcgcgtcggt gtgacttgtg
    15601 tgatggtcac ccacgatcag gaagaggcga tgaccatggc ggggcgtatc gccattatga
    15661 atcgcgggaa atttgtccag attggcgagc cggaagagat ctacgagcat ccaactaccc
    15721 gctacagcgc tgaatttatc ggttcggtta atgtctttga aggtgtactc aaagagcgtc
    15781 aggaagatgg cctggtgctt gattcgccgg ggctggtgca tccactgaaa gtcgatgcgg
    15841 atgcttcagt ggtcgataac gtgccggtac acgtagcgct gcgcccggaa aaaatcatgc
    15901 tttgcgaaga gccgcccgcc aatggttgta acttcgcggt gggggaggtg atacacattg
    15961 cctatctcgg cgatctttcg gtgtatcacg ttcgactgaa aagtgggcag atgatcagcg
    16021 cccagctaca aaacgcccat cgtcatcgta aagggttacc gacctggggc gacgaagtgc
    16081 gtttgtgctg ggaagtggac agctgtgtgg tgctgacggt ttaaggagca aagatgagta
    16141 cacttgaacc tgctgcccag tcgaaaccgc cgggcggatt taagctgtgg ttgtcgcagc
    16201 tgcaaatgaa gcatgggcgc aaactggtca ttgcgttgcc atatatctgg ttgatcttgc
    16261 tgtttctgct gccatttctg attgtcttta aaataagcct gggagagatg gcgcgcgcta
    16321 ttccacctta taccgaactg atggagtggg ctgacgggca actttccatc actcttaatc
    16381 tcggtaattt tctgcaactg accgacgatc cgctctattt tgatgcttat ctccagtcgt
    16441 tacaggtggc ggcgatttcg actatttgct gtttactgat cggctatccg ctggcgtggg
    16501 cggtggcgca cagtaagcct tcgacccgta atattttatt actactggtg atcctgccgt
    16561 cgtggacctc gtttctgatc cgcgtttatg cctggatggg aatattaaaa aacaacggtg
    16621 tgctgaataa ttttctgctg tggctggggg ttatcgatca accgctgacc attctgcata
    16681 ccaatctggc cgtttatatc ggcattgttt acgcttacgt gccgtttatg gtactgccga
    16741 tttataccgc gttgattcgt attgattatt cgctggtgga agcagcgctg gatctcggtg
    16801 cacgaccgct gaaaacgttc tttactgtga tcgtgccgct gactaaaggt gggattattg
    16861 ccggatcgat gctggtgttt atcccggctg tgggcgagtt tgtgatcccg gaactgctcg
    16921 gtggcccgga cagcatcatg atcgggcgcg tgctgtggca ggagttcttt aacaaccgcg
    16981 actggccggt ggcctcggcg gtagcgatca tcatgttgct gctgctaatt gtgccgataa
    17041 tgtggtttca caaacaccag caaaaaagcg tgggagaaca cggatgaata atttaccggt
    17101 agttcgttcg ccctggcgga ttgtgatttt gctgttgggc ttcacctttc tctacgcgcc
    17161 aatgctgatg ctggtgatct attcattcaa cagctcgaaa ctggtgacgg tgtgggccgg
    17221 ctggtcaacg cgctggtatg gtgagttatt gcgcgatgac gcgatgatga gtgcggttgg
    17281 tttaagcctg acaattgctg cctgtgcggc aacggcggcg gcgatcctcg ggactattgc
    17341 ggcggtggtg ctggtgcgct ttggcaggtt tcgcggatca aatggctttg cctttatgat
    17401 caccgcgccg ctggtgatgc cagatgtcat cacgggcttg tcgctgttgt tgttattcgt
    17461 cgcgcttgct catgccattg gctggcctgc ggaccgcggg atgcttacca tctggctggc
    17521 gcatgtcacg ttctgtacgg cttatgtggc agtcgttatt tcgtcgcgtc tgcgggaact
    17581 ggatagctcg atagaagaag cagcgatgga tctcggtgcg acgccgctga aagtattttt
    17641 cgtcattacg ctaccgatga tcatgcccgc gatcatttct ggctggttac tggcttttac
    17701 tttgtcgctt gatgatctgg tgatcgccag ctttgtttct gggccgggag ccaccacgtt
    17761 accgatgctg gtcttttcca gcgtgcggat gggggtgaat ccggaaatca acgcccttgc
    17821 aacgttaatt ctcggtgcgg tcggaattgt cggatttatc gcctggtatc tgatggctcg
    17881 cgcagaaaaa cagcggatac gcgatatcca gcgtgcaaga cgtggctgaa gacactaaaa
    17941 tttgccaacc tggctacata atgccgcgca tgccgcggca ttgttttcat ggaagacgaa
    18001 acgttgggat tttttaagaa aacatcttca tctcatgctc gcctgaatgt gcctgcgctg
    18061 gtgcaggtag cggcgctcgc cattattatg atccgtggcc tcgacgtgct gatgattttc
    18121 aatacgctgg gcgtgcgcgg tattggcgag ttcattcatc gcagcgtaca aacctggagc
    18181 ttaacgctgg tctttttaag cagtctggtg ctggttttta tcgagatctg gtgtgcgttt
    18241 tcactggtga aagggcgtcg ctgggcgcgc tggctatatc tgctgacaca aatcaccgcc
    18301 gcaagttact tgtgggcggc ttcgctgggg tatggttatc cggagctgtt cagcattccc
    18361 ggtgaatcaa aacgtgaaat cttccatagc ctgatgctgc agaagctgcc ggatatgctc
    18421 atcctgatgc tgctgttcgt tccctcgacc agtcggcggt tcttccagtt gcaataatgt
    18481 gtataatcgt cgcccctgat gatgtgaagg tcaatgtatg cagtgcgcac tttacgacgc
    18541 gggtcgctgt cgttcctgtc agtggataac gcagccgatt ccagagcaac tctccgctaa
    18601 aaccgccgat cttaaaaatc tgctcgccga ctttccggtt gaggaatggt gcgcgccggt
    18661 gtcaggcccg gaacaagggt ttcgtaataa agccaaaatg gtggtgagtg gtagcgttga
    18721 aaaaccactg ctcggtatgc tgcatcgaga cggcacaccg gaagaccttt gtgactgccc
    18781 gctttatcct gcctcatttg cgcccgtttt tgcggcgcta aaaccgttta tcgcccgagc
    18841 ggggttaacg ccctacaacg tggcgcgtaa gcgtggcgaa ctgaaataca ttttgctgac
    18901 tgaaagccag agcgatggcg gcatgatgct gcgttttgtg ttgcgttccg aaaccaaact
    18961 ggcgcaactg cgtaaggcgc taccgtggtt acaggaacaa ctaccgcagc taaaagttat
    19021 taccgtcaat attcagccgg tacatatggc gattatggaa ggggagacgg agatctacct
    19081 gaccgaacaa caggcactgg cggagcgttt taacgatgtg ccgctgtggg tccgtccgca
    19141 aagtttcttc cagactaatc cggcggtcgc cagccagctg tacgctaccg cgcgcgactg
    19201 ggtacgacag ctgccggtta aacatatgtg ggatctgttc tgtggcgtgg ggggctttgg
    19261 tttacactgc gcgacgcctg acatgcagtt aaccgggatc gaaattgcgc cagaggccat
    19321 tgcctgtgca aagcagtcag ccgctgaact gggcttaacg cgtttgcaat ttcaggcgct
    19381 ggactccact cagtttgcca ctgctcaggg ggaggtgccg gagctggtgc tggttaaccc
    19441 gccgcgccgc ggcattggta aaccgctgtg tgattatctc tcaacgatgg caccgcgttt
    19501 tatcatctac tccagctgta acgcccaaac catggcgaaa gatgtccgag aactgcctgg
    19561 ttaccgcatc gaacgggtac agctttttga tatgttcccg cacaccgcgc actatgaagt
    19621 gctgacgctg ctggtgaagc aataaaaaag ccgcaggtgc ggcttcagat tgctgacaaa
    19681 gtgcgcgttg tttatgccgg atgcggcgta aacgccttat ccggcctaca aaagcgtgca
    19741 aattcaatac attgcatggg ccatgtaggc ctgataagcg tagcgcatca ggcaatttta
    19801 cctttgtcat cagtctcaag ccgcggttgc ggctttctga atcttactgc gggaaccact
    19861 ggtcattgat tttctgatat gtaccgtcag ctttaattgc tgccagcgcg ttattcagtt
    19921 tttccagcag agctttgtta tccggacgta cagcgatgcc cagaccggtg ccgaagtatt
    19981 gcggatcggt cactttttca gtagcaacgc ccagttgcgg attggttttc agccattcgt
    20041 ttaccaccgc tgtgtcacca aataccccat caatacgacc atttttcaga tcgataaagg
    20101 cattctgata actgtcatag gagacagttt tcacttcggg gtgctgatcc tgaatatatt
    20161 tctggtgcgt ggtgccgttt tccatcccaa tacgtttgcc tttcagatcg gcaaacgttt
    20221 tgtaggtatc ttttttggca atcacgacgg ctgagttttc atagtagggc gtggtaaacg
    20281 atacctgctt gctacgctcg ggcgtgatat ccataccgga gattacggcg tcatattttc
    20341 tgaatttcag tgacgggatc aggctgtcga acgcgtgatt agtaaaagta cattctgcct
    20401 gcatttgttt gcacaaggct tttgccagat cgatatcaaa gccgacaatc tcattattag
    20461 cacctataga ttcaaagggg ggataggtgg ctgaaacgcc aaaattgatt ttctctgcgg
    20521 cagaagcacc gaaagtaaag gaagcaagta aagcggcaag aactaacttt ttcatgatgg
    20581 aactcccgtc tgtcaatctt atgatttttg gccgtgtctg cggcatggga taacaatgcc
    20641 atcaagtgaa tttatatgca ataaatatga ttaaataatt taaatgaaat aaaaaaagac
    20701 ggataactta gtgagttgtc cgtctttatt ataagaattt atgcactgtg taggccggat
    20761 aaggcgttca cgccgcatcc ggcatcagca tgcagctaca ttaattccgc cgttcgaatg
    20821 ccagtgcttt gcgctcgatc agacgcatca tcagcgtcag caggccgttg acgaccaggt
    20881 aaataatccc tgccgcaccg aacaccatta catcgtaggt gcgtccgtac aacaactggc
    20941 tgtatcccat cacttccatc agcgtaatgg tgtaagccag agaggtactt ttgaacacca
    21001 gcaccacttc gttggaataa gaagagagcg agcgtttaaa ggcatacggc agcaggatcg
    21061 ccagcgtatc ttttttgctc attcccaggg cgctacagga ctgccactga ccttctggga
    21121 tcgcacgaat tgcaccgtaa aacagctgcg tggtatacgc cgcactgttc agcgataacg
    21181 caatcagcgc acataaccac ggttctgaca acaaatgcca cagtgccgga tactcctgca
    21241 aagtcggaaa ctggcccggc ccgtaataaa tcaggaagat ctgcaccagc agcggcgtac
    21301 cggtaaacag cgtgatataa ccccgcacca gccacaccag caccggcgtt ttcagcgtca
    21361 ggatgatggt aaaaatcaat gccagaatca gtgccacaat cagcgaggca acggttagcg
    21421 tcaggctggt gtgtagccct ttcatcagtt cgggtaaata ctcaaacatt agctgggcct
    21481 ccgctcaaaa cgtgtcgcgc gcaggtcaat gcgtttgaga atgtactgac tgagcagggt
    21541 gatcaccagg taaatcgccg ccgccacaat gtaccaggta aatggttcct gggtacgagt
    21601 agcgatgctt tttgtttgca gcattaaatc attcacacta atcaaactga ccagcgcggt
    21661 atctttcagc agcaccagcc actggttacc gaggccaggc agcgcatgac gccacatctg
    21721 cggcatcacc agacggaaaa agatagccga tttcgacagc cccagcgcct gaccagactc
    21781 ccactgaccc accggcaccg ctttcaacgc gccccgaagc gtttgcgagg cataggcggc
    21841 atacagcagt gacagagcga tgacaccaca aaggaacggg ctaacgtcga agttctcaat
    21901 gtccatctgc actgggatct gcacgaaccc aagattgata gtgaagccat ccgaaagcgt
    21961 cagcagcagc tgcgaggagc caaaatagat aaacagcacc accagaattt ctggcaggcc
    22021 acgcagaatg gttaccagcg ctgaacctgc ccacgcgaca ggacgccatt ttgccgactc
    22081 ccataccgca aagaacatcg ccagcgccag cccgacaatc aatgcacaaa cggcaaggtc
    22141 gacggtcatc ccggcggcgc ttgctaaagg aaaaaattca ttcatcagga attacttctg
    22201 gaaccatttg ttgtagatgg tttcgtaagt gccatctttc ttcacttttt ccagcgcagt
    22261 gttgagtttc tgctgcagct cagtgttgcc ctgacgtacc gcgatgccga ggccagtgcc
    22321 gaagtaatct ttatcggtca ctttgtcgcc caccgccgcc agtttcgggt tatctttcag
    22381 ccactcagtg accactgcgg tgtcaccgaa gacgccgtcg atacgcccgt tttgcagatc
    22441 cagttttgcg ttctggtagc tgtcatacgg aacggtagtg atttccgggt gcttatccat
    22501 aatgaatttc tggtgtgtcg tcccgttctg tacgccgact tttttgcctt tcagctgatc
    22561 aacactggtg tatttgcctt gctgacccac aaacagggca gagttgtcat agtacggggt
    22621 ggtaaacagc acctgctttt cacgctccgg agtgatatcc atgcccgcca tcacggcttc
    22681 tacgcgacgg aatttcaggc ttgggatcag gctgtcaaac gcctggttag agaaagtgca
    22741 ggttgcatca atctctttac acagcgcttg tgccaggtcg acgtcaaaac caacgatctg
    22801 gttgtttgca tcaatcgatt caaacggagg ataggaggct tcggtagcaa aacgaatggt
    22861 ttcggcagct gtggcggaaa gactaaaacc tgcaattaac gcggcaatca gaactttttt
    22921 cattgttgtt atcccgaatc ttagtgagag agatagtttt taaatgcttc ggtttgcggc
    22981 tcggtaaagc agctcgcgtc gccttgttct acgatatgac cattttccat atacaccact
    23041 cggctggcgg ttttacgcgc cacttcaact tcgtgggtga cgatcacctg ggtaatattc
    23101 gtttctgcca gctcacgaat gatgctgacg atttgtgccg taatttccgg gtccagtgcg
    23161 gcggtcggtt catcgaacag cagtacctgc ggttccatca tcaacgcacg ggcaatagca
    23221 acacgctgct gctgaccacc agaaagatgc agcgggtaac gatcgctata aggtttgaga
    23281 cgcagacgtt ccagcagttt ttctgcacgg gccagcgcct gatctttact caaccccagt
    23341 acacggcagg gcgcttcaat caggttttgc tgcacggtca gatgcggcca caggttgtat
    23401 tgctgaaaca ccatgccaac gttacgacgc aaatcgcgaa tcgctttgtc agagggtgtt
    23461 ttggtgaaat cgaaatggtt gcctgcaatg ttgagcgtac cggagcgcgg catctcaagc
    23521 agattgagta cacgcagcag cgagctttta cccgcgccgc tggggccaag taacaccagc
    23581 gtttcgccct gtgggcaatc cagcgtgata tcgaacagcg cctgatgcgc gccgtagaag
    23641 caattaatgc cgtttaattg aatactcatt gacactcgta tactggcagt ctgatagcta
    23701 ttgaggtcga agatagtacc tttgacagaa taattatgca atatttctgc tttaaaagtt
    23761 aaaagcaaag cgcattattc aataaacata gcacaaaata acgggggcgg tggtcggcga
    23821 gcataaatgt cggcattcct cacgaaatgc cggacaattt acggggttta ttggttgatc
    23881 aaggcgttag cgattctcga tggactgacg gagcgtaccc gccgtggcat gaacgctacc
    23941 gcctaagtaa cgcacatcgt cgatgaccca acactggcct tcctgaatca ttaacacttc
    24001 atcctgccaa ccctggtcac cctgtttgag atccacgcgc aatggaatgt tacgggcatc
    24061 acgattaggg atagtcgatg cactggcaac gtgggcgcta tctggcaagg tggttcgact
    24121 ggagaatgga tcgttggtca gtagttcccg atggttatta tcccgggagg catcgctaag
    24181 cagtgtcgcc agtttgtcgc tcagataagg gcgcaaggcg gtgatgtcgt tgctgcggtg
    24241 caaaatgcgg tagtcataaa attgctgggc cacgttatcc gggcctcctt caacgcaagg
    24301 accactgcgt gtgccgttat ctttataagc tggagtgact gtggtgcagg cactgaggag
    24361 cagtgcgcag gggataagca ttgtcaattt gctgtagcgc ataatgattt ccttataagc
    24421 gatcgctctg aaagcgttct acgataataa tgatatcctt tcaataatag cgtatcagtc
    24481 tgataatgct tttgagatcg aaggcttagc aaacaaggag atcgatcatg caattttcta
    24541 caaccccaac tctggaaggc cagaccatcg ttgaatattg cggtgtggtg accggcgaag
    24601 cgattttagg tgccaatatt ttccgtgatt tctttgccgg tatccgcgat atcgttggcg
    24661 gacgttccgg tgcgtatgaa aaagaactgc gtaaagcacg ggagatcgcc tttgaggaat
    24721 taggctccca ggcgcgggcg ctgggggccg atgccgtcgt cggtattgat atcgactacg
    24781 aaacggtcgg gcaaaacggc agtatgctga tggttagcgt cagcggtacg gcggtgaaaa
    24841 cgcgtcgatg agaagagtct tctggctggt cgctgccgct ctgttattgg cagggtgtgc
    24901 aggcgaaaaa ggcattgtcg agaaagaggg atatcagctt gatacccgac gccaggcgca
    24961 ggcagcgtat ccgcgcatta aagtgctggt gatccactac accgcagatg attttgatag
    25021 ctcgctggcg acactgaccg ataagcaggt cagctcgcat tatctggtcc ctgcggtacc
    25081 accgcgatac aacggtaaac cgcgcatctg gcaactggtg ccggaacaag aactggcctg
    25141 gcatgcgggg attagcgcct ggcgcggggc aacgcgcctt aacgacacct ctattggcat
    25201 tgagctggaa aaccgtggct ggcaaaaatc ggccggagtg aaatattttg ccccgtttga
    25261 accggcacag attcaggcgc ttattccact ggcgaaagat attattgccc gttatcacat
    25321 caagccggaa aacgtagtgg cacatgcgga tatcgcaccg cagcgcaaag acgatccggg
    25381 gccattattt ccctggcagc aactggcgca gcaggggatt ggtgcctggc cggatgcgca
    25441 gcgggttaac ttttaccttg ccgggcgcgc gccgcacact cctgtagata ctacgtcatt
    25501 gctggagctt ttggcgcgct acggttatga cgttaaacct gatatgatac cgcgcgagca
    25561 gcggcgcgtg attatggcat tccagatgca tttccgcccg acgttatata acggcgaagc
    25621 ggatgcagaa actcaggcga ttgccgaagc attgctggag aaatacgggc aggattagcg
    25681 cggcagtttt ccgtggtcgc gtagccaggc ggcagttttc tcgatacctt catccagggt
    25741 gatgaccggc tgataaccta actcttcctg cgcacgcgta atatccagcg taaagtcaaa
    25801 attcaacttg gagacgccgt agtgggtcag cggcggctct tttgctgact tgcggcctaa
    25861 acgctccatg ctgcgggcga tcatatccag catcgggtag gggacggaac gaatacgaca
    25921 gtcaatattc aactcgtcga tcagcttctg cacgatgctg cgcagtgtgc gatgctcgcc
    25981 gttggtgatg ttgtacacac gcccggaagg tagcttatcg caggcttcct ggcttgccag
    26041 ccacattgcg tgcacggcat tttcatagta ggtcatatcg accagcgcac tgccgccatg
    26101 cggtaacaga atactgccgt agtggtgcat catatgcgcc agacggggaa taaagacttt
    26161 atcgtgcggt ccgaacagac tttgtgggcg cagaatagta aagcgcgttt gtggattcgc
    26221 ctgcgaaagc atattgatca cttcttcgct ggctgctttg ctgcgggcaa actcgttggc
    26281 gaagcggtga gggcgaaaat cttctttaat atcgcgatgg tggtgataat cgaagtacag
    26341 ggagggggaa gagatatgaa taaagttacg cacaccccag gcgacagccc attcacccag
    26401 gcggcgagtg gcgcgaacgt tagccagatc gaaagcctgt tgtgtccccc agggtgaggt
    26461 aaagctggag cagtgccaca gcgtatcaat gcccgcgagc atcactttag cttgtgatga
    26521 aaccagctcg gtcagatccg ccggaacaaa ctctgcgccc attttttcca gcaatttgcc
    26581 cattgcctcg ttgcgaccgg tcgctcgcac gctgatgcct ttctggcata aaaactctac
    26641 cgcgtttcga cctaagccgc tggtggcgcc ggtaaccagt accttcatat caatccactg
    26701 ttgttgagaa aataacgtgc gcattcttcc gtgatttccc ccatgatgca atgggaaaca
    26761 tgaaagaata acgcaggttt tgtcgattaa tctgtgcttt gttctgccag tctggcgatt
    26821 tgttttgcca ttccgcgaaa aataaacaga tgcgcgggga tcatcaataa ccagtaaaac
    26881 agccccggca taccgtgcgg atgccagaaa gcgcggacat cgatagtacg atagtcgcct
    26941 ttatcttcca ggctaaaaca cagtcgtccc agccccggcg ctttcatgcc aaataacaac
    27001 gtaagttgtt tttccggttc aacgacaatc actttccagc tatccaccgc atcgccagtc
    27061 tgtaaatatt cgcgctccgg gcggcctttc gccagcttat gaccgatcgc gcggtccatc
    27121 aacgcccgtg tctgccacaa aatattgcca aagaaataac gctctttacc gccgatttgg
    27181 ttcactacct gccataaagc agcaaggctg gcggacgttt taacggtaaa ccccgcctgt
    27241 ttggcaaaat aaccgtactc cggtcgccag cgagcaaagg cctgagcgtc gtagccccag
    27301 tcgctggagt tgaccagttt ttcctcctct ttcaacgtgc tacgtaccgc gtcatcgaaa
    27361 gcgatcagcc gttgtgggat gagtgcacgt agcgcggtat catccgccag cagatcgtgt
    27421 ttcagcccct gaatcaacgc cctggcggtg gtgggcggta cggaagtaat cacattgaga
    27481 aaccacaccg aaatccagcg ggtggggagg gggatgggga tcaaccagcg gcgcttaccg
    27541 ctcaccgcca taaaatgttc aaactgttgc tgataactga gcacctctgg tccggcggct
    27601 tcgaagatgc ggtgttcgct ggccggatga tctaacaacg ccaccagata gtgcagcaag
    27661 ttttccagcg cgatgggcgt ggtgcgtgaa cgtacccagc gtggcggcgt taacactggc
    27721 aggttgtaga ccatatcgcg catgacttcg aacgccgctg aacctgcgcc aacgataatt
    27781 ccggcccgaa gttcggtcac aggtacattc gcttcacgaa gaatgtccgc cgtagcctga
    27841 cgagcacgca gatgatccga ctgctcatgt ggcggggcct gcaacgaact gagaaagatt
    27901 aattgcttaa ctggtacttc acgtagcgca tcgcggacgt tgagagccac ctggcgctcc
    27961 tgagcgataa aatcgccgcc ttcgcccatg ctgtgcacca gaaaatagac ggtatcgata
    28021 tcctgcaaca gggccggaag gttatccggc cagctgagat cgactttatg gcaactgacg
    28081 tttgccagtt gcagctttgc aagcctgtcg acatgacgtg ccgccgccag gatctgatgc
    28141 ccttgctggc tgagtgtgcg caccagatgc tgaccaatgt agccactggc accgagaact
    28201 aaaatgcgtt gcggcacgtc tctctcctta acgcgccagg aatgcgcgcc agtgggcggc
    28261 gacttccgcc agttgttcgc gcgagacgtc aagatgcgtc accaggcgga caatcggcga
    28321 ggcgttaatc agcacgtttc tcgctttcat gtattcgcct aacgcggcag cattttcttc
    28381 cccgacgcga acaaacagca tattagtgtc ctgacgcatc acatccgcgc ctgcttcacg
    28441 cagttgctcc gccatccagg cggcgttgtc gtgatcttcc tgcaaccgtg cgacgttatt
    28501 tttcagggca tacatcccgg cggcagccag aatcccggac tgacgcatcc caccaccggc
    28561 cattttccgc cagcggatgg catgtttaat gtaatcacga ttaccgacga gtaatgaacc
    28621 gactggcgtc ccaagacctt tcgacaggca aatggtgaac gaatcacaat attgcgtgat
    28681 ctctttcagt tcgcagccgt aagccaccac ggcattaaag atgcgcgcac cgtcaacatg
    28741 cagcgccaga ttgcgctcgc gggtaaattc ccatgcttct ttcaggtatt cacgcggcag
    28801 cactttgccg ttgtgggtgt tttccagact gagtaatttg gtgcgggcga aatggatatc
    28861 gtcgggtttg attttcatcg ccactttatc cagcggtagc gtgccgtcgg cagccgcgtc
    28921 gatgggttgc ggctgaatac tgcccagcac cgctgcgcca ccggcttcaa acagatagtt
    28981 atgcgcggcc tgaccgacaa tatactcttc gccacgttcg cagtgactga gcagagcgac
    29041 caggttggcc tgagtgccgg taggcagaaa aatggcggct tctttaccgg aaagctctgc
    29101 tgcatagtcc tgcagagcat taacggtagg gtcgtctccg taaacgtcgt ccccaaccgg
    29161 ggcggccatc atcgcttcga gcatggcgcg gctcggtcgg gtaacggtat cactgcgtaa
    29221 atcaatcatg gcatgtcctt attatgacgg gaaatgccac cctttttacc ttagccagtt
    29281 cgttttcgcc agttcgatca cttcatcgcc gcgtccgctg atgattgcgc gcaacatata
    29341 caagctaaat cctttggcct gttcgagttt gatctgcggt ggaatggcta actcttcttt
    29401 ggcgaccacc acatccacca gcaccggacc gtcgatggag aaggcgcgtt gcagggcttc
    29461 atcaacttca gacgcttttt ctacacggat acccgtaatg ccgcacgctt cggcaatgcg
    29521 ggcaaagttt gtgtcgtgta gttcggtgcc gtcagtcaaa tagccaccag ctttcatctc
    29581 catcgccaca aagcccagca cgctgttgtt aaagacgaca attttcactg gcagtttcat
    29641 ctgcactact gagaggaaat cgcccatcaa catgctaaaa ccgccatcgc cgcacatggc
    29701 gaccacctga cgttctggct ctgtcgcctg cgcacccagc gcctgcggca tggcgttagc
    29761 catcgaaccg tggttaaacg aacctaacag gcgacgcttg ccgttcattt ttagataacg
    29821 tgccgcccac accgttggcg taccaacgtc acaggtgaaa atagcgtcat cggcggcaaa
    29881 atgactaatt tgctgcgcca gatattgcgg gtgaatggct ttctcgctcg gtttagctaa
    29941 atcgtccagc cctttgcggg cgtcgcggta atcttccagc gctttatcca gaaacttgcg
    30001 atcggctttt tcttccacca atggaagcaa tgcacgcaga gtcgacttga tatcgccgac
    30061 cagtgccata tccaccttgc tgtgagcgcc gatgctggct gggttgatat caatctgaat
    30121 gattttggca tcggtcgggt agaaggcgcg gtagggaaat tgcgtgccga gtagcactaa
    30181 cgtgtcggcg ttcatcatgg tatggaaacc tgacgagaag ccgattaacc cggtcattcc
    30241 aacatcatac ggattatcgt attcgacatg ttctttaccg cgcagggcat gaacaatagg
    30301 cgctttaatt ttcccggcaa actcaactaa ctctttatgc gcccccgcgc agccgctgcc
    30361 acacatcagg gcgatattgc tggaataacg cagcagttgc gccagtttgc gtaactcttc
    30421 ttcttccggc gtcacgactg gttgtggcgc atgataccag tgcgtggttg ccccttctgg
    30481 cgcaggtttt aacgccacgt cgcctggtaa cacgacaacc gaaacgccac ggttaagcac
    30541 cgctttgcgc atggcaatcg ccagtacttg tgggatctgc tccgggctgg aaaccagctc
    30601 gcaatagtga ctacattcgc ggaatagctc ttgtgggtgg gtttcctgga aatagccgct
    30661 gccaatttcg ctggagggaa tatgagcggc aatcgccagt accggaacgt gattgcggtg
    30721 gcaatcgaac aggccgttga ttaagtgcag gttgccgggg ccgcacgatc cggcgcagac
    30781 cgccagttct ccgctaagtt gtgcttcagc gccagcggca aaggccgcca cttcttcgtg
    30841 gcgggtggac atccactcga tggtgcccat gcgattaaga ctgtcactaa gaccgttcag
    30901 agagtcgcct gtgactcccc agatgcgttt cacccctgcc gattcgagtg ttttggcgat
    30961 ataagctgca accgtttgtt tcatggttct ccatctcctg aatgtgataa cggtaacaag
    31021 tttagttcat ctgacggagg gggaagggat gggagagaaa ggaggcacta acggttaaat
    31081 agcccgatga aaggaatatc atcgggcata aggcgattat gcgagaacca aatccccctg
    31141 cggatggcag gagcaggcca gtacgtaacc ttcagcgatt tcggcgtcgg tcagcgtcat
    31201 tgtactgctc accgtatatt caccggaaac cacttttgtc ttacagcagc cgcaaacacc
    31261 cgcacggcag gcagcgacaa ccggaacgtt attgctttcc agcgcctcca gtagcgtggt
    31321 gccaaccggg gcgtaaaatt ctcgtgccgg ttgcagtttg gtgaatttca gaccgctggt
    31381 cgccgcttct gctactgggg tgaagaattt ctctttaaag aaacgcgtca cgccgagcgc
    31441 tttcacttcc tgctctaccc aatccatata cggagccggg ccgcaggtca tcacggtacg
    31501 tgaagctaag tcaggtacac ctgccagcag ttcgcgagtg agacgaccag cgataaagcc
    31561 ttcggtaacg ttattttctg ccaccagcgt taccggatag ttacgccact catcggcgaa
    31621 aataacatcc tgcggcgtac gcacgttgta gatcacccgc acatcggcct gtggacggtt
    31681 cttcgcaagc cagcgacgca tcgacataat cggcgtgacg ccgcagcctg ccgccagcaa
    31741 caggaattta tcttctgctt tatcgtcgca ggtaaattcc cccatcgcgt ccgaaagcca
    31801 gagataatca ccgcgtttta catcgcgcgt cagccactgg gagccgacac cgtcatcaat
    31861 ccgccgcacg gtcagggtga tatattcact cacgcctggc gtggaggaaa tggtgtaagc
    31921 acgcagcgtt tccgctgagt ttcgcacgct gaccagtgca tattgcccgg cgcgatatgg
    31981 gtagtaatcg tggcaaatca gggaaatcgt ccacacatcc ggcgtttctt gcgtaatgtg
    32041 atgaacctgc atccgccacg ggcattgatt cgttggcatc gtcatcgaca aactccttac
    32101 gcgctcaaca gttgcttcat gtcttcttca acagtggtga tagaacgcag gccgaatttc
    32161 tcgttcagca ccgccagcag gtctggtgtc aggaaaccag gtgcagtcgg gccggtgacg
    32221 atatttttca cgcccagaga aagcagcgtc agcagaatga cgatcgcttt ctgttcaaac
    32281 caggagagca ccagcgacag cggcagatcg ttgacaccgc agcccagttt ctctgccaga
    32341 gtgacagcca gaataatcgc tgagtaagca tcgttacatt gacctgcatc taccagacgc
    32401 ggcagacctt cgatatcgcc aaactcaagt ttgttaaagc gatatttacc acaggcgagg
    32461 gtcaggatca ggcagtcatc cggcacgctg gtggcgaaat cggtgaagta gtggcgctcg
    32521 ccgcgtgcgc cgtcacagcc accaagcagg aagatatgac gcagtttttc acggctcacc
    32581 agatcaatca gcgtatcagc agcgccaagc agcgtctggc gaccaaaacc cacggtgata
    32641 aggtgcggaa tttcgctgta cgggaagcct gccatctgtt gcgcctgggt gataaccgca
    32701 gagaaatcat caccgtccag atgacgcacg ccaggccagc caacaatgct gcgggtccag
    32761 atacgatcgt cataagcgcc tacggttggg tcgatgatgc agttcgaggt catcacgatg
    32821 gggccaggga aacgagcgaa ctccacttgc tgattctgcc agccgctgcc gtagttaccg
    32881 accagatgct tgaatttacg cagctccggg tagccatgcg caggcagcat ttcgccgtgg
    32941 gtgtagacat taacgcccgt gccttcggtc tgttccagca ggttgtagag atctttgaga
    33001 tcgtgaccgg aaatcagaat gcatttaccc gccgtcgctt tgacgttgac ctgggttggc
    33061 gtcgggtgac cgtatttacc ggtttcgcct gcatccagaa tgctcatcac tttgaagttc
    33121 atctggccga tttccattga acactcaaga agcgcgttca tatcggcagg ccaggtcccc
    33181 agccacgcca tgattttatg gtactgggca taaatatcgt tgtcgtattg accgagaaca
    33241 tgcgcgtgtt ccatataggc cgccgcacct ttcaggccat acaggcacag cagacgcagg
    33301 ccgagaatgt tttcgccaat cgccgcttta tctttgttag gggtaaattc tgctgcctga
    33361 cgttgcagct cgccgagatc atcgctcacc agttgcaggt cagccatcgg gttatcgacg
    33421 cgcgcgttgg catctacagc gaggcattgt gctttcagcg cctcgcgcaa ggcaatcgct
    33481 tcacgagcgt agccgacaat acgcggagaa tcgaagttaa cgttggtcag ggttgagaaa
    33541 aaggcacgtg gcgcgaagct gtcaacatcg tggttgatga tgccgtattc acgcgctttt
    33601 accgcccagg cagaaagccc ttgcagcgcc gcgatgagta aatcctgaag gtcagaagtt
    33661 tccgccgttt taccacacat cccctgcgcg tatgagcagc cgtttcctgc cggagtacgg
    33721 atagtttgtt cacattgcac acaaaacatg atcacacctt ttaaagttat atttaatata
    33781 catgtttaag gttaagacgc ttaacgcggg gataaaaggg atttttcatg caactttaag
    33841 ggagattgat ttagcgcaat tttggcggca gggatctacc gccagagagg tattacgcag
    33901 agaaaaaggc gatgaggatc ggcactaaca ggctaagaat aaaaccgtga acaattgccg
    33961 ccgggaccat atccagcccg ccagtacgtt gaagaacggg cagggtgaaa tccattgatg
    34021 tggcaccgca taagcccagt gcagtagagc ggctgcggcg aatcagccca gggatcaaca
    34081 taatagcaat cagttcacgg gccagatcat taaaaaacgc cgcgctcccg attaccggac
    34141 caaaagattc ggtcaataaa ataccggaaa gagaatacca gccgaaaccg gaggccattg
    34201 ccagcgcggt attgatgggg agatcaagaa taaaggcgtt aattaaacca ccaattaatg
    34261 aactgacaac caccaccacg gcgacaatca ttccccggcg attaaggaca atctgcttta
    34321 aggtcatgcc attattgcgc aactgaatac caacgaggaa aagtagcaaa attaacgtgt
    34381 attcactggc ttcggtcgcg tgttgtaaga aagccagtcc actcagacca atggcaaaac
    34441 caatcactac tacgccgcac agttttagcg actccagcgc catcgcaata cgcgacggga
    34501 gtttttcttg ctgatggtgg ttgcgccacg gcaggcctcg ctcgagccac atcagggcgg
    34561 caatattaca cagtaaaata acggtaatac tgacggcaga ataatgcaga atcgccaaca
    34621 ggttactggc gaggttatcg agaaacgcca gactgatacc cataaaaaag agaataaggt
    34681 aaaccatcca gcttaatagc tgattaataa cttttaacgc agcttgttgg cgaagcggaa
    34741 tgaggtaacc cacaatcagg ggaaccagaa tgattaacag cccagaaaac atgaaaaccc
    34801 agtccttgca aagatgaagt cgaaatgcgc gatgacacac tactgaaagc ggaaggacga
    34861 gtaaagttgc aattaaaagg aaatgttatg cataaggagc agtagagtat tcgttttcat
    34921 ttaaagatat tcttgcgctt taattacaaa ctgcaccgat gttggtggcg tcaaaatcgc
    34981 cgaggcgttc cctgaaggcc ggggcagccc acatggatgt gggctgaggg cgcgttttac
    35041 agggatgtta cctcgcgccc gacccggtag ccgtaaggga taagtcgagg gcaccgcgca
    35101 gcggcgattt tgttcgccag agcccggggg tgcagggggc ggcggcgatt ggccgccccc
    35161 tgcgcgctcc ttgcgccagt ggcaatatgt tgcttagctc atgaaaggag cgcaacaaga
    35221 tgatgaatca acatataaca acatcttaaa aaaaggcctg acattacgcc aggccttctg
    35281 cgttaattaa tcacgctttt ccagcagggt ccggtaaatc agaccaccga taatgccgcc
    35341 gacaattggc accacccaga agaaccacag ttgttctaat gcccagccgc cctggaagat
    35401 agcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta gtcaccggaa tactaattaa
    35461 gtgaatcagg gttaaggcca gaccaatagc gatcggcgca aaacctgccg gcgcgaattt
    35521 gtcggttgcg ccgtggatca ccaacaggaa acctgcactc aataccagtt caactaccag
    35581 cgcggaaagc atggaataac cgcctggtga atgctcgcca taaccgttag aagcaaaacc
    35641 gctggctgcc gcgtcaaaac ccgttttacc actggcaatt aaatacagca gcgccgctgc
    35701 aacaataccg ccgacaacct gggcaattac gtagccaacg acttcttttg ccggaaaacg
    35761 tccgccagcc cataaaccaa tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc
    35821 aacagcaaag gccatcgtca gaacggtcag accgaacgcc aacgccacgc cggcaaaacc
    35881 aatgcctaat tccgggaagc ctgcggccag tacagcacta ccacagccac caaaaacaag
    35941 ccagaaagta ccaaaacatt cagctgctaa ttttctgaac atatccacct caattaaaaa
    36001 ttgaccctgt gaaaaatatg gtcgttttat agggtcgtcg taaaaaagtg acgacggaaa
    36061 taatgcgcgg ctattttaaa aacgaaggcg agtcattcac cagataaata aatccagtaa
    36121 atttgattta gggcaacagc gggttgcccc atatagtcat ttgtctgatt gacagtgtag
    36181 tgcacgcaaa agatttaatc ctttaggcgt aataaaaaat aatttatcat gctaattatt
    36241 tgattttgtt gtttttgcag acttatcagc aagagggagt ataacgcgat tattcgctca
    36301 tttttcagac atttgccatg cttaaatgtg atgtcatcac gtattagcaa ggcctttccc
    36361 gttatactgc cagcgtaaag gataagtcac atatttctgg aggggatatg attcttgagc
    36421 gcgttgaaat tgtgggtttt cgcggtatca accgtttgtc gttgatgctg gaacaaaaca
    36481 acgtcctgat tggggagaac gcgtggggta aatccagctt gctggacgcc ttaaccctgc
    36541 tgctatcgcc agaatcagat ctctaccatt ttgagcgcga cgatttctgg ttcccgccgg
    36601 gagatatcaa cgggcgagaa catcatctgc atattatttt gaccttccgc gaatcgctgc
    36661 caggccgaca tcgggttcgc cgttatcggc cgctggaagc gtgctggacg ccatgcaccg
    36721 atggctatca ccgtattttt tatcgtctgg aaggggagag tgcggaagac ggcagcgtga
    36781 tgacactgcg cagttttctc gataaagacg gacatccgat tgatgtcgag gatattaacg
    36841 atcaggcacg ccatctggtg cgtttaatgc cggtgctgcg cttgcgtgat gcccgtttta
    36901 tgcgccgtat tcgtaacggc acggtgccaa atgtccctaa tgtggaagtc accgcgcgcc
    36961 agctcgattt cctcgcccgt gagttatcct cacatccgca aaatctctct gatgggcaga
    37021 ttcgtcaggg actttccgca atggtacagc tgcttgagca ttatttctct gagcaggggg
    37081 ccggacaggc gcgatatcgt ttaatgcggc ggcgagccag caatgagcaa cgaagctggc
    37141 gctatctgga tatcatcaac cggatgattg accgacctgg tgggcgctcg tatcgggtta
    37201 ttttgctcgg cctgtttgct actttgttgc aggcaaaagg cacattgcga ctggataaag
    37261 acgcccgtcc attgttgctg atcgaagatc cagaaacccg tttacacccc attatgcttt
    37321 cagttgcctg gcatctgttg aatcttctgc cattgcagcg cattgccacc accaactcgg
    37381 gtgagttgct ttcgttaacg ccggtagagc atgtttgccg actggtacgt gagtcctcgc
    37441 gcgttgccgc ctggcgtctg gggccgagtg gcttgagtac cgaagatagc cgacgcatat
    37501 cctttcacat tcgttttaac cgtccgtcat cgctgtttgc acgctgctgg ttgctggtgg
    37561 aaggggaaac ggaaacctgg gttatcaatg aactggcgcg tcagtgcgga catcattttg
    37621 atgccgaagg gatcaaggtc attgagtttg cccagtccgg gctaaagcca ctggttaaat
    37681 ttgcccgccg aatggggatt gaatggcatg tactggtcga tggcgatgaa gcagggaaga
    37741 aatatgccgc tacggtacgc agcctgttga ataacgatcg ggaagccgaa cgagaacatt
    37801 taacggcgtt accggcgctg gatatggaac attttatgta tcgccaggga ttttccgatg
    37861 tgttccaccg cgtggcgcaa atcccggaaa atgtaccgat gaatctacgc aaaattatct
    37921 cgaaagcgat ccatcgctct tccaaacccg atcttgccat tgaagtggca atggaggcag
    37981 gacgtcgtgg tgtggactcc gtaccgacgc tgctgaaaaa aatgttctca cgcgtgctgt
    38041 ggctggcgcg cggtcgcgcg gattaaccgc gaaacatcgt ggccatttgt ggctgaatag
    38101 cgtcgagcat ctcatagcgc cgacggtatt cagcccgttt tttactggcg atttcggcaa
    38161 tctcttttcg tgctatctgt gctggaaggc ggtaatggcg ttcagcatca catacgccgc
    38221 caaccgattc ccagaaagcg ttgtaatcag cgtggatctt gccttcttta tcgcgataac
    38281 gcaggctgcg gtaaatatgc gtttcattgc tgacggcaat aatctgctct acctgcaaac
    38341 gttgggcaaa cagacaggcc gcttccatca cgaggcgttt ggcaaatagc ccgtggcagg
    38401 ctttcgtcgc attctggatt tcctgatgtg gaatttccca ttttgcgcct tgcagtccgc
    38461 caataaacat cgttcttttc ccctgatatt cacacagggt aaacgtgatc tctgccagag
    38521 gaataccttc gctgttgcgg aacaggattg tgctgtcacc ttctttatcc attgagatca
    38581 tcatggtcag ctcaagcgtg aactgctcgc cgtttttgcc ttccagcttc gccagttgca
    38641 gcccgggggt attcaaatat aagctgaatt cttccgccga catacatccg cggagtaacg
    38701 cataatggta acgtaacgcc tccagcaatt gcttacggct aagattcgcc gcaaggtaag
    38761 ggcgatgcag acgcacaggc agtcgcggct ggcgcgttaa caatacattg agattaggcc
    38821 agtgggaaag ttcgttcatc cactcaacgc ttaaacgcgg cataatcaac gagcgcagca
    38881 aaaatttctg gcgaaaacta cggcgatgcc agaatttacc cggccgacac tgtccacgtg
    38941 ccagactaag aaaaagtgac aggctgctga gagattcaga tggcgtaaag gtccgttcag
    39001 ttagctgcga catattcatg aaatcaatgg ttatacatga cgtcgatttc accattgcgt
    39061 atcttaacca aacatcaata gtgtgattac taacgtaaat tttaggggtt tgttgatatt
    39121 tcgttgaagt taatgacccg gattggcata tggagtattc agaatattta tgaaaaagcg
    39181 gaaaaccgtg aagaagcgtt acgttattgc gctggtgata gtcatcgccg gactgattac
    39241 gttatggaga attcttaacg cacccgtgcc gacttatcag acactgattg tgcgccccgg
    39301 tgatttacag caaagcgtgc tggcgaccgg aaagctggac gcgctgcgta aggttgacgt
    39361 gggcgcgcag gtcagcggtc agttgaaaac gctgtcggtg gcgattggcg ataaagtaaa
    39421 aaaagaccag cttttagggg tcattgatcc tgaacaggct gaaaaccaga tcaaggaggt
    39481 cgaagcaacg ctgatggagc tacgcgcgca gcggcagcag gcggaagcgg agctgaaact
    39541 ggcgcgggtg acgtattccc gtcagcaacg tctggcacaa acgcaggcgg tttcacagca
    39601 ggatctcgac accgccgcga cggagatggc tgtgaaacag gcgcaaattg gcaccattga
    39661 cgcgcaaatc aagcgcaatc aggcttctct cgatacggct aaaaccaatc tcgattacac
    39721 tcgcatcgtt gccccgatgg ccggggaagt cacgcaaatc accactctgc aaggccagac
    39781 ggtgattgcc gcacaacaag caccgaacat tctgacgctg gcagatatga gcaccatgct
    39841 ggtaaaagcg caggtttctg aagcggatgt aatccacctg aagccggggc aaaaagcctg
    39901 gtttacggta cttggcgatc cactgacgcg ctacgagggg caaatcaagg atgtactacc
    39961 gacgccggaa aaggttaacg acgctatttt ctattacgcc cgttttgaag tccccaaccc
    40021 caatggtttg ctgcggctgg atatgactgc gcaagtgcat attcagctca ccgatgtgaa
    40081 aaatgtgctg acgatccctc tgtcggcgtt aggcgatccg gttggcgata atcgttataa
    40141 agtcaaattg ttgcgtaatg gtgaaacacg cgagcgtgaa gtgacgattg gcgcacgtaa
    40201 cgataccgat gttgagattg tcaaagggct ggaagcgggc gatgaagtgg tgattggtga
    40261 ggccaaacca ggagctgcac aatgacgcct ttgctcgaat taaaggatat tcgtcgcagc
    40321 tatcctgccg gtgatgagca ggttgaggtg ttgaagggca tcaccctcga tatttatgct
    40381 ggtgagatgg tggcgattgt tggcgcttcg ggttccggta aatcgaccct gatgaatatt
    40441 ctcggctgtc tggataaggc caccagcggc acctatcgcg tcgccggtca ggatgttgcc
    40501 acgctggacg ccgatgcgct ggcgcaactg cgccgcgagc atttcggctt tattttccag
    40561 cgttaccatt tgctttcgca tttaaccgcc gagcagaacg ttgaagttcc cgccgtctat
    40621 gctggtcttg agcggaaaca gcggctgctt cgcgcccagg agttgctgca acggctgggg
    40681 ctggaagacc gtacagagta ttatccggca cagctttcgg gtggtcagca acagcgcgtc
    40741 agcatcgcgc gggcattgat gaacggcggt caggtaattc ttgccgatga accaaccggc
    40801 gcactggaca gccattctgg cgaagaggtg atggcgatcc tgcatcagct gcgcgatcgc
    40861 gggcatacgg tgattatcgt cacccacgat ccgcaggtcg ctgctcaggc cgagcgggtg
    40921 atcgaaattc gcgacggcga aattgtgcgc aatcctcccg ccattgaaaa agtgaatgtt
    40981 gctggcggga cggagcctgt tgtcaacacg gtgtctggct ggcggcagtt tgtcagcggt
    41041 tttaacgagg cgctgacgat ggcatggcgg gcgctggcag cgaataaaat gcgtacttta
    41101 ctgaccatgc tggggattat tatcggtatt gcgtcggtgg tttccattgt cgtggtgggt
    41161 gacgccgcca aacaaatggt gctggcggat attcgttcta ttggtacgaa tactattgat
    41221 gtctatcccg ggaaagattt tggcgatgac gatccgcaat atcaacaggc gctgaagtac
    41281 gacgacttaa tcgccatcca aaaacaaccg tgggtcgcct cagccacacc tgccgtctcg
    41341 caaaacctgc gcctgcgtta taacaatgtt gatgttgctg ccagtgccaa tggcgtgagc
    41401 ggcgattatt ttaatgtcta tggcatgacc ttcagtgaag ggaacacctt taatcaggag
    41461 cagctgaacg gtcgtgcgca ggtcgtggtt ctcgacagta atactcgccg ccagcttttc
    41521 ccccataaag cagatgtggt tggcgaggtg attctggtcg gcaatatgcc cgccagagtc
    41581 attggtgtgg cggaagaaaa acagtcgatg tttggtagca gtaaagtgct gcgtgtctgg
    41641 ctaccttaca gcacgatgtc cgggcgagtt atgggccagt cgtggcttaa ctccattact
    41701 gtcagggtga aagaaggatt tgacagcgcc gaggcggaac agcaactcac gcgtttactt
    41761 tcactgcgcc acggaaagaa ggatttcttt acctggaaca tggacggcgt cttgaaaact
    41821 gttgaaaaga ccacacgtac tttacaactg tttctgacgc tggtggcggt gatttcgctg
    41881 gtggtgggcg gtattggtgt aatgaatatt atgctggtgt cagtgaccga gcggacgcgg
    41941 gaaattggca ttcgcatggc tgtaggtgcg cgagcaagcg atgttttgca acagttcctg
    42001 atcgaagccg tactggtttg cctggtcggt ggcgcgttgg gaataacact gtcactgtta
    42061 attgctttca ccttgcagct tttcttaccc ggctgggaga ttggtttttc accgttggcg
    42121 ctgctgctgg cgtttctctg ctcgacggtc accgggattt tatttggctg gttacccgca
    42181 cgaaatgcgg cacgactgga tccagtagat gctctggcac gagagtaatt tttgagataa
    42241 aaatgccagc cgatcgggct ggcattttgc ctttaggatg tacacaatga gacagaagag
    42301 ctatgcgact gccgcttcta cttcgacggg cacaataaca ctggcgtgat tgccttttgg
    42361 cccctggtgg acatcaaact gaacggattg tccagctttt agcgttctgt aaccatccat
    42421 ctgaatggtg gaataatgag cgaaaatatc ttcgccgccg ccttcagggc agatgaaacc
    42481 aaaccctttg gcattgttga accacttaac agtacccttt tccatgcttc gacatccttc
    42541 gcaaatctta tacaagtaag atggaataaa ccggggtcag agagggggct gttcaaaacc
    42601 tcgccaactc tagaaataca atttagagaa ttagggcgag ccgtcaagca tttgacaggg
    42661 gacagggggc aggtatgaat caaaaatttg aagcagttaa cgctattgac aggaatgtga
    42721 cagatgtcgc tgatgccaac gatagatgat agttatctat catgtggagt agattggtca
    42781 ggcaaataag ctcttgtcag cggcagggcg ttctgccgat aaccgtaacc gaagatgata
    42841 actgacaatg ggtaaaacga acgactggct ggactttgat caactggcgg aagaaaaagt
    42901 tcgcgacgcg ctaaaaccgc catctatgta taaagtgata ttagtcaatg atgattacac
    42961 tccgatggag tttgttattg acgtgttaca aaaattcttt tcttatgatg tagaacgtgc
    43021 aacgcaattg atgctcgctg ttcactacca ggggaaggcc atttgcggag tctttaccgc
    43081 cgaggttgca gaaaccaaag tggcgatggt gaacaagtac gcgagggaga atgagcatcc
    43141 attgctgtgt acgctagaaa aagcctgaat gcaggcataa aaattggggg aggtgcctat
    43201 gctcaatcaa gaactggaac tcagtttaaa tatggctttc gccagagcgc gcgagcaccg
    43261 tcatgagttt atgaccgtcg agcacttgtt actggcgctg ctcagtaacc catctgcccg
    43321 ggaggcgctg gaagcgtgtt ctgtggattt ggttgcgctc cgtcaggaac tggaagcctt
    43381 tattgaacaa accacacccg ttctgcctgc cagtgaagag gagcgcgaca cacagccgac
    43441 gctgagtttt cagcgtgtac tgcaacgtgc ggtcttccat gtccagtcct ccggtcgcaa
    43501 tgaggttacc ggtgcaaacg ttctggtcgc tatctttagc gaacaggagt cgcaggcggc
    43561 atatctgttg cgtaaacatg aagtcagccg tctcgatgtg gtgaatttta tctctcatgg
    43621 cacgcgtaaa gacgagccga cacagtcttc tgatcctggc agccagccaa acagcgaaga
    43681 acaagctggt ggggaggaac gtatggagaa tttcacgacg aacctgaatc agcttgcgcg
    43741 cgtgggcgga atcgacccac tgattggtcg tgagaaggag cttgagcgtg ctattcaggt
    43801 tctctgccgt cgccgtaaaa acaacccgct gctggtgggg gaatctggtg tcggtaaaac
    43861 cgcgattgca gaaggtcttg cctggcgaat tgttcagggc gatgtgccgg aagtgatggc
    43921 tgactgtacg atttactctc tcgatatcgg ttctctgtta gcgggcacaa aatatcgcgg
    43981 cgactttgaa aaacgtttta aagcgttgct caagcagctg gagcaggaca ctaacagcat
    44041 cctgtttatt gatgagatcc acaccattat cggtgcgggt gcagcgtctg gtggtcaggt
    44101 cgatgcggct aacctaatca aaccgttgct ctccagcggt aaaattcgcg taattggttc
    44161 gacaacctat caggagttca gcaacatttt cgagaaagac cgtgctctgg cgcgtcgctt
    44221 ccagaaaatt gatattactg aaccgtcgat cgaagaaact gttcaaatca tcaatggcct
    44281 gaaaccgaag tatgaagcgc accacgacgt gcgttatacc gcaaaagcgg tgcgtgcggc
    44341 ggtagagctg gcggtgaaat acattaacga tcgtcatctg ccggataaag ccattgatgt
    44401 tatcgacgaa gcgggcgctc gcgcacgcct gatgccggta agcaaacgca agaaaaccgt
    44461 taatgtggcg gatattgagt ccgtggtggc ccgtattgca cgcattccag agaagagtgt
    44521 ttctcagagt gatcgtgata ccctgaaaaa cctcggcgat cgcttgaaaa tgctggtctt
    44581 cggtcaggat aaagccattg aggcgctgac tgaagccatt aagatggcgc gtgcaggttt
    44641 aggtcacgaa cataaacctg tcggttcgtt cctgtttgcc ggccctaccg gggtcgggaa
    44701 aacagaggtg acggtacagc tttcgaaagc tttgggcatt gagcttctgc gctttgatat
    44761 gtccgagtat atggaacgcc ataccgtcag ccgtctgatt ggtgcgcctc cgggatacgt
    44821 tggttttgat cagggcggtt tgctgaccga tgcggtaatc aagcatccac atgcggtgct
    44881 gctgctggac gaaatcgaga aagcgcatcc ggacgtgttc aatattctgt tgcaggtgat
    44941 ggacaacggt acgctgaccg ataacaacgg acgcaaagcg gacttccgta acgtggtgct
    45001 ggtgatgacc actaacgccg gggtacggga aactgagcgc aaatccattg gtcttatcca
    45061 ccaggataac agcaccgatg cgatggaaga gatcaagaag atctttacac cggaattccg
    45121 taaccgtctc gacaacatta tctggttcga tcatctgtca actgacgtga tccatcaggt
    45181 ggtggataaa ttcatcgtcg agttgcaggt tcagctggat cagaaaggtg tttctctgga
    45241 agtgagccag gaagcgcgta actggctggc cgagaaaggt tacgaccggg caatgggcgc
    45301 tcgtccgatg gcgcgtgtca tccaggacaa cctgaaaaaa ccgcttgcca acgaactgct
    45361 gtttggttcg ctggtggacg gcggtcaggt caccgtcgcg ctggataaag agaaaaatga
    45421 gctgacttac ggatttcaga gtgcacaaaa gcacaaggcg gaagcagcgc attaatctga
    45481 ttgtcaggta ggttggtgaa gtccgtaatc tcgaaagagg ttacggactt tttatttatg
    45541 gggtggagga ggttcagacc ctttttttga tgatgatggt aagttgttga taattagtgc
    45601 tgcggtaagg taaggataaa aaaagggtgg tagcaggaaa atgagatggt tttgctttat
    45661 taacaacggg ctaaacgtgt agtatttgtg ttcactgccg tacaggcagc ttagaaagct
    45721 gtattcggca aatgaagtgg atgttgatgg ttcactgcca tacaggcagc ttagaaattc
    45781 acaggtaaca tactccaccc gcccaccatg ttcactgccg tacagacaga taaaatgcga
    45841 aaaaaaaagc tcgcactttc gtacgagctc ttctttaaat atggcggtga ggggggaatg
    45901 actcgcttcg ctcgccctgc gggcagccca ctcactgcgt tcgtggtctg tccaactggc
    45961 tgcgccagtt gtcgaacccc ggtcggggct tctcaccccc ctagagtgca ttatgcgaaa
    46021 aaaaagctcg cactttcgta cgagctcttc tttaaatatg gcggtgaggg ggggattcga
    46081 acccccgata cgttgccgta tacacacttt ccaggcgtgc tccttcagcc actcggacac
    46141 ctcaccaaat tgttttgcta ccaaacctca tgggtggcaa cggggcgcta ctatagggag
    46201 ttggagtaaa acggtcaaga agaattttaa tgataattat tgtttgctca tactgtaaac
    46261 aagttgtgca gtatatctac atcgagacaa gttacggact tatacttcca aagtacttca
    46321 tacatatcac aaaataaaaa ggccggttaa accgaccttt tactcgttct ttctcttcgc
    46381 ccatcaggcg gtaaaacaat cagcgactac ggaagacaat gcggcctttg ctcaggtcgt
    46441 acggggtcag ttcaacagtc actttgtcgc ccgtcaggat gcggatgtag tttttgcgca
    46501 ttttaccgga gatgtgtgca gtaaccacgt gaccgttttc taactctacg cggaacatgg
    46561 tattaggcaa cgtttcaaga acggtacctt gcatttcaat attgtcttct ttggccatct
    46621 aatcctctgg ggtatcacta ccgtaatttg aaccggcaag ataatgccga agttctgtaa
    46681 ataagtaaag atttgcgcgc taaatcgcaa caaacaggtt cggcacatta ccccgaaaac
    46741 acacggctaa gccgcaccaa aagcgcaacg tataagggag cggtgagata aacgatgggc
    46801 gttacctgac gcgaaaaatt ccttatcggc agcggggtaa tgagcgtaac caactctgcg
    46861 accgcaatta taacactctg gggagaaatg tgccgaaaac attcattctt gtggtgaaaa
    46921 caagcatcgt ggtacccaga aattattcgg caatcgtccg aggcgcattt gattgagata
    46981 attcagataa tcccgacggg gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag
    47041 gacctggcag tcgataagct taccgccatg accgataaat tcctcacaga ataccagaag
    47101 cgccgtttta gacgcatttt ccatccggct aaacatggac tcgccacaaa atagcgttcc
    47161 ctgggccacg ccgtacatac cgccgacaag ctcatcttca cgccagactt caatggagtg
    47221 ggcatggccg agttcgtgaa ggcgatggta ggcttcgacc acgccacgcg tgatccaggt
    47281 tccttcttcg cgatcgctgg cacagccttc aatgacctga ccaaaagcgt aattcatcgt
    47341 gacacgatag ggcgagcgtt tatgaaatcg cttcatacta cggctgatat gcagtgattc
    47401 tggccatagc accgcgcggg gatcgggcga ccaccagagg atggggtcgc ctggagaaaa
    47461 ccacggaaaa ataccacgct ggtaagccat taacaggcgc gcagggctaa gatcgccccc
    47521 aagtgccagc aggccgttag gctcacgtaa tgcgccttcc ggggaaggga aggctattga
    47581 atggcgagaa agctgaacca ggcgcatgac cgcaaaactc cacgcaagtc ggatcgttca
    47641 ataatagctt acaaaccctg cttgaactgg taataacgcc cctgtctggc aagcagctct
    47701 gcgtgagtac cttgctcaat aatttgcccg ttgtccatca ctattatttg ttggaaacga
    47761 gagagtccgc gaagtcgatg ggtgaccatt aacaccgttt tctcacgcat catttctgca
    47821 agcaattcaa ggatctggct ttcggttgtg gcatctaagc cttcggtagg ttcatccagc
    47881 aacaccagtg gcgcatcatg taacagcgca cgggcgatag ccagacggcg cagttcacca
    47941 ccggagagct ggcgtccgcc ttcacctaac caactgttga gacctgcatc ctcgagcagc
    48001 ttctccagac caacgcgacg caagatcacc gccagagcct catcactact gccaggcgag
    48061 gcgagtaaca gattatcacg cagcgtggcg ctaaacagat gcactcgttg aggaacaacg
    48121 ctgatggtct gtcgtagagc cgcttcattc aggctggcta tggggctatc gttaagcaaa
    48181 atctcgccct gttgcgggtc ccaagcgcgg gtcagcagtt gtaacagtgt tgatttgccg
    48241 catccggttc gcccgagaat cgcaatatgt tccccggcgt ttacctgaag agaaatccct
    48301 ttaagtgcct gttgggattg ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa
    48361 acgcgatcgg caacacgagt ttgggtatca gggaaggtga cttccggttt ttgatccgtt
    48421 aaatcagtga tccgaacggc agaggcaatg acttgcccca gatgctgaaa tgcacccgtt
    48481 actggtgcca gtgcttcaaa cgcggctaac gcgcagaaga caaacagggc aattaacgcg
    48541 ccgggttgag cattgccgcc aacgccgcca gacgccatcc acagcatcag gatcaccgct
    48601 aacgcgccaa tgagcagcat tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt
    48661 tgcgcttcca gccattgaat ttctgtattc tctagttgcg tgcgataacg atcgctggca
    48721 ccaaaaatgg tcagctcagc ttgcccttgc agccaggccg tcagttgttg gcgatactgt
    48781 ccgcgaagat gagtcagatt ttgcccggtg ctttttcccg cacgataaaa cagcggtggc
    48841 atcaggaaaa gcgtcagtaa cataatgctg cccagcgtaa aggcgagggt gaaatcaagg
    48901 aaacttaacc cgattgtcac caccataatc accacaaaag cgcccaccag cggcgagata
    48961 acgcgcaggt aaagatgatc gagcgtatca acatccgcca ccacgcggtt gagcaattcg
    49021 ccctgacgat agcgcgccag tccggcaggg gagaggggca acaatttgct gaaggtgtaa
    49081 atgcgcagat gctgcaacac gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa
    49141 cgcccggcag tacgggtgat tgcggcacca cgcacgcccg cagcgggtag catatagttg
    49201 aagctgtaca gtccggcaac ccccgcaacc gctgaggccg agaggaacca gccggaaagt
    49261 gtcaacagac cgatactggc gagcagcgtc acaattgcca gcacaatacc aagacttaac
    49321 atccatttat gacgtttata cagtgccaga tagggtagca aagcgcgcat ttaaatctcc
    49381 tcctgacgat gggccagtaa tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa
    49441 cgtccttgct caataatccg gccatcctgc ataacccaaa tgacatccca gtcagcaaga
    49501 tcttctaact ggtgggtgac cattaacgtt gtctggcgca gagaggcggc attcagcgcc
    49561 tccattacgc gctgttcact gtgagcatca aggctggcag cgggttcatc caacagtaat
    49621 agcgaacagg gatttagtaa cgcacgggcc accgccacgc gctgcgcctg ccccacggaa
    49681 aggcgggcag cctggtcgcc aacaggcgta tcaacgcctt gtggcaggag cggtagaaac
    49741 tcgctgaccc aggcgttatc cagcgctgct tgtaattcat gttcgctggc atcaggtcgc
    49801 gccagtagta cgttatcccg taatgttgct gccggtaatt gtggattttg cccaacccag
    49861 gagagatgtt tacgccatga ttctggtgat aaatcgcgta attctatccc gttgattcgt
    49921 agcgatccct gatatgagag aaaaccagaa agcgcgttca acagtgaact tttacctgaa
    49981 ccgctgcgac caaccaacac ggcacgttgg cctgctggca aagtaaagtt cagcggtccg
    50041 gccagcgttt taccttccgg cgacgtgata aacagatcct cggcctcaat ggtcaccgga
    50101 tcggtcaatg ccaattccgc ctcaccgcgc tgcggatggg cgagcggggt ttccataaac
    50161 gttttcagac tgtcagctgc accaacagcc tgggctttag catgataaaa cgtaccgaga
    50221 tcgcgtaatg gctggaaaaa ctctggcgca aggatcaggg ccagaaaacc cgcagccagc
    50281 gtcacgccag tatcgtagtg accaaaatcc agctcgccga gataggaaaa accaaagtag
    50341 accgccacca gagcaattga cagcgaggta aaaaattcga gaatgccgga ggataaaaat
    50401 gccagccgta gcacttccat tgtccgttgg cggaaatctt ccgaagcaga acgaatactt
    50461 tcaatttcag cttcaccacg accaaaaata cgcaatgttt ccatgccgcg caggcgatcg
    50521 aggaaatgcc cacttaagcg agcaagagcg agaaagttac gtcggttagc atcggcagcc
    50581 cccattccaa ccagcgccat aaacaacgga attaacggtg cagtgcccag cagaatgagc
    50641 gccgcagccc agtttgaggg gaagattgcc accacaatca gcaacggcac cgacactgcc
    50701 agcgccattt gcggcaggta gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt
    50761 accagcgtcg cccagctccc cgcaggttta ccctgaatcc acgctggccc tgcttgttgc
    50821 agacggtcga gaacctgccg gcggatggca aagcggatat gctgcccggc gtgataaccc
    50881 acccgttcgc gtaaccagac cacccatgcg cgcagtacaa aggtcagaac cagtaacgta
    50941 aagggaagca gcagggcttc acggggaata ttctccataa tcatatgttg cagaattcgc
    51001 gccatgaacc aggcctgggc aatgatcaat atgccgctca caaagcccag cagacgagaa
    51061 atattcagcc aacgttggga gatgacgctt tgctgtttta accagcgggt taactctttt
    51121 tgacgagatt tattcattgc acgcttagca ggtgagttat cagaattatt tgcagagcaa
    51181 tgttacaacg gggaaaaaat aaaggcgacc catagtcgca tggtgtcgcc ttctttactt
    51241 ttgttactga tttgtaaaat tattttgcgt cagctaaacc atcgaggtag cgttccgcat
    51301 caagtgctgc catgcagcct gtaccagcag atgtaatggc ctggcgataa atgtgatcca
    51361 tcacgtcgcc tgcggcaaag acgccaggaa tgctggtctg ggtggcatta ccatgaatac
    51421 ccgactgcac tttgatgtag ccgttttcca gttccagctg cccttcgaaa atggcagtat
    51481 tcgggctgtg accgatagca acaaacagac cggcaacgtc gagtgactcg atgttatcgc
    51541 tgttttgcgt atcgcgcaga cgaacgccag tgacacccat ttgatcgccg gtcacttctt
    51601 ccagcgtacg gttggtgtgc agaatgatgt tgccgttctc cactttatcc atcagacgct
    51661 taataaggat tttttccgcg cggaaaccgt cacggcggtg aatcagatgc acttccgaag
    51721 cgatgttaga cagatacagc gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa
    51781 ctttctggtt gcgatagaag aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt
    51841 taaaggcttc ttcagagggc aggccgagat agcgtgcaga agctccggtg gcaataatca
    51901 gcgcgtcgca agtgtattcg ccgttatcgc cattcagacg gaacggacgg ttttgcagat
    51961 ccaccttgtt gatatgatca aaaatgatct cagtttcaaa cttggtggca tggtcgtgca
    52021 tgcgctccat taataacgga ccggtcagat cgtttggatc gccaggccag ttttccactt
    52081 ccgtggtggt ggtcagttgg ccgccttttt ccatgccggt aatcagcaca ggttgcaggt
    52141 tggcgcgcgc cgcgtagaca gcagcggtgt atcccgccgg gcctgaaccc aggataagca
    52201 gtttactgtg tttggtcgtg cccatgagat ccccatagtt gttggcagac aatgggcagg
    52261 attgtaggga atttacagac gtaaaaaaag agtatgacga ttttgttaac aatttgtgca
    52321 atcggcagca tcgataagca ggtcaaattc tcccgtcatt atcacctctg ctacttaaat
    52381 ttcccgcttt ataagccgat taaatgatga ataaacgccc ctgttaatga atatctggca
    52441 tgttgtacta aaaatcgatg ttttgctttg acaatcccct ggtgttttgc gaaaacattc
    52501 gaggaagaaa aaaacagtat tcttatatgc gcataaccat gcatgtaaat accatgttta
    52561 ccgtgctagt gaaatctacg tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc
    52621 cacaaggcaa cggtcttctc accgtagacc caggcattgc gcgccgtgaa tcttcatgat
    52681 ttcggtctat cgtgacgggt agcgactctg aacagtgatg tttcagggtc agacaggagt
    52741 agggaaggaa tacagagaga caataataat ggtagatagc aagaagcgcc ctggcaaaga
    52801 tctcgaccgt atcgatcgta acattcttaa tgagttgcaa aaggatgggc gtatttctaa
    52861 cgtcgagctt tctaaacgtg tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg
    52921 gctggaaaga caagggttta ttcagggcta tacggcgctg ctgaaccccc attatctgga
    52981 tgcatcactt ctggtatttg ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga
    53041 acaattcaat accgctgtac aaaaacttga agaaattcag gagtgtcatt tagtatccgg
    53101 tgatttcgac tacctgttga aaacacgcgt gccggatatg tcagcttacc gtaagttgct
    53161 gggggaaacc ctgctgcgtc tgcctggcgt caatgacaca cggacatacg ttgttatgga
    53221 agaagtcaag cagagtaatc gtctggttat taagacgcgc taacacggaa caggtgcaaa
    53281 atcggcgtat tttgattaca ctcctgttaa tccatacagc aacagtactg gggtaacctg
    53341 gtactgttgt ccgttttagc atcgggcagg aaaagcctgt aacctggaga gcctttcttg
    53401 agccaggaat acactgaaga caaagaagtc acattgacaa agttaagtag cggccgccgc
    53461 cttctggagg cgttgctgat ccttattgtc ctgtttgccg tctggttgat ggctgcctta
    53521 ctaagcttta acccttcgga ccccagctgg tcgcaaacgg cctggcatga acctatccat
    53581 aatttaggtg ggatgcccgg tgcgtggctg gcagatacgc tgttctttat ttttggcgtg
    53641 atggcttaca ccattcccgt cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag
    53701 tccagtgacg aatacattga ttattttgcc gtttcgctac gcatcattgg cgttttggcg
    53761 ctcatcctta cctcctgtgg tctggcggca atcaacgctg acgatatctg gtattttgcc
    53821 tccggtggcg tcattggcag cttactaagc actacgctac aaccactgct acacagtagc
    53881 gggggaacta ttgcgctgct ctgcgtttgg gctgcgggcc tgacgctgtt caccggttgg
    53941 tcatgggtga ccattgctga aaaactcggc ggctggattt taaacattct caccttcgcc
    54001 agtaatcgta cccgtcgcga tgatacctgg gtcgatgaag atgaatatga agatgacgaa
    54061 gagtatgaag atgaaaacca cggcaaacag catgaatcac gccgtgcccg tattcttcgc
    54121 ggcgcgctag cgcgtcgtaa acggttggcg gaaaaattca ttaatccgat ggggcggcaa
    54181 acagacgctg cgttgttctc cggtaagcgg atggatgatg acgaagagat tacctacact
    54241 gcacgcggtg tggctgccga cccggacgac gtcctatttt cgggcaatcg tgcaacgcag
    54301 ccagaatatg acgaatacga tccattctta aacggtgcgc caattaccga acctgtcgct
    54361 gtagcagctg ctgctaccac ggcgacacaa agctgggctg cgccggttga acctgtgact
    54421 cagacgccgc ctgttgcctc tgttgatgtt ccaccttcgc aacctacagt agcctggcag
    54481 cctgtaccgg gtccacaaac gggagagccg gttattgctc ctgcaccgga aggttaccca
    54541 cagcagtcac aatatgcgca gcctgcagtg caatataatg agccgctgca acaaccagta
    54601 cagccgcagc agccgtatta tgcacctgca gctgaacaac ctgcgcaaca accttactac
    54661 gctcctgcgg cggaacaacc tgttcagcaa ccgtattacg caactgcgcc ggaacagcct
    54721 gcgcaacagc cgtattatgc ccctgcgcca gaacaaccgg tggcaggtaa cgcctggcaa
    54781 gccgaagagc agcaatccac ttttgctcca cagtctacat accagactga gcaaacttat
    54841 cagcagccag ccgctcagga gccgttgtac caacagccgc aacctgttga acagcagcct
    54901 gttgtggagc ctgaacccgt tgtagaagag acaaaacccg cgcgtccgcc gctttactac
    54961 tttgaagaag tggaagagaa gcgagcccgt gaacgtgaac aacttgcggc ctggtatcaa
    55021 ccgattccag aaccggttaa agaaccagaa ccgatcaaat cttcgctgaa agcaccttct
    55081 gttgcagcag tacctccagt agaaaccgct gccgctgttt ctccgctggc atctggcgtg
    55141 aaaaaagcga cactggcgac gggggctgcc gcaaccgttg ccgcgccagt cttcagtctg
    55201 gcaaatagcg gtggaccgcg tccgcaggtc aaagagggga ttggcccgca gttgccacga
    55261 ccgaaacgta tccgcgtgcc aactcgtcgt gaattggcgt cttacggtat taagctgccc
    55321 tcacagcgtg cggcggaaga aaaagcccgt gaagcccagc gcaatcagta cgattctggc
    55381 gatcagtaca acgatgatga aatcgatgcg atgcagcagg atgaactggc acgtcagttc
    55441 gcccagacac agcagcaacg ctatggcgaa cagtatcaac atgatgtgcc cgtaaacgca
    55501 gaagatgcag atgctgcggc agaggctgaa ctggctcgtc agtttgcgca aactcaacaa
    55561 caacgttatt ccggcgaaca accggctggg gcgaatccgt tctcgctgga tgattttgaa
    55621 ttttcgccaa tgaaagcgtt gctggatgat ggtccacacg aaccgttgtt tacgccaatt
    55681 gttgaacctg tacagcagcc gcaacaaccg gttgcaccgc agcagcaata tcagcagccg
    55741 caacaaccag ttccgccgca gccgcagtat cagcagccac aacagccggt tgcgccgcag
    55801 ccacaatatc agcagccgca acaaccggtt gcgccacagc agcaatatca gcagccgcaa
    55861 caaccggttg cgccgcagca gcagtatcag cagccacaac agccagttgc gccacaaccg
    55921 caggataccc tgcttcatcc gctgttgatg cgtaatggcg acagccgtcc gttgcataaa
    55981 ccgacgacgc cgctgccttc tctggatttg ctgacaccgc cgccgagcga agtggagccg
    56041 gtagatacct ttgcgcttga acaaatggct cgcctggtgg aagcgcgtct ggctgatttc
    56101 cgtattaaag ccgatgtcgt caattactct ccggggccgg ttatcactcg ctttgaactt
    56161 aacctggctc ctggcgtaaa agcggcgcgc atttctaact tgtcacggga ccttgcccgt
    56221 tcactttcga cggtggcggt gcgtgtcgtt gaagttattc ctggcaaacc ctatgtaggt
    56281 ctggagttac caaataaaaa acgacaaacc gtttatctgc gcgaagtttt ggataacgcc
    56341 aaattccgcg ataatccgtc gccattaacc gtggtgctgg gtaaagatat cgccggtgag
    56401 ccggtggttg ccgatctggc gaaaatgccg cacttgttgg ttgcggggac taccggttcc
    56461 ggtaaatctg tcggtgtgaa cgcgatgatc ctgagcatgc tttataaagc acagccagaa
    56521 gatgtgcgtt tcatcatgat cgacccgaaa atgctggagc tttcggttta tgaaggcatt
    56581 ccgcatctgt taacggaagt cgttactgat atgaaagatg ccgccaacgc gctgcgctgg
    56641 tgtgttaacg agatggagcg tcgttataaa ctgatgtctg cgctgggtgt gcgtaatctg
    56701 gcgggttata acgaaaaaat tgctgaagcc gatcgcatga tgcgtccgat tccagacccg
    56761 tactggaagc cgggtgacag tatggatgcc cagcatccgg tgctgaaaaa agaaccatac
    56821 attgtggtgt tggttgacga atttgccgac ctgatgatga cggtaggtaa aaaagtggaa
    56881 gagctgatag cacgtctggc gcaaaaagcc cgtgccgcgg gtatccacct cgtactggca
    56941 actcagcgtc catcggttga tgttattact ggtctgatta aagcgaatat tccgacccgt
    57001 atcgccttta ccgtatccag taagattgac tcacgtacca ttcttgatca ggctggcgcg
    57061 gaatcactgc tggggatggg ggatatgctc tactctgggc cgaactccac gttgccggta
    57121 cgtgtccatg gtgcttttgt tcgcgatcag gaagttcatg ccgtggtgca ggactggaaa
    57181 gcgcgtggtc gcccacagta tgttgatggc atcacttccg acagtgaaag cgaaggtggc
    57241 gcgggcggtt tcgatggcgc tgaagaactg gatccgttgt tcgatcaggc ggtgcagttt
    57301 gtcactgaaa aacgcaaagc gtcaatttct ggcgtacagc gtcagttccg cattggttat
    57361 aaccgtgcag cgcgtattat cgaacagatg gaagcgcagg ggattgtcag cgaacagggg
    57421 cacaacggta atcgtgaagt gctggcccca ccgccgtttg actaattaat gcattgccgg
    57481 ataaggcgcg gtagcgtcgc atccggcact ctatcaactg aaaattcagt attttcttct
    57541 ttcctcaagc tgattattag cctggaatag agagtagagg gaactcccga tcgggagtga
    57601 cgtaatttga ggaataatga tgaaaaaaat tgccatcacc tgtgcattac tctcaagctt
    57661 agtagcaagc agcgtttggg ctgatgccgc aagcgatctg aaaagccgcc tggataaagt
    57721 cagcagcttc cacgccagct tcacacaaaa agtgactgac ggtagcggcg cggcggtgca
    57781 ggaaggtcag ggcgatctgt gggtgaaacg tccaaactta ttcaactggc atatgacaca
    57841 acctgatgaa agcattctgg tttctgacgg taaaacactg tggttctata acccgttcgt
    57901 tgagcaagct acggcaacct ggctgaaaga tgccaccggt aatacgccgt ttatgctgat
    57961 tgcccgcaac cagtccagcg actggcagca gtacaatatc aaacagaatg gcgatgactt
    58021 tgtcctgacg ccgaaagcca gcaatggcaa tctgaagcag ttcaccatta acgtgggacg
    58081 tgatggcaca atccatcagt ttagcgcggt ggagcaggac gatcagcgca gcagttatca
    58141 actgaaatcc cagcaaaatg gggctgtgga tgcagcgaaa tttaccttca ccccgccgca
    58201 aggcgtcacg gtagatgatc aacgtaagta gaggcacctg agtgagcaat ctgtcgctcg
    58261 atttttcgga taatactttt caacctctgg ccgcgcgtat gcggccagaa aatttagcac
    58321 agtatatcgg ccagcaacat ttgctggctg cggggaagcc gttgccgcgc gctatcgaag
    58381 ccgggcattt acattctatg atcctctggg ggccgccggg taccggcaaa acaaccctcg
    58441 ctgaagtgat tgcccgctat gcgaacgctg atgtggaacg tatttctgcc gtcacctctg
    58501 gcgtgaaaga gattcgcgag gcgatcgagc gcgcccggca aaaccgcaat gcaggtcgcc
    58561 gcactattct ttttgttgac gaagtgcacc gattcaacaa aagccagcag gatgcatttc
    58621 tgccacatat tgaagacggc accatcactt ttattggcgc aaccactgaa aacccgtcgt
    58681 ttgagcttaa ttcggcactg ctttcccgtg cccgtgttta tctgttgaaa tccctgagta
    58741 cagaggatat tgagcaagta ctaactcagg cgatggaaga caaaacccgt ggctatggtg
    58801 gtcaggatat tgttctgcca gatgaaacac gacgtgccat tgcagaactg gtgaatggcg
    58861 acgcgcggcg ggcgttaaat acgctggaaa tgatggcgga tatggccgaa gtcgatgatc
    58921 gcggtaagcg ggtcctgaag cctgaattac tgaccgaaat cgccggtgaa cgtagcgccc
    58981 gctttgataa caaaggcgat cgcttttacg atctcatttc cgcactgcat aagtcggtac
    59041 gtggtagcgc acccgatgcg gcgctgtact ggtatgcgcg aattattacc gctggtggcg
    59101 atccgttata tgtcgcgcgt cgctgtttgg cgatagcgtc tgaagatgtg ggtaacgctg
    59161 atccacgagc gatgcaggtg gcaattgcgg cctgggattg ctttactcgc gttggcccgg
    59221 cggaaggtga acgcgccatt gctcaggcga ttgtttacct ggcctgcgcg ccaaaaagca
    59281 atgctgtcta cactgcgttt aaagccgcgc tggccgatgc tcgcgaacgc ccggattatg
    59341 acgtaccggt tcatttgcgt aatgcgccga cgaaattaat gaaggaaatg ggctatgggc
    59401 aggaatatcg ttacgctcat gatgaagcaa acgcttatgc tgccggtgag gtttacttcc
    59461 cgccggaaat agcacaaaca cgctattatt tcccgacaaa caggggcctt gaaggcaaga
    59521 ttggcgaaaa gctcgcctgg ctggctgaac aggatcaaaa tagccccata aaacgctacc
    59581 gttaatgtta tcgttgcggt aatgttgtta ctgtatccct gtggtcgcag gctgtggcca
    59641 catctcccat ttaattcgat aagcacagga taagcatgct cgatcccaat ctgctgcgta
    59701 atgagccaga cgcagtcgct gaaaaactgg cacgccgggg ctttaagctg gatgtagata
    59761 agctgggcgc tcttgaagag cgtcgtaaag tattgcaggt caaaacggaa aacctgcaag
    59821 cggagcgtaa ctcccgatcg aaatccattg gccaggcgaa agcgcgcggg gaagatatcg
    59881 agcctttacg tctggaagtg aacaaactgg gcgaagagct ggatgcagca aaagccgagc
    59941 tggatgcttt acaggctgaa attcgcgata tcgcgctgac catccctaac ctgcctgcag
    60001 atgaagtgcc ggtaggtaaa gacgaaaatg acaacgtcga agtcagccgc tggggcaccc
    60061 cgcgtgagtt tgactttgaa gttcgtgacc atgtgacgct gggtgaaatg cactctggcc
    60121 tcgactttgc agctgcagtt aagctgactg gttcccgctt tgtggtaatg aaagggcaga
    60181 ttgctcgcat gcaccgcgca ctgtcgcagt ttatgctgga tctgcatacc gaacagcatg
    60241 gctacagtga gaactatgtt ccgtacctgg ttaaccagga cacgctgtac ggtacgggtc
    60301 agctgccgaa atttgctggc gatctgtttc atactcgtcc gctggaagaa gaagcagaca
    60361 ccagtaacta tgcgctgatc ccaacggcag aagttccgct gactaacctg gtgcgcggtg
    60421 aaatcatcga tgaagatgat ctgccaatta agatgaccgc ccacacccca tgcttccgtt
    60481 ctgaagccgg ttcatatggt cgtgacaccc gtggtctgat ccgtatgcac cagttcgaca
    60541 aagttgaaat ggtgcagatc gtgcgcccgg aagactcaat ggcggcgctg gaagagatga
    60601 ctggtcatgc agaaaaagtc ctgcagttgt tgggcctgcc gtaccgtaaa atcatccttt
    60661 gcactggcga catgggcttt ggcgcttgca aaacttacga cctggaagta tggatcccgg
    60721 cacagaacac ctaccgtgag atctcttcct gctccaacgt ttgggatttc caggcgcgtc
    60781 gtatgcaggc acgctgccgc agcaagtcgg acaagaaaac ccgtctggtt cataccctga
    60841 acggttctgg actggctgtt ggtcgtacgc tggttgcagt aatggaaaac tatcagcagg
    60901 ctgatggtcg tattgaagta ccagaagttc tgcgtccgta tatgaacgga ctggaatata
    60961 ttggctaata cccaattttt ctgaatctaa aaagcgcctg cgggcgcttt ttttgtctcc
    61021 ctttgatacc gaacaataat tactcctcac ttacacgtaa tactactttc gagtgaaaat
    61081 ctacctatct ctttgatttt caaattattc gatgtataca agcctatata gcgagctgct
    61141 atagaaataa ttacacaata cggtttgtta ctggaatcaa tcgtgagcaa gcttgagtga
    61201 gccattttga aaacgaaaat ccctgatgcg gtattggctg ctgaggtgag tcgccgtggt
    61261 ttggtaaaaa cgacagcgat cggcggcctg gcaatggcca gcagcgcatt aacattacct
    61321 tttagtcgga ttgcgcacgc tgtcgatagc gccattccaa caaaatcaga cgaaaaggtt
    61381 atctggagcg cctgtacagt taactgtggt agtcgctgcc cgctacgtat gcacgtcgtg
    61441 gacggtgaaa tcaaatatgt cgaaacggac aataccggcg atgacaatta cgacggcctg
    61501 caccaggttc gcgcctgcct gcgtgggcgt tccatgcgtc gccgtgtcta caatccggac
    61561 cgcctgaaat atccgatgaa acgagtcggg gcgcgcggtg aaggcaaatt cgagcgcatt
    61621 agctgggaag aagcctacgg catcatcgcg accaatatgc agcgcctgat caaagagtac
    61681 ggcaacgagt ctatctatct gaactatggc accggtacgc tgggcggcac catgacccgc
    61741 tcctggccgc cgggaaatac cctggtcgcg cggctgatga actgctgcgg cggctatctg
    61801 aaccattacg gcgactactc ctccgcgcaa attgcggaag gtttgaacta tacctacggc
    61861 ggctgggcag atggcaacag cccgtcggat atcgaaaaca gtaagctggt agtgctgttt
    61921 ggtaataacc ctggcgaaac gcgaatgagt ggcggtgggg tgacttacta tcttgaacag
    61981 gcacgccaga aatctaatgc ccgcatgatc atcatcgatc cgcgctatac cgacaccggt
    62041 gccgggcgcg aagatgagtg gatccctatt cgtccgggaa cagatgccgc actggttaac
    62101 ggtctggcgt acgtcatgat cactgaaaac ctggtggatc aggcattcct cgataaatat
    62161 tgcgttggct acgatgagaa aaccctgcca gccagtgcgc cgaaaaatgg ccactataaa
    62221 gcttatattc tgggtgaagg gccagatggc gtggctaaaa cgccggaatg ggcctcgcaa
    62281 atcactggtg ttccggcaga caaaatcatc aaattggctc gtgaaatcgg tagtaccaaa
    62341 ccggcgttta tcagccaggg atggggcccg cagcgtcacg ctaacggtga aatcgcaacc
    62401 cgtgctatct cgatgctggc gattctgacc ggtaacgttg gtattaacgg aggcaacagc
    62461 ggcgcgcgtg aaggttcata cagcttaccg tttgtccgta tgccgacctt ggaaaacccg
    62521 atccagacca gcatttcgat gtttatgtgg accgatgcca ttgaacgtgg cccggaaatg
    62581 acggcgctgc gtgatggtgt acgcgggaaa gataagctgg atgtgccgat caaaatgatc
    62641 tggaactatg ccggtaactg cctgattaac cagcattctg aaatcaaccg tacccatgaa
    62701 atccttcagg atgataagaa gtgcgagctg attgtggtta tcgactgcca catgacctca
    62761 tcggcgaaat atgctgacat cctgctgcct gactgcaccg cttccgaaca gatggacttt
    62821 gcgctggatg catcctgcgg gaatatgtct tacgtgattt tcaacgatca ggtgattaaa
    62881 ccgcgctttg aatgtaagac catctatgaa atgaccagcg aactggcaaa acgtcttggc
    62941 gttgagcaac agtttactga aggccgtacc caggaagagt ggatgcggca tctgtatgcc
    63001 cagtcgcggg aagcgattcc tgaactgcca acgtttgaag agttccgcaa gcaggggatc
    63061 tttaaaaagc gcgacccaca agggcatcac gttgcttata aagccttccg tgaagatccg
    63121 caggcaaacc cactgactac gccatcgggc aaaattgaga tttattcgca ggcgctggct
    63181 gacattgccg ctacctggga attgcctgaa ggcgatgtga tcgatccact gccgatctac
    63241 acgccgggct ttgaaagtta tcaggatccg ctgaacaaac agtatccgct gcagcttaca
    63301 ggtttccact ataaatctcg cgttcactca acttacggca acgttgatgt gctgaaagcg
    63361 gcttgccgtc aggaaatgtg gatcaacccg cttgatgccc aaaaacgcgg tatccacaac
    63421 ggcgataaag tcaggatctt taacgatcgt ggtgaggttc atattgaggc gaaagtgacg
    63481 ccacgaatga tgccgggtgt ggtcgcactg ggtgaaggtg cctggtatga cccggatgca
    63541 aaacgtgtcg ataagggtgg ttgtattaac gtactgacca ctcaacgtcc gtctcctctc
    63601 gctaagggga atccgtcaca tacaaacctt gttcaggttg aaaaggtgta aggagtaacc
    63661 gatgacaacc cagtatggat tttttattga ttccagccgt tgcaccggtt gcaaaacctg
    63721 cgagctggct tgtaaagact acaaagattt gacgccagaa gtcagcttcc gccgcattta
    63781 tgaatatgct ggcggcgact ggcaggaaga taacggtgtc tggcaccaga acgtgtttgc
    63841 ctactatctg tcgatttcgt gcaaccactg cgaagatccg gcttgtacta aagtctgccc
    63901 gagcggtgcg atgcataaac gtgaagatgg ttttgttgtg gtcgatgaag atgtgtgcat
    63961 tggctgccgc tactgccata tggcttgccc gtatggcgca ccgcaatata acgaaacgaa
    64021 aggccatatg accaaatgcg atggttgtgt tatgaccgtg ttgcggaggg taaaaagccg
    64081 atctgtgttg aatcttgtcc gctgcgggcg ctggatttcg gccctatcga cgagctgcgt
    64141 aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg
    64201 aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg
    64261 gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt
    64321 cacggtcttc gggcaatgtg tagcaggtgg ttttatcgtt ctggctttgg cgctgctcaa
    64381 aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gtttatgggt
    64441 gctgatgggc attggcttta tcgcctctat gctccatctt ggttcaccaa tgcgcgcttt
    64501 taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat
    64561 cttttttgcc gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc
    64621 attgcgtacg ctgtggctga tagtgacgat ggttcttggc gtcatctttg tctggatgat
    64681 ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat
    64741 gggcttcttc ctgacgatgt ttatgggcgg cccgttactg ggttacctgt tgctgagtct
    64801 ggctggcgtc gatggctggg cgatgcgtct gctgccagca atttctgtac tggcactggt
    64861 agtgagtggc gtggtgtcag tgatgcaggg cgcagagctg gcgactattc atagttctgt
    64921 gcagcaggcc gcagcgctgg tgccggacta tggtgcgctg atgtcctggc ggatcgtgct
    64981 tttggccgtt gccctgtgct tgtggattgc accacagcta aaaggttatc agcctgcggt
    65041 tccgctactt tctgtatcat tcattctgct tctggcaggg gaattaatcg gtcgcggcgt
    65101 attctatggt ttgcatatga ccgtggggat ggccgtcgca agctaatcat aacaaccggg
    65161 gtttcggccc cggttctctt ttatttctgc ttcgttaacg tgtcataact ggtcatcaaa
    65221 ttacgataat caggaatgtg gttagagaac aacgtcgcca gtccttcaat atcattacgc
    65281 cagtcgcgat gcagctcgca cgccacgccg aaccatgtca tcaattgcgc accagcttgc
    65341 gacatgcgat cccatgccga atgccgggta atttcattaa aggtccctga cgcgtcagtc
    65401 acgacaaaga cgtcaaaccc ttcttcaatg gctgaaagcg ccgggaatgc cacgcaaact
    65461 tcggttacca caccggcaat aattaactgt tttttacctg tcgctttgac agcttttaca
    65521 aaatcttcgt tatcccaggc gttaatattt cccgggcgag caatgtaagg tgcatcgggg
    65581 aattgtgctt tcagttctgg aactaatggg ccgttaggac cggtttcaaa actggtggta
    65641 agaatagttg gtaagttgaa atacttagcc aggtcaccca gcgccagcac gttattttta
    65701 aacttatcgg gttcgatatc ccgtacaagg gaaagtaaac cagcctggtg atcaacaagc
    65761 aaaacggcag catcattttt atcaagacga acatacggtt tggtcatcct cttctccttt
    65821 cgagagtcag tggtacgtct gaaatcgatc agacgcgatg cattgctctg aaagcataga
    65881 cgggaaatat gagtttgctg tgaccatgaa atttttcgac tgaacgaagc gtatgaaatt
    65941 tgtgttagtt caataaaaac aatcagatga gactaatcat ctcttcagca ttagcgttat
    66001 gaatgagtaa tataaatcga taataccgcc gctgggatat cgcgtatttt cacccattga
    66061 caatgttttt ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc
    66121 atgtccacgt atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg
    66181 gcgattgcgg tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca
    66241 tggcaggtag gcgttgtcag ctcatcctat tttaccggca accttgccgg tacattgctg
    66301 acagggtatg tcattaagcg cattggcttt aaccgcagct attatctggc ctccttcatt
    66361 tttgccgctg gctgtgccgg ccttggcctg atgattggat tctggagctg gttggcttgg
    66421 cgttttgtcg cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg
    66481 tgcagtggga cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac
    66541 gtgggaacgt ttttaggcca gttactggtc agcaaagttt caaccgagct gatgtccgta
    66601 ttgccgtggg ttacaggttt gacgttggca gggatcttac cgctgttgtt tacgcgtgtg
    66661 ctgaatcagc aggctgaaaa ccatgattcg acgtcaatta cgtcaatgct aaaactccgt
    66721 caggcgcggc ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg atctctatat
    66781 ggcctgatgc cgctgtacct caatcacaaa ggggtgagca atgccagcat tggtttctgg
    66841 atggcggtac tggtcagtgc gggtatcctt ggacaatggc cgattggacg tctggcggat
    66901 aagtttggtc gactgctggt gttgcgtgtt caggtctttg tcgtcattct cggcagtatc
    66961 gcgatgctta gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt
    67021 acgctatatc cggtggcgat ggcatgggct tgcgagaaag ttgaacatca tcaactggtg
    67081 gcgatgaacc aggccttact gttgagctat actgtgggaa gtctgcttgg cccgtcattt
    67141 accgctatgc taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta
    67201 tcgtttatct atttgctgat gctgctgcgc aacgccggtc atacgccgaa acccgttgct
    67261 cacgtgtaaa tgaattcaag cagagtgtga acttactgtt tcacactctg cttttttgtt
    67321 tcttctatct gacttgcttt attccaaatt ttattcgttt aaaaataaaa tgtgcagcag
    67381 gttataattt tgcatttcgc tatttccgca cttcttattt gccgcgcata atccctcgtt
    67441 ttacagatgc ccctttaatt ttggcgaagg atttgtctat ggctgggaat gttcaggaaa
    67501 aacagctgcg atggtacaac attgcgctga tgtcttttat cactgtctgg ggttttggca
    67561 acgttgttaa taactatgcc aaccaggggc tggtggttgt tttttcatgg gtgtttatct
    67621 ttgcgctcta tttcacacct tatgcgctaa ttgttggtca gttaggctcg accttcaaag
    67681 atgggaaggg cggggtcagt acctggatta aacacacgat gggacccgga ctggcttatc
    67741 tcgccgcgtg gacctactgg gtggtgcata ttccctatct ggcacaaaaa ccccaggcaa
    67801 ttctgattgc gctcggttgg gcgatgaaag gcgacggttc gctaatcaaa gaatattcag
    67861 tcgtagcgtt acaggggtta acgctggtgc tgtttatctt ctttatgtgg gttgcttcac
    67921 gcggtatgaa atcgctgaaa atcgtcggtt ctgtggcagg gattgcgatg tttgttatgt
    67981 cgctcctgta tgtggcgatg gcggtaaccg cgcctgcgat tactgaagtg catattgcga
    68041 ccacaaacat tacctgggaa acgttcattc ctcatatcga ctttacctac attaccacta
    68101 tttcaatgct ggttttcgcg gttggcggag cagagaagat ttctccttac gttaatcaaa
    68161 cgcgcaaccc aggaaaagaa tttccaaaag ggatgctatg cctggcggtg atggttgcgg
    68221 tttgtgccat tctgggctca ctggcgatgg ggatgatgtt tgactcgcgt aatatcccgg
    68281 atgacttaat gactaacggt caatattacg cctttcagaa gctgggcgag tattacaaca
    68341 tgggtaatac tttaatggtg atttacgcca ttgcgaatac cctgggacaa gtagctgcgc
    68401 tggtattctc gattgatgcc ccgcttaaag tgctattagg tgatgctgac agcaaatata
    68461 ttccagccag tttatgtcgt accaacgctt ctggtacgcc cgttaatggc tattttctga
    68521 ccctgatgct ggtggcaatt ctgattatgc tgccgactct cggcatcggt gatatgaaca
    68581 atctctataa atggctgttg aatcttaact cggtagtgat gccgctgcgt tatttatggg
    68641 tatttgttgc atttattgca gtcgttcgcc tggcgcagaa atataaacca gaatatgtct
    68701 ttattcgtaa taagcctctg gcaatgaccg tcgggatttg gtgttttgcc tttaccgcct
    68761 ttgcctgttt gacggggatc ttcccgaaaa tggaagcctt cactgcagag tggaccttcc
    68821 agttggcgct gaatgttgca acgccgtttg tgctggtagg attaggactg attttcccgc
    68881 tgctggcgcg taaagcgaat agtaaataat tattgtggtc ggtgacgctg actctggcag
    68941 atctgacccg tttttacata atcgagaaat gcgcgcaagc cgcaggacat atactggcgg
    69001 tttggataat agatctggaa gcctggacgc tctgtgctcc agtctcccag tacacattct
    69061 aaacgaccag tatcaaggta ctccttgatc tgttcataga gcagataccc gataccagca
    69121 cccatcaaaa cggcctccag ttcagcatca acatcatcca gaatgatatt tcctgcgaca
    69181 gcaatttcca gctcctttgc aaattgccag tggaaaggtt tgccgctggg ataacgaaaa
    69241 acaacacatt gatgattaag cagatcatga ggatggcggg gtttgccata tcttgcaaag
    69301 tactctggcg ttgctgctac acataatttc actggcgggc cgatcgccac gctgatcata
    69361 tctttttcga cgatgccact aagtcgaacg ccagcgtcaa agccctgttg gacgatatcg
    69421 acaagagagt cgtcagttgt gagctcgact ttaatatcag gatattcacg tgtgaaacca
    69481 accagtaaag gcattaaaaa tatacgtgca gctacccggg cggcattgat tttgagcgtt
    69541 cctgtcggtg tcagacgaaa atcattcatc tcatctaaca tgatctgaat ttcgtcgaaa
    69601 gctgggcgca atcgttcata gagattagaa cccgcttccg ttaacgatac gcttcgggtt
    69661 gtccgattga ataagcgaat tttaagacgt tgttccagtg ttttaatact atggctaatg
    69721 gcggacgagg ataagcctaa ttcatcgccc gctgcacgaa agctttgatt acgagccacg
    69781 gcaaagaaag tggcaaagtc agacatattc atccgcatta gtgaaaatcc ttcattaact
    69841 catcctgaat catcacgttt acaggattat gccgcctgcg taaagtgcgc tccagaactt
    69901 aacgtggagg taaaattatg cagtctgaac gtatttattt ggtatgggcc catcctcgtc
    69961 atgattcatt gaccgcacat attgctgatg cgatccatca gcgggcaatg gagcggaaaa
    70021 tacaggtgac ggaactcgat ttatatcggc gtaatttcaa cccagtgatg acgctggaag
    70081 atgaaccaga ctggaagaat atggataaac gttattctcc agaggttcat cagctttatt
    70141 cagagctgct tgaacatgac acgttagtgg tggtttttcc tctctggtgg tacagcttcc
    70201 cggcaatgct aaaaggatat attgacagag tatggaataa tgggctggct tatggagatg
    70261 ggcagaaatt accattcaat aaagttcgtt gggtggcgct ggttggagga gacaaagaat
    70321 catttgtcca gatgggctgg gaaaaaaata taagcgatta tttgataaat atgtgcagtt
    70381 atcttggtat tgaagatgcc gatgtcactt tcttgtgtaa tacagtggta ttcgatgggc
    70441 aagaacttca tgcgagctat tatcagtcgt tattatctca ggtacgggat atggtagatg
    70501 cactataaaa tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa aaagcggcaa
    70561 ctcaataaag ttgccgcttt acggggaaat tagaacatta ccttatgacc gtactgctca
    70621 agaatgcctt tcacgcgttc catggtctct ttcttaggtg gtttaacacc gtcgagtttg
    70681 tactcttcac ccattgccac ccatttgtgt ttgcccagct cgtggtaggg gaggagttcg
    70741 attttctcaa cgttgcccat atcacgggta aattcaccga ggcgatgcgc tgaatcgtca
    70801 tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt tttgttcgcc
    70861 agatatttgg cgaactccag cgtgcggtgg ttggaaactc caaccagatt ttggtggatc
    70921 tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag cagttcatca
    70981 atcaccggat cgtaacgacg aacaaaaccg ttggtgtcca gacaggtatg aatgccttct
    71041 tttttgcagg cgcggaacca gtcacgaaca aactcagctt gtaggattgc ctcaccgccg
    71101 gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac cacctctttc
    71161 attaaatctt caacggtaac ttctttaccg ccatgcgtat cccaggtgtc gcggttatga
    71221 caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat acccgggccg
    71281 tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat tgcggtgttt
    71341 ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct
    71401 gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgc gctaaaaaag
    71461 gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta
    71521 attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg
    71581 agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat ttttccgggt
    71641 tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg tgctgaccac
    71701 cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg ttggtcttac
    71761 gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag gagataccat
    71821 ctttagcgta agcaaacggc agtttagcaa cggaagtcag agaggctact gcacctttct
    71881 ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt
    71941 ctggggtgtt acccgttttc ttaccataca caacgttaga agtgatggtc agaacagact
    72001 gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg aaacgttcta
    72061 ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc gggtattcgc
    72121 cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta actttcgcat
    72181 atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca cacgccatgg
    72241 tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt
    72301 gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag tccatgaagt
    72361 gatccatgcg ctccatcact tcatcatagt tcaggacatc gcctttgatc ggttcagact
    72421 tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg tacagcatgg
    72481 ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg atcatcgggc
    72541 ttacgcagca agcaatagcg tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt
    72601 tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc ttgaagttca
    72661 gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacgga cccatggtgt
    72721 acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgaccg tcgaggccca
    72781 taccaccgat agattcggtt gcccagatcg ggtcgccaga gaacagttca tcgtattccg
    72841 gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc atttcctgcg
    72901 cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca tccaggaagg
    72961 tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca gccaggtagc
    73021 cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag atgtcgtagc
    73081 cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca gcgatttctt
    73141 cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc tgcagagaag
    73201 tgaactgtgc cagtttgtct ttcatcaggt agtcgatacc gtacagcgca acgcgacggt
    73261 agtcaccgat gatacggcca cggccatatg catctggcag accggtcaga acaccagatt
    73321 tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg tgagttttac
    73381 ggtattcagt gaagattttt ttgatcatcg gatccagttc gcggttgtac gctttgcagg
    73441 aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc ggagcttcag
    73501 tctgcagacc aacgattttc tcaagctgct tgttgatgta gccagcgtcg tgagaggtga
    73561 tggtggaagc aacagcggtg tcaaagtcaa ctggcgcgtg agtgcggttt tccagtttaa
    73621 cgccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca gccaggaagg
    73681 actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg tttacttcat
    73741 tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca ttaagctcgg
    73801 acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc gacaaccatt
    73861 aatggtggtc gttttcacgc aggtaaatga cccagtatgt caacccaacc aacaaaccac
    73921 caccgataat gttgccgatc gtaaccggaa tcaggttatc agtgatgaaa ttcatcacgg
    73981 tcaggtgaga aaaattttcc ggtgcagaac cgactgcggt ccaaaattcc ggggatgcga
    74041 agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg tgctcaaaac
    74101 cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc atcaggctgc
    74161 ggccagaata actcatccat actgccagac ataccatcag gtttgccagg ataccaagac
    74221 agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg acgtttagtc
    74281 cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt acaaacagca
    74341 gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac tgaccccagg
    74401 tgatgcgccc actcgcctta gcaacaacaa ttaacacagt ggaagtaaag agatcggctc
    74461 cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc agttttgcca
    74521 tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg aatgcgattg
    74581 agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt ttcgttgctt
    74641 tatagacacc cgcttcttcg gccactttgg ccattgcagc aggaagtaaa agatcaaaag
    74701 ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat actaacaaag
    74761 cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc ccgtaactcg
    74821 catggttttt atgcaaatac ggagtaaata tttgattatc aaaataaaaa taaattttaa
    74881 aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta attaaaaggg
    74941 agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa ttaccgatat
    75001 ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg ggcattaaaa
    75061 aaacgccccg taatataact cagactaatc attaagccta cattgcgtag gctatttgat
    75121 tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacagcg
    75181 actgatgtgc agcaaacgcg tgcagatcgc tatctactgg ttgtaagcca taaaacgccg
    75241 cttcgccgct cattgccgca ctggcggctt ccacggcatc tgcgccgtac atgcgaacaa
    75301 acgcattcag atattgcagc ggctggcgat cttcttcctg cgccagtaat aacaacgttt
    75361 gcaggcagcg ataatagttg gcgcgttccg ggctgaatac cgatgagtta aactccatcg
    75421 tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt
    75481 ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc agacccaaca
    75541 gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg atgaggttca
    75601 ggtaatcttc tttttcccac tcgctgcttg gtagcgaaag aatcgtttca cgtaaatggc
    75661 tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg gacatgccag
    75721 gcacgataat gcggcaagcg tagacaccca gatgctcgta atcggcaata taaacttctt
    75781 tatcttcctt tttgaagata gccatcagcg tggcgaactc ttcttccgtg gtgccggaga
    75841 agctccagtc aacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac
    75901 cgctggaatc aataaagtgc gtttcaaggt tggtgtgctc ggccacttct tcatcatcga
    75961 aggttggcgg agtaaacaca tccagatctt tcaggccacg accttgcagc agctcggtca
    76021 cggtacgttc cagtgctaca ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc
    76081 cgttagcagg attaaacagt accacgcaga tcaccggata ttgaccgcca agcgaaccat
    76141 catatgcgaa gatcgggaaa ccttctgctt ccagtgtttc gatcgcttca accaccgctg
    76201 ggtaacgcgc cagcacgtct gccgggatct ctggcaggct gatgctttca gcaataatgc
    76261 ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttgcggg
    76321 tattacctgc ggacataccg ttggagacgt acaggttacc aatgatattc atcggaatat
    76381 aaacggtctg attgtcggac tgacgcgtaa acggcaggcc gcaaatacca cgatcttcgt
    76441 taccggattg caggtcaatc agcatgctac cggtcagttc attttccgga tcgtaaaatg
    76501 cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcggtc agtgggaacc
    76561 atttttcgtt gggataatgc acgaacggac cgttggcgat ggtttcgccc agccagaagt
    76621 ccgcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca gaagccagcg
    76681 ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa
    76741 tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca atctgaaagc
    76801 cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg gcatctttgc
    76861 cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata
    76921 cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt gactatgctt
    76981 ttagcggata aaccacgtta agagcataaa atgaaggcgt tcgatctcca ccgtatggca
    77041 tttgataaag tgccttttga tttccttggc gaagttgcac tacgtagtct ttataccttt
    77101 gtactggtct ttttgttcct caaaatgacc ggaagacgcg gtgtgcggca gatgtcgctg
    77161 tttgaagttt taatcattct gacgctggga tcagcggcgg gagatgtggc gttttatgat
    77221 gatgtgccga tggtcccggt acttatcgtc tttattactc tggcgttgtt ataccgcctg
    77281 gtaatgtggt tgatggcgca cagtgaaaaa ctggaagatc ttctggaagg caagccagtt
    77341 gttattattg aagatggcga gctggcctgg tcgaaactca ataactccaa catgacggaa
    77401 tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca ggtacgtctg
    77461 gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga caaggtgaaa
    77521 ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt gccggagtcg
    77581 gcggactatg cctgtattcg ttgtagtgaa atcattcata tgaaagcggg ggaaaaacaa
    77641 ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa acgggtgacc
    77701 tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt gtgatgcaag
    77761 ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa tgataaaacc
    77821 gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg ggttaatagc
    77881 aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg tccggcaatg
    77941 ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa cggtcttggt
    78001 acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc agaggaagcc
    78061 gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt attctgccat
    78121 ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga taaaaccacc
    78181 gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc gaaaaaatac
    78241 tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc ggttaagcca
    78301 atgcgtgaat ggcaactctc tgataatgcg gcttatatgc attactgccc gaatgaaacc
    78361 atcgacggta tcgccatcga cgagacgcca gacttcggca aagatgtggt ggtcgccgcc
    78421 gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg tgtaatttac
    78481 gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt tcgtgaagat
    78541 ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat cctcaacgat
    78601 aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg tctggtcttt
    78661 aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca gcaaaaagca
    78721 gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt ggcgaaagct
    78781 aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga caaattgttc
    78841 cttgaagagt cttttgctgc tggccttcat gcactgaaag gtcaccgtgt ggtcggcgga
    78901 atgcgcgcgt ctatttataa cgccatgccg ctggaaggcg ttaaagcgct gacagacttc
    78961 atggttgagt tcgaacgccg tcacggctaa tgccgaaatt ttgcttaatc cccacagcca
    79021 gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc tgacgttaca
    79081 acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg tttctaaccg
    79141 cgctttattg ctggcggcat tagcacacgg caaaacagta ttaaccaatc tgctggatag
    79201 cgatgacgtg cgccatatgc tgaatgcatt aacagcgtta ggggtaagct atacgctttc
    79261 agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg cagaaggtgc
    79321 cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg cagctctttg
    79381 tctgggtagc aatgatattg tgctgaccgg tgagccgcgt atgaaagaac gcccgattgg
    79441 tcatctggtg gatgctctgc gcctgggcgg ggcgaagatc acttacctgg aacaagaaaa
    79501 ttatccgccg ttgcgtttac agggcggctt taccggcggc aacgttgacg ttgatggctc
    79561 cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc cggaagatac
    79621 ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca cactcaatct
    79681 gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg tcgtaaaagg
    79741 cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat cttcggcttc
    79801 ttactttctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg gtattggacg
    79861 taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg gcgcgaccat
    79921 ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta ttgatatgga
    79981 tatgaaccat attcccgatg cggcgatgac cattgccacg gcggcgttat ttgcaaaagg
    80041 caccaccacg ctgcgcaata tctataactg gcgtgttaaa gaaaccgatc gcctgtttgc
    80101 gatggcaaca gaactgcgta aagtcggtgc ggaagtagaa gaggggcacg attacattcg
    80161 tatcactcca ccggaaaaac tgaactttgc cgagatcgcg acatacaatg atcaccggat
    80221 ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc ttgatcccaa
    80281 atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta gccaggcagc
    80341 ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt cggcacattg
    80401 tcggcgtttt ttttcggacc ttgtgagtca ttttgattaa tggtagcgtc gcttgtc
//
LOCUS       DAOFQD010000004        79971 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.004, whole genome shotgun sequence.
ACCESSION   DAOFQD010000004 DAOFQD010000000
VERSION     DAOFQD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.004"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..461
                     /locus_tag="QBF46_000242"
     CDS             <1..461
                     /locus_tag="QBF46_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDQ6672483.1"
                     /translation="GCQSNADDHAADVYQTDQLNTKQETKTVNIISILPAKVAVDNSQ
                     NKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVGAAAGSMVNDKTLVEG
                     VSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPNTKCPEKS"
     gene            477..1016
                     /locus_tag="QBF46_000243"
     CDS             477..1016
                     /locus_tag="QBF46_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDQ6672484.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1314..3266)
                     /locus_tag="QBF46_000244"
     CDS             complement(1314..3266)
                     /locus_tag="QBF46_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDQ6672485.1"
                     /translation="MSKKELKPELLTVGKLFTDNYLIPIYQRNYAWRAEQIEQLISDI
                     QDSVVGGQDNYFLGNLVVIKRGREDEFEVIDGQQRLTTLYLLLTFLEQDGEGEKPSVG
                     HAGHLQYESRARATEALLRVAQEAAKEHVRPQGSTSNADAGIHEGYSIINQFFKQNEN
                     LNRSREKFSDFLLTKVTVVRASLPPNTDLNRYFEIMNTRGQQLKQVDIVKARLMSKLP
                     NQYERECFAWVWDSCADMDSYVQMSLTRGDTSLRNKVFGDEWSWLEVTSFSSLMESRP
                     QSGINSSRQSSEGVSLSLDEALSKYAKELESNSTEDEGNERFRSTIEFPAFLLHVLRI
                     MKGDEVEDEGLLDDKRLIKSFDDAVNNVPDAKADWVRSFAFMLLKCRNLFDGFILKRQ
                     FTANIGDDGDWSLQRLKKGGGDKKPTPTYIHVFSASNGSLEEDGSADPHTRDVLLLQS
                     MLRITYTSPRTMHWITKVLRWLSVKAPRDVKHADLADLLKGYARSKVKETFSFEKDQQ
                     PQGFGISRIVFSYLDYLLLSDSSKRDFKFQFRTSIEHFYPQHPDKEQSGAVVSGSSLN
                     LLGNLALVSVSANSKFSNSLPRAKAENFKDTIELQSPKLKRMAEITRNTNWDDQQITA
                     HHEAMVTLLRDDVSLVEASGRSNPGR"
     gene            complement(3263..4543)
                     /locus_tag="QBF46_000245"
     CDS             complement(3263..4543)
                     /locus_tag="QBF46_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDQ6672486.1"
                     /translation="MAINGENGVSAVTVDELLKQGLRIPNYQRPYSWDVSTALQLVDD
                     ISEALRDTERKDIPYVLGAIILHDDGEYLNVVDGQQRLLTLRMILAALDPINHQISMS
                     GNSETPVSLVWIELQRRLSQLEDKKEFLDFICHKCQLVRIVTDDIDEAFRVFDSQNYR
                     GKPLAPHDLLKAHHLREMHDESAAMKVAVVEAWEAVNDEDLDRLFSTFLYRISKWSRG
                     ESSLEFTIRDIGMFKGISSRSHRSFSPNLRYHLAAQTAMPLLSAWSVSSTHDARNAGR
                     SRFQLDAPIIAGRSFFEMVTFMLDELKILEQEVIDRGFKNFGPSQSRYRYVYELFIAA
                     LLCYTNKFGDEDVDEVRNRLFAWAYALRVELLRVQFVSADNRARGKNDANKSPFVLLR
                     NAMTGSVVRKLPITSKPYSDNHEKELVAFIKGLQ"
     gene            complement(5053..5844)
                     /locus_tag="QBF46_000246"
     CDS             complement(5053..5844)
                     /locus_tag="QBF46_000246"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672487.1"
                     /translation="MALPPHAADWMNRAEIDYIGPFVKAWAAFNAWFRHASAQAQERA
                     MLDWIKVQPNPVRRGVLALLRNENGMAEAQALKMAISDLQIRLDDIHFEVTRKGVNEQ
                     ISLRSVCIAPKHFNRERIERNGHEYRAEKIAGGNIQITVTSVRNGNVKFQHVQVHYEP
                     NTIYGHADFTANLSKAQQTTLRQFYDGCNPRPMRDLLRGGAERLQVGAMEFQCSPEEL
                     LSGLIETIYAMRNALLHGEVDPDPRVLSCYEPAYRIVMLFLGCVR"
     gene            complement(5844..7235)
                     /locus_tag="QBF46_000247"
     CDS             complement(5844..7235)
                     /locus_tag="QBF46_000247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDQ6672488.1"
                     /translation="MEGLEATVVEYSDWRQDNDALRLDSEYQLKAHRAAIELVCTFGA
                     LQFKDDAPEIIHPHEIIREYVEEDGVWFLRAQNVRPMQIDPRNKVMISEADADKLLRN
                     VVKREDVLVTRTGANRGQCAFFDRDERAIASSHTFIIRPKTIDPEFLTLFLNTAHGVA
                     QIDKGVYGAAQPEVAPYYLGNIWVPKISPALMTRLKTAFSNSKAQAAKAEVGIGEAET
                     VLLAALGLANWTPPEPLAYTARASDVYVSGRFDARFFAPRIQALLDLLAVDGRTIADV
                     AAPRREKFRPDACASFDYIEISDIDGAGAAASTRLASEDAPSRATWHVRAGDIITSTV
                     RPIRRLSAQITPEQEGFVCSSGFVVVMPRDIAPEVLLTYLRLPVICELLDLFASASMY
                     PAITDADIFNLPLPHIADAVTDQVTQNVIAAKAAKARAARMLEAAKRAVEIAIENGEP
                     AAMAYLDQVEGAI"
     gene            complement(7244..8266)
                     /locus_tag="QBF46_000248"
     CDS             complement(7244..8266)
                     /locus_tag="QBF46_000248"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDQ6672489.1"
                     /translation="MMNPKEGETVIDTACGSAGFTVHSMFHVWRQIMRAMGREESHLF
                     TMEAKPPRCIDYVRNNVFAIDFDEKSVRVSRCLNLIAGDGETNVLHLNTLDWTKWDET
                     VKQDDWQDTYGDGWRRLRKLRENPRQTDYRSFGFDVLMANPPFAGDIKQSDMLSPYDL
                     AHKKNGKLERAVARDLLFIERNLDFLKPGGRMAIVLPQGRFNNSSDQRLREFIMERCR
                     ILGVVGLHPNTFKPHTGTKTSVLLVQKWNDDPAKGPLCPKVEDYDIFFATQQVESVDN
                     SGRKVYRKNPDGTFMRDTHGHFVVSHDLFNHDGLTEDGIAEAFEEFARKEGFSFFRAA
                     PSTKAV"
     gene            complement(8288..9268)
                     /locus_tag="QBF46_000249"
     CDS             complement(8288..9268)
                     /locus_tag="QBF46_000249"
                     /inference="COORDINATES: protein motif:HMM:NF024976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDQ6672490.1"
                     /translation="MKKQSSEFSEFVLFSEEEVRALDARVVLRDSRGKQVPYVACLVR
                     DKEVQLKPEERTRQLWLARLIDKLGYARSRIAIEYPITFGRDSSKRADIVVFDSDRPT
                     VPYIIVEVKQAKYKDGKEQLRSYAHATGAPLAVWSDGILAEVWHRKNPNYFMPIHELP
                     RADQTIEQVVDQPWTIQTLIDLEEERVKEGKFARSLRDLIEDMEDEVLANAGVDVFEE
                     VFKLIFTKLYDEMNSHRLGNALRFRNQNTAAQLKAAIQNLFDDAKRKWPGVFLDDERI
                     RLSPDHLQVCIGSLEEWKLFNSNLDVIDDAFEYLVSKSSKGEKGQYFTPR"
     gene            complement(9405..9985)
                     /locus_tag="QBF46_000250"
                     /pseudo
     CDS             complement(9405..9985)
                     /locus_tag="QBF46_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017347760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(10273..10527)
                     /locus_tag="QBF46_000251"
     CDS             complement(10273..10527)
                     /locus_tag="QBF46_000251"
                     /inference="COORDINATES: protein motif:HMM:NF024115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system ptaRNA1 family
                     toxin"
                     /protein_id="HDQ6672491.1"
                     /translation="MKDRSHDTAMAECFRADPLYAVELLTEVRREGNPAELAVLLRQM
                     ATASVDAVGLDDAETGLATREDFQEAMRALRQACANDVLR"
     gene            complement(10792..10941)
                     /locus_tag="QBF46_000252"
     CDS             complement(10792..10941)
                     /locus_tag="QBF46_000252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672492.1"
                     /translation="MEVKFSRYDTADYLKTEDDIAAYLDAVTEDGDPLTDRGGVWRLG
                     PPPQP"
     gene            11713..11922
                     /locus_tag="QBF46_000253"
     CDS             11713..11922
                     /locus_tag="QBF46_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672493.1"
                     /translation="MARKTLAERRSDALSELEMAKARLAKLDNEAAERIGRIAIKSGL
                     VNLELTDDQVREEFDRIVERISKGN"
     gene            11925..12149
                     /locus_tag="QBF46_000254"
     CDS             11925..12149
                     /locus_tag="QBF46_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672494.1"
                     /translation="MKTAHRISALANQLNELQACLGRASGRPSNSVMEAQRIAAELAS
                     SLEDWHLETLHIPEPERDLYRAQNPYYAAH"
     gene            12418..13209
                     /gene="trbJ"
                     /locus_tag="QBF46_000255"
     CDS             12418..13209
                     /gene="trbJ"
                     /locus_tag="QBF46_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDQ6672495.1"
                     /translation="MELESQKMSAYAARKFGRGAALALAMASAGLGVSLYMAPAPAYA
                     IYCSNCSTFYQQMYEYAEAVNTQLNTAQQLQTQIQQYDNMIKQGMSLPSRMFNTITGD
                     LQRVASVYNSAQSLGRNISNLDEQFRQQFKGYDSYLNSIGQGQNNMPQRYRDWAQSGF
                     DNARTAMQAAGVNTSTFEDENAMLDQLVSRSQSAVGRQQAIQAGNEIAAQNVQQLQKL
                     RDLVATQITLQGNYMAKQTEQEAVKDAASERFWGGTDTPGPSKGF"
     gene            13212..13394
                     /locus_tag="QBF46_000256"
     CDS             13212..13394
                     /locus_tag="QBF46_000256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672496.1"
                     /translation="MTISKILLLVLCLGSAVGGAVMTSVVISSGEECSASQKQKAAAT
                     KKFWEGKDEPGPSKGF"
     gene            13436..14935
                     /gene="trbL"
                     /locus_tag="QBF46_000257"
     CDS             13436..14935
                     /gene="trbL"
                     /locus_tag="QBF46_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HDQ6672497.1"
                     /translation="MLLMTGSAMAAGLQSNVEMNGLLTDIHNASAQWGPKLQGYARNL
                     LFLLAGIQFVWTFGNLIWQSADVGEMFRELIKFVMTIGFFLALLTYSTEWSTAIVNSF
                     REAGSQASGFAQELMPGDVFTLAIKLAQKVLSNISLFDLETAVVIALSGVIILLCFAF
                     IAAFMFVTLVESYVIINAAVLFIGFGGSQWTREFAIAPLRYAVAVGAKLFVLTLIVGL
                     IMSVSNRWFANYDGTIGPTLTLLGLSLVCAYLSRSIPELIAGMITGSSFGGGSAIGGM
                     AMAAAAAATGLAAGTMMAGGANAAGGAAGALGGGSGGGAGSGLADSINASMAGGQQNP
                     AASAMPSMNSAATTGTATGGSAAAQSAGSRVGGSQAGKSPSGSGINSQSDTPRSSGLS
                     GPQQAAQTLKKAASGANNLASVAARGLGTLAAISVPGMEGAASMGGGPPPQMPDSDEG
                     DEKRPANSGVSADVAEETNVIRPAQEPSAAPAQPNGVEKSNNPKQEGEA"
     gene            15102..15410
                     /locus_tag="QBF46_000258"
     CDS             15102..15410
                     /locus_tag="QBF46_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672498.1"
                     /translation="MIEYLIFCVVAFAAAFGMYLLREYLRSRGYGPQLDAFARWLEPR
                     EQRMNRYRFKAANGLFGAAQSINRLPLFGSKHQVVMIEQLRMAIMSEQALRERQQRRK
                     "
     gene            complement(16152..16388)
                     /locus_tag="QBF46_000259"
     CDS             complement(16152..16388)
                     /locus_tag="QBF46_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672499.1"
                     /translation="MGAKSRHDPKAWPLRGSHRFAAAPLPHERRSLRPNALAELAAVG
                     WRLDEYAKGKWETGDPRHPTVTLLPLFNGTSPPV"
     gene            complement(16718..17776)
                     /locus_tag="QBF46_000260"
     CDS             complement(16718..17776)
                     /locus_tag="QBF46_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDQ6672500.1"
                     /translation="MSSRNSGLKPIGAVIGDSGGLLERLENIRKRNSDRTPAAERGRP
                     SNVSTVHEQMERAKERDKALSLVRKPPQGDAQPDFFAPSLYDVGGRDNRSVMDVAVFR
                     LSKKDKRAGELIRYDLPDGYVEVSAGAHGMASVWDYDIVLMMVSHLTEAMNRYREGKG
                     GKPGRVFRLHVSDILRFARRGNGSSQVKGVEAALDRLRGTTIKTVRESGKFRTTEAEG
                     LIARYRVLSRTDTKKISSVEIEAPEWIYREVTEGKRPEVLTVHRDYFLIEPGIGRFIY
                     RLARRAAGRDSAKWAFKTLYERSGSGGSFKKFCFFLRGIIKADDLPEYALCEEAGKDG
                     PLLVMRHRNSSSEPLGGS"
     gene            complement(17857..18087)
                     /locus_tag="QBF46_000261"
     CDS             complement(17857..18087)
                     /locus_tag="QBF46_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006073477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDQ6672501.1"
                     /translation="MERELRRVSKVLISKKQLLEMIPLCERTIYNFEKQGKFPKRIAL
                     SSRKVVWDLAEVEAWIDACKHSGSVPRPGMGA"
     gene            complement(18371..19579)
                     /locus_tag="QBF46_000262"
     CDS             complement(18371..19579)
                     /locus_tag="QBF46_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6672502.1"
                     /translation="MLTDTKLRNLKPKDKLYKVNDRDGLYVAITPAGSISFRYNYSIH
                     GRQETITFGRYGVGGITLAEARERLGEAKKMIAAGKSPAKEKARDKARVKDAENFGAW
                     GEKWLRGYQMADVTRDMRRATFERELKPKFGNQKLTEITHEDLRALTDAIVERGAPAT
                     AVLCREIVMQVFRWAIERGQKVENPADLVRPSTIAKFEPRDRALTPDEIGLMYQYMER
                     IGTTPSIRAAAKLLLLTMVRKSELTNATWSEINFTEALWTIPKERMKRRNPHLVFLSR
                     QALDIFIALKTFAGGSEYVLPSRYDSDLPMSSATLNQVLTLTYRLAQKEGQSLGKFGP
                     HDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYRDQRRAMLQDWADMIDE
                     WTIRKPRLKD"
     gene            complement(19888..21465)
                     /gene="guaA"
                     /locus_tag="QBF46_000263"
     CDS             complement(19888..21465)
                     /gene="guaA"
                     /locus_tag="QBF46_000263"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDQ6672503.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(21534..23000)
                     /gene="guaB"
                     /locus_tag="QBF46_000264"
     CDS             complement(21534..23000)
                     /gene="guaB"
                     /locus_tag="QBF46_000264"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDQ6672504.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            23162..24532
                     /gene="xseA"
                     /locus_tag="QBF46_000265"
     CDS             23162..24532
                     /gene="xseA"
                     /locus_tag="QBF46_000265"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDQ6672505.1"
                     /translation="MLPSQFPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFMQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(24529..24744)
                     /locus_tag="QBF46_000266"
     CDS             complement(24529..24744)
                     /locus_tag="QBF46_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDQ6672506.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(24814..26286)
                     /gene="der"
                     /locus_tag="QBF46_000267"
     CDS             complement(24814..26286)
                     /gene="der"
                     /locus_tag="QBF46_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDQ6672507.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(26404..27582)
                     /gene="bamB"
                     /locus_tag="QBF46_000268"
     CDS             complement(26404..27582)
                     /gene="bamB"
                     /locus_tag="QBF46_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDQ6672508.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(27593..28213)
                     /gene="yfgM"
                     /locus_tag="QBF46_000269"
     CDS             complement(27593..28213)
                     /gene="yfgM"
                     /locus_tag="QBF46_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDQ6672509.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAITAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDSIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(28231..29505)
                     /gene="hisS"
                     /locus_tag="QBF46_000270"
     CDS             complement(28231..29505)
                     /gene="hisS"
                     /locus_tag="QBF46_000270"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDQ6672510.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(29616..30734)
                     /gene="ispG"
                     /locus_tag="QBF46_000271"
                     /gene_synonym="gcpE"
     CDS             complement(29616..30734)
                     /gene="ispG"
                     /locus_tag="QBF46_000271"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDQ6672511.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AIRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(30761..31774)
                     /gene="rodZ"
                     /locus_tag="QBF46_000272"
     CDS             complement(30761..31774)
                     /gene="rodZ"
                     /locus_tag="QBF46_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDQ6672512.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(32059..33213)
                     /gene="trmG/rlmN"
                     /locus_tag="QBF46_000273"
     CDS             complement(32059..33213)
                     /gene="trmG/rlmN"
                     /locus_tag="QBF46_000273"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDQ6672513.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(33363..33794)
                     /gene="ndk"
                     /locus_tag="QBF46_000274"
     CDS             complement(33363..33794)
                     /gene="ndk"
                     /locus_tag="QBF46_000274"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDQ6672514.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(33943..36255)
                     /gene="pbpC"
                     /locus_tag="QBF46_000275"
     CDS             complement(33943..36255)
                     /gene="pbpC"
                     /locus_tag="QBF46_000275"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDQ6672515.1"
                     /translation="MPRLSTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVKFVMQ"
     gene            complement(36256..41217)
                     /gene="yfhM"
                     /locus_tag="QBF46_000276"
     CDS             complement(36256..41217)
                     /gene="yfhM"
                     /locus_tag="QBF46_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDQ6672516.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHLTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFEIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHINIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEQLTGEKAQNSNLNSDELATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            41424..42269
                     /gene="sseA"
                     /locus_tag="QBF46_000277"
     CDS             41424..42269
                     /gene="sseA"
                     /locus_tag="QBF46_000277"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDQ6672517.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQVIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(42763..43539)
                     /gene="sseB"
                     /locus_tag="QBF46_000278"
     CDS             complement(42763..43539)
                     /gene="sseB"
                     /locus_tag="QBF46_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDQ6672518.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLGSTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(43681..44964)
                     /gene="pepB"
                     /locus_tag="QBF46_000279"
     CDS             complement(43681..44964)
                     /gene="pepB"
                     /locus_tag="QBF46_000279"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDQ6672519.1"
                     /translation="MTKAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVVWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALADRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(45142..45342)
                     /gene="iscX"
                     /locus_tag="QBF46_000280"
     CDS             complement(45142..45342)
                     /gene="iscX"
                     /locus_tag="QBF46_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDQ6672520.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(45354..45689)
                     /gene="fdx"
                     /locus_tag="QBF46_000281"
     CDS             complement(45354..45689)
                     /gene="fdx"
                     /locus_tag="QBF46_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDQ6672521.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(45691..47541)
                     /gene="hscA"
                     /locus_tag="QBF46_000282"
     CDS             complement(45691..47541)
                     /gene="hscA"
                     /locus_tag="QBF46_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDQ6672522.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(47558..48073)
                     /gene="hscB"
                     /locus_tag="QBF46_000283"
     CDS             complement(47558..48073)
                     /gene="hscB"
                     /locus_tag="QBF46_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDQ6672523.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(48169..48492)
                     /gene="iscA"
                     /locus_tag="QBF46_000284"
     CDS             complement(48169..48492)
                     /gene="iscA"
                     /locus_tag="QBF46_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDQ6672524.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(48509..48895)
                     /gene="iscU"
                     /locus_tag="QBF46_000285"
     CDS             complement(48509..48895)
                     /gene="iscU"
                     /locus_tag="QBF46_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDQ6672525.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(48923..50137)
                     /gene="iscS"
                     /locus_tag="QBF46_000286"
     CDS             complement(48923..50137)
                     /gene="iscS"
                     /locus_tag="QBF46_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDQ6672526.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(50249..50737)
                     /gene="iscR"
                     /locus_tag="QBF46_000287"
     CDS             complement(50249..50737)
                     /gene="iscR"
                     /locus_tag="QBF46_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDQ6672527.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(51007..51747)
                     /gene="trmJ"
                     /locus_tag="QBF46_000288"
     CDS             complement(51007..51747)
                     /gene="trmJ"
                     /locus_tag="QBF46_000288"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDQ6672528.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            51866..52669
                     /gene="suhB"
                     /locus_tag="QBF46_000289"
     CDS             51866..52669
                     /gene="suhB"
                     /locus_tag="QBF46_000289"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDQ6672529.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            52814..53668
                     /locus_tag="QBF46_000290"
     CDS             52814..53668
                     /locus_tag="QBF46_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ6672530.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKLYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYHGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKMPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            53859..55139
                     /gene="csiE"
                     /locus_tag="QBF46_000291"
     CDS             53859..55139
                     /gene="csiE"
                     /locus_tag="QBF46_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDQ6672531.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFLPGLTATTATFSELNGVAD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(55131..56270)
                     /locus_tag="QBF46_000292"
     CDS             complement(55131..56270)
                     /locus_tag="QBF46_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDQ6672532.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(56430..57320)
                     /gene="hcaR"
                     /locus_tag="QBF46_000293"
     CDS             complement(56430..57320)
                     /gene="hcaR"
                     /locus_tag="QBF46_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDQ6672533.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            57456..58817
                     /locus_tag="QBF46_000294"
     CDS             57456..58817
                     /locus_tag="QBF46_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDQ6672534.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            58814..59332
                     /locus_tag="QBF46_000295"
     CDS             58814..59332
                     /locus_tag="QBF46_000295"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDQ6672535.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            59332..59652
                     /locus_tag="QBF46_000296"
     CDS             59332..59652
                     /locus_tag="QBF46_000296"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDQ6672536.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            59649..60461
                     /gene="hcaB"
                     /locus_tag="QBF46_000297"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             59649..60461
                     /gene="hcaB"
                     /locus_tag="QBF46_000297"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDQ6672537.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            60471..61673
                     /locus_tag="QBF46_000298"
     CDS             60471..61673
                     /locus_tag="QBF46_000298"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDQ6672538.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFFDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDTLGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTVNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            61770..62192
                     /locus_tag="QBF46_000299"
     CDS             61770..62192
                     /locus_tag="QBF46_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDQ6672539.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(62240..63112)
                     /locus_tag="QBF46_000300"
     CDS             complement(62240..63112)
                     /locus_tag="QBF46_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDQ6672540.1"
                     /translation="MTIYTLSHGALKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLSQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLLALAPG
                     ESTTSEMSLRVALL"
     gene            complement(63124..64218)
                     /locus_tag="QBF46_000301"
     CDS             complement(63124..64218)
                     /locus_tag="QBF46_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDQ6672541.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFTLEQAGDAYALMASGKCGKVVINFSD"
     gene            complement(64251..65249)
                     /locus_tag="QBF46_000302"
     CDS             complement(64251..65249)
                     /locus_tag="QBF46_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6672542.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNS
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(65273..66784)
                     /locus_tag="QBF46_000303"
     CDS             complement(65273..66784)
                     /locus_tag="QBF46_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDQ6672543.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLSQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(66807..67790)
                     /locus_tag="QBF46_000304"
     CDS             complement(66807..67790)
                     /locus_tag="QBF46_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDQ6672544.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGNISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(67887..71168)
                     /locus_tag="QBF46_000305"
     CDS             complement(67887..71168)
                     /locus_tag="QBF46_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDQ6672545.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLQVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGEPVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGSTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQARELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            71286..72479
                     /locus_tag="QBF46_000306"
     CDS             71286..72479
                     /locus_tag="QBF46_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDQ6672546.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESHTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDHTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(72677..73930)
                     /gene="glyA"
                     /locus_tag="QBF46_000307"
     CDS             complement(72677..73930)
                     /gene="glyA"
                     /locus_tag="QBF46_000307"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDQ6672547.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            74258..75448
                     /gene="hmpA"
                     /locus_tag="QBF46_000308"
     CDS             74258..75448
                     /gene="hmpA"
                     /locus_tag="QBF46_000308"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDQ6672548.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(75493..75831)
                     /gene="glnB"
                     /locus_tag="QBF46_000309"
     CDS             complement(75493..75831)
                     /gene="glnB"
                     /locus_tag="QBF46_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDQ6672549.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(75892..77226)
                     /gene="glrR"
                     /locus_tag="QBF46_000310"
     CDS             complement(75892..77226)
                     /gene="glrR"
                     /locus_tag="QBF46_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDQ6672550.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAVIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSQLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(77216..77929)
                     /gene="qseG"
                     /locus_tag="QBF46_000311"
     CDS             complement(77216..77929)
                     /gene="qseG"
                     /locus_tag="QBF46_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDQ6672551.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(78094..79521)
                     /gene="qseE"
                     /locus_tag="QBF46_000312"
     CDS             complement(78094..79521)
                     /gene="qseE"
                     /locus_tag="QBF46_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDQ6672552.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
BASE COUNT        18750 a        22081 c        20353 g        18787 t
ORIGIN      
        1 caggatgcca gtcaaatgct gacgatcatg ccgccgatgt ttatcaaacc gatcaactga
       61 ataccaaaca agaaactaaa accgttaata ttatttccat tcttcccgca aaagttgccg
      121 tagacaactc ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt ggcgcagtcg
      181 ctggcggtgt tatcggccac aacgtcgggt ctggcagcaa ttccggaacg acggcaggtg
      241 cagttggcgg cggagctgta ggcgcggcag cgggttctat ggtgaatgat aaaaccttag
      301 tggaaggtgt ttctttaacg tataaggaag gcaccaaagt gtatacctct acccaggtgg
      361 gtaaagagtg ccagtttacg acaggtttag ccgttgttat taccacaacg tataacgaaa
      421 cgcgtattca gccaaatact aaatgtcctg aaaagagcta ataatcagga ggagtcatga
      481 agaaagtttt tctttgcgcc atcttggcct ccttaagcta tccggctatc gcctcatcat
      541 tgcaggatca actctctgct gtcgcagaag cggaacagca aggtaaaaat gaagagcaaa
      601 ggcagcatga cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc
      661 gcgcaaatgc ccaggctgcc gctaacaaaa gagcggcaac ggcagcggca aataagaaag
      721 ctcgtcagga taaactggac gccgaagcca ctgcggacaa aaaacgcgat caaagttatg
      781 aagatgagct acgcagctta gagattcaga aacaaaaact ggcgctggca aaagaagaag
      841 cccgcgttaa gcgagaaaac gaatttatcg atcaggaact gaagcacaaa gctgcgcaaa
      901 ccgatgtggt gcaatctgaa gctgacgcca acagaaatat gactgaaggc ggtcgcgatc
      961 tgatgaaaag cgtgggcaaa gcagaagaga acaaatcgga cagctggttt aattaatcga
     1021 tgttagtaac ttcaagccta taattcttga agataaaaaa ccctctgtag taacagaggg
     1081 ttttgttcat tcatagtgca gggtcaaatc attcccactc gatgatcaat gctgtcttaa
     1141 aacactataa aatcaataaa atagcattat atctgcatgt cgataccata caaaatacca
     1201 tggtaagaaa acttggagac tgtactgatc gtgtcgcccg acgcaaaacg cttttagact
     1261 gacaacttgc acaccacaaa ggatattcgt ccaaaagcgg ttattcaaag aagtcaacgc
     1321 cctgggttgg atcttccgct tgcctccacc agacttacat catcacgaag caatgtcacc
     1381 atcgcttcat gatgtgctgt tatctgctga tcgtcccagt ttgtatttcg ggtgatttcc
     1441 gccattctct tgagcttggg actttgaagt tcaattgtat ccttgaagtt ttcggccttt
     1501 gctctgggga ggctattgct gaattttgag ttcgcgctga cgcttacgag cgcgaggttg
     1561 ccaagcaggt taaggctaga acctgaaaca accgcgcccg actgttcttt gtcaggatgc
     1621 tgtggataga agtgctcaat ggaagttctg aactgaaatt tgaaatctcg ttttgaggag
     1681 tcactcaaca gaagatagtc gagatacgaa aagacgatac ggctgatacc aaacccttgt
     1741 ggttgctgat ctttctcaaa ggagaatgtc tctttaacct tgctgcgggc atatcccttc
     1801 aacaaatcgg caagatccgc atgtttcacg tctcgagggg ctttaacgga gagccagcga
     1861 agaactttgg taatccagtg catcgttcgc ggagaagtgt aggtaatccg aagcatggac
     1921 tgaagcagca atacatctcg ggtatgtggg tcagcactcc cgtcctcctc taagcttcca
     1981 ttgctggccg agaaaacatg gatgtacgtc ggggttggct tcttgtcccc gcctcctttt
     2041 ttcagtcgct ggagagacca atcgccatcg tcaccgatgt ttgccgtgaa ttgacgcttc
     2101 aagatgaagc catcaaagag gttgcggcac ttgagaagca taaatgcaaa actacgcacc
     2161 caatcggctt tagcatcggg tacattattt acggcgtcgt caaatgactt gatgaggcgc
     2221 ttgtcgtcaa gcaggccttc gtcttcgacc tcatcgcctt tcataattct tagaacgtgt
     2281 agcagaaagg ccggaaactc aatagtcgaa cgaaaccgct cgttgccttc gtcttcagtg
     2341 gaatttgact cgagctcttt agcgtatttt gacaacgcct catccaagga caacgatacc
     2401 ccttcggagg attgccttga tgaattgatg ccggattgcg gacgactttc cattagcgaa
     2461 gagaagctgg tcacctccag ccaggaccat tcatcaccaa atactttgtt tcgcaaactt
     2521 gtgtcaccac gggtcagaga catctggaca taagaatcca tgtcagcaca ggaatcccaa
     2581 acccacgcaa agcattcgcg ttcatattga ttgggcagtt tgctcatcag gcgagcctta
     2641 acaatatcaa cctgcttgag ttgctgccca cgagtgttca tgatctcgaa gtagcgattg
     2701 aggtcggtat tgggtggcag cgaggcacgg acgacagtca ccttcgttag caagaaatcg
     2761 gagaattttt cgcgtgagcg attaaggttt tcattttgtt tgaagaactg attgatgatg
     2821 ctgtagcctt catgaatgcc agcatctgca ttactcgtgg agccctgcgg tcgaacgtgc
     2881 tccttagccg cctcctgtgc tactcgcagc agggcctcag ttgcccgtgc gcgcgactca
     2941 tattgcaagt ggcctgcatg gccaaccgac ggcttctcac cctcaccgtc ttgctccagg
     3001 aaggtaagaa gcagatagag ggttgtaagt cgttgttgtc catctatgac ctcgaactca
     3061 tcctctctac cccgcttgat tacaacaagg ttcccaagaa aatagttgtc ctggccccct
     3121 accaccgagt cttgaatatc gctaataagt tgctcaatct gttcggcccg ccaagcatag
     3181 ttccgttgat aaataggaat cagataattg tcagtgaata acttgcctac tgttaacagt
     3241 tcaggtttca actccttctt gctcattgca gccccttgat aaaagcaacg agttcctttt
     3301 cgtggttatc gctgtatggc ttacttgtta ttggaagttt gcggacaaca cttccagtca
     3361 tcgcattacg tagcaaaaca aaaggcgatt tatttgcatc gtttttacct cgcgctcggt
     3421 tgtcagcaga tacaaattgc acacgaagta actcgacccg caaggcatat gcccatgcga
     3481 ataacctatt tcgcacttca tctacatctt catcgccaaa cttgtttgta tagcaaagca
     3541 gcgcggcaat gaacaattcg tacacgtagc ggtagcgact ttgagatggg ccaaagtttt
     3601 tgaaacctct gtcaataacc tcttgctcca gtatcttcaa ttcgtcaagc atgaatgtca
     3661 ccatctcgaa aaatgagcgt cctgcgatta tcggggcatc aagctgaaag cggctgcgcc
     3721 cggcatttcg tgcatcatgt gtagaggata ccgaccatgc acttagaagt ggcatagcgg
     3781 tttgtgctgc aaggtgatat cgcagatttg gcgaaaatga cctgtgggat cgtgatgaaa
     3841 tgcccttgaa catgccgatg tcccgaatag taaattcgag cgagctttcg cctcgtgacc
     3901 acttggatat ccggtataaa aaagtagaaa aaagcctgtc cagatcctca tcgtttacgg
     3961 cttcccaagc ctcaacgaca gccactttca tagcagcgga ctcatcgtgc atttcgcgca
     4021 ggtggtgtgc tttgagcaag tcgtgagggg caagcggttt gccgcgatag ttctgggaat
     4081 caaagacacg aaatgcctca tcaatatcgt ccgtcacaat acgcaccaac tgacatttgt
     4141 gacagataaa atccaaaaac tcttttttat cttccaactg cgacaggcgc ctttgaagct
     4201 ctatccaaac tagggagaca ggagtttcgc tgttaccaga catcgaaatt tgatgattta
     4261 tcggatctaa ggccgcaaga atcattcgca aagtcagcag gcgctgctgc ccgtcgacta
     4321 cattgagata ctcaccatca tcgtgaagaa tgattgcacc caaaacatag ggaatatctt
     4381 ttcgttcggt atctcgaaga gcttcgctga tatcatccac cagttgtaga gcagtcgata
     4441 catcccaact gtatgggcgt tgatagtttg gtatccgaag gccttgcttg agtagttcat
     4501 ccacggtgac tgcactaaca ccgttctctc cgttaatcgc cattcctatg tcccttcatt
     4561 gacgcttttc tattttctca gcgtattttc cgcaaacttt attcacctat atggatactt
     4621 cgattccgag tggcggctgt cttgttcaca atttctatca cgaggcgctc ctctggcgca
     4681 tatttaacga tctggggcat gggtcgagag agcggttctg atcaaagcga gcatcgatgg
     4741 catcagcaca gcgctgcaat gcacccggtt gtcgatacca tggcatttgc tgatacggtc
     4801 acatggtatt tgctatatct tcgattcagc gtcggcctta aaccataaaa aatgccattg
     4861 ataatgcagt catatcatca atggcactca ctaaatcaaa taaaacaata ggctaaataa
     4921 tagcctcact cccactctat tatcaactgc ctatatatca tattaattta cttagattaa
     4981 atatcaaata aaatatcaat gccatgacta atgccatatc taaaaaatat gaacacaggg
     5041 tacatctcct gcttatctca cgcagcctag aaacagcatt acgatccgat aagctggctc
     5101 atagcaagac agtaccctcg ggtcggggtc tacttcgccg tgtagtaggg cgtttcgcat
     5161 ggcgtagatc gtttcaatca atcccgacaa tagctcttca ggggagcact gaaactccat
     5221 cgccccgact tgcaggcgtt cggctccacc acgcagtagg tcgcgcatcg ggcgcgggtt
     5281 gcagccgtca tagaactgcc gcagcgtcgt ttgctgggct ttagagaggt tcgcagtaaa
     5341 gtctgcatgg ccatagatcg tgttcggctc ataatgcacc tgaacgtgct ggaatttcac
     5401 gtttccattg cgaactgagg tgacggtgat ctggatgttt ccgcccgcga tcttctcagc
     5461 cctgtattca tgcccgttgc gttcgatccg ctcacggttg aaatgcttag gcgcgatgca
     5521 aacgctgcga agtgaaattt gctcatttac tcccttccgc gtcacttcga agtggatgtc
     5581 atccaatcgg atttgcagat cggagatcgc cattttaagc gcctgcgcct cggccatacc
     5641 attctcattg cgcagcaggg cgaggacacc tcggcgaact gggttcggct ggactttgat
     5701 ccagtcgagc attgcccgtt cttgcgcctg tgccgaggca tggcgaaacc acgcattgaa
     5761 cgcggcccag gctttgacga aggggccaat gtagtcgatt tcggcgcggt tcatccagtc
     5821 ggcggcgtgg ggaggcaggg ccatcagatt gccccctcta cttggtcaag gtaggccatc
     5881 gcagcgggct ccccgttctc aatcgcaatc tcgacggcgc gtttagccgc ctccaacatt
     5941 ctggccgcgc gtgccttagc ggccttcgcg gcaatcacgt tctgcgtcac ttgatcggtc
     6001 acggcgtccg cgatatgcgg cagcggcagg ttgaagatgt cggcgtcagt gatcgccgga
     6061 tacatgctgg cgctggcaaa caggtccagc agttcacaga tcactggcag gcgcagatag
     6121 gtgagcaaaa cctcgggagc gatgtcgcgg ggcatcacca caacgaaccc ggacgagcag
     6181 acgaaaccct cttgctcggg cgtgatctgg gccgacaggc ggcggatcgg gcgcacggtg
     6241 gaggtgatga tgtcccccgc ccgcacatgc catgtggcgc ggctgggcgc gtcttccgat
     6301 gcaaggcggg tgctggcagc cgccccggct ccgtcgatgt cgctgatctc gatatagtcg
     6361 aatgaagcgc aggcatcggg gcggaacttt tcgcgccgtg gggcggcaac gtcagcaatg
     6421 gtgcgcccgt ccacggccag caggtcaagc aaagcctgaa tacgcggggc aaaaaagcgg
     6481 gcatcgaacc ggcccgagac gtagacatcc gacgcgcggg cggtgtaagc aagcggttcg
     6541 ggcggggtcc agttggcaag accgagggcg gcaagcaaga ccgtttctgc ttcaccaatg
     6601 ccgacttctg ctttagcggc ttgcgccttt gagttcgaaa acgccgtctt tagccgtgtc
     6661 ataagggctg gactaatctt gggaacccag atgttcccaa ggtaataagg ggctacctca
     6721 ggctgcgcag ccccatagac ccctttgtca atctgcgcga caccgtgggc ggtgttcagg
     6781 aagagcgtta gaaactcagg gtcaatggtc ttggggcgaa tgatgaaagt gtgcgaggat
     6841 gctatcgccc gctcgtcacg atcaaagaac gcacattggc cgcgattggc gccggttcgg
     6901 gtgacaagaa cgtcctctcg tttcacaaca ttgcggagca gcttgtcggc atcagcctct
     6961 gaaatcatca ccttattacg tgggtcaatt tgcataggac ggacattctg cgcccgaagg
     7021 aaccagacgc cgtcctcttc gacatattca cgaatgatct catgcggatg aatgatctca
     7081 ggggcgtcgt ccttaaactg gagcgcgcca aaggtgcata ccagctcaat cgcagcgcga
     7141 tgggccttga gttggtattc gctgtccaac cgcagcgcgt cgttgtcctg tcgccaatcc
     7201 gaatattcaa ctaccgtggc ttcaagcccc tccaacaggc cgcttagacg gccttggtcg
     7261 aaggggcggc ccgaaaaaaa ctgaagccct ccttgcgggc gaattcctcg aaggcttccg
     7321 cgatcccgtc ctcagtcagg ccgtcgtgat tgaacagatc atgcgacacg acaaaatggc
     7381 cgtgcgtatc gcgcatgaac gtgccgtccg ggttctttcg atagaccttt cggcccgagt
     7441 tgtcgacgct ttccacctgc tgcgtggcaa agaagatgtc gtaatcctca accttcgggc
     7501 atagcggccc cttggccgga tcgtcattcc acttctgcac cagcaagaca ctggtcttgg
     7561 tgccggtgtg cggcttgaag gtgttcgggt gcagccccac cacgccgagg atgcggcaac
     7621 gttccatgat gaactcgcgc aggcgctgat ccgacgagtt gttgaaccgc ccctgcggca
     7681 ggacaatcgc cattcgccca ccgggcttga ggaagtccag attgcgttcg atgaacagca
     7741 ggtcgcgggc caccgcgcgt tcgagcttgc cattcttctt gtgggcaagg tcgtagggcg
     7801 acagcatgtc agactgcttg atatcccccg cgaagggcgg gttcgccatc agtacgtcaa
     7861 agccgaagct gcggtaatcg gtctggcgcg ggttctcgcg cagcttgcga agccgccgcc
     7921 aaccgtcgcc ataggtgtcc tgccagtcgt cctgcttgac cgtctcgtcc cacttcgtcc
     7981 agtcgagcgt gttcaagtgc aggacgttgg tttcaccatc ccccgcgatc aggttcaggc
     8041 agcgcgagac tcggacgctc ttctcgtcaa agtcgatggc gaacacgtta ttgcgcacgt
     8101 agtcgatgca ccgcggcggt ttggcctcca tggtgaagag gtggctttct tcgcgtccca
     8161 tggcccgcat gatctgccgc cagacatgga acatgctgtg tacggtgaaa cctgctgagc
     8221 cgcaggcggt atcgattacc gtctcgccct ctttcgggtt catcatcttg acgcacatgt
     8281 cgatgactca gcggggggta aaatactggc ccttctcgcc cttcgacgat ttcgagacca
     8341 gatattcaaa ggcgtcgtcg atcacatcga ggttggagtt gaatagcttc cattcttcca
     8401 gcgagccgat gcaaacttgc aggtggtcgg gcgacaggcg gatgcgttca tcgtcaagaa
     8461 agacgccagg ccatttgcgt ttggcgtcgt cgaacaggtt ctggatcgcc gccttcagtt
     8521 gcgccgctgt gttctgattg cggaatcgca gggcattgcc cagccggtgc gaattcatct
     8581 cgtcataaag cttggtaaag atcagcttga agacttcctc gaagacatcg accccggcat
     8641 tggccagaac ctcgtcttcc atgtcctcga tcaggtcgcg cagggagcgg gcgaacttgc
     8701 cttccttcac acgctcttcc tcaaggtcaa tcagggtctg gatggtccag ggctgatcca
     8761 cgacctgttc aatggtctga tctgcacggg gcagttcatg gatcggcata aaatagttgg
     8821 ggttcttgcg gtgccagact tcggccagga tgccgtcact ccacacggca aggggtgccc
     8881 cggtcgcgtg ggcataggac cgcagctgtt ccttgccgtc cttatactta gcctgcttaa
     8941 cctcgacgat gatgtagggg acggtcgggc ggtcggagtc gaacaccacg atgtcagccc
     9001 gcttggaact gtcgcgccca aaggtgatcg ggtattcgat ggcaatgcgc gaccgggcgt
     9061 agccaagctt gtcgatcagg cgagcgagcc aaagctggcg ggtacgttcc tcgggcttga
     9121 gttgcacttc cttgtcgcgg acgaggcatg ccacataagg cacctgcttg ccccgcgaat
     9181 cacgcaaaac tactcgggca tcaagcgctc gaacctcttc ttctgagaag agaacgaatt
     9241 cgctgaattc actactttgt tttttcattc aatactctaa attcgaattt ttaccattaa
     9301 cctagcccat cgcgaccaac tcctaatttg aagcggcgct aattattgag cgagataaga
     9361 atctatattt ttcagagaca ccacctgtca cgttgtctca tatgtcaggt attaatggct
     9421 atctttggca tcacaatgcc aacatgcttg taaagagtgg tgcgagagac gtcgtaacgt
     9481 cgcgccacat cagccacctg aatctgcggg ccgcgcaaga gcgctttgat ttcgcgcaac
     9541 tgctgttcgt ccagcttcgg cttgcggcca ccggcgcgac ctcgggcacg cgctgcggcc
     9601 aaaccggctt gcgtccgctc acggatcaaa ccgcgctcaa attcggccag cgctgcgaac
     9661 acatggaaga tcagcttacc cgccgccctg tcggtttcaa tcttttccat caaactttca
     9721 aacccgacgc cacgctgctc aaggtcggtt acgatctgca ccaaatcggg caggctgcgc
     9781 ccgagccgat ccagccgcca taccactagg gtgtcacctg cccacagtgc cttgcggcac
     9841 tgctcgagct cggggccgcg gtattctggc cactgaccgc ttcttcgtag atcacgccgc
     9901 actcaacctg ccgaagcgca tcgcgctgca ggtcaaggtg ctgatcatcg atcgatacgc
     9961 gagcatacgc tatgcgttga ttcatggaag aatccgggac attgaaaaaa ttgacaatga
    10021 tattaacact ataaggcggc atttacgacg gtaaagtgcg ccattttgat ctgtccaaaa
    10081 accgccgttt tacgaacatg tcggcgaatg cgataatatg aagtgaacct actgcaacgg
    10141 cctaggatta ggactcattg atcatagtca gaaaacaacg gtttctacga tacaccacca
    10201 gtaaggccga gactggtcac caatgatgag aagtaaaaag gcggccagtg accgcccttc
    10261 tttatttgcg cattacctca aaacatcatt agcacatgct tgacgtaggg ccctcatagc
    10321 ttcctgaaaa tcttccctag tcgccaggcc cgtttcggcg tcatccagtc ccacagcatc
    10381 taccgaagcc gtggccatct gccgcaacag aacggctagc tcggccgggt tgccctcgcg
    10441 gcggacctcg gtcagcagct ctactgcata aagcggatcg gcgcggaagc actcagccat
    10501 tgctgtgtcg tgcgatctgt ctttcatttg gtgccgtcct cctcccacca caataaagca
    10561 tgcagcaatc caagacaatt tgagagcctc gttttggatt gcacaatcca aaacgcagcc
    10621 cgcagacaat cagtgcccgg ccggtgcggg ccggcgtgag tcggcttgaa cgtgagccgc
    10681 agaccaagcg cttggatgac tttaaccacg gttgcgaagc tcagttcgcc atcgcctgaa
    10741 agggccttgt ccagtcattg gcggcccatt cccacgtcgc gggcaagctg gctaaggttg
    10801 cgggggcgga ccaagtctcc aaacgccgcc gcgatcagtg agggggtcgc cgtcctccgt
    10861 cacagcatcg agataggcgg caatgtcgtc ctcagtcttg agatagtccg ccgtgtcgta
    10921 gcggctgaat ttgacttcca tcgtgccact ccttctactg cgctgcaatg gtcttggcaa
    10981 atgcaatgcc ccgttcctag ttgcttttat cgcctccaca aagcagcacc aactcgcgca
    11041 ctgcttcaag atcggcccgt gctccgatcg gtctgttcag accaacggcg cgcagataag
    11101 cggaacgaga caagccagtt tggtcagcta gctctgtaat ggcagctttt tcctcatctg
    11161 ctacccagac ctctatgggc ttaccgcgcc gccgtatctt ttggtgccat cacttcgctc
    11221 atgatgatca actcctattg tgaacaatta cgcacttaaa cgcggcagcg tctgtgcgcc
    11281 tcgcagagca agatgccgtc gagccgtagg cgagtaaggg gcggtggtgg aggccctagc
    11341 tgcgtagcag cgtaggccta caacacacat cttgccgtac tgcttcatgc gattctatgc
    11401 gactggagca gaatggccag taaaatatgg cgtaatcgcg tacattcgcg ctaattcgca
    11461 tacattcgca tagcatcgca ttaatcaatt cttctaatcg catagaatcg catagattcg
    11521 cataaaaacg cgtttccgac tgagttcatg agcatcaggg acggactgac accaaaagga
    11581 gggggctttg agaggccatc agctaggcca gccaaatcta agtcttgtaa attgctcgac
    11641 tcgggtgaga acatagcctc cgtttacgcg ctttttttcg aaactcgatc atcaagaaac
    11701 aacggagtta tcatggcacg aaaaaccctc gcagagcgcc gttctgatgc cctttcagag
    11761 cttgaaatgg cgaaagccag attggcaaaa ctcgacaatg aggcagcaga aagaatcgga
    11821 agaattgcaa taaagtcagg attggttaat cttgagctta cggacgatca ggtccgggaa
    11881 gaattcgaca gaatcgtgga aaggatcagc aaggggaatt gatcatgaag acagctcacc
    11941 gcatcagcgc attggccaat caactcaacg aactgcaagc ctgcctcggt cgagccagtg
    12001 ggcggcctag caacagcgtt atggaagccc agcgcatagc ggccgagctg gcttcttcac
    12061 tcgaagattg gcaccttgaa acgctacaca ttcctgaacc ggagcgtgac ctctatcgcg
    12121 cacagaatcc ctattacgct gcccactgac gccgcgcact gccctatcaa aagctcactt
    12181 cggtgggctt ttttttgtcc gcaaaacgta tcaaaacgga caaccaagct cgttccgctc
    12241 gtcatgaaaa acgcgtccaa aatcatagtg ttgaacacca atcgcgggaa gcttgacgga
    12301 cagttacgga cataaaccat tgaattctat gcggatacga gattatccgc atatttaaaa
    12361 ctgctccata tcggtcaata atcaaatctc ggacgatgac aaaacgaggt attcggtatg
    12421 gaactagaat cgcaaaaaat gtcggcttat gccgcccgaa aattcggtcg aggcgcggcg
    12481 ctcgctcttg caatggcaag cgcaggcctt ggtgtaagtc tctacatggc accggctccg
    12541 gcctatgcca tctactgctc gaactgctcg acgttctatc agcagatgta cgagtacgcc
    12601 gaagcggtca acacgcaact gaacacggcc cagcagttgc agacgcagat ccagcagtac
    12661 gacaacatga ttaagcaggg catgtcactg cccagccgca tgttcaacac catcactggc
    12721 gaccttcagc gcgtggccag cgtctacaac tcggcgcagt cgctcggccg caacatcagc
    12781 aatctggacg aacagtttcg ccagcagttc aaaggctatg acagctatct gaactcgatt
    12841 ggccaagggc aaaacaacat gccgcagcgc tatcgggact gggcacaatc cggcttcgat
    12901 aacgcccgca cggccatgca ggccgctggc gtaaatacga gcacattcga ggatgagaac
    12961 gcgatgctgg atcagttggt gagccgttcg cagtcggccg ttggccgtca gcaggccatt
    13021 caagccggga acgagattgc ggcgcagaac gtgcagcagc tccagaaact gcgcgatctg
    13081 gtggccacgc aaatcaccct gcaaggcaac tacatggcca aacaaaccga acaggaagcc
    13141 gtgaaggacg cggccagtga aaggttctgg ggaggtacag acactcccgg cccatccaag
    13201 gggttttgat tatgacgatt tctaagattc ttcttttggt gttgtgtctc ggttctgctg
    13261 tgggtggggc cgtaatgact agcgtcgtca tttcttcggg cgaagaatgt tcggcttcac
    13321 agaagcaaaa agcggcggct accaaaaagt tctgggaagg gaaagacgaa cccggcccgt
    13381 cgaaggggtt ttgattatga gtagaccact ctttctaagt tgcgtcggga tagccatgct
    13441 gttgatgacc gggagtgcaa tggccgccgg tttacaaagt aatgtggaga tgaacgggct
    13501 gctaacagac attcataatg cgtctgctca gtgggggcca aagttgcagg gctatgccag
    13561 aaacttgctg ttcctgctcg ctgggattca gtttgtttgg acgttcggca acctgatttg
    13621 gcaaagcgcc gatgttggcg aaatgttccg cgagctgatc aagttcgtca tgacgatagg
    13681 ctttttcctt gccttgctga cgtactccac agagtggagc acggctatcg tgaacagctt
    13741 ccgcgaagcg ggatcgcaag cctccgggtt tgcgcaggag ttgatgcccg gtgacgtgtt
    13801 caccttagct atcaaattgg cacaaaaagt cctgtcgaac atttcgttgt ttgacctcga
    13861 aactgcggtt gtgattgcgc tatccggcgt catcattttg ctgtgcttcg cgttcatcgc
    13921 cgcgtttatg ttcgttacgc ttgtcgagtc gtatgtgatt atcaatgcgg ccgtgctgtt
    13981 catcggtttc ggcggctcac aatggacacg ggaatttgca atagccccgc ttcgctatgc
    14041 cgttgcagta ggcgctaagc tgttcgtcct gaccttgatt gtcggcctga ttatgtccgt
    14101 atcgaacagg tggttcgcca actacgacgg cactattggg ccaacattga cactattggg
    14161 cctgtccttg gtgtgcgcct atctgtctcg gtctatcccg gagctgattg cgggcatgat
    14221 caccggctca tcatttgggg gaggcagtgc gatcggcggc atggcaatgg cggccgctgc
    14281 ggcggctact ggcttggcgg caggcaccat gatggcgggc ggcgcaaacg cggccggtgg
    14341 tgctgcgggt gctttgggcg gcggttctgg tggtggcgct ggcagtggac tggccgacag
    14401 catcaatgcc agcatggccg gtggccagca gaaccccgca gcgtccgcaa tgccgtccat
    14461 gaacagtgcg gcgaccacag gaacggcaac gggcggtagc gcagcagctc agtcagccgg
    14521 ttctcgcgtc ggtggctcgc aggcgggcaa atcgccgtca ggtagcggca tcaacagcca
    14581 gagcgacact ccgcgcagca gtggtctgag tgggccgcag caggcggcgc aaacactgaa
    14641 aaaagcggcc tccggcgcga acaacttggc tagtgtggcc gcccgcggcc tcgggacgct
    14701 cgccgctatc tctgtgccgg gtatggaagg cgcggcgagc atgggcggcg gccccccacc
    14761 gcaaatgcct gacagcgatg agggtgacga aaaacgtcca gctaacagcg gtgtatccgc
    14821 ggatgttgcc gaagaaacaa acgttattcg tcccgcacaa gaaccgtccg ccgccccggc
    14881 tcagccgaac ggcgtggaga aatccaacaa cccgaaacag gaaggtgagg catgaacccg
    14941 tatagcaaac caatccccgg cacccttgct cgcatgaagg tgcccggcat gtcagcggaa
    15001 gcggtggcta aggctgacgc gccaatagcg gttgggaacc gccagagcga tgaaacccaa
    15061 tcaactgctg ccaaggcggc caatgcaccg gagactgacc aatgattgaa tatctgattt
    15121 tttgcgtggt ggccttcgct gccgccttcg gcatgtactt gctgcgcgaa tacctgcgct
    15181 cgcggggcta tgggccgcaa ctcgatgcgt ttgcgcggtg gctggaacca cgagagcaac
    15241 gcatgaaccg ttatcgcttt aaggctgcta atggcctgtt tggggcggcg cagtccatca
    15301 accgcctgcc cctgttcgga agcaaacatc aggtggtgat gatagagcaa ctgcgcatgg
    15361 ccatcatgtc cgaacaagca ctaagggagc gccagcagcg ccgcaaatga tctcgcccgc
    15421 cctgccaagt gcagggcttt tttatgggca ccaaaagaaa ggctccggcc gatccggccc
    15481 tacgtctcgc aaatcggctg taatgacagc gtaagcgctt ttatggtcct ccccgccacc
    15541 acaactctag ccccctctga aaacggctct ccgggccgcc tgcgaagcct gacactcgtt
    15601 tcaaagtagg ggcagccgcc cctgtgaaga taaccccgat acacggggca cagggtcagt
    15661 gggggcagcc gtctattaca caccgcaaaa acggcgtgta tcgcgctgtg agaagttttc
    15721 acacatgata gcaataaccc cattccgaca ctgaaaaaac gccgccacga cccatacggg
    15781 agggattctg gaacgctttc gcaggccgtt agaggcctta tgtgggtttt ggccctgccc
    15841 ttcccttttt ggtgcctgat gtctttttgc cggtttcaac ggctcgacca ggtgctgcga
    15901 ccggtgggta atccacggaa ctaaccgcgt cgacgacggc aggcccgccc tacctttttt
    15961 tggtgcccaa cggccgtagg ccgaccaaga gcagcaagcg atgagctgcg ccatcaaggc
    16021 gcggcgctta gcgcgcatgc tggagattct tagagaatct tggatggaat ttgcgaacgg
    16081 cccgcaaagg cccgccgttg ctgggttttc agaaactggc cgggtgccgt taagcagggg
    16141 gttgggtact gttaaacagg gggtgaggtg ccattaaaca gggggagcaa cgttacggtc
    16201 ggatgccttg ggtcgcccgt ttcccatttg cccttagcgt actcgtccag cctccaaccc
    16261 acggcagcaa gctcggccag cgcgttgggg cggagcgaac gcctttcatg agggagggga
    16321 gctgccgcga agcggtggga accccgcagg ggccacgctt ttggatcgtg gcgcgatttc
    16381 gcaccaatag gagttccgaa ctaacggatg ggggccatcg acttatgcac aggcaaaagg
    16441 gttttaaaca gttttatata tagttttaaa agatttagcg taaaaagaag gaaatttact
    16501 cttttaattc aatgaattac gcgcaatgcc ggttcctaat agacggatac cggttcctaa
    16561 tagacggcga aacggttcct aatagacggt ttcaggttcc taatagacac tctatccaca
    16621 gcgtggcggg ttgttcccac tttgtccgta aactgtccgt caatcaaaat gaaaaaagcc
    16681 cgcaacgggg ctcttttgcg atctcactca ccgtctatta ggaaccgccc agcggttccg
    16741 acgaggaatt gcggtgcctc atgaccagta gagggccatc cttccccgct tcttcgcaca
    16801 aggcatattc aggcaggtca tcggctttga tgatcccgcg aaggaagaaa cagaattttt
    16861 tgaacgagcc accgcttcca ctgcgttcgt aaagggtctt gaaagcccat ttagcgctat
    16921 ccctgcctgc ggcacggcga gccagtcggt agataaagcg tcctatgccc ggctcgatca
    16981 gaaagtagtc ccggtgtact gtaagtacct ccggccgctt gccctcggtg acttctcgat
    17041 aaatccactc cggggcttca atctcgacag aacttatctt cttggtgtcc gtgcgtgaca
    17101 gaacacgata gcgcgcgatc agcccctcgg cttcggttgt ccggaacttg ccgctttcgc
    17161 gcactgtctt gatggtagta ccccgcaacc gatctagcgc ggcttccact cccttaacct
    17221 gactactacc gttaccacgt cgggcaaatc tgaggatgtc gctaacatgc agccgaaaca
    17281 ctctccccgg cttgccaccc tttccttctc gatagcggtt catagcttcg gtcaagtgag
    17341 acaccatcat cagcacaatg tcgtagtccc aaacggaggc cattccgtgc gctcctgcgg
    17401 acacttccac gtatccatcc ggcaggtcgt agcggatcag ttctccggct cgcttgtcct
    17461 tcttggataa acggaacacg gccacatcca tgacgctgcg attgtcgcgg ccgcccacgt
    17521 cataaaggct gggcgcaaaa aaatctggct gagcgtcgcc ttgtggaggc ttgcggacaa
    17581 gtgacaacgc tttgtcccgc tcttttgccc tttccatctg ttcatgcacg gtcgagacgt
    17641 tagacggtct accgcgttca gcagccggag ttcggtcact gttccgcttc ctgatatttt
    17701 caaggcgttc cagtagcccg ccagagtcgc ctattaccgc gcctatgggc ttaagaccgc
    17761 tgtttcggct actcatgcac ggcccaaccg gagctgtatt aagcccagta caagcggtct
    17821 actcgtaaat gtcgcctgca agttggccgc cacaattcag gcccccattc cgggacgagg
    17881 gaccgagccg gaatgtttgc acgcatcaat ccatgcctcg acctcggcta aatcccacac
    17941 gaccttgcga ctactaagtg cgatgcgctt agggaatttt ccctgctttt caaagttgta
    18001 gatcgtgcgc tcgcacagtg gaatcatctc cagcagctgc tttttgctaa tgagaacttt
    18061 actcactctc cttaattctc tttccacacc gaatacctcg ttttgtcatc gcccgaggtt
    18121 taattattgc cccacaatgc cggtgacttc cagcagtata gagaactttt tctcaataaa
    18181 atcaacgtct tagtgtccgt aaattgtccg cctttcaaaa caaaaacctt cgcaattctt
    18241 tgtttttctt ggcactttat ttgacgcaga tagcgaagat tgcctgtcca tatggaatac
    18301 gttttacgga caaaaaaaag cccaccgaag tgggcatttc gctacaaaat gcaggtctca
    18361 agactccgaa tcagtccttg aggcggggct tcctgattgt ccactcgtca atcatgtccg
    18421 cccaatcctg tagcattgcc cgccgctgat cgcggtactc ggccttgttg tagacggctc
    18481 gaacgccctt ctgttcgtgc gcaaggcact tctcgatcca gtcagtgtta tagcctgcct
    18541 catgcagcag cgtgctggct gtacgccgca agtcgtgcgg tccgaacttg ccaagcgact
    18601 gcccttcctt ctgggccaga cgataggtca gcgtgagcac ctgattgagc gtggcgctgc
    18661 tcatcggcaa gtccgagtca tagcgcgatg gcagcacgta ctccgagccg cccgcgaagg
    18721 ttttcagagc tatgaaaatg tcgagcgcct gcctcgacag gaacaccaga tggggattgc
    18781 ggcgcttcat ccgctccttg gggattgtcc agagcgcttc ggtgaaattg atttcgctcc
    18841 acgttgcgtt ggtcagttcg cttttgcgta ccatcgtcag caacagcaac ttggcggccg
    18901 cgcggatcga aggtgtggtg ccaatgcgct ccatgtactg gtacatcaag ccgatttcat
    18961 cgggcgtaag tgctcgatct cgcggctcaa actttgcaat agtcgacggg cgcaccagat
    19021 cggccgggtt ctcaaccttc tggccgcgtt ctatagccca acggaagacc tgcatcacaa
    19081 tttcgcggca aagcacggcc gttgccggtg ccccgcgctc cacaatcgca tcagtcagcg
    19141 cccgtaaatc ctcatgggtg atttctgtca gcttctggtt accgaacttc ggcttaagct
    19201 cgcgctcgaa ggtagctcga cgcatatcgc gggtaacgtc cgccatttga tagccgcgta
    19261 gccatttttc gccccaagca ccaaagtttt cagcgtcctt aacgcgggct ttgtcccgcg
    19321 ccttctcttt ggctggagat ttccctgcgg cgatcatctt cttggcttcg cccagccgtt
    19381 cgcgagcttc cgctagcgtg atccctccga caccgtagcg gccgaaggtg atggtctcct
    19441 gcctaccgtg gatcgagtag ttgtagcgga atgaaatcga acccgctggc gtgatggcca
    19501 cgtagaggcc gtcacggtca ttcactttgt agagcttgtc ctttggcttg aggttgcgca
    19561 gcttggtatc ggtcagcatg gtcgggttag gtttcctctc aaaaatacca tgttcaaaaa
    19621 gccccacaaa ttctcaaaaa acctctttaa gaacaatgtg ttacctaaat tcaataccat
    19681 gaacacccca tcaggggcgg aatggtatcg agtgccgaac atggcatcag gttcaaattg
    19741 taccatctac catgccatag aaacggggtg cttggcgatg cgaaaatttg ccagacattg
    19801 ctacgccaag aacgaaaaaa gcccgcaatt atgcgggctt atgggtgttt taatgctctt
    19861 tggtgccagt ctatgccgga cgtgggatca ctcccactca atggtagctg gcggcttgcc
    19921 gctgatgtca tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac
    19981 gcgaccgagg aaatcgtacg gcagatgcgc ccagtgtgcg gtcataaagt cgatggtttc
    20041 gacagcacgc agagagacaa cccagtcata cttacgacca tcgcccatta cgccaacgga
    20101 acgtaccggc aggaacacag tgaacgcctg gctgactttg tcgtacaggt ccgctttacg
    20161 cagttcttca atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac
    20221 ttcacccagc acacgaacgc caaggcctgg tcccgggaat gggtgacggt acaacatgtc
    20281 gtacggcagg cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag
    20341 cggttcaacc aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt
    20401 gatgacgtgt gctttaccgg ttgcagacgc cgcagattcg ataacgtcag ggtagatggt
    20461 gccctgcgcc agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac
    20521 gaaaacgcga ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga
    20581 caggaagcga tcttctgccg gaacgtgaac aatgttcaga ccaaagtgat cgccaaacat
    20641 atccagaacc tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt
    20701 caggttttta ccgatagcgc ggtgcagtag cattgcggtt acggaggaat ccacgccacc
    20761 agagaggccg aggatgactt tatcgtcgcc tacctgctca cggatgcggg ctacagcatc
    20821 gtcgataatt ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg
    20881 ctccagcatg cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata
    20941 gaagcgtttt tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt
    21001 gacgaagtcg gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag
    21061 cggtttaccg tctgcggtca gcgcatcttc gataccgcga accagtgcgc tgtcgtttac
    21121 gacttcaacc tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa
    21181 ctgcatcgcc atggtctgca taccatagca aacgccgaat accggtacgc ctgcttcaaa
    21241 gacatactgc ggcgcacgcg gactgttttc ttcagtagta ctttccgggc cgccggaaag
    21301 aataatgccg cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag
    21361 ttcgcagtaa acgcccagct cacgcacgcg gcgcgcaacc agttgagtat actgggaacc
    21421 gaagtccaga atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga
    21481 ggcaagtgaa acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag
    21541 cccagacggt agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga
    21601 atgcccgcac cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca
    21661 cagccggtca ggcccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc
    21721 aggcgacctt tataggctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg
    21781 ttgtcactct ggaagtaacg gtcagaagaa cctttggaca tcgcacccag agagcccata
    21841 ccacggtaag atttgtaaga acggccctgg tagagttcaa tttcacccgg agattcttca
    21901 gtacccgcca gcatggaacc caccattacc gcgcttgcgc cagcggcgat agctttggcg
    21961 atgtcgccgg agaagcgaat accaccatct gcgataaccg gaatacctgt gccttccagc
    22021 gcttcaactg cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt cacgatacga
    22081 gtcgtacaga tagagccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc
    22141 agagcgcgtg cacctgcagc tgttgccacg ttgccgccga tgatttgcag atcaggatat
    22201 ttagcacggg tttcacggat acgctgcaga acgccttcag agtgaccgtg agaggagtcg
    22261 atcagcagaa catcaacacc tgcggcaacc agcgcatcaa cacgctcttc gttacccgca
    22321 cctgcgccaa ccgctgcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt
    22381 ttacgttccg ctttctggaa atctttcacg gtgatcatgc cgatcaggtg gaattcgtca
    22441 tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg
    22501 gcttcacctt cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc
    22561 tggttcaggt cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt
    22621 tcttcggtaa cgaccggata gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg
    22681 cgcagcgtcg tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt
    22741 ttcacacggc gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg
    22801 ataccgcctt cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct
    22861 gcggaaagca tagggatatt cagacgaata gttttcgtta gctgggtgct gaggtcagca
    22921 gtattcggca gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct
    22981 tctttagcga tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc
    23041 ggcattatac agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc
    23101 cccggagggc gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca
    23161 catgttacct tctcaattcc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg
    23221 tctgctgctt gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac
    23281 acaacccgct tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg
    23341 cgcgatgttc cgcaacagca accgccgggt gaccttccgt ccacagcatg ggcaacaagt
    23401 tttagttcgc gccaatatta cgctctacga gccgcgcggc gactaccaga taatcgttga
    23461 gagtatgcag ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa
    23521 gttgcaggct gaaggtttgt tcgatcagca atacaaaaaa ccacttccct cccctgcgca
    23581 ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt
    23641 aaaacgtcgc gatccttctc taccggtgat catctacccc accgccgttc agggcgatga
    23701 cgcgccgggg caaattgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt
    23761 gttgatcgtt gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg
    23821 cgtagcgcgg gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac
    23881 ggatgtgacc attgccgatt ttgttgccga tctgcgtgca ccaacaccgt cggctgccgc
    23941 cgaagtggtg agccgtaatc agcaagagtt actgcgccag gtacaatcga cccgtcaacg
    24001 gctggagatg gcgatggatt attatctcgc caaccgcaca cgtcgcttta tgcaaattca
    24061 tcaccgatta cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga
    24121 acgcctgcaa aaacggatga gctttgcgct ggaaaatcaa cttaagcgtg ccgggcaaca
    24181 gcagcagcga ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc
    24241 gcaaacgcgc attcagcaac tggaatatcg tttagcagaa accctgcgcg cacagcttag
    24301 cgccacgcgt gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc
    24361 aacgctggcg cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt
    24421 gaagcaagtt aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag
    24481 tgaagtaaaa aacatccagc cggtaaaaaa atcgcgtaaa aaggttcatt aagccagcac
    24541 gaattcaacg cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc
    24601 accgcaggct tttaacacct gcaacggttg atggcagtcc gggcaaagag ctttcatttc
    24661 aataaattcc ccgcaggatg ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg
    24721 acattgtgga caatgcagtt ccatatgaat tcctccggta gccattccct ctacattcat
    24781 agagggaatg gcagataaaa tacttacgga taattattta tttttcttga tgtgcttcat
    24841 cagacgctta cgtttacgca tctgggttgg cgtcagggtg ttacgcttgt tcgcatacgg
    24901 gttttcccct tctttgaact gaatacgaat cggcgatccc attacgtcca gcgatttgcg
    24961 gaaatagttc atcaagtaac gcttgtagga atcaggcagg tctttcacct gattaccgtg
    25021 aatcaccaca atcggcggat tataaccacc ggcgtgtgca tatttcagct tcacacgacg
    25081 accgcgtacc agcggcggtt ggtgatcttc aacagccatc gtcatgatgc gcgtcagcat
    25141 agaggtcccc acacgacggg tggagctgtc atacgcttca cgtactgatt caaacaagtt
    25201 accaacacca ctgccgtgca aggcagagat aaagtgcaca cgagcaaaat cgataaagcc
    25261 cagacggaag tccagcgttt ctttcacctg ctctttcact tcctggctca ggccatccca
    25321 cttattcacc acaatgacaa gtgagcgccc actattgaga ataaagccca gcagcgagag
    25381 atcctgatcg gaaatgcctt cgcgcgcatc aatcaccaac atcaccacgt tggcatcttc
    25441 aatggcctgc aacgttttga ttacggagaa tttctctaca gcatcggtga ttttgccgcg
    25501 tttacgtacg ccagcggtgt caatgagcac atactcacgt ccatcgcgtt ccattgggat
    25561 gtagatgctg tcacgcgtcg tgccaggcat gtcgtaaaca acaacgcgct cttcaccaag
    25621 aatacggtta gtgagtgtag acttacctac gttcggacga cccacaatcg ccagtttgat
    25681 cggcagactt tgcgggtcga agtcgtcttc ctcttcttct tcgccgttct cttccgcttc
    25741 aaattgcgcc cagtattcag cgtcttcgtc gacttcctct tgcggtgcga gatcttccat
    25801 ccacggcagc agcacatgct ccagcagact taatacgcca cgaccgtgag acgcggcgat
    25861 cgggtagatt tcacctaaac caagcgagta gaaatcaacc actgcctgat cgggatccag
    25921 accgtcagtt ttgtttgcca ccaggaaggt cggtttttca cgggagcgca gatgtttggc
    25981 aatcgcttca tctgccggca tcaggcccgc gcgcgcatcc accataaaca gtacgacgtc
    26041 cgcttcttca atcgccagca gcgactgttc cgccatgcgg gtttctacac cgtcttctgt
    26101 gccatcaatc ccgccggtat caatacagat aaactcacgg ccttcaattt ccgcacgacc
    26161 gtacttacgg tcacgagtca gacccgggaa atccgcaacc agcgcatctc gggtgcgagt
    26221 tagacggtta aataacgtgg attttcctac gttagggcgc ccgacaagcg cgaccacagg
    26281 taccatgttt aaagcctcat ttttataaat catcagacaa cgcacgctat attcgcgtcg
    26341 ttgttaaaaa caggaaaacg gcccctgtcc aggagccgtt ttcaaagtga acgacagaga
    26401 cgattaacgt gtaatagagt acacggttcc gtcttttgcc tggatcagca gtttgccgtc
    26461 agcggcaacc ggttcagtct ggaaaccgga actatcaact ttttgctggg caacgaaacg
    26521 accatcttcg acgttaatcc agtgcagata accttcactg tcacctacca ccaggttgcc
    26581 attatacagc accggagaag tcagcaggcg atgcagcaga tcgctttgtg tccacagcgt
    26641 aacgccgcca tcaatggtca acgccatcac cctgtcattt tgatcgacca gatagatgcg
    26701 attgccgtcg acgatgaaat cattcaccga acccagttcg cgtttccaca taatctgacc
    26761 actgcgcaga tcaagcgccg tcaggttacc attataggcc agcgcgaaaa caacgccgtt
    26821 aacaacgact ggagtcgtgt caacatcgct cagacggtca atttcggtag aaccggtcgc
    26881 ctgggaaata cgctgctgcc aaatcatctg gccctgttcc atcagcactg cgctgacgcg
    26941 accattatcg ccacccacga cggccgcacc aaaagccgtt gccggcgcag actcgccacg
    27001 caaagagagc gaaggcatat cgaggttaac tgtccatttg acagcgccgt cagcttcgtt
    27061 cagcgcttgt aactgaccgt tactggtgtg gattaacacc agaccgtcgc tgaccaccgg
    27121 gcgcgaaagt gcttcacccg cgactttagt ttgccatgcc acagtaccat cgctggtatt
    27181 cagcgcgtaa acctgcgcct tttcgctgcc aatgtagaca tgcccaccag acacggtcac
    27241 accgccagaa agtaatgcag gctctttaga gaaccagcca tctttctcgg ccaggctgac
    27301 agaccagatt tctttgccat catccgcatt cagcgctttt accaaaccag cgcggtccgc
    27361 tgcatagaca acgttgtccg ccagtgccgg atgaagattg gaatagaagt tgccaatgcc
    27421 gctaccaacg gaagtgctcc acgccgtggt cggcgtaaac tggttttcaa cggttggcaa
    27481 tggggacatc tttaccacat cttcttcgct gttaaacagc gaacagccgc ttaaaagggt
    27541 aacggaaagc agtcctggca gcagtaattt acgcaattgc atcgggtccc tctcagatgg
    27601 acaaattatt aattttcatc tgcatcattt cgctcagtgc cggagtaaca tcgcttttca
    27661 cgcctgcttc ccatgcacta cgcgcacctt gcttatcacc tttgctcagc aatgcttcac
    27721 cacgcaggtc ggcaacaatg gcagcccacc cttcaccttt gatgctatca agggttttca
    27781 gcgcggcatc agcctgcttg agctgtacct gaacgcgagc aagacgcaga tttatcacgg
    27841 ctttgagatt ttcatcgctc gtgtctgcca gcccctgttg taactgggcg gcagctttct
    27901 ctagttcatt tttgtcaaca aattgctgcg caagttccaa agaagccagc gcaccataag
    27961 tatttttatt ttcagcagca aatttttccg ccgccgggat gctatccggt ttgccttcgc
    28021 tcactgcggt aatcgcattt tgataggcaa gagaagcgga gcgtgcagaa tcaacctgat
    28081 ggctgttcca gtagcgccag ccaatcagtg cgccaacgcc caaaatcacc ccaacagcca
    28141 gtgctttgcc attttcagca aaaaagcgtt taaccgcttc tacctggtcg ttttcgttct
    28201 cgtaaatttc cacgctgtcc ttctccttcc ttaacccagt aacgtgcgca aatgcgcggc
    28261 tacgctatcc tgcgcaactg ccgtttgctc accagagcgc aaatccttca ctactgctgt
    28321 gccgttagcc acttcagact cacccagcac cacagcaacg cgggcacccc atttatctgc
    28381 acgggcaaac tgtttcttaa agttgccgcc gccgtggttg gtcatcaatt tcacgcccgg
    28441 taattcatca cgcagacgct cagctaatgc catagccgca gattgtgtat cagcacctga
    28501 agccaccagg tatatatcga caacaggatc ggctttaaat tccggattaa cggcctgtac
    28561 taacaataca agacgttcga ggcccatagc aaaaccgaca gccggtgttg cacgaccgcc
    28621 cagttgttcc acaagaccgt cataacgacc gcctgcacac acggtgccct gggagccgag
    28681 actgttagtc acccactcga aaacggtacg gttgtagtaa tccagaccac gcaccagacg
    28741 ctggtttacg gtgtaagcga tccccgcgct ctcaagcagt ttgcacagac cggcaaaatg
    28801 ctcacgagat tcctcgtcca gataatcacc taatgccgga gcgtcgttga gaagcgcctg
    28861 cacttccgga ttttttgaat ccagcacgcg cagcgggtta gtgtacatgc ggcgtttgca
    28921 gtcttcgtcc agcttttctt tatgctgctc aaggaatgcc accagcgcat cgcggtaatt
    28981 ggcgcgtgct tccagcgaac cgatagagtt cagctcaaga gttacgtgct cggaaatacc
    29041 cagcgcgcgc caccagcggg cagtgagcat aatcagttca gcgtcgatat ccggaccttg
    29101 cagaccgaaa acttcgcagc ccaactgatg gaactgacga taacgccctt tctgcggacg
    29161 ctcgtgacgg aacatcggcc cgatatacca cagacgctgt tcctgattgt acagaagacc
    29221 atgctcgatg ccggcgcgta cacagcccgc cgtcccttca gggcgcagag tcaggctgtc
    29281 gccattgcga tcctcaaagg tgtacatctc tttttcaacc acgtcggtga cttcaccaat
    29341 cgcacgtttg aatagcgggg tctgctctac aatcggcaag cggatttcac tgtaaccgta
    29401 gctgccgagc acgtttttca gtgtgccttc aatgcgctgc cagatggccg tttcgccagg
    29461 caggtaatcg ttcatgccgc gaatggcttg aatgtttttt gccacgttta ttctctttct
    29521 gaatataaaa atgaaccctc aacgcttccc tcaatgtttc gggagccatg cgggttcaat
    29581 catacacggg aagcgaggcg cttcccatca cgttattatt tttcaacctg ctgaacgtca
    29641 attcgacgcg cttcatccag ctgactggct ttcgcacgaa tgcgtgcctc cagctggtcg
    29701 atcatatcgt tgttgtccag acggtctttg cgcacgccat cttcatagag gccgcttttc
    29761 ttgttgccgc cggtgacgcc gagtgtagaa accagcgcct cacctgggcc attcaccacg
    29821 cagccgataa tcgaaacgtc catcggagtg atgatatctt ccaggcgttg ctccagcgcg
    29881 ttaaccgtac cgataacatc aaattcctga cgcgaacagg tcgggcaggc gatgaagttg
    29941 atccctcgcg aacggatacg cagcgatttc aaaatatcga aaccgacttt gatctcttcg
    30001 accggatcgg ccgccagtga tacgcgcagc gtgtcgccga tgccttcaga cagcagcaga
    30061 cctaaaccaa tggcggattt tactgccccg ctgcgcgcgc cacccgcttc ggtgatcccc
    30121 agatgcagcg gctgatcgat ttgttttgcc agcaaacgat aagactcaac agcgaggaag
    30181 acgtcagacg ctttcacgct gactttgaac tgatcgaagt tcaggcgatc gagatgatca
    30241 acatggcgta tggcggattc cagcaacgcc tgcggcgtcg gttcgccata cttttcttgc
    30301 agatcttttt ccagcgatcc ggcgttaacg ccaatacgga tcggaatgtt tttatcgcgc
    30361 gcacagtcaa ccaccatgcg aatacgctct tcattaccga tattgccggg gttaatacgc
    30421 agacaatcga cgccgtattc cgctactttc agcgcaatgc gatagtcgaa gtggatgtca
    30481 gccaccagcg gcacgttaac ccgctgtttg atgagtttga acgcttctgc cgcgtccatc
    30541 gtcggtacgg agacacggac gatatcagcg ccaacgcgtt ccagcgcctt gatttgattg
    30601 accgttgctt cgacatctgt cgtacgcgta ttggtcatgg actgtacggc gatgggagca
    30661 ccatcgccaa tcggcacatt cccaacgtaa atacgtgttg attttctacg ttgaattgga
    30721 gcctggttat gcatgaaaaa tctcccgcgt tacccgtctg ttactgcgcc ggtgattgtt
    30781 cggcattgag ggtcagacgc gcaacctggt tagttctgat aaaacgactc agatcgacag
    30841 gtttcccttg atactggatc tgtactgcgg ctggcgcacc aattttcagt ttgtacggtg
    30901 cctggccggt taagttcaaa ttaccgtctt tacgttgcat accgctaaac aattttttac
    30961 cggtagcatc agtgacctcc agccagcaat cggcagtaaa gttcatcacc agcgcattcg
    31021 gatcagccgc cggcgtagtc acgcctgcct gatcggttgg caacggcgca gcgccatctg
    31081 gcgttgttgt tgccgttggt gccggggtcg cggcggtatc aacatttgcc tgcgaaggcg
    31141 aaacaaccgc attctgttgc ggatcaacag ctggtgctgg cgcagttacg gcaggtgttt
    31201 gcgtgttggt tgcggtagta tccacagacg ctggcggcgt ggacgttgta gctgggtctg
    31261 tagttgtcga cgtatttaac ggaacactct gcccctgctc gctattactg ctcagttccg
    31321 ccgaagattg atcggccata gtggtgatct cttcctgctg agctttgtgg tcttgccacc
    31381 accaggcacc gctcaggccg ataaccacaa acaacaccag ccaggtgaag gtcatcagcc
    31441 agccgtcgcg ttttttgcgg cgtttaccga gggaaaaact ctgcatcggc gcaacttttg
    31501 cagcccgtag tggagcctgc ttttccagcc ctggcagcag ttcttcttct ggaatatgta
    31561 ccagacgcgc ataagagcgg atatatccgc gcaggaatgt tgaagcaaga tcggcgggtg
    31621 ccttatcttc ttcaatgtcg cgtaccgtgg aaaccttcag gcaaagtcgc tcggcaacgg
    31681 cctgctgact aagtcctagt tgttcgcgag cattacgcag gcgagcgccg gtagtaagtg
    31741 cttcattttg gtcgtgcgtg gcttcagtat tcattcgctg caggtacgtt taaatgagaa
    31801 ttgagatgcc ggtgaaccat catgctcacc cacaccgcga aacatccgtt aagttaaccg
    31861 ttatcataca gtataagacc gtctgtgtgc tcgtgacaaa gcccacattc actaaggcta
    31921 acttactgtt gcatcgttac atactgcctt aaagtcagca aaaacgcacc gttaatattg
    31981 accagacaat tgcaacttaa tacttcattc ataaaacatt acgccacggt acataaagta
    32041 accgtggcgt aatggctatc agaccgcttt aatgtcgatg gcttcaccct gcatccgttt
    32101 acgcagggta cgtttcgtac ggtcgataac atcccccgcc aactgaccac aggcagcatc
    32161 gatatcatca ccacgcgttt tacgcacaat agtggtgaaa ccgtagctca tcagcacttt
    32221 tgagaaacgg tcgatacggc tgttcgaact gcgtccatac ggtgcgcccg ggaacgggtt
    32281 ccacgggatc aggttgatct tacacggcgt atctttcagc agttccgcca gttggtgagc
    32341 gtgttcagtg ccgtcgttaa cgtggtcaag catcacgtat tcaatagtga ctcggccttg
    32401 attggcgttg gatttctcca gataacggcg caccgcagca aggaacgttt cgatattgta
    32461 ctttttgttg atcggcacaa tttcgtcacg aatttcgtcg ttcggcgcgt gcagggaaat
    32521 tgccagcgca acgtcgatca tatcgcccag tttatccagc gctggaacta caccggaagt
    32581 ggaaagcgtc acgcgacgtt tagacaggcc aaaaccgaaa tcatcaagca tgatttccat
    32641 cgccggaacg acgttgttca ggttgagcag cggctcgccc atgcccatca tcactacgtt
    32701 agtgattggg cgctgaccgg tgacttttgc tgcgccgacg attttcgccg cacgccacac
    32761 ctggccaata atttccgata cccgcaggtt gcggttaaag ccctgctggg cggtggaaca
    32821 gaatttacac tccagcgcac accccacctg cgaagagacg cagagcgtgg cacggtcgtc
    32881 ttccgggata tacaccgttt cgacgcgctg atcgccaacg gcgatcgccc atttaatggt
    32941 gccgtcggat gaacgctgtt cttcaaccac ttccggtgcg cggatttccg ccacctcttt
    33001 cagtttgccg cgcaacactt tgttgatgtc ggtcatctca tcaaagttgt cgcagcaata
    33061 gtgatacatc cacttcatca cctgatcggc gcggaagggt ttttcaccta aatctttaaa
    33121 aaactcccgc atctgctgac gattgagatc cagcaggttg atttttccat ctttggtagt
    33181 gacgttttca ggtgtgacta attgttcaga catatgctat tccggcctcg ttattacacg
    33241 ttatggcccc tggagggttg aaaaaagaaa cgccccggtg agcggctgct cgtccggggg
    33301 cgctgcattg tacaaattct ggcgcacgga tgccacgttt gcacgcggca tttacgaaat
    33361 tattaacggg tgcgcgggca cacttcgcct tcgccgaaga aataagcgat ttcgcgagcg
    33421 gcagattcga cggagtcaga accgtgggta ccattttcgg tcaggctgtc agcgtaatca
    33481 gcgcgcagag taccggccag tgcgtttgcc ggattggtcg cgcccagcag atcgcggtga
    33541 cgctgaacgg cgttttcacc ttccagcacg gaaaccacga tcgggccaga ggtcatgaat
    33601 tcaaccagac catcaaagaa cggttttcca tcgtgttcag cataaaagcc acgagcctgt
    33661 tcaacggtca ggtgcagcat tttggtgccg acaattttga accctgcagc ttcaaagcgc
    33721 gcaaagatat taccaatgac gttttttgct accgcgttcg gtttgataat ggaaaaagta
    33781 cgttcaatag ccatttttac ctctgtaaat tgttctgttg ttgtctgtac cagcgtacga
    33841 gatggcgcgg attataatga gcaacagagc cgttgactat tgatggaggt aacatttttt
    33901 taaaataaac ctagtttaag caacaatcag ggacaaaaca gactattgca tgacaaattt
    33961 cactgacgcg atttgcccta cgtcatccat caccagcaat tgataatcac ctttatccgt
    34021 caaatgcagg gtcacgttgc gcccgcgttc agttaacggt tcgccattca gaaaccacca
    34081 gcgttcacct gcccctccac ttgattgcaa cggcaaagtc gcttccgctg cgcccggtaa
    34141 acgtttaata atcgcgccat cgcgaacgcc tgtcagctgc agcgggagct gggcatcgtg
    34201 accgtatggc gggcaacttg tcgaggccgg tggtaagcgc acagcgcgac gttctgatgc
    34261 gggcagccag ggttccagcg gtagcggcca gacatttatc atttcttgtc gcgcttgcgg
    34321 gcaatcagcg gcaacacgtt tgccattttc atccagccag atggggaaac gaatgccatt
    34381 aatgccttcc tgctccggca gtaatagagt tggcggctga ctcccgtcca gcagccaggt
    34441 tgccaggcgg cggcgacagt taccgtcacc ttccggcaaa gactgtccgc ccggccagca
    34501 gataacgcca cgagtgactg agttcgggcg cgggtcttcc ggcagattcg cactgcgcga
    34561 cagtaagata ttattgacct gattcaacaa tggtacggca ctggcaaagc caaactgacc
    34621 aacaacgggc gtgccgtccg gtctgccagt ccagatccca atgacatagc gagcgttaac
    34681 cccaatcgcc caggcgtcac gatagccata gctggtgccc gttttccatg ccagtggggc
    34741 gacgcgcggc aaggcactat ccggcaaggg ttgcgcttca tcagccataa tccggcgaat
    34801 gatccacgca gcccccgagg acattaaagg ccgttcaagc agcggatcgt caggctgtaa
    34861 gcgcaatttg ccggccttgc cgtggcgagc aaacgcggta tacgctgccg ccatatcttc
    34921 cagttttgca ccagcgccgc cgagaatgag cgaaagattc ggcgcagcac cgttgggcag
    34981 atacaacggc aatccaacat tgcgtaattt tgccgcaaac cgtttcggtc cataggcttc
    35041 cagcacctgc acagcaggta aattcagcga gcgcaccagc gcctcgctca tgctgatcgg
    35101 gccatgaaaa ccgctatcaa agttacctgg tcgataatca ccggtgcgcc gggggacgtc
    35161 ttgcagcagt gatgccgggt ggatcaagcc ttcatccagc gccagaccat aaacaaacgg
    35221 tttgagcacc gatcctggcg atcggatcgc attgaccata tcgacatgac caaagcgtga
    35281 atcatcgttg agatcaaccg atcccaccca gccgcgaaca cgcatatcgg tatgatcaac
    35341 cacgatcatc gccagtgagc tgcgcggtgg taatcgtcct ttccagtttt gcgccagttc
    35401 ttccagacgt cgttgaagac cggcatccag cgtagtaacg attttgtcgc ttttgctttt
    35461 accgagcatc atgcgcgaaa acagcggtgc cagttgcggc atttgtcggg gtgccagcca
    35521 gatgggttct tcccttgact cttttacctg ctcacgggac cacacacctt gcgcagccat
    35581 ccgttcgagc actttattac gcgcggcttc ggcacgctcc ggccaacgat ccgggcgaag
    35641 acggctgggc gcttgcggca aaaccgccag cattgccgcc tcggaatagc ttaaattcgc
    35701 aggcgatttt ccgagataag cccaacttgc cgcaccgatc ccctgcaacg taccgccaaa
    35761 cggagcgcgg ttaagataca aggtcagaat ttcacgctta gacagatgcc attccagttg
    35821 caacgcgcgc cagagctggc gaattttgcc gccaaatgtt ttggggtgag gatcaagcag
    35881 acgagcaacc tgcatagtga gcgtgctgcc accggaaata acccgtcccg aagtgagatc
    35941 ttgccatgct gcgcgcgcca ccgagaatgg attcaccccc ggatgcttcc agaaccagcg
    36001 atcttcataa ttgatcagcg cttcaaggta acgtggagaa acatcttcga ttgttaccgg
    36061 ataacgccag atgccgtcag catcggcgaa gcgccagagc ggcgtaccat cctgcgccac
    36121 gaccactcgt gcgggattga cttcatgcag cggtagaggc cagagtttat ctgctcccca
    36181 cgctgcaaga aaaaggagaa agggcgcagc tgccagcgtt atccagcagc cgcgtttggt
    36241 tgacaagcga ggcatttacg gtctgacaat cagcagatct tcagccgcgc cggtcgcccg
    36301 ccattgggga acatacattg attccaccat cggttgcggt acctgatacg ttcccggcgt
    36361 caccgcccgc gccagataca ccagcgttac cggttggtat tcatcaacgg caaccgccgc
    36421 cacaaagcga tcgtcacgga actcaatgtg cttaatactc gcctgctgca tctggttcag
    36481 taagttctgc acttcgccac cactttgctc caggctggcg ctaccgttcg ccagattctg
    36541 gttttccagt tccagacccg caggcagcag atccacgacc aacgcatccg gcacgctgtt
    36601 actggctttt acctgcaacc acaccagcac cagatcgccg ctacgtaacg agtccagcga
    36661 tttgctctta ccatcagtac caagaatatg acgctcgatt tgcagcacat tgttcgccgg
    36721 taaaggtgcg gattgcggat aaccgctggc atccacacgc agccataacg gctgatcgcc
    36781 actgttggtc acctgcaagg tagcaagttc atcgctattc agattactgt tttgcgcttt
    36841 ctcgcctgtc agctgctcag ctgagaaaga ggtttgcgcc tgccatttac cgggtaaatc
    36901 ctgaatcgta cgggcagcca ggaacaacgc gttactttcc tgcgtcgata gccagcgttc
    36961 accaaacgcc tgctgcgaaa gtgtgttcag caaagtgtac tgctcatcgg gtagcagttt
    37021 attttcttcc agcaaggaga gcattaacgc gttgtcgcgc agtggactac cgtaatcacc
    37081 cagccatatc cgctcatcac tatttctcgg cgttttcagc gccagcgcaa tcgcttcttc
    37141 accacgcgtc gcatcaccca tggttttcag cgcaacgcca agttgcagca gcggtaaacc
    37201 agaagcggca tctgcacgat gctcccagat ttcacgcagc gcacccagcg gagccttttg
    37261 ctgacgggcc aacaccagcg cagcgtaaga ctgtacggcg aatttactgg ctttgagatt
    37321 atccgcgtac gggatcgaca tcatgcccgg atcttgtaaa tagcgcagca gacgctcatt
    37381 accccggtta atggcgtctg tcggcacgct gtaaccctgt tcgcctgcgc ggaccaggaa
    37441 atccatcacg taagccgtca gccagtactc ttcgtcaccg tttttatccc acagcgcaaa
    37501 gccgccgtta tcacgttgca tttgcagcaa acgggaaatg ccgatatcga ccgatgcacg
    37561 gcgtttctca tcactgtcgc ctttgatgcc caacgcctgc agttgggctg cgttggtata
    37621 aagtgacgga aacaggccgc tggcggtttg ctcaagacag ccgtacggat acgcttttaa
    37681 ctctttgata taacgtgcga tgttcagcgg tggtttgccg ctcaacaaca attgcccttc
    37741 cagcgtaaca ggcgagaagt tttgcaatcc atccgccgga atcgcccatg tctcaccagg
    37801 ctgtaacgcc gtaccgtaat taaccgtttg tgccgggaac gccggacgga cgccgatttt
    37861 ccactgctta tgctgatcgg caacggtttc acccggtaac gctaacccgc taatggtggc
    37921 ctgaatttct ccatcgccat aacccggcaa tgctcgcacc gggataaaca gcgtagtacg
    37981 cacacctggc gctaattcaa cggctgcggg tgaatcgctg accagttcaa gcaaaccact
    38041 ggcggtcagg gcaacgttca gtttttgcgg tttatcggta agattagtga tatccagcgt
    38101 cagacgcgag gtatcgccac tcgccataaa gcgcggcatg ttcagttcag caatcaccgg
    38161 tgcggcaact atcactttac tttcgttgct accgaagtca tctgccgtcc aggcttgcgc
    38221 catgacgcgc aattcaccgt taaaatcgcc aatcggcagt gtaaccgagc cttcgccctg
    38281 ttcgttgagc gttaccggca gtgcctgctg cgcgacaata ttgatgtgat tgaccggcgg
    38341 tttaccacca cgtttcagct catcaccatc gccaccgaaa cgcagagctg ccagacgccc
    38401 ctgaccttca ataacctgac cgtaaatatc gtaaatgtct gcgccatagc gtttctgacc
    38461 aaagaacgcc tgccacggat ctggcgtgac gtagtcagta atattcagca caccactatc
    38521 aacggcagac accagcacat tcacctgttt aggcttctcg ccatttttag tgctggcttt
    38581 aattttcacg gttaatggtt gattgggacg catttttgct ggtgtttcca gcgccagatc
    38641 gaggcgacgg ttttcatcac caagcggcag atgcaacacc ccaaccgcgc gttttggcgt
    38701 cgcggagcga gatttatcgc caggacgcac caccagcgta ctcaaataaa gatcatgacg
    38761 attccaggtt ttatcgaccg gaatcgtcag atccagccct tgagccggaa catcaatctc
    38821 ttgccaccac agcggccctt cactggactc gaccatcgca taacctttac ccgccgttgg
    38881 cgcggcaata tgcaacttaa tggtatcgcc agggcgataa ctggctttat ccagtttcag
    38941 cgtgacacgg tcgggtcgca cggcaccgct cccgtcgctg ttgtcctgcc agctatagcc
    39001 agcccagaaa cgaacactac tgaccgcttc attcggcgct ttgacttcca gacgataagc
    39061 accccactct accggaaaac tgaccttgcc ggtttcgtcc gctttcagat ccagagtttg
    39121 ttcattttcg atcagatctt tttgatcaaa ctgtgactgc cagccttcat cttctgacca
    39181 gttccagtag taatcgcggc gttcgcgaat caggcgcacc tgcaagcccg acacggcttt
    39241 tttcactcct tgcgcatcgc tataaacgat ttcaaaagcg gcgttactgc cttcatcaac
    39301 aatgggctgt ttcaccgtgc tgtcagtacg ataatcgtaa accgatttcg aggcgaactg
    39361 cggacggatc cccggcaatg catcggcagg ccagatagcc tgctcagcgc ggcgcgtcac
    39421 cgggcgaccg cccgattcca gcaaactacc ctggaaaata acctgtaatg gggaatgcgt
    39481 ttccttccac tggctttctg tagaaacttc gccgcgccct ttatcatcca gcgtcaactg
    39541 aacttcatcc agcgtgcggg aaaggttttc ggcagctata tcgccgaatt caaaaccagg
    39601 taaggctgac acagcctcac gcagtgggcg caggaaaagt tgcccttgca aagtattacc
    39661 attagcaggc gcaccgtaca ggtagtatcc caccacggag aatttcactt catcattcgg
    39721 cgtaagcggg gttttctcac cggtcagatt cagcgccata cgctctggca taaaatcttc
    39781 gacgtggaaa tcccacatcc gatactggtt atcgcccgtg ttagcgcgaa tatgccacat
    39841 accggttgcc gcattgctat cgagtggcca ggttaagtgg tagaggccat tctccggctg
    39901 actaacgacg ctcctgagaa cctgcccgtc gggtttaatc acgtctaact tgatgggttg
    39961 atcgggcaac gctttaccgt ctgcatcacg cagcaaacca ttgaggatta ccgtttcacc
    40021 cgggcgataa agatcgcgcg ggccaaacat gaaaaactgt ttgctatagc ctggcgcgcc
    40081 agcaatgtta aattctgcta agtccagcgc cggaagtttt aaatcgagta gcgttgtctg
    40141 accgtcttta cgcgccaaca gtaatgccgc gtttttatca ttttccagct gcacatgccc
    40201 ctgagcgtca ctggttgcct gagtcagagt ctgccctttc tcatttaata aagagacttc
    40261 aattccttgc tgggccgcgc cgttttccag actttgggta aagatatcca gacgattgtg
    40321 ataacggtga gctgaaacgc cgatatcact taacgtaaac agcgtcgcgg gattactgta
    40381 atcgtaacgt ccagcctgat tcatcacagc cagatacacg cccgcctgct gaagcggttt
    40441 gatatcgccc agcggcagca ataatttttc acgggtgtta cgcgcaggat tgagatcaaa
    40501 ccgtccggtg tagaccagat ccgccatctg cagcagtttg tctgactgcc agttcgccag
    40561 cgaattgcgg tattcccatt ggctaatgaa tgctggcaga gattctggct taacgcgaaa
    40621 gaagttaaca tcgacattat taacgttgag cgccattacc ggcagccctt caacgacttt
    40681 accaggcagc agcgaaccac ggctggcaaa accgacgctg ggttggatgt cgcgggtagt
    40741 tatagttttt tcgtaatctt tactgaaagt tgcgttgttg agtgctttga cctccttgcc
    40801 aatagtaacg atcaaatcac gtttcggttc gaggtggcgt aaacgcagct ctttaagatt
    40861 atctgacagc tcccaggcac catccacttt gccgcttttt ttatcgacga catgaataac
    40921 gcgtgagaaa tcctgatccg ggtcgagagg gatggagaac gtcagcacca gcgtggctgc
    40981 accatcaagt tggacttcag agagatccag caatgtcagc gccttaccgg cactctgttg
    41041 tgccagtttt tgtcgctctg gcgcggagag ttttgctgaa ctcgcgtttt ctgaagaggc
    41101 cgctttagta acttcagaag gcgcatcttt tttcaccgct gttggcgcgt tatcgttgtt
    41161 gtcgcaccct gccagcgcca gcattagcat gcaggcggct acgcgtaact ttttcatttt
    41221 tcatccctgc cacaatggcc cgttagcaac gtcgaataat tattatgcgt gagaatttac
    41281 gttatgtaat tcagtatcac gcccatacaa tccgaatttt agtgagaatt gtggctcaga
    41341 cagccaaatc agcctctttt gcttgtcgct tttcactaaa catccgacaa tttagcctaa
    41401 ccccggcaaa aatggagatg cctatgtcca caacatggtt tgtaggagcc gactggctcg
    41461 ccgaacatat tgatgacccg gaaattcaga ttatcgatgc ccgcatggcg tcgcctggac
    41521 aggaggatcg taacgttgct caggagtatc tgaatggaca tattcccggc gcagtgtttt
    41581 ttgatatcga agcgctttct gatcacactt ccccgcttcc gcacatgctg ccgcgcccgg
    41641 aaacgttcgc cgtggcgatg cgtgaattag gcgttaacca ggataagcac ctgattgtct
    41701 atgacgaagg taatcttttc tcagccccac gagcatggtg gatgctgcgc acctttggtg
    41761 tagagaaagt gtcgattctg gggggtggac ttgcaggctg gcagcgtgat gatctgctgt
    41821 tagaagaagg tgcagtagag ctgccggaag gagagtttaa cgccgcattt aatcctgaag
    41881 ccgtggtgaa agtaaccgat gtgttattgg caagccatga aaatacggcg caagttattg
    41941 atgcccgccc ggctgcacgt tttaacgcag aagttgatga acctcgccca ggtttacgtc
    42001 gcggacatat tcccggtgca ttgaacgttc cgtggacgga actggtgcgc gaaggcgaac
    42061 taaaaacgac cgatgaactg gatgcgatat tttttggtcg cggcgtcagc tacgacaaac
    42121 caattatcgt cagctgcggc tctggtgtaa cggcagccgt ggttttgttg gcactcgcga
    42181 cgctggatgt gccaaacgtg aaactgtacg acggcgcatg gagtgaatgg ggcgcacggg
    42241 cagatttacc ggttgagcca gtgaaataag ctttatacag gcaataaaaa accgccgaat
    42301 ttggcggttt tttattgctg gtctggttcg cggcctttcc agcaggttgt attaccgtag
    42361 taatgcaagc gcgtctcagc ggagacaata ctcgccagta actctctttt tgtcaagcaa
    42421 aagagagtta ttattgttct gttagtgtat tatccactgc ggccctttcc gccgtctcgc
    42481 aagcgggcgc tggcttaagg aaaggatgtt ccgcgaccgt tagagcaagt gcgtctcagc
    42541 gcttgctatc gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc gcatggaccg
    42601 cacaatgaag atacgatgct tttgtatcgt acttattgtt tctggtgcgc tgttaaccga
    42661 ggtaaataat aaccggagtc tctccggtga caatttactg gtggttaaca accttcagag
    42721 cagcaggtaa gcccgaatgc cgccctttgg ggcggcatat ttttagatta tccgattctg
    42781 tttaaagtca cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt
    42841 aataaagtgg ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc
    42901 gccaggtaat gtatcggtcg ctacacttcc cgccacctga ataatttctt cgatatcacc
    42961 atcggcttca atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca
    43021 aataaaagca cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat
    43081 ttgtgctggc ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc
    43141 ctggctgctc agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa
    43201 ttctttaccg gttggcagtt tggcattaag gaagagcgtt tcgccaagtg tcatctcaaa
    43261 cagcgtgcga acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagtgc
    43321 ttctaacgag gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa
    43381 atcaagcgcg ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac
    43441 ggtggatccc agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt
    43501 ttccagcagg tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac
    43561 gttcggggcg caaatgcctg atgcgctacg ctaatcaggc ctacaaggga ttcgcaattt
    43621 gttgaatttg cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt
    43681 ttacgccgtt aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca
    43741 ctgttcaacc ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg
    43801 atagttctca acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc
    43861 cgcacttccg gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaactc
    43921 cgccagcggc agacgccaga atggttcgtt ctcttgtgag gcactcgcca gcaagcgatc
    43981 ggcaagcgca tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cggttttcgc
    44041 cgccccggtg agggtcgccg catcaatgat cagttccggt ttctgcgcac tggcatcaat
    44101 cagaccatcg gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt
    44161 accgttgcga taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc
    44221 cgcacagcag aggaacagct tcacgcgctt gttcagtccg cgcgtaatgg caaatgccag
    44281 cgccccggta accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt
    44341 ctgtttgatg ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta
    44401 cactggcgct tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg
    44461 ttctgaaccg cgtccgactg tgtgcagccc catataacct tgctcacgca gatcttcgcc
    44521 tttggtgatc cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg
    44581 ctgtgccagt tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca
    44641 gtcgatgatc atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca
    44701 caccacttta cgcgtgcctt tcggggcttt gtaaccttgc cagaatgccc agcagcgatc
    44761 cgcatcccag ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg
    44821 cgccgcacgc tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc
    44881 attattaatg ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag
    44941 ggtaatcttc atcgcttttg tcattttagt tatccttctt tgtaaaaggg ccgccagttg
    45001 gcagccctag gatttttccg ctaaatgatt cgcgttgcag gaaggcggca agtgagtgaa
    45061 gccccaggag catagataac tatgtgactg gggtgaacga gcgcagccaa cgcatctgcg
    45121 gcgtgaagca tgacgcggaa attattcggc ctcgtccagc cagactaaca aaatcgcttc
    45181 gaggattttc tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca
    45241 ctgatgcata tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg catcgtacag
    45301 tgcttcgcca atttcgcggc tatcggtcca cttaagtccc atacaaacct ctgttaatgc
    45361 tcacgcgcat ggttgatagt gtaacgcggg atttcgacca ctaaatcttc gtcggtgacg
    45421 cgcgcctggc agcttaaacg gctttccggc tccagccccc aggctttgtc cagcatgtcg
    45481 tcttcctgct ctgagctttc cggcagtgag tcaaaacctt cacgaacgat gcagtggcag
    45541 gtggtgcaag cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagcgct
    45601 gcgtcgagaa tggtttcacc gctattagct tccagaacag cgccatcagg gcagagatcc
    45661 tgatgaggca aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag
    45721 cgcacgacga accgactggt ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac
    45781 gtttttaatc gcttgttcga tggcgtcaac atcatcgccc tgcgccactt cactcaggtg
    45841 agcggcagca tcgtcaatga cctgacgttc tgcggcgctt aacagcgcgg catcagcagc
    45901 cagcgcgccg tgcagacttt ccagcacacg cgccgcttct actttttgtt ctgccagcat
    45961 tcgggctttt acgtcctgct cggcatagct cattgagtct ttgatcatcg aagcgatttc
    46021 gctatcggtc agaccgtaag acggtttgac ctgaatagac gcctcaacgc cggtggattt
    46081 ctccatcgcc gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg
    46141 cgcaccgcca gccggtagcg ccgggatccc acgcagcgca aaacgcgcca gtgagcggca
    46201 gtcctgcacc agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc
    46261 tttaaaggtg gtgaaatcct gagcgcgggc caccggaata gtggtattac gcggaatcac
    46321 tttctccacc aggccgccca tcgtttcgag gcccagcgac agtgggatca catcaagcag
    46381 cagcatttcg ctgtctggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat
    46441 agcgacgact ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa attcgcctac
    46501 ccgttcacgc accagcggca cgcgagtaga accgccaacc atcaccactt ccagcacttc
    46561 atcagcttct acgcccgcgt ctttcagcgc acgacgacaa gccagtaagg ttcgttttac
    46621 cagtggcgcg atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac
    46681 gttaacggtc acggagtccg catcgctcag cgcgattttg gctgcaatgg cggcatccag
    46741 cagttcacgc tgaacgcggt tatcgctacg atcaggaatg cccgcctgct cgcgaatgta
    46801 atccgccagc agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc cggttgccag
    46861 cacttcaaac acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag
    46921 gtcataaaca gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc
    46981 ggtcggttcg ttaagtaagc gcagaacatg caaccccgcc agacgcgccg cgtctttggt
    47041 gccctgacgc tgggcatcgt caaagtacgc cggaacggtg ataactacac catccagctc
    47101 gcctgccagg gcttcagttg cccgcgccgc cagtgctttg aggatgtccg cagaaacgcg
    47161 caccgggttc agtagccccg ccgccgtttc aatcatcggc aggccgtttt cgctggcctg
    47221 gaattgataa ggcagatgcg gatagcgttg ctggatatca gccagcgagc gccccatcag
    47281 gcgtttaaca gaactaatgg tgttggcggt gtcaagcgct gcgttagtac gcgcgtcata
    47341 acccaccgaa tgcccttgct gttgatagtg aacaacagat ggcagcaggt ggcggccttc
    47401 atggtcggct aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg agttggttgt
    47461 gcccaggtca ataccagccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc
    47521 actaatttgt aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt
    47581 cgagttgttc ggcactgctc cgcagtttat cgagaaaacg cagcttacgc acggtatccg
    47641 ccgccgcgtc ccacgcctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa
    47701 acatcttttt cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga
    47761 tctcgtccag ctcttcgcgc agctccaact gttccatcag gaacgcggtg tcgcgcacag
    47821 tatgctgctc gctggcgaga tcaaaaccgt gcaaagaaag caaatattcc gcgcgcatta
    47881 acggatgacg cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga
    47941 gttgttccgc ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct
    48001 gaaaacgcag gctcagcgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaggg
    48061 tgaagtaatc cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa
    48121 aacaaacccc acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa
    48181 gctttcgccg caaccacact catctttgac gttcgggttg gtgaatttaa acccttcgtt
    48241 caggccttct tttacgaagt ccagttgcgt accgtccaga aattgcaggc ttttgccatc
    48301 gaccacgact ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac
    48361 aaattccagt acataagcca tacctgaaca tccggaggtt ctaacgccca gacgcaggcc
    48421 aaaccctttg ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag
    48481 tgtaatcgac ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg
    48541 caatggcggc tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca
    48601 gttcaagttc ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg agagacttcc
    48661 ctttcaccca ttcggtgacc agggagctgg aagcgatagc ggaaccgcag ccgtaagttt
    48721 taaaacgcgc gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcattacgt
    48781 cgccacaggc cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcgaagg
    48841 aacccacgtt acgcggattc tcgtaatgat cgataacttt ttcgctgtaa gccattataa
    48901 attctcctga ttccgatacc gattaatgat gagcccattc gatgctgttc agatccacgc
    48961 cctgcttgta catttcccac agcggagaaa ggtcacgcaa acgaccgatg gatttacgaa
    49021 ctaactcgat ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga
    49081 tagagctatg tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga
    49141 ggcttgctga cgtacaggcg gaacctgaag aaaccgcgag gtctttcagc gccataatca
    49201 gcgactcacc ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca
    49261 ggtcaccgtt caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca
    49321 ggccgcgcag acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcttcgc
    49381 ccatgccgac gatctggtga acaggcagag tgccggaacg cataccgcgt tcgtgaccgc
    49441 cgccgtgcat ttgcgcttcg atgcgtacgc gcggtttacg acgcacatac agcgcaccga
    49501 tacctttcgg gccatagatt ttgtgaccgg agaaagacat caggtcaact ttcaactggc
    49561 tcaggtcgat aggcagttta cccacgctct gggttgcatc aacgtgatag ataatgccac
    49621 gagcacggca catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattca
    49681 cgtgcatgat agaaacgagg atggtgtcgt cacgcatcgc tgcttcaagt tctttcaggt
    49741 cgataatgcc gttacgctgc ggtgccaggt aggtgacttc aaaaccttcg cgctccagct
    49801 gacggcaggt atccagtacc gctttgtgtt cggttttgct ggtgatgatg tgcttgcctt
    49861 ttttctgata aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag
    49921 aggtaaagac gatttcacgc ggatcagcgc cgaccagatc ggcaatctga ttacgggcga
    49981 tatctaccgc ttcttcagcc tgccagccga aacggtgaga acgagaggcc gggttaccaa
    50041 aggttccgtc catcgtcatg aactgcatca ttttctcggc aacacgcggg tccaccggcg
    50101 tggttgcgga gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca
    50161 ctcaatgcaa ggaatcaggc taccggctgg atgtacgacc gtgtttacgg agtatttagc
    50221 actccggcct gattctgaat tctttttatt aagcgcgtaa cttaacgtcg atcgcgtctt
    50281 gtgtgcgggt gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct
    50341 ggttattaac cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca
    50401 aatcacgcca cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct
    50461 gacaacgggt ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc
    50521 tgctggcatc tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac
    50581 catttttacg cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt
    50641 cggaaatatc agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg
    50701 cggtcacggc atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc
    50761 ccctgcccgg ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac
    50821 attcccgagt aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca
    50881 gtgccatttt tttatcgtgg cgtttgtacc tgttgtcgga tgcggtgtga acaccttatc
    50941 cgacctacgg ttctgtacat tgtaggcctg ataagacgcg ttaagcgtcg catcaggcaa
    51001 ccactgttat tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg
    51061 caggatattc aattcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt
    51121 catcacctgc cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag
    51181 atgaccataa aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac
    51241 ctgctcgcca ttttcctgag ttgccagcca ggccatgcgc acttcatagg caataacctg
    51301 aaccgccatc gccaggttca gcgagctgta ttccgggtta gccgcaatcg cgacatgata
    51361 atggcatttc tgcaactctt cgttggtcag gccgacacgt tcgcgaccaa aaaccagcgc
    51421 caccggggta tttgccgctt cagcaacgct tttcaaaccg cattcgcgcg ggtcgagcat
    51481 cggccacggc agcgtgcggg aacgtgcgct ggtgcccacc accaggctac aaccggctaa
    51541 cgcttcgtcg agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccccgccgc
    51601 cagggcaatc gcctgggagt caggtttcac cagtggatta accagccaca gattggttaa
    51661 tcccattgtt ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac
    51721 cagcacaatt cgaatatttt gcagcattgt ctttcttcgt ctaaagatta ttcgcgcatc
    51781 ttatcataaa acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt
    51841 ctttaacatc cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc
    51901 gcgcaaggcg ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag
    51961 ccagaaaggc agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag cggtgattat
    52021 cgacacgatt cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact
    52081 tgaaggtact gatcaggatg ttcaatgggt tattgatcca ctggatggca ctaccaactt
    52141 tatcaaacgt ctgccgcact tcgcggtatc tatcgctgtt cgtatcaaag gccgcaccga
    52201 agttgctgtg gtatacgatc ctatgcgtaa cgaattgttc accgccactc gcggtcaggg
    52261 cgcacagctg aacggctacc gtctgcgcgg cagcaccgct cgcgatctcg acggtactat
    52321 tctggcgacc ggcttcccgt tcaaagcaaa acagtatgcc actacctaca tcaacatcgt
    52381 cggcaaactg ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct
    52441 ggcttacgtc gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gcccgtggga
    52501 cttcgctgca ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg
    52561 tggtcataac tatatgctga ccggtaacat cgttgctggt aacccgcgcg ttgttaaagc
    52621 catgctggcg aacatgcgtg acgagttaag cgacgctctg aagcgttaat gactcaggcg
    52681 ggtgatatca ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact tcctcactgc
    52741 tttactttct ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc
    52801 gcaaggaaag gttatggcac tgccagtgaa caaacgcgtt ctcaaaattc tgtttattct
    52861 ctttgttgtt gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta
    52921 tcctgacgat aaactttacg gtcccgaccc ctggtcggcg gaatccgtcg aatttacggc
    52981 taaggacggt actcgtctgc aaggctggtt tattccttct tcgacgggcc ctgctgacaa
    53041 cgctatcgca accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc
    53101 gctggtcagt tggttacccg agcgtaattt caacgttttt atgtttgatt atcacgggtt
    53161 tggtaaatca aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagcgccat
    53221 caatgtggtg cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag
    53281 tattggcggg gcgaatattc ttgctgttat tggtcagggt gatcgtgaag gcatacgtgc
    53341 ggtgatcctc gactccacat ttgcctctta tgcaaccatc gccaaccaga tgatccccgg
    53401 cagtggctac ttacttgatg agagttacag tggcgaaaat tacatcgcca gcgtcagccc
    53461 gatcccgctt ttactcattc acggtaaagc cgatcacgtt atcccatggc agcacagcga
    53521 aaagttgtat agccttgcaa aaatgccaaa acggttgatc ctaatcccgg atggcgaaca
    53581 cattgatgct ttttccgatc gtcacggcga tgtttatcgc gaacagatgg tggactttat
    53641 ccttagcgcg ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa
    53701 aaccacctta agacatgcta atccactggt cagaacagtt taagatgaga aaaattctgt
    53761 gacgcttgcc aacatttctg atgattagca ttcccttcgc catttccctg agcaaacttt
    53821 agctattctt atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac
    53881 catctgtcct ttcggctccc cagcgccgct gccagatctt gctgacgctc tttctgccgg
    53941 ggttgaccgc caccacggca actttcagcg agcttaatgg tgtggctgat gatattgcca
    54001 gtcttgatat cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacga
    54061 ctggttatga cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat
    54121 ttcactggct acgacgtggt ttccgtctgt gtccgtcatt tattaccagc catttcaccc
    54181 ccgccctgaa gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca
    54241 atctacaagc gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaaacgcgcg
    54301 atattcattt cctgtgtctg tatctgcaat attgtttgct gcaacaccac gctggaatta
    54361 cgcctcagtt taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag
    54421 cgcaggaaat tggacgccac tggcagcgtc gggcgctcca gcctgtacca cctgatgagc
    54481 cactgtttat ggcactactt ttttccatgt tgcgggttcc cgatccattg cgggatgcgc
    54541 atcagcggga cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc
    54601 tgggaaatgt tcgtttttat gatgaacagg ggttatgtga tcagctttat acccacctcg
    54661 cccaggcgtt aaatcgcagt ttgtttgcca tcggtattga taataccctg ccggaagagt
    54721 tcgccagact gtatccacgc ctggtacgca ccacccgcgc ggcgctggcc ggatttgaaa
    54781 gtgaatacgg cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg
    54841 cctggctaat gcaggaaaac gatctgcatg aaaaacagat tattctactg accgggaatg
    54901 atagcgagcg agaagcgcag attgagcagc agttacgcga actaacgtta ctgccgctca
    54961 acattaagca tatgtcggta aaggcatttt tgcagacagg cgctccgcgt ggcgcggcac
    55021 tgattattgc gccttatacc atgccattac cgctcttttc accaccgctg atctatacgg
    55081 acctgacgct gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag
    55141 ggaacaactt tcgggcgcaa aaacattgcc ggcagcgcca ccagcgccat tacccagaac
    55201 acgccgtggc ccagatattg atacaggaaa cctgcgaaaa cggtcatgat agcgatactg
    55261 ccgcccatcg cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgct accctggcga
    55321 gcagcaatat aacgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt
    55381 tgcaccacta tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg
    55441 ccgcaaatcg ccgagatcaa cagcatatcg cgtgcactac aacggcggaa aagtttatta
    55501 ctcagcgcaa agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc
    55561 gaggccgagt agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt
    55621 gccccctgca ataaacaaac gcaggccaga aagcgccagt tctggcgaac cagcgccaac
    55681 cacgctgacc aaccggtgct ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga
    55741 cggatgagaa agccgagcag catggatgcc acgcccaacg tcaacagcgc gaggatcacc
    55801 cgataatcaa acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc
    55861 gagccccaca gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc
    55921 agtgcatcgg tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc
    55981 atcagccacg ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tgtcagcagt
    56041 gccagcacgc gcaaggcgga aatcaggcgg gaaggatcgc tgacgcgggg cgcgatgagc
    56101 aaactcccga gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc
    56161 gttaaaccaa tccctttaag ccagacgctc cagaaaggta gaaaaatgcc gtaactaaaa
    56221 aagtatgtga aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc
    56281 cgtcaggagg cgttagtctg gggttaatcg aatgttggtg caagttgaaa aataagaata
    56341 ttcgagcctg cttcccgatg tctgtaacgc cagttcacaa gcactaaaaa actgcgatcc
    56401 gcgacgcatg ttgcaaaaat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc
    56461 acaatggcga tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt
    56521 aaagcaatgg aaggcacatt tccggcaatg ggacgaaaaa caacctgccc ggtattaaaa
    56581 ttattcatat aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc
    56641 accagaatat tcgttgccac ctggacgata tttggctggc tgttttcttg cgcaaaccac
    56701 gctttaacga tcggcgcaag cgaaccggaa tacgccggat cggtactgac gaaattcacg
    56761 ccatccagtt gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac
    56821 accaccacta atggttcgtc aaaaagctcc agataatcaa tctccgggct ataaacagga
    56881 tggcgcatta agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg
    56941 attaaactca ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact
    57001 tttggcaata aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc
    57061 tgaacaattt tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg
    57121 tcctggagaa aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt
    57181 aacggaacac cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag
    57241 gtatgcagtt tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag
    57301 cgtaaatgcc gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag
    57361 aaattaacta tttcacatat tagcaaccaa ccagcaacat ccttatggca caaaaataga
    57421 aggtcaatac atcttatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca
    57481 tttaccaact gattgatacc caaaatggtc gggtcactcc gcgtatttat accgacccag
    57541 atatttacca actggagctt gaacgtattt tcggccgttg ctggttattt ctcgcccacg
    57601 aaagccagat cccaaaacct ggtgatttct ttaacaccta catgggggaa gatgcggttg
    57661 tcgtagtgcg tcagaaagac ggcagcatta aggcgtttct caaccaatgc cgccaccggg
    57721 ccatgcgtgt gagttatgca gattgcggca acactcgcgc ctttacctgt ccgtatcacg
    57781 gctggtctta tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc
    57841 cacaagggtt gtgtaaatcc cactggggac taaacgaagt tccttgtgtg gagagttata
    57901 aagggctgat ttttggcaac tgggatacca gcgcaccggg cctgcgtgat tacctgggtg
    57961 acattgcctg gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg
    58021 gcggcgtaca aaagtgggtg atcaactgta actggaaatt cccggcagag cagttcgcca
    58081 gtgaccagta tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag
    58141 atgatggcag cgataagcgc ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg
    58201 ccaaagatgc gctgcaattt ggtcaggacg gtcacggtag cggtttcttc tttactgaaa
    58261 aaccggatgc taatgtctgg gtcgatggcg cagtttcaag ctattaccgc gaaacctatg
    58321 ccgaagcaga acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata
    58381 tattccccac gctttcatgg ctaaacggca ctgccacact ccgcgtctgg catccgcgcg
    58441 gccctgatca agttgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag
    58501 ttaaagccgc ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc
    58561 aggatgactc ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca
    58621 acagcaaact gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc
    58681 ctggcattac taactatatc ttttcagaaa ctgccgctcg tggaatgtac caacgctggg
    58741 ccgatcttct gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc
    58801 aggaggtgat gaaatgagtg cgcaagtttc actagagtta catcaccgca ttagccagtt
    58861 tctctttcac gaagccagct tactggacga ctggaaattt cgtgactggc tggcgcagct
    58921 cgacgaagag attcgttaca ccatgcgcac cacagttaac gcgcaaacac gcgaccgccg
    58981 caaaggcgtc cagccaccga caacctggat ttttaatgac accaaagacc agctggagcg
    59041 gcgaatcgcc cgtctggaaa cgggcatggc ctgggcagaa gagccgccgt cacgcacccg
    59101 tcacttaatc agcaactgcc agataagcga aaccgacatc ccaaacgtat ttgctgtgcg
    59161 ggtaaattat ctgctttatc gggcacaaaa agagcgcgat gaaacattct atgttggaac
    59221 gcgtttcgac aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat
    59281 cgtcctggat caagcggtaa tcacttccca taacctgagt gtactgttct gatgaatcga
    59341 atttatgcgt gtcccgttgc ggatgtgccg gagggtgagg ctctccggat cgatacctcg
    59401 cccgtcatcg ccctgttcaa cgttggcggc gagttttatg ccattaacga tcgttgcagc
    59461 catggtaatg cgtcgatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg
    59521 ctacacgccg ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat
    59581 ccgctcacca cttatccagt acacgttgaa ggtggtgaca ttttcatcga cttaccggag
    59641 gcgcagccat gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat
    59701 tagggctagc gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac
    59761 tgtcggcggc aaaagtcgcc agtctgcgtc agcgatttgg tgaacatatt ctggcggtgg
    59821 aaggtaacgt gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgactcgtt
    59881 ccggcaagct ggattgtttt atcggcaatg caggcatctg ggatcacaat gcctcactgg
    59941 ttaatactcc cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaatgttc
    60001 tcggttacct gctgggcgca aaagcctgcg ctccggcgtt aatcgccagt gaaggcagca
    60061 tgattttcac actgtcaaat gccgcctggt atcctggcgg cggtggcccg ctgtacaccg
    60121 ccagtaaaca tgccgcaacc ggacttattc gccaactggc ttatgaactg gcaccgaaag
    60181 tgcgggtgaa tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc
    60241 tcgggcaaag tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt
    60301 taccgctgca atttttcccg caaccggctg attttacggg tccgtatgtg atgttggcat
    60361 cgcggcgcaa taatcgcgca ttaagcggtg taatgatcaa cgctgatgcg ggtttagcga
    60421 ttcgcggcat tcgccacgta gcggctgggc tggatcttta aggaagcacg atgaaagaaa
    60481 aaacgatcat tattgtcggt ggcgggcaag cggcggcaat ggctgcggcc tcgctacgcc
    60541 agcaagggtt caccggtgag ctgcatctgt ttttcgatga gcaacatctt ccttatgaac
    60601 gccccccgct ctcgaaatcc atgttgctgg aagattcccc gcagttgcag tctgtgttac
    60661 ccgctcactg gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacat
    60721 tgggccgcga cacacgagag ttagtgttag ccaacggcga aagctggcat tgggatcagc
    60781 tttttatagc aaccggcgcg gcagctcgac cgctgccgtt gcttgataca ctgggagaac
    60841 gctgctttac cctgcgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg
    60901 aacggtcagt cgtgattatc ggtgccggaa ctattggtct ggaactggct gccagcgcca
    60961 cgcagcgcag atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg
    61021 caccaccgcc cgtgcaacgc tatcttttac agcgccatca gcaggctggt gtgcgcattc
    61081 tgctcaataa tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa
    61141 gtggcgaaac gcttcaggcc gatgtggtga tttacggtat tggtatcagc gccaacgacc
    61201 aactggctcg cgaagccaac cttgatacgg taaatggtat tgtcattgac gaggcttgcc
    61261 gcacctgcga tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg
    61321 gtgcactaca ccgctgcgaa agctgggaaa acgccaataa ccaggcgcaa attgccgctg
    61381 ccgcaatgtt agggctaccg cttccgcgac tgccgccgcc gtggttctgg agcgatcagt
    61441 acagtgataa cttacagttt attggcgata tgcgtggcga tgactggctt tgtcgtggta
    61501 acccggaaac tcagaaggcg atttggttta atctgcaaaa cggcgtgctt atcggtgcgg
    61561 taacgctgaa tcaggggcgt gagattcgcc caatccgcaa atggatccag agcggcaaaa
    61621 cgtttgatgc gaaactgctg atagatgaga acatcgcgct taaatcactg taaccaggat
    61681 aattagcgaa tatctcaatg cctggggcgt ggcgaggtgc aagagtgtgt attacgttta
    61741 aatcacatta tcttgcaaag ggattggtta tgaacacact acgttatttt gattttggag
    61801 ctgcccgccc cgttttgtta ttaattgccc gtatcgccgt ggtcttaatt ttcattattt
    61861 ttggttttcc caaaatgatg ggctttgacg gtacggtcca atatatggcc tcgttgggcg
    61921 cgccgatgcc gatgctggca gcgattattg cggtagttat ggaagtgccc gccgcgatat
    61981 taatcgtgct tggctttttc acccgtccgc tggcggtgct gtttattttc tacacgctgg
    62041 gtacggcggt gattggtcac cattactggg atatgaccgg cgatgcggtt gggccaaata
    62101 tgattaattt ctggaagaat gtcagtatcg ctggcgcatt cctgctgttg gcaattaccg
    62161 gaccgggggc gatttctctc gatcggcgtt aggaaagatg ccggatgcgg catgaacgcc
    62221 ttatccggca ttttataaat tacagcaacg caacccgcaa cgacatctct gaagttgttg
    62281 attcacctgg tgcaagcgca agaagatcac caccttccgg gcgatgatgg tcatctggcg
    62341 catggctcat cggttccaga cagaagaaat caaacgcata tcctttatca aacgccgggt
    62401 cggaaacaaa gatgaaataa cacggtgcag gtggcgtcgt ttccatgatg atggcataac
    62461 cttcctgcgg ctgctcgatg cgggcctgac cattccatcc ggcaaaacca ttgttcaccc
    62521 actggcgcgg caacggcgcg agctggttaa aatccagttc ctgcgatagc tgctcgcaaa
    62581 actcacccgc cagccactgc tcccgttcca gccagtaacc gctcgcctgc gcctgaatcc
    62641 gcgtttgtgg tgacaacgga aaataaggat gccagccggt accaaatggc agcgtctctg
    62701 ccccttgatt ggtgacagag agcgtcaccg tcagcgtatc cgccgttaaa tgaaacgcct
    62761 gacttacccg atagtgatag acaccgctgc gatgttcata caccagacac aaactatcat
    62821 cgctgtgcga gacacattgc cactcgccca gccagccatc gccgtgtaga tagtgcgcat
    62881 cccactcaac attcggttgc agttgatact cacgcccctg ccagacaaaa cggttgccgc
    62941 tcacccggtt tgcaaatggc accagcggaa aacaggaggc atcggttgcc acaccgcttt
    63001 ttttaccagg acgtaagagc ggcgtcgtat cgcgccagaa gccttcgatt acgccgccct
    63061 ggtcagaaac gtccagcttt agcgccccgt gggataaggt atagatggtc atccgctaac
    63121 tccttaatcc gagaagttaa tcacaacttt cccgcatttg ccgctcgcca tcagcgcata
    63181 ggcatctcca gcctgttcca gcgtgaagcg atgggtaatg gcgttacgcg gccacagttt
    63241 ccagtccgtc agatcatggg cgcatttttc catatggaac agactggtca cccaggagcc
    63301 gataatccgc cgttgatggt gcatcagatc ggcgctgacc tcgaattcca cttttccggt
    63361 ttcaccaatg taaaccaccc gtccccagtc agcggtggat tgcagtgcca gcaagcgacc
    63421 tgcggcatta ccagaacaat cgagcgcaac atccgcgcca ccgtgggtga gttcggcgat
    63481 aatctgcggc agaccttcgg tggttgctaa atagccgtga tccatcaccc ctaactgttt
    63541 tgccatcgcc agacgttccg gcagcatatc aacgccgatg atccgttttg caccgcgacc
    63601 tttcgccagc atcatcgcca tcatgccgac cggccccaga ccgaccacca gcacgttatc
    63661 gctgccggaa acttcgccgc gcaaaatccc ttcataggcc gtgccaacgc cgcaactgat
    63721 aaacgcacca tcttcgtagc tcagcgcatc cggcaggagg atcagatctt tttcttccgc
    63781 cagcagatat tcggcatgac cgccgtcacg ctgccagccg taagccgctt ttcctttgcc
    63841 agtacaagag ataggaaagc cgcggcggca gttcgggcaa aaaccacagc cagaaatgtg
    63901 atacaccagc acgcggtcgc cctctttaaa atggcggcag ccttgcccca tcgccacaat
    63961 ctgcccgcac ggttcatgac cgttgataaa gccctggtat aacggtttat cgggtgccgc
    64021 cgctgtggca cggtgttgat gatagatata gtggacatcg cttccgcaaa tcccggagga
    64081 tttcattttg atcagtacct ggttaatccc cggcgtcggc accgcaactt cccgcagatc
    64141 gacggtcgaa tttcctggta aataagctgc cagcatcgtt ttcataaatc cctcattaac
    64201 aatacgatta attttcatcc ctgccgcacc cgcgccaggg gcgttaaggt ttagcgtttc
    64261 gctttactgc ttcgctgggt cagcaagata ttcgccagca ccgccaccac gatgatgacg
    64321 ccgcgtacca cctgctggaa aaaggagtta ataccgagca gcaccagacc gttaccgatt
    64381 agcgtaatca ccagcacacc aagcaatgta ccgaacaggg aaccgcgacc gccggaaagt
    64441 gccgtaccgc cgaccacgac cgcggcgatg acgtcaaact ccagaccgtt tgcggcacct
    64501 gcgttaccgg aaccgaggcg cgccgccaac aaaatgccgg tcaccgccgc taataatccc
    64561 gaaagggtaa agataagaat gcgtacccga cgaacgttga tgccgcacaa ctgcgccgcc
    64621 gtggcattac cgccaacggc aaaaaccgag cgcccgaagg cggttttgcg gctgatgaac
    64681 acaaacagcg caaacaacac catcatgatc agcgcagata ccggcacacc gagaaattgt
    64741 ccgcccagcc agtccagcac ctcattttcg tcaatcggca ctggcagggc gttggtcata
    64801 aacagcccca ttccgcgcag ggcgctccac agccccagcg tggcaacgaa acttggtacg
    64861 ttaaacacgc cgcgcagcac cccggcgagc gttcccatca gcgcgcctaa cagcaacacc
    64921 agcagacacg ccaccgccag cggaacttca aattgcagca aaaatgccag gcacaccgag
    64981 acaaaagcca ccatcggccc aacgctgaca tcaatttcac cggagataat aatcagcgtc
    65041 atcgcccagg cggcaatccc aatggtggcg gcatcgcgca gcacgttcat ctggttattc
    65101 aatgagataa agccaggcga gttcagggag aagaccagat aaagaatggc aatcaccacc
    65161 agcaaaccga tctcattaat atggcgactg acaaattgtt tgagcgagac gctcttgccc
    65221 tgcggcaatg gtaatgacga agcagacatc ttaggttcct cggtaaaatt catcagtgca
    65281 cagacagaat ggcggacatc agctcatcca cattgaccgg agcgtgaaac tcctgcgaga
    65341 acgtgccgtg ctgtaataac aggatgcggt cacacaccaa cggcaactcc tccacttcac
    65401 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    65461 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    65521 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccgccagaa agcgtgccga
    65581 tgggtgtttc gctactggcg gccttgaccg tcatccgctg catcacctct tcggtcaggc
    65641 ggcggatggt ggaccattgc agcacaccgt tggcgctgat tttttgccga ttggtcagca
    65701 ctgtattttc gtcaacgccc aaccagggaa tgatccccgc ttctttgcgg ttttctggcg
    65761 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcgtgatt ttctcgccgt
    65821 tgataacaat ttcgccctgt tcatactcct ccagcccaac aatcgccttc agcaattcac
    65881 tgcgccctgc ccccagcaga ccagcaatgc cgagcacttc gccacgacgt agcgtaaaac
    65941 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagcacg gcctgatcca
    66001 caatttcctg aggggctacc ggcgcaatat caacgtgatc gcgcccgagc atcagcgaca
    66061 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    66121 taacggtggc acaggaggca atgcggcgaa tttcttccat ccggtggctg acataaatca
    66181 ccgccacgcc cagtgccgac atctttttca ccgcgctgat caccagttca acttccgcac
    66241 tcgcaagcga actggtaggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    66301 gcgcaatttc caccagctgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    66361 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    66421 caatcatgcc gttgcggcgg ggccactgac tgaggcagag gttttccgcc actgtcagcc
    66481 cttccaccag acttaattcc tgataaaccg cgcgcacccc cagttcagcg gcacggcgag
    66541 tcagcgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    66601 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    66661 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    66721 cgggataacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    66781 acatagggac ctctgcaaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    66841 tgtttaccgt cttccgtttt ggtatagaga tcgataggca cctgaatcac tttttcaccg
    66901 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    66961 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    67021 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    67081 tttaccgcgc cgagtgtcgc accgcccgat tcccccataa tggcgttgag atccggcgtg
    67141 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctgatta
    67201 gcgacaattt gcgcgccggg aacgcgggct tttaatactt cttcaaaccc tttacgtcgt
    67261 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaattttg
    67321 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagcgggtca
    67381 cccaccagat atgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    67441 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    67501 gacactgccg ataaaataat agcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    67561 ctttctttcg aaatattacc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    67621 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg agtatcaagg
    67681 tatattgcgc caatggtcat ttctttatcc gcagccctgg caaataaagc gctacctaat
    67741 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agagcctcct
    67801 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    67861 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    67921 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    67981 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    68041 ttttctttat gctgctgttg taaatcgcca tacagcgaca acagatcagg ttgtgaaaca
    68101 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    68161 ctaaacagtt gctgtgcggt gtgttgttcg cccagcagtc gcagcgccat tccttgccag
    68221 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    68281 gtcgccgcca gacgtaaaca acgcgtcgct tcagtttcat cgccctgcgc gttggcgcat
    68341 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    68401 ttctccggat aatgcagcgc ggcatgaagc agttcgcggg cctgctgcgg ctgtctggca
    68461 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    68521 tccccgcctt cccacgggtg gaatttgcgc gtggcgagaa tgtccgccgc tttgtctgcc
    68581 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    68641 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggatcc acttaactta
    68701 tcaagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    68761 tcaagataac gcgcggccag ctcataatcg tgttgcttat tccacgcatg gatcgctaac
    68821 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca aaaggcaatg
    68881 gctttgttgt agctacgttt gttgtagtag aagcaagcca gtaaatggcg agcaaaccag
    68941 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    69001 tgcgggaaga catcaatggc ttttgcgacc ggttcgccac gttcggcttt cggcaataag
    69061 ctcgcctgca gataaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    69121 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagggc gttaacgtcg
    69181 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    69241 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    69301 cgcgcgttgt cctgacgacc acttaacacc tgcagcaggt tatgcaggca aagcacttcc
    69361 tgattggttg ggcaggcgcg aagactttgt tggcaaaaat ccaggcccgc gtcgaagtga
    69421 ccattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttgct gttgccgctc
    69481 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    69541 ctggcgcgaa tcaaactcgc ctgtccgcac tgcggatttt tgttcagcgc atgtgcgcgt
    69601 ttcagcgcct gagtggcata cgccaccgct tgtgggaaat ctgcgcggtt atattccagc
    69661 atcgccagcg ccaggttaca gcggtaatcc agcggatcca gcgccacacc gcgcaggtag
    69721 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    69781 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    69841 ggcaactctt gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    69901 agctcaatgg tcagcctttc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    69961 ggtgtcaagg ccacggcatc atcaagtaac gcgttgcatt tgccgatttc gcggatcgcc
    70021 aggcggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    70081 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    70141 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    70201 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcagg
    70261 ctcttgtccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    70321 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgcca
    70381 acaaaatcgt actgtgattt ttcagccata tatgaggttg gaacgggcac atttttatag
    70441 cgagaaatgt ccactccagc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    70501 aaagcggaga cggcccgttt gccgtgatca aacaccgccg ttacatccgg cgggaaaacg
    70561 ctctgatgcc cttccccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    70621 gtggcgttgc cgttatagac gcggctggcg atttccagcg ccgcccggtc agggcgcagg
    70681 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    70741 tgtgcgccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    70801 tggtgttgcg gccagttaaa ctcaatccca ccggagatcc acggtcccag cagccccacc
    70861 agcgcaggtt taatgacttc attgtgataa acaaaatcgc gttgtttcac tttatcccat
    70921 gcgcgatgca cacgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    70981 agccacaccg cctgccagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgcca
    71041 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    71101 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    71161 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    71221 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    71281 tgaagatgag agcctgcatt aacaatcaac agatccgcca ccataacaaa tgcgtgattc
    71341 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccga ctggcgcaaa
    71401 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    71461 atatcgacga tgaaagccat acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    71521 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    71581 atgcttgttt aagtccgaaa ggtgaatttg agtatttaca gattgatgca ccgacgccgc
    71641 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    71701 ataccatcaa cctggcgttg gcaatccacg gtcaggttga tcctgtgacc ggcgtgtcgc
    71761 aaaccatgcc gcaagcgccg tggacaacgc cggtggaggt gaaatatctg ctggaagaga
    71821 agctcggcat tcgggtgatg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    71881 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    71941 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    72001 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttat ggctgcctgg
    72061 aaaccgtcgc ctcgttaagc gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    72121 cggttagtac tcaacttgat cctgaaaaac tgactacagc gcagttaatc gctgcctggc
    72181 aaagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    72241 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    72301 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    72361 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg ctttgggcaa ttaagccgcg
    72421 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    72481 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    72541 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    72601 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    72661 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    72721 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    72781 cagccagttc tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta
    72841 cacgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    72901 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa
    72961 ccagatcaac caggaacagg tggttatcag tgccgccgga aaccactttg tagccgcgtt
    73021 ccaggaacac ttctaccatc gctttagcgt ttttagcgac ctgctgctgg taagttttga
    73081 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    73141 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    73201 cacctttcgc caggatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    73261 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    73321 cgtgcgccat atcaacgaac aggtaagcac cgatgctgtc agcgatttca cgcattttcg
    73381 cccagtccac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    73441 ctttggcttg tttttccaga tcggcgtagt cgatatgacc ggtagcatcg ataccgtaag
    73501 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    73561 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    73621 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    73681 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    73741 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    73801 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    73861 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    73921 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    73981 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    74041 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    74101 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    74161 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    74221 agatacatca attaagatgc aaaaaaagga agaccatatg cttgacgctc aaaccatcgc
    74281 tacagtaaaa gccaccatcc ctttactggt ggaaacgggg ccaaagttaa ccgcccattt
    74341 ctacgaccgt atgtttactc ataacccaga actcaaagaa atttttaaca tgagtaacca
    74401 gcgtaatggc gatcaacgtg aagccctgtt taacgctatt gccgcctacg ccagtaatat
    74461 tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc gcgcagaagc acaccagctt
    74521 ccagatcaaa ccggaacagt acaacatcgt cggtgaacac ctgttggcaa cgctggacga
    74581 aatgttcagc ccggggcagg aagtgctgga cgcgtggggt aaagcctatg gtgtactggc
    74641 taatgtattt atcaatcgcg aggcggaaat ctataacgaa aacgccagca aagccggtgg
    74701 ttgggaaggt actcgcgatt tccgcattgt ggctaaaaca ccgcgcagcg cgcttatcac
    74761 cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca gaataccgtc cggggcaata
    74821 tctcggcgtc tggctgaagc cggaaggttt cccgcatcag gaaattcgtc agtactcttt
    74881 gactcgcaaa ccggatggca aaggctatcg tattgcggtg aaacgcgaag agggtgggca
    74941 ggtatccaac tggttgcaca atcacgccaa tgttggcgat gtcgtgaaac tggtcgctcc
    75001 ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca gtgacgttaa tctctgccgg
    75061 tgttggtcaa acgccaatgc tggcaatgct cgacacgctg gcaaaagcag gccacacagc
    75121 acaagtgaac tggttccatg cggcagaaaa tggcgatgtt cacgcctttg ccgatgaagt
    75181 taaggaactg gggcagtcac tgccgcgctt taccgcgcac acctggtatc gtcagccgag
    75241 cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt ctgatggatt tgagcaaact
    75301 ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc tgcggcccgg ttggcttcat
    75361 gcagtttacc gcgaaacagt tagtggatct gggcgtgaag caggaaaaca ttcattacga
    75421 atgctttggc ccgcataagg tgctgtaatt tgatgttgcc ggatggaaac atccggcaac
    75481 ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg gtacggatgc gaatgacccg
    75541 tgccacgtca aagacgaaga ttttaccgtc accgattttg ccggtttgcg ccgtgcgaat
    75601 aatggtatcg acacaggtat cgacaatgtc gtccggtacg acaatctcaa ttttcacttt
    75661 cggcagaaaa tccaccatat actccgcgcc gcggtacagc tcggtatggc ctttctggcg
    75721 accaaagcct ttcacttcgg tcaccgtcat gccggtaata ccgacttcgg ccagtgcttc
    75781 gcggacatcg tccagcttga agggttttat aatcgcatca atctttttca tgctattcct
    75841 tgaaaaggtc gcctgtcttt tgatctgcta aacgtaacac ataacgccaa ttcattcctt
    75901 gaaatcgttt gcatccagct cgtgtcggga aagcagttta taaaattctg tccggttgcg
    75961 ccccgccatt ctcgccgcgt gggtgacgtt gcctttggtg atttgcagca gcttacgcaa
    76021 atagttgagt tcaaactgat tacgtgcctc aacaaaggtt ggcagcgccg tattttcacc
    76081 ctccagcgcc tgctccacca gcgcatcact aatcaccgga gatgaggtca gcgccacgca
    76141 ctgttcaatc acgttgacca actggcgcac attacccggc cagctcgcgg tcatcaggcg
    76201 tttcatcgca tcggtagaga acgcgcggac aaacggttta tgtcgctctg ccgcctggcg
    76261 caacaggtga tttgccagta gcggaatgtc ttctgtgcgc tccgccagtg ccggaatttt
    76321 caggctgaca acgttgaggc ggtaatagag atcttcgcgg aattccccgc gcgccatcgc
    76381 ttttggcaga tcacggtgag tggcagaaat aatccgcaca ttgatatcaa tatcgcggtt
    76441 actgcccagc gggcgcactt tacgctcctg caacacgcgc agcagtttga cctgtaacgg
    76501 cgcaggcata tcgccaattt catcgagaaa tagcgttccg ccttccgccg cctggaataa
    76561 accttcgcga ttgctgacag cgccagtaaa cgcgccacgc gcatgaccaa acagctccga
    76621 ctccagcaat tgctcgggca atgcgccaca gttaatagca ataaatggtt tgctgttgcg
    76681 cgggctggcg ttgtggatag cctgggcgaa aatctctttc ccggtgccgc tctgaccgtt
    76741 aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc tgttccagca aacgcagcat
    76801 cagctggctg cgggtgacaa ttgcctcgcg ccagcgttca tcggtggctg gcgcggattg
    76861 ctccagcgca tcgtcaattg cctgatatag cgcgtctttg tcgacaggct tggtgaggaa
    76921 actaaaaacg ccctgctgtg ttgcagcaac ggcatcgggg atagaaccat gcgcagtaag
    76981 aataattact ggcattcccg gctgcacttt ctggattaca gcaaatagct gcataccgtc
    77041 catttcatcc atccgcaggt cgctgataac taaatctact ttttcgcgat tcagtacccg
    77101 taatccttca gcgccacttt ccgccgtgac cacactgtag ccttcgctgg tcaggcgcag
    77161 gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat aataaatgcg caggtttatg
    77221 gctcatcagg agtgacctca tgggtggatg gcgcgggctt ttcactctcg tgcggcgtat
    77281 ccggcgagaa atttccagcc ggtttgcggg tcgagagctg gcgttcaata tcggtcaggt
    77341 tttccagctt gcgggtggtg agttccagtt gctgctgtag aacgtgatgt tgctggcgca
    77401 atgtatccag ctcgctgtcg ctggactgct ggagtttact gtaacgttgg cgctcttccg
    77461 ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg atacagtggg cgaacctgtg
    77521 ccgggatctc ggtacttaac gcttcaatac gcgcgaccag ctggcggcgc tcatatggcg
    77581 taattttggc gtcggcgagc aaaatccctt gtttaaaggt attttgccag ctgccgtcgt
    77641 catattggcg agcttgctga cgcgactgcg caggcattaa acgatcagca caatccatcg
    77701 cccgcagcca gtaaagcgga ttggtttcgg ttgatttacc ttgcagcgcc cagatgtcgc
    77761 tacattcagt agaaagatag tcagccagtt gataaaccgg aattttttct tctgctggcg
    77821 tatcaatggc tggcttattg tgattctgca cgcaacccag caatgccaga catggcagcc
    77881 ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat gtgtcgcata ttcaccagac
    77941 ttaaagccta tcccagtggg cgtaattgtt gcagacagtc tggacatgga cagcgcggag
    78001 aaaccggagc gtacatatcg tacgtgaaga tttcgagcac tgcccggggc cgaactgaca
    78061 aataaaatag cctgatagga taggctgtta gcattatttc gtgtttttcg acgacggtaa
    78121 ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc agatacagtt ccccttgcat
    78181 acggcgaata caatccctgg caatgcttaa tcccagaccg ctgcccttca ccgccccttt
    78241 tcgctggtgg cttccctgaa aaaagggttc gaagatcatg gcgcgttcct cttgcggaat
    78301 gggcgtgcct gtattgatga catcaatata aacccgcgca ccatgtaaac tgctgcgaag
    78361 gcaaatgtta ccggattcag ccccgtagtg caccgcattg gagtaaagat tatccagtac
    78421 gctcatcagc agcattggct ccgccaggca agctgttgct ttgagatcga cgtcggtatg
    78481 catcatttta gcccgtgcgg gcaggctatg agcagaaacc actgtctcca ccagcggtgc
    78541 taattcaaca ttctccagtt ccaccgcact gtccgcctgt ttacggttgt aatcaagcag
    78601 ttgttcgatc agtttttgca aattgcggct gctgctatca agaatgctca ccacctcttt
    78661 ttgctctggc gtaagcggcc cgacaacctg atcagccagt aattcagtgc cctcgcgcat
    78721 actcgccagt ggcgttttta attcatgaga taaatgtctt aaaaattgat ggcgttggga
    78781 ttccagccat gacaggcgct cacttaacca aagaatacgt tgcccaaccg agcgtaactc
    78841 gctcggtcca ctgaacgaaa cgctattgcc cagagaacgc ccttccccca gacggttgat
    78901 catgcgctcg atatttttca ccggcccgat aatcatccgc gtgaaaagta gcaccatcac
    78961 cagactcacc agaaatagca ccagcgattg ccaaccaaaa tattgcccac gttcggcgat
    79021 ttcacgctga agttgctgcc cacgagagaa caccactgtg cgcgtggcct gcaccatttc
    79081 ggtattggca ctggcaaagg cttcaagacg cgcggcggcg gcagcatcgg gaccactgtt
    79141 gttacactga agttgggcca gattgttcaa atcctgacgc aatgcctggt aaagtttatc
    79201 gtctggcagt acgcctgcgt gggcatcgag catttcgctg taacgcttgc gctggctttg
    79261 ataaaccttc gcaagcgtag ggtcgtccag cacgcaatac tgacggtagc tacgctccat
    79321 ctccagcgcc gcgttggtca tcgcttcact acgccgggca tcaataagcg tagtgcggtt
    79381 aaccagcgcc gcctgatcgc taagcgcatt caggctttgc caggcttgcc atgccagcac
    79441 caacaggggt agtagaatta gcaaaaatgc cagcattacc agttgtcgta atgagcgggg
    79501 aaaaacgggc cagcgtttca aggtgttact ctcgtcagac gcgaatagcc tgatgctaac
    79561 cgaggggaag ttcagataca acaaagccgg gaattacccg gctttgttat ggaataaggc
    79621 ggtgcctaac tcgacgtttc gcccgatggt tgatatagct acgctgatat cagaagttgg
    79681 acggcaggca ccttgttgtg cgtcattcga attttatgta gcacgtcccg aaggggctga
    79741 cataagtcgg tgaatgagcc actggttact attatgcagt aactgtgcca ataaagaaaa
    79801 tagtttggta acgtactgat tatacgttgc tttgaggggt ttatgtcttc tccgctgttt
    79861 gaatgtaaat cagccactgt gtcgctaaaa agagacaact taagataaac ttattagata
    79921 atattaaaat caatgagtta agtgtcgcca gaaagcgaca cggcaaacca c
//
LOCUS       DAOFQD010000005        79926 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.005, whole genome shotgun sequence.
ACCESSION   DAOFQD010000005 DAOFQD010000000
VERSION     DAOFQD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.005"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            9..593
                     /locus_tag="QBF46_000313"
     CDS             9..593
                     /locus_tag="QBF46_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6672553.1"
                     /translation="MNKGTKIAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPMTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGIAATGVGIRIDDAESGNIMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYCVSTVEASEITPGEADAVVNVTLDYR"
     gene            673..1368
                     /locus_tag="QBF46_000314"
     CDS             673..1368
                     /locus_tag="QBF46_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDQ6672554.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKIDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1398..3914
                     /locus_tag="QBF46_000315"
     CDS             1398..3914
                     /locus_tag="QBF46_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6672555.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGVGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3925..5016
                     /locus_tag="QBF46_000316"
     CDS             3925..5016
                     /locus_tag="QBF46_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6672556.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKNNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5059..5919)
                     /gene="rsmI"
                     /locus_tag="QBF46_000317"
     CDS             complement(5059..5919)
                     /gene="rsmI"
                     /locus_tag="QBF46_000317"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDQ6672557.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5984..8020
                     /gene="lpoA"
                     /locus_tag="QBF46_000318"
     CDS             5984..8020
                     /gene="lpoA"
                     /locus_tag="QBF46_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDQ6672558.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDAQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITLRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRNLSWLQYQQGQVVPVS"
     gene            7978..8373
                     /locus_tag="QBF46_000319"
     CDS             7978..8373
                     /locus_tag="QBF46_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDQ6672559.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIALIMREGRTTIFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8393..8983
                     /gene="diaA"
                     /locus_tag="QBF46_000320"
     CDS             8393..8983
                     /gene="diaA"
                     /locus_tag="QBF46_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDQ6672560.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8993..9568
                     /gene="yraP"
                     /locus_tag="QBF46_000321"
     CDS             8993..9568
                     /gene="yraP"
                     /locus_tag="QBF46_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDQ6672561.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9682..10722)
                     /locus_tag="QBF46_000322"
     CDS             complement(9682..10722)
                     /locus_tag="QBF46_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDQ6672562.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10795..11430)
                     /locus_tag="QBF46_000323"
     CDS             complement(10795..11430)
                     /locus_tag="QBF46_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDQ6672563.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRNKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11558..12076
                     /gene="yhbO"
                     /locus_tag="QBF46_000324"
     CDS             11558..12076
                     /gene="yhbO"
                     /locus_tag="QBF46_000324"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDQ6672564.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12056..12499)
                     /locus_tag="QBF46_000325"
     CDS             complement(12056..12499)
                     /locus_tag="QBF46_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDQ6672565.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12550..12852
                     /gene="yhbQ"
                     /locus_tag="QBF46_000326"
     CDS             12550..12852
                     /gene="yhbQ"
                     /locus_tag="QBF46_000326"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDQ6672566.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12839..13342)
                     /locus_tag="QBF46_000327"
     CDS             complement(12839..13342)
                     /locus_tag="QBF46_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ6672567.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13336..13860)
                     /locus_tag="QBF46_000328"
     CDS             complement(13336..13860)
                     /locus_tag="QBF46_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDQ6672568.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14069..15064
                     /locus_tag="QBF46_000329"
     CDS             14069..15064
                     /locus_tag="QBF46_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDQ6672569.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15073..15951
                     /locus_tag="QBF46_000330"
     CDS             15073..15951
                     /locus_tag="QBF46_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDQ6672570.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16032..17039
                     /locus_tag="QBF46_000331"
     CDS             16032..17039
                     /locus_tag="QBF46_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HDQ6672571.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17157..18401)
                     /gene="mtr"
                     /locus_tag="QBF46_000332"
     CDS             complement(17157..18401)
                     /gene="mtr"
                     /locus_tag="QBF46_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDQ6672572.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18555..20444)
                     /gene="deaD"
                     /locus_tag="QBF46_000333"
     CDS             complement(18555..20444)
                     /gene="deaD"
                     /locus_tag="QBF46_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDQ6672573.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20437..20517)
                     /gene="yrbN"
                     /locus_tag="QBF46_000334"
     CDS             complement(20437..20517)
                     /gene="yrbN"
                     /locus_tag="QBF46_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDQ6672574.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20624..21508)
                     /gene="nlpI"
                     /locus_tag="QBF46_000335"
     CDS             complement(20624..21508)
                     /gene="nlpI"
                     /locus_tag="QBF46_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDQ6672575.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21617..23752)
                     /gene="pnp"
                     /locus_tag="QBF46_000336"
     CDS             complement(21617..23752)
                     /gene="pnp"
                     /locus_tag="QBF46_000336"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDQ6672576.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAQLLSEDQMLCAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(23999..24268)
                     /gene="rpsO"
                     /locus_tag="QBF46_000337"
     CDS             complement(23999..24268)
                     /gene="rpsO"
                     /locus_tag="QBF46_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDQ6672577.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24417..25361)
                     /gene="truB"
                     /locus_tag="QBF46_000338"
     CDS             complement(24417..25361)
                     /gene="truB"
                     /locus_tag="QBF46_000338"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDQ6672578.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25361..25762)
                     /gene="rbfA"
                     /locus_tag="QBF46_000339"
     CDS             complement(25361..25762)
                     /gene="rbfA"
                     /locus_tag="QBF46_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDQ6672579.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25926..28598)
                     /gene="infB"
                     /locus_tag="QBF46_000340"
     CDS             complement(25926..28598)
                     /gene="infB"
                     /locus_tag="QBF46_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDQ6672580.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     VMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28623..30110)
                     /gene="nusA"
                     /locus_tag="QBF46_000341"
     CDS             complement(28623..30110)
                     /gene="nusA"
                     /locus_tag="QBF46_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDQ6672581.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30138..30590)
                     /gene="rimP"
                     /locus_tag="QBF46_000342"
     CDS             complement(30138..30590)
                     /gene="rimP"
                     /locus_tag="QBF46_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDQ6672582.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30797..30873)
                     /locus_tag="QBF46_000343"
     tRNA            complement(30797..30873)
                     /locus_tag="QBF46_000343"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30837..30839),aa:Met,seq:cat)
     gene            31221..32564
                     /gene="argG"
                     /locus_tag="QBF46_000344"
     CDS             31221..32564
                     /gene="argG"
                     /locus_tag="QBF46_000344"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDQ6672583.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(32572..34197)
                     /locus_tag="QBF46_000345"
     CDS             complement(32572..34197)
                     /locus_tag="QBF46_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDQ6672584.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLLVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDCLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEKVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34656..34742)
                     /locus_tag="QBF46_000346"
     tRNA            complement(34656..34742)
                     /locus_tag="QBF46_000346"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34706..34708),aa:Leu,seq:gag)
     gene            complement(34757..35089)
                     /gene="secG"
                     /locus_tag="QBF46_000347"
     CDS             complement(34757..35089)
                     /gene="secG"
                     /locus_tag="QBF46_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDQ6672585.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35317..36654)
                     /gene="glmM"
                     /locus_tag="QBF46_000348"
     CDS             complement(35317..36654)
                     /gene="glmM"
                     /locus_tag="QBF46_000348"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDQ6672586.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36647..37495)
                     /gene="folP"
                     /locus_tag="QBF46_000349"
     CDS             complement(36647..37495)
                     /gene="folP"
                     /locus_tag="QBF46_000349"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDQ6672587.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37585..39519)
                     /gene="ftsH"
                     /locus_tag="QBF46_000350"
     CDS             complement(37585..39519)
                     /gene="ftsH"
                     /locus_tag="QBF46_000350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDQ6672588.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39619..40248)
                     /gene="rlmE"
                     /locus_tag="QBF46_000351"
     CDS             complement(39619..40248)
                     /gene="rlmE"
                     /locus_tag="QBF46_000351"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDQ6672589.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40374..40667
                     /gene="yhbY"
                     /locus_tag="QBF46_000352"
     CDS             40374..40667
                     /gene="yhbY"
                     /locus_tag="QBF46_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDQ6672590.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40823..41299)
                     /gene="greA"
                     /locus_tag="QBF46_000353"
     CDS             complement(40823..41299)
                     /gene="greA"
                     /locus_tag="QBF46_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDQ6672591.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41547..42980
                     /gene="dacB"
                     /locus_tag="QBF46_000354"
     CDS             41547..42980
                     /gene="dacB"
                     /locus_tag="QBF46_000354"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDQ6672592.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43166..44338)
                     /gene="cgtA"
                     /locus_tag="QBF46_000355"
     CDS             complement(43166..44338)
                     /gene="cgtA"
                     /locus_tag="QBF46_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDQ6672593.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44354..45319)
                     /locus_tag="QBF46_000356"
     CDS             complement(44354..45319)
                     /locus_tag="QBF46_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ6672594.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45446..45703)
                     /gene="rpmA"
                     /locus_tag="QBF46_000357"
     CDS             complement(45446..45703)
                     /gene="rpmA"
                     /locus_tag="QBF46_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDQ6672595.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45724..46035)
                     /gene="rplU"
                     /locus_tag="QBF46_000358"
     CDS             complement(45724..46035)
                     /gene="rplU"
                     /locus_tag="QBF46_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDQ6672596.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46294..47265
                     /gene="ispB"
                     /locus_tag="QBF46_000359"
     CDS             46294..47265
                     /gene="ispB"
                     /locus_tag="QBF46_000359"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDQ6672597.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47492..47770
                     /gene="sfsB"
                     /locus_tag="QBF46_000360"
     CDS             47492..47770
                     /gene="sfsB"
                     /locus_tag="QBF46_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDQ6672598.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47818..49077)
                     /gene="murA"
                     /locus_tag="QBF46_000361"
     CDS             complement(47818..49077)
                     /gene="murA"
                     /locus_tag="QBF46_000361"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDQ6672599.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49132..49386)
                     /gene="ibaG"
                     /locus_tag="QBF46_000362"
     CDS             complement(49132..49386)
                     /gene="ibaG"
                     /locus_tag="QBF46_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDQ6672600.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49546..49839)
                     /gene="mlaB"
                     /locus_tag="QBF46_000363"
     CDS             complement(49546..49839)
                     /gene="mlaB"
                     /locus_tag="QBF46_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDQ6672601.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49839..50474)
                     /gene="mlaC"
                     /locus_tag="QBF46_000364"
     CDS             complement(49839..50474)
                     /gene="mlaC"
                     /locus_tag="QBF46_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDQ6672602.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50493..51044)
                     /gene="mlaD"
                     /locus_tag="QBF46_000365"
     CDS             complement(50493..51044)
                     /gene="mlaD"
                     /locus_tag="QBF46_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDQ6672603.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51049..51831)
                     /gene="mlaE"
                     /locus_tag="QBF46_000366"
     CDS             complement(51049..51831)
                     /gene="mlaE"
                     /locus_tag="QBF46_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDQ6672604.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51839..52648)
                     /gene="mlaF"
                     /locus_tag="QBF46_000367"
     CDS             complement(51839..52648)
                     /gene="mlaF"
                     /locus_tag="QBF46_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDQ6672605.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52858..53835
                     /locus_tag="QBF46_000368"
     CDS             52858..53835
                     /locus_tag="QBF46_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDQ6672606.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53849..54835
                     /gene="kdsD"
                     /locus_tag="QBF46_000369"
     CDS             53849..54835
                     /gene="kdsD"
                     /locus_tag="QBF46_000369"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDQ6672607.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSHHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54856..55422
                     /gene="kdsC"
                     /locus_tag="QBF46_000370"
     CDS             54856..55422
                     /gene="kdsC"
                     /locus_tag="QBF46_000370"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDQ6672608.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYI
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55419..55994
                     /gene="lptC"
                     /locus_tag="QBF46_000371"
     CDS             55419..55994
                     /gene="lptC"
                     /locus_tag="QBF46_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDQ6672609.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55963..56520
                     /gene="lptA"
                     /locus_tag="QBF46_000372"
     CDS             55963..56520
                     /gene="lptA"
                     /locus_tag="QBF46_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDQ6672610.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56527..57252
                     /gene="lptB"
                     /locus_tag="QBF46_000373"
     CDS             56527..57252
                     /gene="lptB"
                     /locus_tag="QBF46_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDQ6672611.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57300..58733
                     /gene="rpoN"
                     /locus_tag="QBF46_000374"
     CDS             57300..58733
                     /gene="rpoN"
                     /locus_tag="QBF46_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDQ6672612.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58756..59043
                     /gene="hpf"
                     /locus_tag="QBF46_000375"
     CDS             58756..59043
                     /gene="hpf"
                     /locus_tag="QBF46_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDQ6672613.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59161..59652
                     /gene="ptsN"
                     /locus_tag="QBF46_000376"
     CDS             59161..59652
                     /gene="ptsN"
                     /locus_tag="QBF46_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDQ6672614.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59698..60552
                     /gene="rapZ"
                     /locus_tag="QBF46_000377"
     CDS             59698..60552
                     /gene="rapZ"
                     /locus_tag="QBF46_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDQ6672615.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60549..60821
                     /gene="npr"
                     /locus_tag="QBF46_000378"
     CDS             60549..60821
                     /gene="npr"
                     /locus_tag="QBF46_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDQ6672616.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            61035..61667
                     /gene="yrbL"
                     /locus_tag="QBF46_000379"
     CDS             61035..61667
                     /gene="yrbL"
                     /locus_tag="QBF46_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDQ6672617.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61664..62392)
                     /gene="mtgA"
                     /locus_tag="QBF46_000380"
     CDS             complement(61664..62392)
                     /gene="mtgA"
                     /locus_tag="QBF46_000380"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDQ6672618.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62389..63042)
                     /gene="elbB"
                     /locus_tag="QBF46_000381"
     CDS             complement(62389..63042)
                     /gene="elbB"
                     /locus_tag="QBF46_000381"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDQ6672619.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63272..65608)
                     /gene="arcB"
                     /locus_tag="QBF46_000382"
     CDS             complement(63272..65608)
                     /gene="arcB"
                     /locus_tag="QBF46_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDQ6672620.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLIHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65704..66633)
                     /locus_tag="QBF46_000383"
     CDS             complement(65704..66633)
                     /locus_tag="QBF46_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDQ6672621.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            67308..71768
                     /gene="gltB"
                     /locus_tag="QBF46_000384"
     CDS             67308..71768
                     /gene="gltB"
                     /locus_tag="QBF46_000384"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDQ6672622.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDALAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71781..73199
                     /gene="gltD"
                     /locus_tag="QBF46_000385"
     CDS             71781..73199
                     /gene="gltD"
                     /locus_tag="QBF46_000385"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDQ6672623.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMHGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGHIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73383..73862
                     /locus_tag="QBF46_000386"
     CDS             73383..73862
                     /locus_tag="QBF46_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDQ6672624.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            73879..74184
                     /locus_tag="QBF46_000387"
     CDS             73879..74184
                     /locus_tag="QBF46_000387"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6672625.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(74260..74724)
                     /locus_tag="QBF46_000388"
     CDS             complement(74260..74724)
                     /locus_tag="QBF46_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDQ6672626.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74721..75596)
                     /gene="nanK"
                     /locus_tag="QBF46_000389"
     CDS             complement(74721..75596)
                     /gene="nanK"
                     /locus_tag="QBF46_000389"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDQ6672627.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75593..76282)
                     /locus_tag="QBF46_000390"
     CDS             complement(75593..76282)
                     /locus_tag="QBF46_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDQ6672628.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76330..77820)
                     /gene="nanT"
                     /locus_tag="QBF46_000391"
     CDS             complement(76330..77820)
                     /gene="nanT"
                     /locus_tag="QBF46_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDQ6672629.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKSFSGAVPFGSAKNDLVKTKS"
     gene            complement(77930..78823)
                     /gene="nanA"
                     /locus_tag="QBF46_000392"
     CDS             complement(77930..78823)
                     /gene="nanA"
                     /locus_tag="QBF46_000392"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDQ6672630.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(78945..79736)
                     /gene="nanR"
                     /locus_tag="QBF46_000393"
     CDS             complement(78945..79736)
                     /gene="nanR"
                     /locus_tag="QBF46_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDQ6672631.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQATNKKK"
BASE COUNT        19728 a        21593 c        19826 g        18779 t
ORIGIN      
        1 ggttatatat gaataaaggt acaaaaatag ccattgcggg cttacttgcg cttttcgcag
       61 gtaatgccgc tgcaacagat ggtgaaatag tttttgatgg cgagatttta aaatccgctt
      121 gtgaaatcaa tgactctgat aagaaaattg aagttgctct tggtcactat aatgccgaac
      181 agtttcgttc tgttggcgat cgtagcccaa aaattccatt cactattcct ttagttaact
      241 gcccaatgac tgggtgggag cacgataacg gcaacgttga agcgtctttc cgtctatggc
      301 tagaaacgcg ggataatggt accgttccta acttccctaa tctggcaaaa gtaggctctt
      361 ttgctggcat agcggcaaca ggtgtgggta tccgtattga cgatgcggaa agcggaaaca
      421 ttatgccact gaatgctatg ggcaatgata acacggttta tcagatcccg gcggactcca
      481 atggtattgt caatgttgac ctcatcgctt actgcgtatc aaccgttgaa gcatcagaaa
      541 tcaccccagg ggaagcagac gctgtcgtta acgtaacgct ggattaccgt taatacgtta
      601 cggcgttatc tgacctgtca gataacgccc ttttccttcc tctttctcgt tgtatcaggt
      661 tgaaaaataa ctatgtcaaa acgaacattc gcggtgatat taaccttgtt gtgtagcttc
      721 tgtattggcc aggcgcttgc aggaggaatc gttttacagc gaacgcgagt gatctatgat
      781 gccagccgca aagaggctgc gttacctgtc gcaaacaaag gcgcagaaac gccttattta
      841 ctgcaatcat gggtagataa tatagatggt aaaagccgtg ccccatttat tataacccca
      901 ccgctatttc gtcttgaggc tggcgatgac tcatcactgc gaattattaa aacagctgat
      961 aacctgcctg aaaataaaga gtcgctgttc tacattaatg ttcgtgccat tccagcaaag
     1021 aaaaaatcag atgatgttaa tgctaacgag ttgacgctgg tatttaaaac acggatcaaa
     1081 atgttttatc gccccgcaca cctgaaggga cgggtaaacg atgcgtggaa atcactggaa
     1141 tttaaacgta gtgatcattc actcaatata tataacccaa ctgaatatta cgtcgtattt
     1201 gccggattgg cagtcgataa aatcgatctc acaagcaaaa ttgaatatat cgcgcccgga
     1261 gaacataaac agttaccact tcctgcatct ggcggaaaga acgtgaaatg ggctgcgatc
     1321 aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca aatataaaaa
     1381 acacaggtca tcagggaatg ccacaacgac accaccaggg acataaacgc acaccgaaac
     1441 agttggcgct cattatcaaa cgctgtttgc cgatggtgct cactggcagc ggcatgcttt
     1501 gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa accacaaaaa
     1561 gtggtatgca aacaaccgat ctgtcacgat tttcaaagaa atacgcacaa ctaccaggaa
     1621 cttatcaggt tgatatctgg ctgaataaaa agaaggtttc acagaaaaaa attacattta
     1681 ccgccaatgc agagcaactt ctgcagccac agtttacggt agaacaacta cgtgagctgg
     1741 gtattaaggt ggatgaaatc ccggcgctgg ctgaaaaaga tgacgatagc gtgatcaact
     1801 cgcttgaaca aatcattccc ggtacagctg ctgaatttga tttcaatcat cagcgactta
     1861 atttgagcat tccccaaatt gcactgtacc gtgatgcaag aggttacgtc tccccttctc
     1921 gttgggacga tggtatacca acgctgttta ccaactactc gtttacaggt tctgataacc
     1981 gttaccgcca gggcaatcgt agccaacgac agtacctgaa tatgcaaaat ggtgctaatt
     2041 ttggcccctg gcgattacgc aactattcca catggacacg caacgatcag acatcaagct
     2101 ggaataccat cagtagttat ttacaacgtg atatcaaggc attgaagtct cagttgcttc
     2161 tgggagaaag cgccaccagc ggcagtattt tttccagcta cacctttact ggcgttcaac
     2221 tcgcttccga cgataatatg ctgccaaaca gccagcgcgg atttgcccca acggtacgcg
     2281 gtatcgcaaa cagtagtgca atcgtgacta tcaggcaaaa tggttatgtg atctatcaaa
     2341 gcaacgtgcc agcgggtgcc tttgaaatta acgatctcta cccctcttcc aacagcggcg
     2401 atttagaagt cacgattgaa gaaagtgacg gtacgcaacg tcgctttatc cagccttatt
     2461 cttcattacc catgatgcag cgacctgggc atctaaagta tagcgcgacc gctggacgct
     2521 atcgcgctga tgcaaacagt gatagcaagg aacccgaatt tgctgaagcc acggcaatat
     2581 atggtttgaa taatactttt acgctgtatg gcggcctgct cggttctgaa gattattatg
     2641 cgctggggat cggtatcggc ggcacacttg gcgcactggg cgcgttgtcg atggatatca
     2701 acagagctga cacccaattc gataaccagc actcttttca tggctatcaa tggcgtacgc
     2761 agtacatcaa agatatcccg gaaaccaaca ccaatatcgc tgtcagctac tatcgctata
     2821 ccaacgatgg ctattttagt tttaatgaag ccaatacccg caattgggac tataacagtc
     2881 gccaaaaaag tgaaattcaa ttcaacatca gccagacaat atttgatggg gtaagtctgt
     2941 atgcctccgg ttcgcagcaa gactattggg gcaataacga taaaaacagg aatatctctg
     3001 ttggggtttc cggccagcaa tggggagttg gttacagcct gaattatcaa tacagccgct
     3061 acactgatca aaataatgac cgcgcactct ctttgaatct cagtattccg ttagaacgct
     3121 ggttaccgcg tagccgggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
     3181 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
     3241 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
     3301 atggaacctt cagtgccgga tacagctacg gtaatgacag cagccaatac aattacggcg
     3361 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
     3421 cttttgcgct tatcgatgct aatggggctt ctggcgtgag gatacaaaac tatccgggga
     3481 ttgctaccga tccctttggc tatgcagtgg ttccttatct cacgacttac caggaaaacc
     3541 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgagcaa acaacacagt
     3601 ttgtggtacc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
     3661 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
     3721 gcaacgatga gacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
     3781 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
     3841 gtcagtttgc ttttagtaca ccggattcag aacccacaac ctctgtatta caaggcacgg
     3901 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
     3961 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgatag cactagcggt
     4021 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
     4081 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     4141 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     4201 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     4261 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     4321 ataaaaaaca acacggtata ttctgctgca acccaaacgg gtgtttgcca gggtgatacg
     4381 tctcgttatg gacccgttaa tattggtgcg aacaccacct ttaccctgta tgtcaccaag
     4441 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     4501 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     4561 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaggtt
     4621 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     4681 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     4741 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     4801 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     4861 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     4921 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     4981 cagggcacag ccaccattac cgtcatcgtg cggtaaaaga agacgttgcg ttttgattaa
     5041 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     5101 cttcacgccg tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     5161 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     5221 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     5281 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     5341 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     5401 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     5461 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagagggtaa
     5521 acccgctgcg cttaacgcag tgatagcagc acacggcccg ggtagcggca ccacgcggat
     5581 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     5641 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc ttcgccagca gcgtttcagc
     5701 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     5761 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     5821 ctctaacgca cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     5881 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     5941 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     6001 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     6061 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggtacgg cgcaggctga
     6121 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     6181 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg tggagttgtt
     6241 taaccaacta ccgcaagaac tgaacgatgc tcagcgccgc gagaaaacac tgctggcggt
     6301 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     6361 acctgccgat ttagaacaaa accagcaagc ccgttactgg caggcaaaaa tcgatgccag
     6421 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgctcgg
     6481 cgcgaaagaa aagcagcaga atattgacgc cacctggcag gcgctctcct ccatgactca
     6541 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     6601 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     6661 cgactggcag aaacgttatc cgaacaaccc gggcgcgaaa atgctgccaa cgcagttggt
     6721 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccactgaa
     6781 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagcgg cgaaaaatat
     6841 cggcactcag ccagtggcag ctcaggtagc tgccgcacct gccgcagacg tagctgaaca
     6901 acctcagccg caaaccgtgg atggcgttgc cagcccggca caagcctcgg ttagcgatct
     6961 gaccggtgaa cagcctgcag cccagccggt gcctgtaagc gccccggcga caagcaccgc
     7021 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     7081 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggtcggtcc
     7141 gttgctgaaa aataacgttg aagagttgct gaagagcaac actccgctga acgtactggc
     7201 actgaaccag ccggagaata tcgaaaatcg cgtcaatatt tgttacttcg cgctttcacc
     7261 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     7321 ggtgctgatc ccacgcagtt cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     7381 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     7441 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctctcagagc
     7501 gacaaccgac tccggaatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     7561 ccagttcacc aataatggcg gtcgtgtcga tgcggtgtac attgtggcaa cgccgggtga
     7621 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     7681 gtacgccagc tcccgcagtg cgcaagggac cgctggcccg gatttccgac tggagatgga
     7741 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctaccgt taatgcagca
     7801 ggcactcagc gcggtgaata acgattattc actggctcgc atgtatgcga tgggcgtcga
     7861 tgcctggtcg ctggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     7921 cggtaatacc ggaagcctga cggctaaccc ggattgcgtg attaacagga acttatcatg
     7981 gctacagtac caacaaggtc aggtagtccc cgtcagttaa ccaccaaaca gaccggcgat
     8041 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     8101 aacgtgaacg agcgtggcgg cgagatcgct ctgataatgc gtgaaggccg gaccaccatt
     8161 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     8221 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     8281 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     8341 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     8401 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     8461 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     8521 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     8581 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     8641 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     8701 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     8761 cgatattgta aaagcggtag aagctgccgt tacgcgtgat atgaccattg tggcattgac
     8821 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caagatgtgg agatccgcat
     8881 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     8941 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     9001 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     9061 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     9121 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     9181 gattaagaaa gaagcgcgca ttaatgtaac ggcctatcag ggcaaagtgc tgctggttgg
     9241 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     9301 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     9361 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     9421 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     9481 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg tgaagcgggt
     9541 aactacggcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     9601 cgcgtcttat caggcctaca acgatacaaa ccgtaggtca gataaggcgt ttacgccgca
     9661 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     9721 cactgccacc atcgcccccg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     9781 aatcagtgac ggcaaactca ccgccgggag cgtcatcaac aatgccagcg ccggagcggt
     9841 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     9901 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     9961 atcggcatgg gggaataacc agacgcgagc ggcacccaac accagtactg caaggatgta
    10021 aaccgggatc gtactccaga aaagcgtcca tagcgccctg ccccagcggc taaaaaaccc
    10081 gccctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac
    10141 ccatttttgc accagcgtcg caatcagcaa caccatcacc agcccggcca ccagacgaat
    10201 cgccgcaaaa ccccagccga ggacaaagcc cataaacacc agcgtcgccg ggtttaacac
    10261 cggattgccc atccagaatg ccagcgcacc gcccatcgac acctgttggc gacgcattcc
    10321 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcaacgaaa acagcgttcc
    10381 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
    10441 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc ataccgcgag
    10501 gaaatagatc atcgcgtaat ccaacgccgc ctgccatggg ttagcatccg cctgcgcaag
    10561 gatagattta ccgatactgt gggtttcggc agcagtaaac gctttgccgt agtaaggttc
    10621 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
    10681 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
    10741 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
    10801 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
    10861 cgactctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
    10921 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgt tacgatcgcc
    10981 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcga ttaatgcttc
    11041 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatcgc
    11101 actgactacc aacatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
    11161 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
    11221 gagacaacaa aacacaatgt cgatgggatc ggtgacctgc gccaacgcgt cactcagttg
    11281 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
    11341 aatggcgtta actttcggtt cgttaatcaa catccgcagc aggtgaccgc ccaccagccc
    11401 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
    11461 cgctccacgg ctcatgcacc aggcttaata gaccatgagg taattatccc cgattgtggg
    11521 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
    11581 aatcactgat gaatttgagg attcagaatt tacttcaccc gcagacgagt tccgtaaagc
    11641 cggacacgaa gtgattacca ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
    11701 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgcctgcgg agtttgatgc
    11761 cctgctgcta ccgggcggcc attcaccgga ttatctgcgt ggggacaacc gttttgtcac
    11821 ctttacccgt gattttgtga atagtggcaa accggtgttt gccatctgtc acggcccgca
    11881 gttgctgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
    11941 tattgatgtt aaaaatgcgg gcgcggaatt ttacgatcag gaagtcgtgg ttgataaaga
    12001 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
    12061 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
    12121 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
    12181 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
    12241 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
    12301 ccgttccggc aactgccgcc tgcggcccgc tcatctgcgc gtggcgcgtt ttttcttccg
    12361 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
    12421 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
    12481 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
    12541 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
    12601 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
    12661 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgctccagt gggcgatcgt
    12721 tcgctggcgt tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
    12781 ctggtagcgg aaggcgcagg gtttgcagag ctgttaagca gtctgcaaac cccggagatt
    12841 aaaagcgatt gaaatgctcg tgatactcaa ccaggccggt aacgccattc agcgcgtcat
    12901 ccgctaaacg atgtacctgg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
    12961 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
    13021 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
    13081 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    13141 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    13201 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    13261 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    13321 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    13381 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    13441 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacagcccca gctccgtatc
    13501 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    13561 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg cgttctggct
    13621 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    13681 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    13741 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    13801 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    13861 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    13921 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    13981 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    14041 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    14101 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    14161 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    14221 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    14281 ggacggttac gcccgttggc agcgcgccgt ggatatggcg gcgcagctgg gtgccgacgc
    14341 gctgatcctc gccgacctcg ccatgctgga atacgccgcc gagcgttatc cgcatattga
    14401 gcgccacgta tcggtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    14461 ccattttgac gttgctcgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    14521 actggcacgg gtcacacctg taccactgga agtctttgct ttcggcagcc tgtgcattat
    14581 gtcggaaggt cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acaccgtagg
    14641 cgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    14701 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    14761 atgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    14821 tctcaatacc ctggaactgc tgccggagtt aatggcggcg aatattgctt cggtgaaaat
    14881 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc
    14941 tatcgaccgt tgtaaggccg atccgcaaaa ctttgtaccg caaagcgcgt ggatggagac
    15001 gctcgggtcg atgtccgaag gcacgcaaac cactcttggc gcatatcacc gtaaatggca
    15061 gtgagaaaag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    15121 ctggaagaat tttatcagca ggccgccacc agcagcgccg acgtgattta tcttggtgaa
    15181 gcggtatgca gcaagcgtcg ggcaaccaaa gttggcgact ggctggagat ggcaaaatcg
    15241 ctcgccggga gtggtaagca gattgtgctc tccacgctgg cgctggtgca ggcatcatct
    15301 gaactgggcg aactgaaacg ctatgttgag aacggtgagt ttctgattga agccagcgat
    15361 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    15421 aactgctaca acgcggtgac actgaaaata ttgctcaaac agggcatgat gcgctggtgt
    15481 atgccagtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    15541 ggcattcgta accagtttga agtggaagtc ctgagctacg gtcatctgcc gctggcctac
    15601 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    15661 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    15721 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    15781 atgcagggcc tggttgatgt ggtccgcctg tcaccgcagg gtactgacac tttcgcgatg
    15841 ctcgacgcct tccgcgctaa tgaaaatggc gctgcaccac tgccgttgac ggcgaacagc
    15901 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    15961 tcactttgtt aacaacttta actactcttt aatgcagtat taaagattaa tcggtaacaa
    16021 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccattc
    16081 ccgaaggttc ttcagcgcga gaagcattct cccactctct cgatctcgcc cgtctggctg
    16141 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgact ggcattgcca
    16201 gtgctgccac gtcggtattg atcggctatc tggcggcgaa taccaccacg ctgcatctgg
    16261 ggtctggcgg cgtgatgttg cctaaccact caccgttggt cattgcagaa cagttcggca
    16321 cgcttaatac actctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    16381 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    16441 gcgatgtggc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    16501 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagcg
    16561 cgcaactggc ggcgcagctt ggtctgccgt ttgcgtttgc ctcacacttc gcgccggata
    16621 tgctgttcca ggcgctgcat ctttatcgca gcaacttcaa accgtcagca cggctggaaa
    16681 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    16741 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    16801 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatggcgtgc
    16861 agcaggcgct gagtatgtcg ttggtaggtg ataaagcgaa agtgcgtcat ggcttgcagt
    16921 cgatcctgcg cgaaaccgac gccgatgaga ttatggtcaa cgggcagatt ttcgaccacc
    16981 aggcgcggct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    17041 gtgtcttaac gcgggaagcc gtatccgagc tggcaacgct gtcctacata gacctgataa
    17101 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    17161 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    17221 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    17281 aagcgtttac gcgatgcacg ggctaacagc gccggaacaa ttgccgccca gatggtagcc
    17341 gctaaaccag cataaccaat ggcgtacagg aatccgttcg ggaacaacag cccccccaca
    17401 actggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    17461 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccgaggaa cgaactcgct
    17521 accgcaaagt ttgagaacac gaccagcagc agatccagac tacggctgtt cagtacgccg
    17581 cttaacgcct gtaccagcac atcaatatta ccgcccttct ctgcaatacc gataaactcc
    17641 ggacgcggga tgttacccat cgtcgccagc aaccagatgg tatacagcgc cagcgccatc
    17701 agcgtaccgt acaccagaca tttcacgatg gttttcggat ctttgccgta atacttcatc
    17761 aggcttggca cgttaccgtg ataaccaaac gatgccagac agaacggcag ggtcatcaac
    17821 agatacggtg cataagacgc attgctttcg gcgacgttga acaatgtcgc aggctgcaca
    17881 tgccccagca ggctaccaaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    17941 gctgtcatgc gactgacggc tttagtgctc aaccacacca caaacgctac cagcaatgca
    18001 aaaccaaaac ccgccgcccg tgccgggacg tttagtgaca tctctgcgaa ggtgtgatgc
    18061 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    18121 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    18181 ctcgaaccga ttctgtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    18241 cagaaccagg taaagatcag cgccgccatt gaccagaaaa accacgcccc ggacatgacc
    18301 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    18361 agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc tctccagtga
    18421 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacacgagt acaaaagaca
    18481 gaaaaaaagc cccgatggta aaaatcgggg ctgtatatat tattttacag attgtgttcg
    18541 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga atcatcacga
    18601 cgcggagcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acggccacct
    18661 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gttcgccacc gaaaccacga
    18721 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    18781 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    18841 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccaat gtaacggctg
    18901 ctgatgtcgc cttcgttagc aatcgcacca acgatatgac gaacttcaac accatcatcg
    18961 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    19021 cgatcttcac ggtcaccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    19081 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggtacgat cagagtacgt
    19141 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    19201 tcagcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    19261 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    19321 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    19381 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    19441 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aactaccagg
    19501 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    19561 cgaccatctt tcaggcgttc cagagtctgt tcacgcagcg cctggttcat gtcaccgttc
    19621 agcgcggcgc tgttgtagcc gttacgctca agagcttctg ccacttccag agtcgcgttt
    19681 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    19741 gcttcgtttt tacgcatacc ccagacagtc cagtagctct ggctgatgtc aggacgggta
    19801 gtcacgctgg actgaatgcg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    19861 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    19921 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    19981 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    20041 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    20101 taacgctggc cgccgtacag ggcaaccacg tttacgccgc gcatgtgttt agagaaatcc
    20161 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    20221 ggtgctttca gctcaggatc aagattttgc agcagaggta aagagaatgc tgcagttttt
    20281 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    20341 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    20401 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    20461 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    20521 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    20581 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    20641 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    20701 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    20761 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    20821 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    20881 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    20941 agtgctgttt caacacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    21001 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    21061 gcgctaactt gtcacgaccg ccgtaatata atgcgatccc gcgattcaag tgcgcgtagt
    21121 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    21181 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    21241 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    21301 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    21361 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    21421 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    21481 cgaaacacca gcgcaaaaaa ggcttcattt accactcccg aagaccacgg ttgaatgaac
    21541 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    21601 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    21661 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    21721 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    21781 gtttgtcagc gatttgagag atgtggacca gaccttcttt accgccgccg atggcaacaa
    21841 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    21901 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    21961 cgatcttcac agtaccgtca tcttcgattt cgatggtggt gccggtttct tcggtcaggg
    22021 cacggataac agagccgcct ttaccgataa catctttgat cttgtccggg ttgatcttga
    22081 tggtatggat acgcggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    22141 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    22201 tctctttggt gataccttca attttgatat ccatctgcag tgcagagata ccgtcgcggg
    22261 aacctgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    22321 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    22381 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    22441 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    22501 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    22561 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    22621 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    22681 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    22741 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    22801 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    22861 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    22921 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    22981 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgtttagc gcttcgttta
    23041 ccggctccgg ctgccagtcc caacgcggtt taccggcttc tttcaccagt tcattgatgt
    23101 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcacagc atctggtctt
    23161 cgctcagcag ttgagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    23221 ccagatccag tttgctctct ttcagctcgt cctgagtcgg gttcagtacg tactggtcat
    23281 tgatgtaacc tacgcgggca gcaccaatcg ggccattgaa cggaatacca gacagagaca
    23341 gcgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    23401 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc gggcgaatcg
    23461 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    23521 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    23581 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaacg aataccgcgg
    23641 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    23701 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    23761 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    23821 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    23881 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    23941 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    24001 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    24061 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    24121 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    24181 ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    24241 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    24301 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    24361 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    24421 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    24481 ataccgataa atttgccgtt ctcaccttcc gtgacgcgaa ccagtccttc cagtggcgca
    24541 ccagatgtac gaaccgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    24601 accaccggat agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    24661 gcggctggaa tatcctgctg ttcagcttgc tcaacaagtt cacgcaggtg ctccagggtc
    24721 accatccgtt caaccggata tttacttacc gccagacggc gcaggtaaat aacatgcgcg
    24781 ccacagccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    24841 cagtgaattt ccagctccag ctcattgcct tcatggcgaa taaacagcaa ttcataaacg
    24901 gtaatcggac gcgcttcacg cggaacttca atgccctgac gcgcatattc gtacagtttt
    24961 ttgccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    25021 gtatccagtg ccgctgccag ctgctctgcg ctaaaggtta ccggacgttc ttcaacgatc
    25081 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    25141 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aatcggcaac
    25201 atgccggtcg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttatatata
    25261 cgtttcactt tttgcagcgc atcgttgctg gacataccct gaggtttatc cagcaacaaa
    25321 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    25381 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    25441 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    25501 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    25561 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    25621 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    25681 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    25741 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    25801 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    25861 tcacatcagg caatccatgc cggatgcagc gtaaacgcct tatcccgcat ggaaccctaa
    25921 aaaccttaag caatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    25981 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    26041 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    26101 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    26161 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    26221 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    26281 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    26341 gaggcatcag cacgtacgtt aaagccaacc aggatggcgt tggacgccgc agccagggtg
    26401 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    26461 gtagacagtt tcagcaagga gtcggagatc gcttcgacag aaccctgtac gtctgccttc
    26521 aggacgatat tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    26581 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atagagtgca
    26641 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggaaca
    26701 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    26761 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    26821 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    26881 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    26941 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cggtacccgc tttcgcagat
    27001 acgtgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    27061 tcgttcttaa cgcgatccgg atcagcttct ggtttatcga tcttgttcac tgcaaccacc
    27121 accggtacct gcgccgcttt cgcgtgctgg attgcttcga tggtctgcgg catcacaccg
    27181 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    27241 gaagtaaacg cggcgtgccc tggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    27301 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    27361 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    27421 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    27481 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    27541 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcactttg
    27601 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    27661 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    27721 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    27781 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    27841 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    27901 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    27961 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    28021 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    28081 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    28141 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    28201 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    28261 tccgcttcac gctgcgcttg ctcttccgct gcaaggcgtt cagcctcttg cggatcgcgt
    28321 ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg atttgctttt tccaccggta
    28381 ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg tcaatttgtc cgggcctgaa
    28441 tttttctgat tcaggtggtc aatcaaagtc tgtttctctt gtgcagacac agagtcgtca
    28501 gcagacttcc ggatacctgc atcagcaaat tgctgtacca ggcgttccac ggaggtctgt
    28561 cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc tgttccttcc tgctacagtt
    28621 tattacgctt cgtcaccgaa ccagcaaata ttacgggcag ccataatcag tgctccggct
    28681 ttttcgtcgg tcaacccttc gatatcagcc agatcatcaa tgccctgttc ggcgagatct
    28741 tccagcgtac aaacgccacg ggcggccagt ttgaatgcca aatcacgatc taccccttca
    28801 aggttcagca gatcgtcagc cggtttgtta tcaccgaggc tttcttcctg ggcctgtgca
    28861 atggtggcca gtgcattttt agcacgctcg cgcagtgctt caacggtcgg ctcatcaagg
    28921 ccttcgattt ccaacagctc tttcatcggc acataggcca gttcttccag cgtcgagaag
    28981 ccttcttcta ccagaacagt cgcgaagtct tcgtcgatgt cgagatattt ggtgaaggtg
    29041 tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca ggtcgtcaac ggtcatcacg
    29101 ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt tctgaccgtt acggccaatc
    29161 gcctgcgcca ggttaccggc ttcaacggcg atatccatgg tgtgtttatc ttcatccacc
    29221 acgatagaag caacgtctgc cggtgccatt gcgttaatca cgaactgcgc cgggttatca
    29281 tcccacagga cgatatcgat acgctcgcca cccagttcag tagacaccgc ctgaacacgc
    29341 gcgccacgca tacctacgca agcacctacc ggatcgatac gtttatcgtt ggttttcacc
    29401 gcgattttcg cacgagaacc cggatcgcga gccgctgctt taatttcaat cacttcttcg
    29461 ccgatttctg gcacttcaat acggaacagt tcgatcagca tttccggctt ggaacgagtg
    29521 acgaacagtt gcgcgccacg cgcttccggg cgaacggaat aaagcacgcc acgaacgcgg
    29581 tcgccagggc ggaagttttc acgcggcagc atatcttcac gcaggatcac ggcttcagcg
    29641 ttgttgccca gatccagaga gatgttgtcg cggtttactt ttttcaccac gccggtgatg
    29701 atttcacctt cgtgttcacg gaactgatca accaccatcg cacgttcggc ttcacgcact
    29761 ttctgcacga taacctgttt tgccgtctgg gtagtgatac ggtcaaaggt aacagactca
    29821 atctgatctt caacgtaatc gcccaggttc aggctttcat cttcataacg tgcggcttca
    29881 agggtgattt ccttggtcgg ctgggtgact tcatcaacaa ctaaccagcg acggaaggtg
    29941 tcaaaatcac cgcttttgcg atcgatctgt acgcggacgt cgatctcttg ttcatatttt
    30001 ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga aaatcttctc gcgaggtagc
    30061 gccttttcat tggatacggc ttcaactaca gccaaaattt ctttgttcat cgcgggcttt
    30121 tcacctcatc cagactatta aaagtgggga accaggttcg ccttctggat attactcagc
    30181 gcgaacactt catctttacc ttcgacggta actgtgatca tttcaccgtc taccgctttg
    30241 ataacgccct gccatttacg acggttttgt accgccatac ggagaaccag agtcacctct
    30301 tctccgacga aacgggcgta gtgttcagcc gtgaacagtg ggcgatcgag acccggtgag
    30361 gagacttcca ggttataagc aacggtgatg ggatcttcaa catccagcac agcacttacc
    30421 tggtggctca catcagcaca atcatcaaca ttgatgccat cttcactatc aatatagatg
    30481 cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccaa ccagttcaaa acccagggcc
    30541 tcaactggcg cagtaatcat ctctgttaat ttttgctcta atgtggacaa gcccaccccc
    30601 aagacataaa aaaagggcct aaagcccagt tattctgtag tcagataaca aaaaaccccg
    30661 ataaatcggg gctttatata actgaaccct ataaccgcaa ctgcggtctg gagcactttc
    30721 cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc ttcacagtat atttgaaaaa
    30781 ggactctaag ggaaagtggt tgcgggggcc ggatttgaac cgacgacctt cgggttatga
    30841 gcccgacgag ctaccaggct gctccacccc gcgcctgaaa cgtggcaaat cctactcgtt
    30901 ttgggtaaaa aatgcaaata ctgctgggat ttggtgtacc gagacgggac gtaaaatctg
    30961 caggcattat agtgatccat gccacatttt gtcaacgttt attgctaatc acgtgaatga
    31021 atatccagtt cactttcatt tgttgaatac ttttgccttc tcctgctctc ccttaagcgc
    31081 attattttat aaaaaacaca ctaaactctt cctgtctccg ataaaagatg attaaatgaa
    31141 aactcattta ttttgcataa aaattcagtg agagcggaaa tccaggctca tcatcagtta
    31201 attaagcagg gtgttatttt atgacgacga ttctcaagca tctcccggta ggtcaacgta
    31261 ttggtatcgc tttttccggc ggtctggaca ccagtgccgc actgctgtgg atgcgacaaa
    31321 agggagcggt tccttatgca tatactgcga acctgggcca gccagacgaa gaggattatg
    31381 atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa cgcacgtctg atcgactgcc
    31441 gcaaacaact ggtggccgaa ggtattgccg ctattcagtg tggcgcattt cataacacca
    31501 ccggcggcct gacctatttc aacacgacgc cgctgggccg cgccgtgact ggtaccatgc
    31561 tggttgctgc gatgaaagaa gatggcgtga atatctgggg tgacggtagc acctacaaag
    31621 gaaacgatat cgaacgtttc tatcgttatg gtctgctgac caatgctgaa ctgcagattt
    31681 acaaaccgtg gcttgatact gactttattg atgaactggg cggccgtcat gagatgtctg
    31741 aatttatgat tgcctgcggt ttcgactaca aaatgtctgt cgaaaaagcc tactccacag
    31801 actccaacat gcttggtgca acgcatgaag cgaaggatct ggaatacctc aactccagcg
    31861 tcaaaatcgt caacccgatt atgggcgtga aattctggga tgagagcgtg aagatcccgg
    31921 cagaagaagt cacagtacgc tttgaacaag gtcatccggt ggcgctgaac ggtaaaacct
    31981 ttagcgacga cgtagaaatg atgctggaag ctaaccgcat cggcggtcgt cacggcctgg
    32041 gcatgagcga ccagattgaa aaccgtatca tcgaagcgaa aagccgtggt atttacgaag
    32101 ctccggggat ggcactgctg cacattgcgt atgaacgtct gttgaccggt attcacaacg
    32161 aagacaccat tgagcagtat cacgcgcatg gtcgtcagtt gggccgtctg ctgtaccagg
    32221 ggcgttggtt tgactcccag gcgctgatgc tgcgtgactc tctgcaacgc tgggttgcca
    32281 gccagatcac tggtgaagtt accctggagc tgcgccgtgg gaacgattat tcaatcctaa
    32341 ataccgtctc agagaacctg acctacaagc cagaacgtct gacgatggaa aaaggcgact
    32401 cggtgttctc gccagatgat cgtattggtc aattgaccat gcgtaacctg gatatcactg
    32461 atacccgaga gaaacttttt ggttatgcca aaactggcct gctctcctcc tctgccactt
    32521 caggcgtgcc gcaggtagag aatctggaaa acaaaggcca gtaattcgac atcaaccctg
    32581 cccttcaggg gtggggttac cttgcttatc ctctgtaaac tcctttctga gcatcacata
    32641 atcgaaaaca tcatggtttg catccacaac ccgtgagtct cctttatagt gtgcaatcac
    32701 tttctcaagc gtttgcggct gttccggctt tgttaccccc atccacgcat taataaggta
    32761 attattgtaa gctgtcgaaa agatgttgtt ttccgttgta cgatccacgc catccccaag
    32821 aacggggcta taccagataa acatcgggac atgatatgcc tgctggctag cctccctgcc
    32881 tccatgaaaa tagacgttct tcttcgtagg gtcacgttcc aggccgtggt cggcaaaata
    32941 catgaccgag gcgcggcgat cttttaataa ttcaaaaacc tgtcctagca aacaatctgt
    33001 gtaatgaatg gagttgtcat agcaggcatc ctgatcgtcc tgcggttgaa acacggcgct
    33061 ggattgcgga taggcgctac aagccggttc atggcttcca tttaaatgaa gaacaatcag
    33121 tttcttttgc tgcgtatttt gctgtaatgc ttgcgataaa tgcggcaaca acaattcatc
    33181 aaatcctctg acataaactg tttccatggc gcgcatggcg atactggtaa ctgctgtacc
    33241 attctgccga aaagcggatt gcgaacttag ccagaaagtc tgaaatcctg cttgattagc
    33301 catattaata atgttgtcgg ggtaattatg aatgtcatgg ctcaaaacag aatcagcagt
    33361 taaagaaagc ggaaccgaca gcgcagtgta aggtgcgccg cttattgctt gattaaacag
    33421 tttgatctgt tttctttgtg cttcgacttg cggtgtcgta gagcgtgtat atccatacaa
    33481 agacatattg tcgacacgta cagactcccc cacaatcaac acgtaggtat caatacctgt
    33541 atccctgact aataattgaa aatacggcac cgtgtttgca attgagagta atctttgatg
    33601 ctctttcgct gctaaagcaa aatagttgag attgaaaaac ggcgtatagg ttgcaaatcg
    33661 cgacgctagt atatatggac tgaacgcttt tttatttttt gcatctttat aagcaaattg
    33721 acaagcggaa aataaactgc ccgaaatgac aatcagcaat aatattcctg tcactttttt
    33781 tgtcggcaag gaaacatcat attttattat taccgccaaa aaaagaaggg ataaaaaagc
    33841 aaaagcacat agataaggga tatacatccc cagcattttc acaacttcat ccggatcact
    33901 ctgcagcaca ctaatcgcaa atccatcatt gaaagttgta ccaaaggtac accatgaata
    33961 gaggctaata ctcatgtctg ccgcagtcag taacacaaat ggtgcggcaa caagcaggcg
    34021 aagcggaagg gatgatatac gtttaacaac caggatggtc agaacgaaga acagcaagcg
    34081 ctgaaccata tgcgctccgg aagaagcgac taagttcgta ataccaaaaa caataacaca
    34141 aagatacaac aaccaatgag atttaaaact tctagcaaat ttgttgaata ctgtcatcca
    34201 gaaatccaag ttcacaacca atataaacaa ttatcaacaa tgtttaattt tatatcagac
    34261 atgtttttca actctgatcc atatcagaac gaaacactgt tagttttttc tttttaaatg
    34321 atgcggccat tgcgttctca gtaattaaat aacattatca atgcgtctct aaacattacg
    34381 cagaacgtag ccatcagtcg ttatactgac aggagcagga tggtttatcg agaagtaaac
    34441 atatccgtat tcgtaccgga actggagttg acacaataaa gtgccaatta tgtcagtaga
    34501 agggaaaaat ttataactaa ggcgtaccgg caccatcgtt tcaaggtacc agctacgagt
    34561 aaagcaactg gacgagatac agatacctga caaccattcc tcagaccagg accaaaacga
    34621 aaaaagacgc ttttcagcgt ctcttttctg gaatttggta ccgaggacgg gacttgaacc
    34681 cgtaagccct attgggcact accacctcaa ggtagcgtgt ctaccaattc caccacctcg
    34741 gcacggatac tactttttag ttcgggatat cgctggtcgg cttagccgga gcagctggct
    34801 gagtttgttc ggttttcgcc ggtgcactca gattttccca ttcgctacct ttattggttt
    34861 tgttgctatt gatgttaccc agcaccagac tgatgatgaa gaataacgtt gccagcaacg
    34921 ccgtcatgcg ggtcatgaag ttaccagaac cacttgaacc aaacagcgta gcggaagcgc
    34981 ctgctccgaa ggaggctccc atatcagcgc ctttaccttg ctgcagcatg atcagaccaa
    35041 caaggccaat tgccacaata aggaaaacta ctaaaagagc ttcatacata atcaacctgt
    35101 tccttgcgga gttgccgcgt accaatgctt caaccaataa agcgggagtt tttaatcttt
    35161 cccactgaag cgggtgtgaa tactaaccaa agcgaatgac cttcgcaagg gcaattttat
    35221 cgcattgtat caactgcgga aaaaaacagc aaaaccgatg tgttcgctga gaaaaaaggc
    35281 gacaatcgcc gcctttttag ccagttatct aacgctttaa acggctttta ctgcatcggc
    35341 gatgcggtgt gcaaattcag tcacctgcgc ttcgtcttcg ccttccacca tcacgcgaat
    35401 taacggttcg gtgccggatt tacgcagcaa cacgcgtcca cggttgccca gcgcagcttc
    35461 aacctctgcg gtcacggctt taactgactc atgctcaagt ggatcgccgc tacctgcggt
    35521 gtaacgtacg ttaaccagaa tctgcgggaa cattttcatg ccgctgcaaa ggtcgtgcag
    35581 gctcatatgg ttacgtgcca tcgccgccag cacctgcaag ccagcaacga tgccgtcacc
    35641 ggtagtagtt ttatccagca ggatcacatg accggaattc tctgcaccga tacgccagcc
    35701 tttctcctgc attttttcca gtacgtagcg gtcacccact ttcgcgcgcg caaatggaat
    35761 tcctaactgt ttcagcgcca gttcaagccc catgttgctc atcaatgtac ccacagcgcc
    35821 accacgcagc tggccctgac gaagaccttc acgcgcgatg atatacatga tctgatcgcc
    35881 atcgacttta ttgccttcat ggtcaaccat aatcacgcga tcgccatcgc cgtcgaaggc
    35941 aataccgaga tccgcttttt cagccagcac acgagcctgg agcgcgcgaa cgtcggtagc
    36001 ccccacttcg gcattgatgt ttacaccgtt tggctcacaa ccgatagcga taacgttcgc
    36061 ccccagttcg cgcagcacgt tcggcgcgat gtgataagtc gcaccgtttg cacaatccac
    36121 cacaatcttc agttcactga ggctaagttc gttcgggaac gtggctttgc aaaactcgat
    36181 atagcgaccc gcggcatcaa cgatacggct ggctttaccc agttctgccg aatcaacgca
    36241 gctgatctcc ttttccattt ccgcttcgat ggcctcttct accgcatccg gcagtttggt
    36301 gccgtcgata gagaagaatt taatgccatt atcgtagaac gggttatgcg atgcagatat
    36361 cacaattccg gcctctgcgc ggaaggtacg cgtcagataa gccacggccg gtgttggcat
    36421 cgggccagtg aagagtgcgg aaaggcccgc tgccgccaga cccgcttcca gtgctgactc
    36481 cagcatatag ccagaaatac gcgtgtcttt accaataata atcttacggg agccgtggcg
    36541 cgccagcact ttacccgcgg cccaacccag cttaagcaca aaatcaggtg tgatcggcgc
    36601 atcccctaca cgaccacgaa tcccatcggt accgaaatat ttacgattac tcatagcgtt
    36661 tgttttcctt tgcagacaga gtggcttcca ccacccgcat cgcttctacg gtttctttga
    36721 cgtcatgaac acgaatgatg tgcgcgcctt gcattgcggc aatgaccgca caggccagac
    36781 taccgctcag gcgctcggac ggccccacgt tcagcagctg cccaatcatc gattttcgtg
    36841 acatacccac caacagcggc aggttgaaat ggtgaaattc agccaggcgc gccagtaatg
    36901 aatagttatg ggagagattt ttaccgaaac cgaatccggg gtcgagcaac aatttctctt
    36961 ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat aaagtagcga ttcacttctg
    37021 caaagacatc gtcatacttc ggagcttcct gcatggtttt tggatttccc tgcatatgca
    37081 tcagacaaac cggtaaaccg gtttctgcag ccgcctccag agcgccaggt tcggaaaggg
    37141 agcggatatc attaataatg tgagcgccaa ctttcgctga ctcacggatg acttctggtt
    37201 tggatgtatc gactgagatc cagacttcga agcgttgagc aattgcctca accacaggaa
    37261 taacacgttg caactcttct tcaacgctaa cttccgccgc ccctgggcgc gtggactcgc
    37321 caccaacgtc aatgatcgtc gcgccagcgt tgatcatcag attcgcatgt ttcaccgcat
    37381 ctatcagcga gttatgcgtg ccaccatccg aaaaggaatc aggcgtgacg ttgaggatcc
    37441 ccattacgtg aggatggcta aggtccagtg aagtaccctg ggcaaagagt ttcatgatgt
    37501 tatccctggt atgaattgat aagaaaaaac cccggagcac gccccggggt tttcggtaca
    37561 aatacagtca tctgatgcgg gaacttactt gtcgcctaac tgctctgaca tggtgttacc
    37621 cgggttcggc gtacgcggtt catcaaccgg acgaggagcc tttggactac cattgtcgcc
    37681 agaattgtta gaagcgcctg gttcttccca gcccgctggc ggacgtacat cgcgacgtgc
    37741 catcaggtca tcaatctgcg gtgcgtcgat agtctcatat ttcatgagag catctttcat
    37801 cgcatgcaga atatccatat tgtcggtcag aagctgacgc gcacgattat agttacgctc
    37861 aatcagtgct ttcacttcct ggtcgatgat acgtgcagtt tcatcggaca tatgtttcgc
    37921 tttcgctacg ctacggccga ggaacacttc accttcttct tccgcgtaca gcagtggacc
    37981 caatttctca gagaagcccc actgagtcac catgttacgt gccaggttgg tcgcaacttt
    38041 aatatcgttg gacgcaccgg tagatacatg ttccggcccg tagatgatct cttctgccag
    38101 acgaccaccg tacagcgtag aaatctggct ttccagtttc tgacggctgg cgctgattgc
    38161 gtcgccctca ggcaagaaga aagtcacacc cagcgcacga ccgcgtggga taatcgtcac
    38221 tttgtgcacc ggatcgtgtt ccggcaccag gcgaccgata atcgcatgac ccgcttcgtg
    38281 gtaagccgtc gattctttct gcgcttccgt catcaccatg gagcgacgtt ccgcacccat
    38341 catgattttg tctttcgctt tctcgaactc aaccatcgac acaacgcgtt tgttgccacg
    38401 agcagcgaac agtgccgctt cgttcaccag gttcgccagg tcagcaccgg agaaaccagg
    38461 agtaccacgg gcaatgattg ccgcgtcgat atcgggtgcc aatggtacgc gacgcatgtg
    38521 aactttcagg atctgctcac gaccgcgaac atctggcaag ccgaccacaa cctgacggtc
    38581 gaaacggcca ggacgcagca gggccgggtc gagaacgtcc ggacggttag tcgcggcgat
    38641 aacgatgata ccttcgttac cttcgaagcc atccatctca accagcatct ggttcagagt
    38701 ctgttcacgt tcatcgtgac caccgcccag accagcgcca cgctggcggc ctacggcgtc
    38761 gatttcatcg ataaagatga tgcacggtgc cgctttcttc gcctgttcga acatgtcacg
    38821 aacacgggat gcacccacac cgacgaacat ttctacgaag tcagaaccgg agatagtaaa
    38881 gaacggaact ttcgcttcgc ctgcaatcgc tttcgccagc agcgttttac cggtacccgg
    38941 aggaccgacc atcaagacgc ctttcgggat cttaccgccg agtttctgga agcggctcgg
    39001 ctcgcgcaga tactcaacca gttcagcaac ttcttctttt gcttcgtcgc agcccgcaac
    39061 gtcagcaaag gtcgttttga tctgatcttc cgtcagcatg cgcgctttgc tcttaccaaa
    39121 cgacatggca cctttgccac cgccgccctg catttgacgc atgaagaaga tccagacacc
    39181 aatcagcaac agcatcggga accaggagat gaagatagaa gccagcaggc ttggttcttc
    39241 aggcggttca ccgacaacct tgacgttctt ggtcaacagg ttatccagta atttcggatc
    39301 ctgaaccgga atgtaagtgg tataacggtt actatctttc ttggtaacgt tgatttcacg
    39361 tccgttgata cgcgcttcac gaacctggtc gttattgacc tcttgtagga aggtagagta
    39421 atccacctta cggccattag actcgctggg cccaaagctc tggaatactg acatcagcac
    39481 aacggcaatg accagccaga gtattaggtt tttcgccatg tcactcaagg gattaacctc
    39541 ttattacaac tgtgttaaaa acagcgtcag gatactctat atccagcatc tttcaaactt
    39601 tcgtctgaaa tctcccggtt agggtttacg cccggtcgct acaatataca cttcccgcga
    39661 acgtgcacga gaagagtccg gcttacgaac tttgaccttc gtaaacaggg agcgaatttc
    39721 ccttagatac tcatcgaaac cttcgccctg gaacaccttc actacaaaac tgccacctgg
    39781 cgctaataca tcacgacaca tttctagcgc cagttccacc agatacatgg cacgggggat
    39841 atccaccgcc ggtgttccgc tcatgtttgg tgccatatcg gacatgacaa cctggacttt
    39901 gctgtcgcca acgcgctcca gcagtgcttt catcaccagt tcatcacgaa aatcgccctg
    39961 aagaaagtcc acaccaacga taggatccat aggtaaaaga tcgcaagcga tgatgcggcc
    40021 tttgccgcca atttgggtga ccacatattg tgaccaacca cccggagcag caccaaggtc
    40081 gacaaccgtc attcccggtt taaagagttt gtcactttgc tgtatttcat caagtttaaa
    40141 ccaggcacgg gaacgtaacc cctttttctg tgcctgttga acatatttat cgctaaagtg
    40201 ttcctgaagc cagcggctgg agctggcaga acgcttctta cctgtcattt aactttccca
    40261 tggggataac tcatcgtaac caattgcgta aatttttacg cgcctatttg gtgatatatg
    40321 ggagatggcg gtagaatgac ccgttttcaa tcccaacgta agcaaaaaat acgatgaatc
    40381 tgagtactaa acaaaaacag cacctgaaag gtctggcaca tccgctcaag ccagttgttc
    40441 tgcttggcag taatggtttg accgaagggg tgctggccga gattgaacaa gcgttagagc
    40501 accatgaact catcaaggtg aaaatcgcca ccgaagatcg cgaaactaaa accttgatcg
    40561 tggaagctat cgtgcgcgaa accggcgcct gtaatgtaca ggtcatcggt aaaacgctgg
    40621 tgctttatcg cccaactaaa gaacgtaaaa tctcgctgcc acgctaagat tatcctaaag
    40681 ttacacacat ttgctgtgta aaacgagggg ttttccgcag gcaggagagc aaaatgccac
    40741 gctctgttcg ttgataaaag gccgcatagc ggcctttttc ctttctttac aatacatcaa
    40801 catcttgagt attgggtaat tcttacaggt attccacctt aattacttca aattctactt
    40861 cgccgcccgg cgttttgatg accacaacat catcttcttc tttgccgatc aggccacgag
    40921 caataggcga gtttacagaa atcaggtttt gtttaaagtc agcttcgtca tcgccaacga
    40981 tgcgataagt ctgttcttcg tcagaatcga gattcagcac cgttacggta gcaccaaaaa
    41041 taacgcgccc attgttgggc attttggtga catcaatcac ctgcgcgttt gacagcttgg
    41101 cttcgatgtc tttgatacgg ccttcgcaga agccctgctg ttcacgagct gcgtggtatt
    41161 cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc gatagcagca atgatttcag
    41221 gacggcgcac agatttcaga aaatccagct cttcgcgtaa tttttcagcg ccgcgtaagg
    41281 tcatcggaat agcttgcatt tgttatacct cttgaatatt cctgatgggg caagtcttca
    41341 cccaccccgg ctgttcggcc tgcccggcat aactcctcac cgggaccaga agcaaaaaaa
    41401 taccgacccg ggtacaagtc ccaggtcagc tacaattcac attttgatag tcattttacc
    41461 ctgaagttcc cgaagggtca tcgtttactt tatagggcgt tgcgccgtag tatgacggct
    41521 cgattccagg ttgttagcgc gagattatgc gattttccag atttatcatc ggattgacca
    41581 gctgtatagc gttcagtgtt caggccgcaa atgttgatga gtatattact caactccccg
    41641 ctggtgccaa ccttgccctg atggtgcaaa aagtcggcgc gtcggccccc gctattgatt
    41701 accacagtca gcagatggcg ctgcccgcca gtacgcagaa agtgattact gcgctggcgg
    41761 cgttgattca actcggcccc gattttcgtt ttaccacgac gcttgaaacc aaaggcaatg
    41821 tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt tggtgccgat ccgacgttaa
    41881 aacgtcagga tattcgcaat atggtcgcga ctttgaaaaa atctggcgtc aaccaaatcg
    41941 atggcaatgt gttgatagat acctccattt tcgccagcca cgataaagcc cccggctggc
    42001 catggaatga catgacacaa tgctttagcg ctccgcctgc cgccgccata gttgaccgca
    42061 actgtttctc cgtctcgctc tacagtgccc aaaaacccgg tgatatggcc tttatacgcg
    42121 tggcatctta ttaccccgtt acgatgttca gccaggtacg caccctcccc cgtggttctg
    42181 ccgaagcgca atactgcgaa ctggatgtgg tgccaggcga cctgaaccgc tttacgctga
    42241 cgggatgcct gccacaacgt tctgagccgc tcccgttggc ttttgccgta caggatggag
    42301 ccagctatgc cggggcaatt ctgaaagatg agttaaaaca ggcgggtatc acctggagtg
    42361 gaacactgct gcgccagact caggttaacg aacctggaac ggtagttgcc agtaaacagt
    42421 cggccccgct gcacgatctg cttaagatta tgctgaaaaa gtcagacaac atgatcgccg
    42481 atacggtttt ccgcatgata ggccatgcgc gcttcaatgt gcctggaaca tggcgggccg
    42541 ggtcggacgc cgtgcgtcag atcctgcgcc agcaagccgg tgtcgatatt ggaaacacca
    42601 ttattgccga tggttcaggg ctttcgcggc ataacctgat tgcccccgcc accatgatgc
    42661 aggtgctgca atacattgcc caacacgaca atgaacttaa ctttatctcc atgctgccgc
    42721 tggcgggcta tgacggctct ttgcagtacc gtgcaggtct gcatcaggcg ggcgtggatg
    42781 gcaaagtctc agcgaaaacc ggttcgttgc agggggtata taacctggcg ggattcatta
    42841 ccacagcgag cgggcaacga atggcgtttg tgcaatatct ttctggctat gcagtagaac
    42901 cggcggatca gcgtaatcgc cgtattccgt tagtgcgttt cgaaagccgt ttgtataaag
    42961 atatttacca gaacaattag tcaaaaagaa accccggcat atggccgggg tttcagatta
    43021 ttgacaaagt gcgctttgtt tatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    43081 atcgtgcaaa ttcaatatat tgcaatcatc ttgtaggcct gataagtgta gcgcatcagg
    43141 cttatttggc attggtcatt agtgattaac gcttgtaaat gaactcaacg ccttcttcgt
    43201 cgtcttcgtc ccagtcgtca tcccagtcct catcatcctc ttcagcaatc tcttcaagct
    43261 gctggcgatg ataatcatcc cacatgaatt cgactttctc tggctgtttc gcttcttcag
    43321 cctgcacgac cgggttttca atgataaagg tcatcacatc ccagcagaga tctttcacgc
    43381 ccagtccgct cgccgcagag atcagataat atttatcttc ccagcccagc gcctcagcga
    43441 tcgctttcgc tttctcttcg gcttctgcct tatccagcag atcgatcttg ttgaacacta
    43501 accaacgcgg tttcgccgcc agatcctggc tgtatttttc cagctcgctg ataataatac
    43561 gcgcgttttc aaccggatcg gtgccgtcaa tcggatcgat atcgatgagg tgcaacagga
    43621 cgcggcaacg ttccaggtgc ttcaggaagc ggatacccag acctgcgcct tccgcagcgc
    43681 cttcaatcag tcctggaata tcggcaacaa cgaagctctt ttcgttgtcc attcgtacca
    43741 cacccagact tggcaccaga gtggtaaacg gataatccgc cactttcggt ttagccgccg
    43801 ataccgcgcg aataaaggtc gatttccccg cgtttggcat ccccaacata ccgacgtcag
    43861 ccagcagcat cagctccagc agcagctcac gcttatcacc cggcgtaccg ttggttttct
    43921 gccgtggtgt acggttaacg gacgatttga aacgggtatt gcccagaccg tgccagccgc
    43981 ccttagcaac cagcagacgc tgaccgtgtt tagtcatatc gcccatggtt tcaccagtac
    44041 cctggtcgat tacacgcgta cctaccggca ctttaatcgt cacgtcttta ccgcgcttac
    44101 cggtacaatc gcggcttgcg ccgttctgac cgcgctctgc acggaaagat ttttcaaaac
    44161 gataatcgat aagcgtgttc aggttttcgt cggcttccat ccatacgtca ccaccatcac
    44221 caccgtcgcc gccatccggg ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc
    44281 aaccattacc gccatcacct gcaacgacca gaatcgatgc ttcatcaaca aacttcattt
    44341 tattctccgt aaatcattcg cctgcgcggg gttgcgatac caccgttgta tgcttacgta
    44401 atccgcccca aatacgatga ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg
    44461 caccgagata acctaaaagg tttaacatcg gtctggcgaa gaaatcgggc caggccagtg
    44521 ataaaagatc tgaaaaaaac agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct
    44581 gcgctgcctg ccagcgagcc atcgcttccg ccagggcgcc atatcctacc aatgtattca
    44641 gtccgcaaaa aattaaacat gcgagctgcc agtggctaag ctgcgctatc actccaggct
    44701 ttgccagagg gaagagcgcg attgtacata aagtgtacag caaaaacagg atctgcggtg
    44761 aggccagccg acgcaataaa accttttgcg ccacgccata actcacccaa accgtcgccg
    44821 caccgacccc aaagatgact ccccaggtgt aatcagtgag ctttgtaaat atctcgacca
    44881 gactggtgtt aaaaaacatc accaggccgc tcaggagcat caatgcccct acaacctgag
    44941 tgctgcgcat tttctctttc aggataaata cgctggcaac catcatgcca actggcgaga
    45001 gttgcccaat cacctgcgaa gcggtcggac tcaggtattg caaggatgag ctgaacagga
    45061 tgaagttccc aaacagcccg gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac
    45121 gaaacacgcg taatggcggc aacctcttct tcaccgcaag aatggcaccc aggccaatac
    45181 tcgccatcaa gaaacggtaa aacacgattg tcggaggttc catcacctcc agcacctgct
    45241 tcattgcgat tggcaacgcc ccccagcaaa ttgctgtggt gagcgccaaa agaatgccaa
    45301 tgcctgcctg ctgcttcata ccgtattccc tgcaaagcgc atttgcggat taacgtcgca
    45361 ccatgctcat ttttaccggt tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt
    45421 catccgttac cgggacgcga aaaacttatt cagcttcgat gctgataaat ttacggtttt
    45481 tcgggccttt aacttcgaat ttcactttac cgtctgcttt agcaaacaga gtgtggtcac
    45541 gaccgcaacc tacgttagcg ccagcgtgga atttggtacc acgttgacga acgatgatgc
    45601 tacccgccag aacggattcg ccaccgaaac gcttaacgcc caggcgttta gcttctgaat
    45661 cgcgaccgtt acgtgtggag ccgccagcct ttttatgtgc catttgaaat ctctcctcag
    45721 gtcttaggcg ctgatgccag taattttcac atcagtgaac cactgacgat ggccctgctg
    45781 cttacgatag tgtttacgac gacgaaactt aacgatttta actttctcgc cacgaccgtg
    45841 agcaacaact tcagctttga ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc
    45901 ttcaccgttt gcgatcatca gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc
    45961 cagcttttcc aggcgaacgg tctgaccttc gcttactcgg tgttgtttac caccactttg
    46021 gaaaaccgcg tacatataaa actccgcttc cgcgcacacc ttttcaatga ttcagagtgc
    46081 gctataaata ttcacaatag ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc
    46141 tacagtcaat acacgaagaa aaaaaacaca acttgtacgg taacgtttat ctgtgccatt
    46201 ttttcagtac aatcacccta tattcctaac cataaaccct aagttgcctt tgttcacagt
    46261 aaggtaatcg gggcgaaaag cccggctttt gcgatgaatt tagaaaaaat caatgagtta
    46321 accgcgcaag atatggcggg tgttaatgcg gcaatccttg agcagcttaa ttccgacgtc
    46381 caactgatca atcagttagg ctattacatc gtcagcggcg gcggtaaacg tattcgtccg
    46441 atgattgctg tactggctgc acgagctgtt ggctatgagg gaaatgcgca tgtcaccatt
    46501 gctgccttga tcgagtttat ccacacggcg actctgctac acgacgacgt tgtggatgaa
    46561 tcagatatgc gcaggggtaa agctaccgcc aacgctgcat ttggcaatgc cgccagcgtg
    46621 ctggtaggcg attttattta tacccgcgcg ttccagatga tgaccagcct cggttcgctc
    46681 aaagtgctgg aagtcatgtc agaagccgta aacgtcatcg cagaaggtga agttctgcaa
    46741 ctgatgaacg ttaacgatcc ggacatcact gaagaaaact acatgcgcgt tatctatagc
    46801 aaaaccgcgc gtctgtttga ggctgccgcg cagtgttccg ggattctggc tggctgtacg
    46861 ccggaggagg agaaaggcct gcaggattat gggcgctatc tcggcactgc tttccagttg
    46921 atcgacgatt tactcgatta caatgccgat ggcgaacagt taggtaaaaa cgtcggcgac
    46981 gatctgaacg aaggtaaacc gacgctgccg ctgctgcatg cgatgcatca tggcacacca
    47041 gaacaggcac agatgatccg taccgccatc gaacagggta acggtcgcca tcttctggaa
    47101 ccggttctgg aagcaatgaa cgcttgtgga tctcttgaat ggacgcgtca gcgcgccgag
    47161 gaagaagcag acaaagccat cgcagcgtta caggtgctcc cggacacccc ttggcgagaa
    47221 gcactcatcg gcctcgcgca catcgctgtt caacgcgatc gttaatctcc tcccctcatc
    47281 ccgcgcagcg cgcggggtgg ttccagtaaa ttccataaaa acacttactc agctctataa
    47341 cacctgaaaa acaggctgtg catctttata tattctgaat attcacactc cttacaggaa
    47401 ctttttagag caatcggcca tcaggagtat agtgatgctc gacagaagaa gtgttctgaa
    47461 tgaaagcgac taacttaagg agtgaggaaa gatggaaagt aatttcattg actggcatcc
    47521 cgctgacatc attgcgggtt tgcgcaagaa gggaacgtca atggcggcgg agtctcgcag
    47581 aaatggtttg agttcctcaa cgctggcgaa tgcattatcg cgcccatggc cgaaaggaga
    47641 gatgattatt gcgaaagccc tgggaactga cccctgggtt atctggccat cacgctacca
    47701 tgatccgcag acccatgagt ttatcgacag aacgcagttg atgcgtagct acactaaacc
    47761 gaaaaaatga gtggtctggc ggtagccccg cgaacggggc tgccagctct cagacgatta
    47821 ttcgcctttc acacgctcaa tatttgcacc taaagcgcgc agtttgtctt caatgcgttc
    47881 gtagccacga tcgatgtgat aaatacgatc aaccaccgtc gtcccttccg caatacagcc
    47941 agccagcacc aggcttgctg atgcacgcag atcggttgcc ataacctgtg cgccagaaag
    48001 tttttcaaca ccgtgacaaa taacggtatt gctttcgatt tcggcatgcg cgcccatacg
    48061 gctaagctcc ggcacatgca taaagcggtt ttcaaagacc gtttcggtga tgaatccggt
    48121 cccttctgcc accaggttca acagcgtgaa ctgggcctgc atatcggtcg ggaatgccgg
    48181 atgcggcgcg gtacgtacgt taacagcctt cggacgtttg ccatgcatat ccaggctaat
    48241 ccagtcttcg ccgacttcga tgtccgctcc agcatcacgc agtttcgcca gcacggcgtc
    48301 cagagtatct ggctgcgcgt tacggcagat aattttgccg cgagaaatcg ccgccgccac
    48361 caggaaagta ccggtttcga tacgatccgg tagcacgcga tagacaccgc cgcctaaacg
    48421 ttccacacct tcgatgacga tacgatcggt gccctgaccg ctaattttcg cacccagcgt
    48481 aatcaggaag ttcgcggtat cgacgatttc cggttcacgc gctgcgtttt caataatcgt
    48541 ggtaccttcc gccagggttg cagcacacat gatggtcacc gttgcgccaa cgctgacttt
    48601 atccatcacg atatgtgcgc ctttcagacg accatcgacg gaagctttaa cgtaaccttc
    48661 ttccagtttg atggtcgcgc ctaattgttc gaggccagaa atgtgtagat caaccggacg
    48721 cgcaccgatc gtacaaccgc caggcagtga aacttgcccc tgaccaaagc gcgctaccag
    48781 cggccccagt gcccagatag aagcacgcat ggttttaacc agatcgtaag gtgcgcagaa
    48841 tacattaacg tcgcgggcat caatatgcac agaaccatta cgttctactt tcgcacccag
    48901 ctggcttagc agcttcattg atgtatcgac gtctttcagt ttcgggacgt tctggatctc
    48961 taccggttct tccgccagta gagcggcaaa aaggataggc agagcagcat ttttagcgcc
    49021 ggaaattgtg acttcgccct ggagcttcgt tggcccctga acacgaaatt tatccattta
    49081 gtttgttctc agttaacaat tcatatccgc taccggcgaa tcgcccatag ctcaaaagcc
    49141 gttcagtttg cgatcgcgcg cccactccgc aggggtatac gctttgatcg acacagcatg
    49201 aatgcggtta tccgcaatat attccatcag cgggccatag accgtctgct gttttttaac
    49261 ccgactcatg ccgtcaaaca actcacccac ggcaataacc tgaaagtggc tgccatcgcc
    49321 ggaaacgtgg acttcctgga gggagagagc gttcatcaac acgctctgaa tttcattatt
    49381 ttccatggga tcttcaatca tcagttaata aaccagcgaa acatcttaga gcaaagttgc
    49441 gctggcataa ataagcaaaa agcctcgctg ataaatcaga caaggctcga cttgcaggca
    49501 ggtttgccgg acaggcggtt aacgccatat ccggcctgaa aaaatttaac gaggcagaac
    49561 atcagcaggc aaattataca atttcgccag ggtatacact ttgtcgttta ccccctgaag
    49621 cgtcacattg ttgccctgct ttttcgccag atcgataaga tggagcagca gtgccagtcc
    49681 ccccgtatcc acgcgggaca cacggctaag atcgatgcag gtaaccccct tcaccgcttc
    49741 ctcacgcatt tcccaaagcg gtagcagaac gtcctgatcc agctctccgg ataacgccag
    49801 cgtgtcaccc gtctgcatcc agctcagtga ctcgctcatt attttttctc ttccagagtg
    49861 attttctgtt gagaaatcga tttcagttgc gcagtcaggc cgtcgatacc tttggtacgc
    49921 agcagcgttc cccactcgtt ttgtttggtg gtgatcatac tgacgccttc agcaatcatg
    49981 tcgtaagcct gccagtttcc cgtctgggag tttttacgcc actggaagtc cagacgcacc
    50041 ggcgggcggc cattcggatc aataatggta acgcgaatag gcacaatggt tttatcaccc
    50101 agcggctgtt ctggcgcaat ctgataggtt tgaccgtgat acatcgccag cgcctgaccg
    50161 taagcctgct tcaggtactc acggaaagcg gcaaagtaag catcacgttg agctggggtc
    50221 gcactcttgt aatactggcc cagcaccagc gcaccggcgt atttcacctg tacgtatggc
    50281 agcagttcct gatcaacaat ggtacgcaga taatccgggt tggcccgaat ttgcggttgc
    50341 tcattcttca ggcgatcgaa cgttttctgc gccgcctcgt ccatcagctt atacggattg
    50401 gtttggtctg ccgcggttgc cgcactcagc ggtgcaatca ccagcaaagc gaccatcatt
    50461 aaacgtttaa acatgcgtcg gttctcctga aattatttcg ttgtacccac aggttcagtg
    50521 gtttcattat tacctggcgc agcagctggc gcatcgccac tattcttatt gtcatcgcct
    50581 ttactaccgt aaaggaactg accaatgaga tcttccagca ccatcgcaga cttggtgtcc
    50641 tgaattgtat cgccatcctt caggatagca gtccccagtt ccgggtcttc aaaaccgacg
    50701 tttaatgcca gatattgttc tcccagcagg ccggaagtac gaatgctcag cgaactggta
    50761 tcaggaatgt ggttataacg ttgttcaatt tccagcgtta cgcgtggcag ataggttttc
    50821 gggtccagcg taatatccgc cacccggccc acaacaacgc caccaatact gaccggagag
    50881 cgcgctttca ggccgccaat gttatcgaac gtcgcataaa gcgtgtaggt cggttcagta
    50941 cgtatggacg tcacgttcgc cgccttcagg caaacaaaca gcgccgccag cagtgctgct
    51001 aataaaaaga tacccaccca aatttcattt tttttcgttt gcatgaactc aattcccaaa
    51061 catcaatgcg gtcagcacaa aatccagccc cagaacagcc agagacgagt ggacaacggt
    51121 gcgagtggtt gcccggctaa tcccggcaga cgtcgggatg gcgtcgtagc cgttaaacaa
    51181 cgaaatccac gtcaccgtga tggcgaacac cacgctctta atcagacagt tgaccagatc
    51241 catacgccag tcgacggcat tttgcattgc cgaccagaag aacccgctat caatgccttt
    51301 ccaactgacg ccgaccagag atccgcccca gatccccacg gcgacgaaga taaccgtcaa
    51361 cagtggtaat gaaataaccc cagcccagaa acggggagaa ataacccgac gcagcggatc
    51421 caccgccatc atctccatac tggagagttg ctctgtagcg cgcatcaggc cgatttctgc
    51481 ggttaacgcc gaaccagcac gcccggcaaa caacagcgcg gcaaccaccg gccccagttc
    51541 acgcagtagc gataacgcca ccagcatacc cagactggtt tccgcactat aagtggtcag
    51601 aaccagataa ccttgcagcc ccaacaccat tccgatgaac acgccagaaa ctacaataat
    51661 cagcatcgac aggacgccga cattatagag ctggcgcacc agcagaggcg catgtttgcg
    51721 aaattccggt ttgccgacca gcgcattgaa taacattaac ccggcccgcc cgaacgttct
    51781 cagggtttta atccctttat gtccgagcga cgccagcgca tttaacagca tgagtggctt
    51841 aactccctgg taaaagatca gcgtgataat cgccggcagg atagcggaac ggaacaggcc
    51901 cgtcagctat cccgtccaga aactgacgta cgcgcggatc aggattcgcc tgcaacgcct
    51961 gggcactgcc atgagcgaca atttttttgt ccgccaggat ccaggcgtga tccgcaatac
    52021 ttaacacttc cggcacatcg tgagaaacca ccacacaagt cacgcccagc gcactgttca
    52081 gctcagaaat cagcttcacc agtacgccca tggtgatggg atcttgccca acaaaaggtt
    52141 catcaaacat gataagatcc ggctccagcg caatcgcacg cgccagcgct gcacgccgcg
    52201 ccatcccacc ggaaagttca gaaggcatta gcttagccgc tccacgcagc cccacggcct
    52261 ccagcttcat catcaccgta ctatgcaaca acggcgcggg aagttgggta tgttcgcgca
    52321 gtggataggc gacgttgtca aatacgttca tatcagtgaa caacgccccg gactgaaata
    52381 acatgctcat ccgtttgcgc actgtataca ggcgcgaacg agacatcgcc ggaatattct
    52441 caccatcgaa aaggatctca ccatgatctg gtgcgatttg cccgccaatc agacggagta
    52501 gcgtcgtttt accgatgccc gatggcccca tgatcgccgt gatcttccct cgcggcacgg
    52561 tcagggaaat attatcgaag atgcagcgat tgccacgcgt aaaactgaca tcgcgcatat
    52621 cgactaaatt cgccacagac tgctccataa ttcacccttc gtcttgcgtt gattttctaa
    52681 gcatggcgct caatttaacc ttgaacccaa catatttaca gaatattacc cgccgtggtt
    52741 agcgaaagct ggcatttgtt ttacttttta gccgcataaa gtcaaaatta agaatccgtt
    52801 acggctttct gaaaatcttc agcggaccgg cgagtatacc tgaagaaagg acgttagatg
    52861 cttttagcta cggcactgtt aattgttggt ttacttttgg tcgtttacag tgccgaccgc
    52921 ctggtttttg ccgcgtctat tctttgccga acctttggca tcccgccgct gatcatcggc
    52981 atgacggtgg tcagtattgg tacatcgtta ccagaaatca tcgtctcgct tgccgcgtct
    53041 ctgcacgaac aacgcgattt agccgttggt acagccctcg gctcaaacat tatcaatata
    53101 ttgctgatcc tcggtctggc tgcgctggtt cgtcctttta ccgtccattc tgatgttcta
    53161 cgccgtgaat tacccttaat gttgttggtc agcgtggtgg ccggttccgt actctatgac
    53221 ggacaactta gtcgaagcga tggtatcttt ctcctctttc tggctgtgct atggctgctg
    53281 ttcattgtta aacttgcacg tcaggctgaa cgtcagggga ctgacagcct gaccagagag
    53341 cagcttgcag agctgccgcg tgacggcgga ttgcccgtcg cgtttttatg gctcggcatt
    53401 gcgcttatca tcatgccagt ggccacgcgg atggtggttg ataacgccac ggtgctggcg
    53461 aattactttg ccatcagcga gttgacgatg ggtctgacgg caattgctat cggaaccagc
    53521 ctgccggaac tggcaaccgc aatagcgggg gttcgcaaag gtgaaaacga cattgctgtc
    53581 ggaaatatca ttggcgcaaa catttttaat attgtcatcg tgttgggttt acccgcgctg
    53641 ataacgccag gagagattga tccattggcg tacagtcgtg actacagcgt gatgttgctg
    53701 gtgagcatta tttttgcgtt gctgtgctgg cggcgctccc cgcaaccggg ccgtggtgta
    53761 ggggtattat taactggcgg atttatcgta tggctggcga tgttgtactg gttatcgcca
    53821 atactcgttg aataactgga aacgcattat gtcgcacgta gagttacaac cgggttttga
    53881 ctttcagcaa gcaggtaaag aagtcctggc gattgaacgt gaatgcctgg cggagcttga
    53941 tcaatacatc aatcagaatt tcacgcttgc ctgtgaaaag atgttctggt gtaaagggaa
    54001 agttgtcgtc atggggatgg ggaaatcggg gcacatcggg cgcaaaatgg ccgcaacgtt
    54061 tgccagcacc ggtacacctt catttttcgt ccatcctggt gaagccgcgc atggtgattt
    54121 aggcatggtc accccacagg atgtggtgat tgctatctct aactcaggtg aatccagcga
    54181 aatcacggcc ttaattccag tgcttaagcg tcttcacgta ccgttaatct gcatcaccgg
    54241 tcgcccggag agcagcatgg cgcgcgccgc agatgtgcat ctgtgtgtta aagtagcgaa
    54301 agaagcctgt ccgttagggc tggcaccgac cagcagcacc accgccacgc tggttatggg
    54361 cgatgccctc gctgtcgcgc tgttaaaagc acgcggcttt actgctgaag attttgcgct
    54421 ctcacaccca ggcggcgcac tgggtcgtaa acttctgctg cgcgtaaacg atattatgca
    54481 tacgggcgat gagatcccgc atgttaagaa aacggccagt ctgcgtgacg cattgctgga
    54541 agttacccgc aaaaatcttg gtatgactgt catttgcgat gacaatatga tgattgaagg
    54601 catctttacc gacggtgatt tacgccgtgt cttcgatatg ggcgtggatg ttcgtcagtt
    54661 aagtattgcc gatgtgatga cgccgggggg aatacgcgtg cgccctggca ttctggccgt
    54721 tgaggcactg aacttaatgc agtcccacca tatcacctcc gtgatggttg ccgatggcga
    54781 ccatttactc ggtgtgttac atatgcatga tttactgcgt gcaggcgtag tgtaaagatt
    54841 caaggataaa caacaatgag caaagcaggt gcgtcgcttg cgacctgtta cggccctgtc
    54901 agcgccgacg ttatggcaaa agcagagaac attcgtctgc tgatcctcga cgttgatggc
    54961 gtactgtcag atggcctgat ttatattggc aataacggcg aagagctgaa agcgttcaat
    55021 gttcgtgacg gttatggcat tcgttgtgcg ctcacctctg atattgaagt cgctatcatt
    55081 accgggcgaa aggctaaact ggtagaagat cgttgtgcca cattggggat cactcacttg
    55141 tatcaggggc agtcaaacaa actgatcgcc tttagcgatc tgctggaaaa actggcgatt
    55201 gccccggaaa atgtggctta tgtcggcgat gatctcatcg actggccggt aatggaaaaa
    55261 gtgggtttaa gcgtcgccgt ggccgatgcg catccactgt tgatcccgcg cgccgattac
    55321 gtgacgcgca ttgctggcgg tcgtggcgca gtgcgagaag tttgcgactt attactcctg
    55381 gcgcagggca aactggatga agccaaaggg caatcgatat gagtaaagcc agacgttggg
    55441 ttatcattgt gctatcactg gcggttctgg tgatgatcgg cattaatatg gccgaaaaag
    55501 acgataccgc ccaggtggtc gtcaacaaca atgatcccac ctataaaagc gagcatacgg
    55561 acacgctcgt ctataaccca gaaggggcac taagctatcg attgattgct caacacgttg
    55621 aatattattc cgatcaggcc gtttcgtggt ttacgcagcc ggtacttacc acgtttgata
    55681 aggataaaat cccgacatgg tccgtaaaag cagataaagc caagctgacc aatgaccgga
    55741 tgctctattt atatggacac gttgaagtca acgcacttgt gccggactct caacttcgca
    55801 gaatcacgac ggataacgcg cagatcaatc tggtgacgca ggatgttacc tctgaagacc
    55861 tcgtcacgtt atacggaaca acatttaact ccagcggtct gaaaatgcgc ggcaacttac
    55921 gcagcaagaa cgccgagctg attgaaaagg ttagaacatc ctatgaaatt caaaacaaac
    55981 aaactcagcc ttaatcttgt gcttgccagc tcacttctgg ccgccagcat tccggcattt
    56041 gccgtaaccg gagacactga tcagccgatc cacattgaat cggaccagca atctcttgat
    56101 atgcaaggca acgtggttac ctttaccggt aatgtcatcg tcacccaggg caccatcaaa
    56161 attaatgccg acaaagtggt cgttacccgt ccgggcggcg aacaaggtaa agaagtgatt
    56221 gacggctacg gtaaaccggc aacgttctac cagatgcagg acaacggtaa acccgttgaa
    56281 ggtcacgctt cccagatgca ctacgaactg gcaaaagatt ttgtcgttct gacgggtaat
    56341 gcttatctgc agcaggtcga tagcaacatt aagggcgata agatcactta cctggtgaaa
    56401 gagcagaaaa tgcaggcttt cagcgacaaa ggcaagcgcg taacaaccgt tctggtgccg
    56461 tcgcagctgc aggacaaaaa caacaaaggc cagaccccgg cacagaagaa gggtaattaa
    56521 ttcgttatgg caacattaac tgcaaagaac cttgcaaaag cctataaagg ccgtcgcgtg
    56581 gtagaagacg tcagcctgac cgtcaactcc ggggaaattg tcggtctgct ggggcctaac
    56641 ggtgccggta agaccaccac tttctatatg gttgttggca ttgtgccgcg cgatgcgggc
    56701 aacatcatta ttgatgatga cgatatcagt ctgctgcctc tgcatgcacg cgcgcgccgc
    56761 ggtatcggct atctgccaca ggaagcctcc attttccgtc gcctcagcgt ttacgataac
    56821 ctgatggcgg tactgcaaat tcgtgacgac ttgtctgctg aacaacgtga agaccgcgcg
    56881 aacgagctga tggaagagtt tcacattgag cacctgcgtg acagcatggg gcagtcactc
    56941 tccgggggtg aacgtcgccg tgtagaaatt gcccgcgcac tggctgcgaa tccgaaattt
    57001 attctactcg acgaaccgtt tgccggggtt gacccgatct cggttatcga cattaaacgc
    57061 atcattgaac acctgcgcga cagcggcctg ggcgtactga tcactgacca caacgtgcgt
    57121 gaaacactgg cggtttgtga acgcgcttat atcgtcagtc aggggcattt gattgcccac
    57181 ggcacgccta cagaaatctt acaagacgaa cacgttaagc gtgtatacct tggggaagac
    57241 ttcagactct gatagggtag aagtttgcga cgttttagca ggagagtacg attctgaaca
    57301 tgaagcaagg tttgcaactc aggcttagcc aacaactggc gatgacgcca cagcttcaac
    57361 aggcgattcg tctgttgcag ttgtcgacgc tggaacttca gcaggagcta cagcaggcgc
    57421 tggagagtaa tccgctgctt gagcaaatcg acactcatga agaaatcgac acccgcgaaa
    57481 cgcaagacag tgaaacgctg gacaccgccg acgcgctcga acaaaaagag atgccggaag
    57541 agctgccgct cgatgccagt tgggacacca tttacaccgc tggtacacca tccggcacca
    57601 gcggtgacta cattgacgat gagctgccgg tctatcaggg cgaaacgacg cagaccttgc
    57661 aggattacct gatgtggcag gtcgagctga caccgttttc cgacactgac cgcgctattg
    57721 ctacctctat cgtcgatgcc gttgatgaca ccggttatct gactgtcccg ctggaagata
    57781 ttctcgaaag tatgggcgat gaagagatcg acatcgacga ggttgaagcc gtccttaagc
    57841 ggatccaacg gtttgatccg gtcggtgtag cggcaaaaga tctgcgtgac tgcctgctaa
    57901 tccaactctc ccaattcgat aaaaccacgc cgtggctgga agaggccaga ctgatcatta
    57961 gcgatcatct cgatctgtta gccaatcacg acttccgcac tttaatgcgc gtcacgcgtc
    58021 tgaaagaaga tgtgctgaaa gaagccgtca atctgatcca gtcgctcgat ccgcgccccg
    58081 ggcagtcgat ccagactggc gaacctgagt atgtcattcc agatgtgctg gtgcgtaagc
    58141 ataacggtca ctggacggta gaactcaaca gtgacagcat tccgcgtctg caaatcaacc
    58201 agcactacgc ctcgatgtgc aataacgcgc gcaacgatgg tgacagccag tttatccgca
    58261 gcaatctgca ggatgccaaa tggttgatca agagtctgga aagccgtaac gatacgctac
    58321 tgcgcgtgag tcgctgtatc gttgaacagc agcaagcctt ctttgagcaa ggtgaagaat
    58381 atatgaaacc gatggtactg gccgatatcg cccaggctgt cgaaatgcat gaatcgacga
    58441 tatctcgcgt gaccacgcaa aaatacctgc atagtccacg aggcattttt gaactgaagt
    58501 atttcttttc cagtcacgtc aataccgagg gcggcggcga agcttcctcc acggcgattc
    58561 gtgcgctggt gaagaaatta atcgcggcgg aaaacccagc gaaaccgttg agcgacagca
    58621 agttaacctc tttgctgtcg gaacaaggta tcatggtggc acgccgcact gttgcgaagt
    58681 accgagagtc tttatccatt ccgccgtcaa accagcgtaa acagctcgtt tgacccaacc
    58741 gataaggaag acactatgca gctcaacatt accggaaata acgtcgagat caccgaggca
    58801 ctgcgcgaat ttgttacagc caaatttgcc aaacttgagc aatattttga ccgaatcaac
    58861 caggtctatg ttgttctgaa agtggagaaa gtcacccaca cctcagatgc aacactgcat
    58921 gtaaacggtg gtgaaattca tgccagcgcg gaaggtcagg acatgtacgc tgccattgat
    58981 ggcttaattg ataagctggc acgccagttg accaaacata aagataaact gaaacaacac
    59041 taattgtccg ggcaattagc atgtgcatgg cggcctgttg tgcggcacaa cgggccattt
    59101 gtacggttaa tgctccgagc ctgttccact gtttgagtgg gcaggttctt aggtgaaatt
    59161 atgacaaata atgatacaac tctacagctt agcagtgttc ttaacaggga atgtacgcga
    59221 agccgcgtcc actgtcagag caaaaaacgc gccctggaaa tcatcagcga gctggcggcg
    59281 aaacaactta gcctgccgcc tcaggtggtt tttgaagcta tcctgacgcg ggaaaaaatg
    59341 ggcagtaccg gtatcggcaa tggtattgcc attccgcatg gcaaactgga agaagatact
    59401 ctgcgcgccg ttggcgtttt cgttcagctc gaaacgccta tagctttcga tgccatcgac
    59461 aaccaaccgg tggacttact ttttgccctg ctggtgccgg cagaccaaac taaaacgcac
    59521 ctgcatactc tgtcgctggt ggcgaaacgt ctggcggaca aaaccatctg ccgccgttta
    59581 cgcgcagccc agagcgatga agagctgtat caaatcatta cggataccga aggtactccg
    59641 gatgaagcgt agttattcgg taatgtctct tttagacgtt gtgaggagaa acagtacatg
    59701 gtactgatga tcgtcagcgg acgttcaggt tcaggtaaat ctgtcgccct gcgtgcgctg
    59761 gaagatatgg gtttttactg cgtggataac cttcccgtag tgttgttacc cgatctggct
    59821 cgaaccctgg ccgatcgcga gatttctgcc gccgtcagca ttgatgttcg taatatgccg
    59881 gagtcaccag aaatattcga acaggcgatg agtaacctgc ctgacgcttt ctcaccgcaa
    59941 ctcctgttcc tggatgccga ccgtaatacc ttaattcgtc gttacagtga cacgcgccga
    60001 ctgcatccgc tttccagcaa aaacctgtcg ctggaaagtg ctatcgacaa agaaagcgat
    60061 ttgctggagc ctctgcgttc gcgagcggat ctgattgtcg atacctcaga aatgtccgtt
    60121 cacgagctgg ctgaaatgct gcgtacccgt ctgctgggta aacgtgaacg cgaactgacc
    60181 atggtctttg agtctttcgg cttcaaacac ggtatcccta tcgatgcaga ttacgtcttt
    60241 gacgtgcgct tcttgccgaa cccgcactgg gatccgaaac tgcgtccaat gacaggtctt
    60301 gataaacctg tcgccgcgtt cctcgaccgc cacacagaag tacacaattt tatctaccag
    60361 acgcgaagct atcttgagct atggttacct atgctggaaa ccaacaaccg tagctacttg
    60421 acggtcgcca ttggttgtac cggcgggaag caccgttcgg tgtatattgc agagcaactg
    60481 gcagactact tccgctcgcg cggtaaaaac gtccagtcac gccatcgtac gctggaaaaa
    60541 cgtaaaccat gaccgtcaag caaactgttg aaatcacaaa caagctgggc atgcatgccc
    60601 ggcctgcaat gaagctgttt gaattaatgc agggttttga cgctgaagtg ctgttacgta
    60661 atgacgaagg caccgaggcc gaagccaaca gcgttattgc gctgctgatg ttggattctg
    60721 ccaaaggacg gcagattgaa gttgaagcga ccggcccaca ggaagaggaa gcactggccg
    60781 ccgttatcgc cctctttaac tctggttttg atgaagatta atcttcatca ctttgacata
    60841 caactccctt caaactcccc ccgacaataa gaaaatcacg tactgaaatc gttctcaatc
    60901 aacgtcattt gtacattttg tgcgcttttc actttcagaa gaaccttaag aaaaccttaa
    60961 gaggcattgt ttaggttttg tttaagttaa tcgaccatac tggagatcgt cagaaaatat
    61021 ttccaggaga tggcatgatt cgcttatctg aacaaagtcc cctgggcacc ggaagacatc
    61081 ggaaatgtta tgcgcatccg gaagatgccc aacgctgtat caagattgtc taccatcgtg
    61141 gcgatggtgg cgataaagag atccgccgtg agttaaagta ctacgcgcat cttggtcgcc
    61201 ggttaaaaga ctggagtgga ataccgcgct atcacggtac cgttgagacg gattgcggaa
    61261 ccggttatgt ctacgatgtg attgcagatt ttgacggcaa accctccatt acgctgaccg
    61321 aatttgctga acaatgccgt tacgaagaag acattgcaca actgcgccag ctactgaaac
    61381 agctgaagcg ttatttgcag gataaccgta tcgtgacgat gtcgctgaag ccgcagaata
    61441 tcctctgtca ccgcatcagt gagtcagagg ttatcccggt cgtctgcgac aacattggtg
    61501 aaagcacatt gattccactg gcgacctggt cgaagtggtg ttgtctgcgt aagcaagagc
    61561 ggttatggaa acgatttatc gcccaaccgg cgctggctat tgccctgcag aaagatctgc
    61621 aacctcgtga gagcaaaacg ctggccctca cttcgcgcga agcttaatcc agctggtgtt
    61681 gttgcataaa cggctcaccg cctaactgat acatctgccg taaaatccac gcctgacggc
    61741 tacgcacgta gcccgatggc gaggagactt tgaaacgaag tggattaggt aatacagctg
    61801 ccagtaatgc agcttccgac cgggtaagtt tgctcgcggg tttgtggaaa taacgttgtg
    61861 ccgcagcttc gacgccaaac acaccgtcgc caaattcggc gatattcagg taaaccgtca
    61921 ggatacgctt tttgctccag actgtttcta tccccagcgt taatccggct tccagccctt
    61981 ttcgaaccca gctacgccca tcccataaaa agagattttt ggctgtctgt tgagaaatcg
    62041 ttgaagcacc gcgaatacgg ttttcattgc gctcgttgtg cgccagggct ttctcaatgg
    62101 aagcgacatc aaagccccag tgctcaggaa atttctgatc ttctgcggca atcaccgcca
    62161 gtcccatcca cggcgaaatt tgatccatac tgacccagtc agaatgtgcc acgtaacgaa
    62221 aattgccatg cagccaggcg ctgacctgtc gctcgaccat taccgcagag aagggaacag
    62281 gcgcaacgct aaacaacgcg atgcccccgc cccagaaaac ggcgagaaca accattaacc
    62341 gcaaaagaaa acggcgaacg aaactaaaca ccgttaagcg gcttttactc attcagccag
    62401 aaccagcacg cgggatacca gcttatcaat gccgctcgcc gcttctgcaa tgttctgcgc
    62461 cagcatatat gctggggtgg tgacaatctt attgtcttca tcaaccacga tatcatcgac
    62521 aggacacggc acatgctccg cgcccatctc ttccagcact tctgcggtat cgatatcagt
    62581 accgatggtc aaacgcagcg ggaaatcgaa aattttcggc agcatcgccg gggcaataca
    62641 cataaaacca agcggttttc cggcctgatg catcgcttgt gccagcgcct ttaattcacg
    62701 gtcaacggtg cattcgctac caagactggc aaaattgctt aaattcttcg ccgcgccaaa
    62761 cccccccggc acaatcaacg catccagttc agcggcatcg gcctgggcca gaggacggat
    62821 ttcaccgcgc gttattcgtg ccgcttcaat cagcacattg cgcgtttccg tcatcgcctc
    62881 gccagttaaa tggttgataa catcaacctg ctgcttatcc ggtgcaaagc agaccgcctg
    62941 cgcaccgctg cgtgaaatag ctaacaacgt caacaccgct tcatgaattt cagaaccgtc
    63001 atagacgccg catccgctca gaattacgcc aattttcttc attgtgatca tcctttcgca
    63061 attgactgaa acacattaat ttttttaata aaaatggtac gcatcacaca tttaactgat
    63121 tcatgtaaca aatcatttaa gttttgctat cttaactgcg tgcggcctga aaaacagtgc
    63181 tgtgcccttg taactcatca taataattta cggcgcagcc aagatttccc tggtgttggc
    63241 gcagtattcg cgcaccccgg tctagccggg gtcatttttt agtggctttt gccacccacg
    63301 ctttcagcac ttctacgtcg tgacgccact cttctttcat ctcttcaatc cattcaccga
    63361 cgttatcttc ccaagcagga aggtcaggag actgaatttg ctgacccagc tgttgcagat
    63421 ggcgtaaccc cactgacccc gccgcacctt taattttatg tccttcctca acaatgcctt
    63481 ttttatcctg cgccgtcaga ttcgactcca gcacgctgac atagcccggc atcatcttct
    63541 caaacaccgc taacccgtcg gtgatcagct tcggtcctac aagttcgaga tactgttcca
    63601 gcatgggaat atcgagcaat gcttctgatt tactgttctc ttctgtcgtc accgtactct
    63661 cctcatcatc ctgggtatcc cagaatttct tgatcatcgc ggttagcgcc ggaacagaaa
    63721 gcggcttact cagcacatca tccattccag cattgaggta ttcttgtttg tctttcagca
    63781 cgttagcggt taaggccacc agcggcggta aatcctctcg cggataacgc ttcgtcagtt
    63841 cacgagagat atccagcccg gtcatatctg gcaactgaat atccagtaac accaggtcgt
    63901 attcgcccgg tttaaacatc tctagcgccg ccttgccggt catggcgaca tcaacgctgt
    63961 tacctaattt ttccagcaca gaacgcgcga caatcacgtt cagttcaatg tcttccacca
    64021 gtagcacatt cagcgccggt aaaggcatat cgtcttcatc aaacgcatca tcgacctctt
    64081 ctgccaccga cggtgcgtgg atcgtcaacg taaaggttga acctttgccc tgttcgctgg
    64141 taaccgtaat atcgccgccc atatttttcg ccagacgacg agaaacggcc agaccaatac
    64201 cggtgccggt ggcaggttta ccgccatgac tgtctttcac ctggtaatac atggcgaaga
    64261 ttttatccag ctcatcctgc ggaatgccga taccagaatc ttccacttca aaatgcagca
    64321 tatcgccttc atcgtagcgc acgcgcacgg taacctggcc ttgctgggtg aatttgacgg
    64381 cgttactgat gaggttccac aggatctgcc gtaaacgcgt cccgtcggta atgacctgat
    64441 gcggtaatgg cagcgtaggc tccaggttaa agcgcaatcc tttttgttgc gcctgcaatg
    64501 cggagagatt ttccagatcg gcaaggaagc tggtgaaatc aaccggttga ttatcaagct
    64561 ggaccttgcg ccgttccatc ttatccatgt cgataatatc gttaaagata ttccccagcg
    64621 tgacggccga aacatggatg gtcttgagat atttttcctg ctcggcggtg agttcggtat
    64681 ccagcagaat gcggctcagg ccgacgatac cattcagcgg tgtacgcaat tcgtgactaa
    64741 tggtggagat aaacgtcgtt ttgtcgcggc tagcgcgttc aagcgcatcc tgataccgct
    64801 tacgctcggt aatgtcgcga ccaaagccca tcaatccgtg acgtttaccc acgcggtcgt
    64861 agtacggcac tttacggatt tcaaagcagg ctttgcgccc gtccgggtaa tccagccact
    64921 gttcataggt cagtgacaca ttatgacgga acactttttc atcggtttca atgacttttg
    64981 cggcggcttc cggtgagtaa acatcagcag gtttcaggtg aatcagttgt ttttcgcttt
    65041 ttccggtcag cagctccatc gcgcggttac agccggaaaa ctctttatct tcgttacgat
    65101 aaaaaaccag gtcgggtgaa gcatcaagga aggaacgtaa gaatgaggat tgctgctcga
    65161 gctgaatttg tgtctcttcg cgctctttga tttcaatttt cagttggccg aaggtttcct
    65221 gcagttctgc ttccgctttt tcacgaacgg caatttcctg atttagctgg gcaatattat
    65281 cttttaactg aacgttgagg ctcaaatcgc gctcgcgcat ctcctccagt ttttgcacca
    65341 gccgtgacag acgttgtcgt gactcctcca gttgctcgac gaccaccgat agaaagtaga
    65401 ccgcccacgg cgtaatcagc aaaccaaaga agatagaacg aataacatca atgctttcga
    65461 cctgaccatg cagcaccatg gttaccgcca tttgtaccac aatggcaaga acgacgaggg
    65521 ccagcgccag caacattgag aagcgcacca gacctaactt catcatcagg tcaacataat
    65581 actgcgccag cagacgaatt tgcttcatta gggaattcct tcacgacaac ctgccccaat
    65641 aatacccaat tctgacagtt acgttataaa ttgtgtgaga aatgcggaat tcccctcaac
    65701 gacctactcc gttggaggta gccacggacg tcccagcgcc gatccctgta cgccatgttc
    65761 attcagatag cgatccagct cgaccattcc cgtccatcga ttttcgcacc acagcggagc
    65821 aagcagcgtc gggcgacggg cgctggcgga aatgcggtgg tagatcactt ccggtggcgt
    65881 atggcgaatc atttctccgg cagtgagcgt gtaatcctcc agttcaatac cgttcaaacg
    65941 ccccgcttcc caggctttcg ccataatgct gcctttcaca atatgcagcg gatgcagctt
    66001 tatgccgtcc acgccggttt caaccactcg ctccagcgtt tgcaaacatt cagcctgccc
    66061 ttcgccaggc aggccgacaa ttaagtggga acacaccttc agcccgcgct gacgtgccag
    66121 ctgggttgta cgctgataac aggcaaaatc atggccgcgg ttgatgcgat gcagtgtttt
    66181 gtcgtgggcg gtttgtagcc ccagctccag ccacacttcg taaccctggt ccttatattc
    66241 gcaaagcaga tccagcaccg catccggcac gcagtccggg cgggtaccaa cacacaaacc
    66301 gacaatattg gcctggctca ccgcctgctg atacatagaa cgcagcacct gaacttccgc
    66361 aaaggtgctg gtatacgcct gaaagtaggc cagatagcgt ttagcgcggt taactaaatt
    66421 cgcctggtgc gccagttgct cggcaatgga acgatgctgc tgcgcttcat cggcaaacga
    66481 ggccacatta cagaatgtgc agccaccacg cccgatggta ccgtcacggt tagggcagct
    66541 aaaaccgccg tgcagcgtca gcttatgcac cttttgccca taacgacggg tgagatcacc
    66601 accaaacata ttgactaatt tctgtaactg cataatctga tagaccgcgc cttgaaaaga
    66661 ggccaaagcc tgccattttt agctcaattc ggcgatgacc tggatcaatc gtcccggcct
    66721 gcttttatca actgcataat caatcagaat taccgaaatt tcatgcataa tcacataaat
    66781 cacttttgct tatcttgtgt cagatttttt tatctcctta tggattttag gcaaaaacag
    66841 tagcatgaaa cgtcattacc aattaaggca gtataaaatg ctggttttct cgtcagttca
    66901 aggcaggata agggttaaca cgcctttatg acagtcagga attgactgtt tctctaacga
    66961 cttccctttt agccttaaag ataaaatcta ttttaatttc agccatttaa taaagaattt
    67021 tgcgctaaag cacatttctg taccaataag cttgccattt gacctgtatc agctttcccg
    67081 ataagttgga aatccgctgg aagctttctg gatgagcagc ctgctcatca tatttatgca
    67141 gtaattgaga tcccctcttc accgtattaa ccgatgcgaa aaggacaaca agggggcgaa
    67201 tgcgaggcgc gcgtatgaca cgcaaccccc gtcgccacgc tctttctgtg cccgtgcgca
    67261 gcggttcgga agtggggttc ccgcagagcc tgggggaggt tcacgatatg ttgtacgata
    67321 aatcccttga gagggataac tgtggtttcg gcctgatcgc ccacatagaa ggcgaaccta
    67381 gccacaaggt agtgcgtact gcaatacacg cactggcccg catgcagcac cgtggcgcga
    67441 ttctcgccga tggtaaaacc ggcgacggtt gcggcttgct gttacaaaaa ccggatcgct
    67501 tttttcgcat cgttgcgcag gagcgcggct ggcgtttagc gaaaaactac gccgtcggga
    67561 tgctattcct gaataaagat cctgaactcg ccgctgccgc acgccgcatc gttgaagaag
    67621 agctacaacg cgaaaccttg tcgattgtgg gctggcgtga tgtccccact aacgaaggcg
    67681 tgctgggtga aatcgccctc tcctctctgc cacgcattga gcaaattttt gtgaacgccc
    67741 cggcaggctg gcgtccacgc gatatggagc gccgtctgtt tatcgcccgc cgccgcattg
    67801 aaaagcgtct cgaagccgac aaagacttct acgtctgtag cctgtcgaat ctggtgaaca
    67861 tctataaagg tctgtgtatg ccggcggatc tgccgcgctt ttatctggat cttgcggacc
    67921 tgcgtctgga atcggccatt tgcctgttcc accagcgctt ctccactaac accgtaccgc
    67981 gctggccgct ggcgcaaccg ttccgctatc tggcgcataa cggtgaaatc aacaccatca
    68041 ctggtaaccg ccaatgggcg cgtgcgcgta cctataaatt ccagacgccg cttatccctg
    68101 atctgcacga cgccgcaccg ttcgtcaacg aaaccggctc tgactccagt tcgatggata
    68161 acatgctgga actgctgctg gcaggcggga tggatatcat ccgcgccatg cgtctgttag
    68221 tgccacccgc ctggcagaac aacccggata tggacccgga actgcgtgcc ttctttgact
    68281 ttaactccat gcatatggag ccgtgggatg gcccggcggg catcgtgatg tccgacggtc
    68341 gttttgccgc ctgtaacctc gaccgtaacg gtctgcgtcc ggcgcgctac gtcatcacca
    68401 aagataagct catcacctgc gcctctgaag tcggtatctg ggattaccag cctgacgaag
    68461 tggtcgaaaa aggccgcgtc gggccaggcg aactgatggt tatcgacacc cgcagtgggc
    68521 gtattctgca ctcggcagaa accgatgacg atctgaaaag ccgccatcca tataaagagt
    68581 ggatggagaa aaacgtccgc cgactggtac cgtttgaaga tctgcccgat gaagaagtgg
    68641 gtagccgcga actggacgac gacacgcttg ccagctacca gaaacagttt aactacagcg
    68701 cggaagagct ggactccgta attcgcgtac tgggcgaaaa cggtcaggaa gcggtcggtt
    68761 cgatgggcga tgatacccca ttcgccgtgc tctccagtca gccgcgcatt atttacgact
    68821 acttccgcca gcagtttgcc caggtgacta acccgccaat cgacccgctg cgtgaagcgc
    68881 atgttatgtc gctcgccacc agtatcggtc gtgaaatgaa cgtcttttgc gaagcagagg
    68941 gccaggcgca ccgtttaagc tttaaatcgc cgattctgct ctactccgat ttcaaacagc
    69001 tcacgacgat gaaagaggag cactaccgcg cagatacgct ggatatcacc tttgacgtca
    69061 ctaaaaccac gctcgaagcg acagtcaaag agctgtgcga caaagccgaa aaaatggtac
    69121 gtagcggcac cgtgctgctg gtgctctccg accggaatat cgctaaagat cgcctgccgg
    69181 ttccagcccc gatggcggtt ggcgcgatcc agacccgtct ggtcgatcaa agcctgcgtt
    69241 gcgatgccaa catcatcgtc gaaaccgcca gcgcccgcga tccgcaccac ttcgccgtgt
    69301 tgctgggctt cggcgcgacg gctatttatc cataccttgc ctatgaaacg ctgggccgcc
    69361 tggtagacac ccatgcgatt gccaaagatt atcgtaccgt gatgctcaac taccgtaacg
    69421 gcatcaacaa aggcttgtac aaaatcatgt ccaaaatggg catctccacc atcgcctctt
    69481 accgctgctc gaaactgttt gaagcggtcg gtctacacga tgatgtagtg ggcctgtgct
    69541 tccagggtgc ggtcagccgc attggggggg cgagctttga agacttccag caggatctgc
    69601 tgaatctgtc gaaacgtgcc tggctggcgc gcaagcccat cagccagggc ggcctgctga
    69661 aatacgtcca cggcggcgaa taccacgcct acaacccgga cgtggtgcgc acgctgcaac
    69721 aggcggtaca gagcggcgag tatagcgact atcaggaata cgcgaagctg gttaatgagc
    69781 gtccggcaac cacgctgcgc gatctgctgg caattacgcc gggtgaaaac gcggtcaaca
    69841 ttgctgatgt tgaaccggca agcgaactgt ttaaacgctt cgataccgct gcgatgtcta
    69901 tcggcgcgtt aagcccagag gcccacgagg cgctggcgga agcgatgaac agcatcggcg
    69961 gtaattcgaa ctccggtgaa ggcggcgaag acccggcgcg ctacggcacc aacaaagtgt
    70021 cgcgcatcaa gcaggtggct tccggtcgct ttggggttac tccggcgtat ctggtcaatg
    70081 ccgacgtcat tcagattaaa gtcgcccagg gcgcgaagcc gggcgaaggc ggtcagttgc
    70141 cgggtgataa agtcacgcct tacatcgcca aactgcgcta ttcggtgccc ggagtgacgc
    70201 tgatctcccc gccgccgcac cacgatatct actctatcga ggacttagcg cagctcattt
    70261 tcgacctcaa gcaggttaac ccgaaagcga tgatctccgt gaagctggtt tccgaaccgg
    70321 gagtaggcac catcgcgact ggcgtggcaa aagcttatgc ggacttgatc accatcgcag
    70381 gctatgacgg cggcaccggc gcaagtccgc tttcatcggt gaaatacgca ggctgtccgt
    70441 gggagctggg gcttgttgaa acccagcagg cgctggttgc taacggcttg cgtcacaaga
    70501 tccgtttgca ggtcgatggc ggcctgaaaa cgggtgtcga tatcatcaag gcggcgattc
    70561 tcggcgcaga aagcttcggc ttcggcactg gcccgatggt ggcgctcggc tgtaaatatc
    70621 tacgtatttg ccatctgaac aactgcgcaa cgggtgtagc aactcaggat gacaaactgc
    70681 gtaagaacca ctatcacggc ctgccgttca aggttaccaa ttactttgaa tttattgccc
    70741 gtgaaacccg cgagctgatg gcacagctgg gcgtaacgcg tctggtggat ctgattggtc
    70801 gcaccgacct gctgaaagaa ctggacggtt tcaccgccaa acagcaaaaa ctggcgctgt
    70861 cgaagctgct ggagaccgcc gaaccgcatc caggtaaggc actctactgc accgaaaaca
    70921 acccgccgtt tgataacggc ctgctgaacg cgcagttgct gcaacaggcg aaaccgtttg
    70981 tcgatgagcg ccagagcaaa accttctggt tcgatattcg caacaccgac cgttctgtcg
    71041 gcgcgtcgct ttcaggctat atcgcccaga cgcacggcga tcaggggctg gcagccgatc
    71101 ctatcaaagc gtacttcaac ggcaccgcag gccagagctt cggcgtgtgg aacgcgggcg
    71161 gcgtggaact gtacctgacc ggtgatgcca acgactatgt cggtaaaggc atggcgggcg
    71221 gcttaatcgc cattcgtcct ccggttggtt ccgccttccg cagccatgaa gcaagcatta
    71281 tcggcaacac ctgcctgtat ggcgcgaccg gtggtcgtct gtatgccgca ggccgcgcgg
    71341 gtgaacgttt cggcgtgcgt aactccggtg ctatcaccgt ggtagaaggc attggcgaca
    71401 acggttgtga atatatgacg ggtggtatcg tctgcattct gggtaaaacc ggcgttaact
    71461 tcggtgcggg catgaccggc ggtttcgctt acgttctcga tgaaagcggc gatttccgca
    71521 aacgcgttaa cccggaactg gtcgaggtct taagcgttga cgctctggcg atccatgaag
    71581 agcatctgcg tggtcttatc accgagcatg tgcagcatac cggctctcag cgcggtgaag
    71641 agattctggc gaactggtca accttcgcca ctaaatttgc gctggttaaa ccgaagtcca
    71701 gtgatgtaaa agcactgctg ggtcaccgta gtcgtagcgc agctgagttg cgcgtgcagg
    71761 cgcagtaagg ggtagcaaca atgagtcaga atgtttatca atttatcgac ctgcagcgcg
    71821 ttgatccgcc aaagaaaccg ctgaagatcc gcaaaattga gtttgttgaa atttacgagc
    71881 cgttttccga aggccaggcc aaagcgcagg ctgaccgctg cctgtcgtgc ggcaacccat
    71941 actgcgagtg gaaatgcccg gtacacaact acatcccgaa ctggctgaag ctcgccaacg
    72001 aggggcgtat ttttgaagcg gcggaactgt cgcaccagac caacaccctg ccggaagttt
    72061 gcggacgagt ctgcccgcaa gaccgtctgt gcgaaggttc ctgcactctg aacgatgagt
    72121 ttggcgcggt gaccatcggc aacattgagc gttatatcaa tgataaagcg ttcgaaatgg
    72181 gctggcgtcc ggatatgtcc ggcgtgaagc aaaccggtaa aaaagtggcg attatcggcg
    72241 caggcccggc aggtctggcg tgtgcggatg tcctgacgcg taacggcgta aaagccgttg
    72301 tcttcgaccg tcatccagaa attggcggac tgctgacctt cggtattccg gccttcaagc
    72361 tggaaaaaga ggtaatgacg cgtcgccgtg aaatcttcac cggcatgggt attgaattca
    72421 aactcaatac cgaagtgggc cgcgacgtgc agctggacga tctgctgagt gattacgatg
    72481 ccgtcttcct tggcgtcggg acttatcagt caatgcacgg cgggctggaa aacgaagacg
    72541 ccgatggcgt gtacgcagcg ctgccgttcc ttatcgccaa caccaaacag ttaatgggct
    72601 ttggtgaaac ccgcgacgaa ccgttcgtca gcatggaagg caaacgcgtg gtggtccttg
    72661 gcggtggcga cactgcgatg gactgcgtgc gtacgtccgt gcgccaggga gcgaagcacg
    72721 ttacctgtgc ctatcgtcgt gatgaagaga acatgccggg ttcccgccgc gaagtgaaaa
    72781 acgcgcggga agaaggcgta gagttcaaat tcaacgtcca gccactgggg attgaagtga
    72841 acggtaacgg caaagtcagc ggcgtaaaaa tggtacgtac cgaaatgggc gaaccggatg
    72901 ccaaaggccg tcgccgcgcg gagatcgttg caggttccga acatatcgtt ccggcagatg
    72961 cggtgatcat ggcgtttggt ttccgtccac acaacatgga atggctggca aaacacagcg
    73021 tcgagctgga ttcacaaggc cacatcatcg ccccggaagg cagcgacaac gccttccaga
    73081 ccagcaaccc gaaaatcttt gctggcggcg atatcgtccg tggttccgat ctggtggtaa
    73141 ccgctattgc cgaaggtcgt aaggcggcag acggtattat gaactggctg gaagtttaag
    73201 ctctgtccca gcgccgcctc ctcagaaaag agtcggcgct gaaattctcc tttctttctg
    73261 gaaagcgcct cgcataatct gaagaaaacc aatgtaatac gctcatctgg attgttaccg
    73321 gtagatgccc ggcgtatagc tgattccatt attcaacgca gcgccatata aagggacaga
    73381 taatggaatc gctctcagag agaacctcaa caggctacca gcaaatccat gacggtatta
    73441 ttcacctggt cgatagcgcc cggacggaaa cggtacgtag cgttaacgcg ctgatgaccg
    73501 cgacatactg ggaaattggc cgacgaattg tcgaatttga acaaggtggc gaggccaggg
    73561 ctgcgtatgg tgcgcagcta atcaagcgac tatcaaagga tttaagtcta aggtataagc
    73621 gtggtttctc tgcaaaaaac ttacgccaaa tgaggctttt ttacctcttt tttcaacatg
    73681 ttgaaattcg ccagacagtg tctggcgaat taacaccatt gggaattccg cagacaccat
    73741 ctgcagaatt cccatcggca aagatttggc agacgctgtc tgccaaattg agaggctgtt
    73801 tgatatttcc acagcttttg tattgcatct cctattttcg gtttgtatat tcatgcaaat
    73861 aaacgaagga aagttgccat gatttacatt gaacagcttg aattaatcca taaaagtggt
    73921 gatgtgttgt atccagtaaa aataaccaga aaatcatcag gaaaaactgc cttccacctt
    73981 gttccattcg gcttaaataa gactcacgac cttctagagg tggaagatgc ttcagaagca
    74041 attcggctgg tgattgatga acgtcattcc attcgctgca gtacacttac agcaactatt
    74101 acgaataaaa agggcaagcg aattaagcgg acaggtatat acagtatcaa gggtgttaac
    74161 ataaaagaat acaacgtcag gtaatggcac agagcggtcc gtcagttcag gtttggctct
    74221 gtgccaaaga tgggtcaggt cacacctgat ctaataaatt taagccatta aatcagcctt
    74281 aaccttcacc accacctttt taatctcacc cggctcgcca acaacgcatc ccggcttatg
    74341 cggctctcct ggcataaaca cggcgaacat tcccggtttc aggatgatgg tttgctcgtt
    74401 ttcaatggcg ctacaaagct ggtaatcatc ttcatggtgg aattcttcac attggcgcgc
    74461 agtgcctgcc atgccaaaca gaatccgttc ctcaccgttt aataacagct ggatatcaat
    74521 gtattgctcg tgcaattccg cttttttctc gacgggcgat tgagtgttaa acgtcatgac
    74581 attcataaaa atattgtcgc cctgtaattc gtaacgaccc ggcgcttttt cttgcggtct
    74641 ggcagctaag gccagcgtta acgcgtcctg taacgcagga tgtaacccag cagacggtaa
    74701 tgactgtact tcacccatca tcataatttt tctccctggg ccaacagcgc agccccaagt
    74761 aaacctgcat catggcggta atgcgccgcc agtaaatcaa catgaaatgc cgctggctcc
    74821 tgcgccagat acatttccac caacgccaga tacccttctg ccagaccaac gctgccacca
    74881 accaccacgc actggcaatc agttgtggct ttaatatcag cgatcagcct tgcaagcgta
    74941 cgtgcggagc gatgaatcag ttgctgcgcc tgctcatcgc cctgcccggc gcgtgtgaaa
    75001 atagttctcg catccgcgcc agccagctcg ccctgcgctg ccgccgcaat gccgcgacca
    75061 gaagcaattg cttccacgca acctgtgcgt ccacagccgc agactgggcc gtgtggatcg
    75121 gcaagcgtat gcccgatgtg ccccgccaga ccgccagggc cggtaagcag tttgccgccg
    75181 ctcactacac cgccgccgac gccagtggaa acggtgataa agaccatatc ggttatatcg
    75241 ccttccagcg cctgatactc tgcccatgct gcggcctgcg cgtcgttaat ggcaatggtc
    75301 ggcaaattgg taagttgttc cagcgttttg actaacggaa agtgtagcaa tccaccgaga
    75361 ttatgcggat tcagcgccag caaactgccg tcgcgaataa ttccggttga agcgatggca
    75421 acccgctgcg catgagcttg caacggagag actaatgcgg ataaggcatc acgcaaggct
    75481 tctggtgtct ggctggctgg cgtaggaagt tcacgacgat cgcggatctg cccgtcagcg
    75541 ccaatcagcg cggcggcaag tttagtaccg ccgatatcaa tcgccagtgt ggtcatagca
    75601 ccgccttttt catcgctgtg ttgtaccact gacaaatgtg ttcaagacgc gtaatggcag
    75661 aaccgaccgt caccgcccac gcgccgtggc gcattgcgtc tgccgcctga gcaggcgtgt
    75721 tgtaacgccc ttcggcaatc acccgacatc cggcgtcgct caacgttttc accagcgcca
    75781 gatccggctc ttctggcgtt tcaggcgtgg tatagccaga aagcgtagtg ccaataattt
    75841 cggctcccag cttttggcat gccaggccgt cttccggcgt tgagcagtcg gtcatcgcca
    75901 gtaaaccgtg atggtgaata cgtgccagca gcgtttcaac aggcaccgga cgcgggcggt
    75961 cggtgccgtc aatggcgata atgtccgcgc ccgcctgcgc cagcgcatcg acatcttcaa
    76021 tataggccgt gatgcgtacc ggagaatcct ccagatcgcg tttcacaatc ccgataattg
    76081 gcacgctcac caccgcacgc gtggcttgca gatttgccac accttcaatg cgaatggcaa
    76141 ccgcgcccgc ctgctctgcc gctaatgcca tggcggcgac gatttcgggt ttatcgagcg
    76201 gactgtccgg aaccggctgg caggagacaa tcaggccacc gttagcagcg attttttgat
    76261 ccagttgtgc aagtaacgac atacatcttc ccttagcgaa aggcccggta cagagaccgg
    76321 gcaacaggat taacttttgg ttttgactaa atcgtttttg gcgctgccaa atggcacggc
    76381 accgctaaat gatttgccgt cgatagcgtc atgagtacgc aatgcttccg ggcgcagcca
    76441 acgctgaacg cgggaaggca tatccagacc aatcagcagg atcaccacaa atgtcagact
    76501 gaacgagagc gatgccagcg cagtacccag atccagacgt tgagcgatca acgcgccgat
    76561 gattggggcc agtgcgccgc ccaatgcgcc aacgttgtag gtaaagccca ggcccgctgc
    76621 acgctggtcg gtatcgaaat aaccgccaat cagtttcggt aagatcccgg cgatcccttg
    76681 tccaagcatt tgctggaaga acagtaacag accgagcacc cagacgtttg cgccgccaat
    76741 cgcaaatacc ggaataatca gcagctgcga ggccagcagg ctacaaacgt acgctttgcg
    76801 ggttcccagc cagtcaccga ggaagccacc tacgcagcat cccacggccg cgccaaagcc
    76861 actaaagaac agcacattgg cgacagtatg cgggttataa gccagatcgg ttttcagata
    76921 cgttggcagc agcgcctgaa tcggccatga gtagaggaaa gcaaacaaca cgaccaccat
    76981 cagcattacg cccgttggcc agcgtttacc cgtactttgc accataaagc tgataaagat
    77041 tgcggcgcat aacagcccga gaacagcgac gattgcagca ttttgcaggt taccggcgaa
    77101 gcagaaccac agcgcagtag ccgccgccag tgtcattacg atattggcaa tgcgatgttc
    77161 accacggtag agaatatcca ccattgtgcg tactggtgct ttacctgcgt gtttctcttt
    77221 ccagtcttcc gcttccggga tgtttttacg cagccagaga gcaaagatga ttggcaaaat
    77281 gccgataaag aacagcgcac gccaacccca gaccggaacc accaggctat agacctgagc
    77341 ggcaacgacg gcccccacag agaagcctga aatcaaaaaa ccactggctt tgttacgcag
    77401 atgttttggc cagctttcaa tgacataggt ggcgctggaa ccgtattcac ccgccatccc
    77461 catgccgatg accagacgag cgataaacat ggtgatgtag cctggcgcaa agccgcaggc
    77521 cagcgtcccg gccgagaaga gaacgatgct ggtgaccatt gccagacgac gcccgtagcg
    77581 gtcacccata gcgccgagca tcaggccgcc gaaccagcga gagataaagg ctgcggagat
    77641 cagacttgcc gcctgcaccg tcgtcagccc gaattcaccc tgtacttcgg tgagtaccag
    77701 ggcgattaaa acgaaatcaa aaccgtcaag cagatatccc aaccaggcag cggaaaatgc
    77761 gcgccattga gcacggttga gatggcgata ccacgggata ttctgggttg tagtactcat
    77821 tgtgagtctc ccgcggtggg cgatgcccac acgctttggt atgaaaattg tagggtacag
    77881 atgcgtttat ttcccctcac ccggtagggg cgagcgaggg gaaaacaact cacccgcgct
    77941 cttgcatcaa ctgctgggcc agcgccttca gttctggcag atatttttca tctaccggtc
    78001 caaacggttt gcggcacagc ggcacagaaa cgacatccat ataatgcagg acagttttca
    78061 ggccgcggaa tacgcccgtt ttgatcagta aatcaatgac tttgttgcac tcagtttgca
    78121 gtttctgcgc ggtctggata tcgccttctt tcagcgcctt cacgatcccc tgatatcgcc
    78181 agcccatgat gttgtaagta ctgccgatac caccatcagc gcctgccagc aagccggaag
    78241 cgaagatttc gtcgtaaccg ttatagagca ccagatcagg atgttcacga cggatctgtt
    78301 ccatctgata gagatcgcca gaagtctgtt tcagcgcacc gacgccaggc aacgtaacca
    78361 gggtgttgat ctgatccagg gtcagtttta ccccactcag ggctggaatg ttgtacacca
    78421 ccatcggcaa accatccgct gaatcaataa ttgctcgata gtgatcgcag tgttcttcaa
    78481 agctgaaagg atagtagaac ggcgtgacgg cggagacggc atcgaagcca taacgtttag
    78541 ccgatgccgc aagttgttgg ctttcggcgg tgctgacgca accgacgtgg gcgatgagtt
    78601 taatcttacc tttcgcctct tcggcgacga tttccagtac ctgttcacgc tcggaaaggc
    78661 tttgtacaaa ggcttcgccg gtcgaaccac ccacgtataa accgtcgatg ccctgctgaa
    78721 tattgaactg aaccaggcgg cgcagactcg ctttatccag tgcttgttgt tgatcaaaag
    78781 gagtcaggag tgcagccatt acgccacgta aattcgttgc cataaatacc tctgaagtga
    78841 tgcttgtctg ataaacgata tacctttata cctgttatac cagatcaatt aagcaacacc
    78901 ccatacagaa agcttataat gcgatctgct tcactaaagt ggcattattt ctttttgttg
    78961 gtggcctgac cgaaggcgtg ccaggtagca gagacgctgt tgagatgcga ttgcaacgca
    79021 cgatcggctt cgtcaggatc atgacggcgg atcgcatcaa cgatcgcaat atgctgttga
    79081 tagctaacgt tgttatgctc gtgcagagcc tgatcagcca cggttggacg tgcggcgatc
    79141 agccagtcga gcagggcaac gtggatcgcc atgaagattg ggttacctgg gatctccgcc
    79201 agcacgcggt ggaaatcaac gtctgagcga atgaatgccg cgttgttatc cagcgactga
    79261 ctgttgattt ccagtgcttt tgccagcaaa tcgatttgct catcggtggc atgttcagcc
    79321 gcatagcgca ccaggctgga ttcaaagaac agacgtaact gttcgaaatg ggctatccca
    79381 ccaggatgag aaaggaaatc tttcgccatg ccggaaagct caccgatgat agtgtccgca
    79441 gaaggacgcg agacgcgagc gcgttcgccg ttgtttattt gcaccagacc tttgcgtttt
    79501 aacgctgcca gcgcttcacg caccgaagga cgcccgacgt taaagaacgc catcagttcg
    79561 cgttcagacg gtaattgttc accttcgcca aattcacgac ggcggatcat ctgttccagc
    79621 tcttcttcca ccatttcgga gagtttttta cgcgccagcg ggcggctacg caagttgcga
    79681 ccaattgcag gtgaagaatc ttcggtttgc gaatcaaatg cgttcataag gcccattctg
    79741 taaggtcagt gtgattaaca tcatcagtga catcctatca caggattgaa agtaggggaa
    79801 aatggcaggg ttttctcttt gtgcctcatc attaccataa ttaacggaat aattaactat
    79861 tgcgaaaaat taatgtaacg cagataaaaa catcccgttt gaattattta taagactatt
    79921 cacgag
//
LOCUS       DAOFQD010000006        73175 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.006, whole genome shotgun sequence.
ACCESSION   DAOFQD010000006 DAOFQD010000000
VERSION     DAOFQD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.006"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            52..495
                     /gene="cmtB"
                     /locus_tag="QBF46_000394"
     CDS             52..495
                     /gene="cmtB"
                     /locus_tag="QBF46_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDQ6672632.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLILLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            523..1911
                     /gene="cmtA"
                     /locus_tag="QBF46_000395"
     CDS             523..1911
                     /gene="cmtA"
                     /locus_tag="QBF46_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDQ6672633.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1926..3203
                     /locus_tag="QBF46_000396"
     CDS             1926..3203
                     /locus_tag="QBF46_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDQ6672634.1"
                     /translation="MKTKVAAIYGKRDVRLRVFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGKL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3200..4165
                     /gene="yggF"
                     /locus_tag="QBF46_000397"
     CDS             3200..4165
                     /gene="yggF"
                     /locus_tag="QBF46_000397"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDQ6672635.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISTC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4187..4696
                     /gene="fumE"
                     /locus_tag="QBF46_000398"
     CDS             4187..4696
                     /gene="fumE"
                     /locus_tag="QBF46_000398"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDQ6672636.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHTILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4693..5406
                     /locus_tag="QBF46_000399"
     CDS             4693..5406
                     /locus_tag="QBF46_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDQ6672637.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLTENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WLELASFCDFSIFIHAPAQILRERLISRKIAGGLTRQVAEAFYARTDGPNVERVLMNS
                     RQANLIVEMTEEGRYHFTS"
     gene            5691..6710
                     /gene="epd"
                     /locus_tag="QBF46_000400"
     CDS             5691..6710
                     /gene="epd"
                     /locus_tag="QBF46_000400"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDQ6672638.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6760..7923
                     /gene="pgk"
                     /locus_tag="QBF46_000401"
     CDS             6760..7923
                     /gene="pgk"
                     /locus_tag="QBF46_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDQ6672639.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8138..9217
                     /gene="fbaA"
                     /locus_tag="QBF46_000402"
     CDS             8138..9217
                     /gene="fbaA"
                     /locus_tag="QBF46_000402"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDQ6672640.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            9575..10435
                     /gene="mscS"
                     /locus_tag="QBF46_000403"
     CDS             9575..10435
                     /gene="mscS"
                     /locus_tag="QBF46_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDQ6672641.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            10574..11209
                     /gene="argO"
                     /locus_tag="QBF46_000404"
     CDS             10574..11209
                     /gene="argO"
                     /locus_tag="QBF46_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDQ6672642.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIIIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11302..12042
                     /locus_tag="QBF46_000405"
     CDS             11302..12042
                     /locus_tag="QBF46_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDQ6672643.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12209..13105
                     /locus_tag="QBF46_000406"
     CDS             12209..13105
                     /locus_tag="QBF46_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6672644.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTTQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(13102..14580)
                     /gene="scpC"
                     /locus_tag="QBF46_000407"
     CDS             complement(13102..14580)
                     /gene="scpC"
                     /locus_tag="QBF46_000407"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDQ6672645.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIAHR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(14604..15389)
                     /gene="scpB"
                     /locus_tag="QBF46_000408"
     CDS             complement(14604..15389)
                     /gene="scpB"
                     /locus_tag="QBF46_000408"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDQ6672646.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(15400..16395)
                     /gene="meaB"
                     /locus_tag="QBF46_000409"
                     /pseudo
     CDS             complement(15400..16395)
                     /gene="meaB"
                     /locus_tag="QBF46_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
     gene            complement(16388..18532)
                     /gene="scpA"
                     /locus_tag="QBF46_000410"
                     /pseudo
     CDS             complement(16388..18532)
                     /gene="scpA"
                     /locus_tag="QBF46_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(18736..19629)
                     /gene="argP"
                     /locus_tag="QBF46_000411"
     CDS             complement(18736..19629)
                     /gene="argP"
                     /locus_tag="QBF46_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDQ6672647.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20012..20671
                     /gene="rpiA"
                     /locus_tag="QBF46_000412"
     CDS             20012..20671
                     /gene="rpiA"
                     /locus_tag="QBF46_000412"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDQ6672648.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20927..22159
                     /gene="serA"
                     /locus_tag="QBF46_000413"
     CDS             20927..22159
                     /gene="serA"
                     /locus_tag="QBF46_000413"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDQ6672649.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22388..22447
                     /gene="ibsC"
                     /locus_tag="QBF46_000414"
     CDS             22388..22447
                     /gene="ibsC"
                     /locus_tag="QBF46_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDQ6672650.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(22548..23150)
                     /locus_tag="QBF46_000415"
     CDS             complement(22548..23150)
                     /locus_tag="QBF46_000415"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDQ6672651.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(23171..23354)
                     /gene="ssrS"
                     /locus_tag="QBF46_000416"
     ncRNA           complement(23171..23354)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="QBF46_000416"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23396..23725)
                     /gene="zapA"
                     /locus_tag="QBF46_000417"
     CDS             complement(23396..23725)
                     /gene="zapA"
                     /locus_tag="QBF46_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDQ6672652.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            23893..24471
                     /gene="ygfB"
                     /locus_tag="QBF46_000418"
     CDS             23893..24471
                     /gene="ygfB"
                     /locus_tag="QBF46_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDQ6672653.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24497..25822
                     /gene="pepP"
                     /locus_tag="QBF46_000419"
     CDS             24497..25822
                     /gene="pepP"
                     /locus_tag="QBF46_000419"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDQ6672654.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYCGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            25819..26997
                     /gene="ubiH"
                     /locus_tag="QBF46_000420"
                     /gene_synonym="visB"
     CDS             25819..26997
                     /gene="ubiH"
                     /locus_tag="QBF46_000420"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDQ6672655.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKVPGVTLHCPDRVANVARTQSQVEVTLESGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVMDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            27020..28222
                     /gene="ubiI"
                     /locus_tag="QBF46_000421"
                     /gene_synonym="visC"
     CDS             27020..28222
                     /gene="ubiI"
                     /locus_tag="QBF46_000421"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDQ6672656.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(28296..28379)
                     /locus_tag="QBF46_000422"
     CDS             complement(28296..28379)
                     /locus_tag="QBF46_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672657.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            28669..29763
                     /gene="gcvT"
                     /locus_tag="QBF46_000423"
     CDS             28669..29763
                     /gene="gcvT"
                     /locus_tag="QBF46_000423"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDQ6672658.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            29787..30176
                     /gene="gcvH"
                     /locus_tag="QBF46_000424"
     CDS             29787..30176
                     /gene="gcvH"
                     /locus_tag="QBF46_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDQ6672659.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            30295..33168
                     /gene="gcvP"
                     /locus_tag="QBF46_000425"
     CDS             30295..33168
                     /gene="gcvP"
                     /locus_tag="QBF46_000425"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDQ6672660.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            33435..34177
                     /locus_tag="QBF46_000426"
                     /pseudo
     CDS             33435..34177
                     /locus_tag="QBF46_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(34239..35672)
                     /gene="bglA"
                     /locus_tag="QBF46_000427"
     CDS             complement(34239..35672)
                     /gene="bglA"
                     /locus_tag="QBF46_000427"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDQ6672661.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFDWYKEVIASNGENL"
     gene            35717..36028
                     /locus_tag="QBF46_000428"
     CDS             35717..36028
                     /locus_tag="QBF46_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDQ6672662.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            36192..36851
                     /locus_tag="QBF46_000429"
     CDS             36192..36851
                     /locus_tag="QBF46_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDQ6672663.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(37047..38027)
                     /gene="ygfZ"
                     /locus_tag="QBF46_000430"
     CDS             complement(37047..38027)
                     /gene="ygfZ"
                     /locus_tag="QBF46_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDQ6672664.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSVLPSKEKQVIREDATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRLRDDANTLHIEPLPYSLEE"
     gene            38270..38536
                     /gene="sdhE"
                     /locus_tag="QBF46_000431"
     CDS             38270..38536
                     /gene="sdhE"
                     /locus_tag="QBF46_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDQ6672665.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            38517..38924
                     /locus_tag="QBF46_000432"
     CDS             38517..38924
                     /locus_tag="QBF46_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDQ6672666.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(38964..39485)
                     /gene="fldB"
                     /locus_tag="QBF46_000433"
     CDS             complement(38964..39485)
                     /gene="fldB"
                     /locus_tag="QBF46_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDQ6672667.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     ILHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            39597..40493
                     /gene="xerD"
                     /locus_tag="QBF46_000434"
     CDS             39597..40493
                     /gene="xerD"
                     /locus_tag="QBF46_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDQ6672668.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            40518..41228
                     /gene="dsbC"
                     /locus_tag="QBF46_000435"
     CDS             40518..41228
                     /gene="dsbC"
                     /locus_tag="QBF46_000435"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDQ6672669.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            41234..42967
                     /gene="recJ"
                     /locus_tag="QBF46_000436"
     CDS             41234..42967
                     /gene="recJ"
                     /locus_tag="QBF46_000436"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDQ6672670.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFKLFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            43058..44156
                     /gene="prfB"
                     /locus_tag="QBF46_000437"
     CDS             join(43058..43132,43134..44156)
                     /gene="prfB"
                     /locus_tag="QBF46_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDQ6672671.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            44166..45683
                     /gene="lysS"
                     /locus_tag="QBF46_000438"
     CDS             44166..45683
                     /gene="lysS"
                     /locus_tag="QBF46_000438"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDQ6672672.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQNILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(45726..46274)
                     /gene="idi"
                     /locus_tag="QBF46_000439"
     CDS             complement(45726..46274)
                     /gene="idi"
                     /locus_tag="QBF46_000439"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDQ6672673.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            46329..46400
                     /locus_tag="QBF46_000440"
     CDS             46329..46400
                     /locus_tag="QBF46_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672674.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(46397..46522)
                     /locus_tag="QBF46_000441"
     CDS             complement(46397..46522)
                     /locus_tag="QBF46_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672675.1"
                     /translation="MSFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(46524..47972)
                     /gene="uacT"
                     /locus_tag="QBF46_000442"
     CDS             complement(46524..47972)
                     /gene="uacT"
                     /locus_tag="QBF46_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDQ6672676.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            48408..50327
                     /gene="ygfT"
                     /locus_tag="QBF46_000443"
     CDS             48408..50327
                     /gene="ygfT"
                     /locus_tag="QBF46_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDQ6672677.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGNASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGELGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            50327..50815
                     /locus_tag="QBF46_000444"
                     /pseudo
     CDS             50327..50815
                     /locus_tag="QBF46_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(50851..52218)
                     /gene="ghxQ"
                     /locus_tag="QBF46_000445"
                     /gene_synonym="ygfQ"
     CDS             complement(50851..52218)
                     /gene="ghxQ"
                     /locus_tag="QBF46_000445"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDQ6672678.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(52254..53570)
                     /gene="guaD"
                     /locus_tag="QBF46_000446"
     CDS             complement(52254..53570)
                     /gene="guaD"
                     /locus_tag="QBF46_000446"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDQ6672679.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVNSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(53588..54988)
                     /gene="xanQ"
                     /locus_tag="QBF46_000447"
     CDS             complement(53588..54988)
                     /gene="xanQ"
                     /locus_tag="QBF46_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDQ6672680.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(55153..58023)
                     /locus_tag="QBF46_000448"
     CDS             complement(55153..58023)
                     /locus_tag="QBF46_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDQ6672681.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEISLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(58020..58799)
                     /locus_tag="QBF46_000449"
     CDS             complement(58020..58799)
                     /locus_tag="QBF46_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDQ6672682.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVATTDHDGMRIALDGVASKALRLHDVEKQNLEGNSLEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(58850..60178)
                     /gene="ssnA"
                     /locus_tag="QBF46_000450"
     CDS             complement(58850..60178)
                     /gene="ssnA"
                     /locus_tag="QBF46_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDQ6672683.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(60181..63279)
                     /gene="ygfK"
                     /locus_tag="QBF46_000451"
     CDS             complement(60181..63279)
                     /gene="ygfK"
                     /locus_tag="QBF46_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDQ6672684.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDICGFGY
                     IGLKEESFDHDLKLKQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(63601..64179)
                     /gene="mocA"
                     /locus_tag="QBF46_000452"
     CDS             complement(63601..64179)
                     /gene="mocA"
                     /locus_tag="QBF46_000452"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDQ6672685.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSVRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            64282..65052
                     /gene="yqeC"
                     /locus_tag="QBF46_000453"
     CDS             64282..65052
                     /gene="yqeC"
                     /locus_tag="QBF46_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDQ6672686.1"
                     /translation="MKSIVDSSVLFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFAEITGLTPDATLQLSDLVALVRHPLGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSEFLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            65100..66725
                     /locus_tag="QBF46_000454"
     CDS             65100..66725
                     /locus_tag="QBF46_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDQ6672687.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(66766..67698)
                     /gene="arcC"
                     /locus_tag="QBF46_000455"
     CDS             complement(66766..67698)
                     /gene="arcC"
                     /locus_tag="QBF46_000455"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDQ6672688.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTKQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(67746..69131)
                     /gene="hyuA"
                     /locus_tag="QBF46_000456"
     CDS             complement(67746..69131)
                     /gene="hyuA"
                     /locus_tag="QBF46_000456"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDQ6672689.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(69184..70395)
                     /locus_tag="QBF46_000457"
     CDS             complement(69184..70395)
                     /locus_tag="QBF46_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDQ6672690.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(70453..71649)
                     /gene="dpaL"
                     /locus_tag="QBF46_000458"
     CDS             complement(70453..71649)
                     /gene="dpaL"
                     /locus_tag="QBF46_000458"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDQ6672691.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(71707..72894)
                     /gene="ygeW"
                     /locus_tag="QBF46_000459"
     CDS             complement(71707..72894)
                     /gene="ygeW"
                     /locus_tag="QBF46_000459"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDQ6672692.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYVKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        17664 a        18187 c        19418 g        17906 t
ORIGIN      
        1 attttatttt taccgccgga cggtacccta aaaataaaat aaggataatt tatgcggctt
       61 agtgattatt ttccagaatc atcaatctca gttatacatt cagcaaaaga ttggcaggaa
      121 gctatcgatt tctcgatggt atcattgctg gataaaaact atatcagcga gaattacatt
      181 caggcaatta aagattccac cattaacaat ggcccttatt atattctcgc accaggcgtg
      241 gcaatgcctc atgcgcgacc ggaatgtggg gcgcttaaaa ccgggatgtc tttgatatta
      301 cttgaacaag gtgtttattt tccggggaat gacgaaccaa ttaaattact catcggactc
      361 tctgctgccg atgccgattc gcacattggc gctattcagg cgttaagtga attactgtgc
      421 gaagaagaaa tactcgaaca actcttaaca gcatcatcag aaaaacaatt agcggacatt
      481 atcagccgcg gataattact ctctcctcta caggataata ttatggaaaa caagtctgct
      541 cgtgcaaagg tccaggcttt tgggggcttt ttgactgcaa tggtcatccc caatattggt
      601 gcttttattg cctggggttt tattactgcg ttatttattc ccaccggttg gctgcctaac
      661 gaacatttcg ccaaaattgt cggcccgatg attacctatt tattgcccgt gatgattggt
      721 tctacaggtg gtcatctggt cggcggtaaa cgcggggcgg tcatgggcgg aataggtact
      781 attggtgtga tcgttggcgc agagatcccg atgttccttg gctcaatgat catggggccg
      841 ctcggtgggt tggtcataaa atatgtcgat aaggcactgg aaaaacgcat acctgccggt
      901 tttgagatgg ttatcaataa cttctcatta ggtatcgcgg ggatgctcct ttgtctgctg
      961 ggttttgaag ttatcggccc ggcggtgtta attgccaata ctttcgtcaa agagtgtatt
     1021 gaggcgctgg tacatgcggg ttatctgcca ttgttgtcag tcatcaatga accggcgaaa
     1081 gtgcttttcc tcaataatgc gatcgatcag ggcgtctatt atccgctggg aatgcaacag
     1141 gcttcggtta acggtaaatc catcttcttt atggtggcct ctaacccagg tccgggcctg
     1201 gggctgctgc tggcgtttac cttgtttggt aaagggatga gtaaacgttc tgcgcccggg
     1261 gcgatgatta ttcacttcct cggtgggatc cacgaactgt atttcccgta tgtgctgatg
     1321 aagccgctga ccattattgc catgattgcg ggcggtatgt ctggcacctg gatgtttaac
     1381 ttactggacg gtggtctggt ggctggccca agtccggggt ctatctttgc ttacctggca
     1441 ctgacgccga aaggctcgtt cctggcgaca attgccggtg ttacggtagg taccctggtg
     1501 tcctttgcta ttacttcgct gatactgaag atggaaaaaa cggtggaaac ggagagcgaa
     1561 gatgagtttg ctcagtcagc caatgcggtt aaggcgatga aacaagaggg tgcattctcg
     1621 ttaagcaggg ttaagcgtat cgcctttgtt tgcgatgcgg ggatgggctc cagtgcgatg
     1681 ggcgcgacca ccttccgtaa acgcctggaa aaagcggggc tggcaattga agtaaaacat
     1741 tacgccatag aaaacgtgcc tgcggatgcg gatatcgtcg ttactcatgc cagtctggaa
     1801 gggcgcgtga aacgtgtgac ggataaacca ctgatattga ttaataacta tattggcgat
     1861 ccaaaactcg acactttatt taatcaatta accgccgaac ataaacactg attacagagg
     1921 taaaaatgaa aaccaaagtt gctgctattt atggcaagcg ggatgtccgt ctgcgcgtat
     1981 ttgaactgcc agaaattacc gataatgaat tactggtgag tgtaatttct gacagcgtct
     2041 gtttatcgac ctggaaagcg gcgttactcg gtagtgaaca taaacgcgta cccgacgatt
     2101 tagaaaatca tccggtcatt accgggcatg aatgtgccgg ggttattgtc gaagtgggta
     2161 aaaatctcac tggcaaatat aaaaaaggcc agcgttttgt attgcaaccg gcgatggggt
     2221 taccaagcgg atattcagcg ggctacagct acgaatattt tggcggcaat gccacttata
     2281 tgattattcc cgaaatcgcc attaatttgg gctgcgtatt accgtatcac ggctcttatt
     2341 ttgctgcggc gtcgctggca gagcctatgt gctgcattat tggtgcttat catgccaatt
     2401 atcacaccac gcaatatgtt tatgagcatc gcatgggcgt caaacctggc ggcaatattg
     2461 cactgctggc gtgtgcaggt ccgatgggca ttggcgctat cgattacgcc attaacggcg
     2521 gcatacaacc gtcgcgggtg gtggtggtcg atatcgacga caaacgtctg gcgcaggtac
     2581 agaagctgct gccggtggaa ctggcggcca gtaaaggcat tgagctggtg tatgtgaata
     2641 ccaaagggat gagcgatcct gtccagatgc tgcgggcgct gacaggagat gccgggttcg
     2701 atgacatttt tgtttatgcg gcggtgcctg ctgtcgttga gatggctgat gaattactgg
     2761 cggaagatgg ctgtctgaac ttctttgccg ggccgacgga taaaaacttc aaagtgccgt
     2821 ttaatttcta caacgtccat tacaacagca cgcacgtcgt cggtacatct ggcggttcaa
     2881 cggacgacat gaaagaggcg attgccctta gcgccactgg gaagttacag ccgtcgttta
     2941 tggtgaccca tatcggtggc ctggatgcgg tgccagaaac cgtgctcaat ctgccggata
     3001 tccctggcgg taaaaaactc atttataacg gcgtgaccat gccgctcact gccattgccg
     3061 attttgccga aaaaggcaaa accgatccgc tgtttaaaga gttggcgcgg ctggttgagg
     3121 aaacgcacgg catctggaat gaacaggccg agaaatatct gctggcacaa tttggcgttg
     3181 atatcgggga ggccgcgcaa tgatgtccct ggcgtggcca ttatttcgcg ttacggaaca
     3241 ggcagcgttg gctgcctggc cgcaaaccgg atgtggcgac aaaaataaaa ttgatggcct
     3301 ggcggtcacc gcgatgcgcc aggcattaaa cgacgtcgct tttcgtggac gagtggttat
     3361 cggcgaagga gaaattgacc atgcgccgat gctgtggatt ggcgaagagg tgggcaaagg
     3421 cgatgggcca gaagtcgata tcgcggttga tcccattgaa ggcacgcgga tggtggcgat
     3481 ggggcagagc aatgcgctgg cggtcatggc tttcgcccct cgagatagcc tgttacatgc
     3541 gcccgatatg tatatgaaaa agctggttgt taatcggctg gcggcgggcg cgattgatct
     3601 gtctttgcct ctgacggaca acctgcgcaa cgtggcgaaa gcgttaggca aaccgctgga
     3661 taagctgcgc atggttacgc tggataaacc gcgcctgagt gccgcgattg aggaagcaac
     3721 acagctgggc gtgaaggttt ttgccctgcc ggatggcgat gtcgccgcca gcgtactgac
     3781 ctgctggcag gataatcctt acgatgtgat gtacaccatc ggcggtgcgc cagaaggcgt
     3841 gatttctacc tgtgccgtta aagcattagg cggggatatg caggccgagc tgattgactt
     3901 ttgccaggct aaaggggatt acacggaaaa tcggcagatt gcagagcagg agcgcaagcg
     3961 ttgcaaggca atgggcgttg acgttaaccg tgtgtactcg ctcgatgaac tggtgagggg
     4021 gaacgatatc ctctttagcg ctacgggcgt gacgggcggt gagctggtga acggtatcca
     4081 acagacggcg aatggggtgc ggacgcagac attactgatc ggcggcgcgg accaaacgtg
     4141 taatataata gactccctgc attgatggtg atctatcgga agaccaatgg cgacgctgac
     4201 agaagatgat gtgcttgagc aactggatgc acaggacaat ttattttcat ttatgaaaac
     4261 tgcgcatacc attttgctcc aggggatacg ccagtttctg ccgtcgctgt ttgtcgataa
     4321 cgatgaagag atcgtggaat atgcagtgaa gccgttactc gcccaaagcg gcccgcttga
     4381 cgatattgat gttgcactgc gtttgattta tgcgttgggg aaaatggata aatggttgta
     4441 cgccgatatc acgcattttt cccagttctg gcattacctg aacgaacagg atgaaacgcc
     4501 cggatttgcc gatgacatga cctgggattt tatcagcaac gtcaatagca taacctgcaa
     4561 tgcgacgctc tatgacgcgt tgaaagcgat gaagttcgcc gatttcgccg tctggtcaga
     4621 ggcgcgtttt agcggaatgg tcaaaacggc gctgacgctg gcagtaacga ccaccttaaa
     4681 ggaattaacg ccgtgaaaat tgaattaacg gtgaatgggc tgaagataca ggcacagtac
     4741 cagaacgaag agattgaaaa tgtacataaa ccgttgctgc acatgttggc ggccttacag
     4801 acggtaaatc cgcagcggcg tacggtggtt tttctttgcg ctccgccggg gacgggtaaa
     4861 tcaaccctaa ccaccttctg ggaatatctc gcgcaacaag acccggaact gcccgctatt
     4921 cagacgctcc cgatggatgg ttttcaccat tacaatagct ggctggatgc gcatcaattg
     4981 cgccccttca aaggcgcacc agagacattt gacgttgcga aactgacgga aaatctgcgc
     5041 caggtcgtgg aaggggattg tacgtggccg cagtacgatc gacaaaagca tgatcctgtt
     5101 gaagatgcgt tgcacgttac cgcaccactc gtcatcgtcg aaggaaactg gttgttactg
     5161 gatgatgaaa agtggctgga gttggcatct ttctgcgatt tttctatttt tattcatgca
     5221 cctgctcaaa ttctgcggga gcgcttgatt agtcgtaaaa ttgcgggagg attaacgcgg
     5281 caagtagccg aagcatttta tgcccgcacc gatggcccca atgtcgagcg tgtgctgatg
     5341 aatagccgtc aggccaatct gatagtagag atgactgaag aaggccggta tcacttcaca
     5401 agctgacaat ttattccagt tattgtaggg ttattattac gccagcctgg aataaagctt
     5461 cccacaaaag gggataaaag tgtgatgtga gtcagataaa tgtcttcttc ggctggacaa
     5521 acattccttt tattccacgt ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt
     5581 tcgacaatta accaatcagt cgcagtttgc gacaggtaag gtttccccgg acgatttgct
     5641 ggattactct gtctggcaaa tttgctcagg gaaaaccttg caggagatct atgaccgtac
     5701 gcgtagcgat aaatggcttc ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat
     5761 ccggacgccg ggcggaaatt accgtggtgg caatcaacga actggcggat gctgcgggca
     5821 tggcgcattt gttgaaatat gacaccagcc atggccgttt tgcatgggaa gtacgacagg
     5881 aacgcgatca actttttgtt ggtgatgacg ccatccgcgt attgcatgaa cgttcactgc
     5941 aatcgctccc ctggcgtgaa cttggcgttg atgtagtcct cgactgcacc ggcgtatatg
     6001 gctcccgcga gcatggcgaa gcgcatattg ccgccggggc caaaaaagtg ctcttttcac
     6061 atcctggcag taacgatctc gacgcgaccg ttgtttacgg cgtcaatcag gatcaacttc
     6121 gtgcggaaca ccgcatcgtt tctaacgctt cctgtaccac gaattgcata attcccgtca
     6181 tcaaattgtt agatgatgcg tacggtattg agtccggcac tgtgaccaca attcactccg
     6241 ccatgcacga tcaacaggtt attgatgcat accatcctga cctgcgtcgc acccgggcag
     6301 ccagccagtc gatcattccg gtcgatacta aactggccgc cggtatcaca cgattttttc
     6361 cgcaatttaa cgatcgcttt gaagcgattg cggtacgtgt gccaaccata aatgtgacgg
     6421 caatcgattt aagcgtgacg gtgaaaaaac ctgtaaaagc caatgaagtc aacctgttgc
     6481 tgcaaaaagc agcacaaggt gcatttcatg gtatagttga ctatacggaa ttgccgttgg
     6541 tctctgtaga ttttaaccac gatccgcaca gtgccattgt cgatggcacc caaacccggg
     6601 tcagtggcgc acacctgatc aaaacgttgg tctggtgcga taacgaatgg ggctttgcta
     6661 accgaatgct cgacacgacg ttagctatgg ctactgttgc tttcaggtaa gacgcaagca
     6721 gcgtctgcaa aacttttaga atcaacgaga ggattcacca tgtctgtaat taagatgacc
     6781 gatctggatc ttgctgggaa acgtgtattt atccgtgcgg atctgaacgt accagtaaaa
     6841 gacgggaaag taaccagcga cgcgcgtatc cgtgcttctc tgccgaccat cgaactggcc
     6901 ctgaaacaag gcgcaaaagt gatggtaact tcccacctgg gtcgtcctac cgaaggcgag
     6961 tacaacgaag aattctctct gctgccggtt gttaactacc tgaaagacaa actgtctaac
     7021 ccggttcgtc tggttaaaga ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt
     7081 gttctggaaa acgttcgctt caacaaaggc gagaagaaag acgacgaaac cctgtccaaa
     7141 aaatacgctg cactgtgtga cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg
     7201 caggcttcta ctcacggtat cggtaaattc gctgatgttg cgtgcgcagg cccgctgctg
     7261 gcagctgaac tggacgcgct gggtaaagca ctgaaagaac ctgctcgccc gatggtggct
     7321 atcgttggtg gttctaaagt atctaccaaa ctgaccgttc tggactccct gtctaaaatc
     7381 gctgaccagc tgattgttgg tggtggtatc gctaacacct ttatcgcggc acaaggccac
     7441 gatgtgggta aatccctgta cgaagctgac ctggttgacg aagcaaaacg tctgctgacc
     7501 acctgcaaca tcccggttcc gtctgatgtt cgcgtagcaa ctgagttctc tgaaactgca
     7561 ccggctaccc tgaaatctgt taacgatgtg aaagctgacg agcagatcct ggatatcggt
     7621 gatgcttccg ctcaggaact agctgaaatc ctgaagaatg cgaaaaccat tctgtggaac
     7681 ggtccggttg gcgtgttcga attcccgaac ttccgcaaag gtactgaaat cgtggctaac
     7741 gctatcgcag acagcgaagc gttctccatc gctggcggcg gcgacactct ggcagcaatc
     7801 gacctgttcg gcattgctga caaaatctcc tacatctcca ctggcggcgg cgcattcctc
     7861 gaattcgtgg aaggtaaagt actgcctgca gtagcgatgc tcgaagagcg cgctaagaag
     7921 taaaaaatca cagggcaggg aaacctgccc ttgtttcagc gcgcttttag agcacgcatt
     7981 ttttcatact ctaaataatt cgagttgcag gaaggcgaca agagagtgaa tccccaggag
     8041 cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta
     8101 tgacgagtat aaggcccgac gatacaggac aagagacatg tctaagattt ttgatttcgt
     8161 aaaacctggc gtaatcactg gtgatgacgt acagaaagtt ttccaggtag caaaagaaaa
     8221 caacttcgca ctgccagcag taaactgcgt cggtactgac tccatcaacg ccgtactgga
     8281 aaccgctgct aaagttaaag cgccggttat cgttcagttc tccaacggtg gtgcttcctt
     8341 tatcgctggt aaaggcgtga aatctgacgt tccgcagggt gctgctatcc tgggcgcgat
     8401 ctctggtgcg catcacgttc accagatggc tgaacattat ggtgttccgg ttatcctgca
     8461 cactgaccac tgcgcgaaga aactgctgcc gtggatcgac ggtctgttgg acgcgggtga
     8521 aaaacacttc gcagctaccg gtaagccgct gttctcttct cacatgatcg acctgtctga
     8581 agaatctctg caagagaaca tcgaaatctg ctctaaatac ctggagcgca tgtccaaaat
     8641 cggcatgact ctggaaatcg aactgggttg caccggtggt gaagaagacg gcgtggacaa
     8701 cagccacatg gacgcttctg cactgtacac ccagccggaa gacgttgatt acgcatacac
     8761 cgaactgagc aaaatcagcc cgcgtttcac catcgcagcg tccttcggta acgtacacgg
     8821 tgtttacaag ccgggtaacg tggttctgac tccgaccatc ctgcgtgatt ctcaggaata
     8881 tgtttctaag aaacacaacc tgccgcacaa cagcctgaac ttcgtattcc acggtggttc
     8941 cggttctact gctcaggaaa tcaaagactc cgtaagctac ggcgtagtga aaatgaacat
     9001 cgataccgat acccaatggg caacctggga aggcgttctg aactactaca aagcgaacga
     9061 agcttatctg cagggtcagc tgggtaaccc gaaaggcgaa gatcagccga acaagaaata
     9121 ctacgatccg cgcgtatggc tgcgtgccgg tcagacttcg atgatcgctc gtctggaaaa
     9181 agcattccag gaactgaacg cgatcgacgt tctgtaagtc attcctttct gcttatctca
     9241 aggcccgctc tgcgggtctt tttttcgcca aaagcaaaat tgcctgatgc gctatgctta
     9301 tcaggcctac acgtatcctg caatatattg aatttgcagg tttttgtagg ccggataagg
     9361 cgttcacgcc gcatccggca tgacaaagcg cacattgata tctgtctgag ccccgctctg
     9421 cggggatttt tttgccaaaa gcaaggtgat tcagatgaga aatctgtgat ctatttggca
     9481 aaattatgct ttattgttta cccttgtcag actgcccgtc ataaggcggc ggagtgtatt
     9541 tctccatttt gagtcagttg aaaaggaata ttgaatggaa gatttgaatg ttgtcgatag
     9601 cataaacggc gcgggaagct ggctggtagc taaccaggcg ctgctgctaa gttatgcagt
     9661 aaacatcgtg gcggcactcg cgatcatcat cgttggtttg attatcgcgc ggatgatttc
     9721 caacgcggtg aatcgcctga tgatctcccg taaaatcgat gccactgttg ctgattttct
     9781 ttctgcatta gtccgttacg gtattatcgc ctttacgcta atcgcagccc tgggacgcgt
     9841 gggtgtacaa acagcgtcag tcattgctgt actcggtgcc gcaggcttag ctgttggtct
     9901 ggctttgcag gggtcacttt ctaacctggc cgctggcgtg ttacttgtca tgttccgccc
     9961 gttccgtgcc ggagaatatg ttgacctcgg cggcgtagcc ggtactgtgc tgagtgtgca
    10021 gattttctcc accaccatgc gtactgcaga cggtaaaatt atcgttattc cgaacggtaa
    10081 aattattgcc ggaaatatta ttaacttctc ccgcgagcca gttcgccgta acgaatttat
    10141 tattggcgtg gcgtatgatt ccgatatcga tcaggttaag cagatcctga ccaatattat
    10201 ccagtctgaa gatcgcattt tgaaagatcg cgaaatgact gtgcgcctga acgaacttgg
    10261 tgcatcgtcg attaatttcg tggtccgcgt ctggagcaac agcggcgatc tgcaaaacgt
    10321 gtactgggat gtgctggagc gtattaaacg tgaatttgat gccgccggta tcagcttccc
    10381 gtacccgcaa atggatgtga actttaagcg ggtgaaagaa gacaaagctg cgtaatcaac
    10441 gctgaggcca gataatactc tctggcctct ctcttattag ttattctgat tgccaattaa
    10501 tattatcaat ttccgctaat aacaatcccg cgatatagtc tctgcatcag atacttaatt
    10561 cggaatatcc aacgtgtttt cttattactt tcaaggtctt gctcttgggg cagctatgat
    10621 tctgccgctc ggcccacaaa atgcttttgt gatgaatcag ggcattcgtc gtcagtacca
    10681 cattattatt gccttacttt gcgctatcag cgatttggtc ctgatttgcg cggggatttt
    10741 tggtggtagc gcgttattga tgcagtcgcc gtggttgctg gcgctggtca cctggggcgg
    10801 cgtagtcttc ttgctgtggt atggttttgg cgcttttaaa acagcgatga gtagtaatat
    10861 tgagttagct agtgccgaag tcctgaagca aggcagatgg aaaattatcg ccaccatgtt
    10921 ggcagtgacc tggctgaatc cgcatgttta cctggatact tttgttgtac tgggcagcct
    10981 tggcgggcag cttgacgtcg aaccaaaacg ttggtttgcg ctcgggacaa ttagcgcttc
    11041 tttcctgtgg ttctttggtc tggctattct cgcagcctgg ctggcaccgc gcctgcgcac
    11101 ggcaaaatca cagcgcatta tcaatctggt agtgggatgt gtcatgtggt ttatagcctt
    11161 gcagctggcg agagacggta ttgctcatgc acaagccttg ttcagttagg cgctatctga
    11221 tggaaaaata aaacagaggc gctaagcttg cctccagagg tcctgaattt tgggcaagtc
    11281 aattaacacg gagagactaa cgtgaagttc aaagttatcg ccctggcggc attaatgggt
    11341 attagcggga tggcagcgca ggctaacgaa ttgccggatg gaccgcatat tgtcacctcc
    11401 ggtacggcaa gtgtggatgc ggtgccagac attgccactc ttgcgattga agttaacgtg
    11461 gccgcgaagg atgccgctac agccaagaaa caggcagatg agcgcgtcgc acaatacatt
    11521 tccttccttg aactcaatca gatcgcgaaa aaagatatca gctcagcgaa tttacgcacc
    11581 cagccagatt atgattatca ggatggtaaa agtatcctga aaggctaccg cgctgtgaga
    11641 acggtggaag tcacgctccg tcagttagac aaactgaatt ccttgctgga tggcgcgctg
    11701 aaggcgggtc ttaacgaaat tcgttcagtg tcgctgggcg tggcgcagcc ggatgcctat
    11761 aaagacaaag cgcgtaaggc agcgattgat aacgcgattc atcaggcgca ggaactggcg
    11821 aacggctttc atcgtaaact ggggccggta tatagcgtgc gctaccatgt ttccaactat
    11881 cagcccagcc caatggtgcg gatgatgaaa gccgatgccg cgccggtgtc tgcccaggaa
    11941 acttacgagc aggccgctat tcagtttgat gatcaggtcg atgtggtctt ccagttagaa
    12001 cctgtggatc aacaacccgc taaaacacct gcagcacaat aatacttgta gcccgacgta
    12061 ttcgtcgggc taatgccttt acccgatatt gcctcctttt tctgtaattt tctgtaaaac
    12121 catgacgtaa tcacctatct ttgctcgcgg tttcatcaag gataggctga ctgaaacggt
    12181 taagcggaca aggatgcggg gaggggggat ggacattttt atttcgaaaa agatgcgcaa
    12241 tttcatttta ttagcgcaaa ccaataatat tgcccgggcg gcagaaaaaa tccatatgac
    12301 ggcttcgcca tttggtaaaa gcattgccgc gctggaagaa caaattggct atacgctatt
    12361 tacccgcaaa gataataata tcagcctcaa taaagccggt caggaactgt atcaaaaact
    12421 gttcccggtt tatcaacgac tttctgcgat tgataatgaa atccataact cagggcgtcg
    12481 ttcacgagaa attgtgattg gcatagataa tacttatccg acaattattt tcgatcagtt
    12541 gattagtctc ggagataagt atgaaggcgt gaccactcag ccagttgagt tcagcgaaaa
    12601 cggcgttatc gataatcttt ttgaccgtca gttagatttt attatctccc cacagcatgt
    12661 atctgcacga gttcaggaac tggaaaacct gactatcagt gaattaccac cgctgcgatt
    12721 gggctttctc gtttctcgcc gttatgaaga gcgtcaagag caagagctgc ttcaggagct
    12781 gccctggcta caaatgcgct ttcaaaaccg ggctaacttc gaagcaatga ttgatgcgaa
    12841 tatgcgacct tgtgggatca atccaaccat tatttatcgt ccctacagtt ttatggccaa
    12901 aatcagcgcc gtggagcgcg gacattttct gacggtgatc ccgcattttg cctggcgact
    12961 ggtgaatccg gcaacgctga aatattttga tgcaccgcac aggccgatgt atatgcagga
    13021 atatctttat tcaatcagaa atcatcgtta taccgccacg atgcttcagc atattgctga
    13081 agatcgtgac gggacgagtc attaacccag catcgagccg gttgcaatta aattacggtg
    13141 taagtcgaag acgtggctaa gatcgtggtg aatatgtccg ccaggcgcat tttccagata
    13201 gcgatgcaga taatcccgat acataggatg tgcacaatta tcaatgatag tgcgggcgcg
    13261 ttgaagcggg gaaagaccgc gcagatcggc gatcccttgt tcggtgatga tcactttgac
    13321 gctgtgttcg ctgtgatcaa catggctgca cattggcacg acggttgaga tcttgccttc
    13381 tttagcaatc gacggggcca taaagatcga cagatacgcg ttgcgttcaa aatcaccgct
    13441 gccgccgatg ccgttcatca gatcgacccc ggctacgtgt gttgagttgg catgcccgta
    13501 aatatcaaac tccaggccga cgttcagagc gatgacgccc agacgacgga tgatttccgg
    13561 gttattggaa atctcctgcg gacgcaacac aatgcggctg gcaaagtaat ccatattgtc
    13621 gtaaatcttg cgcagggaat cggccgagat tgtcaggctg gaggcgctgg ccccgctgat
    13681 tttgccggtt tccagtaaat gcaccaccga ttcctgtagc acttccgaat acatcataaa
    13741 cggaggaatt tccgggtttt cccccagacg cgccattacc gcattattga tattgcccac
    13801 gccactttgc agcggcagaa attccggcgg aatacgccca tgcgccattt cctgcaataa
    13861 gaacgtgacc acgttatcgg caatctgctg gcacatggga ttttgcttat ccagcatatt
    13921 accggcgtca ggcaagttgg tttccacgac ggcgacaatc tttttcggat cgatttgcac
    13981 atagcgggta ccgacgcgat ccattgcatg gaagatcgac acgctattgc gccgcggtgg
    14041 cgcgccagga atcacaatat ccgccagttc tgcaacgcgc ggatcgtgat agtgattgag
    14101 ttcaatgatc actttcttcg cccgcagcag ccatgtcggc gcattaccga tcccgctggt
    14161 taaccagact cgaccatctg gtgccagtgc cgatgcttca atgacggcaa catcaatgtc
    14221 gccgaagaaa ccgtaattga ccatttgcgc cacttcgctc aaatgaaggt caacgaaact
    14281 caccgcgccc tgattgatct ttttacgtaa accggacgat gtttgatatg gcgcacgcca
    14341 ggaaacagca tcggcgtcag aaagtacatc gtcagcggcg gcgctgattg acgcccccgt
    14401 cagcaggcga atttgatacg gctttttggc ctcatgctgt tcgttagctc tgtgggcaat
    14461 cgcggtgggt agggctttcg gcgaacccgc tggggtaaag ccgctaaatg ccaccatgtc
    14521 gttatgctgg ataatttctg ccgcttcatc ggcggtcatc cttgtccact gagtttccat
    14581 ttttactcct tcgttcgcag ggattaatga ccaacgaaat taggtttacg tttttcgagg
    14641 aaagcgttca ttccttcctg gtaatcttcg ctgtcataaa ccgcgcgtcg catcccctga
    14701 atacgttcaa attcatcgga gttcatggtg tgcgcttcgc ccagtacacg cagctcttct
    14761 ttgataacag caatggctaa cggcgctttc tcagagatgt ggtgcgccat ttgtaaggtg
    14821 aaatcttcca gttcttccac ttccacaaca tggttgagga tgccgacagc caacgcgcgc
    14881 tgggcggtga ttggcgaagc ggtaaaaatc agttctttga caatgtggaa gcccgcgtcg
    14941 cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc cgaggtttac aggcgtcatt
    15001 gagaaggttg aggtactggc ggcgataatc agatcggaac tcatgatcat ttcaaatgcg
    15061 ccaccccaaa cactaccttc caccatcgaa atgatcggtt tcgggaattt ttggatcatg
    15121 cgggtgattt gacgcaatgg atcatcatag gagagcggat cgcgaccgcc agacggcagt
    15181 tcgtgaatat cgtggcctgc ggagaagact ttggatccac tcggcgcgcg caaaatgata
    15241 cagcgaattt ccggccggtt gagatcgctt aatgcctgca taagatcatc aataaagact
    15301 ttacttaagg cattaagttt gcggccatag ttaaactcaa tgaccgccac tttgttgata
    15361 gtgacaacgt taacatactg ataagacata aaaattcctt taatcaaaat attgcgtctg
    15421 gataaattca ctgagctgcc gcaggccggt gcgcggtgag agcgtattgt ttttgaccgc
    15481 taaaagcgtc tggcggtaat agcgatcgaa atcttcattc gcgaacaggt gattcagtac
    15541 ttcttcttcg gtctgcttac gcagccattc caccgattgt tgttgccgca cttgttgtaa
    15601 acgaccactg gcagttagcg cggttttgaa gtcgatgatg gcgtgccaga tctcatcgat
    15661 tccacgtttt tccagtgcgc tacaagtcag aacccgtggc tgccattcgt cgtatttacg
    15721 tcgcagaata tgcagggcac tctcgtacat atgccgggca atggcgacat tggtatggtt
    15781 atcgccatcg tctttgttga taacgatcag atcagccact tccatcagcc cttttttaat
    15841 gccctacaga tcatcgccgc caccggcaat ttgcaacgag ataaaacagt ccaccatgcg
    15901 ggcgacttct gtttccgact gcccgacgcc aaccgtttcg acaatcacta cgtcataacc
    15961 cgctgcttcg cataacagca ttaattcccg cgctcgctga ctggcaccgc ccagatgacc
    16021 ggaggatggt accgggcgaa taaacgccgc ttcggcacgc gccaggtcat tcatgcgggt
    16081 tttatccccg agaatgctac cgccagtgac cgggctgctg ggatcgaccg caataaccgc
    16141 gacctttaat ccctctcgaa tcaacaacat gccaaaggcc tcaagaaagg tacttttccc
    16201 cgcgccgggg gtgccggtaa cgcccagtcg cagggtgtta ccgcagtacg gcataatggc
    16261 atcaagcagc tgcgtactta gtgcctgatg acgcgggtga cggctttcca ccagcgtcat
    16321 ggcctgggcg agtgtggcac gctcaccctg acgtaagcgg cgaatacttt ctgccagcgt
    16381 ggcttcatta atcatgatgc tggcttatca gattcagtac gtcgcgcaca ctgtcgagca
    16441 taggtgtacc tggaccataa atcgccgcac gccgcgctct tgcaggaagg cgtaatcctg
    16501 cggcggaatg acgccacccg cgaccacgca gatatcttcg cgtccccatt ttttcagcgc
    16561 ttcgaccagt tccgggatca gcgttttatg accggcagcc agtgaggatg cgcccactac
    16621 gtgaacgtcg ttttctacgg ccaggcgggc gatctcttca ggtgtagaga acatcgggct
    16681 taaatctacg tcgaaaccga gatcggaata ggcgctggcg atcactttcg cgccgcgatc
    16741 gtgtccatcc tggcccatct tagcgatcag aatgcgcggg cgacgaccat tgtcggcaag
    16801 gaactgctcc gtttgcgcaa caatggcatc gaactcggag gccgatttct cagactgatg
    16861 atagctttgc gcaatcacgc cggtaacaca ctggcttggc accagataac ggtcgaaagc
    16921 gacttccagc gcatcggaaa tttcacccag ggtggcgcga acgcgagcgg cattaacagc
    16981 ggcagccagc aggttttcgt tatgctgtgc ggcgtgagtc agggcgttca acgcggcggt
    17041 tacggcggca tcatcacggg tggcgcgaat gcgttccagc gaagcaattt gctcgttacg
    17101 caccatcacg ttgtcgatct caagtacatc ggtttcgtct tcgtgatcca gtttgtactt
    17161 gttgacacca acgatgacac gcttgccctg gtcgatcagc gactgttcgc gcgctgaggc
    17221 ctcttcgatc attcgttttg gcagacctgc ttcgatcgct ttcgccatgc caccggcttc
    17281 gtcgatctgt tggataatag ctctggcttg tttgacgatt tgatcggtca gcgactcaat
    17341 gtaataggat ccggccagtg gatcgacggt gcggcagagt tctgattctt cctggatgat
    17401 gatctgggtg ttgcgggcaa tgcgtgctga gaaatcggta ggcaaaccaa gcgcttcgtc
    17461 aaaggcgttg gtatgcagtg actgagtacc gcccagcgtc gcagccagcg cttcaatggt
    17521 ggtgcggata acgttgttat acggatcctg ttcagtcagg ctctaagcct gaggtctggc
    17581 agtgggtacg cagcgccagt gatttcgggt cctgtgcgcc aaatccactg accgcttcgc
    17641 tccataaata acgtgccgca cgcaacatgg cgacgttcat aaacagatcc atgccgatgc
    17701 cgaagaagaa cgacaggcga ggagcgaagt catcaatttt cagtccggca gagattgctg
    17761 ctttgatgta ctcaatccca tcagcgagcg taaatgctac ctgctgcacg cagttggcac
    17821 ccgcttcacc catgtggtaa ccgctgatac tgatggtatt aaatcgcggc atgttgccgg
    17881 aacaccaggc gatgatgtcg gcgataatgc gcattgacgg ttttggcggg taaatatagg
    17941 tgttgcggca gaggtactct ttgaggatat cgttctgaat ggtgccggtc agtttatcag
    18001 gtgtaacacc ttgctcttct gcggcgacga tataaaacgc cagtactggt agcactgcgc
    18061 cattcatggt catcgaaacc gacattttat ccagcgggat ctggtcgaac aggactttca
    18121 tatcttccac ggtgtcgata gcgacgcccg ctttgccgac gtcgcccgcc acgcgcgggt
    18181 tatcggagtc gtagcctcgg tgggtggcaa ggtcaaacgc aacggaaaga cctttttgcc
    18241 cggcggccag gttacggcga taaaaagcgt tggactcttt tgctgttgaa aaaccagcat
    18301 actgacggat ggtccacggt tgggcggtat acatagtggc acgcgggcca cgaacgtagg
    18361 gcggcaaacc aggaagggta cctgtcacct ccagattatc gagatcggct tcggtataca
    18421 gcggcttgat ggtgatccct tccgcggttt gctgaaccag cgagtcgaca gttttctccc
    18481 gacggctcaa ttccttgttg gcaagctgtt gccactcctg cacgttagac attatttgct
    18541 ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg tgagcttttt acgtgggttt
    18601 gtcgccagca aaaaaaatgc attcagacgt cgttttttgc cgcctgagat ttgtctattt
    18661 tgtgatgtga ttcaaggaat acaaaaagaa gtgaggataa cgcctgatat gtgctatcag
    18721 gcattatttg atggattaat cctgacgaag gactttgtga ccataatcga gcaacgcatc
    18781 agtgacttta cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga gcatccggcg
    18841 ttgaaatagc cctggcgtta agtcaatcag ttcaccgctg gctagttctt tctcgatttg
    18901 caggtgcggg atcatacagc aggtggtgcc ctggcgagca agttgcacga acgcttctga
    18961 agaattaacg atatggcagg gcacgctgcc tggaggcaga tcgaagtttt gctgcaaaaa
    19021 ggcctggtgc atatcgtcaa gatggtcaaa ggcgaccaca ggtgctttca ataatgccga
    19081 acgcgttacg ccgttaggga aatatttttc ggcaaacggt tttgagctga cgaacagata
    19141 gtcaagcgca ccgagcttat ctacaaggca actcggcagc gcctgatgtt gaatactcac
    19201 cgcgccgacc acttcgccgc ggcgcagacg ttcctgagta cgggtttcat cttctacctg
    19261 caagttgagg cggataggcg aatcagccaa cacaggagcc agtgccggaa gcaaccacgt
    19321 cgccagactg tcggcgttga ccgccagtga aagcagcagc ggagtcgaac cggtttgttc
    19381 atcgcccagc cactcttctt ccagcaactc cacctggcgc agcagtgcca gcagtttttg
    19441 cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac agcggctgcc cgaacatatt
    19501 ttccagttgc ttaatgcgct gtgagacggc tgattgtgta atgcacagct tttgtgccgc
    19561 gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc tgtagtccgg
    19621 gcgtttcatt gctgttgtct gctcctggta aatgcttttt cctgcactat gacacaattt
    19681 tgtgtcaggt tgcatatacc gcagccgtcg gcgggtaaca tttgtatcaa tggttaaacg
    19741 cgcagcttaa aagatcaggg atttgcgcta ttgcatttcg cttagcgact tcgcgccctg
    19801 gttatgttga atggcgtggc gttattgcct ctgttcgcac cgtggagcgg catacccaga
    19861 ccgttgtatg ctttatttgc ggggcgaaag gggatgcccg ccattgcgcg atattttatg
    19921 gatgagttaa ccacgcggct tgccaacggg gtctgaatcg ctttttttgt atataatgcg
    19981 tgtgaaattt cataccacag gcgaaacgat catgacgcag gatgaattga aaaaagcagt
    20041 aggatgggcg gcacttcagt atgttcagcc cggcaccatt gttggtgtag gtacaggttc
    20101 caccgccgca cactttattg acgcgctcgg tacaatgaaa ggccagattg aaggggccgt
    20161 ttccagttca gatgcttcca ctgaaaaact gaaaagcctc ggcattcacg tttttgatct
    20221 caacgaagtc gacagccttg gcatctacgt tgatggcgca gatgaaatca acggccacat
    20281 gcaaatgatc aaaggcggcg gcgcggcgct gacccgtgaa aaaatcattg cttcggttgc
    20341 agaaaaattt atctgtattg cagacgcttc caagcaggtt gatattctgg gtaaattccc
    20401 gctgccagta gaagttatcc cgatggcacg tagtgcagtg gcgcgccagc tggtgaaact
    20461 gggcggtcgt ccggaatacc gtcagggcgt ggtgaccgat aatggcaacg tgatcctcga
    20521 cgtccacggc atggaaatcc ttgacccgat agcgatggaa aacgccataa atgcgattcc
    20581 tggcgtggtg actgttggct tgtttgctaa ccgtggcgcg gacgttgcgc tgattggcac
    20641 acctgacggt gtcaaaacca ttgtgaaatg atctgacggg ggaacctccc ccgttaaaaa
    20701 aattctcttc attaaatttg gtgacatgtg tcacgctttt accaggcaat tgtcgattgc
    20761 tctaaataaa tcctctaaac cagcatattc atccaagaat tacctttgcg tgatatttcc
    20821 tcaacatcgc gacgcaaacg ttcatattgc cgcaatatta ttttttgata tgttgaaagg
    20881 cggatgcaaa tccgcacaca acatttcaaa agacaggatt gggtaaatgg caaaggtatc
    20941 gctggagaaa gacaagatta agtttctgct ggtagaaggc gtgcaccaaa aggcgctgga
    21001 aagccttcgt gcagctggtt acaccaacat cgaatttcac aaaggcgcgc tggatgatga
    21061 acaattaaaa gaatccatcc gcgatgccca cttcatcggc ctgcgatccc gtacccatct
    21121 gactgaagac gtgatcaacg ccgcagaaaa actggtcgct attggctgtt tctgtatcgg
    21181 aacaaaccag gttgatctgg atgcggcggc aaagcgcggg atcccggtat ttaacgcacc
    21241 gttctcaaat acgcgctctg ttgcggagct ggtgattggc gaactgctgc tgctattgcg
    21301 cggcgtgccg gaagccaatg ctaaagcgca ccgtggcgtg tggaacaaac tggcggcggg
    21361 ttcttttgaa gcgcgcggca aaaagctggg tatcatcggc tacggtcata ttggtactca
    21421 attgggcatt ctggctgaat cgctgggaat gtatgtttac ttttatgata ttgaaaacaa
    21481 actgccgctg ggcaacgcca ctcaggtaca gcatctttct gacctgctga atatgagcga
    21541 tgtggtgagt ctgcatgtac cagagaatcc gtccaccaaa aatatgatgg gcgcgaaaga
    21601 gatttcgcta atgaagcccg gctcgctgct gattaatgcc tcgcgcggta ctgtggtgga
    21661 tattccggcg ctgtgtgatg cgctggcgag caaacatctg gcgggggcgg caatcgacgt
    21721 attcccgacg gaaccggcga ccaatagcga tccatttacc tctccactgt gtgaattcga
    21781 caacgtcctt ctgacgccac acattggcgg ttcgactcag gaagcgcagg agaatatcgg
    21841 cctggaagtt gcgggtaaat tgatcaagta ttctgacaat ggctcaacgc tctctgcggt
    21901 gaacttcccg gaagtctcgc tgccactgca tggtggtcgt cgtctgatgc acatccacga
    21961 aaaccgtccg ggcgtgctaa ctgcgctgaa caaaatcttc gccgagcagg gcgtcaacat
    22021 cgccgcgcaa tatctacaaa cttccgccca gatgggttat gtagttattg atattgaagc
    22081 tgacgaagac gttgccgaaa aagcgctgca ggcaatgaaa gctattccgg gtaccattcg
    22141 cgcccgtctg ctgtactaat tccccttctc tgaaaattaa cgggcaggtc actgacttgc
    22201 ctgttttttt atcccttctc cacaccgttg ccgcgttgcg cattctactt tgcgagtctg
    22261 ctccagaaaa taaatcaaac tcagatcaat gcgttgtgtt tatttaatgg ggatttacga
    22321 tggcgggaca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttaaaggag
    22381 aagggttatg atgcgacttg tcatcatact gattgtactg ttactgataa gtttcggagc
    22441 ttattaacag tcagtctcag gggaggagtc atcctccctt acccttactc actaaattag
    22501 gtcaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    22561 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    22621 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    22681 tgtaaggtcc gatcataaaa accaccgccc attcccaggc gctgaccgta ctcatcaaag
    22741 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    22801 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    22861 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    22921 gcgcgccaga gttgttctat gagtggctgg gtgtcgagtt cgccatcaaa agagaggaat
    22981 acagcgaccg tatgtgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    23041 tgacccattt cctgctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    23101 cggatttctt gtcgggataa tgtcagtggg agttctggta gttgcgtcat ggctggtgcc
    23161 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    23221 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacgg accgagcatg ctcaccaacc
    23281 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    23341 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    23401 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    23461 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtcttcgctt
    23521 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    23581 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgcagcct
    23641 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    23701 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    23761 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    23821 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    23881 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    23941 ctgaaccaac aagggacggg tctgacccct gctgagatgc atggtttaat cagtgggatg
    24001 atatgtggcg gtaacgatga cagctcatgg ctaccgctac ttcacgacct gacgaacgaa
    24061 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    24121 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    24181 agcgttttcg atcgggctga tgcgctggca ggttgggtca atcacttcct gcttggtctt
    24241 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    24301 ctgcgtaaca tcgcgcagtt gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    24361 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    24421 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    24481 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    24541 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    24601 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    24661 ctttaatgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    24721 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    24781 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    24841 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    24901 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    24961 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    25021 gttcaaatca ccagaagaga ttgccgtact tcgccgcgcg ggggaaatca ccgccatggc
    25081 acatacccgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    25141 aattcaccac gaatttaacc gccacggtgc gcgctatccg tcctataaca ccattgtcgg
    25201 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagtgtgaaa tgcgcgacgg
    25261 cgacctcgtg ttgattgacg cgggttgcga atacaaaggt tacgctggcg atattacccg
    25321 caccttcccg gtcaacggca aattcactca ggcccagcgt gaaatctacg acattgtgct
    25381 ggagtctctc gaaaccagct tgcgcctgta tcgtccggga acttccattc tggaagtcac
    25441 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcattc tgaaaggtga
    25501 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    25561 ctggttaggg ctagatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    25621 ggaaccgggc atggtactga ccgtagagcc agggctgtat attgcgccgg atgcagaagt
    25681 gccagaacaa tattgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    25741 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    25801 ggcagctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    25861 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    25921 accgcgccag agtcacatgc tcatccgggc tttgatggac gagcgattgc gctggcggcg
    25981 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggcgga ttgcgcaacc
    26041 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgccg gattcgtcac cctcgccgca
    26101 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcacaatgt cgggcaacgg
    26161 ctgtttgcat tgctgcgcaa agtacctggc gtaacgctgc attgccctga tcgcgtggct
    26221 aacgttgccc gtactcagag tcaagttgaa gtgacgctgg agagtggcga gacgctgacg
    26281 ggccgcgtgc tggtagccgc tgatggcacc cattcagcgt tagccactgt gtgcggcgtt
    26341 gactggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc gacttctgtt
    26401 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    26461 atgtctgatg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    26521 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt cggcctttgg ctggcgactt
    26581 ggaaaaatta cccacgccgg taaacgcagt gcttatccgc tggcgttaac ccgcgccgcc
    26641 agagctatta cccatcgtac cgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    26701 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    26761 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    26821 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcatgg acagcctggt acatcttttt
    26881 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    26941 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    27001 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggtg gcggcatggt
    27061 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    27121 gcgcgtaccg gaacctctgg cggcggatgc accaccacaa ctgcgcgttt cggctatcaa
    27181 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tctctcgtag
    27241 ggccagttgt tatcacggta tggaagtgtg ggacaaagac agttttggtc acatttcgtt
    27301 tgacgatcaa agcatgggct atagccatct tgggcatatc gttgaaaact cagtgattca
    27361 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatc actctgttgg cccccgcaga
    27421 attacagcag gtcgcctggg gagaaaatga aactttcctg acgctgaaag atggcagcat
    27481 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    27541 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    27601 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    27661 tttaccgctt agcgatccgc atctttgctc aattgtctgg tcactgtcgc cagaggaagc
    27721 gcagcggatg cagcaggcaa gtgaagatga atttaatcgc gcgttaaata tcgcttttga
    27781 taatcgcctg ggcttatgca aggttgagag cgcgcgtcag gtgttcccac tgacggggcg
    27841 ttatgcgcgc cagtttgccg cgcaccgtct ggtgctggtg ggcgacgccg cgcataccat
    27901 tcacccgctg gcggggcagg gagtaaatct cggctttatg gatgctgcag agctgattgc
    27961 tgaactgaaa cggttgcatc gtcaggggaa agacatcggg cagtacattt acctgcgccg
    28021 gtatgagcgt agccgcaagc acagtgcggc gctgatgctg gctggtatgc agggattccg
    28081 cgatctgttt tccggtacca atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    28141 ggccgacacg cttcctggcg ttaaaccaca acttatccgc caggcaatgg gattaaacga
    28201 tttgcctgaa tggctgcgtt aaaaatttct cttctgctgt ttatttgata cccatcacac
    28261 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    28321 tccgttttcg cattatattt tctaatgtca ttattttttg atttagtgtt ttttgacatt
    28381 tttttagctc ttaatattgc cttattcaaa ttagctttct aatggcatca tctttatata
    28441 gaaactggtg tgttttttgg ttttttattc tatcgcgatt ttggcgtttt ttaaccataa
    28501 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    28561 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt tcgtggtgat
    28621 aatttcacca tgaaaagttg tcagccccgc ttattcaatg aggacaagat ggcacaacag
    28681 actcctttgt acgaacaaca cacgctttgc ggcgctcgca tggtggattt ccacggctgg
    28741 atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc atgcggtacg taccgatgcc
    28801 ggaatgtttg atgtgtcaca tatgaccatc gtcgatcttc gcggcagccg cacccgggag
    28861 tttctgcgtt atctgctggc gaacgatgtg gcgaagctca ccaaaagcgg caaagccctt
    28921 tactcgggga tgttgaatgc ctctggcggt gtgatagatg acctcatcgt ctactacttt
    28981 actgaagatt tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg
    29041 attacccaac acgctgaacc tttcggcatc gaaattaccg ttcgtgatga cctttccatg
    29101 attgccgtgc aagggccgaa tgcgcaggca aaagctgcca cactgtttaa tgacgcccag
    29161 cgtcaggcgg tggaagggat gaaaccgttc tttggcgtgc aggcgggcga tctgtttatt
    29221 gccaccactg gttataccgg tgaagcgggc tatgaaattg cgctgcccaa tgaaaaagcg
    29281 gccgatttct ggcgtgcgct ggtggaagcg ggtgttaagc catgtggctt gggcgcgcgt
    29341 gacacgctgc gtctggaagc gggcatgaat ctttatggtc aggagatgga cgaaactatt
    29401 tctcctttag ccgccaacat gggctggact atcgcctggg aaccggcaga tcgtgacttt
    29461 atcggtcgtg aagccctgga agtgcagcgt gagcatggta cagaaaaact ggttggtctg
    29521 gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg tacgctttac cgatgcgcag
    29581 ggcaaccagc atgaaggcat tatcaccagc ggtactttct ccccgacgct gggttacagc
    29641 attgcgctgg cgcgcgtgcc ggaaggtatt ggcgaaacgg cgattgtgca aattcgcaac
    29701 cgtgaaatgc cggttaaagt gacaaaacct gtttttgtgc gtaacggcaa agccgtcgcg
    29761 tgatttactt ttttggagat tgattgatga gcaacgtacc agcagaactg aaatacagca
    29821 aagaacacga atggctgcgt aaagaagccg acggcactta caccgttggt attaccgaac
    29881 atgctcagga gctgttaggc gatatggtgt ttgttgacct gccggaagtg ggcgcaacgg
    29941 ttagcgcggg cgatgactgc gcggttgccg aatcagtaaa agcggcgtca gacatttatg
    30001 cgccagtaag cggtgaaatc gtggcggtaa acgacgcact gagcgattcc ccggaactgg
    30061 tgaacagcga accgtatgca ggtggctgga tctttaaaat caaagccagc gatgaaagcg
    30121 aactggaatc actgctggat gcgaccgcat acgaagcatt gttagaagac gagtaacggc
    30181 tttattcctc ttctgcggga gaggatcagg gtgaggaaaa tttatgcctc accctcactc
    30241 tcttcgtaag gagagaggtt cacaattcac tgcacgtttc aggaaccatc gctcatgaca
    30301 cagacgttaa gccagcttga aaacagcggc gcttttattg aacgccatat cggaccggac
    30361 gccgcgcaac agcaagaaat gctgaatgcc gttggcgcac aatcgttaaa cgcgctgacc
    30421 ggccagattg taccgaaaga tattcagctt gcgaccccgc cgcaggttgg cgcaccggcg
    30481 accgaatacg ccgcactggc agaactcaag gctattgcca gtcgcaataa acgcttcacg
    30541 tcttacatcg gcatgggtta caccgccgtg cagctaccgc cggttatcct gcgtaacatg
    30601 ctggaaaatc cgggctggta taccgcgtac actccgtatc aacctgaagt ctcccagggc
    30661 cgccttgaag cactgctcaa cttccagcag gtaacgctgg atttgactgg actggatatg
    30721 gcctctgctt ctcttctgga cgaggccacc gctgccgccg aagcaatggc gatggcgaaa
    30781 cgcgtcagca aactgaaaaa tgccaaccgc ttcttcgtgg cttccgatgt gcatccgcaa
    30841 acgctggatg tggtccgtac tcgtgccgaa acctttggtt ttgaagtgat tgtcgatgac
    30901 gcgcaaaaag tgctcgacca tcaggacgtc ttcggcgtgc tgttacagca ggtaggcact
    30961 accggtgaaa ttcacgacta cactgcgctt attagcgaac tgaaatcacg caaaattgtg
    31021 gtcagcgttg ccgccgatat tatggcgctg gtgctgttaa ctgcgccggg taaacagggc
    31081 gcggatattg tttttggttc ggcgcaacgc ttcggcgtgc cgatgggcta cggtggccca
    31141 cacgcggcat tctttgcggc gaaagatgaa tacaaacgct caatgccggg ccgtattatc
    31201 ggtgtatcga aagatgcagc tggcaatacc gcgctgcgca tggcgatgca gactcgcgag
    31261 caacatatcc gccgtgagaa agcgaactcc aacatttgta cttcccaggt actgctggca
    31321 aacatcgcca gcctgtatgc cgtttatcac ggcccggttg gcctgaaacg tatcgctaac
    31381 cgcattcacc gtctgaccga tatcctggcg gcgggcctgc aacaaaaagg tctgaaactg
    31441 cgccatgcgc actatttcga caccttgtgt gtggaagtgg ccgacaaagc gggcgtactg
    31501 acgcgtgccg aagcggctga aatcaacctg cgtagcgata ttctgaacgc ggttgggatc
    31561 acccttgatg aaacaaccac gcgtgaaaac gtaatgcagc ttttcaacgt gctgctgggc
    31621 gataaccacg gcctggacat cgacacgctg gacaaagacg tggctcacga cagccgctct
    31681 atccagcctg cgatgctgcg cgacgacgaa atcctcaccc atccggtgtt taatcgctac
    31741 cacagcgaaa ccgaaatgat gcgctatatg cactcgctgg agcgtaaaga tctggcgctg
    31801 aatcaggcga tgatcccgct gggttcctgc accatgaaac tgaacgccgc cgccgagatg
    31861 atcccaatca cctggccgga atttgccgaa ctgcacccgt tttgtccgcc ggaacaggct
    31921 gaaggttatc agcagatgat tgcgcagctg gctgactggc tggtgaaact gaccggttac
    31981 gacgccgttt gtatgcagcc gaactccggc gcacagggcg aatacgcggg cctgctggcg
    32041 attcgtcatt atcatgaaag ccgcaacgaa gggcatcgcg atatctgcct gatcccggct
    32101 tctgcgcacg gaactaaccc cgcttctgca catatggcag gaatgcaggt ggtggttgtg
    32161 gcgtgtgata aaaacggcaa catcgatctg actgatctgc gcgcgaaagc ggaacaggcg
    32221 ggcgataacc tctcctgtat catggtgact tatccttcta cccacggcgt gtatgaagaa
    32281 acgatccgtg aagtgtgtga agtcgtgcat cagttcggcg gtcaggttta ccttgatggc
    32341 gcgaacatga acgcccaggt tggcatcacc tcgccgggct ttattggcgc ggacgtttcg
    32401 cacctcaacc tgcataaaac tttctgcatt ccgcacggcg gtggtggtcc gggtatggga
    32461 ccgatcggtg tgaaagcgca tctggcaccg tttgtaccgg gtcatagcgt ggtgcaaatc
    32521 gaaggcatgt taacccgtca gggcgcagtt tctgcggcac cgttcggtag cgcctctatc
    32581 ctgccaatca gctggatgta catccgcatg atgggcgcag aagggctgaa aaaagcaagc
    32641 caggtggcaa tcctcaacgc caactatatt gccagccgcc tgcaggatgc cttcccggtg
    32701 ctgtataccg gtcgcgacgg tcgcgtggcg cacgaatgta ttctcgatat tcgcccgctg
    32761 aaagaagaaa ccggcatcag cgagctggat attgccaagc gcctgatcga ctacggtttc
    32821 cacgcgccga cgatgtcgtt cccggtggcg ggtacgctga tggttgaacc gactgaatct
    32881 gaaagcaaag tggaactgga tcgctttatc gacgcgatgc tggctatccg cgcagaaatt
    32941 gaccaggtga aagccggtgt ctggccgctg gaagataacc cgctggtgaa cgcgccgcac
    33001 attcagagcg aactggtcgc cgagtgggcg catccgtaca gccgtgaagt tgcggtattc
    33061 ccggcaggtg tggcagacaa atactggccg acagtgaaac gtctggatga tgtttacggc
    33121 gaccgtaacc tgttctgctc ctgcgtaccg attagcgaat accagtaatt cactgattcg
    33181 actattttct aaaggcgctt cggcgccttt ttagtcagat gacaaagtac aaaagtgctc
    33241 agacagtccc ctcgcccctt tggggagagg gttagggtga ggggaacagg ccggcactgg
    33301 cgcgaatatt taccctcacc ccggccctct ccctgaaagg gcgaggggga aaagcgtgcc
    33361 aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg aattaatttt
    33421 tctcggaaaa acaaatggct atagcacttg tgactggtgg cagtcgcggc atcgggcggg
    33481 caactgcatt actgttggcg caagaagggt atacggtggc ggttaattat cagcaaaacc
    33541 tccacgcggc gcaggaagtg atgaacttaa taacgcaagc cggtggcaaa gcattcgtgc
    33601 tccaggcgga tatcagcgac gaaaatcagg tcgtcgagat gtttaccgca atcgatcagc
    33661 acgatgaacc gctagccgca ctggtcaata acgccgggat cttgtttacc cagtgcaccg
    33721 tagaaaacct taccgcagag cgaatcaacc gggtactttc caccaatgtg acgggacatt
    33781 ttctctgttg ccgcgaggcg gtaaaacgca tggcgctgaa aaatggcggt aatggcgggg
    33841 ctatcgtcaa cgtctcttca gtggcctcac ggctgggatc tccgggggaa tacgttgatt
    33901 atgcggcatc gaaaggggcg attgatacgt taaccacagg gctgtcgctg gaagtcgctg
    33961 cgcaaggaat tcgcgttaat ggcgtgcggc ccggtttttc tataccggaa tgcacgccag
    34021 cggtggcgaa cctggacgcg ttgatcgcgt taagtcgaac atccccatgc agcgcggtgg
    34081 gcaggcggaa gaggtcgcgc aggccatcgt ctggctgtta agtgataaag cctcttatgt
    34141 cacgggaagt tttatcgatt tggcgggcgg aaaataagat gggaaagtta tctgaccgga
    34201 tgcaacacaa ttggcgttgc atccggcaca tcagatagtc acagattctc gccgttgctg
    34261 gcaatcacct ctttgtacca gtcaaagctc ttcttacgtg aacgcgacat atcgccagta
    34321 ccgtcgtcat gtttattcac atagataaag ccgtagcgtt tgctgtactg cccggtagtg
    34381 aacgacacgc agtcgatgca gccccacggt gtgtagccca tcagatctac gccatcgtaa
    34441 gtcaccgctt ttttcatctc ttcaatatgg gcgcgcaggt agtcaatgcg gtagtcgtcg
    34501 ttgatgctgc catcttcttc cactttgtca taagcgccaa aaccgttttc gacaataaac
    34561 agcggcttct gataacgctc atacagttcg caaagtgcgt aacgcagacc caccggatca
    34621 atctgccagc cccagtcaga tgctttaaca tacgggtttg gtacgctgcc ttcaaaacca
    34681 gagatcgcat cgccggtgcc gccttcggcc ttcactgcat tggtcatgta atagctgaaa
    34741 ccaagataat cgcaggtgcc ttcacgcagc acatccagat cgccgtcttc cattttgatg
    34801 ttaaatccgc gacgctccca ctcgttcaac acataggacg ggtaatagcc gcgtagctgc
    34861 acatcggtaa agacgtagcg ttcgcgcatc gactcctgag cgaacatcac atcgtccggg
    34921 ttacaggagt aaggatagag cggcaccatc gccagcatac agccgacttt catctccggg
    34981 ttaatgcgac gcgcagcttt caccgccagg gcgctggcga caaactggtg atgcagcacc
    35041 tgatacatcg tctcttccgg gttttcatgc tcggtataca ccacgccgga gcagcagtaa
    35101 ccgaacagcg gtgcacgcca gttacgctgg ttgttaattt cgttgaaggt catccagtat
    35161 ttgactttgt gcttatagcg ttcaaatacc acttccgcga aacgtacaaa gaaatcaacc
    35221 actttacggt tggtccagct accgtattgc tgcaccagat gcagcggcat ttcaaagtgg
    35281 gagagggtga tcaccggttc gatgttgtat ttcagcagtt catcgaacat atcatcgtag
    35341 aacttcagcc cttcttcgtt tggctgagct tcatcgcctt ttggaaaaat gcgcgtccag
    35401 gcaatggatg tacgaaaaca tttgaagccc atttcggcaa atagcttgat gtcttcctta
    35461 tagtgaccat aaaaatcaac ggcttcatgg tttggatagt attttcctgg caagacttct
    35521 ttggtgattt cgcgcggcac gccgtgtgcg ccaccggtca gaacgtcaca aatgctcggc
    35581 ccttttccgc ctttgttcca gccgccttcg acctgatgag cggcaactgc gccgccccat
    35641 aagaaatctt tcggtaaggt gagttttttc actatcatag gctccagatg agtcaattct
    35701 tacaggaaat agtaccatgc agccaaacga catcactttt tttcaacgtt tccaggatga
    35761 cattctggct gggcgtaaaa ccatcaccat ccgcgacgag tctgaatcgc acttcaaaac
    35821 gggtgatgtg cttcgtgtcg gacgttttga agatgacggt tatttttgca cgattgaagt
    35881 caccgcaacc tcaaccgtaa cgctggatac gctgactgaa aaacatgcag agcaggaaaa
    35941 tatgaccctg actgaactga aaaaagtcat tgctgacatc tatcccggtc agacacaatt
    36001 ttatgtgatt gaatttaaat gtctttaaat tgaactcacg cctgttagtt gaaatttcgc
    36061 atttaatttc atgattttta ggagattagt ctcaaatcag aatttatttt agctaacagg
    36121 tgttcactgg aactattctc agttacgctg gagaggtata catgtgcgtg tacgtttctc
    36181 cggagtaagt tatggttcag aagcccctca ttaagcaggg atattcactg gcagaggaaa
    36241 tagccaacag cgtcagtcac ggcattgggt tggtgtttgg tatcgttggg ctggtgttgc
    36301 tactggttca ggcggtggat cttaatgcca gcgccacagc gataaccagc tacagcctct
    36361 atggcggcag tatgatccta ctgttcctcg cttcgacgct ctatcacgcc attcctcatc
    36421 aacgggcaaa aatgtggctg aagaaatttg accattgcgc tatttacctg ttgattgccg
    36481 gaacctacac gccgtttttg ctggtggggc tggattctcc gttagcgcgc gggttgatga
    36541 ttgttatctg gagcctggca ttgctgggta ttctgtttaa actgaccatc gcgcaccgat
    36601 tcaaaatttt atctctggtg acctatctgg cgatgggctg gctgtcgctg gtggtaattt
    36661 atgaaatggc agttaagctc gcggcgggca gcgttacctt actggcggta ggcggcgtgg
    36721 tttattcgct cggggtgatt ttctacgtct gcaaacgcat tccatacaac catgccatct
    36781 ggcacggctt cgtgctcggc ggtagtgtgt gccactttct ggcgatctat ttgtatattg
    36841 ggcaggcgta atgggtgtgc ctttgtaggc atgataagac gcgtcagcgt cgcatcaggc
    36901 atcggagcgc aaccgccgga tgcggcgtga acgccttatc cgacttacgg gcctcgcccc
    36961 tgttgtgata aaggtatcaa atcccaggca tgataagacg agtcagcgtc gcatcaggcg
    37021 ataaatgcct gatgcgataa agagatttac tcttcgagcg aatacggcag cggctcgata
    37081 tgcaatgtat tcgcatcgtc gcgtaagcgg aagatgctgt ccggttccat atcgttattc
    37141 atgacgactt gtaccacgac ctggccatct tccagtttta ccgcagccag caccgtaccg
    37201 gtgcgacgcc agttctcgcc cattttcagc tctaagtctt caccagcttc cggcagtcgg
    37261 ctggcactac ctgtcagcaa ccagagcgcg cgtttgttgg caccacggaa ttttgctcgc
    37321 gccaccatct cttgtccggt atagcagcct ttcttaaagc tgataccgcc cagcgcctgg
    37381 aggttggtcg cctgtgggat aaactgcccg ctgttggcgg catcaatcac cgggaaacca
    37441 gcttcaatgt ttaatgccag ccactgttgg ctattgttca gttccgcttc accgcgcagt
    37501 ttatcggtca gcatattggc agtagcttca tcggttacga tcaggaaacg ttctgccgga
    37561 tgttcaaacc atagcagggt agtcgcatct tctctgataa cctgcttttc cttcgaaggt
    37621 aatacactaa agaggtttgc cagcgcggcg cgcgcctgaa aaccggcaac accaagcagc
    37681 acacgctcgt cgtctggcgc gatggtcact ttagagaata ccgcatattt tttcagttca
    37741 gtcagctgcg gttcacgcac gctgcgccgt tcaatccacg caaagccatc gccgtcgcgg
    37801 aacagacgta aattgctcca cattttacct ttggcgtcgc aatgggcggc cagcaggtgc
    37861 tgatcttctg tcatttggct aacgtctgct gtcacctgac cctgcatata tttttcgctg
    37921 tccgcgccag taatggtggc aagcgcccag tcatcaagcg tcatcagtgt cagtggcaaa
    37981 cgggcagaag ccgtaggctg acggggcgga aaaggtgtaa aagccataat agagtcctga
    38041 gttgcttaac ggcgtattaa tgactaatgg taaaagagct atgaggcaat gcaagtgctt
    38101 tcactacgcg atctgtgcct tctgcgaggc gacttccaaa aaaatgaagc ccttgaattg
    38161 ttgtgaatta atttcgcgat cgcgattgtg tttactgata ttcccgtaaa agaagtcgaa
    38221 aaacacgtta caatagcggg gtactggatt ttcgtgagac aggaagacga tggacattaa
    38281 caacaaagcc cgcattcatt gggcatgccg ccgtggtatg cgcgaactcg atatttcaat
    38341 catgccgttt ttcgaacatg agtatgacag cttaagcgat gacgaaaaac gcatctttat
    38401 tcgtctgctg gaatgtgacg atccggacct gtttaactgg ctgatgaatc acggtaaacc
    38461 ggccgatgca gaactggaaa tgatggtccg actcatccag acacggaacc gggaacgtgg
    38521 tcctgtggca atctgatttg cgcgtctcct ggcgcgcgca gtggctttcc ttgctgattc
    38581 atgggctggt tgccgctgtt attttactca tgccctggcc gctcagttac accccgttat
    38641 ggatggtgtt actttcgctg gtggtgtttg attgcgttcg cagccagcgg cgcattaatg
    38701 ctcgccaggg ggaaattcgc ctgttgatgg acgggcgttt gcgttggcaa gggcaggagt
    38761 ggagcatcgt caaagcgccg tggatgatta agagcggcat gatgctgcgt ttacgttctg
    38821 atagcggtaa acggcaacat ttatggctgg cagccgacag catggacgaa gccgaatggc
    38881 gggatttacg gcggattttg ttgcaacaag agacgcaaag ataaagatga cggcaaagca
    38941 tatgcgctaa gccgtcagtg agatcaggcg taatgctctg ccatttcgtt gaggatttgc
    39001 tcgcaccagc tctgaatacg ctcgtcgcta aggtcatact ggttagtttc atccagcgcc
    39061 agacccacga acagttgccc gtcagcaatc accggtttcg ggctggtaaa ttcatatcct
    39121 tccgttggcc agtagccgac gaacttcacg cctttggtag aaagtttgtc atgcagtatc
    39181 cctagcgcat cgaggaacca ttcaccgtac ccgagttgat cgccaagccc atacagcgca
    39241 acaattttac cttcgaggtt caggtcgtcg agctgatccc agacggcttc ccagtcttcc
    39301 tggatttcgc caaaatccca ggtcgggata cccagaatga gcacatcgta ctgctccatt
    39361 aatttcgggg agtcgtcctt gaggttatgt aaggtcacca gttctgggcc gataatatcg
    39421 cggatttttt ctgccgccat ttcggtgtaa caggtgctgg aaccgtaaaa aagacccata
    39481 ttcataacgt aagcttctcg attctgctgt ggcttttgcg gctagtgtat cagaatcgct
    39541 ttagtcacgg cacaataagg cataatggat caaatgagtg accataaggg gcgcaagtga
    39601 aacaggatct ggcacgcatc gagcagtttc ttgatgctct gtggctggaa aaaaatctgg
    39661 ctgaaaatac gttgaacgct taccgtcgcg atctgtcaat gatggtggag tggttgcatc
    39721 accgcgggtt gacgctggcg acggcgcaaa gtgacgattt gcaggcatta ctggcagaac
    39781 ggctggaggg cgggtataaa gccaccagct cagcgcgttt gctgagtgca gtgcgccgat
    39841 tgttccagta tctttatcgc gaaaagtttc gtgaagacga tcccagtgcg catctcgctt
    39901 caccgaaatt gccacagcgt ttgccaaaag atttaagcga agcgcaggtc gaacgtttat
    39961 tacaggcacc attaattgat cagccactgg agctacgcga taaagccatg cttgaagtgt
    40021 tgtatgctac cgggctgcgt gtctctgaac tggtcgggct gacaatgagt gatatcagcc
    40081 tacgtcaggg cgtagtgcgg gtcattggta aaggcaacaa agagcgcctg gtgccgttag
    40141 gtgaagaggc ggtttactgg ctggaaacct atctggaaca tgggcgtccg tggctgttga
    40201 atggtgtgtc aattgacgtg ttgtttccca gccagcgtgc gcagcagatg acgcgacaga
    40261 ccttctggca ccgcattaaa cattatgccg tgctggcggg tatcgacagc gaaaagctgt
    40321 caccgcatgt gttgcgtcac gcttttgcca ctcatttatt aaatcatggt gcggatttac
    40381 gcgtggtgca gatgctactg ggccacagcg atctctccac cacgcaaatt tatacgcatg
    40441 tcgctaccga gcgtctgcgg caacttcatc aacagcatca cccgcgggcg tgatgtctga
    40501 aaagaacggg aagatttatg aagaaaggtt ttatgttgtt tactttgtta gcggcgtttt
    40561 caggctttgt tcaggctgat gacgcggcaa ttcaacaaac gttagccaaa atgggcatca
    40621 aaagcagcga tattcagccc gcgcctgtag ctggcatgaa gacagttctg actaacagcg
    40681 gcgtgttgta catcaccgat gatggtaaac atatcattca ggggccaatg tatgacgtta
    40741 gtggcacggc tccggtcaat gtcaccaata agatgctgtt aaagcagttg aatgcgcttg
    40801 aaaaagagat gatcgtttat aaagcgccgc aggaaaaaca cgtcatcacc gtgtttactg
    40861 atattacctg tggttactgc cacaaactgc atgagcaaat ggcagactac aacgcgctgg
    40921 ggatcaccgt gcgttatctt gctttcccgc gccaggggct ggacagcgat gcagagaaag
    40981 aaatgaaagc tatctggtgt gcgaaagata aaaacaaagc gtttgatgat gtgatggcag
    41041 gtaaaagcgt cgcaccagcc agttgcgacg tggatattgc cgaccattac gcacttggcg
    41101 tccagcttgg cgttagcggt actccggcag ttgtgctgag caatggcaca cttgttccgg
    41161 gttaccagcc gccgaaagac atgaaagagt tcctcgacga acaccaaaaa atgaccagcg
    41221 gtaaataatt cgcgtgaaac aacagataca acttcgtcgc cgtgaagtcg atgaaacggc
    41281 agacttgccc gctgaattgc ctcccttgct gcgccgttta tacgccagcc gaggagtacg
    41341 cagtgcgcaa gaactggaac gcagtgttaa aggtatgttg ccctggcagc aactgagcgg
    41401 cgtcgaaaag gccgttgaga tcctttacaa cgcttttcgc gaaggaacgc ggattattgt
    41461 ggtcggtgat ttcgacgccg acggcgcgac cagcacggct ctaagcgtgc tggcgatgcg
    41521 ctcgcttggt tgcagcaata tcgactacct ggtaccaaac cgtttcgaag acggttacgg
    41581 cttaagcccg gaagtggtcg atcaggccca tgcccgtggc gcgcagttaa ttgtcacggt
    41641 ggataacggt atttcctccc atgcgggggt tgagcacgct cgctcgttgg gcatcccggt
    41701 tattgttacc gatcaccatt tgccaggcga cacattaccc gcagcggaag cgatcattaa
    41761 ccctaacttg cgcgactgta atttcccgtc gaaatcactg gcaggcgtgg gtgtggcgtt
    41821 ttatctgatg ctggcgctgc gcaccttttt gcgcgatcag ggctggtttg atgagcgtaa
    41881 catcgcaatt cctaacctgg cagaactgct ggatctggtc gcgctgggga cagtggcgga
    41941 cgtcgtgccg ctggacgcta ataatcgcat tctgacctgg caggggatga gtcgcatccg
    42001 agccggaaag tgccgtccgg ggattaaagc gctgcttgaa gtggcaaacc gtgatgcaca
    42061 aaaactcgcc gccagcgatt taggttttgc gctggggcca cgtctcaatg ctgccggacg
    42121 actggacgat atgtccgtcg gtgtggcgct gttgttgtgc gacaacatcg gcgaagcgcg
    42181 cgtgctggca aatgaactcg atgcgctaaa ccagacgcga aaagagatcg aacaaggaat
    42241 gcaaattgaa gccctgaccc tgtgcgagaa actggagcgc agccgtgaca cgctacccgg
    42301 cgggctggca atgtatcacc ccgaatggca tcagggcgtt gtcggtattc tggcttcgcg
    42361 catcaaagag cgttttcacc gtccggttat cgcgtttgcg ccagcaggtg acggtacgct
    42421 gaaaggttcc ggtcgctcca ttcaggggct gcatatgcgt gatgcgctgg agcgattaga
    42481 cacactctac cctggcatga tgctgaagtt tggcggtcat gcgatggcgg cgggtttgtc
    42541 gctggaagag gataaattca aactctttca acaacggttt ggcgaactgg ttactgagtg
    42601 gctggaccct tcgctattgc aaggcgaagt ggtatcagac ggtccgttaa gcccggccga
    42661 aatgaccatg gaagtggcgc agctgctgcg cgatgctggc ccgtgggggc agatgttccc
    42721 ggagccgctg tttgacggtc atttccgtct gctgcaacag cggctggtgg gcgaacgtca
    42781 tttgaaggtg atggtcgaac cggtcggcgg cggtccactg ctggatggta ttgcttttaa
    42841 tgtcgatacc gccctctggc cggataacgg cgtgcgcgaa gtgcaactgg cttataagct
    42901 cgatatcaac gagtttcgcg gcaaccgcag cctgcaaatt atcatcgaca atatctggcc
    42961 aatttagcgt catcttctct ataaaaaaga gcgtggattg ggtacaatcc cgctcttatc
    43021 accgcatttt gactagctca ataaaagaaa tcagaccatg tttgaaatta atccggtaaa
    43081 taatcgcatt caggacctca cggaacgctc cgacgttctt agggggtatc tttgactacg
    43141 acgccaagaa agagcgtctg gaagaagtaa acgccgagct ggaacagccg gatgtctgga
    43201 acgaacccga acgcgcacag gcgctgggta aagagcgttc ctccctcgaa gccgttgtcg
    43261 acaccctcga ccaaatgaaa caggggctgg aagatgtttc tggtctgctg gaactggctg
    43321 tagaagctga cgacgaagaa acctttaacg aagccgttgc tgaactcgac gccctggaag
    43381 aaaaactagc gcagcttgag ttccgccgta tgttctctgg cgaatatgac agcgccgact
    43441 gctacctcga tattcaggcg gggtctggcg gtacggaagc acaggactgg gcgagcatgc
    43501 ttgagcgtat gtatctgcgc tgggcagaat cgcgtggttt caaaactgaa atcatcgaag
    43561 agtcggaagg tgaagtggcg ggtattaaat ccgtgactat caaaatctcc ggcgattacg
    43621 cttacggctg gctgcgtaca gaaaccggcg ttcaccgcct ggtgcgtaaa agcccgtttg
    43681 actccggcgg tcgtcgccac acgtcgttca gctccgcgtt tgtttatccg gaagttgatg
    43741 atgatattga tatcgaaatc aacccggcgg atctgcgcat tgacgtttat cgcgcgtccg
    43801 gcgcgggcgg tcagcacgtt aaccgtaccg aatctgcggt gcgtattacc cacatcccga
    43861 ccgggatcgt gacccagtgc cagaacgacc gttcccagca caagaacaaa gaccaggcca
    43921 tgaagcagat gaaagcgaag ctttatgaac tggagatgca gaagaaaaat gccgagaaac
    43981 aggcgatgga agataacaaa tccgatatcg gctggggcag ccagattcgt tcttatgtcc
    44041 ttgatgactc ccgcattaaa gatctgcgca ctggggtaga aacccgcaac acgcaggccg
    44101 tgctggacgg cagcctggat caatttatcg aagcaagttt gaaagcaggg ttatgaggaa
    44161 ccaacatgtc tgaacaacac gcacagggcg ctgacgcggt agtcgatctt aacaatgaac
    44221 tgaaaacgcg tcgtgagaag ctggcgaacc tgcgcgagca ggggattgcc ttcccgaacg
    44281 atttccgtcg cgatcatacc tctgaccaat tgcacgcaga attcgacggt aaagagaacg
    44341 aagaactgga agcgctgaac atcgaagtcg ccgttgctgg ccgcatgatg acccgccgta
    44401 ttatgggtaa agcgtctttc gttaccctgc aggatgtggg tggccgcatt cagctgtacg
    44461 ttgcccgtga cgatctgccg gaaggcgttt acaacgagca gttcaaaaaa tgggacctcg
    44521 gcgacatcct cggcgcgaaa ggtaaactgt tcaaaaccaa aaccggcgaa ctgtctatcc
    44581 actgtaccga gctgcgtctg ctgaccaaag cactgcgtcc gctgccggat aaattccacg
    44641 gcttgcagga tcaggaagcg cgctatcgtc agcgttatct ggatctcatc tctaacgatg
    44701 aatcccgtaa cacctttaaa gtgcgctcgc agatcctctc tggtattcgc cagttcatgg
    44761 tgaaccgcgg ctttatggaa gttgaaacgc cgatgatgca ggtgatccct ggcggtgccg
    44821 ctgcgcgtcc gtttatcacc caccataacg cgctggatct cgacatgtac ctgcgtatcg
    44881 cgccggaact gtacctcaag cgtctggtgg ttggtggctt cgagcgtgta ttcgaaatca
    44941 accgtaactt ccgtaacgaa ggtatctccg tacgtcataa cccagagttc accatgatgg
    45001 aactctatat ggcttacgca gattacaaag atctgattga gctgaccgaa tcgctgttcc
    45061 gtactctggc acagaatatt ctcggtaaga cggaagtgac ctacggtgac gtgacgctgg
    45121 acttcggtaa accgttcgaa aaactgacca tgcgtgaagc gatcaagaaa tatcgcccgg
    45181 aaaccgacat ggcggatctg gacaacttcg actctgcgaa agcgattgct gaatctatcg
    45241 gtatccacgt tgagaagagc tggggtctgg gccgtatcgt taccgagatc ttcgaagaag
    45301 tggcagaagc acatctgatt cagccgacct tcattactga atatccggca gaagtttctc
    45361 cgctggcgcg tcgtaacgac attaacccgg aaatcacaga ccgctttgag ttcttcatcg
    45421 gtggtcgtga aatcggtaac ggctttagcg agctgaatga cgcggaagat caggcgcaac
    45481 gcttcctgga tcaggttgcc gcgaaagacg caggtgacga cgaagcgatg ttctacgatg
    45541 aagattacgt taccgcactg gaacatggct taccgccgac agcaggtctg ggaattggta
    45601 tcgaccgtat ggtaatgctg ttcaccaaca gccataccat ccgcgacgtt attctgttcc
    45661 cggcgatgcg tccggtaaaa taagcattac gttatgctca taaccccggc aaatgtcggg
    45721 gttttttatt taagctgggt aaatgcagat aatcgtattc tggcttcgcg atttgtcgcc
    45781 tgcatcacca tccacggact gaacgcccac ggcgtggcat caataccgcg taatacatca
    45841 gctaaatcac accattgata atccatcact tcatcatcgt tgatctgtaa cgcactggtg
    45901 gtgcgtgcgg caaataccgg acacacttca ttttccacaa tgccattcgg atcggtggcg
    45961 cgatagcgaa agtcaggata gatagattca ggaggcgtaa tttccacgcc aagctcataa
    46021 cggcaacggc ggatcaccgc gtcttcgttg ctttctccca gttgtgggtg cccacaaacc
    46081 gagttagtcc acacgccagg ccatgctttt ttgctcagag cgcgacgggt aactaataat
    46141 tgtcctttgg cattaaacag ccaactggag aacgcgagat gtaagcgggt gtctgccgtg
    46201 tgtgcggcat acttttccag cgtacccgtg ggaactccct gtgcattcaa taaaatgacg
    46261 tgttccgttt gcataatttc tcacatgtaa ttctgatcac tcatcattat agataattga
    46321 agagtgaaat gattaaccaa gtgagcgttt atcgacaacc gcccgttttg agcggatgtc
    46381 gacaggtaaa aaccatttaa aaaagtagca tcatatattt gaagcgactt tcaatggcca
    46441 ttgcaacaca gtcagaaatg acaggaatag agagagtaaa aagcaacagc agactgaata
    46501 tagcgcgcat taaaaaactc atattattct ccatgctcat ttttcttcag cttgcgcatc
    46561 agaagccaca tacgtactgt cctgactttt aaatctttat cagagacgga ttccttcacc
    46621 aggtcagcat gatgctgata gccattaaag aagacgttca gcacaacggc gctaagggtt
    46681 gcgagcataa tgccgctatg tagcagcggt tgcagtacgg ccggtaactt agaaaagaaa
    46741 tcgtgagaga gcgtcggagt catgccaacg ccgagactga tcgccacaat atagaggttg
    46801 taacggttgg tggtgtagtt acagcgcgac agaattcgaa tccctgtcgc cagtaccatg
    46861 ccgaacatca ccagcccagc gccgcccagc acaaattgcg gaatggaagc taccagcacc
    46921 gccattttgg gcaccatgcc aaacaggatt aatataattc ccgaagaaat acacacccag
    46981 cggctatgaa cgcgcgtcac gctaaccagg ccaacgtttt gagaaaatga cgtgtggggg
    47041 aagctgttaa acgttccgcc tatcattgtt cctacgccat cgacacgcag cccgcgaata
    47101 atatcgtgcg aagagagttt acgaccgact atttcaccca gtgccaggaa catccccatc
    47161 gactcgataa acacgatgat taacacggca gtcatggtca gaatggacac gggatcgaaa
    47221 atcggcatac caaacgacat cggggtgaca atcgcaaacc acgaggcatc atgtagcccg
    47281 gataaattga cttcattcat catccacgaa agtaaaaagc caaatacaat ccccagtaat
    47341 acggcgacgt tggacataaa tcctttcgca tagcgagtaa tgagcaagat aaaaattaag
    47401 acggcaaagg agatacctaa ataaacggga ttaccatatt gcggattccc tttacctccg
    47461 gcggcccagt caatacccac ctgaatgatg ctaagcccga tagaagtaat aaccacaccg
    47521 gtaaccagtg gcggaaataa aggcatcaag cgaccgataa gtggcgctaa taatgtggtg
    47581 ataaaacccg cggcaatagt ggcaccaaat atccccagca ggccgatatc cgggttcatc
    47641 ccaatggcta tcattggtgt tacagcagca aaggtcaccg acataatcac cggcaggcgg
    47701 atccccataa agcggccgat accgatacat tgcaataatg tgacgatccc gcagcaaaag
    47761 agatccgagc taatgagcat cgcaatagct tcttttgaga ggcccagtcg gtcaccaatc
    47821 ataagaggaa cagcgactgc acctgcgtac atgacaagga cgtgttgcag accgagtatg
    47881 atcagctttc ctggtgataa tatgcggtca acctcatcag ttgggcggtc ttgcccagaa
    47941 gatgagggaa gttgggaatc tatggcgctc atcagtgttt ctcctttcgc ccgccagagt
    48001 aaaaagcaac cgtttacgtt aatgaaactg tctaaacaca tgctcgtgga gtgccagttt
    48061 ttgcgaggaa gggaagttag agacaggaat tgcggaggca atcacataat cgtgcttaaa
    48121 ccacgaaaat gacaagagaa cctgacaatg gaattatctg taattttctc atattgatat
    48181 ttcattaatc attctggcat gtcgcactct cgcatttaat cgtttttatc tggatagcgc
    48241 tcttttgatc ggcaatgttg aaataatgaa agcggcatag tttgaggggc aataatttga
    48301 actcagtcgc aaaattataa ttaaaacggt catgctacct aatatatgcc cgtttattgc
    48361 atcgcgaaat ttgagaagac ataactcatt aaatgaaagg gatgcaaatg aataagttta
    48421 tcgctgctga agctgcggaa tgtataggct gtcatgcttg tgaaattgcc tgtgcggtgg
    48481 cacacaatca agaaaactgg ccgctgagtc acagtgactt tcgaccgcgt atccacgttg
    48541 tagggaaagg ccaggctgcg aatccggtgg cctgccatca ctgcaacaat gccccttgcg
    48601 ttacggcttg tccggttaat gctctgactt tccagtccga tagcgtacaa ctggacgagc
    48661 aaaaatgtat tggttgtaaa agatgcgcaa tcgcttgccc ctttggcgtc gttgagatgg
    48721 tcgatacgat tgcgcagaaa tgcgaccttt gtaaccagcg cagttccggc acgcaagcct
    48781 gtattgaagt ttgcccaaca caggcgttac gactgatgga cgataaaggg ttacagcaga
    48841 taaaggtggc ccgccagcgc aaaacggcag caggaaatgc gtcatcagac gctcagccat
    48901 ctcgcagtgc agcgttgctc cccgttaact cgcgtaaagg cgcagataaa atttcagcaa
    48961 gtgaacggaa aaaccacttt ggcgaaatct attgcgggct ggatccacaa caagcgactt
    49021 atgagagtga ccgctgtgtt tattgtgccg aaaaagctaa ctgcaactgg cattgcccgc
    49081 tgcataacgc tattccggat tacatccgtc tggtacagga gggaaagatt attgaagcgg
    49141 cagaactttg ccaccagacc agttccttac cagaaatctg cggcagggta tgcccacagg
    49201 accgtctttg tgaaggcgca tgtactttga aagatcactc tggcgcagtc tctatcggta
    49261 atctggaacg ctacatcacc gataccgcgc tggcgatggg ctggcgtccc gatgtcagca
    49321 aagttgttcc ccgtatcgag aaagtggcgg tgattggcgc tgggcctgca gggttagggt
    49381 gtgctgatat tctggcgcgc gcaggagttc aggtcgatgt ctttgatcgc catccagaaa
    49441 ttggcggtat gctgactttt ggcattcctc ctttcaaact cgataaaacg gtattaagcc
    49501 agcggcgaga gatattcacc gcaatgggaa tcgactttca tcttaactgt gaaattggcc
    49561 gcgatattac ctttagcgat ttaacttctg aatatgatgc agttttcatc ggcgtgggga
    49621 cttacgggat gatgcgagca gatctgccgc atgaagatgc gcccggtgtc attcaggctc
    49681 taccgttcct gactgcccat acccgccagc tcatgggatt gccggagtct gaagagtatc
    49741 cgctgacgga cgtggaaggt aagcgagtcg tggtattggg cggtggcgat acgacaatgg
    49801 attgtttgcg gacttccatt cgcctcaatg ctgccagcgt gacctgcgcg tatcgtcgtg
    49861 atgaagtcag tatgccgggc tcgcgcaaag aggtggtcaa tgcgcgcgag gaaggtgtcg
    49921 agtttcaatt caatgttcag ccgcaatata tcgcttgtga cgaagatggg cgtttaactg
    49981 cagtgggcct gattcgtacc gccatgggtg agctggggcc ggatggtcgc cgtcgtcctc
    50041 gtccggttgc gggttcagag tttgaattgc ccgccgatgt tctcattatg gcctttggtt
    50101 tccaggcgca tgccatgccg tggttgcagg gcagcggaat taaactcgat aaatggggcc
    50161 tgattcaaac cggcgacgtt gggtatttac ctacccagac gcatctgaaa aaagtctttg
    50221 ctggtgggga tgcagttcat ggcgcggatc tggttgtcac tgccatggcc gcaggaaggc
    50281 aggcggcgcg cgatatgtta actctgtttg atacgaaggc atcgtgatga aatcgttaat
    50341 tatcgttaat ccggctgact gtattggctg ccgtacttgt gaagtggcct gtgtggtcgc
    50401 tcacccttca gaacaggagt tgaatgccga tatcttttta ccccggctga aggtacagcg
    50461 actggactgc atcagtgcgc cggtgatgtg ccaccaatgt gaaaacgccc cttgtgttgg
    50521 cgcttgcccc gtgggggcgc tgacgatggg cgagcaggtg gtgcagacca attctgcccg
    50581 ttgtattggc tgtcagagtt gcgtcagtgc gtgcccgttt ggaatgatca ccattcagtc
    50641 gttgctgggg gatacccggc aataaatcgt gaaatgcgat ctctgtgaac agcgggaaga
    50701 ggggcctgcc tgcgttgaat cttgcccaac acaggcgctg cagttgctta ctgaaagaga
    50761 actcaggcga gtccgccagc agcgtattgt tgccagcggt gagaatcctc tctgaaagaa
    50821 atctccgcgc tgaaaggcgc ggagaatcta ttagattgcc caaccacctg cgtaaaatgc
    50881 gaccagtgcg gcagtaatga tcaccgtacc aatattcagc ttttgccatt cgcgtgcaaa
    50941 gacgcggcct acgaccagtg tcacaaagcc cagcataata ccggtaacga tattacaagt
    51001 cagaacgatg aacacggcac acaccaggcc agccatagcg tcaataaaat cattgaaatc
    51061 cagcttcgag acgttactta acatcagcaa acctacgtac atcagagcgg gtgcagtggc
    51121 gtaaccaggg atcaaaaatg acaacggtga taagaacaaa atcagcagga ataacgcccc
    51181 cactacggtt gcggtcaacc ctgttttacc cccggcggcg gttcctgccg ctgattcgat
    51241 ataaaccgct gcgggcgctg cgcccaccag gccggagaat attgaactta ctgagtcact
    51301 ggtcagggct ttgccgccgt tgatgatctg gttgtcttta tccaacaaat tcgcctgacc
    51361 ggcgacggca cggatggtgc cagtagcgtc gaacactgcg gtcatcacca atgccagtac
    51421 actcggaagt acagttggct ggagtgcgcc cataatatcg aggctgaaaa tcagagactt
    51481 accatcttcg ccagtcaggc ttggcatcgc caccagaccg tggtacttca ccgctggatc
    51541 aaagattaag ccgatgatcg aaattgcaat aatcaccaac aagatcccgc cgggtacgcg
    51601 acacttctcc agaccgaaga tgaccgccag tcccagcaag ctcatcatca ccgggaagga
    51661 ggtaaacgca ccgagcgcca ctggcaagcc ttcaatcggg tttttgataa ccattcccac
    51721 accgttagca gcaatcagca gcagaaacag gccgataccg atacctgtac cgtgagcgat
    51781 ccccatcggt aaattacgta agatccaggt acgcacaccg gttacggaaa tggcggtgaa
    51841 gatgacgccc atcagaaata ccgcgcccag tgcgacagga acgctaattt gttgcccgag
    51901 taccagactg aatgcggtaa acgccgtcaa ggaaatcgcg caaccaatcg ccattggcaa
    51961 attggcccat aatcccatca gcaacgagcc gaagcccgcg accagacagg tggcaacaaa
    52021 cacagctgcg ggaggaaaac ctgctttgcc cagcattccc ggaacgacga taacggaata
    52081 aaccatggcc agaaaggtcg ttaagccagc cagtacttcc tgacgaacgg tactgccacg
    52141 agcggtaatt ttaaaataat tatccagcgc gccctgtggc tttggtgcgt ccggtgtttg
    52201 taggatgtct ccagacataa tgatgtcctc acgaggatgt aaaagtttta ttattagttg
    52261 cgttcgtaca ccagacgacc atcaacgtag gtacggtaga tcgaacggtc atcgcccaac
    52321 gtcatcatca cgaacaattt gtcgactaaa gaaacagagt tgtcatagcg cagctgctgt
    52381 agcggagtgg cggtgggttc catcaccacg aaatcagcct ctttgccagg taaaaagttg
    52441 ccaatcaaat cgtcaaggcc cagagatttc gctccgccga gcgtggccag gtaaaacgct
    52501 tcatatgccg agaggcgata gccttgtaat tgcaacactt tgtaggcttc gttcagcgtt
    52561 tgcagcatgt tgaaagtggt tccggcaccg atatccgttc ccatgcccac tttgactttc
    52621 ttctgccatg cttttttcaa gttgaataag ccgctgccga ggtaaaggtt ggaggtcgga
    52681 cagaaagcaa tgctggattt ggtttcgctg agacgatccc actctttttc ttcaagatgg
    52741 acgcagtgag caaagacaca gtttttaccg gtcaggccgt actgatggta aacatccaga
    52801 taaccatcat ggtcaggata aagcgagttc acccaggcaa tttcatcttt gttttcacag
    52861 agatgggtat gtacccacgt atccggatat tcctctttca ggcgttgcgc catcgccatc
    52921 tgttcaggag aagaagtagg ggcgaagcgt ggtgtaatcg catatagcag acgaccattt
    52981 ttgtgccagc gttcaatcag ttctttgctt tggtgatagc tgctttcggc agtgtcgagc
    53041 agataatccg gtgcgttacg gtccatcatc accttaccgg caatcatacg catattgata
    53101 tgactggcgg cttcaaacag cgcatcaacg gattgcggat gaacagtgcc aaacaccagc
    53161 gccgtggtgg ttccgttacg taaaagctgc ttgatgaaga acgccgacat ttcgcgggcg
    53221 tactctaaat cctcataacg ccgttcagta gggaaggtgt gtttattcaa ccactccagc
    53281 aattgctcac cataggcccc caccatttca ctttgcggat aatggatatg tgtatcgaca
    53341 aagcccggta ctatcagttt gccgcgatag tcgcgcacgc gaatggtgtc aggaatttga
    53401 tgctttccgt tttcccattc gccaaaccat tccacttttc cctgtttaat gagtaataaa
    53461 ccatcctcaa taaaccgcag cgcagaggca atctcttccg ggttatcgac cgtacgggtg
    53521 acatcaataa aactgcctcg taccgctttt aacgtgtgtt ctcctgacat cattgactcc
    53581 tttactgtta atccatctct tccgctgagg taataccagg cagaacgttt tcctgtcggt
    53641 agccaccagg gaggataata tttaataaaa tcgcagttaa cccgccagca caaatagggt
    53701 tttcaactaa tacataaata gaggctggca atattttaaa aatttcggga tcgtaggaga
    53761 cgccaagccc taaacctaat gaagtggcga caataagtgt ttcacgccgc tttaatccgt
    53821 tggtgatgat gatgcgaatc ccagcgatgg caatcatgga aaacatcaac gtcattgcgc
    53881 ctcccagaac tgccgaggga atggtcgtga agaagccgcc aatcatcgga aataagccga
    53941 ggataaccag cattaccgcg atggttcgcc cgacataacg tgaagcgacg ccagtcatct
    54001 gaataacccc attattttgc gcaaacgtgg ttaatggtaa tgaaccaaca gcggaggcga
    54061 taacagaaac tagaccatct gccagcacgc cgcctttcag tcgggactga tactcttccc
    54121 cctgaatggg gcggcgggaa accattgccg tggcggtgat atcgccgact gcttccagca
    54181 cgctaagcag ataaatcgtg ccaaccacca ggaactgatg gaagctaaaa ctaaagccgt
    54241 atttgaacgg atgcgggatg gtgattaacg gcaaattgcg catactgctg aaatccacca
    54301 tgcccaggca taacgacgcg atataaccga cgcatagccc aatggcgatc cctcccatgc
    54361 gtagcaacgg actgcgacag cagttaaagc cgatcaccac aatcaaaacc aacaaaccaa
    54421 cgccgagatg ttcgtaattg ccgaacgtac cgctgctttt ggcagcaaat cctccaccaa
    54481 aatcgataat gccgacttta atcaggctta agccgatcat cagtaccaca ataccgctga
    54541 cggtaggcgt aataacccga cgtaaatagg gcaagataaa tgaagatccg acaaccagaa
    54601 atgcgccaac gaaggagacg ccgagaagcg acgacatgat taactcttcg tgaaaaccgt
    54661 cgcttttcat gctgctgccc agcgcaatca tgaccgtaac aaatgaaaaa ttgactgact
    54721 gaattgagag taggccagaa ccgacgatgc cgtagcggtt tacttgtaac caggtaccaa
    54781 taccagaggc gatcatcgcc atagaaacaa gataggcggt tgtttcagcg gaaagctgta
    54841 aggccgcacc cacgattaac gcgggggtta ccatcggaac gaaaattgcc aacagatggg
    54901 taatggcacc aacgagagcc tgatgaaagg gagggcgatc ctccagttca aatataaggt
    54961 cagaacctgc atggtttata tcagacatcc cttcactcct tttaaagtaa atttgatcat
    55021 ctgaattagt aaaataaatt cccacgaatt tggggcgcag gacgccttga gaacggctta
    55081 tctcattttc gcagtcacta tttagtcatc gttcaaacag agaaacaggc cgccgaagcg
    55141 gccatttatt atttatattt tttccagcgc agtgagtatt ttctccggtg tgaaatgcca
    55201 ttcgcgtaac cagatgccgc aggcatcgtg aattgcggta gcaatcgccg gagctgcgcc
    55261 atttacaccg atttccgaga tcgatttcgc cccgaacggg ccgactttat cgtcgctcgg
    55321 caccagcaca gcgcggaaat cacgcggaat gtcaccaatt ttcggtgcac cgtaactgcg
    55381 taaatcacgc gttaacgggt gaccttcggc gtcataaatg atctcttcgc tcatactgtg
    55441 gccgatagct cgcagggtgg caccgtagat ttgtcccaac gctaactctg gattgaccgg
    55501 tgtaccgcag tccagcaagg cgtagaattt atccaggctg atttcacccg tacgcgtgtt
    55561 gacggcaacc tcagcgaagt ttgcgccata cgggaaggcg aaatcaggcg tgatataact
    55621 gccagttccc accagtgaac caaagccggt gccggtttcg cctttatggg caatatcccc
    55681 gaaactaact tcgcctttct tgccgcgcac gacgcccggc gttgctagtt gaacatctgc
    55741 cactggctca cccaacattt gcgcgccgtg gaacagaatt ttctcccgca gattttccgc
    55801 tgctaaacgc gccgcgttac ccgagaagca agtaccggac gaggcatatg cgcctttatc
    55861 aaacaacgca tgatcggtat caccggagat aacatgcacg tcctgtggtg ggcagtgcag
    55921 cacttctgct gccagtttcg tcactacagt atccagacca gtaccaatat ccgcaccgcc
    55981 agaatgaacg ataaaggtgc cgtccgactc cagtttgatc atgcagttag cctgatcgat
    56041 atccgggata cccgatttct gcatgataat ggcgacgccg cgaccgatgt gccagtcacc
    56101 attttgcggt ttcggtgaag accattggat catctcgcga ccctgacgca ggatctcttc
    56161 cagtgcgcag ctggcggcgg aaggaacgga ggttggcgct ttaccttcac caattgcacc
    56221 gagaattttc agctcttgcc cttcgtgtac ccggttacgt tcgataattt ccagttggtc
    56281 gatctgtaac tgttcagcaa gttccgctaa tgccatggtg atagcgaagt tacctttcgg
    56341 tgcgccataa ccctgataag caccatttgg gcaaatgttg ctgtagtagg tggtgacctg
    56401 gaaatcgacg ttatcgcacg gatataaggg cagcgacagc gccggtccgt tacacggtac
    56461 ggtgagtgag tggttgccgt aagggccagt gttggcgcgg aaatccatct tcactgccgt
    56521 caggcgacca tcttttttcg ctcccagttt gacggtgact ttcgcgacgt gacgagaggt
    56581 gttagcaata aactcttctt cacgggtgta gcggaacagt accggacgcc cggtcacgca
    56641 ggttgcccag gcgcacactt cttccagcag gatgtcctgt ttggaaccaa aaccgccgcc
    56701 aactcgctct ttaatgacat gaactttatg ctgtttcatg cccacgaggc gcgcgacctg
    56761 gcggcgtaag tgccatggta cctgggtgga ggcgtggata accagacgat cgccgtccat
    56821 acgagtaaag cagatatgtg tttcagtcgg gcactgctgc gcctgcgttg agttataggt
    56881 tcgctcaatg atcacatcgg catcggcaaa gcctttgtcc atatcgccaa tatgaccatg
    56941 aatactggcg gcgatatttt tgcgagggcg agaaccgatc gggaagttga tgatcatatg
    57001 ctcgccgcgc tgggctgcat ggctgttatc gtcttccaga gtatctggcg caccagcaac
    57061 atacaccacc ggttcatcgt gcacgacagg cgcatcttcc gccattgctt cgtcgatcga
    57121 cattaccggc ttaagcactt catattcaac gtcgatgagc ttcagtgctt cgagcgcaat
    57181 ttcttcactt tcagcgacga ccgcagcaac acgatcgccg acgtgacgca ttttcttgcc
    57241 gaacatacgg cggtcaagcg gtgacggttc cggtgcgctc tgaccccccg gggtatagta
    57301 gatatcaggg caattcaggt gagtaataac gtgaacgacg cccggtaagg cttcagcttt
    57361 gctgacatcc agatgagtaa tcagtgcgtg agcgtgtggg ctacgtaaca ttttaatgac
    57421 gcaggcgtca gccgttacgc ggtcttcaac atagcagggt ttcgcctgca ccattttcgc
    57481 ggcatcagtt ttaggataat gcttgccaat gacttcaagg tcgtcacgga aagtcggagc
    57541 gatatcgatg gtggcctgcg gattattttt acgtgcaacc gccagttcaa tgacctgata
    57601 gtattgctgc cagccagcat cgcggctgaa caaaccagaa agcgcgtcgt cgatctcttc
    57661 gcgggtaggt gcggcgatgc gatcgagaag atcggtgata atcagagctg cagctggatc
    57721 gttataacca gactgcacca cgccaacatc aaccattgcc tgttgaacca gacttaactc
    57781 gttccatttg cctagagatt ctgcggtacg aatatctgcc ttctctaact gtgcggcaat
    57841 aagcaaggac gcgttaacga tattaccgtt aaagattatt gcgtcagaac cggcaaaccc
    57901 gaaaccatca tcactgttgc gtacagagtg cattcccatg ttaaacaaca gcttttgcac
    57961 gttttcgcct ggattaacgg ttagctcctg aggcgcgcca tttaaagtaa agtggatgat
    58021 catacggctt cctccccagc ctgttggcag tcggcataca ggtcggctac cagaactccc
    58081 gtgatatagc gtttataggc cacgctgccc cgcaaatctt cctgcgggaa aatggcgttg
    58141 gcgacagcct gttcaagtga attgccttcc agattttgtt tttcgacatc gtgcagacgc
    58201 agtgctttac tggcgacgcc atccagcgca attcgcatac cgtcgtggtc tgtcgttgca
    58261 acggctgccg tcacgacggt taaacctgcc tgagaacggc taattttccg ggtcgcacag
    58321 gtgcgatacg gatctttaat gataatttcg gttagcaggc gatcgcatgg gcaggccagg
    58381 tagtcctcga ttgacagcgt ttcgccgttg ccaaaaacca gttcagcatc cagtgccagc
    58441 aggacgggaa gcagcactga ctcttcctgc cgggcggcga tttcaccacc aatggtcgac
    58501 tgattacgaa catggcgtga gtaaacaaaa ccgagagctt cacgcagtgc tgcaggaata
    58561 aatcgcgcat cacgcagtgg ctgcaagcga gacattgcac caatccgcag tgcaccgtta
    58621 tcccagtcaa cccagtccag ttccagatcc tgtaaggaaa tggcaatctt tttatcggta
    58681 cgggttggtg tagcattgag tttgctgcct ccggcgaacc agacggcttc atcctggtag
    58741 cggcgcttca gttccagcgc ctgttcgaca gagtcgggcc tgaaaaattg ttcaatcatc
    58801 ttctttcctt atttaaccct cttccctgcg ggaagagggc atctgtcatt tatgccagcg
    58861 catccatccg acgccacata ctggcagcgg cttttctggc ttgtgcataa atggaatcgc
    58921 aatcaaagtt aaactgacgg tcttcataga ccatcacgcc attgaccatc acgctgtgaa
    58981 cgctgcctga gcccataccg aaagcgatat gcccggcgat attgtctgcc agcagcggcg
    59041 tcggcgagtt gtaatcgcaa atggttaaat cggctttgta accggcttcc agaagaccaa
    59101 atttcgcgcc aaagttgcgg ctcatcagtt cgttaccgtt agtcagggct ttggcaaaac
    59161 tgtcaggcca cagcggacca cccgcatcgc gatgtttaaa gaaggcaaat ttcatctctt
    59221 caaacatgtc cgaaccaatg ccgtccgttc ccaacgccag attgcggatg tcgctaaggt
    59281 gatggttgta gccgacatgg ttgttcatgt ttgaacgggc gttatgcact aggaacgcat
    59341 cgcgctgatt gagtagggtg atgtcatctt tcgacaagta cagcccatga gcgaccagcg
    59401 ttttgctgtc gatgagatcg aattgcgcca gtcgtgccag caggtctttg ccgtaccagt
    59461 ggtgactgta ggaaacgtcg taaaggtctt ccgcagcgtg aatatgcaaa ccacggcctg
    59521 tggctttcac ggcttcacgc agcatctcca gaccggcatc tggcacggta aacggcgcgt
    59581 gagcaccgat atgtgcttcc accagatacg gctcggttgc tgctttcttc gcctcatcaa
    59641 tctgacgggc aaagcggatg ttttcttcta caccttcctg caactctttg atgccgttgt
    59701 tacggtcagt agtttcaaaa caggtcatcg cgcgcaggcc aacttttaaa aatgcgtcgc
    59761 gcaatgtgga gagcgacccg ccgatatacg ccggagaggc atggtgatcg ataaccgatg
    59821 tacatccgct cttaatcgct tccagggaac aaatcagtcc gctgtaatag agcgactctt
    59881 catcaagggc gcgatcgagc cgccaccaga gatttttcag cgttgagatg aaatccgggc
    59941 agggggcgat gtttgccata attccgcggg aaagccccga gtaaaaatgg ttgtgcgagc
    60001 agacaattcc cggcatcaca atccgaccat gcatctcttt gtaactggca tcggggtagc
    60061 gttgcgtcag ggcatcgccg atagcgacaa tcacatcatt ttcgatagcg atatcaacgc
    60121 cttcctgcac tttcgccggg tgtaactgca ctgcggtgac attcttcaga atcaacatga
    60181 ttacacctcc acgcggccca gcagataatg atgatgctgg tggacatggc tgatgatgcg
    60241 gcacatatcg ttcagctccg gtggtacgtt gttgaactga ccttcgctgt cgatgtttaa
    60301 cacccagctt tggttattca gtcgtactcg tacccggcaa tcttccacaa ggaagcctgg
    60361 gttgctgctg ttatcaaagt cttgcgccag gctgaaaacg gtgattttgt ctttgtatgg
    60421 tttaccgttc cacggacaga actgagcgca gttgccgcat tcgttacagt aagcgtcgag
    60481 gtgcagcgtc tggaatctat tctggaagcc tgggaccgca atggagacgt tggcgcggtt
    60541 cgggcagaca tccacacact tgctgcaaac gtagttacat tcgaggcagc gagcggcttc
    60601 ctgggcgaca aacgcgtcac gatcgtcact gttcaccagc gtgatagaga tatcgccttt
    60661 acgttgatag atttccgccg gattgacgtt gttccagtat ttatcgttct ggtgggaacg
    60721 gatattttcc cgactaagga tggcatcggt cgcccgacgc gcggttccga cagcagcgac
    60781 aatggaggat ggtccgcgct gtacgtcgcc gatcataaag acgtcagtca gacgagtttc
    60841 gccattatgg tcgacgtctg gccagccgtt tttgtccagc ggcacgccca tcgcattcag
    60901 ggcttcagta tcctgctgtt caccaatggc ggtgatcagg ctgtctacat gcagtgttac
    60961 tgtttcgttg gtttcaaccg gacgacgacg acctttctca tccggttcgc caagcgacat
    61021 aacgcgcaag gttaaggtgc catcagcatc gaaacgttcc ggattgttca ggaaacggaa
    61081 ctccacgccg tcgtgcaacg cttcttcata ctcttcgcgc catgcgggca tctcttgcag
    61141 tgaacgacgg taaacgatcg ttgctttttc tacgcctgga acgcgtaacg ccgcacgagc
    61201 gcagtccatt gcggtgttac ccgccccgac aacgaccaca tgtttgccca gcttgagcgc
    61261 tgtacccttg ttgtattcac gcaggaaggg gagtgatttc cagacatttt ggttgtcgcc
    61321 cgccagtttc acaccgctat ttttatcagt gccggtggca atcagaacat agtggaagcc
    61381 ctgatttttt aactgttcaa cggtcaaatc gggtgagcag ccatactcaa atttcacgcc
    61441 gtgagcggca acaaaatcga tatcgtgctg aattaactct gcaggaatac ggaactgagg
    61501 gatgatattt ttcaccacgc cgcccgcatt ggcttcgcgt tcaaacagcg taaccggatg
    61561 gcccgctctg gcaaggaagt aacctgcagc cagacccgcc ggacctgcac caatcacggc
    61621 aaccggatgg cgtgaaccag aaccggctgg tttgtgccag cgttgcttat attcatccca
    61681 acctttttcc agcgcgactt ttttcagttc gcggatattc agcgcactgt cgtaatccag
    61741 gcgggtacag ttgtattggc actggtgatc gcaaatatga ccggtaatgg cgggcagagc
    61801 gttgcgttgg tagatgagtt ccagcgcgtc ggcatagcgg tgttcgccaa gcagacggat
    61861 gtattccgga atatcttgct tgatagcgca ggcagtaaca cagggggcaa cgtagcagtc
    61921 ggtcagcggc aggtcttctg ccacttcaat acgctcttct ggcttccagt gtttctgggt
    61981 gtattccatg gttaacgcat ctgctgccag tctgttcagt cgttcgacgt caacatggtc
    62041 aagtccccag gcgtcggagc cttccagctc gcgcatgcag gcacttaagc gcagatagcc
    62101 gccaggtttc agcaggtcgg ttgccatagt aatagggcga atacctgtat caaaaatatc
    62161 gcggatagtc agctgactgg caccaccgga ataagaaatg ggcagtttgc cgtcaaaggc
    62221 gcgagagaga actgctgcaa cattgatgga gagcgggaac agcgcacggc ctgacatata
    62281 catctcttca ccaggcagtg cgcctttatt gttgatggtg ccgagagtgt tagtcagttt
    62341 tacgccaaag ccgagtgatt tttcttttgc cagtgccatc aggcgttcca gcatttccag
    62401 cgcttgcttc agcttgaggt cgtgatcaaa tgactcttct tttaagccta tgtaaccgaa
    62461 accgcagata tcgaggatct cacgaacacg cgcgtatccc agtaaggtcg ggttaagttt
    62521 cacaaaggtg ttgagccctt tttcttccag catgtagcgg caaatggctt cgatttcatg
    62581 cggaggacag ccgtgcatgg tggagagggt gacgccatgc accatactgg tggggatgcg
    62641 agcgggtaag gcttgcaagc tttcgcgttt ttcctgcaat ccgtggcgag ctaaaaatgc
    62701 gtcatcctgg agtaatttat tcagcgtatc gcgatattgc gcgaatttcg gatggtcaga
    62761 tgcgtccatc atattgtcga tgaactgttg cattggcggt tgcttaatac cttcgaggtt
    62821 gtaaccgacg ctcatattaa agatgaacga tttaccggaa tcagaaggtt ggaacatcgc
    62881 ttcgagaagg tgcagggcaa accaggcttt gaggtattca tcccaggctt taagcagggt
    62941 aaattcggta gaccattcgg tgttaaagca ctcgtcttcg gcatcaatac agggcttttc
    63001 cagctccagg cggtcaagaa tttggacggt ttttagttcg atgaaacgtc cgccagtcag
    63061 ccaggaagtg acaatatttt gcgcgagctg cgtgtgcggg ccagcggcag ggccgacggg
    63121 agtggcacag gtttcaccga agacgctaac agttttacct tttacaggtg agtaaaactg
    63181 ttgctcggga ataccaaaga ttgagcgttg ttgttggtat tcatcaaata tgcgcgtcaa
    63241 aagttcctca aacggaatgg gacgcataat atcccccata accctctcct tagcttatac
    63301 agatatcatt gagtgaccgg tgttgagaaa tccggtggtt tagggggata gagcaacaat
    63361 cgcaccactc ttgccaggtt tggcgggaaa tataaaaaaa tgtgaggcgg ctcgaaactt
    63421 aagggctttt tgtgaatgaa ctcacaggtg ccaaatgaga attaaaatca ctcctgaaaa
    63481 ttattattgc cagcccattt atcacttttt ctcttttctc aaaatcagtg attccttatc
    63541 attatgataa agagtgctgg agggattaaa tatttgaacg caatcgttta actacatttt
    63601 ctaaatttta gtataccttt tttgcgctgt aataaagtcg tccggagtat caatatctaa
    63661 aattatttct gcattttcta tttcgacgga ataatgatct cccataagga gtgcctgacg
    63721 catattggtg acattgggtc gctggattgc ctgcatcagg catggttttg acactaaaat
    63781 cggatggccg gggatgccat tatggagtgg cagtattgcg ccatcatttc gtaccgacca
    63841 gatttttcta aaaatatcaa tggtgagggt tggcatgtca ccatgggtga gaaaacaatg
    63901 ttctgtttgc actgcgggta ctgcggcctt gacggatgtt agtaaaccct gcgcataatc
    63961 tgggttgtga ataatagtga tattgctctg gttcgcataa cgttcgtgca gttcattacc
    64021 acgatagccg gtgactaaaa taattcggct acaaaactgc aacgcatttt tgatacttgt
    64081 atcaagaatt gttccctgtt gccagggtaa catcattttc cattgcccca ttcttgatga
    64141 taatccggca gcggtaatta tacagtcgat ggctgacata gtggttccat tatttatagg
    64201 ggctaataac gcctgataat tgcgttactt cctgatggat taagttgggg ataatttatc
    64261 ccagagaggt cataaagact cgtgaaaagt atagttgact catcggtatt attcattgat
    64321 ttaggtgcgc agaaacgtcc cactgtgatt tctgttgttg gtgctggggg gaaaaccagc
    64381 ttgctttttt ggctggcaga actgctccag gctagcggca ggcgtgtgtt aatcacgacg
    64441 acgacacata tgtttatgcc aacgtctcac tggcccgtgg ttttctgtcg tgatcccgcc
    64501 atgcttcctc atgcgtctct tacatccccc atttcatttt gttttcacag ttggaaagcg
    64561 aaccagggaa aagtgcaggg atttacgcca gaagcgattg atgcactggt gcaacgacca
    64621 gagtgtgacg taattctcat tgaagcagat ggctcgcgtg gaatgccgtt aaaagcgcct
    64681 gatgagcacg aaccttgcat acccaaaagc agttgctgcg tgattgctgt gatgggagga
    64741 catattttgg gcgcgaaagt gagcacagaa aatgtccatc gctggtcgca gtttgctgag
    64801 attaccgggt taacacctga tgcaaccttg caactgagcg atctcgttgc gctggttcgc
    64861 caccctctgg gggcgtttaa aaacgtaccg caaggttgtc ggcgagtctg gttcattaac
    64921 cgtttttctc aatgtgagaa tgcgattgcg caaagcgagt tccttcaacc gctgcaacaa
    64981 cacgacgtag aggcaatctg gctgggcgat atacaagaac atcctgcaat cgcgcgcaga
    65041 tttgtgaatt agcggcccgg gtattccggg ttactgaaag gatatccatt gaggttggta
    65101 tgaatatttt cacagaggct gcaaaactcg aagagcaaaa ttgtccgttt gcgatggcgc
    65161 aaattgttga tagccgaggc tcgacccccc gccattctgc acaaatgtta gtgcgcgccg
    65221 atggttctat cgtcggtaca attggtggcg gaatggttga gcggaaggtg attgaagagt
    65281 cgcttcaggc attgcaggaa cgtaagccgc gattattcca tggacgtatg gctcgtaacg
    65341 gtgcggatgc tgtcgggtca gattgtggag gtgcaatgtc agtatttatc agcgtccatg
    65401 gtatgcgtcc acgtctggtg ttgattggcg cggggcatgt caaccgggcg atagcccaga
    65461 gtgcggcgct attaggattt gatattgccg ttgctgatat ttatcgcgaa agcctcaatc
    65521 ctgaactatt cccaccatca accacgcttc tccatgctga gtcgtttggt gcggcagtgg
    65581 aagcactgga tattcgccct gataattttg tcctgattgc cacgaataat caggatcgtg
    65641 aagccctcga caaactcatt gaacagccca ttgcatggtt ggggttgctg gcaagtcgtc
    65701 gcaaggttca gcttttcctg cgtcaattgc gtgagaaagg cgtggctgaa gaacatattg
    65761 cccgtttaca tgcgcccgtt ggttacaaca taggtgcgga aacgccgcag gagatcgcca
    65821 tcagcgtgct ggcagaaata ttacaggtga aaaataacgc gccgggtggg ctgatgatga
    65881 aaccttctca tccttccgga caccagctgg tggtgattcg cggtgcggga gatatcgcca
    65941 gtggtgtggc gctacgtctg tatcatgcgg gttttaaagt gatcatgctg gaagtggaaa
    66001 aaccgacagt gattcgttgt accgtggcgt ttgcccaggc cgtgttcgat ggcgaaatga
    66061 cggtcgaagg cgtcactgct cgcctggcaa ccagctctgc ggaagcgatg aaacttaccg
    66121 aacgcggatt catccctgtg atggtagatc ccgcctgttc attgcttgat gaactgaaac
    66181 cgctttgcgt ggtggacgct attctggcga aacagaattt gggaacgcgg gcagatatgg
    66241 caccagtaac aatcgcgctt gggccgggct ttactgctgg gaaggattgt catgcggtaa
    66301 ttgaaacaaa tcgcgggcac tggctcggtc aggtgattta ctctggttgt gcgcaggaga
    66361 ataccggtgt tcctggcaat attatggggc ataccacccg acgggtgatc cgtgctcctg
    66421 ctgcaggcat tatgcgatcc aacgtgaaat taggcgatct ggtgaaagag ggcgatgtga
    66481 ttgcctggat tggtgagcat gaaattaaag caccgttgac ggggatggtg cgtggcttgt
    66541 tgaacgacgg cctggcagtg gttggtggtt ttaaaattgg tgatatcgat cctcgtggtg
    66601 aaaccgctga tttcaccagc gtttctgata aagcccgggc gattggcggc ggcgtacttg
    66661 aggcgttaat gatgttgatg catcagggcg tgaaagcgac aaaagaagtg ctggaagtgg
    66721 cttaaatgaa aatggccgga tggtattacc cacccggcct taaaattact gacaaatcac
    66781 cgttccggtt ttgccttcaa tcccttcttt cgctttgctc aatacggtaa tcagtgcttc
    66841 acgacctgcg cgagagcgag caaacgaggc ggcagcttcc actttaggca gcatagaacc
    66901 tttcgcaaaa tgaccttctt cgataaagcg ttcagcatca cttaatgaca ggcgatcgag
    66961 ccactgttca ttctctttac cgaagttaat ggccactttt tctaccgcag tcagaatgat
    67021 cagcatatcg gcatcaatca tttctgctaa acgggcgctg gcccagtctt tatcgataac
    67081 cgcgctggca ccacgcagat ggttaccttc acgaataaca gggataccac caccgccaac
    67141 ggtgatcacc acctggcctg catctaccag agctttaacc gtttcttttt caatgatatc
    67201 aaccggtttt ggcgaggcaa caacacggcg atagccgcga ccagcatctt ctttcagagt
    67261 gtaaccctgt tttgtcagtt gctctgcttc ttgcttggta aagaacgaac cgatcggctt
    67321 ggtcgggttg aggaatgccg gatcgttagc gtcgacttcg acctgagtaa ccagcgttgc
    67381 tacaggttta ttaatgcccc gagaaagcag ttcttctctt aacgcgtttt gtagatcgta
    67441 accaatataa ccctggctta aggcaacaca gacagacatc ggcagcattg gcgagtgtgc
    67501 ttcagttttt gcggcagctt caaaggcctg gttaatcatg cccacctgtg gcccgttacc
    67561 gtgagtgacg atgacctcat gtccctgggc aattaaatca acaattgcct gagaagtaat
    67621 ttttaccgct ttcatttgcc cggccagatc atcgcccagc gcattcccgc ccagggcgag
    67681 aacaattttc ttactcattt ttcctcactc atcattcttg attgtgtata gatccctggc
    67741 agtggttaga gcacgggagg gacaaacggt ttgcgtcgca ggaaacgacc tcgcccggct
    67801 ttgcctgtaa aggtgccgtc acagaaaatc gtttcaccac gggataaggt tctgacaatc
    67861 gcgccctgac aggtaaaacc ctcccatggc gagtagtcgg cgttgtcgtg gagatggcga
    67921 tgctgaattt gttggctctg acgtgggtcg ataatcacca cgtcgccatc ggaaccgggc
    67981 gctaataatc ctttttgcgg ccacaggcca aacagcctgg cgggcattgc gctggttaat
    68041 tcaacaaagc gttccggtgt tatacgtccc gtcatcacgc cactggaaaa cagtaactgc
    68101 atgcggtttt ccacgccggg taagccattt gggcagcgac tgaaatcgcc tttagaaatt
    68161 tgcaggcgtt gagccattga gaacgtgcag tgatcggtcg ccaccacgtc aatcgcacca
    68221 tcgctgatgc cacaccacag tttgtcctgc tcgcgtacgt tacgcagcgg tgggctaaga
    68281 atgaacttca tgccatcttc tgtatcgtaa ctgcgttcgt ccaacaggag atattgtggg
    68341 caggtttcaa cccagactgg ctggtgattc gcacgggcaa gacgcagata atccagacct
    68401 aagccgttag acaggtgcac gatatagagc ggggcgttac cggcaatttg tgccaggtta
    68461 atcatgcggg cgatggcttc cgcttcgcat tccagagggc gactcaaggc gtgatagcgc
    68521 ggcgcggtta accctgcggc gataaactcc gcccgcttgc tggcgatagc cgcatcattt
    68581 tccgggtgca cggtggtcag cgcgccggat tcatgcagac ggcgtaatgc ctgcaaaacc
    68641 tcgtcatcgt tgagtttgta ttgataggtt aaatagagtt taaaactgct cagcccttcc
    68701 tcgaccatca tagggatttc gtcgaggatg gcgtgattaa tgtgctggat cacaccatga
    68761 aagctgtagt cgatgaccgc cttatgggcg gcatagccac gataaacctc cagttgatgg
    68821 cgtaaccgac acccgtttgg gccaaatccc atatggtcaa taatggttgt tgtaccgcca
    68881 cacgcagctg cgcgggtacc ggtaaaaaaa tcatcacaac tgcgcgcgat gccgacatca
    68941 atattgaaat gcgtatggac atccacgccg ccagggaaaa cgtaacagcc agtggcatca
    69001 atttcttcat acgggagctg cggcgaaata ttgttgccca actggcgaac aatcccgctt
    69061 tcaatcagca aatcctgttt ggcttgtcca tctgcgttaa cgacagtgcc gtttttgatc
    69121 aatacgcgca tagcaaactc caaaaaccct ccggcgaacc ggaggggaat aagtgaagag
    69181 taattattcg gttgccagcc agcttaacgg gattgcagcg tacatcgcag cacaggtcac
    69241 caggtgagat ttccaggttt tttcgttagg tgcatgcgct tcaggttctt tacccgggcc
    69301 aaagccgatc accggaatgc cgtgacggcc catgatagat acgccgttag ttgagaaggt
    69361 ccacttatca acaaccggcg ctttgccaaa cagcccttcg taggcattca ccagtgcttt
    69421 aacggtgaag tgatcttctt ccactttcca ggtcgggaag tagcattcgg ttgggtaaac
    69481 caggccagtc caggacggac ggtcgtagtt gtacatagaa acaaccgcgt tagctttctg
    69541 tactgcaggc agggcgcgga tttcgtccag cgcgccttcc caggtttcgc cccaggtcag
    69601 acgacggtcg attgaaactg cgcagctgtc tgctacagcg caacggcttg gggatgtgaa
    69661 gaagatttca gaaacggtga gggtgccttt gccgaggaat tcgtcataac ccagacgttg
    69721 ggagagttct tgtaattcgc caagaatcgg acccattttg aaaatggcgt tatcaccgcg
    69781 ttctggtgca gaaccgtggc agctaacacc ctgaacatca atacgaattt ccatacgacc
    69841 gcgctgacca cggtatatct ggcagtcggt tggttcggta ctgaccacaa attccgggcg
    69901 aatgccggat tgttcaataa tgtactgcca gcacagaccg tcgcagtctt cttcctgcac
    69961 agtaccggta accagcaggg tatattcatc ttccagaccg aggtctttaa tgattttacc
    70021 ggcataaacc atagatgcca tgccgccttc ctggtcggaa gtaccgcgac caccgattag
    70081 ctcatcagtt tccatgcctt cgtacggatc gaagtcccag tttttgatgt tgccaatgcc
    70141 gacggtatcg atatgagcgt ccattgccac cagacgcggg ccgtggccga tataaccgag
    70201 aacgttgccc atcgggtcga tttcaacttt atcgaaaccg actttttcca tctcttcttt
    70261 aatacgatgt actacgcgtt tttcgtcgca gctttcactg ggaatagcaa ccatgtcgcg
    70321 caggaagcga gtcatgtccg cctggtaatc ttttgctttt tcaagaatca gtttgaatgg
    70381 aatattctta gccattattt ttctccagat tttttactcc ccgttttgca gggagtagtt
    70441 ccaatagggt gattaaggtg ctacagcgtg tttgccttcc cagacaactt cgcggtagtg
    70501 cttcacgtcg gtgtcgcctt cagtactgat aaccagcact acggcatctt tgttcagcgc
    70561 cagtttttcc atcaggcttt gacgttgcgg gtgataatga accgctgcga gaacgcccaa
    70621 accgacagcg ccggattcac cggagatgat gcgcgggtcg ttgccgtacg gattacccag
    70681 cacgcgcata cctaatgcgg caacgctgtc ctggcaggag atgaattggg tggcgcagtt
    70741 acgtaggatt tcccagccca gcgggttagg ttcgccacag gccaggcccg ccatgatggt
    70801 ggccatatca ccgccaacgt tgacgatgtc gcctttgacg ccggagcgat aaatacagtc
    70861 agctttgtca ggttcaacaa taatgctgtg cagattttgc gggctgtaga cgtcgaccag
    70921 ataacccagc acaccaccgg ccattgctcc gacaccggct tgcagcagaa cgtgcgtcgg
    70981 ggttacgccc atttcacgca tttgctcgac ggcttcatct gccagggttg cgtagccttg
    71041 catgatccag gttgggattt tggtgtaacc ttcccatgcc gtgtcctgta ccacttccca
    71101 gccgtgctgc tgcgcgtgtt gcatggtcag gcgaacggta tcgtcatagt tcatatctgt
    71161 gacgatgcac tcggcaccga ggttcagaat ggcgtcaacg cgttcctgag cagaaccttt
    71221 cggcatgtaa atcaccgcat tctgtccgag ttgctgtgct gcccacgcca caccgcgccc
    71281 gtggttgccg tcggtggtgg tcgcgaaagt cattttttcg ccgatggcat ttttcaggtg
    71341 ctcaaatgac agcgtttcga tatcaagatg atatttttca cacaataatt gagcgatggc
    71401 gtacgcaccg ccaagcattt tgaaggcgtt cagaccgaat cgttttgatt cgtctttgac
    71461 gagaattttc ttcacaccaa aaaggtttgc gagatcgtcc agcgcacaaa gcggtgttgg
    71521 gcgataacca gctattttct ggtggaactg gcgcgccagt ttggcctggc tttgcgaaaa
    71581 gagcggtgat gtttcgccgt tgaaaaattt gttatcggcg atatcaatct tcaatgagaa
    71641 aacggacata tcctatcctt ttggcctctg gaaatagagg gccgaccagc tgatcggccc
    71701 gaggggttat ttcacgcgtt cttgcgcttc tttcagcagt tgctcgagca gtgcacctgg
    71761 tttgacgtat ttacgggaca ggatcatcgc ggcgatgata taaggcttcc agctggcttc
    71821 tttgtaggta gcgatacggt atttttcgaa tacgccttca gtcacttcac cttctttaca
    71881 ggatacgccg ctgatatcag ctggcaggca gtgcatgtac agggcttcgc catcacgggt
    71941 cagttccatc atctcttcag tacaatgcca gtctttgtgt tgcgcgttct gtgccagaca
    72001 ctgtttttcc agtgctttta agccttcgtg atcgttcgca cgcagcaatt cagtacgctc
    72061 ttccatcact ttgtaaggtg cccatgactt cggataaacg atgtctgcgt ctttgaaggc
    72121 ttcttccatg ctggtgacct gacggaagct accaccggag gctttagcat tgtttttcgc
    72181 cacttcaacc acatccggga tcaggtcgta gccttccgga tgggccaggg tgacatccat
    72241 accgaagcga gtcatcagac cgatgatgcc ttgtggtaca gagagcggtt tgccatagct
    72301 tggagagtag gcccaggtca tggcgatttt tttacctttc aggttttcca gtgaaccaaa
    72361 gtgttcacgt aaccacgcga ggtcagccat tgactgagtc gggtggtcaa tatcgcattg
    72421 caggttcact aaagccggac gctgtggcag tacaccctgc ttgtaaccgt catcaagtgc
    72481 agcgccaact tcacgcatat aggcgttgcc tgcgcccaga tacatatcgt cgcgaatacc
    72541 aatagcgtcg gcgcagaagg agatcatatt ggcggtttca cgcacggttt cgccgtgagc
    72601 gatttgtgat ttgccttcgt cgagatcttg ttgtgcaagg ccgagcaggt taagcgcgga
    72661 agcataagag aagcgggtac gggtggagtt gtcgcggaat acggaaatac ctaatccact
    72721 attaaagact ttggttgaga tgttttcagc acgcagtgct tttaatgctg cggcaacgtc
    72781 cagtacttgt ttcagttcat ctggcgtctg ttcccacgtt aacaaaaaat ctttctcgtg
    72841 aaggtgagag gtcagcgaat tgatatcctt aatcagctca ttaacagttt tcatcataaa
    72901 gtcccggtag tggaatgaca ggcaaagtgg tttatttcac tgtgacttgc caaagtgatt
    72961 ggtgttttca cagctatacc tgggactaac aataagtgtg ccagaattat ccgcgcggct
    73021 gcaaaagact aatttgacgg ataatgtgtg gggttgatca caaattgatg ccagcttctg
    73081 gttgtttgta aaatacgaaa acgtttggct ttttttatcg cgttatttat ttgctgctaa
    73141 gtatcagaaa taagataaag gggcaggcta tctct
//
LOCUS       DAOFQD010000007        70372 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.007, whole genome shotgun sequence.
ACCESSION   DAOFQD010000007 DAOFQD010000000
VERSION     DAOFQD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.007"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            145..1329
                     /locus_tag="QBF46_000460"
     CDS             145..1329
                     /locus_tag="QBF46_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDQ6672693.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1440..2363
                     /locus_tag="QBF46_000461"
     CDS             1440..2363
                     /locus_tag="QBF46_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ6672694.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAELLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(2367..3185)
                     /gene="nlpA"
                     /locus_tag="QBF46_000462"
     CDS             complement(2367..3185)
                     /gene="nlpA"
                     /locus_tag="QBF46_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDQ6672695.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            3407..3700
                     /locus_tag="QBF46_000463"
     CDS             3407..3700
                     /locus_tag="QBF46_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672696.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(3741..4931)
                     /gene="nepI"
                     /locus_tag="QBF46_000464"
     CDS             complement(3741..4931)
                     /gene="nepI"
                     /locus_tag="QBF46_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDQ6672697.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(5142..5594)
                     /locus_tag="QBF46_000465"
     CDS             complement(5142..5594)
                     /locus_tag="QBF46_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDQ6672698.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5647..6981)
                     /gene="adeQ"
                     /locus_tag="QBF46_000466"
                     /gene_synonym="yicO"
     CDS             complement(5647..6981)
                     /gene="adeQ"
                     /locus_tag="QBF46_000466"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDQ6672699.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            7156..8922
                     /gene="adeD"
                     /locus_tag="QBF46_000467"
     CDS             7156..8922
                     /gene="adeD"
                     /locus_tag="QBF46_000467"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDQ6672700.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGKTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8967..10358)
                     /gene="uhpT"
                     /locus_tag="QBF46_000468"
     CDS             complement(8967..10358)
                     /gene="uhpT"
                     /locus_tag="QBF46_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDQ6672701.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10496..11815)
                     /gene="uhpC"
                     /locus_tag="QBF46_000469"
     CDS             complement(10496..11815)
                     /gene="uhpC"
                     /locus_tag="QBF46_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDQ6672702.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(11825..13327)
                     /gene="uhpB"
                     /locus_tag="QBF46_000470"
     CDS             complement(11825..13327)
                     /gene="uhpB"
                     /locus_tag="QBF46_000470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDQ6672703.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEKAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(13327..13917)
                     /gene="uhpA"
                     /locus_tag="QBF46_000471"
     CDS             complement(13327..13917)
                     /gene="uhpA"
                     /locus_tag="QBF46_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDQ6672704.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13992..14282)
                     /gene="ilvN"
                     /locus_tag="QBF46_000472"
     CDS             complement(13992..14282)
                     /gene="ilvN"
                     /locus_tag="QBF46_000472"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDQ6672705.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(14286..15974)
                     /gene="ilvB"
                     /locus_tag="QBF46_000473"
     CDS             complement(14286..15974)
                     /gene="ilvB"
                     /locus_tag="QBF46_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDQ6672706.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVVDLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(16080..16178)
                     /gene="ivbL"
                     /locus_tag="QBF46_000474"
     CDS             complement(16080..16178)
                     /gene="ivbL"
                     /locus_tag="QBF46_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDQ6672707.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            16743..16832
                     /gene="tisB"
                     /locus_tag="QBF46_000475"
     CDS             16743..16832
                     /gene="tisB"
                     /locus_tag="QBF46_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDQ6672708.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            17112..18296
                     /gene="emrD"
                     /locus_tag="QBF46_000476"
     CDS             17112..18296
                     /gene="emrD"
                     /locus_tag="QBF46_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDQ6672709.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(18304..18801)
                     /locus_tag="QBF46_000477"
     CDS             complement(18304..18801)
                     /locus_tag="QBF46_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDQ6672710.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(18798..19160)
                     /locus_tag="QBF46_000478"
     CDS             complement(18798..19160)
                     /locus_tag="QBF46_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDQ6672711.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(19150..19497)
                     /locus_tag="QBF46_000479"
     CDS             complement(19150..19497)
                     /locus_tag="QBF46_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDQ6672712.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            19606..20055
                     /locus_tag="QBF46_000480"
     CDS             19606..20055
                     /locus_tag="QBF46_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672713.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(20102..21595)
                     /locus_tag="QBF46_000481"
     CDS             complement(20102..21595)
                     /locus_tag="QBF46_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDQ6672714.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARSGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDGTF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDGGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIILSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(21592..23307)
                     /locus_tag="QBF46_000482"
     CDS             complement(21592..23307)
                     /locus_tag="QBF46_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDQ6672715.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            23474..24367
                     /locus_tag="QBF46_000483"
     CDS             23474..24367
                     /locus_tag="QBF46_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDQ6672716.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTRIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(24364..25686)
                     /locus_tag="QBF46_000484"
     CDS             complement(24364..25686)
                     /locus_tag="QBF46_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDQ6672717.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALAPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEILCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(25686..27302)
                     /locus_tag="QBF46_000485"
     CDS             complement(25686..27302)
                     /locus_tag="QBF46_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDQ6672718.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRTMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            27598..28235
                     /locus_tag="QBF46_000486"
                     /pseudo
     CDS             27598..28235
                     /locus_tag="QBF46_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(28232..29893)
                     /locus_tag="QBF46_000487"
     CDS             complement(28232..29893)
                     /locus_tag="QBF46_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDQ6672719.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(30089..30517)
                     /gene="ibpB"
                     /locus_tag="QBF46_000488"
     CDS             complement(30089..30517)
                     /gene="ibpB"
                     /locus_tag="QBF46_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDQ6672720.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(30629..31042)
                     /gene="ibpA"
                     /locus_tag="QBF46_000489"
     CDS             complement(30629..31042)
                     /gene="ibpA"
                     /locus_tag="QBF46_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDQ6672721.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(31036..31108)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            31348..31680
                     /locus_tag="QBF46_000490"
     CDS             31348..31680
                     /locus_tag="QBF46_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDQ6672722.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(31682..32896)
                     /locus_tag="QBF46_000491"
     CDS             complement(31682..32896)
                     /locus_tag="QBF46_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDQ6672723.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSDKYVFIHGPENPDETWHYDFHHRRGVIAK
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPDGERVSFTYNDHVMHQLDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLTWM
                     EGGQLWITETDR"
     gene            32997..34061
                     /gene="cbrA"
                     /locus_tag="QBF46_000492"
     CDS             32997..34061
                     /gene="cbrA"
                     /locus_tag="QBF46_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDQ6672724.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(34058..35395)
                     /locus_tag="QBF46_000493"
                     /pseudo
     CDS             complement(34058..35395)
                     /locus_tag="QBF46_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(35470..36618)
                     /gene="dgoD"
                     /locus_tag="QBF46_000494"
     CDS             complement(35470..36618)
                     /gene="dgoD"
                     /locus_tag="QBF46_000494"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDQ6672725.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(36615..37232)
                     /gene="dgoA"
                     /locus_tag="QBF46_000495"
     CDS             complement(36615..37232)
                     /gene="dgoA"
                     /locus_tag="QBF46_000495"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDQ6672726.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(37216..38094)
                     /gene="dgoK"
                     /locus_tag="QBF46_000496"
     CDS             complement(37216..38094)
                     /gene="dgoK"
                     /locus_tag="QBF46_000496"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDQ6672727.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREEKTPVVMAGMVGSNVGWKVVPYLSVPACFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(38091..38780)
                     /gene="dgoR"
                     /locus_tag="QBF46_000497"
     CDS             complement(38091..38780)
                     /gene="dgoR"
                     /locus_tag="QBF46_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDQ6672728.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERSWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            39058..39714
                     /locus_tag="QBF46_000498"
     CDS             39058..39714
                     /locus_tag="QBF46_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672729.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(39760..40572)
                     /gene="yidA"
                     /locus_tag="QBF46_000499"
     CDS             complement(39760..40572)
                     /gene="yidA"
                     /locus_tag="QBF46_000499"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDQ6672730.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(40687..41085)
                     /locus_tag="QBF46_000500"
     CDS             complement(40687..41085)
                     /locus_tag="QBF46_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDQ6672731.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(41325..43739)
                     /gene="gyrB"
                     /locus_tag="QBF46_000501"
     CDS             complement(41325..43739)
                     /gene="gyrB"
                     /locus_tag="QBF46_000501"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDQ6672732.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(43768..44841)
                     /gene="recF"
                     /locus_tag="QBF46_000502"
     CDS             complement(43768..44841)
                     /gene="recF"
                     /locus_tag="QBF46_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDQ6672733.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(44841..45941)
                     /gene="dnaN"
                     /locus_tag="QBF46_000503"
     CDS             complement(44841..45941)
                     /gene="dnaN"
                     /locus_tag="QBF46_000503"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDQ6672734.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(45946..47349)
                     /gene="dnaA"
                     /locus_tag="QBF46_000504"
     CDS             complement(45946..47349)
                     /gene="dnaA"
                     /locus_tag="QBF46_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDQ6672735.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            47646..47726
                     /locus_tag="QBF46_000505"
     CDS             47646..47726
                     /locus_tag="QBF46_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672736.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            47956..48096
                     /gene="rpmH"
                     /locus_tag="QBF46_000506"
     CDS             47956..48096
                     /gene="rpmH"
                     /locus_tag="QBF46_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDQ6672737.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            48113..48472
                     /gene="rnpA"
                     /locus_tag="QBF46_000507"
     CDS             48113..48472
                     /gene="rnpA"
                     /locus_tag="QBF46_000507"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDQ6672738.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            48436..48693
                     /gene="yidD"
                     /locus_tag="QBF46_000508"
     CDS             48436..48693
                     /gene="yidD"
                     /locus_tag="QBF46_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDQ6672739.1"
                     /translation="MAPPLSPGSRVLIALIRVYQLLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            48696..50342
                     /gene="yidC"
                     /locus_tag="QBF46_000509"
     CDS             48696..50342
                     /gene="yidC"
                     /locus_tag="QBF46_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDQ6672740.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            50448..51812
                     /gene="mnmE"
                     /locus_tag="QBF46_000510"
     CDS             50448..51812
                     /gene="mnmE"
                     /locus_tag="QBF46_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDQ6672741.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            52055..52129
                     /gene="tnaC"
                     /locus_tag="QBF46_000511"
     CDS             52055..52129
                     /gene="tnaC"
                     /locus_tag="QBF46_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDQ6672742.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            52350..53765
                     /gene="tnaA"
                     /locus_tag="QBF46_000512"
     CDS             52350..53765
                     /gene="tnaA"
                     /locus_tag="QBF46_000512"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDQ6672743.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            53856..55103
                     /gene="tnaB"
                     /locus_tag="QBF46_000513"
     CDS             53856..55103
                     /gene="tnaB"
                     /locus_tag="QBF46_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDQ6672744.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            55236..56411
                     /gene="mdtL"
                     /locus_tag="QBF46_000514"
     CDS             55236..56411
                     /gene="mdtL"
                     /locus_tag="QBF46_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDQ6672745.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            56386..57345
                     /gene="yidZ"
                     /locus_tag="QBF46_000515"
     CDS             56386..57345
                     /gene="yidZ"
                     /locus_tag="QBF46_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDQ6672746.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            57502..58251
                     /locus_tag="QBF46_000516"
     CDS             57502..58251
                     /locus_tag="QBF46_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDQ6672747.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            58273..58839
                     /gene="yieF"
                     /locus_tag="QBF46_000517"
                     /gene_synonym="chrR"
     CDS             58273..58839
                     /gene="yieF"
                     /locus_tag="QBF46_000517"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDQ6672748.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(58893..60230)
                     /gene="adeP"
                     /locus_tag="QBF46_000518"
     CDS             complement(58893..60230)
                     /gene="adeP"
                     /locus_tag="QBF46_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDQ6672749.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            60395..61060
                     /gene="yieH"
                     /locus_tag="QBF46_000519"
     CDS             60395..61060
                     /gene="yieH"
                     /locus_tag="QBF46_000519"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDQ6672750.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            61127..61600
                     /locus_tag="QBF46_000520"
     CDS             61127..61600
                     /locus_tag="QBF46_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HDQ6672751.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            61649..62236
                     /locus_tag="QBF46_000521"
     CDS             61649..62236
                     /locus_tag="QBF46_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDQ6672752.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(62298..63020)
                     /locus_tag="QBF46_000522"
     CDS             complement(62298..63020)
                     /locus_tag="QBF46_000522"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDQ6672753.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVYIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(63035..64204)
                     /locus_tag="QBF46_000523"
     CDS             complement(63035..64204)
                     /locus_tag="QBF46_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDQ6672754.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYHALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDITGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(64231..65847)
                     /gene="bglH"
                     /locus_tag="QBF46_000524"
     CDS             complement(64231..65847)
                     /gene="bglH"
                     /locus_tag="QBF46_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDQ6672755.1"
                     /translation="MFRRNLITSAVLLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(65933..67327)
                     /gene="bglB"
                     /locus_tag="QBF46_000525"
     CDS             complement(65933..67327)
                     /gene="bglB"
                     /locus_tag="QBF46_000525"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDQ6672756.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(67346..69223)
                     /gene="bglF"
                     /locus_tag="QBF46_000526"
     CDS             complement(67346..69223)
                     /gene="bglF"
                     /locus_tag="QBF46_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDQ6672757.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(69357..70193)
                     /gene="bglG"
                     /locus_tag="QBF46_000527"
     CDS             complement(69357..70193)
                     /gene="bglG"
                     /locus_tag="QBF46_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDQ6672758.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT        17418 a        18507 c        17453 g        16994 t
ORIGIN      
        1 aatctcaatt gctggcccta taatattgcg ctagcattgc tttctggttg tatcagcgat
       61 actcaaaaat tcttaataca atactcattc gactggtact tatttgtaac tcagttatat
      121 tttttcgccc ggtgattcag aagaatgcaa aaaacggcta ccactccatc aaaaatactt
      181 gatctcactg ccgcggcatt tttacttgtc gcctttctga cgggtattgc gggcgctctt
      241 cagactccta ccctaagtat attcctcgca gatgaactga aagcccgtcc tataatggta
      301 ggttttttct tcaccggtag cgctattatg ggaattctgg tcagtcaatt tctggcaagg
      361 cactccgata aacaaggcga ccgtaaatta ctgattctgc tatgttgctt atttggagtg
      421 ctggcctgca cgctttttgc gtggaatcgc aactacttca ttctcctctc aacgggcgta
      481 cttctgagta gttttgcttc caccgcaaac ccgcaaatgt tcgccctcgc ccgtgaacac
      541 gccgacagaa caggccgtga gacggtcatg ttcagtacat ttttacgtgc tcagatctcg
      601 cttgcctggg ttatcgggcc accgctcgct tatgaactgg caatgggatt tagttttaaa
      661 gtgatgtatc tcaccgctgc catcgcattt gttgtttgcg gactgatagt ctggttgttt
      721 ttgccatcaa tacaaagaaa tattcctgtc gttacccaac ccgtagaaat tttaccctcc
      781 acccacagga agcgggatac gcggctactt tttgtggtct gttcaatgat gtgggcggcg
      841 aataatctct acatgataaa tatgccgcta tttattattg atgaactgca tctaaccgat
      901 aaactggctg gagaaatgat tggtatcgct gccggtctgg aaattccgat gatgttaatc
      961 gcaggctatt acatgaaacg tattggcaag cgactattaa tgctcattgc tatcgtgagt
     1021 ggaatgtgtt tttacgccag cgtactcatg gcgacgactc cggcggttga gctggaattg
     1081 caaattctta atgccatctt ccttggtatt ctctgtggta tcggcatgct ttattttcag
     1141 gacttgatgc ctgaaaaaat aggctctgcg acaacgttat atgcaaatac ttcacgcgtc
     1201 ggctggatta tcgccggctc tgttgacgga attatggttg aaatctggag ctaccacgcg
     1261 ttgttctggc tggcgatagg gatgttgggt attgcgatga tttgcctgct gtttattaaa
     1321 gatatttagc tcaccagtct gggctaagaa aaatcggcgt ggcgcaaaca gagccatcct
     1381 cgccattttt gctatcatgc ctgcatacat aaacgacaaa acagtatgca gagggaaaaa
     1441 tgggttccac cagaaagggg atgctgaacg ttctgattgc cgccgtgttg tggggaagtt
     1501 caggggtctg cgcgcaatac atcatggagc aaagccagat gtcgtcgcag tttttgacta
     1561 tgacgcgttt gatattcgcc ggtttgattc tgctgacgct gtcatttgtt catggcgata
     1621 aaatcttttc tattattaac aatcacaaag atgccattag tctgctgatt ttttccgtgg
     1681 ttggcgcgct caccgtacag ctcacttttt tgctaaccat cgaaaaatcg aacgcagcca
     1741 cggcaacggt gctgcaattc ctctcaccga cgattatcgt cgcctggttc tcactggtgc
     1801 gtaaatcgcg cccgggcatt ctggttttct gcgctatttt gacatcgctg gtcgggactt
     1861 ttttattggt gacacacggt aatccgacgt cattatcgat ctctcctgcc gcgttgttct
     1921 ggggcattgc ctcggcattt gctgctgcat tctataccac ctatccctca acgctaattg
     1981 cccgctatgg cacgttacca gtcgtcggct ggagtatgct gattggcggt ctgattctgt
     2041 tgccttttta tgccagacaa ggaacaaact ttgtcgttaa cggcagtttg attctggcgt
     2101 ttttttattt ggtggtcatt ggtacgtccc tgacatttag tctgtacctg aaaggagcac
     2161 aattaattgg cggtccaaaa gccagcattt tgagctgtgc agaactatta agtagcgcgc
     2221 tactctcttt gctgttgctg gggatcacgt tcacattacc ggactggctg ggaacgctgc
     2281 tgattctgtc atcggtgatt ttgatttcaa tggattcccg tcgccgcgcc agaaaaataa
     2341 atcgtccggc gcggcatgag tgagaattac cagccaggca ccgcgccacc gttaaaaatg
     2401 gtttctgccg ctttagcgac ttcgggtgat tgataagatt gcagaaattc cttcacgttt
     2461 tctgcattct tattatcttc ccgtgccacc agaatattca catacggcga atttttatct
     2521 tcaataaata cgctgtcgtg caccggagaa agcccggtct gctgaatgta agtggtgctg
     2581 ataatcgcca catcaacttt gggatcatcc agtacgcgcg ggagctgcgc cccctcgagt
     2641 tccataatct gcaaatgacg aggattatcg gttatatcca gcgcggtagg taataaccct
     2701 tttccctctt tcagagtaat tagcttctct ttttgcaaca gtaaaagcgc gcgaccaagg
     2761 ttggtcgggt cgttggggat cgccactgtc gcgccttctt ttatttgcgc cacggttttg
     2821 atttttttgg aataacccgc catagggaag acaaacgtat ttcccaccgc taccagttta
     2881 taaccatgcg cctgattatc ttgttcaaga aagggtcgat gctggaagac attcgcatca
     2941 agttcgccat gatttgtcgc atcgttcggt agcaacgaac cgctaaatcc taccagttca
     3001 acatcgaggc catatttctc ttttgccacc tttttagcga cttccgcgac atcttgttct
     3061 gcgccattta taacgccaac tttaatgtgt tttgcatcgc tgctactctg gtcgcaacct
     3121 gccagcagaa ttccggccag caataatgcg gcccctgtcc gtagatgatg tgttgtcagt
     3181 ttcatttttt tatcctttta ttaatgatta cctgactata gcggtaaggt cgctgcggtt
     3241 taaaaaacga aacgctatcg ataagaataa aaaggaataa aagtggaata taaagtctgg
     3301 cacttcttac tgaccacgca agcgcgtttc gtacagcatg atgagagcga tgaaagcaaa
     3361 ctacacttat gcttcattcg atacacattt gtaaaaggtt gaatccatga aaccaacgac
     3421 actactgctt attttcactt ttttcgctat gccaggcatc gtatacgccg agtcgccttt
     3481 tagctcactg cagtccgcaa aagagaaaac cacggtatta caagatttac gtaaaatttg
     3541 cacgccacag gcgtcattat cagatgaagc gtgggaaaag ttaatgttgt cagatgaaaa
     3601 caataaacaa catatacgcg aggccatcgt ggcgatggaa cgtaataatc agagcaatta
     3661 ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa gaacagcaac aactactgac
     3721 caacgcttct ttacctgata tcaggatttc ttcattttca cctttgcagt aaccaacaat
     3781 gcagtcagca acatcaatgt gccggacaac atcaacggcg aagtcagacc aatattatcc
     3841 agcgcataac cgccgattgc cgcgccacag gtattagcaa gctgaataac cgccacctga
     3901 atagacccgg ctttttctgc ctgatcggcc agcgagcggg tgatccacgt tgaccagccg
     3961 acgggaacca atgcaaaagt tagcccccag ataatcgcca cgccggtagc aacgatttta
     4021 tcgcttcccc acaacgtcag taccaacgca ctcacagcca gtattaacgg cgcgcctgct
     4081 aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa gcgacgtacc aataaagctg
     4141 gcgataccaa aactcaacag caccagcgtt aagccatcca cgccgaatcc cgccaggttc
     4201 atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc cggcgaaaga catgaagatg
     4261 gcgatcatcc ctgccatcac acccggacgt tgtaataagc ggaaagtatt ttgtttctga
     4321 tgcgagggtt cgcctggcag tgaaggcaat gatttgataa tccagaaaat acacagcacg
     4381 cccatcaccg ccgccgcatt aaagacattg cgccaaccga taagctcgcc taaaaaactg
     4441 cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc cgaagatcac cgacagcgcc
     4501 ttcggcaccg tacgcggcgg taccagacgc atggtcagcg acgccgacat cgcccagaac
     4561 ccgcccagcg ccagtcccag acaggcacga ccgattaaaa gcaaactgaa tgagttagca
     4621 aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa acaaaataac aacgtagcgg
     4681 cggtcagtgg cctgaattgt ctgggtaata aacaaactgg caaacattgc cacaaaggcg
     4741 gtcacggtca ccgattgccc ggcaacccct tccgaaatgc ctaaatcctg ggccattggc
     4801 gtcaacaaac tgacgggcaa aaactcaacg ataatcagac aggcgacaca aaacgccacc
     4861 gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg cgccgcggtt ttcggcaata
     4921 aattcactca tggtgttacc cgtggcatgt ttcaggggaa aagccgacca ctttaacatt
     4981 gaaatgtgtg acgcatttaa cgtttttgca acttttacgg tgttgcttga gctggagtta
     5041 agatcgaaac ggaacagggc aaagattacc ggatgcggcg tgaacgtctt atccacccga
     5101 caggtcaggc gcggtctgta ggctgataag acgaagcggt tttaatgcag ccagtgaacg
     5161 ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa aagcattaat atcctgcatt
     5221 tcaggttcgg tttcgcgcag aaaacccagc gccagcctga cctgagcatc ggtaatcggc
     5281 gcggcaagac gggctttttg taaaatccga tgccattgca gcaggttctg ttcgctacca
     5341 tcgacaatga caatttgcca gatgagcaac acctgcgcgg cgttttgcag gtgcgactca
     5401 tccgggcgat gcaaataacg taaaaattcg tcaccggctg actttcccat ttgatcgacc
     5461 atcgactcca cgggtacgac atcgatattc agccgatcgc tgagacggcg aatcgctttt
     5521 ctggccccgg atgtcagcac ccgaaaacca accacaaaca ctaccgccag cgtggcgagc
     5581 attatccaga tcatgagttc tcctgcgtaa tcagaactca tgatatctgg aaaccctcgc
     5641 cagttactaa tccaccagaa taatcttcag tgcaaacaga gctgcgacca ccaccacaca
     5701 caggttcaga tcgcgccagc gcccggtgca cactttcatg atgcagtacg acataaagcc
     5761 gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc atcaccgtgg taataaacgc
     5821 aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc aggctcgaag tcatcagcac
     5881 gccaacaaag attaacgctc cggcggttgc gtaaggagga accatcgcca ccagcggtga
     5941 gaagaacata accaacagga acataacgcc aaccacaacc gcagtcagcc ccgtgcggcc
     6001 accgactgcc acaccagaag tactttcaat ataggcagta acagacgagg tgccgataaa
     6061 cgcacccgcc accgaactga cgctatcaac atacagcgcc ttattcatat tggggaattt
     6121 accgttacca tctattaagc ccgctttatc agttacacca attaatgttc ctgatgaatc
     6181 aaataggttg atcagcataa aggagaaaat gataccggcg agttcaagtg ttaacgcgcc
     6241 gctcaaatct acttcaccaa tgacgccgct aatatcaggc ggaatggaat agacgccgct
     6301 aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc accacaatag aaaccagcac
     6361 cgcggcatga aaatgacgtg atgacaacac ggttataata aaaaacccta aaatacctaa
     6421 caacacgccg tgagaactta aatcgccaat catcaccagc gtgtctttat tggcgacaat
     6481 aacgccagta ttttttaatc ccattaaggc gataaataat ccaattccgc tggtgatacc
     6541 aatacgtaaa cttaagggaa tgttggagat catccagtac cggatacgaa acagcgtgag
     6601 caaaaatagt ccaactgcgc cccagaatat tgcgcccatc ccggtctgcc aggagatgcc
     6661 catcgccccc acgaccacga aggcaaagaa ggcgttcagc cccattgccg gagccagcgc
     6721 cacgggtaag ttagcaaata tccccatcgc aatactgccg ataccggcaa tcaaacaggt
     6781 ggtaacaaac accactttcg ggtccatttg tgccgcgccg aggatttgcg ggttcacaaa
     6841 aacgatgtac accatggtta aaaaagtggt catccccgca atcaattctg tgcggacggt
     6901 ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc cctgattcat tactcacgta
     6961 atcggtattg tcattattca tttttttgtc cattggagga gatttaatcc cttgcttttg
     7021 taaaacaaat gacaacaacg gttcagtgat aattatcaca tttcaattgc acattaatgg
     7081 atattcttta ataatctcgc gacgtttctt tatgataaat aataatcaaa ttgataaaat
     7141 caaaatgaga aaaatatgaa taattctatt aaccataaat ttcatcacat tagccgggct
     7201 gaataccagg aattgttagc cgtttcccgt ggcgacgctg ttgccgatta tattattgat
     7261 aatgtctcta ttctcgacct gatcaatggc ggagaaattt ccggcccaat tgtgattaaa
     7321 ggacgttaca ttgccggtgt tggcgcagaa tacgctgatg ctccggcttt gcagcggatt
     7381 gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg ctcacctgca tattgaatcc
     7441 agcatgatga cgccggtcac ttttgaaacc gctaccctgc cgcgcggcct gacgaccgtt
     7501 atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag ccggattcgc ctggtttgcc
     7561 cgctgtgccg aacaggcaag gcaaaaccag tacttacagg tcagctcttg cgtacccgcc
     7621 ctggaaggct gcgatgttaa cggtgccagt tttacccttg aacagatgct cgcctggcgg
     7681 gaccatccgc aggttaccgg ccttgcagaa atgatggact accctggcgt aattagcggg
     7741 cagaatgcgc tgctcgataa actggatgca tttcgccacc tgacgctgga cggtcactgc
     7801 ccgggtttgg gtggtaaaga acttaacgcc tatattactg cgggtattga aaactgccac
     7861 gaaagttatc agctggaaga aggacgccgg aaattacaac tcggcatgtc gttgatgatc
     7921 cgcgaagggt ccgctgcccg caatctcaac gcgctggcac cgttgatcaa cgaatttaac
     7981 agcccgcaat gcatgctctg taccgatgac cgtaacccgt gggagatcgc ccatgaagga
     8041 cacatcgatg ccttaattcg ccgcctgatc gaacaacaca atgtgccgct gcatgtggca
     8101 tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc tgaatcacct cggcttactg
     8161 gcacccggca agcaggccga tatcgtcctg ttgagcgatg cgcgtaaggt cacggtgcaa
     8221 caggtactgg tgaaaggcga gccgattgat gcgcaaacct tacaggcgga agagtcggcg
     8281 agactggcac aatccgctcc gccatatggc aaaaccattg cccgccagcc agtttccgcc
     8341 agcgactttg ccctgcaatt tacgcccgga aaacgctatc gggtcattga cgtcatccat
     8401 aacgaattga ttacgcactc ccactccagc gtctacagcg aaaatggttt tgatcgcgat
     8461 gatgtgagct ttattgccgt acttgagcgt tacgggcaac ggctggctcc ggcttgtggt
     8521 ttgcttggcg gctttggact gaatgaaggt gcgctggctg cgacggtcag ccatgacagc
     8581 cataatattg tggtgatcgg tcgcagtgcc gaagagatgg cgctggcggt caatcaggtg
     8641 attcaggatg gcggcgggct gtgcgtggta cgtaacggcc aggtccaaag tcatctgccg
     8701 ttgcccattg ccggcctgat gagcaccgac acggcgcagt cgctggcgga acagattgac
     8761 gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg atgagccgtt tattcagatg
     8821 gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa ccagtcaggg gctatttgat
     8881 ggcgagaagt ttgccttcac tacgctggaa gtcacggaat aataaaaaag cccggcgtca
     8941 tgccgggcaa aagtcaccag ttacgtttat gccactgtca actgctgaat ttttttctcg
     9001 cggcggattt tgcgttcttc catcaccgcc actatcgcca tcaggcagat acaaccaatc
     9061 gcggcgatat caagtgcggc gaatgtgcct gcccagccgg taaggccgaa taccggcgtc
     9121 ccatcggcaa tcattcccag acctaacttg gcaaagctgt caccaatcag gtaagcaaag
     9181 gtgcctttaa taccatcggc agcgccaatc gcttttttag gtacaaagcc aacagcagcc
     9241 acaccaatca acaattgcgg gccaaagacc aggaaaccca acgcaaagag agaagccaga
     9301 tagatatatt cgttgctggc atgttgatac acaccgagcg tggcgataat cagcgccagc
     9361 gcgatgcagg ccaccaggcc acggcgaccg ttcgccaggt cagagagcca gccccacagc
     9421 agcgtaccga ccagcgcacc agcttcaaac agcgtaaagc cctgaatcgc caccgcttta
     9481 gagagtttca gttcctggaa cgcgtatacg gttgaccact ggtcgatacc aatacgtacc
     9541 acatagagga aaatgttggc gaagcacagc agccagatca ctttgttttt cagcacatac
     9601 tcaacaaaga tctgccactt ggtcatatcg gtagattctg tctctttgtc ctcttcgctg
     9661 atctcctcgc cgaacagttc ttcagctttg ccgaggccat aagattccgg ggagtcgcta
     9721 ccgtaacgca ggccgataaa accgacaatc agcgcgataa tcgacgggaa gataaacatg
     9781 ccgatgacat ggccatcgaa caggtaattt gccccgaaca gcgccacacc tgctgcgcct
     9841 gcaccgccaa ggttgtgaga aatattccag aaaccgagga atgtcccgcg tttacgacgc
     9901 ggcgtccatt tggtgatggt ggagtaactg cacgaaccgc cggtactctg gaaaaagccg
     9961 cttaaggcgt agaaggcaat catcaggaac aggctaaccg agccgctgcc catactggca
    10021 ctgaagccca gcatacaaat ggcagacagg ataagcataa acggcaggaa ttgtttggtg
    10081 tttttgccgt cggcgtagta ggaaaccagc gttttaccca cgccataagt gatggagaaa
    10141 cccaggccga tcatccccag ctgcgtcatg ctcaacccgt aggtcgaaat catgtcgttc
    10201 tgcgcgatgt taaagttctt gcgaatcagg tacatcgtca ggtagccgat aaagaccacc
    10261 aggtaggatt gcatgaacgg tttgaaccac attttgcgcc gcacttcgag cggaaggtcc
    10321 agggtcggct tgcgaacctg gtttaagaaa gccagcatgg gttactcctg aaatgaatac
    10381 ctgcgtgagg catgcattgt aaaaatcagc agcaaatcag gcctgagata gcgtccaggc
    10441 aaaacctgga aaatttctta gttttgccgg atatgaagtg aaaaggtgag atgcatcacg
    10501 cttcgcgcgg tgtctgggcg ttcaaaaagg gcagtaacag cagtgcggaa atcccggcgg
    10561 cgatagagat aaccacaaaa aatccgctcc agtgccaggt atcgagtact ttcgccagcg
    10621 gccaaccagc aagcgacgcc cccagataag caaacaagcc gacaaacccc gtcgccgccc
    10681 ctgccgcctc tttgtgggaa cactctgccg ccgccatacc gattaacatc tgtgggccaa
    10741 agacaaaaaa accaatggtg aagaagcagg ttgcctgcat cacgtagctg gcaaatggca
    10801 tcagccacag cgagccgact gaaagcaaaa ttccggcggc gaaaatcaaa ttcatcggcc
    10861 cacggttgcc gttaaacaat ttgtccgagc cccaaccggc taccagcgca ccgataaatc
    10921 cgcccagttc aaacatcgtc actgccgtat tcgccgtgac cagatcgacg cccagcgtct
    10981 cggacatata caaattgccc cagtcgttga tcgccgcccg gaccacatag accagcacat
    11041 agcaaaacga aagcagccag atatacggat tcagcaacac atatttggtg aggatctctt
    11101 tacgcgtcaa ccctgcccct tcttgttgtt gagcaatttc cagcgcgtcg tgccgccatt
    11161 caccgaccgc tggtaaacct aacgcctgcg ggcgatcgcg tagccgccag cagagaaaaa
    11221 tccccacgac tatcgccata caaccagcaa tcatcatccc ggcacgccag ccgtaatgca
    11281 gcgcagccgc tgccatcaca atgggaatga gtgcgccgcc gacattatgc gccgtgttcc
    11341 ataatgccca ccaaccgccg cgctcggtac gagaatacca ggcggttaac agacgcgcac
    11401 acaccggtga accccagccc tggaaaaagg cgttcagcac ccagagcacg gcaaacgccc
    11461 atagcgacgt cgagaagcca aacagaatgt tgatgatgcc agtggcgata agccctatcc
    11521 ccataaaata acgggcattt gagcgatcgc tgacaatgcc ggagacaaac ttcgacacgc
    11581 cgtaagtaat gtaaaacagg gtcgctaata ggccgatatc gctacggctg agcacgccgt
    11641 tagcaaggat ttctggtacg gcggcgttaa aacttttacg cgtgaagtaa aacagcgcgt
    11701 aacccagcca gatggtcagc agaatatgcc gacgccagta gcgataacgg gcatcaattt
    11761 catgtttatc agtcattaat ggcgcatcgg caggcgcttt cagaaacggc aacatcatcg
    11821 aaccttagac ataacgttga ggtagagaaa cgctgacacg cgtgccgtgc agacaggaaa
    11881 tgtgtaatgt gccacccagc gccgttacgc gctcgcgcat tccggtgagg ccaaaacctt
    11941 gttgcccgga acccggcggc aaaccgctgc catcgtcttc aataaccagc atcaaccgtt
    12001 catcctgctg ccagccttgc agggtgaccg cgctggcatc agcatgtttc acaatgttgt
    12061 tcagcccttc ctggcagaca cgaaacagcg tcacgcgctg gttttcgctt aacgctgatt
    12121 catcgattcg ccattcgaga tggctgacaa taccgcgccc ttccagctcc atttcccgca
    12181 tcagtgagcg gatggccttc tccagggtga gatcatccaa ctggcgcgga cgtaaccgac
    12241 ccaacaaacg gcgcaccgcg tcgtaaacgc ccagcgatag ttgttcgatg agctgcccgc
    12301 tctgcttcac gctggcgtta tctgccgcca gccgctgaac aatgcccgcc tgagtacgaa
    12361 tagcagtgat ggtctgaccg atatcatcat gcagctcacg cgccacatca cggcgcacgc
    12421 tctcttcggt ttccagcaac cgttcagcca gatgctgatt gcgcgccagt tccttttgca
    12481 gcgactggtt aagttcacgc aaccgctgga tgccagcgcc aagcaacaac cctgtcagac
    12541 tttgcaccag cagcgagagc aataaatcca ccggatgatc gcgccaggtt tgactggcga
    12601 tcagcgcgat ggcgttcatc aacgtcgcaa tcagcgcccc ttgccaacca tagtgccagg
    12661 ccagcgcgat aatcggcagc gccagacaga atggcgtaaa gcgcgacagt tcgtccggca
    12721 atcccaactg gagccagaga ctgataacaa acagcagcaa gtaccagacc agatgtcgcc
    12781 cgcgccagtt gattggctga gaaaccagtg acgggccgag cggcagccag gtgttattgg
    12841 cgagatagtg ccagaacacc agacatatcg gggccagcgt caggccgcca gttaaagtca
    12901 gcaacagcgc attccacgac tctttgccgt gccaaagcca gggcagcgac tgcaacaacg
    12961 ccgccgccgt taacgccgcc ccctgtaaca gcaaggtgcg ccagtcacgc tgatggcgat
    13021 agcgcgagat cagcgctacg ggcagtaacg tcagtaaact accgatcatc aataacggaa
    13081 aatgggttaa accgaccgcc tgcgttagcc agtaaatcag cagccactcc gcgcccagca
    13141 gcaccggcca gtatccgcgc gggcattgca gcattagccc cagacgcaga ccaaacggaa
    13201 ataacagcac cgccatatca gggcgctcaa ccagatgcag gctgatactc cacaggcaaa
    13261 accatgcggc agagaagata aaaaagcagg caataacggt aattaagcgg gagaacaacg
    13321 tcttcatcac cagccatcaa acatgcggcg cgccagctct acgtcgttac tgacgcccag
    13381 tttttccatc agattggcgc gatggacgtg taccgttttc ggtgacaagc ccagttcggc
    13441 ggcaatctct ttcaccgcca ttccttgcgc cagtttttcc gccacctggc gttcacgttt
    13501 ggttagcggg tcctgacgac cggatgccag tttaatggca atatccggcg tcagataaca
    13561 gccgcccgtg gcaaccgtat gcaccgcagc aatgagttca tccgggctac agcgtttgga
    13621 aagaaagccg cgtgcccccg cgttaagcgc ctgctcaacc agcgcaggac tgtcgtgaac
    13681 ggagagcata atcgtcgcca tacctttcgg cagctggctt agcagctcca gaccggagat
    13741 atcgggcatg gagatatcgc aaatacacac ctgcacaccg cgccccggca gccccgccag
    13801 cgcctcgcgc cccgaaccaa actcggcaac tacctgcaaa tcaggttcca gccccagcag
    13861 ctgcgcaaag ccggagcgga cgatgaggtg atcgtctata agggcaacgg tgatcatggt
    13921 cttgtcctgg cgggtaaaaa aaaacgcgct taccttaacg ataagcgcga tgttgttcaa
    13981 gccttgagcg gttactgaaa aaacaccgcg atcttgttaa acatcgtcgg atcggactga
    14041 ttacgctgca ctttcacgac atcttccagc ttatcgattt ggcttatcat ctgctccaga
    14101 cgctggtcgt cattgaccag tagccagata tggcttttgt cgctgtcctg aatcggcaga
    14161 caaagaatgc cttcaacgtt aaaagcgcgg cgggcaaaaa ggccacaaac gtgggtcatt
    14221 acgcccggat ggttgcgaac ggtgagctcc agaattacgt tgtcatgagt tgtgttttgc
    14281 atggcttatt cccccaccat ttcagtattc gccgcacctg gcggcaccat cgggtaaact
    14341 ttttcttcgg catcaatgcg cacatggatc agcgccgggc cagggcgatt gatgatttcc
    14401 tgcaatgcag cctgcggatc ggcttcgtta ttcaaatcac aggtttcgag gccgaatccg
    14461 gcggcaatct gcataaagtt gattttgccc ggataggtgg cggcaaaaac gccttgctcg
    14521 tagaacagac tctgttgctg atgcaccagc cccagcgctt cgttgttcat cagaatgatt
    14581 ttgacatcca gctgattttc actggcggtc gccatctcct gaatattcat catcaggctg
    14641 ccgtcgccgg agaaacacaa cactttgcga tccgggttcg ccagcgcagc gccaatcgcc
    14701 gcaggcaggc caaaacccat cgtgcccagc ccaccggagg tcagccactg gcgtgggcga
    14761 ttgagcggat aagcttgcgc ggtccacatc tgatgctgac caacgtcggt ggtgataatt
    14821 gcattgtcat cgacacaggc ggcaacggcg ttgatcaggc cgtaatggct taacggatcg
    14881 cacgctttcg ggattggaca cggaaactca cgctgcaaat ccactaccaa ctggtgccac
    14941 tctgcacgcg gttgcgcttc caccagcggg atcaactgcg ccagcacgtc atcaacatcc
    15001 gcctgaatcg ccacgtgcgg ctgcttgatt ttacccagct ctgcacggtc gatatcgaca
    15061 tgaatgattt tggcattcgg acagaactgc tcggttttgc caatcgcccg gtcatcaaaa
    15121 cgcgcaccga gcacaatcag taaatccgcc tcctgcaaga tatagttagt gctgcgcacg
    15181 ccgtgcatcc ccagcatacc cagcgacagt ggatgcgctt ttggcaacat acccagcgcc
    15241 attaaggtca tggtagttgg cagttgcgct ttctccgcca gttcacgcac ccgcgcaggc
    15301 gcattgatca caccaccgcc cagataaagc accgggcgtt tggcagcgtt aatcatcgcc
    15361 gctgcgtcac gaatgctttc ttcgctaaag gcgggtgcgg cggctttttc tgccaccgcg
    15421 ggctgtgctt caatctcaaa aaccgccgtt tgcacatcct taggaatgtc tatccacacc
    15481 gggcctgggc ggcctgattg cgcaatgcgg aaggcatcgc tcatgacctg cgggagttct
    15541 tcgatatgtc tgaccagata gttgtgtttg gtgatgggga tagagatgcc gtaggtgtcg
    15601 acttcctgga aggcgtcggt gccgatcatc gaggcgggaa cctgaccagt gatgcaaatc
    15661 agcgggatgg agtccagccg cgcatcggca atggcggtca ccaggttagt cgcacccggt
    15721 ccgctacagg ccatacagac cgccggttta ccgtcggtgc gcgccattcc ctgagcgata
    15781 aatcccgcgc cctgttcatg gcgagccaga atatggcgga tttgcgtact ttggcttaag
    15841 gcatcgtaaa caggcaggat agaaccgccc ggaatgcccg tcacaatctt aatgccctgc
    15901 tgttccagga aatgaacgat aaattctgcg ccggtaaagc gcttacgcgt cgatgttgtg
    15961 cccgaacttg ccatgctcca gtccttttct tctgggccga ctttccggga ggtgcttaaa
    16021 cgaaaaaccc cgcccggttt gcgccggcgg ggttttggaa tcgtgtgttg ttccagtccc
    16081 tacggcgcat tgccgacgac caccaccaca cgcacgacga ccactgcggc ggatggcgca
    16141 gttggtagta gttttgcgtt gaacatggaa gtagtcattg gggaccttgt tggttttgtg
    16201 tttaacaata tttatacaag cacagcttta caggggagac aatggaaaat ttttcagcaa
    16261 gggaaaattg aggggttgat cacgttttgt actgaattgc agataacaaa aaaccccgcc
    16321 ggagcgaggt ttcgtcagtc gcctgcggct ggtaaccgca aagcacactg tattatgtca
    16381 acactgaaag tatacgtgtt ccgcgcagaa cgcgcaattt cggcacgaat tttgacgtat
    16441 ttagtgcata gttgagtatc gatcacagtt tgcgttttgt ccaaatatta ctgtttattt
    16501 atacagtaaa cttctataat atcactgtac gcaatgtgtt atgcgggggc cgcatcgtta
    16561 cccggcgcac taagtcctgg ctgaaacggg tggtgccgtc agcgccttaa ccccgcgtga
    16621 gcacactgtg ttatgtcaac aagcacaacg tttctccttg agataccgcg tgcacaacag
    16681 ctggcaacag gcagcggaaa ggtacgtcag ctggcagtgc tcctgaacca caggagacgc
    16741 gtatgagcct ggtggatatc gccattctta tcctcaaact cattgttgca gcactgcaac
    16801 tgcttgatgc tgttctgaaa tacctgaagt aattcagatt caagtcgcac caaaggggag
    16861 cgggaaaccg ctcccctttt atatttagcg tgcgggttgg tgtcggatgc gatgctgacg
    16921 catcttatcc gccctaccat ctctcccggc aacatttatt gccgcttttg tttacatatt
    16981 ctgccgctaa acaattcccc attcctggcg tatatctggc taacattcat caatgtgata
    17041 gattcctctc ccgcatttat gggaatgcgt agtgacttat tctaattatt tttataaaag
    17101 catccgtgat aatgaaaagg caaagaaacg tcaatttgtt attgatgttg gtattactcg
    17161 tggccgtcgg tcagatggcg caaaccattt atattccagc tattgccgat atggcgcgcg
    17221 atctcaacgt ccgtgaaggg gcggtgcaga gcgtaatggg cgcttatctg ctgacttacg
    17281 gtgtctcaca gctgttttat ggcccgattt ccgaccgcgt gggccgccga ccggtgatcc
    17341 tcgtcggaat gtccattttt atgctggcaa cgctggtcgc ggtcacgacc tccagtttga
    17401 cggtgttgat tgccgccagc gcgatgcagg ggatgggcac cggcgttggc ggcgtaatgg
    17461 cgcgtacttt gccgcgagat ttatatgaac ggacacagtt gcgccatgct aacagcctgt
    17521 taaacatggg gattctcgtc agtccgttgc tcgcaccgct aatcggcggt ctgctggata
    17581 cgatgtggaa ctggcgcgcc tgttatctct ttttgttggt tctttgtgct ggtgtgacct
    17641 tcagtatggc ccgctggatg ccggaaacgc gtccggtcga tgcaccgcgc acgcgcctgc
    17701 ttaccagtta taaaacgctt ttcggtaaca gcggttttaa ctgttatttg ctgatgctga
    17761 ttggcggtct ggccgggatt gccgcctttg aagcctgctc cggcgtgctg atgggcgcgg
    17821 tgttagggct gagcagtatg acggtcagta ttttgtttat tctgccgatt ccggcagcgt
    17881 tttttggcgc atggtttgcc ggacgtccca ataaacgctt ctccacgtta atgtggcagt
    17941 cggttatctg ctgcctgctg gctggcttgc tgatgtggat ccccgactgg tttggcgtga
    18001 tgaatgtctg gacgctgctc gttcccgccg cgctgttctt tttcggtgcc gggatgctgt
    18061 ttccgctggc gaccagcggc gcgatggagc cgttcccctt cctggcgggc acggctggcg
    18121 cgctggtcgg cggtctgcaa aacattggtt ccggcgtgct ggcgtcgctc tctgcgatgt
    18181 tgccgcaaac cggtcagggt agcctggggt tattgatgac cttaatggga ttgttgatcg
    18241 tgctgtgctg gctaccgctg gcgacgcgga tgtcgcatca ggggcagccc gtttaagcgc
    18301 acgtcaccgc agcatcgtca tcagctccat gggagaacga tgctgcttta tcagatcacg
    18361 catcacccgc atatgcggtg cggagtaaga ataaaacgcc tgatagcccg cacaaagcac
    18421 gctgtctggc gtctcctgcc gagcgtgcgg gcacaggcga tgccagtcgc acgcctggca
    18481 gtcacggcgc ggttgcgggt tttcgctgcc acaccatttt tgtaaggtgg cgtcaaaaag
    18541 ctcaatcgag atacgactga tatctgcccg cacccaacac tcgaacaggg caattaaaaa
    18601 tttcccccac agctctgctg tcaccgaagc ggccgtcaga tcgccccggt catcaaattc
    18661 aagataggga atgagctgta cggagcggat gccccgctgt ttgaaattgt gataaaaatc
    18721 ggcaggttgc agagcatcct tgtactctac aaccagttta tgcctgtcct cttcagcatc
    18781 aatgacctga cttcctgtca tgcgactctc tcaataaata cgataagttg atgtatatgc
    18841 gttacagcaa acagtattgc gagagataac accgcgaggg agatcaaaaa tttgtcacgg
    18901 actacgtgaa attgggaaaa atcgctattc gtgacatcca ttaaattgcg attacgggtg
    18961 tagtgccaga ggatcagcgc cacgatggcg agaatgccaa tcgaaatcca gaataacatg
    19021 cccgcctggt gccagttgtg tttgatagcc aacgccatca acgcgccgta gcccagcatg
    19081 gtacgaaacc acgccagtga tgtgcgctcc ggctgcagtc ctgggtcggc aatgcgtctg
    19141 gctttgcggc tatccggcat acaacaccag tcccataacg atcaccgcca cgaccatcaa
    19201 tattaagcta atgattaata agctgttggt gtagggcagg tcctctttca gacgcatcgc
    19261 tttttcattg cgtaaccagc gcaggtagcc atacattgct aacccgccgg aaaacagaca
    19321 caacaacaac gccagcagtt cacgaatgac gggcgtggcg aaatctggcg caagttgatc
    19381 aagcccaacc cccgccgcca gaaatcctag tgcggtacgg atccacgcca gaaaagtacg
    19441 ctcattagcc agcgagaagc ggtaatcggg cgcttctcca aggcgggaaa ttttcattgt
    19501 tgctcctttg caatttcact aacgctctat ctttagcggt tttttttaag aatttcgtcc
    19561 gcccgtttcc tgatatgctt ttgccatccg ctacaaggag gtcagatggg tatcatagcg
    19621 caaaataaaa tcagttcatt gggcatgctg ttcggtgcca tcgctttaat gatggggatt
    19681 atccactttt cattcggccc tttttccgca ccaccgccaa cgcttgaaag catcgtggcg
    19741 gacaaaacgg ctgaaattaa aagaggtctt ctcgccggaa taaaaggcga aaaaattacc
    19801 actgtggaga aaaaagagga catggatatc gataaaatcc tcgatcaaag cggtatcgca
    19861 ctggccattg ctgcgctact ttgcgcattt attggcggta tgcgtaagga aaatcgctgg
    19921 ggcattcgcg gcgcgctggt ttttggcggc gggacgctgg cttttcatac cttgcttttt
    19981 ggtatcggca tcgtgtgcag cattttgctg atattcctga ttttttcctt cctgaccggc
    20041 ggttcgctgg tttaagcagc ctgaataatg cccgccgggt gatcatcagc gggcattatc
    20101 gtcagaattt ctgttttttc tcctccacct tcactccttg cgtcggtagg ccggtgtcat
    20161 agtcgcgtac cactggcgaa tagccatctt gtgggcgtgg acgaaacgcc cccatccagc
    20221 gcggccgtgc atctttacgc cacggacgca gactccattg gtaactgcgg aacggatcgc
    20281 gaattttgtc catgtaatcc aataaggcgt catgcatttt gctgcgaacg tctgcaaaac
    20341 ggatatcatc gatcaggtta tgcatttcat ttgggtcatt acggcgatcg taaagttcat
    20401 cactggtgaa gaggttgagt accagtttaa agtcatccgt cacccagcaa cgcaccggaa
    20461 taaaaccgcc aaagctgtca tgctcaatct cgtagcggtt aaattccacc atcacgccac
    20521 gtggctcttt cacggcaagg atattttccc ccggcagaat ctctggtttt tcaatatctg
    20581 ccagcgccat cattgtcggc agtaaatcga tatgactgac tggcgtatcg acctgtcgcc
    20641 gctccccttg cggcgaaagg atgatcagcg gaatgcgggt gatgtcgtca tacatcgccg
    20701 ctcctttact gatcagctta tgtgcgccca tcatttcgcc gtgatcggat gtataaataa
    20761 cccacgtatt ttcacgttgc tctggcgtta aggcattgat gacccgtccg atttggtcat
    20821 caacaaagtc attgcaggca aaatagagcg gatggtgata aagcccgcca tcaccgactg
    20881 gcgatggcat cgcctgcgcc cataagcggt gatgttccgg tttgttagcc aggtcatcct
    20941 gtgctttctc gcccagctcg tagtaaaaat cagcgtattt ctctaaatac tccaccggac
    21001 aggtgaacgg gtgatgcggc tcatcatacg aaaccaccat caggaagggt tcctcggcgc
    21061 gcgcgggctg ttgcagaaaa tctaccgccc gattgctgat gcggtgcgcc caggtgaagg
    21121 ttccgtcgat atggttcgcc tgtaaatcct caacgctgtt taggccattg cgccacaggc
    21181 tgatctcttt ttccgtcagt tcgctaaggt agttcgcccc atcgaaccag taatcagcgt
    21241 cccactccgg cggacactcg ccagtgccga aatagtcatg accgtcgaga tgccatttgc
    21301 cgatgtaaca ggtgtgatag ccggcatcct taaagtagcg ccccatagtg gagatgtttt
    21361 tgcctggcgc gacgttgttg gtccacgggc cggactggtt agcgtagata ccggtaaata
    21421 gtccggagcg tgcaggcgta caaaccggtg aacaggtgta ggcggaatta aagcgaatac
    21481 cttccgccgc cagactatca atattttgcg tattcagcgg tttaccgcta tagcaaccga
    21541 ccatattggt ggcctgggta tcggtcatga tgaacagaaa attggggcgt ttcataggct
    21601 atccttcgcg tcgggagtaa aggttacggc tgggtcgtga cgatgccgcc agctgtcaaa
    21661 aatcaggtag acaatcacta cggcggcaat gaaataactg atcatcgcta accagcgcgt
    21721 accgtagccg ccgaattcag ccagcccggc atagacgcca atcatcgcga acaggatgcc
    21781 aattgacgcg atcttgacgt ttctccacgg tttcatgtcc accgcaaacg catctttgaa
    21841 ggtgaacggc gtggcgcgcg gtttgataaa accgatcacc agcattacga ccacgttgat
    21901 gcagaacgta caggccagca catagaggaa atggaagtcg aacttcacca gatagttgat
    21961 ggtgatgtag ctgattatgc caatccccat cgccactttt gccgccagcg ccgggatgcg
    22021 cgggaagaaa aagcccataa tgatgatggt aaccagcggc acgttgtaaa tgccgttgag
    22081 ctgtttcatc cagctataca gcccctgcgg cgcgttggcg atccacggcg cgaccagcac
    22141 cgaaacgata gcgataaaga aaccaaattt gcgcccgacg gtgaccagct gctgcggctc
    22201 ggcattctgg ttaatgatgc gacggtaaat acccatactg aataacgtac tggcgctatt
    22261 cagaaagccg ttgaaggtac tgatcaccgc accaaataac accgcgccga agaaacccac
    22321 cattggcact ggcagaacgt tattgaccag cgtcgggtag gccatgtcgg ctttcggtaa
    22381 atcctgatac agatgaaacg caatcaaccc tggcagcacc agtaccagcg ggtcgagcat
    22441 tttcagcacc gccgttaaca gcgccccctt ttgcccttcc gccaggcttt ttgacgccag
    22501 cgtgcgttgc acgatgccct gatttgtaca ccagtaaaag gtgttcacca gaatcaaacc
    22561 ggtaaatgcc gcgccaatcg gcaagggatc ggtcgggcca ccgattgagt ttaatttctc
    22621 ggcgtgaacg gtggtgagtt gctcaatgcc ctgcataaag ctgcccttgc ccatcgcgat
    22681 caggccaaat accggcacca tcaacccgcc gataaccagc ccaataccgt tgatggagtc
    22741 cgccactgcc attgcgcgca gtccgccgat caccgcatac aaaattcccg ccagaccaag
    22801 caaaattacc agtagccaga tagccgcacc gtgagaaatc tgtagcgatt ccccgacgtg
    22861 aaacaggctg ttgagcgcca acgcgccgga gtagagaaca atcggcagaa agcagacgcc
    22921 ggtggcaatg aggaagcaga agtcgatgat aatacgcgtc gttttatcat aacgttcctc
    22981 cagaaaatcg gggatggtgg caatgccgcg cttcagatag cgcggtaaaa agattagcgc
    23041 gaggaagatc agcgtcaccg ctgaagtcac ttcccagccc atcaccgaca tgccgctttt
    23101 gtaggcctgc ccggaaaggc cgaccagttg ttccgtggaa aggttggtta gcattaacga
    23161 agcggcaata accggcgctt ttagtgaacg tccggcaaga aaatagcctt gttgcgatcc
    23221 ggtatctgtt ttgcggacct tccaccaggt gatcaccgcc accagcagcg taaaaccgac
    23281 aaaactcaag atttgtaacg aattcatctc atagccctta tttatgttta tgtcccggac
    23341 gcatgaatat agggagaggt aaaatgcgtc gggtcaggca gtcagattct taaaatacaa
    23401 acgtcgtatc cctgaacgga ttgttttctg ttaagttcag gttgtgagca taattcgccg
    23461 gagggatatt aaaatgaatg gaaaattgca aagttcagat gtaaaaaacg aaactccgta
    23521 caatattccc ttattgatta acgaaaatgt gatctccagc ggaatttctc tgatctcgct
    23581 ctggcatacc tacgccgacg agcattaccg ggtgatctgg ccgcgggaca agaagaaacc
    23641 gcttatcgcc aactcatggg ttgcggtgta taccgtacaa ggatgtggga aaattctttt
    23701 aaagaatggc gaacaaataa cactgcatgg caactgtatt atatttttaa agccaatgga
    23761 tattcactct tatcactgtg aaggtttagt ctgggaacag tactggatgg aatttacccc
    23821 caccagtatg atggatattc ccgttggtca gcaaagcgtt atttataatg gcgaaattta
    23881 taatcaggaa ctcactgaag ttgctgaatt aataacttca ccagaagcaa taaaaaataa
    23941 tctggcagtc gcttttctga cgagaattat ttatcagtgg atttgtctta tgtacgcaga
    24001 cggtaaaaaa gatccacaac ggcggcaaat tgaaaaatta attgccactt tacatgccag
    24061 tctgcaacaa cgctggagcg tagccgatat ggctgccacg atcccctgta gcgaagcctg
    24121 gttgcgtcgt ctgtttttac gctataccgg caagacgccg aaagaatatt acctcgatgc
    24181 gcgtctggat ctggcgctat cgctattaaa gcaacaagga aactcggttg gcgaagtcgc
    24241 tgatacgctc aacttcttcg actcctttca tttcagcaaa gcctttaaac ataaatttgg
    24301 ttatgcgccg tcagccgtgc taaagaatac ggaccagcac ccaacggatg ccagtccaca
    24361 caattaacgt aactctggcc agtacgcttt gttggcctcc agcaattcat ccagaatagc
    24421 tttggcgacc gaggcgctcg gtaccgtttt cgacaacgta atcgcctgcc acaggtgctg
    24481 atatgaacgc tgttcccagg cgtccacgac cagtttttcc accgccactt gctgactcat
    24541 taaccctttc tgaaactgcg ggatatcgcc gaccaccagt ggttctggtc cgttgtgccc
    24601 aaccagacac aggatctcga ccatcgcttc atcatcaaag ttatgaattg ccccgttgtt
    24661 aggcacaatc agcaacatcc gctcctgagt gttgaaggca attgccgccg ccagatcgac
    24721 gatgtatgac gcatgttcgt caatttccag cttgcccgcc gctgaatttc ctgccgccgt
    24781 aatggcgcgg caagcatcga aaacctgttt ctcgcgatgt tccatcacct cattcgcgcg
    24841 ggtatgttca gggttggaat gctgaacgac gtaatccggg aagagataat atttcagata
    24901 ggtgtttggt aatgtatccg gggccagtgc ctggacatcc cgcgcttttg cataggtgtc
    24961 gttccagctc gcttcagtat gcgtatcttg ctgcggtgga acataaccat gcttagagac
    25021 atattgccgc agctggggca tcaggtcgtt gccctgcaaa tcttcaattg atgtccacca
    25081 gccaaagtgg ttcaggccgt agtagcgcac gcgcatctgt ttgcgatctt gcagcccaac
    25141 aatttgcgcc atccggcttt caataccgat tggcatgtca cagatgttga ggattttcgc
    25201 attcgggcgc agacgacgcg tggcttctgc gacaatggct gccgggttgg agtagttgag
    25261 catccaggca tttggtgaat atttttccat ataatccacc agttccagga cgccgccaat
    25321 ggaacgcatg ccgtacgcta ttccgccggg tccgcaagtt tcctgaccaa caacgccgtg
    25381 gcgcagcggg attttttcat ccagttcgcg catcgggtat ttgccgacgc ggatgtgcgc
    25441 catgacaaaa tcaacgtcgc tgaatgccac ttcaggatcg gtggtgtaac taaacgcaat
    25501 gtccggcgct ttttctttaa ggatgacttt acaggcttcg gcaatcactt cctgccgcgc
    25561 accatcgtta tcataaaatt tcagtgcacg aagcgggaaa cggtcctgat tcgccaggag
    25621 catcaacacg atgcctggcg taaaggtgct tccaccgcct gcgacaacca ctgagaattt
    25681 ggtcattata ctgcctccgt aatggcaaca ttttctgctg attgatgaga attaattaag
    25741 ctatcgagct gttcacgcag ctgagataca tgcagaccaa tgatcacctg aatggcatcg
    25801 ccactacgga agacgccgtg cgctcccagc tttttaaaga cttcgtcatc cagcgtttgt
    25861 gacatgtcat gcagtgcaat acgtagacgc gtcgcgcaat tgttgatgct ggagatattg
    25921 ccgaccccgc ccagggcttg caggataccg gcagcctgat ccaactcttt ttttggctct
    25981 gcagcggtgg tttggcctcg cgaggcttta tattcggctt ttgagtagag tttcacttcc
    26041 gcatcttcac gtcccggcgt acacatgttg aactgcagta tcagggtgcg gaaaaccacg
    26101 aagtacagca gggtaaagca caacccaatg gcgatttggg tcagcatcat atccgcgtgg
    26161 ttgctgaaca tcgggatcca gttttgcggt aaaacctggt caatcagacc tccgcccatg
    26221 ttgcccacca caccaaagag atacattacg gtcgacattg aggccgcaag cacagcgtgt
    26281 accgcaaaca gcaacggtga aatgaacagg aaggtaaatt ccagcggttc ggtaattccc
    26341 accagcatgg cggttaaggt tgcggggatc agcaagcccg ccacttttac ccgattttcc
    26401 ggtgcggcag tgaagtacat cgctaaagaa atgcccacgg caccaaagat ttttgagtta
    26461 ccgtgcaggg caaaaccgcc ttccgggaac aacgatttca gcggctcggc gctcagactg
    26521 aactcttgca gatgctgcgc ccagtacatc tgaatgccgc cttcaacagc tgccggacca
    26581 aagataaacg gtccgtagat gaagtggtgt aaaccggttg ggatcagaat acgttcgagg
    26641 aaggtgtaaa cccacacccc aagcgcgccc gccgaacgca ggaacgcttg cagagattca
    26701 atccccattt gtacttttgg ccagccgagc aatgtcagcc aggcacaggg gatcatcacc
    26761 aggaaggcga taatcaccac ataagacgtc ccctggaaaa tgccgagaaa caccggcagt
    26821 tttttatcga acagacggtt atgcagcgcc gtcacaatgc cggaaatgat aattgcgccg
    26881 ataatgctgg tatcgagggt tttaatcccg gccatcattg tcagaccgct acctgccacc
    26941 gcgtcctgag tgaaatcgac gccgaagtag cttccccagg tcattcccat cgcgttgatg
    27001 aaatagttcc aggtcaggaa actcaccatc accgccagac aagcacgccc ctgcgcttgc
    27061 ttagcaaggc caatgggtaa accgacagca aaaatcagcg gcatagtacg gaataccgtc
    27121 caaccgccct cttcaataat gtgtacgatt tgcgcgaata aactgttcgg atcggtcagt
    27181 gattccccga caaacatcgg gttttgcagc aagatggcaa gacccaccac aatcccggcg
    27241 aagggaaaca gcagcactgg cgtaaacatc gcgccgccaa agcgttgaat ttgactgagc
    27301 atttccacat cctcttttct caattctgta agtaggaagc ctgattggtt atgcggcagg
    27361 cctggagcga ggataggaag atgcgatcgg cagaacatga cgacccaatg ggattcgtga
    27421 tcgctttcat gcttttcgtt gggcggtaga gatctacgtt ttcgcgaaaa agtagacatc
    27481 tgtgagcact ggtacacagc atcgaacaca tgtttaaaaa aaagatgttt tttcaatcga
    27541 ttaagcagaa cttgtgggcg cattacccgg gcttgcaggc aaaaaagaga tctagagatg
    27601 atctacaaaa gcattgcgga gcggttaaga attcgactta attccgcaga tttcacgcta
    27661 aatagccttc ttcccggtga aaaaaagttg gcggaagaat ttgcggtatc gcggatgacc
    27721 atccgtaaag ccattgacct gctggtagcg tggggattgg tggtccgccg ccacggcagg
    27781 gaaaaacggt caccagccag gtgctgattt ttgaaatcat gcctgcgcct ccggccattg
    27841 ccagccagtt acggattcaa atcaacgagc agatctactt ctcccgtcgc gttcgttttg
    27901 tggaagggaa accgctgatg ctggaagaca gctatatgcc ggtaaaactg ttccgtaatc
    27961 tttcgctgca acatctggaa gggtcgaagt ttgaatatat tgaacaagag tgcgggattt
    28021 tgattggcgg taattatgaa agcctgacgc cagtgctcgc cgatagactg ctggcgcggc
    28081 aaatgaaggt agcggaacac acgccactgc tgcggatcac ctcgttgtca tatagcgaga
    28141 gcggggagtt tttgaattat tcagtgatgt tcagaaatgc cagcgaatac caggtggagt
    28201 accatttacg gcgactccac ccggaaaaga gttaaccgat actccagaag agcaccgcca
    28261 gtaattgggg ggtgataatg cgcaggaaca ttaccaacgg atagacagtg gcgtaagaga
    28321 gcgccgccgc accgctggtt ggatgaagat tattagcaaa cgccagcgcc ggaggatcgg
    28381 tcatggaacc tgccagcatc ccgcacatgg tcaggtaatt cattttggct aacatccgcg
    28441 ccagaatgcc aacagtaatc agcggaacgg cggtgatcag ggcaccataa ccaatccagc
    28501 ttagcccttc gccattgacc agggtattca caaaatcccc accagatttc agaccaacga
    28561 ccgagaggaa cagcacgatc cccagctccc gcagcgcgag gttggcgctt ggcggcataa
    28621 accagtacag cttgccgata ctgccgatac gcccgaggat caacgccata atcagcggtc
    28681 cgcccgccag ccccagtttc aacgcggccg ggaatcctgg cacaaagacg ggaatagaac
    28741 ctaacaatac gcctagcccg atgccaataa acactggcag catctgaacc tgttgcagtt
    28801 tttgctgcgc attccccagc acattggcaa cggcatcaat tgcggacgga cgccccacca
    28861 gattgaggat atcgccgaac tgcaggctga tatcgccact ggcgaccagt tcgaccccgg
    28921 cacggttcag gcgcgagata acaacgtcat agcgttcttt aaaatgcagg tcgcgaatac
    28981 gcttaccaag cacgttttca ttggtgacca ccacacgctc gacgcgcaaa tcagtgcctt
    29041 tcgtggacag cgaggtatcg acctcctgac caatcaccag ttgcgcatta tgtaaatccg
    29101 ctggctgccc caccagatgc agcaaatcgc ccagttggat aatggtatct ggcgaaggta
    29161 cttttagggt ttcttctcgt ttcagacgcg agcagataat tttgtcgccg ttgagaatcg
    29221 gtacatcttt aatggctaaa tcatgcaggt tagggttctc aacgcgaata ttgatagtct
    29281 tgatcagcgc gccgccattg gtgcgtgaag actcgtgctg ctgggcttct gtctcgacat
    29341 tgacgcggaa aataacccgc aacatccaca tggtgaacaa tatcccgcaa atgccgaatg
    29401 gatacgccat cgcgtaactc atccccatct gatcgaccat ttccattggt gtacccaagt
    29461 cgcgcaagat ctgctgccct gcccccagcg ctggcgtatt ggttaccgca ccggagaaaa
    29521 tccccagcac taccggcagt ggaatatcaa acagtttatg caggatggcg gtaaccagac
    29581 caccgatgat gacgatcaga acagcaaaca ggttgaggcg taatccggag acgcgcaatg
    29641 aggcaaagaa gcccggccct acctgaatcc cgatagtata aacgaacaga atcaggccaa
    29701 attcctgaat aacatgcagc atatcgctac ttaatgtcat ccccgcctga gaaacaaaat
    29761 ggccgacgat gatcccacca aacagcacgc cgccaatacc taatcctatg ccgcgaaatt
    29821 tgacgttgcc gataaacaaa ccgacgactg ccaccaaagc caaaatactg accgttaatg
    29881 ctatatcact catcgtccat ttctcttgag ataacatttt aattgctacg gattatggct
    29941 gaatcggtga agcagatttg ccttatagcg cacattatca cgggaaagaa cgtgccgaaa
    30001 tatcttaaac agtcgaaatt taacgcattg ttgaagaaga aagacaaaaa aagccccacc
    30061 agaatggcgg ggcaaagaga atagctagtt agctatttaa cgcgggacgt tcgctgatag
    30121 cgatacgctg cgctgcgatg ggttcaggct cattacgaat taaatcaata tgcagtaaac
    30181 cgtttacgaa ggttgcgcca gagacttcca tattttcagc cagcgtaaag ctcaggctaa
    30241 atggctgatt cataagccct tgatgcagcc attttttctc ttcttttggc tgctccggcg
    30301 tgccttttac gctcaggcgc gtaccttcca gttgaatctc taaatcttcc tgacggaaac
    30361 ctgccagcgc aagggtaatg cggtagtggt tatcgtcgct tttctcaatg ttgtacggcg
    30421 ggaagctctg actttcaccg gcgttttgca gtgcgttggc cagtttgtca aaaccgatcc
    30481 attgacgcat cagtggggat aaatcgaagt tacgcatagt catttctcct tctaagaagc
    30541 gagtaagtac ctgcaaatcc gaagattcgc atatgctccc tgacggcgag catggagatg
    30601 tcaggccgcg ccaggcggcc ttagggaatt agttgatttc gatacggcgc ggttttttcg
    30661 cttccggaat cacgcgttcg agatcgatat acagcaaacc atttaccagg ttagcgccac
    30721 gaacatgaat gttctcagct aactggaatt tgcgttcaaa gttgcgttca gcgatgccct
    30781 ggtacagata ggtgcgctct ttttgttcgt cggcgtgagc acctttcacc accagcagat
    30841 tatcctgggc ggtaatttcc agttcgctct cagcaaaacc agccacagcg atagcaatgc
    30901 ggtaatggtt ttcgtctacc agttcaacgt tatacggagg gtagccgcca ttactctggc
    30961 tctggttgtt ttctaagtgg ttaaacaaac ggtcaaatcc aatagcagaa cggtaaagcg
    31021 gggataaatc aaagttacgc ataatcaata gctcctgaaa tcagcgagaa tgtaagacct
    31081 tccacaatgg acaggtcagg tagccagaac acccatcagg cgcgtcctca tcggctacga
    31141 tgtaaaaatg ggtctgaaaa tgaacttttc aagccttatc agcggacttt tttttgcagt
    31201 ttatggtcta ttgcatagac tgagggggca gcacgaaacg ttaaagtgcg attgccgcca
    31261 cagagcgacg taatgatgat gttattttca gcaagggatc gctataactc atcaggccaa
    31321 cacccacgat caacagatga atttattatg ataagaaatg tgttgttagc gttcatgata
    31381 tgcagtggaa tggcattact cggaggatgc tccagcgtga tgtcccatac cggagggaaa
    31441 gaaggaacgt atcccggcac ccgcgccagc gccaccatga ttggcgatga tgaaaccaac
    31501 tggggcacta agtcgctggc gattctcgat atgccgttta ccgctgtgat ggacacgctt
    31561 ttattaccgt gggatgtgtt ccgcaaagac agctcggtgc gctcacgcgt tgaaaaaagc
    31621 gaagccaacg cgcaagccac caatgcggtt attccaccgg ccagaatgcc tgataactga
    31681 tttagcgatc agtttcggtg atccacagct ggccgccttc catccacgtc agccattgac
    31741 catccggcga gaagaccacg gcgtccgcag aaggtggatt ggcgtggttt tcggttaaat
    31801 actcaacctc gccgctttgc gcatgggcac aagcaattcg attatccagc acaaagccca
    31861 accattctcc tgatggatgc cagttaaatg cagactgaat atccgttttg ttatgggtta
    31921 actggcgcgg ctcgccgccc tgtggcgaga taagccacag ttgcacaatg ccgttatcat
    31981 ctcgcattaa aaacgcgatt tgtgtaccct gcggattaca gcgcacccag tggcgcggga
    32041 cgttgactaa ccccggataa gcccgatggt gggtaaaggt taaacgtcgc tgcacgacgc
    32101 cacgcggtgg cgcgggcagg gttgtttccg ttccacttaa cggcgcatca cctgctgctt
    32161 tccagccagc ttcatcttgc ggtaactcaa cgataaacag ctccggcact ttctcgccct
    32221 ttggcgaaag tgtgtcgcca ataaacgcca gcgcgtgatt tcctacccat ccttcttcat
    32281 aagcacgatt gatttcatcg ctgcccggct gcggcgtggg cgtggttttg ctcaccagca
    32341 cacaccagtg gctcccgctg tattcacgcg gatgctgctt ttgtacattc accggaccaa
    32401 acggtgcggc aacaccgacg tttcgcaaat ccagtgccga atcgagctga tgcattacat
    32461 ggtcgttata ggtaaagctc accctttcac cgtccgggct aaagacatgt acatggctgc
    32521 cgccgcgcag cgctcctggg gtgtacggtg cagtaatatc cattgcatcg agattgctca
    32581 ccttgccgcc tttagcaatg actccgcgtc gatgatggaa atcgtaatgc catgtttcat
    32641 caggattttc agggccgtga ataaagacat atttatctga cttcggatga acggtcacca
    32701 cgccgacatg tgcgccctgt gacgcgcgat agataacctc gacctcgccg gtatggatat
    32761 tcacacgctc aatggtctcg ccggtaaacg acgccccaga aggacgcacg tcaaatacca
    32821 gccactggct gtcgggcgtc caggtattgg tattggtgag caggtgatta cggggagcaa
    32881 aggttatctg tttcatggcg tggtcctgat acaaaacggt tcccgccatc atacttcaca
    32941 aggacttcac tttcagacaa tagtcttttg ccatcgtgat gaatgtgagt gaaaagatgg
    33001 aacatttcga cgtggcgatt attggcctcg gcccggcagg atcggcgttg gcacgaaagt
    33061 tagcaggcaa aatgcaggtg atcgcgctgg ataaaaagca ccagtgtggt actgaaggtt
    33121 tcagcaaacc ctgcggcggt ctgctggcac cggacgcgca gcgatctttt attcgcgatg
    33181 ggctgacgct tcctgtcgat gtgatcgcta atccgcagat tttcagcgtc aaaaccgtcg
    33241 acgtcgccgc atcgctcacg cgtaactacc agcgaagcta tatcaatatt aatcgccatg
    33301 ctttcgactt gtggatgaaa tcgctgatcc ccgccagcgt tgaggtttat cacgacagcc
    33361 tgtgtcgaaa aatctggcgt gaggatgata aatggcatgt catttttcgt gcagacggct
    33421 gggagcaaca tattactgcc cgctatctgg tcggtgccga tggcgcaaac tcgatggtgc
    33481 ggcgacatct ctacccggat catcaaatcc gtaaatatgt cgctatccag cagtggttcg
    33541 cagagaaaca tccggtgccg ttctactcct gcatctttga taattcgata actaactgtt
    33601 attcatggag tatcagcaaa gacggttatt ttatctttgg cggtgcttat ccaatgaaag
    33661 acggtcagac gcgtttcacg acgctgaaag agaaaatgag cgcctttcag ttccagtttg
    33721 gtaaggcggt gaaaagcgaa aaatgcacgg tgctgtttcc ctcacgctgg caggattttg
    33781 tctgcggtaa ggacaacgcc tttctgattg gcgaagcggc aggatttatc agcgccagtt
    33841 cgctggaggg gattagctat gcgctggata gcgcagacat tctgcgttcg gtgttactga
    33901 agcagccaga gaagctcaat accgcttact ggcgcgccac ccgcaaactg cgtttaaaac
    33961 tcttcggcaa gatagtaaaa agccgatgcc tgaccgcacc ggctttaaga aagtggatta
    34021 tgcgcagtgg tgtggcgcat attccacagt tgaaagatta gccaacgcgc ttcacatcgc
    34081 ccaccagcag gatgtaagag agcgcgccaa tcaacgcgac ggcggagata taaaccagtg
    34141 caggtgcgaa accgtaaccc tgcgccaggt accccaccac cagcggaacg gtgatgccgc
    34201 ccagaccacc ggcgaagtta aacacgccac cggttaaacc aatcaggcgc atcggtgcca
    34261 gagaagagac cagcgaccag gtaatcgaag caaaaccgtt gccgaagaat gccagcgcca
    34321 tcaggcacat aatcatcatc ggatcgttag tgtagttagc gcccataatg caggtggaga
    34381 tcagcaagcc gcagataatc ggcgttttac gcgcaaagcc cagtgaaaag cccttacgta
    34441 ccagcagatc cgcgacccag ccagagagca ggacgccgac aaacgccgcg aggaatggca
    34501 ccgtggtcat aaagcctgct ttcagcgccg tgattccttt ttcctgggtt aaatagttcg
    34561 ggaaccaggt taagaaaaac cacagtgtag aagccaccgc aaattgccca agatagacgc
    34621 cgatcagttt ccgatggaac accagtttcc agtctttggc tgttaacggc tgacgcgcct
    34681 ctttcttcac cggcgcatca ccatccacca gaccgccgcc atcacgaatg taatccagtt
    34741 cagctttgct gatacctttg gtcaggcgcg gcggctgata aaccttaaac caaatcagcg
    34801 accagataat gccgatacca ccagtgacaa tgaacaccca gtgccagctc aacatctcct
    34861 gaatccagat cagcagcgga gtcagaaacg ccagaccgac aaactgacca gacgtataaa
    34921 aaccaacggc agaagcgcgt tcatgttccg ggaaccagct ggtcaccatc cggttattgg
    34981 tcgggaaagc gggcgcttcg aaaataccgg ttatcgcgcg cagaccaatt aatgacatta
    35041 atcccgaggc aaagccctgg aataaagtcg ccactgacca gccaaatatc gcaataaaat
    35101 aagtcacgcg agaacctacg cgatctaaaa accaaccgcc ggggatctga catagcgtat
    35161 aaagtcaggc gaaggccgaa aatacatagc ccatttgcgc tttggtaata ccgaactctt
    35221 cctgaatatg ggcggaagcc acggccaggt tggcgcggtc gacataacaa atgactaccg
    35281 taataaagat catcaccagc gtcagataac gccgacgccc cggctttgct gcattaacgg
    35341 gaatatccat agcgagcttt ctccagattt tgggcatagc gaagccgctc accatgccct
    35401 gtaatttaca gagggttata tttttgtatt gctgttttag tgcccgatga ggggcttacg
    35461 tggcaggaat taccactctg ctacgctgtt atcttcatga cgccagagcg gattacgcca
    35521 gtccggggca tttttactga actcaatcac tttagcttcg tcgatttcca cgcctaagcc
    35581 cggtttcgtt aacggtttaa agaagccgcc gaccatgctg aagtcttctt tgtttttcac
    35641 aaagtcgagt aactccgcgc ctttgttgta atgaattccc atactttgtt cctgaagtac
    35701 ggcgttatag gaaacaaagt cgatatgcag gcaagccgcc agtgcaatcg gtccgagcgg
    35761 acagtgcggc gcaagggtca cgtcataggc ttctgccatt ccggcgattt tgtagcattc
    35821 ggtaataccg cccgcgtggg agagatccgg ttgcagaatc gaaataccgc ctgcctccag
    35881 cacgcgttta aaatcgaagc gtgagaacat gcgttcaccc gccgccagtg gaatatgcgt
    35941 ttgtgccgcc agtttcgggt agtattcggc ttgttccgcc agcaccggct cctcaataaa
    36001 cagcgggcga tacggctcca gttctttaat cagcactttc gccatcggcg cgctgacgcg
    36061 accgtggaaa tcaagaccaa actcaatctg attgccaaaa gcttcacgaa tttgtgccac
    36121 ggtgttaacc gccgcatcta ccgcgcggga gttatcaatt agccccagtt cttcacaacc
    36181 gttcagtttg aaggtatcga agccgatttc gcgtagcgtt ttaatgccgt cgataacatc
    36241 cgccggacga tcgccgccaa cccaactgta ggctttaatt ttgtcgcgaa ccaggccgcc
    36301 catcagttgc cagaccggcg cattcagcac tttacctttg atatcccata acgcctggtc
    36361 aatcccggcg atggcgctca tcaggatcgg accgccgcga tagaatccgg cgcgatacat
    36421 cacttgccat aagtcattga tgcgcgatgg atcctgacca atcaaatagt cacccagctc
    36481 gtgaactgcc gcttccaccg tacgggcgcg gccttcgatc acgggctcgc cccaaccgac
    36541 cacgccttca tcggtttcaa ttttcaggaa catccagcgg ggaggtaaac gatacgtggt
    36601 aattttggtg attttcattg cactgcctct cgatacgcct taacaaatgc tgctgcctgc
    36661 tgcgcggtgc gctctacgga ttgcccggcg cgatagagat cgctgcctaa gcccgcccct
    36721 gcacaacctg cgtctatcca ctgcgccagg ttttctggcg tcacgccgcc aacggcaaag
    36781 actgcgatgt ccgatggcaa taccgctttt aacgctttga tgtattgcgg accaaaagcc
    36841 gatgacggaa atattttcag cgcctgcgcg cccgcttcga gcgcggtaaa ggcttcggtc
    36901 gccgtcgcgc agccggggca gacggtcatg ccgtagccca ccgcacggcg gatcacttca
    36961 ctatggatat tgggcgtaac gatgagctga cagcccatcc tggcgagcgc atcgacctgt
    37021 tcaggtttca gtaccgtacc tgcgccaatc aacgccttgt cgccgtacgc atcaacgatg
    37081 gcgggaatgc tttgctccca ttgtggggaa ttcagcggga tttcaaccgc gtcgaacccg
    37141 gcgtcaatca ccgcgccaac atgcgccagc gcctcgtcgg gcgtaatacc gcgcaaaatg
    37201 gcgatcagcg ggagtttagt ttgccactgc atgagcgatg ctccttatac cagcctgaaa
    37261 tgccgtgtcg cccgccaccg ccgtcacgtc gcaacccatc gcctgaaagg cttgctggta
    37321 gcgcgcggtc agcgatgttc cggcgacaag ggtgatggcg tgttgatggg ccacatagtc
    37381 gcgcatactg gcgacctctg cgccaatcaa caaaccagag agaaattcgc tgacctgttc
    37441 gcggggaagt gttcccagca catgcgaggc gcgaacttca aaaagctgcg gcaatatggc
    37501 gggcgtatta agaccacgct caaggccagc tgtgaaggca tcggcagagt tttcctgcgg
    37561 cggcaaacct gcgccaatca atgagtgatt taacagtaaa tgatgtaatt caccggtcat
    37621 cacggtgcga aaatcgttga tttgctggct atcggcctgc acccatttgc aatgggttcc
    37681 gggcatgaca taaagagagg aaggagccag agctcgcgcg ccgatcaatt gtgtttcttc
    37741 gccgcgcatc acattgtggt tatcgtcatg agagacacat aatccgggaa taatccagat
    37801 attgtcgcca actgacgtta attgttcgcc aatagacgaa aaacaggcag gaacagataa
    37861 atacggtaca actttccagc cgacgttgct gccaaccatt cctgccatta ccactggcgt
    37921 tttctcttca cgccagtcgg tcgtgacttc tgctaacacc gcagccggag attttccgtt
    37981 caggcgcgtg acgcctgctt ctgattgcct gctctccagg cagtggtcgc cctgataaag
    38041 ccaggcgcgc agattggtcg atccccagtc aattgcgatg tagcgagctg tcatgtgatt
    38101 tcctttaacc ttcgtgtcga gctggcgatc atggtaagcg ccgcctgctc tgccgcatcg
    38161 ccgtcctgat gtcgtatcgc atcgaacagc gccttatgtt cctggagcgt ttgcggcatg
    38221 ttggcctcat cgcccatcca gcttcgttca aaaaccgccc gctgcagcga actgatcgca
    38281 atgctaagtt gctgtaacac cgggttatgc accgactgca gcaccgcctc gtggtagcga
    38341 atatccgctt cgttaaacgc ttcgcggtcc tgattgttgg caatcatctc gttcagcgcc
    38401 gattcaatct gcgccagatc gctggaagtc gcgcgctctg ctgcccaacg ggcaatcgcc
    38461 ggttccacca gatttcgcac ttcgctcatg gcactgataa gccgtgggtc gtagtcattt
    38521 tccagcaccc attgcagtac gtcagtgtcg aggtaattcc actggttacg cggtgccaca
    38581 aacgccccgc gataacgttt catttcaatc agccgcttcg ccatcagcga acggaacacc
    38641 tcacggatga tgttgcgcga ggttgcaaac tcctcacaga gctccgcctc agccggaagc
    38701 ggcgagcctg gcacgtattt gccgtgaacg atctgtttac ccagcgtaat gacaatgcga
    38761 tcggttttat tgagagtcat ggagagtcct tgtgctctgt atgttcttct ctactttacc
    38821 ccgatcgatg cataacgcgg caactttgta gtaccagcgt gatgacgttc gcgtttgccg
    38881 tgcgtgtaat gtagtacaaa cttatattgt tgtactacaa tttagatcac aaaaagaaca
    38941 atacataaaa aaatgacatg cgtcgggcag aaatctgaaa agggatatca ggcgctaaac
    39001 aggagggaaa gaagagtatg ctttcaacag cttagctact cgtttaaagg attaatcatg
    39061 aagttgaatt ttaagggatt ttttaaggct gccggtttat tcccactggc gctgatgctt
    39121 tcaggctgta tctcgtatgc tctggtttcc cataccgcaa agggtagttc aggaaagtat
    39181 caatcgcagt cagacaccat cactgggcta tcgcaggcaa aagatagtaa tggaacaaaa
    39241 ggctatgttt ttgtagggga atcgctggat taccttatca ctgatggtgc cgatgacatc
    39301 gttaagatgc tcaatgatcc agcacttaac cggcacaata ttcaggttgc cgatgacgca
    39361 agatttgttt taaatgcggg gaaaaagaaa tttaccggca caatatcgct ttactactac
    39421 tggaataacg aagaagaaaa ggcactggca acgcattatg gttttgcctg tggtgttcaa
    39481 cactgtacca ggtcactgga aaacctaaaa ggcacaatcc atgagaaaaa taaaaacatg
    39541 gattactcaa aggtgatggc gttctaccat ccatttaaag tgcgatttta tgaatactat
    39601 tcacccagag gcattccgga tggtgtttcc gcagcattac tgccagtgac tgttacgctg
    39661 gacatcatta ctgcaccgct gcaatttctg gttgtatatg cagtaaacca ataatcagta
    39721 agcgggcaaa cgcgtttatg ctgtttgccc gcccacagat taattcagca catacttctc
    39781 aatagcaaac gccacgccat cttcaaggtt agatttggtg acaaagttcg ccacttcttt
    39841 cactgaagga atagcgttat ccatcgccac accgacgcct gcatattcaa tcattgcgat
    39901 atcgttttcc tgatcgccaa tcgccatgat ttcttccggt ttaataccta acacgtcggc
    39961 cagtgatttc acccccgtac ctttgttaac gcgtttatcg aggatttcga ggaagtacgg
    40021 cgcacttttc agcacggtat atttctcttt cacttcctgc ggaatacgcg cgatagcctg
    40081 gtcgaggatg gcgggttcat caatcatcat cactttcagg aactgggtat tggggtccat
    40141 tttctccgct tcgcagaaca ccagcggaat tgtggcaacg aaggattcat gcaccgtgta
    40201 gtagctgata tcacggttgg cggtgtacag cgtggtgcgg tccagggcgt ggaaatgaga
    40261 accgacttcg cgagagagtt tttccaggaa acggtagtcg tcatagctga gagcggtttg
    40321 cgccacggtg ctaccatcag cggccttctg taccagcgcg ccgttataag taatgcagta
    40381 gtcgcccggc agttccatat gcagctcttt caggtaattg tgaacacctg cataagggcg
    40441 acccgtcgtt agcacgacat tcacgccacg ggcgcgagct gcggcaatcg catttttaac
    40501 ggcgggtgaa atggtgtgat cgggtagcag aagggtgcca tccatatcga tagcaatgag
    40561 tttgatagcc atgagttccc caggtagatt agttcctgac ccatgctaac gcgattccgc
    40621 tcaaaaatca gtacaacacc cgagggaaaa gggggatgca acgcgggttg catgctcccc
    40681 atttgcttag cggaagagtt tccctttcag cagttccatg cctgcggaaa gcagatcgtt
    40741 attggcttgt ggagacactt caccttgcgg tgagagcgca tcaataatct tcggcaattg
    40801 ttctgccagt aaactggaag ctgtactggt atccacgcca agtttttgtc caagatcgga
    40861 caccgcattt gtgccgagcg ccgattccag ttgctcgcca ctaaccgatt gattgccctg
    40921 ttgattactc agccaggttg agagaatggc ccctaagccg ccactttgca gtttttccag
    40981 cagcacctga atgccgccct gctcctcaac ccaacttaaa atagcctgat atttccccgc
    41041 atcgcctttc agaaaggcac cgacaacttc atcaaaaagc cccatgataa tcacctgtaa
    41101 agcgttacgt gttgacccaa aaagtataga tttgcgggat gataattgcg gattgcagaa
    41161 ataaaaaggg cggagatgat ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg
    41221 aacgccttat ccggcctaca aaatcatgaa aattcaatac attgcaagat tttcgtaggc
    41281 ctgataagcg tagcgcatca ggcacgctcg catggttagc gccattaaat atcgatattc
    41341 gccgctttca gggcgttctc ttcaataaac gcacggcgcg gttcaacggc gtcgcccatc
    41401 agcgtggtga acaactggtc agcggcaatc gcatccttaa cggtaacgcg cagcatacga
    41461 cggctttccg ggtccatggt ggtttcccac agctgttccg ggttcatctc gcccagacct
    41521 ttataacgct ggatggagag gccgcgacgg gactctttca ccagccagtc cagcgcctgc
    41581 tcgaagctgg ctaccggctg acgacgctcg ccacgttcga taaacgcatc ttcttccagc
    41641 aagccacgca gtttctcacc cagcgtgcag atacgacgat attcgccacc ggtgataaac
    41701 tcgtgatcca gcggatagtc agtatccaca ccgtgggtac gcacgcgaac aatcggctcg
    41761 aacaggtttt gttcggcatt ggtgtggaca tcaaacttcc actggctgcc gtgctgttct
    41821 ttgtcgttca gttcgctgac cagcgcgttc acccagcggg taacggtctg ctcatcagag
    41881 aggtcggctt ccgtcagcgt cggctgatag ataagctctt tcagcattgc tttcggataa
    41941 cgacgctcca tgcggttgat cattttctgc gtcgcgttgt actcagatac cagtttctct
    42001 aacgcttcgc cagccaatgc cggtgcgctg gcgttggtgt gcagcgttgc gccgtccagc
    42061 gcgatagaga tctggtactg atccatcgct tcgtcgtctt taatgtactg ttcctgcttg
    42121 cctttcttca ctttgtacag cggcggctga gcgatgtaga cgtgaccacg ttcaacgatt
    42181 tccggcatct gacgatagaa gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg
    42241 tccgcatcgg tcatgatgat gatgctgtga taacgcagtt tgtccgggtt gtactcgtca
    42301 cgaccgatac cacaaccaag cgcggtgata agcgtcgcca cttcctggga agagagcatc
    42361 ttatcgaagc gcgctttctc gacgttgagg attttaccct tcagcggcag aatcgcctgg
    42421 ttcttgcggt tacgcccctg cttcgcagag ccgcccgcgg agtccccttc caccaggtac
    42481 agttcggaaa gtgccggatc gcgttcctgg cagtctgcca gtttgcccgg caggcccgct
    42541 aagtcgagcg cacctttacg gcgggtcatt tcacgagcgc gacgcgccgc ttcacgggca
    42601 cgggcagcat cgataatttt gccaaccacg attttcgcgt cggttgggtt ttccagcagg
    42661 tattctgcca gcagttcgtt catctgctgt tctaccgccg atttcacctc agaagaaacc
    42721 agtttgtctt tggtctggga ggagaatttc gggtccggca cttttacgga aacgaccgca
    42781 atcaggcctt cacgcgcatc gtcaccggtg gcgctgactt tggctttttt gctgtagcct
    42841 tctttgtcca tgtaggcgtt cagggtacgg gtcatcgccg cacggaagcc tgccaggtga
    42901 gtaccgccgt cacgctgcgg aatgttgttg gtaaagcagt agatgttttc ctggaagcca
    42961 tcgttccact gcaacgccac ttcgacgcca ataccgtctt tttcggtgga gaagtagaag
    43021 atattcggat ggatcggcgt tttgttcttg ttcagatatt caacgaacgc cttgatgccg
    43081 ccttcatagt ggaagtggtc ttctttgccg tcacgcttgt cgcgcagacg aatggaaacg
    43141 ccggagttga ggaacgacaa ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg
    43201 gtcacattgg tgaaggtttc gaggctgggc cagaaacgca ccatggtgcc ggttttttca
    43261 gtctcgccgg taaccgccag cggagcctgc ggtacaccgt gttcgtagat ctgacggtga
    43321 attttaccct cgcgctggat aaccagctcc agtttttgcg acagggcgtt tactaccgaa
    43381 acaccaacgc cgtgcagacc gccggacact ttataggagt tatcgtcaaa tttaccgcct
    43441 gcgtgcagaa cggtcatgat cacttccgcc gccgatacgc cctcttccgg gtgaataccg
    43501 gtcggaatgc cgcgcccgtc atcctgtaca gagacagagt tatcggcgtg aatggtgacg
    43561 ataatttctt tacagtgacc cgcgagcgct tcgtcgatag cgttatctac cacctcgaat
    43621 accatgtggt gcagaccggt gccgtcatcc gtgtcgccga tatacatacc cgggcgctta
    43681 cgcaccgcat ccagcccttt caggactttg atactggagg agtcataaga attcgacatc
    43741 aacgtttctc gctcatttat acttgggtta atccgttatt ttaccctttt ccacggtaaa
    43801 catcttcgaa ttttcgtccg acatgtctat aacgtgttca gcactgatcg cgctgacaaa
    43861 gacctgtgat tgcgtcgctt ttaagcggct ggcaagcagc ccgcgacgct catcatcaag
    43921 ctcagaggca aaatcatcta tcaggtagag acaccgccgc ccgctttcac gggtgaggaa
    43981 ctctccttgc gccagacgta aggcgcacat caacagctta agctgcccac gcgataaggt
    44041 atcttccacc ggcgcaccgt cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc
    44101 ggtgtaggtt agctggcgat cgcgttcaaa attacgttcc agcacctcag catattctgt
    44161 ctctttctcc cagccgcgct ggaaagagaa agtcagagaa aactcaggga gaaattgctt
    44221 acaagtatcg gccatatcag ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat
    44281 ttgctccgcc agcgggatca gctctttatc ccacgggcgt agctgttcgt aacgtgtcac
    44341 ctggcgcagc gccgcattgc gctgcttgag caatcgcttg agattgctcc aggcggtgaa
    44401 aaatccgggt tcgttgtgaa agcatcccca gtcgaggaat gctcttctgt atttggggcc
    44461 gccgttgagt aaagtaaacc cttctggcgt tatcaactgc attggcatca ggtgcgccag
    44521 ttccgcgacc ttatgcccgt ctgtaccgtc gatgcggact ttgctgtcgc cctgtttgtc
    44581 tttggttaag ccaatcgctg tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa
    44641 cgcctcctgc tcatggcgaa tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg
    44701 gccgagcgta tagatggctt ccagcacgct ggttttgcca ctgccgttgg cacctaccag
    44761 aaagttaaag ccgggagata aggcgagatc cgcggtttca atgttgcgga aatcgcggat
    44821 caacaagcgg gtgagggaca ttacagtctc attggcatga caacataagc cgcgctctgg
    44881 ctggccgcat cttcaatctg cacgctggaa accgaatcgg tcagcatcat gcggacgttt
    44941 tcgcatttca gcgcgttcag aacatccagc acataactga cgttgaagcc gatttccatc
    45001 tccgcaccgc tataggtaac gtcgaggatc tcttccgctt cttcctgttc cgggttgttg
    45061 gcggtgattt tcagctggtt ttcgctgaca taaagacgta cgccgcggaa tttctcgtta
    45121 gagagaatcg ccgcgcgagc aaacgcctgc ttgagcagat cgcagccagc ttccagatgt
    45181 ttgtccgggt tcttcggcag aacgcggcga taatccggga agcgaccatc caccagtttg
    45241 gaggtgaaga taaagtcgcc aacgtgggcg cgaatgttgt tgctgccaat ctgtacgcgc
    45301 agcggattgt cgccgccgtc gagcatacgc atcagttcaa tcacgccttt acgcggtacg
    45361 atcaccgaat ggcttggcaa agattgacca attggcattg aacagaccgc cagacggtgg
    45421 ccgtcggttg ccacggtgcg cagttcttca ccttcggttt caaacagcat accatttaag
    45481 taatagcgaa cgtcctgatg cgccatagaa aactgggtcg cttcaatcag acgcttcatc
    45541 gttgcctgcg gcagggtaaa ttcgacttca ctctgccagt catcgaggtt cgggaaatcc
    45601 gccgctggca gggtagacag cgaaaaacgg ctacgcccgg agcgtaccag catccgttca
    45661 ccttccagct gcacggcaat ttccgcgcct tcaggcagac cacggcagat atcaaagaat
    45721 ttgcgcgccg gaacggtcgt cgctcctggc tcgtgtggct gaaccagcgc aacacgtgcc
    45781 accatttcca tctcgagatc agtaccggtc agcgacaacg taccgtcagc aacctgtaac
    45841 agcagattac cgagaatcgg tagcgtagga cgaccaccta acggaccgct cacctgttgt
    45901 agcggtttta ataaatgctc acgttctacg gtaaatttca taggtttacg atgacaatgt
    45961 tctgattaaa tttgaaaaat cttctttgat atcgtggctc tcttcacgca actgctcgat
    46021 cttacggcag gcatgaagca ccgtcgtgtg gtcacgacca ccaaacgcat cgccaatctc
    46081 cggcagactg tggttagtca gctctttcgc cagcgccatc gccatctggc gcggacgcgc
    46141 caccgagcgg gatcgacgct tggaaaggag atccgcgact ttgatcttgt agtactccgc
    46201 caccgtcttc tgaatattgt cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg
    46261 cagcgcctca cgcacgaagt cgatggtgat cgcccgtccg gtaaagttgg cattggcaat
    46321 gacgcggttc agcgcccctt ccagctcacg tacgttagat cgtagacgct tggcgataaa
    46381 gaacgccact tcgcccggca aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc
    46441 cacacgggtt tccagctctg gcggttcgat cgccacagtc agtccccaac cgaagcggga
    46501 tttcaaacga tcctcaacgc cgttgatctc tttcggatag cgatccgagg tgagaatgat
    46561 ctgttgatta ccttccagca gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc
    46621 tttattagca aaaaactgaa tatcgtcgat cagcagtgca tctacggaac ggtagtagcg
    46681 tttaaactct tcgatcgcgt tgttttgcag ggctttaacc atgtcctgaa caaagcgctc
    46741 ggagtgcata taaaccactt tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc
    46801 atgcagcagg tgagttttac ccagacccgt gccgccataa aggaacaacg ggttataggc
    46861 accgccaggg ttatccgcca cctggcgagc cgccgcgcgc gccagttggt tagatttacc
    46921 ttcaacgaag ttatcaaacg tgtgtttgac gtttacgtta gaacgatagg tcggttctgc
    46981 cggggccggg acgttatccc aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt
    47041 ttgcgccacc tgtgcagggg ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt
    47101 caccggtttg gtgccgactt caaaacgcag ctgtggggca tccgctccgc agaagctggt
    47161 tagcagtcca ttgatattat taaggtactt gtcccgtacc caatcgagga caaaacggtt
    47221 tggcgcgtac agggccagcg tgttatcgct cagttccgcc tgcaatgggc gtatccacat
    47281 actgaattct gtggctggta actcatcctg caatcgggca agacactgct gccaaagcga
    47341 aagtgacacg gcggactcca ctcgaacaaa agtcgataat gactaaggct gaaacattca
    47401 tgattgttga cgtacgtcga aaagaccctg cttgagggtg acgcacgaac cgctgtctgc
    47461 ggttatatgc ccgatcaaga tcctgcaaaa cgatcgggac cgcggatcat agcctaaact
    47521 gcgcaagaga tcttctgttt ctcacagatt ttcccgattt atccacagga ctttccagaa
    47581 ctcgctaagt gtaaacgatc ctgccgcgag gcgggcacga tttacgccgc atattggaaa
    47641 atttaatgac catagacaaa aattggctta atcgatctaa taaagatcca ggacgatcct
    47701 tgcgctttac ccatcagccc gtataatcct caacccggcg cgccatgctg gtttccactg
    47761 gtgtgaggtc gtacattttc cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag
    47821 agaattgact ccggagtgta caattattac aatccggcct ctttaatcac ccatggcttc
    47881 ggtgtccatc gtttcatttt tcggcggata tccaataaag ccattgaatt tattcaagtt
    47941 taggtagaaa tcgccatgaa acgcactttt caaccgtctg tactgaagcg caaccgttct
    48001 cacggcttcc gtgctcgtat ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt
    48061 gctaaaggcc gcgctcgtct gaccgtttct aagtaataaa gctaacccct gagtggttaa
    48121 gctcgcattt cccagggagt tacgcttgtt aactcccagt caattcacat tcgtcttcca
    48181 gcagccacaa cgggctggca cgccgcaaat taccattctc ggccgcctga attcgctggg
    48241 gcatccccgt atcggtctta cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa
    48301 tcggattaaa cgtctgacgc gtgaaagctt ccgtctgcgc caacatgaac tcccggctat
    48361 ggatttcgtg gtggtggcga aaaaaggggt tgccgacctc gataaccgtg ctctctcgga
    48421 agcgttggaa aaattatggc gccgccactg tcgcctggct cgcgggtcct gatagccctc
    48481 attcgggtct atcaactcct gattagtccg ctactcgggc cgcattgtcg tttcactcca
    48541 acctgttcaa gctacggaat tgaggcattg cgcaggtttg gagtgataaa aggcagttgg
    48601 ttgacggtga aacgcgtatt aaaatgccac cctttacacc ctggtggtga cgatcccgtc
    48661 ccgcccggac catttgatac cagagaacac taacgatgga ttcgcaacgc aatcttttag
    48721 tcatcgcttt gctgttcgtg tctttcatga tctggcaagc ctgggagcag gataaaaacc
    48781 cgcaacctca ggcccaacag accacgcaga caacgaccac cgcagcgggt agcgccgccg
    48841 accagggcgt accggccagt ggccagggga aactgatctc ggttaagacc gacgtgcttg
    48901 atctgaccat caacacccgt ggtggtgatg ttgagcaagc tctgctgcct gcttacccga
    48961 aagagctgaa ctctacccag ccgttccagc tgttggaaac ttcaccgcag tttatttatc
    49021 aggcacagag cggtctgacc ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc
    49081 cgctgtataa cgttgaaaaa gacgcttatg tgctggctga aggtcaaaac gaactgcagg
    49141 tgccgatgac gtataccgac gcggcaggca acacgtttac caaaacgttt gtcctgaaac
    49201 gtggtgatta cgctgtcaac gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg
    49261 aaatctcctc gtttggtcag ttgaagcaat ccatcactct gccaccgcat ctcgataccg
    49321 gaagcagcaa cttcgcactg cacaccttcc gtggcgcggc gtactccacg cctgacgaga
    49381 agtatgagaa atacaagttc gataccattg ccgataacga aaacctgaac atctcttcga
    49441 aaggtggttg ggtggcgatg ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg
    49501 acggtaccaa caacttctat accgctaatc tgggtaacgg catcgccgct atcggctata
    49561 aatctcagcc ggtactggtt cagcctggtc agactggcgc gatgaacagc accctgtggg
    49621 ttggcccgga aatccaggac aaaatggcag ctgttgctcc gcacctggat ctgaccgttg
    49681 attacggttg gttgtggttc atctctcagc cgctgttcaa actgctgaaa tggatccata
    49741 gctttgtggg taactggggc ttctccatta tcatcatcac ctttatcgtt cgtggcatca
    49801 tgtacccgct gaccaaagcg cagtacacct ccatggcgaa gatgcgtatg ttgcagccga
    49861 agattcaggc aatgcgtgag cgtctgggcg atgacaaaca gcgtatcagc caggaaatga
    49921 tggcgctgta caaagctgag aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc
    49981 agatgccaat cttcctggcg ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg
    50041 caccgtttgc actgtggatc cacgacctgt cggcacagga cccgtactac atcctgccga
    50101 tcctgatggg cgtaacgatg ttcttcattc agaagatgtc gccgaccaca gtgaccgacc
    50161 cgatgcagca gaagatcatg acctttatgc cggtcatctt caccgtgttc ttcctgtggt
    50221 tcccgtcagg tctggtgctg tactatatcg tcagcaacct ggtaaccatt attcagcagc
    50281 agctgattta ccgtggtctg gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct
    50341 gattcggtga gttttcgcta aaataagggc ggtcagttga ccgccttttt tcttttcgta
    50401 gggcggataa gcaccgcgca tccgccacac aaagcaacag gaacatcatg agcgataatg
    50461 acactatcgt agcccaggcc acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct
    50521 ccggcttcaa agcccgtgaa gttgccgaaa ccgtgctggg taaactgcct aagccgcgct
    50581 acgccgatta tcttccgttt aaagacgccg acggcagcgt gctcgatcag gggattgcgc
    50641 tatggttccc tggcccgaac tcgttcaccg gcgaagatgt gctggaactg caaggtcatg
    50701 gcggtccggt gatcctcgac ctgctgttaa aacgcattct gaccattccc ggcctgcgga
    50761 ttgctcgccc tggtgagttt tccgaacgcg cgtttcttaa cgataaactt gacttagccc
    50821 aggccgaggc gattgccgat cttatcgacg ccagttcgga acaggcggcc cgttcggcac
    50881 ttaactcgct gcaaggcgca ttctccgcac gggttaatca tctggtagaa gccctcaccc
    50941 acttgcgcat ttacgtcgaa gcggcaattg atttccccga tgaagagatc gatttcctct
    51001 ccgacggaaa aattgaagcc cagctcaatg acgttattgc cgatcttgat gcagtgcgtg
    51061 ctgaagcacg tcagggtagt ttgttgcgcg aagggatgaa agtggtgatt gccggacgtc
    51121 ctaacgccgg taaatcgagc ctgttaaacg cgctggcggg gcgtgaagcg gcaatcgtaa
    51181 ccgatatcgc cggaactacg cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc
    51241 cgctgcatat catcgatacc gccgggctac gtgaagccag tgacgaagta gaacgtattg
    51301 gtatcgagcg cgcgtggcag gaaattgaac aggccgaccg cgtgctgttt atggtcgatg
    51361 gcaccacaac agacgccgtg gatccggcag agatctggcc ggaatttatt gcccgtctgc
    51421 cagcgaaact gccgatcacc gtggtgcgca ataaagccga tatcaccggc gaaacgctgg
    51481 gaatgagtga agtgaacggt cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg
    51541 tggacgtgct gcgtaaccat ctcaaacaga gcatgggctt tgacaccaac atggaaggcg
    51601 gcttcctggc gcgtcgtcgc cacctacagg cgctggaaca ggcagcggaa catctacaac
    51661 agggcaaagc gcaactgttg ggagcctggg caggtgaact gctggcggaa gagttgcgtc
    51721 tggcacagca gaacttaagc gaaatcaccg gggaatttac ttcagacgac ctgctggggc
    51781 ggattttctc cagcttctgt attggtaagt aaccgcgctt acgaagccgt attctgactg
    51841 tcagatgcgg cttcgcttca ttgttagcac tcctgttatt cctcaaccct ttttttaaac
    51901 attaaaattc ttacgtaatt tataatctta aaaaaaagca tttaatattg ctccccgaac
    51961 gattgtgatt cgattcacat ttaaacaatt ttagaataga caaaattctt gagtattata
    52021 ctgtagcctc gtgtctttcg aggataagtg cattatgaat atcttacata tatgtgtgac
    52081 ctcaaaatgg ttcaatatcg acaataagat tgtcgatcac cgcccttaat ttacccttct
    52141 gtagccatca ccagagccaa accgattaga ttcaatgtga acgattagtt tgctatatct
    52201 taattttgcc ttttgcaaag gccatctctc gtttatttac ttattttagt aaatgatggt
    52261 gcttgtatat atacatggcg aattaatgga tattgcagat gtaatattca cagggatcac
    52321 tgtaattaaa ataaatgaag gattatgtaa tggaaaactt taaacatctc cctgaaccgt
    52381 tccgcattcg tgttattgag ccagtaaaac gtactactcg cgcttatcgt gaagaagcaa
    52441 ttattaaatc cggtatgaac ccgttcctgc tggatagcga agatgttttt atcgatttac
    52501 tgaccgacag cggcaccggg gcggtgacgc agagtatgca ggccgcgatg atgcgcggcg
    52561 acgaagccta cagcggcagc cgcagctact atgcgttagc cgagtcagtg aaaaatatct
    52621 ttggttatca atacactatt ccgactcacc agggccgtgg cgcagagcaa atctatattc
    52681 cggtactgat taaaaaacgc gagcaggaaa aaggcctgga tcgcagcaaa atggtggcat
    52741 tctctaacta tttctttgat accacgcagg gccatagcca gattaacggc tgtaccgtgc
    52801 gtaacgtcta tatcaaagaa gccttcgata cgggcgtgcg ttacgacttt aaaggcaact
    52861 ttgacctcga aggattagaa cgcggtattg aagaagttgg cccgaataac gtgccgtata
    52921 tcgttgcaac catcaccagt aactctgcag gtggtcagcc ggtttcactg gcaaacttaa
    52981 aagcgatgta cagcatcgcg aagaaatacg atattccggt ggtaatggac tccgcacgct
    53041 ttgctgaaaa cgcctatttc atcaagcagc gtgaagcaga atacaaagac tggaccatcg
    53101 agcagatcac ccgcgaaacc tacaaatatg ccgatatgct ggcgatgtcc gccaagaaag
    53161 atgcgatggt gccgatgggc ggcttgctgt gcatgaaaga cgacagcttc tttgatgtgt
    53221 acaccgagtg cagaaccctt tgcgtggtgc aggaaggctt cccgacatat ggcggcctgg
    53281 aaggcggcgc gatggagcgt ctggcggtag gtctgtatga cggcatgaat ctcgactggc
    53341 tggcttatcg tatcgcgcag gtacagtatc tggtcgatgg tctggaagag attggcgttg
    53401 tctgccagca ggcgggcggt cacgcggcat tcgttgatgc cggtaaactg ctgccgcata
    53461 tcccggcaga ccagttcccg gcacaggcgc tggcgtgcga gctgtataaa gtcgccggta
    53521 tccgtgcggt agaaattggc tctttcctgt taggccgcga tccgaaaacc ggtaaacaac
    53581 tgccatgccc ggctgaactg ctgcgtttaa ccattccgcg cgcaacatat actcaaacac
    53641 atatggactt cattattgaa gcctttaaac atgtgaaaga gaacgcggcg aatattaaag
    53701 gattaacctt tacctacgaa ccaaaagtat tgcgtcactt caccgcaaaa ctgaaagaag
    53761 tttaattaat actacagagt ggctataagg atgttagcca ctctcttacc ctacatcctc
    53821 aataacaaaa atagccttcc tctaaaggtg gcatcatgac tgatcaagct gaaaaaaagc
    53881 actctgcatt ttggggtgtt atggttatag caggtacagt aattggtgga ggtatgtttg
    53941 ctttacctgt tgatcttgcc ggtgcctggt ttttctgggg tgcctttatc cttatcattg
    54001 cctggttttc aatgcttcat tccgggttat tgttattaga agcaaattta aattatcctg
    54061 tcggctccag ttttaacacc atcaccaaag atttaatcgg taacacctgg aacattatca
    54121 gcggtattac cgttgccttc gttctctata tcctcactta tgcctatatc tctgctaatg
    54181 gtgcgatcat tagtgaaacg atatcaatga atttgggcta tcacgctaat ccacgtattg
    54241 tcgggatctg cacagccatt ttcgttgcca gcgtattgtg gataagttcg ttagccgcca
    54301 gtcgcattac ctcattgttc ctcgggctga agattatctc ctttgtgatc gtgtttggtt
    54361 ctttcttctt ccaggtcgat tactccatcc tgcgcgatgc caccagcacc actgcgggaa
    54421 cgtcttactt cccgtatatt tttatggctt tgccggtgtg tctggcgtca tttggtttcc
    54481 acggcaatat tcccagcctg attatttgct atggaaaacg caaagataag ttaatcaaaa
    54541 gcgtggtatt tggttcgctg ctggcgctgg tgatttatct cttctggctc tattgcacga
    54601 tggggaatat tccgcgcgaa agctttaagg cgataatctc ctcaggcggc aacgttgatt
    54661 cgctggtgaa atcgttcctc ggcaccaaac agcacggcat tatcgagttt tgcctgctgg
    54721 tgttctctaa cttagctgtt gccagttcgt tctttggtgt cacgctgggg ttgttcgatt
    54781 atctggcgga cctgtttaag attgataact cccacggcgg gcgtttcaaa accgtgctgt
    54841 taaccttcct gccacctgcg ttgttgtatc tgatcttccc gaacggcttt atttacggga
    54901 tcggcggtgc cgggctgtgc gccaccatct gggcggtcat tattcccgca gtgcttgcaa
    54961 tcaaagctcg caagaagttt cccaatcaga tgttcacggt ctggggcggc aatcttattc
    55021 cggcgattgt cattctcttt ggtataaccg taattttatg ctggttcggt aacgtcttta
    55081 acgtgttacc taaatttggc tgatcccttc aagaagccag ccatttcgct ggcttcttgc
    55141 ctgtcaggaa atctcttttg tccaaatggc aactcgcctg attctccttc accacgtatg
    55201 ctttgcgtca ccttactatc aggacgcttt agcccatgtc ccgcttttta atttgtagtt
    55261 ttgccctggt tttactttat cccgccggga ttgatatgta cctcgttggt ttaccgcgca
    55321 tcgccgccga tctcaatgcc agcgaagcgc agttgcatat tgcgttctcc gtatatctgg
    55381 cggggatggc agctgcgatg ttatttgccg gtaaagtggc cgatcgttca gggagaaagc
    55441 cggtcgccat acccggcgcg gcgctattta ttattgcctc ggtgttctgt tcactggctg
    55501 aaaccagcac gttatttctt gcaggccgat ttctacaggg gttgggcgca ggctgttgtt
    55561 acgtagtggc gttcgccatt ttgcgcgaca cgctggatga tcgacgtcgg gctaaagtgc
    55621 tgtcattact caacggtatt acctgcatca ttccggtgtt agcgccagtg ctcggacatc
    55681 tgattatgct taaattcccg tggcagagtc tgttctgggc gatggcaatg atgggcatcg
    55741 cggtactgat gttgtctttg tttattttaa aagaaacgcg cccagcgtcc cccgctgctt
    55801 cggacaaacc acgagaaaat agcgagtcgc tgcttaatcg gtttttcctc agccgtgttg
    55861 ttatcaccac cctcagcgtt tcggtgatcc tcactttcgt caatacatcg ccggtattgc
    55921 tgatggaaat catgggtttt gagcgcggag aatacgccac cattatggcg ttgaccgctg
    55981 gcgtcagcat gaccgtttca ttctccacgc catttgcgct gggaattttt aagccacgta
    56041 cgttgatgat cacctcgcag gtgttattcc ttgcagcggg gatcaccctt gccgtttcac
    56101 cttcccatgc ggtttctctg tttggtatca cgctgatttg cgccggtttc tcggtaggtt
    56161 ttggcgtagc gatgagtcag gcgttaggac cattttcatt acgcgcgggc gtagccagct
    56221 cgaccttagg tattgcgcag gtttgcggtt cgtcactgtg gatttggctg gcagcggtgg
    56281 ttggtatcgg cgcatggaat atgctgatcg ggattctgat tgcctgtagc atagtgagcc
    56341 tgttgctgat tatgttcgtc gcgcctggac gccccgttgc cgctcatgaa gaaatccatc
    56401 accacgcttg atctcaatct gctgctctgt ctgcaactgc tgatgcagga gcgcagcgtg
    56461 accaaagcgg cgaagcggat gaacgtgacc ccttcagcgg tgagtaagtc gctggcaaag
    56521 ttaagagcgt ggtttgacga cccgctcttt gtgaactcac cgctgggcct gtcgcccaca
    56581 ccgctaatgg tcagcatgga gcaaaatctg gcggaatgga tgcaaatgag caatctgctg
    56641 ctggataaac cgcaccacca gacaccgcgc ggcctgaagt ttgagttggc ggcggaatcg
    56701 ccgctgatga tgatcatgct taatgcgctg tcgaaacgga tctaccaacg ttacccgcag
    56761 gcgaccatca aactacgtaa ctgggattac gattccttag atgccattac tcgtggtgaa
    56821 gtggatattg gtttttccgg tcgcgaaagc catcctcgct cgcgggagct gttaagctcg
    56881 ctaccgttag ccattgatta tgaagtgctg tttagtgatg taccctgcgt ctggttacgc
    56941 aaagatcatc cggcactgca tgaaacgtgg aatctggaca cctttttacg ttatccgcat
    57001 atcagcattt gctgggaaca gagcgacacc tgggcgctgg acaatgtgtt acaggagctg
    57061 gggcgcgaac gcacgattgc tatgagcctg ccggaatttg agcagtcact gtttatggca
    57121 gcgcaacccg acaatctgct actggcgacc gcgccgcgct actgtcagta ctacaatcaa
    57181 ctccatcaac tgccgttggt tgctcttcct ctcccgtttg acgaaagcca gcaaaaaaag
    57241 ctggaagttc cttttaccct gctgtggcat aaacggaaca gccataatcc gaagatcgtc
    57301 tggttacggg aaaccattaa aaacctttac gcgtcgatgg cataaccgaa tcgtatgaaa
    57361 cgcgacccaa tttcacaata aaatgtaaaa aagttgtaat aagcttgtct gaatcgaact
    57421 tttagccgct ttagtctgtc catcattcca gtaaatgatt actcttatat tcataatgga
    57481 ccattaagca tggagcgaaa aatggcgact cactttgccc gagggatttt aacggaagga
    57541 catctgattt ctgttcgtct cccctcccag tgtcatcaag aagcccgaaa cattccccct
    57601 catcgtcaaa gccgttttct ggcgtccaga ggtttactcg cagaactgat gttcatgctg
    57661 tatggcattg gcgaattgcc ggaaatcgtc accctgccga aaggtaaacc ggttttcagt
    57721 gataaaaatt tgccttcgtt ttctatttcc tatgccggga atatggttgg cgtggcgtta
    57781 acaaccgaag gtgaatgtgg cctcgatatg gaactacagc gtgcgacgcg cgggtttcat
    57841 agcccacacg cgcccgataa ccacaccttt tccagcaatg aatcgctatg gatcagtaaa
    57901 caaaacgatc ctaacgaagc gcgggcgcag ctcatcacgc tgcgccgaag cgtgctaaaa
    57961 ctaaccggtg atgttttgaa tgacgatccg cgcgatctgc agctgctgcc cattgccgga
    58021 cgcctgaaat gtgctcatgt aaatcatgta gaagcgttat gcgacgcgga ggacgtgcta
    58081 gtgtggtccg tggcggtcac gcccgcgatt gaaaagctca gtgtctggga gttagatggc
    58141 aaacacagct ggaaaagcct gccggatatt cacagccgcg ccaacaatcc taccagccgg
    58201 atgatgcgtt ttgcccagct ctctaccgtg aagtcttttt cgccaaattg atagacaacc
    58261 acaggagtca ttatgtctga aaaattgcag gtggttacgt tactggggag cctgcgcaaa
    58321 ggctcattta atggcatggt tgcacgtacc ctgccgaaaa ttgctccggc gagcatggaa
    58381 gtcaatgcgt taccttccat tgccgacatt cccctgtatg acgctgacgt acagcaggaa
    58441 gaaggttttc cagcaacggt tgaagctctg gcggaacaga tccgtcaggc tgacggtgtg
    58501 gtgatcgtca cgccggaata taactactcg gtaccgggtg ggctgaaaaa tgccatcgac
    58561 tggctttccc gcctgccgga tcaaccgctg gcgggtaaac cggtgttgat tcagaccagc
    58621 tcaatgggcg tgattggcgg cgcgcgctgt cagtatcacc tgcgccagat tctggttttc
    58681 ctcgatgcaa tggtgatgaa caagccggaa tttatgggcg gcgtgattca gaacaaagtt
    58741 gatccgcaaa ccggagaagt gattgatcag ggtacgctgg accacctgac cgggcaattg
    58801 accgcatttg gtgagtttat tcagcgagtt aagatctaaa taaaaaaccc gccagtaatc
    58861 atgcatggcg ggtttttaat gcgctatcga ttttaatgag cgtcgataaa tacaatcttc
    58921 aggataaaca gcagcgcaac gatgattacg cacgggctaa ggtcacgcag acgcccggta
    58981 ccaatcttca tcacgcagta ggagataaag cccagcgcaa taccttcggt aatcgaaaag
    59041 ctgaacggca tcatcacggc ggtaataaac gccggaacag attcagtaag atcctgccag
    59101 ttcacgcgag caagacttga ggtcatcaac acgccaacgt agatcaacgc gccagctgca
    59161 gcgtagcctg gcaccattcc cgccagcggc gacagaaaga taaccagcag gaacagcaga
    59221 ccaacaacca ctgccgtcag accggtacga ccgccaaccg atacgccgga agaggactca
    59281 atataagccg taacggaaga agtaccgata aacgaaccgg tcacggaaga gatactgtcg
    59341 acatacagcg cctgcttcat gcgcgggaat ttccccttct catccgccag acctgcttta
    59401 tcggtcacgc caatcagcgt accggaggag tcaaacaagt tgaccaacat gaaagagaaa
    59461 atcacccctg ccagcccgag gttaaacgac ccggctaaat ctacatgacc cacaactgtc
    59521 attacgctcg gcggcgcaga aacgatgcca ttgtagtgca catcacccag catccagccc
    59581 agcagcgtcg tcaccacgat agaaaccagc accgctgcgt gaatgttgcg cgaggccaga
    59641 atagcaatga tgaagaagcc gaggataccc agaagtacgc tgtgagaagt cagattaccg
    59701 atgctcacca gcgtttccga gttagcgaca atcacacctg cgtttttcag ccccatcatg
    59761 ccaatgaaca gaccgatacc gctggtaata cccacacgca gactcaccgg aatgttggct
    59821 atcatccagt agcgaacgcg gaaaatcgtc agtaacagca gacctatcgc gccccagaag
    59881 attgcgccca tcccgacctg ccacggcaag cccatcgcct gtacaacgac aaaagcgaag
    59941 aacgcattca ggcccatagc gggtgccagt gcaactggca ggttagcaaa cagtcccatc
    60001 ataatactgc cgaatgcagc gatcagacag gtagtgacga agacggcgct ggtatccatg
    60061 ccagcaacgc caagaatttg cgggttaaca aaaacgatgt aaaccatcgt caggaaggtg
    60121 gtaaaaccgg cgatcacttc ggtccgtgcc gtcgtgccat gttcgcgcag tttaaacacg
    60181 cgttccagca tcccctgacc agaagtctgg gtggtatgtt gatgactcat tatctatttc
    60241 cgaacaagga gggaaaatcc gtcgctatcg tataccaaaa tgcgacaata ggcgcgtttg
    60301 tgagagactt ttttattgga tttacttata cggcaacgat tgcgttgcgc aaatcggctc
    60361 tacgaaaacg ttaaactgat taaaaaggaa aggcatgtcc cggatagaag cggtattttt
    60421 cgactgcgac ggtacgctgg tcgacagtga agtcatttgc tctcgcgcat atgtaacgat
    60481 gtttcaggaa tttggtatta cgctcgatcc tgaagaggta ttcaaacgtt tcaaaggtgt
    60541 aaaactgtac gaaattatcg atattgtttc ccttgaacat ggtgttacgt tagcgaaaac
    60601 agaagctgaa cacgtttacc gtgcagaagt cgctcggctg ttcgattcag aactggaagc
    60661 catcgaaggg gctggagcgc tcctgtcagc gatcactgcg ccaatgtgtg tggtatctaa
    60721 cggcccaaat aacaaaatgc agcattctat gggcaagctg aatatgttgc actacttccc
    60781 ggataaactg ttcagcggct acgatattca gcgctggaag ccagacccgg cgttaatgtt
    60841 ccatgcggca aaagcgatga atgtaaatgt agaaaactgc attctggttg atgactcagt
    60901 tgccggtgcg caatctggta tcgacgcagg tatggaagtg ttctacttct gcgccgaccc
    60961 gcacaataag ccgatcgttc acccgaaagt caccaccttt acccatcttt cgcagttacc
    61021 ggaactgtgg aaagcgcgtg gttgggatat tacggcatag ttcttcacac tcccttcact
    61081 taccccgctt aaattggcgc tcaaaggtaa gttaagggtg tttgatatgt ctgtttcacg
    61141 tcgggtaata catcacggac tttattttgc ggttttagga ccgttaattg gtgttctgtt
    61201 tcttgtcctc tacatattct tcgcaaaaga accgctggtt cttttggtaa taatacaggt
    61261 acttccattg tttctcttat tgtcgattac tacgggagct attcctgcat tgttaaccgg
    61321 cgtgatggtc gcctgtctgc cggaaaagat cgggtcacag aaaaattatc gttgtctggc
    61381 tggtggcata ggtggcgtcg ttatcaccga gatctattgt gcagttattg tacatattaa
    61441 gggcatggct tcctcggagt tgtttgaaaa cattctttct ggtgacagtc tcgttgtccg
    61501 catcattcct gcattgctgg caggtgtggt gatgagcaga atcattaccc gtctgcccgg
    61561 attggatatt tcatgtcctg aaacagactc tttaagttaa gcgggatact ttatctttgg
    61621 gctactcaaa agcagacagg atgttactat gactcaaaat atcaggccgt taccccaatt
    61681 caaatatcat cccaagccac tggaaacagg cgcatttgaa caggataaaa ccgtagagtg
    61741 cgattgctgt gaacaacaga cgtcagttta ttactcgggt cccttttatt gcgttgatga
    61801 agttgaacat ctctgtccgt ggtgtattgc ggacggttct gctgctgaaa agtttgcagg
    61861 tagttttcag gatgatgcca gcatagaagg tgttgaattt gagtatgatg aagaggacga
    61921 atttgccggt attaagaaca catatcctga tgaaatgctg aaagagttgg ttgaacgcac
    61981 gccaggttat catggatggc aacaggaatt ctggcttgcg cattgtggcg atttctgtgc
    62041 ttttatcggc tatgtgggct ggaatgatat aaaagatcgc ctcgatgaat ttgccaacct
    62101 tgaagaagat tgtgagaatt tcggtattag aaattctgat ctagctaaat gcctgcaaaa
    62161 gggtggtgat tgtcagggtt atctcttccg ctgtctccac tgcggcaagc tgagactgtg
    62221 gggtgatttt tcgtagttat ttaaataatg agaacaggcc ggagcgtaat tcatacatcc
    62281 ggccttattt cttcagctta accgagcgct aattctgcgg cagcggcttt atcagcgatt
    62341 accatcagcg aaggatgcag ctgcaacacg gaagctggaa catcttcggt aaccggacct
    62401 tgcagcacat ttttcagtgc ctgcgcttta cctgcgccac tgacaattat cagcaagttt
    62461 ttcgccgcca tgatgctttt cggccccatc gtcacgtagc tgtcaggcac taacgaaaag
    62521 tcgccgccca actcaccatg cgccacaata taaaccatct ctccctggat cgggaactcc
    62581 acggtttgct catggaaatg ggtcgtattc ggcaaattgc cacaaaaatg accatctgca
    62641 cctaatccca gcaccaccaa atccaggccg ccttcacgcg ccagtttctg atcatgctcg
    62701 cggtagttat caatagtgag cttctggata ttctcttctt tgatccccgc aggggtgaaa
    62761 aacagattac gcagattggt aatcgttaca ccttctccct ctttgccgcg aaagggaatt
    62821 tcatcgaaat tatagaaata gcagttatcg taccagggct tacctttaac cagggtggtg
    62881 aggtattcat acatgccttt gggcgtgcta ccggcggtaa ttgccaggtt aacacgacgc
    62941 gtcttcgaca tataaccaag cagatggtgt gccgcgacac ggctcatttc ctggtaatct
    63001 tcggtaatga ttaatttcat cttagtgcct tattttattt aaataatttc tgtacgaagg
    63061 ctgcataggc aggccgccag acatccattt catggttcag gccgggatat tcctggtaat
    63121 caaacttaat ttttttctgc tcaagctcag ttttcagccc ggtgatatcc ttgccggtta
    63181 cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta atagctgccg
    63241 gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta acaccactga
    63301 atgtggccag ccagccaaag ctttccagat gattcattcc ggaaaccagc gcgtggtacc
    63361 cgccttgtga aagccctgcc agcgcgcgac catcggcatc tttacggaca ttaaaacgct
    63421 tgctaatcag cgggataata tcgttcatca gttcgcgatc tgccgcttta gcattcagcg
    63481 gataaaagac tttacgtctt tcctgaggca cgaaatcttc gggaataatg cccttcgcat
    63541 cggtttcggt atccgggatc accaccagca tcggtttaat tttcccttca gccagcaggt
    63601 tatccatgat ttgcgggata cgcccctgat cgatagcgga acgtccggta tcaccaaaac
    63661 cgtgatagaa atagagcact ggcaaaggct cgcccatgcc ggtgtatcct ggcggggtcc
    63721 agacatacat ctgacgttca gattgcaatg cgttggagtg gtaagttatg gcgatcaaat
    63781 caccatgcgc aacagaacgc gtatccagat aactgcctgg caccagaatc atactggagt
    63841 taacctggcg ctgaggctta gtcattgcgg tgcctgtatc aatgctgcgt acaccatcaa
    63901 cattgaaaaa atactcgtac aggttgcctt tcaggatagg tgtgcgccac gaccagactc
    63961 ctgcttcgtc tttggtcatc gggtgaatat tgtccggaac cggaacaccc accactacag
    64021 agacattttt tgccccaggg gcaaagtagc gaaaggtcac gctgttatcg gcattgacct
    64081 gagtcacata ctgtttcacc gggatagtgg gtgccgggct ggctggcata tccgctgcta
    64141 tggcccactg agcagaaata ccgctggcaa tagccagcgt taaagcagca atttttatat
    64201 tcatcgtaat attcctttca ttaagttcca ttaccaccag atttcagcct gggcaccgac
    64261 agcaaactgg tcgtctttat tgtcttcgaa ggtgaagcca tccagttcgt tttccagggc
    64321 tttgatatac gtggcgtaga aacgaatttc cggacgcgag gtcaacatac tggtattgac
    64381 tttaaaggta tggaagagcg tggttttata accagactca ttaaatttat tactattcgc
    64441 atctttgttt tgctgggtga aatagcccag ttcaacacct gtctggttat attggtccca
    64501 aatataggcc gggcgaacaa ccgcacgaat agattcgaaa tcagagtgtg cgcctgtttc
    64561 gtagctataa atatcgttac cgaaggagta aacaatcgcg ttagccacaa taaaatgatc
    64621 gccgatatag gcttcgccct gcgaggtcag acgaactgct gttccgccgg tgtgatcacc
    64681 ataataacga ccattaaagg tggtaaatgg acttgcgcca gcataacggc caaagttact
    64741 ggcgatagag ttattcgcaa ccaggaagga gaattcgttg aagccacctt tatcaaattt
    64801 ctgcgttaaa gatgtgccga acatccaggt atctttccag tcataatacc cgttattatc
    64861 ctgattatct ttttccgatg cgctttcgtt tgccgtgaca taacgaccac tcaccattaa
    64921 ggtcgcttta tcccataacg ggatatcttt atagcgtaaa tcaatagtat tggtattaat
    64981 ctgctgtttg ttttgcaggc tgcgatcgta atcatcgaca tcttcgcgaa ccagcgcgat
    65041 atcaatttta cccggaccga ctttccagtt ttccagacct acacccgctg cggcatcagt
    65101 acgctgcgtt ttccagtcca gcatctggat ttcaattttc ggcgcaccgt gtttacccac
    65161 ccagaaatca gcctctggcg caaagggcag gaaacctttg gtggtaacgt acatatcgga
    65221 gaactgcata aagttctcgc caccggcgtt atcgccaaac cagccggtag agtactgctg
    65281 accaacgtta ccatccatca tcacaacggc atcaacccgt ttgccgtttt cgttgtagac
    65341 acgttgtttt aactgcaaat caaaccagcc ggagtattcg ttaccaaagc ggcccagaga
    65401 accaatcgcc catgatttag gtgaaccatg agaggcggtc ccccaaccag aacggtaata
    65461 gccgttgtaa ctaaagccaa tttcatcttt cacaaactta ctgaaatctt tcagagtcat
    65521 ggaagaataa atcgagcctg tctcactggc atttttctct tcatttttga ccagtacagc
    65581 atcaggtttt gcggcactac tggtcgggaa cggattggcg gcataccctt gatcattcgt
    65641 acttacgcta cgattcaccg tcgctggcgt atattttttc ttctcttcat ctttatactt
    65701 tttgagttcg ctttgcgttt ctcttaacgc tttttctaat aactcaaggc gttgttccac
    65761 cgttaatgat tcagccaatg attgtgcaga aaaggctaac ggtgccatta ataagacggc
    65821 agaggtaata agatttcgtc taaacataat atccctttat ggtgcaaaga aagaattaac
    65881 gtatcgcatc cagactgttc tgaatgcgac gataattaag gtgctttatt ggttattttt
    65941 ttaatgacag cccccgcgtt ttgatcactt ctgcatacca tccgaagctt ttcttacgcg
    66001 ttcttgtgag gcttccttcg ccattatcat cacgatccac ataaataaag ccgtagcgct
    66061 tagacatttg tgaatgagat gcactgacta aatcaattgg cccccaactg gtgtacccca
    66121 taatatccac accatcggca atcgcttcat ttacctgtac caggtgatcg tttaaatagg
    66181 caattcgata atcgtcctgt atcgaaccat ccgcttcaac gctgtctttt gcgcctaatc
    66241 cgttctcgac aataaataac ggtttttgat aacgatccca aagcgtattt aacagaaccc
    66301 gtaatccaac cggatcaatt tgccaccccc actctgaact tttcagatgc ggattgggga
    66361 tcatattcag tatgttgccc tgcgcatttt tattaatgct ttcgtcgtgg gaaacacaac
    66421 cagtcatgta ataactaaaa gagatgaaat cgacggtatg ttttaaatct tctgcgtcac
    66481 tttcagtcat ctcaatggtg atattgtggt cgcggaagaa acgctgcata tagccgggat
    66541 actggccacg cgcctgaaca tcaccaaaga acatccagcg ccggttctct tccatggcct
    66601 gcaacatatc ctgtggctgg caggtgaggg ggtaaaccag cccaccgaga agcatattgc
    66661 cgattttcgc ttcggggagc aggctatgac aggctttaac tgcccgcgca ctggcaacca
    66721 gttgatggtg gatagcctga taaacttccg cctcgccact ctcttctgcc agccccacgc
    66781 ccgtgaatgg cgcgtgtaac gacatgttga tttcattaaa cgtcagccat aacgccactt
    66841 tatgttggta gcgagtaaag accgtgcggg cgtaatgctc gaagtgatcg atgaccgctc
    66901 gattagccca accgccgtag tttttcacca gcccatatgg catttcgtaa tgggataacg
    66961 ttaccagcgg cttgatcccc gcctgcgcca tttcatcaaa cagccgatcg taaaacgcta
    67021 accccgcttc attcggttcg acttcgtcgc cctgagggaa aattcgcgcc caggcaatgg
    67081 aaatacgcag acaggtgaag cccatctcgg caaataacgc gatatcttcc gggtaacggt
    67141 gataaaaatc gatggcgaca tctttgatat tctctttccc caggatgcgc ggttccattt
    67201 ttcccattac gccatgaggc tgtaaatctg aggtcgagat ccctttgcca tcttcctgcc
    67261 aggcaccttc cacctgattg gcagctgttg cgccacccca aagaaatgtt tctggaaatg
    67321 ctttcataat taactccttt tatcgttagc gaatgatgga taacagcggt tcacctgcgc
    67381 ttatctgcgc cgtgccgtgg ggtaatacgt ccgtaaaatc atcgctatta ctgattaata
    67441 ccggcgtcgt cagatcaaat ccggcctcgc gaatagcagg gatatcaaaa gaaatcagcc
    67501 gatcgcctgt attgaccttg tcacccacgt tgacgtgagc ggaaaagaat ttgccgtcca
    67561 gttttacggt gtcgataccg acatgaatca ggatctccac accatcatct gactcaatgc
    67621 caatggcgtg taatgtggcg aacaacgaag caattcgacc cgcaaccgga gaacgcactt
    67681 caccaaccga gggcagaatg gcgatacctt tacccaacag gccactggca aacgtggtat
    67741 cagcgacgtg aatgagcggc acaatctctc ccgtcatcgg tgaacagata ccgccctgct
    67801 caggtggtgt aataacctct ggtgttttct cttgcggggc accctgcgct ggctgacgtt
    67861 tagcggtgat gaaatgaagc atcaccgtac cgacaaatgc gcaaccgatg gcaatgacac
    67921 cgccaataac gctggcccag acggtgaaat caattcccgt tgacgggatg gtttgcatga
    67981 aggtgaaaat acttggcaaa ccaaaggagt agactttcgt ttgcgcgtag ccaatgatgg
    68041 tggcccccaa agccccactg atacaggcga taacaaaggg gtacttacgc ggcaggttga
    68101 cgccatatac cgctggttcg gtgataccaa acagactcgt caacgccgct gatcccgcca
    68161 ccactttttt ctgcgcatcg cgttcgcaga ggaagacgcc gagcgccgcc ccgacctgcg
    68221 ccataatggc gggcattaac agcgggatca tggtgtcgta gcccagcacg gtgaagttat
    68281 tgatacacag cggcaccagg ccccagtgca gtccgaacat gacgaagatt tgccagaagc
    68341 cgcccattac cgcgcccgca aatgcaggaa ccgcctgata aagccagaga taaccggcgg
    68401 caatcagttc gcttatccag gttgatagcg gccccaccag cagaaaggtg acgggtgtga
    68461 taaccatcag acatagcaat ggtgtgaaga aatttttgat tgccgacggt aaccacgcat
    68521 taagtcggcg ttccagaatg ctgcacaacc aggcagaaaa aataatggga ataaccgatg
    68581 acgagtaatt caacaatgtg accggaatac ccaggaaatc cagccccagc gcatccgctt
    68641 tttgcccgtt ctcgaaagca gtcagaatta atggatgcac taacgctcca ccaatcacca
    68701 tggcagtaaa tggattaccg ccgaagcgtt tccccgcggt gtatcccagg attatcggga
    68761 agaaccaaaa caaggcatca ctggcgctga ataaaattaa ataagtacca ctttgttcgg
    68821 tcgtccactg aaaagtgagc gccagagcca gcataccttt caagatcccg gttgccgcca
    68881 tcaaaccgat cagaggcgta aaaatacctg aaataacata aacaaagcgg tttagcagat
    68941 tacctttatc atcattttcc ggtgcctgtt gcgctttttc gtcaaggcct gccacactgt
    69001 taaccgccag gaagacatcg gccacatggt tacctatgac cacctgaaac tggccaccgc
    69061 tttccaccac cataataata ccgggggtct ttttcagtac ctctgcttgc gctttgcttt
    69121 catcctttaa tttaaaacgt aatcgcgttg cgcaatgcat cagactcaca atgttatctg
    69181 cgcccccgac tcctgcgact atttttctgg ctaactccgt cataacttgc cctctaccgc
    69241 tttgcggcaa aactccaaaa aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt
    69301 tttttcaggt tttgcccgct tagtgcggta acaatccttt actcagtaat aatatttcag
    69361 tgttctttgc gcacgcgctc tatatttatg gctaaaaaca taatctctgc gggtgaaatt
    69421 ttacgttgat actgcaaacc aataaaaatg gcgatccgtt ccgcacattg ccatgcttgc
    69481 gggtaatttt gttttactgc ttgttgtaat gattcatcac tatcgttaat tgaagcatgt
    69541 tcaagaatac gccaggataa aaacttcaga tgtgtaacca gtcgctgata actcaagctt
    69601 tcttcctggt aattaaggct gaactgaaat tttattaatt gcagcatttc gcgcattaac
    69661 tgcgtgacac ctgcaacatc ctccatattt ccgctcattt gggcactgac cagatgcatg
    69721 gcaataaagc ccacttcatc tttcggtaac tgcacgccca accgtttatc aataatggtt
    69781 aacgcttctt cccctagctg gaactctttc gggtaaagcc gctggatatc ccacagcaac
    69841 gggttgggca gcaacacgtt ttgctgaaag cgtttaatcg caaactggca atggtcagtt
    69901 agcgagatat aaatactgtc ctgtaatttt cccaagcgct cctgcgctaa agagataata
    69961 cgatcacagg ttgccatcac ctcaagagga atatgactta agagttcgct taatcgcccg
    70021 ttcagttcat gactgctcaa ggcatactct ttttctattc cacttgagtt aattctttcg
    70081 ccagcgcgtt tttgaaagcc aattccgcgc cccatgacga ctttttcccg ctgttgatca
    70141 tcaataacca ccacaacatt attgttgaga attttggtga tttgcatgtt catagcaagg
    70201 acctttttta taaacaaaaa aaaccgactt caccagtatt ctctggttat gtcaggtttt
    70261 gcctgcgaat gcagtaacaa tccagtcatt tattaatggt ttttataacg aacatccagg
    70321 ttcggaaatt aatttaattg cgtgcagaga aaatagcaat gcgctattga ta
//
LOCUS       DAOFQD010000008        69017 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.008, whole genome shotgun sequence.
ACCESSION   DAOFQD010000008 DAOFQD010000000
VERSION     DAOFQD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.008"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(194..1786)
                     /gene="lsrK"
                     /locus_tag="QBF46_000528"
     CDS             complement(194..1786)
                     /gene="lsrK"
                     /locus_tag="QBF46_000528"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDQ6672759.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1865..2818)
                     /gene="lsrR"
                     /locus_tag="QBF46_000529"
     CDS             complement(1865..2818)
                     /gene="lsrR"
                     /locus_tag="QBF46_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDQ6672760.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3067..4602
                     /gene="lsrA"
                     /locus_tag="QBF46_000530"
     CDS             3067..4602
                     /gene="lsrA"
                     /locus_tag="QBF46_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDQ6672761.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIEGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSRDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTERDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4596..5624
                     /gene="lsrC"
                     /locus_tag="QBF46_000531"
     CDS             4596..5624
                     /gene="lsrC"
                     /locus_tag="QBF46_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDQ6672762.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5624..6616
                     /gene="lsrD"
                     /locus_tag="QBF46_000532"
     CDS             5624..6616
                     /gene="lsrD"
                     /locus_tag="QBF46_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDQ6672763.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAMLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6628..7650
                     /gene="lsrB"
                     /locus_tag="QBF46_000533"
     CDS             6628..7650
                     /gene="lsrB"
                     /locus_tag="QBF46_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDQ6672764.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7677..8552
                     /gene="lsrF"
                     /locus_tag="QBF46_000534"
     CDS             7677..8552
                     /gene="lsrF"
                     /locus_tag="QBF46_000534"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDQ6672765.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAMRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8576..8866
                     /gene="lsrG"
                     /locus_tag="QBF46_000535"
     CDS             8576..8866
                     /gene="lsrG"
                     /locus_tag="QBF46_000535"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDQ6672766.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8923..9681
                     /gene="tam"
                     /locus_tag="QBF46_000536"
     CDS             8923..9681
                     /gene="tam"
                     /locus_tag="QBF46_000536"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDQ6672767.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9685..10599)
                     /locus_tag="QBF46_000537"
     CDS             complement(9685..10599)
                     /locus_tag="QBF46_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HDQ6672768.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10806..12257)
                     /gene="uxaB"
                     /locus_tag="QBF46_000538"
     CDS             complement(10806..12257)
                     /gene="uxaB"
                     /locus_tag="QBF46_000538"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDQ6672769.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12484..13902)
                     /locus_tag="QBF46_000539"
     CDS             complement(12484..13902)
                     /locus_tag="QBF46_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDQ6672770.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSLSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14041..14400)
                     /locus_tag="QBF46_000540"
     CDS             complement(14041..14400)
                     /locus_tag="QBF46_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDQ6672771.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14400..15326)
                     /gene="glsB"
                     /locus_tag="QBF46_000541"
     CDS             complement(14400..15326)
                     /gene="glsB"
                     /locus_tag="QBF46_000541"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDQ6672772.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15390..16778)
                     /gene="sad"
                     /locus_tag="QBF46_000542"
     CDS             complement(15390..16778)
                     /gene="sad"
                     /locus_tag="QBF46_000542"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDQ6672773.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16879..17724
                     /locus_tag="QBF46_000543"
     CDS             16879..17724
                     /locus_tag="QBF46_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6672774.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLDGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGYAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVIAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLRGRLRKRKIKHAKPVA
                     AAVAA"
     gene            complement(17693..20659)
                     /locus_tag="QBF46_000544"
     CDS             complement(17693..20659)
                     /locus_tag="QBF46_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element ISPa38 family transposase"
                     /protein_id="HDQ6672775.1"
                     /translation="MPRRSILSAAERESLLALPDTKDELIRHYTFSESDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGADEPPFPPLLRLVANQLKVGIESWDEYGQREQTRRE
                     HLVELQTVFGFQPFTIGHYRQAVQLLTELAMQTDKGIVLARALIEHLRRQSVIVPALN
                     AVERASAEAITRANRRLYDALAEPLTDVHRRRLDDLLKRRDNGKTTWLAWLRQSPVKP
                     NSRHMLEHIERLKAWQALDLPSGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALAIEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLFGRIG
                     QALIEAKQAGRDPFAAIEAVMSWDAFAESVTEAQRLAQPEDFDFLHRIGESYATLRRY
                     APEFLDVLKLRAAPAAKDVLDAIEVLRSMNSDNARKVPTDAPTEFIKPRWQKLVMTDT
                     GIDRRYYELCALSELKNALRSGDIWVQGSRQFKDFEDYLVPPAKFASLKQASELPLAV
                     ATDCDQYLHDRLTLLETQLATVNRMALANELPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMILPHVKITELLLEVDEWTGFTRHFAHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHIRDETYGAALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDATYEALKP
                     MIGGTLNIKHVRAHWDEILRMATSIKQGTATASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAANALRGHGQAVDDGLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            20818..21108
                     /locus_tag="QBF46_000545"
     CDS             20818..21108
                     /locus_tag="QBF46_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDQ6672776.1"
                     /translation="MRPSVVLDMKRSAVREAVGRFRAANPRVFGSVLHGTDRDGSDLD
                     LLVDALPGATLLDLGDLEEELKSLLGVDVDLLTPGDLPPKFRAKVLAEAQPI"
     gene            21105..21506
                     /locus_tag="QBF46_000546"
     CDS             21105..21506
                     /locus_tag="QBF46_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HDQ6672777.1"
                     /translation="MSENRLPDYLDHIQQAATDARSFVEGMAKDDFLADKRTQQAVIM
                     SLIVIGEAATKVMDGYVEFTQAHADVPWRSMRNMRNRMAHGYFDINLDVVWETVQEWL
                     PALLQQLPAVRQDADDEDRNDNCERGISDDC"
     gene            21496..21852
                     /locus_tag="QBF46_000547"
     CDS             21496..21852
                     /locus_tag="QBF46_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDQ6672778.1"
                     /translation="MTVESRIFSVAEYVQPSEGEPIRSVVLETRDSIIVVWHVHPGQE
                     IAAHIHPHGQDTWTVLSGMADYFQGNGIVRALREGEIAVARPGQVHGARNTGTEPFVL
                     VSVVASANAGFVLAER"
     gene            22107..22433
                     /locus_tag="QBF46_000548"
     CDS             22107..22433
                     /locus_tag="QBF46_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672779.1"
                     /translation="MSRSRRKTPIVGHTTCGSEREDKKLWHQRWRTRERTALTSASPE
                     ALSAHLPLLENQASSVWSMGKDGRSYWPVKRQAATADRIANHKGRNPQERASLKKRLL
                     RKWMSK"
     gene            22430..22930
                     /locus_tag="QBF46_000549"
     CDS             22430..22930
                     /locus_tag="QBF46_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6672780.1"
                     /translation="MKLSFHQHIALFWMIGAPGVFAPVIENAKRPDAGAVMAWGVAIV
                     AVMILFTPLLLRCPPFRRWYGRTDALSERQRQALAERGLRRYYQTAFDDGYVPRVMPY
                     VWRIIWTVGGLMAVTAVLPTNTGRPAFDALVVFSTWYPIGVMLLVFASRPLGRLIRQR
                     AQERRK"
     gene            22927..23298
                     /locus_tag="QBF46_000550"
     CDS             22927..23298
                     /locus_tag="QBF46_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672781.1"
                     /translation="MSTSTIEALASAWARIAEEAEFPADYEGTATPQAHRASEAIQEQ
                     IRERIVATNDMRLFSLLHLLGQASLRMEQALWPEDYERMTREVEEALRQATDANARSY
                     THEEVMQAMQERIDRARDKPC"
     gene            23292..23849
                     /locus_tag="QBF46_000551"
     CDS             23292..23849
                     /locus_tag="QBF46_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6672782.1"
                     /translation="MLIGYARVSTQDQNLELQREALSKAGCKKVFEDKVSGTRADRPG
                     LAKTLEMLREGDTLVVWKLDRLGRSVKQLVDLVGDLHKHGVQFRSLTDSIDTGTPSGR
                     FFFHVMASLAEMERELTVERTRAGLEVAKQLGRKGGRKPKMTDSKIESAKKLLASGVP
                     PKDVAKNLGVSIPTLYRWVPASTHA"
     gene            23884..23988
                     /locus_tag="QBF46_000552"
                     /pseudo
     CDS             23884..23988
                     /locus_tag="QBF46_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416043.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            24066..25181
                     /gene="yneK"
                     /locus_tag="QBF46_000553"
     CDS             24066..25181
                     /gene="yneK"
                     /locus_tag="QBF46_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDQ6672783.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINLGVPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWSNSE
                     VHRWFVNKDPNLITAILDITDGELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQE
                     SPHFQDFFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATVKTPDLPERH"
     gene            25331..26521
                     /gene="ydeA"
                     /locus_tag="QBF46_000554"
     CDS             25331..26521
                     /gene="ydeA"
                     /locus_tag="QBF46_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDQ6672784.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(26546..27211)
                     /gene="marC"
                     /locus_tag="QBF46_000555"
     CDS             complement(26546..27211)
                     /gene="marC"
                     /locus_tag="QBF46_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDQ6672785.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(27332..27616)
                     /locus_tag="QBF46_000556"
     CDS             complement(27332..27616)
                     /locus_tag="QBF46_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6672786.1"
                     /translation="MAVKGIKFSEVKAKALANPDVKKAYDEATQEEELRAVLIEMKKR
                     AGLTSTEIAARMGVSQPAVSRLERNVSSASLTTLQRYAAACGMHIKLSLQ"
     gene            complement(27606..27926)
                     /locus_tag="QBF46_000557"
     CDS             complement(27606..27926)
                     /locus_tag="QBF46_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDQ6672787.1"
                     /translation="MYELLFHPEAESEIYDLEPIMQAKVLKGLEKLEAKGPELRYPDT
                     GIIQDGLFELRVGRKDISRTFFAYAKGRKIYILRTFIKKTPKTPKAEIALATSRWEEL
                     KDGC"
     gene            28145..28579
                     /gene="marR"
                     /locus_tag="QBF46_000558"
     CDS             28145..28579
                     /gene="marR"
                     /locus_tag="QBF46_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDQ6672788.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            28599..28982
                     /gene="marA"
                     /locus_tag="QBF46_000559"
     CDS             28599..28982
                     /gene="marA"
                     /locus_tag="QBF46_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDQ6672789.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            29014..29232
                     /gene="marB"
                     /locus_tag="QBF46_000560"
     CDS             29014..29232
                     /gene="marB"
                     /locus_tag="QBF46_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDQ6672790.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(29263..30162)
                     /gene="eamA"
                     /locus_tag="QBF46_000561"
     CDS             complement(29263..30162)
                     /gene="eamA"
                     /locus_tag="QBF46_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDQ6672791.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            30357..31544
                     /gene="ydeE"
                     /locus_tag="QBF46_000562"
     CDS             30357..31544
                     /gene="ydeE"
                     /locus_tag="QBF46_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDQ6672792.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            31671..31766
                     /gene="mgtS"
                     /locus_tag="QBF46_000563"
     CDS             31671..31766
                     /gene="mgtS"
                     /locus_tag="QBF46_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDQ6672793.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(31985..32875)
                     /gene="dgcZ"
                     /locus_tag="QBF46_000564"
     CDS             complement(31985..32875)
                     /gene="dgcZ"
                     /locus_tag="QBF46_000564"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDQ6672794.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(33130..33522)
                     /gene="ydeI"
                     /locus_tag="QBF46_000565"
     CDS             complement(33130..33522)
                     /gene="ydeI"
                     /locus_tag="QBF46_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDQ6672795.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKVEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            33798..34316
                     /locus_tag="QBF46_000566"
     CDS             33798..34316
                     /locus_tag="QBF46_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDQ6672796.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
     gene            complement(34361..36406)
                     /gene="dcp"
                     /locus_tag="QBF46_000567"
     CDS             complement(34361..36406)
                     /gene="dcp"
                     /locus_tag="QBF46_000567"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDQ6672797.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRTEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRREFLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVENRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            36543..37289
                     /gene="ydfG"
                     /locus_tag="QBF46_000568"
     CDS             36543..37289
                     /gene="ydfG"
                     /locus_tag="QBF46_000568"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDQ6672798.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKVEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            37378..38064
                     /gene="ydfH"
                     /locus_tag="QBF46_000569"
     CDS             37378..38064
                     /gene="ydfH"
                     /locus_tag="QBF46_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDQ6672799.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            38241..38444
                     /gene="ydfZ"
                     /locus_tag="QBF46_000570"
     CDS             38241..38444
                     /gene="ydfZ"
                     /locus_tag="QBF46_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDQ6672800.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(38480..39940)
                     /locus_tag="QBF46_000571"
     CDS             complement(38480..39940)
                     /locus_tag="QBF46_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDQ6672801.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQFMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQENPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(40029..41312)
                     /gene="ydfJ"
                     /locus_tag="QBF46_000572"
                     /pseudo
     CDS             complement(40029..41312)
                     /gene="ydfJ"
                     /locus_tag="QBF46_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            41915..42028
                     /locus_tag="QBF46_000573"
     CDS             41915..42028
                     /locus_tag="QBF46_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672802.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            42097..42330
                     /gene="ydfK"
                     /locus_tag="QBF46_000574"
     CDS             42097..42330
                     /gene="ydfK"
                     /locus_tag="QBF46_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HDQ6672803.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            42647..43237
                     /locus_tag="QBF46_000575"
     CDS             42647..43237
                     /locus_tag="QBF46_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6672804.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(43335..43910)
                     /locus_tag="QBF46_000576"
     CDS             complement(43335..43910)
                     /locus_tag="QBF46_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ6672805.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKMVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTVWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(43910..47260)
                     /locus_tag="QBF46_000577"
     CDS             complement(43910..47260)
                     /locus_tag="QBF46_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDQ6672806.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKVSEASKSAAAAESSKSAAATSASAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSATSSATAAGNSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIYNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSRDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGAHTHSMTFVSG
                     GSSGAPGSGASDYSKYSVNTSSAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGSH
                     GHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(47325..47924)
                     /locus_tag="QBF46_000578"
     CDS             complement(47325..47924)
                     /locus_tag="QBF46_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDQ6672807.1"
                     /translation="MRKVCAAILSTAICLAVSGAPAWASEQQATLSAGYLNARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSVYAMAGVAYSRVSTFFGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(47992..51471)
                     /locus_tag="QBF46_000579"
     CDS             complement(47992..51471)
                     /locus_tag="QBF46_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDQ6672808.1"
                     /translation="MGKGSSKGHTPREAKDNLKTTQLLSVIDAISEGPIEGPVEGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSLTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPRL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEVIVDNGAHFDGEQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIRPGHNYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTVG
                     KAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLI
                     VKKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDWYPTASISDLLV
                     VVMKKATAGISIS"
     gene            complement(51532..52134)
                     /locus_tag="QBF46_000580"
     CDS             complement(51532..52134)
                     /locus_tag="QBF46_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDQ6672809.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAILALATQLPSFRQKLNE
                     GWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(52071..52814)
                     /locus_tag="QBF46_000581"
     CDS             complement(52071..52814)
                     /locus_tag="QBF46_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6672810.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMAPEDWLRAEMQGEIVALVHSHPGALPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            complement(52820..53518)
                     /locus_tag="QBF46_000582"
     CDS             complement(52820..53518)
                     /locus_tag="QBF46_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDQ6672811.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTEMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(53518..53847)
                     /locus_tag="QBF46_000583"
     CDS             complement(53518..53847)
                     /locus_tag="QBF46_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6672812.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(53844..56423)
                     /locus_tag="QBF46_000584"
     CDS             complement(53844..56423)
                     /locus_tag="QBF46_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDQ6672813.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDLLRGNWMAGLRSGWSEWKESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGINFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQAQMRDGGLFSG
                     GGR"
     gene            complement(56416..56850)
                     /locus_tag="QBF46_000585"
     CDS             complement(56416..56850)
                     /locus_tag="QBF46_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDQ6672814.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(56832..57254)
                     /locus_tag="QBF46_000586"
     CDS             complement(56832..57254)
                     /locus_tag="QBF46_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDQ6672815.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPKKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            complement(57270..58010)
                     /locus_tag="QBF46_000587"
     CDS             complement(57270..58010)
                     /locus_tag="QBF46_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6672816.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            complement(58018..58413)
                     /locus_tag="QBF46_000588"
     CDS             complement(58018..58413)
                     /locus_tag="QBF46_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6672817.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VTSGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(58410..58988)
                     /locus_tag="QBF46_000589"
     CDS             complement(58410..58988)
                     /locus_tag="QBF46_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6672818.1"
                     /translation="MAIKGLEQAVENLSRIRRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(59000..59353)
                     /locus_tag="QBF46_000590"
     CDS             complement(59000..59353)
                     /locus_tag="QBF46_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDQ6672819.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(59365..59760)
                     /locus_tag="QBF46_000591"
     CDS             complement(59365..59760)
                     /locus_tag="QBF46_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDQ6672820.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEEIDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVSAEMTERGLARMQ"
     gene            complement(59802..60827)
                     /locus_tag="QBF46_000592"
     CDS             complement(59802..60827)
                     /locus_tag="QBF46_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDQ6672821.1"
                     /translation="MSMYTTAQLLAANEKKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(60883..61215)
                     /locus_tag="QBF46_000593"
     CDS             complement(60883..61215)
                     /locus_tag="QBF46_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDQ6672822.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLMFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(61225..62544)
                     /locus_tag="QBF46_000594"
     CDS             complement(61225..62544)
                     /locus_tag="QBF46_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDQ6672823.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(62525..64126)
                     /locus_tag="QBF46_000595"
     CDS             complement(62525..64126)
                     /locus_tag="QBF46_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ6672824.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(64123..64329)
                     /locus_tag="QBF46_000596"
     CDS             complement(64123..64329)
                     /locus_tag="QBF46_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDQ6672825.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(64326..66251)
                     /locus_tag="QBF46_000597"
     CDS             complement(64326..66251)
                     /locus_tag="QBF46_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDQ6672826.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYVREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLQRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            complement(66226..66771)
                     /locus_tag="QBF46_000598"
     CDS             complement(66226..66771)
                     /locus_tag="QBF46_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDQ6672827.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            67160..67393
                     /locus_tag="QBF46_000599"
     CDS             67160..67393
                     /locus_tag="QBF46_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDQ6672828.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
     gene            67451..67861
                     /locus_tag="QBF46_000600"
     CDS             67451..67861
                     /locus_tag="QBF46_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDQ6672829.1"
                     /translation="MDQVVIFKQIFDKVRNDLNYQWFYSELKRHNVSHYIYYLATENV
                     HIVLKNDNTVLLKGLKNIVSVKFSKDRHLIETTSNKLKSREITFQEYRRNLAKAGVFR
                     WVTNIHEQKRYYYTFDNSLLFTESIQKTTQILPR"
     gene            complement(68013..68186)
                     /locus_tag="QBF46_000601"
     CDS             complement(68013..68186)
                     /locus_tag="QBF46_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDQ6672830.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDVKINLI"
     gene            complement(68358..68513)
                     /locus_tag="QBF46_000602"
     CDS             complement(68358..68513)
                     /locus_tag="QBF46_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672831.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(68593..68658)
                     /locus_tag="QBF46_000603"
     CDS             complement(68593..68658)
                     /locus_tag="QBF46_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672832.1"
                     /translation="MKAPSGAFWLGVYSMDTHILR"
     gene            complement(68661..68849)
                     /locus_tag="QBF46_000604"
     CDS             complement(68661..68849)
                     /locus_tag="QBF46_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDQ6672833.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(68860..69012)
                     /locus_tag="QBF46_000605"
     CDS             complement(68860..69012)
                     /locus_tag="QBF46_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_123282393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDQ6672834.1"
                     /translation="MTPKDGSKDVFVHFSAIQSNDFKTLTENQEVEFGIENGPKGPAA
                     VHVVAL"
BASE COUNT        16351 a        18877 c        17023 g        16766 t
ORIGIN      
        1 tattcgttat aaaataaggg ttatgcttaa tgctttaaga aaaatagcaa tttccccttg
       61 aatatattac tggtgatgga aggatgaatc tgcaagctgg catttaacaa gccagttcta
      121 aaagaaggga aataagaata actatactca aaaactaaca gccacggtca tcatgatgtg
      181 gctgtcaatg aaactataac ccaggcgctt tccataacga cgtcgtcagt ccatgatcaa
      241 ccagccccag ctgatcctga taaactgcct gccacttatc gcgtgaatcc tgataaagtt
      301 catgcttttc cgggtctggt gtgtgcgtcc gttcccagcg aaccaggcgt tctccggttt
      361 ctgccattga tgaaaaaatt ccggcaccga cgccagctgc aatggcacat cctaatgcag
      421 tggcttcttt gaccaccgga atattgacgg gtaatcccga gacatcagcg agaatttgac
      481 tccataattt cccttttgaa cctccgcctg caaagactaa cgatgaagga tgaatattgg
      541 agaaatcagc aatttgctgc aagttacacg ctgatacaat cgccgcattt tcttccagcg
      601 cacggaacaa tgtcgctttg ttacatttat ccgggtcaat ggacaagtta ataaaggaag
      661 gcgcagcgtg ataccaggtt ttaaagcgca ttctgtcgga gaatatcggc attacgcccc
      721 acgacccagg cggcacccgg ctggccatct cttccaacag cgtataggtg tcgatgccta
      781 gacgttcggc aatcagtttt tcttcggcac agaaagcatc gcggaaccag cgcatggtga
      841 gtccggtaaa aaagcttata gattcagctt gtaccatgcc aggaataaca tggggattaa
      901 cgcgcacgtt catttctgga tcggtcaccg gcgcggctaa gtttacaact tgctgccaga
      961 atgtgccgcc aagaacagcg gtttgtgccg gacgcacaac gcctaacccc aggcaaccaa
     1021 gctgcacgtc acctcctcca acgaccaccg gagtgcccgc cttcagaccg cagagttccg
     1081 ccgcttgtga actcaccaca cccagcaatg tgccggtttc tttgacagga gaaagaatat
     1141 cggcacgcag gccagccata tccagcaatg caggtttcca gtcacgggtg gttagatcaa
     1201 gaagtcccgt ggtgccagca ttagagggat ccaccgcaag ttcgccgctg agcatatagg
     1261 ccagccagtc gctgatcatg gtgatggttg atgcctgacg gtaaatatcg gaacgatggt
     1321 gcgccagcca aagtaatctg gggatggcac ttaaagccag tgtttgtccg gtcgcgcgat
     1381 aaacttcgtt ttcaaaggta ttgttgtgca gttctttaag ttcgctaact tcgcgcgccg
     1441 ctctggcatc cacattggcg caggcccaga tcggtgttcc ttcattgtta tataaaacaa
     1501 tgccttcacg catcgaacat gccgaaacgg cagcgatata ctccggggct atgccggcgt
     1561 tgtgcaacgc ctggcgcata cactcacacg ccagttgcca gtttttgttg agatcaaatt
     1621 ccatagaacc aggaacgtcc ggtactgcca gatgccgcca ctccgcctgt cccactgcta
     1681 tttgattgcc ttccaggtcg aatatcacag cccgaatact tccggtgcct gcatccagcg
     1741 ccatcaggta gtactttgat tctgaagggg taaagagtcg agccattaga ttatcctcgg
     1801 cttatgcgtt ctgtctgttc ctctatacgt tctccatcat tcccggtaat aaggtcatgc
     1861 aaatttaact acgtaaaatc gccgctgctg tgtcctgatc ggtaaccagt gcgttgatat
     1921 aaccgccttt cattgcagcg gcaattgctt cggctttatt ttctccccct gccacgccaa
     1981 cccggacggg tatggtcttc agcgcgctta aaggtaagcc aatcagttcg ttatgtattt
     2041 tgatatccgt gacaacgtca ccttttgcat caaaaaagta gcctaaaatg tcgccaaccg
     2101 ccccttttcg gccaatcatt aactgttcgc cctggctgat ataaccggag cgaatgattg
     2161 tcgcgtcgtc ttgttgactc acggccccaa tgccgacaat cgccacatcc gctgcttgcg
     2221 cggctaacag aacatctttg acgcaatttt cattttttag cgtacgggca atgtcagcgg
     2281 aggatgcccg caacggggcc ggaataatat tcacactgca cgccgcgtta agctgcccga
     2341 ttcccgtcat ataagaaccg acgccaccgg agagcgtgac caggcgaatt tgctgtgacg
     2401 aaataaaacc acttaagcgt tgcagcgtat tcatggttgc ctcgccaaaa ccaatcgcca
     2461 gcatctgttg tggttgaagt aaactcatca acatatgcgc cgcgcctatc cccagtcgcc
     2521 caccgacatc agcatccgca agcccaggga tcacccggac atgttgcagc gaaaactgac
     2581 gacgtaattg agtttcatat tccagacagc cttcaaagcg agaattaatc tgtacgcgaa
     2641 taatgccgga ctgatgccct ttctccagca atcgcgacac tttcaaacgt gtcaggccga
     2701 gacgatcgct gatctcgctc tgggtcagcc cgtcgtgata gtaaaaccac gcgatccgcg
     2761 cgacctgttc ttcttcacac attccctgtt ctgaaattgc cgaatcgttg attgtcataa
     2821 ttcattcttc actttgaaca tatttaaatc tttaatgcaa ttgttcagtt cttgctcatt
     2881 tatatctgtg atggcaacca cagtttgact ctacgagcat gaacaaacac aaccgtgaaa
     2941 atcaaaatag cataaattgt gatctattcg tcggaaatat gtgcaatgtc cacctaaggt
     3001 tatgaacaaa ttaaaagcag aaatacattt gttcaaaact cacctgcaaa actgaacggg
     3061 ggaaatatgc aaacgagtga tacccgcgcg ttaccgctac tttgcgcccg ctcggtttat
     3121 aaacagtatt caggggtcaa tgtcctgaaa ggcatcgatt ttacgttgca tcagggggag
     3181 gtccacgccc tgctcggcgg caatggtgcc ggtaaatcga cgttaatgaa gattattgcc
     3241 ggtattaccc ctgctgatag cggtacgctg gagattgagg gcaacaacta cgtcagatta
     3301 acgccagttc atgctcatca gctgggtatt tatctcgttc cccaggaacc gctgcttttc
     3361 ccaagcctgt cgataaaaga aaacatcctg tttgggctgg caaaaaaaca gctctccatg
     3421 cagaaaatga agaacttgct ggcggcgctg ggctgccagt ttgatctgca tagtctggca
     3481 ggatcgcggg atgtcgccga tcgccaaatg gtggaaatcc tccgcgggct gatgcgcgac
     3541 tcgcggattc tgatcctcga tgaacctacc gcctcgctta cccctgcgga aaccgaacgc
     3601 ttgtttagtc gcttgcaaga gctgcttgct actggcgtgg gtattgtttt tatctcgcat
     3661 aagctgccgg aaattcgcca gattgccgat cgaattagcg tgatgcgcga cggaaccatc
     3721 gccttaagcg gcaaaaccag cgaactgtct accgacgaca ttattcaggc catcacccca
     3781 gcggtacggg aaaaatcgct ctctgccagc caaaaattat ggctggagtt acctggtaac
     3841 cgcccacaac atgccgccgg aacgccggtg ctgacactgg aaaatctgac cggcgaaggt
     3901 ttcaggaatg tcagcctgac gctcaatgcc ggagaaattc tgggcctggc tgggctggtg
     3961 ggggccggac gcacagaact ggccgagacg ctctatggtc tgcgtacttt gcgtggcgga
     4021 cgcattatgc tgaatggtaa agagatcaat aaattatcca ctggagaacg tttactgcgc
     4081 ggtctggttt atctgccgga agatcgccag tcatccggac tgaatctcga tgcttcgctg
     4141 gcctggaacg tctgcgccct tactcataac cttcgtggat tctgggcgaa aaccgcgaaa
     4201 gataatgcca ccctggaacg ttatcgtcgg gcgctgaata ttaaattcaa ccaaccggaa
     4261 caagctgcac ggacattatc cggtggcaac cagcaaaaaa tcctcattgc caaatgcctg
     4321 gaagcttcgc cgcaagtatt gattgtcgat gagccgacgc gcggcgtgga tgtctcggcc
     4381 cgtaatgata tctaccagct gttgcgcagc atcgccgcac aaaatgtggc tgtgctgctt
     4441 atctcctccg acctggaaga gatcgaactg atggcagatc gtgtgtatgt gatgcatcag
     4501 ggcgaaatta cccactctgc actgaccgag cgcgatatta atgtcgagac tattatgcgc
     4561 gttgccttcg gcgatagtca gcgtcaggag gcgtcatgct gaagtttatt cagaacaacc
     4621 gtgaaatcac ggcactgctg gcggtggtgc tgctgtttgt attacccggt tttctcgacc
     4681 gccagtattt aagtgtgcaa acgctgacca tggtttatag cagcgcgcaa atcctgatcc
     4741 tcctggcaat gggcgcgacg ctggtaatgc ttacgcgcaa tattgatgtt tcagtgggtt
     4801 cgattaccgg aatgtgcgcg gtgctgttgg ggatgttact gaacgcagga tattcactac
     4861 ctgttgcttg tgtcgcgact ttactgcttg gtttgctcgc gggatttttc aacggtgtcc
     4921 tggtcgcgtg gctaaagatc cctgccattg ttgccaccct tggcacgtta gggttgtaca
     4981 gaggcatcat gttgctgtgg actggcggca aatggattga agggttaccc gccgaactga
     5041 aacagctctc cgccccgctg ctgcttggcg tttcagcaat tggttggttg acgataattc
     5101 tggtggcatt tatggcctgg ctgctggcaa agacggcgtt tggacgcagt ttttatgcca
     5161 cgggcgataa tttacagggc gctcgtcaac tgggcgttcg tactgaagcc attcgcattg
     5221 tggcattttc gttgaacggt tgcatggcgg cactggcggg aattgtgttt gcttcgcaga
     5281 ttggttttat ccccaaccag accggtaccg ggctggagat gaaagcaatt gcagcctgcg
     5341 tgctgggcgg cattagtttg ctcggcggtt ccggtgcgat catcggtgcg gtactcggcg
     5401 catggttcct gacgcagatc gatagcgtac tggtgctgtt gcgcattccg gcatggtgga
     5461 atgattttat cgcgggtctg gttctgctgg cggtgctggt gtttgatgga cgcctgcgtt
     5521 gtgcgctgga acgtaatctg cggcggcaaa aatatgcccg ctttatgacg ccaccgccat
     5581 ccgttaaacc cgcttcgtca ggtaaaaaac gggaggctgc ataatgcgta ttcgctacgg
     5641 ttgggaactg gctcttgccg cactgctcgt aattgagatt gtcgcatttg gtgcaattaa
     5701 cccgcggatg ttagacctca atatgttgct gttcagcacc agtgacttta tctgcattgg
     5761 cattgtcgcc ctaccgctga cgatggtgat tgtcagtggc gggatcgata tttcgtttgg
     5821 ttcgaccatc ggcctctgcg ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat
     5881 gccgctggcg attctcctga ccttactgct cggcgcattg tgcgggctga tcaacgccgg
     5941 attaattatc tataccaaag ttaacccgct ggtgattacg cttggcacgc tgtatctgtt
     6001 tgccggaagc gctctgctgc tttccggtat ggccggagcg acggggtacg aaggtattgg
     6061 tggattcccg atggcgttta cagatttcgc taacctggat gtgctgggac tccccgttcc
     6121 gctgattatc ttcctgatat gtctcctcgt tttctggctc tggctgcata aaacccatgc
     6181 cggacgtaat gtgtttttga ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc
     6241 agttaaccgc accttatgtg cgctctatgc catgacgggg ctggcgtctg cggtcgccgc
     6301 tatgctgctg gtatcgtatt ttggttcagc acgttccgat ctcggtgcgt cgtttctgat
     6361 gcccgccatc accgccgtgg tgcttggcgg ggccaatatt tatggtggtt ccggttccat
     6421 tatcggcacc gccattgcgg ttttattagt gggatatttg caacaaggtt tgcaaatggc
     6481 aggagtgcca aatcaggtgt ccagcgccct ttccggtgcg ctacttatcg tcgttgtcgt
     6541 aggtcgttcc gttagcctgc atcgccagca aattaaagag tggctggcgc gtcgggccaa
     6601 taacccattg ccataaagga tatcttcatg acacttcatc gctttaagaa aatcgcctta
     6661 cttagcgctc ttggcattgc cgcaatctct atgaatgtgc aggccgcaga gcgtattgca
     6721 tttattccca aactggttgg cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa
     6781 gcgggtaaag agctgggcgt tgatgtgacc tacgacgggc cgacagaacc cagtgtttct
     6841 ggtcaggtac agttgattaa taacttcgtc aatcagggtt ataacgccat tatcgtttct
     6901 gcggtttcgc ctgatggctt gtgtccggca ctgaaacgcg ccatgcaacg tggtgtgaga
     6961 gtgctgacct gggactctga tactaaaccg gagtgccgct cttactacat taatcaggga
     7021 acgcccgccc agttgggagg tatgttggtg gatatggcgg cgcgtcaggt gaataaagac
     7081 aaagccaaag tcgcgttttt ctactcaagc cccaccgtta cggaccaaaa ccagtgggtg
     7141 aaagaagcga aagcgaaaat cgccaaagag catcctggct gggaaattgt cactacgcag
     7201 tttggctata acgatgccac taaatcatta caaaccgcag aaggaatatt aaaagcgtat
     7261 agcgatctcg acgccattat cgcccccgat gccaacgccc tgcccgctgc cgcacaagcc
     7321 gcagaaaact tgaaaaatga caaagtagcg attgtcggat tcagtacgcc aaacgtgatg
     7381 cgtccatatg tggaacgcgg cacggtgaaa gaatttggcc tgtgggatgt ggttcagcaa
     7441 ggcaaaattt cagtgtatgt cgcggatgca ttattgaaaa aaggatcaat gaaaacgggc
     7501 gacaagctgg atatccaggg cgtaggtcag gttgaagtct cgccaaatag cgttcagggc
     7561 tatgactacg aagcggatgg taatggcatc gtactgttac cggagcgcgt gatattcaac
     7621 aaagagaata tcggcaaata cgatttctga tgtgcattac ttaaccggag taagttatgg
     7681 cagatttaga cgatattaaa gatggtaaag attttcgtac cgatcaaccg caacaaaata
     7741 tcccttttac cctgaaaggt tgcggtgcgc tggattgggg aatgcagtca cgcttatcgc
     7801 ggatatttaa tccgaaaacg ggtaataccg tgatgctggc ctttgatcat ggttattttc
     7861 aggggccgac taccggactt gaacgcattg atataaatat cgccccgctg tttgaacatg
     7921 ccgatgtatt aatgtgtacg cgcggcattt tgcgcagcgt agttccccca gcgaccaata
     7981 agccggtggt actgcgggcg tcaggtgcga actctattct ggcggaatta agtaatgaag
     8041 ccgtggcgtt atcgatggat gatgccatgc gcctgaacag ttgcgcggtg gcggcgcagg
     8101 tttatatcgg cagcgaatat gaacatcagt cgatcaaaaa tattattcag ctggttgatg
     8161 ccggaatgaa agtgggaatg ccgaccatgg ccgtgactgg cgtgggcaaa gatatggtgc
     8221 gcgatcagcg ttatttctcg ctcgcgactc gaatcgccgc tgaaatgggg gcgcaaatta
     8281 ttaaaaccta ttatgtcgaa aaaggttttg aacggattgt tgccggatgt ccggtaccca
     8341 ttgttattgc tggcggtaaa aaattaccgg agcgcgaggc gctggaaatg tgctggcagg
     8401 ctatcgatca gggcgcttct ggtgtggata tggggcgtaa tattttccag tctgaccatc
     8461 cggtggcgat gatgaaagcc gtacaggcgg tggttcacca taacgaaacg gctgatcggg
     8521 catatgaact ctatctgagt gaaaaacagt aactgcggat ctaaggagaa gaattatgca
     8581 cgtcacactg gttgaaatta acgttcatga agacaaggtt gacgagttta tcgaagtttt
     8641 tcgccagaac cacctgggct ctgtacagga agaaggcaat ttgcgcttcg atgtcttaca
     8701 ggacccggaa gtgaattcgc gcttttatat ctacgaagcc tataaagatg aagacgcagt
     8761 ggcgttccat aaaaccaccc cccactacaa aacctgtgtc gcgaaactgg aatctttaat
     8821 gaccgggccg cgtaaaaaac gtctgttcaa tggtttgatg ccgtgaggcg aatttatcaa
     8881 ttttatctac aattggggta acgcgctgac gggagtaaaa aaatgtctga ctggaacccc
     8941 tctttatatt tgcacttttc cgctgaacga tcgcgtccgg cggtggagct gcttgccaga
     9001 gtgccgctgg aaaatgtcga atatgttgcc gatcttggct gtggcccagg taacagcacc
     9061 gcccttctac aacaacgttg gcctgcggcc aggataacag gcatcgactc gtctccggcg
     9121 atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc agacatccgc
     9181 aactggcaac cggtacaggc actcgatctg atttttgcta atgcctcact gcaatggctg
     9241 cccgaccact acgaattgtt tcctcatctg gtttctttac ttaatccgca gggcgtgctg
     9301 gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg cgaagttgcc
     9361 tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca tgcttactac
     9421 gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccaccta ctatcaccag
     9481 atgccgtcac accaggcgat tatcgattgg gtgactgcca ctggattacg tccgtggtta
     9541 caggatctga ccgagagcga acagcagctt tttcttaagc gctaccatca gatgctggaa
     9601 gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg tctttttatt
     9661 gttgcccggc gtatggagta aatatcacgt cagctggtaa tgacgatcgg gaagaatttt
     9721 tgctggaatt tcggcttcat cgttcatctg taacaggtca atttcaatag cgttgcagat
     9781 ggcatccagt ggtaaatcat tgttttcagt accgaacgga tcttccagtt cttccgccag
     9841 acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg tcatgtaatg
     9901 caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg tacgatgcaa
     9961 aatcagggtg taggcaaagg gaattggcgt ataggcaatg cgctcgcatc ctgccaggac
    10021 cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg aaagctgtcc
    10081 attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt tagctggaga
    10141 gttcgaagcc agtacacgct gaagatcttc agtcttgaga taatgagcca gcacttccgc
    10201 ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt gcagccgggc
    10261 aaactccctt acacttgccg aatccggcaa tgtcgttttt acctcgcgca gtaacgaccg
    10321 tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg cgtacccggc
    10381 attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga acggtgcgag
    10441 ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag cgatagaaaa
    10501 gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt gccagacaaa
    10561 aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca ggcgtgaaaa
    10621 agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac aaattaccta
    10681 caagcttaca acattaacag gcgctgcatg tggcacctgt taatgataaa ggcatatagg
    10741 atgttgtaac taatatggtg aaataagaaa cccggtcgaa accgggttca gaagtaacgg
    10801 tgttattagc acaacggacg tacagcttcg cgcatccctt tttcgagaat cgcatccagg
    10861 tcattagcaa cctgctcgac cagaccaggc acttgcgtca ggtcctgctc ccagtggtct
    10921 ttttctgcca aaacaatcgc taccagttcc tgggtgccga taacacggtc acgatgctgg
    10981 ctccagagtt gctgataacg ttccagccag tgtgcatcgt cctgtaccgg atatgtttcg
    11041 ccgttacgct caccacgata gaacgcaatc aatgccgcta atgcgaaagt aaggcgcgcc
    11101 ggaagtgtgc cgtttgcctt ctgccctgcc agcagctgtg gcaggatgcg ggtgcggaac
    11161 ttggtcatac cgttgagcgc gatagacagc agctgatgct taatgtacgg gttacggaaa
    11221 cgcccggtga ctgcactggc gaaagattcc agttcatcac gaggcaaatc cagtaccgga
    11281 ataatttctt cgtagatagc tttttcaacg aatgcgcaaa tttcagcatc gttcatcgct
    11341 tcacctacgg tatccagccc tgcctggaac gccaccggca ccagcgcggt gtgcgcaccg
    11401 ttgaggattg ccactttgcg ttctttgtac ggtttaatat cgtcaacaat cagcacgttg
    11461 agcggatatt tgtccagacg cagttcagtc gctaaggatt tcggtccctg aatcacaaac
    11521 aggtaaaagt gttcagcggt gtcgagaaaa ccatcgtgat aacccagttc ttcttccagt
    11581 ttagccactt catcgcgcgg ataaccggta acgatacggt ccaccagcgt agaacagaag
    11641 ctgttagcct gatccagcca ttgaataaat gcttctggca gtgcccactc ttgcgcatag
    11701 cgcagcacca gttcacgcaa cgcgtcgcca ttgtagtcaa tcaactcaca cggaatgatg
    11761 atccaacctt tatccagcgc accgttgaaa tggctaaagc gttcgaacag cagacgggtc
    11821 agttttgccg gatagcttac cgctggcgcg tcatcgaatt tatcgcccgc atggtagctg
    11881 atacccgctt cggtggtgtt agagaaaaca aagcgcattt ccgggttgtg cgccagtttc
    11941 aggaattcat cgtattcact gtagacgctg atttcacgat taaccgagcg aatcagacgc
    12001 gcgtcgctga ccgcttcccc cttctcattc aggccacgga taatggtggt gtacagacca
    12061 tcctgcgtgc tcagtgacgg cgggaatgaa gtttcaatcg gacgaacaac gaccacacca
    12121 gaattcagat cggtgtgctc attcaggaga tcgatttgcc agtcaacaaa ggcgcgcagg
    12181 aagttacctt caccaaactg aatgatacgt tctggatact gtgcaccggg aaaatcgcga
    12241 cgatttagtg ttttcacaat gggttccctt ctgattagtc atacaacctg tttgaattgg
    12301 tacgacaggt tagcaaactt taatacgccg aacccctgtt ttgatcaact cctgatgatt
    12361 aatgagcagt tttatgagaa aagtgtggcg cggatcatgg tttaatcgag gaaatacgcc
    12421 ttttcctgga tcataaagtg gtagaacaca ttgcattcaa atcgcgcgga atgaataaag
    12481 atgtcagaca acttcctcac cgtaacgcat agtgctggta cggttgcgcc catctttctt
    12541 cgagcgatac agacacgtat cggcttcgac catcaattta ttaaagtcat cggtcagcgt
    12601 tcggtacgat gcgcgaccac taccgacgcc aatacttacc gtgagataaa gcgttttttg
    12661 ttgccaggtg aatggttgca gttcaacgcc tttacggatt ttttccgcca ttagcagacc
    12721 atcgacagga ttcaccgacg gcactgcaac agcaaattct tcgccgccca ttcgcgccac
    12781 cagcccctta tcaccgacaa tcttctgaat atgccgggca aacacgctta acactttatc
    12841 gccacattca tgtccatagt tatcgttaat gcttttgaaa tagtcgatat caagcagcat
    12901 gacagtcaga tgttgtgtct gtttcagcga tagacttttc agcgcctcat aaagaccgga
    12961 ccgtgagtaa acctgagtca gaaaatcaaa gtcggctcgc agcgcaactt gcttcattag
    13021 cgaattgatc gctgccacgc taaaagaaac cataattggg catatcgcca tcgtggcaat
    13081 accgagacgt gcggagaaca tttccggaat ggagaacggc gaaccgaccg agatatcaat
    13141 caccgaattt gccaccagta cgatttccac cgcaccggtg acaaaggtca acagacatgt
    13201 tacctgcggc gtatagcgca ctgcacacca gattaatgca ggcagcggga acgccagact
    13261 ccccgctcca ccaatgacta ccgaggcaat aaccgacaca ataagcgcga tggcgggcat
    13321 catctgttct gctttaaagc gcggcagcac tccaggaata gccaacgtca gcatacaagg
    13381 cacgatcaac acgcccgttg agaattgctc actgaaccag tcggcaagca aaggccagaa
    13441 atccagacta tcaatactga ccgaaccaat cgccccgaca atagcgcata ataatgcggc
    13501 aatcagacag taattaaata gccgtaaggc acttactggc tcatacttat ttttccccag
    13561 acgcttatcc cgcacgacca gtaaggcgac ggtaacaata aacatcatat tggagaaatt
    13621 gatagccagt gaaaccagtc cccattcggt ggttatggca tcataaacca gcatcgccac
    13681 ataactgatc gcatagtaat gcagtcgatt aagccagaca tagcgggcaa acacccccgc
    13741 cattacgcca ttcagcggcc agaaaagaga aagttcgtga accagccgta actctgcacc
    13801 aataaaatag aacagcgtgg ttagcgcaaa aatagcgatg ctattacgta gcagatgacc
    13861 ttcctggaac aaacggaaag ttgaaatggg gtgtacatgc attaattatc tgtccataca
    13921 attgcgggtg gcaattatct caacgtaata agtaaattct cataaaggat agcatgttcg
    13981 ctaacagcgg cggctaataa aggtaccctg ggaatgctat tttatcgttt tccgcaattc
    14041 tcatggctga ttcatctgta caaccagcca gtgatagatt accgccacga tataacgttg
    14101 ttgttcttcg cttaacgaaa caccctgcgg aatgttatgc cacttagcca gacaaccgcg
    14161 acagcaggtc gcggtggcgt gctgagcgat aaacaccgga tgcccgcgca tgggggtttg
    14221 cttaccgtca ttagccggta atgccggagc aagacgttta gcgacaaaat ccgccgcatg
    14281 ttgttcgatg actggcgcgc ctttctccag gcaatactga cgctctttca tgcccagacg
    14341 aaagcgagag cgaaattttg aacgggataa acgcgcgaag agtggatcga gagactgcat
    14401 taataaaccg aacgccctaa ctgtttcgtc aattgttcaa ggacggcaat acccgcaagc
    14461 gagttacctg catcatccag ttccggactc cagacagcga tggccatttc atgcggaaca
    14521 atcgccacaa taccgccacc aacgccagat ttcgccggta gccccacccg ccaggcaaac
    14581 tcccccgcgt tctggtacat accactggtc gccatcagcg cgttaatttg ccgcgcctgc
    14641 attggcgtca ccactggttc atcaatatga atagctttcc cctgattagc cagaaagaca
    14701 aacgtccggg ccagctctac acagctcatt ttcagagcgc agtaatgaaa gtagttttgc
    14761 agaacggttg tcacgtcatg atggaaattg ccaaacgact tcatcagcca ggcgatagcc
    14821 gcatttcgcg cggaatgttc aaattcggaa cgcgctacca ccgtatcgta ggaaatatca
    14881 gacacaccgc ttaagccgcg cacgacttcc agcatacgtt gccgtggtgc gcttaatcgc
    14941 ccttgcaaca tatcgcagac caccagcgca ccggcattaa tgaacggatt acgcggtata
    15001 ccctgctcca tttccagttg cactaaggaa ttgaacggtg atccagacgg atctttgccg
    15061 acgcgttgcc agatttcctc ttcggagtaa tgacgcatgg cgacaacgag actcagcact
    15121 ttggaaatag actgaatgga aaaacgttct tgcgcgtctc cggcctgaaa aagctgtccg
    15181 tcaacggtac agatagcaat ccccaatcgg gaaccgtcta ctgtagccag cgccggaata
    15241 taatccgcga ctttaccctg accaatgagc ggacgcactt gccgcaagat gttttctaaa
    15301 attgcattat ccatggcgac tgccactttc tactcctgga ccgcaggtct gaaaagacct
    15361 gcgagtatat cagagctgaa tatgtcgcgt cagatccggt ctttccacac cgtctggata
    15421 ttacagaatt cgtgtaagcc gaaatgggaa agctcacgac caaagccact ctttttcacg
    15481 ccaccaaagg ccactcgcgc gtcgctggca caataaccat tgataaacac cccaccgcat
    15541 tccagacgtg ccgccatctg tctggcctgt gtttcgtcag tggtaaaaat ggtcgctgaa
    15601 aggccgaact cactatcatt agccagttcc agtgcatgtt ctgcatcttt cgcaatggtg
    15661 attgccgcaa cggggccaaa catttcttcc cgaaacgcgg tcatttctgg ggtaacattc
    15721 gccagaaccg ttggcggata gtagttacct gccccagcca tcttttcccc gcccagtaac
    15781 aaacgcgcac cctgcgccag ggttttctcc acctgatgat gcagctcatc acgtaaatca
    15841 aaacgagcca ttggtccgag agcgttctct tcgtcacggg gatcgcccat tttcaaggct
    15901 gccgcagctg ccacaaaacg ttcggtaaat gccgaagcaa ttccctcttc gataataaag
    15961 cgttttgccg ctgcacatac ctgtccggta ttctgataac gtccggctac cgccgctttc
    16021 accgccagtt ccagatcggc atcgttaagc acaataaacg gatccgaacc gcccagttcc
    16081 agtacgcatt ttttcagtgc cgctccagcc tgtgcgccaa tagccgctcc cgcacgaaca
    16141 cttccggtca ccgtgacagc agcaatgcgc gagtctttaa tcatctgact gacaccgtcg
    16201 ttgtcggcat tcagccagcc atatacgcct tgtgggatac ccgcatcttt aaacacctgg
    16261 gcaatgagct gtgcacagcc catcacattc ggcgcatgtt taagtaagta gccgttacct
    16321 gcaagaatga tgggaacagc gccacgcatc acctgccata acggaaaatt ccacggcata
    16381 atcgccagaa tcgtccccaa cggtcgatac tcaataaccg cctgctgatt ttccaccagc
    16441 gtaggttccg ccttcagcat tgccggacca tgttctgcat accagtcaca caaattcgcc
    16501 gatttcgcca cttcagcgcg cgcctggttg attggtttgc ccatttcgcg ggtgatcatt
    16561 tgcgccattt cttcgctacg agcgcgcaga gccttaccga tatcacgcag tttttcagca
    16621 cgataatcta tatttgtctc gcgccagtcg cgaaagcctg ctgccgccag ctgaagtgcg
    16681 ttttcgatat cgtcagcgcc agcccacggc agcacagaaa gttgttcacc cgtggcagga
    16741 tttatcgaaa ttgcatgagt tgccggagta atggtcatcg gggtatctcc tttatgagtc
    16801 atggtatgaa gatacgcaga tttactcttg ctttaaaatg aataatatta agccacttat
    16861 tcacgaatcg agaatgctat ggatctgacc caactggaga tgttcaacgc ggttgccgaa
    16921 gctggcagca taacccaggc tgcagcaaaa gtgcatcgcg tgccgtcgaa tctcactacc
    16981 cgtctgcgcc agctggaaac agaactgggg gttgatctgt ttattcgcga gaatcagcgt
    17041 ttacgtctct ctcctgccgg gcataacttt ttacgctata gccaacaaat tctcgcgtta
    17101 gtggatgaag cgcggagcgt tgtcgctggc gatgagccgc aaggtttatt ttctcttggt
    17161 tcgctggaaa gcaccgctgc agtgcgcatt ccagccacgc tggcggaatt caaccgtcgt
    17221 tatcccaaaa ttcagttttc actttccacc ggcccttccg gcactatgct ggatggtgta
    17281 ctggagggaa aactgaatgc ggcgtttatt gatggaccca ttaaccatac tgccatcgac
    17341 gggataccgg tataccgcga ggaactgatg atcgtcacgc cacaaggata tgcgccagta
    17401 acccgtgcca gtcaggttaa tggcagtaac atttatgcct tccgcgccaa ttgttcgtat
    17461 cgtcgccact tcgagagctg gtttcatgct gacggtgccg ctccgggaac tatccatgag
    17521 atggagtctt atcacggaat gttggcctgt gtgatcgcag gagcaggcat tgcgcttatt
    17581 ccgcgctcta tgctggaaag tatgccgggg catcaccagg ttgaagcgtg gccgttagct
    17641 gagcaatggc gttggttaag gggtcgtctc agaaaacgga aaataaagca cgctaagccg
    17701 gttgcagcgg ccgtagcggc ctgaacttgc ccgcgccgat cttggcgctg ctgcgccaga
    17761 ggtaatcgcc ggtcaggttg atgtgctccc agccgagcgg cgacaggtac tgcaacaggc
    17821 cgtcatcgac ggcttgaccg tggccacgca aggcgttcgc ggcccgctcc agatagaccg
    17881 tgttccatag cacgatggcc gccgtcacca ggttgaggcc gctggcccgg tagcgctgct
    17941 gctcgaagct gcggtcgcgg atttccccca ggcggttgaa gaacacggcg cgggccagcg
    18001 cgttgcgcgc ctcgcctttg ttcagcccgg catgcacgcg gcggcgcagc tcgacgcttt
    18061 gcagccagtc caggatgaac agtgtgcgct cgatgcgtcc cagctcacgc agggcgacgg
    18121 ccaggccgtt ctggcgcggg tagctgccaa gcttcctgag catcagcgag gccgtcgccg
    18181 tgccctgctt gatcgaggtg gccatccgca ggatttcatc ccaatgggcg cggacgtgct
    18241 tgatgttgag cgtgccgccg atcatcggtt tcaacgcctc gtaggtggca tcgcccttcg
    18301 ggatgtagag cttggtgtcg cccaggtcac gaatgcgcgg ggcgaagcgg aagcccagca
    18361 ggtgcatcaa cgcgaagacg tggtccgtga accctgccgt gtcggtgtag tgctcctcga
    18421 tccgcaggtc ggattcgtga tacagcaggc cgtcgagcac gtaggtcgag tcgcgcacgc
    18481 cgacgttcac gaccttggtg tggaacggcg cgtactggtc ggagatatgg gtgtagaacg
    18541 tccgccctgg gctgctgccg tatttcggat tgatgtggcc ggtgctctcg gccttgctgc
    18601 cggtgcggaa gttctggccg tccgacgatg acgtggtgcc gtcgccccaa tgcccggcga
    18661 agggatgtcg gaactgcgcg ttgaccagct cggccagtgc cgccccgtag gtttcgtcgc
    18721 ggatgtgcca ggcttgcagc caggccagct tggcgtaggt cgtgccgggg cacgattccg
    18781 ccatcttggt caggcccagg ttgatcgcgt cggcgaggat cgtggtcagc aacaggtttt
    18841 tgtccttggc caggtcgcct gacttcaggt gggcgaagtg ccgggtgaag cccgtccatt
    18901 cgtctacctc cagcagcaat tcggtgatct tgacgtgcgg taggatcatc gccgtctggt
    18961 cgatcagggc ctgtgcggta tcgggcaccg ccgcatcgag cggcgtgatc ttcaggcccg
    19021 actccgtgat gatggcgtcc ggcagctcgt tggccagcgc catgcggttg acggtggcga
    19081 gctgcgtttc cagcagcgtc agccggtcat gcaggtactg gtcgcaatcg gtggccacgg
    19141 ccagcggcaa ttcgctggcc tgcttgaggc tggcgaattt cgcgggcggc accaggtagt
    19201 cctcgaagtc cttgaactgg cgcgagcctt gcacccagat gtcgccggag cgcagcgcgt
    19261 tcttcagctc cgacagcgcg cacagttcgt agtagcgccg gtcgatgccg gtgtcggtca
    19321 tcaccagctt ctgccagcgc ggcttgatga actcggtcgg cgcgtcggtg ggcaccttgc
    19381 gggcgttgtc gctgttcatg ctgcgcagca cctcgatggc gtcgagtacg tccttggcgg
    19441 cgggcgcggc ccgcaacttg agcacgtcga gaaattccgg cgcgtagcgg cgcagcgtgg
    19501 cgtagctctc gccgatgcgg tgcaggaaat cgaagtcctc gggttgcgcg agccgctgcg
    19561 cttcggtgac gctctcggcg aaagcatccc aggacatgac ggcctcgatg gcggcgaacg
    19621 gatcgcggcc cgcttgcttg gcctcgatca gcgcctggcc gatgcgcccg aacagccgca
    19681 ccttggcatt gatcgccttg ccggatgcct ggaactgctg ctgatgcttg ttcttggcgg
    19741 cattgaacag cttgcccagg atgcggtcat gcaggtcgat gatttcgtcg gtgacggtgg
    19801 ccatgccctc gatggcgagc gccaccaggg tcgcgtaacg ccgctgcggc tcgaacttcg
    19861 ccaggtcggc gggcgtcatc tggccgccct cgcgggcgat cttgagcagc cggttctggt
    19921 gaaccagccg ctcgatgccg gagggcaggt cgagcgcctg ccacgccttg aggcgttcga
    19981 tgtgttccag catgtgccgc gagttcggtt tgaccgggga ttgccgcagc caggccagcc
    20041 acgtcgtctt gccgttgtcg cggcgcttga gcagatcgtc gaggcgacgg cgatgcacgt
    20101 ccgtcagcgg ctcagccaag gcgtcgtaga gacgccggtt ggcgcgggta atcgcttcgg
    20161 cgctcgcccg ctcgacggcg ttgagggcgg gcacaatgac cgactgccgc cgcaggtgct
    20221 cgatcaaggc tctggccagc acgatgccct tgtcggtttg catggccagc tcggtcagca
    20281 actggacagc ctgccggtag tggccaatcg tgaacggctg gaagccgaac accgtttgca
    20341 gctcgaccag gtgctcgcgt cgggtctgct cacgctgccc gtactcgtcc cagctttcga
    20401 tgccgacctt gagctggttg gcgaccagtc tcagcaatgg cgggaacggt ggctcatcag
    20461 cgccaaggat gacgccggga aagcgcaggt agcagagctg caccgcgaag cccagccgat
    20521 tggccgggcc gcgccgctgc cggatgatgg agaggtcgct ttcgctgaac gtgtagtgac
    20581 ggatcaactc atccttggtg tccggcaacg ccagcaggct ttcgcgctcg gcggcggaga
    20641 ggatcgaacg gcggggcatg cggtttcctt cttcttgaaa acgtaggttt gtgacaaacc
    20701 cgccaaggca accggcgcgg cacgggaatc aaggcattgc gattctcgaa aatagttctt
    20761 gaaattctat tcttgattgc atatcatctc aacgagtttc gataagaaag gatgcgcatg
    20821 cgaccgtctg ttgtgcttga catgaagcga agcgcagtgc gtgaagcggt aggccgcttt
    20881 cgcgccgcga acccgcgcgt cttcggctcg gtgctgcatg gcaccgaccg ggatggcagc
    20941 gacctcgacc tgttggtcga tgcgctgccc ggtgccacgt tgttggactt gggcgatttg
    21001 gaagaagaac tgaaatcgct gctcggcgtt gacgtcgatc tgctgactcc cggcgacctg
    21061 ccgccgaagt tccgggccaa ggtgctcgcg gaggcgcaac cgatatgagc gagaaccgcc
    21121 tgcccgatta cctcgaccac attcagcagg ccgcaaccga tgcgcgcagc ttcgtggaag
    21181 ggatggccaa ggacgacttc ttggccgaca agcgcaccca gcaggccgtc atcatgagcc
    21241 tgatcgtcat cggcgaggcg gccacaaagg tgatggatgg ctacgtcgag ttcacccagg
    21301 cgcatgccga cgtgccgtgg cgcagcatgc gcaatatgcg taatcgcatg gctcacggct
    21361 atttcgacat caacctcgat gtggtgtggg agacggtaca ggaatggctg ccggcgttgc
    21421 tccagcaatt gcccgccgtg cgtcaggatg ccgacgatga agaccgtaac gacaactgtg
    21481 aaagagggat atccgatgac tgttgaatcg agaatatttt ctgtagccga gtatgttcag
    21541 ccgtccgaag gcgagcctat tcgttccgtt gtgcttgaaa cccgagactc aattatcgtg
    21601 gtttggcatg tccatcccgg gcaggaaatt gcggctcaca ttcatcctca cggccaagac
    21661 acgtggactg ttttgtcggg aatggctgat tactttcagg gcaatgggat tgttcgtgcc
    21721 ctcagggaag gtgagatagc cgtggcaaga ccgggccaag tgcacggggc gcgaaataca
    21781 ggtaccgagc catttgtgtt agtctcggtt gtggcatcag ccaatgccgg tttcgtattg
    21841 gctgagcgat agagcccaat ctctggagtt ggtccaatga gcggtcgggg agataggtaa
    21901 cagacatgca gcggacacgg ctgctaaacc aggtcgcaaa cctccttgcg tcgcagcgtg
    21961 ccgcaagcga cgcgatcaat cgaatggggt cggcatgaga ctgaacacca tccagttccc
    22021 gaccgcgtag ccgcctgtcc tgccgatcag gtcttgacca tcgacgcctg ggatcagtcc
    22081 tgaatgttct tggagaccac tacgttatga gccgcagccg ccgcaaaaca cccatcgtcg
    22141 ggcacacgac ctgcggcagc gagcgcgagg acaagaagct ctggcatcag cgctggcgca
    22201 cccgtgagcg cacggcgctg accagcgcgt cgcccgaagc cctgagcgcc catctgcccc
    22261 tgctggaaaa ccaggccagc agcgtctggt cgatgggcaa ggatggccgc tcctactggc
    22321 ccgtcaagcg ccaggccgcc acggcggatc gcatcgccaa tcacaaggga cgcaacccgc
    22381 aagaacgcgc ctccctgaaa aagcgcctgc tgcgcaagtg gatgagcaaa tgaagctctc
    22441 cttccatcag cacattgcgc tgttctggat gatcggtgct ccgggcgtct tcgcgcccgt
    22501 gatcgagaac gccaagcggc ccgatgccgg cgccgtcatg gcgtggggtg tcgcgatcgt
    22561 ggcggtgatg atcctcttca cccctttgct gctgcgctgt ccaccattcc ggcgctggta
    22621 tggccggacg gatgcgctgt cggagcggca gcgccaggcg cttgccgagc gcggcctgcg
    22681 ccgctactac cagaccgctt tcgatgacgg ctacgtgccc cgcgtgatgc cctacgtgtg
    22741 gcgcatcatc tggacggtcg gcgggttgat ggctgtgacc gccgtactgc ctaccaacac
    22801 cggacggcca gccttcgatg ccttggtggt cttctcgacc tggtatccga taggcgtgat
    22861 gctgctggtt ttcgcgtcga ggccccttgg ccggttgatt cgccaaagag cacaggagcg
    22921 gcggaaatga gcacgtcaac catcgaggcg ctggccagcg cctgggcaag gattgccgag
    22981 gaagcggaat tccccgctga ctacgagggg actgccacac cacaagcgca tcgggctagc
    23041 gaagctattc aggagcagat tcgggagcgc atcgtcgcca ccaacgacat gcggctgttc
    23101 agcctgctgc acctgctggg tcaggcgtcg ctgcgcatgg agcaagcgct gtggccggag
    23161 gattacgagc ggatgacgcg cgaggttgag gaagccctgc ggcaagccac cgacgccaac
    23221 gccagatcgt acacccacga agaagtgatg caggcgatgc aggaacgcat cgaccgggcg
    23281 cgagacaagc catgttgatt ggctatgcgc gcgtctcgac gcaggatcag aacctggagc
    23341 tgcaacgcga agccttgagc aaggccggat gtaaaaaggt cttcgaggac aaggtgagtg
    23401 gcacgcgggc agaccggcct ggcttggcca agacgctcga aatgctgcgc gaaggcgata
    23461 ctttggtcgt ctggaagctc gaccggctgg gccggtcggt caagcaactg gtcgatctgg
    23521 tcggcgatct gcacaagcac ggtgtccagt tcaggagcct caccgactcc atcgacaccg
    23581 gcacaccatc cgggcggttc ttcttccacg tcatggcgag ccttgccgaa atggagcgcg
    23641 agctgaccgt cgagcgcacc cgcgccgggc tggaagtcgc caagcagctc ggccgcaaag
    23701 gcggccgcaa gccgaagatg accgacagca agatcgagtc ggccaagaag ctgctggcca
    23761 gcggggtgcc gcccaaggac gtggccaaga acctcggcgt gtccattccg acgctgtacc
    23821 gctgggtgcc agcctccacg cacgcttagc gtgctttatt ttccgttttc tgagacgacc
    23881 ccgttaacaa cctggctggt ctggcgtcgt ggtgcgaaaa cgcgcccgct cgaggcattt
    23941 attcaactgc tggatgtgcc tgactcggca agacagggat atcaatgagc tatttttgat
    24001 agttcttgcg gctaatatgc tctatatagt gatgttccga tgacttatga ctatatgggg
    24061 caaatatggt tacgccagta agcatcagca attacatatc tcttcctgat gattttcccg
    24121 tacgtaatat tgcacctcag gtaaaagaag ttttaaagga ttttattgat gcactcagta
    24181 ccataatatg tgatgaagag tggcgcacgt ctttaaacat caactcagcc acaaaaaaga
    24241 tatttaataa ccttgacaac ttatcatata ttcagaggac ttcttttcgg ggtaacgaca
    24301 cactatacaa tgaaaaggtt cagtttaaac tcacttatcc cgtcaagaat ggaagacaca
    24361 aagaaaatat tgaatttcag gtagtaataa atttaagccc tatttattta gataattttc
    24421 gccatgatgg agaaattaat attttttgcg ctcccaaccc aaagcctgtc actatggggc
    24481 gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt tctgaatgat tttattgaac
    24541 aatttccaat gatcaacctt ggcgttccca tcaaaagagc gcatacacca catattgaac
    24601 ccctgcctcc ggatcaccat accgctgcag attacttacg ccagtttgat ttgcttgtcc
    24661 taaattttat ctctcgcggt aattttgtca tactcccccg attatggagt aactctgagg
    24721 ttcacagatg gttcgtcaat aaagatccta acttgatcac cgccattctc gacataacag
    24781 acggcgaatt aaaagaggat ttattgcaaa gcctgatgga ttcattaggt tctaacaaac
    24841 atgtactacc cgaagtctgc atctgctttt tatccctttt agcagaacaa gaatctcctc
    24901 attttcagga ctttttttta tttttcgcca atatgttatt gcactatcac caatttatga
    24961 atcccaatga aagtgatctg aatgacgtgt tgatgccagc atcattaagt gatgataaaa
    25021 ttatcaaaca tatggcgcgc aggaccctca aactgtttgt aaaaaatgaa acgccgccaa
    25081 aagttactca cgaagacctg gtgaaaaaca ggcctcgctc ccctgtcaga ccacctatac
    25141 ccgcaaccgt caaaacgcca gacctccctg aacgtcatta aaccgtgatg ttaccgactc
    25201 tctgacgcgg aacagaatca gcgtcagaga aacggaaaac gcgatccaga tcacaaatgc
    25261 attgtattca catcattaac cgttttaaga tcatttcatc actttttcgc aactcacccg
    25321 ataatctgtt atgacaacaa acactgtttc ccgcaaagtg gcgtggctac gggtcgttac
    25381 gctggcagtc gccgccttca tcttcaacac caccgaattt gtccctgttg gcctgctctc
    25441 tgacattgcg caaagttttc acatgcaaac cgctcaggtc ggcatcatgt tgaccattta
    25501 cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta atgaccagtc aggttgaacg
    25561 gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc agccacgtac tgtcgttttt
    25621 atcgtggagc tttaccgttc tggtgatcag tcgcattggt gtggcttttg cacatgcgat
    25681 tttctggtcg attacggcgt ctctggcgat ccgtatggct ccggccggga agcgagcaca
    25741 ggcattgagt ttaattgcca ccggtacagc actggcgatg gtcttaggtt tacctctcgg
    25801 gcgcattgtg ggccagtatt tcggttggcg aatgaccttc ttcgcgattg gtattggggc
    25861 gcttatcacc cttttgtgcc tgattaagtt acttccctta ctgcccagtg agcattccgg
    25921 ttcactgaaa agcctcccgc tattgttccg ccgcccggca ttgatgagca tttatttgtt
    25981 aactgtggtg gttgtcaccg cccattacac ggcatacagc tatatcgagc cttttgtaca
    26041 aaacattgcg ggattcagcg ccaactttgc cacggcatta ctgttattac tcggtggtgc
    26101 gggcattatt ggcagcgtga ttttcggtaa actgggtaat cagtatgcgt ctgcgttggt
    26161 gagtacggcg attgcgctgt tgctggtgtg cctggcattg ctgttacctg cggcgaacag
    26221 tgaaatacac ctcggggtgc tgagtatttt ctgggggatc gcgatgatga tcatcgggct
    26281 tggtatgcag gttaaagtgc tggcgctggc accagatgct accgacgtcg cgatggcgct
    26341 attctccggc atatttaata ttggaatcgg ggcgggtgcg ttggtaggta atcaggtgag
    26401 tttgcactgg tcaatgtcga tgattggcta tgtgggcgcg gtgcctgctt ttgccgcgtt
    26461 aatttggtca atcattatat ttcgccgctg gccagtgaca ctcgaagaac agacgcaata
    26521 gttgaaaggc ccattcgggc cttttttaat ggtacgtttt aatgatttcc aggatgccgt
    26581 taataataaa ctgcacaccc atacatacca gcaggaatcc catcagacgg gagatcgctt
    26641 caatgccacc cttgcccacc agccgcataa ttgcgccgga gctgcgtagg cttccccaca
    26701 aaataaccgc caccaggaaa aagatcagcg gcggcgcaac catcagtacc caatcagcga
    26761 aggttgaact ctgacgcact gtggacgccg agctaataat catcgctatg gttcccggac
    26821 cggcagtact tggcattgcc agcggcacaa aggcaatatt ggcactgggt tcatcttcca
    26881 gctcttccga cttgcttttc gcctccggtg aatcaatcgc tttctgttgc ggaaagagca
    26941 tccgaaaacc gataaacgcg acgattaagc cgcctgcaat tcgcagaccg ggaatcgaaa
    27001 tgccaaatgt atccatcacc agttgcccgg cgtaatacgc caccatcatg atggcaaata
    27061 cgtacaccga ggccatcaac gactgacgat tacgttcagc actgttcatg ttgcctgcca
    27121 gaccaagaaa tagcgcgaca gttgttaatg ggttagctaa cggcagcaac accaccagcc
    27181 ccaggccaat tgctttaaac aaatctaaca ttggtggttg ttatcctgta tgcctcaatt
    27241 aaggcaattg tgttgttcga aatgaaaagg tctgggttcg ctgagagtcg actccagacc
    27301 tgtggctggt agctatccga gtgtgtcgat tttattgaag agacaactta atgtgcatac
    27361 cacaagctgc agcataacgt tgtaacgttg taaggctggc acttgataca ttcctctcaa
    27421 gacggctgac agctggttgg ctaactccca tgcgagctgc aatctcggtg ctggttaatc
    27481 cggctctctt cttcatttca atgaggactg cacggagttc ttcttcctgc gtggcttcat
    27541 cgtaagcctt tttaacgtca ggattggcaa gtgcttttgc tttgacttca gaaaacttga
    27601 tacctttaac agccatcttt cagttcctcc catcttgacg tcgccagagc aatttcggcc
    27661 ttcggagtct taggggtctt cttaataaac gttcttaaaa tatagatctt tcgaccttta
    27721 gcgtaagcaa aaaatgttct tgaaatgtct tttcttccta cgcgcaactc aaacaaacca
    27781 tcctggataa tgcctgtatc tggatagcgg agttccggcc ctttcgcttc cagcttttct
    27841 agtcccttaa gtacctttgc ttgcataata ggttcgaggt cataaatttc gctttccgct
    27901 tcggggtgaa atagtagctc atacatagct tccccctgtt tatacttgaa tataacttat
    27961 cagttataat aacgcaaaga ttatattttc cgttcccata cagattagta tcattgcctt
    28021 gcatcgcatt gaccaaaact tgaaccgatt tagcaaaacg tggcatcggt cattcattca
    28081 tttgacttat acttgcctgg gcaatattat cccctgcaac taattacttg ccagggcaac
    28141 taatgtgaaa agtaccagcg atctgttcaa tgaaattatt ccattgggtc gcttaatcca
    28201 tatggttaat cagaagaaag atcgtctgct taacgagtat ctgtctccgc tggatattac
    28261 tgcggcacag tttaaggtgc tctgctctat ccgctgcgcg gcgtgtatta ctccggttga
    28321 actgaaaaaa gtgttgtcgg tcgacctggg agcactgacc cgtatgctgg atcgcctggt
    28381 ctgtaaaggc tgggttgaac ggttgccgaa cccgaatgat aagcgcggcg tactggtaaa
    28441 acttaccacc agcggcgcgg caatatgcga acaatgccat caattagttg gccaggacct
    28501 gcatcaagaa ttaacaaaaa acctgacggc ggacgaagtg gcaacacttg agcatttgct
    28561 taagaaagtc ctgccgtaaa caaaaaagag gtatgacgat gtccagacgc aatactgacg
    28621 ctattaccat tcatagcatt ttggactgga tcgaggacaa cctggaatcg ccactgtcac
    28681 tggagaaagt gtcagagcgt tcgggttact ccaaatggca cctgcaacgg atgtttaaaa
    28741 aagaaaccgg tcattcatta ggccaataca tccgcagccg taagatgacg gaaatcgcgc
    28801 aaaagctgaa ggaaagtaac gagccgatac tctatctggc agaacgatat ggcttcgagt
    28861 cgcaacaaac tctgacccga accttcaaaa attactttga tgttccgccg cataaatacc
    28921 ggatgaccaa tatgcagggc gaatcgcgct ttttacatcc attaaatcat tacaatagct
    28981 agttgataac gtgacaacgt cactgaggca atcatgaaac cactttcatc cgcaatagca
    29041 gctgcgctta ttctcttttc cgcgcagggc gttgcggaac aaaccacgca gccagttgtt
    29101 acttcttgtg ccaatgtagt ggttgttccc ccatcacagg aacaaccacc gtttgattta
    29161 aatcacatgg gtactggcag tgataagtcg gatgcgctcg gcgtgcccta ttataatcaa
    29221 cacgctatgt agtttgttct ggccccgaca tctcggggct tattaacttc ccacctttac
    29281 cgctttacgc caccgcaagc caaatacatt gatatacagc ccggtcataa tgagcaccgc
    29341 acctaaaaat tgcagacccg ttaagcgttc atccaacaat agtgccgcac ttgccagtcc
    29401 gactacgggc accagtaacg ataacggtgc aacccgccag gtttcatagc gtcccagtaa
    29461 cgtcccccag atcccataac caacaattgt cgccacaaac gccagataca tcagcgacaa
    29521 gatggtggtc atatcaatag taaccagact gtgaatcatg gatgcggaac catcgagaat
    29581 cagcgaggca acaaagaagg gaatgattgg gattaaagcg ctccagatta ccagcgacat
    29641 caccgccgga cgcgttgagt gcgacatgat ctttttattg aagatgttgc cacacgccca
    29701 actaaatgct gcagccaggg tcaacataaa gccgagcatc gccacatgct gaccgttcag
    29761 actatcttcg attaacacca gtacgccaaa aatcgctaag gcgatccccg ccaattgttt
    29821 gccatgcagt cgctccccga aagtaaacgc gccaagcacg atagtaaaaa acgcctgtgc
    29881 ctgtaacacc agcgaagcca gtccagcagg cataccaaag ttaatggcgc agaacagaaa
    29941 ggcaaactgc gcaaaactga tggttaatcc ataccccaag agcaaattca gtggtacttt
    30001 cggtcgtgca acaaaaaaga tggccggaaa agcgaccagc ataaagcgca aaccggccag
    30061 catcagcggt ggcatgttat gaagccccac tttgatgacc acaaaattta gcccccatac
    30121 gaccactacc agtagcgcca acacaccatc ttttcgcgac attctaccgc ctctgaattt
    30181 catcttttgt aagcaatcaa cttagctgaa tttacttttc tttaacagtt gattcgttag
    30241 tcgccggtta cgacggcatt aatgcgcaaa taagtcgcta tacttcggat ttttgccatg
    30301 ctatttcttt acatctctaa aacaaaacat aacgaaacgc actgccggac agacaaatga
    30361 acttatccct acgacgctct accagcgccc ttcttgcctc gtcgttgtta ttaaccatcg
    30421 gacgcggcgc tacgctgcca tttatgacca tttacttgag tcgccagtac agcctgagtg
    30481 tcgatctaat cggttatgcg atgacaattg cgctcactat tggcgtcgtt tttagcctcg
    30541 gttttggtat cctggcggat aagttcgaca agaaacgcta tatgttactg gcaattaccg
    30601 ccttcgccag cggttttatt gccattcctt tagtgaataa cgtgacgctg gttgtgctct
    30661 tttttgccct cattaactgc gcctattctg tttttgctac cgtgctgaaa gcctggtttg
    30721 ccgacaatct ttcgtccacc agcaaaacga aaatcttctc aatcaactac accatgctaa
    30781 acattggctg gaccatcggt ccgccgctcg gcacgctgtt ggtgatgcag agcatcaatc
    30841 tgcccttctg gctggcagct atctgttccg cgtttcccat gcttttcatt caaatttggg
    30901 taaagcgcag cgagaaaatc atcgccacgg aaacaggcag tgtctggtcg ccgaaagttt
    30961 tattacaaga taaagcactg ttgtggttta cctgctctgg ttttctggct tcttttgtaa
    31021 gcggcgcatt tgcttcatgc atttcacaat atgtgatggt gattgctgat ggggattttg
    31081 ccgaaaaggt ggtcgcggtt gttcttccgg tgaatgctgc catggtggtt acgttgcaat
    31141 attccgtggg ccgccgactt aacccggcta acatccgcgc gctgatgaca gcaggcaccc
    31201 tctgtttcgt catcggtctg gtcggtttta ttttttccgg caacagcctg ctattgtggg
    31261 gtatgtcagc tgcggtattt actgtcggtg aaatcattta tgcgccgggc gagtatatgt
    31321 tgattgacca tattgcgccg ccaggaatga aagccagcta tttttccgcc cagtctttag
    31381 gctggcttgg tgccgcgatt aacccattag tgagtggcgt agtgctaacc agcctgccgc
    31441 cttcctcgct gtttgtcatc ttagcgttgg tgatcattgc tgcgtgggtg ctgatgttaa
    31501 aagggattcg agcaagaccg tgggggcagc ccgcgctttg ttgatttaag tcgaacacaa
    31561 taaaaattta attcagcctt cgtttaggtt acctctgcta atatctttct cattgagatg
    31621 aaaattaagg taagcgagga aacacaccac accataaacg gaggcaaata atgctgggta
    31681 atatgaatgt ttttatggcc gtactgggaa taattttatt ttctggtttt ctggccgcgt
    31741 atttcagcca caaatgggat gactaatgaa cggagataat ccctcaccta accggcccct
    31801 tgttacagtt gtgtacaagg ggcctgattt ttatgacggc gaaaaaaaac cgccagtaaa
    31861 ccggcggtga atgcttgcat ggatagattt gtgttttgct tttacgctaa caggcatttt
    31921 cctgcactga taacggatcg ttgacacagt agcatcagtt ttctcaatga atgttaaacg
    31981 gagcttaaac tcggttaatc acattttgtt cgtcaataaa catgcagcga tttcttccgg
    32041 tttgcttacc ctcatacatt gcccggtctg ctcttccaat gaccacatcc agaggctctt
    32101 caggaaatgc gcgactgaca cctgctgtca cggtaatgtt gatatgccct tcagaatgtg
    32161 tgatggcatg gttatcgact aactggcaaa ttctgacacc tgcacgacat gcttcttcat
    32221 cattagccgc tttgacaata atgataaatt cttcgccccc gtagcgataa accgtttcgt
    32281 aatcacgcgt ccaactggct aagtaagttg ccagggtgcg taatactaca tcgccgatta
    32341 aatgcccgta ggtatcatta accaatttaa atcggtcaat atccaacaac attaaataaa
    32401 gattcagagg ctcagtgttg cgtaactgat gatcaaagga ttcatcaaga acccgacgcc
    32461 ccggcaatcc cgtcaaaaca tccatattgc tacggatcgt cagcaaataa attttgtaat
    32521 cggttaatgc cgcagtaaaa gaaagcaatc cctcctgaaa ggcgtcgaaa tgcgcgtcct
    32581 gccagtgatt ttcaacaata gccagcatta attcccgacc acagttatgc atatgttgat
    32641 gggcagaatc cattagccga acgtaaggta attcatcgtt atcgagtggc cccagatgat
    32701 caatccaccg accaaactgg cacagtccat aagaatggtt atccgttatt tctggcttac
    32761 tggcatctct cgcgaccacg ctgtgaaaca tactcaccag ccactggtag tgggcatcga
    32821 tagccttatt gagatttaac aagatggcat caatttccgt tgtcttcttg atcattgcca
    32881 ctcctttttc acagttcctt gtgcgcgcta ttctaacgag agaaaagcaa aattacgtca
    32941 atattttcat agaaatccga agttatgagt catctctgag ataacattgt gatttaaaac
    33001 aaaatcagcg gataaaaaag tgtttaattc tgtaaatcac ctctgcatta ccgtaaataa
    33061 aaggatgaca aatagcataa cccaataccc taatggccca gtagttcagg ccatcaggct
    33121 aatttatttt tatttctgca aatgagtgac ccgaacgacg gccggcgcgc ttttcttatc
    33181 cagactgccg ctaatgttga tcatctggtc cggctgaact tctcgtccat caaagacggc
    33241 cgcaggaata acgacattaa tttcaccgct cttatcgcga aaaacgtaac ggtcctctac
    33301 tttgtgagaa atcaaattac cgcgtagtga aaccgaagcg ccatcgtgca tgttttttgc
    33361 gaaatcaacg gtcatttttt ttgcatcatc ggttccgcga tagccatctt ctattgcatg
    33421 aggcggcggt ggcgctgcat cctgttttaa accgccctgg tcatctgcca acgcataagg
    33481 catgacaaga aaacttgcta atacaatggc ctgaaatttc atactaactc cttaattgcg
    33541 tttggtttga cttattaagt ctggttgcta tttttataat tgccaaataa gaatactgcc
    33601 aattgttata aggcatttaa aatcagccca ctagccgtca aatattcaga gaatttaact
    33661 cactaaagtt aagaagattg aagagtctta aacatatttt cagaataatc ggatttatat
    33721 gtttgaaaat tattatattg gacgagcata cagaaaaagc aaatcacctt tacatataat
    33781 agcgtggaca aaaaacagtg aacattaata aagataaaat tgtacaactc gcagataccg
    33841 acactattga aaacctgaca tccgcgttga gtcaaagact tatcgcggat caattacgct
    33901 taactaccgc cgaatcatgc accggcggta agttggctag cgccctgtgt gcagctgaag
    33961 atacacccaa attttacggt gcaggctttg ttactttcac cgatcaggca aagatgaaaa
    34021 tcctcagcgt aagccagcaa tctcttgaac gatattctgc ggtgagtgag aaagtggcag
    34081 cagaaatggc aacaggtgcc atagagcgtg cggatgctga tgtcagtatt gccattaccg
    34141 gctacggcgg accggagggc ggtgaagatg gtacgccagc gggtaccgtc tggttcgcgt
    34201 ggcatattaa aggccagacc tacactgcgg ttatgcattt tgctggcgac tgcgaaacgg
    34261 tattagcttt agcggtgagg tttgccctcg tccagctgct gcaattactg ctataaccag
    34321 gctggcctgg cgatatctcc aggccagcca ttggtggtgt ttatatgttc aagccacgat
    34381 gttgcagcat cggcataatc tgaggagcct taccgcgcca ttgtcgatac aggcgttcca
    34441 gatcttcgct gttacctctg gaaaggatcg cctcgcgaaa acgctgccca ttttcacgcg
    34501 ttaatccgcc ctgctcaaca aaccactggt aaccatcatc ggccaacatt tgcgtccaca
    34561 gataagcgta ataacctgca gcatatccgc caccaaaaat atgggcgaaa taactgctgc
    34621 gatagcgtgg cggtatagca ggaagatcca tattttccgc caccagcgcc cgcaattcaa
    34681 aatcatcgac atcctgcatt gcttcgtttt cttccaggca atgccagcgc atatcgagaa
    34741 gtgcggcgct aagcagttcg ctcatctcat accctttgtt gaacaggctg gcattacgca
    34801 ttttctgttg cagttcgtca ggcattgctg ccccgctctg ataatgccgg gcgtagcgag
    34861 cgaatacctg cggatgcgtt gcccagtgtt cgttgatttg cgacggaaat tcgacaaaat
    34921 cacgcggcgt gttggtgccg gaaagcgtgg cataacgctg gcgggcaaaa aggccgtgca
    34981 gcgtatgacc aaattcatgg aataaggtta ttacatcatc ccagagtaac aacgcaggct
    35041 caccggcagc gggtttctga taattgcaga cgttataaat taccggatgc gtttcattaa
    35101 gcgttgattg ctcaacaaaa ttgcccatcc atgcaccgcc gctttttgaa tcacgggcga
    35161 agaaatcacc gtaaaataac gccagtccca cgccattatg atcaaaaatt tcccacacac
    35221 gaacgtcagg atggtagaca ggaatatcaa aacgttcgac aaacttaata ccgaagagct
    35281 gattcgcggt ccagaataca ccttcattta acaccgtgtt taattcaaaa tatggcttga
    35341 gctgcgcctc atcaagatca aatttctccc gccgtacctg ttccgcataa aatgcccagt
    35401 cccacggctg cgcgctaaac ccgccctgct gcttatcgat aaccgcctgt atggaggcta
    35461 attcatcgct cgcacgttga cgcgccgctg gaacaatttc ccgcataaag ttgagtgctg
    35521 cttctggcgt ttttgccatc tgatcggcga ttttccatgc ggcataatga ggaaaaccaa
    35581 gtagtgttgc ctgttgtgca cggttctcca ccagacgttg aatgatagcg cgggtatcat
    35641 tgccatcatt tttttccgct cgcgtccagc ccgcaataaa cagtttttca cgcgtcgcac
    35701 gatcgcgcat ttcggcaagc gccggttgtt gggtggtatt cagcagcgga atcagccatt
    35761 tgttatccag acctttctcg cgagccgcct ctgccgccag cgcaatctct tgctcactca
    35821 ttcctgccag ctgcgcgata tcgttcacaa ccagaccgcc ggatttattt gctgccagta
    35881 accgctggtt aaactgactg gtcagtgtcg cagcttctgt attcagtact tttaattttg
    35941 ctttatcagc ttgcgcaagt ttggctccgg caaggacaaa acgttgatga atcacctcca
    36001 ccaggcggat ggattcacta tcaagcccca ggaattcacg gcgctgccag acagcatcta
    36061 cccgcgcgaa taattcaccg ttcagataga tatcattagc cagttccgcc agttcagcgg
    36121 aaaactgttc atcaagacgc tgtaattcat cattggtatg cgccgcagtc atcgcaaaaa
    36181 agacgctggt aacgcgggta agtaattctc cgctttgttc cagtgccaga atagtattgt
    36241 tgaaatcagg cgtttgcggg ttaagcgcga tggcagcaat ttctgtccgc ttttgctgca
    36301 ttccctcatc gaatgccggg cgatagtgat gattggcaat ttgatcaaaa tgaggagcca
    36361 gatacggcag tgtgctttgc acaaggaaag gattcattgt tgtcattttc ttctcctgaa
    36421 cgcgaggtgt tccatagcgt aggcttactg ataacggagt gcaatcttgc aatccagtat
    36481 tacccgctct taagcatccc gtgctatgtt attgacacac aaaagcgttg aggaacagtg
    36541 agatgatcgt tttagtaact ggagcaacgg caggttttgg tgaatgcatt actcgtcgtt
    36601 ttattcaaca agggcataaa gttatcgcca ctggccgtcg tcaggaacgg ttgcaggagt
    36661 taaaagacga actgggagat aatctgtata tcgcccaact ggacgttcgc aaccgcgccg
    36721 ctattgaaga gatgctggca tcgctccctg ccgagtggag caatattgat atcctggtaa
    36781 ataatgccgg cctggcgttg ggcatggagc ctgcgcataa agccagcgtt gaagactggg
    36841 aaacgatgat tgataccaac aataaaggcc tggtatatat gacgcgcgcc gtcttaccgg
    36901 gtatggttga acgtaatcat ggtcatatta ttaacattgg ctcaacggca ggtagctggc
    36961 cgtatgccgg tggtaacgtt tacggtgcga cgaaagcgtt tgttcgtcag tttagcctga
    37021 atctgcgtac ggatctgcat ggtacggcgg tgcgcgtcac cgacatcgag ccgggtctgg
    37081 tgggtggcac cgagttttcc aatgtccgct ttaaaggcga tgacggtaaa gtggaaaaaa
    37141 cctatcaaaa taccgttgca ttgacgccag aagatgtcag cgaagccgtc tggtgggtgt
    37201 caacgctgcc tgctcacgtc aatatcaata ccctggaaat gatgccggtt acccaaagct
    37261 atgccggact gaatgtccac cgtcagtaat ttttataccc ggcgtaactg ccgggttatt
    37321 gcttgtcaca aaaaagtggt agactcatgc agttaactca ctcacaagca agaacgaatg
    37381 accgtcgaaa cgcaacttaa tcccacacag cctgtcaatc agcagattta tcgtattctt
    37441 cgtcgcgaca ttgtccattg cctgattgca ccaggcacac cgttgtcgga aaaagaagtt
    37501 tctgttcgtt tcaatgtgtc acgccagccg gttcgtgaag cctttattaa actggcggaa
    37561 aacggcctga ttcaaattcg tccgcagcgt ggcagctacg tcaacaaaat ttccatggcc
    37621 caggtgcgca acggcagttt tatccgtcag gccattgagt gcgcggtggc gcgtcgggcg
    37681 gcgagcatga ttaccgaaag ccagtgctac caactggaac aaaatcttca ccagcaacgc
    37741 attgccattg agcgaaagca actggatgat ttttttgaac ttgatgacaa cttccatcaa
    37801 ctcctgacgc agattgccga ctgtcaactg gcgtgggata ccattgagaa cctgaaagcg
    37861 accgttgatc gcgtgcgcta tatgagtttc gaccacgtct ctccaccaga aatgctatta
    37921 cgccagcatc ttgatatttt ctctgccctg caaaaacgtg atggcgatgc ggtagaacgt
    37981 gcaatgacgc aacatttgca ggaaatcagc gaatctgtgc gccagatccg ccaggaaaat
    38041 agcgactggt ttagcgaaga gtaattcatt tcctctcatc ccattcgggg tgagagtctt
    38101 ttcccccgcc ttatggctca tgcatgcatc aaaaaagatg tgagcttgat caaaaacaaa
    38161 aaatatttca ctcgacagga gtatttatat tgcgcccgtt acgtgggctt cgactgtaaa
    38221 tcagaaagga gaaaacacct atgacgacct acgatcgtaa ccgtaacgca atcaccactg
    38281 gcagccgtgt tatggttagc ggcaccggtc acactggcaa gatcctgtcg attgatactg
    38341 aaggtctgac cgctgagcaa atccgccgcg gaaaaaccgt agttgttgaa ggttgtgaag
    38401 agaaactggc accactggac ctgattcgtc tcggcatgaa ctaagcgtgt gaatgccgcc
    38461 gatggcggca ttgctttttt tacttcacgg aatattttgc cacggtcgct ttcgcgccat
    38521 gcgctaataa agacaagtac gcttccgtca cttttgcagt aaacaagcta ttgtctggca
    38581 aatcatcacc aaagattgcc ttaatcgcca gcaatgactg gacgcgcgct ttcccttcgg
    38641 cactactttg tacagccttc tgaataacag gtaacagtgg gtcactgatt tctatcggat
    38701 tttcctgttc atcaacacca ccgacataac gcatccaacc cgcgacgccc agcgccagca
    38761 gatcgaactt gctgtcatgc gccagatgcc agcgaacaga atccaacatc cgctgtggca
    38821 atttctggct gccatccatc gcaatctgcc aggttcgatg acgtagcgcc gggttgctat
    38881 agcgtgcaat taatcggtta gcgtaatctt gcaaatcaac gccctgcact ttcagcgtcg
    38941 gcgcttgttc ctgcaacatc aagccatacg ccgcatgacg ataatgttca tcttccatac
    39001 agtcattaat gtgctgatat ccagcaagat aacccagata cgccaggaat gaatgactgc
    39061 cgttgagcat gcgcaacttc atctcttcat aaggcagtac atcgctaacc agttctgctc
    39121 ccgctttttc ccattccgga cgtccggcaa caaaattatc ttctattacc cactggcgga
    39181 aaggttcaca ggcaacgcca gcaggatcgc gcacaccggt aagttgttcg attttcgcaa
    39241 gagtatcctc tgtcactgcg ggcacaatac ggtccaccat tgttgatggg aaagtcacgt
    39301 tatcttcgat ccattgtgcc agttttacat caacggcttg cgcgtaggaa gtgacaacgt
    39361 cacgcataac atgaccgttt tctggcatgt tgtcacatga cataacggta aatgcgggaa
    39421 gtcctgccgc tttacggcga gccagcgcct caacaatcac ccctgttgct gttttcggct
    39481 ggtggggatt ttgcacgtcg gcagcgacca tcgggtgatc gagcataaac tgtccggtcg
    39541 ccggagagtg gaaataccct ttttcggtga ttgtcagaga gacaatcgca atttgcggtt
    39601 cacacatcgc agccagcacg gtttctaagc catctatttg tacgtgcaag gcttttttaa
    39661 cgacgccaac gacgcgagcc gtccacgcgt cggccgacat ttccgcaacg gtataaagat
    39721 tatcttgctg ttgtaaatcg gcaatttgct gttcgccgcc gattaagttg acctcataat
    39781 atccccagtc actgaaatgt tccgtagcaa gaatatcggc atacacgccc tgatgcgcac
    39841 ggtgaaatgc accaaagcct aaatgaacaa ttcttggggc caggttatta cgatcataaa
    39901 cagggagtgt cgcttttgct gataacaaat tatttcccat aacaattcct taaatataaa
    39961 tatggcaagc tatatgtttt gttatatgaa taaaaatccc ctctccggta agagaaggga
    40021 ttaagggttt acagacttct ggaaggttgc gcagctctta caacacgcgg ttgatcttcc
    40081 gcagcgtctt ccagcgcact taaatcacgg tctttcacct ctggcatttt cagcgcagag
    40141 attaaaccaa tcactgaata tgccatgatc ataatggcga tcggatacca ggattccgtc
    40201 atggtgcaga aaatacccgc caggatagga ccaaaaccgg aagcgataag accaccaatt
    40261 tctttagaaa tagccatccg ggtaaagcgg tttttacagc cgaacatttc tgccatggta
    40321 atgttttcca gagcaaataa tcccagcacc gcacagttat gaatcacaat cagtgcaacc
    40381 ataatggtgc tcggggcata gcttttatct acaatgatag aaagcattgg ccatgccagc
    40441 acaatcgcgg aggtattcat aataatatac gggatccggc gaccaatttt atcggataac
    40501 caaccaagga acggaatggt cataaagccg agaatcgaac tgatcatcaa tgcatctgtt
    40561 ggaattgctt tgttaaacaa taacgtctgc actaaatagc ctgcaaggaa agtctgaatt
    40621 aacccggagt tacccgcctg accaaaacgc agccctgttg ccagccagaa ggatttgctc
    40681 tggaacatgc taccagcagg tgcaggtttt gctgtcggtt ggttactgtc gttaaccttc
    40741 tcaaagaccg ggctttcttt cagattcata cgcaaccaga tagcaaagac catcacgaca
    40801 acgctcgcca ggaacggtat acgccatccc cacgccagca gttcctcttt actgagaatg
    40861 aagaacataa aggcccagat tgccgttgcg ctcaaggttc cgcagttagt tcccatagcc
    40921 acaaatgagg agataattcc gcgcttacct tttggcgcat attccgccag catcgtaccg
    40981 gcaccggaaa tttccgcacc tgcacccaac ccctgaataa tacgcaacgt caccagcaag
    41041 atgggtgcaa aaacaccaat ctgtgcatag gtcggtaaca caccaattaa ggtggtacag
    41101 atccccatca tggtgatggt aataaagagc acttttttac gccctattct gtcgcccatt
    41161 ttgccgaaaa taaatgctcc gacaatacgc gccacataac ctgcaccgta ggttcccatt
    41221 gccagaatta acgccattgc cgttgatgat tcaggaaaaa atatttcatg aaacactaac
    41281 gctgcgccga gcgaatataa ctggaaatcc ataattcaca ggtgtttttt cccatcctgt
    41341 ggtttccttg gcgttttcta ggttttttca gatagttgca tttttttaaa agcatcctaa
    41401 gttcgatctc agtgtctatc tggggcctat ttctgtccca tatatgcccc aaaaaactcc
    41461 ccaacagata agtagttttt tcatggattt acgcgtaaaa tcaataacgg ctggaaatca
    41521 ttcaatactc gcactatcga aaaatttacc agccaaccgc agcacgttct tgcataaggc
    41581 gtgtctgcgg tttttcaact attcagatac atcactccca tcacattcat tcctccgcat
    41641 caaaggcata taggctatat caccttgata tttttcttct tcagataaaa attgttatct
    41701 atgtatactt ttaaacccaa tccgtgtaga gtctctgcat aagatagttt gcagttgcca
    41761 cttcagattg cgccataaac cgcctgattt ttgccgccac ctgttagcat tcctgtatac
    41821 ctgaaacgac aatgtttatc tacgaacttt aagaacaccc aagataaaaa ttgtcaacta
    41881 tatcatatat aacacattac taattcgagg ctatatgaac agcatactga taatcacttc
    41941 tctccttatc atattcagca tttttagtca tgccctaata aaattaggga ttggcatatc
    42001 caataaccca gacaaaaccg atgtataagt caacatatcc tgaatcagac atacaatatc
    42061 gcaatgaaaa tcaataatat tttaaggaat atcttcatga aatcaaaaga caccctaaag
    42121 tggttccctg cgcagcttcc tgaagtaaga attatcctag gggatgctgt agtggaagta
    42181 gcaaaacagg gaagacctat caataccaga acattgcttg attacattga aggaaacata
    42241 aagaaaaaat catggctgga taacaaagaa ttattacaaa cagcgatatc agttcttaaa
    42301 gacaaccaaa atttaaatgg taaaatgtaa tataacaaac ttactttttt atcatttttc
    42361 cactttaaca acattttgct ccacttttcc acgaccaaac aaattaaaat ctggttaaaa
    42421 taacacgcaa cactattctt cttccttgag tctgcccgga actcgaaaaa caaaccgagt
    42481 taaagccatt tttcacaaaa tcgattttgg gtctcaccaa aattacgggg ttgcatacgc
    42541 attcgtttat tttcgaacgt gtacatacaa atatgcacaa aaataatcaa aattattttc
    42601 tgagatgcat tatgatatga acaccaattt cgtatagagt ctcactatgt ctcgaatttt
    42661 tgcttactgt cggatatcaa cgctggatca gaccaccgaa aatcaacgcc gggaaatcga
    42721 aagtgcaggt tttaaaatca aacctcagca aataatcgaa gaacacatta gcggctcagc
    42781 agcaaccagt gagcgtcctg gttttaaccg gttgcttgct cgcctgaaat gtggtgatca
    42841 attgattgtg acaaaactgg atcgccttgg ttgtaatgca atggatatca ggaaaacagt
    42901 ggaacaactg accgaaacag gtatcagagt gcattgctta gcattggggg gcattgacct
    42961 gaccagtcca acaggaaaaa tgatgatgca cgtaatttca gcagtcgctg aatttgaacg
    43021 agacctttta cttgaacgca ctcattccgg gatagtaaga gcccgcggcg cagggaaacg
    43081 ttttggtcga ccacctgtgt taaatgaaga acagaaacag gtggtattcg aacgaattaa
    43141 gtcaggtgta agtataagtg ccattgcccg ggaattcaaa acctcgcggc aaaccatttt
    43201 aagagccaaa gcaaaacttc agacacctga catataaaaa ataatctcgg tgtgagatgc
    43261 tttacgtctt ccaagccccc ttccttgccg taaatggaaa gatacatcta attatagaat
    43321 ttatatgttt taccctacgg cagtgctggc cattcaatat cctgtgcagt tgatgtatca
    43381 acacggttca gcaacacccg atactttttc caggcttcca gcaatgaggt ttcttcctcc
    43441 gttgcgattt ccagatctac agcatcctga agtggcgcta tatgctcact ggctacctgc
    43501 atcaggctgt tttttgtttc ttccgcctcc cggatccgga acagtttttc tgcttccgta
    43561 tccttcaccc agactgtacc gttccacttc tgaaactccc cttccggcga taaccaggta
    43621 acattttccg gtaacggacc gagttcagaa ataaataacg cgtcgccgga agccacgtca
    43681 taaaccattt taccccgatg atcttcaacg agatgccacg atgactcatc actgttgaaa
    43741 acagccacga agccagccgg aatatctggc ggtgcaatat cggtactgtt tgctggcaga
    43801 cctgtatgag gcggaatata tgcatcacct tcaccaataa attcattagt tccggccagc
    43861 agattataaa tttttatggt ccgtggttgt tcactcattc tgaatgccat tatgcaagcc
    43921 tcacaatata gttaaatgcg atgtttttga cggtgttttc cgcgttacca gcagcgttaa
    43981 cggtgatggt gtgtccatgt gaaccaatcg caacggagtg cgtatgagca ccaataccca
    44041 cagtatgtgc gtgtgcacct gcgcttgcag cagtacccga tacagagtgc gtatgagcgc
    44101 ctgcagaaga agtgttaaca ctgtatttag aataatcaga tgctccactt cccggagcac
    44161 cactggaacc acctgaaaca aatgtcatcg aatgggtatg tgcaccggct gaagcagccg
    44221 taccgctaac actatgggta tgcgccccgg tgttattcgt ggatttagtg ccgtaatcaa
    44281 acgacgatgt ggttttcgtc cccaaatccg tactggatgc gctggcgctg tgggtatgcg
    44341 atttaatgcc gtcctgttcc tgagacaata cggcccgacc actggcaggt ttgcccttaa
    44401 tcgtccagcc acgcatatca gggatcacgc ctgacggata agcggctgca agtttcgggt
    44461 aagcagattt gtcaaaagtc tgcccctgca tcagggcata accagacgga acggtatctg
    44521 atggccacgg gattggtgcg ccgactgggt agctttctgg tggaagattt ttcgaggtat
    44581 aaacttctgc ccagtcttcc tcaaaaccat aaccgtctct tgaagaacgg tagaacagac
    44641 caccatttct gtaatgcgcc ttcatctgca aggtccggca acttccgact ccggtataga
    44701 agttaaccag aatatagctg tcgccagagc gggtgacatt gtaagcacct gattcggcat
    44761 tccagggaac gccaccatcc gcatcggcat atgtatccgt tgcccttctg gcaaaagcag
    44821 ccacatgcgc ggcggttaaa gtaatatctc tggaaccatc aaactcaaca ccagaaacca
    44881 gtcttggcgt ttgcagcttt gttgctgtta atgcattacc gttcagactt gcggacagtt
    44941 tggttccaat aaccagttcg ccggttgcgt tataaatagc aaacggtctt aatgtattcc
    45001 agccaccata aacatcacct tgattggtaa gcagcaggta agttttagcg ccatcattac
    45061 gccataatgc accatactcc ccacctatca ttcgaatctg attaccacca cgcgctacaa
    45121 tttcgtctgt ggcaaaaagt tttttgcacg acaaattatc gttaacgatt aacgaatgag
    45181 actcataaaa accacgccca ctcttaaaat caaggataac gtccgccgcg atacattcag
    45241 tcgctggatt tgttgcccca aacttatagg tcgtatcatt aacaacgaga tcagcaccag
    45301 gtgcggatat tgacaggcca tcttcgataa acgcaaaaac agggaaagca gcgccatcaa
    45361 catagaacac agagcgcaaa tcatcgccct tattactcat cattattgag tggatggctc
    45421 gttcattgtt ttgatattgc cagaacattc cataagcata acgccccctg tcagtccagc
    45481 caccaggcat aacaaatccg ttaaactcgc agttattcat cggatcgcct gcggttcgcg
    45541 ttgccgtggt gataatgacc cttgatgcca gttcgcttac tgagccagca gaacgcataa
    45601 caacaacagg gtaatatttt ccagatgttg cacctgcagg agcgttaacc cgcacataac
    45661 gcataccacg cttatcagca aagtctgttt tactgaccgc gttaatgttg ttcaggaagc
    45721 gtcccttatc gggtatatca gcgccgttct ggtctttctg cagacgtttc tctgcattgt
    45781 cataggctga ttttactgcc tttggcgttg ccgccagcgt ttcagacgta ctgttggtcg
    45841 cactgctgag ctgtactatc ccctttttcg tcgtgctcgc atcctcaagc gccacggcgg
    45901 atgcaatatc ctctgcccgt tttgccgctg tctcggcgcg cgttgccgcg gattccgccg
    45961 tacttttgct ctgtgctgcc gccgtcgcac tgccagctgc ctctgtcgcc ttcgtggatg
    46021 ctgtcgtggc gctgcccttc gctgctgacg cctgtctggt cgcctcatct tttgaagcag
    46081 acgccgatga tgccgatgac gccgccgaac tggctgacga tgcggcagcc gtttttgagg
    46141 attctgcgct ggtttccgac gctttcgcgt tcgtttcgga tgtcttcgct gcggaagcag
    46201 aactcgctgc tgcgctggcc tgttcagtgg cttcgccagc cttcgttgtg gctgttgaag
    46261 cggatgatgc ggcgctttct gccgattttc cggcggcggt ggcactggct gaggcctgcc
    46321 cggcacttgt tgacgcggcg ctggcagatg atgcagccgc tgtttttgag cctgccgcag
    46381 ctgaggcgct ctgtcccgct gccgtctcag aagacttagc gttcgtctcg gacgtttttg
    46441 ccgccttcgc ggaatttcct gccgccgttg ccgaggaagt tgcgctactg gcgctcgagg
    46501 atgcgttcgt ttctgatgat ttcgctgcct cttttgaggc cgccgcatcc cgggctgagg
    46561 tggcagcttc tgacgctttc gtggtcgcgg tggatgcaga agtggctgct gattgttgtg
    46621 acgctgcggc attcgtttct gacgttttcg cggcactggc actggtagct gccgcgcttt
    46681 ttgaggactc tgcagcagca gcacttttcg atgcttcact gacctttgtt gatgccgttc
    46741 ctgcgctgga agacgctgac tgagccgacg acgcggcctg tccggctgac gtgctggctg
    46801 cgcgtgctga gcctgcagca tcagtcgcat gggttgccgc ctcacgggct gatgtgccgg
    46861 catcgccggc tgacttcttc gcggctgccg tgttctgtgc caccgcggac gcgttacgcg
    46921 ccacctcttc caccatcagc tcaaaacggc gcagtgcctc tggccgggca tcatcctccg
    46981 tcatggcacc gagaaaatca ttcagcgtac cgggtcggga gtcttcatac acggtgatgg
    47041 tcccggcatg tgacggcggg aatccttcca ccaacagaat aacgctgtac tgaccgtact
    47101 caacgtccat gctgtaacgc ccggcttcat ccggattttc tgaggccacc gtgttcacca
    47161 ccaccgtggt gctgttacgt tttgctttca gctggattgt gcagttctgt accggttttc
    47221 ctgtgccgtc tttcagtaca cctgaaatct ttactgccat attcacccca caaaaaagcc
    47281 cgcctgaacc ggcgggctgt cataacactg tgttacctgg ctaatcagaa tttataaccg
    47341 acacccacga tgaaaccgtc agtgcgccag tcaccactgc cggagccttc ataagcaata
    47401 tcaatggcca cggattcggt cgggttaaac tgcacgccag ccccccacgc cagagacgtg
    47461 ttgctgtggc gaccgtcatc acttccggtc agcacatcgt gcgttttccc cttgttgtca
    47521 gttacgcgga gataatcccc gaagaaagtc gacacacggc tgtaagccac acccgccatc
    47581 gcatacacgc tgaaccattc attcacgcgc acagacggcc ccgccattac gctgaaccag
    47641 cggttacgca cggaatcttc atgccagcgg gtatcgctgt aatgcgtttt ttgctcatct
    47701 tcagcgttgg cataactgaa tgacgtaatc agccccagcg tgtccgtaaa ctcataacgg
    47761 tatttcacgt taatcccgtt cagattatcg ctgccgggag cgttcgtacg ggcattaaga
    47821 taccctgcgc tcagtgtggc ctgctgctca gacgcccatg caggcgcacc ggatacggcc
    47881 agacagatgg ctgtggacaa aatggctgca caaactttac gcataattac ctctcgcttt
    47941 tctgcaataa aaaaggcgcc atttctggcg cccgtatatg ggttataaaa ttcagctgat
    48001 actgatgcct gcggtggctt tcttcatcac cacaaccagc aaatcgctga tacttgctgt
    48061 gggataccag tcatttacca gccatgctga caccgaaaac tccagcgtca tgtggccgtg
    48121 accagctggc atatcaataa caccactgta aatcagcgta ttatccagcg cggtacggtt
    48181 ataaatttca gcaccatttt tcttcactat caggcggcat gaggagtaaa tatcagtatg
    48241 ctctctctca tgtttagcac cgctgaatgc caccgccgga ataacaatct gccggtcaaa
    48301 cggctgatcg tcataaatcc tgacggtaat ggtccctgat ggccaccgct ccggtgcccg
    48361 ggagtcccgc gggaaagcct tacccactgt tttgacaata tcgccttcaa tctggttggc
    48421 tgacagtttc cctttaatct gacagttttc atttatcgta acgttgttga gcgtcccggc
    48481 gttcgcattc acactgccac tgatatctgc atttttagcg gtcagctttc cgtccggtgt
    48541 cagggaaaat gcaggaggat taccgccgct ggtaatggtg ggagccgtca gatatttcag
    48601 gaacacttca ttcatgaata tctgatcacc ctgaccaaca aacatcggtt ttgtgttgcc
    48661 atttgcagga ttaatcatcg caatcctgtc tgccgccagc agcacctgac tctgcatgcc
    48721 gtcgggggtg ttctcaatac cggcaccgat acccgcaata taaaggcgtc cgtcctgcat
    48781 ctgctgcagc ttcacagccc acatgctgtt cagattatta tttgtatcaa cctgaacctt
    48841 ctgtatctgc tggatcgctg cactctggtc ttccagtttc ttattgacgg tctgtgtgat
    48901 ttcattgctg acatccgtaa tggatgtcct gatttcagcc aggtcaggcg caagctgacc
    48961 gttatcaatc tgcgtccaca gctcctgagc cagatgggtt ttccctatct cgcctttgaa
    49021 aaaatccaga tagccggatg cgtcatcact cggctgacca acagcctcca cgaatgccga
    49081 tttgccaacg gtgttcacac tgcgaacgta aaaataataa ttatggcccg gcctgatatt
    49141 gatactggcg gctatccagt acagcgccgt gccaagatag cgtgctgtgg tttcaacctg
    49201 cctgatatcc gcaatccgct tttccgagaa ccagaactca aactgtaccg tcggatcata
    49261 aaccgcaaga tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct caatccgcga
    49321 cggcgctgcc ggtgcggcaa tccggaacga taccgacgcc ggatcgccct gctgccccca
    49381 cgcatttacc gcccggactg tcagcctgta gttccccaga gccagttgtg tgaagcggta
    49441 agtggtttcc gtcgtccggg ccgtgctgac cagccgctca ctgccgtcat ccgctgccac
    49501 ggtcaggcga agcaggaagc tcacgccctt caccaccttc ggcgtgtccc agcgggccag
    49561 cacctggtat tccccgctgt ctgcagtgac ttctgcggtc aggtgctgca ccgctggcgg
    49621 cgtgacaccg ttcaccgtgc cactctgttc gccgtcaaag tgcgccccgt tatccacgat
    49681 gacctctttt tccggcacat gctgcacggc ggtgatggca tacgtgccgt cgtcgttctc
    49741 acggatactc acgcagcgga acagtcgctg gcgcagcctc ggcagcttca gcccccatac
    49801 gctgtattca gcaacaccgt caggaacacg gctcactttc accttcacgc cgtcggtgac
    49861 ggactgaacc tccacgctga ccggattgcc acttccgtca accaggctta tcagcgtggt
    49921 accggaggat ggcagcgtga tttcacggtc aagcgtcagc gtccgggtca ggctgttcac
    49981 cgccagcaca cgaccaccgg tgctgatacc ggcatagtca tcatcacaga tttcaataac
    50041 atcgcccggt acatggcgaa gcccttctgc gccgacgctg aaatccacgg tctgcgtttc
    50101 cagcagctcc gttttaatca gccacagccc ggctcggtgt gcctgccccc ggctggtaca
    50161 gccaaaggca tccatcttcg tgacattacg accgtaacgg gcaatggcct gcgtgtcctc
    50221 cacaagctct gtcgccgtct cccagccgtt atccgggtca atccagttca cctcaacggc
    50281 attatggcgg tccttcaggg cgctgaagct gtagcggaac ggcgcgccat catccggcat
    50341 caccacatta ctgcggttat aggtccacac cttatccgac ggtcggtcct gcacgaacgt
    50401 cagcgtctgc ccgttccata ccggcataca gcgcatcgcc gagcagaaat cactgagcac
    50461 atcccacgcc ttgcgctgtg tggtcagcca ggcattacag gtgatgcgcg gctccgtgct
    50521 gccaaagccg tccggcaccg actggtcgca gtactggccg atgacataca gcgcccattt
    50581 atccacatct gccgcaccaa gacgtttccc catgccgtag cgcgggtggg tcagcatatc
    50641 ccacagacac caggccatgt tgttgctgta tgccggttta aacgttccgt cccagatacc
    50701 gctgtattgc cgcgtctgcg ggttataatt cgacggcacc tgcagaatac gcccgcgcag
    50761 atgataatta cggctcacct gctggctgcc gaactgctcc gaatccacct gtacgccgac
    50821 cagtgccgtg ttcgggtagc actgtttcac atcgatgatt tcggtgtatg acgaccagag
    50881 cgttttgttc tgcagctggt ctgtggtact gtccggcgtc attctgcgca tccggatatt
    50941 aaacgggcgc ggcggcaggt tatccaccac caccgaggcc agatactgcg aggtggtttt
    51001 gcccttaatg gtgatgtctt tttccgtcac ccagccacca ttacgctgta tctgaaccag
    51061 caggcgaact tccgacggat tccggtcccc ctttgaggtg gtttccacca gtgcctgcac
    51121 accgaaggta aagcgcagtc ggtcgatgtt tgccgacgtg atggcgcggg tgatcggcgt
    51181 gtcgtatttc acttccgtac ccaacaccgt ctcggagccg gaggattcaa atccctccgg
    51241 cggagtctgc tcctgctcac cggcccggaa caccaccgtg acgccggaga tattggtatt
    51301 cccctcactg tccagcaccg gcgtactgtt cagcagcacg ctttttaatc cctccaccgg
    51361 accttcaatc ggcccttcgc tgatggcatc gatcacactc agcaactgcg tggtcttcag
    51421 gttgtccttc gcttcgcgcg gagtatgccc cttactgctt cctttaccca ttcctcacgc
    51481 tccataaacg acaaaaccgc ccgcaggcgg tttcacataa aacattttgc atcagcgacc
    51541 aatcaccaca acctgaccac catccccttc atctgctgtg ctgatctcct gagataccac
    51601 ccgcgacccc acacgcattt caccgtacag aacgggcagg acattgccct gggcaaccat
    51661 attatccagt gaagaaaaat aggtgttctg tttgccgtta tccgttgtct gtgtgcgggg
    51721 agttctggct ttcggtgcca gcatctgcgc cacaccaccg agcaccatac tggcaccgag
    51781 agaaaacagc agattactcg ccataattcc tacccccggc atccatatag caaccgccat
    51841 aacagccgcc cccagcacag cctgaaacac accgccactt ttggctcctg ccagacgcgg
    51901 cacgatatgg atcacagcac catttgccag cggttcatta agacgggctg ataattcatt
    51961 ttcgcctgca tcacgcccgg caatgcgcac ctgataccag ccctcattca gtttctggcg
    52021 aaacgacgga agctgcgtgg ccagcgccag gatggcttcg gcccccgttt tcacacgaag
    52081 gtcgatgcgg cggccaaatc gttgtaaatc cccgtaaagg cagatgcgtg ccatgcccgg
    52141 tgacgccaga gggagtgtgt gcgtcgctgc catttgtcgg tgtacctctc tcgtttgctc
    52201 agttgttcag gaatatggtg caacagctcg ccgtcgccgc agtaaatggc ggcatgattc
    52261 ggcaccgatg aaccaaaaca gcacagcagc acatcgcccg gctgcgccgc tgacaacggc
    52321 acctgataca gccctgtggc ctccagatta tccagataga gattctgacc gtgacgccac
    52381 cagtcatcct cacgatgaaa atcaggcatc tcaattcccg ccagatggta agcatcccgg
    52441 aacagcgtgt aacagtccgt caccccgtgc tcaaagcgcc gcccggtaag atgtggcaca
    52501 cagcggaact tgtgaatcgc cccccggcag accagccacc acggcaaatc actctgcacc
    52561 tgcagccgcc ggtcggcctc actcagccag ggcagagcac cggggtgact gtggaccagc
    52621 gccacaatct caccctgcat ttctgcccgc agccagtcct ccggagccat ccggaaatac
    52681 gcctccggct caccggagat attcacgcag ggaaaatatc tttccccttc cggcgtgctt
    52741 accacgaagc cgcacgactc cgctggcgca catcgccggg cgtgcgccag aatcgctgat
    52801 tctgtctctg tcatgggatt tactgcgaaa gtttgttaat ggaaaggaag ccgccaaagt
    52861 tgccgacgtt attgcgaaac ttacaaccgc tcaggcattt gctgcattta tccttcgtga
    52921 tatcggacgt cggctggtca tattcatccg cgacagccgg accgtgataa ccgcactcat
    52981 caccgcgata ggtccaggtg caggtattag ccagcatgat gcggcccgga aaaacggcac
    53041 catccgtttc cgtcggtgtg gagagcacaa aggaagcact gaccgcgctc agttcgctgc
    53101 actgctcgat gcgccagcgg ctgatcacct cctgctccgg atcggcgtcg ctgtttccgt
    53161 tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgccggacc accgttccgc
    53221 cgaccagact ctgcagatct tccgccatct cggtgaccat accgtacagg ttagaaaccg
    53281 tcagcgtggg gcgcgtactg gtgcctttgc cattcagttc aaaaccactc ccctgaatgg
    53341 gatacggctg atactgtcgc ccctgccagg tgaccggctc acctttttcg ttctgctcat
    53401 tacagaaaaa ataacgttct ccaccgacct ctgtcaggtc gatttcccag agcaccacgc
    53461 tggccgactg ctccgcacgg gtgcattcat tcagtgtttc ctgccggata tcctgcatca
    53521 gttcaccacc tgttcaaact ctgcgctgaa ctcaacacgc agcatactga cccgcgacga
    53581 ccattttgcg caggtcacct ttatctgccg ccactcataa ggcggcgtcc acagaaaggc
    53641 tttccagccc ccgtgctctt ccagaaacga ctccagtacc gtggcctcct cacgggggac
    53701 agaaagcgtc acgctgtacg ttttcaggtt ggcattcagc ccggcaggcg ctcgctggga
    53761 atagccatca ccaaagcgca cctttcttac agaaggggcc gaagccacat ccataccggg
    53821 tttcactttc cagcggaagg ttttcatcgt ccacctccgg agaacagacc accatcgcgc
    53881 atctgtgcct ggatttcatc acgggcaccc ttgcgggcca tgtcatacac cgccttcatc
    53941 atctgtggac ctggcagacc attcgtaccg tcgttctgaa tcaccacgtg attgttctga
    54001 ttaaaattaa tgccttcagc ccgccgcatc tgcgccggac ttccggcacc gcccacataa
    54061 ccaccttccg catagccccg catcaggcgg tacaggttgc cgacaccaat ccggctggtt
    54121 gcctccttcg tgaagacaaa ttcaccacgg tgaacaatcc ccgctggctc atatttgccg
    54181 ccggttcccg taaatccccc ggtcgcaaaa tggaatttcg ccgcagcggc ctgaatggct
    54241 gtaccgcctg acgcggatgc gccgccacca acagccccgc caatggcgct gccgatactc
    54301 ccgacaatcc ccaccattgc ctgcttaagc agaatttctg tcatcatgga cagcacggag
    54361 cgggtgaagc tgcgccagtt ctgttcactg ccggtcagca tcgccgccat attctgcgca
    54421 ataccatcaa aggtctgcgt ggctgcactt ttaacctgcg acatactgtc cgtggcactc
    54481 tctttccact cgctccagcc ggacctgagg cctgccatcc agttcccgcg aagcaggtct
    54541 tcagccgccc aggtcttttt ctgctctgac atgacgttat tcagcgccag aggattatcg
    54601 ccatactgtt ccttcaggcg ctgttccgtg gcttcccgtt ctgcctgccg gtcagtcagc
    54661 ccccggcttt tcgcatcaat agcggcccgt tttgcccgtt gctgctgtgc gaatttatcc
    54721 gcctgctgcg ccagcgcgtt caggcgctcc tgatacgtaa ccttgtcgcc aagtgcagcc
    54781 agctggcgtt tgtactccag cgtctcatct ttatgcgcca gcagggattt ctcctgtgca
    54841 gacagctggc gacgttgcgc cgcctcctcc agtaccgcga actgactctc cgccttccac
    54901 aaatcccggc gctgctggct gattttctca tttgctccgg catgcttctc cagcgtccgg
    54961 agttctgcct gaagcgtcag cagggcagca tgagcactgt cttcctgacg atcgcccgca
    55021 gacaccttca cgccggactg tttcggcttt ttcagcgtcg cttcatagtc ctttttcgcc
    55081 gccgccatca gcgtgttgta atctgcctgc agaattttcc cgtccttcag tgccttgttc
    55141 agttcttcct gacgggcggt atatttctcc agcggcgtct gcaggcgttc gtaagccttc
    55201 tgcgcctctt cggtatattt cagccgtgat gcctcagact cggcccagtc ctttgctgcc
    55261 atctctctgg ccttttcaag atcggcctgc aacgtggcgg ctgaaagccc aagttgcgca
    55321 ttcgctctgt cctcccatgc tccccggaga ttggcaagaa atgctgaggt tttaccgcgc
    55381 cggtggctcc ggctctgata ccactgccat tttttgtccg cctcatcaaa agccttttct
    55441 gctttctcca gcattccctg ggcagtgtcc gggcgaccaa tatccagcac cgaatcccac
    55501 atggatttga atgcccgtgc tgtcctgtct gcccaggtct ccagcgtgcc catgttctct
    55561 ttcaggcggc gggtctggtc atcaaaccct ttcgttgcgg cctcgttcgc cgcctgcaat
    55621 gccccggctt catctccgga acgctgcaac tgagcaacat acgcaatctg ctccgccgtc
    55681 acgttatgga actgacgtgc catcgctgtc agtcccgacg tcgggtctgt ggtcagcttc
    55741 ccgaaggctt cagcgacctt gtccacctcc acgccggatg cagaggagaa acgcgccaca
    55801 ctctggctga tcgcctcaaa ctgctcacca ccacgcacac cggcattcac cagcgccgtc
    55861 agtgactcgc tggtctggtt aaacgtcagc cctgccgcct gcccggctct ggacaggacc
    55921 agcatacgat ctgccgtcag tcccgcctga ttgccggaaa ggaccagcgt tttgttgaaa
    55981 tcggacaggg ttgagttgcc ctgataccag gcatacgcca gcgcaccggt cgccaccgcc
    56041 agcgaggtgg cccccaccat cggcagggtg atcgcaccgg caagccccct gaacatgggg
    56101 atcatcccgc cgaaggagtc cttaacctga ccaccctgtt gcagcaggat cagccacggg
    56161 ctttgcccgc ctgcaagctg cgtggccacg tcggtgaact gtgcaggcag catacgcatg
    56221 gcggctttat actgcccgac ggaaatcccc gctttctgtg cagccagcgc ctgtcggctc
    56281 agcgactgtt caacgactgc cgctgttttt ttcgcatcag tttccgtacc ggaaaaatga
    56341 cgcctgactc tggccatctg ctcgtcaaat ctggccgcat ccagacttaa atcaacgacc
    56401 agatcgccta ccggttcagc cataccggac tcctcctgcg atcccttctg atactgtcat
    56461 cagcattacg tcatcctccg tcatgtccgc cacatccggg gaagcgggga taacttcatt
    56521 cccgtccggg ccaaaacgaa cgcctccggc aagccctgcc gctttctgca tcagcacatc
    56581 atcttcaggc tcttcgtcag cctcgcgccg gttcagcaga ctgaaatcca gcggatgcat
    56641 ctccggatcg ctgaaaaaca ggctgagcac ggtgtacgtc agcccggaaa agtgcatatc
    56701 cagcagaaca tcatgaaaat aatgggtact gtaaaagcgg tgccagtcgg catactccgt
    56761 ggatgacatc ccggcaagca tggcgcgcca gtcgggtcgc cccatctcac gcgccagttt
    56821 cagggcaaaa ctcagctcac cgtcgaacac tttcccgcag aaacaggctc tgcaggcccg
    56881 gcgtcctctg cctgttcagg agcatcattc accacaaact catacatacc ggacagccgg
    56941 tacaccacgt tttcagcatg agaaattgcc tctgtgggcc aggtggtaag cacttcctgc
    57001 tcaatctgtt taacggcttc attcatggaa ggcagctttg tcttcttcgg atggttatgc
    57061 cacagggaca tcgccaccag aaacgcgccg gttctgatgg cgtcttccac agtaaacttc
    57121 cggttgctgt ctgactccgc ctgttctgcc tgccgtttca tcagggcgag atgctcaata
    57181 cgctgcaggg ctgacagttc agaaagcgtg acggtcacac cgttatgttc aaatgattcg
    57241 gttttcagga acatcgctga ctctccggat taactggcgg tgacgttgat ttctgcaacc
    57301 gcagcaaact caccattacc ggatacgacc ggaatgttga ccttgcctgc agcaacacct
    57361 ttcacggtga tggtcatacc actgaccgac acggtggctt ttgttttatc cgcagacacc
    57421 gcacggaagc tcttgtcggt tgcgccatcc ggctggaatg ccacggtcag cgtggtgctc
    57481 tgccctttca ctacggaagc actggcgggt gtcaccgtca tgccggttgt cgccgtcacc
    57541 gtgctgcgat cttctgccat cgacggacgt cccacattgg tgaccttcac cgtgcgggta
    57601 atcacttcct tcgccgtcac cgccttaccg atactgctga cccagccacg gaacacatcg
    57661 accgtgccgt tcgggaagcg gattttatag gcacgggtat cgccttcatt aaaccacgcc
    57721 agcagcgcct gctgcccctg ctctccgggc atccacgcca gcgtgaagct ggtatctccg
    57781 gcagatttct gcccctgccc ggtcgcagtc cagtctgcat cttcatcatc gagatagctg
    57841 tcgtcataag actcagcggt cagttcgccg ggcgtcaggt ctttaacttt tgccagacgc
    57901 gaccagtcaa cgtctgaaag cggattcgca taagggtcac cactcccctt ataaacccac
    57961 agggtggtcc cggccccttt caccggcatt acaggatttg gtacaggcat agcgtcctca
    58021 catttcatag gtaatgacat aagtcagatc ggctgaactc cacaggcccg catcatcgtc
    58081 gcgccgatag tcatagccgc tggtcaccat actggtgatc aaatctgaca gtgccgggat
    58141 atcactcatc accggataaa tccgggactc catccacgca tccagctctg aatccggcac
    58201 ctgagcaggc aggaaaactt caatatgcag ctccgcctgc caggtatcgc tgtccagctc
    58261 ttcgcccgtg tattcagcgc cggtgagata aacggcaatt gccggaaaat cttcctcatc
    58321 aaaaacagcg gggcgaccat caaaaagcgt cgccccggtg tcatgcttct ccagtgcatc
    58381 cagtacggct gctcggagtt cagtatgttt catcgcttta ttaccatcct cagttgatgc
    58441 tgcagcgcat agcccagctc tttcggaaga cgctcacgcc gtatccgctc aatattctgt
    58501 ttaaacgccg tggtcagcgg caccgccatc gggattttca ccacatcaat ggggtaacga
    58561 tttttcccgg ccacacgctg catgacatgc cagcggccat ttttcagttg ctgaataaac
    58621 gcgccgggaa tacgacggtt tcccaccaca agcacgctgc cgccaccttt cagggctgaa
    58681 cgctgcccct ttttacgacg cctgcgtcgg gacaggacaa tccgcgcgtt acccagcttt
    58741 attacgggca aatccccccg gttaaccctg attctggcct gcggattttt gaccgtggcc
    58801 cttttcagcc tggccctttc ctttaccagt ttccggcgta cctttgtctc acgggcaacc
    58861 tgtgacgccg actgcgatat cgcggatgaa gcaacgcggt taatggccat tgcggcggca
    58921 ccgggcaccg ccgttcttct gatacggctg aggttttcaa cggcctgctc aagacctttt
    58981 atggccatac atcccccttt cagcggcgac ggttaacggc aggcggtacg ccccgcccaa
    59041 gccagagatg acagcttccg ccatcatccg gcgaaatccg gtctatccag aagttttcct
    59101 caccgatggt cagcgtgtcg ccgcgccgca gctgccgcac atcatcagtc cggacaaaca
    59161 gggacgggct ggagccttca acgcgcacgc cctgtccggc atagctgata ttttcagggt
    59221 catcaaaaac accacgtatc acagcaccgg actgctcacc ggatgtcatg gtggctgacg
    59281 ttcccatgta cccgcgtatc gtttcatcgg cgcgggcaat ggcagcatcg aacaggttat
    59341 cgaaatcagc cacagcgcct cccgttattg cattctggcc aggccgcgct ctgtcatttc
    59401 agctgacaca ccggcagaga cacggaacgc cgttcccggc agcacaaatg ccacagcctc
    59461 atcccgcgtg gcgtgaagtg catcagtatg cagcgtcacc agtgccacaa ccgtgaccag
    59521 atcagccgta tcagtcacgg tatccggctg cgctgatacc acctcatttt catgtccggt
    59581 cagcgcattt tccgggctga cagatgtgtc ctgaccggca gcgtcatccg tgtcatcaat
    59641 ctcctcttcc agctctgcca cacggagcac cagttcttct ttcgtccccg tcaggctgac
    59701 atcacggttc agttgctcac ccagcacctg aagacgggca atcagttcat ctttcgtcat
    59761 ggactcctcc acagagagaa aatggccccg aagggccatg attacgccag ttgtacggac
    59821 acgaacgcat cagggtcagc cagcagcatc agcggtgctg actgaatcat ggtgaactca
    59881 cgcgccggat cgccggtggt cacccagttt ttcgggtagc gggcagaggc gttaatacct
    59941 tcgcgctgtg cgtccgcatc ctgaatgcag ccataggtgc gcagaccgcg tgcctgagtg
    60001 ttccccagca ccatcgtgtt gtccggaagg aagttctttt tgacgtcgtt ttccacgtac
    60061 tgtccggaat acacgacgat cgccgtatcg ccatacatcc ccttatagga caccgcttcg
    60121 cccaggtctt ttaccgctgt ctccagctcg gaattagagc cgcgacgggt atccagcttc
    60181 tccctgacgg ctttgaagga acggaacagc gcccagcctt tcggatcaaa cacgatgata
    60241 ttcaccactc cgctggcgtt cagcgcatag gcttcgatat cgtcggtcgg gtcatacgtg
    60301 gacttgtcac gcttgctcca ctccgtacca ccggactgtg tgatgttgtt cgccgcactg
    60361 cggcccatat ccacctcaac cggatcgaag gcttcaccgg tcatggtgta tttgccctta
    60421 agcacagcag aaactgcctg catctcttcg acctgagcaa tggccagctc ttcgtctcgc
    60481 atgttctgca tgatgatgcg acggcggcgg taagccgggt ccgccagatt ctgcggatct
    60541 tcatccggca ggcgacgcag ggtcatctgc ggattcacct catgcttcgg cttgacatat
    60601 cccggtgtaa attcagaggt ggagccgcca cgggagcgga taacctcacc ggaaacaatc
    60661 ggcgaaacgt acagcgccat gtttaccagt cccggaattt gtgagagata gactttctcc
    60721 gtggtgaagg gatagctctc acggaaaaag agacgcagaa acagcggatc aaacttaaat
    60781 ttcttctcat ttgccgccag cagctgggcg gttgtgtaca tcgacataaa aaaatcccat
    60841 aaaaaaagcc gcacaggcgg cctttagtga tgaagggtaa ggttaaacga tgctgattgc
    60901 cgttccggca aacgcggtcc gttttttcgt ctcgtcgctg gcagcctccg gccagagcac
    60961 atcctcataa cggaacgtgc cggacttgta gaacatcagt gtggtgctgg tctggtcagc
    61021 agcaaccgcc agaatgccaa cggcagcacc gtcggtggtg ccatcccacg caaccagctt
    61081 acgggtggag gtgtccagca tcagcggggt cattgcaggc gctttcgcac tcaatccgcc
    61141 gggcgcggtt gcggtatgag ccgggtcact gttgcccagc ggctggtaat gggtaaaggt
    61201 ttctttgctc gtcataaaca tcccttacac tggtgtgttc agcaaatcgt taacggcatc
    61261 agatgccggg ttacctgcag ccagcggtgc cggtgccccc tgcatcagac gatccagcgc
    61321 agtgtcactg cgcgcctgtg cactctgtgg tgctgcggcc agaatgcggc gggccgtttc
    61381 cacggtcata ccgggggttt ctgccagcac gcgggcctgt tcttcgcgtc cgtgagcctc
    61441 ctcacagttg aggatcccca taatgcggct gttttctgcc gcaaccgcag cggtgatctg
    61501 cgcgttcacg tccggctgcg ccgcgctggc gttttcgccc tccgtcgctg gcaccacgtc
    61561 agtaacgtca gcctgcgaag cagtggctga aacagttgtt gattgagtct ctttggtcat
    61621 tcgccctcct gagagacggg atttacgtgc atccagtgca tcacgcataa cggtgatcgc
    61681 atcggtgctg ttgacaagtt catcagccag tccggcatca atggcctcct gaccgctgta
    61741 cactgcagcc tcggtatcca gcacagcctg cacggacagg ccggtatatg ccgacacctt
    61801 ctgcgcaaac atccggcggg ttgcatccat ccgggactgc agtgtctccc ggacgtcatc
    61861 cggaagatgg ctgtaggggt tgccatccac cttatggctg ccgctgtaaa tcagcgtgat
    61921 ttccacgccc tgtttctcca gcgcagcgcc gtaattactg tgagccatca tgacgccgat
    61981 ggagcctgtc cgtgcggtct gcgtgaccag acgtcgggag gcggcactgg caagcagctg
    62041 acctgcactg cagttcatgt cgttggccag cgcccatacc ggctttatgt cacgcacacg
    62101 ggcgatgatg tcagcgcagt caaatgctcc cgccaccatt ccgcccggtg tgtccatatc
    62161 gagcagaatg ccgtccacca tcgggtcgct ggcagcctgt tgcagacggg cgataatgcc
    62221 gttgtaaccg gtcatccccg agtacggctg cagcgcccgc gtccggctga ccagcgtgcc
    62281 ggacaccggc agcacggcga tgccgttcat gacctgataa ctgcgggcct gtcgtggtcc
    62341 gtcatcatca ccggataatg ccagcgtcgc gggtgcctcc tgggcagtca ggctgtcgcc
    62401 ggacaccgca tccgtcaggc ggctgatccc aagctggcct gcaagcgcac aaaagaaaac
    62461 ccgcgcatag gcgggttcaa gcatcagcgg ctcattaaag gccatgctgg caatatgcgg
    62521 gagattacgc agctctgctg tcactcttct cctcctctgt tgattgtcgc agcccggatt
    62581 caaatgctgc agccgcccag gcgggcggtt taagaccagc cgcgcggcgc tccatcgttt
    62641 cacggacctg ctgggcaaaa atttcctgat agtcgtcacc gcgtttcgcg cactctttct
    62701 cgtaggtgct cagtccggct tctatcagca tcaccgcttc ctgaacttct ttcagaccat
    62761 cgatggccat acgaccggag cctatccagt cgcagttccc ccaggcactg cgggcttcct
    62821 gaaagctgaa gcgcgctttt gaaggtaacg tcaccacgcg gcgaacgatg gcctcttcca
    62881 gccagcacag aaacatctgg ctcgcctgac gggatgcgac gaattttcgc cgccccataa
    62941 agtacgccca cgactcgttc gcactggccc gtgccgtgga gtagctcatc tgggcgtaat
    63001 tccgggaaag ctgctcatac gagacaccca gcccggcagc gatataccgc agcagtgact
    63061 gctcaaacac ggagtagccg ttatccgtgt cctgagccgt ctgcaggttc agtgagtccc
    63121 ccggcatcag gtgcggcact tttgcgcctc ccagacggac cggtgctgcg gcgtaatacg
    63181 cggcaatttc accaatccag cccgtcagcc tttcccgctg ctcctgactg ttcgcgccca
    63241 gaataaaatc catcgctgac tgcgtatcca gctcactttc aatggtggcg gcatacatcg
    63301 ccttcacaat ggcgctctgc agctgcgtgt tctgcagcgt gtcgagcatc ttcatctgct
    63361 ccattacgct gtaaaacaca tttgcaccgc gggtctgccc gtcctccacg ggttcaaaaa
    63421 cgtgaatgaa cgaggcgcgc ccgccgggta actcacgggg tatccatgtc catttctgcg
    63481 gcatccagcc aggatacccg tcctcgctga cgtaatatcc cagcgccgca ccgctgtcat
    63541 taatctgcac accggcacgg cagttccggc tgtcgccggt attgttcggg ttgctgatgc
    63601 gcttcgggct gaccatccgg aactgtgtcc ggaaaagccg cgacggactg gtatcccagg
    63661 tggcctgaac gaacagttca ccgttaaagg cgtgcatggc cacaccttcc cgaatcatca
    63721 tggtaaacgt gcgttttcgc tcaacgtcaa tgcagcaaca gtcatcttcg gcaaactctt
    63781 tccatgccgc ttcaacctcg cgggaaaagg cacgggcgtc ttcctccccg atgcccagat
    63841 agcgccagct tgggcgatga ctgagccgga aaaaagaccc gacgatatga tcctgatgca
    63901 gctggatggc gttggcggca tagccgttat tgcgtaccag atcgtctgca cgggcattgc
    63961 cacgggtaaa attgggcagc agggctgcat ccacactttc acccggtggg ttccacgccc
    64021 gcaactgccc accaaatccg ctgccaccgc cgtgataacc ggcatattca cgcagcgatg
    64081 tcatgccgtc cggccccaga agggtgggaa tggtggacgt tttcatacat aaaatcctgc
    64141 aggtcccctg cgtcgctgtg tcatgccggt ctgcacttcc agctccgcaa tgtatttttt
    64201 caggtcagac acggaagtgg ccgtaaactc cactcgccgt ccgtctttct gtaccgttgc
    64261 cacccgttta cctgtcatca ggtcatgcag tgccgcacgg gcagcggcaa gttcttcctg
    64321 tcgcgtcatt catcctctcc ggataaggca cgggcgtaat ctgccagtgt tttcttgttg
    64381 gttgctgcac catcctcttc ctgcaggctc gccagcagtg cactgagatc cagctgccag
    64441 cgggaaatac tgatgcgcag cgccgccagc gcataaacga agcagtcgag cgcctcattg
    64501 cgtcgctttt tgctgtccca cagtattttt tttctgccat ccacccattt ttcgacctgc
    64561 tcttcagcag tcagctgctg cgcttcggtc agatcaaaaa tatccgggtt attcgggaag
    64621 tgaacggcac cgggaagcgg ttcatcccct tccggcgtca gtgtgaagcg gttataaatc
    64681 tgctctttcg cggtatccgt accgatttcg gtaaggtaaa ccccgttttt gtttcgctta
    64741 cgtggcatgc tggccaccgg cttaccgtag acggatgccc ctttaatggg gatcacccgg
    64801 aacagcccat gttttttcga gcgttcatac acaatggtag ggtcaatccc gccagtatcc
    64861 cagcagatac gggataccga catttctgca ccattccggc gggtataggt tttattgatg
    64921 gcctcatcca cacgctgcag cgtctgttca tcgtcgtggc ggcccataat aatctgccgg
    64981 tcaatcagcc agctttcctc acccggcccc catccccaaa cgcgcatttc gtagcgatcc
    65041 agctgggagt cgataccggc ggtcaggtaa gccacacggt caggaacggg cgctgaataa
    65101 tgctctttcc gctctgccat cacttcagca tccggacgtt caccgatttt cgcttcccac
    65161 gtctcaccga gcgtggtgtt cacgaaggtt ttacgttttc ccgtatcccc tttcgtcttc
    65221 atccagtctt tgacaatctg cacccaggtg gtgaacgggc tgtacgccgt ccagatgtga
    65281 aaggtcacgc tgtcaggcgg ttcaatctct tcaccggatg acgaaaacca gagaatgcca
    65341 tcacgggtcc agatcccggt cttttcgcag atataacggg catcagtgaa gtccagctcc
    65401 tgctgacgga tgacgcaggc gttatgctcg cagagataaa acacgctgga ggggtcatcc
    65461 ggcgtccatt tgaggccaaa cggcgtctct ttatcgccaa atttaaggta ctgctcctcc
    65521 ccgcagtgcg ggcaggcaac atgaaaacgc ataaaatgcg gggattcact ggctgcacgc
    65581 tcaatctggc aggtgcctct cactttgggc gtggagccac ggatggactt tggccagacc
    65641 gagccttcaa tacgcttatc gcccaggaac gtcggagagc cttcctgttc aatatcctca
    65701 tcaaaggcag caagttcatc ataacccgcc acatccaccg acttttcacg gtagtttttt
    65761 gccgctttac cgcccaggca ccagaagcca cgaccattgg aaaaacgctt catggtgagc
    65821 gtgttatccc ggtgcttttt gccataccac ggagccagcg ccagcagcga cggaatatcg
    65881 cggatggtcg gctcaacgtg ggttttcata aagttctcgg catcaccatc cgtcggcaac
    65941 cagataaggg tgttgcgctg cttatgctct atgaagtagg cataaacacc cagcagcatt
    66001 ttggaataac caacacgggc agacttcacc acattcacct cgcggacgta gtcactgccc
    66061 atcgcattca tgatggcccg ctgaaagggc agtgtttccc agcgcccttc ctggtatgcg
    66121 gattctttcg ggagatagta attggcatcc gcccattcaa cggcggtctg tggctccggc
    66181 cggaacagtg agcgaagccc ggcgcggaca aaatgccgca gcctgttaac ctgactgttc
    66241 gatatattca ctcagcaacc ccggtatcag ttcatccagc gcggctgctt tgttcatggc
    66301 tttgatgata tcccgtttca ggaaatcaac atgtcggttt tccagttccg gaaaacgccg
    66361 ctgcaccgac agggggatcc cgtcgagaat actggcaatt tcacctgcga tccgcgacag
    66421 cacgaaagta cagaatgcgg tttccaccac ttcagcggag tctctggcat tcttcagttc
    66481 ctgtgcgtcg gcctgcgcac gcgtaagtcg atggcgttcg tactcaatag tccctggctg
    66541 gagatctgcc tcgctggcct gccgcagttc ttcaacttcc cggcgcagct tttcgttctc
    66601 aatttcagca tccctttcgg cataccatct tataacggcg gcagagtcat aaagcacctc
    66661 attacccttg ccaccgcctc gcagaacggg cattccctgt tcctgccagt tctgaatggt
    66721 acggatactc gcaccgaaaa tgtcagccag ctgctttttg ttgacttcca ttgttcattc
    66781 cacggccaaa aacagagaaa ggaaacgaca gaggcccaaa agctcgtttt cagcacctgt
    66841 cgtttccttt cttttcaggg ggtattttaa ataaaaacat taagttacaa cgaagaagaa
    66901 cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa aacccgcgag gtcgccgccc
    66961 cgtaacctgt cggatcgccg gaaaggaccc gcaaaatgat aataattatc atctgcatgt
    67021 cacaacgtgc atctacgcca tcaaaccacg tcaaataatc aattatgacg cagatatcgt
    67081 attaattgat ctgcatcaac ttaacgtaaa aacaacttca gacaatacaa atcagcgaca
    67141 ctgaatacgg gacaacctca tgtcaacgaa gaacagaacc cgcagaacaa caacccgcaa
    67201 catccgcttt cctaaccaaa tgattgaaca aattaacatc gctcttgagc aaaaagggtc
    67261 cgggaatttc tcagcctggg tcattgaagc ctgccgccgg agactgtgct cagaaaaaag
    67321 agtttcttct gaagcaaaca aagaaaagag tgacattact gaattgctca gaaaacaggt
    67381 cagaccagat tgaagcaatt tagataatcg tgcagactac gccccctcat atcacatgga
    67441 aggtttatct atggatcagg tagtcatttt taaacaaata tttgataaag ttcgaaacga
    67501 tttaaactat caatggtttt attctgagct aaaacgtcac aatgtctcac attacattta
    67561 ctatttagcc acagagaatg ttcatattgt attaaaaaat gataatacag tgttattaaa
    67621 gggcctaaaa aacattgtgt ctgtcaaatt ttcaaaggat aggcatctta tagaaacgac
    67681 ctctaataag ctgaaatcca gagagatcac atttcaggaa tacagaagaa accttgctaa
    67741 agcaggagtt tttcggtggg ttacaaatat ccacgaacaa aaaagatatt actatacctt
    67801 tgataattca ttactattta ctgaaagcat ccagaaaact acacagatct taccacgcta
    67861 aaccataacg tccggcttct ctcactcctg agccggactg cattggttta ataaaaacca
    67921 tcaacaattg tgatttagat attcggaacc attcaaatat aacaaaaccc cgtaaaaacg
    67981 aggtttatgg ataaatttta ttattgaata catcagatta aattaatctt gacatcatag
    68041 ctttcaagac ccgtcatttt ttcccgtgcg gtaaactgaa tactggtaac ttctttcccg
    68101 gtctttttct taagttcaat aatttttttt gttatatatt cagaaatatc tgcttctgct
    68161 tttgttttta agttttcaat attcatcatt tcctctttta gtctgttatg actttccagt
    68221 tacacagtaa gtcgattata tggtgcaaac gtgtaaaaga taagatgaaa catcgcaata
    68281 atcaacatac gatagtctaa attttacaca aacagacaaa gagaattttc ctgaattatc
    68341 aatgcaatag catcaaatca actcaagagc cttattgctg cttccagaat ttcttctgaa
    68401 gtaacatgtc gatccgcggc tacataaatg actttatgat ctccggtcag agatggaaac
    68461 cctgcggcca ttacagtaag gtgtgttttt tcgccatttg gatattcacg catgatggtg
    68521 ttaactccag tcatcgctgg cactaccact gctggttcag agttaaaaaa aactatgatt
    68581 tttttcatga tgttaccgta gtatgtgagt atccatcgaa tagacaccaa gccaaaaagc
    68641 tcccgaagga gccttcattt tcactttttt aaatccaacg acagacggct ggcatttaag
    68701 tattgtgaaa tattatcaaa tgtaatcatc attgatttac aaaagataca ttttgccccg
    68761 aaaggattca tgtcagaaac atcaaaagat gatgttctat actgggaacc atgacaacac
    68821 gggcatctaa agtgaatatg gtttgtaata ttgtctacct caaagcgcca ctacatgaac
    68881 agcggcagga cctttaggtc cgttctcaat accaaattca acttcctgat tctcagttaa
    68941 tgttttgaaa tcgttgctct gaattgctga gaaatggaca aacacatctt tgctgccatc
    69001 tttcggcgtg atgaaac
//
LOCUS       DAOFQD010000009        66516 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.009, whole genome shotgun sequence.
ACCESSION   DAOFQD010000009 DAOFQD010000000
VERSION     DAOFQD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.009"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="QBF46_000606"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="QBF46_000606"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDQ6672835.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGEMLREVLHTRAFDKLNKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="QBF46_000607"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="QBF46_000607"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDQ6672836.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNISDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQS
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="QBF46_000608"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="QBF46_000608"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDQ6672837.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKSDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="QBF46_000609"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="QBF46_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDQ6672838.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="QBF46_000610"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="QBF46_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDQ6672839.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="QBF46_000611"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="QBF46_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDQ6672840.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRAKHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="QBF46_000612"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="QBF46_000612"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDQ6672841.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQILLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDNESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="QBF46_000613"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="QBF46_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDQ6672842.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSSNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="QBF46_000614"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="QBF46_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDQ6672843.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTVLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="QBF46_000615"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="QBF46_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDQ6672844.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="QBF46_000616"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="QBF46_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDQ6672845.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="QBF46_000617"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="QBF46_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDQ6672846.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16477..17757)
                     /gene="hemL"
                     /locus_tag="QBF46_000618"
     CDS             complement(16477..17757)
                     /gene="hemL"
                     /locus_tag="QBF46_000618"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDQ6672847.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17982..19403
                     /gene="clcA"
                     /locus_tag="QBF46_000619"
     CDS             17982..19403
                     /gene="clcA"
                     /locus_tag="QBF46_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDQ6672848.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19427..19492
                     /locus_tag="QBF46_000620"
     CDS             19427..19492
                     /locus_tag="QBF46_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672849.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19485..19829
                     /gene="erpA"
                     /locus_tag="QBF46_000621"
     CDS             19485..19829
                     /gene="erpA"
                     /locus_tag="QBF46_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDQ6672850.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19876..20499)
                     /locus_tag="QBF46_000622"
     CDS             complement(19876..20499)
                     /locus_tag="QBF46_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDQ6672851.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20537..21337)
                     /gene="btuF"
                     /locus_tag="QBF46_000623"
     CDS             complement(20537..21337)
                     /gene="btuF"
                     /locus_tag="QBF46_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDQ6672852.1"
                     /translation="MAKSLFRALVALSFFAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLALSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21330..22028)
                     /gene="mtnN"
                     /locus_tag="QBF46_000624"
     CDS             complement(21330..22028)
                     /gene="mtnN"
                     /locus_tag="QBF46_000624"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDQ6672853.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNIPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22112..23629
                     /gene="dgt"
                     /locus_tag="QBF46_000625"
     CDS             22112..23629
                     /gene="dgt"
                     /locus_tag="QBF46_000625"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDQ6672854.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEEQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFAELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23759..25183
                     /gene="degP"
                     /locus_tag="QBF46_000626"
     CDS             23759..25183
                     /gene="degP"
                     /locus_tag="QBF46_000626"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDQ6672855.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25338..26495
                     /gene="cdaR"
                     /locus_tag="QBF46_000627"
     CDS             25338..26495
                     /gene="cdaR"
                     /locus_tag="QBF46_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDQ6672856.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26584..26970)
                     /locus_tag="QBF46_000628"
     CDS             complement(26584..26970)
                     /locus_tag="QBF46_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDQ6672857.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27132..27944)
                     /gene="yaeI"
                     /locus_tag="QBF46_000629"
     CDS             complement(27132..27944)
                     /gene="yaeI"
                     /locus_tag="QBF46_000629"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HDQ6672858.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27999..28823)
                     /gene="dapD"
                     /locus_tag="QBF46_000630"
     CDS             complement(27999..28823)
                     /gene="dapD"
                     /locus_tag="QBF46_000630"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDQ6672859.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28854..31526)
                     /gene="glnD"
                     /locus_tag="QBF46_000631"
     CDS             complement(28854..31526)
                     /gene="glnD"
                     /locus_tag="QBF46_000631"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDQ6672860.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWHIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31588..32382)
                     /gene="map"
                     /locus_tag="QBF46_000632"
     CDS             complement(31588..32382)
                     /gene="map"
                     /locus_tag="QBF46_000632"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDQ6672861.1"
                     /translation="MAISIKTSEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32750..33475
                     /gene="rpsB"
                     /locus_tag="QBF46_000633"
     CDS             32750..33475
                     /gene="rpsB"
                     /locus_tag="QBF46_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDQ6672862.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33733..34584
                     /gene="tsf"
                     /locus_tag="QBF46_000634"
     CDS             33733..34584
                     /gene="tsf"
                     /locus_tag="QBF46_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDQ6672863.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPAKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34731..35456
                     /gene="pyrH"
                     /locus_tag="QBF46_000635"
     CDS             34731..35456
                     /gene="pyrH"
                     /locus_tag="QBF46_000635"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDQ6672864.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35748..36305
                     /gene="frr"
                     /locus_tag="QBF46_000636"
     CDS             35748..36305
                     /gene="frr"
                     /locus_tag="QBF46_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDQ6672865.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36397..37593
                     /gene="ispC"
                     /locus_tag="QBF46_000637"
     CDS             36397..37593
                     /gene="ispC"
                     /locus_tag="QBF46_000637"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDQ6672866.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37782..38540
                     /gene="ispU"
                     /locus_tag="QBF46_000638"
                     /gene_synonym="uppS"
     CDS             37782..38540
                     /gene="ispU"
                     /locus_tag="QBF46_000638"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDQ6672867.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38553..39410
                     /gene="cdsA"
                     /locus_tag="QBF46_000639"
     CDS             38553..39410
                     /gene="cdsA"
                     /locus_tag="QBF46_000639"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDQ6672868.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39422..40774
                     /gene="rseP"
                     /locus_tag="QBF46_000640"
     CDS             39422..40774
                     /gene="rseP"
                     /locus_tag="QBF46_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDQ6672869.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40804..43236
                     /gene="bamA"
                     /locus_tag="QBF46_000641"
     CDS             40804..43236
                     /gene="bamA"
                     /locus_tag="QBF46_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDQ6672870.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43358..43843
                     /gene="skp"
                     /locus_tag="QBF46_000642"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43358..43843
                     /gene="skp"
                     /locus_tag="QBF46_000642"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDQ6672871.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43847..44872
                     /gene="lpxD"
                     /locus_tag="QBF46_000643"
     CDS             43847..44872
                     /gene="lpxD"
                     /locus_tag="QBF46_000643"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDQ6672872.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44977..45432
                     /gene="fabZ"
                     /locus_tag="QBF46_000644"
     CDS             44977..45432
                     /gene="fabZ"
                     /locus_tag="QBF46_000644"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDQ6672873.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45436..46224
                     /gene="lpxA"
                     /locus_tag="QBF46_000645"
     CDS             45436..46224
                     /gene="lpxA"
                     /locus_tag="QBF46_000645"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDQ6672874.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            46224..47372
                     /gene="lpxB"
                     /locus_tag="QBF46_000646"
     CDS             46224..47372
                     /gene="lpxB"
                     /locus_tag="QBF46_000646"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDQ6672875.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47369..47965
                     /gene="rnhB"
                     /locus_tag="QBF46_000647"
     CDS             47369..47965
                     /gene="rnhB"
                     /locus_tag="QBF46_000647"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDQ6672876.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFV
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            48002..51484
                     /gene="dnaE"
                     /locus_tag="QBF46_000648"
     CDS             48002..51484
                     /gene="dnaE"
                     /locus_tag="QBF46_000648"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDQ6672877.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51497..52456
                     /gene="accA"
                     /locus_tag="QBF46_000649"
     CDS             51497..52456
                     /gene="accA"
                     /locus_tag="QBF46_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDQ6672878.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52555..54696
                     /gene="ldcC"
                     /locus_tag="QBF46_000650"
     CDS             52555..54696
                     /gene="ldcC"
                     /locus_tag="QBF46_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDQ6672879.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVVQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54753..55142
                     /locus_tag="QBF46_000651"
     CDS             54753..55142
                     /locus_tag="QBF46_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDQ6672880.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            55207..56505
                     /gene="tilS"
                     /locus_tag="QBF46_000652"
     CDS             55207..56505
                     /gene="tilS"
                     /locus_tag="QBF46_000652"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDQ6672881.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVAL
                     RAIHVHHGLSANADTWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLVDDLAHCQTPQGTLQIAPMMTMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQAWLQPLELPA
                     GLGSVQLTAGVDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(56554..56814)
                     /gene="rof"
                     /locus_tag="QBF46_000653"
     CDS             complement(56554..56814)
                     /gene="rof"
                     /locus_tag="QBF46_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDQ6672882.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56801..57001)
                     /locus_tag="QBF46_000654"
     CDS             complement(56801..57001)
                     /locus_tag="QBF46_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDQ6672883.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            57167..57712
                     /locus_tag="QBF46_000655"
     CDS             57167..57712
                     /locus_tag="QBF46_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDQ6672884.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKFVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57709..58131
                     /gene="arfB"
                     /locus_tag="QBF46_000656"
     CDS             57709..58131
                     /gene="arfB"
                     /locus_tag="QBF46_000656"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDQ6672885.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            58145..58855
                     /gene="nlpE"
                     /locus_tag="QBF46_000657"
                     /gene_synonym="cutF"
     CDS             58145..58855
                     /gene="nlpE"
                     /locus_tag="QBF46_000657"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDQ6672886.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(59010..59834)
                     /locus_tag="QBF46_000658"
     CDS             complement(59010..59834)
                     /locus_tag="QBF46_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDQ6672887.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSADFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59887..61605)
                     /gene="proS"
                     /locus_tag="QBF46_000659"
     CDS             complement(59887..61605)
                     /gene="proS"
                     /locus_tag="QBF46_000659"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDQ6672888.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTITELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61716..62423)
                     /gene="tsaA"
                     /locus_tag="QBF46_000660"
     CDS             complement(61716..62423)
                     /gene="tsaA"
                     /locus_tag="QBF46_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDQ6672889.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLMAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62420..62824)
                     /gene="rcsF"
                     /locus_tag="QBF46_000661"
     CDS             complement(62420..62824)
                     /gene="rcsF"
                     /locus_tag="QBF46_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDQ6672890.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62942..63757)
                     /gene="metQ"
                     /locus_tag="QBF46_000662"
     CDS             complement(62942..63757)
                     /gene="metQ"
                     /locus_tag="QBF46_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDQ6672891.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63797..64450)
                     /gene="metI"
                     /locus_tag="QBF46_000663"
     CDS             complement(63797..64450)
                     /gene="metI"
                     /locus_tag="QBF46_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDQ6672892.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64443..65474)
                     /gene="metN"
                     /locus_tag="QBF46_000664"
     CDS             complement(64443..65474)
                     /gene="metN"
                     /locus_tag="QBF46_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDQ6672893.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65662..66234
                     /gene="gmhB"
                     /locus_tag="QBF46_000665"
     CDS             65662..66234
                     /gene="gmhB"
                     /locus_tag="QBF46_000665"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDQ6672894.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15696 a        16565 c        18318 g        15937 t
ORIGIN      
        1 tggaattgat atttgtttat aggaagttat attatttccg caaagagatg tttaaactaa
       61 aatcgtaatt aaggaaagag atgagatatg attttcagaa aatatttata ttcgcaatat
      121 aaataaatta ccatttgttt aatttgtcaa atgctcttgt atgtaagact tcacgcaaca
      181 tctccccatc aggaaacacc aactccggcg cgatttcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc agtatttatc acttcattac
      301 caaacagcat gatgtcgaga tccagcgtgc gtggtcccca gcgttcagct ttgcggacgc
      361 gaccttgctg caattcaata cgctgtgtgt gattgagtag ctcttcaggt gcaagagagg
      421 tttccagcgc cacggctgcg tttaagtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gtaagaatgt ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgcg gtaccctcac gacgtggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttgag catccctttt tggtctggtg gcgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcgttacgct caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttactc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaatgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caggaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc atgccgccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga tgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatca catgagccag
     1681 acggaaacgg cgacccacaa ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa agtctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttattgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgct
     2101 gactcaggca cggcggtaag ccgccagttt ttgacggctg actggaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgacccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgctttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg agatttgccg cactgtcggt tcgatcaaat ctgccccgcg gacaatttcg
     2401 ctcacgccct ggaagtgatc atccaccacc accgccaggt tataggcaaa caaaccatca
     2461 cggcgatgaa taataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgccgcgc
     2521 agctggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtgc aggtgcagta ataacttagc ccttgctcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatac gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccaagc
     2881 gcagcaatca gagaaccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tagtgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaagataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttctgcg
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtgt tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggattgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctcggcgct cacttcgcct aaaaatgcca gagtcagatg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtttgg cgcgccagtg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtgccg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcagatatt attaagtgcg ccgaccggag ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctcct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggccaaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcgcatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga atttcatgag cgcagcttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 acgaccgctt gcagcaaatg ctgccagaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccattat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg ctgcgtcagg aaagcggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgt gtgcaggaac aactggcttc gcgcatcggc agtgatgtgt
     5701 tgctctgccc gctgtatggc gcgttgtcgc tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgt tttgagccgc
     5881 gcacggggct tacgcgactg attactcaac gcgttagcca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tctgcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc tgccgcgcaa agtgaaccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccagtagt gaatctaatg gccgcgaaac gtctgttaca aatgctgggg gcgctggacg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttagcgcg aagaacgacg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cggcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctgcaaatcg gtcagttgac ggtgaaagtg cagccgttag
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggcctg cggttgataa tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtgcattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccaccaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctggagtt gctttcccct gcccaaaggc
     7261 cattacaaat cacacgagat ttgagcgcct tctggaaagg agcgtaccgt gaggtgcaaa
     7321 aagagatgaa agggcgttat cccaaacatg tctggccgga cgacccggca aacactgcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagat
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcac aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cctgcggcag
     7921 tttatggccg aatggtcaat cttgagccag acatgaccat cagcaagaac gagatggtga
     7981 agctgctgga ggcgacccag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc taacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagcaaag
     8101 aagggcaggt gcgtgcgcgt ttgacctttg atggcgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggcttcttcc gtcttgatcc gcgcctgatc accatgatct
     8221 cctcgccaaa cggtgaacaa cgtctgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgacggc atcagcctct
     8341 actccatcgg acgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgc tcttactggc
     8461 gtaaagcgaa cgaagcctat atggcgttga tcatggatgc gcgttacagc aaagatcgca
     8521 ttcttgagct gtatatgaat gaggtgtatc tcggtcagag cggcgacaac gaaattcgtg
     8581 gcttcccgct ggcgagcctg tactactttg gtcgcccggt agaagagttg agccttgacc
     8641 agcaggcgct gttagttggc atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgttt gctacaacaa cagcagatta
     8761 tcgatggtga actgtatgaa atgctcagcg cgcgtcctct gggcgtacag ccgcgcggtg
     8821 gggtaatctc tccacagcca gcctttatgc aactggtgcg tcaggagttg caggcgaaat
     8881 taggcgataa ggtaaaagat ctgtccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agctgagcga tctggaaacc gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac tgccttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgcggatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgatcgtc gttatagcga aagtggcaga gtgatgctgg
     9301 tggatgcgtt gacccgttcg atgaacgtgc cgacggtaaa tctggggatg gcactggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtgcc gaaagatcag ctgcatccgg
     9421 ttccggcgat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggcggt aaccgtgcac cgctttctgc gctgcgttcg gtaatcgcgg
     9541 aagatggcaa agtgctgtat cagagcttcc cgcaggcgga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgcggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactact aacaataacg
     9721 tagatacctg gtttgcgggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acaccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagtgagat gcagcagcag ccgtcaagca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggattaaaga tatgtttggt agtaattaac
    10141 gtctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccacccaaa
    10201 gtattaaccc ttttttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta tcattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcga cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc cagaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gatccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagtagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctc aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgctgg atgatgacgg cgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccgtc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgctcaat ctgtacaatc cggtgaatac cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa gcagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctgggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ccgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaacaaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgctcg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgttgtgggc tgactacacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agttatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtacgttatg atctggcgcg agtcggcatg gctggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc ttccgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggccgtc atcagccgcc
    12841 gtcggaaggg gagattcttc ttgatgccca accgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggggcggc
    13021 agatcgcgaa aaagtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacctcg gcgctggata tcgcccacca
    13201 ggttgatgtg ctgtcgctgg tgcaccgttt aagtcaggag cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcgg
    13321 cggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttccccgt tgttatggca gatgaatacc gcccacgcgg cggctattga tcccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgatcgacg tcggtttgcg cacagaacct aaccttgaac tgctgaccga aatgaaacca
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcaccag aaatgctggc tcgtattgcg
    13801 ccggggcgtg gatttaactt cagcgatggc aaacagccgc tggcgatggc gcgtaagtca
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cagaaacgca tttagcgcac
    13921 tatgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtga acgcccgtta
    13981 ttgctgacga cgcttatcga tccgcgccat atgctggttt tcggtccaaa cagcttgttt
    14041 caggaaattc ttgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagcat cgatcgtctg gcggcgtata aagatgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggac gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgcgccgg acgctttcag cgcgtacccg cagtctggtt ttatggtgcg
    14281 acgctctcgg caatgcactt tgtgcgcgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttt ccggcgttat tgctggcgct gctggtgatt gtcgctacgg
    14401 cgctgacctg gatgaacttt tcgcaggcgc tgccgcgtag ccagtgggcg caggccgcct
    14461 ggtcgccgga tattaacgtc atcgagcaga tgatttttca ctacagcttg ttaccacgtc
    14521 tggcgatttc tctactggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tgctgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgctacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ccggtgctat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggcttt
    14761 cgccggttac gctgattctc gcggggctgg tagtgagcct ttattgtggg gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgttgccgcg tttacgcagc attagcgcgc cggatatgaa ggtcaacgat cgtgtcgcgg
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgctg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtggcgggc tgtattattc agcgactgac cggaaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggcgt ggtgttgatg ctgtttctgg
    15661 tgccgggtaa tgcctttggc tggctgttac ctgcaggcag tctcggcgcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggccgcg gtggattttc cccacaccgt atgttactgg
    15781 cggggatggc gttaagcacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 cgcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattacc ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc ccgagccgta ggaatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gttttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccgtt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggcgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 ttttccatat aacggccctg tacgcctgga cggataaagc gtttacgcga caacggatgc
    16381 ccgatgcgac gctggcgcgt cttatcgggc ctacaaacgg ccccgaatcg taggtcggat
    16441 aaggcgttca cgccgcatcc gacaaaccat gctggatcac aacttcgcaa acacccgacg
    16501 tgcagcatcg atggtgttat tgatatcttc catgctgtgc gccacggaca taaagcccgc
    16561 ttcaaacgct gacggtgcca ggtaaacacc ttcgtccagc atcatatgga agaaacgctt
    16621 aaagcgttcc acgtcacagg ccatcacatc ctgatagcac gtcacggact cggcgtcggt
    16681 aaagaaaata ccgaacatgc cgccaacgtg gttaacgacc agcggaattc cggcttcttc
    16741 tgccgcttcc agcagacctt ctgccagacg tgttgtcagc tcatccagcg tttcgtgaac
    16801 gcccggctgc gcgacttcat tcagacaggc gaaacccgct gccatcgcaa tcgggttacc
    16861 ggaaagcgta cccgcctgat agaccggacc cgtcggggcc agcgcatcca ttacatcacg
    16921 acgaccaccg aatgcgccta ccggcattcc accgccgatg attttgccga ggcaggttaa
    16981 atctggcact acgccgtaat aatcctgtgc gccagctagc gctacgcgga aaccggtcat
    17041 cacttcatcg atgatcagca acgcgccaaa ttcgtcgcac agcgcgcgca gacctggcag
    17101 gaactctggc agcggcggaa cacagttcat attgcctgcc accggctcga cgataataca
    17161 ggcaatctct tgcgggtatt gctcaaatgc ggcgcgtaca gaagccagat cattataagt
    17221 acaggttaag gtatgtttgg cgaaatctgc cggaacgccc ggcgagtttg gctggcctaa
    17281 cgtgagtgcg ccagaaccgg ctttcaccag caggcagtca gcgtgaccat ggtaacaccc
    17341 ttcaaattta ataattttgt cgcgaccggt aaaaccacgg gccaggcgga tggcgctcat
    17401 ggtcgcttca gtgccggagt tcaccatgcg caccatatcc atggtcggga ccagttcggt
    17461 caccagttgc gccattttca cttccatttc ggttggtgca ccaaagctta aaccacgctc
    17521 ggcggcttca atcacggcat tgcggattgc cggatggtta tggcccagca ccatcggccc
    17581 ccaggaaccg acataatcga tataggcttt gccatcaaca tcgtacagat aagcgccgtc
    17641 cgctttttcg ataaacagtg gagtgccgcc cacgccagta aaggcgcgaa caggggagtt
    17701 cacaccgcca gggatcagct cgcgcgctgc gctgtaaaga ttttcagact tactcatgga
    17761 gggttcctga ttcgtagaaa aagtgaatgg ctgctattct atgttattca taacaagtta
    17821 aatactcgtc aaacatcagg ctgcttgtac tggtgcaatc ctgatttctt agagtataaa
    17881 agttttgtgc atttgaaaca ttacgctttg caaaggattt tcatggaacg tgcgagtaaa
    17941 atgccgtcat cttatttgta tgaccaataa gtgatcattg gatgaaaact gatactccct
    18001 ctttagaaac accgcaggcc gcgcgcctgc gacgcagaca actgattcgc caacttcttg
    18061 agcgcgataa aaccccgtta gccattttgt ttatggcggc agtcgtcggc acgcttgttg
    18121 ggctggcagc ggttgctttt gacaaaggtg tcgcctggtt gcagaaccaa cgtatggggg
    18181 cgctggtaca tactgctgat aattatccgc ttctgttaac cgtcgctttt ctctgttcgg
    18241 cggtgctggc gatgtttggc tactttttgg tgcgcaaata cgcgccggaa gcaggtggtt
    18301 cggggatccc ggaaattgaa ggggcgctgg aagatcaacg tcccgttcgc tggtggcgtg
    18361 tattgccggt gaagttcttt ggcgggctgg ggacactcgg cggaggcatg gtgttggggc
    18421 gcgaagggcc aaccgtgcag atcggcggta acattggccg tatggtgctt gatattttcc
    18481 gcctgaaagg tgacgaagct cgccatacgc tgctggcaac cggtgctgct gcggggctgg
    18541 ctgcggcctt taacgcgccg ctggcgggta ttttgtttat tatcgaagag atgcgtccgc
    18601 agtttcgcta tacgttaatt tcgattaaag cggtatttat tggtgtcatt atgtcgacca
    18661 ttatgtaccg gatttttaat catgaagttg cgttgattga cgtcggtaaa ctttctgatg
    18721 cgccgcttaa tacgctgtgg ctttatctga tcctcggtat tatttttggc attttcggcc
    18781 ctatttttaa taaatgggtg ctggggatgc aggatttgct gcaccgtgtg cacggcggca
    18841 atattaccaa atgggtgcta atgggcggtg cgattggcgg tctgtgtgga ttgctggggt
    18901 ttgtggcacc agcaacgtcg ggcggcggtt ttaacctgat tcctatcgct accgcgggga
    18961 atttcagcat gggaatgctg gtgtttatct tcgtcgcgcg ggtcattacc accttactct
    19021 gcttctcttc cggcgcgccg ggcggtattt ttgccccgat gctggcgctg ggtactgtgc
    19081 tgggaaccgc tttcggaatg gttgccgttg agctgtttcc gcaatatcac cttgaggcgg
    19141 ggacgtttgc tattgccgga atgggggcat tactggcggc atctattcgc gcgccgttaa
    19201 cggggatcat tctggttctg gagatgaccg ataactacca gctcattttg ccaatgatta
    19261 ttaccggtct tggcgcaaca ctattagcgc aatttaccgg cgggaaaccg ctatactcgg
    19321 cgattcttgc gcgcacgctg gcaaaacagg aagctgagca actggcgcga agcaaggccg
    19381 catcagccag cgagaatact tgaacgaaat accagggtat tagataatgg cgattattat
    19441 tgggttagaa tttgcccaat tgccgatgtc gtttggagca aaatatgagt gatgacgtag
    19501 cactgccgct ggagtttacc gacgcagcag ccaacaaagt taaaagcctg atcgctgacg
    19561 aagataaccc gaatctgaaa ttacgcgtgt atatcaccgg tggcggttgc agcggcttcc
    19621 agtatggttt cacctttgat gatcaggtga acgaaggcga tatgaccatc gaaaaacagg
    19681 gcgttggcct ggtggttgat ccgatgagcc tgcaatatct ggtcggcggt tccgttgatt
    19741 ataccgaagg tctggaaggt tctcgtttca tcgtgaccaa cccgaacgcg aaaagcacct
    19801 gcggttgcgg ttcttccttt agtatctaat ctgttgttca tgtgccggat aaactatccg
    19861 gcacatcttc tctcttcaac gcccattctc atccagcgca aacgtcggta gcttaagatg
    19921 ccaacgaata gccgccagcc gaatcaatag cgtcacgacc atgcccatca tactggctgt
    19981 ttccagtggt acggaaaatg tgtaataagc cgtagcgtgg acaataccgc cgataataca
    20041 ggcagttgcg tagatttctg tacgtaaaat catggggatt tcgcgggcca gaacatcacg
    20101 aatgatcccg ccgccaacgc cagtaatgac gcccatacaa accgcgatta acggaccggc
    20161 ttccgcatta aaggctttat tcacgccaat gccgacaaac accgccagac caacggcgtc
    20221 cagcaccggc aacatccatt ttggtaagcg tcttggctgg cgcaccagca cgatggtcag
    20281 catgctggtg accattgcaa cgaccagatc ggtgggatct ttcacccaaa ataccgggcc
    20341 gtgatccagc gccatgtcgc gaattgtccc gccgcctact gcggttacca cgcccagtac
    20401 cagaacacca aaagggtcca tacgcaattt tccggctaac aaaacgccgg agatggcaaa
    20461 taccgctgtg ccgactatat ccagccaata gacgagcatt gttcaatccc cactgagcac
    20521 ctgaaaaggt caggcgctaa tctacctgtg aaagcgcatt acagagctgt tgtgcagcga
    20581 ggataatacg tgggcttgca cgttcaaacc agtcactcgt gagaggaata acgggaattt
    20641 tgagctgttc accccagtat tgtttgattt taggaatttg gtccggtccg cctgtaatga
    20701 caatcgcctg tggcgagagt gctaacacct gttcgcggct aacttgcggc cagggaaccc
    20761 ggctgtcttt aaagatgttt tctccgccac aaacttcgag tacctggttc tgaatcgact
    20821 cttttccact ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt tttttaggtt
    20881 tatccgcata ttgcgctttc aattgcgcgt actgatccag cagggattgc gcggcttgtt
    20941 cggccttgtc tggttgcgga ctccaggggg ccagttgacg taacgcattg gcaatttgtt
    21001 caatgcttgt cgcatcgacc cacatcactt ttattcccag cgaagccagc tggtcaacct
    21061 gccgctcggc attacctcca cgccaggcaa tcaccagatc gggtttcagc gcgacaatgc
    21121 gttccagatt catcccctgc caggtggaaa cctgctcaat cttttgcgct tgtggaggat
    21181 agtcggaata gctgctgacc ccaaccggcg tgatcccggc ggcaaaggca agttcagtgt
    21241 tggcgggaga aagcgtgatg acgcgcggcg cggcgttgag ccacagcggc gcgaaaaaag
    21301 acagggcgac cagcgccctg aacagtgact tagccatgtg ccagtttctg caccagtgac
    21361 tcaaccatca ggctggactg tttagcggca acagccagga actcatcgaa gctaagatga
    21421 gactgttgat cggccacgtc ggagatggcg cgtacgacaa caaacgggat gttgaaattg
    21481 tggcagacat gggcgattgc cgtcgcttcc atctctacag caatggcctg tgggaagttg
    21541 tggcggattt tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct aacaatcagg
    21601 ccacgtacag cgttaagatt cagttcggca atgcaggcct cagcggcagc gatcagttta
    21661 tcgtcagctt taaagcctgc cggacagcct ggtaactgac cgtattcata accaaatgcc
    21721 gtgacatccg cgtcgtgata acgcgcttcg tccgagacaa cgatatcgcc cactttcaac
    21781 gttggtgcca ggccaccggc agaaccggtg ttaataatca catctggctt gcagtgttcc
    21841 aacagcaaag tggcacccag cgccgcagcg actttaccga tgcccgattt cagaagcgca
    21901 acctcggttc cattcagttg gccggtatag atttcgcaac cgccgagact gatagtttga
    21961 cggttttcga ttttgtcacg cagcagcgta acttcttctt ccattgcacc aatgatgccg
    22021 attttcatag atttactcgc gataagcccg atttgaaggc atagtttacc atgcgcttac
    22081 ggggaagcgt atttctcacg cgggagagga catggcacag attgatttcc gaaaaaaaat
    22141 aaactggcat cgtcgttacc gttcaccgca gggcgttaaa accgaacatg agatcctgcg
    22201 gatcttcgag agcgatcgcg ggcgtatcat caactctccg gcaattcgtc gtctgcaaca
    22261 aaagacccag gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc ttacccactc
    22321 gatggaagtc cagcaggtgg ggcgctacat cgccaaagaa attttaagcc gtctgaaaga
    22381 gcttaaattg ctggaagcat acggcctgga tgaactgacc ggaccttttg aaagcattgt
    22441 tgagatgtca tgcctgatgc acgatattgg caatccgccg tttggtcatt ttggcgaagc
    22501 ggcgataaat gactggtttc gccagcgttt atacccggaa gatgccgaaa gccagcctct
    22561 gactgacgat cgctgcagcg tggcagcact acgtttacgg gacggggaag aaccgcttaa
    22621 cgagctgcgg cgcaagattc gtcaggactt atgtcatttt gaggggaatg cacaaggcat
    22681 tcgtctggtg catacattga tgaggatgaa tctcacttgg gcacaggttg gcggtatttt
    22741 aaaatatacc cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc actatttaat
    22801 gaaaaagccg ggttattatc tttctgaaga agcctatatt gcccggttgc gtaaagaact
    22861 taatttggcg ctttacagtc gttttccatt aacgtggatt atggaagctg ccgacgacat
    22921 ctcctattgt gtggcagacc ttgaagatgc ggtagagaaa agaatattta ccgttgagca
    22981 gctttatcat catttgcatg aggcgtgggg ccagcatgag aaaggttcgc tcttttcgct
    23041 ggtggttgaa aatgcctggg aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga
    23101 acagtttttt atgtatttac gggtaaacac cctaaataaa ctggtaccct acgcggcaca
    23161 acgatttatt gataatctgc ctgcgatttt cgccggaacg tttaatcatg cattattgga
    23221 agatgccagc gaatgcagcg atcttcttaa gctatataaa aatgtcgctg taaaacatgt
    23281 gtttagccat ccagatgtcg agcagcttga attgcagggc tatcgggtca ttagcggatt
    23341 attagagatt tatcgtcctt tattaagcct gtcgttatcc gactttgctg aactggtaga
    23401 aaaagaacgg gtgaaacgtt tccctattga aacgcgctta ttccacaaac tctcgacgcg
    23461 ccatcggctg gcttatgtcg aggctgtcag taaattacca tcagattctc ctgagtttcc
    23521 gctatgggaa tattattacc gttgccgcct gctgcaggat tatatcagcg gtatgaccga
    23581 cctctatgcg tgggatgaat accgacgtct gatggccgta gaacaataac caggcttttg
    23641 taaagacgaa caataaattt ttaccttttg cagaaacttt agttcggaac ttcgcgctat
    23701 aaaacgaatc tgaagaacac agcaattttg cgttatctgt taatcgagac tgaaatacat
    23761 gaaaaaaacc acattagcac tgagtgcact ggctctgagt ttaggtttgg cgttatctcc
    23821 gctctctgca acggcggctg agacttcttc agcaacgaca gcccagcaga tgccaagcct
    23881 tgcaccgatg ctcgaaaagg tgatgccttc agtggtcagc attaacgtag aaggtagcac
    23941 aaccgttaat acgccgcgta tgccgcgtaa tttccagcag ttcttcggtg atgattctcc
    24001 gttctgccag gaaggttctc cgttccagag ctctccgttc tgccagggtg gccagggcgg
    24061 taatggtggc ggccagcaac agaaattcat ggcgctgggt tccggcgtca tcattgatgc
    24121 cgataaaggc tatgtcgtca ccaacaacca cgttgttgat aacgcgacgg tgattaaagt
    24181 ccagctgagc gatggtcgta agttcgacgc gaagatggtt ggcaaagatc cgcgctctga
    24241 tatcgcgctg atccaaatcc agaacccgaa aaacctgacc gcaattaaga tggcggattc
    24301 tgatgcactg cgcgtgggtg attacaccgt agcgattggt aacccgtttg gtctgggcga
    24361 gacggtaact tccgggattg tctctgcgct ggggcgtagc ggcctgaatg ccgaaaacta
    24421 cgaaaacttc atccagaccg atgcagcgat caaccgtggt aactccggtg gtgcgctggt
    24481 taacctgaac ggcgaactga tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa
    24541 catcggtatc ggttttgcta tcccgagtaa catggtgaaa aacctgacct cgcagatggt
    24601 ggaatacggc caggtgaaac gcggtgagct gggtattatg gggactgagc tgaattccga
    24661 actggcgaaa gcgatgaaag ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc
    24721 taattcctcc gctgcaaaag cgggcattaa agcgggtgat gtgatcacct cactgaacgg
    24781 taagccaatc agcagctttg ccgcactgcg tgctcaggtg gggactatgc cggtgggcag
    24841 caaactgacc ctgggcttgc tgcgcgacgg taagcaggtc aacgtgaatc tggaactgca
    24901 gcagagcagc cagaatcagg ttgattccag ctccatcttc aacggcattg aaggtgctga
    24961 gatgagcaac aaaggcaaag atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc
    25021 ggctgcgcag atcggcctga agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt
    25081 gaaaaacatc gctgaactgc gtaaagttct cgacagcaaa ccgtctgtgc tggcactgaa
    25141 cattcagcgc ggcgacagca ccatctacct gttaatgcag taatctccct caaccccttc
    25201 ctgaaaacgg gaaggggttc tccttacaat ctgtgaactt caccacaact ccatacatct
    25261 tcatcatcct ttaggcattt gcacaatgcc gtacgttacg tacttcctta tgctaagccg
    25321 tgcataacgg aggacttatg gctggctggc atcttgatac caaaatggcg caggatatcg
    25381 tggcacgtac catgcgcatc atcgatacca atatcaacgt aatggatgcc cgtgggcgaa
    25441 ttatcggcag cggcgatcgt gagcgtattg gtgaattgca cgaaggtgca ttgctggtac
    25501 tttcacaggg acgagtcgtc gatatcgatg acgcggtagc acgtcatctg cacggtgtgc
    25561 ggcaggggat taatctaccg ttacggctgg aaggtgaaat tgtcggcgta attggcctga
    25621 caggtgaacc agagaatctg cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga
    25681 tgctggaaca gtcgcggttg atgcacttgt tggcgcagga tagccgtttg cgggaagaac
    25741 tggtgatgaa cctgattcag gcagaggaga atactcccgc acttactgaa tgggcgcaac
    25801 ggctggggat cgatctcaat caaccgcgag tagtggctat tgttgaggtc gacagcggtc
    25861 agcttggtgt ggacagcgca atggcggagt tacaacaact gcaaaacgcg ctgactacgc
    25921 ccgagcgtaa taatctggtg gcaattgtct cgctgaccga aatggtggtg ttgaaaccgg
    25981 cgttgaactc ttttgggcgc tgggatgcag aagatcatcg taagcgagtt gaacaactga
    26041 ttacccgcat gaaagagtac ggccagctgc gttttcgcgt ttcactgggc aactatttta
    26101 ccggtcctgg cagtattgcc cgatcctatc gtacggcgaa aacgacgatg gtggtgggta
    26161 aacagcggat gccagaaagt cgctgctatt tttatcagga tctgatgtta cctgtgttac
    26221 tcgacagttt gcgtggcgac tggcaggcca acgaactggc gcgaccgctg gcgcggctga
    26281 aagcgatgga caataacggc ttgctgcgac gaacgctggc ggcgtggttt cgtcacaatg
    26341 tgcaaccgct ggcaacgtca aaggcgttgt ttattcatcg taataccctg gagtatcggc
    26401 ttaatcgtat atcggaactg accgggcttg atttgggcaa ttttgatgac aggttgctgc
    26461 tgtatgtggc gttacaactg gatgaagagc ggtaggttat gcgttaaggt tgaggcggcg
    26521 ctggctcatt cccctcagcc cgaaggggag aggggaatgc ccgagttaac cgtcaattcg
    26581 ggattattta cgcgttagtt tttccagatc ggcttcaatc tcgctgatct tattggttac
    26641 gactgactcc aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg
    26701 gtcacgctgg cagatttgat caagctcatc aatgatatac cgtagattcg ggctgatttc
    26761 ctggacttct ttataaccct gacccacacc atcagcgacg accgttttac gctgacgcgg
    26821 atatttaaac ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga aatagatttt
    26881 cagaatatcg ttgttggctt cctgccggag gctgtaacga tcaatttctt caggattggt
    26941 aatacccaga cttttcagat tgtcgtacat agcgttacct caaaatgagt cagtaaattg
    27001 tgcttatttt agcatttggc ctggcccgcc cggctcgact gtgatcgcag aaagcctttt
    27061 tcttccagac ttctatcatg gcgtaaaaat caaaaaatta cctgctttat tgtggtgata
    27121 aaattcacgt tctacaccag ttccagcatc gttacttccg ggcggcagtt cagacgcaaa
    27181 ccatacaaac tgcccacgcc acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat
    27241 ccggcgacgt aacgtttatc ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc
    27301 agttgcccgc cgtgagtatg gccgcacagc atcagatccc agggttcatc acgcatgact
    27361 tctttgctgt cgggattatg tgccagcatc agtcttggca gatttgcttc gctggcggga
    27421 ggtggtttgc attgtccggc ccataagtca ccggtgccta ccagttcgaa ttgcctgttc
    27481 ggcgtggcga tcaccgtagc ctggttaaac aacaccgtga tgcccactga tttcaacgtc
    27541 tcgccaatta agtgattttt ttctgtacca acagggcgat cgtggttgcc gaagcaggca
    27601 aacgtcggcg cacattcggc aaggggggag aggacatcac taaacgccga aaaattcagc
    27661 ggcatatcaa ataatacgta atcgccgccc agtaatatca aatcgggctt ttgttctatg
    27721 ccaagagcaa tcgcgtcaga aatcaggctt aaaggaacaa aacgagagta atggagatcg
    27781 gccagaaaaa gaattttgaa tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg
    27841 attaattcaa accagcctgg ctcccagtaa tgcatataac cgaaacctga gctcgtggct
    27901 atcgtcgcgg cagtagcctg caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa
    27961 aaaaatcggg cagcgtcgtg ctgcccgtgt gcatactttt agtcgatggt gcgcagcagt
    28021 tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt tcttaacgat aaccgcacag
    28081 tagaggctgt atttgccatc ttttgacggc agattacctg aaacaaccac agaccccgcc
    28141 ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt gctctgacca
    28201 atgtatacgc ccatggaaat gacggaacct tcttcgacaa tcaccccttc aaccacttca
    28261 gagcgcgcgc cgatgaagca attatcttca atgatggttg ggttagcctg cagcggttcc
    28321 agcacgccgc cgatgcccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa
    28381 gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgttg
    28441 acgtaagacg gcatcagcac ggtgttacgg gcaataaacg caccctgacg taccgccgct
    28501 ggtggcacaa cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc
    28561 atcggcactt tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata
    28621 cggaaagaga gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt
    28681 ttttccgcta cacgcagtgc gccggaatcc agcagggcga tcacctgatt taccgcttcg
    28741 cgggtaacgg tgtctgcatt ggctggcgtg atctcggcac ggcgttcaaa agcggtttca
    28801 ataatgttct gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct
    28861 ttatcgtttg gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta
    28921 ttaagtgcac ggcggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg
    28981 gttgtaattc gggcaccatg aagcgaaatt cccagatcgg caaaaatttt cccgactctc
    29041 gccagcagtc ctggctggtc gagcgcgata agctcaagga acgatttgcg gtcggtatgg
    29101 gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc gtaatttggc gggttggcga
    29161 cggggctgcg gtggctgcca gctactttgc gtcagtactt gctccagacc aaaccgaata
    29221 acctcatgac gatctgcgga cagcgggctg ccatcgggtt ccagcacgat aaaggtatcc
    29281 atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg
    29341 tctaattcgg cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc
    29401 tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca gcggtttgct taaatcatgc
    29461 tgtaataaat ggcgggcatg ccaggccagt tgatttgggc tatggcggac aaaatagtta
    29521 gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt
    29581 agtgccagtg cctggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc
    29641 attccgcgtc gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc
    29701 cagctattcc acagcgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga
    29761 tagcgcagac gattttccgt ttgaacttct tcggcaaact gcttgatgac ttcagggtcc
    29821 tgaatatcgc ggcgttgtgc tgtcaccgac atcagcaagt gctggcgaac cagccaggca
    29881 accagctgtg tttcgcgtga gttcagcccg tggagttctg caaaatgcac tacatcctga
    29941 gcaccgagaa tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg
    30001 atgaaaatca gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc
    30061 tggcgcgttt cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca
    30121 tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc
    30181 ggcatatacg cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct
    30241 ccggggtgac gcagaatgct caaaaacagt tttcgtgctt ccggaatatt acacagcggt
    30301 tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg tggtggagta aatgccggtg
    30361 atcgcactgt tgcgcaccat ggtgtagaac atacgtaaga tagcttccgg ctggcgcata
    30421 aatagtgttt catcacgcag gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt
    30481 ggacgtggtt tttcgtcggc gggaagggcg aggatggctt catcgaacag ttgcagcagc
    30541 atctggttga gttcactgac gcggcgtgtc acgcggaagt aatccttcat catccgctcg
    30601 accggttcgt taccttcgcc actgtaattt agacgctggg cgacgctaag ctggcgatcg
    30661 aataacaggc gattatcgta acggctgacg accagatgca gggcaaagcg aatatgccac
    30721 aatatatgca gacattcgtt taattccgcc cgctccgctg aggttaagaa gccaaaccca
    30781 accatttcat ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg
    30841 atatcgcgca agccgccagg gctacttttg atgtctggtt caaggttgta gctggtgcca
    30901 tggtaacgct gatggcgctg gttctgttct tcaactttcg ctgcgtagaa cttgtcggaa
    30961 ggccagaatc cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc aacatcgcca
    31021 attaataagc gggattcgat taaattggtg gcgacggtta aatccgataa cccttccagc
    31081 atgcactctt caagcgtgcg cacactatga ccgacttcca gctttacatc ccagagcagc
    31141 gttaacagct cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc
    31201 agtaaatcga tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcaacc
    31261 aatgccaggt cggcaatctg gctgaatccc gcttcaatcc ataatcgttg caggagctgg
    31321 tctatgaact cggtacgtgc ctcaatcaac tgttctgcag agatcccatt gtcaaaggca
    31381 tcacccagcc aacgctggaa agtatcgata tgggctttta tcccacagac ggttaattca
    31441 tcacggggcc aggcgcatgg attttgcggt tgaccgggca gggtggggag agcggtgttt
    31501 gcgtactgtt ctggaagggt attcattgtg cgccacccat aagattaaat tatcgcatta
    31561 aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc gagattatcg ccgggatggt
    31621 gtcatccttg cgtagcgtca aaatttcgca gccgttatca gtcaccacaa tagtatgctc
    31681 atattgtgca gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg
    31741 gatctctttt ttacccgcgt tgaccattgg ctcaatggtg aacgtcatcc ccggtttcag
    31801 cacgacgttg gtttcacggg agtcatagtg cagcacctgc ggttcttcat ggaagccgcg
    31861 accaataccg tgtccgcaat attcacgaac gacggagaag ccttctgctt cgacaaattt
    31921 ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt tttaccatgc gtagcgccag
    31981 gtacaggctt tcttgcgtga tgcggcacag gcgttcgccc atgatggtcg gcttaccgac
    32041 gataaacatt ttcgaggtat cgccgtggaa accatctttg attacggtaa catcaatgtt
    32101 aacgatatcg ccatctttca gcagcttagc atcgtccggg ataccgtggc acaccacttc
    32161 attaatagag atgcaaacgg atttcggata accgtgatag ccgaggcagg cagaaaccgc
    32221 gtgttgttca ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc
    32281 cggtttaaca tatggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg
    32341 cattttttcg atatcttctg aggtcttgat tgagatagcc attaattctg tccatcagcg
    32401 tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg
    32461 agaatcattc tgaatttcgc caaacgtgcc actgaacgtt ttctataata gaaaattcaa
    32521 cgtctgatgc tgtacacagc gccaacaatt attggtgtcc atgacgtatt tgtggtataa
    32581 agcgcgccgg acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac
    32641 tttgtgtaac aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat
    32701 atggggtacg tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt
    32761 ttccatgcgc gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa
    32821 cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga
    32881 gaaaactgta ccgatgttca acgaagctct ggctgaactg aacaagattg cttctcgcaa
    32941 aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc
    33001 tctgagctgc gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg
    33061 gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg
    33121 tactttcgac aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact
    33181 ggaaaacagc ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat
    33241 cgatgctgac cacgaacaca ttgctatcaa agaagcaaac aacctgggta ttccggtatt
    33301 tgctatcgtt gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga
    33361 cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg
    33421 ccgttctcag gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg
    33481 cttgataact cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg agacttcccg
    33541 ggagcttaca tcagtaagtg accgggatga gcgagcgaag acaacgcatc tgcggcgcga
    33601 aatatgaagg gggagagccc ttatagacca ggtagtacac gtttggttag ggggcctgca
    33661 tatggccccc tttttcactt ttatatctgt gcggtttaat gccgggcaga tcacatctcc
    33721 gaggatttta gaatggctga aattaccgca tccctggtaa aagagctgcg tgagcgtact
    33781 ggcgcaggca tgatggattg caaaaaagca ctgactgaag ctaacggcga catcgagctg
    33841 gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt
    33901 gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact acggcatcat tctggaagtt
    33961 aactgccaga ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt
    34021 ctggacgcag ctgttgctgg caaaatcact gacgttgaag ttctgaaagc acagttcgaa
    34081 gaagaacgtg ttgcgctggt agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct
    34141 gcgctggaag gcgacgttct gggttcttat cagcacggtg cgcgtatcgg cgttctggtt
    34201 gctgctaaag gcgctgacga agagctggtt aaacacatcg ctatgcacgt tgctgcaagc
    34261 aagccagaat tcatcaaacc ggaagacgta tccgctgaag tggtagaaaa agaataccag
    34321 gtacagctgg atatcgcgat gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt
    34381 gaaggccgca tgaagaaatt caccggcgaa gtttctctga ccggtcagcc gttcgttatg
    34441 gaaccggcaa aaactgttgg tcagctgctg aaagagcata acgctgaagt gactggcttc
    34501 atccgcttcg aagtaggtga aggcatcgag aaagttgaga ctgactttgc agcagaagtt
    34561 gctgcgatgt ccaagcagtc ttaattatca aaaaggagcc gcctgagggc ggcttctttt
    34621 tgtgcccatc ttgtaaattc agctaaccct tgtggggctg cgctgaaaag cgacgtacaa
    34681 tgtcgctagt attaattcat ttcaatcgtt gacagtctca ggaaagaaac atggctacca
    34741 atgcaaaacc cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca
    34801 ctgaaggctt cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac
    34861 tggttgaact gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg
    34921 ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg atgctggcga
    34981 ccgtaatgaa cggcctggca atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc
    35041 tgatgtccgc tattccattg aatggcgtgt gcgacagtta cagctgggca gaagctatca
    35101 gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg tacaggtaac ccgttcttta
    35161 ccaccgactc agcagcttgc ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag
    35221 caaccaaagt tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt
    35281 acgagcaact gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg
    35341 ccttcacgct ggctcgtgac cataaattac cgattcgtgt tttcaacatg aacaaaccgg
    35401 gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac tttaatcacg gaataattcc
    35461 cgtgatggat aaataagggt aagattccgc gtaagtatcg cgggggcgta agtctggtta
    35521 taaggcgtta ttgttgcagg cagtttggtc acggccagcg cgcagcaacc ggagcgtaca
    35581 aaagtacgtg aggatggcga gcactgcccg gggccaaaat ggcaaataaa atagcctaat
    35641 aatccagacg attacccgta atatgtttaa tcagggctat acttagcaca cttccactgt
    35701 gtgtgactgt ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata
    35761 tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca
    35821 gcaaaatacg cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtggaatatt
    35881 acggcacgcc gacgccgctg cgtcagctgg caagcgtaac ggtagaagat tcccgtactc
    35941 tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcct
    36001 ccgatctcgg cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc
    36061 tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc
    36121 gtgttgcagt acgtaatgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag
    36181 ataaagagat cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg
    36241 atgctgcaat caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt
    36301 tctgatttct tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg
    36361 ttttgctttt tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc
    36421 tcgaccggct cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc
    36481 cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa
    36541 ttctctcccc gctatgccgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg
    36601 ctacagcaac agggtagccg caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg
    36661 gcagcgcttg aggatgttga tcaggtgatg gcagccattg ttggcgctgc tgggctgtta
    36721 cctacgcttg ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg
    36781 gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg
    36841 gtcgatagcg aacataacgc catttttcag agtttaccgc aacctatcca gcataatctg
    36901 ggatacgctg accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc
    36961 cctttccgtg agacgccatt gcgcgatttg gcaacaatga cgccggatca agcctgccgt
    37021 catccgaact ggtcgatggg gcgtaaaatt tctgtcgatt cggctaccat gatgaacaaa
    37081 ggtctggaat acattgaagc gcgttggctg tttaacgcca gcgccagcca gatggaagtg
    37141 ctgattcacc cgcagtcagt gattcactca atggtgcgct atcaggacgg cagtgttctg
    37201 gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc acaccatggc atggccgaat
    37261 cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc
    37321 gcaccggatt atgatcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggc
    37381 caggcagcga cgacagcatt gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg
    37441 caacaaatcc gctttacgga tatcgctgcg ttgaatttat ccgtactgga aaaaatggat
    37501 atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc
    37561 gccagaaaag aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtcgcgc
    37621 tatttgttag cgtagggctt cagtgatata gtctgcgcca tctgatcgta agtagttggc
    37681 tttataaggt caggtatgcc gtggttttac acggcttttt tttgtatagg cttcagtatt
    37741 cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac
    37801 cacttagcga aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca
    37861 atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa
    37921 aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt
    37981 atgcctttag tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac
    38041 tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc
    38101 gtattattgg cgataccagt cgctttaact cgcgtttgca agaacgtatt cgtaaatctg
    38161 aagcgctaac agccgggaat accggtctga cgctgaatat tgcggcgaac tacggtggac
    38221 gttgggatat agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc
    38281 aaccagatca gatagatgaa gagatgctaa accagcatgt ctgtatgcat gaactggccc
    38341 ctgtagattt agtaattagg actggggggg agcatcgcat tagtaacttt ttgctttggc
    38401 aaattgccta tgccgaactt tactttacag atgttctctg gcccgatttc gatgaacaag
    38461 actttgaagg ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc
    38521 ccggtgatga aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg atatctgctt
    38581 ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca
    38641 ttgtaacgct ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta
    38701 ccactcgttc gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc
    38761 tttttctgtt gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt
    38821 gggcttcgct gggttggtgg attgtcgcgc tattgctggt gctgttttac ccaggttccg
    38881 cagcaatctg gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc
    38941 ccttcttctg gggcatgctg gcgttacggg cctggcacta tgacgagaat cattacagtg
    39001 gcgcaatatg gctgctctat gtcatgatcc tggtatgggg cgctgactcc ggcgcatata
    39061 tgtttggcaa attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct
    39121 ggcaaggctt tatcggtgga ctcgctactg cagcggtaat ctcatggggt tatggcatgt
    39181 gggcgaatct cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag
    39241 cctcagtgct cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca
    39301 gcggtcattt aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg
    39361 cggtaccggt ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa
    39421 tatgctgagt tttctctggg atttggcttc gttcatcgtt gcactggggg tacttatcac
    39481 cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt
    39541 ctcaataggg tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt
    39601 tatcgccctg atcccgttgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt
    39661 cgttccggaa ctccgccacc atgccttcaa taataaatct gtcggccaac gagcggcgat
    39721 tattgccgca ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt
    39781 tattattggt gtgcctggcg tacgtccggt ggttggcgaa atagcagcca attcgatagc
    39841 tgcggaagca caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc
    39901 tgattgggat gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat
    39961 tacagtagcg ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg
    40021 ggcgtttgag cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggcc
    40081 gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt
    40141 gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt
    40201 tgtgatgctt gtccgggata acccgggtaa atccttagcg ttagaaatcg aaaggcaggg
    40261 gagtcccttg tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg
    40321 ttttgtcggt attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca
    40381 gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct
    40441 gacggtcagt atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg
    40501 gccgatctct atcgccaagg gggctgggat gacagcggaa ctcggggttg tttattacct
    40561 gccgtttctt gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt
    40621 acttgacggg gggcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc
    40681 cgagcgggtt caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg
    40741 gcttgcactt ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata
    40801 acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac
    40861 ggtgctgaag ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt
    40921 ggtgcggccc tcctcagtat gccggtgcgc acaggcgaca cggttaatga tgaagatatc
    40981 agtaatacca ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt
    41041 gatggtgata cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc
    41101 tccggtaaca aatcggtgaa agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg
    41161 cgtgtgggcg aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac
    41221 ttctactaca gtgtcggtaa atatagcgcc agcgttaaag ctgtcgtgac cccgctgccg
    41281 cgcaaccgtg ttgacctaaa actggtgttc caggaaggtg tgtcagctga aatccagcaa
    41341 attaacattg ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg
    41401 cgtgacgaag tgccgtggtg gaacgtggta ggcgatcgta aataccagaa acagaaactg
    41461 gcgggcgacc ttgaaaccct gcgcagctac tatctggatc gcggttatgc ccgtttcaac
    41521 atcgactcta cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac
    41581 atcaccgaag gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg
    41641 cactccgctg aaattgagca gctgactaag atcgagccgg gtgagctgta taacggcacc
    41701 aaagtgacca agatggaaga tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat
    41761 ccgcgcgtac agtcgatgcc cgaaattaac gatgccgaca aaaccgttaa attacgtgtg
    41821 aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc
    41881 tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gctggggagc
    41941 gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat
    42001 accgataccc aacgtgttcc gggtagcccg gaccaggttg atgtcgtcta caaggtaaaa
    42061 gagcgcaaca ccggtagctt caactttggt attggttacg gtactgaaag tggcgtgagc
    42121 ttccaggctg gtgtgcaaca ggataactgg ttaggtacgg gttatgctgt tggtatcaac
    42181 gggaccaaaa acgattacca gacctatgct gaactgtcgg taaccaaccc gtacttcacc
    42241 gtagatggcg taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc
    42301 gacctgtccg actataccaa caagagttat ggtacagatg tgacgttggg cttcccgatc
    42361 aacgaatata actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg
    42421 cagccgcagg ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacctct
    42481 gatcaggata acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac
    42541 aagcttgacc gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg
    42601 accattcctg gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg
    42661 ccgatcgatg acgatcacaa atgggttgtt ctggggcgta cccgctgggg ttatggtgat
    42721 ggtttaggcg gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc
    42781 gtgcgtggct tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc
    42841 agtaattatg atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg
    42901 tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc
    42961 accccgacgc cgtttattag cgataagtat gctaactcgg ttcgtacttc cttcttctgg
    43021 gatatgggta ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac
    43081 tatagtgatc caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca
    43141 ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca
    43201 gaacagttcc agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt
    43261 ggtagtgtag cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg
    43321 caccctccgg tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagctgc
    43381 aggtctcggt ttagcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa
    43441 catgggcagc ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa
    43501 tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat
    43561 gaaaaagctg cagtccatga aagcgggcag cgatcgcact aagctggaaa aagacgtgat
    43621 ggctcagcgc cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg
    43681 ttccaacgaa gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc
    43741 caacagccag gatatcgatc tggttgttga tgcaaacgcc gttgcttaca acagcagcga
    43801 tgtaaaagac atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg
    43861 actggctgat ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat
    43921 caccggcgtt gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc
    43981 aaaataccgt gagcatttag gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga
    44041 tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg
    44101 catggcgcaa attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt
    44161 gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga
    44221 gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa
    44281 cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca
    44341 gatcggtcag aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta
    44401 tgccaacgat cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa ttattggcga
    44461 tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc gcgctggatg acactattat
    44521 tggcaatggc gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga
    44581 caatacggcg gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg
    44641 catgatcggc ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt
    44701 tacgggcatg ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat
    44761 tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga
    44821 catgagcaag cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat
    44881 cttttgttcg ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat
    44941 tattgtcgtt tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg
    45001 cagattgaag agattttaga acttctgccg caccgtttcc ctttcttact ggtggatcgc
    45061 gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag
    45121 ccattcttcc agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa
    45181 gcaatggcac aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt
    45241 gagctgtact atttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc
    45301 gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa
    45361 ggggttgctc tggtcgacgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc
    45421 cgggaggcct gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa
    45481 gagggcgcgt cgattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat
    45541 gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg ccatactaaa
    45601 attggccgcg ataatgagat ttatcagttc gcctccatcg gtgaagttaa ccaggatctg
    45661 aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc
    45721 gtcaccattc atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac
    45781 ttactgatga tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc
    45841 gccaacaacg caacgctggc gggtcacgta tcggtagacg acttcgcgat catcggcggc
    45901 atgaccgcag tccatcagtt ctgcatcatt ggtgcgcatg tgatggttgg cggttgctcc
    45961 ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc
    46021 ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc gcgaggcgat taccgctatc
    46081 cgcaatgcgt ataagctgat ttatcgcagc ggtaaaacgc tcgatgaagt gaaaccggaa
    46141 attgctgaac tggcggaaac gtatccggaa gtgaaagctt ttaccgattt ctttgcacgc
    46201 tcaacgcgcg gtctgattcg ttaatgactg aacagcgtcc attaacgatt gccctggtcg
    46261 ccggagaaac ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg
    46321 tgcccaacgc ccgctttgtt ggcgttgccg ggccacgaat gcaggctgaa ggctgcgaag
    46381 cctggtacga aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc
    46441 gtcgcttact gcatattcgt gccgatctga caaagcgttt tggtgaacta aagccagatg
    46501 tttttgttgg cattgatgcg cctgacttca atattaccct cgaaggtaac cttaaaaagc
    46561 agggtatcaa aaccattcat tatgtcagtc cgtccgtctg ggcgtggcga cagaaacgcg
    46621 ttttcaaaat aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt
    46681 tttatgacaa gtataacgtg ccgtgccgtt ttatcggtca cacgatggct gatgccatgc
    46741 cgttagatcc agataaaaac gccgcccgtg atgtgctggg tatccctcac gatgcccact
    46801 gtctggcatt gttgccgggc agccgtggcg cggaagtcga aatgcttagt gccgatttcc
    46861 tgaaaacggc ccagcttttg cgccagacat atccggatct cgagatcgtg gtgccgctgg
    46921 tgaatgccaa acgccgtgag cagtttgagc gtatcaaagc ggaagttgcg ccagacctgt
    46981 cggttcattt gctggatgga atgggccgtg aggcgatggt cgccagtgat gcggcgctac
    47041 tggcgtcggg tacggcagcc ctggagtgta tgctggcgaa atgcccaatg gtggtggggt
    47101 atcgcatgaa gcctttcacc ttctggttgg cgaagcgtct ggtgaaaact gattatgtct
    47161 cgctgccaaa tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg
    47221 agccgcaaaa actggctgcg gcgctgttac cgctgttggc gaacgggaaa accagccacg
    47281 cgatgcacga taccttccgt gaactgcatc agcagatccg ctgcaatgcc gatgagcagg
    47341 cggcacaagc cgttctggag ttagcacaat gatcgaattt gtctatccgc acacgcaact
    47401 ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc
    47461 ggtgattctc gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga
    47521 aaaacgtcgt ctggcgctct gtgaagagat caaagagaaa gcgttgagct ggagtctggg
    47581 ccgcgcggaa cctcacgaaa tcgatgaact gaatatttta catgcgacca tgctggcgat
    47641 gcagcgtgcc gttgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg
    47701 ctgcccgaaa ttaccgatgc cttcgatggc agtggtgaaa ggcgatagcc gcgtaccgga
    47761 aatcagtgcc gcgtctatcc tggcgaaagt gacgcgcgac gccgaaatgg cggcgctgga
    47821 tattgttttc ccgcaatatg gttttgtcca acacaaaggg tatccaaccg cttttcatct
    47881 ggaaaaactg gctgaacacg gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa
    47941 acgcgcactg ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa
    48001 gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg
    48061 cctggccaaa accgcaccgc tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc
    48121 gatcaccgat ttcaccaacc tttgcggtct ggtgaagttc tacggagcgg gacatggcgc
    48181 agggattaag cctatcgtcg gggcagattt taacgtccag tgcgacctgc tgggtgatga
    48241 gttaacccac ctgacggtac tggcggcgaa caataccggc tatcagaatc tgacgttgct
    48301 gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg
    48361 gcttatcgaa ttaaacgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg
    48421 acgcagtctt ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga
    48481 acacttcccg gatcgctatt ttctcgaact gattcgcacc ggcagaccgg acgaagaaag
    48541 ctatttgcac gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa
    48601 cgacgtgcgc tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca
    48661 cgacggcttt accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat
    48721 gcgtagcgaa gaggagatgt gcgagctgtt tgccgacatc cccgaagctc ttgccaacac
    48781 cgttgagatc gccaaacgtt gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca
    48841 gttcccgacc ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct
    48901 ggaagagcgt ctggcctttt tattccctga cgaggaagaa cgtgttaagc gccgcccgga
    48961 atatgacgaa cgtctggaga ctgaacttca ggttatcaac cagatgggct tcccgggcta
    49021 cttcctcatc gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtagggcc
    49081 aggccgtggc tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga
    49141 tccgctggaa tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc
    49201 tgacttcgac gttgacttct gtatggagaa acgcgatcag gttatcgagc acgtagcgga
    49261 catgtacggt cgtgatgcgg tatcgcagat tatcaccttc ggtacgatgg cggcgaaagc
    49321 ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc
    49381 gaaactgatc ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca
    49441 gctgccggaa atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa
    49501 actggaaggg gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcaccgac
    49561 caaaattacc gattttgcgc cgctttattg cgatgaagag ggcaaacatc cggtcaccca
    49621 gtttgataaa agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg
    49681 tacgctcacc atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg
    49741 cgagccgccg ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct
    49801 gcaacgctcg gaaaccactg cggtattcca gcttgaatcg cgcggcatga aggacctgat
    49861 caagcgtctg caacctgact gcttcgaaga tatgatcgca ctggtggcac tgttccgccc
    49921 cggtccgttg caatcaggga tggtggataa ctttatcgac cgtaaacatg gtcgtgaaga
    49981 gatctcctat ccggacgtgc agtggcagca tgaaagcttg aaaccggtac tggagccaac
    50041 ctacggcatc atcctgtatc aggaacaggt catgcagatt gcccaggtgc tttctggtta
    50101 taccctcggt ggcgcggata tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat
    50161 ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag aacggaatca acgccgaact
    50221 ggcgatgaaa atcttcgacc tggtggagaa attcgctggt tacggattta acaaatcgca
    50281 ctctgcggcc tatgctttgg tgtcatatca aacgttatgg ctgaaagcac actatcctgc
    50341 ggagtttatg gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct
    50401 ggtggatgag tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct
    50461 ttaccatttc cacgtcaacg acgacggcga aatcgtgtat ggtattggcg ccatcaaagg
    50521 ggtaggtgaa ggtccgattg aggccatcat cgaagcccgt aataaaggcg gttacttccg
    50581 cgaactgttt gatctttgcg cccgtaccga caccaaaaag ttaaaccggc gggtgctgga
    50641 aaaactgatc atgtccgggg cgtttgaccg tcttggacca caccgcgcgg cactgatgaa
    50701 ctcgctgggc gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca
    50761 ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag
    50821 ctgccaaccg tggccggagc aggtggtatt agatggggaa cgtgaaacgt taggcctgta
    50881 cctgaccgga caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt
    50941 aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc atcacggctg cggggctcgt
    51001 tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga
    51061 tgaccgttcc gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca
    51121 attgctggaa aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag
    51181 cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata
    51241 tgctcgcggg cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg
    51301 actccgtcag tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca
    51361 gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga
    51421 tcgtttatta aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga
    51481 ctaatacagg aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct
    51541 ggaagcgaaa atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa
    51601 catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta gaactgacgc gtaaaatctt
    51661 cgccgatctc ggtgcatggc agattgcgca actggcacgc catccacagc gtccttatac
    51721 cctggattac gttcgcctgg catttgatga atttgacgaa ctggctggtg accgtgcgta
    51781 tgcagacgat aaagctatcg tcggtggtat cgcccgtctc gatggtcgtc cggtgatgat
    51841 cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc
    51901 agcgccagaa ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat
    51961 gccaatcatc acctttatcg acaccccggg ggcttatcct ggcgtgggcg cagaagagcg
    52021 cggtcagtct gaagccattg cacgcaacct gcgtgaaatg tctcgcctta gcgtaccgac
    52081 tatttgtacc gttatcggtg aaggtggttc tggcggcgcg ctggcgattg gcgtgggcga
    52141 taaagtgaat atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc
    52201 cattctgtgg aagagcgctg ataaagcgcc gctggcggct gaagcgatgg gtatcatcgc
    52261 tccgcgtctg aaagaactaa aactgatcga ctccatcatc ccggaaccgc tgggtggtgc
    52321 tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc
    52381 cgatctcgac gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag
    52441 ctacggttac gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt
    52501 atcgccacgg tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac
    52561 atcattgcca ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg
    52621 gagtcggcgc tggtggtgca aggctttcag attatctggc cacaaaacag cgttgatttg
    52681 ctgaagttta tcgaacataa ccctcgaatt tgtggcgtga tttttgactg ggatgagtac
    52741 agtctcgatt tatgtagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc
    52801 atcaacaccc actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt
    52861 tttgaatatg cgcttgggca ggcggaagat atcgccattc gtatgcggca gtacaccaac
    52921 gaatatcttg ataacattac cccaccgttc acgaaagcct tgtttaccta cgtcaaagag
    52981 cggaagtaca ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg
    53041 gttggctgtc tgttttatga ttttttcggc gggaataccc tcaaggcgga cgtctctatt
    53101 tcggtcaccg agcttggttc gttgctcgac cacaccgggc cacacctgga agcggaagag
    53161 tacatcgcgc ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg
    53221 tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc
    53281 aattgtcata aatcgctggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg
    53341 aaaccgacgc gtaatgcgtt ggggattctg ggtgggatcc cgcgccgtga atttactcgc
    53401 gacagcatcg aagagaaagt cgctgccacc acgcaggcac aatggccggt tcatgcggtg
    53461 attaccaact ccacctatga tggcttgctc tacaacaccg actggatcaa acagacgctg
    53521 gatgtcccgt cgatccactt cgattccgcc tgggtgccgt acacccattt tcatccgatc
    53581 taccagggga aaagtggtat gagcggcgag cgtgttgcgg gaaaagtgat cttcgaaacg
    53641 caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt cgctgatcca cattaaaggc
    53701 gagtatgacg aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt
    53761 tatcccattg ttgcttcggt tgagacggcg gcggcgatgc tgcgtggtaa tccgggcaaa
    53821 cggctgatta accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggttg
    53881 cgggaagagt ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc
    53941 gaatgctggc ccgttgcgcc aggcgaacag tggcacggct ttaacgatgc ggatgccgat
    54001 catatgtttc tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc
    54061 aatatgagcg aggaggggat cccggcggcg ctggtggcaa aattcctcga cgaacgtggg
    54121 atcgtagtag agaaaaccgg cccttataac ctgctgtttc tctttagtat tggtatcgat
    54181 aaaaccaaag caatgggatt attgcgtggg ttgacggaat ttaaacgctc ttacgatctc
    54241 aacctgcgga tcaaaaatat gctacccgat ctctatgctg aagatcccga tttctaccgc
    54301 aatatgcgta ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt
    54361 cccggtttga tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag
    54421 gcatggcaga gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt
    54481 agagtatcgg caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgcctgga
    54541 gaaatgctga ccaaagagag ccgcacagta ctcgattttc tactgatgct ttgttccgta
    54601 gggcaacatt accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc
    54661 gtttaccgcg tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga
    54721 gtaacgtgct gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata
    54781 ttgcgattat tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt
    54841 tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc
    54901 ttaatgggca atatgtgatt gagcttttct catttccgtt cccgccggag cgccccagcc
    54961 gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt tgatgatatc gatgcggcag
    55021 tggcgcacct tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc
    55081 aaaaacgctt cacgttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt
    55141 aaggcttgtc atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc
    55201 accatcatga cactcacgct caatagacaa cttctcacct cacgccagat tctggtggcc
    55261 tttagcggcg ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa
    55321 aatccgggtg tcgctctgcg cgctattcat gtgcatcacg gtttaagtgc caatgccgat
    55381 acctgggtta cgcattgcga aaacgtctgc caacagtggc aggtgccgct ggtggtcgag
    55441 cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc aggcgcggca ggcgcgttat
    55501 caggcatttg cccgcacctt gttgcccggt gaagtgctgg tcaccgcgca acatctcgac
    55561 gatcaatgtg aaacttttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg
    55621 gctatggggg aagtctcgga gtttgccgga acacggctta tccgcccgtt gcttgcccgc
    55681 acgcgggggg aactggagca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa
    55741 agtaatcagg acgactcata cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg
    55801 cagcagcgtt ggccgcattt tgccgaagca acggcccgca gtgccgcact ttgtgctgaa
    55861 caagagagcc tgctggatga actgctggta gatgatttag cgcactgcca aacgccgcag
    55921 gggacgctgc agattgcgcc aatgatgacg atgagtgatg cccgtcgcgc ggcgattatc
    55981 cgtcgctggc tggcagggca gaatgcacca atgccttccc gcgacgcgct ggtgcgcatc
    56041 tggcaggaag tggcgctggc gcgggaagat gcctccccct gtttacgctt gggcgcgttt
    56101 gaaatccgac gctatcagtc gcaactgtgg tggattaaat ccgtcaccgg gcaaagcgaa
    56161 aacattgtgc cgtggcaggc atggcttcaa ccgctggaat taccggcggg gctgggaagt
    56221 gtacagctta ctgcgggagt cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg
    56281 cgtttcaaag cgccaggatt gctgcatatt gtcggtcgca acggcggacg taagctaaag
    56341 aaaatctggc aagagctggg cgtgccgccg tggctacgtg acaccacgcc actgctgttt
    56401 tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa
    56461 ggtgagaagg gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa
    56521 gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat tcgcttacca ccaccgtacc
    56581 gatttccggg tggctaaagc tggtaatttt atccagacgc agctcgcggg tttcgccagc
    56641 ggcctcgacg accagatact ccacattttt gcgggagact aaatcactgg cttttgcctg
    56701 caatttttcg ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc
    56761 aagattatcg taatcatcac aattgattgg ttgatacgta tcattcattg acataatcgc
    56821 tcaccagtaa gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt
    56881 cgtcggcagc catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc
    56941 ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca
    57001 catgccctcc tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata
    57061 aactctgttt cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg
    57121 cacgtattcc gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc
    57181 gacaatttat aaagcgacgg ttaatgtggc cgatctcgac cgtaaccagt ttctcgatgc
    57241 ctctctgacg ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcttgct
    57301 ggcgtggttg aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga
    57361 tgagccggaa gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg
    57421 gctgccggat gagcggcgga ttaagaaagc ctgcacccag gccgcaaaag tggcgctgtt
    57481 tacctataat agccgggcgg cgcaaatctg gtggcagcaa aatcagagca aatttgtgca
    57541 gtttgccaat ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt
    57601 tgccgatcgt accatgacgc tgcaggcaac gattcaggat ggcgtgatct ggttatcgga
    57661 tgataagaat aatctggaag tgaacttaac cgcctggcaa caaccttcat gattgtgatt
    57721 tcccgacatg ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc
    57781 gcgggcgggc agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg
    57841 gcgtccagcc tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc
    57901 agcagtgatg gcgtgattgt cattaaggca caggaatacc gcagtcagga actgaaccgc
    57961 gaagcggcgc tggcccggct ggtggcagtg attaaagatt taacaacaga acaaaaagcc
    58021 cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa
    58081 aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg gtcgggaata aaaagaagga
    58141 atggatggtg aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat
    58201 gggatgtaat aatcgggccg aagtcgatac gctttctccg gcgcaggctg ccgaactaaa
    58261 accgatgccg caaagttggc gcggcgtgct gccgtgtgcc gattgcgaag ggatcgaaac
    58321 ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc tcggtgctcg
    58381 tgaagaacct tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt
    58441 attaaccgac agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg cgctggagat
    58501 gctcgatcgt gaaggcaatc caattgaatc gcagttcaac tatacgctgg aaccggcaca
    58561 atccagttta cctatgacgc cgatgaccct gcgaggcatg tatttttata tggctgatgc
    58621 ggcgaccttc actgattgcg cgaccggaaa acgtttcatg gtagcgaata acgcagagct
    58681 ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa ccggtgttac tgtcagtaga
    58741 aggtcacttt acgcttgagg ctaatccgga tactggtgcg ccaaccaaag tattagcgcc
    58801 cgatacggcg ggtaaatttt acccgaataa ggattgcagt agtttggggc agtaacccgt
    58861 cttgagacag aaacaaacgc aaaattgcct gatgcgctgc gcttatcagg cctacagcat
    58921 ataatgcaac attttgaatt agcacgattt tgtaggccgg ataaggcgtt tacgccgcat
    58981 ccggcatttt gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc
    59041 ttgatataaa tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaat
    59101 gcagagacat cgccagtacg catatgcatc agatcggcag ggctaatcca gcccgattgc
    59161 gccagcgtaa gcggatgccc ggctttggca aatgcatcag tgacaaactc cgaacaaaac
    59221 cacgactttt tgtctccttc gcccacactg cttaactgcg ctttcgccag gccgctgacg
    59281 cactgttggc gaaaatctgc ggaaaaagga ttcagtgagc acatctggcg ggtcaccata
    59341 aatggaatga actcgacaat gccgcgatag ttatacccgc tatctttgat tttgttggcg
    59401 aacgcggtga tttctgtggc ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc
    59461 ttatcgctat gcttcatcgc ttttttaagg gaaacaatct ggacgccagc gcctgtcgct
    59521 tctgcaacgt tattttcacc cagatagatt gcaacgtgac tcacagagga agtgctgaag
    59581 acgcggattc caaatgaggt taccccaagg cttgaggaga acagcaaatc gccgggtttg
    59641 agatcaggtg ccgttatttc ttttattgat tgttcggtga aagagctttg atgctggaat
    59701 ttgacagccc atgtttttgc ctcggcatct actgctgtgg ctgatgggtc tggctggctg
    59761 atatcaacgg tacaagccga aagtaaaaga aagctgggga gaagcaggcg gcagtacgcc
    59821 tttggtttat ccattttata caatccatgt aaaaaagggc cctgaaattc aggacccttt
    59881 ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa
    59941 ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc
    60001 gcccagcaca atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg
    60061 acgctctttg cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag
    60121 tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac
    60181 ctggaacggc gcgatcgcgt caggccatac gatgccgcgt tcgtcgtagt tctgctcgat
    60241 cgccgcagct actacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttgatt
    60301 acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg
    60361 gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctgac catccgggct
    60421 tggatcgccc gccaccacgt tgcggatatc agcaatttcc ggggtagcga catcgcgatc
    60481 ccagttgata ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat
    60541 cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc
    60601 cggaccggct ttaaccacgg cacgaatttc ttcttcagtc gcaaaagtca gcgggctggc
    60661 aacctgcggc agtttttctg ctttaacttc gttcagctcg tggtcaccgc gcaccagcag
    60721 cgcaaccaac gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt
    60781 ctcaatcggc agattgaact gttcaaccag ttccgtgatg gttttcgcgt tcggcgtatc
    60841 aaccagcgtc atttcctggg tagccgcagc gcgcggttct ttcggcgcga tagcttctgc
    60901 cagttcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct
    60961 ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcctg
    61021 tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc
    61081 atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag
    61141 gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg
    61201 gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc
    61261 agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa
    61321 acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca aatcggctgg
    61381 ctgaaccacc ggcatcgaca cctcgattgc accggcgttg ttcatctctt cacgcacgat
    61441 gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc
    61501 cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc
    61561 aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca
    61621 gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaagtgatt tagtttacca
    61681 gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag cgcaaagact
    61741 tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca gacggcatag
    61801 gttttgcccg tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg
    61861 cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg cttttcgacc
    61921 tctgcggtaa aactcaccgc catttctgcc gctggcgcac tttgcgcata gctggcactg
    61981 gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac cggcgtacca
    62041 tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca aacaacctct
    62101 ttcagctcta ccagcgacat accaattggg ttagggcgga aagtagagcg cgtagcgaaa
    62161 acacccattc tggcattacc gccaagacga ggcggacgca cagtcggacg ccagccgcct
    62221 tccatcgttt gatgaaagac gaaaagtatc cataaatggc tgaacgcttc caggccgcga
    62281 acggcgtcgg cctggttgta gggagccatg agatgcagtt cgccgttggc gctttttacc
    62341 agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat aacgcctatt
    62401 tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg atacatacag
    62461 cctgacgata gcagcctggc gtaccgctgg taacttcgca gctatgcagt aataccgcat
    62521 tggctttcat tttcgaggcg ttgatttgca tccgcttacg tgcggttgga atgctcggcg
    62581 gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga tcgcggaacg
    62641 gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg cgcggcgctt
    62701 tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg
    62761 atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc ggtaaagcac
    62821 gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa
    62881 tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa cagcctaaaa
    62941 attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg taaacttcat
    63001 cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta tcttcacgcg
    63061 ttacgatcag gtttacgtac ggggagtctt tatcttcgac aaagataccg tctttcgctg
    63121 gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg atttgcgcgt
    63181 cgtccagaga tcgaggcagc tgcggtgctt ccagctcaac aattttcaga ttttttgggt
    63241 tctcaacaac atcaagaacg gtcggcagca ggccaacgcc atctttcagt ttgatcaagc
    63301 ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt ggcacggcaa
    63361 cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa ccagcaatcg
    63421 gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc agttgctgat
    63481 caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt
    63541 ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat ttgtctttcg
    63601 caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg ccgactttaa
    63661 tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat ccaatcaggg
    63721 ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc ttcaattatt
    63781 tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg cctgcgaact
    63841 gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc gtcgcgttat
    63901 agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg actgcaccac
    63961 ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc accagacccg
    64021 gcaacgcttc cggtaacagc actttacgga cgatctgcat tggcgtggca cccattgcgc
    64081 gggaagcttc aattaacccg gttgggatct ccagaagagc gttctcgacc atacgggcaa
    64141 taaacggcgc tgcgccaacg gttaacggaa caatcgctgc ctgcaaacca atcgatgtac
    64201 cgacaataac tcgggtaaac ggaatcatcc ataccagcaa gataatgaac gggatggaac
    64261 ggaaaatgtt cacaattgca gaaacggtac gatacagctt cgcgttagca ataatttgcc
    64321 ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaag ccaaaaaaac
    64381 cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc cacatcatcg
    64441 gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca ggcaatggcg
    64501 gcttgcgtat cttgttgtgt gccgtgcatt tcggtcagca tgatgccgaa cttaacgcca
    64561 ccggcgtaat ccatctgcgc gctaataatg ttgttgttga cgttgaaacg acgcgcggtt
    64621 tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag catcggcacg
    64681 cagtcagtaa atggctccgc ttgcagacgt tcctggtaat cttccgggat atccagatgc
    64741 agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt
    64801 accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc acaaatgcgc
    64861 ttcacaacgt ccatttcgtg agtgatcaac agaatcgtca aacccagacg gcggttgatg
    64921 tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca
    64981 tcacacagca atactttggg attgctggct aatgcacggg caatcgccac acgttgtttc
    65041 tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc aaccaatgac
    65101 agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag ctccagcggc
    65161 agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg gaaaatcata
    65221 ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcag ttcctggcca
    65281 tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg tataagcgta
    65341 ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg cacatgcagg
    65401 ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt ggtgatattc
    65461 gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtggca tctcgaatgc
    65521 ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt ctaaatcaat
    65581 caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag cgtcacatgc
    65641 cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct tgaccgtgat
    65701 ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga atttatcgac
    65761 ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc
    65821 aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac gctgaccgag
    65881 tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta ttattgcccg
    65941 catcatccgc agggtagtgt tgaagagttt cgccaggtct gcgattgccg caaaccacat
    66001 ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc ttcttatatg
    66061 gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg aacaaaagtg
    66121 ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga ttgggtgtta
    66181 aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc ataatgatta
    66241 aaagatgagc gattgaaata aaaatgcatt tttccacttg tcttcctgag atgactccct
    66301 ataatgcgcc tccatcgaca cggcggatgt gaatcacttc acacaaacag ccggttcggt
    66361 tgaagagaaa aatcctgaaa ttcagggttg actctgaaag aggaaagcgt aatatacgcc
    66421 acctcgcgac agtgcgctaa agcgcgtcgc aactgctctt taacaattta tcagacaatc
    66481 tgtgtgggca ctcgaagata cggattctta acgtcg
//
LOCUS       DAOFQD010000010        65196 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.010, whole genome shotgun sequence.
ACCESSION   DAOFQD010000010 DAOFQD010000000
VERSION     DAOFQD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.010"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..702)
                     /gene="lpxT"
                     /locus_tag="QBF46_000666"
     CDS             complement(<1..702)
                     /gene="lpxT"
                     /locus_tag="QBF46_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDQ6672895.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHF"
     gene            complement(741..1727)
                     /gene="yeiR"
                     /locus_tag="QBF46_000667"
     CDS             complement(741..1727)
                     /gene="yeiR"
                     /locus_tag="QBF46_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDQ6672896.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRTTPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1845..3311)
                     /locus_tag="QBF46_000668"
     CDS             complement(1845..3311)
                     /locus_tag="QBF46_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDQ6672897.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(3534..4106)
                     /gene="yeiP"
                     /locus_tag="QBF46_000669"
     CDS             complement(3534..4106)
                     /gene="yeiP"
                     /locus_tag="QBF46_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDQ6672898.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4261..4515
                     /locus_tag="QBF46_000670"
     CDS             4261..4515
                     /locus_tag="QBF46_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDQ6672899.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(4512..5693)
                     /gene="setB"
                     /locus_tag="QBF46_000671"
     CDS             complement(4512..5693)
                     /gene="setB"
                     /locus_tag="QBF46_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDQ6672900.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6061..7191
                     /gene="fruB"
                     /locus_tag="QBF46_000672"
     CDS             6061..7191
                     /gene="fruB"
                     /locus_tag="QBF46_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDQ6672901.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVHAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPLAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7191..8129
                     /gene="fruK"
                     /locus_tag="QBF46_000673"
     CDS             7191..8129
                     /gene="fruK"
                     /locus_tag="QBF46_000673"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDQ6672902.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8146..9837
                     /gene="fruA"
                     /locus_tag="QBF46_000674"
     CDS             8146..9837
                     /gene="fruA"
                     /locus_tag="QBF46_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDQ6672903.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            10294..11235
                     /locus_tag="QBF46_000675"
     CDS             10294..11235
                     /locus_tag="QBF46_000675"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDQ6672904.1"
                     /translation="MREKNYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            11223..12161
                     /gene="psuG"
                     /locus_tag="QBF46_000676"
     CDS             11223..12161
                     /gene="psuG"
                     /locus_tag="QBF46_000676"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDQ6672905.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            12255..13505
                     /locus_tag="QBF46_000677"
     CDS             12255..13505
                     /locus_tag="QBF46_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDQ6672906.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13576..14274)
                     /gene="yeiL"
                     /locus_tag="QBF46_000678"
     CDS             complement(13576..14274)
                     /gene="yeiL"
                     /locus_tag="QBF46_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDQ6672907.1"
                     /translation="MKEIHNNDLKQQLMSESTFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALSMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            14404..15345
                     /gene="rihB"
                     /locus_tag="QBF46_000679"
     CDS             14404..15345
                     /gene="rihB"
                     /locus_tag="QBF46_000679"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDQ6672908.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNNHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            15445..16695
                     /locus_tag="QBF46_000680"
     CDS             15445..16695
                     /locus_tag="QBF46_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDQ6672909.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16751..17839)
                     /locus_tag="QBF46_000681"
     CDS             complement(16751..17839)
                     /locus_tag="QBF46_000681"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDQ6672910.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17843..18700)
                     /gene="nfo"
                     /locus_tag="QBF46_000682"
     CDS             complement(17843..18700)
                     /gene="nfo"
                     /locus_tag="QBF46_000682"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDQ6672911.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18774..19823)
                     /locus_tag="QBF46_000683"
     CDS             complement(18774..19823)
                     /locus_tag="QBF46_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDQ6672912.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            19922..20803
                     /locus_tag="QBF46_000684"
     CDS             19922..20803
                     /locus_tag="QBF46_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6672913.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            21008..22477
                     /gene="lysP"
                     /locus_tag="QBF46_000685"
     CDS             21008..22477
                     /gene="lysP"
                     /locus_tag="QBF46_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDQ6672914.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22771..24762
                     /gene="cirA"
                     /locus_tag="QBF46_000686"
     CDS             22771..24762
                     /gene="cirA"
                     /locus_tag="QBF46_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDQ6672915.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHYKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(24794..25630)
                     /gene="yeiG"
                     /locus_tag="QBF46_000687"
     CDS             complement(24794..25630)
                     /gene="yeiG"
                     /locus_tag="QBF46_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDQ6672916.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25888..26556
                     /gene="folE"
                     /locus_tag="QBF46_000688"
     CDS             25888..26556
                     /gene="folE"
                     /locus_tag="QBF46_000688"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDQ6672917.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            26573..27730
                     /locus_tag="QBF46_000689"
     CDS             26573..27730
                     /locus_tag="QBF46_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDQ6672918.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            27872..28912
                     /gene="galS"
                     /locus_tag="QBF46_000690"
     CDS             27872..28912
                     /gene="galS"
                     /locus_tag="QBF46_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDQ6672919.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            29192..30190
                     /gene="mglB"
                     /locus_tag="QBF46_000691"
     CDS             29192..30190
                     /gene="mglB"
                     /locus_tag="QBF46_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDQ6672920.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            30251..31771
                     /gene="mglA"
                     /locus_tag="QBF46_000692"
     CDS             30251..31771
                     /gene="mglA"
                     /locus_tag="QBF46_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDQ6672921.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31787..32797
                     /gene="mglC"
                     /locus_tag="QBF46_000693"
     CDS             31787..32797
                     /gene="mglC"
                     /locus_tag="QBF46_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDQ6672922.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(32868..34103)
                     /gene="preA"
                     /locus_tag="QBF46_000694"
     CDS             complement(32868..34103)
                     /gene="preA"
                     /locus_tag="QBF46_000694"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDQ6672923.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(34097..35335)
                     /gene="preT"
                     /locus_tag="QBF46_000695"
     CDS             complement(34097..35335)
                     /gene="preT"
                     /locus_tag="QBF46_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDQ6672924.1"
                     /translation="MPQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MVADKIILAVGQHARLDAFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(35529..35768)
                     /locus_tag="QBF46_000696"
     CDS             complement(35529..35768)
                     /locus_tag="QBF46_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDQ6672925.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(35771..36490)
                     /gene="sanA"
                     /locus_tag="QBF46_000697"
     CDS             complement(35771..36490)
                     /gene="sanA"
                     /locus_tag="QBF46_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDQ6672926.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36640..37524)
                     /gene="cdd"
                     /locus_tag="QBF46_000698"
     CDS             complement(36640..37524)
                     /gene="cdd"
                     /locus_tag="QBF46_000698"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDQ6672927.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(37654..38349)
                     /locus_tag="QBF46_000699"
     CDS             complement(37654..38349)
                     /locus_tag="QBF46_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDQ6672928.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(38346..38744)
                     /locus_tag="QBF46_000700"
     CDS             complement(38346..38744)
                     /locus_tag="QBF46_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDQ6672929.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            38983..39933
                     /gene="dusC"
                     /locus_tag="QBF46_000701"
     CDS             38983..39933
                     /gene="dusC"
                     /locus_tag="QBF46_000701"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDQ6672930.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATDLVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(40321..40404)
                     /gene="yohP"
                     /locus_tag="QBF46_000702"
     CDS             complement(40321..40404)
                     /gene="yohP"
                     /locus_tag="QBF46_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDQ6672931.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            40628..42064
                     /gene="mdtQ"
                     /locus_tag="QBF46_000703"
     CDS             40628..42064
                     /gene="mdtQ"
                     /locus_tag="QBF46_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDQ6672932.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAVQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            42117..42878
                     /locus_tag="QBF46_000704"
     CDS             42117..42878
                     /locus_tag="QBF46_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6672933.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIATRQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(43008..43586)
                     /locus_tag="QBF46_000705"
     CDS             complement(43008..43586)
                     /locus_tag="QBF46_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6672934.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHTLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            43756..44343
                     /locus_tag="QBF46_000706"
     CDS             43756..44343
                     /locus_tag="QBF46_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDQ6672935.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            44517..45449
                     /gene="pbpG"
                     /locus_tag="QBF46_000707"
     CDS             44517..45449
                     /gene="pbpG"
                     /locus_tag="QBF46_000707"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDQ6672936.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(45488..47203)
                     /gene="dld"
                     /locus_tag="QBF46_000708"
     CDS             complement(45488..47203)
                     /gene="dld"
                     /locus_tag="QBF46_000708"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDQ6672937.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFNQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            47399..49696
                     /gene="bglX"
                     /locus_tag="QBF46_000709"
     CDS             47399..49696
                     /gene="bglX"
                     /locus_tag="QBF46_000709"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDQ6672938.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVNELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEARKVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            49948..50865
                     /gene="osmF"
                     /locus_tag="QBF46_000710"
     CDS             49948..50865
                     /gene="osmF"
                     /locus_tag="QBF46_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDQ6672939.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGATPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            50872..52029
                     /gene="yehY"
                     /locus_tag="QBF46_000711"
     CDS             50872..52029
                     /gene="yehY"
                     /locus_tag="QBF46_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDQ6672940.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGARRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            52022..52948
                     /gene="yehX"
                     /locus_tag="QBF46_000712"
     CDS             52022..52948
                     /gene="yehX"
                     /locus_tag="QBF46_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDQ6672941.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERADGEALAEEMTLRDALS
                     LFVARGCEVLPVVNMQGQPCGTLHFQDLLVEA"
     gene            52953..53684
                     /gene="yehW"
                     /locus_tag="QBF46_000713"
     CDS             52953..53684
                     /gene="yehW"
                     /locus_tag="QBF46_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDQ6672942.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(53665..53772)
                     /locus_tag="QBF46_000714"
     CDS             complement(53665..53772)
                     /locus_tag="QBF46_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDQ6672943.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(53832..54563)
                     /gene="mlrA"
                     /locus_tag="QBF46_000715"
     CDS             complement(53832..54563)
                     /gene="mlrA"
                     /locus_tag="QBF46_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDQ6672944.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            54785..56470
                     /gene="btsS"
                     /locus_tag="QBF46_000716"
     CDS             54785..56470
                     /gene="btsS"
                     /locus_tag="QBF46_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDQ6672945.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            56467..57186
                     /gene="yehT"
                     /locus_tag="QBF46_000717"
     CDS             56467..57186
                     /gene="yehT"
                     /locus_tag="QBF46_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDQ6672946.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            57233..57703
                     /locus_tag="QBF46_000718"
     CDS             57233..57703
                     /locus_tag="QBF46_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDQ6672947.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(57744..58205)
                     /locus_tag="QBF46_000719"
     CDS             complement(57744..58205)
                     /locus_tag="QBF46_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDQ6672948.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARALEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(58330..60333)
                     /locus_tag="QBF46_000720"
     CDS             complement(58330..60333)
                     /locus_tag="QBF46_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDQ6672949.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPAEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHIVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLCQLAALNSRLNCAHEMAQRDSVGEIPPMPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVYLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(60330..61466)
                     /locus_tag="QBF46_000721"
     CDS             complement(60330..61466)
                     /locus_tag="QBF46_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ6672950.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDGNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(61459..63738)
                     /locus_tag="QBF46_000722"
     CDS             complement(61459..63738)
                     /locus_tag="QBF46_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672951.1"
                     /translation="MSEPLIVGIRHQSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRLREAFMARWIGWAMQQNNGDVLVVCGGWHAPVLAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHNGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALMMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DDDFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAYH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(63749..64837)
                     /locus_tag="QBF46_000723"
     CDS             complement(63749..64837)
                     /locus_tag="QBF46_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDQ6672952.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT        15728 a        16247 c        17339 g        15882 t
ORIGIN      
        1 gaaatgtttg ttttttcctg gtagtgattt gtcaaaaaag gtgattaatc gatcacttaa
       61 tggcgtcagc aacacccagg gcaaaccgat caatatcacg gtcatcgaac cgacaatgat
      121 gtcagtaaac cagtgtgcgc caatcattac tctgggaaat gcaaaaacca caaaaataat
      181 aagggcgata aggcctgcaa ctttgccgaa ataacgccac atgaatgccg aaaaaataag
      241 cagcatcatg ccgtgatcgc cgggaaagct atctcgtgag gcatctttcg tgggaacaga
      301 gagcagttcg ctgacgcggt taatatcggt aaaagtcaat gttgggctgg cccgttttac
      361 aggaattaat gcctgaccca gctggtttaa taccactgca gttaatagca tgaccagacc
      421 aataatcacg atacgtcgtc tgccaggggc gttttctttc agccagaaac tcagcatcaa
      481 catacccatc gccagcagtg aacaaccatc gaaggcgcga ttgttggtca atgcaaccag
      541 ccacaaaaag gccttacttt cgaccagttt ctgattaaag aaataaaaaa tatccgcatc
      601 aatcggcaac cagaatccat gattaacggg gatataccag gaaagaaaca gcgcgaggcc
      661 gacaatattc aacaacacta tttgcggcaa atttttaatc atattttctc taattatctt
      721 actgaaaaac gcaggcaacc ttacgcggta gtcgctaaac gaagcttcaa cagcgcgctc
      781 tgcaaggcat tccagtcagc ttcgctgctg gaaatcagct caatacggct gtccggtggc
      841 gcaacgtttt gcgtttcaat gtgcaggtca tcgccctgac ggttgattcg caccagccct
      901 tcgggaatac gcagcacgcc tttgacgcgt tccaccggtg caagtcgcgc ccattccaga
      961 atgccaatgg tgtcgaatac cgtatcagcg tcgaatatcc agccgcaggc ctgatatcct
     1021 tgcccgctgt tcagactgcg acgccagcgt tgatgctctg gcaggcttaa cgctgctaac
     1081 ccttttttca cgacatgctg atgagaatgc gcggcgctgg cgggcaactc ggctaaattg
     1141 cgacgcggca aatccagaag atgaccgtca actttcccat gctcactgtg aattaattgt
     1201 cgatcgccac cattttgctg ccaccaacgc tgtagcgctt gctcactttc gggcgtcgta
     1261 cggtcggatt tattggcgac aatgatgtct gcggcagcca gctggtcacg gaagttttca
     1321 ttgctggcgc ttttttcgtc cagcagcaag cgcggatcga gaatgcacaa ggtggcgcgc
     1381 agatctatcc acggttcata gactggtgcg gttaacagat cgaggatctg tttcggatgg
     1441 cccagcccgg tcggctctat caacaagcgg tctggttttc cctgacgcag taaggtattc
     1501 aaccctacct gcatgggtaa accattaacg cagcacatgc agccgccggg gatctctttc
     1561 agcaatgcgc cgctatcggc gagcaaagca ccatcaattc cgacttcccc aaattcatta
     1621 accagtaccg cccatttttc gttgggatct ttatgggcta acagatgaag aatcgacgtg
     1681 gttttcccgc tgccgagaaa accggtgatg aggttggtcc tggtcacaac ggctccgcaa
     1741 tactctggta agttaactga gcgttaatcc tggctaaaaa aggcagaaaa gagaagggga
     1801 gagcggacga aggtgagctt catccgcttt aaccgcagaa atcgttaaat tttgagggtg
     1861 tttaatagcg cctgatgtgc gccgaattgt acgatttgtt gccaggcttg ttcgatggcc
     1921 tgcacaaaat gcgggttatc tggcagatca tcaccgaaaa cttcacgcag ggaaagcagg
     1981 gcggttacgc gttgttcact gctgctgccc gcaacaagtt cgcgaatttt atcgctaagc
     2041 ggatcgcgaa catcaatggc atttccggca tcatcaacgc cgctgacgta acgcatccag
     2101 cctgcaacgc ctaatgccag caacgaccag tccgtttcgc gcccctgatg tatgcgaata
     2161 cctgccagca tgcgttgcgg taatttctgg ctgccatcca tcgcgatttg ccaggtctta
     2221 tgtttcagcg ccggattagc aaaacgtgca attaacttat ccgcatattg tgttaaatcg
     2281 acatctttaa tttgcagtgt cggcgcttgc tcatccagca ttaatgttct ggcggcatgg
     2341 cgaaatgcgc gatcctgcat gcaatcactg atatgggcga atcctgagag gtaacccaga
     2401 taagcgagaa aagagtggct gccattaagc atccgcagtt tcatctcttc ccatggcagg
     2461 acatcattca ccatttgtac acctgcgact tcccaggcag gacgcccagc gacgaagtta
     2521 tcttccacca cccactggat aaacggttcg cagctaatcg cgcagggatc attcaccccc
     2581 agatgctggc tgatttccac cagtgattcg tcggttgcag ccggaacaat gcggtcgacc
     2641 atggttcccg gaaaactgac gtgctctttt atccacccgg cgagttctgg cgaacgtttt
     2701 tctgccattc ccagcaccgc gtttttcacc acatgaccat tgtcgggaat attgtcgcag
     2761 gagagcacgg taaacggtgt aaggccgcgc tcacggcggc gtttcagtgc ttcgacgaga
     2821 atacccggtg cggagtgagg ttcttcaggg gtttgtagat cgtgaataat ccgcggatta
     2881 ctggtgtcga gtgcaccggt ggccgggtca atacaatagc ctttttcggt aatcgtcagg
     2941 gaaacaattg ccacctgtgg ctcgcaaaat ttctcaataa ttgccgctaa ggaatccagt
     3001 tttgcattaa ggcattcgtg aacggcaccg acaattatca cctgattgcc gtccgcacct
     3061 ttctccagca cggtatataa atggttctgt gcgcggagct ggctcatcag ttgatcaccg
     3121 ctgaacaagc tgatttcaca gatcccccag tcgccgccct gggcattcag cacacgatcg
     3181 gtcagtaatg cctgatgtgc gcggtgaaag gcgccaaagc caaaatgaac gatacgtgat
     3241 tgcaactgct ggcgatcgta gcgtggagcg tgtacatgat gtgggagcgt aacggaggca
     3301 attgtattca taggagtcac gtcttagtaa cgattaatta acaatgacag tgtaaagtta
     3361 tatgacagca attgaaattg gtctggattg ttaatgcgat gaagtgtgag ggcgatcaat
     3421 caacgagtga atattgcggg cgtaaggtgt tcaggaagcg caaaatagaa gattggtata
     3481 ataaattaaa aaagggacag cctgagctat cccttttctg catgcggctg aagttagtca
     3541 gcacgcccca tataacggcg ttcttcgata tggatacgaa ttttttcgcc tgggcttaag
     3601 tattctggta cctgaatcac cagaccagtg ctcaatgtcg ccggtttgtt acgggcgctg
     3661 gcagatgccc ctttgatgcc tggtgcagtt tcaacgattt ccagatcaac ggtctgcggc
     3721 agctcaagcg ccagcagttg accgtcccag gtcagcacct gcatgtccgg catgccgccc
     3781 tctggcataa acagcaactc ttcttcaatc tgatctttgg tgaaggtata cggggtatag
     3841 tcttctttat ccataaagac atattcgttg ccatcgacat aggagaagtc aacgtaacgg
     3901 cgggtcagcg tcacggtgtc aacgatatca tcacctttga aacgctcttc tactttcagc
     3961 ccggtacgga catcagaaaa acgcattttg tacagcgtag cggcaccgcg ggcagtgggc
     4021 gactgaatat caatatcctt aaccagcagc agtttgccgt tgtaattcag taccatacct
     4081 tttttaattt cgttcgctct tggcatcgaa aaattcctgt agtcgaaaat gtcaaaaata
     4141 tcgcgacaaa gtactcgcga cgggcctttc gggcaagtgg tattcgcact tttgctggtg
     4201 caaaaaaggt ggtactgtgc gcgctcatca atccggtggt taaccttaag agaacaacgt
     4261 atggaatgcc gtccgggttg cggggcgtgt tgcactgccc cttcaatttc cagccctatt
     4321 cccggtatgc cagatggcaa gcccgccaat acgccctgca ttcagcttga tgaacagcaa
     4381 cgctgtaaaa ttttcacctc gccactgcga ccgaaagtct gtgcaggctt acaagccagc
     4441 gctgaaatgt gcggtaactc gcgtcagcag gccatgacct ggcttatcga tctggaaatg
     4501 ctgaccgcgc cttaaacatc tttaatccgc agtaagcaaa acagagtggc gataatcatc
     4561 accatcgcaa accagaacac agcgtgataa ttccagatct cggcgacgat gcccgccact
     4621 gatcctgcga tgatccagcc cacgcgcgac gtgttggtat agagcgtggt ggctgaaccc
     4681 gcctgaccgg gcatcagatc ctgaaaatag agcatcccga tgccgcccag aatgccaata
     4741 aaaatagcat ttagcagctg caagcccaac agaatgacag gtgaatgcgc catcagcatt
     4801 cctgcgtaaa aacagacgcc acccacggca gcaacgcgca ttaagaaacg cttacccaga
     4861 cgtttggcga aatatccggc aatcaacatc gtcgggattt ccagcccggc ggcggtcccc
     4921 atcatcacac cggccagttt ctcgggaaga tgcagttcgt tgataataaa tagcggcatg
     4981 ttgatgatgt acaggctgtt cgagccccac atcaatgtac aaatgacaaa cagcagcagc
     5041 gtatcgcgac ggttacggcg cggcgcttca accgtgccgg tcgccagcgg aagctctttt
     5101 tgcatcgacg gtaaaaacag ccacaccatc acgccgcaaa caataaacgc tactgctgcg
     5161 ctcagataca ttaccgtaaa gctgaaaccc atcgctaagg cataagccag cggtgggcca
     5221 atgacccatg ccagtgaaac ctgagcgcgt aaaaaagagc tgaacatcac cgcttcacgt
     5281 ccggttttgt cggcatgttc acgggcaagg gcaaacattt gcgggttagc ggtcgagcca
     5341 aaactgctaa gaaagacgcc aacgaatagc aaaacaaagt agttgcgatt ccaggcaaaa
     5401 agggtgcagg ccagcacgcc taacaggcag caaaagacaa tcagcgattt gcgatcgccg
     5461 cgcttatcag agcgcccggc gagaaactga cttaccagaa tcccaatgac agcgctgccg
     5521 gtaaagaaga atcccaccat cgccggacgg gcatgtactt catcggtaag aaaaatactg
     5581 agtgtcgggg tttgcagagc gcccgcaata ccggtgagaa aggcaacgat taaaaacgcc
     5641 gtcgaggtca ggtcaaacga tttcgcgctg gagactgcgg gggagttatg catgctggtc
     5701 gatcactgga agaggaagac gcggagttta cgctgcttgt cagaaaataa acagacagcg
     5761 aaatcaaaag cgccacgaat aatcaaaatg ccatttcact gttgctgatt ggctgctgac
     5821 gctgctgaaa tgaaattgct gataacggat tttcccatca gcaattagga aaaatggcaa
     5881 aaaattgtgc agcacatcaa acttttgctc ataactttac ggctttcctt gcgtgctgaa
     5941 acagaagtat tatgctttct tgaaacgttt cagcgcgatc ttgtctttaa ccctaagcca
     6001 ggttggcgct ttttttctca tagaggctga atcgtttcaa ttcagcaaga gaggagaact
     6061 atgttccagt tatccgtaca ggacatccat ccgggcgaaa aggccggaga caaagaagag
     6121 gcgattcgcc aggtcgctgc ggcgctggtg catgccggta atgtagcaga aggctacgtc
     6181 aatggcatgc tggcgcgcga gcagcaaacc tcaacgttcc tcggcaatgg tattgctatt
     6241 ccacacggca ccaccgacac ccgcgatcag gtgctgaaaa cgggcgttca ggtatttcag
     6301 ttcccggaag gcgtcacctg gggtgacggt caggtagcgt acgtggcgat cggtattgct
     6361 gccagctcgg atgaacatct gggcctgcta cgccagctga cccacgtact gagcgatgat
     6421 tccgttgctg aacaactgaa gtcagcaaca acagcagaag aacttcgcgc attgctgatg
     6481 ggcgaaaagc agagtgagca gctgaagctc gacaacgaaa tgctgacgct ggatatcgtc
     6541 gccagcgatc tgctgactct tcaggcgctg aacgctgcgc gtctgaaaga ggcgggggca
     6601 gttgacgcca ctttcgtcac caaagccatc aatgaacaac cgctgaacct cggacagggc
     6661 atctggctga gcgatagcgc cgaaggcaat ctgcgtagcg cgattgcggt aagccgtgcg
     6721 gcaaatgctt ttgatgtgga cggcgaaacg gcagccatgc tggtgagtgt ggcgatgaat
     6781 gacgatcagc ccctcgcggt tcttaagcgt ctcgctgatt tgttgctcga caataaagct
     6841 gaccgcttgc tgaaagcgga tgcggcaacg ttgctggcgc tgctgaccag cgatgatgcg
     6901 ccgaccgacg acgtgttaag cgcggagttt gtggtgcgca atgaacacgg cctgcatgct
     6961 cgtccaggta ccatgctggt caataccatt aaacaattta acagtgatat taccgtgaca
     7021 aaccttgatg gcaccggcaa accggcaaac ggacgtagtc tgatgaaagt tgtggcactt
     7081 ggcgttaaga aaggtcatcg cctacgcttt accgcccagg gtgcagatgc tgaacaggcg
     7141 ctgaaagcaa tcggcgacgc tatcgctgct ggtcttgggg agggcgcata atgagcagac
     7201 gtgttgctac tatcaccctt aatccggctt atgaccttgt tggtttctgc ccggaaattg
     7261 aacgcggcga agtgaacctg gtgaaaacca ccggtctgca tgcggcgggt aaaggcatca
     7321 acgtggccaa agtattaaaa gacctgggaa ttgatgtcac cgttggcggc ttcctcggta
     7381 aagacaatca ggatggtttt cagcaactgt tcagcgagct gggcattgcc aaccgtttcc
     7441 aggttgtaca ggggcgcact cgaattaacg ttaagctgac ggaaaaagac ggcgaagtga
     7501 ccgacttcaa cttctcgggt tttgaagtca cccccgccga ctgggaacgc tttgtgactg
     7561 attctctgag ctggctcggt cagttcgata tggtctgtgt cagcggaagc ttaccgtcag
     7621 gcgtcagccc ggaagcgttc accgactgga tgactcgcct gcgtagtcag tgtccttgca
     7681 ttatctttga tagtagccgt gaagcgttag tagcaggttt gaaagcggca ccgtggctgg
     7741 tgaaacctaa ccgccgcgag ctggaaatct gggcaggccg taaactgcct gaaatgaaag
     7801 atgtgattga agctgcgcat gcgctgcgtg aacaaggtat cgcgcatgtt gttatttcac
     7861 tgggtgccga aggcgcgctt tgggttaatg cctccggcga atggatcgcc aaaccaccgt
     7921 cagtcgatgt cgtaagcacc gttggcgcag gggattctat ggttggtggc ctgatttatg
     7981 gcttgctgat gcgtgaatcc agtgaacaca cactgcgtct ggcgacagct gttgcagccc
     8041 tggcggtaag tcaaagcaat gtgggtatta ccgatcgtcc gcagttggcc gcaatgatgg
     8101 cgcgcgtcga cttacaacct tttaactgac agcaggagag gcataatgaa aacgctgctg
     8161 attattgacg ctaatctcgg tcaggcacgc gcctatatgg cgaagaccct gctgggcgcg
     8221 gcggcgcgaa aagcaaaact ggaaatcatc gacaatccga acgacgcaga aatggcgatt
     8281 gttctcggtg attccatccc gaacgacagc gcgctgaacg gtaaaaatgt ctggctgggt
     8341 gatatttccc gggcagttgc gcaccctgag ctgttcctga gtgaagccaa aggccatgcg
     8401 aaaccttaca ctgcgccggt cactgcgaca gcaccggttg ccgccagcgg tccgaaacgc
     8461 gtagttgcgg tgactgcttg cccgactggc gtagcacaca cctttatggc ggctgaagcc
     8521 attgaaaccg aagcgaaaaa acgtggctgg tgggtgaaag ttgaaacccg tggttctgtt
     8581 ggcgcgggta atgcaatcac tcccgaggaa gtagccgcag cggatctggt gattgtggcg
     8641 gcagatatcg aagtggatct ggcgaaattt gctggtaaac cgatgtatcg tacctctacc
     8701 ggtctggcgc tgaagaaaac cgcgcaggaa ctggataaag cggttgctga agcaacgccg
     8761 tatgaaccgg cgggcaaagc tcaaacggcg accactgaag gtaagaaaga gagtgcaggc
     8821 gcttatcgtc acttgctaac gggcgtctct tacatgctgc cgatggtcgt tgctggtggt
     8881 ctgtgtatcg cgctttcttt tgcttttggt atcgaagcgt ttaaagagcc gggtacgttg
     8941 gcagcggcgc tgatgcagat tggtggtggt tcagcctttg cgctgatggt gccggtactg
     9001 gcaggttata ttgccttttc cattgccgat cgtccgggcc tcacgccggg tctgattggc
     9061 ggtatgctgg cggtcagcac cggttctggc ttcattggcg gtattattgc gggcttcctg
     9121 gctggttaca ttgcgaagtt aatcagtacg caactgaaac tgccacagag tatggaggcg
     9181 ctgaaaccaa tcctgatcat tccgctaatt tccagtctgg tggtcggtct ggcgatgatc
     9241 tacctgatcg gtaaaccggt tgctggcatt ctcgaagggc tgactcactg gctgcagacc
     9301 atggggactg cgaatgcggt tctgctgggg gcgatcctcg gtggcatgat gtgtactgac
     9361 atgggcggtc cggtaaacaa agcagcgtac gcattcggtg tgggtctgct gagtactcaa
     9421 acctatggcc cgatggcggc gattatggcg gcaggtatgg tgccaccgct ggcaatgggt
     9481 ctggcaacaa tggtggcgcg tcgcaaattc gacaaagcgc agcaggaagg tggcaaagcc
     9541 gctctggtat tgggactgtg cttcatttcg gaaggtgcaa ttccgtttgc tgctcgtgat
     9601 ccgatgcgtg tgctgccgtg ctgtatcgtg ggtggtgcgc tgactggcgc aatctcaatg
     9661 gcgattggtg cgaaactgat ggcaccacac ggtggtctgt ttgttctgct gatccctggc
     9721 gctattacgc cggtactggg ttacctggta gcaattattg ccggtacgct ggtggcgggt
     9781 ttggcctatg ccttcctgaa acgtccggaa acacaaatag tagaaaaaaa cgcataattt
     9841 atcttcatta tcggggatga gacatctctg ccccgatgat gacaaacgca aaattgcctg
     9901 atgcgctatg cttatcagac ctacatgaac cctgcaatat attgaatttt tgcgcttttg
     9961 taggtcggat aaggcgttta cgccacatcc aggatgaaca aagcgcgcgt tgtcaacaat
    10021 ctggagggca gaaacatctc tgcccttttt cttgcctgcc atattgtgtg ttacacatca
    10081 caactttctc ttttgttgct gcataagtag acaaactttg tgaccgcctt catatctggc
    10141 cgatttcgtt ttttctgatc gatttacatt acgttaaata tgcttattat gaacatgtgt
    10201 ttaaatctga acacgtgctc taatagtctt attgactgta atgactattt tacccaattt
    10261 cttcgttggc aatttattgc cagggggcgt gctatgcgcg aaaagaacta catagtaatt
    10321 attggttcgg cgaatattga tgtggctggg tattcacatc atccattgaa ttatgcggat
    10381 tcgaatcccg gaaaaattaa atttacccca ggtggcgtgg gaaggaatat cgctcataat
    10441 ctggcgttac tggggaaaaa tgcctggctt ttaagtgcag taggtggtga tttttatggg
    10501 caatcattac tggcgcaaac taatcaatcc ggtgtttatg tcgataaatg cttaattgtt
    10561 ccgggtgaga atacatccag ctatttatca ctgctcgata atacaggaga aatgcttgtc
    10621 gcgataaatg atatgagtat tagtgactgt atttctgcag aatttcttgc ccagcatcag
    10681 gagtttattc gcggtgcaaa ggttattgtc gcggattgca accttagtga agaggcgctg
    10741 gtctgggttc tggaaaatag cggggaaacg cctgtattta ttgatccggt atcggcatgg
    10801 aaatgcgtca aaatccgcga tcatctgagc aaaatccata cgttaaaacc taaccgtctt
    10861 gaagccgaaa cgttgagcgg aattgcactt tccggacgtg aagatgtcgc aaaggtcgcc
    10921 gcctggtttc atcaacacgg cctgaaccgc ctggtgctga gcatgggagg ggatggcgtt
    10981 tactacagtg atatcaacgg tgaaagtggg tggtcggccc ctattaaaac gaatgtcatc
    11041 aatgttactg gggcaggcga cgccatgatg gcggggctgg cctcctgttg ggtagacggt
    11101 atgccgttta tcgattctgt tcgatttgcg cagggatgtt cgtcaatggc acttgcctgt
    11161 gaatatacca acaaccctga attatcaatt gccaatgtta catcgttagt ggagaacaca
    11221 gaatgtctga attaactctg tcacctgaat tattacaaat atccgctgaa gtacaggatg
    11281 ctttaaaaaa taaaaaaccg gttgttgcgc tggaatcgac cataatttct cacggaatgc
    11341 cattcccaca aaatgcccaa accgcaattg aagtagaaga aacaattcgt aaacagggcg
    11401 cagtaccagc cactatcgcc attattggcg gtgtgatgaa agtgggctta agtaaagaag
    11461 aaatcgaatt gctgggccgt gaaggacata acgtgacgaa agttagtcgt cgtgatttac
    11521 cttttgtcgt tgctgcaggg aaaaatggcg cgactaccgt tgcatcaacc atgattattg
    11581 cagcactggc tgggattaaa gtttttgcaa ccggaggtat tggtggtgtg catcgcggag
    11641 cggaacatac cttcgatatt tctgccgatt tacaggaact ggcaaatacc aatgttactg
    11701 ttgtttgtgc gggggcaaaa tctattctcg atttaggatt aacaacagaa tatttagaaa
    11761 ccttcggtgt accgttaatt ggttatcaga caaaagcgct gcctgcgttt ttctgccgaa
    11821 ccagcccatt tgaagtcagc attcgtcttg acagtgcccg cgaaattgct cgcgcgatgg
    11881 cagtgaagtg gcaaaccggt ttgaacggcg gcctggtggt agcgaacccg atcccggaac
    11941 agtttgcgat gccagaagaa accatcaatg cagcaattga tcaagccgtt gctgaagccg
    12001 aagaacaggg tgttattggg aaagagagta caccgttcct gctggcgcgt gttgcggagc
    12061 taaccggcgg tgatagcctg aaatccaata ttcaactggt ctttaataac gccattctgg
    12121 cgagcgaaat tgccaaagaa tatcagcgtc tcgtgggtta atttctggcg ggcagaaagc
    12181 cgtcgcccgt actcatcaaa cataccaaaa cctggcgctg acgccgggtt tactggcatg
    12241 aagggaaaat cattatggat ataatgagaa gtgttgtggg gatggtggta ttgctggcaa
    12301 ttgcctttgt gttgtcggta aataaaaaga gcatcagctt gcgcacggtt ggcgctgcgc
    12361 tgctgctgca aattgccatt ggtggcatca tgctctattt tcccccggga aaatgggcag
    12421 tagagcaggc ggcattgggc gtccataaag tgatgtccta cagtgacgca ggaagtgcct
    12481 ttatttttgg ttcgctggtc ggtcccaaaa tggatgttct gtttgacggt gcgggcttta
    12541 tcttcgcctt tcgcgtactc ccggcgatta ttttcgttac tgcgctcatc agtctgctgt
    12601 actacattgg cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagctc
    12661 ttaacatcag caaaatcgaa tcttttgtcg cagtcacaac tattttcctc gggcaaaatg
    12721 agatcccggc gatcgttaaa ccgtttatcg atcgcatgaa tcgcaacgag ttgtttaccg
    12781 caatttgtag cgggatggcg tccattgctg gttcgatgat gattggttat gccggaatgg
    12841 gcgtaccaat tgactacctg ttagcggcat cgctgatggc gatcccaggt ggtattttgt
    12901 ttgcacgtat tcttagcccg gccaccgagc cttcgcaggt cacatttgaa aatctgtcgt
    12961 tcagcgaaac gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc
    13021 tgaaaatcgc cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga
    13081 tcaacggcat tattggtgga gttggcggct ggtttggttt tgccaatgcc tctctggaaa
    13141 gtatttttgg ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg
    13201 ccaatcttgc gggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc
    13261 tgagtttctc cccatacctg caaacgggcg gcacgctgga agtgaaaacc attgcgatta
    13321 tctcctttgc gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat
    13381 tttcggctat ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg
    13441 cagcagcaac gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg
    13501 cgtaatgctc gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc
    13561 tattgcaaat aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag
    13621 cgccagtcct gacaactgct ttctgttttt aatgagatac cctttcttgc ttttagttaa
    13681 taaaccgtcg tgaatgaatt gcgcgagaac atataaaaga tgtcgataag aaacgcctaa
    13741 atactctgcc gcttgcgtat gcttttcgtg ataaagatca ccttcctgcg agagtaatat
    13801 aaaagctgcc aggcgattaa ctaacggaaa tgattgattc tgagttaaag aaacaatatt
    13861 acgataattt ttatgactta aggtgacgca gagttttcgt aaaaatagcg tgtcgtttaa
    13921 taacagcggg cggtaatgtt tcatagagag cgcaaggcac caacactctt caatagcctg
    13981 caccgcacgc ggttcatggt ctttgtcgat taactcaatc tcaccaataa aacagggggc
    14041 ggcaaagaaa tcgatcagcg acacgcgacc attagcaagc gtggcgtaaa gcctggcgcg
    14101 gcctcgcgtc agataaaaca gccaggacgg ctgttgccct tcctgcacaa tgtagtcacg
    14161 cgctaaaaaa tgaaacagcc gcgtatcggc tgaaacatcc gttaaaaagc aatccttaaa
    14221 cgtggattca ctcatcaatt gctgcttaag atcattattg tggatttctt tcacgatgtt
    14281 tatccgctat gagatttctc atatttagca tacgtacgcg gttggtaaga ttcaatcatc
    14341 ggcccggtcg aattttcttt gctgtagaaa aaataacgca actggaaaca gaggaaataa
    14401 acaatggaaa agagaaaaat tattctggat tgtgatccgg gtcatgatga tgctattgca
    14461 atgatgatgg cagcgaaaca tccggcaata gatttattag gcatcactat tgtagcgggt
    14521 aatcagacgc ttgataaaac attaattaat ggcctgaatg tttgccagaa actggagatt
    14581 aatgttccgg tttatgcggg gatgccgcaa cccattatgc gtcaacaaat cgttgccgat
    14641 aataatcacg gtgaaaccgg actggatggc ccggtattcg agccgctgac ccgccaggca
    14701 gaaagcactc atgcggtgaa atacatcatt gataccctga tggcaagcga tggcgatatc
    14761 actctggtgc cggttggtcc gctttcaaat atcgcggtgg caatgcgtat gcaacccgcg
    14821 atcctgccca aaatccgtga aattgtgcta atgggcggcg cttacggtac aggcaacttc
    14881 acgccatctg ccgagttcaa catctttgct gacccggaag ccgcacgcgt ggtgttcacc
    14941 tccggcgttc cgttagtgat gatgggcctc gatcttacca accagaccgt ttgcaccccg
    15001 gacgtaattg ctcggatgga aagggcaggc ggccctgccg gagagctgtt cagcgacatc
    15061 atgaacttca ctctcaaaac gcagttcgaa aactacggcc tggctggcgg cccggtgcac
    15121 gacgccacct gcatcggtta tctgattaac cctgatggca ttaaaaccca ggagatgtac
    15181 gtcgaagtgg acgtcaacag tggcccttgt tatgggcgta ccgtctgcga cgagctgggc
    15241 gttcttggca agcccgctaa taccaaagtc ggcatcacta ttgataccga ctggttctgg
    15301 ggcttagtcg aagagtgcgt gcgcggctac atcaaaaccc attaagtgtt gataacaagc
    15361 cgggccggta tcgcaagatg caggcctggc tatttctgat gggcagaaag ccgtcgccca
    15421 atatgacatg aagagataat cactatggat gtcatgagaa gtgttctggg aatggtggta
    15481 ttgctgacga ttgcgttttt actgtcagta aacaagaaga agatcagcct gcgtaccgtt
    15541 ggcgcggcgt tagtgttaca ggtcgtgatt ggcggcatta tgctttggtt accgccaggg
    15601 cgttgggtcg cagaaaaagt cgcttttggc gtccataaag tgatggcgta cagcgacgcg
    15661 ggtagcgcat ttatcttcgg ttctctggtc ggaccgaaaa tggatacctt atttgatggc
    15721 gcaggattta tctttggttt cagggtgtta ccggcaatta tcttcgtcac cgcgctggtg
    15781 agtattctct actacatcgg tgtgatgggg atcttaattc gaattctcgg cggtatcttc
    15841 cagaaagcat taaatatcag caagatcgag tcattcgtcg cggtcactac cattttcctc
    15901 gggcaaaacg aaattccggc aatcgtcaaa ccctttatcg atcggctgaa tcgcaatgaa
    15961 ttatttacag cgatttgcag cggtatggcc tcgattgctg gttcgacaat gattggttac
    16021 gccgcactgg gcgtgcctgt ggaatatctg ctggcggcat cgttaatggc gatccccggc
    16081 gggatcttgt ttgcccgcct gttaagcccg gctacggaat cttcgcaggt ttcctttaat
    16141 aacctctctt tcaccgaaac accgccaaag agcattattg aagccgctgc aacaggggca
    16201 atgaccgggc tgaaaatcgc cgcaggtgtg gcgacagtgg tgatggcatt tgttgcaata
    16261 attgcgttga ttaacggtat tatcggcggt gttggcggct ggtttggttt tgaacatgct
    16321 tcgctggagt cgattttagg ttacctgttg gccccattgg cgtgggtgat gggggttgac
    16381 tggagtgatg caaatcttgc cgggagtttg attgggcaga agctggcgat caatgaattt
    16441 gtcgcttatc tcaatttctc gccatatctg caaacgggtg gcactctgga tgctaaaacc
    16501 gtggcgatta tttctttcgc gttgtgcggt ttcgctaact ttggttctat cggggtggtg
    16561 gtgggggcgt tttctgcggt tgcgccacac cgtgcgccgg aaatcgccca acttggttta
    16621 cgcgcgctgg cggcggcgac actttctaac ctgatgagtg ctactattgc aggattcttt
    16681 attggtttag cgtagtgcgc aatttatgag ccggataaga tgcggtcaag cgtcgaatcc
    16741 ggcaacattg ttacaccatt ggcactaatg aaagcgcgtt atcggcagac agggtagggt
    16801 tgtttaagct gccgctggcg cgcgaaattg ccgcgcaggc taccgcaaaa cgggcgctgt
    16861 cgcggaaact gtatccttca agaaagctat ataccaggcc cgccataaaa ccatcgtcag
    16921 caccaaaact gtctactgtc gtatgcgctg gcgcggtcag caaaaattgt tctccatcct
    16981 tatcgctgca aaatacagat tcatctttca gataaacaaa aatgcgttta acaccttgtt
    17041 gatgcagcga atttacagct ctgatgcgat cgttgtcgtc tttaatcggt tgcccccaca
    17101 aaatttccag ttcattttgt gttggtttca gagtgtggat ccggctgtac cagtttttta
    17161 ctttgttcgc tttaaattcg gaaacggtat cgacaaacat cggaatttca tcagcaatgg
    17221 taaagaccca ctccagggct tcaggtgtga gattacaatc agccagcacc acaccagcat
    17281 ggcgaatcaa gtcccgtgaa gtattgagca gttgaggcgt tagctgttgc agaatatgtg
    17341 tgtcattgat tgccaggata gtttcttcct gcttattagc gatagcgaga taagttgcag
    17401 tgctgtgtcc atgcagacga atgcagtttg aaacgttcac gcccgcgcgg cgcgtttctt
    17461 ccagtaatgt ctcgccgtaa aaatcgttgc caatagctga tattaaatgc acatcccgac
    17521 caagcagagc caggttatgc gcgatattac gtccgacgcc gcctgcggaa cagtgaacag
    17581 aacctggatg agaggctgct tgcgggtaac ggatatccgc cattccgcga atatccatat
    17641 tgattgcacc cacaaccaca cagtaatcct gctcggtaag gatatatcct ttgcctttaa
    17701 tcaaaccctt gcgcatcaaa tccataatat gagcggcaac acgtgagcgg ctgatttgca
    17761 gaatatcggc gatttcattc tgttgaatca gcgggttacg ccgtaaaatt ttaagaatct
    17821 gcttttccct gtcgttcatc gatcaggcca ccgctttttc agtttgttgc gctttcagcc
    17881 aggcgatctc ttctgcccag atatccgggt tgatggtttc gaggatcagc ggaatgccgt
    17941 cgaaacggtc gtcctgcatg atccagcgga acgcatcatg accgatattg ccttcaccga
    18001 ggctatgatg gcggtcaacg cggctgccaa aggtgctttt cgcatcgtta aggtgcatcc
    18061 cgcgcagata cttaaagccg acagtacggg caaaatccgc gaatgttttc tcgcattcgg
    18121 ctggagtacg caaatcatac ccggcagcga aagcatggca ggtatcaatg cagacgccga
    18181 cgcgggattt atcttccacg ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta
    18241 agttactgcc ctgaccggcg gtgttttcta tcaccgctgt cacaccttga gttttatcca
    18301 gcgcaatgtt gatggattcg gcaatacgcg caaggcaatc ctcttctgaa atctgcatca
    18361 gatggctgcc agggtggaag ttgagcaaag aaagccccag ctgttcgcaa cgctgcattt
    18421 catctataaa ggcatcgcgc gatttttcca gagcttcagt gaccggatgt ccgaggttaa
    18481 tcagataact gtcgtgggga agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg
    18541 ctttgaattc atcgatggtt tgcgtcgtga gcggtgcggc acgccactga cgttggtttt
    18601 tggtgaacaa ggcaaacgcg gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca
    18661 gaccgccagc agcactaacg tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc
    18721 cgctggagga aaacggtaat gatagcgggt taacaggagc aagatgtagt ggtttatgcg
    18781 atgacgcttt gaatcacata gttaatcgca ccaccaccaa caatcagcca ggcaaacagt
    18841 accagtgcca tcagcagagg tttcgcccca gcttttttca gcgcgctgac gtgagtggtc
    18901 agacccagcg ccgccatcgc cattgccagc aggaaggtat ccagcgttac cagcatgttc
    18961 accacgctct gcggtaacag gtggaacgag ttaaagatgg caactacgat gaacaagatg
    19021 gcaaaccacg gaatagtgat tttgcttttc tcgccgctgt tcgccccaga cagctgttta
    19081 acacgcgccg ccagcaggat gaggaacgga gccagcatca tcacgcgcag cattttggaa
    19141 ataactgctg cgttttccgc atccgggctg atggcatgac ctgccgccac cacctgcgcc
    19201 acttcgtgca cagtagaacc aatgtagata ccgaaagttt ccggactaaa ccattgagac
    19261 atcagcggat atatcgccgg gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt
    19321 gcaacagcca cggttacttt actggcttcc gctttcacta ccggctcagt cgccagtacc
    19381 gcggcagcac cacagatact gctaccggca ccgatcaacc agctggtgtg cttatccaga
    19441 ccaaacactt tctgccccag gaagcaagcc agcaggaagg tactggacag cgtcaacacg
    19501 tcaatgatga tcccactgat accgacatcg gcaatttgcg agaacgtcag acggaagcca
    19561 taaagaatga tacccagacg taataaatat tgcttggcaa acagcacacc accgtcacag
    19621 cttttccaga tgtgcggata gatggtgttg cctaaaacca tccccaacaa gattgcgagg
    19681 gtgagggcac taaacccggc acccgcaacc gcgggaatgg aaccacccca cagggcgacc
    19741 ccggtgataa ctgcactcag ggctaacccc ggaataaaat gccacagtgt acgatgttgt
    19801 ttctgtaagg tgatattcgt cataaccctc tcctttaacc gggcataagg ttacgactaa
    19861 ctggtttaaa aataaaattg attatatatt tataattaat ctttataagt ggtaagcgac
    19921 tatgcacatc accctccggc agttggaagt ttttgcagaa gtattgaaaa gtggatccac
    19981 cacccaggcg tcggtgatgc tggcgttgtc gcaatcagca gtgagcgcag ccttgaccga
    20041 cctggaaggg cagcttggcg tgcaactgtt tgatcgcgtg gggaaaagac tggttgttaa
    20101 tgaacacggg cggctgctct atccgcgtgc gttggcattg cttgaacagg cggttgaaat
    20161 cgaacaactg tttcgcgaag acaacggcgc gattcgtatc tatgccagta gtaccatcgg
    20221 taactacatt ctgcctgcag ttatcgcccg ttatcgccat gattatccgc agttgccgat
    20281 tgaacttagc gttgggaaca gccaggacgt gatgcaagcg gtgctggatt tccgcgttga
    20341 tattggcttt attgaaggac cgtgccacag cactgaaatc atttctgaac cgtggctgga
    20401 agacgagctg gtggttttcg ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt
    20461 agaacagctg gccgctgcgc cgtggatcct gcgtgaacgc ggttccggca cgcgggagat
    20521 tgtcgattat ctattgctgt cacatttacc gaagtttgag atggcgatgg aactaggtaa
    20581 ctccgaggca atcaaacatg cggtgcgtca tgggttggga attagttgcc tgtcgcgacg
    20641 ggtgattgaa gatcaattgc aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg
    20701 cctgatgcgt acgttgtggc gtatacatca tcggcaaaaa cacctttcca acgcgctacg
    20761 gcgctttctg gactattgcg atcccgcgaa tgtaccgcgt taagttgctg tacaagaaca
    20821 tgctggtgct gtgtcgattt cgtgacgcag cgccctcagc atgcattcgc cagaaaagag
    20881 attggctgct ttacttataa tccctgggcg atcatgaagg tgtcttataa ccgtgtattt
    20941 ctgccggaag gattgccaat cgtctgctac aatcgcgcct catttttaag atggatagca
    21001 tttttgtatg gtttccgaaa ctaaaaccac agaagcgccg ggcttacgcc gtgaattaaa
    21061 ggcgcgtcac ctgacgatga ttgccattgg cggttccatc ggtacaggtc tttttgttgc
    21121 ctctggcgca acgatttctc aggcaggtcc gggcggggca ttgctctcgt atatgctgat
    21181 tggcctgatg gtttacttcc tgatgaccag tctcggtgaa ctggctgcat atatgccggt
    21241 ttccggttcg tttgccactt acggtcagaa ctatgttgaa gaaggctttg gcttcgcgct
    21301 gggctggaac tactggtaca actgggcggt gactatcgcc gttgacctgg ttgcagctca
    21361 gctggtcatg agctggtggt tcccggatac accgggctgg atctggagtg cgttgttcct
    21421 cggcgttatc ttcctgctga actacatctc agttcgtggc tttggtgaag cggaatactg
    21481 gttctcactg atcaaagtca cgacagttat tgtctttatc atcgttggcg tgctgatgat
    21541 tatcggtatc ttcaaaggcg cgcagcctgc gggctggagc aactggacaa tcggcgaagc
    21601 gccgtttgct ggtggttttg cggcgatgat cggcgtagct atgattgtcg gcttctcttt
    21661 ccagggaacc gagctgatcg gtattgctgc aggcgagtcc gaagatccgg cgaaaaacat
    21721 tccacgcgcg gtacgtcagg tgttctggcg aatcctgttg ttctatgtgt tcgcgatcct
    21781 gattatcagc ctgattattc cgtacaccga tccgagcctg ctgcgtaacg atgttaaaga
    21841 catcagcgtt agtccgttca ccctggtgtt ccagcacgcg ggtctgctct ctgcggcggc
    21901 ggtgatgaac gcagttattc tgacggcggt gctgtcagcg ggtaactccg gtatgtatgc
    21961 gtctactcgt atgctgtaca ccctggcgtg tgacggtaaa gcgccgcgca ttttcgctaa
    22021 actgtcgcgt ggtggcgtgc cgcgtaatgc gctgtatgcg acgacggtga ttgccggtct
    22081 gtgcttcctg acctccatgt ttggcaacca gacggtatac ctgtggctgc tgaacacctc
    22141 cgggatgacg ggttttatcg cctggctggg gattgccatt agccactatc gcttccgtcg
    22201 cggttacgta ttgcagggac acgacattaa cgatctgccg taccgttcag gtttcttccc
    22261 actggggccg atcttcgcat tcattctgtg tctgattatc actttgggcc agaactacga
    22321 agcgttcctg aaagatacta ttgactgggg cggcgtagcg gcaacgtata ttggtatccc
    22381 gctgttcctg attatttggt tcggctacaa gctgattaaa ggaactcact tcgtacgcta
    22441 cagcgaaatg aagttcccgc agaacgataa gaaataagtt tcctcccttc cttgctaagc
    22501 cctctcaacc gagagggctt tttcaattcc atttccctga caaatcatgc ggatataaaa
    22561 tttaacattt ggattgataa ttgttatcgt ttgcattatc gttacgccgc aatcaaaaaa
    22621 ggctgacaaa tcagaggctg ttccggcttt ctgggatgat cacctgcata aaaaataagt
    22681 ccaccgcgat gctgccgtac gcaaggggac gtgaagaaga tgtgagcgat aacccatttt
    22741 attttcgtag ttacctcatg gagatatgga atgtttaggt tgaacccttt cgtacgggtc
    22801 gggctgtgtt tgtccgctat ttcttgtgca tggcctgtgt tagcggtcga tgatgatggc
    22861 gaaacgatgg ttgtcactgc atcttccgtt gaacaaaacc tcaaagatgc acccgccagt
    22921 atcagcgtca ttacccagga agacctgcag cgaaaaccgg tacagaatct gaaggatgtc
    22981 ctcaaagaag tgcctggcgt acaactgacg aacgaagggg ataaccgtaa gggcgttagt
    23041 attcgtggtc tggacagcag ctacaccctg attctcgtcg acggtaaacg cgttaactcc
    23101 cgcaatgccg tcttccgcca caatgatttc gatctgaact ggatcccggt cgattccatc
    23161 gaacgtattg aagtggttcg tggcccgatg tcgtcgctat acggctccga tgcgctcggc
    23221 ggtgtagtga atatcatcac caaaaaaatc ggtcagaaat ggtcgggtac cgttaccgtc
    23281 gataccacca ttcaggaaca tcgcgatcgc ggtgatacct ataacggtca gttctttacc
    23341 agtggaccat taattgatgg tgtgctggga atgaaagctt acggcagcct ggcaaaacgt
    23401 gaaaaggatg acccgcaaaa ctcaacgacc accgataccg gagaaacgcc gcgtattgaa
    23461 ggattctcca gccgcgacgg caatgtcgaa tttgcctgga caccgaatca aaatcacgat
    23521 tttactgccg gatacggttt cgaccgtcag gatcgtgatt ccgactcgct ggacaaaaac
    23581 cgcctggaac gccagaacta ctccgtcagc cataatgggc gttgggatta cggcaccagc
    23641 gaactgaaat actacggtga gaaagtcgag aacaaaaacc ctggcaacag cagcccgata
    23701 acttccgaaa gcaatacggt cgacggcaaa tacacgttgc cgctgacggc gattaatcag
    23761 tttctcacgg ttggcggtga atggcgtcac tacaaactta gcgatgcggt gaacctgacc
    23821 gggggaacca gctccaaaac gtctgccagc cagtacgcgc tgtttgtgga agatgaatgg
    23881 cggatcttcg agccgctggc gctgacgacc ggcgtgcgta tggacgatca cgaaacctac
    23941 ggtgaacact ggagtccgcg tgcctacctg gtttataacg ccaccgacac cgtaacggtg
    24001 aaagggggct gggcgacggc atttaaagcc ccttctctgt tgcaacttag ccctgactgg
    24061 acgagcaatt cctgccgtgg cgcatgtaag attgtgggta gcccggatct gaaaccagaa
    24121 accagcgaaa gttgggagct ggggctttac tacatgggtg aagaaggctg gctggaaggg
    24181 gttgaatcca gcgttaccgt tttccgtaac gatgtgaaag atcgtatcag catcagccgt
    24241 acgtctgacg tcaacgctgc accgggctac caaaactttg ttggttttga gacgggcgct
    24301 aacggacggc gcataccggt atttagctac tacaacgtta acaaagctcg tattcagggc
    24361 gtggaaaccg aactgaaaat tccgttcaac gatgaatgga aactgtcgat caactacacc
    24421 tacaacgatg gtcgtgatgt cagcaacggc gaaaacaaac cgctatccga tctgccgttc
    24481 catactgcta acggtacgct ggactggaaa ccgctggcgc tggaagactg gtcattctat
    24541 gtttctggtc actataccgg gcagaaacgc gccgacagcg cgacggctaa aacaccgggc
    24601 ggttatacca tctggaatac cggcgcggcc tggcaggtga ctaaagacgt caaactgcgc
    24661 gcaggcgtgc tgaaccttgg cgacaaggat ctcagtcgtg acgactacag ctataacgaa
    24721 gacggacgtc gttactttat ggcagtggat tatcgcttct gatgagaaga tgcccggcga
    24781 accgggcgga ctttcacttc agtaaatact gcgcatggaa gcgcaggtga tcctctataa
    24841 aagaggcgat gaagtagtaa ctgtgatcat atcccggctg aatacgcagc gtcatcggcc
    24901 acgctttctg gcgcgcggct tctgccagta ccgcaggttg caactggtcg gcaagaaact
    24961 gatcattatc gccctgatcg ataagcgtcg ggatcgcatc ctgcgcgtta ctggcataca
    25021 tcagcgcgca actgtcccat tccagccatg catttttgtc ctcacctaaa tagctgctaa
    25081 acgctttgat tccccacggg acgctgcacg gattcacaat tggcgcaaag gccgaaacgc
    25141 tggtgtattt acccggattt ttcagcgcca taatcagcgc accgtgacca cccattgagt
    25201 gaccgctaat ggcgcagcgg tcgctgacat taaattgcga ctgaaccagc gccggtaatt
    25261 catcgcgcag ataatcatac atccggtaat gcgtcgccca cggcggttgc gtggcattaa
    25321 gataaaagcc tgcgccctgg cctaaatcgt agccatcgtc gttggcaacc ttttcgccgc
    25381 gcgggctggt gtctggcatc accagtacaa tccccagttc tgccgccacg cgctgggcac
    25441 ccgccttggt ggtgaagttc tcgtcattgc aggttaatcc ggaaagccag tacagcactg
    25501 gtggcggggt gtgatcacga gggggtggga gaaagatact gaacgtcatc gggcagttta
    25561 aggtactgga gtcgtgtcgc cagcgttgct gccagccttc aaaacagcgg tgctcttcga
    25621 gcatttccat gcgtggctcc ttgttgtgtt gtttgcaatt tcttcatcat accgtttatt
    25681 caatgtgctg tgagtaactt tcacttccgt atttgcataa cgatgtttta acatctgctg
    25741 atgaaaggca gcggcaatta caataattat cgctgtgaat actggattat gtgcgccgcc
    25801 tcacgcacaa taatcaggct gtaaatcagc ttaataactt tgcccccacg cagggcggag
    25861 gcgtcacacc tgcaggagaa atcataaatg ccatcactca gtaaagaagc ggccctggtt
    25921 catgaagcgt tagttgcgcg aggactggaa acaccgctgc gcccgcccgt gcatgaaatg
    25981 gataacgaaa cgcgcaaaag ccttattgct ggtcatatga ccgaaatcat gcagctgctg
    26041 aatctcgacc tggctgatga cagtttgatg gaaacgccgc atcgcatcgc taaaatgtat
    26101 gtcgatgaaa ttttctccgg tctggattac gccaacttcc cgaaaatcac cctcattgaa
    26161 aacaaaatga aggtcgatga aatggtcacc gtgcgcgata tcactctgac cagtacctgt
    26221 gaacaccatt ttgttaccat cgatggcaaa gcgacggtgg cttatatccc gaaagattcg
    26281 gtgatcggtc tgtcaaaaat taaccgtatc gtgcagttct ttgcccagcg tccgcaggtg
    26341 caggaacgtc tgacgcagca aattcttatt gcgctacaaa cgctgctggg caccaataac
    26401 gtggctgtct cgatcgacgc ggtgcattac tgcgtgaagg cgcgtggcat ccgcgatgca
    26461 accagtgcca caacaacgac ctctcttggt ggattgttca aatccagtca gaatacgcgc
    26521 cacgagtttc tgcgcgctgt gcgtcatcac aactgattaa aaggcaggaa ccatggagcg
    26581 caacgtcacg ctcgattttg ttcgcggcgt cgccattctg gggatcctgc tattaaacat
    26641 cagcgccttt gggctaccaa aggcggctta tctcaatccc gcctggtacg gcgctattac
    26701 gccgcaggat gcatggacct gggcatttct cgatctcatc ggccaggtga aattcctcac
    26761 gctttttgcg ctgctgtttg gtgcgggcct gcaaatgttg ctgccccgtg gcagacgctg
    26821 gatccagtcg cggttaacgc tgttagtctt gctgggtttt attcacggtt tattgttctg
    26881 ggacggcgat attctgctgg cttacgggct ggtgggctta atctgctggc ggctggtgcg
    26941 cgatgcgcca tcggtaaaaa gcctgtttaa tacaggcgtc atgctttatc tggtggggct
    27001 tggcgttttg ctgttattgg ggctgatttc cgatagccag accagccgcg cctggacgcc
    27061 ggatgcatcg gctattttgt atgaaaaata ctggaagctt cacggcggcg ttgatgcgat
    27121 cagtaatcgt gccgatggtg ttggcaacag tttactggca ctgggcgcac agtatggctg
    27181 gcaactggct gggatgatgc tcattggtgc cgcattgatg cgcagtggct ggctgaaagg
    27241 gcagttcagc ttacgtcact atcgtcgtac tggttttgtg ctggtggcga ttggggtgac
    27301 cattaacctt cctgccatcg ccctgcaatg gcagctggac tgggcgtatc gctggtgcgc
    27361 cttcttactt cagatgccgc gggaactgag tgcgccgttt caggcgattg gctatgcgtc
    27421 gctgttttat ggcttctggc cgcaattgag ccgctttaag ctggtgcttg cgatcgcctg
    27481 cgtcggacgg atggcgctga ccaactatct attgcaaacg ctgatttgta ccacgctttt
    27541 ttaccacctc ggtttgttta tgcattttga ccgcctggag ctgctggcgt ttgttattcc
    27601 ggtatggctg gcgaatatcc tcttctctgt tatctggctg cgttacttcc gccaggggcc
    27661 ggtggaatgg ctctggcgtc agttaacttt gcgtgctgcc ggaccggcaa tatctaaaac
    27721 atcaagataa cgatctggat cacattcgtt aacaaaacgg ctgtaaccgt ttccattgct
    27781 gtgactcgat tcacgaagtc ctgtattcag tgctgacaaa atagccgcca gcaagcagtc
    27841 atttactgca atctcataac aggtagtgaa tatgatcacc attcgtgatg tagcgcgtca
    27901 ggctggcgtc tctgtggcaa cggtttcccg ggtgctcaat aacagcacgc tggtcagtgc
    27961 cgacacgcgt gaagcagtaa tgaaagccgt gagtgagctg gattatcggc caaacgccaa
    28021 tgcccaggcg ctggcaactc aggttagcga caccattggc gtggtggtga tggacgtttc
    28081 tgatgcgttt ttcggcgcgc tggtaaaagc ggtggatctg gtcgcccagc agcatcagaa
    28141 atacgtgcta atcggcaata gctatcatga agcggaaaaa gagcgtcacg ccattgaggt
    28201 gttaattcgc cagcgttgta atgcgttgat tgttcactca aaagcattga gtgacgatga
    28261 actggcgcaa tttatggata acattcccgg tatggtgtta atcaaccgcg ttgtgccggg
    28321 gtacgcccat cgttgcgttt gcctggataa tctcagcggt gcccgaatgg cgacgcgcat
    28381 gttgctgaat aacggtcatc aacgtattgg ttatctttct tccagccacg gcattgaaga
    28441 tgacgccatg cgtaaagcag gctggatgag tgcgttgaaa gagcaggata ttattccgcc
    28501 ggaaagctgg attggcactg gtacgccgga catgccgggc ggtgaggcgg cgatggttga
    28561 actgctgggg cgcaatctac aacttaccgc tgtatttgct tataacgaca atatggctgc
    28621 tggcgcactg acagcattaa aagataatgg cattgcgatt ccgttacatc tctcaatcat
    28681 cggtttcgat gatattccca tcgcccgtta caccgacccg caattaacga ccgtgcgtta
    28741 tcccattgct tcaatggcga aattagccac cgaactggcc ttgcaggggg cagcaggcaa
    28801 tattgatcct cgtgccagcc actgttttat gccgacgtta gtgcgtcgcc attctgtcgc
    28861 aacacgccag aatgcggcga cgatcactaa ctcaacaaat caggcgatgt aaccgctttc
    28921 aatctgtgag tgatttcaca gtatcttaac aatgtgatag ctatgattgc accgttttaa
    28981 cgttgtaacc cgtatgtaac agtgaataat cactttcacc gaggtaatag cgtcataaca
    29041 acaattaaag ccgttttctg gagcgttacc gggcatggaa gaacgaattt taaaaagtga
    29101 gcttcggcgt tcagtaacac ttcattaact ctactgcccc gccgagcatt tatctcaagc
    29161 actaccctgc ataagaaaaa ccggagatac catgaataag aaggtgttaa ccctgtctgc
    29221 tgtgatggcc agcatgttat tcggtgccgc tgcacacgct gctgatactc gcattggtgt
    29281 aacaatctat aagtacgacg ataactttat gtctgtagtg cgcaaggcta ttgagcaaga
    29341 tgcgaaagcc gcgccagatg ttcagctgct gatgaatgat tctcagaatg accagtccaa
    29401 gcagaacgat cagatcgacg tattgctggc gaaaggggtg aaggcactgg caatcaacct
    29461 ggttgacccg gcagctgcgg gtacggtgat tgagaaagcg cgtgggcaaa acgtgccggt
    29521 ggttttcttc aacaaagaac cgtctcgtaa ggcgctggat agctacgaca aagcctacta
    29581 cgttggcact gactccaaag agtccggcat tattcagggc gatttgattg ctaaacactg
    29641 ggcggcgaat cagggttggg atctgaacaa agacggtcag attcagttcg tactgctgaa
    29701 aggtgaaccg ggccatccgg atgcagaagc acgtaccact tacgtgatta aagaattgaa
    29761 cgataaaggc atcaaaactg aacagttaca gttagatacc gcaatgtggg acaccgctca
    29821 ggcgaaagat aagatggacg cctggctgtc tggcccgaac gccaacaaaa tcgaagtggt
    29881 tatcgccaac aacgatgcga tggcaatggg cgcggtagaa gcactgaaag cacacaacaa
    29941 gtccagcatt ccggtgtttg gcgtcgatgc gctgccagaa gcgctggcgc tggtgaaatc
    30001 cggtgcactg gcgggcacag tactgaacga tgctaacaac caggcgaaag cgacctttga
    30061 tctggcgaaa aacctggccg atggcaaagg tgcggctgat ggcaccaact ggaaaatcga
    30121 caacaaagtg gtccgcgtac cttatgttgg cgtagataaa gacaacctgg ctgaattcag
    30181 caagaaataa gactgattgg gcgcagtcgt ttactgcgcc cttattgtat ggccaacaag
    30241 gtaaaattat atggtcagct caacgactcc gtcttccggg gaatacttgt tggaaatgag
    30301 cggtatcaac aagtcctttc ctggtgttaa ggcacttgat aacgttaatt taaaagtccg
    30361 gccacattct atccatgcat taatggggga aaacggtgca ggaaaatcga cattattaaa
    30421 atgcctgttt ggtatttatc aaaaagactc cggcaccatt ttattccagg gtaaagagat
    30481 cgatttccat tccgccaaag aggcactgga aaatggtatt tcgatggtac accaggagtt
    30541 aaacctggta ttacaacgtt cggtgatgga taacatgtgg ctggggcgat atcccaccaa
    30601 aggcatgttt gtcgatcagg acaaaatgta ccgcgaaacc aaagcgattt ttgatgaact
    30661 ggatattgat atcgatccgc gtgcgcgcgt cggcacatta tccgtttcgc aaatgcagat
    30721 gatcgaaatc gccaaagcgt tttcctataa cgcgaaaatt gtgattatgg atgaaccgac
    30781 ttcttcgtta accgaaaaag aggtcaatca tctgttcact attattcgta aattaaaaga
    30841 gcgcggctgc ggtattgttt atatctcgca taaaatggaa gaaatcttcc agttatgtga
    30901 tgaagttacc gtattgcgcg acggtcagtg gatcgccacc gaaccgctgg caggactgac
    30961 gatggacaag atcatcgcca tgatggttgg gcgttctctt aatcagcgtt ttcctgacaa
    31021 agaaaacaag ccgggcgaag tcatcctcga ggtgcgtaac ctaacgtcac tgcgccagcc
    31081 gtcgattcgc gatgtctcgt ttgatctgca taaaggggag atcctcggta ttgccgggct
    31141 ggtgggggcg aaacgtaccg atattgttga gacgttattt ggtattcgcg agaaatcggc
    31201 tggcaccatt acgttgcacg gcaaacagat caataaccat aatgccaacg aagccataaa
    31261 ccacggattt gcactggtaa ctgaggagcg ccgctcaacg ggaatttatg cctatctgga
    31321 tattggtttt aactcgttaa tttccaatat tcgcaactac aaaaataaag ttggtttact
    31381 ggataactcg cggatgaaaa gcgataccca gtgggtgatt gattcgatgc gggtaaaaac
    31441 gccgggtcat cggacgcaaa ttggttcgct ctccggtggt aatcagcaaa aggtgattat
    31501 tggtcgctgg ctactaacgc aaccagaaat attaatgctc gatgaaccga cgcgcggtat
    31561 tgatgtcggg gcgaagtttg aaatttatca gttaattgcc gaactggcga agaaaggcaa
    31621 ggggattatt attatctcct ctgaaatgcc tgagttgtta gggataacag accgtattct
    31681 ggtcatgagc aatggtctcg tttccggaat tgtcgataca aaaacaacaa cgcaaaacga
    31741 aattctgcgt cttgcgtctt tgcaccttta agattagggg cttcccatga gtgcgttaaa
    31801 taagaaaagt tttcttactt acctgaaaga gggcggtatt tacgtcgttc ttttagtttt
    31861 gctggcgatt attattttcc aggacccaac atttttaagt ctgttgaact taagtaatat
    31921 tctcacccag tcatcggtgc gtattattat cgcgctcggt gtggcagggt taattgtcac
    31981 ccaggggacc gatctttctg ctggtcgtca ggtagggctg gcggcagtgg tggctgcgac
    32041 attattgcag tccatggata acgccaacaa agtgttcccg gaaatggcga cgatgccgat
    32101 tgcgctggtt attctgattg tctgtgccat tggtgcggtg atcggtttga tcaacggtct
    32161 gattatcgct tatctcaacg tgacgccgtt cattaccacg ctcggcacga tgatcatcgt
    32221 ctatggcatc aactcgctct attacgactt tgtcggggcg tcgccaattt ctggttttga
    32281 cagtggcttc tctacctttg ctcagggctt tgtcgcgctg gggagtttcc gtctctctta
    32341 catcaccttc tacgcgttga ttgcggtggc gttcgtctgg gtgttgtgga acaaaacccg
    32401 cttcggtaag aacatttttg ccattggcgg taacccggaa gcggcaaaag tatctggtgt
    32461 caacgtcggc ctgaacctgc tgatgatcta cgcgttgtct ggcgtgttct atgcctttgg
    32521 cgggatgtta gaagccggac gtatcggctc tgccaccaac aacctcggct ttatgtatga
    32581 gctggatgct atcgcggcgt gcgtggtagg cggtgtatcg ttcagcggcg gtgtggggac
    32641 ggtgattggc gtggtgaccg gggtaattat ttttaccgtc atcaactatg gcctgacgta
    32701 tatcggcgta aacccatact ggcagtacat catcaaaggg gcgattatta tcttcgccgt
    32761 agcgctggat tcactgaaat acgcgcgtaa gaagtgatgt gattgtggat attgccggat
    32821 gcgctgcatc cggcaatcgg tgccaaatgc cggatgcgtc tctggtttta caacgttacc
    32881 gggtgttctt tctcgccttt cttaaacttc acttccccga gctcaataca acccaccggg
    32941 cagacgtgac cacaaagcag acaacccaca catttctcgg tattacaatg cggtgtgcgg
    33001 gttttctcgc tccattccat cgcctggtga ccgccgtcgt aacaggaaat ataacagcgt
    33061 ccacagccaa cacatttatc aagattgata tggggataga caatataact gcggtctaaa
    33121 tcttccgccg ggacaatatt gttattcgcc agacctacca tctcctgcaa cgaatcaaac
    33181 ccttgatcgg cgagataatg cgacaacccg ctcgccatat cttccactat ccgatagccg
    33241 tactgcataa tgccggtggt gacctgtaac gttgctgcgc cgagcaataa aaactccgcc
    33301 gcatcttccc aggtttcaat gccgccgata ccgctgatcg ggaaatcgcg cagttctgga
    33361 tgggtgcgca tttgctgaat aaagcgcagg gcgatcggtt ttaccgcttt accggaatat
    33421 ccggaaatac tcgatttacc gttcacgatc ggcataccga ttttctgatt aagatcgata
    33481 ttggtgatgg atttaacggt gttaattgcc gcaatgccat ctgcgccgcc gcgcttcgcc
    33541 gccagcgcca cttcgcacat atcaccgata ttcggtgtca tcttcgccag catcggcagc
    33601 gtggaacccc gtttaactgc ccgacaatat ttttctacca gctccgggct ttgcccgaca
    33661 tcgctaccca tcgcatgaga cgtcatttgc ggacaggaga agttacactc gatcatatcc
    33721 gcgccagctt cttgcaccag gcgcgccagc tcctcccatt gctgctcatt ttcccccatg
    33781 atcgaagcga tcaatacttt gtccgggtaa tcttccttca gccgacgcag ggcggccaga
    33841 ttctcttcca acggatgctc ggcaatctgc tccatatttt tgaagccgat aaaaccggta
    33901 tcttctttca ccagatgatc aaaacgcggc gagacttcgt tggcgataaa aaagccgatc
    33961 gttttaaaca ccacgccgcc ccaacctgtg tcgtaggctt tggcacacat ctcatagcag
    34021 ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa acttcacgcc gcaaaaggta
    34081 atcgaaagat ctttcgttaa catgagcaag ctccctctaa atagtgatga atcgcctcgg
    34141 cggcttcttt cccggttttc acggcataga ccacggtttt gtcaccctca acaatatcgc
    34201 cagcagcaaa gacttgcgga tcgcgggtct gataattttg tgttttgatg gtgttacgct
    34261 gcggctctaa cttcgcaaag gcatccagtc tggcatgctg accgacggcg agaataattt
    34321 tatctgccac catcgtcagt tcgcccggta accgtacatg cttaaacgtg actttattgc
    34381 cttccacggc tactggcgtg aatccatcga tgatcgaaac gcccagctcc cgggcgctgg
    34441 taaactcttt ttcgcttgcc ggaaactcat ctaactcttc acgcgctacg caagttaccg
    34501 cctgacagcc gagaactttc agcgtgctgg ctacgtccat cgcgacatca ccgccgccga
    34561 taattaatgc gctttgtgga atgctgatat cgccttgcgc ctgtcgtgca cgttgcaaga
    34621 agtcgacggc aatctcaacg tcgctatgct caaacagcgg tagcccggaa ccgcttgata
    34681 accccacggt gaccagtacc gcgcggtttt ccgctttcag ttgctccagg gtgagtgtgt
    34741 taccgacttc gttgttgcac ttaatggtca cgcccatttt ttcaatacgg gcgatctctg
    34801 catccagcac tgattgcggt aaccggaatt gcggaatacc gttacgcaac cagccaccgg
    34861 ggtgcgcttc tttctcataa atcgtgacgt cataaccctg gtttgtcagt gtcacactgg
    34921 cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt gccgagcgtt ttagtaccgg
    34981 gctgataaat ttccattccg gtttgttgtt caaaatcagt aacaaaacgc tgtaagcggc
    35041 cgatatcaat gggagcatca acaccggcac gggtacaacc gctttggcat aatttctccg
    35101 tcgggcacac tctggcacaa acggcaccga gggcattatt ttcgcgaatt gtctcggcag
    35161 cgcctttaaa attacgaaag tagattgagc gaataaattt ccccggatcg gtctgtgccg
    35221 ggcaagcctg actacaggga gcgtcgtgac ataataaaca gcgagaggct tctttaatcg
    35281 ccagtaaaga cgtaaaagcc ggagtgagtt catccagata attttgttgc ggcatgtgat
    35341 cttccttaat gctataggta attaaggcga gcgtttagta aataataatg tactgatatt
    35401 aaggctaagc gtgcggggga atttaaactg tgaaaagatt cacaaatagc tgcggttagg
    35461 aatgtgatat ccgcagctta tcaggtgaag taataattat tctcaataat taactggaga
    35521 ggggaatatt aacgatagaa aataagataa acaatcatgc cgatagataa aattccgaag
    35581 ccgatatatg ccaccatata agcgaaaaag agtccgggat tttttgctcg ccagacatac
    35641 accggttccg gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca gcctttccat
    35701 gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg caacgacaaa aaactgctgt
    35761 acatccataa ctactttcct tgtttctttt gtaattcaag taactgttcg ggtgtgacgg
    35821 cgggataccc ctgcgcatct tccggtacct ggtgcatagc cggaataggg accagcggcc
    35881 ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc cagcgcaccg aaacgggcgg
    35941 caaattcacg aatacgtact gacagcatat ctttcggtga cggtacggca taacactgag
    36001 cctgaatccc catatgcagc gcaataaata atgctcgctc acagtggaaa cgttgggtga
    36061 taataatgaa atcattagta tcgaaaactt tgcgtgtacg cacgatggaa tccagcgtac
    36121 gaaagcctgc gtaatcgaga acaatatctg atgggtcgac accagcagcg attaaatctt
    36181 tgcgcatggt catcggctca ttataacttt gcaatgcgtt atcgccgctc agtaatagat
    36241 aatttacctt accgctgtta taggcattaa tcgctccttg aatgcggtag cgataatact
    36301 gattaattac gccagtacga taatattttg ctgttccgag caccacaccg acctggcggt
    36361 aggggagatc ctgcaattcg tcgtagatat aaggcgcggt tttccagctc atccagcgat
    36421 cgaggccgag cacagtcaac agcagcaagc cgatcaggac taacaggctg aggaacacgc
    36481 gctttaacat gcaggagact cgatctaagg aaaatttcca tcaggctact ttacccgctg
    36541 gttttgcgca agaaacggca aactgtttgc cgggattttc gacaccagga gagggctggc
    36601 aagccggaca aggcgttcac gccgcatccg gcaccaggct taagcgagaa gtacgcggtc
    36661 gatactgtga cagccgagag ctttcagcgt tgcggaggtg gcatcccact gaatcaacgg
    36721 cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat aacccttgag
    36781 attcaataga attaacgctc cctgcaacgg tggcagagtc gggttgaatg cggcgttttc
    36841 agcgtagctg ccgctgaaaa ttcgaccgtc tttacattcc agcgcaacac cgcttggcga
    36901 cttactgtaa ggcatgtgcg aacggtttgc cgccgcaatc gccgcctgag aaagcgcatc
    36961 accagtcagc gcatagccgt gatcctgctc gtccatcagc agcgttttaa tctccagatc
    37021 tttcggccca aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc ggcccggcag
    37081 atgaatacgc agatccagac cgctgttcag ttcattcata aactgacggc agtgaccaca
    37141 aggcgtgtag ttaacggtga tggctgcaag cgctttttca ccactcaacc aggcgtggct
    37201 gatcgcgctt tgttcggcat gaacggtttg ctgcattgtc gcaccaataa attccatatt
    37261 ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt gcgccaacat taaaattcga
    37321 caatggcgta cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca gcgcgtcttc
    37381 gtccagcccc gttgcgctct tcagcgatga gacttgctcc ccggtcaaca aagcggggaa
    37441 gtacttgtct gccagaatag gttccagtgc agattgcaag ttatccgcaa gttgggcaaa
    37501 agcggtttga aaacgtggat gcatggcgtt gcctcataat catgtaatgg gataacagtt
    37561 tacgaacccg ttatggcttt atatgtgatc tgaatctcat taattatgca acgcgtgaaa
    37621 cttacatcaa aaatgcgcga cgcatcgcaa attttagccc attactgcca gaataatcgg
    37681 gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg ctagcgaact
    37741 aaatgcacct tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg cgtgcgaggc
    37801 agtccccatt gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat taagcaatgt
    37861 atggccaaat accgcgccga ggatgccgac gaaaatcacg caaactgcgc tgattgccgg
    37921 aataccgcca atactgccgc caaccgccat tgcaataggc gtggtgactg atttcggcag
    37981 gattgacgcg gcgatttccg gtgaagcgcc catcaacaat gccacggaag tccccgtcac
    38041 catcgcaacc acgctgccga taaaacagat ggtgatgatc gatttccagc gcgcgcggat
    38101 ctggtgtagc tgctcatata aaggataggc cagcgcgacg accgccggtt gcagcagatc
    38161 gtttagcact tcgctacctt taaagtagct gtcgtaagag atgccagtca gcattaaaaa
    38221 aggaatgatc accaccattg ctaccagcaa cgggttaagc aagggaaact tataccgtgc
    38281 cgccagtttg cgggcggcaa aaaagacaat caatgtcagc ggtaatgacc accagatatt
    38341 cgccatcatt cttctgatcc tttctgacct acgactttac gttcaccgtg caccagttgc
    38401 gaactccagc tcaccaccag aaaaaccacc agcgtactga ctgcacagga aaccactacc
    38461 gggccaaact gtgcgcggag caaatcaaaa tattgcatga cgcctacgcc aatcggcaca
    38521 aacaatagcg ccatatagcg aatcagtacg tagcaccccg gattgaccca ttttgccgga
    38581 agaatttgca aggccagcag gacaaacagg atcagcatac cgatgatgct gcccggaatg
    38641 gttaccggta gcagggaagc aatgaaaatg cctgcataca ggcaggcata aatcaggacg
    38701 aaagcgcgta aatattgcca gataatgttc agtgtcttac tcataatcaa caccccagat
    38761 gagatggatt catcatacaa ttaattcgta aaacgtgcta ccgatcacat taagaaatca
    38821 gcgcttattt tttcgctccc cggtattacg caatgagcag cgaactcagg catacatcaa
    38881 gcatagagag ggattgcagt ttgcagcaat ccctctttgc agacctttaa tccgctgacc
    38941 ataggcgcta acatagcgcc tcattttttt gcgggtgatg atatgcgtgt gttactggca
    39001 ccgatggagg gagtgcttga ctctctggtg cgtgaattgc tgaccgaagt taacgactac
    39061 gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt aaaagtcttt
    39121 catcgcattt gccctgagct acaaaacgcc agccggacac catctggtac gctggtgcgc
    39181 gtgcagttgt taggtcagtt cccacaatgg ctggcagaga acgccgcccg tgcggtggag
    39241 ttaggttcct ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt taacggtagc
    39301 ggcggcggcg cgacgttact caaagatcct gaactcatct accagggtgc aaaagcgatg
    39361 cgtgaagctg taccggcgca tttgcccgtc agcgtgaaag tgcgtctggg ctgggacagc
    39421 ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg ctggtgctac ggacctggtg
    39481 gtgcatggac ggacaaaaga gcagggttac cgcgcggagc atattgactg gcaggcgatt
    39541 ggcgagattc gccagcggct gaatattccg gtgattgcca acggcgaaat ctgggactgg
    39601 cagagcgcgc aacaatgcat tgcgatcagc ggctgcgacg cagtgatgat tggtcgcggg
    39661 gcgctcaata ttcccaacct gagccgggta gtaaaatata acgaaccgcg aatgccgtgg
    39721 ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg cgataccggg
    39781 ttatatcacg ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga atacgatgaa
    39841 gcaacgggat tatttcagca tgttcgggtg ttgaataatt cccctgatat tgcaagggct
    39901 attcaggcaa ttgatatcga taagctgcga taatagtgga atcgcagttt atcagagaca
    39961 atgaatcgct tattcagctt catcatcttg ttgcgctcct ttcatgagct aagccacata
    40021 ttgccactgg cgcaaggagc gcgcaggggg aggacaatcg cctcctcccc ctgcaccccc
    40081 gggctctggc gaacaaaatc gccgctgcgc ggtaccttca gcttatcccc ttcggctacc
    40141 gggtcgggcg cgaggtaacg tccttgtaag acgcgccctc agcccacatc catgtgggct
    40201 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaacaccgg agctgtttgc
    40261 aaatcaagta cacaaaatat ctagttcctg acctgacaac ttatatgcca tatctgattt
    40321 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    40381 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    40441 ggaaaaaacg aagccctttg ccgttatcca taaacgatta agataaatcg gaagatatca
    40501 ttcacttagt gtatattttt ctggatatgt cgttttattg ccactgatgt attgccacgc
    40561 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccttttct tacctgatta
    40621 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    40681 gctggccggg tgtgctccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    40741 tgtgcaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    40801 gtggctggag tatcacgata atcaactcac ttccttaatt aataacgcac tacagaacgc
    40861 accggatatg caggtggctg aacagcgcat tcagctggct gaagcccagg cgaaggcagt
    40921 tgccactcag gatggtccgc agatcgactt ttctgcggat atggagcggc aaaaaatgtc
    40981 ggcagaaggg ttaatggggc cgtttgctct gaacgatcct gccgcaggta cgaccggtcc
    41041 gtggtacacc aacggtactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    41101 gaatcgggcg gaggtaactg ctcgcctggg tacggttaaa gcacgagcgg cggaacgcga
    41161 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    41221 ggcggcgtta aatactgtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    41281 cgatcgccag ctatatcaga acgggattac ttcttcagtt gaaggtgtgg aaaccgatat
    41341 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    41401 ggcacggtta agcgcactta caaatcacca gacaaaggca ttgaagctta atccagtcgc
    41461 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    41521 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ctaagcacca ttgatgcggc
    41581 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    41641 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    41701 acccattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    41761 cttgtctatc gccagctaca acaaagcggt ggttgaagcg gtgaatgacg tggcgcgggc
    41821 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    41881 tgccttgcgt gtggtaggtc ttgcgcaggc gcgctttaac gcgggcatca ttgctggttc
    41941 ccgcgtcagc gaagccagaa tccccgcgct gcgtgagcgg gccaatggcc tgttattgca
    42001 agggcagtgg ctggatgctt ccattcaact cactggtgcg ttgggcgggg gttacaaacg
    42061 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    42121 cacaggttgc gattattacc gcctccgatt cggggatcgg caaagagtgc gcgttattac
    42181 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    42241 ataccgcgcg tgaggtagtt agccacggcg tacgtgcgga gatcgtgcag ctggatctcg
    42301 gtaaactgcc agaaggtgcg caggcgctgg agaaactcat tcaacggctg gggcgtattg
    42361 atgtgctggt gaataatgcg ggcgcaatga ccaaagcgcc gtttcttgat atggctttcg
    42421 atgagtggcg caagattttt accgttgatg tcgatggtgc atttttatgc tcacaaattg
    42481 cgacccgcca gatggtgaaa caagggcagg gcggtcgcat catcaacatt acgtcggtac
    42541 atgaacatac gccgctgccg gatgccagcg cttacacagc cgctaaacat gcgctcggcg
    42601 ggttaaccaa aacgatggcg ctggagctgg tcaggcataa gattttggtg aacgcagtcg
    42661 cgcctggggc gatcgccacg ccaatgaatg gcatggatga cagcgacgtg aagcccgacg
    42721 cggagccttc gattcctctg cggcgttttg gcgcaacgca tgagattgcc agcctggtag
    42781 cgtggctgtg ctcagagggc gcaaattaca ccaccgggca gtcgttgata gtggatggcg
    42841 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggttatt
    42901 tcaatgggta aaattttggt aggcatgata agacgcacaa gcgtcgcatc aggcatggtg
    42961 caccggatgt cggatgcggc gcgaacgcct tacccagggc aatggtttta cgcctgatga
    43021 tccggctttt tcttcccgcg ccgtttcagc caccagcgca cgcctaccac cagaaccacg
    43081 accagaatca accagaccca gtgcttcaaa tgctggtcga gagtgtgcaa ccacggcgca
    43141 atcacctgac caccagcgta accgatagtg gtaaaaatca acgcccaggc aaatgcgccg
    43201 agaatattaa gcggcagaaa gattttcggc ggcagctggc tggcaccaat cagcgtcgga
    43261 ccaatgaccc gaaagccata cataaaacgc gtaccaatga caaacagata cggatggcgc
    43321 tggataagtt tctgcgcccg ctcaatttta tcctgatgtt tcgagaaacg gcgtaacagc
    43381 ttgccgccaa accgccgccc gcacagatag agcacctggt cgccaatcat gccgccaagc
    43441 gccacagaaa gtaccaccag cgggaacttt aatagtccct gatgtgccgc aacgcctccc
    43501 agcaaagtca cggtttcacc ttccgccagg ctaccgataa ccagcgcggc ataaccatat
    43561 tgtgagatga gtgtattgag atccataggc cagtctgtct ccctttggtt gcctgtaagc
    43621 atacaccctg tgaatcattc acgctgaaaa aggacataca tcagacggtg caaattacat
    43681 ggatgaacaa aaaataatca caagctggct tatacttgaa ctgtcaggtt catgaactca
    43741 caacaagggg gcggtatgag ccatgtctgg ggactgtttt cccatcccga tcgtgaaatg
    43801 caggtgatta atcgcgaaaa cgaaacgatt tctcaccatt acacccacca cgttttgctg
    43861 atggcggcga ttccggtgat ttgcgccttc attggcacta cacagattgg ctggaatttt
    43921 ggcgatggca ctatcctgaa gttatcctgg tttaccggac tggcgttggc tgtcctgttt
    43981 tatggcgtta tgctcgctgg ggtcgcggtg atggggcggg tcatctggtg gatggcgcgc
    44041 aattacccgc agcgtccgtc acttgcgcac tgcatggtct tcgcgggcta cgttgcaact
    44101 ccgctgtttt taagcggtct ggtggcgctt tatccgctgg tctggttgtg tgcgctggtc
    44161 ggcacggtgg cgctgtttta caccggctac ctgctttacc ttggtattcc gagcttcctg
    44221 aatatcaaca aagaggaagg ccttagcttc tccagttcga cgctggcgat tggcgtgctg
    44281 gtactggaag tgctgctcgc cctaaccgtt atactatggg gctacggtta tcgtttgttc
    44341 tagaaagttc tttgacgttg cattgctggc gtaaatgtca gcaatgcagc atttcctcac
    44401 gattctcctt tgacgatcgc actttttgct cgttatgatg gcgtcgctag cctcagtaaa
    44461 tccttactcc ggcggtgcgc aacccgtgcg cgtgaaccac tatctgaatg ctcatcatgc
    44521 cgaaatttcg agtttcttta tttagcctgg ccctgatgct ggctgtgcct tttgcaccgc
    44581 aggccgttgc gaaaacggca gccgctacca ccgcttcaca accggaaatt gcctccggta
    44641 gcgcgatgat tgttgatctg aataccaaca aagtgatcta ttcgaaccac ccggatctgg
    44701 tgcgtccgat tgcgtctatc agcaaattaa tgaccgcgat ggttgtgctg gatgcacgac
    44761 tgccgctgga tgaaaaacta aaagtggata tcagccagac gccggagatg aaaggggtct
    44821 attcgcgcgt acgactgaat agcgaaatca gccgtaaaga tatgctgttg ctggcgctga
    44881 tgtcttcaga aaaccgcgcg gcggcaagcc ttgcacacca ttatcccggt ggttacaaag
    44941 cctttattaa ggcaatgaat gcgaaagcga aatcgctcgg aatgaacaac acgcgctttg
    45001 ttgaacctac cggattgtcg gtgcataacg tttcaactgc ccgtgactta accaaactgc
    45061 tcattgccag caaacaatat ccgttgatcg ggcagttaag tactacccgg gaagatatgg
    45121 caaccttctc taacccgacg tatacgctgc cgttccgcaa tactaaccat ctggtgtatc
    45181 gcgataactg gaatattcag ttaaccaaaa ccggttttac caatgcggcg ggccattgtc
    45241 tggtgatgcg tacggttatc aataataaac cggtggcgct ggtagtgatg gatgcgtttg
    45301 gcaaatatac ccattttgcc gatgccagcc gcctgcgtac ctggattgaa accggtaaag
    45361 tgatgcctgt tccagcagca gcgttgagct ataaaaaaca aaaagccgcc caaatggcgg
    45421 cggcggggca gacggcacag aacgattaag tgaatgacgg atggcgatac tatgccatcc
    45481 gtaattttta ctccacttcc tgccagtttt tccgcttact ggttttaccg atccccggat
    45541 tcatactgtt ggtcggatcg ttctcgcgat aaaacttctg caacgtctcc ggtgctttat
    45601 acaaatgacc gacgttatgc tcggcagggt attgcgcgcc acgctgctgt agcagttcca
    45661 gcatctgttc tttcaacgca tgcacatcca cgcctttttt cactatgtaa tcctgatgga
    45721 agacatagca cataaaatgg ccgtaataga gcttgtgcac cagctggctg tcgatctccg
    45781 gcggtaaatg ctcataccac tcggtgtcgt tacgccgcag agcgatatcc aacgccagaa
    45841 tgtcttcgac ttcatcggaa tgcaccgcct gataacgaat tgctgcgccc gcagcggcga
    45901 aacggtgtaa aaacgctttg ctgccttcct ccggcgtaca gacaaagaaa tcgccttcgg
    45961 cctgattgaa ataatctacc agccacgatt tggcttcacc gacgccatcg cccgccattt
    46021 ttaacagcag atgatgctcg tatttatcgc gccagttttt catgcgcggc ggtaaatggc
    46081 tggggaacag gtgaccgaat ttttgcatcg cacggtcagt aaaatgcgga cggaagaatt
    46141 tcactttctc cagcatcgca tcggtgcgtc ccttgagatt aaagaagaac ggcatcttgt
    46201 cggtgcctaa cttatcaatc atcaggaagg tgtctttgcc gtatttttcc gcaatatcgt
    46261 agatatcccg gtgcatatat tccccggcaa ccggcagatt ttcaaagtta gccagaatat
    46321 ggcggcggat ttcggtcagc acttccggct ggttggtgcc gatataaaac acctgctgat
    46381 ttttttccgc ttcgaaggta tctagacgta ccgcaaagac ggccagcttc ccggcgcaac
    46441 cagaagattc aaataaccga tccggatcgg cgttataacg tgcgggcgtg tcggcttcaa
    46501 tatcacgaac gcggtggaca tagtcataat cgtgggcgtg acgaccatcg tgacgcacat
    46561 cgtcatcttt gatgcgatca tcatccagct tgctaaggat ctgctccggc gtttcgccca
    46621 gatcaatccc cagatggttc accagcgtca gtttgccgtc ttcatttata cgcgcgaata
    46681 acgacatttc ggtatacgcc gggccacgtt gcaccagcga gccgccggag ttgttacaaa
    46741 taccgccgat aaccgatgcg cctatacacg acgatccaat caccgagtgc ggttcgcgtc
    46801 ccagcggttt gagggctttt tccagcgaat agagcgtggt gcccggatag gctagcacct
    46861 gttcgccctt gccaagaacg tgcagcttgt cgagacgcag ggtgctgatg ataacgacat
    46921 ctcgatcata gtcgttaccg tttggcgtcg atccttcggt caggcctgta ttggcagcct
    46981 gcatcagaat aattttgtcg gcggtgacgc aggctttcag cacccgccac aattccagta
    47041 gtgagccagg gaaaacgaca gccagcgcgt cgccctgacc agaacggaag cccttgcgat
    47101 agcgggccgt ttttgcggga tcggtgagca ggtgtgaatg accgaccaga cgggcaagtt
    47161 cattcaaaaa ggctttatta tcagttgttg tcatggaaga cattttccac tccttgtggt
    47221 ggcgaaaaaa tatcacaaac tagaatagcg ccttgtatga caattcagcg agtgaatatt
    47281 ttgaaaattc agacaataaa ctgagcattc tttttctctc tccatcatgc ttatggcaaa
    47341 ctacggtttt tgcaccattt tcgctgcctc gtcgggcaac agaggaagaa aaatccatat
    47401 gaaatggcta tgttcagtag gaatcgcggt gagtctggcc ctgcagccag cactggcgga
    47461 tgatttattc ggcaaccatc cattaacgcc cgaagcgcgg gatgcgttcg tcaacgaact
    47521 gcttaagaaa atgacagttg atgagaaaat tggtcagctg cgcttaatca gcgtcggccc
    47581 ggataacccg aaagaggcga tccgcgagat gatcaaagac ggtcaggttg gggcgatttt
    47641 caacaccgta acccgtcagg atatccgcgc catgcaggat caggtgatgg aattaagccg
    47701 cctgaaaatt cctcttttct ttgcttacga cgtgctgcac ggtcagcgca cggtgttccc
    47761 gattagcctc ggtctggcct cgtcttttaa cctcgatgca gtgaaaacgg tcggacgtgt
    47821 ctctgcttat gaagcggcag atgatggcct gaatatgacc tgggcaccga tggtcgatgt
    47881 ctcgcgcgat ccgcgctggg gacgtgcttc cgaaggtttt ggcgaagata cgtatctcac
    47941 ctcaacaatg ggtaaaacca tggtggaagc gatgcagggt aaaagcccgg cagatcgcta
    48001 ctcggtgatg accagcgtca aacactttgc cgcatacggc gcggtagaag gcggtaaaga
    48061 gtacaacacc gtcgatatga gtccgcagcg cctgtttaat gattatatgc cgccgtacaa
    48121 agcggggctg gacgcaggca gcggcgcggt gatggtggcg ctgaactcgc tgaacggcac
    48181 gccagccacc tccgattcct ggctgctgaa agatgttctg cgcgaccagt ggggctttaa
    48241 aggcatcacc gtttccgatc acggtgcaat caaagagctg attaaacatg gcacggcggc
    48301 agacccggaa gatgcggtgc gcgtggcgct gaaatccgga atcaacatga gtatgagcga
    48361 cgagtattac tcgaagtatc tgcctgggtt gatcaaatcc ggcaaagtga cgatggaaga
    48421 gctggacgat gctgcccgcc atgtactgaa cgttaaatat gatatgggat tgtttaacga
    48481 cccatacagc catttggggc cgaaagagtc tgacccggtg gataccaatg ctgaaagccg
    48541 cctgcaccgt aaagaagcgc gtaaagtggc gcgcgaaagc ctggtgttgc tgaaaaaccg
    48601 tctcgaaacg ttaccgctga aaaaatcggc caccattgcg gtggttgggc cactggcgga
    48661 cagtaaacgt gacgtgatgg gcagctggtc cgcagccggt gttgccgatc aatccgtgac
    48721 cgtactgacc gggattaaaa atgccgtcgg tgaaaacggt aaagtgctgt atgccaaagg
    48781 ggcgaacgtt accagtgaca aaggcattat cgatttcctg aatcagtatg aagaagcggt
    48841 caaagtcgat ccgcgctcgc cgcaagagat gattgatgaa gcggtgcaga cggcgaaaca
    48901 atctgatgtg gtggtggctg tagtcggtga agcacagggg atggcgcacg aggcctccag
    48961 ccgtaccgat attactattc cgcaaagcca acgtgacttg attgcggcgc tgaaagccac
    49021 cggtaaaccg ctggtgttgg tgcttatgaa cgggcgtccg ctggcgctgg tgaaagaaga
    49081 tcagcaggct gatgcgattc tggaaacctg gtttgcgggg actgaaggcg gtaatgcaat
    49141 tgccgatgta ttgtttggcg attacaaccc gtccggcaag ctgccaatgt ccttcccgcg
    49201 ttctgtcggg cagatcccgg tgtactacag ccatctgaat accgggcgtc cgtataatgc
    49261 cgacaagccg aacaaataca cttcgcgtta ttttgatgaa gctaacgggg cgctttatcc
    49321 gttcggctat ggtctgagct ataccacttt caccgtctct gatgtgaaac tttctgcgcc
    49381 gaccatgaag cgtgacggca aagtgacggc cagcgtgcag gtgacgaaca ccggtaagcg
    49441 cgaaggggcc acggtagtgc agatgtactt gcaggatgtg acggcttcta tgagccgccc
    49501 ggtgaagcag ctgaaaggct ttgagaaaat caccctgaag ccgggcgaaa ctcagaccgt
    49561 cagcttcccg attgatattg aggcgctgaa gttctggaat caacagatga aatatgacgc
    49621 cgagcctggc aagttcaatg tctttatcgg cactgattcc gcacgcgtta agaaaggcga
    49681 gtttgagttg ctgtaatgct ttagtaaggg cgcgacgttt gcgccctttg cctgatgttc
    49741 atatcttaac ttgctgaatt ttcgcccttt gtaggccgga taaggcgttt acgccgcatc
    49801 cggcaagaaa cgccgggcaa aatatgccct ttaagtattt atctgattct gcaactgaat
    49861 ccttccgctc aggctaactc tgtcattatc aactatgctt ttctcttaat tcgctgaaaa
    49921 caacagtaaa agaatgagga aagcatcatg ccactctcaa aggtctgggc aggttcactg
    49981 gttttgttgg cagccgtgag cctgccgctg cacgcggctt cccccgttaa agtcggttca
    50041 aaaatcgata ccgaaggcgc gctgctcggc aatatcattt tgcaagtact cgaaagccac
    50101 ggagtaccaa cggtcaataa agtgcaactc ggagcgactc ccgtggtgcg cggggcgatt
    50161 acttccggtg aactggatat ctatccggaa tataccggca atggcgcttt tttctttaaa
    50221 gatgaaaacg atgcggcatg gaaaaacgcg cagcaaggtt acgagaaagt caaaaaactc
    50281 gatgcagagc aaaacaagtt aatctggctg acgcccgcac cagcgaataa cacctggacc
    50341 atcgccgtgc gtcaggatgt ggcagagaaa aacaaactca cttcgcttgc tgacctgagt
    50401 cgttatctga aagagggcgg caccttcaaa ctggcagcct cggcagagtt tatcgaacgc
    50461 gccgatgcgt tacccgcgtt tgaaaaagcc tatggcttta agctcggtca ggatcagttg
    50521 ctgtcactgg ctggtggcga cacggcggtg acgatcaaag ccgctgccca gcaaacctct
    50581 ggcgttaatg ctgcaatggc ttacggcacc gacggcccgg tagcggcgct ggggctgcaa
    50641 accttaagcg atccgcaagg tgtgcaacct atctacgcgc ctgcaccagt ggtgcgtgag
    50701 tcggtgttga aggaatatcc gcaaatggca cagtggctac agccagtctt cgccagcctc
    50761 gatgcaaaaa cattgcagca gctgaatgcc agcattgcag tggaaggact ggatgccaaa
    50821 aaagtggctg ccgactacct gaaacaaaaa gggtggacga agtaatttcc cgtgacttat
    50881 ctacgtatta atcctgttct ggcgctgctg ctgttgctga cggcaatcgc agcagcgctg
    50941 ccgtttatca gctacgcgcc taatcgttta gtctcgggtg aagggcgaca tctctggcag
    51001 ctgtggccgc aaacgatttg gatgctggtg ggcgttggtt gcgcctggct gacagcctgt
    51061 tttattcccg ctaaaaaagg cagcattttt gcactcattc tggcgcaatt cgtcttcgta
    51121 ttgctggtgt ggggagcagg aaaggcggcg acccaactgg cgcaaaatgg cagtgcgctg
    51181 gcgcgtacca gcctcggcag tggtttctgg ctggccgcgg cgctggcatt gctggcctgt
    51241 agtgatgcca tccgccgaat ctccacgcat ccgctgtggc gctggttgtt gcatatgcag
    51301 attgccatta ttccgctgtg gttgctgtac tccggcacgc ttaacgatct ctcactaatg
    51361 aaagaatacg ccaaccgtca ggatgtgttt gacgacgcgc tggcacaaca tctgacgttg
    51421 ctgtttggtg cggtgctgcc tgcgttagtg attggtgtgc cgttgggcat ctggtgctac
    51481 ttttccactg ctcggcaggg ggcaattttt tctctgctca atgtcattca gaccgtgcct
    51541 tcggtggcgc tctttggcct gttgattgcg ccgcttgccg cgctggtgac ggcctttccg
    51601 tggctgggga agctcggcat agcaggaacc ggaatgacac ccgcactgat tgcgctggtg
    51661 ctctatgcct tgctgccgct ggtgcgcggc gtggtagtcg gcttgaacca gatcccgcgc
    51721 gatgtgctgg agagcgccag agcgatgggc atgagcgggg cgcggcgatt cctgcatgtt
    51781 cagttaccac tggcgttacc ggtatttctg cgcagcctgc gggtggtgat ggtgcaaact
    51841 gtaggtatgg cggtgattgc ggcgttaatc ggcgcaggcg gttttggtgc gctggttttc
    51901 caggggctgc taagcagcgc cattgattta gtgttgctgg gggtgatccc ggtaattgtt
    51961 ctggcggtgc ttaccgacgc gctgttcgat ttgcttatcg cactgctgaa ggtgaaacgt
    52021 aatgattgaa tttagccatg tcagcaaact gttcggcgca caaaaagccg ttaacgatct
    52081 caatctcaat tttcaggaag ggagtttttc ggtgctgatt ggcacatctg gctccggcaa
    52141 atccaccacc ctgaaaatga ttaaccgcct ggtggagcat gacagcggag tgatccgctt
    52201 tgccggagaa gaaattcgct cgctgccagt actggagttg cgccgccgga tgggctatgc
    52261 cattcaatct attggcctgt tcccccactg gagcgtggcg caaaacatcg ctaccgtgcc
    52321 gcaattacaa aaatggtcgc gggcgcggat tgacgatcgt atcgacgaat taatggcgct
    52381 actggggctg gagtcaaatt tgcgtgagcg ttatccgcat cagctttccg gtggtcagca
    52441 gcaacgtgtg ggagtggcgc gtgcactggc tgccgatccg caagtcttac tgatggatga
    52501 accttttggc gcactggacc cggtaacgcg cggcgcgttg caacaagaga tgacgcgcat
    52561 tcaccgtttg ctggggcgta ccattgtgct ggtcactcat gatattgatg aggcgctacg
    52621 gctggcagaa catctggtat tgatggatca cggtgaagta gtgcagcagg gcaatccgct
    52681 gacgatgctg actcgtccgg cgaatgattt tgtccgccag ttttttggac gtagtgaact
    52741 gggtgtgcgc ctgctttcgt tacgtagtgt ggcggattac gtgcgtcgcg aagaacgagc
    52801 agatggtgag gcactggcag aagagatgac gctacgcgat gcgctctctc tgtttgttgc
    52861 gcggggatgc gaggtgctgc cggtggtgaa catgcagggc cagccttgcg gcacgctgca
    52921 ttttcaggat ctgctggtgg aggcgtaagc gtatgaagat gttgcgcgat ccgctgttct
    52981 ggctcattgc tttgtttgtg gcactgattt tctggctgcc ttacagccag ccgctgtttg
    53041 ctgccttgtt cccacaactg ccacgacccg tttatcagca agaaagtttt gcagctctgg
    53101 cactggctca tttctggctg gtgggaattt cgagtttgtt tgcggtgatc attggcactg
    53161 gtgccggaat tgcagtcact cgcccgtggg gcgcggaatt tcgcccactg gtggaaacta
    53221 ttgccgccgt tgggcagact tttccgcctg tcgcagtgct ggcgattgcc gttccggtga
    53281 tcggctttgg tctgcaacca gcgattatcg ccttgatcct ttacggtgtg ctgcccgtcc
    53341 tgcaggcgac acttgccggg ctgggagcga ttgatgccag cgtgacagaa gttgcgaaag
    53401 gtatgggaat gagtcgtggt cagcgactgc gtaaggtcga gctaccgctg gcggctccgg
    53461 tgattctggc gggcgtgcga acttcggtga ttatcaacat tggtacggcg acgatcgcct
    53521 caacggtagg ggccagcacg ctgggtacgc ccatcatcat cgggcttagc ggatttaata
    53581 ccgcgtatgt gatccagggg gcgttactgg tggcactggc ggcgatcatc gcagaccgcc
    53641 tgtttgaaag gctggtgcag gcgcttagcc agcacgcaaa ataaaggtat aacctgcgag
    53701 catgacgcca ccaattccgc ctaacgccat aaacaggaac agggcgatga ccccaatttt
    53761 agctatgcgc ataatgcact ccttatgtta acgaaaggat tgtacagtaa agcgcatttg
    53821 ttaacgaatc attaaatgcc gagtgggaaa atatcatggc cttgttcctg ccaactggtg
    53881 agttgttgct gttgggcgga ggttcgattt tcaccgcacc acaccagcaa tgtacggcct
    53941 tcgaatagtt cagggcgtag ttgattgagc gagtgggcga ggacatcaat gcgccatcct
    54001 tgttgactgg caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca
    54061 accaccagcg catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag
    54121 ttgatcagta cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga
    54181 agccgtcggc gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga
    54241 ccgcgctctt tgatccacgt tcgcaggcta tgtagattgc cgctttgcag gtaagtcagt
    54301 aatgtttctt gctgatcgcg ccagccgttc tgcacatcaa cattttcatt actgagcagc
    54361 attttaactt tgctgacctg cacgccgttg tcgatccagc gtttgatctc gcggatccgg
    54421 tcaatatcgg catcgttgaa cagccgatga ccgccgtctg tccgttgcgg tttcagcaat
    54481 ccgtaacgcc tctgccacgc gcgtaacgtg acaggattaa tatcacaaag caacgccact
    54541 tcaccaattg tgtaaagcgc catcgtctca cccttgctcg cgaggtcccg gtttaacttt
    54601 agacgcagtt ttgcgaacca ggtagttttg cccgtttttt gtgcatctat agggtgattt
    54661 tatttttgcc aggcgatttt gagtgatcgt actcacgaat tctcattttt ctgcaagagt
    54721 tcaaagaaag ttaaacgcag gcaatgtatg ttacgcgttt taaagggaag tgtggtttgc
    54781 gggtatgtac gattttaatc tggtgttgct gctgcttcag cagatgtgcg tttttttagt
    54841 cattgcgtgg ttaatgagta aaacgccatt attcataccg ttaatgcagg tcacggttcg
    54901 tctgccgcat aaatttctct gctacatcgt cttttccatc ttctgcatca tgggcacctg
    54961 gtttgggttg cacattgacg attctattgc caatacccgt gcgataggcg cggtaatggg
    55021 cggcttactc ggcggtccgg tcgtcggtgg gctggttggt ctgaccggcg gcttacatcg
    55081 atattcgatg gggggcatga ccgcgctaag ttgcatgatc tcaaccatcg ttgaaggatt
    55141 gctcggcggc ctggtacaca gcatcctgat ccgtcgcggg cgcactgata aagtctttaa
    55201 ccccattacc gccggtgccg tcacgttcgt cgctgaaatg gtgcaaatgc tgatcatcct
    55261 tgcgatcgcc cgaccttatg aagatgcggt gcgtctggtg agtaatattg ctgcgccaat
    55321 gatggtcacc aataccgtcg gcgcggcgct gtttatgcgt atattgctcg ataaacgcgc
    55381 gatgtttgaa aaatacactt cggctttttc tgccactgcg ctgaaagtgg cagcctcgac
    55441 ggaaggcatt ttgcgacagg ggtttaacga agtgaacagc atgaaagtgg ctcaggtgct
    55501 gtatcaggag ctggatattg gtgcagtcgc gattaccgat cgagagaaat tgctggcctt
    55561 taccggaatt ggtgacgacc accatttacc cggcaaaccg atttcttcga cttacacctt
    55621 aaaagcgatt gaaaccggtg aagtggtcta cgctgatggc aacgaagtac cttatcgttg
    55681 ctctttgcat ccgcaatgca aactggggtc gacgctggta attccgttgc gtggcgaaaa
    55741 tcagcgcgtg atgggcacca tcaaattgta tgaagccaaa aaccgtttat tcagttcaat
    55801 aaaccgcacg ctgggcgagg ggattgcgca actgctttcg gcgcagattc ttgccgggca
    55861 atatgagcgg caaaaagcga tgctcaccca gtcagaaatc aaactgcttc acgcccaggt
    55921 gaatcctcat tttttgttta atgcgcttaa caccattaaa gcggtgatcc gccgcgacag
    55981 cgaacaggcc agccagctgg tgcagtatct ttccactttt ttccgcaaaa acttaaagcg
    56041 gccttcggag tttgttactc tcgccgacga aattgaacat gtgaacgctt atctgcaaat
    56101 tgaaaaggcg cgcttccagt cgcggttgca ggtcaacatt gctattccgc aagaattatc
    56161 ccagcagcaa ttgcccgcgt ttaccctgca accgatagtg gaaaacgcca ttaaacatgg
    56221 gacatcacaa ctgctggata cagggcgagt ggcaatcagc gcccgacgtg aggggcaaca
    56281 tttgatgctg gagatcgaag acaatgccgg tttgtatcaa ccggtaacca atgccagtgg
    56341 gctggggatg aatctggtgg ataagcgttt acgtgaacgg tttggcgatg actatgggat
    56401 aagcgtcgcc tgtgagcctg atagttacac ccgaataacg ttacgactac catggaggga
    56461 cgaggcatga ttaaagtctt aattgtcgat gatgaaccgt tagcacggga gaacctgcgc
    56521 gtatttttgc aggagcagag cgatattgaa atcgttggtg agtgttcaaa cgccgtggaa
    56581 gggatcggcg cggtgcataa actgcgcccg gatgtgctgt ttctcgatat ccagatgccg
    56641 cgcatcagtg gtctggaaat ggtggggatg ctcgacccgg aacatcgccc gtatattgtt
    56701 tttctcactg cgtttgacga atacgctatt aaagcctttg aagaacatgc ctttgattat
    56761 ctgctgaagc caattgatga agcgcgactg gagaaaacgc tggcgcgatt gcgtcaggag
    56821 cgcagcaagc aggatgtttc gctgttaccg gaaaatcaac aggcgctgaa atttatccct
    56881 tgtacggggc atagtcggat ttatttgctg caaatgaaag atgtggcatt tgtcagcagt
    56941 cggatgagcg gtgtctacgt taccagccac gaagggaaag agggctttac cgaattgaca
    57001 ttacgtaccc tggaaagtcg tacaccacta ctgcgctgcc atcgtcagta tctggttaac
    57061 ctcgcgcatt tacaggagat tcgtctggaa gataacggcc aggccgagtt gattttgcgt
    57121 aatggcttaa ccgtgccggt cagccgccgt tatctgaaaa gcttaaaaga ggcgattggc
    57181 ctgtaaaaga ctgctaaaat ggctttttgc ctcatcaaca cctgaaggcc tcatgctaag
    57241 taacgatatt ctgcgcagcg tgcgctacat tttgaaagcc aataataatg acctggtgcg
    57301 tattctggcg ctgggtaatg tcgaagccac cgcggaacag atcgccgtct ggctacgtaa
    57361 agaagacgaa gagggttttc agcgttgtcc ggacattgtt ttgtcgtcat tcctcaatgg
    57421 cctgatttat gaaaaacgcg gcaaggatga gtctgctccg gcactggagc cggaacgtcg
    57481 cattaataac aacatcgtgc tgaaaaaatt acgcatcgcg ttttcgctga aaaccgatga
    57541 cattctggcg atcctcaccg aacagcagtt ccgcgtttcg atgccggaaa ttacagcgat
    57601 gatgcgcgca ccggatcata aaaacttccg cgaatgcggc gatcaatttt tacgttattt
    57661 tctgcgtgga ctggcagcgc gccagcatgt gaagaaaagc taagacgggt atggcggcca
    57721 tgcgaaacat ggccgccgac agattatttc acttctttaa aaccagcggc tttcatcacc
    57781 agttccattt gcgccatagt gatacctttt ttggcatctt cagcagaaac gttgattcct
    57841 gaaataccct gcagggcttt aaaatccact ttttccatat cgatagtcac gttttcctgc
    57901 gcgtaggtat cggtataggt taatttttct tcaacacccg cgatgttttt gtatttggcg
    57961 cttaacggct caagtgccct ggcagcatct tctttggtgg ttgcaccaat ggaggcaaat
    58021 tgaattttgg tttcagaaga ttgcttaagc accttgtcac ctttgtagac ataggtaatg
    58081 gcaatttcag tgccgttcag attggcgctg aatttcttcg attcttcttt gtcaccgcag
    58141 ccagcaagag agaaaaccag aacagatgca acaacgaggg aaaacagctt attgaaagcc
    58201 tttatgtaaa actccatttt atttaatcaa gaaactggtg actctcacca ggggctatat
    58261 agaatatgcc taataccgtg gcgtgagcag tccggaactg gagtagaact cttagtaaaa
    58321 agcactattt catccttgtt gctgaagcat ggggaataat tgttcgcaaa gcaaaacacc
    58381 gttattcatt gcttctaccc gtgcctcgct ttctgtatta cgaaattgtg ccaacacatg
    58441 tgccagccga taaaaaccca ccgcggtgag gtcattcgcc agcaactctg cctgactaat
    58501 agcgctctgt tcctgatagc gccagccgtt atggagcagt tgaataagta acgcctggca
    58561 gcgcatcagc aactgatgag cagtagacgg cacaggcaga acgctggcag aaggtagtga
    58621 taccaccaca ggcgcagttt ctgcgtccag cgcccaggca cgagtctttg tcatcattac
    58681 ctgtggttcc agtgtcaatt gcccatcaac aaaactgaca aagccagaaa ccagatacac
    58741 ggggtcgtct gtttgttgca aaagcgccgc catgcgttca acggcataag gcgcgctggc
    58801 tgaggctggc agtgataacg tcagcagatt atcttcacct tcgccgctga ttacctgcgc
    58861 atccagcgtc tggcggctgc tatcccaacc gagcgaaata cactcagcga ccggcagaat
    58921 aaataagtta tcgacctgat taagaggccg tatgcaggcg gggggacgct ggcgtaaata
    58981 ttcccgcaaa gccacaatgc ccggctggcg taacggcgcg ctcaacattt gccaggcatc
    59041 aggcgacagc ggcacaacgc tgcttaagcg gttgcgggta gctaacagca gctcgccatc
    59101 ggcactgcgt tttgctgctt gtgaaacaat ttgcccgccc gccagtgcgc cagcctgaaa
    59161 actaaacagc cgacgcgtag ctgccggtga gttttcctgt tcacttcgcg gccaactgcg
    59221 cgaaaggtgc aaaatactgc cggtgtcggg atcggtaaac cagatgcgta aaccataatg
    59281 ctcaatatcc tgccagcaac gcatacctaa agacaccagc cgcagatgat caagctttgc
    59341 ttctccggca atgcctgcgc caacgaccgt gcgccacggc atgggaggaa tttcaccaac
    59401 actgtcgcgc tgcgccattt cgtgtgcgca atttaatcga ctgtttaatg cggcaagctg
    59461 acacaagcat tctccggcat gataatggct tgcgcgggcg tggaaggcat caacgctggc
    59521 gcgcagttgc cgtagcgatt cactcaccca tcgccagttg cagcgttccg ccgcttgctg
    59581 cgcgcggctg aaagcggcct cgtagtgaat aagcggctgg ctgatgccgc caagccataa
    59641 tgcctggctt aattgctgaa catattgacg acacgtttgg ccttcttcgc tggcaaacgg
    59701 atcgtcagat gatgtgacgt gttcgctgcg catctgccag attaaatggt taaattctgc
    59761 ttgctgcgct ttggcctcga cgaaggcctg taccgccagt acgatatgtt cgcaaagagt
    59821 gccttcaata caatcacaac gggcgaaacg aatgctgctg cgggaataaa aacgcacatc
    59881 gctcatcggt aagcgggcag agggaatttc gcccggcgta cagaacaact caatggtgat
    59941 gcctttagcg accagcgcct gcgcgcgttt gcgggtggca tcgggcaggg tagccagttc
    60001 ttccagccag attgccggat cccactcttc ttctttttcc gcaggctggg cggtagcaca
    60061 aagtcgttga taacttaaca ccagcatcac gcgatgacgg cacatgccgc tggccccaca
    60121 ggtgcactga gcctctttca gtgcctggcc gttcgccagc tgggtacgga caccgtcact
    60181 gaaggtggcg attaaagcgc cgttctcatg gctgatttcc gggacgttgc cattttccag
    60241 ttccttaagg ctgcgcttaa caaaaccggc attgcttaac gccgtcagtg cctgtggtgt
    60301 cagttctaat aattccggac gtagtgaatt catgactgaa ggttctctgc aagccatgat
    60361 gccagctcgc ccggcgtcat ggcggctatt tgtgcgccga cattaaccag cgcctgggcc
    60421 gtatcgcggt catagcaagg cgttgctgtg ctatcgagcg ctgccagtcc cagcactttg
    60481 atgccgctct ggacacactt tttcacctga tgcgtcagca atgatgatga acccccttca
    60541 taaaaatcgc tcacgaggat aatgacgctt tttgctggtt gttcaataag ttgccgacca
    60601 tactccacgg cactggcgat attggttccg ccgcccagtt gtactttcat taatagttct
    60661 accggatcgg caacgtctgc cgtgagatca acgacgcttg tgtcaaacgc caccagatgg
    60721 gtacgaatgc cgggtaactg ccacaaacag gccgccatca ccgcagagtg gatcaccgaa
    60781 tcgaccatcg agccgctttg atcaaccagt aagaccagtt gccattgttc gctttggcgt
    60841 ttaatgcggc tgttaaatcg gggggattcg atatacaact tgccgtgttg cgggtgccag
    60901 tgttgcaggt tggcgcgcag agtacttttg aaatcaaagt ttcgcgccag tggaataaat
    60961 gagcggcgac ggcgatcgcg gacaccagaa aaagcctgac gaacttcctt tgccagtcga
    61021 gccataattt cttcaacaac ctggcgcact atccggcggg cggcagccag tacttcggga
    61081 ttcatcagat gtttggtgtg caaaacggcg cgtagcaggc tttcagaagg ctgcatacgt
    61141 tccagcacgt cgagattcgt caccacatct tcaatgccgt agcgcagtac ggcatcgctt
    61201 tccagccgct caatcacctg ttgcggaaac agcgtgtgaa tactgttgat ccactcaggg
    61261 gtggtgagat ttgagccacc taatccaccg gagcgttcac cacgctggag ccgttcagga
    61321 tcgcgcccat acagccactc caacgcgtga tctatctgcc gggcattgcc atccagccca
    61381 caaagtgtcg tttctgccgc ttcgccaaga attaatcgcc agcgttgtag ctcacgggtg
    61441 gtcagaagat cgttcagttc agacatggaa aactccccag tgttgcagcg atgccagtgc
    61501 ctgctgttcg agttgttgat gatatgcaat ggcttgtggt ggacaatgca acggcatttg
    61561 cagtgccgaa acgggaagtt gcgccagttg ataatgttca agcacctgat gcgccagtgt
    61621 cccgcgttct cgtggcggta gccaggccat cgccgcgcgc aaatcgggca gggcgttaat
    61681 aaaatcgtcg tcactcagtt gatttaaatg actgctgaaa ccggcgataa atgccggctg
    61741 acaggccagt tgatgacggg ctaatgccag caaaccgtgc agcgcctcgc cggattgtgc
    61801 cggggataac tgcgccagca tcatcagcgc cgcttcggca ctggcgttgg gatgttccag
    61861 acgcattagt gcgccaagag ccgcgccgcg atccagcgcc ggggcatgaa tcgcctgact
    61921 gcgtcgctcc agtaaggcta ccgccgcaga aagagaaacg ccgggtaaat taacgccgtt
    61981 atgtagatcg cgcaggatat ggcaaagcgc ctgccagcta tgcaggtgag cgtgaaactc
    62041 cttttcatcc ggtctgccgt tagattcaca cagccacagc gtgcgatcga tagccgcgca
    62101 taacgtcgtt tgtaatatct gcgcgccttg cataccgcta atttcatcca gccgccataa
    62161 ggcatatagc acttccagcg cctggcccat ttcggcaaat tgattttcct gggcgattaa
    62221 taatgtgagt tgctccagta attgttgact gaaggacgcc agacccgcta acgccgcttg
    62281 gctaagacat gtggtgatac tgccgattcc acccgcgtcc agcatatcgg cttctaattt
    62341 atggcgtgcg gcttcctgga gtgtggcacc aaagcaggcg gcctcaatca atgccgcatg
    62401 ctggctaagc gggcgggtta atttccagtg ttcttcaccg ttgcctgcaa gtgtcagtgt
    62461 gcttccctgc tggcgcacaa tccctgggat ctccagtatt gccagccgat gtaacacctg
    62521 actttgcgct agtccattgg ggttaaagcg atttaaggta agctccgccg gaagggagat
    62581 tgcggtacgt tccagttcgc aggtgacatc ttttggcaga ggaggttgtg gcgtagaagg
    62641 ggcaagttta ccgaatccgt cacccgctaa tgtgtctatc aacgttagca gaatcggatc
    62701 ggtgtcggga tgaataacgc cgcgatagct ccacggcaac ggcgcattca gggcttcttt
    62761 aatcagcgat cctgctatgg catccagcca gtcagtgcgt aacggtagtg tatgaccgcg
    62821 caactgtgcc agtgccattg catgcagatg tgcggcagcc atatccgcgg tcgaagcagg
    62881 caacttgtgc tggcgcaaac gggtgagaac cgtttttagc agttgttctc ccgcctgctg
    62941 taagccccac tgccagcacc agttttgcca gaccggggca ggcattccgg aaaggtatcc
    63001 tgccagcaca tcaaggcgct tttcactgta gggggtgaga taacaacctg taacggcatc
    63061 tgccagcgac ggcaattctg gcgtgttaat ttcctgcggg cattcgcgcc acatttttgc
    63121 cagtaccgga gcgtgccagc caccgcagac aactaacacg tcgccattat tttgctgcat
    63181 cgcccatcca atccagcggg ccataaaggc ttcgcgcaga cgattgagag catctccggg
    63241 ggagtcgccc cgcagttggg caaaatagtg cgccagtgca gagggtaatg cagtttgctg
    63301 gctttcatct tcgaacaagt gatcccacag ggtgtcgctg ttatccatgc gggtggcacg
    63361 cagcagcaag gcctggcttt cctcttgcgt atcaggcgaa tcgtccactt cttcactctg
    63421 cgcccagcaa ggcaaatcga tgaagtaagt ttgtgcctga atacgacgtg cggcttgtag
    63481 cgcctgccac tccggcgaaa attcagcaaa tggcgtccag gcaccacgcc cgggggctgc
    63541 accgtcctga tactggcaat aactgtaaat agctaccgga agctggtggg ctaaaaacag
    63601 ttcgtctacc cggtcattaa aatcagccgg gccttcaatc aatacgtatc gtggccgctg
    63661 gctttcgatt aacgatttca ccagccgggc gcaggccgga ctttgatgcc ggatgccgac
    63721 aattaacggc tcgctcatgc atctttcctc acggcaggcg gtggcgggct tgataataag
    63781 cctgccagtg agcttcttta tgtgtggcga ctcgttgttc gaagtaacgg cgcagacgag
    63841 cgcgatcttc ttcgttatct ttgacgatgg ttccggcaat gcagtccacc aggtcggctg
    63901 gctcacctgc gcgattcgct aaaaaccagg cgcggacgcc tacggcgtgg gccacgttca
    63961 cggcttcagc ggtggacata atcgctgtta gtgtatccat tgacgttttc ttttcgccgt
    64021 tggcacgcag atcgcggaag gtgcggacca gtaactccag taccgcgtca ggcactttat
    64081 gcggaatacc gctatgggcc agcaaacgcg ccgaagcact ggcgacaagc tccagctcct
    64141 gggcgaaatc cataatcgga aagacagttt caaaatcgaa gcgacgcttt agcgcagcac
    64201 tcatttcatt gacgccgcga tcgcgggtat tggcagtggc aataatatta aagccttctc
    64261 gcgcatagag ctgactggct tcaccagtga gttccggcac cgtcatcacc ctgtcagaaa
    64321 gcatacctaa cagacagtct tgcacttcca gcggtgtacg cgttatctct tcaaaacgga
    64381 cgattttgcc gtcgcgcatt ccctggtaaa gcggggcagg gaccagcgct tccgttgatg
    64441 ggccatgatt gataagcaga gcgtagttcc agccgtactt gatttgatct tcagtggtag
    64501 atgcgccgcc ctggatggtt aacccggcat cgccgctaat agcggttgcc agtaattcag
    64561 acaggagaga ttttgcggtg ccaggttccc ccaccaacat caaaccgcgt ccggtggcaa
    64621 gagtgactaa catacgttcg acagcggagg gactaatcac cactttacgg ctaatatttt
    64681 gcgcgctatc gccaaggata aaagcacggg ccgcaggcaa acttaactgc caaccgggcg
    64741 ggcaaggtgc gttatcattt tgttttaatt ttgccagttc atcggcgtat aacacagcag
    64801 caggcggacg ctgcagatga ttattctgcg gtgacatata ttcatccctg aaatgtatta
    64861 atgaaataaa cgatatttta cgcgcaataa atgtttggcg cattagttgt ttgagcggaa
    64921 aatatctgga aagcagatca cagtaaataa aaaaacctgc tggtgaggca ggttctttta
    64981 tttactacag tgacgctaac ttgctgattg tgcaacattt cgtaccaacg aataaattat
    65041 aaccatatcc acactccatt gcaattcagt gaatgagatt tataatcctg cccgttggga
    65101 ggttgtgccg gatcggacca attttaagtc ctggctgctg gacgggtggt gccgcaggcg
    65161 gtgccctgat ccggagagca acgtttcgta ccaaca
//
LOCUS       DAOFQD010000011        59024 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.011, whole genome shotgun sequence.
ACCESSION   DAOFQD010000011 DAOFQD010000000
VERSION     DAOFQD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.011"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            126..884
                     /locus_tag="QBF46_000724"
     CDS             126..884
                     /locus_tag="QBF46_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDQ6672953.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(937..1044)
                     /locus_tag="QBF46_000725"
     CDS             complement(937..1044)
                     /locus_tag="QBF46_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6672954.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1220..3118)
                     /gene="espL1"
                     /locus_tag="QBF46_000726"
     CDS             complement(1220..3118)
                     /gene="espL1"
                     /locus_tag="QBF46_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HDQ6672955.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSNIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYRQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLHILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            3641..5569
                     /gene="thrS"
                     /locus_tag="QBF46_000727"
     CDS             3641..5569
                     /gene="thrS"
                     /locus_tag="QBF46_000727"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDQ6672956.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            5573..6115
                     /gene="infC"
                     /locus_tag="QBF46_000728"
     CDS             5573..6115
                     /gene="infC"
                     /locus_tag="QBF46_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDQ6672957.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            6212..6409
                     /gene="rpmI"
                     /locus_tag="QBF46_000729"
     CDS             6212..6409
                     /gene="rpmI"
                     /locus_tag="QBF46_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDQ6672958.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            6462..6818
                     /gene="rplT"
                     /locus_tag="QBF46_000730"
     CDS             6462..6818
                     /gene="rplT"
                     /locus_tag="QBF46_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDQ6672959.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    6935..7059
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6941..6985
                     /gene="pheM"
                     /locus_tag="QBF46_000731"
     CDS             6941..6985
                     /gene="pheM"
                     /locus_tag="QBF46_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDQ6672960.1"
                     /translation="MNAAIFRFFFYFST"
     gene            7269..8252
                     /gene="pheS"
                     /locus_tag="QBF46_000732"
     CDS             7269..8252
                     /gene="pheS"
                     /locus_tag="QBF46_000732"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDQ6672961.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            8267..10654
                     /gene="pheT"
                     /locus_tag="QBF46_000733"
     CDS             8267..10654
                     /gene="pheT"
                     /locus_tag="QBF46_000733"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDQ6672962.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            10659..10958
                     /gene="ihfA"
                     /locus_tag="QBF46_000734"
     CDS             10659..10958
                     /gene="ihfA"
                     /locus_tag="QBF46_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDQ6672963.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            11059..12039
                     /gene="btuC"
                     /locus_tag="QBF46_000735"
     CDS             11059..12039
                     /gene="btuC"
                     /locus_tag="QBF46_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDQ6672964.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            12102..12653
                     /gene="btuE"
                     /locus_tag="QBF46_000736"
     CDS             12102..12653
                     /gene="btuE"
                     /locus_tag="QBF46_000736"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDQ6672965.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            12653..13402
                     /gene="btuD"
                     /locus_tag="QBF46_000737"
     CDS             12653..13402
                     /gene="btuD"
                     /locus_tag="QBF46_000737"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDQ6672966.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            13480..13944
                     /locus_tag="QBF46_000738"
     CDS             13480..13944
                     /locus_tag="QBF46_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HDQ6672967.1"
                     /translation="MRFCLILITALLLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            14191..14903
                     /gene="ydiV"
                     /locus_tag="QBF46_000739"
                     /pseudo
     CDS             14191..14903
                     /gene="ydiV"
                     /locus_tag="QBF46_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            14966..16402
                     /locus_tag="QBF46_000740"
     CDS             14966..16402
                     /locus_tag="QBF46_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDQ6672968.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGERMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(16406..16597)
                     /gene="hemP"
                     /locus_tag="QBF46_000741"
     CDS             complement(16406..16597)
                     /gene="hemP"
                     /locus_tag="QBF46_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDQ6672969.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(16729..17775)
                     /gene="aroH"
                     /locus_tag="QBF46_000742"
     CDS             complement(16729..17775)
                     /gene="aroH"
                     /locus_tag="QBF46_000742"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDQ6672970.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(17932..18765)
                     /gene="ppsR"
                     /locus_tag="QBF46_000743"
     CDS             complement(17932..18765)
                     /gene="ppsR"
                     /locus_tag="QBF46_000743"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDQ6672971.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            19098..21476
                     /gene="ppsA"
                     /locus_tag="QBF46_000744"
     CDS             19098..21476
                     /gene="ppsA"
                     /locus_tag="QBF46_000744"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDQ6672972.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(21533..23179)
                     /gene="fadK"
                     /locus_tag="QBF46_000745"
     CDS             complement(21533..23179)
                     /gene="fadK"
                     /locus_tag="QBF46_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDQ6672973.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(23236..23529)
                     /locus_tag="QBF46_000746"
     CDS             complement(23236..23529)
                     /locus_tag="QBF46_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDQ6672974.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAENPDINEFHKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(23526..24815)
                     /locus_tag="QBF46_000747"
     CDS             complement(23526..24815)
                     /locus_tag="QBF46_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDQ6672975.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRMDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLEMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(24871..25809)
                     /locus_tag="QBF46_000748"
     CDS             complement(24871..25809)
                     /locus_tag="QBF46_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDQ6672976.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(25829..26593)
                     /locus_tag="QBF46_000749"
     CDS             complement(25829..26593)
                     /locus_tag="QBF46_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDQ6672977.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            26909..27820
                     /locus_tag="QBF46_000750"
     CDS             26909..27820
                     /locus_tag="QBF46_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDQ6672978.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYCEKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSVFPQPVSLVG"
     gene            complement(27865..29016)
                     /locus_tag="QBF46_000751"
     CDS             complement(27865..29016)
                     /locus_tag="QBF46_000751"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDQ6672979.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(29030..30625)
                     /locus_tag="QBF46_000752"
     CDS             complement(29030..30625)
                     /locus_tag="QBF46_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDQ6672980.1"
                     /translation="MKPVKPLRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQSGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSIKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(30771..31529)
                     /gene="aroD"
                     /locus_tag="QBF46_000753"
     CDS             complement(30771..31529)
                     /gene="aroD"
                     /locus_tag="QBF46_000753"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDQ6672981.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPETPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVTYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(31560..32426)
                     /gene="ydiB"
                     /locus_tag="QBF46_000754"
     CDS             complement(31560..32426)
                     /gene="ydiB"
                     /locus_tag="QBF46_000754"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDQ6672982.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(32438..33703)
                     /locus_tag="QBF46_000755"
     CDS             complement(32438..33703)
                     /locus_tag="QBF46_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6672983.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFCTRLNTIKHRG"
     gene            complement(33930..35144)
                     /locus_tag="QBF46_000756"
     CDS             complement(33930..35144)
                     /locus_tag="QBF46_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6672984.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIATIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(35244..35600)
                     /locus_tag="QBF46_000757"
     CDS             complement(35244..35600)
                     /locus_tag="QBF46_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDQ6672985.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(35736..35843)
                     /gene="rprA"
                     /locus_tag="QBF46_000758"
     ncRNA           complement(35736..35843)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="QBF46_000758"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(36029..37141)
                     /gene="ydiK"
                     /locus_tag="QBF46_000759"
     CDS             complement(36029..37141)
                     /gene="ydiK"
                     /locus_tag="QBF46_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDQ6672986.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            37530..40586
                     /locus_tag="QBF46_000760"
     CDS             37530..40586
                     /locus_tag="QBF46_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDQ6672987.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGIFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            40583..40993
                     /gene="menI"
                     /locus_tag="QBF46_000761"
     CDS             40583..40993
                     /gene="menI"
                     /locus_tag="QBF46_000761"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDQ6672988.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            41093..41281
                     /locus_tag="QBF46_000762"
     CDS             41093..41281
                     /locus_tag="QBF46_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDQ6672989.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            41829..42197
                     /gene="sufA"
                     /locus_tag="QBF46_000763"
     CDS             41829..42197
                     /gene="sufA"
                     /locus_tag="QBF46_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDQ6672990.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGIVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            42206..43693
                     /gene="sufB"
                     /locus_tag="QBF46_000764"
     CDS             42206..43693
                     /gene="sufB"
                     /locus_tag="QBF46_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDQ6672991.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            43703..44449
                     /gene="sufC"
                     /locus_tag="QBF46_000765"
     CDS             43703..44449
                     /gene="sufC"
                     /locus_tag="QBF46_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDQ6672992.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPGLCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            44424..45695
                     /gene="sufD"
                     /locus_tag="QBF46_000766"
     CDS             44424..45695
                     /gene="sufD"
                     /locus_tag="QBF46_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDQ6672993.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGVKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGVVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            45692..46912
                     /gene="sufS"
                     /locus_tag="QBF46_000767"
     CDS             45692..46912
                     /gene="sufS"
                     /locus_tag="QBF46_000767"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDQ6672994.1"
                     /translation="MIFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            46925..47341
                     /gene="sufE"
                     /locus_tag="QBF46_000768"
     CDS             46925..47341
                     /gene="sufE"
                     /locus_tag="QBF46_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDQ6672995.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRVIRAKAAALS"
     gene            47490..48494
                     /gene="ldtE"
                     /locus_tag="QBF46_000769"
     CDS             47490..48494
                     /gene="ldtE"
                     /locus_tag="QBF46_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDQ6672996.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNALDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(48558..48794)
                     /gene="lpp"
                     /locus_tag="QBF46_000770"
     CDS             complement(48558..48794)
                     /gene="lpp"
                     /locus_tag="QBF46_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDQ6672997.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(49105..50517)
                     /gene="pykF"
                     /locus_tag="QBF46_000771"
     CDS             complement(49105..50517)
                     /gene="pykF"
                     /locus_tag="QBF46_000771"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDQ6672998.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            51073..51282
                     /gene="fumD"
                     /locus_tag="QBF46_000772"
     CDS             51073..51282
                     /gene="fumD"
                     /locus_tag="QBF46_000772"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDQ6672999.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            51737..52363
                     /locus_tag="QBF46_000773"
     CDS             51737..52363
                     /locus_tag="QBF46_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDQ6673000.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            52384..54486
                     /locus_tag="QBF46_000774"
     CDS             52384..54486
                     /locus_tag="QBF46_000774"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDQ6673001.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEANFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            54499..55137
                     /locus_tag="QBF46_000775"
     CDS             54499..55137
                     /locus_tag="QBF46_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDQ6673002.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYLVAEEELSVLLESIKQNGDYADIACLTGSKDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            55201..55869
                     /locus_tag="QBF46_000776"
     CDS             55201..55869
                     /locus_tag="QBF46_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDQ6673003.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            55866..56651
                     /locus_tag="QBF46_000777"
     CDS             55866..56651
                     /locus_tag="QBF46_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDQ6673004.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVALHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            56655..57467
                     /locus_tag="QBF46_000778"
     CDS             56655..57467
                     /locus_tag="QBF46_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673005.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGARPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRARVRYRLNEQHQFVEC"
     gene            complement(57479..>59024)
                     /locus_tag="QBF46_000779"
     CDS             complement(57479..>59024)
                     /locus_tag="QBF46_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDQ6673006.1"
                     /translation="LQQQTPLSISIFEQADEAGVGMPYSDEENSKMMLANIASIEIPP
                     IYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILLGEYFRDQFLRLVDQARKQKF
                     AVAVYESCQVTDLQITNVGVMLATNQDLPSETFDLAVIATGHVWPDEEEATRTYFPSP
                     WSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFIEDDKQHVVFHRDNASEKLNI
                     TLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKGEEGLLDRVFRLIVEEIKFAD
                     PDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNLQEVERNKREKHTVPWRYVIL
                     RLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPSESIRRLLALREAGIIHILAL
                     GEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKVKDIPFHGLREQLQKTGDEIP
                     DVGEDYTLQQLEDIRGRVAFGALPWLMHDQPFVQGLTACAEIGEAMARAVVKPASRAR
                     RRLSFD"
BASE COUNT        15032 a        14008 c        15124 g        14860 t
ORIGIN      
        1 tacattgcta agttattcgc gcctttttat aattcgttac ctttccaggg acgctaatct
       61 ttgatcctta agaaagtttc cgggaccgcg atggtctctt tttttgttgt acggactctt
      121 gataaatgaa gcttttgaag acagttcctg ccatcgttat gttggcgggg ggcatgtttg
      181 cgtcactgaa tgcagctgcc gatgattccg tttttactgt catggacgat cctgccagcg
      241 ctaaaaaacc tttcgaaggt aatctgaacg caggatatct tgcacaatca ggcaatacga
      301 aaagctcctc acttacggct gacaccacca tgacctggta tggacaaacc actgcctggt
      361 cgctgtgggg gaacgccagt aatacttctt ctaatgatga gcgttcttca gaaaaatatg
      421 cggcgggcgg gcgtagccgt tttaatttaa ctgattatga ctatttattt ggtcaggcaa
      481 gctggctgac agaccgttat aacggctatc gagagcgcga tgtgttgacc gcgggttatg
      541 gtcgtcagtt tttgaatggc ccggtacaca gtttccgctt tgaattcggt ccaggtgtgc
      601 gttacgacaa atataccgac aatgccagtg aaactcagcc gctgggctat gcctccggtg
      661 cttatgcatg gcagctgact gacaacgcta aatttacgca gggtgtttcc gtctttggcg
      721 cggaagacac aacgctgaac tccgaaagcg cattaaatgt tgctattaat gaacattttg
      781 gactgaaggt ggcttacaac gtcacatgga actctgaacc tccagagtct gcaccggaac
      841 ataccgaccg tcggacaacc ttatcgctgg gctacagcat gtaaaattat ctgccggacc
      901 gtgttatgcg gtctggctgc tttcgtttgt cactgctcag ccccaaatca ttgctgccca
      961 acgtagcaac aacatagcgc cacagattgc cagtagcacc accaccatct tccagtgttt
     1021 tttatcaaag cgtttagtcg gcataccagt gtcctgcata tctcttgtgc gtgataacgg
     1081 agtgtaacaa aagggacagg catcaaggga ttttttgtta ttacagccgt cgggtggcgt
     1141 tgtgcacatg gtcactgttt ctgcgtttta tgaaaacaat aaaattatgt atcgatatac
     1201 aaatatcata aatagctggt tataaatacg tctttagttc atcagcacta aaactcaggc
     1261 tttggctgat gacatttata gcagcataat atgtttctac agttttataa tgattatgat
     1321 gaatggctat atgaacagct gacatgttgt catgattttt agcagccaac agtgtaaata
     1381 actgatgttg actaaaagaa tgtttttttg caaaagcacc caacgtcgat atataagata
     1441 acacgacgtc ttcattaccc ttgctcatgg cgatgtataa tgcaggggtt ccctgtgctg
     1501 tagcgccttt taataaatcc atgatgttag ctttgctcaa cttgtattta aaggcgatgc
     1561 cattaatttt agagaggaac cgtgtgacac acaaagggtg attattttcc attgcggcgt
     1621 ataatcctgg ctcaccatca tgtattttca tggctaaaat actcatcacc agttttttgt
     1681 ttaaaagctc ttgtgctata ggagtggtta atgcattcag aaagatatcg attatttcat
     1741 cgaatccata caacatggag atataaagtc caggtgtttc gttagcactt ttagtttgca
     1801 acaatttaac cagattatct tcatgaatga tgtgattatc taccagtgac tgaatttctt
     1861 gcataaatat ttttacgttg tcagtatgtc cgttcatcat agccaaaaat aaagcaggtg
     1921 taccaacgct attctgcgca gttattattt caatgatttc tttttcaggg agtatgtgca
     1981 actgtctgaa tagtgtaata agctgttgag tctggcctgt ggccattatt aacaacattg
     2041 ccgatggatg tataggggca aatggcattc gtgtcgcgat acccgtgaaa atagcccctg
     2101 gaactccctc aggtaatagg ggcagaatag caatagcaca gttgcgaaca tacctatcag
     2161 cccaggtctg agcatcggga cctgaactga tgaacttatc aagcgagaga aagccccgcg
     2221 ctgtcctgtg ggtgcctctg acggcaacgt tggtatcgtt cgggtcgtaa acggacacta
     2281 catagtttaa ttgcctatac gctgactgct ttattcgtag ccttactgac aaagtatgat
     2341 tgtctatata taaaatcgca gacatatgac tgacattgtt tgccatcatt cgacagaaga
     2401 aaatatctaa agcgaagcca aaatccttac tcgcgataac gcgactccct gcaggaggat
     2461 ggcgaagtaa tgctctgaac tccgcgtccc ggactaaggg gacatgctgt tgtatggatt
     2521 caacggagga gtaatgttta atcaggttga ccttgccttt tccatttctc aatgaatcaa
     2581 gtgcgtattg caatgacaga tggcgacaaa aaatagcgtc agaaggagat tgcgaaaaaa
     2641 atgcactacc attaagattt actgttggtt gtatagtttt ttctctatgg atttttttag
     2701 acagtttcat acaatggtta cgattttcgg cagttaactg ttcctgttca atataatcca
     2761 gtatgcttcg caaatggtta gctttaatag gcgtggtttc cagtaataag gaaatgattt
     2821 cttttttata gtgtaatgta atgagtatat tttctgcatg gatgggtgaa gtggcaaagt
     2881 taatggtaaa aagggcagtg gaataacaat tttgtgatat ttttcgacag tttgcggtaa
     2941 attcctgagt aacacagtat gacagcatcg aacttaagtc agaggtatca agtagggtga
     3001 tgagttggcg cgcaaattga ttaataggct ctgatgaagg cgttggtatg ttgctatcga
     3061 tgtgcattct atgaggagac ttataatgag ttctgataat aatgtcgttt tgactcataa
     3121 tctattcgcc ttatatatgt ctggaggttt tgaggatgat gattaattat tatcgccaga
     3181 acgaaatgtg cacgaaaaat tgctataaga attgtgtcac ttttatcatt gtgatatgac
     3241 cagttcgtca ttaatctcat gatcatttaa atagtaccgc caaatcatgc aacgaacttt
     3301 gctggattca gtttatgctg ctgtaaatcc gctcgcagta aacctttcag acgctccgtg
     3361 atgttatcag ttgttcttta agcgttttgc tggtgtactc actacaaacg aattgcgaat
     3421 cgaatcaatg tgaaacggaa aggtacaatc tccctttttt tagtgttaac atcgttcaac
     3481 cgggtggaag gtgagaggtt aatgtctttc atttgtgtac ctgattgtga accaatttag
     3541 catttgttgg ctagatggtt tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt
     3601 gatctttcgt gtgggtcacc actgcaaata aggatataaa atgcctgtta taactcttcc
     3661 tgatggcagc caacgccatt acgatcacgc tgtaagcccc atggatgttg cgctggacat
     3721 tggtccaggt ctggcgaaag cctgtatcgc agggcgcgtt aatggcgaac tggttgatgc
     3781 ttgcgatctg attgaaaacg acgcacaact gtcgatcatt accgcgaaag acgaagaagg
     3841 tctggagatc attcgtcact cctgtgcgca cctgttaggg cacgcgatta aacaactttg
     3901 gccgcatacc aaaatggcaa tcggcccggt tattgacaac ggtttttatt acgacgttga
     3961 tcttgaccgc acgttaaccc aggaagatgt cgaagcactc gagaagcgga tgcatgagct
     4021 tgctgagaaa aactacgacg tcattaagaa gaaagtcagc tggcacgaag cgcgtgaaac
     4081 tttcgccaac cgtggggaga gctacaaagt ctccattctt gacgaaaaca tcgcccatga
     4141 tgacaagcca ggtctgtact tccatgaaga atatgtcgat atgtgccgcg gtccgcacgt
     4201 accgaacatg cgtttctgcc atcatttcaa actaatgaaa acggcagggg cttactggcg
     4261 tggcgacagc aacaacaaaa tgttgcaacg tatttacggt acggcgtggg cagacaaaaa
     4321 agcacttaac gcttacctgc agcgcctgga agaagccgcg aaacgcgacc accgtaaaat
     4381 cggtaaacag ctcgacctgt accatatgca ggaagaagcg ccgggtatgg tattctggca
     4441 caacgacggc tggaccatct tccgtgaact ggaagtgttt gttcgttcta aactgaaaga
     4501 gtaccagtat caggaagtta aaggtccgtt catgatggac cgtgtcctgt gggaaaaaac
     4561 cggtcactgg gacaactaca aagatgcaat gttcaccacg tcttctgaga accgtgaata
     4621 ctgcattaag ccgatgaact gcccgggtca cgtacaaatt ttcaaccagg ggctgaagtc
     4681 ttatcgcgat ctgccgctgc gtatggccga gtttggtagc tgccaccgta acgagccgtc
     4741 aggttcgctg catggcctga tgcgcgtgcg tggatttacc caggatgacg cgcatatctt
     4801 ctgtactgaa gaacaaattc gcgatgaagt taacggatgt atccgtttag tctatgatat
     4861 gtacagcact tttggcttcg agaagatcgt cgtcaaactc tccactcgtc ctgaaaaacg
     4921 tattggcagc gacgaaatgt gggatcgtgc tgaggcggac ctggcggttg cgctggaaga
     4981 aaacaacatc ccgtttgaat atcaactggg tgaaggcgct ttctacggtc cgaaaattga
     5041 atttaccctg tatgactgcc tcgatcgtgc atggcagtgc ggtacagtac agctggactt
     5101 ctctttgccg tctcgtctga gcgcttctta tgtaggcgaa gacaatgaac gtaaagtacc
     5161 ggtaatgatt caccgcgcaa ttctggggtc gatggaacgt ttcatcggta tcctgaccga
     5221 agagttcgct ggtttcttcc cgacctggct tgcgccggtt caggttgtta tcatgaatat
     5281 taccgattca cagtctgaat acgttaacga attgacgcaa aaactatcaa atgcgggcat
     5341 tcgtgttaaa gcagacttga gaaatgagaa gattggcttt aaaatccgcg agcacacttt
     5401 gcgtcgcgtc ccatatatgc tggtctgtgg tgataaagag gtggaatcag gcaaagttgc
     5461 cgttcgcacc cgccgtggta aagacctggg aagcatggac gtaaatgaag tgatcgagaa
     5521 gctgcaacaa gagattcgca gccgcagtct taaacaattg gaggaataag gtattaaagg
     5581 cggaaaacga gttcaaacgg cgcgccctaa ccgtatcaat ggcgaaattc gcgcccagga
     5641 agttcgctta acaggtctgg aaggcgagca gcttggtatt gtgagtctga gagaagctct
     5701 ggagaaagca gaagaagccg gagtagactt agtcgagatc agccctaacg ccgagccgcc
     5761 ggtttgtcgt ataatggatt acggcaaatt cctctatgaa aagagcaagt cttctaagga
     5821 acagaagaaa aagcaaaaag ttatccaggt taaggaaatt aaattccgtc ctggtacaga
     5881 tgaaggcgac tatcaggtaa aactccgcag cctgattcgc tttctcgaag agggtgataa
     5941 agccaaaatc acgctgcgtt tccgcggtcg tgagatggcg caccagcaaa tcggtatgga
     6001 agtgcttaat cgcgtgaaag acgatttgca agaactggca gtggtcgaat ccttcccaac
     6061 gaagatcgaa ggccgccaga tgatcatggt gctcgctcct aagaagaaac agtaaggcct
     6121 tcaagtagca ctgtctgtgg agccttcggg cttcacaggc tttgttcgcc tttgtttcgt
     6181 ttattaacaa tgcgaagtgg aagttattaa aatgccaaaa attaagaccg tacgcggtgc
     6241 tgctaagcgc ttcaaaaaaa ccggtaaagg tggttttaag cacaagcacg ctaacctgcg
     6301 tcacattctg accaaaaaag cgaccaaacg taaacgtcac ctgcgtccga aagccatggt
     6361 ttccaaaggc gatctgggcc tggtaatcgc gtgcctgccg tacgcataag ccgttaacgt
     6421 ttttaacttt ttaattagaa tatagataca ggagagcaca tatggctcgc gtaaaacgtg
     6481 gtgttattgc acgtgcacgt cacaagaaaa ttttgaaaca agctaaaggc tactacggtg
     6541 cgcgttctcg cgtataccgc gttgccttcc aggctgttat caaagctggt cagtatgctt
     6601 accgtgaccg tcgtcaacgt aagcgtcagt tccgtcaact gtggattgcg cgtatcaacg
     6661 cagcagcacg tcagaacggt atttcttaca gcaaattcat caatggcctg aaaaaagcct
     6721 ctgttgaaat cgaccgtaag atcctggctg atatcgcagt attcgacaaa gtagcgttca
     6781 ccgctctggt tgaaaaagcg aaagcagctc tggcataagc cagttgaaag agggagctag
     6841 tctccctctt ttcgtttcaa cgccatcaaa acattgactt ttatcgccgt agccttttca
     6901 ataaaggtct tttgaagagt accaaaaggt aacgcaagca atgaatgctg ctattttccg
     6961 cttctttttt tactttagca cctgaatcca ggaggctagc gcgtgagaag agaaacggaa
     7021 aacagcgcct gaaagcctcc cagtggaggc tttttttgta tgcgcgtttt gaaaaataaa
     7081 aagcctgtct catcaggtgt aattgtcgca gccagactgg acacggccag cgcggagaaa
     7141 ccggagcgta tttaagtacg tgagaatttc gagcacagcc cgggaccaaa atggcaagta
     7201 aaatagcctg atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga
     7261 ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg
     7321 cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact
     7381 taacccttca gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg
     7441 cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac
     7501 tggaaagcgc tgcactgaat gcgcgtctgg cggcggaaac gattgatgtc tctctgccag
     7561 gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa
     7621 gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc
     7681 ataacttcga tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt
     7741 tctggtttga cactacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca
     7801 tgaaagccca gcagccaccg attcgtatca tcgcgcctgg ccgtgtttat cgtaacgact
     7861 acgaccagac tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca
     7921 tcagctttac caacctgaaa ggcacgctgc acgacttcct gcgtaacttc tttgaggaag
     7981 atttgcagat tcgcttccgt ccttcctact tcccgtttac cgaaccttct gcagaagtgg
     8041 acgtcatggg taaaaacggt aaatggctgg aagtgctggg ctgcgggatg gtgcatccga
     8101 acgtgttgcg taacgttggc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg
     8161 ggatggagcg tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa
     8221 acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga
     8281 actgtggtta cgcgaatggg tgaacccggc gattgatagc gatgcgctgg caaatcaaat
     8341 cactatggcg ggcctggaag ttgacggtgt agaaccggtt gccggcagct tccacggcgt
     8401 ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac
     8461 aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc tgcggtgcgc caaactgccg
     8521 tcagggcctg cgtgtggcgg tagcgaccat tggtgctgtt ctgccgggtg atttcaaaat
     8581 taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg ctgtgctcct tctctgaact
     8641 gggcatttct gacgatcaca gcggcattat cgaactgcct gcggatgcgc cgattggcac
     8701 cgatatccgt gaatacctga aacttgatga caacaccatc gaaatcagcg tgacgccaaa
     8761 ccgtgccgac tgcttaggca tcattggtgt tgcgcgtgac gttgccgtgc tgaaccagct
     8821 gccgctggtt caaccggaaa tcgttccggt tggtgcgacc atcgacgaca cgctgccgat
     8881 tacagtcgaa gcgccggaag cctgcccgcg ttatcttggc cgtgtggtaa aaggcattaa
     8941 cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc
     9001 tatcgatgca gttgttgacg tcaccaacta tgtgctgctc gaactgggcc agccgatgca
     9061 cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg cggatggcga aagagggcga
     9121 aacgctggtg ctgctcgacg gtactgaagc gaagctgaat gctgacactc tggtcatcgc
     9181 cgaccacaac aaggcgctgg cgatgggcgg catcttcggt ggcgaacact ctggcgtgaa
     9241 tgacgaaaca caaaacgtgc tgctggaatg cgcgttcttt agcccgctgt ctatcaccgg
     9301 tcgtgctcgt cgtcatggcc tgcataccga tgcgtctcac cgttatgagc gtggcgttga
     9361 tccggcactg cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg
     9421 tggtgaggct ggcccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac
     9481 catcactcta cgtcgtagca aactggatcg cctgatcggc catcatattg cggatgagca
     9541 ggtaactgac attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgaatggca
     9601 ggcagttgcg ccgagctggc gtttcgatat ggagattgaa gaagatctgg ttgaagaagt
     9661 cgcgcgtgtt tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat
     9721 gggtactcac cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa
     9781 aggctatcag gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca
     9841 tccaggcgtt gaagccttac tgctgccaag cccgatctct gttgaaatgt cagcaatgcg
     9901 tctttctctg tggactggcc tactggcaac cgtggtgtac aaccagaacc gtcagcagaa
     9961 ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg
    10021 cattcgtcag gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg
    10081 gaacctggca aaagagaccg ttgatttcta tgatttgaaa ggcgatcttg aatccgttct
    10141 cgacctgacc ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca
    10201 tccggggcaa tccgcagcga tttatctgaa aggtgaacgt attggttttg ttggggttgt
    10261 tcatcctgaa ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga
    10321 gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc
    10381 gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc
    10441 cgaatgtaag aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg
    10501 cggtaagggt gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac
    10561 cagccgtaca ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt
    10621 aaaagagcga ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa
    10681 tgtcagaata tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg
    10741 aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg
    10801 gttttggtaa cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg
    10861 gcgaggatat tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa
    10921 aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg
    10981 ctctgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac
    11041 ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc
    11101 tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt
    11161 gaacaatgga tctcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa
    11221 attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc
    11281 gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct
    11341 aacggcgcag gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac
    11401 tgggcgctag ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt
    11461 ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt
    11521 atctgtagcg cactaatgac gtgggctatc tacttttcca cctcagttga tttgcgtcag
    11581 ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg
    11641 ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta
    11701 gcacttggcg agatctcggc gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg
    11761 ctggtggcag cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc
    11821 tttattggtc tggtgatccc acatattctc cggctgtgtg gtttaaccga tcatcgcgta
    11881 ttacttcccg gctgcgcgct ggcaggggcg agcgcattgc tgctggccga tattgtagcg
    11941 cgcctggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg
    12001 ccggtgttta tctggttatt gttaaaagca agacgttagc cgcaaaaaga cggtctatga
    12061 ttaaaagcta agatttttac tgaccacacc caggagaaac gatgcaagat tccattctga
    12121 cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg
    12181 tgttgttgat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg
    12241 agaatattca gaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc
    12301 agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat
    12361 ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa tggcgaagga cgccatccgc
    12421 tgtatcaaaa attgattgcc gcagcgccga ccgcagtcgc gccggaagag agcggattct
    12481 atgcccgtat ggtcagcaaa ggccgtgcac cgctgtaccc ggatgatatt ttatggaatt
    12541 ttgaaaaatt cctggttggc agggacggaa aagtcatcca gcgtttttcc ccggatatga
    12601 cgccggaaga tcccattgtg atggaaagca ttaaactggc attggcaaaa taatgtctat
    12661 tgtgatgcag ttacaagatg ttgcggaatc tacccgcctg gggccgcttt ctggcgaggt
    12721 tcgggctggg gagatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttact
    12781 ggcgcgaatg gccggaatga ccagcggtaa gggaagcatt cagttcgcgg ggcaaccact
    12841 ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac
    12901 gccgccgttt gcaatgccgg tctggcacta cctgacactg catcagcacg ataaaacgcg
    12961 taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggacgtag
    13021 caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgttgca
    13081 aatcacacca caagccaatc ccgcaggcca attgctgctt cttgatgagc cgatgaacag
    13141 tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgcgctgt gtcagcaagg
    13201 actggcgatt gtgatgagca gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc
    13261 gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agggaagagg tgctcacgcc
    13321 gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag
    13381 aatgctgatt tcgaccatct gatttcatta gtatcttgaa aatcgggtaa ttacgacgct
    13441 aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg
    13501 atcacagcac tgcttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga
    13561 ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc
    13621 acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt
    13681 gtgacgatgc gcgatcgttt cgatttgcag ctgccccgtg aaaccaaaca acaagcctct
    13741 attggcacgc aaattgataa agacgagttg ctgccaggtg acctggtctt tttcaaaacg
    13801 gggtccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac
    13861 gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat
    13921 ttctggcagg cgagacgaat ctagtatgtc gaaaaatgga cggcgatgat acatcgccgt
    13981 ccatttactc ctgaatacag tagtattttg cagacaaaaa ttccgtaaat ggattattcg
    14041 tttataatca ggataatcta aaaaaaggac ccctgaaaaa gtggttaaca tggaacaaat
    14101 gaaagttact ccttctgtaa ttgttatgtc ttatggcctc atccctttta gccggatact
    14161 gaaaaacatc cttcgagagg gacggttacc atgaagattt ttttggagaa tctttatcat
    14221 tctgattgct atttcctgcc gataagagac aaccagcaag tccttgtcgg cgttgagtta
    14281 attacccatt tctccagtga agatggcacg gtgcgtatcc cgacgagtcg ggttatagcg
    14341 caacttaccg aggagcaaca ttggcaatta ttttcagagc agttggaatt actaaaatca
    14401 tgccaacatt ttttattcaa cataaactct ttgcctggct taatttaacg ccacaagtgg
    14461 ctacattatt attagagagg gataattacg caggcgaact attaaaatat ccgtttattg
    14521 aactgttgat taatgagaat tacccgcatc tcaatgaagg aaaagataat cgcggtcttt
    14581 tatccttatc gcaagtgtat ccccttgtgt taggtaattt gggagcaggc aatagcacaa
    14641 tgaaagcggt ttttgatggt cttttcaccc gcgtcatgct ggataaaagt tttattcagc
    14701 aacaaatcac gcaccgctcc ttcgaaccgt ttattcgtgc gatccaggcg caaatttccc
    14761 cttgttgcaa ttgcatcatt gcgggaggca ttgatacggc agaaatattg gcacagatta
    14821 cccccttcga tttccatgct ctccagggat gcctgtggcc tgccgttccc ataaatcaga
    14881 taacgacgtt ggttcagcta taacccttct gtttgctggt gtttaagacg agagtaaccg
    14941 tctacactat caaacaggag gatctatgac cctgtctttt gttacccgct ggcgcgatga
    15001 attgccagaa acctatacag cactttcccc tacgccttta aataatgccc ggctgatttg
    15061 gcataatacc gaactggcta acacgctgag tattccatcg tcgctgttta aaaatggcgc
    15121 aggtgtctgg ggcggcgaag ccctactgcc tggtatgtca ccactggcgc aggtttacag
    15181 tggtcatcag ttcggcgtct gggcgggcca actgggtgat gggcgcggca ttttactcgg
    15241 cgaacaactg cttgctgatg gcactacaat ggactggcat ctgaaaggtg ctggcctgac
    15301 gccttattcg cgaatgggtg atggacgggc ggttttacgt tcgacgatac gagaaagtct
    15361 cgccagtgag gcgatgcatt atctgggcat tccgacgacc cgcgcgttaa gtatcgtcac
    15421 cagcgattcg ccagtgtatc gggaaacggc ggagccaggc gcgatgctga tgcgtgtggc
    15481 accaagtcat ctgcgctttg gtcatttcga acatttttac taccgccgcg agtcggaaaa
    15541 ggttcgtcag ttagctgact ttgctattcg tcattactgg tcacatcttg aagatgatga
    15601 ggacaaatac cgtctctggt ttagcgatgt tgtcgcacgt accgcgtcgt taattgccca
    15661 atggcagacg gtcggctttg ctcatggggt gatgaatacc gacaacatgt cgctgctggg
    15721 gctgacgctt gattacgggc cgtttggttt tcttgatgat tacgaacccg gttttatttg
    15781 taatcactcg gatcatcaag ggcgttacag ctttgataat caacctgccg tcgcgttgtg
    15841 gaatttacag cgtctggcgc agacattgtc gccatttgtt gccgtagatg ccctgaatga
    15901 ggccctggac agctatcagc aggttttgtt gacgcattat ggagaacgga tgcggcagaa
    15961 actgggcttc atgacggagc aaaaagagga taacgcgcta ctgaatgaat tattcagtct
    16021 gatggcgcga gagcgcagcg attatacccg cacattccgc atgctgagtc tgaccgagca
    16081 gcacagcgcg gcgtcaccgc tacgtgatga gtttattgat cgtgcggcat ttgatgactg
    16141 gtttgcccgt tatcgggggc gtttgcaaca agacgaggtt agcgatagtg agcgtcagca
    16201 gctgatgcaa agcgttaacc cagctctggt gttgcgcaac tggttggcgc aacgggcgat
    16261 tgaagcggca gaaaagggtg atatgacgga attgcaccgc ctgcatgagg cgttgcgaaa
    16321 tcctttcagc gacagagctg atgactatgt cagtcgtcca cctgactggg gtaaacggct
    16381 ggaagtcagc tgctcgagtt aaaggctact tggtcaacag cagcttgcca gcctgagttt
    16441 tacggagcag atattcttgc ccgtcatgat cgataatcag tttgccatcc ggacctaaca
    16501 gtgtctggct ggaaattcgc cgaataggct gcggggaagc ggtcttgtga ttggcatccg
    16561 tttcaggcgt gagttttctg ctatccgtat aacgcataaa aattaataat aattgatata
    16621 acaatgattc ttattatcaa tattatggct gaccagagca agcagtaatg tgctgagaag
    16681 acaacaagtt gcgcagaaat gcccatccca tcaggaatgg gcacgcattc agaagcgggt
    16741 atctaccgca gaggcgagtt tttcgaccag gcgttcggta tcttcccagc ccagacacgg
    16801 gtcggtaatg gattgaccgt aagtgagcgg ctgaccgccg acgatttttt gcgttccttc
    16861 gcgcaggaaa ctttccgcca taattccagc aatcgccgta gagccattgc ggatttgctg
    16921 acaaatatcc tcacaaactt ctaactggcg acggtgctgc ttctggcagt taccgtggct
    16981 gaaatccacc accagatgtt caggtaaatc aaactcgtgc agcgtatcgc aggctgcggc
    17041 gatatcatcg gcatgataat tcggtttttt gccgccacgc ataataatgt ggccatacgg
    17101 gttgccgctg gtctgataga tggtcatctg accatttttg tctggcgaga ggaacatatg
    17161 gctggcgcgg gctgcgcgga tagcatccac agcaatccgc gtgttgccat cggtaccatt
    17221 tttaaaacct accggacagg agagtgccga agccatttca cggtggatct gactttcggt
    17281 agtacgtgcg ccaatcgcgc cccaactgat taaatcagca ataaactgac cggtcaccat
    17341 atcgaggaac tcagtcgcgg ttggaacgcc tagctcattt acctgtaaaa gtaatttgcg
    17401 cgccagctcc agaccgtgat ttacccgata gctaccgttt aaatccggat cggagattag
    17461 tcctttccag ccgacaacag ttcgtggttt ttcaaaatag gtgcgcatta cgatttccag
    17521 ccgtgactgg tactggttgc gcagcgactg cagacgggtg gcgtactcca ttgcagcggt
    17581 aagatcgtgg atcgagcagg ggccaataat gaccaacagt cgcttatctt caccattgag
    17641 tattttttca attctgcggc gggagtcggt gacatgggtg gcgacgccag gcgttacggg
    17701 ataccgtagc gcgagttcgg cgggcgttac caggctctca atacgcgcag tacggagttc
    17761 gtcagttctg ttcattacaa gtctcagaaa tttgttgctt ctacggcagg actgccggga
    17821 agtgatgcgc atcacgataa accaatcgct gctgatttca agtgcgaggt gtgtcacacc
    17881 tcgcagggtg gttagcatga taacaaaaaa ataagctaat gcactagttc tctagtacat
    17941 tcggcgacta aggcccatga tatcgaggat cttggtggca atctcttcta ccgaataatt
    18001 ggtactgtta atccacggga tctgattttt acggtacaag gcttccactt ccgcgacttc
    18061 catcctgcac tgacgaagcg aggcatagcg actgttctcc cgacgttcct cgcgaatcgc
    18121 cgccagacgt tccgggtcga tagtcaggcc gaacaattta tgctgaagcg gtttgagcga
    18181 cgcgggtagc accagattat ccatatcgtc ggcaataaag gggtagtttg ccgcgcggat
    18241 accaaattgc attgccagat acagactggt gggggtttta ccacagcgag aaacaccgag
    18301 caggatcacc tgagcctgat ccagattgcg caacgaaatg ccgtcatcgt gggcgagggt
    18361 gtaatcaatc gccgcaatgc gcgcatcata tttattgaga ttattagggt taaggccatg
    18421 ggtacgatga gcaatcggcg ttggatccag tttcatctct tgttgtagcg gggcaaccag
    18481 cgcctgaacg atatcctggc aaaaaccttc actttgcaag atgatggcgc gaatctccgg
    18541 caacacgatg gagtagaaga ccagcgggcg cacgcctgtc tggtgataaa ttgcgtcaat
    18601 ctgatccttc actgcccgtg cacggctctc attttcgaca aacggcagcg tgatgctgct
    18661 gatagtgacg ggaaattgtg acattactgc gtgtcctaat acctccgcag ttattgccgt
    18721 accatcagaa atataaaaaa cgtggcgatc aacagcatta tccattttgt tcttcccgtg
    18781 atgcagacat aattctatga aagcataaat taaaaacgca cagaagcgta gaacgttatg
    18841 tctggtttat aaaatgaacc ttcaatttta ttttttatga aaacagcatt tcatttttat
    18901 ggtttcgttt ataccgatgg tttatgtgga aattgtcgaa gagagcagat ttgcgcaacg
    18961 ctgggatcag tcttaaaaag taaaaaaata tatttgcttg aacgattcac cgtttttttc
    19021 atccggttaa atatgcaaag ataaatgcgc agaaatgtgt ttctcaaacc gttcatttat
    19081 cacaaaagga ttgttcgatg tccaacaatg gctcgtcacc gctggtgctt tggtataacc
    19141 aactcggcat gaatgatgta gacagggttg ggggcaaaaa tgcctccctg ggtgaaatga
    19201 ttactaacct ttccggaatg ggtgtttccg ttccgaatgg tttcgccaca accgccgacg
    19261 cgtttaacca gtttctggac caaagcggcg taaaccagcg catttatgaa ctgctggata
    19321 aaacggatat tgacgatgtt actcagcttg cgaaagcggg cgcgcaaatc cgccagtgga
    19381 ttatcgacac tcccttccag cctgagctgg aaaacgccat ccgcgaagcc tatgcacagc
    19441 tttccgccga tgacgaaaac gcctcttttg cggtgcgctc ctccgccacc gcagaagata
    19501 tgccggacgc ttcttttgcc ggtcagcagg aaaccttcct caacgttcag ggttttgacg
    19561 ccgttctcgt ggcagtgaaa catgtatttg cttctctgtt taacgatcgc gccatctctt
    19621 atcgtgtgca ccagggttac gatcaccgtg gtgtggcgct ctccgccggt gttcaacgga
    19681 tggtgcgctc tgatctcgca tcatctggcg tgatgttctc cattgatacc gaatccggct
    19741 ttgaccaggt ggtgtttatc acttccgcat ggggccttgg tgagatggtc gtgcagggtg
    19801 cggttaaccc ggatgagttt tacgtgcata aaccgacact ggcggcgaat cgcccggcta
    19861 tcgtgcgccg caccatgggg tcgaaaaaaa tccgcatggt ttacgcgccg acccaggagc
    19921 acggcaagca ggttaaaatc gaagacgtac cgcaggaaca gcgtgacatc ttctcgctga
    19981 ccaacgaaga agtgcaggaa ctggcaaaac aggccgtaca aattgagaaa cactacggtc
    20041 gcccgatgga tattgagtgg gcgaaagatg gccacaccgg caaactgttc attgtgcagg
    20101 cgcgtccgga aaccgtgcgc tcacgcggtc aggtcatgga gcgttatacg ctgcattcac
    20161 agggtaagat tatcgccgaa ggccgtgcta tcggtcatcg catcggtgcg ggtccggtga
    20221 aagtcatcca tgacatcagc gaaatgaacc gcatcgaacc tggtgacgtg ctggttactg
    20281 acatgaccga cccggactgg gaaccgatca tgaagaaagc atctgccatt gtcaccaacc
    20341 gtggcggtcg tacctgtcac gcggcgatca tcgctcgtga actgggcatt ccggcggtag
    20401 tgggctgtgg agatgcaaca gaacggatga aagacggtga gaacgtcact gtttcttgtg
    20461 ccgaaggtga taccggttac gtctatgcgg agttgctgga atttagcgtg aaaagctcca
    20521 gcgtagaaac gatgccggat ctgccgttga aagtaatgat gaacgtcggt aacccggacc
    20581 gagctttcga cttcgcctgt ctgccgaacg aaggcgtggg acttgcgcgt ctggaattta
    20641 tcatcaaccg tatgattggc gtccacccac gcgcactgct tgagtttgac gatcaggaac
    20701 cgcagttgca aaacgaaatc cgcgagatga tgaaaggttt tgattctccg cgtgaatttt
    20761 acgttggtcg tctgactgaa gggatcgcga cgctgggtgc cgcgttttat ccgaagcgcg
    20821 tcattgtccg tctctctgat tttaaatcga acgaatatgc caacctggtc ggtggtgagc
    20881 gttatgagcc agatgaagag aacccgatgc tcggcttccg tggcgcggga cgctatgttt
    20941 ccgacagctt ccgcgactgt ttcgcgctgg agtgtgaagc agtgaaacgt gtgcgcaacg
    21001 acatggggct gaccaacgtt gagatcatga tcccgttcgt gcgtaccgta gatcaggcga
    21061 aagcggtggt tgaagaactg gcgcgtcagg ggctgaaacg tggcgagaac gggctgaaaa
    21121 tcatcatgat gtgtgaaatc ccgtccaacg ccttgctggc agagcagttc ctcgaatatt
    21181 tcgacggctt ctcaattggc tcaaacgaca tgacgcagct ggcgctcggt ttggaccgtg
    21241 actccggcgt ggtgtctgaa ttgttcgatg agcgcaacga tgcggtgaaa gcactgctgt
    21301 cgatggctat ccgtgccgcg aagaaacagg gcaaatatgt cgggatttgc ggtcagggtc
    21361 cgtccgacca cgaagacttt gccgcatggt tgatggaaga ggggatcgat agcctgtctc
    21421 tgaacccgga caccgtggtg caaacctggt taagcctggc tgaactgaag aaataaaata
    21481 aatacccggc ggcgttcagt cgccggggtt atgtgatccc cgaagatgaa acttattcaa
    21541 tctcttcaca gacatcctgc gttaatcgcc gcataatatc ttttcttaac aaaaactttt
    21601 gtattttacc tgaggcagta cgcggtagtt tttcgattac cacgatatgt tcagggtatt
    21661 tatattttgc gacccgttta cggctaaaaa aagccaccac ctcttccagc gataaggaat
    21721 gatgcggcgc tttcagcacg acataagcgc atgatcgttc acctaaacgt tcatcgggca
    21781 ttgcaaccac acaggcatcg tgaattttag gatgctgcaa taaaatatct tccacttcac
    21841 ggctgctaat attttcgccg ccgcggacaa taatatcttt tttgcgtccg gtaattttta
    21901 tatagccagc ctcatccatg cggcagagat cgccgctgta ataccagcct tcttcatcca
    21961 gcgcacgggc ggttaattca ggttcatcaa aataccccat aaacacattg gggccacgcg
    22021 aggcttcttc accttcacaa cctggcggta aggtcttgcg tgcgtcatcg accactttaa
    22081 tctctacacc tgcggcagcg taaccatcgg tgtgcataaa gcgcgacaaa ggatcgtcga
    22141 gattcaccac cgcatgcggc gaactttctg tggaaccata aacacttaat aatttaatgc
    22201 cgcgctgctg gcattcacgt gccacttttt tggggattgt ggtaccgccg caaagaaaga
    22261 aacgcagcgc tgaaaggtct gcgggctgtt tctctagtaa attcaaaaga tcatagacaa
    22321 acggcgttgc gccgagcata caggtgcaac gctgctgctc aagcagcgcg agacacgcat
    22381 caggagtgaa aatatctaac aacacgctgc gagcgccaat taagaatggt gccgttacgc
    22441 catgcagaaa gcccgttgcg tgaccgagtg gcgcaggcat cataaagaca tcctgccagg
    22501 tcagattcag ccgcgcgcaa taagcccgct cactggcgag aatattgtta tgcgtaagca
    22561 tcacgccctt tggcaggccc tcggttccgg aggtaaacag caccgcggct aattcatcgc
    22621 cgtgggtcgt tatcgccgtg gtcagtgggg tattgtcggc gataatctga cttaatgaga
    22681 gggaagaggt ggcgggagcc agtttgtcca cgccgacaat ttgttgtagt tgtggaagct
    22741 gattttgcag cggcaggatt aaatctaccg gacgcgtttg tttaaacaac gtcggtgcaa
    22801 agaacatttt tgcctgacac ttattgagca cccataccag ttctgcttcc cgccaggaag
    22861 gcaacagcgg tacggaaacc gcaccgattt tcaggcaggc aagatagata acggtaaatt
    22921 cacaccagcc tggcagttga aatgcgatgc gatcgcctga ttcaataccc ttcgctaaca
    22981 tccagtttgc cagacagctc gcggcgtgat cgagcgcgct ataggtgtac gatgcaccat
    23041 gattatcgac cacggcaatt ttgtctggca tcgcacgagc ggtctgctgc cagtaatcgg
    23101 ccagcgaagc atcgccccat agcccttgct gacgatacgc cgcacgacgt tgttcgttga
    23161 acgttaatgt cactttcatg ttggactctc gaaacagaac atcaggcccg agatgcgggc
    23221 ctgtgggatg catgattagc cgtagcgaaa atcaataccg aaggtgcctg cgggatattg
    23281 ccactgttcg agaatagtgt taccgcacag cacccgacag gtgccgcact ccagacaacc
    23341 ggcggaatca aaatgaatgt ttcctgcgtc atcctgctta taaagtccgg cagggcaggc
    23401 tttcattaat ttatggaatt cattgatatc gggattttcc gccagaatga tatgcgggtg
    23461 gccctcatca acatggaatt tattgacgcc taatttgatg tcgacgttaa ccgtagcgtt
    23521 ctggctcata gcgcggttgc tcccttaatg ccatctttca gcaagttgat cagcccaatt
    23581 ttcttcgcgt gtcccatgat cattttgcgt accggctggt ttggtttgcc gtcgatggtg
    23641 aacatctcgt tcatgatgtc ggctaccatt cgtgggtatt ggctaaacag gcgcgggttt
    23701 tccatcagcg ccgggatctt gcgaaaatgc tgcatatcac gcatgacgca gctttgttcc
    23761 agctcacgtt tgtattgcgc cagactgctg gcggagaaat cctcgcgttc tttggcggcg
    23821 atcaccgttg tggcggcagc ctgagccgat gcaatggcta aatccatgcc acgaactgta
    23881 aaacccaaat tcaggcagaa gcctgcggcg tcaccaacga tcatcacgcc atcgttaacc
    23941 agctgcggca ccattgccag accgccttct ggcaccatat gcgcggaata ttcaagcaac
    24001 ttgccgccgc taatcagcgg gcgaatggcg gggtgttgtt taaaatcttc cagcatttgc
    24061 ggcacgcttt tttgcgcatg ggcgatatca cccaatccac aaaccagccc caaggatatg
    24121 gaatccttgt tggtatagag aaatcccccg cccatcaggc cgtcagaagg ggaaccggca
    24181 aacagccagg cggcaccttc ctcgcccgta atattaaagc gatcgttgat ctgttctggt
    24241 gtcaggccaa taacctcttt aacaccaaca gcgtaatgat gcggatcgga agcgggaacc
    24301 atttccagcg agcggccaag catcgagtta acgccatcag ccagaatcac cacattcgct
    24361 tcgagaatat catccccagc ctgcacgcca gtgaccttgt ttccttcacg aaccagcgca
    24421 tcgacgcgta cgcccgggat aaactgcgcg cccgcctgct cggcttgttc catcaaccac
    24481 gggtccatac gattacgcaa tacggtataa gatgcgtgtt gcggaacatc tggttgctcg
    24541 cggtgaaaat cgagggtaac ggcgctttct tcggttaaga aggagatttt ctcgcgtgtg
    24601 accttgcgtt ctaccggcgc tgatactgca aagcctggaa tgattgcttc aagtgtgtgg
    24661 gcataaagac gcccgccggt catgttttta catccggcac tgtcgccgcg ttctatcacc
    24721 aggacatcca gcccggctcg tgccatgacc agtgcggcaa cgctaccagc aacgcccgca
    24781 ccgaccacaa tggcatcaaa tttgtcatcc gacatgcgtt atgctccctt cccgctgcgt
    24841 ttacagcggg agatgtggtt gaaaggggaa ttagcggctc aactggctaa tcagggcagg
    24901 gacgactttg tagatatcgc ccaccagacc gtagtcggca tagttgaaga ttggcgcatt
    24961 tttatcttta ttgatggcga caatcacttt tgcgccgttg ccgccaacca tatgctggat
    25021 ctgcccggag atccccagcg tcaggtagag atcggatttc agcaacacgc cggagacacc
    25081 gatataacgt tcacgctcca tccagttctc gccttcggca attggacgtg aacagccgac
    25141 ttcagcattc agcaccgccg ccagttcgtg gaccattttc aggtcatcct gcgccgccag
    25201 tccacgaccg acgccaacca cacgttttgc tttgctcagg tccacgctgc ttgcggcttt
    25261 cgcacggcgt tcgcgacaga gaatttcatg acgcggagca acataaggta ccgtttctgt
    25321 cgggcactga tgagaggtat cactggtgca cggttcctga acaccgggtg caagggtaat
    25381 gatcgccagc gggctgttga tcttttcctg agcgaacgct aacccgccat acatccggtg
    25441 ttcggcgcaa atgtgaccat cgacaatatc caccgccgtg gcatcgttca ccagcgccgc
    25501 attcagttgc acacttaacc gtgctgccag cgctttacca cgtttcgtcg cggccaacag
    25561 caacatagcg gggtgtttat ctttcagcag ggcagcaatg ctttcggcgt aattttcagt
    25621 gcgttgcagc gcgtcgtttt gcgcaagaac ataaatacat tttggaccat aaggcataac
    25681 tgcctgcgcc tggtcggtat tttgtacaat ggcatacacc tgttggcccc attgctgagc
    25741 gccgccaaac agttcagcat aacgttcagg attatcgcta aagacccaga cgctgtttaa
    25801 ttgactcatc gtttcttctc cgtaggcttc agttcagggc tttcttcaga tgttccgcca
    25861 gctcggcaat ggcctccggc gaatcgttat cgagaatgat gtgcttacgt tctgtttgcg
    25921 gcggtacgcg aatgccgacc agttcagcaa gtggcgcgct ctggctccag ccaatatcac
    25981 ttgcctgcca ctgatttacc ggttttttac ccgcgccaag aatggctttc atcgaaggaa
    26041 tgcgtggcac gttaatatcg gaggtgacgc agagcacggc tggaacagag agttcaataa
    26101 cttcaacatc atcttcaagc gtgcgttcaa tcaccagtgt attgccctga cgctgaatag
    26161 cactcactgc attaatcacc ggaagttgca gaatttctcc gaccagcaag ccaacctgct
    26221 gggcataaag gtcgccggaa ccttcaccaa agatcagtaa atcgaagccg atcttttcaa
    26281 ttgctgccgc cagcgctttt gcggtatcga gaggcagtgc atgttcaagt tgcgcatcct
    26341 gcaccaaata caggctgtgc ggcccgcggg atagcacgtc tttgcgcact ttcgagttct
    26401 gcaacaatga gccgccaacg gtcagcgcgg ctatctcatc gtcatctgtt gcgagctggc
    26461 ttgcagcttc aatggcattg agatcgaact ggctgatttt ggcgtcggca ttgtcgaaat
    26521 tcagggtgta ttctggagtg acaacaatgt cctgttcttc aggcaccagc ttaaagcagg
    26581 ttattatttt catgatttct cctgtgagtc gcatacggaa ccatctccga tggaaggtaa
    26641 aaatggcctg aaaatagtgt gcgatagcgg tgcggggttt tcgacggagc aagctgtttt
    26701 ccttgccaac aatcttgcaa aaaaaatgcg atcgctaaaa caaaagtgca atttgcggca
    26761 atatagttat tccatgaccg tacaatcttg cattcagtgt aaataatctt gtttagctct
    26821 cgctaaacgc ggttgtataa ccgcaataca cgccgtattt tatccggcat atattgtttt
    26881 tgcttgctca tggaataggg aggttgtcat gtatcaacgc tgttttgata acgccagtga
    26941 aacgctgttt gtcgccggta aaacgccacg gctttcacgt tttgcattta gcgatgatcc
    27001 aaaatgggag tccggacatc acgtgcatga caatgaaacc gagttgattt acgtcaagaa
    27061 aggggttgca aggtttacca tcgattcttc gttatatgtc gcacatgcgg atgacattgt
    27121 ggtgatagaa cgcggcaggc tgcatgcggt ggcctctgac gttaacgatc cggcaacgac
    27181 gtgtacctgt gcgctgtacg gctttcagtt tcagggggct gaggaaaatc atctactgca
    27241 accgcattct tgtccggtaa ttgccgcggg gcagggaaaa gaagtcatta aaaccttatt
    27301 taatgagcta agtgtgattt tgccgcaaag taaaaatagc caaacatctt cgttatggga
    27361 tgcatttgcc tatacattag caattcttta ctacgaaaac tttaaaaatg cttatcgttc
    27421 ggagcagggg tatattaaaa aagatgttct gataaaagat attcttttct atctgaataa
    27481 taattattgc gaaaaaatca ctttagaaca gttatcgaaa aaatttcgtg ccagcgtcag
    27541 ttatatttgc catgaattta ccaaagagta tcgtatttcc cctattaact atgttattca
    27601 acggcgtatg acggaagcaa aatggtcact cactaatact gaattatcac aggcagagat
    27661 ctcctggcgc gtgggttatg aaaatgtcga tcactttgcc aaactgtttt tgcgccatgt
    27721 cggttgttcg cccagcgatt accgtaggca atttaaaaat tgttttgcgg aacaagaaat
    27781 cctatctgta tttcctcaac cggtaagtct tgttggataa atctggcggc gcagtttgtt
    27841 aagaagagct gcgccgcctg cagattattt gttctgatag tctttcagga tctgccgacc
    27901 tgctacgtaa atcataattt cgtctgtacc gccgccgata cgttcacaac ggacatcacg
    27961 ccagaagcga gagacgcgcg cctcatcggt atagcccaga ccgcccatga tttgaatcgc
    28021 atcatcaatg acttccattg cggtacgtgc acagtacagt tttgccagcg ccgcgctggt
    28081 gcgcagtgat tgatgctgat cggcttgcca tgccactttc agcaccatgt tgcgcatgtt
    28141 gtcgatctta atcgccatca gcgccagttt ttcctggatc atctggttat gcccaatggg
    28201 tttaccaaaa gcgatacgct ggttggcata gcgagcggca tcttcaaagg cacactcggc
    28261 aaagccggtg ctgcgcgcgg cgttgatcag gcgctccatc tcaaagttgt acatcacatt
    28321 gaggaaaccc attccttctt cgcccaccat gtcgctctct tcaacttcca cgttgtcgag
    28381 atagacttcg caggtgctga gcatatgcca gccgatttta tgcagcgggt taatcttaat
    28441 gccgggctta ctggagtcga cccaccacag ggtgaaggct tttttgggat ctttcggttg
    28501 cggatcgcgc gccaacacca gcatatacgg gtactctttc gcaccggtaa taaaggtttt
    28561 ctgtccgttg atgtaaacct tgccgttttt acgcgtgtaa gtggtagtgg cactgttgtt
    28621 gtctgagcct gcgcctggct ccgtcaacgc cagggcatag gcgggatcac ccgtttccag
    28681 tgtgctttct gccgttttac gtagctgctc tgcagaaccg aaacggcgca tactgtgaat
    28741 acattgaccg ttggtaatca aaaatgccgg agcaccgcat tttgacactt ccatcagcgc
    28801 cagcatctgg gtaacgtaat ccgcagggat gccgccgaac tcttccggca caccaagcat
    28861 ggaaataccg ttatccgcca gtgcccgcat aaactcacgc gggtatgtcc cgttttgatc
    28921 gcaggtgcgg aaatactctt ccggaaagtt agtcgtaatc aactcgcgaa tactggccag
    28981 cagcagttct tgttcttcag ttaaagaaaa atccatcgtg atactcctgt taatgagccg
    29041 cttcaggcag gacaaaaccc atcgccgcat cgataaataa tctttcgtcc atcagtttga
    29101 gttctggcga aatcactggg gtgaaatcca ttttgtcgag aatatctttt tgtaaatcga
    29161 cgccaggagc gatttcgatt aaatgcaggc cgtcttcttt cagcgtgaat acagcacgtt
    29221 cggtgatgta acgaacatcc agtccccgtt cgagcgcgat ttttccgctg aaagtaattt
    29281 ccggtagttc ccgaataaat ttcttcaccc gtccttcctg gacgatattt aatttgccgt
    29341 cggtaatttc tgtttttaaa ctgcccgcag ttaacgtgcc gcagaaaata attttcttcg
    29401 aggtggcact gatatcaata aatccaccgg tacccatgat tttgccattg aatttatgca
    29461 cgccgacgtt accgtgctgg tcgacttcag caaaactcaa ataacaaaca tccagaccgc
    29521 caccgtgata aaaatcaaac tgggacgtca tatccagaat ggcacgggta ttcacgttcg
    29581 cgccaaaggc gatcccctgt gaagtaattc cgccaatcgg acctgtttct accgtcagaa
    29641 taaagtcatc agcacaacct tcttcccgag cgaccaggcc aatgccgtca gcaataccga
    29701 cgccgacatt ccccaccgcg cctttacgca tttcgaataa tgcgcgccgc gcaactaatt
    29761 tacgttggtt taggggcagg ctaagtttga tactgtcatc aagggtgaag tcaccagaaa
    29821 taaagcggtt aaccggtgca ccgccataca gttggctttg atccggatcg accaccacaa
    29881 tatccaccag ataacccgga atacggacag atttaggatg cagcgtggct ttcttaacca
    29941 ttttctgcac ctgcatcatc acaataccgc cgttattgtg caccgcctgg gcaataacca
    30001 atgcgtcgag atacatcacc tcatcttcaa aagtggcgta gccttcactg tcgcaggtgg
    30061 tagcgcgaat gaaggcaata tctggcgcaa tcgctttgta atagagatat tctttgttat
    30121 caaactcgac cagtttaatc aggtcttctt tagtgacttc attcaatttg ccgccttgct
    30181 ggcgtggatc gacaaatgtc ccgatgccaa tatcactaat aataccagac tggtgggctg
    30241 cggcggcgcg taaggtttgt gtaagtacac cttgtggata gttataagca ataattttat
    30301 tttgttctgc gagttcagaa atacgcggcg attgtcccca gtgaccgcat aatgcccatt
    30361 tcaccagacc ttcttgcgcc agaggactaa taccacggtc ggcgcgatcg ccaagccctg
    30421 ttggactaat aatcgataaa ttacgtggcg tttgagtctg tttatattta tcagcaagag
    30481 cagtaattaa cgtggtggct tccagaatac cgccgccagc gcccaacaca caaagtgttg
    30541 cttcgtcggg aatataattc accgcttcct gtgccgacag gaccggcact cgtccattaa
    30601 tacgaagtgg ttttacaggt ttcattgtta attcctgatt caggtgtgat tttccctacg
    30661 ccagatggag aattagcgca cagagactca cgaaatattt ttataaaata cggttaaaaa
    30721 tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc ttatgcctga
    30781 tgtaaaatag ttaatactgt gcgcaaatca tttaccgaga tttgccctgg cgcagaggct
    30841 ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccagccag acgagaaatt
    30901 acgccagttt ttgccattga cattgtgatg attggacgat cggcatactg ctcctgcatc
    30961 tccagggtcg cggcaagcaa cgtcagcaca tcactggtac tttgcggcat cagcgcaatc
    31021 ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat ttcttcggct
    31081 tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg cgcgtgggcg
    31141 taggtgacgg tttctttaac ctgatcatcg ccggtaaata actccagatc gatcatatca
    31201 accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt ggaaatcgcc
    31261 tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtgtttc tggcatggtt
    31321 tcacggagaa tttttgctgc cgccatgaca gactccacat tggagagatc ggcatagtgg
    31381 tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc ttcggatttc
    31441 acgctggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc cgtaccaatg
    31501 acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg gtcagcctgt
    31561 caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt gccagtccat
    31621 aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat cgttttgcaa
    31681 ccagcttgtt gcgcctgctg caataacttc gtcatatgcg ggttatacac gcattcagtg
    31741 accagaagtc ccggatgtaa cagactgata tcattaacta atgattcatt ctcaaggggt
    31801 ttcataccca cttttgtgcc attggttaaa atgtcggcgg aagctagggc ttcagcaaag
    31861 gcttgctgat cggcgagatc ggtaaccgtg acgacacaat cggtgttttc gttaacccgc
    31921 tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag tttaatttct
    31981 tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc ggcccctaac
    32041 agcaccattg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg aatatggccc
    32101 gtgccgtcgg tgttatagcc gcgcaggtag ccatcatcat taacgatggt gttgatggca
    32161 cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag ttgtttgttc
    32221 ggcatcgaca caccagttcc gcgcattttt agagctttta atccttcaat tgctccggga
    32281 aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc tttttctaag
    32341 gctttattct gcatttcagg cgataaactg tggcggatag gataagccat caacccaatc
    32401 aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc ttaattgtgt
    32461 ttaaccgggt gcaaaatttg cgctcgccaa accgcacatc attctcagga ataataaaaa
    32521 cgcggtaata gcggataaaa acaataattg cggtaatcag ggccagcagc gcgaaagtaa
    32581 aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta atcagcggaa
    32641 taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg gctttgcttt
    32701 ttgggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca gctgctgaga
    32761 aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg tagataatgg
    32821 ctgctgttat tgttgccagt gcagagttaa atacattagc ccagatgggc cggaccattt
    32881 ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg taataagaga
    32941 tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat ttgggcatcc
    33001 acactacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc gatgaaacac
    33061 cttccagcca gactaacggt ttgctgttca tttgcggtaa ttcattagct acgctggcgt
    33121 ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt acaaacagaa
    33181 tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata tagctcacca
    33241 gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg accgccgaac
    33301 cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc agcgctgagt
    33361 tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg caagcgggaa
    33421 taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc cgacgaccaa
    33481 acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa ccaataccgg
    33541 aaattaagta ggcaatgccc gcgttgtcag tggaaaactt ttccgccaga gatgacatgt
    33601 tttgggcaag cgtaataaca ctaatgccgt gcaggaagta gctgaagtaa atacaaagaa
    33661 cagccaggat aaatggcgtg ctgaaagcct tattttgaga cattttcaca gctccttgct
    33721 gaaagataat ttcgttaccg tacgtattgt tctccgacgg tttacataat tactatactg
    33781 aagatgattt tccctgggta catggcataa agtttatgtt tccaggaata ttgcatgtca
    33841 tagtttgttt ttgatcgcga ttttccttag ttagcgcaga aagagttatt tatccgtaag
    33901 atcttgcatg acctgaaaag aatattgcat tacccacccg gagcgacatt ttcctttagc
    33961 gagtgatgcc gcgttttttt gcggctgcgt aagccgataa acagtgccag taaaaaaccg
    34021 atggtagcga tggcggtatc gaaccacata atatcggcaa tacttctttg ggacagatga
    34081 gccgtaatca acggaatagt aaaggtcgca atactgcccg cactgtaata gatccctgta
    34141 gctttacctt tagcgtaagg aaaacgttca gccattaacg tcaagccaat ttgcacaaca
    34201 cctccagcag aggtaaaacc aatgacaaaa gcaaatatta tcaccacata aaatgtggga
    34261 tgcaggcaga cggtaaacag agcaataaat gagataaagg tgtacagcat gagtaatgtt
    34321 gttgggcgaa cggtattacg aatgagtgga gcggtaataa atacacaaag cagcgaaccc
    34381 acagtgtaga tactgagtag tttgatcgac atagtgtatg acatgcctgc aacaaattgt
    34441 ccgtactgtg ccagccactg gctaaccaga taaaacgttg ccattgagat atagccatat
    34501 aaggtataac tggctaaatc gataattgaa cagcgatgtt ccgtagagct ggtggttttc
    34561 tttatgacag gtaagcgacg acccggatgg ggtgggaacg tacaacggta taaaaacaga
    34621 gcgttaataa acataatgcc tgcagcaatc ataaaggacc aaccgaacca cagttcagcc
    34681 cacactaaca ggctaatgat tagcggtaat aaaaattgtc cgctggaaac aaatgcttta
    34741 attaaaatat tggctgtccc aggtgagcgt ggaaaagctt ccatcaaact gggataagtg
    34801 cctgcatcga gaaaactgtt tgccattccc gccagaaagc caaaaacata agcgataatg
    34861 atgttattgg tgtgcaggat gccaaaaaag aaggccatat agcagcacat cccgagcatg
    34921 ataaaagggc ggcgaccaaa gcgatcggat aataatcctg caaaaagcaa gacacttaat
    34981 cgaccaatgc ccagcgatga gataactatc gagacgcccg cggcattagt ctgccaaagc
    35041 gtctccagcg aggccatatt caggctcatc aaaatgacgc ccataccatg caccaggtaa
    35101 ttaaaataca acccaagtgc ggtagggtaa tagggatttt tcataggttc tacttagcaa
    35161 aggtgcttta aaaagtgtag cgatgttatg tcagtatccg ttcattattg ttgtaacaac
    35221 gacaattcct ttctgtggaa aaattaacaa agccgctcca gatcgtgtgc atataattca
    35281 gcggctaccg attgatagat cttccagtca ataaaattgc catcagggta gactcgttga
    35341 aatgcggtta agtcggggaa aaaacgcatg gtgttgttgc cgatgcggtt atttattttc
    35401 tctatgatgg catgaagatg tttttttctt tgctgacgca tagccaacag ttgctcgact
    35461 acacgatcag gaatggcaca cttgccattt tcccactgac gccagctttc actgtcaccg
    35521 gtctgggcaa tccaggtcgc gcattcatca atagtcatgg caaaaatatg gcggagtgcc
    35581 tggagttcat aagcgttcat cgtgtaatgg ctgcatgaaa taaacaataa aatataaata
    35641 acattatcag cctgctgacg gcttgaagag agtcacagta tcttgtgcaa cattattgga
    35701 aagagtgagg ggcgaggtag cgaagcggaa aaatgttaaa aaaaagccca tcgtgggaga
    35761 tgggcaaaga ctacacacag caattcgttg tttcactcag gggatttcca tgcttataaa
    35821 tcaatatgtt gatttataac cgtgagctaa tagtaggcat acggactttt tgcgtcgatc
    35881 agattcgtct cattagttta aaaactgtaa agaatttgcg acaggtacta ccagctgaat
    35941 ggcggtaagc aggaaaatat ggagaagagc aagcaaatat caattagcgg tggttaacaa
    36001 tcaggcggca gagccgcctg atgagaaatt acttattcgg tttctcgatt tcgccaagtt
    36061 cttcgagaat ttcttccggt tggtccgtcg gcggcgggac ttcttccacc cacgcggcaa
    36121 acagacgcca ggaaacggct aacagaaccg gaccaataaa cagaccgatc atcccgaaag
    36181 caatcaaacc accaataacg ccagagagga tcaggatcag cggtaaatcg gcacccatgc
    36241 gaattaacat tgggcggatg acgttatcca gcgtgccaac cacaccgctc cacactaaca
    36301 ataccgttcc ccaggtggta tcgccagtcc agtagagcca gataatcgcc ggaatcagta
    36361 ccggcaacgg gccaagctgg acaaggcagg aaaggatcat taacaccgtt agcaaagttg
    36421 cataaggtac gccggatacg gcgaggccga taccgccaag cactgcctgt actaacgccg
    36481 tcaccaccac acccagcgcc accgcgcgga tagcctgtgc cgccagcagt acggcggcat
    36541 ctccgcgaac gcctgccaga cgggtcgcaa aatggcgaat gccttgtgcc acctgttcac
    36601 cgcgccagta cagcagggca ctgaagagaa gcatcagcgc acaatgcacc ataaagcgcc
    36661 cgatatgcgc cgcttgccca acgaaccagg tagtggtggt gccaatataa gggcggactt
    36721 tcgccatgat cgccgtcccc cccatatcca gcaagttgtg ccagcctgca tacagcttcg
    36781 cgccaatcac cggaatggta ttaagccacg ccagatcggg taacgtcatg tcaccgctgg
    36841 aaatggcttt aattagcggg ccgctgccgt cgacgatact gttaaccagc aaagcaatag
    36901 ggatgataaa caccatcact aataacagcg tcatcaccag aacggcgaga gagcggcggc
    36961 caaacatgat cttttgcaaa cgtaacaata ccggccaggt ggcgataacc accgtaccgg
    37021 cccatgcaaa gccgagaata aagggttgaa caatccacag acatgccaca atcatgatgg
    37081 ctaaaaacag caccgaaagc agaatttgtg cgacatccct gggctgacga acatttacca
    37141 taactacttt tcaccttttt tgtgcgtcaa atcgtcgacg cgacgtgaac acgcgaaact
    37201 cacctgcata atgatgagca atttcagcgg tttttaacag gccgattctg cccttaattc
    37261 tgatgggcgt gttagaaaaa aatgtggtac aacaactcag gcaacacgca aacgatttac
    37321 tcgtcgtatt tcaagttgca tatgctgcgg ctacgttcgt tcaccccagt tacttactgt
    37381 tgtaagctcc tggagttcac tcgcttgccg ccttcctgca actcgaaatc gatagagtaa
    37441 ataaacatca cctttctgtg cgctattgca gccgcctgaa agaacagtgt atcgacctac
    37501 atcacagacc gcaggaaagg gtcaatataa tgattccaca gatttcccag gcacccggcg
    37561 tcgttcaact ggtgcttaat tttttgcaag agctggagca acaaggtttt accggcgata
    37621 cggcgacaag ttatgccgat cgtctgacaa tgtcgaccga caacagtatt taccaacttc
    37681 tccccgatgc ggtggtattt ccgcgttcaa ccgcagatgt ggcgctgatc gcccgtcttg
    37741 ccgcgcagga acgctattca tcgctgatct ttaccccccg cggcggcggc accggcacta
    37801 acggtcaggc gctcaaccag ggcattattg ttgatatgtc ccgccatatg aaccgcatca
    37861 tcgaaattaa ccctgaagag ggctgggtgc gcgttgaggc cggggtgata aaagaccaac
    37921 tcaatcagta cctgaaaccg ttcggctact tttttgcgcc ggaactttcg accagcaacc
    37981 gggcaacgct cggtgggatg atcaataccg atgcatccgg tcagggatcg ctggtctatg
    38041 gcaaaacgtc agatcacgta cttggcgtac gcgcggtgtt gttggggggc gatattctcg
    38101 atacgcaacc tttacccgtc gagctggcgg aaacgctggg taaatccaat accacaatcg
    38161 ggcgaattta taacacggtt tatcaacgtt gccgtcagca acgccagtta atcatcgaca
    38221 acttccccaa acttaaccgc tttcttaccg gttacgatct gcgtcatgtc tttaacgatg
    38281 agatgaccga gttcgacctg acgcgcattc tgacgggttc agaagggacg ctggccttta
    38341 ttaccgaagc gcggctggat attacgcgct tgcctaaagt gcgccgtctg gtgaacgtca
    38401 aatatgactc ttttgactct gcgctgcgta acgcgccgtt tatggttgag gcgcgggcgc
    38461 tttcggtaga gacggtggac tcaaaagtgc tgaatctggc gcgggaagat attgtctggc
    38521 attccgtcag cgagttgatt accgatgtgc ctgaccaaga gatgctcggg ctgaacattg
    38581 tggaatttgc tggtgatgat gaggcgctga ttgatgagcg ggtaaatgca ctctgtgcgc
    38641 ggttggatga gctgatcgcc agtcatcaag caggtgtcat cggctggcag gtgtgccgtg
    38701 agctggcggg ggttgaacgt atctatgcaa tgcgcaaaaa agccgttggt ctgcttggca
    38761 atgccaaagg tgccgctaag ccaattccgt ttgctgagga tacctgcgta ccgccggaac
    38821 atctggccga ttatattgct gaatttcgcg cgctgctcga cagccacggc ttaagctacg
    38881 gtatgttcgg tcacgtcgac gcaggtgtct tgcacgtccg tccagcgctg gatatgtgcg
    38941 atcctcaaca agagattttg atgaagcaaa tctctgatga cgtggtggcg ctgactgcga
    39001 aatacggtgg tttgttgtgg ggcgagcacg gcaaaggttt tcgcgctgaa tacagcccgg
    39061 cgtttttcgg tgaggaactt tttgcagaac tgcgcaaagt gaaagcggca tttgacccgc
    39121 ataaccgact caacccaggg aagatttgcc cgccagaagg tctcgatgcg ccgatgatga
    39181 aagtggacgc ggtgaagcgc ggtatattcg atcggcagat ccccattgcg gtacgccagc
    39241 agtggcgcgg tgcgatggag tgtaacggca acggtttatg cttcaacttt gatgcccgta
    39301 gtccgatgtg tccgtcgatg aagatcaccc agaaccggat tcattcaccg aaagggcgcg
    39361 caacgctggt gcgtgaatgg ctgcgtttgt tggcggatcg cggcgttgac ccactcaaac
    39421 tggaacaaga actgcctgaa tccggcgtca gtttgcggac gttaattgcc cgcacgcgca
    39481 atagctggca tgcgaataaa ggcgaatatg acttctcaca cgaagtcaaa gaggcgatgt
    39541 cgggctgtct ggcctgtaaa gcgtgttcga cccagtgccc catcaaaatt gatgtgccgg
    39601 agtttcgctc tcgtttcctg cagctctatc acacccgtta tttacgcccg ctgcgcgacc
    39661 acctcgtcgc tacggtcgag agctacgcac cgctgatggc acgcgcgccg aaaaccttta
    39721 acttcttcat taaccagccg ctggtgcgca aactctcgga aaaacatatc ggcatggttg
    39781 atttgccact gctgtcggtc ccctcgctac aacaacaaat ggtggggcat cgctcggcaa
    39841 acatgacgct ggaacagctt gaagctctca atgcagagca gaaagcgcgc acggtgttgg
    39901 tggtgcagga cccctttacc agctattacg atgcgcaagt ggtggcggat tttgtccgtc
    39961 tggtcgaaaa attaggtttc cagcctgtgt tactgccatt ttcaccaaat ggcaaagccc
    40021 agcatatcaa aggctttctt aatcgttttg cgaagacggc gaaaaagacg gcggatttcc
    40081 tcaaccgtat ggcgaagctg ggtatgccaa tggtaggcgt cgatccggcg ctggtacttt
    40141 gttatcgcga tgaatataaa ctggcactgg gcgaggaacg tggcgagttt aacgtcttac
    40201 tggcgaatga atggctggca agcgcacttg agtcacagcc agtggctaca gtcagcggtg
    40261 aatcatggta tttctttggt cactgtaccg aagttaccgc cttgccgggt gcgccagcac
    40321 aatgggccgc gatatttgcc cgttttggcg cgaaactgga aaatgtcagc gtgggttgct
    40381 gcggcatggc agggacttac ggacatgaag cgaaaaacca taaaaattcg ctcgggatct
    40441 atgagttatc ctggcatcag gctatgcagc gactgccgcg taaccgctgt ctggcgaccg
    40501 gatattcctg ccgtagtcag gtcaaacggg ttgaaggcac gggggtacgc catcctgtgc
    40561 aggctttact ggagattatt aaatgatatg gaaacgaaaa atcaccctgg aagcactgaa
    40621 tgccatgggt gaaggaaaca tggtggggtt actggatatt cgctttgaac atattggtga
    40681 tgacaccctt gaagcgacaa tgccagtaga ctcgcggaca aagcagcctt tcgggttgct
    40741 gcatggaggc gcatccgtgg tgctggccga aagtatcggt tccgttgccg gttatttatg
    40801 taccgaaggt gagcaaaaag tggttggtct ggaaatcaat gctaatcacg tccgctcggc
    40861 acgagaaggg cgggtgcgcg gcatatgtaa accgttgcat ctcggttcgc gtcaccaggt
    40921 ctggcagatt gaaatcttcg atgagaaagg gcgtttgtgc tgttcgtcac gattgacgac
    40981 cgccattttg tgacgttgtc accagaaaag tgtgacgcat attgcttttg gtcattatct
    41041 gaacagtaaa cggtggtata ctgtgcaggt cttgtctaaa agcgaggatg ctatgtctac
    41101 tcaacttgat cccacccagc tggccattga atttttacgc cgtgaccaga gcaatctttc
    41161 acctgcccaa tatctcaaac gtctgaaaca actagaactt gaatttgctg acctgcttac
    41221 tctctcttct gctgaattaa aagaagaaat ctactttgcc tggcgtctgg gcgtgcattg
    41281 atcctgactt aatgccgcaa gttcgccaaa tcgacctgga ttaacctccg tccaggttcg
    41341 atgaccttct taatactgct tatctaccgc ctgatattgt tcaaaaataa tactaatcgc
    41401 agtttgtgtt aaaacggcgg gttagcttta tgagattatt cttatcgccc cttcaagagc
    41461 taagccactg agagtgccgg agataagcgc cggatggggt agaaaccctt aagcctgtgt
    41521 cgcacagact taagggtttt cttatttctg aacgctgcac ttttttacct atctcttaaa
    41581 tagtcatttt caatacaact ttttcttttt catcacagcc ttaatgctcc ttagataata
    41641 accattatct aacaatgaga tacctaattc ttagcgtgcc tgttaaccca cacatcaggg
    41701 tctatgctta ttaaaagcag gcaaaaagca tgttttacgg taaagcccct gcgtttgctg
    41761 ggttgaactg ataatcatta tcactaacat gctgttatac gctgaaagcg atgaagtgag
    41821 gtaaatcgat ggacatgcat tcaggaacct ttaacccaca agatttcgcc tggcaaggct
    41881 taacgttgac acccgcagcg gcgatacaca tccgcgagct ggtggcaaag cagccaggta
    41941 tagtcggcgt gcgcttaggc gtgaagcaaa cgggctgcgc gggctttggc tatgtgctcg
    42001 acagtgttag cgagccggac aaagacgatc tgctgtttga acacgacggc gcgaagctgt
    42061 ttgtcccgct gcaagcgatg ccgtttattg atggcacgga agtcgatttc gttcgtgaag
    42121 gacttaatca gatattcaaa tttcacaacc ctaaagccca gaatgaatgt ggctgtggcg
    42181 aaagctttgg ggtataggcg gtactatgtc tcgtaatact gaagcaactg acgatgtcaa
    42241 aacctggacc ggcggcccgc tgaattataa agaaggattc ttcacccagt tagccaccga
    42301 tgagctggca aaggggataa acgaagaggt ggtgcgcgca atttcggcga agcgtaatga
    42361 gccggagtgg atgctggagt ttcgtctcaa tgcctatcgc gcatggctgg agatggaaga
    42421 accgcactgg ttgaaagcgc actacgacaa gctgaattat caggattaca gctactactc
    42481 agcaccatcg tgcggtaatt gtgacgacac ttgcgcgtct gaacctggcg cggtgcagca
    42541 aactggcgcg aacgcctttt taagtaaaga ggtggaggcg gcgtttgagc agttgggcgt
    42601 tcccgtgcgg gaaggcaaag aggtggcggt ggatgccatt ttcgactcag tttcggttgc
    42661 cactacttat cgcgaaaaac tggcggagca gggaattatt ttctgttcct ttggtgaggc
    42721 gatccacgat cacccggaac tggtgcgtaa atatctcggc accgtggtgc cggggaatga
    42781 caacttcttt gccgcgctta atgcggcggt agcctctgac ggtacgttta tttatgtgcc
    42841 taaaggcgtg cgctgcccga tggaactttc cacctatttt cgcattaacg cggaaaaaac
    42901 cgggcagttt gagcgcacca ttctggtggc cgacgaagac agctacgtca gctacattga
    42961 aggctgttcc gctccggtgc gtgacagcta tcagttgcac gcggcagtgg tggaagtcat
    43021 catccataaa aacgccgagg tgaaatattc cacggtacaa aactggttcc ctggcgataa
    43081 caacaccggc ggtattctca acttcgtcac caagcgtgct ttgtgcgaag gcgaaaacag
    43141 caaaatgtca tggacgcaat cagaaaccgg gtccgcgatt acgtggaaat atcccagctg
    43201 cattttgcgc ggcgataact ccattggtga gttttactca gtggcgctga ccagcggtca
    43261 tcagcaagcg gataccggca ccaagatgat ccacatcggt aaaaacacca aatcgacgat
    43321 tatctcgaaa gggatctctg ccggacatag tcagaacagt tatcgcggct tagtgaaaat
    43381 catgccgacg gcaaccaatg cgcgcaattt cactcagtgc gactcaatgc tgattggcgc
    43441 taattgtggg gcgcatacct tcccgtatgt cgaatgtcgc aataacagcg cacaactgga
    43501 gcacgaggca acgacatcac gtattggtga agatcaactg ttttactgcc tgcaacgcgg
    43561 gatcagcgaa gaagacgcca tctcgatgat tgttaacggt ttctgcaaag acgtgttctc
    43621 agaactgccg ctggaatttg ccgttgaagc acaaaaactc ctcgccatca gtcttgaaca
    43681 cagcgtcgga taaggaataa acatgttaag tattaaagat ttacacgtca gcgtggaaga
    43741 taaagctatc ctgcgtggat taagcctcga cgttcgtccc ggcgaagttc acgccattat
    43801 ggggccaaac ggttcgggca aaagtacctt atcggcaacg cttgccgggc gcgaagatta
    43861 tgaagtgacg ggcggcacgg ttgagttcaa aggcaaagat ttgcttgcac tgtcgccgga
    43921 agatcgtgca ggcgaaggca tctttatggc cttccagtat ccggttgaga ttccaggtgt
    43981 cagtaaccag tttttcctgc aaacggcact taatgcggtg cgcagctatc gcggccagga
    44041 aacgctcgac cgctttgatt ttcaggattt gatggaagag aaaatcgctc tcctgaagat
    44101 gccggaagat ttattaaccc gttcggtaaa cgttggtttt tccggcggcg agaaaaagcg
    44161 caacgatatt ctgcaaatgg cggtgctgga accggggtta tgcattcttg atgaatcgga
    44221 ctccgggctg gatattgacg cattaaaagt ggttgccgat ggcgtgaact cgctgcgtga
    44281 cggcaagcgc tcattcatca ttgttacgca ctaccaacgc attctcgact acatcaagcc
    44341 tgattacgtt catgtgctat atcagggacg aattgtgaaa tccggcgatt tcacgttggt
    44401 caaacaactg gaggagcagg gttatggctg gcttaccgaa cagcagtaac gcactgcaac
    44461 agtggcatca tttgtttgaa gctgaagggg tgaaacgttc cccgcaagca caacagcatt
    44521 tacaacaatt gctgcgtacc ggactgccga cacgtaaaca tgaaaactgg aaatatacgc
    44581 cgctggaagg gctgaccaat agccagtttg tcagcattgc gggggagata tccccacagc
    44641 agcgggatgc cttagcgtta acgttagacg ccgtgcggct ggtgtttgtc gatggacgtt
    44701 acgtgcctgc gctgagcgat gcaactgaag gcagcggata tgaagtgagc attaacgacg
    44761 accgtcaggg gttacccgac gctattcagg cggaagtgtt tctgcatttg acggaaagcc
    44821 tggcacaaag cgtgacgcat atcgccgtga agcgcggtca acggccggca aagccattgc
    44881 tgttaatgca tatcacccag ggcgtggcag gtgaagaggt gaacactgcc cattaccgac
    44941 atcatctgga tctggcggaa ggtgccgaag caacggtgat cgaacatttt gtcagcctga
    45001 atgatgctcg tcattttacc ggggcacggt tcactatcaa cgtcgcagcg aatgcccact
    45061 tgcagcatat caagctggcg tttgaaaacc cgctcagtca ccactttgct cataacgatt
    45121 tgttgctggc tgaggatgcc accgcattta gccacagttt cctgctgggt ggcgtagtgt
    45181 tacgacacaa caccagtacg caactcaatg gcgaaaacag cacgctgcgg atcaatagcc
    45241 tggcgatgcc ggtgaaaaac gaggtgtgtg atacccgtac ctggctggaa cacaataaag
    45301 gtttttgtaa cagccgacag ttgcacaaaa ctatcgtcag cgacaaaggc cgcgcggtat
    45361 ttaacggttt gatcaacgtc gcgcagcacg ccatcaaaac ggatggtcag atgaccaaca
    45421 acaatctgct gatgggcaaa ctggcggaag tggatacgaa accgcagctg gaaatctatg
    45481 cagatgatgt gaaatgcagc cacggcgcga cggtggggcg tattgatgat gaacagatat
    45541 tctatctgcg ctcgcgcggg atcaatcagc aggatgccca gcagatgatc atttacgcct
    45601 tcgctgccga actgacggaa gcactgcgtg atgaggggct taaacagcag gtgctggccc
    45661 gaatcggtca acggctgcca ggaggtgcaa gatgattttt tccgtcgaca aagtgcgggc
    45721 cgactttccg gtgctttcgc gtgaggtaaa cggtttgccg ctggcttatc tcgacagcgc
    45781 cgccagtgcg cagaaaccga gccaggtgat tgacgccgag gccgagtttt atcgtcatgg
    45841 ctacgcggcg gtgcatcgtg gtattcatac cttaagcgcc caggcgaccg agaaaatgga
    45901 gaacgtgcgc aagcgggcat cgctgtttat taatgcccgt tcggcggaag agctggtgtt
    45961 cgtccgcggc acgacggaag ggatcaatct ggtcgccaat agctggggca acagcaacgt
    46021 gcgggcgggc gataacatca tcatcagtca gatggagcac cacgctaaca ttgttccctg
    46081 gcagatgctt tgcgcacgcg ttggcgcaga gctgcgtgtg atcccgctca atcccgatgg
    46141 tacgttgcaa ctggagacgc tgcctacgct gtttgatgag aaaactcgcc tgctggcaat
    46201 tactcatgtc tccaacgtgc ttggcacaga aaatccactg gcggaaatga tcacgcttgc
    46261 gcaccagcat ggcgcaaaag tgctggtgga tggcgctcag gcggtgatgc atcatccggt
    46321 ggatgttcag gcgctggatt gcgactttta cgtgttctcc gggcataaac tgtatggccc
    46381 caccggaatt ggcattcttt atgtgaaaga agccttgttg caggagatgc cgccgtggga
    46441 agggggcggt tctatgatcg ccaccgtcag cctgagtgaa ggcactacct ggaccaaagc
    46501 accatggcgg tttgaagccg gtacacccaa taccgggggc atcattggtc ttggcgcggc
    46561 gctggagtat gtttcggcgc tggggcttaa taacatagcc gagtatgaac agaatctgat
    46621 gcattacgcg ctatcacagc tggaatctgt accggatctc actctctatg gcccacaaaa
    46681 caggcttggc gttattgctt ttaatctcgg taaacaccac gcctatgatg ttggcagttt
    46741 tctcgataat tacggcattg ctgtgcgtac cggacatcac tgcgctatgc cattaatggc
    46801 ctattacaac gtccctgcga tgtgtcgggc gtcgctggcc atgtataaca cccatgaaga
    46861 agtggatcgt ctggtgaccg gattgcaacg tattcaccgt ctgctgggat aacagggagg
    46921 cactatggct gtattgccgg ataaagaaaa attgctgcgt aattttttac gctgcgccaa
    46981 ctgggaagag aaatatctct acattattga gctgggccag cgtctgccag aattacgcga
    47041 cgaagaccga agcccacaaa atagcattca gggctgccag agtcaggtgt ggattgtcat
    47101 gcgccagaat gcgcagggaa ttattgaatt gcagggcgac agcgatgcgg cgattgtgaa
    47161 agggcttatt gcggtcgtct ttattctcta cgatcagatg acgccgcagg atattgtcag
    47221 tttcgatgtg cgtccgtggt ttgaaaaaat ggcgctcacc caacatctca ccccatctcg
    47281 ttcacaaggt ctggaagcga tgattcgcgt aattcgcgcc aaagccgctg cacttagcta
    47341 aactaaaggg acagctttca tccggttggc tcacattgcc ggatggtcaa tgtttacact
    47401 gacctgcaca ggagtgtttc ggtttcaggt gaacataagc gccatccgac gtttcagcgt
    47461 gagtctccgg caaatacagg aggtttacaa tgaaacgcgc gtctttgctt acactcacgc
    47521 ttatcggcgc ttttagcgcc atccaggctg cctgggcggt tgattatccg ctaccaccaa
    47581 ccggaagccg actggttggg caaaatcaaa cgtatacggt gcaggaaggg gataaaaacc
    47641 ttcaggctat tgcccgtcgt tttgatactg cggcaatgtt gatccttgaa gccaataaca
    47701 ctatcgcccc ggtgccaaaa cctggtacga cgataactat tccttcgcaa ctgttattac
    47761 ctgatgcgcc gcgtcagggg attatcgtta accttgcaga gctgcgcctt tattattatc
    47821 cgccgggaga aaatatcgtg caggtttatc cgataggtat tggcttgcag gggctggaaa
    47881 cgccggtgat ggaaacgcgt gtggggcaga aaatccctaa cccaacctgg acgcctacgg
    47941 caggcattcg tcagcgttcg ctggagcgtg gcattaaatt acctccagtc gtccctgccg
    48001 ggccaaataa cccactagga cgttacgcac tgcgcctcgc gcatggtaat ggcgaatacc
    48061 tcattcatgg caccagtgcg ccggacagcg tcggtttgcg cgtcagttca gggtgtattc
    48121 gcatgaatgc actggatatt aaagccttgt tctccagcgt gcggacggga acgccggtga
    48181 aagtgatcaa cgaaccggtg aaatattccg tggagcctaa cgggatgcgt tatgttgaag
    48241 tacatcgacc actatcggca gaagaacagc agaacgttca gacaatgcca tacacactgc
    48301 cagcaggctt tacgcaattt aaagacaata aggctgtaga tcagaagtta gtcgataaag
    48361 cgttgtatcg tcgggcaggg tatccggttg cggtgagcag tggagcaact cccacagcca
    48421 gcaatgcgcc ttcagtagag tcagcgcaga atggtgaacc agagcaaggg aatatgttac
    48481 gcgcgacgca gtagcggtaa atggcagaca aaaaaaatgg cgcacaatgt gcgccatttt
    48541 tcacttcaca ggtactatta cttgcggtat ttagtagcca tgttgtccag acgctggtta
    48601 gcacgagctg cgtcatcttt agcagcctga acgtcggaac gcattgcgtt cacgtcgttg
    48661 ctcagctggt caactttagc gttcagagtc tgaacgtcag aagacagctg atcgatttta
    48721 gcgttgctgg agcaacctgc cagcagagta gaacccagga ttaccgcgcc cagtaccagt
    48781 ttagtagctt tcattattaa taccctctag attgagttaa tctccatgta gcgttacaag
    48841 tattacacaa agttttttat gttgagaata tttttttgat gggaaggcac ttatttttga
    48901 tcgttcgctc aaagaagcat cgaacgctgg gttttagata aaaaaattga gagaaaacag
    48961 gatgcttcca tcggattcat cttagataaa gtgagattat atagtgaaat ccgatttgat
    49021 tttttattgc ttctggttat cgattaaata aaaaaagcgc ccatcagggc gcttcgatat
    49081 acaaattaat tcacaaaagc aatattacag gacgtgaaca gatgcggtgt tagtagtgcc
    49141 gctcggtacc agtgcaccag aaaccataac tacgacgtca cctttgtgtg ccagaccgct
    49201 ctgcagagcc agttctttac ccagacggta gaaatcatca gtagaagtga tctctttaac
    49261 cagctgcggc acaacgcctt tgctcagtac caactgatga gccgtttttt cgttggtggt
    49321 cagtgccagg atggtggcat ccgggaagta tttacgtact gcgcgagcag atttaccgcc
    49381 ctgagtagca accacgatca gcggagcatc cagtttttca gcagtttcaa cggcaccacg
    49441 gcataccgct tcggtaatgc gcagtttacg gttgtcattg ttgaactcga gacggctgtt
    49501 catcacgcgg tcggtacgtt cgcagatggt cgccatgata gaaaccgctt ccagcgggta
    49561 tttacctttt gcggattcac cagacagcat cactgcgtca gtaccgtcga ggatggcgtt
    49621 tgcaacgtca ccggcttctg cgcgagtcgg gcgtgggttt ttgatcatgg aatccagcat
    49681 ctgggtcgca gtgataacga ctttacgtgc acggatacat ttttcgatca tcatcttctg
    49741 ggcgaagata acttcttcta ccgggatttc tacacccagg tcgccacgcg caaccatgat
    49801 gccgtcagag gcttcgagga tttcgtcgaa gttgttgagg ccttcctggt tttcgatttt
    49861 ggagatgatg tggatgtttt cgccgccgtg cgctttcagg tgctcacgga tttcgataac
    49921 gtcagaacgc ttacgaataa aggaagcagc aacaaagtct acgccttgtt cgcaaccaaa
    49981 gatcaggtcc tgtttgtctt tttcagccag tgctggcaga gcaatggaaa cgccaggcag
    50041 gttcacacct ttgttttcgc ccaggtcacc gttgttcagc actttacaga taactttgtt
    50101 accttcaatg gcggtaactt ccataccgat cagaccatcg tcaaccagta cggtgttgcc
    50161 aacagacagg tcagtagtga aaccttcata cgttaccgca accatttcgc tgttgccgat
    50221 aacagattta tcagtggtga aagtaaaggt ctgaccagct ttcagagaaa cgtcgttacc
    50281 gccttccagt ttcatggtgc ggatttccgg acctttggta tcaagcagga tagcggcggt
    50341 tttaccagtt ttgctcatca cgttgcgcag attctgaatg cgctgaccgt gttctgcata
    50401 gtcaccatga gagaagttca gacgcataac gttcatgcca gcgtccagca ttttagctaa
    50461 catctcttca gattcggttt tcggtccgat ggtgcaaaca attttggtct ttttcatgac
    50521 agtcttagtc tttaagttga gaaggatggg agaaacttgc tttctgggcg cactgccgga
    50581 aagcgaatcc ggtattacga aagtggtggt gcctcgctct aaggataggt gacatcgaaa
    50641 aagcgtgcag aggaatgtgt gcttgtgtct cagcccaacg tgagtgcctg tttttgagtc
    50701 gttgagttct acagtccaaa gtgctgaaac cattcaagag tcaattggcg cgcattatac
    50761 gctgaaatta atccaaaagg aaaacaaaaa cagcgtttca aaaaatatac aacttgttag
    50821 acaataaatt gttatcaaag atttaacgtt ccagggaaag gttacgcaaa aacttcgctg
    50881 tttgcgcaac gtctcgtgcc ggggttgtca ctgtgaagat aagtgaaagt tcatccggtg
    50941 tataaataca tcatcgctca attgctacag ttaaaataat ttatcgcgtt atttgtctga
    51001 ttttgtttga tttttgtcta attggttaga ttgatatatg ctgatagttg gcatgataaa
    51061 taggtataaa gaatgggaaa tcggacaaaa gaggatgaac tgtaccgaga aatgtgcaga
    51121 gttgtcggta aagttgtgct ggaaatgcgc gatctggggc aggaaccaaa gcatattgtt
    51181 atcgcaggcg tattacgtac agcattagcc aataaacgca ttcagcgcag tgagctggaa
    51241 aagcaggcga tggaaaccgt cattaacgca ctggtaaaat gaccattcag gatgaggcgg
    51301 taagcttcat cctgatattc ttgcttcagt tgcggtccag ttaatcattc taaataacaa
    51361 ctgctgaaat tatacgaatt tcggtatgta ttttaattta tagcgataat gttattttta
    51421 ttttctgcaa tgaacgtcac aaataattgt cgcttaagtt aaatttattt caaatgataa
    51481 taaatatcag ttcacttata atttcttgct ttgtaatttt aattgataac gatcaatgtt
    51541 aagggctcag aataatcaca ttattttttc tcctaaccat aaaggattag tttatcggcg
    51601 ttaatctttt ctcatacact atcgggttag ggaaaatatc tggtgttggg gacagagtca
    51661 gcagcgtatt agttgaatgc ttaatatcag gctgctgaat tgtcagcact gtgtttgcga
    51721 agaggatgcg aaataaatga acccggttga tcgtccacta ttagatattg gcttaacgcg
    51781 attagaattc ttgcgtatat caggaaaagg ccttgctggt ctaacaattg cccctgcgtt
    51841 actgtcactt ttgggttgca aacaggaaga tattgatagc ggcacagtag ggttgataaa
    51901 tacccccaaa ggggtgctgg tgacccagcg agcgcgatgc acgggttgcc accgctgtga
    51961 aatctcttgt accaacttca acgatggctc agtagggaca ttcttctccc gtatcaaaat
    52021 ccatcgcaat tatttctttg gcgacaacgg ggttggctct ggcggcggcc tgtatggcga
    52081 tctcaactat accgcggaca cctgccgtca atgcaaagag ccgcaatgca tgaacgtctg
    52141 cccgattggt gcgattacct ggcagcagaa agaaggctgt attaccgtcg atcataaacg
    52201 ctgtattggc tgtagcgcct gtactacggc atgcccgtgg atgatggcca ccgtaaatac
    52261 cgaaagtaaa aaatcctcga aatgtgtgtt atgcggtgaa tgcgcaaacg cctgcccaac
    52321 aggggcgtta aaaattatcg agtggaaaga tattactgtg tgaatcttgc aaaggaaaaa
    52381 atcatggcta acggttggac aggtaatata ttaagagtca atctcacgac aggaaatatt
    52441 accctcgaag attccagtaa gtttaaaagt tttgtcggtg gcatgggctt cggctacaaa
    52501 attatgtatg acgaagtacc gccaggcacg aaacctttcg atgaagcgaa taaattagtc
    52561 tttgctaccg gcccattaac tggatctggt gccccctgta gttctcgcgt aaatatcacc
    52621 tcactttcta cttttaccaa aggaaattta gtcgtcgatg cccatatggg tggctttttt
    52681 gcagcgcaaa tgaaattcgc tggatacgac gtcattatta tcgaagggaa ggcgaaatca
    52741 ccggtatggc tgaagattaa agatgacaaa gttagcctgg aaaaagccga tttcttatgg
    52801 ggaaaaggga cgcgcgcaac gacggaagaa atttgtcgat tgaccagtcc ggaaacctgt
    52861 gtggcggcta ttggtcaggc tggggaaaac cttgttcctc tctctggcat gttgaatagc
    52921 cgtaaccaca gcggcggtgc gggaactggc gcaataatgg gttcgaaaaa cctgaaagcg
    52981 attgcggttg aagggacgaa aggggtcaac attgccgatc gtcaggagat gaagcgtctc
    53041 aatgattaca tgatgactga acttattggt gcgaataaca accatgtcgt gccaagtacg
    53101 ccacaatcgt gggcagagta ttcagatccc aagtcacgct ggacagcacg taaagggctg
    53161 ttttggggcg cggctgaagg tggtccgatt gaaacgggtg aaattccgcc aggcaatcag
    53221 aatacggtcg gctttcgtac ctataaatcc gtttttgact taggaccggc ggcagagaaa
    53281 tacacagtaa aaatgagcgg ctgccactct tgcccgatcc gttgtatgac ccaaatgaat
    53341 attcctcggg tgaaagagtt tggcgtgccc agcacaggtg gtaacacttg tgtagcaaac
    53401 tttgtccata ccaccatctt cccgaacggg ccgaaagatt ttgaagataa agacgatggt
    53461 cgtgtgattg gtaacctggt gggtctgaat ttgttcgatg actacggcct atggtgtaac
    53521 tacgggcagt tgcatcgcga ctttacttat tgttacagca aaggtgtgtt caagcgtgtt
    53581 ctgccagctg aagagtatgc agaaattcgc tgggatcaac tggaagcggg tgacgttaac
    53641 ttcattaaag atttttacta ccgtctggcg catcgtgtgg gtgagctgag tcacctggct
    53701 gatggttcat atgccatcgc agaacgctgg aatttgggtg aagagtactg gggctacgcg
    53761 aaaaataaac tctggtcgcc gtttggctat ccggttcacc atgccaatga agcgtcagcg
    53821 caggtcggtt ccattgttaa ctgtatgttc aaccgtgact gcatgacgca tacccatatc
    53881 aactttattg gttccggctt gccattgaaa ctgcaacgtg aagtggcgaa agaacttttt
    53941 ggttctgaag atgcttacga tgaaaccaaa aactacacgc caatcaatga cgcaaaaatt
    54001 aaatatgcaa agtggtcgct gttgcgggtc tgtttgcata acgccgtcac cctgtgcaac
    54061 tgggtctggc caatgaccgt ttcgccgctg aaaagccgta attaccgggg cgatctggcg
    54121 cttgaagcca atttcttcaa agcgatcacc ggcgaagaga tgactcagga aaaattagat
    54181 ttagctgcag agcgtatctt tacgttgcat cgtgcctaca cggtaaaact gatgcaaacc
    54241 aaagatatgc gtaatgaaca cgatcttatc tgttcctggg tattcgacaa ggatccgcag
    54301 atcccggtct ttactgaagg tactgacaaa atggatcgtg acgatatgca tgcttcgctc
    54361 acgatgttct acaaagaaat gggctgggac ccacagcttg gttgtccaac ccgcgaaaca
    54421 ttgcagcgtc tggggctgga agatattgcc gccgatctgg cagcacacaa tctactgcct
    54481 gcgtaaggaa tgggtaagat gaaccatcag gatgaattgc cgttagcgaa agtcagcgaa
    54541 gttgacgagg cgaagcggca atggttgcag gggatgcgtc accccgttga tacggtaacc
    54601 gaaccagaac ctgctgaaat cctggctgag ttcattcgcc agcattctgc ggctgggcag
    54661 ctggtggcgc gcgccgtgtt tctctcgcca ccctatttgg ttgcagaaga ggaactgtca
    54721 gttttgctgg aaagcattaa gcagaacgga gactacgcgg atatcgcttg tttgacggga
    54781 agcaaggatg attactatta ctcaacgcag gccatgagtg aaaactacgc ggcgatgtct
    54841 ttgcaagttg tggagcagga catttgtcgc gccattgcac acgccgttcg ttttgagtgc
    54901 cagacgtatc ctcgtcctta caaagtcgcc atgctgatgc aagcgccata ttacttccag
    54961 gaagcgcaga ttgaagccgc gatcgccgcg atggatgttg cacctgaata tgccgatata
    55021 cggcaggtgg agtcatcgac ggcggtgttg tacttgttta gcgagcgatt tatgacctat
    55081 ggcaaggctt atggcttatg cgaatggttc gaggtagagc agtttcagaa cccctgaaat
    55141 ttcagggaga tgtcatggat agggtggaca gttgcagcca ctgcattagg ggataatcag
    55201 atgtccttca ctcgacgcaa atttgttctg gggatgggaa cagtaatctt ttttactggt
    55261 tctgcgtctt ctctgttagc gaacacgagg caagaaaagg aagttcggta tgccatgatt
    55321 catgacgagt cacggtgcaa tggctgtaat atttgcgccc gcgcatgccg taaaactaac
    55381 cacgtccccg ctcagggaag ccgtttatcg atagcacaca ttccagtcac cgataacgac
    55441 aacgaaacgc agtatcactt ttttcgccag tcatgtcagc actgcgaaga tgctccgtgc
    55501 atcgacgttt gcccgacggg ggcatcgtgg cgtgatgagc aggggatcgt gcgggtagaa
    55561 aaatcgcagt gcatcggctg cagttattgc attggcgctt gtccgtacca ggttcgctac
    55621 cttaaccctg tcactaaagt ggcagataaa tgtgatttct gcgctgagtc ccggctggca
    55681 aaaggctttc cgcccatttg cgtcagcgct tgtccggaac acgcattgat ctttggtcgc
    55741 gaagatagcc cagaaattca ggcgtggttg caggaaaata aatactatca atatcagcta
    55801 cctggtgccg ggaaaccgca tctgtatcgc cggttcggtc aacatttgat taaaaaggaa
    55861 aatgtatgaa cccgtcgcaa catgctgaac agtttcagag ccagttagcg aactatgtgc
    55921 cacagttcac tcccgaattt tggccggtgt ggttgatcat tgccggagta ttgctggttg
    55981 ggatgtggtt ggtgctgggg ctgcacgcct tgcttcgtgc tcgtggcgtg aagaaatcag
    56041 ccaccgatca tggtgagaag atttatcttt acagcaaagc ggtcagatta tggcactggt
    56101 cgaatgcgtt actctttgta ttgttgctgg caagtgggct gataaatcac tttgcgctgg
    56161 tgggcgcaac tgcggttaaa agtctggttg cgttgcatga agtttgcgga tttttgttac
    56221 tcgcatgctg gctcggcttt gtgctgatca atgctgttgg gggtaatggt caccactatc
    56281 gcattcgtcg tcaggggtgg ctggagcgag cggcaaaaca aacgcgattt tatttgtttg
    56341 gcattatgca gggggaagaa catcctttcc cggcaacaac ccagtctaaa tttaatccct
    56401 tacagcaggt cgcctatgtt ggtgtcatgt atggattgct gccgttgtta ctattgacgg
    56461 ggctgctgtg tctctatccg caagccgtgg gagatgtgtt tcctggcgta agatactggt
    56521 tattgcaggc gcattttgct ctggcattta taagcctctt ttttatcttc ggtcatcttt
    56581 atctttgcac cacggggcgt acgccacacg aaacctttaa aagcatggtc gatggctatc
    56641 accggcacta acgaatgatc attacacgag cagacctgcg agaatggcgt atcggagcgg
    56701 tgatgtatcg ctggttcctg cgccatttcc cacgcggcgg tagttatgcc gatattcatc
    56761 atgcactgat tgaggaagga tatacggact gggcggagag tctggttgag tatgcgtgga
    56821 aaaaatggct ggcggatgaa aacttcgcgc atcaggaagt ttcctctatg caaaaactgg
    56881 ccacagaccc cggagcgaga ccattttgca gtcagtttgc ccgcagcgac gatcatgctc
    56941 gcatcggctg ttgtgaggat aacgccagaa tcgcaaccgc tgggtatgcc gcgcaaattg
    57001 ccagtatggg ttatagcgtg cggattggaa gtgtcggctt taacagtcac attggcagct
    57061 ccggtgaacg cgcacgggtt gctgtaaccg gtaattcttc gcggataagc agcgctggag
    57121 atagtagtcg gattgcgaat accggaatgc gagtacgcgt ttgtacattg ggtgaacggt
    57181 gtcatgtcgc cagtaatggt gacctggtgc aaatcgccag ctttggcgca aacgccagaa
    57241 ttgccaacag cggcgacaat gtacacatca ttgccagcgg tgaaaattca acggtagtga
    57301 gcacgggcgt ggtcgactca attattctcg ggccaggggg cagcgccgca ctggcatatc
    57361 atgacggcga aagagttcgc ttcgccgtcg ccattgaagg tgaaaacaat attcgagcca
    57421 gagtccgcta tcgtcttaat gaacagcatc agtttgttga gtgctgatag ctttcccttt
    57481 aatcaaacga aagacgccga cgagcacggg atgcaggctt tacgaccgcc cgagccatcg
    57541 cctcaccaat ttctgcacat gccgtaagtc cctgaacgaa aggctggtcg tgcatcaacc
    57601 agggcaacgc gccgaacgct acgcgtccac gaatatcttc gagttgctgt aacgtataat
    57661 cttcgccaac atcagggatt tcatcccctg ttttctgtaa ttgttcgcgt agtccatgga
    57721 aaggaatatc tttcacttta agcggacgct gcccgcgggc atcaataaaa acgtcaaacg
    57781 agtagctgtt gtcttccgtt ttcaggacgg tgcgcgactc attaatttcc attttgtagt
    57841 cttcaccgag ggcgagaata tgaatgattc ccgcttcacg taaggcaagt aggcgacgaa
    57901 tagactctga agggattgcc gcataattat cgatgaaaac ccgggcaagg cctttactga
    57961 accgtttatg gtcgtgttca ttcagatgtg gaacaatttc ctgtacggct tcatgcaggc
    58021 gcagaatgac ataacgccag ggaacagtat gtttttctcg tttattgcgt tcgacttcct
    58081 ggagattttt ttctgcccag tcaaatgggt cgcgttgttt gcgctcggca aaccaggctt
    58141 gagcaaagga atcgacattc aggctctcta aggctatgcg ttgactccag tctggatcag
    58201 caaacttgat ttcctctact atcaatctaa atacccgatc caaaaggccc tcttcgcctt
    58261 tttgaatctc agcatttaat gcctgatcgg tgacgatgtg taagggctcg tagggaatag
    58321 ggcaatagaa atcggcttcg ggtaaaatac ccgtgcgcga catcaacgtg atatttagct
    58381 tttcacttgc gttatcgcgg tgaaaaacga cgtgttgttt atcatcttca atgaacgaac
    58441 catgctgaat agccactgcc attgccgcat ccagtccgct caaggatgtt cccataatac
    58501 ccacgttaca cgcatcgacc tttgcttcca tcaggcctga ccacgggctg ggaaaatacg
    58561 ttcgggttgc ttcttcttca tcaggccaga cgtgacccgt ggcaatcacc gctaaatcaa
    58621 acgtctcgct gggtaaatcc tgatttgtag cgagcatgac gccaacattt gtaatttgca
    58681 gatcggtaac ctggcatgat tcataaacag ccactgcaaa tttttgcttt cgtgcctggt
    58741 ctactaatcg taaaaattga tcgcggaaat attcgcccag cagaattcgc ggtaaaaact
    58801 gacgatcgtg caaggtttct tttttaacgc cataacgctg gagatggctg gcttcttgct
    58861 tttgtagcca ttcgagatac gtacaataaa tcggcggtat ttcaatactg gcaatatttg
    58921 ccagcatcat ttttgagttt tcctcatcac tgtatggcat cccgacaccg gcctcgtcag
    58981 cctgctcgaa gatagaaata gaaagtggag tttgttgctg taga
//
LOCUS       DAOFQD010000012        56075 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.012, whole genome shotgun sequence.
ACCESSION   DAOFQD010000012 DAOFQD010000000
VERSION     DAOFQD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.012"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(5..457)
                     /gene="tabA"
                     /locus_tag="QBF46_000780"
     CDS             complement(5..457)
                     /gene="tabA"
                     /locus_tag="QBF46_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDQ6673007.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(602..1195)
                     /locus_tag="QBF46_000781"
     CDS             complement(602..1195)
                     /locus_tag="QBF46_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDQ6673008.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            1266..1979
                     /gene="bdcA"
                     /locus_tag="QBF46_000782"
     CDS             1266..1979
                     /gene="bdcA"
                     /locus_tag="QBF46_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6673009.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYARSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2110..2505
                     /locus_tag="QBF46_000783"
     CDS             2110..2505
                     /locus_tag="QBF46_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDQ6673010.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2786..2920
                     /gene="pyrL"
                     /locus_tag="QBF46_000784"
     CDS             2786..2920
                     /gene="pyrL"
                     /locus_tag="QBF46_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDQ6673011.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2924..3859
                     /gene="pyrB"
                     /locus_tag="QBF46_000785"
     CDS             2924..3859
                     /gene="pyrB"
                     /locus_tag="QBF46_000785"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDQ6673012.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3872..4333
                     /gene="pyrI"
                     /locus_tag="QBF46_000786"
     CDS             3872..4333
                     /gene="pyrI"
                     /locus_tag="QBF46_000786"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDQ6673013.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4406..4792
                     /gene="ridA"
                     /locus_tag="QBF46_000787"
     CDS             4406..4792
                     /gene="ridA"
                     /locus_tag="QBF46_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDQ6673014.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGSMIITSGQIPVNPKTGEVPT
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            4858..5313
                     /locus_tag="QBF46_000788"
     CDS             4858..5313
                     /locus_tag="QBF46_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6673015.1"
                     /translation="MAKPRFTDEQIAEILQQSKEGASNKELCEHYQFSVSTLRRWQEQ
                     HADGIRSELKKTESKAQIVFLVFFAIAILLTLIFDKPTGGWVIPPLLIYCVYYIRQYR
                     NISGRHIKKEDIYLSRSVNNSYSALYNLSWTFICFFIFAVIYFFIQVFS"
     gene            complement(5358..8054)
                     /gene="mgtA"
                     /locus_tag="QBF46_000789"
     CDS             complement(5358..8054)
                     /gene="mgtA"
                     /locus_tag="QBF46_000789"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDQ6673016.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESACEQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8195..8248)
                     /gene="mgtL"
                     /locus_tag="QBF46_000790"
     CDS             complement(8195..8248)
                     /gene="mgtL"
                     /locus_tag="QBF46_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDQ6673017.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8433..9380
                     /gene="treR"
                     /locus_tag="QBF46_000791"
     CDS             8433..9380
                     /gene="treR"
                     /locus_tag="QBF46_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDQ6673018.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            9499..10920
                     /gene="treB"
                     /locus_tag="QBF46_000792"
     CDS             9499..10920
                     /gene="treB"
                     /locus_tag="QBF46_000792"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDQ6673019.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10970..12625
                     /gene="treC"
                     /locus_tag="QBF46_000793"
     CDS             10970..12625
                     /gene="treC"
                     /locus_tag="QBF46_000793"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDQ6673020.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFSRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13019..15157
                     /gene="nrdD"
                     /locus_tag="QBF46_000794"
     CDS             13019..15157
                     /gene="nrdD"
                     /locus_tag="QBF46_000794"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDQ6673021.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15337..15801
                     /gene="nrdG"
                     /locus_tag="QBF46_000795"
     CDS             15337..15801
                     /gene="nrdG"
                     /locus_tag="QBF46_000795"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDQ6673022.1"
                     /translation="MNFHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15846..16232)
                     /gene="cybC"
                     /locus_tag="QBF46_000796"
     CDS             complement(15846..16232)
                     /gene="cybC"
                     /locus_tag="QBF46_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDQ6673023.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AVQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16416..17768)
                     /gene="pmbA"
                     /locus_tag="QBF46_000797"
     CDS             complement(16416..17768)
                     /gene="pmbA"
                     /locus_tag="QBF46_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDQ6673024.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPYAGVADKELLAFDAPDLDLFHPADVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17862..18413
                     /locus_tag="QBF46_000798"
     CDS             17862..18413
                     /locus_tag="QBF46_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDQ6673025.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18496..19869)
                     /gene="mpl"
                     /locus_tag="QBF46_000799"
     CDS             complement(18496..19869)
                     /gene="mpl"
                     /locus_tag="QBF46_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDQ6673026.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20045..21043
                     /gene="fbp"
                     /locus_tag="QBF46_000800"
     CDS             20045..21043
                     /gene="fbp"
                     /locus_tag="QBF46_000800"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDQ6673027.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21076..22071)
                     /gene="yjfF"
                     /locus_tag="QBF46_000801"
     CDS             complement(21076..22071)
                     /gene="yjfF"
                     /locus_tag="QBF46_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDQ6673028.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22058..23080)
                     /locus_tag="QBF46_000802"
     CDS             complement(22058..23080)
                     /locus_tag="QBF46_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6673029.1"
                     /translation="MPQSLPDTTPSKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGG
                     RNRDKT"
     gene            complement(23094..24596)
                     /locus_tag="QBF46_000803"
     CDS             complement(23094..24596)
                     /locus_tag="QBF46_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDQ6673030.1"
                     /translation="MNTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAQRFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(24734..25690)
                     /gene="ytfQ"
                     /locus_tag="QBF46_000804"
     CDS             complement(24734..25690)
                     /gene="ytfQ"
                     /locus_tag="QBF46_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDQ6673031.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26000..26530
                     /gene="ppa"
                     /locus_tag="QBF46_000805"
     CDS             26000..26530
                     /gene="ppa"
                     /locus_tag="QBF46_000805"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDQ6673032.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26609..26959)
                     /gene="chpB"
                     /locus_tag="QBF46_000806"
     CDS             complement(26609..26959)
                     /gene="chpB"
                     /locus_tag="QBF46_000806"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDQ6673033.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAAGE
                     VVEEALLRLQAVVE"
     gene            complement(26953..27204)
                     /gene="chpS"
                     /locus_tag="QBF46_000807"
     CDS             complement(26953..27204)
                     /gene="chpS"
                     /locus_tag="QBF46_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDQ6673034.1"
                     /translation="MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGDEIW"
     gene            complement(27416..27757)
                     /locus_tag="QBF46_000808"
     CDS             complement(27416..27757)
                     /locus_tag="QBF46_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDQ6673035.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(27760..31539)
                     /gene="tamB"
                     /locus_tag="QBF46_000809"
     CDS             complement(27760..31539)
                     /gene="tamB"
                     /locus_tag="QBF46_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDQ6673036.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVSI
                     NLVTLNAKGSEQQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIWPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGLLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGGAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(31536..33269)
                     /gene="tamA"
                     /locus_tag="QBF46_000810"
     CDS             complement(31536..33269)
                     /gene="tamA"
                     /locus_tag="QBF46_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDQ6673037.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKSRETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33475..34113
                     /gene="msrA"
                     /locus_tag="QBF46_000811"
     CDS             33475..34113
                     /gene="msrA"
                     /locus_tag="QBF46_000811"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDQ6673038.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34436..35779
                     /locus_tag="QBF46_000812"
     CDS             34436..35779
                     /locus_tag="QBF46_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDQ6673039.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(35841..36047)
                     /locus_tag="QBF46_000813"
     CDS             complement(35841..36047)
                     /locus_tag="QBF46_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDQ6673040.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36372..36929
                     /locus_tag="QBF46_000814"
     CDS             36372..36929
                     /locus_tag="QBF46_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDQ6673041.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(36919..37659)
                     /gene="cysQ"
                     /locus_tag="QBF46_000815"
     CDS             complement(36919..37659)
                     /gene="cysQ"
                     /locus_tag="QBF46_000815"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDQ6673042.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37849..39792
                     /gene="cpdB"
                     /locus_tag="QBF46_000816"
     CDS             37849..39792
                     /gene="cpdB"
                     /locus_tag="QBF46_000816"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDQ6673043.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALVGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39921..40301)
                     /locus_tag="QBF46_000817"
     CDS             complement(39921..40301)
                     /locus_tag="QBF46_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDQ6673044.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40390..41250
                     /gene="qorB"
                     /locus_tag="QBF46_000818"
     CDS             40390..41250
                     /gene="qorB"
                     /locus_tag="QBF46_000818"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDQ6673045.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAADVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41358..42323
                     /locus_tag="QBF46_000819"
     CDS             41358..42323
                     /locus_tag="QBF46_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ6673046.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDLARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWPNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42431..43093
                     /gene="ytfE"
                     /locus_tag="QBF46_000820"
     CDS             42431..43093
                     /gene="ytfE"
                     /locus_tag="QBF46_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDQ6673047.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(43138..44631)
                     /locus_tag="QBF46_000821"
     CDS             complement(43138..44631)
                     /locus_tag="QBF46_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673048.1"
                     /translation="MLPIKPVCLALALFSLNSIACSGVPSASFYYQPWGQGTKYIVED
                     SDGYGLYFPDIHLPQVRFKQLRRVSDGTDDEYDSPDDINNDFVRVDGGAYIRTNGGWR
                     FDWLTDGRHILWAGKIVQNPPGTPKVDAATFRAWGRFAADKDSLYFDGERTDDNHGEK
                     QVDMDSLQQVGGRMNSLDSADVLKGRRNLYFQGRWLGSAQGYSILDIKPWTRRPIIYS
                     PNSCESKSNPGPWDTIWRTKSQVFVNGDAINADPDTFHIVRWIAGSLLIYRDKDGEKR
                     YAFGKNCRSIFDLQRDKVTWMTRDASRRANDCRVATIPDVDPEYFRPLTENVVQYKDS
                     LYLVKYISAVEKTLSVIHLPDPQQELQKGANVVGNKVYFIARDDVTIFDINGQWQWYK
                     SPNGTPLNYLAHDDRYTYFIDEDTVEHFELKGQWTWFKYANGQLSDTFAHDDHYIYYV
                     GDGLVRDAKRRNETRQLDAAHLDKNGSLLTVEGKYTSYNNELLPLND"
     gene            complement(44664..45821)
                     /locus_tag="QBF46_000822"
     CDS             complement(44664..45821)
                     /locus_tag="QBF46_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673049.1"
                     /translation="MVKRSWLLIAALPPSISPSWGADFYYRQQEKGTVYVVEQKGEKD
                     EILSELPDINFSRLWRIANLANKQDSRLLSDFNPDKFDCDDEGDCEHAWLTDGRSVLW
                     SGKVLKNPPGKPIVDAASFQAFGAFAADKRSIYFDGQRTDDNSGDKQVDMSTLEETDI
                     WNLLRDKNSLWHKGHWLGSADGFQILRHDSSLQFVVQTNSQVIVNGKPLPADRKTFQI
                     KRWMPGERLVYRDKSGERDYELEDTSYRCAPFNIGLNNVSWLKYEATPAGSECIYETL
                     AGVDPEYFYLFVRNTGLYKNQIYKVTINALGEGELVNLKPEDLSDSLEAGGSWGLTNT
                     FISTDGQLYTQQATGIGKEHAQQGEWLRYNLGKGGWLSVKQPPSGLKPLFK"
     gene            complement(45955..47358)
                     /gene="cycA"
                     /locus_tag="QBF46_000823"
                     /gene_synonym="dagA"
     CDS             complement(45955..47358)
                     /gene="cycA"
                     /locus_tag="QBF46_000823"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDQ6673050.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(47667..48287)
                     /gene="fklB"
                     /locus_tag="QBF46_000824"
     CDS             complement(47667..48287)
                     /gene="fklB"
                     /locus_tag="QBF46_000824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDQ6673051.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            48505..49143
                     /gene="ytfB"
                     /locus_tag="QBF46_000825"
     CDS             48505..49143
                     /gene="ytfB"
                     /locus_tag="QBF46_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDQ6673052.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVMPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQITQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGNFIRAR"
     gene            complement(49144..49782)
                     /locus_tag="QBF46_000826"
     CDS             complement(49144..49782)
                     /locus_tag="QBF46_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDQ6673053.1"
                     /translation="MYLDTSHAQNLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIREKERELDISGLSPVIAIEKIIDFTWDYYINNP
                     WFLKIVHSENQSKGVHYKKSKRLAEINHAHLQSMASLLEEGQRQKTFKKDIDPLQINI
                     NIAALGAYYLINQYTLGMVYHISMVSPEALEARRRVIKETILSWLLVNPARG"
     gene            49992..50783
                     /locus_tag="QBF46_000827"
     CDS             49992..50783
                     /locus_tag="QBF46_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDQ6673054.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARSGFNLLINDRPDADSVE
                     KLHITQQECMAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            50793..51635
                     /locus_tag="QBF46_000828"
     CDS             50793..51635
                     /locus_tag="QBF46_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDQ6673055.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSENLQQIARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRLLAFHVSDWLIPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            51645..52421
                     /locus_tag="QBF46_000829"
     CDS             51645..52421
                     /locus_tag="QBF46_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDQ6673056.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPHAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            52431..53972
                     /locus_tag="QBF46_000830"
     CDS             52431..53972
                     /locus_tag="QBF46_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDQ6673057.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTEDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELAAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIRHPRLMESSLFT
                     PMEDA"
     gene            53969..56041
                     /locus_tag="QBF46_000831"
     CDS             53969..56041
                     /locus_tag="QBF46_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDQ6673058.1"
                     /translation="MSQLDDTILDALTYVTFPKGFASAEPTSIVTVEDVDYPIWQVDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLQEEVQRLAIPVLTSATVIKLLHQRDENGEDRVVGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEPVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADRVRRATRDALLLNEFV
                     QRHGLAIKHVGEVSELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGVKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYTQ"
BASE COUNT        13753 a        14842 c        14031 g        13449 t
ORIGIN      
        1 gtcatcacgc catcagcatc ttcactactg ctttgcgaac ctgggctggt gcacccactg
       61 cgcacagcgg tttatgcact tcccccggat aaaacacaac aaaatcacct tcattaagga
      121 taactgtttt ctcatcaacg ccttccggca aaaatgcgat gtctttatca gctaaccagt
      181 cggtatccgg cgtgcctgca ggttgcgtgc tgaaggtcat gccttcctga ccttttaaca
      241 caatctgaat gtcgagatag cgggcatggt actccgcacg gcgggcttcg tacggctcgg
      301 tcatatcttc cgagataaga taaaacagac gattgccttc gatatcgtgc ttgccctttg
      361 gcgtttctgc cgtaacgtgt gctttgatat gctcaatcgc ctggcgtaac tcctggggta
      421 gccacggctg aagattatga atatttccga tgatcatctt ttccactcct taattaaaac
      481 aacgtttcat ttctatcgtt atacgaaaag atgatcaatt ccgccagcct taacgcgtga
      541 attattgtct tatcacaact tatcggcagg cgcgttcccg cctttcggat caaggccagc
      601 atcactcctt gagaagagtt tttatcgctt cccccgccag tgcagctgtt gcacagagtt
      661 gttcgattga gtgcccctcc cgtgccttcg cagaaagccc tgacatcacg gtactcataa
      721 aatcagtcac acattgtgcg ctttgtggat gccgcctggc gatatagtca taaatggtcg
      781 tttctgcggc gtgataatat tgaacggcaa tatcacgcgc tagtggatca tgactatgaa
      841 taccttcaag aaccatacag ccagcgcagc cgccgttttg gctatatctg cgcgccgctt
      901 cttttaatac ctcaaccagg cactcgccta ctggacgatc atcacgaaga atatcggcaa
      961 gcggaatagc ttccgtaccg acgtattcat tgagtacacg gctaaataac ccagctttac
     1021 tgccaaaagc cgcgtagagg ctcggggggt taataccaag ataatcagta acttcagcaa
     1081 cactgacagc atcgaaacct ttttggtgaa aaagcacttt tgccgcagag attgcctgct
     1141 caggagcgaa acgtctggga cgacctggaa cgcgagattg ttttttagtg accattacaa
     1201 aacctgttga cagaaagtta aaacagtttt gtaatgcatg ttacataata aatcaaggag
     1261 tccttatggg cgcttttaca ggtaagacag ttctcatcct cggtggcagt cgtggtatcg
     1321 gtgccgctat cgtacgtcgt ttcgtcaccg atggggccaa tgtacgattc acctatgcga
     1381 ggtcgaaaga tgccgctaaa cgcctggcac aagagactgg agcgacagca gtattcacag
     1441 atagtgctga cagagacgct gtcattgatg tcgttcgtaa gagcggcgca ttggatatcc
     1501 tggtggtaaa tgcaggtatt ggcgtctttg gcgaggccct ggaattaaat gccgacgata
     1561 ttgatcgcct tttcaaaatc aatattcatg ctccttatca tgcctctgtt gaagccgccc
     1621 ggcagatgcc cgaaggcggg cgcatcttaa tcatcggctc cgtgaatggc gatcgtatgc
     1681 ctgttgcagg catggctgct tatgccgcca gcaaatctgc cctgcaaggc atggcgcgcg
     1741 ggctggcccg tgattttgga ccgcgtggga tcaccattaa cgtcgtccag ccagggccaa
     1801 ttgataccga cgctaatccc gccaacgggc caatgcgcga tatgttgcat agtttgatgg
     1861 ctatcaaaag acatgggcaa ccggaagagg tcgctggtat ggtcgcatgg ttagcagggc
     1921 cagaagccag ttttgttacc ggcgcgatgc ataccattga tggcgcgttt ggcgcataac
     1981 cgactacgct caattaagcc cagccattat ccatgatgtc tgggttttgt ttactcacgt
     2041 cgtccgctaa aagcggctcc cggtaaagag aaatcttctg ttcatgtcgt aaatcttgag
     2101 gataaccaaa tggtagaaag aaccgctgtt ttccctgctg gccgacattc actatatgct
     2161 gagcatcgtt attctgcggc tattcgttcc ggcgatttgc tgtttgtttc cgggcaagta
     2221 ggaagtcgag aggacggaac accagaaccc gattttcagc aacaagtcag actggcattt
     2281 gataatttgc atgcgaccct ggcagctgcg ggatgcactt ttgacgatat cattgatgtt
     2341 acgagcttcc ataccgatcc agaaaaccaa tttgaagaca tcatgacggt gaaaaatgaa
     2401 atatttagcg ccccacctta tccaaactgg acggcggtgg gtgttacatg gctggcaggc
     2461 tttgattttg aaattaaagt gatagcgcgc atccctgagc agtaagcaat agtgttagcc
     2521 gttcgctttc acactccgcc ctataagtcg gatgaatgga ataaaatgca tatctgattg
     2581 cgtgaaagtg aaaaaggaaa aagcagggaa tgtctgcaat tattgatacc gaaggacagt
     2641 tcccctgcag aatcacatca aataaaaatg catatacctt gacttttaat tcaaataaac
     2701 cgtttgcgct gacaaaatat tgcatcaaat gcttgcgccg cttctgacga tgagtataat
     2761 gccggacaat ttgccgggag gatgtatggt tcagtgtgtt cgacattttg tcttaccgcg
     2821 tctgaaaaaa gacgctggcc tgccgttttt cttcccgttg atcacccatt cccagcccct
     2881 caatcgaggg gctttttttt gcccaggcgt caggagataa aagatggcta atccgctata
     2941 tcagaaacat atcatttcca taaacgacct tagtcgcgat gaccttaatc tggtgctggc
     3001 gacagcggcg aaactgaaag caaacccgca accagagctg ttgaagcaca aagtcattgc
     3061 cagctgtttc ttcgaagcct ctacccgtac ccgcctctct ttcgaaacat ctatgcaccg
     3121 ccttggtgcc agcgtggtgg gcttctccga cagcgccaat acatcactgg gtaaaaaggg
     3181 cgaaacgctg gccgatacca tttcggttat cagcacttac gtcgatgcga tagtgatgcg
     3241 tcatccgcag gaaggtgcgg cgcgcctggc caccgagttt tccggcaatg taccggtact
     3301 gaatgccggt gatggctcca accaacatcc gacgcaaacc ttgctggact tattcactat
     3361 tcaggaaacc caggggcgtc tggacaatct ccacgtcgca atggttggtg acctgaaata
     3421 tggccgcacc gttcactccc tgactcaggc gttagcgaag ttcgacggca accgttttta
     3481 cttcatcgcg ccggacgcgc tggcaatgcc gcaatacatt ctggatatgc tcgatgaaaa
     3541 agggatcgca tggagtctgc acagctctat tgaagaagtg atggcggaag tagacatcct
     3601 gtacatgacc cgcgtgcaaa aagagcgtct ggacccgtcc gagtacgcca acgtgaaagc
     3661 gcagtttgtt cttcgcgcca gcgatctcca caacgccaaa gccaatatga aagtgctgca
     3721 tccgctgccg cgtgttgatg agattgcgac ggatgttgat aaaacgccac acgcctggta
     3781 cttccagcag gcaggcaacg ggattttcgc tcgccaggcg ttactggcac tggttctgaa
     3841 tcgcgatctg gtactgtaag gggaaataga gatgacacac gataataaat tgcaggttga
     3901 agctattaaa cgcggcacgg taattgacca tatccccgcc cagatcggtt ttaagctgtt
     3961 gagtctgttc aagctgaccg aaacggatca gcgcatcacc atcggtctga acttgccttc
     4021 tggcgagatg ggccgcaaag atctgatcaa aatcgaaaat acctttttga gtgaagatca
     4081 ggtagatcaa ctggcattgt atgcgccgca agccacggtt aaccgtatcg acaactatga
     4141 agtggtgggt aaatcgcgcc ccagtctgcc agagcgcatc gacaatgtgc tggtctgccc
     4201 gaacagcaac tgtatcagcc atgccgaacc ggtttcttcc agctttgccg tgcgaaaacg
     4261 cgccaatgac atcgcgctca aatgcaaata ctgtgaaaaa gagttttccc ataatgtggt
     4321 gctggccaat taattgcggt tggtaataaa agtctggctc cctataatga gccagacttt
     4381 ttaccgctgt aataaaggag aaatcatgag caaaactatc gcgacggaaa atgcaccggc
     4441 agctatcggt ccttacgttc agggcgttga tctgggcagc atgatcatca cttccgggca
     4501 gatcccggta aacccgaaaa cgggcgaagt accgacagac gtcgcagcac aggcacgtca
     4561 gtcgctggat aacgtaaaag caatcgtcga agccgctggc ctgaaagtgg gcgacatcgt
     4621 taaaaccacc gtgtttgtga aagatctgaa cgacttcgca accgtaaacg ccacttacga
     4681 agccttcttc accgaacaca acgccacctt cccggcacgt tcttgcgttg aagttgcccg
     4741 tctgccgaaa gacgtgaaga ttgagatcga agcgatcgct gttcgtcgct aatctctact
     4801 gaaagataat ccaggtattt cgcctgggtt atctgattca accacggagg gttgaagatg
     4861 gctaaacccc gtttcactga cgaacaaatc gctgaaattt tacagcagtc aaaagagggg
     4921 gcgtctaata aggagctatg tgaacactat caatttagcg ttagcacgct gcggcgctgg
     4981 caagaacagc acgctgacgg catcagaagc gaattaaaaa aaacagaatc caaagcacaa
     5041 atcgtctttc tggttttctt tgcaatcgct attctactga ctcttatatt tgacaaacct
     5101 acaggtggat gggtaattcc acctttatta atctattgtg tttattatat ccgccagtat
     5161 cgcaacatct ctggcagaca tattaaaaaa gaagatatct atctctctcg ttcagtaaat
     5221 aacagctata gcgcccttta taatttgagc tggacgttta tttgtttttt tatttttgca
     5281 gtaatttatt tttttattca ggtgttttcg taaaagactg acctgaaaga gtatttcagg
     5341 ccagcctcat cacttgctta ctgccagccg taacgacggc tataaaatcc tttcaccaac
     5401 tgggttaacg tcatataccc tgccaaaatc gcaaccagcc acgggaaata gcttaacggc
     5461 agcgcctgca gctgcagata actggccagc ggtgaaaacg gcaatgcgat cccgacaatc
     5521 atcacgatca cggtcatgat cattaacggc cacgatgcac agctctgaat aaacggcaca
     5581 cggcgggtgc ggatcatatg cacaatcagc gtttgcgaca gtaagcccac cacaaaccat
     5641 cccgactgga acagcgtttg cgtttccggc gtgttggcat ggaataccca ccacatcagg
     5701 caaaacgtca gaatatcgaa gatcgagctg atcggtccga agaagatcat aaagcgcccc
     5761 agatccgccg gattccaacg ctgcggcttt tgaatttgct cgtcgtcgac gttatcaaac
     5821 gggatcgcca cctgtgacac atcatacagc aggttctgaa taagcaagtg taacggcaac
     5881 atcggcagga agggcaagaa agcactcgcc accagcacgc tgaacacatt accgaagttg
     5941 gagctcgccg tcattttgat gtatttcagc atgttggcga aagtgcgacg tccttcaata
     6001 accccctctt ccagcaccat caggcttttt tccagcagga tgatatcagc cgcttcacgg
     6061 gcaatatcta ccgcgccgtc cacagaaatg ccgatatccg cagcgcgtaa ggcgggcgca
     6121 tcattaatac catcgcccat aaagccaacc acatgccctt cgcgcttcag taaggtcaca
     6181 atgcgttctt tatgcatcgg cgtcaggcgg gcaaacagcg tggtgcgctg tgcgagattt
     6241 gccagttcat cgtcagatag cgtttcaata tcactaccaa tgaccacctc tcccgcgtcc
     6301 agccccactt catggcacac tttcgctgca actaactcac tgtcgccagt aaggattttt
     6361 acggtgattc cactcgcttt taatgccttc agtgccggag cggttgtctc ttttggcgga
     6421 tcaaggaaag caatatatcc ttcgaggatc aggtcggatt catccgcccg ctggtaatct
     6481 ccttcacgcg ctggcaggta tttcgtcgcc accgcaacca cgcgcagccc ctgacgatta
     6541 agcgtatcag taacccgctt aatcttacgc agcatgatgt catcgagcgg cacaatctcg
     6601 ccattgtgac gcacctgcga acacacattg aggatttcct gcaatgcacc tttgcaaacc
     6661 agctgatggt gctcggtatt ttctgccact accaccgaca tccggcgacg ctcgaaatca
     6721 aacggaatct catcaatttt ttgccaacga ctggccagcg agcgcgctga ctcttcatcc
     6781 gtaccttcga gcaccgctgt atcgagcagg tttttaagtc cggtctgata atgactgttc
     6841 aaccacgcgc tatgcagcac gcgttcgctg gttttaccgg agatatcggt atgattctcc
     6901 agcacaattt tgtcctgtgt cagggtgcca gttttatcag tgcacagaat atccattgcg
     6961 ccaaagttct gaatagcatc cagatgtttg acgatcactt tctgtttcga cagctttact
     7021 gcgccgcgcg ccagcgtcga ggtaacaatc atcggcaaca tttccggcgt taagcctacc
     7081 gcgaccgaaa gcgcaaacag tgccgcttcc caccagtcgc ctttggtgta accattgatt
     7141 aacagcacca ccggagccat caccagcata aagcgaatca gcagcatact gacgcggctg
     7201 atcccttgct gaaaggcatt cggctcgctt tcctgctcac taacacgccc cgccagttga
     7261 ccaaaccagg tattggcacc tgtagcaatc accattgctt gtgccgtgcc gctcaccacg
     7321 gtggtgccca taaaacacag ggtgtcacac tccagcggat tgctgtgctc cggctggcga
     7381 gtggtagcgg ctttttctac gggcagagac tcaccggtta acgacgcctg agcgacgaac
     7441 agatcccgcg cctgcaagat acgtaaatct gccgggatca tatcccccgc cgccagtttt
     7501 ataatatcgc cgggcaccag ctggtcgatc gggatctcca gccagccatt ttcgcctttg
     7561 tcgttaatta cgcgcagcac cgtcgcggta ttgctgacca tcgctttcag ggcatctgcc
     7621 gctttagtgg aacgtgcttc ctgaataaag ttcagcaacg tagaaatagc gaccattagc
     7681 gcgataacgc ccgcggcaaa taaatcttca gtggcgtaag aaatagcgcc gagaatggtg
     7741 agtaaaatat taaatgggtt gcgatagcag acccataaat gtacccacca cggcgacggt
     7801 tgttgtgcgg gtaatttatt ttcaccatgt tgttcgcagg cagattccac ttccgcctga
     7861 tttaaccctt ccggatgggt gtcgaacgtt ttccataatt cttcttcggg catcaccgcc
     7921 attttcaggc aatgcgcact taaggacggc gggaccgccg tattcactgt ctgctgcgcg
     7981 ccaggcaatg gatcgcgatg aaccagacgg gaaggtaaat ggcgaatgag ccgggtaaaa
     8041 atttctttaa acataaggag tccctccgca ctgtctgaaa cagtacagaa atgtcttcag
     8101 gcgtggcgaa gccttacctg aaggtaataa agctgagcag ggacgttaaa acgtcacggc
     8161 cttacgtatc cggtaacgcc gtgaaaagac gggcttaccg gaaaagtttt aatctccgtc
     8221 gagggagagg cgtgggatca ggttccatat aacctccggt aagtgaaaaa agcgccgcaa
     8281 ataataagga gaatccaata atattcgtag catctatggc gatataatac ctgctggcaa
     8341 ctaaaccaac gataaaccag actttaccca ttgctgaatg cacgggtaaa gttaggctca
     8401 aataattaaa caacacgtta caggacaaca ggatgcaaaa tcggctgacc atcaaagaca
     8461 tcgcacgctt aagcggcgtg gggaaatcta cagtttcccg ggtgctgaat aacgaaagcg
     8521 gcgtgagcca gcgcacccgc gagcgtgttg aagcagtgat gaatcagcat ggattttccc
     8581 cttcccgctc tgcgcgcgct atgcgtgggc aaagcgataa agtggtcgcc atcattgtta
     8641 cccgcctgga ttcgttgtca gaaaatctcg ccgttcaaac catgctgcca gcgttctatg
     8701 aacaaggtta cgacccaatc atgatggaaa gtcagttttc cccgcaatta gttgccgaac
     8761 atttgggggt gctgaaacgg cgtaatatcg acggcgtagt gctgttcggt tttaccggca
     8821 taacagaaga aatgttagcc cactggcagt catcgctggt tctgctggcg cgtgacgcaa
     8881 aaggttttgc ttcggtctgt tatgacgacg aaggggcaat caagatcctg atgcaacggc
     8941 tgtatgacca ggggcatcgt aatatcagtt atctcggcgt gccgcatagt gacgtgacga
     9001 ccgggaagcg acgtcacgaa gcctacctgg cgttctgcaa agcgcataaa ctgcatcccg
     9061 ttgccgctct gccaggcctt gctatgaagc aaggctatga gaacgtagca aaagtgatta
     9121 cgcctgaaac taccgcctta ctgtgcgcaa ccgacacgct ggcacttggc gcaagtaaat
     9181 acctgcaaga gcaacgcatc gacaccttgc aactggcgag cgtcggtaat acgccattaa
     9241 tgaaattcct tcatccggag atcgtcaccg tagatcctgg ttacgccgaa gctggacgcc
     9301 aggcggcctg ccaattgatc gcacaggtaa ccgggcgcag cgaaccgcaa caaatcatca
     9361 tccccgccac cctgtcctga tcgtttcctg aacgataaat tgtgatcttc gctgcgtttc
     9421 gggaacgttc ccgtttttaa atttttccgc gcaatatatt ctgcagccaa ccaaaaatgt
     9481 catctgccat ggggctttat gatgagcaaa ataaaccaaa cggatatcga tcggttgatt
     9541 gaactggtcg gcgggcgcgg caatattgcg acggtgagcc actgtattac tcgcctgcgc
     9601 tttgtcctca accaaccggc caatgccaga ccgaaagaaa ttgagcaact ccccatggtg
     9661 aaaggctgtt tcaccaatgc cgggcaattt caggtggtga ttggcaccaa cgtgggtgat
     9721 tactatcaag cactaatagc gtcaaccgga caggcgcagg ttgataaaga gcaggtaaaa
     9781 aaagccgccc ggcagaatat gaaatggcat gagcagttga tctctcattt cgcggagatc
     9841 ttcttcccgt tgctgcccgc gttgattagc ggcggtttga tcctcggttt tcgcaatgtg
     9901 atcggcgatt tgcccatgag caacggtcag acgctggcgc aaatgtaccc ttccctgcaa
     9961 acgatctacg attttctgtg gttgatcggt gaagcgatct tcttctacct gccggtcggt
    10021 atttgctggt cagcggtgaa aaaaatgggc ggcacgccga tccttggtat cgtgcttggc
    10081 gtgacactgg tttctcccca gctgatgaac gcttatctgc tcgggcagca gctgccggaa
    10141 gtgtgggact ttggcatgtt cagcatcgcc aaagtgggct atcaggcaca ggtgatcccg
    10201 gcactgttag ccgggctggc actgggcgtt attgaaactc gccttaaacg catcgtaccg
    10261 gattacctct atctggtggt ggtgcccgtc tgttcgctga tcctcgcggt gttcctcgcc
    10321 catgcgctga ttggtccgtt tggtcgcatg attggcgatg gcgttgcctt tgcggtacgt
    10381 cacctgatga ccggcagctt tgctccgatt ggtgcggcat tgtttggctt cctgtacgcg
    10441 ccgctggtga tcaccggcgt acaccagacc acccttgcta ttgatttgca gatgattcaa
    10501 agcatgggtg gcacgccagt gtggccgctg attgcgctgt cgaatatcgc tcagggctcc
    10561 gccgtgatag gcattatcat ttccagccgc aagcacaatg aacgcgagat ctccgtgcct
    10621 gccgctatct ccgcctggct tggggtcact gagcctgcaa tgtacggcat caacctgaaa
    10681 tatcgcttcc cgatgctgtg cgcgatgatt ggttctggtc tggcaggatt actatgcggc
    10741 ctgaacggcg ttatggcgaa tggtatcggc gtaggcggcc tgccgggaat tctctcgatt
    10801 caaccgagct actggcaggt atttgcgctg gcaatggtta tcgccatcat catcccgatt
    10861 gtactcacct cgtttatcta tcagcggaaa taccgcctgg gcacgctgga tattgtttaa
    10921 ttttcttcgg ggcgcaattg cgccccctcg catttgcagg aataacgtaa tgactaatct
    10981 tccccactgg tggcaaaacg gcgttatcta ccagatttat ccaaagagtt ttcaggacac
    11041 cacgggtagc ggtaccggcg atttacgtgg cgttatccaa cgcctggact atctgcataa
    11101 actgggcgtt gatgccatct ggctaacccc cttttatgtc tctccccagg tcgataacgg
    11161 ttacgatgta gcgaactata cggcgattga tcccacctac ggcacgctgg acgattttga
    11221 cgaactggtg acgcaggcaa aatcgcgcgg gattcgtatc attctcgata tggtgtttaa
    11281 ccatacctct acccaacatg cctggtttcg cgaggcgctg aacaaagaaa gcccttaccg
    11341 ccagttttat atctggcgcg atggcgaacc agaaacgcca ccgaacaact ggcgttcaaa
    11401 atttggcggt agtgcgtggc gctggcatgc ggaaagcgaa cagtactatt tgcatctctt
    11461 tgcaccagaa caggcggatc tcaactggga gaatccagcg gtacgcgcag agctgaaaaa
    11521 agtctgtgaa ttctgggccg atcgtggggt cgacgggttg cgcctggatg tggtgaatct
    11581 gatctccaaa gacccgcgtt tccctgaaga cctggatggc gacgggcgtc gcttctacac
    11641 cgacgggcca cgagcacacg agtttttgca cgagatgaac cgcgatgtgt ttacgccacg
    11701 cgggttaatg accgtaggtg aaatgtcctc caccagcctt gagcattgcc agcgatacgc
    11761 ggctctgaca ggcagtgaat tgtcgatgac ctttaacttt catcatctga aggtcgatta
    11821 tccgggtggt gaaaaatgga cgctggctaa acctgacttt gtggcgttga aaacattgtt
    11881 ccgccactgg caacaaggaa tgcacaacgt ggcatggaat gccttgttct ggtgtaacca
    11941 cgatcagccg cgcattgttt ctcgctttgg tgatgaaggt gaataccgcg tgcctgcggc
    12001 aaaaatgctg gcgatggtgc tgcacggcat gcagggaacg ccgtatatct accagggcga
    12061 agagattggc atgaccaacc cacatttctc gcgcattact gactatcgcg acgtggagag
    12121 cctcaatatg tttgccgagc tgcgcaacga tgggcgtgat gccgacgagt tattggcaat
    12181 cctcgccagt aaatcccgtg acaacagccg cacgcccatg caatggagca acggcgataa
    12241 tgccgggttt acggctggcg aaccgtggat tggcctgggc gataactatc aacaaatcaa
    12301 cgtagaagcc gcgctggccg atgattcctc ggtgttttac acctaccaaa agttaatcgc
    12361 actgcgtaag caagaagcca tcctgacatg gggcaattac caggatctgc tgccaaacag
    12421 ccctgtattg tggtgctatc gccgtgaatg gaaggggcaa accttgctgg tcattgccaa
    12481 ccttagccgt gagatccaac cctggcagcc agggcaaatg cgcggcaact ggcaacttgt
    12541 gatgcataac tacgaagaag cctcaccaca accctgtgcc atgactttac ggccttttga
    12601 ggctgtctgg tggttacaga agtaaatctt cgctaagccc cggtaatgcc tgtcattccg
    12661 gggttttgcc tgtcagcatg ttgtttttgt tgattttata atcaaaaaat tcattaaaaa
    12721 atttacatat cgctgtagcg cccgtcatcc gtacgctctg ctttttactt tgagctacat
    12781 caaaaaaagt tcaaacatcc ttgatgcaaa gcactatata tagactttaa aatgcgtccc
    12841 aacccaatat gttgtattaa tcgactataa ttgctactac agctccccac gaaaaaggtg
    12901 cggcgttgtg gataagcgga tggcgattgc ggaaagcacc ggaaaacgaa acgaaaaaac
    12961 cggaaaacgc ctttcccaat ttctgtggat aacctgttct taaaaatatg gagcgatcat
    13021 gacaccgcat gtgatgaaac gagacggctg caaagtgccg tttaaatcag agcgcatcaa
    13081 agaagcgatt ctgcgtgcag ctaaagcagc ggaagtcgat gatgccgatt attgcgccac
    13141 tgttgctgcg gttgtcagcg agcagatgca gggccgcaac caggtggata tcaatgagat
    13201 ccagaccgca gttgaaaacc agctgatgtc ggggccatac aaacaactgg ctcgtgccta
    13261 catcgagtac cgtcacgatc gcgacattga acgtgaaaaa cgcggtcgcc tgaaccagga
    13321 gatccgtggt ctggtcgagc agaccaactc ctcgttactc aacgaaaacg ccaacaaaga
    13381 cagcaaggtg attccaaccc agcgcgacct gctggccggg atcgtggcta aacactatgc
    13441 acgccagcac ctgctgccgc gtgacgtggt gcaggcacat gaacgtggtg atattcacta
    13501 tcacgatctc gattactcgc cgttcttccc gatgttcaac tgcatgttga tcgacctgaa
    13561 aggcatgctg acccagggct tcaagatggg gaatgccgag attgaaccgc cgaagtcgat
    13621 ctctacggca accgcggtaa ctgcgcagat tattgcccag gtcgccagcc atatttatgg
    13681 cggcaccacc attaaccgta tcgatgaagt gctggcaccg tttgtcaccg ccagctacaa
    13741 caaacatcgc aagaccgcag aagagtggaa catcccggac gccgaaggct acgctaactc
    13801 tcgtaccatc aaagagtgct acgatgcctt ccagtcgctg gagtacgaag taaacaccct
    13861 gcacaccgcc aacggtcaga cgccgtttgt aacctttggt tttggtctgg gcaccagctg
    13921 ggaatcgcgc ctgattcagg aatctatcct gcgtaatcgt atcgcaggcc tcggtaaaaa
    13981 ccgtaaaact gcggtgttcc cgaaactggt gtttgcgatt cgcgatggcc tgaaccataa
    14041 aaaaggcgat ccgaactacg acatcaaaca gctggcgctg gagtgcgcaa gcaagcgcat
    14101 gtatccggat atcctgaact acgatcaggt agtgaaagtc accggttcgt ttaaaacccc
    14161 gatgggctgc cgcagcttcc tcggcgtgtg ggaaaatgaa aacggcgagc agatccacga
    14221 tggtcgtaac aacctcggcg tgatcagcct gaacctgccg cgtattgctc tggaagcaaa
    14281 aggcgatgaa gccaccttct ggaagctgct ggatgaacgt ctggtgctgg cacgtaaggc
    14341 actgatgacc cgtatcgctc gtctcgaagg cgtgaaagcg cgcgtagccc cgatcctcta
    14401 tatggaaggc gcttgtggcg tgcgtctgaa tgctgacgac gacgtttctg aaatcttcaa
    14461 aaacggtcgt gcgtctattt cgctgggtta catcggcatc cacgaaacca ttaacgcgct
    14521 gttcggcggc gagcatgttt acgacaacga gcagctgcgc gcgaaaggta ttgcgattgt
    14581 tgagcgtctg cgtcaggcgg tggatcagtg gaaagaagaa acgggttatg gtttcagtct
    14641 ctacagcacg ccgagtgaaa acctgtgcga ccgcttctgc cgtctcgata ccgctgagtt
    14701 tggcgtggtg ccgggcgtga ccgataaagg ttactacacc aacagtttcc acctcgatgt
    14761 ggagaagaaa gtgaacccgt acgacaagat cgactttgaa gcgccttacc cgccgctggc
    14821 gaacggtggt ttcatttgct acggcgagta tccaaacatt cagcacaacc tgaaggcgct
    14881 ggaagatgtc tgggattaca gctatcagca tgtaccgtat tacggcacca atacaccgat
    14941 tgatgagtgc tacgagtgtg ggtttaccgg tgagttcgag tgcaccagca aaggcttcac
    15001 ttgcccgaaa tgtggtaacc atgacgcctc ccgtgtgtcg gtaactcgcc gcgtgtgcgg
    15061 atatttaggt agcccggatg cacgtccgtt taacgctggt aagcaggaag aagttaagcg
    15121 ccgcgttaaa catttgggga atgggcagat aggttaatcc ttccccctca ccctaaccct
    15181 ctccctcaag ggagagggta ccgttccgtg ccccttcctg attcccgaca taatgaattg
    15241 cctctggcca gttggtgtcg tcgcaaggtg gccgtagcat cggggaactt cgcccgtacg
    15301 caccagagca gtcatggcgt caattgagaa gcgaaaatga actttcatca gtactatcct
    15361 gtcgacatcg tcaacggccc cggcactcgc tgcaccctgt ttgtctccgg gtgtgttcat
    15421 gaatgccccg gttgctataa caaaagcaca tggcgggtaa attccggtca gccatttacc
    15481 aaagcaatgg aagaccagat cattaacgat ctgaatgaca ctcgtatcaa acgccagggg
    15541 atttccctct ccggcggcga tccgctgcat ccgcaaaacg tgccggatat tctgaaactg
    15601 gtacaacgca tccgcgccga gtgtccgggt aaagacatct gggtgtggac aggctataaa
    15661 ctcgacgaac tcaacgctgc gcaaatgcag gttgttgatt tgattaacgt gctggtcgac
    15721 ggcaaatttg tgcaggattt aaaagaccct tccctgatct ggcgcggcag cagcaatcag
    15781 gtggtgcatc atttgcgatg ataagttccc tgttacctga gtgcaggcaa cagggaaata
    15841 aggaattaac gatacttctg gtgataggcg ttgcgggtcg ttttcagttg ctctgcggca
    15901 gcctgcgctt cttttacttt accttcattt gccagcttca gcgcgtcgtc aatctgaccg
    15961 accagaatat cgaaaccgtg gcggaagtct ttcatttccg ggctgtccgg tgatttgcct
    16021 tcaagcttcg gcggcgtcgc tttttgcgca tccagcgccg cagcgcgcat tttagttaat
    16081 gcgtctttga cttgcaccgc gttatcggct ttttcgacca cttttaaatt gtcgttgagg
    16141 gtttccatat tgtcttccag atcagcggca aacgacgcag aactgaatac caatgaggag
    16201 actgcaagaa tagctaacag gcttttacgc attgctcact tcctttttta tttacacttc
    16261 atccttcaca ctgcctcatc gacggctacg ctcattcgcc cgagtcacgt actacgagta
    16321 cgctccccgg ctctcattca ctttccgtct cgatgcagtg cgaatgattt tgtgtaattt
    16381 tttagtttat agcgcggcag gtcgcgccag tttttttact gcccggcgat tttcatctcc
    16441 ggcaacagca cagaaccaca ctgtatatta ctgcgtgttt caatatcgtt accgacggtg
    16501 acaatattgc gccacatatc tttcaaatta cctgcgatgg tgatttcgct caccggatac
    16561 tgaatttcgc cattctctac ccagaagccc gctgcaccac gggaataatc accggtaatg
    16621 gcactcacgc cctggcccat cagctccgtc accaccagcc cggtgcccat ctctttgagc
    16681 atttgttcga agcttagccc ttgtccggca atccgccagt tgtgaatacc gcccgcatgt
    16741 ccggtgcttt tcagccccag ttttcgcgcc gaatagctgg tcagcagcca ctgagtcagg
    16801 atgccatctt tgacgatatc gcggcgctcg gtgcgcacac cttcgctgtc gaatggtgta
    16861 gatgccagcc ctttcagcag atgcggatgt tcttcaatag tcagccagtc cggcagaatt
    16921 tgtttaccca gcgaatccag caagaaggtc gatttacggt agaccgcacc acccgctatc
    16981 gcccccacca aatgaccaaa caaccctgtt gccacttcat tggcaaaaat caccggcgct
    17041 ttcatggtag aaagtttacg cggtgacagg cgcgataagg tacggcgagc acagtcggcc
    17101 ccaacccact ctggcgtttg tagatcgctc atcgcacgcc caatggtgta ggcataatcg
    17161 cgctccatat cgccattttc ttcggcgatt acacaactgg agagcgaatg acgcgttgag
    17221 cagtaaccct gcaacatgcc gtggctgttg ccaaaaactt tgacaccgta gtggctgtta
    17281 aagctaccgc cttcggtatt ggtgatgcgt ttgtccgcct gcaatgccgc ctgttccgcg
    17341 cgggccgcca gttcaatggc ttcatccgga gaaacgtccg cagggtggaa caagtcaaga
    17401 tccggcgcgt caaaagccag cagttctttg tctgccacgc ctgcatacgg atctggcgaa
    17461 gtgtaacggg caatatccag cgccgcctgt acggtgcggg caatggcctg cgggcttaaa
    17521 tcggtagacg atgcgctacc tttacggttc tggtgataaa cggtgatccc cagcgcgcca
    17581 tcgctattga attcaacatt ctccacttca ccataacgcg tgcttacgct aatgccggtg
    17641 gtcttgctga cggcaacttc tgcaccgtcc gatttgcctg aggccaactc cagcgcagtc
    17701 gaaactgctt cttccagaat cttgcgctgc gcttcaactt gagagattac tttcattgca
    17761 agtgccataa tgtagagagt ttctctgaag tctaacagag aaccgttttt cagtgcgcat
    17821 cttaactggt aacattagcc tcttttttta aggagcctga gatgactaag cagcccgaag
    17881 actggctcga cgacgttccc ggtgatgaca tcgaagacga agacgatgaa attatctggg
    17941 tcagtaaaag tgaaattaaa cgtgatgccg aggagctaaa acgccttggc gcggaaattg
    18001 ttgatctggg gaaaaacgcg ctggataaga tcccgttaga tgcggatctg cgcgcggcta
    18061 ttgagctcgc ccagcgtatt aagatggaag gtcgccgccg ccagttgcag ctcatcggca
    18121 agatgctgcg ccagcgcgac gtagagccta ttcgtcaggc gctggataag ctgaaaaacc
    18181 gccacaacca gcaggtggtg ctgttccata aactggaaaa cctgcgcgat cgtctgatcg
    18241 accagggtga tgatgccatc gctgaagtat tgaatctgtg gcccgatgcc gatcgtcagc
    18301 aactgcgaac tctgatccgc aacgcgaaga aagagaaaga agggaataag ccgccgaaat
    18361 ccgcacgtca gattttccag tatctgcgcg agttagcgga aaacgaaggg taattttcgt
    18421 tgttgatgtg agtatggttt gccggatacg gtgtaaacgc cttatccggc aatgctatat
    18481 caggctgagg ccaaattact gcgcagcttc cgccttcttc gccagcccat ccagcagttt
    18541 ctgatggatc ccaccaaaac cgccgttgct catcaccaga atatggtcgc caggctgagc
    18601 ggttttcacc accatatctg ccagcgtatc cacatcgccg ctccagtgtg caggctgaac
    18661 gcaggcttct gccacttctg ccacctgcca cggaatatgc gccggttgca gcaggaagac
    18721 ttcatcggca cgacctaatg aaggtgccag atcgtctttg cagatcccca ttttcatggt
    18781 attcgaacgc ggctccagca cggcaataat gcgcgccgta ccgccaactt tgccacgcag
    18841 cgccgccagc gttgccagaa tcgccgtcgg gtgatgggca aaatcgtcat ataccgtgac
    18901 gccattcgct tcaccacgca actccaggcg gcgacgagcg ttaataaacg aacccagcgc
    18961 gttagcggca tctgccggcg ctacaccaac atggcgagcc gccgcaatcg ccatcaggcc
    19021 attgtgcata ttatgttcgc ctaccagcga ccatttcact tcgcccactt tttcgccatc
    19081 cagcaagact tcccattcag aagcgtcagt ggtcagcttt ttcgcctgcc agtgcccctg
    19141 ctcgcccacc agctcctgct cgctccaaca gcccatcgcc atggtctgtt tcaggttgat
    19201 gtcgttttcc ggccagataa tacggccctg ccccggaacg atacgcacca gatggtggaa
    19261 ctgtttctgg atcgctttca ggtcgtcaaa gatatcggcg tgatcgaact caaggttgtt
    19321 gaggatcagc gtacgcgggc agtaatggac aaatttagag cgtttgtcga agaaggcgca
    19381 gtcgtactca tccgcttcga taacaaagaa atcgctttcg cccagacgcg ccgagacctc
    19441 aaagttcccc ggcacaccgc cgatcacaaa tcctggtttg taaccacact gttccagaat
    19501 ccaggtcgcc attcccgccg tggtggtttt gccgtgtgta ccggcaacgg ccagcaccca
    19561 gcggtcacgc agcacaaaat cgtgcagcca ctgcggacct gacatataag ggatgttttt
    19621 ttccagtacc gcttccacac acggatttcc acgggtcatg gcgttgccaa taatcaccag
    19681 atccggctgc gggtcgagct ggctggcatc gtaaccctga atcagttcaa tgccttgctt
    19741 ctcaagtaag gtgctcatcg gcggatacac attggcgtcc gaacccgtta cttcatggcc
    19801 taactggcgc gccagcatcg ccagaccgcc cataaacgtg ccacaaattc ctaaaatatg
    19861 aatgcgcata cgtcactatc cttttttaat ctgccgctca ttttacgcat atgtcgggca
    19921 agtgagaaac gcatttcagg ataatccgca atttgctggc gcgattcacc tgagcgcaac
    19981 attgagatta tttgttaaga ttgttgcggt cgctttactc cataaacatt gcagggaaag
    20041 ttttatgaaa acgttaggtg aatttattgt cgaaaagcag cacgagtttt ctcatgctac
    20101 cggtgagctc actgctttgc tgtcggcaat aaaactgggc gccaagatta tccatcgcga
    20161 tatcaacaaa gcaggactgg ttgatatcct gggtgccagc ggtgctgaga acgtgcaggg
    20221 cgaggttcag cagaaactcg acttgttcgc taatgaaaaa ctgaaagccg cactgaaagc
    20281 acgcgatatc gttgcgggca ttgcctctga agaagaagat gagattgtcg tctttgaagg
    20341 ctgtgaacac gcaaaatacg tggtgctgat ggaccccctg gatggctcgt ccaacatcga
    20401 tgttaacgtc tctgtcggta ccattttctc catctaccgc cgcgttacgc ctgttggcac
    20461 gccggtaacg gaagaagatt tcctccagcc tggtaacaaa caggttgcgg caggttacgt
    20521 ggtatatggc tcttctacca tgctggttta caccaccgga tgcggtgttc acgcctttac
    20581 ttacgatcct tcgctcggcg ttttctgcct gtgccaggaa cggatgcgct tcccggagaa
    20641 aggcaaaacc tactccatca acgaaggaaa ctacattaag tttccgaacg gggtgaagaa
    20701 gtacattaaa ttctgccagg aagaagataa atccaccaac cgcccgtata cctcacgtta
    20761 tatcggttca ctggtcgcgg atttccaccg taacctgctg aaaggcggta tttatctcta
    20821 cccaagcacc gccagccacc cggacggtaa actacgttta ctgtatgagt gcaacccgat
    20881 ggcattcctg gcggaacaag cgggcggtaa agcgagcgat ggcaaagagc gtattctgga
    20941 tattatcccg gaaaccctgc accagcgccg ttcgttcttt gtcggcaacg accatatggt
    21001 tgaagatgtc gaacgcttta tccgtgagtt cccggacgcg taatctcctg cgcctctcca
    21061 cacggagagg cgtttttatc gctgcgcaat gttgactctt gtcacaggcg aactctgacg
    21121 attctcccac agcaccgtta atccacgctg taaggcaata aaaatgaaca gcaaaatacc
    21181 gatggcgatt ttcgtccacc aggaactcag cgtgccatca aagtttatgt aagtctgaat
    21241 cagtccctga atcgccacgc caaaaagcgt ccctaatacc gttccaacgc caccgctcaa
    21301 aagcgtaccg ccaattacca ctgaggcgat agcatccagt tccacaccta cgcccgccag
    21361 cgcatatccg gcctgggtat aaatcgagaa gacaatcccc gccagcgttg ccagtccggt
    21421 ggagagcata taaatgcgaa tagtggtgct gcgagtggaa atccccatca ggttcgccga
    21481 cgttgcgttg ccgccaatgg cgtatacctg attaccaaaa cgggtacgat gcgcgaggaa
    21541 aataccgata accaccaccg ccagcatcag cagccccatc gcacttaagc gaccgccgcc
    21601 agggattttc catgcaaggc ttgagagcgt gtcataaaca ggatgattta tcgggatcga
    21661 ctcttccgaa acgagatagc tgacgccacg caaaaagaac attcccgcca gagtaataat
    21721 aaacgccggg atcttcaggg cgtcgatcaa aagccccata aatgcgccga aagcacagcc
    21781 catcaccagc accagcggaa acgccagcaa cggcgagagg ccgaaatcgc caatcacttt
    21841 tgccagaaac acgccggtaa aggcgatcac cgaaccgacg gagagatcga tcccaccgga
    21901 gaggatcaca aaggtcatac caacggcgat tatcccaaga aaggcattat cagtcaggat
    21961 attgcagatc actcttgtgg aagcaaaacc gggaaactgg gtcagacagt agatataacc
    22021 caacacaaag acgccgatgg tgatcatcag tggcaaatta cgttttatca cggttacgtc
    22081 cccctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
    22141 ccgcttttac gacctggttc atctccggcg gaaagccaga aagcaaaatt ccggtgttca
    22201 ttccctgaat aatcagcgcc cccaccaccg aaagcagcag gttaaagcgc ccgcccatca
    22261 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aacccggcgt
    22321 tattggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
    22381 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
    22441 ccgcccgaat attgatacca acggcttcga taaacatccc cagtgccgtt ttgcgggtca
    22501 acagccagaa caggagaagc gtcagcaccg caataatgac tggcgttggc aggaacaaca
    22561 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggagg
    22621 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
    22681 gaattttaag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccagtgccca
    22741 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
    22801 ctccggcgat agccatcact gcccctacgg agagatcgat accacctgta gcgatcacca
    22861 gcgtcatgcc aatcgccagt aacgcaacgg gtgctgcacg gttaagaatg tcgatagggc
    22921 taccgaacaa acgcccatcc tgaagcacaa cctgccagaa atgcggggcc accaggctgt
    22981 cgaccagcag caccagcaat agcgctacca gttgcggcat ccccgtcggc cagcgaaagc
    23041 gccttttcga cggcgtagtg tccgggagag attgaggcat cacactgttc tccttacgcc
    23101 gcaatggcgt tcatgatcgc cggaacggaa agctctgcca gcgggatctc tgccacctgt
    23161 ttgcgatcgc gcatgataat cacccggtcg gcatagccca ccagttcttc cagttcggag
    23221 gagatcacca gcagcgccag accatcagcg cacagcgttt caatcaggcg gatgatctcg
    23281 gcgtgcgcac caacatcaat gccgcgggtt ggctcgtcga ggatcaaaaa ttgcggacga
    23341 gtcagtagcc aacgtgaaag caacactttt tgctgattgc cgccggagag aaattcaatc
    23401 ggttgttcgg tggaaggtgt gcgaatgcca agctggcgga taaagcgttg ggcaatctct
    23461 tgctgttctt tgcgggaaat cggtcgtagc cagccgcgct gggcctggag agcaaggatg
    23521 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
    23581 aagccgatac ccagtaccga agcctgatgt ggcgatcgca gggtttgctg tttgcctttg
    23641 atcaacgccg tgccgctgtc agcaggtttg atcccgaaga tcacttcggc ggtttcggta
    23701 cgtcctgatc ccagcaagcc tgccaggcca acgatctcgc cggggcgtac ttcgagatca
    23761 aacggtgcga tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
    23821 agcaatgttc gtccggcacg ctgtagcgcg tgggtgtcca gctcgcgccc cagcatcatt
    23881 tttaccagtt cgatctgcgg tagctcgcgg gtttcccgac agcctacgaa actgccgttg
    23941 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtaacgaag
    24001 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaacagtaa ctctacttcc
    24061 tgagtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgatg
    24121 gcccggcaaa tggcgacgat ttgctgcatc gcgactgaaa agcggttgag cggttcgcgc
    24181 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
    24241 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcgaccgac
    24301 atattaggta gcaggttgac ttcctgatag acggtgccga tgccgagttg ttgtgcatgg
    24361 gcagtgtttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
    24421 tatacaccgg ttaaggcttt gattagcgtt gattttcccg ccccgttttc accgagcagc
    24481 gccataattt cgccgcgacg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
    24541 aaaaatttac ttaatccttc ggtgcggagg atttcctggt gttggttagt attcatagtt
    24601 tcccccggtg taaagcgtgc gcccggtaag ccagataaga cacaacagcg ttgcatcagg
    24661 catctcgcac aaccgccgga tgcggcgcga gcgccttatc cggcctacga atcggcatca
    24721 ggcatagcaa ccctcaatac cccatatttt tcttcttctc taactcttct tttgcggtat
    24781 caggcagata aagggtggat ttggtcaggg tcagcttttc aggcatggtg ccgtcttttt
    24841 tgtatttctc cagcgcatca aaagcggggc ctgccatgtt cggcgtcagt tcaacgctgg
    24901 cgttcgcttc gccatcaatc atcgctttgt agatgtccgg cacgccgtcg atagagcctg
    24961 tcaggatatc tttgcccggt ttcaggcccg cttctttaat tgcctgaatt gcaccaatca
    25021 ccatgtcatc gttatgggcg taaaccatgc agatgttttt gccgttgttt tccgctttga
    25081 taaagctctc catcacttct ttgcctttgc tgcgggtgaa gtcacctgac tgcgagcgga
    25141 taattttgat atttggcgca ttcttaatgg cttcggcaaa gcctttctta cggtcaatgg
    25201 cgacgctggc tccaacggtg ccctgcaact ccaccacgtt acacggtttg ccattcactt
    25261 cttttaccag ccagtcaccg atcaacttac cttcgaggat gttgtcggca gtcacggtgg
    25321 tcatatagag agatttgtct ttcacatcga tggaacggtc aagcaagaag accgggattt
    25381 cggcatcttt cgcctctttt aataccggct cccaaccggt cgctaccact ggagcgataa
    25441 agatcgcatc caccccttgc gcgacgaagg aacgtaccgc tttaatctgg ttttcctgct
    25501 tttgctgacc atcggcaatt ttcaacgtaa ttccgcgctt ttcggcttca cttttcgcca
    25561 cattggtttc tgcggcgcgc cagccggatt ccgatccgac ctgcgaaaat ccaacggtta
    25621 atggagcggc caacgccata gacgacatgg ctgccgagac tgcagagact acaagtaagc
    25681 gtttccacat agggttgtcc tcgtcggggt aattattatt ggtgaaaaga tgttcgcgaa
    25741 aaaactatag acaattcgtt atgtaacgga ttgcgttaca tcacacttca aaaaagtaaa
    25801 taaaacgtta atcacaagtt tgtaatcgct ttcatctcac tatgaaaaat gcggctacgg
    25861 ttatggattt tcctgctctg tataccgtct taaaactggc gaaaaaggaa aatgaagacg
    25921 aaaacaagcg aagacattcg gcgcgagttg gctataatac tcggcacttg tttgccacat
    25981 atttttaaag gaaacagaca tgagcttact caacgtccct gcgggtaaag atctgccgga
    26041 agacatctac gttgttattg agatcccggc taacgcagat ccgatcaaat acgaaatcga
    26101 caaagagagc ggcgcactgt tcgttgaccg cttcatgtcc accgcgatgt tctatccgtg
    26161 caactacggt tacatcaacc acaccctgtc tctggacggt gacccggttg acgtactggt
    26221 cccgactccg tacccgctgc agccgggttc tgtgatccgc tgccgtccgg ttggcgtact
    26281 gaaaatgacc gacgaagccg gtgaagatgc gaaactgatt gctgttccgc acaccaagct
    26341 gagcaaagaa tacgatcaca ttaaagacgt taacgatctg ccagagctgc tgaaagcaca
    26401 gatcgctcac ttcttcgagc actacaaaga cctcgaaaaa ggcaagtggg tgaaagttga
    26461 aggctgggaa aacgcagaag ccgctaaagc tgaaatcgtt gcctccttcg agcgcgcaaa
    26521 gaataaataa gttcttctag cgtaataacc ctgaacgccg ggcttcggtt agtaggggtt
    26581 ttttatgccc gcgataaata aacacacctt attccaccac cgcctgcaaa cgtaataacg
    26641 cctcttccac cacctcaccc gcagccagac caatacgctt tgccagtcgg gcgcgtagat
    26701 ccatcatccg cacctgattc accagcacca cgccgtgcac atcgccttct tcgcaatgta
    26761 aaggaacgct aaatccggca taacgggcaa aatttccgcc ctgcgtaatg ggggccacca
    26821 gcgtcattcc cagttgatta aaggcttgaa cggagagcac aagcgcaggt cgaccagcac
    26881 cttgctgttc atggccgctt gctggatcaa agccaaccag cacaatgtct ccccgttcaa
    26941 attcactttt ctttaccata tttcgtcacc cgcaggggtg gatttacccc agacatcctg
    27001 ctcgctaagt tccgtggcgt tcatgtcaca ctgtgccagc agttcatcaa gcgagtagcg
    27061 cctggagatg ggtgtcagaa tcagttgatt gttgctcact tgcgcctcca cgctctgccc
    27121 cggccgtaag ttaagttctt tcatcacaac attaggaatg accatacctg aactgttccc
    27181 ccatcttttt atggtaatac gcatctgcac ctccttaagt tatacaaagt ataactcacg
    27241 gtgctacagg aaacgtttta cccctgcgca tttgtgaacg ccagcagagt tttttatgcg
    27301 ttaacagata tggaaagcgg ctcggagaga aaagcaaaag gtgagggaat tacaaaacag
    27361 aatgcgagtc gtctcgcaaa aaacaacgcc acccgaaggt ggcgtatgcg tatggttact
    27421 tatccctgtc taaccagtcg ccgctttcaa ttagctttaa tccatcgacg ggtcgttgat
    27481 aaacgtacat ccatgcactc ccgtacggcg tctgaatcaa ctggcgcgcg tattcaccgc
    27541 ccctggtgcg caaggcatca agttcggcca gcgtggcgtt gtcaatacga taaacttcac
    27601 cgtgtactgt tccgttcccc ggaactgcgc ctggatagtg gcccaggcta tacaactggt
    27661 agttatcgat actgaaatcg cccagtaact gggcattggt catccagtga ctgttgcctt
    27721 gtttgtggcg taaactgccg tagacaaata ttcgcattgc taaaactcga actgatagag
    27781 caaatccagt gcctggtcta caccagacac ggcttccaga tatagcttag gcatcaggcg
    27841 ataacgtaac gtgagtgttg ctatagagtc aaatataccc acgccgtatt tcacttgcag
    27901 acctggcaat acatagccgc tgaccaccac ctgggaggag tcgcctactc cctgggtgtc
    27961 gagcgctaaa ttgcttacgc caaacgtctc gccgatttta cccacaatct gaccactttg
    28021 tgcacccccc agaccaatca gcattgaggt catcgccgca ctgtcgctct gatcgctctc
    28081 cagcccttgt ccacgtagca aataagacaa tgcagcttgt tgcgacatcg ccgggtcaga
    28141 gaagatctcc gctttcggtt cgtccgccag accagtgacg cgaactccgg cgatgacgtc
    28201 gtcttctgta gcatccgggt tacgaatagc ttcaatatta agatacggtt gatccggcgg
    28261 gccagagaac agtagctcac ccttacgcac aatcagatcc tgaccatagg catggaagcg
    28321 cccttcaggg atgttgatct gcccgttcag gcccagccct tgtttgtcct gaacgacgtt
    28381 aagatcgccc gtcagccgcg ctttcaggcc aaaggcgtca atgcgcacat tgttgccgac
    28441 gtgaacaatc aggttgctgt taatcggaat cgacgccgtt tttgcctctt ccggttgcag
    28501 gttatcgtta agcatcacca catcgctgga gacgcccacc gcgctttccg gcagctcatg
    28561 caccacgatg cgcgcccacg ggacatccac gcgaccatcg agggtaaaca ggtttggtgt
    28621 agcctcgaat acaacatctg gtgatacatc cattcgtacc atcggcggca cggtgatacg
    28681 cactttactg cccttcgctg ttacccgcgc ccgccagttt tcaatctggc tccagtcggc
    28741 gtcgccgttc aggtagatct caccctgctg ggtccgtact gtaccggcaa gcgtcgagcg
    28801 cataccgtta aagttgaccg caagctggct cggctgcata tcaaacggca taaagttgcc
    28861 gtcgatatcc acacccgtaa cctgtagcag cccaaacaac tgcgggcttt gcacatcacc
    28921 acccagacgt aagttggcac tcaccatccc cgcagctttt tccccacggg taaagatagg
    28981 gtttatcatc gcaaggttga agttacggat attgacgttg ccaccaagat tacggcggcc
    29041 ttgcggatcg gtcacctgca cctgtccatc aaactggccg ttattggtca ggcggatggt
    29101 ccagcccaat tcggcacggt tgttacgcaa ttccgccgtc agattcagcg tctgaaacgc
    29161 cacgggcagc gccgcatcgt tgacggtttg cgttacctgc acattacgcc ccgaaagggt
    29221 gatactgccc tgcggcagcc cctctttcgt ggtgtcccag gcaacgtccg ctttacccgt
    29281 gaagataccg ctggcctgag tggtttctgg cataaacggt ttcagcatgg cgaggtcgaa
    29341 gcggttgaga ttcaccaccg cacgcccttc ggcccccgca tcgatagttt gcggcacgca
    29401 caattccgca ttcgggttaa gccaacagtg tggccagatg ctgatttttt gctccttatt
    29461 gcggtaatcc agcgcaatat cgcgggtcag cgaccacggg ccgaccggcg tctggaagcg
    29521 ggtattgcta agagttccct tccagcgttc ttctttgcga tcaaaacttc ctgccagatt
    29581 aagctgcccg gaaaccggct cgccctgaat ccgcaactgt aactcgtgct gttgttcgct
    29641 gcctttggca ttcagggtga cgaggttgat actcacatcg ggctgcgaga tttgctcaac
    29701 gcgtacgtca agtttacccg caatctgatc ggtggatttg atatcgcctt ccacgcgaac
    29761 ctgcgccacg gaaagttcct gccagcgcag gccgcgcgcg gtgatatctg ccagtagttg
    29821 tggcgcttcc accgtgccgc gtactttcac cagccctttc gctgtaccgc caagccccgg
    29881 cagcgcgtta tccagccccg gcgcgttgat ggtggcatca agattgagat cttttacccc
    29941 cagctcgcct ttcacttcgg cactgtttgg ccccagttcc agatgcagcc ctgggatcat
    30001 ccactgcata taactgttgc ctttcagcgt gccgtcaacg ttcactttgt tctgtttgac
    30061 gttaccggtc agcttcagtt ctggcacttc catctgccag gtgccgccgt acaggctacc
    30121 gcgggtttta atcaagccat taagtttcga cggccactcc gggaactctt tggcggtgtt
    30181 aatgccgtta agcgttagct caccgcgcca gctaattgcc tgctgccagt cgagcaacgc
    30241 tttgagttca gttttccctt ccagcgccgc gacggtgagt ttgtcgagat tgacctgctg
    30301 ttcattacct ttggcatcaa gggtaatggt ggctggcggg atctcctgtc ccttcactgc
    30361 cgtacgcata gagagcgtgt aatcggtcat tttaccggta agtttcagtt tcagatcatc
    30421 cgcctgatac tgcttctctc cagtgaacgg ccagtaaagc tgtttgctgt tcacttccac
    30481 gttgagcggc aatccggctt ccgccagtcg cgtctgggcg cgtaaatcca tatccaccgg
    30541 accagaaagg ttaacgccta tctccagctg ttcgcgcagc gcgccgccca ctttcagctt
    30601 cactttttca cctttcaacg gctccacgtt cagtgtgctg ttgagggtaa tatccaccgg
    30661 ccagttgtct gacagctgcg ccgtgccgct ggcgttgacg atcccttgac tggagtcgat
    30721 atccagggcg tccagtttag tattaccgtc gatgctgctc actttcagca gcatggtgct
    30781 cacggtgatg tccgtgtcac ccgtcacgcg cagctgctcg cccttaaact cttcaatgtt
    30841 caggttaagc ggcaaatgca cgtcggtcat ttccggcaat accgggcgag aaaagagatc
    30901 tttcagcgtt tcgccgagcg gtttttcatc cggctgcgga ttttcaattt tcggttcgac
    30961 aacttcttcc tgcgccactt ccgccacttt cggcaaagca atcaacaggc ctttcagcga
    31021 cgtcggtttc agggtcaggg ttttctcctg ccagttcagg ccggaggtga agtccatcac
    31081 cgacaccgtg gtgtcatcaa tcttgatgtt gacgttgtcc agcgccaccc gtgtcagggt
    31141 gatgggatac ggcgtggaga gatccagcgg gccgctatct tcctcttctt caacctgttc
    31201 agaaggaggc atttttttac tgtcgatgtt gacctgaatg tctttcagcg ccagatcatt
    31261 aatacaaacg ctactgttcc acaggcactc aagcccgacc gccagatgca gattgcccgc
    31321 ttttaccgct acgcctggct gctcataacg aacgtcagac aaggtgagat cacgccagcc
    31381 gccggtgacc ttgccaatat ccagtcctgg cacccagcga tccgccgctt taaataccag
    31441 atgcaggccg ctggtggtgc ccaccagaaa cgccaccgat cccagcaaca gtaagataac
    31501 gataaccacg ccgaggctga tttttttcca taaactcata attctggccc cagaccgatg
    31561 taaaactgta acccgtgttc gtctttatcc gcgaccggta cggcaaaatc gagtttgatt
    31621 ggcccgaccg gcgattccca gcgcacgccg accccggtac cggttttaaa gtcgctgcgg
    31681 cgaatatcgc ttaccgcttc gccactatcg acaaacaccg cgccccacca ttttccggtc
    31741 acgttgtact ggtactccag cgatccggtt atcaacttcg aggccccttt caggtcaccg
    31801 ttggcgtatt tcggagcgat tgatttgtat ttgtagccgc gaatactgcg atcgcccccg
    31861 gcgaagaaac gcagatccgg cggtactttg tcgaaatcac ccgtttcaat ccagcccagc
    31921 gtgccgcgtg tcacaaaacg atggcgatcg tacagtgtgc ggatccagac gttctgcgcc
    31981 tggaaaacgg agaaatcgac atctgagccc caggccgtgt tggagtagtc gatagagtag
    32041 cgttgcgagt cgccccaggt tggcatcagg ccaccgcgag aacgcgtgcg gctaatcatc
    32101 accccaggat aaaacagcat cgtggtgttg gtaatttcac cctgggtaaa gtggtcgaga
    32161 ctccagcgca ggttaatggc acgctgccag ccgctggaga gatcccagta gcgagaagcc
    32221 accagcgtag tggagtcaga ctcggtatcg ttcaggtcag tgcgcttaaa accgccctgc
    32281 accagataat attgttccag tggattcttc aacagcggca ttttatagct gaagtcgagg
    32341 atctgttccg gcgcggaaat actggtactg gtggtcagac tgtgaccata agagttcatc
    32401 cacggctttt tccacgtcgc tttcacgcgc ggtcccacgt ccgtagagta accgaccccg
    32461 gtttcgatgg tgttttcagt tcgcggcgaa accacgcccg tcaatggtaa tactttcgtt
    32521 tcgcgcgatt tatcaaattg tggagccact acgaccgagt taaaccagcc ggtagcagaa
    32581 agtcgacggt tcagctctgc cagatctttc gattcgtact catcgccctc tttaaacggc
    32641 accagatttt gcaggtattc atcgcggatt tgtgatcctt caaaggtcac atgcccaaag
    32701 cggtaacgtt cgccactgtt ataatcaata tcccagaagg ctttatgcag gccgagcgca
    32761 atgcccagct gcgctttggt aaattcgcta tcgaaataac ctttacgcaa cgcaatgctg
    32821 gttaaggact ttttgaaatt ttcataatcg ccctggttca gtaccgtacc aatagccggg
    32881 cgagtatcga gcaatttcaa atagtcttta tcggtccgcg cgccgccgcg caataccaca
    32941 tcggtgccgc caattaacac cggcactcct ggcgtgactt tggcgatcaa tacctgccgc
    33001 cctttctttg gcggtggacg gagatcaaat tcaatggtcg gctggtaata acccagcgct
    33061 ttcagacctt cgcggatggc atcatcgacg cgtgcgcgaa agcgacggtc tggcgtcact
    33121 tcatcacttt caatcgtaga aagctgcgca cgaacgttct tttccagctg tcccgataac
    33181 ccctcgacct gtagacggac gttcgcggcg acggcagatc cgcttaagca gagtaagctt
    33241 acacagcata actgtcggat atagcgcaca ttttctcctg aatatccttt tttcctgccc
    33301 ctggaacgcc gttaaacggc ttaacaaaaa tccagtaata tggattaaaa agcagactaa
    33361 accccaaata tttcttatgt tttactttag acctattcac agcggttatt gtgtgcaaat
    33421 acgcctcttg ttacaacctt aaccccaatg accgattttc gggagagcga caccatgagt
    33481 ttatttgata aaaagcatct ggtttccccc gccgatgccc tgcctggacg taacaccccg
    33541 atgcccgtag ccacgctgca tgcggtcaac ggtcactcaa tgaccaatgt acctgacgga
    33601 atggagattg ccatttttgc gatgggttgt ttctggggtg tggagcgtct gttctggcag
    33661 ttacccggcg tttacagcac cgccgcaggc tataccggcg gctatacgcc aaatccgact
    33721 tatcgggaag tgtgctccgg cgatactggc catgccgaag cggtgcgcat tgtttacgat
    33781 ccatccgtca tcagctatga gcagttgcta caagtatttt gggagaatca cgatcccgcc
    33841 cagggaatgc gtcagggcaa tgaccacggt acgcagtatc gttcagcgat ttatcctctg
    33901 accccagaac aggatgccgc cgctcgcgcc agtctggaac gttttcaggc ggcgatgctt
    33961 gccgccgatg acgatcgtcg catcaccacg gaaatcgcta acgccacacc gttttattat
    34021 gccgaagatg accaccagca atatctgcat aaaaacccgt atggttactg tggaattggc
    34081 ggaattggcg tctgtctgcc gccggaagca tagcgttacg ggtacaaatg tagattgttg
    34141 ataaagtgct atttgtctat gccggatgcg gcgtaaacgc cttaccaggc ctacaaaatc
    34201 gtccaaaatc aataaattgt ggggactgcg taggcctgtt cggcatagcg catcagccag
    34261 tttttcattt ttcagctgtc tgaattttaa ccctctggcg gctttacagt accttacgct
    34321 atactagcca ctgaaaatgc cggttcactt tcttcgaatc ggctttcaat gtgtatttca
    34381 cacaaattaa tcaacttccc cttccgagga tctggcctga aaggtcggat aagatatgtt
    34441 aaacagtatt ttagtcatac tctgtttgat cgctgtaagt gcgttcttct cgatgtccga
    34501 gatctcgctt gccgcctcac gcaaaatcaa acttaaactg ctggctgatg aaggcaatat
    34561 aaatgcccaa cgcgttctga atatgcagga aaatcccggc atgttcttta ccgtggtcca
    34621 aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc ggtgatgcgg cattttctcc
    34681 agcttttcac agcctgttct cccgctatat gtcggcagaa ctctctgagc aactgagctt
    34741 tattctctct ttctcgttag tgactggcat gtttatcctg tttgcggatt taaccccgaa
    34801 acgcatcggt atgattgcgc cagaagctgt ggctttgcgt atcatcaacc cgatgcgctt
    34861 ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac ggactggcga acataatctt
    34921 ccgtattttc aaactgccaa tggtacgtaa agatgacatc acttctgatg acatctacgc
    34981 ggtagtggaa gccggtgcgc tggcaggcgt gttacgtaaa caggaacacg agctgattga
    35041 aaacgtcttt gagctggaat cccgtaccgt tccgtcttca atgacaccgc gtgaaaacgt
    35101 gatttggttt gatctccacg aagatgagca aagtctgaag aataaggtgg cggaacatcc
    35161 gcactctaag tttctcgtct gtaatgaaga tattgaccac atcatcggat atgtcgattc
    35221 taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg gcactgaaca gcggcgtaca
    35281 aattcgcaac acgctgattg tgccggatac gttaaccctt tcagaagcgt tggaaagttt
    35341 taaaaccgca ggtgaagact tcgcggtgat catgaacgag tacgcgctgg tagtggggat
    35401 catcaccctc aacgacgtga tgaccacgct gatgggcgat ctggtcggtc aggggctgga
    35461 agagcagatt gtcgcccgtg atgagaactc atggctgatt gacggcggca ccccgattga
    35521 cgacgtcatg cgcgtgctgg atattgacga gttcccgcag tcgggcaact acgaaaccat
    35581 cggcggcttt atgatgttta tgctgcgtaa gatcccgaaa cgcaccgatt cggtgaaatt
    35641 cgccggctac aaatttgaag tggtggatat cgataactac cgtatcgacc aactgctggt
    35701 gacccggatc gacagcaagg ccaccgccct ttcgccaaaa ctgcctgacg ctaaagataa
    35761 agaagaaagc gtcgcgtaac ccccagaaac atcaacggct cctgaatcag gagccgtctt
    35821 attactgcat agcactttgg ttaagccatc tctgtttgca gacgcataac ctgacggtta
    35881 acttcggaca tcactgacaa atgcagctta tccttcactt ttgggataag aatcttaccc
    35941 ttatcaaatt caaaagcgcc aacgtccttg atgtataacc gtccacggaa caggatcttt
    36001 acgtacttcg ccacctgaag tgggttgtac cgttggaaaa ttttcattgt tatctcctgc
    36061 tgagtattac gcctgtgcgg tgccacaatc ggcccaacta ttatgaggcg caaattttaa
    36121 tgcctagtga ctatagacta tccgggcaat gtttccaccg cgtataactt tttttaccta
    36181 aaggttacaa ttattcagaa ttatcttttt accgaagcgc ggtcttcagt ataagcattc
    36241 atttttcata tgatttgtgc gcttgaccgc aaactggcat cacacttgcg ggaaattcga
    36301 taaatagcac atatgattaa aactcagacc caagtggtcg gatcacctgc atatcataag
    36361 aaggaaacac catgacccta cgcaagattc tggcactcac ctgcctgctg ttgccgatga
    36421 tggcttccgc acatcagttc gaaaccggtc agcgagtgcc gccgattggc atcaccgatc
    36481 gtggcgagtt ggtgcttgat aaagatcagt ttagctacaa aacctggaac agcgcgcagt
    36541 tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg cacctctgca aaagagaaaa
    36601 acgcgacgct gattgaagcg attaaatcag cgaagttacc gcacgatcgt taccagacca
    36661 ccaccattgt taacaccgac gacgcaattc cgggttccgg catgtttgtg cgcagcagtc
    36721 tggagagtaa taaaaagctt tatccctggt cgcagtttat tgttgatagc aatggcgtcg
    36781 cacgcggtgc ctggcagctg gatgaaaaaa gttccgctgt agtggtgctg gataaagacg
    36841 gtcgcgtgca atgggccaaa gacggggcgc ttactcagga agaggtgcag caagtgatgg
    36901 acctgctgca taaattaatt aataaataga cactctgaac cccggattca ggaacgactc
    36961 gcgcggagtg taatccagcg gtttaccctg ccagtcgtga acgtgcgctc cggcagccgc
    37021 agccacagca tgaccagcgg cggtatccca aatattcgtt ggcccgaaac gcgggtacag
    37081 ctgcgcctgt ccttccgcca ccaggcagaa tttcagcgaa gagccgatgg acgtggtctg
    37141 atgttcgcca agctgttgca gatactcttt cagctccgca tctgcatggg aacggctgat
    37201 caccaccagc ggcgggcgcg catcacggac ctgaatctgc ttgcgcacgc cgcactcttc
    37261 tttccacgct ttgccttctg ccgcgctgta cattacgttc attaccggcg catacaccac
    37321 acctaaaatc ggtttgccgt ggtcgatgag cgcaatgtta acggtaaatt cgccattacg
    37381 tttaataaac tctttagtac catccagcgg gtctaccagc cagtaacgct gccagtgctg
    37441 acggacttcc caaccgggag gatcttcttc agaaaggacc ggaatatccg gtgtcagcgt
    37501 acgtaaaccg tccatgataa cggtgtgagc ggcaatatcc gctgccgtta ccggagaatt
    37561 gtccgctttg ctgacgacgt ccatcggttt cgtcccgtcg tagacctgca taatggcatc
    37621 gcctgcattc cgtgcaagct ggcatacttg atctaacatt tctccacctc gtctctgtga
    37681 gcggtgttaa cttattgttt tacttatacc ctatcgttaa tgaatgcgcc aactgtgata
    37741 gtgtcatcat tttcaaagcg taaaattgtg gcattcttca ctgttctata agtaagacgt
    37801 ttattctccc ttttctttcg tattcccgat gataaaagga tgtccctgat gattaagttt
    37861 agcgcaacgc tcctggccac gctgattgcc gccagtgtga atgcagcgac ggtcgatctg
    37921 cgtatcatgg aaaccactga tctgcatagc aacatgatgg atttcgatta ttacaaagac
    37981 accgccacgg aaaaattcgg actggtacgt acggcaagcc tgattaacga tgcccgcaat
    38041 gaagtgaaaa acagcgtact ggtcgataac ggcgatttga ttcaggggag tccgctggca
    38101 gattacatgt cggcgaaagg attaaaagca ggtgatattc acccagtgta taaggcatta
    38161 aatacgctgg actataccgt cggcacgctt ggcaaccatg agtttaacta cggtctggat
    38221 tacctgaaaa atgcgctggt gggagcgaaa ttcccttatg taaatgccaa cgtcattgac
    38281 gccagaacca aacagccaat gtttacaccg tatttaatta aagacaccga agtggtcgat
    38341 aaagacggaa aaaaacagac gctgaagatt ggctatattg gcgtcgtgcc gccgcaaatc
    38401 atgggctggg ataaagctaa tttatccggg aaagtgacgg tgaatgatat taccgaaacc
    38461 gtgcgcaaat acgtgcctga aatgcgcgag aaaggtgccg atgttgttgt cgttctggcg
    38521 cattccgggc tgtctgccga tccgtataaa gtgatggcgg aaaactcagt ttattacctc
    38581 agtgaaattc cgggcgttaa cgccattatg tttggccatg cccacgccgt tttcccaggt
    38641 aaagattttg ctgatatcga aggagctgat atcgccaaag gcacgctgaa tggtgttccg
    38701 gcggtaatgc cgggcatgtg gggcgatcat cttggtgtgg tcgacttgca actcagtaat
    38761 gacagcggta aatggcaggt aacacaggcg aaagcggaag cacgaccgat ttacgacatc
    38821 gctaataaaa aatccctcgc ggcggaagac agcaagctgg tagaaacact caaagccgat
    38881 cacgatgcca cacgccagtt cgtcagcaag ccaatcggta aatctgccga caatatgtat
    38941 agctatctgg cactggtgca ggacgatccg accgtgcagg tggtgaacaa cgcgcaaaaa
    39001 gcgtatgtcg agcattacat tcagggcgat ccggatctgg caaaactgcc agtgctttca
    39061 gctgccgcac cgtttaaagt tggtggtcgc aaaaatgacc cggcaagcta tgtggaggtg
    39121 gaaaaaggcc agttgacctt ccgtaatgcc gccgatcttt atctctaccc caatacgctg
    39181 attgtggtga aagccagcgg taaagaggtg aaagagtggc tggaatgctc tgccggacag
    39241 tttaaccaga ttgatcctaa cagcacgaaa ccacagtcac tcatcaactg ggatggtttc
    39301 cgcacctata actttgatgt tattgatggt gtgaattatc agattgatgt tacccagccc
    39361 gcccgttatg acggcgagtg ccagatgatt aatgccaatg cggaaaggat taagaacctg
    39421 acctttaacg gcaagccgat tgatccgaac gccatgttcc tcgttgccac caataactat
    39481 cgcgcttacg gcggcaaatt tgccggtacg ggcgacagcc atatcgcttt tgcttcaccg
    39541 gatgagaacc gctcggtgct ggcagcgtgg attgctgatg agtcgaaacg tgcgggggaa
    39601 attcacccgg cggcagataa caactggcgt ttagcaccga tagccggcga taagaaactg
    39661 gatatccgtt tcgaaacctc tccgtcagat aaagccgcag cgtttattaa agaaaaaggg
    39721 cagtatccga tgaataaagt cgcgaccgat gatatcgggt ttgcaattta tcaggtggat
    39781 ctgagtaagt aatacacttc tttttcggcc tgcaaatcat caaccgcatc cggcatttat
    39841 tggcggatgc gatgctgacg catcttatcc gccctacaag ccatgaacct tagtccggat
    39901 aagcgcagcg catccggcag ttatgccgca cgttcatccc gcaccgctaa cacctcgggc
    39961 aaattcaact caatccagtc cgccagtgca gcaacctttt cgctcacctg ctcgcccagc
    40021 ggcgtgaggc tatattccac atgcggcggc accaccggat acgcgatacg gttaagaaaa
    40081 ccatcctgtt ctaatgcctg taacgactgc gcaagcatct tttcactcac cccgcccatt
    40141 ttgcgccgca ggtcgctaaa gcgatgagta ccttcgcgta gcgccaccag aatcaacacc
    40201 ccccaacggc tggtgacgtg tttcaacacc tcgcgcgacg ggcactgttc cgcaaagaga
    40261 tttccctctt tcagttgttg cgacaggcta acctgactca tttcatactt acctttttgt
    40321 acgtacttac taaaagtaag tttaagtgtt agcgtattta aacacaagac aaaacgatgg
    40381 agacttccca tgatcgctat tactggtgcc actggccaac ttggtcacta tgttattgaa
    40441 tccttgatga aaacggttcc tgccagccaa atagtggcta tcgttcgtaa tccggcaaaa
    40501 gcacaggcac tgacagcaca aggcattacc gtgcgtcagg ctgattacgg cgatgaagcc
    40561 gcactgacat ctgcacttca gggagtggaa aaactactgc tgatctcttc cagcgaagtg
    40621 ggtcaacgtg ccccgcagca tcgtaatgtt attaatgccg caaaggcggc ggacgtgaaa
    40681 tttatcgctt ataccagcct gctacatgca gatacctccc cgctcggcct cgccgatgag
    40741 cacatcgaga cggagaaaat gttggctgat tctggcatcg tttacaccct gctgcgcaac
    40801 ggctggtaca gcgaaaacta cctcgccagc gccccggcag cactggaaca cggcgtattt
    40861 atcggtgcgg cgggcgatgg caaaatcgcc tcagcaacgc gggcagatta tgcggcagct
    40921 gcggcacgcg tgattagcga agccggtcac gaaggcaagg tttacgaact ggcgggcgat
    40981 agtgcctgga cgttgacaca gttagcggca gagctgacca aacagagcgg caaacaggtt
    41041 acctatcaaa acctgagcga agccgatttc gctgcggcgc tgaaaagcgt tggcctgcct
    41101 gacggactgg cggatatgct ggcggattct gacgttggcg catcgaaagg cggtctgttt
    41161 gatgacagca aaacgctgag caaattgatt ggccgcccaa cgacaacgtt agccgaaagc
    41221 gtaagccatc tttttaatgt taataactag ttaattaaag tggcatcctc ccgcatcctc
    41281 tctgataatg acgggatgcc gggagcaatc atgtctgctt cctgaacttt cttctgacag
    41341 atcaatggat gccagtaatg attagcggcg tgctgtacgc cctgttagca gggttgatgt
    41401 gggggcttat ttttgtcggg ccgttgatcg tgccggaata cccggcgatg ttgcagtcga
    41461 tggggcgtta tctggcgtta gggttaattg cgctacccat tgcctggctg ggacgcgtgc
    41521 gtctgcgtca gttggcgcgt cgcgactggc ttaccgcctt gatgctcact atgatgggca
    41581 acctcatcta ttacttctgc cttgccagtg ccattcaacg tactggcgcg cctgtttcca
    41641 cgatgattat cggcaccctg ccggtggtga ttcccgtttt tgccaatctg ctttatagcc
    41701 agcgcgacgg caaactcgca tggggaaaac tcgccccggc actggtttgt attggcatcg
    41761 gcctggcgtg tgtgaatatt gctgagttaa accacggact ccccgatttt gacttggcgc
    41821 gttatacctc tggcatcgtg ctggcgttag tttccgtggt ctgctgggca tggtatgccc
    41881 tgcgcaacgc ccgctggctg cgggaaaatc ccgacaaaca tccgatgatg tgggcgacgg
    41941 cgcaggcgct ggtcacgctg ccggtttctc tcatcggcta tctcgtcgcc tgttactggc
    42001 cgaatatgca aacgccggac ttctccttac cctttggccc ccgtccgctg gtgtttatta
    42061 gtctgatggt tgcgatagcc gtgctttgct catgggttgg cgcactctgc tggaacgtcg
    42121 ccagccagcg attaccgaca gtgattctcg ggccgctgat tgttttcgaa acgctggcag
    42181 gtttgctgta caccttttta ctccgccagc aaatgccgcc gctaatgacg ctgagcggta
    42241 tcgcgctgtt agtgattggc gtggtcatcg cggtcagagc aaaaccagaa aaacctttaa
    42301 ctgaatctgt ctcagaaagt tgacacgctg gcagtgagtt aaataagcct ctgctacgta
    42361 agggttatag cttttgcctt aaagatgcat ttaaaatgca tcttatatta ttaagaatga
    42421 ggtatcagct atggcttatc gcgaccaacc tttaggtgaa ctggcgctct ctatccctcg
    42481 cgcttcggct ctgtttcgta aatatgatat ggattactgc tgcggcggta agcaaacgct
    42541 ggcgcgtgcg gcggcacgta aagaactgga tgttgacgtc attgaagccg aactggcaaa
    42601 gcttgctgaa caaccgattg agaaagactg gcgtagcgcc ccgctggcgg aaatcatcga
    42661 ccacattatt gtgcgctatc acgatcgcca tcgcgagcag ctgccggagc tgatcctgca
    42721 agcgactaaa gtcgagcgcg ttcacgccga caaaccaagt gtgccaaaag ggctgacaaa
    42781 atacctgact atgctgcatg aagagctttc cagccatatg atgaaagaag agcaaattct
    42841 cttcccgatg atcaaacaag gcatgggcag ccaggcaatg gggccaatca gcgtaatgga
    42901 aagcgagcac gatgaagcgg gcgaactgct ggaagtgatc aaacacacca ccaataacgt
    42961 cacaccgccg ccggaagcct gcaccacctg gaaagcgatg tataacggca ttaatgaact
    43021 gattgatgac ctgatggagc acatcagcct ggaaaataat gtgctgttcc cgcgcgcact
    43081 ggcgggtgag tgatttgttc gatcaacgat cctgacatgg gcaggatcgt tgattattca
    43141 gtcattcagt ggaagtaatt cattgttgta actggtgtac ttaccttcaa cggtaagcaa
    43201 actgccgttt ttatcaagat gcgccgcatc gagttgtcga gtctcattac gccgcttcgc
    43261 atcacgtacc agcccatcgc ctacgtaata aatatagtgg tcatcatgag caaaggtatc
    43321 tgataattga ccattggcgt atttaaacca cgtccattgg cctttcagtt caaaatgctc
    43381 aaccgtgtct tcatcaataa aatacgtata gcgatcgtca tgtgctaaat aatttaatgg
    43441 tgtgccattg gggcttttat accactgcca ttgcccatta atatcaaaaa tagtcacatc
    43501 atcgcgcgcg atgaaataaa ctttgttccc tacaacatta gcacctttct gtaattcttg
    43561 ttgtgggtct ggtaaatgta tgaccgacag cgttttttcg accgcagata tatatttcac
    43621 cagatagagc gaatctttat attgcaccac attctccgtc agcgggcgaa aatattccgg
    43681 atcaacatca ggaattgtcg caacccggca gtcattagca cgcctggaag catcacgcgt
    43741 catccaggtt actttatccc gctgtaggtc aaatatactt cggcaatttt taccgaacgc
    43801 ataacgtttc tccccgtctt tatcgcgata aattagtagt gaaccggcta tccagcgcac
    43861 aatatgaaag gtatccggat cggcgttgat ggcatctcca ttcacaaaga cctgcgattt
    43921 agtccgccag attgtatccc agggtcccgg attacttttg ctttcgcagc tattaggtga
    43981 ataaataatg ggacgacgtg tccagggttt aatgtcaaga atagaatacc cttgcgcact
    44041 acctaaccag cgcccctgaa agtaaagatt gcgacgacct ttcagcacat ccgcggaatc
    44101 aagtgaattc atccttcccc ccacctgttg cagcgaatcc atatcgacct gcttttcgcc
    44161 gtgattatca tccgtgcgtt cgccatcaaa atagaggctg tctttatcgg cagcaaaacg
    44221 cccccatgcg cggaatgttg cggcatcgac tttcggtgtt cccggcgggt tctgaactat
    44281 tttccctgcc cataaaatat ggcggccatc agtcaaccag tcgaaacgcc aaccaccgtt
    44341 ggttctgata taagcgccac catccacccg cacaaaatca ttattgatat catcaggaga
    44401 atcatattca tcatcagtgc cgtcagatac acggcgcagt tgtttgaacc tcacctgcgg
    44461 taaatgaata tccggaaaat agagtccgta accatcgcta tcttcaacaa tatattttgt
    44521 tccttgcccc cacggttggt aataaaaaga ggcagatggt acgccggagc aggctattga
    44581 attgagtgaa aataaagcca gtgcgagaca aacgggttta attggtaaca tagcgtcatc
    44641 ctgatgctgt tagcgtgaat aggttattta aataagggtt taagcccgct cgggggttgc
    44701 ttcaccgata accaacctcc cttgcctaag ttataacgca gccattcacc ttgttgggcg
    44761 tgttctttcc caattccagt cgcttgttga gtgtaaagct gcccgtctgt tgatataaac
    44821 gtgttagtta atccccaact accccctgct tcaagtgagt cggagagatc ctctggcttg
    44881 agattaacca actcaccttc gcccagggcg ttaattgtga ctttatatat ttggttctta
    44941 tataaaccgg tattccgaac aaacagataa aaatattccg gatcaactcc cgccagcgtt
    45001 tcataaatac actcactgcc cgctggagtg gcttcatatt tgagccagga cacgttattc
    45061 agaccaatat taaaaggtgc acagcgatag ctggtatcct ccagctcata gtcacgctcg
    45121 ccgcttttat cgcgataaac taagcgttcg ccaggcatcc aacgtttaat ctgaaaagtt
    45181 ttgcgatcgg cgggcagtgg cttgccatta acaatcacct gcgaatttgt ctgcacaaca
    45241 aattgcaggg aggaatcatg ccgcaggatt tgaaatccgt cagcgcttcc caaccagtgc
    45301 cccttatgcc aaagactatt tttatcacgc agtaaattcc agatgtccgt ctcttcaagc
    45361 gttgacatat ccacctgctt atcaccgcta ttatcatcgg tacgctgacc atcaaaatag
    45421 atactgcgtt tatcagcagc gaaagcgccg aatgcctgaa aactggcagc gtcgactata
    45481 ggtttaccgg ggggattttt caggactttg ccagaccaaa gaacagagcg tccatcggtg
    45541 agccaggcat gttcgcaatc cccctcatca tcgcaatcga acttatcggg attaaaatcg
    45601 gacagtaacc gggaatcttg tttattggct aaattggcaa tacgccaaag gcgggaaaaa
    45661 ttaatatctg gtaattcgga gaggatctca tctttttccc ccttctgttc gacaacataa
    45721 accgtgcctt tctcctgctg gcggtaataa aaatccgcgc cccaggaagg tgaaatagag
    45781 ggaggtaatg cggcgattaa taaccaactg cgcttaacca taatgaactc tgcaacgagt
    45841 ggcaaagatc cacggtaagc tcgcgcctga gggctgtcta tgatggtaga tttttttagg
    45901 aatatataaa gacaaaggca cccgcaggtg cctttcagag ttataaacgc ggcattactt
    45961 cgccatgcgt tttttaccaa taaacagcca gcccagcccc agcgcgataa accacagcgg
    46021 ggtaaccagc agcgcctggc gggtgtcatc ttccagtgtc aacaacacca gaacaaacac
    46081 aaagaacgcc atacataccc agcacatcag cttgccgagt ggcatcttgt agatcgactt
    46141 ctcatgcaga tgaggacgct gtttgcgata caccaggtac gagcaaagga taatcgtcca
    46201 gacaaacata aacagaatcg cggaaacggt tgtaatcatc gtgaacgcgc caatcacgct
    46261 gggattcaca tacaacatca ccacgccgcc gagcagacag atacaagaga aggtcagccc
    46321 tttcgcgggt actgcgcgct tagagagttt agcgaacgct ttcggtgcca caccttcctg
    46381 ggccagacca aacagcatac ggctggtaga gaagacaccg ctgttagcgg aagacgccgc
    46441 agaggtcagc accacaaagt tgatcacgct ggcagccgca ggcaaaccta ccaacacaaa
    46501 cagttcaacg aacgggcttt tctccgggac taccgaactc cacggcgtca cggacataat
    46561 cacaatcagc gagaagacgt agaacataat gatacggatc ggaatggagt taatcgcgcg
    46621 tggcagtgat ttctctggat ctttggtttc cgcagcggtg gtacctacca gctcaatccc
    46681 tacgaaagcg aaaaccgcta tctggaatcc agcaaagaag ccacttaagc ctttcgggaa
    46741 ccagccgccg tcattccaca aatgtgcaaa tgatgcttcc acaccggtcg gtgactgaaa
    46801 gtgcatcgcc accatgacca ggccgacaac aatcagcgac acgatggcga cgattttgat
    46861 catcgcaaac cagaactcca tctcaccgaa catttttacc gtggcgaggt ttagaaccag
    46921 taacagaatg atcacggata acgaagcaac ccagtcagaa agcccaggga accagaattg
    46981 cgcataggcg gtaatggcaa ccacgtctgc cataccggtt acaacccagc agaaccagta
    47041 agtccagccg gtgaaatatc ctgcccacgg cccgagtaaa tcggaagcga agtcactaaa
    47101 agatttgtat tccagattcg aaagcagcaa ttcccccatt gcccgcatca cgaaaaagag
    47161 cataaaaccg atgatcatat aaacgaaaat gatcgacggc ccggcaaggc taatcgtttt
    47221 gccggacccc ataaacaacc ctgtaccaat ggcaccgcca atggcaataa gctgaatatg
    47281 tcggtttgtg agattgcgcc gtagcgactg ttcagccgga gcctgatcat cggcaacgac
    47341 ttttacctga tctaccatgt ttttttcttc ctgtacctgt ctgtgttgtt caggctctac
    47401 ggcctttagt cagtctatga tacgacgaat ctgtatcggg actcatcgat attaggtaac
    47461 aatacgcggg atgaatacta agatttagat ctaatgttaa ttttatgttt aaagtgagtg
    47521 tcatatcacg gtgataacgc gaaacagctc acaaaaatac acgcttacgg ggtctttgca
    47581 acataaaata gcgccgtttg gcaaactgaa gggtttattg ctgaatgcct gctcccctct
    47641 ggttcgaggg gaacagaatg cgctgcttag aggatttcca gcagttcgac ttcaaacacc
    47701 agggtgctga acggagggat ggacgcgcct gcgccacgct cgccatatgc cagttcctgc
    47761 gggatagtca gttcccattt agaacctacc ggcatcagag tcagtgcttc aatccagcca
    47821 gggatcacgc cgttaaccgg gaattcagct ggctcgccgc gagcaacgga gctgtcaaac
    47881 acggtgccgt cgatcagttt accggtgtaa tgaacacgaa cgcggtcggt acgtgccgga
    47941 attgcgcctt caccctggtt gatcacgcgg aattgcaggc cagattcggt gctatttaca
    48001 ccttcttttt tggcgttttc ttccaggtat ttcacacctt cagcagccat cgcctggaaa
    48061 cgctgacgac gaacggcatc ggcgcgttcg tggatttcac gcagcgcgcg gtgcaccaca
    48121 tcaaccggaa cagccggatg tttgccttcc agcgcatcag caatgcctgc aaccagtgct
    48181 tctggcagca gcccttccag gccagattca ctcagttgtt gccctacctg caaaccaatg
    48241 ccgtagcttg cttgcgcttc gatggtgtca aaagttgggg tggtcatcgt atttcctttc
    48301 ttagggtata aagtagcggg cagcatatca gccacacttc tttgggtaaa actttgtcgc
    48361 gggaatgatg acaaatcccg gggaaaaaga aacaatagca atatcccgta tgaatataat
    48421 aaaggcgggt ttaccacgga catcatagcg ttagcttatt cataggctat gattgaggaa
    48481 caagacgcgg agcaggagga aaccatgccc gggcgctttg aactaaaacc aaccctggag
    48541 aaagtctggc acgcgccgga caattttcgc ttcatggacc cgctgccgcc gatgcatcgt
    48601 cgggggatca ttattgccgc cattgtattg gtggtcggat ttctgcttcc atctgatgat
    48661 acgcccaacg cgccggtggt gacgcgtgaa gcgcagttag atattcagtc gcaatcacaa
    48721 ccgccgacag aagaacagct gcgggcgcag ctagttatgc cacaaaatga tcctgatcag
    48781 gtggccccgg tcgctccaga accaatacag gaaggccagc cagaagagca gccgcaaatc
    48841 acccaaacgc aaccattcca gccagatagc ggaattgata atcaatggcg ctcctatcgt
    48901 gtcgaacccg gtaaaacgat ggcgcagctg ttccgcgacc acggactgcc cgctaccgat
    48961 gtctatgcca tggcgcaggt agaaggcgcg gataagccac tgagtaattt gcaaaacggg
    49021 cagatggtga aaatccgcca gaacgccagc ggcgtggtga cgggcttaac cattgatacg
    49081 ggaaataatc agcaagtcct gttcacccgc caacctgatg gtaatttcat ccgcgcacgc
    49141 tgattaccca cgggcagggt tcaccaaaag ccagctcagg atggtctctt tgatcaccct
    49201 gcgtctggct tccaaagcct caggtgaaac catgctaatg tgataaacca tccccagtgt
    49261 gtactggttt attaaataat atgcacccag tgcggcgata ttaatgttga tttgtaaggg
    49321 atcaatatct ttcttaaagg ttttctgccg ctgcccttct tctaataatg aagccattga
    49381 ctgcaaatgt gcatggttaa tttcagcgag gcgttttgat tttttataat gcacaccctt
    49441 actttggttt tcactatgga caatttttaa aaaccacggg ttattgatgt aataatccca
    49501 agtaaaatca attattttct cgatcgcaat aaccggcgaa agccctgaaa tgtcaagctc
    49561 tctctctttt tcgcgaatat cgttccaggt atattccagt acttcaataa acaaattttc
    49621 tttattacca aagtggtgat aaaccgtctg tttactgcac cctgctgcct taacaatatt
    49681 atctactctg gccccttcgt aaccatattc ggcaaattca ttaaccgcgc atagcagcaa
    49741 tttttccttc aggttttgtg cgtgactggt atcaagatac attccacttc cccacttcct
    49801 cttcgtgatg gctatcataa ccggatttga tctcagcgac agcaaaaagg ataaaagtcg
    49861 taatttcatt gagttatcaa attacctgtg atcactctcg catatcaacc aactagatgg
    49921 ttgatctttt tgcattccag tcgcaagcta atcatcactc aaggaaacaa tattcaaaag
    49981 agggtcattg tatgaaatcc actcgtcctg ttgctgtcat cactggcgct gcacgtggta
    50041 ttggaaaggg ttgtgcgctg gaacttgctc gtagcgggtt taacctgctg attaacgatc
    50101 gccctgatgc cgacagcgta gaaaaactgc atatcacaca acaagaatgt atggcagaag
    50161 gcgtggaagt gatttgcttc cctgctgatg tgggcgatct ctccctgcat gaagagatgc
    50221 tcgacgcggc gcaaaaccag tgggggcgtc tggactgcct gctcaacaac gcaggcattt
    50281 cagtgaaaaa acgcggtgac ctcctcgacc tcgaaccaga cagcttcgac caaaacattg
    50341 ccattaacac ccgtgcgccg ttcttccttg ctcaggcatt cagtaagcga ttactggcac
    50401 agccgaaacc ggaagcagaa ctgccgcacc gctcgattat ttttgtcagt tccataaacg
    50461 ccatcatgct ggcgatgaat cgcggcgaat ataccattgc caaaactgcc gtctctgccg
    50521 ctgctcgtct gtttgctgct cgcctttgta atgaacaaat tggcgtttat gaagtgcgcc
    50581 ctggtctcat caaaaccgat atgactattc cggcaacggc ttattacgac gaactgattg
    50641 ccaaaggtct ggtgccgtgg ggacgttggg gttatccggc ggatatcgct tccactgtcc
    50701 gcgcgatggc ggaaggtaaa ctgatttaca cctgtggtca agcggttgcc attgacggtg
    50761 gcttaagtat gccgcgcttc tgaggctgaa tcatgagaga tctgttacaa cgcccggatt
    50821 tattcagtat caataccgcg acactgggct ataaaacgcc gctacccgcc attattgatg
    50881 cctgcgccgc acgcggtatt ggcgcgattg ccccctggcg cagggagttg cagagtgaaa
    50941 atctccagca aatcgcccgc cagcttgctg ccagtaacat gaacgtttcc ggtttgtgcc
    51001 gtagcaccta ctacaccgca ccgacgctgg cagaacgcaa actggcgatt gatgataacc
    51061 gccgggcgct ggacgacgcc gctgtactga acgccgcctg ttatatgcag gtggttggcg
    51121 gtctgccaat gggaacaaaa gatctgtatg aagcgcggga acaggtgaaa caagggattc
    51181 gccagttgct gccacattcg aaagatgtcg gtgtgccaat tgcgctggag ccactgcacc
    51241 cgatgactgc tgccgatcgt tcttgtctgt gcacgttacg tcaggcgctg gactggtgtg
    51301 atgaactcga tcccgatggc gaatttggcc tcggcgtggc ggtagatgtc taccatgtct
    51361 ggtgggatcc ggatctcgcc agtcagatcc tgcgtgccgg aaaacgcctc ctcgcgtttc
    51421 atgtttccga ctggttaata ccaaccaccg atctggtcaa tgaccgggga atgccgggag
    51481 atggcgtcat taatattccg tcaattcgtc ggctggttga aaacgccgga tttaatggcg
    51541 ctattgaact ggaaatattc tcaccgtact ggtggcagaa agatattaac agcacgctgg
    51601 atattagcgt cgatcgcatc gcgcattatt gctaaggaat aatcatgagt gaccaacccg
    51661 ttctgttcag cagagcggcg gcttcttgcc gcctgacatt gaaccgtgaa gataaatgtc
    51721 atgctattaa tgaagaaatg atcgaatctc tcgaccatta tttaaatgaa attgaaaatg
    51781 acacggcatt acgcttagtc gaattaacgg caaccggcga taaattcttt tgtgcgggcg
    51841 gcgatataaa atcgtggtcg gcctattcgc cattagatat gggcagaaaa tggattaaac
    51901 gcggtaacga ggtttttaat cgcctgcgca atttacccca attaacggtt gcaaatctca
    51961 acggtcatac cattggcggt gggattgaac tcgccttgtg ctgtgatatt cgtatcgctc
    52021 gcccccacgc aaaattctct aatcctgaag tgatgctcgg catggtgcct ggctggatgg
    52081 gcattgaacg cgtgcttaat caggttggcc cggtggtcgg tcgccagatg ctgatgctgg
    52141 gtaaacgcct gacggcgcag gaagcccagg tggcgaattt aattgatgaa gtggtagaaa
    52201 aagaacaggt ggaaagctgg atggcgaacc agttagcgca gctggaaaaa tgtggtccgg
    52261 tggcgcttgc acatattaag caactgattc tggcactgga aaataaacat gccgattacc
    52321 cgcaccagct actggccgga ttaatgtccg ccactcagga ttgccagcag gcgacgcgcg
    52381 cctttgccga aaaaagtagc gtgagcttcc ataatcaata aggaaattgt atgcgtaaga
    52441 taacaaccgc cgaggcactg gcggcgcaaa ttcaggacgg tgcgactatt gctattagcg
    52501 gtaacggcgg cggtatggtg gaagccgacc atattctggc tgctattgaa gcgcgtttcc
    52561 tgcaaaccgg acacccgcgc gatctgacat taatccactc gctgggtatt ggcgatcgcg
    52621 actgcaaagg cactaatcgt tttgcgcacg cagagatgct caaacgcatt attgccgggc
    52681 actttacctg gtcgcccaaa atgcaggctc tggtaaaaaa taacacgatt gaagcctact
    52741 gctttcctgg tggcgtgatt caggcactgc tacgggagat cggcgccgga cgtccggggc
    52801 tttttaccca cgttgggctg ggatcgtttg ttgatccacg caatggcggc ggtaagtcga
    52861 acgaatgcac taccgaggat ttggtagaac tgattgaaat cgacggtgaa accaaactcc
    52921 gctatcgccc tttcaaagtg gactacgcga ttttgcgcgg cacctatgcc gatccgcgtg
    52981 gcaacgttag cctcgaagaa gaagcgattg atatggatag ctattcgatg gcgctggcag
    53041 cacacaacag tggcggcaaa gtgtttgtac aggtgcgcga tgtactggaa gctggcgcca
    53101 ttgaacctcg caaggtcaag ctaccgggga ttctggtcga tggtatcgtt gagcaccgcg
    53161 aacaaccgca aacctatctt ggtggttacg acctgaccat tagcggtcaa catcgccgtc
    53221 taagttctaa cgacgctatt gaactggtta gtcatccggt gcgtcgcctg attgcccgtc
    53281 gggcagcgcg ggaactggcg gcaggcgctt caaccaactt tggctttggt attcccggtg
    53341 gtattcctgg ggttgcgctg cgcgaaggcg tgccttatca aagtttgtgg ctgagtgtag
    53401 aacagggtgt acataacggc atgatgctgg atgatgctct gttcggctgc gcccgtaacg
    53461 ccgatgccat tattccatca ctcgatcaat ttgaattcta cagtggcggc ggcatcgata
    53521 tcaccttcct cggcatggga gagatggatc aatacggtaa cgtcaacgtc tcccatctta
    53581 atggcaatct cattggcccc ggcggcttcc tcgaaattgc acaaaacgcc cgtaaagtgg
    53641 tgttttgcgg tacgtttgac gccaaaggca gcaagattga tgtaacgcca gatggcttgc
    53701 atatcgccca gtcaggtcaa atccctaaac tggttaccca ggtggaaaaa atcactttta
    53761 gcgccgccta cgcacagcaa agtggtcagg aagtgttgta tatcactgaa cgtgcagtat
    53821 tccagttaac ggcagaaggc gttgaattaa ttgaaatcgc gccgggtgta gagattgagc
    53881 gcgacattct gccgtatatg gctttccgtc cgattatcag gcacccacgc ctgatggaaa
    53941 gtagcctgtt tacgccgatg gaggatgcat gagtcagctt gatgacacca tccttgatgc
    54001 gctgacgtac gtcactttcc caaaggggtt tgcgtcagca gagccaacgt caattgtcac
    54061 cgttgaggat gttgattacc cgatctggca agttgatgcg ctggtcgttg gtagcggcgc
    54121 agcaggatta cgtgcggcag ttgaacttaa acgccgccag caaaatgtgc tgatcgctac
    54181 cgccgggtta tatatgggga cgtcggcatg ttctggttcc gataaacaga cactatttac
    54241 cgccgctacc gcgggcaacg gcgacaactt caccaaactg gcagaggcac tggcgagcgg
    54301 tggggcgatg gatcacgaca ccgcttatgt cgaagcggta ggttctctgc acactctagg
    54361 cgggttgcaa tatcttggtc tggaattacc ggaagatcgc tatggcgcga ttcttcgtta
    54421 tcaaaccgac catgacgaag ccgggcgtgc aacctcgtgt gggccgcgta cctcaaggtt
    54481 aatggtgaaa gtgctgcagg aagaagtaca gcgtctcgcc attccagtgt tgaccagtgc
    54541 aacagtgatt aaactgctgc atcagcgtga cgaaaacggt gaagaccgtg tggtgggggc
    54601 aatcctcgcg accggtcatc gcgcccataa cccatggggg ctggcaattg tgactgcgcc
    54661 caatgtggta ctggcaacag gagggcctgg cgagctttat cgcgacagtg tgtacccaca
    54721 taaatgcttc ggctcgctgg ggctggcgct ggaggaaggt ctgacgctaa ccaatctgac
    54781 cgaaagccag tttggtatag gcacaccgcg cagcaccttt ccgtggaatt tgtccggcac
    54841 ctatgtgcag gtgatcccgt atatctattc cgtggatgct gagggtaacg agtataactt
    54901 cctcgcggat tactatcgca ccacccagga gctggcttca aacatcttcc gtaaaggcta
    54961 tcagtggccg ttccacgcca cgcgggtaat ggattttggc tccagcttgt tagatatggc
    55021 ggtggcgcaa gagcagcaat cagggcgtca ggtatttatg gatttcaatc gtaatccaga
    55081 acctgtgccg ggtgatttgc ccttctcatt agatcgactg gacgacgacg ttcgcgcgta
    55141 tctggaaaat aacgacgctc tggcaccatc gcccatcgaa cgactgcaac gaatgaatcc
    55201 gctgtctatc tcgctgtata agatgcacgg ttacgatctc accacgcagc cattgcagtt
    55261 tgccatgaat aatcagcata tgaatggcgg cattgaagtg gatatctggg gacaaacatc
    55321 tctgcccggt tgttttgcag tgggggaagt tgctggcaca cacggcgtta cccgacctgg
    55381 cggtgcggcg ctgaatgccg gacaggtatt tgccgtgcgt ctggcacgtt ttattggttg
    55441 cacgcaaaag cggaatattg atggagatat agcacagctg gcagctccgg cactggcttc
    55501 tataagagag atcattactc aggcgcacga taacgggacc gggatgccgt tgtcggttgt
    55561 gagagaaaaa attcaggcac gaatgtctga ccatgcggga tttatttgtc atgccgatag
    55621 agtccgacgc gccactcgtg atgccctgct attgaacgaa tttgtccaac ggcatggatt
    55681 ggcaatcaaa catgtgggtg aagtttccga gctgtttatg tggcggcata tggcgctgac
    55741 ttctgccgcg gtattaacac aattaacgca ttatattgat gctggtggcg gcagtcgtgg
    55801 ggcgcggatg gttattgatc cacaaggcaa atgcctacca caaactcgtc gcggcgtaaa
    55861 agaagaatgg cgttttcgct ctgaactcgc tgaagataaa aatcacaaat taacaattca
    55921 atattcgcaa ggttctttta ttaccgaagt aaagtcttta cgtatgcaac cgtgtattaa
    55981 cggtatttac tttgaaaaaa actggccaga ctttttaaag ggagacattt acacacaata
    56041 attttcaata tttcctctgt acctgatttt tattg
//
LOCUS       DAOFQD010000013        56035 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.013, whole genome shotgun sequence.
ACCESSION   DAOFQD010000013 DAOFQD010000000
VERSION     DAOFQD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.013"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(216..2177)
                     /gene="topB"
                     /locus_tag="QBF46_000832"
     CDS             complement(216..2177)
                     /gene="topB"
                     /locus_tag="QBF46_000832"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDQ6673059.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRLLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLMENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(2182..3225)
                     /gene="selD"
                     /locus_tag="QBF46_000833"
     CDS             complement(2182..3225)
                     /gene="selD"
                     /locus_tag="QBF46_000833"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDQ6673060.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3342..3893)
                     /locus_tag="QBF46_000834"
     CDS             complement(3342..3893)
                     /locus_tag="QBF46_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDQ6673061.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4054..5910
                     /gene="sppA"
                     /locus_tag="QBF46_000835"
     CDS             4054..5910
                     /gene="sppA"
                     /locus_tag="QBF46_000835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDQ6673062.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGDDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6077..7093
                     /gene="ansA"
                     /locus_tag="QBF46_000836"
     CDS             6077..7093
                     /gene="ansA"
                     /locus_tag="QBF46_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDQ6673063.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELHEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7104..7745
                     /gene="pncA"
                     /locus_tag="QBF46_000837"
     CDS             7104..7745
                     /gene="pncA"
                     /locus_tag="QBF46_000837"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDQ6673064.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7838..9196)
                     /locus_tag="QBF46_000838"
     CDS             complement(7838..9196)
                     /locus_tag="QBF46_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6673065.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLH
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRMRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(9313..10071)
                     /locus_tag="QBF46_000839"
     CDS             complement(9313..10071)
                     /locus_tag="QBF46_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6673066.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(10208..11188)
                     /gene="ydjG"
                     /locus_tag="QBF46_000840"
                     /pseudo
     CDS             complement(10208..11188)
                     /gene="ydjG"
                     /locus_tag="QBF46_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
     gene            complement(11198..12145)
                     /locus_tag="QBF46_000841"
                     /pseudo
     CDS             complement(11198..12145)
                     /locus_tag="QBF46_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            complement(12150..12985)
                     /locus_tag="QBF46_000842"
                     /pseudo
     CDS             complement(12150..12985)
                     /locus_tag="QBF46_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
     gene            complement(13006..14049)
                     /locus_tag="QBF46_000843"
     CDS             complement(13006..14049)
                     /locus_tag="QBF46_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDQ6673067.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATHPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTIKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQMVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(14066..15445)
                     /locus_tag="QBF46_000844"
     CDS             complement(14066..15445)
                     /locus_tag="QBF46_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6673068.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASTVYVPEIWPTEAKLRGSG
                     LANAVGRISSIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(15472..16548)
                     /locus_tag="QBF46_000845"
     CDS             complement(15472..16548)
                     /locus_tag="QBF46_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDQ6673069.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPTCEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(16918..17190)
                     /locus_tag="QBF46_000846"
     CDS             complement(16918..17190)
                     /locus_tag="QBF46_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDQ6673070.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(17232..17645)
                     /gene="msrB"
                     /locus_tag="QBF46_000847"
     CDS             complement(17232..17645)
                     /gene="msrB"
                     /locus_tag="QBF46_000847"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDQ6673071.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            17987..18982
                     /gene="gapA"
                     /locus_tag="QBF46_000848"
     CDS             17987..18982
                     /gene="gapA"
                     /locus_tag="QBF46_000848"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDQ6673072.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            19066..19950
                     /locus_tag="QBF46_000849"
     CDS             19066..19950
                     /locus_tag="QBF46_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDQ6673073.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(19998..20852)
                     /gene="yeaE"
                     /locus_tag="QBF46_000850"
     CDS             complement(19998..20852)
                     /gene="yeaE"
                     /locus_tag="QBF46_000850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDQ6673074.1"
                     /translation="MQQKMIQFSGDVSLPAVGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(20942..21688)
                     /gene="mipA"
                     /locus_tag="QBF46_000851"
     CDS             complement(20942..21688)
                     /gene="mipA"
                     /locus_tag="QBF46_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDQ6673075.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDSTDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            22124..24058
                     /gene="yeaG"
                     /locus_tag="QBF46_000852"
     CDS             22124..24058
                     /gene="yeaG"
                     /locus_tag="QBF46_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDQ6673076.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            24171..25454
                     /locus_tag="QBF46_000853"
     CDS             24171..25454
                     /locus_tag="QBF46_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDQ6673077.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            25601..27076
                     /locus_tag="QBF46_000854"
     CDS             25601..27076
                     /locus_tag="QBF46_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDQ6673078.1"
                     /translation="MIQSTRISLGLSFNYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            27257..28747
                     /locus_tag="QBF46_000855"
     CDS             27257..28747
                     /locus_tag="QBF46_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDQ6673079.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            28790..29293
                     /gene="yeaK"
                     /locus_tag="QBF46_000856"
     CDS             28790..29293
                     /gene="yeaK"
                     /locus_tag="QBF46_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDQ6673080.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(29294..29398)
                     /locus_tag="QBF46_000857"
     CDS             complement(29294..29398)
                     /locus_tag="QBF46_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673081.1"
                     /translation="MNDQMFVEILIITSSFFAIAVVLVLSVLLIERTG"
     gene            29568..30014
                     /locus_tag="QBF46_000858"
     CDS             29568..30014
                     /locus_tag="QBF46_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDQ6673082.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(29971..30792)
                     /gene="nimR"
                     /locus_tag="QBF46_000859"
     CDS             complement(29971..30792)
                     /gene="nimR"
                     /locus_tag="QBF46_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDQ6673083.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            30889..31440
                     /gene="nimT"
                     /locus_tag="QBF46_000860"
                     /pseudo
     CDS             30889..31440
                     /gene="nimT"
                     /locus_tag="QBF46_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            31613..32821
                     /locus_tag="QBF46_000861"
     CDS             31613..32821
                     /locus_tag="QBF46_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6673084.1"
                     /translation="MAKIKLTKSAVDAAQAQAQPVELRDTLVPGFLCKITPAGRKVFM
                     LQYRTNAGERRKPALGLYGELTVEQARSLAQEWLAQVRRGGDPAAEKAAARQAPTVKE
                     LCIKFMEDYSSQRNKPSTQEGYQSVIDRNIIPMLGRMKVQDLKRPDVASMMKKMAHKP
                     ADANRTFSVMRKMFNLAEVWGYRPDGTNPCRHVPMYPNGKATHLISDEEMGKLFRYLD
                     QLEAEGLEHVVIPLAIRLQFEFAARLSEIVSLQWDWIDLENRRVVWPDSKTGGMSKPM
                     SEEAYRLLSTAARNATTPHVLPSPRHPDRHLTDGEYYNGWTRTLKAAGVTHVGTHGIR
                     HRSATDIANSGIPVKVGMVLTAHKTVAMFMRYVHTEDKPVREAAELVASRRKRITGAK
                     QRPPAAEATA"
     gene            32818..33885
                     /locus_tag="QBF46_000862"
     CDS             32818..33885
                     /locus_tag="QBF46_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDQ6673085.1"
                     /translation="MTRRKASIASPAAPMALLGDIRALIEASRQRAASTVNAELTLLF
                     WQIGQRIHMEVLAGQRAGYGEEILPTLAEQLARDYGRGFGEKNLRRMVKFAATFPEEP
                     IVATLSRQLSWSHFVLLLALKEPLQRDYYAQMAGTERWSVRTMRERIDSMLYERTALS
                     QKPEETIAQELATLRDAQRMTPALVMRDPYILDFLGLRDSWQESDLEAAIIREMEAFL
                     LELGAGFCFVARQKRIQIDDEDFHLDLLFYNRKLRRLVAVELKIGEFKAAHKGQMELY
                     LRWLDKHEREPEEASPLGIILCTGKKREQIELLELDKSGIHVAEYLTSLPPRAVLGEK
                     LQQATERARLQIEQRQPGEKS"
     gene            33999..34268
                     /locus_tag="QBF46_000863"
                     /pseudo
     CDS             33999..34268
                     /locus_tag="QBF46_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            34364..35668
                     /locus_tag="QBF46_000864"
     CDS             34364..35668
                     /locus_tag="QBF46_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010793081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISPa33 family transposase"
                     /protein_id="HDQ6673086.1"
                     /translation="MLPEKLHFSPLSRRLVEASFTGGHITSDAGLLLLREVDRQHGLT
                     RRLAKIVPDRRDPGRVQHGLQTLLAQRIYALAAGYEDLNDHDGLRHDYALQTAVNRLQ
                     PLAGKSTLGRLEQQADRETVVQAHRLLWEHFIAQHDQAPAEIVLDFDATDVPVHGDQE
                     GRFFHGYYDHYCFLPLYVFCGRHLLVSYLRPSNIDGARHSWAILALLVKFIRRFWPET
                     RIVFRGDGGFCRHRMLDWCDRKQVDYVVGLARNTRLQRMAAPAMQAVAEAYQETGQKM
                     SGVYRFLYQAGSWRRRRLVVSRLEHGEQGANPRFIVVSRFAEDAAQQYYEDYCGRGDM
                     ENRIKDQQLDLFADRTSSPRWWTNQWRLMLSAFAYVLFERLRDHLQGTALARMSIHNL
                     RLKLLKIGAVIIRNSRRIRVLISEAYPHQELFAGLVARLKPT"
     gene            complement(35903..36457)
                     /locus_tag="QBF46_000865"
     CDS             complement(35903..36457)
                     /locus_tag="QBF46_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDQ6673087.1"
                     /translation="MSKKPEVFISVDVETAGPIVGEHSMLTVGACLVYQPEISFSIML
                     KPVSEKSVAEALKITGLTLEQAENEGVTPSEAMTEFSAWIAKNVPKESSPVFVGLNAP
                     FDWGFVNYYFLKYLGENPFGFTALDIKALFMGATGCSWYETRSSAIDKVVHPNQKGDH
                     DALHDAKYQAELFRLVRALAERGD"
     gene            complement(36485..37381)
                     /locus_tag="QBF46_000866"
     CDS             complement(36485..37381)
                     /locus_tag="QBF46_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ6673088.1"
                     /translation="MSNSSVFEVKTNLPTTQLDIQAQKLLGFEGRYQRVQKRLQLILQ
                     SNELERWSQKHHKKKLAVIELLNDQYPLAIFHGDVGTGKTANAEAIANRLARDARIED
                     AILFKLSNKVRGTGKVGEMGTLITQAFEDISRSIGPGKGRAFLIIDEGDSLGASRSQD
                     HSHHEDKVGVNTLIQSIDSMRKHGGRIFAILCTNRLAALDAAVIRRASIVEEFTRPTD
                     EERNELFTNDLGDMGFSATEIKKLVEATSATKTKPAWTYSDIRTRLYPAAVSLAFPNS
                     PLKAEHFHQAVAELEPSPVMAG"
     gene            complement(37378..37866)
                     /locus_tag="QBF46_000867"
     CDS             complement(37378..37866)
                     /locus_tag="QBF46_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673089.1"
                     /translation="MSTYSSTNTYTVADVEKVMRSVKADLIIIATTTKAMTEDEASNY
                     AHDIELLAKNGYLEYADVTLMNGTSEVRAIKYEFQSEGATGTERPGGVTWPLTPKDNG
                     GWIRIHLRYTNSSSAEKRADLPLKISWVPSSTDTSHKDLTSSAGRGYSSNGFGTNRKD
                     FS"
     gene            complement(37872..38819)
                     /locus_tag="QBF46_000868"
     CDS             complement(37872..38819)
                     /locus_tag="QBF46_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDQ6673090.1"
                     /translation="MPLSNSELEYFDHNVLRLPAEKRKEYHAQVDNLVVELKKRITDK
                     SKIKVTKVVKAGSFAKYTILRKVDDYPTDVDVVFYVTGVDEDGKSYENLCNKIYDLLI
                     EIYPTKKVEDFEIQRRAAKVTFVKSGLEVDVVPVLQDSSNADHGWQYDIQSGAKNLTC
                     APCHIQFVKTRKDKDKHFRTLVRLAKRWRHFHDVPGLKSFHIELIFGYLLDRDGPAVT
                     IEKRFREFLAYIASTKLGERIDFPENAGKPTSNFDTPVIIIDPVSPENNVASRITKDE
                     QALIAKAAESSLDTATYASVENDNEVWKEIFGGRFKTKD"
     gene            complement(38856..40391)
                     /locus_tag="QBF46_000869"
     CDS             complement(38856..40391)
                     /locus_tag="QBF46_000869"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013232.1,HMM:NF036729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine phosphorylase"
                     /protein_id="HDQ6673091.1"
                     /translation="MTNSLMLASSHILLAGSISNTTENELIDRAHAFVDTLVEEVLNA
                     GGGFVIYVAAEPVNGDGKALLFDWTVAKAVDRLTPGNSSHIRLKIVASHERLQVKANP
                     EQRKLLMSMVARGVAEVVPLEEDVLTGGNVGDEQIEHATAMVALGGGKGVLDRARKMT
                     KKLLPVLPLDLQLGANSEDGLGALGVLKNFQTNPLTYMPNSGNKVVKFLPAISLQEPV
                     LALSEISARIVKIFYEEERARIDALPPDVLVLTALDVELAAAKQAFGIAEDAEHTTTE
                     NGLHVWKAPVVKRGGKTASCALACFAGAGNVDAASVTSMLLSDLRPGNVMMLGIAAGL
                     REKCALGEVVLAERIVAYEGAALVEGGKSEARPEITRLTMRVRQDVSSYLSNRTGLEA
                     RLLKSYQALGIELPEQVDAGPVAKGVLPKTATVASGEKLLRDPEKFLGMREIHGKIEV
                     AEMEGAGLFAACANFGKPVLMVRGISDFGDSKKDNRFHLLAAQAAAAVTVDYIANGMT
                     LQD"
     gene            complement(40634..41038)
                     /locus_tag="QBF46_000870"
     CDS             complement(40634..41038)
                     /locus_tag="QBF46_000870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673092.1"
                     /translation="MTNQKFNPNLGLIKSCIGIQMTTKYPSIETVALRLAVLLKRTGK
                     TRARVSDKTLRILSGRTTLRDAFVINMRSVLEEYGVVAVRLDRGGFALIATSALEGAP
                     PALVKNLMPDFKRLTEEELWNELELPDDEVNE"
     gene            41537..42364
                     /locus_tag="QBF46_000871"
     CDS             41537..42364
                     /locus_tag="QBF46_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDQ6673093.1"
                     /translation="MLASRFASRSPVLRSDSPLSDDQIHRVAPSIFAEAPHESRSQRY
                     AYIPTATVLTELRKEGFQPFMVTQTRTRHEDRRDYTKHMIRLRHASQINARGEANEII
                     LLNSHDGTSSYQMLAGMFRFVCSNGLVCGDTVADVRVPHKGDVAGQVIEGAYQVLHGF
                     DRALESRESMQAITLNEGEAEVFARAALSLKYDDPDKPAPITESQILMPRRFDDRRPD
                     LWSVFNRTQENLTKGGLHGRSANGRRQQTRPVQGIDSDIRLNRALWLLADGLRQLKA"
     gene            42446..44530
                     /locus_tag="QBF46_000872"
     CDS             42446..44530
                     /locus_tag="QBF46_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDQ6673094.1"
                     /translation="MNAINYTEAQAIHATASTAPAVLLEAADLSKNLILVPLSRLVSR
                     PTGRNVRKTPRMSIPELAASIQRVGLLQNLIVTSTADGERYEVVAGGRRLAALKLLAK
                     KHHISKEWEVPCLQVADGTARTASLTENVQREAMHPADQFLALAALVAEGRPIEDIAA
                     DFSVTPLVVQRRLKLANVSPRLLADYRAEAVSLDQLMALAITDDHAAQEAAFYDAPTW
                     QRSPHNLRDRLTEREIDAHRHPLVRFVGLDAYEAAGGGARRDLFAEADSGVYLTDATL
                     LERLAQDKLASLAAEVKAEGWAWVDAAPGTTHADLQAFQRAPRQRRIPNKREAQRIEK
                     LQTKMQALAEAVDAALDADDEEKADALQEEGEHLGEQLQALEDGLQDYGEAVKAAAGA
                     IVTIDRNGEAVIHRGLLREAEAKALRTLEQLRQGFGREGEAGNDDTGEETDDAPKPAA
                     MSDRLAQRLSAHRTAALQIEVARHPQVALAALVHGMVQTVLQGSHYGHDLPLGVKLTQ
                     QDRLEGMAPDWPESPAAVALRELQQLAGEALPEDSTELFAALLAKSQDELVRLLAVCV
                     ASTVDVVTPRATPHQPGTELAQAVGLDMAAWWKPTADGYFQHVPKAAILEAVGAFASS
                     HVTRLAKLKKGDIASEAERLADGTGWMPAIFAAEATQQAAHEVVTDEAAEAPEEVAAV
                     ADEQPQAEALAA"
     gene            44592..44801
                     /locus_tag="QBF46_000873"
     CDS             44592..44801
                     /locus_tag="QBF46_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673095.1"
                     /translation="MSHNTTNRPRIAATYAPGTIRARRWHGDGDVRGYRPPHGWIARA
                     DLTDLHPITGRALPRAVWWIIETKE"
     gene            45577..45891
                     /locus_tag="QBF46_000874"
     CDS             45577..45891
                     /locus_tag="QBF46_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HDQ6673096.1"
                     /translation="MANIGTFTAEKDGFTGTLRTLTFNVKVKLVLNDKGDNESASDYR
                     LQAAGHDIDAAWKKTSEAGRPYVSVTLDDPSFPATVYARLIEGEDGAHDLIWSRSKPK
                     AA"
     gene            complement(45934..46830)
                     /locus_tag="QBF46_000875"
     CDS             complement(45934..46830)
                     /locus_tag="QBF46_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HDQ6673097.1"
                     /translation="MAKDLFSEINNAVLDLQSSQLQTYERPLIKLAQLLRHPDLEPYN
                     AELTEGLDVEAFIAESEKTGGSMVGSAQLAWPDDPKQALGLTLLLIEKLAADPGYATN
                     FGHHFFYSGKKVIAGIHALTGQLIIPFVRDYKSYIQAKGSTDAMLKAQFSRKVFIVHG
                     HDDGARETVARFLERIGLEAIILHEQANQGRTIIEKVVAHSDVGFAVVLLTPDDEGCV
                     KGGTPEPRARQNVLLELGYFIGRLGRDKVCALKRGAVEIPSDFAGVVWETMDSGGGWK
                     QALARELEAAGHSIDWNKVMRA"
     gene            complement(46830..47117)
                     /locus_tag="QBF46_000876"
     CDS             complement(46830..47117)
                     /locus_tag="QBF46_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014859395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673098.1"
                     /translation="MFKITGLDKLQKNLKDAQRALSELDGELGVVNFDPNDPASIEGA
                     IQSVNRMIDDRLGEHSSNPIVGPLAEQMKEKYRESILEKAGEARLESDGDD"
     gene            complement(47131..47424)
                     /locus_tag="QBF46_000877"
     CDS             complement(47131..47424)
                     /locus_tag="QBF46_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019439200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6673099.1"
                     /translation="MTAFKSSLPDALRRIRKARGLSQEAFSDVSSRTYLSSLERGLKS
                     PTLNKIEDVCAVLDVHPLTLLVLAYAGSDPKNVHELMDHIAREVSSFSEHPNK"
     gene            47696..48043
                     /locus_tag="QBF46_000878"
     CDS             47696..48043
                     /locus_tag="QBF46_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2958 domain-containing protein"
                     /protein_id="HDQ6673100.1"
                     /translation="MTPLITADERTRLLANGQARTAGQDTDPLPVVRLFTPDAHATWL
                     LASLDPADGDTAHGLIDLGIGMPSLGTVKLSDLAAIVGPRQQPVMRDRYFQPVRRLSE
                     YLRLAEDSGSITD"
     gene            48365..49171
                     /locus_tag="QBF46_000879"
     CDS             48365..49171
                     /locus_tag="QBF46_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HDQ6673101.1"
                     /translation="MADRSAEPWYATAAYLYVLHLDGPALAWEYLRRHPDYRRDWLRR
                     RRQPDAAERWGLRLLEDPALDARDAQPAWFPDHDAVVQLYPDADPPPDALFFEFWKLP
                     GHKHLIHDGKRLVLVARWPGCCLRFVLAPGLADGMAYAYAIRACAAPCERYAALASLL
                     NKIAVAAGAAPAATVRPRPPLSALLELHTLQALDATLAGASLREVAEGLFGVDAVAAD
                     WHADSALRARVRRLVRRGDGLMCGGYRRLAQLPAVISHERGRFASAAKRP"
     gene            49247..49528
                     /locus_tag="QBF46_000880"
     CDS             49247..49528
                     /locus_tag="QBF46_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6673102.1"
                     /translation="MRPAPLRPAAAPVATPAQPQRYLTNDEAADYLRLSPRTLEKQRV
                     IGGGPKFRKFGRRVMYAVADLNAWAETRSFETTSDPEYAEHHSADSRAR"
     gene            49555..50403
                     /locus_tag="QBF46_000881"
     CDS             49555..50403
                     /locus_tag="QBF46_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010280413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDQ6673103.1"
                     /translation="MSRPPGHALQQREQLDLFRALPGEDMAPRDAQDLMAYPFFSLAK
                     SRRTAPIDFRSGNITIRVEGTLEHGIATIWDADILIWAASQIVEAKDAGIQPSRRMQA
                     TPYEILRFIGRGKSLRDYQRFKAALDRLQSTTVATSIRETTGRRLHRFSWINEWKELA
                     DANGTPLGIELILPDWFYAGVLDAALVLTIDPTYFRLTGGIERWLYRLVRKHGGRQEY
                     GWQFDFRHLHRKSGSSTRFSDFAYDLRALVARQSLPGYELGIVRIPEDDMELLTFRPV
                     PQTARG"
     gene            50693..51331
                     /locus_tag="QBF46_000882"
     CDS             50693..51331
                     /locus_tag="QBF46_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6673104.1"
                     /translation="MIVALLNQKGGVGKTTLATHIAGELALRGQHVVLLDADPQGSSL
                     DWTQRRSQQGLPRLFSAVGLARETLHQEAPELAKRADHVIIDGPPRIAALARSALLAA
                     DRVLIPVQPSPYDVWASAEMVALIREAQMFRPQLAAAFVINRRVSTTVIGREARQALT
                     EQPLPALRSEVRQRIVFADSAAAGRLARETAPGSAAAQEITALVDELLRWPS"
     gene            51328..51615
                     /locus_tag="QBF46_000883"
     CDS             51328..51615
                     /locus_tag="QBF46_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HDQ6673105.1"
                     /translation="MSDNSNPPNGKRPAKRVGIGVRPPANPHAEAWIRQGDADALNKG
                     DLYTARLTLDITPAMRARIKLSAFTQGVTVADLLRGLLEREFPEHRRENTP"
     gene            51612..52157
                     /locus_tag="QBF46_000884"
     CDS             51612..52157
                     /locus_tag="QBF46_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2840 domain-containing protein"
                     /protein_id="HDQ6673106.1"
                     /translation="MTTSALSVAAPPAIAVTAAHTAPSSQPASAPLTRVALAYIEPRF
                     KLYLRFGEPARTLQLDRWRRCAMFMPGAILCRVRWQANDYGTIRWQLMVMQAATPLDA
                     VQRIPGVRPGARLLLHAEGDASVRAVLERIDGIEALGIAAIDVSPAYWRTLANRLAAH
                     SPLPEYTAERHAAWLAGRALP"
     gene            52154..52753
                     /locus_tag="QBF46_000885"
     CDS             52154..52753
                     /locus_tag="QBF46_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HDQ6673107.1"
                     /translation="MTAHPTAARTPEAAPLPRSRLRARLVLAGFAAAGLTALAWTVFA
                     QPLPRLIYNPSDSVPVGWYRVQPLDHRAAALPRPLSVGSIVLTRLPADAGVLAAQRGY
                     LPVHIPLLKRVGAVAPQHVCIVAGQVRIDGVPVAAALPADRLGRPLPSWQHCRALAEG
                     ELFLLSATNPASFDSRYFGPVSASAVIGVAHPVWLEIRP"
     gene            53202..55208
                     /locus_tag="QBF46_000886"
     CDS             53202..55208
                     /locus_tag="QBF46_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014603545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease and DUF3363
                     domain-containing protein"
                     /protein_id="HDQ6673108.1"
                     /translation="MTDRRDDDFRVRPSAPKNRGKGQGQSFVSKVLKQAGKASGGKSA
                     VRRPAAGGNGQHAGQRPGSRLGRGHTAARFAGAKLTPMSRRVTIKTLLVNQRNASPQS
                     LAKHLRYIERDGAGRDGEPGRAYGSQTDEADLDAFKERAADDRHHFRFIVSPEDGAEL
                     DDLRTYTRHLVNRMEADLGTRLDWVAVDHWNTDNPHTHLIVRGRDDTGKDLIIAGDYI
                     AHGFRHRAAELATEWLGPRTELEIQQTLGREVEQERWTSLDRTLQREAGDDGRVQIEQ
                     FNAPALQRQRLLLIGRLQRLQRLGLADETQPGTWAVHADAEKTLRALGERGDIIRTMQ
                     RAMSGQPRELAVFEPGQDDNESGRTIVGRVAAKGLADELRDRGYLVIDGVDGKAHYVA
                     LNARDELANYPTGAVVEVRGSAEVRAADKNIAALASDGLYRTDHHLAIEQGRVKPGRN
                     PQEVVAAHVRRLEALRRAGIVERVAEGLWKVPDDLAERGRQYDAQRLGGVAVEMKSHL
                     PIERQARVIGATWLDQQLIGGGKGLGDLGFGGDVKQAMQQRANFLAEQGLAEKRGQRV
                     ILARNLLATLRNRELTQAAKDIAAETGLEHRPVTDGQRLAGIYRRSVMLASGRYAMLD
                     NGKGFSLVPWKPVIEQRLGQQLAATVRGGGVSWEIGRQRGPTIG"
     gene            complement(55222..56022)
                     /locus_tag="QBF46_000887"
     CDS             complement(55222..56022)
                     /locus_tag="QBF46_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015840704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6673109.1"
                     /translation="MRTLERTRSDLAAFLRAHRERLSPADVGLPSGGRRRTPGLRREE
                     VAALAGVGLTWYTWLEQGRDIGVSATFLDNLARVLKLDAAERRHLFLLAHERPPAEPG
                     KTWCVLPPMVRRLMHDLPHPAFVLNLRWDVLGFNAPADALFGFGKHTPERRNLLWLLF
                     TDPALHARFHEWEAQAPLMLSSFRRDFARATQEADIHELVDELERVSPEFKAWWRTHD
                     VHASCNGVRKMVINGQAEPFEHTSLTVDEDRHLRLVVYAQQLKTADGE"
BASE COUNT        12930 a        15634 c        14456 g        13015 t
ORIGIN      
        1 tccgttcaaa attactatta aatatttccc agccattaaa agaatacttc tcgctcccag
       61 gatggttttg taataaaact tttttcatca cattccctgt tattacatac tttagaggcg
      121 ataattttat ccatgcaaaa aaaatatcca acgaattttc ttgatccgct ggatatttca
      181 gaatatgact cgaatagcac gaaagattca ctcgcttacg ctatcgcccc gcttccgact
      241 tcatctgctg gcggactttt tttcgcacta cgtttacgcg gtgcagcctt tttcttatca
      301 gcactgccac cactgcccgg agccacaatg ccgcgaaact gccgcaccgg cgtacgtttg
      361 gcttgatcaa taagctgata tagcgtcccc accagcggct gcataaagtc ctgatagcga
      421 cactgctttt cgctgatttg cgtcagcacc gattcccagt gcgcggtcat gtccggtcgc
      481 gtcgccatct ccggcagcga atggaatagc gcttttccgg cgtcggtgga gtggatatag
      541 cgcccttttt tggtcaggaa accacgcttg aacaacagtt caataatccc ggcacgcgtt
      601 gcctctgtcc ccagaccatc ggtcgcacgg aggatctttt tcagatcttt atcctgcaca
      661 aagcgcgcga tcccggtcat cgccgaaagc agtgttgcat cggtaaaatg gcgcggcggc
      721 tgggtttgcc gctctaccac ttcacctttt tcacacagca actcatcgcc tttcgccacc
      781 acaggcagtg gcgtgccgtc gttttcttca tcgcgctctt tgctgcctaa cagcgtgcgc
      841 cagcctgctt cagcaagaaa acgcgcttta gcgacaaatt tgcctttggc aatgtccagt
      901 tcgataacac acttgcggaa caccgcatcc gggcagaact gcatcagata ctgacgggca
      961 atcaggttat agaccttcgc ttcgttctcc atcaggttga tcgcagaact ccgtgcggtc
     1021 ggaatgatgg cgtggtgcgc atcgaccttt ttgtcatccc aacagcggtt gcgtatatct
     1081 ggatctacca ctggctgcgg caacagatcc ggtgcatgaa cactgatggc attcatcacc
     1141 gcgtggcgtc cggcaaaatg ttcttctggc aaatagcgac aatcagaacg cggataagtg
     1201 attagcttgt gcgtttcgta cagtttctgg cagatatcaa gcacgttctg cgcgctcaga
     1261 ccaaagcgtt ttgccgcttc aatctgcaac gccgaaagcg aaaacggcag cggcgcggat
     1321 tctgattccc gtttatcgtt atagctggtg acaatagccg gttgaccact aatgcggtta
     1381 accacatgct ccgccagtag acgatgtaac aagcgacctt cttcatcctg gtacggttca
     1441 cacgcttcgc tcggttgcca gatagcggta aaccgctcat cggcaggtgt cacgatatgt
     1501 gctttgactt caaagaagtc tttcgccacg aagttttcaa tctcttcatc gcggcgcacc
     1561 accagcccaa gcacgggcgt ctgcacgcgt cccacggaaa gtacgccctg ataaccggca
     1621 ttgcgaccga gaatagtata cgcacgggtc atattgatgc cgtacagcca gtcggcacgc
     1681 gctcgcgcca gcgcagaaac gcacagcggt acaaactcac tgttggaacg aagacggtcg
     1741 atcgcccgct caaccgcctg cgggttcagg tcgtttatca agcaacgctg cacctgctgg
     1801 cgcttttccg gtgccagttg cagatagtcc agcacttcat ccaccagcaa ttgcccttca
     1861 cgatccgggt ccccggcgtg aacgatttcg ctggcttcat gcaggaaccg tttgatgacg
     1921 ttaagttgtt tggtcacgga gggtcggggc tgtaattgcc acttttccgg gacaatcggc
     1981 aaatccgcaa gattccagcg cgcatagcgg ctgtcgtagg cgtctggctg cgcctgctca
     2041 agcaggtgac cgatacacca ggtcaccacc tgaccattac cgcactcgat aaagccatcg
     2101 cctttacggt gcggtttggg caggacatca gcaatggcgc gcgccagact cggtttttcg
     2161 gcaataaaca accgcattga attaacggat ctcaaccatg gcacgaccgc cgcgcgctgg
     2221 caccagttcg ccaatcgccg tcagttcaat gccaaactcg gcggctgtag ctttaacctc
     2281 attttctgct tccggcatga ccgccagcag caaaccgcca gaagtttgcg gatcgcacag
     2341 caaatcgcgc acttcacgcg gcatttcacc catcagatga ccgtagctgg caaagttacg
     2401 ttcggtgccg ccaggcactg cgcccaactt aatgtactct tcaacccccg gcaattttgg
     2461 gatcgcgtca tagtcaacgc gcgcctgcac acctgcgccc tgacacattt cgctcaaatg
     2521 gcccagcaga ccaaagcccg taacgtcggt catcgctttt acgccttcga tgttggcaaa
     2581 ggacgcgcct gcgatgttca tccggcacat cacttccgtc gctagtccct gatgttctgg
     2641 tttcagcagt gattttttct cagccgtggt aagaacgccg atccccagcg gtttcgtcag
     2701 gaacagtttg catccggctt gtgcggtact gtttttcttc acccgctcgg tcggtacgat
     2761 ccccgttacc gccagaccaa aaatcggctc cggcgcatcg atggagtgac cgccagccag
     2821 cgcaataccc gcctgacggc atgcatagcg tccaccttcg gttacttcgc gggcaatttc
     2881 cggggaaagt ttgttaatcg gccagccgag gatcgcaatc gccataatcg gtttgccccc
     2941 catcgcgaag atatcgctga tggcgttagt cgccgcaatg cggccaaaat cgaaaggatt
     3001 atcaacgatc ggcataaaga agtcggtggt actgataacg ctggtgccat tgcccagatc
     3061 gtacaccgcc gcatcgtcgc gggtctcatt acccacaagc aaattcggat caacaaactt
     3121 cgcctgctca ctgtgcagga tggtttccaa cacttttggg gaaattttac agccgcaacc
     3181 agctccgtgg ctgtattggg tcaaacgaat cgagttctcg ctcatggaca tctcctgtca
     3241 atgcaatccg ggtatggtaa ccctcattcc gtgaagtgat aagtgagagt gtctgaattc
     3301 ctgcgccttt gctcacaatc cagacagttt cgcgacaatt atcagaaata agttacaaac
     3361 ggcgtcgggt ccgggacgtt aatcgacgta gatgctttca actgcggcgt gccgaggtag
     3421 agaaaaccga caattttatc ctgctcacgg caaccgaatg cttcacgcac taccggactt
     3481 tcagttaatg cgccactgcg ccagatgccg ccaaatccct gggcaacggc tgccatttgc
     3541 atcgccatga ccgcgcatcc ggcagacatt tcctgttccc atcgcgggac tttatgattt
     3601 tcttcgcatt tcgccaccac cgtgatgatg agcggtgcgc ggaacggcgc attacgggct
     3661 ttgtcgatag ctttgtcatc actaccggca gcaatcgccc cctgttccag tacggcgctg
     3721 aaacgctcgc gcccttcccc ttcaatcaca aaaaaatgcc acggttgcat ggacttatgg
     3781 tccggcgcac gcatacccgc acgcaggatg ttttgcagtt gttcacccgt tggcgcgggt
     3841 tcagccaggc gggaggcgct acggcgattg atcaatagtt cgagtgcatc catttgatta
     3901 actcctgtcg tgatatttat tcacaaaatt aacacgagag tggattttgt tacagcacag
     3961 tccgcaattc ctgctgacaa gtgccggttg ggtcattacg ataaccacat ctatttcgcc
     4021 tgtgacaggt gtgaccttaa gttgggagaa tacatgcgaa ccctttggcg atttattgcc
     4081 ggatttttta aatggacgtg gcgtctgctg aatttcgtcc gtgaaatggt acttaacctg
     4141 ttctttattt tcctcgtact ggttggtgtg gggatctgga tgcaggtcag tggtgatgat
     4201 tcgaaagaaa cggccagtcg tggcgcactg ctgctggaca tttctggtgt gatcgtcgat
     4261 aaacccgaca gttctcagcg gtttagtaaa ttaagccgcc agctgcttgg tgccagttcc
     4321 gatcgtctgc aggaaaactc actgtttgat atcgtcaaca ccattcgcca ggcgaaggac
     4381 gaccgcaata tcaccggtat cgtgatggat ctgaaaaact tcgcaggcgg cgaccagccg
     4441 tctatgcagt acatcggcaa agccctgaaa gagtttcgtg acagcgggaa accggtttat
     4501 gccgtaggcg agaactacag ccaggggcaa tattatctcg ccagtttcgc caataaaatt
     4561 tggctgtcac cgcaaggcgt ggtggatttg cacggctttg ccacaaacgg tctgtactac
     4621 aaatcgttgc tggataagct gaaagtttcc acccatgtgt tccgcgtggg tacgtataaa
     4681 tctgccgttg aaccgtttat tcgtgatgac atgtcaccgg cagcccgcga agctgacagc
     4741 cgctggattg gtgagctgtg gcaaaactat ctgaatactg ttgccgctaa ccggcagatc
     4801 cctgctcagc aggtattccc tggcgcgcaa gggttgcttg agggtttaac caaaaccggt
     4861 ggcgataccg cgaaatatgc actggaaaac aagctggtcg atgcactggc atcgagtgcc
     4921 gaaatcgaaa aagctctgac caaagagttc ggctggagta agactgataa aaattatcgc
     4981 gccatcagtt attacgatta cgcattgaaa acgccggcag ataccggtga cagcatcggt
     5041 gtcgtcttcg ctaatggcgc aattatggat ggcgaggaaa ctcagggaaa tgttggcggt
     5101 gataccactg cggcacaaat ccgcgacgct cgccttgacc cgaaagtgaa agcgattgtc
     5161 ctgcgtgtta atagcccagg cggcagcgtt accgcgtctg aagtgattcg cgctgaactg
     5221 gcagcagccc gggcagcggg taagcctgtg gttgtatcga tgggcggcat ggcggcatct
     5281 ggtggttact ggatttccac gccagctaat tacattgtgg ctaaccccag caccctgacc
     5341 ggttctatcg gtatcttcgg cgtgatcacc accgtagaaa atagtctgga ttcgattggt
     5401 gttcataccg atggtgtctc aacttcaccg ctggcagatg tttctatcac cagggcactg
     5461 ccgccggaag cacagcagat gatgcagtta agcattgaga atggctataa acgctttatc
     5521 acgctggttg ctgatgcgcg tcattcgacg ccggagcaga ttgataaaat cgcccagggc
     5581 cacgtctgga ccggtcagga tgcaaaagct aacgggctgg tcgatagcct cggggatttc
     5641 gatgatgcag ttgccaaagc agcagagctg gcaaaagtga aacagtggca tctggaatac
     5701 tacgttgatg aaccgacctt cttcgacaaa gtgatggaca acatgtctgg ttctgtccgg
     5761 gcaatgttgc cagatgcgtt ccaggccatg ttacctgcac cgctggcctc ggtagcctct
     5821 actgttaaaa gtgaaagcga caagctggcc gcgtttaacg acccacaaaa ccgttatgcg
     5881 ttttgcctga cctgcgccaa cgtgcgttaa gtcttgtact gagtggccga cagatcgtcg
     5941 gccacattat tttttacgtc gacgaatcct cttcccgctg tctcgcccca tatttcctta
     6001 tccgcatagt atcaggtgcg ctcctcctgc ctcacgtata tacttttgct ctttcgatat
     6061 cattcatatc aatatcatgc aaaagaaatc aatttacgtc gcctacacgg gcgggaccat
     6121 cgggatgcag cgttccgagc agggttatat tccggtgtca ggtcatctac aacgccaact
     6181 ggcgctgatg ccggaattcc atcgcccgga gatgccagat ttcaccattc atgaatatac
     6241 gccgttgatg gattcttctg atatgacgcc agaagactgg cagcatattg ctgaagatat
     6301 taaagcgcac tatgacgact atgatggttt tgtcattctg cacggcaccg atacgatggc
     6361 atataccgcc tctgcgctgt cgttcatgct cgagaatctc ggtaaaccag tcattgtgac
     6421 agggtcacaa atcccgctag ctgagttacg ctctgacgga caaattaatc tgctgaatgc
     6481 gttgtacgtt gcggcgaatt atccgatcaa cgaagtaacg ctctttttca ataaccgatt
     6541 gtatcgcggc aaccgcacta ccaaagccca tgccgatggt tttgatgcgt ttgcctctcc
     6601 aaaccttcct ccgttactgg aagcaggtat ccatattcgt cgtttaaata cgccacccgc
     6661 cccgcacggt gaaggggaat taatcgttca tccaatcacc ccacaaccaa ttggcgtagt
     6721 gacgatttat ccagggattt ctgctgacgt cgtgcgcaat tttctgcgcc aaccggtgaa
     6781 agcattgatt ttgcgctcct atggcgtggg taatgcgcca caaaacaaag ccttcctgca
     6841 ggaattacat gaagccagcg atcgcggtat tgtggtggtc aacctgacac aatgtatgtc
     6901 cggtaaagtg aacatgggtg gttatgccac cggtaacgcc ctcgcccatg ccggcgttat
     6961 tggcggtgca gatatgactg tagaagccac gctaaccaaa ctgcattacc tgctgagcca
     7021 ggaactggat actgaaacca ttcgcaaggc catgagccaa aacctgcgcg gcgaactgac
     7081 gccggatgat taaggagact gcaatgcccc ctcgcgccct gttactggtc gatttacaaa
     7141 atgatttctg tgctggtggc gcgctcgccg tgccggaagg tgacagtacg gtggatgtcg
     7201 ctaaccgcct gattgactgg tgccagtcgc gcggtgaagc ggttatcgcc agtcaggact
     7261 ggcacccggc gaatcacggc agttttgcca gtcagcacgg tgtagagcct tatacgccag
     7321 gccaactcga cggtttgcca caaaccttct ggccagatca ctgtgtgcag aacagtgaag
     7381 gcgcacaatt acatccgtta ctgaaccaaa aagcgatcgc agcggtgttc cataaaggcg
     7441 aaaatccttt agttgacagt tacagtgcct tttttgataa cggccgtcgg cagaaaacct
     7501 ctctcgatga ctggttacgc gatcatgaaa tcgatgaatt gatcgttatg ggcctggcta
     7561 ctgactattg cgtgaagttt accgtgctgg acgcgttaca gttaggttat aaggtaaacg
     7621 tgattaccga tggctgtcgt ggcgtgaata tccagcccca ggacagtgca cacgcgttta
     7681 tggagatgtc agcagctggg gcaacgctat atacgctggc agactgggaa gagacgcagg
     7741 ggtaatttta cgctggccta caattctgta ctggcattgt aggccaaata aaacacgtca
     7801 gtggtacatc tggcaattga tgccatcaac gaaagattta attcacctca gaaatctctt
     7861 ccagcgacac cttccgcgtt tcgatgccaa aaatggagag aaccagcgca cagagcacta
     7921 acatgacacc aagcaccata aacaccgtga tcgacccata atgtgttaat aatgccgcaa
     7981 cgccataggg cgtgaaaact gcgacgatcc gtccgacggc attaacgaaa cccgaaccgc
     8041 gcatgcgtaa atgcgttggc caaagctctg ggatataaac cgccgacgcg aagcaaacat
     8101 acatgtataa aaagaagatc atcaccaggc catagattaa aatcgcccac tctgtagtct
     8161 gaattgaata gatatagcct aacacggcaa taataataag taaggtggag ccaaataacc
     8221 gacgaggaaa atgatcaata attaatgccg caataaatat tcctaccgga gcgccaatca
     8281 taataacagc ggtcattaat attgatttat cgacatcaat gccggagtta acaaatatgg
     8341 tcggtatcca tacggtgatg gtataaagcg agatgttcat tgcaattaaa acagtaatcg
     8401 cgactaatgt acgtcgtaac atttcacctt taaacaggag ccagaaagta cccttgatta
     8461 ctttaacctt gctgttgctc tgatacgaag taagcggggg taaacgaata ctcttctctc
     8521 tttcaatttg ctgctctact tcatgaagtt ggctttctgc accggcgatt tgccctttcc
     8581 ctgccagcca tcgtggcgac tcgataaagt atttacctga gagaaaccag gctaacagta
     8641 tgccaatacc acccagcaga aacattattc gccaactaaa aaaagcgata accaccacgc
     8701 ctatcgccgc agacagcatg ggcgaccagt taccaacaaa tgagagccgc gcggaccatt
     8761 ttccacgcac cgtcgcgggg ataaactccg taaatgaggc atagccaacc ataatcagcg
     8821 cccccattcc tgttcccatc aggaagcgaa agaagatgag ccagtacata tcagggacaa
     8881 aagcggcccc tgttgcagca ataccgacga tgagaagatt tatgcgaaac gccctgcgcc
     8941 gcccaaagta gtcaccaata aacccaccag taagtgagcc gatgaaataa ccaaacatta
     9001 atgccgaggt aaaggcggca ttgaggaaat tatttgacca tccattgctt accagctttg
     9061 ctaagacgac attaccggag taactcaaaa accctgttaa taacagacta aagcttataa
     9121 taccaaatat acgataatga aaccgggcca aaggcaagcg gtccagtctt gcgccaattt
     9181 gatcatattg ttccattagg tgcctcggat ttgctaaaaa ggtagcaatt gcggcaaatt
     9241 cacttgccgc atctttatca tcattttatt tcaaggcgca ggaacaaata atattgacaa
     9301 cgtgtgctta aattaccaca ccagctggat attattgtct ttgcaaaatg ctatccactc
     9361 tgcacccggt gatttatcag ttattatgta attaatatga ctaaaatcag ctaactggac
     9421 aaaagctttg cgatcaaact tagagtgatc aaccaataac gcaacttctg tcgcctgacg
     9481 gatcattgtc tttttgattt cagcttccgc ttcattagag tccagcgcgc cgctgttaat
     9541 atcaagacct ttacaactca ttaccatgat atcgacatga tagcggctga tgatctcttt
     9601 agtaattctt ccttgcagtg aaagtgtatt tttgtttagt tccccacccg ttgaaacgac
     9661 tttaatttct gactgagcca gaacatgaat cgcttccgcc gagttggtta gcaacgtcag
     9721 gtcactacgg tcctgtaaca atttgagcaa ttccattacg gtactactgg aatcggctgc
     9781 catggtggtt ttattgtcga taaagggtag tgccttgcgt gcaataagct gcttctcttc
     9841 ataaaacgat gaagcgcgct tataaaaatg gatattctcc gtcaacatcg ctgtatttaa
     9901 aacagcacca ccataggttc tggtcaaaaa gccttcatct tccagcttct caagatcgcg
     9961 gcgaatggtt tcttcggtta cctgaaaaat cccactcaaa tttgagactg tcactttttt
    10021 atcgttggca accatttgct taattgcctg aatcctgtct tttgccgcca cgattacacc
    10081 cctgtatctt tttacatcac attagcgcga ttatcgcata accgatgttt actttcaaaa
    10141 caacctattt gaatcacaga ttttcatcac agttttcaca gaaacagagg tgaatcgtgt
    10201 tgagtattta acgctccagg gcctctgcca tttccctcat caatgttgcg tctgcatccg
    10261 ataagttgat aatcagtgcc gcgacatttt cgcgtacctg ttccggtgca gtagccccac
    10321 taagaatgga gattaaatca ctctgtttta atatccacga cagtgccaga gtgggaattg
    10381 tgcactgata acgagcacaa agtggctgcc actgttcaag catatcaatc actttcagca
    10441 tgttttcacg ctggaactag actttatttg cccgagcgcc gcccggaacg taatcacgag
    10501 tgatggtgcc ggtcaacaat ccctgctcta gcggggaata aacctgaacc acaatgccat
    10561 tatcacgaca tagcggcagc agttcgtttt ccattgcccg gtcgaggata ctgtatttcg
    10621 cctgaataat atccagttca ccatattgca gatactcgcg gatatggtca gcatcgacgt
    10681 tagcagcgcc tatagcgcga attttccctt cagccttcaa ctcattaagc actgcgacag
    10741 tttcagcgat cggcgtaaaa aatggcggca ccgactgcca gtgcgtcatg tagatatcga
    10801 tgtaatcaat acccagacgt tgcaagctgg cttctacctc ttcgcggata gattccgggg
    10861 aaaggttttt atacaactgc cgatcgccaa ctttgttgaa taaacttcct tttcgttccc
    10921 agacaatgcc gcatttggtt tctactacaa cctgttcacg gggcagtttt tttaacgcct
    10981 gaccgacgat aacttcacta ttgccaaagt tatatcctgg cgcagtatca atcagattaa
    11041 tgccgcaacg atgggcttca agaatcgtat caatacatat ttgccgatcg agatcgccat
    11101 tccatgcagg accgccgcca atggcccatg tccccaaccc cattcgcgaa agcgtaatat
    11161 ccgttgtgcc taaaggtatc tttttcattt gccttcatta tccttcgtat tcttccagca
    11221 attgttcgac cagctttctg ttttttacgc cggtggtggc accgacgctt agaaccgaga
    11281 tagccgccgt tgcattggca aagcgtgcgc attcacgcag atttttgcct tctaacagtg
    11341 ccgcaataaa acctgaagca aagttatcgc ccgcgccaat ggtgtcgatg gcggttattc
    11401 ctgcgaccgc cggcaccttc atcgtcatgt caccacgctt gataaagcag ccgtctttac
    11461 ccgttttaat caccaccgtt tttacgccgc acgcaagaaa gcagtcagca atttcatcca
    11521 atgtctcttt cccggtgagt aattttgcct cggcataatt aggaaacaga taatcgacat
    11581 aactcaatgc ttcgcaaata tcatccagcg tttcattcaa ccgcggtttg atcatatcgg
    11641 cacagataat catctgccgg gttttagctt gcgtaaaaat ttctgttagc gctttaccat
    11701 ccaatagagg actgttgaaa atactggcca gagataataa tttcgcctga gaaaaccgcg
    11761 aaaaatcaac atcgtcaata ttcaatttcc acagactgcc gttacggttt gtgacaaacg
    11821 tccgctcgcc atcctccgtc accagtccaa cgttaataga ggtatctatg ctgacgtcct
    11881 gcttcaggct ttgaatatca atattctctt tgcagcaatg gtcgagaata aactgtcctg
    11941 cggcatcttt accaatacgg ctcattaatg ctgtacgatg gcccagacga gaaataattg
    12001 ttgcttcatt tattgcatca cccccggtgg tcattgcgat tctttaaaga ggataagaat
    12061 ccacatcaaa gatattttta ctgaccggtt gcaatggaat atcaacaata gcggcaccta
    12121 tacaaataac gtcgagatta tccatatcat tattccgctt tgccatcaga accgaacagt
    12181 ttaatttttt ccaatgcccg ttcttttaca gctttacgta cttcacgttc taaatgcagg
    12241 aaaggctgat cctggttttc tttcactgcg accattgcgg cctgacagag ctccgtatga
    12301 atgttgattt ttgcgatacc caacgaaatt gcagttttaa tgtcggcatc actgatcccc
    12361 gatgcaccgt gcaaaaccag cggaacagaa acggcatcgc gtacgcgttt gacgacctca
    12421 aagttcaatt gtggctctga cgtataaacc ccatgctggt tgccgatggc gacagccaat
    12481 gagtcacagc ccgtacgttc aacaaattca gccgcctggt caggatcggt atagtgatag
    12541 cccgccagcg cttcctcata aacggtttca ttaccgacat gccctaattc cgcctctacc
    12601 ggaataccca gcgatggaag aaatcgacag cctctttggt taaacgaata ttttcttcga
    12661 aatcaaacgc ggaagcatca cgcattaatg aattcatacc atgagtccag gcgttatgaa
    12721 taatctccat actccgacca tgatcccaat gagttattac cggcaccgtt gctttttgtg
    12781 ccattgatac catcatgtga gagaaatctt caaatgaggt gttgccgaca aaacctgtgc
    12841 caaaagaaat aataaccggt gatttcgctt cttcggctgc gtcgataacg cccatcaaca
    12901 tttctgcatt ccatacgtta aaatgggcaa ttgcataatg tttatttgtg gcatcgtttt
    12961 cccaatatct gatatctgcg agcatgttct gttctcccgt attaattaat cgctaatttt
    13021 aataacgcct ttaataatgt cgcgtttgtt gttaactgac tcttcaaatg cctgttgtac
    13081 atcccgataa tcgtaaatat gcgtcaccat cgatttcaca tcgaatcgcc ctgaagaaat
    13141 agcttcaatc gtgaccggat aacgattggc atagcggaat accatctgga tagtgacttc
    13201 gcgattgatt ttgaggaaat tgattgccga atcgcccggt acagtaccaa caatcataat
    13261 tttaccgccg cgcattacca gataaggtgc ctgtttaatg gtgaccgcag aacccgctgt
    13321 ttcgaaaaca atatctgcgc ccatgtcttc ggtaaattgc tgacagcgtg caatagtgtc
    13381 ttcttttgcg ccgttaataa ccactgtcgc accaagttgt tccgccatta tcagacgttt
    13441 ttccagcaca tcaacgacgg caatttccgt tgcgcccagg catttgcacg cttgcaacgt
    13501 catcaaacca atacaacctg ctcccagaat aattatcttc ttacccggtt taacatctgc
    13561 cagcatcgcg gcatgcatcc cgactgcggc aggctccacc agcgcccctt ccatcgtgtc
    13621 catattgtct ggcagtttgt aagtaaagct ctccggatga cacagatagt gcgttaatgc
    13681 gccgcggtag ttggggtgtg tcgccataaa atcaacgtcc gggcagatgt tatatttgcc
    13741 ttccagacag taacgacagt gaccgcaagg aacgccaggt tcgatattta cccgatcccc
    13801 cggtttaaat ttgcgcacgc ggcttcccac agccacaacc gtcccggcgc attcatgacc
    13861 caggccaatt tcttgatttg ggtctttagg cggaataaac gggcctgatt caaaaccatg
    13921 tacatctgaa ccacaaatac cgacatattc tactttaatc aaaacttcat cttctttagg
    13981 cactggtatt tctgctgaaa taattttcat tgtgcccggc acctgcaata ttgcttttga
    14041 atttttcatt ttgaatccac ccgtattatt tattggctac tgcatcaata cttaaacttt
    14101 caacggagac acctttggtt tcaattccaa tggtggcgat agcaattgcg acaacaattg
    14161 aaacagcccc cagaagaata aagactcccg ttacgccata actactgagc agcactgcaa
    14221 cggcataagg tgcggcaata ctactgattc gccctaccgc attcgccaga ccggagccac
    14281 ggagtttggc ctctgtcggc cagatttcag ggacatacac tgtcgaggca tagcaaacgt
    14341 acatatagac gaaagtaatc aggaagaaac caatcaacgt tatgagcaac atactggttt
    14401 gcagtgaata gatatatccg agcaccgcaa tcaggattaa tagccccaca cccattgttt
    14461 tacgcggaat tttatccatc accagcatgg caataaaaat accaaatggc gcgccaaaca
    14521 tactcatggt atttaaaaca atcgagtctt ttaaattaat cccctgggtc atgaatattg
    14581 ttggcaacca gttaattagt gtgtactgca caacgttcat ggcaatcagc acacaagagc
    14641 ctaatatcac gcgtttcagt aatactcctg tcagtaaggc tgaatacggc accgcttgtg
    14701 gcgctttacc gtcatcagca ataaccacag gcggcaaagg tttacccgtc tggcgtatga
    14761 cgccttcttc tatactccgc atgactttct cggcttcctg ataccgtccg cgcgattcca
    14821 gccagcgcgg ggattcagga aagtagcgcc aggccagcgc cgtagcgata agcgacaata
    14881 ttgcagggat aagcagttgt acccgccagt tccactctgc actaatcagc ggcgtgagcc
    14941 ccatcgctat caatgaacag agcggatatg accagttgcc aataaaggaa acccgacttg
    15001 accacgttcc acgatttcta ccgggcatat attcggtgaa accagcaaac agcgtaacca
    15061 gtaaagctcc cagcccaact cccatcacaa aacggcaggc aataaggaaa tccatattcg
    15121 gtgagaaagc accgacaacc atcgaggcaa tatgaatggc ctcatagagg atgaacgcat
    15181 ttctgcgacc ggttttgtcg ccaatgatgc cgcccaccag cgcaccgaga aacattccgg
    15241 cggtcgtgat tgctgagaat gtggctgtgg tggaattatc tgtccagccc aacgctttca
    15301 gctgcgcgag gatcaagcca ccaacggcat tactccagca gacaagcaag ccaaacgcga
    15361 cgatggcaaa cattgacgaa tgccagcggc aatccggtaa gcgatccagc cgtgcaccac
    15421 aatgcggttt tgttatctgt tccatgcgat aacgttcctt caggagagtg gttattcatc
    15481 aaagtcgtaa gtcatgatca ctttgattgc ggttttatcg accatcgcat caaacccttc
    15541 gcgccattgc gacaggccga tacgatgcgt gatcatcggt ttgactttga tagcgccgct
    15601 ggctaatagc cggatagcgt tacgccatga ggtggagtca taggccatat gcccaatgat
    15661 gcttttattc caggcggtaa tgtcattaat cgagaaatca agaggtttga agcccattcc
    15721 aacgcgtacc acttccccat tcgggcggag catttcgatg gcttgtttca gtgcgatatt
    15781 ggcaccggag cattcaatca ccagtcccag attatctttg ccacaaattt gctggcagcg
    15841 cgccaccaca tcttcggtag aaccatttac tactgccgtc gcccccagtt cttttgcaac
    15901 cgggaagcgg accgccacat cttcttgcag accaacgacg acgatattta ccgcccccat
    15961 aatccgcgcc atttgtacgg aaaacagccc gagtgggcca gtgccgatga cgacaacatc
    16021 ctgaccagga aggaatttcg attgctgcgc gatggattta taggcattac agatagggtc
    16081 aagtacggct gcgtcctcat aatcaacacc atcagggatt tcccacaacg catgacgatg
    16141 aattttgaga atttcaccag gaaccagaca atatttggaa aaaccaccgc cccaggtatt
    16201 gttatccaga ccaaggttta ctttttctgt acaacacaga aaatcaccct gttcacaggt
    16261 cggacaaacg ccgcaaacgt gaccgctgtt atccgacacg acgcgttgcc ccactttcca
    16321 gtctttgact ttttcaccaa cctgcgcaat acaacctgca aactcatggc cgcggataga
    16381 gttaaactca tcagaaccac tatcgacatt gtagtgcttc atgtctgcgc cgcagattgc
    16441 cgcggcttta atttcaatca ctacgtcttc cgggccacaa atgggttgtg ggacatcaat
    16501 catcttgtag ccgccaaagg ccttgccaaa ccgagccagt gctttcattg cgtcatcctc
    16561 tgtgttttgt tttgcttaga tggccatcat acttacccac atgatttttg aggtcaacat
    16621 taaatctgta aaacacagat aatgatctgc tttttacaac ttagatcaca attacgcaga
    16681 aaatggctgg gtctgttaca ggttggtgga aggcgggacg caaaaagagc aaattcgaga
    16741 gggattaccg gccaaatagg tctgagactt aagttcagga gaagtcattc aggcgtgaac
    16801 gcgactgcaa gagatcggac tgggacttag ccaggtcggg tcgagataat tgagacgagt
    16861 cgtggggtca ttgttggcta tccccttcat agccgcggca aaaaggggat gaaccactta
    16921 cttaaacata gcaataggct ttgctgagat accaaaatct tcttttaact gctgtttgct
    16981 cttcatgacc atctgaccat ttgtgtcaat tgtcatatgc tgtgcttcgg tattatggcg
    17041 agcttgccac agcatcacca gttgcaggca gttcgctttt tgttcctcgg tcaacgcaac
    17101 gccatcgggc catttcccca gttcaacggc ggtcgacaaa cgctggtata cctcaggcgt
    17161 catgctgttg ataatgtcat caagattcat aatttttccg ctcctgtgga ataagttgct
    17221 gaatcgtttt ttcaaccgtt gatttcttcg ccgttttcgc catcggtaaa gcgtaaagag
    17281 gcagagttaa cacaataacg ttcgcccgtt ggctgcggcc cgtcagggaa gacatgcccc
    17341 agatgggcat cacagttacc gcaacgaatt tctatgcgct gcattccatg tgacaagtct
    17401 ttgatataac gaatggattc ttcactcacc ggttcgtaga aactgggcca accacagccg
    17461 gaatcatact tggtttggga atgaaacagc ggggcatcgc agatcaaaca gtgatatacg
    17521 ccgtcacgct tgttatgcag taaacgaccc gtaaatggcg gctctgtccc atgattctgc
    17581 gtcacgtaaa actgcatctc ggacaaattt tttttcagtt cttctgccga aggtttatta
    17641 gccatttgct cacatctcac tttaatcgtg ctcacattac gtgactgatt ctaacaaaac
    17701 attaacacca actggcaaaa ttttgtccta aacttgatct cgacgaaatg gctgcaccta
    17761 aatcgtgatg aaaatcacat ttttatcgta attgcccttt aaaattcggg gcgccgaccc
    17821 catgtggtct caagcccaaa ggaagagtga ggcgagtcag tcgcgtaatg cttaggcaca
    17881 ggattgattt gtcgcaatga ttgacacgat tccgcttgac gctgcgtaag gtttttgtaa
    17941 ttttacaggc aaccttttat tcactaacaa atagctggtg gaatatatga ctatcaaagt
    18001 aggtatcaac ggttttggcc gtatcggtcg cattgttttc cgtgctgctc agaaacgttc
    18061 tgacatcgag atcgttgcaa tcaacgacct gttagacgct gattacatgg catacatgct
    18121 gaaatatgac tccactcacg gccgtttcga cggtaccgtt gaagtgaaag acggtcatct
    18181 gatcgttaac ggtaaaaaaa tccgtgttac cgctgaacgt gatccggcta acctgaaatg
    18241 ggacgaagtt ggtgttgacg ttgtcgctga agcaactggt ctgttcctga ctgacgaaac
    18301 tgctcgtaaa cacatcaccg ctggtgcgaa gaaagtggtt atgactggtc cgtctaaaga
    18361 caacactccg atgttcgtta aaggcgctaa cttcgacaaa tatgctggcc aggacatcgt
    18421 ttccaacgct tcctgcacca ccaactgcct ggctccgctg gctaaagtta tcaacgataa
    18481 cttcggcatc atcgaaggtc tgatgaccac cgttcacgct actaccgcta ctcagaaaac
    18541 cgttgatggc ccgtctcaca aagactggcg cggcggccgc ggcgcttccc agaacatcat
    18601 cccgtcctct accggtgctg ctaaagctgt aggtaaagta ctgccagaac tgaatggcaa
    18661 actgactggt atggcgttcc gcgttccgac cccgaacgta tccgtagttg acctgaccgt
    18721 tcgtctggaa aaagctgcaa cttacgagca gatcaaagct gccgttaaag ctgctgctga
    18781 aggcgaaatg aaaggcgttc tgggctacac cgaagatgac gtagtatcta ccgatttcaa
    18841 cggcgaagtt tgcacttccg tgttcgatgc taaagctggt atcgctctga acgacaactt
    18901 cgtgaaactg gtatcctggt acgacaacga aaccggttac tccaacaaag ttctggacct
    18961 gatcgctcac atctccaaat aagttgagat gacactgtga tctaaaaaga gcgacttcgg
    19021 tcgctctttt ttttacctga taaaatgaag ttaaaggact gcgtcatgat taagaaaatt
    19081 tttgcccttc cggtcatcga acaaatctcc cctgtcctct cccgtcgtaa actggatgaa
    19141 ctggacctca ttgtggtcga tcatccccag gtaaaagcct cttttgcatt acagggcgca
    19201 caccttctct cgtggaaacc tgcgggtgaa gaagaagttc tgtggttgag caacaacaca
    19261 ccgttcaaaa atggcgtcgc tattcgcggt ggcgtaccgg tttgctggcc gtggtttggt
    19321 ccggcggcac aacaaggtct gcctgcgcac ggttttgccc gcaacctgcc gtggacgctg
    19381 aaatcacatc atgaagatgc tgatggcgta gcgctgactt ttgaattgac gcaaagcgaa
    19441 gagacgaaaa aattctggcc gcacgacttt acgctgttag cgcatttccg cgtgggtaaa
    19501 acttgtgaaa tcgatcttga atcacatggc gaatttgaaa ccacctctgc cctgcatacc
    19561 tactttaacg tgggtgatat cgctaaggta agcgtcagtg ggctgggcga tcgcttcatt
    19621 gataaagtga atgacgcgaa agaagatgta ctgaccgatg gtattcagac cttccctgac
    19681 cgtaccgatc gcgtgtatct gaatccacaa gattgcagcg tgattaatga tgaagcgctg
    19741 aatcgtatta tcgccgtagg ccaccagcat catctgaacg ttgtcggctg gaacccggga
    19801 ccggcgcttt caattagcat gggtgatatg ccggatgatg gctacaaaac atttgtttgt
    19861 gtagaaacgg cttacgcttc agaaacgcaa aaagtgacca aagagaaacc tgcacatctg
    19921 gcgcaatcca ttcgcgttgc gaaacgttaa tttacgttaa tgttgtgtgc cgggtgcgat
    19981 gcatccggca cacaacatca caccatatcc agcgcagttt ttccttttgg tgccggatat
    20041 gccttatcca gcatagctaa ttccgctgaa gaaagttcga cctcaagcac agccgcattt
    20101 tgttggacat gggcaatcgt ggccgctttt ggaatcgcca tcacaccctg atgactgatc
    20161 acccacgcca acaatacttg tgccgcgctg atattgtgag catgtgcaat ttcgttgact
    20221 accgcgtttt ttaacagtcc attgcgcaac cgcccggcct gggctaacgg actgtaagcc
    20281 atcaccggca tctgctgttg ctggcaccag gggagtagat cgtactcaat tcctcgtgaa
    20341 ccgagatggt aaagcacctg attagtggca cactgatttc cccccggcag ctgccagagt
    20401 tcctgcatat cagcataatc aaggttagaa acgccccagc ggcggatttt tccctgggcg
    20461 atcaattttt ccatcgctgc gacagtctct tcaaaagcga aactgccaga ccagtgtaat
    20521 aagtaaagat cgagataatc agtattgaga cggcgtaaac tggcttcgca tgcatttatc
    20581 gctttttgcc cgccagcatt ccacggatag actttagaga cgagaaagac cttctctcgc
    20641 agaccggtta atgcttcccc aaccaccttt tcggcaccgc catcggcata catttcggcg
    20701 gtatcaatga gggttaaacc gagttcaatg cccgcgcgta gtgcagcaac ttctgttttg
    20761 cgctgactgg catcttcgcc catataccat gttccctgcc ctacggctgg cagtgagaca
    20821 tcgccactaa attgaatcat tttttgttgc attgtttcct cccaggtatt gcaccaccgt
    20881 aatgcaaaac agggcgtgac gccctgtttt tatgcacaaa atgccctgga aagatgcatt
    20941 atcagaattt gtaggtgatc ccggtagaaa tcaggccagt ccaggattta tccaccatcg
    21001 ggctgtcagt aacttcatca gacagacggg tgtagcgtgc ggtaccgtaa acactccagt
    21061 cgccgaggaa gttgtagctg gcgctcagct ccaggtaagg gctccagctg tcgttcgggt
    21121 tatagccacg cagaccgctg cgagcggact ctttgcgcga tacgccataa tagtattcgt
    21181 tctggttttc gctgttccac tgcacaccaa tacccggagt cacggtcagg ccaccgttgg
    21241 tgtaacgata caaccaggcc atatcccaga cgatgccgtt gctgttatcc agggtatcgc
    21301 cagccagggt ggtacgcagg taaccgtact gggtaaagtg agcataagac agaccagcca
    21361 tcatggtgct cttacggtca tccaggtgac gcatttggtg atcgccactg tctttcgctt
    21421 tgaagtaaag cggcgaccag taagcggtaa ttgaaagttt atccgttgag tcattccaca
    21481 ggtagtaacc accacctaag ccacggaacc agaagttatc gccttcatag ttgattaccg
    21541 gtactgggta aacatcggta tcgtaatctt tatatgggtg ttcaacgaca cctacgcctg
    21601 cgcccaggga aaatttacct tcagcgtgcg ctacgcctgc agacgttgcg ataagcactc
    21661 caagtgccag aagtttgagt ttggtcacaa ttaatcattc cttaaacaaa tgtttagcgg
    21721 gcgacaaagt ttacccgtca atacatcgaa acccaacctt tttacgtttt cattttttaa
    21781 agtaactgtt taattttcct gacgcggatg acaccgcgct tacagccaaa tgaatttagc
    21841 gttactggcg agcctggtct ttacattaat tatgcaaaat ttatggatga gttgttgata
    21901 tgccattgaa attaagaaag ccgtgcaggc aagttttcca tttgccatct acgcttaatt
    21961 ttgaaggtgt atcaccgggc acgttgttct catcgtcgat aaaatggcat gagagttgct
    22021 gtgttttagc aagagacgtc gttcagttta cctcttccgg gagcctctac tattcatatg
    22081 aacggctctt aacctgtgct aaaaaacgaa aggacggcat accatgaata tattcgatca
    22141 ctatcgccag cgatatgaag ctgccaagga cgaagagttc acactgcagg agtttcttac
    22201 cacttgtcgg caagatcgca gtgcttatgc caacgcggct gagcggctat tgatggctat
    22261 cggtgagcct gtcatggtcg atacagccca ggaacccaga ctttctcgac tcttttctaa
    22321 ccgggtcatt gcacgttatc cggcgtttga agagttttac ggcatggaag acgcgattga
    22381 acagattgtc tcttatctga aacacgcggc tcaggggctg gaagagaaga aacaaatcct
    22441 gtatctgctg gggcctgtgg gtgggggtaa atcatcgctt gctgagcgac tgaaatcatt
    22501 aatgcagctc gtaccgattt atgtattgag cgcgaacggt gagcgtagcc cggtcaacga
    22561 tcatccgttc tgtcttttca atccgcagga agatgcgcag attctggaaa aagagtatgg
    22621 cattcctcgc cgttatctcg gcaccatcat gtcgccgtgg gcggcaaaac gcctgcatga
    22681 atttggtggc gatatcacta agttccgggt agtgaaggtc tggccgtcaa ttctgcaaca
    22741 aattgctatc gccaaaacgg aacccggcga tgagaacaac caggacatct ccgcgctggt
    22801 tgggaaagtc gatattcgta aactcgaaca ctacgcgcag aatgacccgg acgcctacgg
    22861 ctattccggt gcgctgtgcc gcgccaatca ggggatcatg gaattcgttg agatgtttaa
    22921 agcaccgatt aaagtgctgc atcccttgtt aaccgccact caggaaggta actacaacgg
    22981 gacggaaggt atctccgccc tgccgttcaa cgggattatt ctcgcacact cgaacgagtc
    23041 cgaatgggtc actttccgta ataacaaaaa caacgaagcc ttcctcgatc gtgtttacat
    23101 cgtgaaggtg ccgtattgct tgcgcatttc cgaagagatc aaaatctacg agaaattgct
    23161 taatcacagt gaattgactc acgccccatg cgcccctggc acgctcgaaa cactgtcacg
    23221 tttttccatt ctttcgcgcc tgaaagagcc agaaaactcc agcatttatt caaagatgcg
    23281 ggtttatgat ggcgaaagtc tgaaagacac cgatcccaaa gccaagtcgt atcaggaata
    23341 tcgtgactac gccggtgtcg atgaagggat gaacggtctg tcgacgcgtt ttgcgtttaa
    23401 gatcctctcc cgcgtgttca acttcgatca tgtagaagtg gcagcaaacc cggtccatct
    23461 gttctacgtc ctggaacagc agattgagcg cgagcagttc ccacaagagc aggcagaacg
    23521 ctatctggag ttcctgaaag gttatctgat cccgaaatat gccgagttta tcggcaaaga
    23581 gatccagacg gcctaccttg aatcctattc cgaatatggg caaaacattt tcgaccgtta
    23641 tgttacctac gcggatttct ggattcagga tcaggagtat cgcgatccgg ataccgggca
    23701 gctgtttgac cgcgagtctc ttaacgccga gctggagaaa atcgagaaac cggcggggat
    23761 cagtaatcca aaagatttcc gcaacgagat tgttaacttc gtactgcgcg ccagagcgaa
    23821 taacagcgga cgcaatccga actggaccag ctatgaaaaa ctgcgcacgg tcatcgagaa
    23881 gaaaatgttc tccaataccg aggagctgtt gccggttatc tcgtttaacg ccaaaacgtc
    23941 aaccgacgag cagaagaaac acgacgactt tgtcgaccgt atgatggaaa aaggctacac
    24001 ccgtaaacag gtgcgtttac tgtgcgaatg gtatttgcgc gtacgtaaat cgtcttaaca
    24061 accctggccc ggtacacatg ttaccgggcc tacaacgaca gcgaaccgtg ggcctgagaa
    24121 gcggcaacac aggcgtagca tacagttggc aaatgtagta cgggaggcat atgacctggt
    24181 ttattgaccg gcgtctgaac ggcaaaaaca aaagcatggt gaatcgccag cgttttttac
    24241 gccgttataa agcgcaaatt aaacagtcga tctccgaggc cattaataag cgttcggtga
    24301 ctgacgtcga cagcggcgaa tccgtatcca ttcccacgga agatattagc gaaccgatgt
    24361 ttcatcaggg gcgtggcggt ctgcgccacc gcgtgcatcc gggcaatgac catttcgtcc
    24421 agaacgaccg aattgaacgt ccccagggtg gcggcggagg ttccggcagt ggtcagggcc
    24481 aggccagcca ggatggtgaa ggtcaggatg aatttgtctt tcagatttcg aaagatgagt
    24541 atcttgatct gctctttgaa gatttggcct taccgaatct gaaacaaaac caacaacgcc
    24601 agctgaccga atataaaacg catcgtgcgg gttataccgc taacggcgtt ccggccaata
    24661 tcagcgttgt gcgttccttg cagaactcac tggcgcgacg cacagccatg acggcaggca
    24721 agcggcggga acttcatgca ctggaagaga atttggccat catcagcaac agtgaacctg
    24781 cgcagctgct ggaagaggaa cgtctacgca aagaaattgc agaattacgt gccaaaattg
    24841 aacgcgtccc ttttattgac accttcgatt tacgttacaa gaactacgag aagcggcccg
    24901 atccctccag ccaggcagtg atgttttgcc tgatggacgt ttccggttca atggatcaat
    24961 ccactaaaga tatggctaag cgtttttata ttctgctgta tctgttcctc agcagaacgt
    25021 ataagaacgt ggaagtcgta tacatccgcc atcataccca ggcgaaagaa gtcgatgaac
    25081 atgagttttt ctactcgcag gaaacaggcg gcaccattgt ttccagcgcc ctgaaactga
    25141 tggatgaggt agtgaaagag cgttataacc cggcacagtg gaatatttac gctgcacaag
    25201 catcggacgg cgataactgg gccgatgact ctccgctttg ccatgaaatc ctggcgaaaa
    25261 aattattacc tgttgttcgt tattacagct atatcgaaat tacccgtcgt gcacatcaga
    25321 cattgtggcg agaatatgag catctgcaat ctactttcga caactttgcg atgcagcaca
    25381 tccgcgacca ggatgatatt tatccggtgt tccgtgaact gtttcataaa caaaatgcaa
    25441 cagctaaaga ctaaaactat cagccgggtc attatcgcct ggctgatttt tagctcactg
    25501 taaattatct catttattac atacattagc ttacaatcgc tttaaatatg acagcataac
    25561 ctttacacaa tttagttcca gaaaacaatc attcggaaaa atgattcagt caacacgtat
    25621 ttccctaggg ttatccttta attatttttt atcgttaacg aaaattgatc ctggtcaaaa
    25681 ctatatatct ctgccatcaa taaaatccag cactcacatt gctctccttt ttatggtttc
    25741 tatgggtaca caaaaattaa aagctcaaag cttttttatt ttcagtttat tactgacgtt
    25801 aattttattt tgcattacta ccttatataa cgaaaacaca aatgtaaaac tcatcccaca
    25861 gatgaattac ctgatggttg ttgtggcttt gtttttcctt aacgccgtca tttttctttt
    25921 catgttaatg aaatatttca ctaacaaaca aattttacca acactcattt taagccttgc
    25981 atttttaagt ggccttatct atttagttga aaccattgta attatccata aaccaattaa
    26041 cggcagtaca ctgatccaga caaagtcgaa tgatgtttct attttctata ttttccgcca
    26101 actcagtttt atttgtttaa cctcgctggc actcttttgt tatggaaaag acaacatcct
    26161 tgacaacaat aagaaaaaaa cgggaatcct gttgctggcg ctgattcctt ttttagtttt
    26221 tccccttctg gcacacaatc tgagcagtta taacgctgac tattctttgt atgtcgtcga
    26281 ctactgtccg gacaaccata ctgcgacctg gggaatcaac tatacaaaaa tattggtttg
    26341 tctgtgggca tttttactgt tctttattat catgcgtaca cgattagcca gcgaactatg
    26401 gccgttaata gcattattat gtctggcatc gctatgctgc aacttacttc tactgactct
    26461 ggatgagtat aattacacca tctggtatat cagtcgcggg attgaagttt ccagtaaact
    26521 gtttgttgtg tcttttctga tttataacat ttttcaggaa ctgcaactct ccagcaaact
    26581 ggcagttcat gatgtgctga ccaatattta taatcggcgc tactttttca acagcgtaga
    26641 gtcattattg tcgcgacctg ttgttaagga cttctgtgtc atgctggttg atattaatca
    26701 gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt gataaagtgc tggtttcaat
    26761 tgtcgatatt atccagcaaa gcatccgccc cgatgatatt ttagcgcgac tggagggtga
    26821 ggtgtttggc ttgctattta ccgaactcaa tagtgcccag gcaaaaatca ttgcggaacg
    26881 tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac agatatgatg ttcctgaaca
    26941 aatgaccatc agtattggca cggttttttc aacgggtgac acgcgtaata tctcgcttgt
    27001 catgacggaa gcagataaag ccttacgcga agcgaaaagc gaggggggca acaaagtgat
    27061 tattcatcat atttaagtgc aaaaatattc agagccatgc tttttgcgtg gcttttgcat
    27121 acaatttatt actattaccc ctaaatttcc cctccccagc ggcgcagaga tgagtataat
    27181 tagcgcccct gtgccaggcc gcaatcgaac tttatctggt tttctcgttt cactaaccga
    27241 aggagtgcca tttatcatga aattgcacca tagaatgctc cggcatttta tcgccgcaag
    27301 tgtcattgtg ctgacatctt ccttccttat ttttgaactt gtcgccagcg acagagcaat
    27361 gagtgcctat ctgcgctata tcgtgcagaa agcagactcc tcctttcttt atgataagta
    27421 tcagaatcag agtattgccg cgcatgtgat gcgcgctctc gctgctgagc agtcggaagt
    27481 gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag tctgccaata atacccatgg
    27541 cttaaacctg actgcccata aatacccggg cttacgcggc acactacaaa ccgcatccac
    27601 tgactgcgac acaattgtgg aagctgcagc actattaccc gcttttgatc aggcagtgga
    27661 aggcaaccgc caccaggatg attacggttc aggtcttggg atggccgaag agaaatttca
    27721 ctattatctc gatctcaatg accgctatgt ctatttttat gagccggtta atgttgaata
    27781 ctttgcgatg aataactggt ccttcctgca gtcaggaagt attggcatcg atcgcaaaga
    27841 tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc atttaccagg atcagcgtac
    27901 taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc gcagggcagc taaaagggat
    27961 tgtgctgctg gatattaaca aaaacaattt gcggaatatc ttttacactc atgaccgccc
    28021 tctcctctgg cgttttctca atgtcacgct aaccgatacc gattcggggc gcgacattat
    28081 catcaaccag agcgaagata atctgttcca gtatgtcagt tacgtccatg acttaccggg
    28141 cggcattcgt gtctcgttat ccattgatat tctttacttt atcacgtctt cgtggaaaag
    28201 cgttctgttc tggattttga cggcgttaat tttgctgaat atggtgcgga tgcacttccg
    28261 cttataccaa aatgtgtcgc gagaaaatat tagtgatgcg atgactggac tgtataatcg
    28321 caaaatttta acccctgaac tggagcagcg gttgcagaaa ctggtgcaat ccggttcttc
    28381 ggtgatgttt attgcaattg acatggacaa gttaaagcta ataaatgaca ccctcggtca
    28441 tcaggaaggg gatttagcga ttacgttgtt agctcaggcg attaaacaat cgattcgtaa
    28501 aagtgattat gccatccgac tcggtggcga tgaattctgc atcattcttg tcgattcgac
    28561 gccgcaaatt gcagcacaac tgcctgaacg tatcgaaaaa cgtctgcaac atatcgcgcc
    28621 gcagaaagag atcggcttct cttccggtat ttacgcgatg aaagaaaacg atacgttaca
    28681 tgatgcgtat aaagcttccg atgagcgttt atatgtcaat aagcagaaca aaaacagccg
    28741 ttcatgataa ccttctgtgg ttgtttgctt gtaatctcag gagcgtgaaa tgactgaaat
    28801 ggctaaagga agcgtgacgc atcagcggtt aatcgcttta ttatcacaag aaggtgctaa
    28861 cttccgcgtc gttacccatg aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg
    28921 caccgcttta ggccagggag ctaaggcact ggtatgtaaa gtcaaaggca atggcgtaaa
    28981 tcaacatgtt ctggcgatcc tcgctgccga tcaacaggcc gatctgagcc aacttgccag
    29041 ccatataggc ggattacgcg cttcgttagc cagccctgct gaagtcgatg aactcacagg
    29101 ctgtgtcttc ggcgcgatcc cccctttcag cttccatcca aaactcaaac tggttgccga
    29161 ccctctactc ttcgaacgat ttgatgaaat cgccttcaat gcagggatgc tggataaatc
    29221 cgttattttg aaaaccacgg attatttgcg cattgcgcaa ccagaactgc tcaatttccg
    29281 ccgcactgcg taactagcct gtccgttcaa taagcagaac ggacaaaacc agcacaacag
    29341 caatggcaaa aaacgatgac gtgataatca gtatctcgac gaacatttga tcgttcatag
    29401 catgcccccg ggagatagct gttttcacgt tattgttagc gtgcacaaat ggcagtttga
    29461 tgacagttcg cctgattttt tatgcaaaaa acgtaaagat tttctacttc cttcctgcag
    29521 caagcgtaaa gtaagctggc ttattatttt ttggcaagga aaccacgatg tttgatgtca
    29581 ctttgctgat cctgctcggg ttagctgcgc tgggctttat cagtcataac accactgtcg
    29641 ccgtttcaat tctggtgttg atcattgtcc gcgtcacacc gttaagcacc tttttcccgt
    29701 ggattgaaaa acaggggcta agtatcggga taatcattct gacgattggc gttatggccc
    29761 ccatcgccag cgggacgcta ccgccctcaa cccttattca ctcgtttctg aactggaaat
    29821 cgctggtggc aattgcagta ggcgttattg tctcctggct gggtgggcgc ggcgtgacgt
    29881 taatgggcag ccagccgcaa ctggtcgccg ggctgctggt aggcactgtt ttaggcgtgg
    29941 cgctgtttcg cggcgtaccg gtcggcccgc ttattgccgc gggtctggtt tcgctgattg
    30001 tggggaaaca gtagttaatc ccgcaatata tcggcctggc gtttgcccca ggcctttctt
    30061 aaacatagta ataaaggccg ttgttgaatc ataccccagc gtatgagcaa ctttctgtac
    30121 agtatcgcct ttcactaatc cctgaagtgc cataatcagc tgtaactgct gacgccactg
    30181 gcgaaaactc aaccccgtct cttttactat cagtcgggca agattgcgtt cactcatcgc
    30241 aaaaaagcca gcccattgcc ccaatgaccc ccactcgacg ggccctttcg ccatcatctc
    30301 caccatcgtg cggattttag gatgagaaga aacgggcagg tgcagctgtt gttgtggctg
    30361 ctgagggagt tcatcaaaca ggacctgaat taatctgcgg gtcatcggct cagcacgctg
    30421 agtcgtcgtc ctgtttgcca gggttaaaat caattcccgg cataagggcg atattttcaa
    30481 tgtgcaacat gttgtcggca ttgtcactgc gctgggttcg ataaacagaa aacagagttc
    30541 agcgttagct gtcacttgat tgctatgctt caccccgccc ggtatccaaa ccgcatattg
    30601 tggcggcacc atccacaaag cattttccac cgtacaggta attgcaccat gtagcgccag
    30661 tatcagctgt cctttgcggt gctgatgtac aggactggtt aactcatccg ttccggcgtg
    30721 aatacaaaat gcgaccgcgg cttcatggtg tcggtcgggt tcatagccat tgagattcaa
    30781 cctgtgcatc attttgtccg aatttagcga taatttgtca ttttagcttg attcaacata
    30841 acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg agaagcatat gacctgttca
    30901 acttcattaa gcggcaaaaa caggattgtc cttatcgctg gcattctgat gattgccaca
    30961 acattacgcg tcaccttcac cggcgcagca ccgttactcg atacgattcg ttccgcttac
    31021 tcgctgacga cagcgcaaac cggcttattg accaccctgc cattattggc ctttgcgcta
    31081 atctcacctt tggccgcccc ggtagcgcga cgttttggta tggaacgtag cctgtttgcc
    31141 gcgttacttt tgatctgtgc tggtatcgca attcgctctc tcccttcgcc ttacttatta
    31201 tttggcggta cagcggtcat tggcggtggg attgcattag gcaatgtctt actgccagga
    31261 ttaattaaac gcgatttccc tcattccgtc gccagactta ccggcgcata ttccctgaca
    31321 atgggagctg cggcggcact tggatcggct atggtcgtgc cgctggcttt gaacggtttt
    31381 ggctggcaag gcgcgttgct catgctgatg tgttttcctc tgctggctct ttttttattt
    31441 caccatgact ccagcaccga ccttcgctgg actacgctat agccagatac agcaggttta
    31501 tcaataactt aggcggattc atcatatgcc ggtgctttct gaaacttgac ccggcttcca
    31561 cccgcttggc tcccaacagg ctcctggaaa tcgggtcttc cgaggagtca tcatggcgaa
    31621 aatcaaactc accaagtccg cagtcgatgc ggcacaagcc caagcgcagc cagtcgaact
    31681 gcgcgatacc ctggttcctg gcttcctgtg caagatcacc ccggcaggcc gcaaggtgtt
    31741 catgctccag taccgcacaa acgccggaga gcggcgcaaa cctgccttgg gcctatacgg
    31801 agaactgacc gtcgaacagg ctcgatcgct ggcgcaggag tggctggcgc aagtgcgccg
    31861 gggtggagat cctgccgcag agaaggctgc agcgcgtcag gcacccacag tcaaggaact
    31921 gtgcatcaag ttcatggagg actactccag ccagcgcaac aagccgagca cgcaagaagg
    31981 ctaccagagc gtcattgatc gcaacatcat cccaatgctt ggtcgcatga aggtgcagga
    32041 tctgaagcgg cctgacgtgg cttcaatgat gaagaagatg gcgcacaagc ccgccgatgc
    32101 gaaccgcacg ttcagcgtga tgcgcaagat gttcaacttg gccgaggtat ggggctatcg
    32161 ccctgacggc accaacccat gccgccatgt gcccatgtac ccgaacggca aggcgactca
    32221 cctcatcagc gatgaggaaa tgggcaagtt gtttcgctac cttgaccagt tggaggcgga
    32281 agggctggag catgtagtga tcccgctggc catccgcctg caattcgagt ttgctgctcg
    32341 cctttctgag atcgtctcac tccaatggga ttggattgat ctggaaaacc ggcgggtggt
    32401 gtggcctgac agcaagacag gcggtatgtc caagcccatg agcgaagaag cctatcggct
    32461 tctttcgacg gcggctcgca atgctacgac gccccatgta ttaccgtctc cgcggcaccc
    32521 tgatcggcat ttgaccgacg gcgagtatta caacggttgg actcgcactc tgaaagccgc
    32581 tggcgtgacg catgtgggta cgcacggcat ccgccacagg tctgcgaccg acattgccaa
    32641 ttccggcatc ccggtcaagg tcggcatggt gctgacggcg cataagaccg tggcgatgtt
    32701 catgcgctac gtccacaccg aggacaagcc ggtgcgggaa gcggccgaac tggtggcaag
    32761 ccggcgcaag aggatcaccg gcgccaagca gcgtcccccg gctgcggagg cgacagcatg
    32821 accaggcgca aggcatccat cgcgtcaccc gcagcgccga tggcgctgct gggcgacatt
    32881 cgggcattga ttgaagcgtc gcggcagcgc gccgcctcga cggtcaatgc ggaactgacg
    32941 ctgctgttct ggcagattgg ccagcgcatc catatggagg tgctggccgg gcaacgagcc
    33001 gggtacggcg aggaaatcct gccgaccctg gccgagcagc ttgcgcgcga ctatgggcgg
    33061 ggctttgggg agaagaacct gcgccggatg gtcaaattcg ccgccacgtt tcccgaggaa
    33121 ccaattgtcg cgacactgtc gcgacaattg agctggtcac atttcgtcct gctgctggca
    33181 ctcaaggaac cgctccagcg ggactactac gcgcagatgg caggtaccga acgctggagc
    33241 gtgcggacga tgcgcgagcg catcgactcg atgctgtatg agcgcacggc gctgtcccaa
    33301 aagcccgaag aaaccatcgc gcaggagctg gcgaccctgc gcgacgcgca gcgcatgaca
    33361 ccggcgctgg tcatgcgcga cccgtacatc ctcgacttcc tgggcttgcg ggatagctgg
    33421 caggaaagcg acctggaggc ggccatcatc cgcgaaatgg aagccttcct gctggagctg
    33481 ggcgcgggct tttgcttcgt cgcgcggcag aaacgcattc agattgacga cgaggatttc
    33541 caccttgacc tgctgttcta caaccgcaag ctgcggcggc tggtggcggt ggagttgaag
    33601 atcggcgagt tcaaggcggc ccacaagggg cagatggagc tttacctgcg ctggctggac
    33661 aagcacgagc gcgagccgga agaagcctca ccgctgggga tcatcctttg caccggcaag
    33721 aaacgcgaac agatcgaatt gctggaactg gacaagtcgg gcatccacgt tgccgagtac
    33781 ctgaccagct tgccgccgcg agcggtgctg ggcgagaagt tgcagcaggc aaccgagcgg
    33841 gcccggttgc agatcgagca gcggcagcct ggcgagaagt cctaacagca gcatccgtgc
    33901 gtcccggcgt gccgccgtag ggttcgcggg ctgcgccccg tgcttcgtgc cgaatcacag
    33961 ctatttgcgc tgatccctga cgcctccggc ctggatcgct gatcgagtac cgggagatgt
    34021 tccacggcac catcgacagt gcattggttt atccgcgtga agtggtcaag gaggctctgc
    34081 ggctcaatgc ggcggcggtc atcctctcgc acaaccatcc cagcggaaat cctgagccgt
    34141 cgcaggcgga caaggtgctg acgcagcggc tcaaagaagc gctgatgctg gtggaggttt
    34201 gcatactgga tcacgtcatt gtgggcggta tggaaactgt ctcattcgcc gagaaaggac
    34261 tggcctagcc cggatttctc atgaccgcat aaacaaaaac ggctgaaagc cttatgattc
    34321 ttgtcgccaa acaaagcccc ataagtcttc agccgcgcac aaaatgctac cagaaaaact
    34381 ccacttttca cccctctctc gccgcctcgt cgaggcctct ttcaccggtg gtcacatcac
    34441 ctcggatgcg ggcttgttgc tgctgcgcga ggtcgaccgg caacatggct tgacccgccg
    34501 cctggccaaa atcgttcccg atcggcgtga tcctgggcgt gtccagcatg gactgcagac
    34561 actgctcgcc cagcgtatct atgcactggc cgccggctac gaggatctca acgatcatga
    34621 cggcctgcgc catgactacg cgctgcagac tgcggtcaac cgcctgcaac cgctggccgg
    34681 caaatccact ctggggcgcc tggaacagca agccgatcgc gaaacggtgg tgcaagccca
    34741 tcgcctgctg tgggagcact tcatcgccca gcatgaccag gcgccggctg agatcgtgct
    34801 cgacttcgat gcgaccgatg tgccggtgca tggtgatcag gaagggcgct tcttccatgg
    34861 ctattacgac cattactgct tcctgccgct ctatgtgttc tgtggtcgcc atctgctggt
    34921 gagctatttg cgcccgagca acatcgatgg cgcccgacac agttgggcca tcctggcact
    34981 gctggtcaag ttcattcgcc gcttctggcc agagacccgc atcgtgttcc gcggtgacgg
    35041 cggtttttgc cgacatcgca tgctcgactg gtgtgaccgc aagcaggtgg attatgtggt
    35101 gggtctggcc cgcaacaccc gcctgcagcg gatggcggcg ccggccatgc aagcggtcgc
    35161 cgaggcctac caagaaaccg ggcagaagat gtccggcgtg tatcgcttcc tctatcaggc
    35221 cggtagttgg cgtaggcgcc gcctggtcgt gtcgcgcctg gaacatggcg agcagggtgc
    35281 caatccacgc ttcatcgtcg tgtcccgctt tgccgaggat gctgcccagc agtactacga
    35341 agactactgc ggccgtggcg acatggagaa tcggatcaag gaccaacagt tggatctgtt
    35401 cgccgaccgc acctccagcc cacgctggtg gaccaaccag tggcggctga tgctgtcggc
    35461 ctttgcctat gtgctgttcg aacggctacg cgatcatctc cagggcaccg cattggcgcg
    35521 catgagcatc cataacctgc gcttgaaact gctcaagatc ggtgcggtga tcattcgcaa
    35581 cagccggcgc atccgcgtgc tgatcagcga ggcctatccg catcaggaac tctttgcggg
    35641 gctggttgcc cgcctcaagc cgacctgaac aggcgggcgc ccccggcccg gctcaatggg
    35701 ggaaaggggg cagtgcgccc catacgcggg aattgaataa aaaacgatca atttttagcg
    35761 gtggaaggca ggttgttgat acaccgaggc ttcaccggcc acgcctgccg gcctcatgag
    35821 aaatccgggc tagtcttgga gtttccgccc attttttatc cagcaccaaa catcatatta
    35881 atatgcctgg tatttactct gattagtcac cccgctcagc caaggcacga accaatctaa
    35941 aaagctcggc ctgatatttt gcgtcgtgaa gtgcatcgtg atctcccttt tggttaggat
    36001 gcacaacttt atcaatggca cttgatctag tctcgtacca tgagcaaccc gtagccccca
    36061 taaaaagcgc tttaatatcc agcgcagtga aaccaaatgg attttcgccc agatatttca
    36121 aaaaatagta atttacaaat ccccagtcaa atggggcgtt tagtcccaca aaaaccggag
    36181 agctttcctt ggggacattc ttggcgatcc aagctgaaaa ttccgtcatg gcctcagatg
    36241 gagttacgcc ttcattttca gcttgctcca aagtaaggcc tgtgatcttc agtgcttccg
    36301 caaccgattt ttcactaact ggttttaaca ttatcgaaaa tgagatttcc ggctgataca
    36361 ctaggcaagc acccacggta agcatgctat gctctccgac aatagggcca gcagtttcaa
    36421 cgtcaaccga tataaaaact tcaggtttct tactcatttt ctcgctccgg tattaggttt
    36481 tgactcaacc agccataaca ggagatggct ccaattccgc aacagcctga tgaaagtgtt
    36541 cggccttgag tggagaattc ggaaacgcca gggacacagc cgccggatat aagcgcgtac
    36601 gaatgtcaga ataagtccaa gccggcttgg tttttgttgc agatgtcgct tctaccagct
    36661 tcttgatttc ggttgccgaa aaacccatgt ctcccaaatc gtttgtgaat agctcatttc
    36721 gctcttcatc agtggggcgt gtgaactcct caacaatcga cgcacgtcga atgactgctg
    36781 catcaagagc tgccaatcgg tttgtgcaaa gaatggcgaa gatacgcccg ccatgcttcc
    36841 tcatactgtc tatagattga atcaatgtgt tcacgccgac cttatcttca tggtggctat
    36901 gatcttggct gcgggatgcc cctaaagagt caccctcatc aataattagg aaggctcggc
    36961 cctttcccgg cccgatagat ctggaaatat cttcgaatgc ctgggtgatg agcgttccca
    37021 tctcacccac cttgccggtt ccgcgaactt tgttgctcag cttgaaaaga attgcgtctt
    37081 caattctagc gtcgcgagca agacgatttg caatcgcctc tgcatttgca gttttccctg
    37141 ttcctacatc gccatggaaa atagccaatg gatactggtc attcaagagt tcaatgactg
    37201 cgagcttctt tttatgatgc ttttgactcc agcgctcaag ttcattggat tgcagaatta
    37261 gctggaggcg cttttgtacg cgctggtaac gcccttcaaa acccagtagt ttctgagcct
    37321 gaatatcaag ctgtgtcgtc ggaaggtttg tttttacttc aaatacggat gagttgctca
    37381 tgaaaagtcc ttcctattgg tgccaaagcc gttgctgctg tatccgcgcc ccgcagaaga
    37441 ggttaggtct ttgtgactgg tgtccgtaga cgaaggtacc catgaaatct ttagggggag
    37501 atccgcacgt ttctctgcgg atgatgaatt ggtatagcgt agatgaattc gaatccaccc
    37561 accgttgtct tttggcgtca gaggccatgt tacgccacca ggccgctccg ttcccgttgc
    37621 cccttcgctt tgaaactcat atttgatagc acgcacctcg gaagtgccat tcatgagcgt
    37681 tacgtcggcg tattcgagat aaccgttctt cgccaacagc tctatgtcgt gagcgtagtt
    37741 tgacgcctca tcctcggtca tggccttggt ggtggtcgca ataatgatca agtcggcttt
    37801 gacgctgcgc atgacctttt caacatctgc aactgtataa gtattagtac tcgaataggt
    37861 gctcattatt tttagtcctt ggtcttgaag cgaccaccaa aaatctcttt ccacacttcg
    37921 ttatcatttt cgactgatgc ataggtcgcc gtatccaaag aggattcggc agccttggct
    37981 ataagcgctt gctcgtcttt ggtaatacgg gacgccacat tgttctctgg gcttacaggg
    38041 tcaatgataa ttacaggagt gtcaaagtta ctggtcggtt tgccagcatt ttctggaaag
    38101 tcgatgcgct cccccagctt cgtactggcg atatatgcca agaattctct gaagcgtttc
    38161 tcaatggtca cggcggggcc atctctatca agtagatagc cgaaaatcag ttcgatatgg
    38221 aaagacttga ggcctggcac gtcgtgaaag tgtctccacc gctttgccag gcggactaac
    38281 gtccgaaagt gtttatcctt atcctttcgc gttttaacga actggatgtg gcacggtgca
    38341 caagtgagat tttttgcgcc gctttgaata tcgtattgcc agccgtggtc agcattggag
    38401 ctgtcttgaa gaacggggac aacgtctacc tccaggccgc tcttgacgaa agttaccttt
    38461 gctgcacgac gctgaatctc gaagtcctca actttttttg tggggtaaat ttctatcaaa
    38521 agatcgtaga tcttgttgca aagattttcg tacgactttc cgtcttcatc gactcccgtg
    38581 acataaaaga caacatcaac atccgtagga taatcatcaa cctttcgaag aatagtgtat
    38641 ttcgcaaacg agccggcctt aacgaccttt gtgactttta tctttgattt atcagtaatg
    38701 cgttttttta gctcaacaac aagattgtcc acctgcgcgt gatactcttt gcgcttttcg
    38761 gctggaaggc gcaatacatt gtgatcgaag tattcaagct ctgaatttga taacggcatt
    38821 ggtttccctg cttttattag aaaaattgac ttctctcagt cctgcaatgt catgccgttt
    38881 gcaatgtaat cgacggtgac tgcggctgcc gcttgtgcag caagtagatg aaatctattg
    38941 tcttttttcg agtctccgaa atcactaatg ccacgcacca tcagtactgg tttgccgaaa
    39001 ttggcgcatg cagcaaataa gcccgcccct tccatctcag cgacttcgat ttttccgtga
    39061 atctcgcgca tccctagaaa tttctctgga tcacgaagga gcttttcacc actggcgacc
    39121 gtggccgtct tcggcagaac accttttgcg acagggccag catcaacttg ttcgggcaat
    39181 tcgataccta gagcctggta gcttttcaag aggcgagctt caagacccgt cctgttagat
    39241 aaataggaac taacgtcttg gcgaacacgc attgtcagtc tggtaatttc tgggcgtgcc
    39301 tcactcttgc cgccttcaac gagtgccgcg ccttcatagg cgacgatacg ttctgccaga
    39361 actacctctc ccagagcaca tttttctcgc aggccagccg caatgcccag catcatcacg
    39421 ttgccaggtc gcagatcact caacagcatg gaggttacgg acgctgcatc tacgttgccc
    39481 gcacccgcaa agcaagccaa agcgcagcta gctgtctttc caccgcgctt gacaacaggg
    39541 gctttccaaa cgtgaagacc gttctcagtc gtcgtatgtt cagcatcctc cgcaatacca
    39601 aaagcttgct tggcagcggc caactccacg tccagagctg ttagcactaa tacatcagga
    39661 ggaagggcat caatcctggc tcgctcttcc tcatagaaaa tttttacgat acgtgcgctg
    39721 atttctgaca atgcaagtac gggctcttgc aaagaaatgg caggaagaaa tttgacaacc
    39781 ttattgccac tattcggcat ataggtcagt gggttggtct gaaaattctt aagaacacca
    39841 agtgcaccca aaccgtcctc gctatttgca cccaactgca aatcaagcgg aagaacaggc
    39901 aaaagcttct tggtcatctt gcgtgcgcga tccagtacac cctttccgcc gcctagagcc
    39961 accatcgcag tagcatgctc aatttgctca tcgccgacat tgccgcctgt cagcacgtcc
    40021 tcttctaacg ggacgacttc tgcgacccct cgtgcgacca tgctcatgag cagcttacgt
    40081 tgctcaggat tcgccttgac ttgaagacgt tcgtgcgaag ctacgatttt tagacggata
    40141 tgagagctgt tgccaggcgt taacctgtct accgctttag caaccgtcca gtcgaacagc
    40201 agcgccttcc catccccgtt caccggctcg gctgcgacgt aaatgacgaa gccgccacca
    40261 gcgttaagga cttcctcaac aagggtatcg acaaaagcgt gcgctctgtc tatgagctca
    40321 ttctcggtgg tgttcgagat gcttccagca aggaggatgt ggcttgaagc cagcattagg
    40381 gaatttgtca tgagtgatcc caattgattc tgtgtcttcg cgcataatgt tctgcgcacc
    40441 aattgcttca tccccgttgg cgtagtcgag aagtatgaac caccaagcta atggatattt
    40501 tttcgatata tgcggggtgt tttttacatt tcaagagcgc tgttgcagat ttttattgat
    40561 ctgagggatg tttaacgaag gcttgttttt agccactagt gtcatcggta cgcgtccttc
    40621 aaggatgacg gtgttactcg ttaacttcat catcgggtaa ctccagttca ttccaaagct
    40681 cctcttctgt aagtcgcttg aagtctggca tcaaattttt gactagcgct gggggagcac
    40741 cttccaaggc tgatgtagca atcagagcga atccgccgcg atctaggcga accgcgacga
    40801 ccccatattc ttccaagaca ctacgcatgt tgataacgaa ggcatcccgc aatgtagtgc
    40861 ggcccgagag gattcgtaac gtcttatcgc taacgcgcgc ccgggtcttt cctgttcgct
    40921 tcagtagtac ggccaatcga agtgcaacag tctctataga tggatatttt gtcgtcattt
    40981 gaattccaat acaggattta atcaatccta gattaggatt aaatttttga ttggtcaagg
    41041 ttttccgtct ccaggcaatg cctggccgcg ctgtcgtgct tcgcatcgag ccgcctgagg
    41101 cgtctcgccc ccttcgggct tccatcgttc cctcgctccg ctcggctgac gcctacggcc
    41161 cggcttccag cttcgggcct gcgcgcttcg cttgcgtgcg gtctgcacat cacggcggcc
    41221 gttgccatgt ccagccgtct ctcctgactc catcaccttg ctcgcgactg tagcccgtgt
    41281 ccgcgtgcca tcaaggcgcg tcaggccgtg tcctcggctg cgcctgcggg ccgcacctac
    41341 ctgccgcttt tctccttgac ggcccacgtc cgtgggctcc tgaccgtcgc gggcgatgaa
    41401 ctcaggaaag acggtggcaa cagggccaac cgggttcctc gtgccaaccg caccgaacag
    41461 ccgaaaggct gggctccaaa tctaggaatc cggtgtgcgg tttgaacagc aaaccctttt
    41521 cgtcaggaga aagaccatgc ttgcatcccg tttcgcttcc cgttccccgg tactgcgcag
    41581 cgactccccg ctgtccgatg accagattca ccgcgtggcc ccgtccatct ttgctgaagc
    41641 accccacgaa agccgctccc agcgatacgc ctatatcccc accgccacgg tgctgaccga
    41701 actgcgcaag gaagggtttc agcccttcat ggtgacgcag acccgcaccc gccacgaaga
    41761 ccggcgcgac tacaccaagc acatgatccg gctgcgtcat gccagccaga tcaacgcccg
    41821 aggcgaagcc aacgaaatca tcctgctgaa ctcgcacgat ggcaccagca gctaccagat
    41881 gctggccggg atgttccgtt tcgtgtgcag caatgggctg gtgtgcggcg acaccgtggc
    41941 cgatgtgcgc gtgccccaca aaggcgacgt ggccggacaa gtgatcgaag gcgcctatca
    42001 ggtgctgcac ggcttcgacc gtgccttgga atcacgcgaa tccatgcagg ccatcacgct
    42061 gaacgaaggc gaggccgaag tgtttgcccg cgccgcgctg tcgctcaagt acgacgaccc
    42121 cgacaagccc gcgcccatca ccgagtcgca aatcctgatg ccgcgccgct tcgacgaccg
    42181 ccgcccggac ttgtggagcg tgttcaaccg cacccaagag aacctgacca aaggcggttt
    42241 gcatgggcgc agcgccaacg gacgccgcca gcagacccgc ccggtgcagg gcattgattc
    42301 cgatattcgc ctcaatcgcg ccttgtggct gctggccgat ggtctgcgcc agttgaaagc
    42361 ctgattcccc tgcacggcag gggcaggcag caacccttgc cgcgtcattc gctgctgcat
    42421 tccatcatcg caaggagttt tcaccatgaa cgccatcaac tacaccgaag cccaagccat
    42481 ccacgccacg gccagcaccg cgcctgccgt gctgctggaa gccgccgacc tgagtaagaa
    42541 tctgattctg gtgccgctgt cgcggctggt gtcgcgcccc acgggccgca acgtgcgcaa
    42601 gaccccgcgc atgtcgattc ccgaactcgc cgccagcatc cagcgtgtcg gcctgctgca
    42661 aaacctgatc gtcacctcga ccgctgacgg cgagcgttac gaagtcgtgg ccggaggccg
    42721 tcgccttgcc gccctcaagc tgctggcgaa aaagcaccac atcagcaagg aatgggaggt
    42781 gccttgcctg caggtggccg atggcaccgc ccgcaccgcc agcctgaccg agaacgtgca
    42841 gcgcgaagcc atgcaccccg ccgaccagtt cctggcatta gccgcgctgg tggccgaagg
    42901 ccgccccatc gaggacattg ccgcagattt cagcgtcacg ccgctggtgg tgcagcgccg
    42961 cctgaaactc gccaacgtgt cgccgcgcct gctggccgac taccgggccg aggccgtgag
    43021 ccttgaccag ttgatggccc ttgccatcac cgacgaccac gccgcgcagg aagctgcgtt
    43081 ctacgatgcg cccacatggc agcgcagccc gcacaacctg cgagaccgcc tgaccgagcg
    43141 cgagattgat gcccaccggc atccgctggt gcgcttcgtt gggctggatg cttacgaggc
    43201 cgcaggcggt ggcgcccgcc gcgatctgtt cgcggaagcc gatagcggcg tgtatctgac
    43261 cgatgccacg ctgctggaac gactggcgca ggacaagctg gccagccttg ccgccgaggt
    43321 gaaggccgaa ggctgggcat gggtggatgc cgcccccggc acgacccatg ccgacctgca
    43381 agccttccag cgcgccccga ggcagcgccg catcccgaac aagcgcgaag cccaacgcat
    43441 cgagaagctg caaaccaaga tgcaggcgct ggccgaggcc gtggatgccg ccctggacgc
    43501 cgacgatgaa gaaaaggccg acgctttgca ggaggaaggc gaacacctgg gcgaacagtt
    43561 gcaggcgctg gaagatggct tgcaggacta cggggaagcc gtcaaagctg cagccggtgc
    43621 catcgtcacc atcgaccgca acggcgaggc ggtgattcat cgtggccttc tgcgcgaagc
    43681 cgaggccaag gcactgcgca cgcttgaaca gctgcggcaa ggtttcggca gggagggtga
    43741 agccgggaac gacgacaccg gcgaggaaac cgacgacgcc cccaagcccg ccgccatgtc
    43801 cgaccggctg gcgcagcggt tgagtgccca ccgtaccgcc gcgctgcaaa tcgaagtcgc
    43861 ccggcatccg caggtggcgc tggccgcgct ggtgcatggc atggtgcaga ctgtcttgca
    43921 aggcagccac tacggccacg atctgccgct gggcgtgaag ctgacccagc aagatcggct
    43981 ggaaggcatg gcgccggact ggccggaatc gcccgccgcc gtggcactgc gcgaactgca
    44041 acagttagcg ggtgaagcct tgccggagga cagcaccgaa ctgttcgccg cactgctggc
    44101 gaagtcgcag gatgaactgg tgcgcctgtt ggccgtgtgc gtggcgtcca cggtggacgt
    44161 ggtgacgccc cgcgctacgc cgcaccagcc cggcacagaa ctggcgcagg cggtagggct
    44221 ggacatggcg gcatggtgga agccgaccgc agacggctac tttcagcatg tgccgaaggc
    44281 cgcgattctg gaagccgtgg gcgcgttcgc atcgtcgcac gtcacccggc tggcgaagtt
    44341 gaagaaaggc gacattgcca gcgaagccga acggctggcc gatggcaccg gctggatgcc
    44401 tgccatcttc gccgccgaag ccacgcagca ggccgcgcat gaggtggtga cggacgaggc
    44461 tgccgaagca ccggaggaag tcgccgccgt ggcggatgag cagccgcagg ccgaggcact
    44521 ggccgcttga ccttgcacca cagatagcgc cccggttccg gccgggggcg cttcatgccg
    44581 aggattcccc catgagccac aacaccacga accgcccacg catagccgcg acctacgccc
    44641 ccggcacgat acgcgcccgt cgctggcatg gcgatggcga cgtgcgtggc taccgcccgc
    44701 ctcacggctg gatagcccgc gccgacctga ccgacctaca ccccatcacg ggccgcgcat
    44761 tgccgcgtgc cgtgtggtgg atcatcgaaa ctaaagaata gggagcagcc ccgcgcccaa
    44821 gccgttccgt cttgggcgcg ggaataccaa agctccgtgc gcggcagtgg ccgcacccgg
    44881 tttcaaggct caaagccaaa acgccccttt gccgccttcg gtcaggcggc aaaggggccg
    44941 cgcacaagtc gccttgtgcg caaaagtcat tgaggccatg caagtgcagc ggcgtacgcg
    45001 ccgccgacat tcggatagca cctcgccgca atgggcggcg cttgtgaggc tacgccccgg
    45061 cttgctgcgg caggttgcac gaaagcgtgc cgtgctcgac gctggcggtt cgttgcctgt
    45121 acgtcacgaa ggacggttca ccgacacacc acctggcctc gatgtggagc ttccacgcca
    45181 cgcatcaagc atgtggccgc gagttgttgc aggagctctc ttatcttgtt gctggggcat
    45241 tgatctagcc gcgtcagggc gcgagccggt gaagctgtca tgcgaggatg ccgagtcggt
    45301 catggtctcc attcgggaga ggcggcccgt gcgtccttgg cttgtcgtga atcctggcgg
    45361 tggcgcggct gcgccttggg ctttatgacc gcaaccttgc ggcgaaaaca atttcccctg
    45421 cgctgcgcgc attcctcgcg gggaaaattc ttttcgcctc caggttctcc acgttgttgc
    45481 aaccgctccg cggtgcggcc agcccatccc ccgccgggcg gatcacaaca aggacgcact
    45541 ggcgcgaccc tgttcaaccc gaaaggagaa atcatcatgg ctaacatcgg caccttcacc
    45601 gcagagaaag acggcttcac cggcacgctt cgcaccctga cgttcaacgt caaggtcaaa
    45661 ctggttctca acgacaaggg tgacaacgag agcgcatccg actaccgcct gcaagcggct
    45721 ggccacgaca tcgacgcagc gtggaaaaag accagcgagg ccggacgccc ttacgtgtcc
    45781 gtgacccttg acgatccatc gttccctgca acggtctatg cccgcctaat cgaaggcgag
    45841 gacggcgcac acgacctgat ctggtcgcgc agcaagccca aggccgcctg atagtcgctc
    45901 accgcgcccc gcccattacg gcggggcaca actctatgca cgcatgacct tattccagtc
    45961 gatgctatga ccggcagctt caagctcacg cgcgagtgcc tgtttccagc ccccgccgct
    46021 atccatcgtc tcccaaacga caccggcgaa atcgcttgga atttccaccg ccccgcgttt
    46081 gagagcacat accttgtctc tgcccaggcg accaatgaag taccccaact ccaaaagcac
    46141 attctgacgg gctctgggct caggcgttcc tccttttacg cagccctcat cgtctggcgt
    46201 aagcaaaact actgcgaaac caacatcact atgtgcaacc actttttcga taattgttcg
    46261 tccttggtta gcctgttcgt gcaagatgat ggcttcaagc ccgattcgct caagaaatcg
    46321 agccactgtt tcacgagcac catcatcgtg accatgaaca ataaaaacct tgcgcgaaaa
    46381 ttgagctttt aacatggcat ccgtgcttcc cttggcctgg atatagctct tgtaatcccg
    46441 cacaaatgga atgattagct ggccagtaag tgcgtgaatt ccagcaatga ctttttttcc
    46501 ggaatagaaa aaatggtgtc cgaaattggt agcgtagccg ggatctgccg ccagcttctc
    46561 gattaaaagc aacgtcaacc ccagcgcctg ttttgggtca tctggccaag caagctgggc
    46621 gcttccaacc atgctacccc cggttttttc actttcagcg atgaacgcct caacgtcgag
    46681 accttccgtg agctcggcgt tgtatggctc aaggtcggga tgtcgtagca gttgagccaa
    46741 ttttatgaga ggtctttcgt aggtttgaag ctgtgaggac tgcaagtcta gaactgcatt
    46801 gtttatttcc gagaataagt ctttagccat caatcatccc catccgactc taatctagct
    46861 tctcccgctt tttcaagaat actctcccga tacttttcct tcatttgttc tgcgagaggg
    46921 ccaacgatag ggttcgaaga atgttctcct aagcggtcgt cgatcattcg attgaccgat
    46981 tgaattgcgc cctcgatgct ggcgggatca tttggatcaa agtttacgac cccgagttcg
    47041 ccgtctaatt cactcaaggc gcgctgcgca tctttcaggt ttttctgaag cttatccagt
    47101 ccggtaattt tgaacacgac tcacctcctt ttatttattc ggatgctcgg aaaatgatga
    47161 aacttcccga gcaatgtgat ccatcagttc gtggacgttt tttggatcac tacctgcata
    47221 cgcaaggacc agcagcgtaa gtgggtgaac atccagcact gcacaaacat cttcaatttt
    47281 attcaatgtt gggcttttca gccctcgctc cagagaactg agataagttc ggctggacac
    47341 atccgagaac gcctcctggc tcagacctcg cgcctttctg atccgccgca gtgcatcggg
    47401 caacgatgac ttgaacgctg tcattctgaa ctttccccaa aatccagaat gacaacctgt
    47461 tgcgccctat agggctacaa tctatagtgt tcatttctcc cgtttgtgcc tttactgaaa
    47521 tccgcattgg cggctttctt caaaagcgga gagcctgatc cgtgtctttc cttacttccg
    47581 tgcatgtgtc ttcgtgcctc tacgtctctg cggatacgtg ggattgcgca ttagtgcttt
    47641 cgttggaagg cgtaaaagcg cctctgctgc tccgcaagtc cttggacgta aagccatgac
    47701 tccgctcatc accgccgacg agcgcacgcg cctgctggcc aacggccagg cccgcaccgc
    47761 cgggcaggac accgacccac tgcccgtggt gcgtctgttc acgccagatg cgcatgccac
    47821 ttggctgctg gcttcgctcg accccgccga tggcgatacc gcccacggcc tgatcgactt
    47881 ggggattggc atgccttcac tgggaacggt gaaactgtcc gacctggccg ccatcgtcgg
    47941 gccacgccag cagcccgtga tgcgggatcg ctatttccag ccggtgcggc ggctgtcgga
    48001 atacctgcga ctggccgaag acagcggctc gatcaccgat tgagcgccac cgcgaaacag
    48061 ggcgcactgt gtctatttcg gtctggtctg tgacccgttc ggtctgaatc ggcagtgttg
    48121 caccgaagcg atgcgtgcct gatcgaaacg gtcatgatgc ttggcgcggg ctgcggcaag
    48181 ctgaccgacg cggcatgcca ttggagtgcg ttgccgccgt cgcactcgga cgatttcagc
    48241 aacgcatcgg agttcatcgg tcttgacgct gccagtttac gcttcaccca tgacgttttg
    48301 acgatggcct cgtagcacgc cgtttcgccg tggcgacgca cccccagcac agggaggttg
    48361 cctcatggct gatcgcagtg ccgagccgtg gtatgccacg gcggcctatc tctacgttct
    48421 gcacctggat ggcccggcgc tcgcctggga gtaccttcgc cgccatcccg actaccggcg
    48481 cgactggctg cgccgtcgtc gccaaccgga tgctgccgag cgctgggggc tgcgcctgct
    48541 ggaagatccc gccctggatg cgcgggacgc gcagccggcc tggttccccg atcacgacgc
    48601 cgtggtgcag ctctacccgg atgccgatcc accgcccgat gccctgttct ttgagttctg
    48661 gaaactgccg ggccacaaac acctgatcca tgacggcaag cgtctggtgc tggtggcgcg
    48721 ctggcccggc tgctgcctgc gtttcgtgct cgcgccaggc ttggccgatg gcatggctta
    48781 cgcctatgcc atccgggcct gcgccgcgcc ttgcgaacgc tatgcggccc tggcgagctt
    48841 attgaacaag atcgccgtag ccgccggtgc cgcacctgcg gcgaccgtcc gaccacgtcc
    48901 gccactgtcc gcattgctgg aactgcacac tttgcaggcg ctcgacgcga ccctggcggg
    48961 cgcgtcctta cgcgaagtcg ccgaagggct gtttggtgtg gatgccgttg ccgcagactg
    49021 gcacgccgac agcgctttgc gcgcccgtgt gcggcggctg gtgcgccggg gcgatgggct
    49081 gatgtgcggc ggttatcgcc gcctggcaca gcttccggct gtcatttcgc atgagagggg
    49141 acgtttcgca tccgcagcaa aacgtccctg agcaagagcc tcaagtttct tgagactgcc
    49201 tccatccggc tgcgctgtgt ggccgggctt gatggaggta catcccatgc gacccgctcc
    49261 cttgcggcct gccgccgctc ccgttgccac acccgcacaa ccccagcgct atctcaccaa
    49321 cgacgaagcc gccgactacc tgcgcctgtc accgcgcacg ttggagaaac agcgcgtcat
    49381 cggtggtggc cctaagtttc ggaagttcgg tcgccgcgtt atgtacgccg tggccgacct
    49441 caatgcctgg gcggaaacgc gcagcttcga gaccacttcc gaccccgaat atgccgagca
    49501 ccactcggct gacagccgtg cgcgctgatc ggcgcatcgc cagtggccat cgccatgtcc
    49561 cgcccaccag ggcacgcctt gcagcagcgc gaacagctcg acctgttccg cgcactgccg
    49621 ggcgaggaca tggcaccgcg cgatgcccag gacttgatgg cctatccgtt tttctcgctg
    49681 gcgaagtcgc ggcgcaccgc gccgatcgac ttccgcagtg gcaacatcac catccgcgtg
    49741 gaaggaacac tggagcatgg catcgccacc atctgggatg ccgacatcct gatctgggcg
    49801 gccagccaga tcgtcgaagc caaggacgcg ggcatccagc cgtcccggcg gatgcaggcc
    49861 acgccctacg agatcctgcg cttcatcggg cgcggaaagt cgctgcgcga ctaccagcgc
    49921 ttcaaggccg cgctggatcg gctgcaatcg accacggtgg ccacgtccat ccgcgagacg
    49981 accgggcggc gcctgcaccg cttctcgtgg atcaacgagt ggaaggagct ggccgatgcc
    50041 aacggcaccc cgctgggcat cgaactgatc ctgccggact ggttctatgc cggcgtgctg
    50101 gacgccgccc tggtgctgac catcgacccg acgtacttcc gactcacggg cggcatcgag
    50161 cgctggctgt accgcctggt gcgcaagcat ggcggcaggc aggaatacgg ctggcagttc
    50221 gacttccggc atctgcaccg caagtcgggc agcagtacgc ggttttcgga ttttgcctac
    50281 gacctgcggg cgctggtcgc ccggcaatcg ctgccggggt acgagctggg catcgtgcgg
    50341 attcccgagg acgacatgga attgctgacc ttccggcccg tgccgcagac ggcacgggga
    50401 taacagcggg acaagctgtg gatggggtcg tgctatcagg agtaagaggt gtcgtgctat
    50461 cgggagtacg cctatcgtgc tatcaggagt acgaaaacgc cgaaaagcca gtaacggcgc
    50521 gggtttgcga tctctctaac attactaact taaattctct aactattggt agagaagcag
    50581 cattgcggtg gacaactgcc gcaggccgaa aaaacagcca gcaaaaactc caaaaaccac
    50641 cccgttcggc acggcaaaaa caggccggtc ttccaagctg gagggccacg ccatgatcgt
    50701 cgctctgctc aaccagaaag gcggcgtcgg caagaccacg ctcgctaccc acatcgcggg
    50761 cgaactcgcg ctgcgcggcc agcacgtcgt cttgctcgat gccgacccgc agggttcatc
    50821 gctggactgg acgcagcgca gaagccagca aggcttgcca cggctgttca gcgccgtggg
    50881 cctggcccgc gaaacactgc atcaggaagc gccagaactc gccaaacgcg ccgatcacgt
    50941 catcatcgac gggccaccca ggatcgccgc cctggcgcgc tccgcgctgc tggcggccga
    51001 tcgcgtgctg atcccggtgc agcccagccc ctatgacgtg tgggccagcg ccgagatggt
    51061 ggcactgatt cgtgaggctc agatgttccg gccgcagcta gcagcggcct tcgtcatcaa
    51121 ccggcgcgtc agcaccacgg tgatcgggcg cgaagcgcgg caagccttga ccgaacagcc
    51181 gttgccagcg ctgcgcagcg aggtgcgtca gcgcatcgtc tttgccgaca gcgcggctgc
    51241 gggtcgattg gcgcgggaga cagctcccgg tagcgcggcg gcgcaggaaa tcaccgcgct
    51301 ggtcgatgag ctgctgcggt ggccgtcatg agcgacaaca gcaacccacc caacggcaaa
    51361 cgcccggcca aacgggtcgg cattggcgtg cgtccgcccg ccaatccgca cgccgaggcg
    51421 tggattcgcc agggcgatgc ggatgcgctc aacaagggcg acctctatac cgctcgcctg
    51481 accctcgaca tcacgcccgc catgcgggcg cgcatcaagt tgtcggcctt cacgcaaggc
    51541 gtgaccgtgg ccgacctact gcgcggtttg ctggagcgtg agtttccaga acaccgcagg
    51601 gaaaacacgc catgaccaca tccgcattgt ctgttgccgc accgcccgcc atcgcggtca
    51661 cggctgcgca cacagcgcct tccagccaac ccgccagcgc gccgctgacg cgcgtggctc
    51721 tggcttatat cgaacctcgc ttcaagctct acctgcgctt cggcgaacca gcgcgcacgc
    51781 tccagcttga tcgctggcgg cgctgtgcca tgttcatgcc gggtgcaatt ctctgccgcg
    51841 ttcgttggca ggccaatgac tacggcacga tccgctggca gctcatggtg atgcaggctg
    51901 ctacaccgct tgatgccgtg cagcgcatcc ctggcgtgcg gccgggcgca cgcctgcttc
    51961 tgcacgccga aggcgatgcc agcgtgcgcg ccgtgctgga acgcatcgac ggcatcgagg
    52021 cgctggggat cgctgccatc gacgtgtcgc ccgcgtactg gcgcacgctc gcaaaccggc
    52081 tcgccgcgca ttcgccgctg cccgaataca ccgccgagcg gcacgctgct tggctggccg
    52141 ggagggcgct gccatgacag cccatcccac cgccgcacgc acgcccgaag ccgcaccgct
    52201 gcctcgctcg cgcctgcgcg ctcgcctcgt gctggcgggc ttcgccgcag ccggcctcac
    52261 tgcgctggcc tggacggttt tcgcgcagcc gctgccgcgc ctgatctaca acccatccga
    52321 cagcgttccg gtcggctggt atcgcgtgca gccgctcgac catcgagccg ccgcgctgcc
    52381 acgtcccttg tccgtgggca gcatcgtcct gacccgattg cctgccgacg ctggcgtgct
    52441 cgctgcgcag cgcggctacc tgccggtaca cattccgctg ctcaaacgtg tgggcgcggt
    52501 cgcgccgcaa cacgtctgca tcgtcgccgg gcaggttcgc atcgacggcg tgccggtggc
    52561 cgccgcgctg cctgccgatc ggctgggccg gccgctgcca tcctggcagc attgccgggc
    52621 gctggccgag ggcgagctgt tcctgttgag cgcgaccaac ccggcgtcgt tcgacagccg
    52681 ctatttcggc ccggtcagcg catccgccgt gatcggcgtc gcgcacccgg tctggctgga
    52741 gatacgccca tgatggccgt cgattcgctg cacgttgccg tgcatctcat cgtgccatcg
    52801 ggcatgccgt tcaggctgct atcgccgtgt cgtcgcgcgt gcagcgcggg cgcattcgcg
    52861 ccgcacttcg cgctgtcttc ggcgcagccg tccaacgtgc aggcgtcttg cctcgaacgc
    52921 gctcggcctt cggccattgg cgcgtttgcc ggcgggctgg ctgcgagtgc agcagagccg
    52981 ccgggccgac gatgcccgga gcgacagcga ggggcaaagg cggaaggcaa gacaaaagga
    53041 cgcggcaccg ggccgcgtcg aaagcctgtc tgcacatggg ggtggcgcgg cacggagcgg
    53101 cttcgccgcc gtgccgcgtg gggcgcgagg gtcgcgccaa tgcaggcatg tccgcgtgct
    53161 tcgcacgaca ggacacgcca gagcttgcgg ggagagcagc gatgaccgac cgccgcgacg
    53221 acgatttccg ggtgcgcccg agtgctccga agaaccgggg caagggccag gggcagagct
    53281 ttgtttccaa ggtgctcaag caggcgggca aggccagcgg cggcaagtcg gcggtgcgcc
    53341 gtcctgcggc tggcgggaac ggccagcacg caggccagcg gcccggctcg cgcctggggc
    53401 gcggccatac ggcggcgcgt ttcgcgggcg cgaagctgac gcccatgtcg cggcgtgtga
    53461 ctatcaagac cctgctggtc aaccagcgca acgccagccc gcagtcgctc gccaagcacc
    53521 tgcgctacat cgagcgcgac ggcgccggcc gcgatggcga acctgggcgg gcctacgggt
    53581 cgcagaccga cgaagccgac ctggatgcct tcaaggagcg ggccgccgac gaccggcacc
    53641 atttccgctt catcgtctca ccggaggacg gcgccgagct ggacgacctg cgcacctaca
    53701 cccgtcatct ggtgaaccgc atggaagccg acctgggcac gcgcctggat tgggtggcgg
    53761 tcgatcactg gaacaccgac aacccgcata cccacttgat cgtgcgcggg cgcgacgaca
    53821 ccggcaaaga cctcatcatc gccggcgact acatcgccca cggcttccgc caccgggccg
    53881 ccgagctggc gacggaatgg ctggggccgc gcaccgaact ggagatccag cagaccttgg
    53941 ggcgcgaggt ggaacaggag cggtggacga gtctcgaccg gacgctgcaa cgcgaagccg
    54001 gcgacgatgg cagggtgcag atcgaacagt tcaacgcacc ggcgctgcaa cgccagcgcc
    54061 tgctgctgat cggccggctg caacgcttgc agcgcctggg cctagccgac gagacgcagc
    54121 cgggcacatg ggctgtccat gccgacgcgg agaaaacctt gcgcgccctg ggcgagcgcg
    54181 gcgacatcat ccgcaccatg cagcgcgcca tgagcggcca gccgcgcgag ctggcggtgt
    54241 tcgagccggg ccaagatgac aatgaaagtg gccgaaccat cgtcggccgc gtggccgcca
    54301 aggggctggc cgacgagctg cgcgaccggg gctatctggt catcgacggc gtggacggca
    54361 aggcccacta cgtcgcgctc aatgcccgcg atgagctggc gaactatccc accggagccg
    54421 tggttgaggt gcgcggttcc gccgaagtac gggcggccga caagaacatc gccgcactgg
    54481 caagcgatgg cctgtaccgc accgatcacc acctggcgat cgagcaaggc cgcgtcaagc
    54541 ccggacgcaa cccgcaggag gttgtcgccg cccacgttcg gcggctggaa gccctgcgcc
    54601 gggcgggcat cgtggagcgc gtggccgaag ggctatggaa ggtgccggac gacctggccg
    54661 agcgtggccg ccagtacgac gcgcagcgcc tgggtggcgt ggccgtggaa atgaagtccc
    54721 atcttcccat cgagcggcag gcccgcgtga tcggggccac ttggctcgac cagcaactga
    54781 tcggcggcgg caaagggctg ggcgatctgg gctttggcgg cgacgttaag caagccatgc
    54841 agcagcgcgc caacttccta gccgaacagg gactggcgga gaagcgcggc cagcgcgtga
    54901 tcctggcccg caatctgctg gcgacgctgc gcaatcggga actgacgcaa gccgcgaagg
    54961 acattgccgc cgagaccggc ctggagcatc gcccggtcac ggacggtcag cgtttggccg
    55021 gtatctaccg gcgctcggtc atgctcgcca gcgggcgtta cgccatgctc gacaacggca
    55081 aggggttcag cctcgtgccg tggaagccgg tgatcgaaca gcggctgggg cagcagctcg
    55141 ctgcgacagt acgcggcggc ggggtgtcgt gggagattgg acggcagcgt gggccaacga
    55201 ttggataacg ctatcgactc atcattcacc gtcagcggtt ttgagctgct gcgcatacac
    55261 caccaaccgt aggtgccggt cttcgtcaac cgtcaatgat gtgtgctcaa acggttcggc
    55321 ctgaccgttg atgaccattt tgcgcacgcc gttgcaggac gcatgcacat catgcgtccg
    55381 ccaccacgcc ttgaactccg gcgacacccg ttccagctca tccaccagtt catgaatgtc
    55441 cgcttcctgc gtggcgcggg cgaagtcacg gcggaagctg gagagcatca gcggggcttg
    55501 tgcttcccat tcgtggaaac gggcatgcag ggccggatcg gtgaacagca gccagagcag
    55561 attgcgccgc tcgggcgtgt gcttgccgaa accgaaaagt gcatcagccg gtgcattgaa
    55621 gcccaatacg tcccatcgca gattgagcac gaaggccggg tgcggtaaat cgtgcatcag
    55681 gcggcgcacc atcggcggca gcacgcacca ggtcttgccc ggttcggcgg gcggccgttc
    55741 gtgggccagc aggaacaggt ggcggcgttc ggcagcatcc agttttagca cccgcgccag
    55801 gttatccaaa aaggtggccg acacgccaat gtcgcgcccc tgttccagcc atgtgtacca
    55861 ggtcagacca acaccggcga gcgcggcgac ttcctcgcgc cgcagtcctg gcgtgcggcg
    55921 gcggccaccg ctgggcaagc ccacgtcggc aggcgaaagg cgttcgcggt gcgcccgcag
    55981 gaaagccgcc agatcggagc gggttcgttc cagagttcgc atcatttatc tcgtt
//
LOCUS       DAOFQD010000014        55788 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.014, whole genome shotgun sequence.
ACCESSION   DAOFQD010000014 DAOFQD010000000
VERSION     DAOFQD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.014"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..924
                     /locus_tag="QBF46_000888"
     CDS             <1..924
                     /locus_tag="QBF46_000888"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDQ6673110.1"
                     /translation="VKPKKALNVVLGFILGLFISVGAVLARAMLRRGVEAPEQLEEHG
                     INVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPADSAVEAVRALRTS
                     LHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFIDADLRRGYSHNLF
                     TVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSELLMRDRMRQLLEWA
                     NDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNI
                     KGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(1039..2337)
                     /gene="appA"
                     /locus_tag="QBF46_000889"
     CDS             complement(1039..2337)
                     /gene="appA"
                     /locus_tag="QBF46_000889"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDQ6673111.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(2417..2509)
                     /gene="cbdX"
                     /locus_tag="QBF46_000890"
     CDS             complement(2417..2509)
                     /gene="cbdX"
                     /locus_tag="QBF46_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDQ6673112.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(2522..3658)
                     /gene="appB"
                     /locus_tag="QBF46_000891"
     CDS             complement(2522..3658)
                     /gene="appB"
                     /locus_tag="QBF46_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDQ6673113.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(3670..5214)
                     /gene="appC"
                     /locus_tag="QBF46_000892"
     CDS             complement(3670..5214)
                     /gene="appC"
                     /locus_tag="QBF46_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDQ6673114.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRGLEGDLGYGMLLFRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(5348..6205)
                     /locus_tag="QBF46_000893"
     CDS             complement(5348..6205)
                     /locus_tag="QBF46_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDQ6673115.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(6202..6600)
                     /gene="hyaE"
                     /locus_tag="QBF46_000894"
     CDS             complement(6202..6600)
                     /gene="hyaE"
                     /locus_tag="QBF46_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDQ6673116.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(6597..7184)
                     /locus_tag="QBF46_000895"
     CDS             complement(6597..7184)
                     /locus_tag="QBF46_000895"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDQ6673117.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            complement(7181..7888)
                     /gene="hyaC"
                     /locus_tag="QBF46_000896"
     CDS             complement(7181..7888)
                     /gene="hyaC"
                     /locus_tag="QBF46_000896"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDQ6673118.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(7907..9700)
                     /gene="hyaB"
                     /locus_tag="QBF46_000897"
     CDS             complement(7907..9700)
                     /gene="hyaB"
                     /locus_tag="QBF46_000897"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDQ6673119.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(9697..10815)
                     /gene="hyaA"
                     /locus_tag="QBF46_000898"
     CDS             complement(9697..10815)
                     /gene="hyaA"
                     /locus_tag="QBF46_000898"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDQ6673120.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11242..11329
                     /locus_tag="QBF46_000899"
     tRNA            11242..11329
                     /locus_tag="QBF46_000899"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11276..11278,aa:Ser,seq:tga)
     gene            11536..12195
                     /gene="yccA"
                     /locus_tag="QBF46_000900"
     CDS             11536..12195
                     /gene="yccA"
                     /locus_tag="QBF46_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDQ6673121.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            12286..12615
                     /gene="tusE"
                     /locus_tag="QBF46_000901"
     CDS             12286..12615
                     /gene="tusE"
                     /locus_tag="QBF46_000901"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDQ6673122.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(12612..12890)
                     /gene="yccX"
                     /locus_tag="QBF46_000902"
     CDS             complement(12612..12890)
                     /gene="yccX"
                     /locus_tag="QBF46_000902"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDQ6673123.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            12985..14175
                     /gene="rlmI"
                     /locus_tag="QBF46_000903"
     CDS             12985..14175
                     /gene="rlmI"
                     /locus_tag="QBF46_000903"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDQ6673124.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            14233..14550
                     /gene="hspQ"
                     /locus_tag="QBF46_000904"
     CDS             14233..14550
                     /gene="hspQ"
                     /locus_tag="QBF46_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDQ6673125.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(14595..15008)
                     /locus_tag="QBF46_000905"
     CDS             complement(14595..15008)
                     /locus_tag="QBF46_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDQ6673126.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            15181..15843
                     /locus_tag="QBF46_000906"
     CDS             15181..15843
                     /locus_tag="QBF46_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDQ6673127.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            15939..16397
                     /gene="mgsA"
                     /locus_tag="QBF46_000907"
     CDS             15939..16397
                     /gene="mgsA"
                     /locus_tag="QBF46_000907"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDQ6673128.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(16429..18483)
                     /gene="helD"
                     /locus_tag="QBF46_000908"
     CDS             complement(16429..18483)
                     /gene="helD"
                     /locus_tag="QBF46_000908"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDQ6673129.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            18606..19052
                     /locus_tag="QBF46_000909"
     CDS             18606..19052
                     /locus_tag="QBF46_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDQ6673130.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            19062..21224
                     /gene="yccS"
                     /locus_tag="QBF46_000910"
     CDS             19062..21224
                     /gene="yccS"
                     /locus_tag="QBF46_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDQ6673131.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(21187..21816)
                     /gene="sxy"
                     /locus_tag="QBF46_000911"
     CDS             complement(21187..21816)
                     /gene="sxy"
                     /locus_tag="QBF46_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDQ6673132.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            22035..22544
                     /gene="sulA"
                     /locus_tag="QBF46_000912"
     CDS             22035..22544
                     /gene="sulA"
                     /locus_tag="QBF46_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDQ6673133.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22901..23953
                     /gene="ompA"
                     /locus_tag="QBF46_000913"
     CDS             22901..23953
                     /gene="ompA"
                     /locus_tag="QBF46_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDQ6673134.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(24028..24480)
                     /gene="matP"
                     /locus_tag="QBF46_000914"
     CDS             complement(24028..24480)
                     /gene="matP"
                     /locus_tag="QBF46_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDQ6673135.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKHDLQALLGKE"
     gene            24666..26426
                     /locus_tag="QBF46_000915"
     CDS             24666..26426
                     /locus_tag="QBF46_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDQ6673136.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDVLPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            26495..27013
                     /gene="fabA"
                     /locus_tag="QBF46_000916"
     CDS             26495..27013
                     /gene="fabA"
                     /locus_tag="QBF46_000916"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDQ6673137.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(27083..27250)
                     /gene="rmf"
                     /locus_tag="QBF46_000917"
     CDS             complement(27083..27250)
                     /gene="rmf"
                     /locus_tag="QBF46_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDQ6673138.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(27506..28069)
                     /gene="pqiC"
                     /locus_tag="QBF46_000918"
     CDS             complement(27506..28069)
                     /gene="pqiC"
                     /locus_tag="QBF46_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDQ6673139.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQGGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            complement(28066..29706)
                     /gene="pqiB"
                     /locus_tag="QBF46_000919"
     CDS             complement(28066..29706)
                     /gene="pqiB"
                     /locus_tag="QBF46_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDQ6673140.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(29711..30964)
                     /gene="pqiA"
                     /locus_tag="QBF46_000920"
     CDS             complement(29711..30964)
                     /gene="pqiA"
                     /locus_tag="QBF46_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDQ6673141.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(31094..33001)
                     /locus_tag="QBF46_000921"
     CDS             complement(31094..33001)
                     /locus_tag="QBF46_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6673142.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAVPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(33013..35121)
                     /gene="rlmKL"
                     /locus_tag="QBF46_000922"
     CDS             complement(33013..35121)
                     /gene="rlmKL"
                     /locus_tag="QBF46_000922"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDQ6673143.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            35365..36474
                     /gene="ycbX"
                     /locus_tag="QBF46_000923"
     CDS             35365..36474
                     /gene="ycbX"
                     /locus_tag="QBF46_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDQ6673144.1"
                     /translation="MATLIRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(36471..37013)
                     /gene="zapC"
                     /locus_tag="QBF46_000924"
     CDS             complement(36471..37013)
                     /gene="zapC"
                     /locus_tag="QBF46_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDQ6673145.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(37187..38197)
                     /gene="pyrD"
                     /locus_tag="QBF46_000925"
     CDS             complement(37187..38197)
                     /gene="pyrD"
                     /locus_tag="QBF46_000925"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDQ6673146.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(38308..39045)
                     /locus_tag="QBF46_000926"
     CDS             complement(38308..39045)
                     /locus_tag="QBF46_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDQ6673147.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSIFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(39011..39526)
                     /locus_tag="QBF46_000927"
     CDS             complement(39011..39526)
                     /locus_tag="QBF46_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6673148.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(39534..40076)
                     /locus_tag="QBF46_000928"
     CDS             complement(39534..40076)
                     /locus_tag="QBF46_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6673149.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(40088..41158)
                     /locus_tag="QBF46_000929"
     CDS             complement(40088..41158)
                     /locus_tag="QBF46_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6673150.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEANTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLYECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(41149..43749)
                     /locus_tag="QBF46_000930"
     CDS             complement(41149..43749)
                     /locus_tag="QBF46_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDQ6673151.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQANSGIIDDDGSLYMAGLPAKGTISVRWGEAPDQICHINYELTEQQINS
                     AITRMDAICR"
     gene            complement(43774..44475)
                     /locus_tag="QBF46_000931"
     CDS             complement(43774..44475)
                     /locus_tag="QBF46_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDQ6673152.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKRKEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(44558..45097)
                     /locus_tag="QBF46_000932"
     CDS             complement(44558..45097)
                     /locus_tag="QBF46_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6673153.1"
                     /translation="MKKSVLTAFITVVCVTSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            45453..46028
                     /gene="ssuE"
                     /locus_tag="QBF46_000933"
     CDS             45453..46028
                     /gene="ssuE"
                     /locus_tag="QBF46_000933"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDQ6673154.1"
                     /translation="MRVIILAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            46021..46980
                     /gene="ssuA"
                     /locus_tag="QBF46_000934"
     CDS             46021..46980
                     /gene="ssuA"
                     /locus_tag="QBF46_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDQ6673155.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPESKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            46977..48122
                     /gene="ssuD"
                     /locus_tag="QBF46_000935"
     CDS             46977..48122
                     /gene="ssuD"
                     /locus_tag="QBF46_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDQ6673156.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGESVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQQLNPQGEAVANDFIPRKVAQS"
     gene            48134..48925
                     /gene="ssuC"
                     /locus_tag="QBF46_000936"
     CDS             48134..48925
                     /gene="ssuC"
                     /locus_tag="QBF46_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDQ6673157.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            48922..49689
                     /gene="ssuB"
                     /locus_tag="QBF46_000937"
     CDS             48922..49689
                     /gene="ssuB"
                     /locus_tag="QBF46_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDQ6673158.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(49732..52344)
                     /gene="pepN"
                     /locus_tag="QBF46_000938"
     CDS             complement(49732..52344)
                     /gene="pepN"
                     /locus_tag="QBF46_000938"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDQ6673159.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            52610..53812
                     /gene="pncB"
                     /locus_tag="QBF46_000939"
     CDS             52610..53812
                     /gene="pncB"
                     /locus_tag="QBF46_000939"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDQ6673160.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            53981..55381
                     /gene="asnS"
                     /locus_tag="QBF46_000940"
     CDS             53981..55381
                     /gene="asnS"
                     /locus_tag="QBF46_000940"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDQ6673161.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT        13309 a        14667 c        13859 g        13953 t
ORIGIN      
        1 gtgaaaccga aaaaagcgtt gaacgtggtg cttggtttta ttcttggcct gtttatttcg
       61 gtgggtgccg tgctggcgcg agcgatgttg cgtcgtggtg tagaagcacc ggaacaactg
      121 gaagagcacg gcatcaacgt ttatgccact atcccaatgt ccgagtggct ggataaacgc
      181 acccgtctgc gtaagaaaaa tttattttct aatcagcagc gccatcgtac taaaaatatc
      241 cccttcctgg cggtggataa cccggcggat tctgctgtgg aagccgtacg tgcgctacga
      301 accagtctgc acttcgctat gatggagacg gagaataaca ttctgatgat caccggtgcg
      361 acgccagaca gtggtaaaac gtttgtcagt tcaactctgg cagcggtgat cgcccagtcc
      421 gatcaaaaag tgttatttat tgatgccgac ttacgccgtg gttattcgca taacctgttt
      481 accgtgagta atgaacatgg cttgtcggaa tatctggcag gtaaagatga gctcaacaaa
      541 gtgatccagc attttggcaa aggaggcttt gatgtgatta ctcgcggtca ggtgccacct
      601 aacccgtctg aactgctgat gcgcgatcgg atgcgtcaat tactggaatg ggcgaacgac
      661 cattacgatc tggtgattgt cgatacgccg ccgatgctgg cggtgagcga tgccgcggtc
      721 gtggggcgtt ctgttggcac cagcctgctg gttgcgcgtt ttggcttgaa caccgccaaa
      781 gaggtgagtt tgtcaatgca gcgtctggaa caggcaggcg tcaatattaa aggcgctatc
      841 ctcaatggtg tgattaaacg cgccagcacc gcttacagtt acggctataa ctattacggt
      901 tatagttact ccgagaaaga gtaaaactga aagtggccgg atgcggcgtg aacgcctttt
      961 ccggtctacg cattagacag ttcttcgttt gtcatcagcc tcagagcatt caggtaactg
     1021 aatgctcttt tttatgcatt acaaactgca cgccggtatg cgtgcttcat tcacgatttg
     1081 cgtaaaacct gccaacgaac acatgccctg cgcatttcgc tcttcacatc ctgccagggt
     1141 cagtttcacc tctccgggcg gcgtatttaa tgacagcggc gttttatcac gcatctgctg
     1201 taaagtctgg aagaccagcg aaacctgaat ccactggctg ttatcgctta gccgacgcca
     1261 gcgttcaaac accagttcac cacctggcgg cgtgttatcc ggctgaccgg gaagcgtcca
     1321 gttgagctcc agtgcgccgc cgagatttgc cagattagta tcgtgtccgg cgataaacag
     1381 cactgaagtg ggtaatgtca caccatacgc ctgtttttgc ggtggatggg gcgtcaacgc
     1441 tgtcttgatc aaatctaata acggggtggc gcggctgcgg gcaacctctg gcgtgcgttg
     1501 tagcaaataa aattgcgcgt tatgcaaact tagcaaggtg ttccactggt gtgaatcggt
     1561 gatccttccc caccccggct ccggcattcc ctgtgcttgt tgcaggagaa atatctccgt
     1621 cagcattgat gcgaggctta ccgcaccggt taatgagaca ttgtcggcgc tcaccttgag
     1681 ttccgatggt aatgcctgcg ttaatgaaca gctttcgtcc tgtttctcac gtttaaggca
     1741 caagtttgat tgcggaaaat taagcacccg ttccagttcg cgaaacgccg tttgccgatg
     1801 cccggtaaag tcagcaattg accctcctgc cctgctgagg atcgcgtcag tcacgttcgc
     1861 gttatccagt tggcaaacgc cagtttttag aggattaaat aacggatcgg gactggacgt
     1921 atctgcctgg gtatgtacgg ttattgcaca gtcaggtgcc agcccggcgg cgaaggcttc
     1981 gcctgtttta cgggtacgct cgtcgacatc agcaataatc gcgacctgac cagactgcgg
     2041 gcagcccttt ttcgccagca atccgtcggc taccagacgc tggcgttggt aatgtccgag
     2101 ataggcgatt agctcaccac cgcgcggtgt cagccaaccc agttttaccg gccaggttgg
     2161 ccatgcgtct ggggtgacat cctgcatcag ttgcgtggcc ttggttggag cacgcacacc
     2221 atgacgactg acaatcacca cactttccag cttcagctcc ggctcactct gagcgaatgc
     2281 agattgcggg gttaacggaa tcagaagaga taaaaatggg attaagatcg ctttcatcga
     2341 tatgtttccg cttttcatat ctgacattat tgattgcctg atgcgatgct aatgcgccgc
     2401 atcaggccta cgttcgttaa cttttcagac gcgggtccag ccataccaac acaagggtgg
     2461 agagcgaaca catcaacaaa atgccgacga accaaagtaa ataccacatt gttcctgctc
     2521 cttagtacaa ctcgttttcg ttacggcgga gagtttctgt tgtcatgcgt ccccacattt
     2581 tgtagtagct ccagagagtg tagagcaaca caatgggcaa aaatatcagc acgattacca
     2641 acataatgct cagcgtcagc tgactggaag tgctgtccca caacgtcagg ctggagattg
     2701 gactcacgct tgacggcatg acaaagggga acagcgtgat gcctgccgtg aaaatcacgc
     2761 caaattgcat caatgacgcc attaaaaatc cccaacccgg gcgaccacga taaatcgcca
     2821 tcaccgtcag caatgggcag aagaacccca gcagcgggaa gatccacaaa acgggcgatt
     2881 cgacgaaatt attcatccag gcaccaggta gcactgccac cagtttcatt aacggattgg
     2941 aaggaccgtt agcatcctgg gcgagcagta caaagccatc aataccgacc cacagccagt
     3001 aacccgccag caaaaagcac agcatcacca acagtgcagc gcgtttggtg gccagctgtg
     3061 aacgcagatg aatcacacca acagttttca gttgtaacca gacgccacct tgcaaaatca
     3121 ccatcccaag gctgagcaat ccgcacaata aagggaatgg cgtcagcagt tgccagaagc
     3181 tgccgagata ctccacgcgt aattgcggtg tgaaggcaaa cggcacgccg agcaacaagt
     3241 tgccgaaggc gataccgaag actaccggcg gcaccagact gccgatgacc agaccggcgt
     3301 cccacatttt acgccagcgt gcatcggcga tttttccgcg ataatcaaag gccagcgggc
     3361 ggaagaacag tgagcacagc accaggatca tcgccacata aaagccggaa aacgccgctg
     3421 catacactct gggccaggcg gcaaataatg ccccaccagc gaggatcaac cagacctggt
     3481 tgccttccca gtgtgcacca acgctgttta tcactatccg gcgttcatca tcattacgcg
     3541 ccaccagcgg cagcagacag ccgatcccca tgtcaaatcc gtcggagatc ataaagacca
     3601 ccaggatcac gccaatcagc agccaccaga tgaagcgcaa tgtttcataa tcaaacatga
     3661 ttttctcctt tacccctgtt gctgcgtcgg ttgttcactc tgcatcgcgc tcggccccag
     3721 acgggcatat ttctgcatca ggtagacttc ggcgattaag aacagggtgt aaagccctac
     3781 gatcatgatc agtgagaaag ccagttgtcc tgtggttaaa gcggagtgcg cggagtatgt
     3841 cggtaagatg tcctgtatcg cccacggctg acgaccaaac tcggtcataa accacccggc
     3901 ttcaatcgca atccacggca acggcaaact ccagagcgcc attttcagca cccagcgatg
     3961 ctggtcgatt ttgccacgca gcgtctggac aagcgcaatc agcatcacca gtagcagcag
     4021 ggaaccacag cccaccatga tgcggaaact ccagaatacc ggcgcaacct gaggtatcgc
     4081 gccacgcatc gccgcctggt actgtgcggc tgtgacatga ttcatatccg gcgcatagcg
     4141 gaagagcaac atgccgtagc ccaggtcacc ttccagtccc cggaatgcct gcaacacatg
     4201 cggctcacga ttgccttgcg atatttcctg cattaacagc caggccatac gtccgcgttg
     4261 caagcgtggg taggtttcag ccatcaaatt cttcagaccc ggcacgggtt tatctaatga
     4321 gtgagtggcg aggatcccta gcagcgcggg aattttgagg gcaaaggcgt tacgctcttg
     4381 atcctgttcc ggccaggcaa ccacatggaa cggtgcaggt gcaggttccg tttgccactc
     4441 cccttccatc gccgccagtt ttaccggttg tacttgcgcg acttcatacg cagaactgtc
     4501 tccgagttgc agggtaccga taatcgccag agtaccgaag acggaaccga tggcaaacga
     4561 gcgtaatgcg acatcgcgct cccgtccgcg cagtaaatac caggcgctga tcgccataat
     4621 aaacatggcc ccggtcacgt agcccgccat tacggtgtgc acaaatttca cctggctgac
     4681 cggattaaag accagctcgc tgaagctggt catctccata cgcagggtgt cgatatcaaa
     4741 atgcgcaccg gtcgggtatt gcatccaacc gttggcattc aatatccaca acgcagagag
     4801 atttgaaccg aacgccacca gccacgtcac cagcaggtgc tggtatttat tcagccgttg
     4861 ccagccgaag aagaacagcc cgacaaaggt ggattcgagg aagaaggcca ttaatgcttc
     4921 catcgccagc ggtgcgccaa aaatatcgcc cacatagttg gaatagaatg accagttagt
     4981 accaaactga aactccatgg tcaggccggt agccacgcca agagcaaaat tgataccgaa
     5041 gagcttaccc cagaagcgcg tcatatcgcg gtagattgtt ttgccggtga ccacgtaaat
     5101 ggtttccatg atagccagca aaaaaatcag ccccagggta aggggtacaa ataaaaagtg
     5161 atacagcgcg gtcagagcaa actgccagcg cgataaatca atgacatccc acatgcgcac
     5221 tcctgtaggc gaagagagaa aaataacccg ataatggtag atctccctct ttatcctgaa
     5281 actctacgac cagaggtatt cgccgattgc gatattcata agactgaaag cgatacttac
     5341 cgtcttttta cgtcggtgca gcttccgcca gccactgaca tacctcgcta agccgctgcg
     5401 cagagtcgac caaatcttct ggcgctgcca gcaccacttc cggtattggg cagatttcat
     5461 aactttccag taacgggcct tttaaggtgt ccgtacagcg taaatgccag acatggcgta
     5521 accccgtggc gttgatatag ctctcgccat agccaatggt acgaatctga atatttcccg
     5581 gcccacagag acgtgagaga aaaagccgat cagcctcgct gatgggtaat tgcgtcagat
     5641 taatgctgtg cggctgcgcg tcggggttac gtacatgtgc cagtaactca tgcgccaacg
     5701 gtaggccatt catcaggcca tcaatgggcg gcggtaacag cgaatctgtc ggcaagagat
     5761 tttgcgttgc cgcctgccac aacgccagcg gcgcgcagcc agcctccagt ttgtcctcca
     5821 gcaacttttc gccgcgacgt ctgcgcaccc gccataatcc gcagaagatc gcctcctgta
     5881 tttcactttc actgtcgtca gcctgctgaa tgcgcactga cacttcccct tccccgagaa
     5941 gcgtattgat aaagtgacga tcgtcggcgt tgagatttaa cagatcgact gccagcacct
     6001 cgcccaacgg cggttggcgt tcgcttagtt gatgttgtaa ctcgtttaac agcgcaatca
     6061 cctgcgggct gtgagcacat tgctccaggg ccgccatact cggttcatca ttcacctgac
     6121 aggtgatcgg cagtggattc atgctgaaac tgtcatcgtt cggttgcgtt cctggcccca
     6181 gcagatggaa aaaagtttcg ctcatgaggc acgctcctgc tgcggttcga caagcccgcg
     6241 catcaggttt atcagttccg cccacgggtg aataccattc agcacgccgc gatagtttcc
     6301 gccggtaaac actaaagtgg caggaaagcg aaagacgcca aagcgatcgc cgatggcttc
     6361 gctctgctca aggtcagcaa tcgccacctg ccatgtatag tcgggaaact cgcgcagtaa
     6421 ttcgccaatc attaccggat tatcgctgac ctctggcgtg cgtttcgggt cactgcttaa
     6481 taacaccacg ccgtctggcg cttgcgtaag ccagtcgtca agacgggatt cactgactgg
     6541 cgtccagccg cgcgccagca ttcgttgcca caacgcatca aatggcgtgt cgttgctcat
     6601 ccctgctcct cctgtgtcat ccggtactgg cgcaagcgaa cgccttcgta attttccatc
     6661 gacagacagt cataattgag acagcgcgat tcattagccg gttgcggcac aattccccac
     6721 gcagcaagct gcgccagcgc cgcctgttcc gcagcgggca gttgctcccg tgccagttcg
     6781 ctcaggctac cgccgtagtc gtcgagcatt gcgggttgca gaccgacgag ggcaatatgt
     6841 gctggcagat gtccgcggat atccgccagc gccaacactt cggagaaact gttctgatgc
     6901 aggctcattt tcttcgcgct gagataagcc ggaatgcgtt ctccggcata ggttcgcagc
     6961 gttccaggtt ccagcccgta gtcaatggca tcgagaatca acagatggct ggcgctttcg
     7021 acatacccca gcaagttcag tccctgagta ccgccatcga caatctccac atactcgggc
     7081 cagtggtaat gggcatacag ccgttccgcc acccgcacgc cgaagccttc atcggcccac
     7141 agcaggttgc ccagccccat gaccaccacg cgttgctcgc tcatgaacgc tccttgttac
     7201 ttattttgcc aaatttgtgg ctacggtagc cgttgaccat ggtggagatc accgtgtcgt
     7261 cggacatgat gtcttcacgc agcgccatgt agacatgacc gatcacaaac gcgccaatca
     7321 gccacatccc cagccgatgc cagctgtgaa tgtccattga gttgccaccc gtccagtaga
     7381 aaaattccac cacataacgg aacggcgcaa aaatagcgta ctggctgtgt tcgctgtaca
     7441 gcgcaaaacc agtgatgatc ataaagaccg acatcaggaa atagccgaac atcgccgcct
     7501 gggcgatggg attatggcct atatcggcac tcggacgttt tgccagaaac agataccagc
     7561 ggatttcata ccacacgccc tgccaccagc ttttacgcca taccggcacg ataaacagct
     7621 cgcgggagta tcgattgcca acaaaagccc agtagatccg catcagcaaa accacggtaa
     7681 aaaccatccc ggcgctgaag tgaattaacc tgatgtagcc catatagaac agatacgtcg
     7741 cctcgccgct gacggaaggt agcggcttgc cgataaagta tccggtgacc atcaacaccg
     7801 ccatgcataa caccgtcaac cagtgccaga tgcggactgg cgcttcaaag acatagtggc
     7861 tgacaacgtt gtcgcttttc tgttgcatga tgattctcct tcgctgttaa cgcacctgca
     7921 cggagatcag ctcgctaccg tcgtcgccca gcacgtgtgt tgaacaggcg aggcacgggt
     7981 caaagctgtg cagagtacgc aggatctcca gcggttgctc ggggatcgcc attttggtgt
     8041 tcatcagcgc cgcttcataa gcgccaatct gccctttggg atcgcgcggg ctggcgttcc
     8101 aggtggtcgg caccacgcac tggtagagat caattttgcc atcgcgaatg gcggcccagt
     8161 ggcctaacgc cccgcgcggc gcttcggtaa aaccgacacc acggcactct gtcggccagg
     8221 ttgcaggttc ccatttttcc gtggaagcag tggcgagatt gccgtttttc aggttggtca
     8281 tcagcttgtc gaagaaatac tgcaacttac ctgcggccca ctgcgcttcg tgcgcgcggc
     8341 acaaaatgcg gcctaacgtt gactggatac cggaaagcgg caggttcaac gccgacatca
     8401 tgcgatcgac cgactcaacg gtcgcagcat cgcctttgtg ataagcgatt aacgtgcgcg
     8461 ccagcggccc cacttccatc gcgttaccgc gccagcgtgg cgctttgatc cacgagtagc
     8521 gttcctgttc attcagctgc tgaatgttgg tatcgctgcc tttgacatcg ccggggttgt
     8581 accacgggtc ggtgatgcca tcgaacggat gacgcccgac ctgatcgttg ggatatcgat
     8641 accaggcgtg gtcgacaaac tcctgcacct gctgcggatc aaccaaatcc actggcagca
     8701 cattgttgaa gtcgccgtta atcaccgcgc cgccaggcat cagcagactt ttctcgccaa
     8761 agtcgttggc aatatccggg aatgcgccgt agctgagaac gcatttatca gaaagaccag
     8821 tgccgatttc gctccacggt ttgttgaact gaccgatggc taaggcgtcg gggatcatca
     8881 cgttgttaat gaagtccgcc gtgcgggtga taattgactg caccaggttc aggcgttcca
     8941 tattgactgc cccgaccgcg ccgctttcgt caatgttgat ggcgcaaggc atcccgccga
     9001 caatccagtt tggatgcggg tttttaccgc caaagaccgc gtggattttg acaatttcac
     9061 gctggaaatc gagagcttcg agatagtggg caaagcccat caggttagct tctggcggca
     9121 gtttgtactg cgggtgcccc cagtagccat tgcggaagat ccccaactgc ccgccttcaa
     9181 caaatttttt caggcggttt tgtacgtcga agaaatagcc aggggatgat ttcggccaag
     9241 aggagagact ttgcgccagt tcggaggttt tccgcgggtc ggctttcagc gcatctaaca
     9301 catcgatcca gtccatcccg gcaagctgat agaagtgcac cagatgatcg tggcaccaga
     9361 gcgttgccag cataatgttg cggatgatat tggcgttgtc cggcacttta ataccgatag
     9421 catcttcgat ggcgtaaacc gaagccaggg cgtgtacgcc agtacagacg ccgcagatac
     9481 gttcaacgaa cgcccacgca tcgcgcgggt cgcgcccttg taggatgatc tccagcccgc
     9541 gaaacatggt gccgcaggag acggcattgg tgatcacatt ctgatcgtta atattcactt
     9601 cgcagcgcat gtggccttcg atgcgcgtaa tcgggtcgac caccaggcgg cgtccggcat
     9661 tattgatggt gtatccctga gtttcgtact gagtgctcat gcctgtttat cctcattgcc
     9721 tggctgatgt tcggtttctg taggttgctg gttatgacgt ctgcgctggt caacggcgct
     9781 ggcgactgcg tgcacaccaa cagccgctgc caccacgcca agcgcggtta aaccgacggt
     9841 atcggcggtg gaatgagtac ccatttgcgg aatatcgacc acgcggctgt agaacgaacc
     9901 gcgatcccag aaaccatttt ccgcacagcc caggcagccg tgaccagact ggattgggaa
     9961 agaaacgcca tcattccagc gtgtggagga acaggcgtta taggtggtag gccctttgca
    10021 gcccattttg tacaggcagt aacctttgcg ggcagcgtca tcatcccaac tctggacgaa
    10081 ctctccggcg tcgaagtggg cgcggcgata gcatttatcg tggattcgct gaccatagaa
    10141 catcagcgga cggcccattc tgtcgacatc tggcaagcga tcaaaggtca ccatgtaagt
    10201 aatgatggcg ctcatcacat ccgggatcgg cgggcagcca ggtactttga taatgggttt
    10261 gtcggtgatg actttgtcga taggcgttgc ctgcgtcgga ttgggtcgcg cggcctgcac
    10321 gcagccccag gacgcgcagg ttccccaggc gataatcgcg ctggctccgg cagcggcacg
    10381 tttgagtttc tcaataaacg gtcgaccgct gctgatacag aacatcccct gctcgcccag
    10441 cggcggatta ccttctactg cgaggatata tttgccattg tattgcgtga tgatgtcttc
    10501 aaagacttct tccgcctggg ttccggcggc agccatcaaa gtatcgtcgt aatcgaggga
    10561 aatcagggaa aggatgacgt ccttcgccag tgggtgagcg gagcggataa aagattcggt
    10621 acagcaggtg cattccagac cgtggatcca taccaccgga atgcgcggtt tgttctccag
    10681 cgcccaggca atctttggtg ccattcccgc gcctaatccc agcgacgtgg cagccagact
    10741 acaatatttg agaaagctgc gccgggtaac gccctgacgc cgcatggcct ggtaaaatgt
    10801 ttcctcgtta ttcatatcgc acgtctctcc tccttgcgac accggcagga caccgggcgc
    10861 ttatggtcgg caagcgacgt ccctgtagca aggcaaacca tcgcttcacg tgagatgctg
    10921 aaaacgaaag ctcatccttc tgcactggcg cacgtcgcca gaaagtattg ttaataaagc
    10981 gtagtgaaac ttttgcacaa aacaatacaa actgtgtgga tttatctttt agcgataaaa
    11041 atggacctat ttttcttttg gccgggcggt ggggatgttt agccggttgc taaacgagta
    11101 aaagagaagg aattcatggt gatgaaatga tcaatctgct gttatttcac gctatcaatg
    11161 cacttttggc tgtttttcag gcaaacaaac aaattagggg tttacacgcc gcatcgggat
    11221 gtttatagtg cgcgtcattc cggaagtgtg gccgagcggt tgaaggcacc ggtcttgaaa
    11281 accggcgacc cgaaagggtt ccagagttcg aatctctgcg cttccgccaa ataagataag
    11341 gggttagcta aatgctaacc cctttttctt ttgcctgtcg aaattctcag ggcgttatat
    11401 ttgcttaatg acctgataat ccgctgttaa acctgttcca gatcaaatgc gtaaagatgg
    11461 gtaaaacttc tgggtgccct tacgcattat cattatgctg cttaattaat tacatctgtc
    11521 atagagagtg actcaatgga tcgtattgtt agttcttcac atgaccgtac atcactgctt
    11581 agcacccata aggtgctgcg taatacctat tttctgctga gcctgacgct ggccttttcg
    11641 gcgattaccg caactgccag tacggtgctg atgctgccat ctccgggtct gattctgacg
    11701 ctggtgggta tgtatggttt gatgttcctg acctataaaa cggcgaataa gccgaccggg
    11761 attatctccg cattcgcctt taccggtttt ctgggttata tcctcggacc tattctgaac
    11821 acctatctgt ctgccggaat gggtgacgta atcgctatgg cactgggcgg aacggcgtta
    11881 gtgttcttct gctgctctgc atatgtgctg accacccgca aagatatgtc gttcctcggc
    11941 ggtatgctga tggcgggtat tgtggtggtg ctgattggta tggttgcgaa tatcttcctg
    12001 cagctgcctg ctctgcatct ggcgatcagc gcggtcttca ttctgatctc ctctggcgct
    12061 atcttgtttg aaaccagcaa catcattcat ggcggtgaga cgaactatat tcgtgccacg
    12121 gttagcctgt atgtttcgct gtacaacatc ttcgtcagcc tgctgagcat tctgggcttc
    12181 gctagccgcg attaatctca cccgctaaca ctttcatagc ctcgctttat gcggggcttt
    12241 gttttttgtt acactgccgc cagtctgatt aaggtagtaa taattatgct gatcttcgaa
    12301 ggtaaagaga tagaaacgga taccgaaggc tatctcaaag aaagcagcca gtggagtgag
    12361 ccgctggctg tggtgattgc agagaacgaa gggatttcgc tgtcaccgga acactgggaa
    12421 gtggtgcgtt ttgtgcgtga tttctatctg gaattcaata cttctccggc gattcgtatg
    12481 ctggtaaaag cgatggcgaa taaatttggc gaagaaaaag gtaacagccg ctatttatac
    12541 cgactgtttc cgaaaggtcc ggctaagcaa gccaccaaaa ttgctggcct gccaaaaccg
    12601 gtaaaatgta tttaacggat acgaaaatcg gttaattccc ccgagggatg atgcggctcg
    12661 ctgagcaccc gttctacacg cgcagaacgc gggccgccac tctttaacca ctgcattaat
    12721 ttttccacct gcccttcttc accgcaagca accacttcca cgctgccatc gtcaagattt
    12781 ttggcgtacc cggttaaacc cagtcttttc gcttcgtact gtgtggtgta gcgaaatcct
    12841 acgccctgaa ctcgcccgta aacccaggca attatgcaga ctttcgacat tgctgctctc
    12901 cttatcctta atggacgcgt tgcaaatcca tggaaaactc cggacaatga cgcccatttc
    12961 tttgcatcga cagaaaagta aattatgagt gtacgtttag tgttagccaa agggcgcgaa
    13021 aaatcattac ttcgtcgcca tccgtgggtc ttttccgggg ccgttgcccg catggaaggt
    13081 aaagccagcc tcggtgaaac catcgatatt gttgatcatc agggaaaatg gttagcacgc
    13141 ggcgcttatt cgccagcttc gcaaatccgg gcgcgcgtct ggacgtttga cccgtctgag
    13201 tctatcgaca ttgctttttt ttcccgccgt ttgcaacaag cacaaaaatg gcgtgactgg
    13261 ctggcgcaaa aagatggcct cgacagctat cgtttaatcg ccggagaatc tgatggcctg
    13321 ccgggtatta ctatcgatcg tttcggtaat tttctggtgc tgcaactgct gagtgctggc
    13381 gcagaatatc agcgcgcggc attaattagt gccctgcaaa cgctgtaccc ggaatgtgcg
    13441 atttacgatc gcagcgatgt tgcggtacgt aaaaaagaag ggatggagct gacccagggc
    13501 ctcgtcaccg gcgagttgcc acctgccctg ctgccgattg aagaacacgg catgaaactg
    13561 ctggtggata ttcagcacgg acacaaaacg ggctactacc tggaccagcg tgatagccgc
    13621 ctggctaccc gccgctacgt tgaaaataaa cgtgtgctga actgtttctc ctataccggt
    13681 ggtttcgccg tatcggcact gatgggcggt tgcagccagg ttgtcagcgt tgatacctcc
    13741 caggaagcgc tggatattgc acggcagaac gttgagctga acaaactgga tctgagcaag
    13801 gctgagtttg tccgtgatga tgtctttaaa ttgctgcgta cttatcgcga tcgcggtgaa
    13861 aaatttgacg ttatcgtgat ggacccgccg aagtttgttg agaataaaag ccagttgatg
    13921 ggcgcgtgtc gtggctataa agatatcaac atgctggcga ttcagttgct gaatgaaggc
    13981 ggtattctcc tgactttctc ctgttccggt ctgatgacca gcgatttatt tcagaaaatc
    14041 atcgcggatg ccgcaattga tgccggccgt gatgtacaat ttatagagca gttccgtcag
    14101 gcagccgatc atccggtgat cgctacctac ccggaagggc tatatctgaa agggtttgcc
    14161 tgtcgcgtca tgtaacttga aatgtggaat tttaccctta tatcaaaggt aaagatttcc
    14221 cgggaggtga ctatgattgc cagcaaattc ggtatcggcc agcaggtccg ccattccctg
    14281 ttaggttatc tcggagtggt cgtggatatc gacccggttt attcgctctc tgaaccgtcg
    14341 cctgatgagc tggcggtgaa tgacgagctt cgcgccgctc cgtggtatca cgtggtgatg
    14401 gaggacgata acggcctacc ggttcatacc tacctggctg aagcccagct aagtagcgaa
    14461 ttgcaggatg aacatcctga acagccatcc atggatgagc tggcgcagac catccgcaaa
    14521 caactccagg ccccgcgtct gcgtaactaa tccctgaacg aatccccgga aggtttttgc
    14581 cttccgggga ttttttattt ggccaggccc aggcgaggga tctcaatagc cgggcagcga
    14641 tccatcacca catttaaccc ggcatcccgt gccagtactg ccgcttgctc attaatcacc
    14701 cccagttgca tccagagggt tttcgcgcca atggcaatag cttcctgtgc cacgccccac
    14761 gccgcttctg aattacggaa aacatcgacc atatcgacct tttccggaac gtctgccagc
    14821 gtgccatacc cctgctggcc cagcagcgtt ttgccggcaa cttttggcga aaccggaatg
    14881 acgtgatacc cctggtcgag tagatatttc atcacgcgat agctgggacg atcgggtttg
    14941 tcgcttgccc ccaccagcgc aatggtgtga gtggacgtta aaatgcctgc aatatcggtt
    15001 tctttcatta tttcctccag actgtttaac aaagtgtacg acaaagccga catgccaacc
    15061 atgcagctaa tacgaatgaa tgagagcaaa agcgggaaat atgtctatat gttagtaagt
    15121 atgattaaag aaaaattttg acacattatt attcgggcgg tcttttgaca ggagctacct
    15181 atgaaaaccg gcatcgtgac caccttgatt gcattatgtt tgccggtatc cgtttttgcc
    15241 accacgctgc ggctttcaac cgatgtcgat cttctggtac tcgacggcaa gaaggtttcc
    15301 agctctctgc tgcgcggtgc ggacagcatt gaactggata atggaccgca tcagttagtg
    15361 tttcgcgttg agaagacgat tcatctttcc aatagtgaag aacggctgta tatctcccca
    15421 ccgctggtgg tcagttttaa tacccagctc atcaaccagg tcaattttcg cctgcctcgc
    15481 ctggagaatg agcgggaagc taaccatttt gatgccgcgc cgcgccttga attgttggat
    15541 ggcgatgcga cgccgattcc ggtaaagctg gatattctcg ccattacctc aacggcaaaa
    15601 acgattgatt atgaggtcga ggttgaacgc tataacaaat ccgcgaaacg cgcttcacta
    15661 ccgcaatttg ccacgatgat ggcagatgac agtacgctgc tttcgggtgt ttccgagctg
    15721 gatgctattc caccgcagtc tcaggtgctc acagaacaac ggctgaagta ttggtttaaa
    15781 ctggctgacc cacaaacgcg aaatactttc ctgcagtggg cggaaaaaca accatcttcc
    15841 tgagattttt gtgcctgtgc gcaggctttt tcggtcttta tcttgcagcg ataagtgctt
    15901 acagtaatct gtaggaaagt taactacgga tgtacattat ggaactgacg actcgcactt
    15961 tacctgcgcg gaaacatatt gcgctggtgg cacacgatca ctgcaaacaa atgctgatga
    16021 gctgggtgga acggcatcaa ccgttactgg aacaacacgt actgtatgca acaggcacta
    16081 ccggtaactt aatttcccgc gcgaccggca tgaacgtcaa cgcgatgttg agtggcccaa
    16141 tggggggtga ccagcaggtt ggcgcattga tctcagaagg gaaaattgat gtattgattt
    16201 tcttctggga tccactaaat gccgtgccgc acgatcctga cgtgaaagcc ttgctgcgtc
    16261 tggcgacggt atggaacatt ccggtcgcca ccaacgtggc aacggcagac ttcataatcc
    16321 agtcgccgca tttcaacgac gcggtcgata ttctgatccc cgattatcag cgttatctcg
    16381 cggaccgtct gaagtaatat tgcacaggtg gcaaacgcca cctgtttctt acggttttct
    16441 cgccaccggc acatccagat ttttcagtat ttccacaaag ggagagggat tctctttgtt
    16501 aaacagtgcc catacccgat ggcgtgcccg ggtcagcgcc acgtacatta accgccgttc
    16561 ttccgcgtcc gggaaatcct caaccggtgg cagtagcgcc tcttccataa tcgattcccg
    16621 cgccgcagcc ggaaaaccat cacttccctc ctgcaagcca acgatgatga cgtaatccgc
    16681 ctgttgccct ttgctggcat gaatggtcat aaagtcgatt tgcaacttcg gccagcgtgt
    16741 tgccgctttt tccaggctgg caggcctcat gtgatggtaa cgcgccagga tcagaatgcg
    16801 ctcttccggt ttggcataac cagagagctt atccagcaaa gcgtcaagtt gactctcatc
    16861 caataacgtg acggcttttt tgtctccatt ggttaagctg tttagcggct ttttcaactg
    16921 gcctgggttc tgctgaataa accggtttgc cacctcaccg atacgactgt taaaacggta
    16981 agtcgtgtct aaatcacagc gatcgccttc accaaagttt tcatggaaag cggtggtgag
    17041 cgacatttgc gcaccgctga atcggtaaat cgcctgccag tcatcaccaa cggcgaacaa
    17101 cgtcgtctga ctgttttgct tgcgtaatgc cgctaacaac gctgcccgct gcggcgagat
    17161 atcctgaaat tcatcaacca gaatatgctt ccacgggctg ataaagcgac ctttctccag
    17221 aatcacaatc gcctgatgaa taaggcccga aaaatcgaca gcgttttctg ccttcagcgc
    17281 acctttccag gcttttaata acggggccat caacttgata cgtttactga acagatcgcg
    17341 aatctcttcg ggtgcactgg caatcatttc tgcctgtgca ccaccgtgca tccgcatcag
    17401 acttacccaa cgatcgaggc gtgaggcaag acgacgctgt aatttttcat catcccagaa
    17461 gttaccttct ggcactgacc actgcatttc ttccgtcagc cattgccgcc agcccttcgc
    17521 ctgcgctttc ttttcgctgc attgcttgcg ccactcagca ataaagagtt catgacgggc
    17581 agcggtatca ttttccagtt tactgactat cggaactttt ttgctgccct gctgaataat
    17641 atgcagcgcc agagcatgaa acgtgcgtgc ggtaatgtct tcggtatgta gccgttcgcg
    17701 aatacgctcg tccatctctt cagcggcttt gcgaccaaac gccagcaata aaatttgctc
    17761 aggggacgct tcaccacgcg ccagcaacca gcctgcacgg gccaccagca ccgacgtttt
    17821 tccgcttcct gcacctgcca gcactaacag agaatgctcg ccattaacga ctgcccgggc
    17881 ctgcgccgga ttcagcggtg aagactcgac ctggcggaaa aaatccgcat actcggtaag
    17941 catggcttcg gtatacgcct ggttatgctg caaccgagcg ctttcaatat ctttcaacca
    18001 ggcctgacat ttacgccacg cctcacggca gttatcgaat tcttccagtc ggttaaccgg
    18061 caacggcaac gccgacaaag cctggcggat ttgttgctga acgccagagg tttgctcacg
    18121 cgtcagccat ttattttctc cagtgcgcgt ggcaatcaaa tccagttgct ggcgtaaaac
    18181 accagacgca atttcgctca tctcgccact ccaccgccgc cagtgagcat caagatgatg
    18241 gtaaaaacgc tgcgtctcgc cccattcggt gccgtgcaga cgcaccactt tttcgtccgg
    18301 cagtacaaat tccagctcgc cccataccag accgcgctta cagtgaatcg ccagtaattg
    18361 attgaaagga ataaggtatt cgtggcgatc gccggagact ttaatcccgg cattgaggat
    18421 caccgcccga tcgtaagggt gctgtgccag acgttttcca agcgttgtcg ctttcagttc
    18481 catgactctg tagatccaca cgaaattatt tcttatcagt gtaaccgtca gagaaaacgt
    18541 gctccagccc aaaaaaacgt tacaattgcc gcccattact gaaaaccaca gtaaagcgag
    18601 gttttatgcg taccgttttg aacattctga actttgtgct tggcggattt gccaccactc
    18661 tgggctggct gttggcgact ctggtcagta ttgtgctgat ttttacctta ccgctgacac
    18721 gatcctgctg ggagatcact aaactgtctc tggtgcctta tggcaatgaa gctattcatg
    18781 tcgatgaact gaacccggct ggcaaaaatg tgctgctgaa tactggcggt acggtattga
    18841 atattttctg gctgattttc tttggctggt ggttatgcct gatgcacatt gcaacgggca
    18901 tcgcacaatg tatttcaatc attggcattc ctgtcggcat tgcgaacttt aaaattgccg
    18961 ctattgcact atggccggtt ggtcgtcgcg tggtatcggt agaaacagcg caagctgcgc
    19021 gtgaagccaa tgcacgtcgt cgttttgaat aatccactaa tatggccttt atgctaagtc
    19081 ctttgctcaa acgctatacc tggaacagcg cctggctgta ttacgcgcgt atttttattg
    19141 cactttgtgg aaccacagcg tttccgtggt ggctgggtga tgtaaaactg acgattccgc
    19201 taacgctggg gatggtggca gcggcgctga ccgatctcga tgaccgactg gcgggacgtt
    19261 tgcgtaacct catcattacg ctgttctgct tttttatcgc ctcggcctca gtagaattgc
    19321 tgtttccctg gccctggcta tttgcgattg gcttaacgct ctctaccagc ggcttcattt
    19381 tgctcggcgg tctgggtcaa cgctatgcaa caattgcctt cggtgcattg ctgatcgcca
    19441 tttacactat gttgggaaca tcactgtatg agcactggta tcagcagccg atgtatctgc
    19501 tggccggtgc cgtctggtac aacgtcctga cacttattgg tcatctgctg ttcccggtcc
    19561 gcccgctgca ggacaacctg gcgcgttgct atgaacaact ggcgcgttat cttgagctca
    19621 agtcgcgcat gtttgatcct gatattgaag atgaaagcca ggcaccgctg tacgatttgg
    19681 ctctcgccaa cggtctgctg atggcgacat tgaatcagac gaaactctcg ctgctgaccc
    19741 gcttacgtgg cgatcgtggt caacggggaa cgcgtcgcac gctgcattat tactttgtcg
    19801 cacaggatat tcacgagcgt gccagctctt ctcatattca gtatcaaaca ttgcgtgaac
    19861 attttcgcca cagcgacgtg ctgttccgtt ttcagcggct gatgtcgatg cagggccagg
    19921 cgtgccagca actgtcacgc tgtattttgt tgcgtcagcc ttatcaacat gatccgcatt
    19981 ttgagcgcgc ttttacgcat attgatgctg cgctggagcg gatgcgcgat aacggcgcac
    20041 ccgccgattt actcaaaaca ctgggatttt tgctgaacaa tttacgcgcc attgatgccc
    20101 aactggcaac aattgaatca gaacaggccc aggcactacc ccataataat gacgaaaatg
    20161 agctcgctga tgacagcccg cacgggttga gtgatatctg gctgcgtctt agccgtcact
    20221 tcacgccgga atccgccctc ttccgtcatg cggtaagaat gtcgctggtg ttgtgcttcg
    20281 gctacgccat cattcagata accggaatgc atcacgggta ttggatcttg ctgacaagtt
    20341 tgtttgtctg ccagccaaac tataacgcca cgcgccaccg cctgaagtta aggattattg
    20401 gtacgctggt aggtatcgcc attggcattc ctgtgctgtg gtttgtgcca tcactggaag
    20461 ggcagctggt gctgctggtt attaccggcg tgctcttttt tgccttccgt aacgtgcaat
    20521 acgctcatgc aacgatgttc atcacacttt tggtgctact gtgttttaac ttactgggtg
    20581 aaggttttga agtagcgtta cctcgcgtaa tcgatacgct gattggttgt gccattgcgt
    20641 gggcggcagt gagctacatc tggcctgact ggcagtttcg caatctgccg cgcatgctcg
    20701 aacgcgccac agaggccaac tgtcggtatc tcgatgccat actggagcaa taccatcagg
    20761 ggcgtgataa ccgtctggcg tatcgtattg cccgccgcga tgcacacaac cgtgatgctg
    20821 agctggcgtc ggtggtatca aatatgtcca gcgagccgaa cgttaccccg caaattcgcg
    20881 aagccgcgtt tcggttgctg tgccttaacc atacgtttac cagctatatc tcagccctcg
    20941 gtgctcaccg ggagcagtta actaatcctg aaattctggc gtttcttgat gacgcagttt
    21001 gctatgttga tgacgcgtta catcatcaac ctgctgatga agaacgcgtc aatgaggcat
    21061 tagctagcct gaaacagcgg atgcagcaac ttgaaccacg ggcagacagc aaagaacctc
    21121 tggtcgtaca acaagttgga ttattgattg cattactgcc tgagattggt cgtctgcaac
    21181 gccagattac tcaagttccg caggaaactc ctgtttcggc gtaagagagt cagcccattc
    21241 tgcaagctcc tggcggcgtg ccaccgggag cgcagcttca tgaatgccga taatggcacc
    21301 ttcaagcata aacagaatct tttctgtcac cagactgttt tgctgccgca gtcgcaacca
    21361 acacattttt gccccaagta tacgtaacgc ccgtacatcc ttaatcccca cctccccgag
    21421 aatcgcttcc agatgaaaag acatattggg caaatctttc agtctttccc gggtattgcg
    21481 cgtgcttttc tctttcagcg ctgcatcgag agaatatttc gacagacgca ccagcttcag
    21541 ttgatttcgc catagacttt catcaacccg atagtaattg agggtaacgg atcggccaca
    21601 ctttttatat gtcagccaga caggcggatg ttttacacag tactgtgcac tttgctcaca
    21661 agcccgaaga tacaactcac catcagaaac catcgcaaac accgtgtcgt caacggtcag
    21721 gctgtaactg ccaaacaatg atcggtattc aattgtgccc aacgttgcca ggtattcttg
    21781 tgatttatag atccgcttat aggagaggct tttcataaaa ttccttttaa aatcataaca
    21841 taaaagaatg attcacatta acggatccgt taactacgaa aataggcaac ttattcttaa
    21901 ggggcaagat taatttatgt tttcccgtca ccaacgacaa aatttgcgag gctctttccg
    21961 aaaatagggt tgatctttgt tgtcactgga tgtactgtac atccatacag taactcacag
    22021 gggctggatt gattatgtac acttcaggct atgcacatcg ttcttcgtcg ttctcatccg
    22081 cagcaagtaa aattgcgcgt gtctctacgg aaaacactac agccgggctt atcagtgaag
    22141 ttgtctatcg cgaagatcag cccatgatga cgcaacttct actgttgcca ttgttacagc
    22201 aactcggtca gcaatcgcgc tggcaactct ggttaacacc gcaacaaaaa ctgagtcggg
    22261 aatgggttca ggcatctggg ctacccttaa cgaaagtaat gcagattagc cagctctccc
    22321 cttgccacac cgtggagtca atggttcgcg ctttacgtac gggcaattac agtgtggtga
    22381 tcggttggtt ggcagatgat ttaactgaag aagagcatgc tgaacttgtt gatgcggcaa
    22441 atgaaggtaa cgctatggga tttattatgc gtccggtaag tgcatcctct cacgccacga
    22501 gacaactttc cgggctaaaa attcactcta atttgtatca ttaagtaaat ttaggattaa
    22561 tcctggaact ttttttgtcg tccagccaat gctttcagtc gtgattaatt ttccttgcgg
    22621 aggcttgtct gaagcggttt ccgcgatttt cttctgtaaa ttgtcgctga caaaaaagat
    22681 taaacatacc ttatacaaga cttttttttc atatgcctga cggagttcac acttgtaagt
    22741 tttcaactac gttgtagact ttacatcgcc aggggtgctc ggcataagcc gaagatatcg
    22801 gtagagttaa tattgagcag atcccccggt gaaggattta accgtgttat ctcgttggag
    22861 atattcatgg cgtattttgg atgataacga ggcgcaaaaa atgaaaaaga cagctatcgc
    22921 gattgcagtg gcactggctg gtttcgctac cgtagcgcag gccgctccga aagataacac
    22981 ctggtacact ggtgctaaac tgggctggtc ccagtaccat gacactggtt ttattcctaa
    23041 caatggtccg acccacgaaa accaactggg tgcaggtgct tttggtggtt accaggttaa
    23101 cccgtatgtt ggctttgaaa tgggttacga ctggttaggt cgtatgccgt acaaaggcga
    23161 caacatcaac ggcgcataca aagctcaggg cgttcagctg accgctaaac tgggttaccc
    23221 aatcactgac gatctggaca tctacactcg tctgggtggt atggtatggc gtgcagacac
    23281 caaggctaac gtacctggtg gcgcatcctt taaagaccac gacactggcg tttctccggt
    23341 tttcgcaggt ggtgttgagt acgcgatcac tcctgaaatc gctacccgtc tggaatacca
    23401 gtggaccaac aacatcggtg acgcaaacac cattggtact cgtccggaca acggtctgct
    23461 gagcctgggt gtttcctacc gtttcggtca gggcgaagca gctccggtag ttgctccggc
    23521 tccagctccg gcaccggaag tacagaccaa gcacttcact ctgaagtctg acgttctgtt
    23581 caacttcaac aaagcaaccc tgaaaccgga aggtcaggct gctctggatc agctgtacag
    23641 ccagctgagc aacctggatc cgaaagacgg ttccgtagtt gttctgggtt acactgaccg
    23701 catcggttct gacgcttaca accagggtct gtccgagcgc cgtgctcagt ctgttgttga
    23761 ttacctgatc tccaaaggta tcccggcaga caagatctcc gcacgtggta tgggcgaatc
    23821 caacccggtt actggcaaca cctgtgacaa cgtgaaacag cgtgctgcac tgatcgactg
    23881 cctggctccg gatcgtcgcg tagagatcga agttaaaggc atcaaagacg ttgtaactca
    23941 gccgcaggct taagttctcg tctggtagaa aaaccccgca gctgcggggt ttttttcgcc
    24001 tttagtaaat tgaactgact tcgtcagtta ttccttaccc agcaatgcct gcagatcatg
    24061 cttcaaagaa gacattttat tcgcgtattt ctctttgttt tccgcatctt caatcagctg
    24121 aacaatcgtt tcagaaagcg ttttaccgcg acgctgcgca agaccagcca gacgttgcca
    24181 gacaataaat tccagatcga tcgatttttt gcgcgtatgc tggtgttccg cattaaaatg
    24241 gcgcttccgt ctggcccgaa tggtttgctt catgcgattg accagttccg gattcatatg
    24301 cttgtcaatc cagccattta ccagcacggg ttcattttcc agcgagagca acacatcgac
    24361 ggcttcctgg gcggcactgg cttctatgta acgggtgatt aactcccctt cgcgatgctt
    24421 cttcaccaga tacttccatt tccaaccgct ttcaagattt tcaagttgtt gatatttcat
    24481 agcgatctca atgttaccgt gtaactcttt acagaatatc agctttttag gccagtgaag
    24541 aaaagaatct ccatcctgtg agctttaacg cccatgccag gagtattgtc ggtgctttct
    24601 acgtgtgctg tagtgccggt tacggtataa tcgcggcttt gacaacagac taaaaaacat
    24661 caactttgac cattacgaaa cttgcatggc gtgacctggt tcctgatacc gatagctatc
    24721 aggaaatatt tgctcagcca catttgattg acgaaaacga tcctttattc agtgatactc
    24781 aaccgcgact gcaattcgca ctggagcagt tgctgcatac gcgagcatcc tcctctttta
    24841 tgctggcgaa ggccccggaa gagtctgagt atctgaatct tattgccaat gccgcgcgta
    24901 cgctacaaag cgatgcaggc caactggtgg gcggtcacta tgaggtttcc ggccactcca
    24961 tccgcttacg tcacgcagtg agtgcagatg ataattttgc gactttaacg caagttgtcg
    25021 ctgccgactg ggtagaagcg gagcaactct ttggctgcct gcgccagttt aatggcgaca
    25081 ttaccctgca gcctggtctg gtgcatcagg caaatggcgg tattctcatc atctctttgc
    25141 gtacactgct ggcgcaacct ctgctgtgga tgcggctgaa aaatatcgtt aaccgcgagc
    25201 gttttgactg ggttgcgttt gatgagtcgc gccctctccc cgtctctgtg ccttcgatgc
    25261 cattgaagct gaaagtcatt ctggtaggcg aacgcgaatc attggctgat ttccaggaga
    25321 tggagccaga gctttcagag caggctattt atagcgaatt tgaagatact ctgcagattg
    25381 tcgatgcgga gtcagtaagc cagtggtgtc gctgggtgac atttaccgcc agacataatc
    25441 acttacctgc cccgggagcg gatgcctggc cggtacttat ccgcgaagca gcacgctaca
    25501 ccggtgaaca agaaacactt ccgcttagcc cgcagtggat cctccgccag tgtaaagagg
    25561 tcgcctccct gtgtgatggc gacaccttct ccggcgagca gctaaactta atgctgcagc
    25621 agcgtgaatg gcgcgaaggt ttcctcgctg aacgtatgca ggatgagatc cttcaggagc
    25681 aaatcctgat tgaaaccgaa ggcgaacgca tcgggcaaat taacgccctt tcggtcattg
    25741 aatttccggg tcatccacgc gcttttggcg aaccttctcg cattagctgc gttgtgcata
    25801 ttggcgatgg tgaattcacc gacatcgaac gcaaagcgga gcttggcggc aatatccatg
    25861 cgaaagggat gatgatcatg caagcgttcc tgatgtcgga actacagctt gagcaacaga
    25921 tccccttctc agcatcgctg acatttgagc agtcatacag tgaagttgat ggagatagtg
    25981 cctcgatggc tgaactctgc gccctgataa gcgccctcgc cgatgtgccg gtgaatcaga
    26041 gtatcgctat cacaggttca gtcgatcagt tcggtcgcgc ccagccggtc ggtggtttaa
    26101 atgagaaaat cgaaggcttc tttgctattt gccagcaacg tgagttaacc gggaaacaag
    26161 gtgtcattat ccccacagct aacgttcgcc atttaagtct tcacagtgaa ctggtgaaag
    26221 cggtagaaga aggcaaattc accatctggg cagtagacga tgtgactgac gtactgccgt
    26281 tattattaaa tctggtgtgg gatggcgaag gccaaacgac gctgatgcaa accatccagg
    26341 aacgtatcgc gcaagcatcg caacaggaag gacgtcaccg ttttccatgg ccattacgtt
    26401 ggctgaactg gtttattccg aactgatcgg acttgttcag cgtacacgtg ttagctatcc
    26461 tgcgtgcttc aataaaataa ggcttacaga gaacatggta gataaacgcg aatcctatac
    26521 aaaagaagac cttcttgcct ctggtcgcgg tgaactgttt ggcgctaaag gcccgcaatt
    26581 gccagcaccg aacatgctga tgatggaccg tgtggtcaaa atgaccgaaa cgggtggtaa
    26641 cttcgacaaa gggtatgttg aagcagaact ggatatcaat ccggatctgt ggttcttcgg
    26701 atgccacttt attggcgatc cggttatgcc gggatgcctg ggcctggacg caatgtggca
    26761 gctggtaggg ttctacctcg gctggctggg cggcgaaggt aaaggccgcg cgctgggcgt
    26821 tggcgaagtg aaattcactg gtcaggtact gccgacagcg aaaaaagtga cctaccgtat
    26881 tcactttaaa cgcattgtta atcgtcgtct gattatgggt ctggcagatg gcgaagtgct
    26941 ggttgatggt cgtctgatct ataccgccag cgacctgaaa gtcggtctgt tccaggatac
    27001 gtctgccttc tgattataat cagacagagt atcaaaaggc gaaacctccg caatgcggag
    27061 gtttcttttt aaagagacag aatcaggcca ttactaccct gtccgccatg gcttctcgcc
    27121 agcctcccag ccattgtgac ctttgattca gcgtctgata gggacacatt tcttttgagc
    27181 gtccggcgat gccggcctga taaccacgtt gatgtgcccg ttccaggcga tctcgttttt
    27241 gtctcttcat gcctcgtttc cctcatactg gtttctggtg gaaaagaaaa cagtgattac
    27301 taaatgtgca atcacagtta agcaataccg cgaatcataa ctgccgtcaa tgcgcaaaat
    27361 tcacgccact gtcatatttg tgagctacac gggtatgtcg gttgtacaaa aaatgtgaag
    27421 aggaacaact ttttccgcac gttagagaca aaaaaaccgc ctcaggcaac ataccggagg
    27481 cggttatatt tacaaatctt tataattaag gtagacgctt tatctcttgc gcaatagaag
    27541 cggcttcctg actccagaca ctggccagta ctttaaccat ctcatcgtaa ccatcctgag
    27601 tttgcactcc ttccagacgg aacggacgtt tgatcagttg tccctggtgg ttcaacagcc
    27661 actcaccact gacaatgacc ttgccatcat agcgaccgtt aaactccgtt acggtaacat
    27721 tgagcgtgtc ctgggcgctt cccagaggct gggaggcaac cacccagccg ggcagttgcg
    27781 tactcaggtt ggcaaccagg gtgttgcgca actgttgatc caacgggctg gcccacaagt
    27841 tgttgttggc aatcacatac ttcacatcac tggtttgata aaccacacca ttccccgcca
    27901 gatagtcagg aacagtgacc tgctctaccc ataacagacg attgccctgg ctggcggtac
    27961 tttgtgtacc gccctgcacc acaggtaact ggtaatagtt tttattaatt tcgccggagc
    28021 tgcatccggc cagccacagt gctgcaatcg tcactagcca ctttttcatt gtttcgccct
    28081 cttcggctct ggatcttttt tgtccttcgc ttcaaatacc agcgcgttac tcttctcatt
    28141 gagcgttttc agcaccggtt gcagttctcg caacacctga tcaaggcgct gcatatccgc
    28201 caccatcttg ttgtaggctg cggagccagg ctggaagccc tgcatgctgc gattcaattc
    28261 acgcaacgtt gactgcatat ccgtcggcaa ctgctgcatc gactggctgg cgaggatctt
    28321 gttcatgcta tccagcgtcg tttgcaggtt tttcattgtg cgctgacttt cagaaagcgt
    28381 actggttgcc tgttcaatca tcggattcaa tggcagtttg ttgatcttat ccaacgcttc
    28441 catcagtcgt tgctggattt gcgccaggcc gccgctaacg gtcggtataa tctgataacc
    28501 attaaattca cgaataccgg ttattgcagg cgtatttgga tagaaatcga gatcaacata
    28561 tagtgcgcca gtgaccaggt ttccggtttt cagcgatccg cgtaaaccac gtttcaacaa
    28621 ttcgccaagg tgctcaacaa catccgcatt ttcgccaagc tgcattttca gccgctctgg
    28681 ctcgatacga atcagtaccg gaatacggta atcatcgtta aatgtctgac gcatattcgg
    28741 cgcaaagaat ggcactttgc ttacggtacc caggcgaata ccccggaatt ctaccggagc
    28801 tcccggttgc agaccgcgta ccgaatcttt aaagaacatc agataatcaa tgtgatcggt
    28861 gtacaacgaa tcctgaatgc tcttctgatc atcatacaaa acgaaagctg ttttcggtgc
    28921 cactggctgc cctaaatcca gaccttccgg cacatcaaag ctgacaccgc cactcagcag
    28981 cgttgtcaat gagcccatct ccacacgcat ccctgctgac gtcagatcaa ccgcaatgcc
    29041 actatctttc cagaagcgaa cattgttggt caccagtcgg tcataaggtg cattgatgaa
    29101 cagttgatag ctgatattgc gtttttgtgt atcgaaggtg ctggtttcaa ccgaacctac
    29161 ccgatagcca cggaacagca ccggatctcc tggcgagagc tgcccggctt ttttgctatc
    29221 gagaatcaca cggatacctt tcgcatcagg cggggccaac ggtggcgagt ccagcaaatc
    29281 gtatttatcc attttgctgc ctttcgcgcc tggctgcagt tcgatataaa ctccagacag
    29341 cagcgttccc aggccgctaa tcccttcgcg accaatctgc ggtttcacca cccaaaagac
    29401 ggtgtcttta tgcagcaatt tttccatacc ggaattcagc cgcgctttga tttcaacgtg
    29461 cgtcaaatca tcagccagtg tggcgctttc aaccacgccg acgtcaacgc tacggctttt
    29521 aatggtggtt ttgccacctt caattccttc cgcattcgcg gtgatcaggg tcacttccgg
    29581 tccctgatgg ctgtaatgat aaaaaagaac ccaggcccca atgagcgccg tgacgatagg
    29641 aaatatccac acgggagacc agttcttcac tttctggatt ttggcttccc cattattaga
    29701 ttccatactg tcaggactcc tcatgctctg attcaggttg acgatcccac gacaaacgcg
    29761 ggtcaaacgt catagcagaa aacattgtca ttatgacgac taaagcaaac attaatgcac
    29821 ccattgccgg ataaatactc attaaacctc ccatacgcac cagcgccgag agcaccgcga
    29881 taacgaaaac gtcaatcatc gaccagcggc ctacaaactc aacaacttca taaatcaaat
    29941 gcattctttc actgtcgcgc ttgccatgcc ctttggcatc ccaacacagc cacgcgatgg
    30001 cgatcatctt taacgttggc accataatac tggccagaaa gatcaccgca gcgacgggat
    30061 aagagccttc gctccataac aggatgaccc cagcgagaat cgtcgatggc atcttcgagc
    30121 ctaataaatc cgtcaccatg atgggcaaaa tattagccgg aagatacagc atgatggacg
    30181 taacaagcag cgcgagtgtc cattgcaggc tgtttctacg ccgaacgtac cctttggtac
    30241 tacaacgcgg gcacacgggt tcatcagcag gaaggattgc cgtacaacag gagcatgaac
    30301 gcagcccctg acgtatcccc gtgacgcctg gttttagcgg ctggcgcagt tctggcatcg
    30361 gggcgatatc gtcccataac cagcgacgat caacgcactg aaaagcacgc agttgcagga
    30421 cacaaaataa acaccagggg agaaagctgc tgcctacgcc aatgctgccg taagccatca
    30481 gtttaacgaa actgaccagc acacccgcga ggaaaatctc cgccattccc caggttttga
    30541 gttgaaaaag cacccgtgcc agttgctctt ttaaacggac cggtaattcc gcgcgattca
    30601 ccagtaacag aatggttatc agacaaaacg ctggaaccag ttgcacaaac aacaggaaaa
    30661 aggtgccgag gctggcgtag tcctcagaaa aaagcacgcc gggaatttcc agtaatgtta
    30721 tttcactggt aactcctgca acgttcatat tcacaaaagg aaacaagttg gacagcaaca
    30781 gcatgaacag tgcagccaac gcataggcgg tcggacgctg ccgaggggca tcccacgcca
    30841 cggttaacgt tgtgccacac cggggacatg ccgctttctg accatgctca aggcgcggta
    30901 acgccaccag catgtcacac tgcgagcaca ggatgtgctt cgcggcatga tgatgttcgc
    30961 acattggtac tccttaatga tacgctattt tacagatgac taacaaagtg ttggttttca
    31021 tgcctgataa acccagttca tcaggcaatt ttaccgttgt cagctgtctc tgtttaaatc
    31081 gactattttg cgatcagcca ccatttttta acgcttcaag atactcccag cgttcaaagg
    31141 cttgctccag ctcctgctct gcagcagcca tatcggcgag cactttttgt gtctgctcat
    31201 gcggctgact gaagaaggaa gcatccgcca cctgcgtctg tagggcttcc agcttcgcct
    31261 ccagatcttc gagcaattgc ggtagctgct ccagttcgcg ctgcaattta tagcttagtt
    31321 tgctactgct gcgttttaca gtttctgctt ttggcacggc ggcttcttcg gtttttttca
    31381 ccgcaggctg tttgagcgcc acatactgtt cttgctgacc acgggcatca tgataaccgc
    31441 caacataacg accaatttta ccgccgcctt cgaagatcca gcattctgta acggtgttat
    31501 cgacaaactg acgatcgtgg ctaaccagca ataccgtgcc ctgatagctg tcgatcagtt
    31561 cttccagcag ttccagcgtt tcgacatcaa gatcgttggt cggttcgtca agaatcaata
    31621 agttgcttgg tttgaggaac aaacgcgcca acaacaagcg gttacgctca ccgccagaaa
    31681 gcgcacgtac cggcgtcatc gcccgttttg gatggaacag gaagtcctgc aaatagccca
    31741 atacgtggcg tggcttgccg ttaaccatca cctcttgctt accttcggca aggttatcca
    31801 tcaccgtttt atcgggatcc agttccgcgc ggtgctgatc gaaataagcc acttccagtt
    31861 tggtgccaac gtgaatacgc ccgctgtctg cttgaagctg accgagcatt agtttcagca
    31921 gcgtggtttt gccgcaccca ttcggaccaa tcagggcaat tttgtcgcca cgtagaacct
    31981 gggcagaaaa atctttcacc agttgcttac cgtcaacctg atagcaaacg tcttccattt
    32041 cgaaaacgat cttaccggag cggctggcct cttccacctg catctttgcg gtacccatca
    32101 cttcgcgacg ttcgccccgc tcgcgacgca tcgctttcag ggcgcgtacg cggccttcat
    32161 tacgggtacg gcgtgctttg atcccctggc gaatccacac ttcttcctgc gccagtttgc
    32221 gatcgaactc ggcattttgt agttcttcca cgcgcagggc ttcttctttt tccagcaggt
    32281 actgatcgta attgcccgga taggtcacca gcttgccgcg atcgagatca acaatgcgcg
    32341 tcgccatatt gcggataaac gaacggtcgt gggagatgaa aataatcgtc ccattgaagg
    32401 ttttgaggaa cccttccagc cagtcgatgg tttcgatatc caggtggtta gtcggttcgt
    32461 ccagcagcaa tacgcgcgga ttgctcacca gcgcgcgtcc taatgccgct ttacgcaacc
    32521 agccgccgga aagcgacgac agcgcaacgt taggatctaa ccccagttgc gccagcactt
    32581 cgttgatgcg gttttccagc tgccacaggt tgtggtgatc cagctgttcc tgaacctttg
    32641 ccagttcgtt gagatttttc tcgctcgggt cgttcatcac caggcgagaa atatcgtgat
    32701 agcgtttcag atattccgct tgttcttcaa tgccttcggc aacgaaatca taaacactac
    32761 cctcaacgtt acgcggcgga tcctgttgca gacgcgctac aatcaaatcc tgctcgtaaa
    32821 taatgcgacc gtcatccagc ccttgttcac ggttgaggat tttcattaac gtcgatttgc
    32881 ctgcgccgtt gcggcccacc agacaaacac gttcgttatc ttcgatatgc agttctgcgt
    32941 tatcgagaag cggcgcgtcg ctgaacgaca gccatgcgcc atgcatactg attaatgaca
    33001 ttactattcc tttcaggctg cggtaatcag ccagcagttg tggatctggc ggttacgggc
    33061 gaaatcctgg gagagcgttt tttgcgtaat ttcttgtgct ttcagtccca gttttgccag
    33121 gccgtcgaga tccatacgga agccacgttt gttgttcgag aacatgatcg tcccaccagc
    33181 acgcagcaga cgtttcaaat ctttcatcag cgccagatga tcgcgctgaa catcaaacgc
    33241 atcttccatt cgttttgagt tagagaaggt tggcggatcg ataaagatca gatcgaactg
    33301 ttcatttgcc tcacgcagcc acgccaggca atcggcctga atcaggcgat gcgcacgccc
    33361 ggttaggcca ttcagacgca ggttgcgttc tgcccactcc agataagtac gcgacatatc
    33421 cacggtggtg gtgctgcgtg caccgcctaa tcccgcgtgc acggtggcgc tgccggtata
    33481 agagaacagg ttgaggaaat ctttgccttt gctcatctga ccgagcatac gacgggcgat
    33541 acggtgatcg aggaacagac cggtatcgag gtaatccgtc aggttcaccc acaagtgagc
    33601 gttatattcg gtgacttcga gaaactcgcc cttctcgccc agtttctggt actgattttt
    33661 gcccttctgg cgttcacggg ttttcagcac cagtttgttt ggcgcaatcc ccagtaccga
    33721 aatggttgca gcgataatat cgaacagacg ctgacgcgct ttgtgcgcat caatagtttt
    33781 tggcggcgca tactcctgca ccaccaccca gtcggcataa cggtcaacgg caacgttata
    33841 ttccggcaga tcggcgtcat acaggcggta acattcaatc ccttcctggc gagcccactt
    33901 ctcgaatttt ttgaggttct tacgcaggcg gttagcgtag tcttccgcta ccatcgccgg
    33961 tttgctgtct ggagtgcttt cggcaacgtg gtaatttttc tgtacgcagt ccagcgggcc
    34021 gtttttcgcc ttgtactgtt tgtctgcacg cagctgcagg cagctgagca gatccggcga
    34081 ggcactaaac aaagagagat tccagccacc gaactggttt ttcatgattc ggcccagcag
    34141 actatgcagc gctatcagcg ccggttcgct gtccagacgt tcaccgtatg gcgggttgct
    34201 caacactgta ccgtacggcc ctttcggcag cggattggtc agttgcgcga catctttcac
    34261 ctcaaaggtg atcagttcac caatccccgc aagacgggcg ttagtgcgcg cacgttgaat
    34321 cacccgtgcg tcgctgtccg aaccgtaaaa atgagagcta tactcagcca ggcctttacg
    34381 ggcgcgagtt tgcgcttccg ctttcacttc ctgccagata gcttcatcat gctgcgccca
    34441 gccgctaaag ccccaacgcc cacggtgcaa gccaggtgcg cgatcggtcg ccagcatcgc
    34501 tgcttcaatc agcaacgtac cggaaccaca catcggatcg agcagcggtg ttcctggctg
    34561 ccagccggat cgcatcacaa tagcggctgc cagggtttct ttgatcggcg caataccagc
    34621 acgatcgcga tagccacgca gatgtaaacc atcaccactg agatcaaggg cgatactggc
    34681 ggtttcttta tgcagccaga cgttaacgcg gatatccggc gcatcgcgat caacatttgg
    34741 acgcggcaga tttttccgcg tgaaagcatc gacgatcgcg tctttcactt tcatcgcgcc
    34801 gtactgactg ttgcggatgg tgtcattcaa accactgaag tggacagcga aggtcgcgcc
    34861 aggattaaac atctctgtcc agttgatcgc ctgaacaccg agatagaggt ctaaatcgct
    34921 gtaaacctta cactcgccca gcggcaacat aatacgcgag gccaggcggc tccacatcag
    34981 gctctggtaa acaagccgtg tgtcgccctt gaaatggacc ccaccctgaa ccacctggca
    35041 ttcaacggcc cccaggtttt ccagttcagt ttttaacagc tcttccagcc cacgggccgt
    35101 actggcaaac agagaattca tatcgtcact tttactctaa gagcctaccg cagtaggcgt
    35161 aattgttgca gtcagtctgg acatggacag cgcggagaaa ccggagcgta cacaagtacg
    35221 tgaggatttc gagcataacc cagggccaaa ctgacaaata aaatagccta atgggacagg
    35281 cgctaagaaa attgttgcgc attatagcta ataagcgcgt catgtcataa agttgagggc
    35341 ttattttcat ttgaggaccg caccgtggcg acattaatcc ggctttttat tcatcctgtt
    35401 aaatcgatgc gcggcattgg tcttacacat gctctggcag atgtcagtgg tctggccttc
    35461 gatcgcatct ttatgatcac ggaacctgac ggtacgttta ttaccgctcg ccagtttccc
    35521 cagatggtac ggtttactcc ttcacctgtg catgatggct tgcatctcac cgcaccagat
    35581 ggcagtagcg catatgttcg ttttgctgat ttcgccacac aagacgcacc aaccgaagtt
    35641 tggggcacac attttaccgc gcgaattgcg ccagacgcga tcaacaaatg gctaagtgga
    35701 tttttctccc gcgaagtgca attacgctgg gtggggccac aaatgacccg gcgcgtgaaa
    35761 cgccacaaca ctgtacctct gtcatttgct gatggctatc cttaccttct tgctaacgaa
    35821 gcctcgttac gtgatctcca acaacgttgt ccggccagtg taaaaatgga gcaattccgc
    35881 cccaatctgg tggtttccgg cgcgtcagcc tgggaagaag atcgctggaa agtgattcgc
    35941 attggtgatg tggtgtttga tgtggttaaa ccttgtagcc gctgtatttt caccaccgtc
    36001 agcccagaaa aagggcaaaa acatccggca ggcgaaccat taaaaacatt gcaatctttc
    36061 cgcactgccc aggataacgg cgatgtcgat tttggtcaga atttaattgc ccgtaatagc
    36121 ggcgtgattc gcgttggcga tgaggtggaa attctggcaa cggctccggc aaaaatttac
    36181 ggcgcagctg ccgctgatga taccgccaac atcacgcaac aaccggacgc aaatgtagat
    36241 attgactggc agggacaggc atttcgtgga aataaccaac aggtgttgct ggagcaatta
    36301 gaaaatcagg gaattcgtat cccttattct tgccgcgcgg gcatttgtgg aagttgccgt
    36361 gttcagcttt tagaaggcga agtcacgccg ctgaaaaaat cagcaatggg cgatgatggc
    36421 accattcttt gctgtagctg tgtaccgaag actgcactta agttggcgcg ttagactgcc
    36481 tgttcgaggc tgaagctgtc aacattaacc tgcggtttca gcctgtcgtt catgatttta
    36541 atggcgtcgc ccaactgcat cgcgcgaccc gcaataacaa cgcagggttg tgccagcagg
    36601 catagtgcgg cattttcgcc agattcaacg accagcaaat tgacctgctc gtgagtatcg
    36661 ctcaaccaaa cgcaggccgc atcgcccggc atgggcaccc acatttcacc atgggaaaca
    36721 aaatgccagc ttttcggcat ttgcggtttg agatagcgaa ttgctaccag tgcattcagc
    36781 accagttcgg ctttttgttc tttagataaa ttaaaatcgc ggcacttttc ttcaaaagag
    36841 aaatagagcg cggcgtcgtc aacgcaaaag cctgcgggag aaaaggcatc tggagtcaac
    36901 atcttgcgcg caaaacgtga gcgaaatagc ataccattgg ctaaatcgag catcattcga
    36961 tcgtgctctt catcgtaata ccaacgccag ttatcgtctg gtttaattcg catctgcctc
    37021 tcccctaccc tataaaaccg cagcaacaca atccattgtc gcttcgctta ataacctcaa
    37081 caataaaagt acaaaatgtc taaataagca acagagatga atataaaaca accagggctg
    37141 aaaataaagc cctgattatc ttataaagag gaattcgaag aaataattag atatgggtaa
    37201 cgatttcttt aatcagcggc ggacctttaa aaataaaacc agaataaatt tgcaccagtg
    37261 aggcacccgc agcaatcttt tcacgcgcag cgataaccga gtcgatgccg ccaacaccga
    37321 tgatcggtaa gcgaccgttt aattccagtg acaagcggcg aataatttcg gtgcttttta
    37381 actgaagcgg acgaccactt aagccaccgg tttgatcgca atttttcatt ccctgaacaa
    37441 gagaacgatc gagtgtggta ttggttgcaa taacgccatc aatattatgg cgaactaaac
    37501 tatcggcaac ctggatcaat tcttcttcag aaagatccgg cgcgatcttc actgcgatcg
    37561 gcacatattt atggtgcatc gcttgcaaat cattttgctt atttttaatc gcggttaaga
    37621 gatcatccag cgcttcacca tattgcagcg tgcgtaatcc tggggtattc ggcgatgaaa
    37681 tattgatggc gatatatccc gcataggcat agattttttc catacaaatc agatagtcat
    37741 ctttgccctg ctccactggc gtatctttat ttttgccgat gttaataccc aggacgccgt
    37801 cataatgggc cttttttacg ttctctacga ggttatcaac gccaagatta ttaaagccca
    37861 tacggttgat caaaccttcg gcatctacca gacgaaagag acgcggcttg tcattacctg
    37921 gctgtggacg tggcgtgacg gtaccgatct cgatcgatcc aaatcccatc gcgcctaacg
    37981 cgtcaatgca ctccccgtct ttatcaagac cggctgccag accaagcgga tttttaaacg
    38041 tcaggcccat gcagttaaca ggtttcgcag gcactttctg ccgcaccagt gcttcaaacg
    38101 gcgttcctgt aatacggcgt aattgctgaa aagtaaactc atgagcgcgc tctggatcga
    38161 gctggaaaag ggctttacga acgaaggggt agtacatgaa ctctcctgga ttcccggtgt
    38221 gcaaaccggg ggcgtattat gtgcgagttc ggaccaaaag ggaattgacc tgcggcaaaa
    38281 aaaacgcaaa cgttttccga acctgtttca gggcagattt tgctccttct ttgcactcaa
    38341 cccgccgtaa tcatttatcg cccgccaggc gacatgacac ggtgcgattg cctgacacca
    38401 gcttgtctga cgctggcttt taggcggcac aactcccgca ttacttaacg ctttaccatt
    38461 tacgctcacc tgaatcaggt taatgtagta aggcgttggg ttagtgagtt gtacaccctg
    38521 cgaattccgt gtccagcgta attgttgata agcttccagc gggtcgcccg gcaaaccttc
    38581 gggtcgccag aacagtttaa atatcgagcg catcgccact tttacgctct gattttcaac
    38641 tttgccggat ggcacgctgg caatgcttaa ctcaaatagc gtttcgcgat ccacaggcaa
    38701 gatgtcgctt tccgttctca gcaagcgcaa cgtgcctgta gtaccgggct tcaggagaat
    38761 aagtggcgga gcggccagta atggtgctgg cactgtcgac gcttcccccc cggcccaacg
    38821 cgttgggcgg ttgattttac tgttaataag ccaggattcc tgactggtat tagtcagtaa
    38881 aatagatatc gattctctgt ctgccggaaa gataaatcgc gttccgccga ccaccactcc
    38941 tgcccgcaat tcacccgcca ccagcagcga taaaacggcg aaaatcaaga gcgccaatct
    39001 gttccacgta ttagtcataa tgaagtacaa atgtcgcgct ggcattcact ttacccgact
    39061 gcacatcgct attgacaggt cgcaattgcg cataaaaagt taatggcacc ttaccgctat
    39121 gtaggctgta gtctttactc gcctggttaa gcggaatcaa tatcttcgca tcatccagaa
    39181 tcgcaattgc cagcccgctg gctgcatccg taccactttc aatggacagc atttcgctct
    39241 gcactccgtc gggggttcct gaaaaggtga cgtcaaccgt gctgacatct gttccgcaat
    39301 cctgcaaatt aatgatgaaa gatttctccg ggccataatt cccatgaaaa aaacgtgtca
    39361 gcgagacatc gcctaactgc acattgattt gcttgctatc ctgtgccagc acgcatgtat
    39421 tacggcgaaa cgatccgcta atgttgaccg tgccatcatg ggcaaatagc tcacagcccc
    39481 ccgttaatcc cagtaagaac agaatccaga ttatccgttt caacatcact ccttcactgg
    39541 taaaccagat caaaatagag aaccgccgtc gcattaccgc ccgttgctcc cgcctttgtg
    39601 gacttataac gaagttgata tttgagtgtg ttatccccgg cttttaaggg cgtaagaggc
    39661 tggacctgat tgagcgggat ttcttgctga ctttgcgcat caagaatttg cacggcaatc
    39721 cccgttgcca taccaccgct tccgcctgtg tcagttagtt tgagcagtgt cggcgcaagg
    39781 gtgtcagcct cgccgctaaa aaggacgtcc gcgccagtaa taccagcggc acaaccggtg
    39841 atatcgatat tcaaatcagc agcagtgctt acggtattcg cagcctgaaa atcactgata
    39901 ttaaaatcac caatatgaat attttgtaat gcacttttgg tggcgacatc acagccctga
    39961 tcgaccacat tcccataaat atgcatacta acttgttcag gcccagccat cccgacatgg
    40021 atgttaagaa tgctaaagaa caagataacg cactgaaata tcgttttttt cttcatacgt
    40081 tcacgcgtta atcaaaattt attctcaccg ttgccgtggc ttcaaatcgc ccggtttctg
    40141 gtggtacacc tgtcgtgctg actggataga cattcattcg cgcgatactg tctgcatcat
    40201 caggaataga gagcggtaat gttccaccag caggggaaat aatgttgttc tggctatcgg
    40261 tcaccaccac gcccacgcca ggacgtgacg tcttaatcgc ctggggatag ctggggtctg
    40321 tggttgccgt cagactcagg ttgacgttaa ctaaccccgc gtttcccgtg ttacacctga
    40381 ctggaattgc aagttctgcc gttctggccc caagaggctt atgccctaca acacgaaagt
    40441 tagccgcaaa tacgtcgccc agatcgacct cgattgtttc tccggcattg acggaacatg
    40501 agccaagtga attgatcgta ccagacaggt aatacactaa aaccggtgta cctgtagtcg
    40561 tgcaggaatc acctgcgggt atgttgtagc attcatataa tcgcgccagc gccataggag
    40621 gaatgacgac cgagccaacg aacggctgca atattttcag ggaaaagcta ccgctattac
    40681 cgcttgcccc ctgcccacct aaacgttgac cgccagggtc gcaaatatgc catcgggtat
    40741 ccccccctgt gcccacgttt tcgaaaggaa ccgcagtagg agaactgcct ttaacctgca
    40801 ataaaacttt ggcggcaaga tagtcattaa ttttgtacca gttgtcgcct aaatagacta
    40861 acggtgaatc cgcggcgaat gcccatcggc cacttctgac attgtcttta tcgcaatcac
    40921 aggaaacata ataatcattc gattcgttcc actgataaaa tttgtcttta actacccctg
    40981 ccgtatttgc ttcttcctcc agggttttaa cgccaaaatt gacgccaata tacggattcc
    41041 cctgagtagt atgacactgc ccgctataag cagcggtttg agcgcaccat agcattaaga
    41101 cggcggcaaa aaatagtcgt agtaatgaca cgcatttttc tccaaagatt atctgcatat
    41161 ggcatccatt cgcgtaatcg cagagttaat ttgttgttcg gtaagctcgt aattgatatg
    41221 acaaatttga tcgggagctt cgccccagcg cacgcttatt gttcccttcg ccggtaaacc
    41281 cgccatatag agcgagccat catcatcaat tatgccgcta ttagcctgta cgccgtccag
    41341 cgtcgctatc gcgccaaaac gcagcggtat accctgcttt gatgttttca ttaataccct
    41401 tgcacccgca tgggcattga actccgccag aaccaacgcc cctttggtgg gaaccacgtt
    41461 gactactgca ttttcaagat ccacgttacg ttttaacgac gccgcatcaa gtgcgattcg
    41521 attttcccga taagaagtgg cataaggaac aacggtatat ccgcgccagt cagttttcag
    41581 cccggtgctg ttcgccacac gagtattctc tgcgcctggt gctgcaatca gcacgttagt
    41641 ttcccccagc gattgcccca gggtaatgcc ctgtgaatgg gcaactaaac tgcctgaaag
    41701 agcgtagttc agttgttgtt gactgccgtt atcgctgtag ttgtagccca ctcgggcatc
    41761 tgcaaacgcc cctttgtagt ccatgctggc gctaccatta gccgttttcc cctcgctgtt
    41821 atatccctgt tgcacgctgt agctcaggtt accgtcatcg agtagtgtct ccgttaaacc
    41881 caggttttgt gtaatgtgcc cttcgttatc gatactggtg ttagaggtca tccaggcgta
    41941 attttttttc ctggtatagc tatcattact tctggggagc aacagattga gcggcaggga
    42001 aaaatttagt gcaaacacct gatcagcatc aggttgacca cgggacttac tgtagttcca
    42061 ggaaaccccc agcgagagat ctttaatttg cgtgttgtag ccaaactgta atagccgatc
    42121 ctgttgatcg gtatgccagt aagtttgctg gctaccactt aaataaaacg aaccgtactc
    42181 gcctaattgc tgggagatat tgacctgcag tttgccacgt ttggtgtaaa acaaattgta
    42241 ataacgcgac cacatcggcg tatcttcatc atcaccgtca ttaaattcgt agccatccat
    42301 atgtttatac atggtgtcgg aaagggtgta gaaacccgac gtcgaatagc gatagcccat
    42361 caattgaaaa ttagtgccca attggttgag cgttttggca tacagaaaac gatatgactg
    42421 acctttatgt tcgctttggt ctgccagcgt actcttcgcc tgggtcgcat cgaacgaaat
    42481 tgctccgaaa tcgccaaggt taaatcccag accaaacatg gcggcacggt aatattcagc
    42541 atattgtcca ccaccatacc atgtcgttcc ccacggtccg ccccactgca acgtggcctg
    42601 ggcaaatttg ctctcctgct gttcattact attggttctg tatttcgcca gtgtcaccgc
    42661 gtatttgatt cgcccctgac gctggagtaa tggcacgctg gaaaacggta cgctgtagct
    42721 attgacgctg ccgtccgctt ctttgatttc aactaacaaa tcaccgctcg acgacgtgga
    42781 atagagatca ctaatttcaa aagcaccagg cgatacataa gtttggtaaa tggtgtaacc
    42841 attttgctta atcgttatct gggcattgct tttggcgata ccacgcacca caggcgcaaa
    42901 ccctttcagg ctgtctggca gcatgttgtc atctgacgcc agctgcacac cacgaaagct
    42961 gacactatcg aaaaaatcgc ctgcagtata atcatcacct acggtcagtt cgccttttag
    43021 tggaataacc gcccgctgca aatagctgct gagattcttc cattctgcgg tttggccact
    43081 actgcgactc catgttgaat tgttgcgcaa tctccatggg cctaaattaa cgccactgtt
    43141 taaattcaga aaatagctgt cgccagaatc actgtctgcg ctgctatgaa tactgttagc
    43201 cccgctaaac gaatatccca gtaatagcgc gttgatccct tcatcccaac gttcaggggg
    43261 gacataacca cgggcattac ttttgatggc gatttgcggt actgaaattt ccagacggag
    43321 tttattcaca ttaaacgtta cgctggcatc cggaataatc tccgccaatg gcacacacgc
    43381 ttgtttgtcc agttgtttaa aagccggaaa cgcatcagta ttaacaccca ttgcatccag
    43441 gctttcagtc gtcaaacagg cggcaatccc cttctctggc gttatctcaa caaaattgac
    43501 gttgcgcgta tctacaattg tctgattcag aacaatatcg acacgataaa cccctggcgt
    43561 tattttttgt cctttttcga aacgagacaa atcggccacc attgagggat catctttgaa
    43621 aaaagcagga tcgaaccagg tttcagcttg tgcattgaac gctgccgaca caaacaccac
    43681 cagcccacca ataaacgatg gcactccacc agagcttaac aggctgtgtt gtcgatgagt
    43741 tctatacatc tcgctctctt aactgaagac ggcttacgat ttacagttga gttgcggcgt
    43801 ttccgccccg tagtcattga tggttcgata cgttacgctg ctatttttgg ccccctttgg
    43861 cagagaaaat tggtatttat caaaggggga aatcatttga tttttaacta cttttccacc
    43921 tattttcagg tctgtcaggg taatgaaata aggtgttggg ttactgaccg taaccatgtc
    43981 agctgacgaa gtgcaacgca gtttcgccgg agcctcgcct aagggatacg ctaaaccttt
    44041 tggtcgccag aaaagtttga tgacgctttg tgtggcaatc ttcagggtat ttccctcttt
    44101 ccgcttcgtt gttgaaggga ctgcccgtac gctagtgaag aaaagggttt ctctgtcttt
    44161 cgccaacggc gcaccaatgt acataatgcg taacagattc tcattagccg gattcagcac
    44221 aaatagcggt ggtgtaataa taaaatctgc tgatttttta ttatctgggg tcatcaccca
    44281 tgactgaata agataaggaa catccggact ggtgttacgt acacccagtg aaatttgctc
    44341 ttcaccttct gaataaacaa gacgtgttgc tgcaagtcca atcccgcctt tacctgccgc
    44401 ccatgcggat gagcagctga gtaatattgc cgttaaactt acggtcacaa ttccctttgt
    44461 tatgcaagtt ttcataaata gtcactctta aaaaatatga atatcccctc cagaacactg
    44521 gaggggtact aattaataaa acaattaatg agttctatta ataatagtta atatggaaat
    44581 ttgttaccgc attcacatta cctgaagtcg ctttccccgt agcgatataa tcaactttaa
    44641 aagtcaacgt ctgatctcca tcctgcaaca aaatgttccc ggtttctgtg tctggcgtga
    44701 tggccgttgt accatcattc atatacattt gcagggccac gtttgtagca gcatcagatg
    44761 aggcctggtt agcaaacgct gtaatctgag tagtatccgc agtgccatta aaggtgaacg
    44821 ttgcattttt tgttacggta gtatcacaat cttttaattc aattggcaca tcagtttgca
    44881 cgccagaaac ctgaccattg gtttgcaact tcgtggcact aacatctggc aaagtcacca
    44941 cggaatcttt cgtcgcagct accagggtac aagctggagc aataacttta ccattaaatg
    45001 ttactgagcc atccgtgata gcattatcat cagcagccat aacgctggac gttacacata
    45061 ccacagttat aaacgccgtc aatacacttt ttttcatcgt tatcatcctg atctcttcgt
    45121 aaatatgata atgcctttaa atgacattag acttaataat aacaataatt gtccgccatt
    45181 taaatcaagc atactaaatc caataagtga caaatcttta atttatagat ttcatgaata
    45241 tttaaatatt aactgaacga gtaattcatt gattcaacat ctaatgacta tccaataaat
    45301 aaatatgcgt gatatataaa gccctttctt tagtttattt tcagaaaaag atacacaaaa
    45361 tttatatttg gcattttctt gtctctccga cagactgacc gaattgttat caatgttaac
    45421 aaaaaaagaa caattggtta taaggagaga gtatgcgtgt catcatcctg gcgggtagtc
    45481 ctcgctttcc ttctcgctcc agctccttgc tggaatatgc gcgggaaaaa ctaaattgcc
    45541 tggatgtaga ggtttatcac tggaatctgc aaaacttcgc cccggaagat ctgctttatg
    45601 ctcgtttcga tagtccggca ctcaagacct tcaccgaaca gctgcaacag gctgatggac
    45661 tgatagtcgc tacacctgtg tataaagccg cctattccgg cgcgctgaaa accctgctcg
    45721 acctgctgcc agagcgcgct ttgcaaggca aagtggtgct accgctggcg acgggcggta
    45781 ccgtggccca tctgctggcg gtcgattatg cccttaaacc agttttaagc gcactgaaag
    45841 ctcaggagat cctgcacggc gtgtttgccg atgactcaca agtaattgat taccatcaca
    45901 gaccccagtt cacgccaaat ctgcaaaccc gtcttgatac cgcgctagaa actttctggc
    45961 aggcattgca ccgccgcgat gttcaggttc ctgaccttct gtctctgcga ggtaatgccc
    46021 atgcgtaaca tcattaaact ggcgctggcg ggattgctta gcgtctctac ttttgcggtt
    46081 gctgcagaat cctcgcctga agcgttacgt ataggctatc agaaaggcag tattggtatg
    46141 gtgctggcaa aaagccacca gttactggaa aaacgctatc cggaatcaaa aatctcctgg
    46201 gtggagttcc ccgcgggtcc gcaaatgttg gaagcgttaa acgttggcag tattgatctc
    46261 ggcagtaccg gggatattcc gccaatcttt gcccaggctg ccggggctga tttggtgtac
    46321 gtgggcgtcg agccaccgaa gcccaaagcc gaagtgattc tggtggcaga aaacagcccg
    46381 atcaaaaccg tagccgatct taaaggtcac aaagttgcct ttcagaaagg ttccagttca
    46441 cacaaccttt tactgcgtgc actgcgtcag gccggactta aattcaccga tatccagccc
    46501 acttacctga cgcctgccga tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg
    46561 gctatctggg atccctacta ctccgctgcg ttattacagg gcggcgtgcg ggtgctgaaa
    46621 gacggcaccg atctcaatca aactggatcg ttttatctgg cagctcgtcc ctatgcagaa
    46681 aaaaacggcg cttttattca gggcgtactg gcaaccttta gtgaggccga tgcgttaacc
    46741 cgcagccagc gcgagcaaag catcgcttta ctggcaaaaa cgatgggctt accggcaccg
    46801 gtgattgcct cttacttaga tcatcgccct cctaccacca tcaaaccggt taacgccgag
    46861 gttgccgcct tacagcagca aacggcagat ctgttttatg aaaaccgtct ggtgccgaaa
    46921 aaagtcgata ttcgccagcg catctggcag cccactcaac tggaaggaaa acaattatga
    46981 gtctgaatat gttctggttt ttaccgaccc acggtgacgg gcactatctg ggaacggaag
    47041 aaggttcacg cccggttgat cacggttatc tgcaacaaat tgcgcaagcg gcggatcgtc
    47101 tgggctatac cggtgtgctg atcccaacgg ggcgatcgtg tgaagatgcg tggctggttg
    47161 cagcgtcgat gatcccggtg acgcagcggc tgaagtttct tgtcgccctg cgtcccagcg
    47221 taacctcacc taccgttgcc gctcgccagg ccgccacgct tgaccgtctc tcaaatggtc
    47281 gtgcgttgtt taatctggtc acaggcagcg atccacaaga gctggcaggc gacggcgtgt
    47341 tccttgatca tagcgagcgc tacgaagcct cggcggaatt tacccaggtc tggcggcgct
    47401 tattgctggg ggaaagcgtc gatttcaacg gtaaacatat tcatgtgcgg ggagccaaac
    47461 tgctcttccc gccgattcaa cagccgtatc ctccgcttta ctttggcgga tcgtcagacg
    47521 tcgcccaaga gctggcggca gaacaagttg atctctacct cacctggggt gaaccgccgg
    47581 aattggttaa agagaaaatc gaacaagtgc gggcgaaagc ggctgcgcat ggacgcaaaa
    47641 ttcgtttcgg tattcgtctg catgtgattg ttcgcgaaac caacgatgaa gcgtggcagg
    47701 ccgccgagcg gttaatctcg catcttgatg atgaaactat cgccaaagca caggccgcat
    47761 tcgcccggac ggattccgta ggacaacagc gaatggcggc gttacataac ggcaagcgcg
    47821 acaatctgga gatcagcccc aatttatggg cgggcgttgg cttagtgcgc ggcggtgccg
    47881 gaacggcgct ggtgggcgat ggtcctacgg tcgccgcgcg aatcaacgaa tacgccgcgc
    47941 ttggcatcga cagctttgtg ctttcgggct acccgcatct ggaagaagcg tatcgggttg
    48001 gcgagttgct gttcccgcat ctggatgtcg ccattccgga aataccccag ccgcagcagc
    48061 tgaatccgca aggcgaagcg gtggcaaatg attttatccc ccgtaaagtc gcgcaaagct
    48121 aaggaacacg atgatggcaa cgccagtgaa gaagtggtta ttgcgcgttg ccccctggtt
    48181 tttaccggtg ggcatcgtag cagtgtggca actggcctcc tcggttggct ggctgtcgac
    48241 gcgtattttg ccttcaccgg aaggagtggt gacggcgttc tggacgctct ccgccagcgg
    48301 cgaattgtgg cagcatctgg cgatcagttc ctggcgggcg ctgattggct tttcaattgg
    48361 cggatcgctg ggattaattc tggggctgat tagcgggctg tcacgctggg gtgagcgact
    48421 gctggatacc tcaattcaga tgttgcgcaa cgtgccgcat ctggcgctga tcccgctggt
    48481 gattttgtgg tttggcattg atgaatccgc aaaaatcttt ctggtggcgc tcggtacgct
    48541 attccccatt tatatcaaca cctggcatgg catccgtaat atcgatcgcg ggttggtgga
    48601 aatggcgcgt agctatggat tatccggcat accgctgttt atccatgtga tcctgcctgg
    48661 tgccctgccc tcaattatgg tcggagtgcg ttttgcgttg ggcctgatgt ggctgacgct
    48721 gattgttgcc gaaaccattt ctgccaattc cggcattggt tatctggcga tgaatgcgcg
    48781 ggagtttttg caaacggacg tggtggtagt cgctattatt ctttacgccc tgctcggcaa
    48841 actggctgac gtcagcgcgc agctgttaga gcgcctctgg ttgcgctgga acccggctta
    48901 tcatttgaag gaggccacgg tatgaatact gctcgtctga accagggcac accattgttg
    48961 ctcaatgcag taagcaaaca ttacgcggaa aatatcgtcc tgaaccaact ggatttacat
    49021 attccggcag gtcagtttgt ggcggtggtg ggccgcagcg gtggtggcaa aagtaccctg
    49081 ctgcgtctgc tggcaggtct ggaaacgcca accgcaggcg atgtgttagc gggcaccaca
    49141 ccgctggctg agattcagga agacacgcga atgatgtttc aggatgcgcg tctgctaccg
    49201 tggaaatcgg tgattgataa cgttgggtta ggccttaaag gtcagtggcg cgatgccgcg
    49261 cgtcgggcgt tggctgcggt aggactggag aatcgcgccg gggaatggcc tgccgcactt
    49321 tctggcgggc agaaacagcg agtggcgctg gcaagagcgt tgattcatcg accgggttta
    49381 ttgttgcttg atgaaccgct cggtgcgctg gatgccttaa cgcgactcga gatgcaggat
    49441 ttgattgtgt cactttggca ggagcacggc tttaccgtac tgttggtgac gcatgatgtg
    49501 agcgaagcgg tagcaatggc tgaccgggtg ttgttaattg aagagggaaa aattggtctg
    49561 gatttgacgg tggatatccc ccgtccgcgc cggttagggt cggtgcgatt agcggagctg
    49621 gaagcggaag tgttgcaacg ggtaatgcag cgtggtgagt ctgaaactcg cctgagaaaa
    49681 caggggtaaa atccccgaat ggcggcgcta ttgccgccat tcggttattt attaagccag
    49741 tgctttggtg atcttctcgt acagatcgcc agagagattt tccagccctt tcaactgttc
    49801 cagcgccgcg cgcattttct cctgacgttt ggcatcataa cgtttcaggc gaatcagcgg
    49861 ctcaatcagg cgcgaagcca cctgcgggtt acggctgttg aggtcggtaa gcatttccac
    49921 caggaactgg taaccgctgc catcttcggc atggaacgct gccgggttgc tgcccgcaaa
    49981 cgcaccaatc aacgaacgaa tacggttcgg gttgctcatg gtaaatgagc gatgctgcaa
    50041 caggccgcgc accgtctcca gcacattcgc cgccgggctg gtggcttgca ggataaacca
    50101 tttatccatc accagaccgt cctgatgcca cttgtcgtcg tactcctgca tcagcgcgtc
    50161 acggcatggc agctgtgcag caaccgccgc agaaagcgct gccagcgcat cggtcatatt
    50221 gtttgcttca tggtattgct tgctcaccag cacgtcagcc agatgcgttt caccaaaagc
    50281 aaggaagcgc aggcaggcat tacgcagagt gcgttttgca atatcttcat gctcaacgcg
    50341 gtactcgctc tggtaattcg cgttgtaaat agccagcagc tcatccgcca gttcagtcgc
    50401 cagagtacga gtgagtgctt cgcgtacttc ggcaatagca atcgggtcga tgatatcgaa
    50461 cagttcagcc atttcattga cggatggcag agtcagaatt tctgccgcca gtgccgggtc
    50521 aatcttctca tcgagcagca ccgcgcggaa ggcgtcggcc acatgcaccg gcagagacag
    50581 cggctgccct tgctgatgac gggcgacgtt cagcttgatg taggttgcca gcaaactttg
    50641 cgccgcatcc caacgggaga aatcattacg cgcatgacgc atcaggaagg tcagttgctg
    50701 atcgctccac ttatattcca gtttcactgg cgcagagaat tcgcacagca gcgcaggcac
    50761 cggctggaag tagacattat caaagacaaa ggtctgttcc gcctgagtga cgttcagcac
    50821 ggaattcacc ggatgaccgc ctttctgcaa cgggatcact ttgccttcgt tatcatacag
    50881 ttcgatggca aacggaatat gcagcggctg tttttctgcc tgatccggcg tggctggcgt
    50941 gcgctggctg atggtcaggg tgtactgctc ggtttccgga ttgtagtcgt ctttgacggt
    51001 cacaaccggc gtaccggact ggctgtacca acggcggaaa tgggagagat cgacattcga
    51061 cgcatcttcc atcgcctgca caaaatcgtc acaggtcgct gcgctaccat catgacgctc
    51121 gaaatagagt tgcatccctt tctggaaatt ttcttcgcca agcagggtgt ggatcatgcg
    51181 aatcacttcc gcgcccttct cataaacggt cagggtgtag aagttgttca tctcaatgac
    51241 catatccggg cggattgggt gcgccatcgg gctggcgtct tccgcaaact gcaatccgcg
    51301 catggtgcgc acattattga tacggtttac cgcgcgggaa ccaagatcag agctgaattc
    51361 ctgatcgcgg aagacggtta aaccttcttt caggctgagc tggaaccagt cgcggcaggt
    51421 cactcggtta ccggtccagt tatggaaata ttcatggccg ataacgcgtt caatatcgag
    51481 gtaatctttg tcggtggcgg tgtcggtgcg ggccagcaca tatttggagt taaagatatt
    51541 cagaccctta ttctccattg cgcccatatt gaagaaatcc accgcgacga tcatatagat
    51601 gtcgaggtca tactccaggc caaagcgttc ttcatcccat ttcatggagt ttttcagcga
    51661 ggtcatcgcc cacggcgcgc gatcaaggtt gccgcgatcg acgtacagct ccagtgctac
    51721 ttcgcgacca gaacgcgtgg taaaggtatc gcgcagtaca tcaaagtcgc ctgccaccag
    51781 cgcaaacagg tagcacggtt tcgggaacgg gtcctgccac tgtacccaat ggcgtccgtt
    51841 ttccagttcg ccttgcgcaa cgcggttacc attggaaagc aggaagggat atttgatttt
    51901 atcggcaata attttggtgg taaaacgcgc cagcacgtcc gggcggtcga gataatacgt
    51961 aatatggcgg aaaccttcgg cttcacactg ggtgcaaagc gcatcgcctg actgataaag
    52021 cccttccagc gcggtattcg ccgccgggct tatttcatta atgatcttaa gcgtaaaacg
    52081 ctccggcaaa ttactgataa ccagtgcgcc ctcttcttct ttccaggcgg tccacggctc
    52141 atcattaata tgaacagaaa ccagtttgag gtcttcgccg ttgagacgaa ggggagcatc
    52201 tgatgcacca tgacggacag cctggctgac cgcggtaacg accgtctttt gcgcgtcgag
    52261 gtcaaaggtc aagtcaatat cagtaatctg gtaatccggc gcacgataat cgtgacggta
    52321 tttggcttgt ggctgttgag tcataaataa cctttagcat cttttataga gtctggtgtt
    52381 cagtctattc ctgttgcgta aatcgcgcta tgcagaatct tcatcttttc aggtacaaac
    52441 gcctttattg ctacattttt ataacataca ccgcgtaatg ccatcgacca gaaaggtggc
    52501 atatggtgtg atcggggttc aataaattgc gaaacaaggt atactccagc agttcctgaa
    52561 gatgtttatt gtactaaacg ctcctgtacg aggacgctac tgcgcaccta tgacacaatt
    52621 cgcttctcct gttctgcact cgttgctgga tacagatgct tataagttgc atatgcagca
    52681 agccgtgttt catcactatt acgatgtgca tgtcgcggcg gagtttcgtt gccgaggtga
    52741 cgatctgctg ggtatttatg ccgatgctat tcgtgaacag attcaggcga tgcagcacct
    52801 gcgcctgcag gatgatgaat atcagtggct ctctgccctg cctttcttta aggccgacta
    52861 tcttaactgg ttacgcgagt tccgctttaa cccggaacaa gtcaccgtgt ccaacgataa
    52921 tggcaagctg gatattcgtt taagcggtcc gtggcgtgaa gtcatcctct gggaagttcc
    52981 tttgctggcg gttatcagtg aaatggtaca tcgctatcgc tcaccgcagg ccgacgttgc
    53041 gcaagccctc gacacgctgg aaagcaaatt agtcgacttc tcggcgttaa ccgccggtct
    53101 tgatatgtcg cgcttccatc tgatggattt tggcacccgt cgccgttttt ctcgcgaagt
    53161 acaagaaacc atcgttaagc gtctgcaaca ggaatcctgg tttgtgggca ccagcaacta
    53221 cgatctggcg cgtcggcttt ccctcacgcc gatgggaaca caggcacacg aatggttcca
    53281 ggcacatcag caaatcagcc cggatctagc caacagccag cgagctgcac ttgctgcctg
    53341 gctggaagag tatcccgacc aacttggcat tgcattaacc gactgcatca ctatggatgc
    53401 tttcctgcgt gatttcggtg tcgagttcgc tagtcggtat cagggcctgc gtcatgactc
    53461 tggcgacccg gttgaatggg gtgaaaaagc cattgcacat tatgaaaagc tgggaattga
    53521 tccacagagt aaaacgctgg ttttctctga caatctggat ttacgcaaag cggttgagct
    53581 ataccgccac ttctcttccc gcgtgcaatt aagttttggt attgggactc gcctgacctg
    53641 cgatatcccc caggtaaaac ccctgaatat tgtgattaag ttggtagagt gtaacggtaa
    53701 accggtggcg aaactttctg acagccctgg caaaactatc tgccacgata aagcgtttgt
    53761 tcgggcgctg cgcaaagcgt tcgaccttcc gcatattaaa aaagccagtt aatatcatca
    53821 gggagctaat cggctccctt tttttacctt taattccgaa atctttcgct gcatttgcga
    53881 attctgcttg tctgattgca gatgccaggt aacataggta tccccccatt tacgggtgga
    53941 caagtgttta ttttttccga ctattaacag agagaatatt atgagcgttg tgcctgtagc
    54001 cgacgtactc cagggccgcg tagccgttga cagcgaagtc accgtacgcg gatgggtacg
    54061 tacccgccga gattcaaaag ctggcatctc cttcctcgcc gtttatgacg gttcctgctt
    54121 tgatcctgta caggctgtca tcaataattc tctgcccaat tacaatgaag acgtcctgcg
    54181 tctgaccacc ggttgctcgg tcattgtgac gggtaaagtc gtggcgtcgc cgggccaggg
    54241 gcaacaattt gaaattcagg ccagcaaggt tgaagttgct ggttgggttg aagatccaga
    54301 cacttacccg atggcggcaa aacgccacag cattgagtat ctgcgtgaag tcgctcacct
    54361 gcgtccgcgc acaaacctga ttggtgccgt cgcgcgcgtg cgccatacgc tggcgcaggc
    54421 gctgcatcgc ttctttaacg agcagggatt cttctgggtt tcaacgccac tgattactgc
    54481 gtctgatacc gaaggggctg gcgaaatgtt ccgcgtttct acgctggatc tggaaaatct
    54541 gccgcgtaac gatcagggca aagtggattt cgacaaagac ttctttggta aagagtcttt
    54601 cctgaccgta tctggccagt tgaacggcga aacctacgct tgcgcattat ccaaaattta
    54661 taccttcggc ccgactttcc gtgctgaaaa ctccaacacc agccgtcacc tggcggagtt
    54721 ctggatgctg gagccggaag tggcgtttgc taacctgaac gatatcgcgg gtctggctga
    54781 agccatgctg aaatatgtct tcaaagcggt tctcgaagaa cgcgcagacg acatgaaatt
    54841 cttcgctgaa cgcgtagata aagatgccgt ttcacgtctg gaacgcttca ttgaggccga
    54901 tttcgcgcag gtggattaca ccgacgcagt gaccattctc gaaaactgcg gcaggaagtt
    54961 tgaaaacccg gtttactggg gcgtcgatct ctcttctgag catgagcgtt atctggcgga
    55021 agaacacttt aaagcaccgg tagtggttaa aaactatccg aaagatatta aagcgttcta
    55081 tatgcgcctc aacgaagacg gtaaaaccgt tgcggctatg gacgttctgg ctccgggcat
    55141 cggtgagatc attggtggct cccagcgtga agaacgtctg gacgttctgg acgagcgtat
    55201 gctggaaatg ggcctgaata aagaagatta ctggtggtat cgcgatctgc gtcgctacgg
    55261 tactgttccg cattcaggtt tcggtcttgg ttttgaacgc ctgattgctt acgtaactgg
    55321 cgtgcaaaac gtgcgtgatg tgattccgtt cccacgtact ccgcgcaacg ccagcttctg
    55381 attttcttcc tcaaagagcc agcgcctgct ggctcttttt tatcctttct gtcaagttat
    55441 ctgtttgtta agtcaagcaa tctatttgca accccgccat aacgatcatc ctgttacgga
    55501 atattacatt gcaacattta cgcacaaaaa ataacctgga ttcttattgc ggattagttt
    55561 tttcttagct gatagcacaa ttttcatact attttttggc attctggatg tctgaacgag
    55621 gatttagtgc caggtcgata aagtttccat cagaaacaaa atttccgttt agttaattta
    55681 aatataagga aatcatataa atagataaaa attgctgtaa atatcatcac gcttctatgg
    55741 aaatatgacg gtgttcacaa agttccttaa attttacttt tggttaca
//
LOCUS       DAOFQD010000015        55751 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.015, whole genome shotgun sequence.
ACCESSION   DAOFQD010000015 DAOFQD010000000
VERSION     DAOFQD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.015"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            108..725
                     /gene="tdk"
                     /locus_tag="QBF46_000941"
     CDS             108..725
                     /gene="tdk"
                     /locus_tag="QBF46_000941"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDQ6673162.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLMAIQERHRHD"
     gene            complement(1027..3702)
                     /gene="adhE"
                     /locus_tag="QBF46_000942"
                     /gene_synonym="adhC"
     CDS             complement(1027..3702)
                     /gene="adhE"
                     /locus_tag="QBF46_000942"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDQ6673163.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4179..4826
                     /gene="ychE"
                     /locus_tag="QBF46_000943"
     CDS             4179..4826
                     /gene="ychE"
                     /locus_tag="QBF46_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDQ6673164.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(4984..5145)
                     /locus_tag="QBF46_000944"
     CDS             complement(4984..5145)
                     /locus_tag="QBF46_000944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673165.1"
                     /translation="MSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQA
                     QDYDVAKIT"
     gene            5564..7195
                     /gene="oppA"
                     /locus_tag="QBF46_000945"
     CDS             5564..7195
                     /gene="oppA"
                     /locus_tag="QBF46_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDQ6673166.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            7281..8201
                     /gene="oppB"
                     /locus_tag="QBF46_000946"
     CDS             7281..8201
                     /gene="oppB"
                     /locus_tag="QBF46_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDQ6673167.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            8216..9124
                     /gene="oppC"
                     /locus_tag="QBF46_000947"
     CDS             8216..9124
                     /gene="oppC"
                     /locus_tag="QBF46_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDQ6673168.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            9136..10149
                     /gene="oppD"
                     /locus_tag="QBF46_000948"
     CDS             9136..10149
                     /gene="oppD"
                     /locus_tag="QBF46_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDQ6673169.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            10146..11150
                     /gene="oppF"
                     /locus_tag="QBF46_000949"
     CDS             10146..11150
                     /gene="oppF"
                     /locus_tag="QBF46_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDQ6673170.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(11203..11532)
                     /locus_tag="QBF46_000950"
     CDS             complement(11203..11532)
                     /locus_tag="QBF46_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDQ6673171.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(11567..13027)
                     /gene="cls"
                     /locus_tag="QBF46_000951"
     CDS             complement(11567..13027)
                     /gene="cls"
                     /locus_tag="QBF46_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDQ6673172.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            13170..13343
                     /locus_tag="QBF46_000952"
     CDS             13170..13343
                     /locus_tag="QBF46_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDQ6673173.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(13398..14651)
                     /gene="kch"
                     /locus_tag="QBF46_000953"
     CDS             complement(13398..14651)
                     /gene="kch"
                     /locus_tag="QBF46_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDQ6673174.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDELETKADSKESAQK"
     gene            15156..16349
                     /locus_tag="QBF46_000954"
     CDS             15156..16349
                     /locus_tag="QBF46_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6673175.1"
                     /translation="MITDTKLRKALGKKRDDIEIISDSHGLNARISQAGKISFFYRYR
                     WAGKAVKLNVGDYPAMSITQARERRQQFRNWLTEGLDPREQVKLDKQTRQEAMSVAEA
                     FNYWIERHCIANGLVKVDYYRQVFEKHIAEPMKNVKVDNTAKMHWINVFDSIESRVMA
                     HYMLSLCKRAFRFCVNRSVIASNPLEGLLPSDVGQKPKKRTRRMDDDDLRKIYQWLKS
                     HMSIESVFLVKFIMLTGCRTAEIRLSERSWFRLDDNEWVVPAGSYKTRVHIRRGLSDA
                     AVNLVRNHLKKINTNHLVTSQRKIDGGIKDSPVHSPVASNYARSIWNGTGMAEWSLHD
                     MRRTIATNLSELGCPPHVIEKLLGHQMVGVMAHYNLHDYIDDQKHWLRVWQSHLEEII
                     GEPFS"
     gene            complement(16585..16824)
                     /locus_tag="QBF46_000955"
     CDS             complement(16585..16824)
                     /locus_tag="QBF46_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4060 family protein"
                     /protein_id="HDQ6673176.1"
                     /translation="MKLINRSKQSPIGRRACDVALAAHLQTYGDYGRSKMKETYTVKV
                     EGVKVWVEVVNRKASYVATAMTGMRRLRSLPGQVG"
     gene            complement(16827..17711)
                     /locus_tag="QBF46_000956"
     CDS             complement(16827..17711)
                     /locus_tag="QBF46_000956"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010989138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDQ6673177.1"
                     /translation="MRYGSVCSGAAAMPIEEPALRSWQRPFLKWAGGKYSLLPELDRL
                     IPAGKRLIEPFVGGGSVFLNSDKHERFLLADVNADLINLYQMLAVVPDSVIYEAMKAF
                     RHLNDAENYTVIREAFNAQRLDAVERAAAFLYLNRHCFNGLIRYNLDGFFNVGFGKYK
                     AQYFPEEEIRAFKRKAHACVFMNAGFRRTLALAGDGDVVYCDPPYEPLPGTAGFTNYA
                     AGGFSWDSQVELTESCVAAHQRGAKVVISNSTAPRVIELYEQHGFTLHRVSARRAISS
                     KGSTRETASDVVATLGVQ"
     gene            complement(17708..17866)
                     /locus_tag="QBF46_000957"
     CDS             complement(17708..17866)
                     /locus_tag="QBF46_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HDQ6673178.1"
                     /translation="MTHAHDDIRVGAVRLPWLKEKNGWLLPWGEVVTNPLKAQRLAEE
                     LNEKQVAA"
     gene            complement(17863..18291)
                     /locus_tag="QBF46_000958"
     CDS             complement(17863..18291)
                     /locus_tag="QBF46_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDQ6673179.1"
                     /translation="MKRTPFYRRPGRTGQFSGLRERVIWMIQTRGRPVTGSEIAEKFG
                     VTLIEFNRVANGITRGSGQIAQIVESEKWLNEDGICDRTFDLVTKPKVVTPQGKSRLF
                     TRRAIEQSQEGRRQECIARAARRSRLIAQGLYIDEMESIL"
     gene            complement(18288..18854)
                     /locus_tag="QBF46_000959"
     CDS             complement(18288..18854)
                     /locus_tag="QBF46_000959"
                     /inference="COORDINATES: protein motif:HMM:NF021128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDQ6673180.1"
                     /translation="MATASNFGLIMANDGKAFGEPAKRYALQLALEQIKGCKSEFGFS
                     NEHMERGHEQEPIARMLYEEMNFVDVDNGGFFDHETYGDSPDGLVGQDGLVEIKSVIA
                     ATHYSTLTRGSFDPAYRWQLVGHLDCSGRDWVDFISYCSDFPDGKQLIVYRLTAAECE
                     TEIARLRARRNDFLELVADTKRRILELE"
     gene            complement(18851..19177)
                     /locus_tag="QBF46_000960"
                     /pseudo
     CDS             complement(18851..19177)
                     /locus_tag="QBF46_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19248..19445)
                     /locus_tag="QBF46_000961"
     CDS             complement(19248..19445)
                     /locus_tag="QBF46_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HDQ6673181.1"
                     /translation="METLFALVLTVAMTNGDYQDVILGVYDSQQECSLAATEQKVSAE
                     CWPVESIIRNGEFPAKSIAQQ"
     gene            complement(19730..20026)
                     /locus_tag="QBF46_000962"
     CDS             complement(19730..20026)
                     /locus_tag="QBF46_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDQ6673182.1"
                     /translation="MIDDIKRIDSMINALRNMKQDIKRQQKLSEINSLDLSPKQAQKR
                     NADADWIAMEQIKRRHELHALSVELGFAERRESYAPFELTDGWHRFDHKPREPQ"
     gene            complement(20603..21355)
                     /locus_tag="QBF46_000963"
     CDS             complement(20603..21355)
                     /locus_tag="QBF46_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDQ6673183.1"
                     /translation="MLNTTQASFSNLHIYHFAKSSNNAGMENKEIRKANLEALYEKRQ
                     HESGMTKAQFAELIETSPAALSQLLGPNPHRNIGDKMARKIETALDLPFGWMDVLHAS
                     EEPSNVAFRGLNETKGSYPVISWVSAGQWMEAVEPYHRRAIDRWYDTTVDCSEDSFWL
                     DVKGDSMTSPAGLSIPEGAAILVDPEVEPRNGKLVVAKLEGDNEATFKKLVIDAGRRF
                     LKPLNPAYPMLEINGNCKIIGVVVDAKILNIP"
     gene            21397..21630
                     /locus_tag="QBF46_000964"
     CDS             21397..21630
                     /locus_tag="QBF46_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6673184.1"
                     /translation="MSTELHRWRKAATTDEWAQLAKLANTTPGYLDQIAYGNRRASPE
                     MASAIEKGTKNFHRQAPVLKESLVFASPRDTAA"
     gene            21648..22193
                     /locus_tag="QBF46_000965"
     CDS             21648..22193
                     /locus_tag="QBF46_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673185.1"
                     /translation="MHSLAYQQGNKFSPTAMIYQNRREPDSAALNIDGIRAAVRAWAA
                     DCRSREFVAALIVEEWRASGGTGLDIPTDSHRQMQKVFRWIDGDTEYAANNIRQLAPA
                     IMSVLPLEYRNRLAQQNDTMSLIASAMKECAEAKQAVLLDAPEHQKLKEVSEGIASLF
                     RLMPEQVGPLMTMVTSMLGVI"
     gene            22279..23187
                     /locus_tag="QBF46_000966"
     CDS             22279..23187
                     /locus_tag="QBF46_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDQ6673186.1"
                     /translation="MSNTAEIINFPNKTEQPGGRMADLSNGYTKVANEIQQLKPRLRL
                     SGREWQCFEAVIWLTYGWNKKQDRVTNTVIAELTGLSDTHVSDALKSLAERKIIFSQK
                     QGMMKIVGVNTDLSAWILDKPETGRKFPKTGKSFPKSGITFPKTVDTQYKNKNSIKRS
                     SSENSDESSDARLKKFLSTHPEAAVYTPSGAKWGSAEDLETAKWISSRVKLINPTCKA
                     PDMTSWSNTVRLMRQIDNRSHQDICALYDWASKHHFWQTNILSPESLRKQWDKLTMQR
                     NAGGEQRAVKPDLDFNNTDWAYGVIR"
     gene            23184..23873
                     /locus_tag="QBF46_000967"
     CDS             23184..23873
                     /locus_tag="QBF46_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016066203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
                     /protein_id="HDQ6673187.1"
                     /translation="MKSLAEQMRNHDREQMSRMAHNLPEQYQECAPVEQVAQVFNKLF
                     NELRAAFPASMANFRTQEDLNEFRRQWLLAFQENGIHTMAQVDAGMRIARRQERPFLP
                     SPGQFVAWCKQSGGALGVNVDQVIAEYWDWRNRSFEFISSEQFPWSQPVMYHICVELR
                     HRSTERQLTHGELAREAGDLLDMWERRVTEGKPVPPVRRAIAAPAAEQGPTPIQLLLA
                     KYNRNKSNGMV"
     gene            23875..24219
                     /locus_tag="QBF46_000968"
     CDS             23875..24219
                     /locus_tag="QBF46_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003859817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDQ6673188.1"
                     /translation="MNITIREQVLAALRNNPGLNNAKLAGLIGMDTKKISGTVSTLLA
                     DGLIEFEGKHGQRLYRLTDYGMKYAPETIPAMPKGNSKLVQRTEANVICQECRNSAAM
                     RRVLMVWGRVGV"
     gene            24216..24611
                     /locus_tag="QBF46_000969"
     CDS             24216..24611
                     /locus_tag="QBF46_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDQ6673189.1"
                     /translation="MSNIDKRALREAANAANAASWGNWEPYKPHKGARGYEVKVGVKA
                     VAQHCLKVDSVFIAAANPVTVLALLASLEAAEKRIAELEAKLDSADKLQDSAFRHGLQ
                     HGFSFGQTDNQAGFEECLSAYGARGKDNG"
     gene            24604..24804
                     /locus_tag="QBF46_000970"
     CDS             24604..24804
                     /locus_tag="QBF46_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673190.1"
                     /translation="MANSLLETCNDWQILRAEILARNPNMAMTLQKLDIMIEHSVRAA
                     IEISHRVDWDFSEAERKAKAVN"
     gene            24804..25421
                     /locus_tag="QBF46_000971"
     CDS             24804..25421
                     /locus_tag="QBF46_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003859822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDQ6673191.1"
                     /translation="MELSRDKIKEIINDDWLLMDDCEGNKNCDVVKEMARIALASLEA
                     EPVAWLLSGGGAKNNVSFDSGNAYADPLREVTPLYTAPPAPVSVPAAMEIDDDFDSAF
                     EHGKAVGWNAYRAAMLQAEPVSNSDELTDGWVACSKRMPSPSTGVIVAAPWQSAPGGY
                     AMKWATNCPGHPDADIDGWIIPGASWSPTHWMPLPAAPQQEDIIC"
     gene            complement(25423..25860)
                     /locus_tag="QBF46_000972"
     CDS             complement(25423..25860)
                     /locus_tag="QBF46_000972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673192.1"
                     /translation="MNNGVFTTVITGVSVFVLGQIFVKSMLDPYISFKEHLGMVSAIL
                     LREQNKILSINAKSEVINEIKQASALLLSKSNAIPLYGMLATLRLLPRYKDVLKASWN
                     LNLIASILEEGRNTTPKETYTTISNSLNAVGSKLGVVVTYRPS"
     gene            26229..26684
                     /locus_tag="QBF46_000973"
     CDS             26229..26684
                     /locus_tag="QBF46_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HDQ6673193.1"
                     /translation="MIISKDGIKLHRGNLGAITQHLKPLLENGECFRLQLKDWREKRS
                     LSQNSLSHVWYKEISDYLIKSGRTDATPAWVKRNLKKTYLGYEEVEYTDFVTGIKTIE
                     LELRHTSDLDTGDMHHFMCQVEGWCAQFGLVLTIPQSSEFQVLRDKQEA"
     gene            26684..26854
                     /locus_tag="QBF46_000974"
     CDS             26684..26854
                     /locus_tag="QBF46_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HDQ6673194.1"
                     /translation="MSTPLSRVITNEIFRVPARRKPKPAVKPSDIPTLKDYTARLVDQ
                     KWLRLAARRKSA"
     gene            26851..27519
                     /locus_tag="QBF46_000975"
     CDS             26851..27519
                     /locus_tag="QBF46_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDQ6673195.1"
                     /translation="MSMYQRINGADWRNIFVVGDLHGCYTLLMNELEKVSFDPARDLL
                     ISVGDLVDRGAENVECLDLITMPWFRAVRGNHEQMMVDGLSEHGNVNHWLVNGGGWFF
                     NLDYDKEVLAKALVHKAAELPLIIELVTSDRKIVICHADYPHNEYAFDKPVPKDMVIW
                     NRERVSDAQGGIVSPIAGADLFIFGHTPARQPLKYANQMYIDTGAVFCGNLTLVQVQG
                     GDHE"
     gene            27512..27796
                     /locus_tag="QBF46_000976"
     CDS             27512..27796
                     /locus_tag="QBF46_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673196.1"
                     /translation="MSKTYRSKKWLAAVGQIEQCVLCGAWGVQVAHRNEGKGIGMKTD
                     DCATAAICVTCHSEIDNGKGLSRDERRQLMDRAIVLTIIQIARRGLVVPA"
     gene            27793..28152
                     /locus_tag="QBF46_000977"
     CDS             27793..28152
                     /locus_tag="QBF46_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDQ6673197.1"
                     /translation="MKIYDITPIGKPRMTRADKWKQRPPVMRYRAFCDEVRLRKLTMP
                     ESGSHVTFVLPMPPSWSKKKRAEFAGKPHQAKPDCDNMLKALMDALYEDDAHIWDCRI
                     TKVWGEKGQIIIGECAP"
     gene            28149..28265
                     /locus_tag="QBF46_000978"
     CDS             28149..28265
                     /locus_tag="QBF46_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673198.1"
                     /translation="MTRDEITRYQAESVKRANLPPIAKHSQTKTNQPQKEAA"
     gene            28262..28951
                     /locus_tag="QBF46_000979"
     CDS             28262..28951
                     /locus_tag="QBF46_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HDQ6673199.1"
                     /translation="MNSQQLEYVRQQLIVATADLSGATKGQLVAFAENAQFTATARSR
                     GRKKVFDKDKQRMVNPDGQPMSGNQSRAKGSSIALVGPVEYGTASWRRAVLSLEDHQK
                     AWLLWNYSENVRWCYQVEITQWAWAEFREQLGAKKVAGKTMERLKKLVWLAAQDVKAE
                     LAGRETYEYQKLAAMVGVTPKNWSETFTERWEEMKTTLRRLDSDSLLQVTRSRSQQKA
                     TNLDSSLAKLD"
     gene            complement(29897..30067)
                     /locus_tag="QBF46_000980"
     CDS             complement(29897..30067)
                     /locus_tag="QBF46_000980"
                     /inference="COORDINATES: protein motif:HMM:NF021415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HDQ6673200.1"
                     /translation="MIIKPCPYCGKLINPESLVCSHCRIVNPFVKASRREKAKNVLVI
                     ALVAAFLIWMIL"
     gene            30275..30553
                     /locus_tag="QBF46_000981"
     CDS             30275..30553
                     /locus_tag="QBF46_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDQ6673201.1"
                     /translation="MTRMSTIYSRLSYGTGTTLTGCGVSAKAYAETAKTAKEVSWMLA
                     DRIAGLSLSDWAIIVGIACTVITCAVNWYFRWKEREDRRNGYATKAEE"
     gene            30531..31097
                     /locus_tag="QBF46_000982"
     CDS             30531..31097
                     /locus_tag="QBF46_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDQ6673202.1"
                     /translation="MPPKLKNSVIAAIPAGAIAIAAALITGPTGNDGLEGVRYQPYRD
                     VVGVWTVCWGHTGKDIILGKTYTKAECQALLDKDLNIVARQINPYINAPIPETMRGAL
                     YSFAYNVGAGNFKTSTLLRKINQGDSKGACEQLRRWIYAGDKQWKGLITRREIEREVC
                     LWAEKPQVLGDGLGPLNPGIPVSVPGVF"
     gene            31097..31633
                     /locus_tag="QBF46_000983"
     CDS             31097..31633
                     /locus_tag="QBF46_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673203.1"
                     /translation="MKPRKITIVAVLLVAVVIIIAVLSVLLVRSRSALETEQSENRVL
                     RNDNALQATVITTQAFNFNRFNQIAENANRLNSLIDAGTEKTVIEYREILRREKTCDL
                     PVPADVAGGLLKYAYRLRASAMHPDTGNTNATDDSTAAASSMTYCQAVLWIEPLLAVI
                     EKGNNNLAGIREIEKLRK"
     gene            32208..33110
                     /locus_tag="QBF46_000984"
     CDS             32208..33110
                     /locus_tag="QBF46_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDQ6673204.1"
                     /translation="MPNWSDVLGEITALAHKSPMDEVRRKYLSQLSNHTGRNVITYYS
                     GWLQHGGAEVRHLTQMTDDDKNGLMTAINGLDVSKGLDLILHTPGGDIAALESIGHYL
                     RSKFGTNIRAIVPMISMSCGTMLACCANEIIMGKQSNIGPIDPQFNGFSTHAIIEEWN
                     RAQTEIFQNPAAVQMWQFILQKLNPTIIGECEKAIKWANEIVKHWLMTGMFDNDPEAE
                     SKATHVCSELNNHHTTYTHSRHIHFDKAQKIGLNVTELESDQVLQDLVLTIHHSYMHS
                     FGGAPLAKIIENHNGNAMIWNIQS"
     gene            33301..33825
                     /locus_tag="QBF46_000985"
     CDS             33301..33825
                     /locus_tag="QBF46_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDQ6673205.1"
                     /translation="MNNPSVIPAFDFREMVQAKNGEVVTTSRKVAKYFGKRHGDVLRK
                     IEQVKSDCSSEFGQRNFASADYIDEQGKVRPMYSLTRDGWIMVVMGFTGRAAAAIKES
                     YISAFNWMSEQLSRRMAMGEEMQHRYAIKETRSKLKGTIGSRLMNERKKEKRVLALEH
                     EHIMQVTQPELLIG"
     gene            33903..34100
                     /locus_tag="QBF46_000986"
     CDS             33903..34100
                     /locus_tag="QBF46_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDQ6673206.1"
                     /translation="MKILVEHNSKVIWMRDNETSEGVACRSYIKDGVQQKIIAALEDA
                     LAQAKGELLCWNDSDAVSDIS"
     gene            complement(34059..34328)
                     /locus_tag="QBF46_000987"
     CDS             complement(34059..34328)
                     /locus_tag="QBF46_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDQ6673207.1"
                     /translation="MQFLHKRMHLNAGDVVVVDCSHQCNILLTTDSNFNSYKNNRGFH
                     HHGGGGFFERLPTRLVAPHMGYWNVTIDLGGGSGNVTHSIRVIPA"
     gene            34506..35144
                     /locus_tag="QBF46_000988"
     CDS             34506..35144
                     /locus_tag="QBF46_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673208.1"
                     /translation="MATLKDLSNQLKQLQKQIPFATAQAMTKVVRQIELAQKTAFERH
                     LESPTPFTVKSVGSVAARKNNLTAKVFVRDTAAGYLEPFEFGGEHKLNSQALLNPKNV
                     KLNKYGNMPRNKLSQLKTKENVFVGEVDGVNAVWQRKKPMKAKKRRAKRSANGTRRPK
                     RKQRSPKLLIRFGDALPVTPVLGYMDRSHTMANALLPSALNQAIAEAIRTAK"
     gene            complement(35155..35658)
                     /locus_tag="QBF46_000989"
     CDS             complement(35155..35658)
                     /locus_tag="QBF46_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673209.1"
                     /translation="MGNNIIKITFVAAVLASPIAHAQWVTNTEDDLFSGGKKAMMLGE
                     VSSDNGAIVFDCTKEKLSAAYVEMDKSTESLSEVPMDLIMKVDGNTAVKLDATLSRRN
                     VQSLQIQSDDADQLKTVLKQLQGAKSKVLVGVQTKDGGNQHSMSANVSGSTTAVNSFI
                     KACEINL"
     gene            35948..36517
                     /locus_tag="QBF46_000990"
     CDS             35948..36517
                     /locus_tag="QBF46_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673210.1"
                     /translation="MTTMNQSQYAQHSGVDRKTIGRWIKAGRFIVMDGDLIDVEASDA
                     ALKKNRDGKDPRASNAKKKKTPVVSDNDDDGDEINKTVRQIMLTEGADLSREEAGRIR
                     ENYMALQAKLQYEKDSGQLIELTAAEEVLFNAFRQQRDAWLNWPSRVAPLMAADLDVP
                     ADRMTEVLIEHVHKHISVLGEPEFNPAED"
     gene            36459..38582
                     /locus_tag="QBF46_000991"
     CDS             36459..38582
                     /locus_tag="QBF46_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDQ6673211.1"
                     /translation="MSTNTSQSSESQSLTRQKIERLELSVRKGWTPPPRISVPQWADD
                     YRKLAKEAGSTSGNWETSTVEIARGPMLAATESGVHIITVMCCTQLMKTALLENLFGY
                     FAHLDPCPILLLQPKEEAAEQFSKERISPLVRVTPVLRKIIGDSKQKSSKETILYKAF
                     TGGFLALAGAGSPDNLARRPIRVLLADEVDKYPITREGDPIALAEERTATFGLTWLSV
                     RACSPTVEDESRIADSYADSDQRRASVVCPHCGHRQFPDFFKHVQWPKEGDKHLTKSA
                     MLYCECCGSGWSEGQRLRALHTIRWHQTRPFECCGERHSPLMDYDLAWRAADEGSVEK
                     VWQWSESERHAVYRAICPSCGKEAVDNHHAGYQASKLFSPWQKDKPSDIAKKYIDAKG
                     DPDKEQAWWNTQMGLPHRPNHGKQLPVDVLLARREIFPAVVPDGVALLTAGVDTQDDR
                     FEITITGWGRDEESWSVAHDVIYGDLETEEPWKRLDAYLKQIWRRGDGRGLNIMATCM
                     DSGGHHTQKVYEFAKERLGRRVWAIKGESAQGGKRNPVWPTKRPSSKSKASFRPVILG
                     VNSAKDVIRGRLHLEPPKPGAAAAGYMHFPDDRDLGYFNQLLAERLVYKVISGQRYSI
                     WEAIPGRANEALDCLVYSYAALCGLKHMGLKLNVRAANLEADPDKFLPAPVGQEEKIN
                     YELPGAVIEEPAPVKRKRISQLLPK"
     gene            38591..38854
                     /locus_tag="QBF46_000992"
     CDS             38591..38854
                     /locus_tag="QBF46_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDQ6673212.1"
                     /translation="MFNRNTSLLAGAMTDDQLRDALAKAQQAYIDLATGSHGVSFSYT
                     QGDGTRSVSYQQSTLADLLALIQLLQAQLGIISRPRKPARFRF"
     gene            38854..40491
                     /locus_tag="QBF46_000993"
     CDS             38854..40491
                     /locus_tag="QBF46_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ6673213.1"
                     /translation="MNKVQILGSDGQPLRQQRPSMLVGGSRVPYDAADSFSDQLANWQ
                     PALWSPDNEINIYRDRIVSRARDLVRNDGWANGAVTRLLDNAVGANFRPIMKPDYRVL
                     RMITGNKAFDASWAEEYGKALDGHWRTWSNDPGRYCDVERKLTVSQMLRLGFRHKLID
                     GDALAILQYRTDRLGPGRGRYATTVQIVDPDRLSNPQQNFDMPNVRGGVEIDADGAPV
                     AYHIREAHIGDWWSGAKTMTWQRIPRETDWGRPHVVHDFDHERGAQHRGNGILTPVIQ
                     RLKMLVKYDQSELEAAILNAIFAAYIESPYDPAMVQSALGETYDESELGTYQDGRVEF
                     HNDRRLTLQNGARMPILYPGEKITTVNAARPYSNFEVFESAVLRNFSSGTGLSPQQVT
                     QDWSDVNYSSARSSLLEAWKTLTRRRDDFSTGFAQPILTAFVEEVHDNEDLPLPAGAP
                     DFVDARAAYSRARWMGPGRGWVDPVAEKKGAILGLDAGLSTLEIEVGENVGEDWEEVL
                     DQRQREIESCLKRGLPLPSWAQADQFASQTITEPEEK"
     gene            40488..41354
                     /locus_tag="QBF46_000994"
     CDS             40488..41354
                     /locus_tag="QBF46_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDQ6673214.1"
                     /translation="MNLPHLAQRLFNTPLALHPSKAEVIMASVMDRFGISKIESSLAM
                     EDDWYGYDDNRGRESRSDPGYDNVLGVAVIPICGTLVQKLGSLRPYSGMTGYDGIRQA
                     FLTAMEDPDISGICLDIDSPGGEVAGCFDLVDVIYGSRGKKPIHAILTESAYSAAYAI
                     ASAADRISVPRTGGVGSVGVITMHLDWTQRIKDDGLKVTIITYGSRKAEGSPLRELSD
                     EALAAIQQDINTMGELFVNTVARNRGISAKVIKSTQAACFMAADGVEIGLADEVCPPD
                     AAFKNLLEKTGA"
     gene            41356..41946
                     /locus_tag="QBF46_000995"
     CDS             41356..41946
                     /locus_tag="QBF46_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673215.1"
                     /translation="MAKKKTFSFAHLIGLGPSASEEEEDKKAKKAKARRAEKDEREDD
                     ADDDERDDDAEEDERDDDAEDDGDDPDASEDDDSEDDGDDDRKESKAVKNARAAERKR
                     CARIFGSKHAAANPSLAASLAFNTGMSSAAAINVLASSAPAAAASQPSRKRSLDQRMQ
                     ESHQVRLNPDSGQKETGKSALVSKMTGLYNSTRGEK"
     gene            41946..42350
                     /locus_tag="QBF46_000996"
     CDS             41946..42350
                     /locus_tag="QBF46_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDQ6673216.1"
                     /translation="MDQFGQNAFAPGMKSALFVPDQLVAGTLQLVTDTGIITGGAFKR
                     GTVLGLVAASGKYTQCVKTAEDGSQLPVAILVDDVDASSSDQNGGLYLMGEFNQHRII
                     FDNSWTTADLKKALRPLAIFLKDSDQAPVTTS"
     gene            42448..43497
                     /locus_tag="QBF46_000997"
     CDS             42448..43497
                     /locus_tag="QBF46_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDQ6673217.1"
                     /translation="MENIFDTSVLVQVVPNLKTSQNWLLDRFFPNVVTYETEEVAIDV
                     DVGLRRMAPFVSPLVEGKLVESRKYQTNTFKPAYIKDKRAPDLRKPIRRQIGERIGGE
                     YTAAEREMLNLQFEMTDQIDMINRRLEWMAASALVSGTVTVAGEGYETQVVDFGRASD
                     LTITLSGSDKWPLTVAAGATNTQPSDDIEIWQTTFLKESGSVATDLVFTNKSWRAFRL
                     DTTIKDNAITFPALSPFGNQINAGPQVMKGAIYKGRWGNFDLWLYNDWFIDPLDNVEK
                     PMIPDGAVIMSGADLMGTRAFGVILDPAFNYGPLAYAPKSWVKEDPAQRLILMQSSPL
                     VIPSRVNASLCATVV"
     gene            43469..43879
                     /locus_tag="QBF46_000998"
     CDS             43469..43879
                     /locus_tag="QBF46_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673218.1"
                     /translation="MHPSAQRWSDMAKQPNTGLADDLNAEGSAKDGLSVDDLNAGDNT
                     QEKQPLSKTDDAELSVDDDGGDEKSGDTESQEYVVLKGNCIRHDGEMYRENMRIPVTG
                     KDAERLLQSGVIADVDVLRKRVLASQPSVSVTTG"
     gene            43884..44243
                     /locus_tag="QBF46_000999"
     CDS             43884..44243
                     /locus_tag="QBF46_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673219.1"
                     /translation="MGVDWDSHLLSPLHDVFGDEHEYRPRNGTPFTINGIFDRGYAQV
                     AENLDGDSEINTSSPMLGVRDAEFRKLGKSQPAVSDRVFIKTVGGHIINQLFVVSNVE
                     PDSHGGSRLVLNVVKLR"
     gene            44240..44785
                     /locus_tag="QBF46_001000"
     CDS             44240..44785
                     /locus_tag="QBF46_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ6673220.1"
                     /translation="MNSAAIRQMVVTALTGTTSAGDRVFSPRDWSTSPDMYPVLLVQT
                     PFEQKKSQGRNTPAFTTLTTVRITGRVQEYDGDTVDDGAMRAELALESLREQVERAVI
                     NSYELTRNIQKYAEVRSTINVDSEGEAHMGQLLFEIDIEHYQGPEDFYPVQSVPLEGM
                     DIAVDMPDGTVKPGISLNLQE"
     gene            44789..44986
                     /locus_tag="QBF46_001001"
     CDS             44789..44986
                     /locus_tag="QBF46_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDQ6673221.1"
                     /translation="MFVKPNNGLSVRCPVKGIPLPKEGAEVPDNIFWRRRLSDGDVIL
                     SKKDEGAPEKQSLPKKAGENE"
     gene            44983..46485
                     /locus_tag="QBF46_001002"
     CDS             44983..46485
                     /locus_tag="QBF46_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDQ6673222.1"
                     /translation="MTVPFARVPDNLRVGLFFVEFDNSMANNATATQRTLLIGGMLST
                     GSTLPGIPQRVSSSDTVGELTGKGGILQAMMAAYQKNDTAAEVWILPLEEDSDSMVAA
                     TGTIRVSSAPTATGVISLYIAGERIQLTVVATDTVAAIATSLAAAINAKTTLPVTASA
                     TTDTVTLTAKNLGATGNGIDIRLNFLGLPGGESTPAGLELTITAMSNGVGAPDITGEL
                     ANLQDRTFDFIINPYDDTTSLNVMKEFLSDTGGRWAWDKQLYGHSFGTTTGTYAQLGT
                     KGELRNNQHETLLGVNKSPSPSWAWSAAYTGAAAVSLRNDPGRPLQSLAVQGVLAPEL
                     QDRFELTERNNLLYSGISTFTVDDDGTVRIENLITTYQKNSYGDADDSYLEVETLFSL
                     MFVTRYLRTAVTSKFGRMKLAADGTRFAPGAAIVTPNIIKADQIAEYQTLVWNGYAQD
                     AEAFAKNIIVEQNAKNPNRVDVLWPGTLMNQLRIFALLNQFRTRAESTGA"
     gene            46489..46860
                     /locus_tag="QBF46_001003"
     CDS             46489..46860
                     /locus_tag="QBF46_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDQ6673223.1"
                     /translation="MAGDTTNRLAGTAYVTVNGVTVMVEGSFKYQAATVNRTTLTGMD
                     GVHGYKEKPVAPYISARLRDSGGTNVQGFNQQTNVNVIAELANGKTIIGRSLWTVNVQ
                     EVESEDAVFDVRWEGRDVTEN"
     gene            46862..47140
                     /locus_tag="QBF46_001004"
     CDS             46862..47140
                     /locus_tag="QBF46_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDQ6673224.1"
                     /translation="MAEIERVKTIPLTVALDDAAEKTTYTQLELKAPTLSQAEQFYEK
                     QAASTSLAAMRLLIALVSGTRESVLQPMDFLDFRKCEEYLLSFLTWKP"
     gene            47282..49210
                     /locus_tag="QBF46_001005"
     CDS             47282..49210
                     /locus_tag="QBF46_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673225.1"
                     /translation="MSNSFDFELVASDQVSEAIDRINEAVRDLEPKLDKTKEGLKLGG
                     QETADGLSGFISRLENMSKSARDNVQFIGDMVPPLKMVGELTGKMGALGLAGAAGYGL
                     KQVAYGFREASRQAYNLDVSAKNAGMRVDDFTRLSGAMRILGADSESANASIEGIFKA
                     FNEAASGKNEGVMAAMAQIGAQIQKNSDGSVNTLKTLESIAKIFPTLRPEQQKSAADA
                     LGLTPELLALMRDGERMKKLLAKSDEFGLTVDPALNQQLSEVNGTMNELSASWDGLWQ
                     RSKNKALKTILSDGSVKDGLEGVTDLFTNGDFTGLSHALGFINSNDAEKLRRIQNDKE
                     LYNSLPRSERGQVDAGFMTDAVRKRYDANYRATDSAIQLQNDLSAISQPQSNVARGNV
                     PYGETRNNAIGFRNNNPGNLRAAANATGKNGGFSTFANDADGRAAMARQLMLYGDRGN
                     NTLDGIIHTYAPQSENNTRAYIDSVSKSTGFGAQQRINLHDPDVLKTLMAAMIKHESG
                     AQPYTENELSDSVRTAIIDDRWKGLRSPEVLAQQRYDIISGSQTGNRDSSTLSNPSDE
                     TDILSGSQNRNRESIILSDTGKKSDESVLGDNLAKSLKEAMSEQPLKLEITMVNDKGE
                     RKTYNAENNGRITTAMNY"
     gene            49242..49490
                     /locus_tag="QBF46_001006"
     CDS             49242..49490
                     /locus_tag="QBF46_001006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673226.1"
                     /translation="MNEKVFGAKAIYTPKVFALVYLVIGIFLVFSVVSMNFTAITISV
                     VSALLLRVLYEFLMNSFKATEHLYRIAESLDRNGSSDK"
     gene            49554..50957
                     /locus_tag="QBF46_001007"
     CDS             49554..50957
                     /locus_tag="QBF46_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HDQ6673227.1"
                     /translation="MPSIIQDAITSLLGGDTSDDWQGQLRPSSFRGVPFAIVAEEGSH
                     GRRQAVHEYPYRDTAWIEDIGRATRRFVIRGFLIQNSQVYGGGDAITQRQSLIEACEQ
                     KGSGTLVHPTLGELTVSIPENGLRISGSMENGRVFEFTLMAIESGLKVFAVTGSTVAG
                     ATVKTNYLKLVSTAVLSTIARVKSEIRGVTQAINTIRGTVTFWTNMVDSTISQVTNLS
                     NVLNSTFGNTRYGRYSKGSVGGSSSAVAGKSSVADVDDERALADKVTAQSVMDRKNVT
                     DRSSQLSSSNTPDEFVQGVADVVNAILNSAGSVNDRITALEKLANSISTEYQQSDSSK
                     AISATMNTLIVVLCTGAMTSAAADSRPASTDEAEELTQRVSVQLDTALVLAGDRADDD
                     MYNALLAVRSAFLSTMSERASGLSELLQVTTAQPLPALTLANRLYQDATRADELVQEA
                     RVPHPAFMPTTMKVLRQ"
     gene            50954..52039
                     /locus_tag="QBF46_001008"
     CDS             50954..52039
                     /locus_tag="QBF46_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6673228.1"
                     /translation="MNADSDLDVVSLTVDGKIIEGWDSVRVTRGIERFPSDFDLGLMD
                     YFPGNEDRQLVEEGMSCEVRIGDDLTLTGYVDDWEPALSRSRHEVRATGRSKCQDLVD
                     CSAEWPNNVINASNALEIASRLASYYGITVTTDVDELVKVPQFTLNWGESPQEVIDRV
                     ARWSALLYYDQPDGNLLLTRVGTRRAASGIAEGVNVEQAYYRKSMADRFSDYVGVSMS
                     VSPIAGYSPDTAYDAVTLATARDPEAARMRYRKHISIVESTLMATQQAQSAIDWEMNR
                     RYGRSKQLSVTIDSWRDKDGKLWEPNTLIPVDLPTLRLPKTELLLAEVTYMRDDYGTH
                     ARMTLMPPEAFSVQPYAFYQNLAGFNT"
     gene            52036..52620
                     /locus_tag="QBF46_001009"
     CDS             52036..52620
                     /locus_tag="QBF46_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HDQ6673229.1"
                     /translation="MKQLFKHAATRIAGMLGIGRITAMKDGGVVQSIQYQTPLEVASA
                     PRMAEFGFSSGLPSGTDVVLAFIGGDRSSAVVIASNHQGFRHTGLKAGETVMYNQWGL
                     NILLTEKGIFLDAKGQNVEVNNATNVTINASQGILANTPILRCTGDIVDNCETNTRTL
                     KELRDAHNDHDHVVKNAQSGNDNIRSQKTEDQVT"
     gene            52617..53054
                     /locus_tag="QBF46_001010"
     CDS             52617..53054
                     /locus_tag="QBF46_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDQ6673230.1"
                     /translation="MSDIASFWNVDEMFADWQKGLGELTTGNDLQTAILDSLFTDRLA
                     RADDNYEDSDRRGWWGDSGEESQLGSRLWLLRRKKLTPDVAKKAEEYSSEALNWLKVD
                     GVVSEVIPVARIVLPDRLNLIIRYQAPGKDWQEFRFYWIWEQR"
     gene            53056..54198
                     /locus_tag="QBF46_001011"
     CDS             53056..54198
                     /locus_tag="QBF46_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDQ6673231.1"
                     /translation="MPFKRPTLSELRDGNRKFMQAELEDVGALLRFANLKVLADMDAG
                     MGHLHYAYLDYIALQTNPFTSTDEYLAGWMALKQVFRKPAAAAKSPAVQASGSVDCII
                     PVGSIINRGDGYQYRTDADLKIQADGFGIVAVTAILPDITSDVTGGGARGNADAGTIM
                     TLDANIAGVDPQVTLLSAATGGADIETEEDFRSRGLLAWQNPPQGGSDADYKKWALEV
                     SGVTRAWVKRRLNGAGTVGVYIMCDRNDNGGFPVGTDGISQLEDWGAVKATGDQLAVA
                     DHIYPQQTDTAIVFVCSPIKKVINIEISGIKNADSTTVQGIKDALTALFFDEANPDGS
                     GKVYLSDINGSIGGVSGTTGYILNSPTANITFAVGEIPVLGGVNFV"
     gene            54195..54788
                     /locus_tag="QBF46_001012"
     CDS             54195..54788
                     /locus_tag="QBF46_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDQ6673232.1"
                     /translation="MSLFSKNDYAGALGALLPTGRAWPRSQRTVQAAVLRALGSAFQR
                     SDNDAQSLITGAFPPTATVMLSEWESSLGLPDDCAIGESGGVSDRQRAVVAKLISTGG
                     LNRDYYIRVAAALGYTITITQFRPAMSGMSVCGDALNGDEWPFTWRINAPQTTIKYSL
                     AGASYCGDPLASWGNKQLECSINKIAPSHLNIIFNYS"
     gene            54840..55457
                     /locus_tag="QBF46_001013"
     CDS             54840..55457
                     /locus_tag="QBF46_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673233.1"
                     /translation="MLRIGQVEATATQDGKYTDGSVAGGIAATRLRAAAFNAMQEELA
                     HIVESAGLALDINDMTQVLKAIQKLTLSRANPFADIKSDGAAAISTALTNLGLGEAAK
                     RNVGTGENQIPDMSAYSSGSGWQKLPDGSIEQWGRINFPNNAAAVSTNVTFTIPFTQE
                     PDVVIVYDGGFGGGNMWGATNWTKTGFVAHCNYGFEGGAFYAKGR"
     gene            55460..>55751
                     /locus_tag="QBF46_001014"
     CDS             55460..>55751
                     /locus_tag="QBF46_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ6673234.1"
                     /translation="MKYLYVDNLAYPYALQSVYAAKGQWPEGKGVDIDEAIFREYFHD
                     TPPDGKYRITGDDGMPAWADVPPPTREEQIASAATKKQQLINQANDYMNSRQW"
BASE COUNT        14133 a        13501 c        14708 g        13409 t
ORIGIN      
        1 agggagaacg catataccac ttctgtgcat actgttgagc tgaaaaactg acgaattatg
       61 ataaactcca gccaacttta tttcatatca ttgagggcct gtggctgatg gcacagctat
      121 atttctacta ttccgcaatg aatgcgggta agtctacagc attgttgcaa tcttcataca
      181 attaccagga acgcggcatg cgcactgtcg tatatacggc agaaattgat gatcgctttg
      241 gtgccgggaa agtcagttcg cgtataggtt tgtcatcgcc tgcaaaatta tttaaccaaa
      301 attcatcatt atttgatgag attcgtgcgg aacatgaaca gcaggcaatt cattgcgtac
      361 tggttgatga atgccagttt ttaaccagac aacaagtata tgaattatcg gaggttgtcg
      421 atcaactcga tatacccgta ctttgttatg gtttacgtac cgattttcga ggtgaattat
      481 ttattggcag ccaatactta ctggcatggt ccgacaaact ggttgaatta aaaaccatct
      541 gtttttgtgg ccgtaaagca agcatggtgc tgcgtcttga tcaagcaggc agaccttata
      601 acgaaggtga gcaggttgtt attggtggta atgaacgcta cgtttctgta tgccgtaaac
      661 actataaaga ggcgttacaa gtcggctcat taatggctat tcaggaaagg catcgccacg
      721 attaataaga atttctttac tgacagggtg agcagggcgc ttttatcctg tcagttcgtt
      781 ttacgcactt cttccgggct ataaaccctt cttggcagtt ttttaacgcc gctatacgcc
      841 tcacagggcg cttaaccacc gacgttgact tgtgacctgt aaagtacaat atccctgtgt
      901 ttaggcgtta tacatcgtcg caaatatgat gaaggctaat gctgtcggtt tatggaaaag
      961 ttgctttggg taaacaaaaa aacggcccca gaaggggccg tttatgttgc cagacagcgc
     1021 tactgattaa gcggattttt tcgctttttt ctcagcttta gccggagcgg cttctttttt
     1081 cgctgcagtt tcaccttcta cataatcacg accgtagtag gtatccagca gaatctgttt
     1141 cagctcggag atcagcgggt aacgcgggtt agcgccggtg cactggtcat cgaatgcatc
     1201 ttcagacagt ttatccacgt tcgccaggaa gtctgcttcc tgaacgccag cttcacggat
     1261 agatttcgga atacccagtt cagctttcag cgtttccagc catgccagca gtttctcgat
     1321 cttagcagca gtacggtcac ccggtgcgct cagacccagg tggtcggcaa tttcagcata
     1381 acgacggcga gcctgcggac ggtcatactg gctgaatgca gtctgcttgg tcgggttgtc
     1441 gttcgcattg tagcgaataa cgttacaaat cagcagggcg tttgccagac cgtgcggaat
     1501 atggaactgg gaacccagtt tgtgcgccat tgagtgacat acacccagga aggcgttcgc
     1561 aaacgcgata cccgcgatag tcgctgcact gtgaacacgt tcacgcgcta ccggattttt
     1621 agacccttcg tggtaggacg ctggcagata ttctttcagc agtttcagtg cctgcagagc
     1681 ctgaccatca gagaactcag atgccagtac agaaacataa gcttccatgg cgtgagttac
     1741 tgcgtccaga ccaccgaaag cacacaggga cttcggcatg tccataacca ggttggcgtc
     1801 gacaatcgcc atatccgggg tcagcgcata gtccgccagc gggtatttct gaccagtagc
     1861 gtcgtcagtt acaaccgcaa acggagtgac ttcagaacct gtaccagaag tggtggtgac
     1921 agcgatcatt ttcgctttca cgcccatttt cgggaacttg tagatacgtt tacggatatc
     1981 cataaagcgc agcgccagct cttcgaagtg agtttccgga tgttcgtaca taacccacat
     2041 gatcttcgcg gcgtccatcg gggaaccacc acccagcgcg ataatcacgt ctggtttgaa
     2101 ggagtttgcc agttctgcac ctttacgaac gatgctcagg gtcgggtccg cttctacttc
     2161 gaagaagact tcagtttcaa cgcctgctgc tttcagtacg gaagtgatct gatcagcata
     2221 accattgttg aacaggaagc ggtcagtcac gatgagcgca cgtttgtggc catcagtaat
     2281 cacttcatcc agcgcgattg gcagggagcc acggcggaag tagatagatt tcggaagttt
     2341 gtgccacaac atgttttcag ctcgcttagc aacggttttc ttgttgatca ggtgtttcgg
     2401 accaacgttt tcagagatgg agttaccacc ccaggaacca caacccagag tcagggaagg
     2461 tgcgagtttg aagttataca ggtcaccgat accaccctga gaagccgggg tgttaatcag
     2521 gatacgagcc gttttcattt tctgaccgaa gtaagaaacg cgagccggtt ggttatcctg
     2581 gtcagtgtac aggcaagagg tatgaccgat accacccata gcaaccagtt tctctgcttt
     2641 ttctaccgcg tcttcgaaat ctttagcgcg gtacattgcc agagtcgggg acagtttttc
     2701 atgtgcgaac ggttcgcttt catcaacaac ggtcacttca ccgatcagaa tctttgtgtt
     2761 ttctggtaca gagaaacctg ccagttcagc aattttatag gctggctgac ctacgatagc
     2821 cgcgttcagc gcaccgtttt tcaggataac gtcctgaaca gctttcagct ctttaccctg
     2881 caacagatag ccgccgtggg ttgcgaaacg ttcacgtact gcgtcataaa cagagtcaac
     2941 aacaacaaca gactgttcag aagcacagat tacgccgttg tcgaaggttt tggacatcag
     3001 tacagatgca actgcacgtt tgatatcagc agtttcatca ataacaactg gagtgttacc
     3061 cgcgcctaca ccgatagctg gtttaccgga gctgtatgcg gctttaacca tgcccggacc
     3121 accagtcgcg aggatcaggt tgatgtctgg gtggtgcatc agtgcgttag acagttcaac
     3181 agaaggttga tcgatccagc cgatcagatc tttcggagca ccggcagcga tagcagcctg
     3241 cagaacgata tcagccgctt tgttggtggc atcttttgca cgcgggtgcg gggagaagat
     3301 aatggcgtta cgggtcttca gactgatcag cgatttgaag atagcagttg aagtcgggtt
     3361 agtggtcgga acgataccgc aaataatacc gattggttca gcgatagtga tggtaccaaa
     3421 agtgtcgtct tcagacagaa caccacaggt tttttcatct ttataggcgt tgtagatata
     3481 ttcagaagca aagtggtttt tgatcacttt atcttcgacg atacccatgc cggattcggc
     3541 aacggccatt ttcgcgagtg ggattcgagc atctgcagca gccagagcgg cggcgcggaa
     3601 gattttgtct acttgctctt gagtgaaact ggcatattca cgctgggctt tttttacacg
     3661 ctctacgagt gcgttaagtt cagcgacatt agtaacagcc ataatgctct cctgataatg
     3721 ttaaactttt ttagtaaatc atctgctcga atacgagagt atagtcagtg cggtgatgat
     3781 ttgcttaacc gatgaaaatc aaaagcttac tcgcgctcac actcactgtg atttactaaa
     3841 agagtttaaa cattagagtt attatctcta atgcgtcact tccaggtggc gtaagcaaga
     3901 ttactcactt ctgggtactg attacgtgat ccaaatcaaa tttttgcaaa gctgacacct
     3961 ttcagcatcg cttttcgcca ttatagctaa cagttaataa attgtagtat gatttggtgg
     4021 ctacattagc atgttttgca caactagata acaataacga atgatagcaa ttttaagtag
     4081 ttaggaggtg aaaaatgctg tcaaaaggcg tattgtcagc gcgtcttttc aaccttattt
     4141 atggctaaca ttatccggct tttgcttcgg agctaaccgt gattcagacc ctttttgatt
     4201 ttcccgttta cttcaaattt ttcatcgggt tatttgcgct ggtcaacccg gtagggatta
     4261 ttcccgtctt tatcagcatg accagttatc agacagcggc agcgcgaaac aaaactaacc
     4321 ttacagccaa cctgtctgtg gccattatct tgtggatctc gctttttctc ggcgacacga
     4381 ttctacaact ttttggtata tcaattgatt cgttccgtat cgccgggggt atcctggtgg
     4441 tgacaatagc gatgtcgatg atcagcggca agcttggcga ggataaacag aacaagcaag
     4501 aaaaatcaga aaccgcggta cgtgaaagca ttggtgtggt gccactggcg ttgccgttga
     4561 tggcggggcc aggggcgatc agttctacca tcgtctgggg tacgcgttat cacagcatta
     4621 gctatctgtt tggtttcttt gtggctattg cattgttcgc tttatgttgt tggggattgt
     4681 tccgcatggc accgtggctg gtacgggttt tacgccagac cggcatcaac gtgattacgc
     4741 gtattatggg gctattgctg atggcattgg ggattgaatt tatcgttact ggtattaagg
     4801 ggattttccc cggcctgctt aattaattcc tttcaaatga aacggagctg ccatgctccg
     4861 tttacttcgt cattattttt actttgttcc cgcgcagtta tcaaaagcaa aaggaatagg
     4921 taaaaatatt cttctcaaat tacagttagt tataaggatt tccttaactg cttctcctca
     4981 ccatcatgtt attttcgcca catcataatc ctgggcttgc tgaagaataa ttgaaatgat
     5041 attagtaatt ccactgcctt tggtagagga aagtgctaaa taataatcaa ttgttaaatt
     5101 attgtgcatt tcactgctgg aactgtaatc agaaaagata gacatgctta accaatctct
     5161 atttgattga atttaaagat gtttgttaag acatggaagc aagctataga ttctgatacg
     5221 gtcaataaaa gagaattgct taacaatttt gcaaaatgta ttggcgagta agaagcgcat
     5281 ttggtacttt ccgggcaacc gccaggcgat tctttattgg taatgagaat aattaacaat
     5341 taaagagcgt cgcgaaagaa taatgtgtct cgacagggga gacacagtac gaatcgacat
     5401 aaggtgatcg tctgaatcac cagaataaat aaagtcggtg atagcaatcc gtaacgataa
     5461 agtaacctga cagcagaacg tctccgagcc tgtgcagggt cccattccgg gattacacat
     5521 gctggttaat accagtaatt ataatgaggg agtccaaaaa acaatgacca acatcaccaa
     5581 gagaagttta gtagcagctg gcgttctggc tgcgctaatg gcagggaatg tcgcgctggc
     5641 agctgatgta cccgcaggcg tcacactggc ggaaaaacaa acgctggtac gtaacaatgg
     5701 ttcagaagtt cagtcattag atccgcacaa aattgaaggt gttccggagt ctaatatcag
     5761 ccgagacctg tttgaaggct tactggtcag cgatcttgac ggtcatccgg cacctggcgt
     5821 cgctgaatcc tgggataata aagacgcgaa agtctggacc ttccatttgc gtaaagatgc
     5881 gaaatggtct gatggcacgc cagtcacagc acaagacttt gtgtatagct ggcaacgttc
     5941 tgttgatccg aacaccgctt ctccgtatgc cagttatctg caatatgggc atatcgccgg
     6001 tattgatgaa attcttgaag ggaaaaaacc gattaccgat ctcggcgtga aagctattga
     6061 tgatcacaca ttagaagtca ccttaagtga acccgttccg tacttctata aattacttgt
     6121 tcacccatca acttcaccgg tgccaaaagc cgctatcgag aaattcggcg aaaaatggac
     6181 ccagcctggt aatatcgtca ccaacggtgc ctatacctta aaagattggg tcgtaaacga
     6241 acgaatcgtt cttgaacgca gcccgaccta ctggaacaac gcgaaaaccg ttattaacca
     6301 ggtaacctat ttgcctattg cttctgaagt taccgatgtc aaccgctacc gtagtggtga
     6361 aatcgacatg acgtataaca acatgccgat cgaattgttc cagaagctga aaaaagagat
     6421 cccggacgaa gttcacgttg atccatacct gtgcacttac tattacgaaa ttaacaacca
     6481 gaaaccgcca ttcaacgatg tgcgtgtgcg taccgcactg aaactaggta tggaccgcga
     6541 tatcattgtt aataaagtga aagcgcaggg cgacatgccc gcctatggtt acactccacc
     6601 gtatactgat ggcgcaaaat tgactcagcc agaatggttt ggctggagcc aggaaaaacg
     6661 taacgaagaa gcgaaaaaac tgctggctga agcgggttat accgcagaca aaccgttgac
     6721 catcaacctg ctgtataaca cctccgatct gcataaaaag ctggcgattg ctgcctcttc
     6781 attgtggaag aaaaacattg gtgtaaacgt caaactggtt aaccaggagt ggaaaacgtt
     6841 cctcgacacc cgtcaccagg gcacttttga tgtggcccgt gcaggctggt gtgcagacta
     6901 caacgaacca acttccttcc tgaacaccat gctgtcgaac agctcgatga ataccgcgca
     6961 ttataagagc ccggcctttg acagcattat ggcggaaacg ctgaaagtga ctgacgaggc
     7021 gcagcgcaca gctctgtaca ctaaagcaga acaacagctg gataaggatt cggccattgt
     7081 tcctgtttat tactacgtga atgcgcgtct ggtgaaaccg tgggttggtg gctataccgg
     7141 caaagatccg ctggataata cctatacccg gaatatgtac attgtgaagc actaatggca
     7201 atacgtgggg caggagtgtc ctgctccacg gtgtctgatt tttatcgcat tacagaaggc
     7261 acaggccaga aggtagggca atgttaaaat ttattctacg tcgctgtctg gaagcgattc
     7321 cgacgctatt tattcttatt actatttcgt tctttatgat gcgcctcgcg ccgggaagcc
     7381 cttttaccgg cgaacgtact ttaccgccag aagtgatggc caatatcgaa gcgaaatatc
     7441 atcttaatga tccgatcatg acacagtatt tcagctacct gaaacaactg gcgcatggcg
     7501 atttcggtcc atcgtttaaa tataaagatt attcggtcaa tgacctggtg gcatccagtt
     7561 ttcccgtttc tgccaaactg ggagccgcag catttttcct tgcggtaata ctgggtgtta
     7621 gtgctggcgt tattgccgca ttaaaacaaa acaccaaatg ggactatacc gtgatggggc
     7681 tggcaatgac cggggttgtt atccccagtt ttgtggttgc accattatta gtcatgatat
     7741 ttgcgatcat tttgcattgg ctgccgggcg gtggctggaa tggtggggcg cttaaattca
     7801 tgatattgcc gatggtggcg ttgtcactcg cttatatcgc cagtattgcg cgtattaccc
     7861 gtggctctat gattgaagta ttacactcca actttattcg tactgcccgg gcgaaagggt
     7921 tacctatgcg gcggatcatt ttacgccacg cattaaaacc tgctctgtta cccgtgctct
     7981 cctatatggg ccccgcattt gtcggcatta ttaccggttc tatggtcatc gaaaccattt
     8041 atggtttgcc ggggattggg caactgttcg ttaatggcgc attgaaccgt gactattcct
     8101 tagtgttaag cctgaccatc ctggttggtg cgttaaccat tttgtttaat gccattgtcg
     8161 atgtgctata tgcggttatc gacccgaaaa tccgttactg atactggagc tcgcgatgat
     8221 gttaagtaag aaaaacagcg agacgctgga aaatttcagt gaaaagctgg aggtcgaagg
     8281 gcgcagcttg tggcaggacg cacgtcgacg ttttatgcat aaccgtgcgg cggttgccag
     8341 tctgatagtg ctggtgctga tcgcgttatt tgtaatcctg gcaccgatgc tttcgcagtt
     8401 tgcctatgac gatactgact gggcgatgat gtccagcgcc ccggatatgg agtccggtca
     8461 ctactttggt actgactcat ccggtcgcga cctgcttgtg cgcgttgcga ttggcgggcg
     8521 tatctcactc atggtcggtg ttgctgcggc actggtggct gtggtcgtcg ggacacttta
     8581 tggttcgctt tccggttatc tgggcggtaa agtggattcg gtaatgatgc gtctgctgga
     8641 aatcctcaac tccttcccat tcatgttctt cgtcattttg ctggtgacct ttttcggtca
     8701 aaacatcctg ctgattttcg tggcgatagg catggtttcc tggctggata tggctcgtat
     8761 tgtgcgtggg caaaccctga gtctgaagcg caaagagttt attgaggcgg cacaagttgg
     8821 cggtgtatcg acatcgggca ttgttattcg ccacattgtg ccgaatgtac tcggtgtggt
     8881 ggtggtctac gcatcacttc tggtgcccag catgatcctc tttgaatctt tccttagctt
     8941 cctggggttg ggtacgcaag agccgttaag cagctggggt gcattgctga gtgatggcgc
     9001 gaactcgatg gaagtctctc catggttatt gttgttccca gcgggattcc tcgtggtgac
     9061 gctgttttgt ttcaacttta tcggcgatgg cttgcgtgat gccctcgacc cgaaagatcg
     9121 ttaaggagtg cagccatgag cgtaattgaa actgcaactg tgccgctcgc acaacaacag
     9181 gctgacgcac tgctgaacgt gaaagatttg cgtgtcacct ttagtacccc ggacggcgac
     9241 gtcacggcgg tcaatgattt gaatttttcc ctacgtgccg gagaaacgct gggtattgta
     9301 ggtgagtctg gttcgggtaa atcgcaaact gcatttgcgt tgatgggcct gttggctgcc
     9361 aacggacgta ttggcggatc ggcaaccttc aatgggcgtg aaatcctcaa tttgccagag
     9421 cgtgaactca ataaactgcg cgctgaacaa atctcaatga tttttcagga cccaatgact
     9481 tcgttgaatc cctatatgcg cgtcggtgag cagttgatgg aagtgctgat gctgcataag
     9541 aacatgagca aagctgaggc gtttgaagag tcggtgcgga tgctcgatgc ggtaaaaatg
     9601 ccggaagcgc gtaaacgcat gaaaatgtac ccgcacgaat tttctggcgg catgcgtcag
     9661 cgagtcatga ttgcgatggc cttgttatgt cgacctaagc tgctgattgc ggatgaacca
     9721 acaactgcgc tggacgtcac cgtacaggcg cagatcatga cgctattaaa tgaactgaag
     9781 cgggaattta ataccgccat cattatgatc acccacgatc ttggcgtggt ggcggggatc
     9841 tgcgacaaag tgctggtaat gtacgccggg cgcacgatgg aatatggcaa cgcgcgcgat
     9901 gtcttttatc aacccgttca tccttattct atcggtttgc tcaacgcggt gccgcgtctc
     9961 gatgcggaag gtgaaacaat gttgaccatc ccgggtaatc cgccaaacct gctgcgatta
    10021 ccgaaaggtt gcccgttcca gccacgttgt ccgcatgcga tggaaatttg tagtagcgca
    10081 ccgccgctgg aagagtttac gcctggccgt ctgcgtgctt gctttaaacc ggtggaggaa
    10141 ctgttatgaa tgctgtaact gaaggaagaa aagtcctcct tgaaatcgcc gatcttaaag
    10201 tgcactttga aatcaaagat ggcaaacagt ggttctggca accgccgaaa acgctcaaag
    10261 ccgtcgatgg tgtcactctt cgcctgtatg aaggggaaac attaggtgtg gtaggggaat
    10321 cgggatgcgg taagtccacc tttgctcgcg ccatcatcgg tttggtcaag gcgaccgacg
    10381 gtcatgttgc ctggttaggt aaagagttgc tgggcatgaa acccgatgaa tggcgtgccg
    10441 ttcgcagtga tattcagatg attttccagg atccgttggc atcgctaaac ccgcgtatga
    10501 ccatcggcga gatcatcgct gaaccactgc gtacttatca tccgaaaatg tcacgccagg
    10561 aagttcgcga gcgcgtgaag gcgatgatgc tgaaagtcgg gttattgcct aacctgatta
    10621 accgctatcc gcatgagttc tctggtgggc agtgccagcg tatcgggatt gcgcgtgctc
    10681 ttattcttga accgaagctg attatctgtg atgagccggt gtcggcgctg gacgtgtcaa
    10741 ttcaggcaca ggtggtcaac ctgctccagc aattgcaacg tgagatggga ttgtcattaa
    10801 tttttatcgc tcatgacctg gccgtggtaa aacacatttc cgatcgtgtg ttggtgatgt
    10861 atctcggcca tgcggtagaa ctggggacct atgatgaggt ctaccacaat ccactacacc
    10921 cttacaccag ggcattgatg tcggcagtcc ccatacctga tccggatctg gagaagaaca
    10981 aaaccatcca gttactggaa ggggaattac cgtcgccgat caacccgcct tccggttgtg
    11041 ttttccgtac ccgttgcccg attgccggtc cagagtgcgc caaaacacgt cctgttctgg
    11101 aggggagttt cagacacgcc gtttcttgcc tgaaagtcga tccgctttaa aaacaataag
    11161 ggctgacagt tgtcagccct ttttcacgct aaaagcgatt atttattccc gccagatgat
    11221 atggcaaagt ttgtgatctt tttcgcggca taacagaatg cgggcaaaaa catcgttgat
    11281 ttcaccatct tcactgtccg ccagaccaat caccacttcg gcaaaaaagt ccgggttcaa
    11341 atcgaaatca acatgttcct gccagtcttc cgccggatcg aataactccg cgccgccgcg
    11401 ctcttcaaac tgaagattga acagcagaac atcagctgga tcgagattgt cggcagccag
    11461 ttcgagaaaa atatcgtagg cctgttcaag cgtttcatct tcagtcaggc gattgtttag
    11521 atccatatcc atagttacta cctgtttaac ctctgttggc gacgttttac agcaacggac
    11581 tgaagaagta aaacagtcgc tcggcgacac gttgccataa tggacgtttt agccataaac
    11641 gggcatcgag cagacgtgaa cgcgaaatat aatcgtcctg aacggcggcg aggtcagcac
    11701 caaaaccttt atcgtcgatt gccagggtaa tctcgaaatt tagccacaga ctacgcatat
    11761 caaggttaac tgtgccaacc agacttagtt cgccatcgac cagcacgctc ttggtatgca
    11821 gtaacccgcc ttcaaactga taaattttaa ccccagcagc cagcagttcc gtaaagaatg
    11881 cgcgactggc ccagccgacc agcatcgagt cattttttcg cggaaggata atactgacat
    11941 ccaccccgcg ctgcgccgcc gtgcaaatcg catgaagtaa atcatcgctt ggcacaaagt
    12001 agggcgtggt catgatcaaa tattcacgcg ccgaataagc cgcagtcaat aatgcctggt
    12061 gaatgagatc ttccggaaag ccggggccag aagcaattgt gtgaatggtg tgaccgctgg
    12121 cctgttcaaa cggcataata ttgacatctg gtggtggcgg cagaatacgt tttccggttt
    12181 caatctccca gtcgcaggaa taaataatcc ccatcgcggt ggcgataggg ccttccatac
    12241 gcgccatcag atcaatccat tgccctacgc ccgcatcttg tttgaagtag cgaggatcga
    12301 ccatattcat gctgccggtg tacgcgatgt aattatcgat catgatcatc ttgcgatgtt
    12361 ggcgcaggtc catacggcgt aaaaacacac gcatcagatt gacctttaag gcttcgacca
    12421 cttcaatacc ggcattacgc attagctcgg gccacgggct gcggaaaaaa gccacactcc
    12481 cggcggagtc gagcatcaat cggcaatgaa tgccgcgtcg cgcagccgcc attaatgatt
    12541 cagccacctg gtccgccatg ccgccgggct gccagatata aaacaccatc tcaatattat
    12601 ggcgcgcgag ctggatgtcg cggattaacg cctgcatcac atcatctgac tcggtcatca
    12661 gttgtagctg attccctttg accccagcga tcccctgacg acgctcgcaa agcttgaata
    12721 atggcgcagc gacactgcta ttttcttcgg cgaagatatg cttacaggct ttaaggtcgt
    12781 taagccattt tgcggtggaa ggccacatcg ctctggcgcg ctcagcgcgg cgtttgccta
    12841 aatggagctc gccaacggca agataggcaa taattccgac taacggcaga atgtaaataa
    12901 tcaacagcca ggccatcgcg gagggaactg cgcgtcgttt cattagaatg cgtaaagtta
    12961 cgcctgcaat gagcaaccag tatcccagaa tggccaacca actcaccaac gtataaacgg
    13021 ttgtcataga ttagaaatcc ttttgaaagc gcattgttat gagtttacgc atatggattc
    13081 atctggcaaa taaaaacgcg ggaaaagcac tggtcagcgt cgggggtggg ggtataatga
    13141 ccattctgtt attgcataga gtagttaaca tgaagcggag tagaacggaa gtggggcgct
    13201 ggcgcatgca gcgtcaggct agccgacgta aatcgcgttg gcttgagggg caatcgcgcc
    13261 gaaatatgcg tatccacagc atcaggaagt gcattctaaa caaacagcgt aactcgttat
    13321 tgtttgcgat ctacaatatc taaatgtact gggcaccatt gcggtgccca ataagtttac
    13381 tcttccgtct ttaaactcta tttttgcgcc gattctttac tgtcagcttt cgtttctagt
    13441 tcatcgttat cgctgaaaat accatgacct gagttcaaca acattgaaac gagcatatca
    13501 ttattaatct cttcaccatt taaaactcgc gccagaattt cgctgccaaa cagttgcggt
    13561 gagagaatga tatccggatg taccatctta atcttattca ggtttttgct atcactgacg
    13621 gcgagaactg ttttgacatc actgctcata tcttttgccg agagtacaac aaacgcgtta
    13681 tctgcatcgt tatcactcag cgccagaatg gcccggcaac gatcgattcc cgctttcttt
    13741 aatacagaac tgtcattact gtcaccgggg ataacatcag cattatcgcc taagcgttgc
    13801 tcaagttgct tgatatcatc ttcaggcaag ttgctgataa ccgttacgtt ttgtccgcgt
    13861 tgattcagtt gcaggatcgt attgatggcg agaatcgaat gtccgcaaac aataaaatga
    13921 tctttacgat gcattgtatg attgtttcct tttacaagtt tgttgaatcc cccgcggata
    13981 agcggaccaa aaattgaggt catagatgtg gcaaaaacgg taatgccgga aataatgacc
    14041 gaaatagtga acaatcgtgc tgattcagaa acagggacaa tatcgccata gccgacggtt
    14101 gacatggttt ctatcgaaaa atagaacgcg gtcatcaaac tttctattcg cggattaaaa
    14161 ccttcgctta aataaagcgc accgtaggtt gagtaaaaca gtaatgtcgt gaaactaata
    14221 aatgcaaaaa ttgtcccggc tgcggcgcta ctgtgggaga agtctttgcg cagtatcagc
    14281 aaaaacacca gcgtaaaaat gcaaaatcca atactaaatt tcagccaggg ataaaaatgc
    14341 agggtgtaaa ttagcgctat caacaacaaa atgatactga ttgcccatgc gagctttgcc
    14401 cggaacagta agccaatgga gttcaggaca agaaagacgc cgagcataaa gagtggggcg
    14461 tttgccagca aggagagatc caactctgaa aatgctttga tatggaaaat atcaagcaaa
    14521 ttgaccgaca taccatagat tgttttaaaa ataagcaatc catttaaaaa gacggccagc
    14581 gcgagaatgt cgtgccgaag ggtcacccat aaatttgttg ctgtttgttt gaatgtagcc
    14641 cagtgactca ctgatacctc tgtaaccaca attttgttta attatcttag cgtgcacatt
    14701 ttctgaacat acatgcagcg cgtgaatgtg ttaaacaata gtagatgact ttatccgcag
    14761 taatcaaagg atttgagcga gcaactgtac ctcaggaaag gtcatttttc ctgaatatgc
    14821 tcacatcata taaagaaata cagataaagt tattatctgc ttgtggtggt gaatgcactg
    14881 accggctata aggaaaggcc aaacaagaac acggttgcaa aaaccgtgcc cttaaatatt
    14941 gaatctctat tcagaacact ttcttaaatt gtcatttggc atattacgaa caattccgcg
    15001 taaaaacgtt ctgttacgct aaacccttat ccagcaggct ttcaaggatg taaaccataa
    15061 cactctgcga actagtgtta cattgcgtgt agctttgagt gggcaacttt gtgtacactt
    15121 ttgtgtaccc aaaaacaaaa atgtgtaccc attcaatgat caccgacaca aagctcagga
    15181 aggcgctcgg caagaaaaga gatgatatcg agattatttc tgattcgcac gggctcaacg
    15241 ccagaatcag ccaggccgga aaaatatcat ttttctatcg gtatcgctgg gccggtaaag
    15301 cggtaaaact caatgttggt gattatcctg caatgagtat cacccaggca agagagcgtc
    15361 gccaacaatt cagaaactgg ttaactgagg gactggatcc gcgagagcag gtgaagctgg
    15421 ataagcagac ccgacaggaa gcgatgtccg ttgccgaagc gttcaattac tggattgaaa
    15481 ggcactgtat cgctaacggg ctagttaaag tcgattacta tcgccaggtg tttgagaaac
    15541 atatcgccga accgatgaag aatgtcaaag tcgataacac agcgaaaatg cactggatca
    15601 acgtcttcga ttctatagaa agcagggtga tggctcatta catgctttcg ctgtgcaaac
    15661 gggcgtttag gttctgcgtt aacagaagtg tgatcgcctc aaacccactc gagggattac
    15721 tgccatctga tgtcgggcaa aagcctaaaa agagaactcg caggatggac gatgacgatc
    15781 tgcgcaaaat ctatcagtgg ttgaaaagcc atatgtcgat agagtccgtt ttcctggtga
    15841 aatttattat gcttaccgga tgccgtacgg ctgagattcg acttagtgag agatcatggt
    15901 ttcgattgga tgataatgag tgggtcgtgc ctgcgggcag ttataaaact cgggtacata
    15961 ttagaagggg actctcagac gccgccgtta acctggtcag aaatcacctc aagaaaataa
    16021 acaccaatca cctggtgact tcacaacgta aaattgatgg cgggatcaaa gattcgcccg
    16081 ttcattcacc tgtggcatcc aattacgccc gttctatttg gaatggaaca ggtatggcag
    16141 agtggtcgct tcatgatatg aggcggacga tagccacaaa tctctctgag ttaggttgcc
    16201 cgccgcacgt aattgaaaag ctgctcgggc atcagatggt gggggttatg gcgcattaca
    16261 accttcatga ctatatcgat gatcagaaac actggctccg cgtttggcag agccatcttg
    16321 aagagatcat cggagagccc ttcagttaat ttatcttctt tttatcctcc cactctttga
    16381 ttgactcaga gcgccagcgg ttagggttgc cgggccagtc agggggtggg aacgggcata
    16441 cgaagccccg aggcattgtg tctgcacttt gccatgacca aagggttttg cgtgaaattt
    16501 tgtagcgact ggtcaggtct gacgttacca aaatatcatc catagctctc tccagttgcc
    16561 cgttcgggcc attcaaaatc tttttcaacc aacctgcccg ggcagggagc ggagacggcg
    16621 catgccggtc atcgctgtgg ccacgtagct cgcctttcgg ttcaccacct ccacccagac
    16681 tttcacgcct tcaaccttca ccgtataagt ctctttcatc ttgcttcgcc catagtcgcc
    16741 atatgtttgc aagtgagccg ccagcgcaac atcgcaagcg cggcgaccaa taggtgattg
    16801 cttactgcga ttaatcagct tcatcatcac tgcactccca aagtggctac gacatcactc
    16861 gctgtttcgc gggtactgcc tttgctggat atagcccggc gagcactgac gcggtgcagc
    16921 gtgaagccgt gctgttcgta aagttcaatt acgcgcggag cggtagaatt gctgatcacc
    16981 acttttgccc cccgctggtg ggctgccaca cagctttccg taagttctac ctggctatcc
    17041 catgagaacc caccagccgc atagttagtg aaaccagcgg tgccgggcag cggctcataa
    17101 ggcggatcgc agtaaacgac gtcaccatca cctgccagcg cgagcgtgcg cctgaagcct
    17161 gcattcatga atacgcatgc gtgagccttc cgcttaaatg ccctgatctc ttcttccggg
    17221 aaatattgcg ctttatattt cccaaagccg acgttaaaaa aaccgtccag gttataacgg
    17281 atcagcccgt tgaagcagtg ccggttgagg taaaggaacg ctgctgcgcg ctcgaccgca
    17341 tccagccgct gcgcgttgaa tgcttcacga attaccgtgt agttttcggc atcattcaga
    17401 tgcctgaatg ccttcattgc ctcatagatc accgagtcgg ggaccaccgc cagcatctga
    17461 tacaggttaa tcaggtcagc gttgacgtca gccagaagaa agcgttcgtg cttgtctgag
    17521 ttaaggaaca ccgagccgcc acccacaaaa ggctcaataa ggcgtttacc tgcggggatc
    17581 aaacgatcca gttccggcag cagcgaatat ttgccgccag cccatttaag gaacgggcgc
    17641 tgccagctgc gcagcgccgg ttcttctatg ggcatcgccg cagctccact gcaaacagag
    17701 ccgtatctca tgctgccacc tgcttttcgt taagttcttc agccagtcgt tgcgccttca
    17761 gtgggttggt aacgacttca ccccacggca gcaaccatcc gtttttctct ttgagccagg
    17821 gaaggcgcac cgcgccaacc ctgatgtcgt cgtgagcgtg agtcatagga tggactccat
    17881 ttcgtcgatg tagaggccct gagcaatcag gcggctacgg cgggcggcac gggctatgca
    17941 ctcctgccgt ctaccttcct gcgattgctc aatggcgcgc cgggtgaaca ggcgcgattt
    18001 gccctgcggc gtcacaacct tcggcttcgt gaccaggtcg aaagtccggt cgcagattcc
    18061 gtcctcgttg agccattttt ccgactcaac gatctgagct atctgtccgg agccgcgggt
    18121 gatcccgttg gcgacccggt taaactcaat gagcgttacg ccaaacttct cagcgatttc
    18181 actaccggtg accgggcggc cgcgcgtctg aatcatccag ataacgcgct cacggaggcc
    18241 ggagaattgt ccggttcgcc cgggcctgcg atagaagggg gtgcgtttca ttcgagctcc
    18301 agtatgcggc gcttagtgtc cgcaacaagt tcgaggaaat catttctgcg tgcgcgaagc
    18361 cgggctattt ctgtttcaca ttcagcagct gtaaggcgat aaacgatgag ctgcttaccg
    18421 tccgggaagt ctgagcagta gctgatgaag tccacccaat ccctgccgga gcaatcaagg
    18481 tgaccgacca gttgccatct gtatgccgga tcgaaggagc cgcgggttag ggtggagtag
    18541 tgagtggcgg caatgaccga cttaatctca acgagcccgt cctggccaac gagtccgtct
    18601 gggctgtcac cgtaggtttc gtgatcaaag aacccaccgt tatccacgtc gacgaagttc
    18661 atctcttcgt acagcatgcg ggcaattggc tcctgttcgt gcccgcgctc catgtgctcg
    18721 tttgaaaagc caaattcaga cttgcacccc ttaatctgct caagagccaa ttgaagggca
    18781 taacgcttgg ctggttcgcc aaaagccttc ccatcgttag ccatgatcag gccgaagttt
    18841 gaagcggtgg ccattcggtg agtgggcggc gggtcgcatc gcgcagctcg tcgcaggcat
    18901 caacgaatcg cttaatttcg gcctcagccg gacgcacagc ctctttcagg cgcttcaggt
    18961 actcacggcc cggcttttcg attgccgtct tgctgcggga cacctgcgct gccagagagg
    19021 caacacggtc acggcctttc ttcgtggaca ggtccggcac ttcgtttaca gcctggcgga
    19081 tttgctcaag gtacgcgtca aggccgcccg ctacgtaaag cactggggcc tgttccggct
    19141 tgatttcgat gacagttaag tccgttactt cgctcatggt ttctcctgaa atttggatgt
    19201 gcagatcccg cccgcgtaat gccaggccga tcggttgaat agggtggtta ctgctgcgcg
    19261 atggatttcg ccgggaactc gccgttgcgg atgatgcttt ctaccggcca gcactcagct
    19321 gacactttct gctctgtagc tgccaggctg cattcctgct ggctgtcgta aacgccgaga
    19381 atgacatcct gataatcacc gttggtcatt gccacggtca ggacgagtgc gaataaagtt
    19441 tccatcagtg aagagtcctc ccgatggcga cggcgtaaag gcgttttgct tcttcccacg
    19501 ccggagcatt gcgatggagc accgcgaacg acgcgagccg ttgggcctct ctgatctgct
    19561 gctggtttac catgatttcc tcttggcctt atcgcggcga acggaacggt taatacaaga
    19621 cttcaacgca tttattcagt gtttcaatgg gcggtggatg gccgccggtt gtcataactt
    19681 gagccactcg taaatgactc caggtatgaa aaagccgctg gttaggcggc tattgaggtt
    19741 cgcgcggctt gtggtcgaat cggtgccacc cgtcagttaa ttcaaatgga gcataacttt
    19801 cccggcgctc agcgaaccca agctcaacag acagagcgtg cagctcatgc cgacgcttaa
    19861 tctgttccat cgcaatccag tcagcatcgg cattgcgctt ttgagcttgc ttcggcgaca
    19921 ggtctaaact gtttatttca cttagtttct gctgacgctt gatgtcctgt ttcatattgc
    19981 gaagcgcatt tatcattgag tcgatgcgct tgatgtcgtc gatcatcccc ttaccctctg
    20041 tcgttacccg ctgatgcggg agaaatgctt tggcgatgtg gtaggtggga gacccatttc
    20101 gacccgattc ggcctactta tcttcggcaa tagctcctcg ggcctcgccg ctttacgtgc
    20161 gacatattcc cgtccatgaa cccttcacca catcccataa cattccctgt attggtcagc
    20221 gccaactccc tgccagtgtt gcccgttctc acgccgttct cgctctcgcg cggggatact
    20281 ctctcaccga ccggatcgca cccagtgata cagcacgttt acgtgtaggg gtcttaacag
    20341 gtcattgacg ctgtaaatct gcatgttgtt aaaaagcagg cgacttgctg tccgccgctg
    20401 gctaacttcg ctcagctgtc gatgtttcgt ttcgatgggg taaatttagc gtgatgctaa
    20461 attatgcgca atagcaaaat gctaaattgt tgaatggttt tatttagcgt attgattaat
    20521 aagcgattaa aaatttacag cgaaggaatt cgggacgtaa aaaagcccgc gcgatggcgg
    20581 gcttgagggg ttttgcgtga ggttatggga tgtttagtat tttggcatca accacaacgc
    20641 cgatgatttt gcagtttcca ttaatttcta gcattggata tgcggggtta aggggcttta
    20701 ggaagcgtct gccggcatcg attacaagct tcttaaaggt cgcttcgtta tcgccttcta
    20761 gcttcgcgac aaccagcttt ccgttgcgcg gctcgacttc aggatcaaca agtatcgctg
    20821 ctccctcggg tatgctcagt ccagccgggg aggtcataga atccccttta acgtccagcc
    20881 agaatgaatc ttctgagcag tcaacagtcg tgtcatacca gcgatctatc gctcttcggt
    20941 gataaggttc tacagcttcc atccattgcc ccgcgcttac ccagctgatt acaggataac
    21001 ttccttttgt ctcgttcagt cctcgaaatg caacgttcga aggttcttca ctggcgtgta
    21061 aaacatccat ccagccaaaa ggcagatcaa gcgcagtttc aattttgcga gccatcttat
    21121 cgccgatatt gcgatgaggg tttggtccca gtagctggct aagcgcagcc ggacttgtct
    21181 cgatgagctc ggcaaactgc gctttggtca ttccagactc gtgctgacgc ttctcgtaca
    21241 gcgcttccag gttggctttt ctgatttctt tattttccat acctgcattg ttactgcttt
    21301 tagcaaaatg ataaatgtgc aaattgctaa atgatgcttg cgtagtattt agcataacgc
    21361 taaactccaa atcaaacgac tcacccggag acaccaatga gcactgaact acaccgctgg
    21421 cgcaaggccg ccactaccga cgaatgggcg cagctcgcaa agttggctaa cacgacgcca
    21481 ggttacctgg accagatcgc ctacggaaat cgccgggcat ctccagaaat ggcatctgct
    21541 atcgagaaag gcacgaagaa ttttcaccgc caggctccgg tcctaaaaga aagcctggta
    21601 ttcgcatcgc cgcgtgatac tgcggcctaa ccacgaaagg gaaagcaatg cattcacttg
    21661 cgtatcaaca aggtaacaaa ttttcgccaa cggcgatgat ttaccagaat cgccgggagc
    21721 ctgattccgc ggcgttaaac atcgatggga tccgcgctgc tgttcgcgcc tgggcagctg
    21781 attgccgaag ccgtgaattt gtcgccgcgc tgatcgtgga agagtggcgg gcgtccggcg
    21841 gaaccggtct ggatatcccg actgactcgc accgccagat gcagaaagtg tttcgatgga
    21901 ttgatggcga taccgaatac gccgccaaca acattcgcca gctggccccg gcaatcatgt
    21961 cggtcctgcc gctggagtac cgaaaccgtc tggcgcagca gaacgacacg atgtcgctga
    22021 tcgcctctgc gatgaaagag tgtgccgagg ctaaacaggc cgtgctgctg gacgctccag
    22081 agcatcagaa gctcaaagag gtaagcgagg gtatagcgtc gctgttccgc ctcatgccgg
    22141 agcaggtagg accgctgatg acgatggtta catcgatgct gggggttata tgagaggcac
    22201 aagaaaagaa aaagcccttg aagcggtaac ttcaaaggcc cttatcacac tgtgttacgg
    22261 caagtaacgg gagtaagtat gtcaaacacc gctgaaataa tcaatttccc aaataaaacc
    22321 gaacaaccgg gaggtcgtat ggccgacctg tcgaacgggt ataccaaggt cgctaacgag
    22381 atccaacagc ttaagcctcg cctgagactg tcaggccggg aatggcaatg ttttgaggcg
    22441 gtgatctggc ttacctacgg ctggaacaag aaacaggacc gcgtgacaaa tacggttatt
    22501 gccgagctta cgggcctgag cgatacgcat gtatcggacg cgcttaagtc tctcgcagaa
    22561 cgcaaaatca tcttttcaca gaagcagggc atgatgaaaa tcgtcggtgt aaacactgac
    22621 ctttcagcat ggattttaga caaaccggaa acgggaagaa aattcccgaa aacgggaaaa
    22681 tccttcccga aatcaggaat aaccttcccg aaaacggtag acacccaata caagaacaag
    22741 aacagtatta aaagatcttc gtccgagaat tctgacgaat cctctgacgc acgtctgaag
    22801 aaatttttat caactcatcc tgaagctgcg gtctacacac catccggtgc gaagtggggc
    22861 tctgctgaag acctcgagac agctaagtgg atttcctcca gggtgaagct gattaaccca
    22921 acctgcaaag ccccggacat gacctcctgg tctaacactg ttcgcctgat gcgccagata
    22981 gacaaccggt cgcaccagga catctgcgcg ctgtatgact gggctagcaa acaccacttc
    23041 tggcagacca acatcctgag tcccgaaagc ctgcgtaagc agtgggacaa gctgacaatg
    23101 cagcgtaacg ccggaggtga gcagcgcgct gtcaagccag atctggactt caacaacact
    23161 gactgggcct atggggtgat ccgatgaaat ctcttgcaga gcagatgcgt aaccacgacc
    23221 gcgagcagat gagccgcatg gcccataacc tgccagagca gtaccaggag tgcgcgccgg
    23281 tcgagcaggt ggcgcaggta ttcaacaagc tgttcaacga gctgcgcgcc gcgttcccgg
    23341 ccagcatggc gaacttccgc acccaggaag acctgaacga attccgccgt cagtggctgc
    23401 tggcgtttca ggagaacggg atccacacca tggctcaggt cgatgccggc atgcgcattg
    23461 cccgccgcca ggagcgccca ttcctgccgt cgccgggcca gttcgtcgcc tggtgcaagc
    23521 agagcggcgg cgcgctgggc gtcaacgttg accaggtgat cgccgaatac tgggactggc
    23581 gtaaccgctc gttcgaattc atctccagcg agcaatttcc atggtcgcag ccggtcatgt
    23641 accacatttg cgtagaattg cgccaccgca gcaccgagcg ccagttaacg catggtgaac
    23701 tggcacgcga ggcaggcgat ctgctggaca tgtgggaaag gcgcgtcacc gagggtaagc
    23761 cagtgccgcc ggtacgccgg gctattgccg caccagctgc cgagcaaggg ccgacgccga
    23821 tccagctgct gctggccaag tacaaccgca acaagtcgaa cgggatggtg tgacatgaac
    23881 ataacaatcc gtgagcaggt gctggcagcc ctgcgcaaca acccagggtt gaacaacgcc
    23941 aaactggcag ggcttatcgg catggacacc aaaaagatat ccggaacggt gagcacgctg
    24001 ctggcagacg gcctgatcga gttcgaaggc aagcacggac agcggcttta tcggctgacc
    24061 gattacggca tgaaatacgc accagaaaca atcccggcta tgccgaaggg aaattcgaag
    24121 ctggtgcagc gtacagaggc aaacgtgatc tgccaggagt gccgcaacag cgcggcgatg
    24181 agaagggtat tgatggtttg ggggagggta ggggtatgag caacatcgac aaacgcgcat
    24241 tacgtgaagc agccaatgcg gcaaacgctg catcatgggg gaattgggag ccttacaagc
    24301 cacacaaagg tgcgcgcgga tatgaggtga aggttggtgt gaaagcggta gcacaacatt
    24361 gcctcaaggt cgattcagta ttcatcgccg cagccaaccc cgtcaccgtg ctggcgctgc
    24421 tggcatcgct ggaagccgca gagaagcgaa tcgctgaact ggaagcgaag ctcgatagcg
    24481 cagataaatt gcaagatagc gcatttcgtc atggtcttca gcatggcttc agttttggtc
    24541 aaacggataa tcaggctgga tttgaagagt gcttatctgc atatggagcg cggggtaaag
    24601 ataatggcta attcattact tgaaacatgc aatgactggc aaattcttcg cgcagaaata
    24661 ttggctcgaa atccaaatat ggccatgaca cttcaaaagc tcgacatcat gattgagcat
    24721 tccgttaggg cggcaattga aatatcgcat cgtgttgact gggattttag tgaagcagag
    24781 cgtaaagcta aggcggtgaa ttaatggaac tttcacggga taagattaaa gaaataatca
    24841 atgacgattg gcttttgatg gatgattgcg aaggaaataa aaactgtgac gttgtcaaag
    24901 agatggcgcg tatagcgctg gcatcgctcg aagcggagcc ggtggcgtgg ttgctgtcag
    24961 gaggcggggc aaaaaacaac gtcagcttcg atagtggcaa tgcttatgcc gacccgctgc
    25021 gagaagtaac gccgctttac accgccccgc cagcgccggt atctgtgccc gctgcgatgg
    25081 aaattgatga tgactttgac agcgcgtttg aacacggaaa agctgtcggc tggaacgcct
    25141 atcgcgccgc catgcttcag gccgaacctg taagtaatag tgatgagttg acagacggtt
    25201 gggtggcttg cagtaagcgg atgccaagcc catcaacagg tgtgatagtt gctgcgccat
    25261 ggcaatcagc tcctggcggc tatgcaatga agtgggcaac aaattgccct gggcatccgg
    25321 atgcagatat agatggctgg attataccag gagcatcatg gagcccaacc cactggatgc
    25381 cgctgccggc agcgccgcag caggaagata ttatttgctg atctaagatg gtctgtatgt
    25441 aacaaccacc cctaacttac tgcctaccgc attgagagaa ttagaaatag ttgtataggt
    25501 ttctttgggg gtggtgtttc ttccttcttc taaaatagac gcaataagat tcagattcca
    25561 cgaggcctta aggacatctt tgtatcgagg aaggagacgt aaagtagcca acattccata
    25621 caagggtatg gcattagatt ttgacaacag caaagctgac gcctgtttga tttcgtttat
    25681 tacttcgctt ttcgcattga tgctaaggat tttattttgc tcacgcagga gaatggccga
    25741 aaccatcccc aaatgttcct tgaatgaaat gtaaggatca agcatggatt ttacaaatat
    25801 ctgacccaac acaaacactg aaacaccagt aatgacggta gtaaacaccc cattattcat
    25861 aaccaggcca tcccttctgt tttttatccc gatatcataa tctacggatt gtttaacaag
    25921 ctagatcaaa tccagccttc cctgaagtac taattgcaaa tgtattttgt gaatcatccc
    25981 ggcgggtaga gatttataaa ccagcatcaa gacaccaatg gcctcttcga aggcctttct
    26041 cttatgttga ttttccataa tcaaccagcc ataatttaac cgtcgtcgga gttgaacgcc
    26101 cgacggtaag acttctgcgc atttaagggg acttaaatgc gaccacaatc tgaactcatc
    26161 gccttgtcac agatgcagac atgcacctgc gattttctgc attctgcggt ttccgtcaag
    26221 gaggccgtat gattatctcc aaagacggca tcaagctaca ccgtggaaat cttggtgcta
    26281 tcactcagca tctgaagcca cttctcgaaa acggtgagtg cttccggctc caactcaaag
    26341 actggcgcga gaagagaagc ctttcacaaa atagtctgag ccacgtttgg tacaaggaaa
    26401 taagcgacta cctgatcaag tctgggcgca ctgacgcaac gcctgcatgg gtaaagcgaa
    26461 acctcaaaaa aacttatctg ggttatgaag aggttgagta caccgatttc gtcaccggaa
    26521 ttaagacgat tgaattagaa ctccgccaca cgtccgatct ggacactggc gacatgcacc
    26581 atttcatgtg ccaggtggaa ggctggtgcg ctcagtttgg cctggtgctc acaatccctc
    26641 aaagcagcga atttcaggtg ctgcgcgata agcaggaggc ctgatgtcaa ctccactttc
    26701 ccgcgtcatc acaaacgaaa tctttcgcgt tccggcgcgc cgcaagccta agcccgcggt
    26761 taagccgtcc gatatcccga ccctgaaaga ctacaccgcc cgcctggtgg atcagaaatg
    26821 gctgcgtctc gcagcgagga gaaaatcagc atgagcatgt atcaacgaat taatggcgct
    26881 gactggcgca atatcttcgt cgtcggcgat ctgcatgggt gctacacgct gctgatgaat
    26941 gagctcgaaa aggtttcgtt cgaccctgcg cgtgatttgc tgatctcggt tggtgacctt
    27001 gttgaccgcg gcgcggaaaa cgtcgagtgc ctggatctga ttactatgcc gtggttcagg
    27061 gcagtgcgcg gtaaccatga gcagatgatg gttgatgggc tttcagagca tggaaacgtc
    27121 aatcactggc tggtaaacgg tggcggttgg ttcttcaatc tcgactatga caaagaggtg
    27181 ctggctaagg ctctggttca caaggcagct gagttaccac tcatcatcga gctggttacc
    27241 tccgatcgga aaatcgtaat ttgccacgct gactacccgc ataacgaata tgcgttcgac
    27301 aagccggtcc cgaaagacat ggtcatctgg aatcgtgagc gggttagcga cgctcagggc
    27361 ggcattgtct cgccgatagc cggtgctgat ctgtttatct tcggccacac ccctgcgcgc
    27421 cagcccctga agtatgccaa ccagatgtac atcgacacag gagcggtgtt ttgcggaaac
    27481 ctcacgctgg ttcaggtgca aggtggtgac catgagtaaa acctaccgca gcaagaagtg
    27541 gctcgccgca gtcggccaga ttgagcaatg cgtcctttgc ggagcgtggg gcgtacaggt
    27601 agcacaccgc aatgaaggta agggaattgg catgaagaca gacgactgcg ccaccgccgc
    27661 tatctgtgtc acctgccatt cagagattga taacgggaag gggcttagcc gtgacgagcg
    27721 ccgccagtta atggatcgcg ccatcgtcct gaccattatt cagattgccc gtcgtggctt
    27781 ggtggtgcct gcatgaaaat ctacgacatc acaccaatcg gcaagcctcg catgacccgc
    27841 gcggataagt ggaagcagcg tccacctgta atgcgttacc gcgctttttg cgatgaggtc
    27901 cgcctgcgca agttgaccat gcctgaatcc ggatcacatg tgacattcgt cctaccaatg
    27961 ccaccaagct ggagtaagaa gaaacgagcg gagttcgccg ggaagcctca ccaggccaag
    28021 ccagactgcg acaacatgct gaaagccctg atggatgcgc tttatgagga tgatgctcac
    28081 atctgggatt gccgcatcac aaaggtctgg ggagagaagg ggcagatcat tatcggggag
    28141 tgcgcaccgt gaccagagac gagataaccc gatatcaggc cgaaagcgtt aagcgcgcca
    28201 acctgccgcc aatagcaaag cacagccaga ccaaaaccaa ccagccacag aaggaagccg
    28261 catgaacagt cagcaactgg aatacgtacg tcagcagctc attgtggcga ccgcagacct
    28321 cagcggggcg acgaaagggc agctggtagc tttcgccgag aacgcgcaat tcaccgcgac
    28381 ggcgcggagt cggggccgga aaaaggtatt cgacaaggat aagcagcgca tggtcaatcc
    28441 tgacggccag ccgatgagcg gcaaccagtc ccgcgctaag ggctcgtcta tcgcgctggt
    28501 gggcccggta gagtacggga ccgcatcgtg gcgccgcgct gttctgtcgc ttgaagacca
    28561 tcagaaagcg tggctgctat ggaactatag cgagaacgtg cgctggtgct atcaggtgga
    28621 aatcactcaa tgggcgtggg cagagttccg ggagcagctc ggcgcgaaga aggtggccgg
    28681 caaaacgatg gagcgcctga agaagcttgt ctggctcgcg gctcaggacg tgaaagcaga
    28741 gctggcaggg cgtgagacgt atgaatacca aaagcttgcc gccatggtcg gagtgacccc
    28801 gaagaactgg tcagaaacgt ttacagaacg gtgggaggag atgaaaacca ccttgcggcg
    28861 ccttgatagc gattctcttt tgcaggtaac gcgatcacgt tcacaacaaa aggcgacaaa
    28921 tttagactca agtcttgcaa aactggatta aatgcgtcat atttgagtct actttgatat
    28981 gctgccttaa ctttaagtgg cggcatgaag tttgaaaaaa gttggtcggc aagccatcag
    29041 taaaaagcag ttagacagcg gcagtcttta aaagcaacgt aacggctcga aagtgagcaa
    29101 aaatacaagc ccgaggttaa cgccttgggc tttgtcgttt ctgcaatctc agcgctgttt
    29161 aaaggtttcg tacccacagc tggagcattt gtaatagtct ctctgaactc ccagcccaat
    29221 gaaatcgggg tcatctacgg ttcggtcaag gctatagctc atctgtgagc atctggggca
    29281 tttctcccca ccgctggatt tgatgtatgc ctcaagctcg gcgacctgct gtttgagacg
    29341 atccacttcg tcgggtactg tttttaacct tttccacagt gggatttttt ccagtaaaga
    29401 atccagttcg gagagtatcc ccatgagtca cctcgttaat aagacgctag ttacgacagg
    29461 cggcattacg ctatgcaagc ctgatattta tgcccttgat atcactactg atgataaagg
    29521 gagtaaagaa ctctacattc tcaagaatgg cgaagaattg atccacttcg aacttacaga
    29581 agagagtacg gaaaagttgt tatccttgct ccgagagtag gtctatttgc attgcatcaa
    29641 tacccctgtc acatcgtcgt agagcattga aacgagtttc atcagatgtt aaatttttag
    29701 tgtggtgaat ccccctatgc ggaggggcga ccagtcaaac ttttttgtcc aggtttgttt
    29761 gcgcggaact agtcggctgg ggctgttcca ccgggaggca cccggcgcca cctccttgac
    29821 ggtattgtta tttttcatgc ctgttcgtcc gagcaggctt tttttgcccg cattatcttc
    29881 attgaaaact ggcaactcag agaatcatcc aaattaaaaa agcagcaacg agagcgatta
    29941 ctaatacatt cttcgctttt tctcttctgc tggccttcac aaaaggattc actatccggc
    30001 agtggctaca cacaagtgat tctgggttaa ttaactttcc gcagtagggg cagggcttga
    30061 taatcattgg tactaattaa gttgtgagaa cgtagtctca tttaaccaca tttatcgagt
    30121 tgttttacta atggtctagt tatttcagac atattcatca atttcaggct cacgggaatc
    30181 atccgctacg tgctttgttg ataaatccag cccgtgaagc ctgacccttt tcaaacacac
    30241 acacagcgcc atccgaaaaa tcggaggtga ggctatgacc agaatgagca ccatttacag
    30301 cagactttca tatggaacag gaaccacgct gaccggctgc ggtgtatcag cgaaggcata
    30361 tgccgaaaca gctaaaacag caaaagaggt gtcctggatg ttggccgaca gaattgcagg
    30421 gttaagcctg agcgactggg caattattgt cggtatcgca tgcactgtta ttacctgtgc
    30481 agtgaactgg tatttccgct ggaaagaacg ggaggatcgg cgcaatggct atgccaccaa
    30541 agctgaagaa tagcgttatt gcagcgatac ccgctggcgc tattgctatc gctgcggcgt
    30601 tgattactgg cccaacgggt aatgatggcc ttgaaggtgt acgctatcag ccttatcggg
    30661 atgttgttgg agtgtggact gtatgctggg gccatactgg taaagatatt atcctcggca
    30721 agacctacac caaagcagag tgccaggcgc tgctggataa agacctgaat attgtcgccc
    30781 gacagattaa cccatatatc aatgctccga tccccgaaac gatgagaggg gcgctgtact
    30841 catttgcgta taacgtaggc gcgggaaact tcaaaacctc cacactgtta cgaaaaatca
    30901 accagggtga ttcgaaaggc gcatgcgaac aactgcggcg ctggatctat gctggtgaca
    30961 aacagtggaa gggtttaatc acgaggcgtg agattgagcg tgaagtttgc ctgtgggcag
    31021 agaaacctca ggttctgggg gatggactcg ggccgcttaa ccctggcatt ccggtatcag
    31081 ttccgggggt attctgatga aacccagaaa aattacgatt gttgcggttc tgctggtggc
    31141 tgtcgtaatc attattgcag tgcttagcgt attactggtt cgtagccgct cagctcttga
    31201 aacagaacag agtgagaatc gggtattacg taatgataac gcgctgcagg cgacggtgat
    31261 aactacacag gctttcaact tcaatcggtt taaccagata gcagagaacg ccaaccgcct
    31321 taactcgctg atcgatgccg gtactgagaa aactgtcatc gaataccggg agattctccg
    31381 acgtgaaaag acctgtgatc tgcctgttcc tgctgatgtc gctggtgggc tgctcaaata
    31441 cgcgtaccgt ttacgtgcca gcgcaatgca ccccgatacc gggaacacta acgcaaccga
    31501 tgatagtacc gctgccgcca gctcaatgac gtattgccag gccgtcctct ggattgaacc
    31561 tttgctggct gttattgaga agggcaacaa taacctggcg ggtatacgag agattgagaa
    31621 gttacgaaaa tgaataatgt ccgttgacat tgttagggtg attctcattt aacaacctca
    31681 actgctacat tacagtgctt catcatgcaa ttagcttttt caccaatggc ttagatccag
    31741 tgaaagttac tgacgttatc taatgacaag atgaacgtag cttttgtgta gtttttcagc
    31801 gaataggatt atctgaattt agcatttttg gcttgtataa actgtgggta agtagctata
    31861 gtcacgtcat agatagtatt tttgcatctt tttcaagcct cttttgaggc tttttgcaca
    31921 tttaaatatg acaacaatgg cacatatagt atgtccccct tggcacaggc actatatgta
    31981 gcgctataaa aggagctatc attatgaccc cagctgagtt ctacgatgtt tacaatatca
    32041 aaccggcaga aatgttgact ggtgaaactg ttaacaactt cgcttctcgt gtaatggcac
    32101 agcaaactag tacgagtgga aacacaggtg tttggtactc acaaggtacc gctacacaat
    32161 cgaagcaaaa ccaaactaca agtcatcaac tttacacgta ctgatttatg cctaattgga
    32221 gcgacgtact gggcgagata acggctctcg cccataaaag tcctatggat gaggttcgcc
    32281 gtaaatactt atctcaactt tcgaatcata ctggaagaaa tgtaatcaca tattactcag
    32341 ggtggttaca gcatggtggt gcagaagtac gccatcttac tcagatgact gatgatgata
    32401 agaacgggtt gatgacagcc atcaatggtt tagatgtatc aaaagggttg gatttgatac
    32461 ttcatactcc aggtggtgat attgccgctt tggaatcaat cgggcattac ttaaggtcga
    32521 agtttggaac taacatcaga gcaattgttc ctatgatttc aatgtcctgc ggaactatgc
    32581 ttgcatgttg tgccaatgaa atcatcatgg gtaaacagtc caacattggc ccaattgatc
    32641 ctcagttcaa cggtttctcc acccatgcca tcattgaaga atggaatcgt gcgcagacgg
    32701 aaatttttca aaatcccgca gctgttcaga tgtggcagtt cattcttcaa aagctaaacc
    32761 cgacgatcat cggcgagtgt gagaaagcaa tcaaatgggc aaatgagatt gttaagcatt
    32821 ggcttatgac aggcatgttt gataatgatc ctgaggcaga atcaaaagca acacatgttt
    32881 gttcagagtt aaacaaccat cacacaacct atacccactc gcgtcatatt cattttgata
    32941 aggcgcagaa aattgggttg aatgttaccg aacttgaaag tgatcaagta cttcaagatt
    33001 tggttttgac tatacatcac agctacatgc attcttttgg tggagcacca ctggcaaaaa
    33061 tcattgaaaa tcataacggt aacgcaatga tttggaatat ccagtcttaa tcccccctgt
    33121 ctgattccag cctcgcctat gcggggcttt tttataccag cagtaaaccg cgcatcgcag
    33181 cgcatgacaa tcccgagtct ttcagaaagc tgagcctgag aactgccgta tatggtggcg
    33241 accatctcgg ggcggctttt ctgtgcgaac aggctcatct ttctaaaagg taaaaacgct
    33301 atgaataatc cgtcagttat tccggccttc gacttccgcg aaatggtgca agccaaaaac
    33361 ggagaggtcg tcaccacttc cagaaaagtt gccaagtact tcggcaagcg acacggcgat
    33421 gtgctcagga aaatcgagca ggttaagtcc gattgctcga gtgagtttgg ccaacgcaat
    33481 tttgcgtcgg ctgattatat cgatgagcag ggtaaggttc gcccgatgta cagcctgaca
    33541 agagatggct ggatcatggt tgtgatggga ttcactggga gagctgctgc agcaatcaaa
    33601 gagagttata tctcagcatt caactggatg tccgaacaac ttagccgacg catggcgatg
    33661 ggtgaagaaa tgcagcaccg ctatgccatc aaagaaacac gctcgaagct gaaaggcacg
    33721 atcggcagtc gtttaatgaa cgaacgcaag aaggaaaagc gcgtcctggc actggagcat
    33781 gagcacatca tgcaggtgac gcagccagaa ttgttgattg gctgatcgcg acattacagc
    33841 aggcattccc tgagtgtctg cgataatgct ttattatcat tgttaacgac ggggtaaaaa
    33901 tcatgaaaat tttagttgaa cacaactcca aagttatctg gatgcgtgat aatgaaacat
    33961 ctgaaggtgt ggcctgcagg agttatatta aggacggcgt acagcaaaag attattgccg
    34021 cccttgagga tgccttagct caggcaaagg gtgagttatt atgctggaat gactctgatg
    34081 ctgtgagtga catttcctga gccaccacct aaatcaatcg taacgttcca gtatcccata
    34141 tgaggcgcaa ccaaccgggt cggaagtctc tcgaagaaac caccaccacc atgatgatga
    34201 aaaccccggt tatttttata actattgaag ttactgtcgg tagtgagaag gatgttgcac
    34261 tggtgcgagc agtcaacaac aacgacatca cctgcattca ggtgcattcg tttgtgaaga
    34321 aattgcataa gtctccttat caagaggcaa tcagcactcc tctcaaggct ggtacgccaa
    34381 cgtcccatcg ctgacgggct gagccattac cttacccaga ctcaacgata agtaacatcc
    34441 tgatattcag acagtaactg taatcgataa tgctgaagaa taggcagtaa atacaggtga
    34501 ctgatatggc aacactgaag gatttatcaa accagttaaa gcaactgcaa aagcaaatcc
    34561 cgtttgccac agctcaggcg atgacaaaag ttgtacgcca gattgagttg gctcagaaga
    34621 cggcattcga acgacatctg gagagcccca cgccgtttac cgttaaatca gttggttcgg
    34681 tggcggcaag aaagaacaat ctgaccgcaa aggtttttgt ccgcgatacc gctgctggtt
    34741 atctggaacc attcgagttt ggcggagagc acaagctcaa tagccaggct ctattaaatc
    34801 ccaagaacgt taagctaaac aaatacggca acatgccgcg taataagctc tcacagctta
    34861 aaacaaagga aaatgtattc gtaggtgagg ttgatggcgt taacgctgtc tggcagcgta
    34921 agaagccgat gaaagctaag aagcgacggg ccaaacgttc cgctaacggg acgcgaagac
    34981 cgaaacgcaa acagcgttct ccaaagcttt tgatccggtt tggtgatgcg ctacctgtga
    35041 ctccagtgct ggggtatatg gataggtccc ataccatggc gaacgcactg ctaccgtctg
    35101 ctttaaatca ggcgatagca gaagccatca ggacggcaaa ataaaagcag taacttataa
    35161 gttaatttcg caagctttta tgaagctgtt tactgcagtt gtcgatccag aaacattggc
    35221 tgacatggaa tgctggtttc cgccatcctt tgtttgcaca ccaactaaca ctttggattt
    35281 cgccccctga agctgcttaa gcactgtttt tagttggtcc gcgtcatccg attgaatctg
    35341 gaggctctga acattacgtc ttgaaagggt agcatcgagc ttcactgcgg tattcccgtc
    35401 gaccttcatt atcaggtcca ttggtacctc tgatagtgat tcggtgcttt tatccatttc
    35461 aacgtatgcc gccgatagct tttctttagt gcagtcaaac acaatggcgc cattgtcgga
    35521 tgaaacctcg ccaagcatca ttgctttctt accaccagag aaaaggtcat cttcagtatt
    35581 agttacccac tgggcatgag caattggtga tgccagcact gcggctacga aagttatttt
    35641 gattatattg ttacccatta cattctcctt gtattgaata ggaataatca tagtcggagc
    35701 gaatggtcga agccataaaa aaatgggtcc ttcctgagac ttttgtaagg tacgggcatt
    35761 gcgcgccgcg gtgttttcct agctacaact ttcagatttg tgtcccatgt cccacctctg
    35821 gcgatcatta cggacacctc gccagctctg gctattccag tttattccag tgggacattc
    35881 tggtgggaca tggcaaaaat gtcccaggcg aatgtcccac cccagaaaat gtcccaggtg
    35941 atgtcccatg accacgatga accagagtca gtacgcacaa cattcaggtg tggatcgcaa
    36001 aacaattggt cggtggatta aagccgggcg cttcattgtg atggacggag acctgattga
    36061 cgtagaggcc agcgatgcgg cattgaagaa aaaccgcgat ggcaaagacc cgcgcgcctc
    36121 gaacgcgaag aaaaagaaaa ctcccgtcgt tagcgataac gatgatgacg gtgatgaaat
    36181 caataaaact gtccgccaga taatgctcac tgaaggggca gatctttcga gagaggaagc
    36241 gggacgtatc cgcgagaatt acatggccct gcaggcaaag ctgcagtatg aaaaagacag
    36301 cggccagctt attgagctga cagcagccga ggaggtttta ttcaacgcct ttcgccaaca
    36361 gcgtgatgcc tggcttaact ggccgtccag ggtggcgccg ctaatggctg ctgatctgga
    36421 tgtaccggcg gacaggatga cagaggtgct gattgaacat gtccacaaac acatctcagt
    36481 cctcggagag ccagagttta acccggcaga agattgagcg tcttgaatta agcgtccgca
    36541 aaggctggac acccccgccg cgtatcagtg tgccgcagtg ggcagatgac tatcgtaagc
    36601 tggcaaaaga ggctgggagc acttcgggaa actgggaaac atcgacggta gaaattgccc
    36661 gcggaccgat gcttgccgcg acggagtccg gggttcatat catcactgta atgtgctgta
    36721 cccagttgat gaagacagca ctgctggaaa acctttttgg ctattttgcc cacctcgatc
    36781 cttgtccgat actgctgctg cagccgaaag aagaagccgc tgaacagttt tcgaaagagc
    36841 gtattagccc gctggtaagg gtgacgccgg tactgcgtaa aatcatcggt gattcgaaac
    36901 agaaaagctc gaaagaaacc attctttaca aggcattcac tggcggattt ctggcgctgg
    36961 cgggtgctgg tagccctgat aaccttgcgc gtcgtccgat tcgtgtcctg ctggcggatg
    37021 aagtggacaa gtacccgata acccgcgaag gcgatccaat tgcgctggcc gaagagcgta
    37081 cagcgacatt tggcctgacc tggctgtctg tacgcgcctg ttcgccgacg gtggaggatg
    37141 agagccgcat tgctgacagc tacgccgact ccgatcagcg ccgggcatct gtggtttgcc
    37201 cgcactgtgg ccaccgccag ttccccgact ttttcaaaca cgttcagtgg ccgaaagagg
    37261 gagataaaca cctgactaaa tcggcgatgc tctattgcga atgctgtggt agtggctggt
    37321 ccgaaggaca gcgcctcaga gctctgcaca ctattcgatg gcatcagacg cgcccatttg
    37381 agtgctgcgg ggagcggcac tcaccgctga tggattatga ccttgcctgg cgggcggcag
    37441 acgagggcag cgttgaaaag gtctggcaat ggtcagagtc ggaacggcat gcggtctatc
    37501 gcgcaatctg cccctcctgt ggaaaggagg cagtcgataa ccaccacgcg gggtaccagg
    37561 catccaagct tttcagcccc tggcaaaaag ataagccgtc ggatattgcg aaaaaatata
    37621 tcgatgcgaa gggcgatccg gataaggaac aggcgtggtg gaatacccag atggggcttc
    37681 cgcaccgacc taatcatggg aaacagctcc ctgttgatgt tctgctggcg cgccgggaaa
    37741 tatttccggc cgtcgttccg gacggggtgg cattgttaac agctggagtt gatacccagg
    37801 acgatcgctt cgaaattacg atcaccggct gggggagaga tgaagaatcg tggtcggtcg
    37861 cgcatgacgt tatttatggt gaccttgaga cggaagaacc ctggaagcga ctggatgcat
    37921 acctgaaaca gatctggcga cgtggtgacg ggcgcggcct gaatatcatg gcaacgtgca
    37981 tggactccgg tggccaccat acgcagaagg tatacgaatt cgccaaagag cgtcttggcc
    38041 gtcgtgtctg ggcaattaag ggggagtctg cacagggagg taaacgcaat cctgtctggc
    38101 cgaccaaacg accatcatcg aaaagcaaag ccagtttccg ccctgtcatt ctgggggtta
    38161 actcagcgaa agacgtgata cgcggtcgcc tgcatcttga gccacccaaa cctggcgccg
    38221 ccgctgcggg ttatatgcat tttcctgacg atcgcgatct cgggtacttc aatcagctgc
    38281 tggcggagcg actggtttac aaagtcattt ccgggcagcg gtacagtatc tgggaagcaa
    38341 taccaggacg agctaacgaa gcgcttgact gcctcgttta cagctatgcc gcgctgtgcg
    38401 gtctcaaaca tatggggtta aaactcaacg tccgggccgc caacctcgaa gccgatccgg
    38461 ataagttcct gccagcgcca gttggacagg aagaaaaaat caattacgag ctgccgggtg
    38521 cggttattga agaaccagcg ccggtcaaac gtaagcgaat atcgcaactc ctgccgaaat
    38581 aaggaaaatc atgttcaacc ggaacaccag cctgcttgcc ggcgcaatga ctgacgatca
    38641 gctcagggat gcgcttgcga aagctcagca ggcgtacatt gatttagcaa ccgggagcca
    38701 cggtgtttcg ttttcctata cgcagggaga cgggacgcga tcagtgtcct atcagcaaag
    38761 caccctggct gatctgctgg ccctgattca acttctgcag gcgcaactgg ggattatctc
    38821 tcgtccccgg aaaccagcga ggtttagatt ctgatgaata aagtacagat actgggctct
    38881 gatgggcagc cgttgcgaca gcagcgtccc tctatgctgg tgggggggag ccgcgtacct
    38941 tatgacgcag ctgactcttt cagcgatcaa ctggcgaact ggcaacccgc gctgtggtcc
    39001 ccggacaatg aaattaacat ttaccgggat cgcatcgtgt cccgcgcacg cgatctggtc
    39061 cgtaatgacg gctgggcaaa cggtgcggtc acacgtctgc tggataatgc ggttggtgcc
    39121 aacttccgcc ccatcatgaa acccgattac cgtgttctca gaatgatcac cggaaacaag
    39181 gcgtttgatg cgtcctgggc ggaagagtac ggaaaagcac tggacgggca ctggcggacc
    39241 tggagtaacg atcctggccg gtattgtgat gttgaacgaa aactcaccgt gtcgcagatg
    39301 ttacgcctgg gatttcgtca taagcttatt gacggggatg ctctggccat tctccagtac
    39361 agaactgaca ggcttggtcc cggaagaggg cgttacgcca ccacggtaca gattgtcgat
    39421 cctgaccgcc tcagtaatcc tcagcagaat ttcgatatgc caaatgtccg tggtggcgtt
    39481 gaaattgatg cggacggtgc gccggttgct taccacatca gggaggccca tatcggtgac
    39541 tggtggagcg gggctaaaac catgacgtgg cagcgtatcc cgcgtgaaac tgactggggc
    39601 cgcccgcatg tggttcacga ttttgatcat gagcgtggcg cgcagcaccg cggtaacggc
    39661 atcctgactc cggttattca gcgtctgaaa atgctggtga agtatgacca gagtgagctt
    39721 gaggcagcaa ttcttaatgc catatttgcc gcttacattg agtcacccta tgaccctgcg
    39781 atggttcagt ctgccctggg cgagacctat gacgagtcgg agttaggcac ttatcaggac
    39841 gggcgtgttg agttccataa cgatcggcgt ctgacacttc agaatggtgc ccgaatgccc
    39901 attctttatc ctggtgagaa aatcacgacg gttaacgcgg cgcggcccta cagcaatttt
    39961 gaagtcttcg aatctgctgt tctccgtaat ttttcttcag gaacagggtt gtccccacag
    40021 caggtcaccc aggactggtc tgacgttaac tacagttctg cacgctcctc gttgctggag
    40081 gcatggaaaa cactgactcg ccgccgggac gatttttcta ccggcttcgc tcagcccatt
    40141 ctcaccgcct ttgttgaaga agttcacgac aatgaggatt tacccctgcc cgcaggcgca
    40201 cctgattttg ttgacgccag agccgcgtat tctcgcgcgc gctggatggg gccagggcgc
    40261 ggctgggtgg atccggttgc agagaaaaaa ggcgccattc ttggtctgga tgccggactt
    40321 tccaccctcg agattgaggt gggtgaaaac gtcggtgaag actgggaaga agtgcttgat
    40381 cagcgccaga gagaaattga gtcatgtctt aaacgcggat taccgcttcc gagctgggca
    40441 caggctgacc agtttgcgag ccagaccatt accgaaccgg aggaaaagtg aatctacccc
    40501 atctggccca gcgattattt aacaccccgc tggcgctgca cccgagtaaa gccgaagtca
    40561 tcatggcatc cgtaatggac cgatttggta tcagtaaaat cgaatcttct cttgccatgg
    40621 aggatgactg gtacggatat gacgataacc ggggacgtga atcccgtagt gatccgggtt
    40681 atgacaatgt gctgggtgtc gccgtcatcc cgatatgcgg aacgctggtg caaaaactgg
    40741 gcagtctgcg tccgtacagt ggaatgacag ggtatgacgg cattcgtcag gcgtttctta
    40801 ctgcgatgga agatcccgac atttcgggaa tttgcctgga tatcgactca cccggcggcg
    40861 aggtcgctgg atgcttcgat ctggttgatg tcatttacgg ctcccggggg aaaaagccta
    40921 tccatgccat tctgacggaa agcgcttatt ccgctgcgta tgccattgcc agtgcagcgg
    40981 accggatttc tgttccgcgc accggcggag tgggttctgt gggtgtgatc accatgcacc
    41041 ttgactggac gcagcggatt aaagatgacg gtcttaaagt tacgatcatc acctatggat
    41101 cccgcaaggc tgaaggttcg ccgctgagag agttgtcaga tgaagcgctg gccgccatcc
    41161 agcaggacat taacaccatg ggcgaattgt ttgtgaacac tgttgccaga aaccggggga
    41221 ttagcgcaaa ggttataaaa agtacccagg ccgcctgttt tatggctgct gatggcgttg
    41281 aaattggact ggctgatgag gtgtgtcctc ctgacgctgc gttcaaaaac ttacttgaaa
    41341 aaacaggagc ctgaaatggc aaagaaaaag acgtttagtt ttgctcacct cattggtctt
    41401 ggcccttccg cttctgagga agaagaggat aaaaaagcca aaaaagcgaa agcccgtcgc
    41461 gcggaaaagg acgagcgcga agatgatgcc gatgatgatg agcgcgacga cgacgcggaa
    41521 gaagacgaac gcgacgatga tgctgaagat gacggcgatg atccggatgc gtcagaagat
    41581 gatgattctg aagacgacgg cgacgacgat cgcaaagaga gtaaggcggt aaaaaatgca
    41641 cgcgctgctg agcgtaaacg ctgcgcccgt attttcggca gtaagcatgc agctgcgaat
    41701 ccttcactgg ccgcgtcact ggctttcaat accgggatga gttctgcggc agcaattaat
    41761 gtcctagcct cttcggctcc ggccgcagcc gcatctcagc catcccgtaa acgctctctc
    41821 gatcagcgta tgcaggaaag ccaccaggtc cggcttaatc cggatagcgg acagaaagag
    41881 accggaaagt ctgcgctggt aagtaaaatg accggcctct acaactccac aagaggagag
    41941 aaataatgga tcagtttggt cagaatgcgt ttgcgcctgg catgaagagc gcgctgtttg
    42001 ttccggatca gcttgtcgct ggcacgctcc agctggtgac tgacaccggg atcattacgg
    42061 gcggtgcctt taagcgtggt actgtcctgg gcctggtggc tgccagcggg aaatacacgc
    42121 aatgtgtgaa aacggctgaa gatggcagtc agttacccgt tgctattctg gttgatgatg
    42181 ttgatgcatc gtcttccgat cagaacggcg gcctgtatct gatgggggaa ttcaaccagc
    42241 accgaattat ttttgataac tcctggacga ccgctgacct gaaaaaagcg ctccgaccgc
    42301 tggctatctt cctgaaagac agtgaccagg cacctgtaac cacctcctga tttcccccac
    42361 ggctctcctg acgaatgctt taaccggcag gggctggctc gtttaaattt tttgccagct
    42421 gcggctggca ctatcaagag actgaatatg gaaaatattt ttgataccag cgtgctggtg
    42481 caggttgttc ctaacctgaa aaccagtcag aactggctgc tcgatcgctt cttcccgaat
    42541 gtcgtgactt acgagactga agaagtggcg attgatgttg atgtcggcct gcgtcgtatg
    42601 gcgccgttcg tctccccgct ggtggaaggt aagctggtcg aatcccgtaa ataccagacc
    42661 aataccttca aaccggcata catcaaagat aagcgcgcgc cggacctgcg caaacctatc
    42721 cgccgccaga ttggtgagcg tattggcggg gaatataccg ctgccgagcg cgaaatgctg
    42781 aaccttcagt ttgaaatgac tgaccagatt gacatgatca accgtcgtct ggaatggatg
    42841 gcggcgagtg cgctggtgtc tgggaccgta accgtcgccg gggagggcta tgaaactcag
    42901 gtggtggatt tcgggcgtgc ttcggatctg accatcactc ttagcggctc ggataaatgg
    42961 ccactgaccg ttgcagctgg cgctaccaat acccagccat cagatgacat tgaaatctgg
    43021 cagactactt tcctgaaaga gtccggctct gtcgccacgg atctggtctt tacgaataag
    43081 tcatggcgtg cattccgact ggataccacc atcaaggata acgccattac attcccggcg
    43141 ctgagcccgt ttggtaacca gattaacgcc ggcccacagg taatgaaggg cgcaatttat
    43201 aaagggcgct ggggtaactt tgacctctgg ttatataacg actggtttat tgacccgctg
    43261 gacaacgtcg agaagcctat gatccccgat ggcgctgtca ttatgagtgg tgccgatctg
    43321 atgggtaccc gcgcatttgg cgttatcctg gacccggctt tcaactacgg tccgctggct
    43381 tatgcgccaa aatcctgggt gaaagaagat ccagcccagc gtcttatcct gatgcaatcc
    43441 tccccgctgg ttattccgag ccgggtaaat gcatccctct gcgcaacggt ggtctgatat
    43501 ggcaaaacaa cctaataccg ggctggctga tgatctgaat gcagaaggat ctgccaaaga
    43561 cggcctgagc gttgacgacc tgaatgctgg cgataacacc caggaaaaac agcctttgag
    43621 caaaacagat gatgccgaat tgtctgttga tgacgatggt ggtgacgaaa aatccggaga
    43681 cactgaatcg caggagtatg tggtgttgaa agggaattgc attcgtcatg acggggagat
    43741 gtaccgcgaa aatatgcgca tccctgtaac cggcaaagat gctgagcgtc ttctgcagtc
    43801 cggcgttatt gctgatgttg atgtgcttcg taagcgagtt cttgcttctc agccatcagt
    43861 ttcagttacg acagggtaat gacatgggcg tggactggga ttctcatctt ctgagtccgc
    43921 tgcatgatgt ctttggcgat gagcacgagt accgtccacg taacggtact ccttttacaa
    43981 ttaacgggat ttttgaccgt ggttatgcgc aggttgctga aaaccttgat ggcgattcag
    44041 aaattaacac ctccagcccg atgttgggtg tgcgcgatgc tgaatttcgc aagctgggta
    44101 aatcgcaacc tgctgtatct gaccgggtat ttataaagac ggtcggtggt cacatcatca
    44161 atcagttatt tgttgtgtca aacgtcgaac ccgacagtca tggcggatct cgtcttgtcc
    44221 tcaatgtggt aaaactgcga tgaattcagc agcgatacgg caaatggttg tcactgcact
    44281 aaccgggaca accagcgcgg gcgaccgcgt attctctcca cgcgactggt caacttcacc
    44341 agatatgtat cctgtgttgt tggttcagac gccttttgaa cagaaaaaat cacaggggcg
    44401 taatacccct gcttttacca ccctcaccac tgtcaggatc actgggcgcg ttcaggagta
    44461 tgacggcgat acagtggatg atggagccat gcgggcagag ctggcgcttg aaagccttcg
    44521 cgagcaggtg gagcgcgcgg tgatcaacag ctacgaactg actcggaata ttcagaaata
    44581 cgcggaagtt cgttcaacca tcaatgttga ttcagaagga gaggcccata tggggcagct
    44641 tcttttcgag atcgacatag agcattacca ggggccggaa gatttttatc ctgtccagtc
    44701 ggttcccctt gaaggcatgg atattgcggt cgacatgcca gacggcacag ttaaaccggg
    44761 tatcagcctc aatcttcagg agtaatccat gtttgttaag ccgaacaacg ggctcagcgt
    44821 tcgctgcccc gtcaagggca tcccattgcc taaagagggt gctgaagtac ctgacaatat
    44881 tttctggcgt cgccgtctga gcgatgggga cgtgatcctc tctaaaaagg atgagggcgc
    44941 gccagagaaa caatcattac ctaaaaaagc gggagaaaat gaatgaccgt acctttcgct
    45001 cgtgttcccg ataacctgcg ggtagggctt ttcttcgttg agtttgataa ctcaatggcg
    45061 aataacgcca ctgccacgca gcgcaccctg cttatcggtg ggatgctcag taccggctca
    45121 accctccctg gtattccgca gcgagtttcc tcttcggata ccgtcggtga gctgacagga
    45181 aaagggggaa ttctgcaggc catgatggcg gcgtatcaga aaaatgatac cgcagccgaa
    45241 gtctggatcc tgccgctgga ggaagactcc gattccatgg tggctgcaac cggcaccatt
    45301 agagtgagca gcgcaccgac ggcaaccgga gtgatctccc tttatattgc tggtgagcgc
    45361 attcagttga ccgttgtagc aacagatacg gtggcagcga tcgccacctc tctggccgcg
    45421 gcgattaacg caaaaaccac gctacctgta accgccagtg cgactacgga taccgtaacc
    45481 ctgaccgcga agaatcttgg tgctacgggt aatgggatcg acattcgcct gaacttcctc
    45541 ggcttacctg gaggcgagtc cacacctgca ggcctggaac tgacgattac tgctatgtct
    45601 aacggagtcg gggctccgga tattaccggc gagctggcaa acctgcagga tcggacattc
    45661 gatttcatca tcaaccctta cgacgataca acctcgttga atgtgatgaa ggagttcctg
    45721 tcagacactg gcggtcgctg ggcatgggac aagcagcttt atggccattc ctttggtacc
    45781 accaccggga cttacgccca gctcggtacc aaaggtgagc tgcgcaataa ccagcatgag
    45841 accctgctgg gcgtaaataa atcgccgtcc ccttcctggg catggtctgc agcttacacc
    45901 ggcgcagctg cggtgagtct gcgtaatgac cccggccgcc cgctacagtc gctcgctgtt
    45961 cagggggtgc ttgcgccaga actgcaggat cgctttgagc tgaccgagcg taacaatctg
    46021 ctgtacagcg gcatttcgac atttacggtc gatgacgatg gcacggtgcg cattgaaaac
    46081 ctgatcacca cctaccagaa aaacagctat ggcgatgcag atgacagtta tctggaagtg
    46141 gagacgctgt tcagcctgat gtttgtgacc cgctacctgc gcacagcggt gaccagcaag
    46201 tttggccgta tgaagcttgc tgcggatgga acccgatttg cacctggcgc ggcgatcgtc
    46261 acgccaaaca ttatcaaggc cgatcagatt gccgagtacc agactctggt atggaacggt
    46321 tatgcgcagg atgcggaggc attcgcaaaa aatatcatcg tcgagcagaa cgccaaaaat
    46381 ccgaaccgcg tcgatgtgct gtggccggga accctcatga accagttgcg cattttcgcg
    46441 ctgctcaatc agttccgcac tcgggctgaa tcaacaggag cttaaacgat ggcaggtgat
    46501 actactaacc gcctggcggg aaccgcctat gtcactgtta acggtgtgac ggtaatggtg
    46561 gagggctcgt ttaaatacca ggctgccacc gtaaaccgta ccaccctgac agggatggat
    46621 ggtgtgcacg gatataagga aaaacctgtg gcgccataca tttctgcccg actgcgtgac
    46681 agtggcggaa cgaatgtgca gggctttaac cagcagacga acgtcaacgt gatcgccgag
    46741 ctggctaacg ggaaaactat cattggccgt tcactctgga cggtcaacgt ccaggaagtg
    46801 gaaagcgaag atgcagtatt tgatgttcgc tgggaaggcc gcgacgtaac ggagaactaa
    46861 gatggctgag attgaacgcg ttaaaaccat tccattaacc gtagcgctgg atgatgctgc
    46921 ggagaagacc acttatacgc agctggagct gaaagcaccc acgctaagcc aggctgagca
    46981 gttttacgag aaacaggctg cgtcaacgtc gctcgcggcg atgcgcctgc ttattgcgct
    47041 ggtttccggt acgcgtgaaa gcgtactgca gccgatggat tttctcgact tccgtaagtg
    47101 tgaggagtat ctgctcagtt ttttgacctg gaagccctga caacctggca ggaaatggcc
    47161 gctgacgtca ccttctattt ccgctggtct gaggacaggg cgtggggaat gacccgcgcc
    47221 cggctgaaat ggtgggtggc gcaggcatcc cggataaaca agcttaggaa acctgaagac
    47281 gatgagtaat tcttttgatt ttgagctggt ggccagcgac caggttagcg aggctataga
    47341 ccgcattaat gaggctgtcc gtgacctgga gccgaagcta gataaaacta aagaagggct
    47401 caagttaggc ggtcaggaaa cagccgacgg actgagcggt tttatttctc gcctcgagaa
    47461 tatgtcgaag agcgcgcggg ataacgtgca gtttattggc gacatggttc ccccactgaa
    47521 aatggtgggg gagctcacgg ggaagatggg ggcgctgggg ttagccggtg ctgccggcta
    47581 cggactgaaa caggtcgctt atggatttcg ggaggcatcc cgtcaggcct ataatcttga
    47641 tgtctcggca aaaaatgcgg gaatgcgcgt tgacgatttt acccgacttt ccggggcaat
    47701 gcgtattctt ggggcagaca gcgagagcgc taatgcatca atagaaggta ttttcaaagc
    47761 attcaatgag gctgccagtg gtaaaaacga gggggttatg gcagcgatgg cgcaaattgg
    47821 tgctcaaatc caaaaaaaca gcgatggttc agtaaatacc cttaaaacac tggagtctat
    47881 cgcaaaaatt tttccaacct tgcgacctga acagcagaag tccgccgctg atgcacttgg
    47941 gctgacgccc gaattgctgg cgctaatgcg tgacggtgag cgcatgaaaa agctgctggc
    48001 gaaatcggat gaatttggtc tgactgtgga tccggcacta aatcagcaat tgagtgaagt
    48061 gaacggcact atgaatgagc tcagcgcatc ctgggatggt ctgtggcaac gttcaaaaaa
    48121 caaggcactt aagaccattc tttcggatgg ttcagtcaaa gacggcctcg aaggtgttac
    48181 cgatctgttc actaatggtg attttactgg gctgtctcat gctctcggtt ttatcaacag
    48241 caatgatgct gagaaactac ggcgcattca gaacgataag gaactttata acagcttacc
    48301 ccgcagtgaa cgtgggcagg ttgacgcggg tttcatgact gatgctgtaa gaaagcggta
    48361 cgatgcgaat taccgcgcga ccgattctgc gattcaattg cagaatgact tatccgctat
    48421 cagccagcca caatccaacg ttgcacgcgg caatgttcct tacggggaaa caaggaataa
    48481 cgcaattggc ttcagaaata ataatcccgg taatttgagg gctgcagcaa acgcaacggg
    48541 taaaaatggc ggattttcta cctttgcgaa tgatgccgac ggaagagctg caatggcgag
    48601 acagctgatg ttgtatggtg acagggggaa taatactctg gatgggatta ttcataccta
    48661 tgctccgcaa tcagagaata atactcgtgc atatattgat tccgtatcaa aaagtactgg
    48721 gtttggagcg cagcagcgta ttaatctcca cgatcctgat gtgttaaaaa cactgatggc
    48781 agcaatgatt aaacatgaaa gcggagcaca gccgtatacc gaaaatgaac tgagcgattc
    48841 tgtccggacg gccatcattg acgatcggtg gaagggttta cgtagcccgg aagttctggc
    48901 tcaacagcga tatgatatta tctcaggttc gcaaactgga aatcgtgact ccagcactct
    48961 tagtaacccg agtgatgaaa ctgatattct ctcaggctcg cagaacagaa atcgggaatc
    49021 catcattctg agtgatacgg gtaagaaaag tgatgaaagt gtactcggcg acaatctggc
    49081 taagtctctt aaagaggcaa tgtcagaaca accacttaag ctcgaaatca caatggttaa
    49141 tgataagggt gagcgaaaaa cctataatgc ggaaaataat ggcagaataa caacggccat
    49201 gaattactga tcactgtcgt catttcgtta aggaagaagt tatgaatgaa aaagtttttg
    49261 gagcaaaagc catttatact ccaaaggttt ttgcgcttgt ttacctggta attggcattt
    49321 tcctcgtttt ttctgttgtc tcaatgaatt tcacggcgat tacgatatcg gtggtaagtg
    49381 cattgctttt acgtgtgctt tatgagttcc taatgaactc attcaaggcg actgagcatc
    49441 tttacaggat cgccgaatct cttgaccgta atggatccag cgataaatag ataagtcatt
    49501 tcagtgcata tgtaaaccgc cgacatggcg gtttttttat ttccggaggc gtgatgccgt
    49561 caattatcca ggacgcaata acttctcttt tggggggaga taccagcgat gactggcagg
    49621 ggcagttacg gcccagctca ttcagaggtg tgccatttgc aattgttgct gaggaaggga
    49681 gccacggccg acgtcaggcg gtacatgaat atccctaccg tgatacagcc tggatagagg
    49741 atatcgggcg ggcaacacgg cgatttgtta ttcgcggttt cttgatccag aacagccagg
    49801 tttacggcgg cggcgatgct atcacgcagc gccagtcact gattgaagcc tgtgaacaaa
    49861 aaggtagcgg tacacttgtc catccgacac tgggcgaatt aacggtttcc atccctgaga
    49921 atggtttgcg tatttccggt tcgatggaga acgggcgagt atttgaattt accctgatgg
    49981 caattgaatc agggcttaaa gtgtttgctg tcacgggcag taccgttgca ggcgccacgg
    50041 tgaaaaccaa ctatctgaaa ctggtcagca ctgctgtgct gagcacgatt gccagagtta
    50101 agagtgaaat ccgcggtgtc acacaggcta taaacaccat cagaggcacg gtcacgttct
    50161 ggactaacat ggttgacagc accatcagtc aggtcacgaa tctcagcaat gtcctgaact
    50221 ccacgttcgg gaatacccgg tacggacgtt acagtaaagg ctctgtgggc ggtagttcct
    50281 ctgctgttgc tggcaaatcg tcagttgctg atgtggatga tgagagagca ctggctgaca
    50341 aggtaacagc ccagtcggta atggaccgga aaaatgttac cgacaggtcg agccagctta
    50401 gcagctccaa cacacctgat gagtttgtcc agggcgtcgc cgacgtggta aacgcaattc
    50461 ttaacagcgc cggcagcgtt aatgaccgaa tcacagcgct ggaaaaactg gctaattcaa
    50521 tcagcacgga gtaccagcag tccgacagca gcaaagcgat ttcggcgacc atgaacacgc
    50581 tgattgttgt gctatgtact ggtgccatga ccagtgccgc tgcggactcc agaccagcca
    50641 gtacagacga ggcagaagag ttaactcaac gagtttctgt gcaacttgat acggcgctgg
    50701 ttctggctgg agaccgcgcg gacgatgata tgtataacgc gcttctcgcc gtcagatcgg
    50761 cattcctttc tacgatgagt gagcgtgctt ctggtctgag cgagcttctg caggttacta
    50821 ccgctcagcc gcttccggcg ctgacgctgg caaaccgatt ataccaggat gccacccgtg
    50881 cagatgaact ggtacaggaa gcgcgcgtac cgcatccggc gtttatgccg acaaccatga
    50941 aggtactgag gcaatgaatg cagacagcga tctggatgtt gtttctttga cggtcgacgg
    51001 caaaatcatc gaggggtggg attctgtccg ggtaacgcgg ggtattgagc gttttccctc
    51061 tgatttcgat cttgggctaa tggattactt ccctggcaac gaagatcgtc aactcgttga
    51121 agagggaatg tcttgtgaag ttcgtatcgg agatgatctg acactgacgg gatatgttga
    51181 tgactgggaa cccgcactat cgcgctcccg ccatgaggtc cgcgccacgg gcaggagcaa
    51241 atgtcaggac ctggtggatt gctcagccga gtggcctaac aacgtcatca atgccagtaa
    51301 tgcgcttgaa attgcttctc gcctggcatc ctactacggc atcaccgtaa ccacggatgt
    51361 tgatgaactt gtgaaggtac cccagttcac tctgaactgg ggtgagtctc cgcaagaagt
    51421 catcgatcgg gtggccagat ggtctgctct gctttactac gatcagcccg atggaaacct
    51481 gttactgacc cgggtgggaa cacgtcgtgc ggcaagtggg atagccgaag gggtaaatgt
    51541 cgagcaggca tactaccgca aatcgatggc tgacaggttt tcagattatg tcggtgtatc
    51601 aatgagcgtt tctccaattg cagggtattc gcctgatacg gcctatgacg ctgtgactct
    51661 ggcaacggcg agagatccgg aggccgcccg catgcggtac cgaaaacata tatcgattgt
    51721 ggaaagtacc ctgatggcta ctcaacaggc acaaagtgcg atcgactggg aaatgaaccg
    51781 gcggtacgga cgttcaaaac agctctcggt aaccatcgat tcctggcggg ataaagacgg
    51841 gaaactgtgg gaaccaaaca cattgatccc cgttgatctt cccaccttac ggttgccgaa
    51901 gactgaattg ctactggcag aagtcaccta tatgcgcgat gactacggca cccatgcacg
    51961 catgacgctg atgccgcctg aagcattctc cgttcagcca tatgccttct accagaacct
    52021 ggcgggattc aatacatgaa gcaactattt aaacatgcag cgaccaggat cgccggcatg
    52081 ctggggattg gccggatcac ggctatgaaa gatggtgggg tggtgcagtc tatccagtac
    52141 cagactccgc tggaggtggc cagcgctccg cggatggcag aatttggctt ttcatccggc
    52201 ctgccgtcag ggactgacgt ggttctggct tttattggcg gtgatcgttc cagcgcggtg
    52261 gtaattgcgt ccaaccatca ggggttccgt catacaggcc tgaaagcggg cgaaacggtc
    52321 atgtataacc agtggggcct taatattctc ctgacggaga aggggatctt cctggatgca
    52381 aagggccaga atgttgaggt caataacgcc actaacgtga ccatcaatgc cagccagggg
    52441 atccttgcaa ataccccgat cctgaggtgc acgggtgaca ttgttgataa ctgtgaaacc
    52501 aatacccgaa cactgaaaga gctgcgggat gcacataatg accatgatca tgtggttaaa
    52561 aatgcccaga gtggcaatga caatatccgc agccaaaaaa cagaggatca ggtgacatga
    52621 gtgacatcgc ttcattctgg aatgtggatg agatgtttgc tgactggcag aaagggctgg
    52681 gtgaactcac cacggggaac gatttacaga ctgcaatact ggacagcctg tttaccgaca
    52741 ggctggcgcg cgctgacgat aattatgagg atagcgatcg ccgcggctgg tggggggatt
    52801 ccggggagga atcccaactg ggatcccggc tgtggctgct acggcggaaa aaactgaccc
    52861 cggatgtagc aaaaaaagcg gaggaatact cgagtgaagc gctcaactgg ttaaaggttg
    52921 atggcgttgt cagcgaggtt attcctgttg caaggatcgt cctgcctgac cggctcaatc
    52981 tcattatccg ctatcaggca ccggggaagg actggcagga attcaggttt tactggatat
    53041 gggagcaacg ttaatatgcc gtttaaacga ccgacgctga gcgaactccg cgacggaaac
    53101 cggaaattta tgcaggcgga gcttgaggat gttggtgcgc tcctgcgctt cgcgaacctg
    53161 aaggtactgg ctgacatgga tgcggggatg gggcatctgc attacgccta ccttgactat
    53221 attgccctgc agacaaaccc gtttacctct accgatgagt atctcgccgg gtggatggcc
    53281 cttaagcagg tattcagaaa accagctgca gcggcgaagt cgcctgcggt acaggctagt
    53341 ggcagtgttg actgtattat ccctgttggc tcgatcatta accgcgggga cggataccag
    53401 taccggacgg atgcagatct taaaattcag gcagatggat ttggtatcgt cgcggtgacg
    53461 gccatactgc cggatattac cagtgatgta acgggtggag gcgcgcgcgg taacgctgat
    53521 gccgggacca taatgaccct ggacgcgaat attgctggcg tggatccaca ggtaacgtta
    53581 ctgtccgctg cgaccggcgg agccgatatt gaaacggaag aggattttcg cagtcgtggc
    53641 ttgctggcat ggcagaatcc gcctcagggt ggaagcgacg ccgattataa aaaatgggcg
    53701 cttgaggttt cgggcgtcac ccgcgcgtgg gtaaagcggc gtctgaacgg ggccgggacc
    53761 gttggcgtgt atatcatgtg tgatcggaat gacaatggtg ggtttccggt cggtaccgac
    53821 ggaatatccc aacttgagga ctggggggct gttaaagcca ccggagacca gctcgctgtc
    53881 gccgaccaca tctatccgca gcagacagac actgccattg ttttcgtatg ttccccgatc
    53941 aagaaagtca tcaatattga aatctctggg atcaaaaatg ccgacagcac cacagttcag
    54001 gggataaaag acgcgctgac ggcgctgttt tttgatgaag ctaaccctga tggttctggg
    54061 aaagtttacc tctctgatat taacgggagt atcggcggtg ttagcggcac gacgggctat
    54121 attcttaact ctccgacggc caatatcacc tttgctgttg gcgaaattcc ggtgcttggc
    54181 ggggtgaatt ttgtatgagc ctcttttcaa aaaatgatta tgccggtgcg cttggtgcgc
    54241 tgctaccgac gggcagggcg tggccccggt cgcaaagaac ggtacaggct gcggtattac
    54301 gggcactggg cagcgcgttt cagcgttctg acaacgatgc gcaaagcctg attactggtg
    54361 cttttccccc tacagcgacg gtaatgttgt cagaatggga aagctctctg gggttaccag
    54421 atgattgtgc gattggtgaa tccggtggcg tcagcgatcg ccagcgcgcc gtggtggcaa
    54481 agttaatcag caccggcggc ctgaaccgcg attattacat ccgggtggct gcagctcttg
    54541 gttataccat cactatcaca cagttccggc ccgctatgag tggcatgtca gtatgcggtg
    54601 atgcgcttaa cggtgacgag tggccattta cctggcggat aaatgcgcca caaacaacga
    54661 tcaagtattc gcttgctggc gcgtcctact gcggagatcc gctcgcatcg tggggcaata
    54721 aacaactgga gtgttcaatc aacaaaattg ccccatccca tctgaacatc attttcaatt
    54781 attcataact gatatttccc cctctgattt tatcgcttaa cactaagtga ggattaacta
    54841 tgctccgaat cgggcaagtc gaagccactg caacgcagga tggcaaatat actgatggaa
    54901 gtgttgctgg tggtattgcc gcaacgaggc tgcgggcagc agcgtttaat gccatgcagg
    54961 aagagttagc gcatatcgta gagtcagcag gattggcgct cgacattaac gatatgactc
    55021 aggttttaaa agccattcaa aaactcacac tgagccgtgc aaacccattt gccgatatca
    55081 aatcagatgg tgcagcggcg atttctacgg ctctcacaaa ccttggtttg ggagaagcgg
    55141 caaaacggaa tgtgggaaca ggggaaaatc agatacctga tatgtcagct tattcatcag
    55201 gatctggctg gcaaaaattg cctgatggtt caattgaaca gtggggaaga attaatttcc
    55261 cgaataacgc cgcggcggta tctacaaatg tgactttcac cattcctttt acgcaggagc
    55321 cagacgtagt gattgtatat gacggcggtt ttggtggtgg gaatatgtgg ggcgcaacta
    55381 actggacgaa aacaggtttc gttgctcatt gtaactatgg ctttgaaggt ggagccttct
    55441 acgcgaaagg gcgttaatta tgaaatattt gtatgttgat aatctggcgt acccttatgc
    55501 cctccagtct gtttacgctg caaaaggcca atggcccgaa ggtaaaggcg tggatataga
    55561 cgaagctatt ttcagggaat atttccatga tacgccgcct gacggtaagt acagaattac
    55621 cggagacgat ggaatgccag catgggcaga tgttccaccg ccaacacgtg aagaacaaat
    55681 tgcatcagcc gcaactaaaa agcagcaatt gattaatcag gccaacgatt atatgaacag
    55741 tagacaatgg g
//
LOCUS       DAOFQD010000016        54480 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.016, whole genome shotgun sequence.
ACCESSION   DAOFQD010000016 DAOFQD010000000
VERSION     DAOFQD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.016"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(69..773)
                     /locus_tag="QBF46_001015"
     CDS             complement(69..773)
                     /locus_tag="QBF46_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDQ6673235.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(910..1362)
                     /gene="moaE"
                     /locus_tag="QBF46_001016"
     CDS             complement(910..1362)
                     /gene="moaE"
                     /locus_tag="QBF46_001016"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDQ6673236.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1364..1609)
                     /gene="moaD"
                     /locus_tag="QBF46_001017"
     CDS             complement(1364..1609)
                     /gene="moaD"
                     /locus_tag="QBF46_001017"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDQ6673237.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPSVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1602..2087)
                     /gene="moaC"
                     /locus_tag="QBF46_001018"
     CDS             complement(1602..2087)
                     /gene="moaC"
                     /locus_tag="QBF46_001018"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDQ6673238.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2090..2602)
                     /gene="moaB"
                     /locus_tag="QBF46_001019"
     CDS             complement(2090..2602)
                     /gene="moaB"
                     /locus_tag="QBF46_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDQ6673239.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2624..3613)
                     /gene="moaA"
                     /locus_tag="QBF46_001020"
     CDS             complement(2624..3613)
                     /gene="moaA"
                     /locus_tag="QBF46_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDQ6673240.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDAQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3601..3745)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4010..4918
                     /gene="yvcK"
                     /locus_tag="QBF46_001021"
     CDS             4010..4918
                     /gene="yvcK"
                     /locus_tag="QBF46_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDQ6673241.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5110..7131)
                     /gene="uvrB"
                     /locus_tag="QBF46_001022"
     CDS             complement(5110..7131)
                     /gene="uvrB"
                     /locus_tag="QBF46_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDQ6673242.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7709..8386)
                     /gene="bioD"
                     /locus_tag="QBF46_001023"
     CDS             complement(7709..8386)
                     /gene="bioD"
                     /locus_tag="QBF46_001023"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDQ6673243.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLVLL"
     gene            complement(8379..9134)
                     /gene="bioC"
                     /locus_tag="QBF46_001024"
     CDS             complement(8379..9134)
                     /gene="bioC"
                     /locus_tag="QBF46_001024"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDQ6673244.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9121..10275)
                     /gene="bioF"
                     /locus_tag="QBF46_001025"
     CDS             complement(9121..10275)
                     /gene="bioF"
                     /locus_tag="QBF46_001025"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDQ6673245.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDAQREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10272..11312)
                     /gene="bioB"
                     /locus_tag="QBF46_001026"
     CDS             complement(10272..11312)
                     /gene="bioB"
                     /locus_tag="QBF46_001026"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDQ6673246.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11399..12688
                     /gene="bioA"
                     /locus_tag="QBF46_001027"
     CDS             11399..12688
                     /gene="bioA"
                     /locus_tag="QBF46_001027"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDQ6673247.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12747..13223
                     /locus_tag="QBF46_001028"
     CDS             12747..13223
                     /locus_tag="QBF46_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDQ6673248.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13375..14658
                     /locus_tag="QBF46_001029"
     CDS             13375..14658
                     /locus_tag="QBF46_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDQ6673249.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPEFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPT
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14892..17153)
                     /locus_tag="QBF46_001030"
     CDS             complement(14892..17153)
                     /locus_tag="QBF46_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDQ6673250.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAQKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     SKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNIPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVSRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(17336..18769)
                     /locus_tag="QBF46_001031"
     CDS             complement(17336..18769)
                     /locus_tag="QBF46_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDQ6673251.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(18845..19897)
                     /locus_tag="QBF46_001032"
     CDS             complement(18845..19897)
                     /locus_tag="QBF46_001032"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDQ6673252.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGEINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20081..21034
                     /locus_tag="QBF46_001033"
     CDS             20081..21034
                     /locus_tag="QBF46_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6673253.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSSNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(21075..22070)
                     /gene="pgl"
                     /locus_tag="QBF46_001034"
     CDS             complement(21075..22070)
                     /gene="pgl"
                     /locus_tag="QBF46_001034"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDQ6673254.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPMGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            22225..23043
                     /gene="ybhA"
                     /locus_tag="QBF46_001035"
     CDS             22225..23043
                     /gene="ybhA"
                     /locus_tag="QBF46_001035"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDQ6673255.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(23044..24102)
                     /gene="modC"
                     /locus_tag="QBF46_001036"
     CDS             complement(23044..24102)
                     /gene="modC"
                     /locus_tag="QBF46_001036"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDQ6673256.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKDICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(24105..24794)
                     /gene="modB"
                     /locus_tag="QBF46_001037"
     CDS             complement(24105..24794)
                     /gene="modB"
                     /locus_tag="QBF46_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDQ6673257.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(24794..25567)
                     /gene="modA"
                     /locus_tag="QBF46_001038"
     CDS             complement(24794..25567)
                     /gene="modA"
                     /locus_tag="QBF46_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6673258.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQFKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASVQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTIK"
     gene            complement(25734..25883)
                     /gene="acrZ"
                     /locus_tag="QBF46_001039"
     CDS             complement(25734..25883)
                     /gene="acrZ"
                     /locus_tag="QBF46_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDQ6673259.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            26012..26800
                     /gene="modE"
                     /locus_tag="QBF46_001040"
     CDS             26012..26800
                     /gene="modE"
                     /locus_tag="QBF46_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDQ6673260.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            26868..28340
                     /gene="modF"
                     /locus_tag="QBF46_001041"
     CDS             26868..28340
                     /gene="modF"
                     /locus_tag="QBF46_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDQ6673261.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            28602..29618
                     /gene="galE"
                     /locus_tag="QBF46_001042"
     CDS             28602..29618
                     /gene="galE"
                     /locus_tag="QBF46_001042"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDQ6673262.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANQPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            29628..30674
                     /gene="galT"
                     /locus_tag="QBF46_001043"
     CDS             29628..30674
                     /gene="galT"
                     /locus_tag="QBF46_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDQ6673263.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNETEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            30678..31826
                     /gene="galK"
                     /locus_tag="QBF46_001044"
     CDS             30678..31826
                     /gene="galK"
                     /locus_tag="QBF46_001044"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDQ6673264.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            31820..32860
                     /gene="galM"
                     /locus_tag="QBF46_001045"
     CDS             31820..32860
                     /gene="galM"
                     /locus_tag="QBF46_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDQ6673265.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            33065..33817
                     /gene="gpmA"
                     /locus_tag="QBF46_001046"
     CDS             33065..33817
                     /gene="gpmA"
                     /locus_tag="QBF46_001046"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDQ6673266.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(33975..35027)
                     /gene="aroG"
                     /locus_tag="QBF46_001047"
     CDS             complement(33975..35027)
                     /gene="aroG"
                     /locus_tag="QBF46_001047"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDQ6673267.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     HALLRQLANAVKARRG"
     gene            35343..35723
                     /locus_tag="QBF46_001048"
     CDS             35343..35723
                     /locus_tag="QBF46_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDQ6673268.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDEMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            35837..36778
                     /gene="zitB"
                     /locus_tag="QBF46_001049"
     CDS             35837..36778
                     /gene="zitB"
                     /locus_tag="QBF46_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDQ6673269.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(36775..37494)
                     /gene="pnuC"
                     /locus_tag="QBF46_001050"
     CDS             complement(36775..37494)
                     /gene="pnuC"
                     /locus_tag="QBF46_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDQ6673270.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(37532..38575)
                     /gene="nadA"
                     /locus_tag="QBF46_001051"
     CDS             complement(37532..38575)
                     /gene="nadA"
                     /locus_tag="QBF46_001051"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDQ6673271.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(38853..38928)
                     /locus_tag="QBF46_001052"
     tRNA            complement(38853..38928)
                     /locus_tag="QBF46_001052"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38893..38895),aa:Lys,seq:ttt)
     gene            complement(39061..39136)
                     /locus_tag="QBF46_001053"
     tRNA            complement(39061..39136)
                     /locus_tag="QBF46_001053"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39101..39103),aa:Lys,seq:ttt)
     gene            complement(39283..39358)
                     /locus_tag="QBF46_001054"
     tRNA            complement(39283..39358)
                     /locus_tag="QBF46_001054"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39323..39325),aa:Lys,seq:ttt)
     gene            complement(39362..39437)
                     /locus_tag="QBF46_001055"
     tRNA            complement(39362..39437)
                     /locus_tag="QBF46_001055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39402..39404),aa:Val,seq:tac)
     gene            complement(39489..39564)
                     /locus_tag="QBF46_001056"
     tRNA            complement(39489..39564)
                     /locus_tag="QBF46_001056"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39529..39531),aa:Lys,seq:ttt)
     gene            complement(39567..39642)
                     /locus_tag="QBF46_001057"
     tRNA            complement(39567..39642)
                     /locus_tag="QBF46_001057"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39607..39609),aa:Val,seq:tac)
     gene            complement(39779..39854)
                     /locus_tag="QBF46_001058"
     tRNA            complement(39779..39854)
                     /locus_tag="QBF46_001058"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39819..39821),aa:Lys,seq:ttt)
     gene            complement(40019..40810)
                     /gene="cpoB"
                     /locus_tag="QBF46_001059"
     CDS             complement(40019..40810)
                     /gene="cpoB"
                     /locus_tag="QBF46_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDQ6673272.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(40820..41341)
                     /gene="pal"
                     /locus_tag="QBF46_001060"
     CDS             complement(40820..41341)
                     /gene="pal"
                     /locus_tag="QBF46_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDQ6673273.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(41376..42668)
                     /gene="tolB"
                     /locus_tag="QBF46_001061"
     CDS             complement(41376..42668)
                     /gene="tolB"
                     /locus_tag="QBF46_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDQ6673274.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(42801..44066)
                     /gene="tolA"
                     /locus_tag="QBF46_001062"
     CDS             complement(42801..44066)
                     /gene="tolA"
                     /locus_tag="QBF46_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDQ6673275.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(44131..44559)
                     /gene="tolR"
                     /locus_tag="QBF46_001063"
     CDS             complement(44131..44559)
                     /gene="tolR"
                     /locus_tag="QBF46_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDQ6673276.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYSVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(44563..45255)
                     /gene="tolQ"
                     /locus_tag="QBF46_001064"
     CDS             complement(44563..45255)
                     /gene="tolQ"
                     /locus_tag="QBF46_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDQ6673277.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(45252..45656)
                     /gene="ybgC"
                     /locus_tag="QBF46_001065"
     CDS             complement(45252..45656)
                     /gene="ybgC"
                     /locus_tag="QBF46_001065"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDQ6673278.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(45806..46099)
                     /gene="ybgE"
                     /locus_tag="QBF46_001066"
     CDS             complement(45806..46099)
                     /gene="ybgE"
                     /locus_tag="QBF46_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDQ6673279.1"
                     /translation="MSKIIVTLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(46099..46212)
                     /gene="cydX"
                     /locus_tag="QBF46_001067"
     CDS             complement(46099..46212)
                     /gene="cydX"
                     /locus_tag="QBF46_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDQ6673280.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(46227..47366)
                     /gene="cydB"
                     /locus_tag="QBF46_001068"
     CDS             complement(46227..47366)
                     /gene="cydB"
                     /locus_tag="QBF46_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDQ6673281.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(47382..48950)
                     /gene="cydA"
                     /locus_tag="QBF46_001069"
     CDS             complement(47382..48950)
                     /gene="cydA"
                     /locus_tag="QBF46_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDQ6673282.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(49545..49772)
                     /locus_tag="QBF46_001070"
     CDS             complement(49545..49772)
                     /locus_tag="QBF46_001070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673283.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(49797..52430)
                     /gene="mngB"
                     /locus_tag="QBF46_001071"
     CDS             complement(49797..52430)
                     /gene="mngB"
                     /locus_tag="QBF46_001071"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDQ6673284.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVVDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLRLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEITHEAWQSRAVIR
                     YDMAVPRNLQERSVRQSTGRVGVELAVTLSDNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNDWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(52448..54364)
                     /gene="mngA"
                     /locus_tag="QBF46_001072"
     CDS             complement(52448..54364)
                     /gene="mngA"
                     /locus_tag="QBF46_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDQ6673285.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSTFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
BASE COUNT        13373 a        14534 c        13434 g        13139 t
ORIGIN      
        1 tgttaagtgt cgtttttagt aagaaaaaaa tcccgacaca tggccgggat attgatggtg
       61 aaaaagaatt aacggcggtt gccgaagatc cgcaacaaca tcaggaacaa gttgatgaag
      121 tccagataca aggttaacgc gccaagaatg gaatatttgc gcaggttcga cgtgtcgcga
      181 gtatctatct gctcacccat atttttcagt ttctgggtgt catacgccgt caatccgaca
      241 aagacaatca cgccgatgta ggtgactgcc cacatcaatg cttcgctttt cagccagaag
      301 ttgaccagcg atgccagcac aatgccgatt aacgccataa acagcatatt gccaaagccg
      361 cttagatcgc gcttcgttgt gtaaccgtac aggctcattg cgccgaacat cccggcagta
      421 acgacgaaag tactggcgat agaagcagcg gtatagacaa tgaaaatact ggaaagcgta
      481 agacccgtca gcgccgaata aagcataaag agcatagtcg ttacacctgc gctcagcttt
      541 tgaatcatcg ctgataacac aataaccaat gctaattgcg cgatgatcag accgattaaa
      601 aagacacggt tagtgaacaa cagctccatc acggccgcgg aattagccgc ataccaggca
      661 acaaatgcgg tcagcaacaa gccaacggtc atccagccat agacttgcgc catataagtt
      721 tgcaagccag cccggggttg tacaattgaa tcagaacgtg ggaatctgtc catgacgatc
      781 tcctgaagat ataaggaata tcttaaggat actgcaaaat gatgaggctg tgcatcgacg
      841 cagcgtaaac gcatgtactg agcggtgaaa ttgccggacg cagcggtgcc ttatccggct
      901 aacaaaaaac taccagcgtt ttgccgcctg ctgatcgctc tcccgagctt cgacccagcg
      961 gtcgccttcc ggcgtggctt cacgcttcca gaacggtgcg cgggttttga gataatccat
     1021 aataaactgc ccggcttcaa acgcactgct gcgatgcgca ctggtgacac cgacaaaaac
     1081 aatttcatcg cccggccata attccccgat gcggtgaatc accgtgacgc gccccagcgg
     1141 ccagcggtta cgcgcttcat cgacaatttc tgccagtgct ttttcagtca tccccggata
     1201 gtgttcgagg gttaatgctt tgacgctgtc gcccaggtta tggttgcgca ccttaccggt
     1261 aaaggtgact accgcaccgt cttcgtcacg ctccgccagc cacgggtact cttctcctac
     1321 gctgaacggc tgcggaccaa caacaatttt ggtttctgcc atcttaacct ccggttaccg
     1381 gcgggaagaa agctacttcg tcgccgtcag tcagcggatg gtcaaaactt accagcgtct
     1441 ggttgacggc agccagtaat ttgccatctt ccagcgccag cgcccagcga tcgctctgcg
     1501 cagccaggtg ctggcgtaac gcttcaacgg atgggaaatc cgcagccact tcggttgcat
     1561 ctgttcctac caactcgcgc acctgggcga aaaaaagaac tttaatcatc cgcttccacc
     1621 ttaaagtcac ccgacttgcc gccgctcttc gccagcaaac gtaccggacc aatcaccata
     1681 tctttttgca ccgctttgca catgtcataa atggtcagcg ccgccacgga ggccgcggtt
     1741 aacgcttcca tttcgacacc ggttttcccg gtcaggcggc ataaggtttc tatacgcacc
     1801 cgattgtgct ccggctcggc ctgtaaattg acttcaactt tgctgagcat cagcggatga
     1861 cagagcggga tcagatccca ggtgcgtttt gccgcctgaa taccggcaat acgcgcagtg
     1921 gcaaatacgt cgcctttgtg gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc
     1981 atggtgacaa aggcttccgc ccgcgcttca cgcacggttt ccgctttggc ggagacatcc
     2041 accatgtgcg cttcgccagc ggcgttgata tgggtcagtt gcgacatact tatttcttca
     2101 aatgtggatg gaaattacac ggacgcgtac gggcatccaa ctgcggcgcg atgatatttt
     2161 cccatgcggt acggcacgct ttggtcgaac ccggcatggc gaaaatcagc gttttgttag
     2221 cgacgcccgc taccgcacga gattgcaacg tggaagtgcc aatctcttca aacgacaaca
     2281 tacggaacac ttcaccaaaa ccttcaactt cacggtcgaa caacggcagc aatgcttcgg
     2341 gagcctgatc accttccgtc aggccagtac cacccgtaat caacaccact tgtacatcgt
     2401 cgctggcgat ccacgcagat acctgagcgc gaatagcgta gcggttttct ttcacaatgg
     2461 ctttatcgac aacgtgatgg cccgcttctt gcgccgaatc gcgcaaatag tgaccggagg
     2521 tatcgtcttc ttcaccgcgc cgattagaaa ccgtaagaat agcaatacgg gtcgggataa
     2581 attcagtgct tacctgactc atctgatctc tccttttgac gttttagccg ccaatgtacg
     2641 ataagttttg cgtaataccg gtgttgtttt gatgcaggaa atgggtctgt ttcttctccc
     2701 gcagcgccgc tgaaatacgc gcttccagcg cctgttgctg ggcatcgtct tccagcagat
     2761 cgcgcaggtt aacgccgcct tcaccaaaca ggcagagatg gagtttacca atggaggaaa
     2821 cgcgcaggcg gttgcaagtg gcgcagaagt ctttttcata cggcatgata aggccaatct
     2881 ctccggcgta atccggatgg caaaagactt gcgcgggacc gtcgctgcgt tgacgtaatt
     2941 ggtggatcca gccgcgacgc agtagctcgt cacgcagaac ctgaccagag atgtgatgct
     3001 tacggaagag ctcactgccc tcgcccgttt ccatcagttc gatgaaacgc agctggatgg
     3061 gacgatgctg gatccagttc agaaaggtgt cgagctggtg atgattaaca tcacgcatca
     3121 gcactgtatt gactttgacc ttctcaaaac cggcctcaaa tgcagcatcg atccctgcca
     3181 tgacctggtt gaatttatcc tgcccggtaa tagcgtgaaa ctggcgggcg tccagactgt
     3241 cgacactgac gttaatgcca gtaagtcccg catcgcgcca gttcgccaca tcgcgttcca
     3301 gacggtaacc attggtggtg accgcaatct ggcggatagc gtcgttttcc cgcacagcgg
     3361 cgatgatatc ggtaaagtcg cggcgtaaag acggctcacc gcctgtcagg cgcacttttt
     3421 cggtgcccag actggcgaag gcgcgcgtaa cccggcgaat ttcatcgacg gtaagaaagc
     3481 ctttattggt gacgccgctc ggtttgtagc catccggcag gcagtaggtg caacgaaagt
     3541 tacacacatc ggtaatcgac aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca
     3601 gttgtgaagc catgtacacc tttccagata cgggaggcga agtcatttct tccttcgccc
     3661 tggtggcaat ctttacgagc attgtcacgg ccaaagcacc gtatcagttg acccaggtgc
     3721 agaggctaga gtgtttagtg gttatgccga tactagcgtg taaatgagat ttttaccatc
     3781 cacactttcg ctatgtaatc atgtatatag cgtcatgatc gtgctatttc gctacagaat
     3841 aagggaaaat ggcggtttta ctttgatata catcatcagg gaggcatagc gtgcatcgtc
     3901 ctttagaggt agaagaaaat agtttgtatc ctcaatattg gcaggttaat tgctgtttcc
     3961 ccgcaatttg cgctactgta gcgcggataa tctgactcca ggaaactata tgcgcaatcg
     4021 tacgctggct gatcttgatc gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt
     4081 tctctcatca ctttcgtctt tgggttctcg tttaacgggt atcgtcacca ccaccgataa
     4141 tggtggctcg acggggcgta ttcgccgttc agaaggcggc attgcctggg gcgatatgcg
     4201 caactgcctc aatcagctga taacggaacc gagcgtcgcc tccgcgatgt ttgaataccg
     4261 ttttggtggc aatggcgaac tttccggtca taatctcgga aacttgatgt taaaggcgct
     4321 ggatcacctt agcgtgcggc ctctggaagc catcaattta attcgtaatc tgctgaaagt
     4381 ggatacgcat ttgattccaa tgtcagagca tcccgtcgat ctgatggcga ttgacgatca
     4441 ggggcatgaa gtttacggcg aggtcaatat cgaccagtta actacgccga ttcaagagtt
     4501 attattaacg ccgaatgtac ccgcaacgcg tgaggcggtt cacgctatca atgaagcgga
     4561 tctcatcatt attgggcctg gcagttttta taccagcctg atgccaattc tgctgctgaa
     4621 ggaaatcgcc caggcattac gccgcacgcc agcgccgatg gtctatatcg gcaatctggg
     4681 gcgtgagttg agtttacctg cggctaattt gaagctggaa agcaagctgg caattatgga
     4741 gcagtatgtt ggtaaaaagg tcattgatgc ggtcatcgtc gggccaaaag tggatgtctc
     4801 ggcggtgaaa gagcggattg tgattcagga ggtactggag gccagcgata ttccgtatcg
     4861 tcatgaccgc cagttgttac ataacgcgct ggaaaaggcg ttacaggctt taggttaacg
     4921 gaaatccgaa gaaaaattcc ggcttcctat tgaagacaaa gtgcgcgttg tttatgccgg
     4981 atgcggcgtg aacgccttat ccggcctaca aaccgcgcta attcaatgta ttgcagagat
     5041 catgtaggcc tgataagcgt agcacatcag gctgttttcc gtttgtcatc agtcttcttc
     5101 gctatcctgt tacgatgcgg caataaacaa ctcacgcaac tgatgcaact ggtcacgaat
     5161 ttgtgccgct tcttcgaact ccagattctg cgcgtgttgc atcatcagcc cttccagttc
     5221 atggattttc tgctgcaacg ctttcggcga catatccatc ggcacattat ccggctcaac
     5281 aatcgggcgc gattttcctc tgcccttcgc tttggttttg gcaatgttct gccccagcgc
     5341 caggatatcg accactttct tgttcaagcc ttgcggcgta atgccgtgtt cctcgttgta
     5401 cttctgctgt ttctcacggc gacgttcggt ttcgccaatc gctttcgcca ttgatggggt
     5461 gatcttatcg ccgtagagaa tcgctttacc gttaacgtta cgtgccgcac gaccaatggt
     5521 ctggatcaac gaacgttcgg aacgcaggaa gccttctttg tcagcgtcga ggatcgccac
     5581 cagcgacact tccggcatat ccagaccttc gcgcagtaag ttgatcccta ccagcacgtc
     5641 gaactcaccc agacgcaagt cgcggataat ctccatacgt tcgacggtgt cgatatctga
     5701 gtgaagataa cgcacgcgct cgccatgttc ttcgagatat tcggtaagat cttccgccat
     5761 ccgcttggtc agtgtggtga ccagtacgcg ttcgttaatt gccgctcgct gacgaatctc
     5821 cgaaagaaga tcatcaacct gtgtcgccac cggccgcact tcgataatcg ggtcaagcaa
     5881 tccggttgga cgcaccacct gatccaccac atcgccgccg gatttttcca gctcgtaatt
     5941 acccggcgtc gccgaaacat agatggtttg cggcgctaat gcttcgaact cttcaaactt
     6001 aagcggacgg ttatccagcg ctgatggcag gcggaagccg tactccacca gtgtctcttt
     6061 acgcgcccgg tcaccgcgat acatgccgcc aatttgtgga atggtgacgt gagattcatc
     6121 gacgaccagc agcccatcgg caggcaggta atcaaacagc gtcggcggtg gctcacccgg
     6181 tccacgaccg gagaggaagc gcgagtagtt ttcaatcccc gaacagtagc ccagctcgtt
     6241 catcatctcc agatcaaact gggtacgctg ggtcagccgc tgctcttcca gcagtttgtt
     6301 gttttccaac agcactttgc gtctggcggc cagttcttct ttgatctcct ccatcgcctg
     6361 tacgatgcgc tcgcgcggtg tgacgtagtg cgttttcggg tagatggtaa aacgtggaat
     6421 agtggaaaca atctgcccgg tcagcgggtc aaataacgac aatcgttcca cttcctcgtc
     6481 aaacagttcc acgcgaagtg caatgtcatc cgattctgcc gggaagatat caatcacctc
     6541 gccacgaacg cggaaagtac cacgctggaa tgcttgatca ttacgagcgt attgcagctc
     6601 cgccagtcgg cgcagaatcg cgcgctgatc gataatcata ccgaccgtga gatggagcat
     6661 catcttgaga tataaatcag gatcgcccag accataaatc gcggaaacag acgccaccac
     6721 aaccacatca cgccgctcca gcatcgcttt ggtggcggat aaacgcatct gctcaatatg
     6781 ttcgttaacc gaggcatctt tctcaatgaa agtgtcggaa ctcggtacat aggcttccgg
     6841 ctgatagtag tcgtagtagg agacgaaata ctccaccgcg ttttccggga agaactcttt
     6901 catttcgcca tacagctggg ccgccagcgt tttgttgggc gcaagtacca tggttgggcg
     6961 ctgaaggtca gcaatgacat tggcaatggt gaaggttttc cctgagccag tcacgccaag
     7021 taacgtctgg tgcgccaggc catcttccag cccctcttcg agacgtcgaa tcgcctctgg
     7081 ctgatcgcca gaaggtttaa aagcggaatt cagtttgaac ggtttactca tgagtcgcta
     7141 cctgaaggag ttgggcgggc aggtatgtaa ttttactcgt cgtacttaat tatgccaaca
     7201 attatactgg ataaaaaaac agttcatcac cataatattt ctgatacagc gtaaactccg
     7261 ctgtcaatca tgagcaaaat ttactctgtg gcgagataaa actccggcct taccgggtta
     7321 tccccaaagc aacggctttt ttaacatttg tcaagatgag tgatgacagt tttatggcaa
     7381 gagatgcctg ttcagtgact gccattgcat ttatctaacc agttaaaaaa taaaagatat
     7441 ttctttgagc cgtctttaac gccaattcgc gtgcaagccg cgtattctct cgcttgcctc
     7501 gtgttttcta actctaatac acatggttat ccacaggaat agtggataac tgtctccagc
     7561 ccctatcccc cgccgcctgg gcaattccca cattcgccgc atgaggcgga agtaaaattt
     7621 ttttcgtgat tattttagaa tttaattggt taaattgcag tcaatcgaag acgcgatctc
     7681 gctcgcaatt taaccaaata cagaatggct acaacaagac aaggtttatg tactttccgg
     7741 ttgccgcatt ttctggattt tctgcaagcc aggggatctc tcccagcagc ggcgcgggaa
     7801 tcatgcgggt gagcgtggtc atatattcag cgtgacgttt tcccggaggc gtaacatcgt
     7861 tcgccaccca acccgccaga gtcagtccgg cgtgttgtat tgcctgcgca gtcaacatcg
     7921 cgtgattaat acagccgagt ttcacaccaa ctaccagtat caccggcagt tgttcctgtg
     7981 ttacccaatc tgcaaaagtg aaagtgtcag aaagcggcgt aaaccagccg ccagcacctt
     8041 ccactaacac ccagtcagcc tgttgttcaa gcgcgcgtaa tccggcgctc attaccaatg
     8101 attctatcgg tctgccctct tgcgcgctga tgatgtgcgg cgaagtgggt tctgcgaagg
     8161 tgtaaggatt tactgttgcg taatccagct gcaggctgct gttgcgctgt aacgccagcg
     8221 cgtcgctatt gcgtaaacct tccggggtct tttcgctgcc agaggcgacc ggtttataac
     8281 ctgccgtccg gtagcctgct gcctttgcgg cttgtaaaag tgcacaactg gcgacagttt
     8341 tccccacttc ggtatccgtt ccggtgacaa aataacgttt actcacgagc aatcactccc
     8401 aaaaaaagat gatacgtcag aggatatcgc ccctgctgtt gcggccaggc cagttgcaat
     8461 cgctgcaact gcgaacgcgt taatattcgc gggtcgcgcc cttcatgaag atgcgtggca
     8521 ccgatgcctt tcagcgaacg catggcactg agcgcatcat caaaccacag cgtgatgggc
     8581 tgaatatgat gttgataatg cacgccgttc agcgactgtt cgatttcatc tggcggtaaa
     8641 aagcgattag catgcggacg ctcgtccacc gcctgccacg cctgatgcag ttcgggtaac
     8701 gatccctgca ccagcgtggt aaacgcgacc acgcctttgg ggcgcaccac ccgatacagc
     8761 tcgcggagtg ccgtggataa attaccgcac cactgcactg cgagattgct ccatgcaaga
     8821 tcgaacgtcg cagtcgctaa cggcagggat tcgatatctc ccgccagata atggtctgcg
     8881 gcatccttct ggcgtgcctg aacaagcatt ggcggcgaga gatctaaggc cgtcacctgc
     8941 gcgtgacgtt cccgccagtg gcggctcatc cagccaggtc cacaacccgc gtccagtacg
     9001 tgggtgtatt tacgctgtgg aagcattgcc agtaaggcgt cagcactctg gcgctgtaga
     9061 tctgcatgtt gctcatagtg tgcggctgcc cgaccaaatg ccgctgcaat ggcttgttta
     9121 ttaaccgttg ccatgcagca cctccagcag acggtcgata tcctgcattt catgcgcagc
     9181 ggttagcgtt aagcgcagtc gcgcagtacc agcgggtacg gttggcgggc gaatcgccgt
     9241 gacccagcag ccttgctggc gcagtttttc tgccagttgt aacgcacggc tgttatcacc
     9301 gacaatcaat ggctggatgg cgctgcatga atcagcaagc gtaaacggca aatcctgtac
     9361 tccggcacga aaacgcgtaa tgagtgccgc cagtttttcg cgctgtgcat caccctcatc
     9421 actgcgaatg accgccagcg acgcacgtaa tgcctgcgcc tgagcgggcg gcatactggt
     9481 gctgtagata aggtggcggg cgaattgcag cagataatcc gccaccgtac tggagcaaag
     9541 caccgctgcc ccgctgacgc caaatccttt gccaaaagtc actaccagca attctggttt
     9601 taccttttgc agccagcagc tgccgcgccc ctgctccccg ataacgcccg tgccgtgggc
     9661 atcatcgacc atcaaccagc cattgtgctg ttgcgttacc tgctggattt ccgccagtgg
     9721 cgcactatcg ccgtccatgc tgaacacgcc ttctgtcacc accatttgct gccccggaca
     9781 gggggaagca agcaatcgcg ccaaatgagt gacatcgtta tgagcaaaac ggcgaagctg
     9841 cgacgggctt aaactggcag cttccagcaa tgaggcatgg ctaagccggt cggcagcaat
     9901 acggtcctct ttcgccatca tcgcggcaat aactgcctga ttagcggcga aaccagagat
     9961 aaacagcagt gcccgcgaat agccaagcca ctcggccagc tcttcttcca gtgcctgatg
    10021 caccacgcta taaccgctga cgtgaccgga gccgccgcta ccgatgccaa attgctccgc
    10081 cccctgctgc caggcacgga taatttgcgg atgatggctt aaacctaaat aatcgttact
    10141 ggaaaagttc agatactggc gatcatccgc caccagccag cgtccggctc cttgcgccac
    10201 cggataacgg cgacgcaggg catcggcagc acgccgcgca tcgagcgccg cgttgatttt
    10261 ctcctgccag ctcataatgc tgccgcgttg taatattcgt cggtgtccgg ggtcatcagc
    10321 gcctgttcaa gacgttgctg ttgttcgtta tcccctgcca gcacggcagt ttgctgcgga
    10381 tttagcccca gtttgcggaa cagttgcagg tctttatctt cttccggatt cggcgtggtc
    10441 agcagtttgc aaccgtagaa aatcgagttt gcgcctgcca taaagcacat cgcctgagtc
    10501 tgttcgttca tctgctcgcg tccggcagaa aggcgcacgt aagaggttgg catcatgatc
    10561 cgcgcgaccg caatggtgcg aataaaatca aaggcatcga catcatcgtt atcggcaagc
    10621 ggcgtgcctt tcaccttcac cagcatgttg attggcacgc tttccggcgg cgtcggcagg
    10681 tttgccagtt gcagcaataa tccggcgcga tcttttaccg tttcgcctaa gcccacaatg
    10741 ccgccagaac agactttgat cccggcatcg cgcacttttt ccagcgtatc gaggcgttcc
    10801 tgataagtgc gtgtggtgat gatattgccg taaaactccg gcgaggtgtc caggttgtgg
    10861 ttgtagtaat ccagcccggc gttcgcgagg cgctgcgcct gagattcact caacgtgccc
    10921 agcgtcatac acgcctccag ccccatcgct tttaccccct gcaccatttg ttccaggtac
    10981 ggcatatcgc gttcgtgggg attcttccac gccgcgccca tacagaagcg cgtcgatcct
    11041 gccgctttcg ctttgcgcgc cgactccagc acctgttcaa cttccatcaa ccgctcggct
    11101 tccagcccgg ttttgtagcg cgagctttgc gggcagtatt tgcaatcttc cggacaagct
    11161 ccggtcttaa tcgacagcaa cgtgctgacc tgcacctgac gaggatcgaa atgctggcga
    11221 tgcacctgct gcgcttcaaa cagcagatcc agcaacggtt tttcaaataa ttctgtgact
    11281 tgcgacaatg tccagcgtgg gcggtgagcc atggggcttc tccaaaacgt gttttttgtt
    11341 gttaattcgg tgtagacttg taaacctaaa tcttttcaat ttggtttaca agtcgattat
    11401 gacaacggac gatcttgcct ttgaccaacg ccatatctgg cacccataca catccatgac
    11461 ctcccctctg ccggtttatc cggtggtgag cgccgaaggt tgcgagctga ttttgtctga
    11521 cggcagacgc ctggttgacg gtatgtcgtc ctggtgggcg gcgatccacg gctacaatca
    11581 cccgcagctt aatgcggcga tgaagtcgca aattgatgcc atgtcgcatg tgatgtttgg
    11641 cggtatcacc catgcgccag ccattgagct gtgccgcaaa ctggtggcga tgacgccgca
    11701 accgctggag tgcgtttttc tcgcggactc cggttccgta gcggtggaag tggcgatgaa
    11761 aatggcgttg cagtactggc aagccaaagg cgaagcgcgc cagcgttttc tgaccttccg
    11821 caatggttat catggcgata cctttggcgc gatgtcggtg tgcgatccgg acaactcaat
    11881 gcacagcctg tggaaaggct atctgccaga aaacttgttt gctcccgccc cacaaagccg
    11941 tatggatggc gaatgggatg agcgcgatat ggtgggcttt gcccgcctga tggcggcgca
    12001 tcgtcatgaa atcgcggcgg tgatcattga gccgattgtc cagggcgcag gcgggatgcg
    12061 catgtaccat ccggaatggt taaaacgaat ccgcaaaata tgcgatcgcg aaggtatctt
    12121 gctgattgcc gacgagatcg ccactggatt tggtcgtacc gggaaactgt ttgcctgtga
    12181 acatgcagaa atcgcgccgg acattttgtg cctcggtaaa gccttaaccg gcggcacaat
    12241 gaccctttcc gccacactca ccacgcgcga ggttgcagaa accatcagta acggtgaagc
    12301 cggttgcttt atgcatgggc caacttttat gggcaatccg ctggcctgcg cggcagcaaa
    12361 cgccagcctg gcgattctcg aatctggcga ctggcagcaa caggtggcgg atattgaagt
    12421 acagctgcgc gagcaacttg cccccgcccg tgatgccgaa atggttgccg atgtgcgcgt
    12481 actgggggcc attggcgtgg tcgaaaccac tcatccggtg aatatggcgg cgctgcaaaa
    12541 attctttgtc gaacagggtg tctggatccg gccttttggc aaactgattt acctgatgcc
    12601 gccctatatt attctcccgc aacagttgca gcgtctgacc gcagcggtta accgcgcggt
    12661 acaggatgaa acattttttt gccaataacg agaagtccgc gtgagggttt ctggctacac
    12721 tttctgcaaa caagaaagga gggttcatga aactcatcag taacgatctg cgcgatggcg
    12781 ataaattgcc gcatcgtcat gtctttaacg gcatgggtta cgatggcgat aatatttcac
    12841 cgcatctggc gtgggatgat gttcctgcgg gaacgaaaag ttttgttgtc acctgctacg
    12901 acccggatgc gccaaccggc tccggctggt ggcactgggt agttgttaac ttacccgctg
    12961 atacccgcgt attaccgcaa gggtttggct ctggtctggt agcaatgcca gacggcgttt
    13021 tgcagacgcg taccgacttt ggtaaaaccg ggtacgatgg cgcagcaccg ccgaaaggcg
    13081 aaactcatcg ctacattttt accgttcacg cgctggatat agaacgtatt gatgtcgatg
    13141 aaggtgccag cggcgcgatg gtcgggttta acgttcattt ccactctctg gcaagcgcct
    13201 cgattactgc gatgtttagt taatcactct gccagatggc gcaatgccat ctggtatcac
    13261 ttaaaggtat taaaaacaac tttttgtctt tttaccttcc cgtttcgctc aagttagtat
    13321 aaaaaagcag gcttcaacgg attcattttt ctatttcata gcccggagca acctgtgaac
    13381 acattttcag tttcccgtct ggcgctggca ttggcttttg gcgtgacgct gaccgcctgt
    13441 agctcaaccc cgcccgatca acgtccttct gatcaaaccg cgcctggtac ctcttctcgc
    13501 ccgattctgt cggcaaaaga agcgcagaat ttcgatgctc aacactattt tgcatccctg
    13561 acaccaggtg ctgcagcgtg gaatccttcc ccgattaccc tgcctgcgca acctgaattt
    13621 gttgtcggcc cggcgggcac tcaaggtgta acgcatacca cgattcaggc ggcggtagat
    13681 gcggcaatta tcaagcgtac caacaagcgc cagtatattg ccgtgatgcc tggtgagtat
    13741 cagggaacgg tatatgtccc tgccgctccg ggtggaatta ctctgtacgg tacaggtgaa
    13801 aaaccgattg atgtgaagat tgggctttcc cttgatggtg gcatgagccc taccgactgg
    13861 cgtcacgacg tcaacccgcg cggcaaatat atgccaggta aaccagcgtg gtatatgtac
    13921 gatagctgcc agagcaaacg cagcgacagt atcggtgttc tctgctctgc ggtcttctgg
    13981 tcacaaaaca atggcctgca actgcaaaat ctgaccatcg aaaacacgct gggcgatagc
    14041 gtagatgcag gtaaccatcc ggcggtggca ctgcgtactg atggtgacca ggtacagatt
    14101 aacaacgtta acattctcgg tcgtcagaac accttctttg tcaccaacag cggtgtgcag
    14161 aaccgtctgg aaacgaatcg tcagccgcgt acgctggtga ccaacagcta cattgaaggg
    14221 gatgtggata tcgtttctgg tcgcggcgca gtggtgttcg ataacaccga attccgcgtg
    14281 gtgaactcac gtactcagca agaagcgtat gtgtttgcac cggctacgct gtccaacatt
    14341 tactacggtt tcctcgccgt aaacagccgt ttcaatgctt tcggtgatgg tgtggcgcaa
    14401 ctgggccgct cgctggatgt tgatgccaat accaacggtc aggtggtgat ccgtgatagc
    14461 gccatcaacg aaggttttaa cacggctaaa ccgtgggctg atgcggtgat ctctaatcgt
    14521 ccgtttgcgg gtaataccgg cagcgtagat gataacgacg aaatacagcg caatctgaat
    14581 gacactaact acaaccgcat gtgggaatac aataaccgcg gcgtgggtag caaagtggtt
    14641 gcagaggcga agaagtaaga gctattaact atttgccgga tgcggcgtaa acgccttatc
    14701 cggcctacgg ttcgatgcga tttgtaggtc ggataagatg cgcaagcatc gcatccgaca
    14761 ataagtgccg gatgctgcgt aaatgcctta tctggcctac agattcgatg cgattcgtag
    14821 gtcggataag atgcgcaagc atcgcatccg acaataagta ccggatgcga cctacattca
    14881 tatggcgctt tttacatctg acggttttta ttgaagttaa tcaaactacc cgccttgata
    14941 atctcgcgct cttcggccgt caggctttcc atgtaaagcg taatttccgt taccggcgca
    15001 tcttcatgga tcacataacc tttaaacgtc gtacccggat tatccagcgc cgctttaatg
    15061 ccagggatgt aaatgtaatc ccccacttca aaggttggca cttccgccat ctgcagcggt
    15121 aacatccccc agttgatgac gttagaacgg tagcgtttcg tcgcgtactc ctcggcaata
    15181 ttcgccagac cgccaatcac acgctggcag ctcgccgcct gttcacgcgc agaaccatcg
    15241 cctggtttca ccgcatagac catgctgcca atttcagttt gcagcggatc aatatgctcc
    15301 tgaccagcaa tctgcttaat gcgcgcaaac acctctgtca gctcgctgac attccccgcc
    15361 agacgctgat tttccagctc agcagtcgct ttacttctgc taacataacc tggatcgcgg
    15421 cgtgacaggg taaactccgc cagaccaatc ggatttgaac gataagaaga ggtttcaccg
    15481 gaaggaatca gttcgtcggt ggtggtcact tcgtcgagga tcttcgagca cactttgagg
    15541 acgatattgt cagtcagcgc acccaattcc ggccagtctt taatgttcgg tccgtaaatc
    15601 agcggttgct gagtcgcccc tttcacaaag ccctgataaa cacggttttt atacggcgtt
    15661 acatcgaagg cgtactccgg tatgttgtcc cagcaatcga gttcgctggc agaggttaaa
    15721 tagccaccgt ttgccgcagt cgcagcgata gaacgagcgt ccatcaacgc caccgctgac
    15781 atctgcccat tagctggctt agagccttcg cggttcggga agttacgcgt ggtgtggcga
    15841 atactcaaac cgttgttgat tggcgtatcg cccgcgccaa agcatgggcc gcagaacgcg
    15901 gttctgatga ttgcgcctgc gccaatcaaa tctgctacca cacctttttt ggctaaatcc
    15961 ataaacaccg gctgtgatga cgggtaaacc gccagcgaga aggtgtcatt gccacaggat
    16021 tgaccgcgca gtgcattcgc cgccgcgatg acgttttcgt agttaccgcc agaacagccc
    16081 gcgataatcc cctgctgcac tttcaggcga ccattttcca ctttatccag cagcgagagt
    16141 ttggctttac tgtgcgccac gcgttcggac tcaatttcaa tctcacgcag aatgtcggtc
    16201 aggttctggt tcagtgtgtc gatttcatac acgttgctcg ggtggaacgg cagcgcaatc
    16261 attggtttga tggcgcttaa atcaacgctg atgcagccat cgtagtacgc catcggttga
    16321 gggttaagct ggcagtaatc ctggccgcga ccgtgcagcg ccagccagtt atgcacttct
    16381 tcatcggttt gccagacaga acttaaacag gtcgtttcag tggtcataac gtcaacgctg
    16441 ttacggaaat cggtagagag cgcagcaaca ccgggtccta cgaattccat cactttgttt
    16501 ttgacgtagc cgtttttgaa cacggcaccg atgatagcca gcgccacatc ctgcggcccc
    16561 acatacggcg ctggttttcc ggtcagatgc accgcaacca cgcccggata gtcgatatcc
    16621 caggtgtcat tcagcagctg ttttaccaac tcaccgccgc cctcaccgac tgccattgtc
    16681 cctaatgcac cgtaacgggt gtggctgtct gacccgagga tcattttgcc gccgcctgcc
    16741 atcatctcac gcatatattg atggatgacc gcaatgtgcg gaggcacaaa aataccgcca
    16801 taacgctgag ccgccgataa accaaaaaca tggtcatcac cgttaatggt gccaccgacg
    16861 gcgcaaagtg agttatggca gttggtcagc acatacggca gcgggaaacg ttccataccg
    16921 gacgctttag cggtctgtac aataccaaca aaggtaatat cgtgagaggc taatgaatca
    16981 aacttaattt taagtttatc catatttccg gacgtattat gagaagagag aatagaccag
    17041 gcaatagtgc ctttttgggc ttcttctttt ttaatttcgc cggtgaaatg ttcttcggca
    17101 attatttcgt tattactggc gagaaacacg cctttttcag ataacttgat catcaaatgc
    17161 tccagatatc tgcgcaccgt gaagtgcgca gaaagataat aaatggatat actctaaata
    17221 attcgagttg caggaaggcg acaagcgagt gaatccccag gagcttacat aagtaagtga
    17281 ctggggtgag cgaacgcaga cgcagcacat gcaacttgaa gtatgacgag aatatttaca
    17341 gcatgttcca gccgatcagc atattccacc accacacgcc gagggtgata tgcaccagga
    17401 aggttaatat cgtcagtacc gcaccgacca accaccagga tttaatatcg ttataaccca
    17461 cgccaaagat gaccggacct gccgcgccgc catagtgagt aaccatgccg ccataggagt
    17521 tggagaacaa cagtgccagc gcggttaaca ttaacggtgc gccggagacg ttcgccagca
    17581 tggcaaatac cggtaacata gcaacgatat aggcactacc ggaagcgaag aaataacgca
    17641 cgataatgct gaggaaaata ataacgaaga aagcaacgtt accgtgacca tcaaatgcca
    17701 ggttattttt aaagacttca gctaaccatt cgaagaattt aactttcgat aataaggagc
    17761 ttaatccgat aataccgccg taccagatta aggtattcca gccgccttta tttttaacca
    17821 cgtcttccca ggtaacgata cccagcagca gcatggtcgc cataacaacg attgccacag
    17881 tggattcatc aacccccaga gacttactga aaatccagcc cagtagcgcc agcacaaaga
    17941 caccgagcag cattttttcg cggattttca tcggtcccag ttcggcaagg cccgctttag
    18001 cgatggtttt gttatccacc tttttaattt ctggtggata catggtgtaa attaccagcg
    18061 gggtgaccag cagcataatg atgcccggca atccggcggc cagcgcccat ccaccccagc
    18121 taatttgcag gtgcagaatg tcgttgatca ttttcagcgc cagaatgttc cccgccattg
    18181 cggtaaagaa catatagctg gtggttttgg tgaccatgta aatggacatc atcaggtaat
    18241 gtccgacacg acgcggactt ttttccggtt cggaccccaa agccaccgcc acgctgttga
    18301 tgatcggtaa cacaataccg cccgcacgcg cggtgttaga cggtgttgcc ggagccagta
    18361 ccagatcgag gaataccgta acgtaaccca gacccagcgt ggtgttaccg attttaccaa
    18421 tcagcagata ggcaatacgt ttacctaaac cggtggtcac aaatgcggcg cttaaggtaa
    18481 acgccgagaa caccagccag gtagtacctg aagagtaacc gcttaatacg gcggtggtct
    18541 taaacgcacc gtcggataag ttaccgacca ccaccattga ggcagcaacg gcaattaaca
    18601 gtacgacagg ttccgggaaa ggcttgataa ccagacccac aatggccgcc aggtaaatac
    18661 caaaaagcac ccacgccagt tcgcttaatc ctgccggagc tggcataaag ccaataatac
    18721 atgggatcgc taatatcaga attagcttcc ataacgattt cttattcatt taaaatcctg
    18781 ttctttctgt atgactcata cagaagtcct ttattgattg tccttatcct gacaacgaat
    18841 tttttcaggg aagataaact tcaccggaaa atatttttct ggtcgtccga ataacagatg
    18901 cgcgtaacgt cgtggcatcc tgaccttcat tacttaaatg aacgtcgagc gcaccactgg
    18961 ggtgttcaat attgatattg ccgtatccta cagaagggac gatttgtcgg gtgacggtgc
    19021 cttccaatgc acaactactg gaaatagcaa tagcaccggt tatcgccagc gcgcgatggc
    19081 aagaatgcgg cataaaataa cgcacattaa tttccccgcc tttctgcgct ggagaaataa
    19141 gcacaggttt agggataacc atattactga catcacctaa gcccattgct ttacccgctt
    19201 gtagacggat agattcaatg cgggctaata atgctttgtc ggcatccagt tccgccggta
    19261 attcataacc tgtttttccc agatattcag ccggaataat gacgactggc atcgccatat
    19321 cgatacaggt caccgggaca tcgtcaaaat aatcaatctg attatcagtc gggaaaactt
    19381 ttccggtttt ggttccagcg gcattcagga aagtgagcgc aaccggtgcg gcagtacccg
    19441 gtacgccgtc aattctggca ctaccctcgt actcgacaac accatttggc gtttgcacat
    19501 cagcttcgat gaacgtaccc gtattgacgt tgcggatacg cacacgggtg accggtgatg
    19561 tcgctgcaat caaaccattt tcaatggcga atgccccaac gccagaaagc atattgccgc
    19621 agttaggcgt ggtatcgaca cgttgctcat ggacgataac ttgagcaaac agataatcaa
    19681 catcagcacg cggatcgctg gaacggctaa taatggcgac tttactggtc agtggattac
    19741 cgccgccaat accgtcaatt tccagatcgt taccggaacc cataattgcc atcaatattt
    19801 tatcgcgctg cgtttgatct tcgggtaaat gttccgctaa caggaacgcg cccctcgagg
    19861 ttccacctcg catcatcacg cagggtattt ttttcataat aatgccctca atttaaaaac
    19921 ataacttatt gcgatatggt tacattaagg acaaagcatc tctaatgact ttagctcaac
    19981 gattaataac attttttcat taatttaagt tggctaaatg cagttaaaat ttttaacgac
    20041 cagccaccca aaatcgtcgc ttccattttc cggagtaata atgaagcatg aattatcaag
    20101 tatgaaggca tttgtcatac tggcagagtc cagttcattt aacaatgctg ctaaattact
    20161 caatattacg caacctgcat taacgcgaag aataaaaaag atggaggaag atttacatat
    20221 tcaactattt gagcgtacaa ctcgcaaggt tacgttaaca aaagcaggaa aaaggttgct
    20281 cccggaggcg cgggaattaa taaagaaatt cgatgaaacg ctttttaata ttcgtgatat
    20341 gaatgcttat catcgtggta tggtgacgtt agcgtgtatt ccgaccgcag tattttattt
    20401 tttaccgctg gcaatcggta aatttaatga gctgtatccc aatattaaag tgcggattct
    20461 ggaacaaggc acgaataatt gcatggaatc ggtgctgtgt aacgaatcag actttggcat
    20521 aaacatgaac aacgtcacaa attcatccat tgattttacc ccactggtca acgagccgtt
    20581 cgtgctggcc tgtcggcgtg accatccatt ggccaaaaag cagcttgtag aatggcagga
    20641 gttagtgggt tacaaaatga ttggcgtacg ctcctccagc agcaaccgac tgttgataga
    20701 acaacagctg gccgacaagc cctggaagct ggactggttt tacgaagtgc gtcatctttc
    20761 gacgtcatta ggactggtgg aggcgggact ggggatttcg gcgcttcctg gcctggcaat
    20821 gcctcatgcg ccctattctt cgattattgg tatcccattg gtggaacccg ttatacgtcg
    20881 aacattggga ataattcgcc gtaaagatgc cgtactttct ccagcagcag aacgcttttt
    20941 tgccttgctg attaatttat ggactgacga taaagacaat ttgtggacca atatcgtcga
    21001 acgccagaga cacgcgttac aggagatagg ttaatacacc ggcgcaggag agactgcgcc
    21061 gggtaaatca gcggttagtg tgcgttaacc accacccaca ttggtccctg cccgaccgca
    21121 tagcggcctt tttcatgcag tagcccctgc tcgccaacaa tttcgtatac cgagatgtgg
    21181 tgagattttt gcccagcggc aatcagatac ttgccgctgt gatcaacatt gaagccgcgc
    21241 ggctgggttt ccgttggctg gaagccttct ttactcaaca cgctgccatc ttccgaaacg
    21301 ctgaagacag taatcaggct ggcagtacgg tcacaggcat ataagtgacg cccatccggg
    21361 gtgatatgaa tatcagccgc ccaacgggta tcggagaagt tttccggcat catatccagc
    21421 gtctggacac attcgatatt gccgtgcgga tctttcagtt cccagacatc cactgagctg
    21481 tttaactcat tgacgcaata cgcatattgt tcgtttggat ggaataccat atgacgcggg
    21541 ccggcccctt caacggtagt cacttccgca gggtcctgcg ccaccagatg accatcatcg
    21601 ctgaccgtaa acaggcaaat gcgatcctgc tttaatgccg gaacccacag cgtacggttg
    21661 tccggtgaga tattggcgga atggcaaccg tccaacccct cgaccacatc gacgacgccc
    21721 attggcaggc catcttccag acgcgttacg ctcacgttac ccgcattgta agaacctaca
    21781 aagacaaact gcccctggtg atcggtggaa atatgcgtcg gactacccgg cagcgcagac
    21841 tctgcggcaa aggtcagtgc gccatcgtcc ggggcgatac gatacgccag gacgcgaaac
    21901 tcagggcgaa caccaacata gagataacgt ttgtccgggc tgaccaccat cggctgcacc
    21961 tgccccggca catcgacaac ctgtgtcagc gtcagtgcgc cttcatgatt caggttccag
    22021 acgtgaattt gctggctctc agggctggcg atataaactg tttgcttcat gaatgctcct
    22081 ttgcatttag caactgactg taacagctaa aattagtcgc ttttggcggt gaatgctgaa
    22141 ttaaaacgca gaattttgtc cgatagtggt atcggtgtac cattcgcgca gacgaaaatt
    22201 cttaacctta atctggaata cgccatgacc acacgcgtga ttgctcttga cttagacggc
    22261 accttattga ccccgaaaaa gaccctgctt ccttcatcga tagaagccct ggcccgcgct
    22321 cgcgaagcag gctatcaatt aatcatcgtt acaggtcgcc atcacgtcgc tattcatcct
    22381 ttttatcagg cgctggcgct ggatacacct gctatttgct gtaatggcac ctatttgtat
    22441 gattatcatg caaaaaccgt gctggaagcg gacccaatgc ccgttaataa agccctgcaa
    22501 ctcattgaga tgctgaatga gcaccacatt cacggtctga tgtatgtgga tgatgcaatg
    22561 gtctatgagc acccgaccgg gcatgtcatt cgcacgtcta actgggcgca aaccctgccg
    22621 ccggaacagc gtccgacttt cacacaagtc gcttctctgg ctgaaacggc gcaacaagtt
    22681 aacgccgtat ggaagttcgc cctgacgcac gatgacctgc cgcaattgca gcattttggt
    22741 aagcatgtcg aacatgaact gggactggag tgtgaatggt cctggcacga tcaggttgat
    22801 attgcacgcg gcggcaacag caaaggtaaa cgtttgacga aatgggttga ggcgcaaggt
    22861 tggtcgatgg aaaacgtcgt ggcattcggc gataacttta atgatatcag tatgctggaa
    22921 gccgctggta caggcgtggc gatgggcaac gccgatgacg cggtaaaagc gcgcgccaac
    22981 attgtgattg gtgataacac caccgacagc attgcccagt tcatttatag ccacctgatt
    23041 taatcaggcg gttatcgata cacttttaat ttgcgcgtac agccacaggc caggtttgat
    23101 cgccagttca tccctggccc acgggctgat acgcgcccac agcgttttac cgccgacttc
    23161 cagctccact tccacctggc cgttgtcgtc ataactatta acaacttttg cccgcaatac
    23221 gttacgaatg ctggtttgct gcggcggttg taaaaccaga gaaacatcgg aagcctgaat
    23281 gcggatacgt agcgcagctt gcagcggttc gtccagctta ttgacccaca aatgctgatc
    23341 gcccagcgcc agcgcagtca tcgcgtaatg cggatgatgc tcaagtaccg tcactttcag
    23401 aatgctactt tgttgctctt tcggcagcca cggattcatc acgctactcc cccagacttc
    23461 ttccagcgcg ccaaaggctt tcacctgacc gttttccagt accatcactc tgtctgccag
    23521 atggaggatc tcatccagcg aatggctgac atacaacatc gggatgttga tttcccgcgt
    23581 cagacgttgc agataaggca acagttcgcg tttacgcgga atatccagtg acgccagcgg
    23641 ttcatccagc aacagcaatt ctggtgccgt cagcaaagcc cgaccaatcg ccacgcgctg
    23701 tttttcgcct ccggacaggc tgcctggtaa acggtcaagc aacggttcaa tgcctaaaag
    23761 cgccaccagc ttatcgaact gatcgaccat acttttcgac atgccgtagc gcagattgcc
    23821 acgcactttg taatgcggga acagccgcgc atcctgaaaa acatagccaa cgcgacgctt
    23881 ttccggcgtc aggcagatat ctttttcggc atcatttagt acccgcccat tgaggacaat
    23941 ccgccctttt tgcgggcgcg tcagtccact gatggcgtta atcagcgaag tttttccggc
    24001 accggagacg ccaaagatag cagtgatgcc attggcgggc agcgtttcat taatggtcag
    24061 gcaatggttg cccaacgtct gggaaaaatt cagctccagc atgattagcg ccccgcccgt
    24121 tcacggctga ttctggccag ccattctgaa atcaacaggg agatcatcgc cagtgcaata
    24181 gaaataatgc acagtctcgc cgcgccactt tccccgccgg gggtctggat cagggtatac
    24241 atggcagaag gaatggttcg cgtttcacca ggaatgttcg acacaaaggt gatggttgca
    24301 ccaaactcac cgagagaacg agcaaaagcc agtaccgtac caacaataat gcccggtaag
    24361 gtcagcggta acgtgatagt aaagaaaacg cgccagcgcc cggcacctag tgttcttgcg
    24421 gcctgttcca gtttgacatc aaccccttcc agcgccagac gaattgcccg caccatcagc
    24481 ggaaacgaca tgacggcggc agcgagaacc gcgccgcgcc agctaaaggc gaaggtaata
    24541 ccaaaccagt catacagacg ttcaccgata aatccgcgcc gtcccatcga aactaataat
    24601 aagtaaccga cgaccacggg cggtaacacc agcggtagat gcagtacgct gtcgagcaga
    24661 gctttgcccg gaaacgtgca acgcaccagt aaccaggcaa aaaagatccc aaacggcagg
    24721 ctaaacagca cagccaggga agaaactttc aggcttaata aaactgcctg ccattctgga
    24781 tcggtcagta tcattacttg attgtaaatc cgtaacgttt aaagatttcc gctgcctgcg
    24841 gtcccttcag ataatcataa aatgctttca ctgtcgcatt gttatgccct tccacaacag
    24901 caaccggata ttccactttt ttatgtgaat cttccgggaa ggtggcaacc acttttaccc
    24961 ctttgctggc aactgcgtca gaaccgtaga caatgcccag aggcgcttcg ttacgttcga
    25021 ccagcgccag cgccccacga acatcttccg ctggggccag tttcggagag agcgtatccc
    25081 atgcgcccag tttttgcagt gcttcttttg cataaatgcc agcgggaaca tgttccggat
    25141 cgccaaccgc caggcgaccg ccattcagca gtgaagtcca gttggttttg ctgtcgatgg
    25201 tgaaatcttt ctgcacgctg gctttcggtg ctacaacgac caggctattg ccgagcagtg
    25261 tctgacgcgt agctgtatcg atcgcttttt tatcaaccgc ataatccatc catttctgat
    25321 cggcagaaat aaacagatcc gcaggcgcac ccgcttcaat ctgacgggcg agagtagatg
    25381 acgaagcgaa agaagaaacc acatccacgc ctttctcttt tttaaactgc gtagcaatgt
    25441 cctgcattgc gttagtcagt gatgcggcgg cgaacaccgt gattttccct tcatctgcca
    25501 gtgcattgcc agcaacagcg aaagagagtg ccgccccggc aaacaagttc aaccatttac
    25561 gagccattgg taacccctta atgtaacgtt atgtaggcga caatataacg actaactcgg
    25621 aattttccca gcagttattg ctaacctttg tgaggtagat aagaaaaaat atcggcagga
    25681 aaagcaggaa gttgagaaaa agaaaatgcc cgactaagcg ggcattcagg gaatcaatga
    25741 ttttgtccgg gctggtcttt tttaccaaca ccagaaaaga tgttgaatac ttcaccaaga
    25801 ccgtaaatca gacccaggat gatggccatc acgacaggta ccatgattac ggcgaatacc
    25861 agacttttta ataactctaa catggtcaac tccagatata gtcatgagac tattctaacc
    25921 gctaagcaca gaaaagcact ccccttttgt gcggtcagct ttgcgtggcg ttcgttttcc
    25981 gtcacaataa gacttttgcc aggacattgt tatgcaggcc gaaatccttc tcacccttaa
    26041 gctccaacaa aaattattcg ccgacccgcg ccgcatttcg ctactaaaac acattgcgct
    26101 ttccggttcc attagccagg gagcgaaaga tgccggtatt agctataaaa gcgcctggga
    26161 tgccattaac gagatgaatc agttaagtga gcatattctg gtcgagcgcg caacaggcgg
    26221 taaaggtggc ggaggcgcag tactgacccg ctatggtcag cgactgattc agctctatga
    26281 cttactggcg caaatccagc aaaaagcctt tgatgtgtta agtgacgatg acgccctgcc
    26341 gctgaacagc ctgctggccg cgatctcacg tttttcactg caaaccagcg cccgtaacca
    26401 gtggttcggt accatcaccg cccgcgatca tgatgacgtt caacagcatg ttgatgtctt
    26461 actggctgac ggaaaaacac gcctgaaagt cgcaattacc gcacaaagcg gcgcacgtct
    26521 ggggctggat gaaggcaaag aagtgttgat attgctaaaa gcgccgtggg taggcattac
    26581 tcaggacgaa gcggtcgcac aaaacgccga caaccaatta ccgggtatta ttagtcatat
    26641 tgagcgcggc gcagagcagt gcgaagtatt aatggcgcta cccgacgggc aaacactgtg
    26701 cgccacagtg ccggtaaatg aagcgacttc tcttcagcaa gggcagaatg tcacggccta
    26761 ctttaatgcc gacagcgtga ttatcgccac gctgtgctaa gcgtgttgac aatttgttat
    26821 gaaacacgta tccctgtcag taatcgctgc acaaaatggg atataaaatg tcatcgttgc
    26881 aaattttgca aggcacgttt cgtcttagcg acacaaaaac gctgcaattg cctcagctaa
    26941 cgttaaacgc gggtgatagt tgggcgtttg tcggttcgaa tggaagtgga aagtcggcgc
    27001 tggcccgcgc gctggcgggg gaacttccgc ttttgaaagg tgaacggcaa agccagtttt
    27061 cccacatcac tcgtctctcc ttcgagcaat tgcaaaagct cgtcagcgac gaatggcaac
    27121 ggaataacac cgatatgctc ggccctggcg aagatgacac cggacgcact acggctgaga
    27181 ttattcagga tgaagtaaag gatgcaccgc gttgcatgca actggcgcag cagttcggta
    27241 ttaccgccct cctcgaccga cgctttaaat acctttccac tggcgagacg cgaaaaaccc
    27301 tgctgtgtca ggcgctgatg tcggagcctg acttgttgat tcttgatgag ccgttcgatg
    27361 gcctggatgt tgcctcacgt cagcagctgg ctgagcgact cgcctcgtta catcagtccg
    27421 gtattacttt ggtactggtg ctcaatcgct tcgatgagat cccggaattt gtccagtttg
    27481 ctggcgtgct ggcggattgt acgttagcgg aaactggcgc taaagaggaa ctgctccagc
    27541 aagcactcgt cgcgcaactt gcgcatagtg aacagcttga aggtgtgcaa ctgccggagc
    27601 cggatgaacc ttcagcacgt cacgccttac ccgccaacga atcgcgcatt gtgctgaaca
    27661 atggcgtggt ttcttataat gatcgcccca ttcttaataa ccttagctgg caggtgaatc
    27721 caggcgaaca ctggcaaatt gtcgggccaa atggcgcggg aaaatcgacg ttattaagcc
    27781 tgattactgg cgatcatccg caaggttaca gcaacgattt gacgcttttc ggccgacgtc
    27841 gcggcagcgg cgaaaccatc tgggacatca aaaagcatat cggttacgtc agcagtagtt
    27901 tgcatctgga ttaccgggtc agcactaccg tgcgtaatgt gattctttct ggctattttg
    27961 attcgattgg catttatcag gccgtttcgg atcgccagca aaaactggtg cagcagtggc
    28021 tggatattct cggcattgat aaacacacgg ctgacgctcc gttccatagt ctttcctggg
    28081 gacagcagcg tctggcgctg attgtccgcg cactggtgaa acatccgacg ttgcttattc
    28141 tcgatgaacc actacagggg cttgatccgc tcaatcgcca gcttatccgc cgttttgttg
    28201 atgtgctgat tagcgaaggt gaaacgcaat tgttgtttgt ttcgcaccac gctgaagatg
    28261 cgcctgcctg tattacccat cgtcttgagt tcgtgccgga cggtggactc tatcgctatg
    28321 ttctgacaaa aatatattga gtcggtagtg ctgaccttgc cggaggcggc ctaagcgccc
    28381 tctccggcca acggttcgac gcatgcaggc attaaaccgc gtcttttttt cagataaaaa
    28441 gcgcaatcat tcataaaccc tctgttttat aatcacttaa tcgcgcataa aaaacggcta
    28501 aattcttgtg taaacgattc cactaattta ttccatgtca cacttttcgc atctttgtta
    28561 tgctatggtt atttcatacc ataagcctaa tggagcgaat tatgagagtt ctggttaccg
    28621 gtggtagcgg ttacattgga agtcatacct gtgtgcaatt actgcaaaac ggtcatgatg
    28681 tcatcattct tgataacctc tgtaacagta agcgcagcgt actgcctgtt atcgagcgtt
    28741 taggcggcaa acatccaacg tttgttgaag gcgatattcg taacgaagcg ttgatgaccg
    28801 agatcctgca cgatcacgct atcgacaccg tgatccactt cgccgggctg aaagccgtgg
    28861 gcgaatcggt acaaaaaccg ctggaatatt acgacaacaa tgtcaacggc actctgcgcc
    28921 tgattagcgc catgcgcgcc gctaacgtca aaaactttat ttttagctcc tccgccaccg
    28981 tttatggcga tcagcccaaa attccatacg ttgaaagctt cccgaccggc acaccgcaaa
    29041 gcccttacgg caaaagcaag ctgatggtgg aacagatcct caccgatctg caaaaagccc
    29101 agccggactg gagcattgcc ctgctgcgct acttcaaccc ggttggcgcg catccgtcgg
    29161 gcgatatggg cgaagatccg caaggcattc cgaataacct gatgccatac atcgcccagg
    29221 ttgctgtagg ccgtcgcgac tcgctggcga tttttggtaa cgattatccg accgaagatg
    29281 gtactggcgt acgcgattac atccacgtaa tggatctggc ggacggtcac gtcgtggcga
    29341 tggaaaaact ggcgaaccag ccaggcgtac acatctacaa cctcggcgct ggcgtaggca
    29401 acagcgtgct ggacgtggtt aatgccttca gcaaagcctg cggcaaaccg gttaattatc
    29461 attttgcacc gcgtcgcgag ggcgaccttc cggcctactg ggcggacgcc agcaaagccg
    29521 accgtgaact gaactggcgc gtaacgcgca cactcgatga aatggcgcag gacacctggc
    29581 actggcagtc acgccatcca cagggatatc ccgattaagg aacgaccatg acgcaattta
    29641 atcccgttga tcatccacat cgccgctaca acccgctcac cgggcaatgg attctggttt
    29701 caccgcaccg cgctaagcgc ccctggcagg gggcgcagga aacgccagcc aaacaggtgt
    29761 tacctgcgca cgatccagat tgcttcctct gcgcaggtaa tgtgcgggtg acaggcgata
    29821 aaaaccccga ttacaccggg acttacgttt tcactaatga ctttgcggcc ttgatgtctg
    29881 acacgccaga tgcgccagaa agcaacgatc cgctgatgcg ttgccagagc gcgcgcggta
    29941 ccagccgggt gatctgcttt tcaccggatc acagtaaaac gctgccagag ctcagtgttg
    30001 cggcattgac ggaaatcgtc aaaacctggc aggaacaaac cgcagaactg gggaaaacat
    30061 acccatgggt gcaggtcttt gaaaacaaag gcgcggcgat gggctgctct aacccgcatc
    30121 cgcacggaca gatttgggca aatagcttcc tgcctaacga aactgagcgc gaagaccgcc
    30181 tgcaaaaaga atattttgcc gagcagaaat caccaatgct ggtggattat gttcagcgcg
    30241 agctggcaga cggtagccgt accgttgtcg aaaccgaaca ctggttagcc gtagtgcctt
    30301 actgggctgc ctggccgttc gaaacgctac tgctgcccaa agcccacgtt ttacggatca
    30361 ccgatttgac cgacgcccag cgcagcgatc tggcgctggc gttgaaaaag ctgaccagtc
    30421 gttatgacaa cctcttccag tgctccttcc cctactctat gggctggcac ggcgcgccat
    30481 ttaatggcga agagaatcaa cactggcagc tgcacgcgca cttttatccg cctctgctgc
    30541 gctccgccac cgtacgtaaa tttatggttg gttatgaaat gctggcagag acccagcgag
    30601 acctgaccgc agaacaggca gcagagcgtt tgcgcgcagt cagcgatatc cattttcgcg
    30661 aatccggagt gtaagaaatg agtctgaaag aaaaaacaca atctctgttt gccaacgcat
    30721 ttggctaccc tgccactcac accattcagg cgcctggccg cgtgaatctg attggcgaac
    30781 acaccgacta caacgacggt ttcgttctgc cctgcgcgat tgattatcaa accgtgatca
    30841 gctgtgcacc acgcgatgac cgtaaagttc gcgtgatggc agccgattat gaaaatcagc
    30901 tcgacgagtt ttccctcaat gcgcccattg tcgcgcatga aaactatcaa tgggcgaact
    30961 acgttcgtgg cgtggtgaaa catctgcaac tgcgtaacaa cagcttcggc ggcgtggaca
    31021 tggtgatcag cggcaatgtg ccgcagggtg ccgggttaag ttcttccgct tcactggaag
    31081 tcgcggtcgg aaccgtattg cagcagcttt atcatctgcc gctggacggc gcacaaatcg
    31141 cgcttaacgg tcaggaagca gaaaaccagt ttgttggctg taactgcggg atcatggatc
    31201 agctaatttc cgcactcggc aagaaagatc atgccttgct gattgactgc cgctcactgg
    31261 ggactaaagc agtttccatg ccgaaaggtg tggctgtcgt catcattaac agtaacttca
    31321 aacgtaccct ggttggcagc gaatacaaca cccgtcgtga acagtgcgaa accggtgcgc
    31381 gtttcttcca gcagccggcg ctgcgcgatg tcaccattga agagttcaat gctgttgcgc
    31441 atgaactgga cccgatcgtg gcgaaacgcg tgcgtcatat cctgactgaa aacgcccgca
    31501 ccgttgaagc tgccagcgcg ctggagcaag gcgatctgaa acgtatgggc gagttgatgg
    31561 cggagtctca tgcctctatg cgcgatgatt tcgaaatcac cgtgccgcaa attgacactc
    31621 tggtagaaat cgtcaaagct gtgattggcg acaaaggtgg cgtacgcatg accggcggcg
    31681 gatttggcgg ctgtatcgtc gcgctgatcc cggaagagct ggtgcctgcc gtacagcaag
    31741 ctgtcgctga acaatatgaa gcaaaaacag gtattaaaga gactttttac gtttgtaaac
    31801 catcacaagg agcaggacag tgctgaacga aactcccgca ctggcacccg atggtcagcc
    31861 gtaccgactg ttaactttgc gtaacaacgc agggatggta gtcacgctga tggactgggg
    31921 tgcgacttta ctttcagctc gtattccgct ttccgatggc agcgtccgcg aggcgctgct
    31981 cggctgtgcc agcccggaat gctatcagga tcaggctgcg tttctggggg cctctattgg
    32041 tcgttatgcc aaccgcatcg ccaatagccg ttataccttt gacggtgaaa ccgtgacgct
    32101 ttcgccaagt cagggcgtta accagctgca cggcggcccg gaagggttcg acaaacgtcg
    32161 ctggcagatt gtgaaccaga acgatcgtca ggtgctgttt gccctgagtt cagatgatgg
    32221 cgatcagggc ttcccgggta atctcggcgc gacggtgcaa tatcgtctga ccgacgataa
    32281 ccgtatctct attacttatc gcgccacagt tgataaacct tgcccggtga atatgactaa
    32341 tcacgtctat ttcaatcttg acggcgagca gtctgacgtg cgcaatcaca agttgcagat
    32401 tctggcggac gaatatctgc cggttgatga aggcggcatt ccgcacgacg gcctgaaatc
    32461 tgtcgccgga acgtcttttg atttccgtag cgccaaaatc atcgccagtg agtttcttgc
    32521 cgacgacgat cagcgcaaag tgaaaggtta cgatcacgca ttcttgttac aggccaaagg
    32581 cgatggcaag aaagtggcgg cgcatgtctg gtcagcagat gaaaaattgc agctgaaggt
    32641 ctacaccacc gctccggctc tgcaattcta ctccggcaac ttcctcggcg gcacgccatc
    32701 aagaggaact gaaccttacg ccgactggca aggcctggca ctggaaagcg agtttctgcc
    32761 ggacagccca aaccaccctg aatggccgca accggactgc ttcctgcgtc ctggcgaaga
    32821 gtattccagc ctgacggaat atcagtttat tgctgagtag cacctgaagc cctcgtaatg
    32881 agggctttat ccttatattg ctcaaatcac cagcaaacac cgacatattt gcaactcaat
    32941 attcacaaca accttacact gcgacactat tttcgctatg gttatgcgta agcattgctg
    33001 ttgcttcgtc acggcaatat aatgagaatt attatcatta aaaaatgatt tgaggagtaa
    33061 gtatatggct gtaactaagc tggttctggt tcgtcatggc gaaagtcagt ggaacaaaga
    33121 aaaccgtttc accggttggt acgacgtgga tctgtctgag aaaggcgtaa gcgaagcaaa
    33181 agcagcaggt aagctgctga aagaggaagg ttacagcttt gactttgctt acacctctgt
    33241 gctgaaacgc gctatccata ccctgtggaa tgtgctggac gaactggatc aggcatggct
    33301 gcccgttgag aaatcctgga aactgaacga acgtcactac ggtgcgttgc agggtctgaa
    33361 caaagcggaa actgctgaaa agtatggcga cgagcaggtg aaacagtggc gtcgtggttt
    33421 tgcagtgact ccgccggaac tgactaaaga tgatgagcgt tatccgggtc acgatccgcg
    33481 ttacgcgaaa ctgagcgaga aagaactgcc gctgacggaa agcctggcgc tgaccattga
    33541 ccgcgtgatc ccttactgga atgaaactat tctaccgcgt atgaagagcg gtgagcgcgt
    33601 aatcatcgcc gcacacggta actccctgcg tgcgctggtg aaatatcttg ataacatgag
    33661 cgaagaagag attcttgagc tgaatatccc gactggcgtg ccgctggtgt atgagttcga
    33721 cgagaatttc aaaccgctga aacgctatta tctgggtaat gctgacgaga tcgcagcgaa
    33781 agcagcggcg gttgcaaacc agggtaaagc gaagtaaacg tcattcgttt aaaatgagaa
    33841 agccgactgc aagtgagtcg gcttttttgt tgctaacaat ggagcacatt gcctgatgcg
    33901 acgctgcgcg acttatcagg cctgtggtga ttcatcggat acgccactct gacggcgcat
    33961 ccgacaatta aaccttaccc gcgacgcgct tttactgcat tcgccagttg acgtaacaga
    34021 gcatgggtat cttcccagcc gatgcaggca tcggtgatgc tcttaccgta ggccagcggc
    34081 tccccgctct cgaggctctg attgccttcc accagatggc tttccaccat cacgccaata
    34141 atggcctttt cgccaccggc aatctgctgg caaacgtcag cacaaacatc catctgcttt
    34201 ttgaattgtt tggacgagtt agcatggctg aaatcgatca tcacctgtgc tggcaggcct
    34261 gctttgttca gcccttcttt cacttcagca acgtgcttcg cgctgtagtt aggctcttta
    34321 ccgccgcgca gaatgatatg gcaatcaccg ttaccgctgg tattcacaat cgccgaatgc
    34381 ccccatttcg ttacggacag gaagcagtgc ggcgcaccgg cggcattaat agcatcgata
    34441 gccactttaa tcgtaccgtc ggtgccattt ttgaagccga ccggacaaga aagccctgat
    34501 gccagttcgc ggtgcacctg cgattcggtg gtacgtgcgc caattgcgcc ccagctcatc
    34561 aggtcagcga gatattgtgg ggtgatcata tcgagaaact cacccgccgc tggcagaccg
    34621 ctgtcgttaa tatcaagcag caatttacgg gctatacgca gaccgtcgtt gatctggaag
    34681 ctgttatcca tatgcggatc gttaatcagc cctttccagc ccaccgtggt acgcggcttt
    34741 tcaaaataga cgcgcattac gatttccagc tcatctttca gctcttcacg cagcgccagc
    34801 aagcgagtgg catactcttt tgctgcgaca ggatcatgaa ttgagcatgg gccaatcacc
    34861 accaacaggc gatcatcatt acctttcagg atcttatgga tcgcttttcg ggcatgggca
    34921 accgtattcg cggcattttc agtagcgggg aatttttcca gcaatgcgac aggaggaagt
    34981 aactctttga tttctttgat gcgtaaatcg tcgttctgat aattcatgtc tgttccagtg
    35041 ttgccatact tatcttagtg aatgcaatac ttccaatcta tatcttccgt cagaatgtgt
    35101 aaacggggtt ttacactatg aacggattaa tcctggaatt agcaagaaaa acgccagatt
    35161 gtcgcgaaaa acgagatctc tcctacaatt tctaactgta actcctttgt ttgttaatta
    35221 tttcaagatt ctctgctgcg tttcataacc tggctgaaat cttaaaccaa tgccttatat
    35281 tcacctgcaa atgcactgtt ggaagaggtt atccgacata acgaccataa caggagcatc
    35341 ctatgaaaat gacaaaactg gccacattat ttctgactgc cactctaagc cttgccagcg
    35401 gtgccgcact ggctgccgat agcggagcgc aaactaataa cggccaggca aacgccgcag
    35461 ctgatgcggg ccaggtagcc cctgacgccc gtgaaaatgt cgcgccaaat aacgtcgaca
    35521 ataacggggt aaataccggt tctggtggca caatgctgca ttcggatggt tcttcaatga
    35581 acaatgacga aatgaccaaa gatgaagagc acaagaacac catgtgcaaa gatggtcgct
    35641 gtccggatat caataaaaag gtacaaaccg gtgatggcat caataatgat gtcgatacca
    35701 aaaccgacgg taccacgcag taaccctgca aaatagccaa taatgggaga gcggagtctc
    35761 tcccttttaa tttttcactt ctaaaagcag tatgatgtca taaagttaag tgagaataat
    35821 taagggatga ctcgtcatgg cgcactcaca ctcacacacg tcctcacacc tgccagaaga
    35881 taataatgct cgtcgcttgt tgtatgcttt cggcgtgact gccgggttta tgctggtaga
    35941 agtcgttggt ggttttcttt ctggttctct ggcattgctg gccgatgcgg gtcatatgtt
    36001 gaccgatact gccgccctgc ttttcgccct actcgccgtg caattttcac gtcgtcctcc
    36061 caccattcgc cacactttcg gttggctaag actgaccacg ctcgcggcgt ttgtgaatgc
    36121 catagccctc gtggtaatta ccattttgat tgtctgggag gcgatagaac gcttccgcac
    36181 gccgcgtccg gtcgagggcg gcatgatgat ggcaattgcc gtggctgggt tgctggcaaa
    36241 tatactttct ttctggttac ttcatcacgg cagcgaagag aaaaacctca acgtacgagc
    36301 agcggcactg catgtgctcg gggacctgct gggttcggtt ggggctatta ttgccgcgtt
    36361 gattattatc tggaccggct ggacacctgc tgaccccatt ctctcaatac tggtatcgct
    36421 tctggttctg cgtagcgcgt ggcgactgtt gaaagatagc gtgaatgaat tacttgaggg
    36481 tgcaccggta tcgctggata tcgctgaact gaagcgtcgt atgtgccggg aaatcccgga
    36541 agtccgcaat gtgcaccatg tgcatgtatg gatggtaggc gagaagccgg tgatgacgtt
    36601 gcatgtgcag gtgatcccgc cacacgatca cgatgccttg ttggatcaga tccaacacta
    36661 cctgatggat cactatcaga ttgagcacgc caccattcag atggaatatc aaccttgtca
    36721 tgggccagac tgccacctga atgaaggggt gtctggccat tcacatcatc accattaatg
    36781 ggacagcgcg cgtgagcctc tttcacgtgc gctgttgatc cacatccggc tgccgttgag
    36841 cgcaataaag gtcaggatga tgtactccag agacatggcg taaacgccct gaagtgcaaa
    36901 aataacgacg ctaatcacgt taataatcac ccacaacagc cagttttcca catacttacg
    36961 cgtcatcaga atcattgcca cgatagataa caccatcatg caggaatccc agaacgggaa
    37021 agcgtccggt tgcagttcag gcatcacaac ctgtaatcct aatgcttgca tgatcatgac
    37081 tgccacgcgg gtcaaaaatg caaacaccgg attgataaat accgtcatca gaccaatcga
    37141 aacaacgcaa accgccaacc agctgagcgc cttcggcaat ggtaaccagc gaattttcaa
    37201 ctccgcctcg ttctgactgg tttgtcgcga ccacgcatac caaccgtaaa tattcgcggc
    37261 aaagaaaaac acctgtaata gcaggctggc atacagctga atctgaaaga aaataatgcc
    37321 aaacaaggtg acgttgatca ggccaaagaa gtagttgctg atcttctcca gactggcaag
    37381 gccaatacac agcaacccgg cgatcgtgcc taccgcttcg atccatgaga gatcataacc
    37441 gcctgcccct attggtatat gtaccaggat attctgcaca ctaaaaaaat ccatctttcc
    37501 cccaaagcgt aacgttacgc cttattattc gttatccacg tagtgtagcc gcaaaatcca
    37561 gcatacgatt gagcggcacc agcgccctct ctcgcagcct ttcatcaaca tgaacctcgt
    37621 gattgcttcc ttcctgttct aatgcctctg cgatagcctg aaggccattc atggccatcc
    37681 acggacaatg cgcgcagctg cggcaggttg cgccctcacc cgcggttggt gcttccagta
    37741 actctttatc tggcaccgcc tgctgcattt tgtagaaaat gccccgatcg gttgccacga
    37801 taagccgctg atgtggcaat gtttttgcag cagcgatcag ttgactggtg gaaccgaccg
    37861 catccgccat atcgacaata gcttgtggtg attctggatg caccagtatg gcagcatccg
    37921 ggtattcttc ttgcaagcgg gttaacgcct gagtcttaaa ttcatcatgc acaatgcagg
    37981 caccctgcca gcataaaatg tctgcgcccg tctgtttttg cacgtaacgc cccagatgtt
    38041 tgtcgggtgc ccagatgatt ttttcaccca aactatcaag atgatcaata agttcgacgg
    38101 caatgcttga agttaccacc caatctgcgc gcgcttttac cgcagcagaa gtgttggcgt
    38161 agacgacgac agtacgatct ggatgggcat cgcaaaatgc gttaaattct tcaacagggc
    38221 agccgagatc cagtgaacac tcagcctgaa gtgtcggcat cagaattgtt ttttccggac
    38281 tgagaatttt ggcggtttct cccataaatc tcaccccagc gactaacaac gtagaagcgg
    38341 gatgctttgc accgaagcgc gccatttcca gagaatcaga aatacagcca ccggtttctt
    38401 ctgccagttg ttgaatttcg gggtcggtat agtagtgggc aaccatcacc gcattacgtt
    38461 cttttagcag acgttttatc ttctcgcggt aatacgcttt ttcatcaatg cttaacggcg
    38521 tcggcttcgg ggggaaagga taaatcgccg tgtctggatc aaacatcacg ctcatcttac
    38581 catctcgttt tacaggctta acgttaaaac cgacattagc gtaatattcg ctgttatcgg
    38641 tgtaatttgt tttatatgct aaacaagata gtcgaatggt ggggaaaagt cactcgaatt
    38701 acgtatgaga tgccgaatgc cacactttgc atcttattcc tccgacgtag agttgtttac
    38761 gcagcaagcg cggtatatgt catcagggtc aagaattgaa attgagagat gcctgaatag
    38821 caaaaaagcg cctttagggc gcttttttac attggtgggt cgtgcaggat tcgaacctgc
    38881 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccct tgcgggatta
    38941 tgctgcttta atcattcagg atggtgggtc gtgcaggatg actcgacttc gtctcgccct
    39001 tcgggccgtt gctaacgcaa cgttatcctt cacgttctct accagtaacc acctttaaat
    39061 tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca
    39121 actgagctaa cgacccatat gggtgttacc tgaaagattt ctactcggaa ccaatcttta
    39181 aaattggtgg gtcgtgcagg atgactcgct tcgctcgccc tacgggccgt tgctggcgca
    39241 acgttatcct tcacgcttaa catctgagtt tgatgttaaa actggtgggt cgtgcaggat
    39301 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccga
    39361 gtggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc
    39421 agctgagcta atcacccgat actgtgctgg atactacaaa ctacttccac cgaacccacc
    39481 gcaattagtg gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg
    39541 ctctaccaac tgagctaacg acccggtggt gggtgatgac gggatcgaac cgccgacccc
    39601 ctccttgtaa gggaggtgct ctcccagctg agctaatcac ccgatactac gctggatact
    39661 acaactaact atgttagtgg tgggtcgtgc aggatgactc gacttcttct cgcccttcgg
    39721 cgccgttgct gatgcaacgt tatccttcac gttttactac cgcttccacc ttagcgattg
    39781 gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg ctctaccaac
    39841 tgagctaacg acccactttt tcgttgcttt cggtttgttt gatatcccgg ggcaacggcg
    39901 gcatatatta ctgatttcag acttgagcgc aacaaaaatt tcgatgtaga tcactcaact
    39961 gcttatgatt cgcacgacac gaccagaaat aatgcgactt ctggtcgtgt gttatgcatt
    40021 acatcgcgtt cagacgtttt tgtgcctgtt tagcgccatc agtaccaggg tatttactga
    40081 taacctgctg gtatacggct ttcgcttttg cggtgtcacc tttgtcctgc atgatgacgc
    40141 cgactttaaa catcgcatct gcagcctttg gtgacttcgg atagtttttc actaccgaag
    40201 caaaatagta cgccgcatca tcttttttac ccttgttgta gtttaactga cccagccaat
    40261 aattggcgtt tggcaggtaa gttgaatcag ggtaattttt gatgaaattc tgaaatgcca
    40321 ccattgcgtc atcctggcgg gatttatcct gcaccagcgc aatagctgca ttgtaatccg
    40381 tgtttgcatc gccgcttttc accggcgcgc cagcattcgc agtaccagca tcagctgtcg
    40441 gcgtcgttga tgccgccgca ccgctttgat cgctgctggt tgattgcgcc gctgcaccac
    40501 cgctgctgag gctgtcgatc tgcaacagga tctgcttttg ccgctccacg acctgattca
    40561 gttgatactg attttcctga atctgaccac gcagggaatc aatatcggat tgattatcag
    40621 agagttgttg ctggagttgg gttaaaagct ggctgtgagc attagaaata cgctcaagtt
    40681 gagtgacgcg gtcttcgacc gagcctgagc cgacactact gattggtgcc tgagcaaaag
    40741 cggcccaggg ggccgctata ccaaccagta acgacagact caatagttga tgtctgaagt
    40801 tactgctcat gcaattctct tagtaaacca gtaccgcacg acggtttttg gagtatgccg
    40861 cttcgtcatg acccagtact gcaggttttt ctttaccgta agaaacgatg gagatctggt
    40921 ctgcagaaac gcctttaccc tgcaggtaca tcttaacggc attcgcacga cgttcaccca
    40981 gggagatgtt gtattccgga gtaccacgtt cgtccgcgtg accttctacg gtgactttgt
    41041 aagacgggtt gctacgcagg aagtttgcat gtgcatccag catttgagcg aagtcagaac
    41101 ggatatcgta cttgtccaga tcgaagtaaa cgatgttgtt ctgctgcagc tgttgcattt
    41161 gcagacgagc ctgctcttcg gaagacatgt tgccgttgcc gccgttcgca tccataccag
    41221 tgccggcacc cagcatgcct tcgctgccgt cattgctggc gttcttgttg gaagaacatg
    41281 ccgcaattgc cataacaggc agagcaatca tcagcccttt cagcactttg ttcagttgca
    41341 tttcaatgat tcctttacta ttcaattaat tattatcaca gatacggcga ccaggcaggg
    41401 aatttgacct gtccatcagt tgccggaaga cgcgctttga aacgcccatc tgtagaaacc
    41461 aaattcagca cggatcccat cccctgagaa gagctgtaga ttaccatagt gccgttaggt
    41521 gccagacttg gcgtttcatc caggaacgtg gacgacagaa cttgtacgcc tcccgttgcc
    41581 agatcttgtt tggcaatgtg ctgctgccca ccgttggagc tgaccattac cataaattta
    41641 ccgtcgctgc tgacatccgc atcctggttc tgcgaacctt cccaggtaat acgttgtggc
    41701 gcaccgccgt tgatattcac tttataaacc tgtggacgac cggcctggtc agaagtaaat
    41761 gccaggttct ggctgtccgg gaaccaggtc ggttcggtat tgttactgcg accatcagtc
    41821 acctggcgga tctgaccaga agccaaatcc attacgtaca ggttcaggct accggttttc
    41881 gacaaggcga atgccagttt gctgccgtct ggcgagaatg caggcgcacc gttgtgacgc
    41941 gggaatgaag ccacctgacg tacagcgccg tttgccagcg tttggataac cagcgcggaa
    42001 cgaccgcttt cgaaggtcac ataagccagt ttagaaccgt ctggtgacca tgccggagac
    42061 atcagcggct gcggtgaacg gtgaacgaca aactggttgt aaccgtcata gtcagatacg
    42121 cgcagttcat acgggaactg accgccgttg gtctgaacaa cgtaggcaat acgggtacgg
    42181 aacgcacctt taatgccggt cagtttttca aacacttcat cactggcggt atgaccagca
    42241 taacgcagcc actgcttgtt cactttgtac gagttctgag caagtacagt acccggtgcg
    42301 ccgccagtgt caacaagttg ataagcaaca ttgtaagaac catccggatt cggagtgacc
    42361 tggccgacaa ccacagcgtc aatgcccagc gcggaccatg cagctggttg tacttcctgc
    42421 gcactacccg gctgctgtgg cagacgagcg cgatctaacg gattaaattt accgctgtta
    42481 cgcaagtcag cagcaacgat gccgccaata tcttcaggtg ccgcaccagg acccgcccac
    42541 tggaaaggaa caacaccaat aggacgaccg gaatctacac cgctgtcgat cacaatgcgg
    42601 acttcagcat gcagaactga tgcccacagt atgagaaaac caaatgctac tcgtaatgcc
    42661 tgcttcatca tatctccctt atctggacca atggcccacg ataatttagc agaatgttaa
    42721 caaactaaaa tacacaaaac taccagaacc ccgtggcaac cggtgcctga tgttgaccgt
    42781 cggaacagtc aacatcgcga ttacggtttg aagtccaatg gcgcgttttt gaacacttca
    42841 tatactgcct ggcttggtgg tttcgggatc ttcgcaagtt tagctgctgc caacgcagct
    42901 tgacaaagtg cgggatcgcc accttcaggt ttgatatcca gtaacatacc atcgggtgcc
    42961 agttttatgc gcagcgtaca ggttttgcct gcataggacg atgcgtcata gaacttactt
    43021 tcgatagcag atttaatctg cccggcatag ttattgatat cggcccctga tgcgccattg
    43081 tttttagtat taccactccc ggcaggcgaa gcattgttcc ctttcgcccc tccccccgtt
    43141 ttcggtgcat tcttaccaga gcttagctca ccgaaaatat catctgcctc tgcggcagct
    43201 tttgctgcag cggctttttc agctgctgct tttgcagctg ccgctttttt gtctgcggcg
    43261 gctttttctg ccgctgcttt cttatcagct gctgcctttt cagcagccgc tttttctgct
    43321 gcttttttgt cggctgcagc tttctctgct gccgctttct tatcagctgc agccttttca
    43381 gcagccgctt tcttctcagc ttctgccttg gctttttcag cagcttcagt tgccgctttc
    43441 tttcttgctt cagctgcagc tgcttctgcc gcttccgctt tcttcttcag cgccgcagcg
    43501 gctgcctcgg cttttttctg cgcttcggct gcggctttgg cggcttctgc ttctgctttt
    43561 ttcttcgcgt ctgcagccgc tttcttcgct gcttcttccg cagctttagc atccgcttcg
    43621 gccttcgctt tagcatctgc cgccgctttc gctgccgcct cttccgcttg cttctgcttt
    43681 aactcggcct gttttgcggc ttcttcagcc tgctttttct gttcctgagc agctaaccgc
    43741 tctttctcaa gttgcttcag gcgttcctgt tcagccgctt gtttctcacg cagttcttca
    43801 gcagcctgct gttccttcat cttgcgctgc tcatcagaac gcttcgcgct tgattcctgg
    43861 ctttgcatgc gtttgtactg ctcaactacc gcacctgaat caaccatgac agcgtcgatg
    43921 gacgaaccac cgccgcctcc tgctgaagct tctatattct catcgaacga actccagatc
    43981 agcgccgcaa ataagatgac atgcagcact gctgaaataa ttatcgcccg cttgagcttg
    44041 tcgttttgtt cggttgcctt tgacactctc ggtttccaaa aactgttcgc ctgttacccg
    44101 ctctctttca agcaagggaa acgcagatgt ttagataggc tgcgtcatta aaccaaccga
    44161 tttcacaccc gcactatgta acaagttcag tgctttaatt atttcatcgt aaggcacatc
    44221 ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc tggacacttc
    44281 cgccaccacc tgctctggtg gtaaacgctc caggcgatct ttctcaacca ccacggagta
    44341 ctgaccaata ccagacactt caacaatcac tggcggatta tcgttactgc tcaccgcctg
    44401 tgattcagta gcgtctggca gatcgacctc cacgctctgg gtgatgatgg gcgctgtcgc
    44461 cataaagatc agcaacagca ccagcagtac gtccagcaac ggtacaatgt tgatttcgga
    44521 cttgagatcg cgacgacctc gtccacgcgc tctggccatg gcttacccct tgttgctctc
    44581 gctaacggta aacgcctggc ggtgcagaat cgcggtaaac tcttccataa agttgtcgta
    44641 attcagttcc agtttgttta cgcgttggtt gagacggtta taagccatta ccgccggaat
    44701 agcggcgaac agaccgattg ctgtagcaat caacgcttct gcgatgcctg gcgcaaccat
    44761 ttgcagtgtt gcttgtttta ccgccccgag ggcgataaag gcgtgcatga tcccccagac
    44821 cgtaccaaac agaccaatat atgggctgat ggagccaacc gtaccgagga acggaatgtg
    44881 cgtttccaga ttttcaagtt cacggttcat ggaaatacgc atcgcacgcg acgccccttc
    44941 tactacggct tccggcgcat ggctattggc acgatgcagg cgcacaaatt ctttgaaccc
    45001 gctgtaaaag atttgttccg aaccggtcag attatcccgt ttcccctggc tctcttgata
    45061 gaggcgagag agttcgattc cagaccagaa tttatcttca aacgcttcgg cttcacgcgc
    45121 cgctgcgtta agaatacggg ttcgctggat aataatggcc caggatgcga ttgaaaaacc
    45181 aatcaaaatc aacatgataa gtttaaccag aaggctagcc ttcaggaaca aatcaaggat
    45241 attcatgtca gtcactgctt aaactccgcg acaatagact tgggaagcgc acgaggcttc
    45301 attttgagtg ggtcaacgca aacaaccaga acctctgctt cattcagcaa agtattctcg
    45361 gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac gcattgatgt tatttcagtc
    45421 tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt cattttacgt
    45481 accacaaagg caacgcgttc agccatcagc gcctgctgac tgaagtgatg atgacgcagc
    45541 atctctgtgc gtgctctttc ataaaaagcg acgtaactgg cgtggtacac cacaccaccg
    45601 gcatcggtat cttcatagta gacgcgaacc ggccatcgaa acagcgttgt attcacttta
    45661 catcccggta atgcaacaaa agttagagct tttaaacgct gctactatac gcgcgcgatt
    45721 gaggtttggg aatgagggca agttaaggga gcgtaaaaat ttatgggcct ggagcggccc
    45781 ataatttttt aaagagaatt ctgatttaaa agaagaagaa aatcagcccg acaatgagaa
    45841 caatatcggc aagcaatggg caaaaaatcc cttgccaaag aaccttctgc ggacgaaagc
    45901 ctacgccatg aatcacacca gcacagacgg cccacatcag caataaacca tgccagattt
    45961 ccagttcact ggtcttcgcg gcaaaacgtg acgggtccca aaacatacag cctgctaaca
    46021 gaagcgccat cacgaaggaa agcgcccgca gggggcgctt atccattacc gcatacaagg
    46081 ttacgataat cttactcatc agatgtcttc ttgaccggct ttgcctgatt cgacgtgttc
    46141 aagcgccagc gcggtgatta ccccaaacga acaggcaaga agcgttccca gaatccatgc
    46201 gaaataccac atttttagct ccttacttag tacagagagt gggtgttacg ttcaatatct
    46261 tctttggtga tacgaccgaa cattttccag taacaccagg cggtgtagag cagaatgatc
    46321 ggtaccagaa ccaccgcaac ccaggtcatg acgttaagcg tcagctggct ggaagttgca
    46381 tcccacattg tcagacttgc gttcatcatg gtgctggacg gcatcacaaa cgggaacatt
    46441 gcgataccgg ctgtcaggat gatgcaggcc agcgtcaggg aggagaacac aaacgcccac
    46501 gcggctttat ccatacgtgc agtcaggatg gtcagcagcg gcagaaccac acccagtgcc
    46561 ggaatagccc acagaattgg cgtgttgttg aagttaacca gccatgcgcc agcttcacga
    46621 accacttctt tattcagtgg gttagaggct gcgtaatggt ccattgtcga tttcacgaca
    46681 taaccatcga taccgtacat cacccatacg ccagccagtg cgaaacagac cagtgtcacc
    46741 agcgcagcca cctgagccgt tgcacgggta cgcaggtgca gttcgcccac ggtacgcatt
    46801 tgcagatagg ttgcgccctg agtaatgatc atccctacgc tcaccacgcc tgccagcagg
    46861 ccgaacgggt taagcaactg gaagaagtta ccggtgtagt acagacgcag atattcatca
    46921 acgttgaacg gtacgccctg caacaggtta ccgaacgcta caccaattac cagcggcgga
    46981 acgaagctac caatgaagat gccccagtcc cacatgttac gccagcgggt ttcttcaatc
    47041 ttggagcggt agtcaaaacc gaccggacgg aagaacaaag acgccagcac gaggatcatc
    47101 gccacataga agccggagaa cgcagcggca tagaccatcg gccaggcagc aaagagtgcg
    47161 ccgcccgcgg tgatcagcca aacctggtta ccgtcccagt gtggtgcaat ggagttaatc
    47221 ataattcgac gctcggtgtc gttacgaccg aggaaacggg tgagcatgcc cacccccatg
    47281 tcgaaaccgt cagtgactgc aaaaccaatc agcagaacgc caaccagcag ccaccagata
    47341 aaacgcaata cttcataatc gatcatttga cgactcctgt cttagcgtgc cggctgagta
    47401 gtcgtggaag actgctcaaa gtgatagcga ccggttttca ggctgcttgg gccgaggcgt
    47461 gcaaacttga acattaagaa caattctgcc accaggaaca gggtatacag gccgcaaatc
    47521 agcaccattg agaagatgag atcgcctgcg gtcagtgacg agttcgccac agctgtcggc
    47581 agcacttcac cgatagccca cggttggcgg ccatattcag ccacgaacca gcccgcttct
    47641 acagcaatcc acggcagcgg aataccgtac agcgcggcgc gcagcagcca ttttttctcg
    47701 ccaatgcggt tgcggatgac actccagaaa gagagcgcga tgattgccag cagcaggaag
    47761 ccacacgcca ccataatacg gaacgcgaag tacagcggcg ctacacgcgg aatggagtct
    47821 ttggttgcct gttgaatctg cgcttcagtc gcatcagcca cgtttggcgt atagcgtttc
    47881 agcagcagac cgtaaccgag gtctttcttc atgctattga actggtcacg aaccgcctgg
    47941 tcggtagaac cagaacgcag ttgttcgagc agagagtacg ccttcatccc gttacgaatg
    48001 cgttcttcat gctgcaccat cagctctttc aggccgataa ccggggtatc cacggaacgc
    48061 gttgcaatga tgcccagtgc gtaagggatc tgaatcgcaa atttgttcgt ctcctcttcc
    48121 tgatcaggaa tgccgaacag agtaaaggca gcaggcgcag gttgcgtttc ccactcggct
    48181 tcaatagcag ccagtttggt tttctgcacg tcgcccattt cgtagccgga ttcatcaccc
    48241 agaacaataa cagacagaac agcagccata ccgaagctgg cagcgatagc aaaggagcgt
    48301 ttagcgaagg cgaagtcacg acctttcagc atataccatg cgctgatacc gaggatgaac
    48361 atcgcgccag tcacataacc agacgctaca gtgtgaacga atttcacctg agcaaccggg
    48421 ttaagcacca gctcggagaa gctcaccatc tccatacgca tagtttcaaa gttgaaatcg
    48481 gacgcgattg ggttttgcat ccagccgttc gcaaccagaa tccacagtgc ggacaggttt
    48541 gaaccgagcg ccaccagcca ggtgacacac atatgctgaa ctttacccag acgatcccaa
    48601 ccgaagaaga acagacctac aaaggtggat tcgaggaaga aggccatcag accttcgatt
    48661 gccagcggcg caccgaagat atcccctaca tagtgggaat agtaagacca gttagtcccg
    48721 aactggaact ccatggtcag accggtagcc acacccagag cgaagttgat accaaacaac
    48781 ttgccccaga acttggtcat atctttataa atctgtttgc cggagaggac gtagaccgtt
    48841 tccataatgg ccagcaggaa cgccataccg agcgtcagtg gcacaaaaag gaagtggtac
    48901 atcgcggtca aggcaaactg taagcgcgac agttcgacta tatctaacat catgactcct
    48961 tgctcatcgc atgaagactc cgagagtgaa ccccgttaga aagggtcaca cgcatgcccc
    49021 aatagaaatt tgtttgaaca ctttcatcat accatgtgaa tgcacacaaa aacggcgtaa
    49081 agaaaggttg caaacatgtt aataaaaact caaattgatc ccacgtatat attacgccgc
    49141 aaaatcctta caataaacag gtttttgttg aagcaaattt gcatttttcg acggtgatca
    49201 atttatagct aaattaccgc ctttcagcca atttgatcga gaacaattta tctctttttg
    49261 atgcccattt ccaagactta tacattgata aatatcaatt tctacaaatt atgttaatca
    49321 tgtttcaata aggtaacttt agtgacattt atgtttaaaa tgtgtgagtt ataaggctga
    49381 aattagtttc ggttggcaat taaagataaa aagagacgaa gcaaaaacag cacggttgac
    49441 cttaattctt tttttggtca ctggctaata cgacgcagag tttcaggatt actaataaag
    49501 gcataaatcc accccgccga tattaacatt taattaactt ataattatat tataattaac
    49561 tcacccaggg ttattatttt gttaaaaata aagccacata tacgtggctt gagagtgaag
    49621 ataaacgcaa aattacctga tgcgctacgc ttgccggtgt ctacatcatc tctgcaatat
    49681 attgaatttg cctgcttttg tagaccgaat aaagcgttca tgccgtatcc ggcatgaaca
    49741 aagcgccctt tgtcaacaat ctggccgcgc atatgtggct tcggtttgat tgctattcag
    49801 gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga tagatttgaa
    49861 ccttgatttt cctcggtggt aatgtgttca tccatcatcg tttctgagca agaaatcacc
    49921 tcgcgactga aagcaacagt cgcatcacag gttgctgatt cagccggatt aaacagccgt
    49981 aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca tcccactggg
    50041 ggcattttca acaaactata actttccggc acgttgaatc cggctttgtt gagcttcatc
    50101 gcatcccatg ggattttgtt gtaacactgt actggagtca gccatgctcg cgcctgctga
    50161 gctacaccag cggcggttgg cgtaccggta taactcaata aacttaggcg acaagaaagc
    50221 agaccacgta gttgtgagtc cgggactggc attttaattc ccgaaggccg cccaggcctt
    50281 aaaagcagat cttctttgcc cagtaagccc acgccacgca gcaacgtaat ggcaaaggtt
    50341 ttcttctctt caccgatgac ttcaaattca cgtaaccctt cgctaaagac agccatgccg
    50401 ttacgccctt cctgtaaggc aacatagttg agcatattcc ataccggaac cggcgcttct
    50461 ttccagcctt cttgctgcca gtcgttcatt gcactgtcgt tcacggggcg cgttagcgaa
    50521 ccaaactggg tatcggccag aacactgtcg gtgttaaaag gtgtagggac caggacgcga
    50581 aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatgcg cctgctgtta
    50641 tcactcagag tgacagccaa ctccacccct actctccccg tggattgccg gacgctgcgt
    50701 tcttgcagat tacgcggcac cgccatgtca tagcgaataa cagccctact ctgccaggcc
    50761 tcatgagtaa tctcgcattg cggtttcgca ttcgccgaag taattaccca ctcttctttt
    50821 gcaggtgaat agtcatattc atcaccatca tcagagcttt cttcaatttg caacacccgg
    50881 tcatagcgca caccgctgtc tttatctacc agtcgtagcg ttccatcctc attgagtgca
    50941 atttgccaga aagcattttc cagtatccct tcagcgtcac tttttgccgc aatcacgttg
    51001 ccaggctgat tcgcttcgat ataaagcgtg cgatagccca tagaagggac aatctggttg
    51061 atctgtatat caaactccat aaagggatcg taattaccgt aatgaacaat ttgccgatcg
    51121 attaggcctg gatcgatctc acgggcatgg cgaataaaat acggtacagg ctgaccgcga
    51181 tcgtcccgca aattaaactg gctggcgcgc agccgcacag tggtgttgat cacttcttcc
    51241 cgcggccagg gcatcaggtt aaacagcacg agtttgtcgg catcactctg tggcatgtta
    51301 tcggcaattt tgcgcatgta gaaacgcagt agattatccg ccatgtcttc agccagttcg
    51361 aagcgggcga cgatttcgcg atgaactttg tcactacagc agcagccgat gctgtcgtgg
    51421 gcatgatttt ttaagatctc tttccacatt ttctccagca agccgtggtg gtattcaaaa
    51481 cccaacgtcc aggccagcgt tgccagcggt tccagcagat taacaatctt attttcaata
    51541 cgtgcgtggg caactttgat atccatgcgc gtggaaccga tggtgcgatg cacgcgcata
    51601 tatttgccat caataaattc ccctttcagg gtcgccagac tttcacgctg cgcttcgatc
    51661 ttctcaaata cctcttcaaa gcggctcatc acaaacttac gttgagggta gatctcacgt
    51721 agcttatcca tcacttcgaa gatattttgc tgcaatggca tttggtcatg cccattcggc
    51781 agcaaaatct ctttggttac agacgctttt tccagcacgt caaaataact gtcgaggcgt
    51841 ttgcgtaatc cgtcttcgtc ggcaggtaag tacttaccga tggcataacc caacggcagc
    51901 acctgcgccg tcacttcact gccgtcactg ctttgccaca aaaactcggt tttatcagta
    51961 ccgtggcgct cagaacatcc acgccagaac atggtgcggg taatgccaaa tccattgtag
    52021 atatgcggca gttgcccgga catgccaaag gaatctggta aataacctat tttcatcggc
    52081 tcgccaaacg cgaggcagtc acgcattccg tacatcagat tacggacgat ggattccgca
    52141 gaaacaatcg tggtatcggt ctgggtatac caggggccga taatcaactt gccggcttct
    52201 accagtttct tcacgcggtc tttgttttcc ggtttcaccg cgaaataatc ttcgaggatc
    52261 gccgtttgcc cgtcgactac gtaatatttg tattcgttgt cctgttccag tcggcacagg
    52321 atctcttcca tattattgac cagcagaatg cgtgactctt cggtggtgaa ataccactcc
    52381 cgatcccaat gcatatgcgg ggtgatgtga acgcgagata ccgctttcat cgtcgtttcc
    52441 tttttgttta tggcattacg ccatcagtca gatagttgcc atgcttaacc gcgtgacgcc
    52501 gccagatcag gagaattgca gtcgagattg cagcccccac caatgccgca ccaaaccagc
    52561 caattgccgc cataacaccg cccgcgccat tatcatgaag taaaaagagc gagaaaatgc
    52621 cggcaccggg tgtcgaaagg ccgatattca tcgcaccgac aatagcgccc gttaccatag
    52681 agcccagcac aaacgaacca ataacccgca gcggatcttc aatcgccatc gggatcgccc
    52741 cttcggtaat acctgccagc cctaacagcc aggtggattt cccggtctca atttcaaact
    52801 ctttaaacag gcgcggtgcg agcatcgtgg aagcggttac ggtaaatgtc gaaaccattt
    52861 tgacggaggc gaaaatggca tacgggccgt aaacgccgtt cgccattgcg cccaggcaga
    52921 atgcataagc ggctttgttc actggccctc caaggtcaaa ggaacacata aaaccgagaa
    52981 tggcacccag caacagcgcg ttacttcctg acagaccgtt cagccaggcg gtaagcgagt
    53041 tattgatcca tgcgacaggt tctcccacca caaacagcat cagactgccc gctcccaacg
    53101 taccgagcac cgggtagaga taaaaagtca ggaatccatt gaatttactg ctaagacgca
    53161 agtgattttt cacccagcgc atcaagtaac cggctatcaa tccgccaacg accgcgccga
    53221 gaaacccgga gccgatcatg ttggcggcaa gtccagccgc aaagcctggc gctaacgccg
    53281 gtttatctgc cagagaatag gcggtatagg ccgcgagcac cggtaccatc aaaattccga
    53341 gcagcccgcc gcccagcttg cggtacatcc acagccagga gttttcttca ttaaacagat
    53401 cttgtagccc gaagatttgc gacagtaata ccgcgaccgc cagcaccgtg ccccccgcga
    53461 caatcaacgg tacggcaaaa gagattccgc tcaacagtgc ctgtttcagc tccgttttga
    53521 cacttttcac cggttgcgta tcttgctgta cggtacgcgt ctcatcgcta cgcttgaggg
    53581 taagcgcttg ttggatcaac gcttctgcat ggcgaatcgg ctcggcaaca ggcactgaaa
    53641 gcgcgggaat gccattaaaa cgctcactct ccttgatggc gacttcagcc gcaaaaatac
    53701 aggcggttgc actattgagt tgatccgccg ttaaacgccc ttcaatgccg ttagcgcctt
    53761 gtttttcaac gtaaacattt acgccgagtt tgcgtccggc tttttccaga tattccgcag
    53821 ccatataggt gtgagcaata cccgccggac aggccgttac gcagacgata gttggcgcgt
    53881 tggaaaaaga ggcagaaggt tgcgtgcctc ccttgtcatc cagcgccgag agcaactcat
    53941 caggcgtcgt cgccgactgt atacgcgccc gaatctcatc atccgcaagg cgcgtggtca
    54001 gcgctgtcag cagttgcata tgcgtagtac ccgcttcatt ggggggaatc gccagcagca
    54061 ccactaaatc aactgcttcc gggccatcaa cgccttccca ctgaagcggc tcgctgagtg
    54121 tggcgaccgc aaacgccgct tcttttaccg cagcagtttt gccatgcggc acagccaacc
    54181 cttcacctaa ggcggtcggg ccaaggcttt cacggcgata cacttcttcc agaaattgct
    54241 cagtactgga aattttcccc agagcagcaa gacgttgagt caacgcgtgg atggcctctt
    54301 cacggctggt aaagcgcgca ttcagacaca acgcatcgcg gtgggtcaga gtcgtcagat
    54361 tcatcatgat cctcacttga tcgtttttat agtcgcgcca gtgtgcccga taaaacaata
    54421 ccattatgtg atcaatttca cttgattgaa attatttgta ttgtatttgt attatttaca
//
LOCUS       DAOFQD010000017        53826 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.017, whole genome shotgun sequence.
ACCESSION   DAOFQD010000017 DAOFQD010000000
VERSION     DAOFQD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.017"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(125..2392)
                     /gene="adiA"
                     /locus_tag="QBF46_001073"
     CDS             complement(125..2392)
                     /gene="adiA"
                     /locus_tag="QBF46_001073"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDQ6673286.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2591..3499)
                     /gene="melR"
                     /locus_tag="QBF46_001074"
     CDS             complement(2591..3499)
                     /gene="melR"
                     /locus_tag="QBF46_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDQ6673287.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3782..5137
                     /gene="melA"
                     /locus_tag="QBF46_001075"
     CDS             3782..5137
                     /gene="melA"
                     /locus_tag="QBF46_001075"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDQ6673288.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5240..6661
                     /gene="melB"
                     /locus_tag="QBF46_001076"
     CDS             5240..6661
                     /gene="melB"
                     /locus_tag="QBF46_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDQ6673289.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGSALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6800..7429)
                     /locus_tag="QBF46_001077"
     CDS             complement(6800..7429)
                     /locus_tag="QBF46_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDQ6673290.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7552..9198)
                     /gene="fumB"
                     /locus_tag="QBF46_001078"
     CDS             complement(7552..9198)
                     /gene="fumB"
                     /locus_tag="QBF46_001078"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDQ6673291.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9276..10616)
                     /gene="dcuB"
                     /locus_tag="QBF46_001079"
     CDS             complement(9276..10616)
                     /gene="dcuB"
                     /locus_tag="QBF46_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDQ6673292.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11187..11906)
                     /gene="dcuR"
                     /locus_tag="QBF46_001080"
     CDS             complement(11187..11906)
                     /gene="dcuR"
                     /locus_tag="QBF46_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDQ6673293.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11903..13534)
                     /gene="dcuS"
                     /locus_tag="QBF46_001081"
     CDS             complement(11903..13534)
                     /gene="dcuS"
                     /locus_tag="QBF46_001081"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDQ6673294.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13715..13945
                     /locus_tag="QBF46_001082"
     CDS             13715..13945
                     /locus_tag="QBF46_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDQ6673295.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13957..14229
                     /locus_tag="QBF46_001083"
     CDS             13957..14229
                     /locus_tag="QBF46_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ6673296.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14456..14752
                     /gene="ghoS"
                     /locus_tag="QBF46_001084"
     CDS             14456..14752
                     /gene="ghoS"
                     /locus_tag="QBF46_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDQ6673297.1"
                     /translation="MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14780..14953
                     /gene="ghoT"
                     /locus_tag="QBF46_001085"
     CDS             14780..14953
                     /gene="ghoT"
                     /locus_tag="QBF46_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDQ6673298.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15072..16589)
                     /gene="lysS"
                     /locus_tag="QBF46_001086"
     CDS             complement(15072..16589)
                     /gene="lysS"
                     /locus_tag="QBF46_001086"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDQ6673299.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16826..18283)
                     /gene="dtpC"
                     /locus_tag="QBF46_001087"
     CDS             complement(16826..18283)
                     /gene="dtpC"
                     /locus_tag="QBF46_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDQ6673300.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(18342..20489)
                     /gene="cadA"
                     /locus_tag="QBF46_001088"
     CDS             complement(18342..20489)
                     /gene="cadA"
                     /locus_tag="QBF46_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDQ6673301.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20569..21903)
                     /gene="cadB"
                     /locus_tag="QBF46_001089"
     CDS             complement(20569..21903)
                     /gene="cadB"
                     /locus_tag="QBF46_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDQ6673302.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22269..23807)
                     /gene="cadC"
                     /locus_tag="QBF46_001090"
     CDS             complement(22269..23807)
                     /gene="cadC"
                     /locus_tag="QBF46_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDQ6673303.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24424..24499)
                     /locus_tag="QBF46_001091"
     tRNA            complement(24424..24499)
                     /locus_tag="QBF46_001091"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24464..24466),aa:Phe,seq:gaa)
     gene            complement(24606..25181)
                     /locus_tag="QBF46_001092"
     CDS             complement(24606..25181)
                     /locus_tag="QBF46_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6673304.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(25218..26915)
                     /gene="dsbD"
                     /locus_tag="QBF46_001093"
     CDS             complement(25218..26915)
                     /gene="dsbD"
                     /locus_tag="QBF46_001093"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDQ6673305.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRIVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(26891..27229)
                     /gene="cutA"
                     /locus_tag="QBF46_001094"
     CDS             complement(26891..27229)
                     /gene="cutA"
                     /locus_tag="QBF46_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDQ6673306.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(27345..28646)
                     /gene="dcuA"
                     /locus_tag="QBF46_001095"
     CDS             complement(27345..28646)
                     /gene="dcuA"
                     /locus_tag="QBF46_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDQ6673307.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(28764..30200)
                     /gene="aspA"
                     /locus_tag="QBF46_001096"
     CDS             complement(28764..30200)
                     /gene="aspA"
                     /locus_tag="QBF46_001096"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDQ6673308.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAIEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            30537..31013
                     /gene="fxsA"
                     /locus_tag="QBF46_001097"
     CDS             30537..31013
                     /gene="fxsA"
                     /locus_tag="QBF46_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDQ6673309.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(31029..32285)
                     /gene="yjeH"
                     /locus_tag="QBF46_001098"
     CDS             complement(31029..32285)
                     /gene="yjeH"
                     /locus_tag="QBF46_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDQ6673310.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            32561..32854
                     /locus_tag="QBF46_001099"
     CDS             32561..32854
                     /locus_tag="QBF46_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDQ6673311.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            32898..34544
                     /gene="groL"
                     /locus_tag="QBF46_001100"
     CDS             32898..34544
                     /gene="groL"
                     /locus_tag="QBF46_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDQ6673312.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            34682..35035
                     /locus_tag="QBF46_001101"
     CDS             34682..35035
                     /locus_tag="QBF46_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDQ6673313.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(35228..36097)
                     /locus_tag="QBF46_001102"
     CDS             complement(35228..36097)
                     /locus_tag="QBF46_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDQ6673314.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(36492..37520)
                     /gene="epmB"
                     /locus_tag="QBF46_001103"
     CDS             complement(36492..37520)
                     /gene="epmB"
                     /locus_tag="QBF46_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDQ6673315.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLSAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            37562..38128
                     /gene="efp"
                     /locus_tag="QBF46_001104"
     CDS             37562..38128
                     /gene="efp"
                     /locus_tag="QBF46_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDQ6673316.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            38180..38305
                     /gene="ecnA"
                     /locus_tag="QBF46_001105"
     CDS             38180..38305
                     /gene="ecnA"
                     /locus_tag="QBF46_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDQ6673317.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            38416..38562
                     /gene="ecnB"
                     /locus_tag="QBF46_001106"
     CDS             38416..38562
                     /gene="ecnB"
                     /locus_tag="QBF46_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDQ6673318.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            38738..39055
                     /gene="sugE"
                     /locus_tag="QBF46_001107"
     CDS             38738..39055
                     /gene="sugE"
                     /locus_tag="QBF46_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDQ6673319.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(39052..39585)
                     /gene="blc"
                     /locus_tag="QBF46_001108"
     CDS             complement(39052..39585)
                     /gene="blc"
                     /locus_tag="QBF46_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDQ6673320.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGASTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPSS"
     gene            complement(39674..40807)
                     /locus_tag="QBF46_001109"
     CDS             complement(39674..40807)
                     /locus_tag="QBF46_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDQ6673321.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMACWVRSNMNHRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(40870..41229)
                     /gene="frdD"
                     /locus_tag="QBF46_001110"
     CDS             complement(40870..41229)
                     /gene="frdD"
                     /locus_tag="QBF46_001110"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDQ6673322.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(41240..41635)
                     /gene="frdC"
                     /locus_tag="QBF46_001111"
     CDS             complement(41240..41635)
                     /gene="frdC"
                     /locus_tag="QBF46_001111"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDQ6673323.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(41646..42380)
                     /gene="frdB"
                     /locus_tag="QBF46_001112"
     CDS             complement(41646..42380)
                     /gene="frdB"
                     /locus_tag="QBF46_001112"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDQ6673324.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(42373..44181)
                     /gene="frdA"
                     /locus_tag="QBF46_001113"
     CDS             complement(42373..44181)
                     /gene="frdA"
                     /locus_tag="QBF46_001113"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDQ6673325.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            44506..45483
                     /gene="epmA"
                     /locus_tag="QBF46_001114"
     CDS             44506..45483
                     /gene="epmA"
                     /locus_tag="QBF46_001114"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDQ6673326.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            45702..47204
                     /gene="yjeM"
                     /locus_tag="QBF46_001115"
     CDS             45702..47204
                     /gene="yjeM"
                     /locus_tag="QBF46_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDQ6673327.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(47355..50678)
                     /gene="mscM"
                     /locus_tag="QBF46_001116"
     CDS             complement(47355..50678)
                     /gene="mscM"
                     /locus_tag="QBF46_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDQ6673328.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(50700..51668)
                     /gene="psd"
                     /locus_tag="QBF46_001117"
     CDS             complement(50700..51668)
                     /gene="psd"
                     /locus_tag="QBF46_001117"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDQ6673329.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(51765..52817)
                     /gene="rsgA"
                     /locus_tag="QBF46_001118"
     CDS             complement(51765..52817)
                     /gene="rsgA"
                     /locus_tag="QBF46_001118"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDQ6673330.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            52912..53457
                     /gene="orn"
                     /locus_tag="QBF46_001119"
     CDS             52912..53457
                     /gene="orn"
                     /locus_tag="QBF46_001119"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDQ6673331.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            53668..53743
                     /locus_tag="QBF46_001120"
     tRNA            53668..53743
                     /locus_tag="QBF46_001120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53701..53703,aa:Gly,seq:gcc)
BASE COUNT        13367 a        13211 c        13681 g        13567 t
ORIGIN      
        1 gccgcgcaaa aagtatgaca tttttgtttg cagcaatttt tatgaaaacg ttgccatact
       61 caaaaggcct aaggccaggc ggcgtaatgt tatttaaaca attacgcctt cagcggaata
      121 gtggttacgc tttcacacac ataacgtggt aaataccgtc aataatttca gtcccttcag
      181 tttcgtgttc aaatccaggg aaatggtggt cccaggattg cagcgagcgt aaataactta
      241 cttgcggact gtttttatcg ccgaagtttt caccagacag cagcatcggg attcctggcg
      301 gatacgggat aactgagttt gccgcgatgc gtcctggcag attttcaatg gataccagtt
      361 cgacattgtt gtcgacaatc gcgttgtacg cttcacgcgg ggtgatttcc gccaccggca
      421 ggccggaata ggcttcgttc aaccgtgcgc cagggttgtt ttctttcagc caggcaaaca
      481 tggtgtcacc cagatcgtga atccccatgt tcgcgtaagt gtcaggatat tgttcaacaa
      541 gttccggcat cacctgcgcc agcggtgtgt tggcgtcata gtggcgtttg aaggagcaaa
      601 gggtgttaac cagagtcccc catttcccac gggttacgcc catagagaac aggaacataa
      661 tttggaagtc agtggtgcgg gtaggcacaa tgccatggcg accaagccag gcagtgacca
      721 gcgccgccgg aacaccggtt tcttccagtt caccatcttc acccattccc ggagcaagga
      781 tgctgacttt aatcgggtcg agcatactcc agttatccgg aatatctttg aagccgtgcc
      841 agctttcgcc cggatgcatt acccagcagt cctgaacggt ggtcagcagt tttgttggtg
      901 cgtcagcaaa gtcataggtt ttgccggttt gtgggtcggt gacgacttct ttgttccacg
      961 gtttgaagaa ccagctaccg tcagcggtga actctttata cagccgcgcc atcgcctgac
     1021 gaaaatcaac cgcttcgtca atcacttctt gtgtcagtga caggccgctg ttgccgtcca
     1081 tcatcgacac cgccacgtcg ttggatgcgc agatggcata cagcggggag gtggtggcat
     1141 gcatcatgta ggcctggttg aagcgggaga agttaatcgc cccacgacct tcacgtacat
     1201 gaatataaga agcctgtgac agcgcattca gcagtttgtg ggtggagtgg gtggcgaaaa
     1261 cggtaggacc gttgtgatcg ccaggttcgc cgcgcatggc atagtgatcg gcatagatcg
     1321 ggttgaaacg tgcatagccg taccaggctt cgtcaaagtg cagacgatcg gaggtttttt
     1381 ccagcagatc ctgcgcttct ttagcgttat aacacacgcc gtcataggtg cagttggtca
     1441 ccacgcagta agacggtttt tgcccagctt tgtctttggt cagcgggctt tcactgattt
     1501 tcttctgcaa ggtttcaggt tgcatttcct gcggatagat tggcccgata atgccgtagc
     1561 ggttgcggct tggcaccata tagaccggtt tcgcgcctgt cagcatcaaa ccttgttcga
     1621 tggatttatg gcagttacgg tcaacgacca cgacatcgtt atcggtcatg caagcctgca
     1681 tgatggtgcg gttagagccg gaagtaccga cgactaccga ccaggagcga tcggcaccaa
     1741 atacgcgtgc ggcatatttt tcgctttcgc caaatgcgcc agtatggtca agcaaagaac
     1801 cgagggaagt tcgttcgatg cccatgtcgg tgcggaacag attttcacca tagtagtcat
     1861 ggtagaaacg tccggcgggt gttttggtaa aaccaacgcc gccctggtgg cctggcgctg
     1921 cccaggaata ttcatggatg tcactatatt tcatcagcgc gctgaacagt ggcggcaaca
     1981 gctgctggcg gtagcgggtc atcgcggcaa cggcgcgtcc ggcgataaag tcggcggtat
     2041 cttccagaat ccaggcgaat tcatcgacaa gctccagcag gtcgcgatcc attgcggcga
     2101 gggctttttc ccgatcgccc aacaggaaga ccggcacgtt ttgttggcgc tcatgaagct
     2161 taccgatcaa ttgtctgacg ttttgatgtt cgtccggatg ttccatttga tagctgaaca
     2221 tcaggcagtc aatggcttcg tttgaagaga gaatggcaaa accatcatca aaggaggtgg
     2281 atttaatcac ggtaacattt tgctggctta aagcatctgc cagacgctca acggcgttac
     2341 cgacccaggt gtcttgatgg agaaactcgc tttcaacaat taatactttc atcattgctt
     2401 acccggttat gaaggaatct tcgttgcggg caagtatctt ccggcctttt cgggctgtaa
     2461 agcgcgtgaa aaacaaacat ttttgaaata tttattttta taaacaatgc gttatgtgat
     2521 tattttaaat tattttcact gcttattatc gacggctaaa ctattttttt ggctgatact
     2581 gatatcgtct ttagccggga aacgtctggc ggcgctgttg gctaagtttg cggtattgtt
     2641 gcggcgacat gccgacatat ttgccgaacg tgctgtaaaa acgactactt gaacgaaagc
     2701 ctgccgtcag ggcaatatcg agaatacttt tatcggtatc gctcagtaac gcgcgaacgt
     2761 ggttgatgcg catcgcggta atgtactgtt tcatcgtcaa ttgcatgacc cgctggaata
     2821 tccccattgc atagttggcg ttaagtttga cgtgctcagc cacatcgttg atggtcagcg
     2881 cctgatcata gttttcggca ataaagccca gcatctggct aacataaaat tgcgcatggc
     2941 gcgagacgct gtttttgtgt gtgcgcgagg ttttattgac cagaatcggt tcccagccag
     3001 agaggctaaa tcgcttgagc atcaggccaa tttcatcaat ggcgagctgg cgaatttgct
     3061 cgttcggact gtttaattcc tgctgccagc ggcgcacttc aaacgggcta agttgctgtg
     3121 tggccagtga tttgatcacc atgccgtgag tgacgtggtt aatcaggtct ttatccagcg
     3181 gccaggagag aaacagatgc atcggcagat taaaaatcgc catgctctga caggttccgg
     3241 tatctgttag ttggtgcggt gtacaggccc agaacagcgt gatatgaccc tgattgatat
     3301 tcactttttc attgttgatc aggtattcca catcgccatc gaaaggcaca ttcacttcga
     3361 cctgaccatg ccagtggctg gtgggcatga tatgcggtgc gcgaaactca atctccatcc
     3421 gctggtattc cgaatacagc gacagcgggc tgcgggtctg tttttcgtcg ctgctgcaca
     3481 taaacgtatc tgtattcatg gatggctctc tttcctggaa tatcagaatt atggcaggag
     3541 tgagggagga tgactgcgag tgggagcacg gttttcaccc tcttcccaga gaggcgaggg
     3601 gagcgtccga gcatcatgag gccgaaaact gtgcttttca ggtaacttat taccataaac
     3661 tcagatttac tgctgcttca cgcaggatct gagtttatgg gaatgctcaa cctggaagcc
     3721 ggaggttttc tgcagattcg cctgccatga tgaagttatt caagcaagcc aggagatctg
     3781 catgatgact gcacccaaaa ttacatttat cggcgctggt tcgacgattt tcgttaaaaa
     3841 tattcttggt gatgtgttcc atcgcgaggc gctgaaaacg gcgcatattg ccctgatgga
     3901 cattgatccc acccgcctgg aagagtcgca tattgtggtg cgtaagctga tggattcagc
     3961 aggggccagc ggcaaaatca cctgccacac ccaacagaaa gaagccttag aggatgccga
     4021 ttttgtcgtg gtggcatttc agattggcgg ttatgaacct tgcacggtga ctgatttcga
     4081 ggtctgtaag cggcatggtc tggaacaaac cattgccgat acgttggggc cgggcggtat
     4141 tatgcgcgcg ctacgtacca ttccgcatct gtggcaaatt tgcgaggaca tgacggaagt
     4201 ctgccccgat gccaccatgc tcaactatgt taacccaatg gcgatgaata cctgggcgat
     4261 gtatgcccgc tatccgcata tcaaacaggt cgggctgtgc cattcggtgc agggaacggc
     4321 ggaagaactg gcgcgtgacc tcaatatcga cccggctacg ctgcgttacc gttgcgcagg
     4381 tatcaaccat atggcgtttt acctggagct ggagcgcaaa accgccgacg gcagttacgt
     4441 gaatctctac ccggaactgc tggcggctta tgaagcaggg caggcaccga agccaaatat
     4501 tcatggtaat actcgctgcc agaatattgt gcgctacgaa atgttcaaaa agctgggcta
     4561 cttcgtcacg gaatcgtcag aacattttgc cgaatatacg ccgtggttta ttaagccagg
     4621 tcgtgaggat ttgattgagc gttataaagt accgctggat gagtacccga aacgctgcgt
     4681 cgagcagctg gcgaactggc ataaagagct ggaggagtat aaaaacgcct cccggattga
     4741 tattaaaccg tcacgggaat atgccagcac aatcatgaac gctatctgga ctggcgagcc
     4801 gagtgtgatt tacggcaacg tccgtaacga tggtttgatt gataacctgc cacaaggatg
     4861 ttgcgtggaa gtagcctgtc tggttgatgc taatggcatt cagccgacca aagtcggtac
     4921 gctaccttcg catctggccg ccctgatgca aaccaacatc aacgtacaga cgctgctgac
     4981 cgaagccatt cttacggaaa atcgcgaccg tgtttaccac gccgcgatga tggacccgca
     5041 tactgccgcc gtgctgggca ttgatgaaat atatgctctt gttgacgacc tgattgccgc
     5101 ccacggcgac tggctgccag gctggttgca ccgttaaaac gcgactaaac gctactgcgc
     5161 cgggggattt attccggcgc acacctctga cgataccaat aacagaaggc gggcgttggt
     5221 aacagcgacc cgatacccta tgagcatttc aatgactaca aaactcagtt atggatttgg
     5281 agcgttcggg aaggattttg cgatcggcat tgtgtatatg tacctcatgt attactacac
     5341 cgatgtcgtt gggctgtctg tgggtttggt cggtactttg tttctggtgg cgaggatctg
     5401 ggatgctatt aacgatccga ttatgggatg gattgtaaat gctacgcgat cgcgatgggg
     5461 taagttcaaa ccctggatcc tgatcggtac gttggcaaac tctgtaatct tatttctcct
     5521 ctttagtgcg catctgtttg aaggtactac tcagattgtc tttgtttgcg tgacctacat
     5581 cctctggggc atgacttaca ccattatgga tattcccttc tggtcgctgg ttccaaccat
     5641 aacgctcgat aaacgtgagc gcgaacaact ggttccttat ccgcgttttt ttgccagtct
     5701 ggcaggcttt gttacggcag gtgtgacgct accatttgtt aattatgtcg gcggtggcga
     5761 tcggggattt ggctttcaga tgttcactct ggtactgatc gcctttttta ttgtttcaac
     5821 catcatcact ctgcgcaatg tgcatgaagt cttttcgtca gacaatcaac cgtctgctga
     5881 aggaagccat ctgacactta aagccatcgt tgcgctaatt tataaaaacg atcagctttc
     5941 atgcctcttg ggtatggctc ttgcttataa tgtagccagc aacattatta ccggctttgc
     6001 tatctattat ttctcatatg ttatcggtga tgcggatttg ttcccctatt atctgtcgta
     6061 tgcgggagct gctaacctgg tgacgttagt attcttccca cgcttagtta aatcattatc
     6121 ccgacgcatt ttatgggccg gagcatctat tcttccggtg ttaagctgtg gtgttctcct
     6181 gttaatggca ttaatgagct atcacaacgt cgtcctcatt gtgattgcgg gtattttgct
     6241 gaatgtggga tcggcgcttt tctgggtatt acaggtcatc atggtggcag ataccgttga
     6301 ttacggtgaa tataaactgc acgtacgctg tgaaagtatc gcttactccg tgcagactat
     6361 ggtggtgaag ggcggttcag cctttgcggc ttttttcatt gcggttgtgt tagggatgat
     6421 tggctatgta ccgaatgttg aacagtctac gcaagcccta ttaggtatgc agtttattat
     6481 gattgctcta ccaactctgt ttttcatggt aacgctgatt ctctacttcc gtttctatcg
     6541 cctcaatggt gacacgctgc gcaggatcca gatccatctg ctggataaat atcgcaaagt
     6601 accgcccgag cctgttcatg ctgatattcc ggtcggtgca gtgagtgatg tgaaagcctg
     6661 acgtggacaa tagctaacga aaatggcctg atgcgatatg tttatcaggc cacgtcttaa
     6721 tgtaatgctt tggatttgca aattttgtag accggataag gaattcacgc cgcatccggc
     6781 atcaacaaag cgcaagttgt tatccggtta tcaagccaaa gcgccgtagc tggcggcaat
     6841 gaaagcgagc gaggaatatc accgtcgtta atgcgccaag cagcgcgcag aacatatcgg
     6901 attgcgtgtc ccactggtcg ccctgggtgc cgagaaaatc atccgctccc tgacccattg
     6961 ccagcgctgc ccaccactcg attaattcat acatcgcgct tatcgccagc gccacgcagc
     7021 agaccaggaa cgccaccatt ttacgtccgc gcacgtacat cccgcgaacg agaatttctc
     7081 gtgccaccag tgcaggcacc agcccctgga aaaagtgccc cagcttgtca tacggattac
     7141 gactcaaccc cagccattcc tgcacctcaa aaccaacggg gactttcgcg taggtgtatt
     7201 gtccgccgac catcaggatg atggcgtgaa ggaaaatgag cgtatagagc agcggcgtta
     7261 acggataacg tctggcggtg gcaagcagta gcggcacgac aataatcacc ggtgtcactt
     7321 ccatcagcca ggtgagtttg tcatgggccg aaatgccggt ataaatcagg attaatgtta
     7381 gcgccagtgc gccggtgtta agaattaacg gcttgagtgt gcgggtcatg gtgagttcgc
     7441 aaatcaggga aaatactgac tattcaccag caagcgtgaa attacaatcg caaaagaggt
     7501 ggcccagggg gatcacctgg cagcatgctg ccaggcgctg ggccgaagag gttacttagt
     7561 gcagttcgcg cactgtttgt tgacgatttg ctggaagaag tcgttacctt tgtcatcgac
     7621 caggataaac gccgggaaat cttctacttc gattttccag atagcttcca tacccagctc
     7681 cggataagcg acgcactcca gatgcttgat gctctgctgc gccagtaccg ccgccggacc
     7741 gccgatgcta ccgaggtaga agccgccgtg tttatgacac gcgtcggtaa cctgctggct
     7801 gcggttgcct ttcgctagca tgatcatgct gccgccgtgg gattgcagca gatccacgta
     7861 ggagtccata cgacctgcgg tggttgggcc aagtgaacct gatggataac cggcaggggt
     7921 tttcgccgga cccgcgtagt agatcgggtg atctttgatg tactgcggaa gttctttacc
     7981 ggcgtcaatc agctctttca gcttggcgtg tgcaatatct cggcccacga taatggtgcc
     8041 ggtgagcgac aaacgagtgg ataccgggta ttgcgaaagc tgggcgagga tctctttcat
     8101 cgggcggtta aggtcaactt tcaccgcttc gccttcaccg gcctggcgca gttcttgtgg
     8161 aatgtactgg cctgggttgt gttccagttt ttcgatccag ataccttcgc ggttgatttt
     8221 cgctttaatg ttacggtcag cggagcagga gacgcccatg ccgaccgggc aggatgcgcc
     8281 gtgacgtggc agacggataa cgcgaatgtc gtgcgcgaag tatttaccgc caaactgcgc
     8341 gccaagaccg agtttctggg cctcttccag cagttcctgt tccagctgga catcgcggaa
     8401 cgcctgacca tgttcgttcc cttccgtcgg cagttcatcg taatagtgag cgcttgctaa
     8461 cttgacggtt ttcaggttgg tttccgcaga cgtaccgcca atcacaaacg cgatatggta
     8521 cggcgggcag gctgcagtac cgagggtacg cattttctcg acgaggaagt ttttcagttt
     8581 gccgggagtc agcagggctt tggtttcctg gtagagatac gttttgttgg cagagccgcc
     8641 gcctttcgca acgcaaagga atttgtactc atcgccatct accgcgtaca ggtcgatttg
     8701 cgcaggcagg ttagtgccgg tgttgacctc tttgtacatg tccagcgccg cgttttgcga
     8761 gtagcgcagg ttatcttcga tataggtgtt atagacgcct ttcgacagcg cttcttcatc
     8821 accgccaccg gtccacacgc gctggccttt tttaccgacg atgatcgcgg tgccggtatc
     8881 ctggcaggtc ggcagcacgc ctttggcggc gatttcggag tttcttaaga attgcagtgc
     8941 cacgtacttg tcgttttcgc tggcttctgg atcgtgaaga atagccgcaa cctgtttctg
     9001 gtgtgccgga cggagcatga aagaagcgtc gtgaaaggct tgctgcgcca gcagggtcag
     9061 ggcttctggt tccactttca ggatggtttc gccgtcgaag tcggcaacgc taacgtaatc
     9121 ggaagtgagt agatagtatt cggtattgtc tttccccatc gggaaaggtg cctggtagat
     9181 aaagggtttg tttgacatag cttccagcct gtaactctgt atttgttatt cgaaaatggc
     9241 gtgccgcgta gtcacgggcg gcacgcaaag tgcatttata agaacccgta catcgcggcg
     9301 aagatccagc cgaagacgca cgatacgctc acaccaatca accccggcag aataaagctg
     9361 tggttgatga cgaagcgacc gatgtgggtg gtgccggaac ggtcaaactg aatcgctgcc
     9421 agatcgctcg gataagtcgg caggatgtaa taaccgtagc aagccggtgc tgaagccacg
     9481 atgtatgccg gatcaacgcc gatcgccagc gcgaccggaa caatcgccgc cagcgccgca
     9541 gcctgagagt ttacaaactt ggaaaccagc agcagaacaa tggcataggc ccacggatac
     9601 tctttcacca tttcacccag tacgccctga atttcagaca tatgcgcacc gaacatggtt
     9661 tctgccatcc atgcgatacc gtacaccgcc acgatggcga tcataccgga acggaagact
     9721 tcgttttttg agatagacgc gggattggtt ttggtcagga taataatcag cgccccggtc
     9781 agcagcataa acatctgaat aaccagtacc atcgacagcg gtttgccgcc gaaggatgga
     9841 cgcaggtccg aatcagcacc aagaagggcg actacagcga ttgccccgag gaaaatccac
     9901 attgccagcc agttgctttt cggcagtttt ttatccagca gcgtcgcggt atcaccgtaa
     9961 acatactcac ggttttccgg tacggagatg aatttctgga actcttcgtc tttatccaga
    10021 tctttaccgc ggaaccagct gaagataccg atcgccagga taccgattaa cgtcgatgga
    10081 atggtgattg ccagcagatc gaggaactca agatggcggc catcaaaggt gacattaccc
    10141 agcatcgcaa ccagagacac gaccgcaacc gataccggac tggcgataat ccccatctgt
    10201 gcaccgatag aacttgccgc catcggacgt tccggacgga tgttgttctt aatggcgacg
    10261 tcgtagatga tcggcagaat ggtgtaaacc acatgacccg taccgcaaag aatggtcagt
    10321 gtacaggtca caaacggcgc gacaattgag acatatttcg ggttgcggcg cagcagcttc
    10381 tcggcaattt gcagcatgac atcaagaccg cccgaagctt gcaaggtcgc cgatgccgcc
    10441 accaccgcaa tgataaccag catgacatca actggtggtt tacctggctg aaggtggaag
    10501 acgaagacca gaatgaccag accgataccg cctaataaac ccagcgcgat accacccttt
    10561 ctggcaccat aaaacagaca tatcagtatt atgataagtt ggatagtaaa taacatgtgt
    10621 gaaccctcgc gataatccta tttaaatttt tgctgaatag atcacagtca cgttctgttt
    10681 tgtatgaact gttttcagag cctgactaaa tatccgtttg gtcgttaccg gctttagcaa
    10741 atacctcaca gtgaatattg gctgattaaa gcagcggcaa atttatgcac tgtgataagc
    10801 ggctttttca gagagaaaga ctctctggcg atgggtttat agtttgtact gcgcagatgt
    10861 taatgttttg tttgctgtta tcttattgat atttatcggt ctattttttg ttctcagggc
    10921 agatgtcatt aggtctatag attaatctga tctacccatt tgtgggtaaa aatacacata
    10981 atgcgggtga cataatagtt aattaacttt tgttagcgtt ttgaaattaa aaacaccgtt
    11041 cacctgaaga gatattaatt tttagcgatg atggagggat aattatattt gatctggcac
    11101 aagttttact gatgaaggat gtaacttgtg ccaggggtat tttgcattac ggtaataatt
    11161 attacttaca gataaccagc gatccgttat tggcaatatt gtttcagtag tgagtagtgt
    11221 tctgcctgaa tacggtaacg gtaaactgga cgccccgtga cgccataatg gatactggtg
    11281 aacaagatgt ggcagttgac cagccagatg aggtatttac ggcaggaaac acgcgagatg
    11341 ttaacctcgt tagccagctc gtcggttgaa aactcatagt cctgatgcgc gtcaatccac
    11401 tggcacagcg tgcgtaacgt ctgcggcgtt aagccttttg gcaagcgacg aggatcctgt
    11461 tcgttggagc tgctgccgtg gattagctga tcaagctcgg cctggtcata atactgatgt
    11521 ttttccagcg ccattttctt ttgccgccag ccggtgagcg cctcttcaaa gcgggaagcc
    11581 tggaagggtt tgatcaggta atcaacgaca ccgtaatgca gcgaatcttt aatggttgcc
    11641 gcatcggctg cggaggagat gacaatcaca tcacttttgc aacgcgcgtt atgcaggaca
    11701 ggcagtaaat cgagcccgtt ctctttttgc atatagatat cgagcaatat caggtcgata
    11761 ggcgtatcgc tattgaagat aatctctttg gctttctcca gcgtcgaggc tgttccacag
    11821 cattgaaagc ctgggatttg tgctacgtat cggcgattca gctccgcgac cattgcgtcg
    11881 tcatcgataa ttaatacatt gatcatctgt tcgacctctc cccgtcccag ggtatctgga
    11941 caaaaaattg tgtgaaaatc ccgggttccg attccacggc gatgctgccg ccgagatttt
    12001 ctacctgttg tttgacaagt gctaaaccga cgcctcgctc gcttcctttt gtcgagacac
    12061 ctttgtcaaa aatgtgatcg attttgtcgg gtgcgatccc cggtccatca tcgttaactt
    12121 cgcagtgcag ccagccgtga cggtagtgca atgttacgct aatttcgcca ccgggttccg
    12181 gccctaatgc ttccagcgcg ttttctatca gatttcccaa tgtggtaatc agcgtcgcga
    12241 cctggtcctc actgccgctg tctggcagca ggctttcact gtttaaaatc agcgtatggc
    12301 ctaaatcggt cgcgcggtta atcttgctga ttaaaaaacc agcgataacc ggagatttga
    12361 tcttacccag cagagagcca atctcttcct gatagttatt ggctgttttg agaatgtaat
    12421 cttccaactg cttataactc ttcagatgca ataatccgag aatcacatgc aatttattca
    12481 taaattcgtg ggatcgttca cgaagtgcat cagcatagtt gaccagaccg tcgagtcgct
    12541 gcatcagttt acgaacttca gttttgtccc tgaaggttga aatggcaccg atgataacgc
    12601 cattactgcg caccggaacg gtgttgatca gtaatagccg gtctttaatc gtaatctctt
    12661 cgtcgcggcg cggcgtaccg tcgcgtaaca cttccgagac atctaccacc tgtgaccatg
    12721 agtggcttag cgtcgacagt ttctcatcgt cctgcgactt acggtaattc agcaattctt
    12781 gtgcggcatc gttgatcagc gtgacctcgc cgcgatcgtc cacggcaacg acgccttctt
    12841 tgatagactg caacatggcc tggcgctgct caaacagcgt ggagatttcg tagggttcca
    12901 ggccgaaaag gatttttttc agtaccttaa ccagaatgca ggtgccaatc agtccgacca
    12961 gcatgccaaa taataccgac cagataatgc tccagcgact gtcattgatt tgttgggtca
    13021 cgcggcttaa ctcaaggccg atcgccacca cgccaatttg tttatgattt tcatcgtaga
    13081 tgggggtaaa tacgcgtaaa gcctgcgcca gaaaaccgcg attgatagcg acattttctt
    13141 tgccattcag cgctttaagg atgtcatcac ctttaaatgg ctgaccaata cgctgggctt
    13201 caggatgcga gtagcgaaga ctttgcatat cggtaacgac aataaacagc agatcgttgc
    13261 gtttgcgcac ggcttccgcg atggcctgga ttccgctctc ctgcggtttt ttctgcaagc
    13321 cctgacggat ttccggcgag tcggcgaggg tacgcgccac tgccagtgcc ttgttggcca
    13381 gcccgtctcg cgtcatatca ctgatttgcg agaagtaaat cagatgcacc accaatagca
    13441 ccgagaacag taccgcgctg accattaaga tcactgtggt actcaatttc atcggacgtt
    13501 tgcgtaacat gcggtagggc aatgaatgtc tcatcagctt ccttgtgtga caaatttctt
    13561 aagcattatc tctgatgagg cgggtaattc aaagggagta agaatgattg gctatatagg
    13621 ggaagagact ctggcaacgg aaactgccag tgctgtatga agattccagg gctatgctta
    13681 tagcgataat catactgatg agagagggaa agtcatggat caggcgctac tggacgaggg
    13741 ttatcgctgt tataccggcg aaaagatcga tgtctatttc aacactgcga tatgtcagca
    13801 ttctggcaat tgcgtacgtg gcaacggcaa gttatttaat ctcaaacgaa agccgtggat
    13861 catgccggat gaagtcgacg tcgtcactgt ggttaaagtg attgatacgt gcccgagcgg
    13921 cgcgctgaaa taccgtcata aataagcgag ggtaaaatgg aaatacgcga aggccacaat
    13981 aaattttaca ttaatgacga acagggcaag caaatcgctg aaattgtctt tgtgccgacc
    14041 ggagagaatt tagcgattat cgaacatacc gatgtcgatg aaagcctgaa agggcagggg
    14101 attggtaaac agctggttgc gaaagttgtg gaaaaaatgc gtcgggaaaa acgaaaaatt
    14161 atcccattat gtccatttgc gaaacacgaa tttgataaaa cgcgggagta tgatgatatt
    14221 cgcagttgat gggagagtac agattcacga tatttttcat gctctctgcg gtgtgatgca
    14281 ggagagcatc tgaagggtag ggggatgcac aaagaatggg cagagagcgc gtttttttgt
    14341 cccaagtcat cccctttact gagcaaaaaa aagaatatct cctatatgag aatcatcaat
    14401 cggggttaat aagttttgcg tccccagagt gttcaatatt gataggagtc atattatgga
    14461 aggtaaaaac aagttcaata cttatgttgt ttcttttaat tatccatcat cttattcctc
    14521 agtgttctta aggttaagat cattgatgta tgatatgaat ttctcctcta tcgtggctga
    14581 tgaatatggg ataccacgac aattgaatga aaactccttc gcaataacga catcgttagc
    14641 cgcaagtgaa atcgaagatt taatcaggct taaatgctta gacttaccgg atattgactt
    14701 tgacctcaac attatgacag ttgatgacta tttccgtcag ttttacaagt agccaggaag
    14761 gataagaaga acataaaaaa tggcactatt ctctaaaata ttaatttttt atgtgattgg
    14821 tgtgaacata tcctttgtca ttatctggtt tatctcacat gagaaaacac atattcgttt
    14881 acttagtgca ttcctggtcg gaataacctg gccaatgagt ctgcctgtgg cattactttt
    14941 ttctctcttt taggagctgt agttgagtgg acccgctggt tatcgtgaaa cgaagcgtct
    15001 ctcctcaaaa ctggatttgc ggaacgatga acgaagcagt cgcctgactg cttcgttcat
    15061 taaagtgaaa tttatttctg tgggcgcatc gccgggaaga gaataacgtc acgaatagta
    15121 tgactgtttg taaacagcat aatcattcgg tcgatgccaa tacccagacc agcggttggc
    15181 ggcagaccat attccagcgc agtcacgtaa tcttcgtcat agaacatggc ttcgtcgtca
    15241 cctgcagctt tagcgttaac ctgttcctgg aaacgttcag cctgatcttc tgcgtcgttt
    15301 aattcgctaa aaccattacc gatttcacga ccaccgatga agaattcaaa acggtcggtg
    15361 atttccgggt taacatcatt acggcgcgcc agcggggaca cttctgccgg atattccgta
    15421 ataaaggttg gctgaatcag atgtgcttct gccacttcat caaagatctc cgtgacaata
    15481 cgtcccagcc cccagctttt ctctaccgta attccgatag attcagctaa tgctttagca
    15541 gcatcaaaat tatccaggtc ggccatatcg gtttctggac gatatttttt gattgcttcg
    15601 cgcatggtga gtttttcaaa cggtttgccg aaatcaaaca catgctcgcc ataagtgact
    15661 ttagtggtac ccagaacctc ttgtgccagg gtgcggaaca gtgactctgt cagttcaatc
    15721 aaatcgtggt aatccgcata cgccatgtag agttccatca ttgtgaactc aggattatgg
    15781 cgaacagaaa taccttcatt acggaagtta cggttgattt cgaatacccg ttcaaaaccg
    15841 cctacaacca gacgtttcag atacagctcc ggcgcgatac gcagatacat atctaaatcc
    15901 agagcattat gatgggtaat aaacgggcga gcagatgccc cacctggaat tacctgcatc
    15961 atcggggttt ctacttccat aaagccgcgc gcgaccatga actgacggat agcggccaga
    16021 atttttgaac ggacaacaaa cgtttgacgg gatttatcgt tagcgatgag gtccagataa
    16081 cgttgacgat aacggacttc ctgatcctgc agaccatgga atttatctgg taaaggacgt
    16141 agtgcttttg tcagcaggcg caactcagta cagtgaatgg aaagctcacc cgtttgcgtc
    16201 ttaaacagcg taccgcgggc accgataatg tcacccagat cccatttttt aaactgatcg
    16261 ttataaacac cttctggcag gctatctctt gcaacgtaca gttgaatacg gccaccgaca
    16321 tcctgcaacg ttacaaagga ggctttcccc atgatacgac gggtcatcat tcggccagca
    16381 accgagactt caatgtttaa ggattccagt tcctggttat ccttcgcatc aaactcttcg
    16441 tgcaactggt cagaggtatg gtcgcggcga aaatcattgg gaaacgccac accttgctga
    16501 cgtagtgccg ccagtttttc gcggcgattt ctcagttcat cgttaaaatc aatagcctca
    16561 ttggctcccc gtgtttcttg ttcagacatt ttggttcctc taaatccagc tttcaattta
    16621 gcgtagataa agagacagat cggtacactg tattatctgc ctcgactatt agtaactcag
    16681 tttatcttat cgaaactaac gcttacctca agttgatatc actaataaat aaccatcaaa
    16741 atggtttaaa tgacttatta atctcgattt gttagtgtta taaactgaaa gttaatttat
    16801 tcataaccaa ttgtttttac gacagttaat cgttgctctc ctgtatcata ttcgtcggcg
    16861 tgctaaacag aaaacgggta gcaaaggcca atacgacgat gatcgcgaca caagccaacg
    16921 tccactctcc catctgagaa aagaatcgct ggtaagctgc aattgccata ccgctaattt
    16981 gcgactctgt cgtctgctgt gccacgacgc ctgccagcca gttggcgacc gcgcctgttg
    17041 ccagcatata aatcccggtt aatacgccag acattttcag acgcgtgatt tgcgcaatcg
    17101 ccaccgggtc aataaagagt tcggcaaagc ccattagcgc cagcccggat atcatcacgc
    17161 ccattgacgc ttgaccgtca gccgctgcat gtcgggcatc aaatgccaac aacataaagc
    17221 cacaagccat cagcagtaag ccaaaggcaa acttcagcca gacgcgcaat gttgagttgc
    17281 cgcggctttc tggcgacgcc agccaggcca gtacaacccc agcgagcatc accgcaattg
    17341 cattcaccga ctggaatagt gctgtaggta cttcaatatt gaatgcctga cgattcacaa
    17401 agcgatcgat aaacaagctg atggtactgc cgccctgttg tgccagtacc cagaacaatg
    17461 tcccgacaaa catcaacaat acaatttgcc aaagagcacg gcgatgttcg gggaatttga
    17521 tcatcatgcg ggcaatgatt tgtgcggcaa tgaggcaaac gatcgccagc aaatatccgg
    17581 accagtcgtt ctccagcagc agggtaaaaa atactggggc taaacagagc atcaccacta
    17641 accagctcca tactggtaag gcaaatttga cgctagtgag cgctttttta tccatactac
    17701 gcgtggactg gaaatgacga tgcccgctta agaaaatcaa caaaccgata aacatgccgc
    17761 cacccgcaag ggcaaagcca acatgccatc cataccactg agcagccagg ccgcaggcga
    17821 ttggggcggc gatagaaccg atattgcccg cagcatacag cagcgaaaaa ccgccatcac
    17881 gtcgatgatc gttctcgtcg tagagctcgc caagcaaaca gctgatgttt gatttgaata
    17941 aaccgtagcc acaaataatg attgccagcg ccagatacag gctaaaggtt gaatttgtat
    18001 caatgcccag caccacatgg ccaagggtca ttaacagcgc gccggcaatc actgcagtgc
    18061 ggttgccgag caggcggtcg gcaagccagc cgccgagaat aggggtaacg taaaccagag
    18121 aagcatatgc gctgaacagg ctgatggcat ggttatcatc aaaaccaagc tgatgggtga
    18181 ggtagagaat gagtaaggca cgcatgccgt aaaaactgaa gtactcccag atttggatcg
    18241 ccacgatata gtatatcgcg cgcggctgtg agggtgtttt catgtgttct ccttatgagc
    18301 aaaaaaggga agtggcaagc cacttccctt gtacgagcta attatttttt gctttcttct
    18361 ttcaatacct taacggtata gcggccatca gcctgacggt atgcaccgtg aatatcggtt
    18421 tcaaagcccg gatagtgagc gccgatttca cacagcatct gcaggaactc cagaaccgga
    18481 cggctttctt cggtgatcat ttcacccggc attaccagag gaactcccgg cggatatgga
    18541 aggatcatat tggcgttaat acgacctacc atttcgtcga ggtaaacttc ttcggtcata
    18601 ccgtgcagct ctttctggaa ggcagcatac ggagtcatta ccatcgtcgg cagcacttca
    18661 aatgcgcgat acatcagatc cggcagattg tggtgaacaa tcagtttgtg gatattctga
    18721 gccagttcct gaatacgcat gttttcatag aattcaggat cttcacgata cagagacggc
    18781 agcatgtttt tcacacgcag gttcaggtcg aacgcacgtt tgaagtcagt cagagcacgc
    18841 agcaggctca gtgctttggt cttatcgata ccgatgctga acaggaacag caggttatac
    18901 ggaccggttt tctcaacaac gatgccatgt tcgtcgaggt atttcgccac gatgctggcc
    18961 ggaataccaa agtcgctcat ggtgccgtct ttttccatcc ccggagtcag cagggtgact
    19021 ttgatcgggt caagatacat gtgctcgtta tcgatgtttt tgaagccgtg ccaggtgctg
    19081 tcagaacgca gcggccagca ttcagtggta tcgatatgat ccggctgcca tacatcaaag
    19141 aaccagccat cagattccgt tctcagacgt ttgatctctt tacggaattt gatcgcacgt
    19201 tcaatggaac cgttgatcag acgcttacct gcattgcctt tcatcatcgc cgcagcggtt
    19261 tcagttgacg ccacgatacc gtagtgcgga gaggtggtgg tgtgcatcat gtaggcttcg
    19321 ttaaaggttt cttcgtttac gtcaccttta acgtggatca tggaagcctg agagaacgcc
    19381 gccagcagtt tgtgagtgga ctgggtttcg taaatcactt tcccttctac acggccaccg
    19441 ctcataccgc atttaccttc gtaaatcggt gagaagttgg tgtaaggcac ccacgcggag
    19501 tcaaaatgga tggatttcac atccagtgtt ttcttgatga agtcggtgtt gtacagcaga
    19561 ccatcatagg tagagttagt aattacagca tgtaccggcc aggttgcgtt tggtgtttcc
    19621 ttcacgcgct tagcaatggt agcgtgctgg aattcactct gtgggatacc accaagaata
    19681 ccgtaagcgt tacgggtcgg gcggaaatag attggcgtaa catcgctcat catcatcagg
    19741 tgggtcagcg atttgtggca gttacggtca atcagaatgg tgctgcctgc tggagcagag
    19801 tacataccaa caattttgtt cgcagtggaa gtaccgttgg tcaccatgta gctgcggtct
    19861 gcgttaaaga cgcgagcgat atactgttct gcttctttgt gtggaccact gtgatccagc
    19921 agagaaccca gttcagatac tgaaatggaa atgtcagatt tcatggtatt cggaccaaag
    19981 aaatcataga acaggctacc taccgggctt ttctggaatg cagtaccgcc catgtgacca
    20041 ggagtacaga aagtatattt accttcacga acatatttaa acagtgcttt agtcagcgga
    20101 ggcagaatag tgttgatata ttcgtcagtg gtctgcttga tcttgttagc aatatcttca
    20161 gcagcaccca gcgcatattc aaagaagcta atctgtaaac gcaggtcatt caggcttaca
    20221 tcgagagtgg aatacgtatt agcgaacgcg tacaacggca ggttctcgtt cattttgcta
    20281 atttcttcac acagctcgag attatattta tcccagtcaa aaataacgcc gcacagacgc
    20341 gcattgtttt cgatcagttt taataagtcg tcacggtcgt tcgggtaaac aatctggaag
    20401 ttcagacgtt caagcgcgcg atgaagttca cggatgggtt cttctttaaa ataaaccccc
    20461 atgtgattca atattgcaat aacgttcata gtcatatctc caggtaaaaa aggcccctcc
    20521 caacacatgg gacaaaatga aaggaggagc ctcggaaaat acttttaatt aatgtgcgtt
    20581 agacgcggtg tggttatcca ttgagtggct ctggcgctcg tgcattttgc gagcgtagaa
    20641 catcaggata atcaggctga cgatgaaggt acctgccagc tcgaaggagc ttgcgcccat
    20701 cagcgcgatg aagcagaaca cgcaacccag tacagagcag atcaggctga caaagttgcg
    20761 gatgttaacg ccttcaaaac gaatcaggtc aacgcaagag tagaagtacg gcagcatagt
    20821 cagcagtact gcgataccgg tcagttcacc gaacaggtca gatgctttac caccagcaga
    20881 gttcatcaga gtgatgagga tcatcagggc agtcattttc actgcagcca gcagcagacc
    20941 ttttttcgga ataccgttgc tgtcgacttc accataaact ttcgggaagt taccgtcgtt
    21001 agcggcacgt acacctgcct ggcctaccaa catcatccag gagcccagag aagtcaggca
    21061 cgcaaaggcg gtgaatgcag aaaccagcgg cgcagcccag ttaccgagga tagttgaagc
    21121 actgattgca aacggagcac cggaagccgc cattacagaa gacggataca taccggaaag
    21181 cacctgagtc gcagcgatgt aaacaatacc tgctaaacca gtacccagca tggttgccag
    21241 cggaacggta cgtttcgggt ttttaaccat accagtactt acagctgcgg attcaacacc
    21301 cacgaaggcc cacaggcaga gcagaatact tttaatgatc gcatgaccat cagtggtatc
    21361 cgcagtattc cagttagctg cataagttgc cgcatcaaac caatgccagc caacaatagc
    21421 agtcatcacc acaggaataa gaaccagcac cagaccaata gtggttaaac ggcttaccca
    21481 ggtaccgccg agcatattta caaaggtaaa tacccagacg atagcaatac aggcgatacc
    21541 cgccggaaca ggatcattta atactgggaa gaaggtggaa agataagata cagcggtaat
    21601 accaatcgcc aggttaccaa tccagttagc atggtaataa agaacacctg tctgaaaacc
    21661 aaatgcagga gaaatttctc cggcataagc aattgggcca ccttgttgcg ggttttttgt
    21721 tgccagtcgg gcatatacat acgccagcga cattgcacca ataatagaga taatccaacc
    21781 ccagatagca ataccaccga tacttgctag gttcgcaggt aataatgcaa taccgctccc
    21841 catcatatta ccggcaacaa caccggtaca ggcaaatagc ccgatcttct tggcagaact
    21901 catgctcttc tcctaatttc atttttgaat ttggagtccg ggtcatgatg tataactatt
    21961 tcctgaccag accaaactgg cgctaagatt actcacgaaa aaaggattaa tcctaaagat
    22021 taggtgaaat aacacaaaag tttctgtaag tgagaacttg aggtttttta ttaacacatc
    22081 aggatcgcaa gttgatatca tgaaaagata aacattcaat gtttacaata gattgcgtga
    22141 cgtcttctgg ttaaatttat gtaataaaaa ttatgcggca aataaattgc cgcaacatat
    22201 tataccaaca ggaacataca aaaactcaac aacaaatatt cccaagcatg aatcaacccg
    22261 ggggttactt attctgaagc gagaaatttg tcgagataag gtacaacata aggaacagaa
    22321 gtctggaata taccattttc aatccagtaa agggtgtttg cccctgggcg taaattaaag
    22381 gcggtgagat atgcatcagc cgcttcccgg ttcatcccct tcatttcata aaccttgcca
    22441 agcaatacat aatttagcca ggacatttca agatcaatgc cagtatttat cgcctggtaa
    22501 gactcatctg ttttaccttt taccagagca ctgaccgctt ttatttgata tataatggac
    22561 aggttattca attccggtag tgtaacaatg ttatctattt ctgtgttcag tgctgctaat
    22621 tgtttttcat ctaaaggatg ttgagaatgg cgcacgatat caactaatgc tttttctgct
    22681 ctcgcgtagg taaattctgg ggatgattga acaatctcac ctaataattc actggcacgg
    22741 ttcaatgatt tatcatcgcc atgcagtaaa taatcatgtg cctgataaaa attagttaat
    22801 aacgcaccac gatgaggcaa aatttgctgg agcgtctcct gcattcgttg tggccacggt
    22861 tggtttaacg cttttgataa actctccagt aaatcatttt gaatcgccag ctgattaccg
    22921 ttagtgatga cataacgttt atccagcatg gttgaaccat ctgcattgtc taccaatttt
    22981 atcgacataa agcattgttg agcacggtat tggcgctgat taacaaacgc aatagataat
    23041 gttttaccgg aactgctcgg ttcatcaatg ttgtagttga ttttgtcatg caccataaag
    23101 gtggagaagg tgttaagtga tgtcgccacc aaatcaccca cgcctatcgc gtaagagagc
    23161 tgatacgggg aactccagct attacaactt ttatttacca tattaatgtc aatatcgcgt
    23221 ggattgagca aaatacgcga tttgctcatc ggaagacgtg tatcaagact tgaaaacgct
    23281 accagtgcta cacagatacc taaggacaac aggaaaaaaa accataccca aaaggtagtg
    23341 aatcgtttgc ttttaactgg ggattgttca ggtggcgttg cggtgttttg aatgttaaga
    23401 ctgtgggagg gagaatctgt ggcaggaacc gcctctggta tagggggagg cgaagatagc
    23461 attatttcct ctccctcttc ttcgctgtac cagataaccg gcaccattaa tttatagccg
    23521 cgctttggta cagtagcgat atagacagga ctatcttcat cattatcttt taatgactta
    23581 cgtagttctg agatactctg cgtcacaaca tgattggtga caatacttct cttccagaca
    23641 ttatcgataa gttcatccct gctaagtact tcgccactgt gttgagcaaa gaaaaccaga
    23701 aggtcgatta atctcggctc aagggtaagt tgacgcccat tgcggctaat ttggtttatg
    23761 gacggagtaa caagccattc gccaacgcga actacaggtt gttgcataat agaaactcat
    23821 tcgaaaaggg aatgatgcaa tgataattgc cacaacctat ttttaccatc tatagatggg
    23881 tttatttaca tattattggt gaatgcaaga cgttattttt accagccata aacttcttga
    23941 ttacatggta ttaagaaagg attttactga gaaccagaag taatattcct taccatcaaa
    24001 attcatcatc tttgccaaag aaaaatgttc agaaaataat ccatggaaaa ttgtccggag
    24061 cgcttactat tttaatggat tgttagtctt tgcatgagca agcggactga tacatttctc
    24121 tttgttctca ttcagaaaat ctcatcagtc ggcgttcaga ccggtgttgt gctttatgga
    24181 tgcgttacgc tcctgatgac gtcatttgac gttcaacaac atcacggggc cgcacgacat
    24241 ttcacgtcag ttagtgctat cgctcaggaa caaattttcc cgaattggga tatgcccgca
    24301 aattgctggt gatgtgggga gaatctggtt gagttcggga gaattgattt ggaggcagaa
    24361 cgcttaaatc gtggcgtcct gaaacgaaaa acggacctcc gtggaggtcc gtttatatga
    24421 atttggtgcc cggactcgga atcgaaccaa ggacacgggg attttcaatc ccctgctcta
    24481 ccgactgagc tatccgggca acggggcgca ttaaaccgta atctgtacat ctcgtcaacc
    24541 taatttcagt aaaagcgatt caactgctta agattgcggc aaatcgcttc ttttctgtgt
    24601 ttctgtcagg tcaatgcacc accctggcgg cagcgggcga agcgcaggat atcttccgcc
    24661 agccgatgtg cagtgtcgac atccgcctgg ctacgattca ccagcattcg gcttaaacag
    24721 ccttccagca ccagttccat ctgctttgct accatcgccg gatcgtcaac ttccagcgtg
    24781 gttaacagtt cgtgggtgaa atcgtaggcc gcgctttttt gctgatcggc cagttgatga
    24841 atagggtggc caggatcggg ataaaacgta caggcagcga taaatagaca gcccggatag
    24901 cggttgtttt taacgcactc cgataacgcc tgataacgtg ccagcagctt ttgttcggcg
    24961 gtttgcgttt cgtccagcat cagctgacga cgccagacat ctatctgttg gctaagataa
    25021 cgcagcgcat cgtagaggat tgcctctttg tctggccaga agcggcgcag ctcgtccagt
    25081 ggataatcca cacgttcagc aaccatctcc agcgtggtgt tggcaatccc ttgtaattct
    25141 aataatttca gggcttctcc cagtacatct tcacgttgca cgctattttc ctccgtcttt
    25201 cccactgcaa gtgtcgttca cggttggcga tcgcgcaaat gtgcgctgaa ggtttcagca
    25261 tccataaagc ccgtgacgcg tgcttgtgga tgctcctggc cttgtccgtc aaaaaagaga
    25321 attgtcggta ggccaaggac attaagatgc tttaacagcg ccacatcttg tgcgtcgtta
    25381 gcagtgacgt tggcctgaag taagaccgtg tctgctaacg ctttttgcac ctgcgggtcg
    25441 ctgaaggtgt atttctcaaa ctctttacag gcgacgcacc agtcggcata aagatctaac
    25501 atcaccggtt tgcctttggc ttcaacgagc gcctgattta actcatctac cgttttgatt
    25561 tgtgtaaagt tgagatgcgt ctgagtttgc gcggtatgcg tcgcaccaaa tgcccaatcc
    25621 tgaagtgggc gcacgctaac caatgccgct gccagcagaa taatttgcac aatacgcatc
    25681 cagccgcgtt tagcctgtag gctggtgata aaggcccagc caaagaatgc gacacccagc
    25741 gccgaccaca agcgtaatcc ccatacatca ccaatcactc gctccagcag gaagaccggc
    25801 agtgcgagga tcacaaaacc aaacgcggtt ttgacttgtt ccatccacgg gccgcttttc
    25861 ggcagcaagc ggttaccaaa gacggtaatt agcatcagcg gcaggcccat gcccaacgca
    25921 tagagataaa gcgtgccgcc gcccagccac atgttcccgc tttgggcgat atacagcaga
    25981 atcgcgctaa gcggtgcggt ggtgcatggt gaacagatca gtccggcaat cgcccccata
    26041 acaaacacac cgccaggtga gccgccctgt tggcgattgc tcatcaacgt gagacgtgtt
    26101 tgcagcgaag aggggagttg tagagtaaac aagccaaaca ttgacatcgc cagcaaggta
    26161 aagacgatgg cgaggccaat gagcacgtat gggtgctgta gcgccgcctg gaactgtaac
    26221 cctgcggcgg caactaccag acccagcgcc gtgtaagtca gtgccatccc ctgcacataa
    26281 ataaaggtca gcaacaatgc tctggcagtg gagagccgct gtttaccacc cagcacgatg
    26341 ccagaaatca gtgggtacat tggcagcacg catggcgtaa aggcgatacc aataccgatc
    26401 aacaacgccc agagcgcgga aaagggcaat tgcgcggtgt gctgctcttg ctgcgaaaca
    26461 gacacaggct gtgacgctgc gttgttggcg accacttcgc ttaacggaac ggttttggtt
    26521 tctggcggat aacagaaacc ggcatcagca cagccctggt aggtgacagt taacgtcgct
    26581 cccgcactcg cctggttgat ggttacggga agcgtcagcc gatcgcggta aatctcgctt
    26641 ttgccgtaaa actcatcttc atgccagacg ccttgcggca gctgcacgtc ggcaattttc
    26701 gcgtgttccg gcgtaatgcg gatctgttta cggtagaggt agtaaccgtc tttgatctgc
    26761 caggtcagat taaggtcatg ttggttttgc tgaaaatcaa aagtaaaggc ttgatccgcg
    26821 gggacaaatt gtgaacgtcc cggcgcgtcg aataatccgg caaaaacgga agtgctgcaa
    26881 agtagcagga tcagcgtaaa gatgcgttga gccatgagag gtaatctgtg tctccgtgtg
    26941 taacaggtaa aaccagaagt tccggggttt gatatggatg atgagacttc aggcattcca
    27001 gcagtgcctg ctggtgagat acggtagttt ttaaaatcat ctgcacttcg tattcttgct
    27061 ccagcttacc ttcccagtaa tagagagagg tagcgccggg gatcaaggtc gcgcaggccg
    27121 ccagtttttc cgccagcact ttggcagcta aatcctgggc tgtcgcttca tctggtgccg
    27181 tacatagcac cacgacagac gcggtattcg aacttttttc atcaagcata aacacctcgc
    27241 aagaacagat ggaaccgcaa gagagaggtc actatacaac ggacggggga aggatgttag
    27301 tcggcgggaa agaaagcggg gcgtgaacgc cccgcgattg atcattacag catgaagcta
    27361 cccagcacga agccgaagca aacggccagg gcaacaccca gagtacccgg gatgaagaac
    27421 ggatggttga agacgaattt accgatacgg gtagtacccg tgtcatccat ctgtaccgca
    27481 gcaaccagcg tcggataggt cggcagaatg aacagaccag acaccgcagc gaaagaagca
    27541 acagcggtca gcggtgaaac gttcagtgcc agagccatcg gcatcagtgc ttttgcggtt
    27601 gcagcctgag agtacagcag agcagaagca aagaagaaga tgacggccag cagccacgga
    27661 tgaccctgaa tcacttcacc agcggtatct ttgatccagt cgatgttgtt ggaaacgaaa
    27721 gtatcgccca gccacgcaac acccaggata caaatacagg cgctcatacc tgctttgaag
    27781 gtgctggagt tgaggatgtt gtcggtatcg actttacaga taacggtggt cagagttgca
    27841 acgctgagca tgatgatcag gattgcgttg gtggtgttca tcagcggttt ttcaaccaga
    27901 cccatgcttg ggctgttgat gattgcatag ataaccacgc caactacgcc cagcaggaac
    27961 agccaaacgg acgtttttgc accggatttg atttcaatct gcttttcacc gcgcagttca
    28021 accaagccct cttccagacg cttgcgataa atcggatcgt cagagagttt ggagttgaac
    28081 agcatagtga ccagaaagga catcaccaga accgccagca gggtggacgg gatgaccacg
    28141 gagagcagat ggaggtagct gatgccatga ccttccatca cggaagacat gtaaaccact
    28201 gccgctgaga ttggcgatgc ggtgatcgca atctgcgcgg ataccactgc agtagacagc
    28261 ggacggcaag gtttaacgcc ttgttccttc gcaacttcag cgataactgg cagtgtcgcc
    28321 agagagatgt tgccagtacc agcaaagatt gtcaggaaat aggtcacgat aggtgcgagg
    28381 atcgtgatgt atttcgggtt acggcgcagc agcttttctg tctgatgaac cagatagtcc
    28441 agaccgccag caacctgcat ggcagaaata gcggcgataa ccgccatgat aatggaaatg
    28501 acatcgaacg ggatgttacc gggtttaacg ccaatagcgg caagaaccag cacccccaat
    28561 ccgcctgcaa aaccaatacc tattcccccc aatctggcgc ccaagaagat cgccagcaaa
    28621 actatgatga gttctacaac tagcatatta gccttccttg ttttttaaca agttgatatt
    28681 agattgttgt ttttaagtta ctgctcacaa gaaaaaaggc acgtcatctg acgtgccttc
    28741 tttatttgta ctaccctgta cgattactgt tcgctttcat cagtatagcg ttttgctttg
    28801 taagccgggt gcatcagatt ctgtacggag aaaatatcgt caagttccgc ttcagtcaac
    28861 agaccgcgtt ccagaacgac ttcacgtaca ctcttaccgg tttcggcaca gattttaccc
    28921 acgatgtcac cgttgtggtg accgatgaac gggttcaggt aagtaacgat accgatagag
    28981 ttgtaaacgt aaccttcgca cacttctttg ttagcagtga tgccgttaat gcatttttcc
    29041 agcaggttgt agcaagcgtt ggtcagaatg tgaacggatt cgaacatagc ctggccaatg
    29101 accggctcca taacgttcaa ctgcagctga cctgcttctg ctgccatggt aacagtggtg
    29161 tcgttaccga tgactttgaa gcatacctgg ttaaccactt ccggaacaac cgggtttact
    29221 ttagctggca tgatggaaga gcccgcctgc agttccggca ggttgatctc gttcaggccg
    29281 gcacgtgggc cagaagagag caagcgcagg tcgttacaga ttttggacat cttcacagcc
    29341 aggcgtttca gcgcgccgtg aaccataaca taagcgccgc agtcagaggt cgcttcgatc
    29401 aggtcttcag ccggtacgca tgggaagcca gtgacttcag ccagtttttt cactgccagc
    29461 ggagagtact ctttcggcgt gttcagacca gtaccgattg ctgttgcgcc aaggttaact
    29521 tccagcagca gttcagcggt acgttggata tttttcactt cttctttcag caggatgctg
    29581 aaagcgcgga attcctgacc gagggtcatc ggtactgcgt cctgcagctg ggtacgaccc
    29641 attttcagga tgtcctggaa ttcgatagct ttacgttcaa agccttcacg cagttggtta
    29701 atcgcatcta ccagcttaat cagggaagag taaactgcga tacggaaacc ggtcgggtag
    29761 gcgtcgttag tggactgaca tttgttaaca tggtcgttcg ggttcaggta ctgatattca
    29821 cctttctggt gacccatcag ttccagaccg atattggcca gcacttcgtt ggtgttcatg
    29881 tttacggaag tacctgcgcc gccctggtag acgtctaccg ggaactgatc catgcatttt
    29941 ccgttgttca ggacttcatc acatgcggca atgatggcat tcgctacact tttaggaatg
    30001 gtttgcagct ctttgtttgc catagctgcg gcttttttaa ccattaccat accgcgaaca
    30061 aattcaggaa tatcactgat tttgttgttg ctgatataga agttttcaat cgctctcaga
    30121 gtgtgaacac catagtaggc atcagctgga acttccctgg tacccaacag atcttcttcg
    30181 atacgaatgt tgtttgacat gtgaaccttc tttttcaagc tgccaatgat ttgctttaaa
    30241 cacacagaat atatgtggtt tcgaatgttt ttcgaccgac gattatcccc tgcatcgacc
    30301 gaatacccga gatcatatgc tgcttgagga tttctaccgt aatctggatc actttaagtg
    30361 tcggttttta ccccttaatt attaatttgt gaaatagatc accgctttgg gattactacc
    30421 aaaaatagtt gcgcaaacat cttgaaattt tgctaatgac cacaatataa gctgaacgcg
    30481 attcgcaacc cattcaggta gccgggttta accggctgct attacaggag aaacctttgc
    30541 gctggttacc ttttattgcc attttccttt atgtctatat tgagatttca atctttattc
    30601 aggttgccca tgtattgggg gtattgctga ccctcgtgct ggttatattc acgtcagtta
    30661 tcggtatgtc actggtacgt aaccagggct ttaagaattt cgtgctgatg cagcaaaaaa
    30721 tggcggcggg tgaaaaccca gcggcggaga tgattaaaag tgtttcgctg atcattgccg
    30781 gtttgctgct tttattaccg ggctttttta ccgacttcct cggtcttctg cttttattgc
    30841 cgccggtgca aaagcatctg acagtgaagt tgatgccgca tttgcgcttt tctcgcatgc
    30901 ctggcggcgg ttttagcgcc gggaccggtg gcggtaatac ttttgatggt gagtaccagc
    30961 gaaaggatga tgaccgcgac cgccttgatc ataaagacga tcgccaggat taatgtcgaa
    31021 acgccggatt atgtggttat gccattttcc ggcgtttttc gttttggcag aaacagccat
    31081 aaccccgcca gcataatcag cgcatagaga cttttccagc cgaccattgc cagtaacaga
    31141 acgcataaca gcccgccaac caccgccagt agtcgataac gtccttgcaa taatttacag
    31201 cctgccagca tgcataacag ataaatcata ataaagatgc cattggcata aataataaga
    31261 gcgtccagat tgatctctaa agcatgaatc accaaagtgc tcaccacgca gcagccgagc
    31321 accgcattga gggcattatt cgggatatgg cgagaagaga ggcgtgccag gtagtggtca
    31381 ggattatgtt gcgcctgcga ccagaccagg cgggcgaagc tctgtatata aatgttgaga
    31441 ctggcaaagc aggccagata gccaattacg caggcaatcc ataacgctcc tacaccgaac
    31501 agttgcacta caatttttgg aagcgatgct gccgcggcca tttgttcacc ataggcgtcg
    31561 aagtgtaaga cgactaccgt acagccccag tagactaatc ctgccagcag cagaccaatc
    31621 atcaaagcac gaggaaaatc acgttctgga tttttaaatt ccgaggcaag atgggcaaat
    31681 gcttccagac cgacaaaaca ccagaacatc actgataacg cagcaaataa cccggtaagt
    31741 tcgatatttc ctggcgcggg gaaggggata ctcgcaggtt tgatatcgcc cgcccaccag
    31801 atagcgacaa tcagtgcgac gataagtccg gcaataacgg tttgtagatt tgcactggaa
    31861 ctggcaccgc gagtaccgat ataccacacc aacgccagcg taccgagttc tgccaacaac
    31921 agttgccagc tatgccagcc aaacatcgcc tggccgaacc cggcggcaat ttgtagcgcg
    31981 gcaggcaaac ccacgggaat gaccgataaa aacagccagc cggtgactcg ctcaagccgc
    32041 gaaccaaacg ccataccgac gaagtgcgcg acgccgcctg cactgggata gtggcgaccc
    32101 agaatcgcaa acacaatcgc aatcgggaac actaagataa tcaaaacggg ccacgcccac
    32161 aggctgttat tgcccgctac cagcgcagct aacgcaggaa cggcaaacac gccagtgcct
    32221 aataatgacg tcgatagcag tccaatgccc tgggccagcc ccagttcttg tttgagtcca
    32281 ctcatgggtt gatatccgat tgcgcccaaa ttttgggcaa ctgcgtagat tttcgatggt
    32341 agcacaatca gattcgctta tgacggcgat gaagaaattg cgatgaaatg tgaggtgaat
    32401 cagggttttc acccgatttt gtgctgatca gaattttttt tctttttccc ccttgaaggg
    32461 gcgaatcctc atccccattt ctctggtcac cagccgggaa accacgtaag ctccggcgtc
    32521 acccataaca gatacggact ttctcaaagg agagttatca atgaatattc gtccattgca
    32581 tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa tctgctggcg gcatcgttct
    32641 gaccggctct gcagcggcta aatccacccg cggcgaagtg ctggctgtcg gcaatggccg
    32701 tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa gttggcgaca tcgttatttt
    32761 caacgatggc tacggtgtga aatctgagaa gatcgacaat gaagaagtgt tgatcatgtc
    32821 cgaaagcgac attctggcaa ttgttgaagc gtaatcctcg cacgacactg aacatacgaa
    32881 tttaaggaat aaagataatg gcagctaaag acgtaaaatt cggtaacgac gctcgtgtga
    32941 aaatgctgcg cggcgtaaac gtactggcag atgcagtgaa agttaccctc ggtccgaaag
    33001 gccgtaacgt agttctggat aaatctttcg gtgcaccgac catcaccaaa gatggtgttt
    33061 ccgttgctcg tgaaatcgaa ctggaagaca agttcgaaaa catgggtgcg cagatggtga
    33121 aagaagttgc ctctaaagcg aacgacgctg caggcgacgg taccaccact gcaactgtac
    33181 tggctcaggc tatcatcact gagggtctga aagctgttgc tgcgggcatg aacccgatgg
    33241 acctgaaacg tggtatcgac aaagccgtta ccgctgcagt tgaagaactg aaagcgctgt
    33301 ccgtaccgtg ctctgactct aaagcgattg ctcaggttgg taccatctcc gctaactccg
    33361 acgaaaccgt aggtaaactg atcgctgaag cgatggacaa agtcggtaaa gaaggcgtta
    33421 tcaccgttga agacggtacc ggtctgcagg acgaactgga cgtggttgaa ggtatgcagt
    33481 tcgaccgtgg ctacctgtct ccttacttca tcaacaagcc ggaaactggc gcagtagaac
    33541 tggaaagccc gttcatcctg ctggctgaca agaaaatctc taacatccgc gaaatgctgc
    33601 cggttctgga agctgttgcc aaagcaggca aaccgctgct gatcatcgct gaagatgttg
    33661 aaggcgaagc gctggcaact ctggttgtta acaccatgcg tggcatcgtg aaagttgctg
    33721 cggttaaagc tccgggcttc ggcgatcgtc gtaaagctat gctgcaggat atcgcaaccc
    33781 tgactggcgg taccgtaatc tctgaagaga tcggtatgga gctggaaaaa gcaaccctgg
    33841 aagacctggg tcaggctaaa cgtgttgtga tcaacaaaga caccaccact atcatcgatg
    33901 gcgtgggtga agaagctgca atccagggcc gtgttgctca gatccgtcag cagattgaag
    33961 aagcaacttc tgactacgac cgtgaaaaac tgcaggaacg cgtagcgaaa ctggcaggcg
    34021 gcgttgcagt tatcaaagta ggtgctgcta ccgaagttga aatgaaagag aaaaaagcac
    34081 gcgttgaaga cgccctgcac gcgacccgtg ctgcggtaga agaaggcgtg gttgctggtg
    34141 gtggtgttgc gctgatccgc gtagcgtcta aactggctga cctgcgtggt cagaacgaag
    34201 accagaacgt gggtatcaaa gttgcactgc gtgcaatgga agctccgctg cgtcagatcg
    34261 tcctgaactg cggcgaagaa ccgtctgttg ttgctaacac cgttaaaggc ggcgacggca
    34321 actacggtta caacgcagca accgaagaat acggcaacat gatcgacatg ggtatcctgg
    34381 acccaaccaa agtaacccgt tctgctctgc agtacgcggc ttctgtggct ggcctgatga
    34441 tcaccaccga gtgcatggtt accgacctgc cgaaaaatga tgcagctgac ttaggcgctg
    34501 ctggcggtat gggcggcatg ggtggcatgg gcggaatgat gtaattgccc tgcacctcgc
    34561 agaaataaac aaacccccgg gcagaaatgt ctgggggttt ttcttttggt catctttcta
    34621 gtataagatt cagacacgga cgacgcgagt ggcgtccagc tcattgatta tggggaataa
    34681 catgcacgta aaatacttag cagggattgt cggtgccgcg ctactgatgg cgggttgtag
    34741 ctccagcaac gaattgagtg ctgccggtca gagtgtacgc attgtggacg agcagccagg
    34801 cgcagagtgc cagctgattg gtactgcgac aggtaagcaa agcaactggc tttccgggca
    34861 acacggagaa gagggcggtt ctatgcgcgg cgcagcaaac gatctgcgca accaggcggc
    34921 tgcaatgggc ggtaacgtga tttatggcat cagcagcccg tcgcagggaa tgttgtccag
    34981 ttttgtcccg acggatagcc agattatcgg tcaggtttat aagtgcccga actgatgtga
    35041 tggtgacaaa cgtataattg ccagaggcgc ttcgcttatc aggcctacat aaggcattgc
    35101 aactgattga atgcttgaga atttgtaggc cggataaggc gtttacgccg catccggcat
    35161 gaccaacgat cacgttatca gtcatttcag gctggatggc tccagcctcg cattagcaaa
    35221 gatgagatta tttcgcctgt ggtgcagttt tgctggtgga ttgaacacgt aagtgatgca
    35281 gcgcgcgtaa gcattgatcc tgggttagcg gttgggcgtt attcgccgct acggtgcgaa
    35341 catagacctg acatggcaca cccgcgagtt ttttcagtcg tgggctgaaa tcaagcattc
    35401 ggcgagtgat cgcctctatt tctttacccg gcgttgcttc acgcgttttc accactgcgc
    35461 cgctaaattg ttctttgcca ctttcatctt taaagcggta aagcaccgcc agataatggt
    35521 cgaacaggtt atgtttccat tccggttggg aataggtgtc atattccaga ttgccattgt
    35581 cctggcagtc aacgtcgtat aacggcagga aatctaacag tgaggtaata cgtaatgcca
    35641 gttcacgcat ttccgcatta ttaattgcat gaatgattgc tcgcgccagc atttcaatct
    35701 gcaattcatt gacgactaac tcacatttgc taccgtcatg tagcgttaat acgaaggtaa
    35761 gattttcgcc ttgattatcg gtaagttcca gcgtgttaac ccgacgatta atatcggcat
    35821 ttttaagctc atcaaccaga atttctggga tattttctgc cattttctta atgactttat
    35881 cgcgagcttg ctcatactga aggcgtgcag cagcatccag tttatgcttt tgatgcagac
    35941 gactttccag cgcaatcagc aggtcgcgca gttccatgac agacataaag aacaacgatt
    36001 ctttattgcg aggttcttta atttttagcg ccagtgcaat gaaattatta ctcttgcgaa
    36061 taacaccagt attaacaccc ttgatgctta tggccatgta ggttctccct aaccatttct
    36121 caattaaata attaatttta atttataagc cagataaatg ggcttggtag taatagttgt
    36181 taaaataaca taaatagccg tactcactct atataaacag aaaagaaagg ttaattattg
    36241 gtgttagtta tataaagagt aacaacagca atgcatatga ataattaaag gttatgcaac
    36301 gtgcaaagat taaatttccc agtttaatga caggttgctg tacttgcgtt tgttgctctt
    36361 gccggatgcg gcgtaaacgc cttatccggc ctacagttca ttgcaaatcc attcaatttc
    36421 aatgcacgat gtaggcctga taagcgtagc gcatcaggca attttaatgt ttgacttccc
    36481 tgtttaatca gttactgctg gcgtagctgg agatccagcg gcgttttgct gggttcgccg
    36541 ccaatttctc gcgccagttt cggcaccaga tatcccgaca ccagtgtcag caactcacgc
    36601 ataatctgcc gtgcttcgtc atcactcacc ataaaatgcg ccgcgccctg tactttatcg
    36661 agcacatgca ggtaatacgg catcacgccg gcatcgaaca acgcattact caggtttgcc
    36721 agcgtttgtg cgttatcgtt cacaccacgt aacagaacgc tctggttcag caaagtaacg
    36781 cccgcccggc gcaacttagc catcgcctga cggaatgttt catctatctc attggcatgg
    36841 ttgatgtgat tcaccagcaa gatttgcagc gtagaatgtg aaaagcgttc aaccagcgca
    36901 tctgtgatgc gtgccgggat cacaattggc agacgactgt gaatccgcag acgtttgata
    36961 tgcgggatgg cttccagttg tgtgagcaac cagtccagct cgtgatcttt cgccatcagc
    37021 ggatcgccgc cggagaaaat catctcgtcc agttccggat gcgcagcaac atactcaagt
    37081 gcagtttgcc agttacgctt gttgccctga ttttcggcat aagggaagtg gcgacggaag
    37141 caataacggc aatttaccgc gcagccgcct ttgaccagca aaagcgcccg gttgtggtat
    37201 ttatgcaaca aaccaggcac tacgctgtgc tgttcttcca gcgggtcggt ggagaatccg
    37261 ggcgcgatgg caaactcatc ttgcgaggta agtacctgac gcaaaagcgg atcgtccgga
    37321 ttgcctttct ccatgcgatc gataaatgag cggggcacac gcagggcaaa cagctttttg
    37381 gcgttgcgtc cggccgacag tttttcgtcc gcgtctatat tcaaaagacg cagaagttca
    37441 tcaggatcgg tcacaacatc ggcaagttgc gttaaccaat cttctctgga tggggtattt
    37501 agggttacaa tatgcgccat tttgtggctt agctaccaat taacaaattt cagagggcct
    37561 tatggcaacg tactatagca acgattttcg tgctggtctt aaaatcatgt tagacggcga
    37621 accttacgcg gttgaagcga gtgaattcgt aaaaccgggt aaaggccagg catttgctcg
    37681 cgttaaactg cgtcgtctgc tgaccggtac tcgcgtagaa aaaaccttca aatctactga
    37741 ttccgctgaa ggcgctgatg ttgtcgatat gaacctgact tacctgtaca acgacggtga
    37801 gttctggcac ttcatgaaca acgaaacttt cgagcagctg tctgctgacg caaaagcaat
    37861 tggcgacaac gctaaatggc tgctggatca ggcagagtgt atcgtaactc tgtggaatgg
    37921 tcagccgatc tccgttactc cgccgaactt cgttgaactg gaaatcgttg ataccgatcc
    37981 gggcctgaaa ggtgataccg caggtactgg cggcaaaccg gctaccctgt ctactggcgc
    38041 agtggttaaa gttccgctgt tcgtacaaat cggcgaagtc atcaaagtgg atacccgctc
    38101 tggtgaatac gtctctcgcg tgaagtaatg cggttgtggt gcggcctgca ggctgcacca
    38161 tcacttattc aggtcagaga tgatgaaacg ccttatcgtt cttgttttgc ttgccagcac
    38221 gctgctcacg ggctgtaaca ccgctcgcgg tttcggcgaa gacatcaaac atctcggcaa
    38281 ctccatctcc cgcgctgcca gctaattttt cttctcttcc gaaaaatcat cagatttcca
    38341 tcatttttgg cgatgttgtc tattattaat ttgctatagg caaacataaa taacattacc
    38401 taaaaggaag acgttatggt gaagaagaca attgcagcga tcttttctgt tctggtgctt
    38461 tcaacagtat taactgcctg caacaccacg cgtggcgttg gtgaagacat ttctgatggc
    38521 ggtaacgcga tttctggcgc agcaacgaaa gcgcagcaat aagcaataac ggtacgacag
    38581 cagtgtcgtg ccgtttgttt tttctgcgat agtcataaag gtaatagttg aaattcccct
    38641 gccacctggc aaaatatccg ttcaaccatc agctttgcag gacgacctgc aaacgcctct
    38701 tttcaccggg gacggcccca aatttccgga gcctgatatg tcctggatta tcttagttat
    38761 tgctggtctg ctggaagtgg tatgggccgt tggcctgaaa tatacccacg gctttagccg
    38821 tttgacgccg agtgttatta ccgtgacggc gatgatagtc agtatggcgc tacttgcctg
    38881 ggcgatgaaa tcgttaccgg tagggacggc ttatgccgtg tggacgggta ttggcgcagt
    38941 cggcgcggcc atcaccggca ttgtgctgct cggtgagtcc gctaacccga tgcgtctggc
    39001 gagtctggcg ttaatcgtat tggggattat tggtctgaaa ctcagcactc actaactact
    39061 gggctgctgt acccaaataa acttactgac atcaaaccct tcccgggtcg cgactgccag
    39121 catctcctgt ttcacttcgt cagaaatggt tggcgtgcgg gagagtatcc acaggtagtc
    39181 gcggtccggg ccgcaaacca gcgcatggcg gtattcccga tcgagtgcaa taacgttata
    39241 accgccatag aaaggaccaa agaatgacac tttcagcgca gcgcgagttg atgcgccggt
    39301 aaagtacgct ttcccttcac tctgctgcca catttctctg tcagggttat agcctttatt
    39361 aatgacattc aggccgccat catccctcag gctgtatgtt gctgtgactt tttccagtcc
    39421 acgttcaaag cggtgatcaa aacgggcaat ctcataccag gtaccgagat agcgtttagc
    39481 gtcgaaatta ttcactacgg tcacgccacg cggcggcgta ggagaactgc aggcaacgac
    39541 cagaaatgca gctgtcgctg cggcaacgag agggagcagg cgcataaatg tttccttact
    39601 ggtttttttc taagtgtaga tgacagcaag gaaaagcgca gaaaaggtcc gaaaattcgg
    39661 acccgatgaa attttactgc agagcgttga gaatctgcca ggcggcggcg actctcgctg
    39721 gattgggata gtttttgtta gccagcatca cgatacccag ctctttttct ggaataaacg
    39781 cgacatagct accaaatccg ccggtcgccc ccgttttatg cacccatgat gcgcgtactg
    39841 caggagttgg gggcgtaatc gcttttacgg ggcgtgctgc cagtgcaatt ttattgtcac
    39901 tgccgttaac gatgatgtca ggatttaccg gccagtccag catttcccag cccagaccct
    39961 gatacatatc gccggtttgc cagtagcgag attgtgccag ttgtatccct tgttgaagtg
    40021 ttttgtcgtt gatatcacgg tgattcatat tgcttcgtac ccagcaggcc atatcttcaa
    40081 tggtcgactt cacaccataa gcttcagcat ctaacgcccc tggcgaaaca tgcactgcct
    40141 taccttcgcg atatccccag gcgtaattct tttcttctgc gggcggtaca ttaatccacg
    40201 tatggttgag tttgagtggc tggaagacac gagtttgcat cgcctgctca aaactcaaac
    40261 cagacggctt cacagccagt gcgccgaaca aaccgatact ggagttggca tacagacgtt
    40321 gtgttcctgg agcccatgca ggctgccagt tttgatagaa gcgcagcaag tcgcttgagg
    40381 atttcacctc atccggcacc tgcaatggca ggccgccagc ggtgtaggtt gcgagatgta
    40441 atagtgtgat cccgttccac tgtttagcgg taagttcagg ccagtatttt gttgcgggat
    40501 cgcttaactt gatttcccct cgagcaatag cgtcgccacc aagcacgccc gtaaatgttt
    40561 tgctgaccga acctaactca aacaacgttt gctgtgtgac gggctgcttt ttggcgatgt
    40621 ccgcatagcc ccaggtaaag taataaggtt taccctgata aattaccgcc accgccatac
    40681 cggggatctt ttgttgctct ataagcgggg taattgtgcg atgcacaata tcgttgattt
    40741 tttggggggc agcaaatgtg gagcaagagg cggtaattaa taaggcgcag agcgtcgttt
    40801 tgaacatagg gtctggtttc catacaaaac ggcccgccat aggcgggccg gatttacatt
    40861 ggcgatgcgt tagattgtaa cgacaccaat cagcgtgaca actgtcagga tagcagccag
    40921 accgtagaaa acccatttgc ccgcaggtac gtggattttc agatcgtgca tcgcgtggtg
    40981 catacggtgt aaaccacacc acagcggcag aacgatcatc aggaacagga atacgcgacc
    41041 aatgaagctc tgcgcgaacg ccagaacgcg ctcgtagctc agtgcatcac ccggaaacag
    41101 ccccagcggc agcagaatac ccaccagcag gatcatcacc ggcgcaataa ttgcgctcca
    41161 cataccaccg gccccgaaga ggccccagaa taccggttcg tcagaacgct ttggatttgg
    41221 attgatcatc tcaggctcct taccagtaca gggcaacaaa caggattacg atggtggcaa
    41281 ccacagttac cgcccagaga cttttgataa ttggctctgg acccattttt tcgtctttta
    41341 caatgatatt ggccgctttc ggtgccagtt caaaccaggt tttggtgtgc agcagagctg
    41401 ccgccagagt gatcaggtta atgatcacaa taaccgggtt ttgtaaaaag tcgacgaatc
    41461 ccgcccaggc ttccgggcca tttttcaggg caaacagccc gaaaatcagt tcaatgctga
    41521 accacacagc cggaaccgcc gtgccttcgc gcagcatgta aaagcgataa aacggcaatt
    41581 ttttccacca agtggacgtc attggccgta catacggttt acgtttagtc gtcatgttgc
    41641 actccttagc gtggtttcag ggtcgcgata agaaagtctt tcgaactttc tactttgccc
    41701 tgctgaatgg ccgcagccgg atcgacgtgt ttcgggcaga cttcggagca atagcccacg
    41761 aaagtacagc tccatacgcc gttctggctg ttcaactgcg ccatacgctc cttcttaccg
    41821 tggtcgcggc tatcttcgtt ataacgatgc gccagcgtaa tggcagccgg accgatgaac
    41881 tctgggttca ggccaaactg cgggcacgcg gcgtagcaca gaccacagtt gatgcaaccg
    41941 gagaactggt gatacttcgc catctgcgcc ggggtctgga tgttagtacc ctgatccgcg
    42001 gtgcgggagt tgccgatgat gtacggtttg atcgcttcca gactttcgat gaagtgggtc
    42061 atatcgacca ccagatcgcg ttcaatcggg aagttagcta acgcttcaac cttcataccg
    42121 tcggtgtaat cacgcaggaa ggttttacat gccagttttg gcacgttgtt aaccatcatg
    42181 ccgcaggaac cacaaatcgc catacggcag gaccagcggt agctcaggtc cggtgccagg
    42241 ttgtctttga tgtagcccag cgcatccagt aatgaggtag ttgcgtcata aggcacttca
    42301 tagaatgcgc tatgcggtgc ggtatcgact tccgggttat agcgcaccac ctcaattttc
    42361 aggtttttca tctcagccat tcgccttctc cttcttattg gctgcttccg ccttatcggc
    42421 tgcatccgct tcgccaccgt aaacgcgttt agctggcggc agcgtagtaa tcttcacgtc
    42481 gctgtactcc aggcgagtcg tgccatcagc atcgcggaag gcgagggtgt gtttgaggaa
    42541 gttgacgtcg tcacgctcgg tgcaaccttc gtccagacgc tggtgtgcgc cgcgggactc
    42601 tttacgtgcc attgcggagt gcgccataca ttcagcaacg ttcagaccgt ggcccagttc
    42661 aatggtgtag agcaggtcgg tgttgaacac gctggaagtg tcggtgatgc gcacgcgctt
    42721 gaagcgttcc tgcagctcag ccagcttgtc gatggttttc tgcatcagtt ccggcgtacg
    42781 gtagataccg cagccttctt ccattgccag gcccatttcg tcgcggatct tcgcccagtt
    42841 ttcgccgcca tcctggttaa ccagatcttt cagacgttgt tcaacgccag ctgcctgcgc
    42901 ttcaattgcc gcttcgttgc cattaccggc agttgctgca cgctctgtcg cttgttcacc
    42961 ggccagacgg ccgaagacca ccagttccgc cagggagttg gagcccagac ggtttgcacc
    43021 gtgcagacca acagaggaac attcacccac ggcgaacaga cctttaatgc gggtttcaca
    43081 gttctgatcg gtttcgatac cgcccatggt gtagtgtgcg gtcggacgta ccggaatcgg
    43141 ttctttaacc ggatcgacgc caacgtacgc tttcgccagt tcgcagatga acggcagacg
    43201 ttcatgcagt tttttctcgc cgaggtgacg caggtcgaga taaaccacat caccacgcgg
    43261 cgtggagatg gtgttgcctt tacgccattc gtgccagaag gcctgagaaa ctttgtcgcg
    43321 tggacccagt tccatatatt tgtttttcgg ctcgcccagc ggagtttccg ggcccatgcc
    43381 gtaatcttgc agataacggt agccattttt gttgaccaga ataccgcctt caccacggca
    43441 gccttcggtc atcaggatac cggaacctgg cagaccggtt gggtgatact gaacgaattc
    43501 catgtcacgc agcggaacgc cgtggcttag cgccataccc ataccgtcac cggtaacgat
    43561 gccgccgttg gtgttgtaac gataaacgcg acccgcaccg ccggtagcca taacgaccgc
    43621 gttagcacgg atctgcacca gcgtgccttc catcatgttc attgctacca ggccgcgaac
    43681 atgaccatca tcaaccagaa tatccagcac gaaatgttcg tcaaaacgct ggatctgcgg
    43741 gaattgcaga gaggtctgga acagcgtgtg cagcatatgg aagccggtct tatcggcggc
    43801 gaaccaggta cgctcgattt tcatgccgcc gaagcgacgt acgttgacgc taccatccgg
    43861 gcgacggctc catgggcacc cccacagttc cagttgggtc atttcggttg ggcagtggtg
    43921 gacgaaataa tccacgacat cctgctcaca caaccagtcg ccacccgcta ctgtatcgtg
    43981 aaagtgatat tcgaagctgt catgatcctg cgcgacagcg gcggagcccc cttctgcagc
    44041 aacggtatgg ctacgcatcg ggtatacttt tgagattagt gcgatttttg catttggatt
    44101 tgcctgcgcg gcagcaattg cagcacgtaa tcccgcgcca ccggcgccta caatggcaag
    44161 atcggcttga aaggtttgca cgacattcct ccagattgtt tttatcccac agccccgtac
    44221 ttcagggtaa gtgcctgaaa gttacggtct gcgaacgcta ttccactgct cctttatagg
    44281 tacaacagta tagtctgatg gataagtctg aaatttgacg agatcgattt ttttagtgcg
    44341 caagagggct aaattatcac tgaagatgat taatttaatt actaaaccat cagatcgtgc
    44401 tttttttagt cactgcccac cgttgtttga ttcctgcgta aaatttgtct cgccgtcgcg
    44461 ttgcgagtag acttcgtgcc cttgtcaaaa actggagatt taactatgag cgaaacggca
    44521 tcctggcagc cgagcgcatc cattcctaac ttattaaaac gcgcggcgat tatggcggag
    44581 atccgtcgtt tctttgccga tcgtggagtg ctggaggtgg agacgccttg tatgagccag
    44641 gcgacggtaa ccgatattca tttggtcccg tttgagacac gtttcgttgg ccccgggcat
    44701 tcgcagggga tgaatctctg gttaatgacc agcccggaat accatatgaa acgcctgctg
    44761 gttgccggtt gtgggccggt attccagctg tgccgcagtt tccgtaatga agagatgggg
    44821 cgttatcaca accctgagtt cactatgctg gagtggtatc gaccgcacta tgatatgtac
    44881 cggttgatga acgaggtgga cgatctctta caacaggtgc tggactgccc tgcagcagaa
    44941 agcctttctt atcaacaagc tttcttgcgt tatctggaaa ttgacccact ctctgccgac
    45001 aaaacgcaac tgcgggaagt ggcagcgaaa ctggatttga gcaatgttgc tgataccgaa
    45061 gaagaccgcg acacgttgct acaattgctg tttacctttg gcgtagagcc aaatattggt
    45121 aaagaaaaac cgacctttgt gtaccacttt ccagccagcc aggcatcact ggcgcaaatc
    45181 agtaccgaag atcatcgggt cgctgaacgc tttgaggttt attataaagg tattgagctg
    45241 gcgaatggtt tccatgaatt gacggatgcc cgtgagcagc aacaacgctt tgaacaagat
    45301 aaccgtaagc gcgcggcgcg cggtttgccg cagcacccca ttgaccagaa tctgattgac
    45361 gccttgaaag tcggtatgcc tgactgttcc ggcgtggcat taggtgttga tcgtctggtg
    45421 atgttggcgc tgggcgcgga gacactggct gaagtcatcg cctttagcgt tgaccgggca
    45481 taattctgaa aaattactga attaacagcg aagaatggcg taataagcgc cattctgtag
    45541 catatgtttt catttttccg ctaaaggcca caatttaaga ctggatattt gatatcatcc
    45601 aggtatcaat tctgtattgg tttttacgct gcctgctcaa tgttgcgcag tgcagttcag
    45661 tgggcacgtg ttatacacgc gctgaaatga aggatggttt catgcctcac acgataaaaa
    45721 aaatgagtct gataggactc atattgatga tctttacttc cgtatttggt tttgccaata
    45781 gcccatcggc ttattactta atgggttata gtgcgattcc cttttatata ttttctgcat
    45841 tgttattctt tattccattc gccttaatga tggctgaaat gggagctgct tatcgcaaag
    45901 aagaaggtgg tatctattcc tggatgaata atagtgtcgg accacgtttt gccttcattg
    45961 gtacgtttat gtggttttcc tcttatatca tctggatggt gagtacctcc gcgaaagttt
    46021 gggtaccgtt ctcaacattc ctctatggta gcgacatgac ccagcactgg cgtattgccg
    46081 gactggagcc tacgcaggtg gttggtctgc tggcagtggc atggatgatt ctggtcaccg
    46141 tcgttgcttc taaggggatt aataaaattg cccgcattac tgcggtgggc ggtattgcag
    46201 taatgtgtct gaatttagta ttgctgttag taagcattac tattttgtta ttaaatggtg
    46261 ggcatttcgc gcaggatatt aatttccttg catcaccgaa cccgggttat cagtccggtc
    46321 tggcaatgct atcgtttgtg gtatttgcca tttttgccta tggcggaatt gaagcggttg
    46381 gtggtctggt cgataaaacg gaaaatccag aaaagaactt tgccaaaggt attgtttttg
    46441 ccgctattgt tatttcaatc ggttattcgc tggcaatatt tttatggggc gtcagcacaa
    46501 actggcagca ggtattaagt aatggttccg ttaacctcgg caatattacc tatgtgctga
    46561 tgaagagcct tgggatgacg ctgggtaatg cactgcattt gtcacctgaa gcgtcattgt
    46621 cgctgggcgt atggtttgcg cgtattactg gactttcgat gttcctcgcc tataccggtg
    46681 cgttctttac gctttgctat tcaccgttga aagccatcat ccaggggacg ccgaaagcat
    46741 tgtggccgga accgatgacg cgcctgaatg cgatggggat gccgtctatc gccatgtgga
    46801 tgcagtgcgg gttggttact atcttcattc tgctggtttc gtttggtggc ggtaccgcat
    46861 cggcgttctt taacaagctg acgctgatgg cgaacgtgtc tatgacgctt ccttacctgt
    46921 tcctcgcgct ggctttccca ttctttaaag cacgtcagga tctcgacaga ccatttgtga
    46981 ttttcaaaac gcgtatgtcg gcaatgatcg cgacggtggt tgtcgtactg gtggtgacat
    47041 ttgcgaacgt cttcaccatc attcaacctg tggttgaagc tggagactgg gacagcacat
    47101 tgtggatgat tggcggccct gtcttcttct cgctgttagc gatggcgatt taccagaact
    47161 attgcagtcg catggcgaat aaacctgagt tagctctcga ctgatcatat gaaatcctgc
    47221 aactcatcgt ctctccggcg atgagttgtc aggaagtaat gcgtatggat gatactctcc
    47281 agggccggtg ataactgaag atgatgttaa gctgctaaat gagccacgtt gacatcattt
    47341 ggcagcttca tgcattacaa acttcccgcc tgacgacctt tgcccgcaga agttaatgtt
    47401 ctccccgttt gtttaccatt caggctttcc agacgcatct ggaagggcgg gaatggcata
    47461 tcgataccgt gggcatggaa gcctgccaga atcagctggt ggatctcatg gcgtagcggc
    47521 atacggtgac ccatctcagc ggcgtaaata cgcagctcga aaatctgaat cccttgttgc
    47581 agatccacca ggaagacttc cggtgccggg ttgtcgatca ccagcgagca gcgacgcgct
    47641 gcggtgagca ggatttccgt cacttcttca ctgttggcat cggcaggggc cggaatcgtc
    47701 aacaccacgc gcgtgaccga gtcagagagc gaccagttga taaactgctc ggtaataaac
    47761 gccttgttcg gcacgattat ctctttacgg tcccagtcgc tgatggtggt tgcgcgggta
    47821 ttaattttcg tcacgctacc agtaagatcg cggatcgtta ccgtatcgcc aatgcgaatc
    47881 ggtttttcga acaggatgat caggccagag ataaagttgg cgaaaatttc ctgtaaacca
    47941 aaaccgagac caacaccgag cgcggcaacc agccactgca atttcgacca ctcaatacca
    48001 atcattgaga agccgaccag cccgccaatc agcatcagca gatatttggt gatggtggtg
    48061 atggcgtaac ccgtacccgg cgttaaatcc aggtgctgca aaatcgccag ttccagcagc
    48121 gcgggcaggt tgcgcaccag ctgcgtggtg atgataaaca ccagaatggc aatcagcacc
    48181 gcaccgaggg taattggctc cagactttct acgccctgta ccgtggaggt gacatcccac
    48241 agcgaaatat tttcgaggaa gccgaaagcg gaatggattt ctgaccacag cacaatgacc
    48301 gaaagcaggg cgatcaacat caaaattgag cgcaccagcc gcaaggattg cgcactgatg
    48361 gcatcgagat cgacttcgct ttcatcgact tcaattgccc cttccgggct actgtgatga
    48421 tgtgcttcct cttcgccacg cgcacgttgc gctaacatct ctgcccggcg atgcttcgcc
    48481 cggtcaaacg ccagcctgcg gcgttggatc agcatccagc ggcggataac gtgataaacc
    48541 accagtagca ggaaccagat ggcaaccgag gtttcaagcc tcgctaacag cgcctgtgcc
    48601 gttgccagat aacccaccgc cgacgccaga atggcaacca gtggcgcgcc aatcatcatg
    48661 ttccacagca tatggttggt aatgttgtcg ccgctgccct ctttgttgag atacagtggg
    48721 atcccggcct ttttcaggct gagggtgacc accgccagcg caccgcaaat gaggataaag
    48781 caaagccgtc ccagcgaacc ggagaattca cggtcgtcga ggttatcgaa catcatcagc
    48841 gccataatca gcggcacaat aagcccgatg ctcatcaggt agtagcgcat cccacgggaa
    48901 acacgttcgc gcggccagcc aaaatgagcg ataaacaagc cgttcgggcg ggcaaaggtg
    48961 gcgcaaatca tcactaccca cagcagcggc acggtggccg ttacaccatc gccaatcgcg
    49021 accgccagcg gataaggcca cgcctcgcgc aagccgtaac ccagcgtcat ccacagcacc
    49081 ggtaacggtg acgcgacgag aatcgaccag aaaagagtgc gcaacgtcag ccagaagtga
    49141 tcctgagtca ctttgccaac tttcgccgcc gaacgttcaa gaaaacgggt gaaatagcgg
    49201 cgcgagtaaa tactgcaacc gaccagaatc aacgcgccaa acagcggcaa aattgtctct
    49261 ttgctggtca gcatcatcac actggctttg cccaactgac tgaaggtgtc cagcgaaatg
    49321 agacgacgca gatcctgggc gatttccagc ggccaggcga tggtcatcgg gcgcacgtca
    49381 gaggtccaga acagatagcg gtgcgttgct tcgttcacct ctttcagcgc atcctccagt
    49441 tgcccgttgg agactttcag cttggtcagt tccaatagta gcgtgtcgcc accctgcaac
    49501 aatgagttca gcaactcacg ctgagtgcgc agttgtgctt ccagaatacg gttttgctcg
    49561 gcggtcagcg gctgaccgtc ggcctgatgg atttgccgta gcagcggctg tttattaagc
    49621 agatcctcat aacgtaaccg ttgcacacgc aactgcgcca tttcggtatc aagctgttgt
    49681 ggttttggca tttctggcag ccgtgccacc tgcgcccgca gcgcttcgcc gagcagattg
    49741 gacgatccca gccattgcga ctgttcacgc agcgtattca acgcctgccg gacctgtaac
    49801 gtctggctgg cagcctgacg ctgttgcgag gcaacgagat ccatccgctg cgcctgttga
    49861 ttcaaagccg ccgatagttc gcggttaatt ttgaattgcg cgacgatatc tttcggcaaa
    49921 tcggcactgt tttctgccag taattcggta ctttccagcg cccgctccgc ctcaagctga
    49981 cgttggctgt ttaattgatt acgcaaggcc tgcaaatacg catccagttg ctggctctct
    50041 ttttccgcca gctctgagcg taagcgcgct aattcctggc ggttattggc agacagctgc
    50101 gccagctcca gttcatcaac gagcgcctta agacgtgcag agtcagactg caacgcgaaa
    50161 ttttgtgcct gattgagcgg agtattgcca gtaagcgtcc ccaggcggcg ctcgatctca
    50221 tttaactgac ggcgggcgtc ggtttgctgt tgcggcagtt gattcagcga atcggcaatc
    50281 tcgcgggcgc gctcctgctc ttgctgggcc tgacggcttt tatccagcaa ctggctgctg
    50341 acctggagaa tttcctgatt cagcgcgtcg gtagacattc ccggcgacac gctgcgcggc
    50401 tcgtcacgca tgttgtttaa ttgtgcgcgc agagtagcgg agagtttcgg ataattatcg
    50461 ataacttgct gatattgttt gatgcgctca agggaacctt ttcgttcctc aagcgcattt
    50521 aaggcagact ggagcgcctc tacgacttcc ggctgtgcgg gtttcgccgc ttttgcctgc
    50581 tccagttcct gagtgatttg tttgctatcg ggggccgtcg cggcgtacgc cccccaactg
    50641 aggcaccagg ccatcagaaa agtgataatc aggcgcacgt cagcgtttcc tttgatggat
    50701 tagacctggt cttttttgtc gtcaaccaat gggctggcgt cgtgttctgc ttcgatctct
    50761 tcagcaggaa gcggggcagg ttcagcgtct ggcgtaacaa aggtttcggt agatactgcc
    50821 agcggctggc caattttcgt gacagacagg ctttccagtt gctcaaccag attcacttta
    50881 cccggtgcaa acaggttgat aacggtggaa ccgagtttaa agcgacccat ttcctggcct
    50941 ttcagcagtg ccacagaacc gtcgttttcc ccggcaggcc aggtccagcg cttgatgata
    51001 ccttcgcgcg gcggcgtaat ggtgcccgcc cagaccgtct caatgctgcc aacaatcgtc
    51061 gctccgacca gaatctgcgc cattgggcca aattcggtat cgaaaaggca aatcacgcgt
    51121 tcgttacggg caaacagatt cggcacgttc tgagcggtga gatggttaac ggagaagaga
    51181 tcgcccggca cgtagatcat ctcacgcaga ataccgttgc acggcatgtg tacgcggtgg
    51241 tagtcacgcg gggagaggta agtggtcaca aacgtaccgt tgcggaacag gtccgccatc
    51301 agatagttgc ctgccagcaa ggcttcgagg ctgtagttgt ggcctttggc ttgcaggatt
    51361 ttatcttctt cgattttacc caactggctg ataacgccat cggcaggcat gaccaggaca
    51421 ttcggatcgg tatcgattgg gcgtacttcg tcacgcagcg gacggacaaa gaattcgtta
    51481 aaggtgcggt agctggcggt gtccggcttt tgcgcctctt tcatgtcgac cttgtagtat
    51541 ttaacgaaca gatcgataac cagttttgtc agccatcctg cccgcttgct tgcgccccaa
    51601 cccgccaggc gagtaagcca tagtttcggc agaatgtact gtagcgaaag tttaaatgaa
    51661 tttaacaagg tagcctccag gccattgttt tgtcgttcct gatccggcct acatgccgga
    51721 tcctgaaaaa aaggggacga ttctaacgac ggttagctta attgtcagtc atccgtatca
    51781 gaaaagtttt tacgcgtttt tacctgcgcc atgctttcca gaatacggtg atagttttcg
    51841 aaacgggttt ccgcgatttt cccttcctca accgcttccc ggatagcgca gcccggatcg
    51901 gtatcgtgtt tgcaatcgcg atatttacac agacctaagt agtcatggaa ttcgacaaag
    51961 ccctgagtga tttgttccgg ctccaggtgc cagaggccga actcacgcac accaggggaa
    52021 tcaatcacat caccgccgtg cgggaagtga tacagccgag cggcggtcgt ggtgtgctga
    52081 ccgagccccg agttgtcaga gacatcgttg gtcaggatct ctttttgcag ccccagcaac
    52141 gcgttcagca ggctggattt gccgacgcca gactgcccgg caaaaatgct gatgcgcccg
    52201 gtcaacgcct cttccagcgg ttttagccca tcctgagtat ggctggaaac catcaatacg
    52261 cgataaccga tattgcggta gatatccatc tgctcgttga cgaacgccat gccttcgtcg
    52321 tccagcaggt ctatcttgtt gagcacaata atcggctcaa tctgcaaggt ttcgcaggcc
    52381 accaggtaac ggtcgataat attgagcgac agctccggca aaatggcgga gacaatgaca
    52441 atctggtcga tgttggcggc aataggtttc acgccgtcgt agaaatccgg acgcgtcaac
    52501 accgaggtgc gctcatgcac cgcttccacg atccctttga catttacgcc ttccgccgcc
    52561 ggtttacctg gacgccagac tacgcggtcg ccggtcacca gcgaacggat ggtacggcga
    52621 atattgcagc ggtgaacgtc gccatcggcg gattccacat cagcgtgcat accaaagcgg
    52681 ctgatgacga taccttcatc aggctcgcca aacagattgt cgtcgtagtc gggcttctcc
    52741 ttagacgttt taagacgacg ctggtgattg gcgttcacgc ggcgctgctg gcctttggag
    52801 agtttatttt tactcaatcg tactgactcc tggtcgcccc tgatgggcaa aacatctatg
    52861 atacacgcaa ttgtggatca atatagtcac tgtgaatggg tggaaaatag catgagtgcc
    52921 aatgaaaaca acctgatttg gatcgatctt gagatgaccg gtctggatcc cgagcgcgat
    52981 cgcattattg agattgccac gctggtgacc gatgccaatc tgaatattct ggcagaaggg
    53041 ccgaccattg cagtacatca gtccgatgaa cagctggcgc tgatggatga ctggaacgtg
    53101 cgcacccata ccgccagcgg gctggtagag cgcgtgaaag cgagcacgat gggcgatcgg
    53161 gaagctgaac tggcaacgct cgaattctta aaacagtggg tgcctgcggg aaaatcgccg
    53221 atttgcggta acagcatcgg acaggaccgt cgtttcctgt ttaaatacat gccggagctg
    53281 gaagcctact tccactaccg ttatctcgat gtcagcaccc tgaaagagct ggcgcgccgc
    53341 tggaagccgg aaattctgga tggttttacc aagcagggga cgcatcaggc gatggatgat
    53401 atccgtgaat cggtggcgga gctggcttac taccgcgagc attttatcaa gctgtaaaat
    53461 ttttcctggt cacgtaagcg ccggaatcca cttgcggcgg cgtgattttg acgctaaatt
    53521 gaacgtcttg ctgataattt cagcacttga gataaaaacg caaaaaaaac ttttttgggg
    53581 ggttgcagag ggaaagattt ctcgtataat gcgcctcccg taacgacgca gaaatgcgaa
    53641 aattacgaaa gcaaaattaa gtagtacgcg ggaatagctc agttggtaga gcacgacctt
    53701 gccaaggtcg gggtcgcgag ttcgagtctc gtttcccgct ccaaaatttg aaaagtgctg
    53761 caaagcacag accacccaat cgcagtattt cgaattgcga caaggcggca actgaacgaa
    53821 tccttg
//
LOCUS       DAOFQD010000018        51718 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.018, whole genome shotgun sequence.
ACCESSION   DAOFQD010000018 DAOFQD010000000
VERSION     DAOFQD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.018"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(180..1034)
                     /gene="kdsA"
                     /locus_tag="QBF46_001121"
     CDS             complement(180..1034)
                     /gene="kdsA"
                     /locus_tag="QBF46_001121"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDQ6673332.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(1070..1879)
                     /locus_tag="QBF46_001122"
     CDS             complement(1070..1879)
                     /locus_tag="QBF46_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDQ6673333.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(1883..2275)
                     /locus_tag="QBF46_001123"
     CDS             complement(1883..2275)
                     /locus_tag="QBF46_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDQ6673334.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSSIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIFAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(2272..3105)
                     /gene="prmC"
                     /locus_tag="QBF46_001124"
     CDS             complement(2272..3105)
                     /gene="prmC"
                     /locus_tag="QBF46_001124"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDQ6673335.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDPVALAQRNAQHLAIKN
                     IRILQSDWFSALAGQQFTMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(3105..4187)
                     /gene="prfA"
                     /locus_tag="QBF46_001125"
     CDS             complement(3105..4187)
                     /gene="prfA"
                     /locus_tag="QBF46_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDQ6673336.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(4229..5485)
                     /gene="hemA"
                     /locus_tag="QBF46_001126"
     CDS             complement(4229..5485)
                     /gene="hemA"
                     /locus_tag="QBF46_001126"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDQ6673337.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            5699..6322
                     /gene="lolB"
                     /locus_tag="QBF46_001127"
     CDS             5699..6322
                     /gene="lolB"
                     /locus_tag="QBF46_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDQ6673338.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            6322..7173
                     /gene="ispE"
                     /locus_tag="QBF46_001128"
     CDS             6322..7173
                     /gene="ispE"
                     /locus_tag="QBF46_001128"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDQ6673339.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            7324..8271
                     /gene="prs"
                     /locus_tag="QBF46_001129"
     CDS             7324..8271
                     /gene="prs"
                     /locus_tag="QBF46_001129"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDQ6673340.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            8396..10075
                     /gene="dauA"
                     /locus_tag="QBF46_001130"
     CDS             8396..10075
                     /gene="dauA"
                     /locus_tag="QBF46_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDQ6673341.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(10130..10408)
                     /gene="ychH"
                     /locus_tag="QBF46_001131"
     CDS             complement(10130..10408)
                     /gene="ychH"
                     /locus_tag="QBF46_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDQ6673342.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVDGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            10686..11270
                     /gene="pth"
                     /locus_tag="QBF46_001132"
     CDS             10686..11270
                     /gene="pth"
                     /locus_tag="QBF46_001132"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDQ6673343.1"
                     /translation="MTIKLIIGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            11387..12478
                     /gene="ychF"
                     /locus_tag="QBF46_001133"
     CDS             11387..12478
                     /gene="ychF"
                     /locus_tag="QBF46_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDQ6673344.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            13247..16114
                     /locus_tag="QBF46_001134"
     CDS             13247..16114
                     /locus_tag="QBF46_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6673345.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDAIINFTGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTINSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMTMKVSNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(16214..18133)
                     /gene="dhaR"
                     /locus_tag="QBF46_001135"
     CDS             complement(16214..18133)
                     /gene="dhaR"
                     /locus_tag="QBF46_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDQ6673346.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            18361..19431
                     /gene="dhaK"
                     /locus_tag="QBF46_001136"
     CDS             18361..19431
                     /gene="dhaK"
                     /locus_tag="QBF46_001136"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDQ6673347.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            19442..20074
                     /gene="dhaL"
                     /locus_tag="QBF46_001137"
     CDS             19442..20074
                     /gene="dhaL"
                     /locus_tag="QBF46_001137"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDQ6673348.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            20085..21503
                     /gene="dhaM"
                     /locus_tag="QBF46_001138"
     CDS             20085..21503
                     /gene="dhaM"
                     /locus_tag="QBF46_001138"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDQ6673349.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISASCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNVDMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            21823..23520
                     /gene="treA"
                     /locus_tag="QBF46_001139"
     CDS             21823..23520
                     /gene="treA"
                     /locus_tag="QBF46_001139"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDQ6673350.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLEEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(23599..24039)
                     /locus_tag="QBF46_001140"
     CDS             complement(23599..24039)
                     /locus_tag="QBF46_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673351.1"
                     /translation="MIQKINILDSSQSGVKNEADSEAVLAALIEHLELINPSGRFSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(24214..24468)
                     /locus_tag="QBF46_001141"
     CDS             complement(24214..24468)
                     /locus_tag="QBF46_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDQ6673352.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            24669..25403
                     /locus_tag="QBF46_001142"
     CDS             24669..25403
                     /locus_tag="QBF46_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDQ6673353.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYELSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(25405..26016)
                     /gene="emtA"
                     /locus_tag="QBF46_001143"
     CDS             complement(25405..26016)
                     /gene="emtA"
                     /locus_tag="QBF46_001143"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDQ6673354.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            26116..27030
                     /gene="ldcA"
                     /locus_tag="QBF46_001144"
     CDS             26116..27030
                     /gene="ldcA"
                     /locus_tag="QBF46_001144"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDQ6673355.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            27125..28861
                     /locus_tag="QBF46_001145"
     CDS             27125..28861
                     /locus_tag="QBF46_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDQ6673356.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(29247..30317)
                     /gene="dadX"
                     /locus_tag="QBF46_001146"
     CDS             complement(29247..30317)
                     /gene="dadX"
                     /locus_tag="QBF46_001146"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDQ6673357.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(30327..31625)
                     /gene="dadA"
                     /locus_tag="QBF46_001147"
     CDS             complement(30327..31625)
                     /gene="dadA"
                     /locus_tag="QBF46_001147"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDQ6673358.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            31955..33487
                     /locus_tag="QBF46_001148"
     CDS             31955..33487
                     /locus_tag="QBF46_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDQ6673359.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(33539..34258)
                     /gene="fadR"
                     /locus_tag="QBF46_001149"
     CDS             complement(33539..34258)
                     /gene="fadR"
                     /locus_tag="QBF46_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDQ6673360.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            34480..36021
                     /gene="nhaB"
                     /locus_tag="QBF46_001150"
     CDS             34480..36021
                     /gene="nhaB"
                     /locus_tag="QBF46_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDQ6673361.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            36167..36697
                     /gene="dsbB"
                     /locus_tag="QBF46_001151"
     CDS             36167..36697
                     /gene="dsbB"
                     /locus_tag="QBF46_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDQ6673362.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(36743..38011)
                     /gene="umuC"
                     /locus_tag="QBF46_001152"
     CDS             complement(36743..38011)
                     /gene="umuC"
                     /locus_tag="QBF46_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDQ6673363.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWHRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(38011..38430)
                     /locus_tag="QBF46_001153"
     CDS             complement(38011..38430)
                     /locus_tag="QBF46_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDQ6673364.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            38627..39016
                     /locus_tag="QBF46_001154"
     CDS             38627..39016
                     /locus_tag="QBF46_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HDQ6673365.1"
                     /translation="MEAFAQKGILIKVLDDGIKKLCSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKIINTCNEYQKRHGKKTLFEVSEV"
     gene            complement(39222..39668)
                     /locus_tag="QBF46_001155"
     CDS             complement(39222..39668)
                     /locus_tag="QBF46_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDQ6673366.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(39760..40419)
                     /locus_tag="QBF46_001156"
     CDS             complement(39760..40419)
                     /locus_tag="QBF46_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDQ6673367.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(40491..40784)
                     /locus_tag="QBF46_001157"
     CDS             complement(40491..40784)
                     /locus_tag="QBF46_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDQ6673368.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            41026..41427
                     /locus_tag="QBF46_001158"
     CDS             41026..41427
                     /locus_tag="QBF46_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDQ6673369.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(41547..41915)
                     /locus_tag="QBF46_001159"
     CDS             complement(41547..41915)
                     /locus_tag="QBF46_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HDQ6673370.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            42435..43130
                     /gene="minC"
                     /locus_tag="QBF46_001160"
     CDS             42435..43130
                     /gene="minC"
                     /locus_tag="QBF46_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDQ6673371.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            43154..43966
                     /gene="minD"
                     /locus_tag="QBF46_001161"
     CDS             43154..43966
                     /gene="minD"
                     /locus_tag="QBF46_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDQ6673372.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            43970..44236
                     /gene="minE"
                     /locus_tag="QBF46_001162"
     CDS             43970..44236
                     /gene="minE"
                     /locus_tag="QBF46_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDQ6673373.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(44608..44951)
                     /locus_tag="QBF46_001163"
                     /pseudo
     CDS             complement(44608..44951)
                     /locus_tag="QBF46_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(44986..45105)
                     /locus_tag="QBF46_001164"
     CDS             complement(44986..45105)
                     /locus_tag="QBF46_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDQ6673374.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(45066..45251)
                     /locus_tag="QBF46_001165"
     CDS             complement(45066..45251)
                     /locus_tag="QBF46_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673375.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYQKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            45352..45525
                     /locus_tag="QBF46_001166"
     CDS             45352..45525
                     /locus_tag="QBF46_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673376.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            45527..45871
                     /locus_tag="QBF46_001167"
     CDS             45527..45871
                     /locus_tag="QBF46_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDQ6673377.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            45875..46210
                     /locus_tag="QBF46_001168"
     CDS             45875..46210
                     /locus_tag="QBF46_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDQ6673378.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(46267..48915)
                     /locus_tag="QBF46_001169"
     CDS             complement(46267..48915)
                     /locus_tag="QBF46_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6673379.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYATQLLDDYSIISYMTDEESPIE
                     IKDNNPISNGEYLTTEDESHAVKVDDGVTGYINNASVMTSGDGSYGISVDSQNKVLYI
                     SDSDIKTSGSVSDKENGGITASAVVSEFGGTIFMNGDNSVESGGAYSAGLLSQVNDSE
                     KMVNNTRLETTDKTNIVTSGENAVGVLACSSPGESRTCVDAVDDEVSDSNSYEVISRA
                     DLKMNGGSITTNGINSYGAYANGKKAYINLDYVALETVADGSYAVAIRQGNIDIKNSS
                     ITTTGTKAPIAKIYNGGELFFSNVTAVSKQDKGISIDASNIDSQAKIALLSVELSSAL
                     DSIDVNKTTTDVSFLNRSIITPGNNVLVNNTGGDLNIISSDSILNGATKLVSGTTTLK
                     LSENTIWNMKDDSVVTHLTNSDSIINLSYDDGQTFTQGKTLTVKGNYVGNNGQLNIRT
                     VLGDDKSATDRLIVEGNTSGSTTVYVKNAGGSGAATLNGIELITVNGDESPADAFRQG
                     DARIAAGAFEYQLKQQGKNWYLTSYQSVNEEDNSSEGNSESTETPTPVLRPEAGSYVA
                     NLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSH
                     RYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRGYSAGLYA
                     TWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSF
                     GLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEK
                     NSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGNLNPAASV
                     WGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(49315..49533)
                     /locus_tag="QBF46_001170"
     CDS             complement(49315..49533)
                     /locus_tag="QBF46_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673380.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(49665..51188)
                     /locus_tag="QBF46_001171"
     CDS             complement(49665..51188)
                     /locus_tag="QBF46_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDQ6673381.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESIRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(51520..>51718)
                     /locus_tag="QBF46_001172"
     CDS             complement(51520..>51718)
                     /locus_tag="QBF46_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673382.1"
                     /translation="NLEREVFMTHGYVDSHIIDQALRLRLKDETSVILSDLYLQILQY
                     IEMHKTTLTDIIINDRESVLS"
BASE COUNT        13174 a        12970 c        12425 g        13149 t
ORIGIN      
        1 gcgattgtcg gctggtggcg taaccggaag taaggtgaaa acgggcgtca ggctgccgtg
       61 atggcaatat gcgcccgaaa ccggccgcag gggtaacagc ctgcggcctt ttttgtatcc
      121 ggcacccata aaaaagtctt aacgcacaac gctaagactt tatttttcaa acaaaaagat
      181 tacttgctgg tatccagttc ttcgaaacct ttcaccagat catcaatcgc tttcatctgc
      241 ttgaggaacg gttccagttt agccagcggc agcgcggatg gaccatcaca tttcgcatgt
      301 tccggatccg gatgcgcttc aataaacagc cctgccagac ctaccgccat accggctcgt
      361 gccagctcag ccacctgagc acgacgaccg ccggaagctg cgccaaaagg atcgcggcat
      421 tgcagtgcgt gggtcacgtc gaaaatcacc ggcgagttac cagacacttt cttcataatg
      481 ctgaagccca gcatatcgac aaccaggttg tcatagccga agttagcacc gcgatcgcaa
      541 agaatcactt tttcgttgcc gccttctttg aatttatcaa cgatattacc catctgtccc
      601 gggctgacaa actgtggttt cttgacgtta attaccgcac cggttttcgc catggcttca
      661 accaggtcag tctggcgagc aagaaacgcc ggcaactgaa tcacatccac gacatcagca
      721 acgggctgtg cctgacttgg ttcgtgaacg tcggtgataa ttttcacgcc aaaagtctgc
      781 ttcaactcct ggaagatttt catcccttct tccaggcccg gtccacgata agagtggatg
      841 gaggagcggt tggctttgtc aaaagaggct ttgaacacgt aagggatacc cagtttctgg
      901 gtcacggtta cgtagtgctc gcaaatgcgc atcgccagat cgcgagattc cagcacgttc
      961 ataccgccaa acagtacgaa cggcaggtca tttgctacgt tgatgtcgcc aatgctaacc
     1021 actttttgtt tcataggatc gccttaatct tgagtaaaat gtcgattaat taatgcagca
     1081 caatatgttt atgcgcgatg ttatttattt gcgcacggat catttcgctg atcgggtctt
     1141 ccggacactg ttcaacgaaa taacttaaat cgttcaacgc aacgtgttcg caatccagtt
     1201 gcgcataaat caacccgcga tcgcgaattt catagggatc ttcagggttg aattgtaata
     1261 aagcttcgct ggtgcgtaac gccagctcca tctgattttc ttccatcaac gaggctttga
     1321 gtgtatccag caatttgcgg attacctcaa tgttatcagc ttcatcaagg tcttcataaa
     1381 acagttccgc cgacgggctg atatttccct ttaaccatac gtccagcata tgttcgctta
     1441 acgattcacc gttaaaagga ttaatcagcc aaatttcgcc atccggacat tcaatgcgca
     1501 atatcagctg cgtagggaaa atcaccggca gcagcggcaa atcgagacga ttcgcgaccc
     1561 ataataaaac cgcacctaat gataccgcac tgccctgtcg attctttaac acctggtcca
     1621 gccataatgc atcggaaaga cgataaacgc cgcgtgaggc tttaaatccc cagtcgccgt
     1681 aaaacagcgc gatcagtttt tccagctgct cttctaaagg cagaagctgg ctgatttctt
     1741 ccttcgccag actaacgaga cgctccagtt cgtcgtaaac atcttgcgag ggaaaatcgc
     1801 ggcggattgc ttcgcaagcc aggatcatgc cttcgcacaa tggcgcttta ttaaattcga
     1861 aatcagctaa cgatctcatg acttacccca gtaacggtac ttttgtggtg gcgagtttaa
     1921 tgatgatgta cagaaccacc aacgccagcg ggaaggcaaa aatgcgcgcc tgctgactgt
     1981 gcatacgacg ataatcgagt gcaataaaac ccaaaacgat ataaatgata actccaaaca
     2041 gcttttcagt cagccactgt gcctgcccgg aaaatggcag gatgtgcgct ttagccatca
     2101 acgcaatgct gcttaacaat aacaccgtat cgacaaccgg cggcacaatg cgcgtccagc
     2161 gagcaaatgc ctgtggatgc ttctgataac gtagccagaa acgtaaggtt aatagcccaa
     2221 cagaaagcgc gatactaata agatgaacac taagcagtgt agaaaaactt gtcattgata
     2281 atagcggccg agcgttacgc gctcgttatc accatagtca cggcaggttt cgacgtcatg
     2341 ataccccgcg aggataaatg cttgtcgcac cgcttcgccc tgctgccagc catgttccag
     2401 aagcagaaag ccgccggata ccagcgcgtt acgcgactgt tcgatgatat gcacgatgtc
     2461 tgccattcca ctgtctgccg caaccagcgc agtgagcggc tcaaagcgga catcgccttg
     2521 ttgaagatgt gggtcctgct cgtcaatata cggcggattg ctgacaatca tcgtaaactg
     2581 ctgcccggcc agcgcactaa accagtcgct ttgcagaatg cggatatttt tgatcgccag
     2641 atgctgggca ttacgttgtg ccagagcgac aggatcgggc atacgatcta cagcggtaat
     2701 ttcgcagtcc gggcgctcgc tagccagcgc aagcgcaatc gccccggtac ccgtcccgag
     2761 atcgagaata cggcaaggtt gttcaggcaa ccgcgccagt gcctgctcca ccagacactc
     2821 cgtatccggg cgcggaatta aggtcgctgg cgaaacaaat aacggcaacg accagaattc
     2881 tcgcaccccg gttaaatgag caatgggttc accatcgcga cgacgtgtca gtagcgcatc
     2941 aagttgctga cattgttcgt cagtcagctg cgtttcacca aaggcgagga taaaagtacg
     3001 ccctttgccg gtaacatgtt ccagcaggat ttcagcatca cgccgcgggc tttcgctcgc
     3061 ctgaagttgg cttattgctt cacgtaacca gtgttgatat tccattattc ctgctcggac
     3121 aacgccgcca gttggtcggc ctgatgttcc tggataatcg gttcaatcag catatccagc
     3181 ttaccttcca tcacttcatc caggcggtag agcgtcaggt tgatgcggtg atcggtaacg
     3241 cgcccctgtg ggaagttgta agtacggtta cggtcgctgc gatcgccact ccccagcagg
     3301 ttacgacggg tagacgcttc ggcctgttga cgttttgcca tttcagcagc gtggatgcga
     3361 gcaccgagta cagaaagtgc tttagctttg tttttatgtt gtgaacgttc gtcctgacat
     3421 tcaacaacaa tcccggtcgg caagtgagta atacgaattg ccgaatcggt ggtgttaacg
     3481 tgctgaccac ccgcccctga cgagcggaaa gtatcaatgc gtaaatctgc tgggttgatg
     3541 tccggcagtt ctgcgtcagg cagctctggc attaccgcaa cggtacaagc agaagtatga
     3601 atacgcccct gcgattccgt agcaggaaca cgctgcacgc gatgaccgcc agattcaaat
     3661 ttcagacgac catacacacc atcaccgcta attttggcga tgatctcttt ataaccacca
     3721 tgttcaccct cgctggcgct catgatttct acccgccagc ggcgggcttc ggcataacgg
     3781 ctgtacatac ggaacagatc gcctgcgaac agcgccgctt cgtcgccgcc ggttccggca
     3841 cggacttcga ggaaggcgtt acgttcgtca tcaggatctt ttggtagtaa cagaacctgt
     3901 aattgctgtt ccagttgctc gcttttttct ttagcttcgc gcagttcatc ctgcgccatc
     3961 tcacgcattt caggatcatc gagcatcatc tgtgcggttt cgatatcttc ctgaacctgt
     4021 tgccagtcgg taaaacagcg cgaaacatca cttaactgcg catattcgcg tgataatgcg
     4081 cgaaaacgtt cctggtcggc gatagtttgc gcgtcaccca gcaacgcctg aacttcttca
     4141 tggcgttcat gcagggcttc cagtttggca acgatagaag gcttcatagg cgtaaatgca
     4201 ccctgtaaaa aaagaaaatg atgtactgct actccagccc gaggctgtcg cgcagaatat
     4261 tcaggcgttc gttatccccg tcacgggcgg cctgttgaag tgatttcgtt ggcgcatgga
     4321 tcaagcggtt agtcagtttc catgccagat cctgcataat ggcttgcgcg tcgccgccct
     4381 gctcaagggc cgctaacgct ttggcggtta actcatcgcg aacttgctct gcctggctgc
     4441 gatactcgcg aatggtttcg ctggcgcttt gtgctcgcag ccacgccata aattcgctgg
     4501 tttcctgagc gacaatagtt tccgcctcaa ccgctgcggc tttacgctgc gccaggttgt
     4561 gcgaaatgat gctttgcaga tcgtccacgc tataaagata agcattcgcc agtttgccaa
     4621 cttccggctc aacatcgcgc ggaacggcaa tatccaccaa cagcattggt tggttgcgac
     4681 ggctttttaa tgcgcgctcc accatgcctt tcccgataat cggtaacggg ctggcggtgg
     4741 aactgatgat gatatcggct tcgcgcagac gttcgtcgat ctcactcagg gcaatcactt
     4801 ccgcgccaac ttcatctgcc agtatttggg cacgttcgcg agtgcggttg gcgataatca
     4861 tcttctgtac tttatgttcg cgcagatgac gcgctaccag ctcgatggtt tcgcccgcgc
     4921 ctaccagcaa cactgtgacc gtagagagcg attcaaagat ctgccgcgcc agcgtacaag
     4981 ccgcaaaagc gacagacaca gcgctggcac cgatatctgt ttcagtgcga acgcgtttcg
     5041 ctacagagaa agatttctgg aacatgcgtt ccagttcgct ggccttcata tggccttttt
     5101 gcgaatcggc aaacgctttt ttaacctgac cgaggatctg cggctcccca agaaccaatg
     5161 aatccaggcc gctggcaaca cgcattaaat ggctaaccgc gtcgttatcc tgatgccagt
     5221 agaggctttt acgcagatct tcttcattaa gattgtgata atcgcaaagc cagcggatta
     5281 acgcctcttg caggttatcc tgctcttcaa cgctaagata aagttccgtg cggttgcacg
     5341 tcgacagcac cacgccgccc tgcaccatcg gctgcgcaag caggctgtca agcgcctgat
     5401 cgagcttatc cggcgaaaac gatacacgtt ctcgcagcga tacaggtgcc gttttatggt
     5461 tgataccgag tgctaaaagg gtcatgtctg cgggaaataa taccaacgtt gatagggtta
     5521 gtctgcttgc atcatacagg atgcgtaaga tcaataaaag agagcgcccc cttttggagt
     5581 aattgccgaa agccgttaga ttctggcaat taagacaact tgaacataga cgatagcgga
     5641 cggtaacgct agcattaagg gttataactg caacgtatct caaggacttg tcatcactat
     5701 gcccctgccc gattttcgtc ttatccgcct gctaccgctg gctgctcttg tgctcactgc
     5761 ctgttccgtt accacgccca aaggtcctgg caaaagcccg gattcgccac aatggcgtca
     5821 gcatcagcaa gacgtgcgca atcttaatca gtatcagact cgcggcgcgt tcgcttatat
     5881 ttctgaccaa caaaaagtgt acgcccgctt tttctggcag caaaccggcc aggatcgcta
     5941 ccgtctgctg ctcactaacc cattgggcag cacggaactg gagctgaatg ctcaaccggg
     6001 taacgtgcag ttagtcgaca ataaaggtca gcgttatacc gccgatgacg ccgaagagat
     6061 gattggcaaa ttgaccggaa tgccaattcc gctcaacagc ttgcgccagt ggattttagg
     6121 tttaccgggt gatgcaaccg actacaaact ggacgaccag taccgcctga gcgaaattac
     6181 ctacagccag aatggcaaaa actggaaggt tgtttatggt ggttatgaca ccaaaacgca
     6241 acctgcgatg ccagccaata tggaactcac cgacggtggt caacgcatca agttaaaaat
     6301 ggataactgg atagtgaaat aatgcggaca cagtggccct ctccggcaaa acttaatctg
     6361 tttttataca ttaccggtca gcgtgcggat ggttaccaca cgctgcaaac gctgtttcag
     6421 tttcttgatt acggcgacac catcagcatt gagcttcgtg acgatgggga tattcgtctg
     6481 ttaacgcccg ttgaaggcgt ggaacatgaa gataacctga tcgttcgcgc agcgcggttg
     6541 ttgatgaaaa ctgcggcaga cagcgggcgt cttccgacgg gaagcggtgc ggatatcagc
     6601 attgataagc gtttgccgat gggcggcggt ctcggcggtg gttcatccaa tgccgcgacg
     6661 gtcctggtgg cattaaatca tctctggcaa tgcgggctaa gcatggatga gctggcggaa
     6721 atggggctga cgctgggcgc agatgttcct gtctttgttc gggggcatgc cgcgtttgcc
     6781 gaaggcgttg gtgaaatact aacgccggtg gatccgccag agaagtggta tctggtggcg
     6841 caccctggtg taagtattcc gactccggtg atttttaaag atcctgaact cccgcgcaat
     6901 acgccaaaaa ggtcaataga aacgttgcta aaatgtgaat tcagcaatga ttgcgaggtt
     6961 atcgcaagaa aacgttttcg cgaggttgat gcggtgcttt cctggctgtt agaatacgcc
     7021 ccgtcgcgcc tgactgggac aggggcctgt gtctttgctg aatttgatac agagtctgaa
     7081 gcccgccagg tgctagagca agccccggaa tggctcaatg gctttgtggc gaaaggcgtt
     7141 aatctttccc cattgcacag agccatgctt taagccgggc aagctgagtt tcggtgacaa
     7201 cgtcaccttg ttccagacgt tgcatcgcgc tctttaatac accgcctgga aaggatcatg
     7261 cctggcccgc acagttttcg gcagattctt tccaccaatg gacgcatgcc tgaggttctt
     7321 ctcgtgcctg atatgaagct ttttgctggt aacgccaccc cggaactagc acaacgtatt
     7381 gccaaccgcc tgtacacttc actcggcgac gccgctgtag gtcgctttag cgatggcgaa
     7441 gtcagcgtac aaattaatga aaatgtacgc ggtggtgata ttttcatcat ccagtccact
     7501 tgtgccccta ctaacgacaa cctgatggaa ttagtcgtta tggttgatgc cctgcgtcgt
     7561 gcttccgcag gtcgtatcac cgctgttatc ccctactttg gctatgcgcg ccaggaccgt
     7621 cgcgtccgtt ccgctcgtgt accaatcact gcgaaagtgg ttgcagactt cctctccagc
     7681 gtcggtgttg accgtgtgct gacagtggat ctgcacgctg aacagattca gggtttcttc
     7741 gacgttccgg ttgataacgt atttggtagc ccgatcctgc tggaagacat gctgcagctg
     7801 aatctggata acccaattgt ggtttctccg gacatcggcg gcgttgtgcg tgcccgcgct
     7861 atcgctaagc tgctgaacga taccgatatg gcaatcatcg acaaacgtcg tccgcgtgcg
     7921 aacgtttcac aggtgatgca tatcatcggt gacgttgcag gtcgtgactg cgtactggtc
     7981 gatgatatga tcgacactgg cggtacgctg tgtaaagctg ctgaagctct gaaagaacgt
     8041 ggtgctaaac gtgtatttgc gtacgcgact cacccgatct tctctggcaa cgcggcgaac
     8101 aacctgcgta actctgtaat tgatgaagtc gttgtctgcg ataccattcc gctgagcgat
     8161 gaaatcaaat cactgccgaa cgtgcgtact ctgaccctgt caggtatgct ggccgaagcg
     8221 attcgtcgta tcagcaacga agaatcgatc tctgccatgt tcgaacacta atcgaacccg
     8281 gctcaaagac ccgctgcggc gggttttttt gtctgtaata tccatttgta tgacctatgc
     8341 ctccttcacc tgccatttag ttgacagatg atgcgctcac ggatgaaaca ttattgtgaa
     8401 caaaatattt tcctcacatg tgatgccttt ccgcgctctg atcgacgctt gctggaaaga
     8461 aaaatatact gccgcacggt ttacccgtga cctgattgcc gggataaccg tcgggattat
     8521 tgctatcccg ctggcgatgg cgttggctat tggtagtggt gtggcacccc agtacggttt
     8581 atataccgca gctgttgcgg ggattgtcat tgctctgacg ggtgggtcac gctttagcgt
     8641 ttccggtccg actgcggcat ttgtggtaat tctctatccc gtgtcgcaac agtttggact
     8701 ggcaggactg ctggttgcga ccttgctgtc ggggatcttt ttgattctga tgggtctggc
     8761 acgctttggt cgcctgattg agtatattcc ggtttccgtc accttaggtt tcacctcggg
     8821 tatcgggatc accatcggta ccatgcagat taaagatttt ctcggtctgc aaatggccca
     8881 tgtcccggaa cattatctac aaaaagtcgg cgcattattt atggcgctgc cgaccattaa
     8941 tgtgggtgat gctgccattg gcattgtgac gctaggtatt cttgtttttt ggccgcgtct
     9001 gggcattcgt ttacccggtc accttccggc cttgctggct ggttgcgcgg tgatggggat
     9061 tgttaacctg ctcggcggac atgttgctac catcggttcg caattccact acgtcctggc
     9121 cgatggttct cagggtaacg gtattccgca actgctgccg caactggtgc tgccgtggga
     9181 tctgcctaat tcagaattca cgctaacctg ggattctatt cgcacactgc tgcctgcggc
     9241 attctcaatg gcaatgctcg gcgcaatcga atctctgctc tgcgccgtgg tactggatgg
     9301 tatgaccggg acgaaacaca aggcgaacag cgaactggtt ggacagggac tggggaatat
     9361 tatcgctccg ttctttggtg gtattaccgc tacagctgcc atcgcgcgtt ctgccgctaa
     9421 cgtccgtgcc ggggcaactt cccctatctc ggcggtgatc cactctattc tggttattct
     9481 tgccctgctg gtactggcac cgctgctctc ctggctgccg ctttccgcta tggcagccct
     9541 gctgttgatg gtggcgtgga acatgagtga agcgcataaa gtggtcgact tgctgcgtca
     9601 tgcaccgaaa gatgacatca ttgtcatgct gctgtgcatg tcgctgaccg tgctgtttga
     9661 tatggttatt gccatcagcg tggggatcgt gctggcatcg ctgctgttta tgcgtcgtat
     9721 cgcacgtatg actcgcctgg caccggtagt cgtagatgtt ccagacgatg ttctggtact
     9781 gcgcgttatt ggcccgctgt tttttgctgc tgctgaaggc ttgttcacgg acctggagtc
     9841 acgtcttgaa ggcaaacgga ttgtgattct gaagtgggat gccgttccgg tacttgatgc
     9901 tggtggtctt gatgcgttcc agcgttttgt gaagcgtctg cccgaaggat gtgaactgcg
     9961 cgtgtgcaac gtggaattcc agccactgcg cactatggct cgcgcaggca ttcaaccgat
    10021 cccgggacgc ctcgcgttct tcccgaatcg tcgcgcggcg atggcggatt tataagtacg
    10081 agattgacca gtcagcgcaa actgactggt cagcaaactg cattatttgt tagctatgac
    10141 ggcgattatc gctacggcgg caacgtttgt catagtgacg aacccaccag taacggtcgc
    10201 acacctgttc atgcccgtca acacgggcac ccgccagcca gagaatggca ccgacgaaaa
    10261 tacttagcac tgcaccatgt gcgaaatact ggggcatatt aaactgtggt aactggttga
    10321 ggattgaata ccccacgccg accaccatta ccaccagacc caaccccatg agcacgttac
    10381 cgagtaacga agcgtttttg cgtttcatat gtcacctccg gaactttctg ggttgtaaca
    10441 gggaataccc ctcttcctta tgtgtaaagt atagacaaca cccagtgggt tatgtgcggg
    10501 cgtgatcaca attacaaccc ttatttcaac aaaactttac aaataaacgc ctgaacacct
    10561 tgactttcta atagtccact caggcaatta ttacgttatc tgcatttgat acgcagtttt
    10621 ttgtcaagcg gggccgcaac cagtaaacta cgcgccagtt atgtacacac tcaggacaaa
    10681 aaaacgtgac gattaaattg attatcggcc tggcgaaccc cggtgctgaa tacgccgcaa
    10741 cgcgacataa tgctggtgcc tggttcgttg acttactggc agagcgtttg cgcgctccgc
    10801 tgcgcgaaga ggctaaattc tttggttata cttcgcgagt cactcttgga ggcgaagatg
    10861 tccgcctgtt agtcccgact acatttatga atctcagcgg caaagccgtt gcggcgatgg
    10921 ccagtttttt ccgcattaat ccggacgaaa ttctggtggc ccacgacgaa ctggatctgc
    10981 ctcctggcgt cgccaaattt aaattgggcg gtggccatgg tggtcacaat ggactgaaag
    11041 acatcatcag taaattgggt aataacccta actttcaccg tttacgcatc ggaatcggtc
    11101 atccgggcga taaaaataaa gttgtcggtt ttgtgttagg caaaccgcct gttagtgaac
    11161 agaagttaat tgatgaagcc attgacgaag cggcgcgttg tactgaaatg tggtttacag
    11221 atggcttgac caaagcaacg aaccgattgc acgcctttaa agcgcaataa gtcgttgtct
    11281 gcggcatttt tgccgagtgc cgtgtataat aggcaaagtt atttccattt ctgcaatctg
    11341 ttagcaataa caggttgatt attaagatat taaggtgatt taaatcatgg gattcaaatg
    11401 cggtatcgtc ggtttgccca acgtcgggaa atctaccctg ttcaacgcgc tgaccaaagc
    11461 cggtattgaa gcggccaact ttccattctg caccattgag ccgaacacag gcgtcgtacc
    11521 aatgcctgat cctcgcctgg atcaactggc tgaaatcgta aaaccgcagc gtacgcttcc
    11581 cacgaccatg gaatttgtcg atatcgccgg tctggtaaaa ggcgcatcga aaggcgaagg
    11641 tctgggtaac cagttcctga ccaacatccg tgaaaccgaa gcgatcggtc acgttgttcg
    11701 ctgctttgaa aatgacaaca tcattcacgt ttccggcaaa gttaacccgg ctgacgatat
    11761 tgaagttatc aacaccgaac tggcgctggc agacctcgac acctgcgaac gtgcgattca
    11821 tcgcgtacag aagaaagcca aaggtggcga taaagacgcg aaagctgagc tggcggtcct
    11881 ggaaaaatgc ctgccccagt tggaaaatgc aggtatgctg cgcgcgctgg atttaagcgc
    11941 tgaagagaaa gcggctattc gttacctgag cttcctgacg ctgaaaccaa caatgtacat
    12001 cgccaacgtc aacgaagacg gttttgaaaa caacccatat cttgaccagg tgcgtgaaat
    12061 cgcggcgaaa gaaggttctg ttgtggttcc ggtttgtgct gctgttgaag cagacattgc
    12121 cgaactggac gacgaagaac gtgacgagtt tatgcaggag cttgggctgg aagagccggg
    12181 cctgaaccgt gtgatccgtg ccggttataa actgctgaac ctgcaaactt acttcaccgc
    12241 tggggtgaaa gaagtgcgtg catggaccat tccggttgga gcaaccgcgc cgcaggcagc
    12301 gggcaaaatc catactgatt ttgaaaaagg ctttatccgt gcacaaacca tctcgtttga
    12361 agatttcatc acttacaaag gtgaacaagg cgcgaaagaa gcaggcaaaa tgcgtgcaga
    12421 aggtaaagat tacatcgtga aagatggcga tgtgatgaac ttccttttca acgtctaatt
    12481 aatttattgt ctcatgaggt ttcgctaaat ctcatgaaga gcgcaaaagt atataaaatc
    12541 cacgcaattg cgtggatttt tcatttcacc gagtcctaat cattcttgta acatcctaca
    12601 cttttgtgct tattgcctat agtgaaccac gagaataatt aaagacataa gtgttcagat
    12661 gacggatatc tcattagcag tgatataacg taagtttttg tatcactaca catcagcccc
    12721 cttcacagct gatcaaaatt gatcacgctc caatatgctg gcaagatgaa aatgataacc
    12781 agtagcacaa taacaacgat aggccagaaa acccattgac caaaaatgaa tatcgatgtt
    12841 ttttgctttt tcttattagt aatgcaaaaa tggcgcatat tgtttttgtt atgcagatac
    12901 atcaagcgaa attcccataa ctaattagga ttattacaaa aaatcttcac caaatttttt
    12961 aaccgctgtt tgttgtaata tttttgccat atttatagta acgaattgga ataagtaacg
    13021 acaacttagg gaataactcg atataaactc acctttgtaa gggggatgtt aaatatatat
    13081 taattcctgt gttcctgatt ttgcccggta atagagaatt attctctgag aagctcatca
    13141 tgattgcacc atacaaatat catgatgata agaggatatt attcattgat ttaaacaaaa
    13201 tgacaaaatc catagaccca tagtctgggt actatatagg agtggtatgg gcatcaaaca
    13261 acacaatggg aataccaaag cagatcgtct cgctgaatta aaaatccgtt cgccctcaat
    13321 tcaactgata aaatttggcg ctattggttt gaatgcaatt atattttccc ccctgctgat
    13381 agctgctgat acaggaagtc aatatggcac caatattact attaatgatg gtgacagaat
    13441 tacaggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc cagcaggaaa
    13501 cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg acgcctctgg
    13561 atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta accgactcac
    13621 agtagatgtt gttggtcaaa cctctgccat cggcattaac ttaattggtg actataccca
    13681 tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca ttattgggca
    13741 tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta taggcctaac
    13801 catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca cgaccgatgg
    13861 aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg ctgcgcgttt
    13921 tacggctaca gacctgacaa tcgatgttca gggctacagc gccatgggga taaacgtaca
    13981 gaaaaactct gttgtcgatc tcggaacaaa cagtaccatt aaaaccaatg gcgataatgc
    14041 tcacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg atgtaactgg
    14101 agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg cagatagcca
    14161 tatttcttcc gcgcagggcg gtggcctcgt caccagtggt tcagacgcga taatcaattt
    14221 tactggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg gtgcctcggc
    14281 ccagacggca acggctgttg tcaacatgca aaataccgat attacagttg atcgtaatgg
    14341 cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag acagtttggc
    14401 tatcaccggc gcggcaggag ccaggggaat ttatgccatg accaacagcc agatcgacct
    14461 cacgagcgat ctggtcattg atatgagtac acccgaccag atggccatcg caacgcaaca
    14521 tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta tcaacggtag
    14581 cgttctttcc aaaggtgggc taatcaatct ggatatgcac cctgggtcgg tttggacagg
    14641 ttcctccctc agcgataatg tcaatggcgg aaaactggac gttgcaatga ataacagcgt
    14701 ctggaacgta acaattaatt ctaatctcga cacgctggcg ctgagccatt caactgtcga
    14761 ttttgccagc cacgggtcaa ctgccggcac atttgccaca ttaaacgtag agaacctgag
    14821 cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg gcgttaataa
    14881 taaaggggat ttattgaata tcagcgggag tagtgctggt aatcacgtat tggctatccg
    14941 caaccagggc agcgaggcca caacgggaaa tgaagttctg acagtggtaa aaaccactga
    15001 cggcgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc tgtacgatgt
    15061 gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg aaccgactcc
    15121 taatcctgaa cccacaccag ctcccgctca gcctcccata gtcaaccccg atcctacgcc
    15181 tgaacccgct cccacgccta aacccaccac gaccgcagat gctggcggca attatctcaa
    15241 tgtcggttac ttattgaact acgttgaaaa ccgtacgctg atgcaacgga tgggtgacct
    15301 gcgaaatcag agtaaagacg gtaatatctg gttgcgcagt tatgggggaa gcctggactc
    15361 ctttgccagt ggcaaactga gcggctttga catgggttac agcggtatcc agtttggtgg
    15421 ggataaacgt ctctctgatg taatgccgtt gtatgtcggt ctgtatattg gctcaacaca
    15481 tgcatcgccg gactatagcg gaggcgacgg taccgcacgt tcagactaca tgggaatgta
    15541 cgccagttac atggcacaaa acggttttta cagcgatctc gttataaaag catcgcgcca
    15601 gaaaaatagt ttccacgtac tggacagtca gaacaacggc gttaacgcca acggcactgc
    15661 gaatggaatg agcatctccc tggaagccgg gcagaggttc aacctgtccc ctactggtta
    15721 tgggttctat atagagccgc aaacccagct tacatacagc caccagaatg agatgactat
    15781 gaaggtgagt aatggcctca atatacatct gaatcactac gaatcgctgc tggggcgtgc
    15841 cagcatgata ctggggtatg acatcaccgc aggcaacagc cagctgaatg tctatgtgaa
    15901 gactggcgct atccgcgagt tttcagggga taccgaatat ctgttgaaca actcccggga
    15961 gaagtacagt ttcaaaggta atggctggaa taacggcgtg ggagtcagtg cacagtataa
    16021 caaacagcac acattctatc tcgaagcgga ttacacgcag ggtaacctct ttgatcagaa
    16081 gcaagtcaac ggaggatatc gcttcagctt ttaagtgcgg gatagacaag cccttaaacg
    16141 atgctgaaaa ttgttagcgt gcgccggaat gaaatacaga tcactctgat gtatcagaaa
    16201 tcgtttctgt ctttcatacc cggcgcttaa actgccctgc atcaatgcca tgttgcttca
    16261 ttttccgcca cagcgtagtg cggccgatcc caagtaaagc cgacatttcc tgaatgcgac
    16321 cgcctgtgac ctgggctgcg ttaataattg cctctttttc aacttccgca aatgacagac
    16381 tggtggaaag gcgggtggcg ctgacatcat ctgtcgcctg ctcggtaaac agatgttccg
    16441 gcaaatcact gacgcgaatg cgcccgttat cactactcag agcaagattc tcgatgacgc
    16501 tgtaaagttc aaaatcgttg cctggccatg cacaagaaac caggcgagcg agggcatcgt
    16561 catcaatttt cagccgcgta gagaagcgtt tttcaagact gcgtaatttg ttattcacca
    16621 gcgccggaat gctgccacgc cgcatacgca gaggcgggat ggtaatttca aatgcatgca
    16681 gcgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg aggtccgcgg
    16741 tcgttgttgc aatcactttg acatcaattg gtattaaacg ccgcgcatcc agtcgcgtga
    16801 taaccccctg cttgataacc tgaagcaaag cagactgtaa ctccaccgcc agatattcaa
    16861 tcttttcaag aaacagcgtg ccgccgtgtg ccagttccag ccgactcaga cggccatttt
    16921 cattgtccgt gcgatcgcca ccaataaatt cttccgccag cgcagcatca ccatataact
    16981 cacaattgac ggcgatataa ggacctgcag cacgctcgct ttcattatga attgcctgac
    17041 ttagcagtgc cttgcccacg ccctcttctc cacaaagcag gacaggaaag ctactgcgcg
    17101 ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt ggcatatgag
    17161 cgaaggtatg gctgactttt cctaattgac tggtcatcaa ctgccgcatc tgttccacag
    17221 gatggagcaa caaaataaag ctggttccct gcgtttctat tatcggttta agggttatca
    17281 ccgcatcaat aaactggtgc tggctttcaa aggttgcttc tacgtgtttg agcggatgtg
    17341 cctgttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg attgcccgtc
    17401 cctgacttgc cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcatta ataaattgca
    17461 aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac agggcattaa
    17521 gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt aaatttccga
    17581 cctcgcgggc gattgccaac gtcaacggca aatcagctgc ggtagtttgc tcaaccggac
    17641 acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc gttgcacaaa
    17701 aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc acggcctgac
    17761 cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg caatacgtgc
    17821 cgtcattgaa tcccagtgca cttagctgct gcaaggtttg cggatcgcca ttacggctga
    17881 gaatgcaggc ggtttcatcg aggataaaca gcgcacactc tcgcggtgcc atatattccc
    17941 aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc cgataaatag
    18001 aagcaaatgt cacgccctgg gcctgatgtg gtacgttcca tgtctcccgt ttcatcagct
    18061 tattgcatcg ctcccaggag gttgcaatta agggagatat gccccgacca tcgttgttaa
    18121 aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg caattttaag
    18181 tattaataca cgtaaaagtt tcaatatgga acattcatta aagcatatgt ttcactatga
    18241 aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt catcggcagt
    18301 cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg gagcaaaata
    18361 atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc aggactggcg
    18421 aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg agctgatgcc
    18481 cctgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga gccgatgcac
    18541 tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat tttcacctca
    18601 ccgacgcccg ataaaatctt tgaatgcgcc atgcaagttg atggcggcga aggtgtactg
    18661 ttgattatca aaaattacac cggcgatatt cttaactttg aaacagcgac cgagttactg
    18721 cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc ggtaaaagac
    18781 agtctttata ctgccgggcg acgcggcgtt gccaacaccg tattaattga aaaactcgta
    18841 ggcgcagcgg cggagcgtgg cgactcactg gacgcctgtg cggaactggg gcgtaagctg
    18901 aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc tgccgcgggc
    18961 aaaccttctt ttaccctggc ggataatgag atggagtttg gcgtcggcat tcatggtgag
    19021 ccgggtattg accgccgccc cttctcttcc cttgatcaaa ccgtcgatga aatgttcgac
    19081 accctgctgg taaatggctc ataccatcgc actttgcgtt tctgggatta tcaacaaggc
    19141 agttggcagg aagaacaaca aaccaaacaa ccgctccagt ctggcgatcg ggtgattgcg
    19201 ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta taaccgcctg
    19261 accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg cgcgtactgc
    19321 acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga cgaaacgctg
    19381 gcactctggg acgccccggt ccacaccccg gcccttaact ggggtaaata aggagaaagc
    19441 aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg atattttcag
    19501 caccgagagc gagtatctta ccggactgga tcgcgaaatt ggcgatgctg accacgggct
    19561 aaatatgaac cgaggcttta gcaaagtggt ggaaaaactc cctgctatcg cagataaaga
    19621 tatcggtttc attctcaaga ataccggtat gacgctgctt tccagcgtcg gtggtgccag
    19681 tggtccgctg ttcggtacct tctttatccg cgccgcacag gcgacccagg cacggcaaag
    19741 cctgacactg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg taatcagtcg
    19801 cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg tggtggaatc
    19861 gttacgtcag tccagcgagc aaaatctctc tgttccggtg gcgctcgaag ctgccagtag
    19921 catcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag gccgcgccag
    19981 ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg tgatgtttat
    20041 gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta aacctggtca
    20101 tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt cagatgttaa
    20161 tgagtgatag ttgtaaaatc gccattgccg cgggaattga cgatccacaa aatcccattg
    20221 gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgctgatgcc gaccatgtgc
    20281 tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg gaattgctgg
    20341 ctcccgagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa ggtacactgg
    20401 cagcaacggt cagcgcggcc tcgggggcgg atatcgacaa agttatcttt gacgccatgc
    20461 atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact gaaatctctg
    20521 cctcatgtcc tccgtacgat gaagaagccc gttctctggc ggttgtcata aagaaccgta
    20581 acggtctgca tgtacgtcca gcctcccggc tggtttatac cttatcgaca tttaatgtcg
    20641 atatgttgct ggaaaaaaac ggcaaatgcg tcacaccgga gagtattaac cagattgcgt
    20701 tactacaagt tcgctataac gatacgctgc gcctgattgc gaaagggcca gaagctgaag
    20761 aggcactgat cgctttccgt cagctggctg aagataactt tggtgaaacg gaggaagtcg
    20821 ctccacctac tctgcgtccc gttccgcctg tttcgggtaa agccttttat tatcaaccag
    20881 ttttatgtac ggtacaggca aaatcaaccc tgaccgtgga agaagaacaa gatcgattac
    20941 gccaggctat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa gcagaagcca
    21001 gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta gatgatccgg
    21061 aactgctggc ggcggcaagc gaactccttc agcatgaaca ttgcacggca gaatatgcct
    21121 ggcagcaagt tcttaaagaa cttagccagc aataccagca actggatgat gaatatctac
    21181 aagctcgcta tattgatgtg gacgatcttc tgcatcgcac cctggtccac ctgacccaaa
    21241 cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag aacatttatc
    21301 cttccacagt actgcaactg gatccggcgg ttgtaaaagg tatctgcctt agcgccggaa
    21361 gtccggtatc ccacagcgcc ctaatcgccc gtgaactggg gattggctgg atttgccagc
    21421 agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac gttaaaacgc
    21481 aacgtttcaa ccgtcagggt taagccaccg ttcggatggc atcgttctga tgtcatccgg
    21541 caatttaccc agcactgatg ttttttaact acctgttata ttttttacca atttatgatg
    21601 atttttcttc gctaatcgcg aaccaaatgt cgctggctgg ctaataaaca gacgttattt
    21661 gatcactcta tttcgccgtt atttatccct ttaattccct tttctaaaat gcctgacagt
    21721 tcgcagagtg agatttcgat catgcagcta gtgcgatcct gaactaaggt tttctgatac
    21781 ttgaataccg ttttattcgg tttcgccaaa ggagaatgat tgatgaaatc ccccgcacct
    21841 tctcgcccgc aaaaaatggc gttaattcca gcctgtatct ttttgtgttt cgctgcgcta
    21901 tcggtgcagg cagaagaaac accggtaaca ccacagccgc ctgatatttt attagggccg
    21961 ctgtttaatg atgtgcaaaa cgccaaactt tttccggacc aaaaaacctt tgccgatgcc
    22021 gtgccgaaca gcgatccgct gatgatcctt gctgattatc ggatgcagca aaaccagagc
    22081 ggatttgatc tgcgccattt cgttaacgtc aatttcaccc tgccgaaaga aggcgagaaa
    22141 tatgttccgc cagaggggca gtcactgcgc gaacatattg acggactttg gccggtatta
    22201 acgcgttcta ccgaaaacac cgaaaaatgg gattctctgt taccgctgcc ggaaccttat
    22261 gtcgtgccgg gcggacgttt tcgcgaggta tattactggg acagttactt caccatgtta
    22321 ggacttgccg aaagcggtca ctgggataaa gtcgcggata tggtggccaa ttttgctcat
    22381 gaaatagaca cttacggtca tattcccaac ggcaaccgca gttactattt aagccgctcg
    22441 caaccgccct tctttgccct gatggtagag ttactggcgc agcatgaagg cgatgccgcg
    22501 ttgaagcaat acctgccgca aatgcaaaaa gaatatgctt actggatgga cggtgttgaa
    22561 aacctgcaag ccggacaaca ggaaaaacgc gttgtcaaac ttcaggatgg tacccttctc
    22621 aaccgctact gggacgatcg cgatacgcca cgaccagagt catgggtgga agatattgcc
    22681 accgccaaaa gcaatccgaa tcgacctgcc actgaaattt accgcgacct gcgctctgcc
    22741 gctgcgtctg gctgggattt cagctcgcgc tggatggaca acccgcagca gttaaatacc
    22801 ttacgcacca ccagcatcgt accggtcgat ctgaacagcc tgatgtttaa aatggaaaaa
    22861 atcctcgccc gcgccagcaa agctgccgga gataacgcga tggcaaacca gtacgaaacg
    22921 ctggcaaatg cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca acaaggctgg
    22981 tatgccgatt acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc ggccgccctg
    23041 ttcccgctgt acgtcaatgc ggcagcgaaa gatcgcgcca acaaaatggc gacggcgacg
    23101 aaaacacatc tgctgcaacc cggcggcctg aacaccacgt cggtgaaaag tgggcaacaa
    23161 tgggatgcgc caaatggctg ggcaccgtta cagtgggtcg cgacagaagg attacaaaac
    23221 tacgggcaaa aagaggtggc gatggacatt agctggcact tcctgaccaa tgttcagcac
    23281 acctatgacc gggagaaaaa gctggaggaa aaatatgatg tcagcaccac cggaacgggg
    23341 ggcggcggtg gcgaatatcc attacaggat ggctttggct ggaccaatgg cgtgacgctg
    23401 aaaatgctgg atttgatctg cccgaaagag caaccgtgtg acaatgttcc ggcgacgcgt
    23461 ccgaccgtta agtcagcaac gacgcaaccc tcaaccaaag aggcacaacc cacaccttaa
    23521 ccagcgctta ctccgacaac gtcattcggc ttctccggat ggcgttgtcg gcagataaca
    23581 ccatagctat ggttttttct acacagacgg tttttcaagt cctggcggga agtattgcgc
    23641 aaatgtttgc catacgtggt tgccgaagat agcgtcattt ctacgggtaa gtgtttcact
    23701 ttcatcaccc ggcgatttac gccgacgttg gctatataaa gcatcaattg ctacctgaat
    23761 attgttacgg tgttcactca tcaacgtttt tttgctttct ggagttaaat taataatcac
    23821 ataatccaga cactccagag aatttagcaa ccaaaaaaag aattcagacc attcctcttc
    23881 gcgtaactga agcattgccg aacgggtatt ttggctaaat ctgccagaag gattgatcaa
    23941 ttctaaatgt tcaataagcg cagccaaaac cgcctcacta tccgcctcat ttttaacgcc
    24001 tgactggcta ctgtctaaaa tatttatttt ctgaatcaca ccaaaaacaa cgaaacaata
    24061 attaaaaaag gattctgttg ttaccgggaa ccgactttat cggatatcga agtgctatat
    24121 tgattttttt cacccgtaaa gcaggtgtcg agtatacgtt ctgatgtgaa agcggatagc
    24181 ggcatctgta tcgctatccg ccttatgaaa atcttattct cttcgcagga ggcggaatat
    24241 gcccagaacg agaatagctc ccaccaccgc caccaggaag ctgtgcagat taaaaccact
    24301 gatggagccg ccaatgccaa acatggtcgc cagccagccg ccgaccaccg caccgactat
    24361 cccgagaata caggtcagga aaaatccacc accatcacgc cccggcatga ttagcttggc
    24421 gataatgccg gctatcaggc caaaaataat ccaggcaata attcccatgt tcaggccctc
    24481 gtttcgatga atcggacgca agaaatgctt acgtcaacgc tctaagtata ggcaagggcg
    24541 ggatcgatgc tgggcgtatg tcggctgatt taagaacaac atgtaaaaaa tattcacgca
    24601 tttgtattaa gttttgttaa ctgtgaccga taaaccaaag acagtttgtc agtcaggagt
    24661 ttttccgcgt gagtcattac catgagcagt tcctgaaaca aaatccgtta gccgtcctgg
    24721 gcgtgttacg cgatttgcac aaagccgcaa ttcctttgcg tctcagttgg aatggcgggc
    24781 agctgatcag caaaatactg gcaataaccc cggataaact ggtgctggat ttcggcagtc
    24841 aagccgaaga caacatcgcc gtgctaaagg cacagcacat taccattacc gccgaaactc
    24901 agggtgcgaa agtcgagttt actgttgaac aactacagca gagtgaatac ttgcagcttc
    24961 cggcatttat caccgtaccg cctcccacct tatggtttgt acaacgacgc cgatatttcc
    25021 gcatctccgc cccactccat ccgccttatt tttgccagac caaactggcg gataacagta
    25081 cgttacgttt ccgcctgtat gaattgtcgt taggcggcat gggcgcatta ctggaaacag
    25141 caaagcctgc cgaattacaa gaaggcatgc gcttcgctca gattgaagtc aacatggggc
    25201 aatggggtgt ttttcacttt gacgcccagt taatctccat cagcgagcgc aaagtgattg
    25261 atggcaagaa tgaaaccatc accactcccc gtctgagctt ccgttttctt aacgtcagcc
    25321 cgacggtgga gcggcaatta cagcggatta ttttctctct cgagcgagaa gcccgggaaa
    25381 aagcggacaa agtgcgcgac tgatttacat cgcgtccagt gcctgctcaa gtttgtagat
    25441 atagcgcgga gcctgcggcg caggatgatt tcgcgctacg tgatcgagga actcgtcagc
    25501 atccaaatcg ttgattttgc tgatcgcctt tttccgatct gacgagaaag tccgtagcag
    25561 cgcacctgcc ccgttagcgt atgacaccac cagcgcatat tgcagtacct tcggatcttc
    25621 aatgcctgcc agcgggccgg tttccagaat attcaggtaa gccgccccca ttgaaatatt
    25681 acgctctgga tttttcagct cgctggtcgt cggctcacca ctccagccca tacggcgata
    25741 aacatcacgt ccggaggttg aagcttttaa ctgcatcaaa ccaatggcat tcgatttact
    25801 caccgcgttc ggattaccac ccgattcgat agcgataatc gccgtgatca attgtggatc
    25861 gacgccccag gctgcaccgg ctttctggct tattggcatc cactgcatcg cacgttgcac
    25921 cggaactttc gcgttccacg gcgggttcgt atagtcatgc tttgatgaac aacccgctaa
    25981 taacacaatc aaaaaggcaa accatctcaa tttcacttct tctatcctta tagcctggaa
    26041 tcgccgttga ggtgacaacg gcacacctaa gcggcatgat atacatttcg tttctgttgt
    26101 cagcaaggaa ttgccatgtc tctgtttcac ttaattgccc catcgggtta ctgcattaaa
    26161 cagcacgccg cgctgcgtgg tatccagcgc cttaccgacg aggggcatca ggtcaataac
    26221 gtcgaggtta ttgcccgtcg ttgtgagcgt tttgcgggta cggaaacgga gcgtctggaa
    26281 gatctcaatt cccttgccag actgactacc cccaacacca tcgtgctggc tgtacgcggc
    26341 ggttacggtg ccagtcgttt actggcagat attgactggc aggcactggt ggctcgccaa
    26401 caacatgacc cgttgctcat ttgcgggcat agcgatttta ccgccattca gtgcggtctt
    26461 ctggcgcaag gcaatgtcat cacctttagc ggccccatgc tggtagcaaa ctttggcgcg
    26521 gatgaactga acgcctttac cgagcaccat ttctggctgg cgttacgcaa taaaaccttc
    26581 accattgaat ggcaagggga aggcccgaca tgtcagaccg aaggcacgtt gtggggaggc
    26641 aatcttgcga tgctgatttc actgattggt acaccgtgga tgccaaaaat tgagaacggt
    26701 attctggtgc tggaagatat taacgagcac cctttccggg tcgaacgtat gctgttgcag
    26761 ctctatcatg cgggaatttt gccacgtcag aaggcgatca ttctcggtag ctttagcggc
    26821 agcacgccca atgattatga cgcaggttac aacctggagt cagtgtacgc gtttttgcgt
    26881 tcccgcctgt cgattccact cattaccggc ctcgattttg gtcatgaaca acgcacggtt
    26941 acgctgccgt tgggcgcaca tgccattctg aataacaccc gggaaggcac tcagttgacg
    27001 attagcggtc atcctgttct taaaatgtaa aaaaatcacg cttcagggct aagtaaaacg
    27061 ctgtctctgc cgctaatatg gtagggttta tataagagac agcgtaatca ggagtaaccg
    27121 accgttggat gccacaacaa taattagcct tttcatctta ggatccatcc ttgtcaccag
    27181 cagtatatta cttagttcat tttcttcccg tcttggcatt cctattctgg ttatttttct
    27241 ggcgatcggc atgctggcag gagtcgatgg cgtcggcggt atcccgtttg ataattaccc
    27301 cttcgcctac atggtaagta acctggcact ggcgattatt ttgctcgacg gcgggatgcg
    27361 cactcaggcc agttcctttc gtgtggcgtt aggaccggca ctgtcgctgg cgacgctggg
    27421 cgtgcttatc acctctggtt taaccggcat gatggcggcg tggctgttta atcttgattt
    27481 gattgaaggc ttattaatcg gcgctatcgt cggctctacc gatgctgcag cggtcttttc
    27541 tctgctgggt ggtaaggggc ttaacgaacg tgttggctcg acgctggaaa ttgaatccgg
    27601 cagtaatgat ccaatggcgg tctttctgac gattaccctg attgcgatga tccagcaaca
    27661 tgaaagcagt gtcagttgga tgttcgtggt cgatattctg cagcaatttg gcctcgggat
    27721 tgtcatcgga cttggcggtg gttatttact gctgcaaatg attaatcgca tcgccctgcc
    27781 tgccggatta tatccattgc tggcattaag cggcggtatt ttaatttttg ctttaaccac
    27841 ggcgctggaa ggcagcggta ttctggctgt ttatctgtgc ggttttctgc tgggtaatcg
    27901 gccgattcgc aaccgctacg gcatcctgca aaatttcgac ggcctcgcct ggctggcgca
    27961 aatcgccatg ttcctggtgc tggggctatt ggttaaccca agcgatctgc tgcctattgc
    28021 cattccggcg ctcattttgt ccgcatggat gattttcttc gcccgtcctc tttcggtatt
    28081 tgccggattg ctaccattcc gtggcttcaa tctgcgtgag cgcgtgttta tcagctgggt
    28141 gggattacgc ggcgcggtgc caatcatcct ggccgtgttc ccgatgatgg cggggctgga
    28201 gaatgcgcga ctgttcttta atgtcgcctt ctttgtggtt ctggtttcac tgctcttgca
    28261 gggaacatca ctctcgtggg cggcgaaaaa agccaaagtg gtggttccgc ccgtcggacg
    28321 tccggtgtca cgcgttggcc tggatattca tccggaaaat ccgtgggagc agtttgttta
    28381 tcaattgagt gctgataaat ggtgtgtagg tgcggcactg cgcgatttgc atatgccaaa
    28441 agagacgcgt attgcagccc tgtttcgtga taaccagttg cttcatccta ccggcagcac
    28501 ccgactgcgc gaaggcgatg tgttgtgtgt aattggtcgg gaacgcgatc ttccggcgct
    28561 cggtaaactg ttcagccagt cgccgccggt cgcgctggat caacgcttct ttggtgactt
    28621 cattctcgaa gccagcgcca aatatgctga tgttgcgctg atatatggcc tggaagacgg
    28681 gcgagaatat cgtgataagc agcaaacgct gggtgaaatt gtccagcagt tgttaggcgc
    28741 ggcaccggtt gtcggtgacc aggtagagtt tgccgggatg atctggacgg tggccgagaa
    28801 agaagataat gaagtgttga agattggcgt acgggtggct gaagaggaag ctgaatctta
    28861 aatttaatga agcatctatc gccggttgcg atgctttgct gaacgcagcc tgagcctgca
    28921 attgtttgtc gtccataatt taaccttcat ttgatgttga attgaacata tcaaaatcag
    28981 gcaattacac aaatctatgt acaggctcca tcaagggtaa agcgtgattt atctgaagtc
    29041 gagttcgagt caacaatgaa gcgtaagcgt tttatgtaga acgcctgtgc ggtgagttca
    29101 ggttccgggg aaatagtgtc cattctttta atggacacta tcatgggcaa tggctctgtc
    29161 acattaacaa ctacagttgc tgaccagccg gatggatgat tttttcgcaa ccagaagacg
    29221 ctgcctccga tccggcttac aacaagttac actgtcacaa ccgggacgcg taacgccagc
    29281 gcgcacatca actcatagcc caccgttccg gcagcggcgg cgacatcatc aattttgatc
    29341 tccttgcccc acagctcaac cggcgtacca atccccgcct gcgggcaagg cgttaaatcg
    29401 accgctagca tatccatcga gacggtcccc accgtcatgg tgcgcacgcc gtccactaaa
    29461 acaggggtac cggtaggcgc gtggcgcgga taaccgtcgg cgtaccctgc ggcgacaatg
    29521 ccgattcgct gttcatcgcg cgcagtatag cgaccgccgt agcccacacg ctcacccgct
    29581 tttagcgtct ggacaccaat aatctcactg cttagcgtca tcaccggacg taatccggta
    29641 ttggcgatat cacgccactg accggacggc gaagcgccat acaaaataat gccaggccga
    29701 acccagtcaa aatgcgcttc cgggtgccac agagtcgccg ccgaattgga caacgaacgc
    29761 cgacactcca gcccttccgc cgcctgctca atacgcgcca tcgcgctgga aattccatca
    29821 ggatgttccg cttcggcaaa atgcgacatc agggtcatct cgccaacatt cgccattgcc
    29881 cgcaactgct gccagacggt aagcacgcga tcgggctgga agcccaaccg attcatccca
    29941 ctgttcactt taagataaat atccaacggt gcttttagcc gcgcattttg cagtgctttg
    30001 agctgccagt tgctgtgtac gcaggtggtc aggcggtgct ggtcataaat ctccagatcc
    30061 tgagcatgga aaaatccttc cagcatcagg atcggcccct tccagccgcg ctcacgtaac
    30121 gttattgcct cttccaggtt aagtaatgca aagccatcgg tggccccgag cgcgctccag
    30181 atacgctcaa taccatgccc gtaagcgttc gcttttacca ccgaccagac gcgcgcgtgc
    30241 ggcgcggcct ggcggacaat ggacagattc tgttttaatg cctgcagatc gaggctggcc
    30301 tgtatcggac gggtcatctc gtttccttag ctgtgtgcgc catgtaaatg gcccggacgt
    30361 gatggcgtaa atccacggct gtagcgcgct acgcttagat cctcatatgg gatcgctggc
    30421 gtgcgaccag agagcagatc gcttaacaac tgaccggaac cgcaagccat cgtccagccg
    30481 agcgtgccgt gaccggtatt cagccacaga tttttaaagc gtgtacgccc gacaaccggc
    30541 gtaccgtctg gcgtcatcgg gcgcaaaccc gtccagaaag tcgcctgctc gacatgaccg
    30601 ccgcgtggat agagatcgcg aaccaccatt tccagcgttt cacgacgcgg ttgcaacagc
    30661 tcggtgttaa aaccaacaat ttccgccatt ccgccaacac gaatgcggtt atcgaaacgg
    30721 gtaatggcga ttttgtaggt ttcatcaaga atggtggata ctggcgcacc atcttcctgc
    30781 gcaattggaa tagtcagcga gtagcctttc agcggataca ccggaatatc aacaatgcct
    30841 ttgagcatcg ccgtcgagta agaaccaaac gccatcacat acgcatcggc cttaatcact
    30901 tcatcgccac acttcacgcc gtagatttgc tcgccgtcgc aaagcagttg gtcaacgggc
    30961 gtattaaagc ggaatttaac ccccgcctgc tctgccatcc gcgccagatt ctgggtaaac
    31021 agctggcagt ctccggtttc atcattgggc agttgcagac cacccgtcag tttgtgcgct
    31081 acttctgcca gcgcgggctc cacttccgcc aggcggctgg attccagcag ctgatacggt
    31141 acgccggcat cttccagcac ggcgatatcg cgggtcgcat tttcatactg ttgttcggta
    31201 cggaacagtt gcagcgtccc accctgacgc ccttcatact gaatattggt ttcggcgcgc
    31261 aatgctttca ggcaatcacg gctgtattcc gccagacgca ccatccgccc tttgttttcc
    31321 atgtagtggc tggtgtcgca gttacgtaac atttgccaca tccatttcaa ctgaaactgc
    31381 gtaccgtcga gacgaaccgc cagcggcgca tggcgctgga acatccattt gatcgctttt
    31441 aaaggcacac ctggtgccgc ccacggtgca gcatatccgg gggagatttg cccggcattc
    31501 gcggcgctgg tttccagggc ggcccccggc tcccgatcaa tgacggtgac ctcatgtcct
    31561 gcctgattta agtaccaggc gctggcaacg cctaccacac cacttcccag tatgacaact
    31621 cgcatagcca ctccgataac ggtagataca gtaaaagaat aataatctaa ttacatcttg
    31681 ataactcagt tgaaaatatt attcaacata cggctttttt atggtgagct ggctcacatc
    31741 tccctgactc tgcggcaata tggcgtcttt agtatctcct gctgaacgcc ctttttatcc
    31801 agcataaaat tccgttcaga agcggattag tggcactctg ataataagaa atcgcaaaga
    31861 aaaaaattct ccggcgacgc ggtttttaac aggtgttcta tgcttgaaat gaggtgctcc
    31921 acacagagag cgccgacaac aatgaggtgc gcgtatggcg acgatcgatt ctatgaataa
    31981 ggacaccaca cgtttgagcg atggacccga ctggacgttc gacctgctgg atgtttatct
    32041 ggcagagata gaccgggtgg cgaaactcta ccggctggat acctacccgc accagattga
    32101 agtgataacc tcagaacaga tgatggatgc ctactccagc gtcggcatgc caattaacta
    32161 tccgcactgg tcattcggta aaaagtttat cgagactgaa cggctgtata agcacggtca
    32221 gcaaggactg gcctatgaaa tcgtcattaa ctctaacccg tgtatcgctt acctgatgga
    32281 agagaacacc attaccatgc aagcgctggt gatggcacac gcctgctatg ggcataactc
    32341 tttctttaaa aacaattact tattccgcag ttggaccgac gccagttcga ttgtcgatta
    32401 tctgattttt gcccgtaaat atattaccga gtgcgaagaa cgttatggcg ttgatgaagt
    32461 agaacggctt ctggactcgt gccacgcgct gatgaactac ggcgtggatc ggtacaaacg
    32521 cccgcaaaaa atctcgctgc aagaagagaa agcccggcag aaaagtcgcg aagagtatct
    32581 acaaagtcag gtcaatatgc tctggcgtac cctgccgaag cgcgaggaag agaaaacggt
    32641 tgctgaagcg cgccgctatc cgtccgaacc acaagaaaac ctgctctatt ttatggagaa
    32701 aaatgcgcca ctgctggaat catggcagcg tgaaatcctg cgtattgtgc gtaaggtgag
    32761 ccagtatttt tatccgcaaa aacagactca ggtgatgaac gaaggctggg cgaccttctg
    32821 gcactatacc atccttaacc atctgtatga tgaagggaaa gtaacggaac gttttatgct
    32881 ggagtttttg cacagccaca ccaatgtggt cttccagccc ccctataaca gcccgtggta
    32941 cagcggcatc aacccgtatg ccctcgggtt cgccatgttc caggatatta aacggatttg
    33001 tcagtcgcca acggaagaag acaaatactg gttcccggat atcgccggtt ccgactggct
    33061 ggaaacgctg catttcgcga tgcgtgattt caaagatgag agttttatca gccagttcct
    33121 gtcaccgaaa gtgatgcgtg atttccgctt cttcaccgtg ctggatgacg atcggcataa
    33181 ttatctggag atttccgcta ttcataatga agaaggttat cgggagatcc gtaaccggtt
    33241 atcgtcgcaa tataacttaa gtaatctgga gccgaatatt cagatctgga acgtggattt
    33301 gcgcggcgac cgttcgctga cgctgcgtta cattccacat aatcgcgcac cgctggatcg
    33361 ggggcgcaaa gaagtgctga agcatgtgca tcgcctgtgg ggatttgatg tgatgctcga
    33421 acagcaaaac gaagatggca gcgtcgagtt gctggaacgt tgcccgccaa gaatgggaaa
    33481 tctgtaaaaa caacaaaaaa cccctcgttt gaggggtttg ctttttaaac ggaagggatt
    33541 atcgcccctg aatggctaaa tcacccggca gatttttctg catccggtgc caaatctcgc
    33601 cactctcatg cccatagcga cgcactgttt cgtacacctg atcgtgcgcg ccttcactgc
    33661 acaacgccga cagtttgtgg tagaagccga gcgccagact gcgcgcttcc ggattggcga
    33721 aatagtgacg accaatgcgc gtatacagtc ccttcatccc gttaaggatc agaccgtaaa
    33781 tcgggttgcc ggaagcaaac gccaggccgc ggaaaatgtt gtaatccagc tcggcaaagg
    33841 cgtcggcgtg atcggccact tcattagcgg tagccagcac ttcctgcgct ttatcgggat
    33901 gctgacgaaa cgcggtgcga ataaaaatag ttgaaatatt ggtacgcacc gacagcaaat
    33961 tatcaataag ctgcggcaca ctttcgtgat ccagtcgcgc cagtgtttca aggatattta
    34021 aaccagaggt ttcccagaaa ttattcacct tcgtcggctt gccatgttga atggtcaacc
    34081 agccatctcg tgccagacgc tgtaacactt cacgtaacgt agtacgcgtt acgccaatta
    34141 attctgaaag ttcacgttct gcgggcaaaa tagtcccggg agggaagcgg ttattccaga
    34201 tactttcaat aatgtactct tccgcgaaac ccgccgggct ttgcgcctta atgaccatag
    34261 tgagatttcc ataacacagc aaaacaaagt tggactcatc ataccagagg gctaactacg
    34321 ctgatagcag acaaaaagaa gaaaaaaggg atcaaggctt cattttccgc atctctttcc
    34381 ccttgactat caagacttgc ctcactcctg cctgaccgtt aagctttgcg tttaaacata
    34441 aaaacatcat tgattatttg taaggtaagg gaaaccatca tggagatctc ctggggccgc
    34501 gcgctatggc gcaatttttt gggccagtcc cccgactggt acaaactcgc cctcatcatt
    34561 ttcttaatcg taaacccgtt aattttcctc atcagccctt tcgtcgcggg ctggttgctg
    34621 gtagcggaat ttattttcac tctggcgatg gccctgaaat gctacccgct gctccccggt
    34681 ggtctgttgg ctatcgaagc ggtattcatc ggcatgacca gcgcggaaca cgtccgtgaa
    34741 gaggtggcgg caaatcttga agtcttgctg ttactgatgt ttatggtggc gggtatctat
    34801 tttatgaaac agctattgct gttcatattt acccgtttgc tgttaagcat tcgctccaaa
    34861 atgctgctgt cgctctcttt ttgcgtggcg gcggcgttcc tctccgcgtt cctcgatgcc
    34921 ttaaccgtcg tggcggtggt gatcagcgtt gcagtcggtt tttatggtat ttatcaccgc
    34981 gtagcctctt cccgtactga agataccgac ctgcaagacg atagtcatat cgacaagcat
    35041 tacaaagtgg ttctggaaca gttccgtggc tttctgcgta gcctgatgat gcatgccggt
    35101 gtcggcaccg cattaggcgg cgtgatgacc atggtgggcg aaccacagaa cctgatcatc
    35161 gctaaagcgg ctggctggca ttttggcgat ttcttcctgc gcatgtcgcc ggtgaccgtt
    35221 ccggttctga tttgtggcct gttaacctgc ctgctggtag agaagctgcg ttggtttggc
    35281 tacggtgaaa cgctaccgga gaaagtccgc gaagtgttgc aacagtttga cgatcaaagc
    35341 cgccaccagc gtacccgtca ggataaaatc cgtctgattg tccaggcgat tattggcgtc
    35401 tggctggtga ctgcgctggc gttgcatctg gcggaagttg gcttgattgg tttgtcagtc
    35461 attattctgg cgacatcatt gaccggtgtc accgatgaac atgctatcgg taaagccttc
    35521 accgaatctc tgccattcac cgcactgtta acggtgtttt tctcggtagt cgcggtgatt
    35581 atcgaccaac aactgttttc gccgattatt cagtttgtgt tgcaggcatc ggaacatgct
    35641 cagctgtcgt tgttctatat tttcaacggt ctgctgtcat ccatttcgga taacgtcttc
    35701 gtggggacga tttatatcaa cgaagcgaaa gcggcaatgg aaagtggcgc tatcacgttg
    35761 aagcaatacg agctgctggc ggtcgccatt aataccggta ccaatctgcc ctctgtcgct
    35821 acgccgaacg gtcaggctgc gttcctgttc ctgctgacct ctgcactcgc gccattgatt
    35881 cgcctctctt atggccgcat ggtgtggatg gccttgcctt acaccctcgt cctgacactc
    35941 gtcggactgc tctgcgtcga gtttacgctt gcccctgtaa ccgaatggtt tatgcaaatg
    36001 ggctggatag caacgctttg ataacaactt accgggcata taaatgcccg gtttgccttt
    36061 tcgcccgata attgtccaat tgcttcctat ttttgctgcc tcctggtggc gcagcgaatg
    36121 aattggttta aactgcgcac tctatgcata ttgcagggaa atgattatgt tgcgattttt
    36181 gaaccaatgt tcacaaggcc ggggcgcgtg gctgttgatg gcgtttactg ctctggcact
    36241 ggaactgacg gcgctgtggt tccagcatgt gatgttactg aaaccttgcg tgctctgtat
    36301 ttatgaacgc tgcgcgttat tcggcgttct gggtgctgcg ctgattggcg cgatcgcccc
    36361 gaaaactccg ctgcgttatg tagcgatggt tatctggttg tatagtgcgt tccgcggtgt
    36421 gcagttaact tacgagcaca ccatgcttca gctctatcct tcgccgtttg ccacctgtga
    36481 ttttatggtt cgtttcccgg aatggctgcc tctggataaa tgggtgccgc aagtgtttgt
    36541 cgcctctggc gattgcgccg agcgtcagtg ggatttttta ggtctggaaa tgccgcagtg
    36601 gctgctcggt atttttatcg cttacctgat tgtcgcggtg ctggtggtga tttcccagcc
    36661 gtttaaagcg aaaaaacgtg acctgttcgg tcgctaatcc attcggcgct cctgcgggag
    36721 cgcttttttc ctgccgctat gtttatttga cccgcagtaa atcagaactt cgcgttgtat
    36781 aacgtggtga aagcatggct cgcttcatct gccattgttg ctggatcccc tgcccggcaa
    36841 aatagagtgt tcctctgccc tctttagcat tcagtgtatc cattaccgtc atcaattgct
    36901 cactcccggg gcgcggtgcg ttgtcatcga ataaattgag ctgcgcgact ccctgactga
    36961 agaaatcccc cagcatcacg cccgcttttt ggtaacgatg gcccgcttgc cagatggcat
    37021 ccagagatcg cgtagcagcg ttaatgatat ccctgctgtc ctgagtgggc gtcagcagtt
    37081 ttaccgacgc gctattgccg taataaggtt cattgagcgc aaatggtgac gtcttaataa
    37141 acgtggagat aaaccgacaa tattgatgct cgctgcgaag tttttccgcc gcccgggcag
    37201 cgtaactaca aatggcctgc cgcatcgacg gataatccgt gatgcgttca ccaaacgagc
    37261 gggaacagat aatttcctgc ttcgtcggtg caaactcttc cagttgcaaa cagggttcgc
    37321 cgcgcagttc acgcaccgtt ctttcgagca cgacattaaa atgtttacgg ataaaccgga
    37381 tatctgtatc cgccaaatcg agaacggttt tgatccccat cgcgtccagt tttttgctga
    37441 tccgccgtcc aatcccccag acgtcatcca cggggagagc agacattaat ttacgctggc
    37501 gttccagatt tgataaatcc accaccccac ccgtctgccg gtgccatttt tttgccgcat
    37561 gattggcaag cttagccagc gttttggtct gggcgatccc cacaccaaca gtaagatggg
    37621 tacgttgtag caccgttgcg cgaatttctc tgccaaaatc agtcagatcg cgacaattac
    37681 gcacacctgt cagatcgcag aatgcctcat caatactgta aatctcgacg cggggcgata
    37741 gttcttccag cgtcgacatc acccgattgc tcatgtctgc gtaaagctca taattgctgc
    37801 taaagcaaac cacgccacag cgacgaaaca gatctttttg tttgaaccag ggatcgccca
    37861 ttttaacacc cagcgccttt gcctcagcgt ttcgggcgat aacgcaaccg tcattattcg
    37921 ataaaacaac caccggttta ccccataaat cagggcgaaa caccgtctca cagctggcat
    37981 aaaacgcgtt tacatcacag agggcaaaca tcagcgcatc gccttaacga cgtggatcac
    38041 cacaccaaag acatccagcg tatcttcgct actgatggta atgggcgagt acgcactgtt
    38101 catgggaata agctgtaccg tcgggcgtag ttgcaatttt ttcaccgtaa actcgccgtc
    38161 aacagcagcg atgacaatat caccatggct ggcggtaata gcgctatcga caatcagtaa
    38221 atcaccgtca ctaattccac catcaatcat agaatcacca cttgctttga cgaagtaagt
    38281 cgcgctggga tgctggatca acagttgatt cagatcgatg cgctgttcaa cgtaatctgc
    38341 tgccggtgaa ggaaagccac actgaacaag atcgctaaat agcggaaaag tcacaatttc
    38401 gcggagatcc gcaggcttga taaacaacat aataatctgc ctgaagttat actgttttta
    38461 tatacagtag tctgttcttg ccagcagatc aatactgatt caggctatca atatttgtcg
    38521 ctgtatagac tgctgatttt tcgttctctt atcctgtgct cacgtggcct tctggcgacg
    38581 acgctcatcc agcagaaatg aaaaatatca cctggtttaa aatagaatcg aagcattcgc
    38641 ccagaaaggc attctcatta aggtactgga tgacggcatc aagaaactgt gttctattgc
    38701 tgcgggcgta gttgaaggga aactgattcc agaattgaag aacaagttaa aatctgtgca
    38761 gaatttcttt accaccctgt ctaacacggt taaacaagcg aataaagata tcgatgccgc
    38821 caaattgaaa ttaaccaccg aaatagccgc catcggggag ataaaaacgg aaactgaaac
    38881 aaccagattc tacgttgatt atgatgattt aatgctttct ttactaaaag aagcggcaaa
    38941 aaaaataatt aacacctgta atgagtatca gaaaagacac ggtaagaaga cactctttga
    39001 ggtatctgaa gtctgataag cgattattct ctctatgtac tcaaggtata aagtttatca
    39061 catttaaaaa tacagacgtt tttaaagatt gagagttacc tgttgataat aaggtatcga
    39121 gtaactctca ttatgacaga gcgctttcaa aaacagggct acgaaatggc gagcatccag
    39181 acgtgtatta agaaagcccc catcaacatc tattaaattt atcactgcgc ccagtcaggt
    39241 ttatttaaga tatgatcctg ccagtcaatc acttctgatt ctttcactgc gatatgacgc
    39301 acagagatac gttcaccatg cattgccgct ttcgaaccag taagtagcgg atgccacaca
    39361 ggtaaacctt taccttccgc cagcaaacga taagcgcagg tcattggcag ccattcgaat
    39421 gttggcagat tttcacgggt taatttaatg cagtcgggtt caaactcgaa acgacgttcg
    39481 tagttccgac attgacaggt tttaatattg agctggcgac aggcgacgtt agtgaagtag
    39541 atttcgtcgg tgtcttcatc catcagttta tgcaggcaac actgaccgca accatcacac
    39601 aacgactccc attccgcatc gctcatttcg tccagggttt tactttgcca gaaaggtaca
    39661 tcgctcatca ggtgttccgc cattacgtta aaacgcacct tataaccagt ctggcacagc
    39721 gatgcaagtt ttgccgccgc tttcaggcgg caaaaagtat tacaaaacgc gagttgtcaa
    39781 agaatgccca tcgaaagtga ctgtcagctc atcaccgctt tgcaacgggc cgacaccatc
    39841 aggcgtgcct gtcagcacaa cgtcaccggc cttgagggta aaaaacttgc tcatataagc
    39901 gatcagcgga acgattttat ggatcatgtc cgcagtcgta ccttgctggc gttgctcgcc
    39961 gtttacgctc aggcccagcg ttgtattttg tgggtcgccg gtgaattccg ccgcgggaat
    40021 aaacccggaa agcggacaag agttatcaaa cgctttagcc ttttcccacg gctgcccggc
    40081 tttcttcatt tttccctgaa catcacgcaa tgtcagatcg agcgccacgc cataaccggc
    40141 aatggctttg cggacatgct cttccgtagc ctgacgcagc gtcgcgccaa tcaacaccgc
    40201 cagttcgact tcatgatgaa ctgaaccgaa atcggatggg atcgccaatg gctgccgcag
    40261 atcgcacagt gccgtttctg gtttaataaa cagcactggc tcttcgggca ctgcgctgcc
    40321 catctcttta atatgtttgg catagttact gccaacacag actactttac tcaccggata
    40381 atccagcagc gcaccttgcc agttgtgatg ttgatacata acgttcccct caccttatcg
    40441 tggatatgaa aacccgcgcc gacaaagata atgccaccgc cggatatcgg ttatttgtta
    40501 gtgtcgtctg ttttctgccc catgacggaa agatgttgct tcagcaaatc ttcgggtggt
    40561 ggcggtaact gcaaatagta accttgctcg gttaatgcct gtttaacctt ttcaatatcg
    40621 gcattgacca gtttcttccg cccatccagc ggcagaatca tcgctaactg aggctgacca
    40681 aaacctttca tcaattcctc aggaacacgc gaaaaatcgt cttttttttc gacatataaa
    40741 taggtctggt cacgcttgct gcttcgataa atcacacaaa acatactttt actctttaag
    40801 atgccaggtt ttggcataag ctaacatcaa tgaaacccgc gccacaagcc agccagctgt
    40861 agatttctac ggtttgttga gtccatgccc cctttataag taatcccccc ggcaaaaatg
    40921 ccgcgacggg attttaccga acgcaggaaa gccactggaa ttcccccgta agggattgtt
    40981 atattataac agttcatcgt actcattctg aacaggagac taccaatgaa aatcaagagc
    41041 atcagtaagg ctgtattgct gcttgcactg ctaacctcca cgagctttgc tgcgggtaag
    41101 aatgtcaatg ttgagttcag aaaaggccat agcagcgctc agtactccgg cgaaataaag
    41161 ggatacgatt acgatacata taccttctat gccaaaaaag gtcagaaagt gcatgtgagt
    41221 atctctaatg aaggcgctga tacttacctc ttcgggccag gaatcgatga ttctgttgat
    41281 ttatccagat attccccaga actcgatagt cacggacaat actcactccc tgcttctggc
    41341 aaatatgagc tgcgagtgct gcaaacccga aacgacgccc gtaaaaacaa aactaaaaaa
    41401 tataacgtcg atattcaaat taagtaaatg tcaaccgacg gggactctcc ccgtcataaa
    41461 cgttaacgcc ccactcctct ctgtcgctca agaggttaat gacgaatagc agacaatgcc
    41521 gaatagaaca taatgtaaac aaggcatcac tcacgacaca taaataacat ctttgtggtg
    41581 gtttgattga tttttccact ccgcttacca tccacaccaa aataacgatc atcacgacat
    41641 aatttttctt taacatcgca aaatacaccg ttagcgaatg taaattcgct gggatcgtag
    41701 ccttgtaatg attgtaagtt ttcagcggct ttttcgccga gatatttcct tgttaactct
    41761 ggggaaatgc catctgagtt cacacaaata tacgggtcgc aaacaacgcc gcacgccgga
    41821 gattgaagtc cgccagcgtc atttccagac gccattgatg agaacatcat ccccacacct
    41881 gacaatccga tataaaaaat acgcatcata ttcatagcac atcctgataa tccaaccgta
    41941 caacacttat gctcatttca tcatcaatcc gtaatcaaaa ccgctgacct ggacgagtta
    42001 cgatgacctg acagaagtat cagacgtctt ctgcggtttc tatgatagag caattctgtg
    42061 gcggatatta agataacggg tatctgttct ttaatgatgc tgcaaaaaat ggaaagttaa
    42121 gaaagcctgg catggtgaaa aaatacactt gcctcaatac tggatattta aacaggtcta
    42181 aagatgctgt cacgattagg gtgcggaaaa aggaagcgta aataagaaca atcaaaagta
    42241 cgacggcaat gggttgattg acaagggtat tttttaagct atgaatcagc gccatttatc
    42301 acagaataga cttttactct gaataaatgg gagggtgact tgcctcaata taatccagac
    42361 tataacatgc cttatagtct tcggaacatc atcgcgcgct ggcgatgatt aatagctaat
    42421 tgagtaaggc caggatgtca aacacgccaa tcgagcttaa aggcagtagc ttcactttat
    42481 ctgtggttca tctgcatgag gcagaaccta aggttatcca tcaggcgctg gaagacaaaa
    42541 tcgctcaggc ccccgcattt ttaaaacatg cccccgttgt actcaacgtc agtgcactgg
    42601 aagacccggt aaactggtca gcgatgcata aggcggtttc ggcaaccggt ttgcgggtta
    42661 ttggcgtaag tggctgcaaa gatgcgcaac ttaaagccga aattgaaaaa atggggctgc
    42721 ctatcctgac ggaaggaaag gaaaaagcgc cacgtccagc tcccgcaccg caggctccag
    42781 cgcaaaatac aacgccggtc acaaaaacgc gtttaataga taccccggtg cgttccggtc
    42841 agcgtattta tgctccacaa tgtgatctga ttgttacaag ccacgttagc gctggggccg
    42901 aattgattgc cgatggtaac attcatgtct atggcatgat gcgcggtcgt gcgctggcag
    42961 gggcaagtgg tgaccgggaa acgcaaatat tttgtacgaa cctgatggcg gaactggtgt
    43021 ccatcgcagg tgaatactgg ctgagtgatc aaatcccagc agaattttat ggcaaagcgg
    43081 cgcgactgca gttagtcgaa aacgctttga ccgttcaacc gttaaattga tcccttttta
    43141 acaaggaatt tctatggcac gcattattgt tgttacttcg ggcaaagggg gtgttggtaa
    43201 gacaacctcc agcgcggcca tcgccactgg tttggcccag aagggaaaga aaactgtcgt
    43261 gatagatttt gatatcggcc tgcgtaatct cgacctgatt atgggttgtg aacgccgggt
    43321 cgtttacgat ttcgtcaacg tcattcaggg cgatgcaacg ctaaatcagg cgttaattaa
    43381 agataagcgt actgaaaatc tctatattct gccggcatcg caaacacgcg ataaagatgc
    43441 cctcacccgt gaaggggtcg ccaaagttct tgatgatctg aaagcgatgg attttgaatt
    43501 tatcgtttgt gactccccgg cagggattga aaccggtgcg ttaatggcac tctattttgc
    43561 agacgaagcc attattacca ccaacccgga agtctcctca gtacgcgact ctgaccgtat
    43621 tttaggcatt ctggcgtcga aatcacgccg cgcagaaaat ggcgaagagc ctattaaaga
    43681 gcacctgctg ttaacgcgct ataacccagg ccgcgtaagc agaggtgaca tgctgagcat
    43741 ggaagatgtg ctggagatcc tgcgcatcaa actcgtcggc gtgatcccag aggatcaatc
    43801 agtattgcgc gcctctaacc agggtgaacc ggtcattctc gacattaacg ccgatgcggg
    43861 taaagcctac gcagataccg tagaacgtct gttgggagaa gaacgtcctt tccgcttcat
    43921 tgaagaagag aagaaaggct tcctcaaacg cttgttcgga ggataagtta tggcattact
    43981 cgatttcttt ctctcgcgga agaaaaacac agccaacatt gcaaaagaac ggctgcagat
    44041 tattgttgct gaacgccgtc gcagcgatgc agaaccgcat tatctgccgc agttgcgtaa
    44101 agatattctt gaggtcattt gtaaatacgt acaaattgat cctgagatgg taaccgtaca
    44161 gcttgagcaa aaagatggcg atatttctat tcttgagctg aacgtgacct taccggaagc
    44221 agaagagctg aaataagccc gctgtaaaag cgcatttatc ttcaaggcag agttatctct
    44281 gccttgagtt tttcatccct ctcatccacg ttgtggtaaa gcggcgagta ttcttgctga
    44341 tactcctcat tgctatttcc acctccccct ttacacctta aggctgttcc cacgcacaaa
    44401 acaaaatgtt tatgcctggt tgagtaaata accttattgt tagtatggat atactgggag
    44461 atgataaccc cgcaatttca ttattcattg ttctcccata ggatgaggtt tcccgccgta
    44521 actggcgggc tttttttgcc ccagaatttt ccctttcaac atcctgtaaa cgaaaactgc
    44581 gccgaagcgc agtttaatcg ttgatggtca gaacgaccag ttcacaccag ccaccgcgtt
    44641 ccacggggat tccacaccgg caccatggct ataccccacc ccgagatgtc cgctaaacgt
    44701 actgctgaat gaggctttaa tagctgcctg gtatattcca cgtctgcccg acaaatcatt
    44761 gacgaaatta ccgtcattat tcaccttcac ccggttatca tcgacaaatt ctttgcgcac
    44821 agccgccttc agccagggtt catttccata ccgttcccca ggcgcatgtt gtaactcagc
    44881 gttgcaccca gttcacgata tatactgcgg gtatcgactg atttcgattc catgccattg
    44941 gataaatgat agtagcgtct aagtgaccta agctctgctg caacatcagc ccgcaggcac
    45001 cagaccaggg gattcatcct gaagagacag cgcaggtgta ttgtgttcac cgctcaccaa
    45061 agacatcatg atgaaatgat gatatcccgc ataagaaaga ggcatttttt acacgcagtg
    45121 cgctgaagtg tagttggata aaaaatcact cttttctgat aatactgtcg tacgcttatc
    45181 tttcgacaat gaggccgtcg gcaaaataaa atgactaatg taatcgttgc tgatgaatat
    45241 cttctgctca cataaaaatc acacaataat attgataaat cagattatct tacctttaca
    45301 acattgcgtt aacagtcaat gacacttctt gaataaaaat attgtcaatc aatgagttat
    45361 tcctcattta agattattct cattcacgta aaaaacataa ttcctatcat cacagccacg
    45421 ctcattttga attatctgaa taatagcgag cgttctttag ttaagcagat cttaattgaa
    45481 gacgaaatta ttgtgtgtgc aacttactta ataccggaca tctaagttga agaaaaaaat
    45541 acttgccttc gggcttatct cagcattgtt ctgctccacg ccagcgatgg cggatatgaa
    45601 ccgcaccacg aaaggtgcat tgctaggcgc aggcgttggt ttacttactg gcaatggtgt
    45661 taatggcgta ctgaaaggtg cagctgttgg cgctggtgtt ggtgcagtaa cagaaaaagg
    45721 ccgcgacggt aaaaatgcgc gtaaaggtgc aaaagtgggt gccgccgttg gtgctgtcac
    45781 tggcgttctg accggcaatg gcctcgaagg ggcgattaaa ggtgccgtga ttggcggtac
    45841 gggtggtgca attctgggta aaatgaaata aggaatgatg atgaaaaaat ttgctttact
    45901 ggcgggcctg tttgttttcg caccaatgac ctgggcgcag gactacaata ttaaaaacgg
    45961 tttgccgtca gaaacctata ttacttgcgc agaagccaac gaaatggcaa aaactgacag
    46021 cgcacaggtt gcagaaattg ttgcggtaat gggtaatgcc agcgttgcca gccgtgattt
    46081 aaaaattgag caatctccgg aactgagcgc caaagtggta gaaaaactga atcaggtttg
    46141 cgctaaagac ccgcaaatgc tgttgatcac ggcaattgat gacaccatgc gtgctatcgg
    46201 caaaaaataa tatttcatat gtaccgcgtc gatgaatacg acgcggtttt ttagctaacg
    46261 gcgggatcag aacttatatt tcaggcccac catcactgcg gtgtcattgt agccattatc
    46321 acccagctgc acgcccacat tcccccagac gctggccgct ggattcaggt tgccatttac
    46381 gccaagttta atttccccca aattactcgc cccatcttgt tcgactttca caccgttcat
    46441 tgagacagca aaatctttac tgttatggat ccagttcatt tcaacaaaag gttcaaagtc
    46501 gtcaccgtgc ggaccgctgt ttttctcctg agtacgaata aaggtgcgga agccgagtcg
    46561 ggtctggatg ttgttatttg cgtcattctc aatacgtgag ccattggctt ccgtgtgcgc
    46621 attatgatcg acgcccatcc agatagcctg tgcctgtggc tggaagatcc acgtatattt
    46681 cgccgcttcc agaccaaagg atttattcag ggcaaaaccg taacccgctt ccagcgagac
    46741 ggttgcacct ttcgcgctat aggattcata ggctaattca tcccctttca ccgagttttt
    46801 aaaccagcta tattgcgccc aggagtcaat gtatgcgcct tttttactga tgtcatcggc
    46861 aaaccaggtg gcatacagcc ctgcgctata gcctctgaca ctccctttcg aacgataatc
    46921 cgacacgctg gaatgagtta agttgtagtc gcgggcataa ccagccatca ctcccaaacg
    46981 ccaactgtca ctatcggtaa aaccaccggt taacagatcg ccccctaact gcgagacgta
    47041 gcgatgcgag gtcgttctca actgtccgtt gctgtcacgc caggcattat gcccgccaat
    47101 ttgacgtagc caaagtcggc ttgaacgctc ctgttcagtt acaggatcga tgtagcgcgt
    47161 ttcacccgca cggtcgttca gacgcataac aaaaagagtg ttagcagctg ccaggttagc
    47221 gacgtaactt ccagcttcgg ggcgcaggac gggggtaggc gtttctgttg attctgaatt
    47281 tccctctgag ctgttatcct cctcattcac tgactgataa cttgtcaaat accagttttt
    47341 gccttgttgt tttagttgat attcgaaagc tccagctgca atccttgcat caccttgtct
    47401 gaaggcatct gctggagatt catcgccatt cacagttatg agttcgatcc cgtttagcgt
    47461 ggccgcgccg cttcctccag catttttcac atagacggta gttgaacccg aagtattacc
    47521 ctcaacaata agtctgtccg tagccgattt atcatcacct aatacggtgc ggatattaag
    47581 ctgaccatta ttaccgacat aattaccttt tacggttaat gtttttcctt gggtaaatgt
    47641 ttgaccatca tcatacgaaa gattgataat actgtctgaa ttagtcagat gggtaacaac
    47701 ggagtcatct ttcatattcc agattgtatt ttctgaaagc ttcagcgtgg ttgtgccgct
    47761 gacgagttta gtcgctccat ttagaataga gtcggacgaa attatgttta agtcacctcc
    47821 agtattatta accagaacat tattaccagg tgtgataata cttcgattaa ggaaacttac
    47881 atccgttgta gttttgttaa catcaatact atccaaagca cttgaaagtt caacacttaa
    47941 tagtgctatt ttggcttgag aatcgatatt tgatgcatca attgatattc ctttatcttg
    48001 ttttgatacc gcggtgacat tggaaaaaaa taactctcca ccattgtata tttttgcaat
    48061 gggggcttta gtgcctgttg ttgtaataga actatttttt atatcaatgt taccttgtcg
    48121 aatagcaact gcataacttc catcagccac agtttcaagt gccacataat ctaaattaat
    48181 atatgctttt ttcccattag cataagcacc atagctatta atgccattag ttgttatgga
    48241 accaccattc atttttaaat cagcacggct aataacttcg taactgttag aatcactaac
    48301 ttcatcatct acagcatcga cacatgttcg agactctcca ggacttgaac atgcaagaac
    48361 acctactgca ttttccccag aggtaacaat gttcgtttta tctgtggttt caagacgggt
    48421 gttatttacc atcttttcag aatcattaac ctggcttaaa agtcccgctg aatatgcccc
    48481 acccgactcg actgaattat caccattcat aaagatggtg ccaccaaatt cactgactac
    48541 tgcgctggct gttatccctc cattttcttt gtcagatacg cttccagagg tcttaatatc
    48601 gctatcgctt atatataata ctttgttttg actatcaaca gaaataccat aagatccatc
    48661 accactagtc atcacactgg cattatttat atatccagtt acaccgtcat ccactttcac
    48721 agcatggctt tcgtcttcag tggttagata ctctccatta cttatcggat tattatcttt
    48781 gatttcaatc ggcgattctt catcagtcat ataggaaatt atactataat catccaacaa
    48841 ttgtgttgca tatgcattac ccaccgccag agcaataagt cccaaactaa acccggggag
    48901 ttttttcagt ttcatagatt aaccatccat tcattggatt aacaaacatt cctggtcaaa
    48961 taacctatga gctcagatta ccaaaacgga gggtaaagac aaaaacaaaa gcacaaaaaa
    49021 tttaaataga taaacagcga caatatataa atgataaata agtcggcatt atcatttttc
    49081 accttatttt catgacattg atcactttga ggtgacgatt ttgtttaaca aataactctt
    49141 cgacatcaaa attgttaata gttgcgatga ggattatttc ggtgatatat aaaaacaacc
    49201 gtgataacac cagggttgac aactatttgc gcaataatgc ctttccttca atgatccagg
    49261 cgttcactgc cagcaaccca ttcagccgca tactgacagt gagaaactga aagattattg
    49321 ctgatccctg tggttgacga gtctttctct taatcgatta accagattga tcgccagcca
    49381 aacaagggca ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tactgggttt
    49441 tgcaataagg atgaccagga gggtgatcag cataaatgca atggaaaata tgatatagag
    49501 agtgaaataa tccagattat tactattgtt catgctacta atggcaatcc tgtcaaaact
    49561 ttcaaacaat tatagcaaac aaaataatga acaatagtaa caagaggcaa cgagctaatt
    49621 agctgtctat aattacaccg cactatggcc ttactgatca attttcactc aaccacaacc
    49681 ttcaccttcg gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag
    49741 aaataataac cctgctgaaa tgtgatatta ttttggttca gatagtcaag ttgttctttc
    49801 gtttcgacac cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa
    49861 tccagaattc ggattgattc tgggtctgcg tttacgcggc caacgaaact gtgatcaatt
    49921 ttgatataat caatatgcag gtcatgaaga taagaaagcc ctgagtagcc agtaccgaaa
    49981 tcatccagcg cgatgacaaa accattttca tgcagtatgt tcaaccgctg aaccagactt
    50041 tcatcaacat tcaatggctc acgctcggtg acttccagaa caaggtttaa atcgcggcgg
    50101 gtaaaactgt cacggaaatt taaacactcg tcgacaaacg tcggcgaaat aatatgcgaa
    50161 gcgctaaaat taattccaat atgaaaacct tccggcaatt tactcgcgat agcgttcatc
    50221 tgtctggcaa cctgattcat caggctttgc gtaagcggaa cgattaatcc ggatttttca
    50281 gcaagtggaa taaatgccgc gggtgatata tatccaccgt gaggttgttt ccagcgggct
    50341 aacacctcaa ctccccgcaa tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg
    50401 acgatctccc cattatttat tgcccgtcgc aggatctctt caggcgttgc gcttttattg
    50461 aaatatctat caagcaggaa ggcagcggcg caggcgatta acagaataaa tattaaaatc
    50521 ccaaagcctt tatcgataag tctgtagaag ctaaagagtg gcggcgggtt aaagataata
    50581 gtgaaatgat attttgtgga gtgaaccttc tcggtatagg gataatgtcc attaagcgtc
    50641 atcacatcac ccgttggtcc aataatgtca tccgccacgc gtaatacata ccttacccct
    50701 ttcaagggta cattaagtgc cccacgaata tgctgatcgc ttatcgtaac caaaatgcgc
    50761 gtatctgcaa attggttctg atagagcagg ataggtaatc tattaacggt gtcgattgct
    50821 ggagccaaca gtaaattact gtccgggaaa acaggaatac gagacaacag gacccgattc
    50881 ccaggcaggg atgtacacca gactattccc tgtttgagaa tgatgattgt gcgcaggtga
    50941 ggtttcagag ctgcttcagt gccaagctga tattgctcct cgaggttgca ttccttcccg
    51001 gcaatgttaa ccgctgtttg agtcgcttgt gacgcttctt caagtatatg attgatattg
    51061 ttggcggcat atctcgctcc cgccaggtac tctgcttttg cggaatacca gagctggatg
    51121 ttcaaaatag cgacaccggt aataaacaag catatcgcaa cgaggatggg tatgagtgta
    51181 ttgcgcattt gcttgtcccg gccaaagggt ttaatacatg aaaatgccat tcattcgctc
    51241 gcgctggcat ctacagagag catggtagag agcataagaa atatccacgc cgaagataaa
    51301 attttctcca tcttgatcta acccattaat tattgagcat aatgtaggca gtacaaaata
    51361 agttaggcgg gatatcaggc gtcaagaatg gagagcgaga actctccatt cttgacacct
    51421 gatattgcgg acataataag aaagcataac gcctgaaatg ctcactttgc atcagcatgg
    51481 tgatacagct gatgtttatt ctaaaacctt actcaagttc taagagagca cggattccct
    51541 gtcattaata atgatatccg ttagtgtagt cttatgcatt tcaatgtact gcaatatttg
    51601 cagatagaga tcagaaagaa tcacactggt ttcatctttt aaacgcaagc gtaaagcctg
    51661 atcaataata tgactatcaa catacccatg cgtcatgaac acctccctct cgagattt
//
LOCUS       DAOFQD010000019        51130 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.019, whole genome shotgun sequence.
ACCESSION   DAOFQD010000019 DAOFQD010000000
VERSION     DAOFQD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.019"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            169..474
                     /locus_tag="QBF46_001173"
     CDS             169..474
                     /locus_tag="QBF46_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDQ6673383.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSSQDGAPAEPQ"
     gene            673..3099
                     /locus_tag="QBF46_001174"
                     /pseudo
     CDS             673..3099
                     /locus_tag="QBF46_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
     gene            3160..5583
                     /locus_tag="QBF46_001175"
     CDS             3160..5583
                     /locus_tag="QBF46_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDQ6673384.1"
                     /translation="MKIHTTEALMKAEISRRNLMKTSALGSLALASSAFTLPFSQMVR
                     AAETPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKIISIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWVPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            5594..6211
                     /gene="dmsB"
                     /locus_tag="QBF46_001176"
     CDS             5594..6211
                     /gene="dmsB"
                     /locus_tag="QBF46_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDQ6673385.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            6213..7067
                     /locus_tag="QBF46_001177"
     CDS             6213..7067
                     /locus_tag="QBF46_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDQ6673386.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGVGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLFSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            7110..7724
                     /gene="dmsD"
                     /locus_tag="QBF46_001178"
     CDS             7110..7724
                     /gene="dmsD"
                     /locus_tag="QBF46_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDQ6673387.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     ILALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7883..9175
                     /gene="clcB"
                     /locus_tag="QBF46_001179"
     CDS             7883..9175
                     /gene="clcB"
                     /locus_tag="QBF46_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDQ6673388.1"
                     /translation="MHHLHIYPDLRTMFHRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TEGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(9128..9823)
                     /gene="bioD"
                     /locus_tag="QBF46_001180"
     CDS             complement(9128..9823)
                     /gene="bioD"
                     /locus_tag="QBF46_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDQ6673389.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9948..11168)
                     /gene="mlc"
                     /locus_tag="QBF46_001181"
                     /gene_synonym="dgsA"
     CDS             complement(9948..11168)
                     /gene="mlc"
                     /locus_tag="QBF46_001181"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDQ6673390.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(11303..12196)
                     /locus_tag="QBF46_001182"
     CDS             complement(11303..12196)
                     /locus_tag="QBF46_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6673391.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            12303..13556
                     /locus_tag="QBF46_001183"
     CDS             12303..13556
                     /locus_tag="QBF46_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6673392.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            13953..14288
                     /locus_tag="QBF46_001184"
     CDS             13953..14288
                     /locus_tag="QBF46_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDQ6673393.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            14381..14464
                     /locus_tag="QBF46_001185"
     CDS             14381..14464
                     /locus_tag="QBF46_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673394.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            14564..15385
                     /locus_tag="QBF46_001186"
     CDS             14564..15385
                     /locus_tag="QBF46_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDQ6673395.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(15424..15753)
                     /gene="mdtI"
                     /locus_tag="QBF46_001187"
     CDS             complement(15424..15753)
                     /gene="mdtI"
                     /locus_tag="QBF46_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDQ6673396.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(15740..16105)
                     /gene="mdtJ"
                     /locus_tag="QBF46_001188"
     CDS             complement(15740..16105)
                     /gene="mdtJ"
                     /locus_tag="QBF46_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDQ6673397.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            16517..17551
                     /gene="tqsA"
                     /locus_tag="QBF46_001189"
     CDS             16517..17551
                     /gene="tqsA"
                     /locus_tag="QBF46_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDQ6673398.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(17576..18964)
                     /gene="pntB"
                     /locus_tag="QBF46_001190"
     CDS             complement(17576..18964)
                     /gene="pntB"
                     /locus_tag="QBF46_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDQ6673399.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(18975..20507)
                     /gene="pntA"
                     /locus_tag="QBF46_001191"
     CDS             complement(18975..20507)
                     /gene="pntA"
                     /locus_tag="QBF46_001191"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDQ6673400.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            21031..21975
                     /locus_tag="QBF46_001192"
     CDS             21031..21975
                     /locus_tag="QBF46_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ6673401.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            22161..23543
                     /locus_tag="QBF46_001193"
     CDS             22161..23543
                     /locus_tag="QBF46_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6673402.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            23580..24302
                     /gene="folM"
                     /locus_tag="QBF46_001194"
     CDS             23580..24302
                     /gene="folM"
                     /locus_tag="QBF46_001194"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDQ6673403.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASVWMAEKPDAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(24299..24634)
                     /locus_tag="QBF46_001195"
     CDS             complement(24299..24634)
                     /locus_tag="QBF46_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDQ6673404.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            24763..25482
                     /gene="rstA"
                     /locus_tag="QBF46_001196"
     CDS             24763..25482
                     /gene="rstA"
                     /locus_tag="QBF46_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDQ6673405.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            25486..26787
                     /gene="rstB"
                     /locus_tag="QBF46_001197"
     CDS             25486..26787
                     /gene="rstB"
                     /locus_tag="QBF46_001197"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDQ6673406.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            26863..27792
                     /gene="tus"
                     /locus_tag="QBF46_001198"
     CDS             26863..27792
                     /gene="tus"
                     /locus_tag="QBF46_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDQ6673407.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(27789..29192)
                     /gene="fumC"
                     /locus_tag="QBF46_001199"
     CDS             complement(27789..29192)
                     /gene="fumC"
                     /locus_tag="QBF46_001199"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDQ6673408.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRGSELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNNHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(29335..30981)
                     /gene="fumB"
                     /locus_tag="QBF46_001200"
     CDS             complement(29335..30981)
                     /gene="fumB"
                     /locus_tag="QBF46_001200"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDQ6673409.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEWQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            31180..32355
                     /gene="manA"
                     /locus_tag="QBF46_001201"
     CDS             31180..32355
                     /gene="manA"
                     /locus_tag="QBF46_001201"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDQ6673410.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            32456..33964
                     /locus_tag="QBF46_001202"
     CDS             32456..33964
                     /locus_tag="QBF46_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDQ6673411.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(34009..35274)
                     /gene="uidC"
                     /locus_tag="QBF46_001203"
     CDS             complement(34009..35274)
                     /gene="uidC"
                     /locus_tag="QBF46_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDQ6673412.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(35313..36686)
                     /gene="uidB"
                     /locus_tag="QBF46_001204"
     CDS             complement(35313..36686)
                     /gene="uidB"
                     /locus_tag="QBF46_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDQ6673413.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLN
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(36683..38494)
                     /gene="uidA"
                     /locus_tag="QBF46_001205"
     CDS             complement(36683..38494)
                     /gene="uidA"
                     /locus_tag="QBF46_001205"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDQ6673414.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQACNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(38885..39475)
                     /gene="uidR"
                     /locus_tag="QBF46_001206"
     CDS             complement(38885..39475)
                     /gene="uidR"
                     /locus_tag="QBF46_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDQ6673415.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(39704..40471)
                     /gene="hdhA"
                     /locus_tag="QBF46_001207"
     CDS             complement(39704..40471)
                     /gene="hdhA"
                     /locus_tag="QBF46_001207"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDQ6673416.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMEFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(40583..41611)
                     /gene="malI"
                     /locus_tag="QBF46_001208"
     CDS             complement(40583..41611)
                     /gene="malI"
                     /locus_tag="QBF46_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDQ6673417.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGLSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            41786..43378
                     /gene="malX"
                     /locus_tag="QBF46_001209"
     CDS             41786..43378
                     /gene="malX"
                     /locus_tag="QBF46_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDQ6673418.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLNC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            43388..44560
                     /gene="malY"
                     /locus_tag="QBF46_001210"
     CDS             43388..44560
                     /gene="malY"
                     /locus_tag="QBF46_001210"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDQ6673419.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            44664..45665
                     /gene="add"
                     /locus_tag="QBF46_001211"
     CDS             44664..45665
                     /gene="add"
                     /locus_tag="QBF46_001211"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDQ6673420.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(45700..46740)
                     /locus_tag="QBF46_001212"
     CDS             complement(45700..46740)
                     /locus_tag="QBF46_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDQ6673421.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            46983..47108
                     /gene="blr"
                     /locus_tag="QBF46_001213"
     CDS             46983..47108
                     /gene="blr"
                     /locus_tag="QBF46_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDQ6673422.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            47381..47596
                     /gene="ydgT"
                     /locus_tag="QBF46_001214"
     CDS             47381..47596
                     /gene="ydgT"
                     /locus_tag="QBF46_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDQ6673423.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            47682..48122
                     /locus_tag="QBF46_001215"
     CDS             47682..48122
                     /locus_tag="QBF46_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDQ6673424.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            48199..48780
                     /gene="rsxA"
                     /locus_tag="QBF46_001216"
     CDS             48199..48780
                     /gene="rsxA"
                     /locus_tag="QBF46_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDQ6673425.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            48780..49358
                     /gene="rsxB"
                     /locus_tag="QBF46_001217"
     CDS             48780..49358
                     /gene="rsxB"
                     /locus_tag="QBF46_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDQ6673426.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSELCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            49351..>51130
                     /gene="rsxC"
                     /locus_tag="QBF46_001218"
     CDS             49351..>51130
                     /gene="rsxC"
                     /locus_tag="QBF46_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDQ6673427.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEA"
BASE COUNT        12494 a        13221 c        12587 g        12828 t
ORIGIN      
        1 ctgtgcggtc acatcctgtg aggaatatcg cgagtaagca tggtagtaat ttgtatttca
       61 caacagtcac cagagagtag agatgattct caatcatagt agcaaataca gtactttaca
      121 cgttaaatgc tatgcttaaa gaagttatct tcgcgtaagg agcttatgat gaaactctca
      181 acctgctgtg ccgcgcttct gctcgccctc gcctcacccg cggtactcgc cgcgccggga
      241 tcctgtgagc gcatacagag cgatatatca cagcgcatta tcaataatgg cgtacccgaa
      301 agcagcttca cgttaagtat tgtacccaat gaccaggttg atcagcctga ttcccaggtc
      361 gtcggccatt gcgctaatga tacgcataaa attctctata cccgcaccac cagcggtaac
      421 gtctctgctc ccgcgcagtc tagccaggat ggcgcgcctg ccgaaccgca gtaacacatt
      481 atcgactgaa cgccggatat gacaaatccg gcgatttgaa catacaacat aatcccacct
      541 tattactcat acccttctat tgatatggat taataattct taacccaaaa tgggtagact
      601 ccctctattg ttagcgcgct aaatattcaa tatataaata tttatataac gataaagaac
      661 agggagtgag ttatgtccaa aaatgaccga atggtgggca tcagccgcag aacactcgtt
      721 aaatcaaccg cgataggttc tctggcgctg gctgcaggcg gtttttcttt accgttcact
      781 ctgcgcagtg cagcggcagc ggtacaacag gccagcgaaa aagtgatctg gggtgcctgt
      841 tccgtcaact gtggtagccg ctgtgcactt cgtctacatg ttaaagataa tgaagtgacc
      901 tgggtggaaa ctgacaatac cggcagcgat gagtacggca accatcaggt acgcgcctgt
      961 ttgcgcggtc gctccatccg ctggcgtatt aatcatcccg atcgcttgaa ttacccaatg
     1021 aaacgcgtgg gcaaacgcgg cgaaggcaaa ttcgaacgga ttagctggga tgaagccctg
     1081 gatactatcg ccagtagcct gaagaaaacc gtcgaacaat atggcaatga ggctgtatat
     1141 attcagtact cttcggggat cgttggcggc aatatgaccc gctcttcgcc atcagcctcg
     1201 gcggtcaaac gcctgatgaa ctgctacggc ggttcactca accagtatgg ctcctacagc
     1261 actgcgcaaa tttcctgtgc catgccctac acctacggca gtaatgatgg caacagcacc
     1321 acggatattg aaaacagcaa gctggtcgtg atgtttggta acaacccggc agaaacccgc
     1381 atgagcggtg gtggcatcac ttatcttctt gaaaaagcgc gcgagaaatc gaacgccaaa
     1441 atgattgtta tcgatccgcg atataccgat acggctgccg gtcgtgaaga tgaatggctt
     1501 cctattcgcc cgggcaccga tgccgcgctg gttgcgggga ttgcctgggt attgattaac
     1561 gaaaatctcg ttgatcaacc ttttctcgat aaatactgcg tcggctatga cgaaaatacc
     1621 ttacccgcag atgcacccaa aaatggtcac tataaagcct atattcttgg tgaaggagac
     1681 gataaaacag cgaaaacgcc gcagtgggct tcgcaaatta ccggtatccc ggtggaccgt
     1741 atcatcaaac tggcgcgtga aattggcaca gcaaaacccg cttatatctg ccagggctgg
     1801 gggccacaac gccaggcaaa cggcgagctg accgcacgcg ctattgctat gttgcctatt
     1861 ctgacgggca atgtcggcat cagcggcgga aatagtggcg cgcgtgaatc gacctatacc
     1921 attaccatag aacgcctgcc ggtgctggat aatccggtca aaacgtcaat ctcctgcttc
     1981 agctggacag atgctatcga tcatggcccg caaatgacgg caatccgcga cggcgtccgg
     2041 ggcaaagata aactggatgt gcccattaag ttcatctgga actacgcggg aaataccctg
     2101 gttaatcagc attctgacat caacaaaacg catgaaattc tgcaggacga atcgaaatgc
     2161 gaaatgattg tggtcatcga aaactttatg acctcatcgg caaaatatgc cgacattctg
     2221 ctgccagacc tgatgaccgt tgagcaggaa gatattattc ctaacgacta cgccggtaac
     2281 atgggatatc tcattttcct ccagcctgtc accagcgaaa aattcgaacg caaaccgatt
     2341 tactggatcc tgagtgaagt cgcgaaacgt cttggacctg acgtctatca aaagttcact
     2401 gaaggtcgca cgcaggaaca atggttacaa catctgtacg ccaaaatgct tgccaaagat
     2461 ccggcgttac cgtcttacga cgaactgaaa aaaatgggta tctataagcg taaagatccc
     2521 aatggtcatt ttgtcgccta caaagcattt cgtgacgacc ccgaggcaaa tccacttaaa
     2581 acaccttccg gtaagattga aatttattcc agcaagctgg cggaaattgc ccgtacctgg
     2641 gaactggaaa aagatgaagt gataagccca ctgccagttt atgcctaaac ctttgaaggc
     2701 tgggactccc ctgaacgtag tacctttcca ctgcaactgt ttggtttcca ttacaaatcc
     2761 cgtactcact cgacctacgg caatattgat ctcctgaagg ctgcctgccg tcaggaggtg
     2821 tggatcaacc ctatagacgc acagaaacgt gggattgcca acggcgatat ggtgcgggtg
     2881 tataaccatc gtggcgaagt tcggctacca gccaaagtga caccacgcat tctccctggg
     2941 gttagcgcta tgggccaggg agcctggcac gaggccaata tgtctggcga taaaatcgac
     3001 catggcggat gtgtgaatac gctaaccact ctgcgccctt caccactggc gaagggaaac
     3061 ccgcagcaca cgaatctggt cgagatcgaa aaaatataac ccactacaac cataaattcc
     3121 ggcatgacat tttgtttgaa aagcaataag tgagtaatga tgaaaatcca taccacagag
     3181 gcgctcatga aggctgaaat cagccgtaga aatctgatga aaacctccgc acttggcagt
     3241 cttgcgctgg caagcagtgc tttcactctg ccattttccc aaatggtccg agcggcagag
     3301 acaccggtag aagagaaagc ggtctggagt tcctgcaccg ttaactgcgg gagccgctgt
     3361 ctgttacgtt tgcatgtgaa agatgacacc gtgtactggg tggagtctga tacgacaggt
     3421 gacgacgtct acggtaatca tcaggttcga gcgtgtttac gcgggcgctc tatccgccga
     3481 cggatgaatc atcctgatag gttgaaatat cccatgaagc gcgtcggcaa gcgcggtgaa
     3541 ggtaaatttg aacggataag ttgggacgaa gccctggata ccatcagtga taatcttcgg
     3601 cggatcctga aagattacgg caatgaggct gtacatgtcc tgtacggaac aggcgtagat
     3661 ggcggaaaca tcaccaactc aaacgtcccg taccgtctga tgaactcatg tggtggtttt
     3721 ctcagtcgct atggcagcta cagtaccgca cagatcagtg ccgcaatgag ttatatgttc
     3781 ggtgccaatg atggcaacag cccggatgat atcgccaata cgaaactggt cgttatgttc
     3841 ggaaataacc cggcagaaac acggatgagc ggtggtggtg tcacttacta cgtcgagcaa
     3901 gcccgagaac gttcaaacgc acgcatgatc gtcatcgatc cacgttataa cgacacagct
     3961 gccgggcgtg aagatgaatg gctgcccatt cgccctggca ccgatggcgc actggcctgt
     4021 gcgattgcct gggtactgat tactgaaaac atggtcgatc agccatttct cgacaaatat
     4081 tgtgttggtt acgatgaaaa aacgctgccc gccaacgcac cacgtaacgc gcattataaa
     4141 gcctatattc tgggcgaagg gcctgatggc atagctaaaa cgccggaatg ggcagcaaaa
     4201 atcatcagca tcccggcaga aaaaattatc cagttggcac gagagatcgg ttcagcaaaa
     4261 cctgcttata tttgtcaggg ttgggggcca caacgacact ccaacggcga acaaacatcc
     4321 cgcgctattg ccatgctttc cgttctcacc ggtaacgtcg gcataaacgg cggcaactca
     4381 ggcgtacgcg aaggtagctg ggatctgggg gtagaatggg tcccgatgct cgagaatcct
     4441 gttaaaacgc agatttccgt ctttacatgg acagatgcca tcgaccatgg tacggaaatg
     4501 accgcgaccc gtgatggtgt tcgtggaaaa gaaaaactgg atgtccccat caagttttta
     4561 tggtgctatg ccagtaacac attgatcaat caacatggcg acatcaatca cacccatgag
     4621 gtgcttcagg atgacagcaa gtgcgagatg attgttggca ttgaccactt catgacggcc
     4681 tcggctaagt attgcgatat cctgttgccc gacctgatgc caacagagca agaagacctt
     4741 atctctcatg aatctgcagg gaatatgggc tatgtgatcc tcgcccaacc cgcaacctca
     4801 gcaaaatttg aacgcaaacc catctactgg atgctgagtg aagtcgccaa acgcttagga
     4861 ccagacgttt atcaaacctt tactgaaggt cgcagtcagc atgaatggat caaatatctc
     4921 catgcgaaaa cgaaggaacg taaccctgag atgcccgact acgaggagat gaaaacgacc
     4981 gggatcttta agaaaaaatg cccggaagaa cactacgtcg ctttccgcgc attccgtgaa
     5041 gatccacagg caaacccgtt gaaaacacct tcggggaaaa tcgaaattta ttctgaacga
     5101 ctggcgaaga ttgcagatac ctgggaattg aaaaaagatg aaattattca tccccttcct
     5161 gcgtataccc caggttttga tggctgggac gatcccctgc ggaaaaccta tccactgcag
     5221 ttaacgggct tccattacaa agcgcgtacc cactccagct acggcaatat tgatgtgtta
     5281 cagcaggcct gcccacaaga ggtgtggatc aaccccattg atgctcaggc acgcggtatc
     5341 cgtcatggcg ataccgtgcg ggtatttaac aataatggag aaatgctgat tgccgcaaaa
     5401 gtgactccgc gtattctgcc tggcgttacc gccatcgggc aaggtgcgtg gcttaaagcg
     5461 gatatgtttg gtgaccgggt cgatcacggc ggcagtatca atattctgac ctctcaccgc
     5521 ccttcaccgc tggcaaaggg aaacccgtcg cacagcaatc ttgtccagat cgaaaaggtt
     5581 taaggagtag cccatgacca cacaatatgg attttttatc gattccagcc gctgtactgg
     5641 ctgtaaaacc tgcgaactgg cgtgcaaaga ttttaaagat cttggcccgg aagtcagttt
     5701 ccgccgtatt tatgaatacg ctggcggcga ctggcaggaa gataatggcg tctggcacca
     5761 gaacgtgttc gcttattatc tctccatctc ctgtaaccat tgcgatgacc ccgcatgtac
     5821 aaaagtctgc cccagcggcg caatgcataa gcgtgaagat ggctttgtgg tggttgatga
     5881 agatgtttgt atcggctgcc gctactgcca catggcttgt ccgtacggcg cgccacagta
     5941 caatgctgaa aaagggcaca tgacgaagtg cgatggttgt tattcgcgcg tcgccgaggg
     6001 gaaacaaccc atatgtgtcg aatcctgccc gctgcgtgca cttgagttcg gtccaattga
     6061 agagctgcgc cagaagcacg gcactcttgc tgccgtcgcg ccactgccgc gcgcgcattt
     6121 cacaaaaccc aatatcgtta tcaaacctaa cgccaacagc cgcccgaccg gcgataccac
     6181 tggttatctg gctaatccgg aggaagtgta aaatgggaaa tggatggcat gagtggccac
     6241 tggttatctt taccgtattg ggtcaatgcg tagtaggcgc gctgattgtt agcggaattg
     6301 gctggttcgc cgccaaaaat gacgcagacc gtcagcgtat tgtccgtggc atgtttttcc
     6361 tctggctgtt gatgggcgtc gggtttattg cctctgtcat gcacctggga tcgccactgc
     6421 gggcgtttaa ctcgctgaat cgaatcggag cttctggtct gagtaacgag attgctgccg
     6481 gttccatatt ttttgccgtt ggcggattat ggtggctggt ggcagtcatc ggtaaaatgc
     6541 cgcaagcgtt ggggaaactc tggttacttt tcagcatggc gctcggcgtc attttcgtct
     6601 ggatgatgac ctgcgtgtat caaatcgaca cagtgccaac ctggcataac ggttacacta
     6661 cgctggcttt cttcctgaca gtattgttga gcggtccaat acttgctgcc acaattctgc
     6721 gggcagcccg cgttacattt aataccacac catttgccat cattagcgtt ctggcgctga
     6781 tcgcttgtgc cggtgtgatt gtgctgcaag gtttgtctct ggcgtctatt cactcatccg
     6841 tacaacaagc cagcgcactg gtaccagatt acgcctcctt acaggtctgg cgtgtggtat
     6901 tgttatgcgc aggtctagga tgctggcttt gtccgctcat acgtcgccgt gaacctcacg
     6961 ttgccggact tattctgggg ttaatcctca ttcttggcgg tgagatgatc gggcgtgtgc
     7021 tcttttatgg cctgcacatg acggtaggaa tggctattgc aggttaacaa attggtgcgg
     7081 ggcaacccgc acatttcagg atgttaggaa tgacccattt ttcacagcaa gataattttt
     7141 ctgtcgcggc gcgcgtgttg ggcgcgctgt tttattacgc tccagagagt gctgaagccg
     7201 cacctctggt tgcggtactc accagtgacg gctgggaaac tcagtggcct ctaccagaag
     7261 cgtcattagc gccgctggtg acggcttttc agacacagtg tgaagagact cacgcccagg
     7321 cctggcagcg tttgtttgtc ggcccgtggg cactgccgtc tccgccgtgg gggtcggtct
     7381 ggctggatcg cgaatctgtg ctgtttggcg attcaatatt ggcacttcgt cagtggatgc
     7441 gcgagaaagg cattcagttt gaaatgaagc aaaacgaacc ggaagatcat tttggttcac
     7501 tgctgttgat ggctgcgtgg ttggcggaga atggtcgcca gacagaatgc gaagaattgc
     7561 tggcatggca cctttttccg tggtcaacac gttttcttga tgtttttatc gaaaaagcag
     7621 aacacccttt ctaccgtgca ctgggtgaac tggctcgctt aacgctggcg caatggcaat
     7681 cacaactgtt gattcctgtc gcggttaaac cactctttcg ataggatcac cgtaatattg
     7741 ccggatggtg acgattacgc gcgattcggc aatattacgt tacagtgtca cgttttttta
     7801 tctcttaaag cacgcactgc ttttgcggct ggcctctttt gccgcaaaat agtcgcccgt
     7861 gtttcattgc ccatttctgc tcatgcatca tctacacatc tatccggatc tgcgcactat
     7921 gttccaccgt ctgcttatcg caacagtcgt cggtattctc gcggcctttg ccgttgccgg
     7981 gtttcgtcat gcgatgctgc tactggagtg gttgttcctc aataatgact ccggcagtct
     8041 ggtcaatgca gcgacaaacc tttccccctg gcgacggctg ctaactccgg cgctcggcgg
     8101 actggcggcg ggtttgttgc tgatgggctg gcagaaattt acccagcaac gccctcatgc
     8161 gccgaccgat tatatggaag cgttgcaaac cgagggtcag tttgattacg cagcaagcct
     8221 ggttaaatcg cttgcctctc tgctggtagt aaccagcggc agtgcaattg gtcgcgaagg
     8281 tgcgatgatt cttttagctg cccttgccgc ctcctgtttt gcccaacgtt ttacgccacg
     8341 ccaggagtgg aaattatgga tcgcctgtgg ggccgcggcg ggaatggctg cggcctatcg
     8401 tgccccgctt gctggcagtt tatttatagc cgaagtgctg tttggcacta tgatgttggc
     8461 ctctctcggc ccggtgatta tttccgccgt cgtggcattg ctggttagca atctgattaa
     8521 tcatagcgac gcgttactct acaacgtaca actctcagtg acggttcagg ctcgtgacta
     8581 tgcgctgatt atcagtacag gtgtgctggc aggtctgtgc ggaccactgt tgttaacgtt
     8641 aatgaacgcc tgtcatcgtg gatttgtgag tctcaaactt gcgccgccct ggcaactggc
     8701 actaggcggg ttgatcgtgg gtctgctttc cctgttcaca cctgcagtgt ggggcaacgg
     8761 ctatagcacc gtacaatcct ttttaaccgc cccaccactg ttaatgatca ttgccgggat
     8821 cttcctttgt aaactgtgtg ccgtgctggc gagtagcggt tccggcgcac ccggtggggt
     8881 ctttacaccg acgctattta tcggtcttgc cattggcatg ttgtatggtc gtagcctggg
     8941 attatggttc cctgatggcg aagaaattac acttttactc ggattgaccg ggatggcgac
     9001 actgttggcg gcgaccacgc acgcgccgat tatgtcgacg ttgatgatat gtgaaatgac
     9061 cggggagtat cagctactcc ccggtttatt gattgcctgc gtaattgcgt cggtaatttc
     9121 gcggacgtta caccgtgact ctatctaccg ccagcacact gcgcagcata gctaagcgga
     9181 tgtattgccc cagttcacgc tgttcggcgc gcggcagata aggcagttca ccaatgagcg
     9241 gtgccggaag ttttttaccc agcacatcaa tgatttccgc ataatgcgcc agtcctgggt
     9301 tgattcggtt agccacccag ccaatgagcg gcagcccgtc gttggcgatc gcctgagctg
     9361 ttagcagtgc atggttaatg caaccttcct gaataccgac aaccatcaac accggcagtt
     9421 gttcctgcac tacccattca gagagtggac gcaaatcatt catcagactg cgccagccgc
     9481 cagtcccttc taccacgaca tgatcgactt tttcggtcag gtttgccagg ccgtttgaaa
     9541 tgagggtgta attgattggg caactgtgcg ccacgctact ttcttcttcg cttaacgcga
     9601 taggattaac tgcttcataa ggcagttcga tggttgaaac actctgcaac accagggcat
     9661 ctttattacg cagcccttcg ggtgtctctt tgctcccctt cgctacgggt ttatatcccg
     9721 caaccgtttt tccctgggag gctaacgctt gtagcaatgc gcgggaaacc accgttttcc
     9781 ctacagaagt gtctgtaccg gtaataaaga aacgcttcag catcactaac tccaccgtta
     9841 tgcttcacaa atataaacca ggaaaataat taaccttgaa agtctaagtt atgctttcct
     9901 ggcccaaatt gagatagcgc aaattttggt agaacagtta aaaaatgtta accctgcaac
     9961 agacgaatca acaaagaacc gttatacatc gcgtctttta ccagtgcagc gcctgccatc
    10021 gtgccctggt tagaaaactg agtactctca acgctgatgt gctgactata cgcaggaagg
    10081 gcctgctgac ggatgctgtc tgagatgacc gggaagagga tatctgccgc tttacttaac
    10141 ggtgagccaa tcagtatttt ttgtgggtta aataaattca ccatgatggc aagaatgcgc
    10201 ccgacatgcg cgcccacccc ggtaatgatg tcttttgcca gtagatcgcc gcgcaatgcc
    10261 gcctgacaca atgagtccac ggttaacggt tgcccatgta acatcgagct catggattga
    10321 ttgagacgca gctgtgccag ctcaagaata ctgtccacac tggcgatggt ttcgaggcag
    10381 ccgtggttcc cgcaataaca gcgtttacca tatgggtcga cctgtgtgtg gcctatttcc
    10441 acgagactac tgctgcctgc gtgtagcaga tgaccatcgg taatgacgcc cgcccccacg
    10501 ttgtgatcga taaccacctg aatcacatcg cgcgccccgc gtgaggcacc aaacaaggcc
    10561 tctgccatcg tccatgcgct gatatcatgc tgaatataaa ccggaacgcc ggtatgctgc
    10621 tccagagcct cgccgagcgg catctctttt acatcctcgt agaacggcat gcgatgtaca
    10681 atgccatttt ccgtatcaat aattcccggc agggttatgg caatcgaagt tagacgctca
    10741 agttttttct ggtggcggat aaaaaactga tcgatatggg aaatgatacg atccagcaat
    10801 ggcaagtcat cttttaacgc cagttcctgc gactcttcca ccaccagttt gctgctcaga
    10861 tcgcgcagag caaggaaaat ctccccgcga ctaatgcgca gagaaagata gtgccaggct
    10921 tcagtttcaa ccaccagccc caccgccgga cggccacggt tccccgcttc tttgatttcc
    10981 agctcttgca ccaggtgtgc ttcgagcatc tcacggacaa ttttagtgat actggcagga
    11041 gccagctgcg ccagacggga aagatcgata cgcgagactg gaccaagctg atcaatcagg
    11101 cgataaaccg cgcccgcgtt ggtctgcttt atttgatcaa tgtgcccagg ctggttttca
    11161 gcaaccaccg catactccct atattttcgc gctccgaaat aatctgtagg ctatggtgaa
    11221 gcacttcaat acgtgtcgtc aaatttttac ttaggcatgt gattaacagc acatttttcg
    11281 ggctttttcg ctgaaatttc cctcacctga gagcattcag cagatgaata cgaaagttac
    11341 gcgaagccgg actttgttca tgatgtttcg gccagaccaa ccacatttca gaaaccgcat
    11401 cctcttcagc aatcggcacc cagcgcattt cgttgagctg aacacgttta aatgacgcag
    11461 gcaaaattga aacacccaga ccggcggaaa ccagaccgat gatggtcatt gcctcgccca
    11521 cctcctgagt gatgacgggc gtcaaatggt aacgtcgcat cagtccgaga atatcgtcat
    11581 acaaccctgt cccgacgtgc ggatcaaaaa agacaaaggg ttctttcgcc agttcagcca
    11641 gtgttacact cggattattt gccaggggat gatcgtgcgg gatcatcgcc ataagcggtt
    11701 catggacgat gactgcgtgt tcaagcgtct ccggtaacgc tgtgttacgc agcaatccca
    11761 tatccagcgt tccttcaatg agcggagcga tttgctcacg agtgttcatt tcgcgggttt
    11821 gtaaatggac atcgggataa tcacggcgaa acagcgataa cgtatcggac accgcccgaa
    11881 taaaaggagc ggacgaagta aaaccaatgc gcaactcccc cgcttcaccc tgatgcagtc
    11941 tttcagcgcg agcggcagcg tcatctacca tagacaggat ttgccgacta tctgcaagaa
    12001 actgttttcc tgctgccgtc agcaatacac tgcgattggt tcgtgccagc agtcgggcac
    12061 caatttgttg ctccagcgcc tgaatctgct gacttagcgg cggttgcgaa atattcaggc
    12121 gggcagcggc gcgcccgaaa tgcagctctt ccgcaacagc aacaaagtaa cgcagatgac
    12181 gaagttcaat attcatattt aaaacatctt atttgagatt attaatatat tagacagaac
    12241 aattccattt tcctaatcta ttcataagcc tgatccacag gcatatttag caaggatttc
    12301 aagtgagccg tactacaact gttgatggcg ctccggcaag cgacactgac aagcaaagca
    12361 tttctcagcc aaatcaattt attaaacgcg gtacgccgca atttatgcgc gtcaccctgg
    12421 cgctgttctc tgccggactg gcaacatttg cacttctcta ttgtgtgcag cctatccttc
    12481 cggtgctttc gcaggagttt ggcttaacgc ccgcgaacag tagtatttca ctgtccattt
    12541 ccacggcgat gttggctatt ggtttgctgt ttactggccc cctatccgat gccattggtc
    12601 gcaaaccagt gatggtcacg gcgctactgt tggcctccat ttgtacgtta ctttcgacaa
    12661 tgatgaccag ctggcacggc attttgatta tgcgcgcctt gattgggctt tcgttaagtg
    12721 gcgtggcagc tgttggcatg acttatctta gcgaggaaat ccatcccagt ttcgtggcct
    12781 tttcaatggg gttgtatatc agcggcaact caattggcgg catgagcgga cgcttaatta
    12841 gcggtgtctt cacggacttt ttcaactggc gaattgctct ggcggcaatc ggttgtttcg
    12901 cgctggcctc ggcgttgatg ttctggaaaa tcctccctga atcacgccat tttcgcccga
    12961 cttcgctgcg ccctaagacg ttgtttatca actttcgtct gcactggcgt gaccggggat
    13021 taccgttatt gttcgcagaa ggctttttgc tgatggggtc gttcgtcacg ctgtttaatt
    13081 acatcggcta tcggttgatg ctctccccct ggcatgtcag tcaggccgtg gttggcttat
    13141 tatcgctagc ttatttgacc ggtacatgga gctcacccaa agccggaacc atgaccaccc
    13201 gctatgggcg tggtccagtg atgttgtttt cgacgggggt tatgctgttt ggtttactga
    13261 tgaccttatt cagctcgctg tggctgatct ttgccggaat gttactcttc tcagctggat
    13321 tcttcgcagc ccactcagtt gccagcagct ggatcggccc ccgcgcaaaa cgcgctaaag
    13381 gccaggcctc ctcgctgtat ctgttcagtt actatctggg gtcgagtatt gccgggacgc
    13441 tgggtggtgt tttctggcat aactatggct ggaacggcgt cggcgcattt attgctctga
    13501 tgctggtcat tgctctgctg gtcgggacgc gtttgcatcg tcgtctgcac gcctgaaaaa
    13561 taagtccgga ctgcggtaaa tacccgtccg gacttattgc ctgctcaaac caacgttaat
    13621 agccatccta aaatagacga agcgccagcc aattcccgca gcgcatctag cgtcataagg
    13681 attataagta cccaaataaa cggattcatt ttgctgtgtg tcatttatta ctgatgcgca
    13741 gttattctac cgctttgtaa gtagtaaaat agttaacccg ctcaagacta ctattattgg
    13801 tagctaaatt ttccttaagt cacaatacgt tattatcaac gctgtaattt attcagcgtt
    13861 tgtacatatc gttacacgct gaaaccaacc actcacggaa gtctgccatt cccagggata
    13921 tagttatttc aacggccccg cagtggggtt aaatgaaaaa acaaattgag ggtatgacaa
    13981 tgaaaaaagt attagctctg gttgttgccg ctgctatggg tctgtcttct gccgcctttg
    14041 ctgcagagac tgcgaccaca cctgctccga ctgcgacgac caccaaagca gcgccggcga
    14101 aaactacaca tcataaaaaa cagcataaag cagcacctgc ccagaaagcg caggcggcta
    14161 aaaagcatca taaaaatacg aaagctgaac agaaagcccc tgaacaaaaa gcgcaggcag
    14221 cgaagaaaca cgccaagaaa cacagccatc agcaaccggc aaaacctgct gcacaacccg
    14281 cagcgtaagt tttcaacagt aatgctggcg cgccccctcg cgcctgaaaa ttacggtgct
    14341 aagcgggtaa cgtttagcac cgcctttagc ggagggtagt atggtgggcc gttatcgctt
    14401 tgagttcatt cttatcattc ttattttatg cgcactgatt gccgcccgtt tttatctttc
    14461 ctgactgtag ttatctgatt ttactcccac tttcatcccg tcccgtctat agtatttatg
    14521 agggtttgct tttaataatc ataattaccc accagagtgt gatatgcgta caaccattgc
    14581 tgtagtgttg ggtgcaatta gtttgacgtc tgcttttgtg tttgcagata aaccagacgt
    14641 tgccaaatcg gcaaacgatg aggtcagcac cctgtttttt ggtcatgatg atcgtgtgcc
    14701 agtgaatgac acgacccaat caccgtggga tgcggttggg caactggaaa cggccagcgg
    14761 caatttatgt acggcgacgc tgattgcacc caatctggca ttaacggcag gacactgttt
    14821 attgacgccg cctaagggta aagcggataa ggcggtagca ctgcgttttg tgtcaaataa
    14881 aggtctttgg cgctatgaga tccacgacat agaaggccgc gttgatccga cactgggaaa
    14941 gcggttaaaa gcagatgggg atggttggat tgttcctccc gcagctgcac cgtgggactt
    15001 cggattgatt gtgctacgta atcccccttc tggcattaca ccgttgccgt tatttgaggg
    15061 agataaagcc gcgcttactg ccgcattaaa atcggctggt cgtaaagtga ctcaggcggg
    15121 ataccctgaa gatcatctcg atacgttata cagtcatcaa aactgtgaag tgactggctg
    15181 ggcgcaaacg tcggtgatgt cacaccagtg cgataccttg ccgggtgaca gcggttcgcc
    15241 tctgatgttg cataccaatg acggctggca attaattggg gtgcaaagtt cggctcctgc
    15301 cgcgaaagat cgctggcgcg ccgataaccg ggccatttct gttactggtt ttcgcgacaa
    15361 gctggatcaa ctgtcgcaaa aataatgttc aaacgctgcc cgacagcgcg ggcagcgtct
    15421 tcatcaggca agtttcacca tgatcattcc agccaacagc aagaccaggc caatccagcc
    15481 tttacgattt aaccgttgac caaacaagat ccaacctgcg gctaacgtgg cggcaatacc
    15541 aaacccgccc cacaatgcat aagcgacaga caagtcgatc cctttaacgg cctgagaaag
    15601 cgcactaaag gcagccagca ccgccgccag ggagagcaag ccaaatattt tgcgacgaaa
    15661 gccgtcagaa aatttcaaaa agacgttagc aacgatttcc agcacgattg ccaatgccag
    15721 ccaggcggcg tgaacccatt caaactgcgc catggttcac ctccagttca ggtttacgcg
    15781 ctttacgggt acctgatttg atcaacacaa tcccggcgac cagggtggtt aacccggcaa
    15841 ttttcatcag cgataaactt tcgtcgaata acaaaacgct aaacaaggta ataaataaaa
    15901 taccgatacc ttcccacagc gcataagcta cgcctaaggc gattttttta acggcgaaag
    15961 agagaaatat atacgacaga gaaatcatca ccagcattaa aataaagccg ccatttccct
    16021 cactgacgct cgcccatttc attgacagcg taccggtaat ttctgtagca atagccagac
    16081 ctaataaaat ccaataaata tacattgtcc ttctcctgca agagaattat tttaattttc
    16141 gcttaattca gcgaaaccaa agtaaagtgg tcaagccccg cgcatagcgc aagctttggc
    16201 agaagaaagg actaaagcgc gttgcgccag tgctgctcac ctacgagcaa gatagtggat
    16261 gaggtacgaa gatgggtaaa tgtagaaaat aacgtcctga acaaattgtc cataatatta
    16321 caattatccg cagtgttgct tctcgtcatc gcggatgata attgtcctcg gtagttgaac
    16381 acgcctgatt tgtatcatag ctcaagaatt aactcaaaat attttcactt ctttacctga
    16441 gctgtttgat tttcgttatg atgacggagc gaaaaagaca ttattattag caaaggaaga
    16501 aaaaacgggg acaagcatgg caaagccgat catcacgctc aatggcctaa aaatcgtcat
    16561 tatgttggga atgctggtca ttattctctg cggtatccgt tttgccgccg agatcatcgt
    16621 gccgtttatt ctcgcattat ttattgctgt tattcttaac ccgctggtgc aacacatggt
    16681 ccgctggcgt gtgccgcgtg tactggcggt gtcgattttg atgaccatca tcgtgatggc
    16741 gatggtgttg ctattagctt atctgggttc cgcgctcaac gagttgacgc ggacgttacc
    16801 gcaatatcgc aactctatta tgacgccgct gcaagcgctt gaaccgttgt tgcaacgcgt
    16861 agggattgac gtctcagttg accagctggc gcattatatt gatccgaacg cggcgatgac
    16921 gttgctcacc aacttattga cgcagttatc taatgccatg tcatcaatat ttttattgct
    16981 gctgacggtg ctgtttatgc tgctcgaagt gccacaattg cccggaaaat ttcagcaaat
    17041 gatggcgcgt ccggttgaag ggatggcggc gattcaacgt gcgattgaca gtgtttctca
    17101 ttatctggtg ctgaaaacag ccatcagcat catcaccggc ctggtcgcct gggcgatgct
    17161 cgccgcactc gatgttcgct tcgcttttgt ctggggattg ctggcctttg cgcttaatta
    17221 catcccgaat attggttcag tcctcgcggc aatcccccct atcgctcagg tactggtgtt
    17281 taatggcttc tacgaagcgt tgctggtgct ggcgggatat ctgctgatta atctggtctt
    17341 cggcaatatt ctggagccgc gtatcatggg gcgtgggctg gggctttcca cattggtggt
    17401 atttttgtcg ttgatttttt ggggatggtt gttaggaccg gtgggtatgc tgctttccgt
    17461 gccgttgaca attattgtca aaattgcgct tgaacaaaca gcgggaggtc aaagcatcgc
    17521 cgttctgtta agcgatctca ataaagagtg acggcctcag cagaggccgt cagggttaca
    17581 gagctttcag gattgcatcc acgctggctt tggcgtcacc aaacagcatg tgggtgtttt
    17641 ccttgaagaa cagcgggttt tgcacaccag catagccagt gttcatcgaa cgtttaaaga
    17701 caatcacgtt ctgcgctttc cacacttcca gcacaggcat accagcaatc ggactcttcg
    17761 gatcatcctg cgccgccggg ttaaccgtat cgttagcacc aatcaccagt acggtatcgg
    17821 tatcagcaaa gtcatcattg atctcgtcca tttccagcac gatgtcatac ggtacttttg
    17881 cttcagccag caatacgttc atatgtccag gcaaacgccc cgcgaccggg tggataccga
    17941 aacgcacatt aataccacga gcgcgcaatt tctcagtaat ttcagcgaca ggatattgcg
    18001 cctgcgcaac tgccatgccg taccccggag taatgatcac tgaatgggag tttttcagca
    18061 gttccgctgt ctcttctgca gtgatttcgc ggtgctcgcc cacttcctga tcatcgccag
    18121 tagaagagcc gtcggtgccg aaaccacccg caataacgct gataaaggaa cgattcatcg
    18181 ccttacacat aatgtaagaa aggatagccc ccgaagaacc gaccagcgca ccggtcacaa
    18241 tcagcaggtc gttgctgagc ataaagcccg cagccgcagc cgcccagccg gagtacgagt
    18301 tcagcatcga caccaccact ggcatatctg caccaccgat ggaggcgact aaatgccagc
    18361 cgaataccag cgcaatggcg gtcattatta gcaatgccag cacttgcagg ccgacgctgt
    18421 ccgtgcgaac aaatacaatc agcagcagga aggaaacgac cagagccgcc aggttcattt
    18481 tgtgacggtt tggcagcatc aacggtttag acgaaatctt gccacacagt ttgccgaacg
    18541 ccaccaccga acccgtgaac gttaccgccc cgatgaagat accgaggaac acttccgtca
    18601 ggtgaatatt gaccagaatc ggtgccattc ccgcgtcatg atgcagatag ctgttaaagc
    18661 caaccagcac tgccgccaga cccacgaagc tgtgcaggat cgccaccagt tctggcattt
    18721 cggtcatttc aactttcttc gccagacgga taccaattgc cccaccaatg accatcgcca
    18781 gcaagatcca gccaacatta cccgtatccg gtccaaaaat ggttgcgatt aacgcaatcg
    18841 ccatcccggc gataccgaag ttgttaccct ggcgagacgt ttcatgtttc gaaagaccgg
    18901 ccagactgaa gataaacagg atcgcggcaa caatgtatgc agctgtaact aatcctccag
    18961 acatatgtta ccccttaatt tttgcggaac attttcagca tgcgctgagt cacggtgaag
    19021 ccaccgaaaa tattaatgct ggctataagc accgcgataa aactaaggaa gctaacccag
    19081 ccgccctggc caatctgcaa cagtgctccg acaacaataa tccctgaaat cgcgttggtg
    19141 accgacatca acggtgtatg cagcgcgtgc gatacattcc acaccacgta ataaccgaca
    19201 acgcaggcca gcgcgaaaac ggtgaagtgc ccaagaaact ctttcggcgc aacgcttgcc
    19261 agccaaccga aaaggatgat tgccagcgcc atcaacgcgt atttacgcca cggtgagcag
    19321 gcacattttt cctcagtttt cacttccggt gccgcttttt gtgccgcctg cggctgagct
    19381 gatacctgaa tcggcggtgc cggccaggta atttcgcccg cacggatcac ggtcacgccg
    19441 cgaatcacca catcatcaaa atcaacagtg atattgccgt ctttctcttt gcacaacagt
    19501 ttcagcagat taacgaggtt agtaccgtaa agctgcgagg attgcgtcgg cagacggccc
    19561 ggaagatcgg tataaccaat cactttgaca ccattttccg tagtgaagat ttcacccggc
    19621 acggtgtatt cacagttgcc accgttttgg gctgccaggt cgacaatcac actgcccgcc
    19681 ttcatggagt caaccatttc acgggtaatt agcttcggcg ctggtttgcc tggaataagc
    19741 gcggtagtga caatgatatc gacctctttt gcctgggcgg caaagagttc catttccgct
    19801 ttgatgaacg cgtccgacat cactttggca tagccatcgc cgctgcccgc ttcctcttta
    19861 aaatccagct cgaggaattc cgcgcccata ctttgaactt gttctttcac ttccgggcgg
    19921 gtgtcgaatg cacgcacaat cgcgccgaga ctgtttgctg cgccaatggc ggccagacct
    19981 gcaacaccgg caccaatcac catcactttt gccggtggca ctttcccggc cgcagtgatt
    20041 tgcccggtaa agaagcgccc aaattcatgt gccgcttcaa caatggcgcg ataaccggcg
    20101 atattcgcca tcgagcttag tgcgtccagc gattgtgcgc gtgagatacg cggcacagag
    20161 tccatcgcca tcacggtcac gttacgttcc gcaagttttt gcattaattc cggattctgc
    20221 gcaggccaga taaaactcac cagcgttgtc ccaggattca gtaacgcaat ttcatcatct
    20281 aacggcgcat tgaccttcag aatgatctct gactgccaga cgctattccc ttctacaatt
    20341 tcagcgcccg cttgcacaaa cgctttatcg tcaaaacttg ccagttgacc cgcgccgctc
    20401 tctaccgcga cggtaaaacc cagtttcagc agctgttcca ctgtttttgg cgttgctgca
    20461 acacgggttt cattggttaa ccgttctctt ggtatgccaa ttcgcatgat attcccttcc
    20521 atcggtttta ttgatgatgg tttgcctgtg tcaggagcca cacaagctgc tcatgtacga
    20581 gctaaatgtt actccgttaa aataaattag taacaaacgc cctataacgt actgaaaatt
    20641 atgcctgtga tctagcgcca aaaatcagta tttcggcgtg aactcgcaaa atattaacga
    20701 ttcagccgtg atagtgggat aaacacctta gaacgccgga taaagactga taattgtctt
    20761 cgacggtcgg gtaaaacgag acatcgcccc ggcacgaatc actacttaac attaaattaa
    20821 cttatacaat tcagttgctt cagtagtaat gatgctgata cggctgtttt ttaagcatag
    20881 acggtcattt gagcaggatt aaaattggct taaggaatgt gatatgaaaa atgacgcaga
    20941 cagttacacc gtttaaatgc aataatcagc cacgtttctc gttaataaca ataccagtac
    21001 ctggtttgcg caaggcgaag gattattttt atgaagctta agaacaccct cctggcgtcg
    21061 gcactgcttt ctgctacggc attctccgtt aacgcagcaa cagaactgac accggagcaa
    21121 gcggcagcgg ttaaaccttt tgaccgtgta gtggttaccg gtcgttttaa tgctattggc
    21181 gaagcggtga aagccgtttc tcgtcgcgca gataaagaag gtgccgcctc tttttatgtt
    21241 gtcgacactt ctgattttgg taacagcggt aactggcgtg tggtcgctga cctctataaa
    21301 gccgatgctg aaaaagcaga agaaacaagt aatcgcgtaa ttaacggtgt tgtcgaactg
    21361 ccgaaagatc aggctgttct gattgaaccg tttgacacgg tcaccgtcca gggcttctat
    21421 cgtagccagc cagaagtcaa tgatgccatc accaaagcgg caaaagcgaa aggtgcctac
    21481 tctttctaca tcgttcgtca aatcgatgcc aaccagggcg gcaaccagcg tattactgca
    21541 ttcatctata aaaaagatgc taagaaacgt atcgtccaga gcccggatgt gatcccggca
    21601 gattccgaag caggacgtgc agctctggct gccggtggcg aagccgcgaa gaaagttgag
    21661 atcccaggtg ttgcgactac cgcatcacca agttctgaag tcggtcgttt ctttgaaacc
    21721 cagtcatcaa aaggcgggcg ttacaccgtc acgctcccgg atggcactaa agtcgaagaa
    21781 ctgaacaaag cgaccgcagc gatgatggtc ccgttcgaca gcatcaaatt ctctggcaac
    21841 tacggcaaca tgaccgaagt ctcctatcag gttgcgaaac gtgccgcgaa gaaaggtgct
    21901 aagtattacc acatcacccg ccagtggcag gaacgtggta ataacctgac cgtcagcgca
    21961 gatctgtata aataactatt cagttactcc tataaggcgg cttgatgccg cctttcgcat
    22021 ttttagtcac aaatttcccc gcgaccattg catgcagaca catctcttcg taaaatcccg
    22081 agcttcagtg gcaaactcca tttttatgcg ttttgcccta atatttattc ttcttatcac
    22141 gttttaatca ctggatatcg atggaaaaga aactgggact gagcgcactc accgcgctgg
    22201 tattaagctc aatgctgggc gcgggtgttt tcagtctgcc gcaaaatatg gcggcagttg
    22261 ccagcccggc agcactgctc atcggctggg gtattactgg cgctggcatt ttattgctgg
    22321 cctttgccat gctgatcctc acgcgcattc gtcccgaact tgacggcggt atctttacct
    22381 atgcccgcga agggtttggc gagctaatcg gcttttgttc cgcatgggga tactggctgt
    22441 gcgcagtcat cgccaacgtc tcctatctgg tgatcgtttt ttccgcgtta agctttttta
    22501 cggacacgcc ggaattgcgc ctgttcggcg atggtaatac ctggcaatcg atagtcggtg
    22561 catcggcttt attgtggatt gttcactttt tgatcctgcg cggggtgcaa accgctgcca
    22621 gcattaacct ggtggcgaca ctggcaaaat tgttgccgct gggtctgttt gttgtgctgg
    22681 cgatgatgat gttcaaactg gataccttca agctcgactt caccggactt gcacttggcg
    22741 tacccgtttg ggaacaggtg aaaaacacca tgctgatcac cctgtgggtt ttcattggtg
    22801 tggaaggtgc cgtggttgtt tctgcgcgtg cgcgtaataa acgtgatgtt ggcaaagcga
    22861 cactgctggc ggttctctcc gctctgggcg tttacttgct ggtaacgctg ctttcgctgg
    22921 gcgtggtggc acgtcctgaa ctggctgaaa ttcgtaaccc gtcaatggcc ggtctgatgg
    22981 tggaaatgat gggaccatgg ggcgaaatca tcatcgctgc cggtttgatc gtttccgttt
    23041 gcggcgcgta cttaagctgg accatcatgg cggcagaagt tccgttcctg gctgccactc
    23101 ataaagcatt cccacgcatt tttgcgcgcc agaacgcaca agctgcgcca tccgcctcgc
    23161 tgtggttgac caacatctgc gtgcaaattt gtctggtact catctggctg accggttccg
    23221 attacaacac gttgctgacc atcgcctcag aaatgattct ggtgccctat ttcctggtcg
    23281 gcgcgttctt gctgaaaatc gcaacccgtc cactgcataa agcggtaggt gtcggtgcct
    23341 gcatttatgg cttatggtta ttgtatgcct ctggtccaat gcacttgctg ttatccgttg
    23401 ttctgtatgc gccggggctg ctggttttcc tgtatgcgcg caaaacgcat acacatgata
    23461 atgtactgaa ccgtcaggaa atggtgctga ttggcatgct gttaattgcc tcagttccgg
    23521 cgacctggat gctggtggga taacgttcat cccatcgttg gatagcaaag gagataacga
    23581 tgggtaaagc ccagcccttg ccaatattaa ttactggcgg aggtcgtcgc atcggcctcg
    23641 ccctcgcctg gcatttcatt aatcaaaagc aaccggtgat tgtcagctat cggacgcact
    23701 atccagccat tgatggactg attaaggcag gtgcgcagtg tattcaggct gatttttcga
    23761 ccaacgacgg tgtgatggcg tttgccgatg aagtactaaa aagcacccat ggtctgcgtg
    23821 ctattttgca taacgccagt gtgtggatgg cggaaaaacc ggatgcgcca ctggccgacg
    23881 tactggcttg catgatgcag atccacgtta ataccccata cctgctcaac catgcgctgg
    23941 aaagattact gcgtgggcac ggacacgccg ccagcgatat cattcacttt accgattatg
    24001 tggtggagcg cggtagcgac aaacatgttg cgtatgctgc aagcaaagcg gcactggata
    24061 atatgacccg ctcgtttgcc cgcaagctgg caccggaagt gaaagtgaat tctattgcgc
    24121 catcgctgat cctgtttaat gaacatgatg atgccgaata tcgacaacag gcgctgaata
    24181 aatcactgat gaaaaccgcg cctggcgaga aagaagtgat cgacctggtc gattacttac
    24241 ttaccagttg ctttgtcacc ggacgcagtt tcccacttga tggcggtcgt catctgcgtt
    24301 aatgcagctt tatccagcat ataatcaaca cccatgcgct tatcccccaa cacgcaacag
    24361 aaccaacaaa agcggcgggc aagcgcatca ttccggtgaa ataccacagc gacaccaggt
    24421 aaacaaaata gggaataatc gaccacatac taaaaatgat ggttgcgcgt aaggcttcaa
    24481 tgccgcgttc gctggcaaca atataatgcg cgataagcgc aaaggtcggg aaaagtggaa
    24541 tcagcccggc gatataataa tttttcgttt ttgctaaaac accaatcaac agcactacca
    24601 gcgcaccgag cgcggctttg attaccagcc ccatcttttt accttaacac ttccataaca
    24661 agtcatcagt agaatacctg atgaaaactt gtttagaaac gattgatagt aagtaaaaac
    24721 agcgcggtgt attgtgacgt ttttatatct accgtgaatg ttatgaacac tatcgtattt
    24781 gtggaagatg atgcggaagt cggttcactg attgccgcgt acctggcaaa acatgatatg
    24841 caggttaccg tagaaccgcg cggcgaccag gccgaagaaa ccattttgcg agaaagtccg
    24901 gatttggtgt tactcgacat catgctacca ggcaaggacg gcatgaccat ttgtcgtgat
    24961 ttacgcgcaa agtggtctgg accgattgtt cttctaacct ctctcgatag cgatatgaac
    25021 cacatcctgg cactggaaat gggtgcctgc gactatattc tcaaaacgac gccccctgct
    25081 gttttgctag cgcgtttacg tttgcatttg cgtcagaatg agcaagccac actgaccaaa
    25141 ggtcttcagg aaacgtctct gactccctac aaagccctgc atttcggcac gttgaccatc
    25201 gatcccatca accgcgtagt caccctggct aacactgaaa tctcgctctc gacagctgat
    25261 ttcgaattat tgtgggaatt agctacccat gccgggcaaa tcatggaccg cgatgcattg
    25321 ctgaaaaatt tacgcggcgt cagttatgac ggactggatc gtagcgtgga cgtggctatt
    25381 tcgcggttaa gaaaaaaact gctcgataac gccgcagaac cttatcgcat taaaactgtg
    25441 cgtaacaaag gctatctttt tgcgcctcat gcatgggaat aagcgatgaa aaaactgttt
    25501 atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct ggttgggttg
    25561 gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt gatgaacagt
    25621 tcgctgtatc tgatgcgcag cgaattgcgt gagatccccc cacacgactg gggtaagacg
    25681 ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact gagtaaatac
    25741 catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc cctggacgat
    25801 cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt tggtcctgtt
    25861 ccttatcttt attatctcca tcagatgcga ttgctggata tcgccctgat cgcttttatt
    25921 gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca ggatatgtta
    25981 aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg tatccacttt
    26041 gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc ggacaatatc
    26101 aacgccttga ttgccagcaa aaaacagctt attgacggta tcgctcacga actgcgaaca
    26161 ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc cgccgaatcc
    26221 caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct gctgacttat
    26281 gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct gccgttgtgg
    26341 ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt acggattaaa
    26401 acgctcgcgc aaggccatta tgcggcgttg gatatgcgct taatggagcg cgtgctggat
    26461 aatttgctca ataacgccct gcggtactgc cattcaacgg ttgaaaccag cctgctactg
    26521 tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc cccagaaaac
    26581 cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg ctcaaccggc
    26641 ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg cggtacggtt
    26701 aattgtgaca ccagcgaact gggtggtgct cgcttctcgt ttagctggcc gttatggcat
    26761 aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt caccaccact
    26821 gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg ttacgatctc
    26881 gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctgc atttgccgct
    26941 catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga ggtaaaaaaa
    27001 gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg caacgacgcg
    27061 cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc cgaaaatcgc
    27121 agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga taacctttcg
    27181 caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac gttcgagcat
    27241 atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt gcatcgtcat
    27301 ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca cgaccccgcc
    27361 actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg tgatgaagtc
    27421 ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg gacgcgcgag
    27481 gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc acagaacgcg
    27541 aagttaaaaa tcaaacgtcc ggtgaaggtg cagccgattg cccgcgtctg gtacaaagga
    27601 gatcaaaaac aagtgcaaca cgcctgccct acaccgctga ttgcactgat taatcgggat
    27661 aatggtgcgg gcgtgccaga cgttggtgag ttgttaaatt acgatgccga caatgtgcag
    27721 caccgttata aaccacaggc gcagccgctt cgtttgatca ttccacggct gcacctgtat
    27781 gttgcagatt aacgcccggc tttcatactg ccgaccatct gttctggccg tacccagctg
    27841 tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa ggtcagccct
    27901 tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg gttgttaagc
    27961 gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg attcggttca
    28021 atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa gcgcaccgat
    28081 tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt accggaagcg
    28141 ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat ggttaatgct
    28201 tcacactggg ttgggttcac tttacccggc atgattgagc tgcccggctc attttccggg
    28261 attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac atcattggcg
    28321 attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac cagggcatca
    28381 caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca ggtaatgact
    28441 gccagttcat ctgctacgcg acgcgcatac tccggatggg tatttagtcc agtacccacc
    28501 gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat atgtttgaga
    28561 ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag cggcgtggcg
    28621 tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga tttttcactc
    28681 agtgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagcgc cagcagcgcc
    28741 gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt cacgtcgtcg
    28801 ttagggtgaa ctttacgttc catcccgcgc acgccgccga gtaattcact gccccggtta
    28861 gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg ccagatagcc
    28921 agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg ccgaatggcg
    28981 ctcgctttct cttcagacaa caagcctaaa tcttcattaa cttttgccgc tgcgcgcttg
    29041 gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat gcggaaatgc
    29101 tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc aatcgccccc
    29161 atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctgt ttaatttttt
    29221 ctttctgttt tgctttcgtt aagcaacttt taggtcactt attatttact atttgataac
    29281 aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct ctgtttattt
    29341 cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc ctttatcatc
    29401 cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt ccattcccag
    29461 ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata cagcggccgg
    29521 accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg tcacctgctg
    29581 gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca gttgatcgac
    29641 ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat aaccttccgg
    29701 cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtactgcg gcagcccttc
    29761 accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccga cgataatcgt
    29821 gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga ggatctcttt
    29881 catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac gcagctcttc
    29941 agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct gacggttgat
    30001 cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg gacaggatgc
    30061 gccgtgacgt ggcaggcgaa tcacgcgtat gtcgtgagcg aagtatttac caccaaactg
    30121 cgcacccaga ccgagatttt gcgcttcgat cagcaattct ttttccagtt ccacatcgcg
    30181 gaatgcctga ccgtgctcat tcccttccgt tggcagttcg tcatagtatt tagcggaagc
    30241 cagtttgacc gttttaaggt tcgtttctgc agaagttcca ccaataacga acgcaatatg
    30301 atacggagga caggccgccg tacccagcgt gcgcatcttc tcaaccaggt aatttttcag
    30361 tttccccggc gttagtaacg ctttggtttc ctgatagaga tacgtcttgt ttgccgaacc
    30421 accacctttg gcgatacaga ggaatttgta ctcgtcgcca tcaacggcat aaagatcgat
    30481 ctgcgctggc agattggtgc cggtattcac ctctttatac atatccagcg gcgcgttttg
    30541 cgagtagcgc aaattatctt cgatataagt gttatagaca ccgcgcgcca gcgccgcttc
    30601 atcacagcca ccggtccata cacgctgccc ttttttacca acaataatcg cagtgccggt
    30661 atcctgacag gttggcagaa cacctttcgc cgcgatgtcg gagttgcgca ggaattgcag
    30721 cgccacatat ttatcatttt cgctggcttc cgggtcacgc agaatgtcgg ccacctgttg
    30781 ttggtgagcc ggacgcagca taaatgacgc atcatgaaac gcctgacgcg ccaacagagt
    30841 taacgcttcg ggtgcgactt tcaaaatctc ctgccattca aattcagata cgctaacgtg
    30901 ttcgctggtt agcaggtaat actcagtatc atcttttttg agtggaaaag gagcctgata
    30961 atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt atgctctggg
    31021 cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacactt ttttaacaaa
    31081 aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat tcttgcttta
    31141 atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact cattaactca
    31201 gtgcaaaact atgcctgggg cagcaaaacg gcgttgactg aactttatgg tatggaaaat
    31261 ccgtccagcc agccgatggc cgagctatgg atgggcgcac atccgaaaag cagttcacga
    31321 gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag tgataaatcg
    31381 actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct gttcaaagta
    31441 ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa ttctgaaatc
    31501 ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg taactataaa
    31561 gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc gatgaacgcg
    31621 tttcgtgaat tttccgagat tgtctcccta ctccagccag tcgcaggtgc acatccggca
    31681 attgctcact ttttacaaca gcctaatgcc gaacgtttaa gcgaactttt cgccagcctg
    31741 ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc ggcgctcgac
    31801 agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta cccggaagac
    31861 agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg cgaagcgatg
    31921 ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga agtgatggcg
    31981 aactccgata acgtgctgcg tgcgggtctg acgcctaagt acattgatat tccggaactg
    32041 gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca gccggtgaaa
    32101 caaggtgcag aactggactt cccgattcca gtggatgatt ttgccttctc gctgcatgac
    32161 cttagtgata aagaaaccac cattagccag cagagtgccg ccattttgtt ctgcgtcgaa
    32221 ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg tgaatcagcg
    32281 tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt agcgcgtgtt
    32341 tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc ttattaaagg
    32401 cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa tcattatgaa
    32461 taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga caggcggcgc
    32521 atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc aggcgaacgc
    32581 gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa actatcatcg
    32641 cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga aagaaaatcc
    32701 gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg gtcccttccc
    32761 gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc aaaccacgct
    32821 ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa cgccttttga
    32881 gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc tcaagccact
    32941 gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc agttaaatgc
    33001 tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc ggatagacgc
    33061 agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa ccgacggttc
    33121 cagcacgctg gcaagttttg gtgagcgtgt aggaaatcaa aaactgtcac tggaaaaaat
    33181 gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga tcagcggtaa
    33241 atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact cgctaaacag
    33301 cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag tcggccagat
    33361 tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc aggcgctgct
    33421 ggcacagcca gaaattgcca acaaccccga actttatcag gagaaagtga cggaagcctt
    33481 ctttagcgcc ctgccgctga tgctgaaagg cgatccggtg attactatcg cgccgctaag
    33541 ctggaaaaac agtcagggtg aaagtgccct gaatctgtcg ctgttcctga aagatccggc
    33601 aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg ttaaatctct
    33661 ggatgcgaaa ctgaccattc cggtggatat ggcaactgag tttatgactc aggtagcgaa
    33721 gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag ttgaaggtgc
    33781 atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca tcaccaccag
    33841 cctgcaatat actaacggtc agataacgtt aaacgggcag aaaatgccac tggaagattt
    33901 cgttggtatg tttgcaatgc cggcattaaa tgttccggtc gtacccgcta ttccgcagca
    33961 gtaattcaca gccaaaacgc cggagatttt tctctccggc gttatttctt acttcagcat
    34021 aaagtcatac tgaatactca ttttcacatc ccagtaattc aggcgatcgc ccagcgtact
    34081 gccatcgcgc ttttcccgcc ctttatcggc agcaagaaag aaattcatcc cttgtaacgg
    34141 cccttcgctg acccgatagt tcataccacc taagataccc gtgccttcaa tccgcacatc
    34201 tcgtttactg gattcatagc cgcgagtcac aatcggtgca aatgtcagtg taaattgcgg
    34261 cgttaaacga tagttgacgc ccaattgcca ggactgcatt ccttcacggt tacgatcaag
    34321 acttaatgaa aagggatagc caatgtcggt atccaccacg gcaccattca ttgcatggcg
    34381 caaataacca ccactgccaa atacgcttcc ccacggcgca ttccaggtca attgtccact
    34441 aaccaacgcc gtttcattgg gctggctggt attacgactt aaaccttcga gctgcgcgta
    34501 aaagccgagc aactcccctt tcaacgtata attcgggtcg agcggtatgc ttttctcgag
    34561 aatactttgt atctgattgg aaacatcttt ctgtaccgat gcccccagag aatagcgact
    34621 ggtatcatca tgccaactgg cggctaaaaa agaacgtgcg cgatgtttat cgtattttcc
    34681 cgtctggctg ttatacaccc cttcatcacg aataccggtt gttgaatcag tccataaaaa
    34741 tactcgtgaa cgccacattc cccacagatc aatatttggt gttaaagccc aataacctaa
    34801 tgctccttca gaaactgtcg gtaatgtacg ttgcgaactg ccagccatta acggaatacg
    34861 ataaggtaag ctaccataac tataatccgt cccgaagcga cctaatttaa gcaggctatt
    34921 ttcactgggt tttattttta ctgcccccag ggcaaagcca aaacttttat caccatcaag
    34981 ataccaccgg gtactcatat cagcacgagc acctaattta taaacataat acgccccgcc
    35041 ttcaacgcca ataatattgg aataataacc actattgaaa tcgagcaatc ctccctgtac
    35101 ccatgcgtaa atatctcttc ccgcgccagc actgggctta tccgccctgc gcaattcgtt
    35161 tttcagtctg atgcgtagtc ccgcttcatc gtcagccagt tgcgccgcat aagcagaggt
    35221 aaggccagag gcagccgtca ggcaaataac ggccatggcc actatttttc tcatagttgc
    35281 acctttgatg ttctgattat ttttattgaa tattaattag tgatatcgct gattaattgc
    35341 tgctgcactt ttttacgatt atcaatttca accacgattt ctttgaattt tttatccgtg
    35401 agcggataaa accagataat aacgaatgcc agtagcataa atccgcaagg tactaaggca
    35461 attgatgtgc ggatgcccat aataacttcc ggcgtttgca cctgattggc gatatatccg
    35521 cttaacccca aaataaaggc aggaattgaa cctccgattg cctgaccgca tttacgggta
    35581 aatgagaata gtgaataggt gagcccttca attcgcacgc cggtcaggta ttcaccgtat
    35641 tctacggtat cagcttccag cgcccacatc acggtcatgg taacgccctg accaattgaa
    35701 gcgatggcca acgcaaccaa cgccaccggc agtgaccaga cggaaaccca gaagaacagc
    35761 agataaccgc aggttcccag caaagcgcca atcaggaagg tattcttttt accgatcctc
    35821 gcgaccatcc ccggcaccag cggtgccgat gccacagtac caaccaggtt ttgcaccagt
    35881 accagcacag tgaacagccc ggtatcattt aacacatagc gcacgtagaa caacgacgag
    35941 gcgctgaccg caaaggtcga aatcagcaca cacagcgcac cgatgcacaa cataaacagc
    36001 gggcgattcc gtttcagggt ttgcagactg atattcaatg acggctgcgc aacgatacgt
    36061 accacattct cacgcgtcga tttgaagcag atgaagtaaa gcaccattcc ggcaatcgcc
    36121 agcacaattg tccagaaatg gtataccgac accatctctt ccgggctgga gttcttaatg
    36181 ctcggtccta tcagaaatgc caggcagaca aaggtcaatg aagcggcaat cccacgagcc
    36241 gcgcccagac gggcgcggga ttgtggttgt tgggtcatcg cggtagcaag tgaaccataa
    36301 ggaatattca ccaggctgta gcaaagcccg aggcccatgt aggtcaaata tgcatacact
    36361 actttgctgc tatggctcca gtcggtcggt acccagaata ccagcacgct gaagatcatt
    36421 aacggcgcag taccgaagag taaaaacggg cggaattttc cccagcgggt attcacactg
    36481 tccaccactc gtccggcaaa gacgtcggcg aaggcatcga atacccgcac cagtaacagc
    36541 atggtgcctg ccgcagccgc accgacgcca gcgacgtcgg tgtagtaact caacaggaag
    36601 agcgccccca ttgcgaaggc gaagttattg gcgacgtcac cgaggctgta gccgacgatg
    36661 gtgcgccagg agagttgttg attcattgtt tgcctccctg ctgcggtttt tcaccgaagt
    36721 tcatgccagt ccagcgtttt tgcagcagaa aagccgccga cttcggtttg cggtcgcggg
    36781 tgaagatgcc cttcttgtta ccgccaacgc gcaatatgcc ttgcgaggtc gcaaaatcgg
    36841 cgaaattcca tacctgttca ccgacgacgg cgctgacgcg atcaaagacg cggtgataca
    36901 tatccagcca tgcacactga tactcttcac tccacatgtc ggtgtacatt gagtgcagcc
    36961 cggctaacgt atccacgccg tattcggtga tgataatcgg ctgatgcagt ttctcctgcc
    37021 aggccagaag ttctttttcc agtaccttct ctgccgtttc caaatcgccg ctttggacat
    37081 accatccgta ataacggttc aggcacagca catcaaagag atcgctgatg gtatcggtgt
    37141 gagcgtcgca gaacattaca ttgacgcagg tgatcggacg cgtcgggtcg agtttacgcg
    37201 ttgcttccgc cagtggcgcg aaatattccc gtgcaccttg cggacgggta tccggttcgt
    37261 tggcaatact ccacatcacc acgcttgggt ggtttttgtc acgcgctatc agctctttaa
    37321 tcgcctgtaa gtgcgcctgc tgagtttccc cgttgactgc ctcttcgctg tacagttctt
    37381 tcggcttgtt gcccgcttcg aaaccaatgc ctaaagagag gttaaagccg acagctgcag
    37441 tttcatcaat caccacgatg ccatgttcat ctgcccagtc gagcatctct tcggcataag
    37501 ggtaatgcga ggtacggtag gagttggccc caatccagtc cattaatgcg tgatcgtgca
    37561 ccatcagcac gttatcgaat cctttgccgc gcaaatccgc atcttcatga cggccaaagc
    37621 cagtaaagta gaacggtttg tggttgatca ggaactgttc gcccttcact gccactgacc
    37681 ggatgccgac gcgcagcggg tagatatcac actctgtctg gcttttggct gtgacgcaca
    37741 gttcatagag ataaccttca cccggttgcc agaggtgcgg attcaccact tgcaaagtcc
    37801 cgctggtgcc ttgtccagtt gcaaccacct gttgatccgc atcacgcagt tcaacgctga
    37861 catcaccatt ggccaccacc tgccagtcaa cagacgcgtg gttacaggct tgcgcgacat
    37921 gcgtcaccac ggtgatatcg tccacccagg tgttcggcgt ggtgtagagc attacgctgc
    37981 gatggatccc ggcgtagtta aagaaatcat ggaagtaaga ctgctttttc ttgccgtttt
    38041 cgtcggtaat caccattccc ggcgggatag tctgccagtt cagttcgttg ttcacacaaa
    38101 cggtgatacg tacacttttc ccggcaataa catacggcgt gacatcggct tcaaatggcg
    38161 tatagccgcc ctgatgctcc atcacttcct gattattgac ccacactttg ccgtaatgag
    38221 tgaccgcatc gaaacgcagc acgatacgct ggcctgccca acctttcggt ataaagactt
    38281 cgcgctgata ccagacgttg cccacataat tacgaatatc tgcatcggcg aactgatcgt
    38341 taaaactgcc tggcacagca attgcccggc tttcttgtaa cgcgctttcc caccaacgct
    38401 gatcaattcc acagttttcg cgatccagac tgaatgccca caggccgtcg agttttttga
    38461 tttcacgggt tggggtttct acaggacgta acataaggga ctcctcattc aaataaaaat
    38521 accggtcaac ctttaatctg attagttaac caattctgtg ttgcacacag ttatctgtga
    38581 gaaacccatg gaagagaaat ggaaataaaa agaactgaca ccgcgatcaa aaaaccaaaa
    38641 actgcaatac ccacagcgtg atatagatcg cattaatctt taaaacggcg ttgacagaag
    38701 ccataaaata agcaaccgaa cgctcgttcg agtatattgg gaacgataaa ctctcatgat
    38761 atatggctac ggtgaatatt ttagggagga attccccgct ccggcgagat tgtattttgt
    38821 acagggaaat taaagtatga aacgcgggct cagttgtcac cagcccgcgg aaagagagag
    38881 agaatcagga tgcggttaag ataccgccaa tcatcgcgcg taatccctga gcaaacgacg
    38941 cttcacgcgc caggtcttcc gcttcgatat cggccagtac accgtaggtc agatccagca
    39001 gtaaacgtga agtcattgcc gtgttgatgt ctgggcttat ctcgcctttt tgctgggcat
    39061 cacgcatccg ctgagcaaca aattcagtga tcgtcatatg cttattttta agcatggcgg
    39121 cgacctgtgg gttacgcatc ccttccgcca taatttcgac caccagcgcc cgttgcccaa
    39181 aggcttcatg ggtgagagag acaatagact cgaccatata gtcaacaaaa tgaatccctt
    39241 caatcggttc ccggaaacga gccagcgccc tctcctggtc ctgtaagata atcgcctgaa
    39301 tcaaggcttc tttggagatg aaatggtgat agagcgtccc gggactaatg gcgcaagatt
    39361 tacagatggc tttcatcgag gcactgtgaa atccattttc tgaaaaaatc tctctggcag
    39421 cattgaggat ccgggtccgt gtcggttgtg cttcagtctg catgttatcc atcattaaac
    39481 cattatagtt agcgtgaata actagtttac cgcgttgtca ggtgagcgcc tatcattgct
    39541 gttgaatagt gcgaaggcac actctattcg tatacttttc acccagcaat tggttaacca
    39601 atagccgtga tttggctgcc agttgtttag cagagacgtt gatgagttgc tcacgctatc
    39661 ccggtaaaca acacgtacgg ctgtttaccg gtccgttagt gtattaattg agctcctgta
    39721 ccccaccacc ggagacggtg agaatttgtc cgcttaccca gctggctgca ggcgagcaaa
    39781 ggaacagcgc cgcgttagca atatcttgcg gttggcccag acgtctgatc ggcgtgtgct
    39841 gtaacatttt ttgttcaatt tctggtgtaa taacggattt cagggcatcg gttaatattg
    39901 cccccggcgc aatgccattc acccgaatat ttttttcacc caggtcaaat tccatatttc
    39961 tgaccagatg actggccgca gctttagatg acgcatagga agtcatgttt atatttttat
    40021 tttctgccgc catagaagtg atggtcagaa taacgccacc gccatttttt tccatttctg
    40081 gcgcaacaag ttgtgacaga tggaaaaaag aaaacacatt cagttcataa gcacggcgaa
    40141 aatctgccat tggcatatca aacggtttag gaccaccgcc accggcgttg ttaaccagga
    40201 tatcaacttt acccagctta ctgacggcaa agtctgccag tgcagagagt tcctgttcgg
    40261 aagtaatatc acaacgccag gcaaatgcct gaccacccag ttgttgaatt tcgtctacaa
    40321 catggttagc tgcgtcggcg ttaatatcac tgaccaccac agatgcgcca gctgtcgcga
    40381 atgtaatggc gatttcttta ccaatacctg cacccgcacc tgtgatgatg gcgcattttc
    40441 cgtcgagtct caggttgtca gaattaaaca cataaacctc ctgtgaactt cagtaaaaat
    40501 aaaaggcacc gcctgtgtgc cacatttttt atcattaacc ttgaggaaag gacactacca
    40561 ggtatagcag ataaagagag gattatttcg ccgcaatgag ccgggcggga ataataagat
    40621 tgcgtgaatg cgtctcttca tgggtgattt tttgcatcat gcgatccgca agtgtgatac
    40681 caagttcccg cgctggcgtg ctggcccagg taacgggtat atcatcaagt gtggttggtg
    40741 tcgcatcggt aaatgccgcc agcgaaacct gttgctcaaa gtaacgatcg actccgcttt
    40801 ccccgctttg ccgccctgct ttcagcaaac caaaccatgc ccccatcgca atagtttcgt
    40861 tatagcaaac cacggcactg atggtcgggt tatgacgtaa aagcgccgtg atagcttccg
    40921 cggcttgctt ctggctggaa gtgcactcca acacccaatc gctgtgaaac ggcaggccaa
    40981 attttagtag agttgcacaa tagcccccca cccgttctgc acgggttaat gaggaactta
    41041 gccctcccag ccaggcgatc cgctgatgcc cattgcgaat gagatgctcc gtcaacaact
    41101 gtgcagcctg catgttgtcc gggcgaaccg tatcaacatc atcaagataa ctggcacggg
    41161 aagcgaaaat caccgggata gctttttctt ctgccatccg tcgcaggtca tcgctgcttc
    41221 ctgcagcccc ggcaattacc acaccatcga caccctgatt cagtaacagt gaaaaccgct
    41281 gtgccagctg ctcgccgtct ttaccgccgt gaagcaaaaa aaccatccgt ccctgcgctt
    41341 ccagagcttc cgtcaatccg gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga
    41401 cgatcaaacc aatgacgccg ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca
    41461 caaatcccag ctcttcaatg gcggcgttaa cgcgttctcc tgtggcggta gagattcgtc
    41521 ctttgccact aagcaccagc gaaacggtac ttaccgacac gcccgcagcc agcgcaacat
    41581 catgaatggt tatttttttg gcggtagcca tgcagatgac ctactccctg attatgtgac
    41641 agataaaacg ttttaccttt tatttatctt atacccgcta ttatcgttgc gtaatgtgat
    41701 ttatgcctca ctaaaatttg ataaaacgtt ttatcttctc gcgcaattta ctgaatccag
    41761 attgttctct acgaggagtc gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg
    41821 ggagttcttc cagcagttag gcaaaacctt catgttaccc gtggcattat tgtcgttctg
    41881 cggcattatg ctcggcattg gtagttctct tagcagccat gatgtcataa ccctgatccc
    41941 ggtcctgggc aaccccgtgt tgcaggctat ctttacctgg atgagtaaga ttggctcgtt
    42001 tgcttttagt ttcctgcctg tcatgttctg tatcgccatc ccgctgggcc tggcacgcga
    42061 aaataaaggc gtagcggcat tcgctggctt cgtcggttat gcggtaatga acctcgcggt
    42121 aaacttctgg ttgaccaata aaggcattct gccaaccacg gatgccgcgg ttctgaaagc
    42181 caataacatc cagagcattc ttgggatcca gtcgatcgat accgggatcc tcggtgcggt
    42241 gatcgccggt attatcgtct ggatgctgca tgagcgtttc cataatatcc gcctgccgga
    42301 tgcgctggca ttcttcggcg gtacgcgctt cgtaccaatt atctcctcgc tggtaatggg
    42361 ccttgtcggc ctggtgattc cattagtctg gccgattttc gccatgggta ttagcggctt
    42421 gggccatatg ataaacagcg cgggtgattt cggaccgatg ctgtttggta ccggtgaacg
    42481 tctgctgttg ccgtttggtc tgcatcacat tctggtggca ttaattcgct ttaccgacgc
    42541 aggcggcacg caggaagtct gcggtcaaac cgtcagcggc gcactgacca tcttccaggc
    42601 gcaattgaat tgcccgacca ctcacggttt ttctgaaagc gccacgcgtt tcctttcgca
    42661 aggtaaaatg cctgcgtttc tcggcggtct gccaggtgca gcgttagcta tgtatcactg
    42721 cgcgcgcccg gaaaatcgcc ataaaattaa aggtctgctg atttctggcc tgatcgcctg
    42781 cgtcgttggc ggcactaccg aaccgctgga attcctgttc ctgttcgtag cgccagttct
    42841 gtatgtcatc cacgcgctgt taaccggcct cggcttcacc gtcatgtctg tgctcggcgt
    42901 caccatcggt aataccgacg gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg
    42961 tctgtcaacc aagtggtaca tggtgccagt ggtggcggca atctggtttg tcgtttacta
    43021 cgtcatcttc cgtttcgcta tcacccgctt caatctgaaa accccggggc gcgatagcga
    43081 agttgccagc tcaatcgaaa aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt
    43141 tcctgcaatc ctcgaagcat taggcggtgc cgacaatatt gtcagcctcg ataactgcat
    43201 tacccgtctg cgtttgtctg tgaaagatat gtcgcttgtt aatgtgcagg cactgaagga
    43261 caatcgggca attggcgtag tacaacttaa tcaacataac ctgcaggttg ttatcgggcc
    43321 acaagttcag tcagtaaaag atgaaatggc cggtctgatg catactgtcc aggcataagg
    43381 ataagatatg ttcgattttt caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg
    43441 ggattatgtc gctgaccgtt tcggcactgc tgacctgtta ccgttcacga tttcagacat
    43501 ggattttgcc actgccccct gcattatcga ggcgctgaat cagcgcctga tgcacggcgt
    43561 atttggctac agccgctgga aaaacgatga gtttctcgcg gctattgccc actggttttc
    43621 cacccagcat tacaccgcca tcgattctca gacggtggtg tatggccctt ctgtcatcta
    43681 tatggtttca gaactgattc gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac
    43741 acccgcctat gacgcatttt acaaggccat tgaaggtaac cagcgcacag taatgcccgt
    43801 tgctttagag aagcaggctg atggttggtt ttgcgatatg ggcaagttgg aagccgtgtt
    43861 ggcgaaacca gaatgtaaaa ttatgctcct gtgtagccca cagaatccta ccgggaaagt
    43921 gtggacgtgc gatgagctgg agatcatggc tgacctgtgc gagcgtcatg gtgtgcgggt
    43981 tatttccgat gaaatccata tggatatggt ttggggcgag cagccgcata ttccctggag
    44041 taatgtggct cgcggagact gggcgttgct aacgtcgggc tcgaaaagtt tcaatattcc
    44101 cgccctgacc ggtgcttacg ggattataga aaatagcagt agccgcgatg cctatttatc
    44161 ggcactgaaa ggccgtgatg ggctttcttc cccttcggta ctggcgttaa ctgcccatat
    44221 cgccgcctat cagcaaggcg cgccgtggct ggatgcctta cgcatctatc tgaaagataa
    44281 cctgacgtat atcgcagata aaatgaacgc cgcgtttcct gaactcaact ggcagatccc
    44341 acaatccact tatctggcat ggcttgattt acgtccgttg aatattgacg acaacgcgtt
    44401 gcaaaaagca cttatcgaac aagaaaaagt cgcgatcatg ccggggtata cctacggtga
    44461 agaaggtcgt ggttttgtcc gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg
    44521 tgtggctgga ttaattaacg ccatccgcgc tgttcgttaa ccccaattgc gcaacgtaaa
    44581 aaatcgttgc gcaatcgtgg atttttaccc tgctttgttt ttataatggt gcgcactttt
    44641 atatccagaa aaagagtgcg accatgattg ataccaccct gccattaact gatatccatc
    44701 gccaccttga tggcaacatt cgtccccaga ccattcttga acttggccgc cagtataata
    44761 tctcgcttcc tgcacaatcc ctggaaacac tgattcccca cgttcaggtc attgccaacg
    44821 aacccgatct ggtgagcttt ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc
    44881 ttgatgcctg tcgccgcgtg gcatttgaaa acattgaaga tgcagcccgt cacggcctgc
    44941 actatgtcga gctgcgtttt tcaccaggct acatggcaat ggcacatcag ctgcctgtag
    45001 cgggtgttgt cgaagcggtg atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc
    45061 aggcgaagct tatcggcatt atgagccgga ccttcggcga agccgcctgt cagcaagagc
    45121 tggaggcctt tttagcccac cgtgaccaga ttaccgcact tgatttagcc ggtgatgaac
    45181 ttggtttccc gggaagtctg ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc
    45241 atattaccgt ccatgcaggc gaagctgccg ggccggaaag catctggcag gcgattcgtg
    45301 aactgggtgc ggagcgtatt ggacatggcg taaaagccat tgaagatcgg gcgctgatgg
    45361 attttctcgc cgagcaacaa attggtattg aatcctgtct gacctccaat attcagacca
    45421 gcaccgtagc agagctggct gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg
    45481 ccagcattaa cactgacgat cccggcgtac agggagtgga tatcattcac gaatataccg
    45541 ttgccgcgcc agctgctggg ttatcccgcg agcaaatccg ccaggcacag attaatggtc
    45601 tggaaatggc tttcctcagc gctgaggaaa aacgcgcact gcgagaaaaa gtcgccgcga
    45661 agtaacaaaa tggatggtgc aaattgcacc atccattttt catgcaaggc acaaagtcgc
    45721 gcgatgtttg gcggattcga tgcccagctc aatcaactcc attacctgga ttgcctggct
    45781 tgccggaacc ggattttcac catcgccatt taacgcatca cgaatagccg catagtaagc
    45841 cggataattc ccaggcaccg tcaacagcgt ttcttcgaca cgttcctcac cttccacgcg
    45901 ggtaagtacg ccatcacgca tatcgtagcc ccagtcttcc tgcggcagac gctcgccatt
    45961 tttcagacgt tcttcctgtg gatcgaggcc atatttcaca taactgcctc gggatccatg
    46021 cacgatatac cgtgctgact cagcggctgc cagcatggta ccgtgtaaaa tgactcgccg
    46081 ctgtggatag gacaagatgg cgtggaaata atcggtcgac tgcgctccgg gccgtaactg
    46141 tgccaaatct accgtcatgc tgaccggtaa accaaatagc gtaatggcct gatcaagaag
    46201 atgcggtgct aaatcgtacc agataccgct gcctgggccg ccctgttcac gccaacgatc
    46261 gcgcacctgc ggacggaagc ggtcaaaatg agactcaaag taagcaactt cacccagcac
    46321 gccttccgcg agcaaacctt ttagcgtcaa gaaatcgcta tcccaacgac ggttatggaa
    46381 tacagacagc acacgcccca ggctttttgc cagcgcatcc agctctcgcg cttgtgacag
    46441 tgtcacggta aagggtttat caacgaccac atgtttaccc gcctcaagcg ccgctttggc
    46501 taacgggaaa tgggtatcgt tgggtgtagg aatgacaatc aggtctatgt tgggatcgtt
    46561 aaacagatgc ttcggctcag agacaaccgt aaccgttggc cagtcggctt ttacttttgt
    46621 ttcatcactg ctggagatta ccgccagttc cagcccgggc gtgcccgcaa tcaggggcgc
    46681 atggaaggtt ttgctcgcat aaccataccc aatcaaccca acacggatgt tgtcgctcat
    46741 gtcattgcct ctcattgaag tatgatggct atttgacact atcctttacc cacgctcaac
    46801 agtttaataa cctgccagca ataagggatg ttgtttaact taagtcaaaa aaatagcgaa
    46861 ttttccaacg acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat
    46921 tattgctaag taacatttac cccctgaagt taatggatca atcaacagag atgtgggctg
    46981 taatgaatcg tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc
    47041 ttggcgtggc gagtcacatt gacaactatc agccacctga acagagtact acggtacaac
    47101 acaagtaagc tctgcgcttg tagagcgcca tgctgcccgt ccgggtgcat gttttcactt
    47161 gtcggatatt aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat
    47221 agtaaagcgc gttactcttt tcatagcagt ttttcatccc tgaatatttg ccatcagcgc
    47281 attcttatta ttgtaataac acaagcgttc acttttggtg aaattttatc atcactaata
    47341 atacctaccc ctttggtatt aattatttta tggacctttt atgactgttc aggactactt
    47401 attaaaattt cgcaaaatca gttcactcga aagtctggaa aaactctacg accatcttaa
    47461 ttacaccctg acggacgatc aggaactgat caatatgtat cgtgctgccg atcaccgtcg
    47521 cgcagagctg gtttctggcg ggcgtttgtt tgacctcggc caggtaccga agtccgtctg
    47581 gcactatgtc caataaagaa agtagcgatc ggttataaaa gccttataat aacgccccta
    47641 aaattagatt tgcctgtttg cgtcgctatt ctggagaacc tatgaccacc acaacgccac
    47701 aacgtattgg aggctggttg cttggcccgt tggcctggct tttagtcgct ctgttaagta
    47761 cgacgctggc gttgttgctg tataccgctg cgttatcttc tccgcaaaca tttcaaacgc
    47821 tcggcgcaca agcactgacg acacaaatct tatggggcgt ttcttttatt accgctatcg
    47881 ccatgtggta ttacacactg tggttgacca tcgctttctt taaacgtcgg cgttgtgttc
    47941 ctaaacacta tattatctgg ctgcttattt ccgtattgct ggcagtaaaa gcattcgctt
    48001 tttcacccgt ggaagatggc atagccgttc gccagttgct gtttactttg ttggcaaccg
    48061 cattgatcgt cccctatttc aaacgctcgt cgcgggtaaa agcgacgttt gtgaatccgt
    48121 aataacctta cagttaacct gttgtcgcct gctctggatt aacggataat aggcggcttt
    48181 tttatttcag gccgaaaaat gactgactac ctgttactgt ttgtcggaac tgtactggtc
    48241 aataactttg tactggtcaa gtttctcggt ctctgtccgt ttatgggggt ttccaaaaaa
    48301 ctggaaaccg cgatgggcat ggggctggca acaacgtttg tgatgacgct ggcgtctatt
    48361 tgcgcctggc ttatcgatac gtggattttg atcccactta atctgattta cctgcgcacc
    48421 ctggcattta ttctggtgat tgctgtggtc gtgcagttca ccgagatggt ggtgcgcaaa
    48481 accagcccgg tgctttaccg tctgctgggg atttttttgc cgcttatcac caccaactgt
    48541 gcagtgctcg gcgtggcgtt gctgaatatc aatctcgggc acaatttctt gcagtcggcg
    48601 ctgtacggtt tttccgccgc tgtcggtttc tcgctggtga tggtgctctt cgccgccatc
    48661 cgcgaacgcc ttgctgtggc tgatgtcccg gcaccttttc gcggtaatgc cattgcgtta
    48721 attaccgcag gtcttatgtc tctggccttt atgggcttta gtggtttggt gaagttgtaa
    48781 tgaatgctat ctggattgcc gttgccgccg tgagcctgct gggcctggcg tttggcgcca
    48841 ttctgggtta tgcctcccgc cgttttgcgg tggaagacga tccggtcgtt gagaaaattg
    48901 acgaaatctt accgcagagc cagtgtggtc agtgcggtta tcccggctgt cgcccctacg
    48961 cggaagccat cagctgtaac ggtgaaaaaa tcaaccgttg cgccccaggt ggcgaagctg
    49021 tgatgctaaa aattgccgag ttgcttaatg tcgagccgca gccgctggat ggcgaagcgc
    49081 aagagctaac gcctgcgcgg atggtggcgg ttattgatga aaataactgt attggctgca
    49141 ctaaatgtat tcaggcttgt ccggtagacg ccatcgttgg cgctacccgt gccatgcata
    49201 cggtaatgag tgaactctgt acgggctgca atttatgtgt tgatccgtgc ccgacgcact
    49261 gcatctcgtt gcaaccggtc gcagaaacac ctgactcctg gaaatgggat ctgaacacca
    49321 ttcccgtgcg tatcattccc gtggaacacc atgcttaagt tattctctgc attcagaaaa
    49381 aataaaatct gggatttcaa cggcggcatc catccaccgg agatgaaaac ccagtccaac
    49441 ggtacaccac tgcgccaggt accactggcg cagcgttttg ttattccact gaaacagcat
    49501 attggcgctg aaggtgagtt gtgcgttagc gtcggcgata aagtattgcg cggccagccg
    49561 cttacccgtg gtcgcggcaa aatgctgcct gttcacgcgc ccacctcggg taccgttacg
    49621 gctattgcgc cccactctac ggctcatcct tcagctttag ctgaattaag cgtgattatt
    49681 gatgccgatg gtgaagactg ctggatcccg cgagacggct gggccgatta tcgctctcgc
    49741 agtcgcgaag agttaatcga gcgcattcat cagtttggtg ttgccgggct gggcggtgca
    49801 ggattcccga caggcgttaa attgcagggt ggcggagata agattgaaac gttgattatc
    49861 aacgcggctg agtgcgagcc gtacattacc gccgatgacc gtttgatgca ggattgcgcg
    49921 gctcaggtcg tagagggtat tcgcattctt gcgcatattc tgcagccacg cgaaattctt
    49981 atcggcattg aagataacaa accgcaggcg atttccatgc tgcgcgcggt gctggcggac
    50041 tctaacgata tttctctgcg ggtgattcca accaaatatc cttctggcgg tgctaaacaa
    50101 ttaacctaca ttctgaccgg gaagcaggtt ccacatggcg ggcgttcatc cgatatcggc
    50161 gtattaatgc aaaacgtcgg cactgcttat gcagtgaaac gtgccgttat tgatggcgag
    50221 ccgattaccg agcgtgttgt aaccctgact ggcgaagcaa tcgctcgccc gggcaacgtc
    50281 tgggcacggc tggggacgcc agtgcgtcat ttattgaatg atgccggatt ctgcccctct
    50341 gccgatcaaa tggtgattat gggtggcccg ctaatgggct ttaccttgcc atggctggat
    50401 gtcccggtcg taaagattac caactgtctg ttggctccct ctgccaatga acttggcgaa
    50461 ccacaggaag aacaaagctg catccggtgt agcgcctgtg ctgacgcctg ccctgcggat
    50521 cttttgccgc aacagttgta ctggttcagc aaaggtcagc aacacgataa agctaccacg
    50581 cataacattg ctgattgcat tgaatgtggg gcttgcgcgt gggtttgccc gagcaatatt
    50641 cccctggtgc aatatttccg tcaggaaaaa gctgaaattg cggctattcg tcaggaagaa
    50701 aagcgcgccg cagaagccaa agcgcgtttc gaagcgcgcc aggctcgtct ggagcgcgaa
    50761 aaagcggctc gccttgaacg acataagagc gcagccgttc aacctgcagc caaagataaa
    50821 gatgcgattg ctgccgctct ggcgcgggtg aaagagaaac aggcccaagc tacacagcct
    50881 attgtgatta aagcgggcga acgcccggat aacagtgcaa ttattgcagc acgggaagcc
    50941 cgtaaagcgc aagccagagc gaaacaggca gaactgcagc aaactaacga cgcagcaacc
    51001 gttgctgatc cacgtaaaac tgccgttgaa gcagctatcg cccgcgccaa agcgcgcaag
    51061 ctggaacagc aacaggctaa tgcggaacca gaagaacagg tcgatccgcg caaagccgcc
    51121 gtcgaagccg
//
LOCUS       DAOFQD010000020        49430 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.020, whole genome shotgun sequence.
ACCESSION   DAOFQD010000020 DAOFQD010000000
VERSION     DAOFQD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.020"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..552)
                     /locus_tag="QBF46_001219"
     CDS             complement(<1..552)
                     /locus_tag="QBF46_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HDQ6673428.1"
                     /translation="MAIHDAGMFTVKEINRLKILQDVVERNLRPGQAAEMLGITPRHC
                     SRLLKRYRQSGPLGMNNQSRGRTGNRLLPTSLTDQALSIIRERYRDFGPTLAREKLEE
                     VHGLVLGKETIRRLMIKAGLWIPRRQRAPKIHQPRYRRPCTGELIQIDGCDHHWFENR
                     GRPCTALVYVDDATSRLMHLLFVK"
     gene            1047..1973
                     /gene="tcuR"
                     /locus_tag="QBF46_001220"
     CDS             1047..1973
                     /gene="tcuR"
                     /locus_tag="QBF46_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization LysR family
                     transcriptional regulator TcuR"
                     /protein_id="HDQ6673429.1"
                     /translation="MELRQLRYFVRIVETGSMGSAALDLDIGVSALSQQMTRLENELA
                     IRLLQRTSRGVTPTNSGLAFYSQAQLALRHADDAILAARDARLSGHVSVGMAPSTASV
                     LGVPFIHAMRAHYADVRLHVVESLSGNLERMINTRQIDLAVVFRKEKHLRWSARPVLE
                     EQLFLIGTHDLLTDIPDDTITPAQLANIPLIMPSSGHGLRGLLEAISQEHGLKVDIAT
                     EIDGLALLMRAVGSGLGATLQPGAAISHLESESLRVIGIHNPILSRPNFLVSLSDDEL
                     PPAALAARVILTKVMRKLVEIGHWPGATIYAP"
     gene            2071..3474
                     /gene="tcuA"
                     /locus_tag="QBF46_001221"
     CDS             2071..3474
                     /gene="tcuA"
                     /locus_tag="QBF46_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HDQ6673430.1"
                     /translation="MVDVLVIGGGNAALCAALTAREAGASVLLLEAAPREWRGGNSQH
                     TRNLRCMHDAPQDVLVESYPEEEFWQDLWRVTEGNTNEALARLVIRTSSQCRDWMRKH
                     GVNFQPPLSGALHVARTNAFFMGGGKALVNAYYRSAEKLGVQIRYSTPVQALELHNGK
                     FVAALAGHERIEAKACVLAAGGFESNREWLRDAWGENTRGEWPADNFLIRGTRFNQGV
                     LLKFMINAGADIIGDPSQSHCVAIDARAPLYDGGICTRVDCVSLGVVVNRDAERFYDE
                     GEDFWPKRYAIWGRLVAHQPGQIGYSIIDSKAIGHFMPPVFPGAQANTLRDLARQLGL
                     DPEHFTHTVEHYNQACQLGQFDHTKLDNCATQGLTPPKTHWARPIDTPPYYGYALRPG
                     ITFTYLGLKVNEHAAVHFAGTPSCNLFVAGEMMAGNVLGKGYTAGVGMSIGTTFGRIA
                     GKEAAQAARKEAHHEAA"
     gene            3461..4600
                     /gene="tcuB"
                     /locus_tag="QBF46_001222"
     CDS             3461..4600
                     /gene="tcuB"
                     /locus_tag="QBF46_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HDQ6673431.1"
                     /translation="MKQLEKLIIEAKILTEPEAEVERVMQVCNACRYCEGFCAVFPAM
                     TQRLEFGKADINYLANLCHNCGACLHACQYAPPHEFAINVPKAMAEVRLETYQHYAQP
                     AAFGALYRQAGVTVTLALVFSLILFLLLAMGLKGSLLHPPLAGDFYQIFPHNLLAWMF
                     GSVFMLAIGLLMAGVIRFWREISPVGPRPAEIAEASHNALTLKYLDGGHGKGCNEADD
                     AFTLMRRRFHHFTFYGFMLCFAATVVATGYHYFAGWEAPYPFFSVPVMLGTLGGIGLI
                     VGPAGLLWLNLKRSSLHGDARQKPMDRGFILLLLLTSLTGIGLLAGRDTSWMGILLAI
                     HLGVVMALFLTIPYGKFAHGFYRCASLLKWAIEKRRGKQAGVAGD"
     gene            4655..5950
                     /locus_tag="QBF46_001223"
     CDS             4655..5950
                     /locus_tag="QBF46_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6673432.1"
                     /translation="MTQQPSRAGTFGAILRVTSGNFLEQFDFFLFGFYATYIAKTFFP
                     AESEFAALMLTFAVFGSGFLMRPIGAVVLGAYIDRIGRRKGLMITLAIMGCGTLLIAL
                     VPGYQTIGLLAPVLVLVGRLLQGFSAGVELGGVSVYLSEIATPGNKGFYTSWQSASQQ
                     VAIVVAALIGYGLNVTLGHDEISEWGWRIPFFIGCMIIPLIFVLRRSLQETEAFLQRK
                     HRPDTREIFTTIAKNWRIITAGTLLVAMTTTTFYFITVYTPTYGRTVLNLSARDSLVV
                     TMLVGISNFIWLPIGGAISDRIGRRPVLMGITLLALVTTLPVMNWLTAAPDFTRMTLV
                     LLWFSFFFGMYNGAMVAALTEVMPVYVRTVGFSLAFSLATAIFGGLTPAISTALVQLT
                     GDKSSPGWWLMCAALCGLAATTMLFARLSSGYQTVENKL"
     gene            6461..6676
                     /locus_tag="QBF46_001224"
                     /pseudo
     CDS             6461..6676
                     /locus_tag="QBF46_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534813.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6710..7135
                     /locus_tag="QBF46_001225"
     CDS             6710..7135
                     /locus_tag="QBF46_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673433.1"
                     /translation="MTDQASIPVDTPVLALATDAYSSLKNILNDNGTSDTTGTCMFAS
                     LLVCEFAHRRGMSAAVRGGNGTDDGGIFNESGGHGHYWCEVSAGEMIFYIDIAAEQFG
                     YPSFIIKNANDVSGWPRYIPGDQVTVDEHVRITLSGGIR"
     gene            complement(7337..7558)
                     /locus_tag="QBF46_001226"
     CDS             complement(7337..7558)
                     /locus_tag="QBF46_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673434.1"
                     /translation="MPKKNLYSEEIVIAKKQLTQLSTLKDKVRLLTRQWEGDIGANLP
                     GYTELLAHVERIERQIHEQIGSWKKTPVM"
     gene            complement(7702..8127)
                     /locus_tag="QBF46_001227"
                     /pseudo
     CDS             complement(7702..8127)
                     /locus_tag="QBF46_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502663.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8403..8756)
                     /locus_tag="QBF46_001228"
     CDS             complement(8403..8756)
                     /locus_tag="QBF46_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673435.1"
                     /translation="MKIKNIVLAGIISLVFSVHASDKQNIKRDPIPYLGYDAVTSNMC
                     IDAGGLTDPVEMRKKATETLLMILHKQPGDKLSKQSEDTIEAFVTRTRSSSDDISKCQ
                     FSVYERYWEIVQPNK"
     gene            complement(8904..9518)
                     /locus_tag="QBF46_001229"
     CDS             complement(8904..9518)
                     /locus_tag="QBF46_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673436.1"
                     /translation="MAIDTDDNDPVDAEACEKYLAQLRELEAYRAYRTTAAIDWFFDQ
                     ATRAIHGELWLAACTTFLNGIETSLRVTMKLKASQAQPQAPTPLVDLSDMATLSNALL
                     RRAHQAGMPVTLLAFPDEQDLLTKIADGAPKLPYAEIVRVRHNLCHGNILEHIITASD
                     GMGEPVRLFTPECMRDLAQTLSAVSKVWIAGLHQYWCDNNLSMP"
     gene            9696..10130
                     /gene="umuD"
                     /locus_tag="QBF46_001230"
     CDS             9696..10130
                     /gene="umuD"
                     /locus_tag="QBF46_001230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDQ6673437.1"
                     /translation="MSVFPERFASPPRDTTPFFSDLLSCGVMSPCAGHEDNELNLHDF
                     VVRNRPATFFVRASGLSMVDAGINDGAILVVDRSLTAEHGSIVVALVDGEFTVKILQT
                     FPKLMLMPSNPAFKPIPVNPDTLEIWGVVTFALNQFPHVHAR"
     gene            10114..11373
                     /locus_tag="QBF46_001231"
     CDS             10114..11373
                     /locus_tag="QBF46_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDQ6673438.1"
                     /translation="MYMHVDINGAYAAFECAMDPKLAKSPLIIASNNDATIIAMNRLA
                     KNTGLKRGMPIFKCRDLIEQHHIAVRSSNFTLYEDYSNRFHETLEGFAEDTDRYSVDE
                     SFMLLKNMDKIVDFEEYGCLIRRTLLHNLSLTCGIGVSTTKTLAKLCTYASKRWAATG
                     GVVVLTDPFRIKKLLSLISTREIWGIGRKISERLAAYGIITACDFYDSDVRFLRKAFG
                     VEMERTWRELHGESCFRLHESPPVRQQIIVSRSFGQRLNTFSKMHEAVSFFTGRAAEQ
                     LRNDGSWTRQITVFIQSSPFARAETRYSSCGIEPLTATQDTRDLVSAAGAILTRIYKP
                     GIDYAKAGVMLSSMTDGTEQLSLFDERPARRGSEALMKVMDRFNKEHRGSLFLACEGI
                     HQDFRGKQAMLSPRYTTRWNELLVVKA"
     gene            complement(11425..12234)
                     /locus_tag="QBF46_001232"
     CDS             complement(11425..12234)
                     /locus_tag="QBF46_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDQ6673439.1"
                     /translation="MIKLNYLKPVASVLISAVIATAGSVYITSKYLKPDVPGNDEIGR
                     VAASYLVKNPQYLLEAGKALEKENTNASLERIIPYAPALFETKETPNIGPDNAAVAVI
                     EFFDYQCHFCMQVAPVVESVLSQSTDVKFFFKEFPIFAGSKPVSAMGAATGLHVYQTF
                     GAEAYRKYHNNLMTSAYVFFNNQRAFTLNDLDMVVNKSGFNSSFGDREKSRYENVISG
                     NMQLGEALGINGTPGFIIMNMQKPDAATTSFIPGAVDEATLKYAIQKARGG"
     gene            12341..12952
                     /locus_tag="QBF46_001233"
     CDS             12341..12952
                     /locus_tag="QBF46_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6673440.1"
                     /translation="MLRQFFLLTATTFLCGCGYHFGNDVDAYDLLPRPVGSKKFEIVA
                     PDDSIQSRMFAARFASGLTGKGFNISSHQPEYILRFSYSKTQENLQYSELPVTGITGY
                     VIEKKTTRKDKHGQTETDYDYKPVSGIVGTETVSQRHFWRRLDVEVYPAGKNAQQVLK
                     VSMQSNAPIPSDSVAYSAMIDALTGNLDAPLRSGNYVASVPWN"
     gene            complement(13011..13433)
                     /locus_tag="QBF46_001234"
     CDS             complement(13011..13433)
                     /locus_tag="QBF46_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673441.1"
                     /translation="MSLWLSIVTRATGLPDKRYLNDPVILRYYQQKIQKAPYALVGWT
                     LCFMATIGVSLGLDLSNQMLLLVLLILIFLMVVLQGRYQFMHPERCINAKFYQTVLDD
                     ASVSDIQKAQFEYLFVTKVDELTIGDLYRLYDFNRGGR"
     gene            complement(13456..13848)
                     /locus_tag="QBF46_001235"
     CDS             complement(13456..13848)
                     /locus_tag="QBF46_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673442.1"
                     /translation="MKSAILTTLMLVAVGSQPVFAAKCQFGACDADTVASSESLVSAA
                     QTDKDAVLKKLNDATSRSDVMSKRFESYLSNDRLKVNTDSTKKGPSNEIAKPRDELAP
                     VRQLKAIRPELVQTPIFQPIVAFYERLA"
     gene            complement(13874..14623)
                     /locus_tag="QBF46_001236"
     CDS             complement(13874..14623)
                     /locus_tag="QBF46_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HDQ6673443.1"
                     /translation="MNKTIKASLFLLASCTALSMNAQTANTDVLPDAALKKLREVGLS
                     IEHIEPSPVKDIYTVISREGVSYVSKDGDYIFTGSLFHVNGKDVENTTEQAILKGVRE
                     FATKTKSIEYKSANEKYRLAVFTDITCGFCQKLHQDLQSYLDAGISIKFLAFPRAGLN
                     SVVAGNMAKIWCAAKPNDALDAAMSPTSTIPEGRPDEACLDTIKSHFQVASTIPLQGT
                     PTMVTLSGKPQLFTGWMSPENLVTRMGAEQK"
     gene            14771..15286
                     /locus_tag="QBF46_001237"
     CDS             14771..15286
                     /locus_tag="QBF46_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDQ6673444.1"
                     /translation="MFIPPVDDVKPIPVPVEIYTQCITDASRFFGIDAELVFTLFDNE
                     GGKVGTFSRNKNGTYDIGPMQINSSNLPEIRDHFPSVTWRVLAYDACASFWVGTWWLY
                     RKIVDRKGNVFEGIADYNSKTPKVRATYIFNFMIKYNRRIQRRNGMDELYQWTQPKPQ
                     YNGHIVKNLPE"
     gene            15383..15949
                     /locus_tag="QBF46_001238"
     CDS             15383..15949
                     /locus_tag="QBF46_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673445.1"
                     /translation="MLQSIYKFLFNLCSSPQLPKNKDVDGILSHEEITAGLWGPLPSI
                     HVVLFLDFDGVVHKCQNESFERMHLIERLLDASPSMFIVISSSWRECGSITYLKSLFR
                     SPYRDRVIGATPSLYLPSGSIGVRAAECEDFVTRHKVKAFICLDDDATLFPPEYPHLF
                     RTDYYTGVNEKDLTDLIARYNVLMTRFY"
     gene            complement(16196..17044)
                     /locus_tag="QBF46_001239"
                     /pseudo
     CDS             complement(16196..17044)
                     /locus_tag="QBF46_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
     gene            complement(17047..17364)
                     /locus_tag="QBF46_001240"
                     /pseudo
     CDS             complement(17047..17364)
                     /locus_tag="QBF46_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17452..19836)
                     /locus_tag="QBF46_001241"
     CDS             complement(17452..19836)
                     /locus_tag="QBF46_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDQ6673446.1"
                     /translation="MKFCRRPLIVAVYTALLFPLYSQAQLYNYSYTDTRGTKVNAEPT
                     REYLNPGSAITLNLISGLDRYERVTVTRSSDNVQMFQSVTGLVTVADRITAADGSEYY
                     GKSMTLPVLGEGTFSLKSETLDNKSNVVATTSYSFSIDITPPVLPDPMQWIRAGFQYG
                     SLDIFGDRTATQAISLSNVSDARSGLDKAEWFAIDSSGVRRTVNAQLNALTGVVQALA
                     AVAAGSTVAPVSPAYYTVGFRVYDKAGNWTETSHRSGIDRTVPSYIQHQVRNAKTGVW
                     EDYVPGMTIYENPVKFRFKRLKSDSTNFNGTDFGWIDQITNSDSTYFYTEVTVSYPET
                     YVYYERYTKAGLNNNINYSTLKFTLGSGVPEAPRYLSGQFHFKKGNSWTPTDIVRFTG
                     PDCVDMGRVNVEPRPYVQTATIIGLGSCNVAVGESSCLVSMDYCKTSGKGYSPYQIHM
                     KGTGTYANLGGLQSHFYTYWDMNPPVINSIVSNPLDKTVVMLVTDSDVTNDWTTYMWA
                     TNIFNLSLKDTSNKVTTLSPKSVAVLNYQLKQVTFDYSKLPSGKYTATGAATDTYNNT
                     AELKLSSTILHDVTPPVVGIIFEGKPADGSLVKGLENLVITLSDDMTKASLKNITLSG
                     GPTSDKVSVGWYSQGTDKYGLNYPRIFPATDDASSYLLTVVAMDEAGNATTKTSRFRY
                     VPNNLIEFNTIKTLAVGMGLKTSDNQPLAYLRTNSIRKKDGSLITGAQTGTLTVRKDA
                     AFAVSMNGVAVVPGDSKDITIDFGQGDGVLIPIFPATSGNVGESSFMIELPQIQ"
     gene            20087..20689
                     /locus_tag="QBF46_001242"
     CDS             20087..20689
                     /locus_tag="QBF46_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDQ6673447.1"
                     /translation="MKNQAIALAMAMLGVSVPASAQIYESIFTDTNGIEVHAPSTRLL
                     LNPASPVTLTLIAGLDRYVNVKITSATGTQLLNTTTTRTSVSDRLKAGDGSEFYGKKV
                     TLPVLGEGRSVVQVSILDLNQSVVATYSYNWLVDTTPLSVNALTGNAAYGSTAGSVWK
                     MGLEATGQFDFTSTNVTDVNGIEKGMFYVYRSNGSLFSTT"
     gene            20701..20937
                     /locus_tag="QBF46_001243"
     CDS             20701..20937
                     /locus_tag="QBF46_001243"
                     /inference="COORDINATES: protein motif:HMM:NF023666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HDQ6673448.1"
                     /translation="MYHTYSKNSVKGAGIPDSNLDEDFTAKVVIYDNAGNTRTLPLQL
                     FRYDNYLGGPRSAKLRQRGARGFRISGLFSWDDR"
     gene            21093..21689
                     /locus_tag="QBF46_001244"
     CDS             21093..21689
                     /locus_tag="QBF46_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673449.1"
                     /translation="MVYGLENIRIALADNLTTPRITRLQITGGPTADNVELTWSPAGT
                     NVYAPEYPRLFPSFEPSENYALSVTVSDSQSNSKTYTQKFSYLPNNLVQLHNLRTLSV
                     SSALKTSLNEPLAYLSTNVLRKSNGEIAKDVQSATLTVRKDAAFGIKFNGVQAALGES
                     VEVKIDMGLGDNLLVPIYPAENGKVGTSEFMIQIDELK"
     gene            22081..22962
                     /locus_tag="QBF46_001245"
     CDS             22081..22962
                     /locus_tag="QBF46_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDQ6673450.1"
                     /translation="MSSNNNKLSLVIEESLKDSGEVIQLFPTLKQTIQPKVLLRLGVF
                     VPTLKSTDKGGRKSHHIDATEPLSRLSIVEGEGYSQIQIYGPRLDMDTDFKSWCGIVY
                     ALSNRPLDSEGQLELNFPEFAKFCGFDTKRIDKQLKERFDASLTRIARTNISFIKKIP
                     GTDDQITINMHLVESTYYDSSTGKIIIKPNSKLNTLYRVDGKTRLYLKTLQKLARKES
                     AQALYLYLAELPSTFYRIGFDRLRGRLQLSSHLGNQNATVKKALGQLKEIGFLEYSID
                     KENGDYVLNILKRNMKP"
     gene            complement(23548..24063)
                     /locus_tag="QBF46_001246"
     CDS             complement(23548..24063)
                     /locus_tag="QBF46_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673451.1"
                     /translation="MKISTGMMKSNAPLIILICLMIAFSAYVKSWSLLYTLLAASVSL
                     TVMISLPFIVSPLQHSKFIALRILRLLVITLWTIGLVGVFFSVVERLVWVNADSYPAW
                     LAKELDSPAITDGENFIKATESFEKICGKNKGYLSVVTKHNGIFMRCDDSLSFDSWWK
                     GVYRLKTPETR"
     gene            complement(24714..27170)
                     /locus_tag="QBF46_001247"
     CDS             complement(24714..27170)
                     /locus_tag="QBF46_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDQ6673452.1"
                     /translation="MRKILTGCIIATAIASVYPAAAQIFSYSYTDTNQLAKTLKPATQ
                     SYLNPASTMTLNLISGLDRYERVSVTRDSDKKVMYSAVTGKISVADRIVAADGTEYYG
                     KDLALPALGEGAFTVINDTLDLQQAVVSSSTYHFTIDTTAPKYTSIYPSQNAGYEMVL
                     SGPVWGLGRGGSGQFSIFADGVDDANGIDKIRLVIKRGNGTVVSDNTLSYDTAAKRAF
                     YPWIKDSVTQPGMPTSDLNEEFTFNFIITDKAGNTLNIPPQRFLFDDQIGEYTPFAVH
                     DSRVSTSVVPGISSGYVPFVRGLSVLENPYRMVIRIPRTNWKPYRNGGITILNNYGGA
                     KVISEDTTYVYVEVKLPQGALDINYYRPVNTYQWSGGDLTQYASWLNWDPASVKSPAW
                     GSPAIERQMSDGTWFNSVNWKYFKASDMPVNLTQIRFNVQARPYDQKITGGATCNIPA
                     GSTSCTVALTQAITNGTTGYLHSGYEVRSTTESTFFMPIWENVVWHTLGPSVTGYDYN
                     DTTNILQVYINQPGDGSYFDTVKLSRVWLSDKSRNNADINVTGTLTGRNTATGNYTYE
                     FNLKLIPEGSYNVQLNAQDSFNNTGSLDYKTVTVDNTPPSISISYETKPITSSVTVYG
                     LENIRIQLADTLTKPSLTRMTLRGGPVSDAVELSWVNLGNNLYAPNYPKIFPSLNDGE
                     TYTLTVQAKDEMNNVKESAVEFSYLPNNLVRLENLKTISVSTALKTSDDTPLAVLYAS
                     QLRKKDGSIATGKQDAILTVRKDAAFGVTVNGVSAAPGESKNVQLDLGLGDSRSFPIF
                     PSTSGVVGTSEFMLNIEELK"
     gene            complement(27616..27792)
                     /locus_tag="QBF46_001248"
     CDS             complement(27616..27792)
                     /locus_tag="QBF46_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673453.1"
                     /translation="MSKSSPVLPEDRDDIEFVMDDFQRSLGKSKPVREVQFLGESQQS
                     EESANSADSPWFES"
     gene            complement(27807..29483)
                     /locus_tag="QBF46_001249"
     CDS             complement(27807..29483)
                     /locus_tag="QBF46_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDQ6673454.1"
                     /translation="MSAVESDSLPMGAPGSSLVAYMPHTGLLINDDNFLPEMREELDV
                     INRQVESYNDELSLLRSMSAQFLKCGNQKLYSYLSGYNHLISEADASYAENALRSEYW
                     KRVMALTDVLSVMSDEKKKEWDKQFTADRYHLPPQEIPDFTLDAVVTTVVALLNDRHQ
                     FIKDRVYAVFQSLSRQHKTNKAFGFSTRMITSGVCEGVKDKWVKLKVEFKESGLMPLS
                     ELRVICSYFRGETVKPVYDTKKMVENMVGHVGFRNWISLDGNSIRFRVYKNGSMHIDV
                     HPDIAERLNNILAAIVPLALPAERVAHTKATLQEFPALKRCIDFHSRMQLAELDFTQN
                     EKEWSSWTSLGYAGENADKTRQVNADVLRFLGARVTSYHVTFDYDPTEVMRFIGHMGA
                     MPDIKSHQFYPSSARLSEYVASIIAAGEGERLLEPNIGHGDLLQCFPKSVNVTGIELD
                     TLNCLVSRAKGYETTEADFLAWSAANQIQKFDCVVMNPPFADNRAKLHLLAAASHLVP
                     GGRLAAVLPLSLQGIDNLLGEGFRTEWMDTFDNEFDNTAVSVRVLYAERF"
     gene            complement(29747..30899)
                     /locus_tag="QBF46_001250"
                     /pseudo
     CDS             complement(29747..30899)
                     /locus_tag="QBF46_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            31259..32083
                     /locus_tag="QBF46_001251"
     CDS             31259..32083
                     /locus_tag="QBF46_001251"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDQ6673455.1"
                     /translation="MRKHTPIIKWAGGKTKLMPFLSKHFPHDKSRRWVEPFIGGGAVF
                     LNMFATEALLADSNPDLINLYRNIQRNKPAFIREVQLLAERHFEEEDYYVLRNTFNST
                     SFDDAPLQRAAIFYAMNRLGYNGLCRYNLKRKFSVPWGKRYQFSLDIQKVDYLSFRLS
                     SVELKTADFGQTLEFAGGGDQIYCDPPYDKISKTSFVSYDGIPFDKSAHVKLADMLVD
                     ANRKGASVAISNSMTPFTLELYEERGFDIHTHNAYRSVGSQSKSRKKEIEILAVLR"
     gene            complement(32098..32919)
                     /locus_tag="QBF46_001252"
     CDS             complement(32098..32919)
                     /locus_tag="QBF46_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673456.1"
                     /translation="MQNDLGLGFIRKSARTSAKIISRKVTFPLDDLDNAKLTLTGLQC
                     HPSCRFPFYQLSMLIHNQLAEHGVISADENAPFTVSISIHEWLSRARVAFIDYHGLRN
                     SDKTVMITRAHLEELLTYLSTTLAVYVPYSKKRLNFSFLTSFTLVKTSQSYTLTFPGV
                     LRPFLMLLGGLIQEFMTEKLLKRRNAQFVIFDYLRKSRRHSHKITDIVNDLQLHTVNV
                     RIMNVLTQLADQGFISFICEGKRGERSIEELQFIPYASRDHSEVLSFEEWISPID"
     gene            complement(33202..33909)
                     /locus_tag="QBF46_001253"
     CDS             complement(33202..33909)
                     /locus_tag="QBF46_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673457.1"
                     /translation="MKHSLFFTQDGYLLPSSVKPIYGKDGHYYAVITRECFECGGTGK
                     IANAVCVTCNGSKTSPATEEKLVLKECIEFLETIPELRCNPDLEAWKRRHSEQLALLQ
                     ADNSKFAASIAQQLNNNLFLTVRQLESFYSTYQPVSEAFSENAGFPIEHSFNIGDVVS
                     FDIIFTEVRAAFSKGSHSYFFTFTGKKQNDGFALTGNVQDHVLLGHSYHLSGKVRKIH
                     YFDSVPYALLTVFKLTA"
     gene            complement(33906..34106)
                     /locus_tag="QBF46_001254"
     CDS             complement(33906..34106)
                     /locus_tag="QBF46_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673458.1"
                     /translation="MDKIAQFTVINTYNDLLITKGTLDRNIEEIKAGKRFDHFEEGHD
                     QYVQKVTDALQLVEARLENLKK"
     gene            complement(34124..35200)
                     /locus_tag="QBF46_001255"
     CDS             complement(34124..35200)
                     /locus_tag="QBF46_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673459.1"
                     /translation="MNHCDYQIIEISPKALFIESGFNPREAVIGELCYEQEPVKSTIV
                     SIKQAYKEGRRVDLIKVVKKDSNYVVRQGHCRYRALSLALAEGADIQLISVVVMNYKN
                     KSEEYLENLDGNRNNGLNPVALAYALAEALKLGSTVESLAMRYQRSSTAIRNQLKILD
                     MPLELQRLISLNLIKKTLAIEIMLKYSNDHAKVLAHLESCSLLVPSTEPEPVLSKTSS
                     VQTSSNDEKLDNPETLPVHVIPKEVKTKSITRSSLGIKRLSHKKTERLKTEFIGLIDT
                     VRPALNDSNKLTELIFDGQHSNLFTNISSTSITKTTESVKITLDEAKLQLLLSLLNDE
                     NTAIIIPKSKVTELHEIRDLLTSE"
     gene            35735..36610
                     /locus_tag="QBF46_001256"
     CDS             35735..36610
                     /locus_tag="QBF46_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDQ6673460.1"
                     /translation="MSDNNEVTHPFDVTNTETGKTYQLSPNSSKSVQPIALLRLSVFT
                     PVGTKEKRYRNFEVDASDELSSMELARSEGYDDIRITGLKLSMSTDFKCWLGCIMAFS
                     KYGFASDKITLSFNEFAKMCGISSTNINKRTRSRFQEALANLASVVISFRDSKTERFT
                     VTHLVQKAVIDPKKDTVELVGDPSMWELYRYDHKTLLSLQVLSVLAKKEAAQSLYIYF
                     EAMPAGTLFVSMKRLRERLLLTTPVRTQNQIIRKAMLELKSIGYLEYQEVKKGRDIQF
                     QIFKRSPKLALAKHS"
     gene            complement(37057..37401)
                     /locus_tag="QBF46_001257"
     CDS             complement(37057..37401)
                     /locus_tag="QBF46_001257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673461.1"
                     /translation="MQMLSNQRQLTLPHSFKCFPTKGNFHSPIRANAFRTKAPYTVPC
                     EPCFPTKSNFHRSIHTNAFFKRHFSAAIEANAFIQKALFCRHWSKCFYTKGTFLLSIE
                     TNAFHLLDIQFE"
     gene            37964..38317
                     /locus_tag="QBF46_001258"
     CDS             37964..38317
                     /locus_tag="QBF46_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HDQ6673462.1"
                     /translation="MELTLNTNVERGKTNAFAFLKSKHAKVPLFILLVLANCSFAYAG
                     SDDGAFGDIWAYMSEALTGAPGKIIACGMLFSVAYFGVVKPNLGLALVSALMMLVMAN
                     GEKIISSFLDAGIPL"
     gene            38336..38686
                     /locus_tag="QBF46_001259"
     CDS             38336..38686
                     /locus_tag="QBF46_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDQ6673463.1"
                     /translation="MRGIFPLCGVFMTSQYEIPPHTYRFPYRINMPLLILFWDAKQLG
                     ITFVTIASGNIFDFFITSVVVAVVFWFAYKKAAEEGIRGKLKHKLWWYGFFPGKSVFS
                     SRYFTDPFIRNLYS"
     gene            38698..39483
                     /locus_tag="QBF46_001260"
     CDS             38698..39483
                     /locus_tag="QBF46_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HDQ6673464.1"
                     /translation="MKLLSRLKIPFIKNSSNGDFDNKDETSQKGGEEVKGSFLDSKAR
                     FSKEIEGSEIGITYSALIKRDEKLLRVNTVAMIIIGVLVVKNQFLTDPVTIVLPPNMT
                     EEVKVVGNKASESYKTQWALFFSTLIGNINPTNIGFVTTTILDALSPDLQAKTRESLQ
                     QQTNIMQARGVEQSFKPIDMYYDTKNDMVYVWGTKSTRLINVPDKTESSKWTFEWVLG
                     MKNGRPRIAYVNQYSGTPNIKKITINGKEQLATLDNPPPSIGN"
     gene            39486..40718
                     /locus_tag="QBF46_001261"
     CDS             39486..40718
                     /locus_tag="QBF46_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDQ6673465.1"
                     /translation="MTSKKVFHCTAITAVILLATSNVVFAEDYQLPATVNNPVVMPVG
                     ADEFQNGVKNAIIKDTGTTTETVQATKPTTALPSLSPASSASPAVNEITGALSNNPTL
                     AGYQQQVKSGDFDSYGRPAGKEPQQNAQSTGSPSKADELYVEARNRYKEVQRVNVPPG
                     GNIVLPVSRGLQNRISTSFKNASVSTSTPADEASIFVNGGDVFISTNTDKPIGIMLSE
                     DSVPESTYNLTLVPLDVPGAMISVTTSLSPTMQAKRETSLDKQNYDEMLARSQSEELT
                     PSDPRQDDHKQRIIDLLTPVALGEVPSGFSLQEDRLSRIPSSEQSPCNFNMYAKLGQR
                     LVGSRELIDVVLVKNDKPYGQVVADQQCITEGVVASALFDKAFLQPGEETELYIVRDK
                     LFKERQTRVTTRPSLIKR"
     gene            40720..41160
                     /locus_tag="QBF46_001262"
     CDS             40720..41160
                     /locus_tag="QBF46_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673466.1"
                     /translation="MRKATFLTVISFGLVVFLGTSYYYQTKYIPKGTVNIFPDVHRQG
                     EIVDDAFEKSTIVISDPTLADKFVGSSTESVGRHEVNVIKELGTQNNGSKFVHDESDK
                     SGLTVGDKWPRAGEPYIVPQMTEYERNLKVKRFQQPKNGVNNGH"
     gene            41150..42508
                     /gene="trhB"
                     /locus_tag="QBF46_001263"
     CDS             41150..42508
                     /gene="trhB"
                     /locus_tag="QBF46_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhB"
                     /protein_id="HDQ6673467.1"
                     /translation="MDIKKAWENKTVRISVIGAALMVLIVVISQSIFTTPVKKEKKTQ
                     KKDMQTGFLIDDSQMNKLSNEESQKTYNEMVRQNRIDQNAAKADRDKAEKAQQENKAQ
                     IASLASQVQQLTTQLTEMQTSRNGNRNLDAGGPRNNVNEQAPAKPYQLNPNAAVNGVS
                     SGYAPISPTRNSPMRTITQSSIKTNGTDGVIQVMPISENRIREGREVVAGSEKAPTRT
                     IRGDGTAPVDSKARHAARKDEMFLPATSIITGVLITGLEAPTSLSSKAEPMPVTMRIK
                     KDIIMPNNYTMDLRDCNLLGSAVGDLASQRAYIRATSISCVNSKGKAFDVIVEAYAVS
                     ENDGKNGIRGNLISRNGNAIAGSAFAGGLSALAGSLSPSKVSSLNIDPNSTAQYQSPN
                     IGALGALAGAGAAQGGLNRLVDYYTSIAEQQWPIVEISPGRPITFVVQKGATIPTNLT
                     SR"
     gene            42517..43029
                     /locus_tag="QBF46_001264"
     CDS             42517..43029
                     /locus_tag="QBF46_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6673468.1"
                     /translation="MENNNDNESPKKSESADSLNIDAAKPVGGIRTTKKVTLEIDIEK
                     TLKYLMVAGLAVLIVIYGYKGGRFVYDNFKAMSQPAYQIAVLDMQTLRKVFNKQNPVL
                     DSQQSKTNFENYFKALMKVYRERGYLVIDASLAVTIPENVEIVSYIDLGGSFGEVQSL
                     PGEPKESEIR"
     gene            43135..43887
                     /gene="htdT"
                     /locus_tag="QBF46_001265"
     CDS             43135..43887
                     /gene="htdT"
                     /locus_tag="QBF46_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide isomerase HtdT"
                     /protein_id="HDQ6673469.1"
                     /translation="MEKAKEGGVSTKPLDGVLAKMKSYKPKDAIYIPTGGLYLFQDER
                     SQLMAVTTDGRYTITGGSVMDILQRKSVLSVEDIRKSFFINLDDAPFPLETVASIPLG
                     NPKLKRQAAIFITLDCDGCQDLIKKFYDEREKYRVDIVLIPSPGEPKQELRQLWCSKE
                     KGKVNDLDILRWLMGNKADIEKRLLTKEEAEACPAEPLVASLMLAGIYKLQGVPSVVR
                     QDGLAGNGIPKDFDYWLKQSVEPLLKNPFDTN"
     gene            43897..44847
                     /locus_tag="QBF46_001266"
     CDS             43897..44847
                     /locus_tag="QBF46_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HDQ6673470.1"
                     /translation="MNIKKVIFSALVVGSSSYLAGCTIGSAESECPGIEKGVICKGPR
                     EVMELTNNRDDLSGLGEEEANSGKGKSAVNDSQYPAQLTPPGAVQYPKSSVLVNKPVT
                     YTTTEVKPVGQLPVMYDQTLKMGAPTSTIGPRPISGNPVNSNVRMTTSYNSNGSSVNP
                     FLHPSAEVVKKTTQVVTPAPAPRYVAPNSDINASKDLYSINNGQPVNPTLSSGQVQQY
                     RTQGYKQAVVAPEPLAVLQQGRVMRITFAPYTDDNDALNLPCFVYVNVKPQTWIAGKN
                     STSNPARIVPLEVQDAARENMQQQQRATKAVSSNGIVRQL"
     gene            44856..47537
                     /gene="trhC"
                     /locus_tag="QBF46_001267"
     CDS             44856..47537
                     /gene="trhC"
                     /locus_tag="QBF46_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer ATPase TrhC"
                     /protein_id="HDQ6673471.1"
                     /translation="MRSANVYNKELSRHQLGGFIPVYDQIPGTHYFLLDGNRLGFMFI
                     CSPSPGVFDNQQDVLTELFKMDFPADTICQTSLTALPDLILHLSAWSAVRGGRMEGHD
                     KLKGDLLTAYQLDYYDRSLNEPLKPDHDKLMLRDFQVWISFSIPLKSALPSEIEKTRI
                     DALYSDLISKLNTVGLFPHKVGAENWLYCMDKLLHPGKTSRWSEGHVEASTMRRLNEQ
                     INVPGRKYTVTENHFSSTTQSNDISEHRYFKQLSVVKFPEFVNFGCMYELVVNWLNGR
                     KTIFSPFMITQTVHFADPLKLSRENVRYKAITNKQASIPTVLTFCPRLKDMDNDYMTI
                     TRELEDGARLLHSYLTFTVMGNSAVDVQSAADQLKSFYLESRVNVADDSYIVFPSFVS
                     SLPMCNDPKTILELDRFEVVSNTGAAHMTPIFGPWKGNTDRPVLNLVSREGQLLGLDI
                     FKTSASYNMVVGATSGAGKSFWVAYIINNYLGAGPRSNNLIHYRDTFEGFKNNSYDAF
                     DPDGAQIFVVDVGRSYQGISEQYTNSQFIDFGKKPDFTLNPFAFLTDVTVGERVFDEA
                     PVFNDDSNNHDDDKDKVAQTIMVLNQLKIMASEKGNIDDFQQSVMLQLISEEYNESRK
                     VGRTGSITGFARRCSNHEDKRIKDIGDQLGQWCEGGIYGNRFTENLPPINFDSRFIVL
                     ELEELKGTPHLQTVVLMSIIQAAQHAMFIKKDGRRRLFILDEAWEYIRPDNASGSSNH
                     SNQFFSSFLEAAWRRFRKTNCAGICITQSFEDYFTSSVGRALTANSPWKIIMKQEKES
                     IEAMKVNNYFSTTDAEYERMKNIRTVKRAFSEMLVRFENFQEICRLYVDRKMELCFTT
                     DSTDRGKLWEIQSRLDCSYGEAIEILYEQEVASKSAA"
     gene            48167..48472
                     /locus_tag="QBF46_001268"
     CDS             48167..48472
                     /locus_tag="QBF46_001268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673472.1"
                     /translation="MNRLTMEGSWLRTVAFNPLNRQTMGGSWSGTVAFNPLNRQTMEE
                     SWLRTVAFNPLNRQTMGGSWSGTVAFNPLIGRPWRDYCRVPGHLTTYSSVHDGMLVA"
     gene            49219..49430
                     /locus_tag="QBF46_001269"
                     /pseudo
     CDS             49219..49430
                     /locus_tag="QBF46_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046583.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        13144 a        11370 c        11781 g        13135 t
ORIGIN      
        1 tttaacaaac agcaggtgca tcagacgact ggtggcatcg tcgacatata caagtgccgt
       61 acaaggccga ccccggtttt caaaccagtg atggtcacag ccgtcaatct gtataagctc
      121 gccagtacag ggccgccggt aacggggctg atgaattttc ggtgcgcgtt gtcttcgggg
      181 gatccagagt ccggctttta tcataaggcg tctgatggtt tccttgccca ggaccagacc
      241 gtgaacttcc tccagttttt cccgggccag cgtggggcca aaatcgcggt agcgttctcg
      301 tatgatactc agtgcctggt cagtcagtga tgtgggcaac aggcggttgc cagtgcgacc
      361 gcgactctgg ttgttcatac caagcggccc ggactggcga taacgcttca gcagacggct
      421 gcaatgacgt ggcgtgatgc cgagcatctc ggcagcctgg ccaggacgca ggttacgttc
      481 aacgacatcc tgaagaattt tgaggcggtt gatttctttc acggtgaaca tcccggcgtc
      541 atgtatagcc atagaaaact cctcatcagg aaaagcgagt cgttgactag gcatacaggt
      601 ttgaaaacta tagcctggac attcctaatg agccacaaat agacattagc attgagcctc
      661 tacacttagt gcgtataaga tggattatgt taaatatggt gggtttgctc ttaagttcat
      721 aagacactcc cactaactga taaatcaata cgttaggatt tctgatctcc cctctcgttt
      781 cttgcattag gtacagagta cattctcttc atgttgttct gaggtgagca tatcacctct
      841 gttcaggtgg ccaaattcag tgtgccactt cagtattgat cggaaggtcg ttagtgaaca
      901 ctcgcacgaa gcgaaataat ttgcactatt ttgaaaagcc aacctgtcgg tttttttgtt
      961 atgcgcgcca ttcgccactc agcgctttct tctgacagca tgttgcttta atgtaaaata
     1021 tactcagcct tctccggaga atcgaaatgg aacttcgcca gttacgttat tttgtccgta
     1081 ttgtggaaac cggcagtatg ggcagcgcgg cgcttgatct cgacatcggt gtctcagcgc
     1141 tcagtcaaca gatgacaagg ctggaaaatg aactcgccat tcgtctgttg caacgaacct
     1201 cgcgtggtgt aacgccaact aactcgggtc tggcctttta ctcacaggca cagctggcgt
     1261 tgcgtcatgc tgatgatgcc attcttgctg cgcgcgatgc gcgtctgtcc ggacacgtca
     1321 gcgtcggtat ggcccccagt acagcctccg tcctgggtgt gccgtttatt catgccatgc
     1381 gggcacatta cgccgacgtt cgcctgcatg tggtggaaag cctgtcaggt aatctggaac
     1441 ggatgatcaa cacccgtcag attgatctgg ccgttgtttt tcggaaagaa aagcacctgc
     1501 gctggagcgc caggccagtt ctcgaagagc agctttttct gattggcaca catgatctgc
     1561 tgacggatat tccggatgac accataaccc ctgctcaact ggcgaatatt cccctgatca
     1621 tgccaagctc aggccacggc ttgcgcggcc tactggaggc tattagtcag gaacacgggc
     1681 tgaaggttga tatcgccacg gaaattgacg gcctggcgtt actgatgcgc gccgttggca
     1741 gcggactcgg cgcaacatta caacccggag cagccatttc gcatcttgag agtgaatcat
     1801 taagagtgat cggcatccat aatcccatac tcagtcgccc caattttctg gtcagcctat
     1861 cggacgatga actcccaccc gccgctcttg cagcccgcgt gatacttaca aaagtcatgc
     1921 gtaaacttgt tgaaatcggc cattggccgg gcgcaaccat ttacgctccc taaacccccc
     1981 tttagttatt cgttatagcg cttacacaac acataaatat acacttcatt tacaaattta
     2041 acagacagtt aaaaggtagt ggagtagacg atggttgatg tactggtgat tggcggtggt
     2101 aatgccgcgc tatgcgccgc cttaaccgct cgtgaggcag gagcttccgt gctgctgtta
     2161 gaggctgcgc ctagggaatg gcgcggcggg aattcccaac acacgcgcaa tttacgctgt
     2221 atgcacgatg caccacagga tgtgctggtc gagagttatc ccgaagagga attctggcag
     2281 gatctctggc gtgtcaccga aggcaatacc aatgaagcgt tggcgcgctt ggtgattcga
     2341 acctcttccc agtgtcgtga ctggatgcgt aagcatgggg ttaattttca gcctccgctt
     2401 tccggcgcgc tgcatgtggc gcgcaccaat gccttcttca tgggcggtgg taaagccctc
     2461 gtgaacgcct attaccgcag tgcagaaaag ctgggggtac agattcgcta cagcacgccg
     2521 gtacaggcgc ttgaattgca caacggcaaa ttcgtcgcag ccctggcagg tcacgaacgc
     2581 attgaggcga aagcctgcgt actcgccgcc ggcgggtttg aatccaaccg tgaatggcta
     2641 cgcgacgcct ggggagagaa cactcgtggc gaatggccag ccgataattt ccttatccgc
     2701 ggcacccgct ttaatcaggg cgtcttgctc aaatttatga tcaatgccgg ggcagacatt
     2761 atcggtgatc cgtctcagtc ccactgtgtg gcgatcgacg cacgggcacc gttgtacgac
     2821 ggcggaattt gtacccgcgt ggattgtgtc tcactgggtg tggtagttaa ccgggatgca
     2881 gaacgctttt acgatgaagg ggaagatttt tggccgaaac gctatgccat ctggggacga
     2941 ctggttgccc accaaccggg gcagattggc tactccatta tcgacagcaa agcgatcggt
     3001 cactttatgc cgccagtttt ccccggcgca caggccaata cgctaagaga tctggcccgc
     3061 cagttggggc tggatcccga acactttacc cacaccgtgg aacactacaa tcaggcctgt
     3121 cagctgggtc agtttgatca cactaagctg gacaactgcg cgacgcaggg cctgacgcca
     3181 ccgaaaactc actgggcacg gcctattgat acccccccct actacggtta tgccctgcga
     3241 ccagggatca cctttaccta tctcggactg aaagtgaatg aacacgctgc ggtccatttc
     3301 gcaggcacgc ccagctgtaa ccttttcgtt gccggtgaaa tgatggctgg caacgtactg
     3361 ggcaagggct ataccgccgg ggtcggcatg tccattggca ccacgtttgg tcgcatcgca
     3421 ggtaaagaag ccgcgcaggc agcacgcaag gaggcgcatc atgaagcagc ttgaaaagtt
     3481 gattatcgag gccaaaattc tcaccgaacc agaggctgaa gtcgagcggg tgatgcaggt
     3541 ttgtaacgca tgtcgctact gtgaaggatt ttgcgccgtg ttcccggcaa tgactcaacg
     3601 actggaattt ggcaaagccg acattaacta tctggcaaac ctgtgccata actgcggcgc
     3661 ttgcctgcac gcctgccagt atgctccacc ccatgagttt gcgatcaacg tgccgaaagc
     3721 aatggcggaa gtgcgtctgg aaacgtatca acactacgcg caaccggcgg ccttcggagc
     3781 gctgtatcgc caggctggcg ttaccgtgac gctggcactg gtctttagtc tgatcctctt
     3841 tttactcctc gcgatggggc taaagggatc gctactccat ccgccgctgg ccggtgattt
     3901 ctaccaaatc ttcccgcaca acctgctggc atggatgttt ggatcggttt tcatgctggc
     3961 gatcggttta ctaatggcag gggtgatccg cttctggcgc gagatctctc ccgtcgggcc
     4021 gcgtccggcg gaaattgccg aagcgtcgca caacgcgctg acgctgaaat acctcgacgg
     4081 cggacatgga aaaggctgca acgaggcgga cgatgctttc acattgatgc gtcgtcgttt
     4141 ccaccatttc accttctacg gctttatgct ctgtttcgcc gcgaccgtgg tggcgacggg
     4201 ttaccactac ttcgccggct gggaagcccc ctacccgttc ttcagcgtgc cggtcatgct
     4261 tggcacgctc ggaggaatcg gtttaattgt cggcccggca ggcctgctgt ggttaaacct
     4321 taagcgctcg tcactgcatg gcgacgcgcg acagaaaccg atggatcgcg gttttatttt
     4381 actgctgctc ctgaccagcc tgaccggtat aggtttgctg gcgggaagag acacctcatg
     4441 gatggggatc ctgcttgcca tccatcttgg tgtggtcatg gcgctttttt tgaccattcc
     4501 atacggaaaa tttgcccacg gattttaccg ctgcgcgtct ttactcaaat gggcgattga
     4561 gaagcgacgt ggaaaacagg cgggggtcgc aggcgactaa cccgcaaact cttacctcta
     4621 tacataatta tgataagaca gtggagcaac ttcaatgact caacaaccat cgcgcgctgg
     4681 cacgttcggc gcaatactgc gtgtgaccag cggtaatttt ctcgaacagt tcgatttttt
     4741 cctgtttggt ttttacgcca cttatatcgc aaagacgttt tttcctgccg aaagtgagtt
     4801 tgccgcatta atgcttactt ttgccgtttt tggctccgga tttttaatgc gccctatcgg
     4861 ggccgtggtg ttgggcgcat atatcgacag gatcggccga cggaaagggc tgatgatcac
     4921 cctggcgatt atgggctgcg gcacgttgct catcgccctc gtgccgggtt atcagactat
     4981 cggcctgctg gctcccgtgc tggtcctggt ggggcgtctg ctacagggat tttctgctgg
     5041 cgtcgaactg ggcggcgtct ctgtttatct ttctgagatc gccacgccag gcaataaagg
     5101 cttttacacc agttggcaat cggccagcca gcaggtggcg attgtcgtcg cagcgctgat
     5161 cggctacggt ctgaatgtca cgctgggaca cgatgaaatc tccgaatggg gttggcgaat
     5221 cccgttcttc attggctgca tgattattcc gctgattttt gttcttcgac gttcgctaca
     5281 ggaaactgaa gcctttttac aacgtaaaca ccgtccggac acccgggaaa tttttaccac
     5341 cattgctaaa aactggcgca tcatcaccgc gggtaccctg ctggtggcga tgacgaccac
     5401 gaccttttat tttattactg tctatacccc cacttacggg agaacggtgt tgaacctgag
     5461 cgcacgtgac agcctagtag tcaccatgct ggtagggatt tctaacttca tctggctgcc
     5521 gattggcggc gctatctctg atcggatcgg gcgacgcccg gtgctgatgg gcatcacctt
     5581 gctcgccctc gtcaccacct tgccggtgat gaactggctt accgctgccc ctgacttcac
     5641 ccgcatgacg ctggtgctgc tctggttctc gttctttttt ggcatgtata acggcgcgat
     5701 ggtggcggcc ctgactgaag tgatgccggt ttatgtccga actgtgggtt tttcgctggc
     5761 ctttagtctc gccacggcga ttttcggcgg cctgacacca gccatctcca ccgcattggt
     5821 ccagttgact ggagataaaa gttctcctgg atggtggcta atgtgcgctg cgctgtgcgg
     5881 actcgctgcg acgacaatgc tgtttgctcg tctcagtagt ggttatcaga cagtagagaa
     5941 taagctctga aacgcaagca ggcagagaaa tatctctgcc tgctttctcc agtgcgccgt
     6001 aaaaccgtgg ccaccgtgac acgggagtta cgtaaccggg ctatatgcca acacttcagt
     6061 gagttcgccg gtgcatcgat gactggttat gccagcaatg ttcagaggga agaataataa
     6121 ttgcgcaggt aacgtaatat tctcttcaga gatatatcat ttatgaatga ataagctaag
     6181 tactaccgcc tccggatgtg gataaacatg ttctgtggcc ctgacaaagc ctcagaagcg
     6241 ccccacggca ttttcagcag tttacagtac ccggggtatc actatgagcc gtttaagcca
     6301 gcaccgggta atcggctaaa tccaaattta ctcgatttag ttaccgatgg tttcgcctcg
     6361 aagatctgca tccctgactc tacgttgtca tggtattcga caggaaaact ataatgatgc
     6421 atctatagtt gcgattttaa actcaactca gttaatgact ctcatcgtct caccgaatct
     6481 tctatctgag ctgaaaaatc tatccattca ggaaggatac atgctaaaaa tagaaaatgt
     6541 actgccgcca aattttagag tattcctggt ccgcgaaggt ttcattttcc cgggcgaatc
     6601 atgggtgtgt ggttccgggt actggttgcc tgaaagtgaa gtactcaaaa acggaatcaa
     6661 ctcattgctt gtttaattag ccgttccatc ataccggaga cattctttca tgactgacca
     6721 ggcctctatt cccgttgata ctccagttct ggcactcgca actgatgctt attcatcact
     6781 aaaaaatatc ctcaatgaca atgggacaag cgacaccaca ggaacatgca tgttcgccag
     6841 tctgctggtt tgtgaatttg cgcaccgcag aggtatgtcc gcagctgtgc gaggcgggaa
     6901 cggaacagat gatggtggta ttttcaatga gtctggcggg cacggccatt actggtgtga
     6961 ggtaagtgcc ggtgaaatga ttttttatat cgatattgcg gccgagcagt tcggctaccc
     7021 ttcgtttatc atcaaaaatg caaatgacgt gtcagggtgg ccgcgatata tccccggcga
     7081 ccaggttaca gtagatgaac acgtcaggat tacgttgtct ggaggcatta gatagtccag
     7141 ccttgtttaa ccagtgaagc ctagaatatt gagattcagc caggaataag gctgacaaca
     7201 tcataattac tgttaattaa atgatagaca tttagagtca aacatccaag atttaaaagc
     7261 ggcgtcacag aactattaag gaatgaattt ttttaccctt aaacaaaacc accgcttcag
     7321 aatggatagg cgaatcttac attacaggag tttttttcca gctgccaatt tgttcatgaa
     7381 tctgcctttc aattctttcc acatgagcta atagctcagt gtaaccaggc agattggcgc
     7441 caatatcacc ttcccactga cgggttagca ggcggacttt atcttttagt gttgaaagct
     7501 gagtgagttg ctttttggca atcacaatct cttcagagta taaattcttc tttggcatac
     7561 attataaaat ttaacttagg taatgaagcc taccttttaa cacaacctcg ctttattgca
     7621 acatttaatt acatttacat tccacaggaa ctataaattg accaatatcc agcactgctc
     7681 ccgtgattta ggttagcatc attcccggag tgcagaggga tcttgcagag tttccgtaag
     7741 acgcatttgc aggtcagcga cctcatcctg aatgcctttg agctccgact gacggataat
     7801 ttcatggtcg ggctgatatt caaacaacgc gaggaaatcg gccgtcagaa accgatgctc
     7861 attcagtctc ttgtctttcg ttgattttga accgttctca gcgacgattt caacgcttac
     7921 ccattccccg taatgccggg ggtgagcgcg aaggatgacg gtatgcagat tgttatccac
     7981 gtaccgcaac gagttaatga ggagagtgtc cccggccaca atcgcctcag ccggaatatc
     8041 gacgagcgca gaccaatact gccctgccag cagagaatga aatcgttcaa ggcgatcgat
     8101 attattttta ctgaccaggt cccccataaa ctataccttc cgaatttgtt tgttataaac
     8161 atcataccat attggctgtt cacccaaata acctggcatg cggaaacagg tgattcattt
     8221 aaaacagagc ctgtccattt taaatggtgg aatccagcaa attaaataca gccattatca
     8281 aacgagttga ggattaaagc atgtattgca ggagtatgga tttaattgcc cattaagcca
     8341 cgcgccttta aagacataga cctaatgagc gttttgtatt ataaatctca cgcaaattta
     8401 tgttatttat ttggctgaac tatttcccag tatctttcat aaacactgaa ctggcattta
     8461 gatatgtcat ctgaagagga tcttgttcgt gtaacaaatg cttctattgt atcttctgat
     8521 tgtttagata gtttatcacc tggttgtttg tgaagaatca ttagtaatgt ttcagtagct
     8581 ttctttctca tctccactgg atcagttaaa ccaccagcat ctatgcacat gtttgaagta
     8641 actgcgtcgt accctaaata aggtattggg tcccttttta tattctgctt atcgctggca
     8701 tgaacagaaa ataccaatga aattattcct gccaatacaa tgtttttaat tttcatatgt
     8761 ttctccatta ctatcaccaa gttcaaaaca taatttttat atcacaaatc tttctaagca
     8821 ataaacagct aattcctgtt gtgaagcatc atttttcatc ccggcgtaca aatgacaccg
     8881 ggaacagaat ccgtcaggaa ttttcaaggc atagataaat tgttatcaca ccaatattga
     8941 tgcaggcctg caatccatac tttcgagact gccgacagcg tctgcgccag atcacgcata
     9001 cattcaggtg tgaataaccg gactggctcc cccataccgt cactcgcagt gatgatgtgc
     9061 tccaggatgt tcccgtgaca taaattgtgc cgaaccctca cgatttccgc atagggtagt
     9121 ttcggggcgc cgtccgcaat cttggtcagc agatcctgct cgtcgggaaa ggccagcagt
     9181 gtcaccggca taccggcctg gtgagccctt cttagcagag cattactgag tgtggccata
     9241 tcgcttaagt ccaccagcgg tgttggtgcc tgcggttgtg cctgcgacgc cttcagcttc
     9301 atcgtcaccc tcagtgaggt ctcaatcccg ttcaggaaag tcgtacaggc ggctagccac
     9361 aactcaccgt gaatggcccg ggtagcctgg tcaaagaacc agtcaatcgc agctgtcgtt
     9421 cggtacgccc tgtatgcctc cagttcccgt aactgtgcca ggtacttctc gcacgcctct
     9481 gcgtctaccg gatcgttatc gtcggtatcg atagccatag ggaattcctg ttgtcatgtt
     9541 cgtgaatgaa aatccgctga accatatcat accgttacat aaattacggc ttagtgccgg
     9601 gatatctttc ccgaatgctg agccattgaa atttgatctt ttctctttat ctggtaatac
     9661 tggttaaata tccagtattg aatccgagtg tgctcatgtc tgtattccct gaacgttttg
     9721 catcccctcc tcgtgataca accccttttt tcagtgacct actgagttgc ggggtgatga
     9781 gtccgtgcgc cggacacgaa gacaatgagc tgaatttaca tgattttgtg gttcgtaatc
     9841 gccccgcgac tttctttgtc agggcctcag gtctcagcat ggtagacgcc ggaataaacg
     9901 acggcgccat actggttgtg gatcggtcac tgactgcgga gcacggctca atcgtcgttg
     9961 cgctggttga cggcgagttt accgtgaaga ttttgcagac cttcccgaaa ctgatgttga
    10021 tgccctccaa ccccgccttc aagccgatac ctgttaaccc ggacactctt gaaatctggg
    10081 gcgttgtcac ctttgcgttg aatcagtttc ctcatgtaca tgcacgttga tatcaatgga
    10141 gcctacgcgg catttgaatg cgccatggac cccaaacttg ccaaatcccc gctcatcatc
    10201 gcgagcaaca acgatgccac cattatcgcc atgaacaggc tcgccaaaaa tacagggctc
    10261 aaacgcggga tgcctatctt caaatgccgg gatctgattg agcagcatca tattgcggta
    10321 cggagctcca actttacgtt gtacgaagat tattccaacc ggtttcatga aacgctggag
    10381 gggtttgccg aggataccga tcgttacagc gtggatgaaa gcttcatgct actgaaaaac
    10441 atggacaaaa tagtagattt tgaagagtac ggatgcctga tcagacgaac gctgctacac
    10501 aatctttcgc tcacctgcgg gattggagta tcgaccacga aaacgctcgc aaaattatgc
    10561 acctacgcca gtaagcgatg ggcagcaact ggtggtgtgg tcgtccttac agacccattc
    10621 cgaattaaaa aactgctgag tctcatcagt acccgggaaa tctggggcat tggccgcaaa
    10681 atcagtgagc ggctggctgc gtacgggatc atcacggcct gcgactttta tgacagcgat
    10741 gtgcggtttt tacgaaaggc attcggtgtt gaaatggaac gtacctggcg ggagctgcat
    10801 ggcgagtcct gtttccggtt gcatgaatcc cctcctgtca gacaacaaat tatcgtctca
    10861 cggagctttg gccagcgcct taatacattc agcaagatgc atgaggcagt gtcattcttt
    10921 accggccgtg ccgctgaaca gcttcgaaac gatggctcct ggactcgcca aatcaccgtg
    10981 tttatccaga gcagcccgtt tgccagagct gaaacccggt acagcagctg cggtattgag
    11041 ccgcttactg cgacgcagga cacccgcgac ctggtcagtg cggcaggcgc catactgacc
    11101 cgaatctaca aacccggcat agattatgcg aaggcgggcg tgatgctttc gtcaatgacc
    11161 gatggcactg agcagctatc cctgttcgat gaacgtcccg ccaggcgagg cagtgaagca
    11221 ctgatgaagg tgatggatcg gtttaacaaa gagcatcgag gttctctgtt tctggcttgc
    11281 gaagggattc atcaggattt ccgggggaag caggccatgc tgtccccacg gtataccact
    11341 cgctggaatg agttacttgt cgtcaaagcc tgacgttcat gccggtgtta cccggcatgg
    11401 ccagtttcgg aaggaaaagt aactttagcc tccacgggcc ttctggattg cgtatttcag
    11461 ggtggcctcg tcaacagcgc ccgggatgaa tgacgtcgtt gcagcgtcgg gtttttgcat
    11521 gttcataata atgaaccctg gcgtaccgtt aatcccgaga gcttccccca gctgcatgtt
    11581 tccggagata acgttctcat agcggctttt ctcccgatcg ccgaatgagc tgttgaaccc
    11641 ggatttgttc acaaccatgt ctaaatcatt gagtgtaaac gcacgctggt tgttgaagaa
    11701 tacatacgca ctggtcatca gattgttgtg atacttacga tacgcttccg caccgaatgt
    11761 ctgatacaca tgcaggcctg tcgccgcccc cattgcggaa acaggttttg agccagcgaa
    11821 gatcggaaat tctttaaaga agaactttac atcagtgctt tggctcaaca cagattcaac
    11881 aaccggcgct acctgcatac agaaatgaca ctggtagtcg aaaaactcaa taacagcgac
    11941 ggccgcatta tcaggtccga tattgggtgt ctctttggtc tcgaagagtg ccggtgcgta
    12001 ggggataatc cgctcaaggg aagcgttagt attttctttt tctagcgcct tacctgcttc
    12061 gagaagatac tggggatttt ttaccagata ggaagcagct actcgtccta tttcgtcgtt
    12121 acccggtaca tcgggtttta gatatttcga cgtaatatac acagagccag ccgtcgcaat
    12181 caccgcagaa attaaaacag acgccacggg ttttaaataa tttaatttaa tcatagccac
    12241 ctttacgcat ttcaacttgt tagttatcag cctgcgttcg attatactgg cacggtatat
    12301 cggaattatt cattaaagaa ccctttttgg aggatttaga atgttacggc aatttttctt
    12361 actaacagcc acaacgttct tatgtggatg cggttatcat tttggaaatg acgttgatgc
    12421 gtacgattta ctgccccgcc cggtaggcag caagaaattt gaaattgttg ctccggatga
    12481 cagtattcag tcgcgaatgt ttgcagcccg gtttgcgagc ggattaacgg gtaaggggtt
    12541 taatatttcc agccatcagc ccgaatatat tctcagattc agttacagca agacccagga
    12601 gaatctgcaa tacagtgaac taccggtgac gggaataacc ggatatgtta tcgagaagaa
    12661 aacaacgcga aaagataagc acgggcaaac tgagacggac tatgactata agccggtcag
    12721 tgggatcgta ggaacagaga cggtatcaca aagacatttc tggcgtcgtc ttgatgtgga
    12781 agtctaccct gcgggaaaaa atgcacagca ggtgctaaaa gtcagtatgc agagcaatgc
    12841 gccgatccca tcggacagcg tggcgtattc cgcaatgatt gacgctttga ccgggaatct
    12901 ggatgccccg ctgcgctccg gaaactacgt cgcctcggtc ccctggaact gatgaccggc
    12961 actgtaacga ccaaccacaa attaactttt ctcacctgca tgccacgtct ttaccggcct
    13021 cctctgttga aatcatagag tcggtacaga tcaccgatgg tcagctcatc cactttggtc
    13081 acgaagagat attcaaactg agctttctga atatctgaga ctgacgcatc gtccagcacc
    13141 gtttggtaaa atttggcatt gatacatctt tccggatgca tgaactggta ccgcccctgg
    13201 agcaccacca tcagaaagat caatatcagc agaacgagaa gtaacatctg gttggaaaga
    13261 tccagtccca gcgacacacc aatggttgcc atgaaacaga gagtccatcc caccagggcg
    13321 taaggagcct tttggatctt ttgttggtaa taacggagga tcaccggatc atttaaatac
    13381 cgcttatccg gcaatcccgt tgcccgggtg acaatgctga gccaaagcga catcttctaa
    13441 cccccagcga cgcactcagg ccagacgttc atagaacgca acaatgggct ggaagatggg
    13501 tgtttgtacc agctcggggc gaatggcttt cagctgcctt accggcgcca actcatccct
    13561 cggcttagcg atttcattgc tagggccttt cttggtactg tcggtgttca cctttaacct
    13621 gtcattgctc agatacgact caaaccgttt cgacatcacg tcgctgcggg atgtcgcatc
    13681 attgagtttt tttaaaactg cgtctttatc tgtctgagca gcagatacca gactctcact
    13741 gctggccaca gtgtcggcgt cgcaggcccc gaactggcat ttggcagcaa agacgggttg
    13801 agaaccaacc gccaccagca ttaacgttgt cagtattgca gatttcataa acacctcttg
    13861 cagtcgtgga aagtcacttt tgttcggcac ccatgcgggt caccagattt tccgggctca
    13921 tccaaccggt gaatagctga ggtttccccg acagcgttac catcgttggc gtaccctgaa
    13981 gcggaatagt tgaagctacc tggaaatgcg atttgatggt atcgaggcag gcctcgtcag
    14041 gccgtccttc cggaatggtt gatgtcgggc tcattgcggc atccagtgca tcattaggtt
    14101 ttgccgcaca ccatattttt gccatattcc ccgcgacaac ggagttgaga cctgcacgtg
    14161 gaaaggcgag gaatttgatt gagatcccgg cgtcgagata ggattgaaga tcttgatgca
    14221 atttctggca aaagccgcat gtaatatccg taaacactgc cagacggtat ttttcattcg
    14281 ccgacttata ttcgatggat tttgttttag tggcgaactc cctgacacct ttcagaatag
    14341 cctgctcagt tgtgttctcc acatctttcc cgttcacatg aaacaagctc ccggtgaaaa
    14401 tgtaatcgcc atctttgctg acgtagctca cgccctctct tgaaatgacg gtataaatat
    14461 cttttaccgg cgatggttca atatgctcga tagacaagcc cacttctctt aatttcttca
    14521 gtgcagcatc gggaagcacg tcagtgttcg ctgtctgggc attcatactc aaggccgtac
    14581 aggatgccag taaaaataat gatgccttga tagtcttatt catttcagct cctttaatgg
    14641 agtgttaata ctatcatgcg aaaaaatctg cctaattcgc ttaaggggaa taattccccc
    14701 ctatagcaaa aaaaattttg acgacgatat gggagaatat ccagaacgta attccagcgg
    14761 agattatctc gtgtttatac caccagtaga cgatgtgaaa cctattcccg ttcctgttga
    14821 gatctacacg cagtgcatta ccgatgcctc ccgttttttc gggattgatg cggagttggt
    14881 ctttacgctt tttgataatg agggcggaaa agtcgggacg ttcagccgta ataaaaatgg
    14941 cacttatgat attgggccta tgcagatcaa ttcgtccaac ctgccagaaa tacgcgacca
    15001 ttttccgtct gttacgtggc gggtgctggc ctatgacgcc tgtgccagtt tctgggtggg
    15061 cacctggtgg ctttaccgga agattgttga ccggaagggt aacgtatttg aagggattgc
    15121 cgattacaac agcaaaaccc ctaaggtccg ggcgacgtat atcttcaact tcatgatcaa
    15181 atacaatcgc cggattcagc gccgaaatgg aatggacgaa ttatatcagt ggacgcaacc
    15241 taagccccag tacaacgggc atattgtaaa aaatcttcct gaataaaata cgaccctact
    15301 ctccaatttt ttattagttg tttcgtagcc ataaacaatg cggctcttag agaatgaaaa
    15361 cgtcctgaga aaaaggttaa cgatgctcca gtcaatttat aaattcctgt tcaatttatg
    15421 cagttcaccg cagctgccca aaaataaaga cgtggatggg atattgtctc atgaagaaat
    15481 aaccgctgga ttatggggtc ctctgccatc aatccatgtt gttcttttcc tggattttga
    15541 tggtgtagtc cacaaatgtc agaatgaatc gttcgagagg atgcatttga ttgagaggtt
    15601 acttgacgcg agtccttcaa tgttcatcgt tatatcaagc tcatggagag agtgcggcag
    15661 tatcacttac ctaaagtcac ttttccgctc gccatacaga gacagggtaa ttggagcaac
    15721 cccatccctg tatctcccct caggaagtat aggagtccgt gcagctgaat gtgaggactt
    15781 cgtcaccaga cacaaagtaa aagcctttat ctgccttgat gacgatgcga ccctgtttcc
    15841 tccagagtac ccgcacctgt ttcgtacaga ttattacacc ggggttaatg aaaaggatct
    15901 aacggacctg atagccagat ataatgtgct gatgacccgc ttttattgaa aaagtaactt
    15961 ttccacaccc tccatacaac caaagcctct gatgcgctat agcctaggca ctaaagcatc
    16021 agtcgattca gaaaagttac ttttctctca attttcattt tgaacagaag aaatgcacat
    16081 tttcttagtg tgccgggtga cctgtatttg gtgaggaaat ttgaatcagg gtgatgcaag
    16141 caaaaaaaga ccgtactcaa aagagagtac ggcctttatc agctactact tacttttact
    16201 tcttattttc attcagccag cttttagctt tctcaattct caactgatat ggttcatttg
    16261 tattattcag gtttgacttc ataaagctca cgaatgcatc tgctttatcc ggcccaaaac
    16321 gtggatcatc ggtcagttgc tgacgaatcg cacgttctga gttttgtgat gcagtctcat
    16381 acgccttgat cccatccccc cccatatcct tatacctgct ggtcacctcg ctaacaccat
    16441 ctgacatttg caggtgattt ccaaccttgc ccaatagcga gctcggcata ttttgtggat
    16501 tcgaagcatc gtttgccaaa gatgaagggg tggtcaggat acccatacct gagagcatgt
    16561 cgtttacagg tttagctccg ataacgtcaa gtcctctggc aacactctcc tgccccgata
    16621 aaagcgtact accaataaca ccacgttctg tattggactc aattttatta ccaacaagga
    16681 gatccgtcgt ggtttgggtt cgttgcccaa cgagcccgcc gccagaacca ccttccccaa
    16741 gattcgccct cacaactgag tcaagagaac ctgaagcctt gttatgcatg ttgaaacctt
    16801 catggatatt ctggagatcg ttcatccccc agcgaacgtt atagtgactc agttcggaga
    16861 ggatattttg agtatccgct ttcagttgtt ctttctcagc gccggttgcc attgtactgg
    16921 cgccataggt aaacacagct tgtgcggcca gttccgcacg tttgtcatcc ggctttttgg
    16981 taggatcatt tgcaatcctg ccaaaataat tcgggaggtt gcctgatata ccgcgtatct
    17041 ggttagggga cattctacgt ggttttctac gcccccccct tctctcttct gcggctcaca
    17101 gaacttcagg aattgctggt gggcagtctc caggacgttt tccagtaacc ggctgtggtc
    17161 agcgtcatat cccttcagca ggcgttccag ttctgccata gcgagtgcag gtggtaacca
    17221 gatgacgcca agcagcaaaa actggctctg cattcgaatt tcatggcgat ctggcttcag
    17281 accctctgcc cgggaagcgc aataataaat cctctccagg ggagctacac gcgtgataag
    17341 aggtttctca gcaatttctg acatgcgact aaatcagcct gtttcgtttc tttctgatta
    17401 tgttaagaca ctatgaaaaa gccccgtaat acggggccgg ttcggaggca gttactggat
    17461 ttgcggcagc tcgatcatga agcttgattc accgacattg ccgctggtgg ccgggaagat
    17521 ggggatcagg acgccatcac cctgcccgaa atctatcgtg atgtctttcg aatccccggg
    17581 tacgacggcc accccattca tactgaccgc aaaggccgca tctttgcgga ccgtcagcgt
    17641 gccggtttgt gctccagtga tcagcgaacc atcttttttc cggatgctat tggtacgcag
    17701 atacgccagc ggctggttat ctgatgtctt cagtcccatg cccacggcca gcgtcttgat
    17761 ggtattaaat tcaatcaggt tgttcggtac atagcggaac cggctggtct ttgtggtggc
    17821 gtttccagct tcatccatag ctacgacagt caacaggtag gaactcgcgt cgtctgtcgc
    17881 ggggaagata cgcgggtagt tcagtccata tttgtcggtt ccctggctgt accagcctac
    17941 gctgacttta tctgacgtgg gtccgccgct cagcgtaatg ttcttcagag aagctttagt
    18001 catgtcatcg ctcagggtga tcaccaagtt ttccaggcct ttgacaagcg atccgtcagc
    18061 tggttttcct tcaaagatga taccgacgac tggcggcgtc acgtcatgca ggatggtgct
    18121 cgatagcttc agttcagccg tgttgttgta ggtgtccgtc gcagcacccg ttgcagtgta
    18181 tttcccggaa ggtaacttac tgtagtcaaa cgtcacttgc ttcagctggt agttcaggac
    18241 tgcgacgctt ttcggcgaca gggttgtgac tttattactg gtatccttca atgagaggtt
    18301 gaaaatgttg gtggcccaca tataggtggt ccagtcgtta gttacgtcgc tgtcagtcac
    18361 cagcataacg actgttttat ctagtgggtt ggaaacaata ctgttgatga caggaggatt
    18421 catatcccag taggtataga aatgcgactg aagtccacca agattagcgt aagtaccggt
    18481 ccctttcata tgaatttggt acggactata gcctttaccg ctcgtcttac agtaatccat
    18541 gctgactaga caactgctct cccccactgc aacattgcag ctaccaagac ctatgatagt
    18601 ggcggtttgc acatacgggc gtggttcaac gttaacacgg cccatatcaa cgcaatccgg
    18661 tccagtgaac ctgacaatgt cggtcggtgt ccaggaattg ccttttttga aatgaaactg
    18721 cccggataaa taacgtggag cttccggcac accgctgccg agagtaaatt tcagtgtact
    18781 ataattgata ttattgttca gaccagcctt ggtataccgc tcgtagtaaa cataggtctc
    18841 tgggtaactc accgtgactt cagtataaaa gtaggtgctg tcagaattag taatctgatc
    18901 gatccagcca aaatcagtcc cgttaaaatt tgttgagtcc gatttaaggc gtttgaaccg
    18961 gaattttacc gggttttcat aaatggtcat gccaggaaca taatcttccc agacgcctgt
    19021 tttcgcattc cgcacctgat gttgaatgta gctggggacg gtacgatcaa taccagagcg
    19081 atgtgatgtt tctgtccagt tcccggcttt gtcataaacc cgaaaaccta cggtgtaata
    19141 tgccggactg acgggcgcaa ccgtgcttcc tgccgccacc gccgccagtg cctggaccac
    19201 ccccgtgagt gcattcagct gagcatttac agtccggcgg acaccgctgc tgtcaatagc
    19261 aaaccactcc gctttatcca gtcctgacct cgcatcagag acgttcgata acgaaatggc
    19321 ctgggttgcg gtcctgtcgc cgaaaatatc cagacttccg tactgaaaac cagccctgat
    19381 ccactgcatc ggatctggta gaacgggagg agtgatatcg atgctgaaag agtagctggt
    19441 tgtcgccacg acattcgact tattgtcgag tgtttcggat ttaagagaaa aagtaccctc
    19501 gcccagtacc ggcagtgtca tggacttgcc gtagtactca ctaccatcag cggcagtgat
    19561 cctgtcggcc actgtcacca ggccggtgac actctggaac atctgcacat tatcggatga
    19621 acgtgtcacg gtaacgcgct cgtagcggtc gagaccagat atcagattca gcgtaattgc
    19681 gctgcccggg ttgagatact ctctggtggg ttcagcattc actttcgtgc cgcgtgtgtc
    19741 ggtatacgaa tagttgtaaa gctgagcctg gctgtaaagc gggaacagca gtgccgtata
    19801 gacggcgaca atcaacggac gtcggcaaaa tttcatttcg gttctcctgg ttggccagaa
    19861 aattttacct gaatcgtttt cttcgcccgg gcgctaagag gggtaattgt tcagtaaaac
    19921 atcgtccgtt tgtgtgcttt ttgccagatt taatcggcag gcctgcggga aataccccat
    19981 cccaatgtcc gttttaggct gattaattcc ccagaaatcc cccccttaac ggaaaaatcc
    20041 gcagggcaga cgtttaaaat agcaacaact aaaaggggaa tactgaatga aaaatcaggc
    20101 tatagctttg gctatggcta tgctcggagt ctcagttccg gcatctgctc aaatttatga
    20161 atccatcttc acagatacca atggtattga agttcacgcc ccctcaaccc ggctgttgct
    20221 gaatccggcc tcaccggtaa ccctgacgct cattgccggt cttgaccgtt atgtgaatgt
    20281 caaaatcacc agcgccaccg gcacgcaact gctgaacacc actactacgc gcaccagcgt
    20341 atccgaccgt ctgaaggccg gagatggcag cgaattttac ggaaaaaaag taactttacc
    20401 ggtgcttggc gaaggcaggt cggttgtgca ggtcagtatt ctggatctga atcagtccgt
    20461 tgtagccacc tacagctaca actggctggt agacaccaca ccgctgtctg ttaatgccct
    20521 cacaggcaac gccgcctatg gatcaactgc gggtagcgtc tggaaaatgg ggctggaggc
    20581 cacggggcag tttgatttta cctcaaccaa tgtcacggac gtaaatggca tcgagaaagg
    20641 tatgttttat gtctatcgca gtaacggcag tttgttcagt acgacgtgac tggtcagaag
    20701 atgtatcaca cttactcaaa aaactcggtc aaaggcgcgg gtattcccga cagcaatctg
    20761 gatgaagatt tcacggccaa ggtagtcatt tatgacaacg caggaaacac ccgaaccctt
    20821 ccgttgcagc tctttcgata tgataactat ctgggcggtc cgagatccgc aaagctccgc
    20881 cagcgtggtg ccaggggttt ccggatatcc ggcctattca gctgggatga ccgttaatga
    20941 gaatccgatt catcttgtat atcgcgtgcc aaagtccaac taccggtcat atgccgaggg
    21001 cggcctgcta ttctataacc tgtattcttc cccgaccgag attgcgacgg acagcaccta
    21061 tacctacgtt gaaatgatcc tgccctagta cggtggtata cgggctggag aatatccgta
    21121 tagctctggc ggataacctg acaacacccc ggatcacccg tcttcagata accggagggc
    21181 caacggcaga taacgttgag ctcacgtggt cacctgctgg cacgaacgtt tatgcgccag
    21241 aatatccaag gctgttcccc tcattcgagc cttccgaaaa ctatgcgctt agcgtgacgg
    21301 tctccgacag tcagtcgaac agcaaaactt atacccagaa attcagctac ctcccgaata
    21361 acctagtgca gttgcataac ctgcgaacct tatccgtcag ttcagcactc aaaacgtcgt
    21421 taaatgagcc tcttgcttat ctgtcaacca acgtgttgcg taagtccaac ggtgagattg
    21481 caaaagacgt ccagagtgcg acgctaacgg tacgtaaaga cgctgccttc ggtattaagt
    21541 tcaacggcgt gcaggccgcg cttggtgaat ctgtagaggt gaaaattgat atggggctcg
    21601 gtgataacct tttagtacct atataccctg ctgaaaacgg caaagtgggt acatccgagt
    21661 tcatgatcca gatcgatgag ctgaagtaat gagaatgggc cgtcggtagt actggcggct
    21721 taacgaaagt aactttttct gtaagggttg aagtgtgctt ctgatgtttt tagaacggat
    21781 cggaatttac tcacattttg atcgtgatga ttcaatacgt gacagagcat agttatcaac
    21841 aggatagaat aataataaag atcaatagag gatcaatagc agatcaaaaa ggaataacga
    21901 tcctcgcagc ccagtgatat cgggcctctc agcggatcag catccactgt acagggggag
    21961 aaatacacta tgtcgggtaa tccatccact gtaccgggcg cataatccac tataccgggt
    22021 agattcgata cactatagcg ggtaggatgt agatcttatg cacaaaacgg atctcccaat
    22081 atgtcaagta ataacaacaa gttatctctc gtcattgaag agtccctaaa agattcaggg
    22141 gaagttatac aactctttcc cacattaaaa cagaccattc aaccgaaagt tttactccgc
    22201 ctaggtgtgt ttgtgccgac tttgaaatct acggacaagg gggggaggaa gagccatcat
    22261 atcgatgcca ctgagccgct atcaaggtta tctattgtag agggtgaagg ttacagccag
    22321 atccaaatct acgggccgcg tctcgatatg gataccgatt tcaaatcatg gtgtgggatc
    22381 gtttatgcgt tgtctaaccg gccgcttgat tcagaaggtc agctggagct gaattttccg
    22441 gagtttgcaa aattttgtgg gtttgatacc aagcgcattg ataaacaact gaaagagcgc
    22501 tttgatgctt cgctgacgcg tatagcccgg accaacattt catttattaa gaaaataccc
    22561 ggtacagatg atcagataac gataaacatg cacctagtgg aatcgacgta ttacgacagt
    22621 tccacgggaa aaatcatcat caagccaaat tcgaagctca atacgctgta tcgcgtcgac
    22681 ggtaaaaccc gactctacct gaagacattg caaaaactgg cccgtaagga gtccgcacag
    22741 gccctgtacc tgtatctggc tgagctgccg agcacctttt accgcatcgg attcgatcgg
    22801 cttcgtggac gactccaact gtcatcacac ctcggtaacc agaatgccac ggttaaaaag
    22861 gcgctgggcc agcttaaaga gatcggtttt ctggaatact ccatagacaa agaaaacggc
    22921 gattacgtac tgaatattct aaagagaaac atgaagccat gaccgatgcc ggataatgca
    22981 cctgcctgcc gcgtgggtgg ctgcgttacc cggtatagtg gacattcttc tcctttccac
    23041 tctgctttac ccggtacaat ggatctccac tacaccgggt agtgtcaggc ctaaaattac
    23101 gatgcagccc ctcctcttca gccttacact gaatatttag tgtgtttttt caccaaaaat
    23161 gtgatctata cccggtacag tggagtcaca gaaatgtgat ccgtacccgg tatagtggat
    23221 gagaactact gctaatcggt taccgggaat gtggtggtac tgggtggaaa ggtttacccg
    23281 gtatagtgga tcgtggcagt gtcatctttc cggatagcat gacgtcacgg ttaggaaacc
    23341 gtacccggta tagtggatag cgaatggtgt acaaccctgt taacgtgatg atgctgaagt
    23401 gaaagtttaa cccggtttag tggagcgact taaagcgcgt accagtagct gatgggggag
    23461 cagcacccgg tacagtggag gggcggatat cgtcgtaaag gtcttaacta aaagatgacc
    23521 attcagtcgc agcattaaag aaatatatca tcgagtctca ggggttttaa gccggtagac
    23581 acccttccac caggagtcga aagacaagct atcatcgcag cgcataaata tgccgttatg
    23641 cttggtgaca acgctaagat agcctttatt tttcccgcag atcttctcaa aagactctgt
    23701 tgctttaata aaattttcac catccgtgat ggccggagaa tccagttcct tcgccagcca
    23761 tgccggatag ctgtccgcat taacccatac aaggcgttca acaactgaaa agaagacgcc
    23821 gacaaggcca atcgtccaaa gcgtaataac cagtaaacgc aaaattcgta aggctataaa
    23881 ttttgagtgc tgtagaggcg aaacgataaa aggcagtgag atcatgactg tgagtgagac
    23941 tgatgccgcc agcaaggtgt ataacaatga ccatgattta acataggcgg aaaaagcgat
    24001 cattaaacaa atcagaatga tgagcggtgc attgcttttc atcatgcccg tgctaatttt
    24061 cattcttttg gggctccatt ttgtacgagt tttgagagac tgcgtttgta atcatccgct
    24121 gccttagaaa agttaccgtc attctgtacc ggcgtaaagc gaatgccaaa aaacgcctca
    24181 atgacggagg aaagcgtcac tatcggtcct gacgaggcat gggcttttcg ctcccgagcc
    24241 tcacgctccg caaagtggat caccggtccc ctagaaaatg agagtgagtc atgggaagac
    24301 cagtctgttt atagccacag tagttaaacg caccccgcca gtccgtcgct gtttgacatc
    24361 atggccgcga attaggttaa cgtcgcccgg tatagtgtaa tcccaggctg aacctgtgtt
    24421 tgcagctggt ggccctgcat ccatcaccat gacaggcgct ggccgcagga atcgcgtgcc
    24481 ggatgaaggg tatccactat accgggtgaa tgatatgttg atccacgctg acgggtagtt
    24541 ttatgagcaa acccgcagcg agtggcaggc ctgtgtcatt cgcagtttta ctgatgggac
    24601 ggaaggttat ccacaaaaac ggtgaataac cctgtgaccg gaatctggat aatcatcatt
    24661 gcggcgacaa caacagaaag aagtggcccg gacttggcca ccctaccctg cttttacttc
    24721 agttcctcta tattcagcat aaattcagaa gttccaacga cgccggaagt cgaaggaaaa
    24781 atagggaagc tgcggctgtc acccagaccc agatccaact gaacattttt gctctcccca
    24841 ggagctgcgg aaaccccatt caccgtgacg ccgaaggctg catccttacg caccgtcagt
    24901 atcgcgtcct gtttcccggt tgcgatagac ccgtccttct tacgcagctg gctggcataa
    24961 agaaccgcca gcggtgtgtc atcggacgtt ttcagcgcgg tactaacgga gatggtctta
    25021 aggttttcca gacgaaccag gttgttcggc aagtaactaa attcgacggc gctttcttta
    25081 acgttgttca tctcgtcttt ggcctgcacg gtaagcgtgt aagtttcgcc gtcgttaagt
    25141 gaaggaaaga tcttcgggta attcggggca taaagattat ttccgaggtt tacccacgac
    25201 aactcaacag catcagatac cgggccaccg cgaagcgtca ttcgcgttaa agagggcttg
    25261 gtcagggtat ctgcgagctg aatacgaatg ttctccagtc catacaccgt tacactgctg
    25321 gtgataggtt tggtttcgta gctgatactg atggacggcg gcgtgttatc gaccgtcaca
    25381 gtcttatagt ccagactacc cgtattgttg aacgaatcct gggcgttgag ctgcacgtta
    25441 tagctgccct ccgggattag tttcaggttg aactcgtatg tataattccc ggttgctgtg
    25501 ttacgccccg ttagtgttcc ggtcacattg atatccgcgt tattgcggga tttgtcggac
    25561 agccacaccc ggctcagttt aacggtatcg aaatacgatc cgtctcccgg ctggttgatg
    25621 taaacctgaa ggatgttggt cgtatcattg tagtcatagc cagtgaccga aggccccagc
    25681 gtatgccaga cgacgttttc ccagataggc atgaagaagg tggattctgt tgtggaacgt
    25741 acctcataac cactatgcag atagccggtg gtgccgtttg taatcgcctg tgtcagggcg
    25801 acggtacacg acgttgaccc ggcagggatg ttgcaggtgg caccgccagt aattttttgg
    25861 tcgtagggcc ttgcctgcac attgaaacgg atctgggtca ggttcaccgg catatctgaa
    25921 gccttgaagt atttccaatt gacgctgtta aaccaggtac catcggacat ctgtcgctca
    25981 atcgccggac tcccccatgc aggacttttt accgaagccg ggtcccagtt cagccaggag
    26041 gcgtattgag tcagatcgcc tccggaccat tggtacgtgt tcactgggcg atagtagttt
    26101 atatccagag cgccctgggg aagcttcacc tcgacgtaca cataggtcgt gtcttctgaa
    26161 ataaccttag caccgccata attgttaaga atggtgatgc cgccgttacg gtatggcttc
    26221 cagttggttc gggggatacg gatgaccata cggtatgggt tttcgagtac agatagcccc
    26281 cgaacaaacg ggacgtaccc tgaagagatc cccgggacca cactcgtact gacgcgtgag
    26341 tcatgaaccg caaagggggt gtattcacct atctggtcat caaataaaaa gcgctgcggc
    26401 ggaatattca gcgtgtttcc ggctttatct gtgatgataa aattgaaggt aaattcttca
    26461 ttgagatcgc tggtgggcat accgggttga gtgaccgagt ccttgatcca ggggtaaaag
    26521 gcacgttttg ccgccgtatc gtaactgagt gtgttgtcgg acacgactgt gccatttccg
    26581 cgcttgatga ccagacggat cttgtctatg ccattcgcat catcaactcc atcagcaaag
    26641 atggaaaact ggccagatcc tccgcgccca agcccccaaa ctgggccact caggaccatt
    26701 tcatagcctg cgttctggct cgggtagatg ctggtatact ttggcgctgt agtgtcgatt
    26761 gtgaaatgat acgttgaaga gctgacgaca gcctgctgga gatccagagt atcgttgatg
    26821 acggtgaagg ccccttcacc gagtgcgggt aacgccaagt cctttccgta atattccgtg
    26881 ccgtctgcgg caacaatgcg atctgcgacg cttatctttc ctgtcactgc ggaatacatg
    26941 acctttttgt cactgtccct ggtcacactc actcgttcgt atcggtcaag gccggagatc
    27001 agattcaggg tcattgtgct tgccggatta aggtagctct gcgtggcagg cttaagcgtc
    27061 tttgccagct gattggtgtc agtatacgaa tagctgaaaa tctgagccgc cgccggataa
    27121 actgaagcta tggccgtggc aatgatacag cctgtcagaa ttttacgcat gtgatactcc
    27181 cgggatgtat ttgcccctat tttacgaaat cttttcacct gggcttgctg gttagccggg
    27241 gtatttcacg gcataaaact tttaaatgtt gccgtgatag caatgttgtc gggtcagaac
    27301 aaaacgaaag ttaaggcaac ttcaacaggg ctgattacgg tggaagaaaa gtaacttttc
    27361 tcttgagaag aaataggaaa agcagaaatt aggacgatta aatgaggtga aatgtcagca
    27421 acaggcgatg attgtccatc atcgtaaggg agggtacagg gtgccgctgc cttactggca
    27481 tcatgttgat gtccagctct tcatacccgt ttgtacctta gccggaactg ttgttcagta
    27541 caggtaaaag ttgtaataac tccccgctca ctgccgggga gtcattttaa atcagcacgg
    27601 cgccatcaga atagattagc tttcaaacca gggcgaatcg gcagagttcg cagattcttc
    27661 actttgctgt gactctccca gaaattgcac ttcacgtact ggtttacttt tccccaggct
    27721 ccgctggaaa tcatccatca caaactcaat atcatcacgg tcctcaggca agacaggcga
    27781 acttttggac atagtctttc cttcagttaa aagcgttctg catacagaac gcggacggag
    27841 acggcagtgt tgtcaaactc attatcgaag gtatccatcc actcggtcct gaacccctcg
    27901 cccagcaggt tatcgatccc ctgaagggac agaggcagga cggcagctaa acgcccgccg
    27961 ggaaccagat gagacgcagc ggccagcaga tgcagcttag cccggttgtc agcaaaagga
    28021 gggttcatca ccacgcaatc aaatttctga atctgattcg ccgccgacca ggccagaaaa
    28081 tcggcttccg tggtctcgta acctttagcc cgggagacca gacaattcag tgtatccagt
    28141 tcgatgccgg ttacgttgac acttttcggg aaacattgaa gcaaatcccc gtgcccaata
    28201 ttgggctcca gcagccgttc cccctccccg gccgcaataa tggatgcgac gtattcactg
    28261 agtcgcgccg acgacggata aaactggtgg gatttaatat caggcatggc gcccatatgt
    28321 ccgatgaagc gcatcacttc ggtcgggtca taatcaaacg tcacatggta gcttgtcact
    28381 cgggcaccga gaaatcgcag aacatccgcg ttcacctgcc gcgttttatc agcgttctca
    28441 ccggcatagc cgagcgatgt ccagctggac cattcctttt cattctgggt aaaatccagc
    28501 tctgcaagct gcatccgtga atgaaaatcg atgcagcgtt tcagtgccgg gaactcctgc
    28561 aacgtcgctt tggtatgggc cacgcgttca gcaggcagag ccagagggac gatggcagca
    28621 agaatgttgt taagccgttc ggcgatatca ggatgcacat caatgtgcat cgatccgttt
    28681 ttgtagaccc gaaaacgaat actgttgcca tccaggctga tccagtttct gaatccaaca
    28741 tgaccaacca tattctcaac catttttttc gtgtcataga ccggtttaac tgtttcgccc
    28801 cggaaataag aacagatcac ccgcaactcc gaaagtggca tgaggccgga ttctttaaac
    28861 tcaaccttca gttttaccca tttgtctttc acgccttcac atacgccgga ggtgatcatg
    28921 cgagtagaga agccaaatgc tttattggtt ttgtgttgac ggcttaatga ctgaaagacg
    28981 gcatagacac gatctttaat gaactgatgc cggtcattga gcaacgcgac aaccgtggta
    29041 acaacagcat cgagtgtaaa atcagggatc tcctgaggag gaaggtggta acggtcagca
    29101 gtaaactgct tatcccattc tttcttcttt tcgtcagaca tcacagacag tacatcggtc
    29161 agggccatga ccctcttcca gtattctgaa cgcagcgcgt tttctgcgta gctggcatcc
    29221 gcttctgaaa tgagatgatt gtatccggag aggtatgaat agagcttctg attcccgcat
    29281 ttcaggaact gagcggacat ggacctcagt aagctaagct catcgttata tgattcgacc
    29341 tgcctgttaa tgacatcgag ctcttcccgc atttcgggca ggaagttatc gtcgttaata
    29401 aggaggccag tatgaggcat ataagcaacc agagaactcc caggggcacc cattggcagg
    29461 gaatcagatt caacagcaga catagcacca gtcctttttg aagggtttat ctgataccag
    29521 gataacaacc ttgaacaaaa agctaatagg ttggctgaca tgaaaatgga gaaattaaca
    29581 ctgcggaaag tcagaaagac agatgtggtt ttaatcagaa gaaacgggcc ggagacagga
    29641 caaaaaaacc cctacatagt aggggcaacg gggggagagc ctgggtggct cgctctaaaa
    29701 tatcgtactt cttattgtag tcttctcaca ctctgaaata attcatttaa gcctgtgctg
    29761 ggggtgtttc ggattgcggt tctccgccat ttttatcgtt tttcttggtt ttgtaagtca
    29821 ctttttcccc tttcaatcgg gcttgccgat tttcggcccg gcgaagctgc ttgttttctg
    29881 attccgtaat ctcactttgc aggttcttga gcatctctag catgctccgg ttcagcgtaa
    29941 aggtaatttc ttcatcttcg ctggagtcgg tcacctccgg caactcgata tcaccgagtt
    30001 tctgcaatag actcgtcgta atgagtttga ccccttcgac ggctagcgag aactggtgga
    30061 acacgtgcgg gtttaaaatc ggaagggaca acacggatct gcccgcgtgg cgttttctta
    30121 atctggatac cgcttgaatc agccttttca aggtctgcga tcatcttttc gatccgcgca
    30181 acagcttccg tttcaccgaa ctcatcgata agggacagga ctgctacata ggaaatggca
    30241 tcggcatgga tcagtttttt gagatagaca cagcagccgg ttaaacgaat aagagcagca
    30301 attgtggtat tactttttcc ctcacgttcg gcaatacggt caagcgacca tccacccagc
    30361 gcctgaagac gtcggtagga ttcacccaaa gccacaggag aaagagccag gccacggttt
    30421 ccgtcaatcg ttgtcaattc gcagctgatc tcatcggctt tcatctcgat gcaaagaatg
    30481 gtgaggttag aatgcccttc tgccacagcc atcaaagcag cctttaaacg gcaatgtccc
    30541 tggcgaacgt acggaatgcc gtctttgatc agaacgcgga tagggtctac gtaatcaccc
    30601 cggatgtacg cattcttgat gttattgaga tgatcggcga cttctggcaa ctggtaataa
    30661 gcttccccca ttcccatctc acgggagttg aatccttccg tgatttgaac cacggttggg
    30721 ctgatagccc atgctttctg ttcagtgact tcattacaaa ggtcagggtt cgcgtcgcgg
    30781 aactgcttca taccactgac gaatcggccg gaaccggtca ctgttgaaaa agacacgagt
    30841 ggggatttaa agacccgtga gttagtttgc tgttgggaat cagtcatcag atcactcata
    30901 gatactccgt tgaattcttt tttgtcatac ctttcatctg tgcgtgaata ctaccggaaa
    30961 aaaaaatctc actttttcgt taaaaaggta ctttttgggt ctattccgaa ttgaacgagg
    31021 aaaattgttc tggcgatcag aacaaaaaga aaaaataact caaataaaac agatggttat
    31081 ggttaattcg taaaggataa acttttttct gagaagtgaa aaaaaattaa aaaatgtgtc
    31141 gaaatgacaa tctcgttaac aaaagccacg ttttttcctg ctcatacctt attggtagca
    31201 atccgccttt cggtaccata agtgtggcat accgataaag ctgaacggag aagactcgtt
    31261 gcgaaaacat acaccaatca tcaaatgggc ggggggtaaa accaaactga tgccgttcct
    31321 cagtaagcac ttcccacatg ataagagccg acgctgggtt gaaccgttta tcggcggtgg
    31381 cgccgtgttt ctgaacatgt ttgccaccga ggctctccta gcagacagca acccggacct
    31441 aatcaatctg taccgcaaca tacagcgaaa taaaccggca ttcatcagag aagtgcagct
    31501 gctggcggaa aggcattttg aagaagagga ttactacgtg ctgcgtaata cctttaacag
    31561 caccagtttt gatgatgccc cgctacaacg agcagccatt ttctacgcga tgaatcgact
    31621 cggatataac ggtctgtgtc gttacaacct gaagcgaaag ttctcagttc catggggaaa
    31681 gcgataccag ttcagtctgg atatccagaa ggttgattac ctctcattca ggctttcgag
    31741 cgttgaattg aaaaccgcag attttggtca gacgctggag tttgcaggtg gtggtgacca
    31801 aatatattgc gatccgccat atgacaaaat cagtaaaacc agctttgtaa gctatgatgg
    31861 tataccgttc gataagagcg cacatgttaa actggcggat atgctggtgg atgctaatcg
    31921 taaaggggcc tcagtggcca tatccaacag catgactccc tttacgctgg agctctatga
    31981 agaacgcggt tttgatattc atactcacaa cgcttatcgg tctgtcggca gtcaatcaaa
    32041 gtcgcgtaag aaggaaatag aaattctggc cgtccttcgt tgagcggcga tcagtagtta
    32101 gtcgatcggg cttatccatt cctcgaagct cagcacttcg gaatggtcac ggctggcata
    32161 gggaataaac tgaagctctt caattgatcg ctcccctctc ttaccttcgc atataaacga
    32221 tatgaacccc tgatctgcaa gttgggtaag gacattcatg attcgtacgt tcactgtatg
    32281 caactgaaga tcattcacga tatcggtaat tttgtgtgaa tggcgccggg actttcgcaa
    32341 ataatcaaag atgacaaact gggcattccg tcgcttcaat aatttttccg tcataaattc
    32401 ctgaattaat ccccccagca acatcaggaa cggacgaagt accccaggga acgttaacgt
    32461 ataagactgc gatgttttga ccagagtgaa ggacgtcagg aagctgaagt ttagtcgctt
    32521 ctttgaatac gggacataaa cagccaatgt cgtagacaag taagtaagca attcttccag
    32581 atgagcgcgt gtgatcatga ccgtcttatc gctattacga agaccgtgat aatcaataaa
    32641 tgccactctg gcacgactca gccactcgtg aatgcttatg gatacagtga acggtgcgtt
    32701 ttcatcggca gaaatgacgc cgtgctcagc gagttggtta tggataagca tagacagctg
    32761 gtaaaatgga aaacggcagg atggatgaca ctgtaggcca gttagcgtta gctttgcatt
    32821 atccaagtca tcgagcggaa aagtaacttt tcgggaaata atttttgctg acgttcttgc
    32881 tgacttgcgg atgaacccaa gtccaagatc attttgcata tatcactccg ttttaacaaa
    32941 atctccttaa cctatatgtt aagaagagac aaaaaattaa aaaatataat taataagatt
    33001 atcattttta atttaaaaga ttagttaata acgggtgttg aatttaaaat aaatcggctt
    33061 attttcggag tgtaaaaccg ggtaaaaacc cgatgcgtga gcaacagatt tgacgtgagt
    33121 gagtcggcac gacagtgcag aaagccggtg gctaatgtaa aaacggcctg tatttgggag
    33181 ttccggtcac tagacaccag gctatgctgt taatttaaac acagtcagta atgcgtaggg
    33241 cactgagtca aagtagtgga tctttctcac ttttcccgaa agatgatagc tatgccccaa
    33301 taaaacgtga tcctgaacat taccagtcaa cgcgaatcca tcattctgct ttttgcctgt
    33361 aaaagtaaaa aaatagctat gagaaccttt tgaaaacgca gctcgcactt ctgtaaaaat
    33421 aatatcaaaa ctaactacat caccaatgtt gaaagaatgt tcaattggaa aaccggcgtt
    33481 ttctgaaaaa gcttcagaaa caggctggta tgtcgagtag aaagactcca actgacggac
    33541 tgttaaaaag agattgttgt ttaactgctg tgcaatgctt gctgcaaact tagaattgtc
    33601 agcttgcagc aatgctaact gctcgctgtg cctacgtttc caggcttcaa gatcgggatt
    33661 gcagcgaagt tccgggatag tttcaaggaa ttcaatgcac tctttcagta ccagcttttc
    33721 ctctgtggct ggagacgttt tggagccatt gcatgtcaca cagacggcgt tcgcaatctt
    33781 tccagtccct ccgcattcaa agcactcacg ggtgataacg gcgtaatagt gtccatcttt
    33841 accgtagata ggctttacgg atgatggcag taaataacca tcctgagtaa aaaaaagaga
    33901 atgtttcatt ttttaaggtt ctccagtcga gcctccacga gctgtaaagc gtcagttacc
    33961 ttctgtacgt attgatcgtg cccttcttca aaatgatcaa aacgctttcc agccttgatc
    34021 tcttcaatgt tgcgatcgag tgtgcctttt gtaataagta gatcgttata agtattaatt
    34081 acagtaaact gtgcaatctt gtccataaac ggtccttagc attctactca cttgttaaca
    34141 ggtcacgaat ttcatgcagt tctgtaacct tagattttgg aatgatgata gcagtatttt
    34201 catcattcag aaggcttaat aaaagctgga gttttgcttc atctagagtg atctttacac
    34261 tttccgttgt ctttgtgatg gaagtgctgc tgatgttcgt aaataaattg gaatgttgtc
    34321 cgtcgaaaat taattcggtg agtttattac tgtcgtttag tgctggccgt acagtatcaa
    34381 tcagcccgat aaattcagtt tttaagcgtt cagttttttt atgagaaagc cgtttaatac
    34441 ccaatgaact gcgagtgatg gattttgttt taacttcctt aggaataaca tgaacaggga
    34501 gtgtttccgg gttatctagt ttctcatcgt ttgatgatgt ttgaacgctt gatgtcttag
    34561 ataaaacagg ttctggttca gttgatggta ctaaaagaga acacgattcc aaatgtgcaa
    34621 ggacttttgc atggtcattg gaatacttca acatgatctc aatcgcgagt gtttttttaa
    34681 tcaggtttag actgattaaa cgttgtagtt ctaaaggcat atcaaggatt ttaagctggt
    34741 tacgaatagc agtgctagac cgttgatagc gcatagcaag ggattcaacg gtagagccaa
    34801 gcttcagcgc ctctgccagt gcataagcta aggctacggg attaagaccg ttattcctat
    34861 tcccatcaag gttctccagg tattcttcac ttttattctt atagttcata acaactactg
    34921 aaatgagctg aatatcagct ccttccgcaa gagcaagaga tagtgctctg taacggcaat
    34981 gcccctgcct tacaacgtaa ttggagtctt tcttaacaac cttaattaaa tcgactcgtc
    35041 tgccctcttt ataggcttgt ttaatagaaa caatggtcga ttttactggt tcctgttcat
    35101 aacaaagttc accaattacc gcttcacgtg gattgaatcc agattcgata aaaagtgctt
    35161 taggactgat ctcaataatc tgatagtcgc agtgattcat ataacctcct ttttattaca
    35221 tgatacatta attgactcag tttcaaataa cccaaaagca aaaaacaatt aattcggtgc
    35281 ttgactgtaa ttttagttat gaaggaatta ggccatttta atttgaaatt tttaaatctg
    35341 aatgatttga aattaatata tcttaaaagc ttcagattga ttgatacttc ttccggctgc
    35401 attccctact tggctagagt ccgaacctga actttttagg tcagagttgg ttaacagacg
    35461 gaatttatcc acgttttcca caagttagat caattcttta gatcaagaga gatcaagaga
    35521 gtacaaaaaa gatcacaggt tccgtaaatg actgatttta ttatggaata agcatgatca
    35581 ggcattcagg tgcatgtaaa aagcatttga ttgaataaga aaagcattta cttgaattga
    35641 aaaggcattt agtttaatgc gagaaaagca tttgcacgaa taatctattc acatctaaat
    35701 gtatcaaggg ataaaccatg gtgggtaaat ttaaatgagt gataacaatg aggtaacaca
    35761 tccttttgat gtgaccaaca cggagacagg gaagacttat caactgagtc caaactcttc
    35821 gaaatcggtc caacccattg ctttactgcg cttaagtgtt tttaccccgg tgggaactaa
    35881 agagaagcga tacagaaatt ttgaggttga cgcttctgat gagttgtcga gcatggagct
    35941 ggcccgttca gaaggatacg atgacattcg gatcactggt cttaagcttt cgatgtcaac
    36001 cgattttaag tgttggctcg gttgcataat ggcgttcagc aaatatggtt tcgcttccga
    36061 taaaatcaca ttgtcattca atgagtttgc aaaaatgtgt ggtatcagtt ctacaaacat
    36121 aaacaagcga actcgctcac gatttcagga ggcgctagca aacctagctt ccgttgtgat
    36181 ttctttccgt gattcaaaaa ctgaacgttt tactgtcacg caccttgtgc agaaagcagt
    36241 gattgatcct aagaaggaca ccgtagagtt ggtgggcgat ccctcaatgt gggagcttta
    36301 ccggtatgat cataaaacct tacttagctt gcaggtgctt tcggttctcg ctaaaaaaga
    36361 ggcagcacaa agcctgtaca tatattttga ggcgatgcct gccggtacgt tattcgtaag
    36421 catgaagcgt ttgcgtgagc gacttctgtt gacgactcct gtacgaactc aaaaccagat
    36481 catcaggaag gcgatgctgg agttaaaatc aataggatat cttgaatatc aagaggttaa
    36541 gaaaggtaga gatatccagt tccagatttt taaaagaagt ccaaaacttg ctctggcaaa
    36601 acacagttga tgctccgggt atagtacgca caaaagttac tttggatgta atgaggagta
    36661 tgggcattca tccaaatgct ttttaatgtt tctccggatt tgaacaccct tccctgtttc
    36721 caaacttcct atcatttctg atacccgggt tcttctggta ttcgttcgaa tgcttttcag
    36781 aaaaaatcat gcttgctagt tgagacaacg taacctattc attttgctag tttaataaga
    36841 ctcattcata catatgcctt taactacact accctccatc gtgttgtatt gggctttaaa
    36901 cgcaaatccc tatactcctt gtgccactcg gcgtatttac agttccttaa cgcctgcaaa
    36961 cgactgggat atgagcctgg tcgacggcat tttatgaata tgcaggccaa tgaaggacct
    37021 agagaaataa cataacattc tgataataaa tgaaaattac tcaaattgga tatcaaggag
    37081 atgaaaagca tttgtttcaa tggagagcaa aaaagtgcct tttgtataaa agcatttgct
    37141 ccaatggcgg cagaaaagtg ccttttgtat aaaagcattt gcttcaatgg cggcagaaaa
    37201 gtgccttttg aaaaaagcat ttgtatgaat gctgcggtga aagttgcttt tggtcgggaa
    37261 gcagggctcg caagggacag tgtaaggtgc ctttgttcga aaagcatttg cacgaatagg
    37321 agaatggaag ttgcctttgg tcggaaagca tttgaatgaa tggggaagtg taagttgcct
    37381 ttggttggaa agcatttgca tgaatgagtc cctgataggt gtctttgttt gaaaagcatt
    37441 tgcatgaatg gattcagaag aagtgacttt tacgataagc atttgaatga atgggcaccg
    37501 cagacgcgca ttccccaatc agcatttgaa tgaataagct ccgcagtttt gctttcatcg
    37561 aacagcattc gtatgaatgt gctccgcaga ggtggctttc attgaaaagc atttgtatga
    37621 atgggggata cataatcctt gtgggtgaaa agcactcgca tcaatggggg aaaagtgact
    37681 tttggaatgt catccttacc gtgtcctttg ttaaatatct ggctggatga atgccataaa
    37741 aatggtaaag gggcagacga tgcccctctt ccctatttgg catgttaatt aaacgcacct
    37801 tctttaatcc gattacatgc gggattcttt tctgacattc gatggctaaa aaaacaccaa
    37861 gcgcaattaa gtcaaaaaaa aggtggttaa cagaaaaatg aaagaaaaat aaaaagtatt
    37921 atgccaatgc gttttcgtga attcaaatca acacggagta attatggaac tgacattgaa
    37981 tactaacgtt gaaagaggaa aaactaacgc ttttgcattt ttgaagtcca aacatgcaaa
    38041 agtgcctttg tttattctct tggttttagc aaattgcagt tttgcgtacg cgggttccga
    38101 tgatggagcc ttcggcgata tctgggccta catgagcgaa gccttgactg gtgcgccggg
    38161 taaaatcatc gcgtgcggca tgttgttctc ggtggcttat ttcggcgttg taaaacctaa
    38221 ccttggtttg gcgctggtat cagcattaat gatgctggtt atggcaaacg gtgaaaaaat
    38281 tatcagctcg ttcctggatg ctggtattcc tctgtgattt gttagaacaa taacaatgag
    38341 ggggatattc cccctctgtg gagtttttat gacaagccag tacgagatac cgccacatac
    38401 atatcgcttt ccatacagaa taaatatgcc gttactaatt ctattctggg atgcaaagca
    38461 attgggtata acattcgtga ctatcgcgag cggtaatatt tttgatttct ttataacttc
    38521 ggtagttgtg gcggtggtgt tctggtttgc atataagaaa gcagcagagg aagggattag
    38581 aggaaaacta aaacacaaac tgtggtggta cggctttttt cctggaaaat cagtgtttag
    38641 tagtcgttac tttaccgatc catttatcag aaacctctat tcttgatgga gctaagaatg
    38701 aaacttctca gcaggttaaa aattccattc attaaaaatt ccagtaatgg cgattttgat
    38761 aacaaggatg agacttcgca aaaaggaggt gaagaagtaa aaggaagttt tctggattca
    38821 aaagcacgct ttagtaaaga aattgaaggt tctgaaattg gaattacata tagtgcattg
    38881 attaaacgcg acgaaaaact cctacgtgtg aatacagttg caatgatcat tattggggtt
    38941 ctggttgtaa aaaatcaatt tctcactgat cctgtgacta tagtgctacc gccaaatatg
    39001 acggaagaag tcaaagttgt tggaaataaa gcctcagaat catataaaac gcagtgggcc
    39061 ttattcttta gtactttgat tggaaacata aacccaacaa acatcggttt tgttacaaca
    39121 actatattgg atgcgctttc tccggattta caggcaaaga cccgggaatc actacagcag
    39181 caaaccaata taatgcaggc tcgtggtgta gagcagtcat ttaagccgat cgatatgtat
    39241 tacgatacca aaaatgacat ggtctatgtc tggggaacaa agtcaactcg tttgattaat
    39301 gttccggaca aaactgaatc atccaaatgg acgtttgaat gggtcctggg aatgaaaaat
    39361 ggacgaccgc gcatagccta tgtgaaccag tattcaggta caccaaatat taagaaaatt
    39421 acaatcaacg gcaaagaaca actggcaaca ctcgataatc cgccgccgtc aataggtaac
    39481 taattatgac ttcaaaaaag gtttttcact gcaccgcgat tacagcagtg attttgctgg
    39541 caacttcgaa tgtggtattt gctgaagact atcagttacc ggcgacagta aataatccgg
    39601 tagttatgcc tgtcggggcc gatgaatttc agaacggtgt gaaaaatgcg ataattaaag
    39661 acactggcac tacgactgag accgtacaag caacaaaacc gacaactgct ctgccaagtc
    39721 tttcacctgc aagcagtgct tctcccgcag tcaatgaaat aaccggggcg ttgtcaaata
    39781 accctactct agctggttac cagcaacagg ttaaatcagg agattttgat agttacggaa
    39841 gaccagcagg gaaagaacct caacaaaatg cacaaagtac cgggtcccct tcaaaggcag
    39901 atgaacttta tgttgaagca cgtaatcgct ataaagaagt acaacgagta aacgttccgc
    39961 ctggtggcaa tattgtactc ccggtttctc gggggctgca aaacagaatt tcgacttcat
    40021 ttaaaaatgc atctgtaagt acttcaacgc cagctgatga agccagtatt ttcgttaatg
    40081 gcggggatgt ttttatttca acaaatacag ataagcctat aggaattatg ctttcagaag
    40141 attcagttcc tgaatctact tataacctga cactggttcc actggatgta ccaggggcca
    40201 tgatttcagt aacaacttct ttgagcccga caatgcaggc aaaacgagaa acttctcttg
    40261 ataaacaaaa ttatgatgag atgctggcac gctcacaatc agaagagttg actccttcag
    40321 accctcggca ggatgaccat aaacagcgga tcattgattt gttgactccg gttgcactgg
    40381 gtgaggttcc ttctggtttc agtttacaag aagaccgctt gtcacgcata ccaagctcag
    40441 agcagtcccc atgtaatttc aatatgtacg ccaagttagg tcaaagactg gtgggatctc
    40501 gggaattgat agatgtcgtt cttgttaaga atgataagcc ttacggacag gtagtggctg
    40561 atcagcaatg tataactgaa ggcgtggttg caagcgctct attcgataaa gcattccttc
    40621 agcccgggga agagaccgaa ctttatattg tgcgggataa attatttaaa gagcgtcaga
    40681 ctcgtgttac tacgcgccca agcctgatta aaagataata tgagaaaagc tacttttctt
    40741 acagtgatct cgtttggttt agttgtcttt ttgggtacca gttattacta ccaaactaag
    40801 tatatcccga aaggcaccgt gaatatattt cctgatgtcc accgacaggg tgagatcgtt
    40861 gatgatgcct ttgagaaaag cacgattgtt ataagcgacc caacgcttgc ggataaattt
    40921 gttggaagtt caaccgagtc tgtcggtcgg catgaagtta atgtaataaa agaattgggc
    40981 acgcaaaata atggttcgaa atttgttcat gatgagtctg ataaatcagg tttgacggtt
    41041 ggagataaat ggcctagggc tggtgaacct tatatcgtgc ctcagatgac agaatacgag
    41101 cggaatttga aagttaagcg attccagcaa ccaaagaatg gagttaataa tggacattaa
    41161 aaaggcctgg gaaaataaaa ctgtcagaat ctcagtaatt ggtgctgcat tgatggtatt
    41221 gattgttgtc atcagtcaat caatctttac cacaccagtt aaaaaagaga agaaaaccca
    41281 gaaaaaagat atgcaaactg gattccttat tgatgattca caaatgaaca agctgagtaa
    41341 tgaggaaagc cagaagactt ataacgaaat ggtaaggcag aaccgtattg accagaacgc
    41401 tgccaaggct gaccgtgaca aagctgaaaa ggctcagcaa gaaaacaaag cacagatagc
    41461 cagtcttgct tcgcaagttc agcaactcac tacgcagttg actgaaatgc agacctcaag
    41521 aaatggtaat cgcaatctcg atgcaggcgg accacgcaac aacgtcaatg aacaagcccc
    41581 agcaaagcca taccagctta atccaaatgc agcggtgaac ggggttagtt ctggatacgc
    41641 acctatatct ccgacccgaa acagtccaat gcggacaata acacagagtt caattaagac
    41701 aaacggcact gatggtgtca ttcaggtgat gccgatatca gagaaccgga tcagagaggg
    41761 gcgcgaggtt gttgcgggaa gtgaaaaggc cccaacgcga actattcgcg gcgatgggac
    41821 tgcacctgtt gatagtaaag ctcgtcatgc ggcccgtaag gatgaaatgt ttcttccagc
    41881 gacgtcaatt attactgggg tgctaattac cgggctggaa gctccaacga gtttgtcctc
    41941 taaagcagaa ccgatgccgg ttaccatgcg cattaaaaaa gacatcatca tgcctaacaa
    42001 ttacacgatg gatttgcgtg attgtaattt attgggttct gctgtggggg atctggcatc
    42061 tcagcgtgcg tacattaggg caacctccat atcgtgtgtc aactcgaaag gaaaagcatt
    42121 cgacgtaata gtggaagctt atgcggttag tgaaaatgac gggaaaaatg gcattcgtgg
    42181 gaatctgata agcagaaatg gaaatgcgat tgcaggttct gcgtttgcag gagggctgtc
    42241 agcattggcg gggagtctga gtccatcaaa ggtgtcttcg cttaatatag atccaaactc
    42301 tacggcacaa taccaatcgc caaacatagg tgcattagga gcgctggccg gagccggtgc
    42361 agctcagggg gggctaaacc gtcttgtgga ctactatacg tcaatagctg agcagcagtg
    42421 gccaattgta gaaattagcc cagggcgccc aattactttt gtcgttcaaa aaggggcgac
    42481 aatacctaca aacctgacaa gccgctgaga ataattatgg aaaataacaa cgacaatgaa
    42541 tcaccaaaaa agagtgagtc tgctgatagc ttaaatatcg acgcggctaa accggttggg
    42601 ggtatacgta ctaccaaaaa agtaacttta gagatcgata tcgagaaaac tcttaagtat
    42661 ctgatggtcg caggcctggc agttcttatc gtgatctatg gatacaaggg tggacgtttt
    42721 gtatatgaca acttcaaagc aatgtcgcaa ccggcctacc agattgccgt gctggatatg
    42781 caaactttaa gaaaagtatt taataagcaa aatccggtgt tggattcgca gcaatccaaa
    42841 actaactttg aaaattattt taaggcatta atgaaggttt accgtgaacg tggctatttg
    42901 gttattgatg catccttggc agtaactatt ccagagaatg ttgaaatagt ctcttatatc
    42961 gaccttggtg gaagttttgg tgaggtacaa tctcttccag gcgaacctaa agaaagtgag
    43021 attcgataat tgaaaaagtt acttttgtct ctgctgattt ccattacctc agcgtgcccg
    43081 ccgcttactc tggctgttga aaatgtatta aacaatcgtg atgccgctgc gttaatggaa
    43141 aaggcaaaag aaggtggagt atcaacgaaa cctttagacg gtgtattggc gaaaatgaaa
    43201 agctataagc ctaaagatgc tatttacatc cctactggcg gtctttattt gttccaggat
    43261 gaacgttctc agttgatggc ggtgaccact gacggacggt atacaatcac gggggggagc
    43321 gtcatggaca ttcttcagag aaagtcagtg ttatcagtag aggatatacg aaagtcgttc
    43381 tttattaatt tagacgacgc ccctttccct cttgaaactg tggcatcgat acctttagga
    43441 aacccaaaac tcaaacgtca agcggcaata tttattacgc ttgattgtga cggttgtcag
    43501 gatttaataa agaaattcta tgatgaacgc gaaaagtatc gggtcgatat agttttaatc
    43561 ccctcccccg gcgagccaaa gcaggaattg cgtcaattat ggtgcagtaa agaaaaaggc
    43621 aaagtgaatg atcttgatat cttgcgttgg cttatgggta ataaggccga tatagaaaaa
    43681 cgactcctga cgaaagaaga ggcagaagca tgtccggctg aaccgttagt cgcgtcatta
    43741 atgctggctg gcatttataa attacaggga gtcccctcag ttgtaagaca ggatggactc
    43801 gctggtaatg gcataccgaa agattttgat tactggttaa aacaaagtgt cgaaccctta
    43861 ctcaaaaacc catttgatac taattaaggt aatgaaatga acattaaaaa agttattttc
    43921 agcgctctgg ttgttggttc atcttcatac ctggcgggtt gtactattgg cagcgcagaa
    43981 tctgaatgcc cgggaattga aaaaggagtt atttgtaaag gcccgcgtga agttatggaa
    44041 ctgacgaaca atcgtgatga tctctccggc cttggggagg aagaggctaa ttcgggaaag
    44101 gggaaatcgg ctgtcaatga cagccagtac cctgcccaac taacgccgcc tggcgcagtg
    44161 cagtacccca agtcgtctgt acttgttaat aagccagtga catacacaac gacggaagta
    44221 aaaccggttg gacagctccc ggtgatgtat gatcagacat taaaaatggg ggcgccaact
    44281 tcaacaattg gaccacggcc aatcagcggg aatcctgtta attctaatgt acggatgaca
    44341 acgagttaca actcaaatgg gtcatccgtt aacccctttt tgcatccgtc tgctgaagtc
    44401 gtgaaaaaaa caactcaggt tgttactccc gcgcctgctc cgcgctatgt agcaccaaat
    44461 tctgatatca atgcaagtaa agacctttat tccattaaca atggacagcc agtaaaccct
    44521 acgctcagct ctgggcaggt acagcaatac cgtacacaag gttacaaaca ggcagtcgtt
    44581 gcaccagagc ctcttgctgt gcttcagcaa ggccgcgtca tgagaatcac ctttgcacca
    44641 tatacggatg ataatgacgc gctaaatctg ccttgctttg tttatgtgaa tgtcaaaccg
    44701 caaacttgga ttgcgggcaa aaattcaaca tcaaatccgg cacgaattgt tcctttggaa
    44761 gtccaggatg cggctcgtga aaatatgcaa cagcaacagc gagcaacgaa agcagtttcg
    44821 tcaaacggta ttgtgagaca actctgaggt cacgaatgag atcagctaac gtctataata
    44881 aagaattaag ccgtcatcag ttaggtggat ttattcccgt gtatgatcag attccgggta
    44941 cacattattt tctacttgac ggaaaccgac tcggttttat gtttatctgt agtccgtcgc
    45001 ccggtgtctt tgataatcag caggatgtgt taactgaatt atttaaaatg gacttcccag
    45061 ctgacaccat ctgtcagacc tcattaactg cgctgccaga cttgatcttg cacttaagtg
    45121 cctggtctgc cgttcgtggt ggtcgaatgg aaggtcatga taagcttaag ggggatttac
    45181 tcactgcata tcaactggat tattatgacc gaagcctgaa tgaacctttg aagccggacc
    45241 acgataaatt gatgcttcga gattttcagg tctggatttc cttttcaatt ccattgaagt
    45301 cagcacttcc ttctgaaata gaaaagactc gcattgatgc gctttactct gacttaatca
    45361 gtaagttgaa tacagtaggc cttttcccac acaaagttgg tgctgaaaat tggctttatt
    45421 gcatggataa gttattgcat ccgggaaaaa catcgcgttg gtctgaaggt catgttgaag
    45481 ctagcaccat gagacgccta aacgagcaga tcaatgtacc gggtcgaaaa tatacagtga
    45541 cagagaatca tttctcatct acaacgcaga gcaatgatat ttctgagcat cgttatttca
    45601 aacaattatc agtagtgaag tttccagaat ttgtaaactt tggctgcatg tatgaacttg
    45661 tagttaattg gttgaatgga cgtaaaacga tctttagccc tttcatgata acccagactg
    45721 tccatttcgc cgatccttta aaattatcaa gagaaaacgt cagatataag gcaattacta
    45781 acaaacaagc cagtattcca acagttttga cgttttgtcc aagattaaag gatatggata
    45841 atgattacat gacgataaca cgcgaactgg aagatggggc tcgcctccta cacagttatc
    45901 tgacatttac tgtaatgggg aattcagcag ttgatgttca gtctgccgca gaccagttaa
    45961 aatcatttta tctggaaagt cgtgttaatg ttgctgatga ctcgtacata gtatttccct
    46021 catttgtttc aagcctgccg atgtgtaatg acccaaagac aatactagaa ctggatcgtt
    46081 ttgaggtcgt gagtaatact ggtgctgcgc acatgacccc catatttggt ccttggaaag
    46141 gtaatacgga cagacccgta cttaaccttg tctcacgaga aggacagctg ttggggctgg
    46201 atatcttcaa gacttctgcc agctataaca tggtcgttgg tgcaacgtct ggtgctggta
    46261 aatcattctg ggtcgcatac atcattaata attatctagg cgcaggtccg cgttctaata
    46321 atctgatcca ttatcgagat acttttgagg gattcaaaaa caattcttat gatgcatttg
    46381 accctgatgg agcccagatt ttcgtagttg atgtcggacg ctcataccaa ggtatttcgg
    46441 aacaatatac taatagtcag tttatagact ttgggaaaaa gcctgatttt acgctaaatc
    46501 cattcgcatt tttgactgat gtgactgttg gagagcgtgt ttttgatgaa gcacctgtct
    46561 ttaatgacga ttcaaataat catgatgatg ataaggataa agtagcccag accattatgg
    46621 ttctaaacca gttaaaaata atggcatctg aaaaaggaaa tattgacgat tttcaacaat
    46681 cggtcatgct gcaactgatt agtgaagaat ataacgaatc acgtaaagtt ggacgtacag
    46741 gctctattac tggatttgcg cgacgctgtt ctaaccatga agataagcgt attaaagata
    46801 tcggtgacca gttggggcaa tggtgtgaag gagggattta tgggaatcgc tttacagaaa
    46861 atctcccgcc aataaatttt gatagccggt ttattgttct ggaacttgaa gaattgaagg
    46921 ggaccccaca ccttcagact gtcgttctga tgtcgattat tcaggccgct cagcatgcaa
    46981 tgtttatcaa gaaagacgga cgccgccggt tgttcatact tgatgaggca tgggagtata
    47041 tccgtccgga caatgcttca ggttccagta atcattcaaa tcaatttttc tcctctttcc
    47101 ttgaggccgc ttggcgccga ttcaggaaaa cgaactgtgc cggtatttgt attactcaat
    47161 cctttgaaga ttacttcacc tcatcggttg gtcgcgcact aacggctaac tcgccgtgga
    47221 agatcatcat gaagcaggaa aaggaaagta ttgaagccat gaaggtgaac aactacttct
    47281 ccacaactga tgcggaatat gagcgtatga aaaatattcg aacggtaaag cgtgcatttt
    47341 ccgagatgct cgtacgattc gaaaatttcc aggagatatg tcgcctctat gtagatagaa
    47401 aaatggagct ttgctttacg actgacagca ccgatcgtgg aaaactatgg gaaatccaaa
    47461 gccgacttga ttgctcttac ggcgaagcaa ttgagattct gtacgaacag gaagttgcga
    47521 gcaaatcagc tgcctgacgt tttaagttac tggttacctc attttgtttt gactgaggta
    47581 tccagtacgt ctttgaagta aatagttgtt agctgtcatt gcctgccctc ccccgaaaca
    47641 atagcccggg ttaagaatat ctagctcgga ttaagtcctc tcaaaccgtc atacatgaaa
    47701 ttcgatgcct ttgtcattct tcttttgaag gtgaatgaat atgatgaaat ttactccaga
    47761 attattctcc ctaccaggct tttgctgtga agttaatcag atcaggtatt atgttttggg
    47821 tacaagaacc aaccaagggt gggtgtggtt tttgtatggc agatacaaac atcattttta
    47881 cggttataat aaataaccat tatatagatg aatttatcgt tttttctttt aagtctttga
    47941 agtgattggg tttaatatct agatgatggt tgtataagct atacgttaat tagatttgag
    48001 aatgcaactg ttatgcgatg cgtagatatt gctaaggtga atggtttgtt aaaacaacca
    48061 aaaaataagt aagatcaggt cttatctaca taataactta accatctaac attctggttg
    48121 gaataagaaa ccattttaag cttattcaat aacgaaattt aaccacttga atcgactgac
    48181 catggaggga agctggttgc gtaccgtggc atttaacccc cttaatcgcc agaccatggg
    48241 gggaagctgg tcgggtactg tggcatttaa cccccttaat cgccagacca tggaggaaag
    48301 ctggttgcgt accgtggcat ttaaccccct taatcgccag accatggggg gaagctggtc
    48361 gggtactgtg gcatttaacc ccttaatcgg cagaccatgg agggattatt gccgggtacc
    48421 aggccattta accacttact cgtctgtaca cgatgggatg ctggtcgcgt agcgcggaat
    48481 tgctccccta ttcaccagtt ctttgctaaa ggaaagcaag tctaaaaggt tttccggcca
    48541 aaactttttt tgtttcacta cagttattcg cccttccgtt cagcgtgata aaatatacaa
    48601 aaacgtagag gtaacccata aaagtaaaag agcttataaa ccagcttaaa aggctcgacc
    48661 cggatgcgta tgtagtagtt gctggatttg aaaactagtg tactggacac gtggctgaag
    48721 cagataccat taaagaatgc atgacaattc ctgtacgagc ggacagcatg tcaggtgacc
    48781 gtacgcttgc aaaagatgga tcaccttcag tctagttgag gtggggagat gactaccgca
    48841 cggaatactt cgttagtgca atagacgaca cggatgaatt ggcctaatta tacgaattgc
    48901 caaaaaactc tcttattgtc aattgggggt atgggcaatt catattgatt gccaaatatt
    48961 ttttgggtat tcgggatgtc ctaacaacag gatttaccga ataccctatt tttttgccaa
    49021 atatttcgtc attctaagtc gagatccagg ccgaatatct tttaatctaa tccactcaat
    49081 agacagattc tggtgggata cttattgtgt accgtcgtat taaacccact taaccgctag
    49141 ggcgtcacgt tgtcctgtct gcaagccgag gcaatttccc ctttagccaa tgaccacggt
    49201 gcctgctggt cgggtaccgt gggatttaac ccccttaatc gccagaccat ggaggcaagc
    49261 tggtcgggta ccgtggcatt taaccccctt aatcgccagg ccatggggag gctggtcgcg
    49321 tagcgtggca tttaaccctt taatcgccag gacatggggt catgctggtc gggtaccgtg
    49381 gtatttaact cccttattcg ccagaccatg gggaatgctg gtcgcgtatc
//
LOCUS       DAOFQD010000021        47624 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.021, whole genome shotgun sequence.
ACCESSION   DAOFQD010000021 DAOFQD010000000
VERSION     DAOFQD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.021"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            43..675
                     /gene="comR"
                     /locus_tag="QBF46_001270"
     CDS             43..675
                     /gene="comR"
                     /locus_tag="QBF46_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDQ6673473.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(737..1276)
                     /locus_tag="QBF46_001271"
     CDS             complement(737..1276)
                     /locus_tag="QBF46_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDQ6673474.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1503..2807)
                     /gene="ndh"
                     /locus_tag="QBF46_001272"
     CDS             complement(1503..2807)
                     /gene="ndh"
                     /locus_tag="QBF46_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDQ6673475.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3207..3749)
                     /locus_tag="QBF46_001273"
     CDS             complement(3207..3749)
                     /locus_tag="QBF46_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ6673476.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPHENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3772..4797)
                     /gene="nagZ"
                     /locus_tag="QBF46_001274"
     CDS             complement(3772..4797)
                     /gene="nagZ"
                     /locus_tag="QBF46_001274"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDQ6673477.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEKGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4808..5632)
                     /gene="thiK"
                     /locus_tag="QBF46_001275"
     CDS             complement(4808..5632)
                     /gene="thiK"
                     /locus_tag="QBF46_001275"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDQ6673478.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHLQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5613..6254)
                     /gene="lpoB"
                     /locus_tag="QBF46_001276"
     CDS             complement(5613..6254)
                     /gene="lpoB"
                     /locus_tag="QBF46_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDQ6673479.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6268..6645)
                     /locus_tag="QBF46_001277"
     CDS             complement(6268..6645)
                     /locus_tag="QBF46_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDQ6673480.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6648..7007)
                     /gene="hinT"
                     /locus_tag="QBF46_001278"
     CDS             complement(6648..7007)
                     /gene="hinT"
                     /locus_tag="QBF46_001278"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDQ6673481.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7341..9530
                     /gene="fhuE"
                     /locus_tag="QBF46_001279"
     CDS             7341..9530
                     /gene="fhuE"
                     /locus_tag="QBF46_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDQ6673482.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9590..11023)
                     /gene="ptsG"
                     /locus_tag="QBF46_001280"
     CDS             complement(9590..11023)
                     /gene="ptsG"
                     /locus_tag="QBF46_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDQ6673483.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11318..12115)
                     /locus_tag="QBF46_001281"
     CDS             complement(11318..12115)
                     /locus_tag="QBF46_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ6673484.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVNFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12126..13130)
                     /gene="holB"
                     /locus_tag="QBF46_001282"
     CDS             complement(12126..13130)
                     /gene="holB"
                     /locus_tag="QBF46_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDQ6673485.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13127..13768)
                     /locus_tag="QBF46_001283"
     CDS             complement(13127..13768)
                     /locus_tag="QBF46_001283"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDQ6673486.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13758..14780)
                     /gene="yceG"
                     /locus_tag="QBF46_001284"
     CDS             complement(13758..14780)
                     /gene="yceG"
                     /locus_tag="QBF46_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDQ6673487.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14783..15592)
                     /gene="pabC"
                     /locus_tag="QBF46_001285"
     CDS             complement(14783..15592)
                     /gene="pabC"
                     /locus_tag="QBF46_001285"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDQ6673488.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLHDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLASIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15712..16953)
                     /gene="fabF"
                     /locus_tag="QBF46_001286"
     CDS             complement(15712..16953)
                     /gene="fabF"
                     /locus_tag="QBF46_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDQ6673489.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17041..17277)
                     /gene="acpP"
                     /locus_tag="QBF46_001287"
     CDS             complement(17041..17277)
                     /gene="acpP"
                     /locus_tag="QBF46_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDQ6673490.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17488..18222)
                     /gene="fabG"
                     /locus_tag="QBF46_001288"
     CDS             complement(17488..18222)
                     /gene="fabG"
                     /locus_tag="QBF46_001288"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDQ6673491.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18235..19164)
                     /gene="fabD"
                     /locus_tag="QBF46_001289"
     CDS             complement(18235..19164)
                     /gene="fabD"
                     /locus_tag="QBF46_001289"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDQ6673492.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19180..20133)
                     /gene="fabH"
                     /locus_tag="QBF46_001290"
     CDS             complement(19180..20133)
                     /gene="fabH"
                     /locus_tag="QBF46_001290"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDQ6673493.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20201..21271)
                     /gene="plsX"
                     /locus_tag="QBF46_001291"
     CDS             complement(20201..21271)
                     /gene="plsX"
                     /locus_tag="QBF46_001291"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDQ6673494.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21352..21525)
                     /gene="rpmF"
                     /locus_tag="QBF46_001292"
     CDS             complement(21352..21525)
                     /gene="rpmF"
                     /locus_tag="QBF46_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDQ6673495.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21577..22098)
                     /gene="yceD"
                     /locus_tag="QBF46_001293"
     CDS             complement(21577..22098)
                     /gene="yceD"
                     /locus_tag="QBF46_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDQ6673496.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22297..22881
                     /gene="yceF"
                     /locus_tag="QBF46_001294"
     CDS             22297..22881
                     /gene="yceF"
                     /locus_tag="QBF46_001294"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDQ6673497.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22993..23952)
                     /gene="rluC"
                     /locus_tag="QBF46_001295"
     CDS             complement(22993..23952)
                     /gene="rluC"
                     /locus_tag="QBF46_001295"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDQ6673498.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24525..27710
                     /gene="rne"
                     /locus_tag="QBF46_001296"
     CDS             24525..27710
                     /gene="rne"
                     /locus_tag="QBF46_001296"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDQ6673499.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27906..28859)
                     /gene="flgL"
                     /locus_tag="QBF46_001297"
     CDS             complement(27906..28859)
                     /gene="flgL"
                     /locus_tag="QBF46_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDQ6673500.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28871..30514)
                     /gene="flgK"
                     /locus_tag="QBF46_001298"
     CDS             complement(28871..30514)
                     /gene="flgK"
                     /locus_tag="QBF46_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDQ6673501.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGEGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASALLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30580..31521)
                     /gene="flgJ"
                     /locus_tag="QBF46_001299"
     CDS             complement(30580..31521)
                     /gene="flgJ"
                     /locus_tag="QBF46_001299"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDQ6673502.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31521..32618)
                     /gene="flgI"
                     /locus_tag="QBF46_001300"
     CDS             complement(31521..32618)
                     /gene="flgI"
                     /locus_tag="QBF46_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDQ6673503.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMHVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32630..33328)
                     /gene="flgH"
                     /locus_tag="QBF46_001301"
     CDS             complement(32630..33328)
                     /gene="flgH"
                     /locus_tag="QBF46_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDQ6673504.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33381..34163)
                     /gene="flgG"
                     /locus_tag="QBF46_001302"
     CDS             complement(33381..34163)
                     /gene="flgG"
                     /locus_tag="QBF46_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDQ6673505.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34335..35090)
                     /gene="flgF"
                     /locus_tag="QBF46_001303"
     CDS             complement(34335..35090)
                     /gene="flgF"
                     /locus_tag="QBF46_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDQ6673506.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35110..36315)
                     /gene="flgE"
                     /locus_tag="QBF46_001304"
     CDS             complement(35110..36315)
                     /gene="flgE"
                     /locus_tag="QBF46_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDQ6673507.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36340..37035)
                     /gene="flgD"
                     /locus_tag="QBF46_001305"
     CDS             complement(36340..37035)
                     /gene="flgD"
                     /locus_tag="QBF46_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDQ6673508.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGYGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVVISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37047..37451)
                     /gene="flgC"
                     /locus_tag="QBF46_001306"
     CDS             complement(37047..37451)
                     /gene="flgC"
                     /locus_tag="QBF46_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDQ6673509.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37455..37871)
                     /gene="flgB"
                     /locus_tag="QBF46_001307"
     CDS             complement(37455..37871)
                     /gene="flgB"
                     /locus_tag="QBF46_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDQ6673510.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPSYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38027..38686
                     /gene="flgA"
                     /locus_tag="QBF46_001308"
     CDS             38027..38686
                     /gene="flgA"
                     /locus_tag="QBF46_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDQ6673511.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38762..39055
                     /gene="flgM"
                     /locus_tag="QBF46_001309"
     CDS             38762..39055
                     /gene="flgM"
                     /locus_tag="QBF46_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDQ6673512.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39060..39476
                     /gene="flgN"
                     /locus_tag="QBF46_001310"
     CDS             39060..39476
                     /gene="flgN"
                     /locus_tag="QBF46_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDQ6673513.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39516..41051)
                     /gene="murJ"
                     /locus_tag="QBF46_001311"
     CDS             complement(39516..41051)
                     /gene="murJ"
                     /locus_tag="QBF46_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDQ6673514.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41161..42084)
                     /locus_tag="QBF46_001312"
     CDS             complement(41161..42084)
                     /locus_tag="QBF46_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDQ6673515.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIEITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42086..42733)
                     /locus_tag="QBF46_001313"
     CDS             complement(42086..42733)
                     /locus_tag="QBF46_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDQ6673516.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42744..43328)
                     /gene="rimJ"
                     /locus_tag="QBF46_001314"
     CDS             complement(42744..43328)
                     /gene="rimJ"
                     /locus_tag="QBF46_001314"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDQ6673517.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43564..44772
                     /gene="mdtH"
                     /locus_tag="QBF46_001315"
     CDS             43564..44772
                     /gene="mdtH"
                     /locus_tag="QBF46_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDQ6673518.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44836..45483
                     /gene="grxB"
                     /locus_tag="QBF46_001316"
     CDS             44836..45483
                     /gene="grxB"
                     /locus_tag="QBF46_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDQ6673519.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45617..46177
                     /locus_tag="QBF46_001317"
     CDS             45617..46177
                     /locus_tag="QBF46_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6673520.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46283..47329
                     /gene="pyrC"
                     /locus_tag="QBF46_001318"
     CDS             46283..47329
                     /gene="pyrC"
                     /locus_tag="QBF46_001318"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDQ6673521.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47403..>47624
                     /gene="dinI"
                     /locus_tag="QBF46_001319"
     CDS             47403..>47624
                     /gene="dinI"
                     /locus_tag="QBF46_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDQ6673522.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESA"
BASE COUNT        11497 a        12890 c        11862 g        11375 t
ORIGIN      
        1 tataataacc tttcattcac agtcaccagg tacaacggca tcatggcaac tgactcaaca
       61 caatgtgtaa aaaaaagccg tggccgccca aaagtgttcg acagggatgc cgcgcttgat
      121 aaggccatga aattgttctg gcaacacggt tatgaagcga cttctcttgc ggacctcgtc
      181 gaagcgaccg gagccaaagc gcccacgcta tacgcggaat ttaccaacaa agaggggtta
      241 tttcgtgccg ttctcgaccg ctatatcgat cgttttgccg ctaagcatga agcacagctg
      301 ttttgtgaag agaaaagcgt ggagtctgcg ctggctgact attttgctgc catcgccaac
      361 tgctttacca gcaaagacac cccggcaggc tgcttcatga tcaacaactg caccaccctc
      421 tccccagatt caggagatat cgccaatacg ttgaaatcac gccatgcgat gcaagagcgc
      481 actttgcagc agtttttatg tcaacgacaa gcgcgcgggg aaatcccgac ccactgtgac
      541 gtgacacatc tggcagaatt ccttaattgt attattcagg ggatgtcgat cagcgcacgc
      601 gaaggtgcat cgctggaaaa actaatgcag attgccggaa cgactttgcg tttatggccc
      661 gaactggtga aataagtcgc aaaaaaagcc cctcagcctg agcgaatgag gggccaaatt
      721 gcagagtaca gccttgttat actttgttat tcaaaatcag ttgcccatta ctatctagcg
      781 ggatctgcgt acccggatcg cggtccatgc ggattttgcc ctgctgatcg ccaatcttat
      841 aggtcacatc ataaccgagc attttttctg acttgtcata caccgtttta caacgctgtt
      901 gcgtagtcgt gtaagtatcg ctttcctgga gagagccctg gatctggtta ccggcatatc
      961 caccacccag cgcccccaca acagtggcga catctttacc gcgaccaccg ccaaactgat
     1021 gccctatcac gccgccagca acagcgccga gcaccgaccc ggtaatgcga ttttcatcct
     1081 gcaccggtcg acgatgggtc actgtgacgt tgcgacactc ctgacgcggt gttttaaccg
     1141 tttccttgat tggggttgca gaaacaacct gagcgtattg cgggccacgt tcaaacacgt
     1201 tcagactggc cactgccgct acgcccagcg cagctgcgac accaatcccg atacccgcca
     1261 acattgattt attcacggga acacctcctt ctttgcagtt atgccaatca tgcaagcgaa
     1321 ggagaaaagc agctatcaga cagaagatga aaagcgcggg aatggctgga aatacttaat
     1381 gattcggagg agtctgaagg aatgcctgat gcgacgctta acgcgtctta tcaggcctac
     1441 cgctcgtacc atttgtccag ggcggatgag gcgtttatgc cacatccgcc agtgtacgtc
     1501 gattaatgca acttcaaacg cggacggata acgcggttaa tactccccac cagcatcatt
     1561 aatccggttt taaagtaacc atgcagcgca atctgatgca ttcggtatag cgagatatat
     1621 acaaagcgcg caattcgtcc ttcaatcatc attgagccgc gcgtcaggtt acccatcagg
     1681 ctaccaacgg tggagaagtt cgacagcgat accagtgaac catgatcttt atactgataa
     1741 tttttcagcg gcttaccgtt tatctgcgcc agaatattgt tcattgcgca ggtcgccatc
     1801 tggtgtgcag cctgagcacg cggcggaaca aagccccctt ccggacgcgg gcatgacgcg
     1861 cagtcgccaa tagcgtaaat gtctggatcg cgggtggttt gcagcgtcgg ttccaccacc
     1921 agctggttga tacggttagt ttcaagacca ccgatatctt tcaggaagtc tggcgctttg
     1981 atcccggctg cccataccat cagatccgcc tcaatatatt cgccatcttt agtgtgcagg
     2041 ccgccttcat cagcactggt gaccatggtt tgcgtcagca cgcgaacgcc aagtttcgtt
     2101 agctcgttgt gggccgcagc agagatacgt ggcggtaacg caggcaaaat acgttctccc
     2161 gcttctacca gcgttacgtt cagggcttcg ttggtcagac ctttataacc gtagctgtgc
     2221 agttgcttga ccgcgttatg caactcagcg gagagttcta cacccgtcgc gccgccaccg
     2281 acaatcgcaa tgttcacttt gccattcgcg cccaggttgg cggagtattt caggaacaga
     2341 ttcagcatct cctggtggaa gcgacgcgcc tggtgcgggt tatcgaggaa aatgcagttc
     2401 tctttgacac ctggtgtatt gaaatcgtta gaggtgctac ccagcgccat taccagtgtg
     2461 tcataggcga ttttacgttc cggaaccagc agttcacctt tctcgtcgcg cagttctgca
     2521 atagtgattg ttttcgcttc acgatcaata tcaatgacgg aacccagctg gaactggaaa
     2581 ccatgattgc gcgcatgggc cagatagctc aacgcatcga cgccttcatc aagcgagcca
     2641 gtcgccactt cgtgcagcag cggtttccac aggtggctgt ggttacgatc gaccagcgta
     2701 attttggctt ttttcttgcg tcccagctta tgccccagct gtgttgccat ttccagccca
     2761 ccagcaccgc cgccgacaat cacaattttt ttcaatggcg tagtcaacgt gaccccctta
     2821 aatttattaa ccaattgtta attaatggtt attaacatag ctcttaatta acaacaggtt
     2881 acagcaatga aaatgttctt cgctattgag aataacatga atggtgcatt ggtcatacca
     2941 aaattgatgt taatcaagtt ttgttgctga aaagataggc gactttaccg ggtgagatca
     3001 aaaaacagag tttattgttt attatttgag cgaatttgtg agagtgatat agagtgaggg
     3061 gtgtatgcag ggcgttcggt aggcctgata agaggcgcat caggcattga tgctcgactg
     3121 tcggatgcgg cgtaaacgcc ttatccgact tacagaattc ggagcgcatc aggtaccggc
     3181 aaggttacct gatgcgacga gggcatttac ccgagggttt tgaacgcttt aatgcgctgc
     3241 aaatgcgggg agatattctt gaatttgtgc gtctgctctt cgtcccagac aatctcgtaa
     3301 taatgatgca actcttcaga tgtccgctgg ctgttaagcg cttcatcatt acgcgacaaa
     3361 atcaccaggc aacgatcgcg attcttctcg cggaagttgg tcacacactt agtggcaata
     3421 tcggcatact cttccgggcg atcaatcttg ccttccatgt tctcatgagg gaacaaatta
     3481 gggttgaaga tcacctggcg gatatcgcaa agaaaaccaa tccgttccgc ccagtatccg
     3541 cccagaccaa cgccgcaaat cagcggacgc tcgtcgacgt tcagttgcaa cattttgtcc
     3601 acttctttga gcagatgctg catatcatgt ttcggatgcc gcgtactgta gcttatcaag
     3661 cgtacatccg ggtcaataaa ctgcaattgt aagactttct cgtggttacc cggactgtta
     3721 gagtcaaaac cgtgtaaata gataatcatc gcttcctcac ataagccagg gttagtgacc
     3781 tgctttctct tcctgccagc gttcatgtaa ctgattcaga cgggtgctga tcgccttcca
     3841 gcgagccgag tccatcagtt cctgtcgcga aaatgaacct ttatgataca aacgtgtaac
     3901 acgttctgcc ttgatcggtg acagattatc taacacgctg acggcccctt tacgattatt
     3961 gcagaccagg atcatatcgc aacctgcgtc cagtgatgcc tgcccgcgtt cggcataact
     4021 gcccataatc gcggcacctt ccatcgataa atcgtcagag aaaatcacgc cgtcaaaaca
     4081 cagttcctga cgcaaaacgg ttttcagcca gtaggaagaa ccgctcgccg gacgcggatc
     4141 aacatcactg tagatcacat gcgcaggcat aatggcgtcg agtttatttt cgcggattaa
     4201 cgtgctgaaa accgacatat ctttggcacg aatttccgct tgcgggcgtg ggtcgcacgg
     4261 cgtctctttg tgtgaatctg cagttactgc accgtgtcct gggaagtgtt tcccggtcgt
     4321 tttcattccg gcttcatgca tcccatcaat aaaccgactg gcgattgcca gggctttttc
     4381 tgggtcggca tgataagaac gctcgccaat cgccgcgctg atatgtccta catccagcac
     4441 tggcgcaaag ctgatatcaa tatccatagc gatcatttcg ctggccatca gccaacccgc
     4501 ctcttgcgcc agtttgccgc ccttttccat tcccaacagc gcagcaaagg attgtgctgc
     4561 cggtaagcgg gtaaaacctt cgcgaaaacg ctggacgcgt ccaccttcct gatctaccgc
     4621 caccaccaga tgattgcgcg atgctgcgcg gatctggcgc accagttcac gtaactgggc
     4681 aggatcatga tagttacgcg taaagagaat cagccctccc accagcggat gcgccagtat
     4741 ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc aacattactg gacccacact
     4801 gctctcctta ttgttttatt aatagctgcc gccaggtgtc atcggccagc ctgataaatt
     4861 gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc tgctttgagc atcagcagcc
     4921 agggaaacca tcgcctgacc tgacgccata attgcgccgg ataaatcttc gcgcgagtgg
     4981 catagtcatt gaccaattgc aggtgctgtt cagtattttc cacccacacc gccgccagtt
     5041 ccagcgcgat atcaccatct cctgcatact cccagtcgat gagttttaac cctgacgcgc
     5101 tatgcactaa atttccggcg tggacatcca tatgcaatgg acttaagcgt aaaggccgtg
     5161 gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca acccactgtc cgccgcgccg
     5221 gatcgctttg ctgccagtac agttccagta acggcaacag cgttattcgc cagccaaaac
     5281 gtggttgttg atgtagataa tacagcaagc ctgccagttc gttggtatcc ggcaaatacg
     5341 tttttaccgc gccgggcaga tagtcgacta ccatccagtc acggagatat aaatgcggct
     5401 tcggtgcaat gcatgcgggt agttgtgata aagcccgata ctggcgcaag aacgcggact
     5461 gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg ctggccctgg tgttcaatga
     5521 gaaaactccc gccactaagc ccactattaa acgtcgtgac gggatgaaac tgcgggaaaa
     5581 agcgcgacag caattcgtcg cgcgtgatgg gattattgct gcgaaacggc acctttacct
     5641 gaccagataa tttcgcccgt ctgcaccagc atcagctgca tttgtagggt cggagcgtta
     5701 acgttgccag aggcgctgga gtatagcacg taatgagcgc cgacattgcg ggcaatgcct
     5761 atggctttac tacgggtacc taaactgtcc tgcggcgaca aacctaactg ttgcttagcc
     5821 atcgacagct gctgggcgga aaccagggta aatttaccgt tattagccag cgcatttcgc
     5881 agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag tacggttgtt aacgctatca
     5941 accagcagga cgctacccgc agtcaccccg tcagccccaa gcatcttact gaccatcggc
     6001 tgcattgcgc cattccagtc ataatggcga atatgcggcg caggcggtgc agtttgatct
     6061 tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca ccgagggcac tgtggggaca
     6121 ggctgttgtg gctcggctgg ttgttccggc gctggtttca cttcttctac cggtgcaggt
     6181 tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg cggtaatcaa ggcgtagcga
     6241 ctcatttttg tcatcaagat tcacccctta caaatataga taaagtctga ctttgtgcgc
     6301 ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa tggtaacgct
     6361 gcgtggcctt tccagaggat gcatctccag ccctctggcg tcataccagt aaaaacgata
     6421 atgaacggta acgggttctt gcctttcgtt ataaagcgtt gaggatgctg aaggttgaat
     6481 atcagacgtc gaaaggacgg gcttttctgc actgatgccc gcagccaata aagatgactc
     6541 catcaccagc gattgctcat cattcaccgg aatttccgga tgtgaacgac agcccaccag
     6601 cagtaacaac accagagaca ccagcccaaa gcatcctttt ctcatcgtta cagaccttta
     6661 tgcgccagca ttggtcccag cggacggcca cccaacaagt gcatatggat gtggtaaacc
     6721 tcttgtccgc catggcggtt ggtattcatg atcagacgat agccatcttc ggcaatacct
     6781 tcttgctcag caatttttgc cgctacggtg atcatgcgtc ccagcgcctg ctcatgctca
     6841 gctgacacgt cgttcacagt cggaatgagg atattcggaa tgatcagaat atgcgttggc
     6901 gcttgcggcg aaatatcgcg aaacgccgtt accagatcat cctggtagac gatatcggag
     6961 gggatctcac gacgaataat tttgctgaat atagtttctt ctgccacgac attttccttt
     7021 ttcaaatagc catgcgttgc gccatgctac gccgcgccac actgcgagta tagagtatgg
     7081 tcgagttaaa cctgctcttt caaccttaac tcacgatttc ttaagcaaaa aatcgttttg
     7141 ctgccgtata tatcgccatt attcccattt ctgcctgcga atgcgctgaa aagacattct
     7201 ctgtttggtt tatacgacaa atatgaatgc gtatatttct catttgcatt tacaaacaaa
     7261 attattcgca cactaaatat aaccgctcat caccctcctc cggcatgagc ctgacgacaa
     7321 cataaaccaa gagatttcaa atgctttcaa cacaatttaa cagggataat caatatcaag
     7381 ccatcaccaa accctcacta cttgccggtt gcatagcatt ggcactatta ccttctgccg
     7441 cttttgctgc accagccact gaagaaacgg tgattgttga gggttcagcc acagctccag
     7501 atgatggcga aaatgattac agcgtaacgt ctacctctgc gggtaccaaa atgcagatga
     7561 ctcaacgtga tattcctcag tcggtcacta ttgttagcca gcagcggatg gaagatcagc
     7621 agttacaaac gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa agtcaggcgg
     7681 attccgatcg tgctctttat tattcccgcg gattccagat cgataactat atggttgatg
     7741 gtatccccac ctattttgaa tcgcgctgga atctgggcga cgcactttct gatatggcac
     7801 tgtttgaacg cgtagaagta gtgcgtggcg cgacaggact catgaccggg acgggtaatc
     7861 catctgcggc aattaatatg gttcgaaaac acgcgaccag tcgtgaattt aaaggcgatg
     7921 tctcggcgga atacggtagc tggaacaaag aacggtatgt ggcggattta caaagcccac
     7981 tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat aacgactcat
     8041 ggctggaccg ctacaacagt gaaaagacct tcttctcggg cattgtcgat gctgatttag
     8101 gcgatcttac gacgctttca gccggttacg aatatcagcg cattgatgtt aatagcccta
     8161 cctggggcgg tttaccgcgc tggaatactg atggcagcag caacagttac gatcgtgcac
     8221 gcagtaccgc acctgactgg gcgtacaacg ataaagagat caacaaggtc tttatgaccc
     8281 tgaagcagcg gtttgctgat acctggcaag cgacactgaa tgccacccac tctgaagtcg
     8341 aatttgacag caaaatgatg tatgtcgatg cctatgtaaa caaagcggat ggtatgctgg
     8401 ttgggccata cagtaattat ggacctggct ttgattatgt cggcggcacc ggttggaaca
     8461 gtggcaaacg taaagttgat gcgctggatt tgttcgctga cggtatttat gaattgtttg
     8521 gtcgtcagca caatctaatg tttggtggca gttacagcaa acaaaacaat cgttacttca
     8581 gttcatgggc caacatcttc ccggatgaaa ttggcagttt ctacaacttt aatggcaatt
     8641 tcccacaaac cgactggtca ccacagagcc tggcacagga cgataccaca catatgaaat
     8701 cgttatatgc tgccactcga gtcacccttg ccgatccgct gcatctgatc ctcggcgcac
     8761 gttataccaa ctggcgggtt gatacgctga cttacagcat ggagaaaaac cacaccacgc
     8821 cttacgctgg tctggtgttt gacatcaatg acaactggtc gacctacgcc agctatacct
     8881 ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa atatctggct ccaatcaccg
     8941 gtaacaacta cgagctgggt ctgaaatcgg actggatgaa tagccgtctg accaccacgt
     9001 tagccatctt ccgtattgag caggataatg tcgctcagtc caccggtaca cctatccccg
     9061 gcagcaacgg cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa ggggtggaat
     9121 ttgaactcaa cggcgcaatt accgacaact ggcagctgac atttggcgca acgcgctata
     9181 ttgcagagga taacgaagga aacgccgtta atcctaatct gccacgcacc acggttaaaa
     9241 tgttcaccag ctatcggttg cctgtcatgc cagagttgac agtcggcggt ggtgttaact
     9301 ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg caccttccgc gccgagcaag
     9361 gtagctacgc gctggtggat ctcttcaccc gctaccaggt gacgaaaaac ttctcgttac
     9421 aggggaacgt caataacctg ttcgacaaaa cctacgatac caacgtggaa ggttctatcg
     9481 tctacggcgc accgcgtaat ttcagcatta ccggcacgta tcaattctga ataacacctg
     9541 taaaaaaggc agccatctgg ctgccttagt ctccccaacg tcttacagat tagtggttac
     9601 ggatgtactc atccatctcg gttttcaggt tgtcggattt agtaccgaaa atcgcctgaa
     9661 caccagaacc agcaaccact acgcccgctg cgcccagttt cttcaggcca gcctgatcca
     9721 ctttagacac atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg aggttagtaa
     9781 tgttttcttt accaccaaat gcagcaacca gagccggtgc catttcgctg gtacctgtcg
     9841 cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt gctttaatca
     9901 gcacgcggaa gatggtgtag taaacaatcg cataaccgat accgacgatc gggaacagcc
     9961 acagtttgct gctgttacca gacagaacga tgaagtcgat cagaccgtgc gagaacgacg
    10021 taccgtcacg catccccaga agaatacaga ttgggaatgc caggcctgcc agaatcgcgt
    10081 ggatgatgta caggatcggc gcaacgaaca tgaaggagaa ctcgatcggc tcggtgatac
    10141 cggtcaggaa cgaggtcagc gccgcggaga tcataatacc gcccactttc gcgcggtttt
    10201 ctggtttagc agagtgccag atagcaattg cggcagctgg cagaccgtac attttgaaca
    10261 ggaagccacc agacagttta cccgcagtcg ggtcacccgc catataacgc ggaatgtcgc
    10321 cgtggaaaac ctgacctgct gcgttggtgt attcaccaat ctgcatctgg aaaggtacgt
    10381 tccagatgtg gtgcagacca aacggtacca ggcaacgttc gatgaaaccg taaatgccaa
    10441 acgcaactac cgggttctgg taagcagccc actgagagaa ggtctggatt gcagaaccaa
    10501 tcggcggcca aatgaaggac agcacaacgc cagtaaagat ggcagccagg ccagaaatga
    10561 tcggcacaaa gcgtttaccg gcaaagaagc caagatactc aggcagctta atacggtaga
    10621 aacggttaaa catgtacgct gcgatcgcac cggagataat ccctccgagt acgccagtat
    10681 ccgccaggtg tttagaggcg atttcttcag caggtaaatg cagtaccagt ggcgcaacca
    10741 cggccatggt tttaaccatg atgccatagg caacaactgc ggccagcgcg gatacgccat
    10801 cgttattggt aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg tttgcaaaga
    10861 cggaaccgcc tgcttctgcc ataacatgcg atacaacggc gggcagccag ctgaaattcg
    10921 cggaaccgac gcccagcaga atacctgcga taggcagtac ggataccggc agcatcagcg
    10981 atttaccgac cttttgcagg ttagcaaatg cattcttaaa cataattgag agtgctcctg
    11041 agtatgggtg ctttttttac gttctcacgc gtggcaaggg gggagagcct cgccgtgtac
    11101 agggcatcta agcgcccttt atttattaca cagagtaaaa taattcagtg ccaatttgtt
    11161 tgacggctat cacgtttcaa cttcagatca ttggagaagt tgcacatttt gacaaaacat
    11221 atgtcacaat atttcaaacg tggttattca ccgcccaatt ttcggcggtg aactttactc
    11281 gttttagcca ttaattacga gctttaaaaa aactcattca acggatggat tgaaggcggg
    11341 aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc agttcttcaa
    11401 cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc gcaggttgat
    11461 tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc accagtaacc
    11521 gatccagggg gacataacgc gcagcatcgc gcagttgctc cgcattacgg aaggtcacaa
    11581 tgccggaaaa ggagatgtaa aatccgagat ccagtaattt gcccgccgtt tctctgtcct
    11641 ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc agaattgcca
    11701 gcgtatcggc gcgggcgtca cgggtatgga cgataaccgg cttgttcagt tcacgaccaa
    11761 tctggatatg atggatgaag gactcttgct gacgaacttt agtttccggt gtgtaataat
    11821 aatccagccc ggtttctccc agcgctacaa caccctcttc tgccgccaga cggcgtaaat
    11881 cttctacatc gtagggatca ttctggttga gtggatgcac gccacaagaa aatacgacgt
    11941 tgtcacgttc gcctaccaga tcccgcatgt gtaaatagcc cggtaatgtt gtggcgactg
    12001 ccagacaaaa attcacatcg cgtgcggcgg ctttcgccag aacgtcatcc acgtccttat
    12061 gcaaagattc ataatccaga ccatcgagat ggcagtgtga gtcgactaaa aacatgatgt
    12121 ctctcttaaa gatgaggaac cggtagcaca acgcccggtt gcaggtaatg ctcaatacgc
    12181 agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg tgacagacat taactgttca
    12241 cgaatgtggc aaacatcccc cagtatagcc tgcaggcgcg agggagaaag atggtttgcc
    12301 agttcgacga caaggcccgg cacatcaaca ttggtcacct gcgcagcacc atgatggcgt
    12361 tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc ttgttcatga
    12421 ttaagggccg ccagcagcga ataccagtcg cccgatggca cgctatatgc caatgcctga
    12481 cacaatgttt cacgagcctg ccagttatct ccctgaaaca acgccagtgc cgcgccaggc
    12541 gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt cacttcgcgt
    12601 gaaagccagg tcacggcgta ctgttccggc ggcggcgcaa ggtaatgtaa ccgacaacga
    12661 ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa ccaggtttct
    12721 gctggtggct cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt cagtaaggca
    12781 gcatcggtta cccagacaac tttcgcacca cctaagcgtg cgtgctcatt cagcttttcg
    12841 gtgacctcac gtaccgcatc aatgcccaaa gcatttttcc ctttttcggg agccagggtg
    12901 tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc cacgacagtg accgcaactt
    12961 ttgtgaccct gcggttgttg gcagagtaaa taacggctca gggcgtagat taaagcatca
    13021 tcgcccatgc ccggtaacgc ctgaatgagc agcgcatggt gacctcttcc ggcctgatag
    13081 ctggctacca gtttttcgaa atcaggtcgt aaccatggat accatctcat gcgtccaact
    13141 ccttcaccca gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc agcggctggg
    13201 tggcatcaat ggtatgaatg cttttatctt gtgctgccag ttccagatag cgggcgcggg
    13261 tgcgattaaa gaaatcgaaa gactcttgct caatacgatc cagctcgccg cgcgcacgcg
    13321 cgcgttttaa gccaacttcc ggggtaacat cgagatagag cgttaagtcg gggcgaaaat
    13381 ccccgagaac agcatcacgc agtgttgcca gcatatgttg gtcaatacca cgtcctccgc
    13441 cctgatacgc ctgagtggag agatcgtggc gatcgccaat cacccaggtg ccgttagcca
    13501 gcgcaggttt gatgactgtt tctaccagtt gaacgcgcgc ggcataaaac atcagaactt
    13561 cggctttatc ggtaatgact tcatcgccta ccgatttgat atccagcacc aggcttctta
    13621 acttttcggc aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg cggataccca
    13681 gttgctcgag cgtctcaacc accacattac gcgcggtagt tttgcctgcg ccttccagcc
    13741 cctcaatgac gatatactta ctgcgcattt ttttccttaa gcactttcag ataatcctgc
    13801 acagacttgt tatgactggc aagattggta ttaaacgtgt gaccaccttt accatcggcc
    13861 acaaaataga gatacggcgt ttttgccgga tgcgcagcag ccttcagcga atccgccccc
    13921 ggcgtagcta tcgcacccgg cggcaaaccg gtaatggtat aggtgttata cgctgtcggc
    13981 gtttccaggt ctgcacgaga aagtttgcca ttataacgct ctcccatccc gtaaatcacg
    14041 gttgggtcgg tctgcaagcg cataccaatg cgtaaacggt tgataaatac cgaggcaacc
    14101 tgatcgcgtt cactggcaac ggcggtttct ttttcgataa ttgatgccat cgtcaccagc
    14161 tgatttttat ctttataagg cagaccgtcc gcacgccctt cccaggcgct atcgaccgct
    14221 ttcaccattt tcttgtgcgc tcgcttgagt aacgcgacat cggtggtatt ggcggtatac
    14281 atccaggtgt ctggccagaa ccaaccttca atccactccg ggttttccag ttcaagtgcc
    14341 tgcgctacgg tggcgtactt atcgtcgctc agcgtatgct tgatatacgg ggcctcacgc
    14401 aattgcttga ggtaatcgct cagacgcatc ccttctacca gtcgcagagg gaactgtgct
    14461 tctttaccgc tttccagcaa cttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg
    14521 taagtcccgg ctttaaagtg agaaagatcc ggttcgatac gcagcagcca ttgaaaaacc
    14581 cgtgggcgat tgatgatctt atcggcataa agctgttcac cgagcgccag acgtccggtc
    14641 cctggcttca gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg
    14701 cgaaccttcc agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata
    14761 atcaataaca cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat
    14821 ataacgttgc tgacgaaaag gagacatcgc cacaggcacg tacgggcatc actggcatta
    14881 acgcattaca aataaccatc tcatctgcct gcaacgcctc ttccagagag gcttgcactt
    14941 cgacaagctg ataagaggat tgtgccagca aacggataca gaattgtcgc ataatgccgt
    15001 taacacctgc ctgatccagt cgcggcgtat aaactacatt gcccttccgc cagaacaaat
    15061 tagccgcaca gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt
    15121 ttgtctgctc aagatgagag cgaatcaata cttgctcaag tcgattaaga tgtttaatac
    15181 ttgcaagatg aggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccctcgt
    15241 tacgcaaacg gtcgtaatgt gcaggataag ccgtaacgga gagaatccgc gttgccggtc
    15301 cactgttcaa tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagta
    15361 caccattttg ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt
    15421 cacaggaaat catcaaccgc tgacaagcat catgtagtcg ctggatatgc gcagataaca
    15481 aactgacttt accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg
    15541 cccgatcgct tactgccagc gattcctgct tataaccgtt aattaagaac ataccagctc
    15601 cttatcagag aattgttagt gtggcagcat gttcactacg gaacaagtcg gaataaaagc
    15661 taagaaaaaa ggcccgcaag cggacctttt ataagggtgg aaaatgacaa cttagatctt
    15721 tttaaagatc aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc
    15781 cattccgctg acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccgg
    15841 gttatccagg ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat
    15901 agattctact gcacccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac
    15961 caacacacgg cttgcagctt caccgaagat ggttttcacc gcctgcgctt cagctttatc
    16021 gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc
    16081 tgcatcacgc agagcatttg ccattgccag agctgcgcct gcgccatttt ctggcggtga
    16141 cgtcatatga taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc
    16201 accgcgtttt ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac catcgcccag
    16261 tacgaagcca tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg
    16321 ggtagataat gcacgtgccg cgccaaaacc accaacgccc agcggcgtac tggctttctc
    16381 tgcgccacct gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatggcc
    16441 aatgttgtgc acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc
    16501 atacatgata gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg
    16561 gctgatctta cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag
    16621 gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatttc
    16681 aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc
    16741 catcttgcgc tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc
    16801 agcaaatttc gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg
    16861 accggcaagc agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc
    16921 cagtccggtc acaactacac gacgcttaga cacgtttgtc ctccagggag ggaaaaaatg
    16981 attctagtgg gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg agatgttcac
    17041 ttacgcctgg tggccgttga tgtaatcaat ggcagcctga acggtggtga ttttctcagc
    17101 ttcttcgtcc ggaatctcag tatcaaactc ttcttccaga gccattacca gctcaacggt
    17161 gtcaagagaa tccgcgccca ggtcttcaac gaaagaagca ttgttggtaa cttcttcctg
    17221 cttaacgccc agctgttcgc cgataatttt cttaacgcgt tcttcgatag tgctcatact
    17281 cttaaatttc ctatcaaaac tcgctttcgc gatggttttc gtagtgtata aaatgttgaa
    17341 aaatttgcaa ctaaatcccg gcaggtctta ccacgatttt acgttatttt gaggccttac
    17401 cccccaataa cgcaaatcat tttgcactaa ttattactca taaccacgca gactgcgcgc
    17461 aacatgagca ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt tcacatgcaa
    17521 agtttcaccc gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg cgttggcgat
    17581 ttcctgtgcg ccgccgaggc gacccgcagg aacctgcgcc aggatacccg cacgctggtc
    17641 atcgctcagc gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa cgtttacagt
    17701 aataccgcgt gacgcaactt cgcgcgccag tgatttactg aagccgatca agcccgcttt
    17761 cgccgcagcg tagttggcct gaccgccatt tcccatggta ccaaccacag aaccgatagt
    17821 gataatacga ccatgacgct ttttcatcat agcgcgcatt accgcttttg acagacggaa
    17881 aacagatgaa aggttggttt cgataatatc gttccactct tcatctttca ttcgcattaa
    17941 caggttatca cgagtgatac cggcattatt gaccaggata tccacttcac caaattccgc
    18001 gcgaattttt tccagaacag attcgataga tgccgggtcg gtcacattca acatcagacc
    18061 tttgccgtta gcacctaaat aatcactgat cgcctgagcg ccattttcac tggtcgcagt
    18121 gccaataact ttcgcgccac gggctgcgag cgtttcagca attgcgcggc caattccgcg
    18181 gcttgcaccg gttaccagtg cgatttttcc ttcaaaattc atgattttcc tcttttaaag
    18241 ctcgagcgcc gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca gggtgtcgac
    18301 aatgcgtttc gtcaggccag taagcacttt gcccgggccg acttcataga gatgttctac
    18361 gccttgcgct gccatgtact caacagactt cgtccactga accgggttat acaactgacg
    18421 taccagtgcg tcacggatgg catcaccatt ggtttcgcat ttcacatcaa cgttattcac
    18481 aacaggaact gttggtgcgt taaaggtgat tttcgctaat tctactgcca gtttgtcggc
    18541 tgctggtttc atcagcgcac agtgagacgg tacgctcact ggtaacggca gagcgcgttt
    18601 tgcgcccgca gctttacagg cagcgccagc acgctcaacc gcttctttat gaccggcaat
    18661 aaccacctgt cccggagagt taaagtttac cggagaaacg acctgacctt ctgcagcttc
    18721 ttcacacgct ttcgcaatag acgcatcatc cagaccgatg attgccgcca tagcgcccgt
    18781 gccttccggt acggcttctt gcatgaactt gccgcgcatc tcaaccagac gcaccgcatc
    18841 agcgaaatca atcacaccag cgcaaaccag cgcggagtat tcccccaggc tgtgaccggc
    18901 catcattgcc ggtgctttac cgccctgctg ctgccatacg cggtacagcg caacagatgc
    18961 agtcaacagc gctggctggg tttgccaggt tttattcagt tcttcagctg gcccctgctg
    19021 ggtcagcgcc cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg tttcttcgac
    19081 aattggatag ctcgccgcca tatcagccag cattccaacg gtttgagaac cctgtccagg
    19141 gaacacaaat gcaaattgcg tcatgtttta atccttatcc tagaaacgaa ccagcgcgga
    19201 gccccaggtg aatccaccgc caaaggcttc aagcagaacc aactgccccg gcttaatgcg
    19261 cccgtcgcgt acagcttcat ccagcgcgca cgggacagag gccgcagagg tattaccgtg
    19321 gcgatccagc gtcaccacga cattgtccat agacataccg agttttttcg ccgttgcact
    19381 gataatacgc aggttagcct gatgcggaac cagccagtcc agttgagaac ggtcaagatt
    19441 atttgccgcc agcgtctcat caacgatgtg cgccagttct gttaccgcaa ccttgaagac
    19501 ttcgttgccc gccatcgtca gatgaattga attctctgga ttcacacggt cagcattagg
    19561 cagcgtcagc aactcaccat agctaccgtc ggcatgcaga tgggtggaga tgattcccgg
    19621 ctcttcagag gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa taatagtccc
    19681 acgatcggtt ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca gagcatactt
    19741 caccgcccca gatttcacgt attgatcggc tacgcttaat gcataggtga aacctgcgca
    19801 ggctgctgca acgtcaaatg ccgggcaacc tttaatgccc agcatgcttt gaatctgaca
    19861 agctgcgctc gggaaagcgt gcgtagcaga agtcgttgcc acaacgatca ggccaatctg
    19921 gtctttctca atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc ccatggttga
    19981 aacggtttcg tttggcgcgg caatgtggcg ttcgcggata ccggtacgag tgacaatcca
    20041 ctcgtcagag gtgtccacca ttttttccaa atcggcgttt gtccgcactt gttcgggcag
    20101 atagctgcca gtaccaataa tcttcgtata catgtacgct cagtcacttt tcggttatat
    20161 accgtcactt gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga gttccgcttt
    20221 tgccaccgtc cagcagctca aaaccagctg ggtatacaga ttccaggcga gcggcaattc
    20281 gctgaggaac ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc gcaaaagctc
    20341 gctgattggc tgcaccatga ctttttatca ccgtgccgcg caatcctaac agacaggcgc
    20401 cgttatactg gtcggggttg aggtgactga atcgcctcgt caggctcttt tgtagccaac
    20461 gctttaataa cagtagccac cacgaccgtt ttttcccttc accctgagat ttcagcagag
    20521 aaaggaacat cctgacaaca ccctccatcg tctttaatgt gacatttcct gtaaagccgt
    20581 cacaaaccag cacatctgtc ttgccagtta acaactcatt ggcttcaaga tagccgatat
    20641 aattgataga agggattgtt ttaagcaccg ctgaggcatc ccgaatactg tcgagaccct
    20701 ttacttcttc ttcaccaata ttgagcaacg ccacgcgagg attgggaatt tccaccacct
    20761 cttcagccag aactgagccc ataatggcaa attgcaccaa cattgtactg tcacaatcga
    20821 cgttggcccc taagtcaagg accaccgttt tgcccttttg ctgatgtggt aataccgtca
    20881 ccagcgccgg acgctcaatc ccctccaggg gcttgagtaa taattttgcc agccccatca
    20941 gtgccccggt attaccggca ctgacacagg cttgcgctcg accttctttc accagctcca
    21001 gggccacgcg cattgaactc ccacgactgg cgcggatagc ttgcgaaggc cggacatcac
    21061 tggcgataac tgactgcgca ggaataatct gcagacgcga acgttgttca aagtcagctt
    21121 tagcaagtaa tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg agttgcgaat
    21181 tagaattcag tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa tcccctccca
    21241 tgacatctaa cgccagggtt agacgtgtca aggtatcgtc gccgcccggt ttggtctttc
    21301 cccagttgcc tggggaaaat cctcactaag cttcatcacg cagacgcgtg attacttagc
    21361 gatgaccttg cggccgcggt agtaaccgtc ggcagtgatg tggtgacgca ggtgtttttc
    21421 accagaagtt ttgtctacag acaggctggt gactgcggtc agcgcgtcat gggaacgacg
    21481 catgccacgt ttggaacggg ttggtttatt ctgttgtacg gccatggacc ttactcctca
    21541 attacggttt atccccgatc caacggggag caccaattac ttacgcttta agctggctaa
    21601 tacggcaaat gggtttggct tttgcgcttc ttcaggcagt tcaccaaaga ccatgtccgc
    21661 ttcggacact tcacagtgtt cagaatcatg caccggaact accggcaagg cgaggatgat
    21721 ttcatcttca accattgcaa gcagatcgat ttcaccgaat tcgttaacct caatcggttc
    21781 atacgcttcc ggcagtgctt cagcctgttc gtctgaacgc acaggactaa aacaatacgt
    21841 tgtgtagacc tgatgagtaa acggcttccc gcaacgctga cactcgagcg ttaccgtcac
    21901 cttcgcatcg ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg acatggagca
    21961 ttccacatca ctgtccacac tgactacgga ttcggcgacg cgctcaacct gatcaggggt
    22021 atagataccc tggtaatcaa ggcgtttttg agccgtacga accggatcga gagtcagggg
    22081 taattttacc ttttgcatag ggcgcgcata ttaactttgt aacgtcatag agtcaaagaa
    22141 aaaggcagcc tgtggtttcc ttttgccaat aattcgcaca cattgcgggt tactgcttta
    22201 ttttcgttca gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata gtttaaaatg
    22261 gctatcatca atacgctata tctgatggaa aaaaatatgc ctaaacttat tttagcctcc
    22321 acctcgccat ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga atgtgcagca
    22381 ccagaggtcg acgaaacccc acgcagcgac gaatcaccgc gacagttggt gcttcgactg
    22441 gcacaagaaa aagcgcaatc tctggcgtca cgttatccgg atcatttaat tattggttca
    22501 gaccaggtat gtgttcttga tggcgaaatc accggtaaac cgttaacgga agaaaatgcc
    22561 cgtctgcaat tacgcaaagc cagcggcaat atcgtcacct tctatactgg actggcgttg
    22621 tttaattcgg cgaatgggca tctacaaaca gaagtggagc cttttgacgt ccatttccgt
    22681 cacctgagcg aggcggagat tgataattac gtgcgtaagg agcatcccct gcactgcgcg
    22741 ggtagcttta agagtgaagg atttggcatt acgctgtttg agcgtttaga gggacgtgac
    22801 cctaacacgc tggttggttt accgctaatc gcgctgtgtc agatgttacg tcgggaaggg
    22861 aaaaacccgc tgatgggata gttgtttgcg ccggatgcgg ttacgtcgca tccggcaatc
    22921 ggtgcaggcg gcctgatgcg gcgtaaacgc cttatcaggc caaccgccca ttatcaggct
    22981 tatatgcttg tattagcgcg cgttacgcag cttttgcaaa caacgcttca aaccttcatc
    23041 catcggcgct tcgatacgca tcacctcacc ggtccccgga tgggtaaact tcaacgctgc
    23101 ggcgtgcagg aacagacgat ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc
    23161 acggtcaccg tagcgatcgt caaaggcaat cggatgaccc gcatactgtg tatgcacacg
    23221 gatctgatga gtacgcccgg ttaccgggct acaacgcacc agggtggcaa atgcatagcg
    23281 ttcttccact ttaaagcgtg tttccgacgg tttgccttcc tgactgacac gcacgatacg
    23341 ttcgccgctt tgcagaatat ttttcaataa cggcgcttga acgctcttca catgcgactg
    23401 ccactgactg cgcaccagcg ccaggtaatc tttttgcatt cctttttcac gtaattgctc
    23461 atgcagagaa cgcaacgccg agcgtttttt cgctaccagc aacacacctg aagtgtcccg
    23521 gtcgagacga tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc
    23581 gataacgccg aagcttaaac cactgccgcc atgtaccgcc gtaccggaag gtttattcag
    23641 caccaggata tgatcatctt catataagat gacgtccgcc agcgccgcca ccttttgcag
    23701 atgtggcgaa accgcctctt cttcccgctc agcaacgcga actggtggga tacgcacctc
    23761 atcacccgct tcgagtttat attcaggctt aatacgtttt ttgttcaccc gcacttcgcc
    23821 tttacgcaaa atacggtaaa tcatactttt tggtacgcct ttcaattggg tacgcaaaaa
    23881 gttatcgata cgttgccccg cttcgtcagc ggtgatagca acaattttta cggatggagt
    23941 ctctgttttc atggttggcg attctaatta gccaacagga ttcgcgccac tcatttttct
    24001 atgcttatat ttacttttgc accttattac ttcactgcgt gatcacttta ttgatggtta
    24061 ttaaaccaat caccagcaag aagtgaaaaa actgtgagta agcgggtgat aaatggtaaa
    24121 agtcatcttg ctataacaag gcttgcagtg gaataatgag gccgtttccg tgtccatcct
    24181 tgttaaaaca agaaatttta cggaataacc cattttgccc gaccgatcat ccacgcagca
    24241 atggcgtaag acgtattgat ctttcaggca gttagcgggc tgcgggttgc agtccttacc
    24301 ggtagatgga aatatttctg gagagtaata cccagtctgt ttctttgata attgcgctgt
    24361 ttttccgcat gaaaaacggg caaccgacac tctgcgcctc tttgagctga cgataaccgt
    24421 gaggttggcg acgcgactag acacgaggcc atcggttcac acccggaaag gcgttacttt
    24481 gcccgcagct tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa agaatgttaa
    24541 tcaacgcaac tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag cgtctgtatg
    24601 acctggatat cgaaagtcca gggcacgagc agaaaaaggc aaacatctac aaaggtaaaa
    24661 tcacccgcat tgaaccgagt ctggaagctg cttttgttga ttacggcgct gaacgtcacg
    24721 gtttcctccc actaaaagaa attgcccgcg aatatttccc tgctaactac agtgctcatg
    24781 gtcgtcccaa cattaaagat gtgttgcgtg aaggtcagga agtcattgtt cagatcgata
    24841 aagaagagcg cggcaacaaa ggcgcggcat taaccacctt tatcagtctg gcgggtagct
    24901 atctggttct gatgccgaac aacccgcgcg cgggtggcat ttctcgccgt atcgaaggcg
    24961 acgaccgtac cgaattaaaa gaagcactgg caagccttga actgcctgaa ggcatggggc
    25021 ttatcgtgcg caccgctggc gtcggcaaat ctgctgaggc tctgcaatgg gatttaagct
    25081 tccgtctgaa acactgggaa gccatcaaaa aagccgctga aagccgcccg gccccgttcc
    25141 tgattcatca ggagagcaac gtaatcgttc gcgctttccg cgattactta cgccaggaca
    25201 tcggcgaaat ccttatcgat aacccgaaag tgctcgaact ggcacgtcag catatcgctg
    25261 cattaggtcg cccggatttc agcagcaaaa tcaaactgta caccggcgag atcccgctgt
    25321 tcagccacta ccagatcgag tcgcagatcg agtccgcctt ccagcgtgaa gtccgtctgc
    25381 catccggtgg ttcgattgtt atcgacagta ccgaagcgtt aactgccatc gacatcaact
    25441 ccgcacgcgc gacccgcggc ggcgatatcg aagaaaccgc gtttaacacc aacctcgaag
    25501 ctgccgatga gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg attgttatcg
    25561 acttcatcga catgacgcct gtacgccacc agcgtgcggt agaaaaccgt ctgcgtgaag
    25621 cggtgcgtca ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt ggcctgctgg
    25681 aaatgtcccg tcagcgcctg agcccatcac tgggtgaatc cagtcatcac gtttgtccgc
    25741 gttgttctgg tactggcacc gtgcgtgaca acgaatcgct gtcgctctct attctgcgtc
    25801 tgatcgaaga agaagcgctg aaagagaaca cccaggaagt tcacgccatt gttcctgtgc
    25861 caatcgcttc ttacctgctg aatgaaaaac gttctgcggt gaatgcgatt gaaacgcgtc
    25921 aggacggtgt tcgctgcgtg attgtgccaa acgatcagat ggaaaccccg cactaccacg
    25981 tgttgcgcgt gcgcaaaggg gaagaaacgc cgaccttaag ctacatgctg ccgaagctgc
    26041 atgaagaagc gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag cgtcctgaac
    26101 aacctgcact ggcaaccttt gccatgccgg atgtgccgcc agcgccaacc ccagctgaac
    26161 ctgccgcgac tgtcgtagcc ccagcaccga aagctgcaac ggcaacacca gcagctcctg
    26221 cacaacctgg gctgttaagc cgcttcttcg gcgcactgaa agcgctgttc agcggtggtg
    26281 aagaagccaa accgaccgag caaccaacac cgaaagcaga agcaaaaccg gaacgtcaac
    26341 aggatcgtcg caagcctcgt cagaacaacc gccgtgaccg taatgagcgc cgcgacaccc
    26401 gtagcgaacg tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat cgtcgccagg
    26461 cacagcagca gactgccgag acgcgtgaga gccgtcagca agctgaggta acggaaaaag
    26521 cacgtaccac cgacgagcag caagcgccgc gtcgtgaacg tagccgccgc cgtaacgatg
    26581 ataaacgtca ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa tctgttcagg
    26641 aaaccgaaca ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag cgtcagctca
    26701 atcagaaagt gcgttacgag caaagtgtag ccgaagaagc ggtagtcgca ccggtggttg
    26761 aagaaactgc cgctgccgaa ccaattgttc aggaagctcc agctccacgc acagaactgg
    26821 tgaaagtccc gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa gagaacaatg
    26881 ctgataaccg tgacaacggt ggcatgccgc gtcgttctcg ccgctcgcct cgtcacctgc
    26941 gcgtgagtgg tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc cagtcgccaa
    27001 tgccgttgac cgtagcgtgc gcgtctccgg aactggcctc tggcaaagtc tggatccgct
    27061 atccaattgt acgtccgcaa gatgtacaag ttgaagagca gcgcgaacag gaagaagtac
    27121 aagtgcagcc gatggtgact gaggtccctg tcgccgccgc tgtcgaacct gttgttagcg
    27181 cgccagttgt tgaagaaatg gctgaagtcg tagaagcccc cgtaccggtt gccgaaccgc
    27241 aaccggaagt ggttgaaacg acgcatcctg aagtgattgc cgccgcggta actgaacagc
    27301 cgcaggtgat taccgagtct gatgttgccg tagcccagga agtcgcagaa cacgcagaac
    27361 cggtggttga accgcaggaa gagacggcag atattgaaga agttgccgaa actgctgagg
    27421 ttgtggttgc tgaacctgaa gttgttgctc aacctgccgc gccagtcgtc gctgaagtcg
    27481 cagcagaagt tgaaacggta gccgcggttg aaccagagat caccgttgag cataaccacg
    27541 ctaccgcgcc aatgacgcgc gctccggcac cggaatatgt tccggaggca ccgcgtcaca
    27601 gtgactggca gcgccctact tttgccttcg aaggtaaagg tgccgcaggt ggtcatacgg
    27661 caacgcatca tgcctctgcc gctcctgcgc gtccgcaacc tgttgagtaa taattagctc
    27721 aaagtaatca agccctggta actgccaggg cttttttatt tcatctttga atcaacatat
    27781 taaaattcat cgaatggcat ccttgctaac caacaatgca aaataggcaa aaaaatgccc
    27841 gccccggcga tcccagagca ggcagacaaa acatacccag tttcatgata tgttttaaag
    27901 cgagcttatt tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac
    27961 gatgcctgca atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca
    28021 tcaaccagat cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taatgaatcc
    28081 agcgactcca gttcgttcag ctgcgtgcct aattccgcgc gaacagtcag cacattgttc
    28141 agcgagtttt tcagtccgcg gttggtttta tctaacgccg cagcggcggt ttctttatcc
    28201 gcttcgctat ccgcgaccgg cgttttcagg gctgcgatgg cactatccag catggcaaaa
    28261 agattggttt cagaagcgct accgtctggt tccgctaccg cgttgctggt aatactgtcg
    28321 aaaattttgt cacccgtgtg ccctatcacc atcgaacgcg aagcatcgac ctgttgttta
    28381 atactttctg ctccaccgac gtatttccct ttctcttcgc taaacggcgc agtctctgtt
    28441 ttataaccgg caaaaatgta gcgcccgtta ccgtcagtgg tgttcgccag attcagcaac
    28501 tggtcacgaa gcccctgaat atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg
    28561 ccattgctgg cgtagacaat tttttcctga gcattctgga tagcagtggt gacctggcta
    28621 agtacactct cttccagtga cactttttga gtggcgaaag tacgtgccag cgtgtactgg
    28681 ctgttttgcg cctgtgcctg ggagagaact acggcttgtg atgctgcaat gggatcgtca
    28741 gaagggttaa cgactcgctt acccgtcgac atctgctcgc cgtacttcat ccattctgcc
    28801 tgagaattgg tgatgccgcg catgttttgc tggtacatca tctgggtact gaaacgcatt
    28861 ttatctcccc ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa
    28921 cctgcgcatt cgccaggtaa tactgctgaa aacgttgcag atttccgtac tcctcatcga
    28981 gattgacacc ggaaatcgac tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg
    29041 tggtgctact ggttttcaac gtcgcggttt tattaccgat atcactcact aacgaggcat
    29101 aagcgtcgtt aaaggatttc gcaccgccca ccgttttact gttgctttgc agatccagca
    29161 gggcctgacc gttgcggttg tcgctatcac ccgcatcttc ttcgctcgcc atcgcgattt
    29221 tcgcttcgtc ggtgattaat acatccatgt tgacgatggc gtcacttact ggtttcagcg
    29281 tgaagctgtc gttaacggca ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca
    29341 ctttaccgtt ggcatccggc gtcaccgtaa aagtggtatt gctggcaagg cgggtgacct
    29401 gccactgatt attatcgaac gagattttgt aatctgtcgc cagtaacgcg gaggcatcag
    29461 ttaccgtggc accgatcgca acgtcacctt tgtttttcgt gttttgcaga accgcgggct
    29521 taccgatagc aaagaaatct tcaccagcat cgccgttggc atcaaatccg gctttgtgtt
    29581 ggctgttgaa agcctcggca aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt
    29641 ccagatcctg agaacggaat gtcagaatgc cgcccagcga cccggtattc agtaatttct
    29701 ccgggatctc aatattgcct gccgtcccat caacgtaagc gacagtcgta cgagaagggt
    29761 cggcgctgga aggaactgcc gccagttgcc gcgccgtact tccctgaacc agtgagtaac
    29821 cattggccat cgtgatgtta taagtaccgc catcctgaac gctgacttct acgccaacaa
    29881 tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc
    29941 ctgccccctc gcctgtcagg cgcgagattt gatcgttcag gctggcaatt tgtttagcgt
    30001 aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc
    30061 gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg
    30121 cctggcgcgc tgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat
    30181 cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca
    30241 tctgctcata gcgggcggtc aggccgctac tttgcgtctg cgcagcacgt aactggttgg
    30301 taataaacgc atcatactca cgctgcacac cagaaacgta gacgccattg ccaacccagc
    30361 cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg
    30421 caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca
    30481 gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag ccttcaatta
    30541 cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat tatcaatgtt
    30601 catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgga tcatgttggt
    30661 gagtttacgg gcatagtgag gatcggtagc ataccccgcg tcctgtaggg cctgcgcccc
    30721 ctgctccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc
    30781 aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg ctttaacttt
    30841 cttcgcttcg ccgttttcat attcagtcgt ggtgatttca gtaactggcc ctttccagtt
    30901 gccagaggct ttgacaccaa acaggttata gctcggctcg ccgttttcgc ggcggatttg
    30961 ccgttgcccc caaccagatt ccagtgccgc ctgagcgagg atcaaatgat gtggcacacc
    31021 gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctttactgtc
    31081 acccggcagc gaatcatcgt agttacgtgg cacggccttt tgcaccagct gcgaaagcgc
    31141 ctgattttga taacgcacca cagtttcgag cgggaatttc atcggtgctg ctggcgtgga
    31201 ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag
    31261 acctttgccc gccgtcatct gttgggcaat ctgctggtca tacatactgg tatacaggcg
    31321 agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat
    31381 catctgcacg aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc
    31441 ttcgcccgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag
    31501 tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt
    31561 tgcattgatt gcagtatgga catcagatcc atcggcgtag cgcccagcgc attgagcgcg
    31621 cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg
    31681 cgtaaatcga tctgcgtttg tggagtaacc acagtctgtc caccaccaaa cggtgtatct
    31741 ggctggctga cattggcctg acgattaact gttactgaga gattcccctg cgctaccgcg
    31801 cagctgtcga gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact
    31861 actttagcgt cctgcggggt gacattaaca tgcatattct gaatatcggc aaggaagcgg
    31921 acctgggaac tgttgccact cggtacgcgc acctgaatag tccgcgcatc taacgcggtg
    31981 gcgctgccat atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa
    32041 tcttcgtcgt taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca
    32101 cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca gttggttaac ctgaacactg
    32161 ctaccgccag cggaggctcc tgcgccgcca accagaatat tgccctgcgc cagcgcatac
    32221 acctgactgt caacgccctt aagcggtgtc atcaacaacg tacctccacg caagcttttg
    32281 gcattaccca tggaagaaac caccacatcg atggtttgcc cctgacgtcc aaacggagga
    32341 agtgacgctg tcaccattac cgcagcgacg ttttttagct gcatattggt gcccgtcgga
    32401 acggtaattc ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg
    32461 gttgtctggt caccggtgcc atccagcccc accaccagac catagccaat cagtgagttt
    32521 tgccttaccc cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtcgtg
    32581 accagtagaa gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg
    32641 acaggttaag gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat
    32701 tgcctacgta ttcaatgcgc gcatccgcca cctgagtaga cggtacggta ttgctgccgc
    32761 tgatagtgcg tggattaacc acgccagaga agcgaataaa ttcggtaccc tgattaatgg
    32821 caatctgttt ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca
    32881 ccgtcaacgt gccgctaaag gtattgctgg cattggcccc gccctttccg ttgaacgtgt
    32941 taccaccgga ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg
    33001 gcacagtatc aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt
    33061 tgctggcgct gacgttctcc tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc
    33121 gacgatcttc aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag
    33181 aaccgttggc gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca
    33241 gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag caccaacaag ctggaaatag
    33301 cataagtatg cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg
    33361 aacctgccac cggttatgcc ttagagttgc gtcagttttt gcagcatctg atcggtggtg
    33421 gacaccgctt tactgttgat ttcgtaagcg cgttgcacct gaatcatatt gaccagttct
    33481 tccgccacgt tgacgttaga cgtttcaaca tacccttgat acagcagtcc cgcgccgttc
    33541 aggcccggcg tgctttcgtt cggtgcaccg gaggattgcg tttcggtgta gaggttttcg
    33601 ccaatgctct ccagcccggt atcattcata aaggtggtga gattgagctg cccaacctga
    33661 accggagctg cctggccttg ttgggttacg ctgaccacgc catcacgacc gatggtgata
    33721 cttaacgcat tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc
    33781 agctgcccgt tctgatccac ctggaaagag ccgtcacggg tataggctga tgagccatct
    33841 ggcaacatca cctggaaaaa gccctgccct ttaatcgcga catctttgct gttgttggtc
    33901 tgcgacaggt tgccctggct gtgtaaacgt tcagtggcga ccgggcgtac ccccgtgccg
    33961 atttgtaatc cggagggtaa ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg
    34021 gtttgataaa gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt agtactgacg
    34081 tttgccaggt tgttggcaat gacgtccata ttggtttgct gggcgtcaag gcccgttttg
    34141 gcgatccata atgaactgat cataaaatgt cctgtagtgg attagtgaca aagtgcatat
    34201 gtcttcttgc cggatgcggc gtaaacgcct tatccggcct acaagagctc gcaaaatcga
    34261 taaattgcga ttgcccccat agacccgata aacttgcgca tcgggcaact tatacttgtc
    34321 atgtatcctt tcaattaact catcgacagc agttggttgg cacggcctgc gttatcatcg
    34381 acgctgctga tcaccttcat ctgcatttca aaacgccgcg cgctggcaat catgtcgctc
    34441 attgccgcaa cggcattgac gttactgcct tccagaaccc ccgacatcac acgcaaggtt
    34501 ggatccgcct gcagtaccgg cccacgcgtg gcctgggttt ctgcgcttaa acgaaaaatg
    34561 ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca ccagtttaag acgccctact
    34621 ggcgcaaccg tatttgccgg atcgcccgga ttgagcgccg agattgtgcc atcggcagca
    34681 atagtgattt ccgccccttc cggcacagca attggcccag cctcgcctat caccggatgc
    34741 ccctgaattg tcagttgccc ggtgggatca acctgaatgc tgccattacg cgtataccct
    34801 tcgctgccgt cagcggactg cacggccagc cagccatcct gctgcaacgc aacgtccagc
    34861 gggcgcgagg tgtaatccat cttgccgggc gtcatgtctg cacccggtgt tgacgccgtt
    34921 accagcgtgc gcgtgggcag tgaaagccct tccaccggca ctgcacgcaa ggcgtttaac
    34981 tgcgcgcgaa aaccgggcgt agaggcattg gccagattac tggcggttac cgcctgttga
    35041 ttcagcgtct ggctggctgc tcccatcgcg gtataaattg cgtgatccat tgagctatcc
    35101 cgtcagcgat tagcgtaagt taaccagcgt gttgaggatc tggtcctggg ttttgatggt
    35161 ctgggcgtta gactgatagt tacgctgggc aacgatcata ttgaccagtt ctttactgag
    35221 atcgacgttg gacgcttcca acgcaccgtt ggtcagggtg ccaaagttgc ccgtcccggc
    35281 tgtccccaac agcgccacgc cagaagattg cgtcgcagac cagacgttgt cgccttcgga
    35341 tgccagacct tcgttgttgg caaagttcgc cagtacaatc tgccccagca gttgtgtttg
    35401 ttcgttggaa tagttgccga caaccgtacc gtcatcattg atttgataac tcaccagatc
    35461 gcccggtttg tagccattct gggtggttgc cacaatattg ttagcgccgg tattttgctg
    35521 catggagttg aggaagctca ggctaaacgt ggcgggatct gcgccgttaa ttgcgccagt
    35581 ggtaatattc tctgttggat ttgaggtcag aacgccattg gcattaaaca ccagcttcat
    35641 tgcaggctcg gctgtacctg ttggatcact gctatcctgg gtgtagacct gccagttatt
    35701 atccccggtc ttcacaaagt agacgctcat gtcatgagca ttaccctgac tgtcgaaaac
    35761 agtcaccgaa ccttttttgt tatagctatc cgcattgctg gcgctgaatg gcgtaacagt
    35821 aggaagcgga tcgctggaat tcaggttgat ctgcatcgat gccgtggtgg tagttttcgc
    35881 tgccatcagg gtattcggga tcgaaatatt agtcggattc gccccttgct gaatagtcgg
    35941 cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct tgcatattca ccaggttacg
    36001 gttttcatcc agcttaaatt gtccgttacg gctgtagaac accgaaccgt tgctgtctac
    36061 cagacggaaa aaaccgttct ggctgatagc aacgtccaga cctcgcccgg tgttggtggt
    36121 cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt acccccagtc ccactttcga
    36181 accggcaaac atatcggcaa aagaggccgt gcctgattta aagccgtagg tggcggagtt
    36241 ggcgatattg ttgccaataa catcgaggtt ggtggcggca gcgtttaatc cgctaaccgc
    36301 ttgagaaaag gccatgactg actcctgaaa tgtgaaggct taaattatct gccgtacttc
    36361 gtcgagggtg gtggtgccgt aagtgccgag atccagcgta ttaccgttgt tgccgcggat
    36421 cacaccctgc accagagcaa actgcagcgg ctgggcaacc aattgtgtac caccgttact
    36481 ggcgctaatc actacgttgt aagaaccgtt cggcgcagtg ctgccgtcag tcaacgtacc
    36541 gtcccaggtg aaactgtgga ctccggcggt cagttcacca atatcaatag tgcgcacaac
    36601 cgcgccattt ttatcggtga tggtggccgt aactttgtct gccgcctgtt gcagctcaac
    36661 accaaacggc gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg caagaacagt
    36721 ggtgccgggg atcatcacgc cgtaaccgat caggttactg gcctgtaacg actggctgtt
    36781 atcaatctgt ccggaaatag atccgagcgt ggtattgagt ttttcaatcc cgctgaccgt
    36841 gctgatttgt gccagttgcg acgtcagctc gttgttttcc attggattgg tcgggtcctg
    36901 gtttttcagc tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg cggcgttgct
    36961 gcccgtgagc gaactactgc tggtggtact gacgccggta tttgtcggat cggtggtggt
    37021 taccgcaatg gacatagctt tctcctttat tgaccgagcg taagggtttt cagcatcatg
    37081 cttttcaccg tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc cgacatggtg
    37141 ttaaccatct ccccgacgac atcaacgttc ggcattttta cgtagccctt tgcatccgcc
    37201 agcggattac ccggttcata aaccagtttg tccggggcct gactttctat aacatcggca
    37261 acctttacgc cgcctgtcgc agcacctggt gcagcgttaa cctggaatac cacctgtttt
    37321 gcccgatatg gctgtccatc aggaccggtc acgctatcag cattcgccag attactggcc
    37381 gccacgttca ggcgctggga ctgggcagtt aacgccgacc cggcgatatc aaaaatattc
    37441 agcagtgcca tccgttaatt tccgctctgt aaaacgttca tcatgccttt gatttgcccg
    37501 ctcaacgcgc taaggctcat ctggtattgc aggctgttat cggcaaactg ggtgcgttcg
    37561 cgatccatat cgacggtatt accgtcaagc gaaggctggt ccggaatacg gtattgcagt
    37621 tctgcggtag gaggcgtcag cgcctgcgcc ggaatgtgtt gcgttgaggt catcgtcagt
    37681 gcaaccacac tggttgcatc ccgtccacgt tgcatgactt ttttaagttc actggcaaaa
    37741 tcgatatcgc gcgcctgata actaggggta tcggcattgg cgatgtttgc tgccagcact
    37801 tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc
    37861 ttatcgagca tatctcctcc gcaggtatca aaattctgcc atcagcttaa atgcctttac
    37921 gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt tgatggtcat
    37981 tgtggacggg tacaattcac gttgtagaaa tagctggggg gcgaaaatgc tggcaataaa
    38041 acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta agtgcggcga gcaatctcac
    38101 atcgcaattg cacacctttt ttagcgccca gctcgcgggg gtaagtgatg aggttcgtgt
    38161 ttctattcgt acagcgccca atctactacc gccgtgcgag cagccattgc tttcgatgag
    38221 caataattcc cgcctgtggg gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg
    38281 atatttacag gttaatgtac aggccacagg aaattatgtg gttgccgcga tgcccattgc
    38341 gcggggagga aagctggaag ctggcaatgt caaactgaaa cgcggacggc tggataccct
    38401 gccaccgcgt acggtgctgg atatcaatca acttgttgat gccattagcc tgcgcgatct
    38461 atcacccgat caacctatcc agttaaccca gtttcgccag gcatggcggg taaaagcggg
    38521 acaacgcgtc aacgtgatcg ccagcggtga tgggtttagc gccaacgcag aaggtcaggc
    38581 gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc atggtatcgg gacaggtagt
    38641 cagcggcgtt gttgatgcag atgggaatat tcttataaac ctgtaagctg ttaaagatta
    38701 cccgtccctt gccgataaat aagcaacaca tgataaaagc gccctcaatg aggaataaac
    38761 catgagtatt gatcgcactt cgcctctgaa gcctgtaagc accgttcaac cgcgcgaaac
    38821 cactgacgcg ccggtaacga acagccgggc ggcaaaaaca accgcctcca ccagcaccag
    38881 tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc ggcagcagtg atatcaatct
    38941 tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt gaactaaaaa tggacaccgg
    39001 caaaattgcc gatgcgctga tcaacgaagc tcagcaagat ttgcagagta actgatcgta
    39061 tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg
    39121 taatggatca agagcagcaa catctctcta tggggcagat caacggcagc cagttgcaat
    39181 ggattacaga acaaaaaagc tcactgctgg cgacgctgga ttacctcgaa cagttacgca
    39241 agaaagaacc caatacagca aatagcgttg atattagtca acgctggcag gaaattactg
    39301 tgaaaacgca gcaactacgc caaatgaatc aacataacgg ctggttactg gaaggacaga
    39361 ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg
    39421 gggcgaacgg tcagacctca acaacccatc gcggcggtaa aaagatttcg atctgaatat
    39481 tatctccggc ctgcactgca ggccggaatg cattgttaca ccgtccggcg ggcaaattct
    39541 ttaactttga agcccagtac cgccagtgca gcgaagtacg cggcaatccc cgccagcacg
    39601 accgccatta aacgcagtaa gcgccagggc atggtaccca atgaccactc cggcatgata
    39661 tgtaacatac ctaaaagcac gccagacatc accagtaccg ccaccaccag acgcaacaga
    39721 aacgccatcc agccgggttg cggagtaaag attttctgct tacgcaactg ccagtaaagc
    39781 agcgaagcat tcagacacgc cgccagacca atagaaagtg acagcccggc atgtttcaac
    39841 ggaccaataa acgccaggtt catcaattgc gttaaaatca gcgtaacgat ggcaattttc
    39901 actggcgttt taatgtcctg gcgggaataa aagccaggag ccaacacttt cactacaatc
    39961 aggccaatca aacccaccga gtaggcaatt aacgcccgct gggtcatcag cgcatcaaac
    40021 gcggtaaatt taccgtactg gaacagcgaa acggttaacg gacctgaaag aatgcccaac
    40081 gcaaccgcac tcggcagcgc caacaggaaa caaagacgca acccccagtc catcaaacgg
    40141 ttgtattcat catgattgcc actggcaaaa cttttcgaca gcgatggcag caaaatggtg
    40201 ccaagcgcca cgcccagcac accggaggga aactccatta agcggtcggc gtaatacatc
    40261 caagacaccg aaccggaagc aagaaacgag gcaaaaatgg tgttgatgat taaggagatc
    40321 tggctcacag agacgccaag gatcgccggt cccatctgtt tcaccacgcg cattgctccg
    40381 gcatcgtgga agttaatgcg cggcaggacc agcatgccga tcttcttcag gtgcggtagc
    40441 tgatacacca actgcaggat gccgccgacc gtaacagccc acgccagcgc cagcaccggt
    40501 gggttaaagt acggtgcggc aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt
    40561 ggcgcaaacg ccggaatcga gaagcggttc cacgtattca gaatcgctcc caccagcgac
    40621 gccagggaga taagcaagat atagggaaag gtaatcttta gtagctggct ggtcagggca
    40681 aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca tgatcaccca cggtgcggcg
    40741 agcatgccag cgaccgtcac aaccgccagc gcaagtgtca gcaggccaga aacataagag
    40801 acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat attccgccag aatcggtaca
    40861 aatgcctggg aaaatgcccc ttcggcaaag atacggcgta acaagttagg aagtttaaaa
    40921 gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga ttctggcgac aattgcgtct
    40981 cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt
    41041 aataaattca tcggtgttct aatccagacc cacgggcaaa acgcctgcaa ggcaggcgct
    41101 gaaagaggcg aatagtctac ctggattatg gtgaattgct accgccagat gttacagggt
    41161 ttattcactc atcgcatcgc gccagatctt gtcaacgata cgttgcgcca gcactgcctg
    41221 ttcgccggcg gtttgcggaa ctgtctgatt ttgcacacat tcaatgaagt gccgcgcaca
    41281 gccgacaaat ccacgttgct caagagtgct ctgccaacca ggaatcggtt tatgcactac
    41341 gccctgcccg cgctcctcac gccattcgcg catatccgta atttcgatga gcgcaccgtc
    41401 agtcacggcc tgcacggttt cacgctgact tccggcacgg cgatgcatac aggtggtgat
    41461 ctgcaaagga ccagccgaaa aatggtgctc ggcaaacagc atttcgccag cgtcgttagt
    41521 cagtagcgta ccgccatcca gagaggcttt gccacccgac aaccacagcg cggtatccac
    41581 cacatgcaga taatcatcca gcaacgtgaa ataaagatcg tgtggcccga cgctattgct
    41641 acgatgttta tccattctta gcgaagctgc ggtggcgagt tgcgttttta actctccgta
    41701 gagtggtgcg aaacgacggt taaaaccgac catcaacgtc agttttttcc gcgccgccag
    41761 ttccaccagc cgttcagcat cgcgcagatt ttctgccagc ggtttatcga cacagacatg
    41821 tacccccgca ttgagtaacg tactgaccac gtcaaagtgg ctggcggtgc tggaatgcac
    41881 aaaaaccgca tcgcaactgg cggcaaggct ggataacgaa tcggcataag gaatgcgcca
    41941 gctttcacaa attggcaggg ctttcgcgcg cgtaggcgac caggctcctt gtaacatcca
    42001 gtcagacgct gccgccagca ccggtaacca cgctttttgc gcaatgccgc ctaatcccac
    42061 aacgccgata cgtaattttt tcactttaat ctcccagatg ggccagcaac gaatcaagac
    42121 gctgtttcag ttctgccact tcgatttcca gggcttcgac gcgggcctgt aaatcaccat
    42181 caaccgcgtt cgacatatcc gttaccgccg gctgatcctc tacctcacca ctgaaaagat
    42241 gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag acgcaccaca aaaggaccat
    42301 cttcgcgatt tgccagttgt tccagcgtcg actccacttc cgccatatcg ctgaattcat
    42361 acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg ggcaccacgc aataacaacg
    42421 tggtgattaa cgccacttcc gctgcgctca gtttcagatc gccaaattct gaattacaaa
    42481 aacgttgttc atatttggtg acccgattac caaagccact caccgtgcgc agataatggc
    42541 gtttgaccag attatccagt tgctcctgca cttcggattc actcagattc atgaccggtt
    42601 cacggttcgt tttctgatta caggccgtga ctacaccatt gactgagagc gggtattgct
    42661 ccggcgtggt cacctgcttt tccagcaagc aaccaatcac gcgagcttca agggcagtaa
    42721 gttgatattt catgagattc tccttagcgg ccgggcgtcc agtctggggt agttaatgcc
    42781 gtcagtacgt gatcgcgcca ttgtccatca atcaacagat agtctttcgc atagccttct
    42841 ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt tattgtgcgg catataatta
    42901 gccataatgc gatgaatatg ttgggtgcgc tgcatataac gaatggctgc ggtcagggct
    42961 tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg aataaccgag atagcaggca
    43021 tgaaaagagc cacgaacaac attggaaaaa ttggcaacgc caataatctc tttttcgtcc
    43081 gggtcgaata agccaaagta gaaagctgaa ccttgtttat gaaattcgtt aatcatcccc
    43141 agcctggcct gccagcctga tggataacag tggctttcgt cgcgcactgg ctcccagggc
    43201 ttgaggaaat ggcgattctc tgcgtaataa tccgcaagac gccaggcatc acgatcatgc
    43261 accagacgca cgaccagtcg gtctgtggtt aagcgcactt ttggcacgtt actgcgatag
    43321 ccaaacattc tatacctact ccttcccgta acgtctttaa tacgctgtaa gtattactat
    43381 acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat tttctgcatt attcacatat
    43441 ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa aatattgtcc ccgaagtgat
    43501 gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca tcttttattt cccgggaggg
    43561 gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat atttcctgct catcgataat
    43621 atgctggtcg tgctggggtt ctttgttgtc ttcccgctga tctctatccg cttcgttgat
    43681 caaatgggct gggccgccgt catggtcggt attgctctcg gtctacgcca gtttattcag
    43741 caaggtctgg gtattttcgg cggcgcaatt gccgaccgct ttggtgccaa accgatgatt
    43801 gttaccggta tgctgatgcg cgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg
    43861 tggctattgt ggttttcatg cctgctctcg ggactcggcg gcacgttgtt tgatccgccg
    43921 cgttcggcgc tggtggtgaa actaatccgt ccacaacaac gtggtcgttt tttctcgctg
    43981 ttgatgatgc aggacagtgc cggtgcggtc attggcgcat tgttggggag ctggctgttg
    44041 caatacgact ttcgcctggt ctgcgccaca ggggccgttt tgtttgtgct gtgtgcggcg
    44101 ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa
    44161 ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct atgtcctgac gctggcgggt
    44221 tactacatgc tggctgtaca agtgatgctg atgctgccaa ttatggtcaa cgacgtggct
    44281 ggcgcgccct ctgccgttaa atggatgtat gccattgaag cgtgtctgtc gttaacgttg
    44341 ctctacccta tcgcccgctg gagtgaaaag cattttcgtc tggaacaccg gttgatggct
    44401 gggctgttga taatgtcatt aagcatgatg ccggtgggca tggtcagcgg cctgcaacaa
    44461 cttttcaccc tgatttgtct gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa
    44521 accttaagtg cttcgctggc ggacgcaaga gctcgcggca gctatatggg gtttagccgt
    44581 ctgggtctgg cgattggcgg cgctattggt tatatcggtg gtggctggct gtttgacctg
    44641 ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc tgggaattat tggcatcttc
    44701 actttccttg cgctgggttg gcagtttagt cagaaacgcg ccgcgcgtcg tttgctggaa
    44761 cgcgacgcct gatttgatac tcccccctga ctcctccata ctgaaagtga actgacagcc
    44821 aaatggagga gtcacgtgaa gctatacatt tacgatcact gcccttactg cctcaaagcc
    44881 cgcatgattt tcggcctgaa aaatatcccc gtcgaattac atgttctgct caacgacgac
    44941 gcagaaacac ccacccggat ggtcggtcaa aaacaggttc ccattctgca aaaagatgac
    45001 agccgctata tgccagaaag catggatatc gttcactatg tcgataaact cgacggcaaa
    45061 ccgttactga ccggcaaacg ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc
    45121 tacgccaaca aactgctgtt gccgcgtttt gccaaatcgg catttgatga gttttctact
    45181 cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac
    45241 ctgctggccc actctgacgg tctgattaag aatatcagcg atgatttgcg tgcgctggac
    45301 aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt cggaagatga tattcagcta
    45361 tttccgctac tgcgtaatct gacgctggta gccggaatca actggccaag ccgcgttgct
    45421 gattaccgcg ataatatggc gaaacagaca caaatcaatt tgttatcatc aatggcgatt
    45481 taatcctgac ccgccgcggc agcctgtatt cgcgggttgc cgccgttttc agttttttct
    45541 gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt caggatattg acgcagattt
    45601 catgtcgagg actaccatga acaagttttt atttgccgct gcattgatcg tcagtggcct
    45661 gctcgttggc tgtaatcaac tcacccaata caccatcacc gaacaagaaa ttaaccagtc
    45721 gcttgcgaaa cataataatt tctcaaaaga tatcggttta cccggcgtgg ctgacgccca
    45781 tattgttctg acaaacttga ccagccaaat tggtcgcgaa gagccgaata aggttaccct
    45841 aaccggagac gccaatctgg acatgaactc cctgttcggt agtcagaaag cgaccatgaa
    45901 actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa ggtgcgatct tcctgaaaga
    45961 gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg caaacggtga tgcaaacgtt
    46021 gcttccctat ttgaaccagg cattacgcaa ttactttaac cagcaacctg cttacgtcct
    46081 gcgcgaagat ggcagtcagg gcgaagcaat ggcgaaaaaa ctggcgaaag gcattgaagt
    46141 gaagccaggc gaaattgtca ttccgtttac ggattaatga cgagggcgca ttcgcgccct
    46201 ttatttttcg tgcaaaggaa aacgtttccg cttatccttt gtgtccggca aaaacatccc
    46261 ttcagccgga gcatagagat taatgactgc accatcccag gtattaaaga tccgccgccc
    46321 agacgactgg caccttcacc tccgcgatgg cgacatgtta aaaactgtcg tgccgtatac
    46381 cagcgaaatt tatggacggg ctattgtaat gcccaatctg gctccgcccg tgaccactgt
    46441 tgaggctgcc gtggcgtatc gccagcgtat tcttgatgcc gtacctgccg ggcacaattt
    46501 caccccattg atgacctgtt atttaacaga ttcgctggat cctaatgagc tggagcgcgg
    46561 atttaacgaa ggcgtgttca ccgctgcaaa actttatccg gcaaacgcaa ccactaactc
    46621 cagccacggc gtgacgtcaa ttgacgcaat catgccggta cttgagcgca tggaaaaaat
    46681 cggtatgccg ctactggtgc atggtgaagt gacacatgca gatatcgaca tttttgatcg
    46741 tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc cagcgcctga ctgcgctgaa
    46801 agtcgttttt gagcacatca ccaccaaaga tgctgccgac tatgtccgtg acggaaatga
    46861 acggctggct gccaccatca ctccgcagca tctgatgttt aaccgcaacc atatgctggt
    46921 tggtggcgtg cgtccgcacc tgtattgttt acccatcctc aaacgcaata ttcaccaaca
    46981 ggcattgcgt gaactggtcg ccagcggttt taatcgagta ttcctcggta cggattctgc
    47041 gccacatgca cgtcatcgca aagagagcag ttgcggctgc gcgggctgct tcaacgcccc
    47101 aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg aatgctctgc agcactttga
    47161 agcattctgt tctgtaaacg gcccgcagtt ctatggcttg ccggtcaacg acacattcat
    47221 cgaactggta cgtgaagagc aacaggttgc tgaaagcatc gcactgactg atgacaccct
    47281 ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt aaacaataaa aatcgtagct
    47341 tcctgttgtc attaggttat tttacctgta taaataacca gtatattcaa cagggggcta
    47401 ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt gccagctggg gctattgacg
    47461 ccctggctgg cgaactttcc cgccgtattc agtatgcgtt tcctgataat gaaggccacg
    47521 tatcggtacg ttatgccgca gcgaataatt tatcggttat tggcgcaaca aaagaagata
    47581 aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag cgcc
//
LOCUS       DAOFQD010000022        46192 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.022, whole genome shotgun sequence.
ACCESSION   DAOFQD010000022 DAOFQD010000000
VERSION     DAOFQD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.022"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1476)
                     /locus_tag="QBF46_001320"
     CDS             complement(<1..1476)
                     /locus_tag="QBF46_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDQ6673523.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALA"
     gene            complement(1563..2213)
                     /gene="hxpA"
                     /locus_tag="QBF46_001321"
     CDS             complement(1563..2213)
                     /gene="hxpA"
                     /locus_tag="QBF46_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDQ6673524.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(2224..2718)
                     /locus_tag="QBF46_001322"
     CDS             complement(2224..2718)
                     /locus_tag="QBF46_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDQ6673525.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(2801..3256)
                     /locus_tag="QBF46_001323"
     CDS             complement(2801..3256)
                     /locus_tag="QBF46_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDQ6673526.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            3594..4796
                     /gene="ackA"
                     /locus_tag="QBF46_001324"
     CDS             3594..4796
                     /gene="ackA"
                     /locus_tag="QBF46_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDQ6673527.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            4871..7015
                     /gene="pta"
                     /locus_tag="QBF46_001325"
     CDS             4871..7015
                     /gene="pta"
                     /locus_tag="QBF46_001325"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDQ6673528.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            7205..8725
                     /gene="yfcC"
                     /locus_tag="QBF46_001326"
     CDS             7205..8725
                     /gene="yfcC"
                     /locus_tag="QBF46_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDQ6673529.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(8758..9300)
                     /gene="yfcD"
                     /locus_tag="QBF46_001327"
     CDS             complement(8758..9300)
                     /gene="yfcD"
                     /locus_tag="QBF46_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDQ6673530.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(9358..9909)
                     /gene="yfcE"
                     /locus_tag="QBF46_001328"
     CDS             complement(9358..9909)
                     /gene="yfcE"
                     /locus_tag="QBF46_001328"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDQ6673531.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(9965..10609)
                     /gene="yfcF"
                     /locus_tag="QBF46_001329"
     CDS             complement(9965..10609)
                     /gene="yfcF"
                     /locus_tag="QBF46_001329"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDQ6673532.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIVEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            10745..11392
                     /gene="yfcG"
                     /locus_tag="QBF46_001330"
     CDS             10745..11392
                     /gene="yfcG"
                     /locus_tag="QBF46_001330"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDQ6673533.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            11449..11811
                     /gene="folX"
                     /locus_tag="QBF46_001331"
     CDS             11449..11811
                     /gene="folX"
                     /locus_tag="QBF46_001331"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDQ6673534.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            11832..12725
                     /locus_tag="QBF46_001332"
     CDS             11832..12725
                     /locus_tag="QBF46_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDQ6673535.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(12773..13663)
                     /gene="rpnB"
                     /locus_tag="QBF46_001333"
                     /pseudo
     CDS             complement(12773..13663)
                     /gene="rpnB"
                     /locus_tag="QBF46_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
     gene            complement(13860..14633)
                     /gene="hisP"
                     /locus_tag="QBF46_001334"
     CDS             complement(13860..14633)
                     /gene="hisP"
                     /locus_tag="QBF46_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDQ6673536.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(14641..15357)
                     /locus_tag="QBF46_001335"
     CDS             complement(14641..15357)
                     /locus_tag="QBF46_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6673537.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(15354..16040)
                     /gene="hisQ"
                     /locus_tag="QBF46_001336"
     CDS             complement(15354..16040)
                     /gene="hisQ"
                     /locus_tag="QBF46_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDQ6673538.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(16130..16912)
                     /gene="hisJ"
                     /locus_tag="QBF46_001337"
     CDS             complement(16130..16912)
                     /gene="hisJ"
                     /locus_tag="QBF46_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDQ6673539.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(17133..17915)
                     /gene="argT"
                     /locus_tag="QBF46_001338"
     CDS             complement(17133..17915)
                     /gene="argT"
                     /locus_tag="QBF46_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDQ6673540.1"
                     /translation="MKKSILALSLLVGLSTAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(18181..18750)
                     /gene="ubiX"
                     /locus_tag="QBF46_001339"
     CDS             complement(18181..18750)
                     /gene="ubiX"
                     /locus_tag="QBF46_001339"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDQ6673541.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(18845..20362)
                     /gene="purF"
                     /locus_tag="QBF46_001340"
     CDS             complement(18845..20362)
                     /gene="purF"
                     /locus_tag="QBF46_001340"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDQ6673542.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(20399..20887)
                     /gene="cvpA"
                     /locus_tag="QBF46_001341"
     CDS             complement(20399..20887)
                     /gene="cvpA"
                     /locus_tag="QBF46_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDQ6673543.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(21146..21808)
                     /gene="dedD"
                     /locus_tag="QBF46_001342"
     CDS             complement(21146..21808)
                     /gene="dedD"
                     /locus_tag="QBF46_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDQ6673544.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(21798..23066)
                     /gene="folC"
                     /locus_tag="QBF46_001343"
     CDS             complement(21798..23066)
                     /gene="folC"
                     /locus_tag="QBF46_001343"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDQ6673545.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(23136..24050)
                     /gene="accD"
                     /locus_tag="QBF46_001344"
     CDS             complement(23136..24050)
                     /gene="accD"
                     /locus_tag="QBF46_001344"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDQ6673546.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(24206..24865)
                     /locus_tag="QBF46_001345"
     CDS             complement(24206..24865)
                     /locus_tag="QBF46_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6673547.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(24948..25760)
                     /gene="truA"
                     /locus_tag="QBF46_001346"
     CDS             complement(24948..25760)
                     /gene="truA"
                     /locus_tag="QBF46_001346"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDQ6673548.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(25760..26773)
                     /locus_tag="QBF46_001347"
     CDS             complement(25760..26773)
                     /locus_tag="QBF46_001347"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDQ6673549.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(26839..27975)
                     /gene="pdxB"
                     /locus_tag="QBF46_001348"
     CDS             complement(26839..27975)
                     /gene="pdxB"
                     /locus_tag="QBF46_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDQ6673550.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLYDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            28074..29069
                     /gene="flk"
                     /locus_tag="QBF46_001349"
     CDS             28074..29069
                     /gene="flk"
                     /locus_tag="QBF46_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDQ6673551.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGTGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(29066..30244)
                     /locus_tag="QBF46_001350"
     CDS             complement(29066..30244)
                     /locus_tag="QBF46_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6673552.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(30537..31757)
                     /gene="fabB"
                     /locus_tag="QBF46_001351"
     CDS             complement(30537..31757)
                     /gene="fabB"
                     /locus_tag="QBF46_001351"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDQ6673553.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            31916..33922
                     /gene="mnmC"
                     /locus_tag="QBF46_001352"
     CDS             31916..33922
                     /gene="mnmC"
                     /locus_tag="QBF46_001352"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDQ6673554.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQARLQRLHFISFEK
                     FPLTRADLAIAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(34043..34321)
                     /locus_tag="QBF46_001353"
     CDS             complement(34043..34321)
                     /locus_tag="QBF46_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDQ6673555.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(34355..34903)
                     /gene="epmC"
                     /locus_tag="QBF46_001354"
     CDS             complement(34355..34903)
                     /gene="epmC"
                     /locus_tag="QBF46_001354"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDQ6673556.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(34903..35712)
                     /locus_tag="QBF46_001355"
     CDS             complement(34903..35712)
                     /locus_tag="QBF46_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDQ6673557.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(35712..36536)
                     /gene="mepA"
                     /locus_tag="QBF46_001356"
     CDS             complement(35712..36536)
                     /gene="mepA"
                     /locus_tag="QBF46_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDQ6673558.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(36540..37625)
                     /gene="aroC"
                     /locus_tag="QBF46_001357"
     CDS             complement(36540..37625)
                     /gene="aroC"
                     /locus_tag="QBF46_001357"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDQ6673559.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLDIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(37660..38592)
                     /gene="prmB"
                     /locus_tag="QBF46_001358"
     CDS             complement(37660..38592)
                     /gene="prmB"
                     /locus_tag="QBF46_001358"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDQ6673560.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            38758..39309
                     /gene="smrB"
                     /locus_tag="QBF46_001359"
     CDS             38758..39309
                     /gene="smrB"
                     /locus_tag="QBF46_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDQ6673561.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(39381..40238)
                     /locus_tag="QBF46_001360"
     CDS             complement(39381..40238)
                     /locus_tag="QBF46_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDQ6673562.1"
                     /translation="MKILRYVFYFLLLICLSTGVQAAKKIATLYIPAGSMGVLTGFQF
                     NLSILTPEGAQYGVYQSRSVAILNVPLVSWTGQGTAPELHLEDARTILPTSRCPGLEE
                     YDARTPQTTWACVEMKVTVFYDGDMHGCPWIVSSYVKSEDATEAIYTGNNVPYIGPTK
                     VSSDCSAIPLDPYDVSWNENTVVHNKIVRMQSTGAVIEQTLPTYLMEGGKLCDGSKYD
                     ERGAYCRFVAQQMTFSTSGCDDAKVTVTPEPQPITSRQLHDMKLRVDTTAQQPIDATC
                     RFTYILNMY"
     gene            complement(40240..40779)
                     /locus_tag="QBF46_001361"
     CDS             complement(40240..40779)
                     /locus_tag="QBF46_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6673563.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLIVAGQSVHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGEFTASLTATFEYI"
     gene            complement(40776..41264)
                     /locus_tag="QBF46_001362"
     CDS             complement(40776..41264)
                     /locus_tag="QBF46_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6673564.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQEVDYELSCESDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            complement(41261..41770)
                     /locus_tag="QBF46_001363"
     CDS             complement(41261..41770)
                     /locus_tag="QBF46_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6673565.1"
                     /translation="MSHKFLAGAGLLVLAGWGVSGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVEFGNVVINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(41786..42538)
                     /locus_tag="QBF46_001364"
     CDS             complement(41786..42538)
                     /locus_tag="QBF46_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDQ6673566.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNNCQVDEEQSRKG"
     gene            complement(42558..45203)
                     /gene="yfcU"
                     /locus_tag="QBF46_001365"
     CDS             complement(42558..45203)
                     /gene="yfcU"
                     /locus_tag="QBF46_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HDQ6673567.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTWPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDGDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNMSVSLTGY
                     RYEYDNRADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFTVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVTSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(45285..45848)
                     /locus_tag="QBF46_001366"
     CDS             complement(45285..45848)
                     /locus_tag="QBF46_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6673568.1"
                     /translation="MSKFVKTAIAAAMVMGAFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11232 a        12528 c        11566 g        10866 t
ORIGIN      
        1 cgccagcgcg acacctcccg ttttctgtaa tgccacagca aacggcatca tcccaacgat
       61 caaaataatg ctcggccagt gaatggattt ataggcgctt tcagcatcta tacagcggaa
      121 tttccccatc agcaggcagg cgatgatagc ggcaacagga ttaggaattt catctgtcag
      181 cattaacgcc accattagca ccagacagaa aatggcatgg ggtgcctggc tgtgcgcggg
      241 cgatgcttca ctcacctctt ccggtaagtt cagcgctacg aagtcgcggc cctgtttggc
      301 cagcataccg atcagtttcc agttacccac aaccagaatg atatcgccca gcagcagagg
      361 ctcatccgcc agcgaacctt ccagcgccac gccattgcgc tttagcccca ccacattcag
      421 tccgtagcgg gtacgaaaac caatttcgcg caccgattta ccaatcagtt ctgactcagg
      481 aattaatgaa atctctgcca tgcccacatc aagggcctgg tcagaaaaat actcgccgcg
      541 cagtaccatc ggctccagca attgctcact acaaaattgc cggagatcga catcagccgc
      601 agacatatca ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat taacgttcac
      661 gataacgcga cgaaaacgcc gccagcgttc aacaccgatg acgttagcgc cataacgctc
      721 acgtaatttg agatcatcca gccgttgacc aatcattggc gatccggggc gaatagccag
      781 acgtcgcgca cgcccggtca gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt
      841 ccagccttcg cgctgcgggg tctgggtatc ccctttcagc atgaaacgca tcactaacat
      901 atacaaaata cccagcacca gcacgaccag gccaataggt gttacgctaa agaaactgaa
      961 gccgtgatag ccttcacgca gcaattcact gttgactacc aggttcggcg gcgtcgccac
     1021 cagcgtcatc atgccgctta tcagcccggc aaaactcaac ggcatcatca gacgtgacgg
     1081 cgaagtttgc atacgcatgg caacgcttaa caccacggga ataaagatag cgacaacgcc
     1141 ggttgaactc ataaacgcgc caagccccgc gacggtcagc atcaacaaaa ccaacatttt
     1201 gatttcactg ttgcccgcaa ctttgaccag ccatgttccc attacggtgg caacaccggt
     1261 acggaccaaa ccatcgccaa taataaacaa ggcggcaatc aggacaacgt taggatcaga
     1321 aaagccggaa aatacttcgg ggactgtcag cgttccgctt aatgcaaacg cgacaataac
     1381 aaacaaagcg accgcatcca tacgcactct gcccgtcgca aacaagacaa tggcaacggc
     1441 cagtaatgaa agaacccaaa tcaattcacc gttcacaact tatccttgtt aattgaggga
     1501 gatgtcttga ttctgccata aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg
     1561 tttcactgaa taataacatc gccatttggc tgtttggtca cagtaatttg ctccagactg
     1621 tggaggacca aatcgacctc attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg
     1681 acatgacaac ccgccgccag gccagaaagc acgccagcgg gagcatcttc caccaccaca
     1741 cactcctgcg gcgcaagccc cagcagctgc gcgcctaaca gatacgcatc aggttctggt
     1801 tttccgcgct tcactcgctc agcggttaca aacacctctg gtgcgggaag cccagctatt
     1861 ttatggcgcg ctcgcgctac cggcatggag ccagaagtca caatggccca cggaatacct
     1921 gctttattca aatgactgag taaggcgatt gcccccggaa gcgcggtaat accttcggtt
     1981 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
     2041 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
     2101 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
     2161 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
     2221 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
     2281 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
     2341 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
     2401 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
     2461 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
     2521 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccag
     2581 ttcacgcatc tgtaatccat aaccacgctc aatgattgtt tgcaggcgac ggtaacgttc
     2641 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
     2701 cgcgttggtc atttccattg ttgactcctg tatcactcta ttacggtgaa aaaaaagaag
     2761 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
     2821 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
     2881 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
     2941 accagttgag gatagcaggg ggtaatggcg ttacagaacg cttgcccagc caccataatc
     3001 cctgcatggg taaacttaag gcgaacagcg cagtggcaac tgccggccca agctgaccgc
     3061 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
     3121 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagcg
     3181 gccacgtctt tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta acagaacgat
     3241 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
     3301 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
     3361 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
     3421 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
     3481 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
     3541 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
     3601 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgca atcatcgatg
     3661 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cccgaagcac
     3721 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
     3781 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
     3841 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
     3901 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
     3961 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
     4021 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
     4081 tctacgccct gccgtacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
     4141 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
     4201 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
     4261 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
     4321 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
     4381 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
     4441 ccagcgactg ccgctatgtt gaagacaact acgcgacaaa agaagacgcg aagcgcgcaa
     4501 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
     4561 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
     4621 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
     4681 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
     4741 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
     4801 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
     4861 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
     4921 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
     4981 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
     5041 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
     5101 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
     5161 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
     5221 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
     5281 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
     5341 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
     5401 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
     5461 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagtc cgctgccggt
     5521 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
     5581 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
     5641 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
     5701 ggtgacttcc gcagaccgtc ctgacgtgct ggtcgccgct tgcctggcag ccatgaacgg
     5761 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
     5821 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
     5881 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
     5941 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
     6001 gactgccact tctgagcgca gccgtcgtct gtctccgcca gcgttccgtt atcagctgac
     6061 tgaactggcg cgcaaagcgg gcaaacgtat cgttctgccg gaaggtgacg aaccgcgtac
     6121 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
     6181 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactaggtg caggcattga
     6241 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg agctgcgtaa
     6301 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
     6361 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
     6421 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
     6481 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
     6541 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
     6601 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
     6661 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
     6721 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
     6781 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgttcatct tcccggatct
     6841 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
     6901 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
     6961 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaatctcg
     7021 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
     7081 ttttattctc ttaatttgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
     7141 atgaattgcg ccatctcact ttgcatactt accactttat tttgtgcaag ggaatatttg
     7201 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
     7261 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
     7321 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
     7381 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
     7441 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
     7501 attaacctca ggatcgaaat acgggacagc cgttggcatc atcatgttta tgctggtgat
     7561 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
     7621 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
     7681 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
     7741 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
     7801 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
     7861 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
     7921 cactctgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
     7981 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggctg atgttgaaca
     8041 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
     8101 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
     8161 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
     8221 taataccatg gcttcatcct ttaccgaagg ggcgcgaatg atgatcgccc ctgccctgct
     8281 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
     8341 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
     8401 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
     8461 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
     8521 ccgtcaggtt accgtgctgg cgttccagtt tggtgatggc ttcagtcaca tcatttaccc
     8581 aacctcagcg tcgttaatgg caacgctcgg tgtgtgcagg gtggacttcc gtaactggct
     8641 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
     8701 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
     8761 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgctag
     8821 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
     8881 ccagcaaact tcactgactt catcttcctg tagtgcgaag ggaccgtgag agacgcagct
     8941 gaacaatgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
     9001 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
     9061 ttgctcatcg gcctggacta caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
     9121 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
     9181 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
     9241 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
     9301 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
     9361 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
     9421 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
     9481 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
     9541 caccagcaca tcgttctggt ttaaagcagg cagattttcc gggccaaaaa gatgaccatg
     9601 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
     9661 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
     9721 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
     9781 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
     9841 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
     9901 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
     9961 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    10021 actggaatgt tgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    10081 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    10141 gacccagtac taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    10201 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    10261 ggatgggcat cagatcgctg cgcaaccagg cctgaatctg tcgtgcacgt gcacgatttt
    10321 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    10381 ccacaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    10441 gtgtctgacc gtaaccttgc cacgtcggct gcaaatgttc accgctgtcg aggtcgatgg
    10501 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    10561 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    10621 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    10681 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca tatgattaaa caaccggagc
    10741 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    10801 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    10861 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    10921 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    10981 ggctgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    11041 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    11101 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    11161 tctttaccat gtactgaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    11221 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    11281 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    11341 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    11401 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    11461 gccgctatta ttcgtataaa gaaccttcgt ttgcgtacgt ttatcggaat taaggaagaa
    11521 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    11581 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    11641 attcagcatg tagagaataa ccgtttctct ttgctggaaa aattaactca ggatgtgctc
    11701 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactacac
    11761 gcgctgcgct acgccgattc ggtatccatg accttgagct ggcagcgtta accgccatat
    11821 cgggaggctg catgaatata gtgatcaccg gagggacagg attaattggt cgccatttaa
    11881 tcccgcgttt gctggagttg ggccatcaaa tcacggtagt gacgcgtaac ccgcagaaag
    11941 ccagttccgt tctcggccct cgggtgacac tatggcaagg gcttgccgat caaagcaacc
    12001 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    12061 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    12121 atttgattaa tgccagcgac acgccaccgt cggtactcat ttccggctcg gcaacgggct
    12181 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    12241 cccataaact ctgcgcccgc tgggaagaaa ttgcctgccg ggcgcaaagt gacaaaacgc
    12301 gagtgtgcct gctgcgtacc ggtgtagtgc tggcaccgga tggcggtatt ctcggtaaaa
    12361 tgctgccgcc gtttcgtctt ggcctgggcg ggccgattgg ctctggtcgg cagtatctgg
    12421 cctggattca tattgatgat atggtcaacg gtattctctg gctgctggat aacgacctgc
    12481 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    12541 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    12601 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    12661 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    12721 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    12781 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    12841 aacctctatc cagcatctcc tgagcaatac ccagggcttc ttcgtgtttg ccctgcattg
    12901 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    12961 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    13021 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    13081 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    13141 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    13201 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    13261 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    13321 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    13381 tgcgaaaagc cattaattct tccggcttac tttggtgctc tatcactaca taaatataac
    13441 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtcgt
    13501 catcaataaa actgtttggt tccagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    13561 gcgcgggaag atgaatatca ataaaatccc gcgcggtgtc tggatggcgt caaaaagatt
    13621 taaataccgc atcatgcggc gtggaagttg tcgatattgt catggcgttc cgtcacccct
    13681 caaatttaag atgacgcgac aataaccacg attaacaccc aatgcagcag gcagttttta
    13741 tctttggaca gcgaattcag aagtattttt agcggactgc aacttttaca tttcagcgga
    13801 agctgactcg caaattcccc atgaaaatac tccggcagcg ttagccaccg gagaggaaat
    13861 tatttcagcg atcccttaag gaactgttgc agacgagggc tttgcgggtt gccaaataac
    13921 tgctccggcg cgccctcttc ttctattttc ccctgatgga ggaaaatgac atgagtagaa
    13981 acatggcgag caaagcccat ttcgtgagtc actaccacca tggttttccc ctcttctgcc
    14041 agttgctgca taatacgcaa cacttcgcct accagttcag gatcgagcgc cgaggtaggt
    14101 tcatcaaaca gtaaaacttc cggttccatc gccagcgccc gcgcgataga aacacgctgt
    14161 tgctgaccgc cggaaagatg caccggatat ttcccctgcg cacgttcgtc tatcccgact
    14221 tttgccagat acttgaccgc ccgctcgcgc gcttcctgct tgctcaggcc caacacctga
    14281 atcggcgctt ccatgacgtt ttccagcacc gtcatatggc tccagagatt gaagtgctgg
    14341 aataccatcg tcaggcgtgt gcgcagtaag cgcagttgat ttttatcggc gactttgagt
    14401 tgaccgtctt tgtcgcgcac cagattgatc gtctggccat tgaccacgat cgacccttca
    14461 ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac ttttccccga tcccgacgat
    14521 ccgatgatgc ttattacatc tccggcattc gcttgcagtg agaccccttt cagcacttca
    14581 tgttcgccgt agcgtttgtg caaatcgata acgtttaatt tattctcgga catcgtattc
    14641 tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt ttttccgctc tgcgaaagag
    14701 gctgatcagg acataagaga tgattaaata gagcaccgcg gcaatgccga aggcggtaaa
    14761 aggttgatac gtggcggcgt taatatcgcg ggctattttc agcagatccg gcacagtggc
    14821 cgtaaacgcc agcgcagtag agtgcagcat caggatcact tcgttgctgt atgccggtaa
    14881 cgcaatacgc agcgcagaag gcaaaataat gcaacgatac attttaaaag tcgagaagcc
    14941 ataggctctg gcggcttcaa tttccccatg cggaaccgaa cggattgccc cagcaaaaat
    15001 ctcggtagtg taagcgcagg tgttaagcgt cagcgccagc acggtacagt tcaggccact
    15061 gcggaagaaa gcgttaagga attcggttcc cttaacaatc tcaagcgtgt acatgccgga
    15121 atagaacacc agcaactgaa catacagcgg cgtaccgcga aaaatatagg taaataacca
    15181 gattggaaac tggatgtatt tattactgga gacacgacca atcgccagaa acagcgccag
    15241 gactccgcct atcactaccg acaaaataag cagccacaga gtgatcgcca caccagtaaa
    15301 gcgataaccg tcggtccaca gcagcggttt ccagtattca tgtaagattt cgatcacagg
    15361 tcagccctct tcacacccac ggagtagcgg cgctcaagga acagcagcac accattggaa
    15421 acagtggtga aaaccaggta aatcacgcca cagacgatgg cgaaatagaa cggttcccag
    15481 gtacttttgc ctgccagttg ggtggctttg accacatctt ccaggccgag taacgaaacc
    15541 agtgcggtag atttgaggat cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc
    15601 atcatcgccg gaaacatgat ccgccgaaac acttgcccac gagtaaaacc gaacgccgtc
    15661 gccgcctcta tatgcccttt cggcactgcc ataaacgcgc cacggaacgt ttcggtaaaa
    15721 taagcaccgt agataaaacc gagagtaata ataccagcga ccatcggatc gatatcaatc
    15781 tgcccgacgc ccatcgcctc cgtcaccgtg tttagtgcaa tctgcaaacc gtagaaaatc
    15841 agcagcatca acactaagtc cggcacgcca cgaatcagcg tggtgtaccc ttcgaaaatc
    15901 agccccgaaa gccgattttg cgagagctta ccgccagcgc caattaaacc gatgattaca
    15961 gcgagcacta cagaactgat agccagctcc agcgtgacga gcgcaccctg taaaataaca
    16021 cctgaaaacc catacaacat gctgcctgtc ctgtcatgtg tggtgaatta ccgcctcttg
    16081 tctccctccg gttacccgaa gggagcgggc tttctttcac cgatggcgat taaccgccat
    16141 aaacatcaaa atcgaagtac tttttcgcta atttctcgta agtaccgtca gcgcgcattt
    16201 cggcaaaggc tttgttcagt gcctcgcgca gttcgttatc ttctttacgc aggcccatgc
    16261 cggtccctac accaaacagt ttttcatctt taacagacgg gccaccgaat ttgtaatctt
    16321 taccgacggg ttgtttgagg aaaccttcac tggcagcgac ctcatcctgg aacgcggcat
    16381 caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg cccctgatac gagacgattt
    16441 caatgccttt tggtgcccag tgttcgttac cgaacgtctc ctgggtggtg ccctgcaata
    16501 cgcctacccg cttgcctttc agcgactcga ctgtcggctg aatgtcggaa tttttcgcca
    16561 ccaccagacg agaatcggca gcgtacagtt tgtcggtgaa ggcaatttct tgctgacgtt
    16621 tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt cttcgctttt aaggacggga
    16681 ttaacgcatc cagcggattt tcgacaaacg tacattgcgt attgatgcgt ttgcataatt
    16741 cctttgccag atcgatatcg aagccaacca gttcgccttg tgaatttttt gattcaaatg
    16801 gcgcataggt cgggtcggta ccgatgcgga tgttttgcgg aatcgcagca agtgccgcag
    16861 ttgcgctgga gaaggccaga accagagaga gcgatagcac cagttttttc ataatgtcct
    16921 caactgacag tctttttata gggaatattt acaggtttgt tacacttatc gtgccataaa
    16981 attgaccatc aaggcaaaat atcccgcccc gttagacgta ttatttttca ttaaaaatgc
    17041 ttaagtatgc atttcaatgc accacggtag tgcaacaaac ctggcgtgca ccatgatgat
    17101 cattccatca ggtacagctt cccagcgacg tatcagtcac cgtagacatt aaagtcgaaa
    17161 tactttttcg ccatcttgtc gtaggtgccg tcctgacgca gctcgccaag cgccttattg
    17221 aaggcagccg tcagttcagc atcatcttta cgtagcccta caccggtgcc atcaccgaag
    17281 tattttttgt cttttactga tgagccagca aaggcgaaat ctttaccagc aggttgcttg
    17341 aggaatcctt cgctggcagc aacttcatct tgtaacgcag catccagacg tcctgcagcc
    17401 agatcggaat agaccaaatc ctggttggca taggccacca catccacgcc tttgctacgc
    17461 caggtctcgt tagcgtaagc ttcctgggtt gatccctgca gcacaccaac atgtttacct
    17521 ttcagtgaat ccagcgttgg ctgaatcggt gaacctttgg cagcaatcaa acgagaatct
    17581 gcggcgtaca gcttgtcgga gaaggcaatc tcctgctgac gtttatcggt aatggaaagc
    17641 gacgaaataa tggcgtcgat ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca
    17701 ctggcaaccc aggtacattt cacctgcatc cgtttgcaca tctcgttacc gagatcgata
    17761 tcaaagccaa caaaatcacc tttagcatct ttcgatgaga acggtgcgta ggtggtatcg
    17821 gttccgatac gtaccgtctc cggtagcgcc gcatagctgg aagccgctgt ggagagaccg
    17881 actaacaaag acagagcgag aatcgacttc ttcatacata accctcaagt gaaaatggct
    17941 tttttatgtt gtgtattgtg ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat
    18001 cttgacgaca acagtaacat tcaacgttaa atatgttaat aagacgttgc attattgtcc
    18061 tgaagttgaa gatagcaggt atagcggttg aatcgcagcg tttcgattga atggcagaaa
    18121 caaaatgtcg aggatttgat cgcggttgca aaattgccct gaaacagggc aacagcggag
    18181 ttatgcgccc tgccagcggg caaagagatc ttcaggaagg gttatcgcaa actggtcaag
    18241 aacacgatta accgtctgat ttatcacatc atcaagggat tgcgggcgat gataaaacgc
    18301 cggaacggga ggcataatca ccgcaccgat ttctgccgcc tgagtcatta aacgcagatg
    18361 gcctaagtgc aatggtgttt cacgcacgca gagcaccaac gggcgacgct ctttcagcac
    18421 cacatctgcc gcacgggtca gtaagccatc agtatagcta tggacaatgc cggaaagggt
    18481 tttgattgaa cagggtaaaa tcaccatccc cagcgtctgg aaagaaccgg aagagatgct
    18541 ggcggcaata tcgcgcgcat cgtgcgtgac atcggctaat gcctgcactt cgcgcagaga
    18601 aaaatccgtt tcgagggata aggtctggcg cgctgcctgg ctcatcacca gatgcgtttc
    18661 gatatctgtg acatcgcgca gaacctgtaa taagcgcacg ccataaatcg cgccgctggc
    18721 accgctgatg cctacaatga gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag
    18781 actttgcagg atttcggcgg gagttgcaag tcagggtgcc agaccggcac cctcagcgaa
    18841 ggcatcatcc ttcgttatgc atttcgagat tttccacttc gttctgacgt tgcactgctt
    18901 tggcgtcatc attacgtaac gtatcgagga aatcgaggta gccctgatca acgtctttgg
    18961 tgacgtagac gccgttgaat accgagcatt caaactgctg aatatccggg ttttccgcgc
    19021 ggacggcttc gatcagatcg ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct
    19081 ggcgaatttc atccacttca cgaccgtgag cgattagctc cgtggcgctc ggcatatcaa
    19141 taccataaac gttcgggaag cgaatttccg gtgccgcaga agcgaggtac actttcttcg
    19201 ctccggcttc gcgtgccatc tcgataatct gctcagaagt ggtgccacgg acgatggagt
    19261 cgtcgaccag caggacgttt ttatcgcgga actcggcgcg gttggcattc agtttacggc
    19321 gcacggactt acgacgcagc tgctggcccg gcatgataaa ggtgcggcca acatagcggt
    19381 ttttaacgaa gccctggcgg tacggtttgc ccagaatacg agcaatttcc agcgcgatat
    19441 cacacgaggt ttctgggatc gggatcacca cgtcgatatc cagatcttcc cattcgcggg
    19501 caattttctc gcccagtttc gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt
    19561 cgataaacga gtccgggcgg gcaaagtata catactcaaa caggcacgga ttgctgaccg
    19621 gattgtcagc acattgacgg gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc
    19681 ccggcgcgac gtcacgcagg aaatcaaagc ccagcgtatc gagcgctacg ctttcggaag
    19741 cgaccatata ttctgtacgg ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa
    19801 tcccgtttgg atcgcggaaa gcaaccatac cgtggccgat aatcatcgcc acacaggcat
    19861 acgcgccgcg gattaagcgg tttgtggcag caatggcagc gaaaatattg tcggcttcca
    19921 gcgggtagtg gcggaagttg tccagctcgc tggcgaagat attaagcaga atttccgagt
    19981 cggaagtggt gttgatgtgg cggcgttttt cttcaaacag ttttttacgc aactcgtgag
    20041 cgttggtcag attgccgttg tgggcaagcg taatgccata cggggagtta acgtaaaacg
    20101 gctgcgcttc agaggcgctg gagctgccag ccgtggggta acgcacatga ccaatgccca
    20161 tattgccctg caaacgctgc atatggcgag cttcaaatac atcgctcacc agcccgttcg
    20221 ctttacgcaa acggaagcag ttattggcat ctatggtgat gatgccggcg gcatcctgac
    20281 cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg gttaaccggc ataacaccgg
    20341 cgataccgac aataccgcac atacgtcttt ttcctcgtta agccacatct cagagcactt
    20401 acgctctggg caagaaactt gacgagcttt gcagataatc aaaaaaccat ctgatgataa
    20461 aactgaattg cgggatcagc tgtgatttgc tccagtcttc gcttttcgac accccggtaa
    20521 aggagtcgag aaagaagaga atggcggcaa caatcaacac accgcgcaac gcaccgaaac
    20581 agacgcccag cacccgatcg gtgcctgaca accccgtttt ctccaccaac tggcctatca
    20641 cgaagttcac gatagcacca acgatcaggg tagcgataaa cagtaccgcg atggcaatcc
    20701 catttcgaac cagttcgtct tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat
    20761 gactggcaac aaagaaagca caaccccatg tcaccagcga taacgcttca cgaacaaagc
    20821 cgcggatcag gctaaccaga gaggaaaaag caatcaccgc gattatggcg taatcaatcc
    20881 agaccatatg tgtcccacga ttttacgccc tgtcatcctg ttcggggcgc attctaacag
    20941 aaaaagaaaa cgtttgcgta gggatttcct tcccgcgcat caataaaaat ggcgctgaaa
    21001 aaatattcaa cgccatcgac tttttatgcc tttgcggcat cgggcaatgc gtgtcggatg
    21061 cggcgtaaac gccttatccg acctacggtt ctacccctgc gtaggcctga taagacgcgc
    21121 cagcgtcgca tcaggcaaga ccgtattaat tcggcgtata gcccattacc acgccactta
    21181 agccagaaag ttgcttcaac tcacccagcg aacctttcag cttatctttc gaggcatccg
    21241 gcccaaccag aatacgggta attttaccct gcactggcgt ggatggcgac gtataaaccc
    21301 gataaccggc accgcgcagc ttaccgacaa tctcattcac tttatcggca tttttcagcg
    21361 cacccagttg cacaacatag gctttacccg tcggtgcagc tttttcttcc acgaccggct
    21421 tcggttctgg cttcggcgca ggtggtgctt caacctttgg tttaggcggc tccaccggtt
    21481 tcggcttcgg tggggcaacc ggtgcaggtt ccggttcaaa ctcggtgtta ttggctgcaa
    21541 tagtggccgg atcgagcgac ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc
    21601 cttccggcgg ctgcgtcggt aacgcctggg tggcggctgg catcatatca ggctcatcac
    21661 gatcgcccgc tttcggcacc agcgggatag ccgcgaactc atcctgataa tgttttttct
    21721 gcccgtccag cagccctgga agtacaatca cccccagcgc caccagcacg atcgtgccca
    21781 ctaaccgatt ctgaaactta cttgccaccg cttctcctcg cgtcaatcac ttccatgaca
    21841 tgtgcgaccg tgtggaaaga accacacacc agcacggtgt cttccgcttt agcgtccgcc
    21901 attgcggcat cccatgcctg cgcaacgcta tcaaatgatt tgccgttacc caaatgctca
    21961 agcagttgtt ctgccgtggc accgcgcggc ccttccagtg gcgcacaata ccagtcatca
    22021 accacgcttt tcaaccaggc cagagttccg gcaatatctt tatcatgtag cataccgata
    22081 accgccagca cgcgcccgtt tttcggtagc gctttcatac gcccggtgag atattccgcc
    22141 gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg actcgctcac aatctggaaa
    22201 cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg cattttcact gacttccagc
    22261 ccgctggcac gcagtgccgc cagcgccgtt gcggcattcg gttgcgggac aagcggcaac
    22321 ggtaggtttt ccagcgtgcc gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa
    22381 tagttccact caacgccccg acgttgtaac agtgcacctt tttcctgcgc cacatcagca
    22441 atggtagaag gcatttccgg ctcaccgaca attgccggtt tttcgctgcg aaagatgcct
    22501 gctttctcgc gaccaatact ttcgcgatct ggccccagcc agtcggtatg atccagcgca
    22561 atactggtta ctaccgcgac atcggcgtcg acaatattgg ttgcgtccag acgaccgccc
    22621 agccctactt ccagaatcac cacgtcaagt tgtgcctgtt tgaacagcca caacgccgac
    22681 agcgtaccgt actcgaaata ggtcagggaa atatcaccgc gtgccgattc aatctccgca
    22741 aaagaggcgg tgtgggccga ttccggcaat tcctgaccct gcacacgtac gcgctcggta
    22801 taacgcacca gatgcggcga actgtagacg cccactttgt accctgccgc catcagaatc
    22861 gactccagcg tacggcaggt ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat
    22921 ggcgctggtt tcaggacgcc aagacgagcc gcgacctggc tcacgcgctc aaggccgaga
    22981 tcgatagttt tactgtgcag gttttccaga taagaaagcc acgaagccag aggcgacgcg
    23041 gcttgaggag tgcgtttgat aatcatggta tccgctgatt cgttacggtg agaatagcaa
    23101 aagggcagag ccagtggccc tgcccttatc agttatcagg cctcaggttc ctgatccggt
    23161 accgggggta ccactacgcc ttcacgcggc gcttcaggat tcggcgctgg cagattcatc
    23221 aacttcgcca gaatgctcgc cagtttcagg cgcatttccg gacgacggac gatcatgtcg
    23281 atcgcgcctt tctcgatcag gaattcactg cgctggaatc ctggcggcag tttttcgcga
    23341 acggtctgtt cgataacacg cggaccggca aagccgatta acgctttagg ttcagcgatg
    23401 ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc ccatcgtcgg gtcggtcagc
    23461 acggagatgt acggcaagcc gcgctcctgc atttttgcca gtgccgcaga ggttttcgcc
    23521 atctgcatca gcgacatcag tgcttcctgc atacgtgcgc caccagaggc ggagaagcag
    23581 atcagcgggc agttatcttc cagcgcctgc tcaacggcac gcacgaaacg tgcacccaca
    23641 acagacccca ttgaaccgcc cataaaggcg aactcgaatg ccgcagcgac aaccggcatt
    23701 ccatacagag tgcctttcat caccaccagc gcatcttttt cgccggtttc tttctgcgca
    23761 gatgccagac ggtctttata cttcttggag tcacgaaact tcagcacatc tttcggctca
    23821 agctcgctac ccagctccac aaggcttcct tcatctaaca ggctatgcag gcgattacgc
    23881 gctgtcatac gcatgtgatg gtcacacttc ggacagacct caagattacg ttccagctca
    23941 gcgcggtata aaacctgacc gcagctatca cacttagtcc acaccccttc aggaatgctc
    24001 gccttgcggg tgggagtaat gttgctttta attcgttcaa tccagctcat tagggacctt
    24061 tctgtctgaa cctggttcga tgccagtttt atctttgggg acgcataatg ccatttttgc
    24121 ccccaacaga ccatgaatgt tgcacattaa aacataacag cccgagactt tggataaaaa
    24181 agtggtcgaa ccgcagagtt actttttatt ttgcggcgcg tgccgcagcg cgtttgtgac
    24241 gaataaattc gaaaacccct ggcaatattg aaacaaaaat aatgccaaca atcatcagct
    24301 taaggttgct ctgaatgaag gggagcgtgc cgaagaaata acccgcgtag gtaaaaatca
    24361 gtacccacaa cagtgcgccg atcacgttat aagcggcgaa atgacggtac gacatgtggc
    24421 ccattcccgc aacaaacggc gcgaacgttc tgacgatcgg tacaaaacgg gcgagaataa
    24481 tcgttttgcc gccatgtctc tcgtaaaact gatgagtctt atcaagataa ctgcgacgaa
    24541 agattttcga atccgggtta ctgaacagtt tgtcaccaaa taaccgtcca atggtatagt
    24601 tcactgcatc accgacaata gcggcaatca acataagcac caccatcata tggacattga
    24661 gatcgttagt ttccagcgac gccaatgcac cagcaacaaa cagcagcgaa tcaccaggca
    24721 ggaatggcgt taccaccagc cccgtttcgc agaacagaat caaaaacaga atggcataaa
    24781 cccagacgcc gtactccgcg accagctctg ccagatgcac gtcaatgtgc aggataaaat
    24841 cgatgaggaa ataaatcagg tccatatttg cctatgcctt gtactcgtca tctttcaggc
    24901 tgtaactacg ttggctacag cctgaaatgt tccgagcatt attctcgtta gtccgccaga
    24961 aatagcgggc ccattggcgg ttttggaaga tcataccggt cagggtaatc caccgcgacc
    25021 agatacagcc cttccgcttt tgccgttgct gccgccagcg ttctgtcctt tgccgccagc
    25081 aactctgcta tccagctctc cggttggttg tgggcaccga cttccatcag gctgccgaca
    25141 atattcctga ccatatgatg tacaaaggca ttcgctttga tatctaccac cacataagga
    25201 ccgtgacgcg tgacattaat atgcataacg ttgcgccacg gggttcggga ctggcactgc
    25261 accgcacgga acgaggtgaa atcattctcg cccagcaagc attgcgcagc ccgatgcatc
    25321 cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt tactcagtac cgccgggcgc
    25381 agccgatgat tgtagatgat gtagcgataa cggcgagccg tggcgctaaa tcgggcatga
    25441 aaatcatcag gtacagcttt aacccaacgc acggcgatgt caccaggtaa attcgcattt
    25501 acgcccaacg tccacgcagc gtctttgcgc tgcgcggtag tttcgaaatg caccacctgc
    25561 ccggtaccgt gtacaccggc gtcagtacgc ccggcgcaga agacggtgat cggttcgttc
    25621 gccacctggg agagcgcctt ttccagtttc tcctgcacac tgcggacttc attctgccgt
    25681 tgccagccgt aatacctact accgtcgtac tcaatgccca gcgcaatttt ataaactggc
    25741 ggttgttgct ggtcggacat tagtacagat actcctgcac cagtttctcg gcgattttta
    25801 ctgccatcag cgcgccgcca aagcgaacgt tatcggccac cgaccagaac tggacttgct
    25861 ccggcatacc gtagtcatta cgcacgcagc caacagaaag atgcggcgta cccgaagcat
    25921 cacctacctg agttgggaat tcgttctctt cagagagcac aatatcttcg ccttgaacaa
    25981 acgcatcacg cgcttcttct gctgccagtg gacgcagagc ttcaaagttg accatctggg
    26041 catgaccgta gaataccggt gcctggacga cgctagccga aatcatcagc ccttcgtcct
    26101 gcaggatttt gcgtacttcg tcaacgatac gacgttcttc acgcacgcta ccttcgctat
    26161 ccggcagtaa cggcagcatg ttgaacgcca gctgacgccc gaagaaatct tcttcgtcaa
    26221 tcggaatgcc gttgagcaat ttcgcactct gccccgctaa cgcatcgacc gcttttttgc
    26281 cctgggcgga ggctgaaatc aggctggtaa cgctgatacg tgataaaccg ccctgatcga
    26341 ttaacggttt cagtgccgcc agcagctggc tggtcagact gtctggtacg gcgatgacat
    26401 tccggttccg gtaatctgtc agtacaaacg ggtttacttc cggcaccacc agcggtacgt
    26461 cgggttcgag agcaaacaat ccactgctgt cgatcaccag gcaacctgag ttggtcgctt
    26521 cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg
    26581 tccagtcgaa ttcagcggca tcctgcacgg tgattgtctt accaccaaag cgcagttgtt
    26641 cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc aaccgggaac tgacgttcag
    26701 ccagcgtttc aagcagggct tcgcccacag cgccagttgc gcccaggacg gcaatgttcc
    26761 agccttcaga catggtggtt tactccagaa atagcaaagc tccctgccaa tgttacagca
    26821 gagagcatga agaagagatt aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca
    26881 cagcaatgat gccgcactgg cgtcatcaca aattacatac agagatgacc attcacggcg
    26941 ctcaagatag tttttgcgca gtttatcgaa ctcacccggt atcccggcga ctttacgcag
    27001 cggtgcgtca tcgcggcgca catcatacac caaatgcacc agccttttca gcgtcggttg
    27061 atcgagcggg ccatgcagcg taatgcgacc aaactctggc gcgggcagta atgtatccag
    27121 cgcaacgtgc tgttcatgcc caataaactt gctataggct tcaaacactt gcgtcgtgcc
    27181 acgcgcttta ccctccaggg tatagcctgc gatatgcggc gtgccgatat ccacttttgt
    27241 cagcaattcc acattaagtt ccggctcgcc ttcccagaca tccagcacca cgcttaactt
    27301 ctggccttca ttcaggcagg tcagcagcgc ggtattatcg acgactgcgc cacggcaggc
    27361 gttaatcaga atcgctccgg gcttcaggct acggatcagt ttttcatccg ccagatgtag
    27421 cgttttgtac ggaccatctt taaagagtgg cgtatggaaa gtcagaatat ccgcgcgctg
    27481 gactaactca tccagcgagc ggaaatcacc ctcatcccca cggtcggcgc gaggcggatc
    27541 gcaaagtaag gttttaatcc ctaacgcttc cagtcgcgcc tgcaatcgac gtccaacgtt
    27601 accaacgccc acgatcccca ccgtacggtc gtacagtgaa aatccatcgc gttcggcaag
    27661 catcagcagg gaggagaaaa catattccac caccgcaatc gcattacagc caggtgcagc
    27721 ggaaaaacca attcccgcct gcttcaacca tgcttcatcg acatggtcgg tccccgcagt
    27781 ggcagtgcca acaaatttaa tgggttttcc tgccagcaaa gattcattca ctttcgtgac
    27841 cgaacgcacc atcagcgcat ccgcgtctgc cagttgagcg acggggattg gacgcccggg
    27901 aaccgcggtc acctcaccca aacggctaaa taagtcgcgg gcataaggca tattttcatc
    27961 aacaaggatt ttcacgtttg tgttacctgt atgagacgag agttaaccgg acaagtgtgc
    28021 cataatctcg cggccaggca tacttgcgaa gatttcaggt ataaggatac gtaatgatac
    28081 aacctatttc cggccctcct cctgggcaac caccaggtca gggagataat ctgccgtctg
    28141 gcacgggcaa tcagccttta tccagtcagc aacgtacttc gctggaaagc ttaatgacga
    28201 aagtgacctc actgacgcaa cagcaaagag cagaactgtg ggcgggtatc aggcacgata
    28261 ttggtctgtc gggagattca ccgctgcttt cgcgtcactt ccctgccgct gagcataatc
    28321 tggcgcaacg tctgctggcc gcgcaaaaaa gccattctgc ccgccagctt ttagcgcaat
    28381 taggggagta tttacgtctg gggaataatc gtcaggcggt cacggattat atccgtcata
    28441 actttggtca gacgccgctg aatcagctct caccggagca attaaaaacc attctcaccc
    28501 tgttgcagga agggaagatg gttattccgc aaccacagca gcgcgaggcg accgaccgtc
    28561 ctttattacc ggcggagcac aatgcgctaa aacagctggt gaccaaactt gcggcggcaa
    28621 cgggggaacc cagcaaacag atctggcaat cgatgctgga actttccggg gtgaaagatg
    28681 gcgagttaat tccagcgaaa ctgtttaacc atctggtgac ctggctacag gcgcgtcaga
    28741 cgctaagcca gcaaaatacg ccgacgctgg aatcactaca gatgacgcta aaacaacctt
    28801 tagatgccag tgaactggcg gcgttatcgg catatatcca gcaaaaatat ggtctttctg
    28861 cgcaatcatc gctttcttct gcccaggccg aggatattct taatcagctt tatcaacggc
    28921 gggttaaagg gattgatccg cgtgttatgc aaccgctgct taatcctttt ccaccgatga
    28981 tggacacgtt gcaaaatatg gcaacgcgtc ccgcgctgtg gatactgtta gtcgcgatta
    29041 tcctgatgct ggtctggctg gttcgttagc cccgacgaaa tgacagtatc gtgacaataa
    29101 ttcccagcac cgcagagatc gccccggcaa gaaataccga agagtaacca aacgtggtcg
    29161 ccagcattcc cgccagcggc ccggagacgc cgagggcgat atcctgaaac gcggcgtaac
    29221 cgcccagtgc ggtgccgcga acttgtgagg ggacgcgttt aaccacctcc acgcccagcg
    29281 caggaaagat aagcgaacat ccggctccgg ttaacgccgc gcccgctaat gcgacccacg
    29341 cacctggggc ttgccagagc agcaacaagc ccaccgtttc tacaagcaga gagacaatcg
    29401 ccactttcac gccgccaaaa cggtccggca tccagccaaa catgacgcgc atcacgacaa
    29461 atgcgccgcc aaacgcggta agagtaaagc ccgccatcgc ccatcctttg ctggcaaagt
    29521 agagcgaaac gaaagtcccg ataaccgcaa aaccaacgcc ttgtagtgcc agccctaacc
    29581 ctggtttcca gataaacccg acaacgctcc acagcgatgg acgttctccc gccagggccg
    29641 gtactttgcg cactgtgccg ttacaggccc acgccagtaa gggtaatacc attgtggtga
    29701 tcgccagtgc ggcaaaaccg taatggctat gaatcaacag gccaagcgga gcaccaacag
    29761 cgagggcacc gtaaatcgcc attccattcc atgacatcac tttgccagag tgttttggcc
    29821 ctacgatgcc taacccccag gtcagagcgc ctgtcagtaa ctggctttca ccaaacccaa
    29881 gaatcaaacg cccgatgacc aacagggcaa atttgaacgg tgcggagaca ggcaaaatcg
    29941 ccgccagcag caacgcgccg ccagccagac cacaagctaa catcccctga agcgccgaac
    30001 gttttgcacc atattgatcg gccagtcgcc cggcatagcc acgcgtcagc accgtagcca
    30061 gaaactgaat cccgacggca atcccgacca tggtattgcc atagcccaga tcatgatgaa
    30121 caaacagcgg gataaccggc aacggcagcc ctacggtcat gtaggtgaga aaaaccgcaa
    30181 aagcgatgcg gaagagcgaa aaattggcag aagatcgtgt ttcggtttgg cttacagcag
    30241 tcatgcatta ctccagaatg cagcgcaagg cgaggagtat ccccgtctca tctctctggt
    30301 ttcagggtta cggtgcgttg gcaggattta acgcgtacgt cttttcagaa ggaaatcgac
    30361 aaagcgggaa gtttgcctgg aacaggcggc gattgtcaat gatgtgaaaa agggaaccat
    30421 caggttccct tttgcgttag tgccggaggc cgcattagcg cgtaacgtcg gatgcgacgc
    30481 tggcgcgtct tatccgacct actccgacta ctccgactac tccggcctac tgcgaattaa
    30541 tctttcagct tgcgcattac cagcgtggcg ttggtgccgc cgaagccgaa gctgttagac
    30601 ataacggtgg tcagttcgcg atcggtcgtt tcggtcacga tgttcagacc tgcagcctgc
    30661 tcgtccagct cttcaatgtt gatgctcggg gcgataaagc cgtgttccag catcagcaga
    30721 gagtagatag cttcctgtac gccagcagcg cccagagagt gaccggtcat ggctttggtt
    30781 gcagaaatcg ccgggctctt atcgccgaac acttcacgga tagctgccag ttctttcacg
    30841 tcgccaaccg gagtcgaagt accatgagag ttcaggtaat cgattggggt atcaacgccg
    30901 tgcatcgcca tcttcatgca gcgtactgcg ccttcgccag acggagcaac catgtctgca
    30961 ccatcagagg ttgcgccgta gccaacgatt tcagcataga tgtgagcacc acgcgccagc
    31021 gcgtgttcca gctcttcaac cactaccata ccgccgccgc cagcgataac gaaaccgtca
    31081 cggtgagcgt cgtaagtacg ggaggctttt tccggggtgt cgttgtattt agtagacagc
    31141 gcacccatcg cgtcgaattc gcaggccatt tcccagcaca gctcttcgcc gccgccagca
    31201 aacacgatgt cctgtttgcc cagttggatc tgctctactg cgttaccgat acagtgtgcg
    31261 gaagtcgcac acgcggagct gatggagtag ttaacgccat gaattttaaa cggcgtggcg
    31321 aggcaggcag aaacgccgga tgccatcgct ttggtgacca catacgggcc aaccgctttc
    31381 aggccgcgcg ggccgcgcat tgcgtcagcg ccgaacacct ggaaacgcgg ggaaccgccg
    31441 ccagaacctg caatcaggcc aacgcgcggg ttattctggt aagcttccgg agaaaggccc
    31501 gcatctgcga ttgcctgctc catagaaagg aatgcataaa tggatgcgtc gctcataaag
    31561 cgcacaactt tgcggtcaat gaggccagtg gtatccagtt ttacgttgcc ccagacgtgg
    31621 ctacgcatgc cggaatcctt cagctcctga gagaaagtga tccctgaacg tccttcacgc
    31681 agagatgcca ggacttcctg ctggttatta ccgatgctgg aaacaatgcc caggccagta
    31741 atcactgcac gtttcattca atacctctgt aagtcgcaca tagagtaagt ttcgaatgca
    31801 caatagcgta cacttgtacg ccgaacaagt ccgatcagcc atttaataga gaaatttgcg
    31861 cagccttaca cacatcgcta agatcgagcc accgcctgta agacgagtaa cttacgtgaa
    31921 acactactcc atacaacctg ccaacctcga atttaatgct gagggtacac ctgtttcccg
    31981 agattttgac gatgtctatt tttccaacga taacgggctg gaagagacgc gttatgtttt
    32041 tctgggaggt aaccaattag aggtacgctt tcctgagcat ccacatcctc tgtttgtggt
    32101 agcagagagc ggcttcggca ccggattaaa cttcctgacg ctatggcagg catttgatca
    32161 gtttcgcgaa gcgcatccgc aagcgcgatt acaacgctta catttcatta gttttgagaa
    32221 atttcccctc acccgtgcgg atttagccat agcgcatcaa cactggccgg aactggctcc
    32281 gtgggcagaa caacttcagg cgcagtggcc aatgcccttg cccggttgcc atcgtttatt
    32341 actcgatgaa ggccgcgtga cgctggattt atggtttggc gatattaatg aactgaccag
    32401 ccaactggac gattcgctaa atcaaaaagt agatgcctgg tttctggacg gctttgcgcc
    32461 agcgaaaaac ccggatatgt ggacgcaaaa tctgtttaac gccatggcaa ggttggcgcg
    32521 tccaggcggc acgctggcga catttacgtc tgccggtttt gtccgccgcg gtttgcagga
    32581 cgccggattc acgatgcaaa aacgtaaggg ctttgggcgc aaacgggaaa tgctttgtgg
    32641 ggtgatggaa cagacattac cactcccctg ctccacgccg tggtttaatc gtacgggcag
    32701 cagcaaacgg gaagtagcga ttatcggcgg tggtattgcc agcgcgttgt tgtcgctggc
    32761 gttattacgg cgcggctggc aagtaacgct ttattgcgcg gatgaggccc ccgcgctggg
    32821 tgcttccggc aatcgccagg gggcgctgta tccgttatta agcaaacacg atgaggcgct
    32881 aaaccgcttt ttctctaatg cgtttacttt tgctcgtcgg ttttacgacc aattacccgt
    32941 taaatttgat catgactggt gcggcgtcac gcagttaggc tgggatgaga aaagccagca
    33001 taaaatcgca cagatgttgt caatggattt acccgcagaa ctggctgtag ccgttgaggc
    33061 aaatgcggtt gaacaaatta cgggcgttgc gacaaattgc agcggcatta cttatccgca
    33121 aggtggttgg ctgtgcccag cagaactgac ccgtaatgtg ctggaactgg cgcaacagca
    33181 gggtttgcag atttattatc aatatcagtt acagaattta tcccgtaagg atgactgttg
    33241 gttgttgaat tttgcaggag atcagcaagc aacacatagc gtagtggtac tggcgaacgg
    33301 gcatcaaatc agccgtttca gccaaacgtc gactctcccg gtgtattcgg ttgccgggca
    33361 ggtcagccat attccgacaa cgccggaact ggcagagctg aagcaggtgc tgtgctatga
    33421 cggttatctc acgccacaaa atccggcgaa tcaacatcat tgtattggtg ccagttatca
    33481 tcgcggcagc gaagatacgg cgtacagtga ggacgatcag cagcagaatc gccagcggtt
    33541 gattgattgt ttcccgcagg cacagtgggc aaaagaggtt gatgtcagtg ataaagaggc
    33601 gcgctgcggt gtgcgttgtg ccacccgcga tcatctgcca atggtaggca atgttcccga
    33661 ttatgaggca acactcgtgg aatatgcgtc gttggcggag cagaaagatg aggcggtaag
    33721 cgcgccggtt tttgacgatc tctttatgtt tgcggcttta ggttctcgcg gtttgtgttc
    33781 tgccccgctg tgtgccgaga ttctggcggc gcagatgagc gacgaaccga ttccgatgga
    33841 tgccagtacg ctgacggcgt taaacccgaa tcggttatgg gtgcggaaat tgttgaaggg
    33901 taaagcggtt aaggcggggt aatctgctct cgcagtgttt gccggatgcg gcgtgaacgc
    33961 cttatccgac ctacgtgtga cgtgtaggcc tgataagacg cgataagcgt cgcatcaggc
    34021 atggtgctcc agacgccgca acttactgct gtgacgcctg ctgaaacagg ttttcccaca
    34081 tatcggtcac cagcgcctgg tcacgcggcg acaattcacc ggcaccaatg gctttttcca
    34141 gactctggct aacggtcgta tgcaccgcct gagcggagtg gtcatcacca ctttccagtt
    34201 ctgcgatggc taatgtcagg tggccacgca aatacccact ggcaaacaac tcatcatcac
    34261 tggcatggtc aaccataccg tcgattaatg ccagaatgcg tgattcaaac tccgcgatca
    34321 tcttctttcc tcattgtaaa acgtggcacc ggcttcagtt gagcgcttcc ggccacggga
    34381 actgttccgc cgtaagttcc ggcgtgtgat aataattttg tagtgcttta atgaagcgtg
    34441 ccggacgctc gggaatgcca tttgtcagat aatccatcac ctgcgcatgt acccgccgtt
    34501 gaaacaccac acgatccggt tcgaagtcgc cttccagatt gtcgcaactg acgttaaacg
    34561 gatatcccgc cgccacacag aacaaccagt cgagtgcctg cggcttcact tcaacatctt
    34621 caaactggct ttgcgtttgg gcatcacgcc catccgggca ataccagtag ccgaaatcaa
    34681 ccagctcacg gcgcgctttt ccggcaatac accagtgcga aatctcatga attgcgctgg
    34741 cataaaagcc gtgggcaaag acaattcggt tatacggtac ttccgcatca gcaggaagat
    34801 agatcggttc gtcgtcgcct ttaatcagac gggtattaaa ttcatcggca aagcagctat
    34861 taaaaatttc aatcaactgc tcatagtggt gtgtactgtt cattagttca tccccaacca
    34921 gtggaggatc tcctgtccgt ggctatcgta aagtaatttg gcactcatca ccgccgagac
    34981 gataacaatc atcgggcgga tcagcttttg tcctttgctc aacaccagcc gtgaacccat
    35041 gcgcgcgccg aggaactgcc ccaccagcat gacaaagccc gtcgcccaaa tcactttgcc
    35101 gccgagaata aacagcagca gaccgccaat gtttgacgtt gcgttgagta atttggcgtg
    35161 agccgtggct ttggcgaggt taaatccgca cagcgtaacg aaggccagcg cgtaaaacga
    35221 cccggcagcc gggccaaaga atccatcata aaaaccgacg cagccaccgg ctattaacgc
    35281 gaagggtaaa ccgtacatcc ggcgctggcg gtcttcttca cctagctttg gcatcagcaa
    35341 gaaatagagg ccgatacaaa ttaccagaat gggcaaaatc tgccgtaaga catcagcctg
    35401 aacgtattgc accagcagtg cgccgctcat tgagccgaca aaggtcatgg cgatattgag
    35461 tttctgatcg cttaaactaa ccactttgcg gcgaataaag tagatagtag cggaaataga
    35521 gccgccgcag gcttgcagtt tattggttgc cagcgcatta gcgggagaca tccccgctgc
    35581 catcaatgcc ggaatggtga gtaacccacc gccaccggca atcgagtcga taaatcccgc
    35641 cagcatggcg acaaaaaaga gaactcccag caacagcggg gaaaccataa acaggctatt
    35701 aaacgtttcc attagatcac gtgctcatcc agtagcgcct ggcaggaagg cggcaacgga
    35761 ggcggtgtct tcttctcagg ctttgttgtt cccggttttg gaggttcaaa ccagctttgc
    35821 agttctgccc cgcaaccatc gcctgatggc ggtaaaggtt gatcttcaca ctccagacta
    35881 tcggcagggc aacgtaatcg tacatgcata tgcgcgcgat gctggaacca gggtcgcact
    35941 ttgcgcaacc agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgttt aatcgccgga
    36001 ttaacaaaaa tgcgcgtgac gtctttgtcc tgggcggcga gtttgatcaa gctgaaaatt
    36061 tctggcttcc acagcgtgga gacaacgtgt ttaccgtcgc gggaaactaa gtccagtgct
    36121 tgcgggcgca agagctgcgc ggaggtccag cgagttttcg gcagttgcag aaagatatcg
    36181 acatccagtc cggtctggtg gctggcatga ccgccgttga aacgcccacc agcgggcatc
    36241 cccatatcgc caatcagcac cgtacccatg cccagattgc tcacctggct actcagacgc
    36301 tggataaaca tcaccagatc cgggtgaccg aaatagcgac gctgatcggt acgcatgacc
    36361 tgataatgtt cggactgtat cggcaacgtg tcagcgccga caatacagcc attagaaaaa
    36421 ctgcctatcg attgtgcgct acccggcaca ggttgggtta ttttttgcca cggcgtcgct
    36481 gccaggctag cgctactggc aagcagagcc agcagcgcaa tcgcggtttt gttcattttt
    36541 taccagcgtg gaatatcagt cttcacatcg gcattttgcg cccgctgccg taacaggtga
    36601 tccattaaaa cgatcgccag catcgcttca gcgatcggca ctgcacggat cccgacacag
    36661 ggatcgtgac ggcctttggt gatcatctca acttcttcgc caaagcggtt aatagtgcgc
    36721 cccggcacgg taatgctgga ggtcggtttc agcgccatat gagcaatgat ttgctgcccg
    36781 ctgctgatac cgccgagaat gccgcccgca tggttgctct ggaaaccgtc tttggtgatt
    36841 tcgtcgcggt tctggctgcc gcgcagcgcc accacgtcaa agccgtcgcc aatttccacg
    36901 cctttcaccg cgttgatgct catcagcgca tgggcgatgt cagcatccag gcggtcaaag
    36961 accggctcgc caagtccggc aggaacgcca ctggcaacaa cggtgacttt agcgccgatg
    37021 gagtcgccct cttttttcag cgcacgcatc aactcgtcta acgcgtcgat tttgtcgggg
    37081 tccgggcaaa aaaacggatt ttgctcgacc tgcgaccagt ctttgatatc cagcggaatg
    37141 tcgcccatct gggtcaggca gccacggatt tcaataccaa atttctcggc gagatatttt
    37201 ttggcaatag ctcctgccgc cacgcgcatg gcggtttcgc gggcggaaga acgtccaccg
    37261 ccgcgataat cgcgcagacc gtatttttgt tcgtaggtgt aatcggcatg gcctggacgg
    37321 aaaacgtcct taatcgcact gtaatcctga gagcgctggt cagtgttttc gatcaacaag
    37381 ccaatgctgg tgccggtagt aacgccttca aaaacaccgg agagaatttt gacctgatcc
    37441 ggctcgcggc gctgggtggt atagcgcgat gtcccagggc gacgacggtc gaggtcatgt
    37501 tgcaggtccg cttccgtcag cggaatgcct ggcggaacac catcgacgat gcagccgagc
    37561 gccagcccgt gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat tgtgtttcca
    37621 gccatcacgg ctccgttatt gttgtgtttg cgtgtttact taatctttat aaatcgcgaa
    37681 atgttcacgg gccgccagca gctgctcttt ggtgagcata aacacaccat cgccgccgtt
    37741 atcaaactcc agccaggtga acggaacatc cggatattgt tccataagat gtaccatgct
    37801 gttgccgact tcacaaatca gcacaccatc atcagcaagg taatctgccg cgttaccgag
    37861 aatgcgacgc gtcagtttca ggccgtcggt gccagatgcc aagcccagtt ccggctcgtg
    37921 acggtactca tttggcaggt cggacatatc ttccgcatcg acgtacggcg ggttagtgac
    37981 gatcaggtcg tactgtactt tcggcaaatc acggaacaga tcggagcgaa tcgggatgac
    38041 attgtggatc agaccgtgtt cttcgatgtt ctgttcggca accgccagcg cgtcgggaga
    38101 gatatccacc gcgtcgactt ctgcatccgg gaaggcataa gcacaggcaa tggcgatgca
    38161 gccgctacca gtacacatat ctaaaatatg ctgcggttgc ttgctgataa gtccggcaaa
    38221 tttattgttg atcagttcac caatcggcga acgcggcacc agcacgcgtt catcgacgta
    38281 aaattcatgg ccacagaacc aggctttgtt ggtcaggtag gccaccggaa tgcgctcgtt
    38341 gacgcggcga atcacgcgct caacaatacg gtgtttttcg ctggaggtca gacgcgcggt
    38401 gcgcatgtct tccgggatat ccaacggcag gtagagcgaa ggcaacacca gttgcacggc
    38461 ttcatcccac gggttgtcag tgccgtggcc gtaccagata ttcgccgcgc tgaagcggct
    38521 caccgaccag cgcaacatgt cctgaatggt ttgcagctca tttactgctt catcaacgaa
    38581 aattttatcc acgtattcct ccagggcatg atcgcaataa tttcggcggc tagtttgcca
    38641 tgaagatgac gataaatcag cattcacgcg cggtgagtga ggaaaaatac gtttaaaacg
    38701 atcgattgcg ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga cagataaatg
    38761 aaaaagaaaa caacacttag cgaggaggac caggctctgt ttcgccagtt gatggcgggg
    38821 actcgcaaga ttaagcagga cacgattgtc caccgaccgc agcgtaaaaa agtcagcgaa
    38881 gtgccggtga agcggttgat tcaggagcag gctgatgcca gccattattt ctccgatgag
    38941 tttcagccgt tactgaatac cgaaggtccg gtgaaatatg ttcgcccgga tgtcagccat
    39001 tttgaggcga agaaactgcg ccgcggcgat tattcaccgg aattgttttt ggatttacac
    39061 ggtctgacgc agctgcaagc taagcaggaa ctgggcgcgt tgattgccgc ctgccgccgt
    39121 gaacatgtgt tttgcgcctg cgtgatgcat gggcacggga agcatatttt gaagcaacaa
    39181 acaccgctgt ggctggcaca acatccgcat gtgatggcct ttcatcaggc accaaaagag
    39241 tatggtggtg atgcggcgtt gctggtattg attgaagtgg aagagtggct gccgccggag
    39301 ttaccctgag aagtttagcg cgttgcgcca tgtacgggga atggcagggt caaatgaccc
    39361 tgccttgtga aaaagacgca ctagtacata ttcagtatgt aagtgaaccg gcaggtggca
    39421 tctattggct gttgcgccgt tgtgtccacg cgtagcttca tatcatgcag ttgcctggac
    39481 gtgatcggtt gcggctctgg cgtcactgtc actttggcat cgtcacagcc cgaagtggag
    39541 aaggtcatct gctgtgcgac aaaacggcag taagcaccac gctcgtcata tttactacca
    39601 tcacacagtt ttccaccttc catcagataa gtgggcagcg tttgttcaat cacagcacct
    39661 gttgattgca tcctgactat tttgttatgc acgaccgtat tttcattcca ggagacatca
    39721 taaggatcta aaggtattgc gctacagtca gaactgactt ttgtcgggcc aatataggga
    39781 acattattac ctgtatatat tgcctccgtt gcgtcctcac tctttacata cgaggaaact
    39841 atccacggac aaccatgcat atcaccgtca tagaaaactg tgactttcat ttccacgcag
    39901 gcccaggtcg tttgtggggt gcgagcatca tattcttcaa ggcccgggca acgtgaagtt
    39961 ggtaatatgg ttcttgcgtc ttctaaatgc agttccggag cagtaccttg accagtccat
    40021 gatactagtg gcacattcaa aatcgcaact gacctggatt gatatacccc atattgcgcg
    40081 ccttctggtg tgaggataga aagattaaac tgaaaacctg tgagcactcc cattgatccc
    40141 gcaggaatgt ataacgtcgc gatctttttc gcggcctgca ctcctgtgct gaggcaaatg
    40201 agcagcaaaa agtaaaagac ataacgtaga attttcattt taaatatact cgaaggtagc
    40261 ggtcaggctg gcagtgaatt caccgatcgt cacatcgcga ccgcttttcg cctggatcca
    40321 ggcccggaaa ttaatatcat tgttgccgtt ggtaagcgca aaagtcgccc cggtagtgtt
    40381 gttaatatcc acccgcttgc cgtcgagcgt ttccatccct atccccacgc cctgcgcggt
    40441 ggaggtgtca tcgactgcca gaaaacccgg ctgaccgctg tcttcggtgc cgctcagcgt
    40501 gactttgacg ttgtagcccg ctggcccttt gcagtctttc agcttaaaca caaccggcag
    40561 atgcacagac tgtccggcga ctatcaaatc ccggttgctg acggtgggaa aatggatttc
    40621 tgccagattg gcaccatcca ccaccagcgt gcaggattta ctcagcaggt tgccgtaaaa
    40681 atgcaggttg ttatcgactg cccgtgcccc cgtgctggcg gtcagcagca ggcctgctaa
    40741 cagcgcataa ccgggtgaat aaatgtgtgc tgttttcatt ggtaatccac ccgtaaggtt
    40801 gcgtaagcag aaaaggtcgt gtccgtcagc accgcatcag ccgctttgac cggtaccgcc
    40861 tccagctttg gcttctggct aaaatcggtg gcgttaatgg aaagcggctc gttgagctta
    40921 aaacgctgac cgtcatgctg aagctcaatt ccgaagcccg gtacatccgt ctcaatagca
    40981 gagtcgttgt aggaggtggc agtccccgtc catgagagtg tcatattcca ctcgggatcg
    41041 cggacatcag actcacagct cagttcgtag tccacttcct gacgaccata attgccgtca
    41101 atgctgtcga taatgagatc gcgaaattgc acttcgatgg tttgcccgcc gctgatgcta
    41161 caggcgggcg gcgacatcag cgtaccgtgg aatgtgatgt catcttccgc cgccagcaca
    41221 gccccgcaac cagcgcacag taaaaggagc aggaattttt tcattggtaa tccaccacca
    41281 tggtcatcgt ggcgttaaac tccgacggcg tcagttgcac cccgctctgt tttaccggca
    41341 ccgctttcag ctgaggctgt gtgttgacat taaaaggcgt ccagttattt tcaccaactg
    41401 caaacaggga gttgtcggcg gcgttttcta tccgcacccc caggccggcg atatccgtgc
    41461 tgagaaccgt ttctccgttg atcacggcgg tattgccttt gagctgcatc cgtaagtcat
    41521 cgctgacttt attaccgcag ttgagggtgt atttcgcatc ctgttggtaa ttggtaccat
    41581 cgatcctgtt gatcaccaca ttgccaaatt ccaccgcgct tcccttcacg gagcacggcg
    41641 gcgggccatc gaccaggatt cgcagcgtca gatccacctg tttgatatcg ctgccagcaa
    41701 cggctaaacc gctgacaccc catccggcca gtaccagtag ccccgcgccc gctaaaaact
    41761 tgtgactcat aagtccctcc cggcatcagc ctttgcggct ctgctcttca tccacctggc
    41821 agttgttgcc ggtgcatttg aagtacagcg gcatccgcgc gccgtagtcg ttgatgtagg
    41881 tcagcaccgg cacgctgtcc atcttcgcat tcagcggtgc ggaggttttc ggctccatca
    41941 tcagcggtgt gaatcccgcc ggcgggttgc cgcctttttt agcgctggcg ttgctgatga
    42001 tcacgtagta cggcgtcggg ttattgacgg tgaagtcctg gccgttacgg atgagcgtca
    42061 ccttatgctg ccacggactt ttcatgctgg tgttctccag cgccttcggt cgccagaaca
    42121 gtttgatacg cgtctgcagc gcaatctgca gtgtgttcgg tttatttgat ttcggcggaa
    42181 tttcgcgcac gttgaaatag aacacgctct cgcggtcggc cggcagtttg ttgatatccg
    42241 gcagcccctg caccttcacc tggccgttca tcatcgcatc gatgcgctgc actggcggaa
    42301 gcaccgcaag cggcgagctg attttgttgc cctttgcatc ctctatccag ctctgcgcca
    42361 gataaggcag tttgggatcg ttgttgcgca gcgtgacgct gatggacttg tcgctttcgt
    42421 taaacaccag ccgtgtcctg tcgggcgtca ccgaagcgtg gctggagaaa ctgaccgctg
    42481 tcgccagcag cgccagtgcc gggacaacga tttttgcagt gttagtcaga ttaaacatgg
    42541 atcattcctt aatcacgtta ctggtttact gaaattgaag tcgggggttc cgttggcgtc
    42601 acccgctgtg tctgttcctg aatcgccggt ttgaccgccg gcgaagtgac aggtgctaca
    42661 ttgcctttat gctggcatgg cagtaacaag ccgttaaaca gatcggcagg cagcgggtcc
    42721 ggcaggttga tatcgcaatg cgcaacacca ctccagaaca cactcatgtg ttcgccaggt
    42781 ttcacccctg ccagataaac attgccgtca tcatcgacaa ggcccactgt ctgctcgtta
    42841 tcatttttta cttctgcgcc aaacggagga tggctgccgt cgctcaggcg cagcaccgcc
    42901 atcgcttttt gaccactgat gacggtaaat ttgcggtagc caatcgcccc ttcagttagc
    42961 gtggcttgta ccaccgactg ggttgcttcg gcgttttccg gcagtttgtt gaggtcgata
    43021 tacgcctgat tgcggtaata gttattgacg tcagaaacga cggccttacc aaacatattg
    43081 gtataaacag ctgccccgtt accttccacc ggaacatcgg caacgccatc ggcatcaatc
    43141 aacaagcgtg tcccgcccat attctgggta cggtgaagtg cgccaccgtg ggcagtaagc
    43201 gttgcgccgc cctgtaacga caagcccgca gaggtgtatt gcccttcatg gtagttcgcg
    43261 ctgaggtcaa cctgcgccag cgaaccatca tggctgtaat agccgtcaac gctggtgtgt
    43321 ttgtcactgg tgccgacgtt caactgatag tgagtcgcgt catcgacacg gctgaaataa
    43381 ccgacctgac tgctgtccgt cccactgcca tagttgccgt tatagctaac ggtactgttg
    43441 tcgccccacg gcatactgag cgaaatgtac atgcccttat ccgcccggtt gtcatactcg
    43501 tagcggtagc cagtcaggga aacgctcata ttacgaatgc tgcccatatt gaaatagtgg
    43561 gagagcatga tgttgtagtt tgtctgctcc tcacgatccc agtaagtatg gcgggtatag
    43621 ttgagataaa ccgaaacacc cgcatcgcgg aagttctggt tataagtggc ggtgtacatc
    43681 tctttgtcgt tgcccgtgcg taccattccg ctgtcactgg catccagata ctcgctcatg
    43741 gtcataaagt tctcttccga gaagcgatat ccagcaaaag taacgcggct gttgagctgg
    43801 tcaaagtctt tggaataact cacacggaag gagttaccgt ccagcgaacc tttgccataa
    43861 gcggtgtctt tatccagttt ggtgtgcgag tgagtaacat caaacgcgac cgcgccgaac
    43921 gtagacaaat cgcgaccaac gccaagtgcg gcagattggt agttttcatc ccccagcgcg
    43981 ccaccataga gcgaccaacc attagcgatc ccccaggaag cttcagcacc agagaaaaat
    44041 tcgccttcaa catgatgccc ccactcttgc ggacggccca tcatgatctt atagcgaacc
    44101 tgacctggac gagtgaggta tggcatcgag gcggtgctga tgtcatattc ctgcacctgg
    44161 ccgttctgtt cttcaatgcg aatatgcaac gtaccggaga cagaatcacc aagatcctga
    44221 atacgaaacg gtccagccgg aacctgcgtt tcgtaaatca cacgccccat ctggctgacg
    44281 gtcacttttg ctgtggtgtg tgccacgccg gaaatgtctg gcgcgtagcc gcgcagattg
    44341 ggaggcaaca tttgatcgtc ggtgctaacg ctgccaccaa catagttaaa gccatcaaaa
    44401 atatcggatc tgaggtaatc ttcgcccagc gccagtttgg cttttaatga cggtaacgcc
    44461 cgccaggcat agtagcgact ccactcccat tttttttgag tttcatcgcc gctgaattca
    44521 tcgccatcat cattactgcg agtatgttga tagttagtct gccagtcagc acgcatacgc
    44581 cacggcccca ggttaacccc gaccgtcccg ttgccgctga tctcgttact atcatcaccg
    44641 ccattttctt cgtgccgggt ttgtgcgttg atgctgtagt ccgcgacgat cccggagatg
    44701 ccgtcatccc aacgtgaagg cggatcccag tcgggccagg tatattcgag gtaagcctgc
    44761 ggtagtgaaa tgactaatgc ggactggctt aaatcagcct taatttccat gccttccagt
    44821 tgaccggatt tcaggcattt agcatcgtgg ctccattgca gatttttcgc cacgtcttct
    44881 tttaaaccaa actgcgctac cagttccggt gtcagacaag catagctttt gctcgcgtca
    44941 tcttcaccag cataccagta aatatcgtac tcttccgcca atggctgttt atttagttga
    45001 acctgtaaat tgtattttcc gggctcaaca tatccctggc tggaaaaacg cttcagatca
    45061 atttttgtgt cgccttttaa ttccagaaaa cgggaatcaa actgaatgtc gtcttcagcc
    45121 cagacactgc tataactccc tgacattgcc agcgcaatgc accacggaag aatccgtaat
    45181 cgaaaaagag aatggtcagg catacacgtt tctatccgtt tttattcttg ttcggtcaaa
    45241 atccatttaa atcccccata gcggtgtcgc tatgggggta tgccttacag gtaagtaatc
    45301 tggaaggtgg tatttgcttc gaaattaccc agatctgggg catcagctgc gccgaccagc
    45361 caggctttaa agttcagtgt ctgcttcgct ttgcctttat tggtagaagt gtctttcact
    45421 attttctggt caatacccat gccgcttttg taagaggtac ccagagagtc accgatcgcc
    45481 aggctaacat tgttaaatgc cgcaccggta tcggtattga aaatggtgta ataattgccg
    45541 ctatttgcag aagaaacggt accagtaaag gtagtggtca ttgtttcctg agtatcaaat
    45601 acgcaatctt gcagacgaat ctggaaactt ttcggcgtgg tggtgccgtt atttttcagt
    45661 ttttcggcac cgatatcacc catatcaact tccagtttgt gatcgtccgg gacgatagaa
    45721 cacactgagt cttcaatggt gccgtaaaaa cgtgcagtac cgttgttacc cgcagcgatt
    45781 gttgctgtag aggtgaacgc gcccatcacc attgccgcag caatagctgt tttaacaaac
    45841 ttactcatta tttgtcctta aactataaat aattaaaaat ataaacattg caatacattg
    45901 attcagtcaa tagccaatgt ttatacccag ccattgaata cggcatgcga tccaaaaaga
    45961 acaggtcgta gaaaattgta ttcgttggtg tggggccgca tagtgatgtt tccacacccc
    46021 tgatgcaatt taattttact aaagagtaaa tgtagattta attaatatat tgacagaaga
    46081 aaggttatct taaggacatc cttaaaaaat acggaataag tatttagaaa aaatagaaag
    46141 tatatcaata atgaatatca acagcttaaa aaacaaacaa tgaacatata at
//
LOCUS       DAOFQD010000023        45270 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.023, whole genome shotgun sequence.
ACCESSION   DAOFQD010000023 DAOFQD010000000
VERSION     DAOFQD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.023"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            58..660
                     /locus_tag="QBF46_001367"
     CDS             58..660
                     /locus_tag="QBF46_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDQ6673569.1"
                     /translation="MLAFCRSSLKSKKYIIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(699..1124)
                     /gene="nudI"
                     /locus_tag="QBF46_001368"
     CDS             complement(699..1124)
                     /gene="nudI"
                     /locus_tag="QBF46_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDQ6673570.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWAISGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1404..1946
                     /locus_tag="QBF46_001369"
     CDS             1404..1946
                     /locus_tag="QBF46_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673571.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIETGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2046..3248
                     /locus_tag="QBF46_001370"
     CDS             2046..3248
                     /locus_tag="QBF46_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDQ6673572.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLKLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3468..4250
                     /locus_tag="QBF46_001371"
     CDS             3468..4250
                     /locus_tag="QBF46_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDQ6673573.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4265..5470
                     /gene="rhmD"
                     /locus_tag="QBF46_001372"
     CDS             4265..5470
                     /gene="rhmD"
                     /locus_tag="QBF46_001372"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HDQ6673574.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLSATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5527..6816
                     /locus_tag="QBF46_001373"
     CDS             5527..6816
                     /locus_tag="QBF46_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6673575.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6834..7637
                     /gene="yfaU"
                     /locus_tag="QBF46_001374"
     CDS             6834..7637
                     /gene="yfaU"
                     /locus_tag="QBF46_001374"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDQ6673576.1"
                     /translation="MNALLSNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     EQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7678..7875)
                     /locus_tag="QBF46_001375"
                     /pseudo
     CDS             complement(7678..7875)
                     /locus_tag="QBF46_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7876..8775)
                     /locus_tag="QBF46_001376"
     CDS             complement(7876..8775)
                     /locus_tag="QBF46_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDQ6673577.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDEPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLATTQLTDADIPNCH"
     gene            complement(8969..10159)
                     /gene="glpC"
                     /locus_tag="QBF46_001377"
     CDS             complement(8969..10159)
                     /gene="glpC"
                     /locus_tag="QBF46_001377"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDQ6673578.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10156..11415)
                     /gene="glpB"
                     /locus_tag="QBF46_001378"
     CDS             complement(10156..11415)
                     /gene="glpB"
                     /locus_tag="QBF46_001378"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDQ6673579.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVKTAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11405..13033)
                     /gene="glpA"
                     /locus_tag="QBF46_001379"
     CDS             complement(11405..13033)
                     /gene="glpA"
                     /locus_tag="QBF46_001379"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDQ6673580.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13306..14664
                     /gene="glpT"
                     /locus_tag="QBF46_001380"
     CDS             13306..14664
                     /gene="glpT"
                     /locus_tag="QBF46_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDQ6673581.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14669..15745
                     /gene="glpQ"
                     /locus_tag="QBF46_001381"
     CDS             14669..15745
                     /gene="glpQ"
                     /locus_tag="QBF46_001381"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDQ6673582.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(15787..16029)
                     /locus_tag="QBF46_001382"
                     /pseudo
     CDS             complement(15787..16029)
                     /locus_tag="QBF46_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            16208..16858
                     /gene="inaA"
                     /locus_tag="QBF46_001383"
     CDS             16208..16858
                     /gene="inaA"
                     /locus_tag="QBF46_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDQ6673583.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(16912..17166)
                     /gene="yfaE"
                     /locus_tag="QBF46_001384"
     CDS             complement(16912..17166)
                     /gene="yfaE"
                     /locus_tag="QBF46_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDQ6673584.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(17166..18296)
                     /gene="nrdB"
                     /locus_tag="QBF46_001385"
     CDS             complement(17166..18296)
                     /gene="nrdB"
                     /locus_tag="QBF46_001385"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDQ6673585.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(18531..20816)
                     /gene="nrdA"
                     /locus_tag="QBF46_001386"
     CDS             complement(18531..20816)
                     /gene="nrdA"
                     /locus_tag="QBF46_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDQ6673586.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            21512..25246
                     /gene="yfaL"
                     /locus_tag="QBF46_001387"
     CDS             21512..25246
                     /gene="yfaL"
                     /locus_tag="QBF46_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDQ6673587.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVTDSCQGYDVKASCQ
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNT
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGISEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLTLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGSDGKNGVYVVVDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVDDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SEAVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGDGQTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGDLFSGRWGTDGEWMLGAVG
                     GYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFNNDV
                     SEHEDGVDRYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            complement(25387..26109)
                     /locus_tag="QBF46_001388"
     CDS             complement(25387..26109)
                     /locus_tag="QBF46_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDQ6673588.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            26256..28883
                     /gene="gyrA"
                     /locus_tag="QBF46_001389"
     CDS             26256..28883
                     /gene="gyrA"
                     /locus_tag="QBF46_001389"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDQ6673589.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            29032..30720
                     /locus_tag="QBF46_001390"
     CDS             29032..30720
                     /locus_tag="QBF46_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDQ6673590.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            30717..31340
                     /locus_tag="QBF46_001391"
     CDS             30717..31340
                     /locus_tag="QBF46_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDQ6673591.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            31364..35876
                     /locus_tag="QBF46_001392"
                     /pseudo
     CDS             31364..35876
                     /locus_tag="QBF46_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            35877..37142
                     /locus_tag="QBF46_001393"
     CDS             35877..37142
                     /locus_tag="QBF46_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDQ6673592.1"
                     /translation="MNWRRIVWLLALVTLPTLAEEPPLQLALRGAQHDQLYKLSSSGV
                     TNVSTLPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMAAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATA"
     gene            37170..37526
                     /locus_tag="QBF46_001394"
     CDS             37170..37526
                     /locus_tag="QBF46_001394"
                     /inference="COORDINATES: protein motif:HMM:NF021572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDQ6673593.1"
                     /translation="MLAFAYPDNILSRWGAPRSTCQLLPKAKAWLAKKMPQWRRILQG
                     ETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRNFFTLQDRLDLTHEYLHLAFDGYPTG
                     LDENYIETLTRQLLMD"
     gene            37531..38307
                     /locus_tag="QBF46_001395"
     CDS             37531..38307
                     /locus_tag="QBF46_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDQ6673594.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(38381..39565)
                     /gene="atoB"
                     /locus_tag="QBF46_001396"
     CDS             complement(38381..39565)
                     /gene="atoB"
                     /locus_tag="QBF46_001396"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDQ6673595.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITRDMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(39596..40918)
                     /locus_tag="QBF46_001397"
     CDS             complement(39596..40918)
                     /locus_tag="QBF46_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDQ6673596.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(40915..41565)
                     /gene="atoA"
                     /locus_tag="QBF46_001398"
     CDS             complement(40915..41565)
                     /gene="atoA"
                     /locus_tag="QBF46_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HDQ6673597.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(41565..42227)
                     /gene="atoD"
                     /locus_tag="QBF46_001399"
     CDS             complement(41565..42227)
                     /gene="atoD"
                     /locus_tag="QBF46_001399"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDQ6673598.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(42422..43807)
                     /gene="atoC"
                     /locus_tag="QBF46_001400"
     CDS             complement(42422..43807)
                     /gene="atoC"
                     /locus_tag="QBF46_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HDQ6673599.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETNCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKNTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIGATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(43804..>45270)
                     /gene="atoS"
                     /locus_tag="QBF46_001401"
     CDS             complement(43804..>45270)
                     /gene="atoS"
                     /locus_tag="QBF46_001401"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HDQ6673600.1"
                     /translation="QNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMLPIER
                     NGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANIDIIT
                     DGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVIAIDR
                     QGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALEISFP
                     GRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGELMAGV
                     AHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRPRHSQ
                     WQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNILINAV
                     QAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGLGLAL
                     SQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
BASE COUNT        10833 a        11170 c        12255 g        11012 t
ORIGIN      
        1 atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
       61 ttagcttttt gccgctcttc gttgaagtca aaaaaatata tcatcatttt actggcgctc
      121 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      181 gacaataaaa cactggccag actggcacag cagcacccgg ttgtcgtttt gtttcgtcat
      241 gctgaacgtt gcgaccgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      301 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gtgctgatat ccctgatttc
      361 gatctttatt ccagtaatac cgtccggacc attcagtcgg ctacctggtt ttcagcgggt
      421 aaaaaattga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcaatt
      481 aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      541 ctgacatata ttgctaaaga taagcgtgac gcgacattta aacctgatta tctggatggt
      601 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      661 attttcaatc tgacagccag taatggctgt catcattgtt acagaagacc tttcaaacgt
      721 aacgtttttc gggtggcgac attcaaatca taatgcacca gatcttcagg ttttacccac
      781 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      841 atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt ggtgcgaata
      901 tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
      961 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag gctccacgcc gccacccgaa
     1021 atcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag caaataagca
     1081 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcacaggagt attccttata
     1141 aataaaaaag aaaccatgag aatacggtaa ctcagaaata tgtgaaagtg acagtaatct
     1201 cttttgcgat gataactgca cttattatta tttttgtgtg tatcgataaa gtgtgtttat
     1261 tttttcggcg tttaattatt taagaagtat ttagttacaa taagtgacta gatccctttt
     1321 ttcgctcatt gaatcctctg tgccaggcag tacaatcccg cagctttggg aattttgatt
     1381 aattgcttgt gggaaagggg ataatgaaaa aaattgcgct agcaggtctg gccggaatgc
     1441 tgttggtttc tgcatcggtc aatgcaatga gcatcagcgg ccaggcgggt aaagaataca
     1501 ccaatattgg tgtcggtttt gggactgaat cgacgggcct ggcgttaagc ggtaactgga
     1561 cacataacga cgacgacggt gacgtcgctg gcgtggggct ggggttgaat ctgcctctcg
     1621 ggccgttaat ggcgaccgtt ggcggaaaag gcgtttacac caacccgaat tacggcgatg
     1681 aaggttatgc cgcagcggta ggaggtggtt tgcagtggaa aattggcaac agcttccgtt
     1741 tgtttggcga gtattactac tctccggatt cgctctccag cggtattcaa agttatgagg
     1801 aagcgaatgc tggcgcgcgt tacaccatta tgcgtccagt cagtattgag acgggttatc
     1861 gctacctgaa tctgtcgggt aaagacggta accgcgacaa cgccgtggct gacggcccgt
     1921 atgttggggt taacgccagt ttctgatccc ttccggcgcg gcattctgtc gcgccattca
     1981 gtttcttact acgccttatc tccctttgcg ttatagtgat tcgataacaa aagcaggaga
     2041 taaccatgtt aaaagtggaa atgttatcca ccggggatga agtgttacac gggcaaatcg
     2101 ttgacactaa cgctgcctgg ctggccgatt ttttctttca tcaggggctg ccattatctc
     2161 gccgcaatac ggtgggggat aaccttgatg acttagtcac cattcttcgc gaacgtagtc
     2221 agcacgccga tgtgctgatc gttaacggcg ggctggggcc gaccagtgat gatttaagcg
     2281 cactcgccgc tgcgacagca aaaggtgaag gcctggtgtt gcatgaagcc tggctcaaag
     2341 agatggaacg ctatttccac gaacgtggac gagtaatggc accgagcaac cgtaaacaag
     2401 cggagctgcc tgccagtgct gaatttatca ataacccggt aggcaccgcc tgtggttttg
     2461 ccgtgcagct taatcgttgc ctgatgttct ttactcccgg cgtaccgtca gaatttaagg
     2521 tgatggtcga gcacgaaatc ctgccgcgtc tgcgcgagcg tttttcttta ccacagccgc
     2581 cggtttgtct gcgtttaact acctttggtc gttcggaaag cgatctggca caaagcctgg
     2641 acactctaaa actgccgccg ggcgtaacaa tgggctatcg ctcctcaatg ccgatcatcg
     2701 aactgaaact caccggaccg gcaagcgagc aacaggcgat ggaaaaactg tggctggacg
     2761 ttaaacgtgt tgccggacag agcgtgattt tcgaaggcac cgaaggactg cccgcgcaga
     2821 tcagtcgcga attgcaaaac cgccagttca gcctgacgtt gagcgagcaa ttcaccggtg
     2881 gtttattggc tttgcaactt tctcgcgcag gtgctccatt gctggcgtgt gaagtggttc
     2941 cttcacagga agaaaccctg gcgcaaactg cgcactggat tacagaacgg cgggccaacc
     3001 attttgccgg gctggcactg gctgtttcgg gtttcgagaa cgagcatctc aactttgcgc
     3061 tagccacgcc agacggcact ttcgctctgc gtgtgcgttt cagcactaca cgctacagcc
     3121 tggctatccg tcaggaagtg tgcgcaatga tggcactgaa tatgctacgc cgttggttaa
     3181 acggccagga catcgccagt gagcatggct ggattgaggt tgttgagtcc atgactttat
     3241 ccgtctgatt tcgccactct gtgccggttt actgcaccgg cacattcccc ctccggcaaa
     3301 atttgtccaa cctgttgtac tcaataagaa aaaatccgtg cgctgattca caaatttgtg
     3361 cgtaatttct ccagtccgct ggacgaggag cgggatttga tgcgcttcac aattttactc
     3421 cccctgagca acgagactga ttccagtatc taagactgga gtccagtatg ttggaatcaa
     3481 gcaaagttcc cgccctgaca cgggctattg atattctcaa tttgattgcc cgcattggcc
     3541 cctgtagtgc tgcgacgatc attgacacat tgggaatccc taaaagcacg gcctatttgc
     3601 tgcttaatga actcaggcgt cagcgttttc ttagccttga tcaccaggaa aatttttgtt
     3661 tgtggaccag gctggtggag ctttccggtc atgcgttaag caagatggat ctccgggaac
     3721 tggctcgacc gcgtttgacg caactgatgg ataccaccgg gctgttatgt cacctgggga
     3781 ttattgataa cggcagcgct tattacattt tgaaagtgga gtcatcagcc actatcagcg
     3841 tgcgttccca tgaaggaaaa agcctttcgc tttatcgctc cggaataggt aaatgcctgc
     3901 ttgcctggca acctgcagcg gtacagcaaa gtattatcga agggctggtc tgggagcagg
     3961 cgacacccac aacgattact catccacagc aacttcacga agaactggcg cgtattcgcc
     4021 gtcagggctg gagctatgac aacggcgaag attacgccga tgtccgttgt gtcgctgcgc
     4081 cggtgtttaa cgccaataac gagcttaccg ctgcgatctc ggtggtcggt acccgcttac
     4141 aaatcaatga agaataccgt gattatctgg cgggcaaggc cattgcctgc gccagggata
     4201 tttcacgtct gttgggatgg aaaagtccct ttgacttaca agcctcataa caatggagaa
     4261 catcatgacc ctaccaaaaa ttaaacaggt tcgcgcctgg tttactggcg gtgcgacagc
     4321 agaaaaaggc gctggcggcg gtgattatca cgaccagggg gcgaatcact ggatagacga
     4381 tcatattgcc accccgatga gtaaataccg cgattacgag cagtcacgcc agtcatttgg
     4441 cattaacgtt cttggcacgt tggttgttga agtcgaagca gaaaacggcc agaccggatt
     4501 cgccgtttcg acagccggtg aaatgggctg ttttattgtc gaaaaacatc ttaaccgttt
     4561 cattgagggt aaatgtgtca gtgatatcaa actgatccac gatcaaatgc tcagtgccac
     4621 cctgtattac tccggctctg gtggcctggt gatgaatacg atttcctgtg tcgatctggc
     4681 tctgtgggat ctgttcggca aagtggtcgg gcttccggtt tataaacttt taggcggcgc
     4741 tgttcgtgat gagattcagt tctacgccac aggtgcgcgt ccggatctgg caaaagagat
     4801 gggctttatc ggtggcaaaa tgccgacgca ctgggggcca catgatggcg atgcggggat
     4861 ccgcaaagat gccgctatgg tcgcggatat gcgtgaaaaa tgcggtgagg atttctggtt
     4921 aatgctcgac tgctggatga gtcaggacgt gaactatgcg accaaactgg cccacgcttg
     4981 cgcgccctat aacctgaaat ggatcgaaga gtgcctgccg ccacagcagt atgaaggtta
     5041 tcgcgaactg aaacgcaacg cgccagtcgg aatgatggtc accagcggtg agcaccacgg
     5101 cacactgcaa tcttttcgta cgctttcaga aaccggtatc gacattatgc agccggatgt
     5161 tggctggtgc ggtggtttaa ccacgctggt ggaaattgcc gcaatcgcca aatccagggg
     5221 gcaactggtg gtgccgcacg gttcgtctgt ttactctcac catgcggtga tcaccttcac
     5281 caatacgcca ttcagcgaat tcctgatgac cagcccggat tgttcaacga tgcgtccgca
     5341 gtttgacccg attctgctca atgagccggt tccggtgaat ggtcgtattc ataaatcagt
     5401 gcttgataaa cccggttttg gcgtcgaact caatcgtgac tgcaatttga aacgccccta
     5461 cagccactaa tcactccggg cgttgctgtg acaacggtaa cgcccacata ttctaaggag
     5521 ctttttatga gcaccgcttt gcttgacgcc gtggtgaaga aaaaccgtgt gcgtttaatt
     5581 ccgtttatgt tggcgctgta tgtgctggcg tttctcgacc gttcgaatat cggttttgcc
     5641 aaacagacct accagattga taccgggttg agtaatgaag cttatgcgct gggagcaggc
     5701 attttctttg tggtatatgc gtttctgggt gttccggcga atcttttgat gcgcaaactg
     5761 ggggccagaa cctggattgg tacgacaaca ctgctgtggg gatttctttc ggcagccatg
     5821 gcatgggccg atactgaagc gaaatttctg atagttcgca ctctgcttgg tgctgcggag
     5881 gccggatttt tccctggtat gatttatctc acctcgcaat ggtttccgca gcgtaatcgc
     5941 gccagcatta tggggctgtt ctatatgggc gcaccgctgg cgttaacact gggatcaccg
     6001 ctttccggcg cgctgttgga gatgcatgga tttatggggc atcccggctg gttctggatg
     6061 tttgtgattg aaggattgtt ggcagtcggt gctggggtat tcacattctt ttggcttgat
     6121 gacacaccgg agcaggcacg ttttctgagt aaacaagaaa aaacgttgct tatcaatcaa
     6181 ctggcaagtg aagaacaaca gaaagtgact tctcggctga gcgatgcgct gcgtaatggc
     6241 cgagtctggc aactagcgat tatctacctg accattcagg tggcggttta cggattaatt
     6301 ttcttcctgc cgacccaggt tgcggcattg ctgggaacaa aagtgggctt tacagcgtcg
     6361 gtggtcaccg ccattccgtg ggttgcggcc ttgtttggga cctggcttat tccgcgctat
     6421 tccgataaaa ccggtgaacg gcgtaatgtc gcagcgctga cattactggc ggcgggcatt
     6481 ggtattggtc tgtccgggct gctttctcca gtaatggcga tcgtagcgct gtgtgttgca
     6541 gctatcgggt ttattgccgt gcagccagtg ttctggacga tgccgacaca actgctttcc
     6601 ggtacggcgc tggctgcggg aattggtttt gtaaacctgt ttggtgcagt gggcgggttt
     6661 attgccccga tcctgcgcgt gaaagcagaa acgttatttg ccagcgatgc ggcgggatta
     6721 ctgacgctgg cagcggtggc ggtcatcggt tcgctgatta ttttcactct gcgtgtaaat
     6781 cgcactgttg cgcagaccga cgtggcacat cattaaatag gttaaggaac acgatgaacg
     6841 cattattaag caatcccttt aaagaacgtt tacgcaaggg cgaagtgcaa attggtctgt
     6901 ggttaagctc aacgactgcc tatatggcag aaattgccgc cacttctggt tatgactggt
     6961 tgctgattga cggggagcac gcgccaaaca ccattcagga tctttatcat cagctacagg
     7021 cggtagcgcc ctatgccagc caacccgtga tccgtccggt ggaaggcagt aaaccgctga
     7081 ttaaacaagt cctggatatt ggcgcgcaaa ctctactgat cccgatggtc gatactgccg
     7141 aacaggcacg tcaggtggtg tctgccacgc gctatcctcc ctacggtgag cgtggtgtcg
     7201 gggccagtgt ggcacgggct gcgcgctggg gacgcattga gaattacatg gcgcaagtta
     7261 acgattcgct ttgtctgttg gtgcaggtgg aaagtaaaac ggcactggat aacctggacg
     7321 aaatcctcga cgtcgaaggg attgatggcg tgtttattgg acctgcggat ctttctgcgt
     7381 cgttgggcta cccggataac gccgggcacc cggaagtgca gcgaattatt gaaaccagta
     7441 ttcggcggat ccgtgctgcg ggtaaagcgg ctggttttct ggctgtggct cctgatatgg
     7501 cgcagcaatg cctggcgtgg ggagcgaact ttgtcgctgt tggcgttgac acgatgctct
     7561 acagcgatgc cctggatcaa cgactggcga tgtttaaatc aggcaaaaat gggccacgca
     7621 taaaaggtag ttattgatat caaaggccca tggggatcgg ctgtgggcct gtgttaatta
     7681 gtggttattc gctgccagat cggcttcgct tagctgggtg gccgcgagca cctggtcacg
     7741 atcaaagccc tgttccagca tgcgcaaagc aattttaatg gcttgttcat gcaaaccttc
     7801 taatttacct tcctgccagc caatttgatg tccttcctgc cgcagccgtt cagcaatagt
     7861 cattaatctc tccttttaat ggcagttcgg aatatcagca tcagtgagtt gggttgtcgc
     7921 cagcacaatc tcacgttcaa agccctgctc cagcatgcgc aaagcaattt taatggcttg
     7981 ttcatgcata ccttcctgcc agccaatttg atgcccttcc tgccgtagcc gttcagcaat
     8041 agtcattaat ctctcctttt gtagtggtga acgttcggca atctcctcaa taaaacgggt
     8101 gaaacgggag gtatcgccgc attgcagcag ataattaaac agtgtttgta gctggctgtc
     8161 attagtgaag cctctaacca aaagcgtggt gatcctgtcg accatgccga ttaaatcgcg
     8221 gtcgcgaata tgcttttgaa tcagttccag cagagctata cgccgatgtt gcatgatctc
     8281 atcgtcaggt acgatggtga tatccaccaa cggaaaagct tcggtgtaca actgccgggc
     8341 aagttgcggc tcgtcaaact catccagcca gttaagtgag tacgggtagg gcgatgtttc
     8401 gccatgataa aacagcaatg gcaccaccag cggaactctg tcatagcctt tatccaggtg
     8461 acgctgcatg gcggcagtgg catagcgcat tagccgaaaa gccatattct tttctgcaga
     8521 gctttgatgt tcaatcacgc agtagatata gccgtcaccg tcgctggttt ccacggacca
     8581 caaaacatcc gagtagtaag cgcgtaaact tttttcaata aaactagtgg gttccaggcg
     8641 taaggtttgc aggttgcaaa gcttgcgcag tggttctggt aaatgtattt cgagaaaatc
     8701 ccgtgcggtt tcgggtgtga acataaaggt tttaaacacc gcatcatgcg gggaggatgt
     8761 tgttgattcg gtcatggttg tccgtcacct gatatttatg atgacgcgac attacgtcta
     8821 tcccgggcct ttgacatctg tcagtttcag cctttgcgag acattttcag aggaattttc
     8881 aaagcgttgc aacgcataca tggtgcgtgg aatcgcgctc gcaaaaatgc ccgataagca
     8941 atgtgcttac cgggcatcaa aaggtcggtt aagccagcgc ctgggccagt agcgtaatcg
     9001 gatgttcgca gcgaaggctc gtggacatct caatctgcca tttgcaggtt tcgcagtcgg
     9061 tgatgaccag atccgcgccg ctctcttcta tctggcggaa cagtggcgca ccgatggctt
     9121 gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg cagcactggg
     9181 aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg gtgtagagcg
     9241 tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt
     9301 tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtt gccagttcga
     9361 tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg tcgcgcaggg
     9421 caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc acagcttcgc
     9481 ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga
     9541 ttagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta cccattgcgt
     9601 tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg aagcagccgt
     9661 gaaagaaagc gacctggtct ttatattgtg cttgctgagc cgctatgctg cgataccagc
     9721 gacggaacgt gccgaaggag tatttcggta gcgtgcggcg atgatcgatt tttaacgccg
     9781 catcaagcag ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg
     9841 gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattacgc agcgacgggc
     9901 gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc gccaatcttc acatcggacg
     9961 ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg tcatacagtg
    10021 cgccatcttt caaacgcaga cgctcgccat ccggcccggc ttgttttggc cctggataac
    10081 cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta atgcagtttt
    10141 cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg
    10201 taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc
    10261 cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg acgggcgtag
    10321 cgtctcatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa aatcgccctt
    10381 ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat
    10441 gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga gaaccgcgaa
    10501 acgtggacgt agcggaatat cggcgtgatt gcgggtccag atttcattca ctacgccatt
    10561 tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct ggcgcacaaa
    10621 ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa
    10681 aagcatcagt gaacaaggta gtttttcatt caaccaacgc cacagtttgt catcggccag
    10741 gccgaagcag gcgggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc
    10801 aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca ccgcgcgaaa
    10861 ttcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg cggttttaac
    10921 cgaaagatcc agttcgcgca acgaagccgc cgcaagatgc gcctggaaat ccatcagccc
    10981 gcttatcccc actacacata tcttcttcgc gggtagcggc cagacgggaa cttctggcga
    11041 acttaaccag gttgcacgca aagtgccgag cggggtaacg cgctgatggg ccagttccac
    11101 gctgccctgc aattgcgcac cgctttcagc gatcaatgcc tgcgcctggc aagcgagatc
    11161 gagcacgcgt tgtggttcga gaagactgta aggatgggct ggtgcctgct gacgcaaaga
    11221 ttccagtcca ctgtgaatgt ctgtcaccgg ttgaccatca ggcagatggc tcagcaaatc
    11281 cagcgaaccg gatgagaaat gcagcgcgct ttgaccacgc gtgacaatgg cacagcgcag
    11341 gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac
    11401 agtatcaaag cgcatctttc tgctccttct ccagaccaca cagtccctga taaacccagc
    11461 gggtaaattc gctttcgcgc agtgcgtctc cccaggcgat gggttgcacg cctttccagc
    11521 gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg acattaaacc
    11581 gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggtg cccatcccca
    11641 cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact
    11701 gcacttcacc cgcagttacc gcttcgcact cacataccag gctacggtgc agacggcctt
    11761 cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg cgcagcgggg
    11821 caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcttgtgaa ccaggcagtg
    11881 ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt gcggcatacc gcgtcggtag
    11941 cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg ataaatccgt
    12001 ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg ttacgtccgc
    12061 tcgggtcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc aaaatgcgcg
    12121 ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca acctcttctg
    12181 ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg gtgccaatca
    12241 gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg cagcggttga
    12301 tcacatgctg gttaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc
    12361 gaatgcgcag atcggcatat tcggcaatgt gttgccccca gatcccagcg gcattaacca
    12421 cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca cgaacaccgc
    12481 acaccgtcgc gccttcacga atcagccccg tgacttcatg agcggtaagg ataacggcac
    12541 cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat
    12601 ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt
    12661 gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg
    12721 cctggaagga gaggtcatct tccggcaggg tgataaacag gccgttggtt ggttcaacgc
    12781 agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg gccgattccg
    12841 catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac
    12901 cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc
    12961 gggcaatccc ggctcccgtt gcgccgccgc caatgataat cacgtcactt gattgcgagt
    13021 cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa
    13081 catgaattgt ttgatttcgc gcatattcgc tcataattcg aaagtggagc gtgatttcat
    13141 gcgtcatttt gaacattttg taaatcttat ttaataatgt gtgcggcaat tcacatttaa
    13201 tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct ctcactgaat
    13261 caggctgtta atcataaata agaccacggg ccacggaggc tatcaatgtt gagtattttt
    13321 aaaccagcgc cacacaaagc gcgcttacct gccgcggaga tcgatccgac ttatcgtcga
    13381 ttgcgctggc aaattttcct ggggatattc tttggctatg cggcttacta tttggttcgt
    13441 aagaactttg cgcttgctat gccttatctg gttgagcagg gattctcacg cggtgattta
    13501 ggttttgccc tttcggggat ctcgattgct tatggatttt cgaaattcat catgggttcg
    13561 gtatcggatc gctcgaatcc gcgcgttttc ctgcccgcag gtttgattct ggcggcggca
    13621 gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt gatgtttgta
    13681 ctgttgttcc tctgcggttg gttccagggg atggggtggc cgccgtgtgg tcgtactatg
    13741 gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcac
    13801 aacgtcggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg gttcaatgac
    13861 tggcatgcgg cgctctatat gcctgctttc tgcgccattc tggtggcatt attcgccttt
    13921 gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga gtacaaaaat
    13981 gattatccgg acgactataa cgaaaaagcg gaacaggagc tgaccgcgaa gcaaatcttc
    14041 atgcagtacg tactgccgaa caaactgctg tggtatatcg ccatcgccaa cgtgttcgtt
    14101 tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga ggttaagcat
    14161 ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg aatatgcagg tattccgggc
    14221 actctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg ggcaaccggc
    14281 gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa cccggcaggt
    14341 aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta cggtcctgtg
    14401 atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg tacggcagcg
    14461 ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc gattgttggc
    14521 tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg cggcagcatt
    14581 ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca tgaacaatta
    14641 ctgcaaaaac gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc ttagcatggc
    14701 gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa
    14761 aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc tgccagcaaa
    14821 agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga tgaccaaaga
    14881 cgaccatctg gttgttctgc atgaccatta cctcgatcgt gttactgatg ttgccgatcg
    14941 tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca cgctggatga
    15001 aattaagtcg ttgaaattta ccgaaggttt cgatattgaa aacggtaaaa aagtgcagac
    15061 ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct ttgaagaaga
    15121 gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta tttatccaga
    15181 aatcaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa aaacgctgga
    15241 agtgctgaag aaatatggtt acaccggtaa agacgataaa gtttatttgc aatgttttga
    15301 tgctgatgag ctgaagcgta ttaagaatga gctggaaccc aaaatgggca tggagctcaa
    15361 tctggtacag ctgattgcct ataccgactg gaatgaaacg cagcagaaac agccggatgg
    15421 aagctgggtt aattacaact acgactggat gtttaagccg ggtgccatga aacaggtggc
    15481 ggaatatgca gatggtattg gtccggatta ccatatgttg attgaggaga catcgcagcc
    15541 gggtaatatc aaactcactg gcatggtgca agatgctcag cagaataaac tggtagtgca
    15601 tccttatacc gtgcggtcag ataaactgcc tgaatacact cctgatgtga atcagttata
    15661 tgatgctctg tataacaaag cgggtgtaaa tgggctgttt actgatttcc ctgataaggc
    15721 agtaaaattt cttaataaag agtaatgtta ctgcaggatg gcgttcatgc gccatcctgt
    15781 ctcatatcaa atttttccaa tcgccactaa ttgttccatg cacatttgta gtaaaaacag
    15841 tggactgcct tctaccggct tttctttagc cagcaacgaa atctgtcgat agaaagttgg
    15901 ttcgagagga acggaacaca attcacctgc aatgcttttc agcgttaact ctggttgtaa
    15961 ggcgatgcct aatccctgtc gaataaaatt catcgctgta tccgcctgat taaattcaca
    16021 ttttatctgc tgggcgcact attagtggta gtggctggtg gaatctatgc gctgcaaatg
    16081 aagggtaaaa gcggtgtcaa gctagtagtg gcaaaagaaa ttcattaata cgacacgttt
    16141 cattaagatt ttcctcaggt aaccacctat agtcattgtc gttaataaaa atgacaggcg
    16201 atagggtatg gcagtttcag caaagtacga cgaatttaat cactggtggg caacagaggg
    16261 cgactgggtt gaagagccta actatcgccg taatggtatg agcggggtgc aatgcgtcga
    16321 gcgcaacggc aaaaagctgt atgtaaagcg catgacgcat catctgtttc attccgtacg
    16381 ttatccgttc ggccgaccaa cgattgtccg tgaggttgcg gtaattaaag aactggaacg
    16441 ggccggtgtc atcgtgccga agatcgtttt tggtgaagcg gtgaaaattg agggtgaatg
    16501 gcgagcgttg ctggtgactg aagatatggc ggggttcatc agcattgctg actggtatgc
    16561 ccagcatgca gtatcgcctt attctgacga agtacggcaa gccatgttga aagcagtggc
    16621 gctggcgttt aagaaaatgc atagcattaa tcgtcagcat ggctgttgtt atgttcgcca
    16681 tatctatgtg aaaacagaag gtaatgcaga agctggtttt cttgacctgg agaaaagtcg
    16741 tcgccgcttg cgtcgcgata aagcgattaa tcatgacttc cgccagctgg aaaaatatct
    16801 ggagccaatc cccaaagcgg actgggagca ggtgaaagcg tattactacg ccatgtaatc
    16861 gttgtaggcc agataagacg cataagcgtt gtatctggca ctacaaccaa ttcacatctc
    16921 gatttcaata tcgccttttg cccggcaaca acagggcaaa atttcccccg gctgaataaa
    16981 ggctaacggt tcggcaatcc agtcaacctg acctgcaacc agacgggtgc gacaggagcc
    17041 gcagtaacct tcgcgacact gatactcaac cgccacattg tgggattcca gcgccgccag
    17101 aagggaaggg tgttcatcct ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg
    17161 ggccatcaga gttggaagtt actcaaatcg tcggtgtcca cttccgcgtc aatctgcccg
    17221 accagataag aactgacttc cacttcctgc ggagcaacct gcacgttatc agacaccagc
    17281 caggtgttga tccatgggat cgggttggag cgcgtctgga acggcagatc caaaccgact
    17341 gcctgcatac ggatattggt gatgtattca acgtactggc agagaatgtc tttattcaga
    17401 ccaatcatcg aaccgtcgcg gaacagataa tccgcccagt ctttctcctg ttgagctgcc
    17461 tgaacaaaca ggtcatagca ctcctgctta cactcttcgg caatttccgc catctcagga
    17521 tcgtccgcgc cgctgcgcag cagattcagc atatgctggg tgccggtcag gtgcagggct
    17581 tcgtcgcggg caatcaggcg aataattttg gcgttgcctt ccatcaattc gcgttctgca
    17641 aatgcgaagg aacaagcaaa gctgacgtag aaacgaatcg cttccagcgc gttaacgctc
    17701 atcaggcaga gatacagttt tttcttcagc tcgcgcaggc taacggtcac agttttaccg
    17761 ttaacggtgt gggtaccttc gcccagcaga tgccagtagc tggtcatttc gatcagctca
    17821 tcgtaatagc tggagatccc ttccgcacgt ttctggatct gctcgttggt gacgatatcg
    17881 tcaaacacaa cagacggatc gttaacgata ttacgaatga tatgagtata ggaacgggaa
    17941 tgaatcgttt ctgagaacgc ccaggtttcg acccaggtct ccagttccgg gatcgagatc
    18001 agcggcagca gtgccacgtt cgggctacgg ccctgaatgg aatccagcag cgtctgatat
    18061 ttcaggttgc tgataaagat gtgtttttcg tgttcaggca gtgcctggta atcgatacgg
    18121 tcgcgagaaa cgtcaacttc ttccggacgc cagaagaaag agagctgctt ttcgatcagc
    18181 ttttcgaaga tgtcatattt ttgctgatcg tagcgagcca cgttgaccgg ctgaccaaag
    18241 aacatcggtt ctttgagctg atcatttttc gtctgtgaaa aggtggtata tgccatgagt
    18301 gtgtcctgtt gggagtttaa tgccggataa ggcattttac gccgcatcct gtgcctgatg
    18361 cgacgctgac gcgtcttatc aggcctacaa atccgagccg taggccggat aaggcgttca
    18421 cgcagcatcc ggcacccgga gcctgatgcg acgctggcgc gtcttatcag gcctacaaat
    18481 ccgagccgta ggccggataa ggcgttcacg ctgcatccgg catctcaata tcagatctta
    18541 catgcgccgc tttcgcagcc atcgtcctgg attgacggta ccagatcgtc ctgcgcgtct
    18601 tcagcgccgt cacgggtgtt ctgataatac agcgttttga ccccgaattt gtaggcggtg
    18661 agcaggtctt tcagcaactg ctgcatcggc acttttcctg acgggaaacg tgacggatcg
    18721 tagttggtgt tagcagagat cgactgatcg ataaatttct gcatgatacc taccagttgc
    18781 aggtagccat cgttacccgg catttcccac agcagctcat aggcgtcgtg caggtgctcg
    18841 tagtccggca ccacctggcg caaaataccg tctttcgacg ctttgatgct gacgtaaccg
    18901 cgcggcggtt caataccgtt agtggcgtta gagatctgtg aagaagtctc tgacggcatc
    18961 agggcagaaa gcgtggagtt acgcaggccg tgcgttttga ttgattcacg caatgcttcc
    19021 cagtcgtaat gcagcggctc attagcgatg gtatccagat ctttcttata ggtatcgatc
    19081 ggcaggatcc ctttcgcgta agtggtttcg ttaaaccacg ggcacgcgcc ttgctctttt
    19141 gccagctcgt tagacgcttt cagcaggtaa tactgaatcg cttcgaaggt tttatgcgtc
    19201 aggttgttgg cgctgccgtc ggagtaacgc acaccgtgct tcgccaggta gtaagcgaag
    19261 ttgatcacac caatacccag cgtacgacga cccatcgctc cacgtttggc ggccgggatc
    19321 gggtaatcct gataatccag cagcgcgtca agtgcacgaa ccgccagaat tgccagctct
    19381 tccagttcat ccaggctatt aattgcgccc aggttgaaag cagacagcgt acacagcgcg
    19441 atttcaccgt tctcgtcgtt gacgtcgttc agcggtttgg tcggcagggc tatctccagg
    19501 cacaggttag actgacgcac tggcgcgatg gccggatcaa acgggctatg ggtattgcag
    19561 tggtcaacgt tctgaatata gatacgaccg gtagacgcac gttcctgcat catcagcgag
    19621 aacagctcaa cggctttcac acgctgcttg cggatgctgt cgtctttctc atatttggta
    19681 tacaggcgtt caaactcttc ctgatcggcg aagaatgcgt cgtacagccc cggtacgtcg
    19741 gacgggctga acagggtgat atcttcacct ttcagcaggc gggtatacat cagtttgttg
    19801 atctgtaccc cgtagtccat atggcgcacg cggttgcctt ccacaccacg gttgtttttc
    19861 aacaccagca ggctttccac ttccagatgc cacatcgggt agaacagcgt tgccgcaccg
    19921 ccgcgcacgc cgccctgaga gcaggatttc accgctgtct ggaaatgttt gtagaacgga
    19981 atacagccgg tatggaacgc ttcaccaccg cgaatcgggc tacccagcgc acgaatacgc
    20041 ccggcgttga tgccgatccc ggcacgctgg gaaacgtatt tcacaatcgc gctggaggta
    20101 gcgttgatgg aatccagact gtcaccgcac tcgatcagta cgcaggagct gaactgacga
    20161 gtcggggtac gcacgccgga catgattggc gtcggcagcg aaattttaaa tgtggaaacc
    20221 gcgtcgtaaa aacgcttcac atattgcagg cgcgtttcac gcgggtagtt cgagaacagg
    20281 cacgcggcaa ccagaatata aaggaactgg gcgctttcgt agatttcgcc ggtcacgcgg
    20341 ttctgtacca gatatttgcc ttccagctgc ttaacggcag cataagagaa ggtcatatca
    20401 cggtcgtgat cgataaacgt gtccatctgc ttgaactctt cttccgtgta gtcttccagc
    20461 agatgattat cgtatttgcc catctcgacc attttcacca cgtggtcgta cagcgcaggc
    20521 ggctcaaact ggccgtaggc ttttttacgc aggtggaaga tcgccaggcg cgcggcgaga
    20581 tactgataat ccggcgcatc acgggagatc aggtctgcgg ccgccttgat aatggtttcg
    20641 tggatgtcag aggtcttgat accgtcataa aactgaatgt gggagcgcag ctcgacctgg
    20701 gaaatcgaaa cgttatgcag tccttctgcc gcccaatcca gaacgcgatg gattttgtcg
    20761 agattgatgc gctctgtgct accgtcgcgc tttgtcacca gcagattctg attcatgtat
    20821 gtcgtacctg tttttggaat tctttccgca atagtttcat gcagactacc tgtagtacgg
    20881 gaagctgctt agaacactat atataggggg tatgtttggg attcactgca agatagtgtg
    20941 aaaatggccc tcttgcaagt gcataacttt gtggataact caggaaggaa aaagtggctt
    21001 tcgcgcacct taggtcagac aaggtgtccg ggaaagtcaa tgggaagaga aaaatttgtt
    21061 aaaaataact gttcggcgat ttcttgaacg gcagaatagg ttggataaat tgatatagat
    21121 ggtttattaa tctttttgct aatgattgca aaaattgatc taagcacgag tcatcacata
    21181 atctgatgta tcagttgtgg tcaatatata agcgaatgta agcgattgtt gatggcgaaa
    21241 tggttgtttt ctattcggtt attgttaaat gcgaagccat tttgttaatt taccttatat
    21301 tataagaaaa ttattcatta ctttaattta gtttaaatat aactggtatc ccttaaggtt
    21361 attaacttga attatgacgc actgatatta ttcatcaaat aataacaaaa tagccattgc
    21421 accgggttga acaatttaca aaagaaagat attccatatc gtataatgcg attaaatacg
    21481 ccgtcttata gaaaatgatg gttagattaa tatgcggatt atctttctac gcaaggagta
    21541 tttatcttta ctcccgtcaa tgattgcatc tcttttctct gctaacggtg tcgcggcggt
    21601 cactgattca tgccagggat atgatgtcaa agcgagttgt caggccagca ggcaaagcct
    21661 ttcaggcatt acgcaggact ggagtatcgc tgatgggcaa tggctggttt tttcggatat
    21721 gaccaataac gccagcggtg gggccgtatt tttgcaacaa ggagcggaat tttcactatt
    21781 accagaaaat gaaactggaa tgactctgtt tgccaataac accgttacag gagaatataa
    21841 taacggcggg gccatatttg ctaaagaaaa ctcaacgctg aatcttactg atgttatttt
    21901 ttccggtaac gtcgcaggcg gctatggtgg cgcaatctat tcttctggta ctaacgatac
    21961 tggtgccgtc gatttacgtg tcactaacac catgtttcgc aataacatcg ctaatgatgg
    22021 caaaggtggc gcaatttata ccattaataa tgacgtttat ttaagtgatg ttatttttga
    22081 taacaaccag gcatatacat caacaagtta cagtgatggc gatggcgggg caatcgatgt
    22141 taccgataat aatagcgaca gcaagcatcc ttcaggttat acgatagtaa ataacactgc
    22201 ctttacaaat aacactgccg aaggttatgg cggggcgata tataccaata gcgtgacggc
    22261 tccctatctt attgatattt ctgttgatga cagctacagc cagaacggag gcgtgttagt
    22321 cgatgagaac aatagcgcag caggctatgg agatggtcct tcctctgcgg cgggtggctt
    22381 tatgtatctc ggcataagtg aagttacctt tgatattgcc gacggaaaaa cgctggttat
    22441 tggcaataca gagaatgacg gagctgttga ctctattgct ggtaccgggt taatcaccaa
    22501 aacaggttcc ggcgatctgg tacttaatgc agataacaat gactttactg gtgagatgca
    22561 gattgaaaac ggtgaagtta ccctgggccg cagcaactcc ctgatgaatg tcggcgatac
    22621 gcattgccag gacgatccgc aagactgcta cggtctgacg atagggagta ttgatcagta
    22681 tcagaatcag gcagagctaa atgttggctc cacccaacaa acctttgtgc actcattaac
    22741 gggctttcag aatggcactt taaatatcga tgctggtggc aatgttactg ttaatcaagg
    22801 cagttttgct ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa acggcagcta
    22861 tgtgctggcg ggggcgcagt cgatggcgct aaccggcgat atagtcgttg atgatggtgc
    22921 ggtgcttacg ctggaaggcg acgcggcaga tcttgccgct ctccaggacg atccgcagtc
    22981 gatcgtgtta aacggcggtg tgctcgatct ctctgatttc tccacctggc agagtggcac
    23041 atcatacaac gatggccttg aagtcagcgg cagcagcgga acggttatcg gcagtcagga
    23101 tgtggtagat cttgcaggcg gaaacgatat gcatatcggc agcgacggga aaaatggcgt
    23161 ctacgtggtg gtcgatgcgg gggacgggca ggtcagcctg gcaaatgaca atcaatacct
    23221 cggcacaacg caaatcgcct ccggtacgct gatggtgagc gacaactcgc agcttggaga
    23281 tacccattat aaccgccagg ttatctttac cgataagcaa caagaaagcg tgatggagat
    23341 tactgccaat gtcgatactc gctctacaac gactgagcat gggcgtgata ttgaaatgcg
    23401 cgccgacggt gaagtggcag ttgatgcggg ggtagacacg cagtggggcg cactgatggc
    23461 tgacagcagc gggcagcatc aggatgaggg tagcacattg acgaaaacgg gggcgggtac
    23521 gctggagctg accgccagcg gtacaacgca gtcggcggta cgtgtcgaag aaggcaccct
    23581 gaaaggtgat gttgctgata tccttcctta tgcttcgtca ctgtgggttg atgatggggc
    23641 gacgttcgtt actggcgcgg atcaggatat tcagtcaatt gatgctactt ccagcggcac
    23701 tatcgacatc agcgatggta cggttttgcg cctgaccggg caggatactt ccgtcgccct
    23761 taatgcctca ctgtttaacg gcgatgggac gctggtgaat gctaccgatg gcgtgacgct
    23821 gacaggcgag cttaatacca atcttgaaac tgacagcctg acttatcttg ccgacgtgac
    23881 ggttaatggt gatctgacca atacgtccgg tgcggttagc ctgcaaaatg gcgtcgctgg
    23941 cgatacgctt acggtaaacg gtgattatac cggcggcggt acgctgctgc tcgatagcga
    24001 attaaacggc gatgactcgg taagcgatca attggtgatg aacggtaata ctgctggcaa
    24061 cacaactgtg gtggttaact ccattacagg gattggtgag ccgacatcta caggcattaa
    24121 agtggttgat ttcgcagctg atcccacgca gtttcaaaac aatgcgcagt tcagtctggc
    24181 aggcagtggc tacgtcaaca tgggagcgta tgactacacg ctggtggaag ataacaacga
    24241 ctggtatctg cgatcggaag cagtaacgcc gccatcgcca cctgatccag acccggaccc
    24301 aacccccgat cctgacccaa cacccgatcc aatccccgct taccagccgg tgctgaatgc
    24361 caaagttggc ggttatctca ataacctgcg ggcggcaaat caggcgttta tgatggagcg
    24421 acgcgatcac gctggtggcg atggtcagac gctgaattta cgtgttatcg gcggacgtta
    24481 tcattacacg gcggcagggc aactggctca gcaggaagac acttctacgg tgcagcttag
    24541 cggcgatctg tttagcgggc gctggggcac ggatggcgag tggatgcttg gggcggttgg
    24601 tggctacagt gataatcagg gcgacagccg ctcgaatatg accggaactc gcgccgataa
    24661 ccagaaccac ggttatgccg ttgggctgac ctcaagctgg tttcagcacg gtaatcagaa
    24721 gcagggggcc tggctggata gctggctgca atacgcgtgg tttaacaatg atgtttccga
    24781 acatgaagat ggcgtggatc gttaccattc gtcggggatt atcgcctcgc tggaagccgg
    24841 gtatcagtgg ttaccggggc gtggtgtggt gattgaaccg caggcgcagg tgatttatca
    24901 gggcgtgcag caggatgatt ttaccgccgc taaccgtgcg cgcgtgtcac aatcgcaggg
    24961 ggatgatatt cagacgcggc tgggtttaca cagcgaatgg cgtaccgcgg ttggtgtcac
    25021 accaacatta gatctgaatt attatcacga tccccatgcg acggaaattg aagaagatgg
    25081 cagcactatc agtgacgatg cggtgaagcg acggggtgaa ataaaagtgg gagtcacggg
    25141 caatatcagt cagcgagtgt ccctgcgcgg cagcgtggcg tggcagaaag ggagtgatga
    25201 ttttgcccag atggcagggt ttttgtcgat gacggtgaaa tggtaatcac tgtctagtcg
    25261 gcacggttgc cggatgcggc gtaaacgcct tatccggcct acggggagca tttgtaggcc
    25321 tgataagacg cggcaagcgt cgcatcaggc attggcgcgg ttgccggatg aatccaaaca
    25381 acaacctcac ttattctgcg tgtgcagcat atagttcaca tccacgccgg ggccgagttt
    25441 aaaagtatta gtgatcgggt tgtaatgcag cccagtgata tgccgctctt ttaaactggt
    25501 ctgatccacc cagcccagca attctgccgg tttaataaac ttcttcacat catgcgtgcc
    25561 tttgggcacc atgcgcaaaa tatattccgc gccaaccacc gccatcagcc atgacttgcc
    25621 gttgcggtta agcgtcgaga aaaagacatc gccgcctggt ttcaccagtt gcgcacaggc
    25681 tctgaccact gactgcggat cggggacgtg ctccagcatc tccatgcagg tcaccacatc
    25741 atactgcccg gcatgttttg ccgcgtgctc ttccacggtt tcctgcacat aatccacctg
    25801 aatgccgctt tccagcgcgt gtagttttgc cacctgcaat ggctcaaagc ccatatccag
    25861 accggtcacc gtcgcgcctt cgcgcgccat actttcggcc agaatgccgc cgccacaacc
    25921 gacatcgagc acctttttgc caaataaacc gccagcacgt tcggcaatat agcccagacg
    25981 cagcgggtta atgcggtgca gcggtttgaa ctcaccttca agatcccacc agcgggaggc
    26041 aacggcttca aatttagcga tctcttcgtg atctacgtta tggttttccg gcgatttttc
    26101 ggcattcatt ggcacttcta ctccgtaatt ggcaagacaa acgagtatat caggcattgg
    26161 atgtgaataa agcgtatagg tttacctcaa actgcgcggc tgtgttataa tttgcgacct
    26221 ttgaatccgg gatacagtag agggatagcg gttagatgag cgaccttgcg agagaaatta
    26281 caccggtcaa cattgaggaa gagctgaaga gctcctatct ggattatgcg atgtcggtca
    26341 ttgttggccg tgcgctgcca gatgtccgag atggcctgaa gccggtacac cgtcgcgtac
    26401 tttacgccat gaacgtacta ggcaatgact ggaacaaagc ctataaaaaa tctgcccgtg
    26461 tcgttggtga cgtaatcggt aaataccatc cccatggtga cttggcggtt tatgacacga
    26521 tcgtccgtat ggcgcagcca ttctcgctgc gttacatgct ggtagacggt cagggtaact
    26581 tcggttccat cgacggcgac tctgcggcgg caatgcgtta tacggaaatc cgtctggcga
    26641 aaattgccca tgaactgatg gccgatctcg aaaaagagac ggtcgatttc gttgataact
    26701 atgacggcac ggaaaaaatt cccgacgtca tgccaaccaa aattcctaac ctgctggtga
    26761 acggttcttc cggtatcgcc gtaggtatgg caaccaacat cccgccgcac aacctgacgg
    26821 aagtcatcaa cggttgtctg gcgtatatcg atgatgaaga catcagcatt gaagggctga
    26881 tggaacacat cccggggccg gacttcccga cggcggcaat cattaacggt cgtcgcggta
    26941 ttgaagaagc ttaccgtacc ggtcgcggca aggtgtatat ccgcgctcgc gcagaagtgg
    27001 aagttgacgc caaaaccggt cgtgaaacca ttatcgtcca cgaaattccg tatcaggtaa
    27061 acaaagcgcg cctgatcgag aagattgcgg aactggtaaa agaaaaacgc gtggaaggca
    27121 tcagcgcgct gcgtgacgag tctgacaaag acggtatgcg catcgtgatt gaagtgaaac
    27181 gcgatgcggt cggtgaagtt gtgctcaaca acctctactc ccagacccag ttgcaggttt
    27241 ctttcggtat caacatggtg gcattgcacc atggtcagcc gaagatcatg aacctgaaag
    27301 acatcatcgc ggcgtttgtt cgtcaccgcc gtgaagtggt gacccgtcgt actattttcg
    27361 aactgcgtaa agctcgcgat cgtgctcata tccttgaagc attagccgtg gcgctggcga
    27421 acatcgaccc gatcatcgaa ctgatccgtc atgcgccgac gcctgcagaa gcgaaaactg
    27481 cgctggttgc taatccgtgg cagctgggca acgttgccgc gatgctcgaa cgtgctggcg
    27541 acgatgctgc gcgtccggaa tggctggagc cagagttcgg cgtgcgtgat ggtctgtact
    27601 acctgaccga acagcaagct caggcgattc tggatctgcg tttgcagaaa ctgaccggcc
    27661 ttgagcacga aaaactgctc gacgaataca aagagctgct ggatcagatc gcggaactgt
    27721 tgcgtattct tggtagcgcc gatcgtttga tggaagtgat ccgtgaagag ctggagctgg
    27781 ttcgtgaaca gttcggtgac aaacgtcgta ctgaaatcac cgccaacagc gcagacatca
    27841 acctggaaga tctgatcact caggaagatg tggtagtgac gctctctcac cagggctacg
    27901 ttaagtatca gccgctttct gaatacgaag cgcagcgtcg tggcgggaaa ggtaaatctg
    27961 ccgcacgtat taaagaagaa gactttatcg accgactgct ggtggcgaac actcacgacc
    28021 atattctgtg cttctccagc cgtggtcgcg tctattcgat gaaagtttat cagttgccgg
    28081 aagccactcg tggcgcgcgc ggtcgtccga tcgtcaacct gctgccgcta gagcaggacg
    28141 aacgtatcac tgcgatcctg ccagtgaccg agtttgaaga aggcgtgaaa gtcttcatgg
    28201 cgaccgctaa cggtaccgtg aagaaaactg tcctcaccga gttcaaccgt ctgcgtaccg
    28261 ccggtaaagt ggcgatcaaa ctggttgacg gcgatgagct gatcggcgtt gacctgacca
    28321 gcggcgaaga cgaagtaatg ctgttctccg ctgaaggtaa agtggtgcgc tttaaagagt
    28381 cttctgtccg tgcgatgggc tgcaacacca ccggtgttcg cggtattcgc ttaggtgaag
    28441 gcgataaagt cgtctctctg atcgtgcctc gtggcgatgg cgcaatcctc accgcaacgc
    28501 aaaacggtta cggtaaacgt accgcagtgg cggaataccc aaccaagtcg cgtgcgacga
    28561 aaggggttat ctccatcaag gttaccgaac gtaacggttt agttgttggc gcggtacagg
    28621 tagatgactg cgaccagatc atgatgatca ccgatgccgg tacgctggta cgtactcgcg
    28681 tttcggaaat cagcatcgtg ggccgtaaca cccagggcgt gatcctcatc cgtactgcgg
    28741 aagatgaaaa cgtagtgggt ctgcaacgtg ttgctgaacc ggttgacgag gaagatctgg
    28801 ataccatcga cggcagtgcc gcggaagggg acgatgaaat cgctccggaa gtggacgttg
    28861 acgacgagcc agaagaagaa taattttact tcttcatgcc aaaagggagc tatctccctt
    28921 gtttgaattg aaaagtccag gctgcaaagt ctgggctttt gtcgtattag ggcacggtaa
    28981 agtttggctg tgcccgtaaa aaatggctgg ctatacacaa ggaatgtggc aatgagtggt
    29041 gaaaaaaagg cgaaaggctg gcggttctat ggtcttgtag gttttggcgc aatagcactg
    29101 ctttccgctg gcgtctgggc gttgcaatat gctggcagtg ggccagaaaa aacgttgtcg
    29161 ccgctggtgg tgcacaacaa tctgcaaatc gatctcaatg agccggacct ctttctcgac
    29221 agcgactctc tgagccagct tcccaaagat ctcctcacca ttccgtttct ccacgatgtt
    29281 ctgagcgaag atttcgtttt ctattatcag aatcatgccg atcgtctggg cattgaaggc
    29341 agcattcgtc gtattgtcta tgaacacgat ctcacgctga aagataagct cttttcgtca
    29401 ctcttagatc agcccgcgca ggcagcgctg tggcacgata aacaaggcca tctttcacat
    29461 tatatggtgc tgatccagcg cagtggttta agcaaactgc tggagccatt gttgtttgcc
    29521 gctaccagcg acagccagtt aagcaaaacg gaaatcagta gcatcaagat aaatagtgaa
    29581 actgttcctg tttatcagtt gcgctataac ggcaataacg ccctgatgtt cgcgacttat
    29641 caggacaaga tgctggtgtt ttccagcacg gatatgttgt ttaaagatga tcagcaggat
    29701 accgaagcca cggcgatcgc aggtgatttg ttgagcggca aaaaacgctg gcaagcaagc
    29761 tttggcctgg aagagcgtgc tgctgaaaaa acgccagtac gccagcgcat cgtggtcagc
    29821 gccaggttgc tgggttttgg ctaccagcgg ttaatgcctt cttttgctgg cgtacgcttc
    29881 gaaatgggta acgacggctg gcacagtttt gtggcgttaa atgatgaatc cgccagcgta
    29941 gataccagtt tcgattttac gccggtctgg aacagtatgc ctgccggggc cagcttctgt
    30001 gtggtggtgc cgtattcaca cggtattgcc gaagagatgc tttcgcacat cagtcaggaa
    30061 aacgacaagt tgaatggggc gttagacggt gccgcggggc tgtgctggta tgcagactca
    30121 aaattgcaaa ccccgctgtt tgtcggtcag tttgatggca ctgccgaaca ggcgcaattg
    30181 ccagggaaac tgtttacgca aaatattggt gcgcacgaaa gcaaagcgcc agaaggtgtt
    30241 ttgccgataa gccagactca gcagggcgaa gcgcaaatct ggcgtcgcga agtgagttcc
    30301 cgatacggtc agtatccgaa agcgcaggcg gcgcaacccg atcaattaat gtcggattat
    30361 tttttccgcg tgtcgctggc gatgcaaaac aaaacgctgc ttttctccct cgatgacacg
    30421 ctggttaata acgccctgca aacactgaat aaaaaccgcc cggcaatggt ggatgtaata
    30481 cccactgatg gcatcgttcc gctctatatc aatccacaag gcgtggcgaa actgctgcgt
    30541 aacgaaacgt tgaccagtct gccgaagaat ctcgaaccgg ttttttataa cgccgcacaa
    30601 actttattaa tgccgaagct ggacgcttta tctcaacaac cgcgttatgt catgaagctg
    30661 gcccagatgg aacccggtgc cgcctggcag tggctaccca taacctggca gccgctatga
    30721 ggcacgggct gctggcgctg atttgctggc tgtgttgtgt tgttgcccat agcgaaatgc
    30781 tgaatgtcga acaatccggg ctgtttcgcg cctggtttgt acgcattgca caagaacagc
    30841 tccgccaggg gccaagtcca cgctggtatc agcaggattg tgcgggcctg gtgcgatttg
    30901 cggcgaacga gacgctgaaa gttcacgata gtaaatggct aaaaagtaac ggtttctcta
    30961 gccagtattt gccgccagag atgacgctaa cacctggaca gcgtcaactg gcgcaaaact
    31021 ggaatcaggg gaacgggaaa accggcccct acgtgaccgc gattaatttg attcagtaca
    31081 acagccagtt tattggccag gacataaacc aggcgctgcc tggcgatatg atttttttcg
    31141 atcagggcga tgcccagcac ttaatggtct ggatggggcg ttacgtcatc taccacaccg
    31201 gaagcgccac gaaaactgac aacggaatgc gcgcagtcag tctgcaacaa cttatgacat
    31261 ggaaggacac ccgatggata cccaacgatt ccaatcccaa tttcattggc atttatcgtt
    31321 taaattttct ggcgcgatag cagcgtgctt gtccctgtct cttgtgggaa cagggcttgc
    31381 taatgctgat gattcgcttc cttccagtaa ctatgcgccg cccgccgggg gaacattctt
    31441 tttgcttgct gacagcagtt ttagcagcag tgaagaggcg aaagtgcgac tggaagcgcc
    31501 ggggcgtgat tatcggcgct atcagatgga agagtacggc ggcgtggacg ttcgcctgta
    31561 tcgtattcct gacccgatgg catttttgcg ccagcagaaa aacctgcatc gcattgtggt
    31621 gcaaccgcaa tatctgggcg acgggcagaa caatacgctg acctggctgt gggataactg
    31681 gtacggcaaa tctcgccgcg taatgcagcg tactttctct tctcagtcac ggcagaatgt
    31741 gactcaggca ttacccgaat tacatctcgg caatgccatt attaaacctt cccgttatgt
    31801 acagaacaac cagttttccc cgctgaaaaa atatcccttg gtggaacagt tccgttatcc
    31861 actatggcag gctaaaccgg tcgagccgca gcaaggggta aaactggaag gcgcatccag
    31921 caatttcatc tcgccgcagc cgggtaacat ttatattcct ctcggccaac aagagccggg
    31981 actgtacctc gtcgaggcga tggttggtgg gtatcgggcg acgacggtgg tgtttgtttc
    32041 cgataccgtg gcgcttagca aagtgtcagg caacgagctt ctggtgtgga ccgcgggtaa
    32101 aaaacagggt gaagcgaagc ccggctcaga gatcttgtgg actgacggtc ttggcgtgat
    32161 gacccgcggt gtgaccgatg acagcggtac cttgcagtta caacatatat cgccagaacg
    32221 ctcatacatt ctgggtaagg atgctgaagg cggcgttttt gtctccgaga acttcttcta
    32281 cgaaagcgaa atctacaaca cccgcttgta tatttttacc gatcgcccgc tatatcgcgc
    32341 aggcgatcgt gtcgatgtta aagtgatggg ccgcgagttc cacgatccgt tgcattcatc
    32401 ccccatcgtc agcgccccgg cgaagctttc ggtgctggac gctaacggca gtctgttgca
    32461 aaccgtcgat gtcacgctgg atgcgcgcaa tggcgggcag ggaagtttcc gcctgccaga
    32521 aaatgccgtt gccggaggtt atgagttacg tcttgcttac cgcaatcagg tctatagcag
    32581 cagttttcgc gtggcaaact acatcaagcc acatttcgag attggtttag ctctcgacaa
    32641 aaaagagttc aaaactggcg aagcggtcag cggcaaactg caactgctct acccggatgg
    32701 cgagccggta aaaaatgccc gcgtgcagtt aagtttgcgc gctcagcaat tatcaatggt
    32761 cggtaacgat ttgcgttatg ccggacgttt ccccgtgtcg ctggaaggca gcgaaacggt
    32821 gtccgacgcc agcggtcatg tggcgttaaa tctccccgcc gccgataaac cgagccgtta
    32881 tttgttaacc gtctccgcca gtgacggcgc ggcgtatcgt gtcaccacca ccaaagagat
    32941 cctcattgaa cgcggtctgg cgcattactc attaagtact gccgcacaat acagtaatag
    33001 cggcgagtcg gttgtgttcc gttatgccgc gctggaatct tcaaaacagg ttcctgttac
    33061 gtatgaatgg ttgcgtctcg aagaccgcac gagccatagc ggagagctac cgtcaggcgg
    33121 caaatccttt accgtcaatt tcgctaaacc tggcaactac aatctgacat tacgcgataa
    33181 agacggctta attctcgctg ggttaagtta tgccgtcagc ggtaagggca gcacggcgca
    33241 tacgggtacg gtagatatcg tggcggataa aacgctgtac cagccaggcg aaaccgcgaa
    33301 gatgctgatt acctttccgg agccaattga tgaagcatta ttgacgctgg aacgcgatcg
    33361 cgtggaacag cagtcgctgc tttcgcatcc ggcaaactgg ctaacgctac aacgtttaaa
    33421 cgatacccag tatgaagccc gggttccagt gagcaattcc ttttcgccta acatcacttt
    33481 ttcggtgctg tatacccgta atggtcagta cagttttcag aacgccggga tcaaagttgc
    33541 cgttcctcag cttgatatcc gggtgaaaac ggacaaaacc cattaccagc ctggtgaact
    33601 ggtcaatgtc gaattaacct cgtcgctgaa aggtaaacct gtttctgcgc agctaacggt
    33661 aggcgtggtc gatgaaatga tctacgcgct gcaaccagaa atcgcgccga atatcggcaa
    33721 atttttctat ccgctggggc gtaacaatgt gcgtaccagc tccagtctgt cgtttatcag
    33781 ctacgaccag gcgctctcca gcgagccggt tgcgcctggc gcaactaacc gcagcgagcg
    33841 gcgagtaaaa atgcttgaac gtccacggcg tgaagaggtg gataccgcgg catggatgcc
    33901 gtcactcaca accgataaac aaggcaaagc gtatttcacg ttcctgatgc ctgattcgtt
    33961 aacccgctgg cgtatcaccg cgcgtgggat gaacggcgac gggctggtcg ggcaggggcg
    34021 tgcttatctg cgttcggaaa aaaatctcta catgaagtgg agtatgccaa cggtgtatcg
    34081 cgtgggcgac aaaccggcgg caggactgtt tatcttcagt cagcaggata acgaaccggt
    34141 ggcgctggtg actaaatttg caggcgctga gatgcgccag acgctgacgc tgcacaaagg
    34201 ggcgaattat atttcgctgg cgcagaacat tcagcaatct ggcttgttaa gcgcagaact
    34261 gcaacaaaat gggcaagtgc aggacagcat tagcacaaaa ctgtcttttg tggataacag
    34321 ctggcccgtt gaacagcaga aaaatgtcat gctcggcggt ggcgagaacg cgctgatgtt
    34381 gcccgagcag gctagcaata tccggctaca aagtagcgaa acgccgcagg agattttccg
    34441 caacaatctt gatgcgttag tcgatggacc atggggtggc gtgatcaaca ccggtagccg
    34501 tctgatcccg ctcagtctcg cctggcgttc gcttgccgat catcaaagtg ccgccgctaa
    34561 cgacattcgt cagatgattc aggataaccg tctgcggctg atgcaactgg cggggcccgg
    34621 agcgcgcttt acctggtggg gtgaagatgg caatggtgac gccttcctta cggcatgggc
    34681 atggtacgcc gactggcagg ccagccaggc gctcggcgta acgcaacaac cggaatactg
    34741 gcagcatatg ctcgacagtt atgccgagca ggcggataac atgccgttat tgcatcgggc
    34801 gctggtgctg gcgtgggcac aggagatgaa tctgccgtgc aaaacgttgt tgaaagggtt
    34861 gaatgaagct atcgcccggc gcggaactaa agatgaagat ttctctgagg aagacatccg
    34921 cgatatcaat gacagcctga tcctcgatac accggaatct ccactggcag atgcggtggc
    34981 aaacgtctta accatgacgt tgctgaaaaa agcgcagttg aagtccacgg tgatgccaca
    35041 ggttcagcaa tatgcgtggg ataaagcggc aaacagcaat cagccgctgg cgcacacggt
    35101 tgtgctgctc aatagcggtg gtgacgctac ccagacggcc gctattttaa gtggtttgac
    35161 cgctgagcaa tccactattg agcgcgctgg ccatgaactg gctggcgaaa tatatggcga
    35221 caatgcctcc agttgttttg cctgcgcctg cgggcgcatg ggctaaacat aagttaactg
    35281 gagggggcga agactggcgt tgggttggtc agggtgtgcc ggacattctc tcttttggtg
    35341 acgaattatc gccgcaaaat gtgcaggtcc gctggcgtga accggcaaaa acggctcaac
    35401 aaagtaacat tccggtaacc gttgaacgcc agttgtatcg gcttatcact ggtgaagaag
    35461 agatgagctt tactctgcaa ccggtgacca gcaatgagat tgacagcgat gcgctgtatc
    35521 tcgatgaaat cacgcttacc agcgagcagg atgcagttct gcgctacggt caggtggaag
    35581 taccgctccc gccgggagcc gacgttgagc gcacaacatg gggcatttcg gtcaataaac
    35641 ccaacgccgc gaaacagcag gggcaattgc tggaaatagc gcgaaatgaa atgggcgaac
    35701 tggcctatat ggtgccggtg aaagaactga cgggaacggt cactttccgc catttgctgc
    35761 gcttctcgca aaaagggcaa ttcgttctgc ctcctgctcg ttatatgcgt tcctatgcac
    35821 ctgcacagca aagtgttgcg gcagggagcg aatggaccag gatgcaggtg aaataagtga
    35881 actggcgcag aatcgtctgg ctattggcgt tggttactct gccaacgctt gcggaggagc
    35941 cgccgttaca actggcgctg cggggcgcgc agcatgacca actgtataag ttatcgtcat
    36001 caggcgtgac aaatgtctcg acgttacctg acacgctcac cacaccgtta gggagcttat
    36061 ggaaactgta tgtttacgcc tggctggagg atacccatca accagaacaa ccataccagt
    36121 gtcgcggcaa ctccccggaa gaggtttatt gttgtcaggc tggggagagc ataacgcgcg
    36181 ataccgcgct ggtacgttcc tgtgggctct attttgcgcc gcagcggttg catattggcg
    36241 cagatgtgtg ggggcaatac tggcaacagc gtcaggcacc tgcgtggtta gcgtctctta
    36301 ctacgctgaa gccagaaacc tcggtaacgg ttaaatcgct gcttgattca ttagcgacgt
    36361 tacctgcgca aaacaaagct caggaagtct tactggatgt ggtgctggac gaggcgaaaa
    36421 tcggtgtcgc atccatgctc ggtagtcggg ttcgggtgaa aacctggagc tggtttgccg
    36481 atgataaaca ggaaatccgt cagggtggtt ttgctggctg gttaaccgat ggtacaccgc
    36541 tgtgggttac tggcagtggc accagcaaaa cggtactaac ccgttatgcg acggtgctta
    36601 atcgggtttt gccagtgccg acgcaggttg ccagtgggca atgtgtagag gtggagctgt
    36661 ttgcccgcta tccgctgaag aaaatcacag cggaaaagag tacgacggca gtaaaacccg
    36721 gcgtacttaa tgggcgctat cgtgtgacgt ttactaatgg taaccacatt acgtttgtca
    36781 gccacggtga gacgacctta ctgagcgaaa agggcaaact gaaattgcag tcacatctcg
    36841 atcgtgaaga gtacgttgcc agagttctgg atcgcgaggc gaaaagtacg ccccctgagg
    36901 cagcgaaagc catgaccgtg gcgattcgca cttttttaca gcaaaacgcc aatcgggaag
    36961 gcgattgcct gaccatccct gacagcagcg ccacgcagcg tgtttcggct tcgccagcta
    37021 cgacgggggc gcgaacgatg gccgcctgga cgcaggatct catctatgcg ggcgatccgg
    37081 ttcattatca tggcagtcgc gctaccgaag ggacactttc ctggcggcaa gccacggcct
    37141 aggcggggca gggagagcgt tacgaccaga tactcgcgtt tgcttatccc gacaacatcc
    37201 ttagccgctg gggagcgcca cgctcaacct gccagttatt acccaaagcg aaagcctggc
    37261 tggcgaaaaa aatgccgcag tggcggcgta tattacaagg cgagacgggg tacaacgaac
    37321 cagacgtgtt tgcggtctgt cgtttagtct caggtttccc ctataccgat cggcagcaga
    37381 aacggctgtt tattcgtaac ttctttacgc ttcaggatcg gctggattta acccatgagt
    37441 atctgcacct ggcctttgat ggttatccca ccgggcttga tgagaactat atcgaaacgc
    37501 tgacccgaca attattaatg gactaatgct atgcgaaaga tttttcttcc gttgttactg
    37561 gtggcacttt cgcctgtcgc tcacagtgaa ggtgtgcagg aagtcgagat tgacgcaccg
    37621 ctctctggct ggcatccggc agagggcgaa gatgccagtt tttcacaatc gatcaattac
    37681 ccggcgtcgt ccgtcaacat ggcagatgat caaaatatct cagcgcagat ccgcggtaaa
    37741 ataaagaatt atgctgcggc gggtaaggtt cagcagggcc ggctggtggt caacggtgcc
    37801 agtatgccgc agagaattga atccgatggt tcatttgcac gcccttatat tttcactgaa
    37861 ggcagcaaca gtgtgcaggt catcagcccg gatgggcaaa gccgacaaaa aatgcagttt
    37921 tactcaacgc cgggtgctgg ggcgattcgt gcacgtttac ggctggttct ctcgtgggat
    37981 acggacaata ccgatctcga tctccatgtt gtcacgcccg atggcgaaca cgcctggtac
    38041 ggtaacaccg tgctgaaaaa cagtggtgca ctggatatgg atgtcacgac ggggtacgga
    38101 cccgagattt tcgccatgcc agcgccagtt cacggacgct atcaggtgta tatcaactac
    38161 tatggtgggc gcagcgaaac ggaattaacc accgcccaac tgacgctgat taccgatgaa
    38221 ggatcggtca atgagaaaca ggaaacattt attgtaccga tgcgtaatgc tggcgagctg
    38281 acgctggtga aaagttttga ctggtgatta atggggttac cttagccgtc gatgttcaag
    38341 aaaacacccg ataactttcg ctatcgggtg tttttattga ttaattcaac cgttcaatca
    38401 ccatcgcaat tccctggccg ccaccaatgc acagtgttgc cagccccagc gttttatcgc
    38461 gtgcctgcat ggcgtgtaat agtgtgacca gaatacgagc accactggca ccgataggat
    38521 gcccgagcgc gatggccccg ccgttgacat tcactttctc agaatcaaag cccagggttt
    38581 tcccaacggc aaggaactgt gcagcaaatg cttcattagc ctcaatgaga tcaatatccg
    38641 ccagttgcag ccccgccagt tgtaacgctt tttgcgtggc aggtactggc cccataccca
    38701 tcaatgcggg gggcacgcca ccgctggcat aacttttaat gcgagccagg ggggtaaggc
    38761 ctgctgccag cgccgcagat tcttccataa tcaccagagc ggcagcaccg tcgttaatac
    38821 cagacgcgtt cccggcggtg actgttcctg ctttatcgaa ggccgggcgc aatgcgccta
    38881 acgcttcagt cgtagaatcc gctttcggga attcgtcttg actgaagacg aaggttttct
    38941 tccgagtgac aacatttacc gggacgattt cggctgtaaa agcaccggac tcaattgcgg
    39001 ctgccgcttt acgctgtgaa tgtagcgcca gttcatcctg catatctcgg gtaattccgt
    39061 actctttggc cacgttctcg gcggtaatcc ccatatgata accatgggtg gcgcacatca
    39121 gaccatcgcg caggattacg tcataaacct gtccgtctcc aagacgataa ccagagcgtg
    39181 cttttgcatc gagtaagtag ggcgctaaac tcatattttc catacccccc gccacaatgc
    39241 tttgcgcctg acctgcctga attgcctggg cggcaagcgc cacactttta agacccgaac
    39301 cacacacttt attgaccgtg aatccgcaca ccgtttctgc cagcccgctt tttaacagtg
    39361 cctgacgtgc cggattttgc cccaacccgg cttgcaacac gttacccata atcacttcat
    39421 caatgtgtag tgaatcgatt tttgcacgtt caatggcggc tttaattact gtcgccccca
    39481 ggtcgatggc gctggtggaa gcgagtgaac cgttaaaact accgatagca gtacgtaccg
    39541 cactgacgat gacacaattt ttcatttttt attccttctg tctgtagggt tgccgtcaga
    39601 acagcgttaa accaatgacg aaaatgacgc cggagaagag cagggcagtg atgcaatagc
    39661 ccatgatgtc gcggacaccg agtccggcga ttgccagtgc tggcagcgcc cagaatggtt
    39721 gtgccatgtt catccattgc tcgccgtagg cgatcgccat taccgattta ccgagatcag
    39781 cgcccagcgc ctgggctgcg ggtatcacga aaggtccctg aataacccag tgaccgccgc
    39841 cagacggaac ggcgaagtta atcagtgcag aactaaaaaa ggtcattacc gggaaggtgt
    39901 ctttgttcgc aacattgatg aagaattcgg taatgagtcc gcccagaccg gaatgctcca
    39961 tcatcagttg gatcccagcg tagaagggga attgcaccag aataccggca gtactgcgtg
    40021 ctgccgcgct gatagcacgc atataagcca ttggcgtttt atgtagcagc agacccgcaa
    40081 tcataaacat caggttgacg gtattgatgg tgatgttgaa gccatgttcg ctgaagtaca
    40141 tcgcaaggta agcgataccg agtgcgccga tgatcaacgt cagaatgcgg ctttcttcca
    40201 gtcgctcgga tggtggggca tctttcggta gctgcttttg aaaatcagcc tcttccatga
    40261 gtagtttcgg atcgatactc accacgtcag acggttttgg catcatcatg cgggtgataa
    40321 atggcatcac cacaatcaac gccacagtga tgaaaatatt aaaaccactg aacagagtat
    40381 cgcccaccgg gatcagcccg gcgatatgct caaccgggtt gcccggtgtt gcagccaaca
    40441 gaggcattga gccagagaag ccgccacccc aggtgagaaa accaatgtag gcgcaggcaa
    40501 tgagcaacgg ataatcagaa ccgggtactc ggcgggcgac ttcacgggca aacattgcgc
    40561 cgacaaccaa accaaatccc cagttgatga cacaagcgac tgaaccgaag aaagtgacca
    40621 gcatgacgcc ctgtacgggc gtctttgcgg cggaggcggc agtacgcagc aaacttttta
    40681 ccggagcgga gctggcaagg gcatgaccgg taacgatgat aagcgccatc tgcataccaa
    40741 acgccagcaa gttccagaaa ccgtcacccc acattttcac catgctgatc ggcgtttgtg
    40801 gtgttaacca aagcgcgatc acgaatgtta gcaatgtcag caacatggca aagatcagtg
    40861 gatcgggaag ccaccggctg acaaaacgcg tcataaaacg cgatatgcga ccaatcataa
    40921 atcaccccgt tgcgtattca gatcggcggc gacttcaaac cgagcttctg ttttggcacg
    40981 cacggtggct aaatcacacc cgtcggcaat ttcggtgagc cacattttgc cgtcaataaa
    41041 acgaaagaca gccagttcag taaccagcat atgcaccgca tgttgcgcag tgagtggcat
    41101 ggtgcagcgg cgcaaaattt ttgctgaacc atctttggcg caatgttcca tggcgatgat
    41161 cactttgcgc gacccggtca ccagatccat cgcgccaccc ataccgggca ccattttccc
    41221 aggcactacc cagttcgcga ggtttgcttc ttcgtctact tgcaaaccgc cgagcacgca
    41281 ggcatcaata tgaccgccac ggattagcgc aaatgacatg gcgctatcaa acatggctgc
    41341 accgggtaaa acaccgcacg gttgcccgcc agcgttcacc agatctggat gcgctgtcgt
    41401 gaccgggcct aaaccgagga agccgttttc cgattgcaga gtgatatgaa taccctccgg
    41461 taaataattg gcgaccattg tgggtaaacc gatccctaag ttaacgatgt caccatcacg
    41521 aagctcttgc gccacacggc gcgcaatacg ttgtttcgca tccattattt gctctcctgt
    41581 gaaacgatga tgtggtcgat aacggcacca ggggtgacaa tatggtcagg ttgcagctcg
    41641 ccggtttcga ccagttcatc tggctctacc agcgtgatat cagccgcaag ggctatcagg
    41701 gggttaaagt tgcgggcgct aagttgatag gtcaggttgc caagtgtgtc gcaacgatga
    41761 gcgcgaatta gcgccaggtc ggcgcgcagt gggcgttcga gcagccaggt tttaccgtcg
    41821 agtgtcagtg tctgtttgcc ttcctctacg acggtgccga cacccgttgg ggtgagaaaa
    41881 ccaccaagtc cagctccacc acagcgaatt tgctcgatta gcgtaccttg cggcaccaga
    41941 acgacgtcca tctcaccaga tatcatgcgc cgacctgttt ccgggttggt gccgatatgt
    42001 gaagcaatca ctttgcggac tcgaccattg acgatgagcg gaccgatgcc ggtatcaaca
    42061 aacgcggtat cattggctat caatgtcagg tcgcgaacac cagattccag taatgcttca
    42121 accaggcggg atggagtgcc aatccccata aatccgccca ccatgatggt catgccgtca
    42181 cgaaagaagc cggtggcgtc ttgtaatgtc atcaattttg ttttcatttt tatccctctt
    42241 gcatacaccg ttgtgggtac agtcaggcaa tagcaagggg agtgccagaa tgagattatt
    42301 ggatttagtt atttaactaa ttgaatatga agggataaat tggatgggga tttataagga
    42361 gaatgagcgg ctatgcagaa aattgcgcac tgtgcaaatt tctgcatagc aagttttggt
    42421 gttatacatc cgccggatcg ataccgtatt cctggagttt atacatcaat gcacggcgac
    42481 tgatgcccag cattaaagca gtgcgggttc ggtttccttc ttgttgttcc agcacttcca
    42541 taatgatgcg tttttcgacg cgtttaattt cctcttttaa attacgctca ccgacagggg
    42601 ctgtttttac ctcgccagca ttacagactg gctgacgaat ctgtggcgga agatcctcag
    42661 aaaaaatgat cgggcctgaa ttcatcacga cggcgcgttc aataacgttg gaaagctctc
    42721 gaatatttcc cggccatgac caggcggtaa gcagtgacat tgccatcgga tcgatgtcga
    42781 taatatcgcg ctgattctca ctactgaatt tttgcaaaaa gtgattagct aacagggaaa
    42841 tatcttcccg gcgatcgcgc agaggcggca gtattaaatg aataacgtta aggcgataaa
    42901 agagatcttc acggaaggtg ccttctttta ccattgcctg caagtcacgg ttggtggcac
    42961 caatgatgcg gatatcaact tttatggtct gatggccgcc aatccgttca aattcccgtt
    43021 cctgtagaat gcgtagtaat ttggcttgta gtaccagcgg catttcgcca atttcgtcga
    43081 ggagcagagt accttcgttg gctcgttcaa ataatccctg acgcaaggtt tgtgcaccag
    43141 taaatgcacc tttttcatga ccaaacagtt cactttcgag caacgattcc ggcagcgccg
    43201 cgcagttgac tttaatgaac ggcccctttg cccgccgcga attgtagtga atcgctctgg
    43261 caatcaactc tttcccggtg ccgctttcac cgctaatcaa gacgctggcc tgagaaaggg
    43321 caattttggc ggtgtttttg cagatgtcca tcatcgccgg gctgttggtg agaatgtgcc
    43381 cccattgcca gctggtgctc agtgcctggt gcagatgacg gatctctttt ttcattgact
    43441 ggagttgtaa agcgcgctga acgattaaat tcaactcatc gagatcaaac ggtttaataa
    43501 catagtcgaa ggctccgcag cgtagcgctt cgacggcggt ttccacttcc gcataggccg
    43561 tcatcagaat aacgggtgtc cgggtctcat ggctgcgcat ctcctttagt gccttgatgc
    43621 cgtccatctc tggcatgcgg atatccatca acaccacatc agggtgaata tcggcaaaca
    43681 ggtgtaatgc tgtgcgtccg ttgttcgcac aatttgtttc gaatccttgt agtgcaaaag
    43741 cggtgctcag catacggcga acattatctt catcatccac aataaggatg cgattaatag
    43801 cagtcataca gtctgatttc cctgcgggtt gatcggtaaa ataagcgtga aggttgcgcc
    43861 gtagcccggc aaactggcga cgcgaatatc accctgatgg gcattaatga tgcgttgact
    43921 taacgccaga ccaagcccgg ttcctgaggc tttggtggtg aaaaagggat cgaagatctt
    43981 tttttgcagc gagagatcaa tgccacagcc gttgtcctct atcgaaatgg cctgttgtga
    44041 gtcgctgtat tgccaggttt gaatgcgaat tttccctcgt gcgctgatag cctggacggc
    44101 attgatcagg atattcagta gtacctgttt gagcagttca cgatcggcgt taatcgggct
    44161 taattcatta tccagttcgc ttatgaagtc gacccgcgct tgtacgccgg cggtttgtac
    44221 cagtaccaga gtttcttcaa ccaatgcatt gaggctgact tgttgccatt gactgtggcg
    44281 tggacgtgaa aattcgagca attgctgaat aactttgtta attgaatcga tttctttgag
    44341 tactacggac agatattcct gatgtattgg gtcactggtt tgttggcgca agatctgtac
    44401 ataaccacga atagccgtta acggattacg tacttcatgc gcgacgccag ccatcagctc
    44461 acccagtgtg gcgaggcgtt ctgcttgcgc catgcggcgc tgggtttctt tgcgggcagt
    44521 taaatcagag aaaatcacca aagcacctat catttcaccg tgcgtgttat gaatacgact
    44581 ggtagtgaca ctgagttcaa tggtgcggtc acgacctgga aaactgatct ccagcgccac
    44641 atgttcggtg ccatgttcca gcgtatccag tactggactg tagaactgag tattgtcgaa
    44701 caacatggag taaggctgcc ctaccagttc atggcgttga tagccagtga taacttctgc
    44761 tgctgggttc atggtggtta catcaccctg gcggtcaatg gcaatgacgc catcggcagc
    44821 gttttcaata atcagatcgt taagtgtccg cgtttcacgc agtgcctggg cgaggttatt
    44881 aacactctga ctgatttgcc ccatttcacc gggcaattgt ggtaatcgag tgggaatatt
    44941 ttgtgccaga gtcgagaggc catcggtgat gatatcaata ttggcgctca ggcgacggga
    45001 gaaaaggaca atcaatagca ggcttatcag caaaccggcg gtgagcacaa tgataatcct
    45061 cacatccatt ttccaggcct ggcggcgaat atcttcggtt aattcattgg cccagatata
    45121 gccgaggatt tcaccattac gctcaatggg gagcattgaa ttcaaaatat cgccgcgcac
    45181 ctgcctgcct gaataaacca aaggggtatt tgtacgcatg acttcgcgac cagggtgatc
    45241 tgcggcaatg gtaacgccga cattattctg
//
LOCUS       DAOFQD010000024        44904 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.024, whole genome shotgun sequence.
ACCESSION   DAOFQD010000024 DAOFQD010000000
VERSION     DAOFQD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.024"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            35..661
                     /gene="upp"
                     /locus_tag="QBF46_001402"
     CDS             35..661
                     /gene="upp"
                     /locus_tag="QBF46_001402"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDQ6673601.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            747..2036
                     /gene="uraA"
                     /locus_tag="QBF46_001403"
     CDS             747..2036
                     /gene="uraA"
                     /locus_tag="QBF46_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDQ6673602.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            2086..2832
                     /gene="hda"
                     /locus_tag="QBF46_001404"
     CDS             2086..2832
                     /gene="hda"
                     /locus_tag="QBF46_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDQ6673603.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(2970..3329)
                     /gene="arsC"
                     /locus_tag="QBF46_001405"
     CDS             complement(2970..3329)
                     /gene="arsC"
                     /locus_tag="QBF46_001405"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDQ6673604.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3350..4813)
                     /gene="bepA"
                     /locus_tag="QBF46_001406"
     CDS             complement(3350..4813)
                     /gene="bepA"
                     /locus_tag="QBF46_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDQ6673605.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            5026..6087
                     /locus_tag="QBF46_001407"
     CDS             5026..6087
                     /locus_tag="QBF46_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDQ6673606.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(6125..6973)
                     /locus_tag="QBF46_001408"
     CDS             complement(6125..6973)
                     /locus_tag="QBF46_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDQ6673607.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLICFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(6995..9007)
                     /gene="hyfR"
                     /locus_tag="QBF46_001409"
     CDS             complement(6995..9007)
                     /gene="hyfR"
                     /locus_tag="QBF46_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDQ6673608.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLTRIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRCMPGESVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGDTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEERQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLRMKRTTLLSRMQRLGISVREVL"
     gene            complement(9037..9450)
                     /gene="hyfJ"
                     /locus_tag="QBF46_001410"
     CDS             complement(9037..9450)
                     /gene="hyfJ"
                     /locus_tag="QBF46_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDQ6673609.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(9443..10200)
                     /gene="hyfI"
                     /locus_tag="QBF46_001411"
                     /pseudo
     CDS             complement(9443..10200)
                     /gene="hyfI"
                     /locus_tag="QBF46_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(10197..10742)
                     /gene="hyfH"
                     /locus_tag="QBF46_001412"
     CDS             complement(10197..10742)
                     /gene="hyfH"
                     /locus_tag="QBF46_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDQ6673610.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(10752..12467)
                     /gene="hyfG"
                     /locus_tag="QBF46_001413"
     CDS             complement(10752..12467)
                     /gene="hyfG"
                     /locus_tag="QBF46_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDQ6673611.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPILFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGDSDARVIPVGPLHITSDEPGHFRLFVDGEHIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRMMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTMPYKEIERYGIDRN
                     RSPLK"
     gene            complement(12457..14037)
                     /gene="hyfF"
                     /locus_tag="QBF46_001414"
     CDS             complement(12457..14037)
                     /gene="hyfF"
                     /locus_tag="QBF46_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDQ6673612.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRRLSATRTVTVLHSLGIT
                     LLLILALRVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGIYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(14042..14692)
                     /gene="hyfE"
                     /locus_tag="QBF46_001415"
     CDS             complement(14042..14692)
                     /gene="hyfE"
                     /locus_tag="QBF46_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDQ6673613.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHDTLDVNNLTALKG"
     gene            complement(14704..16143)
                     /gene="hyfD"
                     /locus_tag="QBF46_001416"
     CDS             complement(14704..16143)
                     /gene="hyfD"
                     /locus_tag="QBF46_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HDQ6673614.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(16160..17107)
                     /gene="hyfC"
                     /locus_tag="QBF46_001417"
     CDS             complement(16160..17107)
                     /gene="hyfC"
                     /locus_tag="QBF46_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDQ6673615.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQEVQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(17118..19135)
                     /gene="hyfB"
                     /locus_tag="QBF46_001418"
                     /pseudo
     CDS             complement(17118..19135)
                     /gene="hyfB"
                     /locus_tag="QBF46_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(19135..19752)
                     /gene="hyfA"
                     /locus_tag="QBF46_001419"
     CDS             complement(19135..19752)
                     /gene="hyfA"
                     /locus_tag="QBF46_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDQ6673616.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFRAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(20005..20475)
                     /gene="bcp"
                     /locus_tag="QBF46_001420"
     CDS             complement(20005..20475)
                     /gene="bcp"
                     /locus_tag="QBF46_001420"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDQ6673617.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(20475..21047)
                     /locus_tag="QBF46_001421"
     CDS             complement(20475..21047)
                     /locus_tag="QBF46_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDQ6673618.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            21193..22071
                     /gene="dapA"
                     /locus_tag="QBF46_001422"
     CDS             21193..22071
                     /gene="dapA"
                     /locus_tag="QBF46_001422"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDQ6673619.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            22088..23122
                     /gene="bamC"
                     /locus_tag="QBF46_001423"
     CDS             22088..23122
                     /gene="bamC"
                     /locus_tag="QBF46_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDQ6673620.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VSVFQAAFSK"
     gene            23335..24048
                     /gene="purC"
                     /locus_tag="QBF46_001424"
     CDS             23335..24048
                     /gene="purC"
                     /locus_tag="QBF46_001424"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDQ6673621.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            24216..25079
                     /locus_tag="QBF46_001425"
     CDS             24216..25079
                     /locus_tag="QBF46_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDQ6673622.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            25094..27109
                     /gene="tmcA"
                     /locus_tag="QBF46_001426"
     CDS             25094..27109
                     /gene="tmcA"
                     /locus_tag="QBF46_001426"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDQ6673623.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHVLKLRDALP
                     GDWLWVSPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQQEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREETAQALFALNDVRTERLRDRITQWQFFH"
     gene            27183..27881
                     /gene="ypfH"
                     /locus_tag="QBF46_001427"
     CDS             27183..27881
                     /gene="ypfH"
                     /locus_tag="QBF46_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDQ6673624.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(27991..28191)
                     /locus_tag="QBF46_001428"
     CDS             complement(27991..28191)
                     /locus_tag="QBF46_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDQ6673625.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(28219..29346)
                     /gene="dapE"
                     /locus_tag="QBF46_001429"
     CDS             complement(28219..29346)
                     /gene="dapE"
                     /locus_tag="QBF46_001429"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDQ6673626.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(29350..29706)
                     /locus_tag="QBF46_001430"
     CDS             complement(29350..29706)
                     /locus_tag="QBF46_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDQ6673627.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            30087..30146
                     /gene="ypfM"
                     /locus_tag="QBF46_001431"
     CDS             30087..30146
                     /gene="ypfM"
                     /locus_tag="QBF46_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDQ6673628.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(30245..33358)
                     /gene="acrD"
                     /locus_tag="QBF46_001432"
     CDS             complement(30245..33358)
                     /gene="acrD"
                     /locus_tag="QBF46_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDQ6673629.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGNIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELPRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LITSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(33522..35222)
                     /gene="narQ"
                     /locus_tag="QBF46_001433"
     CDS             complement(33522..35222)
                     /gene="narQ"
                     /locus_tag="QBF46_001433"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDQ6673630.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            35428..37407
                     /gene="aegA"
                     /locus_tag="QBF46_001434"
     CDS             35428..37407
                     /gene="aegA"
                     /locus_tag="QBF46_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDQ6673631.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEIPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            37475..38050
                     /gene="nudK"
                     /locus_tag="QBF46_001435"
     CDS             37475..38050
                     /gene="nudK"
                     /locus_tag="QBF46_001435"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDQ6673632.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            38176..39219
                     /locus_tag="QBF46_001436"
     CDS             38176..39219
                     /locus_tag="QBF46_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDQ6673633.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGDQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(39314..41317)
                     /gene="tkt"
                     /locus_tag="QBF46_001437"
     CDS             complement(39314..41317)
                     /gene="tkt"
                     /locus_tag="QBF46_001437"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDQ6673634.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(41337..42287)
                     /gene="tal"
                     /locus_tag="QBF46_001438"
     CDS             complement(41337..42287)
                     /gene="tal"
                     /locus_tag="QBF46_001438"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDQ6673635.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            42576..44855
                     /gene="maeB"
                     /locus_tag="QBF46_001439"
     CDS             42576..44855
                     /gene="maeB"
                     /locus_tag="QBF46_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDQ6673636.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT        10837 a        11717 c        12024 g        10326 t
ORIGIN      
        1 tttttttgtg gctgcccctc aaaggagaaa gagtatgaag atcgtggaag tcaaacaccc
       61 actcgtcaaa cacaagctgg gactgatgcg tgagcaagat atcagcacca agcgctttcg
      121 cgaactcgct tccgaagtgg gtagcctgct gacttacgaa gcgaccgccg acctcgaaac
      181 ggaaaaagta actattgaag gctggaacgg cccggtagaa atcgaccaga tcaaaggtaa
      241 gaaaattacc gttgtgccaa ttctgcgtgc cggtctgggg atgatggacg gtgtgctgga
      301 aaatgttccg agcgcgcgca tcagcgttgt cggtatgtac cgtaatgaag aaacgctgga
      361 gccggtaccg tacttccaga aactggtttc taacatcgat gagcgtatgg cgctgatcgt
      421 tgacccaatg ctggcaaccg gtggttccgt tatcgcgacc atcgacctgc tgaaaaaagc
      481 gggctgcagc agcatcaaag ttctggtgct ggtagctgca ccagaaggta tcgctgcgct
      541 ggaaaaagcg catccggacg tcgaactgta tactgcatcg attgaccagg gactgaacga
      601 gcacggatac attattccgg gcctcggcga tgccggtgac aaaatctttg gtacgaaata
      661 aagaataaaa ataattaaag ccgactttaa gagtcggctt ttttttgagt aaagcgccta
      721 taacacataa tacagaggat aatactatga cgcgccgtgc tatcggggtg agtgaaagac
      781 cgccactttt acagacaatc ccgcttagtt tgcaacattt gttcgccatg tttggtgcaa
      841 ccgtcctggt gcccgtctta tttcatatta acccggcgac tgtactgtta tttaacggta
      901 ttggaacgct gctgtatctc ttcatctgta aagggaaaat tccggcttat cttggttcca
      961 gctttgcctt tatttcaccg gtattgctac tgttgccgtt ggggtatgaa gtcgcgctgg
     1021 gcggctttat tatgtgcggc gtgctgttct gccttgtttc ttttatcgtg aagaaagcgg
     1081 ggaccggctg gctggacgtg ctgtttccac ctgcggcaat gggcgcaatc gttgccgtca
     1141 tcggtctgga gctggcgggc gtagctgccg gtatggcggg tttactcccg gctgaagggc
     1201 aaacgccaga ctccaaaacc atcatcatct ctattaccac cctggcggtc acggttttag
     1261 gttccgtgct gtttcgtggt ttcctggcaa ttatcccgat tttaattggc gtgctggtgg
     1321 ggtacgcgct ctctttcgca atgggaattg tcgataccac gccgattatt aatgctcact
     1381 ggtttgcgct gccaaccctc tatacgccgc gcttcgagtg gtttgccatt ctgactattc
     1441 tgccagcggc attagtggtt attgccgaac acgtagggca cctggtagta acggctaata
     1501 tcgtcaaaaa agatctgctg cgcgatccag gtctgcaccg ttcgatgttt gctaacggcc
     1561 tgtcgaccgt gatctccggc ttctttggct ctacgccaaa taccacttac ggagaaaaca
     1621 ttggcgtgat ggcgatcacc cgtgtttaca gtacttgggt tatcggcggg gctgcgattt
     1681 tcgctatcct gctttcctgc gtcggtaaac tggctgccgc tatccagatg atcccattgc
     1741 cggtgatggg cggcgtttcg ctactgcttt atggtgtcat cggtgcttcc ggtattcgtg
     1801 ttttgatcga atcgaaagtg gattacaaca aagcacagaa cctgatcctg acttccgtga
     1861 ttttgatcat cggcgtcagt ggcgcgaagg taaacatcgg cgcggcggag ttgaaaggta
     1921 tggcgctggc gaccatcgtc ggtatcggct taagtttgat cttcaaactg atcagtgtgc
     1981 tgcgccctga agaagtggtg ctggacgcag aagacgcgga tataacagac aaataatgta
     2041 ccgaccgggc agtgttgctg cccggttcaa acatcatggg attctgtggt aaacttctcg
     2101 cgattttgtg aaatcctggt tgaggtatct ctgaacacac cggcacagct ctctttgcca
     2161 ctttatcttc ctgacgacga aacctttgca agtttctggc cgggggataa ctcctcttta
     2221 ctggccgcgc tgcaaaacgt gctgcgtcag gaacatagcg gttacatcta tctctgggca
     2281 cgcgaaggcg cggggcgcag tcatctgctg cacgcggctt gcgcggaatt gtcgcagcgt
     2341 ggcgatgcgg tgggctatgt cccgctggat aaacgcacct ggtttgttcc ggaagtgctc
     2401 gacggtatgg agcatttgtc gctggtctgt atcgacaaca ttgagtgtat tgcaggcgat
     2461 gagttgtggg agatggcgat tttcgatctc tacaatcgaa ttctggaatc gggcaaaaca
     2521 cgattgttga tcaccggcga tcgtccaccg cggcagttga atctgggatt accggatctc
     2581 gcttcgcgac tcgactgggg gcagatctac aaattgcagc cactttctga tgaagataag
     2641 ttgcaggcgc tacagttacg cgcgcgtttg cgtggttttg aactgccgga agatgtgggg
     2701 cgtttcttgc tgaagcggct cgacagagaa atgcgcacgc tatttatgac gttggatcag
     2761 ttggatcgtg cgtcgattac cgcgcaacgt aagctgacca ttccgtttgt gaaagaaatt
     2821 ctgaagttgt agataaggtg tttattgtcg gatgcggcgc gaacgcctta tccgaactac
     2881 gcgaagttaa tccatagtat tcgtaggccg gataagacgt ggcaagcgtc gcatccggca
     2941 atgtgcttca actagcgatg gagcacatct taacccacga tttccagtac ctgctccggt
     3001 ggacgaccaa tccgcgcttt gccattcgcg accacaatcg ggcgctccat tagcttcgga
     3061 ttatccacca ttgcctgaat cagcgcctct tcgctaagcg aactgtcagc caggttcagt
     3121 tctttataaa gatcctcttt ctggcgcatc aattcacggg cgctattcat ccccagcatt
     3181 ttcagcaaat cgcgcagcgt tgccgcatcg gcgggtgtct caaggtagag caccacttct
     3241 ggctctacgc cgttttcttt cagcaggttc agcgtttccc ggctctttga acagcgtggg
     3301 ttatggtaga tttttacctg tttggtcatt gttcttcctt taatgcgaat tacatcttgg
     3361 tataaggctt aaagcgttcc tgcagctggc gcaactggtc gatgcgcgca tcgtaacgcg
     3421 cttgttgcag gctgcctaat ttcacctgcg aactggcgct actcaacagc gaaatggcct
     3481 gatcgagtcg cccggcgagc gcataacctt ctgctcgcgc agccagctcc tgatcgcggt
     3541 tatttagcgc ggcttccgcc tgtgccagta aatcccagcc gttgctgtca tctttattat
     3601 taaaggtgta gcgattgaga atattggccg cttcttgtgg ttgaccgcct tgcagataag
     3661 cgtttgccag gttgagctgc aacaccggat tggtgcgcaa atcacgggca tttttcaggc
     3721 gattgattgc ctcattggct ttgttttgcc caagatcgat atcggtagcc agatcgagat
     3781 accatgcgtt gccaggttct gccgccagta acggttgcag cgttttacgc gcctcgtcgt
     3841 atttattggc ttccatcgcc tgtaaagcac gaccatattg cgccgctcgt tgctgacgaa
     3901 cgtttccttt cgcccattca tccagcaaat cactggtgag ctggttacgt ccggaattat
     3961 acatccctag tgtgcgcgct ttcgccagat agaaatcttc cgacgactgc accaccatcg
     4021 gtcgcatctg attagcacgg ttgcgggcat ctgccagacg actttccggc aacgggtgag
     4081 tcaataaaat ttccggcggg cgcgaggagt aacgcgcctg atcgagtaat ttttcgagga
     4141 aggttggcat cgcctgcgga tcgaatcccg agcgttgcag cacctgaata ccaatgcggt
     4201 ccgcttcctg ttcattttgc tgggtgaaac tgatcatccc ctgacgtgtt cccgccagtg
     4261 taccggtcag cgccgccatc cccgcctgcg gactggccat cgccagtaaa atagaaccta
     4321 acgcgccgac ccaggtcagc ggcgcgctgc gttgttgatc ttccatcgct cgcgccaggt
     4381 gacgttgggt gacgtgggag atttcgtgcg ccataactga agccagttga ctttcgttat
     4441 cggaataacg gaacagggca gagtgcagca ccacgttgcc gccaaagaaa gcaaaggcgt
     4501 taatttcgtc gttgttgatc agaaaaaaat gaaacggtgt cttaaccgag ttggcatgcg
     4561 aaaccagacg catccccagc gaattaatat attgcgttaa cagcgggtca ttaattaacg
     4621 gcgcgctgcc gcgtagctgg cggacataat agtcgcccat ctgcatttcc tgaccaatgg
     4681 aaagcgtgct tcctgcggag gttcccatat ccggcaaggt gtctgcgctg tcggcaaacg
     4741 ccggggctac ctgaccaatg gtcatagcag caatgagggt tgcaaccagg ttttttttca
     4801 actgcctgaa cataacctct atcctgtatt tctgaagata gtcatttgac cgatactgtt
     4861 aaatatcgtt cccaccgcgc cgcctttgag tgtaacggcg aagattagcc cactcaaatg
     4921 acttttcagt ttcctgaatc cgtcataaaa agcgaagtct tttttgtgac atttcgatac
     4981 aattgcggat cgcaatcccg ctcttgaggt tcagggaagg tttttatgct cgaaatgttg
     5041 atgcaatggt atcgccgccg ttttagcgac ccggaagcga ttgccttgct ggttatttta
     5101 gttgccggat ttggcattat ctttttcttt agtggcctgc ttgctccgtt gctggtggct
     5161 attgtgctgg cctatttgct ggaatggcca accgtgcgcc tgcaatctat tggctgctcc
     5221 cgccgctggg cgacgtcgat tgtattggtg gtttttgtcg gtatattgct actgatggcg
     5281 ttcgtggtac tgcctatcgc ctggcaacag ggcatctact taatccgcga tatgccgggg
     5341 atgctcaata agctttctga ctttgccgcc acgttgccgc gccgctatcc ggcgttaatg
     5401 gacgcgggca ttattgatgc aatggccgaa aatatgcgca gtcggatgct gaccatgggc
     5461 gattcggtgg tgaaaatttc cctcgcctcg ctggtcggtt tgctgaccat tgccgtctat
     5521 ctggtgctgg tgccattgat ggtcttcttc ctgctgaaag acaaagagca gatgctgaac
     5581 gccgttcgtc gggtgctgcc gcgcaaccgt ggactggcag gacaggtgtg gaaggagatg
     5641 aatcaacaaa tcaccaacta tatccgcggc aaagtgctgg agatgatcgt ggtggggatc
     5701 gccacctggc tggggttctt gctctttggg ctgaactatt cgctgctgct ggcggtgctg
     5761 gtcggcttct cggttcttat tccgtacatt ggcgcatttg tggtgaccat tccggtggtt
     5821 ggcgtggcgc tattccagtt tggtgcaggc acggaattct ggagctgctt cgcagtgtat
     5881 ctgattattc aggcgctgga cggtaatctg ttagtaccgg tgttgttctc cgaagcggtt
     5941 aacctgcatc cgctggtgat tattttatcg gtggtgatct tcggtggttt gtggggattc
     6001 tggggcgtat tcttcgctat tccattggcg acgctgatca aagccgtgat ccacgcctgg
     6061 cccgatgggc aaatagcgca agaataatct tcagcccgac gaactgtttc gccgggctga
     6121 ttttttaggg ttcctgacgt aaataaatag cccgataaca aatactcacc agcactgcac
     6181 cgccgataat attacccagc atcaccggga gcagattggc ggtaataaaa tggctgaccg
     6241 tcagtgccgg aaaattgtct gcgctactgt gcgccagttg ccagaagggg gttggggcaa
     6301 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaagc
     6361 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataacgaac
     6421 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
     6481 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
     6541 cccacatcgc gttttcactc atcaccagcc cggaaaaaca gattaacaaa ctgaaacagg
     6601 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
     6661 taataacgcc ccgacttttt gccattaccg tcattaccga cgaggtgaac aggctggtgc
     6721 cgcaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
     6781 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccgatcgcca
     6841 taaacacgcc agcggacatc gccattaaaa agaccgacac tttactgcgg gcaattttat
     6901 gggcggcaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
     6961 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagatcc
     7021 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccttaatcgt gtcgccgcgc
     7081 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
     7141 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
     7201 ctgaatcttc atttagcgtc ggcagtaaac ggctttgtcg gacatttaga tgtaaattca
     7261 gactgttacc acgagtcaac agtaccgccc gctcaatcac gttttccagc tcgcgcacgt
     7321 tgcccggcca atcccacgac atcaactggc gtagtgcctc ggtcgggatg gcgtcaattg
     7381 cgcgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     7441 ccggacggtc gcgcagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     7501 cgctgcgaaa ctggcgctct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     7561 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     7621 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcgagaaaca
     7681 acgtgtcgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     7741 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     7801 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     7861 cctctttgcc ggttccggtt tcaccacaaa tcaacaccgt actgtcgctc ttcgccacaa
     7921 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     7981 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     8041 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     8101 tgtgttgtag tagctggcag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     8161 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     8221 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcatctgcac
     8281 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctttcgccag
     8341 gcatacagcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctga
     8401 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     8461 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     8521 cattagtgat atccaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     8581 ccacctgttg ttcttcatca ttgagccatg attgcagaac cggggtgatt atgcgtgtca
     8641 gccagttaaa cagtgtgcaa tcctggggag taaaacgact gccgtgggtg cgaataaagc
     8701 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     8761 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     8821 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     8881 ggatgaaggc gcgtaataaa gacgccttgc cgtgtttctg tgctaaccgc tccgcgagca
     8941 ttccgtttac cgcttcaatt gttattcctt gtggcggggc aaacatcgcc tcgtctgaca
     9001 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tggccatcag
     9061 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     9121 ctgccacaca gacgtcgcgt catctgccag tggcgcgaat gcccgggtca acagtgcggt
     9181 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     9241 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc agcggaaggc
     9301 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     9361 ctgggagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     9421 gaaaacaatt tccccgcact cctcagccat gtaacccccg gagaacgcga aagagttgct
     9481 gaacgatact attgagacgc ggatcgtcag cttcgcgcaa ccaggtgtta acccgatttc
     9541 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     9601 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     9661 gccacagcgg ttgcatggtc accccggtgg gatcgcgata atccacagcg tgaatcttct
     9721 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     9781 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     9841 gatcgtggaa aatccgccgc cggcaccgca cgcgccgtag gaaacacaga ttttatgatc
     9901 gggggcagac tcatacgccc gaagtgcagg catacgcatc gcccgggtga gtgcgccagt
     9961 aaataacaaa atatcggcgt gacgcggtga tgaaacaacc ttaatgccaa aacgttctgc
    10021 gtcgaatact ggtgtaatgg cggcaaaaat ttcgatttca caggcgttgc agccaccgca
    10081 atcgacgcga taaacgtagg ctgagcgacg gatatcctgt agcaaatgcc gcttcatctt
    10141 ttgcgtttgc tcgtccagcg tgatgggctg gctgacatat tgcgtaagca ctggactcat
    10201 agctgctcct tagccaccag cggtacatct gtatcgtcgt tgagcagcgt cgcgcgttgt
    10261 ttgcactccg ggcagacgct cgcttgcgcc cataacattt cgcggttttg tggcgcattt
    10321 tgttgctgtg ccaacaattc agcagccagt gcgacggttt tttgcggggc aaacgggcgt
    10381 tcgcaacggc tgcaacgttg tagatggaac gtcgcgcggg tatagagatc ggctttattg
    10441 gtgacggtca gttcaaagtt attggtaagc tggatggctc tggtcgggca cacttcttca
    10501 caacgtccgc agtaaataca acgccccaga tagagctgcc aggtgcgcga attttgctgg
    10561 tcgtcggtct ggatagtcag cgcatttgcc ggacaagcac aggcgcaggc accgcaggca
    10621 atacattggc tgggcatcag gtccggtttc ccgcgaaagc ctgggctgac ctccagtggc
    10681 gcgaagggat atttcaccgt cgcggttccg gcgcgcataa tagttttcag taacttcagc
    10741 atcttctgtc cttacttcag cggcgaacgg ttacgatcaa tgccgtagcg ttcgatctct
    10801 ttatacggca tggtttttga ctggcgcttg cgcacatcta ccagcgtcac acggtcggta
    10861 caggagtagc agggatcaag gctaccgata atcagcggtg cgtcagaaac ggtattgccg
    10921 cgcagcatgt aacgcaacac cggccagttg gcgtaggtgg cggcacgaca acgccagcgg
    10981 aacaattttt ggttatcacc gagcatgctc cagtgcacgt cttcgccgcg tggcgcttca
    11041 acaaagccca gcgcgaatgc gtgaggttta tagctaaagc cttcggtcag cagtggggta
    11101 tccggcatgt tgtcgagggc aaattccagc attgccagcg aatcaaacgt ctctttgaca
    11161 cggaccatca tgcgggagaa gacatcgccg ccggtaaagg taaacagtgt ttttggaata
    11221 ttgccgtagt cggcgtaggg gtgatcaaag cgcaaatcac gggcaaaacc gctgccgcgg
    11281 atcagcggcc ctacagggct ataatcacgg gcgatttgtc ggtcgagaat gccaatgccc
    11341 tgagtgcgtt gttccatatt cggcgtggca agcagcatct ctaccagctc cgacacgtcg
    11401 gcgcgcatct cgcgcaccag tttcagcgtt tgcagacgtt gctctttgag aatatcgcgg
    11461 cgaacaccac caatgagatt cagaccgtag gttttacgcg acccggtcag caattccgcc
    11521 attgtcatcg acttttcccg cacgcggaaa aattgcataa agccggtatc aaatccaaca
    11581 aaatggcagg agaggccaag gttgagcaaa tgactgtgta gccgttcgac ttccagcaga
    11641 atcgagcgaa tagtatgcgc tcgttgcggc acctcaatcc ccagtgcatt ttcaaccgag
    11701 ttggtatagg ccacactgtg ggcaaaaccg caaatcccac acacgcgatc cgataagaag
    11761 gtcacttcgt tgtagcccat ccgcgtttct gccagtttct ccatgccgcg atggacataa
    11821 aacaggcggt aatcagcatc gacaatgtgc tcgccatcca caaacaagcg gaagtgaccc
    11881 ggttcatcgg aggtgatatg cagcggacct acagggatca cccgcgcatc gctgtcgccc
    11941 tcgttgataa acggatacgt ttcggtatca gtcgtcggtt cagggcgcag tcgataatcc
    12001 atcgcgtctt tgcgcagcgg atgcatatct tccggccagt catcgggcaa caccaggcga
    12061 cgctgatccg gcaggccaac cggaatcagc ccgtacatat cgcgaatttc tcgctcgccc
    12121 cagaccgcgg cagggacgcg cggtgtgact gacggaaact cccgactgtc ggcatcgacc
    12181 agtgccttca ccacgatcca gcatttttcg gccccttcca tagaaagggc ataataaacg
    12241 gcgtaatgac cgttaagtgt ccgctcgtcg ttgccaaaca ggatcggaag ccagccatca
    12301 tgttgataat aaagataatg tacaacgtca ggcagcagat tgattttcac cgtaatggtg
    12361 acctgttcag gcgtttgtcg ctcttcatcc agcaccgcgc cggggaactg cgttttcagg
    12421 gcggcgagaa tcgcttcgcc acgatttgac gatgaattaa cgttcactgt gtttctccga
    12481 aacagatgcg gtgcctgaag gcaaaaaatc atgccaggtg ctacgttgtg caggcaggtc
    12541 gtgcgtccct gagaggacta tagtggaagc gcccgccagg atcctgatga caggttgtgg
    12601 aatatgcgtt cccatcgcca gcatcatgac cagcagaatc accattggcg aggtggtcaa
    12661 ccagccgaga tcaccccggt taacggcctg cggcggtttc gccattaaca cccgcgctgc
    12721 catccgtacc aggcccgcca gcaccagcgt taacagcaat aacagcagga cgataatcag
    12781 caggtgatta cgtgccagtc cggcggtaat ggtcataaat tcgctaagaa aaatgttgaa
    12841 gggcggcatc cctgccagcg ccagcgcacc gccgccaaac agcacggcgg taaatggcat
    12901 gattttgagc atcccacaga cgacgttgag atcgcgcgtg ccgtacttga gcagtacatt
    12961 gccggaaccg cagaacagca gcgtttttgc cagactgtgg tttaagatgt gcagcagcgc
    13021 ggcaaaaatt cccagcggcc cgccaatgcc tagcgccacc gcgaccagcc ccatgttctc
    13081 cacgctggag tacgccagca gacgcttaat gtcccgctgt accagaatga aaaatgccgc
    13141 cacggcaacc gacaacatgc cgaagatgag caacaaccgg ttggggaaat cgctgccgat
    13201 ggcttggcaa ataatgatgt aatagcgaat cagcaccaac agcgcgcagt tcagcaatac
    13261 ggcggagagc agggcgctga ccggactcgg cgcttcactg tgagcatccg gcagccaggc
    13321 gtgcatggga aatagcccgg ttttggtgcc aaagccaatt agcacaaaca caaaggccaa
    13381 cagcattaat gttgggtcaa gcaaggacga ttgcttaaga acctcgctcc agaatatcgc
    13441 catttctgcc tgcggcataa cgctggcggc gttagcgtat accagcacgg taccgaacag
    13501 accaaaagcg acaccaacag tacaaataat gatgtacttc catgctgctt ccagcgatga
    13561 acgctgaccg taaatgccta ccagaaacgc cgagcttaag gtggtggctt cgatcgccgc
    13621 ccacatcaca atcaggttat tgctggtaac aaccagcagc atggtgaaca aaaacagatg
    13681 gaagaaaccg tagtaatcgc acagcgtaac gggtgaaagc tcgccgtgtg ccacttcatg
    13741 acgcatgtag ccaatcgagt aaataccggt gagaaagcca atcacaccaa gaatggcgag
    13801 gaacaaaccg cccagaccat caatatgaag ccacagtccc gcagcgaata tttctcctgc
    13861 atcagcggca gtttggacca ccctgagtgc cagaatcagc agcagtgtga tccctaagct
    13921 atgtaatacg gtcaccgtgc gagtcgcaga gagtctccgt ttccggcagg caaaacagag
    13981 cagcgaaaaa agcagcggcg tgagcaggag taaagcgaac atcacagaat aactcatctc
    14041 attatccctt cagcgcggtc aagttgttca cgtccagcgt gtcgtgagta cgccatattt
    14101 ttcttgccag taacaccatc acaatgacgg cgaagatggc gtcggtagcg atacctattt
    14161 ccaccagttc cggtgctcgc caggcaagaa gcgccagcac cagatgggag ccgttttcca
    14221 tcaggcagta accaaaaatt tgccgcagga tattgcgctg gctgacaatg cacagcaggc
    14281 caagcagaaa atgacctaac gctaccgcca gcgccggttt cagcccggta gccatcggta
    14341 gcttcacggg ctgaacgaca aatgcgcaaa gcaggacaat taacgccgcg agcagtgcca
    14401 tcattgccgg accgaataac gctttttccg ggatgttctg gggaatattt cgtgcagcgt
    14461 aagtcatgat taacggtacc agcagcactt tggtgataaa ggcgctggcg gaccagatca
    14521 gcagttgctc tgcggcgaac aggcacgaga gagtggcgaa aatagacacc agcaccagcg
    14581 actggcaggc gtaaaatccg catgacaggc gatagctttt gacgctaatc acaaacagcg
    14641 atgtcagcat catcagtccc gccagattat ttacgatcat agaaccggtc attcatcatc
    14701 tccttactgc aaccaggtcg cggcgattac gctggaaatc agcgacatca caatcagtac
    14761 aatcaacacc aggcgcattg accctggcag cggtgcggca tcggcgacgg cctcgctcgg
    14821 tttgccaggc acaacgcgac caaaccagcg aataaaccag gcgaaactgg cgaccgattc
    14881 aatcatcagc agaatcatgg cgggcagcag gatccagtac tccactgaca acgcaaaacc
    14941 ggcagcaaac agcgggaatt tactgaagaa gccgttgaac ggcggcacgc cggtaatcgc
    15001 cagcgctgcc acgcagaaac ccacgcctgg caacggcagg gtgtgcaata cgccacgcag
    15061 acgcggcaac aagcgcgtgc cgcagctgta actcagcgca cctgctacaa ggaaaaacag
    15121 gcttttagcg aacgcgtggt tgacgatgta ggcgatgctg ccctccagcg ccagccgcga
    15181 gccgaagatg gagagcgaca agccgaagaa catccagcca agttgagtga tggtcgacca
    15241 ggccagcaac cgcttcatat cctgctgtgg caaatacatc agaaagccat aaagaatggt
    15301 gaccagcgcc atgaccatgc caacgccgcc aattacatgc gggatattgc cgccgtcgat
    15361 aatggcgcgg gcaaaaatgt aaacgcccac tttcaccatc gatgcggcgt ggagataggc
    15421 gctgatcggt gtaggcgctt ccattgcgtc cggtagccac gcttgcatcg gtagctgggc
    15481 cgatttcccc cacgcggcaa acaggatgcc gccataaacc agataacgtg cgtcgccgtg
    15541 taactcgctc atcgcgctaa gcgcaaacgt tccggtctgc aaaaacagcg tggcggcggc
    15601 aagatacaac cccaacgagc cgatatgagt gataagtaac gcttttagtg ctgaacgctg
    15661 tgctttatcg ctctggtaat aactgatcaa cgcccaggag cagccgcccg taatttcaaa
    15721 aaacaacaac tgaccgagca gcgtcgagga gagtaccagt cccgccatcg cgccgataaa
    15781 caccagcaaa aatgcgtaat aacgattcgt gccgttatgc gggtgttcgc gatttttatc
    15841 cgtcagataa cccgtggagt agatcgtgac cagcaaaccg agaaacacca ccacaaacag
    15901 aatcagcgta ctcacgcgat caatgaccag gccaaacaat gccacatccc ccacgttgac
    15961 caacgtcaac gtgacggcaa ctttatcggc ctgataaaac gcggagatca gcgacaacat
    16021 gcacagcgtg gtcagcgcgg cgaacaaaac cccccattcg gcggcccgac gttgtggcga
    16081 aaacgaaacg accagtgcgc cgataaaagg cagcattaac gtcgtcagag caagattttc
    16141 catattccgt cagtgctcct tacagaccgg ttaaccagaa gacccatgca agcgcagcaa
    16201 ggctgaagcc aagccaggtc acatggtgaa tgagtaaaaa acgcccgcgt gccagcgtgt
    16261 tttctgcgat agaggccagt acaaaaatca gcaaaacctt gagcagcgta acgacaagtg
    16321 aagtcagcag gcaggcgaga gaaagttctt gcgcgcgccc aaagggcaga aacagggcca
    16381 caaacagtgc tgccatcacg acctgtttca gccccagccc ccatttcgcc agcgccagcc
    16441 cggcaccgga atattctgtc agcgggcctt cctgtacttc ctgttctgct tcagcaacat
    16501 caaagggaat ttttcccatc tcaatgaagc aggcaaaacc acaggccagt aacgccagta
    16561 cggtggttag cggcgagttc cagcccatcg ccagcgtatt gctgatcatc tcgatatgcg
    16621 tggaatccgc tatcagtgcc aataccagca gtgagagaat gagcatgggt tcgaccagaa
    16681 tgccgagcgt caactcgcga ctggcaccga ctcccgcaaa cgggcttccg gtatccagcc
    16741 cggaaagagc aaagaaaaaa cgaaacaggg caagaagata gataagggtg atcagatcgc
    16801 cgccgcccgc aaaaggggaa acggtaataa aaagtggtag ggccatcgcc agcaccagca
    16861 tgctgctgat taatacccac ggcatcaggc ggaacatcag acctgaagat ataggcgcaa
    16921 cttcctggcg tttaaacagt ttgtggatct cgcgataatc ctgccagatc cccggtccgc
    16981 ggcgggagtg catacgcgcg cgtatctgcc gggaaatacc cgtaaaaagc ggggttagca
    17041 gcagcagaat cacggcctgt gctaacgcaa aaatgaccag atatccgtcg caaagagttt
    17101 gtctcatggt gatttcctta gacggcaata gcgattagca gcacaaccag tgcggcaact
    17161 agatacaggc aatagagacg aaagtcgccg ctttgcagat gctgtatttc tttggccagc
    17221 cgttgggtgg cgctcacgat ggggcggatc acccgctcat cccagaaggg ttctgtgctc
    17281 tgagccctgg cggtgacgtg cgcaagatct ttatttagcc tcagcgtagg gtcgagctgt
    17341 tgtcgtagac gaaataacgc agagaagacc acacgcagcg gctgcatcac gccattgcct
    17401 gacggagcca tcgcgttttc ccagccgtag ccgcatgccc aggcatctcc tgtgcgacga
    17461 aacgctgcgc ggcgcgaacg acaccacagc cagtaaaggc caggcagcaa aggcatcgcc
    17521 agtagtaaca gcaacagtaa tgaaggagtg atccgtgtat gaaacgtgcc gggtacaagt
    17581 gctattccgc tggcgacagt gacgggaggg gtattggtaa acgcatgggc aatatgcatt
    17641 atcttcggtg ccagccaact ggcactaagc gcaatgaata cgcagagtgc cgcgagcagt
    17701 agcatggcga cgatcatcgt atttggcact tcctgtgcct cttcagcgtg tgtactgcgc
    17761 ggcgcaccac agaaagtaat accgtacatt ttgacgaagc acattactgc cagcccacca
    17821 gtgactgcca gcattaccat agcaatagga cccgcaagtt gtagcgctac ggcttccaca
    17881 cgacttagtg agaacagcga ctgccaggtg taccattcgc tgataaaacc attcagcgga
    17941 ggaatggctg atatcgcgag gcaaccaatc aggcatgctg cggctgtcca cggcatccgt
    18001 ttcgccagtg cccccatttt ttccatgtcg tgggtatgca aacgcgaaat aatcgcgccc
    18061 gcgccgagaa atagcagccc tttgaacacc gcatggttga gcagatgaaa cagtgcgccg
    18121 agcagtccaa ccacggtgag cagcgggtcg tgcagtgaca gaccgtccat cgccacaccg
    18181 actgccagca aaataatgcc gacgttttcg acggtactcc aggccagcag ccgtttgata
    18241 tcctgttccg ccagcgcata tagcacgccc aggagcgcgg agattgcgcc gatcgccatc
    18301 accagaatgc cccaccacag aggcaaaccc gtttgcgcca gcagatccat cgctactttc
    18361 aggatgccga aaataccgat tttgaccatt acgccagaca tcaacgccga agcgtgcgat
    18421 ggtgcggcag ggtgagcgcg cggcaaccag ctgtgcaacg gcatcatccc ggctttcgcg
    18481 ccaaaaccga aaaaggccag cagaaacacc gccgacgcca gccccggaga aagtgaaagc
    18541 gtgcgaaact ggcaaaatcg aggctgccgc tttcgcgccc catcagcaag aaggcgatca
    18601 taatcagcac cgatccggcg tgggcgataa aaaagtagag catgccagcg ttgatcgacg
    18661 ttttatcctg cctggcaatg accagaaacc aggaagacag cgacatcatt tcaaacagca
    18721 cgatgaacca aaaagcgttg tccatcacca gcagggcaac cattgatgcg atgaaaagat
    18781 tcataaagaa gcccatcgcc gccgcgcctt tgccctcgta ttcgcgcata taagtcaatg
    18841 aatagagcga acaaaccacc accagcaagg agataaccag caccataaat gccgagaggc
    18901 tatccattcg tagggttaaa tcggcaaaat caaaaggcgt ggcaaaacgg gcgactaaag
    18961 ttacgcctgc atgtaattgc gtaattccgc tgatgatgcc aatcacgccg cccaccagcg
    19021 atgtgatgcc ggaaagctta atagccagtc tgtccagacc gagtaaaaac agcgaagcca
    19081 gactggcaaa cagatagagg atcagcgacc aggttaataa ttgcagggca tccattagcg
    19141 ttgctcctga gtgagggaaa ggggatcctc cccgccattg gcaaaccagc ttgcggcaag
    19201 gcgctgtttt tctttcatct ggcgttgcag gctatcgtcg gtgatcagcc gtaacgcctg
    19261 attcgggcaa gcgcgaacgc aggccggacc ttctggcaag aaatcacaca ggtcgcattt
    19321 cactgcgacg gtctggacac ccggttccca gcgcagcaaa gcatgttggg ttggcgcgtt
    19381 ttcttcgccg tctttgagtg agccctcagc ccgaaaaaca tattgcgcag gggcattcac
    19441 cggacggctt cctgaagcgc tgattgcgcc aaatgggcag accacggcgc aaagcttgca
    19501 gccaatacaa aggctttcgt tgagttgaat cgcatcgtcc ctctgggaga tggcattgac
    19561 cgggcagacc tgcaggcaag gggcttcctc acagtgatga cacacgacag gggcagtgag
    19621 tgttgacgtc ttcgccaggg ccaggcgcgg gtgttgctgt aaaccttgcg ttttatgcac
    19681 gtccgaacag gcagcgagac aggtattaca tcctgtacac cacagtggtt cggccaccac
    19741 aaagcggttc atgtcggctc cttgaaaatg atatgaaaca ttgcatttca tattgcataa
    19801 aggatgccag tcttattaat ttaatatatt caataagtta tgaattaact ggattctgaa
    19861 cggtacaaaa atgatggtca ttaacgttca gcaaaaaatg gattcgacac ccgtgtcgca
    19921 ggggaaagcg agaatgtgat cttgatcacg agaagtgagg tgtgctgacc gcaagcgcag
    19981 ccagcacgga atggagcaaa gtaatcaggc gtgttctttc agccagttca gcacaacgtc
    20041 gtggtgattg ctggttttaa agtcgtcaaa gacatgttcg attttgccat cagcgtcaat
    20101 caggaagctg atgcgatgaa taccatcgta ggttttgccc atgaaggact tttcacccca
    20161 tacgccgaat tgttcgcaca cctggtggtc ctcatcagac aggagcgtaa agttaagcag
    20221 ctctttttcc gcaaaacggg agagtttttc gggtttatcg gtgctgatac ccagcacatc
    20281 aacgcccgct tttttcaact catccatgtt atcgcgtaag ccgcaggcct gtacggtaca
    20341 gccgggggtc atggctttcg ggtagaaata aaccagaaca cgctgtccct ggaagtcggt
    20401 caaattaact tgttctccgt cttgatccgg caagctaaat ttcggtgcga tatcaccggc
    20461 tttcagtgga ttcattactt aactccatcc tgttcatcat gttgggaata attgacgacg
    20521 ttaatactgc cttgtgcatt gagttctgta catagggctt tgaacgcttg ctcaatattt
    20581 gctgcgtccg cagatgcggg gctgtgggcg gttatctgaa tatgcaactg cgcagcccgt
    20641 tcattttcag caggttgcgt gcgcgacacc agctccgcaa tgttcatatg atgcgcgtcg
    20701 aaaagtgctg tgaagcgttc aattaaatgc ggggagtctg ccacatcgac ctgaacccag
    20761 acagatgctg gcattggcgg acgcggacgc gccgtcgtgc gcttcatcac gattaaaaga
    20821 tccagttcgg cacctttcaa cggtaacgtt gattcaatca gagtgatggc attccatgaa
    20881 ccggaaagca gcatgataaa cgtgaactct tctcccagca tcgccaggcg actgtcttca
    20941 atattacagc cgcaactact gacatgacgg gtgatggtgt tcacaattcc agggcgatcg
    21001 gcacccaacg cagtgatcac cagataatgt tgcgatgaca gtgtcaaact ggttattcct
    21061 ttaaggggtg agttgttctt aaggaaagca taaaaaaaac atgcatacaa caatcagaac
    21121 ggttctgtct gcttgctttt aatgccatac caaacgtacc attgagacac ttgtttgcac
    21181 agaggatggc ccatgttcac gggaagtatt gtcgcgattg ttactccgat ggatgaaaaa
    21241 ggtaatgtct gtcgggctag cttgaaaaaa ctgattgatt atcatgtcgc cagcggtact
    21301 tcggcgatcg tttctgttgg caccactggc gagtccgcta ccttaaatca tgacgaacat
    21361 gctgatgtgg tgatgatgac gctggagctg gctgatgggc gcattccggt aattgccggg
    21421 accggcgcta acgctactgc ggaagccatt agcctgacgc agcgcttcaa tgacagtggt
    21481 atcgtcggct gcctgacggt aaccccttac tacaatcgtc cgtcgcaaga aggtttgtat
    21541 cagcatttca aagccatcgc tgagcatact gacctgccgc aaattctgta taatgtgccg
    21601 tcccgtactg gctgcgatct gctcccggaa acggtgggcc gtctggcgaa agtaaaaaat
    21661 attatcggaa tcaaagaggc aacagggaac ttaacgcgtg taaaccagat caaagagctg
    21721 gtttcagatg attttgttct gctgagcggc gatgatgcga gcgcgctgga cttcatgcaa
    21781 ttgggcggtc atggggttat ttccgttacg gctaacgtcg cagcgcgtga tatggcccag
    21841 atgtgcaaac tggcagcaga agggcatttt gccgaggcac gcgttattaa tcagcgtctg
    21901 atgccattac acaacaaact atttgtcgaa cccaatccaa tcccggtgaa atgggcatgt
    21961 aaggaactgg gtcttgtggc gaccgatacg ctgcgcctgc caatgacacc aatcaccgac
    22021 agtggtcgtg agacggtcag agcggcgctt aagcatgccg gtttgctgta aagtttaggg
    22081 agatttgatg gcttactctg ttcaaaagtc gcgcctggca aaggttgcgg gtgtttcgct
    22141 tgttttatta ctcgctgcct gtagttctga ctcacgctat aagcgtcagg tcagtggtga
    22201 tgaagcctac ctggaagcgg caccgcttgc ggagcttcat gccccggctg gaatgatttt
    22261 gccggtgacc tccggtgatt atgcaatccc ggtgaccaac ggtagtggtg ctgtcggtaa
    22321 ggcgctggac attcgtccac cagcccagcc gttggcactg gtttctggcg cgcgtaccca
    22381 gttcacgggc gataccgctt cattgctggt ggaaaatggt cgtggcaata ctctgtggcc
    22441 gcaggtggtt agcgtgctgc aggcgaaaaa ctacaccatc acccaacgtg atgatgctgg
    22501 tcagacactg accaccgatt gggtacaatg gaaccgtctg gacgaagacg agcagtatcg
    22561 tggtcgttat caaatctctg ttaagccgca gggttatcag caggcggtta cggttaaact
    22621 gctgaacctg gaacaggcgg gcaaaccggt tgcagacgcg gcttccatgc agcgttacag
    22681 cacggagatg atgaacgtta tttccgccgg tctggataaa tctgccactg acgccgcgaa
    22741 cgctgcgcaa aatcgtgcct ccaccactat ggacgtacaa agtgcagctg atgacaccgg
    22801 tttaccaatg ctggtcgtac gcgggccgtt caatgtggtt tggcaacgtc tgccagcggc
    22861 gctggaaaaa gtgggcatga aagtgaccga cagcacccgt tcgcagggca acatggccgt
    22921 aacttataag ccgctgtctg acagcgactg gcaggaactg ggcgcgagcg atccaggcct
    22981 ggcatccggt gactataaac tgcaggttgg cgatttagat aaccgcagca gcctacagtt
    23041 catcgatccg aaaggtcata ctctgactca gagtcagaac gacgcgctgg tatctgtctt
    23101 ccaggctgcg tttagcaagt aaaaatacag ggctggaatc atccggccct tttttctgat
    23161 atgatacgca aacgtgtgcg taggcaggaa aacgcgattt tagcggtaat tcgcacgaaa
    23221 tttgtttgtc ggacgtagtt cgaataaggc gttcacaccg catccgacaa aacatccggc
    23281 acaccagaca gcaaaagatt ttaaaacgtt aattcacacc caggagtgat aaagatgcaa
    23341 aagcaagctg agttgtatcg tggtaaagcg aaaaccgtat acagcacgga aaacccggac
    23401 ctgttggtgc tcgaattccg caatgatacg tcagcagggg atggcgcgcg cattgagcag
    23461 tttgatcgca aaggtatggt gaacaacaag ttcaactact tcatcatgag caaactggct
    23521 gaagcgggta tcccgactca aatggagcgt ctgctctccg ataccgaatg tctggtgaaa
    23581 aagctggata tggtgccggt tgagtgtgtc gtgcgtaacc gtgctgcggg ctctctggtg
    23641 aaacgtcttg gaatcgaaga aggtattgag ctgaacccgc cgctgttcga tctgtttctg
    23701 aaaaacgacg ccatgcacga tccgatggtc aacgaatctt actgcgaaac ctttggctgg
    23761 gtaagcaaag agaacctggc gcgtatgaaa gagctgacct acaaagcgaa cgacgtgctg
    23821 aaaaaactgt tcgatgatgc tggtctgatt ctggtcgact tcaagctgga atttggtctg
    23881 tacaaaggcg aagtggtact gggtgatgag ttctccccgg acggtagccg cctgtgggac
    23941 aaagaaacgc tggagaaaat ggacaaagac cgtttccgcc agagcctcgg tggcctgatc
    24001 gaggcctatg aagccgtcgc ccgccgcctg ggtgtacagc tggactgatt tttctgttca
    24061 tcatcttgcc gtgctgctgg cacggcaaga caaccattct cgtaagatgt gcattgaacg
    24121 ctcattcctc cgccatttca tcccgctttc ctgtggtaat cttcctctga accacctacg
    24181 atcatttcta taatgaatat attgttgagg tatctatgcg ttggcaaggg cgacgtgaaa
    24241 gtgacaatgt tgaagacagg cgcaacagct ctggcggtcc gtcaatgggc ggtcccggtt
    24301 tccgtctgcc gagcggtaaa ggcgggctga ttttactgat agtcgtgctg gttgcaggct
    24361 actatggcgt tgatttaacc gggttgatga ccgggcagcc ggtttctcaa caacaatcaa
    24421 cgcggtcaat tagccctaat gaagacgaag ccgcaaaatt cacctcggtg attctggcaa
    24481 ccacggaaga cacctgggga caacagttcg aaaagatggg taagacctat cagcaaccga
    24541 agctggtcat gtaccgtgga atgacgcgca ccggctgcgg ggcgggccag tccattatgg
    24601 ggccgttcta ttgcccggcg gatggcacag tttatatcga tctctccttc tatgatgaca
    24661 tgaaagacaa acttggcgcg gatggcgatt ttgcccaggg gtacgttatc gcccatgaag
    24721 tcggtcatca tgtgcagaaa ctgttaggca tcgagccgaa agttcgtcaa ctgcaacaaa
    24781 acgcgacgca ggcggaagtg aaccgcttat ctgtgcgtat ggaactccag gccgactgtt
    24841 ttgccggtgt ctgggggcat agtatgcagc agcaaggcgt tctggaaacc ggcgatctgg
    24901 aagaggcgct gaatgcggcg caggccatcg gcgatgaccg tttacaacag caaagtcagg
    24961 ggcgagtagt accagacagt ttcacacatg gcacttctca gcaacgctac agctggttta
    25021 aacgtggttt cgacagcggc gatccggcac aatgtaatac ttttggtaaa agcatttaat
    25081 ttccggggca gggatggctg aactgactgc gctttacaca ttaacagcgc aaatgaaacg
    25141 tgaagggatc cgccgcttgc tggtgttgag cggggaagag ggttggtgtt ttgatcatgt
    25201 tcttaagttg cgtgatgcct tacctggcga ctggctgtgg gtttcgccgc agccagatgc
    25261 tgaaaaccac tgttcaccct cggcgctaca aactttactt gggcgcgagt tccggcatgc
    25321 ggtattcgac gcccgccacg gctttgatgc cgctgccttt gcagcactta gcggaacgtt
    25381 gaaagcggga agctggctgg ttttgttact ccctgtatgg gaagagtggg aaaaccaacc
    25441 tgatgccgac tcgctgcgct ggagtgattg ccctgaccct attgcgacgc cgcattttgt
    25501 ccagcatctc aaacgcgtac ttacggcgga taacgacgct atcctctggc ggcaaaacca
    25561 gccgttctcg ttggcgcatt ttactccccg tactgactgg caccccgcga ccggcgcacc
    25621 acaacaagaa caacagcaac tcttacagca gctactgacc atgccgccgg gcgtggcagc
    25681 ggtaacggct gcgcgtgggc gcggtaagtc ggcgctggca gggcaactca tttctcgtat
    25741 tgcgggcagt gcgattgtca ccgcgcccgc aaaagcggca acggatgtac tggcacaatt
    25801 tgcgggcgag aagtttcgct ttattgcgcc ggatgccttg ttagccagcg atgagcaagc
    25861 cgactggctg gtggtcgacg aagccgcagc catacctgcg ccgttgttgc atcaactggt
    25921 atcgcgtttt cctcgaacgt tgttaaccac tacggtgcag ggctacgaag gcaccggacg
    25981 tggttttttg ctgaaatttt gcgctcgctt tccgcattta caccgttttg aactgcaaca
    26041 gccgatccgc tgggcacagg gatgcccgct ggaaaaaatg gttagtgagg cactggtttt
    26101 tgacgatgaa aacttcaccc atacaccaca aggcaatatc gtcatttccg catttgaaca
    26161 aacgttatgg cgaagcgagc cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc
    26221 gcactaccgg acttcgccgc tggatttacg ccggatgatg gatgcaccag ggcaatattt
    26281 tttacaggcg gctggcgaaa acgagattgc tggagcgctg tggctggtgg atgagggggg
    26341 attatctcaa caactcagtc aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct
    26401 ggtggcccag tcgctggcgg cgcacggtag caatccactg gcagcgacat tgcgtggacg
    26461 gcgggtcagc cggatagcag ttcatccggc gcgtcagcgg gaaggcacag ggcagcaact
    26521 tattgctggt gctttgcaat atacgcgtga cctcgactat ctttcggtga gttttggtta
    26581 caccggggag ttatggcgtt tctggcaccg ctgcggtttt gtgctggtgc ggatgggtaa
    26641 tcatcgtgaa gccagcagcg gttgctatac ggcgatggcg ctgttaccga tgagtaatgc
    26701 gggtaaacaa ctggctgaac gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc
    26761 aaagtggaat ggcgaaacgc ttcctgttga tccactaaac gatgccgtcc tttctgacga
    26821 cgactggctt gaactggccg gttttgcttt cgctcatcgt ccgctattaa catcgttagg
    26881 ttgcttattg cgtctgctac aaaccagtga actggcatta ccggcgctgc gtgggcgttt
    26941 acagaaaaac gtcagcgacg cgcagttatg taccacactt aaactttcag gccgcaagat
    27001 gttactggtc cgtcagcggg aagagaccgc gcaggcgctg ttcgcactta atgatgttcg
    27061 cactgagcgt ctgcgcgatc gcataacgca atggcaattt tttcactgac tccttcaatt
    27121 ttctcccatg gtcattgtga gtcagcggcg taaaattaat gacatcagtt aaggagttcg
    27181 ccatgaaaca tgaccatttt gttgttcaaa gcccggataa acctgcgcaa cagttgttac
    27241 tgcttttcca cggtgtcggg gataacccgg tggcaatggg ggaaataggt agctggtttg
    27301 caccgctgtt tcctgatgcg ctggtggtga gtgtcggtgg cgcggagccg agcggtaatc
    27361 cggcggggcg tcagtggttt tcggtgcagg gtattacgga agataatcgc caggcgcgtg
    27421 tggatgccat catgccgacg tttattgaga cggtacgcta ctggcagaaa cagagcggag
    27481 taggggctaa tgccacggcg ctaattggtt tctcacaagg cgcgattatg gcgctggaga
    27541 gcattaaagc cgaaccgggc cttgcttcac gcgtcattgc ttttaacgga cggtatgcga
    27601 gcctgccgga gactgcgtct acagccacga caattcacct gattcatggc ggtgaagatc
    27661 cggttatcga tctggcgcat gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg
    27721 acgtgacgct ggatatcgtg gaggatttag gccatgcgat cgacaatcgc agtatgcaat
    27781 ttgccctgga tcacttgcgt tataccattc cgaaacatta tttcgacgaa gcgctaagtg
    27841 gcggtaagcc tggcgatgat gatgtgattg aaatgatgta aaagacagat tacctggcct
    27901 gataagacgc gtgagcgtcg catcaggcat cgttcaccat tgccggatgc ggcgttaacg
    27961 ccttatccgg cctacggttg agcactcagc ttacttcttc ggttgatcct ttttcggcca
    28021 gtcgtcgtca tcgtcccact tatcgttgaa atcacgatgc gggggaagct ccggcttgtt
    28081 ggcgagaaat ttcttatggt cgacgcgctt gagatcttta atcacgttca gcaggacgcc
    28141 taccaaaaag acaatcacca gaatccacca atatttagcc agccagtcca tgcttatttc
    28201 ctcttgcaga accactcatc aggcgacgag ctgttccatg atacgttgat acatacgggc
    28261 aagtagctgc aggtcggcag cgttcacaca ttcattaatt ttatgaatag tggcattgac
    28321 cggcccgagt tccaccacct gcgcccccat gcgagcaata aagcgcccgt cggacgttcc
    28381 gcctgtggtc agtagctgcg gtttaatttc attatagtgc tcaaccgcgt taacgaccgc
    28441 atccaccagt ttaccgcgcg cggtcaaaaa tggctgcccg gaaagccacc agtccaccgt
    28501 ataacgcagt tgatgttttt caagcagggc aagcacctgt gctttgatca tctcatcagt
    28561 cagttcggtg ctgaagcgga agttaaactg cacaaacagt tcacccggaa taacgttgtt
    28621 actgcccgtt cccgcctgaa tattggcaat ctgcatactg gtcgccggga agaattcatt
    28681 gccctgatcc cactcaatag ccactaattc attaaggaaa ggtgctgcgc gatgtaccgg
    28741 attgtcagcc agatgcgggt aggcaacatg cccctgaacg ccatgaatgg tgaggttgca
    28801 ggttaacgat ccgcgacgac catttttcac cacatcacct accacttcga tactcgacgg
    28861 ttcgccgacc aggcagtaat cgagacgctc attacgtgcc attaacgctt cgacgacttt
    28921 taccgtaccg ttgtgtgcac tggcttcttc atcagaggtg atcagaaatg ccagtcgccc
    28981 cgtatggttg ggatgttgtg cgacaaagcg ttctgccgct accaccatcg ccgccagcga
    29041 gcctttcata tctgccgcac cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa
    29101 cggcggattg atccaacgat cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa
    29161 ggctaacgtt tcaccttgcc cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat
    29221 gcgttcaacg gtaaaaccga tcgcctgcaa acgttcaatc aacaaagcct ggcatcctgc
    29281 gtcatcagga ctcagggaag ggcggcgaat aagctgttgt gtcagctcaa taaccgggca
    29341 cgacatagac tacacctcat ggaaaaattg ctgataactg gaatcactga aacccagcag
    29401 cataggctta ccaggcgcgc agagcaatgg acgtttgata attgcaggca tttcagtcat
    29461 taatgccgcc gcagaggccg catcggtgat tttattgcgg gtggtttcgt ccagtttacg
    29521 ccaggttgta ccacgggtgt tcagtaacgc ttcccagcct aattcgttga taaaaccgtt
    29581 caataattcg ctgtccagcc catcgacgcg gtaatcatga aaacgatagt cgatgttatt
    29641 ggcttccagc caacggcgag cctttttaat ggtgtcacaa tttttgatgc cgtaaagtgt
    29701 aaccataatg aatccttttt cgatctatga gaataaatac tcatttaagg gtgaaaacat
    29761 tttgcctgca aatgattatc aggaaatata acattatcga agtattcaaa ttaacaacta
    29821 ttaaataata ttaaacattt ttaacgtaaa cattaagaat attcatcttg ttatctgtct
    29881 gtattttaac cagaattaaa tcagtattag atgccgtatg aatatctcga aatgttgctg
    29941 tgtatcacat acaaattatc accgcaggac catagtattt acataaggtt tgtcccccgg
    30001 aggtagtcat cgcagcaatc gagtaaatta gcttcaccac ggtatattgt ttccgtagaa
    30061 tacccataat tataagagag gttgttatga ttgaacgtga actggggaac tggaaagact
    30121 ttatcgaagt tatgcttcgt aagtaattcc tgagacaaag tacaaacagc aagaacccgc
    30181 tgagcaggtt cttaatcgga aagttgaaaa aggcgacaca ttagcatgtc gcctttttta
    30241 ttgcttattc cgggcgcggc ttcagcggga agcggcggcg caccagtaca aagaacagcg
    30301 gcacgaagta aatagccaga atagtggccg aaatcatccc gcccattacg ccagtaccca
    30361 ccgcatgctg accaccggaa ccggcacccg tgctggttgc cattggcaat acgccgaaga
    30421 taaatgccag cgaggttatc agaatcgggc gtaaacgctg acggcaggcg tggagcgtcg
    30481 cttcaaacag gtcgtggcct ttttggttca tctcgttagc aaactcgacg atcaggatgg
    30541 cgtttttcgc cgataaacca atgaccgtta acaggcccac ctggaagtaa acgtcgtttt
    30601 ccagcccgcg catccaggtt gccagcagcg cgccgataac ccccagcggc acgaccagca
    30661 ttaccgagaa cggcaccgac cagctttcat acaacgccgc cagacacagg aataccacca
    30721 gcaaggaaat ggcgtacagc gccggagcct gcgcgccgga aagtcgctcc tgatacgaca
    30781 tcgccgtcca ctccagacca aagccgtttg gcaactgctt cactaacgat tccataatat
    30841 ccatcgccgt accggtactg acccccggtg cggcttcccc aacaatctca accgcagaat
    30901 aaccgttata gcgttccaga cgcggcgagc ctgtttccca gcgtgaggtc gcgaaagcag
    30961 agaagggcac catgccgcca tctttatttc ggacatacca gagattgatg tcatctggca
    31021 gcatgcgata cggcgcagct gcctgcacat agactttctt cacgcgaccg cgatccataa
    31081 agtcattcac atagctcgaa ccccaggcgg tttgcagtgt gtcgttaata tcgtcgatag
    31141 caacgcccag cgcctgagct ttacgctggt cgatatcaat ctgcaactgc ggactgtcgt
    31201 cgaggccgtt atggcgcaca cggggtagct ccgggttttc cgccgccagc gccagcaact
    31261 gattacgtgc tgccatcagc gcatcgtgac ccgctccagc gtggtcctgc aactccatat
    31321 caaaacctgc agaactacca agaccgctaa ttgctggcgg gctgctggcg ataacgcgag
    31381 cttctttaat ttggttaaac gccttcgttg cacgctcgat aatggcaaac gaggtgccgg
    31441 tcttactgtc gcgttcgctc cagtctttca ggcggataaa cattcgcgcc acgttttgcc
    31501 cgttaccccc agggccagaa ccaacggtgg caaacaccga catgatgttg tctttttcat
    31561 gggtgaagta atatttctcg atttgctcaa cgactttcag ggtctgttgt tgggttgaac
    31621 cgctgggcaa ttgtaccgag gtagtaaaca tgccacggtc ttccagcggt aagaacgacg
    31681 tcggcaaacg caggaacagg aacaccatgc cgccaagcag caggacataa atcacaatcc
    31741 agcgcaggct acggtggaga attttcgcca cccctttttc gtagcgttcg gcgttgcggt
    31801 taaacatctg gttaaaccag gcaaaaaagc ctttttgccc gtgatgctca ccttttttca
    31861 gtggcttaag tagtgtggca cacagagccg gagtgaggat catcgctacc agtactgaca
    31921 gcaccatcgc cgcaacaatg gtaatagaga actggcgata gatggcaccg gtggtgccgc
    31981 cgaagaaggc cattggtaca aataccgccg aaagaaccat cgcaatcccg accagtgccc
    32041 cctggatctg ccccatcgat ttacgtgtgg cttcgcgagg agtgagtcct tcctcactca
    32101 taatacgttc gacgttttcc accaccacga tggcgtcatc caccagcaga ccgatcgcca
    32161 gcaccatcgc gaacatggtt aaggtgttga cgctgtaacc gaaggcgtaa agtacggaga
    32221 aggttcccat caacaccacc ggcacggcga tagtgggtat cagcgtggcg cggaagtttt
    32281 gcaggaacag atacataacg aggaaaacca gagcgatagc ttccagcagc gttttcacca
    32341 cgtcttcaat ggaggcttta acaaacgagg tggtttcata cgccaccttg tattccagtc
    32401 cgtgcgggaa gtactgcgcc agctcgtcaa gacgattgag caccagctcc gctgtcgcca
    32461 tttcgtttgc gccggaggcc agttttaccc ccagcccgga ggctggctta ccattgaagc
    32521 ggctaagata atcgtatttc tccgccccca tttcgacggt ggcgacatcg cccagcctta
    32581 cctctgagcc gtcttgattc accctcaagg tgatatcgcg gaactgttct ggtgtttgca
    32641 gcagtgactg ggcgttaatg gtggcgttga gcgcctgctt atcgacggaa ggtgtaccac
    32701 caagttgccc aaccgcaatc tgcgcgttct gtgactcaat ggcatcagtg acatctttag
    32761 ccgtcatctg gaaactgttg agtttcgccg ggtccagcca gatacgcatg gaatattgcg
    32821 aaccataggc atcgatattc ccgacgccat tcacgcggct taacgggtcc tgaatattac
    32881 tggcaacata atcggcaata tcctgtttat ccatcgaacc atcggtagag acgaaagcaa
    32941 tggtcagaat attggtatcg ccggttttac gcaccgtcac gccctgattt tgcaccgcct
    33001 gcggtaactt tcgcatggct gattgcagct ggttttgtac ttgctgcacg gcttcgtccg
    33061 gatcggtgcc tgctttaaaa cttaaagtga cagatgcctg accggtgcca ctgctctgag
    33121 atgacatata catgagatta tcgaggccgg tcatattttg ctcgataacc tgggtcacgg
    33181 tgttttccag cgtctgggcc gatgcgccgg gatagttagc ggtcactcgc acattcggtg
    33241 gcgcgagatc ggggtattgt tcaacgggca atgagaaaat cgccagggta cctgtcagac
    33301 acaacaggat tgccagcacc caggcaaaaa tggggcgatc aataaagaaa ttcgccatta
    33361 aaagaggacc tcgtgtttct gcgtatcgtt gtaggcttga cttgcttcac tgtagcggca
    33421 aggtacgagc caatcgtgga gaaaaaaagg agttaatgta aattatcatg cccttttata
    33481 ctgaatctta agtgcaagta ttctttggtc agtaggaggc attacatcag ctgactttcc
    33541 tcaccctccg cggagcgaaa gctaatactc actaacgtgc cgccgccgga aggttgtgaa
    33601 aaagtcagcg tcccacccag ccgttccgcg cgttcgcgca tgatattcag accataatga
    33661 ccttcgggct ctttcggttc accgatgcca atcccgttat cacggatata aaccgtgtga
    33721 ttgccgtccg gcgcggtgac gcaactgacg gcgatttcac tggcgttggc gtgcttcatt
    33781 gcattcagca ccgcttcgcg aataatttgc aacaaatgca cctgcatttg cgcatccagc
    33841 gccagggttg gcagacggca gtcgagggtc agtttggcgc tggtttgatt ttgtaacgta
    33901 tccagcattt ccctcaacgc ggaggggaga tccgcctgct gcagcgtcag gcggaaagta
    33961 gtcaacagct cgcgtaactg ccgataagca tcattcaacg cctgggaaaa atcggccatg
    34021 atactttgtg cggtggcgtt atcttccggt atcgaacgct tcagtaacgt caactggata
    34081 cgtaagtaag aaagtacctg agccagcgag tcgtgcaatt cgcgggcgat ggttgcacgt
    34141 tcttccatca acaataactg ctgaaaatgc ttctgcgcct gattaaagta caaaccgcgt
    34201 cccagcatcg acgaaacgct gttaagcagc ggttctgatg atgaaacgtg actattttgc
    34261 cagtgcagtt cgccgtaaac cgtctcttgc attgtcaccg gtaaaatctg catcggcaat
    34321 tccgggtttg gttgcccttc gctaatccgc cagttttcac cgacatttaa ctccagatat
    34381 tcagccgctt cattgtcgcg aacaatctgc aaaatatggc ggaaacaatg cacatcaatc
    34441 tggctggtgt tcagcgcctg ggaacattga tacaacacct ccagacgacg cttggcctcg
    34501 tggagatcgc gggtcttttc ttctactgac gcctccagcg aaaggtacaa tttatgcagc
    34561 tcgctcgaca tctggttaaa ggtttttgca agcagaccaa gctcattcgg cagactggta
    34621 tccagcggcg gcgagtcgaa ctgcccgtgt tcaatacgtt gactggcggt aaccagctga
    34681 ttcagcgggg caaccacctg atggcgtata cggcgcaaag taaaaaagac cagcgtgaaa
    34741 ataccgatac cgccagccag ggaaatcgcc accaccagca gcattttgcg ttcagcgtag
    34801 tgctgtaaag ccagcacgaa caggtctatc tgattaacat aattattaat attggcctga
    34861 taccacggca aatcgccctt gctgagccga ttattcatct ccagccagtt ggcattcaga
    34921 tgcgcatagc gagtttttac tgcttctggc acataccaga cgttgagatt ggttaatacc
    34981 ggtgaatgca gtgcctgctg aaacaactgg cgatgtgcat tgagttgtgg actgccactt
    35041 tgcaagtcgt agcccaggcg gtaactctgc atacgtagcg atccggcaat attgatagcc
    35101 tcagcgtcgc gcaaactgct cgccagagtt agcagagcga tacccgtgga aagaatcgac
    35161 agcagcacaa tgtaaaaaaa ggcccgggcc agactggccg agacgggtcg tttaacaatc
    35221 acacgcgtct tctccacaaa aaatcattaa tgttccagtt agctgaaaca caatttagtc
    35281 agtgatggga ttatttgatc tgccacagtt tatagcgaaa tagttactta ttctgggcac
    35341 atgaacattg ccgattcccc tgtaagtcgg gtaataacaa cactcatata aagaataagg
    35401 tttttacaac caaaaaagaa ggtcgttatg aatcgtttta ttatggccaa cagtcagcaa
    35461 tgtctgggtt gtcatgcttg tgaaatcgcc tgtgtcatgg ctcacaatga tgagcaacat
    35521 gtcctgagcc aacaccattt tcatccccga attacggtta tcaaacatca acagcaacgt
    35581 agtgcagtga cctgtcacca ttgtgaagat gcgccctgcg cccgtagctg ccctaatggc
    35641 gcaatcagcc acgttgatga cagcattcag gtcaatcagc aaaagtgtat tggctgtaaa
    35701 tcctgcgtgg tggcctgtcc ttttggtacg atgcaaatcg tcctgacacc cgtcgcggca
    35761 ggaaaagtaa aagccacggc gcataaatgc gacctttgtg cggggcgcga aaacggtcct
    35821 gcctgtgttg agaattgccc ggcggacgcg ctgcaactgg tcactgacgt cgcactctcc
    35881 ggcatggcga aatcccgccg cttgcgcacc gcgcgtcagg aacatcaacc gtggcatgcc
    35941 agtaccgcgg cgcaagaaat accggtaatg agtaaagtcg aacaaatgca ggcaacgccc
    36001 gcgcgtggcg agccggataa actggcgatt gaagcgcgca aaaccggttt tgatgaaatt
    36061 tatctgccat ttcgcgccga ccaggcacaa cgggaagcct cgcgctgcct taagtgcggc
    36121 gagcacagcg tttgtgaatg gacctgcccg ctgcataacc atataccgca gtggattgaa
    36181 ctggtgaaag ccggaaacat cgacgccgcc gtcgagcttt ctcaccagac caacaccctg
    36241 ccggaaatta ccggacgcgt ttgtccgcaa gaccgtttgt gtgaaggtgc ctgtactatt
    36301 cgcgatgagc acggcgcggt aactatcggc aacattgaac gctacatttc agatcaggcg
    36361 ttggcgaaag gttggcgtcc tgacttaagc catgtcacca aagtggacaa gcgggtggcg
    36421 attatcggtg caggtccggc agggctggcc tgtgcggatg ttctgacccg caatggcgtg
    36481 ggggtgacgg tgtacgatcg ccatccagaa atcggtggct tgctcacttt cggcattcct
    36541 tctttcaaac tggataaatc cctgctggca cgccgtcggg aaatcttcag cgcgatgggg
    36601 attcacttcg aactcaattg tgaagtgggt aaagatgtct ctttggattc gcttttggaa
    36661 caatacgacg cggtcttcgt tggcgtaggc acttaccgtt ccatgaaagc gggtttaccc
    36721 aatgaagatg cgccgggcgt ttatgacgcg ctgccgttcc tcattgccaa cactaaacag
    36781 gtgatggggc tcgaagagct accggaagag ccgtttatca ataccgccgg acttaacgtc
    36841 gtggtactgg gcggcggcga caccgcgatg gactgtgtgc gtaccgcact gcgccacggc
    36901 gcgagtaacg tcacctgcgc ttatcgtcgt gatgaagcta acatgccagg ctcgaagaaa
    36961 gaagtgaaga acgcccgcga agagggggcc aacttcgaat ttaacgtcca gccggtggcg
    37021 cttgagctga atgaacaagg tcacgtctgc gggattcgtt tcctgcgcac gcgtcttggc
    37081 gagccggatg cccaggggcg tcggcgtcca gtgccggtgg aaggcagtga atttgtcatg
    37141 ccagccgacg cggtgattat ggcgtttggc ttcaatccgc acgggatgcc gtggctggag
    37201 tcgcacggtg taacggtaga caaatggggc cgcatcatcg cggatgtgga aagccagtac
    37261 cgttaccaga ccaccaatcc gaaaatcttc gctggtggtg acgccgtgcg tggtgcggat
    37321 ctggtggtta ccgcaatggc agaaggacgt catgcggcac aggggattat tgactggctg
    37381 ggggtaaaat cagtcaaatc tcactgatag cctgcgcaga caaacccgac ttcacagcgt
    37441 aagataattg ttcatttcgc gctgtggagt cggtatgacg caacaaatca ccctcattaa
    37501 agacaaaatt ctctccgata actatttcac cctgcacaac attacttacg atctcacgcg
    37561 caaagatggc gaagttatcc gccataaacg tgaagtttac gatcgcggta atggtgcgac
    37621 gatcctcctg tacaacgcga agaaaaagac cgtggttctg attcgtcagt tccgtgtcgc
    37681 tacctgggtt aatggcaatg aaagcgggca gctgattgaa acctgcgccg ggctgctgga
    37741 taacgacgaa ccggaagtgt gcattcgcaa agaagcgatt gaagagacgg gctatgaagt
    37801 gggcgaagtg cgcaaattat ttgaactgta tatgtcgcca ggcggtgtga ctgagctaat
    37861 ccactttttt atcgccgaat acagtgacaa tcagcgcgct aacgccggtg gcggtgtcga
    37921 agatgaagat attgaagtgc tcgagctgcc gttcagccag gcgctggaga tgatcaaaac
    37981 cggcgagata cgtgacggta agacggtgtt attgcttaac tatttgcaaa cgtcacattt
    38041 aatggactga aaaataacaa taatatttcg ttgtttatta ttggctcaat ccgacaaatc
    38101 cgattgagcc gcgctacctg cgcaacgaag atacgacttg tgctgtttgt ttgaacttct
    38161 ggggtcgtac cgtccatgcg ctatcgcatt ttccttctct tttttttcgc tttgttgccg
    38221 acgtctttgg tgtgggcggc accagcgcaa cgggcgtttt ccgactggca ggtcacctgt
    38281 aataaccaaa atttctgcgt ggcgcgtaat acgggcgatc ataatggact ggtgatgacc
    38341 ctgagccgca gcgccggggc gcataccgat gccgttttac gtattgagcg cggcggattg
    38401 aagtcgccgg aggcgtcaga aggggagata gcgccacggc tgctgttaga tggcgagccg
    38461 ttggcgttaa gtggtgacaa gtggcggatt tcaccatggt tattagtaac cgatgatacg
    38521 gcaaccatca ccgcgttttt gcagatgatt caggaaggga aggcaatcac cttacgcgat
    38581 ggcgatcaga ccatttctct gagtggctta aaagcagcgt tgttgtttat tgatgctcag
    38641 caaaagcgcg ttggcagtga aaccgcgtgg atcaagaaag gggacgaacc gccgctcagc
    38701 gtaccgcccg cgcctgcgct gaaagaggtc gcggtggtta acccaacgcc gacgccactc
    38761 tcactcgaag aacgcaacga tttgctggat tatggaaact ggcggatgaa tggtctgcgc
    38821 tgctcgcttg atccattgcg tcgtgaggtg aatgtcactg cgctgactga tgataaagcg
    38881 ctgatgatga ttagctgtga ggcaggggcc tataacacca ttgatttggc atggattgtg
    38941 tcgcgtaaaa agccactagc ttcgcgcccg gttcggttgc gtttgccgtt caacaacggt
    39001 caggagacga atgaactgga actgatgaac gcaacatttg atgagaaatc gcgtgaactg
    39061 gtgaccttag cgaaagggcg cggattaagc gattgtggca ttcaggcgcg ctggcgcttt
    39121 gacggtcagc gttttcgcct ggtgcgttac gccgcagaac ccacctgcga taactggcat
    39181 gggccagacg cctggcccac gttgtggatc acccgttaaa caaaatggcg ggacatgtag
    39241 gccggataag acgctgtagg ccggataaga cgcgtcagcg tcgcatccgg caatcagcat
    39301 ccggcaatca ccatcaggca cctttcactc ccagcacctt atgcgctttt gccacaatat
    39361 tctcggcggt aaagccaaag aacgggaaca gcttatccgc cggagcagat tccccgtaac
    39421 ccgtcatccc gacaattgcc cctttcagac caacatactt gtaccagtaa tcggcaatac
    39481 ctgcttccac cgccacgcga gccgcaacgt tagaaggcaa caccgactcc cgatattcct
    39541 catcctgggc gtcgaaaata tcggtcgagg gcagggaaac tacgcgtaca ttgcgacctt
    39601 ctcctgctaa tttctctgcc gcttgcaggg taatttccat ctctgaaccg gtggcaatca
    39661 gaataatatc tggcttaccg ccgctgtctt tcagcacata accgccacga gcaatctctt
    39721 taacctgatc cggcgtacgt tccacctggg ccagattctg ccttgagagg atcagtgccg
    39781 tcggtccgtt gtggcgctca accgccagct tccagcccac cgccgcttcc acctgatcgc
    39841 acggtcgcca ggtgctgaaa tttggcgtta agcgcaggct ggccagttgc tcaacagcct
    39901 ggtgcgtcgg accatcttcg cccaggccaa ttgagtcgtg ggtataaacc ataatctgcc
    39961 gcgctttcat cagtgccgcc atccgcgcgg cgttacgggc gtattcaaca aacatcagga
    40021 atgtcgcggt atacggcaca aagccgccgt ggtgcgcgat gccgttggcg atagcggtca
    40081 tgccaaattc acgcaccccg tagtgaatgt agttgcccgc tggatcttcc ttcagcgaaa
    40141 cagaaccttt ccagatggtc aggttgctgg gagccagatc cgccgaaccg ccgagcaact
    40201 caggcagcat cggcccgtaa gcgttaagcg tattttgcga agccttacgg gtagcgattt
    40261 tcgccggatt tgcctgtaac tcattgatat atttctgagt cgttttctcc cagtccttcg
    40321 gtaaaccacc gctcatccgt cgggtaaact cttctgccag ttgcggatga gcctttttat
    40381 aggcggcaaa cttctcattc cagctctgct gcgctttttc gcctttttca cgggcatccc
    40441 aggcgtgata aatctcttta gggatctcaa atggcggatg gtgccagccc agtttttgcc
    40501 gtgccagcgc cacttcttct tcccccagtg gtgcgccgtg cgcctcttcc ttacctgctt
    40561 tattcggcga accaaagcca atcaccgtac ggcagataat cagcgacggc ttatctttca
    40621 cgctttgcgc ttcaaggatc gcttccttca ccgcctgcgg atcgtgaccg tcgatttcat
    40681 ggatcacatg ccagtgatag gcttcaaaac gttttgccgt atcgtcggta aaccagcctt
    40741 ctgtttcacc gtcgatggaa ataccgttgt gatcgtaaaa accaatcagc ttgcccagtc
    40801 ccagcgtgcc tgccagcgaa cagacttcgt gggaaatacc ttccatcagg cagccgtcgc
    40861 ccataaacac ataggtgaag tgatcgacga tctcatggtc tggctggtta aactgcgccg
    40921 ccagtgtacg ctctgctatc gccagcccga cggcgttcgc caaaccttgt ccaagcgggc
    40981 cggtggtggt ttcaacgcct ggcgtatagc caatctccgg gtggcctggg gttttcgaat
    41041 gcaactgacg gaagttcttc agttcttcca gcggcaggtc gtaaccggtc agatgtagca
    41101 aactgtagag cagcatcgac gcgtgaccgt tggaaagaat aaagcggtcg cgatcatacc
    41161 aggttgggtc ggtagggtta tgtttaagaa aatcgttcca cagcacttcg gcaatatcag
    41221 ccatgcccat cggcgcgccg ggatgaccag agttggcttt ttgtaccgca tccatactga
    41281 gtgcgcgaat cgcattggca aggtcttttc gggacatata acactccgtg gctggtttat
    41341 agtttggcgg caagaagatc ttccagtttg cgttgatcaa cggcgaacag acgaatgcct
    41401 tcagacagtt tttctaccgc catcgcatcc tgattgtgct cccaacggaa ctccgcttcg
    41461 ctcatgggag ctgggcgtgg gaacgtctga gaaggtggga ttaatttacg taccactggc
    41521 gaaacttttt cctgcagctc cttcagcaaa ttcggtgcga tagtcagtcg atcgcagccg
    41581 gttaaggcga ggatttgttc ggtgcgacgg aagctcgcgc ccatcacaat ggtttcatag
    41641 tggtgttgct tatagtagtc gtagatattg cgcaccgatt taacgcccgg atcttcttcc
    41701 accacatacg ggtccatcgg cttgcgtgcc tgataccagt cataaatacg cccgacaaac
    41761 ggcgaaatca gaaaaacgcc tgcttccgca caggcccgtg cctgtgcaaa agaaaacagc
    41821 agcgtcaggt tgcagttaat accttctttt tccagctctt ctgccgcgcg aattccttcc
    41881 caggtcgaag ccagcttgat cagaatgcgt gatttctcaa cgccttgttg ctgatacaag
    41941 tccaccagat ggcgcgcctt ctcaatactc ttttctttat caaaagagag gcgtgcatca
    42001 acttctgttg acacgcgacc gggtacgatt ttgaggattt cagcaccgaa attgaccgcc
    42061 agtttgtcac acgctgcgac cacctgttgt tcctgggtct tgccattttt tttaccccag
    42121 gcgatagcat cgtctattaa atgctcatat tgtgataatc cggcagcctt gagtaacagc
    42181 gaaggattgg tggtggcatc ctggggatga taatggcgaa tggactcaat atcgccgctg
    42241 tctgccacga cagtggtgaa ctgtttgatg ccgtctaact cgttcatagg aaatactcct
    42301 tgaaaagtaa agtgttagat gagtgcgtta attcacactt ctgagaaatt tcgctaaacg
    42361 catcaaaaaa gcatagcaga caggcatggt attgctggat taagcaggta acatcagtgt
    42421 tataggatta ttaccaaaac attatattaa tggtgagagt ttggacttgc tcaaagtctg
    42481 tagactccgg caaggtaata atgtgcgcca cgttgtgggc agggggtgtt gcccacacac
    42541 tttatttgtg aacgttacgt gaaaggaaca accaaatgga tgaccagtta aaacaaagtg
    42601 cacttgattt ccatgaattt ccagttccag ggaaaatcca ggtttctcca accaagcctc
    42661 tggcaacgca gcgcgatctg gcgctggcct actcaccagg cgttgccgca ccttgtcttg
    42721 aaatcgaaaa agacccgtta aaagcctaca aatataccgc gcgcggtaac ctggtggcgg
    42781 tgatctctaa cggtacggcg gtgctggggt taggcaacat tggcgcgctg gctggcaaac
    42841 cggtgatgga aggcaagggc gttctgttta agaaattcgc cgggattgat gtatttgaca
    42901 ttgaagttga tgaactcgac ccggacaaat ttattgaagt cgtcgctgcg ctcgaaccga
    42961 ccttcggcgg aatcaacctc gaagacatta aagcgccaga atgtttctat attgaacaga
    43021 aactgcgcga gcggatgaat attccggtat tccacgacga tcagcacggc acggcaatca
    43081 tcagcactgc cgccatcctc aacggcttgc gcgtggtgga gaaaaacatc tctgacgtgc
    43141 ggatggtggt ttccggcgcg ggtgccgcag caatcgcctg tatgaacctg ctggtcgcgc
    43201 tgggtctgca aaaacataac atcgtggttt gcgattcaaa aggcgttatc tatcagggcc
    43261 gtgagccaaa tatggcggaa accaaagccg catatgcggt ggtggatgac ggcaaacgta
    43321 ccctcgatga tgtgattgaa ggtgcggata ttttcctggg ctgttccggc ccgaaagtgc
    43381 tgacccagga aatggtgaag aaaatggccc gtgcgccaat gatcctggcg ctggcgaacc
    43441 cggaaccgga aattctgccg ccgctggcga aagaagtgcg tccggatgcc atcatctgta
    43501 ccggccgttc cgactacccg aaccaggtga acaacgtcct ttgcttcccg ttcatcttcc
    43561 gtggcgcgct ggacgttggc gcaaccgcca tcaacgaaga gatgaaactg gcggcggtac
    43621 gcgcgattgc agaactggcc catgcggaac agagcgaagt ggtggcttca gcgtatggcg
    43681 atcaggatct gagttttggt ccggaataca tcattccaaa accgtttgat ccgcgcttga
    43741 tcgttaagat cgcacctgcg gtggcgaaag cggcgatgga gtcgggcgtg gcgacgcgtc
    43801 cgattgctga tttcgacgtc tacatcgaca agctgactga gttcgtttac aaaaccaacc
    43861 tgtttatgaa gccgattttc tcccaggctc gcaaagcgcc gaagcgcgtt gttctgccag
    43921 aaggggaaga ggcgcgcgtt ctgcatgcca ctcaggaact ggtaacgctg ggactggcga
    43981 aaccgatcct tatcggtcgt ccgaacgtga tcgaaatgcg cattcagaaa ctgggcttgc
    44041 agatcaaagc gggcgttgat tttgagatcg tcaataacga atccgatccg cgctttaaag
    44101 agtactggac cgaatacttc cagatcatga agcgtcgcgg tgtcactcag gaacaggcgc
    44161 agcgggcgct gatcagtaac ccgacagtga tcggcgcgat catggttcag cgtggggaag
    44221 ccgatgcaat gatctgcggt acggtgggcg attatcatga acattttagc gtggtgaaaa
    44281 atgtctttgg ttatcgcgat ggcgttcaca ccgcaggtgc aatgaacgcg ctgctgctgc
    44341 cgagtggtaa cacctttatt gccgatacct acgtcaatga cgaaccagac gcagaagagc
    44401 tggcggagat caccttgatg gcggcagaaa ctgtccgtcg ttttggtatt gagccgcgcg
    44461 ttgctctgtt gtcgcactcc aactttggtt cttctgactg cccgtcgtcg agcaaaatgc
    44521 gtcaggcgct ggaactggtc agggatcgtg cgccagatct gatgattgat ggtgaaatgc
    44581 acggcgatgc agcgctggtg gaagcgattc gcaacgaccg tatgccggac agccctttga
    44641 aaggttctgc caatattctg gtgatgccga acatggaagc tgcccgcatt agttacaact
    44701 tactgcgcgt ttccagctcg gaaggtgtga ctgtcggtcc ggtgctgatg ggcgtggcga
    44761 aaccggttca cgtgttaacg ccgatcgcat cggtgcgtcg tatcgtcaac atggtggcgc
    44821 tggccgtggt tgaagcgcaa acccaaccgc tgtaattctt ttttaactct cacgcttacc
    44881 ctgaatattc agggtaagcg gttt
//
LOCUS       DAOFQD010000025        41511 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.025, whole genome shotgun sequence.
ACCESSION   DAOFQD010000025 DAOFQD010000000
VERSION     DAOFQD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.025"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..314)
                     /locus_tag="QBF46_001440"
     CDS             complement(<1..314)
                     /locus_tag="QBF46_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDQ6673637.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPV"
     gene            577..663
                     /gene="azuC"
                     /locus_tag="QBF46_001441"
     CDS             577..663
                     /gene="azuC"
                     /locus_tag="QBF46_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDQ6673638.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            778..1029
                     /locus_tag="QBF46_001442"
     CDS             778..1029
                     /locus_tag="QBF46_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDQ6673639.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1108..1611)
                     /locus_tag="QBF46_001443"
     CDS             complement(1108..1611)
                     /locus_tag="QBF46_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDQ6673640.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            2408..3397
                     /gene="araF"
                     /locus_tag="QBF46_001444"
     CDS             2408..3397
                     /gene="araF"
                     /locus_tag="QBF46_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDQ6673641.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVVKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3467..4981
                     /gene="araG"
                     /locus_tag="QBF46_001445"
     CDS             3467..4981
                     /gene="araG"
                     /locus_tag="QBF46_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDQ6673642.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            4996..5982
                     /gene="araH"
                     /locus_tag="QBF46_001446"
     CDS             4996..5982
                     /gene="araH"
                     /locus_tag="QBF46_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDQ6673643.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            6149..6949
                     /gene="otsB"
                     /locus_tag="QBF46_001447"
     CDS             6149..6949
                     /gene="otsB"
                     /locus_tag="QBF46_001447"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDQ6673644.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            6924..8348
                     /gene="otsA"
                     /locus_tag="QBF46_001448"
     CDS             6924..8348
                     /gene="otsA"
                     /locus_tag="QBF46_001448"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDQ6673645.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(8355..8783)
                     /gene="uspC"
                     /locus_tag="QBF46_001449"
     CDS             complement(8355..8783)
                     /gene="uspC"
                     /locus_tag="QBF46_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDQ6673646.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            9562..9911
                     /gene="flhD"
                     /locus_tag="QBF46_001450"
                     /pseudo
     CDS             9562..9911
                     /gene="flhD"
                     /locus_tag="QBF46_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
     gene            9914..10492
                     /gene="flhC"
                     /locus_tag="QBF46_001451"
     CDS             9914..10492
                     /gene="flhC"
                     /locus_tag="QBF46_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDQ6673647.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            10619..11506
                     /gene="motA"
                     /locus_tag="QBF46_001452"
     CDS             10619..11506
                     /gene="motA"
                     /locus_tag="QBF46_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDQ6673648.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            11503..12429
                     /gene="motB"
                     /locus_tag="QBF46_001453"
     CDS             11503..12429
                     /gene="motB"
                     /locus_tag="QBF46_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDQ6673649.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            12434..14398
                     /gene="cheA"
                     /locus_tag="QBF46_001454"
                     /pseudo
     CDS             12434..14398
                     /gene="cheA"
                     /locus_tag="QBF46_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
     gene            14419..14922
                     /gene="cheW"
                     /locus_tag="QBF46_001455"
     CDS             14419..14922
                     /gene="cheW"
                     /locus_tag="QBF46_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDQ6673650.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            15067..16728
                     /gene="tar"
                     /locus_tag="QBF46_001456"
     CDS             15067..16728
                     /gene="tar"
                     /locus_tag="QBF46_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDQ6673651.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLRKQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            16774..18375
                     /gene="tap"
                     /locus_tag="QBF46_001457"
     CDS             16774..18375
                     /gene="tap"
                     /locus_tag="QBF46_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDQ6673652.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESVQLQIAPVVS"
     gene            18394..19254
                     /gene="cheR"
                     /locus_tag="QBF46_001458"
     CDS             18394..19254
                     /gene="cheR"
                     /locus_tag="QBF46_001458"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDQ6673653.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            19257..20306
                     /gene="cheB"
                     /locus_tag="QBF46_001459"
     CDS             19257..20306
                     /gene="cheB"
                     /locus_tag="QBF46_001459"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDQ6673654.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            20321..20710
                     /gene="cheY"
                     /locus_tag="QBF46_001460"
     CDS             20321..20710
                     /gene="cheY"
                     /locus_tag="QBF46_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDQ6673655.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            20721..21365
                     /gene="cheZ"
                     /locus_tag="QBF46_001461"
     CDS             20721..21365
                     /gene="cheZ"
                     /locus_tag="QBF46_001461"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDQ6673656.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            21567..22715
                     /gene="flhB"
                     /locus_tag="QBF46_001462"
     CDS             21567..22715
                     /gene="flhB"
                     /locus_tag="QBF46_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDQ6673657.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            22708..24786
                     /gene="flhA"
                     /locus_tag="QBF46_001463"
     CDS             22708..24786
                     /gene="flhA"
                     /locus_tag="QBF46_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDQ6673658.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            24786..25178
                     /gene="flhe"
                     /locus_tag="QBF46_001464"
     CDS             24786..25178
                     /gene="flhe"
                     /locus_tag="QBF46_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDQ6673659.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(25299..25787)
                     /locus_tag="QBF46_001465"
     CDS             complement(25299..25787)
                     /locus_tag="QBF46_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDQ6673660.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DVNVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(25964..27697)
                     /gene="argS"
                     /locus_tag="QBF46_001466"
     CDS             complement(25964..27697)
                     /gene="argS"
                     /locus_tag="QBF46_001466"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDQ6673661.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            27913..28479
                     /locus_tag="QBF46_001467"
     CDS             27913..28479
                     /locus_tag="QBF46_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDQ6673662.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            28493..29239
                     /gene="cutC"
                     /locus_tag="QBF46_001468"
     CDS             28493..29239
                     /gene="cutC"
                     /locus_tag="QBF46_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDQ6673663.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            29627..30727
                     /locus_tag="QBF46_001469"
     CDS             29627..30727
                     /locus_tag="QBF46_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDQ6673664.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQTATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LAVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            30752..33181
                     /gene="torZ"
                     /locus_tag="QBF46_001470"
     CDS             30752..33181
                     /gene="torZ"
                     /locus_tag="QBF46_001470"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDQ6673665.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANSTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQVVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(33346..34317)
                     /gene="cmoB"
                     /locus_tag="QBF46_001471"
     CDS             complement(33346..34317)
                     /gene="cmoB"
                     /locus_tag="QBF46_001471"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDQ6673666.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEGPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(34314..35057)
                     /gene="cmoA"
                     /locus_tag="QBF46_001472"
     CDS             complement(34314..35057)
                     /gene="cmoA"
                     /locus_tag="QBF46_001472"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDQ6673667.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLDPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HNAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(35098..35493)
                     /locus_tag="QBF46_001473"
     CDS             complement(35098..35493)
                     /locus_tag="QBF46_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDQ6673668.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(35546..36364)
                     /locus_tag="QBF46_001474"
     CDS             complement(35546..36364)
                     /locus_tag="QBF46_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDQ6673669.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWNFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            complement(36361..36927)
                     /locus_tag="QBF46_001475"
     CDS             complement(36361..36927)
                     /locus_tag="QBF46_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDQ6673670.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIVRVRSVEEILHAL"
     gene            37237..39009
                     /gene="aspS"
                     /locus_tag="QBF46_001476"
     CDS             37237..39009
                     /gene="aspS"
                     /locus_tag="QBF46_001476"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDQ6673671.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRTDVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILNRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            39127..39579
                     /gene="nudB"
                     /locus_tag="QBF46_001477"
     CDS             39127..39579
                     /gene="nudB"
                     /locus_tag="QBF46_001477"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDQ6673672.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            39608..40348
                     /locus_tag="QBF46_001478"
     CDS             39608..40348
                     /locus_tag="QBF46_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ6673673.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            40383..40904
                     /gene="ruvC"
                     /locus_tag="QBF46_001479"
     CDS             40383..40904
                     /gene="ruvC"
                     /locus_tag="QBF46_001479"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDQ6673674.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(40906..41508)
                     /locus_tag="QBF46_001480"
     CDS             complement(40906..41508)
                     /locus_tag="QBF46_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDQ6673675.1"
                     /translation="MNINYPAEYEIGDIAFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGAETPQPELTEAV"
BASE COUNT        10465 a         9950 c        11084 g        10012 t
ORIGIN      
        1 accgggaagg cggaatgatg gcactgatat gacaaagtga attccgtatt caaacataag
       61 gaacccgcaa aaaggctaca gttgcctact ggctgaccaa aggggaccgc gtgggtatag
      121 ccttcctgct ggcatgcgcg atcggcgatc ccgcgactga cataacgatc ggtatgggca
      181 tgctgcaaaa aagcctggtc ataaaccaac cgtacaatac ctgttgccgc gtcaacttcg
      241 ctcacatggg cctggcgagt aaccgtacac cccgccagcg ttattaatga aagagtcaga
      301 atcagaagtc tcatggtcca gccagaagag taagagaaag cactatttta cgcgttacag
      361 cgctgcgaca atggccggaa aagacgcctg gcaggcgcta tttcttgcca tctgtaggtg
      421 tcagaaatag gggggctgtt ttttgattat catttttata acattaaagg ggtatttatg
      481 tgatttgtat cacataaaga ttgatttttg tgatggttgt catattataa gcgcaaagac
      541 aataacacct gtataacaaa tggtcggagt gccgcgatga aactgcgcaa aatcctgaaa
      601 agtatgttca ataactattg caagacgttc aaagacgtac cgccaggcaa tatgttccga
      661 taataaaaaa cctgctccgg caggtttttt tgtgtcctga tgacggtgga tgtgactatc
      721 gctgaagatg tgaagccgct actataacgg ctttcaaaat gctatcagga gtttacgatg
      781 tcccagccgc tgaatgccga tcaggaactg gtttctgatg tggttgcttg ccagttggtt
      841 atcaaacaaa ttctggatgt tctcgatgta atcgcgcccg ttgaagttcg tgaaaaaatg
      901 tccagtcagc tgaagaatat cgactttact aaccatcccg ccgccgcaga cccggtcacc
      961 atgcgggcaa tccaaaaagc catcgcgctt attgaactca agtttacccc acagggtgaa
     1021 tcccattaaa aaaaccgctg cttcaaacag atgaggcagc ggtttctcaa aaccttcatc
     1081 ggcgcaatgc attagcgccg atgatgatca atgcagctgg tgtgacacga cattcagaac
     1141 atgttggtcg gtctgcacag ggcacatacc cgcaagtttc gcactgcgca cttcatcaag
     1201 aatggtttgc agcaacagac catcatgctg ctgttctttt tccagatcgc gcaggaaatt
     1261 gacggttgaa tcatcattca gttctttcgc ttcatcggct aactgtgcca acgtactaga
     1321 acgttgctcg tattcttcca tcgttttttg gaacagttct tccagagagt tcagtttttc
     1381 accgggaaca tcaatggctt taacgatggg ggtagcgccg acactcttca taaagttaaa
     1441 catgcgcatc atttgggtca cattactctg tgcctgggcg cgaaggaaag tggcggtgcc
     1501 gttcagactc tgttcagaac accagttact caggtgaagg tagagattgg atgcgtaaaa
     1561 ctcgcggttc atttgagagt tgagtttgag aagcattcca gcggttgcca tacctaatat
     1621 ccttatatcc agaagtggag aggtgcaaga taaatcaaaa caccataatt actgtttgca
     1681 gaataattcc tttctgccgg tccgaaatgt gatcgtggag tcaattctga cgatgatctg
     1741 aaataagaat aacgtctgaa cgggtataag aaaatgctta aatcatcaag tttatatatt
     1801 tttacatttc attgatgaaa tcaatgtaac tgcaatgaat tttaatgata gcgctttttg
     1861 tatcttgttg atattaatca atgaatttga attcatgatg aagtgatgat tttataaaac
     1921 gttttttcat ttttgcgaga tggctctcat tatacgtgtt ctgttaatta atgagcacag
     1981 tgataattaa tggcgaaatg aatgacgtgc attttccaca tctttgagtt gcggttatta
     2041 caccatttca aaaactcaac gccaggtaat gcggcctatt gactggttaa aaagaagaca
     2101 tcccgcatgg gtaccaaaga caacaaggat ttccaggcta atcttatgga ttaatctgct
     2161 gtgcattcga caatttgtct gacaaattgg ctttccctta tgtcttttcc cgctaaattt
     2221 atgcacgttc tcactgtaat tctgcgatgt gatattgctc tcctatggag aattaatttc
     2281 tcgctaaaac tatgtcaaca cggtcactta tcttttagtt aaaaggtaat gctttgtttt
     2341 ccgattaatt taacgaatgt cattcgtttt tgccctacac aaaacgacac taaagctgga
     2401 gagaaccatg cacaaattta ctaaagccct ggcagccatt ggtctggcag ccgttatgtc
     2461 acaatccgct atggcggaga acctgaagct cggttttctg gtgaagcaac cggaagagcc
     2521 gtggttccag accgaatgga agtttgccga taaagccggg aaggatttag ggtttgaggt
     2581 tattaagatt gccgtgccgg atggcgaaaa aacattgaac gcgatcgaca gcctggctgc
     2641 cagtggcgca aaaggtttcg ttatttgtac tccggacccc aaactcggct ctgccatcgt
     2701 cgcgaaagcg cgtggctacg atatgaaagt cattgccgtg gatgaccagt ttgttaacgc
     2761 caaaggtaag ccaatggata ccgttccgct ggtgatgatg gcggcgacta aaattggcga
     2821 acgtcagggc caggaactgt ataaagagat gcagaaacgt ggctgggatg tcaaagaaag
     2881 cgcggtgatg gcgattaccg ccaacgaact ggataccgcc cgccgccgta ctacgggatc
     2941 tatggatgcg ctgaaagcgg ccggattccc ggaaaaacaa atttatcagg tacctaccaa
     3001 atctaacgac atcccggggg catttgacgc tgccaactca atgctggttc aacatccgga
     3061 agttaaacat tggctgatcg tcggtatgaa cgacagcacc gtgctgggcg gcgtacgcgc
     3121 gacggaaggt cagggcttta aagcggccga tatcatcggc attggcatta acggtgtgga
     3181 tgcggtgagc gaactgtcta aagcacaggc aaccggcttc tacggttccc tgctgccaag
     3241 cccggacgta catggctata aatccagcga aatgctttac aactgggtag taaaagacgt
     3301 tgaaccgcca aaatttaccg aagttaccga cgtggtactg atcacgcgtg acaactttaa
     3361 agaagaactg gagaaaaaag gtttaggcgg taagtaattt gccggaaaaa ttcccctctg
     3421 catgatgcag agggggtgtg aacgaccagt gattcacgga gacgttatgc aacagtctac
     3481 cccgtatctc tcatttcgcg gcatcggtaa aacgtttccc ggcgttaagg cgctgacgga
     3541 tattagtttt gactgctatg ccggtcaggt tcatgcgttg atgggtgaaa atggcgcagg
     3601 aaaatcaact ctcttaaaaa tcctcagcgg caactatgcg ccaaccacgg gttctgtagt
     3661 gattaatggg caggaaatgt ccttttccga cacgaccgca gcacttaacg cgggtgtggc
     3721 gattatttac caggaactac atctcgtgcc ggaaatgacc gtcgcggaaa acatctatct
     3781 cggccagctg ccgcataaag gcggcattgt gaatcgctca ttgctgaatt atgaggcggg
     3841 tttacaactt aaacatcttg gtatggatat tgacccggac acgccgctga aatatctctc
     3901 cattggtcag tggcagatgg ttgaaatcgc caaggcgctg gcgcgtaacg ctaaaattat
     3961 cgcctttgat gagccaacca gctccctttc tgcccgcgaa atcgacaatc ttttccgcgt
     4021 tattcgtgaa ctgcgaaaag aggggcgggt gatcttatac gtttctcacc gtatggaaga
     4081 aatatttgcc ctcagcgatg ccatcactgt ctttaaagat ggacgttatg tcaaaacctt
     4141 taccgatatg cagcaggttg accacgacgc gctggtgcag gcgatggtcg ggcgcgacat
     4201 tggcgatatc tacggctggc aaccgcgtag ttatggcgag gagcgcctgc gtcttgatgc
     4261 tgtgaaagca ccaggcgtgc gtacgccaat aagtctggcg gttcgcagtg gtgaaattgt
     4321 tgggctgttt ggtctggtag gggcggggcg tagcgaatta atgaaaggca tgtttggcgg
     4381 gacgcaaatc accgccggtc aggtttatat cgaccaacag ccgatcgata ttcgtaaacc
     4441 gagccacgcc attgccgcag gcatgatgct ctgcccggaa gatcgcaaag cggaaggcat
     4501 tattcccgtg cactccgttc gcgacaatat caacatcagt gccagacgta aacatgtgct
     4561 cggcggttgt gtaatcaaca acggttggga agaaaacaat gccgatcacc acattcgttc
     4621 gctcaacatc aaaacgccgg gcgcggagca actgatcatg aatctctcag gcggaaatca
     4681 gcaaaaagcc attctgggcc gctggttatc ggaagagatg aaggtcattt tgctggatga
     4741 acctacgcgc ggcattgatg ttggcgctaa gcacgaaata tataacgtaa tttatgcgct
     4801 ggcggcgcag ggcgtggcgg tgctgtttgc ctccagcgac ttacctgaag tcctcggcgt
     4861 tgccgaccgg attgtggtga tgcgggaagg tgaaatcgcc ggtgaattgt tacacgagca
     4921 ggcagatgag cgtcaggcac tgagccttgc gatgcctaaa gtcagccagg ctgttgcctg
     4981 agtaaggaga gtatgatgtc ttctgtttct acatcggggt ctggcgcacc taagtcgtca
     5041 ttcagcttcg ggcgtatctg ggatcagtac ggcatgctgg tggtgtttgc ggtgctcttt
     5101 atcgcctgtg ccatttttgt cccaaatttt gccaccttca ttaatatgaa agggttgggc
     5161 ctggcaattt ccatgtcggg gatggtggct tgtggcatgt tgttctgcct cgcttccggt
     5221 gactttgacc tttctgtcgc ctccgtaatt gcctgtgcgg gtgtcaccac ggcggtggtt
     5281 attaacctga ctgaaagcct gtggattggc gtggcagcgg ggttgttgct gggcgttctc
     5341 tgtggcctgg tcaatggctt tgttatcgcc aaactgaaaa taaatgctct gatcacgaca
     5401 ttggcaacga tgcagattgt tcgaggtctg gcgtacatca tttcagacgg taaagcggtc
     5461 ggtatcgaag atgaaagctt ctttgccctt ggttacgcca actggttcgg tctgcctgcg
     5521 ccaatctggc tcaccgtcgc gtgtctgatt atctttggtt tgctgctgaa taaaaccacc
     5581 tttggtcgta acaccctggc gattggcggg aacgaagagg ctgcgcgtct ggcgggtgta
     5641 ccggttgttc gcaccaaaat tattatcttt gttctctctg gcctggtatc tgcgatagcc
     5701 ggaattattc tggcttcacg tatgactagt gggcagccaa tgacgtcaat tggttatgag
     5761 cttattgtta tctccgcctg cgttttaggt ggcgtttctc tgaaaggtgg catcggaaaa
     5821 atctcatatg tggtggcggg tatcttaatt ttaggcaccg tggaaaacgc catgaacctg
     5881 cttaatattt ctcctttcgc gcagtacgtg gttcgcggct taatcctgct ggcagcggtg
     5941 atcttcgacc gttacaagca aaaagcgaaa cgcactgtct gatgcttttt tccacaacaa
     6001 tttaacgttt tttcccacca cagccagccg ccacaacggt tggctgttct tcattgcaaa
     6061 tggcgacccc cgtcacactg tctatactta catgtctgta aagcgcgttc tgcgcaaaac
     6121 aataagaaaa gagaaggagg agagccgggt gacagaaccg ttaaccgaaa cccctgaact
     6181 atccgcgaaa tatgcctggt tttttgatct tgatggaacg ctggcggaaa tcaaaccgca
     6241 tcccgatcag gtcgtcgtgc ctgacaatat tctgcaagga ctacagctac tggcaaccgc
     6301 aagtgatggt gcattggcat tgatatcagg gcgctcaatg gtggagcttg acgcactggc
     6361 aaaaccttat cgcttcccgt tagcgggcgt gcatggggcg gagcgccgtg acatcaatgg
     6421 taaaacacat atcgttcatc tgccggatgc gattgcgcgt gatattagcg tgcaactgca
     6481 tacagtcatc gctcagtatc ccggcgcgga gctggaggcg aaagggatgg cttttgcgct
     6541 gcattatcgt caggctccgc agcatgaaga cgcattaatg acattagcgc aacgtattac
     6601 tcagatctgg ccacaaatgg cgttacagca gggaaagtgt gttgtcgaga tcaaaccgag
     6661 aggtaccagt aaaggtgagg caattgcagc ttttatgcag gaagctccct ttatcgggcg
     6721 aacgcccgta tttctgggcg atgatttaac cgatgaatct ggcttcgcag tcgttaaccg
     6781 actgggcgga atgtcagtaa aaattggcac aggtgcaact caggcatcat ggcgactggc
     6841 gggtgtgccg gatgtctgga gctggcttga aatgataacc accgcattac aacaaaaaag
     6901 agaaaataac aggagtgatg actatgagtc gtttagtcgt agtatctaac cggattgcac
     6961 caccagacga gcacgccgcc agtgccggtg gccttgccgt tggcatactg ggggcactga
     7021 aagccgcagg cggactgtgg tttggctgga gtggtgaaac agggaatgag gatcagccgc
     7081 taaaaaaggt gaaaaaaggt aacattacgt gggcctcttt taacctcagc gaacaggacc
     7141 ttgacgaata ctacaaccaa ttctccaatg ccgttctctg gcccgctttt cattatcggc
     7201 tcgatctggt gcaatttcag cgtcctgcct gggacggcta tctacgcgta aatgcgttgc
     7261 tggcagataa attactgccg ctgttgcaag acgatgacat tatctggatc cacgattatc
     7321 acctgttgcc atttgcgcat gaattacgca aacggggagt gaataatcgc attggtttct
     7381 ttctgcatat tcctttcccg acaccggaaa tcttcaacgc gctgccgaca tatgacacct
     7441 tgcttgaaca gctttgtgat tatgatttgc tgggtttcca gacagaaaac gatcgtctgg
     7501 cgttcctgga ttgtctttct aacctgaccc gcgtcacgac acgtagcgca aaaagccata
     7561 cagcctgggg caaagcattt cgaacagaag tctacccgat cggcattgaa ccgaaagaaa
     7621 tagccaaaca ggctgccggg ccactgccgc caaaactggc gcaacttaaa gcggaactga
     7681 aaaacgtaca aaatatcttt tctgtcgaac ggctggatta ttccaaaggt ttgccagagc
     7741 gttttctcgc ctatgaagcg ttgctggaaa aatatccgca gcatcatggt aaaattcgtt
     7801 atacccagat tgcaccaacg tcgcgtggtg atgtgcaagc ctatcaggat attcgtcatc
     7861 agctcgaaaa tgaagctgga cgaattaatg gtaaatacgg gcaattaggc tggacgccgc
     7921 tttattattt gaatcagcat tttgaccgta aattactgat gaaaatattc cgctactctg
     7981 acgtgggctt agtgacgcca ctgcgtgacg ggatgaacct ggtagcaaaa gagtatgttg
     8041 ctgctcagga cccagccaac ccgggcgttc ttgttctttc gcaatttgcg ggagcggcaa
     8101 acgagttaac gtcggcgtta attgttaacc cctacgatcg tgacgaagtt gcagctgcgc
     8161 tggatcgtgc attgactatg tcgctggcgg aacgtatttc ccgtcatgca gaaatgctgg
     8221 acgttatcgt gaaaaacgat attaaccact ggcaggagtg cttcattagc gacctaaagc
     8281 agatagttcc gcgaagcgcg gaaagccagc agcgcgataa agttgctacc tttccaaagc
     8341 ttgcgtagga gcggttaatc acccgtaagt ggaactaaca gcacatcgac ctcacttgag
     8401 gcaatcactc ttttcgccga gcaggatgct cgcgaaaaga aactgtgatt gtgattaccg
     8461 caaatcacca aatcgaaatg atgtttgtga catacttcca gaatatgttc gcttaactca
     8521 ccgtaggcaa taaacgtctt gtcaacggga taccccgcat cctgaattaa cttatcaaga
     8581 aagctttgcg tttcttcttg catcacgctg cgtaaatctt caagcatcgg cgcggctaat
     8641 tgattgtaca tttccgggtc ggaagcgaga gtaattaaac tgatgtgtcc ctttactggc
     8701 ctggcgatag ataccgcttt tgccagcagt tgctgacttt ccggtgtaac cgcaacagcg
     8761 acaagaatat tgctatagct cataacctgt tccttattct gtgaacttca ggtgacatta
     8821 aagctttact cctaatggag aaacgacgca atcccaactc ggtcaaacag gtaaatatta
     8881 gtctgattat tagtaaagac aattaatcaa tcactcccgg tgatcatata atttcaaatg
     8941 taattataag aaaatagtgt actgattctc cgcattaaac aagtggttaa tatatgtaaa
     9001 acatttcatt aacattaagt tgattgttgc ctttctttgt atttaattag tttgttgtgc
     9061 ggtaagtgtc tgtttaaaaa tagcttatct ggtattgcat gaaagtgatt atttatagca
     9121 gatgattatt tatggtgagt tattttaact gtgcgcaaca tcccatttcg attattcctg
     9181 tttcattttt gcttgctagc gtagcgaaaa actttttaac agattgaaat acacccaaaa
     9241 caaaagtatg acttatacat ttatgttaag taattgagtg ttttgtgtga tctgcatcac
     9301 acattattga aaatcgcagc ccccctccgt tgtatgtgcg tgtagtgacg agtacagttg
     9361 cgtcgattta ggaaaaatct tagataagtg taaagacccc cttctatttg taaggactta
     9421 ttaaaccaaa aaggtggctc tgcttattgc agcttatcgc aactattcta atgctaatta
     9481 tttttaccgg ggcttcccgg cgacatcaca gggtgcggtg aaaccgcata aaaataaagt
     9541 tggttattct gggtgggaat aatgcatacc tccgagttgc tgaaacacat ttatgacatc
     9601 aacttgtcat atttactact tgcaagcgtc tgattgttca ggacaaagcg tccgctatgt
     9661 ttcgtctcgg cataaatgaa gaaatggcga caacattagc ggcactgact cttccgcaaa
     9721 tggttaagct ggcagaaacc aatcagctgg tttgtcactt ccgttttgac agccaccaga
     9781 cgattactca gttgacgcaa gattcccgcg ttgacgatct ccagcaaatt cataccggca
     9841 tcatgctctc aacacgcttg ctgaatgatg ttaatcagcc tgaagaagcg ctgcgcaaga
     9901 aaagggcctg atcatgagtg aaaaaagcat tgttcaggaa gcgcgggata ttcagctggc
     9961 aatggaattg atcaccctgg gcgctcgttt gcagatgctg gaaagcgaaa cacagttaag
    10021 tcgcggacgc ctgataaaac tttataaaga actgcgcgga agcccaccgc cgaaaggcat
    10081 gctgccattc tcaaccgact ggtttatgac ctgggaacaa aacgttcatg cttcgatgtt
    10141 ctgtaatgca tggcagtttt tactgaaaac cggtttgtgt aatggcgtcg atgcggtgat
    10201 caaagcctac cgtttatacc ttgaacagtg cccacaagca gaagaaggac cactgctggc
    10261 attaactcgt gcctggacat tggtgcggtt tgttgaaagt ggattactgc aactttccag
    10321 ctgcaactgc tgcggcggca attttattac ccacgctcac cagcctgttg gcagctttgc
    10381 ctgcagctta tgtcaaccgc catcccgggc agtaaaaaga cgtaaacttt cccagaatcc
    10441 tgccgatatt atcccacaac tgctggatga acagagagta caggctgttt aactgatacg
    10501 gtgaggcgca acattccagc agcggtaacg acgtaccgct gctttttttt gccccaatcg
    10561 cgcgttaacg cctgacgact gaacatcctg tcatggtcaa cagtggaagg atgatgtcgt
    10621 gcttatctta ttaggttacc tggttgttct cggtacagtt ttcggcggtt atttgatgac
    10681 cggtggaagc cttggagcac tctatcaacc cgctgaactg gtgattattg ccggtgcagg
    10741 gattgggtcg tttatcgtcg gcaataatgg caaagcgatt aaaggcacgc tgaaggcgct
    10801 gccgttgctg tttcgtcgct ccaaatacac caaagcaatg tatatggatc tgctggctct
    10861 gctttatcgg ttgatggcaa aatcgcggca gatggggatg ttttcgctgg aacgtgatat
    10921 tgaaaatccc cgtgagagcg agatcttcgc cagctaccca cgcatcctcg cggatagcgt
    10981 catgcttgat tttatcgtcg attatctgcg cctgattatc agcggtcaca tgaacacctt
    11041 cgaaatcgaa gccctgatgg atgaagagat tgagacgcac gaaagcgagg cagaagtccc
    11101 ggcgaacagt ctggcgctgg tcggggactc acttccggcg tttggtattg tcgcggctgt
    11161 aatgggggtc gttcacgcgt taggttcagc cgatcgtcct gccgccgagc tgggtgcgct
    11221 tatcgcacat gcgatggtgg ggactttcct cggcatttta ttggcttacg gatttatttc
    11281 cccattagcg actgttttac gtcagaaaag cgccgaaacc agcaaaatga tgcagtgcgt
    11341 caaagtcact ctgctttcta atctgaacgg ttacgcaccg cctatcgccg ttgagtttgg
    11401 tcgcaaaacg ctctattcca gcgaacgtcc gtcgtttatt gaactggaag agcatgtgcg
    11461 tgcggtgaaa aatccgcaac aacagacgac aaccgaggaa gcatgaagaa tcaagcgcat
    11521 ccgattattg tcgtcaaacg acgcaaagcc aaaagccacg gggcagcaca tggatcgtgg
    11581 aagattgctt atgccgactt tatgactgcg atgatggcct tttttctggt gatgtggctg
    11641 atctccatct ccagcccaaa agagctgatt cagattgcgg agtacttccg gactccactg
    11701 gcgactgcgg ttacgggcgg cgatcgcatt tctaatagtg aaagcccaat tcccggcggt
    11761 ggtgatgatt acacccaaag ccagggggaa gtgaataagc agccgaacat cgaagagctg
    11821 aaaaaacgca tggagcaaag tcgattgcgg aaattgcggg gtgatctcga ccagttgata
    11881 gagtccgatc cgaaactgcg ggcgttacgt ccccatctca aaatcgatct ggtccaggaa
    11941 ggtctacgta ttcagatcat cgatagccag aatcgcccga tgtttagaac cggcagtgcc
    12001 gatgtcgaac cctatatgcg cgacattctg cgcgccattg cgcctgtact gaacggtatt
    12061 cccaaccgta ttagcctttc aggtcatacc gatgatttcc cctacgccag cggtgagaaa
    12121 ggatatagca actgggagct ttctgccgat cgggccaatg catcccgccg cgaactgatg
    12181 gtcggagggt tggatagcgg caaagtgtta cgtgtcgtcg gcatggcggc aacgatgcgc
    12241 ttaagcgatc gcggacctga tgatgccgtc aaccgtcgca tcagcctgct ggtactgaac
    12301 aaacaagccg aacaggccat tttgcatgaa aacgccgaaa gccagaatga gccagtaagc
    12361 gccctggaaa aacctgaggt tgcaccacag gtcagtgttc ccacaatgcc atcagccgaa
    12421 ccgaggtgac agcgtgagca tggatataag cgatttttat cagacatttt ttgatgaagc
    12481 ggacgaactg ttggctgaca tggagcagca tttgctggtt ttgcagccgg aagcgccaga
    12541 tgccgaacaa ttgaatgcca tctttcgggc tgcccactcg atcaaaggag gggcaggaac
    12601 ttttggcttc agcgttttgc aggaaaccac gcatctgatg gaaaacctgc tcgatgaagc
    12661 cagacgaggt gagatgcaac tcaacaccga cattatcaat ctgtttttgg aaacgaagga
    12721 catcatgcaa gaacagctcg acgcttataa acagtcgcaa gagccggatg ccgccagctt
    12781 cgattatatc tgccaggcct tgcgtcaact ggcattagaa gcgaaaggcg aaacgccatc
    12841 cgcagtgacc cgattaagtg tggttgccaa aagtgaaccg caagatgagc agagtcgcag
    12901 tcagtcgccg cgacgaatta tcctttcgcg cctgaaggcc ggggaagtcg acctgctgga
    12961 agaagaactg ggacatctga caacgttaac tgacgtggtg aaaggggcgg attagctctc
    13021 ggcaatatta ccgggcgaca tcgccgaaga tgacatcaca gcggtactct gttttgtgat
    13081 tgaagccgat cagattacct ttgaaacagt agaagtctcg ccaaaaatat ccaccccacc
    13141 agtgcttaaa ctggcagccg aacaagcgcc aaccggccgc gtggagcggg aaaaaacgac
    13201 gcgcagcaat gaatccacca gcatccgtgt agcggtagaa aaggttgatc aattaattaa
    13261 cctcgtcggc gagctggtta tcacccagtc catgcttgcc cagcgttcca gcgaactgga
    13321 cccggttaat catggtgatt tgataaccag catggggcag ttacaacgta acgcccgtga
    13381 tttgcaggaa tcagtgatgt cgattcgcat gatgccgatg gaatatgttt ttagtcgcta
    13441 tccccggctg gtgcgtgatc tggcgggaaa actcggcaag caggtagaac tgacgctggt
    13501 gggcagttct actgaactcg acaaaagtct gatagaacgc attatcgacc cgctgaccca
    13561 cctggtacgc aatagcctcg atcacggtat tgaactgcca gaaaaacggc tcgccgcagg
    13621 taaaaacagc gtcggaaatt taattctgtc tgccgaacat cagggcggca acatttgcat
    13681 tgaagtgacc gacgatgggg cggggctaaa ccgtgagcga attctggcaa aagcggcctc
    13741 gcaaggtttg actgtcagcg aaaacatgag cgacgacgaa gtcgcgatgc tgatatttgc
    13801 acctggcttc tccacggcag agcaggtcac cgacgtctcc gggcgcggcg tcggcatgga
    13861 cgtcgttaaa cgtaatatcc aggagatggg cggtcatgtc gaaatccagt cgaagcaggg
    13921 tactggcact acgatccgca ttttactgcc gctgacgctg gccatcctcg acggcatgtc
    13981 cgtacgcgtt gcggatgaag ttttcattct gccgctgaat gctgttatgg aatcactgca
    14041 accccgtgaa gccgatctcc atccactggc cggcggcgag cgggtgctgg aagtgcgggg
    14101 tgaatatctg cccatcgtcg aactgtggaa agtgttcaac gtcgcgggcg cgaaaaccga
    14161 agccacccag ggaattgtgg tgatcttaca aagtggcggt cgccgctacg ccttgctggt
    14221 ggatcaatta attggtcaac accaggttgt ggttaaaaac cttgaaagta actatcgcaa
    14281 agtccccggc atttctgctg cgaccattct tggcgacggc agcgtggcac tgattgttga
    14341 tgtctccgcc ttgcaggcga taaaccgcga acaacgtatg gcgaacaccg ccgcctgaat
    14401 gagtaaaaag gtaacaatat gaccggtatg acgaatgtaa caaagctggc cagcgagccg
    14461 tcaggccagg aatttctggt atttaccctt ggtgatgaag agtacggtat tgatatcctg
    14521 aaagtgcagg agatccgtgg ctacgatcag gtaacacgga ttgcgaacac gccagcgttt
    14581 atcaaaggcg tcacgaatct gcgcggcgtt attgtgccga ttgttgactt acgaattaag
    14641 ttcagccagg tggatgtgga ctataacgac aacacggtag ttatcgtcct gaatctcgga
    14701 cagcgggtgg tcggcatcgt ggttgacggc gtctcagacg tgctttcatt gacggcggag
    14761 caaattcgtc cggcaccgga atttgccgtg acgctttcaa cagaatatct cactggactg
    14821 ggcgcactgg gcgaccggat gttgattctg gtgaacatcg aaaaactgct gaacagcgaa
    14881 gagatggcgc tgttagatag cgcggcgtca gaagtggcgt aattctcccg atttcctcaa
    14941 ttgaaatgaa cccgatgatc tgcgcatcgg gttttttatt tcaatttcgc ggcgggtggc
    15001 atcagcaata aagtttcccc cctccttgcc gataacgaga tcaacttgtt ttcaggaagg
    15061 tgccttatga ttaaccgtat ccgcgtagtc acgctgttgg taatggtgct gggggtattc
    15121 gcactgttac agcttatttc cggcagtctg tttttttctt cccttcacca tagccagaag
    15181 agctttgtgg tttccaatca attacggaaa cagcagggcg agctgacgtc aacctgggat
    15241 ttaatgctgc aaacgcgcat taacctgagt cgttcagcgg tacggatgat gatggattcc
    15301 tccaatcaac aaagtaacgc caaagttgaa ttgctcgata gcgccaggaa aacattggcg
    15361 caggcagcga cgcattataa aaaattcaaa agcatggcac cgttacctga aatggtcgct
    15421 accagtcgta atattgatga aaaatataaa aactattaca cagcgttaac tgaactgatt
    15481 gattatctag attatggcaa tactggagct tatttcgctc agccaaccca gggaatgcaa
    15541 aatgcaatgg gcgaagcgtt tgctcagtac gccctcagca gtgaaaaact gtatcgcgat
    15601 atcgtcactg acaacgcaga tgattaccga tttgcccagt ggcaactggc ggttatcgcg
    15661 ctggtggtgg tattgattct gctggtggcg tggtacggca ttcgccgtat gttgcttaca
    15721 ccgctggcaa aaattattgc gcatattcgc gaaatcgccg gtggtaacct ggcgaatacc
    15781 ctgaccattg acgggcgcag tgaaatgggc gacctggcgc agagcgtttc acatatgcaa
    15841 cgctctttga ctgacaccgt cactcatgtg cgcgaaggtt cagatgccat ctatgccggt
    15901 acccgtgaaa ttgcggcggg caacaccgat ctttcctccc gtactgaaca gcaggcatcc
    15961 gcgctggaag aaactgccgc cagcatggag cagctcaccg cgacagtgaa gcaaaacgcc
    16021 gataacgccc gccaggcctc gcaactggcg caaagtgcct ccgacaccgc ccagcacggc
    16081 ggcaaagtgg tggatggcgt agtgaaaacg atgcatgaga tcgccgatag ttcgaagaaa
    16141 attgccgaca ttatcagcgt tatcgacggt attgccttcc agactaatat cctcgcgctg
    16201 aatgccgcgg ttgaagccgc gcgtgcgggt gaacagggcc gtggttttgc cgtggtggcg
    16261 ggtgaagtgc gtaatcttgc cagtcgcagc gcccaggcgg caaaagagat caaagccctc
    16321 attgaagact ccgtcgcacg cgttgatacc ggttcggtgc tggtcgaaag cgccggggaa
    16381 acaatgaaca atatcgtcaa tgctgtcact cgcgtgactg acattatggg cgagattgca
    16441 tcggcatcgg atgaacagag ccgtggcatc gatcaagtcg cattggcggt ttcggaaatg
    16501 gatcgcgtca cgcaacagaa cgcatcgctg gtgcaggaat cagctgccgc cgcagctgcg
    16561 ctggaagaac aggcgagtcg tttaacgcaa gcggtttccg cgtttcgtct ggcagccagc
    16621 ccactcacca ataaaccgca aacaccatcc cgtcctgcca gtgagcaacc accggcacag
    16681 ccacgactgc gaattgctga acaagatcca aactgggaaa cattttgacg agtatttact
    16741 aacgcggtca ttgccgcctg atggggagcg ttgatgttta atcgtattcg aatttcgacc
    16801 acgctgtttt taattttgat tctctgcggg atcttgcaga ttggcagtaa cggcatgtct
    16861 ttttgggcat ttcgcgacga tttgcaacga ctgaatcagg tcgagcagag caatcagcaa
    16921 cgtgcggcat tagcgcaaac tcgggcggta atgttacagg ccagtaccgc gctgaacaaa
    16981 gcgggcactc tgacggcact tagctatccg gcggatgaca ttaaaacgtt gatgacgacg
    17041 gcgcgcgcca gtctgacgca atccaccacg ctgtttaaaa gttttatggc gatgactgcg
    17101 ggcaacgagc acgtcagggc attgcaaaaa gagacggaga aaagttttgc ccgctggcac
    17161 aacgatctcg aacatcaggc gacctggctt gaaagtaatc aactttcgga tttcctcact
    17221 gcgccggtgc agggatcaca gaatgcgttt gacgttaact ttgaggcctg gcagctggag
    17281 atcaaccatg tgctggaagc cgccagtgcg caaagccagc gtaactatca gatttcggca
    17341 ctggtgttta tcagcatgat tattgttgcg gcgatctaca tcagcagtgc gctgtggtgg
    17401 acgcgcaaga tgattgttca accactggcc attatcggta gccattttga cagcattgct
    17461 gcgggtaatc tggcgcgtcc gattgcggta tatggtcgta atgagatcac cgccattttt
    17521 gccagtctga agaccatgca gcaggctttg cgtgggacgg taagtgatgt gcgtaaggga
    17581 agccaggaga tgcacattgg tatcgcggag attgtcgcag gcaataacga tctctcaagt
    17641 cgtaccgaac agcaggcggc atcgctggca caaacggccg ccagtatgga gcaattaacc
    17701 gccacggtag ggcaaaacgc cgataacgca cgacaggcgt cggaactggc aaaaaatgcc
    17761 gcgacaacgg cgcaggcggg cggtgttcag gtcagtacca tgactcacac catgcaggag
    17821 atcgccacca gctcgcaaaa aattggcgac attatcagcg ttatcgacgg aattgctttc
    17881 cagaccaata ttctggccct gaatgcggca gtggaagcgg ctcgcgccgg agagcagggg
    17941 cgtggttttg cggtagtggc aggtgaagtg cgcaatcttg ccagccgtag cgcgcaggca
    18001 gcaaaagaga tcaaagggct gatcgaagag tcagtcaatc gtgtccagca gggttcgaaa
    18061 ctggtgaata acgccgccgc gaccatgatc gatattgtca gttcggtgac ccgcgtgaac
    18121 gacattatgg gagaaattgc ctctgcgtcg gaagaacaac agcgggggat tgagcaggtt
    18181 gcacaggctg tcagccagat ggatcaggtg actcagcaga acgcctcgct ggtagaagaa
    18241 gcggcggtgg caacggaaca actggcgaat caggccgacc atctttcgtc gcgtgtggcg
    18301 gtatttaccc ttgaagaaca tgaagtagca cgacatgagt cggtgcagtt acaaattgcg
    18361 ccagtggtat cctgaagtga ttgagaaggc gctatgactt catctctgcc ctgtgggcaa
    18421 acgtctttat tgttacagat gaccgagcgc ctggcgcttt ccgacgcgca ttttcggcgg
    18481 ataagtcaat tgatctatca acgagccggg atcgttctgg ctgaccataa acgcgacatg
    18541 gtttacaacc gactggttcg tcgtttgcgt tcgctgggac tgacggattt cggtcattat
    18601 ctgaacttgc tggaatctaa tcagcacagc ggtgagtggc aggcgtttat caattcgctg
    18661 accacgaatc tgacggcatt tttccgtgag gcacaccatt tccctctgct cgcggatcac
    18721 gcacgtcgcc gttctggcga gtatcgcgta tggagcgcgg cggcttcgac cggcgaagag
    18781 ccgtacagca ttgcgatgac gctggctgac acattgggca ccgcgcccgg acgctggaaa
    18841 gtgtttgcca gtgatatcga caccgaagtg ctggaaaaag ccagaagcgg tatttatcgc
    18901 catgaagagt tgaaaaacct gacgccgcag caactgcaac ggtatttcat gcgagggacg
    18961 ggaccgcatg aagggctggt acgcgtgcgt caggagctgg cgaactatgt tgattttgcc
    19021 ccgctgaatc tactggcgaa acagtacacc gtgccggggc cgtttgatgc gatcttctgt
    19081 cgtaacgtca tgatctactt cgatcaaaat acccagcagg agattttgcg ccgctttgtt
    19141 ccgctcctta aacccgacgg attgctgttt gcgggtcact ctgaaaactt tagccacctt
    19201 gagcgccgct tcacgctgcg tggtcagacg gtgtatgcgc taagtaagga ttaacgatga
    19261 gcaaaatcag ggtgttatct gtcgatgatt cggcactgat gcgccagatc atgacagaaa
    19321 tcatcaacag ccatagcgac atggaaatgg tggcgaccgc gcctgatccg ctggtcgcgc
    19381 gtgacttgat taagaaattc aatcccgatg tgctgacgct ggatgttgaa atgccgcgga
    19441 tggacggact ggatttcctc gaaaaattaa tgcgtttgcg tccaatgccc gtagtgatgg
    19501 tttcttccct gaccggcaaa gggtcagaag tcacgctgcg cgcgctggag ctgggggcga
    19561 tagattttgt caccaaaccg caactgggta ttcgcgaagg aatgctggcg tatagcgaaa
    19621 tgattgctga aaaggtgcgt acagcagcaa aggcgagcct tgcagcacat aagccattgt
    19681 cggcaccgac aacgctgaag gcagggccgt tgttgagttc tgaaaaactg attgcgattg
    19741 gtgcttcaac gggtggaact gaggcaattc gtcacgtact gcaaccgttg ccgctttcca
    19801 gcccggcact gttaattacc cagcatatgc cgcctggttt cacccgctct tttgccgaca
    19861 gacttaataa gctttgtcag atcggggtta aagaagccga agacggagaa cgtgttttgc
    19921 cggggcatgc ctatatcgcg ccgggcgatc ggcatatgga gctggcgcgt agtggcgcaa
    19981 attaccaaat caaaattcat gatggcccgg cggttaaccg tcatcggcct tcggtagatg
    20041 tgttgttcca ttctgtcgcc aaacaggcgg ggcgtaatgc ggttggggtg atcctgaccg
    20101 gtatgggcaa cgacggcgcg gcaggaatgt tggcgatgcg tcaggcgggg gcatggaccc
    20161 ttgcgcaaaa cgaagcaagt tgcgtggtgt tcggcatgcc gcgcgaggcc atcaatatgg
    20221 gtggtgtctg cgaagtggtc gatcttagcc aggtaagcca gcaaatgttg gcaaaaatta
    20281 gtgccggaca ggcgatacgt atttaaatca ggagtgtgaa atggcggata aagaacttaa
    20341 atttttggtt gtggatgact tttccaccat gcgacgcata gtgcgtaacc tgctgaaaga
    20401 gctgggattc aataatgttg aggaagcgga agatggcgtc gacgctctca ataagttgca
    20461 ggcaggcggt tatggatttg ttatctccga ctggaacatg cccaatatgg atggcctgga
    20521 attgctgaaa acaattcgtg cggatggcgc gatgtcggca ttgccagtgt taatggtgac
    20581 tgcagaagcg aagaaagaga acatcattgc tgcggcgcaa gcgggggcca gtggctatgt
    20641 ggtgaagcca tttaccgccg cgacgctgga ggaaaaactc aacaaaatct ttgagaaact
    20701 gggcatgtga ggatgcgact atgatgcaac catcaatcaa acctgctgac gagcattcag
    20761 ctggcgatat cattgcgcgc atcggcagcc tgacgcgtat gctgcgcgac agtttgcggg
    20821 aactggggct ggatcaggcc attgccgaag cggcggaagc catccccgat gcgcgcgatc
    20881 gtttgtacta tgttgtgcag atgaccgccc aggctgcgga gcgggcgctg aacagtgttg
    20941 aggcgtcaca accgcatcag gatcaaatgg agaaatcagc aaaagcgtta acccaacgtt
    21001 gggatgactg gtttgccgat ccgattgacc ttgccgacgc ccgtgaactg gtaacagata
    21061 cacgacaatt tctggcagat gtacccgcgc ataccagctt tactaacgcg caactgctgg
    21121 aaatcatgat ggcgcaggat tttcaggatc tcaccgggca ggtcattaag cggatgatgg
    21181 atgtcattca ggagatcgaa cgccagttgc tgatggtgct gttggaaaac atcccggaac
    21241 aggagtcgcg tccaaaacgt gaaaaccaga gtttgcttaa tggacctcag gtcgatacca
    21301 gcaaagccgg tgtggtagcc agtcaggatc aggtggacga tttgttggat agtcttggat
    21361 tttgatttgt attgcctgat gtggcgtgac cacgtcatat caggcggtct gataaggcga
    21421 tgacgccgca tccgacaaac gcacactatg cttgatgtgt ggcagcaaaa gccctaaatc
    21481 ccgcctgttt tgccccttac tcaaaccatt gaacgctttg cgctctggca tcattcacgc
    21541 ttaatactct ttccaggatt ggcgacgtgt ctgacgagag cgacgacaaa acagaagccc
    21601 ccacacctca ccgactagaa aaagcgcggg aagaggggca aatcccgcgt tcccgtgaac
    21661 tgacctcact gctgattttg ctggtgggcg ttagtgttat ctggtttggc ggtgtgtcgc
    21721 tggcccgtcg attgtcgggc atgctctccg ctgggctgca ttttgatcac agtattatca
    21781 aagacccgaa tctgatcctc gggcagatta ttctgctgat cagagaagcc atgctggcgc
    21841 tgctgccact gattagcggc gtggtactgg tggcgctcat ttctccggtc atgctgggag
    21901 ggctggtatt tagcggcaaa tccttgcagc cgaagttttc caaactcaac ccgctaccgg
    21961 gcattaaacg gatgttctcg gctcagactg gcgcggagtt gcttaaagca attttgaaaa
    22021 ccatcctggt tggcagcgtg acggggtttt ttctctggca tcactggccg cagatgatgc
    22081 gcttaatggc cgagtctccg attaccgcca tgggtaatgc gatggatttg gtagggctat
    22141 gcgcactgct ggtggtgctt ggtgtcattc caatggtggg atttgacgtc tttttccaaa
    22201 tcttcagcca cctgaaaaag ctgcgaatgt cgcggcagga tattcgtgat gagttcaaac
    22261 aaagcgaagg cgacccccat gttaaaggac ggatccgtca gatgcagcga gctgccgcac
    22321 ggcgtcggat gatggccgat gtgccgaaag cggatgtcat tgtcaataac ccgacccact
    22381 attcggtagc gttgcagtat gacgaaaaca aaatgagcgc accgaaagtg gtcgctaaag
    22441 gtgcagggct ggtcgcgctg cgcattcgtg aaattggcgc tgaaaataac gtcccgacgc
    22501 ttgaagcgcc gccgctggcg cgagcgctgt atcgacatgc ggagattggt caacaaatcc
    22561 cgggtcaact gtacgccgcg gtggcggaag tgctggcctg ggtctggcaa ctgaaacgct
    22621 ggcgtctggc tggtggacag cgccctgtac aacctactca tcttccggtg ccggaagccc
    22681 tggattttat taacgagaaa ccgacccatg agtaatctgg ccgcgatgct gcgcctgccc
    22741 gcaaacctga aatcgacaca atggcagatc cttgccggac cgattttgat cctgttgatc
    22801 ttgtcgatga tggtgctgcc actgcccgca ttcatactcg acctgttgtt taccttcaat
    22861 attgccttgt cgatcatggt gttgctggtg gcgatgttta cccagcgcac gcttgagttt
    22921 gctgcgtttc cgaccattct gttgtttacc acgctgttgc gtctggcact taacgtggct
    22981 tcaacccgta tcattttaat ggaagggcat accggcgcgg cggcggcagg gaaggtggtc
    23041 gaagcgttcg gtcacttcct cgttggtggc aatttcgcta tcggtatcgt ggtgtttgtc
    23101 attctcgtga tcatcaactt tatggtcatt accaaaggtg ccgggcgtat cgcagaagtg
    23161 ggtgcgcgct ttgttctcga tggtatgccg ggtaagcaga tggcgattga cgccgacctt
    23221 aacgccggat tgattggtga agatgaggcg aaaaaacgcc gctccgaagt gactcaggaa
    23281 gccgattttt acggctcaat ggacggggca agtaagtttg ttcgcggcga tgccatcgcc
    23341 gggatcctca tcatggtcat taacgttgtc ggcgggttgc tggtcggcgt gctgcaacat
    23401 ggcatgagca tgggacacgc ggcggaaagt tatacgctat tgaccattgg cgacggtctg
    23461 gtggcacaaa ttccggcgct ggtgatttct accgccgcgg gggtcatcgt tacgcgtgtc
    23521 agcaccgatc aggatgttgg cgagcagatg gtgaatcagc ttttcagtaa cccaagcgtt
    23581 atgttgttaa gcgccgccgt gctcggttta ctcggcctgg tgcctggaat gccgaacctg
    23641 gtatttttgc tgttcactgc cggattgctc gggctggcct ggtggatacg cggacgcgaa
    23701 caaaaagcgc ctgccgaacc caaaccggta aaaatggcag agaataatac cgttgtcgaa
    23761 gcgacgtgga acgatgtaca actggaagat tctctgggaa tggaagtggg ttatcgactg
    23821 atcccgatgg tcgatttcca gcaggatggt gagttgttgg gccgtatacg cagtatccgc
    23881 aagaaatttg cccaggagat gggatttctg ccgccagtgg tgcacattcg cgacaatatg
    23941 gatctgcaac ctgcccgcta tcgcattttg atgaaaggcg tggagattgg cagtggtgat
    24001 gcttatccgg ggcgctggct ggcgattaac cctggaaccg ctgccgggac gttacctggt
    24061 gaggcgaccg tcgatccggc atttggcctg aatgctatct ggattgaaag tgcgctaaaa
    24121 gaacaggcgc agattcaggg gtacacagtg gttgaggcca gcacggtggt agcaacgcat
    24181 cttaaccacc tcattagcca gcatgccgca gagctgtttg gtcgtcagga ggcgcaacag
    24241 ctgttggatc gcgtcgccca ggagatgcca aagctgacgg aagatctcgt tcctggcgtc
    24301 gtcacgctca ccacactgca taaagtgctg caaaatctcc tcgatgaaaa agtaccgatt
    24361 cgcgatatgc gcaccattct cgaaacgctg gcggaacatg cgcccatcca aagcgatcca
    24421 catgaattaa ccgccgtcgt gcgcgtggcg ttgggacggg cgattaccca gcagtggttt
    24481 cctggcaaag atgaagtcca tgttattggc ctcgatacac cgctggaacg tttgttacta
    24541 caggcgctgc agggcggggg aggactggag ccagggctgg cggatcgttt actggcgcaa
    24601 actcaggaag cgctatcccg tcaggagatg ctgggtgcgc cgccagtatt gttggtgaac
    24661 cacgcgctgc gaccattatt gtctcgcttc ctgcgccgca gcttgccgca gttagtggtc
    24721 ctgtcgaatc tggaactgtc tgataaccga catatccgca tgacggcgac aattggcggc
    24781 aaataatgag aaccttatta gcaatattat tgtttccgct gctggtgcaa gccgccgggg
    24841 aggggatgtg gcaggcaagt agtgtaggaa ttacgctaaa tcatcgcggt gagtcgatgt
    24901 cgtctgcgcc tctttctacg cgacaacctg cttcaggatt gatgacgctg gtagcgtggc
    24961 gttatcagct tatcggcccg acaccttcag gactgcgggt tcgcttgtgt tcgcaatctc
    25021 gttgtgtcga attagagggg cagagcggaa ccaccgtggc cttttccggt atagcggcag
    25081 cagaaccgtt gcgatttatc tgggaagtgc ctggcggtgg gcggttaatt ccgtcgctga
    25141 aagtacaacg taatgaagtg attgtgaatt atcgctgaag gacaaaaaaa attacgcgct
    25201 cgcgagtatg tttaataact cagtgaaaaa ggctggatgt tttgttgcaa tcatttatca
    25261 ggaatagatt gcaacaaaac atgccaaaaa tatgtgaatt atcctcttgt caaagggctt
    25321 ggataaaaat cttcagagtt acggtatagc tgcaactggc gagttcgcgt attggtctgt
    25381 atgatcatac atgagagcca ttctgattca gaataagggt cacttaaatc ctgaacgtcc
    25441 tcatttgaaa ggattaattc acattctttg tccctaaaat ccagccaggc tcgctgtgat
    25501 tttttaagca agttaagttg ttcggtatca ggttttttta cacctgtgcg tggcacttgt
    25561 tctatataac gataagcttc accataggct ttatttaatt gcgcatcgac gagattgtat
    25621 tcttgtcgca ggcatgattt gatcacgtta acgtcagtat catcatctga atttttttgc
    25681 caacaatctt tgatggctga aatgtcatgg tcgtttacta taaccgtatc ttctgcgtaa
    25741 gcctggcaag agataatgcc taatgttaat acaagaaatt taaacactgc ggtattcctt
    25801 aaattcaacc gttatcgttt ccctggggcc ggatattacg cttctggcct cttatccaac
    25861 agtgagaaaa caatggcatg tccattttca gaatacattt agatggcaat aaaaaagcct
    25921 gatcaggtaa ccctgatcag gctttactct cacgaaaaat cgattacata cgctctactg
    25981 tctcaatacc cagcgtatcc agacccagct ttagcgtctt cgctgtcagt tgtgccagtt
    26041 tcagacggct gttacgcact tcttcgtttt ctgcgctgag gatcgggcag tgctcgtaga
    26101 agccagagaa cagaccagcc agatcgtaca ggtaagcaca catcacatgc ggcgtgcctt
    26161 cacgggcaac cacggtgagg gtttcttcaa actgcagcag gcgagctgcc agttgcgctt
    26221 cacgatcttc acggatgatt accggagctg cagccagttg ctcttcgtta atttctgcct
    26281 tacggaacac ggacaataca cgcgtgtaag catactgcat gtatggcgcg gtattaccct
    26341 caaacgccag catgttgtcc cagtcgaaga tgtagtccgt ggtgcggttt ttggagagat
    26401 ccgcatattt caccgcacca ataccaaccg cgttagccag tttttccagc tcgtcagctg
    26461 gcatatccgg gttcttttct gccaccagac ggcgtgcacg ttccagggct tcatccagca
    26521 gatcggccag tttcactgta ccacccgcgc gggttttgaa cggtttgccg tctttaccca
    26581 gcatcatgcc gaacatgtgg tgttccagcg gtacggattc cggtacatag cctgctttac
    26641 ggacgatcgc ccatgcctgc atcaggtgtt gatgctgacg ggagtcgatg taatacagca
    26701 cgcgatcggc atgcagtgtt tcataacgat atttcgcaca ggcaatatca gtggtggtgt
    26761 agagatagcc gccatctttc ttctgaatga tcacgcccat cggttcgcct tccttgtttt
    26821 tgaactcatc aaggaatacg acggtcgcgc cttcactttc tactgccaga cctttggctt
    26881 tgagatccgc cacaattcct ggcagcatcg ggttgtagag gctttcgccc atcacgtcat
    26941 cacgggtcag cgtcacgttg agacgatcgt aggtgatctg gttctgcgtc atggtgatgt
    27001 cgaccagttt gcgccacatc tcgcggaaat attcgtcacc gctttgcagt tttaccacgt
    27061 agttacgtgc acgctcggca aactcttcat cttcgtcgta atgctttttc gcatcgcggt
    27121 agaaaccttc aaggtcagcc agctccattt caccggcgtt ttcctgctgc tgcttttcca
    27181 gccatgcaat cagcataccg aactgagtgc cccagtcgcc gacgtggttt gcgcgaatca
    27241 ctttgtgacc gaggaactcc agagtacgca ctgctgcgtc accaataatg gtagagcgca
    27301 ggtgaccgac gtgcatctct ttcgccacgt ttggcgcaga gtagtcaacc acaatggtct
    27361 gtttttctgg cgtagcaaca ccgagacgat cggacgccag cgcctgctga acatgttcag
    27421 ccaggaatgc cggatcaagg aaaatgttga taaagcctgg accggcgatc tcaactttgc
    27481 tggcgatacc gttaagatcc agatgagtca gcacctgctc tgctaattgt cgcggtgcca
    27541 tacccagttt tttagcaact gccatcatgc cgttagcctg atagtcgccg aactgaactt
    27601 ttgctgactg acgaacctgc ggttcgcaat ccgcaggcgc gcctgccgca atcatggcct
    27661 gacggacttt ttctgagaga agagcctgaa tattcaccgg aataccttac attgatgaag
    27721 cgagcaaaaa ttgcccgcac cagttaaaga aattagggcg ggagtatact gcaaatggct
    27781 gttttcgtca gcaacgtgcg cgtggcgtat tgccgttttc actattgcat cagaagcggc
    27841 gtgaaatcag cggttggttg gcacagggca acagggtaaa ttaacgcttt tactgaaaga
    27901 cgaagagtga ttatggcgaa ctggcaatct atcgacgagc tgcaggatat tgcatcggat
    27961 ttaccgcgat ttacccacgc attagatgaa ctttcccgcc gtctggggtt gaatatcact
    28021 ccgctgactg ccgatcacat ttctttgcgt tgccatcaaa acgccactgc tgaacgctgg
    28081 cgtcgcgggt ttgaacagtg tggcgagctt ttgtcagaaa atatgatcaa tggcagaccg
    28141 atttgtctgt ttaaattgca tgaaccggta caggttgcgc actggcagtt ttctattgtg
    28201 gagctaccgt ggcccgggga aaaacgttac ccacacgaag gttgggaaca tattgaaatc
    28261 gttctgccgg gtgatccaga aacgctgaat gcccgtgcgc tggcattgct ttctgatgaa
    28321 ggactcagcc taccggggat ctcagtgaaa accagttcgc caaaaggtga gcatgagcga
    28381 ttaccgaatc cgacgctggc ggtcaccgat ggcaaaacaa ctattaagtt tcatccgtgg
    28441 tccattgaag agatcgttgc cagcgagcag tcggcgtaat taaggagtaa aaatggcatt
    28501 actggaaatt tgctgttaca gcatggaatg tgcactaacg gcgcagcaaa acggcgcaga
    28561 cagagttgaa ttatgcgcag ccccaaaaga ggggggctta acgccgtcgt tgggtgtact
    28621 gaaatccgtg cgccagcggg tgacgatccc tgtgcatccg ataattcgcc cacgcggtgg
    28681 tgatttttgc tacagcgacg gtgagtttgc cgccattctt gaggatgtgc gcacggtccg
    28741 cgaattaggt tttcctggac tggtgacggg cgttctcgat gttgacggga atgtcgatat
    28801 gccacgaatg gaaaaaataa tggctgctgc cggtccgctg gcagtgactt ttcatcgcgc
    28861 ctttgatatg tgcgctaacc ctctaaatac actcaataat cttgcggaat tgggcattgc
    28921 ccgggtgctg acatcagggc aaaaatcaga cgcactgcaa ggtttatcaa aaattatgga
    28981 acttattgcc catcgtgatg ctccaatcat tatggccgga gcaggagtcc gtgcagaaaa
    29041 cctgcaccac ttcctcgatg ccggagtgct ggaagtccat agctccgcgg gagcgtggca
    29101 agcctcaccg atgcgttatc gtaatcaagg attgtctatg tcatcagatg cacacgcgga
    29161 cgagtattcg cgttatgtcg tagacggggc ggcggttgct gaaatgaaag gaatcattga
    29221 acgccatcag gccaaatgat ttttaccgtt gcatcatgtc gcccaatatg atgcttgctc
    29281 gtaccaggcc cctgcaattt caacaggggc ctttttttat ccctgagcag tataaaaaac
    29341 gaacgataac cgtgatctgt tgagcgggtg acagtgcgca tagcgttgtg ctaaaaatat
    29401 tttttatatt cacattgatt atgggattta aattgctaaa actgataaat atatattcta
    29461 aatagcaact gggttattcc ttagcaatta atgattacat tgtaataaat catattcttt
    29521 atcgattatt tcaggcagtg ttgtgccgag ttatgcaagc ggttaattcg ttgtgtattt
    29581 aattatacaa tgatttcggt gttctggaat ttatttagag gaatctatgc gagggaaaaa
    29641 acgcattggg ttattgtttt tgctgatagc ggttgtggtt ggtggcggcg ggttattgct
    29701 ggcgcaaaaa gccttacata aaacgtcgga tacagcattt tgcctttcct gccactcgat
    29761 gagtaaacct tttgaggaat atcagggaac tgtccacttt tcgaaccaga aagggatacg
    29821 tgcggaatgt gccgattgcc atattccaaa gtcagggatg gattatttat ttgctaaatt
    29881 aaaagcatct aaagatattt atcatgaatt tgttagcggc aaaatagaca gtgacgataa
    29941 gttcgaaact catcgccagg aaatggccga aacagtatgg aaagaattaa aagcaactga
    30001 ctctgcaacg tgccgtagtt gccattcttt tgatgccatg gatattgcct cgcaaagtga
    30061 atctgcgcag aaaatgcata acaaagcaca aaagggcggc gaaacctgta tcgattgtca
    30121 taaaggcatt gcccattttc cgccagaaat aaaaatggat gacaacgcgg cgcatgagct
    30181 ggaaagtcag accgctactt cagtgactaa tggcgcacat atttatcctt tcaaaacttc
    30241 tcgcataggc gagctggcta ccgtgaatcc tggtaccgat ctcgccgtcg ttgatgccag
    30301 tggcaaacag ccgatcgttc tgttgcaggg ttatcaaatg cagggcagtg aaaacacgct
    30361 ctacctggcg gcaggtcaac ggctggcgct agccacatta agtgaagaag gtatcaaggc
    30421 gctcacggta aacggggaat ggcaggctga cgaatacggc aatcaatggc gtcaggcgtc
    30481 tttacagggt gcgcttaccg atcccgcatt agcggaccgt aaaccgctat ggcaatacgc
    30541 tgaaaaactt gacgatacct attgcgctgg ttgtcatgcc cctattgccg ccgaccatta
    30601 caccgtcaat gcgtggccgt ccattgccaa aggaatgggg gcacgaacca gcatgagcga
    30661 aaacgaactg gacattttaa cgcggtattt ccagtacaac gccaaagata ttaccgagaa
    30721 acagtgatcc tcgttgatca gggaggaagt tatgacatta acaagacgtg aatttattaa
    30781 acacagcggt attgccgctg gagcgctggt ggtgacatcg gcagcaccgc tacccgcatg
    30841 ggcagaagag aagggcggta aaatcctcac cgcgggacgc tggggagcga tgaacgtaga
    30901 agtgaaggac ggcaagattg tttcttcgac aggcgcgctg gcgaaaacca taccgaactc
    30961 cttacagtct acggcggcgg atcaggtaca caccacggcg cgtattcagc atccgatggt
    31021 gagaaaaagc tatctcgata atccactgca accggcgaaa ggtcgaggcg aagataccta
    31081 tgtacaggtg agctgggaac aagcattaaa actgattcat gagcaacacg atcgtattcg
    31141 taaagctaac ggaccatcgg ccatttttgc cggatcttac ggctggcgct cttctggcgt
    31201 gttgcataaa gcgcaaacct tactgcaacg ttatatgaac ctcgcgggcg gttattccgg
    31261 gcatagcggc gattattcaa ccggggcagc acaggtgatc atgccgcatg tggtcggttc
    31321 tgtagaggtg tatgaacagc agacctcctg gccgctgatt ctggaaaaca gccaggttgt
    31381 ggtgctgtgg gggatgaacc cgctgaatac cttaaaaatt gcctggagca gtaccgatga
    31441 gcaagggctg gaatacttcc atcagctgaa aaaatctggc aaaccagtga ttgccattga
    31501 tcctatccgc tccgaaacca ttgagttctt tggcgataat gccacctgga tcgcgccgaa
    31561 tatgggcacc gacgtggcac tgatgttagg gattgcgcat accctgatga cacaaggcaa
    31621 acacgataaa gtgttccttg aaaaatacac caccgggtat ccgcagtttg aagaatatct
    31681 gacaggtaag agcgataata ctccgaagag cgcggcctgg gcggcggaaa ttactggcgt
    31741 accggaagcg cagatagtca aactcgctga actgatggcg gcgaatagca caatgctgat
    31801 ggcaggctgg ggaattcagc gccagcaata tggtgaacag aaacactgga tgctggtgac
    31861 gctggcggcg atgttagggc agattggtac accgggcggc ggttttggtt tctcttatca
    31921 ttactccaat ggtggcaacc cgacacgtgt tggcggcgtg ctgccggaaa tgtctgcggc
    31981 gattgccgga caggccagcg aggccgctga tgatggcgga atgacggcga ttcctgttgc
    32041 ccgtatcgtc gatgcgctgg aaaatcctgg cggcaaatat cagcataacg gtaaagagca
    32101 aacgtatccg aatatcaaaa tgatctggtg ggcgggtggc ggtaacttta cccatcacca
    32161 ggatactaat cgtctgatta aagcatggca gaaaccggag atgatcgtcg tttctgaatg
    32221 ctactggacc gcagctgcta aacatgcaga tatcgtatta ccgatcacca catcgtttga
    32281 gcgcaatgac ttaacgatga ccggtgatta cagcaaccag catattgtgc cgatgaagca
    32341 ggttgttgct ccgcaatttg aagcgcgaaa cgattttgat gtgtttgccg atcttgcgga
    32401 attactcaaa cctggcggaa aagagatcta taccgaaggt aaagatgaaa tggcgtggct
    32461 gaaatttttc tatgatgccg ctcagaaagg tgcccgtgcg caacgcgtga ctatgccgat
    32521 gtttaatgcc ttctggcagc aaaataaact gatcgaaatg cgccgcagcg agaagaacga
    32581 acagtacgtt cgttatggtg atttccgcgc cgatccggtg aaaaatgcgc tgggtacgcc
    32641 aagcggcaaa attgagattt actccaaaac gctggaaaaa tttggctata aggattgccc
    32701 ggcacaccca acctggcttg cgcctgatga gtggaagggt accgccgacg agaagcagtt
    32761 gcagcttctg accgcacatc cggcacaccg tttacatagt cagcttaact atgcggaact
    32821 gcgtaaaaaa tatgcggttg cagatcgtga accaatcact attcacaccg aagatgctgc
    32881 tcgctttggt attgcgaatg gtgatctggt gcgtgtgtgg aacaaacgtg gtcagattct
    32941 gacaggcgcg gtggtgactg acgggatcaa aaaaggcgtg gtatgcgtgc atgaaggtgc
    33001 atggccagat ctggaaaatg gcttgtgtaa aaacggcagt gcgaacgtgt taacggcgga
    33061 tatccccagc tcgcagctgg caaatgcctg tgccggtaac tctgcgctgg tgtatatcga
    33121 aaaatatacg ggcaatgcgc cgaagttaac ggcgtttgat cagccagcta ttcaggcata
    33181 agaagtgaat agcgcccgga aacgggcgct gaggttatgc aaacgtaaag ctgcctgatg
    33241 cgctatgctt atcaggccta catagtccct gcaatatatt gaattagcaa ggttttgtag
    33301 gccggataag gcgttcacgc cgcatccggc agtaatacca gacctttacg gcttgcgcgc
    33361 aatcagcacc gcacgtttag gtgccggata accttcaact gtcttactcg gatcatgtgg
    33421 gtcgaggaaa tcggccagcg attcagtaac catccattcg gtgcgtcgtt gctcttctgt
    33481 agtggtaacg ctcacatctg caatgcggat atctacaaaa ccacacttct tcagccagtt
    33541 tttcagcgcc agcgcggaag ggatgaaata gacattacgc atttgcgcgt aacgatcgcc
    33601 tggtaccagc accgtgttct catcaccatc aataaccagc gtttccagca ccagttcacc
    33661 ttcattgacc agttgatctt tcaactgcca tagatgctcc agcggtgaac ggcgatggta
    33721 aagcacgccc atcgaaaaga cggtatcaaa ggctttcagt gccggaagtt gttcaatacc
    33781 taacggtaac aaatgcgcgc gctgatcatt acccagtagt ttacgcaccg cttcaaactg
    33841 gcacagaaac agttgcgtag ggtcgatccc caccgcgagg tgcgcgcctg cgccaatcat
    33901 gcgccacatg tgataaccgc tgccgcagcc gacatccaga atggtgcgtc cggttaaatc
    33961 agaaagatgg ggcagaacac gatcccattt ccagtcggaa cgccattcgg tatcgatgtt
    34021 gacgccatac agtgagaacg gccctttgcg ccacggcatc aggttgcgca tcagcgtttc
    34081 aatgcgttta atttgccctg cgctcagtgg cccttcgctt tctgccgtaa cgctatgcaa
    34141 taaatcaaga cgatacggtt taatttcagg tagaaactcc accgcatttg accactgctt
    34201 aaacaacccg tgctgctgct cgcgctgcca gttagcaatc tgcgcgggca gcgtttcgag
    34261 ccagtgtgaa agatgatttt tggcaatcag agaataaaag ttaccaaagt cgatcatgca
    34321 gcgtcctctg cttttaatgc caccagtgaa ccaaagttaa agcactggaa ccacagctcg
    34381 ctatgctcaa aaccggcgtt atgcaggcgt gctttatgtg tttccacgga atcggtcagc
    34441 atcacgtttt ccagcatgct gcgcttctgg ctgatctcca gttcgctgta accgttggca
    34501 cgtttaaagt cgtggtgcat gttgaacaaa agttcaccaa ctttggcatc ttcgaaactg
    34561 aatttttccg aaagcaccag cgcgccgccc gggttcagtc cttgataaat tttatccagt
    34621 aatgcctggc gctcggaagg atccaggaat tgcagggtaa aattcagcac caccatcgat
    34681 gcgttttcaa tggcgatatc gcgaatatca ccttcaatga cgtctactgg cgtaggggct
    34741 ttataggcgt caatatgacg acggcagcgt tcaatcatcg ccggggagtt gtcgatggca
    34801 ataattttgc aattatcatg atgaatgttg cgacgcaccg agagcgtcgc cgcgcccaga
    34861 gaacaaccca gatcgtaaac ctgcgtacca ggttgaacga agcgctcggc taacatacca
    34921 atcatggaaa taatattgga atagccggga acggaacgct ggatcatatc cgggaagact
    34981 tcagctaccc gttcatcaaa ggtccagtcg cccagtctgg cgataggggc agaaaatagc
    35041 gtgtcgcggt gagacataac gtaaaaatcc gggaaaaaga aagtggcgta ttgtgcgcta
    35101 acgcagggag aaaaccaact cccagggcat ataccaaaga ttcgccagca ccatcagcaa
    35161 cagcgcacac caggtggcgc tcatgccgga acgtcgccag cggaacagac ggtgatgaaa
    35221 accgtaataa tgcatcagac gaccagcaag caaaacgatg ccgcaaatat gcaccatcca
    35281 ggtttctgcg ccattcattt ccataaacag catcaacaca atcgcgatgg gaatatattc
    35341 caccgcgtta ccatgaatgc gaatagcgct ttgcagttcg ctaaaaccgc cgtcgccata
    35401 ggcaacgcgg tactgcattc gcaggcgaac gacatcaaaa gagaacttca ttaataacaa
    35461 cgcacttaaa acggcataca gcgcgcttac catacaaact ccctttaaaa tggccgattg
    35521 cacctgtcta tgataggtgg caaattcaga aaagagaaga ttgctgtgga atagccggaa
    35581 ctgctccgat ctctggaagc gttttacgta agtcttccca cagggtatgt accagttccg
    35641 gggcctgggc aatatctggc gtatgtaaaa aaagataagg cgtagtggtc tgatgccact
    35701 gcgctaattt ctgtaaccag acctgaaata attcccggtt ttgcgtcata tcatcactac
    35761 cgataaaacg aatcagcgga tttgtcgccg tcagtacagc atgtaccgga actttaggtt
    35821 tttttcgttg agcgtcgcga atagcttcac tgtgtggaca tgctgcatga accgggcggc
    35881 tgtctaaaat cacccgatta acgccgcgct gatgtaaacc gcgattaagc gtttgttcct
    35941 cttccccttt ggcgaaaaac tgtggatggc ggacttccac accataatta aattcgccag
    36001 ggagagaatc gagaaaattc caaagcgcag gcagctcccg tgggccgaat gtggcaggca
    36061 gttgcagcca gtattgccca atgcgcggag ccaacggtga catgcgggtc aaaaattcag
    36121 tcactaaatc atcgcaatgc cgtaatgctg cctgatgcga aatggtcgcc ggaaacttaa
    36181 aacagaagcg gaagtcatct gtggtctgct cacgccagcg caggacaacc tcgggtttcg
    36241 gcagggcgta aagcgtggtg ttgccctcca cgcagttaaa gtggcgggca tactcttcaa
    36301 ggctggtgat ccccaaccgc acccatttag gatgcgacca ttgcggtaga ccgatgtaaa
    36361 tcataacgcg tgaaggatct cttccacact gcgcacacgg acgatgcgtg ggtagatatg
    36421 gttgatgctg ttattgtgct gctcggcgct ggcggcacta caggcgtctt cggcaatcac
    36481 cagattaaag ccaagttccc aggcattgcg ggcggtggat tcaacaccga tattggtcga
    36541 gatcccacat aacactattg tatcgatacc tcggcggcgt aattgcaact ccagatccgt
    36601 accgtaaaac gcaccccact gacgtttgat gatttcgata tcactgtcag ttgcacctaa
    36661 tgcagctgga tgttgccacc agttttcggg caacacttta gcgggggagg gggcatcaac
    36721 cggctgtttt aatgcttcgg cgtaatcggc agaccagcca acgcgcacca gaaacacggg
    36781 ctgaccgctg gcgcgaaatt tcgccgccag cttcccggcg cgattaacta cctcatcggc
    36841 agtatgtgga ccaccggcaa aaggtaagat gccttcttgt aaatcgatca ccaccaacgc
    36901 agtggtttta gcattaagtt caagcatgct gactcctgaa aaaacaaaat cgtgcctcac
    36961 accttaatgc gattagccga tggcggaacg acaaattttg ttaattttta tgagttcggg
    37021 caacaccttt atagcaaact ggcgtagagt ctgatttcag gcccgatata aagtggtaac
    37081 gaggcgttcc agcgcgggcc aggctcttac ggtgcggcag aatttccagt ataatagccg
    37141 ccttttttca ttcagttgtg acatacagct aacgctgcga cttggtcacc tgcgcagcag
    37201 ggggcatccg ccggcagcta agttaaggga tatctcatgc gtacagaata ttgtggacag
    37261 ctccgtttgt cccacgtggg gcagcaggtg actctgtgtg gttgggtcaa ccgtcgtcgt
    37321 gatcttggta gcctgatctt catcgatatg cgcgaccgcg aaggtatcgt gcaggtattt
    37381 ttcgatccgg atcgtgcgga cgcgttaaag ctggcctctg aactgcgtaa tgagttctgc
    37441 attcaggtca cgggcaccgt acgtgcgcgt gacgaaaaaa atattaaccg cgatatggcg
    37501 accggcgaaa tcgaagtgct ggcgtcctcg ctgactatca tcaaccgcac agatgttctg
    37561 ccgcttgact ctaaccacgt caacaccgaa gaagcgcgtc tgaaataccg ctacctcgac
    37621 ctgcgtcgcc cggaaatggc tcagcgcctg aaaacccgcg ctaaaatcac cagcctggtg
    37681 cgccgtttta tggatgacca cggcttcctc gacatcgaaa ctccgatgct gaccaaagcc
    37741 acgccggaag gcgcgcgtga ctacctggtg ccttctcgtg tgcacaaagg taaattctac
    37801 gcgctgccac aatccccgca gttgttcaaa cagctgctga tgatgtccgg ctttgaccgc
    37861 tactatcaga tcgttaaatg cttccgtgac gaagacctgc gcgctgatcg tcagcctgaa
    37921 tttactcaga tcgatgtgga aacttctttc atgaccgcgc cgcaagtgcg tgaagtgatg
    37981 gaagcgctgg tgcgccatct gtggctggaa gtgaagggcg tggatctggg cgatttcccg
    38041 gtaatgacct ttgcggaagc agaacgccgt tatggttctg ataaaccgga tctgcgtaac
    38101 ccgatggaac tgaccgacgt tgctgatctg ctgaaatctg ttgagtttgc agtatttgca
    38161 ggtccggcga acgatccgaa aggtcgcgtt gccgctctgc gtgttccggg tggcgcatcg
    38221 ctgacccgta agcagatcga cgaatacggt aacttcgtta aaatctacgg cgcgaaaggt
    38281 ctggcttaca tcaaagttaa cgaacgcgcg aaaggtctgg aaggtatcaa cagcccggta
    38341 gcgaagttcc ttaatgcaga aatcatcgaa gccatcctga atcgtactgc cgcgcaagat
    38401 ggcgatatga ttttcttcgg tgccgacaac aagaaaattg ttgccgacgc gatgggcgca
    38461 ctgcgcctga aagtgggtaa agaccttggt ctgaccgacg aaagcaaatg ggcaccgctg
    38521 tgggttatcg acttcccgat gtttgaagac gacggtgaag gcggcctgac ggcaatgcac
    38581 catccgttca cctcaccgaa agacatgacg gctgcagagc tgaaagctgc accggaaaat
    38641 gcggtggcga acgcttacga tatggtcatc aatggttacg aagtgggcgg tggttcagta
    38701 cgtatccata atggtgatat gcagcagacg gtgtttggta ttctgggtat caacgaagag
    38761 gaacagcgcg agaaattcgg cttcctgctc gacgccctga aatacggtac tccgccgcat
    38821 gcaggtctgg cattcggtct tgaccgtctg accatgctgc tgaccggcac cgacaatatc
    38881 cgtgacgtta tcgccttccc gaaaaccacg gcggcagcgt gtctgatgac tgaagcaccg
    38941 agctttgcta acccgactgc actggctgag ctgagcattc aggttgtgaa gaaggctgag
    39001 aataactgat atgactcaaa tacacgaaat cattcgcgtt gcatcgaggc ggcaactgag
    39061 tgaactccca tgagcataga taactatgtg aatgggatga gcgaaggcag tcaacgaaga
    39121 ggcagcgtga aggataaagt gtataagcgt cccgtttcga tcttagtggt catctacgca
    39181 caagatacga aacgggtgct gatgttgcag cggcgtgatg accccgattt ctggcagtcg
    39241 gtaaccggca gcgtggaaga gggtgaaacc gcgccgcaag ctgccatgcg cgaagtaaag
    39301 gaagaggtca ccattgatgt tgtcgctgaa caactgacct taattgactg tcagcgcacg
    39361 gttgagtttg aaattttttc acatttacgt catcgctatg cgccgggcgt gacgcgtaat
    39421 acggaatcat ggttctgtct tgcgcttccg cacgagcggc agatcgtttt cactgaacat
    39481 ctggcttaca agtggcttga tgcgcctgct gcggcggcgc tcactaagtc ctggagcaac
    39541 cggcaggcga ttgaacagtt tgtaattaac gctgcctgaa caggcagggc tatctggaga
    39601 tatttttatg gcaggtcata gtaaatgggc caacaccaga catcgtaaag ctgcgcagga
    39661 tgctaagcgc ggtaaaatct tcactaaaat cattcgtgag ctggtaaccg cggctaagct
    39721 gggcggtggc gatccggacg ctaacccgcg tctgcgtgca gcggtggata aagcactgtc
    39781 taacaacatg acccgtgaca ctctgaaccg cgcaattgca cgtggtgtgg gcggtgatga
    39841 tgatgcgaac atggaaacca tcatctacga aggttacggt cctggcggca cggcaatcat
    39901 gattgaatgt ctgtctgaca accgcaaccg taccgttgct gaagtgcgtc atgcattcag
    39961 caaatgtggc ggtaatctcg gtactgatgg ttccgtagcc tatctgttca gcaaaaaagg
    40021 cgtcatctcc ttcgagaaag gcgatgaaga caccatcatg gaagcagcac tggaagcagg
    40081 tgctgaagac gttgtgacct atgatgacgg cgcgattgat gtctacactg catgggaaga
    40141 aatgggtaaa gtgcgcgacg ctctggaagc ggcaggtctg aaagcagaca gcgcggaagt
    40201 ttccatgatc ccgtctacca aagctgatat ggatgcagaa accgcaccga aactgatgcg
    40261 tctgatcgat atgctggaag attgcgacga cgtgcaggaa gtttaccata acggtgaaat
    40321 ctctgatgag gtcgcagcga ctctctgatg aggcctgcta aacagcaaaa cggagacgcg
    40381 tgatggctat tattctcggc attgatccgg gttcgcgcgt gaccggctac ggcgtcatcc
    40441 gccaggtagg taggcaactg tcctacctgg gtagcggatg catacgcacc aaagtggatg
    40501 atttaccgtc tcgtctgaaa ctcatctatg cgggcgtgac ggaaatcatc acccagttcc
    40561 agcctgatta tttcgccatt gaacaagtct ttatggcaaa gaatgctgac tcagccctga
    40621 aactgggcca ggcgcgcggc gtggcgattg tggcggcggt gaatcaggag ttgccagtat
    40681 ttgaatacgc ggcacgtcag gtaaagcaaa cggtggtagg tattggtagt gccgaaaaaa
    40741 gccaggtgca gcatatggtc cgcaccttgc tgaaactgcc cgctaatcca caggcggatg
    40801 ccgccgatgc gctggcgatt gccatcaccc actgccacgt tagtcagaat gcgatgcaga
    40861 tgagcgaatc gcggctgaac ctggcgagag ggcgactgcg ttaagttata ccgcctcggt
    40921 cagttccggc tgaggcgttt ccgctccctc cgcatgaatc aacaccaaac ccggatgatg
    40981 ccacaaactg gctggcgtga cggttttacg ctcccacgga atagaaccca ggaaccagaa
    41041 tttccagaaa gtgagttttg cattcggatt gctatttaac aattctccaa acgtctctat
    41101 ttcacccacc ggaatacata gcgcttcttt ataaatatca aaaacaaatt ttgaacagaa
    41161 ctggcgcgaa gattcgtatt taaaaccggt gtggtaaagt ttgcgtagcc gggagggaac
    41221 ctgttcaaca attcgttgtt tttgttgttc tgtcagccca gcgtctaatc gctttatagc
    41281 atagcgttga ttagaagaac gtttaataaa acgagatagc gtggtgatgg ttgaaagggg
    41341 gacccggctt tctgcaacca ggaagtcttc accgttatga ccgataatga tcccgacgtg
    41401 attactccaa caatttgatg cagctgatat ttgaccaaat aaggcagcac ctatacatgt
    41461 aaaggcgata tctccaattt catattcagc agggtaatta atattcacgt t
//
LOCUS       DAOFQD010000026        41073 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.026, whole genome shotgun sequence.
ACCESSION   DAOFQD010000026 DAOFQD010000000
VERSION     DAOFQD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.026"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            465..1019
                     /locus_tag="QBF46_001481"
     CDS             465..1019
                     /locus_tag="QBF46_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDQ6673676.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            complement(995..1252)
                     /locus_tag="QBF46_001482"
     CDS             complement(995..1252)
                     /locus_tag="QBF46_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDQ6673677.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(1249..2067)
                     /gene="aroE"
                     /locus_tag="QBF46_001483"
     CDS             complement(1249..2067)
                     /gene="aroE"
                     /locus_tag="QBF46_001483"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDQ6673678.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            complement(2072..2644)
                     /gene="tsaC"
                     /locus_tag="QBF46_001484"
     CDS             complement(2072..2644)
                     /gene="tsaC"
                     /locus_tag="QBF46_001484"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDQ6673679.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            complement(2649..3191)
                     /locus_tag="QBF46_001485"
     CDS             complement(2649..3191)
                     /locus_tag="QBF46_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDQ6673680.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            complement(3220..3693)
                     /locus_tag="QBF46_001486"
     CDS             complement(3220..3693)
                     /locus_tag="QBF46_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDQ6673681.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            complement(3665..4789)
                     /gene="dprA"
                     /locus_tag="QBF46_001487"
     CDS             complement(3665..4789)
                     /gene="dprA"
                     /locus_tag="QBF46_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDQ6673682.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            4919..5428
                     /gene="def"
                     /locus_tag="QBF46_001488"
     CDS             4919..5428
                     /gene="def"
                     /locus_tag="QBF46_001488"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDQ6673683.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            5443..6390
                     /gene="fmt"
                     /locus_tag="QBF46_001489"
     CDS             5443..6390
                     /gene="fmt"
                     /locus_tag="QBF46_001489"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDQ6673684.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            6436..7725
                     /gene="rsmB"
                     /locus_tag="QBF46_001490"
     CDS             6436..7725
                     /gene="rsmB"
                     /locus_tag="QBF46_001490"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDQ6673685.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            7747..9123
                     /gene="trkA"
                     /locus_tag="QBF46_001491"
     CDS             7747..9123
                     /gene="trkA"
                     /locus_tag="QBF46_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDQ6673686.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            9253..9663
                     /gene="mscL"
                     /locus_tag="QBF46_001492"
     CDS             9253..9663
                     /gene="mscL"
                     /locus_tag="QBF46_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDQ6673687.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(9660..9878)
                     /gene="arfA"
                     /locus_tag="QBF46_001493"
     CDS             complement(9660..9878)
                     /gene="arfA"
                     /locus_tag="QBF46_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDQ6673688.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(9934..10359)
                     /gene="zntR"
                     /locus_tag="QBF46_001494"
     CDS             complement(9934..10359)
                     /gene="zntR"
                     /locus_tag="QBF46_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDQ6673689.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTAGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            complement(10370..10738)
                     /locus_tag="QBF46_001495"
     CDS             complement(10370..10738)
                     /locus_tag="QBF46_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDQ6673690.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            complement(10845..11228)
                     /gene="rplQ"
                     /locus_tag="QBF46_001496"
     CDS             complement(10845..11228)
                     /gene="rplQ"
                     /locus_tag="QBF46_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDQ6673691.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(11269..12258)
                     /gene="rpoA"
                     /locus_tag="QBF46_001497"
     CDS             complement(11269..12258)
                     /gene="rpoA"
                     /locus_tag="QBF46_001497"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDQ6673692.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(12284..12904)
                     /gene="rpsD"
                     /locus_tag="QBF46_001498"
     CDS             complement(12284..12904)
                     /gene="rpsD"
                     /locus_tag="QBF46_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDQ6673693.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(12938..13327)
                     /gene="rpsK"
                     /locus_tag="QBF46_001499"
     CDS             complement(12938..13327)
                     /gene="rpsK"
                     /locus_tag="QBF46_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDQ6673694.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(13344..13700)
                     /gene="rpsM"
                     /locus_tag="QBF46_001500"
     CDS             complement(13344..13700)
                     /gene="rpsM"
                     /locus_tag="QBF46_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDQ6673695.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(13847..13963)
                     /gene="rpmJ"
                     /locus_tag="QBF46_001501"
     CDS             complement(13847..13963)
                     /gene="rpmJ"
                     /locus_tag="QBF46_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDQ6673696.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(13995..15326)
                     /gene="secY"
                     /locus_tag="QBF46_001502"
     CDS             complement(13995..15326)
                     /gene="secY"
                     /locus_tag="QBF46_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDQ6673697.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(15334..15768)
                     /gene="rplO"
                     /locus_tag="QBF46_001503"
     CDS             complement(15334..15768)
                     /gene="rplO"
                     /locus_tag="QBF46_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDQ6673698.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(15772..15951)
                     /gene="rpmD"
                     /locus_tag="QBF46_001504"
     CDS             complement(15772..15951)
                     /gene="rpmD"
                     /locus_tag="QBF46_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDQ6673699.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(15955..16458)
                     /gene="rpsE"
                     /locus_tag="QBF46_001505"
     CDS             complement(15955..16458)
                     /gene="rpsE"
                     /locus_tag="QBF46_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDQ6673700.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(16473..16826)
                     /gene="rplR"
                     /locus_tag="QBF46_001506"
     CDS             complement(16473..16826)
                     /gene="rplR"
                     /locus_tag="QBF46_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDQ6673701.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(16836..17369)
                     /gene="rplF"
                     /locus_tag="QBF46_001507"
     CDS             complement(16836..17369)
                     /gene="rplF"
                     /locus_tag="QBF46_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDQ6673702.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(17382..17774)
                     /gene="rpsH"
                     /locus_tag="QBF46_001508"
     CDS             complement(17382..17774)
                     /gene="rpsH"
                     /locus_tag="QBF46_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDQ6673703.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(17808..18113)
                     /gene="rpsN"
                     /locus_tag="QBF46_001509"
     CDS             complement(17808..18113)
                     /gene="rpsN"
                     /locus_tag="QBF46_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDQ6673704.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(18128..18667)
                     /gene="rplE"
                     /locus_tag="QBF46_001510"
     CDS             complement(18128..18667)
                     /gene="rplE"
                     /locus_tag="QBF46_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDQ6673705.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(18682..18996)
                     /gene="rplX"
                     /locus_tag="QBF46_001511"
     CDS             complement(18682..18996)
                     /gene="rplX"
                     /locus_tag="QBF46_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDQ6673706.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(19007..19378)
                     /gene="rplN"
                     /locus_tag="QBF46_001512"
     CDS             complement(19007..19378)
                     /gene="rplN"
                     /locus_tag="QBF46_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDQ6673707.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(19543..19797)
                     /gene="rpsQ"
                     /locus_tag="QBF46_001513"
     CDS             complement(19543..19797)
                     /gene="rpsQ"
                     /locus_tag="QBF46_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDQ6673708.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(19797..19988)
                     /gene="rpmC"
                     /locus_tag="QBF46_001514"
     CDS             complement(19797..19988)
                     /gene="rpmC"
                     /locus_tag="QBF46_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDQ6673709.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(19988..20398)
                     /gene="rplP"
                     /locus_tag="QBF46_001515"
     CDS             complement(19988..20398)
                     /gene="rplP"
                     /locus_tag="QBF46_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDQ6673710.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(20411..21112)
                     /gene="rpsC"
                     /locus_tag="QBF46_001516"
     CDS             complement(20411..21112)
                     /gene="rpsC"
                     /locus_tag="QBF46_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDQ6673711.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(21130..21462)
                     /gene="rplV"
                     /locus_tag="QBF46_001517"
     CDS             complement(21130..21462)
                     /gene="rplV"
                     /locus_tag="QBF46_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDQ6673712.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(21477..21755)
                     /gene="rpsS"
                     /locus_tag="QBF46_001518"
     CDS             complement(21477..21755)
                     /gene="rpsS"
                     /locus_tag="QBF46_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDQ6673713.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(21772..22593)
                     /gene="rplB"
                     /locus_tag="QBF46_001519"
     CDS             complement(21772..22593)
                     /gene="rplB"
                     /locus_tag="QBF46_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDQ6673714.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(22611..22913)
                     /gene="rplW"
                     /locus_tag="QBF46_001520"
     CDS             complement(22611..22913)
                     /gene="rplW"
                     /locus_tag="QBF46_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDQ6673715.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(22910..23515)
                     /gene="rplD"
                     /locus_tag="QBF46_001521"
     CDS             complement(22910..23515)
                     /gene="rplD"
                     /locus_tag="QBF46_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDQ6673716.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(23526..24155)
                     /gene="rplC"
                     /locus_tag="QBF46_001522"
     CDS             complement(23526..24155)
                     /gene="rplC"
                     /locus_tag="QBF46_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDQ6673717.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(24188..24499)
                     /gene="rpsJ"
                     /locus_tag="QBF46_001523"
     CDS             complement(24188..24499)
                     /gene="rpsJ"
                     /locus_tag="QBF46_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDQ6673718.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            complement(24737..25156)
                     /gene="gspB"
                     /locus_tag="QBF46_001524"
     CDS             complement(24737..25156)
                     /gene="gspB"
                     /locus_tag="QBF46_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HDQ6673719.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEENTVEESEEKAGLRERVKNALNELER"
     gene            complement(25158..26627)
                     /gene="gspA"
                     /locus_tag="QBF46_001525"
     CDS             complement(25158..26627)
                     /gene="gspA"
                     /locus_tag="QBF46_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HDQ6673720.1"
                     /translation="MSTRREVILSWLCEKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            26807..27622
                     /gene="gspC"
                     /locus_tag="QBF46_001526"
     CDS             26807..27622
                     /gene="gspC"
                     /locus_tag="QBF46_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDQ6673721.1"
                     /translation="MPTLRFPFHLANHNKDAAINILIIFISIGSIIFNVNYFHTTIVK
                     NGQIINQPTNAFQSDFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVGELTGMNGLRHERTQ"
     gene            27606..29558
                     /gene="gspD"
                     /locus_tag="QBF46_001527"
     CDS             27606..29558
                     /gene="gspD"
                     /locus_tag="QBF46_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDQ6673722.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSAQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYHSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            29568..31049
                     /gene="gspE"
                     /locus_tag="QBF46_001528"
     CDS             29568..31049
                     /gene="gspE"
                     /locus_tag="QBF46_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDQ6673723.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANKDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            31046..32242
                     /gene="gspF"
                     /locus_tag="QBF46_001529"
     CDS             31046..32242
                     /gene="gspF"
                     /locus_tag="QBF46_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDQ6673724.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            32252..32689
                     /gene="gspG"
                     /locus_tag="QBF46_001530"
     CDS             32252..32689
                     /gene="gspG"
                     /locus_tag="QBF46_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDQ6673725.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     VVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            32697..33206
                     /gene="gspH"
                     /locus_tag="QBF46_001531"
     CDS             32697..33206
                     /gene="gspH"
                     /locus_tag="QBF46_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDQ6673726.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            33203..33580
                     /gene="gspI"
                     /locus_tag="QBF46_001532"
     CDS             33203..33580
                     /gene="gspI"
                     /locus_tag="QBF46_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDQ6673727.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LALWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            33573..34160
                     /gene="gspJ"
                     /locus_tag="QBF46_001533"
     CDS             33573..34160
                     /gene="gspJ"
                     /locus_tag="QBF46_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDQ6673728.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            34153..35136
                     /gene="gspK"
                     /locus_tag="QBF46_001534"
     CDS             34153..35136
                     /gene="gspK"
                     /locus_tag="QBF46_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDQ6673729.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTLSFHSQLQRTRQVKHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDSFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKRHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            35151..36314
                     /gene="gspL"
                     /locus_tag="QBF46_001535"
     CDS             35151..36314
                     /gene="gspL"
                     /locus_tag="QBF46_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDQ6673730.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFHFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            36311..36772
                     /gene="gspM"
                     /locus_tag="QBF46_001536"
     CDS             36311..36772
                     /gene="gspM"
                     /locus_tag="QBF46_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HDQ6673731.1"
                     /translation="MIKSWWAEKSTSEKKIVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQEQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQVTYGIVIEDLQFTLAGNEEITLRHLSFREQQ"
     gene            36772..37449
                     /gene="gspO"
                     /locus_tag="QBF46_001537"
     CDS             36772..37449
                     /gene="gspO"
                     /locus_tag="QBF46_001537"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HDQ6673732.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(37478..37954)
                     /gene="bfr"
                     /locus_tag="QBF46_001538"
     CDS             complement(37478..37954)
                     /gene="bfr"
                     /locus_tag="QBF46_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDQ6673733.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(38026..38220)
                     /gene="bfd"
                     /locus_tag="QBF46_001539"
     CDS             complement(38026..38220)
                     /gene="bfd"
                     /locus_tag="QBF46_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDQ6673734.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(38389..41055)
                     /gene="chiA"
                     /locus_tag="QBF46_001540"
     CDS             complement(38389..41055)
                     /gene="chiA"
                     /locus_tag="QBF46_001540"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HDQ6673735.1"
                     /translation="MIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVS
                     STNCPGKAKVNDATNPWRLKRTATAAEISQYGNTLSCEKSGSSSSSNSNTPASNTPAN
                     GGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDA
                     SNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNY
                     AVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDARNPWRYVRAATATEI
                     SQYGNPGSCSVKPDNNGGAVTPVDPTPETPVTPTPDNSEPSTPADSVNDYSLQAWSGQ
                     EGSEIYHVIFNGNVYKNAWWVGSKDCPRGTSAENSNNPWRLERTATAAELSQYGNPTT
                     CEIDNGGVIVADGFQASKAYSADSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPA
                     KAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSY
                     SNEELNNAPQFNPETLYASDTLIRFNGVNYISQSKVQKVSPSDSNPWRVFVDWTGTKE
                     RVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWG
                     TAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLN
                     LKVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGM
                     NVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLKGLH
                     PNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLP
                     GGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT        10307 a        10671 c         9708 g        10387 t
ORIGIN      
        1 gatgctcaaa gaattaaact tcgtaatgaa ttacgtgttc actcttgaga cttggtattc
       61 atttttcgtc ttgcgacgtt aagaatccgt atcttcgagt gcccacacag attgtctgat
      121 aaattgttaa agagcagttg cgacgcggct ttcagctcac tgtcgcgagg tggcgtatat
      181 tacgctttcc tctttcagag tcaaccctga atttcaggat tttttctctt cgttgttacg
      241 accatcttgt gaagtgtttc acgttgtcgt ctcaacggag gcgcattata gggatcccaa
      301 ttttttgcac aagtactttt ttgatctttt tttctgtttg ttgttttttc accctttttg
      361 ctgcattcgc acacaaaacg gtgctttttt gcatactaaa agacttgcac aaggccaata
      421 atgcccccaa agtcattagt aaatcattta ttgctgaggt aagtatgtct gatgttttac
      481 gcccataccg cgatcttttt ccacaaatcg gtcagcgcgt aatgatcgac gatagcagtg
      541 tcgtgattgg tgacgttcgt ctggctgatg atgtggggat ctggccgctc gttgtgattc
      601 gtggagatgt acattatgta cagatcggag cacgcaccaa tatccaggat ggcagtatgt
      661 tgcatgtcac tcataaatcc tcgtacaacc cagatggcaa cccattaacc attggcgaag
      721 atgtcactgt tggtcacaag gtgatgctcc acggctgcac cattggcaat cgagttttgg
      781 ttgggatggg ctcaatttta cttgatggcg caatagtaga agatgatgtg atgattggtg
      841 cgggtagtct ggtcccacaa aataaacggc tggagagcgg atatctgtat ctcggtagcc
      901 ccgtcaaaca gatccgcccg ttaagtgatg aagagaaggc tgggttacgc tattccgcga
      961 ataattacgt gaaatggaag gacgaatatc tggatcaggg taaccagacc cagccttgat
     1021 catcttcact ttgttcctga attaagtttt ccgcttcttc ttccaggtcc cagcgatgct
     1081 gacgaaaact cgctaaccac tgttctggcg tatctccagt aaagcgatac gccagactct
     1141 cgccagagat cgcgcatgtc agttgcatac cattcacgag agcgggaaaa catacacatt
     1201 ttttattctc gtcccactct tccctgtccg gaaactggat ggcctgattc acgcggacaa
     1261 ttcctcctgc aattgcttta taactggttc tacgtcaggc agaacaccgt gccagagaag
     1321 aaaggcatga gccgcctgtg ccaccagcat tcctaaacca tcagcattac gctttgagcc
     1381 tcgctgctca caccatgcca gaaaaggagt ttttcctttc tgatagaaca tgtcatagca
     1441 ataaatgcct ggatgaatga gcgatgacgg gatcgccgga atatcaccac tgatgccact
     1501 ggatgttgca ttaataatga gatcaaactc atgaccttcc agttcgtcca tacccaacgc
     1561 ctgaatactg ccagtgtgcg caaacaattt agccaactct tccgcgcggg ataccgtccg
     1621 attagtgatt gtcaccgcac agtccaggga aagaagtggc agtagtacgc cgcgagatgc
     1681 tccaccagcg ccgatgagca gaatacgtaa accaggacgg ataaaagaca gacgttccag
     1741 atcgcttaac aaacctatac catcggtatt gtcacccagc aggcgtccat cttctaaccg
     1801 cttgagggta ttaacagcac cagccaacgc tgcccgttca gtaagctcat ccgctctggc
     1861 aaaagcctct tctttaaaag gcaccgtcac attcgcacct ttaccaccag cacgaaagaa
     1921 agcgttcagt gtgttgatga aatcattgat gggtgccaac acgcgcccat agggatgttc
     1981 aatattcagt tgctgagcaa attgctgatg aatgaatggc gatttgctgt gggctatcgg
     2041 attaccaaaa acagcatagg tttccattat gttacccctg tcgaaacagt tcacccgtca
     2101 gggcatcgcg gatttctgaa ggatttaaac gcccccccgt ttcaccaggc acaaccggga
     2161 acgccgcgcc aaattgtgcg cgaacttcgt ctactgttcg acaaggtggc aatccactca
     2221 agttggcact ggtagaaacc agcggtttac cataagcctg gcacaaagca accaccaacg
     2281 gatggtcggt gactcgtaca gcaagcgaat caaagcggcc cgtcaaccag cgcggtgttg
     2341 tcgcaggcgc gggaaagaca aaggtgacag gacctggcca gcgggaaaaa atggtttcac
     2401 gctgcgcatc agtcagcatg gtgtcatcaa tatagggttt aagctgctcg taatttgccg
     2461 cgattaaaat cagcccctta tcaaccggac gctgttttaa ctccaacagt ctcatcactg
     2521 ctgtttcgct atcaggatcg cagccgacac cgaaaacagc ttccgttgga taggcgatga
     2581 cacgttcttc attgaggaca tctatcgcag ctgcgatagc gtctccttgc aggttattat
     2641 tcacgttatt attccgccga aaccggcttt ccacattgtt tactggcaca aaagtgtttt
     2701 acaccctgcg cggttttctt ttcgatgagt agcggataat gacactcagg gcactctccg
     2761 gctattggtt tgaagttaat agcaaattga cactcagggt agcgatcaca ggagtgaaat
     2821 gttttgccat aacgggagcg gcgctggacc agatggcccg tccgacattg aggacaggta
     2881 attgctgttt cgtccggttt atcgataagt tcggtatgtt cgcattctgg gtagttactg
     2941 caaccaataa acataccaaa gcgtccctgg cgtaatacca gatttgcgcc acatgcaggg
     3001 caaacctgcc cctccagaac tttgacgata tgcccatccg ctgaagattt cagaggacgg
     3061 acgtagtcac acgccggata ctgtgagcac ccaagaaacg gaccgtgttt cccggatcga
     3121 ataaccagtt cagccccgca ctttgggcag gactcattat tacgcaccgt gaacagtgct
     3181 gatttcgcca taacaactta tgctgaatta aagaaatgat taatgcagca taccttcatt
     3241 cacttcaaag agtaattctt ccatttgctg gtacgcattt tcgcagcccg gaatattgaa
     3301 caacaccatc aggattaccc atttcaggtc ttccaggtcg aactctgcgg tatccagcgc
     3361 gagcactcgc tctatcacca tttcacgggt ttcaaggtta agcacctgaa tttgctcaag
     3421 gaaaagcaga aaaccacggc agctggcatc cagtctttca cactcctcag gggtataaat
     3481 acgcatggag agtggatcag aggccagttg catcggttct gccagtcctt cctgataatc
     3541 cgcaagcttt tccagccaaa gcagggcatt gtagatatct tctcgatcaa atcctgcgtc
     3601 ggtaagatcc tgttcaagtt tgtcttgatc cacacgcaac tcagcttctg tgtgaatata
     3661 ggtttcaaac aaatacatta gtacgtcgaa catggcatgc cctcctcaat cggacatagc
     3721 cgccgggtac agctgcgatc catcctgcta actccagttc gagtagttga gtaactacct
     3781 ctggcacagg ttggccggca cgttcagcga cgacgtcaac aggtgttacc tcatctccta
     3841 cgttagccag gagctcagga aatggcaatg ccacgtcttg ctgatctggt gaataaaatg
     3901 aattttcagg ggcgtctggc aaccagtgca atccaaattg caagttttcc agaatttctt
     3961 ccggttccgt cacaagaatc gcaccttgtt ttattaacca gtgaggccct tcgcttcccg
     4021 gattccctat tggacctggc aaggcaaaaa cttcacgccc ctgctcaagc gcacaacgtg
     4081 ctgtcaccag cgaaccactg cgcaaagccg cttccaccac cagtacacct ttacttagac
     4141 cactgataat gcgatttctt cgtgggaaat tgtaagcaag gggtggaaca tcgaggggaa
     4201 attccgagac gagagctccg ccatgttcaa gcagactggc agccagtcgg gcatggcggc
     4261 ggggatgaat ggtattaagt ccattcccca atacagcaat gctgacgcca tttacctgta
     4321 aggcagcttt atgcgccaca ccatcgattc cacgcgccag tccactcgta attgtcactc
     4381 cacgcgtcgc cagagtttcg caaaataacc gtccccatcg ctcgccatac catgaatgcg
     4441 cccgactccc cactacggca agctgaaatg aatgcagcgc gtgcagttct ccttcaacaa
     4501 acagtgcgcc ggggtaatct gtcgtcacca gaagttgagg aggataaaat tcgctgtccg
     4561 cagggattaa atgatggttg ggttgctcca accaacaaag tgagctttcg atactctttc
     4621 gcggaaatga aagaaagcgt tgtgcctgcc gcaatgtaag ccctgtttgc tgcaatccaa
     4681 ccgcatcaat ttgcgactgt tttgccagcc agtgagctat acggaccata tcatcgccgt
     4741 acaagctgct gatactcatt aaacgcagcc aaatttctgt atcgaccatc cttatctccc
     4801 tgccataagc agccttagca atctttgcga ttggtcagtg atgctgtcaa tcagaggggg
     4861 atttgtctag aatagaagaa ataatctttc taactcctga acacatctct ggagatttat
     4921 gtcagttttg caagtgttac atattccgga cgagcggctt cgcaaagttg ctaaaccggt
     4981 agaagaagtg aatgcagaaa ttcagcgtat cgttgatgat atgttcgaga cgatgtacgc
     5041 agaagaaggt attggcctgg cggcaaccca ggttgatatc catcaacgta tcattgttat
     5101 tgatgtttcg gaaaaccgtg acgaacggct ggtgttaatc aatccggagc ttttagaaaa
     5161 aagcggcgaa acaggcattg aagaaggttg cctgtcgatc cctgaacaac gtgctttagt
     5221 gccacgcgca gagaaagtta aaattcgcgc cctggaccgc gacggtaaac catttgaatt
     5281 ggaagcagac ggtctgttag ccatctgtat tcagcatgag atggatcacc tggtcggcaa
     5341 actgtttatg gattatctgt caccgttgaa acaacaacgt attcgtcaga aagttgaaaa
     5401 actggatcgt ctgaaagccc gggcttaagg ataagaacta acgtgtcaga atcactacgt
     5461 attatttttg cgggtacacc tgactttgca gcgcgtcatc tcgacgcgct gttgtcttct
     5521 ggtcataacg tcgttggcgt gttcacccag ccagaccgac cggcaggacg cggtaaaaaa
     5581 ctgatgccca gcccggttaa agttctggct gaggaaaaag gtctgcccgt ttttcaacct
     5641 gtttccctgc gtccacaaga aaaccagcaa ctggtcgccg atctgcaggc tgatgttatg
     5701 gtcgtcgtcg cctatggctt aattctgcct aaagcagtgc tggagatgcc gcgtcttggc
     5761 tgtatcaacg ttcatggttc actgctgcca cgctggcgcg gtgctgcacc aatccaacgc
     5821 tcactatggg cgggtgatgc agaaactggt gtgaccatta tgcaaatgga tgtcggttta
     5881 gacaccggtg acatgctcta taagctctcc tgcccgatta ctgcagaaga taccagtggt
     5941 acgctgtacg acaagctggc agagcttggc ccacaagggc ttatcaccac gttgaaacaa
     6001 ctggcagacg gcacggcgaa accagaagtt caggacgaaa ctcttgtcac ttacgccgaa
     6061 aagttgagta aagaagaagc gcgtattgac tggtcacttt cggcagcaca gcttgaacgc
     6121 tgcattcgcg ctttcaatcc atggccaatg agctggctgg aaattgaagg acagccggtt
     6181 aaagtctgga aagcatcggt cattgatacg gcaaccaacg ctgcaccagg aacgatcctt
     6241 gaagccaaca aacaaggcat tcaggttgcg actggtgatg gcatcctgaa cctgctctcg
     6301 ttacaacctg cgggtaagaa agcaatgagc gcgcaagacc tcctgaactc tcgtcgggaa
     6361 tggtttgttc cgggcaaccg tctggtctga tagtccactc ttctaagccc ggtcttgccg
     6421 ggcgttttta tacttatgaa aaaacaacgt aatttacgta gcatggcggc ccaggccgtt
     6481 gaacaagtcg tcgagcaagg gcaatcatta agcaacattc tgccaccgct ccagcaaaaa
     6541 gtttccgata aagacaaagc acttcttcaa gagttgtgct ttggcgtact gcgtacgctt
     6601 tcgcagttag actggctgat taataagtta atggcccgtc cgatgaccgg caaacagcgg
     6661 accgtgcatt acctgattat ggttggtttg tatcaactgc tttatacccg cattccacct
     6721 catgctgcgc tggctgaaac ggttgaaggc gctatcgcaa ttaagcgtcc gcaacttaaa
     6781 gggttgataa acggtgtatt acgccagttc cagcgtcagc aagaagagtt attagccgag
     6841 tttaatgcca gtgatgcacg ttatctgcat ccttcctggt tgctgaagcg tctgcaaaaa
     6901 gcgtatccag agcagtggca atccatcgtc gaagctaata accagcgtcc gccaatgtgg
     6961 ctgcgcgtta atcgtacgca tcattcccgc gacagctggc ttgcattgtt ggatgaagca
     7021 ggaatgaaag gtttcccgca tgcggattac cctgatgctg tacggctgga aacacctgca
     7081 cctgttcatg cgctacctgg ttttgaagac ggatgggtta ccgttcagga tgcatcagca
     7141 caaggttgca tgacctggct tgcgccacaa aacggtgaac acattctgga tctttgtgcc
     7201 gcccccggcg gtaaaacaac gcatatcctt gaggtggcac cagaagcgca ggttgttgcg
     7261 gttgatattg acgaacagcg cctctctcgc gtttacgaca atttaaaacg ccttggtatg
     7321 aaagcgaccg tgaaacaagg tgatggccgc tacccttccc aatggtgtgg cgagcaacag
     7381 tttgatcgca ttttattaga tgcgccttgt tcagcaaccg gtgtgattcg tcgccatccg
     7441 gatattaaat ggttacgtcg cgatcgcgat atcccggaac tcgcgcaatt gcagtctgaa
     7501 attctcgacg ccatttggcc gcatttaaaa tccggtggaa ccctggtcta tgccacctgt
     7561 tcggtgttac cggaagagaa tagcctgcag attaaagcct ttttgcaacg taccgctgat
     7621 gccgaacttt gcgaaacagg aacaccagag caaccgggta aacaaaatct gcctggtgcc
     7681 gaagagggcg acggcttctt ttacgctaag ctaatcaaaa agtgatgaga taacgggtcg
     7741 cgactgatga aaattatcat tctgggtgcc ggccaggttg gcggcacact ggcggaaaac
     7801 ctggttggcg agaacaacga tattactgtt gtcgatacca acggtgagcg tctgcggacc
     7861 ttgcaggata aatttgacct gcgagtcgtg caggggcatg gctctcatcc acgcgtattg
     7921 cgggaggcag gtgccgacga cgccgatatg ctggttgctg taaccagttc agatgaaacc
     7981 aatatggttg cctgccaggt agcctactca cttttcaaca cccctaatcg catcgctcgt
     8041 atccgctcac cagactacgt gcgcgatgcc gataagctat ttcattcaga tgctgtaccg
     8101 attgatcatc tgatcgcacc agagcagttg gttatcgata atatttaccg actgattgag
     8161 tatcccggcg cattgcaggt ggtgaacttc gctgagggta aagtcagcct cgctgtggtt
     8221 aaagcctatt atggcggccc gctgattggt aatgcacttt cgaccatgcg cgaacatatg
     8281 ccacatatcg atactcgtgt ggcagcaatt ttccgccacg atcgccctat tcgtccgcaa
     8341 ggttcgacca ttgttgaagc gggtgatgaa gtgttcttta ttgccgcttc acagcatatc
     8401 cgcgcggtga tgagtgaatt acagcgactg gaaaaaccgt ataagcggat catgctggtt
     8461 ggtggcggta atatcggtgc agggctggcg cgtcgtctgg aaaaagatta cagcgttaaa
     8521 ctcatcgaac gtaatcagca gcgcgctgcc gaactggcgg aaaagttgca gaatacgatc
     8581 gtcttttttg gtgatgcgtc ggatcaagaa ctgctggccg aagaacatat cgatcaagtt
     8641 gatctgttta ttgccgtcac caacgatgac gaggccaata tcatgtccgc catgcttgcc
     8701 aaacgtatgg gtgcgaaaaa ggtgatggta ttgatccagc gtcgcgctta tgtggatctg
     8761 gttcagggga gcgtgatcga tattgcaatt tcaccacaac aagcaactat ttctgcgttg
     8821 cttagccatg tgcgaaaagc agatattgtt ggtgtttcct cattgcgccg cggcgtagca
     8881 gaagctattg aagccgttgc tcacggtgat gaaagcacct cacgcgttgt cggcagagtc
     8941 attgacgaaa tcaagctacc gccaggaacg attattggag cggtggtacg tggaaacgac
     9001 gtgatgattg ccaatgacaa tctgcgcatt gagcaaggcg atcacgtaat tatgttcctc
     9061 acagataaaa agtttattac cgacgtcgaa agactcttcc agccaagccc tttcttcttg
     9121 taattaataa ggcgtctaat gacgccttat tatttccctt tgattatcaa ggattaatta
     9181 aattcattcc tggcaggaaa atggcttaac atttgttaga cttatggttg tcggcttcat
     9241 agggagaata acatgagcat tattaaagaa tttcgcgaat ttgcgatgcg cgggaacgtg
     9301 gtggatttgg cggtgggtgt cattatcggt gcggcattcg ggaagattgt atcctcactg
     9361 gttgccgata tcatcatgcc acctctgggc ttattaattg gcgggatcga ctttaaacag
     9421 tttgctgtca cgctacgcga tgcgcagggg gatatccctg ctgttgtgat gcattacggt
     9481 gtcttcattc aaaacgtctt tgattttctg attgtggcct ttgccatctt tatggcgatt
     9541 aagctaatca acaaactgaa tcggaaaaaa gaagaaccag cagccgcacc agcaccaact
     9601 aaagaagaag tattactgac agaaattcgt gatttgctga aagagcagaa taaccgctct
     9661 taacaaacgc ctgaaagcag aaggccagtg gtaaaaaagt gatttacttt cttgccactg
     9721 gcctcccagt tcccccgatt gccatgtttt ccttttcgca tgtaactgcc tttccctttc
     9781 ttatttttct ctactcgctg tcggaataag ggatcatgta gtaatgcttc tatcgcatta
     9841 tcctttatct gccctttagt atgctgatat cggctcataa aaactccagt tggttattta
     9901 acggcgcgag tgtaatcctg tcagtgcaaa aaatcaacag ccactcttaa cgccactcgc
     9961 cccttgttca agagcttcaa gaatcgagca ataaacactg ctatgggcgg tcccacaaca
    10021 ggcatcgtta aggcgttgca aggaacgctg catactctgc aactcggcta tccgtgcttc
    10081 gacttcctgc aatctttcct gcacaatgcc ttttgactcc tgacaggtat ggtgttcagg
    10141 atcgatacgg atcgacagca actcgcggat cgactccaga ctgaaaccta gttgtctggc
    10201 atggcggata aatttcaatc gctggagatc gctttcggta tacaggcgaa acccacctgc
    10261 agtacgcact tcatgctcca tcatctgctg tttttcgtaa taacgaatcg tgtcgggtgt
    10321 tacttccgcc atttttgcca gctcaccaat gcgatacata catactccac tagttatcgt
    10381 tgattttatt caacaacttg tcagcataat cgccgcgtaa aaaatcggta ctcagtccag
    10441 cttgtcgcag cttcaattcc agtaatgaca atcgacggct aacctcttga tattgcggat
    10501 catcgtgacg gatacctttg agaatatcca gaagctgaat tgcttccctc cgttgctcaa
    10561 gttctggcgg taagcaaccg gcattcttca acaagcgata ccccgcacgt aattccggtg
    10621 gcacgtgaga atcatcatcc agtatcaatg gttcgccgct acctgccagg ttatcaaact
    10681 cacctttcgc ttgcgcttct gctatatgac gctctgccca ctggtcaagt aaccacatat
    10741 atactccagg ggatgaacaa aaagagtaca gctattgtag ataagtgggg atgctacggg
    10801 tataaaaaaa cccgccgggg cgggtttttt tacgttgctt cagattactc tgcagcagct
    10861 tctgctttct ctgaacgatc aaccagctcg atgtaagcca tcggcgcgtt gtcgcctgca
    10921 cggaagccac acttcagaat acgagtgtaa ccaccggcac ggctcgcgaa acgcgggccc
    10981 agttcgttaa acagttttgc cacgatctcg ttatcacgag tacgggcgaa tgccagacga
    11041 cgattagcaa cgctatcagt cttggcaaga gtaatcagcg gctcaactac gcggcgcagc
    11101 tctttcgctt taggcagagt cgtcttgatg atttcatgac gaaccagtga acctgccata
    11161 ttgcggaaca tagcctggcg atggctgctg ttgcggttca gttgacgacc actcttacga
    11221 tggcgcatga ccttatcctt ctcagtaaaa ccttaacctg tgatccggtt actcgtcagc
    11281 gatgcttgcc ggtggccagt tttccaggcg catgcccaga gacagtccac gggaagccag
    11341 cacgtcttta atctcagtaa gagatttttt accaaggtta ggcgttttaa ggagctcaac
    11401 ctcggtacgc tgtaccagat caccgatata gtggatagct tctgctttaa ggcagttagc
    11461 agagcggaca gtcaattcca gatcgtcaac agggcgcagc aggatcggat cgaactctgg
    11521 tttctcttct ttcacttcag gctgacgtac atcacgtaag tcaacgaaag cttccagttg
    11581 ttcagccaga atggttgccg cacgacgaat cgcctcttca ggatcgattg tgccgttggt
    11641 ttccatttcg atgaccagct tgtccaggtc ggtacgctgt tctacacgcg ctgcttcaac
    11701 attgtaggca atacgctcca cagggctgta gcatgcgtcg accagcagac ggccgattgg
    11761 gcgctcatct tcttccgaat gaattcgggt agaagccggc acataaccac gaccgcgctg
    11821 aactttgata cgcatgctaa tagacgcgtt ctcatcggtc aggtggcaga tcacgtgctg
    11881 cggcttgacg atttcgacat caccgtcgtg ggtgatatcg gctgcagtca cagggccaat
    11941 gccagattta ttcaaggtaa gaataacttc atctttgccc tgaactctca ccgccagccc
    12001 tttcaggttg agcaggattt ccaggatatc ttcctgaacg ccttctttgg tgctgtactc
    12061 atgtagtaca ccatcaatct caacctcggt caccgcgcaa cccggcatcg atgagagcag
    12121 aatacggcgc agtgcgttac ccagagtatg gccaaagcca cgctctaaag gctcaagggt
    12181 caccttggcg tgcgtcgaac tcacttgctc gatatcaacc aggcgcggtt ttagaaactc
    12241 tgtcacagaa ccctgcattg tgtcctctct ttggtactaa gctttacttg gagtaaagct
    12301 cgacgatcag gtgttcgtta atgtccgcag acagatcaga acgctccggc ttacgcttaa
    12361 acgtaccttc catcttgcca gcatcaactt ccagccaggt tggcttttca cgctgctcag
    12421 ccagctccag agcggctttc acgcgagact gcttcttcgc tttctcacga atgcttacaa
    12481 cgtcattcgg actaacctga taagaagcga tgttaacaac acgaccgttt accataattg
    12541 ctttatggct aaccagctga cgtgcttctg cacgagtggc accgaagccc atacggtata
    12601 caacgttgtc cagacgacct tccagcagag ccaacaggtt ttcaccggtg ttgcctttca
    12661 gacgtgctgc ttctttgtag tagttacgga actgacgctc cagcacacca tagatacggc
    12721 gaactttttg cttttcacgc aactgcacac catagtcaga cagacgcggt ttacgcgcac
    12781 cgtgctggcc aggagcttgt tcaattttac acttggtatc gatcgcgcga acgccagact
    12841 taaggaataa gtcggtgccc tcacgacggc tcagcttgag cttaggaccc aaatatcttg
    12901 ccattttctt tctccaacaa acctggaaaa cgaggcgtta tacgcgacgt tttttcggcg
    12961 gacgacaacc gttatgaggg atcggagtca catcagtaat gttagtgatg cggaaacctg
    13021 cggcgttcag agcacgaata gtagattcgc ggcctggacc cggaccttta accataactt
    13081 ccagattctt gatgccgtat tctttcacgg cgtcagcgca acgctctgct gcaacctgag
    13141 ctgcaaacgg agtggatttg cgagaaccac ggaaaccgga accaccggct gttgcccaac
    13201 ccaacgcgtt accctgacga tcagtgatag tcacgatggt gttgttgaaa gaagcatgga
    13261 tatgagccac gccgtcagag acttgttttc ttacacgttt acgtgcacga attggtgcct
    13321 ttgccattat tcaatcaccc cgattatttc ttgatcggtt tgcgcggacc cttacgggta
    13381 cgtgcgttgg tcttggtacg ctgaccgcga accgggagac cacgacgatg acgcaaaccg
    13441 cgatagcaac caagatccat caggcgcttg atgctcatgc tgatttcacg gcgcagatca
    13501 ccttcaacga caaatttggc aacttcgtca cgcagcgtgt cgatttgtcc ttcagacagc
    13561 tcactgatct taacatcttc agcgataccc gctgcagcca ggatggcttt agaacgggtc
    13621 ttgccgacgc cataaatcga agttaatgcg attacggcat gcttatgatc aggaatgtta
    13681 atgcctgcta tacgggccac tatgcactcc tactatttaa tatgtacgtt ccatgctgaa
    13741 aagcccgttt tcaggatact caaatggaaa cgcacagaca tacaaaagat tggctggcta
    13801 atctagccag ctcaacccaa ctttgcaaga aaaatatgcg aaaaaatcag ccttggcgct
    13861 gtttatgctt cggctcggca ctgcaaatca cacggatgac accatcacgc ttaacgattt
    13921 tgcagttacg gcataatttc ttgacggaag cacgaacttt catttttact ctccgtaact
    13981 tctcgggcga ccaattatcg gccgtagcct ttcaggttcg ccttcttcaa tgcagactca
    14041 tactgactgg acatcatcag agtttgcact tgagccataa agtccataat cacgacaaca
    14101 acgataagca gtgaggtccc accgaagtag aacggtactt tcattgcatc acgcatgaac
    14161 tccgggatca ggcagataaa ggtaatatac agcgcaccaa ccagggtcag gcgggtcatt
    14221 actttatcga tatacttcgc cgtttgctct cccggacgaa ttcctggtac aaatgcaccg
    14281 gacttcttca ggttatctgc tgtttcacgc gggttgaaaa ccaacgccgt gtagaagaaa
    14341 cagaagaaga tgattgcaga cgcatagagt aacacataaa gcggttgccc aggctgcaaa
    14401 tacagcgaaa ttgttgtcag ccagttccaa ccagtaccgc ccccgaacca tgacgcgatg
    14461 gtcgccggga acagaataat actggaagcg aagattgccg ggattacccc cgccatattc
    14521 actttcagcg gtaaatgtgt gctctgtgca gcatagacac gacgaccttg ctgacgtttc
    14581 gcgtagttta ccacaatgcg gcgttgacca cgctcaacaa atacaacaaa gaacgtcact
    14641 gcaaatacta atactgcaac caacagcaac acgaggaagt gcaggtcgcc ttgacgcgct
    14701 tgctcgatag tatgggcaat ggctggcggg agtcccgcga caataccggc gaagataatg
    14761 attgaaatac cgttgccgat acctcgttca gtaatctgtt cgcccaacca catcaggaac
    14821 atcgttcctg tgaccagact tacaacagcg gtgaagtaga atgcaaagcc tgggttaatc
    14881 accaggcctt gcataccagg catattcggc agaccggtag caataccgat cgactggaat
    14941 attgccagca ccagagtacc gtaacgggtg tactggctga tcttacgacg accagactcc
    15001 ccttctttct taatttctgc caacgttggg tgaaccaccg tcagcagctg gataatgatc
    15061 gacgccgaaa tatacggcat gatccccaga gcaaagatag aagcacggct gagagcacca
    15121 ccagagaaca tgttaaacat ctcaatgatg gtgcctcgct gttgctcaag cagtttggca
    15181 agtacagcgg catcaatacc agggatcgga ataaaagagc caatacggaa cacaatcagc
    15241 gcaccgataa caaacagcag tctgcgtttc agctcgccta agccaccttt ggcactttga
    15301 aaatctaatc ccggttgttt agccatctgc tacttattcc tcgattttac cgccagcagc
    15361 ttcgatagca gcacgagcgc ctttagtaac acgcaggcca cgaacagtta ccggagtcgt
    15421 tacttcgcca gccaggatca ctttcgcgaa ctcgatctgg ataccgataa tgttagccgc
    15481 tttcagcgtg ttcaggtcta ctacaccgcc ttctacttta gccaggtcag acagacgaac
    15541 ttcggctgta atcgctgctt tacgagaagt gaagccgaat ttcggcagac gacggtacag
    15601 aggcatctga ccaccctcga aaccgcgacg tacgccaccg ccagaacgag acttctgacc
    15661 tttgtgacca cgaccaccgg ttttaccgag gccagaaccg ataccacgac ccaggcgttt
    15721 acccgccttt ttggagcctt cggccggaga cagagtattt aaacgcatct cttactcctc
    15781 aactttaacc atgaaggaaa ccgcgttgat cataccgcga atagcaggag tatcctcgcg
    15841 ctctacggtg tgaccaatac gacgcagacc caggccaagc agcgttgcct tgtgtttcgg
    15901 cagacgaccg attgcactgc gggtttgagt aattttaata gtctttgcca tggtttattt
    15961 ccccagaatt tcttcaacgg atttaccacg cttggcagcg accatttctg gagaattcat
    16021 attttccagg ccatcaatag ttgcacgaac cacgttgatc gggttggtgg aaccatatgc
    16081 tttagccaga acgttatgaa ccccagcgac ttccagaacg gcgcgcattg caccaccggc
    16141 gatgataccg gtaccttcgg aagccggctg catgaatacg cgagaacccg tgtgaacacc
    16201 tttaacaggg tgttgcagag tgccgttatt cagcgcgacg ttaatcatat tgcgacgggc
    16261 tttttccatc gctttctgga tcgctgctgg aacttcacgc gctttaccgt aaccaaaacc
    16321 aacgcgaccg ttaccatcgc caactacagt cagagctgtg aaggagaaaa tacgaccacc
    16381 tttaacggtt ttagatacgc ggtttaccgc gatcagcttt tcctgcagtt cgccagcttg
    16441 tttttcgatg tgagccatct tacacctcta ccttagaact gaaggccagc ttcacgggca
    16501 gcatctgcca gtgcctggac acgaccatga tattggaacc cggaacggtc aaaggataca
    16561 tctttgatgc ctttttccag agcgcgttca gcgacagctt tacccacagc tgcagccgcg
    16621 tctttgttac cggtgtactt cagttgttca gcgatagctt tttctacagt agaagcagct
    16681 accagaactt cagaaccgtt cggtgcaatt acctgtgcgt aaatgtgacg cggggtacga
    16741 tgtaccacca ggcgagttgc gcccagctcc tggagcttgc ggcgtgcgcg ggtcgcacga
    16801 cggatacgag cagatttctt atccatagtg ttaccttact tcttcttagc ctctttggta
    16861 cgcacgactt cgtcggcgta acgaacaccc ttgcctttat aaggctcagg acgacggtag
    16921 gcgcgcagat ccgctgcaac ctggccgatc acctgcttat cagcgccttt cagcacgatt
    16981 tcagtctgag tcggacattc agcagtgata cccgcaggca gctgatggtc aacaggatga
    17041 gagaaaccca gagacaggtt aatcacatta cctttaaccg ctgcacggta acctacacca
    17101 accagctgca gcttcttagt gaagccttcg gtaacaccga taaccattga gttcagcagg
    17161 gcacgcgcgg taccagcctg tgcccaaccg tctgcgtaac catcacgcgg accgaaggtc
    17221 agggtattat ctgcatgttt aacttcaaca gcatcgttga gagtacgagt cagctcgccg
    17281 tttttacctt tgatcgtaat aacctgaccg ttgattttta cgtcaacgcc ggcaggaaca
    17341 acgaccggtg ctttagcaac acgagacatt ttttcctccg attaggctac gtagcagata
    17401 atttcgccac caagaccagc ctggcgcgct gcacgatcag tcataacacc tttagaggta
    17461 gaaacaactg cgatacccag acccgccata actttcggca gctcatcttt acgtttatag
    17521 atgcgcagac ctgggcggct gacacgctga atgctttcta caacagcttt gccctggaaa
    17581 tacttcagag taagttccag ttcaggcttg gtgtcgcctt caactttaaa atcttcaata
    17641 aaaccttctt ccttcagcac gttggcgatt gccactttca gcttggagga aggcatggtg
    17701 accgcagctt tgttcgcggc ctgaccgtta cggatacggg tcagcatatc cgcgatcgga
    17761 tcttgcatgc tcatctgtct ttactcccgt gattcaattg gtgacaatta ccagctagcc
    17821 tttttcagac ccgggatttc accgcgcata gcggcttcac ggaccttaat acggctcaac
    17881 ccgaacttcc gcaggaaacc atgcggacga cctgtttgac ggcagcggtt acgctgacga
    17941 gacgggctgg aatcacgcgg cagagtctgc agcttgagaa cagcgttcca acgatcttcg
    18001 tcggaagcgt tcacatcaga gatgatcgct ttcagttcag cgcgtttcgc gaagtattta
    18061 tcagctaaag ctacgcgttt tacttcgcgt gctttcattg attgcttagc catttagtaa
    18121 ccctacctta cttgcggaac gggaagtcaa aggcagccag cagagcgcgg ccttcttcgt
    18181 cagatttcgc agtagtggta atggtaatat ccaaaccacg aacgcggtcg actttatcgt
    18241 agtcgatttc tgggaagatg atctgctcac ggacacccat gctgtagtta ccacgaccgt
    18301 cgaaagactt agcggacagg ccacggaagt cacggatacg aggtacagca atagtgatca
    18361 ggcgctcaaa gaactcccac atgcgttcgc cacgcagagt tactttacag ccgatcggat
    18421 agccctgacg gattttgaag cctgcaacag atttgcgtgc tttggtgatc agcggttttt
    18481 gaccggagat tgctgccagg tctgctgctg cgttatccag cagttttttg tcagcgatcg
    18541 cttcaccaac acccatgttc agggtgatct tctcgacccg agggacttgc atgacagaat
    18601 tgtagttaaa ctcagtcatg agttttttaa ctacttcgtc tttgtagtaa tcatgcagtt
    18661 tcgccatcgt actactccaa attacttgat agtttcgctg ttagacttga agaaacggac
    18721 ttttttaccg tcttcgaatc taaagcctac acggtcagcc ttgccggttg ccgcattgaa
    18781 gattgctacg ttggaaacct gaatagcggc ttctttttca acgatgccac ccggttggtt
    18841 cagggccgga accggcttct gatgtttctt aaccaggttg ataccttcaa caatgacctt
    18901 gccggaagac aggacattct taactttacc gcgtttacct ttatctttac cggttaacac
    18961 gataacttcg tcatcacgac ggattttcgc tgccatgatt cgctccttag agtacttctg
    19021 gtgccagaga gataattttc atgaacttct cactacgaag ctcacgagtt accggcccaa
    19081 aaatacgcgt accgataggc tgctcgctgt tgttgttcag aagaacacaa gcattaccat
    19141 cgaagcgaat gacagaaccg tccgggcgac gaacaccctt cttggtgcgc accactaccg
    19201 ccttcagcac atcacctttt ttgaccttac cacgcggaat tgcttctttg atggtgatct
    19261 tgatgatgtc gcctacgcct gcgtagcgac ggtgcgagcc acccagaacc ttgatacaca
    19321 ttacgcgacg tgcaccggag ttgtcggcga cgttcagcat agtctgttct tggatcattt
    19381 tagtgctccg ctaatgtcaa ctactactga gacccgaaaa tcaggtcgtt aaaaatcccc
    19441 atatcgaggg cgcggcatta taacaccgct tcaaggatat gggtagaaaa aataaacggc
    19501 tcatttctga gccgtttatt cgtattgaga gagtgtactg tattacagaa ccgctttctc
    19561 tacaacgcga accagcgtcc aggatttagt cttggacagc ggacggcatt cgcggatttc
    19621 aaccacgtca ccgataccgc attcgttgtt ctcgtcatgt acgtgcagtt tggtcgtacg
    19681 cttgatgaat ttaccgtaga tcgggtgttt cacaaaacgt tcgatagcaa caacaatgga
    19741 tttctccatt ttgtcgctaa caacgcgacc ttgcagagta cggattttat cggtcattac
    19801 gcacccgcct tctcgttcag taaagtctta acgcgtgcga catcgcgacg cacttgcttc
    19861 aacaggtgag actgttgcag ctggccactt gcagcctgca tacgcaggtt gaactgctca
    19921 cgcagcaggt tcagcagctc ggtgttcagc tcttcaacgc tcttctcacg cagctctttt
    19981 gctttcatta catcaccgtc ttagttacaa aggtggtttt aatcggcagt ttcgctgctg
    20041 ccagcttgaa tgcttcacgg gccagctctt ccggaacacc gtccatttca tacaggactt
    20101 tacccggctg aatcaaggca acccaatact ccacgttacc tttaccttta cccatacgca
    20161 ctgccagcgg cttttcagtg atcggtttgt ccgggaacac acggatccag atcttacctt
    20221 gacgcttaac tgcacgggtc atagcacgac gtgctgcttc gatctgacgg gcagtcagac
    20281 gaccacggcc aacagctttc agaccgaagc tgccgaagct aacatccgta ccctgcgcca
    20341 gaccgcggtt acggcctttg tgcattttac ggaattttgt acgctttggt tgtaacatca
    20401 gcgacgctcc ttatttacgg cctttacgct gctgcttttt aggctgagca gccggttttt
    20461 ccggttgttc aacagcagcc ataccaccca ggatctcgcc tttgaagatc cacactttaa
    20521 cgccgattac accgtaagtg gtgtgcgctt cagaggtgtt gtagtcgatg tcagcacgca
    20581 gagtgtgcag cggtacgcga ccttcgcggt accattcggt acgtgcgatt tccgcgccgc
    20641 ccagacggcc gctaacttca actttaatac ctttagcgcc cagacgcatt gcgttctgta
    20701 cagcacgctt catagcacga cggaacataa cgcgacgttc cagctgagaa gtgatgctgt
    20761 cagcaaccag ttttgcgtcc agttcaggct tacgaacttc ggcgatgttg atctgtgcag
    20821 gaacgccagc gatgtccgct acgaccttac gcagtttttc tacgtcttca ccttttttac
    20881 cgataacgat acccgggcga gcagtgtgaa tggttacacg gatgctctta gccggacgct
    20941 cgataacgat acgagatacg gacgctttag ccagttcctt agtcaggtac tgacgtactt
    21001 taaaatcgct gtccaggttg tcagcgaatt ctttggtgtt cgcaaaccag gtagagttcc
    21061 atggttttac aatacccagg cgaataccat taggatgtac tttctgaccc attgctagtc
    21121 tccagagtct cagcgatcgg acacaaccac agtgatgtgg ctggtgcgct tcaggatgcg
    21181 atctgcacga ccttttgcac gcggcataat gcgcttcatg ctcgggcctt cgtctacgaa
    21241 aattttcgta actttcagat cgtcaatgtc agcgccatcg ttgtgttcag cgttagcaat
    21301 ggcagattcc agaactttct tgaccagtac agccgctttc ttgttggtgt aggtcaaaat
    21361 atccagagcc tgcgacactt tcttaccgcg aatcaggtca gcaacaaggc gaaccttctg
    21421 agcagaagaa cgagcatggc gatgtttagc gatagtttcc atctcttcct cctaccttat
    21481 ttcttcttcg cttttttatc agcagcgtgg ccgcgataag tacgagtcgg tgcgaattca
    21541 cccagtttgt gaccaaccat ttcgtcggtt acaaataccg gaacgtgctg acgaccatta
    21601 tggacagcga tggtcaaacc gatcatgtta ggaaagatcg ttgaacgacg ggaccaagtg
    21661 cgcaggggct tcttgtctcc gctttccacc gctttctcta ccttcttcag caagtgcagg
    21721 tcaataaaag gacctttctt gagagaacgt ggcatggctt atcctctaaa attatttgct
    21781 acggcgacgt acgatgaatt tatcagtacg cttgttgctg cgggtcttct tacctttggt
    21841 ctgaacgccc cacggagtta ccgggtgctt accaaagtta cgaccttcac caccaccatg
    21901 tgggtggtct accgggttca tcgcggtacc gcgaacggtc ggacgaacac cacgccagcg
    21961 tgcagcacct gctttaccca gaacgcgcag catatgctca gcattgccaa cttcgcccag
    22021 agttgcacgg cagtctgctt ctactttacg catttcacca gaacgcagac gcagggtgac
    22081 ataagcacca tcacgagcaa cgatctgaac gtaagtacca gcggaacgtg ccagctgacc
    22141 gcctttacct ggtttcattt ctacgttatg aacagtagaa ccaaccggga tgttgcgcat
    22201 cggcagggtg ttacctggtt tgattgcagc atcaacgcca gactgaatct ggtcgccagc
    22261 tttcaggcct ttaggggcca ggatgtaacg gcgttcaccg tctttgtaca gaaccagcgc
    22321 gatgttcgcg gaacggttcg gatcgtactc aagacgttca acaactgccg ggataccgtc
    22381 tttgttgcgt ttgaagtcaa caatacggta agcctgcttg tggccaccac cgatatgacg
    22441 agtggtgata cggccattgt tgttacgacc accggatttg ctgttttttt ccagcaacgg
    22501 agcaaaaggt ttgcccttgt gcagctcagg gttaaccact ttaactacgt ggcgacgacc
    22561 cggagatgtc ggtttacatt taacaactgc cattgtatta ctcctccgac ttactcagcg
    22621 ccgccaacga agtccagatt ctggccttct ttcagggtga cgtaagcttt tttccagtcg
    22681 ctacgacgac cgatacgctg tccgtgacgt ttaactttcc ctttaactac cagggtgtta
    22741 acgacttcga cttcgacttc aaacagtttc tgcacagcag ctttgatttc tgctttggtc
    22801 gcgtctttag caactttgag tacgatggtg ttggattttt ccatcgcagt agacgctttt
    22861 tcagaaacgt gcggtgcacg cagcaccttc agcagacgtt cttcacgaat catgccagca
    22921 tctcctcaac ttgcttaaca gcatcagcag tcattacgac tttgtcgaag gcgatcaggc
    22981 taaccgggtc gataccagtt gcatcgcgta cgtcaacctt gtgcaggttg cgcgcagcca
    23041 ggaacaggtt ttcgtccagc tcaccggtga tgatcagcac atcttccaga gccatgtctt
    23101 tcagtttctg tgccagcagc ttagttttcg gcgcttctac agagaacttc tcgacaacga
    23161 tcagacgatc ctgacgtacc agttcggaca ggatgctttt cagcgcgccg cggtacatct
    23221 tcttgttaac tttttgactg tggtcctgcg gacgagcagc aaaggtcacg ccaccagaac
    23281 gccagatcgg gctcttgata gaaccagaac gcgcacggcc ggtgcctttc tggcgccacg
    23341 gttttttacc ggaaccagtt acttcagcac gagtcttctg agcacgagta ccctgacgag
    23401 caccagctgc ataagcaaca acaacctggt gaaccagcgc ttcgttgaaa tcacgaccga
    23461 aggtagtttc ggaaacagtc agcgcgctct gcgcgtcttt caatactaat tccattgcta
    23521 tctccttacg ccttcacagc tggtttaacg atcaggtcgc taccggttgc acccgggaca
    23581 gcacctttaa ccagcagcag gttgcgctca gcgtcaacgc gtactacgtc aaggctctga
    23641 acggttacac gttcgttacc catctgacct gccattttct tgcctttgaa cactttgccc
    23701 ggagtctggt tctgaccgat agaacccgga acgcggtgag acaaggagtt accgtgagta
    23761 gcgtcctggg tacggaagtt ccagcgctta acggtacctg cgaaaccttt acctttagag
    23821 gtgccagtta cgtcaacttt tttaacgtca gcaaacagtt caacgctaat gctctgacct
    23881 acagtgaact cttcgccttc agccaggcgg aattcccaca gaccacggcc agcttctacg
    23941 ccagctttag cgaagtggcc agcttcaggc ttggtcacac ggttagcttt tttagcaccg
    24001 gtggtcacct gaatagcacg gtagccatcg ttagccaggt ctttaacctg agtaacgcgg
    24061 tttgcttcaa cttcgattac ggttactggg atagaaacgc cgtcttctgt gaagatacgg
    24121 gtcataccca cttttttacc gactaaacca atcattgttt caacctctca atcgctcaat
    24181 gacctgatta acccaggctg atctgcacgt ctacaccggc agccagatcc agacgcatca
    24241 gagcatcaac ggttttctcg gttggctcaa cgatgtcaac cagacgcaag tgagtacgga
    24301 tttcgtactg atcgcgcgcg tctttgttga cgtgcgggga gatcagaaca gtgaagcgct
    24361 ctttgcgtgt cggcagcggg atcggaccac ggacctgcgc accagtgcgc ttggcagtct
    24421 cgacgatttc cgcggttgct tgatcgatca gacgatgatc aaacgctttc aggcggatac
    24481 ggattctttg gttctgcatg aggccagagc tccaattatt ttataaacga aaatgattac
    24541 tcctcagacc cattacgatt gatgggagag tgtaaccgtt cttacgtagc tccccgattg
    24601 ggagcattgt taggtagcca aattcggcta actgaggttc agattgaacc tgctgtcaac
    24661 tacgacaagc ccgcgcatta tacatactta accaccgaac gcaagcgtat tgctagtaaa
    24721 gtaaacagcc ttcgggttat ctctcaagtt cgttcagtgc atttttgaca cgctctctta
    24781 aaccggcttt ctcttcgctt tcttccacag tattttcttc gtcttcagaa gaagaggggg
    24841 cattctcaac cgcgacgcct gggtcatctt cctcctcgac ggattcgacg acggggagtg
    24901 gctgtttttt gaagaaatag tgttgtgttg attgtttaaa agcgggcgtt tcagcctcta
    24961 cagtgacttc ggcttttact atccaaagca cccatagctg cctggcatac ccaccaacca
    25021 gcaccattcc tgcaaaccac aggcaaatca gtaataaaca aattacatat attatggtgc
    25081 tgtgtttttg tcgggagaag ataccagggt gacctgtttc tttctgctca cgcgcggcga
    25141 tatagaattc aaacatgtca tttccccttc actgttgttt ccggggaaat atttgcttca
    25201 tcccgatata agtaggcact ctcccctgcc acctgccaga gatgtactaa cgttgaaaag
    25261 ccaacaacac cgtcagtttt caagtggtgg cttttttgaa attgctttat tttttctacc
    25321 aacaagggac gccactccgc cgaaggttct gtcgaaatat gcaatgcgcg gtttaacatc
    25381 gtttccagcc agagaatctc ttcctcgctg ctatcacgcg taatggtact ctccccttcc
    25441 ggcgacattt tccacaacag aagataatca cccgtccata ccgactcaaa ccatttatgt
    25501 gtcagcgtcc acgtttgctg acctacgagg acatcaacgc tagcctcacc gacgcgaacg
    25561 accacaacag gcaatttttt gtccccgact ttaagcgatg cgatccaggg caaattctga
    25621 tctaccaatg tttgtaaaga agcattccca cttttacatg ccagccccgc tcttactgcc
    25681 tgatcgcacc aggccgaatc agctgggacc tcgtaccccc atacgccgta taacacgctt
    25741 agcgcctcac tatcgcgcat caccacatga gcaatatcct tagtcatatc ttgtttaact
    25801 actggcgtta cgggtatcag ccacgcagga acgggtaacg tagctgtaaa agaggacagc
    25861 agtagccaac cacaggtaag agcgataatg gttccactca tcaccacggc aagctgcatt
    25921 ttccgggggc ggttatcacc caatatgcgg taaatcattc gccattgttt tgctgtgaaa
    25981 acgctggtct gctcggcttg ctgtaataag cgaattgttt cgccggcaag cgccagcttt
    26041 ctgcgatcgc cccgacataa taaagctatc cgtttcagaa ctctggcggt taaaaggaga
    26101 tcgggttggt caatattctg accttcaagg atgcttgcct taagttcgtg ctccgtcaga
    26161 cgtcctatcg agacaagttc aatgccctgt tttttaaaat cacgttcgac ttttcgatcg
    26221 ggtctgccga tcagcaaaaa ttgaccgtca ggaataagag atattgcgcg ttggagatca
    26281 tcaagtatgc ggcggtgatt caggtgagca ttttcaataa taataagagg gcagcgacta
    26341 tcagttcgat gaagcgcatg cagtaattgc cccgccatct catctcgcgt ccaggcgctg
    26401 tcacggcagc cctgttcagc cgcgttatcg tctgtaacga tccatgcggc cttaccttgc
    26461 caggaaacaa cgaggcttaa agtaatcaca cggcgatgtt tatctttttg cagttgctgc
    26521 gccagccagg tttttccact tccagcctca cccaacaaat agcatagacg ccaggtttga
    26581 cgtttctcac acaaccagga aagaataact tctcttctcg tagacataga acttcctgtt
    26641 ttaattattg attatcctta atcataaaaa taatgtatgt atttattaat taaatacata
    26701 ttactaatat aaataaattt tgcttgattc atgcaagcgg cattaatact atttatacta
    26761 acgtcaatat acaacccacc caatctattt tattagaaca tacatcgtgc ccacactacg
    26821 tttccccttc catctggcga atcataataa agatgctgcc atcaatatat tgattatatt
    26881 tataagcata gggtcgatta tttttaatgt gaattatttc catacaacta tagttaaaaa
    26941 tggacagatc attaatcaac caacaaacgc atttcagtct gatttcagcc ttgccgcttt
    27001 atggcgtaac gaaaatcatg ctggggtaaa agatgcaaat cccgtggcag ttaatcagga
    27061 aacacctaag ctctctatag cgcttaatgg cattgtactc acatcgaatg atgagacttc
    27121 ttttgtctta attaatgagg ggagcgaaca aaagcgatac tccctcaacg aagcccttga
    27181 gtcagcgccg ggaacgttta ttagaaaaat aaataaaacc agcgttgtgt ttgaaactca
    27241 cgggcattac gaaaaagtaa ctctccatcc agggctacct gacatcatca agcagcctga
    27301 ttcagaaagt caaaatgtac tcgctgatta tattatcgcc acgcctatac gtgatggaga
    27361 acagatatac gggcttcgat tgaaccctcg taagggcctc aatgcattta ccaccagctt
    27421 gctacaacca ggggatatcg ccctcaggat caataatctt tcgctgaccc accctgatga
    27481 ggtttcacag gcattaagtt tactgctaac tcaacaaagt gctcagttta caattcgtcg
    27541 caacggcgta ccccgcttga taaatgtttc cgtcggggaa cttacaggaa tgaatggact
    27601 gcgtcatgaa aggactcaat aaaatcacct gctgcttgct ggcagcacta ctcatgcctt
    27661 gtgcaggaca cgctgagaac gaacaatacg gcgcgaactt caataacgcc gatatccgcc
    27721 agttcgtgga aatagtgggt cagcatcttg gcaaaacgat cctgatcgac ccttcggtac
    27781 agggaaccat ttccgtacgc agtaatgata cgtttagcca acaggagtac taccagttct
    27841 ttttaagtat tcttgatctt tacggttatt ccgtgatcac gctggacaat ggttttctga
    27901 aagtggttcg ctcagctaat gtaaaaacat cgccagggat gattgctgac agttctcgtc
    27961 caggcgtagg tgatgagttg gtcacccgaa tcgtaccgct tgagaacgtt cctgctcgtg
    28021 acctggcccc cctgctccgc cagatgatgg atgcgggtag cgtcggtaat gttgtgcatt
    28081 atgaaccctc caacgttctt attctgaccg gtcgtgcctc caccattaat aaactgattg
    28141 aagtcataaa gcgcgttgat gtcatcggca cagagaagca gcaaattatt catctggaat
    28201 atgcgtcagc ggaagatctc gccgagattc ttaatcaatt aatcagcgaa agccacggta
    28261 aaagccagat gccagccctc ctctccgcga agattgtggc ggataagcga accaactctc
    28321 ttatcatcag tggaccggaa aaagcacgcc agcgcatcac ttcattactg aaaagccttg
    28381 atgtcgaaga gagcgaggaa ggaaataccc gggtttatta cctgaaatat gctaaagcca
    28441 cgaatctggt ggaagtgcta accggtgttt ccgaaaagct gaaagatgaa aaagggaatg
    28501 cgcgtaagcc ctcctcttct ggcgcgatgg ataacgtcgc cattaccgcc gatgaacaga
    28561 ctaactctct ggtcattacc gctgaccagt ccgcccagga aaaactcgcc acggtaattg
    28621 cgcgtctgga cattcgccgt gcacaggtgc tggttgaggc aatcatcgtt gaagttcagg
    28681 atggaaatgg actaaacctc ggcgtgcaat gggcgaataa aaacgttggc gcacagcaat
    28741 ttaccaatac cggattaccg atttttaacg ctgcgcaagg tgtggctgat tataaaaaga
    28801 atggtgggat caccagcgcg aatcctgcct gggatatgtt tagcgcctac aatggcatgg
    28861 ccgcaggctt cttcaatggc gactggggag tactgcttac cgcgctggcc agtaacaata
    28921 aaaatgacat cctcgccacc ccaagcatcg taacgctgga taataaactc gcgtccttca
    28981 acgtggggca ggatgtgccg gtgctatccg ggtcacagac cacttcaggg gataacgtct
    29041 ttaataccgt cgaacgcaaa acggtgggga caaaactcaa agttactccg caggtcaatg
    29101 aaggcgacgc ggtgttgctc gaaatagagc aggaagtctc cagcgttgac tcttcctcta
    29161 actcgacgct cggcccgacg tttaataccc gtactattca aaacgccgtg ctggtcaaaa
    29221 ccggtgaaac ggtggtcctg ggcggattgc tggatgattt ttctaaagag caagtgtcaa
    29281 aggttcctct gcttggcgat attcctttag tggggcaact cttccgctat acctccaccg
    29341 agcgcgctaa acgcaacctg atggtattta tccgtccgac gattatccgt gacgatgatg
    29401 tttatcactc actgtcaaaa gagaaataca cccgttaccg tcaggagcaa caacagcgga
    29461 tcgacgggaa atcaaaagcg ctggttggct cggaagattt gccggtgctg gatgaaaaca
    29521 cgttcaacag tcacgcccct gcgccatcgt cacggtgagg cattcatatg agaattcact
    29581 caccgtaccc cgccagttgg gcgctggcac aacgaattgg ttatctctat tcagagggcg
    29641 agattattta tctcgccgat acgccattcg agcggttact cgatattcaa cgtcaggttg
    29701 gccagtgcca gaccatgacc agcttgtcac aggctgattt tgaagctcgg ctggaagcgg
    29761 tattccatca gaataccggt gagtcgcaac agattgcgca ggatatcgat caatccgtcg
    29821 atcttctctc gctttcggaa gagatgcccg caaataaaga tctcctgaat gaagattcag
    29881 cggcaccggt tatccgcttg atcaatgcga ttttgagtga ggccatcaaa gaaaccgcct
    29941 ctgatatcca cattgaaacc tatgaaaaaa caatgtcgat ccgttttcgc atcgacggcg
    30001 ttttgcggac aattttacag ccaaacaaaa aactggcggc actgcttatc tcccgaatta
    30061 aggtcatggc tcgtcttgat atcgccgaaa aacgtattcc acaggatgga agaattagtt
    30121 tgcgtatcgg gcgacgtaac atagatgtcc gcgtatccac actgccgtcc atctatggtg
    30181 aacgcgccgt actccgcctg ctggataaaa acagcctcca gctttcattg aacaacctgg
    30241 ggatgacggc agcggataag caggatttag aaaatctcat tcagcttccg cacggtatta
    30301 tcttggtgac agggccgaca ggctccggta aaagcaccac gctctacgcc atcctttcgg
    30361 cgctgaatac tcccggccgc aatattctga cggtagaaga tcccgtggaa tatgagctgg
    30421 aaggcattgg gcaaacgcag gtgaataccc gtgtggatat gtctttcgct cgcggcctgc
    30481 gcgccatact tcgccaggac ccggatgtcg tcatggtggg ggaaattcgt gatacagaaa
    30541 ccgcgcagat tgcggttcag gcctcgctca ccggccatct ggtactctca acactccaca
    30601 ctaacagtgc atcaggcgca gtgacccggc tccgcgacat gggcgtcgaa tcattcctgc
    30661 tttcgtcttc cctggcaggg attatcgcgc aacgtctggt tcgtcgcctg tgtccgcaat
    30721 gccgacaatt cacgcccgta tcaccccaac aagcgcagat gtttaaatat catcagctcg
    30781 cggtgacaac aattggcact cccgtaggct gccctcattg ccatcaatcc ggctatcagg
    30841 ggcgcatggc gatccacgaa atgatggtgg tgacgccgga attacgggcc gctattcatg
    30901 aaaatgtgga tgaacaagca ctggagcgac tagtccggca acaacacaat gccttaatca
    30961 aaaatggcct gcaaaaagtg ataagcggtg acacctcctg ggatgaggtt atgcgcgtcg
    31021 ccagtgccac gctggagagc gaagcatgaa ttatcgctat cgcgccatga cccaggatgg
    31081 tcaaaaattg caagggatca ttgatgctaa cgatgaacgt caggcacgac tgcggctgcg
    31141 tgaagaaggg cttttcctgc tggatattcg cccccaaaaa agttcgggag taaaaacacg
    31201 tcgcccgagg atcagccata gtgaactgac gcttttcacc cggcagttgg caaccttaag
    31261 cgcagcggca ttacccctgg aagagagcct tgccgtaatc ggtcaacaaa gcagtaataa
    31321 acgactgggt gacgtgttaa atcaggtacg cagcgccatc cttgaagggc atcccctttc
    31381 cgatgcatta cagcattttc ccacgctttt cgattcgctc tatcgtaccc tggtaaaagc
    31441 gggcgaaaag agcgggctgc tggccccggt gttggaaaag ctggctgatt acaatgaaaa
    31501 ccggcagaaa atccgcagca agctcattca gtcactgatc tacccctgta tgctcactac
    31561 ggtggcgatt ggggtcgtga ttattctcct cactgctgtc gtgcccaaaa ttaccgaaca
    31621 gttcgtgcat atgaagcagc aactgccgct gagtacacgc attcttttag gtctgagcga
    31681 cacgttgcaa cgtaccggcc cgacattatt agcgacagtg tttattgtcg ctgtaggttt
    31741 ctggctctgg ttaaaacgcg gcaataaccg ccaccgtttt catgccatgt tgctgcgcgt
    31801 tgcgctcatc ggcccgctga tttgcgccat taacagcgca cgctatctcc gcactttaag
    31861 tattttgcaa tccagcggcg tccctctgct ggatgggatg aatttgtcca ccgaaagcct
    31921 caacaacctt gaaattcgcc agcgtctggc aaatgcggca gagaacgttc gccagggtaa
    31981 cagcattcat ctttcgctgg aacaaaccgc aattttcccg ccgatgatgc tctacatggt
    32041 ggcctctggc gaaaaaagcg ggcagctcgg cacattaatg gtcagagccg cagataacca
    32101 ggagacactc caacaaaatc ggatcgcctt aacgctctcc atcttcgagc cagcactcat
    32161 tattacgatg gcactgatcg tcctgtttat tgtcgtgtcg gtactccaac ctcttcttca
    32221 acttaactca atgattaatt aaggaaagca tatgcgcgca acggataagc aacgcggttt
    32281 tacattactg gaaattatgg tggtgatcgt cattatcggc gtacttgcca gcctggtggt
    32341 tcctaaccta atgggcaata aagaaaaggc ggataagcaa aaagtcgtca gcgatattgt
    32401 ggcgctggaa aacgcccttg atatgtacaa actcgacaat catcactatc caaccacaaa
    32461 tcaggggctt gaatccttag tagaagcgcc gacactgcca ccgctggccg caaactataa
    32521 caaggaaggt tatatcaagc gtctgcctgc cgatccctgg ggcaatgatt atgtcctcgt
    32581 taatcctggt gaacatggtg catacgatct gctttcagca gggcccgatg gtgaaatggg
    32641 aaccgaggac gacatcacca actggggttt gagcaagaag aaaaagtaag tcagcgatga
    32701 atcagcaacg cgggtttacc ctgctggaga tgatgctggt gctggcgctg gtcgctatca
    32761 cggcaagcgt ggtgctcttc acatatggcc gggaggatgt cgccaacact cgtgcgcgcg
    32821 aaacagcggc gcggtttacc gctgcgcttg aattagccat cgaccgcgcc acgctcagtg
    32881 gtcagcctgt cggtatccat ttctctgatt cagcatggcg cattatggta ccgggcaaaa
    32941 caccatcagc ctggcgctgg gttccattac aagaagatgc cgcagacgag agccaaaatg
    33001 actgggatga agaactctca attcatcttc agccatttaa acctgatgac agcaaccagc
    33061 cacaggttgt gattctggcc gacggacaaa tcacgccctt ctctttgttg atggctaacg
    33121 ccgggaccgg tgagccgctc ctgacgttag tctgttcagg ctcatggccc ctggatcaaa
    33181 cgctcgccag agatacccgc ccatgaacaa acaatcaggg atgacactgc ttgaagtctt
    33241 actggcgatg agtattttca ccgctgttgc tctgacttta atgagcagta tgcaagggca
    33301 acgaaatgcc atcgagcgaa tgcgtaacga gacgctggca ttgtggatag cggataacca
    33361 gcttcagtca caagattcat tcggcgagga aaacacgtcc agttctggca aagagttaat
    33421 aaatggcgaa gagtggaact ggcgcagcga tatccactca agcaaagacg gcacgcttct
    33481 ggaacgcacc ataacggtga cattacccag cggtcagaca acctcgctta cgcgttatca
    33541 aagcatcgat aataaatccg ggcaggcaca agatgattaa tcgccagcag ggtttcacat
    33601 tgctggaggt catggcggcg ctggcgattt tctcaatgct tagcgtgctg gcgtttatga
    33661 ttttttcgca agcttccgag ttgcatcaac gctcacagaa agaaatccag cagttcaatc
    33721 agctacagcg caccatcacc attcttgata acgatctcct gcaactggtc gcccggagga
    33781 atcggagtac ggacaaaatc atggtgttgg gtgaagaagc tatttttacc acgcaaagtc
    33841 gcgatccgct ggctcctctc agtgaagcac aaaccttact cactgttcac tggtatctgc
    33901 gaaatcatac gctctaccgt gctgttcgta cttctgtgga tggcaggaag gatcaacccg
    33961 cccaggcgat gctggaacat gtcgagagct ttcttctgga aagtaacagc ggggaaagcc
    34021 aggaacttcc tctctcagtc acgttacatc tgcagactca gcaatatggg gggttacagc
    34081 gccgttttgc attacctgag cagttagcga gagaagagtc gccagctcag acgcaggccg
    34141 ggaataataa ccatgaataa tgaacaacgc ggcgtcgcgc tgctcattgt actcatgtta
    34201 ctggcgctca tggctgctct ggcagcagac atgacgctca gttttcacag ccagcttcag
    34261 cgaacccgcc aggtaaaaca tcatctgcaa cggcaatatg atattgaact ggcggagaaa
    34321 cttgcgcttg ccagcctcac ccaggacgtt aaagataacg acagacagac cacgttgcag
    34381 cagtactggg cacagccaca acagttgcag ctggaagatg gcaatacagt gaagtggcag
    34441 ttacgcgatg cccagcactg ttttaatctc aatgcgttag caaaaatctc tgacgacccg
    34501 ctggcttccc ctgatttccc cgcccaggta ttcagtgcgc tgctgattaa cgccggtatc
    34561 gatcgcggaa acaccgatga gatcgtgcaa tcaatcgctg actatattga tgtcgatgac
    34621 tcaccacgct ttcacggcgc agaagatagc ttttaccaaa gccagacgcc gccccggcat
    34681 agtgccaatc agatgctttt tctgactggc gaattacgtc agataaaagg cataaccgaa
    34741 aacatctatc agcggcttat cccgtatgtc tgcgttctcc cgaccacgga acttagcatc
    34801 aatcttaata tgctaacgga gaacgatatt ccgctcttca gagcgttatt cctcaataac
    34861 atcacggatg ctgacgctcg ggtcttattg caaaaaaggc cgagggaagg ctggctaacc
    34921 acggatgcat ttctttactg ggcgcagcag gatttctccg gtgtgaagcc actggttgct
    34981 caggtgaaaa ggcatctctt tccctatagc cgctatttta cgctgagtac ggaaagtatc
    35041 agcgacgaac aatcccaggg atggcaaagc catatttttt tcaaccgtaa acagcagagc
    35101 gcccaaatct atcggcgtac gctgcaactc tactaatcat aaggatcgtg atgcctgaat
    35161 cgttgatggt catacgttct tcctcgacgc tgcgtaagca ttgggaatgg atgacgttct
    35221 ccgcagatag cgtatcttcg gtacatacgc tcacggacga tcttccgctg gagtcgctgg
    35281 ccgatcagcc cggtgccgga aatgttcatc ttttgatccc accagagggc cttttatatc
    35341 gttccctcac gctgcccaac gccaaataca aactcaccgc acaaacatta caatggctgg
    35401 cagaggagac gttacccgac aacacccagg actggcactg gacggttgtc gataaacaaa
    35461 acgaaagtgt cgaggtgata ggcattcagt cggagaaact cagtcgctat cttgagcgtt
    35521 tacatactgc cggactcaac gtgacgcggg ttttgccaga tggctgctat cttccctggg
    35581 aagtagatag ctggactctg gttaaccagc aaacaagctg gctcattcgc agtgccgcac
    35641 atgccttcaa cgaactggat gaacactggt tacagcatct tgctgcccag tttccgcctg
    35701 agaatatgct ttgttatggt gtagtacctc acggcgttgc agcggccaat ccacttatcc
    35761 agcatcctga aataccatca ctgagtttgt attctgccga tattgcattc cagcgctatg
    35821 acatgctgca tggtattttt cgtaaacaga aaactgtaag caaatcggga aaatggctcg
    35881 cccgcctggc agtaagctgt ctcgttctcg ccattctttc ttttgttggc agccggagca
    35941 tcgcgctctg gcatacgctt aagattgaag atcaacttca gcaacaacag caagaaacct
    36001 ggcaacgtta tttcccgcag atcaaacgca cccacaactt tcatttttac tttaagcagc
    36061 aactcgctca gcaatatcct gaagctgtac cgttgctcta tcatttacaa acacttctgc
    36121 tggaacaccc tgaactgcag ttaatggagg cgaactatag ccagaagcaa aagtcgctga
    36181 ccttaaaaat gagtgctaag agtgaagcca atatcgatcg tttctgtgag ttaacacagt
    36241 cctggcttcc gatggaaaaa acagaaaaag atccggtcag tggtgtatgg acagtaagga
    36301 actcagggaa atgattaaat catggtgggc agaaaaatcc acatcggaaa aaaagatcgt
    36361 tgcggcactg gcggttctct cgctcggcgt tttttgttgg cttggggtga taaaaccgat
    36421 agatacctac attgcagagc atcagtccca cgcgcaaaaa attaagaagg acatcaaatg
    36481 gatgcaggag caagcgagca cccatggttt actcggtcat cctgcgctta cgcagcccat
    36541 caaaaatatc cttctcgagg aagcaaaacg tgagaatttg gctatcacgc tggaaaacgg
    36601 cccggacaat actctgacaa ttcatcctgt cacagctccc ctcgaaaacg tctcccgctg
    36661 gctcactacg gcacaggtaa catacggtat tgttattgaa gaccttcagt ttactcttgc
    36721 cggaaacgaa gagatcaccc tcaggcattt gtccttcagg gagcaacaat aatgacaatg
    36781 ctactgccgc tcttcattct ggttggtttt attgcaggtt attttgttaa tgccatcgcc
    36841 tatcacctct cgccgttgga ggataaaacg gcgttaacgt ttcgccaggt attggttcat
    36901 ttcaggcaaa aaaaatatgc ctggcatgat acagtgcccc tgatactttg tgttgctgct
    36961 gctatcgcct gcgccctggc acctttcacg cccatcgtga ctggcgcact ctttctctat
    37021 ttctgtttcg tactcacgct cagtgttatt gattttcgca ctcagctcct gcccgacaaa
    37081 ctcaccttac cgctgctctg gcttggcttg gtatttaatg cgcagtatgg attaattgat
    37141 ttacatgatg cggtttacgg cgcggtagcg ggctatgggg tgctgtggtg tgtttactgg
    37201 ggcgtctggt tagtttgtca caaagaggga ttgggctacg gtgatttcaa gctactggcc
    37261 gctgcaggcg catggtgtgg ctggcaaacg ttgccaatga tactgctgat tgcctcgctg
    37321 ggtggcattg gttacgccat cgtttcacaa cttctgcaac gccggactat aaccactatc
    37381 gcattcggcc cgtggcttgc gctcggcagc atgataaacc tggggtatct ggcctggatc
    37441 tcttattaac cgggagggtt ctccctcccg acacggctca accttcttcg cggatctgtg
    37501 cttgcagata attttgcagg cccatcttct gaatcagatc aagttccgtt tccagccagt
    37561 cgatatggcc ttcttcatca cgcaaaattt ctatcatcat atcgcggctg acgtaatcat
    37621 gaacgctatc ggcataacca attgcctcac gcaaattctt cgcgccatcc agctcaagtg
    37681 ccagatcaga acgcagcatt tcctcaacat cttcaccaat gttcagtttg cccaggtcct
    37741 gtaagtttgg aagaccttcc agaaaaagaa tgcgctcaat ataacgatcg gcgtgtttca
    37801 tctcatcaat ggattcatga tactccacat cattgagacg tttgagaccc cagtttttaa
    37861 acattcgggc atggagaaag tactgattga ttgcgacaag ctcatttccc aacagtttgt
    37921 tgagataatt tataacttta gtatcacctt tcattttttg actccttcgc ttccactctt
    37981 tgaagagtag aacggcttac gagtaagtca aaaaacacac tccgtttatg cggactcctt
    38041 aaactccggc agctgcatta attcatcctc catcacttca cgcgcggccc ggacacattt
    38101 accgcactga ttgccgaccg gaataaattt ttttaattgc tggaacgagt gaggggaaaa
    38161 ctggcgcaca gcctgacgaa tttttttatc gctgatacca ttacaaagac aaacgtacat
    38221 agagactccc gtatactttc ttcaaaagta aatgaaaata gttcttattt caattactga
    38281 aagactcttt tttaagcaaa gaatagaaat atgaataaaa ataatgggct accaaaagta
    38341 gcccattgac aaaaaatgcg gcgatactgg aaggtatcgc caacacattt attgcttagt
    38401 aaacggcgcg aagatttcgc taaatgcgta cttcggtgcc tgttctttag tcaggccgtg
    38461 gaattccgga gacaggttag tgccgcccgg caggtcgcgc gcgattgacc agataccaac
    38521 cataccgaga ttacgcttct gcgcatcctg catgaccaga cgagcatcag agagatagaa
    38581 cacctcgccc tgaacgtcgt tcacgccaac catcggcgtg gtacccatca tagcgtcaat
    38641 ttcagcatcg ctcttattgg gatggaggcc tttcaattgt gaatgcaggt tggcaatcgc
    38701 agacgttgca cacttaccgt gaatgttctg gccttcggta tttgcagact gacaaatcgc
    38761 gttaccgtag tccattgtca tcacgttcac acccgccagc tcaacacctt tcgctttggc
    38821 atcgctcagg acattcatcc cttccggcgt caggccagtc ggcagaattg gcaaggtgta
    38881 ccagatagca atatctttgc cttctgactt ccatttatcc tgcactttct tcacagcaag
    38941 gttacgacgt tcaatagatg cctgatccgc aacccaggtg ccttcgatat cgaagtccag
    39001 gactttgagg ttcaggttat caacgatgtc ataataatgc tgcatcagat cgtctacgtt
    39061 cttacaggaa gcagccagcg gagcgttgtt agcaccaccg atagacagca tcacatcgcc
    39121 gccagcctca cgcagagctt tgattttgct gtactgagcg taattctgca taccgtaagc
    39181 ggtaccccat gtcggcagac aggtgttcgc atctttactc accacaaacg ccagcgtgaa
    39241 gtggttgacg ttatgattct tagccagcgc agccagatcc gggatcgtat tcagcgtaaa
    39301 gtcgacatac ggtgcataaa cgtgtttcgg ccacgctttc ttcggcgtac ctacgcgctc
    39361 tttggttccg gtccagtcaa caaaaacacg ccacgggttg ctgtcagaag gagaaacttt
    39421 ctgcacttta ctctgagaaa tgtagttcac accgttaaag cgaatcagcg tatcgctggc
    39481 ataaagcgtt tctggattaa actgcggcgc attattcagc tcttcgttac tatagctctg
    39541 agccttacct aacggcttcc acgggcggct attgctaccg gtggcgtttt ggttcgccac
    39601 cagagcaggg ttgtcacttt gcgtccagaa cagcgcttca taagcctgcc catcaacaac
    39661 aacgcgatca cctttcacgt acacagtgga tgcggaccat gctttcgccg gttcgtattt
    39721 cttccacggg ttatcgccgc ccgggacaaa gccccatgcg tcttgatcga cagaagtttt
    39781 ataatgtgca tcgttataat ctacgatgct gtccgcgctg tacgctttgc tggcctggaa
    39841 accatccgca acaatgacgc cgccgttatc aatttcacag gtagtcgggt taccgtactg
    39901 actcaattcc gcagcggtag ctgtacgctc gagacgccat gggttattgg agttttcagc
    39961 gctggtaccc cgtgggcaat ctttagaccc aacccaccag gcgttcttgt aaacattacc
    40021 attgaaaata acatggtaaa tttcgctacc ttcctggccg ctccacgctt gcaatgagta
    40081 atcgttaacg ctatccgctg gtgttgatgg ctcgctgtta tccggagttg gggtcaccgg
    40141 tgtttccgga gttggatcaa ccggagtcac agcaccgcca ttattatccg gcttaacgga
    40201 acaggagcca gggttaccat actggctgat ttctgttgcc gttgccgcgc gaacataacg
    40261 ccatgggttt ctggcatcgt tttctttcgc atcgcctggg caatttgccg cgcctaccca
    40321 ccaggcgttc ttataaatgc cgccgttata gataacgtac catgtagaag aaccttcctg
    40381 acctttccag gctacgacag catagttgtc gtttgactta tcagctgggg ctggctgaac
    40441 atcaggagcc ggtttaacgt ccggtgccgg tttcacatcc ggtgcaggct gtggtgctga
    40501 agtacaggac tgtggattac tggtttctga gatttccgtt gccgtagcgg cacgaacata
    40561 acgccatggg ttgctggcat cattgccttt cgcatcaccc gggcagttag aagaggctac
    40621 ccaccaggca ttcttatata ccgcaccatt aaagacgaca taccaggtct gaccgccctg
    40681 ctgtttattc caggcaacta cagaagaatt agacggaaca gtgccctgtg ctggtgtagc
    40741 cgaaccgcca ttagccggcg tattggatgc aggcgtattt gaatttgaag aagatgagct
    40801 gccgctcttt tcgcaggaaa gtgtattgcc atactgacta atttcagcag ctgtcgcggt
    40861 acgctttaaa cgccacgggt tagttgcatc atttactttc gcttttcccg ggcaatttgt
    40921 agaagaaacc caccaggcat tttcataaat tttgccatcg aaaataacct gatatttatt
    40981 accaccttgt tggttattcc atgcttccat tgccaatgct ggcagagcgg aacacaccag
    41041 ccccatacca atcatagatt tagtaaatat att
//
LOCUS       DAOFQD010000027        39755 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.027, whole genome shotgun sequence.
ACCESSION   DAOFQD010000027 DAOFQD010000000
VERSION     DAOFQD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.027"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(13..870)
                     /gene="murI"
                     /locus_tag="QBF46_001541"
     CDS             complement(13..870)
                     /gene="murI"
                     /locus_tag="QBF46_001541"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDQ6673736.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPGAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(815..2659)
                     /gene="btuB"
                     /locus_tag="QBF46_001542"
     CDS             complement(815..2659)
                     /gene="btuB"
                     /locus_tag="QBF46_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDQ6673737.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2724..2914)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3028..4128
                     /gene="trmA"
                     /locus_tag="QBF46_001543"
     CDS             3028..4128
                     /gene="trmA"
                     /locus_tag="QBF46_001543"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDQ6673738.1"
                     /translation="MTPEHLPTEQYEAQLAKKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAESLRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4168..4527)
                     /locus_tag="QBF46_001544"
     CDS             complement(4168..4527)
                     /locus_tag="QBF46_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDQ6673739.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4527..5177)
                     /gene="fabR"
                     /locus_tag="QBF46_001545"
     CDS             complement(4527..5177)
                     /gene="fabR"
                     /locus_tag="QBF46_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDQ6673740.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5508..6908
                     /gene="sthA"
                     /locus_tag="QBF46_001546"
     CDS             5508..6908
                     /gene="sthA"
                     /locus_tag="QBF46_001546"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDQ6673741.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6891..7808)
                     /gene="oxyR"
                     /locus_tag="QBF46_001547"
     CDS             complement(6891..7808)
                     /gene="oxyR"
                     /locus_tag="QBF46_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDQ6673742.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8075..9448)
                     /gene="argH"
                     /locus_tag="QBF46_001548"
     CDS             complement(8075..9448)
                     /gene="argH"
                     /locus_tag="QBF46_001548"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDQ6673743.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9509..10285)
                     /gene="argB"
                     /locus_tag="QBF46_001549"
     CDS             complement(9509..10285)
                     /gene="argB"
                     /locus_tag="QBF46_001549"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDQ6673744.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10293..11297)
                     /gene="argC"
                     /locus_tag="QBF46_001550"
     CDS             complement(10293..11297)
                     /gene="argC"
                     /locus_tag="QBF46_001550"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDQ6673745.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11451..12602
                     /gene="argE"
                     /locus_tag="QBF46_001551"
     CDS             11451..12602
                     /gene="argE"
                     /locus_tag="QBF46_001551"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDQ6673746.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            12954..15605
                     /gene="ppc"
                     /locus_tag="QBF46_001552"
     CDS             12954..15605
                     /gene="ppc"
                     /locus_tag="QBF46_001552"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDQ6673747.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15788..17521
                     /locus_tag="QBF46_001553"
     CDS             15788..17521
                     /locus_tag="QBF46_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDQ6673748.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17736..18587
                     /locus_tag="QBF46_001554"
     CDS             17736..18587
                     /locus_tag="QBF46_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6673749.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18574..18915)
                     /locus_tag="QBF46_001555"
     CDS             complement(18574..18915)
                     /locus_tag="QBF46_001555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDQ6673750.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18917..19795)
                     /locus_tag="QBF46_001556"
     CDS             complement(18917..19795)
                     /locus_tag="QBF46_001556"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDQ6673751.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19761..22058)
                     /locus_tag="QBF46_001557"
     CDS             complement(19761..22058)
                     /locus_tag="QBF46_001557"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDQ6673752.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22109..22429)
                     /locus_tag="QBF46_001558"
     CDS             complement(22109..22429)
                     /locus_tag="QBF46_001558"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDQ6673753.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22444..23523)
                     /locus_tag="QBF46_001559"
     CDS             complement(22444..23523)
                     /locus_tag="QBF46_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDQ6673754.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23832..26333
                     /gene="ptsP"
                     /locus_tag="QBF46_001560"
     CDS             23832..26333
                     /gene="ptsP"
                     /locus_tag="QBF46_001560"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDQ6673755.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKSVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26345..27007
                     /gene="fsa"
                     /locus_tag="QBF46_001561"
     CDS             26345..27007
                     /gene="fsa"
                     /locus_tag="QBF46_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDQ6673756.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKLLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27018..28121
                     /gene="gldA"
                     /locus_tag="QBF46_001562"
     CDS             27018..28121
                     /gene="gldA"
                     /locus_tag="QBF46_001562"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDQ6673757.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28396..29013
                     /locus_tag="QBF46_001563"
     CDS             28396..29013
                     /locus_tag="QBF46_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDQ6673758.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGLARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29040..29945)
                     /gene="yijE"
                     /locus_tag="QBF46_001564"
     CDS             complement(29040..29945)
                     /gene="yijE"
                     /locus_tag="QBF46_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDQ6673759.1"
                     /translation="MSASGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30038..32218)
                     /gene="katG"
                     /locus_tag="QBF46_001565"
     CDS             complement(30038..32218)
                     /gene="katG"
                     /locus_tag="QBF46_001565"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDQ6673760.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKFTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32547..33437)
                     /gene="metF"
                     /locus_tag="QBF46_001566"
     CDS             complement(32547..33437)
                     /gene="metF"
                     /locus_tag="QBF46_001566"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDQ6673761.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33786..36218)
                     /gene="metL"
                     /locus_tag="QBF46_001567"
     CDS             complement(33786..36218)
                     /gene="metL"
                     /locus_tag="QBF46_001567"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDQ6673762.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36221..37381)
                     /gene="metB"
                     /locus_tag="QBF46_001568"
     CDS             complement(36221..37381)
                     /gene="metB"
                     /locus_tag="QBF46_001568"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDQ6673763.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQVLRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37658..37975
                     /gene="metJ"
                     /locus_tag="QBF46_001569"
     CDS             37658..37975
                     /gene="metJ"
                     /locus_tag="QBF46_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDQ6673764.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38159..38767
                     /locus_tag="QBF46_001570"
     CDS             38159..38767
                     /locus_tag="QBF46_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDQ6673765.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            38912..39587
                     /locus_tag="QBF46_001571"
                     /pseudo
     CDS             38912..39587
                     /locus_tag="QBF46_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         9722 a        11004 c         9991 g         9038 t
ORIGIN      
        1 cattcaacca aatcagccta aaactgccag tttttcgagc gtttcgaagc cgtaacgctg
       61 taaaacgggc aataattgtt cagctcctgg cgtcattgcc atacaaaagg caatattcgc
      121 atcggcagat tttgcatccg gggcttcatg ttctaacaac caggccgttc ggcgagcaat
      181 cgctgcgcca gaatccacca gccgggttcc ctctggcagc acttgtaaca gttcttcttg
      241 tagtagaggg aaatgggtgc aacccaatac aacggtatct ggcggctctt tcattcttaa
      301 ccacgggcgt aggatacgtt ttagtgcatc cagagaaaca tcttcgccat gtagcttcgc
      361 ttcagccaac tcaaccatct ctgccgagcc cagcatttct atctggcatt cattagcgaa
      421 acgcgcgatc agctcatgag tataagaacg tttaactgtt ccgcgggttg ccagtaatcc
      481 gacaatgcca tttgccgtca gacgtgcagc aggtttaatc gccggcacga caccaacaac
      541 cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg gtactggcag tgttgcaagc
      601 gaccacagcc agcgcaaggg gataacgttc ttgcaccgcg gtgacaattg ccaccactcg
      661 ctcaacaata aacgcttcgc ttttttcgcc atacgggaaa gcgacgttat cgaaagcata
      721 aatgtaatgg agatccggta agagatgccg gatctcgtca tagaccgaca acccaccgac
      781 gccggagtca aacaccagca cggtgggacg tggttcagaa ggtgtagctg ccagacaagg
      841 tgtattcccg tcctgcagtt tggtagccat agactgtctc ataatctttg tcgaacaggt
      901 tggctatttt accacgaact gtcaggtgag aggtgaccgg atacgcaacc gcaagatccc
      961 acaagctcac accgcccatt ttaacggttt gataaggata agatgagtaa tccttatcat
     1021 agcgagtgcc taaatactga taagtaatac cccagtcgaa gtcatacaac tgccagtcga
     1081 gctggtattt cacctgctgt ttagcacggc gtaacaacgg cgtgtcggta atcgcattgc
     1141 gcgcatcgac ataatcataa ctcacagtat gcgtcagtgg tccggtatca aaattggcgg
     1201 tcgcctcgac gcccttaatc cgcgctttcc cttcgttgta atatttcagg gtgtgatcat
     1261 cataatcgat caagtcactg acatcgttac gatatccgga aatacgccag ttcaccccag
     1321 cggttaagcc ttcaaacgcg ccttcccact gtttgctttt ctccgggtcc agattcggat
     1381 ttccgtagaa gccatacagt tgccccagat ttggtgcctt ataagatgtc ccgtaggaag
     1441 caatgaagcg ataaccttcg atgaattccc aaccggcgct ggtttgccag gttccatgac
     1501 gaccaaactg tgagttatcg tcactgcgtg ctgcgccttc aaaggtaaaa tcgccgactt
     1561 gttgcagccc ggtcagatag atgccggtat tacgttgatc atatccatcc tcaacataac
     1621 ctgtacccgg cgtcgtagtc tgtttctgcc agtcgacacc cgcaccaata ctaccgtgac
     1681 caacgatgac attgtttgcc cactggacgg tgtattgctt catctcatcg agcgtcgccg
     1741 acgaatcata acgaccataa tggggatcgt agttgtaatc tttgctatgg ctatagctgg
     1801 taatgagttg tgatttaatc agttcgccgt tatagcgcag cccggcgtcc caactttggc
     1861 tatagagttt acgggtatcg agcaacggtg aaccgggaga ataatacgca tcataattgg
     1921 tacggttatc atagccatag ccgcgcacaa agccgctcca ggcatcagta aagttatgct
     1981 ccagcgcgcc ataaagcgtt ttacttaaaa aaccatcgtt atctgtctgc gcttgcgttc
     2041 cggtattacc ataggcaaca acatcataac catgagtatg ggcataatcg cccaacagcg
     2101 ttacccgtgt cttatccccc agttgttgct gcgtagagac atcatagttc tgataactat
     2161 tgcttcccca ccctgctgaa atttccgttc cgggttcatc gcgcgtcgtg atgatattca
     2221 ccaccccgcc tattgcatcg gaaccataaa cagcggagcg cggcccacgg atatattcaa
     2281 cacgctggac aagcgcaata gggaactggc taaggtcggc agaaccactc acccccgcca
     2341 gattcaggcg tacgccatca attaacacca acacatgact ggcatttgta ccgcgaataa
     2401 aaatagatga gagctgacct gaaccgccgt tttgggtgat atcgacgccc ggaagacggc
     2461 gcagcacatc attgaccgag gtcgactgcc agcggtcgat atcctgacgg gtcacaacgg
     2521 tggttggtgc aagcacagtg ctgcgcggct gttcaaaacg gttagcagta acgacgagag
     2581 tatccgggct ggtatcctgt gcccaagcgg aaaaggctgt gacagaacac gccgtcagca
     2641 gcgaagcttt tttaatcatt gtaaagcatc cacaatagaa gaaggatgcc gcaggtttca
     2701 tcaatattac gcgatgatga gaaccagatg cgacgttggc cggcaggtct tcgggcttgg
     2761 aggggtatct aagatactaa gagatgatga cttcccaccg gatggcagtg tccgcataac
     2821 gcaatcatcg cacctttcct taccgctgcg cgtcagctcc agattcgcac tggattccct
     2881 attaactcac aggaccggca agtggatgct acaggttgta acaagttact gtccagacgt
     2941 agctcacaaa taggaattca tcaagatctg gacatctgat gagcaatccc tacaatcgcc
     3001 gcgtacttta atttttcagg atacatcatg acccccgaac accttccaac agaacagtat
     3061 gaagcgcagt tagccaaaaa agtggtacgt ttgcaaagta tgatggcacc gttttctgac
     3121 ctggttccgg aagtgtttcg ctcgccggtc agtcattacc ggatgcgtgc ggagttccgc
     3181 atctggcacg atggcgatga cctgtatcac atcattttcg atcaacaaac caaaagccgt
     3241 atccgcgtgg atagcttccc cgccgccagt gaacttatca accagttgat gacggcgatg
     3301 attgcgggtg tgcgtaataa tcccgttctg cgccacaagt tgttccagat tgattacctc
     3361 accacgctga gtaatcaggc ggtggtttcc ctgctgtacc ataaaaagct ggatgatgag
     3421 tggcgtcagg aagcggaatc cctgcgcgat gcactgcgag cacagaatct gaatgtgcat
     3481 ctgattggtc gggcaacgaa aaccaaaatc gagctggatc aagattacat cgatgaacgt
     3541 ctgccggtcg cagggaaaga gatgatctac cgtcaggtag aaaacagttt tacccagccg
     3601 aacgcggcga tgaatattca gatgctggaa tgggcgctgg acgtaaccaa aggctcaaaa
     3661 ggcgatttac tggagctgta ctgcggcaac ggtaactttt cattagcgct ggcgcgcaat
     3721 tttgatcggg tattagccac cgaaatcgct aagccgtcgg ttgctgctgc gcaatacaac
     3781 atcgcagcta accatattga taacgtacaa attattcgta tggcggcaga agaatttact
     3841 caggcgatga atggtgtgcg cgagtttaac cgcctgcaag ggatcgactt aaagagttat
     3901 cagtgcgaaa ccatttttgt cgacccaccg cgcagcggtc tggacagtga aaccgagaaa
     3961 atggtgcagg cgtatccgcg tattttgtac atctcctgta acccggaaac gttatgcaag
     4021 aatctggaaa cattaagcca gacgcacaag gtcgaacgtc tggctctgtt tgatcagttc
     4081 ccctacacgc accatatgga gtgcggcgta ttactgaccg cgaagtaaaa cccatgccgg
     4141 atgcgccagc atccggcata ataccgatta ctcagcaact tcctgcttac ggttacgcat
     4201 cttcgcgcca atccagaaca ccataatgac ggagagtact gccgggaaga agttagagcc
     4261 gatatccgga tattccgcac gaactaccgt gctgtacagc agtacgccga gaataaaaca
     4321 ggcagctgcc agacccggca agcctaccgg catagtgcga ttaagataac gttgatgcag
     4381 gcagtaaacc gtcagcacca gggaaataat cgggaataca gaaaatggca caatggagct
     4441 aaacagcgct gcgaaagtac cattaatcga taagccagca actaacgcca gcagcagcgt
     4501 acctctatct tgatttgctt gtttcattac tcgtccttca catttcccgg aataattgcg
     4561 gttttctctt gttcacggcg ataccagtaa taagcccctt tcgaaatcat tcgcagttgc
     4621 agtaccagtc gctcttctaa ttgccgacgt tgttcgacgc cgacgtccaa cgcctcggca
     4681 cccgcactga agacaattgt caccattgct tcggcttgcg cttcagtaaa cgcacgcggc
     4741 atatggtttt cgagttccag atagtccgca agttccgcaa tgaagtgctg aatttcacgc
     4801 gcaacggcgg cacgaaacgc agcggaggtg ccggagcgtt cccgcaataa taaccggaag
     4861 gcgttaggat tattaccgat gaactccata aatgtggaga ccgaggtgcg gatcacactc
     4921 ccgcctttgg cgatacgctg acgcgcctgg cgcatgagtt ggcgtagcat taaaccgctc
     4981 tcatcaacca tggtcagacc cagttcgtct acgtcgcgga aatgccgata aaaagaggtg
     5041 ggagcaatgc ccgcttcacg cgccacttca cgcaaactca ggctggcgaa gctgcgttca
     5101 gcacttaatt ggctaaatgc ggcttccacc agcgaacggc gggttttttc tttttgttgc
     5161 gctcttacgc ccatcacgat gtctgaatcc ttgccaaaag tactgctggc actataccag
     5221 agaatgaaca tactggattg ctccagtatt gtgaatgaac ggtaacgcaa taaaacatat
     5281 ttactgcaat tctttggccg gttcttttac gtacagcgga aacctgccgc ttaaacggag
     5341 agtatcgtcg ataaaaatcc aataaaacgt cagggtaaaa gtaagaaaca gacaaagcaa
     5401 aggccgctca ggatatagcc agataaatga cggggatcaa ttggcttacc cgcgataaaa
     5461 tgttaccatt ctgttgcttt tatgtataag aacaggtaag ccctaccatg ccacattcct
     5521 acgattacga tgccatagta ataggttccg gccccggcgg cgaaggcgct gcaatgggcc
     5581 tggttaagca aggtgcgcgc gtcgcagtta tcgagcgtta tcaaaatgtt ggcggcggtt
     5641 gcacccactg gggcaccatc ccgtcgaaag ctctccgtca cgccgtcagc cgcattatag
     5701 aattcaatca aaacccacta tacagcgacc attcccgact gctccgctct tcttttgccg
     5761 atatccttaa ccatgccgat aacgtgatta atcaacaaac gcgcatgcgt cagggatttt
     5821 acgaacgtaa tcactgtgaa atattgcagg ggaacgctcg ctttgttgac gagcatacgt
     5881 tggcgctgga ttgcccggac ggcagcgtcg aaacactaac cgctgaaaaa tttgttattg
     5941 cctgcggctc tcgtccatat catccaacag atgttgattt cacccatcca cgcatttacg
     6001 acagcgactc aattctcagc atgcaccacg aaccgcgcca tgtacttatc tatggtgctg
     6061 gagtgatcgg ctgtgaatat gcgtcgatct tccgcggtat ggatgtaaaa gtggatctga
     6121 tcaacacccg cgatcgcctg ctggcatttc tcgatcaaga gatgtcagat tctctctcct
     6181 atcacttctg gaacagtggc gtagtgattc gtcacaacga agagtacgag aagatcgaag
     6241 gctgtgacga tggtgtgatc atgcatctga agtcgggtaa aaaactgaaa gctgactgcc
     6301 tgctctatgc caacggtcgc accggtaata ccgattcgct ggcgttacag aacattgggc
     6361 tagaaactga cagccgcgga cagctgaagg tcaacagcat gtatcagacc gcacagccac
     6421 acgtttacgc ggtgggcgac gtgattggtt atccgagcct ggcgtcggcg gcctatgacc
     6481 aggggcgcat tgccgcgcag gcgctggtaa aaggcgaagc caccgcacat ctgattgaag
     6541 atatccctac cggtatttac accatcccgg aaatcagctc tgtgggcaaa accgaacagc
     6601 agctgaccgc aatgaaagtg ccatatgaag tgggccgcgc ccagtttaaa catctggcac
     6661 gcgcacaaat cgtcggcatg aacgtgggca cgctgaaaat tttgttccat cgggaaacaa
     6721 aagagattct gggtattcac tgctttggcg agcgcgctgc cgaaattatt catatcggtc
     6781 aggcgattat ggaacagaaa ggtggcggca acactattga gtacttcgtc aacaccacct
     6841 ttaactaccc gacgatggcg gaagcctatc gggtagctgc gttaaacggt ttaaaccgcc
     6901 tgttttaaaa ctttatcgaa atggccatcc attcttgcgc ggatggcctc tgccagctgc
     6961 tcatagcggc tgcgcagcgg tgagccagga cgataaacca ggccaatagt gcggcgtggt
     7021 tccggcttaa tgcacggcag ataaacaacc ccatcgcgtt tgcgctccgg cggcacagcc
     7081 agcgctggca gtaaagtgat cccgctacct gccgccacca tgttgcgcag agtttccagg
     7141 ctggtcgcgc ggaagtgtgt atcttcatcc gccccggctt caaaacagaa acccattgcc
     7201 tgatcgcgca aacagtgacc atcttccagc atcagcagtt tttcccctgc cagatcggcc
     7261 atcggtacgc attcgcggtt cgcccacggg tgatcttcat agatagccag caacattggc
     7321 tcatcaaaca acggcacttc aatgaatgct tcgctctctt tcaccagcgc gaggatcacg
     7381 caatcgagtt tgccgctgtc cagttgcgcc agtaactggt gggtctgtgc ttcatgcaga
     7441 tacatttcca gctttggaaa ggtctggtgc agcataggga taatatgcgg tagcaggtac
     7501 ggtccaactg tgggaatcaa accaatgtgc agcggtccgg acatcgtctc gccctgctgg
     7561 cttgccatct ctttaaggac tttcacctca cgcagcacgg tacgcgcctg atccaccagc
     7621 agcattcccg cctgggtgaa caacacttta cggctggtcc gctccagcaa catcacgccc
     7681 agctcatctt ccagcttacg aatttgcccg ctaagcgtcg gctggctaac gtggcaggaa
     7741 tctgccgcac gccgaaaatg gcggtgttca gccaatgcca ccaggtactc aagatcacga
     7801 atattcatta tccatcctcc atcgccacga tagttcatgg cgataggtag aatagcaatg
     7861 aacgattatc cctatcaagc attctgactg ataattgctc acagaaacgg agcggcacct
     7921 cttttaaccc ttgaagtcac tgcccgtttc gagagtttct caactcgaat aactaaagcc
     7981 aacgtgaact tttgcggatc tccaggatcc gctttttttt gccataaaaa agcccggcga
     8041 taagccaggc tcaaatttat acatataaat gttcttaccc taaccgagcc tgcgcaaaag
     8101 caatcgcctg cgccacctgc tgcggtgaga cgccgccttt tgccgcacgc ttgtcgaggc
     8161 acgattgcag cgacagaatc ggatagacat cttcgtcaat cacctgactg aatttctgca
     8221 actcactgag cggcagatct tccagcggtt tgccctgacg aatggcttcc accaccgctt
     8281 caccaacaat atggtgcgcc tcgcggaacg gtacgccttt cgccaccaga taatccgcca
     8341 gttcggtggc gttggcgtaa ccctgctgag ccgcttcctg gcaacgtgga cgtttcacct
     8401 gaatgccgtc cagcaccagc gccgccatat gcaggcagtc cagccaggta tcgagcgcgt
     8461 cgaacagacc ttctttgtct tcctgcatat ctttgttgta agccagcggc aaacctttca
     8521 gcgtcatcat catgccggtt aacgccccct gcacccggcc gcatttaccg cgaatcagct
     8581 ccagcgcatc cgggtttttc ttctgcggca ttaatgatga accggaagtc acgcggtcag
     8641 aaagctccac aaaccccgct tcgccggtgt taaagaaaat cagatcttca gcaaaacgcg
     8701 acagatgcac catgccgata gcggcagcag aaagcagttc caacacatgg tcacggtcag
     8761 aaacgctgtc gagactgtta cgggtcgccg aagcaaagcc cagccagcct gctaactgtt
     8821 cacggtcgat ttcataggcc gttcccgcca gcgcgccaca gcctagcggg ctgacatcca
     8881 gacgcttaag cgcatcctgc aaacggcttt catcacgcgc cagcatctca acataggcca
     8941 ggcaccagtg cgcgaacgtc accggctggg cgcgttgcag gtgagtgtaa cctggcatta
     9001 ccgcgtcctg attgttttgt gcggtttcca ccagcgccga ttgcagctgc cggttagccg
     9061 tcagtaactc gctaacggta tctttgcacc acagtttcag gtcagtcgct acctgatcat
     9121 tacggctacg cccggtatgc agctttttgc ctaactggcc cactttgtcg atcagtttgc
     9181 cttccaccca gctatggata tcttcggcgt cgctttcaag gatttgttgt ggcctggcgc
     9241 gaacatcttc cagcaacacg ttcagcgcct cttccagttg cgcctgctct tctgcggtta
     9301 acacgcctac cgtgaccagg gctttggacc aggccacaga gccaacaata tcctgctccg
     9361 ccagacggta atcaaagcgc agtgagtcgt tgaattgttt gaaccgttga tctgctgcct
     9421 gggtaaaacg cccgccccaa agtgccataa ctctgtttcc ttattttgaa attcaatgcc
     9481 ggaaagctgc gcctccggcc aacaaaactt aagctaaaat ccgcgtaccc atcggcatac
     9541 cgttaaacag tgccggaagc tgctccgcat gacgccagga ggcgatatct accggacggc
     9601 ccagcgtgcg ggccgcatcc agcgccgcgt tcactttcac tatcatgccg tcagtaataa
     9661 tgccctgctc aatcagttgt tctgctttcg cggcggtcat ttcggcaatg cgttgccctt
     9721 tgccgtcgag aatgccgctg acgtcggaga gcaaaatcag atccgcgccc agcgttgccg
     9781 ccagcgccgt tgccgcctgg tcggcattga cgttcatcag ttgcccttcg tctgttacgc
     9841 caatggagct gaccaccggc agataaccgt tctccagcaa ggagttgata agcttaggcg
     9901 aacctggctg cgccagtcca acatgaccta actcttcatc aagctgggtc actttgacgc
     9961 tgtcgccgtc accgagaaac aaacctacgg ccgcaatctg atgtttcttc gcccatgcca
    10021 acagggtttt atttgccgtt cccgccagtg ctccggtgat aatgtctatc tgatcagcag
    10081 gcgtcacccg caggccgttt ttctttttca ccggcagatt cagccctttc atcagctcat
    10141 ccaccacgca accgccgccg tgcacaatca ccagcggacg ctgatgtgac tcacgataat
    10201 tcaccagtgc gctaaacaga cgttccagcg cctcttcact atccagcagt acgccgccca
    10261 gtttgataat taatggattc atcattgcac ccttaaataa gagactgcgt ttccgcatag
    10321 ccgaaacgaa tattggcgca ctgtaccgct tgtgccgccg cgcctttcag taagttgtct
    10381 tcggtcgcca caataatcag atgctcgccc tgaacggcaa acccgatatc gcaaaatggc
    10441 agcccaacga catttttcag cgccggaacg cctttgtcat acagccgcac cagcggttta
    10501 tgggcatacg cctgctgtaa cacttgcgcg acttgcgcct gggtcacacc cgatttcagg
    10561 cggcaggtaa tggtttcgag aatgccgcgc gggaaattgc ccagatgtgg ggtgaagata
    10621 acgtcagcac cgaggtgtgt ggcgatctct ggttgatggc gatgagtaaa gacgccatac
    10681 ggttgcaggc taacttcaca aaagctgttt gaaatggccg ctttacgccc tgcaccgctc
    10741 acgccgctgg tggcgttgat caccggccac tgattgaggt caagaagatc ggcatcaatc
    10801 aacggtttca gcgccagctg tgccgccgtc ggataacagc ccggcaccgc aatcaaattc
    10861 gcttctttta atttattacc gcaccactcc gccagaccgt aggctgcctg ttccaacagt
    10921 tccgggtatt gatgggtaaa gccgtaatat ttttcataga aggtggcgtc gttaacacga
    10981 aacgcgccgg aaaggtcgaa caccacgcag cccgcttcaa gaaactgcgg cgctaaatcg
    11041 tggctaactt catgggcggt ggcgagaaac actacgtcca cccctgggct aaactcgctg
    11101 atatccgaca tcggctgcaa cggcagatca acgatgcctt ttagctgcgg atgcaaatcg
    11161 gagattaact ttcccgcatc attgctttgc gctgaaacag tcaaagcggt tatgttcata
    11221 tgcggatggc gatttacata ggtcactagc tctgcgccag cgtagccgct ggcacccaca
    11281 atcagcgtat tcaacatcgg ggctattcac cttcttatgt ctggttgcca ggttaaacgt
    11341 aaaacattca ccttacggct ggtgggtttt attacgctca acgttagtgt atttttattc
    11401 ataaatactg catgaatatt gatactatca tgaccagagg tgtgtcaaca atgaaaaaca
    11461 aattaccgcc atttatcgag atttaccgcg ctctgattgc cacaccttca ataagcgcca
    11521 cggaagaggc actcgatcaa agcaatgcag atttaatcac tctgctggcg gactggttta
    11581 aagatttggg cttcaatgtg gaagtgcagc ctgttccagg aactcgcaac aaatttaata
    11641 tgctggccag ttgcggacag ggggctggcg gcttgttgct ggcggggcat accgatacgg
    11701 tgccatttga tgacggtcgc tggacgcgcg atccgtttac actgacggag catgacggca
    11761 agctttacgg cttaggcacc gccgacatga aaggcttttt tgcgtttatc cttgatgcgc
    11821 tacgcgatgt cgacgtcacg aaactgaaaa aaccgctcta cattctggcg actgctgatg
    11881 aagaaaccag tatggccgga gcgcgttatt ttgccgaaac taccgccctg cgcccggatt
    11941 gcgccatcat tggcgaaccg acgtcactac aaccggtacg cgcacataaa ggtcatatct
    12001 ctaacgccat ccgtattcag ggccagtcgg ggcactccag cgatccagca cgcggagtta
    12061 acgctatcga actaatgcac gacgccatcg ggcatatttt gcaattgcgc gataacctga
    12121 aagaacgtta tcactacgaa gcgtttaccg tgccataccc tacgctcaac ctcgggcata
    12181 ttcacggtgg cgacgcttct aaccgtattt gcgcttgctg tgagttgcat atggatattc
    12241 gtccgctgcc tggcatgaca ctcaatgaac ttaatggttt gctcaacgat gcattggctc
    12301 cggtgagcga acgctggccg ggtcgtctga cggtcgacga gctgcatccg ccgatccctg
    12361 gctatgaatg cccaccgaat catcaactgg ttgaagtagt tgagaaattg ctcggagcaa
    12421 agaccgaagt ggtgaactac tgtaccgaag cgccgtttat tcaaacgtta tgcccgacgc
    12481 tggtgttggg gcctggctca attaatcagg ctcatcaacc tgatgaatat ctggaaacac
    12541 ggtttatcaa gcccacccgc gaactgataa cccaggtaat tcaccatttt tgctggcatt
    12601 aaaacgtagg ccggataagg cgctcgcgcc gcatccggca ctgttgtcaa actccagtgc
    12661 cgcaataatg tcggatgcga tacttgcgca tcttatccga cctacacctt tggtgttact
    12721 tggggcgatt ttttaacatt tccataagtt acgcttattt aaagcgtcgt gaatttaatg
    12781 acgtaaattc ctgctattta ttcgtttgct gaagcgattt cgcagcattt gacgtcaccg
    12841 cttttacgtg gctttataaa agacgacgaa aagcaaagcc cgagcatatt cgcgccaatg
    12901 cgacgtgaag gatacagggc tatcaaacga taagatgggg tgtctggggt aatatgaacg
    12961 aacaatattc cgcattgcgt agtaatgtca gtatgctcgg caaagtgctg ggagaaacca
    13021 tcaaggatgc gttgggagaa cacattcttg aacgcgtaga aactatccgt aagttgtcga
    13081 aatcttcacg cgctggcaat gatgctaacc gccaggagtt actcaccacc ttacaaaatt
    13141 tgtcgaacga cgagctgctg cccgttgcgc gtgcgtttag tcagttcctg aacctggcca
    13201 acaccgccga gcaataccac agcatttcgc cgaaaggcga agctgccagc aacccggaag
    13261 tgatcgcccg caccctgcgt aaactgaaaa accagccgga actgagcgaa gacaccatca
    13321 aaaaagcagt ggaatcgctg tcgctggaac tggtcctcac ggctcaccca accgaaatta
    13381 cccgtcgtac actgatccac aaaatggtgg aagtgaacgc ctgtttaaaa cagctcgata
    13441 acaaagatat cgctgactac gaacacaacc agctgatgcg tcgcctgcgc cagttgatcg
    13501 cccagtcatg gcataccgat gaaatccgta agctgcgtcc aagcccggta gatgaagcca
    13561 aatggggctt tgccgtagtg gaaaacagcc tgtggcaagg cgtaccaaat tacctgcgcg
    13621 aactgaacga acaactggaa gagaacctcg gctacaaact gcccgtcgaa tttgttccgg
    13681 tccgttttac ttcgtggatg ggcggcgacc gcgacggcaa cccgaacgtc actgccgata
    13741 tcacccgcca cgtcctgcta ctcagccgct ggaaagccac cgatttgttc ctgaaagata
    13801 ttcaggtgct ggtttctgaa ctgtcgatgg ttgaagctac ccctgaactg ctggcgctgg
    13861 ttggcgaaga aggtgccgca gaaccgtatc gctatctgat gaaaaacctg cgttctcgcc
    13921 tgatggcgac acaggcatgg ctggaagcgc gcctgaaagg cgaagaactg ccaaaaccag
    13981 aaggcctgct gacacaaaac gaagaactgt gggaaccgct ctacgcttgc taccagtcac
    14041 ttcaggcgtg tggcatgggt attatcgcca acggcgatct gctcgacacc ctgcgccgcg
    14101 tgaaatgttt cggcgtaccg ctggtccgta ttgatatccg tcaggagagc acccgccata
    14161 ccgaagcgct gggcgagctg acccgctacc tcggtatcgg cgactacgaa agctggtcag
    14221 aggccgacaa acaggcgttc ctgatccgcg aactgaactc caaacgtccg cttctgccgc
    14281 gcaactggca accaagcgcc gaaacgcgcg aagtgctcga tacctgccag gtgattgccg
    14341 aagcgccgca aggctccatt gccgcctacg tgatctcgat ggcgaaaacg ccgtccgacg
    14401 tactggctgt ccacctgctg ctgaaagaag cgggtatcgg gtttgcgatg ccggttgctc
    14461 cgctgtttga aaccctcgat gacctgaaca acgccaacga tgtcatgacc cagctgctca
    14521 atatcgactg gtatcgcggc ctgattcagg gcaaacagat ggtgatgatt ggctattccg
    14581 actcagcaaa agatgcgggc gtgatggcag cgtcctgggc gcaatatcag gcacaggatg
    14641 cattaatcaa aacctgcgaa aaagcgggta ttgagctgac gttgttccac ggtcgcggcg
    14701 gttccattgg tcgcggcggc gcacctgccc atgcggcact gctgtcacaa ccgccaggaa
    14761 gcctgaaagg cggcctgcgc gtaaccgaac agggcgagat gatccgcttt aaatatggtc
    14821 tgccagaaat caccgtcagc agcctgtcgc tttataccgg ggcgattctg gaagccaacc
    14881 tgctgccacc gccggagccg aaagagagct ggcgtcgcat tatggatgaa ctgtcagtca
    14941 tctcctgcga tgtctaccgc ggctacgtac gtgaaaacaa agattttgtg ccttacttcc
    15001 gctccgctac gccggaacaa gaactgggca aactgccgtt gggttcacgt ccggcgaaac
    15061 gtcgcccaac cggcggcgtc gagtcactac gcgccattcc gtggatcttc gcctggacgc
    15121 aaaaccgtct gatgctcccc gcctggctgg gtgcaggtac ggcgctgcaa aaagtggtcg
    15181 aagacggcaa acagagcgag ctggaggcta tgtgccgcga ttggccattc ttctcgacgc
    15241 gtctcggcat gctggagatg gtcttcgcca aagcagacct gtggctggcg gaatactatg
    15301 accaacgcct ggtagacaaa gcactgtggc cgttaggtaa agagttacgc aacctgcaag
    15361 aagaagacat caaagtggtg ctggcgattg ccaacgattc ccatctgatg gccgatctgc
    15421 cgtggattgc agagtctatt cagctacgga atatttacac cgacccgctg aacgtattgc
    15481 aggccgagtt gctgcaccgc tcccgccagg cagaaaaaga aggccaggaa ccggatcctc
    15541 gcgtcgaaca agcgttaatg gtcactattg ccgggattgc ggcaggtatg cgtaataccg
    15601 gctaatcttc ctcttctgca aaccctcgtg cttttgcgcg agggttttct gaaatacttc
    15661 tgttctaaca ccctcgtttt caatatattt ctgtctgcat tttattcaaa ttctgaatat
    15721 accttcagat atccttaagg aattgtcgtt acattcggcg atattttttt caagacaggt
    15781 tcttactatg cattccacag aagtccaggc taaacccctt tttagctgga aagccctggg
    15841 ttgggcactg ctctactttt ggtttttctc tactctgcta caggccatta tttacatcag
    15901 tggttatagt ggcaccaacg gcattcgcga ctcgctgtta ttcagctcgc tgtggttgat
    15961 cccggtattc ctctttccga agcggattaa aattattgcc gcagtaatcg gcgtggtgct
    16021 atgggcggcc tctctggcgg cgctatgcta ctacgtcatc tacgggcagg agttctcgca
    16081 gagcgttctg tttgtgatgt tcgaaaccaa caccaacgaa gccagcgagt atttaagcca
    16141 gtatttcagc ctgaaaattg tgcttatcgc gctggcctat acggcggtgg cagttctgct
    16201 gtggacacgc ctgcgcccgg tctatattcc aaagccgtgg cgttatgttg tctcttttgc
    16261 cctgctttat ggcttgattc tgcatccgat cgccatgaat acgtttatca aaaacaagcc
    16321 gtttgagaaa acgttggata acctggcctc gcgtatggag cctgccgcac cgtggcaatt
    16381 cctgaccggc tattatcagt atcgtcagca actaaactcg ctaacaaagt tactgaatga
    16441 aaataatgcc ttgccgccac tggctaattt caaagatgaa tcgggtaacg aaccgcgcac
    16501 tttagtgctg gtgattggcg agtcgaccca gcgcggacgc atgagtctgt acggttatcc
    16561 gcgtgaaacc acgccggagc tggatgcgct gcataaaacc gatccgaatc tgaccgtgtt
    16621 taataacgta gttacgtctc gtccgtacac cattgaaatc ctgcaacagg cgctgacctt
    16681 tgccaatgaa aagaacccgg atctgtatct gacgcagccg tcgctgatga acatgatgaa
    16741 acaggcgggt tataaaacct tctggatcac caaccagcag acgatgaccg cccgcaatac
    16801 catgctgacg gtattttcgc gccagaccga caagcagtac tacatgaacc agcaacgtac
    16861 gcagagtgcg cgtgaatacg acaccaacgt gctgaagccg ttccaggatg tgctgaatga
    16921 ccctgcgccg aagaaactga tcatcgttca tctgctgggt acgcatatca aatacaaata
    16981 ccgctacccg gaaaatcagg gcaagtttga tggcaatacc gatcatgttc cgccaggatt
    17041 aagcgcagaa gagctggaat catataacga ttatgacaac gctaacctgt ataacgatca
    17101 tgtggttgcc agcctgatta aagactttaa agcggcagac ccgaacggat tcctggttta
    17161 cttctctgac cacggtgaag aggtttacga cacgccgccg cacaaaactc aggggcgtaa
    17221 cgaagacaac ccgacgcgcc acatgtacac cattccgttc ctgctgtgga cgtcggaaaa
    17281 atggcaagcg actcatcccc gtgatttctc acaggatgtc gatcgtaaat acagcctggc
    17341 ggaactgatc cacacctggt cagatttggc gggcttatct tacgacggtt acgatccaac
    17401 ccgttcagtg gtgaatccgc agttcaaaga aactacccgc tggattggta acccgtacaa
    17461 gaaaaacgcg ctgatcgatt acgacactct gccgtatggc gaccaggtag gtaatcagta
    17521 attatcagcc aggcgctatt tgccggatgc gacgcttgac gcgtcttatc cggcctacag
    17581 atcgcggcgg taggctggat aaggcgttta cgccacatcc ggcaatacaa ctgccgcaat
    17641 gtttacatcg aactcccctc aggccacact tttcaggtgg ccttttttaa taatgccagt
    17701 tactgttttc ccgtctacat acctaaggga ataacatgta tcacgacgtc agctatctgc
    17761 tctcccgcct gatcaacggc ccgctgtccc tgcgccagat ttactttgcc agcagtaacg
    17821 gcccggtgcc cgatcttgcg tatcaggtcg attttccacg gctggaaatt gtgctggaag
    17881 gtgagtttgt tgataccggc gctggagcaa cgttagttcc cggcgatgtg ctgtacgttg
    17941 ccgctggtgg ctggaatttc ccacaatgga aaacacccgc taccaccttt agcgtgctgt
    18001 ttggcaaaca gcaactcggc ttcagcgtcg tgcaatggga tggcaaacaa tatcaaaatc
    18061 tggcgaagca acacgtcgcc cggcgcggcc cacgcatagg ttcttttctg ctacaaacgc
    18121 tcaatgaaat gcaaatgcag ccgcaggagc agcaaacggc aaggcttatc gtcgccagcc
    18181 tgcttagcca ctgccgcgat ttgcttggca gccaaatcca gaccgcctca cgcagccagg
    18241 cactatttga agctattcgc gattatatcg acgaacgcta tgcctccgcg cttacccgcg
    18301 aatctgttgc gcaggcgttt tatatttcgc caaattacct ctcgcacctg tttcaaaaaa
    18361 cgggggccat tggttttaac gaatacctga atcacacgcg actggagcac gctaagacgt
    18421 tattgaaagg ctatgacctg aaggtaaaag aagtggcgca cgcctgcggc tttgtcgaca
    18481 gcaactactt ctgccgtctg tttcgtaaaa acaccgaacg ctcgccgtcg gaatatcgtc
    18541 ggcagtacca cagccaactg acagaaaaac cgactactcc agaataagat gggtttgctg
    18601 cggcgcagaa agcactttgc gcaccgcgct cattacccgc tgcggctcac gcaggaatgc
    18661 gtagatgctg cattgcacat agcggcaatg ttcaaatcgc tcggcaccgg caagctcgat
    18721 atccgtaatc aacagagcaa catccgcccg acggatatcc tcgtccgcta aacgattctc
    18781 cgttcccagc gcgccctgag tttcaatgct gactccccac ttctctaaca ggcacaactt
    18841 ttccagccgt tccgccgcca tataagtatg cgccacgcca ctgacgcagg cggttactgc
    18901 caccaggtat gccattttaa cctcccacgg taacctgaaa tccggcccgt tctgccattt
    18961 cgcgcatcgt tgccacatcg gctgacgacg gcgcaggcac ctctttcatc gaccatgttt
    19021 tccccagcag gcggtatttc ggttcgccgt actgatgaaa cggtaacaga tggatctgcc
    19081 tgatattcag cgggatcagc acatccagcg cctgctgcat attctcccgg ctgagcgtga
    19141 aaccagggat cagcggtaaa cgcgggatca cgttgacgcc ctcactcacc agcaaacgca
    19201 gattctccag cacgcgtggc aggttcatct tcaccacatc ccgcgcctga gtcgcgtcca
    19261 tgatttttaa atcgaacaac acttcatcgc acaatttcgc cagcggtaac agcttgctgg
    19321 ctggcgcgtc tccggcagtt tcaatggcac atgagacacc ccacagccgc agtcgctgta
    19381 aaaaacgggt agcaaactcc gcctgcatta acacttcgcc gccagaaagc gtgacgccgc
    19441 cgccggacgt gcgaaaaaaa atgtcatctt tcatcacttc ccgttccagc gcgtcaaggc
    19501 tgatatcgcg accaatccgt tcaaacgccc cggaggggca ttcatccgca tcacgcaaac
    19561 atttcgcaca gtgcagacat ttcgcctctc tgcgtaccgt ctggattttg ccggagatcg
    19621 actccggatt agcacaccac gggcaaagat gcggacagcc tttaaaaaag accaccgtac
    19681 gaatgccctc gccgtcattc agtgaataac gctgaatgtt gaaaatgcgc gccacatcat
    19741 tgcggcgcgt ttccacaacg ttacagctga tgcgctgtcc ggcggatgat gtcatcctgg
    19801 atctccttcg acagttcgac aaagaaggcg ctgtatccgg caacgcgcac caccagcccg
    19861 gcataatctt gtgggcgctg ttgcgcttcc cgcaacgtgt cggcgttcac cacgttaaac
    19921 tgaatatgct gcaacttaag ctgggtaaac gcccgtaaga agtcggccag tttgcgtaat
    19981 cccgcttcac cttccagggt cgccggagtg aatttcacgt tcagcaacgt accgttagac
    20041 agcagcgtat tatcgagctt actgactgac ttcagtaccg ctgttggccc ttgttcgtcc
    20101 tggcccagca taggtgacaa gccgccgtct gccagctgtt ctccggcaaa acgaccgtct
    20161 ggcgtcgcgc caaccaccga tcccaacggg acgtgagcag aaacggtata cgatcccggc
    20221 gtgaagtaac caccgcgcgg gttctggtat ttttccactt ctttgcagta gtggcgcaac
    20281 agttcggcgc taatgttatc cacctcgtcg atatcgttac cgtatttctc aaagcggtta
    20341 attaagcgag cgcggacttt ttcgccttct ggcgttgcaa agttggcttt taataccgac
    20401 agcaattcgt caaaacttaa acgctgttgc tcaaaaacca tccctttcaa cgcatggaga
    20461 gaatcgctca ggttggcgat accgatcccc tgtacgccgg agaagttata acgcgcgccg
    20521 ccatcggtaa tatcgcgacc tttttccaga caatcgctga taaaagacga gagcagcggt
    20581 acaggtgccc agtcgcgatg gccgatatcg caaatattac tgccttcaac catcagggtg
    20641 atgtagtggc tgatcttggc acggatctgt tccagtaaac cttcataagt cagcgcggca
    20701 ttgccttcat tttcatgcag gcagatttcc atcactttca gcaggttaaa catcgcgatg
    20761 tcatgcaagc cgtaggttct gccgggaata gataattcca cacagcccac tacggaatag
    20821 tcgcgcgcat cttccagcga aacgccacgg ttgaggaacg ctggcaccac cacttcatcg
    20881 ttaaagattt gcggaatacc ggtgccgagg cgaatggttt cggcggtttt catcaggaac
    20941 ggcgtgtcga tcaaggcgtt agtgcgcacg ccgaggttcg gttgcggtaa ttgcacgctt
    21001 tgataggcgt caaggcaaag gaacgaaagc acgttcaccg cgctacgtcc gttctcggtt
    21061 aacccgccga gcagtgcggt atagccggtc gggaaaccgg cgaaataacg cgcgctgctg
    21121 gtggagcgca acagcacgat gtcgttgcat ttcacccata aagattcgag cagttctttc
    21181 aggaacgccg catcttcgcc ctgggttaat gatgtctgat agaacggcaa catatactgg
    21241 tcgaagcgcc ccaacgatag cgaactggcg ttggattcgt attgcagaat gatgttcatg
    21301 taccagaata actggcacgc ctgccagaac gtctgcggct tatgttgcgc gttatggcgg
    21361 gagatctccg caatagtcag cagctcttcg cgacgctggg catctgtgca gtttgccgcc
    21421 atcgtttccg ccagttcggc gtaacgcaaa atgtgtttct gcgaggcttc cagcagtaac
    21481 agcgctgcct gataaaagtg attctccggc tgttgctgac aatgttgctg catctgtgct
    21541 accagctccc ccagcccgtg attcagcagg cgtgggtaat caataataat gtgcccctgg
    21601 cctttatctg tctggttgat gctgaaaatc tgcgtgctgg tcgcggcttt tacttcatct
    21661 gtcatctgcc cgttgatgaa atctttcatc gaacgttttt cccagtacgg gaacaactct
    21721 tcgcggtaga tacgtttatc ttcttcgctg atagcaaagc ggtcctgcgg acgcgtcggg
    21781 aattgatcca gctctttcag cagccagtaa gggtccattt ccggcgacat aatcccggcg
    21841 cgcggtttta cggtgcggtt accggcaatc agttcttcat cacgaatcga aatttcaaca
    21901 tgttcaagga tatacgctgt tgctttcgcc cggcgcaata tcaccggttc gccttcggtt
    21961 tgccgatggc tggcggtata aagcagcgcc cgctccagcg agatttcacg ggtattggaa
    22021 aacagtgcag ttttgaggcg agagatacga ttcgtcatga ggaactcctg tgggaaagcg
    22081 aacccgccct tgcgagcggg agaagaactt aagcggtttg cgccaggtga gcttcaattt
    22141 tgctcataat ggcgtcagca cgttttaccg catcgctgat gttgacgcgc acgatggtct
    22201 tgccagcaaa acgctcttca aacttgatgc cgatatcttt ggtcagaatc accatatcag
    22261 cgctcgccac gtcttccgct gtcagttcat tttccagacc aattgacccc tgggtttcta
    22321 ctttcacttc ccagcctttc gctttcgcgg cactttccag cgcctctgct gccatatagg
    22381 tatgggcaac acctgaagga catgcggtta ctgcaataat tttcgtcatg gcttgttcct
    22441 caattaatta atttcaaaat ccaaatccag gtcgtcttct ttttcatcag tcgaagaccc
    22501 atttttacgc gccagacttt tcagcacgtt cacacaaaca gccgtcacca ctgctccgac
    22561 agccactgcg gcgatataac ccagcttgcc ttcaaccacc ggcagtacaa tcagaccacc
    22621 ccagcctgca tagcactgcg caccgaccag cgccgcagtt accgcaccac aaacagaacc
    22681 aaccatgatg gaaggaatga cacgcagcgg atcggcagcg gcgaaaggaa tcgccccttc
    22741 agtaacccca acgcacccca tcaccagcgc cgctttgccg gtttcgcgct cttctgcgga
    22801 gaaattttta cgaccaatca gcgtcgccag ccccatcccc agcggcggga tacaaatacc
    22861 cacggcagca atggccacca cggtataaac accctgagca acgcaaatca gcatgaaggc
    22921 ataggccact ttgttaaccg gaccgcccat atcgaacgcc agcatcagac ccatgatcac
    22981 cgccagcata acaatgctgc cctgctgcat cccctgtagc cactgagtca ggctgttggt
    23041 caatgccccc accggctcgc ccaagcccca catcatgatg ccagcggtaa tcagcgtgcc
    23101 gacgatcgga atgatgaaga ttggcatcac cgagcgcaga actttatgca ccggaatttt
    23161 cttcaggtaa tgcaccacga tgccgccgat aatcccggcg atcagtgcac caaagaagcc
    23221 cgcaccaaag ctgttaccaa cccaggcacc gatagcgcac ggagccagcg cagaacgctc
    23281 tgcaatggag taaccaatgt aagcggcgag gaaaggcacc atcagcgtca aacctgcaac
    23341 gccgatatca aacagttttt tcaaattcgg atcggctacg gcatccggca ctgcgccttt
    23401 gccatacaac atgacggaaa ccgccagcaa aataccgccc gataccacga agggaatcat
    23461 gtgtgaaacg cccgtcatta aatgctgacg ggtatttttc aggatctgca ccaactcatt
    23521 cataagctgc tcccgggctt gcgaaagccc atgtccatat ttttgatgga ggaaacaata
    23581 ggcaatcgca ggcggagcaa acagtggata aaaaggccat ttactggact tttgtaatat
    23641 cagtacaaaa atgcgatccg cctcataact tgcgctaatc cggaagcatg ccgcaaaacg
    23701 gcgtcttgtg agacgcgtcg caaattgacg gttacattac atttgtccag tttttggcag
    23761 atttgtcaca tggcaggccg ttgccgtgcg ctcttatcct gtaaacagaa aaccgtaccg
    23821 ggagagaggc aatggccctg attgtggaat ttatttgtga gctacctaac ggcgtacatg
    23881 cgcgtccggc aagccacgtt gaaacgctgt gtaatacttt ttcatcacaa attgagtggc
    23941 ataacctgcg cactgaccgc aagggcaacg ccaaaagcgc ccttgcgctg attggcaccg
    24001 atacgctggt gggcgataac tgccagttac tgatttccgg ggccgacgaa caggaagcgc
    24061 accagcgttt aagccaatgg ctgcgcgatg aattccccca ctgcgacgca ccgctggcgg
    24121 aagttaaatc tgacgaactg gaaccactgc cggtttcact gaccaatctg aatccgcaaa
    24181 ttatccgcgc ccgcaccgtg tgcagcggta gtgcaggcgg cattctgacg ccgatctctt
    24241 ctttagatct caatgcgctg ggtaatcttc ccgcagccaa aagcgttgac gccgagcaat
    24301 ccgcgctgga aaacggcctg acgctggtac tgaaaaacat agagtttcgt ctgctggata
    24361 gcgacggtgc caccagcgcg atactggaag ctcaccgatc cctggctggc gatacatctt
    24421 tacgtcagca cttactggca ggcgtcagcg agggattaag ctgtgccgaa gcgattgtcg
    24481 ccagtgcgaa tcacttttgc gaagagttcg cccgttccag cagcagctac ctgcaagaac
    24541 gtgccctgga cgtacgcgac gtctgcttcc agttactcca gcaaatctac ggtgagcaac
    24601 gcttcccggc accgggcaaa ctgacgcagc ccgccatttg tatggctgat gaactgactc
    24661 ccagccagtt cctcgaactg gataaaaatc acctcaaagg tctgttactg aaaagtggcg
    24721 gcaccacctc acataccgta attcttgccc gttcgttcaa cattccgaca ctggttggtg
    24781 tagatattga tgcccttact ccgtggcagc atcaaacgat ttatatcgac ggcaacgccg
    24841 gggcgattgt ggttgagcca ggggaagccg tggcccgtta ttatcagcaa gaagcccgcg
    24901 tacaggacgc ccttcgtgag caacagcgtg tctggctgac ccaacaagcc cgtaccgctg
    24961 acggtatccg cattgaaatt gccgctaaca tcgctcactc cgtggaagcg caggccgcat
    25021 tcggcaatgg tgcggaaggc gttggtttgt tccgcactga aatgctctat atggatcgca
    25081 ccagtgcacc gggcgaaagc gagctgtaca acattttttg tcaggcgctg gagtctgcca
    25141 acggacgcag cattattgtg cgcactatgg acattggcgg cgacaaaccc gttgattatc
    25201 tgaacattcc ggcagaagct aacccgttcc tcggttatcg cgctgtgcgt atttatgaag
    25261 aatacgcctc gctgttcacc acacaactac ggtcgatcct gcgcgcctcc gctcacggca
    25321 gcctgaaaat catgatcccg atgatctcct caatggaaga gatcttatgg gtgaaagaaa
    25381 aactggcgga agccaaacag caactgcgta acgaacacat tccgtttgat gagaagatcc
    25441 agcttggcat tatgctggaa gtaccgtcgg tgatgttcat catcgatcaa tgctgcgaag
    25501 agattgattt ctttagtatt ggtagtaatg acctgacgca atatctgctg gcagtggatc
    25561 gcgataacgc taaggtgact cgtcactaca acagcctgaa tccggccttc ttgcgggcgc
    25621 tcgattacgc cgtgcaagcg gtgcatcgcc agggcaaatg gattggtctg tgcggtgagc
    25681 tgggagcaaa aggttccgtg ctgccgttgc tggtcggttt agggctggat gaactcagca
    25741 tgagcgcacc gtcaattccg gcggcgaaag cgcgaatggc gcaacttgat agccgtgagt
    25801 gccgcaagtt gctcaaccag gcaatggcct gccgtacttc gctggaagtg gaacatctgc
    25861 tggcgcaatt ccgcatgacc caacaggacg caccgctggt caccgccgag tgcatcacgc
    25921 tggaaagtga ctggcgcagc aaagaagaag tgctcaaagg catgaccgat aacctgctgc
    25981 tggcgggccg ctgccgctat ccccgtaaac tggaagccga cttatgggcg cgcgaggccg
    26041 ttttctctac cggtctgggc tttagttttg ccattccaca cagcaaatca gaacacattg
    26101 agcaatccac catcagcgtt gcgcgtctgc aagcgccggt gcgctggggt gatgatgaag
    26161 cgcaattcat catcatgtta accctgaaca aacacgctgc gggcgatcag cacatgcgca
    26221 ttttctcgcg cctcgctcgc cgcatcatgc acgaagaatt ccgtaacgcg ctggttaacg
    26281 ccgcctctgc cgacgctatc gccagcctgc tgcaacatga actggaactg taaaaggaaa
    26341 catcatggaa ctgtatctgg acaccgctaa cgtcgcagaa gtcgaacgtc tggcacgcat
    26401 attccccatt gccggggtga caactaaccc gagcattatc gctgccagca aagagtccat
    26461 atgggaagtg ctgccgcgtc tgcaaaaagc gattggtgat gagggcattc tgtttgctca
    26521 gaccatgagc cgcgacgcgc agggtatggt ggaagaagcg aagcgcctgc gcgacgccat
    26581 tccgggcatt gtggtgaaaa tcccggtgac ttccgaaggt ctggccgcca ttaagttgct
    26641 gaaaaaagag ggtattacta cacttggcac cgctgtatat agcgccgcac aagggttatt
    26701 agccgcactg gcaggggcaa aatacgttgc tccgtatgtt aaccgcgtag atgcccaggg
    26761 cggggacggc attcgtacgg ttcaggagct gcaaacgctg ttagaaatgc acgcgccaga
    26821 aagcatggtg ctggcggcca gctttaaaac gccgcgtcag gcgctggact gtttactggc
    26881 aggatgtgaa tccatcaccc tgcccttaga tgtagcgcaa caaatgctca acacccctgc
    26941 ggtagagtca gctatagaga agttcgaaca cgactggaat gccgcatttg gcactactca
    27001 tctctaaagg agcaattatg gaccgcatta ttcaatcacc gggtaaatac atccagggcg
    27061 ctgatgtgat taatcgtctg ggcgaatacc tgaagccgct ggcagaacgc tggttagtgg
    27121 tgggtgacaa atttgtttta ggttttgctc aatccactgt cgagaaaagc tttaaagatg
    27181 ctggactggt agtagaaatt gcgccgtttg gcggtgaatg ttcgcaaaat gagatcgacc
    27241 gtctgcgtgg catcgcggag actgcgcagt gtggcgcaat tctcggtatc ggtggcggaa
    27301 aaaccctcga tactgccaaa gcactggcac atttcatggg tgttccggta gcgatcgcac
    27361 cgactatcgc ctctaccgat gcaccgtgca gcgcattgtc tgttatctac accgatgagg
    27421 gtgagtttga ccgctatctg ctgttgccaa ataacccgaa tatggtcatt gtcgacacca
    27481 aaatcgtcgc tggcgcacct gcacgtctgt tagcggcggg tatcggcgat gcgctggcaa
    27541 cctggtttga agcacgcgcc tgttcccgta gcggcgctac cactatggcg ggcggcaagt
    27601 gtacccaggc ggcgctggca ctggcagaac tgtgctacaa cactctgctg gaagaaggcg
    27661 aaaaagcgat gcttgccgcc gaacagcatg tagtgactcc ggcgctggag cgcgtgattg
    27721 aagcgaacac ctatttaagc ggtgtaggct ttgaaagtgg tggtctggct gcggcgcacg
    27781 cagtgcataa cggcctgacc gctatcccgg acgcgcatca ctattatcac ggtgaaaaag
    27841 tggcatttgg tacgctgacg cagctggttc tggaaaacgc accggttgag gaaatcgaaa
    27901 ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa ctggatatta
    27961 aagaagatgt cccggcgaaa atgcgaattg tggcagaagc ggcatgtgca gaaggtgaaa
    28021 ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgct ctgctggtag
    28081 ccgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca aactcccggc
    28141 ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg cctacgcaat
    28201 ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc actccgcatc
    28261 cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggaattttt tatccgcttc
    28321 tggacatttt ctctacagaa caatcgttag cccggaaggt cgaatctgtg actcttattt
    28381 tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac ggcatttacc
    28441 agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc
    28501 aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt ttacgatccg
    28561 gcgtatgtgc agcttactta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    28621 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    28681 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    28741 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    28801 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    28861 gataacgggc tggcgcatat tggggtggtg tcagatggct tggcccgcga cggtacgccg
    28921 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    28981 atggtggggc attatcgtta ttttgttaaa taagcgatcg ccccacatga agaaaaaatt
    29041 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    29101 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    29161 gaaaagagta cgccgcaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    29221 aagtttttca gcacaaacag ccataagctc cacgccagcg ccgtcgccag aatcgcactg
    29281 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    29341 gcgaccacac tcatcaccag cgccgcatag agcatctgcc aggatgttaa cgataataaa
    29401 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    29461 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    29521 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    29581 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    29641 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    29701 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggtgtcgg gcgcattccg
    29761 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    29821 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    29881 cagataagtg tgagcacaac caggccactg attgccagtg ggttgctctt tcctgaggca
    29941 gacatgagag atccagtgtg tagttttcgt tcgcccacta tacgctggta aagaacgttc
    30001 agcgactgcc agcaagcagc cgctgaacgg ggtcagatta cagcaggtcg aaacggtcga
    30061 ggttcatcac tttcacccat gccgccacga agtctttaac aaacttctcg tgggcatcgc
    30121 tactggcgta aacttccgcc accgcacgca ggacggagtt agaaccaaac accagatccg
    30181 cacggctggc cgtaaatttc acttcgccgg tttcacggtc acggccttcg aacagctctt
    30241 tcgattcgtc ggtcgctttc cactcgtaac gcatatccag caagttcacg aagaagtcat
    30301 tgctcaatac gccaacgcgg tcagtgaaga cgccgttttt gctgccatcg aagttggcac
    30361 ccagtacacg catgccgccc accagcgcag tcatttccgg cgcggtcagc gtcagttgct
    30421 gtgctttgtc gatcagcagt gactcggtgg tggaaacgtc cagacgagcg cgatagttac
    30481 ggaaaccgtc agcaattggc tccagcagct caaacatctc aatgtcagtc tgatcctgac
    30541 gcgcatcaac gcgacccggc gcaaacggta catgaatgct caaacctgcg gcgcttgcgg
    30601 ctttctcaac accaaccaca ccagccagca cgatgatatc cgccagcgag gctttaccag
    30661 actctttctg gattttctcc agaacaggca gagcacgaac ggctgcggcg ttcacatccc
    30721 agtcgcgctg cggcattaat gccagacgcg caccgttggc accaccgcgt ttgtcgccac
    30781 cacggaaggt agaagcagat gcccaggcca ccgataccag ctcactaaca gacagaccag
    30841 aatccgcaat cgcgaatttc agatcgataa tgtcctgctc ggtcgggttg tagatcggct
    30901 gcggcagcgg atcttgccag atcagatctt ctttcggcac ttccggcccg atgtagcgag
    30961 atttcggccc catatccctg tgcgtcagtt tgaaccaggc acgggcaaag gcttcgttga
    31021 acgcctgcgg atcgttgagg aaacgacgag agatcttctc gaactcagga tcaaaacgca
    31081 gcgtcaggtc ggtcaccagc attgtcggtt tacgtttctt cgacggatca aacggatccg
    31141 ggataatttc cggtgcgtct accgcttcga actggattgc gccagccggg ctgcgggtct
    31201 gtacccactc atacttgaac aggttctcga agaaatagtt gctccactgg gtcggcgtct
    31261 gggtccagac tacttccaga ccagaggtaa tggcatctgc gccaacgccg ctgccgtaag
    31321 tgctcgccca acctaaacct tgttcttcaa tcggtgcagc ttctggatca ggacctacat
    31381 ttgatgtcgg accggcaccg tgggttttac ccagcgtatg accacccgca atcagcgcca
    31441 cggtttcttc gtcgttcatg cccatgttgc cgaaggtcgc gcggatagct gctgccgcag
    31501 aaagcggttc gccgctgtga tccgggcctt ccgggttaac gtaaatcaga cccatctcgg
    31561 ttgcacccag cggtgctttc gccagcgctt ccggatgacg gtgagtcagc caggcttttt
    31621 catcacccca gttaacatcc agatccggtt cccagacgtc ttcacgaccg gcaccaaaac
    31681 cgaaggtacg gaagccggag ttttctagcg ccacgttacc cgcgaggata aacaggtcgg
    31741 cccaggagat tttctgacca tatttctgtt tgattggcca caacaggcga cgcgctttat
    31801 cgaggcttac gttatccggc caggagttca gcggtgcaaa acgttgctga ccacgacccg
    31861 cgccaccgcg tccatcgatt gaacggtaag tccccgcgcc gtgccaggcc atacgaataa
    31921 acagaccggc gtaactgccc cagtcggctg gccaccacgg ttgagattct gtcaacaggg
    31981 ctttcagatc ttttttcagg ccgtagtaat ctaatttgct gaattctttg cggtagtcaa
    32041 agtcctcacc cagtgggtta gaacgattag aatgttggtt taacaggtca acacgaagtt
    32101 gatttggcca ccagtcgcga gtggttgtgc ccgcccccgc actctggtcg tgaccgccct
    32161 gatggaacgg gcatttgcca gtggctgtgg tgttatggat atcgtctgac gtgctcatca
    32221 atgtgctccc ctctacagtg ttaccgttac gatacacagc gttagagaga agttataatt
    32281 gaattaatcc acggatgcga tagttgagat cttacatatt gttggttaaa gagatgtaga
    32341 tcaaattgat cttaattaag cttttttgat tatgtccgaa ttcggacaaa tgctttataa
    32401 aaaagggttt tagcttcata tccttcaagt aatgagggag aaaacaaggc tgataacctt
    32461 attttcccgc cctcatttcg aggcagcatt ttgtgctctg tttaaaattt gtgatcactg
    32521 tgtgattttc acaaaagcca cactatttat aaaccaggtc gaacccccag cgtatggcaa
    32581 atcgcgtaac tcatttcagc acggttaagc gtatagaagt ggaaatcttt cactccttca
    32641 cggcttaaaa tcttcaccat atccatggca atattcgcgc caaccagttt gcgggtttcg
    32701 gcatcatcat ccagaccgtc gaacatttgc gccatccacg ccggaatacg cacgttggtc
    32761 atatcggcaa atttcttcgc ctgtttaaag ttagataccg gcaaaattcc cggaataatt
    32821 tccacatcaa tgcccgccga tacacagcgg tcacgaaaac gcaggtagct ttcgacatcg
    32881 aagaagaact gagtaatcgc gcggttggct ccggcatcca ctttgcgttt cagattaagc
    32941 aaatccgcct gagcgctttt tgcttccggg tgaacttccg gatacgccgc cacggagata
    33001 tcgaaatctg ccacttcttt taacagcgtc accaggtcag aagcatacat ttctggctta
    33061 ccacttcccg gcggcagatc gccacgcagc gccacgatat gacgaatacc gttattccag
    33121 tagtcgcgtg caatggtgcg cagctcgtcg ggcgtcgcat caatgcaagt aagatgcggt
    33181 gccgcttcca gaccagtgcg atctttaatg cctttaataa tgctgtgcgt acggtcgcgc
    33241 tcgccggagt tcgcgccata ggtcaccgat acaaacttcg gtttcaggct gctaaggcga
    33301 tcgatggagt tccacagggt ctgctccatt tcactggtac gcggcgggaa aaactcgaac
    33361 gaaacgttaa tctgcccctg gacttctgcc aggctctgat tcagggcatc ccgctggctg
    33421 gcgtgaaaaa agctcatacc ttacctcatc aatcgcttgt agttatatgt tgtgttgtgc
    33481 acatctatcc gtttagacgt ccagatgtaa agatgaagga aaagccgaag ggcgtcaacc
    33541 gaaaaatgac caatgatggt gaggaaaaat cagggaagat gaaaaaactt caggatggta
    33601 agaaaaagaa aatccggtat gcaaagcaaa ccggactgca aatataaact aaccatgtac
    33661 ttattgcttc ttgccggatt cgtcgtaaac gcttaatccg gcctaatcct atgataattc
    33721 aatatatttc attagaaacc ataaacccga aaacatgagt accgggcatt attaaatttc
    33781 tgaaattaca acaactgtgc cagccggttg atatccgact gaatcgcccc ggcggtgacg
    33841 tcgcgccccg cgccaggccc acggatcacc agcgggttgt cgcgatacca gcggctttcg
    33901 atggcaaaga cgttatcgca cggcagcagt gatgccagcg gatggtcttc acgcaccgcc
    33961 tccacgccca cacgcgcttt gccgttggca tcgaaacgcg ccacgtagcg tagcaccagc
    34021 cccatttcgc gggccgcttc cagccgttgc accatctgct cgttaagttc atcgccattt
    34081 tcaaagaaat ggtcgatgct gccgccttcg caatgagcag gtaccagcga ttccacacgt
    34141 acctggtccg gttcaatgtt gtaacccgct tcacgcgcca gaattaccag cttgcgcatt
    34201 acgtctttgc cggagaggtc gtcacgcgga tcaggctcgg ttaagccctg ctgccacgcc
    34261 tgatccacca gctcggtaaa cggcacgcta ccgtcgaatt gcaggaacag ccaggagagc
    34321 gtgccggaga agatcccgct gatcgacaaa atagtatcgc cgctgtcgat cagatcgcgc
    34381 acggtgtggt tgatcggcaa gcccgcaccg acggtggcat tgtacagcca gtgacgcccg
    34441 gttttttcga aggcgtcgtg gatctggcga tatttattgc tgtcgctggc tcccgccagt
    34501 ttgttggcgc tgataacgtg gaaaccgtgg ctggcgaaat caagatactg atcagcaagc
    34561 tgctggctgg cggtaacgtc cagcaccact aaatcatcat acggatgggc gcgcatccac
    34621 aggaacaacg actcttcatc ctgctcaacc gcttcatcgt tgaagaaggc taacgcgcgg
    34681 ctggcgtcca gcccgtcata gctcaacagg ctgcggcggc tgtccaccac acctgccagc
    34741 acaaactcaa agccggtacg tgccgaaagc gtgctctgct cacgggcgaa cagttccagc
    34801 caacgggaac cgatattgcc cttaccgaac aataccaggc cgatgcgttt ttctgcgcgg
    34861 aagacggact gatgcagccc ctgaatcagg ctttcggtcg ggccggtgcg cagtactgcc
    34921 accaggctga tgccgtcatc ggactgccag gtaaattcga ccggctggcc tttcagttgc
    34981 tgccagaagc ggtggcaatg cagcgggtta cgggtgacgc ctgcaccgac catcgccacc
    35041 agcgccagcc cctgacgcag gcgcagttcg ccaggtaatc ccgcttcgtc gaggattttc
    35101 agcgcactgt cggccacttc tgaggtgtag caaaattgca gcaactggcg atcgttatgt
    35161 acgccaaccg ccagcgggcg tacctgcgcg cgtttcagga tttggtcgat ctctttatgc
    35221 gccagtttga aatcctgact ggcgggcacc tgaaactcaa tcaaacagac atcatcgtgg
    35281 ctggtgacaa tacgcgcacc agtaccggag gccagcacgc gttcaatgcg cgtggaacct
    35341 tgatccggcg tgtagctaca gcgcagttgc aggtcgattt cgctgccaga aaccggctgt
    35401 aaagtacggg cgtgaagaac gggagccgcc aggcgcgcca gttcgctggc ctcatccaga
    35461 cgcagcaacg gcagcaggca ggcatctttc actttacgcg ggtcggcact gtataccccg
    35521 gcgacgtcgc tccagatggt tacgcgagaa acacccgcca gcgcaccgat ttgtgtcgcg
    35581 gaatagtcgg aaccgttacg ccccagcagc accgtttcac cggcgttgtt gcggctgata
    35641 aatccggtca ccaccagacg tttgcccgga tgttgcacca gcagctgttg cagcaacggg
    35701 taagaaagcc cttcatcaac ctgcggttgt gcggcgcgtt cagcgcgtaa aaactcgcgg
    35761 gcatcaagcc aggccgctgg cagcccttgt tgattaagta ccgcagacat caggcgtgcc
    35821 gaccatactt ccccgtggcc caccacttcc gcatacactg cgtcgttaat accgctgtcg
    35881 agcagcgccg ccaggcgctc aaggtcgctg acaaaagcgc taatgaggct atcggcttct
    35941 tcagcgggta gcagaccgct aatcagatcg cactgatagc gacgcagcgt ttgttgaacc
    36001 tgatgcgcag agagacgatc ggtctggctt agtttcaacc agttaatcaa ctggttagtg
    36061 gtgctaccgg cagcggaaac caccatcata tcgtcaggct gagagtactc cgccataatg
    36121 cccgcgacac gcaaataaca cttcacatca gccagactac tgccaccaaa tttatgcagc
    36181 tgacgacctt tcgcccctgc ctgcgcaatc acactcattt ttaccccttg tttgcagccc
    36241 ggaagccatt ttccaggtcg gcaattaaat cttcgccatc ttcaataccg gtggagatac
    36301 gcagcagcgt ctcggagatc ccggcggcag cacgcgcttc tggtgccatg cctgcatgtg
    36361 tcatggttgc ggcgtgagag attaaacttt ccactccccc taatgattcc gccagcgtaa
    36421 acaacgacag cccgcccagg aaacgacgca gcgtctgctc atcgccatcc agttcaaaac
    36481 tcaacattgc gccaaagcct ttttgctggc gcgcggcaat ttcatgcccc tgattttccg
    36541 gcagcgacgg gtgatacagt tttttcacca acggctgggt ttgcagatat ttcacaatcg
    36601 cctgcgcgtt gcgctgggcc aactccatgc gcggcaccag cgttcgcaac ccgcgtagca
    36661 gcagatagct gtcaaacgcg ccgcccgtca cgccaatatt gtttgcccac caggcaagtt
    36721 cagtgacaac gtccgggtct ttagcaatca ccacgccagc caccacgtca gagtgaccgt
    36781 tcagatattt cgtgcatgaa tgcaacacca gatcggcacc taatgccagc ggattttgta
    36841 atgccgggct taagaaggtg ttatccacca cgctcaccgc cccgacttcc ctagccagat
    36901 ggcagatttt cgcaatatcc acgacgcgta acaatggatt acttgggctt tctaccagta
    36961 ccagtttggg tttttctgcc agcgctgccc gtaatacctg ttcatcgcct tgatcaacaa
    37021 acaacacgcg atagcaaccg cgtttcgcca ggctgtcgaa caggcgatag ctaccgccgt
    37081 agcagtcgtg cggcgcaacc agcagatcgc ctggtttcaa aaagacggtc gttaccaggt
    37141 gaatcgcgga catgccggta ttagtaagta ctgcaccagc accgccttcc agttctgcca
    37201 gcgcacgctg aaccacatcg cgcgttgggt tgccgcgacg cgagtaatca tgcgcgcgcg
    37261 gttcattaaa tccggtaaag ttataggtac tggaaagatg gatcggtggg acaacgcaac
    37321 catactgttc gtcgtcattt aacccgctac gcactgcgat ggtggcctgt ttacgcgtca
    37381 tgtgatgaag ttccctgggc ttggtcggtg aaatgtcagg caccagagta aacattgtgt
    37441 taatggacgt caatacatct ggacatctaa acttctttgc gtatagattg agcaaatccc
    37501 aaatagccgt taaaattata tgcattatca cgccgacagg tgcattacac gatgtcacgg
    37561 taacgcctgt acggtaaact atgcgggttt acggtcagta cccacataaa ttgtgtggtc
    37621 tggtctcaat ttattgacga agaggattaa gtatctcatg gctgaatgga gcggcgaata
    37681 tatcagccca tacgctgagc acggcaagaa gagtgaacaa gtcaaaaaga ttacggtttc
    37741 cattcctctt aaggtgttaa aaatcctcac cgatgaacgc acgcgtcgtc aggtgaacaa
    37801 cctgcgtcac gctaccaaca gcgagctgct gtgcgaagcg tttctgcatg cctttaccgg
    37861 gcaacctctg ccggatgatg ccgatctgcg taaagagcgc agcgacgaaa tcccggaagc
    37921 ggcaaaagag atcatgcgtg agatggggat taacccggag acgtgggaat actaaagcaa
    37981 aaaagagcgg cgcggagtgg aatcgcctga tgcgctacgc ttatcaggcc tacgtcatat
    38041 tgcaatttat tgaatttgca cgaacttgta ggccggataa ggcgttcacg ccgcatccgg
    38101 cataaacaac gagcacgttg tctgcgaccc accgcttttt atacatggac gtttaactat
    38161 gaaaaacagg ctgctgatcc tcagcctgct ggtttctgta cctgcctttg cctggcagcc
    38221 gcaaaccggc gacatcatct ttcagatctc tcgctcatcg caaagtaaag cgatccaact
    38281 ggcgacccat tccgattaca gccacaccgg tatgctggtg atacgcaaca aaaagcccta
    38341 cgtttttgaa gcggtcggcc cggtaaaata caccccgctc aagcagtgga tcgcccatgg
    38401 tgaaaagggt aagtacgttg ttcgccgcgt tgaaggcgga ctgagtgttg aacaacagca
    38461 aaaactggcg caaacggcaa aacgttatct cggtaaaccg tatgatttca gcttctcatg
    38521 gagcgacgat cgccagtact gttcggaagt ggtgtggaag gtttaccaga acgcgctggg
    38581 aatgcgcgtg ggcgagcagc agaagttgaa agagttcgat ctcagcaacc cgctggtcca
    38641 ggcgaagctc aaagaacgct acggcaaaaa tattccgctt gaggaaacgg tagtttcacc
    38701 acaagccgtt ttcgacgcgc cacaactcac cacggttgcc aaagagtggc cgctgttttc
    38761 gtggtaatgg atgatgaaaa aaataattcc cgccgtagtc tgtttgttgc tttgtagttg
    38821 cacgaccttt ccgcaagctg tcgctccggt aaacggtgat gcctccaatt agttgaatta
    38881 aggatataat actcgctttt aaggttgttt catggtcaca gttaaccttc attgcccccg
    38941 ttgccagtcc gttcaggttt accgtcacgg gcagaaccca aaaagacatg atcgattccg
    39001 cggccgtgac tgtcaccggg tatttcagct cagttactgt tacgagcccc gtaaaccggg
    39061 agttaaagac caaatcgctg aaatgacttt caacggtgcc ggggttcgcg atactgcccg
    39121 tacgctgaaa atcggcatta atacagtcat ccgaacttta aaaaactctc gccacgaaga
    39181 ataacgtcat ccccggtcgc tcatgctgat gtggcactca tctgcaaact tagtcagcaa
    39241 tggagctttg tcggcagtaa agcgcgacag cactggctct ggtacgtgta caacacgaaa
    39301 actggtggtg ttctggccta cacttttggc ccccgggctg acgaaacctg tcgtgaattg
    39361 cgggcgctgc ttaccctttt accatcggca tgttgaccac tgacgaatgg ggtagttacg
    39421 ctagagagtt accgaaggag aagcatctga ctggtaagat ttttactcag cacattgagc
    39481 gtaacaatct gaccctgaga acccgaatca aacgtctggc ccgcagaacc atctgtttct
    39541 cgcgttctgt agaacttcat gaaaaagtca tcggtgcttt tatcgaaaat atatgttcta
    39601 ctaattggag tcatcaccct tctgataagc tttccttgga tgattaccag actgaataca
    39661 cagccaaaga tttttacctc attctgctct tttttctcaa atgacatata ttatatttta
    39721 catcatattc ataggcatat gacattcccc cgccc
//
LOCUS       DAOFQD010000028        39688 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.028, whole genome shotgun sequence.
ACCESSION   DAOFQD010000028 DAOFQD010000000
VERSION     DAOFQD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.028"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            198..1337
                     /gene="arnB"
                     /locus_tag="QBF46_001572"
     CDS             198..1337
                     /gene="arnB"
                     /locus_tag="QBF46_001572"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDQ6673766.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIAMTQLAKLEYLNTRRREIALQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1341..2309
                     /gene="arnC"
                     /locus_tag="QBF46_001573"
     CDS             1341..2309
                     /gene="arnC"
                     /locus_tag="QBF46_001573"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDQ6673767.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2309..4291
                     /gene="arnA"
                     /locus_tag="QBF46_001574"
     CDS             2309..4291
                     /gene="arnA"
                     /locus_tag="QBF46_001574"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDQ6673768.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPDVIFSFYYRHLIYDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4288..5178
                     /locus_tag="QBF46_001575"
     CDS             4288..5178
                     /locus_tag="QBF46_001575"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDQ6673769.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5178..6830
                     /gene="arnT"
                     /locus_tag="QBF46_001576"
     CDS             5178..6830
                     /gene="arnT"
                     /locus_tag="QBF46_001576"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDQ6673770.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6827..7162
                     /gene="arnE"
                     /locus_tag="QBF46_001577"
     CDS             6827..7162
                     /gene="arnE"
                     /locus_tag="QBF46_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDQ6673771.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7162..7548
                     /gene="arnF"
                     /locus_tag="QBF46_001578"
     CDS             7162..7548
                     /gene="arnF"
                     /locus_tag="QBF46_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDQ6673772.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAVLLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7542..7808)
                     /gene="pmrD"
                     /locus_tag="QBF46_001579"
     CDS             complement(7542..7808)
                     /gene="pmrD"
                     /locus_tag="QBF46_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDQ6673773.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7918..9273)
                     /gene="menE"
                     /locus_tag="QBF46_001580"
     CDS             complement(7918..9273)
                     /gene="menE"
                     /locus_tag="QBF46_001580"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDQ6673774.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGAEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9270..10232)
                     /gene="menC"
                     /locus_tag="QBF46_001581"
     CDS             complement(9270..10232)
                     /gene="menC"
                     /locus_tag="QBF46_001581"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDQ6673775.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMTSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10232..11089)
                     /gene="menB"
                     /locus_tag="QBF46_001582"
     CDS             complement(10232..11089)
                     /gene="menB"
                     /locus_tag="QBF46_001582"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDQ6673776.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHIMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11104..11862)
                     /gene="menH"
                     /locus_tag="QBF46_001583"
     CDS             complement(11104..11862)
                     /gene="menH"
                     /locus_tag="QBF46_001583"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDQ6673777.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLTG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11859..13529)
                     /gene="menD"
                     /locus_tag="QBF46_001584"
     CDS             complement(11859..13529)
                     /gene="menD"
                     /locus_tag="QBF46_001584"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDQ6673778.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13618..14913)
                     /gene="menF"
                     /locus_tag="QBF46_001585"
     CDS             complement(13618..14913)
                     /gene="menF"
                     /locus_tag="QBF46_001585"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDQ6673779.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLNFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14992..15297)
                     /gene="elaB"
                     /locus_tag="QBF46_001586"
     CDS             complement(14992..15297)
                     /gene="elaB"
                     /locus_tag="QBF46_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDQ6673780.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15352..15813)
                     /locus_tag="QBF46_001587"
     CDS             complement(15352..15813)
                     /locus_tag="QBF46_001587"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6673781.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15878..16795
                     /gene="rbn"
                     /locus_tag="QBF46_001588"
     CDS             15878..16795
                     /gene="rbn"
                     /locus_tag="QBF46_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDQ6673782.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16964..18202
                     /gene="elaD"
                     /locus_tag="QBF46_001589"
     CDS             16964..18202
                     /gene="elaD"
                     /locus_tag="QBF46_001589"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDQ6673783.1"
                     /translation="MQLKGAHDGYSCQQLLSIISNPTQSDICRKIYCDRGITAVFKKT
                     ALSGDEESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNL
                     AHTATNSHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENN
                     ELSDGTNFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNE
                     NIKSDPSWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKEN
                     SLKRVINSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVI
                     EQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASY
                     TQLEIKHSQPKNRASGKRVS"
     gene            18614..19220
                     /locus_tag="QBF46_001590"
                     /pseudo
     CDS             18614..19220
                     /locus_tag="QBF46_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19323..19826
                     /locus_tag="QBF46_001591"
     CDS             19323..19826
                     /locus_tag="QBF46_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDQ6673784.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(19893..21350)
                     /gene="nuoN"
                     /locus_tag="QBF46_001592"
     CDS             complement(19893..21350)
                     /gene="nuoN"
                     /locus_tag="QBF46_001592"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDQ6673785.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(21357..22886)
                     /gene="nuoM"
                     /locus_tag="QBF46_001593"
     CDS             complement(21357..22886)
                     /gene="nuoM"
                     /locus_tag="QBF46_001593"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDQ6673786.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(23050..24891)
                     /gene="nuoL"
                     /locus_tag="QBF46_001594"
     CDS             complement(23050..24891)
                     /gene="nuoL"
                     /locus_tag="QBF46_001594"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDQ6673787.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLSGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(24888..25190)
                     /gene="nuoK"
                     /locus_tag="QBF46_001595"
     CDS             complement(24888..25190)
                     /gene="nuoK"
                     /locus_tag="QBF46_001595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDQ6673788.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(25187..25741)
                     /gene="nuoJ"
                     /locus_tag="QBF46_001596"
     CDS             complement(25187..25741)
                     /gene="nuoJ"
                     /locus_tag="QBF46_001596"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDQ6673789.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(25753..26295)
                     /gene="nuoI"
                     /locus_tag="QBF46_001597"
     CDS             complement(25753..26295)
                     /gene="nuoI"
                     /locus_tag="QBF46_001597"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDQ6673790.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(26310..27287)
                     /gene="nuoH"
                     /locus_tag="QBF46_001598"
     CDS             complement(26310..27287)
                     /gene="nuoH"
                     /locus_tag="QBF46_001598"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDQ6673791.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(27284..30010)
                     /gene="nuoG"
                     /locus_tag="QBF46_001599"
     CDS             complement(27284..30010)
                     /gene="nuoG"
                     /locus_tag="QBF46_001599"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDQ6673792.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(30063..31400)
                     /gene="nuoF"
                     /locus_tag="QBF46_001600"
     CDS             complement(30063..31400)
                     /gene="nuoF"
                     /locus_tag="QBF46_001600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDQ6673793.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(31397..31897)
                     /gene="nuoE"
                     /locus_tag="QBF46_001601"
     CDS             complement(31397..31897)
                     /gene="nuoE"
                     /locus_tag="QBF46_001601"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDQ6673794.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(31900..33702)
                     /gene="nuoC"
                     /locus_tag="QBF46_001602"
     CDS             complement(31900..33702)
                     /gene="nuoC"
                     /locus_tag="QBF46_001602"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDQ6673795.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(33796..34458)
                     /gene="nuoB"
                     /locus_tag="QBF46_001603"
     CDS             complement(33796..34458)
                     /gene="nuoB"
                     /locus_tag="QBF46_001603"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDQ6673796.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(34474..34917)
                     /gene="nuoA"
                     /locus_tag="QBF46_001604"
     CDS             complement(34474..34917)
                     /gene="nuoA"
                     /locus_tag="QBF46_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDQ6673797.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLIGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(35547..36482)
                     /gene="lrhA"
                     /locus_tag="QBF46_001605"
     CDS             complement(35547..36482)
                     /gene="lrhA"
                     /locus_tag="QBF46_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDQ6673798.1"
                     /translation="MSANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQQ
                     MQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLTI
                     GASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSTF
                     KALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKAEIPWRLAYVA
                     STLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNELA
                     QVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            37405..38622
                     /gene="alaA"
                     /locus_tag="QBF46_001606"
     CDS             37405..38622
                     /gene="alaA"
                     /locus_tag="QBF46_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDQ6673799.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLIEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            38706..39305
                     /gene="yfbR"
                     /locus_tag="QBF46_001607"
     CDS             38706..39305
                     /gene="yfbR"
                     /locus_tag="QBF46_001607"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDQ6673800.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEIFVPSFHLSLDEISQDSPL"
     gene            complement(39364..>39688)
                     /locus_tag="QBF46_001608"
     CDS             complement(39364..>39688)
                     /locus_tag="QBF46_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDQ6673801.1"
                     /translation="GPHMMLGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSP
                     YPFAMVVAMAASAAFMTPVSSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIP
                     MLFPF"
BASE COUNT         9864 a        10691 c         9923 g         9210 t
ORIGIN      
        1 aaatgcctca aaattttgcc aaatgcaaag cctaaataag aaaaaatata aaaatttcaa
       61 tatttacgtc taatattagt ttcttaaggt taagttaata ttctatcctt aaaatttcgc
      121 tccaaatggc aaaatataca caacactctt tatagcaaat ataagtggac aggtattcaa
      181 tggcggaagg aaaagcaatg tcagaatttt tgcctttttc gcgaccagca atgggcgtgg
      241 aggaactcgc tgcagttaaa gaggttctcg aatccggttg gatcacaacc ggcccgaaga
      301 atcaggcgct tgagcaagcc ttttgccagt tgacgggaaa tcagcatgcc atcgcggtca
      361 gttcagccac cgccggaatg catatcacgc taatggcgtt ggaaattggc aagggtgatg
      421 aagtgattac gccttccctg acctgggttt caactctcaa tatgatctcc ttgttgggtg
      481 caacgccggt aatggtggat gtcgaccgcg atacgctgat ggtcacgcct gaagctatcg
      541 agtcagccat tacgccacgc actaaagcca tcattccggt gcattatgcc ggtgcgccag
      601 cagatattga cgccattcgc gccattggcg aacgttacgg catcgcagtt atcgaagatg
      661 cagcccatgc cgtcggtacg tattacaaag ggcgacatat tggagcaaaa ggtaccgcta
      721 ttttttcgtt tcacgccatc aaaaatatta cctgtgcgga aggtggcctg attgttactg
      781 ataatgaaaa tcttgcccgc cagctacgga tgctgaaatt tcacggtctg ggtgtcgatg
      841 cctatgacag acaaacctgg ggccgtgcac cgcaggctga agtcttaacg ccgggctata
      901 agtacaatct gaccgatatt aacgccgcga ttgccatgac acagttagcc aaattagagt
      961 acctcaacac ccgtcggcgc gaaattgccc tgcaatatca gcaagcactg gcagctctcc
     1021 cctttcagcc attaagcctt cccgcctggc cgcacgttca cgcctggcat ctgtttatta
     1081 ttcgtgtcga tgaacaacgt tgtggtatca gtcgaaatgc gttgatggaa gcgttaaaag
     1141 aaagaggcat tggtaccggg ttacatttcc gcgccgctca cacacaaaaa tattatcgcg
     1201 agcgttttcc cacgctgtcg ttaccgaata ccgaatggaa tagcgaacgc atctgttctt
     1261 tgccgctgtt cccggatatg accaccgccg atgccgaccg tgtcatcaca gcccttcagc
     1321 aactcgcagg acaataagcc atgtttgaaa tccaccctgt taagaaagtc tcggtggtta
     1381 ttcccgttta taacgagcag gaaagcttac cggaattaat caggcgcacc accacagcct
     1441 gtgaatcgtt ggggaaagag tatgagatcc tgctgattga tgacggcagt agcgataatt
     1501 ccgcgcatat gctggtcgaa gcctcacaag cggagaacag ccatattgtg tctattttgc
     1561 ttaaccgcaa ttacgggcaa cattcagcga ttatggcggg attcagtcac gttactggcg
     1621 acttaattat tacccttgat gccgatctcc agaatccgcc agaagaaatc ccccgcctgg
     1681 tggcaaaagc cgatgaaggt tacgacgtgg tagggactgt acgccagaac cgccaggaca
     1741 gctggtttcg taaaaccgct tcgaagatga ttaaccggct tattcagcgc accactggca
     1801 aagcgatggg tgattacggt tgtatgctgc gcgcctatcg ccgtcatatt gtcgatgcga
     1861 tgttgcactg ccatgaacgc agcaccttta tcccgattct ggcgaatatc ttcgcccgcc
     1921 gtgccattga aattccagta catcatgccg agcgtgagtt tggtgaatcc aaatacagtt
     1981 ttatgcgcct gattaatttg atgtacgacc tggtgacctg ccttaccaca acgccgctac
     2041 gtatgctgag tctgctcggc agcattattg cgattggagg ttttagcatt gcggtgctgc
     2101 tggtgatttt acgcctgacc ttcggaccac aatgggcggc agaaggcgtc tttatgctat
     2161 ttgccgtgct gtttactttt attggcgctc agtttatcgg catgggatta ctcggtgaat
     2221 atatcggcag gatctacacc gatgtccgcg cccgcccccg ctattttgtt cagcaagtta
     2281 tccgtccatc cagcaaggaa aatgaataat gaaaaccgtc gtttttgcct accacgatat
     2341 gggatgcctc ggtattgaag ccctgctggc tgccggttac gaaattagcg ccatttttac
     2401 ccatactgat aatcccggtg aaaaagcctt ttatggttcg gtggctcgtc tggcggcgga
     2461 aagaggcatt ccggtttatg cgccggataa cgttaatcat ccgctgtggg tggaacgcat
     2521 tgcccaactg tcgccagatg tgattttctc tttttattat cgccatctta tttacgacga
     2581 aattttgcag ctcgctcccg caggtgcatt taatctgcat ggttcgctgt taccaaaata
     2641 tcgtggtcgc gcgccgctga actgggtgct ggtcaacggt gaaacggaaa ctggcgttac
     2701 attgcaccga atggtgaaac gtgccgatgc cggggccatt gtggcgcaac tgcgcattgc
     2761 cattgcgcca gacgatatcg ctattacgct gcatcataaa ttgtgccatg ccgcgcgcca
     2821 gctactggaa cagacattac ccgccattaa acacggtaat attctggaaa tcgcccagcg
     2881 cgaaaacgaa gccacctgtt ttggtcgcag aacgccggat gacagtttcc ttgaatggca
     2941 taaaccggca tccgtactgc acaacatggt acgtgccgtt gccgatccgt ggccgggtgc
     3001 cttcagctat gttggcaatc agaaattcac cgtctggtcg tcgcgtgttc atcctcatgc
     3061 cagcaaagca cagccgggga gcgtgatttc tgttgcgcca ctgctgattg cctgtggcga
     3121 tggcgcgctg gaaatcgtca ccggacaggc gggcgacggc attactatgc agggctcgca
     3181 attagcgcag acgctgggcc tggtgcaagg ttcacgcttg aatagccagc ctgcctgcac
     3241 cgcccgacgc cgtacccggg tactcatcct cggggtgaat ggctttattg gcaaccatct
     3301 gacagaacgc ctgctgcgcg aagatcatta tgaagtttac ggtctggata ttggcagcga
     3361 tgcgataagc cgttttctga atcatccgca ttttcacttt gttgaaggcg atatcagtat
     3421 tcattccgaa tggattgagt atcatgtcaa aaaatgtgat gtcgtcttgc cgctggtggc
     3481 gatagccacg ccgattgaat atacccgcaa cccgctgcgc gtatttgaac tcgattttga
     3541 agagaatctg cgcattatcc gctactgcgt gaagtaccgt aagcgaatca tcttcccgtc
     3601 aacttcagaa gtttatggga tgtgtagcga taaatacttc gatgaggacc attctaattt
     3661 aatcgtcggc ccggtgaata aaccacgctg gatttattcg gtatcaaaac aattacttga
     3721 tcgggtgatc tgggcctatg gcgaaaaaga gggtttacag ttcaccctct tccgcccgtt
     3781 taactggatg ggaccacgac tggataacct taatgcagcg cgaattggca gctcccgcgc
     3841 tattacgcaa ctcattctca atctggtaga aggttcaccg attaagctga ttgatggcgg
     3901 aaaacaaaaa cgctgcttta ctgatattcg cgatggtatc gaggcgttat accgcattat
     3961 cgaaaatgcg ggaaatcgct gcgacggtga aattatcaac attggcaatc ctgagaacga
     4021 agcgagcatt gaggaactgg gcgagatgct gctggcgagc ttcgaaaaac atccgctgcg
     4081 ccatcatttc ccaccgtttg cgggctttcg cgttgtcgaa agtagcagct actacggcaa
     4141 aggatatcag gacgtagagc atcgtaaacc gagcatccgc aatgcccacc gctgcctgga
     4201 ctgggagccg aaaattgata tgcaggaaac catcgacgaa acgctggatt tcttcctgcg
     4261 caccgttgat cttacggata aaccatcatg accaaagtag gcttacgcat tgatgtcgat
     4321 acctttcgtg gcacccgtga aggcgtgccg cgtctgctgg aaatcttgag taagcataat
     4381 attcaggcca gcattttttt cagcgtcggc ccggacaata tgggccgcca tctctggcga
     4441 ctggtgaagc cacagttttt gtggaagatg ctgcgctcaa acgcggcatc gctttatggc
     4501 tgggatattt tactggcagg tacggcctgg ccaggtaaag agattggtca tgccaatgcc
     4561 gatatcattc gtgaagcggc taaacatcac gaagtcggcc tgcacgcctg ggatcaccat
     4621 gcctggcaag cccgtagcgg taactgggat cggcaaacaa tgatcgacga tattgcacgc
     4681 ggtcttcgca ctctggaaga gattatcggt caaccggtaa cctgttctgc cgctgcgggc
     4741 tggcgtgccg accagaaggt gatcgaagca aaagaagcgt tccatttgcg ctacaacagc
     4801 gattgtcgtg gggccatgcc gttccgtcca ttgctcgaat caggaaaccc tggcactgcg
     4861 caaattccgg tgaccttacc cacctgggat gaagtgattg gtcgggatgt gaaagcagaa
     4921 gattttaacg gttggttact caaccgcatc ctgcgagata aaggcacgcc ggtttatacc
     4981 attcatgcag aagttgaagg ctgcgcttat cagcataatt ttgtggatct cctcaaacgc
     5041 gcagctcagg aaggcgtgac attttgccct ttaagcgaac tgttatcaga gacgttgccg
     5101 ctcggacaag ttgttcgcgg aaatattgcc ggacgtgaag gctggctggg ttgccaacaa
     5161 attgcgggta gtcgctgatg aaatcggtac gttaccttat cggcctcttc gcgtttattg
     5221 cctgctatta cctgttaccg atcagcacgc gtctgctctg gcaacccgat gaaacgcgtt
     5281 atgcggaaat cagtcgagaa atgctggcat ccggcgactg gattgtgccc catctgttag
     5341 ggctacgtta tttcgaaaaa cccattgccg gatactggat taacagcatt gggcaatggc
     5401 tatttggcgc gaataacttt ggtgtgcggg caggcgttat ctttgcgacc ctgttaactg
     5461 ccgcgctggt gacctggttt actctgcgct tatggcgcga taaacgtctg gctctactcg
     5521 ccacagtaat ttatctctca ttgtttattg tctatgccat cggcacttat gccgtgctcg
     5581 atccgtttat tgcattctgg ctggtggcgg gaatgtgcag cttctggctg gcaatgcagg
     5641 cacagacgtg gaaaggcaaa agcgcaggat ttttactgct gggaatcacc tgcggcatgg
     5701 gggtgatgac caaaggtttt ctcgcccttg ccgtgccggt attaagcgtg ctgccatggg
     5761 tagcgacgca aaaacgctgg aaagatctct ttatttacgg ctggctggcg gttatcagtt
     5821 gcgtactgac ggttctccct tggggactgg cgatagcgca gcgggagcct aacttctggc
     5881 actatttttt ctgggttgag catattcaac gctttgcact ggatgatgcc caacatagag
     5941 ctccgttctg gtactacgtg ccggtcatca ttgccggtag cctgccgtgg ctgggattac
     6001 tccccggtgc actgtacaca ggctggaaaa accgcaagca ttccgcaacc gtctatttgt
     6061 tgagctggac gataatgccg ctgctgtttt tctccgtcgc taaaggtaaa ttgcccacct
     6121 atattctttc ctgctttgca tctctggcaa tgctgatggc gcattacgct ttgctggcag
     6181 caaaaaataa tcctctggcg ctgcggatta atggctggat taacatcgct tttggcgtca
     6241 ctggcattat tgccacattt gtggtctccc cgtggggacc aatgaacacg ccggtgtggc
     6301 aaaccttcga gagctataaa gtcttttgtg cctggtcgat tttttcgcta tgggcatttt
     6361 tcggctggta caccttaaca aacgtcgaaa agacctggcc ttttgccgcg ctttgcccgc
     6421 tggggctggc gttgctggta ggattttcaa ttcctgacag agttatggaa ggaaaacatc
     6481 cgcaattttt tgtcgagatg acacaagaat cacttcagcc aagccgctat attcttactg
     6541 atagcgtcgg tgttgccgca ggtctggcat ggagcctgca acgcgatgac atcatcatgt
     6601 atcgccagac aggtgagttg aaatacggcc ttaattatcc ggatgcgaaa gggagatttg
     6661 tcagcggtga tgagttcgca aactggctta atcaacatcg tcaggagggg attattactc
     6721 tcgtgctttc ggttgaccgc gatgaagata tcaacagtct cgccattccg cccgcagatg
     6781 ccatcgatcg tcaggagcgt ctggtgctga ttcagtatcg tcccaaatga tctggctaac
     6841 attagtcttt gccagcttgc ttagcgttgc cgggcagttg tgtcagaaac aggcaacctg
     6901 ctttgtggcg ataaacaaac ggcgcaaaca tatcgtgctg tggctgggac tggcgctggc
     6961 ttgtcttggt cttgccatgg tgctctggct gctggtcttg cagaacgtac cggtaggcat
     7021 tgcctacccg atgttaagtc tgaattttgt ctgggtgacg ctggctgcag taaaactgtg
     7081 gcacgaaccg gtatcgccgc gtcactggtg tggggtggcg ttcattattg gtggcattgt
     7141 gatcctcggg agtacggtgt aatgggcctg atatggggat tattcagcgt cattattgcc
     7201 tcagttgcgc aattaagcct gggttttgcg gcaagtcatc tgccgccgat gacgcactta
     7261 tgggatttta tcgcggttct gttagctttt ggcctggatg cccggatact gttactcggg
     7321 ctgctgggtt atctgctttc cgttttttgt tggtataaaa cgcttcataa acttgcgcta
     7381 agcaaagcct atgccttgct cagtatgagt tatgtgctgg tatggattgc ttcaatggta
     7441 cttcccggct gggaagggac tttttcgctg aaagcactac tgggagtagc ctgtattatg
     7501 agcgggttga tgctgatttt cctgcccatg acaaaacaac gttactgagt tttccctgcc
     7561 actttacact gccgttccca ctcatcgggg ctataactgc ttgccgagag gactttcacc
     7621 gtatgcagct tttcacgatt aatacaataa agcgcattct gcaaaggcga gagtaaatcc
     7681 cccactttca cggcgaagtc actcttcact tcggcgatca tttttatcgc gccgccagca
     7741 tcgcagagca taagtacatg tctgttatct tgtttattgc agcacgattt tttgaccagc
     7801 cattccattg cattatcctg tttgctaaga gttttcacat caatttacag attaacagat
     7861 aaactcttta tcaacggcaa tggaatggca gaaatgagaa gtgaaacgga gggttaatta
     7921 ttgctgacgt tgcacccact cttttagcgc ctgacgtgaa attttaatac cgccgttttt
     7981 cagttcaggc ggcaacgtta accaacgcac cggttgctga aaacgggcca gcttatcttt
     8041 cacccattca ctaagatcaa cgctctcgtg gtcatactcc atcaccgcca ccggtcgatg
     8101 accaaactcc ttgtcggcaa cggggacgat aaacacctgc aaaaccgcag gatgtgcagc
     8161 aattacgcgc tcgacttctt ccggctgaat accctctgcg ccactgaaga atagattgtc
     8221 taaacgtccg acaatggtca gcttgccatt atgcatctca ccgcgatcgc gcgtagcgta
     8281 ccagccttcg tcattaacca gtgaaaccag ttgcccgtta cgccagtaac cttctgccat
     8341 actggcagcc cgcagccaca cttcattatt aacgattttc acttcccgac ccggcagcgg
     8401 cgaaccaacg tctgccaggc cgtcggcttc tttcgcacac accgtggagg caaactcggt
     8461 cagaccatag ccgcaaaagc aacgaatccc ctgctcgcgc gcctgttccg tcaactcgac
     8521 cgggatagcc gcgccgccaa gtaacaccgc tttcagggaa acggaactgc ggttaaccag
     8581 caaacgccag agttgtgttg gcaccagtga agcatgagta cagcctgcca gcatttgctc
     8641 caatggctgt ttatcacgca ccgtcatccg cgcaccagcg tataaccagc gccacataat
     8701 tccctgaccg gagacgtgaa acagcggtaa agagagcaac cagtcatcgt gatcgccaaa
     8761 cggaatcaac gataacaccc cttcggcact ggcaagatgg gcctgataag tatggacagc
     8821 ggctttcggc aacccggtag aacccgaggt caacgtcatt gagcacagac gcgtcggctg
     8881 ccacgtagcg gcatgtgcgc cttcaacccg ctgaatgtgc agcgacgtta acgccggaaa
     8941 cgtgttttcc ccatccggca ccagcgcaaa ttgcagcgtc agattgggca gcaattcttc
     9001 aagcaacggt tgcggcagct gagggttcac gggcaacacc cgcgccccgc attgcagtaa
     9061 cgccagccag gcgagcagcg tttgcggcgt attccacgcc cgcaacatca cgccgctgcc
     9121 ctcaacaacg ccctgcgccg caaagcccgt agctaactca tcgacgcgag cacaaagctc
     9181 gcgccagtta agttgctcgt cattaagacg taaggcgatg gcttctcccc gcacttgccg
     9241 ccagtgacgc cacggccagt cagagaagat cataacaacc gctccagtgc atcaacttcc
     9301 acgacaggca gcgtgctacc cggccagcga cgtacctgct gcgcctgcat cagatccagc
     9361 gtgtccagcc ctggaatggt gtccggcgtt aaccaggcgg caatccgcgc cagttgcgtt
     9421 aagcctaagc tcgattcaat ggaagaactg atcaccgccg tcagccccag cgcgtgcgcc
     9481 gcctgtacct gctcgcgtac tttctccaga ctgcccgtga gcgtgggttt gataactacc
     9541 gcgcgcacgc cctcttcagc cacaaaggca aaatccggct cgcgcaagct ttcatcccag
     9601 gcaatggcaa tgccggtttc acgggcaaac gctcgcgaat catcgcgggt tttgcacggc
     9661 tcttcgagaa acgcgatgcg gtggcgataa tccgggttaa catatttggc aaactgctgg
     9721 cctttcagcg gtgtccaggc gcgatttgcg tcaagacgca aatgcagatc cggaattgcc
     9781 tccagcaaca gattaaccac catgccgtcg cgcacggctt cgtacaatcc cactttgacc
     9841 ttcgccactt tctcgcctgg catatcagca agtttgagga tcagatcgtc aggatcgcca
     9901 ttacacagtg gtgccgcacg gtaattggcc gcctgcggga gcgtatctgc cagctccgcc
     9961 aatgcacagc ttacgccaaa ggcaacggaa gtcatctgcg gtagctcgca atcgcctgcc
    10021 agccagttat ttacccaggc aagcagcacg ctttgcgcct cttcccaggt ttcctggctg
    10081 aagcccggca gtggggagat ctccccccac ccttcgcgct cgccctcacg caggcaaacg
    10141 tacagcccgt cgcgggtttt taaccgcctg tcgcgcagaa ccacccccgc gtccatgggg
    10201 atctgccagc ggtatacctg cgcgctacgc attacggatt ccgtttaaat ttgctgaaat
    10261 caggctgacg tttctggttg aaagcgttgc gaccttcctg accttcttcc gtcatgtaga
    10321 acagcatggt ggcgttgccc gccagctcct gcagccccgc ctgcccgtca cagtcggcgt
    10381 tcagcgcagc tttcaggcag cgcagcgcca tcgggctgtt ttgcagcatt tcgcggcacc
    10441 aacggacggt ttctttttcc agatccgcca gcggtaccac ggtgttgaca aggcccatat
    10501 ccagcgcctg ttttgcgtcg tactgacggc acaggaacca gatttctcgc gcttttttct
    10561 gcccgacgat acgagccatg taggaagcgc cccagccgcc gtcgaaggaa ccgactttcg
    10621 ggccagtctg accgaagatg gcattatctg ctgcgatagt caggtcgcac ataatgtgca
    10681 gaacgtgacc accgccgatg gagtagccag ccaccatcgc gacaaccggt ttcggacagg
    10741 tacggatctg acgctggaag tccagcacgt tcaagtgatg tacgccggaa tcatctttat
    10801 agccgccgta atcaccacgc actttctggt caccaccgga gcagaacgct ttatcgcctg
    10861 cgccagtcag aatgatcacg ccgatattgt cgtcataacg cgcatctgcc agtgcctgga
    10921 tcatctcttt taccgtcaga ggacggaagg cattgcgcac ctgcggacga ttaatggtga
    10981 tttttgcgat accgtcggtg gatttttcat aacgaatgtc ctcgaaacct tcggagcagt
    11041 cgtgccattc aaccggtgcg taaagcattg cttcatcagg ataaatcata ttgtgtcctt
    11101 tagtcagaaa cgcaagatct gcgccagact tgcgattacg ccagcgggat tttcccgatg
    11161 cgcgttatgt ccggcgcgag gaatgacatg gcagtcggca gccagttccg ccgccagggc
    11221 gcggaatttg ctgtcacgtt caccacataa ataataaaac gcaaatgtgc gggcgctaag
    11281 gttagcacgt aaatcaggct ggacggcgag agaagtcgcc tccagcatgg cggcaagggt
    11341 tgcgccatta ttgttgctgc gcagcaccac cagctcccgg cgttgatcgt cattgagcga
    11401 ggcaaaaaca ggctgttgat accagtcggc aaataccgcc gttaacggtt ctgtgcaaaa
    11461 acgctgcgcc cattggcgat cggaacgctg acgttccgca cgttgttcag cattttgcag
    11521 ccccggatgc ccgccttcga caataacccc acaaagcccc gtcagcccct ggcaagccgc
    11581 catcatcgcc acccgtccgc caagcgagta ccccaccagc cagaagtcaa ggatgttgta
    11641 actaaccaag gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc
    11701 cgccgaacca ccatgacctg ggagatcaac atacaaccgt gagtagtcgg caaacgcctc
    11761 gcccacttct tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg
    11821 taaacctggt tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc
    11881 agaagttgct ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg
    11941 gtggttggcg tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga
    12001 cgatgatatt tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc
    12061 agatagaaac gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg
    12121 ttgttgttca ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg
    12181 ttgagatcgt aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc
    12241 cgctgaacgc ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg
    12301 tacaccgggt aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg
    12361 ttaccaacaa acaattgccc ctgttcaggc agatagtcgc agatgcgatg cgccagttgc
    12421 gcttcgccaa acgcatcacg gcgggcaata accgcctgca ttgcctgttc cgccaggcgc
    12481 gggatttcaa cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca
    12541 atattggcaa ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca
    12601 tcaacaatcc agtactcttc tggttcacag cttgcctgcc attgcaggag ccgtttcccc
    12661 gtcaggctgc ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg
    12721 gcattgccta accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg
    12781 ccaatcagcg gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca
    12841 ctcatgcgcc cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt
    12901 ttttcacttt ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca
    12961 cccagacgct gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca
    13021 aacgggcagt tgatatggac tccccccgca tgaagcgtac cgagagcgtg gtcgatggta
    13081 gaaaccagcc aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat actgtgcgtg
    13141 gggtgagagg cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc
    13201 tccggcgggc gatcggcggt taagagaatc agtttttctc cggttaaccc ggcttcaatc
    13261 agtgccggat agagatttgc caccgccgtg ccggaggtca caatcaccgc caccggctgc
    13321 ttgctgactt tcgccagccc cagcgccaga tgccccaacc cacgctcatc gaaatgggtg
    13381 tggtgaatga atgcggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcct
    13441 ggggcgatac agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc
    13501 cagcgtcggt taaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag
    13561 tataaggagc tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta
    13621 ttccatttgt aataaagtac gcagccctgc cgctttgttg tcgatttcct gccactcttg
    13681 ctcggggtcg gaaccacgga caatgcccgc gccagcatat aatcgcacga cattgccgct
    13741 aatttttgct gagcgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc
    13801 agaaccggcg taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag
    13861 atcgcgcggt aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac agatcacatc
    13921 atccgctttg ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg
    13981 cagtacctgc ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat
    14041 atcttccacc accagcatgt tctcgcgctg gtttttatca tccgccatca gccactctcc
    14101 taactgctgc gcctgcttat catcaggatt atttgctact gttcccgcca gcgcttcagt
    14161 acgcagcgct ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcatt
    14221 ttcgccatca aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc
    14281 catcatcgcc gccgcgttga ccggacttgc gaaattcagg tcagttgccc gagcgagcac
    14341 cactttgtcg agctcacctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca
    14401 gcccgttttg tccggccagt gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg
    14461 cttgatactc accagtgtgg cgataaattc ttttgcctga atcgcatcgt gctgaaggga
    14521 gctttcgctg aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag
    14581 gcggggtaaa agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa
    14641 gtcggcgtgt tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg
    14701 ggtaatcgcg cccaggacga cagcttcttc atcaccatta cgttgttgcc agtagaattg
    14761 cgggtatgtt tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa
    14821 aggaatatcg ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag
    14881 tagattttcc agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca
    14941 cattatacgg ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg
    15001 tgccagcaac agtcctagta ccagcccaac ggccgcgccc acaccaattc cttgccaggg
    15061 tttttcgtgg acgtagtcat cagcacgata aactgcctgc ttcgcccgat agtaataact
    15121 gtctgaagcc tggctaaccc gttttttcac atcatccagc gctttttctg cacgcgcttt
    15181 cagctcaaca tatttctgat cggcgggatc gccagaggag cggagcacct cttccagtgt
    15241 ttcactaagc agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct
    15301 cgttctccat ttgcgtaaaa cctgtcacta actatagaca agggttgttg attacgcctg
    15361 aattacctcg cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac
    15421 agggataaaa ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac
    15481 aggcttatca ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca gctgctgccc
    15541 cactttttcg ccacgcaacg cttcactgac aatcacccga cctataacga ccggctcaag
    15601 atcatcatca cttttcagaa tcctcgcata tgccaccagt tcatcatttt tccaaccgag
    15661 aatatggcga ttatcccccg tcaaatcatc accatcgata tcctgataag ggcaattctg
    15721 ctcgacaaca aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa
    15781 ttcagagtga tgcagatctt gccattcaat cataatgctc tccttttaga tgtatgcctt
    15841 atactagccg gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac
    15901 ttcggccggt gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca
    15961 tccgacccag agcggtcttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact
    16021 gcataccgcc tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga
    16081 tcatcttttt ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc
    16141 cttaacgatt tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag
    16201 cggctcatgg accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga
    16261 tgatggcctg cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta
    16321 tcgtattgaa gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg
    16381 cgtgccgcct ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca cgctggaaga
    16441 tggaaggcag attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc
    16501 tattttcggc gataccggcc cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt
    16561 catggtgcac gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca
    16621 tagctctaca cgccaggctg cgacactagc ccgtgaggct ggagtcggca agctaatcat
    16681 tacccacgtc agctcgcgct atgatgacaa aggttgtcaa cacctgttac gtgaatgcag
    16741 gtcaattttc ccggcgactg aactggcgaa tgatttcacc gtgtttaacg tttaactctg
    16801 cttattatta aaaacagggt gaaacttgcc ctgttatagc aacccgcgct acatatatcc
    16861 acccacacag gcatattgag cgatttttga tatttattca tctccttacg cactatattc
    16921 agagcaataa gttgcctctt ttcatcttga ttcagcacaa cctttgcaac taaaaggagc
    16981 ccatgatggt tacagttgtc agcaattatt gtcaattatc tcaaacccaa ctcagtcaga
    17041 catttgcaga aaaatttact gtgaccgagg aattactgca gtctttaaaa aaacagcgtt
    17101 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    17161 tggaaaaaaa gctgaagata ttctttacca tattgttaga aacccaacaa atgagaccct
    17221 atcgattatc cgacttatta aaaatgcctg tttaaaatta tataatttgg cacataccgc
    17281 aaccaactcc caccttaaac caactggtcc ggataattca gatgttctcc tgttcaaaaa
    17341 gctattctcc ccttcgaaat taatgacaat tatcggtgat gaaattcctc ttatatctga
    17401 aaaacagtcg ctttcaaagg tgcttttaaa tgatgagaat aatgaactga gtgatggtac
    17461 aaacttctgg gataaaaatc gtcaattaac cacagatgaa atagcttgct atcttcagaa
    17521 gatcgccgct aatgcaaaaa atactcaagt caattatcct actggtctct acgtccccta
    17581 ctccaccaga actcacctgg aagacgctct caatgaaaat attaagagcg atccatcatg
    17641 gccgaatgaa gtccagttat tccccataaa tactggcgga cactggatat tagtttcgct
    17701 acagaaaata gtaaataaaa aaaataataa actacaaata aaatgcgtca tattcaactc
    17761 attgcgtgca ctaggccatg ataaagaaaa ttcacttaag cgtgtcatta atagttttaa
    17821 ttctgaactc atgggagaaa tgtcgaataa taatataaaa gttcatttaa atgaaccaga
    17881 gataatattt ttacatgccg atcttcagca atacttaagc caaagttgcg gtgcgtttgt
    17941 gtgcatggca gcccaggaag tgattgaaca aagggaaagc aattctgaca gcgcccccta
    18001 tacgttatta aaaaactatg ctgacagatt taaaaaatat tcagcagaag agcagtacga
    18061 aattgatttt caacatcgac tggtaaacag aaattgttat ttagataaat atggcgatgc
    18121 acgaatcaat gcttcttata cacaattaga aataaaacac tcacaaccca aaaatagagc
    18181 atccggcaaa agagtgagtt aacatcattg ttaattaatt cgaccagtca gcaaatatgc
    18241 tgactggtcg aacatagttt gctaaacagt catcactgac tgatgtcagt cacaccatcc
    18301 gccagttcaa tcttcgggta tttccagcgt atctcggcac gcgattgctg gtctgttgat
    18361 gtagtggatt gttgttgctg acttttctta ccctcagagt tctgccccct gtcaactcaa
    18421 tctgccaata aataacataa ttatattttt ccttatatga attccttttt aaaatagcga
    18481 agccactgta aaagatactt tttgtataga tctaacaacc gtaataaaac aaacactcat
    18541 aactcattat aaataatgat aaaataacgt cttttattga taatttatat atgaaaaaaa
    18601 atccttttgt tatattattt ctgatttcac tccctctgat tatttttacc aaccctgggt
    18661 cactgcatta tcgccaacgc cccgacacgt cagtcctgag caattagaaa aaacagttcg
    18721 ttatcttaca caaactgtgc atccacgtaa tgccgacaat attgataatc tgaataggtc
    18781 cgtagagtat ataaaagagt tctttgtcag tagcggtgcc agagttaccg cgcagggcgt
    18841 ccccgattgg ctattacgac tctgcgcctg gtagccagga ttacccttac ccgcaatgtc
    18901 ctggctttat cccgatcggg gggactttat tgcctggtcg gcagaataca ggatatcaac
    18961 gccgttcgtc aggtaaaaac ggcattgtta tcatcccggg atttatctgt ttattctatg
    19021 aatgccccat ggtttattcc cggtattgat ttctctgacc acctgaatta ttggcaacac
    19081 gatattcccg ccgtaatgat tactgacacc gctttttatc gtaataaaca ataccacttg
    19141 cccggtgata cagcagacag attgaattat cagaaaatgg ctcagatggt gttataacct
    19201 tgttacacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
    19261 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
    19321 aaatgggaat gattggttat tttgcagaaa ttgactcaga aaagattaac caactgcttg
    19381 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
    19441 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg
    19501 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttgg tgaacacagc ctcgaagatg
    19561 gcatagatgg cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac
    19621 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
    19681 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga
    19741 tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc
    19801 atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat
    19861 gtcggcggct ttctgactta caaactaaca gattacatca gcggcattgc taaccgcaca
    19921 atactaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc
    19981 accacgatac cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc
    20041 ggtgcgtgca ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa
    20101 ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg
    20161 tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc
    20221 gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa
    20281 tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg
    20341 ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt
    20401 tgcagcgcaa tcagcgctac cagcagatag ccgaggtgag agatagatga gtaaccgagc
    20461 agacgtttga tattggtctg gctcagcgcc atcaggttac cgaagatgat ggaggcaaag
    20521 gcgataatcg ccagcaccac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac
    20581 agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc
    20641 ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt
    20701 ttgaagccga ggccaacaat catcaggccg aaacctgcca gcaacagcgg ctcgttgagc
    20761 ataccgtcgc caaggttttt acccaacgcg acaaacgaca ggtcgccaga ctgcgcatac
    20821 accagcgcca taccaaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg
    20881 atactggctt ccagtgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc
    20941 aaagagatca gttcgatacc gaggaacaga gacgccagat ggttggcatt cgccagcagg
    21001 atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag
    21061 ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg
    21121 gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc
    21181 tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg
    21241 ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg
    21301 acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac
    21361 ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc
    21421 gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac
    21481 aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt
    21541 ttcgctttac cgaagtaagc gcgatgtaac atcgccagcg aataaacaga tgcaaagacc
    21601 agcccaaagg tagagataac ggtaatcacc ggtacaacct ggaagctgcc gaacagaatc
    21661 ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac
    21721 agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg
    21781 cgggtatgga tacgttcata aagctgacca caaagaataa acagacccgc cgccgacaaa
    21841 ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagata
    21901 gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg
    21961 gtctgggcga aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagca
    22021 attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg cagcaaaccg
    22081 taagcggcag ttttcagcaa gatccccgcg aggtcaacgg aaccggcggt cggagcctgg
    22141 gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg
    22201 atgaagaagc ccagcattaa caggtattcc acaccactgg acattggcgt attcagcagc
    22261 tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg
    22321 atggcaatca acatcaccag gccactcgcc tgggtgtaaa tgaagaactt ggttgccgcc
    22381 gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc
    22441 ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg
    22501 ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg
    22561 atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc
    22621 agcagcgaca gcccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc
    22681 atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc
    22741 tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag
    22801 cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga
    22861 attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg
    22921 accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg
    22981 atgcaccgcg aaggcggctt atccgaccta cgttcttata tgcccagggg caatcccaca
    23041 atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat
    23101 ggatgccaca taccagcgca gatagccgtt ctcacttaac agcagacctt tacctgcaaa
    23161 gcgggaaagg acagccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca
    23221 ggcaataccc aggaacggct tgacgaacac tttgtcatac agccagtcaa atccccaggc
    23281 gttgtaccac caggtgccca gcagacggcc cggcgcactg ttggcgatgg aggtcaccag
    23341 agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc
    23401 agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac
    23461 gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat
    23521 cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg
    23581 gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc
    23641 tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact
    23701 gaagaagccc gcagtgacca gcggtagtgc cgacagtgct gcgccgccca ccaggaagca
    23761 gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg
    23821 atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg
    23881 agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta
    23941 gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc
    24001 ggcaaaaccg gacagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc
    24061 cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat
    24121 cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca
    24181 tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc
    24241 ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg
    24301 gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc
    24361 acccacacgg gtcacgacga acgctttcat tgccgctgcg ccattcttcg gatcggtgta
    24421 atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag
    24481 cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc
    24541 gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag
    24601 gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt
    24661 aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgactg
    24721 ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt accagcgccg ccaggcccac
    24781 agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag
    24841 caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc
    24901 tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca
    24961 aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg
    25021 tcggtctgac cccagtagct tccggctacc acaaaggcca gcgcggaggc gttaatcatg
    25081 atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca
    25141 agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct
    25201 ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt
    25261 cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca
    25321 ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac
    25381 cgtcgatacc ctgatcatta acaccaagga tggcgtaaac aatcaccacc agcatgatgg
    25441 ccgacaaaat tgccggacca atccacacct gcggtttcag ccactggcgt tcctgttcga
    25501 tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac
    25561 ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcccagtgag aagaacaccc
    25621 cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg
    25681 tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca
    25741 ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc
    25801 ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg
    25861 gtatttgccc ggaccggaga tcagcagatc ctctttttcg taaaccagat cctggcgctt
    25921 gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc
    25981 gcacagacca cagaaaatgc agcgtgagaa gttgatgcgg aaaaattccg ggtaccagcg
    26041 accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcgca
    26101 gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc
    26161 acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc
    26221 gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa
    26281 ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc
    26341 gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc
    26401 tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac
    26461 gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag
    26521 aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac
    26581 agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct
    26641 tccggctggt caaacgggtg acggtgacat accgccacgc ccgcgatggc aaaggtaata
    26701 aaaccaaaga attgcgggat aacgttccac acatgcgcct ggctgttgac gatgtcggtc
    26761 atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg
    26821 tagctcaggg tctgcgcaga agcacgcatc gcacccagca acgagtattt gttgttactt
    26881 gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa
    26941 atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat cgcaaaggcc
    27001 agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat
    27061 ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa
    27121 ccgccccagc caacacggtt aggtccgtaa cggttctgga acagacccag caggcgacgt
    27181 tcgccaaagc tcatgaatgc cccgcaggtg acaaccacca gcaggatcac caccgctttg
    27241 aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct
    27301 tgagatcctc aagatgcgcg ccagccagca ccggagcaat accggacata cccatcggca
    27361 agcccacctg ccctgccgtc agcccttcag cgatttcaac cggcagcgtg accgtgttgc
    27421 catcgtaact aaaggagacg tgtgtacctg cgttcacacc taacttcgcg gcatccgctg
    27481 ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca
    27541 attcatcgct gccaaacagg tggtaatacg gcgcgatacg ccatttcccg tcctgcggct
    27601 ggaagcgtgc cggtacgctg gtgaagtaat ccagaccatt ttcgctggtt tcaaacagac
    27661 gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg
    27721 cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcagtcg
    27781 gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg
    27841 gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac
    27901 gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacctg
    27961 ccagttccgg gattttcgcc acaacagcgt caatcacatg gtcgagctgc gtccagtcca
    28021 cttcacggct cagcagggtg ctgtgcagcg agtgtaacca gcgccagctt tccagcatga
    28081 cagttttgct gtcgtaataa gcaggatcgt aaacctggaa gaaacgttgg gcgcggcctt
    28141 cgttgttgat caccgtaccg tcgctttcag caaagctggc agcagaaagt accagatggg
    28201 cgttttccat aatcgctgtg cgttgatgat caaccaccat caccagcggt gctttagcca
    28261 gcgcagcatt cacgcgggta gcagaagcgt gacgatgcag atcgttttcc aacaccacca
    28321 ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca
    28381 taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgataccg acgtcagcgc
    28441 cgcgaccttt cagggctttc gcgacgttag ccgccgcctg aatcacctct aagctaccgg
    28501 cgttcgtccc ggatataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt
    28561 cgattttgct ttgcagctca ggttcgatac cgtcaaccgc tggtgcagag ttatccagcg
    28621 catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg
    28681 ccgcgatatc atccagacgg gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac
    28741 gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgccgcca
    28801 tttcgcgcgc tttacctttc acagcctgac gcactgccag cgcgacgcgc gcgccggtct
    28861 gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg
    28921 gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac
    28981 cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc
    29041 tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat
    29101 ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct
    29161 gtactggctg acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt
    29221 cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc
    29281 cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg
    29341 caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc
    29401 cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg
    29461 ggcgaccgaa gtagacgttg tcgtgcgcac cgtaaacgcc cagatctgta ccgtcagcgt
    29521 aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga
    29581 tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc
    29641 tgtgtccggt catcacagtc atatcctgaa gatggcagtt accgccctct tcacataccg
    29701 gacagtcgtg cgggtggttg gtcatcaacc actcgaccac gctttcacgg aactgtttcg
    29761 cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca
    29821 ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag
    29881 caccgacact tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca
    29941 gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa
    30001 ttgtagccat tagcatgctt ccagttttct cagtcagaga ctgagcgtta atcgaaattc
    30061 ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg
    30121 aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac
    30181 ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac
    30241 agttgctcaa gtgtttcgat atcgcccggc tgaccttcac cacgctccag cgcacgcaga
    30301 attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg
    30361 gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc
    30421 atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc
    30481 agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta
    30541 aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg
    30601 ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg
    30661 gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc
    30721 gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg
    30781 gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg
    30841 attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg
    30901 aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca
    30961 atggcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca
    31021 cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt
    31081 tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc
    31141 aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa
    31201 aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc
    31261 ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg
    31321 tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga
    31381 gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca
    31441 gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc
    31501 agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca
    31561 gctttttctc gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt
    31621 cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg
    31681 ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac
    31741 catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac
    31801 gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct
    31861 caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat
    31921 aacaaaatcg atactgccca gataaacaat caggtcagac accaggctgc cgcggatcgc
    31981 cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat
    32041 ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg
    32101 gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag
    32161 ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc
    32221 tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg
    32281 cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg
    32341 gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc
    32401 gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc
    32461 ataggcggca acgccctggg aacgaccttt cagaatggta ttttgcagcg ccgctttctc
    32521 gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcaggc gatcccagcc
    32581 gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc
    32641 cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg
    32701 cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg
    32761 gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc
    32821 cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat
    32881 acggtcggta tacggaatgt agctgtgcca ggattggcgc tcgcccattt tctccgcacc
    32941 acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa
    33001 aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa
    33061 gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc
    33121 catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc
    33181 cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag
    33241 gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt
    33301 agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac
    33361 cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc
    33421 cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa
    33481 cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc
    33541 acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg
    33601 cccaaaacgg ttgcgcagtt cgccaatcac cggatcatca agatgatcgc gggtctgcca
    33661 ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg
    33721 actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt
    33781 gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc
    33841 accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac
    33901 cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg
    33961 catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt
    34021 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcacc
    34081 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
    34141 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctgacgcg gcgaagcacg
    34201 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
    34261 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
    34321 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
    34381 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
    34441 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
    34501 cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
    34561 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
    34621 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
    34681 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
    34741 ggagccgacc gagtcgatac cggattcaaa cggcacgttt tttgacctcg cgcgtgcgcg
    34801 accgcctaaa aaccaaccgc ctatcagcat caggcaacac aggccaatgg caacgataag
    34861 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
    34921 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
    34981 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
    35041 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
    35101 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
    35161 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    35221 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    35281 gatctgtgac acgcttttgt cattccataa caaaaacgca gcaacaaatt tacgtatttt
    35341 ttaacatcat tgtagcaggt gatttttttc aggctattat ttgtgcgttc gggacgtgaa
    35401 tctctggtga ttgaaaaatg aacagttttg tgcgttctgc acgatgcgag gaaggctttt
    35461 tgctaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt acactattgt
    35521 tccaggaatt atctatggtc cgtcgattac tcgatatccc tttcaattaa caatgagtca
    35581 tctccttccg gagcagacat tgggctgtat tgccacggat tatggtagct ctccatcgcc
    35641 tgatagatca cctgggccag ctcattatta ctggacgggt catagcacag caaatattcg
    35701 gtatcgggta gcggcggtaa gccatctact ccacttagga cacgcaggtc cggactcatc
    35761 atctcgaccg gccttgccgt cacgccaaga ccggctttca ctgccgcacg aacggccgga
    35821 agcgtcgagg cgacataagc cagtcgccag ggaatttctg ctttattaag tgtcgccagc
    35881 accatatcgc gaaacggact tggatcatcc agcaatacaa gagggatcgg ctcacctttt
    35941 tgcaatacgt attccgctgc gcagtaccag tgtgttggcg aggtacgcag gttcaatgcc
    36001 ttaaaggtag aaggacgatg ggtggttacc atcaaatcca cttcctggga ttccagcatt
    36061 tctgccatat aggcattacg tttaacgcgg acatccagcg ctaatttcgg ataaaccgaa
    36121 ctcacgcgat ttaacaggaa aggtaagatc gtatcggcag attcatctga agcaccgata
    36181 gttaacacgc cctgaagatt actgaacatt aatgatgagc aggcctcatc attaaaacgc
    36241 aggattttcc tggcgtaacc aagaagttga atgccatgtt cagttaacag tttgttgcga
    36301 ccgtgacgag cgaacagttc tttcccaacg agttgttcca gacgctgcat ttgctgactt
    36361 acggcggact gagtacgaca cacagcggca gctgcggcag caaaagtgtt cagatcggca
    36421 acagcaacaa atgttctcag cagatcgagg tcgaggttaa ttatcggacg atttgcactt
    36481 atcttatatt atcacttact ggcggctcat actgagctgg ttaatgctgt gcacacacaa
    36541 acaagcaatt ccatttgtaa tgtgcctccc tggcagtttc atccggaacc cggacgaaag
    36601 taaaaatgca tatgagttgt actaaaaaag cgactcacat tgttccgtta taatgcctga
    36661 agtagatcac agaatatatc ttcagggatc gcatatctat taagttactc actcttttct
    36721 atttatgaca tgcgcgtgtt tgtataaatg taaatgtgag tccttgttcc actctcgtgc
    36781 agcatcgctg gccatacgcg aacacgtaca aacagcaatg gtgaggcggc atcaagagca
    36841 gggatccgtc atttatgcga gcattttacc ccaaaaacct tttatttata agggtcattg
    36901 cgaattatct gatgcaaagt tatgttatgt taggcaaagt aatcttctat tattaataag
    36961 cacatcaaaa cctttttgaa tattaaataa taattaatta gcatcatcct cattcattaa
    37021 ttccgcttaa caatagtttc acaccttgcc cccattacga cacctcggaa atcatcacgc
    37081 tgttaaactc tccgtaatgc ggaaatatca tcatcgcgtt tcattagtga attcttaaat
    37141 gaggcatttt cacacaatta tcttacagat aaaaaaacca gacttacaat taagaatcag
    37201 aacaattcac tatataacat tgcatgtaaa gcatatacac ctcattattt tgtcattatt
    37261 aagttattaa cagcacaatc gagccttccc ctctggcaaa atcttattct gcagaccttc
    37321 aaaacaccgt cctgggggag tacattgttc taagctgact tccacggcag ggagtggcga
    37381 taacagcaaa aaaggtcaag attcatgtcc cccattgaaa aatccagcaa attagagaat
    37441 gtctgttatg acatccgtgg tccggtgctg aaagaagcaa aacgcctgga agaagaaggt
    37501 aacaaggtac tgaaactgaa catcggcaac ccagccccgt tcggttttga cgcgccagat
    37561 gaaatcctcg ttgacgtgat acgcaacctg cctaccgctc aagggtattg cgattccaaa
    37621 ggtctttact ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg catgcgtgat
    37681 gttaccgtgg aagatattta catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg
    37741 caggcattgc tgaacagcgg ggacgaaatg ttggttcctg caccagatta cccactatgg
    37801 accgcggcgg tttcgctttc cagcggtaaa gcggtgcatt atctttgcga tgaatcctct
    37861 gactggttcc cggacctcga tgatattcgc gctaaaatta cgcctcgtac gcgtgggatc
    37921 gttattatca acccaaataa cccaaccggc gcggtatatt ccaaagagct tttaatagag
    37981 attgtggaga ttgcacgtca gcataatctc attatcttcg ccgatgaaat ttatgacaaa
    38041 attctctacg acgacgctga gcatcactca attgcgccac tggcacctga cctgctgacc
    38101 attaccttta acggactgtc gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg
    38161 gtgttgaacg ggccgaaaaa acacgccaaa ggctacatcg aaggtctgga aatgctggct
    38221 tcaatgcgcc tgtgtgctaa cgttcctgca caacacgcca ttcagaccgc gctgggtggt
    38281 tatcagagca tcagtgaatt tattacccct ggcggtcgtc tttatgagca gcgtaaccgc
    38341 gcgtgggaac tgatcaacga tattccgggc gtttcctgcg tgaaacctcg tggtgcgctg
    38401 tatatgttcc cgaaaatcga cgccaaacgc tttaacattc acgacgatca gaaaatggtg
    38461 ctggatttcc tgttgcagga aaaagttctg ttggtgcaag ggacggcatt caactggccg
    38521 tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg
    38581 agcaagttcg cgcgtttcct ttctggttat catcagctgt aatcttaatt tcactgccgg
    38641 agattgcatc cggcagcgtt atcccgccac aatgacctga tgatgtcatc atacgtaagg
    38701 tcactatgaa acagagccat ttttttgccc atctctcccg cctgaaactc attaaccgct
    38761 ggccgctcat gcgcaacgtg cggacggaaa atgtgtccga acacagtttg caggtagcga
    38821 tggtcgccca tgcgctggca gctatcaaaa atcgaaaatt tggcggtaat gtcaacgccg
    38881 aacgtatcgc tttactggcg atgtaccacg atgcctcaga agtgctcacc ggcgatctcc
    38941 ctactccggt gaaatacttc aattcgcaaa tcgctcagga gtacaaggct attgaaaaaa
    39001 tcgctcagca aaaactggtc gatatggttc cggaagagct gcgggatatc tttgcgccgt
    39061 taattgacga gcatgcatat agcgatgaag aaaaatcgct ggtgaaacag gcagatgcac
    39121 tgtgtgcata tctgaaatgt ctggaagaac tcgcggccgg aaataatgaa ttcttgctgg
    39181 caaaaacgcg actggaagcg acgcttgaag cgcgtcgcag ccaggagatg gactacttca
    39241 tggaaatatt tgttcccagc ttccatcttt cgctcgatga gattagccag gattcaccgc
    39301 tgtaagcagc cggagtctgc gtcgcatcag gcaataagcg ccggatgcga catcaggctc
    39361 ttgtcaaaac ggaaacagca tcgggatcat caccacacaa accgccatca cgataatggt
    39421 gaacggtacc cccaacttca caaagtcact gaagctgtaa tttcccggac ctaaaaccag
    39481 tgtgttaaca ggtgaagaaa ccggtgtcat aaaggcggcg gatgctgcca tcgccacgac
    39541 catcgcgaat ggataaggcg acacccccat cgttttggca gcagccagcg caatcggagc
    39601 catcaacacc gccgtcgcgg tattagagat aaatagccca ataaccgccg acaagacaaa
    39661 caaacacccc agcatcatat gtggcccg
//
LOCUS       DAOFQD010000029        38822 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.029, whole genome shotgun sequence.
ACCESSION   DAOFQD010000029 DAOFQD010000000
VERSION     DAOFQD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.029"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(146..1621)
                     /gene="ampG"
                     /locus_tag="QBF46_001609"
     CDS             complement(146..1621)
                     /gene="ampG"
                     /locus_tag="QBF46_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDQ6673802.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1665..2243)
                     /locus_tag="QBF46_001610"
     CDS             complement(1665..2243)
                     /locus_tag="QBF46_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6673803.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2548..2865
                     /gene="bolA"
                     /locus_tag="QBF46_001611"
     CDS             2548..2865
                     /gene="bolA"
                     /locus_tag="QBF46_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDQ6673804.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3209..4507
                     /gene="tig"
                     /locus_tag="QBF46_001612"
     CDS             3209..4507
                     /gene="tig"
                     /locus_tag="QBF46_001612"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDQ6673805.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4753..5376
                     /gene="clpP"
                     /locus_tag="QBF46_001613"
     CDS             4753..5376
                     /gene="clpP"
                     /locus_tag="QBF46_001613"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDQ6673806.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5502..6776
                     /gene="clpX"
                     /locus_tag="QBF46_001614"
     CDS             5502..6776
                     /gene="clpX"
                     /locus_tag="QBF46_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDQ6673807.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6964..9318
                     /gene="lon"
                     /locus_tag="QBF46_001615"
     CDS             6964..9318
                     /gene="lon"
                     /locus_tag="QBF46_001615"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDQ6673808.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9527..9799
                     /gene="hupB"
                     /locus_tag="QBF46_001616"
     CDS             9527..9799
                     /gene="hupB"
                     /locus_tag="QBF46_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDQ6673809.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            9991..11862
                     /gene="ppiD"
                     /locus_tag="QBF46_001617"
     CDS             9991..11862
                     /gene="ppiD"
                     /locus_tag="QBF46_001617"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDQ6673810.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12013..12384
                     /locus_tag="QBF46_001618"
     CDS             12013..12384
                     /locus_tag="QBF46_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDQ6673811.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12490..12888
                     /gene="fadM"
                     /locus_tag="QBF46_001619"
     CDS             12490..12888
                     /gene="fadM"
                     /locus_tag="QBF46_001619"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDQ6673812.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSESFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12940..13635)
                     /gene="queC"
                     /locus_tag="QBF46_001620"
     CDS             complement(12940..13635)
                     /gene="queC"
                     /locus_tag="QBF46_001620"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDQ6673813.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGMKGDGCGHCAACNLRTNGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13700..15400)
                     /locus_tag="QBF46_001621"
     CDS             complement(13700..15400)
                     /locus_tag="QBF46_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDQ6673814.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTISELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELIIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15500..16318
                     /gene="cof"
                     /locus_tag="QBF46_001622"
     CDS             15500..16318
                     /gene="cof"
                     /locus_tag="QBF46_001622"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDQ6673815.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16471..16929
                     /gene="decR"
                     /locus_tag="QBF46_001623"
     CDS             16471..16929
                     /gene="decR"
                     /locus_tag="QBF46_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDQ6673816.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16959..18731
                     /locus_tag="QBF46_001624"
     CDS             16959..18731
                     /locus_tag="QBF46_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDQ6673817.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            18724..20505
                     /locus_tag="QBF46_001625"
     CDS             18724..20505
                     /locus_tag="QBF46_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDQ6673818.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDNNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20686..21024
                     /gene="glnK"
                     /locus_tag="QBF46_001626"
     CDS             20686..21024
                     /gene="glnK"
                     /locus_tag="QBF46_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDQ6673819.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21054..22340
                     /gene="amtB"
                     /locus_tag="QBF46_001627"
     CDS             21054..22340
                     /gene="amtB"
                     /locus_tag="QBF46_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDQ6673820.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22389..23249)
                     /gene="tesB"
                     /locus_tag="QBF46_001628"
     CDS             complement(22389..23249)
                     /gene="tesB"
                     /locus_tag="QBF46_001628"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDQ6673821.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23467..24039
                     /locus_tag="QBF46_001629"
     CDS             23467..24039
                     /locus_tag="QBF46_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDQ6673822.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24070..24381)
                     /locus_tag="QBF46_001630"
     CDS             complement(24070..24381)
                     /locus_tag="QBF46_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDQ6673823.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24544..24640
                     /gene="ffs"
                     /locus_tag="QBF46_001631"
     ncRNA           24544..24640
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="QBF46_001631"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24760..25113
                     /locus_tag="QBF46_001632"
     CDS             24760..25113
                     /locus_tag="QBF46_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDQ6673824.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25155..26705)
                     /gene="pdeB"
                     /locus_tag="QBF46_001633"
     CDS             complement(25155..26705)
                     /gene="pdeB"
                     /locus_tag="QBF46_001633"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDQ6673825.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVAYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLRDMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26869..27339)
                     /locus_tag="QBF46_001634"
     CDS             complement(26869..27339)
                     /locus_tag="QBF46_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDQ6673826.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27455..28006)
                     /gene="maa"
                     /locus_tag="QBF46_001635"
     CDS             complement(27455..28006)
                     /gene="maa"
                     /locus_tag="QBF46_001635"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDQ6673827.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28178..28396)
                     /gene="hha"
                     /locus_tag="QBF46_001636"
     CDS             complement(28178..28396)
                     /gene="hha"
                     /locus_tag="QBF46_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDQ6673828.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28422..28796)
                     /gene="tomB"
                     /locus_tag="QBF46_001637"
     CDS             complement(28422..28796)
                     /gene="tomB"
                     /locus_tag="QBF46_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDQ6673829.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29342..32491)
                     /locus_tag="QBF46_001638"
     CDS             complement(29342..32491)
                     /locus_tag="QBF46_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDQ6673830.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(32514..33707)
                     /gene="acrA"
                     /locus_tag="QBF46_001639"
     CDS             complement(32514..33707)
                     /gene="acrA"
                     /locus_tag="QBF46_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDQ6673831.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33849..34496
                     /gene="acrR"
                     /locus_tag="QBF46_001640"
     CDS             33849..34496
                     /gene="acrR"
                     /locus_tag="QBF46_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDQ6673832.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34624..37986
                     /gene="mscK"
                     /locus_tag="QBF46_001641"
     CDS             34624..37986
                     /gene="mscK"
                     /locus_tag="QBF46_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDQ6673833.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWQKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLAGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(38198..38359)
                     /gene="rsmS"
                     /locus_tag="QBF46_001642"
     CDS             complement(38198..38359)
                     /gene="rsmS"
                     /locus_tag="QBF46_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDQ6673834.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(38373..>38822)
                     /gene="priC"
                     /locus_tag="QBF46_001643"
     CDS             complement(38373..>38822)
                     /gene="priC"
                     /locus_tag="QBF46_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDQ6673835.1"
                     /translation="FATLSARFDRHLFQTRATTLQACLDEAGDNLAALRHAVEQQQLP
                     QVAWLAEHLAAQLEAIAREASAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAE
                     RRARLVRVTDLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR"
BASE COUNT         9656 a         9565 c        10329 g         9272 t
ORIGIN      
        1 aagaaaaaat agaattcata aattgcgtca ataatttcca aacgctaagc cgcacaaaag
       61 aacaaatatt attaagcgat actgatgttt atttagccgt aataattacg gcgagtgatt
      121 actacagcta aataatattt acagattacg tcagatgcgt ttttcgtagc gccagataat
      181 ccagcaaacc accaagcacg acgccagaaa gggcgactaa aaccccgact tccagcagag
      241 caggcaggaa agagaagtgc gtcaaatcca gcgcgtccat cgtcaacagc agtaaccaca
      301 cggccaacag gctgacgccc gccgccagtg tccacatggc aaaggcataa cctgccggat
      361 attcggtacg ggagataaag ttgtcattta ctcgtgtata ttcaagcgtc tggcggcaaa
      421 ccagcagcaa aataagcccc ggtacggcag cggcgacgga gaatagatag aatgtcgacc
      481 agccgtgtgc ttcaacaaac caacccgcca cggggccgac ataaactcgt cctacagcgg
      541 aaagcgctga gagcagggca aattgagtag cggaaaatga cttattacac agcgtcatta
      601 acagcgcgac aaaggctgat gtgcccatcc cgccacagag gttttcgaaa aagacggctg
      661 cgcccatgct gtagagatgc ttatcagtaa tcgacagcag ccagtaacca gcgttagacg
      721 caccttgtaa aatgccgaaa atcagcagcg cccggaacag cgacaggcgc tgcatcaaaa
      781 tcccaccgta caatgcgccc acaatggtcg ccagtaagcc aagcgttttg tttaccacgc
      841 ctacttcacc cgcatcaaac ccgacgccgc gaatcaaaaa cgtggttgtc aggctcatgg
      901 cgaatgcgtc gcccagctta tacagcacga taagaagcaa aataagccag gcattattgc
      961 gaccaaagaa atctcgcaga ggtgcaacaa ccgcttgttc cagcgttttg ggcacaggaa
     1021 tggtgtcggt tggttctggt gcaagcaacg tcgcgataat acaggggatc aacagcgccg
     1081 ccatcaacca gtacatgccc tgccagccca gccatttatc tgccagccac agggctaggc
     1141 cgccggaaac cagcatccct aaacggtaac ccagcacgct gattgccgcg cccgcaccac
     1201 gctcttctgc cgggagcaca tcggttttcc acgcatcgaa gacaatatcc tgagaggccg
     1261 agcaaaaagc gatcaccacc gccagcgccg ccatccagcg gagttgggtg cctggttcga
     1321 gaaaacccat cgccgcaatg gcgactaata acaggatttg cgtggcgagc agccaaccgc
     1381 gccgccgccc aaaaaatggg ggcgtgtagc ggtccatcag cggtgaccag aggaatttaa
     1441 aaacgtaagc ctggcctacc agagagaaga aaccaatggt tttgagatca atattctcga
     1501 ccgtcatcca ggcctgtaag gtaccggatg tcagggcgag cggtagcccg gaagcaaaac
     1561 ccaggatcag caatatggct gaacgcggct gttgaaaaat acgtaaatat tgactggaca
     1621 tgggcgtcta caggcccggc ttgcaccggg ccagagggca gaaattaacg cgcgttctgc
     1681 ttgatgaatt cgtggatgct ggtgtcctga gacatatcag cgatggtatc ggtcagcaca
     1741 ctgttaaccg catcggcgat atttttgttg gaggcctgga acgcaccttc aacgttgtag
     1801 ctggcacgat agtttttggt cattttgttg ccattctgcg cggtagcgat gatggcgata
     1861 tccgctttgg tcgcgatgtt gtagcgcacg ttgccctggg acacgtcagc atacagttgg
     1921 ctaacgatga tttgcagatt aaccgggcca ttcggaccaa ccatgtaacc acgcgcggtc
     1981 atctgttttt ccagcacttc ttgcagcagg aaacgcagat cgcgggaggc ggtcagggta
     2041 acgatttgat tatcgcgggt gacttttgcc agcgcctgat cggtacgctg atcggcacca
     2101 ttaatgctta cggtgacgcc catcaggctt ggatcctgct gtggcagtgt aatcgtcggg
     2161 gaaacttcaa tagttgttgg cggttttgcg catcctgcaa gcataaacag agcaactaac
     2221 gggaagagga tttttttgaa catgttcggg ctctcagaga ctcttaagcg tgtttggtaa
     2281 aaattcccgc catcataaca ttgccaacgg cgaggggaag tgggtaaggc atgtaaattc
     2341 atcatgttga cgaaataatc gcccctggta aaagaaacac tgatgcgagg cctgtgtttc
     2401 aatctttaaa tcagtaaact tcatacgctt gacggaaaaa ccaggacgaa acctaaatat
     2461 ttgttgttaa gctgcaatgg aaacggtaaa agcggctagt atttaaaggg atggatgaca
     2521 tctcagcgtt gtcggaggag atatttcatg atgatacgtg agcggataga agaaaaatta
     2581 agggcggcgt tccaacccgt attcctcgaa gtagtggatg aaagctatcg tcacaatgtc
     2641 ccagccggct ctgaaagcca ttttaaagtt gtgctggtca gcgatcgttt tacgggtgaa
     2701 cgttttctga atcgtcatcg aatgatttac agtactttag cggaggaact ctctactacc
     2761 gttcatgcgc tggctctgca tacttacact attaaggagt gggaagggtt gcaggacacc
     2821 gtctttgcct ctcctccctg tcgtggagca ggaagcatcg cgtaaaaacg catttgcaac
     2881 tgtcggcgct tttccagtat gttgctaaag attttatgaa aaacggcctg cgggccgttt
     2941 tgttttgtct ggattttgcg ctttttgccc agcattcaga cgaaaattgc ccgggaattg
     3001 tgaaaaaata cgcgacagcg cgcaataacc gttctcgact cataaaagtg atgccgctat
     3061 aatgccgcgt cctatttgaa tgctttcggg atgattctgg taacagggaa tgtgattgat
     3121 tataagaaca tcccggttcc gcgaagccaa caacctgtgc ttgcggggta agagttgacc
     3181 gagcactgtg attttttgag gtaacaagat gcaagtttca gttgaaacca ctcaaggcct
     3241 tggccgccgt gtaacgatta ctatcgctgc tgacagcatc gagaccgctg ttaaaagcga
     3301 gctggtcaac gttgcgaaaa aagtacgtat tgacggcttc cgcaaaggca aagtgccaat
     3361 gaatatcgtt gctcagcgtt atggcgcgtc tgtacgccag gacgttctgg gtgacctgat
     3421 gagccgtaac ttcattgacg ccatcattaa agaaaaaatc aatccggctg gcgcaccgac
     3481 ttatgttccg ggcgaataca agctgggtga agacttcact tactctgtag agtttgaagt
     3541 ttatccggaa gttgaactgc agggtctgga agcgatcgaa gttgaaaaac cgatcgttga
     3601 agtgaccgac gctgacgttg acggcatgct ggatactctg cgtaaacagc aggcgacctg
     3661 gaaagaaaaa gacggcgctg ttgaagcaga agaccgcgta accatcgact tcaccggttc
     3721 tgtagacggc gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg cgatgggcca
     3781 gggtcgtatg atcccgggct ttgaagacgg tatcaaaggc cacaaagctg gcgaagagtt
     3841 caccatcgac gtgaccttcc cggaagaata ccacgcagaa aacctgaaag gtaaagcagc
     3901 gaaattcgct atcaacctga agaaagttga agagcgtgaa ctgccggaac tgactgcaga
     3961 attcatcaaa cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg ctgaagtgcg
     4021 taaaaacatg gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt ctcaggcgat
     4081 cgaaggtctg gtaaaagcta acgacatcga cgtaccggct gcgctgatcg acagcgaaat
     4141 cgacgttctg cgtcgccagg ctgcacagcg tttcggtggc aacgaaaaac aagctctgga
     4201 actgccgcgc gaactgttcg aagaacaggc taaacgccgc gtagttgttg gcctgctgct
     4261 gggcgaagtt atccgcacca acgagctgaa agctgacgaa gagcgcgtga aaggcctgat
     4321 cgaagagatg gcttctgcgt acgaagatcc gaaagaagtt atcgagttct acagcaaaaa
     4381 caaagaactg atggacaaca tgcgcaatgt tgctctggaa gaacaggctg ttgaagctgt
     4441 actggcgaaa gcgaaagtga ctgaaaaaga aaccactttc aacgagctga tgaaccagca
     4501 ggcgtaattt acgcagcata acgcgctaaa ttcgcacaaa ggcccgtcac cgccaggtgg
     4561 tgggcttttt tttgtcatga attttgcatg gaaccgtgcg aaaagcctct ttcggtgtta
     4621 gcgtaacaac aaaagattgt tatgcttgaa atatggtgat gccgtaccca taacacaggg
     4681 actagctgat aatccgtcca taaggttaca atcggtacag caggtttttt caattttatc
     4741 caggagacgg aaatgtcata cagcggcgaa cgagataact ttgcacccca tatggcgctg
     4801 gtgccgatgg tcattgaaca gacctcacgc ggtgagcgct cttttgatat ctattctcgt
     4861 ctacttaagg aacgcgtcat ttttctgact ggccaggttg aagaccacat ggctaacctg
     4921 attgtggcgc agatgctgtt cctggaagcg gaaaacccag aaaaagatat ctatctgtac
     4981 attaactccc caggcggggt gatcactgcc gggatgtcta tctatgacac catgcagttt
     5041 atcaagcctg atgtcagcac tatctgtatg ggccaggcgg cctcgatggg cgctttcttg
     5101 ctgaccgcag gggcaaaagg taaacgtttt tgcctgccga attcgcgcgt gatgattcac
     5161 caaccgttgg gcggctacca gggccaggcg accgatatcg aaattcatgc ccgtgaaatt
     5221 ctgaaagtta aagggcgcat gaatgaactt atggcgcttc atacgggtca atcattagaa
     5281 cagattgaac gtgataccga gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac
     5341 ggtctggtcg attcgattct gacccatcgt aattgatgcc agaggcgcaa ctgtgccgct
     5401 atacttatcc agggcggcac aacgctgtaa gcggcttgcg cctgagaatg gcatttgcgt
     5461 cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc catgacagat aaacgcaaag
     5521 atggctcagg caaattgctg tattgctctt tttgcggcaa aagccagcat gaagtgcgca
     5581 agctgattgc cggtccatcc gtgtatatct gcgacgaatg tgttgattta tgtaacgaca
     5641 tcattcgcga agagattaaa gaagttgcac cgcatcgtga acgcagtgcg ctaccgacgc
     5701 cgcatgaaat tcgcaaccac ctggacgatt acgttatcgg ccaggaacag gcgaaaaaag
     5761 tgctggcggt cgcggtatac aaccattaca aacgtctgcg caacggcgat accagcaatg
     5821 gcgtcgagtt gggcaaaagt aacattctgc tgatcggtcc gaccggttcc ggtaaaacgc
     5881 tgctggctga aacgctggcg cgcctgctgg atgttccgtt caccatggcc gacgcgacta
     5941 cactgaccga agccggttat gtgggtgaag acgttgaaaa catcattcag aagctgttgc
     6001 agaaatgcga ctacgatgtc cagaaagcac agcgtggtat tgtctacatc gatgaaatcg
     6061 acaagatttc tcgtaagtca gacaacccgt ccattacccg agacgtttcc ggtgaaggcg
     6121 tacagcaggc actgttgaaa ctgatcgaag gtacggtagc tgctgttcca ccgcaaggtg
     6181 ggcgtaaaca tccgcagcag gaattcttgc aggttgatac ctctaagatc ctgtttattt
     6241 gtggcggtgc gtttgccggt ctggataaag tgatttccca tcgtgtagaa accggctccg
     6301 gcattggttt tggcgcgacg gtaaaagcga agtccgacaa agcaagcgaa ggcgagctgc
     6361 tggcgcaggt tgaaccggaa gatctgatca agtttggtct gatccctgag ttcattggtc
     6421 gtctgccggt tgtcgcaacg ttgaatgaac tgagcgaaga agctctgatt cagatcctca
     6481 aagagccgaa aaacgccctg accaagcagt atcaggcgct gtttaatctg gaaggcgtgg
     6541 atctggaatt ccgtgacgag gcgctggatg ctatcgctaa gaaagcgatg gcgcgtaaaa
     6601 ccggtgcccg tggcctgcgt tccatcgtag aagccgcact gctcgatacc atgtacgatc
     6661 tgccgtccat ggaagacgtc gaaaaagtgg ttatcgacga gtcggtaatt gatggtcaaa
     6721 gcaaaccgtt gctgatttat ggcaagccgg aagcgcaaca ggcatctggt gaataattaa
     6781 ccattcccat acaattagtt aaccaaaaag gggggatttt atctcccctt taatttttcc
     6841 tctattctcg gcgttgaatg tgggggaaac atccccatat actgacgtac atgttaatag
     6901 atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg aaattaaact aagagagagc
     6961 tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg tattgccgct gcgcgatgtg
     7021 gtggtttatc cgcacatggt catcccctta tttgtcgggc gggaaaaatc tatccgttgt
     7081 ctggaagcgg cgatggacca tgataaaaaa attatgctgg tcgcgcagaa agaagcttca
     7141 acggatgagc cgggtgtaaa cgatcttttc accgtcggga ccgtggcctc tatattgcag
     7201 atgctgaagc tgcctgacgg caccgtcaaa gtgctggtcg aggggttaca gcgcgcgcgt
     7261 atttctgcgc tctctgacaa tggcgaacac ttttctgcga aggcggagta tctggagtcg
     7321 ccgaccattg atgagcgaga acaggaagtg ctggtgcgta ctgcaatcag ccagttcgaa
     7381 ggctacatca agctgaacaa aaaaatccca ccagaagtgc tgacgtcgtt gaatagcatc
     7441 gacgatccgg cgcgtctggc ggataccatt gctgcacata tgccgctgaa actggctgac
     7501 aaacagtccg ttctggagat gtccgacgtt aacgaacgtc tggaatatct gatggcaatg
     7561 atggaatcgg aaatcgatct gctgcaggtt gagaaacgca ttcgcaaccg cgttaaaaag
     7621 cagatggaga aatcccagcg tgagtactat ctgaacgagc aaatgaaagc tattcagaaa
     7681 gaactcggtg aaatggacga cgcgccggac gaaaacgaag ccctgaagcg caaaatcgac
     7741 gcggcgaaga tgccgaaaga agcaaaagag aaagcggaag cagagttgca gaagctgaaa
     7801 atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg gttatatcga ctggatggta
     7861 caggtaccgt ggaatgcgcg cagcaaggtc aaaaaagacc tgcgtcaggc gcaggaaatc
     7921 cttgataccg accattatgg tctggagcgc gtgaaagatc gcatccttga gtaccttgcg
     7981 gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct gcctggtagg gccgccgggg
     8041 gtaggtaaaa cctctcttgg tcagtccatc gccaaagcca ccgggcgtaa atatgtccgt
     8101 atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg gtcaccgccg tacttacatc
     8161 ggttctatgc cgggtaaact gatccagaaa atggcgaaag tgggcgtgaa aaacccgctg
     8221 ttcctgctcg atgagatcga caaaatgtct tctgacatgc gaggcgatcc ggcctctgca
     8281 ctgcttgaag tgctggatcc agagcagaac gtagcgttca gcgaccacta cctggaagtg
     8341 gattacgatc tcagcgacgt gatgtttgtc gcgacgtcga actccatgaa cattccggca
     8401 ccgctgctgg atcgtatgga agtgattcgc ctctccggtt ataccgaaga tgaaaaactg
     8461 aacatcgcca aacgtcacct gctgccgaag cagattgaac gtaatgcact gaaaaaaggt
     8521 gagctgaccg tcgacgatag cgccattatc ggcattattc gttactacac ccgtgaggcg
     8581 ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgcc gcaaagcggt taagcagtta
     8641 ctgctcgata agtcattaaa acatatcgaa attaacggcg ataacctgca tgactacctc
     8701 ggtgttcagc gtttcgacta tggtcgcgct gataacgaaa accgtgtcgg tcaggtaacc
     8761 ggtctggcgt ggacggaagt gggcggtgac ttgctgacca ttgaaaccgc atgtgttccg
     8821 ggtaaaggca aactgaccta taccggttcg ctcggcgaag tgatgcagga gtctattcag
     8881 gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg ggatcaaccc tgatttttac
     8941 gaaaaacgtg acatccacgt ccacgtaccg gaaggtgcga cgccgaaaga tggtccgagt
     9001 gccggtattg ctatgtgcac cgcgctggtt tcttgcctga ccggtaaccc ggttcgtgcc
     9061 gatgtggcaa tgaccggtga gatcactctg cgtggtcagg tactgccgat cggtggtttg
     9121 aaagaaaaac tcctggcagc gcatcgcggc gggattaaaa cagtgctaat tccgttcgaa
     9181 aataaacgcg atctggaaga gattcctgac aacgtaattg ccgatctgga cattcatcct
     9241 gtgaagcgca ttgaggaagt tctgactctg gcgctgcaaa atgaaccgtc tggcatgcag
     9301 gttgtgactg caaaatagtg acctcgcgca aaatgcacta ataaaaacag ggctggcagg
     9361 ctaattcggg cttgccagcc tttttttgtc tcgctaagtt agatggcgga tcgggcttgc
     9421 ccttattaag gggtgttgta aggggatggc tggcctgata taactgctgc gcgttcgtac
     9481 cttgaaggat tcaagtgcga tataaattat aaagaggaag agaagagtga ataaatctca
     9541 attgatcgac aagattgctg caggggctga tatctctaaa gctgcggctg gccgtgcgtt
     9601 agatgctatt attgcttccg taactgaatc tctgaaagaa ggggatgatg tagcactggt
     9661 aggttttggt acttttgccg ttaaagagcg tgctgcccgt actggccgca acccgcagac
     9721 cggtaaagag atcaccatcg ctgccgctaa agtaccgagc ttccgtgcag gtaaagcact
     9781 gaaagacgcg gtaaactaag cgttgtcccc agtggggatg tgacgaagtt caagggcgca
     9841 tctactgatg tgcctttttt atttgtattc ggtgactttc tgtgtcttgt gggctgacaa
     9901 ttgcccccgt ttcttgtcac aataggcctt tgcgcgcatc gatacgttgc gtgaggtaca
     9961 cagtcatcta cagcggagtg ttgttacacc atgatggaca gcttacgcac ggctgcaaac
    10021 agtctcgtgc tcaagattat tttcggtatc attatcgtgt cgttcatatt gaccggcgtg
    10081 agtggttacc tgattggcgg aggcaataac tacgccgcaa aagtgaatga ccaggaaatc
    10141 agccgtgggc aattcgagaa cgccttcaac agcgagcgta atcgcatgca gcaacagctg
    10201 ggcgatcaat actccgagct ggcagcgaac gaaggctata tgaaaaccct gcgtcaacag
    10261 gtgctgaatc gtctgatcga cgaggcgctg ctggatcagt acgcacgtga gctgaaactg
    10321 ggtatcagcg atgagcaggt taaacaggcg attttcgcga ccccagcctt ccaggttgac
    10381 ggcaaatttg ataacagccg ctataacggt atcctcaacc agatggggat gaccgccgat
    10441 cagtacgccc aggcgctgcg taaccagctc actacccaac agctgattaa cggcgttgcc
    10501 ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg cggcactggt cgcgcaacaa
    10561 cgcgtggtgc gtgaggcgac tatcgatgtt aacgcgctgg cggcgaagca gcctgtgacc
    10621 gaacaggaga ttgccagcta ctacgaacaa aacaaaaaca atttcatgac gccggaacaa
    10681 ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc agcaaccggt tagcgatgcg
    10741 gatatccaga gctactacga ccagcatcag gatcaattca cccagccgca gcgtacccgc
    10801 tacagcatca tccagaccaa aactgaagat gaagcgaaag cggtacttga tgagctgaat
    10861 aaaggcggtg attttgctgc attagccaaa gaaaaatctg ccgatattat ctctgctcgt
    10921 aacggcggcg atatgggttg gttagaagat gccactatcc cggacgaact gaaaaatgct
    10981 ggtctgaaag aaaaaggcca actgtctggt gtcatcaaat cttcggtcgg tttcctgatt
    11041 gtacgtctgg acgacattca gccagcgaaa gtgaaatcgt tagacgaagt acgtgacgac
    11101 attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt actacgcgct gcagcagaaa
    11161 gtgagcgatg cggcaagcaa cgacaccgag tctctggccg gtgcagagca agctgccggc
    11221 gttaaagcca ctcagacggg ttggttcagc aaagataacc tgccggaaga gttgaacttc
    11281 aagccggttg ccgacgctat ctttaacggc ggtctggtag gtgaaaacgg cgcgccgggc
    11341 atcaactctg acatcatcac cgtagacggc gaccgcgcat tcgtgctgcg catcagcgag
    11401 cacaaaccgg aagcggtgaa accgttggca gatgttcagg aacaagttaa ggcactggtt
    11461 cagcacaaca aagctgaaca acaggcgaaa gtggatgctg agaaactgct ggttgatttg
    11521 aaagccggca aaggtgcgga agctatgcag gctgccggtc tgaaatttgg agagccgaaa
    11581 accttaagcc gttccggtcg tgacccgatt agccaggcgg cgtttgcact gccactgcca
    11641 gcgaaagaca aaccgagcta cggtatggcg accgatatgc aaggcaatgt ggttctgctg
    11701 gcgctggatg aagtgaaaca aggttcaatg ccggaagatc agaaaaaagc gatggtgcag
    11761 ggtatcaccc agaacaacgc acaaatcgtc tttgaagctc tgatgagtaa cctgcgtaaa
    11821 gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat aatcctgaag ccgcctcgca
    11881 aaaaaatgct ttgcagctgt agcaagaaaa ggtcgctttc gcggcctttt ccatttctga
    11941 acattgccat ttgtttactg ttttcactgc cgttaaggtg attccactgt taacaaacaa
    12001 ggagaaaaca gtatgaaaca cggaattaaa gcactgctca ttaccctgtc tctggcttgt
    12061 gccggaatgt ctcatagcgc gctggcggca gctcctgcgg cgaaaccgac aacggtagaa
    12121 accaaagcgg aagctcctgc agcacaaagt aaagcagcag taccggcgaa agccagtgac
    12181 gaagaaggca cccgggtcag cattaataat gccagcgcgg aagagctagc ccgcgcgatg
    12241 aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt atcgcgaaga gtacggtccg
    12301 tttaaaacgg tggaggatct aaagcaggta ccggggatgg gcaattcgct ggtggagcgt
    12361 aatctggcgg tattaaccct gtaattaatt tgcatagtgg caattttttg ccagactgaa
    12421 gaggtcatac cagttatgac ctctgtactt ataacagttc ttataacaac aacgtaaggt
    12481 tattgcgcta tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac
    12541 gtcaacaacg ctcgctacct tgaattcctc gaagaagccc gctgggatgg gttggaaaat
    12601 agtgagagtt ttcagtggat gacggcccat aacatcgcct tcgtggtggt gaatatcaat
    12661 attaactatc gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag
    12721 caattaaacg gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag
    12781 gtggtagcag atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta
    12841 gctctggaag gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt
    12901 gccggatgct caagccgcat ccggcgacac ccggaataat tacttcaacc cggttttctg
    12961 cttcatcgcg gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggtgcg
    13021 taaattacat gccgcacaat gaccgcaacc gtcgcctttc atgccgttat agcaggtcaa
    13081 cgtttcgtta cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc
    13141 tttatcaatc cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac
    13201 ggcatggttt agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt
    13261 ttcacagacg ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag
    13321 cgtcaggaac aaaatattac gccctgggac aaacgtattc gggatgccat cggcttcagg
    13381 ttcataatca ggcaccggaa tgctgtcacg cgtcaggcta ctgaccgcca gctcgttgag
    13441 cagggtgaca tccagcacct tatgcgcgcg tgcccccagt ttcagcgcca gttcgcgtgc
    13501 cacgtcgatt tctgcgcgat gccgctgacc gtaatcgaac gtcacgcaat ggacttcatc
    13561 atattgttgt aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac
    13621 gacagcacgt ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg
    13681 attaacccgg accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa
    13741 accagccgcg cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt
    13801 gatagttgaa cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca
    13861 gggcattaaa tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca
    13921 gatgtgcata tgctggagcg tcgaaaacat gtggccacag tggatcgcaa cgtagccatt
    13981 gctccagagt atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt
    14041 gtgccagtag ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt atgagttcac
    14101 agccttccgc tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta
    14161 ggtgataaac cagcgtcaag gttttcggta gtttgacttc atccggtact tgccaatgcg
    14221 gaatagtcca gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta
    14281 acgtttgtaa taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg
    14341 acagtcgggg acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga
    14401 cctggctgac ccgttgcagc tcctcaggtt tgccgatagt gatttgcacg ggatgccgac
    14461 aactggttcc caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa
    14521 gcagcggatg acgcaaatgg taataatcat ggctttccag gcgcaccagc tccgctgtga
    14581 attgtgttaa gcgaaaagga cccgtgccga tcagtgggaa ttgcggatgc gccagatggc
    14641 tgcaatagct cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca
    14701 gacactgcgg atgggtgacc tcaatacgct tcacgctaat aaataattga tccagtgctg
    14761 gcagttgtaa cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt
    14821 tatgccagtg tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt
    14881 cccagtgatg cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc
    14941 cggaaaatat ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta
    15001 gcggttcgag cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc
    15061 cgcccataaa cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca
    15121 gcacatcttg ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt
    15181 ccggcgtgac cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact
    15241 ccagccatcc cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa
    15301 aacagcgttc ggccagttcg ctgatggtga cggtttgcgg ctttccggcg gaaggttgcc
    15361 acagacgttg atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta
    15421 tttaactatt cactattact tccgtatata tcaggtgata ctcaatcacc attaaccgtg
    15481 tcacagagtg gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt
    15541 gatgcctgac catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg
    15601 cgacattacc ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg
    15661 ggcgctatcg ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga
    15721 aggtgaactt ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca
    15781 gcaatgggat acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa
    15841 agagatccct gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga
    15901 tgtcaaaaaa atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga
    15961 tcttacacgc ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt
    16021 ttccgccacg gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac
    16081 ggtgctgacc caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat
    16141 gaacgatcgc gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc
    16201 gcaactgcgc gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt
    16261 ctctcactat ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataatg
    16321 agatcccttc cagcaccggg caattgcccg gttttttttg cgttgaattt gtcattttgt
    16381 gccgtggtgt ttaaaccgca cagaataaat tgtcgtgatt tcacctttaa aatagaatta
    16441 aaagagaaaa aaattctctg tggaagggct atgttagata aaattgaccg taagctgctg
    16501 gccttactgc agcaggattg caccctctct ttgcaggcac tggctgaagc cgttaatctg
    16561 acaaccaccc cttgctggaa gcgcctgaaa cggctggagg acgacggtat ccttatcggc
    16621 aaagtcgccc tgctggatcc ggaaaaaata ggcctcggcc tgaccgcttt tgtgctgata
    16681 aaaacgcaac atcacagcag cgaatggtat tgccgctttg tcacggtggt taccgaaatg
    16741 ccagaagtgc tggggttctg gcgcatggcc ggtgaatacg attatctgat gcgcgtccag
    16801 gttgccgaca tgaaacgcta cgacgagttt tataagcgtc tggtaaacag cgtgccgggg
    16861 ctgtcggacg tcacttccag cttcgcgatg gaacagatta aatacaccac ttctttaccc
    16921 atcgaataaa tatccagaat caggtcagga cacaacgcgt gcgattattt gctcaattaa
    16981 gctggtattt ccgtcgggaa tggcgtcgct atctcggggc tgtcgccttg cttgtcatta
    17041 tcgcgatgct gcaactggtt ccaccaaaag tggttggtat tgttgtcgat ggcgtgacag
    17101 aacaacactt tactaccggg cagatcctga tgtggatcgc caccatggtg ctgattgccg
    17161 ttgtggttta tctcctgcgt tacgtctggc gcgtattgct gtttggtgcg tcttatcaac
    17221 tggctgttga actgcgtgaa gattattacc gtcagctaag ccggcagcat cctgagtttt
    17281 acctgcgtca tcgcacgggt gacctcatgg ctcgtgcgac taatgacgtc gatcgcgtcg
    17341 tgtttgccgc cggagaaggg gtgctgacgc tggtggattc actggtaatg ggctgcgctg
    17401 tgttgattat gatgtctacg caaattagct ggcagttgac cttatttgcc ctgttaccga
    17461 tgccagtgat ggcgatcatg attaagcgca acggcgatgc tttgcatgaa cgctttaagc
    17521 tggcacaggc ggcgttttcc agtcttaatg accgcaccca ggaaagcctc accagtatcc
    17581 gcatgatcaa agcctttggt ctggaagatc gccagtcggc gttatttgcc gcggatgccg
    17641 aagataccgg caaaaaaaac atgcgggtgg cgcgtattga tgctcgtttc gacccgacca
    17701 tctatatcgc gattggtatg gcgaacttgc tggcgattgg cggcggtagt tggatggtgg
    17761 tgcagggcag tttaacgctg ggccagctca ccagttttat gatgtattta ggtctgatga
    17821 tttggccaat gctggcgctg gcatggatgt ttaacattgt ggaacgtggt agtgctgcgt
    17881 acagccgtat tcgcgcgatg ctggcggaag cgccggtggt gaacgatggt agcgaacccg
    17941 tgccggaagg gcgtggcgaa ctggatgtaa atattcacca gttcacgtat ccgcagactg
    18001 accatcctgc gctggaaaac gttaatttcg ccctgaaacc cggtcagatg ctgggtatct
    18061 gcgggccgac tggttccggc aaaagtaccc tgttgtcgct cattcagcgt catttcgacg
    18121 tcagcgaggg ggatattcgc tttcatgata ttcctctgac gaagttacaa ctcgatagct
    18181 ggcgtagccg cctggcggta gttagccaga cgccattcct tttttctgac actgtggcga
    18241 ataacatcgc gctgggttgc ccgaatgcca cccagcaaga gattgagcat gtcgcgcggt
    18301 tagccagcgt acatgacgat attttgcgtc taccgcaagg ttacgataca gaggtgggcg
    18361 agcgcggtgt gatgctttcc ggcgggcaaa aacagcgtat ctccattgct cgtgcgttat
    18421 tagtcaacgc ggaaatcctc atccttgatg atgcgctttc ggcggtggac ggacgcactg
    18481 agcaccagat cctgcataac ctgcgtcagt gggggcaggg aagaacggta atcatcagtg
    18541 cccatcgcct ttctgcactg acggaagcca gtgaaattat tgtgatgcag cacggacata
    18601 tcgcccagcg tggcaatcat gatgtgctgg cacaacaaag cggctggtat cgcgatatgt
    18661 atcgctatca acaactggag gcggcgctcg acgacgctcc ggaaaatcgc gaggaggccg
    18721 tcgatgcgta gttttagcca actgtggccg actctcaagc gcctgttagc gtacggttca
    18781 ccgtggcgta aaccgctggg gattgcggtc ctgatgatgt gggttgcggc ggcggcagaa
    18841 gtcagtgggc cgctgcttat cagctatttt atcgacaata tggtagcgaa aaataacctg
    18901 ccgttgaaag tggttgcagg gctggctgcg gcgtatgttg ggctgcaact gtttgccgcc
    18961 gggctacatt acgcgcagtc gctgctgttt aatcgggcgg cagtaggcgt agtgcaacag
    19021 ttgcgtaccg acgtgatgga tgctgcgtta cgccagccat taagcgagtt tgatacccaa
    19081 cccgtcgggc aggtgatttc ccgcgtcact aatgacactg aagtgatccg cgatctctac
    19141 gttaccgtag tggcaactgt cctgcgcagt gccgcgctgg tgggcgcgat gctggtggcg
    19201 atgttcagcc tcgactggcg aatggcactg gtggcgataa tgattttccc ggtggtgctg
    19261 gtggtaatgg tgatatacca gcgttacagc acgccgattg tccgtcgtgt gcgcgcctat
    19321 ctggcggata tcaacgacgg ctttaacgaa atcatcaatg gcatgagcgt tatccagcag
    19381 tttcgtcagc aggcgcgatt tggcgaacgt atgggggagg ccagtcgttc acactatatg
    19441 gcgaggatgc aaaccctacg cctcgacggt tttctgctgc gtccgctgct gagtctgttt
    19501 tcatcgctca ttctttgtgg cttgttgatg ctgtttggct tctccgccag cggcaccatt
    19561 gaagtgggcg tgttgtatgc gtttatcagc tatcttgggc gacttaacga acccttaatc
    19621 gaactgacca cgcagcaggc gatgctgcaa caggctgttg ttgctggtga gcgcgtgttt
    19681 gagctgatgg acggaccgcg ccagcaatat ggcaatgatg atcgcccgtt acagagcggc
    19741 accatcgaag tcgataacgt gtcatttgct tatcgcgata acaatctggt gctaaagaac
    19801 attaatctct ctgtgccttc gcgcaatttt gtggcgctgg tcgggcatac cggcagtggc
    19861 aaaagcaccc tcgccagttt attgatgggc tattacccgc taacggaagg tgagattcgc
    19921 cttgatggtc gtccattaag ttcgctaagt cacagcgcgc tgcgccaggg cgtggcaatg
    19981 gtgcagcaag atccggtggt gctggcggat accttcctcg ccaacgtgac gctggggcgg
    20041 gatatctccg aagaacgcgt ctggcaggcg ctggaaaccg tgcaactggc ggagctggcg
    20101 cgtagcatga gcgacggtat ttacacgccg ctgggcgagc aggggaataa tctctcagtt
    20161 gggcaaaagc aactgctggc actggcgcgc gtgctggtcg agacgccgca aatcctgatc
    20221 cttgatgagg caaccgccag cattgactcc ggtactgaac aggcgattca acatgctctg
    20281 gcggcggtgc gtgaacatac cacgctggta gtgattgctc accgcttatc gaccattgtt
    20341 gatgccgaca ccattctggt gcttcatcgt gggcaagccg tggagcaggg cactcaccag
    20401 caactgctgg cggcccaggg acgctactgg cagatgtatc aactgcaact tgcgggcgaa
    20461 gagctggcag ccagcgtgcg tgaagaggaa tcattgagcg cctgaatagc gcaatatttc
    20521 atcgttggtg caaaaatgta acgcactgtg cactgtcata gtgcgttttc attttcaaac
    20581 ttcttaactt cctgctctct ttctcgtttt tcatttctgg cacaccgctt gcaatacctt
    20641 cttcgtgtag cagaaccatt accgaattct gaccggaggg gatctatgaa gctggtgacc
    20701 gtgataatca aaccattcaa gctggaagac gttcgtgaag cgttatcttc cattggtatt
    20761 cagggcctga ccgtcaccga agtgaaaggt ttcgggcgtc agaaagggca tgccgagctg
    20821 taccgggggg cggaatacag cgtcaatttc ctgccaaaag taaaaattga tgtggcgatt
    20881 gctgatgacc aactcgatga agtgatcgat atcgtcagta aggcggctta caccggaaaa
    20941 attggcgacg gcaaaatctt cgtcgctgaa ttgcaacgcg tcattcgtat tcgtaccggc
    21001 gaagccgacg aagcggcgct gtaatctctg gcacacagca acaggaacga aaaatgaaga
    21061 tagcgacgat aaaaactggg cttgcttcac tggcgatgct tccgggactg gtaatggctg
    21121 cacctgcggt ggccgataaa gccgacaatg cgtttatgat gatttgtact gcgctggtgc
    21181 tgtttatgac tattccgggg attgccctgt tttacggtgg gttgattcgc ggcaaaaacg
    21241 tgctgtcgat gctgacgcag gtgacggtga catttgcact ggtctgtatt ctctgggtgg
    21301 tttacggtta ctcgctggcg tttggtgagg gcaacaactt cttcggcaac attaactggt
    21361 tgatgctgaa aaacatcgaa ctgacggcgg tgatgggcag catttatcag tatatccacg
    21421 tggcgtttca gggatcgttt gcctgcatta ccgtcggctt gatagttggg gcgctggcgg
    21481 aacgaatccg cttctcagct gtgttgattt tcgtggtggt atggctgacg ctctcttaca
    21541 ttccgattgc gcatatggtg tggggcggtg gtttgctggc ttctcacggt gcgctggatt
    21601 tcgcgggtgg caccgtggtg cacattaacg ccgcaatcgc cggtctggtg ggcgcgtatc
    21661 tgataggaaa acgcgtgggc ttcggtaaag aggcgtttaa accgcacaac ctgccgatgg
    21721 tcttcaccgg gactgccatt ctctatatcg gttggtttgg ctttaacgcc gggtcagcgg
    21781 gcacggcgaa tgaaatcgcg gcactggcat ttgtgaatac tgtggtcgca acggcggcgg
    21841 caattcttgg ctggatcttc ggtgaatggg cgctgcgtgg taagccttca ctgctggggg
    21901 cgtgttctgg cgcgattgcc ggtctggtcg gcgtgacgcc agcctgcggc tacattgggg
    21961 ttggcggcgc gttgattatc ggcgtggtag ctggtctggc gggcttgtgg ggcgttacca
    22021 tgctcaaacg cttgctgcgg gtggatgatc cctgcgatgt cttcggtgtg cacggcgttt
    22081 gtggcattgt cggctgtatc atgaccggga tttttgccgc cagctcgctg ggcggcgtgg
    22141 gcttcgctga aggtgtgacg atgggccatc agttgctggt acagctggaa agcatcgcca
    22201 ttacgatcgt ctggtccggt gttgtggcat ttatcggcta caaattggcg gatctgacgg
    22261 ttggtctgcg tgtaccggaa gagcaggagc gagaagggct ggatgtcaac agccacggcg
    22321 agaatgccta taacgcgtaa caagcactgc aaaaaacagc cggacggttt tcacctccgg
    22381 ctattttttt aattgtgatt acgcatcacc ccttcctgaa cggtcgaggc aaccagtacg
    22441 ccgtcttggg tataaaactc accgcgcaca aagccacgtg cgctggacgc cgaggtgctc
    22501 tccacgctat acagcagcca ttcattcaaa ttaaacgggc gatggaacca catggaatgg
    22561 tcaatggtgg caatctgaat ccccggttcg agaaaaccga tgccgtgcgg ctgtagagct
    22621 accggcagga agttaagatc agaagcgtaa ccgagcagat actgatgaac gcgcaggtca
    22681 tccggcacgc taccatttgc gcggatccac acctgacgat gtggttctgc gacgtgacct
    22741 ttcagtgggt tatgaaactc caccggacgg acttccagcg gacgatcgca gatgaattta
    22801 tctttcagca ctggcggcag caggtgcgcc agcgattggg cgatttgcgt ttccgaaggt
    22861 aggccatcag gcgctggcgc ggacggcatt gttttttgat gttcgaaacc cgcttctggt
    22921 gcctggaaag aggcagtcat ataaaaaatc ggtttgccgt tttgaatagc agcaacccgg
    22981 cgggcgctga agctgttacc gtcacgcagc gtttcgacat cataaataat cggcttctta
    23041 ctatcgccag ggcgaagaaa gtagctgtga aacgaatgta ccagccgctc ttcagggacg
    23101 gtctcttttg cagcatacaa ggcctgaccc acgacctggc cgccaaacac ctggcgtaaa
    23161 cctaaatctt cactctggcc gcgaaagagt ccttcctcaa ttttttccag atttaacaat
    23221 gtcagtaaat tttttagcgc ctgactcata taactctcca gtaacaaagc tgccgcagca
    23281 agccaaagtg agttgagtat aacgcaaatt tgctactggt ccgatgggtg caatggtctg
    23341 aattacgggc taattacagg cagaaatgcg tgatgtgtgc cacacttgtt gacgttacta
    23401 ttttgttaac cactcttccg gcgaggaaag ttagcccgct ggtgcattga taataaggag
    23461 aaatgaatga aactcgtgca catggccagt ggtttagcgg ttgcgattgc gttggcggct
    23521 tgcgcagata aaagcgcgga tattcagacg ccagcaccgg ctgcaaatac gtctatttca
    23581 gcaacacaac aaccagctat ccagcaaccg aatgtctccg gtaccgtctg gatccgtcag
    23641 aaagtcgcac tgccgcctga tgctgtgctg accgtgacac tttctgacgc gtcgttagcc
    23701 gatgcaccgt caaaagtgct ggcgcagaaa gcggtgcgta ctgaaggtaa acagtcacca
    23761 ttcagctttg ttctgccatt taacccggca gatgttcagc cgaacgcgcg tattctgttg
    23821 agtgcggcga ttaccgtgaa tgacaaactg gtatttatca ccgataccgt tcagccggtg
    23881 atcaaccagg gcggaactaa agccgacctg acattggtgc cggtacagca aaccgccgtg
    23941 ccggttcagg ccagcggtgg cgcaacgact accgtacctt cgacttcacc aactcaggtg
    24001 aatccgtctt cggcagttcc agctcctacg caatattaag cgacgttaac cctctccgcc
    24061 cggagagggt tagtagttcc agcgataacg ctgcaagtcg atttgcccgc ttcccgatac
    24121 catcacacct tctgccagta atgcctgtcg ctgacgctgt aaatccggtc cggttagcga
    24181 aattgtgccg tggcgattaa ccacccggtg ccagggtaag gtgctgcctt cagggagacg
    24241 ctttaacaca ccgcccacct ggcgcgcggc gcggggcgat cccgccagtt tcgccacatc
    24301 accgtaagtg gtgacatagc cttcgggaat agcggcgacg atttgccaga cgcgttgggg
    24361 aaatgaatct tctttttcca tcttttcttc ctgaggtaat ttttcagcat aatctggaaa
    24421 aacgcccgag tgaagtcgca ttgcgcaaga aaccagcatc tggcacgcga tgggttgcaa
    24481 ttagccgggg cagcagtgat aatgcgcctg cgcgttggtt ctcaacgctc tcaatggggg
    24541 ctctgttggt tctcccgcaa cgctactctg tttaccaggt caggtccgga aggaagcagc
    24601 caaggcagat gacgcgtgtg ccgggatgta gctggcaggg cccccaccca tttctgcctc
    24661 ccaccgtttc gtcaaaaaat cccaacatgg ctaaacttta accacaactg acgtcgcaag
    24721 aattgtctgg ctgcgcagta cgcttcggag gtatgtctga tgaagtatgt tgatggtttt
    24781 gtggttgccg ttcctgccga taaaaaggat gcctatcggg aaatggccgc taaggctgcg
    24841 ccattgttta aagagtttgg cgcgcttcgt attgtcgaat gctgggccag cgatgtaccg
    24901 gatggcaaag tgaccgattt tcgtatggcg gtgaaagcgg aagagaatga agaggttgtc
    24961 tttagctgga ttgaataccc ttcaaaagag gtccgcgacg ctgctaatca aaagatgatg
    25021 tcggacccac ggatgaaaga gttcggcgag tccatgccgt ttgacggcaa gcgaatgatc
    25081 tatggcggat tcgagtcaat catcgacgaa taggtagcgt gacgggctgt gcggcgtgcc
    25141 gtcagcccaa aaattcacaa atgttgctcg gcccagcgta agaaatcttc tttcggtaat
    25201 gccttgctgt agagccagcc ctgaccgtag tgcacgccat gctggcgtaa ccactcttct
    25261 tgtttactgg tttcgattcc ctccgctacc attttcagtt tcagtgtttt tgccatttcg
    25321 atgatatgcg gcgtgacatt tttatattcc agcgcatcaa cgaaagattt atcgatcttc
    25381 agaatgtcga catccagatc ctgtaaataa cttaaacttg aataccccgt accaaaatca
    25441 tcaagataaa tttcatggcc cgcctcccgg tagcgagaaa ttatcggggc gctggttttc
    25501 ggatcggcaa actcgcgttc agtgagttca agcgcgatct gtctgggatt aacctgatag
    25561 tgattgatca tgtcacgcag caattgcggg attttttccg aggtgagcac ggtggattca
    25621 agattgatcg aaatatgctg ctgtggatgc tgacgcagcc agtcgcccat atcttcaaag
    25681 acgcttctta taatcagtag cgtcaatggc tcagaaaggc ccgtttgctg tgccagcgga
    25741 ataaaactat ctggtgacaa ccaactaccg tctgtctgcg gccagcgcgc cagtgcctca
    25801 gcaccgacaa ttttgccatt ggctaaggag acaatcggct gatagtgcac gcaaatatca
    25861 cgattttcga tagcatcctg cagccgatga tgcggtgact gaatacggcg caaaatacgc
    25921 agcacaaaca tcgctgccag caggccaatc accaacccga gcggtaacca aataaagact
    25981 tgccgatgcc agcctttctg caacattttc gttgaagccc atgtgatgat cgaaatattc
    26041 atctccggta agggcaggat atcgtaaatg attccattat tttcgatatg ctcaccgggt
    26101 gttttttgta gcctggtaat aattccctga gcaatttcat cgctgctggt tatgacaacg
    26161 ttatgggcat tgccaataat ggcggcatca atttgccatg agctataggg aatgacatca
    26221 ataaaggaag cggggtcgat catgacgaca taatgtgccg ttcccatggc gaccatgtaa
    26281 cggataatgc ctaaatcgtt atgcgatgtt aaccagacac gataaccatc tttcgaaatt
    26341 ttacctggct cggggaaggt atcgggcgga ctttcatgct ccagagacga acactgggga
    26401 acgttgttat cgatataagc cacttcctga atatagcgat aactgtaaga tacccgacgc
    26461 atttccatga gatgggcttc gctacaggca gcgccttgcc atctttccag ctcctgcagc
    26521 gcatctttcc cttgtgtcgc caccttattg gctcgaatag cgacgcggga ggaataggta
    26581 tccagctctt caataaacga tgtttctacc tgctgatggg ccagccagat gcttaagccg
    26641 acaggcagca atactgaaag aatcagtact cccgaaataa ggccgaccag atgtcgtgtt
    26701 ctcaccagca tatccttatt aacacagtgt agggaaagta tatctgattg tggaaaaagg
    26761 ctggctggag ataaggaggt agagctggta tttcatgaag tgatgggtgg aaacgatcat
    26821 tgtcatttga ccaggcgcag agctttacgc ctggtatgta accctaactt aagatgttga
    26881 ttcggcgcga gcgagggtaa aaatatcgta aaaagacagt tcacctttac ggacgatcat
    26941 tttttgcagt tgttccttat gaacatccgc gacacgcgga gagttcaaga tggcttcaac
    27001 cagcgcggca ggtacaaagc gcgtgttgta ccattcgccg ttatagcaaa cgcggaaatc
    27061 cagcacgtcg atatcttcat agcggtagcg tgggtagaca tcgaaaaaga agaaaccatt
    27121 aagcaggata aataatacaa ccagtagcca gacagagcct gacagcgttt cggactgcaa
    27181 catcaccgcc agggtggcaa aaaaagcaac gtacataccg ataaacagcc ccggatgttt
    27241 acggataaaa ctgatactaa agcggggttt gttgtcgcgt ttttcgcggg tattcagaga
    27301 ctcaatcgtt tcggtcagca ggcgttgtat ttcggtcatc gtttgccttc gtggtatctg
    27361 caactttcca gggaacaccc tattttaacg tgggaatagt agaggggaag taacagatct
    27421 accacaattt tgcataacag ttgcgaaaaa ccgattacaa ttttttaatt attctggctg
    27481 gattaccgcc cacgacaacg ttgtccggga catcttttgt gacaactgca cctgaggcta
    27541 ccacgacgtt atcaccaatg gtcacaccag ggttaatgac cgcgcgtccg ccaatccaga
    27601 cgttattacc gatggtgacg ggtttcccca gttcagcacc gctattacgt gctacagggt
    27661 cgatgggatg tgttgccgtg tagatatgaa cgcctggtgc caacatacag ttatcaccga
    27721 tgcgaatagg gcagacatca agcatcacgc aatcgaagtt ggcgaaaaaa ttattaccga
    27781 gaaaaatgtt atagccatag tcacagcgaa acgttggctc aatataagcc tctgtcacct
    27841 gaccgaatag atcagcgaga atttgctggc gtaatgtgtg ctcttccgcc agggaatgat
    27901 tgtatcggtg aataagctga cgagcgcgca ggcgatcgcg agataacgtc tcatctgccg
    27961 agcgatacaa ctcaccagca atcatctttt ctttttctgt gctcattact gacctccggt
    28021 tgctgaaaat gcggcaacac agtaagacga aaagtcagtg aagagaacgt tcccattaca
    28081 tctgtgtgat aaagatcaca tagggtatgt cttcatggcg aaaaagtata aaattcttaa
    28141 taaacagccg gttatagctc cgaaagcgaa ttatttatta gcgaataaat ttccaaactg
    28201 aggaagggat cttgtcgtac agtttattca tggtcaattc ggcgaggcgg tgatctgcgg
    28261 ctgagtaaaa taccgccagt tcattatctg ataattcgta tttatttttc tcgataacac
    28321 gctccagcgt gtcaattgtc tggcaacgac gtaaacgcat taaataatcg gttttcgtta
    28381 aaggtttttc ggacatactt ctacccatgg ttgtaataat tctaacaaga taaactcgca
    28441 ggattctctt tcgtcgcatt gacaaaacaa cggaatagtc gattacctga cttccgccat
    28501 ttctgaagat cctgcatatt aataccataa ctactgaaca acataaaggt gtcatccaga
    28561 tattcgtcga tctgctcaat gagcttattg tcttcattat acttaatttt gtaattaagt
    28621 gcgaaggtcg caatatgctc aatcagttca ttcaactgga ggttgatcgc cgaggttggg
    28681 tcgttaaccc agccatgatt gctttcttca aggtttgcaa ggcagtcatg atacagggtt
    28741 tcacagagaa acttaagctg tgcgatatca tgtcttttgg gtgagtattc atccataacg
    28801 cgtccccttc ttagcggttg aactaacgga cacctttcgg gatggaaaaa acttactgac
    28861 ctggacttgc cctctcgttt gttagcttaa ctataagcca ctctttgcag gttttcatcg
    28921 cattattacg aaaaattaca attaggaagg aagcgggaca ggactcactt tttcatacta
    28981 gcaccaaccc gcagtaggta aaagcagttt gctcgaacag tttataacat ttcaggatta
    29041 tcgccagatg acattggtga cactaatacc aggattgctc tgaatatgac gtaataaccg
    29101 aggaatgaat aaagaattac cgcaaataat taagaatagc ctctaaatga ttatggatta
    29161 ttaaatctat tagcgctact taatataaat taactaacaa tcagtatttt ttatgaattt
    29221 tatccgtggt taatactggt tttcgtatga gatcctgagt tggtggttca attactcctt
    29281 aatgttcgta ggttatgcat aaaaaaggcc gcttgcgcgg ccttagtgat tacacgttgt
    29341 atcaatgatg atcgacagta tggctgtgct cgatatcttc attcttgcgg ctaaagcggc
    29401 ggcgaaccac cacaaagaat accggaacga agaagattgc cagtaccgtt gcggtcacca
    29461 tcccgcccat tacaccggta cctactgcgt tctgcgcgcc ggaaccagca ccagtactga
    29521 taaccagcgg cataacgccg aggataaacg ccagcgaggt catcaggatc ggacgtaaac
    29581 gcatccgcac cgcatcaagc gtcgcttcaa tcagaccttt accttcttta tccatcaagt
    29641 ctttggcgaa ttcgacgata agtatcgcgt tcttcgccga caacccaatg gttgtgagca
    29701 ggcctacctg gaagtaaacg tcattggtca ggccacggaa ggtggcagcc agcaacgcac
    29761 cgataacccc cagcggaacg accagcataa cggagaacgg aatcgaccag ctctcgtaca
    29821 gcgccgccag acacaggaac acgacaatca acgaaatcgc gtacagtgaa ggtgcctggt
    29881 tgccggagag acgttcctga taggacatcc ccgtccagtc atagccaaca ccggtaggca
    29941 gtttgctcgc cagttgttcc atcagctcca ttgcttcacc ggtactttta cccggtgccg
    30001 cctggcctaa gatttccatg gatggcaggc cgttgtaacg ttccagacgc ggcgaaccgt
    30061 actcccaacg agaagaggag aacgccgaga atggcaccat ctgaccatca gcagcacgaa
    30121 cataccagtc gccgatatca tccggcagca tacggtattt cgcttctgac atgacgtaaa
    30181 ctttcttcac acgaccgcgg tcgataaagt cgttcacata gctgccgccc catgcagcgc
    30241 ccagagtggt gttaatgtcg ttgatagaaa cacccagcgc ctgcgctttt tcctggtcga
    30301 tatcaatctt aaactgcggg gtatcttcca gaccgtttgg acgtacgctg gtcaacatat
    30361 cagggtgctt cgctgcttct gcaagcagct ggttacgcgc ctgagtcagt ttttcgtgac
    30421 caaggccagc ctggtcaatc agctcaaagt caaagccggt tgcggtaccc agttccacga
    30481 ttgcgggcag gttaaaggcg aaaaccatcg catctttgat ttgcgagaaa gcgcgtgttg
    30541 cacgcatggt aatcgcttca actttgtttt cttcgcccgg acgatcggcc cagtccttca
    30601 aggaaacgaa cgcaataccg gtattctgac cacgtcccgc aaagccgaag ccgttaacgg
    30661 cgaacaccga ctcaacgttg ttcttttctt tggtcagata gtaatgcgtt acctcattga
    30721 gcactttctg tgtacgttcc tgcgttgcac ctgctggcag ctgaaccatg gtcataaata
    30781 cgccctggtc ctcatctggc aagaaggagc ttggcagacg cacaaacaga taggccatgc
    30841 cgaccacgat gatcagatac agcaccaggt aacgccctgt actgcgcaga ataccgccta
    30901 cgctgtcggt gtagtggtgc gtgctcttct cgaacatgcg gttaaaccag ccgaagaagc
    30961 cttttttacc ttccccgtga tcgcctttgg caatcggttt cagcatggtg gcacaaagag
    31021 ccggagtcag gatcaacgct accagtaccg acagcgccat tgctgaaaca atggtaatag
    31081 agaactgacg atagatagca ccagtagaac cgccaaagaa ggccatcggt acgaataccg
    31141 ccgacagtac catcgcgata ccgaccagag cgccctgaat ctgccccatc gacttacggg
    31201 tagcttcttt tggcggcaaa ccttcttccg ccataacacg ctcaacgttt tctaccacaa
    31261 cgatagcgtc atccaccaac aagccgatgg cgagcaccat cccgaacatt gttagcgtgt
    31321 ttatcgagaa gccaaaggcg gcaaggacgg caaaggtccc gagcaatacc accggtacgg
    31381 caatggtcgg aatcaacgtc gcgcggaagt tctgcaggaa cagatacata accaggaaca
    31441 cgaggatgat cgcttcgacc agcgttttaa ccacttcgtg aatagagatt ttcacgaacg
    31501 gcgtggtgtc gtacgggtaa acaattttca gacctgacgg gaagaacggt tccatcttcg
    31561 ccagttcagc acggattgcc gcagcggtat ccagcgcgtt tgcaccggtc gccagcttga
    31621 tccccagacc ggaagccggt tggccgttaa actctgcgat gatgtcgtag ttctcaccac
    31681 ccagctcaat cttcgcgacg tcacgcagca gcacgcggga accatcctga ttcactttca
    31741 gcaggatttt gccgaactct tcagtagagg tcagacgcgt ctgagcaata atagaggcgt
    31801 taagctgttg gcctttcacc ggcggcgtac caccgagctg acccgccgca acctgggcgt
    31861 tctgcgcttt gatggcggta atgacatcaa ccggcgttag ctggaatttg ttcagctcat
    31921 tcgggttcat ccagatacgc atcgcgtact gtgaaccgaa caactgaaca tcacccacgc
    31981 ccgacgtacg gctgatggca tctttcatat tcgccgccac gtagtcggag atatcctcct
    32041 gcgtcatggt gccatcggtg ttgataacgc cgacaaccat caggaagctg ctggatgatt
    32101 tctcaacgct caccccttgc tgctgaactt cttgcggcag caacggcatc gccagctgca
    32161 gtttgttctg cacctgaacc tgcgcgatat ccgcatcagt accagactca aaggtcaggg
    32221 tgatctgcac ggtacccgtg gagtcactgt tagaggacat gtacatcagg ttatcgatac
    32281 cgttcatatt ctgttcgata acctgtgtca ccgtgtcctg cactgttttc gcatcagcgc
    32341 cggggtagga ggcggagatc gttactgccg gcggtgcaat cgtaggatat tgcgccaccg
    32401 gcagtttgag gatcgccagc ccccctgcca acatgatgat aatggcgatc acccacgcaa
    32461 aaatcgggcg atcgataaag aaattaggca tgtcttaacg gctcctgttt aagttaagac
    32521 ttggactgtt caggctgagc accgcttgcg gcttgctggt tattatcagc ggtaacttct
    32581 tgtgctttta cctggacacc aggacgcact ttctgcagcc cacttattac tacgcgatcg
    32641 cctgctttca gaccttctgt caccagccac ttatcgccaa tagcctggct tgcaacgatc
    32701 ggacgggtct ccactttgtc atccgcgcca actaccagta cggtggcatc gccacgcggc
    32761 gtacgggtta cgccctgttg cgggactaaa atagcgtttg gattaagccc ttcttccaga
    32821 cgtgcgcgca cgaacatacc cggcagcaga gtgtgatccg ggttcgggaa gatagcgcgt
    32881 agggtgatag acccagtggt ctgatcaacg gtaacgtcag agaattccag cgtaccgtcc
    32941 tgcgggaact taatgccgtc actggtgatc agcgacactt tggctttgcc gttctcttgt
    33001 ttcagcgtgc cattcgccag ttcctgtttc aggcgcagga agtcgttgct ggactgggtc
    33061 acatcaacgt agatcggatc aagttgctgc acggttgcca gcgcagtcgc ctgaccgttc
    33121 tgtaccaatg cgccttccgt cacgttcgac ttaccaatgc gaccgctaat cggagaggtg
    33181 actttggtgt aagccagatt gattcgcgca gtttcaacgg cagctttcgc cgcagttacc
    33241 gcagcattcg cctgttgcgc atcagccaga gcctgatcgt actcttgctt actgatgtac
    33301 tgagtaccga gcagtttctg ataacgattc accgtcaatt gcgcgatatt ggctgcagcc
    33361 tgggctttcg ccagatcacc tttcgcactg tcgtatgtcg cctgataggt cgcaggatca
    33421 atctgataga gagagacacc tgcttcgatg tcgctacctt ctttgaaatt acgcttcagg
    33481 ataatcccgc taacttgagg acgaacttct gcgatccggt aggcactggt gcgacccgga
    33541 agctcggttg tgatctgcag aggttcagtt ttgactgtta ctacgccaac ggcgggcatc
    33601 tgctggccac cttgttgggc ctgtttgtcg tcacatcctg ttagggctaa gctgcctgag
    33661 agcatcagaa cgaccgccag aggcgtaaac cctctgtttt tgttcatatg taaacctcga
    33721 gtgtccgatt tcaaattggt caatggtcaa aagttaataa acccattgct gcgtttatat
    33781 tatcgtcgtg ctatggtaca tacattcaca aatgtatgta aatctaacgc ctgtaaattc
    33841 acgaacatat ggcacgaaaa accaaacaag aagcgcaaga aacgcgccaa cacatcctcg
    33901 atgtggctct acgtcttttc tcacagcagg gggtatcatc cacctcgctg ggcgagattg
    33961 caaaagcagc tggcgttacg cgcggtgcaa tctactggca ttttaaagac aagtcggatt
    34021 tgttcagtga gatctgggaa ctgtcagaat ccaatattgg tgaactagag cttgagtatc
    34081 aggcaaaatt ccctggcgat ccactctcag tattaagaga gatattaatt catgttcttg
    34141 aatccacggt gacagaagaa cggcgtcgat tattgatgga gattatattc cacaaatgcg
    34201 aatttgtcgg agaaatggct gttgtgcaac aggcacaacg taatctctgt ctggaaagtt
    34261 atgaccgtat agaacaaacg ttaaaacatt gtattgaagc gaaaatgttg cctgcggatt
    34321 taatgacgcg tcgcgcagca attattatgc gcggctatat ttccggcctg atggaaaact
    34381 ggctctttgc cccgcaatct tttgatctta aaaaagaagc ccgcgattac gtcgccatct
    34441 tactggagat gtatctcctg tgccccacgc ttcgtaatcc tgccactaac gaataaccct
    34501 gaatctgact ccaggatttt tcctggacat tttcgtcgtt gctattctgg ttcactgcgt
    34561 cgtgatattc ttgcggtttg actttttcag gtcgttcttc aggttcagaa accttcattc
    34621 atcatgacta tgttccagta ttacaaacga tcacggcatt ttgttttttc agcatttatt
    34681 gcttttgttt ttgtcttgtt atgccagaac acggcgtttg cgcgggcgtc atcgaatggt
    34741 gatctgccga caaaagcgga cctgcaggcg caacttgact cactaaataa acaaaaagat
    34801 ctttctgctc aggacaaact ggtgcagcag gatctgacag atacattagc caccctcgat
    34861 aaaatcgatc gcataaaaga agagacagtt cagctacggc aaaaagtcgc tgaagcgccg
    34921 gaaaaaatgc gccaggcgac cgcggcgtta acagcactta gcgatgtcga taacgacgaa
    34981 gaaacgcgca aaattctgag cacgctttcg ttgcgccagc tggaaactcg cgttgcccag
    35041 gcgctggacg atttgcaaaa cgcacaaaac gatctggcgt cttataacag ccagctggtt
    35101 tcgttacaga cgcagcccga acgcgtgcaa aatgcgatgt ataacgcttc gcagcagctg
    35161 caacaaattc gcagtcgtct ggatgggact gatgtcggcg agacagcctt acgtcccagc
    35221 cagaaagtgt taatgcaggc ccagcaggcg ttgctgaatg cggagattga ccagcagcgt
    35281 aaaagcctgg aagggaacac cgtcttgcag gataccttgc aaaagcaacg tgattacgtg
    35341 acggcgaaca gcgctcgtct ggagcaccag ttacaactgt tgcaagaagc ggtaaacagc
    35401 aagcgcctga ctttaaccga aaaaacggcg caggaagccg tctccccgga tgaagccgcg
    35461 cgtattcagg ctaatccgct ggtgaagcag gaactggaaa ttaaccagca gttaagtcag
    35521 cgtctgatta ccgcgactga aaacggtaat cagttgatgc agcaaaacat taaagtcaaa
    35581 aactggctgg agcgggcgct gcaatcggaa cgcaatatta aagagcagat tgccgtcctg
    35641 aagggcagcc tgctattgtc tcgtatcctt taccagcaac aacaaacgct gccctcggcg
    35701 gatgaactgg aaaacatgac caaccgcatc gcggatttgc gtctcgaaca gtttgaagtt
    35761 aaccagcagc gtgatgcact cttccagagc gatgcgttcg tcaacaaact ggaagaaggt
    35821 cacaccaacg aagtcaacag cgaagttcac gatgcgttat tgcaagtggt tgatatgcgt
    35881 cgcgaattgc tggatcaact caacaaacag ttgggtaacc agctgatgat ggccattaac
    35941 ctgcaaatca accagcagca gttaatgagt gtgtcgaaaa acctgaaatc catcctgact
    36001 cagcaaatct tttgggtgaa cagtaaccgt ccaatggact gggactggat caaagcgttc
    36061 ccgcaaagcc tgaaagatga atttaagtcg atgaaaatca cggtgaactg gcaaaaagcc
    36121 tggcccgccg tttttatcgc tttcctcgct ggtttgccgc tgctgttgat tgccgggctg
    36181 atccactggc gtctgggctg gctgaaagcg tatcaacaaa aactggcttc cgctgtgggt
    36241 tccctgcgta acgacagcca gctcaacaca ccaaaagcga tccttatcga cctgatccgt
    36301 gcgctgccgg tgtgcctgat tattctcgcg gttggcctga ttctgttgac catgcagctc
    36361 aacatcagcg aactgctatg gtcgttcagc aaaaaactgg cgatattctg gctggtgttt
    36421 ggcctgtgct ggaaggtact ggagaaaaac ggcgttgccg tacgtcactt cggcatgccg
    36481 gaacagcaga ccagccactg gcgtcggcaa attgtccgca tcagtctcgc attgctgcct
    36541 atccatttct ggtctgtggt ggcagaactt tccccgctgc atctgatgga tgatgtgctg
    36601 gggcaagcga tgattttctt caacctgctg ctgattgcct tcctggtatg gccgatgtgc
    36661 cgcgaaagct ggcgtgataa agagtcgcac accatgcgac tggtcaccat taccgtgctg
    36721 tcgataatcc cgattgcgct gatggtgctg actgctacag gctacttcta cactacgctg
    36781 cgtctggcag gacgctggat tgaaaccgtt tatctggtga tcatctggaa cctgctgtac
    36841 cagacggtac tgcgtggctt aagcgtagcg gcgcggcgta tcgcctggcg tcgtgcgctg
    36901 gcgcgtcggc agaatctggt gaaagagggc gcagaaggtg ctgaaccgcc ggaagaaccc
    36961 accattgcac tggagcaagt taaccagcag acgctgcgta ttaccatgtt gctgatgttt
    37021 gcgctgttcg gtgtcatgtt ctgggcaatt tggtccgatt tgatcaccgt gttcagctat
    37081 ctcgacagca tcacgctctg gcattacaac ggcactgaag ctggcgctgc ggtggtgaaa
    37141 aacgtcacca tgggcagtct gttgtttgcg attatcgcct caatggtggc ctgggcgttg
    37201 attcgcaacc tgcctggttt actggaagtg ctggtgctct cgcgactgaa tatgcgccag
    37261 ggcgcgtcgt atgccattac taccatcctt aactacatca ttattgctgt tggtgcgatg
    37321 acggtgttcg gatcgctggg cgtctcttgg gataaactcc agtggctggc cgcagcatta
    37381 tccgtaggtc ttggttttgg tttacaagaa attttcggta acttcgtctc cggtttgatc
    37441 attctattcg aacgtccggt gcgtattggc gatacggtaa ccattggtag cttctcgggg
    37501 acggtaagta agatccgtat tcgtgcgaca acgattaccg atttcgatcg caaagaagtg
    37561 atcatcccga acaaagcgtt tgttaccgag cgtctgatca actggtcgtt gactgacact
    37621 actacgcgtc tggtgatccg tctcggcgtg gcctatggct ccgatctgga aaaagtgcgt
    37681 aaagtgttac tgaaggcggc gactgagcac ccaagagtga tgcacgaacc aatgccggaa
    37741 gtcttcttta cggcgtttgg tgccagcacg ttggatcatg agctgcgtct gtatgtgcgt
    37801 gaactgcgtg accgtagtcg tactgtcgat gagctgaacc gtactatcga tcagctgtgc
    37861 cgtgaaaacg acatcaacat tgcctttaac cagcttgaag tgcatctgca caacgagaag
    37921 ggcgatgagg tgacggaagt aaaacgcgac tacaaaggcg acgatccgac accagcggtt
    37981 gggtaataac caaagggggc gacatttagt tgccccgaga ttactgacaa agtgcgcgtt
    38041 gttcatgccg gatgcggcgt gaccgcctta tccggcctac gaaaccgcaa gaattcaata
    38101 tattgcagga gcggtgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgccc
    38161 acaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgc
    38221 gcgttaattt cttttcataa tcgcgtttta caatatccag cgcgcgcagc acggtgcggg
    38281 ctgggatctg attttcttcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
    38341 gggcattttc aagtgacata ctctctccat tgctagcggg ttaagcgcgc tagcctgttt
    38401 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
    38461 cgatgcagcg tttgctgttc cacgagatcg gtcacccgca ccaggcgggc tctgcgttcg
    38521 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
    38581 cgggcaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
    38641 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
    38701 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcgtc gagacaagcc
    38761 tgtagtgttg tcgcacgagt ctgaaaaaga tgcctgtcga aacgagcact tagcgtggcg
    38821 aa
//
LOCUS       DAOFQD010000030        38695 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.030, whole genome shotgun sequence.
ACCESSION   DAOFQD010000030 DAOFQD010000000
VERSION     DAOFQD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.030"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            311..1177
                     /gene="folD"
                     /locus_tag="QBF46_001644"
     CDS             311..1177
                     /gene="folD"
                     /locus_tag="QBF46_001644"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDQ6673836.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1179..1391
                     /gene="ybcJ"
                     /locus_tag="QBF46_001645"
     CDS             1179..1391
                     /gene="ybcJ"
                     /locus_tag="QBF46_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDQ6673837.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1499..2020
                     /locus_tag="QBF46_001646"
     CDS             1499..2020
                     /locus_tag="QBF46_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ6673838.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2056..3441)
                     /gene="cysS"
                     /locus_tag="QBF46_001647"
     CDS             complement(2056..3441)
                     /gene="cysS"
                     /locus_tag="QBF46_001647"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDQ6673839.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3615..4109
                     /gene="ppiB"
                     /locus_tag="QBF46_001648"
     CDS             3615..4109
                     /gene="ppiB"
                     /locus_tag="QBF46_001648"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDQ6673840.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4112..4834
                     /gene="lpxH"
                     /locus_tag="QBF46_001649"
     CDS             4112..4834
                     /gene="lpxH"
                     /locus_tag="QBF46_001649"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDQ6673841.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4952..5461
                     /gene="purE"
                     /locus_tag="QBF46_001650"
     CDS             4952..5461
                     /gene="purE"
                     /locus_tag="QBF46_001650"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDQ6673842.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5458..6525
                     /gene="purK"
                     /locus_tag="QBF46_001651"
     CDS             5458..6525
                     /gene="purK"
                     /locus_tag="QBF46_001651"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDQ6673843.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFG"
     gene            complement(6720..7613)
                     /locus_tag="QBF46_001652"
     CDS             complement(6720..7613)
                     /locus_tag="QBF46_001652"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDQ6673844.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVMAVSGYVRSRGKPAW
                     IGALSRIEETLAGKAGTCISL"
     gene            complement(7610..8425)
                     /locus_tag="QBF46_001653"
     CDS             complement(7610..8425)
                     /locus_tag="QBF46_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDQ6673845.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFAYSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8436..9695)
                     /locus_tag="QBF46_001654"
     CDS             complement(8436..9695)
                     /locus_tag="QBF46_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDQ6673846.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9705..11372)
                     /locus_tag="QBF46_001655"
     CDS             complement(9705..11372)
                     /locus_tag="QBF46_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDQ6673847.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIAQAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIAKLEDAGIAVVSSLPEATLLAAALIYPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11690..12739
                     /gene="allD"
                     /locus_tag="QBF46_001656"
     CDS             11690..12739
                     /gene="allD"
                     /locus_tag="QBF46_001656"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDQ6673848.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGVPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIDINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12761..13996
                     /gene="allC"
                     /locus_tag="QBF46_001657"
     CDS             12761..13996
                     /gene="allC"
                     /locus_tag="QBF46_001657"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDQ6673849.1"
                     /translation="MITHFRQAIEEALPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNIYDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKNMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            14007..14792
                     /gene="allE"
                     /locus_tag="QBF46_001658"
     CDS             14007..14792
                     /gene="allE"
                     /locus_tag="QBF46_001658"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDQ6673850.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVITGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSSNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14920..16065)
                     /gene="glxK"
                     /locus_tag="QBF46_001659"
     CDS             complement(14920..16065)
                     /gene="glxK"
                     /locus_tag="QBF46_001659"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDQ6673851.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16087..17388)
                     /locus_tag="QBF46_001660"
     CDS             complement(16087..17388)
                     /locus_tag="QBF46_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDQ6673852.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWFFCFPSSH
                     SLSGELHWQWFPLGSDGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTQLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17445..18806)
                     /gene="allB"
                     /locus_tag="QBF46_001661"
     CDS             complement(17445..18806)
                     /gene="allB"
                     /locus_tag="QBF46_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDQ6673853.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18866..20320)
                     /locus_tag="QBF46_001662"
     CDS             complement(18866..20320)
                     /locus_tag="QBF46_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDQ6673854.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVVYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20489..21367)
                     /gene="glxR"
                     /locus_tag="QBF46_001663"
     CDS             complement(20489..21367)
                     /gene="glxR"
                     /locus_tag="QBF46_001663"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDQ6673855.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKTLALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21467..22243)
                     /gene="hyi"
                     /locus_tag="QBF46_001664"
     CDS             complement(21467..22243)
                     /gene="hyi"
                     /locus_tag="QBF46_001664"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDQ6673856.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22256..24037)
                     /gene="gcl"
                     /locus_tag="QBF46_001665"
     CDS             complement(22256..24037)
                     /gene="gcl"
                     /locus_tag="QBF46_001665"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDQ6673857.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRAMAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24127..24942)
                     /gene="allR"
                     /locus_tag="QBF46_001666"
     CDS             complement(24127..24942)
                     /gene="allR"
                     /locus_tag="QBF46_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDQ6673858.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(25020..25502)
                     /gene="allA"
                     /locus_tag="QBF46_001667"
     CDS             complement(25020..25502)
                     /gene="allA"
                     /locus_tag="QBF46_001667"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDQ6673859.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25732..26658
                     /gene="allS"
                     /locus_tag="QBF46_001668"
     CDS             25732..26658
                     /gene="allS"
                     /locus_tag="QBF46_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDQ6673860.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26727..27821
                     /gene="mnmH"
                     /locus_tag="QBF46_001669"
     CDS             26727..27821
                     /gene="mnmH"
                     /locus_tag="QBF46_001669"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDQ6673861.1"
                     /translation="MQERHTEQNYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQAIIELSQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(28534..30948)
                     /locus_tag="QBF46_001670"
     CDS             complement(28534..30948)
                     /locus_tag="QBF46_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6673862.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENMLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(30945..31631)
                     /locus_tag="QBF46_001671"
     CDS             complement(30945..31631)
                     /locus_tag="QBF46_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6673863.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDCCLR
                     LVNGQLQEEA"
     gene            31602..32225
                     /gene="tesA"
                     /locus_tag="QBF46_001672"
     CDS             31602..32225
                     /gene="tesA"
                     /locus_tag="QBF46_001672"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDQ6673864.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            32215..33024
                     /gene="ybbO"
                     /locus_tag="QBF46_001673"
     CDS             32215..33024
                     /gene="ybbO"
                     /locus_tag="QBF46_001673"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDQ6673865.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKIRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            33085..33939
                     /gene="cnoX"
                     /locus_tag="QBF46_001674"
     CDS             33085..33939
                     /gene="cnoX"
                     /locus_tag="QBF46_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDQ6673866.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(34002..34781)
                     /gene="fetB"
                     /locus_tag="QBF46_001675"
     CDS             complement(34002..34781)
                     /gene="fetB"
                     /locus_tag="QBF46_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDQ6673867.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(34768..35445)
                     /gene="fetA"
                     /locus_tag="QBF46_001676"
     CDS             complement(34768..35445)
                     /gene="fetA"
                     /locus_tag="QBF46_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDQ6673868.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKDIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35591..36508
                     /locus_tag="QBF46_001677"
     CDS             35591..36508
                     /locus_tag="QBF46_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDQ6673869.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            36505..36963
                     /locus_tag="QBF46_001678"
     CDS             36505..36963
                     /locus_tag="QBF46_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDQ6673870.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(36964..37371)
                     /gene="cueR"
                     /locus_tag="QBF46_001679"
     CDS             complement(36964..37371)
                     /gene="cueR"
                     /locus_tag="QBF46_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDQ6673871.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(37496..>38695)
                     /locus_tag="QBF46_001680"
     CDS             complement(37496..>38695)
                     /locus_tag="QBF46_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6673872.1"
                     /translation="FALLGQAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNG
                     GIIDFFRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILL
                     YALGIIAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPS
                     SGAFFSCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLL
                     SDVSALELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNM
                     GSERELPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFV
                     VAIRLRHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMV
                     MKRNKTV"
BASE COUNT         9537 a        10563 c         9737 g         8858 t
ORIGIN      
        1 cttccgcgca aaatatattc aacacaaata tagacctgaa gcggtaaatt accaggctga
       61 aaattctttt tatattgtca ggtatttctt aaattatctt aatccttaga caaggaaata
      121 aatcagttcc agatttacaa cgccatcatg gacgaaaaat gaagctttca atctcagcga
      181 cggtgcgcct caccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac
      241 agcgcgcccc gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat
      301 cctctctctg atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc
      361 tgaagttgct caaaaagttc aggcgcgtat tgcagccgga ctgcgggcac caggactggc
      421 cgttgtgctg gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc
      481 ttgtgaagaa gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc
      541 ggagctgctg gagcttatcg atacgctgaa tgccgacaac accatcgatg gcattctggt
      601 tcaactgccg ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga
      661 caaagacgtg gacggtttcc atccttacaa cgtcggtcgt ctgtgccagc gcgcgccgcg
      721 tctgcgtccc tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac
      781 cttcggcctc aacgccgtgg tgattggcgc atcgaatatc gttggccgcc cgatgagcat
      841 ggaactgctg ctggcaggtt gcaccactac agtgactcac cgcttcacta aaaatctgcg
      901 tcatcacgta gaaaatgccg atctattgat cgttgccgtt ggcaagccag gctttattcc
      961 cggtgactgg atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa
     1021 tggcaaagtt gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac
     1081 gcctgttccc ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgctaca
     1141 ggcgtgcgtt gaatatcatg atccacagga tgagtaacat ggcgacattt tctttaggta
     1201 aacatccgca cgttgagctg tgcgacttgc tgaaactgga aggctggagc gaaagcggcg
     1261 cgcaggcgaa aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc
     1321 gcaaacgctg caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg
     1381 ttgttgcctg atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg
     1441 ccctctacgc cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat
     1501 gccaaccgtt attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa
     1561 agtcatccct ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga
     1621 cgtattgtcg tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac
     1681 ccattcgctg gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg
     1741 gttcagggct gggctgattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag
     1801 cttgctggat tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga
     1861 tgaacgcttt ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta
     1921 caccacgccg tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg
     1981 gatggtgctt atgggaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat
     2041 ccggcaacag cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac
     2101 gatccccatc tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt
     2161 acgggcatcc agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc
     2221 ctgcgcgccg ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga
     2281 aagtttacgc aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg
     2341 gtttacttca cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc
     2401 gtccatcgct tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt
     2461 atctgtgccg cgcagcgcag tgtagagacg ctccagcgcc gcacgcgcct gcttcaggtt
     2521 ctcttcgctg tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt
     2581 ttcagcatcg tagtatttca gcacatcgcg cacggtaaaa aagttaccca gcgatttgga
     2641 catcttctcg cggtcaacca tcaccattcc ggagtgcatc cagtagttaa catactgacc
     2701 atcatgggca caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga
     2761 accgccgccg tggatatcaa agtggttacc cagctgcttg cagttcattg ccgaacattc
     2821 aatgtgccag cctggacggc ccgcgcccca cggagacggc cagctcggtt cgccctcttt
     2881 cgacatcttc cacagaacga agtccatcgg gttgcgtttg tcgtcgacca cgtcaacgcg
     2941 cgcgcctgcc tgcagctggt cgagatcctg acgcgacagc acgccataag ttggatcggt
     3001 cgggacgtcg aacatcacgt cgccgttgtc cgccacataa gcgtgacctt tggcgatcag
     3061 ttgttcagtg agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg
     3121 gcgcagaatg ttcaacgcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag
     3181 cgccacaaag ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt
     3241 aatgttgcgc acatacttca gcttatagcc gaggaaacgc aggtaacgcg caaccacgtc
     3301 aaaggcaaca aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca
     3361 cacgtacatg ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag
     3421 agtattgaag atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc
     3481 gtataatacc catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg
     3541 gttatgctat aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg
     3601 aacaggatgc aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt
     3661 ttgacgataa agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt
     3721 ataacaacac cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg
     3781 aaccgggcat gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc
     3841 tgaaaaatac ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg
     3901 cacagttctt catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc
     3961 aaggttgggg ctattgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa
     4021 tcaaaggtgt tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta
     4081 tcattgaaag cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca
     4141 tctctgcgtg gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc
     4201 tcgcaaggcc gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga
     4261 cgatcccaat ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg
     4321 cgttccctgt tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg
     4381 tgaaagtggc atgacgttat tgccggaaga aaaggtgctc gaactttatg gtcgccgggt
     4441 gttgattatg catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc
     4501 caaagtccac aaaccctggc tgcaaatgct attcctcgct ctgccgttgt ttgtgcgcaa
     4561 acgcattgcc gcgcgaatgc gcgcgaacag taaagaagcc aatagcagta aatcgctggc
     4621 gatcatggac gttaaccaaa acgcggtagt cagtgcgatg gaaaaacatc aggtgcaatg
     4681 gctgatccac gggcataccc atcgcccggc ggtgcatgaa cttatcgcca atcagcaacc
     4741 tgcttttcgc gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc
     4801 ggatgacgtt gagctaattc attttccgtt ttaaaaaacc cgcaactttg ctgatttcac
     4861 agccacgcaa ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg
     4921 agagttgtgc accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt
     4981 cgccatcgtg atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt
     5041 cgaaatcctg aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa
     5101 actgttcagc ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc
     5161 aggcggcgca gcgcatctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg
     5221 cgtgccagta cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat
     5281 gccgcgcggc attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc
     5341 gttactggca gcacaaattc ttgcgactca tgataaagaa ctgcaccagc gtctgaatga
     5401 ctggcgcaaa gcccagaccg acgaagtgct ggaaaacccg gacccgcgag gtgcggcatg
     5461 aaacaggttt gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa
     5521 ccgttaggca ttgctgtctg gccagtcggg ctggacgctg aaccggcggc ggtgcctttt
     5581 caacaaagcg tgattaccgc tgagatagaa cgctggccgg aaaccgcatt aacccgcgag
     5641 ctggcgcgcc atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg
     5701 actcagaagc agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc
     5761 gatcgcagcg agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt
     5821 cgcactggtg gctatgacgg tcgcggtcaa tggcgtttac gcgcaaatga aaccgaacag
     5881 ttaccggcag agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa
     5941 gtgtcgctgg ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat
     6001 aacctgcatc aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag
     6061 cagcaggcgc aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatgtgggc
     6121 gtgatggcga tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg
     6181 cgtgtgcata acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg
     6241 catctgcggg cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg
     6301 atgatcaatc tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat
     6361 ctgcactggt acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc
     6421 gacagcgaca catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgccg
     6481 gaatatgcca gcggcgtgat ttgggcgcag agtaagttcg gttaactggt gctctattct
     6541 tgccggatgc ggcgtaaacg ccttatccgg cctaccgatc cggtacccat tgtaggcctg
     6601 ataagatgcg tcaagcatcg catcaggcat tgtgcaccaa ttgccggatg cggcaccggt
     6661 tgtaggcctg ataagacgcg tcaagcgtcg catcaggcac aaatgtctaa tgcctacgac
     6721 tacagcgaaa tacaggtccc cgctttgccc gccagcgtct cttcaatccg cgataacgcc
     6781 ccaatccacg cgggtttacc acggcttctg acatagccac tcaccgccat aaccttcggc
     6841 cccatcgaac catcggcttt ggcaaatggc gctaactcat ccggtgtggc atggcgaatg
     6901 gcacgttgct gcggcgttcc ccagttttca tataccgcat cagcatcagt gaggatcacc
     6961 agcccatcgg cattaatctg ctcagcgagc aacgcagcag cgagatcttt atcaatcact
     7021 gcttcactcc ctgccccatc ttccgtcaca ggcacaccgc cgccgccact gcaaatcacc
     7081 acatgcccct ctttgagcaa caactcgatg gcttcgctgt cgagaatttt acgcggttgc
     7141 ggagacgcca ccacccggcg taaatattta ccatcacgtt tcatctgcca gccgtaagcc
     7201 gcttccagtg cctcttgttc ttctggctga taaaccggac caataaattt ctctggctgc
     7261 aaaaacgccg gatcatcagg cgaaacctca atgcgcgtca gcaccgtcgt aacaggcggc
     7321 atctgcggct gtgcgctcaa gctctgcgcc agcatatagc caatcatccc ctggctttcc
     7381 gccaccagca catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc
     7441 agcagcccca cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagt
     7501 cgtgccagcg cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc
     7561 tcaccgcgct ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc
     7621 cttgtaataa ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc
     7681 ccagcgccag cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga
     7741 caaagtgcag gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc
     7801 tcaccgctgt ggtaacgagt tgaagattgt cggaataggc aaaaaactgg cgaccagagc
     7861 gcgcatctaa agcgccataa taccaggccg ccagcagcat tccgctcagc gtgtcatcat
     7921 gactcggcgt taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat
     7981 tgagcgcggc ctgaaaacag tgacgaaact ggcgtaactc agcaggcagc ggatcgcctg
     8041 ccgccaacgc cagtggcccg aaaagcccgg tttcctccgc gcgttgcatc catgcagctg
     8101 caagcggttg aggatgcgca ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt
     8161 taaccgtgaa acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc
     8221 ataatccacc gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga
     8281 aaccactacc ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg
     8341 cccgtcgcca cactcccgct aaccgccagg actggcgata attcggtgca ctacggctgg
     8401 caagcagagg atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc
     8461 agtgcttcca gcgcctgttc aaaacacgcc agcggtgccc gcacggtgcc tgcgccaatc
     8521 tgcccgatcc ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt
     8581 tctaccacgc gacgaatgtc cagccccagg cacgcgccct gaaaatccca gcctgggatc
     8641 tgcaactgca tattgcgctc aaggtaaatt tccgccatct cttcagatac cgctctggcc
     8701 gcttccatgc cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca
     8761 cctccgatac caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc
     8821 tgcgagaagc cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt
     8881 tcgcccagcc cgcttacccg aatcccgaac atattgccgt tgcgggtcat tgccgtgacg
     8941 atgctgcctg cgcggatcat cgcgccagca tccatcgccg ccttgcagta agccatcgcg
     9001 aggttgagga agaactgatc ggtcacgctg aggaaatcca tcacttcggc gatgtgctgt
     9061 ttatcatgat cgaggcgagc aatttgtggg gccagcgcac gcattaacag tgcagaggaa
     9121 gcaatattgc gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg
     9181 agatcgatac cgcgctccat gcgccccagc gccgcgctta atactggcat taacacatcg
     9241 cgcatccagc gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg
     9301 ccgatacctt cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc
     9361 agcatcggca tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga
     9421 caaggaatga agttcacttc cccctgctcc agcatcgcca gtgcctgcgc ttcatctttc
     9481 gcccagcctt cgaacagaca tgcgcccacg cacgcccctt tcatgggtcc ggtcatctcc
     9541 tgccagcgca ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata
     9601 agtgaaaaag ccggttgcac atccagccag tgtggacgag cacgacgaat ttgttcgata
     9661 accgcagcat tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct
     9721 aataaacgag ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc
     9781 ggtttgctgg cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg
     9841 acgttttcca gtaatgacgg tgtatgttgc tgtgcggcag gcgagagcgg ataaattaac
     9901 gctgccgcca gcaaggtggc ttccggcagc gaactcacta ccgcgatccc cgcatcttcc
     9961 agcttggcga tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt caccgtggca
    10021 atggcataca gtggttgatt atctgaacgc gcggcacagg ctttttgcca ggcgcttacc
    10081 agcgaggcgg caggatcggc agtcgcaccg aagccaatca cgacatcaag tagcaatacg
    10141 cgcacttgtg gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgggtcgatc
    10201 atcggatggg gacgcccgac ggtgtagaaa tcatcgccga ggtcaataat ctggtgacca
    10261 tcggcgtcga gcatcatgcc gtgatgatgg gtatcgtcgg cttccacgcc aaggtgtcca
    10321 gcaagtaatc ccgccgcttc ggcagccagc gtaccgccgg tatacaaacc gcaaataaat
    10381 ccgctgctga caggcgctat tgcgttacgt cgcgccgtga cgcgtgaaag caggcaagcc
    10441 aggcgtgcgg cctcatccag cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc
    10501 ggggtataac ctaaaaacag cgccaccgtc ggtttgccgg ttgctttcat ggcattaaca
    10561 attttcagac gcacagcttc ggcaggtggc tttgaaacaa atgccagcac ttcgcttttc
    10621 tcgtctgcac tgagcatttc cagcgccgtc agcgcactga tgccgcctac ttcacggctg
    10681 aggtcgcgcc cgccaagacc aatcgcgtga gtaattccct cccctgcctg cgcaatctgc
    10741 gaacacagtt cctgaatccc ggtaccggaa gcgccaatga cgccaatgtt gccttccggc
    10801 atcacgttag caaaagccag cggcgtgccg gcaatcatcg acgtaccgca gtccggcccc
    10861 atcaccagca agcctttttc tcgcgcgcgg gttttaagtt ggatttcatc ttccagcgtg
    10921 acgttatcgg agaacatcat cacgttgagg ttgcgatcca gcgcctgatt cgccagctcc
    10981 gccgcatact cgccagccac tgaaatcagc gccagactgg catcgggtaa tttctgacag
    11041 gcgctgtccc agcgacgcac ctgcgtcaac gcctggctgc tgcctgaccc ctgcgccagt
    11101 tgttttagcg cctcttcaag ctgctgcata atcgcctgcg cgatccccgc atccgccgct
    11161 tcgctacgaa ttgccacgca aatatcgttc ggcgtggcgt tattaaaatc gtcatgccag
    11221 aaacctgtgg tatctaataa cgctttattg gcgggcgtac ccatcattac ggaaacatca
    11281 tcaacatttt ctgattcgct gagttttcgt gaaataatca ttaaactgac cgaatcctga
    11341 aaacaccctt ttttaataaa ggcgtggatc ataacaactc cttaaaaatt gcacccatgg
    11401 caattgcacg agcagtgtta atgtggcgat tacggtatcg atgacaacgg gtaaataagg
    11461 tgatgaatat cacacaggga ttattaacaa aagaattcaa ataaccgcca tgctattacg
    11521 tgttattttt ttaataagcg tggttgtttt aaaaaccagg ttaaaagcga gcggattatc
    11581 aaataaattc taactatatt tttttgcctg tctggatcac ataatccaga tattttccct
    11641 gtatgttaat tgcagtcatg cttcacaccg tcgttaaaaa ggaagacaga tgaaaatcag
    11701 tcgggaaaca ctccaccagc taattgagaa taaactctgc caggctgggt taaaacgtga
    11761 gcacgctgca accgtggctg aagtattggt ttacgccgat gccagaggga tccactctca
    11821 tggcgcggtg cgcgtggaat actacgcgga acgcatttca aaaggcggca ccaaccgcga
    11881 accggagttt cgtcttgagg aaaccgggcc gtgctcggca attttacatg ccgacaatgc
    11941 cgccggacag gtcgcggcga aaatgggtat ggaacatgcc atcaaaaccg cccagcaaaa
    12001 tggcgttgcg gtggtcggta tcagccggat gggtcacagc ggcgcaatct cttattttgt
    12061 gcagcaggca gcccgcgccg gattcattgg catttcgatg tgccagtccg atccaatggt
    12121 ggtgccgttt ggcggcgcgg aaatttacta cggtactaac cccctggcct ttgccgcgcc
    12181 gggagaaggc gacgagatcc ttacctttga tatggcgact accgtacagg catggggaaa
    12241 agtgctcgac gcccgctcgc gtaatatgtc tatcccggat acctgggcgg tcgataaaaa
    12301 cggtgtacca acaaccgatc cgttcgcggt acatgctctg ctccccgccg ccgggccaaa
    12361 agggtatggc ctgatgatga tgattgacgt cctctcaggc gtcttactcg gcttaccgtt
    12421 cgggcgacag gttagttcga tgtatgacga tttacacgca gggcgtaatt tggggcaatt
    12481 acatatagat attaatccga actttttctc ctccagcgaa ttattccgtc aacatcttag
    12541 ccagaccatg cgcgaattaa atgccattac ccccgcgccc ggttttaacc aagtttatta
    12601 tcccggacag gatcaggata ttaaacaacg caaagccgcc gtcgaaggca tcgaaattgt
    12661 tgatgatatt taccagtatt taatttccga cgcgctttat aacacgtcat acgaaacgaa
    12721 aaatcccttt gcgcaataat tattgagaca ggactttctt atgattacac attttcgtca
    12781 agccatagaa gaagcgctgc cctggctttc ctcttttggc gctgacccaa cgggtgggat
    12841 gacccgttta ctttattcgc cggaatggct ggaaacccag cagcaattta aaaaaagaat
    12901 ggcagcaagt gggctggaaa cacgtttcga tgaagtaggg aatttatacg gtcgcctgaa
    12961 tggtaccgaa tatccacagg aagtgattct gagcggttcg catatcgata ccgtggttaa
    13021 cggcggtaac ctcgacgggc aattcggcgc gctggcggcg tggctggcaa ttgactggct
    13081 gaaaacgcaa tacggcgcac cgttacgtac ggtcgaagtg gtggcgatgg cagaagaaga
    13141 aggcagccgc ttcccgtatg tcttctgggg cagtaaaaat atctttgggc tggcgaatcc
    13201 tgacgacgtg cggaatatct atgatgccaa aggaaatagt tttgtcgatg cgatgaaggc
    13261 ttgcggattt actctgccga acgccccact aactccgcgt caggatatta aagcctttgt
    13321 tgaactgcat attgaacagg gctgtgtgct ggaaagtaat ggtcaatcaa ttggcgtggt
    13381 gaatgcaatt gtcgggcagc gtcgctatac ggtgacgctg aacggcgaat caaaccatgc
    13441 aggcaccacg ccgatgggtt atcgtcgtga tacggtttac gctttcagtc gcatttgcca
    13501 tcagtcgatc gaaaaagcga aaaatatggg cgatccgctg gttctgacct ttggcaaagt
    13561 agagccgcgc ccgaatacgg tgaatgtggt gccgggtaaa accacgttca ccattgattg
    13621 tcgtcatacc gacgccgccg tgctgcgtga tttcacccaa cagttagaaa acgacatgcg
    13681 ggcgatttgc gatgaaatgg acattggtat tgatatcgat ttatggatgg acgaagaacc
    13741 cgtgccgatg aataaggacc tggtcgccac cctgacagaa ttgtgtgaaa gtgaaaaact
    13801 gaattaccgg gtgatgcaca gtggtgccgg gcacgacgcg caaattttcg cgcctcgcgt
    13861 gccgacctgc atgattttca ttcccagcat caacgggatc agccataacc cggcggaacg
    13921 caccaatatt accgaccttg ccgaaggggt caaaacgttg gcactcatgc tttatcaact
    13981 tgcctggcag aaataaggag tcataaatgg gatatttaaa taacgtcacc ggttaccgcg
    14041 atgatttact ggctaaccgt gcgattgtga aacacggtaa tttcgcactg ttaacaccag
    14101 acggtctggt aaaaaatatt attccgggct ttgaaaattg tgacgcgaca atcctctcca
    14161 cgccaaagct gggtgcctct tttgttgatt atctggtcac actgcatcaa aacggcggca
    14221 accaacaggg cttcggtggc gaaggcattg aaacgttcct gtatgtgatc actggaaata
    14281 tcactgccaa agccgaaggc aaaacatttg ccttaagcga aggtggctat ctttattgcc
    14341 cgccaggctc cttaatgacg tttgttaacg cccaggccga agacagccaa atctttttat
    14401 ataagcgccg ctatgttccg gtagaaggcc atgcaccgtg gctggtttct agcaatgcca
    14461 gcgaactgga acgcattcac tatgaaggta tggatgatgt gatcctgctg gattttctgc
    14521 ccaaagagtt aggttttgat atgaacatgc atatcctctc ttttgcacca ggtgccagcc
    14581 acggttatat cgaaacacat gttcaggaac acggtgccta tattctttcc ggtcaggggg
    14641 tttataacct cgacaataac tggatcccgg tgaaaaaagg cgattacatc tttatgggcg
    14701 cttattcttt acaggctggt tatggtgtag ggcgtggtga agcgttcagc tatatttact
    14761 cgaaagattg caaccgcgac gtagagattt aagtcatacc actcgcctga ttatttttag
    14821 ctatgttgga ttttgccacg gtttataccg tggctttttt tgtagtaaat tcaggtaaaa
    14881 aagatgcgaa ataaatcgca tccgctttaa aggtaagagt cagtttttaa ttccctgacc
    14941 tattttaatg gcgcaggcaa tatttcgcgc gctgttgaag agattggttt caccgcttgc
    15001 gagcacttcg gctaaaggtg ccagacgagg caaaatgctg aataccgcgt caatgccgta
    15061 ctggtgcacc acttccacgc catcacccaa tacgccagca atcccaatca ccggtacatt
    15121 aaactgcttc gccaccgacg caacgcccag cggcgcttta ccgcctgccg tttgcgagtc
    15181 gatgcgccct tccccggtga tcaccagtgc tgcgccctgc actgcctgcg caagattgac
    15241 tgcattcaac acaatctcaa tgcccggttt aatatccgca ttgaggaata ccgccgccgc
    15301 aatccccata ccgcccgcag cgccgccgcc cgccatctgg cagacgttga tttcagtttg
    15361 ctgttgtaaa acacgggcgt aattttgcag cccctgttcc agctcttcga ccatttccgg
    15421 cgttgcccct ttttgcgggc caaacaccgc cgccgcgccg cgtgccccta ccagcgggtt
    15481 atcaacgtca catgctactt caatatggca attcgccaga cgcggatcgc attcatccat
    15541 ctcaatgctt gccacgcgcg ctaaattacc gccgttagcc accagcacct gaccgtcggc
    15601 atcaaggaaa cgcacaccga gcgcctgcgc catgcccata ccgccgtcaa ccgtcgcact
    15661 gccgccaatg ccgagaataa tatggcgaat gccgttatcc agcgcatgac gaattaactc
    15721 ccccgtacca aaactggagg ccagcaacgg attacgcttt tcaggcgcga ccagcatcag
    15781 gccacttgct gccgccatct caatcaccgc cgttttcccg tcgccggtaa ggccataaaa
    15841 agcattcact ttttcgccca tcggcccgca gacttcaagc gtcacgatgt tgccgcccgt
    15901 cgcggcgacc atcgcatcca ccgtcccttc gccgccatcc gctatcggca aacagatata
    15961 gttcgcatcg ggaaagaggg tcgaaaaccc ggctttaatt gcctgacaac atttttctgc
    16021 acttaagctc tctttaaaag agtctggcgc aatgacaatc ttcattatca atcctttttc
    16081 aacaaattat tcgatgcgtt cccacggaat aaggttatcc ataagaacag ccagtaaaat
    16141 cccgaccaat aagccgttac tgagcagagg acgaagcgtt aatggaaggt cttgcagata
    16201 cacaggcggt aatgccatta aaaatatgcc gacaaataac ggcaatgcga gtcgataaat
    16261 attgcgagcg gtaaacgtta tttgctggct aaacaccagc gcggaaaaga gtaaaggcag
    16321 ataagaaacc agcatgaccg cactactcac gggtaaaggg atactgcaaa acagttgcgt
    16381 gagtgccgga accagcgcca ccagcaggca aataacgctg ccataaataa acgaacgccg
    16441 cgtgtaatcg ccagtctggg ttaataaacc aatggatgaa acaaacggtg aaaatggaat
    16501 tactgccagc ggcacggtta tcagtgtcat aaatccggtc gccacaaagc tacgacgata
    16561 acgcgtattc cccgcgccct gctgcggata aaaaacatcc gtgccccgaa tcgcaccgta
    16621 ggtattgctg atattcacca gacctgtaat caccgccgtc agaataatcc ccggcgacaa
    16681 agcaccgtca ctgccgagcg ggaaccactg ccagtgcaac tcaccggaga gcgaatgcga
    16741 agaaggaaag caaaagaacc acaacaacca gccggttatc gtaccgacca gcagaccata
    16801 acgggcaaaa cgttgcggca ggaaaataat catcgccagt accaggcaca tcaccgccac
    16861 cgagagcgcg aacggcggta actgaatttt aaaattcggg tcggctatgc caaacggcag
    16921 cccgagcata cctttgaaaa agatagtggt cagctgcgcg cccagcatca acataaacaa
    16981 gaccatcacc gacggcgtaa ataaccgtgc caggcgatgg cctaatccgc taaaaccaat
    17041 caacatcgtc aacacgccgg agagtgcaat ccccaccgcc agactggtag cgatatcgtt
    17101 gatcggtgtc ccgcgcgatg cttcgccaag ggtgatagtt aggatggttc cccaccacag
    17161 gccccccggc ccttccataa tagcgcgacg atgaccgcaa aacgcctgag cgaagcaggc
    17221 cagtgcggta gcaagaaaag catattgcgt gagcgtaagc aggctacttt gcggcaactg
    17281 aaaagcagaa agtagcgtag gaggaaccac aaccgtgttg caaaaaataa agaaaaacca
    17341 ctgaaatcct gataacaggc tttcgcggct aactgcaaaa ttgaacataa acacacctta
    17401 accggagaat gcccgccgca aggacgggca ttgcaggggc cagattactg ctgatgttta
    17461 aggataaatt gacctttcgg cgcaacaggg aagccctgtt caatgtcgta aatcacatca
    17521 ccacgtaaga tggttttcgt gatacgcgcg ccaatggtac ggccaacata cgggctgact
    17581 ttgtggcgat attccagatc gtcattggta agaacatagc tgctattcgg ctgaatgaag
    17641 acgaagtcgg catcttttcc tggggcgata cggccttttt gctgcagacc gaaaatatct
    17701 gctgcgttag tcgccattaa tttgccgaac attggcagag acattccgcg tttctgtacc
    17761 gcttcatcga acatcacgtc catacagttt tgcagaccgg caataccgcc ccatgcttcc
    17821 atgatgttgc cggctttcat ttccggaggg catggtgagt ggtcggaaac caggcagtcg
    17881 atttcaccgt taaacagttt ttcccacatg cctttctggt tttccagatc gcggatcggc
    17941 ggtgaacact tcgccagagt accgatttct tcgaactgat cggtatccag tacaaagtaa
    18001 tgcgggcagg attcacaagt aacgtcctga ccttcctgac gtgcacgagt cacttcttca
    18061 acgccttccg ggctgctgat atggcaaacg tgcagacggc aaccggcaac tttcgccaga
    18121 tacagcacgc ggcgaatcgc ttccacttcg gtaaataccg gacgcgaagc cacatagtca
    18181 tgggcagtta cgcgaccttc acgtttcgct tcttcgccca gtgcgtcaca gatcagcgcg
    18241 ttttcgcagt gcaccagcac cggctgcccc agttcgccaa gcttctgcgc acctttgaag
    18301 aactgccagt cgtttacatc acggaagtcg ttgtcgatac cgcgatcgcc acaggtcgca
    18361 acgaagcatt tgaagccgac aacgcccact tcatccagct catgcagacg gtcgatgttg
    18421 taagacacca ggccaccgag ttgtgccgca tcgatagtta gcttgccttt agcggcatcg
    18481 aacttcagtt caattgaagc gcggtcaacc gttgcaggca gctggttgag cggcatttcg
    18541 atcatggtgg tgataccacc ttttgccgct gcgcgagtac cggtttcata accttcccag
    18601 tggctacgac ccggttcaga aatatgggtg tgcgcatcaa ccatgcccgg cgaaaccacc
    18661 agaccagacg catccataac ttcttttgca tcgcccagat cctgaccgat agcagcaatt
    18721 ttcccgcctt taacggcgat atctacaacg cgagcttcgt tttctaaaat aacggtgccg
    18781 tttttaatga ttaaatcaaa agacataaca aactccttgt aaaatacaga ttggttttta
    18841 actcaatgtt caatatcggg attaattaac ctatggtttt ttgctctcct gttttttctg
    18901 ctgttgtacg tttctttaat aaggcgtagg ccgcaaaggc gacgatgacg ccgacaaacc
    18961 atgaaacacg cgataacggt tccataaagg gaataaactt accgccaaga gataaaataa
    19021 cggccaccag agttactgaa aacgcggtga ggttaaaacc gttatcgtaa tatttataat
    19081 cgccaggtgc ggtgtacagt tcatcaagat taatttgccc acgcatcacc acaaaataat
    19141 gtgccatcat gacaccaatt accggaccca gcattccgcc gatgatatcg aggaacagat
    19201 aaatactgtc ctgattttcc attaatttcc acgggcagat cagcaggctg ataatactgg
    19261 caatcagtac gccgttttta taggtcagtt tcgtcggtgc aatagcggca atctgataac
    19321 cggccggaat aatattaccg gtggcgttag tggaaatggt cgtcatcaga ataaccagta
    19381 ccgcaaagaa cgaggcgaac aagctgtccc aacgctgaac aatatccagc acgttccagg
    19441 tgtcagcgcc gtagtgaata ctggctccgg caataataca taccccggcg accgcaaaca
    19501 gaatatagac cacaactaaa cccagcgttt gccccagcgc ttgctcacga aacgagtggg
    19561 cgttttgcgt aaagtcggat gcgctcaccg ccggtgccgc ccagaccgca actaccgcgt
    19621 taatcaccac caggaacaag aatccactgt tttctgcttt ctgaataccg ctcggaatgt
    19681 agtcaaagat tggaccgagc ccgaccagcg aaatcgccca aatcgccata ccgccgaaaa
    19741 cgatatagat gcacgggtta agaatggcag tgaatttatt taaaactttg ccaccgccaa
    19801 aacctatacc aacgttgacc agccagaaga gtaagaaagt aattaagccc ggtagagaaa
    19861 ggcctaacag agtgaaatca ccaccgagag tcaaaaatcc cggccagatt ttgccaatca
    19921 gaatcaagca ggccagtgac cccgcgtaac attgcaggcc aaaccacata atggcagcaa
    19981 taccgcctct taataatccg ggaaacagtg cgccacgtac gccgtaagaa gcacgcagga
    20041 tcatggcaaa cggtacgccg tatttactgc ccgcagcacc gtttaatacc attaccgcgg
    20101 caatgaaaaa ggcgctgagg ataattgcca acataatact aaaggtagac aagccgagaa
    20161 taaaaaagcc gccgaccatc acataattgg gaacgttatg aaccgaaccc atccataagg
    20221 taaaatagtt aaatgttttc caggtccgct ggctttgcgt tttcggcaat agatcttcgc
    20281 tatagccgcg ttgctggaat agttttctct gatgttccat aaatccctct taatttcacg
    20341 tatcgaataa cgctgtttta atctagctat gaggaacaaa taaaataaaa atattatttt
    20401 taatactgaa gcaacatctt tggctgcttt cttttttaat attgctggcc gattcatatt
    20461 ctgatatcgg ccatttttat tgcgggtatc aggccagttt atgattcgcc attaattcca
    20521 gcgcctgcac taacgcagag tgatccaact ggctgccacc gtttgccgca caggtattaa
    20581 ataactcctg gcaggtcgcg gtgtttggca gattcagcgc cagtgtcttc gcactttgca
    20641 gtgccaggtt gagatctttc tggtgcagcg caattttgaa acctggatta aaggtgcgtt
    20701 taatcatgcg ctcaccgtga acttccaaaa tacgtgagga agcaaagccg cccatcagcg
    20761 cctggcgcac acgtaccggg tctgcaccgg cttttgaagc aaacagcaag gcttcagaaa
    20821 ccgcttcaat attgagcgcc acgataatct gatttgccac tttgcaggtt tgaccgtcgc
    20881 cgttaccgcc cacgagggtg atatttttac cgagcaattc aaacagcggt ttaacacgtt
    20941 caaataccgc ttcgtcaccg ccaaccataa tcgacaacgt cccttcacgc gcgccgattt
    21001 caccgccgga gactggcgca tcgagataat caccgcccag ttcattcacc tgacgagcga
    21061 aacgtttagt ttcaatcggg gaaatggagc tcatatcaac aatggttttg cccttcagcg
    21121 aggctttggt acaaccattt tcaccgaaca gaacttcttc aacctgtggt gtatccggca
    21181 ccataataaa aatgatgtcc gatgcttccg ttacctggcg cgcagtttca acactgacgg
    21241 caccgagtga cagtaattca tcagcaaccg gaccaatggt tgtgacatgc agttgatgac
    21301 cggcacgcgc cagattaatg gccatcggtg tacccataat gcctaagcca ataaatccca
    21361 gtttcataat taacctcttt taaatttcgc ttttcctgaa ttcagacaac acgatcccct
    21421 gccaacttag cggcctaaaa aagcattgcc tgaacagcgt tacgttttaa cggtacggat
    21481 ccatccagcg taaaccggct tccgtggtgg tttggggttt atattcacac ccaacccaac
    21541 cgttgtagtc agaattttcg attaccttaa agagataatc ataattaatt tctccggttc
    21601 ccggttcgcc gcgatgcgga ttatcagcaa tttgcaggtg accaatttta tcggcccact
    21661 gagtcatggt gttggttaat tcaccttcca tccgctgcat gtgataaatg tcatactgaa
    21721 ttttcaaatt gcagcaacca acatcatcaa tcaatttcag cgcctgccga gttccggtga
    21781 gatggaaacc aggaatatca aaatggttaa taggttcaat cagtaataaa atatcttctt
    21841 tcatcagcat attcgcggca taacgcaggt tttctacaag cgttgcgtga atctgttcac
    21901 tgctgaaacc agccggcgtt ttaccgacca gacagttaat ttttttattc cccagcgcac
    21961 gggcataacg aatcgctgct gctacgccat cccgaaactc ttcttcacgg ccaggaatac
    22021 aggcaatacc gcgctccccc gccgcccagt cacccgccgg taaattgtgc aacgtatgtt
    22081 cgagtttatt actcgccagc acctgtttta attcttcaat gtcgtagtca taaggaaaca
    22141 taaattcaac gccgcgaaaa ccacactgcg cagctttctc aaaacgggcg agaaaatcat
    22201 attcaccaaa taacatcgat aaattagcag agaaacgtaa cattatttat ctcccttatt
    22261 catagtgcat gaagcaggtt tcagtcggtg catccgctgc gttatcggcg atatcttcaa
    22321 attccatgac gttatccagt tcgctgccca tcgaaatatt ggtcacacgc tcgagaataa
    22381 cttccacgac taccggtacc cgatattgcg ccattaaggc tttcgcctgt tcaaaggctg
    22441 gcgcaatatc ttccggtttg aagacgcgaa tcgctttaca acctaaacct tccgctactt
    22501 ttacgtggtc gacgccgtaa ccgttcactt cgctggagtt gatattctcg aaagcgagct
    22561 gtacgcagta atccatgtca aaggcacgtt gtgactgacg aatcaggccg agataggcgt
    22621 tattcaccag tacatggatg tacggaatgt tgaactgtgc gccaacggcc aattcttcaa
    22681 tcaggaactg gaagtcgaag tcaccggaaa tcgccaccac attccgttct ggatcagcag
    22741 cacacacgcc cagcgccgcc ggaatggtcc agcccagcgg gcctgcctga ccacagttga
    22801 tccagtgacg gtctttaaag acatgcagca tttgcgccgc agcgatttgt gacaggccaa
    22861 tggtggtgac ataacaaaca tcgcgaccaa aggctttgtt catctcttca tacacgcgct
    22921 gcggtttcac cggcacgttg tcgaagtggg ttttgcgcag caaagtgcgt ttgcgctgct
    22981 ggcagtcggc gacccattct ttacgacacg gcaggcgacc cgctttttgc atctcctgcg
    23041 ccacttcaac cagcagtgtc agcgccgctt tagcatcaga gacaatgccg agatccggac
    23101 acagcacgcg accaatttgc gtcggctcaa tatcgatatg aacgattttg cgcccttcgg
    23161 tgtatttctc taccgaaccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca
    23221 tgtcggacgc cagcagcgtt gcgttaccgt aacgatgcgc ggtttgcaga cccaccatcc
    23281 cggccatcag ttcatgatcg tccgggatac agccccagcc catcagcgtt gggatcaccg
    23341 gaacgctggt cagttcagca aactgttgta acagtgcagc tgcgtcagca ttaattaccc
    23401 cgcccccggc aacaatcacc ggacgttcgg cctggattaa catttctaca gctttttcga
    23461 tctgcatacg gctggcagca ggtttgtaga ccggcagcgg ttcgtacatg tcaggatcaa
    23521 actcgatttc cgcaacctga acgtcgaacg gtaaatccac cagtaccgga cccggacgac
    23581 cagaacgcat caggtgaaat gcctgttgca gcacgcgagg caccagcgcc gcttcacgta
    23641 ctgtaaccgc cattttgctg accggtttag caattgcttc aatatctacg gcctgaaaat
    23701 cttctttatg cagacgggcg cgcggagcct ggccggtaat gcacagaata ggaatggaat
    23761 cagcagaagc agaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca
    23821 gacatacgcc gatattccct gccattgcgc gggtataacc ttccgccatg tgcgaagcac
    23881 cttccacatg acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga
    23941 acggattgat tgcagctccc ggaacgccga aggcggtggt gataccttct ttctccagca
    24001 catacattgc cgcgtcaacg gctctcattt ttgccatttt cttcctacct ctattaattg
    24061 gaaaaatttt ccaactttca aattttgtca aaaaaacccg ctccgcagag cgggatgcga
    24121 cagacactat ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct
    24181 gaccagctca ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat
    24241 ggagatagcg gcaacaacac tacctacatc atcgtaaatt gctgaagcta tgcaattcag
    24301 acctacaaca tgctcttctt tatctacggt atagcccagt tcacgcgctt gctccaggtc
    24361 cttcagtaag gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg
    24421 cagaatgatg ctcatcaact cctcttccgc cagcggataa agcagcgctt tgcccgcacc
    24481 ggaagcatgc agtggcagac gactgcccag tggcgcacac atcctgacca tcgatttaca
    24541 ctctaactga ccaattaata ccgcttcatt gccgttacgg atcgcgacat tgaccgtttc
    24601 gccggaaagt aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt
    24661 atggatgtac gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaactg
    24721 actgtcctga tagacaaaat ccgctgcctg taaaaccttc agcaagcgaa aggtcgtgga
    24781 gagcggcaaa tccagattga gagaaatatc actaaccgac gaacttcccc cacttttttc
    24841 cagatattgc agaatcgcaa ttccccgctc taatgcctgt gcgcccttct gtgccacagg
    24901 ctccgcctgt cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac
    24961 ctacaagtga gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt
    25021 cacgcaaaac agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg
    25081 cgatcgatgg tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg
    25141 ttacgatggt aattcactcc ctgttcgccg ttggtgataa acgcccgcag cgttgacagg
    25201 tctggtttgt cgtcacctaa cgccacgacc accacaaaca cctcaccttt catcgggata
    25261 aaggcctgag tacccagcgg atgacgttcg agttcgtgaa tggtcagcgg cagattcgcc
    25321 ggttgcgcgc ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc
    25381 aaatcgtggt aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgcgtt
    25441 tcgattacgt cgccataagc actaaaggct tcctgactta acggtaatac ctgaagtttc
    25501 atctttttgt ccttgtaaca taattccgtt caaccaccgc cgaccggtca gcttggtttc
    25561 aataacaggc aatctaaaac cttaagagaa caacatcaat acaaaagttg gaatattttt
    25621 ccaaaaatag atttagtcca caaattgaac agggttatca tgtaaaaata ctgataaatc
    25681 agaggataag aaaatcacgc aacttgcgga tttaaaaggg gatacctgaa gatgttcgat
    25741 ccagaaacct tgcggacttt cattgcggtt gctgaaacag gaagtttttc aaaagcggca
    25801 gaacgattat gtaaaaccac ggcgacgatc agttatcgca ttaaacttct ggaagagaat
    25861 accggagtag cgctgttttt ccgcacgact cgcagcgtga cgttgacagc ggctggcgag
    25921 catctacttt cccaggccag agactggctg agctggctgg aaagtatgcc aagcgagctg
    25981 caacaggtga atgatggcgt ggaacgccag gtgaatattg tcatcaacaa cctgctctac
    26041 aacccccagg ccgtcgccca gttgctggcg tggctgaatg aacgttaccc ctttacccag
    26101 tttcacatct cccgacaaat ctatatgggc gtctgggatt cgctattata cgaaggtttt
    26161 tcactggcta tcggcgtcac gggaaccgag gcgctggcaa atacctttag tcttgatccc
    26221 ttaggatcgg tgcaatggcg ctttgtcatg gcggcggatc atccgctggc gaacgttgaa
    26281 gagccgctaa cagaagcgca gttgcggcgc tttccggcgg tcaatattga agacagcgcc
    26341 cgcaccttaa ccaaacgcgt cgcctggcga ttgccggggc aaaaagagat tattgttccc
    26401 gatatggaaa cgaaaatcgc cgcccatctg gcgggtgttg gcattggttt tttgccaaaa
    26461 tcgctttgcc agtcaatgat cgataatcaa caactggtta gccgggtaat cccaacgatg
    26521 cgccctcctt cgccattaag tctggcatgg cgcaaatttg gcagcggcaa agcggtagaa
    26581 gatattgtga ccttgtttac ccagcgcagg ccggaaatca gcggattttt agaaattttc
    26641 ggcaacccac gcagttaaaa atcaagtttc cttgcgcgtt ggcgcgtaaa atagcgcgca
    26701 agctgatttg aggttcactt ttaactatgc aagagagaca cacggaacag aactatcgtg
    26761 ccctgctgat tgctgatacg cccattattg atgttcgcgc ccctatcgag tttgagcaag
    26821 gcgcaatgcc cgccgctatc aatctgccgt taatgaataa cgatgaacgc gccgccgttg
    26881 gcacctgcta taaacagcaa ggctcagacg cagcgctggc gctggggcat aaactggtgg
    26941 cgggtgaaat tcgtcagcag cgcatggacg cctggcgggc agcgtgcctg caaaatccgc
    27001 aaggtattct ctgctgcgcc cgtggcggtc aacgttcaca tattgtgcaa cgctggctgc
    27061 atgaagcggg gattgattat ccgctggtgg aaggcggtta taaggcactg cgccagaccg
    27121 cgattcaggc gatcattgaa ctgtcacaaa aaccgatagt gctgattggc ggttgtaccg
    27181 gcagcggtaa aacgctatta gtgcaacaac agccaaacgg tgttgatctg gaagggctgg
    27241 cgcgtcatcg cggttcggcg tttggtcgca cgttacaacc acaacttagc caggcgagtt
    27301 ttgaaaacct gctggctgcc gaaatgctaa aaaccgacgc ccgtcaggat ttgcgcctgt
    27361 gggtgctgga agatgaaagc cggatgatcg gttcgaatca cctgccggaa tgtctgcgcg
    27421 agcgaatgac tcaggcggcg attgcggtgg tagaagatcc gtttgagatc cgtcttgaac
    27481 gcctgaacga agagtatttc ttgcgtatgc atcatgattt tacccacgct tatggcgacg
    27541 aacagggctg gcaggaatat tgcgaatacc tgcatcacgg actttcggcg attaagcgtc
    27601 ggctggggtt acagcgctat aacgaactcg ctgcccagct ggatacggcg ctgacaacgc
    27661 aactcaccac cggcagcacc gacggtcatc tggcctggct ggtgccgtta cttaaagaat
    27721 attacgaccc aatgtatcgc tatcagcttg agaaaaaagc ggaaaaagtt gtctttcgcg
    27781 gtgagtgggc ggaagtggcg gaatgggtta aggcgcagtg atgggcggta ttctgaaacg
    27841 gcgattgtcg tgagaacaga atggttgcac cagaatacag atataaaaaa accgacatga
    27901 cagtcggttt ttttattttt tccgcccaag acttggtggt tccgggcaga aatgaaacag
    27961 tcctcatcta gcaagtgaat tataagtcag gatgcctaca tattcaaaca gctatgttgg
    28021 cgatacaatc ccgacgctag tagtgatact gcctcaaggg attaccgcag tgcggaaaag
    28081 tacttttaag tcctggtcag gatacaggtg gtgctgattc tggtgctcta atttggagag
    28141 aggcagtcct catctagcac atgaagaaaa tcatagttac aggcaacgta agggtaagct
    28201 gtgattgtct ttctccctta agggagcaac ggatgaaaac ttttctcgat aaagttctca
    28261 tcgttcttaa ggcgctaatc gcgctgctgg aactgatccg tcagtttatt gaataactga
    28321 ggaaacgtag ctaaggccga aggtttacca ccttcggtct ttaaaaaaac cgacatgaca
    28381 gttggtttca tatttattgc caaaatgcac actgttcatg ccggatgcgg cgtaaacgcc
    28441 ttatccggcc tacacaatct tgcaaaatca atatattgca cgctcttcgt aggcctgata
    28501 agcgtagcgc atcaggcaat ctaacacctg tcatcacccc gcaaactgcc tgaacagcgc
    28561 cttaccctta accagtctcg cgcccagcca gccgccgaaa agcgacagca gcagcgcacc
    28621 gctgcacggc agaacaatcc acaatcgcca gtctggctcc cacgggaaat caaacacttt
    28681 cgcctgcaac actgccagtg ccgtttccgc accaattgcg gccaccaggc cggaaacaaa
    28741 cccaagcatg gcgaactcac accacaatgt cgtgcgcagc agtttttttc ccgcaccgag
    28801 tgtgcgccat accaccagct cctgatgacg ctggcgcatt cccacctgca cctgtgccag
    28861 caacagcaac ataccgcagg cggtgaccag caccaccatc acctccagcg cccgacttac
    28921 ctgctccagc acctgaccga cctgttttaa aatcgcgcca atatctaaca ggctaatggt
    28981 cgggaactgg cggttgagtt gcgtcaacat gccgttgcca ttctcccagc ggaaactggt
    29041 aagccagctc tgcggttgcc cgtctaatgc cccttccggg aaaataaaat agaaattagg
    29101 ccgcagactt tcccagtcca ctttgcgcag gctggtcact ttagcgcgga actcctgggt
    29161 atcgcccata aaagtcacgg tatcaccgag ggcaacgttt aagcgtttcg ccagcccctc
    29221 ttccatcgac acttcatcgg cttttggcgg ccagttaccg gcgacaatcg gattatgatc
    29281 gggccgcgta ttttgccagg taagattgag ttcgcgatta agcgcctcat cttcattacc
    29341 ttctgtcggc ttatcgttaa tcgccgtcag ccgcgcccgc actaccggat aaaatgattc
    29401 cgggactata tgatgttccg cgaggaacgc ttttagcggt gctacctgtt ctgtggcgat
    29461 gttaattaaa aagtagttcg ggctttctgg cggtagctgc tgctgccagc ggtcgagcag
    29521 atcgccacgc aataccagca acagcgccag cagcataaag gagagcgaaa atgccgaaag
    29581 ctggcttaac gttgaccacg gctgacgtaa caggcggcta accgccaggc gcagaggtag
    29641 cgatttcagc gtcatgcggc gaagtacatt cagctgcatc cagcccagca caccgcacag
    29701 caaagccagt actaccgcgc ccgccagcac cgcccaaagc agcatgctgc cacccattaa
    29761 tccggcgagc agcagcacaa ccaccacact gacaatcggc agataaaact tcagcggcca
    29821 gacgttcgct accacatcat tacgtaatac gcgtaaaggc tgcgttgcca gcaacaagcg
    29881 gtatggtcgt agccccacca gcagcgagat gaccgtcatg gtgccaagcg cccacagcca
    29941 cggccagagg ctggctggcg gtagtgcagc aggcagaacg ggcttgagca gcaccatcaa
    30001 catgttttcg aacaacaggc ctatggcccc accggtaacg gctgaaagcg tcagcaccat
    30061 caaccactga ccgacgatta gcttacgcag ttgcgctcgc cctgccccca gcgttttgag
    30121 gatcgccacc agatcgtagc gactgcgaca gtaatgattc atcgctaccg ccaccgctgc
    30181 cactgccagc agcaaggtca gaagcgccga aagcagcagg aactgttgcg agcgttccat
    30241 cgatcgcccc agcgcgcctt cgtcctgttc cagaccgtac cagcgttgtt cgggtttaag
    30301 ctgaggtaac aaccatttct catagccgtc gagctggttc tcgttgccgc cgaatttata
    30361 acgccaggtg acccgactcc ccggctgcac ggctccggtt ttatcgacat ccgccagatt
    30421 catcatcaga cgcggagcca tctggaaggg gttaaaaccg gaatccggtt cctgaatcac
    30481 ttctccggca atccgcaagg tggcatcgcc cacgtcaatg gtgtcgcccg ttttcaggtt
    30541 aagcagtgcc atcaggcgtg gggccagcaa tacgctgccc gcctgcggtt tcaggccagg
    30601 gggattagtt tgcagatcgc catacatcgg gtagatatca tccaccgctt tgacgttcgc
    30661 cagctgcggt gtgtcgcctg caaaggtcat ggtggcgaaa gtcagctgct tgccgacttt
    30721 caggccgcgc ttttgcgctt cctccagcca cgcttgcggc acttcgcgtg aactgcgcaa
    30781 cgcccgatcg cccgccataa actcacggct ttgctggctt aagcccttct ccatgcgatc
    30841 gctgatattg cccagcgcca gcacgcaggc caccgccagg cttagcgcca gccagacaat
    30901 taatagcgac ggcgaacgcc attcgcgcca gaaccaacgt gcaatcatgc ttcctcctgc
    30961 aactgcccgt tcaccagccg taagcagcag tcgcagcgtg ccgccagttg caggtcgtgg
    31021 gtcaccataa tcaacgtggt gccatgttca cggttgaggg aaaacagcag gtcggcaatt
    31081 ttatcgcccg tctggcggtc aaggttgccg gtgggttcgt cggcaaacag cacatcaggt
    31141 cgaccattaa aggctcgtgc cagcgccact cgttgctgtt caccgccgga aagctgtgcc
    31201 ggaagatgat ccagacgttt acccagccct aactgttcga gcaacgcttt cgccccgtta
    31261 cgactttccg cgctactctc accgcgcagc agagccggaa gctcgacgtt ttccagcgcg
    31321 ttaagggtag gaattaacat aaatgactga aaaacaaagc cgacgtgctt cgcgcgcaac
    31381 tttgcccgcg cttcttcgtc catattatgt agcggttgtc ccaccagact cacttcgcca
    31441 ctgctgccgt catcaagccc ggcgaggatc gccagcaagg ttgacttacc cgatcccgac
    31501 tcgcccacca gtgcgatggt ctcgccacgt ttgacaacca gctcaactcc ggtgaggatg
    31561 gagagttcat gctccccctg accaacggac ttcttaagat gatgaacttc aacaatgttt
    31621 tccgctggca tttgcccttc ctgttcctgg tcctgttaac cttccgtgcc gccgcagcgg
    31681 acacgttatt gattctgggt gatagcctga gcgccgggta tcgaatgtct gccagcgcgg
    31741 cctggcctgc cttgttgaat gataagtggc agagtaaaac gtcggtagtt aatgccagca
    31801 tcagcggcga cacctcgcaa caaggactgg cgcgccttcc ggctctgctg aaacagcatc
    31861 agccgcgctg ggtgctggtt gaactgggcg gcaatgacgg tttgcgtggt tttcagccac
    31921 agcaaaccga gcaaacgctg cgccagattt tgcaggatgt caaagccgcc aacgctgaac
    31981 cattgttaat gcaaatacgt ctgcctgcaa actatggtcg ccgttataat gaagccttta
    32041 gcgccattta ccccaaactc gccaaagagt ttgatgttcc gctgctgccc ttttttatgg
    32101 aagaggtcta cctcaagcca caatggatgc aggatgacgg tattcatccc aaccgcgacg
    32161 cccagccgtt tattgccgac tggatggcga agcagttgca gcctttagta aatcatgact
    32221 cataaagcaa cggagatcct gacaggtaaa gttatgcaaa aatcggtctt aattaccgga
    32281 tgttccagtg gaattggcct ggaaagcgcg ctcgaattaa aacgccaggg ttttcatgtg
    32341 ctggcaggtt gccgaaaacc ggatgatgtt gagcgtatga acaacatggg atttaccggc
    32401 gtgttgatcg atctggattc accagaaagt gttgatcgcg cagcagacga ggtgatcgcc
    32461 ctgaccgata attgtctgta tgggatcttt aacaatgccg gattcggcat gtatggcccc
    32521 ctttccacca tcagccgtgc gcagatggaa cagcagtttt ccgccaactt tttcggcgca
    32581 caccagctca ccatgcgcct gttacccgcg atgttaccgc acggtgaagg gcgtattgtg
    32641 atgacatcat cggtgatggg attaatctcc acgccgggtc gtggcgctta cgcggccagt
    32701 aaatatgcgc tggaggcgtg gtcagacgca ctgcgcatgg agctgcgcca cagcggaatt
    32761 aaagtcagcc tgatcgaacc cggtcccatt cgtactcgct tcaccgacaa cgtcaaccag
    32821 acgcaaagtg ataaaccagt cgaaaatccc ggcatcgccg cccgctttac gttgggaccg
    32881 gaagcggtgg tggacaaaat acgccatgct tttattagcg agaagccgaa gatgcgctat
    32941 ccggtaacgc tggtgacctg ggcagtaatg gtgctcaagc gcctgctgcc ggggcgcgtg
    33001 atggacaaaa tattgcaggg gtgagttgaa gcgcgcgctt aagcccccat gtcaaagaaa
    33061 atatcgacaa cagagagcga ctccatgtcc gtagaaaata ttgtcaacat taacgaatct
    33121 aacctgcaac aggttcttga acagtcgatg accactccgg tgctgttcta tttttggtct
    33181 gaacgtagcc agcactgttt gcagttaacc ccgatcctgg aaagcctcgc ggcgcagtac
    33241 aacgggcaat ttattctggc gaagctggac tgcgacgcgg agcagatgat tgccgcccag
    33301 tttggtctgc gcgcgattcc gaccgtgtat ctgttccaga acgggcaacc ggtagatggc
    33361 ttccaggggc cgcaaccgga agaggcgatc cgcgccctgc tggataaagt gctgccgcgc
    33421 gaagaagagc tgaaagcgca gcaggcgatg caactgatgc aggaaggcaa ttacaccgat
    33481 gccctgccat tgctgaaaga cgcctggcag ttgtcgaatc agaacgggga gatcggcctg
    33541 ctgctggcag aaacgctgat tgcgctgaac cgttctgaag atgcggaagc ggtgctgaaa
    33601 accattccgt tgcaggatca ggacacccgc taccaggggc tggtggcgca aatcgaactg
    33661 ctgaagcagg cggctgatac gccggaaatt caacagttgc aacagcaggt ggcggagaat
    33721 ccagaagatg ccgcactggc gacgcaactg gcgctgcaac tgcatcaggt tgggcgcaat
    33781 gaagaggcgc tggagttgct gttcgggcat ctgcgtaaag atctcaccgc cgcagacggt
    33841 cagacgcgta aaacgttcca ggagatcctc gctgcgctgg gtacgggtga tgcactggcg
    33901 tcgaagtatc gccgccagct gtatgcattg ttgtattgat attccgttga tcggcacatg
    33961 aaccacaacg ccggatgcga tgctgcgcat ccggcatcgc atcatttctt cttcaattgc
    34021 gtcaccacca actggtggcg cgaattataa aacttacgat aggttaaata gcaggcaata
    34081 atggtcgaca agctggcggt tgagagcagc ataaaggtca ccataatctg atatttaatc
    34141 gccttcaccg gatcaatccc ggcaaatatc agcccggaca tcattcctgg taaactcact
    34201 aagccaaccg tttttgctga atcgaccgtc ggaattaaag ccgcgcgaat actgtcgcga
    34261 atcaatatcg ctgaagcctg cttcggcgtc gcgccaagac tcagtttctc ctgaatctgt
    34321 tgctgttcgc taatgacccg ttgccctaaa ttgttgtaac acaaccccac cgctaccatg
    34381 gcgttaccgg caatcatccc ggcgataggg atcacctgca tcgggataaa ttcaatcgac
    34441 cctgagagaa tcagcaccgc cagggtaatt cccgccccga ccgtaatggc aataaacgat
    34501 gagataaaag ctttagcaat atatttactg cgtttttgcg cattccacgc cgcattaaag
    34561 cagataaata acaccatcaa taatgtcagg ctggcatcat ccacgctgaa aatatacttc
    34621 agcacatagc cgacaataat cagctgaatt atcgctcgcc cgacgctcca gagaatatct
    34681 ttctccagcg ccagtttttc tttatggcta attaagattg ccaccaccac cagcattaat
    34741 gccagtgcta atgattcgtt agtaatatta tgcgagttca tagcgtgctt cctgcatttc
    34801 tccggcatgc ggttgcagtg taatcacttt atccgcatga ttaatttcgt ctttatcgtg
    34861 tgtcacccac agcacggcaa tattttgctc gcgcacataa cgatggatca tctcattgac
    34921 gttatgttta ttactttcat ccagcgcact ggttatttca tccagcaata aaaccttcgg
    34981 cataaattgc aggttacgaa tcaatgagat gcgttgtttt tcaccaccag atagctcggc
    35041 gatatccttc gtcaaaatgc tgtccggcaa agcgaagcgt tcgagaaaat cgagaaaaat
    35101 ggccgggtca ggctgctggt tacggatctg ccagggaaag atcagattat cgtataccgt
    35161 atcgccaaac agcgtcggtg tctgggcgca gtaagagact tgctggcggt agatttctgg
    35221 ctttagtgtg ctgacatcct ccccttcaaa cagtaacgtt ccgctggttg ggctgatcaa
    35281 tgaagcaact atttttagca gcgtactttt gccacaacca gaaggaccgg taattaactt
    35341 aaattcgcca gcacgcagcg aaaaattgat gttattaaga atcttcgtat cacccgccag
    35401 atatcctacg ttttgtagct gaagcaaagg actattttcc tgcatcgctg ttcccttttt
    35461 ctgattttta ctaaaaacag tttatccttc gcaggaataa gggggaactc tctttcagta
    35521 atcaggtata atttgtttaa tcaagcagca attttgctga ttatggacgg tacaacagga
    35581 ggtttttccg atgcttatct ttatcccgat tctcattttt gtcgcgctgg tcattgtcgg
    35641 cgcgggcgtc aaaatcgtgc cgcagggcta tcaatggacg gtagaacgtt ttggtcgcta
    35701 taccaaaacg ttacagccgg ggctcagtct ggtggtgccg tttatggatc gcattggtcg
    35761 caagatcaat atgatggagc aagtgctcga tatcccttcc caggaagtta tctcgaaaga
    35821 taacgccaac gttaccatcg acgccgtgtg ctttattcag gtgattgacg cgccacgcgc
    35881 ggcttatgag gtcagcaatc tggagctggc tatcatcaac ctgaccatga ctaacatccg
    35941 taccgtgctg ggttcaatgg aacttgatga aatgctctct cagcgcgaca gcatcaactc
    36001 acgcctgctg cgtattgtcg atgaagccac caacccgtgg gggattaaag tcacccgtat
    36061 tgaaattcgc gacgtgcgcc caccggcaga gcttatctct tcaatgaacg cgcagatgaa
    36121 agcggaacgt accaaacgcg cttacatcct tgaagcggaa gggatccgtc aggcggaaat
    36181 cctcaaagcc gaaggtgaaa aacagtcgca aatcctgaaa gcggaaggcg aacgtcagtc
    36241 ggcgttttta caggctgaag cgcgtgaacg ttccgctgaa gcagaagccc gcgccaccaa
    36301 aatggtgtct gaagccatcg cctcaggtga tattcaggcg gtgaactact tcgtagcgca
    36361 gaaatacacc gaagcgttac agcagatcgg ttcgtccagt aacagcaaag tggtgatgat
    36421 gccattagag gccagcagcc tgatggggtc gattgccggg attgccgagc tggtgaaaga
    36481 cagcgccaac aaacggactc agccatgatg gagttaatgg tcgttcatcc acatattttc
    36541 tggctcagtc tcggcggttt gctgctggca gccgagatgc tgggcggaaa tggttatttg
    36601 ttgtggagcg gcgtggcagc ggtgattacc ggcctggtgg tctggctggt gccgctgggt
    36661 tgggagtggc aaggggtaat gtttgccatc ctgacgctgc tcgccgcctg gctgtggtgg
    36721 aaatggttgt cgcggcgggt gcgcgaacaa aagcacagcg atagtcattt aaaccagcgc
    36781 gggcagcagc tgattggccg acgttttgtg ctggaatctc cgctggtcaa cgggcgcggt
    36841 catatgcgcg tcggtgacag ttcatggcct gtcagcgcca gcgaggatct cggtgcaggt
    36901 acgcatgttg aagtcattgc gatagaaggg ataaccctac atatccgagc cgtctcgtct
    36961 taatcaccct gcccgatgat gacagcagcc ggagagattt tcgataatcg ggcagtcggc
    37021 gctgtcatcg ccagggcagg cattcgccag tgccagcagc tggtcgcgca tggattgcag
    37081 ctcctcaatg tgtcgttcga tctccgccac cttctccagc gtgcgccgtt tgacgtcggc
    37141 gctgtgccgc tgcgggtcgt taaacagatt caccagctcg ccgctctctt ccaggttaaa
    37201 gcccacctgc cgtgcctggc gcagtaaggt cagttcgttg agatgctgct gcgtgtaggt
    37261 gcgataaccg ttttcgctgc gcatcggcgg cgtcaccagc cccttctctt catagaagcg
    37321 aatggctttg ctggtcaggc cggtaatttt tgctacatcg ctgatgttca tcgttcgcgc
    37381 aacgcctcct gtgctttgtt aaagggtttc aacagatatt caagcacgtt tttttgccag
    37441 tgcgaatatc gaccgccacc acaataatac aggaagtact gcgcacagag agatgttata
    37501 cggttttatt gcgcttcatg accattgcca caataaggct gagtaagagt gagccaataa
    37561 tccatatcaa cgcacggcta ccctgggacc acagactgta gataaagcca acgatcacca
    37621 acaacatcac caacgtacca acgataagaa tcggcaacga ggcgtgaata tcatgacgca
    37681 ggcggatcgc caccacaaac accgccaggt agcaaatcaa aaaggtggcg ctggcaacac
    37741 tggcgagtga gcctaaattt agcgccgccg tcatcagcat aatcaacacc acgacaataa
    37801 tgttgcccca ggtactctgc cgccacaggg atttattcat tagcttcggc agttcgcgtt
    37861 cgctgcccat gttgtccatg atgttaaaca cggcgaacag gttcgcgtta atggctgaag
    37921 ccgtcgccag taaagcgccg ataacgacga tcacataacc cacatgcccg agcagcggag
    37981 aagcagcctg cgctacggcg gtatcggcat atttttctaa ctctaatgcc gatacatcgc
    38041 taagcaaaac cagtgccagc gagatataaa gcaacgtggt aacgccaatc gccaccagaa
    38101 acgcccgtgg cataatgacc tgcggatctt tcactttatc cgccgcgttc gccatcatgc
    38161 caaagcccgc ataggcaagg aaagttatcc caatacagga gaagaacgca ccggagctgg
    38221 ggggcgcaga gacggaaata tgcgctggtt gcagcgacca gacaccggca ataatcagca
    38281 ataacaggat catcatttta atgccgacga ggatcacttc cagccgccct accgcatggt
    38341 tgcttaagga gttgaaaagc gtcatcaccg caatgatccc caacgcgtag agcaaaataa
    38401 ggtgctcctc ctggctgcct tcatgcaaaa actgcacggc ataagcgcca aaagcacggg
    38461 cgaccatggc gatgcttacc gccagcgtca acaggtacag taacgagagc gccagcgaaa
    38521 agacgccgtt gcctaatccg cgacgaaaga agtcgataat gccgccattg ctgggatagc
    38581 tcgcccccag acgcgcatag gcataaccgg aaaacatcgc cacaataccg ccaaaagcaa
    38641 aggcgaccca ggtcgaggct tccattagca atgcagcctg ccccagcagc gcgaa
//
LOCUS       DAOFQD010000031        38670 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.031, whole genome shotgun sequence.
ACCESSION   DAOFQD010000031 DAOFQD010000000
VERSION     DAOFQD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.031"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            101..1762
                     /locus_tag="QBF46_001681"
     CDS             101..1762
                     /locus_tag="QBF46_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDQ6673873.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYHRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVENKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDASTGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLTALTSPLTSTTPVAENVE"
     gene            2001..2060
                     /locus_tag="QBF46_001682"
     CDS             2001..2060
                     /locus_tag="QBF46_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDQ6673874.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2376..2435
                     /gene="ibsE"
                     /locus_tag="QBF46_001683"
     CDS             2376..2435
                     /gene="ibsE"
                     /locus_tag="QBF46_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDQ6673875.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2555..3988)
                     /gene="hldE"
                     /locus_tag="QBF46_001684"
     CDS             complement(2555..3988)
                     /gene="hldE"
                     /locus_tag="QBF46_001684"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDQ6673876.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGAYARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4036..6876)
                     /gene="glnE"
                     /locus_tag="QBF46_001685"
     CDS             complement(4036..6876)
                     /gene="glnE"
                     /locus_tag="QBF46_001685"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDQ6673877.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(6899..8200)
                     /gene="ygiF"
                     /locus_tag="QBF46_001686"
     CDS             complement(6899..8200)
                     /gene="ygiF"
                     /locus_tag="QBF46_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDQ6673878.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRLGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8442..9062
                     /locus_tag="QBF46_001687"
     CDS             8442..9062
                     /locus_tag="QBF46_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDQ6673879.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9126..10364
                     /gene="cca"
                     /locus_tag="QBF46_001688"
     CDS             9126..10364
                     /gene="cca"
                     /locus_tag="QBF46_001688"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDQ6673880.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(10545..11366)
                     /gene="bacA"
                     /locus_tag="QBF46_001689"
     CDS             complement(10545..11366)
                     /gene="bacA"
                     /locus_tag="QBF46_001689"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDQ6673881.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11456..11824)
                     /gene="folB"
                     /locus_tag="QBF46_001690"
     CDS             complement(11456..11824)
                     /gene="folB"
                     /locus_tag="QBF46_001690"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDQ6673882.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11929..12546
                     /gene="plsY"
                     /locus_tag="QBF46_001691"
     CDS             11929..12546
                     /gene="plsY"
                     /locus_tag="QBF46_001691"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDQ6673883.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12559..13491)
                     /gene="ttdR"
                     /locus_tag="QBF46_001692"
                     /gene_synonym="dan"
     CDS             complement(12559..13491)
                     /gene="ttdR"
                     /locus_tag="QBF46_001692"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDQ6673884.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13698..14609
                     /gene="ttdA"
                     /locus_tag="QBF46_001693"
     CDS             13698..14609
                     /gene="ttdA"
                     /locus_tag="QBF46_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDQ6673885.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14606..15211
                     /gene="ttdB"
                     /locus_tag="QBF46_001694"
     CDS             14606..15211
                     /gene="ttdB"
                     /locus_tag="QBF46_001694"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDQ6673886.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15259..16722
                     /gene="ttdT"
                     /locus_tag="QBF46_001695"
     CDS             15259..16722
                     /gene="ttdT"
                     /locus_tag="QBF46_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDQ6673887.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTLVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16765..17778)
                     /gene="tsaD"
                     /locus_tag="QBF46_001696"
     CDS             complement(16765..17778)
                     /gene="tsaD"
                     /locus_tag="QBF46_001696"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDQ6673888.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18016..18231
                     /gene="rpsU"
                     /locus_tag="QBF46_001697"
     CDS             18016..18231
                     /gene="rpsU"
                     /locus_tag="QBF46_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDQ6673889.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18342..20087
                     /gene="dnaG"
                     /locus_tag="QBF46_001698"
     CDS             18342..20087
                     /gene="dnaG"
                     /locus_tag="QBF46_001698"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDQ6673890.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20282..22123
                     /gene="rpoD"
                     /locus_tag="QBF46_001699"
     CDS             20282..22123
                     /gene="rpoD"
                     /locus_tag="QBF46_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDQ6673891.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22202..22708)
                     /gene="mug"
                     /locus_tag="QBF46_001700"
     CDS             complement(22202..22708)
                     /gene="mug"
                     /locus_tag="QBF46_001700"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDQ6673892.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22833..22908
                     /locus_tag="QBF46_001701"
     tRNA            22833..22908
                     /locus_tag="QBF46_001701"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22866..22868,aa:Ile,seq:cat)
     gene            complement(22962..23726)
                     /gene="nfeF"
                     /locus_tag="QBF46_001702"
     CDS             complement(22962..23726)
                     /gene="nfeF"
                     /locus_tag="QBF46_001702"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDQ6673893.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHCKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            24014..24637
                     /gene="nfeR"
                     /locus_tag="QBF46_001703"
                     /gene_synonym="yqjI"
     CDS             24014..24637
                     /gene="nfeR"
                     /locus_tag="QBF46_001703"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDQ6673894.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(24791..26311)
                     /gene="aer"
                     /locus_tag="QBF46_001704"
     CDS             complement(24791..26311)
                     /gene="aer"
                     /locus_tag="QBF46_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDQ6673895.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            26729..28108
                     /gene="ygjG"
                     /locus_tag="QBF46_001705"
     CDS             26729..28108
                     /gene="ygjG"
                     /locus_tag="QBF46_001705"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDQ6673896.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(28150..28482)
                     /locus_tag="QBF46_001706"
     CDS             complement(28150..28482)
                     /locus_tag="QBF46_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDQ6673897.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            28701..29684
                     /gene="ebgR"
                     /locus_tag="QBF46_001707"
     CDS             28701..29684
                     /gene="ebgR"
                     /locus_tag="QBF46_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDQ6673898.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29868..32960
                     /gene="ebgA"
                     /locus_tag="QBF46_001708"
     CDS             29868..32960
                     /gene="ebgA"
                     /locus_tag="QBF46_001708"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDQ6673899.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFTRETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHASQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            32957..33406
                     /locus_tag="QBF46_001709"
     CDS             32957..33406
                     /locus_tag="QBF46_001709"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDQ6673900.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            33469..34902
                     /locus_tag="QBF46_001710"
     CDS             33469..34902
                     /locus_tag="QBF46_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6673901.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            35036..36106
                     /gene="ygjJ"
                     /locus_tag="QBF46_001711"
     CDS             35036..36106
                     /gene="ygjJ"
                     /locus_tag="QBF46_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDQ6673902.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            36123..38474
                     /gene="ygjK"
                     /locus_tag="QBF46_001712"
     CDS             36123..38474
                     /gene="ygjK"
                     /locus_tag="QBF46_001712"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDQ6673903.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
BASE COUNT         9355 a        10525 c         9917 g         8873 t
ORIGIN      
        1 aagtgttaat tatttgccga ctttctgcaa cgcgatattt ggcggaaaat aatccctggc
       61 cccaaatatt gtaatttatt aatcaaaagg aaatttaata atggatgata ttgttaattc
      121 tgtgccctcc tggatgttta ccgcaattat tgccgtatgc attctgttta ttattggaat
      181 tattttcgcc aggctctatc atcgcgcttc ggcagagcaa gcttttgttc gtactggttt
      241 aggtgggcaa aaagtggtaa tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat
      301 aatccccatc aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct
      361 gattacgaaa gatcgtatgc gcgtcgatgt agtagtcgct ttctttgtgc gggtaaaacc
      421 ttcagtagaa gggattgcca ccgctgccca gacgctgggg caacgcaccc tgtcgcctga
      481 agacttacgt atgttggttg aaaataaatt tgtcgatgcc ctccgtgcaa cagctgcgca
      541 aatgaccatg catgagttac aggatacccg cgagaacttc gtgcaggggg tgcaaaatac
      601 agtggcagaa gacctgtcga aaaacggtct ggaactggag agcgtttcac ttaccaactt
      661 taaccagacc tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac
      721 caaactgact caggaaacag agcgccgtcg ccgcgaacgt aacgaagttg aacaggatgt
      781 agaagttgcg gtgcgtgaaa aaaatcgcga tgcgctatcg cgcaagctgg agattgaaca
      841 gcaagaagcg tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca
      901 gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacacgaat
      961 tctggctgaa cgacagattc aggaaacaga aatcgaccgc gaacaggccg tccgctcaag
     1021 aaaggttgaa gctgaacgtg aagttcgcat taaagagatc gaacagcagc aggtcaccga
     1081 aatcgctaac cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc
     1141 agaagcgcgt gctaatctcg cacttgcaga agcggtaagc gcccaacaaa acgtagaaac
     1201 cactcgccag actgccgaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca
     1261 ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc
     1321 agagatgcag gcggcggcta tcgttgagtt agccgaagct acacgtaaaa agggtctggc
     1381 ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac
     1441 cagccttaaa ttcaaactgg ccttgttgca ggcgctgcct gcggtaatag agaaatccgt
     1501 tgagccgatg aagtcaatcg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg
     1561 cggcgctgcg ggtgatgcga gtacaggtag cgttagtgga ggaaaccttg cagagcaggc
     1621 attgtctgcc gccctttctt accgcacaca ggcaccgctg attgactcct tgctcaatga
     1681 aattggtgtt tcaggcggct cactgacggc attgacttca cccttaacct caacaactcc
     1741 cgtcgccgaa aacgtagaat aaattttcag ctatgaacgt ccctggcaca aaaagccggg
     1801 gattttttat atctgcgttc cgctaaaaag tgcaaatgat caggttgttg cagcattttg
     1861 cgtgacggct cggggaaggc aaaactgcat cagagaaagc attgcgtggg gtctggcgct
     1921 catcaacaat cggggagtca gcagggggct gaaacgggaa agcccctccc gaagaagggg
     1981 ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttactcg
     2041 taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt
     2101 tcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct
     2161 ggcccaaaaa gccggggatt ttttatatct gcgttccact aaaaggtgca aatgctcagg
     2221 acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ttgcctctgg gaaagcattg
     2281 cgcggggtcc ggcgctcatc aacaatcggg gggcagcaag gggctgaaac gggaaagccc
     2341 ctcccgaaga aggggccttg tataaggaaa gggttatgat gaagttcgtc atcatactgg
     2401 ttgtgttgtt actgttaagt ttcccgactt actaacaact catcagaggg gggagaaatc
     2461 ctcccttacc cttgttcctt tactctaggt tgaaaaaaca acagcgtcaa taggcctgcc
     2521 atgtacgaag cgagatctgt gaaccgcttt ccggttagcc ttttttatcc tgttggatct
     2581 tcttgatgat gttggtcgtc gagcaaccgt cttcaaagtt gagcaccaac acttcgccac
     2641 cgttggccca gacttcttta ctcccggcaa tctcttctgg tttatagtcg ccgcctttca
     2701 ccagcagatc tggcaagatc ccggcgatca agcgctgcgg cgtgtcctct tcaaacgaca
     2761 ccacccagtc gaccgcttcc agtgcgccca gcacaatcat acgctgttcg agcgggttaa
     2821 cggggcggga atcccctttc agccgtttgg tggaggcatc gctgttcaca gcgacaatca
     2881 aacggtcacc cagtttacgg gcatttgcca gataagagac gtgcccggcg tgcaggatgt
     2941 caaagacacc gttggtcatc accacttttt caccacgttt acgcgctgcc gctacggcca
     3001 gcttcagttc ctcttcggtc atcacgccaa agcctgtatc tgcacgtcca cgtacagcat
     3061 tttccagctc gatcggcgaa accgtggagg ttcccagttt gccgaccacc acgccagccg
     3121 ccgcattggc aaagaagcag gcttcttcca gcgaattacc cgctgccagc gttgccgcca
     3181 ggacgccaat caccgtgtcg cccgcaccgg taacgtcata cacttcctgc gcctgggttg
     3241 gcatatgcag cggcgcttta cccggttgca gcagcgacat accctgttcg gaacgggtca
     3301 ctaacagagc cgagagttcg taatcggcaa tcagtttcat gccgcgctca acaatctctt
     3361 cttcggtctt acatttaccc acaacagctt caaattccga gagattcggc gttaacagcg
     3421 tagcgccgcg gtagcgctca aaatcggtac cttttggatc aatcagcacc ggaacacccg
     3481 ctttacgcgc cagttggatc atctgctgta cgcttgccag cgcacctttg gcgtagtcag
     3541 aaagcaccag cgcgccaatc gaactcagcg cctgattaat ccgctcgtgc agcggctgcg
     3601 gatcaacacc ttcgaaacct tcttcaaaat ccagacggat cagctgttgg ttgcgggaaa
     3661 gtacccgtaa tttggtaatg gtcggatgcg tcggtacaga aacgaagtcg catttgacgt
     3721 tgacgtcggc cagagattta ctcagcgcgc gcgctgcatc gtcaatgccc gtcaacccaa
     3781 ccaggcgtgc ataagcaccg agagaagcga tattcatcgc cacgttagcc gcaccgcccg
     3841 gacgttcttc gatggtattc actttaacca cgggcaccgg cgcttccggc gagatacgac
     3901 tggtggggcc gtaccagtaa cgatccagca tcacatcacc aaccaccatc actcctgcac
     3961 gttcaaactc tggcagcgtt actttcattc ctgtctcctg agagattcaa aatttgcgcg
     4021 cgataatacc atacttcatt cttccaccag ccacttctgc cagcttgccc gcaccagttc
     4081 acgctctgcg gtgaagcaat cctccgacac atggcccggc aattcctgta atgccagatg
     4141 atgaagttca tcgcgaagcg tagtgtaagc acgggtcagc gccatcgctt cctgctcttc
     4201 cataatgtcg ttttgcgcca gtagttccag aatacgcacg ttgtctgacc agcgcgttaa
     4261 cttcggtttt tcatgagcgt agcgcaacac cagatattgg gtaataaatt cgatatcggt
     4321 aattccccct tcatcagctt tgatatcaaa gcgatcgcga tgtttattgc cgagatgagc
     4381 gcgcattttc tcgcgcattt cccgcacttc cgtttgcaga gttttacctt cacgcggcag
     4441 cgtcataatc tcgcgacgca ctgcgtcaaa gtgcgcggtg agctgcggat cgccgtacac
     4501 tacacgcgca cgcaccagcg cctgatgttc ccacgtccag gcctcgtttt tctgataatc
     4561 ggcaaatgct tctgcggatg tcaccagcat tcccgccgcc ccggacggac gcagtcgagc
     4621 atccacttca tacaaaatgc cggaagaggt acgcgtactg aacagatgca taatgcgttg
     4681 cgccagacgc agataaaact gccgcccgtc gatttcccgc tcaccgtcag tcatcgcatc
     4741 cattgggcaa tcatggagga agataaggtc aagatcggaa ctgtagccta actcccagcc
     4801 gcccagcttg ccgtagccga ccaccgcaaa accacgccct tcgcgttcgt tcaggtgatt
     4861 cggcttaccg tagcgggcaa ccatttgaac ccacgcctgc tgaacgacgg catctatcat
     4921 ggcttccgcc agccaggtta agtgatcgct cactttcatc accggtagcg taccggcgat
     4981 atccgctgcg gcgatgcgta acagctgcgc ctgtttgaac tgacgcagcg cctcaagctg
     5041 ttgctcttcg tcatcttccg gcacgcgcag caaatactgg cgcaactcat cgcggtaggc
     5101 atcggtcgcc gtcggctggt aaagggtgtt tggatcgagc aattcatcca gcaataatgg
     5161 ataacgcgcc agctggctgg caatcatcgg cgacgcggca cacagagaaa tcaaatgttt
     5221 aagcgccgcg gggaattcac tgagcaattc taaataggtg gtgcgggtaa caatccccac
     5281 cagcaaggcg gtaatgcgcg acagcgtaac ggcagcgtct tcacgcgcac agacatcact
     5341 tagcagatgc ggcatcagat ggtcgagcac ctgacgtcct cgcggcccga tggtgcgctt
     5401 atccagctct ttgcggaaat cggcaatcag cgttagcacc tgtttgcgat catcctcgct
     5461 aagatgcgcc agcactggcg tagtgtcatc ttcctgcaac gcatcctgcc acagctcacg
     5521 ccactgttcc gacagcgact cttcctgagt ttcactttcg tcgtcgccaa tcaattcatt
     5581 aaacacccgg cgcacattgg tcatatgtgc ggtcagcgcc ccggtcaatt gcggccagtc
     5641 agcaaagtcc atcgcccacg ccagccgcgc acgattaagc tcatcagaag gaagcgtctg
     5701 ggtttgttcg tcgttaatgc tttgcagcag gttttccaga cgccgcagga acagatacgc
     5761 cactcgcaat tgttcagcat cgttttcaga aagcagatgc agctcggcaa tggcgctgag
     5821 cgttggcagt aaagagcgcg attgcagcga cggttcgcgt ccgccgcgaa tgagctggaa
     5881 cacctgaacg ataaattcaa tttcgcgaat gccgcctgcg ccgagtttga tattgtcggt
     5941 caaaccacgt cgacgcactt cacgggcaat catccctttc atgttgcgca gcgactgaat
     6001 cacgctgaaa tcgatgtaac gacggaaaac aaacgggcgc agcatcgcac gcaactcgtt
     6061 agcatagacg ccttcgctat cgcccataat ccgcgccttg accatcgcgt aacgctccca
     6121 gtcgcgcccc tgctcctggt aataatcttc caacgcggca aagctcagca ccagcgggcc
     6181 actttcgcca aacggacgca gccgcatatc cacgcgatag acgaagccat cctgcgttgg
     6241 ttgatccagc actttaatca gccgctgccc catgcgggta aaaaactgcg cgttatccag
     6301 ttcccggcgt ccaccctgcg tacaaccatg ttccggccag gcaaaaatca gatcgatatc
     6361 agaggagaaa ttcagttccc cgccgcccag cttacccatg cctaaaatca gcagcggttg
     6421 cgcttcgccc tgcgcattgc acggcgttcc ccactcgcgg cagcaggcgt catatagcca
     6481 gtcacgcgcc gcaacaatca gcgtctccgc cagatagctg agctgctgca atatgctctc
     6541 ttcagtaacc agtgccagcg tttgcgccca ggcgatacgt accatgatgc gccgacggaa
     6601 cagccgcagc tcgcgcatta acccggcttc gtcactcaca ttactgagcg cctcctgcaa
     6661 ccatgcaacg taatgctgcc attcgtcggc ctgcggcggt tggctttcca gttccgtcag
     6721 ccactctgga tgcgcactta tgctgtcctg cacaaaatca ctgaaagtaa gtactgactt
     6781 cgcctgtgca ctaagtgatt cctcggctaa aggctctggc agccgctcaa caacggtctg
     6841 ccagtactgc tgtaacggtg aagagagcgg cttcataagc gattttatcc ttgcctgatt
     6901 aacgttttcc gctgtgcaac cagaacggtt cctgattgtt tgcctcatta cggaaatgtt
     6961 caatttcgat gcgttgcccg gtcgcaatag cgtgatgcag cccctgccag ttctccagcc
     7021 aggcttgcgc gacgacagga tcatagtaac ccgccagcaa cagtattgag tcaatatcac
     7081 gcgtcaggcg tggcaactgg tcacggtagc gatcgcctaa cggctggcag aaaacgcttt
     7141 tcagttcagc ggcatggcgg gaaagatgga tatcggcaaa gcgtttgaag gagtcgctga
     7201 ttttgccctg cgctttggca tctaaaaatg gctgccatgc tttgcttacc aaccattcgg
     7261 tcaacgccag cttcgccatt gccgtttcgg tagagtagac cgccgtcacg gcagacaccg
     7321 cagaagcaat ggtcgcctcg cattgagtca gcagatcacg taagtgagtg ctcgctttac
     7381 gcggcacaat accaccgaac agcatcagcg tatgacggac caggctaatg gctgccagca
     7441 cctgttcttt cgccgcatcg ttgccgcgta cccacagttc ttcatgatac tgccattgcg
     7501 ctaacgccag ctcgagcgcc gcttccagcc cctgttccac atcggctttt gccgcaacat
     7561 gcaaaatggt ggtcggtttg atttcacgcg ccggattgcc ctgcgccaga tgatagccac
     7621 gcgccgcttt gctcaggctg cccaggcgta atccggtttg cgataccagt tggttcgcca
     7681 gtttcagcac cgcgcgcgtg tcgccgctaa gcagttccag ttccagctca cagataggtt
     7741 cagcaaattc acccgctttc acttccccct ggtcgagggc gatttcaatt tggctaccat
     7801 cgaccgccac cagccatttt tcgcgataaa aatcggtgct gaacagcggc tgcacgcggg
     7861 aggcgagatc ggcgggcaat tcgccgttcg gccagacttc cgtcggtaac tgcgccaggt
     7921 cgagcgtcgg ttcgctcaac gccacgttat attccgggcg ctgatgtaag ccgcctgtca
     7981 ctcttcctgc aactttcatg gtcatctcat agcgaccgtt ttcgccacga atacgtaagc
     8041 ccatatcgtg cccacgcagc cagttatccg gcgtttcgta gtaaatattc agcaactgca
     8101 cggggtcatg gtgctcgccg cccagcgtat tgagatggtc acgcaacgcc tcaacggcac
     8161 tgtgattaac aataaacttt aattcgattt cctgagccat ggcctttact tatcggttat
     8221 gtcacataag tgacgatgaa cggcgaactt aatgcgatct tttttgtcag tagatagtat
     8281 tttgcgccaa attgccatgc aacgggcaat ttgacgggcg taaaagtttg aagcagtggc
     8341 aagacctgac acagaggatt ccgattgatg tcgaatgctt tgcgtagcga cactgatgcc
     8401 actactatcg ttccactttc catgacaata acgacagcct gatgccaaaa ttacgcctga
     8461 tcggattaac tttactcgca cttagcgcga ctgccgtctc acacgctgaa gaaacgcgct
     8521 atgtttccga cgaactgaat acctgggtcc gcagcggtcc gggagatcat tatcgcctcg
     8581 tgggcacggt taacgccggc gaggaagtga ccttattaca aactgacgcc aacaccaatt
     8641 atgcccaggt gaaagacagc tctggccgta ccgcctggat cccgttgaaa caacttagca
     8701 ctgagccaag cctgcgctcc cgtgtgccag atctggaaaa tcaggtcaaa accctgaccg
     8761 ataaactcac caatatcgat aacacctgga atcagcgcac cgcagaaatg cagcaaaaag
     8821 tggcgcagag cgacagcgtg atcaacgggt taaaagaaga aaatcagaaa ctgaaaaacg
     8881 agctgattgt cgcgcagaaa aaggtcgatg ccgccagcgt acagctggat gacaaacagc
     8941 gcaccatcat catgcaatgg tttatgtatg gcggtggcgt gctggggctt ggcttgctgc
     9001 tcggtctggt actgccgcac ctgatcccaa gccgcaaacg caaagatcgc tggatgaact
     9061 aaatcgcctt cttcgttgca ctgtcttatt attaggaatt gtgtatttga cgaagggatg
     9121 atggcgtgaa gatttatctg gtcggtggtg ctgttcggga tgcattgtta gggctaccgg
     9181 tcaaagacag agattgggtg gtggtcggca gtacgccaca ggagatgctc gacgcgggct
     9241 accagcaggt aggccgcgat tttcctgtgt ttctgcatcc gcaaacgcat gaagagtatg
     9301 cgctggcacg taccgaacgg aaatccggtt ccggttacac cggttttact tgctatgccg
     9361 caccggatgt cacgctggaa gatgatctta agcgtcgcga tctgaccatt aatgcgctgg
     9421 cccaggacga taacggcgag attatcgacc cgtacaacgg tctgggcgat ctgcaaaatc
     9481 gtctgttgcg ccatgtttcc cccgcttttg gcgaagatcc gttacgcgta ttgcgcgtgg
     9541 cgcgttttgc tgcgcgttat gcccacctcg gttttcgtat tgccgatgaa actctggcgt
     9601 tgatgcgcga gatgacccat gcgggtgaac tggaacacct gacgcctgaa cgggtatgga
     9661 aagagacgga aagcgccctc accacccgca atccacaggt gttcttccag gtactgcgcg
     9721 attgcggcgc actgcgcgtt ttattcccgg aaattgacgc actgtttggc gttccggccc
     9781 ctgccaagtg gcatccggaa atcgatacgg gtattcatac cttaatgacg ctctcaatgg
     9841 cggcgatgct gagtccgcag gtcgatgtcc gtttcgcaac tttatgccac gatctcggta
     9901 aagggctgac gccgccagaa ctctggccgc gtcatcatgg tcatggcccg gcgggtgtta
     9961 agttagtgga acaactatgc cagcgtctgc gcgtgccaaa tgaaattcgc gatttagcca
    10021 gactggtggc tgagtttcac gatctcatcc acaccttccc aatgctgaac ccaaaaacca
    10081 tcgtcaaatt atttgattcc atcgatgcct ggcgtaaacc gcagcgtgtc gagcaactgg
    10141 cgctgaccag cgaggctgac gtgcgcggca gaaccggttt tgaatcagcg gactacccgc
    10201 aaggccgctg gttgcgcgaa gcctgggaag tggcgcagtc agtgccgaca aaagccgtcg
    10261 ttgaagcggg atttaaaggt gtggagattc gcgaggagct gacccgacgg cggattgcgg
    10321 cggtagccag ctggaaggaa caacgttgcc caaagcctga atgagtattg ggcattttgc
    10381 cggatgcggc gtaaacgcct tatccggcta catgtcaacg acagttgtag gcctgataag
    10441 acgcgcaagc gtcgcatcag gcgttgattg ccggatgcgg cgtgaacgcc ttattcggcc
    10501 tacgcaatcg ttgacaacgc caagcatccg acactattcc tcaattaaaa gaacacgaca
    10561 tacaccgcag ccgccacaat aaagcgataa atggcgaacg ggataaacga aatgcgctta
    10621 atcaattgca ggaaggtttt aatcgctatc agcgccacca caaaagcggt gataaaccca
    10681 acggcaaaca tcgggatatc gccgcttgtc aggaagcccc agcttttgta gagatcgagc
    10741 gccgttgcgc ccatcatcat cggcaccgcc agcaggaacg aaaactcgga agcagcgtaa
    10801 cggctcaccc ccatcagcat cccacctgaa atggtcgccc cggaacggga gaaacccggc
    10861 cacagcgcca gacactggaa acagccaatc ataaatgcct gacgataggt catatcatca
    10921 agacccggcg cacgcggctc tttcggcttc aggcattcgg cggcaatcag caacaaaccg
    10981 ccaacgacca gcgcatacat cacatttatc gggttaaaca atgacttaat cgtgtcgtgg
    11041 aacaacagcc ccaataccac cgccggaatc atccccagca aaatgtggat cagcgttaaa
    11101 cgacctttgc tttcaccttc gtgctgcaac gggcggccaa agtggatgcc aatcaggcca
    11161 aacagacgcc gccagaacat cactactacc gccagaattg atcctaactg gatcacaact
    11221 tcaaaggttt tcgccgtgtc gccctcaaac cccaacaagt gaccgacaat aatcatatgg
    11281 cccgtgctgg ataccggcag aaattctgtc aatccttcga ccacacccaa tattgccgct
    11341 atcagcagcg agtgcatatc gctcatcaat aaacccctaa attattaaaa tgtaccgctt
    11401 gtccgaacta ctgcgtatga ccaggttata accgtttggt ttaacagctg taaaattaat
    11461 tattttcttt cagattattg ccacgctcaa tgattacgcc aacattcgcc gcccgcgcca
    11521 ctgcgcctgg cttgctgagt ttgatacgca cccacggcga gttgaagcgt gctaacagca
    11581 gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa acgcgccccc tcgacgtggc
    11641 tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc cgccacatca tcacttttcg
    11701 ccgctttacg gttatcccat gccatttcga tatcgaacac taacttctgt tcgatggtct
    11761 gttcccagtc gtaaacacca atagtggtga ttaccgaaag ttgctctata aatacaatat
    11821 ccatcacgtc ctgcctgctt tttggctaac cggataccac ttccggcgaa atgtgcgtat
    11881 tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg aacagcttat gagtgcaatc
    11941 gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca tttccagtgc cattctggtt
    12001 tgccgcttgt gtgggctgcc cgatccgcga accagcggct ccggcaatcc aggcgcaacc
    12061 aatgtgttac gtatcggtgg caagggagca gccgtagcag tactgatttt cgacgttctg
    12121 aaaggaatgt tgcccgtctg gggcgcgtat gaattaggtg tcagcccctt ctggctaggc
    12181 ttaattgcca tcgccgcctg tcttggacac atctggcccg ttttcttcgg atttaaagga
    12241 ggaaaaggcg ttgctaccgc ttttggtgcc atcgcaccca ttggctggga tctcaccgga
    12301 gtaatggcgg gaacctggtt actgaccgtg ctattgagcg gatactcgtc gctgggagcg
    12361 attgtcagtg cactgattgc tccgttttat gtctggtggt ttaagccaca attcaccttc
    12421 ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc atgacaacat ccagcgtctg
    12481 tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa gaaagcgcga aaaggatccc
    12541 gagtgatttc tggtggatct acatgacctg atagccttca tcgggcttgc ccagccgttg
    12601 ctggcaccat gccgccagaa attccacgca gacacgtaat ttcatgctgc gatagagcgg
    12661 ctcccggtaa acagcccaga tattggcgct ttgtgcatac tccggcaata cttgcaccag
    12721 tttgccactc tccagaaacg gcaacacatc ccactcggaa cgcagcataa tccctttgcc
    12781 ctccagcgcc cattgcaaca ctatctcgcc gctattggag gaaagatgcc cgctaacctt
    12841 caccgatttt ttctcctgac cgttccccaa ctcccatatt ccgtgggtca tatcgcgctc
    12901 tttggtcacc agacagtcat gacgacttaa ttcctgtaat gattgtggct gcggatattt
    12961 ttgcagatac tcaggtgctg cgcataatat tcttttattt ttcgttaaca gatgcgcaat
    13021 ataataatca ggaatttcgt cattaatacg aatatccaga tcaatattat cctgcactaa
    13081 atcaatttgc cgatcgaaca attcaaaatg cacctgtaac tcaggataat tgcgcataag
    13141 ttcggtaatg gctggcgcaa tatggctgcg cccaaaacca aaactacagc caatacgaat
    13201 catcccctcc gggcgcgttt tgatttgcgt gacgtcatcg accagtcgct gatattgggt
    13261 gaggatttcc agcgcatgtt cgtaacaacg ttggccactt tcagtcagcg ccacgccgcg
    13321 agcggagcgg ttcagcagcg ttgttgccag cgtattttca aggatttgga tccgcttggt
    13381 gacaaatgca ggcgtctgcc caagcgtcgc cgctgcggcg ctaaaactcc cgctgtggac
    13441 gatttctacc agtacctgca agtctttggc taaaggccag ctattcagca tcgttaatta
    13501 tccgcagttg tgataagcgc agtgtatttc gcaaaacatt gccattccct gctctggcgc
    13561 aatattcatt cttattttcg cgttgattca cgaactgtta attacatatt cacggtggca
    13621 aaaaatataa aaccacactt ttagtggtag tttgtggcgg tgaatttttc cagacaaata
    13681 caaaaactgg agttgccatg atgagcgaaa gtaataagca acaggcagtg aataagttga
    13741 cagagattgt cgctaacttt accgccatga tttctacccg aatgcctgat gacgtggtgg
    13801 ataaactaaa acagctaaag gatgccgaaa cgtcgtcgat ggggaaaatt atctaccata
    13861 cgatgttcga caacatgcaa aaagcgattg acctgaatcg tcctgcctgt caggacaccg
    13921 gggagattat gttcttcgtt aaagtcggtt cccgcttccc actgcttggc gagctgcaaa
    13981 gcatactcaa acaagccgtg gaagaggcaa ccgtcaaagc gccactacgt cacaatgcgg
    14041 tagaaatttt tgacgaagta aacaccggca aaaataccgg tagcggcgta ccgtgggtca
    14101 cctgggacat catccccgac aatgacgatg cggaaatcga agtttacatg gcaggcggcg
    14161 gctgcacgct acctggccgc tcgaaagtgt taatgccgtc agaaggctac gaaggcgtgg
    14221 tgaaattcgt cttcgaaaat atctccaccc tcgccgtaaa tgcctgtcca ccggtactgg
    14281 tgggcgtggg catcgccacc tcggtggaaa ccgccgccgt actctcgcgt aaagccattt
    14341 tgcgcccgat tggcagccgc catcccaatc caaaagcggc agaactggag ctacgcctgg
    14401 aagaaggact caaccgtctg gggattggtc cacaagggct gaccggcaac agttcagtga
    14461 tgggcgtaca tatcgaatct gccgcccgcc atccgtcaac catcggcgtt gctgtctcta
    14521 ccggctgctg ggcgcatcgt cgcggcacgc tgctggttca tgccgatctc acctttgaaa
    14581 atctgtctca cacccggagc gcgttatgaa aaagatcctg acaaccccga tcaaagctga
    14641 agatctgcaa gatattcgcg tcggcgatgt gatctacctg accggtacgc tggtgacctg
    14701 ccgcgacgtt tgtcaccgcc gtttgatcga actgaaacgt ccgatccctt acgatctcaa
    14761 cggcaaagcg attttccacg ctggccccat cgtgcgcaaa aacggcgaca aatgggagat
    14821 ggtctccgtc ggcccgacaa ccagtatgcg tatggaaagt tttgaacgtg aatttattga
    14881 gcagaccggc gtgaaactgg tggttggcaa aggtggtatg gggccgctga ccgaagaagg
    14941 ctgccagaaa ttcaaggcgc tacatgtgat tttcccggca ggctgcgcgg tgctggcggc
    15001 aacccaggtg gaagagattg aagaagtgca ctggacagag ctcggaatgc cggagtcact
    15061 gtgggtctgc cgggtcaaag agttcggccc gctgattgtc tctattgata cccacggcaa
    15121 caacctgata gccgaaaaca aaaagctgtt cgccgaacgc cgcgatccca tcgtggaaga
    15181 gatctgcgag cacgtccatt acatcaaata accctcccgg agaggctcac ccctctcctt
    15241 tttcgcaggc ataacacgat gaaaccttcc actgaatggt ggcgatatct tgcgccgctg
    15301 gcggtcatcg ccattattgc tctacttccg gttcccgcag ggctggagaa ccatacctgg
    15361 ctctactttg ccgtttttac tggcgtgatc gttggactga tcctcgaacc cgtgccgggt
    15421 gccgtggtgg cgatggtggg tatatccatc atcgccatac tctctccctg gctgctgttc
    15481 agcccggagc agctcgctca gccaggcttt aaattcactg caaaatccct ctcgtgggcc
    15541 gtttccggtt tttctaattc ggttatctgg ctgattttcg ccgcctttat gtttggcaca
    15601 ggctatgaaa aaaccgggct tggacgccgc attgcgctga ttctggtgaa aaagatggga
    15661 catcgcacgc tgtttctcgg ctatgcggtg atgttctccg agctgatcct cgcacctgta
    15721 acaccgtcca actcggcgcg tggtgcgggg attatctatc ccatcatccg taacctgcca
    15781 ccgctctatc aatcacaacc aaacgacagc agttcgcgca gcattggctc gtacatcatg
    15841 tggatgggga ttgttgccga ctgcgtgacc agcgccattt tcttgacggc gatggcacca
    15901 aacttgctgt taattggact gatgaaaagc gcatctcacg ccacgctgag ttggggcgac
    15961 tggttcctcg ggatgttgcc gctcagcatt ttactggttc tgctggttcc ctggctggct
    16021 tacgtgctgt acccgccggt actgaagtct ggcgatcagg tgccgcgctg ggcagagacg
    16081 gaactgcagg caatgggccc gctctgttcg cgtgaaaaac ggatgctggg gctgatggta
    16141 ggcgcgctgg tgctgtggat tttcggcggt gattatatcg atgccgcgat ggtcggttac
    16201 agcgtggtgg cactgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat
    16261 aaagcggcgt ggaacgtttt cttctggctg gcctcgctta tcaccctcgc caccggactc
    16321 aacaacaccg gttttattag ctggtttggc aaactgttag caggcagctt aagcggttat
    16381 tcgccaacgc tggtgatggt ggcgttgatt gtggtgtttt atctactgcg ctactttttc
    16441 gccagcgcca cggcgtatac ctccgctctc gcaccgatga tgattgccgc tgcgctggcg
    16501 atgccggaaa tcccgctgcc ggtgttctgc ctgatggttg gtgcggcaat tggtctgggg
    16561 agcattctta caccatacgc caccggcccc agtccgattt actacggcag tggttatctg
    16621 ccaacggcgg attactggcg actgggggcg atttttgggc tgatattcct cgtattgctg
    16681 gtgattaccg gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa
    16741 attgctctcc ggcacccaat agttttacgc agccggtaac tccgccagcg gccagcgcgg
    16801 acgcacgcta acgccgagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata
    16861 ggcgatcatc gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca cttcgccgcg
    16921 gcgttttttc atcatttcag ccagcttcgc ccgtaacgta cggttagcac tcacgccgcc
    16981 cgccatgacc agtcgcttaa agcccgtctg atccagcgcc cgcttgcact taatcatcag
    17041 cgtatcgacc accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt
    17101 gccgttgtca cgaatggtat ttgccgcgaa ggttttcagg ccggagaagc tgaaatccag
    17161 ccccggacgg tcggtcatcg gacgcgggaa gacaaagcgc ccggcagtac cctgagccgc
    17221 cattttcgac agtaacggcc cgccaggata atccagcccc agcagcttcg cggttttatc
    17281 aaacgcttcc ccggcggcat catcgataga ctcgccgagc agctcgtact gaccaatgcc
    17341 agtcacgctg attaactgcg tatgaccgcc ggaaacaagc agcgcaacaa acggaaattc
    17401 cggcgggtta tcttccagca tcggcgctaa cagatgccct tccatatggt gtacagggat
    17461 cgccggaacg tcccaggcaa acgccagaga acgccccacg gtcgcgccaa ccagtagcgc
    17521 gccgactaat ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaaacc
    17581 agactccttt agcgccgcct ggatcaacgg tacggtttta cgcacatgat cgcgggaggc
    17641 cagttcaggc acgacgccgc cgtagtcagc gtgcaatttc acctgactat acaattggtt
    17701 ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc
    17761 aatacccagt acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca
    17821 atgcgggttg atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc
    17881 cttataaaag tccctttcaa aaaaggccgc ggtgctttac aaagcagcag caattgcagt
    17941 aaaattccgc accattttga aataagctgg cgttgatgcc agcggcaaac cgaattaatc
    18001 aaaggtgaga ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt cgacgtagct
    18061 ctgcgtcgct tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt tcgtcgtcgt
    18121 gagttctatg aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc agtgaaacgt
    18181 cacgcgaaga aactggctcg cgaaaacgca cgccgcactc gtctgtacta attccccgag
    18241 agcgttgctc tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg aaaggaatgc
    18301 gcggcttatt ttcgtttatg aattgctaaa aatcggggcc tatggctgga cgaatcccac
    18361 gcgtattcat taatgatctg ctggcacgca ctgacatcgt cgatctgatc gatgcccgtg
    18421 tgaagctgaa aaagcagggc aagaatttcc acgcgtgttg tccattccac aacgagaaaa
    18481 ccccgtcctt caccgttaac ggtgagaaac agttttacca ctgctttgga tgtggcgcgc
    18541 acggcaacgc gatcgacttc ctgatgaact acgacaagct cgagttcgtc gaaacggtcg
    18601 aagagctggc agcaatgcac aatcttgaag tgccatttga agcaggcagc ggccccagcc
    18661 agatcgagcg ccatcagcgg caaacgcttt atcagttgat ggacggtctg aatacgtttt
    18721 accaacaatc tttacagcaa cctgttgcca cgtctgcgcg ccagtatctg gaaaaacgcg
    18781 gattaagcca cgaggttatc gctcgctttg cgattggttt tgcgcccccc ggctgggaca
    18841 acgtcctgaa gcggtttggc ggcaatccag aaaatcgcca gtcattgatt gatgcgggca
    18901 tgttggtcac taacgatcag ggacgcagtt acgatcgttt ccgcgagcgg gtgatgttcc
    18961 ccattcgcga taaacgcggt cgggtgattg gttttggcgg gcgtgtgctg ggcaacgata
    19021 cccccaaata cctgaactcg ccggaaacag acattttcca taaaggccgc cagctttacg
    19081 gtctttatga agcgcagcag gataacgctg aacccaatcg tctgcttgtg gtcgaaggct
    19141 atatggacgt ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg tcgttaggta
    19201 cgtcaaccac cgccgatcac atacaactgt tgttccgcgc gaccaacaat gtcatttgct
    19261 gttatgacgg cgaccgtgca ggccgcgatg ccgcctggcg agcgctggaa acggcgctgc
    19321 cttacatgac agacggccgt cagctacgct ttatgttttt gcctgatggc gaagaccctg
    19381 acacgctggt acgaaaagaa ggtaaagaag cgtttgaagc gcggatggag caggcgatgc
    19441 cactctccgc atttctgttt aacagcctga tgccgcaagt tgatctgagt acccctgacg
    19501 ggcgcgcacg tttgagtacg ctggcactgc cattgatatc gcaggtgccg ggcgaaacgc
    19561 tgcgaatata tcttcgtcag gaattaggca acaaattagg catacttgat gacagccagc
    19621 ttgaacgatt aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt ccgcagctaa
    19681 aacgcacgac catgcgtata cttatagggt tgctggtgca aaatccagaa ttagcgacgc
    19741 tagtcccgcc gcttgagaat ctggatgaaa ataagctccc tggacttggc ttattcagag
    19801 aactggtcaa cacttgtctc tcccagccag gtctgaccac cgggcaactt ttagagcact
    19861 atcgtggtac aaataatgct gccacccttg aaaaactgtc gatgtgggac gatatagcag
    19921 ataagaatat tgctgagcaa accttcaccg actcactcaa ccatatgttt gattcgctgc
    19981 ttgaactgcg ccaggaagag ttaatcgctc gtgagcgcac gcatggttta agcaacgaag
    20041 aacgcctgga gctctggaca ttaaaccagg agctggcgaa aaagtgattt aacggcttaa
    20101 gtgccgaaga gcgatcggga agcccccgac agccgcactg agaggcagcg gcaaatatat
    20161 aagtacgccc tcgtaattat cgttggcggt aaacaaccgt tggatttcag cgttaacggc
    20221 tgaaggacat cgggtcaatc gcccaacacc aacctcatga aataagtgtg gataccgtct
    20281 tatggagcaa aacccgcagt cacagctgaa acttcttgtc acccgtggta aggagcaagg
    20341 ctatctgacc tatgccgagg tcaatgacca tctgccggaa gatatcgtcg attcagatca
    20401 gatcgaagac atcatccaaa tgatcaacga catgggcatt caggtgatgg aagaagcacc
    20461 ggatgccgat gatctgatgc tggctgaaaa caccgcggac gaagatgctg ccgaagccgc
    20521 cgcgcaggtg ctttccagcg tggaatctga aatcgggcgc acgactgacc cggtacgcat
    20581 gtacatgcgt gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa ttgacatcgc
    20641 taagcgtatt gaagacggga tcaaccaggt tcaatgctcc gttgctgaat atccggaagc
    20701 gatcacctat ctgctggaac agtacgatcg tgttgaagca gaagaagcgc gtctgtccga
    20761 tctgatcacc ggctttgttg acccgaacgc agaagaagat ctggcaccta ccgccactca
    20821 cgtcggttct gagctttccc aggaagatct ggacgatgac gaagatgaag acgaagaaga
    20881 tggcgatgac gacagcgccg atgatgacaa cagcatcgac ccggaactgg ctcgcgaaaa
    20941 atttgcggaa ctgcgcgctc agtacgttgt aacgcgtgac accatcaaag cgaaaggtcg
    21001 cagtcacgct gctgctcagg aagagatcct gaaactgtct gaagtattca aacagttccg
    21061 cctggtgccg aagcagtttg actacctggt caacagcatg cgcgtcatga tggaccgcgt
    21121 tcgtacgcaa gaacgtctga tcatgaagct ctgcgttgag cagtgcaaaa tgccgaagaa
    21181 aaacttcatt accctgttta ccggcaacga aaccagcgat acctggttca acgcggcaat
    21241 tgcgatgaac aagccgtggt cggaaaaact gcacgatgtc tctgaagaag tgcatcgcgc
    21301 cctgcagaaa ctgcagcaga ttgaagaaga aaccggcctg accatcgagc aggttaaaga
    21361 tatcaaccgt cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga aagagatggt
    21421 tgaagcgaac ttacgtctgg ttatttctat cgccaagaaa tacaccaacc gtggcttgca
    21481 gttccttgac ctgattcagg aaggcaacat cggtctgatg aaagcggttg ataaattcga
    21541 atatcgccgt ggttacaagt tctccaccta cgcaacctgg tggatccgtc aggcgatcac
    21601 ccgctctatc gcggatcagg cgcgcaccat ccgtattccg gtgcatatga ttgagactat
    21661 caacaaactc aaccgtattt ctcgccagat gctgcaagag atgggccgtg aaccgacgcc
    21721 ggaagaactg gctgaacgta tgctgatgcc ggaagacaag atccgcaaag tgctgaagat
    21781 cgccaaagag ccaatctcca tggaaacgcc gatcggtgat gatgaagatt cgcatctggg
    21841 ggatttcatc gaggatacca ccctcgagct gccgctggat tctgcgacca ccgaaagcct
    21901 gcgtgcggca acgcacgacg tgctggctgg cctgaccgcg cgtgaagcga aagttctgcg
    21961 tatgcgtttc ggtatcgata tgaacaccga ccacacgctg gaagaagtgg gtaaacagtt
    22021 cgacgttacc cgcgaacgta tccgtcagat cgaagcgaag gcgctgcgca aactgcgtca
    22081 cccgagccgt tctgaagtgc tgcgtagctt cctggacgat taatcggtag gccggatcag
    22141 gtgttacgcc gcacccggca cctgtccctt tgcacaaacg ccaccttttc ggtggcgttt
    22201 tttatcgccc acgcaccacc agcgcctggt ccagctcgcg atacgcttca accagtttct
    22261 ccagtgaaac gcgacttaaa ccgctgggat ttggcagcac ccaaatctgt gtcgaaccaa
    22321 tggtgagcgt ttgtttcccc cactgtgcac cgcgctggct gaatccctgt tcatatgctt
    22381 gtttgcccag aatcgccaac gcctgcggct gataatcttc aattttttca atcagcttac
    22441 gcccgcctgc gtgtagctcc tgctttgaaa cttcattggc ttgcaccgtt ggacggtcta
    22501 ccagtttggt gacgccacaa cgataatcca gcagatgctg tgcctcctgc ggcttcaact
    22561 gacggtcggt aaacccggcc tgatatatca ccttccagaa gcgatttgcc ggatgagcaa
    22621 agggaaaacc agtcccggcg gatgaaagcc cagggttgat accgcaaaac acgacccgta
    22681 accctggagc caaaatatcc tcaaccatat ttactccttt ctaacatcac caggaaaagt
    22741 ataacggatt gaaaatacat tgtttataaa aacagcaggc gcgcggtaat ggctggattg
    22801 cgacacggag ttactttata atccgctacc atggcccctt agctcagtgg ttagagcagg
    22861 cgactcataa tcgcttggtc gctggttcaa gtccagcagg ggccaccaga tatagcaaag
    22921 gctgacgaga aatcgtcagc ctttttcttt ttatatatca gttactttgc gtgccagtaa
    22981 gccgctgcac gtacccgctg tgggtcatac tgttccgctt caaagcggcg gcttaaattc
    23041 ttaacgactt taccttcgcc ggttatccag atgaagtaat catcggcagg gatttgcatc
    23101 tgcgccagac gcgcatctac cgcctgctca tcatgtgcca gccattcgat attaaaacca
    23161 tcaaggtgcg cgagataatc ctgacaggcg ttatcccgca cgctaaccag cgcactaact
    23221 tgcggtttaa cggcaagttt gctcaacgtt tccaggcggc ggcgcaatgc gggcattccg
    23281 gattcatcgc agacatacag ctgatacgcg taatcttccg gcaccaccag cgaaccgcgc
    23341 ggacctgcca ccgtaagttt atcgcccggt tgcgcctgca tcgcccagcc gctagcgacc
    23401 ccaccgtcgt gaataaagaa atcgatcgcc agttcatggc gtagttcgtc atacagcggc
    23461 gtatagtcac gcgacggtgg gcgtggtcct tccggccaga cgatgccctc ttccgttacc
    23521 gttggcggca caaagtgagc gtcaggttga ggaaagaaga gtttgcagtg atcgtcaaag
    23581 ccacgcgatg taaaaccgtc cagcgcctcg ccgccgagga caatgcgctg aaaaccggcg
    23641 ctgatgcgct caacgcgtaa cacagtcagt tcacggaagc gcagatcatt gcgaacgcgc
    23701 tgcgggtagc ggggggtgtt attcattgtt atcgccttcg tgatggtaat tagatatatc
    23761 taaataattt tcgcaaatga taatgattgt taatcatgat aaatgcaagc gatttgtaga
    23821 actgatatgt ctatagtctg acaagacgaa ccgcctcttc tcaggcatca ttactcaacg
    23881 ccggatgcgg cgtgaacgcc ttatccggcc tacgtgtgag atgagtccac atttaaaaat
    23941 catttttatt cttgcaagag tgctcatatc agatataaat tagatatatc taattaagca
    24001 aaaggaggct gatatgagcc atcatcacga agggtgttgt aaacatgaag gccagccacg
    24061 ccatgagggc tgctgcaaag gtgagaagtc agaacacgag cactgcggac acggtcacca
    24121 gcatgaacac ggtcaatgct gcggtggtcg ccacggtcgc ggcggcggtc gtcggcaacg
    24181 tttctttggt cacggtgaat tgcgtctggt gattctggat attctctcgc gcgatgacag
    24241 ccacggttac gaattgatta aagcgattga gaacctaacc caggggaatt acaccccaag
    24301 cccgggcgtc atctacccaa cgctggattt tctgcaggag cagtcgctga ttaccatccg
    24361 cgaagaggaa ggtggtaaga agcagattgc gctgaccgaa caaggcgcgc agtggctgga
    24421 agaaaaccgc gaacaggtgg agatgattga agaacgcatc aaagcgcgtt gcgttggcgc
    24481 ggcgctgcgc cagaacccgc aaatgaagcg ggcgctggat aattttaaag cggtgctgga
    24541 tttacgcgtc aaccagagcg atatcagtga tgcacaaata aaaaagatca ttgcggtgat
    24601 cgaccgcgcc gcttttgata ttacgcaact ggattaatcg ccgcatccgc cagtggcgcg
    24661 gtgcaattgc cggatgcgac gcttgacgcg ccttatccgg cctacacccg ctacacaccc
    24721 cgcaggcctg ataagatgcg ccagcatcgc atcaggcatt gtgctccaac cgccggatcc
    24781 ggcataccga ttaatgcagc accgtcaccg cgtcttccag tcggctggcg cggtgtttca
    24841 ccatcgccga cacctgcgca ctctcttcca ccagctcggc atttttctgg gtgatcaggt
    24901 taagctcatc cactgcacgg gtcaggctgg aaagcccatc ggcctgttcc agcgttgaat
    24961 ggctaatctg ggcgatcaac tgggtgacgt ttttcacctg tgccacaata tcttccatcg
    25021 tccgtccggc ggcgtgtacc tgctgcgaac cggattgcac cttatcagca ctggcatcaa
    25081 tcagcttgcg aatatcgttg gcagcattag cgctgcggct ggctaaatga cgcacttccc
    25141 ctgccaccac tgcaaaacct ttgccctgtt cgccggcacg cgccgcttcc accgccgcat
    25201 tcagggccag aatattggtc tgaaacgcaa tatcgttaat cagcgaagta atggtgccaa
    25261 tgcgctgggt actgtcggcg atatcgtcca ttgtcttgat caccgtggtc atcgcctccc
    25321 caccctgcac tgccgcatta ctggcggtga tagaaagttt atcggcagcc gacgccgtgg
    25381 cagagttctg tttcaccgac gccgccattt ggttcatggt cgccaccgtt tgctgaacgt
    25441 tatcaactgt ctgctgggta tgttcgttca gttcatcggt gcctttcgcc agcgtctcac
    25501 tgccatttct gacactggac acctggcttg agacatcgtt aattagccaa cggcacatca
    25561 ggccaagctg ccctaccgcg cgtaatgtca gccccagctc atcgctgcga ttcaggtgct
    25621 caacactatt acgctctccg gtcgccacct tcagtgcctg acgggcgaca ttttctatcg
    25681 ggcgcacaat ctgccattca aaacaagcgc ttgccaacaa taccactaac acacagagga
    25741 aatacgtcac caccggggca gcaacaaacc aaagcatggc cgccaacaag ataaacatca
    25801 gggtcatcac tccacgcgcc cgccagcgaa gcggtaatga aggcagttta cccagccagc
    25861 ctttacgcac caccaggcct ttatgaatac gcttactggt acgtccggcg ttcagcgctt
    25921 tgtacagcgg ctccaccgcc gcaatctctt catccgtcgc ccgggtacga atcgacatat
    25981 agccactgat ttttccctcg cgcaccatcg gtaccgcatt ggcccgcacc caataatggt
    26041 cgccattttt gcggcgattt ttcacgatgc cgctccaggg ctcccctttt ttcagggtga
    26101 accacatatc cgcaaacgcc gcttttggca tatccgggtg acgcaccatg ttgtgcggct
    26161 gcccttgtaa ctcttgcaag gtaaagccgc tcacctgcac aaaagtgtca ttagcatgag
    26221 tgatatagct ttgcagatcg gtagtggaca tcagagtggt atcgtccgcc agcggggtat
    26281 tttgctgggt gacatacgga tgagaagaca tgttcgcgtc ctgtgcaggt tatatggttg
    26341 ttaacttctt gtcagagttt atgtcggccc cgctgcggtt atctttaacc gattaatttg
    26401 atttagatcg caatttgcga ttaaagcaca aatctaattc tttgatttaa aatactttca
    26461 ctctgttact atacgaaaac gttaattatc ttgcccaaaa atcaggcaat tattgccctg
    26521 aaaacgtgca tttgcgcagc aatcatcaaa tccatacccg acaaaaaccg tgcaaaataa
    26581 caacaaatgt taacagatag cattaaatat tgcacaaatg ataaccgaat ttgtgtttat
    26641 cccgattttc gcgatcgcag ccggagtggc gcaatccctg caatacttaa atcggtatca
    26701 tgtgatacgc gagcctccgg agcatatttt gaacaggtta ccttcgagcg catcggcttt
    26761 agcgtgcagc gcccacgccc tgaatctcat tgaaaagcga acgctggatc atgaggagat
    26821 gaaagcactt aaccgagagg tgattgaata cttcaaagag catgtcaatc cggggttttt
    26881 agagtatcgc aaatctgtta ccgccggcgg ggattacgga gccgtagagt ggcaagcggg
    26941 aagtttaaat acgcttgtcg acacccaggg acaggagttt atcgactgcc tgggaggttt
    27001 tggaattttc aacgtggggc accgtaatcc agttgtggtt tccgccgtac agaatcaact
    27061 tgcgaaacaa ccgctgcaca gccaggagct gctcgatccg ttacgggcga tgttggcgaa
    27121 aacccttgct gcgctaacgc ccggtaaact gaaatacagc ttcttctgta atagcggcac
    27181 cgagtccgtt gaagcagcgc tgaagctggc gaaagcttac cagtcaccgc gcggcaagtt
    27241 tacttttatt gccaccagcg gcgcgttcca cggtaaatca cttggcgcgc tgtcggccac
    27301 ggcgaaatcg accttccgca aaccgtttat gccactgctg ccgggcttcc gtcatgtgcc
    27361 gtttggcaat atcgaagcca tgcgcacggc tcttaacgag tgcaaaaaaa ccggtgatga
    27421 tgtggctgcg gtgatcctcg aaccgattca gggtgaaggt ggcgtaattc tgccgccgcc
    27481 gggctatctc accgccgtac gtaagctatg cgatgagttc ggcgcactga tgatcctcga
    27541 tgaagtacaa acgggcatgg ggcgcacggg caagatgttc gcctgcgagc atgagaacgt
    27601 acagccggat atcctctgcc ttgccaaagc gctcggcggc ggcgtgatgc cgattggcgc
    27661 gaccatcgcc actgaagagg tgttttcagt tctgttcgac aacccattcc tgcataccac
    27721 cacctttggc ggcaacccgc tggcctgtgc ggcggcgctg gcgaccatca atgtgttgct
    27781 ggagcagaac ttaccggctc aggctgagca aaaaggcgat atgttgctgg acggtttccg
    27841 tcaactggcg cgggaatatc ccgatctggt acaggaagcg cgtggtaaag ggatgttgat
    27901 ggcaattgag tttgttgata acgaaatcgg ctataacttt gccagcgaga tgttccgcca
    27961 gcgcgtactg gtggccggaa cgctcaataa cgccaaaacg atccgcattg aaccgccact
    28021 gacactgacc attgaacagt gtgaactggt gatcaaagcg gcgcgtaagg cgctggcggc
    28081 catgcgagta agtgtcgaag aagcgtaata tcaaatcgga tggcgatacg acgtcgccat
    28141 ccgatctttt tatacgacgc gtacgcccgc aggcatcatc cgctccggcg ttaacaacac
    28201 gctttcactg ccgtcatcgg tttccgcaca caacagcatg cattccgacg tttcaccacg
    28261 cattttcgct ttttgcagat tgcataagac caccaccgtt ttccccatca gctcttcttc
    28321 gctgtagtac ggcaccaggc tggtcacggt ttgcagtgtt ttttccccca catcaacctg
    28381 tacgatgtac agcttgtcgg cgttttcatg gcgtttcact tccacaatct ttccgacgcg
    28441 catttccaga cgtgcaaaat cagcgtaagc cacggtttcc attgtttcct cccttcgggt
    28501 aaaattttac taaactatag caaaagtttt tctcaatcct gtaggctaaa aatggagaat
    28561 gcaggcgtga tcacattcgt gagccgctgt gttaccgtta cagcgtcaaa gaaacgcgct
    28621 ttatttactg aaaacaggtg acccgataag cacttcctct acaatggaag cgcacatcag
    28681 ggaaagtaaa aaaggtaaac atggcaacac taaaagacat cgcaatcgaa gctggcgtat
    28741 ccctggcgac agtatccagg gtcttaaatg acgatccgac attgaatgtg aaagaagaga
    28801 cgaaacatcg cattctcgag atcgccgaaa agctggagta caagaccagt agtgcccgta
    28861 aactccagac aggtgcagtc aaccaacacc atattctggc tatctacagc taccagcagg
    28921 agctggagat caacgatcct tactatctgg cgatccgcca cggcattgaa actcagtgcg
    28981 aaaagctggc gatcgaactg accaactgtt atgaacacaa cggattacca gacattaaaa
    29041 acgtcaccgg tattttaatt gtcggcaaac ccacgcccgc cctgcgcgcc gctgccagcg
    29101 cgttgaccga caatatctgt tttatcgact ttcacgaacc cggcagcggt tacgatgcgg
    29161 tggatatcga tctggcacgc atcagtaaag aaatcatcga cttctatatc aaccagggcg
    29221 ttaatcgtat tggttttatt ggcggtgaag atgagcctgg caaggcggat attcgtgagg
    29281 tcgcctttgc ggaatatggc cgactgaaac aagtggtacg cgaagaggat atctggcgcg
    29341 gcggtttttc cagttcgtcg ggttatgaac tggcaaaaca aatgctggcg cgggaagact
    29401 atccgaaggc actgtttgtt gcttccgatt ccattgctat cggcgtactg cgggcaattc
    29461 atgaacgtgg cctgaacatc ccacaggata tttcgcttat cagcgttaac gatatcccca
    29521 ccgcgcgatt tacctttccg ccgctctcca ccgtgcgcat ccattccgag atgatgggaa
    29581 gtcagggcgt taacctggtg tatgaaaaag cccgcgatgg tcgcgcgctg ccgctgctag
    29641 tcttcgttcc cagcaaatta aaactgcgcg gcacgacccg ttaaatcccc ttacacactg
    29701 tccggcaatc atttttgccg gacagtgctg ccgtttattt tcgtgatcca gttaaagtaa
    29761 atgcatttac ctgctacttt ttagtaaaaa ttttactaaa ctccccagca attacacaaa
    29821 ctaccatcac catgaatggt tccgatttct ctctaccggg aggccctatg aatcgctggg
    29881 aaaacattca gctcacccac gaaaaccgac ttgcgccgcg tgcgtacttt ttttcatatg
    29941 attctgttgc gcaagcgcgt acctttaccc gcgaaaccag cagcctgttt ctgcccttaa
    30001 gcggtcagtg gaatttccat ttttttgacc acccgctgca agtacctgaa gccttcacct
    30061 ctgagttaat ggctgactgg gggcatatta ccgtccccgc catgtggcaa atggaaggtc
    30121 acggcaaact gcaatatacc gacgaaggtt ttccgttccc catcgatgtg ccgtttgtcc
    30181 ccagcgataa cccaaccggt gcctatcaac gtattttcac cctcagcgac ggctggcagg
    30241 gtaaacagac gctgattaaa tttgacggcg tcgaaaccta ttttgaagtc tacgttaacg
    30301 gtcagtatgt gggtttcagc aagggcagtc gcctgaccgc agagtttgac atcagcgcga
    30361 tggttaaaac cggcgacaac ctgttgtgtg tgcgcgtgat gcagtgggcg gactctacct
    30421 acgtggaaga ccaagatatg tggtggtcag cggggatctt ccgcgatgtt tatctgatcg
    30481 gaaaacacct aacgcatatt aacgatttca ctgtgcgtac cgactttgac gaagcctatt
    30541 gcgatgccac gctttcctgc gaagtggtgc tggaaaatct cgcagcctcc cctgtcgtaa
    30601 cgacgctgga atataccctg tttgatggcg aacgcgtggt gcacagcagc gccattgatc
    30661 atttggcaat tgaaaaactg accagcgcca gctttgcttt tactgtcgaa cagccccagc
    30721 aatggtcagc agaatcccct tatctttacc atctggtcat gacgctaaaa gacgccgacg
    30781 gcaacgttct ggaagtggtg ccacaacgcg ttggcttccg tgatatcaaa gtgcgcgacg
    30841 gtctgttctg gatcaataac cgttatgtga tgctgcacgg cgtcaaccgt cacgacaacg
    30901 atcatcgcaa aggccgcgcc gttggaatgg atcgcgtcga gaaagatctc cagttgatga
    30961 agcagcataa catcaactcc gtgcgtaccg ctcactaccc gaacgatccg cgtttttacg
    31021 aactgtgtga tatctacggc ctgtttgtga tggcggaaac cgacgtcgaa tcgcacggct
    31081 ttgctaatgt cggcgatatc agccgtatta ccgacgatcc gcagtgggaa aaagtctacg
    31141 tcgagcgcat tgttcgccat atccacgcgc agaaaaacca tccgtcgatc atcatctggt
    31201 cgctgggcaa tgaatccggc tatggctgta acatccgcgc gatgtaccac gcagcgaagg
    31261 cgctggatga cacgcgactg gtgcattacg aagaagatcg cgatgctgaa gtggtcgata
    31321 ttatttccac catgtacacc cgcgtgccgc tgatgaatga gtttggtgaa tacccgcatc
    31381 cgaagccgcg catcatctgt gaatatgctc atgcgatggg gaacggaccg ggcgggctga
    31441 cggagtacca gaacgtcttc tataagcacg attgtattca gggacattat gtttgggagt
    31501 ggtgcgacca cggaatccag gcgcaggatg acaacggcaa tgtctggtat aaattcggcg
    31561 gcgactacgg cgactatccc aacaactata acttctgtct tgatggtttg atctattccg
    31621 atcagacgcc gggaccaggc ctgaaagagt acaaacaggt tatcgcgccg gtaaaaatcc
    31681 acgcgctgga tctgactcgc ggcgagctga aagtcgaaaa taaactgtgg tttaccacgc
    31741 ttgatgacta caccctgcac gcagaggtgc gtgccgaagg tgaaacgctc gcaacgcagc
    31801 agattaaact gcgcgacgtt gcgccgaaca gcgaagcccc cttgcagatc acgctgccgc
    31861 agctggacgc ccgcgaagcg ttcctcaaca ttacggtgac caaagattcc cgcacccgct
    31921 acagcgaagc cgggcattct atcgccactt atcagttccc gctgaaggaa aacaccgcgc
    31981 agccagtgcc tttcgcacca aataatgcgc gtccgctgac gctggaagac gatcgtttga
    32041 gctgcaccgt tcgcggctac aacttcgcga tcaccttctc aaaaatgagt ggcaaaccga
    32101 catcctggca ggtaaatggc gagtcgctgc tgacccgcga gccaaagatc aacttcttca
    32161 agccgatgat cgacaaccac aagcaggagt acgaagggct gtggcaaccg aatcatttgc
    32221 agatcatgca ggaacatctg cgcgactttg ccgtagaaca gagcgatggt gaagtgttga
    32281 tcatcagccg cacggttata gcaccgccgg tgtttgactt cgggatgcgc tgcacctaca
    32341 tctggcgcat cgctgcagat ggccaggtta acgtggcgct ttccggcgag cgttacggcg
    32401 actatccgca catcattccg tgcatcggtt tcaccatggg gattaacggc gaatacgatc
    32461 aggtggcgta ttacggtcgt ggaccgggcg aaaactacgc cgacagccag caggctaaca
    32521 tcatcgatat ctggcgcagc accgtcgatg ccatgttcga gaactatccc ttcccgcaga
    32581 acaacggcaa ccgtcagcat gtccgctgga cggcactgac taaccgccac ggcaacggtc
    32641 tgctggtggt tccgcagcgc ccaattaact tcagcgcctg gcactatacc caggaaaaca
    32701 tccacgcttc ccagcactgt aacgagctgc agcgcagtga tgacatcacc ctgaatctcg
    32761 accaccagct gcttggcctc ggctccaact cctggggcag cgaggtgctg gactcctggc
    32821 gagtctggtt ccgtgacttc agctacggct ttacgttgct gccggtttct ggcggagaag
    32881 ctaccgcgca aagcctggcg tcgtatgagt tcggcgcagg gttcttttcc acgaatttgc
    32941 acagcgagaa taagcaatga ggatcatcga taacttagaa cagttccgcc agatttacgc
    33001 ctctggcaag aagtggcaac gctgcgttga agcgattgaa aatatcgaca acattcagcc
    33061 tggcgtcgcc cactccatcg gtgactcatt gacttaccgc gtggagacag actccgcgac
    33121 cgatgcgcta tttaccgggc atcgacgcta ttttgaagtg cattactacc tgcaagggca
    33181 gcaaaaaatt gaatatgcgc cgaaagagac attacaggta gtggaatatt atcgtgacga
    33241 aactgaccgc gaatatttaa aaggctgcgg agaaaccgta gaggtccacg aagggcaaat
    33301 cgttatttgc gatatccatg aagcatatcg gtttatctgc aataatgcgg tcaaaaaagt
    33361 ggttctcaaa gtcaccatcg aagatggtta tttccataac aaataacaac tacggcggca
    33421 aaaggagttt gccgccaccg ctaccctact cattttcgga gatgtgttat gtctgatacc
    33481 aaacgtaata caatcggcaa attcggcttg ctctcgctga cttttgccgc cgtttacagc
    33541 tttaacaacg ttatcaataa taatattgag cttggactgg cctcggcacc gatgtttttc
    33601 ctcgcgacga ttttttattt tattcccttc tgtctgatca tcgcagaatt tgtttcgtta
    33661 aataaaaact cagaagccgg tgtctacgcg tgggtaaaaa gttcgctggg cggacgttgg
    33721 gcatttatta ctgcctacac ttactggttc gtaaatctgt tctttttcac ctcgctgttg
    33781 ccgcgcgtta ttgcttatgc ttcgtatgcc ttcctcggct acgaatatat tatgacgccg
    33841 gttgccacca ccgttatcag tatggtgctg ttcgccttct ccacctgggt ttccaccaac
    33901 ggggcgaaaa tgctggggcc aattacctcc gtcacttcaa cgctgatgct gctgttaacg
    33961 ctctcctaca ttttactggc aggtacggca ctggttggcg gcgtacagcc tgccgatccg
    34021 atcaccgttg acgcgatgat cccgaacttc aattgggcgt tcctcggcgt taccacctgg
    34081 atctttatgg ccgcaggtgg cgcggagtcc gtcgctgtgt acgttaacga cgtcaaaggc
    34141 ggttcgaaat cgttcgttaa agtgatcatc ctcgccggga tttttatcgg tgtgctgtat
    34201 tccgtctcct cggtgctgat taacgtcttc gtcagcagca aagagttgaa atttaccggc
    34261 ggatcggtgc aggtattcca cggcatggcg gcgtattttg gtctgccgga agcgttgatg
    34321 aatcgctttg tcggtctggt gtcctttacc gcgatgttcg gttccctgct gatgtggacc
    34381 gcaacgccgg tgaaaatttt cttctccgaa atcccggaag gcatctttgg taagaaaacc
    34441 gtcgaactga acgaaaacgg cgttccggcg cgcgcagcgt ggatccagtt cctgatcgtc
    34501 atcccgctga tgattatccc gatgctcggt tccaataccg tgcaggatct gatgaatact
    34561 attattaata tgactgccgc agcgtccatg cttccgccgt tattcatcat gctggcttac
    34621 ctgaatttac gtgccaaatt agatcacctg ccacgcgatt tccgtatggg ctcccgccgc
    34681 accggtatta tcgttgtttc aatgctgatt gcgatatttg ccgtagggtt tgtcgcttcg
    34741 acattcccga ctggcgcgaa tattctgacc atcatttttt ataacgtcgg cggtattgtt
    34801 atcttcctcg gttttgcgtg gtggaaatac agtaaatata taaagggatt aacggctgaa
    34861 gaacgccata ttgaagcgac gccagccagc aatgttgatt aagcagaata ataaaaaatg
    34921 agcaggcatt ttccctcttc ctggatgagg agagggaaaa tagtttctgc ctttatattt
    34981 ttataattac aacgataaaa ggctgtactt tttctttagc tcatggatta acacaatgaa
    35041 attaatcact gcaccatgca gagcattact tgctctgccg ttttgctacg ccttttctgc
    35101 ggcaggagaa gaagcacgtc cggcagaaca tgacgacaca aaaacacccg caattacctc
    35161 gacatcttct ccttcatttc gtttttacgg cgaattaggg gttggtggat atatggattt
    35221 agagggtgag aataaacata aatacagcga cggtacctat attgaaggtg gcctggagat
    35281 gaagtacggc tcctggttcg gcctgattta cggcgaaggc tggaccgtgc aggccgacca
    35341 cgacggcaat gcctgggtgc cagaccatag ctggggtggt ttcgagggcg gaattaaccg
    35401 tttctatggc ggttatcgta ccaatgatgg caccgaaatc atgctcagtc tgcgtcagga
    35461 ttcctcactg gatgacctgc aatggtgggg cgacttcacc cccgatctgg gctacgtcat
    35521 tcccaatacc cgcgacatta tgaccgcgct gaaggtgcag aacttaagcg gcaactttcg
    35581 ttatagcgtc accgccacac ctgccggaca tcatgacgaa agcaaagcct ggctacattt
    35641 tggcaagtac gatcgctatg acgacaaata cacctatccg gcaatgatga acggttacat
    35701 ccagtatgac cttgccgaag gcatcacctg gatgaacggt ctggaaatta ccgacggcac
    35761 cggacaactc tatctcaccg gcctgcttac ccctaacttc gccgcccgcg cctggcacca
    35821 taccggacgc gccgacgggc tggacgtacc gggaagtgaa agtggaatga tggtgagcgc
    35881 catgtatgaa gcattaaagg gcgtttatct ctccaccgct tacacctacg ccaaacatcg
    35941 ccctgaccac gctgacgatg aaaccacctc tttcatgcag tttggtatct ggtacgaata
    36001 tggcggcgga cgtttcgcaa cggcttttga tagccgcttc tacatgaaaa atgcctctaa
    36061 cgatcccagc gaccaaatct tcctgatgca atatttctac tggtaataag gactgtaatt
    36121 tcatgaaaat caaaactatt ttaacgccag taacctgcgc tctgctgata agtttttccg
    36181 cccatgctgc taacgccgac aattataaaa acgtgattaa ccgtactggc gcgccgcagt
    36241 acatgaagga ttacgattac gacgatcacc agcgttttaa tccgtttttc gatctcggag
    36301 cctggcatgg tcatctgttg ccagacggcc ctaacaccat gggcggtttt ccaggcgttg
    36361 cgctgctgac ggaagagtac atcaacttta tggccagcaa tttcgaccga ctgaccgtct
    36421 ggcaggacgg caagaaagtc gacttcacgc tggaggcata cagtattccc ggtgcgctgg
    36481 tgcaaaaact gacagcaaaa gatgtgcagg tcgaaatgac tctgcgcttc gccacgccgc
    36541 gcacgtcact actggaaacc aaaatcacca gcaataaacc gctggatctg gtgtgggacg
    36601 gcgaactgct ggaaaaactg gaagcgaaag aagggaaacc gctttccgat aaaaccattg
    36661 ctggcgagta ccctgactat cagcgcaaaa tcagcgccac ccgtgatggc ctgaaagtca
    36721 cctttggcaa agtgcgcgcc acctgggatc tgctgacctc cggcgaatcg gaatatcagg
    36781 tgcataaatc cctgccggtg cagactgaaa tcaacggcaa tcgctttacc agtaaggcac
    36841 atatcaacgg ttcgacgacg ctctatacca cctattccca tctgctgacc tctcaggaag
    36901 ttagcaaaga gcaaatgcag atccgcgata ttctggcgcg tccggcgttt tatctcaccg
    36961 cctcgcagca acgctgggaa gagtatctga agaaagggtt aaccaatccg gatgcgacgc
    37021 cggaacagac gcgcgtcgcg gtgaaagcga tcgaaacgct caacggtaac tggcgttcgc
    37081 ctggcggggc ggtgaaattt aacaccgtca caccgtcggt gaccgggcgc tggttctccg
    37141 gcaatcagac ctggccgtgg gatacctgga agcaggcgtt tgcgatggcg catttcaatc
    37201 cggacatcgc caaagagaat atccgcgcgg tcttctcctg gcagatccag ccaggcgacc
    37261 gcgtgcgtcc gcaggatgtg ggctttgtcc ccgacctgat agcgtggaac cttagccccg
    37321 agcgtggtgg cgatggcggc aactggaacg aacgtaatac caaacccagc cttgccgcct
    37381 ggtcggtgat ggaagtgtat aacgtcaccc aagataaaac ctgggtggca gagatgtacc
    37441 cgaaactggt ggcctatcac gactggtggt tacgtaaccg cgatcacaac ggcaacggcg
    37501 tgccggaata tggcgcgacc cgcgacaaag cccacaacac tgagagcggc gagatgctgt
    37561 ttacggtgaa aaaaggcgat aaagaagaga cgcagtctgg gctgaacaac tacgcccgcg
    37621 tggtggagaa aggccagtac gacagtctgg aaattccggc acaggttgct gcatcgtggg
    37681 aatcggggcg tgatgatgcc gccgtctttg ggtttatcga caaagaacag ttggataaat
    37741 atgtcgctaa tggcggcaaa cgtagcgact ggacggtgaa attcgccgaa aaccgcagtc
    37801 aggacggaac gttgctgggc tactcgctat tgcaggagtc ggtggatcag gccagctata
    37861 tgtacagcga taaccattat ctggcggaga tggcaaccat cctcggtaag ccagaagagg
    37921 ccaaacgcta tcgccagttg gcacagcagc tcgcggacta catcaacacc tgtatgttcg
    37981 acccgactac ccagttctac tatgacgtgc gtattgaaga taaaccgctg gcgaacggct
    38041 gcgcgggcaa accgattgtt gagcgcggta aagggccgga aggctggtcg ccgctgttta
    38101 acggtgcggc aacgcaggcc aacgccgacg cggtggtgaa ggtgatgctc gatcctaaag
    38161 agttcaacac ctttgtcccg ctgggaacgg cggcgttaac caacccggct tttggcgcgg
    38221 atatctactg gcgcgggcgc gtatgggtgg atcagttctg gtttggtctg aaagggatgg
    38281 agcgttacgg ttatcgcgat gatgccctga aactggcgga tacgttcttc cggcacgcca
    38341 aaggattaac cgccgatggc ccaattcagg agaattacaa cccgctgact ggcgcacagc
    38401 aaggcgcacc aaatttctcc tggagtgccg cgcatttgta tatgttgtat aacgattttt
    38461 tccgtaagca ataatttgca actgctggcg gatgcggcgt aaacgcctta tccgccctac
    38521 atgtgcaatc ctcctggccg gataaaacgc ggcaagcgtt gcatccggca actgcaccgc
    38581 gccactggcg gatgcggcgt gaacgcctta tccgccctac atgtgcgttc ccgtaggtcg
    38641 gataagacgc gacaagcgtc gcatccggca
//
LOCUS       DAOFQD010000032        38208 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.032, whole genome shotgun sequence.
ACCESSION   DAOFQD010000032 DAOFQD010000000
VERSION     DAOFQD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.032"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(142..447)
                     /locus_tag="QBF46_001713"
     CDS             complement(142..447)
                     /locus_tag="QBF46_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDQ6673904.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(555..631)
                     /locus_tag="QBF46_001714"
     tRNA            complement(555..631)
                     /locus_tag="QBF46_001714"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(595..597),aa:Val,seq:gac)
     gene            complement(636..712)
                     /locus_tag="QBF46_001715"
     tRNA            complement(636..712)
                     /locus_tag="QBF46_001715"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(676..678),aa:Val,seq:gac)
     gene            1020..2276
                     /locus_tag="QBF46_001716"
     CDS             1020..2276
                     /locus_tag="QBF46_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDQ6673905.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(2317..3690)
                     /gene="mdtK"
                     /locus_tag="QBF46_001717"
     CDS             complement(2317..3690)
                     /gene="mdtK"
                     /locus_tag="QBF46_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDQ6673906.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            3905..4546
                     /gene="ribE"
                     /locus_tag="QBF46_001718"
     CDS             3905..4546
                     /gene="ribE"
                     /locus_tag="QBF46_001718"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDQ6673907.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(4586..5734)
                     /gene="cfa"
                     /locus_tag="QBF46_001719"
     CDS             complement(4586..5734)
                     /gene="cfa"
                     /locus_tag="QBF46_001719"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDQ6673908.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(6025..7236)
                     /locus_tag="QBF46_001720"
     CDS             complement(6025..7236)
                     /locus_tag="QBF46_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDQ6673909.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            7349..8281
                     /locus_tag="QBF46_001721"
     CDS             7349..8281
                     /locus_tag="QBF46_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6673910.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(8278..9303)
                     /gene="purR"
                     /locus_tag="QBF46_001722"
     CDS             complement(8278..9303)
                     /gene="purR"
                     /locus_tag="QBF46_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDQ6673911.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            9602..9691
                     /gene="ynhF"
                     /locus_tag="QBF46_001723"
     CDS             9602..9691
                     /gene="ynhF"
                     /locus_tag="QBF46_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDQ6673912.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            9857..11026
                     /locus_tag="QBF46_001724"
     CDS             9857..11026
                     /locus_tag="QBF46_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6673913.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(11261..11842)
                     /gene="sodB"
                     /locus_tag="QBF46_001725"
     CDS             complement(11261..11842)
                     /gene="sodB"
                     /locus_tag="QBF46_001725"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDQ6673914.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGELADAITASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(11970..12785)
                     /gene="mepH"
                     /locus_tag="QBF46_001726"
     CDS             complement(11970..12785)
                     /gene="mepH"
                     /locus_tag="QBF46_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDQ6673915.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            13119..13466
                     /gene="grxD"
                     /locus_tag="QBF46_001727"
     CDS             13119..13466
                     /gene="grxD"
                     /locus_tag="QBF46_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDQ6673916.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(13517..18133)
                     /locus_tag="QBF46_001728"
     CDS             complement(13517..18133)
                     /locus_tag="QBF46_001728"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDQ6673917.1"
                     /translation="MADNPDPSSLLPDVFSPATREWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNCGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALNT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(18226..18873)
                     /gene="rnt"
                     /locus_tag="QBF46_001729"
     CDS             complement(18226..18873)
                     /gene="rnt"
                     /locus_tag="QBF46_001729"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDQ6673918.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPTAEEV"
     gene            complement(18976..19383)
                     /gene="gloA"
                     /locus_tag="QBF46_001730"
     CDS             complement(18976..19383)
                     /gene="gloA"
                     /locus_tag="QBF46_001730"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDQ6673919.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAFGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(19464..20561)
                     /gene="nemA"
                     /locus_tag="QBF46_001731"
     CDS             complement(19464..20561)
                     /gene="nemA"
                     /locus_tag="QBF46_001731"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDQ6673920.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPDEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(20598..21197)
                     /gene="nemR"
                     /locus_tag="QBF46_001732"
     CDS             complement(20598..21197)
                     /gene="nemR"
                     /locus_tag="QBF46_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDQ6673921.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            21300..21539
                     /locus_tag="QBF46_001733"
     CDS             21300..21539
                     /locus_tag="QBF46_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDQ6673922.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            21588..22484
                     /locus_tag="QBF46_001734"
     CDS             21588..22484
                     /locus_tag="QBF46_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDQ6673923.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            22565..23086
                     /gene="sodC"
                     /locus_tag="QBF46_001735"
     CDS             22565..23086
                     /gene="sodC"
                     /locus_tag="QBF46_001735"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDQ6673924.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(23087..25099)
                     /locus_tag="QBF46_001736"
     CDS             complement(23087..25099)
                     /locus_tag="QBF46_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDQ6673925.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTTLASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(25099..25956)
                     /locus_tag="QBF46_001737"
     CDS             complement(25099..25956)
                     /locus_tag="QBF46_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ6673926.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(25959..26195)
                     /locus_tag="QBF46_001738"
     CDS             complement(25959..26195)
                     /locus_tag="QBF46_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDQ6673927.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            26396..26830
                     /gene="slyA"
                     /locus_tag="QBF46_001739"
     CDS             26396..26830
                     /gene="slyA"
                     /locus_tag="QBF46_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDQ6673928.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(26877..27344)
                     /gene="slyB"
                     /locus_tag="QBF46_001740"
     CDS             complement(26877..27344)
                     /gene="slyB"
                     /locus_tag="QBF46_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDQ6673929.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            27618..28727
                     /gene="anmK"
                     /locus_tag="QBF46_001741"
     CDS             27618..28727
                     /gene="anmK"
                     /locus_tag="QBF46_001741"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDQ6673930.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSCNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            28825..29154
                     /gene="mliC"
                     /locus_tag="QBF46_001742"
     CDS             28825..29154
                     /gene="mliC"
                     /locus_tag="QBF46_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDQ6673931.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            29213..29869
                     /gene="pdxH"
                     /locus_tag="QBF46_001743"
     CDS             29213..29869
                     /gene="pdxH"
                     /locus_tag="QBF46_001743"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDQ6673932.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            29998..31272
                     /gene="tyrS"
                     /locus_tag="QBF46_001744"
     CDS             29998..31272
                     /gene="tyrS"
                     /locus_tag="QBF46_001744"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDQ6673933.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            31331..32194
                     /gene="pdxY"
                     /locus_tag="QBF46_001745"
     CDS             31331..32194
                     /gene="pdxY"
                     /locus_tag="QBF46_001745"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDQ6673934.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(32238..32843)
                     /gene="gstA"
                     /locus_tag="QBF46_001746"
     CDS             complement(32238..32843)
                     /gene="gstA"
                     /locus_tag="QBF46_001746"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDQ6673935.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(32949..34451)
                     /gene="dtpA"
                     /locus_tag="QBF46_001747"
     CDS             complement(32949..34451)
                     /gene="dtpA"
                     /locus_tag="QBF46_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDQ6673936.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(35062..35697)
                     /gene="nth"
                     /locus_tag="QBF46_001748"
     CDS             complement(35062..35697)
                     /gene="nth"
                     /locus_tag="QBF46_001748"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDQ6673937.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(35697..36392)
                     /gene="rsxE"
                     /locus_tag="QBF46_001749"
     CDS             complement(35697..36392)
                     /gene="rsxE"
                     /locus_tag="QBF46_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDQ6673938.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(36396..37016)
                     /gene="rsxG"
                     /locus_tag="QBF46_001750"
     CDS             complement(36396..37016)
                     /gene="rsxG"
                     /locus_tag="QBF46_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDQ6673939.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(37020..38078)
                     /gene="rsxD"
                     /locus_tag="QBF46_001751"
     CDS             complement(37020..38078)
                     /gene="rsxD"
                     /locus_tag="QBF46_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDQ6673940.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(38079..>38208)
                     /locus_tag="QBF46_001752"
     CDS             complement(38079..>38208)
                     /locus_tag="QBF46_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HDQ6673941.1"
                     /translation="LEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         9626 a         9597 c         9952 g         9033 t
ORIGIN      
        1 tagcaatttt tttcattgtt acttacctgt acgcattctt tcgaatttag tgttatcaga
       61 taagtttggt tgagattttt agaatttgag aaatttcaga cagggatgaa aagggcgcta
      121 accgcccttg attaaattct gtcaggcgag ttttgcctga ttgatttgac taagtggctc
      181 attgacatcg aaaactttgg cgacaacttc ctcaacgccg agatttttca gtcgggcagt
      241 gtgtttttcc agataggcaa gggcgctttt ttcatcagtg aaaaggtaga tcccaccggc
      301 ttcgtggttc ttttcacttt ctgtccatac cttccacaga aaaccaggtt cctgattaat
      361 cgactcagca agtggtttaa gctgctcagc cattgcgtcg ccaaatgggc cattaaaagc
      421 aaaatgaagt tgtaacaaag ttgccatagc gattctcctt ctgataacgc atacgaagac
      481 agtgtaaatc ttttaacgta ttggatacag agaagaaaca gagggaaaaa ggagaagacc
      541 agattaagaa aatctggtgc gtccgagtgg actcgaacca ccgaccccca ccatgtcaag
      601 gtggtgctct aaccaactga gctacggacg caggatggtg cgttcaattg gactcgaacc
      661 aacgaccccc accatgtcaa ggtggtgctc taaccaactg agctatgaac gcaacgttgt
      721 aggtgacaac ggggacgaat attagcggca gagtgggaag gtggcaagag gcaaaacgta
      781 attttctgcg ctatttcgac cgtttgcaga gcttttaagc aaattggcta tattttgttg
      841 atttgcaagg gcgattttta ttcaggatcg catttacatc tgatacaacc caataattcg
      901 tgtatcagag tttaaacaga gttgtttgcc accatctcaa atctgtcgtg gataaatcct
      961 ccgtagcctt tacacttagg tagcaataaa ggcacacaga tgcagcaaag gaataaatca
     1021 tgggatctga tgcgaaaaac ttgatgagcg acgggaatgt gcaaattgtt aagaccggcg
     1081 aggtcattgg cgcgacgcaa cttactgaag gcgagttgat tgttgaagct ggcggaagag
     1141 ccgaaaatac cgtggtcacg ggggctggct ggttgaaagt ggcaaccggt gggatcgcca
     1201 aatgcacaca gtacggtaac aatggcacgc tatcggtcag cgacggtgcc attgccacag
     1261 atattgttca gtccgaggga ggcgcaatta gtctctctac gctcgctacg gttaatggcc
     1321 gccatcccga aggtgaattc agcgttgata aaggttatgc ctgcggtttg ttgctggaaa
     1381 atggcggtaa cctgcgtgta ctggaaggcc atcgcgcgga aaaaatcatt ctcgatcaag
     1441 agggcggcct gttggttaat gggacaacct cagcggtcgt ggtagatgaa ggtggtgaat
     1501 tgttggtgta tccaggtggg gaagccagca attgtgagat taatcagggc ggcgttttta
     1561 tgctggccgg gaaagccagt gatacgttgc ttgctggtgg caccatgaat aatctcggtg
     1621 gtgaagactc tgacactatt gttgagaatg gatccattta tcgtctgggg acggatggcc
     1681 ttcagctcta cagttccggt aagacgcaaa acctgtccgt gaatgtgggt ggtcgggctg
     1741 aagtgcatgc cggtacgctg gaaaatgcgg taatacaagg tggaacagtg atcctgttgt
     1801 cacccaccag cgcggacgaa aattttgtcg tagaggaaga tcgcgcaccg gttgaactga
     1861 ccgggagtgt tgcattactg gacggcgctt caatgattat tggttatggc gcagagctgc
     1921 aacaatcaac gattactgta cagcagggcg gtgtgttgat tctcgacggc agtacggtaa
     1981 aaggtgacag tgtcactttc agtgttggta acatcaatct gaatggcgga aaactgtggc
     2041 tgatcactgg tgcggcaacg catgtgcaac tgaaagtgaa acgcctgcgc ggagagggag
     2101 cgatttgcct gcaaaccagt gcgaaagaaa tttcacctga cttcatcaat gtgaaagggg
     2161 aagttaccgg tgatatacac gttgagataa cagatgccag tcggcaaact ctgtgtaacg
     2221 cactgaaact acagccagac gaagacggga ttggcgcaac gctccagcct gcgtaaaaat
     2281 acaggcgcaa cccgtgaagg ttgcgccttg tctttattag cgggacgctc gttgcagaat
     2341 gatggctgac ggcatacgtt gcaggaaccg catacgcagc atcatcataa tggctgccga
     2401 cgtcaggcca ataataaagc ctatccagaa gcctgctggc cccataggtt caacgaccag
     2461 atcggttagt gccagaatat agccgcttgg caagcccagc acccagtaag ccgtaaaggt
     2521 gatatagaaa atggaacgcg tatctttata accacgcaaa atcccactgc caatgacctg
     2581 gattgagtca gaaatctgat ataccgccgc cagcaacatc aaatgcgcag ccagcgttac
     2641 aacctcggga ttgtcgttgt acaacagggc gatttgctcc cgcagtgaaa ccgtgaatat
     2701 ggccgtcagg gttgccatac agacacccac cataagcccg gtccgcgcag cggtttgcgc
     2761 atccagcgtt gagccctgac ccagacgata acctacgcgg atagttaccg ctgccgccag
     2821 cgacattgga agcacaaaca tcagtgaact aaagttcagg gcaatctggt gtcctgcgac
     2881 atcaacaata ccgagcggag acactaacag agccacgacg gcaaacagtg tcacttcaaa
     2941 gaacagtgcc agcgcaatcg gcaaaccgag ttgaatcagt cgtttcataa ccgcaggatc
     3001 gggtttcgcg gtgccttttt cgttacggat atcgcgcatg gagcgggcgc gtttaatgta
     3061 agaaaccatg gcaaggaaca tgacccaata caccgccgca gtagccacgc cacaaccaac
     3121 gccaccgagc tcaggcatac cgaaatgacc ataaataaag atatagttca ccggaatgtt
     3181 caccagcagg ccgataaagc ccattaccat acccggcttg gtttttgcca gaccttcaca
     3241 ttggttacgg gcaacctgga agaaaagata gcctggcgcg ccccacagca acgcacgcag
     3301 ataacccacg gctttgtccg ccagagccgg atcgatgttt tccatggagc ggataatgta
     3361 acctgcattc cacagcacca gcataatgag aacggaaaca aaacctgcca gccagaaacc
     3421 ttgtcgcacc tgatgcgcaa tgcgctcacg tcgaccggaa ccatttaatt gcgcgataac
     3481 cggcgttaat gccagcagca gtccgtgacc aaagaggatc gccggaagcc agatagaagt
     3541 accgatagcg accgccgcca tgtcggtggc actgtagccg cccgccatca cggtatcgac
     3601 aaaacccatc gcagtttggg cgatttgcgc gagaatcacc gggattgcta atgctaataa
     3661 cagacgcgct tcactgatat acttctgcac gtgaacacct tttatttgta gttatatgag
     3721 agactaaaaa gccgcctgaa tgggcagcaa aaagaagaag caggggaaat tcagtctatt
     3781 gtagcgagga tttattaatt ctccagtgaa aaaatagcca ttcttacccc ttcactggta
     3841 agggcgtttt ccacctgcta ttgtgctgaa cagaatgtct taactgattt caggagttgt
     3901 aagtatgttt acggggattg tacagggcac cgcaaaactg gtgtcgattg acgagaaacc
     3961 aaattttcgt acgcatgtgg tggagttacc cgaccacatg ctggacggcc tggaaaccgg
     4021 tgcttccgtg gcgcataacg gttgctgcct gaccgtgacg gaaattaacg gcaaccatgt
     4081 cagttttgac ctgatgaaag aaacgttacg cattaccaat cttggcgatt taaaagtggg
     4141 ggattgggta aacgttgagc gtgcggcgaa attcagtgat gaaattggcg gacacttaat
     4201 gtcaggtcat attatgacca ctgctgaagt ggcgaaaata ttaacctcag aaaataatcg
     4261 ccagatctgg tttaaagtcc aggatagtca gttgatgaaa tatattctgt acaaaggatt
     4321 tattggcatc gacggtatta gcctgaccgt cggcgaagtc acgccaacgc gtttttgcgt
     4381 ccatttaatt ccggaaactc tggaacgcac gactcttggg aagaaaaaac tcggcgcacg
     4441 cgtcaacatt gaaattgatc cacaaactca ggcagtggta gatacggtag aacgtgtgct
     4501 ggcggcacga gaaaatgcca tgaatcaacc aggtacagaa gcctgatctg aaatcgcccc
     4561 cggagagggg gcgatagaat agcctttagc gagcgactcg aaggccgttt tcaacaccgc
     4621 gtgagaacac gacctgccag agctgaatat cacgggcgcg gaaagcacct gcacaggcat
     4681 tcagataata ggtaaacatt cgtttaaagc gttcactgta gttatccgca atttctggcc
     4741 atgcggcgag gaatcgttca taccacgcca tcaacgtagt atcgtaatca gcaccgaagt
     4801 tatgccagtc ttccatcaca aagtggggtt cgctggattg agcaatctgg cgtacagagg
     4861 gcaggcaacc gttcggaaaa atatatttat taatccaggg atcaacattc agatcggttt
     4921 ttttcgaacc gatagtatgg agcaggaata taccttccgg tttcaaatta cgatccacca
     4981 ccgcaaaata ggtatcgtaa tttttcggtc cgacgtgctc gaacatcccc acagaaacaa
     5041 tacgatcaaa ctggtcgttc aggtcacgat aatcttgcag caaaatggtg acatccaggc
     5101 cttcacagcg ttcctgagcc attttttgct gttcggcaga aatggtgacg ccgaccacgc
     5161 ttacgtcata gttagatgcc atgtagtgcg ccagtccgcc ccagccgcag ccaatatcca
     5221 gtacgcgcat ccctggtttt aactgcaatt tttcacaaat cattttgagc ttcgcctgct
     5281 gggcagattc cagattatcg gcatctttcc agtaagcgca ggaatattgc atgaagggat
     5341 caagcatgcg gctgaacaag tcattaccca aatcgtaatg ctccttgccg actatccagg
     5401 cacgtttttt actctgcaga ttgaagagac gagcgctggc aatacgcagc gtgtctttga
     5461 aatgatgggg gagttggttc tcgagacctg cgcgtaagac tttgctaaaa aacatatcca
     5521 gtcggtcaca ttcccaccag ccatccatat aactttcgcc taaccccaaa gagccttctt
     5581 gcagaacgcg tttaaaaaaa tcggggtttt tcacacgaat atccgccggg gcagaaccgt
     5641 taatggctat accggcacgg ctaagtaatt cgttggcgat acggtaccag ttgtcatccg
     5701 gtacactgac ttcttctata cacgatgaac tcatagtttc tccatcactg gtcggtgatc
     5761 agaaccttaa aacagcgtag acgctttttg ggctttgtga gaaatctcgc agaaaaaacc
     5821 gcgagtctat aatccgacgt agaacagagg gaaaggagga acccttgccg aaatgaccat
     5881 ccatagtgag tcgggaacga tcctgtcccc gtaaaaacat aatatttatc gtagctttaa
     5941 caactcgaaa ttagtatagg ccgctaattt caatcataca acaaaatttt gttagcagcc
     6001 taagtataag tatatcgata tagatcagtg tgattcgcta tgagcgactt cggcattgcc
     6061 atgattctgg cagccaactt cttcacaacg ttgcatcatg taacccagcg cgaccagcac
     6121 tactgttgat aacatcacgc tggtggtggt gagcaatggc gtgctgatac tgatcagcca
     6181 ggaaactacc agacttgcga ggaagcacag acccagttgt agagtgttct gcaacgctgc
     6241 ggcgcgacca gttgcgtgtg ggaagggacg cagcgcctgg gcgacaacaa tagggtagat
     6301 cgcgccattg gcaatcgcca tcacacagaa tgggatcagg atttcgacca gcgacacatg
     6361 gctaatgaag cctgcagccc aggtcgcaat gacgctgact gcaaacagca ccagcaacca
     6421 cggtaataac tgcttgcctt gccatttctg cagcgcggcg cgacagccat aaccaccaat
     6481 cagaaacgcg atagtttgcg ggacataact taaaccaata actgccgggc tgtagcccat
     6541 ttcactaagg atgaacggtg aaccggtcag ccatgcaaaa aaactggctg aacaggccgc
     6601 gtatatcagc acgttgccgc gataggtttt agaacgtagc aggtcggtaa aggtcagacc
     6661 atcctgacta ttgttacggg ccttcgtcgt gggtttgagc cagaaaatag gcagaatcag
     6721 caccacggta atggcaaaca gggtggcgaa aatcgcctgc caggaaaaat ggaccagcag
     6781 ccagcttcct aacagaggag ccagggccgg agatagaccc accagcggca tgatggtcgc
     6841 aaaaattcgg ttaactttct gtgaaggata ataatccgtc actaacgctt gccagataac
     6901 cgccgcggcg cagaccccca cagcctgtac aaaacgcaat accagcagcg tggcggcgtt
     6961 ttctacccac agcatcccca gactacctaa cgcaaaaatt gtcaggccga ttaataacac
     7021 cggtttacga ccataacggt cggagagtgg cccccacaga agctgggctg cggcaaaacc
     7081 ggcaaggaac agactaaggc tggcactgac agcagacgca ggcgtttgca ggtcggcctg
     7141 tatggcggcg aaagcaggca gatacatatc ggttgccaga aaaccgagta cgctcaaacc
     7201 cgccagccag actaaaaatc ttttcccagg ttgcatgtat attctctaaa tctctcaatt
     7261 aaacaccgcc gcaaagtgta gggagtgaaa ttagtgttgt gaaacggtaa tatttgatga
     7321 ctggtttcaa aaattttgca ggcagactat gtggtcagaa tattcactcg aagttgttga
     7381 tgcggtagcg cgtaatggta gttttagcgc tgcggcacag gagttgcatc gcgttccttc
     7441 tgcggtcagc tataccgtgc gtcagctgga agagtggctg gcggtgccgc tctttgaaag
     7501 acggcaccgt gatgtggaac tgaccgctgc cggagcgtgg tttctcaaag aagggcgctc
     7561 tgttgtcaaa aaaatgcaga tcacccgcca gcaatgtcag cagatagcga acggctggcg
     7621 cggtcagtta gctatcgcag tggataatat tgtcaggcca gaacgtacac ggcagatgat
     7681 cgttgatttt tatcgccatt ttgatgatgt cgaacttctt gtctttcagg aagtgttcaa
     7741 cggtgtctgg gatgcgcttt ccgacgggcg cgtggaactg gcgattggcg caacacgcgc
     7801 gattccggta ggcggtcgtt atgccttccg ggatatgggg atgctaagct ggagttgcgt
     7861 tgttgccagc catcacccgc tggcgttgat ggatggcccg ttcagcgatg atacgttgcg
     7921 caactggccg tcgttggtgc gcgaagacac ctcgcgaacg ctacctaagc gtattacctg
     7981 gttgctggat aaccaaaaaa gacttgtcgt gcctgactgg gaatcatcgg caacctgtat
     8041 ctcggcagga ttatgcatag ggatggtgcc aacacatttc gccaaaccgt ggcttaatga
     8101 ggggaagtgg gtagcattag aactggaaaa cccctttccg gattcggcat gttgtctgac
     8161 atggcagcaa aatgatatgt cgcctgcgtt aacctggttg cttgagtatc tcggcgatag
     8221 tgagacgctg aataaggagt ggctgcggga gccggaagag actcccgcaa cgggtgatta
     8281 acgacgatag tcgcggaacg ggccgtcagc cacggagcgg cgttcaatca agcgcggatg
     8341 cacttcaatg gactgcggtt cttcacgttt gttgacgata cgatccaaca gcatgttgaa
     8401 cgctgtttca cccagcgagt cttttggctg atggatcgtg gtcagcgccg gcgtaaaata
     8461 gcgggcgttg cgcacgttat cataaccgat cagcgaaaca tcctgcggga cgcgcaggcc
     8521 catttcatca gcagcacaaa gtgcgcccat tgccatgata tcgccaccac agaagacggc
     8581 agtagggcga tgcggctgcg acaggatttg ctgcatggcg cgataaccgg attcaggttc
     8641 aaaatcaccc tgcacaatcc agctttccgg caccttgatc attgcttctt ccatcgcctt
     8701 cataaaaccg gcaaggcggc ctgcgccagt gttacgttcc agcgggccgg ggataacgcc
     8761 gatttcgcgg tgaccgcgtt caatcagata acgcccggcc atgtagccgc cttcgaacgc
     8821 gttatcaatg accgcatcgg tgaagtcagc ttttgcttca ccccagtcca tcaccaccat
     8881 tgggatatgg cgatactctt ccagcatcgc cagcaacggc tctgggtact cagaacacat
     8941 caccagcaga ccatcgacgc gtttttgcgc catcatcgac agataagccc gctgtttctc
     9001 aagattgttc cacgcattac ccagaatcag ggtgtaacct ttctggaagc aatttttttc
     9061 aaccgcttca atgatctcgg caaaataggc cgcttcgctg ctggtcgcca gcaaaccgat
     9121 agacttggtg tggttaacct tcaggctacg cgccaccgcg ctaggggagt agtgtaattc
     9181 tttaatcgct gcccacacgg cgttgcgcgt ttcttcagcg acgaaacgtg ttttgttgat
     9241 cacgtgtgac acagttgtag tggaaacgtt tgctcgtttc gctacatctt ttattgttgc
     9301 cattccattt cactccagac cctaacttca gctcctgcaa aatcgcaagg taaacgtttg
     9361 cctttacaca ccttacgcac aacaaaagtg ttgcggtacg ccggaaaaac gacacggagc
     9421 gtcaggaagg ggtcaatggc cagtacgcaa atagtgtaag cgtggaattt tggccgatct
     9481 tgacgaaaag gggaagtggt aaaaacactt atcattataa atgacgggag attttttcat
     9541 cacagtgtgt aaaaatgcgt aatatcactt tttgtgtggt aataaaaaag gagaaaactt
     9601 gatgagcacc gaccttaaat tttcactggt aacaacgatt atcgtcctcg gtttgatcgt
     9661 agccgtgggt ttgactgccg cgctgcactg atttcctctc gagggagcgc gttctccctc
     9721 gcatctcctt cctgtttgtt agcaaaatgg caaaagtgtt ttctcttttt gttattttca
     9781 tttttttgtg attgctgtca tgctttcgcc actttaatga atatcgcgta atgacgcgaa
     9841 gaggtggaga taaggcatga aaattaacta tccgttgctg gcgctggcga ttggcgcgtt
     9901 tggtatcggg acaacggagt tctcgccaat gggcttgttg cccgtcattg cgcgcggtgt
     9961 ggatgtctcg attcccgctg ccggaatgtt aatcagtgcc tatgcagttg gcgtaatggt
    10021 gggcgcgccg ctgatgacgc ttctactttc tcatcgtgcc cgccgcagtg cgttgatttt
    10081 cctgatggca attttcacgc taggcaacgt tctttccgcc atcgcgccgg attatatgac
    10141 cctgatgctt tcacgcattt tgaccagcct gaatcacgga gcattttttg gtttgggttc
    10201 agtcgtggcc gcaagcgtgg tgccaaaaca taaacaggcc agcgcagttg ccactatgtt
    10261 tatggggtta accctggcaa atatcggtgg cgtgccggcg gcgacctggt tgggtgaaac
    10321 catcggctgg cggatgtcat ttctggcaac ggcggggctg ggagtgattt caatggtaag
    10381 tctgttcttc tcattaccta aaggtggtgc aggggcacga cctgaagtga aaaaagagct
    10441 ggcggtatta atgcgtccgc aggtgctgtc tgcattgctg acgacggtac tgggagctgg
    10501 tgcaatgttt actctctaca cctatatctc tccggtactg caaagtatta cccacgcaac
    10561 accggtgttc gtcacggcaa tgctggtgct gattggtgtc ggattctcta tcggtaacta
    10621 tctcggcggc aaactggcag atcgttcagt taacggcacg ttgaaaggct ttttgttgct
    10681 gctgatggtg attatgctgg caatcccgtt cctggcccgc aatgagttcg gcgcagctat
    10741 tagcatggtg gtgtggggcg cagcaacctt tgcggtcgta ccgccgttac agatgcgcgt
    10801 gatgcgtgtc gccagtgaag cgccgggtct gtcttcatca gtcaatattg gtgcctttaa
    10861 tcttggaaat gcgctgggag cagctgctgg tggtgcggta atttccgctg ggcttggata
    10921 cagctttgtg ccggtgatgg gagcgattgt cgcgggactg gcattattgc tggtgtttat
    10981 gtcagccaga aaacaacctg aaacagtttg cgttgctaac agctaattaa acaaaaacgc
    11041 agaaggataa ccttctgcgt tacagttgct gacaacgtgc gatttatctt gccggatgcg
    11101 gtgttaacga ctcatccgcg ttgacatgaa aagacgcgtt aggcgtcgta tcatgcagcg
    11161 cttgtcggat gcggcgagtg ccttatccga cctacatcga tgatccgcgt aggcctgata
    11221 agcgtagcgc ttcaggcaat gttgcatttg ccatcagtta ttatgcagcg agatttttcg
    11281 ctacgaattc ccagttcacc agcgcccaga agtgctccag atagccagga cgtgcattgc
    11341 ggtagtcgat gtaatatgcg tgttcccaga catcaacggt cagcagcgga gtcgcatcgg
    11401 tggtcagtgg agtacccgcg ttagaggttg aaacgatagc cagcttgcca tcgctatttt
    11461 tcaccagcca ggtccagcca gaaccaaagt ttttgatcgc tgcatcagta aactgagctt
    11521 tgaaatcggc aaagctgcca aaagaagcgg tgatggcgtc agccagttca cccgtaggtt
    11581 cgccaccggc gttcggtgcc aggcagttcc agtagaaagt atggttccag acctgagctg
    11641 cgttgttgaa tacgccacct tctgagctgc gaataatctc ttccagtgat ttaccttcaa
    11701 acgcggtacc tttgatcagg ttgttcaggt tagtgacata agtctgatgg tgcttgccgt
    11761 agtgatactc gatggtttcc gcagaaatgt gcggtgccag agcatcttta gcatatggta
    11821 gtgcaggtaa ttcgaatgac attgctactc tcctttatta ttaatttgca tacgtacaat
    11881 agccttattg tgcgtatgga tagggtagca aattaaaagg tgagacaaaa gatgaactta
    11941 ccctgttgcc gtaacaacag ggtaaagttt tagcgaagtg tttttggggt cattacccga
    12001 cgagcgccaa catagtggcg ctgccagtag tcttcactga gagaagtgat ttgaatttcc
    12061 tgacctgtgc gcggtgactg aataaatttg ccgttgccga catacacgcc gacatgatcg
    12121 gctgtgccgc gtccctgagt acggaaaaag accaggtcgc cgtttttcag ttcactacgt
    12181 tcgattggcg ctgcatcacg caggtgatac atttcattcg ccgtacgcgg aatacgaatt
    12241 ttcaccaaat ctttataagc gtaataaacc aggccgctgc aatcaaaacc ggtacgcggt
    12301 gagctgccac cccaacgata tggcttacca atttgctgca tcagtttatt cattgccact
    12361 ttcgtagctt tttgcacctt cgctttgtgc gcgtcggcaa gagttcctga cgcggcattt
    12421 ttgactttca cacaatgcga tttatagccc ttacgtttgg tacacttttc ggtaaagctg
    12481 gctgaggccg ttttattgac ggcgtgtttg ctgcgagttt tggcgttttt agaagcggta
    12541 atggaagatt ttttaacgga agaagcggtt ttgcttttgg tcgtactttt actggcggct
    12601 tttttcgctg ttttctgggt ctttttggtg gttgccgtgc ttttcttttt atctgctttt
    12661 ttggtgatat gagtggtagc agaactctcc ctggcttgct ttgaagcatg ggccataggc
    12721 gttaaaggca gggtggtaaa aagtaaagca cagagcgtga tcgaaatacg gtttatccgc
    12781 gccactgagc gtccctctgt taaggcaggt ctatcatgcc tgcgtctgat ttacaaaact
    12841 tgatgattct aatgcataga agaagagaaa gttaatagct tttattgtta aaaaatacgt
    12901 ttcgttggcg agcgcaaaaa aatgcagcag tgaaagctaa aaatggttat aaatacgcca
    12961 atttgccggc atcagagaag aaacgaatat ttgaaatgca acaaatagag caaagggata
    13021 aaatagcaaa agcgtgatga aaatgttatt ttccgatgcc gcactgaccc gctacaatgt
    13081 cagattactg ccgtaaagaa gtttgaggaa gcaagacaat gagcaccact atcgaaaaaa
    13141 tccaacgcca gattgctgaa aacccgatcc tgctgtacat gaaaggttca ccgaaactgc
    13201 cgagctgcgg tttctctgcc caggcagtcc aggcgcttgc cgcatgtggc gaacgttttg
    13261 cctatgttga tattctgcag aatccggaca ttcgtgcgga actgccgaaa tatgctaact
    13321 ggccgacctt cccgcaactg tgggttgacg gcgagctggt cggcggttgt gatatcgtga
    13381 tcgaaatgta tcagcgtggc gaactgcagc agctgatcaa agaaactgcc gctaaataca
    13441 agtctgaaga gccggacgcg gaataatccg tcctgcaata ctgacggatg cgaacgggca
    13501 tccgtcagtc ctttctctat ccccaatcca gcccttgtgg cgaacttgaa aatcccgcct
    13561 cacgcagcag cgtaaacatc ggcgtttgcc ggaccggtag atcattcact tctgttagcg
    13621 taaagcgcag ccgtggttcg cgacgcagtg cggtattcag cgcgtggaaa acctctgggg
    13681 cgagtaattc ctctttttcc tgccagacca gcattttttt gccaccttgc gccagataga
    13741 gtaacaattt gccgccagaa acgaccacca gcgcacccgc ccgacgcgtt ggaaccagcg
    13801 ttgccggatg tgcaggccag ggcagaagat ttccccacac attagccgga tcgttggcag
    13861 agagcgccac tggtgtatag tggcgcgttt gcgtggcttg tgtcgccaga tcgcgcaatc
    13921 ggtcaatagt cagacgttca gcgaattgcg cgccacccag accttctaca aaacgacctc
    13981 gcataattcg cccggagtct tccatacttc gacaaagcgt ttgcatcgat ggaaacccgc
    14041 cagggatatt ttcggctatc accgcctgac gactgatgat gccgtagcgg tcgagcatat
    14101 tttccgccag cgccagcatc ctttcggtat cgtttagtgg ctccacctgc aataacgacc
    14161 agcgtccagc cagatttggt gtgttgtaag atacccgcgg cgagacgggt tgcgcataga
    14221 caggacgtcc acgacgagcc cggtgactgc ggcgagttga ggtgcgtgcg ttggaactgc
    14281 tgcgggtgag ggcgcgtaac ggtgcccaaa tgtcgctggt gatgacgcct tgccagacca
    14341 gcgcccataa cgcctcttgc agggcagaga gatcaaccga ttcgccgatt ttgtcgcgta
    14401 tccgttggct gatttgttgt gcaaaccagg ctcctccgtc agccagaaca gcgactatcg
    14461 cttgttgcag cgccgaacga ttcgcctgat ccgcttcggc gggagtgaac gattctgcag
    14521 catattcctg tagatgcagt gccaccaggc cgtcatcttc acccagtttt ttttgccccg
    14581 accagataac cgcaccggtt gccagtaatt catcgagcat ttctgacgaa tagtcgcgta
    14641 cgcgggcagg caggatctgg ctttcccaga gtgaggcggg taaaccgact ccggcaagct
    14701 gttcgatcac ccgcatcacg ccatctacgc cctcataaac gcctggcgat gttgaggcaa
    14761 agagcgccgg gctaccatcg gtggcgggta atacgccctg acgttccagc agcaatcgcg
    14821 cataggtcgt ggctgcaacg ggacgcgtcg cttctctggc ggcttgcagc gagcgcaaac
    14881 gcagacgacg aaatacttca tcgctcaccc agatgtcttg ttgcagattc atcaccagac
    14941 cctgttcacg cagttgctga agctgctctt cgacaatggc aataccgaga ctaaactcat
    15001 gagccagttg ttcagccgtg accagagcat gagcccggag atagcgcaga aagaggtcgc
    15061 gaagcgggta actgactcta tgtaaataaa tctctggcaa tgactctggt agtcgtacgc
    15121 cgagggcatc acgcagcctg gcggcatcat ccatacaggc aagacgctcc tgtccgctaa
    15181 tcatcgctgg gaagattcgt tttactgcaa gaagattttc cagatacgac gcaacctctt
    15241 cactgctgcc tgtatgccgt tgcgccaggt cttcaacggt cattggcccc agttcgcgca
    15301 gcaggtcgaa caatccttct tcacctttcg ctcttctgcc aggagccagt cgttgcaact
    15361 cttcttccac ctggcgaatg acctgcgggt cgagtaattc ccccggatcg acctgtccga
    15421 gtagattgcg cagtaactcg ctgtccagcg acagtacgga tgcccggcgc tctgccagcg
    15481 gggcgtcgct ctggtacata aattccgcga catagccgaa caataaactg gtggcgaaag
    15541 gcgagggcgt agtagtcgtt acatcggata tttgaatttc gccacagttc aggcgacgca
    15601 tcaaacgttc cagtgcggga agatcataaa catcttgcag acattcgcgt agggtttcga
    15661 gaatgaccgg aaaatccgga tatccctgag cgatttccag caactgactg gcgcgcagcc
    15721 gttgttgcca aagcggggtg cgatggcccg gcgtgcggcc cggcattaat aatgcccgcg
    15781 cggcgcattc gcgaaaacgg gcggcgaaaa gtgccgaact gcctaccgcc tcgcggacaa
    15841 tttgcagcaa cttttctggt tcaaacaaaa aaatcgcggc atcgggcaat ttaccatcgg
    15901 tgtcaggaat acgtgcaaca atgccgtcat cactggcgac caccgacgcg tcagcgcccc
    15961 atagggcatg tattcgcccg gcaatcgcca gcgcccaggg ttcatgcacc cgtcttccat
    16021 agggagagtg caaaataata cgccagtcac caatttcatc acggcaccgt tcgaggacca
    16081 gatggcgact ccccggaacg atgcccgtcg cgttgcgctg ctcctcaatc aacccctgaa
    16141 tattggcgat cgtattttct tctgccagcc acgggggaat agtcccggaa aagaacgcgc
    16201 catccgccag caaatgaaga aaatcgccga tcatctcgcc taattcagcc ggacgtccgt
    16261 taccttcacc acgccagaag gggagccggg cagaacgacc cggagcagga gtcacaatca
    16321 cctgatcgcg ggtgatttgc tggatccgcc atgaggtagc gccgagagtg ataatgtcgt
    16381 tcacccgcga ctcatatacc atctcctcat ccagttcacc cacccgccgt gaaccggcct
    16441 tttcttcacc ttcgggtaat aacacgctat acatgccacg atccggaatg gtgccgccgc
    16501 tggtaaccgc caacaattga gcgccaggtc gggcggtcaa tatcccggtc tccctgttcc
    16561 agaccagttt ggggcgaaaa gcagaaaaat cgccagaggg atagcgcccg gaaagcatat
    16621 ccagcgtggc gtcaaaaaca cgtcttggca gatctttcca cggtgcggca cggcgtacgc
    16681 gggagtacca ttcgtctacc tgtaatgcat ccatcgccgc ggcggcaacg gtttgctgcg
    16741 caaggacgtc gagaggatta tgcggtggtg tcaggttttc cagcctgcct gcgaacatac
    16801 actctacaat gactgcggaa tcgactaaat cacgccgggt acgggggaaa aacagccctt
    16861 tagatacacc gccaacctga tgtccggcgc gaccaatgcg ttgtaaccca ctggcaacag
    16921 aaagcggcgt tgccacctga atcaccagat ccaccgcgcc catatcaatc cccagttcaa
    16981 gactggaggt tgcgaccacg cagcgtaatt cacccgattt cagcgcctgt tcggtgattg
    17041 ctcgttgttc tttagagacg gagccgtggt gtgagcgggc aataaaaacg tcgctacttt
    17101 gtacacggtt agaggttgcg ccggaggtcg actcgaaatg ggccgcatca acggcgatag
    17161 acggggaacg ctgtaagcgt gcggcgtaaa gctcatttaa tcgtgccgtc agtttttccg
    17221 ccagcccccg cgaattagta aagacaatgg tcgagcgatg gcgcaacact tcatcaagga
    17281 tgcccgtttc aatatatggc cagatggagc cttcccggcc ggcatggctg tcttcgccag
    17341 tgccgctggc gaccgatgag acatcatcca tattggcgac cggtacgaca atccgtatct
    17401 gcggatgacg cattgcgggc gggttgacta ccgtaaccgg gcgatcgcca ccaagaaatg
    17461 ctgccacatc gctggctgag cgcacagtgg cagaaaggcc aattcgctgt gctgaggtgt
    17521 ggagcagcgc atcgagccgc tccagactta acgccagatg cgcaccacgt ttactgcccg
    17581 ctaccgcgtg gacttcatca ataattaccg tttcgacgcc gcgtagcgtt tcgcgcgcgc
    17641 gggaggtcag catcagatag agtgattcgg gtgtggtgat cagaatatcc ggcggattac
    17701 gggtgagttt gctgcgttcc tgtgcaggcg tatcgccagt acggatccct acgcgaagat
    17761 tgacttccgt ttcgccgcgc cgccgccgtt catcggcaat acccttcaac gggatctgca
    17821 agttgcgctg aacgtcggtg cccagggctt ttatcggtga aatatagagg atgcgtgagg
    17881 ttttacgctt atgcgcctcg cgggtatctt cgccgccttc gcggaagagc cgatcgaggg
    17941 cgtagagaaa tgctgccagc gttttcccgg agccagtcgg tgcaatcacc agcgcatgtt
    18001 cgcctcgcgc cgccacatgc caggtttgcg actggacagc ggtcggctgt ttaaaggcgc
    18061 gaagaaacca ttcgcgggtc gccggtgaaa acacgtccgg caggagcgat gaagggtctg
    18121 gattatctgc cattaacgct attccccgtg aatatcatgc aggatgcgtg gaatagcctc
    18181 actatagatg cctgaatcat tatatgtcat caggcatcga ctcgattaca cctcttcggc
    18241 ggtgggtagc ggccagcctc ccagacgttt ccagcggttg acgatttcac aaaacagcac
    18301 agcggtgcgt tcggtgtcgt acagcgcgga gtgcgcctgg gtgctgtcga agtccatgcc
    18361 agcggtctgg caagcctttg acaatacggt ttgtccgagt gccagcccgg ccagtgcagc
    18421 agtgtcaaaa gtggcgaaag ggtggaacgg gttacgtttc agcgaggcgc gttctgcggc
    18481 ggccatcata aagctgtgat caaaattggc gttgtgcgcc accataatgg cgcggttaca
    18541 gccgctcgct ttaatacctt tacgtacaac tttaaaaatt tcgtgcagcg cctcgtattc
    18601 gctgaccgcg ccgcgatcgg gatcgttcgg gtcaatgccg ttgaaggcga gggcttctgg
    18661 ttgcaaattt gcgccgacaa atggttcgac gtggaaatgt aatgtggtgt ccggcatcag
    18721 ccagccttgt tcatccattt tcagggtgat ggcggcaatc tcaagcagcg catcggtttt
    18781 ggcgttaaat ccggctgttt caacatcgat cacaacagga taaaaaccac gaaaacggtc
    18841 gcacagaccg gtaagttgag cgttatcgga catctgattc tcttaatacg gataaattgc
    18901 agcgcgcatt atgacaaata ttacttgtcg atgcagtaaa gatgcgggcg cgatgagttc
    18961 acgcccggca ggagattagt tgcccagacc gcgaccggcg tctttttctt cgattaactc
    19021 aattttgtaa ccgtccggat cttccacaaa cgcgataacc gtagtaccgc cttttaccgg
    19081 acccgcttca cgggtcacgt tacccccgtt ttgacggatt ttttcgcacg cttcagcggc
    19141 gttatctacg ctaagcgcga tgtgaccaaa agcagtgccg agttcgtatt tatccacgcc
    19201 ccagttgtag gtcagttcaa tcaccgcttc ttcggtttcc gggccgtagc caacaaacgc
    19261 cagtgagtat ttgtattccg ggttttcgct ggtacgcagc agtttcatgc ccagcacttt
    19321 ggtataaaaa tcgatggagc gttgcaaatc gccaacgcgc agcatggtat gaagaagacg
    19381 catcttttta tcctcaaaat ggctaacaga ttcaaaatgt tgttttagta tagcggcggc
    19441 tttacgccgc tcgcaatgtt ggattacaac gtcgggtaat cggtatagcc ttccgcgccg
    19501 ccaccgtaga aactttcggc acgctgtggg ttaagctcag ctttgcgctg caagcgggcg
    19561 accagatccg ggttcgcaat ccagtcacga ccaaatgcca ccgcatcaat taaccctttg
    19621 ccgatcagcg tttcagcttt ttctactgtg tatgcacctg cgccgataat cggaccgtgg
    19681 aaacgggcgc gtactttttc gcggaacgca tcagtatacg gttcaccccc cgcccaatct
    19741 ggttctgaca tatgcagata agcaatgccg cgtttaccca gttgttcaat cagatacagt
    19801 gcatcggctt cttcatccgg gccgttatcc gtgttctgga aagtaccgat tggcgaaatg
    19861 cgaatgccaa tgcgatcggc accccattct tcaatcccgg catcgaccac ttccagtacc
    19921 agacgtgcgc gattttccac gctgccgccg tactgatcgg tacgatggtt tgaagaagga
    19981 gaaaggaact gatgcagcaa ataaccgtga gcagagtgga gctctaccag atcaaaaccg
    20041 gcttcacgcg cgttagcaat ggcctgacgg aaatcattga cgatacctgg aatctcttcc
    20101 agttcaagcg cacgcggcat ggatgtttca acacggatcg cctgaccatt ttcatcgcgc
    20161 agagaagtac gtgttcccgc gctaagtgct gaaggcgcta ccggtgcctg accgccaggt
    20221 tgcaggctgg cgtgagaaat gcgtccggtg tgccacagct gcacggccat atgaccattt
    20281 tcagcatgaa cgccagcggt gatttttttc catgcggcaa tttgctccgg actatggatg
    20341 ccaggcgcac ctgcatatcc ttttgcctgg gcagaaattt gcgtggcttc actaataatc
    20401 aaaccggcac tggcacgttg gcgatagtat tccgccatca acggggtagg aatgtcaccc
    20461 ggttcaatac tgcgcagacg cgtcagcggt gccataaaaa tacggtttgc cgccgtgatc
    20521 gcgcccactt tcagtgggga atacagtttt tcagatgaca taaaggctcc cggtcgtctg
    20581 gtgataaaaa atgcctgcta aacggcaggc gtcgcaataa tgttttttac atgggccagc
    20641 gcgttttcca gtggctcgaa actgcgcgaa attttggcct gcagattcgc gccaagccac
    20701 agtgcgtaaa gcacttgtgc ctgttgcagc ggttcgccac aaaaggttaa acaatggttc
    20761 tcacggccat tttccagcgc ctgagagagc agggcgatca cgccgcgagc acctttatcc
    20821 atcgcgctgc gcatatcttc tgacagatcg cacacttcgg cagagagttt tactgtcagg
    20881 caaccactga tggttccatg ttggcaaaac tggttcagtg tttgctggta ataagccagt
    20941 atgcggtcgc ggtagttacc ttcgccggat tgcagcaact cagtcagtcg ctggtgatat
    21001 gcggcgtaat gacgctcaag catggcaacg ccaaacgctt ctttagagcg aaagtagtga
    21061 tagaaggacc ctttcggcac ttcagcggtt tttagtaatt cgcttagccc catcccggtg
    21121 aatccacgtt gcaggcaaag ttgctcgccc gtcgccagga gatgttcgcg agtatcatgt
    21181 tcggtgtgtt tgttcatgcg ttggagtgta gtagaccagt cggtctagtg caagcaaagt
    21241 tgctactttg gacattaaca gcttcaataa tggtaataat cttatctgag gagggcgaag
    21301 tggcggagca attagagttc tttcccgtcc agagcccgtg ccggggaatt tgccagtctg
    21361 acgaacgcgg gttttgtcgc ggctgttttc gtagtcgtga tgagcgtttt aactggaata
    21421 aaatgagcga tggcgaaaaa caagaggtgc tgcgcctttg tcggcagcga ttaatgcgca
    21481 aattacgcgc aaataagcca gcgtcatcag acgaaccgga gcaaccatca ctcttttgaa
    21541 cctaatcttg cgtatactcc tctcatatct gataagagga agcgattatg gttcagcgta
    21601 ttactattgc gccgcaaggc ccggagtttt cccgttttgt gatgggctac tggcgattga
    21661 tggactggaa tatgtccgcc cgccagctgg tcagttttat tgaagagcat ctggatctcg
    21721 gcgtgaccac cgtggaccat gctgatattt atggtggcta tcagtgcgaa gcggcgtttg
    21781 gcgaggcact gaaactggca cctcacctgc gtgaacggat ggaaatcgtc agtaaatgcg
    21841 gtatcgcgac gaccgcgcgt gaagaaaacg tcattggtca ttacatcact gaccgcgatc
    21901 acatcattaa gagcgccgaa cagtcgctaa ttaatctcgc gaccgatcat ctggatctgc
    21961 tgttaatcca ccgaccagac ccgttaatgg atgccgatga agtggcggac gcgttcaaac
    22021 atctgcatca gagcggcaaa gtgcgtcatt ttggcgtatc gaactttacg cctgcgcaat
    22081 ttgccctgtt gcaatcacgt ctgccgttta cccttgccac taatcaggtg gaaatatccc
    22141 cggtgcatca gccgttactg ctggatggca cgctcgacca actacaacaa ctgcgtgttc
    22201 gtccgatggc gtggtcctgc cttggtggtg gtcgtctgtt taatgatgat tatttccagc
    22261 cgctgcgtga tgaactggct gtggtggcag aggagttaaa cgcgggctcg attgaacagg
    22321 tggtttacgc ctgggtatta cgtttaccat cgcagccgct gccaattatc ggttcaggta
    22381 aaattgagcg cgtacgggca gctgtcgaag cagaaacact gaaaatgacc cgtcaacaat
    22441 ggtttcgtat ccgtaaagcg gcactggggt acgacgtacc gtaagcactt ttaggaatag
    22501 ccgccgttca aaaatgtgtc actggtttac acttattcag gaatgcacaa tgaacggagg
    22561 tcctatgaaa cgttttagtc tggctattct ggcgctggtt gttgcaaccg gcgcacaagc
    22621 tgccagtgaa aaagtcgaga tgaacctcgt cacgtcgcaa ggggtagggc agtcaattgg
    22681 tagcgtcacc attactgaaa ccgataaagg tctggagttt tcgcccgatc tgaaagcatt
    22741 accccccggt gaacatggct tccatattca tgccaaagga agctgccagc cagccaccaa
    22801 agatggcaaa gccagcgccg cggaatccgc aggcgggcat cttgatccac aaaataccgg
    22861 taaacatgaa gggccagaag gtgccgggca tttaggcgat ctgcctgcac tggtcgtcaa
    22921 taatgacggc aaagctaccg atgccgtcat cgcgcctcgt ctgaaatcac tggatgaaat
    22981 caaagacaaa gcgctgatgg tccacgttgg cggcgataat atgtccgatc aacctaaacc
    23041 gctgggcggt ggcggtgaac gctatgcctg tggtgtaatt aagtaattaa gaggccagcg
    23101 taccttgcgg tggtgcttgc tcaagttgcg aaagcgagca gtacagccgc cagacaattg
    23161 ccgccagctc acgggcggca ggttgatgat gacgggcaag gctgtcgcaa atccgctgta
    23221 attcttcaag tgtggccgcc agtgattttt gctgaacgcc acgctcactc atcactcccc
    23281 gcaacagtga aatacagtta tcccgtactc gcgataacgg atcggaacgc gattcccagt
    23341 cgcgcaattg ccagacaaca tgagaacagt tcagcagcac tacaccccag cgtaataacc
    23401 aacggcgagc cagcgcatcc tggctgttac tcaactgact gacgtgatga taagtgagcg
    23461 attcaaattc gctttcactc agtgttggat ggcggcttag ctgatcgaca aaatcccggc
    23521 gcagcgcgcg aatatggcgg cggcttttac gagcatccga tcctggacgc agaatggcga
    23581 acgctaacca cgccaacgcg acgccaacga tttttgccag attatcgtta agaaaatcag
    23641 caaaatcata caccggggga ttagtgacag cgataaaaga acccataaaa acaatcagtt
    23701 gcccccacaa tgcggcaaat tttggcatct gcaatttaag aagctgcatt gtcgccagca
    23761 gtggaaagag aaacagtaaa aattgccaca gatcgctaat ctggaccatc agaccaaatt
    23821 tgaccacaaa gctgaatagc gaaagtaaca ccagcgtgcg catcagaagt gacaacgact
    23881 taaacggtgc tgcgacggcg gagtagagta cgcagctaat tgctgccagc gttaatgcat
    23941 tggcaccggc atcccattgc gatgcaatac tccatgcgcc aatcatcatc aacgtacaaa
    24001 atgtacgcag cccgctccac atagcttcgg cgttgtcggt atgacgagcc agagcggatg
    24061 tccgtgcgag tctggcatga tcatctacac cgctttgcag acgatgtaat tcctgactac
    24121 tttgcagata aaggcggcaa aaataacgta gtcgctgcca gaaggcgacg tgccggtagt
    24181 cggcgacgtt ggtcgggcgt agcggggcga taatacgtgc gacggtgtaa gcatctgttt
    24241 gcgaactggc gagcgtcgtc agcaattgtt cgagaatttc tcgcgtggca cctggcggtg
    24301 agggccagtt gagcaacata cgtcgcaggc tggagatgac actggtcata cgtaattgct
    24361 ggtggagtag cgcattaagg cgcgcgtttt gctggcgaaa acgatagtgg ctccagaaag
    24421 cctggatacg cagcaaattc atggtcagta tctgcccaat caccccctca tgtgctgcac
    24481 gaatggcatc gtttgtttca ggctgccaga gtaaactggc atgttccagt aatcgggcgt
    24541 gcatgttttt caatgcggtt aaaagggctg tagcatcgga actgctaggc aggatcatca
    24601 tcatcatgcc gccgcacaaa ataccgacta tcacctcgca aacgcgcgcc tgagcgatat
    24661 cccacagctg gctggcctca gtaatattaa ccatcggaaa ggcgatgatg gcagccgtgt
    24721 agcccgccag ttgaaatgca tacgcgacgt tattcgtgaa gtgcgcacag gcccaggtac
    24781 aaaagccaag ccacgccgac atgctcaata gaaaaaacca cggctcattg agcgtatgcc
    24841 cggcaagaag cagtgccgca atggctccca gtaaactgcc agcgatgcgt ccgaggcttt
    24901 tgctgatgac accgccaacg gtgggaaagc taaccactgc agccgaggtc atcgcccaat
    24961 agggttcatc cagattcaaa taataggcaa ccgtcagcgc cagacacatg gcaatggtat
    25021 tgcgtaacgc ataacgccat tgcgccagcg tggccttgaa ccagggcaaa ttgcgcaagg
    25081 accatgacga tgcgttcatt attgacctac ggcaatgcta caggtggtgc ccgacaccag
    25141 cgtgacatcg cctggaactt tatccagcgc aaagcggacg ggaacgcgtt gggcgagacg
    25201 aacccagggg acgttaggtt tcacatccgg aataaggctt gagtcacttt caacgctttg
    25261 atcataaatc gcccgaccga tactggaaac gtgaccccgt aacgttttgt tatcactata
    25321 gagggtaatt tgtgctggtg caccttcgcg gatatggcgc aacttggttt cttcaaaata
    25381 accaatgacg taaaacgagt ggctgtcgac aagggcaaac agcggtttcc cggtgtcagc
    25441 gtaatcgccg atgcgggtag tgaggttagt cacccaacca ctcaccgggg cgcgaatttc
    25501 cgtttgcgcc agttgccatt gtgtctgttt cagcgtggcc tgtgcggcat taacactggc
    25561 ctgcatcgct ttaacattga ggttagcggt atcgagttct tcggcagaaa taaaattttg
    25621 tgagagatga cgacgacgat tcgcttcgtt attggctttt gcgaggtcag attgcgcttt
    25681 cgccagctga gcctgtgcgt tcagctcggc gatctgaaag ggcgttttat cgatcgtgag
    25741 cagaagatcc cctgcgttaa ccagctggtt gtcttttata ttcagctcaa caatacgtcc
    25801 cgacacctgt ggggtgatag aaacctgttc tgcgcgtatt ttcccgtcgc gtgtccacgg
    25861 agattgcatg taataattcc acagccacca tccggcaaga accgcaacta ccgctacaat
    25921 gattgttgag aaatacttaa ttgttttaat tgacatagtt accacgcaat cagtattgcc
    25981 agagcaaggc aaacgcaaat cgcaaacagc gataaatcca ttaacaaggg atgccagatg
    26041 tcaccggcgt agatccagcc acgaagcagg cggtgtacga caagccagat gacgaatcca
    26101 aacgcgaagg ctttgaaaaa cggaggaaag tagacggacg caccgaacac cagatcttgt
    26161 aagggcaatc ctgttgcatt gagcataaac ttcacaaagt agattccttt acgaccgaat
    26221 atattgcgtt agattaataa atattcttta agtgcgaaaa atttacgcgc aatttctgaa
    26281 aaatatgtct gacggtaacc aaatgcagca atacatttgt tttagcaata caattgctgc
    26341 acactattct aaaagccgca taatatctta gcaagctaat tataaggaga tgaaattgga
    26401 atcgccacta ggttctgatc tggcacggtt ggtgcgcata tggcgtgctc tgatagacca
    26461 tcgcctgaaa ccgctggagt taacacaaac ccattgggtt acgttacaca atatccatca
    26521 gttacctcca gaccagtcgc aaattcaact ggcaaaagcg attggcatcg agcagccatc
    26581 actggtccgt actctggacc aactggaaga aaaagggtta atttcgcgtc aaacttgtgc
    26641 cagcgatcgt cgggctaaac gtattaaact gacggaaaag gcagagccac tgatcagcga
    26701 aatggaagct gttattaaca aaacccgcgc ggaaatatta catggcatct ccgcagagga
    26761 actggaacaa ctgattacgc tcatcgcaaa acttgagcat aatatcattg agttacaggc
    26821 caaagggtga aatgaagggg gcgtgtggtc agtaacctga ccacacgcaa acttatttag
    26881 cgcggagaaa cggtcacctg actgccattg ctggccagta cgacacgttg gcccggagag
    26941 aaacgagtgt tgccttgttt ctgtaccacc atgatggtat taccatcgtc tttacgaatt
    27001 tccagctcga caccctgcgt tttgttcatt gcactctgta cgccctgacc agctacgcca
    27061 cctgcaacag cgcctgctgc agtagccaga gaacgcccgg ttccgccacc aacagtattc
    27121 cccaggaaac caccaagaac agcaccgcca attgcaccga taacgttgga atcatcaccg
    27181 ccctgaatct gtaccggacg tacgttaacg atggtgccat agctgacatt ctgtacttgt
    27241 ttcgcttcag aagcggtata aacatcccct gacagggtgt cgttattaac acaaccgaca
    27301 agagacagac ctaccattga aacaaccaat acgcgtttaa tcattgaaaa atctcctgtt
    27361 caccatgaaa cgccacgcaa gcatccctca tggtcaaagt atatggcata tgtgaatcca
    27421 tggttacatg ttctaccaaa tatgagcaaa tcataatgaa agtaggatca accaattata
    27481 aacaaaacat tcatgatgat tattgatttg aagataatgt tttactacca acggtaaaaa
    27541 tcggtcattc cgcatggtat tcacgaaaag tttatgtttg agtggtgcgc aaacgtcaga
    27601 ttgaaaaagg atgaactatg aaatcgggcc gctttattgg cgttatgtca ggcaccagcc
    27661 ttgatggtgt tgatgttgtg ttggcgacaa tagatgaaca ccgggtcgca cagctggcaa
    27721 gcttgagctg gccgatcccg gtgtctctga aacaggctgt actggatatt tgccagggcc
    27781 agcagcttac actttcgcag tttggacagc ttgatactca actcgggcga ctttttgctg
    27841 atgcggtcaa tgccttgctt aaggaacaaa acctgcaggc gagagatata gttgcgatcg
    27901 gctgtcacgg tcaaaccgtc tggcatgaac cgacgggcgt ggcaccacac actttacaga
    27961 ttggcgataa caatcaaatt gtggcacgca ccggaattac ggttgtcggt gattttcgcc
    28021 gtcgcgatat tgccttggga ggacaaggcg caccgctggt acctgcgttc catcatgcct
    28081 tgctggctca cccaaccgag cgacgaatgg tgctcaatat tggcggcatc gccaatctgt
    28141 cactgctcat tcctgggcag ccggttggcg gctacgatac cggtcctggt aacatgctga
    28201 tggatgcctg gatctggcgt caggccggta aaccttacga taaagatgcc gagtgggcac
    28261 gggcgggtaa agttattctc ccactgctgc aaaatatgct cagcgaccca tatttctcgc
    28321 aacctgcacc gaaaagcacc ggacgcgaat actttaacta cggctggctg gagcgccatt
    28381 tacgccattt tccgggtgtt gatccccgtg atgttcaggc cacactggca gaactcaccg
    28441 ccgtgaccat ttctgaacaa gttttgttga gcggtggctg cgaacgattg atggtatgtg
    28501 gtggaggtag ttgtaatccg ctactcatgg cgcgtctggc ggcattactg ccaggcacag
    28561 aagtcaccac caccgatgcc gttggcatta gtggcgatga catggaagca ttggctttcg
    28621 cctggcttgc ctggcggacg ctggcgggat taccaggaaa tctgccttcc gtcactggcg
    28681 caagccagga gacggtactg ggggctattt tccctgctaa cccgtgatat cagagttaac
    28741 tgaatttttc ttaccgcgtc tgccgttaca cttggaacgt tagggagggc gtattgccct
    28801 ccagaccagg aaagtcttcg ggaaatgtct atgaaaaaac tgttactgat ctgtttgccg
    28861 gtgcttctca ccggctgtag cgcgttcaat caactggttg agcacatgca aaccgctacg
    28921 ctggaatacc agtgtgatga aaaaccgttg acggtcaaac tgaataatcc gcgccaggaa
    28981 gtcagttttg tttacgataa tcaactactg catctcaaac agggcatttc agcgtctggt
    29041 gcacgttaca ctgacggaat ctatgttttc tggtcgaaag gcgatgaagc gactgtctat
    29101 aaacgcgacc gcatcgtatt gaataactgt cagttacaaa atccacagcg ttgagatttt
    29161 tccaggggcg gcgcacaata gcgtcaccca ctgacaatcc gtaaagaaaa ccatgtctga
    29221 taacgacgaa ttgcagcaaa tcgcgcatct gcgccgtgaa tacaccaaag gcgggttacg
    29281 ccgtcgcgat ctccccgccg atccattaac cctttttgaa cgttggctct ctcaggcgtg
    29341 tgaagccaaa ctggcagacc ctaccgcgat ggtggtcgct accgtggatg aacatgctca
    29401 gccttatcaa cgcatcgttt tactcaaaca ttacgacgaa aaaggcatgg tgttttacac
    29461 caacctcggt agccgtaaag cacatcaaat tgaaaataat ccgcgcgtta gcctgctgtt
    29521 cccgtggcat acccttgaac gccaggtgat ggtgatcggt aaagcagaac gactttcgac
    29581 tctcgaagtg atgaaatatt ttcatagccg cccgcgtgat agccagattg gtgcatgggt
    29641 ttcgaagcag tccagtcgca tttctgcccg cggtatcctt gaaagtaaat tcctggagct
    29701 gaagcagaag tttcaacagg gcgaagtgcc attgccgagc ttttggggcg gttttcgcgt
    29761 cagccttgaa cagattgagt tctggcaggg cggtgagcat cgcctgcatg accgcttttt
    29821 gtaccagcga gaaaatgatg cgtggaagat tgatcgtctt gcaccctgaa aagatgcaaa
    29881 aatcttgctt taatcgctgg tactcctgat tctggcactt tattctatgt ctctttcgca
    29941 tctggcgaaa agtcgtgtac cggcaaaggt gcagtcgtta tatacatgga gattttgatg
    30001 gcaagcagta acttgattaa acaattgcaa gagcgggggc tggtagccca ggtgacggac
    30061 gaggaagcgt tagcagagcg actggcgcaa ggcccgatcg cgctctattg cggcttcgat
    30121 cctaccgctg acagcttgca tttggggcat cttgttccat tgttatgcct gaaacgcttc
    30181 cagcaggcgg gccacaagcc ggttgcgctg gtaggcggcg cgacgggtct gattggcgac
    30241 ccgagcttca aagctgccga gcgtaagctg aacaccgaag aaactgttca ggagtgggtg
    30301 gacaaaatcc gtaagcaggt tgccccgttc ctcgatttcg actgtggaga aaactctgct
    30361 atcgcggcga acaactatga ctggttcggc aatatgaatg tgctgacctt cctgcgcgat
    30421 attggcaaac acttctccgt taaccagatg atcaacaaag aagcggttaa gcagcgtctc
    30481 aaccgtgaag atcaggggat ttcgttcact gagttttcct acaacctgtt gcagggttat
    30541 gacttcgcct gtctgaacaa acagtacggt gtggtgctgc aaattggtgg ttctgaccag
    30601 tggggtaaca tcacttctgg tatcgacctg acccgtcgtc tgcatcagaa tcaggtgttt
    30661 ggcctgaccg ttccgctgat cactaaagca gatggcacca aatttggtaa aactgaaggc
    30721 ggcgcagtct ggttggatcc gaagaaaacc agcccgtaca aattctacca gttctggatc
    30781 aacactgcgg atgccgacgt ttaccgcttc ctgaagttct tcacctttat gagcattgaa
    30841 gagatcaacg ccctggaaga agaagataaa aacagcggta aagcaccgcg cgcccagtat
    30901 gtactggcgg agcaggtgac tcgtctggtt cacggtgaag aaggtttaca ggcggcaaaa
    30961 cgtattaccg aatgcctgtt cagcggttct ttgagtgcgc tgagtgaagc ggacttcgaa
    31021 cagctggcgc aggacggcgt accgatggtt gagatggaaa agggcgcaga cctgatgcag
    31081 gcgctggtcg attctgaact gcaaccttcc cgtggtcagg cgcgtaaaac tatcgcctcc
    31141 aatgccatca ccattaacgg tgaaaaacag tccgatcctg aatacttctt taaagaagaa
    31201 gatcgtctgt ttggtcgttt taccttactg cgtcgcggca aaaagaatta ctgtctgatt
    31261 tgctggaaat aatgcattaa gtggaaaggg ggagtgagaa atcactcccc ctggttttta
    31321 tacagggaac atgatgaaaa atattctcgc tatccagtct cacgttgttt atggtcatgc
    31381 gggtaacagt gcggcagagt ttcctatgcg ccgcctgggc gcgaacgtct ggccgctgaa
    31441 caccgttcaa ttttctaatc acacccaata cggcaaatgg actggctgcg tgatgccgcc
    31501 cagccattta accgaaattg tgcaaggcat tgccgccatt gataaattac acacctgtga
    31561 tgccgtgtta agtggctatc tgggatcggc ggagcagggt gaacatatcc tcggtatcgt
    31621 ccgtcaggtg aaagccgcga atccgcaggc gaaatatttt tgcgatccgg taatgggtca
    31681 tccggaaaaa ggctgtatcg ttgcaccggg tgtcgcagag tttcatgtgc ggcacggttt
    31741 gcctgccagc gatatcattg cgccgaatct ggttgagctg gaaatactct gtgagcatgc
    31801 ggtaaataac gtcgaagaag cggttctggc agcgcgcgaa ctcattgcgc aagggccaca
    31861 aattgtgttg gttaaacacc tggcgcgagc tggctacagc cgtgaccgtt ttgaaatgct
    31921 gctggtcacc gccgatgaag cctggcatat cagccgtccg ctggtggatt ttggtatgcg
    31981 ccagccggta ggtgttggtg atgtgacgag cggtttactg ctggtgaaac tgcttcaggg
    32041 ggcaacgctg caggaggcgc tggaacatgt gaccgctgca gtctacgaaa tcatggtgac
    32101 caccaaagca atgcaggaat atgagctgca agtggtggct gctcaggatc gtattgccaa
    32161 accagaacat tacttcagcg caacaaagct ctgaaatatc tcatttcggc ccacaacgac
    32221 ctgtgggccg atggatatta ctttaagcct tccgctgaca gcgcgtcttg tacttccgga
    32281 cgttcagcca tacgttgcat aaatgctgca atgtgctcta acccttccag attcagtttc
    32341 accgcgtatg cccagcgcag aaccgtaaac agataggcat cagcaattgt aaatctttgc
    32401 ccgcagatcc agtgctcatc cttcagtgcc tcgttcacat attgcagctt cttctccagc
    32461 tgcgcgcgaa ctgtcggttt gtactcttcc ggtgtatcag ggcgaaacag aggtgtgaaa
    32521 cctttatgca gctcggtggc gatgtaattc agccattcga tggttttata gcgggaaata
    32581 ctgtttaccg gtgccagcaa ctggcggtcg gggacgctgt cggcaagata ctgcataatc
    32641 gctacgcctt ccgtcagcaa agtaccgtca tccagcagca atgcaggcac ctgcccctta
    32701 gggttaacgg caaagtaatc gtcaccgttt tcgagacgtt ttttcattaa atccacactg
    32761 acgagggtaa aatcctttcc gctctcacgc agggtgatat gggaagcgag agagcaggca
    32821 cccggtttgt agaacaattt catcggtaac tccttttgtc tgtggtttaa agtatggtag
    32881 tgcgttgctg aacaaaaaaa aagccgctaa cgaaaagtta gcggcttgta aaagagtttc
    32941 cctgaatttt acgctacagc agctttcgcc gctttgtctg cagcgtcatc ctgcgtcata
    33001 cggtgcagtt taggcgcggt cagcagcatc agtactgcaa taatggcagt agcgacacca
    33061 atctgcaaga atacgcgacc ataaacttcc agtgacatca gcgggtcggt aacgttatcc
    33121 ggcacagcca tcatacccgc aacataacca ccaatcaggt ttgcaccggc agtggtcagg
    33181 aaccagctac ccataatgaa gcccatcaga cgctgcggaa cgagttgtgc aaccattgcc
    33241 agacccagac cagagatcat cagttccccg atgctctgca ggccatagct tgcgaccagc
    33301 cagcttacag acacgatacc agcgtcagac gcgaatttcg cacccagcgg cagaatcagg
    33361 aacgcaccag aacacatcac catgccgatt gcaaacttgg ttggcatcgg cagggtatcg
    33421 cccatcttgt tatagatagc ggccagaatc ggactaccga tgatgatcca gaacgggttc
    33481 agtgcctgat actgctcagg ttctacggcc agacccagaa tggagtgctc aacgttacga
    33541 atcgcaaaga agttcagtga cgttggcatc tggctgtaca gcacgaagaa gataatggct
    33601 tcgagcatca ggatgaaggc aacgatcatt ttacgacgcg cagcaccttt catcgcgaag
    33661 gcttctttac cgaagataac cacgataccg aaggcaacaa cgcccagcgc catacgcgca
    33721 acttcctgat tgtgcagcag ccaggtggcg atagcgatca gtgccacaac accaataatg
    33781 gtcagcagca ggttacggta gttgataggc tcgaagtctg gttttgaacc gtactgttta
    33841 acccagcgtt ggcagaaggc gaagttaacg atagtgatca gcaggcctac aacgctcaac
    33901 gcaaacgcaa cactccagcc gtatttcgcg gccagccacg gcgtagcaat catagagaag
    33961 aaagagccga tgttgacgga catgtagtac atggtgaatg caccgtccag acgcgggtcg
    34021 tttttctcat agcatgtaga aagcagagaa gacgggttag ctttaaacag gccgttaccg
    34081 accgcaatag ccgccatacc catataaacg ataccggcgt cgtgaccaga ccaggcaacc
    34141 agagcataac caatcgccag cacaatagcg ccgagcataa ttacgcgttt agtacccagt
    34201 accttgtcac ctaaccagcc gccgatagcg accagaccat aaaccagggc actaaaggaa
    34261 gagaaaaggg tgattgaatc cgcttcagac atacccagtt gtttaaccag gtaaacagcc
    34321 ataattcctt gtaggccgta ataaccaaaa cgttcccata actcaatcga gaagatgaga
    34381 tagaacgcct tcggttgttt gaaagcgttc aaactgacgc tttcagttgg tttttggttt
    34441 gcagtggaca cttttacctc tttttttaca tcccatatta acgggggtgc tttctcgcgt
    34501 tatgtctaaa aaacatacgc gcaattgtta tagtgggagg ggaaacggca ggtaatgttc
    34561 actatcttgc actttcagac aagtcctttg taataatctg ttacatataa ctgaggcggg
    34621 gaattcaacc attccgggaa atgttatcca gcgttaaatt tttcaaaaaa cgtaacggca
    34681 gattttttca ctgcactgag aggggggtga tggcttaacg gcgatatgtt ctgctattta
    34741 gcgtttttac tagtgagata gtccggtttc tgaaaaatag ccagtgtaat gttttgtagg
    34801 tcaatatttg ttgcgtgtgt aaatttaaat gattttaacc tgattcttgc aatgttttta
    34861 cattgcgaca gcatcgtgat cttgatcacg aatgaataga aaatttcgct attaaaaaaa
    34921 caattaacct gcatttttgg ttatccgcaa acggattacc tgatgataac gctaagcaat
    34981 ggcatcattc acgttaaaaa gatgttttaa aagaggataa agaaaggtta tcaatggggt
    35041 aatcggtgtt accccttttc ttcagatgtc aactttctct ttgtattcac aaagatcttc
    35101 aataatacaa gagccacagc ggggcttgcg ggcaatgcag gtataacgcc cgtgcaggat
    35161 caaccaatgg tggcagtcga ctttaaactc tgccggaacc actttcagta gcttttcttc
    35221 tacctgttcg acgtttttcc ccggcgcaaa ttgagtacga ttacaaacgc ggaaaatgtg
    35281 cgtgtcgaca gcaatagtcg gccagccgaa tgcagtgttt aatacgacgt tggctgtttt
    35341 acgacctacg ccgggcaggg cttcaagcgc agcacgatct tccggaacct cgccattatg
    35401 ctgctccagt aagatacggc aggttttgat gatattttct gctttgctgt tataaagccc
    35461 aatcgttttg atataggttt taaccccttc aacgcccagt tcaagcatcg ctgcaggcgt
    35521 attcgccacc gggtaaagtt tcgccgtcgc cttattaaca ctgacatcgg tcgcctgagc
    35581 ggaaagcagt acggcaatca gcaattcaaa aggcgaactg aaattaagct cggtggtggg
    35641 atgaggattg ttctcacgca ggcgagtgag gatctccagg cgttttgctt tgttcatcag
    35701 acattccctg tttcaccgtt tggcaatgca cgttcggcag ctgcttcagt acggcgcttt
    35761 ttcattcttt catcaatcag gtattttcct gccagcatca gtcccaggcc aataaatgca
    35821 cctggtggca gcatcgccag caggaaaggg gagtcggtgt ggaaaatctc tacgcgtaat
    35881 acttttgccc agctacctaa cagcgcatct gcaccgtcaa acaatgtgcc attgccgata
    35941 atttcgcgta gtgaacccag cacgaacatg gcgcaggttg cgcccatacc aattgaaaag
    36001 ccgtccagtg ccgaaagcgc cggacctttt ttggcggcga aggcttcagc gcggcccaca
    36061 acgatacagt tagtgacaat cagcggaata aaaatcccta atgattgata caggccaaag
    36121 gcgtaggcgt tgattagcat ctgtacagcg ctgaccaccg aggcgatgat catcacgtaa
    36181 atgggaatgc ggatctcggc tggcgtccag tgacgcagcg tcgaaatggt caggttggtc
    36241 agcgtcaata ccagcgtagt cgcaagtcct aaacccagag cgttagtggc agtggacgtg
    36301 accgccaaca gaggacaaag gccgagcaac tggaccagcg cagagttgtt tttccacaac
    36361 ccctgaacaa taacgtcttt aatttcgctc acgctttatt ctccacaggc aggaagttga
    36421 gaaagttgtg ccggtaacgt ctgagcgtac aatccggcgc gttttaccgc attaaccacc
    36481 gcgcggggag taatcgtcgc gccggtgaac tggtcgaaat caccaccatc tttcttcacc
    36541 gcccagtgcg catcatccgc accactgatt tttttacccg caaaatgggt gatccagtca
    36601 gaaaggcgca gttcgatttt atcgccaagc cctggcgttt cgtggtgctc tgtcacgcgc
    36661 gtgccaagta ccgtgccgtt aaaatcggct cccaccagca gctgaatcgc accggaatag
    36721 ccatctggcg cggttgcttc cagaacggcg gctaccggtt tgtcatcctg tttggcgatg
    36781 taaacccgat gctcaccttt acctaactct ggcgcagtta ccagatagca actctgtgcc
    36841 agcgcattgt tatagcgttc ggctggcagc acctgatcaa ataatgcctt ttgttgcaga
    36901 ctggcctgtt cagcaatcgt cgttttggtc atctggttga tggccgcagt taaccctgtt
    36961 gaacccgctg caaatagcgc taacgtaatg ccgtgttttc ggatagtttt cagcatggtt
    37021 taccctttgc gatggccgta gacgcgcgga cgcgtgtagt aatcgatcag aggaaccgtg
    37081 atgttcgcca gcaggacggc aaaagccacg ccgtcaggat agccgccgaa actgcggatc
    37141 aaccagacta ataagcccgc aagcgcgccg aaaataagac gaccacgatt ggtcgtagaa
    37201 gcggtaaccg ggtcagtcaa aataaagaat gcgccgagca tggtcgcacc agacagcaga
    37261 tgaatttgcg gtgctgccag tgtttctggt gagaacaacc agcccaacgt tgcgcataac
    37321 gccagcgtta ctaagaagct gaggggaata tgccagcgaa tcgctttctg ccatagcaac
    37381 cacaggccgc cagccagcca ggcgagattt acccattgcc aaccagcgcc cgccagaata
    37441 ccgctgtaga tcggatattg cataatctgt tcaaccgaat gaccggcacg gacagaggtt
    37501 ttaaatgtat ccagcggtgt cgcctgacta atgccatcaa tacctaagcg tagtgtgttc
    37561 atatcaccac cactggcggt atgcccgctg aaaataacct gtatggcgtc gataaaacca
    37621 gggatgttga ccgcaatttc atgtggcggt aaccagctgg tcatctgcac cgggaaggag
    37681 atcagtaaga ccacataacc aatcattgcc ggattaaacg ggttttgccc cagaccgcca
    37741 tacaactgtt tagcgatgat caccgcaaac accgtaccca gcacgaccat ccaccatggc
    37801 gcgagggggg gaatacttac cgccagcaat aagcctgtca gcaatgctga gttatctttc
    37861 aacgttgcgg ctaccgactg cttgcgtagt ttgagtacga gagcttcggc taacagagca
    37921 ctaaccgatg ccaacaggat ctgaacgaga gtaccccaac caaaaaacca cagttgcgct
    37981 gcgattcctg gcacggctgc gagcaacacc aacagcataa tgcgcgatgt ctggcgctgg
    38041 ttatgggtat aaggggagct agctattctg aataccattt agtcctcgtt tacaaccttc
    38101 tgctgggcgg cttttttggc ctgagcgcgg gcaatagccg cggcaactgc cgctttgcgc
    38161 ggatcaacct gttcttctgg ttctgcgtta gtctgttgct gttccagc
//
LOCUS       DAOFQD010000033        37769 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.033, whole genome shotgun sequence.
ACCESSION   DAOFQD010000033 DAOFQD010000000
VERSION     DAOFQD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.033"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            336..1709
                     /gene="aroP"
                     /locus_tag="QBF46_001753"
     CDS             336..1709
                     /gene="aroP"
                     /locus_tag="QBF46_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDQ6673942.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1752..2606)
                     /gene="ampE"
                     /locus_tag="QBF46_001754"
     CDS             complement(1752..2606)
                     /gene="ampE"
                     /locus_tag="QBF46_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDQ6673943.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2603..3154)
                     /gene="ampD"
                     /locus_tag="QBF46_001755"
     CDS             complement(2603..3154)
                     /gene="ampD"
                     /locus_tag="QBF46_001755"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDQ6673944.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3242..4135
                     /gene="nadC"
                     /locus_tag="QBF46_001756"
     CDS             3242..4135
                     /gene="nadC"
                     /locus_tag="QBF46_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDQ6673945.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINTNQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4338..4778
                     /gene="ppdD"
                     /locus_tag="QBF46_001757"
     CDS             4338..4778
                     /gene="ppdD"
                     /locus_tag="QBF46_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDQ6673946.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN"
     gene            4788..6173
                     /gene="gspE"
                     /locus_tag="QBF46_001758"
     CDS             4788..6173
                     /gene="gspE"
                     /locus_tag="QBF46_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDQ6673947.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARHAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6163..7365
                     /gene="hofC"
                     /locus_tag="QBF46_001759"
     CDS             6163..7365
                     /gene="hofC"
                     /locus_tag="QBF46_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDQ6673948.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMRWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYRAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRENTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7400..8443)
                     /gene="guaC"
                     /locus_tag="QBF46_001760"
     CDS             complement(7400..8443)
                     /gene="guaC"
                     /locus_tag="QBF46_001760"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDQ6673949.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8599..8643)
                     /locus_tag="QBF46_001761"
     CDS             complement(8599..8643)
                     /locus_tag="QBF46_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673950.1"
                     /translation="MIIKTLIMSARKRN"
     gene            8668..9288
                     /gene="coaE"
                     /locus_tag="QBF46_001762"
     CDS             8668..9288
                     /gene="coaE"
                     /locus_tag="QBF46_001762"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDQ6673951.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9288..10031
                     /gene="zapD"
                     /locus_tag="QBF46_001763"
     CDS             9288..10031
                     /gene="zapD"
                     /locus_tag="QBF46_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDQ6673952.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRESQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            10041..10238
                     /gene="yacG"
                     /locus_tag="QBF46_001764"
     CDS             10041..10238
                     /gene="yacG"
                     /locus_tag="QBF46_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDQ6673953.1"
                     /translation="MSEIITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10454..10843)
                     /gene="mutT"
                     /locus_tag="QBF46_001765"
     CDS             complement(10454..10843)
                     /gene="mutT"
                     /locus_tag="QBF46_001765"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDQ6673954.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGKPWGKEGQPG
                     EWMALVDLNADDFPPANEPVIAKLKRL"
     gene            complement(10903..13608)
                     /gene="secA"
                     /locus_tag="QBF46_001766"
     CDS             complement(10903..13608)
                     /gene="secA"
                     /locus_tag="QBF46_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDQ6673955.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13670..14182)
                     /gene="secM"
                     /locus_tag="QBF46_001767"
     CDS             complement(13670..14182)
                     /gene="secM"
                     /locus_tag="QBF46_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDQ6673956.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14413..15330)
                     /gene="lpxC"
                     /locus_tag="QBF46_001768"
     CDS             complement(14413..15330)
                     /gene="lpxC"
                     /locus_tag="QBF46_001768"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDQ6673957.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15431..16582)
                     /gene="ftsZ"
                     /locus_tag="QBF46_001769"
     CDS             complement(15431..16582)
                     /gene="ftsZ"
                     /locus_tag="QBF46_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDQ6673958.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16643..17905)
                     /gene="ftsA"
                     /locus_tag="QBF46_001770"
     CDS             complement(16643..17905)
                     /gene="ftsA"
                     /locus_tag="QBF46_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDQ6673959.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17902..18732)
                     /gene="ftsQ"
                     /locus_tag="QBF46_001771"
     CDS             complement(17902..18732)
                     /gene="ftsQ"
                     /locus_tag="QBF46_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDQ6673960.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18734..19654)
                     /gene="ddlB"
                     /locus_tag="QBF46_001772"
     CDS             complement(18734..19654)
                     /gene="ddlB"
                     /locus_tag="QBF46_001772"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDQ6673961.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19647..21122)
                     /gene="murC"
                     /locus_tag="QBF46_001773"
     CDS             complement(19647..21122)
                     /gene="murC"
                     /locus_tag="QBF46_001773"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDQ6673962.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21176..22243)
                     /gene="murG"
                     /locus_tag="QBF46_001774"
     CDS             complement(21176..22243)
                     /gene="murG"
                     /locus_tag="QBF46_001774"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDQ6673963.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRVARA"
     gene            complement(22240..23484)
                     /gene="ftsW"
                     /locus_tag="QBF46_001775"
     CDS             complement(22240..23484)
                     /gene="ftsW"
                     /locus_tag="QBF46_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDQ6673964.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23484..24800)
                     /gene="murD"
                     /locus_tag="QBF46_001776"
     CDS             complement(23484..24800)
                     /gene="murD"
                     /locus_tag="QBF46_001776"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDQ6673965.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALHPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24803..25885)
                     /gene="mraY"
                     /locus_tag="QBF46_001777"
     CDS             complement(24803..25885)
                     /gene="mraY"
                     /locus_tag="QBF46_001777"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDQ6673966.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25879..27237)
                     /gene="murF"
                     /locus_tag="QBF46_001778"
     CDS             complement(25879..27237)
                     /gene="murF"
                     /locus_tag="QBF46_001778"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDQ6673967.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27234..28721)
                     /gene="murE"
                     /locus_tag="QBF46_001779"
     CDS             complement(27234..28721)
                     /gene="murE"
                     /locus_tag="QBF46_001779"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDQ6673968.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     NDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28708..30474)
                     /gene="ftsI"
                     /locus_tag="QBF46_001780"
     CDS             complement(28708..30474)
                     /gene="ftsI"
                     /locus_tag="QBF46_001780"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDQ6673969.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30490..30855)
                     /gene="ftsL"
                     /locus_tag="QBF46_001781"
     CDS             complement(30490..30855)
                     /gene="ftsL"
                     /locus_tag="QBF46_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDQ6673970.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30852..31793)
                     /gene="rsmH"
                     /locus_tag="QBF46_001782"
     CDS             complement(30852..31793)
                     /gene="rsmH"
                     /locus_tag="QBF46_001782"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDQ6673971.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31795..32253)
                     /gene="mraZ"
                     /locus_tag="QBF46_001783"
     CDS             complement(31795..32253)
                     /gene="mraZ"
                     /locus_tag="QBF46_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDQ6673972.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIYHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32855..33859)
                     /gene="cra"
                     /locus_tag="QBF46_001784"
     CDS             complement(32855..33859)
                     /gene="cra"
                     /locus_tag="QBF46_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDQ6673973.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33941..34027)
                     /locus_tag="QBF46_001785"
     CDS             complement(33941..34027)
                     /locus_tag="QBF46_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDQ6673974.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34039..34530)
                     /gene="ilvN"
                     /locus_tag="QBF46_001786"
     CDS             complement(34039..34530)
                     /gene="ilvN"
                     /locus_tag="QBF46_001786"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDQ6673975.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSGKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34533..36257)
                     /gene="ilvI"
                     /locus_tag="QBF46_001787"
     CDS             complement(34533..36257)
                     /gene="ilvI"
                     /locus_tag="QBF46_001787"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDQ6673976.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36376..36420
                     /locus_tag="QBF46_001788"
     CDS             36376..36420
                     /locus_tag="QBF46_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6673977.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(36575..37519)
                     /gene="leuO"
                     /locus_tag="QBF46_001789"
     CDS             complement(36575..37519)
                     /gene="leuO"
                     /locus_tag="QBF46_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDQ6673978.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9155 a        10494 c         9188 g         8932 t
ORIGIN      
        1 tttaacgatt attaaactat aaaaatccga attgaaccga ttcacttacc aattttgtga
       61 tttttaattc aataaaaacg aatttaaatt cattctatat attgagaggc gttgaggctg
      121 agctttacaa acggttcctt tttaagcaac tcatcttcaa ccatgcataa agcgggtgca
      181 ttcgctgccg cataccatta ttcttgatct gacggaagtc tttttgtaac aattcaaacg
      241 tctttgatgt aaacaaatta atacaacaaa cggaattgaa aacttacaca cgcatcactg
      301 cgtagatcaa aaaaacaatc accgcacgag gtttcatgat ggaaggtcaa cagcacggcg
      361 agcagctaaa gcgcggcctt aaaaaccgcc atattcagct tatcgcgctg ggtggcgcga
      421 tagggacagg gctattcctg ggtagcgcct ccgtaataca gtccgcaggg ccagggatta
      481 tcctgggtta cgccattgct ggttttatcg cctttctgat catgcgtcag ctgggtgaaa
      541 tggtggtcga agaacctgta gcaggctcct ttagccactt tgcttataaa tactggggca
      601 gttttgccgg tttcgcctct ggctggaact actgggtact gtacgtttta gttgccatgg
      661 cagagctgac tgccgtgggt aaatacattc agttctggta tccagaaatc ccaacctggg
      721 tttctgccgc cgtattcttt gtggtgatca acgccatcaa cctgaccaac gtaaaagtgt
      781 ttggcgagat ggagttctgg tttgccatta tcaaagttat tgcggtagta gcgatgatca
      841 tcttcggcgg ctggctgctg ttcagtggta acggcggtcc gcaggcaagc gttagcaacc
      901 tgtgggatca gggcggtttc ctgccgcacg gcttcaccgg gctggtgatg atgatggcga
      961 ttatcatgtt ctcgttcggt ggtctggaac tggtggggat caccgcagca gaagctgata
     1021 acccggagca aagtatcccg aaagcgacta accaggttat ctaccgcatc ctgattttct
     1081 atattggttc gttagccgtt ctgctctcac tgatgccgtg gacccgcgtt accgccgata
     1141 ccagtccgtt tgtgctgatc ttccacgagt taggcgatac ctttgtggcg aatgcgctga
     1201 acatcgtggt actgactgcg gcgctctccg tgtacaacag ctgcgtatat tgcaacagcc
     1261 gtatgctgtt tggtctggca caacagggta atgcgccaaa agcgctggcg tctgtcgata
     1321 aacgcggcgt accggttaac accattctgg tgtctgcgct ggttacagca ttgtgcgtat
     1381 tgattaacta tcttgctccg gaatcagcat ttggcctgtt aatggcactg gtggtatccg
     1441 cactggtgat caactgggcg atgatcagtc tggcgcatat gaagttccgt cgcgccaagc
     1501 aggaacaagg cgtggtaact cgcttccctg ctctgcttta tccgctgggt aactggatct
     1561 gcctgctgtt tatggcggcg gtactggtga ttatgctgat gaccccagga atggcgattt
     1621 cggtatacct gatcccggta tggctgatcg tgttaggtat cggctatctg tttaaagaga
     1681 aaaccgccaa agccgtaaaa gcgcattaat ctctctacgc cctcacccgt gcagggtgag
     1741 ggcaataatc tttacaccaa cgcgccgtaa atagtcagta gcgcaatcac taccacgacc
     1801 acgaacgagg ttttcttcgc cattgaaacc gctgccttcg gcgtctccac cttatcgaca
     1861 tgcggttcac gcgccagaga gaactgcgcc agacgcgtta acacctgata ctgcgaagta
     1921 tggaaatcac ccagcgaagc aaaccaggcc ggtaacgctt tctcaccatg accgatcaag
     1981 gcatatacca cacccgcaag acgaaccggc acccaatcca gtacatgaag cacggcatca
     2041 atgccggact gtaaacgatg atgcggcgtc tgatatcgcg ccagccagta ttgccatgca
     2101 cgcaaaaacg cataccccat cagcgtaacg ggtccccagg ttccccccac aatcagccag
     2161 aacagcggtg caagataaaa acgaaagtta atccacagca atgcattttg cagctcacgc
     2221 aaatactcac gttcgtcgca gcctgccggg acgccgtgaa tcatggtgag ttcgccagcc
     2281 atcgtggcac gggcatggct atcattacgt gaagcagctg tcagataagc atgataatga
     2341 agacgaactt tacctgcgcc aatacacagc aaaccaatca gcagccacac cagtagcgtg
     2401 ggaacgttga acaatactcc ctgcaatgcg cgtaacagta aaaacgtcac gcccatcgca
     2461 ataatggtca tgcctaacgt gcgcccgaga gaaaaatgtt tcacccgccg aaagaacgct
     2521 tcaagacgat gatcaagctg ccagtgctcg cccaacttaa acaggcgctc gaaaattaac
     2581 accagtaagg ttgtaaatag cgtcatgttg tctccttgct gaccagcgca cgaaaccgtg
     2641 cccaatcaaa tgcagggccg ggatcggttt tccgatccgg cgcaatatca caatggcccg
     2701 tcatgttatt agcgatatct ggatagcgat caatcagtgc ccgcgtaacc gccgcaagct
     2761 gttgatactg cgcatcggta tacgccagcg tatcggtgcc ttcaagctca atcccaatag
     2821 aaaaatcatt gcagcgttcg cgcccctgat actgagagac tcccgcatgc caggcacgtt
     2881 tatcgaaagg aacatactgg actatttcac catcacggcg aatcaaacag tgagcggaga
     2941 cgcgcaaatg ggcgatctca gcaaagaaag gatgtgcctg cggatcaata gttccagtga
     3001 ataatgcgtc gatccacgga ccgccaaact cgcctggcgg caggctaata ttgtgcacca
     3061 ccagcagggt gggtgtttcg tcatccgggc ggcaatcgta atgtggtgag ggaacgcggc
     3121 gcgcgccaac cagccacccc tgttctaaca acatgcagga tctccttata tgtggtgcta
     3181 atacccggtt cagagtagca tgtttctacc ttatgattcg ttagctatct ggagttttaa
     3241 catgccgcct cgccgctaca accctgacac ccgacgtgac gagctgctgg aacgcattaa
     3301 tctcgatatc cccggcgcgg tggcccaggc gctgcgggaa gatttaggcg gaacagtcga
     3361 tgccaacaat gatattacgg caaaactttt accggaaaat tctcgctctc atgccacggt
     3421 gatcacccgc gagaatggcg tcttttgcgg caaacgctgg gttgaagagg tgtttattca
     3481 actggcaggc gacgatgtca ccataatctg gcatgtggat gacggcgatg tcatcaatac
     3541 caatcaatcc ttgttcgaac ttgaaggccc atcccgcgtg ctgttaacgg gcgaacgcac
     3601 tgcgcttaat tttgtgcaaa ccctttcagg agttgccagt aaggtacgcc actatgtcga
     3661 attgctggaa ggcaccaaca cgcagttgtt ggatacgcgc aaaaccttac ccggcctgcg
     3721 ttcagctctg aaatacgcgg tactttgcgg cggcggagcg aatcaccgtc tggggctttc
     3781 tgatgccttc ctgatcaaag aaaatcatat tattgcctcc ggctcagtgc gccaggccgt
     3841 cgaaaaagcg tcctggctgc acccggatgc gccagtagaa gtcgaagtag agaatctgga
     3901 agaacttgat gaagccctga aagcaggagc cgatatcatc atgctggata acttcgaaac
     3961 agaacagatg cgcgaagccg tcaaacgcac caacggcaag gcgctactgg aagtgtctgg
     4021 caacgtcact gacaaaacac tgcgcgaatt tgccgaaacg ggcgtggact ttatctccgt
     4081 cggtgcgcta actaaacacg tacaagcact cgacctttca atgcgttttc gctaattgct
     4141 gacagattta tccgctccat cgcgggcgga taatctcccc ttccccaact ttcttcgtaa
     4201 cgcctcgcaa atttcttgtc tttcgctgca acgaaataaa aaacctcgga agctgctttc
     4261 ccgtttcgtt ttttgcccct cgcttgtgct gatggcgttt gccaaagtag cgccaaccaa
     4321 atcaaggagc gaaacagatg gacaagcaac gcggttttac acttatcgaa ctgatggtgg
     4381 ttattggcat cattgccatt ttaagcgcca ttggtattcc cgcttatcaa aactacctgc
     4441 gcaaagctgc actcaccgac atgctacaaa cctttgtgcc ttaccgtacc gccgtagagt
     4501 tgtgcgcgct ggaacatggt ggattagata cctgcgacgg tggcagcaat ggcattccct
     4561 cgcctaccac cacccgctat gtttcagcca tgagtgtggc aaaaggcgtg gtgtcgctga
     4621 ccgggcaaga aagtctcaat gggctaagcg tcgtcatgac gccgggttgg gataacgcaa
     4681 acggcgtcac cggctggacg cgcaactgca atattcaaaa tgacagcgca ttgcagcaag
     4741 cctgtgaaga tgtcttccgc tttgatgacg ccaactaagg agcggcaatg aatattccac
     4801 agctcacggc cctgtgcctg cgttatcagg gagtcttgct ggatgccagc gaagaagtgg
     4861 ttcatgttgc ggtggtcgat gccccctcac atgagttgct ggacgcattg catttcgcta
     4921 ccaccaaacg tattgagatc acctgctgga cgcgccaaca aatggaaggt cacgccagtc
     4981 gcacacaaca gacattgccc gtagctgttc aggagaagca tcagcccaaa gcagagttgc
     5041 tgactcgaac gttacaatct gcgctggaac aacgcgcgtc tgatattcat atcgaaccag
     5101 cggacaatgc ctaccgcatc cgcttgcgta tcgacggcgt attgcatcct ttaccggatg
     5161 tttcaccgga tgccggagtc gcattaaccg ccagattaaa agtgctggga aacctggata
     5221 ttgcggaaca tcgcctgccg caggacgggc aattcactgt cgaactggca ggaaacgccg
     5281 tctcatttcg tattgcgacc ttaccatgtc ggggtggtga aaaggtggta ttaaggttgt
     5341 tacagcaggt gagccaggca ctggatgtta acacgctggg aatgcagccg ttacaactgg
     5401 cggactttgc tcatgccttg caacaaccac agggactggt gctggtaact ggccctaccg
     5461 gcagcggcaa aacggtcacg ctttatagtg ccctgcaaaa gctgaatacc gctgacatta
     5521 atatttgtag cgtcgaagat ccggttgaga tccccatagc cggactaaac cagacgcaaa
     5581 tccatccgcg tgccgggctc acctttcagg gcgttttgcg tgcgttattg cgccaggatc
     5641 ctgacgtcat catgatcgga gagatccgcg atggcgaaac ggcagaaatt gccattaaag
     5701 ccgcgcaaac cggtcacctg gtgttgtcta ccctacacac taattccacc tgcgaaacgc
     5761 tggtacgttt acagcaaatg ggagtcgccc gctggatgct ctcatcagcg cttacgctgg
     5821 taatagccca gcgtctggta cgtaaacttt gcccacattg tcgccagcag caaggggagc
     5881 ccatccatat tccagacaat gtatggccgt cgccgctgcc ccattggcag gcacccggtt
     5941 gtgtacattg ctaccacggt ttttatggtc gcactgcctt atttgaagtt ctgcccataa
     6001 caccggtcat acgtcagctt atttccgcta ataccgacgt tgaatcgctg gaaacgcacg
     6061 cccgacatgc gggtatgcga acgctttttg aaaacggctg cctggccgtg gagcaaggct
     6121 taaccacctt tgaagagtta atccgcgtac tggggatgcc gcatggcgag taagcaactc
     6181 tggcgctggc atggcataac cggcgacggc aatgcgcaag atgggatgcg atgggcagag
     6241 agccgtgctt tgctgctcat ggcactacag caacagatgg ttaccccact aagcctgaag
     6301 cgaattgcta tcaattctgc gcagtggcga ggagataaaa gcgcggaagt cattcatcaa
     6361 ctggcgacgc tacttaaagc cgggttaacg ctttctgaag ggctggcact gctggcggaa
     6421 cagcatccca gtaagcaatg gcaagcgttg ctgcaatctc tggcacacga tctcgaacag
     6481 ggcattgctt tttccaatgc cttattaccc tggtcagagg tatttccgcc actctatcgg
     6541 gcgatgatcc gcacgggtga actgaccggt aagctggatg aatgctgctt tgaactggcg
     6601 cgtcagcaaa aagcccagcg tcagttgacc gacaaagtga aatcagcgtt acgttatccc
     6661 atcatcattt tagcgatggc aatcatggtg gttgtagcaa tgctgcattt tgttctgccg
     6721 gagtttgccg ctatctataa gaccttcaac accccactac cggcactaac gcaggggatc
     6781 atgacgctgg cagactttag tggcgaatgg agctggctgc tggtgttgtt cggctttctg
     6841 ctggcgatag ccaataagtt gctgatgcgc cgaccgacct ggcttatagt gcggcagaaa
     6901 ttgctgttac gcatcccgat tatgggttca ctgatgcggg gacaaaaact cacgcagatc
     6961 tttacgattc tggcgctgac acaaagtgca ggcattactt ttttacaggg cgtagagagc
     7021 gtcagagaaa caatgcgctg cccgtactgg gtgcaacttc tgacacaaat ccagcacgat
     7081 atcagtaacg gtcaacccat ctggctggcg ctaaaaaata ccggtgagtt tagcccgctc
     7141 tgtttgcaat tagtgagaac aggagaggca tccggctctc tggatctcat gttagacaac
     7201 ctcgcccatc atcatcggga aaacacaatg gcgctggcgg ataacctcgc agccttactg
     7261 gaaccggcgt tgctgatcat aacgggagga attattggta cgctggtggt ggcaatgtat
     7321 ctgccaattt tccatttagg cgatgcgatg agtgggatgg gataacgctg gcgtgttgct
     7381 ccacgccagc gttgggagat tacaggttgt tgaagatgcg gttttcttgt tcctgcacac
     7441 gaataaacgt ggtgcgcttg gtcagctctt tcaggcgtga agccccaacg tatgtacaag
     7501 ctgaacgcag gccgcccaaa atatctcgcg cggtattttc aaccgggcct cgcagcggca
     7561 gcttaacggt tttaccttct gctgcgcgat attccgcaac gccgccaacg tgacgtttca
     7621 tcgcagactc ggagctcatg ccgtagaaca gcataaattt ctcgccgttc tcctcaacga
     7681 tgcgaccgcc gctctcttcg tggcccgcca gcatgccgcc aagcatgacg aaatcggcac
     7741 cgccgccaaa ggctttcgcc acatcgcccg gcgtggtgca gccaccatcg ctgacgatca
     7801 ttccgcccag accgtgcgca gcatcggcac attcgattac cgcagaaagt tgcggataac
     7861 cgacgcctgt tttgacgcga gttgtacaaa cagaacctgg gccaatgcca actttaacga
     7921 tatcggcacc tgagaggata agctcctcac acatttcacc agtcactacg ttaccagcac
     7981 aaatggtttt ggtcggccac gcttcacgcg ctttcgcaac gaactgcacg aagtgttcgg
     8041 aataaccatt cgccacgtca atacaaacga agtttaatgc cgggttcagg tcgagaatct
     8101 gtttagtttt ttcgaaatcc gcatcagacg taccggtaga aaccatcaca tgtttcagca
     8161 catcagcgga agaattgttg ataaacgctt gccactcttc gacagaatag tgtttatgca
     8221 cagcagtcaa aatatcaaaa gaagccagcg cagaggccat agaaaatgtg cctacggtgt
     8281 ccatatttgc ggcgataatc ggcacgccgg accagctctg acctgaatgt ttgaaggtga
     8341 attgacgttc cagttcaaca tcggaacggc ttttaagagt ggagcgttta gggcggatga
     8401 gaacgtcttt aaaacctaac ttcagatctt cttcaatacg catgtgcgga ttcctggggt
     8461 taatggctat aagggtaaga gcgcaactcc agtgacgcta tcatacgcac taatcaatgc
     8521 ggcgcaagac tgcgaaattg tcttttttta cgctacaatc ccataaattt acctggtaaa
     8581 tagtgagcga acgtttcatc aatttcgctt tcgtgcactc attattaacg ttttgataat
     8641 catgatgaat tcccgggaat ataaattatg aggtatatag ttgccttaac gggaggcatt
     8701 ggcagtggca agagtaccgt tgccaatgcg tttgctgatc tcggaattaa cgtcattgat
     8761 gccgatatta ttgcgcgtca ggtggttgaa ccaggtgcac ctgcgctaca tgccattgct
     8821 gatcactttg gcgctaacat gattgctgct gatggaacat tgcagcgccg ggccttgcgc
     8881 gagcggatct tcgccaaccc ggaagagaaa aactggctta acgccctgct gcatccgctg
     8941 attcagcaag agacgcaaca ccagatccag caagctactt ccccctatgt actgtgggtt
     9001 gtgccattgc tggtagaaaa ctcactgtat aaaaaagcga atcgagtgct ggtggtggat
     9061 gtcagcccag aaacgcaact taagcgcacc atgcagcgcg atgatgtaac tcgcgagcat
     9121 gtcgaacaaa tccttgctgc tcaggcaacg cgcgaagccc gccttgccgt ggcagatgac
     9181 gtcattgata ataacggcgc accggatgct atcgcatcgg atgttgcccg cctgcacgca
     9241 cactatttgc agcttgcgtc gcagtttgtc tcacaggaaa aaccgtaatg cagacccagg
     9301 tcctttttga acatccacta aatgaaaaaa tgcgtacatg gctgcgcatt gagtttttga
     9361 ttcagcaact caccgttaat ttacccatcg ttgaccacgc tggcgcgctg catttcttcc
     9421 gtaatgtcag tgaattactg gatgttttcg agcgcggcga agtccgcact gagctgttga
     9481 aagaacttga ccggcagcaa cgtaaactcc agacctggat tggcgtgcct ggcgtggacc
     9541 agagccgtat tgaagcatta attcagcagt taaaagcggc ggggagcgta ttaatttccg
     9601 cgccgcgtat cgggcaattt ctgcgtgaag atcgtttgat tgctctggtg cgtcagcgac
     9661 tgagcatccc aggcggctgt tgcagctttg atttacctac attgcacatt tggctgcatc
     9721 taccccaggc gcagcgcgaa agccaggtag aaacctggat tgccagcctg aacccgctca
     9781 cccaggcact taccatggtg ctggatttaa ttcgccagtc ggcccccttc cgtaaacaaa
     9841 ccagcctgaa tggtttttat caggataacg gtggcgatgc cgacttgctg cgcctgaatc
     9901 tgtcgctcga ttcacagctt tatccgcaaa tttccggaca taagagccgt tttgccattc
     9961 gttttatgcc gctggacact gaaaacggac aggtaccgga acgtctggat ttcgaactgg
    10021 cctgttgcta aggagtaaaa atgtcagaaa ttattacggt gaattgccca acctgcggga
    10081 aaacggtggt gtggggtgaa atcagcccgt ttcggccatt ttgctccaaa cgttgtcagc
    10141 tgatcgacct cggagaatgg gctgctgaag aaaaacgaat ccccagcagt ggcgatcttt
    10201 ccgaaagcga tgactggagc gaagagccaa agcagtgaca tttgtctgat gccgcacgta
    10261 ggcctgataa gacgcggaca gcctcgcatc aggcatcttg tgcaaatgtc ggatgcggcg
    10321 tgaacgcctt atccgaccta caaaaacaaa tttatcaatg agttagaaat ccctgtaggc
    10381 ctgataagac gcgccagcgt cgcatcaggc atcttgtgcg aatgtcggat gcggcgaaaa
    10441 cgccttatct gacctacaga cgtttaagct tcgcaattac cggttcattg gctggcggaa
    10501 aatcatcggc attaagatcg accagcgcca tccactcacc gggttgccct tctttacccc
    10561 acggcttccc ttcccagcgt tcgaccagcc agaaccacag tgttatatgc ctgtccggga
    10621 attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct
    10681 gaagttcacg caccaccgcc tgttccggcg tttcacccat ttcaatttta ccgccgggaa
    10741 actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt
    10801 tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa
    10861 aaaggcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg
    10921 gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg
    10981 ttcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag
    11041 ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac
    11101 ttcttcaggc atacgtacct gaactttgct cagcgtactg ataacttcat atttcaacga
    11161 ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt
    11221 ctgtgcatag cctcgcaggt ggataccctg acgcagatag tccatcgctg ccaggtgctc
    11281 tttccacagg gagtcaagcg tttgcagcat gacgcctttc tcgaagtgac gcatcatctc
    11341 tgcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca gaatgcgctc
    11401 acgcagcgtc tcttcatgca gttcgggttc tttatccagc cactcagcaa ttggtaaatc
    11461 gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag
    11521 cgactgcggc ggaatgtagg catcaatggt ggctttgaac acatcttcac gaatgctgtt
    11581 gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg
    11641 acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg
    11701 actttcaact ttacgctggg cgttggcaat cgctttggtc acccacgggt gttcaatggc
    11761 ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat
    11821 acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg
    11881 accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat
    11941 gatatgcagg ccacctgctg ccagtaccgc atcgtgacgt acctgccagt cggctttaat
    12001 tttttcaatt tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc
    12061 accgagcaca atatctgtac cacgacccgc catgttggtc gcgatagtca ccgcagccgg
    12121 ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacatt
    12181 gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat
    12241 agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc
    12301 ctgaattttt tccgcttcag tcatgtagac caggtccggc aaatctttac gaatcattgg
    12361 acggttggtc ggaacaacga cggtatccag tttgtagatg gagctaaatt cgaaagcttc
    12421 ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa
    12481 ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac
    12541 agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc
    12601 aacgatgata acttcaccat ctttaacgat gtagtcgacg tcacgggtaa acagcgcatg
    12661 agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga
    12721 ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg
    12781 ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa
    12841 ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt
    12901 atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc
    12961 gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc
    13021 ttcagggctg aacgccatgt tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc
    13081 gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt
    13141 gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg
    13201 cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta
    13261 agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg
    13321 ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg
    13381 cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc
    13441 tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt
    13501 ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt
    13561 gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa taaaatctca
    13621 aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc
    13681 gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg
    13741 cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct
    13801 gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag
    13861 attcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg
    13921 tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt
    13981 tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg
    14041 ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac
    14101 tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc
    14161 agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg
    14221 cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag ttgtgcggaa
    14281 aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa
    14341 aacgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca
    14401 gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc
    14461 gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag
    14521 tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc
    14581 acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa
    14641 acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct
    14701 gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt
    14761 acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg
    14821 gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt
    14881 gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg
    14941 aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa
    15001 cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt
    15061 atcgatgccc aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc
    15121 atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg
    15181 gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc
    15241 agggcgtaac gtcagggtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac
    15301 gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac
    15361 cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc
    15421 cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct
    15481 ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg
    15541 tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat
    15601 tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta
    15661 cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt
    15721 cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga
    15781 agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca
    15841 gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc
    15901 cagaacccat cattgcgtag cccatttcag acattacggt gcgtacgtct gcaaagtcca
    15961 cgttcatcaa acccggacga gtaatcagtt cggcgatacc ttgcacagcg cctttcagta
    16021 catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca
    16081 gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct
    16141 gctcggcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa
    16201 cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg
    16261 taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca
    16321 atgcatcgcg atcctcatca gccgcattgc ggccaacttc tggattagcg ccagcgccca
    16381 gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg
    16441 cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt
    16501 gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg
    16561 taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta
    16621 atcatcgtcg gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga
    16681 tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg
    16741 actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat
    16801 ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct
    16861 gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg
    16921 ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt
    16981 tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt
    17041 gcagactccg cggcggacga ccacctacgc tcggcacttc cacgctctca tcttttccaa
    17101 cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg
    17161 tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag
    17221 tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga
    17281 cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc
    17341 cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt
    17401 tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa
    17461 gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat
    17521 gcagcacacg atgctcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca
    17581 catcttcttg cgttacttct tcttcagaaa taggcaccat accaatttca ttctggcagc
    17641 tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt
    17701 ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc
    17761 ctttatccat accacgcgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg
    17821 gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca
    17881 gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt
    17941 agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc
    18001 aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc
    18061 tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt
    18121 attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct
    18181 gtcctttgcc agcatctgcc ccatttcgcg atacccctgc aacacttcat tagcgctgcc
    18241 ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa
    18301 ggtatttcct tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc
    18361 aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca
    18421 cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc
    18481 tggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc
    18541 accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac
    18601 gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag
    18661 acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc
    18721 agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca
    18781 tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat
    18841 tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc
    18901 ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg
    18961 acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat
    19021 aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta
    19081 tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct
    19141 gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca
    19201 cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg
    19261 ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg
    19321 cgaccggtaa accggcacct tgccatagaa gtttgctgcg tagtttatcc attgaaagcg
    19381 cagatgccat cactccgctt ccggtataag gcaagcccat cagctcgagc atcccctgca
    19441 gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aagcccatcg
    19501 acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcgtca ataccgcctt
    19561 cacgcagtcc ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggagg
    19621 tcccacccaa caggaccgcg attttatcag tcatgttgtt cttcctccgg agtttgcggc
    19681 ttcagtttga tttcagctaa agaacgagca atttttccaa tattaccagc cccctgaacg
    19741 agaatcaggt cgttaccggt taataccggt gccagcatct cggctacccg cgccggatcc
    19801 ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg
    19861 tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca
    19921 acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc
    19981 ggctgaaaca gcattaccag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg
    20041 gcgtccactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta
    20101 ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg
    20161 aagctttcaa gcgcccgcaa aatagcctcg tcgtcaatgc cctcttccgt agcaaccgca
    20221 accgcagctg cggcgttcag cgcgttatga cgacctggcg cattcagggt gacgcgcatc
    20281 ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct
    20341 tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg ccccactcgc
    20401 ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc
    20461 aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta
    20521 tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac
    20581 gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga
    20641 acccccgccg cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg
    20701 tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttccggc aatggcgatg
    20761 ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc
    20821 gcttcatgag cggcgacaat ttccgggtta tcggcagaaa tcgcgctgga aacaacgacc
    20881 acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc acccagattc
    20941 attaactgct gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca
    21001 ttggccagaa cttcggcaat accgcccata ccggcaccac caatgccgac aaaatgtatg
    21061 tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc
    21121 attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg
    21181 cccgggcaac ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag
    21241 cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg
    21301 cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca
    21361 gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg
    21421 gtagtcctgc cgcggcgatt tcactcaccg ttaacgcacc ggagcggcaa acgacgacat
    21481 ccgcccacgc atacgccgcc gccatatcat caataaattc agtcacttta tgctgcggtt
    21541 gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttgccg ctctgatgcc
    21601 agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc
    21661 gtgcgccctg ggaaccaccc actaccaaca cacgaaccgg accttcacgt ccagccaaac
    21721 gttgctgcgg caacggcagc gccagcacat cagtacgcac cgggttaccc actacttccg
    21781 cattagggaa agcacctgga aacgcctgca tcactttggt ggcaatcttt gccagccatt
    21841 tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc
    21901 acgcggccag accaccagga cctgatacgt agcctcccat accgagcacc acgtcaggtt
    21961 tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggggcag
    22021 ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga
    22081 tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt
    22141 gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg
    22201 ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa
    22261 cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt
    22321 cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt
    22381 cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc
    22441 aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaatgctt tacggccaat
    22501 cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata
    22561 ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc
    22621 cagtttttgt accgagttac ctaaaccttg cccccaaagt tcgccgcgac caaacgccat
    22681 cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc
    22741 ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc
    22801 cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag
    22861 cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc
    22921 caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc
    22981 gcctttacgc accagatagt tggcgatata gcaaaacagc gacagttttg tcagctccgc
    23041 aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc
    23101 cactaccaga acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg
    23161 ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaataaacg ccaaaatcag
    23221 atagacacca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg
    23281 cattgacgcc gaggtcacca tgataaagcc aatcgccgcg aggccgaagg tcagccacag
    23341 taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca
    23401 gccctttagc gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt
    23461 caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg
    23521 cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga gagtagaacc
    23581 atatcgcccg gctgaacacg cggagccagc aagcgcatcg cctgttccat agtttcggtt
    23641 tgttctgcca cttccgggtg tagcgccgcc agctgcgcgc cgtcacgacc gaaacaatac
    23701 agacgtacgt tatcgccatt caggtaacgc gccagtgggc taaagtccgc cgatttacca
    23761 tcgccgccca gcaacaaatg cagcgtgccg tctacgtgca ggccattcag cgccgcttcc
    23821 gtactgccga cgttggtcgc ttttgaatcg ttaatccaac gtacgccgtt atgctccagc
    23881 acaacttcaa agcgatgcgg cagaccagtg aatgtggtta acgctttcag gctgctggca
    23941 cgcggtaacc ctgcggcatc tgccagtgcc agcgccgcca gcgcattggt gtagttatgc
    24001 tgcccggaaa gtttcatctc tttcacgttc agcactttct cgcctttaac ccgcagccag
    24061 gtttcgccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag
    24121 cgttcatccg caccgcgaat cggcattgtt aaggcatcat cggcattaac cacgcaaact
    24181 tttgcgtttt cgtaaatgcg cagttttgct gcacgatact gttgtaaacc aaacggatag
    24241 cgatccatat gatcttcagt cacgttcaga atggtcgctg ctaccgcctg taagctagag
    24301 gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc atcatccagc
    24361 agcatcaacg caggcaggcc aatattgcca cccactccaa cgttaacccc cgccgctttc
    24421 gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca
    24481 atcggtgctt gtgcttcgcg acaaaacagc tcgatatcgc caacgatttc gattccggca
    24541 tcagcggcag cgcttaagga tggatgcgcc agtgcaatac cgggactggc gacaatcaga
    24601 tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt
    24661 aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga
    24721 gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca ggccgataat gacaacattt
    24781 ttaccctggt aatcagccat gattaacgta ccttcagcgt tgccagacca atcaggacca
    24841 gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt
    24901 catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc
    24961 cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca
    25021 ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgacc
    25081 cgacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg
    25141 ccccgactat cgcggtacag acaataacca gttccccggc gtgtcgcaga tacggtatgt
    25201 gtaaatagct ggcaaagttc atgttgccgg tcgcccacgc caccagcgca aaaccaccgg
    25261 cgacaaatac ggtcggcata attgccagac cgtcaagacc atcggtcagg tttaccgcgt
    25321 tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc agttgcggca
    25381 tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt
    25441 acaaagcgaa agcaacaccc agcgcaatga ccgacatcca gaaatatttc caacgagcga
    25501 tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac
    25561 cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca
    25621 gcagcacgga gatcacaatc gctgtcagga tcataatccc gcccatggtc ggcgtaccgc
    25681 gcttgctgaa gtgtgattca ggaccgtcgt tacgcactac ctgaccaaag gaaagttttt
    25741 gcaaatgagc aatcatgcgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc
    25801 tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca
    25861 aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc
    25921 catggcagca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag
    25981 tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc
    26041 ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt
    26101 tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc
    26161 gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat
    26221 tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat
    26281 ggggaacaga cggccaggaa cagctttcag atttgccagc cccgctttga tagcatcaag
    26341 cgttgcgccc acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg
    26401 ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt
    26461 accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga
    26521 gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc
    26581 ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt
    26641 cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag
    26701 cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc
    26761 aagttcaata actgcgtaat cgtattccgg cgttaagcgc aacagcgtca tcggtacacc
    26821 gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatagc
    26881 cgccgtcatc tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc
    26941 cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac
    27001 gattaactgc ggcaggtcga tgtccagcgg acggctaacc agtagtgcgc ctgcgccgcc
    27061 agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa
    27121 caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc
    27181 accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc taatcatgca
    27241 atcaccccca gcaaacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca
    27301 acaatctggt aatcctcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc
    27361 tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca
    27421 tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta
    27481 tcgtccgtca ccaccgccac gtcagcaaat tcttcggcaa ttgcgcccat cagtggacgc
    27541 ttacctttat cgcgatcgcc accacagcca aagacacacc acagcttgcc cgcacagtgc
    27601 agacgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc
    27661 accgtcggtt tgccaggtgc agtaaacact tccatacgtc cgcaaaccgg ttgcagacgc
    27721 gcggcggttt tcagcagatc agccaacgga tagcccagtg ccaacagcgt cgccagcgcg
    27781 agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tctcaatttc gccatcgccc
    27841 caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc gttcgctttt
    27901 aaccagcgtc cgtgacagtt cggattgata tgatcttcca ttgataccgc aaccgcgtcc
    27961 ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg
    28021 caatgatgct cagaataaag cagccatttc gcggcttcgt agtgttccat atcaccatga
    28081 taatcaaggt gatcgcggct taagttcgta aagaccgacg ccgcaaattt caatgccgcc
    28141 acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctga
    28201 tccaccagcc ccgccagctc atgctgaaca tcgactgccg aaccggttgt attttctgtc
    28261 gggatcactt tccccagcag gccgttacca acggtgccca ttaccgcgct ggtttcgcca
    28321 agcagttggc tccactgcgc caacagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt
    28381 acgcccacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat
    28441 aaacgctcgt tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca
    28501 ccatcggtcg cctcatcttt cgcctctgca ataatggcag ccacaccttg cgctatcgcc
    28561 tgcgggatat atcgacgccc gtccgcctga tgacctacta cagctacaaa gagatcgccc
    28621 gccgcagcca cacggctgtc gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc
    28681 acccacggag caagaaggtc gcgcaaatta cgatctgcca cctgtcccct cgccttgatt
    28741 aatcacaaat tcatttttat cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg
    28801 caatacgccg cccatgatgg caccaaagac cggcgcggaa acggcgccgc cgtagtattt
    28861 acccgcctgc ggatcgttga taacaacaac cagcgcgaag cgcggctgac tcgcaggtgc
    28921 aacgcctgcg gtataagcaa tatatttatt gatgtagcga ccgtccggcc cgaccttttt
    28981 cgcggtaccg gttttaatgg cgatacgata gcctttaatc gccgccttca cgccgccgcc
    29041 gcctggtagc gccacgcttt ccatcatatg caccacagtg cggacaatgg attccgggaa
    29101 gacacgttca ccgggaaccg gggggtcaac tttggtaatc gacagtgggc gataaatgcc
    29161 gtagctgccg atagttgcgt agactcgcgc taactgtaac ggtgttacca ttagcccgta
    29221 gccgaaagag aaggtggccc tctctatgtc agaccaccgt tgtttttgag gatataagcc
    29281 actgcgttct ccgaccaacc ccaaattggt cgcttttccc agtccaaaac gtgagtaagt
    29341 atctactaac gctgaggacg gcatcgctaa cgccagcttg gaaacaccga cgttactcga
    29401 cttctgtaat accccggtca gggttaattc gctgtagcgt gccacgtctt tgatttcgtg
    29461 gccgttaatt cgataaggaa tggtattgag taccgagttt tcccgcacca cgccacgttg
    29521 caacgcggtc attaccacca tcggtttaac cgttgagccc ggttcaaaca cgtcggtgat
    29581 ggtacggtta cgcatcgcct ctttcggcgt gccgctcaga ttgttagggt tgtatgacgg
    29641 gctgttagcc atcgccagca cttcaccggt gttgacatcc accagcacgg cgctaccaga
    29701 ttcagccttg ttaaaggcca ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg
    29761 ttcatcaata ctcagcgcca ggttgtgcgc tgcctggctg tcagtagaag aaatatcttc
    29821 aattacgcga ccatagcggt ctttacgcac aatgcgctca cccggctgcc cggtaagcca
    29881 tttatcgaaa ctcttctcaa cgccctcaat cccttgacta tcgacgttag taaagccaat
    29941 gaggtgagca gtcacttcgc cggatggata ataacggcga gattcttcac gcagatgaat
    30001 ccccggcagt ttcagttttt tgatgtagtc cgccatgtca gggtttacct gacgcgccag
    30061 ataaataaag cgccctttcg ggttggcgtt aatgcgggca gaaagctgat ccagcggaat
    30121 attgagcgcg ttagccagcg ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg
    30181 cacttctttc gggtcagccc aaatcgcttt taccggcacg ctcaccgcta acgggcgacc
    30241 agaacggtca gtaatcatgc cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat
    30301 gtcgccctct ttcaccagca tatccgggga gataacttgt aaccacgcta cgcgtccgag
    30361 cagaaaagcc agcgccagga gaatacagcc gcataacaac gcaaaacgcc aactgataaa
    30421 gttggcatgt tcttcctgac gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg
    30481 cgtttatcct tatttttgca ctacgatatt ttcttgtgac ggatcaacat gctgcatttg
    30541 cagcttttcc gtggcgatcc tttccacccg gctatggtcg ccgagcgcat tctcttcaag
    30601 gatcaggttg cgccattcaa tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg
    30661 agcggtcagt aaacgggtat ggtgcgccgt ggttaccaca gtcaccgccg tcaaaataat
    30721 gcaaatgaac aggcagagtg gcagcttccc aaatcgcaaa agatcgtcac cgataacacc
    30781 aggcaatgca tggcgctcgt ggcttcccat cgatccttta actttgctta gagcttctgt
    30841 cactctgctg atcatgcatt cgtcctctct gcaatacgca gaactgaact acgggcacga
    30901 gggttctcag ccacctcttc ttcgcccggc attaacttgc ctagtgctcg cagctgacgg
    30961 ccacccagtt ttttgagctg ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg
    31021 cggctgtttt cacgcataaa acgtttcaca atacggtctt ccagcgagtg gaagctgatg
    31081 atcgaaagcc gcccacccgg ggccagcacg ttgagcgagc tttttagcgc ctgctctatc
    31141 tcctccagtt cactgtttac ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt
    31201 ttaaacttat ctttcaccgg cgttgcagca gccacgactt ccgccagttc tttggtgcgg
    31261 gtcatcggct gttcgcggtt acgctcgaca atggcgcggg caatgcgttt ggcaaaacgc
    31321 tcttcaccat aggttttcaa tacccaggcg atatcggctt cttctgcggt ttgtagccat
    31381 tcagcggctg actgcccacg ggttgggtcc atacgcatgt ccagcggacc atcgcgcata
    31441 aaggaaaagc cgcgctcagc atcatcaagt tgcggtgaag agacgccaag atcgaggaga
    31501 atgccgtcga tcttgccgat aagatcgcgc tcggcaacgt attcgcccag cgcggaaaaa
    31561 ggtccgtgga tgatggagaa gcgcggatca tcaatagtct tcgcaacggc gatagcctgc
    31621 gggtcgcgat cgatcgccag caaacgcccc tcttcgccaa gctgcgagag gatcagacgt
    31681 gagtgaccac cgcgaccaaa agtcccatca atgtagatgc catcaggacg gatattgagg
    31741 ccattaacgg cttcatccag cagcaccgta gtatgtttat agttttccat cattttatag
    31801 agacaagtcc tgcagtcgct ccgataagtc tccggtagcc aactgctctg cgtcgatatc
    31861 ttccttgacc tgttgatgcc aggttgtttc atcccacagc tcaaacttgt tgaactgtcc
    31921 aaccagcatc acttcttttg tcagcccggc atgttgccgc agtactggcg cgattaacaa
    31981 tcgacctgcg ccatccatct gacattcgct ggcatgacct aacagtaggc gctgcacacg
    32041 gcgctcaacc gggttcatgc tcgacagacg cgataatttt tgctcgataa tttcccattc
    32101 aggcaggggg taaagcagca ggcacgggtg ataaatgtca atggtgcaaa ccatttgacc
    32161 ggcagcgttc tcaagcagct gttcccgata acgggtaggc actgataagc gccctttgct
    32221 gtcgagattg actaacgttg ctccccggaa catgccagcc tcacccctta ttcccacttt
    32281 gccccacaat ttcccactga aaggagttta cggagctgag gaaaagcttg tcaagccagt
    32341 cacaaggcat accgaggtaa aaaacccgat gtttacggct aatatcagcg ttgtttattt
    32401 tacgtccaac gtgcgaagca aattaacgtt atgaatagct gtaagaaaaa atccctcatc
    32461 gtcatcactg ttttaaaatg actcactatt ttccacagca aatataaagt gtcagtttgc
    32521 gacgcgagcg gcattttagg acatatcttc cccggttaac agtccttgtt acgtctgtgt
    32581 ggcgcttgat gccagcgggt ttgcgcaaaa gcaaagaaaa gtgtttgtta aatcggcaat
    32641 tcatcgccgc atgtaacaaa ataatgcaaa aacgcgctgt cgatatcact taattattgt
    32701 gaatttcagc tggtgagtta attaacgggc gggtttaaaa gcatttatca ataatagtca
    32761 ggaatagtct tatttacttt aagcatattg atgtccagtc ccgtactcta cgcgccagag
    32821 ggaaattcac ctggcgcgta tttttgttcg cggcttagct acggctgagc acgccgcggc
    32881 gatagagatt acgtttaatg cgcgttaaac caggttttgg cttacgcggt tcgtccaggc
    32941 ttgccaggac aatctccagc acacgctctg cgacatcgcg gtgacgttga gccactgcca
    33001 gcaccggaca ctgtaagaag tcgagcagtt cgttatcgcc aaaggtggca attgccaggt
    33061 cagaaggcag tttgccgtcg cgacgcagcg tgacatccat cactccttgc aacaacgcaa
    33121 acgacgttgt gaacagcgcc tgcggcatcg gatgcgtttc cagccatttt tcgaataact
    33181 gggcagccgc ctcccgctca tagctgttgg catacaggaa atgcacttcg cgcggatcat
    33241 ctttccaggc agtacggaaa ccttgttcac gcaggaagct gacagaaagc tccggtagcg
    33301 caccaagata aagcaccgtc tcggcgggaa acttacgtaa ctcttccgcc agcatttcgg
    33361 catcatcctg atcggcacca accacgctgg tgaagtgttc acgatcgagg gcgcggtcca
    33421 gcgcgacaat cgggaacggg tcgttagccc agcgttgata aaaaggatgc tcaggaggca
    33481 acgacgtcga aacaataatg gcatcaacct gacgctgtaa aaggtgctca atgcaccgca
    33541 tttcgttgtc tggctgatct tctgagcagg caatcagcag ttgataaccc cgttgccgcg
    33601 cctggcgttc aagatagtta gcgatgcggg tatagctggt gttctccaga tcggggatca
    33661 caagaccaat agaacgtgtg cgtccagcac gaagcccagc tgccacggcg ttcgggtggt
    33721 aattgtgctc acgcaccaca gccatgactt tttcaacggt tttgtcgctc acacggtatt
    33781 gcttcgcttt gccgttaata acatagcttg cagtggtccg cgacactccc gccagccgag
    33841 cgatttcatc cagtttcaca attgcccctt gcgtaaaatg taaaaaccat agccattgta
    33901 ctggtatggg ttaaattcat taacatctaa gcgcagaaaa ttaactgcgg caactgcttt
    33961 tattccaggt tcccactgat ttcgcaaaaa aagcccaacg tgacatgttg ggctgtaaat
    34021 tgcgcattga gatcattctc aacgcattat tttatcgccg cgcgaaagtc cgaccacacc
    34081 agagcgagca acctccacaa ttttcgccac atcgcgaatc gatgctaaaa atgcatcaag
    34141 cttaccgctg gtgcctgcta attgaacggt ataaagcgag ggtgtgacat cgataatttg
    34201 cccacggaat atttccgtat tacgtttcac ttcgtcacgc ccgtaaccgc tggcctgaat
    34261 tttcaccagc atgatttccc gctcaacatg cgcgccctgc cccaactcac tcacgcgcaa
    34321 gacatcgacc agcttgtgta attgcttttc gatctgctca agtacttttt catcgcccac
    34381 ggtctggatg gtcatacgcg ataatgtcgg atcgtcggtt ggcgcaacgg tcaggctttc
    34441 aatgttgtag ccacgctggg aaaaaaggcc aatcacgcgg gataacgcgc ctgattcatt
    34501 ttcgagtaag actgataata tccggcgcat aatcaggttc tctccgtttt gcttaaccac
    34561 atttcatcca ttccgccccc gcgaatctgc atcgggtaga cgtgctcgct gccatcgacg
    34621 gtaacatcaa caaacaccag gcgattattg cgcacctgtt ccagcgcctc gctaagtttg
    34681 ctttccagct catgcggatg agaaatctgg atcccgacat gcccataggc ttccgccaga
    34741 cggacgaaat cgggtagcga ttgcatataa gattgtgaat gacggccgga atagatcatg
    34801 tcctgccact gcttcaccat ccccagatag cggttattga gattcaccac cagtacgggc
    34861 aactcgtatt gcaacgcggt agacagttcc tggatgttca tctgaatact gccgtcgcca
    34921 gtgacgcaaa ccacggtttc ttctggcaac gccattttga cgcccagtgc cgcaggtaaa
    34981 ccaaaaccca tcgtgccgag gccaccggaa ttgatccagc gacgcggttt gtcgaatgga
    35041 taataaagtg cagcaaacat ctggtgctgc ccgacatcgg acgtcacgta agcgtctccc
    35101 ttcgtcaacc gccaaagagt ctcgatcacc gcctgcggtt taatcttttc actgtgagtg
    35161 tcatatttca ggcactgacg agcgcgccac tgttcaattt gctgccacca gtcgcggatc
    35221 tcatccagtg gttgatgggc ggattcttgc gacaagagtt caagcatttg ttcgaggacc
    35281 tggcgagcat cccccacaat cgggatatcc gcagtcacgg ttttagaaat ggaagtagga
    35341 tcaatatcga tatgcagaac agtggcattt gggcagtact ttgccagatt gttcgtcgtt
    35401 cggtcatcaa atcgtacccc gacggcgaaa atcacatccg cgttatgcat cgtcatattg
    35461 gcttcgtagg taccgtgcat tcccagcatg cccagtgcct gacgatgcgt tgccggaaac
    35521 gcccccagcc ccatcaatga gcaaacaacg ggcagattca acgcctccac cgtttctttc
    35581 aactgctgat ggcagcccgc cgtgattgcc ccaccgccta cgtagacaac cggttttttt
    35641 gccgctacca gcgtttgcag agcacgctta atttgccctt tatgtccggt agtagtggga
    35701 ttgtaagaac gcatactgac cgactccggc cagacatagg gtaatttgtt cgccggatta
    35761 agaatatctt tcggtaaatc aacgactact ggtcctgggc gaccacttgc cgccagccag
    35821 aaagcctttt tcagcacctg cggaatgtct tccgtttgct taaccagaaa actgtgttta
    35881 accaccggtc gcgaaatccc caccatgtcg cactcctgaa aggcatcgta acctatcaac
    35941 gaggtcgcta cctgcccgga aaggacaact aatggaatgg aatccatata agcggtggcg
    36001 atgccagtaa tcgcattggt cgcccctgga cccgacgtta ccagcacgac gccgacttcc
    36061 ccggtcgcgc gcgccaggcc atcggccata tgcaccgccg cctgctcatg acgaactaat
    36121 acatgatcaa taccacccac ggtatgcaat gcatcataaa tatcaaggac tgcgcctccg
    36181 ggataaccga atacttgttt aacgccctga tcgataagcg atcggacgac catctcggct
    36241 ccagacaaca tctccatggc ctgcctcact gtttgacgga aaaaatgtgt aaaacacatt
    36301 aaccgtccga tcttgcttaa gcaattggca gccagagaca taaacacggg gtaataagaa
    36361 taaacaggag gaaaggtgaa aagaaacgat aaaatcctcc attacagagg attaaattga
    36421 taataaatcg gctgaatccc acaacttaca gcaatttctc tattttttta attaatccta
    36481 aaatagagag cccacacatt caggataaaa ttctacattc aataaaaaat gcagaataaa
    36541 ccagacattc atgtctgacc tattctgcaa tcagttagcg tttgcaaatt gagactaatt
    36601 gctcttccat ccactgatgg cctttatcgc gcccggcagc ttcatgccag gagagataac
    36661 aggttctgct gttttgtttt aacggcagcg gtaatacctg taattctaag gattcagcga
    36721 actcttcagc cagccaacgc ggcgcaatag cgaccaaatg cgtttgcgac accacgctaa
    36781 gtacgctcat cattgccatg ccctgatacg cgatactggc ttgcttatct accgtgtcat
    36841 accaaggttg actaaatgac gcgaaacgat cgagcgaaac cgccgcatgt tgttcgttat
    36901 aaacatcatg tttcagtaac gggcccttaa ttgttggatg atttttgctg gctaccagca
    36961 ccatttcatc tttaaataat ggtacgctgg taaattcagg acgatggaag tcttcataac
    37021 taatcacaaa ctccgtttcc tgataacgca gctgatgttc agtgttctga tttaatgaag
    37081 acttgaacat aacatgtata tttggcgcaa tctgctcaat gtgattataa atctgcgagg
    37141 tcagaatgct gtctaacggg ctgcaaacac aaagatgaaa tacacgttca ctgctcgcgg
    37201 gttcaaaacc tgaaccaggc aattcatttt gtactagttg caatgcctga cgaactgaac
    37261 caaaaagttg aaatgcgcga gcagtcggtt gaataccacg gccataacga acaaaaagct
    37321 cgtcattaaa catcaccttc aggcgtgcaa cagcgttact gaccgcaggt tgcgacattc
    37381 ccagaacatg agcggcacga gtaatgtttt gctcctgcat cacggcatcg aaaacggtta
    37441 ataagttgag atcgaccatg cgtagctgtg gcttgcttaa ctccgccgtc tctggatgat
    37501 ctgtttgtac ctctggcata cttaactccc tttcccttat tggaatgcag agaaataatc
    37561 ctcgagaata tgatttatca tgcatataaa ataagaaaaa ggaaaatgag taaaattagg
    37621 aaaacataaa gatacgggtt ctgataaaaa cagaatccat tgccgtggaa tgagtcattt
    37681 accacagcat aataatccat aatgtttttg ttgcgaaaac aatctaatca taactaccgc
    37741 gaatactcaa tcatctacaa aatggatta
//
LOCUS       DAOFQD010000034        36749 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.034, whole genome shotgun sequence.
ACCESSION   DAOFQD010000034 DAOFQD010000000
VERSION     DAOFQD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.034"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            176..871
                     /gene="araD"
                     /locus_tag="QBF46_001790"
     CDS             176..871
                     /gene="araD"
                     /locus_tag="QBF46_001790"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDQ6673979.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGAIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            946..3297
                     /gene="polB"
                     /locus_tag="QBF46_001791"
     CDS             946..3297
                     /gene="polB"
                     /locus_tag="QBF46_001791"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDQ6673980.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYIRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            3462..6368
                     /gene="rapA"
                     /locus_tag="QBF46_001792"
     CDS             3462..6368
                     /gene="rapA"
                     /locus_tag="QBF46_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDQ6673981.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            6380..7039
                     /gene="rluA"
                     /locus_tag="QBF46_001793"
     CDS             6380..7039
                     /gene="rluA"
                     /locus_tag="QBF46_001793"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDQ6673982.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(7156..7971)
                     /gene="djlA"
                     /locus_tag="QBF46_001794"
     CDS             complement(7156..7971)
                     /gene="djlA"
                     /locus_tag="QBF46_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDQ6673983.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            8226..10580
                     /gene="lptD"
                     /locus_tag="QBF46_001795"
     CDS             8226..10580
                     /gene="lptD"
                     /locus_tag="QBF46_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDQ6673984.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            10633..11919
                     /gene="surA"
                     /locus_tag="QBF46_001796"
     CDS             10633..11919
                     /gene="surA"
                     /locus_tag="QBF46_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDQ6673985.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11919..12908
                     /gene="pdxA"
                     /locus_tag="QBF46_001797"
     CDS             11919..12908
                     /gene="pdxA"
                     /locus_tag="QBF46_001797"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDQ6673986.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSRAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            12905..13726
                     /gene="rsmA"
                     /locus_tag="QBF46_001798"
     CDS             12905..13726
                     /gene="rsmA"
                     /locus_tag="QBF46_001798"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDQ6673987.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQRGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            13729..14106
                     /gene="apaG"
                     /locus_tag="QBF46_001799"
     CDS             13729..14106
                     /gene="apaG"
                     /locus_tag="QBF46_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDQ6673988.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            14113..14961
                     /gene="apaH"
                     /locus_tag="QBF46_001800"
     CDS             14113..14961
                     /gene="apaH"
                     /locus_tag="QBF46_001800"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDQ6673989.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAVAS"
     gene            complement(14958..15272)
                     /locus_tag="QBF46_001801"
     CDS             complement(14958..15272)
                     /locus_tag="QBF46_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HDQ6673990.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(15275..15508)
                     /locus_tag="QBF46_001802"
     CDS             complement(15275..15508)
                     /locus_tag="QBF46_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HDQ6673991.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(15594..16073)
                     /gene="folA"
                     /locus_tag="QBF46_001803"
     CDS             complement(15594..16073)
                     /gene="folA"
                     /locus_tag="QBF46_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDQ6673992.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLAKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(16265..18127)
                     /gene="kefC"
                     /locus_tag="QBF46_001804"
     CDS             complement(16265..18127)
                     /gene="kefC"
                     /locus_tag="QBF46_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDQ6673993.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATSILLVILNRLEQSSTKEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKIGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(18120..18650)
                     /gene="kefF"
                     /locus_tag="QBF46_001805"
     CDS             complement(18120..18650)
                     /gene="kefF"
                     /locus_tag="QBF46_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDQ6673994.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLDGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(18761..20092)
                     /locus_tag="QBF46_001806"
     CDS             complement(18761..20092)
                     /locus_tag="QBF46_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6673995.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(20150..20437)
                     /gene="fixX"
                     /locus_tag="QBF46_001807"
     CDS             complement(20150..20437)
                     /gene="fixX"
                     /locus_tag="QBF46_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDQ6673996.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(20434..21720)
                     /locus_tag="QBF46_001808"
     CDS             complement(20434..21720)
                     /locus_tag="QBF46_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDQ6673997.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGTACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(21771..22712)
                     /locus_tag="QBF46_001809"
     CDS             complement(21771..22712)
                     /locus_tag="QBF46_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDQ6673998.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(22727..23497)
                     /locus_tag="QBF46_001810"
     CDS             complement(22727..23497)
                     /locus_tag="QBF46_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDQ6673999.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            23971..25485
                     /gene="caiT"
                     /locus_tag="QBF46_001811"
     CDS             23971..25485
                     /gene="caiT"
                     /locus_tag="QBF46_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDQ6674000.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            25516..26658
                     /gene="caiA"
                     /locus_tag="QBF46_001812"
     CDS             25516..26658
                     /gene="caiA"
                     /locus_tag="QBF46_001812"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDQ6674001.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            26787..28004
                     /gene="caiB"
                     /locus_tag="QBF46_001813"
     CDS             26787..28004
                     /gene="caiB"
                     /locus_tag="QBF46_001813"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDQ6674002.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDDWLATHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            28078..29631
                     /gene="caiC"
                     /locus_tag="QBF46_001814"
     CDS             28078..29631
                     /gene="caiC"
                     /locus_tag="QBF46_001814"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDQ6674003.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNCPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            29740..30525
                     /gene="caiD"
                     /locus_tag="QBF46_001815"
     CDS             29740..30525
                     /gene="caiD"
                     /locus_tag="QBF46_001815"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDQ6674004.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGVVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            30531..31121
                     /gene="caiE"
                     /locus_tag="QBF46_001816"
     CDS             30531..31121
                     /gene="caiE"
                     /locus_tag="QBF46_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDQ6674005.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(31330..31725)
                     /gene="caiF"
                     /locus_tag="QBF46_001817"
     CDS             complement(31330..31725)
                     /gene="caiF"
                     /locus_tag="QBF46_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDQ6674006.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(31986..35207)
                     /gene="carB"
                     /locus_tag="QBF46_001818"
     CDS             complement(31986..35207)
                     /gene="carB"
                     /locus_tag="QBF46_001818"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDQ6674007.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(35225..36373)
                     /gene="carA"
                     /locus_tag="QBF46_001819"
     CDS             complement(35225..36373)
                     /gene="carA"
                     /locus_tag="QBF46_001819"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDQ6674008.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGMPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         8832 a         9902 c         9590 g         8425 t
ORIGIN      
        1 ctgatgcgac gctggcgcgt cttatcaggc ctacgcgttc cgattttgta ggccggataa
       61 gcaaagcgca tccggcattc aacgcctgat gcgacgctgg cgcgtcttat caggcctacg
      121 cgctgcgatt ttgtagaccg gataagcgaa gcgcatccgg cacgaaggag tcaacatgtt
      181 agaagatctc aaacgccagg tattagaagc caacctggcg ctgccaaaac acaacctggt
      241 cacgctcacc tggggcaacg tcagcgccgt tgatcgcgag cgcggcgtct ttgtgatcaa
      301 accttccggc gtcgattaca gcgtcatgac cgctgacgat atggtcgtgg ttagcatcga
      361 aaccggtgaa gtggttgaag gtacgaaaaa gccctcttcc gacacgccaa ctcaccggtt
      421 gctctatcag gcattcccgt ccattggcgg cattgtgcac acccactcgc gccacgccac
      481 catctgggcg caggcgggtc agtcaatccc ggcgactggt accacccacg ccgactattt
      541 ctacggcgcc attccctgca cccgcaaaat gaccgacgca gaaatcaacg gcgaatatga
      601 gtgggaaacc ggtaacgtca tcgtagaaac cttcgaaaaa cagggtatcg atgcagcgca
      661 aatgcccggc gtcctggtcc attctcacgg cccatttgca tggggcaaaa atgccgaaga
      721 tgcggtgcat aacgccatcg tgctggaaga ggtcgcttat atggggatat tctgccgtca
      781 gttagcgccg cagttaccgg atatgcagca aacgctgctg gataaacact atctgcgtaa
      841 gcatggcgcg aaggcgtatt acgggcagta atgactgtat aaaaccacag ccagtcaaac
      901 gaaaccaggc tatactcaag cctggttttt tattggattt tcagcgtggc gcaggcaggt
      961 tttatcttaa cccgacactg gcgggacacc ccgcaaggga ccgaagtttc tttctggctg
     1021 gcgacggaca acgggccgtt gcaggtcacg cttgcaccgc aagagtccgt ggcgtttatt
     1081 cccgccgatc aggttccccg cgctcagcat attttgcagg gtgaacaagg ctttcgcctg
     1141 acaccgctgg cgttaaagga ttttcaccgc cagccagtgt atggccttta ctgtcgagcc
     1201 catcgccagt tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac cgtctacgag
     1261 gccgatgtgc gcccgccaga acgctatctg atggagcggt ttatcacctc accggtgtgg
     1321 gtcgagggtg atatgcacaa tggcactatc gttaatgccc gtctgaaacc gcatcccgac
     1381 tatcgtccgc cgctcaagtg ggtttctata gatattgaaa ccacccgcca cggtgagctg
     1441 tactgcatcg gcctggaagg ctgcgggcag cgcatcgttt atatgctggg gccggagaat
     1501 ggcgacgcct cctcgcttga tttcgaactg gaatacgtcg ccagccgccc gcagttgctg
     1561 gaaaaactca acgcctggtt tgccaactac gatcctgatg tgatcatcgg ttggaacgtg
     1621 gtgcagttcg atctgcgaat gctgcaaaaa catgccgagc gttaccgtct tccgctgcgt
     1681 cttgggcgcg ataatagcga gctggagtgg cgcgagcacg gctttaaaaa cggcgtcttt
     1741 tttgcccagg ctaaaggtcg gctaattatc gacggtatcg aggcgctgaa atccgcgttc
     1801 tggaatttct cttcattctc gctggaaact gtcgctcagg agctattagg cgaaggaaaa
     1861 tctatcgata acccgtggga tcgaatggac gaaattgacc gccgtttcgc cgaagataaa
     1921 cctgcgctgg caacttataa cctgaaagat tgcgagctgg tgacgcagat cttccacaaa
     1981 actgaaatca tgccattttt actcgaacgg gcaacggtga acggcctgcc ggtggaccga
     2041 cacggcggtt cggtggcggc atttggtcat ctctattttc cgcgaatgca tcgcgctggt
     2101 tatgtcgcgc ctaatctcgg cgaagtgccg ccgcacgcca gccctggcgg ctacgtgatg
     2161 gattcacggc cagggcttta tgattcagtg ctggtgctgg actataaaag cctgtacccg
     2221 tcgatcatcc gcacctttct gattgatccc gtcgggctgg tggaaggcat ggcgcagcct
     2281 gatccagagc acagtaccga aggttttctc gatgcctggt tctcgcgaga aaaacattgc
     2341 ctgccggaga ttgtgactaa catctggcac gggcgcgatg aagccaaacg ccagggtaac
     2401 aaaccgctgt cgcaggcgct gaaaatcatc atgaatgcct tttatggcgt gctcggcacc
     2461 accgcctgcc gcttcttcga tccgcggctg gcatcgtcga tcaccatgcg tggtcatcag
     2521 atcatgcggc aaaccaaagc gttgattgaa gcacagggct acgacgttat ctacggcgat
     2581 accgactcaa cgtttgtctg gctgaaaggc gcacattcgg aagaagaagc ggcgaaaatc
     2641 ggtcgtgcac tggtgcagca cgttaacgcc tggtgggcgg aaacgctgca aaaacaacgg
     2701 ctgaccagcg cattagaact ggagtatgaa acccatttct gccgttttct gatgccaacc
     2761 attcgcggag ccgataccgg cagtaaaaag cgttatgccg gactgattca ggagggcgac
     2821 aagcagcgga tggtgtttaa agggctggaa accgtgcgca ccgactggac gccgctggcc
     2881 cagcagtttc agcaggagct atacctgcgt atcttccgta acgagccata tcaggaatac
     2941 atccgcgaaa ccatcgacaa actgatggcg ggtgaactgg atgcgcggct ggtttaccgt
     3001 aaacgccttc gccgtccgct gagcgaatat cagcgtaatg ttccgcctca tgtacgcgcc
     3061 gctcgccttg ccgatgaaga aaaccaaaag cgaggtcgcc ccttgcaata ccagaaccgc
     3121 ggcaccatta agtacgtatg gaccaccaac ggcccggagc cgctggacta ccaacgttca
     3181 ccactggatt acgaacacta tctgacccgc cagctacaac ccgtggcgga gggaatactt
     3241 ccttttattg aggataattt tgctacactt atgaccgggc aacttgggct attttgagca
     3301 aaaaaaagag ttcgccagat accattttga tgcgtgacga atgctttgcc atccagtacc
     3361 atagcgccct ttccattcct ggacctgaat aacaccacta cctcataagc acggtagcgg
     3421 gtggttattg cctgcaatta aagatataga gccgaacaca tatgcctttt acacttggtc
     3481 aacgctggat cagcgataca gaaagcgaat tgggacttgg aaccgttgtc gcggtggatg
     3541 cgcgaactgt cactttactt ttcccatcta ctggtgaaaa ccgtctgtac gcacgcagtg
     3601 attcccccgt gacccgcgtg atgttcaacc ctggtgatac tattaccagc catgacggct
     3661 ggcagatgca agtcgaagaa gtaaaagaag aaaatggctt gctgacctat atcggtactc
     3721 gcctggatac tgaagagtcc ggcgtagccc tgcgtgaagt tttccttgat agcaaactgg
     3781 tgttcagcaa accgcaggac cgtctgtttg ccgggcagat tgaccgtatg gaccgctttg
     3841 cgctgcgtta tcgcgcgcgt aaatattcca gcgaacagtt ccgtatgccg tacagcggcc
     3901 tgcgcggtca gcgtaccagc ctgatcccgc atcagctcaa catcgctcat gatgttggtc
     3961 gccgccacgc gccgcgcgtc ctgctggctg acgaagtggg tttagggaaa accattgaag
     4021 ccgggatgat cctgcatcag caactgctct ctggcgctgc tgaacgtgtg ctaattatcg
     4081 tcccggaaac cttacagcat cagtggctgg tagaaatgct gcgccgtttc aacctgcgct
     4141 ttgcgctatt tgatgatgag cgttatgccg aagctcagca cgatgcttac aacccgtttg
     4201 acaccgaaca gctggtgatt tgctcgctgg attttgcccg tcgtagcaaa cagcgcctgg
     4261 aacatctctg tgaagccgaa tgggacctgc tggtggtcga tgaagcgcat cacctggtgt
     4321 ggagcgaaga tgcgccaagc cgtgaatatc aggccattga acaactggca gagcacgtgc
     4381 cgggcgttct gctgctgacc gcgaccccgg aacagctggg gatggaaagc cacttcgccc
     4441 gtctgcgtct gctggacccg aaccgtttcc acgattttgc gcagttcgtt gaagagcaga
     4501 aaaattatcg tccggttgcg gacgccgttg ccatgctgct ggcaggtaac aaactgagca
     4561 atgacgaact gaacatgctc ggcgagatga tcggcgagca ggatatcgag ccgctgttgc
     4621 aggcagcaaa cagcgacagc gaagatgccc agagcgcccg tcaggagctg gtttcgatgc
     4681 tgatggatcg ccacggcacc agccgcgtgc tgttccgtaa cacgcgtaac ggtgtgaaag
     4741 gattcccgaa acgcgagctg cacaccatta agctgccgct accgacgcag tatcagacgg
     4801 ctattaaagt ctccggcatt atgggcgcac gtaaaagtgc ggaagatcgt gctcgcgata
     4861 tgctctaccc ggagcgtatt tatcaggaat ttgaaggtga taacgccacc tggtggaact
     4921 tcgatccgcg cgttgagtgg ctgatgggct acctgaccag ccatcgctct cagaaagtgc
     4981 tggtgatctg cgccaaagct gccactgcgc tgcaactgga gcaggtactg cgcgaacgtg
     5041 aaggtattcg cgctgcggtg ttccacgaag gtatgtcgat tatcgaacgt gaccgcgctg
     5101 ccgcctggtt tgccgaagaa gacaccggcg cacaggtact gctgtgctca gaaatcggtt
     5161 ctgaaggacg taacttccag ttcgccagcc acatggtgat gtttgacctg ccattcaacc
     5221 cggatctact ggagcagcgt attggtcgtc tggatcgtat cggccaggcg cacgatattc
     5281 agatccatgt gccttatctg gagaaaaccg ctcagtcggt gctggtgcgc tggtatcacg
     5341 aaggtctgga tgcatttgag cacacctgcc cgaccggacg cactatttac gatagcgtat
     5401 acaacgatct gattaactat ctggcttcac cggatcaaac cgaaggcttt gacgatctga
     5461 tcaaaaactg ccgcgagcaa catgaagcgc tgaaagcaca gctggaacag ggtcgtgacc
     5521 gcctgctgga aatccactcc aacggtggcg aaaaagccca ggcactggca gaaagcattg
     5581 aagagcagga tgacgatacc aacctgatcg ccttcgccat gaacctgttc gatattatcg
     5641 gtatcaatca ggacgatcgc ggcgacaaca tgatcgtgct gacgccgtcc gatcatatgc
     5701 tggtgccgga cttccctggc ctgtcggaag atggcatcac catcaccttt gatcgtgaag
     5761 tggcgctggc gcgtgaagat gcacagttta ttacctggga gcatccgctg atccgcaacg
     5821 gtctggatct gatcctttct ggcgataccg gtagcagcac gatttcactg ttaaaaaaca
     5881 aagcgttgcc ggtaggtacg ctgttggtgg aactgattta tgtggttgaa gcccaggctc
     5941 cgaagcagtt gcagctcaac cgcttcctgc caccgacgcc ggtacgtatg ctgctggata
     6001 aaaacggcaa caacctggcg gcgcaggtag agtttgaaac ctttaaccgc cagcttaacg
     6061 cggttaaccg tcacaccggc agcaaactgg ttaacgccgt gcagcaggat gttcacgcta
     6121 tccttcaact gggtgaagcg cagatcgaga aatctgcccg tgcattgatt gatgcagcgc
     6181 gtaacgaagc cgacgaaaaa ctgtctgccg agctgtctcg tctggaagct ctgcgtgcag
     6241 tgaacccgaa cattcgtgac gacgaactga ccgccattga gagcaaccgt cagcaggtaa
     6301 tggaaagcct ggatcaggca ggttggcgtc tggatgccct gcgtttgatc gttgtaacgc
     6361 atcagtaacg gagccgaaaa tggggatgga aaactacaat ccaccgcagg aaccctggtt
     6421 ggttatcctg tatcaggatg accatattat ggtggtcaac aagccgagcg gtttgttgtc
     6481 agtgccgggt cgtctggaag agcacaaaga cagcgtgatg acgcgcattc agcgtgatta
     6541 tccgcaggca gaatcggtgc atcgtctgga tatggctacc agcggcgtga ttgtagtggc
     6601 gctgaccaaa gccgcggagc gggagttaaa acgccagttc cgcgagcgcg agccgaaaaa
     6661 gcagtatgtg gcccgcgtct gggggcatcc atcccccgca gaaggtctgg tggatctgcc
     6721 gctgatttgc gactggccaa accgcccgaa acagaaagtc tgttacgaaa cgggtaaacc
     6781 tgcgcagacg gaatatgaag tggtggagta tgcggcggat aacacggcaa gagtggtgtt
     6841 aaaaccgatt accgggcgtt cgcatcaatt gcgtgtgcat atgctggcgc tggggcatcc
     6901 gattctcggc gatcgttttt atgcatcacc agaagcgaga gcgatggcac cgcgtttgtt
     6961 gctacatgca gagatgttga cgattaccca tccggcgtat ggcaatagta tgacgtttaa
     7021 agcgccagcg gatttttaat cgtcacgctg gcgcagttcg gtaggcggat aaggcgttca
     7081 cgccgcatcc ggcaattgtg cgccgattgc ctgatgcgac gcgggccgcg tcttatcagg
     7141 cctacagttt aaaacttatt taaacccttt ctgctgcttt atcagctcat atgcctgctg
     7201 aatttcctgc gctttctgct tcgccatctc catcatctca ggcggcaaac ctttcgccac
     7261 cagcttatcg ggatggtgtt cactcatcag cttacggtag gcacgtttga tggtggtcgc
     7321 atcatccgtc ggcttcacgc ccagcacatt acaggcatct tccagcgttg ggccacgctg
     7381 cgcttgctgc cagttaccac cgccagtttg ctgctgataa ccgccgccaa actgtgcacc
     7441 gccctgcatc atgcgcaaaa actggtcaaa ctgagcgcgg gagatcccta attcttctgc
     7501 aatgacatac agcaccgccc gttcattcgg gtgcagtgaa ccatcagcaa acgccgcctg
     7561 aatctggatc tccagaaaca tacgaattaa gtcaaaacga ccaaagcaga cactgcgaaa
     7621 ctggcgcatc ttttcgcgca gcgggtaatt gtctgatttt cccacccgga acgcattttg
     7681 cgccgcagta cgggaagcgc catgaagatt cattcggtcc atcaactggc tggcgatatg
     7741 aatatcagcc tccgtgacgc gacctttgga tttggttaaa tgccccatca cttcaaaagt
     7801 ggtggcaaaa aacagcgcct gacgctcacg ctggttggcg aaccacgcca ttttacggct
     7861 acgggcttta tcaaacatat ggccaattaa caggcctaac actacgcccc aaaagccgcc
     7921 gcccatcagt aaggccacgg ccacgccaat gatttttccc caatactgca tatattcccc
     7981 aaatcgacac acggatatca gggctatctc ccacaatata aaggtgcttt taccgttttc
     8041 cggctgcggt caattgtggg acatcgccta taatttgcat tatcatacct gtcattcaat
     8101 gccgtgccta acaccactga cgctattcgg acaggattaa cactagcgta gagatgacga
     8161 gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc gatgatggaa caataaaatc
     8221 aacgtatgaa aaaacgtatc cccactctcc tggccaccat gattgccacc gccctttata
     8281 gtcaacaggg actggcagcc gacctcgcct cacagtgcat gttgggcgtg ccaagctatg
     8341 accgtcctct ggtacagggc gataccaatg acttacccgt gactatcaat gctgaccacg
     8401 cgaaagggga ctacccggat gacgccgtgt ttactggcag cgtggatatc atgcagggta
     8461 acagccgtct gcaggccgac gaagtgcagc tccatcaaaa agaggcacca ggacaaccgg
     8521 agccggtacg taccgttgat gcgctcggta atgtccatta cgacgataac caggtgatcc
     8581 tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga taccaacgtc tgggaaggtg
     8641 attaccagat ggtgggtcgc cagggtcgcg gtaaagcgga cctgatgaaa caacgtggcg
     8701 aaaaccgcta taccattctg gataacggta gctttacctc ctgtctgccg ggttctgaca
     8761 cctggagcgt ggtaggtagc gaaattattc atgaccgcga agaacaagtt gcggagatct
     8821 ggaacgcccg ctttaaggtg ggtccggtac cgatctttta tagcccctat ttgcagttgc
     8881 cggtgggtga caaacgtcgc tctggtttct tgatcccgaa cgccaagtac accaccacca
     8941 actactttga gttctacctg ccatattact ggaacatcgc gccaaatatg gatgccacca
     9001 tcacgccgca ttatatgcat cgtcgtggca acatcatgtg ggagaacgaa ttccgctacc
     9061 tctcccaggc gggcgctggc ttgatggaac tggactatct gccttcagat aaagtctatg
     9121 aagatgaaca cccgaacgat gacagttcac gtcgttggtt attctactgg aaccactccg
     9181 gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac caaggtcagc gatcctagct
     9241 acttcaatga tttcgataac aagtacggtt ccagtactga cggctacgca acgcaaaaat
     9301 tcagcgttgg ctatgcggtg caaaacttca atgccaccgt ttcaaccaag cagttccagg
     9361 ttttcagcga acagaacacc agtagctact cggcagagcc gcagttagac gttaattact
     9421 accagaatga tgttggtccg tttgatacgc gtatttacgg ccaggcagtg cactttgtta
     9481 acaccagaga cgacatgcct gaagcaaccc gtgttcacct ggaaccgacc atcaatttgc
     9541 cgctctctaa taactggggc agcatcaata ccgaagcgaa gttgctggca acccattatc
     9601 agcaaaccaa tcttgactgg tataactcca gaaacacgac caagctggac gaatccgtta
     9661 accgcgtaat gccgcaattc aaagttgacg gcaaaatggt ctttgaacgc gatatggaaa
     9721 tgctggctcc gggttatacc caaacgctgg aaccgcgcgc gcagtatttg tacgtgccgt
     9781 atcgcgatca gagcgacatc tataactacg actcgtctct gctgcaatct gactactctg
     9841 gcctgttccg ggaccggact tacggcggtc ttgaccgtat tgcctccgct aaccaggtga
     9901 cgaccggtgt cacatctcgc atatatgatg atgctgccgt tgaacgtttt aatatttccg
     9961 ttggtcaaat ctactatttc acggagtctc gcactggcga tgacaacata acatgggaga
    10021 atgacgacaa aacgggttca ctggtgtggg caggcgatac ttactggcgt atctccgagc
    10081 gttggggatt gcgtggcggg attcagtacg atacacgtct ggataacgta gcgaccagta
    10141 actccagcat tgaataccgt cgggatgaag accgtctggt acagctgaat taccgttacg
    10201 ccagcccgga atatattcag gctacgctgc ctaagtacta ttccactgct gagcaatata
    10261 agaatggtat ttcgcaggta ggtgctgtcg ccagctggcc aattgccgat cgttggtcca
    10321 ttgttggggc ctactactac gacaccaatg ctaacaagca agccgactct atgttaggtg
    10381 tgcaatacag ctcctgctgc tatgcaattc gcgtcggtta cgagcggaag ctgaacggtt
    10441 gggataacga taaacaacat gcggtatatg acaacgcaat cggctttaac atcgaacttc
    10501 gcggcctgag ctccaactac ggtctgggta cgcaagagat gctgcgttcg aacattctgc
    10561 cgtatcaaaa cactttgtga tttgttgatt taccacgtaa tccgcagtgc ggttaattga
    10621 aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg gtatcgccat gatcgcgaat
    10681 accagtttcg ctgcccccca ggtagtcgat aaagtcgcag ccgtcgtcaa taacggcgtc
    10741 gtgctggaaa gcgacgttga tggattaatg cagtcggtaa aactgaacgc tgctcaggca
    10801 aggcagcaac ttcctgatga cgcgacgctg cgccaccaaa tcatggaacg tttgatcatg
    10861 gatcaaatca tcctgcagat ggggcagaaa atgggagtga aaatctccga tgagcagctg
    10921 gatcaggcga ttgctaacat cgcgaaacag aacaacatga cgctggatca gatgcgcagc
    10981 cgtctggctt acgatggact gaactacaac acctatcgta accagatccg caaagagatg
    11041 attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca tcaccatcct gccgcaggaa
    11101 gtcgaatccc tggcgcagca ggtgggtaac caaaacgacg ccagcactga gctgaacctg
    11161 agccacatcc tgatcccgct gccggaaaac ccgacctctg atcaggtgaa cgaagcggaa
    11221 agccaggcgc gcgccattgt cgatcaggcg cgtaacggcg ctgatttcgg taagctggcg
    11281 attgctcatt ctgccgacca gcaggcgctg aacggcggcc agatgggctg gggccgtatt
    11341 caggagttgc ccgggatctt cgcccaggca ttaagcaccg cgaagaaagg cgacattgtt
    11401 ggcccgattc gttccggcgt tggcttccat attctgaaag ttaacgacct gcgcggcgaa
    11461 agcaaaaata tctcggtgac cgaagttcat gctcgccata ttctgctgaa accgtcgccg
    11521 atcatgactg acgaacaggc ccgtgtgaaa ctggaacaga ttgctgctga tatcaagagt
    11581 ggtaaaacga cttttgctgc cgcagcgaaa gagttctctc aggatccagg ctctgctaac
    11641 cagggcggtg atctcggctg ggctacacca gatattttcg atccggcctt ccgtgacgcc
    11701 ctgactcgcc tgaacaaagg tcaaatgagt gcaccggttc actcttcatt cggctggcat
    11761 ttaatcgaac tgctggatac ccgtaatgtc gataaaaccg acgctgcgca gaaagatcgt
    11821 gcataccgca tgctgatgaa ccgtaagttc tcggaagaag cagcaagctg gatgcaggaa
    11881 caacgtgcca gcgcctacgt taaaatcctg agcaactaat ggttaaaacc caacgtgttg
    11941 tgatcactcc cggcgagccc gccgggattg gcccggactt agttgtccag cttgcacagc
    12001 gtgagtggcc ggtcgaactg gttgtttgtg ccgatgccac tctccttacc gaccgggcag
    12061 cgatgctcgg tttgccgctc accctccgcc cttattcccc caactcccct gcacaaccgc
    12121 aaactgcggg cacattaacg ctacttcctg tcgcgctacg tgaatctgtc actgcggggc
    12181 agttagcggt tgaaaatggg cattacgtgg tggagacgct ggcgcgagcg tgcgatggct
    12241 gtctgaacgg tgaatttgct gcgctgatca caggccccgt gcataaaggc gtcattaacg
    12301 acgcaggcat tccgtttacc ggtcataccg agtttttcga agagcgttcg cgggcgaaaa
    12361 aggtggtgat gatgctggcg accgaagaac ttcgcgtggc gctggcaacg acgcatttac
    12421 cgctgcgcga tatcgcagac gctatcaccc ctgcactgtt gcacgaagtg attgctattt
    12481 tgcatcacga tttacggacc aaatttggta ttgccgaacc gcgcattctg gtctgcgggc
    12541 tgaatccgca cgcgggcgaa ggcggtcata tgggtacgga agagatagac accattattc
    12601 cggtgctcaa tgagctgcgg gcgcagggga tgaaactcaa cgggccgctg cctgccgata
    12661 ccctgtttca gccgaaatat ctcgataacg ccgatgccgt gctggcgatg taccacgatc
    12721 agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg tgtgaacatt acgctgggcc
    12781 taccctttat tcgcacatca gtggaccacg gcaccgcgct tgaactggcg ggacgtggca
    12841 aagccgatgt cggcagtttt attacggcgc ttaatctcgc catcaaaatg attgttaaca
    12901 cccaatgaat aatcgagtcc accagggcca cttagcccgt aaacgcttcg ggcaaaactt
    12961 tctcaacgat cagttcgtga tcgacagtat tgtgtctgcc attaaccctc aaaggggcca
    13021 ggcgatggtc gaaatcggcc ccggtctggc ggcattgacc gaaccggtcg gcgaacgtct
    13081 ggaccagctg acggtcatcg aacttgaccg cgatctggcg gcacgtctgc aaacgcatcc
    13141 attcttaggc ccgaaactga cgatttatca gcaggatgcg atgaccttta actttggcga
    13201 actggccgag aaaatgggcc agccgctgcg tgttttcggc aacctgcctt ataacatctc
    13261 cacgccgttg atgttccatc tgtttagcta tactgatgcc attgccgaca tgcactttat
    13321 gttgcaaaaa gaggtggtga atcgtctggt tgcaggaccg aacagcaaag cgtatggtcg
    13381 attaagcgtc atggcgcaat actattgcaa tgtgatcccg gtactggaag taccgccgtc
    13441 agcctttaca ccaccaccca aagtggattc cgccgtcgtg cgcctggttc ctcatgcaac
    13501 gatgcctcac ccggttaaag atgttcgtgt gttgagccgc atcaccaccg aagcctttaa
    13561 ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg tttagcgtcg aggtgttaac
    13621 gggaatgggg atcgacccgg cgatgcgagc ggaaaatatc tctgtcgcgc aatattgcca
    13681 gatggcgaac tatctggcgg agaacgcgcc tttgcaggag agttaacgat gatcaattcg
    13741 ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg ctcaatcttc acctgataat
    13801 gaacgttacg tttttgctta taccgtaacc atacgcaatc tggggcgagc gccagtgcag
    13861 ttgttggggc gttactggct gatcaccaat ggcaatggcc gtgaaaccga agtccagggc
    13921 gaaggagtgg ttggcgtcca gccacttatc gcgcctggcg aagagtacca gtacaccagc
    13981 ggtgcaatca ttgaaacccc gctgggcacc atgcagggtc actacgaaat gatcgatgaa
    14041 aatggcgtcc ctttcagcat cgacattccc gtattccgac tcgccgttcc cacactcatt
    14101 cattaaaaga atatggcgac ataccttatt ggcgacgttc atggttgtta cgatgaactg
    14161 atcgcattgc tgcataaagt agaatttacc cctgggaaag ataccctctg gctgacgggc
    14221 gatctggtcg cgcgcggccc gggttcgctg gatgttctgc gctatgtgaa atccttaggc
    14281 gacagcgtac gtctggtgct gggcaatcac gatctgcatc tgctggcggt atttgccggg
    14341 atcagccgca ataaaccgaa agatcgcctg acaccgctgc tggaagcgcc ggatgccgac
    14401 gagctgctta actggctgcg tcgccagcct ctgctgcaaa tcgacgaaga gaaaaagctg
    14461 gtgatggccc acgcagggat cacgccgcag tgggatctgc agaccgccaa agagtgcgcg
    14521 cgtgatgtag aagcggtgct gtcgagcgac tcctatccct tctttctcga cgctatgtac
    14581 ggcgatatgc caaataactg gtcaccggaa ttgcgggggc tgggaagact gcgttttatc
    14641 accaacgcct ttacccgtat gcgtttttgc ttcccgaacg gtcaactgga tatgtacagc
    14701 aaagaatcgc cggaagaggc ccctgcccca ctgaaaccgt ggtttgcgat tcctggccct
    14761 gtcgctgaag aatacagcat cgcctttggt cactgggcat cgctggaagg caaaggtacg
    14821 ccagaaggca tttatgcgct ggataccggc tgctgctggg gcggttcatt aacctgcctg
    14881 cgctgggaag ataaacagta ttttgtccag ccgtcgaaca gacataagga tatgggcgaa
    14941 ggagaggcgg tagcgtctta aatcccgtcg agcataaaat ctaatgctcc tttaatcaac
    15001 gttcggtgcg cgctggcatc gcaaaatttc gttcccagcg cgttaactgg aacagttgca
    15061 gtctcatgcg tcatgagcac atattctttg ccgtctacca gtaaaaggat tgggttaagc
    15121 cgttctggtg ggcgaatacg gctaaatcgt tcaataggtg ttaatggaat aacaatacgg
    15181 cgattaatct caccaataat gtcgctggta acatcgataa caaagggaaa cgcggcgttc
    15241 ctgctgcgac tgcggtatac cgtaaattgc atcgctagaa gctccggtac tcatcagaga
    15301 aacatccggt ttcatcagcc aattgattta acgcagcgat agcttctgcg ttctcttcac
    15361 gccaacgtgt tgctgcatgt tttttaagtt cagcattgag cgcaaccgta agggtggcgc
    15421 tcatatttaa gcctgcatca cgagcgcgat tgactaactc acggtcgacg gtgacggtca
    15481 cactttgtgt ggtacgtttt gcagtcatga tatcgtgctc ctgtacaatt tatacgtcta
    15541 gtatacacgg atatctgcac gtatcagtga tgccggaatt ctaatataca aaattaccgc
    15601 cgctccagaa tctcaaagca ataactatga gagttctgcg catcagcgtc gtggaattcg
    15661 ctgaataccg attcccagtc atccggctcg taatccggga aatgggtgtc gccttccact
    15721 tctgcgtcga tatgcgtcag atacagtttt tgcgcttttg ccaggaactg ctcataaacg
    15781 cgaccgccgc caatcaccat gatttctggt acgtcaccac acgccgcgat ggcttcatcc
    15841 accgacttca cccacgttac gcgatcgtcc gtacccggtt gactgctgag gataatgttt
    15901 ttgcgtcctg gcagcggacg accgatggat tcccaggtat ggcggcccat aatcacgggt
    15961 ttatttaagg tgttgcgttt aaaccaggcg agatcggcag gcaggttcca cggcatggcg
    16021 ttttccatgc cgataacgcg atctaccgct aacgccgcaa tcagactgat cattgagatt
    16081 tcccgataaa aaaaattgtc gccactatac gtaaagcgta aaccgtcgtc gactggtgcg
    16141 aggatgatgt tgaggaaaat tttatattct gctggcgagt ccacgctctc cccctggact
    16201 cgccgcatta caatgaaaca aaaacaaaca gttagctgta aagtgtgatt tacgtcactc
    16261 tttattagga tgagggtttc gtttccggtt catccgccat gttgccggta tgtttacctt
    16321 cttctgttcc ctgccagcca tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtga
    16381 tgatctcact taacatcgcg ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcat
    16441 tctcaaccat tgccatctct tccaccatct gaatattaaa gcggcggaac acatcggcac
    16501 gttctcgcgc ttcatacggc cccagaccta aactttccag cgccagacgc ccgattttca
    16561 gcgcaccttc gaaggtttca cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt
    16621 agtggtcgac atcgcgggcg cgggcaataa tctgcaaatg cgggaaatgt tctttcacca
    16681 tctccgtcag ttgcaggttg gtttgcggat cgtcaatagc attaatcagc acttccgctt
    16741 tcgccgctcc ggcagattcc agtaaatcca tccgcgtggc atcgccgtaa aacactttca
    16801 taccaaattt acgcagcgtt tcgatatggt ccggatcgtg atcgagcacc accattttca
    16861 ccccgctgga gagcagtaaa cgtccggtaa tctgtccaaa acgaccgaat ccggcaatga
    16921 ttacccgcgg ctgttcttcg tcgatctcat cggcttcacg cgcttcctta gtagaagatt
    16981 gctcaaggcg attgaggatc accagcagaa tcgacgttgc tgccatcgac agcgccaccg
    17041 ccagggtcag cgatttcgcc cactccggct ccagcacatt cgccatctgc gccgcgccaa
    17101 ataccacaaa ggcaaactca ctgccctgcc ctaacaacac cgcaaaccag cgacgctgtt
    17161 tatttggcac ctgcaacggt cgggcaatca gccacagcat ggcgattttg atgatgagga
    17221 aaccgagcag caaaatgaca atgcgcaaag ggttttcaat cagcgtgcca aagtctatcg
    17281 acatgccaac accgatgaaa aacagcccca acagcaaacc tttaaatggc tcgatatcgc
    17341 tttccagcgc atgacggtat tccgagcttg ccagcagtac gcccgccaga aatgcgccca
    17401 tcgccatcga caagccgacc tcttccagca gcaaaccaaa gccaaacacg aggaataaag
    17461 ccacggcact aaacacttcc cgcaagccag agcgggcaac aaaacgcagc gccggacgcg
    17521 tgacatagcg ccccagcaat accaccagta ccagcgcacc cgccactttt aacgccgaga
    17581 gagcaaatgc gcccatcgtc gtcgaggcac tgctcgccgc cagtagcgga atcatcgcca
    17641 ccaacgggat cgccgcgata tcctggaaca gcagcaccgc aaaggcgctg cgacccattt
    17701 gcgtcaccat cagattgcgt tcattcatcg cctgcatggc aatcgccgta gaggagagcg
    17761 ccagcgtcat gccgatcaat tccgcgacct gccagcgcag cccaagtaac atgcagaaca
    17821 gccccagcag gccgccgcaa atcaccatct gcaatgcgcc accgccgaac actgccgcac
    17881 gcagcttcca cagcctttgt ggatcgagtt cgaggccgat aataaacagc atcagcacca
    17941 ccccaatctc ggcaaagtgc agaatagatt cggcatcggt caccagtcgc agcccccacg
    18001 ggccaataat gcagccggcg atcaggtagc caagtaccga tcccagacca agacgtaccg
    18061 caatgggtac aatcagcgcc gccgatccga ggtagatcag cgcctgaacc agcgtatgac
    18121 tatccatgat gggcctcctg ccattccagc agacgttgct tatagtgacg cgcctgccct
    18181 tcgagggttt cgtcgtcaca aataaaggtg cagtgcatgg caaacggtgg cagccagttc
    18241 agcccgcagt agattgccgt cgcctgtagc ggttgcgaca gcacatcaaa gcccggatgc
    18301 gcaccaattt caaaatggct ttccccgccg ccggtcgtca ccgcccacag caaatgtttg
    18361 ccatgcagcg ccgtgccgcc gtgaccgtaa gcccagccgt gcgagaagac tttatcgatc
    18421 caaagtttaa ggagcggagg aatgctgtac cactgcatcg ggtgctgcca gacgatcaga
    18481 tcggcgcgag acagcgcctc ctgctcggcg gcaatatcga tattgaagtc aggatagagt
    18541 tgataaagag agcgaatttc gacgccatcc agcgtccttg cctgttcaag catccgttta
    18601 ttcgcatggg aatgatgcgg atacggatgc gcataaatga tgaggatcat agttattagc
    18661 ccgcgtgttt acgcctttgt tttcccacaa agtgtagtca tatattcacg cggcttatag
    18721 tcaataatat tgattcagaa aagagagaaa tctgaacaaa ttatcccatt ctctgcccgc
    18781 ggatcgtcat attgctggtc tgcgccagtg acatccctcg agtctccggg gcaaacgcta
    18841 cggaaatcaa caagccaaac agcgagatac ccgcccccat tagcatcgtg ttactgatac
    18901 cgtaattatt gataaagatc ggtagtgccc aggtcgaaac aatggtgcca atacgactta
    18961 aggacataat cacgcccacg gcagaggcgc ggatatctgt cgggaagagt tcattaggat
    19021 agagccactg caaattaccc ggcccgccag agaaaaaggc atacaccgca aaggccatca
    19081 ctaccagcca gatccccata tccgggatta gccccaaaac cgccagcgcc agcgtcatca
    19141 tggcaaagct gccaatcaac aatggacgcc gtccggcagt gtttaaccac agcatcggcg
    19201 gaatacagcc gagcataaag aacaggctaa tcaccacatt ccctagtgcc gcgtttttgc
    19261 caacccccaa tcccaacaaa ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg
    19321 ggatcacctg gcaggtccag atggcggcaa caaacagaac aaaaggaaaa tggcggcgat
    19381 taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc atcgaaagcc accggttcgc
    19441 caaacagttt tatcatcatc tcttcgcact cttttactcg ccctttgcgt aataaccagc
    19501 ggggagattc aggcagttcg aatcgaccaa tcaaaatcaa caaacagggg atcgctgcgc
    19561 tacccagcat ccagcgccag ccgccttcca catcataaag ccagtagccg accagatcgg
    19621 cgcaggtcgc gccgacatac cacatggcgg cgataaagct gatggaaaat gcccgctgac
    19681 gggtactgga gaactcggtg atcattgagg tggcgatggg ataatctgca ccgatgacaa
    19741 tgccgataag tacccgcatc accaacagtt cgacggggga tgaaacaaac atcgtcgcca
    19801 ccgatatcac gccgatggcg atgatatcaa tgaggaacat tttgcgccgt ccgactttat
    19861 cggaaatata gccaaacagc gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca
    19921 acaagccaat ccagtcagcg tccagtttta gcgccggagt cagttgctcc agcgccacgc
    19981 caatcattac cagtacataa ccatccagaa acggaccacc gcttccccac agcaaaatgc
    20041 ggcggtgaat agaggagaat ttgagatcgt caaagtttct ggacggttgc atgggttatt
    20101 cctgtttttt gcaatgagag tcagtgcggc gttacgtatc aaaccaacat cagccgtaac
    20161 ggaactccac accaaaggtg ccgcgcgggt attcccactg ttccagcgcc gaccccagcc
    20221 ccagaatgcg acaggtgccg cactccagac atccggcgta atcgaagcgc acactgccgt
    20281 catcctgctt cttgtacaga cctgcggggc acgctttcac cagcagctct agggcctgtt
    20341 tatcggcatc ggccttcaca acaatgtgtg gatgctcttc atcgacattg aatttattga
    20401 cgcccagttt gacgtccaca ttgacgggag aagtcataaa acggtcactc ctttcatgcc
    20461 atccttgatc agattgatga agcccacttt cttgccgtgg cggaggattt tcttgcgcat
    20521 cagttccggc gcactgccgt caatggtgaa caggtcacgc gccacgccca ccgccagttc
    20581 tgggtagccg ctaaacatgc gtgggttatc aaggaacgcc ggtagtttct ggtacatgcg
    20641 catatcgcgc agcgggccac tctccagatg ctgacgatat tccgcgagtt tttgcttact
    20701 gaaatcgtcg cttttcatcg ctgaaagcac ggtttttgct gcagcttctc cggcggcaat
    20761 cgccagatcc ataccgcgaa tggtaaaacc gaggttcata cacattccgg cggcatcacc
    20821 ggcaatcaat acgccgtcac cgaccagctc cggcagcatg ttgatgcctg cttccggcac
    20881 tacgtgagcg gaatattcca ccagcttgcc acccgcgatc agcggtgcaa cggccggatg
    20941 ttgtttgaaa tcttccagca tttgcggcac cgattttttt gcgtcatgca gatgatgcag
    21001 accacaaacc agccccagcg acagggtatt ttcattggta taaaggaagc cgccgcccat
    21061 caagccatcg gtgggcgatc ccgcaaacag gcaagccgta ccctgattac cctgcaactg
    21121 aaaacggtct tcgattaccg acttcggtaa ctcgatcagt tccttcacgc caaccgccac
    21181 atccgtcggt ttaacgcgtt ttgccatccc caacttttcg gcaaggatgg agttcacccc
    21241 atcagcaagg atcaccgttt tcgcttcaat cacatcgcca tcggcttcta caccgacgac
    21301 tttgccatcg cgctgtacga ggttatcgac acggatcccg gtaattaact gcgcgcccgc
    21361 ttcttcggcc tgctccatca accaggcatc aaatttactg cgcaaaacgg agtaagaacg
    21421 ctgggatggc gaggtttcgt caccgttgca gtagtccata gtcatcgctg acttttccgt
    21481 cataaacgcg agtttttcat gggtgatcag gcgttctacg ggggcggagt cggcgaaacc
    21541 aggaataatg tgttccagac tgtgggcata gagacgcccg ccggtgacgt tcttggcacc
    21601 tgcggaattg ccacgctcga taactaacac ttgcgcacct tcgcgggcga gcaccagtgc
    21661 ggcaaccgaa ccggcaagcc ctgcaccgac gatgatggcg tcaaagatat cttcggacat
    21721 aagagctccc tgtcagcggc agggccaaaa tgcagccctg ccagagtgga tcaacgcgct
    21781 aaagctgcgg tcagcgccgg aaggatcttc acggcgtcgc caacaatgcc gtaatccgcg
    21841 tactggaaga tcggcgcatt tttatcttta ttgatggcga aaatggtttg cgacgcgtta
    21901 gcgccaacca tgtgctggat ctgcccggag atccccaccg ccaagtacag ttcaggtttc
    21961 agcatcaggt tggagatacc gacatagcgt tcgtgctcca tccatttttc gttttccgcc
    22021 accggacgag aacaggccaa ctccgcacct atcgccttgc aaagctgttc tgccagcgca
    22081 atgttctctt tgctgccaat accgcgaccg acgctgacca ccagacgggc tttgtcgaga
    22141 tcgacgctgt tgctctggcg cgcctgggtt gccgtgcggg taatcgccac agccggagcc
    22201 tgccactcca cggtgtgcgt ttcgccagtg cgtgacgcgt ctggctgagc cgcatcgaac
    22261 gtgccgctgc tgatggtcag taccgcatac ggcgtggcaa tgcgttcttc gccaatcgcc
    22321 agaccaccgt aaaccatgtg tttcactgtc gctttaccgt cctgtacgct gacggtgctg
    22381 gcatcgttag acaccgccgc tttaaggcga taacccagtt ttgccgccag taatttgccg
    22441 cgacgggtgt ttggcagcag caccaggccg tctgcgccgt gctggcgaat agtgtcagcc
    22501 atgacaccgg cgtaatcttc gatcatccga tcgtccggtt tgccgtttaa tttccagaca
    22561 tgattagcgc cgagctggat tgcctgtgcg ccgtcggcat cattgaggac aaaggtgttg
    22621 atttgattag ctaaagcctg cgcaccgttc atcagttccg gcagacgaga aggggtatcg
    22681 ctgaataccc agacttgaga aaacgtgttc atagcatccc ctgtaattaa atgactttgc
    22741 gaagattttc agcaaatgcg gcgatctgtt cttcgccgtc gccttcaatc acgatgcgct
    22801 gacgttcgcg ctgtttcggc gcggcaacct gttgttctga ccaggctgcc tctgcgttaa
    22861 aaccaatatc cgccgccgac catacctgga cgggcttttt cgccgcgccg agaatggctt
    22921 tcatcgaagg aatttgtggg gaattgatat cagtggaaac agcaacaacc gcaggcagcg
    22981 gaatgcttaa ggtttcggtt tcatcttcca gttcgcgctc aacggtgagg gtatctgcag
    23041 tcagggagat aattttgctg acgccgttaa ctgccggaat attgaggatt tcgcccacca
    23101 gcagaccaac ctgctgggca taaaggtcgg aagaaccatc gccacagagg atcaaatcaa
    23161 agcctgcttt ctgggcggct gcagccagtg cgctcgccgt ttgttgcggc agtgcctgct
    23221 cgaactggtc atcaatcacc acaatcagtt catccgggcc gcgcgatagc acatctttac
    23281 gccctttggc gttggtcagg gctttaccgc ccacacttaa ggccgtcacc tgcgcctctg
    23341 ctgcctgttg ctttagctgg caagccgctt caatagcgtt cagatcgtat tggcttattt
    23401 tggcatcggc tttgctgaag tctaatgaac catcagcatt attgaccgca atatcctgtt
    23461 catcaggcac gcacttatag caagtaataa tcttcattgc atctccagaa atcatgaagg
    23521 aaacgtcgtt catatcggtg gtgttaatta cagatatcac gccctttaat ttggaaataa
    23581 aactttaatc accaatattg aaaatgtcac acgcagataa atccaacttt caatattgtt
    23641 aagttcctca ccaatattga aaacatggcg tagcaaaaag aaattttcaa tattgcttta
    23701 tggatcacca atattgaaag tcagatgcta tttttttact cacaacacaa cataacaaac
    23761 tgattaataa tcaaaattaa cgagaaaagc gccctctatt cacccgaaac aaaaatgtga
    23821 taacaatcac agaatgcagc ttattgaata cccattatga gttagccatt aacgcgtcca
    23881 cgaggttgat aataattata ttaaatgtta acaaaaataa aacaaacggg aacgcaaaat
    23941 aaatattcgt tttcacagtg gaattaactc atgaagaatg aaaagagaaa aacgggaata
    24001 gaaccgaagg ttttctttcc gccgttaata atcgtcggca tactttgttg gcttacagtc
    24061 agagatctgg atgcagcgaa tgtcgttatt aatgctgtat tcagttacgt caccaatgta
    24121 tggggatggg catttgaatg gtatatggtg gtgatgcttt tcggttggtt ctggctggtg
    24181 tttggcccgt atgccaaaaa gcgtttaggt aacgaaccac ctgaatttag taccgccagt
    24241 tggatcttta tgatgttcgc ctcctgtacg tctgctgccg tactgttctg gggatcgatt
    24301 gagatctact actacatctc caccccgccg tttggcttag aaccgaactc gacaggggcg
    24361 aaagagttgg ggctggctta cagcttgttc cactggggac ctctgccgtg ggccacttac
    24421 agcttccttt cagtcgcctt cgcttacttc ttctttgtcc gcaaaatgga agtgattcgc
    24481 cccagctcga cactggtgcc gctggtaggt gaaaaacacg ccaaagggtt gttcggcact
    24541 atcgtcgaca acttctatct cgtcgccttg atcttcgcga tgggtaccag tctgggcctt
    24601 gccacgccgc tggtgaccga gtgtatgcaa tggttgtttg gcattccgca taccctgcaa
    24661 ctggacgcta tcatcattac ctgctggatt atcctcaacg ccatttgcgt cgcttgcggt
    24721 ctgcaaaaag gggtacgtat cgccagtgac gtgcgtagtt acctgagctt cctgatgctg
    24781 ggttgggtgt tcattgtcag cggtgccagc ttcatcatga actacttcac cgattcggtg
    24841 gggatgttgc tgatgtatct gccgcgcatg ttgttctata ccgatcccat cgctaaaggc
    24901 ggcttcccgc agggctggac cgtgttctac tgggcatggt gggtgattta tgctatccag
    24961 atgagtatct tcctcgcccg catctcccgt ggtcgtactg tgcgtgaact gtgcttcggc
    25021 atggtgctgg ggctgacagc gtcaacctgg atcctgtgga ctgtactcgg tagtaacact
    25081 ctgctgttga tagataaaaa catcatcaac attccaaatc tgatcgaaca gtacggtgtg
    25141 gcgcgcgcca tcattgaaac ctgggccgct ctgccactca gcaccgccac catgtggggc
    25201 ttcttcatcc tctgctttat tgccaccgtt acgctggtta acgcctgctc ttataccctg
    25261 gcgatgtcca cttgccgcga agtacgcgat ggtgaagaac cacctctgct ggtgcgtatc
    25321 ggttggtcaa ttctggttgg cattatcggt attgttctgc tggcgctcgg cggcctgaaa
    25381 ccgattcaaa ccgccattat cgccggagga tgcccgctgt tcttcgtcaa cattatggtg
    25441 acgctctcct ttattaaaga cgcgaaacag aactggaaag attaattacc ccccaaaata
    25501 tcaagaggtt gaaagatgga ttttaattta aatgatgagc aggaactgtt tgtcgccggt
    25561 atccgcgaac tgatggccag cgaaaactgg gaggcctatt ttgccgagtg cgaccgtgac
    25621 agcgtctacc cggaacgttt tgtcaaagca ctggcggata tgggtatcga cagtctgctg
    25681 atccctgaag agcacggtgg tctggacgcg gggtttgtta ctctcgccgc cgtgtggatg
    25741 gagctgggac gtctgggggc accaacctat gtactgtacc agttgccggg cgggttcaac
    25801 accttcctgc gcgaaggcac acaagagcag atcgacaaga ttatggcttt ccgcggcacc
    25861 ggtaagcaga tgtggaactc agcgattacc gaaccgggcg cgggctccga cgtgggtagc
    25921 ctgaaaacga cttatacccg tagaaatggt aagatttatc ttaatggtag taagtgtttt
    25981 attaccagca gcgcctacac cccgtacatc gtggtgatgg cgcgcgacgg ggcttctccg
    26041 gacaaacctg tctacaccga atggtttgtt gatatgagca aaccgggcat caaagtgacc
    26101 aaacttgaaa agctcggtct gcgtatggat agctgctgtg aaatcacctt tgacgacgtg
    26161 gaactggacg agaaagacat gttcggtcgg gaaggtaacg gctttaaccg cgtcaaagaa
    26221 gagttcgacc atgaacgttt cctggtagcc ctcaccaact acggtacggc gatgtgcgcc
    26281 tttgaagatg cggcgcgcta cgccaaccag cgcgtgcagt ttggcgaggc tattggtcgt
    26341 ttccagttga ttcaggaaaa attcgcccac atggcgatca aattaaactc catgaaaaac
    26401 atgctgtatg aagcagcgtg gaaagcagac aacggcacca tcacctctgg cgatgcagcg
    26461 atgtgcaaat acttctgcgc caatgcggca tttgaagtgg tggatagcgc aatgcaggtg
    26521 ctgggcggtg tcgggattgc gggcaaccac cgcatcagcc gcttctggcg tgatctgcgt
    26581 gtagaccgcg tctccggggg atctgacgaa atgcagatcc tgacgctggg tcgtgcggtg
    26641 ctgaagcaat accgctaagt ccgtgcgctg cctgatgcga cgctgacgcg tcttatcagg
    26701 cctacgggag cacgaatgta ggccggataa agcgtttacg ccgcatccgg cagtcatgcg
    26761 ctacataaat ttttcaggag caaataatgg atcatctacc catgccgaaa ttcgggccgt
    26821 tggccggatt gcgcgttgtc ttctccggta tcgaaatcgc cggaccgttt gccgggcaaa
    26881 tgttcgcaga atggggcgcg gaagttatct ggatcgagaa cgtcgcctgg gccgacacca
    26941 ttcgcgttca accgaactac ccgcaactct cccgccgcaa tttgcacgcg ctgtcgttaa
    27001 atattttcaa agatgaaggc cgcgaagcgt ttctgaaatt aatggaaacc accgatatct
    27061 tcatcgaagc cagtaaaggt ccggcctttg cccgtcgtgg cattaccgat gaagtactgt
    27121 ggcagcacaa cccgaaactg gttatcgctc acctgtccgg ttttggtcag tacggcaccg
    27181 aggagtacac caatcttccg gcctataaca ccatcgccca ggccttcagt ggttacctga
    27241 ttcagaacgg tgatgttgac cagccaatgc ctgccttccc gtataccgcc gattactttt
    27301 ctggcctgac cgccaccacg gcggcgctgg cagcactgca taaagcgcgt gaaaccggta
    27361 aaggcgaaag tatcgacatc gccatgtatg aagtgatgct gcgtatgggc cagtacttca
    27421 tgatggatta cttcaacggc ggcgaaatgt gcccgcgcat gagcaaaggt aaagatccct
    27481 actacgccgg ttgcggtctg tataaatgtg ccgacggcta catcgtgatg gaactggtgg
    27541 gcattaccca aattgaagag tgctttaaag atattggcct cgcacatctg cttggcacgc
    27601 cagaaatccc ggaaggcact cagcttatcc accgtatcga atgcccttac ggcccactgg
    27661 ttgaagagaa actcgatgac tggctggcga cacataccat cgcggaagta aaagaacgct
    27721 ttgctgaact gaatatcgcc tgcgccaaag tgctgaccgt accggaactg gaaagcaatc
    27781 cacagtatgt ggctcgcgaa tcaatcactc agtggcaaac gatggatggt cgcacctgca
    27841 aagggccgaa catcatgccg aaattcaaaa ataaccccgg acaaatctgg cgcggaatgc
    27901 cctcacatgg catggacacg gctgccattt tgaaaaatat cggctacagc gaaaacgaca
    27961 ttcaggagtt ggtcagcaaa ggtctggcca aagttgagga ctaaacgcgt ggcgtcagtc
    28021 ggcttgcctg actagcgccc gcccaataac aaacgaacaa aaatggatag aggtgcaatg
    28081 gatatcattg gcggacaaca tctacgtcaa atgtgggacg atcttgcgga cgtttacggt
    28141 cataaaacgg cgctgatttg tgaatccagc ggcggagtcg ttaaccggta tagttatctt
    28201 gagttaaatc aggagattaa ccgcacggca aacctgtttt atacgctggg gattcgcaaa
    28261 ggcgacaagg ttgcactaca tctcgacaac tgcccggaat ttatcttttg ctggttcggg
    28321 ctggcaaaaa ttggcgcgat tatggtgccg attaacgccc gcctgttacg cgaggaaagc
    28381 gcgtggatcc tgcaaaatag ccaggcgtgc ctgctggtga ccagtgcgca attctatcct
    28441 atgtatcaac agattcagca ggaagatgcc actcaattgc ggcacatttg cctgacagat
    28501 gtggcacttc ccgctgatga tggcgtgagt tcgtttactc aactgaaaaa tcaacaacct
    28561 gccaccttgt gctatgcacc gccgctatcg actgacgata cggcggaaat tctcttcacc
    28621 tccggcacca cctcccgacc gaaaggtgtg gtgattaccc attacaacct gcgcttcgct
    28681 ggatattact ccgcctggca gtgtgcactg cgtgacgatg acgtctacct gacggtaatg
    28741 cctgcgtttc atatcgattg ccagtgtact gcggcgatgg cggcgttttc tgccggggcc
    28801 acctttgtgc tggtcgagaa atacagcgcc cgcgccttct ggggacaggt acagaagtac
    28861 cgcgccaccg ttaccgaatg tattccgatg atgatccgta ccttgatggt gcagccgcct
    28921 tcagcgaacg atcggcaaca ccgcctgcgg gaagtgatgt tttatctcaa cttgtcggag
    28981 caggaaaaag atgcgttttg tgaacgcttc ggcgttcgct tgctgacgtc ttatgggatg
    29041 acggaaacca ttgtgggcat tatcggcgat cgccctggcg ataaacgacg ctggccgtcg
    29101 attggtcgag cggggttttg ctacgaagcg gagatccgcg acgatcacaa ttgcccgctc
    29161 ccggcaggtg agatcggtga aatctgtatt aaaggcgtac cagggaaaac catcttcaaa
    29221 gagtattttc tcaacccgaa agccactgcg aaagtgctgg aagctgatgg ctggctgcat
    29281 accggcgata ccggataccg cgacgaagaa ggcttttttt atttcgtcga tcgccgctgc
    29341 aatatgatca aacgcggtgg cgagaatgtc tcctgcgtgg agctggaaaa tatcatcgcc
    29401 acccatccga aaattcagga catcgtggtt gtgggtatta aagattcgat tcgcgatgaa
    29461 gccatcaaag catttgtggt gctgaatgaa ggtgaaacat tgagcgaaga ggaatttttt
    29521 cgcttctgcg aacaaaatat ggcgaaattt aaagtgccct cttatctgga gatcagaaaa
    29581 gatctgccac gtaattgctc ggggaaaata attagaaaga atctgaaata aatgaaacgg
    29641 cagggaacta ccctgccagc taataatcac gctctaaaac aatatgcatt ttttgcagga
    29701 atgttgtcgt ccctgaaaaa gcaaaaatgg agaaaaggaa tgagtgaatc attacatctg
    29761 acccgcaatg gatcaattct ggaaattacc cttgatcgcc caaaagcgaa tgctattgat
    29821 gcaaaaacca gctttgaaat gggcgaagta tttctaaatt tccgtgacga tccgcaatta
    29881 cgtgtcgcca ttattaccgg tgccggagag aaattctttt ccgcgggctg ggatttaaaa
    29941 gcggcagcag aaggcgaagc accggatgct gactttggcc cgggtggttt tgcgggatta
    30001 accgaaattt tcaatctcga caaaccagtt atcgcagctg tgaacggcta cgcctttggc
    30061 ggcggtttcg aactggcgct ggcggcagat tttattgttt gtgccgataa cgccagcttc
    30121 gccctgccgg aagccaaact gggcgtcgtt cctgacagcg gcggtgtgct gcgtctgccg
    30181 aagatcctgc cgcctgccat cgtcaatgaa atggtgatga ccggcagacg aatgggcgca
    30241 gaagaggcgc tgcgttgggg gatagtcaac cgcgtggtta gccaggcgga actgatggat
    30301 aacgcccgcg aactggcgca gcagctggtt aacagcgctc cgctggcgat tgcggcgctg
    30361 aaagagatct accgcaccac cagcgaaatg ccggtagaag aagcgtatcg ctatattcgc
    30421 agcggcgtgt tgaaacacta tccgtcggtt ctgcattcgg aggatgccat tgaagggccg
    30481 ctggcgtttg ccgagaagcg cgatccggtg tggaaaggac gttaacaacc gtgagctatt
    30541 acgcctttga ggggttaatt ccggtggttc acccgacggc gtttgtccat cccagtgccg
    30601 tcttgattgg cgatgtgatt gtgggagccg gtgtctacat cggcccactc gcctcactgc
    30661 gtggtgacta cgggcggttg atcgtgcaag cgggagccaa tattcaggat ggctgcatta
    30721 tgcatggcta ctgcgacact gacaccatcg ttggggaaaa cggccatatc gggcacggag
    30781 cgatcctgca tggttgtgtg attggtcgcg atgcattggt cgggatgaac agcgtgatta
    30841 tggatggcgc ggtcattggc gaagagagca ttgttgccgc catgagcttt gtcaaagcgg
    30901 gctttagcgg cgagaaacgc cagttgttga tgggtacgcc agcccgcgct gtacgcagtg
    30961 ttagtgacga cgagttacac tggaaacggt tgaataccaa agagtatcag gatcttgtcg
    31021 ggcgctgtca tgcatcgtta catgaaacgc agccgttgag gcaaatggag gaaaatcgcc
    31081 cccgtttgca ggggacaacg gatgtgacgc ctaaacggtg atcttgccgg atgcggcgta
    31141 aacgccttat ccaactctgc tggagagcaa ccgtaggccg gataagatgc gccagcatcg
    31201 catccggcga tagctgtcgg atgcggcata aacgccttat ccaacccggc tggagagcaa
    31261 ccgtaggctg gataagatgc gccagcattg catccagcaa ccaggctgcg tggtatcaac
    31321 gaaatgacat taacgacgca tactctttga caacatcatc tgccacttct gctcgcggtt
    31381 aagttccgtg agcggaacgg caggaatacg cggcgtcttt cttacaccga ctaatttttc
    31441 ccgactgtta ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca ctttaaccag
    31501 tcgctgacac tggcatcccc gcccttccag cttattaggg atcatcttaa cttcgcagct
    31561 tatttctgtg acttccgaca gaatataagt cagggtatta accgccttgc tgtgttcaat
    31621 atcgaaatga atagagatct ctcttgctat cacccaccga ttttcagcca tcatccattc
    31681 ggcgattaac aagtagagtg gtttttcaac atatccttca cacatacgaa ctccagtttc
    31741 acaaattaaa agtctggcta tgactcgttt tgaagcaatt aaacaaggcc atattaaaat
    31801 tgtggatata tcgatagtat gcaacttcac cgtcgataag tgtgagctaa atcctgctta
    31861 tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa aacggtcctc
    31921 atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccatgaca
    31981 cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg
    32041 gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta
    32101 tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg
    32161 gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct
    32221 tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg
    32281 ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg
    32341 cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag
    32401 ttgctgccca gctgcgcttt ggcaaacgct tcagcgaagg tgcggcccac gcccatgact
    32461 tccccggtag agcgcatttc tggccctaac agcgggtcaa cacccgggaa tttattgaac
    32521 ggcagcacca cttctttcac cgagtagtac ggcgggataa cttctttggt tacgccctgc
    32581 tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg
    32641 gctttggaga cgaacggaac ggtacgcgcc gcacgcgggt taacttcaat caggtagact
    32701 tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc
    32761 agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt gtaggctggc
    32821 agagaacatg cggagtcacc ggagtgcacg cccgcctgct cgatgtgctc catgatgccg
    32881 ccaataagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac cgcgtcatca
    32941 aggaagtgat ccagcaacac tggcgcatcg ttagacacgc tgaccgccgt ctggaagtag
    33001 cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac
    33061 ggacgtacca ccagcgggta gccaatctct ttcgccttct ctaccgccat ttcaatagcg
    33121 gtaacggtgg cgttcgccgg ttgtttcagt ttcagacgct caaccgcatg ctggaagcgt
    33181 tcacggtctt ctgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagca
    33241 gcttccagcg cgcgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct
    33301 ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag
    33361 aggcgatcgg aagtgtcgta gtcggtggag acggtttccg ggttacagtt aaccataatg
    33421 gtttcgtaac cgtcttcgcg cagcgccagc gaggcgtgta cacagcagta gtcgaattcg
    33481 ataccctgac cgatacggtt cgggccgccg ccgagaacca tgattttttc acggtcggta
    33541 gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggcg
    33601 aactctgccg cacaggtatc cacgcgctta taaaccgggt gcaggtcata ctggtcgcgc
    33661 agcttgcgga tttccgcttc gcgcacgccc gccagttttg ccagacgcgc atcggcaaag
    33721 cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgct
    33781 actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta
    33841 gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag
    33901 atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca
    33961 tcgaggctca ctttcgggtc gaatccagtc gcaccgactt ccaggccgcg cagcgctttt
    34021 tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga
    34081 gtggtcagac ggtcgttagc accagcgaat ttttcgaagt tgaagcgagg aattttagta
    34141 accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc
    34201 atcagttcgt cgagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa
    34261 ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca
    34321 atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg
    34381 ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc
    34441 agcgtttggg ctggcgcgac agtgatggag tcaccggtat ggatgcccat cgcatcaaag
    34501 ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg
    34561 tactctttcc agccgatcag cgactcatca atcagcagct ctttggttgg ggagagatcc
    34621 agaccgcggg cgcaaatttc ttcaaactct tcgcggttat aagcgatacc gccgccgcta
    34681 ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaaccgcc
    34741 agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc
    34801 atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc ggtcgcacca
    34861 atcatagtca cgccgaactc ttccaacacg ccctgacgct ccagctccag cgcgcagttc
    34921 agcgccgtct gaccgcccat cgttggcagc accgcgtccg ggcgctcttt ttcaataatc
    34981 ttgcgtacta cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg
    35041 gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg
    35101 gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg
    35161 cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc
    35221 ctgattactt agcggtttta cggtactgct caattaactc gataaagtgg tcgaacaacg
    35281 gcgctgcgtc gtgtggccca gggctggctt ccgggtgacc ctggaagctg aacgccggtt
    35341 tatcagtgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca
    35401 ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca
    35461 ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt
    35521 tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata
    35581 ccggaatatc ggtttcgagg aatttctgga tggcggtaat ggcgtaatcg cacggggccg
    35641 ggtcgccagg accgttggag aggaagatac cgtctggatt cattttcagc acatcttccg
    35701 cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga
    35761 tgttgcgttt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt
    35821 tcgcttctgg catgccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg
    35881 cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt
    35941 ctaacgccag cgccgcatcc gggttatcgc ccgcgataat gcagccattc tgtgcgcctt
    36001 tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc actatgttat
    36061 ggcgtttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca
    36121 ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag
    36181 tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat
    36241 cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg
    36301 aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg
    36361 ctgacttaat caaaacaccc tccagagaat attcactcac tttatttgca tattaattca
    36421 caatgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca
    36481 ttctggagat atgggcgcta aaagtcaact taatggcggg attttttata ttattttgtg
    36541 gctcatgcat gttttacatg cattaagcga caaaaagatc atataacctg ttctggaaaa
    36601 cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc agggaaaatt ggaccaaatg
    36661 gtcaaaattt acattaaaac aacaaaaacc gaagacaaaa catgtaattt ttggcactta
    36721 tagacaattt aaaaacataa cactaaaaa
//
LOCUS       DAOFQD010000035        36680 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.035, whole genome shotgun sequence.
ACCESSION   DAOFQD010000035 DAOFQD010000000
VERSION     DAOFQD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.035"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(8..1867)
                     /gene="gss"
                     /locus_tag="QBF46_001820"
     CDS             complement(8..1867)
                     /gene="gss"
                     /locus_tag="QBF46_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDQ6674009.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2072..2938
                     /gene="yghU"
                     /locus_tag="QBF46_001821"
     CDS             2072..2938
                     /gene="yghU"
                     /locus_tag="QBF46_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDQ6674010.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3061..3309)
                     /gene="hybG"
                     /locus_tag="QBF46_001822"
     CDS             complement(3061..3309)
                     /gene="hybG"
                     /locus_tag="QBF46_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDQ6674011.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3322..3663)
                     /gene="hypA"
                     /locus_tag="QBF46_001823"
     CDS             complement(3322..3663)
                     /gene="hypA"
                     /locus_tag="QBF46_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDQ6674012.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3656..4144)
                     /gene="hybE"
                     /locus_tag="QBF46_001824"
     CDS             complement(3656..4144)
                     /gene="hybE"
                     /locus_tag="QBF46_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDQ6674013.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4137..4631)
                     /locus_tag="QBF46_001825"
     CDS             complement(4137..4631)
                     /locus_tag="QBF46_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDQ6674014.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4631..6334)
                     /gene="hybC"
                     /locus_tag="QBF46_001826"
     CDS             complement(4631..6334)
                     /gene="hybC"
                     /locus_tag="QBF46_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDQ6674015.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAVGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6331..7509)
                     /gene="hybB"
                     /locus_tag="QBF46_001827"
     CDS             complement(6331..7509)
                     /gene="hybB"
                     /locus_tag="QBF46_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDQ6674016.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7499..8485)
                     /gene="hybA"
                     /locus_tag="QBF46_001828"
     CDS             complement(7499..8485)
                     /gene="hybA"
                     /locus_tag="QBF46_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDQ6674017.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8488..9606)
                     /gene="hybO"
                     /locus_tag="QBF46_001829"
     CDS             complement(8488..9606)
                     /gene="hybO"
                     /locus_tag="QBF46_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDQ6674018.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9795..10082)
                     /locus_tag="QBF46_001830"
     CDS             complement(9795..10082)
                     /locus_tag="QBF46_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDQ6674019.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10201..11091)
                     /locus_tag="QBF46_001831"
     CDS             complement(10201..11091)
                     /locus_tag="QBF46_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDQ6674020.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKL
                     MNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTAD
                     VAKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYD
                     KSAADLAWQRTLKWFDKYLS"
     gene            11248..12288
                     /gene="gpr"
                     /locus_tag="QBF46_001832"
     CDS             11248..12288
                     /gene="gpr"
                     /locus_tag="QBF46_001832"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDQ6674021.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNDMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12328..12822)
                     /locus_tag="QBF46_001833"
     CDS             complement(12328..12822)
                     /locus_tag="QBF46_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDQ6674022.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13013..13897
                     /gene="yghA"
                     /locus_tag="QBF46_001834"
     CDS             13013..13897
                     /gene="yghA"
                     /locus_tag="QBF46_001834"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDQ6674023.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14169..14594)
                     /gene="exbD"
                     /locus_tag="QBF46_001835"
     CDS             complement(14169..14594)
                     /gene="exbD"
                     /locus_tag="QBF46_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDQ6674024.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14601..15335)
                     /gene="exbB"
                     /locus_tag="QBF46_001836"
     CDS             complement(14601..15335)
                     /gene="exbB"
                     /locus_tag="QBF46_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDQ6674025.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15587..16774
                     /gene="metC"
                     /locus_tag="QBF46_001837"
     CDS             15587..16774
                     /gene="metC"
                     /locus_tag="QBF46_001837"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDQ6674026.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16914..17573
                     /locus_tag="QBF46_001838"
     CDS             16914..17573
                     /locus_tag="QBF46_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6674027.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17613..18512)
                     /gene="yqhC"
                     /locus_tag="QBF46_001839"
     CDS             complement(17613..18512)
                     /gene="yqhC"
                     /locus_tag="QBF46_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDQ6674028.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18706..19869
                     /gene="yqhD"
                     /locus_tag="QBF46_001840"
     CDS             18706..19869
                     /gene="yqhD"
                     /locus_tag="QBF46_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDQ6674029.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19974..20801
                     /gene="dkgA"
                     /locus_tag="QBF46_001841"
     CDS             19974..20801
                     /gene="dkgA"
                     /locus_tag="QBF46_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDQ6674030.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21001..21927
                     /locus_tag="QBF46_001842"
     CDS             21001..21927
                     /locus_tag="QBF46_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDQ6674031.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21978..22235
                     /gene="yqhH"
                     /locus_tag="QBF46_001843"
     CDS             21978..22235
                     /gene="yqhH"
                     /locus_tag="QBF46_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDQ6674032.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22278..24497)
                     /locus_tag="QBF46_001844"
     CDS             complement(22278..24497)
                     /locus_tag="QBF46_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDQ6674033.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALLGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVMPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24608..26020)
                     /gene="ftsP"
                     /locus_tag="QBF46_001845"
     CDS             complement(24608..26020)
                     /gene="ftsP"
                     /locus_tag="QBF46_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDQ6674034.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMNDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26095..26832)
                     /gene="plsC"
                     /locus_tag="QBF46_001846"
                     /gene_synonym="parF"
     CDS             complement(26095..26832)
                     /gene="plsC"
                     /locus_tag="QBF46_001846"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDQ6674035.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27066..29324)
                     /gene="parC"
                     /locus_tag="QBF46_001847"
     CDS             complement(27066..29324)
                     /gene="parC"
                     /locus_tag="QBF46_001847"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDQ6674036.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29462..31069)
                     /locus_tag="QBF46_001848"
     CDS             complement(29462..31069)
                     /locus_tag="QBF46_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ6674037.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31202..31597)
                     /gene="mqsA"
                     /locus_tag="QBF46_001849"
     CDS             complement(31202..31597)
                     /gene="mqsA"
                     /locus_tag="QBF46_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HDQ6674038.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(31599..31895)
                     /gene="mqsR"
                     /locus_tag="QBF46_001850"
     CDS             complement(31599..31895)
                     /gene="mqsR"
                     /locus_tag="QBF46_001850"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HDQ6674039.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(32100..32582)
                     /locus_tag="QBF46_001851"
     CDS             complement(32100..32582)
                     /locus_tag="QBF46_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDQ6674040.1"
                     /translation="MINLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPTSGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32635..33027)
                     /gene="ygiW"
                     /locus_tag="QBF46_001852"
     CDS             complement(32635..33027)
                     /gene="ygiW"
                     /locus_tag="QBF46_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDQ6674041.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33179..33838
                     /gene="qseB"
                     /locus_tag="QBF46_001853"
     CDS             33179..33838
                     /gene="qseB"
                     /locus_tag="QBF46_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDQ6674042.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33835..35184
                     /gene="qseC"
                     /locus_tag="QBF46_001854"
     CDS             33835..35184
                     /gene="qseC"
                     /locus_tag="QBF46_001854"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDQ6674043.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(35230..35562)
                     /locus_tag="QBF46_001855"
     CDS             complement(35230..35562)
                     /locus_tag="QBF46_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HDQ6674044.1"
                     /translation="MLKKQLLSIFEALLYIMLTYWLIDAFFAFNKYDWMLDSGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            complement(35569..36278)
                     /locus_tag="QBF46_001856"
                     /pseudo
     CDS             complement(35569..36278)
                     /locus_tag="QBF46_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834027.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36281..>36680)
                     /locus_tag="QBF46_001857"
     CDS             complement(36281..>36680)
                     /locus_tag="QBF46_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDQ6674045.1"
                     /translation="GNKYQINHVDEIFVLQFDHSLSREYNVVHHPIKFYKPIDKSSPL
                     LNAALSENEELSAIFNFYRINSGGCIERFYTIELQGAHLSNILTCYPHSITHAGNQPE
                     EVIVLNYKNITWKHHIASTESYSCWEERVF"
BASE COUNT         9118 a         9685 c         9093 g         8784 t
ORIGIN      
        1 acatgtatta ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca
       61 gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc gcccacggtg aaggtacata
      121 cctgaatgta tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct
      181 gctcggcaaa tttaccgctg gttttgtcca gcacctcttc atgatggctg accaggtcga
      241 tattgctacc acagcgaccg gcaatcggtt tcactgcgta acctgttttc accaattcat
      301 cattaacagt gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga
      361 tcggcagaat cgctttgttg ccggggatca ccgtccacag cggctcaaag actagcactt
      421 ccgggcgcag caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg
      481 gcaccgcagc aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg
      541 cccaggtttt ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag
      601 catcccagcc cagttcatcc aatccacgca agatacgcgt ttcaaagccc gcctggtgca
      661 gcgcctgctc cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat
      721 ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc caattcgtta atcagccctt
      781 ccgccggatt gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc
      841 ccgcttcggt atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct
      901 catccatgca gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga
      961 gacgcaaacg tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt
     1021 tcagcacctt gtcggttgcg tgaagataca tcaggtgcag ctcgttggtg gctttaatta
     1081 gctcctgctc ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct
     1141 gaccgttggc ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt
     1201 caaactggcc tttgttttcc agacgcgctc cgctgatttt cagcagctcg cctgcaattt
     1261 ccggctgcgg taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg
     1321 tgtcatcaaa agtgtctttc agggtatagc agccgttttc gaccaccatc tccagctcgc
     1381 gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga
     1441 ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc
     1501 ctttatccca gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg
     1561 cctgcaatgg caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct
     1621 cccacgccat acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag
     1681 caaattcaac gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata
     1741 cggcgtcatc ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta
     1801 ccccacctgg ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt
     1861 tgctcatcac tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg
     1921 attacctgct cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag
     1981 cggctggcgc aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc
     2041 ttcaacacta ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa
     2101 agtctggacg tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc
     2161 tggtccgacg catgaaaaaa cgctgccggt gggcaaacat ccattacaac tttattcgct
     2221 gggaacgccg aacggtcaga aagtaacgat tatgcttgag gagctgctgg cgctgggcgt
     2281 tactggtgca gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag
     2341 cggctttgtc gaagtgaacc caaactcgaa gatcccggca ttgcgcgatc atacccataa
     2401 tccgccgatc cgcgtgtttg agtctggatc gattctgctt tatctggcgg agaaatttgg
     2461 ctacttcctg ccgcaggatc tggcaaaacg gactgaaacg ctgaactggc tgttctggtt
     2521 acagggggct gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt
     2581 aaaaattgag tacgccatca atcgctttac catggaagcc aagcgcctgc tcgacgtgct
     2641 ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat gagtacacca ttgcggatat
     2701 ggcgatttgg ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt
     2761 tctggatgcg ggaagttata aacacgtaca acgctgggcg aaagaagtgg gcgaacgtcc
     2821 ggcagtgaag cgtgggcgta ttgttaaccg caccaacggg ccgttgaacg agcagttgca
     2881 tgagcgccat gacgccagtg atttcgagac gaatacggaa gataagcgtc aggggtaagg
     2941 attggtgttc gtcgcagcaa gccatccagg ccggataagg cattcacgcc gcatccggca
     3001 atcgagcaca atgcctgatg cgacgctgcc gcgtcttatc aggcctacga aagctaatca
     3061 tcacgcgctg gtaatgtcgt aatccatttg gcgcagcgcg tctaatgtgg ctttggcttc
     3121 gtcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagtag
     3181 atcggcaggg ttaccttcac aaatcagggc gatattcaca tcgcgcttga taccacatac
     3241 ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc
     3301 aatacacatt gttaactccg gttattcaac ttcaatactt ttgacgatca gcgaatcgcc
     3361 ggtatcgaca cgcaaccgct cgccatgaca gatcggacac tgcgcatcgt gctgatgaat
     3421 ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga cgatatgtaa
     3481 atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac ggacggcgct
     3541 ctcctcaacg caggagagcg caccaatttc cagccacacg gcggtgacgc gcttaacatc
     3601 gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag acaactcatg
     3661 cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg
     3721 gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg cgttggccct
     3781 cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga tattgcgaaa
     3841 caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc agaccaattt
     3901 tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata actgcactca
     3961 gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta aaatcagaaa
     4021 catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc cgggcaattt
     4081 cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct atctcttcag
     4141 tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc agccagaacc
     4201 tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat gtgtggctcc
     4261 agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc ggtgaagcgc
     4321 agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt ggtaaacaac
     4381 gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt tttcgacaca
     4441 atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag ctccattccc
     4501 gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg ctctaacgct
     4561 tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac ccctaagact
     4621 aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca tccactacgt
     4681 gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcacaact tccagcggtt
     4741 tattcggatc ggcaaccggt gtacccacca gcgactgctc gtaaggaccg acgtcatcat
     4801 tgaagttacg cgggccagag ttccaggttg atggaacaac tgcctggtag ttgctgatga
     4861 taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg aggaagccaa
     4921 cacctttgaa ctcacccgtt gccggaatgt tcggcttcac aaaggtggtg tgatcgcctt
     4981 tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc aattcacagc
     5041 agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc acttccagcg
     5101 tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc tgggtagatt
     5161 cgcgacctac cgccaatttc accagcatat tagccagcgg acccacttct accgttttgc
     5221 cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa ccatcataag
     5281 ccggaatggt ggtaccttcc cacggtgcct gcggcgcttc gtctttatac caggagtgct
     5341 tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg atcggacgat
     5401 acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg ccgtttttac
     5461 tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg cgcgtcagcc
     5521 attccgggta gaacgcggca ataactgcgg tatcaacctt ataaacctgt tcaacaaagt
     5581 cgctcagctt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc agcacgccca
     5641 aaccgtcgag gttgatcggg ttcgcgacac cacctaccgc caggttctga atgtgcggcg
     5701 ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc agcgcctgca
     5761 ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgcc gggtgacccc
     5821 agtagccatt ggcgaaaata cccaactgac cgctggcaac cagatcttta atcttgttct
     5881 gaactttggt gaactcttcc ggactgttca gatgccaggt cgaaacgcct ttcagcattt
     5941 cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag tccagcgccg
     6001 agagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga atgatgttac
     6061 ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc gcacgaacgg
     6121 aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc atccatgcat
     6181 cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta ccggaagccc
     6241 atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac
     6301 gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat gacgattatg
     6361 atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga tgtaagcgca
     6421 aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg ggaagtaggc
     6481 gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg ttgcacaacc
     6541 taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca gcttcgccac
     6601 acgcagaacg accagcggga agagcatcag caggacttca atccagaaca tcacggagta
     6661 gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc caaagcgcag
     6721 cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga acaggctctt
     6781 ttcatccgga ccgttgccac gcagacccgc ctgcaccagt gaaccttcaa agatgacaat
     6841 cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt catagctctg
     6901 ccacagcgga tgtaccttgt agcccgccga gatcatcagc gaccccattg aagactggtg
     6961 catggtcggc agcagcgcac cgagcgcgat gatgaagaac atcaccttgt tcagacgctg
     7021 tagcgacacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca gagccatcac
     7081 gccgatgtag atggtcatac agaccgccgt ctcgaacagt accgagttca cgttgaagtg
     7141 acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcaatag tgatcgacaa
     7201 gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca gcggatggta
     7261 ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac aggcaaagcc
     7321 ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc cgttcaggtc
     7381 agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac agatgacgat
     7441 taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg gttttggatc
     7501 atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca
     7561 cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg gtgtgttgaa
     7621 tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg agattttcat
     7681 aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg ccgtacagat
     7741 gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg
     7801 gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc gccatcagct
     7861 cttcacgcgt accgaaaatc accgcgcccg ccgggcacac ttctacgcag ccaggcagac
     7921 cgcctttatc gagacgttcc acacctttct ggttgcacag ctcgcactta tgcagcgcac
     7981 caaacgggtt gttgtagtcg tacttcggca cgttgtacgg acaggcgacc atgcagtaac
     8041 ggcaaccggt gcacacgtct ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca
     8101 gtgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata cactgtttct
     8161 taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg ctggtccaca
     8221 cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc
     8281 cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa gcctggcagc
     8341 ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc gggcggtttt
     8401 cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg caggaggctg
     8461 ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt tatctttctt
     8521 ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca gcccaaccac
     8581 accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag cgttaacatc
     8641 cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga tgcctttatg
     8701 gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta ccggccacac
     8761 gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg gccctttaca
     8821 gccgaggtgg tacaggcacc agccttcgcg gtggccttca tcaccgaact ctttggcaaa
     8881 acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac ggccataggc
     8941 gaaggtcgga cggtttttgt catccagttt cggcggtttg ccgtaagtga tgatgtgcgc
     9001 aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga taacggtttt
     9061 gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag ctgcggcaac
     9121 accgccccac gcagagcagg aaccgatagc gataatggct gctgcgcctt ccgccgcttt
     9181 gcggatgtga tccacaatag gctcaccggc aaccatgcaa taaataccgt tatcttttaa
     9241 tgggatggaa ccatccacca ctaacacata ctgccctttg tacttctcga gagcgttatg
     9301 tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat actccagaga
     9361 gatagtctcc agtacgaggt tttctaccgt tggatgcgtt gcacgaagca gagattccgt
     9421 acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg ggttagtaac
     9481 cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg ctaatgctgc
     9541 acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg tgttatctcc
     9601 agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca atcgcaaaat
     9661 caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga tatcatgata
     9721 ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta aagcaagata
     9781 atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg caacgtagaa
     9841 caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc atagcggcgc
     9901 gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc gtacatccgg
     9961 tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt cacattaacc
    10021 gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa atggttattc
    10081 atcgttcacc tcattcgcta tatattgtca tatatagcga tattttaggc aaagaacgac
    10141 ttttgcgcac gctatttttt catgcttgtt taagacgtaa tcccgtaaga gataaaccta
    10201 tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat cggcggcaga
    10261 tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg gatagatata
    10321 cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc agccctcgtt
    10381 gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct tcgccacatc
    10441 ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg gatacgcgac
    10501 agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca ctttgcctgt
    10561 cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca tcagtttggt
    10621 tggatcaacc tgctgttgca gctcacgacc tttatcatca ttacccggat aacctccaac
    10681 ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc gccgtgccac
    10741 atcttcgata tacggattca gtccacgatt ctcatgcacc accaccacca cggctggcgt
    10801 tttgccgctc atttttgcgg gtttcaccag ataaccccgt acctcgccgt gaccatttgg
    10861 tgaaggatac gtgatgtact cagcaacaat ctccgggtcg gtaaactcta cctgagtcgc
    10921 cagcgcataa tttggcttga gcaaatcaaa caacgctaat gccgtcatcc cgcccaccgc
    10981 atacttcgcc gcaaggttga ggaactcacg tttcgagatt ttcccgtgag cgtaatagtc
    11041 gtagtaatcc aacaactctt gtgggaaatc tttggcggtt aaacgcggca tcgttgcact
    11101 cctcaactgg tgttttttta agcaaagcat aagcacgtgt ttttgcccaa tttttcgtca
    11161 ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc taaactagaa acattgtttc
    11221 gaaattgaac ggtggaaagg agaggtcatg gtctggttag cgaatcccga acgttacggg
    11281 cagatgcagt accgctattg cggaaaaagt ggtttacgcc tgcccgcgtt atcgctcggt
    11341 ttatggcaca atttcggtca cgttaacgcg ctggaatcac agcgtgcaat cctgcgtaaa
    11401 gcgtttgatt taggcattac gcactttgat ttagccaaca attacgggcc gcctccagga
    11461 agcgcagaag agaactttgg tcgcctgctg cgggaggatt ttgccgctta tcgcgatgaa
    11521 ctgattatct ctaccaaggc tggctacgat atgtggcccg gcccttacgg ctctggcggt
    11581 tcacgtaaat acctgctcgc cagcctcgac caaagcctga agcgtatggg gcttgagtat
    11641 gtcgatatct tttactctca tcgcgtcgat gaaaatacgc cgatggaaga aaccgcctct
    11701 gcgctggctc atgcggtaca aagcggtaag gcgctgtatg tcgggatctc ctcttactcg
    11761 ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt ggaaaattcc gctgttaatt
    11821 catcaacctt cgtacaattt actaaaccgc tgggtggata aaagcggcct gctggatacc
    11881 ctgcaaaata acggcgtggg ctgcattgcc tttactcctc tggctcaggg attgctgacc
    11941 ggaaaatatc tcaacggcat tccgcaagat tcacggatgc atcgtgaagg gaataaagtt
    12001 cgtggcctga cgccgaaaat gctcaccgaa gccaacctca acagcctgcg cttattgaat
    12061 gacatggcac agcagcgtgg acaatcaatg gcgcaaatgg cgttaagctg gttgctgaaa
    12121 gatgatcgcg tgacgtcggt attgattggt gccagccgcg cggagcaact tgaggagaac
    12181 gtgcaggcgc tgaataatct gacatttagc accgaggagc tggcgcagat tgatcagcat
    12241 atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg ataaatgacc tgttaattac
    12301 gggcgcgaga accgcgcccg aatcagatca gtgattatga cgagtcagtc ggtcaagata
    12361 gcccatcaca aatgcagaga gcacaaacgt cagatggata atgacgtacc acatcagttt
    12421 gttatcaggg acatttttcg catccataaa gacgcgcagt aagtgaatgg aagaaattgc
    12481 cacaatcgac gctgctactt tgtttttcag cgacgttgca tccattttcc ccagccagtt
    12541 cagcttctct ttgttctcgg agatatccag ttgtgagacg aaattctcat aaccggaaaa
    12601 catcaccatc accagcaaac cgccaaccag cgtcatatcc accagcgaca gcaacacgag
    12661 gatcaaatct gattccgcca tcgagaagat attcggcagt acgtgaataa tctcctggaa
    12721 gaacttcagc gccagggcaa ctaacgccag cgaaaggcca aagtacacgg gggcaagcag
    12781 ccagcgagaa gcatacattg cattttcaag aaaacgttcc atacattcct gtcgtgtagt
    12841 taaaacgggc aacagtatat cgcaattgag taaacagcac gcaacagcaa atcaacatat
    12901 acgttaaacc tttgtcgcat aattctgagg tttagggatc ttcctggttc ccttatcaac
    12961 gcatcgagaa cgtctaccct taaaggacgt tgaacctgaa gggagaaaaa cgatgtctca
    13021 tttaaaagac ccgaccacgc agtattacac tggtgaatat cccaaacaga aacaaccgac
    13081 gccaggcatc caggcgaaga tgacaccggt accggattgc ggcgagaaaa cctatgttgg
    13141 tagcggtcgc ctgaaagatc gtaaagcact ggtgacaggg ggcgattccg gaataggtcg
    13201 cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg gcgatcagtt atcttcccgt
    13261 ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa gaatgcggac gcaaagccgt
    13321 tctgctgcca ggcgatttaa gcgatgagaa atttgcccgt tcgctggttc acgaagcgca
    13381 caaggcgtta ggcgggctgg atattatggc gctggtcgcc gggaaacagg ttgccattcc
    13441 ggatattgca gacctcacca gcgaacagtt tcaaaagacc tttgccatta acgttttcgc
    13501 gctgttctgg ctaacccagg aagcgatccc cctgctaccg aaaggtgcaa gtatcatcac
    13561 cacttcgtca atccaggcat accagccaag tccgcattta ctggactatg cggctacgaa
    13621 ggcggcgatt ctgaactaca gccgtggcct ggcaaaacag gtcgcggaga aaggtattcg
    13681 ggtgaatatt gtcgcgccag gcccgatctg gacagcactg caaatttccg gcggacaaac
    13741 gcaggataag atcccgcagt ttggtcagca aacgccgatg aaacgtgcgg ggcaaccggc
    13801 ggaactggcc cctgtatatg tttatctggc aagtcaggag tcgagctacg tcaccgcaga
    13861 agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa aatgcccggt tgtgaaaagc
    13921 aaccgggcat cattgtgaat tcactcttac ctgatgtggc gaaaacgcct tatccgccta
    13981 caaacgcatg aagattcaac atgttgcaat agattttgta ggcctgataa gcgtagcgca
    14041 tcaggcattg agcacctaat gccggatgcg tcgtgaacgc cttatccggc ctacaaacgc
    14101 acgaagattc aacatgttgc aatagatttt gtaggcctga tgagcgtagc gcatcaggca
    14161 attctacttt acttcgcttt ggcggtttct tcgccgacca gacctatctt caggtaaccc
    14221 gcctgatgca gcgtatccat taccttcatc aacgtctcgt aatcgacggt tttgtccgct
    14281 cggaagaaga tggtggtgtc tttcttgcct tcggttaacg cattcaacgc cgtaatcatt
    14341 gtttcatcgg tgaccggatc gttaccgata aacatcgagt tgtctgcctt caccgacaga
    14401 taaaccggtt tttccgggcg cggctgcggc gtgctggtag aagcaggcaa gttcaccttc
    14461 acatctaccg tcgctaacgg tgccgccacc ataaagataa tcagcagaac caacatcacg
    14521 tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt tatcgtcgag gttttcgtta
    14581 agatgcattg ccatcggata ttatcctgcg cgtaattttt gtgcgacacg aaccggatgc
    14641 gcagcggcgc tggcttccag atccaggtca cggctttgca gcaacaatac ctgcgctgca
    14701 acatcaccca gcatcgcttt aaagccgcca atctggcgtg caaatacgtt atagataacg
    14761 accgcaggaa tcgctgccac gaggccgatt gccgttgcta acagagcttc tgcgataccc
    14821 ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga taccaataaa gctgttcatg
    14881 atgccccaga ccgtaccaaa cagaccaacg aacggagaaa tcgcgccgat ggttgccaga
    14941 tagccgttac cgcggcccat ttgacgaccc actgcggcga cccgacgctc cagacggaag
    15001 ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt ctgacagttc cagctcattc
    15061 tgcgcttcat tgagcaaatg caggcttaag cttttgctac caaaatcagc ggcgatatcg
    15121 ttggcctggt ttaaggaacg cgcttcagcc agcagttgct gctcgcgctt aaggcgacgc
    15181 ttctgattga agaactctac gctcttactg aagaagattg cccaggtgac tacggaggcc
    15241 aaaataagcc caatcatcac gcacttaacg acaatatcgg cgtgctgata cataccccag
    15301 acggaaaggt ccgtctgcat taaattatta cccactgtgt atctccagga cgcaagtcac
    15361 aaaatctgcg cataataata tcaaaacgac gtcgaattga tagtcgttct cattactatt
    15421 tgcatactgc cgtacctttg ctttcttttc cttgcgttta cgcagtaaaa aagtcaccag
    15481 cacgccattt gcgaaaattt tctgctttat gccaattctt caggatgcgc ccgcgaatat
    15541 tcatgctagt ttagacatcc agacgtataa aaacaggaat cccgagatgg cggacaaaaa
    15601 gcttgatact caactggtga atgcaggacg cagcaaaaaa tacactctcg gcgcggtaaa
    15661 tagcgtgatt cagcgcgctt cttcgctggt ctttgacagt gtggaagcca aaaagcacgc
    15721 gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg cgcggaacgt taacccattt
    15781 ctccttacaa caagcgatgt gtgaactgga aggtggcgca ggctgcgtgc tatttccctg
    15841 cggggcggca gcggttgcta attcgattct tgcttttgtc gaacagggcg atcatgtgtt
    15901 gatgaccaac accgcctatg aaccgagtca ggatttctgt agcaaaatcc tcagcaaact
    15961 gggcgtaacg acatcgtggt ttgatccgct gattggtgcc gatatcgtta agcatctgca
    16021 gccaaacact aaaatcgtgt ttctggaatc gccaggctcc atcaccatgg aagtccacga
    16081 cgttccggcg attgttgccg ccgtgcgcag tgtggtcccg gatgccatca ttatgatcga
    16141 caacacctgg gcagccggtg tgctgtttaa ggcgctggat tttggcatcg atgtttctat
    16201 tcaggccgcc accaaatatc tggttgggca ttcagatgcg atgattggca ctgccgtgtg
    16261 caatgcccgt tgctgggagc agctacggga aaacgcctat ctgatgggcc agatggtcga
    16321 tgccgatacc gcctatataa ccagccgtgg cctgcgcaca ttaggtgtgc gtttgcgtca
    16381 acatcatgaa agcagtctga aagtggctga atggctggca gaacatccgc aagttgcgcg
    16441 agttaaccac cctgctctgc ctggcagtaa aggccacgaa ttttggaaac gagactttac
    16501 aggtagcagc gggctatttt cctttgtgct taagaaaaaa ctcagtaatg aagagctggc
    16561 gaactatctg gataacttca gtttattcag catggcctac tcgtggggcg ggtatgaatc
    16621 gttgatcctg gcgaatcaac cagaacatat cgccgccatt cgcccacaag gcgagatcga
    16681 ttttagcggg accttgattc gcctgcatat tggtctggaa gatgtcgacg atctgattgc
    16741 cgatctggac gccggttttg cgcgaattgt ataacattgc cacttttgga caattttgca
    16801 gacatttaat tgtgaaaagt cttaaattgt tgcgtccggg atcaaggcgt cccggacgaa
    16861 tcaggagtac aataggcaga taaaggctta aacgctgttc cacaggaaag tccatggctg
    16921 ttattcaaga tatcatcgct gcgctctggc aacacgactt tgccgcgctg gcggatcctc
    16981 atattgttag cgttgtttac tttgtcatgt ttgccacgct gtttttagaa aacggcctgc
    17041 tgcccgcctc atttttgcca ggcgacagct tgttgatact ggcaggcgca ttgattgccc
    17101 agggggttat ggattttctg cctacgattg cgattctgac cgccgcagca agtctgggct
    17161 gctggctaag ttatattcag gggcgctggt tagggaatac caaaacggtg aaaggctggc
    17221 tggcacagct tcctgctaaa tatcaccagc gcgccacctg catgtttgac cgccacggtc
    17281 tgctggcgct gctggctgga cgttttcttg catttgtccg tacgctgctg ccaaccatgg
    17341 cgggaatttc cggtctgcca aaccgccgct tccagttttt caactggtta agtggattgc
    17401 tatgggtcag cgtggtaacc agttttggct atgccttaag tatgattccg ttcgttaaac
    17461 gccatgaaga tcaggtaatg acgttcctga tgatcctgcc aattgccttg ttaaccgctg
    17521 gcttgttagg cacgctgttt gtggtgatta aaaaaaaata ctgtaacgcc tgacgatttt
    17581 ccccgttccc ggctgctgta ccgggaacgt atttaattcc cctgcatcgc ccgcattctt
    17641 gccgcatctt cccccggcgt cacaccgaag taacgtttaa actcacggct aaattgcgat
    17701 gcgctttcat agccgacgcg catcgctgct gcgctggcct tcatgccgtc atggatgatc
    17761 atcatccgcg ccttatgcag acggtaattc ttcaaatact gcaacggcga ggtgctggtg
    17821 acagacttaa aattatggtg gaacgccgat acgctcatgt tggcttctgc cgccagttgc
    17881 tcgacgctca ggttttcggt gtatttattc tcaatccgtt tcagcacgcg gctaatcaga
    17941 ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc cgccgcaagg tccggtcagc
    18001 acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa tacgcgcatc cagtggtcgc
    18061 tccatcacgt cgagtaaccg ctccgccgcg cataaaatct cttctgataa cgtggcggag
    18121 ttaatcccgc tggctgccat cgacggctgg aaatgctcat cttcgccaat gtccatcaac
    18181 agttcctgta actgcaaaat atcgacattg agacgcaacc ctgccagcgg cacctctgac
    18241 gtggcatagg tttcgcactc aaacggcaac ggcaccgtca gcagcaggta ttcattggca
    18301 tcataacgaa acacgcgttc attgatataa ccgattttat gcccggaaaa gagaattatg
    18361 atgccaggct cgtacatcac cggtgtacgt gcgaaaggtg tctcgccata caacaaacgc
    18421 acatcgggca acagttctga caaactattt tctttatttt tcagtttatt aactttatcc
    18481 gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta atttcttagg aataatgcgg
    18541 caatttgatt gtgcgcaatt ttgtagcatt tctccagcac tctggagaaa taggcaagac
    18601 attggcagaa atgagcattg agagccaggg cgctggcgat cacaatgaaa aacatcaggc
    18661 agatcgttct ctgccctcat attggcccag caaagggagc aagtaatgaa caactttaat
    18721 ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa
    18781 attcctcacg atgctcgcgt attgattacc tacggcggcg gcagcgtgaa aaaaaccggc
    18841 gttctcgatc aagttctgga tgccctgaaa ggcatggacg tgctggaatt tggcggtatt
    18901 gagccaaacc cggcttatga aacgctgatg aacgccgtga aactggttcg cgaacagaaa
    18961 gtgactttcc tgctggcggt tggcggcggt tctgtactgg acggcaccaa atttatcgcc
    19021 gcagcggcta actatccgga aaatatcgat ccgtggcaca ttctgcaaac gggcggtaaa
    19081 gagattaaaa gcgccatccc gatgggctgt gtgctgacgc tgccagcaac cggttcagaa
    19141 tccaacgcag gcgcggtgat ctcccgtaaa accacaggcg acaagcaggc gttccattct
    19201 gcccatgttc agccggtatt tgccgtgctc gatccggttt atacctacac cctgccgccg
    19261 cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca ccgtggaaca gtatgttacc
    19321 aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag gcattttgct gacgctaatc
    19381 gaagatggtc cgaaagccct gaaagagcca gaaaactacg atgtgcgcgc caacgtcatg
    19441 tgggcggcga ctcaggcgct gaacggtttg attggcgctg gcgtaccgca ggactgggca
    19501 acgcatatgc tgggccacga actgactgcg atgcacggtc tggatcacgc gcaaacactg
    19561 gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata ccaagcgcgc taagctgctg
    19621 caatatgctg aacgcgtctg gaacatcact gaaggttccg atgatgagcg tattgacgcc
    19681 gcgattgccg caacccgcaa tttctttgag caattaggcg tgccgaccca cctctccgac
    19741 tacggtctgg acggcagctc catcccggct ttgctgaaaa aactggaaga gcacggcatg
    19801 acccaactgg gcgaaaatca tgacattacg ttggatgtca gccgccgtat atacgaagcc
    19861 gcccgctaag ctttttacgc ctcaaacttt cgttttcggg catttcgtcc agacttaagt
    19921 tcacaacacc tcaccggagc ctgctccggt gagttcatat aaaggaggaa cgtatggcta
    19981 atccaaccgt tattaagcta caggatggca atgtcatgcc ccagctggga ctgggcgtct
    20041 ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa agcgttagaa gtgggttatc
    20101 gctcgattga taccgccgcg gcctacaaga acgaagaagg tgtcggcaaa gccctgaaaa
    20161 atgcctcagt caacagagaa gaactgttca tcaccactaa gctgtggaac gacgaccaca
    20221 agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact ccagcttgat tatatcgacc
    20281 tctacttaat gcactggccc gttcccgcta tcgaccatta tgtcgaagca tggaaaggca
    20341 tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg cgtgtgcaac ttccagatcc
    20401 atcacctgca acgcctgatt gatgaaactg gcgtgacgcc tgtgataaac cagatcgaac
    20461 ttcatccgct gatgcaacaa cgccagctac acgcctggaa cgcgacacac aaaatccaga
    20521 ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt tttcgatcag aaagtcattc
    20581 gcgatctggc agataaatac ggcaaaaccc cggcgcagat tgttatccgc tggcatctgg
    20641 atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc acgtattgcc gaaaactttg
    20701 atgtctggga tttccgtctc gacaaagacg aactcggcga aattgcaaaa ctcgatcagg
    20761 gcaagcgtct cggtcccgat cctgaccagt tcggcggcta acatgcaaat tctcccggtg
    20821 gcggtaatgt tccgctaccg gacttttcag aaatcattta ttcccctcgc gtcccgcccg
    20881 ttgttactct tccttgttca ggaatgccaa atataaggac atcatcatgc agagccggaa
    20941 gctcttaaaa gaacaactca tctatatccg ggataaacgc aacggagagg tgaaaaacag
    21001 atgaaaataa tacttctgtt tttagcagcc ctggcaagtt ttaccgtaca cgcacagccc
    21061 ccctcacaga ccgtagaaca aacagtccgg catatttatc agaactataa atcagatgcc
    21121 actgcccctt attttggtga aactggagag cgggcgataa cttctgcgcg tattcaacag
    21181 gcgcttaccc tgaacgacaa tcttacgctg ccgggcaata ttggctggct ggattatgat
    21241 ccggtttgtg attgtcagga ttttggcgat ctggtgctag aaagcgttgc gataacccaa
    21301 actgacgccg atcatgccga tgccgttgtg cgctttcgta tctttaaaga tgataaagaa
    21361 aagaccacgc agacactgaa aatggtggcg gaaaatggtc gttgggtcat tgacgatatt
    21421 gtcagcaatc atggcagcgt cttacaagca gttaatagcg agaatgaaaa aacgctggcc
    21481 gctttagctt cgttgcaaaa agaacagccg gaagcctttg ttgccgaact ctttgaacat
    21541 attgctgatt atagctggcc gtggacgtgg gtggtttccg actcttaccg ccaggcggtt
    21601 aatgccttct ataaaaccac cttcaagacg gccaataatc ccgatgaaga tatgcaaata
    21661 gaacggcaat ttatttacga caatccgatc tgttttggcg aagagtcgct attttcacgc
    21721 gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg cccgcattca tgttcgtttt
    21781 acgctgacca atggcaacaa cgaagagcaa gaactggttt tacagcggcg cgaaggcaag
    21841 tgggaaatcg ctgattttat ccgcccgaac agcggcagcc tacttaagca gattgaggca
    21901 aaaactgccg ccagattaaa gcaatgagct gaattaaata acaattagcc ggaacaataa
    21961 ataaaaggga acactatatg aaaacgattt tcaccgtggg agctgttgtt ctggcaacct
    22021 gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca gctggcgagc aatgtgcaaa
    22081 cattaaatgc caaaatcgcc cggcttgagc aggatatgaa agcactacgc ccacaaatct
    22141 atgctgccaa atccgaagct aacagagcca atacgcgtct tgatgctcag gactattttg
    22201 attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa aaatccccgg tagcgtgtca
    22261 gttgccgggg atatttttta acgtccaacc gccgctttag gccgtttctt cgcaccagca
    22321 ttcaccggac gagattgcgt agacgacgct ttttttgccg tagcaggcgt ctgacgctgg
    22381 gtcgccatcg gcgtatgttt cgtcaacgcc ggacgggtat tgcggttctg gcgacgagct
    22441 tcacgcatct cttcaatggt tggcgcaggc actaagcaat cgcgacggct accaatcaga
    22501 tgctttttac ccatcgcttc cagcgcctgg cggattaacg gccagtttgc cggatcgtgg
    22561 taacgcaaca acgctttatg caaacgacgc tgcttgtcgc ccttcggtac gaagacgtct
    22621 tcactcttat aaccaatctt cgccagcggg tttttcccgg tgtaatacat ggtggttgag
    22681 ttcgccagcg gcgatgggta gaagttctgt acctggtcga gacggaaacg atgctttttc
    22741 agccacagcg ccagattcac catatcttca tcacgcgtac cggggtgcgc ggagatgaaa
    22801 taggggatca gatactgctc tttacctgcc tgttttgagt aagtatcgaa cagctcttta
    22861 aagcggtcat agctgcccat gcccggcttc atcatcttcg ataacggccc ttcttcggta
    22921 tgttccgggg caatcttcag ataaccgccg acgtgatggg tcgccagctc tttgatatag
    22981 cgtggatctt ccacggcgat gtcataacgt acgcctgagg caatcaggat ctttttaatg
    23041 cctttcagat cgcgagcgcg gcgatagagg ttgatcgtcg gttcgtggtt agtgtccata
    23101 tgcggacaaa tatccgggta aacgcaggac aaacggcgac aagtttgctc tgcgcgtggc
    23161 gatttgcagc gcaacatata catgttggca gttggcccac cgagatcgga aatcacgccc
    23221 gtaaaacctg gaacggtgtc gcggatcgct tcaatctcat taatgatcga atcttccgaa
    23281 cggctctgaa taatgcgccc ttcgtgctcg gtgatagaac agaaagagca gccgccaaag
    23341 cagccgcgca taatgttgac cgagaaacgg atcatttcgt aagccggaat acgggcattg
    23401 ccataggccg gatgcggcac gcgcttgtac ggcagcgcaa agacgctgtc catctcttcg
    23461 gtagaaagcg gaatagcagg cgggttgatc cacacatagc ggtcgccatg tttttgcatc
    23521 aatgcgcggg cgcagcctgg gttagtttcg tggtgcagaa tacgcgaagc atgggcgtac
    23581 agcactttat cgcccttcac tttctcgaaa gaaggcagca acacgtaggt tttttcccac
    23641 ggtttcgggc gcggcggctg cacggttacg gctttggctt cctgcttttt cggcatcacc
    23701 ggtttgttat ccgcacacgg caaatcttcg ccatacggat gcggtatcgg gtcgattttc
    23761 ccaggagtat caagacgagt agaatccacg ccgctccagc ctagcaacgc ctcttttacg
    23821 ataatcgcag tattacgcac atcgcggatt tcgctaattg gctcgcccat cgccagacga
    23881 tgcgccactt ccaccagcgg acgctcaccg ttaccaaaca tcagcatgtc ggctttcgaa
    23941 tccaccagca cggaacggcg cacggtatcg gaccagtaat cataatgcgc ggtacggcgc
    24001 agactagcct caataccgcc gaggatcacc ggtacatctt tccacgcctc tttacaacgc
    24061 tgggtataaa ccagtgtggc gcgatccggg cgcttacccg cgacgttatc cggcgtgtag
    24121 gcatcgtcat gacgtaaacg gcgatcggcg gtataacggt tgatcatcga atccatgttg
    24181 ccagcagtaa caccgaaaaa cagattcggt ttacccagac gcataaagtc gtctttgctg
    24241 ctccagtccg gctgggcgat gatcccgacg cgaaagccct gcgcttccag catacgaccg
    24301 caaatcgcca tcccgaagct tgggtgatcg acatacgcgt cgccagtaac caaaatgatg
    24361 tcgcagctat cccagccaag ttgatccatc tcttcacgag acatcggcaa aaacggtgcc
    24421 ggtccaaaac aggcggccca gtactgcggc caggagaaca ggtcgcgatc cggttggatc
    24481 agggagatag agctcatttt gcttccagaa atgataaaaa aataatcaaa ggccggggat
    24541 tataagccgg aacgaaagag aaatcgaaag gtattccata ctcgccctcc tcgggcgagt
    24601 atgaagatta cggtaccgga ttgaccaaca gttgcccaat cgagccacgg tccgccattt
    24661 ccagcgtctg actgttgaag tagaacggga agtgcgccca ggaaggctga ccgaaataaa
    24721 caagcagctc cacttgtccg tcaacccaaa cggtatcttt ccagcctctg tcttccggga
    24781 acggcatcgc gccattcacg ttacggatct ggaacattac gccttcaata tggaacgctt
    24841 gcggctcgtc cgcgcgtacc gtccagcgtt cccacgttcc ttgctgcgcg gtgacatcaa
    24901 tacggttgac gtcccacagc tgtccattaa tacccgggtc atcacccaga ctgatatcgc
    24961 gactgcgaat tggcgaacca gccatgattt cagttggcag caagcgcatc ggaagactgt
    25021 ctgtgaccag cggcagaagg ccggttgggc gcagcgttag caccagggta gaaaccagaa
    25081 tactggatgg ctcaaagaag ccacgaatac gatcaacaat gctcgccgct tcgccacagg
    25141 tgatcgacac ttcatcgccg ttgctcatat ccaccagaat ctcgcggcgc tcgcccggtg
    25201 ccagcgaaag ttgcttcacc gatacaggag caggcaggaa tccctgatcg ccagaaatca
    25261 catgtaacgg gcgaccatcg ttcatctgta gttgatagcg acgagagttc gacgcgttca
    25321 gcagtcgcaa gcgcacccag ccacgcgaga cttcaacgta cgggctttgt acaccgttaa
    25381 ccagcagcgt atcaccaaca aagccgccgc ttcccggttc gttgtattct ggcgtaccaa
    25441 agttatccag ccgtttatcc tggataatga ccggaaaatc atccacacca taatggttgg
    25501 ggataggcag cgacttgctg acttcatctt ccaccagcca cattccggca aggccgttat
    25561 agacctgctg agccgtgcgg ttgggagtat tggcgtgata ccacagagta gctgcgttct
    25621 ggcgaatggg cagtacgggt gcccagtcag cgtttggcga catcatccgt gccggaccgc
    25681 ccatcagcgg gcctggtacc tgtagcccgg ccaccgtcat tgagacattt tctgtcaggc
    25741 ggttgctgta aataagctta acatcgtcgc ccttccagac gcggatagtc ggccccaggt
    25801 aacgaccatt gattccccag accgacgcgc gtgtccctgg cgtaaatgac cagtgcgcac
    25861 gttgtacagt cataaacagc ggttgcccac ggcgagattc aagtagcggc ggaacgggta
    25921 gcggttgctg ttgcccggct gcgctggcct tcaggggaac agcgcctgca caaagtgcaa
    25981 tccccgatgc ctgaatgaac tgacgccgac tgagtgacat atttgctcca tgtaaaactg
    26041 gctaaagtaa taccgtgcag gaaagtgttc cccgcaaaaa tacagattgc cgacttaaac
    26101 ttttccggcg gcttcgcgtt ctgcgacttc tttatcgagc tcggcgattt tttgttccat
    26161 tatcgaacga caatgggcag ccagctcacg aacctgatct ttgccatact gactgacgtc
    26221 aattggcggc agcatttcga caatcaccag accgttgtgc agtcgattaa gattaatctt
    26281 attcgaagtt gtagagacgc acacaggaat aatcgggacg cccgccgcaa ttgccgcgtg
    26341 aaatgctcca gtcttgaacg gtagcaggcc gcgaccacgg ctgcgggttc cttccgggaa
    26401 catccagatg gaaatacggc gttttttgaa atgattcacg acttccgcaa tggtgccgtg
    26461 agctttagtg cgattgtttc tgtcgatcaa taagttgccg gttaaccagt acaactgccc
    26521 gaagaagggg atccacagca agctcttttt acctaccgtc accgtcggcg gttgcacaat
    26581 gttcgatgct gtcaccatgt catagttgtt ctggtggtta gcgatataga tagcattgcc
    26641 gtagctttca gcatctgcag gtttgcggca ctcaactttc aggccgaaca gcggcgcaag
    26701 acggccaaac atatgcccaa aggtggccac atgtttcggg ttacgcgggc tgaaaaggca
    26761 gtaaatggag ccgaatacac agactaagat gctgtaaatc acggtaataa taagacgaaa
    26821 gatatatagc ataacaccct ctgaaggtct gagagactgg cattgtacgt cacctgaaat
    26881 caggttaggg cattgttaaa agccgctctc tggaaaagcg tattgttaat cgcaacgcat
    26941 gaataaacaa cggtttcaag ggaattttta tcgtttccgg gggttgaata cgctgccgga
    27001 tgacgactta acgtttcatc cggcgttcct tgcaagcggg aggaaacagc gccctccccg
    27061 gcatattact cttcgctatc accgctgctg gcacggcgag gagagtcgat ctcaacacga
    27121 tcgatacgct gcaaaccgcg catcaacgta ccgcggcgtc cacgttcgcc agtgactttc
    27181 tgtagctctt ccggacgcag tttaattttg cgtttcccaa catgaatggt cagcgtgctt
    27241 tgcggcggca gaacgtacag ttgcgccaga ccatcttctc cacgtgcggc ttctgccgat
    27301 ggaatgttga taatcttgtt gcctttgccc ttcgacagct gcggcagatc gcttaccggg
    27361 aacatcaaca tacggcctgc ctgagtgatt gccagcagca tatcggaagc atcttcaatc
    27421 accaccggcg gcataacatg ggcattttcc ggtaaggtga tcaaagcctt acctgcacgg
    27481 ttacgcgcca ccagatcgtt aaaggtgcag acgaaaccgt aacccgcatc ggaagccatc
    27541 agcagtttct gatcgtcgct ttccatcagc atatggtcaa cggtcgcccc aggcggcaac
    27601 gttaatttgc cggtgagcgg ctcgccctga ccacgcgccg acggcagcgt aatcgggtca
    27661 atggcatagc tacgaccggt ggaatcaaca aacactaccg gttggttgct cttacctttc
    27721 accgccgctt tgaagctatc acccgcttta taattcaggc ccggcgcgtc gatatcatgg
    27781 cctttagcgc tgcgtaccca gcccatctgc gacagcacaa tggtgacagg ttcagacggc
    27841 agcatgtcgt gctcgctcat cgctttcgct tcttcgcgtt cctgcaacgg cgaacgacga
    27901 tcgtcaccgt aggcttgcgc gtctgcctgc agttctttct tcagcaggtt attcatttta
    27961 cgctcggaag ccaaaatgcc ctgcaactgg tcgcgctctt tttccagttc actctgctca
    28021 ccgcgaatct tcatctcttc cagtttggca agatgacgca gtttcagttc gaggatcgct
    28081 tccgcctggg tttccgtaag gccaaaccgc gacatcagcg ccggtttcgg ttcatcttca
    28141 ttacgaatga tctcaatcac ttcgtcgata ttgagaaacg ccaccagcaa accttcgagg
    28201 atatgcaggc gcttgaggac tttctccaga cgatagttca gtcggcggcg cacggtatcg
    28261 cggcggaaca ccagccattc ggagaggatt tccagcaggt ttttcaccgc cggacgacca
    28321 tccagaccga tcatattaag gttaatacga tagctctttt ccagatcggt ggtagcgaag
    28381 aggtggttca tcacctgatc catatccacg cggttggaac gcggcacaat caccaggcgg
    28441 gtcgggttct cgtggtcaga ttcatcgcgc agatcgtcaa ccatcggcag ctttttgttg
    28501 cgcatttgcg cagcaatttg ctccagtacg cgcgcacctg aaacctgatg cggcaatgcg
    28561 ctgataacca ccgcgccatc ttctttcttc cacaccgcgc gcatacgcac tgaaccacgt
    28621 ccgttctcgt agattttacg gatctcggcg cgcgaagtga taatttccgc ttcagtcgga
    28681 taatccggcc cctgcacgat atccagcagc tgatcgagcg tggttttcgg ctggtcgatt
    28741 aatgcgattg ccgcctgagc cacttcacgc aggttatgcg gtggaatatc ggtcgccatg
    28801 ccgacggcaa taccggtggt gccgttaagc aaaatgtttg gcagacgggc aggtagcatt
    28861 ttcggctcct gcaaagtgcc gtcgaagttt ggcacccagt cagccgtccc ctgccccagc
    28921 tcgctcaata gcagctcgga atatttcgac aaccgggatt cggtgtaacg cattgccgcg
    28981 aacgatttcg gatcgtccgg cgcgccccag ttcccctgac catcaaccag cggataacgg
    29041 taagagaacg gttgcgccat caggaccatc gcttcataac aggcgctatc gccgtgcgga
    29101 tggtatttac ccagtacgtc accgacggta cgggccgatt ttttaaattt ggcgctggca
    29161 ttcaggccca gttcagacat cgcatacaca atgcggcgct gaacaggttt cagaccatca
    29221 ccaataaacg gcaacgcacg gtccatgatc acgtacatgg agtagtttaa gtaggcgttt
    29281 tccgtaaatt catgtagcgc aaggcgctct gccatatcgc tcattaattc tgattcctca
    29341 acttattcgc cagccttcaa aggggcaata ttgccggaga tactacatta tctgccgcgt
    29401 cgagtcacag gagatcagcg tgaatagcgc cgccggacgt gctccgacgg cgataaaggc
    29461 atcaatgtgc cttgatatac aactctttgc tgtagacata atcctgggga ttatgcagcg
    29521 gaaaaccgcc aacgtagggt ttaagcagtt tgattaacgg ctgatagtag ataggaatca
    29581 gcggtgcctg ttggttgatg atcacttctg cctgctgata taacgcatta cgctttgtcg
    29641 catcagtgat ctgcgtggcc tggtttagta aggcgtcata ctgcgcattt ttccagtgac
    29701 cgacgttttc ttcactatcg cttttgagcg tgttcaggaa gctggaagca tcattgtacg
    29761 tcgcatccca cgactgccga gacagcatga aatcaccggc tcgtcgggca tcaagatagg
    29821 ttttccactc cattgtgcgc agcgtcacct gtgcacccag ccattttttc cattcggaag
    29881 acaacgctat cgcggtcttt tcatgcagat cgtacttgtt gtagaacagc tcaaagcgta
    29941 gcggatgaga ggcgtcgtat cccgcctgtt tcagcaaggc ttttgccatc gcgacgcgct
    30001 cactcattgg cttttgcagt tcatcgaacg tcgtcgcgct aaagcctttt acctctggcg
    30061 gcgtcagcgt ggttgcgggc gttctcaacc ccagtacctt ttgcgcaata agctgtcgat
    30121 caaccgtaag atatagcgcc cgacgcactc gcacatcgtt aaatggcggt ttctcaaggt
    30181 tgaagttgta atattcgctg ttcagacgcg gaataattcg tagctcgcca ggcagtgatt
    30241 tttcaatggc gggaatttgc tgcgccggaa cccaggtgag atcgacctct cccgcgcgat
    30301 agcggttata gccggtgacc gaattatcta gcgccagata ctcaacctgt tgcaatactg
    30361 tatgttgcgc atcgcggtac tttggatttt tgcgtgcagt aatcttttcg ttaactaccc
    30421 actgatcaag cacaaaggca ccgttgtaaa ccatgttctc tggcttactc cagctatcgc
    30481 catgtttagc gatgacatga tgaggaaccg ggaacagcgt cggccaggcg agcatcgtcg
    30541 tgaaccaagg aaccggctgc tcaagcgtaa cttcaagagt acgatcatcc gtcgctttga
    30601 cacccagcga tgtaacatct gctttacccg caacaatagc tgcggcattg ttaatgtgcg
    30661 cctgtgccag atagccagca aaagggcttg ccgttttcgg gtcaaccgcg cgctgccagc
    30721 cgaggacaaa atcctctgcc gtcagaggct gaccgtcgga ccactgcaaa ccgctacgca
    30781 gatggaaaat atagcgcttg ccgccgtcca gtatctccca gcgttcagcc tgagcgggct
    30841 gcacctggcc ttcaccgtcc atccatacca gaccttcaaa cagatccagc acaatctgcg
    30901 cggcagtatt ctcctccacc ttttgcgggt cgagcgtacc tgggtcgcta tgattgttgt
    30961 aacgaaagac ttgttgcggg gcgagcggtg tgttggcggg aacgtcagca gcgtagagag
    31021 gagccgcact taccaggctg accagccata ataaatttcg cgtatacatg gtattcctta
    31081 ccggagaaat tataagccca acaacaatac gcctgtggca ttgttcgctc aaacttatcg
    31141 cgagtgattt ggctcacact ccggtaaaga aaaaagcgag ttgcccccgc ttttccatta
    31201 attaacggat ttcattcaat agttctggat gcttatccag aacacgtaaa agtttgattg
    31261 tggaaggatg aggttgggca ttgccttttt cgtaacgcga aaacgcattt acacctcccc
    31321 caaaaatttc gcttgcctct ttttgggtaa gagagagctt ttttcgaacc ttcactataa
    31381 attcaggtgc cactgtttcg gcattcaccg aagcccgaaa tgcctttact tgcgccatga
    31441 aagcatctga ctcttcttta ttcatgatgc tctcttcgca atggacacaa tataaaccgt
    31501 ggataccttt caatactgtt tttcgtccac ggaaggtgta tggaatatct ttaatgccag
    31561 aaaccatttc tccctggtgg caaaccggac atttcatatt acttctcctt aaacgagacg
    31621 atcagtacgt catgaattac cgtaatttta agataaacct ggcctgtaac aagcctgggt
    31681 ctgtaaacat cctgccagat agtatgatca gagtaggtgg tcatgctttt ataaaagtcg
    31741 ctctctgata atccaatgat aacattacac ataccatcaa aatccaaacc taactcatct
    31801 gcatttaaca gtgcactacg tgttgtacga acttgcccgg cattgacaag ttttttaacc
    31861 tgactcaaac gtgtatgtgg tgtgcgtttt tccataaccc ccgcctccct gttactttag
    31921 ttataaccta aaaggttaat tacagcaatg aaaaagcacc taaaaggtta gttagatgta
    31981 cggagatagt gaccacacaa aacgtattct ttaaggaaag tgattgacca tataagaaag
    32041 tggcgcatta gtagcgccag tttgaagcag gaatttataa gggaagctgg agtcaggcat
    32101 cacgccaacg gcacataaac atccgtttcc agtcgctgct ctgtcacccc ttcggcaaga
    32161 ttggtgtagt gaaacagaat cggtgcttta cgcattttct cgccgcttgt aggcagccag
    32221 tggcgaatga tgccccatac cgtgtgtgaa atatcgtcca gctcgccaac gtggcgggcc
    32281 acggcataac gtccaccggt aagttcacca ttgctcacac cataacgatt atcgggaatc
    32341 ggttcgctaa cgctgccgca gatatcaaag cgaaacgctt ccggtgcggt ggtggcaggg
    32401 tcgtcccagg cgacgccaaa agtctggctt tggttaacag gagaaagccc cgtctcttta
    32461 cgccacatga taaatttcgc cacgctgtag ttgagcaatt cagggctaca gcggtgcggc
    32521 aacatcgcca caggtattga tgggaaatcg ataatgttta catccagtgt caggtttatc
    32581 attttgcctc cctgcgaaat tattcccggg aacgcgtccc gggagcggta acaattacgg
    32641 atttactttg cggatctgtt tgacgtcaat ttcaacagaa ttccagtctt tatcgacttc
    32701 accctgaatc tcaaccgtat ctttcggcgt caccgtcacg ccgttccagc gtttgtggtc
    32761 gatatcaaca ttgatagtac cgctggcatc tttgaacacg tagagatcgt cagagatgcg
    32821 ttcaacgata ttgccgcgca gggttaccca ggtgtcgtca cgcagggatt ttgcgctttc
    32881 tacagtcgtt acgctgccgt tcggcccctg gaatcctccg gcctgacttt gcgttgccga
    32941 tgggccagaa aaaccgccct gctctgctgc catcaccggt gcgctgcaca gggccattac
    33001 tgcgattact gctgcgaatt ttttcatgtt tattactccc tttaatgtct gtttccgagc
    33061 atttaacaag atagtcctta acaacttctt aagggaaaaa aataaaattt agtgctgtac
    33121 agagcgcgtt acaacacggt ttactggcag caaatacggt tatcgcaggg atgaaaaaat
    33181 gcgaatttta ctgatagaag atgacatgct gattggcgac ggcatcaaaa cgggccttag
    33241 taaaatgggt tttagcgtcg actggtttac acaaggtcgt cagggaaaag aggcgcttta
    33301 tagcgcacct tatgatgcgg tgatcctgga tttaacctta ccaggcatgg atggtcgcga
    33361 tattttgcgc gaatggcgag aaaaaggtca gcgtgagccg gtactgatcc tgaccgcgcg
    33421 cgatgcgctg gcggaacgtg tagaagggct gcgtctggga gctgacgatt atctgtgtaa
    33481 accttttgcg ttgatagaag tcgccgccag gctggaagct ctgatgcgcc gaaccaacgg
    33541 ccaggccagc aacgagctgc gccacggtaa cgtcatgctc gaccccggca aacgtatcgc
    33601 cacgctggct ggcgaaccct taacactgaa accaaaagaa tttgccctgc tggaattact
    33661 gatgcgtaac gctggtcggg tactgccgcg caaactgatt gaagagaaac tgtatacctg
    33721 ggacgaagag gtcaccagta atgccgttga agtgcatgtg catcatctgc gacgcaaact
    33781 cggtagtgat tttattcgta ccgtgcatgg tattggctac acattaggtg agaaatgaaa
    33841 tttacccaac gtcttagtct gcgcgtcagg ctgacgctaa tctttttaat tctggcctcg
    33901 gtgacctggc tgctttccag ctttgtcgcc tggaaacaaa caacggataa cgtcgatgaa
    33961 ttgttcgaca cccaactgat gctgtttgcc aagcggttaa gtacgctcga tctcaacgaa
    34021 atcaacgcgg cggatcgcat ggcacagacg ccaaatagat taaaacacgg tcatgttgat
    34081 gacgatgcgc tgacctttgc catctttacc cacgacggca gaatggtcct taatgatggc
    34141 gataacggag aagatattcc ctatagctat caacgggaag gttttgctga cgggcaactg
    34201 gtcggtgaag acgatccttg gcgttttgtc tggatgacct cacctgatgg caaatatcgc
    34261 atcgttgttg gccaggaatg ggaataccgt gaagacatgg cgctggcgat tgttgccggg
    34321 caattgatcc cgtggctggt cgcactgccg attatgttaa tcatcatgat ggtactactg
    34381 ggtcgtgaac tcgcgccgct gaacaaactg gcgctggcac tacgtatgcg tgaccctgac
    34441 tcggaaaaac cactaaacgc gactggcgta cccagcgaag tgcggccact ggttgagtcg
    34501 ctaaatcaac tgttcgcccg cacacatgcg atgatggttc gtgaacgacg ctttacctcc
    34561 gacgcagctc acgaacttcg tagcccgtta acggcgctga aagtgcaaac cgaagttgcg
    34621 cagctctctg acgatgatcc gcaggcgcgg aaaaaagcac tgctccaatt acattccggg
    34681 atcgatcgcg ctactcgtct ggttgatcaa ctgctcacgc tatcgcggct ggactcactg
    34741 gataaccttc aggacgtcgc ggagatcccg cttgaagatc tcctgcaatc gtcggtgatg
    34801 gatatttacc acacggcgca gcaggcgaaa attgacgtgc gactgacact caatgcccac
    34861 agcatcaaac gcaccgggca accgctattg ctaagtttgt tggtgcgaaa tttgctggat
    34921 aacgccgtgc gctacagtcc acagggcagc gtggtagacg tcacgctgaa tgctgataat
    34981 ttcatcgtga gggataacgg ccccggtgtg acaccagagg cactggcgcg aattggcgaa
    35041 cgcttctatc gcccacccgg acaaaccgct accggcagcg ggcttgggct atcgattgtc
    35101 cagcgaatcg ccaaattgca tggcatgaat gttgaatttg ggaatgcgga acaaggtgga
    35161 tttgaggcga aggtaagctg gtaacttctc tgtctatgag agccgttaaa acgactctca
    35221 tagattttac taatagcaaa atataaaccg tccccaaaaa agccaccaac cacaaaccag
    35281 acaaataccg agcgagaata catataccca aaactcgaac atttcccgca taaagagttt
    35341 tcttaagata agaataataa gtggcgtaag aagaaaaaat gctgcaatca ttgcttgcaa
    35401 tatacgatct tcgcctgaaa cggaaggtat actgcaaata ttgcctcccg agtcaagcat
    35461 ccagtcatac ttattgaaag caaaaaaagc atctattaac caataagtaa gcattatgta
    35521 taataatgct tcaaatattg atagtagttg tttttttaac atgtctgatt actcattaat
    35581 caattttttg atatgttctc taatctctaa caacctcata ccataagaga tgaatttata
    35641 atcatctgta tcacgcgcag gcaaattatt aattaaataa ataaaaaacc ccaaatctcg
    35701 ttcaacacca cgattatatc taataccagc cataatatac tgctcattcg tcatatacag
    35761 tgttgccgaa gagggataat caaaaagggc aagatctctt aaatgagtag cagcaatttg
    35821 aaacgtaaaa tcatcttcca ttaggcatgt cgctaactct aactgatttc tggtagttaa
    35881 ttctgaaggg tcaagaccta gagtaatcgc tgcggttcgt atttgtatac ccattgagcc
    35941 aaatgatgtt ttattttcag gcatatctaa caatccaatc attctttttg tttcatatat
    36001 ctgaaactta taattttcag gcatacctcc tgactcaatc caggccacac cgcccagtaa
    36061 ctctggacga atcccagcgg catatgcatg tttaattatt ttgtccctat tatattgtac
    36121 atatgcatct ttataagcat ttaaataata tactcccgaa cggcgacgcg aatcattttt
    36181 tcctaacttc cagagtaaaa cgtccatttc atcccattta cggaacttag tttgtggaat
    36241 tgtacaatag ctatttttat agtgagatcg cttttcatat ttaaaaaacc ctttcttccc
    36301 aacaactata tgattccgta ctcgcgatat ggtgcttcca tgttatattt ttataattaa
    36361 gaacaataac ctcttccggt tgattacccg catgtgtaat tgagtgtgga taacaagtca
    36421 gaatattcga taaatgtgcc ccttgcaatt caattgtgta aaacctttca atacaaccgc
    36481 cagaattaat tctataaaaa ttaaatatag ctgataactc ttcattttca gaaagagcag
    36541 catttaataa aggggaggat ttatctattg gcttgtaaaa ttttatgggg tgatgcacta
    36601 cattatattc tcgacttaaa gaatggtcga actgcaaaac aaaaatttca tccacatgat
    36661 ttatttgata tttattacca
//
LOCUS       DAOFQD010000036        36302 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.036, whole genome shotgun sequence.
ACCESSION   DAOFQD010000036 DAOFQD010000000
VERSION     DAOFQD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.036"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(6..890)
                     /gene="yhdJ"
                     /locus_tag="QBF46_001858"
     CDS             complement(6..890)
                     /gene="yhdJ"
                     /locus_tag="QBF46_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDQ6674046.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRILKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPKQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            complement(976..1272)
                     /gene="fis"
                     /locus_tag="QBF46_001859"
     CDS             complement(976..1272)
                     /gene="fis"
                     /locus_tag="QBF46_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDQ6674047.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1298..2263)
                     /gene="dusB"
                     /locus_tag="QBF46_001860"
     CDS             complement(1298..2263)
                     /gene="dusB"
                     /locus_tag="QBF46_001860"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDQ6674048.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2592..3473)
                     /gene="prmA"
                     /locus_tag="QBF46_001861"
     CDS             complement(2592..3473)
                     /gene="prmA"
                     /locus_tag="QBF46_001861"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDQ6674049.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3485..4936)
                     /gene="panF"
                     /locus_tag="QBF46_001862"
     CDS             complement(3485..4936)
                     /gene="panF"
                     /locus_tag="QBF46_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDQ6674050.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4926..5168)
                     /locus_tag="QBF46_001863"
     CDS             complement(4926..5168)
                     /locus_tag="QBF46_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDQ6674051.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLSGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5277..6626)
                     /gene="accC"
                     /locus_tag="QBF46_001864"
     CDS             complement(5277..6626)
                     /gene="accC"
                     /locus_tag="QBF46_001864"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDQ6674052.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6637..7107)
                     /gene="accB"
                     /locus_tag="QBF46_001865"
                     /gene_synonym="fabE"
     CDS             complement(6637..7107)
                     /gene="accB"
                     /locus_tag="QBF46_001865"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDQ6674053.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8085..9059)
                     /locus_tag="QBF46_001866"
     CDS             complement(8085..9059)
                     /locus_tag="QBF46_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDQ6674054.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRVLPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9211..11151
                     /gene="csrD"
                     /locus_tag="QBF46_001867"
     CDS             9211..11151
                     /gene="csrD"
                     /locus_tag="QBF46_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDQ6674055.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSVLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11456..12499
                     /gene="mreB"
                     /locus_tag="QBF46_001868"
     CDS             11456..12499
                     /gene="mreB"
                     /locus_tag="QBF46_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDQ6674056.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12565..13668
                     /gene="mreC"
                     /locus_tag="QBF46_001869"
     CDS             12565..13668
                     /gene="mreC"
                     /locus_tag="QBF46_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDQ6674057.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13668..14156
                     /gene="mreD"
                     /locus_tag="QBF46_001870"
     CDS             13668..14156
                     /gene="mreD"
                     /locus_tag="QBF46_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDQ6674058.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14165..14758
                     /gene="yhdE"
                     /locus_tag="QBF46_001871"
     CDS             14165..14758
                     /gene="yhdE"
                     /locus_tag="QBF46_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDQ6674059.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14748..16217
                     /gene="rng"
                     /locus_tag="QBF46_001872"
     CDS             14748..16217
                     /gene="rng"
                     /locus_tag="QBF46_001872"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDQ6674060.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16285..20085
                     /gene="yhdP"
                     /locus_tag="QBF46_001873"
     CDS             16285..20085
                     /gene="yhdP"
                     /locus_tag="QBF46_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDQ6674061.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVILALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVNNLTAHITRENPGWQFSIPDTRITMDGNPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLKLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEISTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20241..21686
                     /gene="tldD"
                     /locus_tag="QBF46_001874"
     CDS             20241..21686
                     /gene="tldD"
                     /locus_tag="QBF46_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDQ6674062.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21814..22743)
                     /gene="aaeR"
                     /locus_tag="QBF46_001875"
     CDS             complement(21814..22743)
                     /gene="aaeR"
                     /locus_tag="QBF46_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDQ6674063.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22926..23129
                     /locus_tag="QBF46_001876"
     CDS             22926..23129
                     /locus_tag="QBF46_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDQ6674064.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23137..24069
                     /gene="aaeA"
                     /locus_tag="QBF46_001877"
     CDS             23137..24069
                     /gene="aaeA"
                     /locus_tag="QBF46_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDQ6674065.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24075..26042
                     /gene="aaeB"
                     /locus_tag="QBF46_001878"
     CDS             24075..26042
                     /gene="aaeB"
                     /locus_tag="QBF46_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDQ6674066.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26134..26406
                     /locus_tag="QBF46_001879"
     CDS             26134..26406
                     /locus_tag="QBF46_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDQ6674067.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDALMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26462..26725)
                     /gene="yhcN"
                     /locus_tag="QBF46_001880"
     CDS             complement(26462..26725)
                     /gene="yhcN"
                     /locus_tag="QBF46_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDQ6674068.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27090..27560)
                     /gene="argR"
                     /locus_tag="QBF46_001881"
     CDS             complement(27090..27560)
                     /gene="argR"
                     /locus_tag="QBF46_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDQ6674069.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            27995..28933
                     /gene="mdh"
                     /locus_tag="QBF46_001882"
     CDS             27995..28933
                     /gene="mdh"
                     /locus_tag="QBF46_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDQ6674070.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(28995..30062)
                     /gene="degS"
                     /locus_tag="QBF46_001883"
     CDS             complement(28995..30062)
                     /gene="degS"
                     /locus_tag="QBF46_001883"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDQ6674071.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30152..31519)
                     /gene="degQ"
                     /locus_tag="QBF46_001884"
     CDS             complement(30152..31519)
                     /gene="degQ"
                     /locus_tag="QBF46_001884"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDQ6674072.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31673..32071)
                     /locus_tag="QBF46_001885"
     CDS             complement(31673..32071)
                     /locus_tag="QBF46_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDQ6674073.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRSGAKRD"
     gene            32265..33392
                     /gene="zapE"
                     /locus_tag="QBF46_001886"
     CDS             32265..33392
                     /gene="zapE"
                     /locus_tag="QBF46_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDQ6674074.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTELQGQSDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRSRFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNGETRAQMDELWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYAIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33611..34039
                     /gene="rplM"
                     /locus_tag="QBF46_001887"
     CDS             33611..34039
                     /gene="rplM"
                     /locus_tag="QBF46_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDQ6674075.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34055..34447
                     /gene="rpsI"
                     /locus_tag="QBF46_001888"
     CDS             34055..34447
                     /gene="rpsI"
                     /locus_tag="QBF46_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDQ6674076.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34842..35480
                     /gene="sspA"
                     /locus_tag="QBF46_001889"
     CDS             34842..35480
                     /gene="sspA"
                     /locus_tag="QBF46_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDQ6674077.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35486..35983
                     /gene="sspB"
                     /locus_tag="QBF46_001890"
     CDS             35486..35983
                     /gene="sspB"
                     /locus_tag="QBF46_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDQ6674078.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36025..>36302)
                     /locus_tag="QBF46_001891"
     CDS             complement(36025..>36302)
                     /locus_tag="QBF46_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="YhcL; uncharacterized member of the DcuC family of
                     anaerobic dicarboxylate transporters; probable role in
                     transport of carboxylates across the inner membrane from
                     the periplasm; its encoding gene is not essential for
                     growth and is probably cryptic; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate transporter"
                     /protein_id="HDQ6674079.1"
                     /translation="AIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLAR
                     AVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8689 a         9091 c         9619 g         8903 t
ORIGIN      
        1 ttcctttact ttgcaatgag atcggggtca acttcgctaa cccggcatcg ttttgaccgg
       61 ttgcccgtct ttcttttttt cactttcgcc agttcttccg cggagtaatg cgacgcgaca
      121 tccagccgtc gaagccccat tttgatgtac tcgctgttga tctcaatacc aatgaatttt
      181 cgtccgctgg cgatggctac ggcaccggta gtaaagctac cggcaaacgg gtcgagaacg
      241 atatcgcctg ggttggaaga ggcgagaata atgcgtttca gtaaggcttc cggtttttgc
      301 ttcgggtggt tctcatattc atccattaaa taacgcactc gcggaaaatc ccagacgtta
      361 cccggtactt tttgatgatt gtatggctgt ggaggatttt tgcgataatc gatcaacgcg
      421 cgctgcgatc cggttttggc ttctaccaga atagcatcac cgttgaatgt gtagttcttt
      481 gcgtctttca ccatcatcag gatgggttcg tacatggagc cgtagtgttt tttcgcctgt
      541 actccagaac tgtcatatga ccagacgatg cgacttttga tggtaaaaag cttgcggcac
      601 tggagatcga taaagggcat gttttccgta ctgttcataa tgtacatgct gccctgcttt
      661 ttcagaatgc ggtggcactc tgcaatcact tcaaacagcc agtcgataaa tagatcttct
      721 ttccaggctt cgatcagacc atcaaaattt ttaccgatgt tatacggtgg gtcggcaaag
      781 atcagatcga cgctttcagt aggtagcttt ttaagttcgg caagggcatc accgtgaata
      841 atggtcttag cttcattacc aaaccgggtc ggttcacatc ctgttctcat ggtcactccc
      901 tttgtgacac ctataaaaaa ggcgcttccc catgccgagt agcgcctttt taatcaagca
      961 tttagctaac ctgaattagt tcatgccgta ttttttcaat tttttacgca gcgtaccacg
     1021 gttgatgccc atcatcagcg cagcacgggt ctggttacca cgggtgtatt gcatcaccat
     1081 gtccaacagg ggctgttcta cttcagccag taccagctca tagaggtcat tcacatcctg
     1141 accattcagt tgagcaaaat agttcttcag tgcctgttta accgagtcac gcaggggttt
     1201 ttgggttacc tgatcctgag agttaacggt agaaacggtc agtacgtcag aatttacgcg
     1261 ttgttcgaac atagttctgt cagctcttta tttctgttta cgcaaaattt tcgaagtatg
     1321 cctccaacgc ctccagctgt tcgctggcat cctcaatggc gttgaatgtg cgccgaaact
     1381 ggtcatttgg agcgtgttcc tggagatacc aggaaacgtg tttacgtgca attcggtacc
     1441 cttttgccgg accataaaag tcatgcagtt cccgaacgtg cgcgcaaagc aagcgcttaa
     1501 cctctgccaa aggcagcggg ggcagcaact ccccagtgtc cagataatgc tggatttccc
     1561 gaaagatcca gggtcttccc tgagctgcgc ggcctatcat cagggcatcc gcccctgtat
     1621 agtcgagcac agctctggct ttaagcgggt cagtaatgtc gccattcgcg ataaccggaa
     1681 tggaaacttt ctgcttaact gcccgaatac tgtcgtactc agcttctcca ttgaacaaac
     1741 aggcgcgtgt acggccatga atggtcagag cctgaatgcc acagtcttca gccagttggg
     1801 caatctcttc gcagttacgg tgttccggtg cccagccggt gcgaatcttc agggtaacag
     1861 gaacgtccac tgcattgacg acctcggtaa ggatcgattt aacgacatcc gggtactgca
     1921 agagggctga acctgcgagc ttgcgattca cttttttagc cgggcaaccc atattgatat
     1981 caataatctg ggcaccgctt tccacgttaa tacgtgctgc atctgccatt tctttcggat
     2041 cgctaccagc aatttgcacg gtgcgaatac cgggttcatc aatgtgcacc atccgtaaac
     2101 gagatttgtc gctttcccaa acctgtgggt tagaagacat catctcggat actgtcaatc
     2161 cggctcccat ctcgtagcac aacgtccgaa aaggtctgtc tgtaatgcca gccatgggcg
     2221 ctgcgatcag gcgatttctg agctgatatt gtccgatgcg catgagttaa gaaatgacca
     2281 tactgtgact gcaaggcggc gtatattacg cattttttgc acgagatgaa aggccaaact
     2341 ttgaccaatc ctctgagatg gatcaaagaa ttgcatttaa aatgagcgtg gtgcgataat
     2401 tactcataaa aatcatcata ttagaaaata gtgactaaaa tttacactca aagaaatttg
     2461 agtaagttct caatttttct ttatgaatga aaattttggc acgcaaattt gcgtaaataa
     2521 tcggcaaatt tacgtgcctt ttgtgagctt gctcgcactt cgccccgcgt caccctacgg
     2581 cgatgcgaag gttaattctt acgaccggta atacggcacc actcttcttt ttccacgacc
     2641 gggtccagtg cgaagctatc ggcataagct tcacaaacgc tctctgcctg gcttgccaga
     2701 ataccggaaa ggcccagcaa accgcctgaa accggcagga cgctgattaa cggtgccagt
     2761 tcacgtaatg ggcctgcaag gatgttagcg accaccacgt cggctttcat ttcttctggc
     2821 tgatctttcg gtaagtagag ttccagacgg tcagaaacgc cattacgttc ggcgttatcg
     2881 cggctggcct gaatcgcctg cggatcgata tcaataccaa tggcttttgc tgcacccagt
     2941 ttcagcgccg cgatcgccag aatgccggaa ccacagccaa agtcgatgac tgttttaccg
     3001 gttaaatcga ggctgtcgag ccattgcagg cacagagagg tggttggatg ggtacccgta
     3061 ccaaacgcca gccctggatc taacatcacg ttgacggcgt tttcgtccgg cacatcacgc
     3121 cagctagggc agatccacag tcgttcacca aagcgcatcg ggtggaaatt atccatccat
     3181 tcgcgctccc agtctttatc ttctagttgt tcgattttat gcgcgaagcc tgcgccgagc
     3241 agcggatggt tttccagaat cgccaccacg tcgttcatat cggtttcagc gtcgaacaga
     3301 ccaatcacat cggtgtcgcc ccacaggcgc gtttcgcccg gcagcggctc aaatactggc
     3361 gtatcgtggg tatcctgaaa agtgatagaa acggcacccg cttccatcag cgcatcgcta
     3421 agatcttccg cgttcgcgcc ggtggtgttc agtttcagtt ggatccaagg catggcaaaa
     3481 ctctttattt atcagtagtc aaaacggtag cttgcgggac ggatgtaccg aaacggtttc
     3541 cgaccaggaa agccagcaaa cttagtagta acgagggcac gatagggtgg aagcccaggt
     3601 actgaatatt cagcgtcgcg agtacggcat acagcacgcc gccaacgatc atcgcactta
     3661 gcgcgccttt ggcgttggcg cgttcccagt aaagacccag caccagcggc cacaggaaaa
     3721 cggcttccag cccaccgaag gccagcaaat tcagccagat gatcatttct ggcggcttcc
     3781 aggcggcaag cagcagcaac gcgccgagaa ctaacgtaat taccgccgac atccgcttca
     3841 gacgcgtctc gttttgcatt tgatccggac ggatattcag atagagatct ttaatgatcg
     3901 tagcggaact ttgcagcagt tgggcgttaa ttgtcgacat gatcgcagcc atcggtgcag
     3961 ccaggaagat cccggcggca aacggtggca gcacttttac cattaacgtc gggatcacca
     4021 gatccggtac ggtgagatcg gggatcaccg cccgacctaa cgctccggcc aggtgcatac
     4081 cgaacatcag gattgcgact acaatcgtac cgatgatgat cccccggtgt actgctttgc
     4141 tgtctttata agagatacag cgcaccgcag tatgcggcag gcctataacg ccaaagcaca
     4201 ccagtaccca gaacgacgtc ataaaggcag gcgacagaat atcgtcagcg ccttgtggcg
     4261 taaccagttg cggatcgatg gtttgcaagg tctgtactgc gttgcttaag ccgccagcgg
     4321 catgtaccac gccaataagc agcacaacgg tgccaatcag catcacaagc ccttgcatgg
     4381 tgtcgttcag cacgctggcg cgaaagccac caaaggcggt atataacgca atgctgatac
     4441 caaaaatcag cagcccggtt tcataaggaa tacccgccgc ggtttccagc aggcgcgcac
     4501 cgccgataaa ctgcacggtc atcgcaccaa cgaacgcaac cagcaaactc aaactcgcca
     4561 gccacaccag aagacgactc tggtagcggg caaacagcat atcgttcagc gtcactgcat
     4621 tgtagcggcg cgcaagaatc gcaaacttct tgccgagaat accgagtgaa agccagactg
     4681 caggaagctg aatcatcgcc agcaataccc agcccagccc gtatttataa gcagctcctg
     4741 gcccgccgat aaacgaactg gcactgatat aggtcgcggt gagcgtcatc gccagcacaa
     4801 taccgcccat agagcggctg ccgaggaaat actcattaag gaaggtgccg gtgctccgtt
     4861 tacgcatcgc ataaaccgag ataccgaaca ccaccaccag ataggcgacc agcggtagaa
     4921 ttacttcaag ctgcatcgtc atcctccagt gggatatcgc gatagataaa tttcaccatc
     4981 gcccagcaca gtccaataaa cagcagcggc gtcaggatgc aggccatctc aaaccagcgc
     5041 ggaaagccgg taaaaccggg ggcaacgcca gataagtaag cggctactaa ccaaactgcc
     5101 agatacaaaa gggtcagccc cagcgcccag cgcgcctctt tatgggcctg aacaaaacga
     5161 gtgtccattt tttgtccctg atgggtgaag aaagcgggga ttgtacctta tgggggttgt
     5221 cgatccccag taataaaaaa ggccggaaaa tccggccttt tgacgcttta gcagtcttat
     5281 ttttcctgaa gaccgagttt tttctccaga tagtggatgt tagtgccacc atgctggaag
     5341 ttctcgtcat tcatgatgcg gatctgcaga tcaacgttgg ttttgatacc gtcgatgatc
     5401 agctcctgca gcgcattctt catgcgggca atcgccacgt cacggttttc accgtagcaa
     5461 atcagcttac cgatcattga gtcatagtac ggcggtacgg tgtagcccgc gtagatatga
     5521 gactcccaac gtacgccaaa accgccaggt gcgtggaaac gggtgatttt gcccggactt
     5581 ggcaggaagg tgttcggatc ttcggcgttg atacgacatt ccaccgcatg gccgcgaacg
     5641 tgaacttctt cttgcttgat cgacagcggt tgaccggcag cgatacgcag ctgttctttg
     5701 atcaggtcaa cgccggtgat catttctgta accgggtgtt ctacctgaat acgggtgttc
     5761 atttcgatga aatagaactc gccgttttcg aacaggaact cgaaagtacc tgcaccgcga
     5821 tagccgatat caacacacgc tttagcgcaa cgttcgccga tgtagcgacg cagttccggg
     5881 gtaatgcccg gtgctggcgc ttcttcgacc actttctggt ggcggcgttg catggagcag
     5941 tcacgttccg ccagatagat agcgttgccc tgaccgtcag ccagtacctg aatctcgacg
     6001 tggcgaggat tttccaggta tttctccatg taaaccatat cgttgctgaa agcagctttc
     6061 gcttccgcac gggtcatgga gatggattgt gccagttcag cgtcgccgcg cactacgcgc
     6121 ataccgcgac cgccgccgcc gccggaggct ttgataatca ccggataacc aatgcgttta
     6181 gcaatggcac ggtttttatc catatcgtcg cccagcgggc cgtcagaacc cggtacgcaa
     6241 gggacgcccg cttttttcat cgccgcgatt gcggatactt tgtcgcccat caggcgaatg
     6301 gtgtctgctt tcgggccgat gaagataaag ccggagcgtt caacctgctc agcaaagttg
     6361 gcgttctcgg agaggaagcc gtaacccgga tggattgcta ctgcgccggt gatttcagcg
     6421 gcgctgatga ttgccgggat gttcagataa ctttttactg acggagcagg gccgatacag
     6481 accgtttcat ctgccagtaa tacgtgtttt agatcgcgat ccgcgctgga gtgcacagcg
     6541 acagtcttga tgcccagttc tttacaggca cgaagaatac gcaatgcaat ctcgccgcgg
     6601 ttggcaataa caattttatc cagcatgttc gcctcgttac tcgatgacga ccagcggctc
     6661 gtcaaattct accggttgtc cactttcgac cagaattgct ttcacggtac cggatttgtc
     6721 cgcttcgatc tggttcatca ttttcatggc ttcaacgatg cacagggtat cgcccacgtt
     6781 gactttctga cccacttcga tgaacgcttt tgcgtccggg cttggggtgc ggtagaaagt
     6841 accaaccatc ggggagcgta cgatgtgacc actgatttcc gctgctgctg gcgcttccat
     6901 ggaaggaacg gtcgccggag cggctgcgtt agattgagct ggctgctgca tcattggtgc
     6961 agcgtaagct tgttgcatca cagggaaact tgcggcagga gctgcacggc taatgcgtac
     7021 tgactcttcg ccttcagaaa tttccagttc ggagatgcct gattcttcaa ccagctcgat
     7081 cagtttttta atcttacgaa tatccatgag tgggttccgt actctttgtt tagtgtgatt
     7141 gtgacaggcg tttcaccgcc gtctgtaaag cgtatgcccc gacagctgta tgcatagcga
     7201 taaattccag caggccggga cgccggtcta ttttgcccca gagtgtcaat gttagacttg
     7261 acggacattg tgcagcgatc tgctgctatc ctccggcaaa aaacaaaata taccttcgtt
     7321 gcgccaatgt caccttttcc gcagcgcaaa aactgcgtca gggaggacga ggtcgcacat
     7381 tataacgatt tcgtagcaat tggcagctaa atactggtct tatcagggaa gataatcaac
     7441 agctaacatg taaataacct tcaacaccgt gtaatttgca acaagccgca caattcacga
     7501 aattagcgcc atcatcgacg gaacttctta taacgtaagg tgaaaagctg taaggccagc
     7561 cctgcgaaga tgaggggctg caatgagata atcttcacag accacaaata atgtagggat
     7621 gccaggatcg cgacaagata gacgacatta cgcagcggtt gctggggatt acccaatttt
     7681 cactgcatcg tctggataga tgtgaatgct aaagcacgca aaattaaccg attgacaatg
     7741 ctcagcatta aataggttcg agtgattaat ttgtagtcaa acaaaatcag attattcacc
     7801 ccatggtacc agaaataagt aaggggctaa gcgtagcgtc gcccaggcaa agcaccataa
     7861 acctaacagg cgacggaggc gtatcaataa tagctgttta gtggagtatc aatgcgcatc
     7921 aatttgtggt cagtcgaaat catgactttc cagtttggat gatgttgtat aacttcctga
     7981 ccaaggtgtt cgacgaaagc tgtccggcta accgcttttc ccgttaaccg attgaataat
     8041 gatttcaaaa tgagctccct ctcttattat tccctgctaa atggttagtt aaccttcacc
     8101 agcgtgcgac cctggatctg gttattaatg atggcctcgg caaagttcgg tgcctctgac
     8161 agagatatct cttttgccgc ctgggtatag aatgattccg gtaaatcggc gaccagtcgc
     8221 tgccaggctt gtgcgcggcg ttctggtggc gtcattactg aatccacccc ttgcaaacgg
     8281 acattacgca gaataaatgg catgaccgtg gttggcagag taaaaccacc cgccagacca
     8341 caggccgcca cgcagccgcc gtaattcatt tgcgccagca cttttgccag cactttgtcg
     8401 ccaacggtgt caattgcccc agcccagact tgtttttcca gaggacggga ttcggcaaac
     8461 tcatcacgag ggagaacacg gctagcacct aaacttttca gatattcatg ggtactttcg
     8521 cgaccggaaa cggcaacgac ctgataaccc aacttatgca gcagcgccac ggcggtactg
     8581 ccgacgccac cactggcacc cgtcacgaca atctccccgt cctgcgggcg aacaccggca
     8641 tcttccagcg ccatcacaca cagcatggcg gtaaaaccgg cagtaccgat aatcattgct
     8701 ttacgcgcgt ccagcccttg cggcatggca accagccagt cacctttcac tcgcgcctgc
     8761 tccgccagcc caccccagtg gttttcacca acgccccagc cagtgagtaa cacctcctga
     8821 ccggcatgaa aacgcggatc ttcgctggtg cgtacagttc cggcaaaatc gatcccagga
     8881 atcatcggaa aattacggat gatttttccc ttaccggtaa tcgccagcgc atctttatag
     8941 ttcaggctcg accagtgaac atcgaccgtg acatcgccct ccggcaggcg actttcgtcc
     9001 agagtctgta ctgatgcgag agttttgccg tcctgctgtt ctaaaagtaa cgcctgcata
     9061 agtggtcctc atgtgcatga tggattggaa aataatttct gaagactata ctcgctaatg
     9121 gaaaagcaaa gccgatgaag cgcaataaat tgcgagataa atctgatttg ctagtatgcc
     9181 cgcttcctca ctatcggagt taacacaagg atgagattaa cgacgaaatt ttcggccttt
     9241 gttacgctgc tcaccgggtt aacaattttt gtgactttgc tgggctgttc gctaagtttc
     9301 tacaacgcca ttcagtataa gtttagtcat cgcgttcagg cggtggcgac ggcgattgat
     9361 acccaccttg tgtcgaatga cttcagcgta ttaaggccac aaattaccga attaatgatg
     9421 tcggcagata tcgttcgtgt agacctgctc catggtgata aacaggttta taccctggcc
     9481 agaaatggta gttatcgtcc agttggctcc agcgatctgt ttcgcgaact gagcgttccg
     9541 ttgataaagc atccggggat gtcgttgcgt ctggtttatc aggatccgat gggcaactat
     9601 ttccattcgt tgatgaccac cgcgccgctc acgggggcga ttggctttat cattgttatg
     9661 ctcttcctgg cggtacgctg gttacaacgg caacttgccg ggcaagaatt gctggaaacc
     9721 cgggctactc gtatcttaaa cggtgagcgt ggctctaatg tgttgggaac catctatgaa
     9781 tggccgccca gaaccagcag tgcgctggat acgctgcttc gtgaaattca gaacgcacgc
     9841 gaacaacaca gccgtcttga tacgctgatc cgctcttatg ccgcccagga cgtgaaaacc
     9901 ggcctcaata accgactctt tttcgataat cagttagcaa cgttactgga agatcaggag
     9961 aaagtaggta cccacgggat cgtgatgatg attcgtctgc cggatttcaa tatgttgagc
    10021 gatacctggg ggcacagcca ggttgaagaa cagttcttca ctctgacgaa tctgctgtcg
    10081 acatttatga tgcgctaccc tggcgcactg ctggcgcgtt accaccgcag tgattttgct
    10141 gcgctgttac cgcaccggac gttaaaagag gcagagagca tcgccggtca gttaatcaaa
    10201 gccgttgata ccttgccgaa caataaaatg ctcgatcgcg acgatatgat ccacattggt
    10261 atctgcgcct ggcgtagtgg tcaggatacc gagcaggtaa tggaacatgc agagtctgcc
    10321 acgcgtaatg cgggattgca gggcggcaat agctgggcta tttacgatga ctcgttgcct
    10381 gaaaaaggac gcggtaatgt tcgctggcgt acgcttatcg agcaaatgct cagtcgcggc
    10441 ggcccgcgcc tttatcaaaa accggcggtt actcgcgaag gtcaggttca tcatcgcgaa
    10501 ctcatgtgcc gcatcttcga tggtaatgaa gaggttagct cggcggagta tatgccgatg
    10561 gtcttgcagt ttggcttatc ggaagagtat gaccgtctgc aaatcagccg tcttattcca
    10621 ctattgcgtt actggccaga ggaaaatctg gcgattcagg ttaccgttga gtcgctgatt
    10681 cgcccgcgtt ttcagcgttg gctgcgcgat acgttaatgc aatgtgaaaa atcacaacga
    10741 aaacgcataa ttattgaact tgcagaggcc gatgtaggtc aacatatcag tcgtttacaa
    10801 cctgttattc gtttagtgaa tgctttaggg gtacgggtag ccgtcaacca ggctggtttg
    10861 acgctggtaa gtaccagttg gatcaaagaa cttaatgttg agttactcaa gctccatccg
    10921 gggctggtca gaaacattga gaagcgaacg gagaaccagc tgctggttca aagcctggtg
    10981 gaagcctgct ccgggaccag cacccaggtt tacgccaccg gcgtgcgttc gcgaagcgag
    11041 tggcagaccc tgattcagcg cggtgttaca ggcgggcaag gggatttttt cgcgtcctca
    11101 cagccacttg atactaacgt gaaaaaatat tcacaaagat actcggttta acctgccgtt
    11161 taatctgttt tcacgtagaa taacgcgcgc tgcgtctcat gggagtgtgc ttgtctgctc
    11221 gccagattgt tgcagcacat atgcagatga atgaccttac gcggttgcaa acaggcgagg
    11281 aatgctgctg atgcattaag cctttctgga ctcaggcaga gatttgtaac aaaggaaacg
    11341 aactgcacta attttcaccg tagcagatga tttttgcgcc ttgtcgctgc tgcgtgtggt
    11401 tggtaaagta agcggatttt cttttccgcc ccagctttca ggattatccc ttagtatgtt
    11461 gaaaaaattt cgtggcatgt tttccaatga cttgtccatt gacctgggta ctgcgaatac
    11521 cctcatttat gtaaaaggac aaggcatcgt attgaatgag ccttccgtgg tggccattcg
    11581 tcaggatcgt gccggttcac cgaaaagcgt agctgcagta ggtcatgacg cgaagcagat
    11641 gctgggccgt acgccgggca atattgctgc cattcgccca atgaaagacg gcgttatcgc
    11701 cgacttcttc gtgactgaaa aaatgctcca gcacttcatc aaacaagtgc acagcaacag
    11761 ctttatgcgt ccaagcccgc gcgttctggt ttgtgtgccg gttggcgcga cccaggttga
    11821 acgccgcgca attcgtgaat ccgcgcaggg cgctggtgcc cgtgaagtct tcctgattga
    11881 agaaccgatg gctgccgcaa ttggtgctgg cctgccggtt tctgaagcga ccggttctat
    11941 ggtggttgat atcggtggtg gtaccactga agttgctgtt atctccttga acggtgtggt
    12001 ttactcctct tctgtgcgca ttggtggtga ccgtttcgac gaagctatca tcaactatgt
    12061 gcgtcgtaat tacggttctc tgatcggtga agccaccgca gaacgtatca agcacgaaat
    12121 cggttcggct tatccgggcg atgaagtccg tgaaatcgaa gttcgtggcc gtaacctggc
    12181 agaaggtgtt ccacgcggtt ttaccctgaa ctccaatgaa atcctcgaag cactgcagga
    12241 accgctgacc ggtattgtga gcgcggtaat ggttgcactg gaacagtgcc cgccggaact
    12301 ggcttccgac atctccgagc gcggcatggt gctcaccggt ggtggcgcac tgctgcgtaa
    12361 ccttgaccgt ttgttaatgg aagaaaccgg cattccagtc gttgttgctg aagacccgct
    12421 gacctgtgtg gcgcgcggtg gcggcaaagc gctggaaatg atcgacatgc acggcggcga
    12481 cctgttcagc gaagagtaat cggatgcagg caggggaagt gtctgtttac cctgcctggt
    12541 ctgatacgag aatacgcata acttatgaag ccaattttta gccgtggccc gtcgctacag
    12601 attcgcctta ttctggcggt gctggtggcg ctcggcatta ttattgccga cagccgcctg
    12661 gggacgttca gtcaaatccg tacttatatg gataccgccg tcagtccttt ctactttgtt
    12721 tccaatgctc ctcgtgaatt gctggatggc gtatcgcaga cgctggcctc gcgtgaccaa
    12781 ttagaacttg aaaaccgggc gttacgtcag gaactgttgc tgaaaaacag tgaactgctg
    12841 atgcttggac aatacaaaca ggagaacgcg cgtctgcgcg agctgctggg ttccccgctg
    12901 cgtcaggatg agcagaaaat ggtgactcag gttatctcca cggttaacga tccttatagc
    12961 gatcaagttg ttatcgataa aggtagcgtt aatggcgttt atgaaggcca gccggtcatc
    13021 agcgacaaag gtgttgttgg tcaggtggtg gccgtcgcta aactgaccag tcgcgtgctg
    13081 ctgatttgtg atgcgaccca cgcgctgcca atccaggtgc tgcgcaacga tatccgcgta
    13141 attgcagccg gtaacggttg tacggatgat ttgcagcttg agcatctgcc ggcgaatacg
    13201 gatattcgtg ttggtgatgt gctggtgact tccggtctgg gcggtcgttt cccggaaggc
    13261 tatccggtcg cggttgtctc ttccgtaaaa ctcgataccc agcgcgctta tactgtgatt
    13321 caggcgcgtc cgactgcagg gctgcaacgt ttgcgttatc tgctgctgct gtggggggca
    13381 gatcgtaacg gcgctaaccc gatgacgccg gaagaggtgc atcgtgttgc taatgaacgt
    13441 ctgatgcaga tgatgccgca ggtattgcct tcgccagacg cgatggggcc aaagttacct
    13501 gaaccggcaa cggggatcgc tcagccgact ccgcagcaac cggcgacagg aaatgcagct
    13561 actgcgcctg ctgcgccgac acagcctgct gctaatcgct ctccacaaag ggctacgccg
    13621 ccgcaaagtg gtgctcaacc gcctgcgcgt gcgccgggag ggcaatagtg gcgagctatc
    13681 gtagccaggg acgctgggta atctggctct ctttcctcat tgcgctgttg ctgcaaatca
    13741 tgccctggcc ggataacctg attgttttcc ggccaaactg ggtgttactc atcttgttgt
    13801 attggatcct ggccttgcct catcgcgtaa atgtgggcac aggttttgtg atgggtgcca
    13861 tactggatct gatcagcggc tcgacgcttg gcgtacgcgt attggcgatg agcatcattg
    13921 cttacctggt ggcgctgaaa taccagcttt tccgcaacct cgcattatgg cagcaggcgc
    13981 tggtcgtcat gttgctttcg ctggtggtgg atattattgt tttctgggca gagtttttag
    14041 tgattaacgt ctctttcaga ccggaagtgt tctggagtag tgtagtcaat ggggtgctct
    14101 ggccgtggat tttcttgctg atgcgcaaag tccgtcagca gtttgcagtg caataaaggt
    14161 ttctatgact tctctgtatt tagcttccgg ttctccgcgt cgtcaggagt tacttgcgca
    14221 acttggcgtg acctttgaac gtattgttac gggcattgag gagcagcgtc agccgcagga
    14281 gagcgcgcag cagtatgttg tgcgtctggc gcgcgagaaa gcacgggcag gtgtcgcgca
    14341 aacggcgaag gatctcccgg tgctgggtgc ggatactatc gttatcctga acggagaagt
    14401 gctggagaaa ccgcgcgacg cagagcatgc ggcgcagatg ttgcgcaaat tatcgggtca
    14461 gacccatcag gtgatgacgg cagtggcgtt ggccgacagc cagcacattc tcgattgcct
    14521 ggtggtcacc gatgtgactt tcagaacgtt aacagacgaa gacatcgcgg gctatgtcgc
    14581 cagcggtgaa ccgttagata aagcaggtgc atacggtatt caggggctgg gtggctgttt
    14641 tgtcaggaag ataaatggca gctatcacgc cgtagtcggc ttaccgctgg ttgaaacgta
    14701 tgaattatta agtaatttta acgcactgcg tgagaaaagg gataaacatg acggctgaat
    14761 tgttagtaaa cgtaacgcct tcggaaacgc gagtggcgta tattgatggc ggtattctgc
    14821 aggaaattca tattgaacgt gaggcgcgac gcggaatagt aggcaatatc tacaagggtc
    14881 gtgtaagtcg tgtacttccg ggtatgcagg cggcttttgt agatattggg ctggataaag
    14941 ccgcgtttct tcatgcatcc gacatcatgc cgcacaccga atgtgtggcg ggtgaagaac
    15001 aaaagcaatt cacggtgcgc gacatctcgg aactggtccg tcaggggcaa gatctgatgg
    15061 tgcaggtggt gaaagatccg cttggcacta aaggtgcgcg cctgaccacc gatatcacgc
    15121 tgccttctcg ctatctggtg tttatgccag gggcttctca cgttggggtt tcccaacgta
    15181 ttgaaagcga atcagaacgt gaacgcctga aaaaagtggt cgcagagtat tgcgacgagc
    15241 agggcgggtt tatcatccgt accgcagcgg aaggggttgg cgaggctgaa ctggcctccg
    15301 atgccgctta tctgaaacgc gtctggacca aagttatgga gcgtaaaaaa cgcccgcaga
    15361 cccgttatca gctgtacggc gaactggcgc tggcgcagcg tgttctgcgt gatttcgccg
    15421 acgccgaact ggaccgcatt cgcgttgact cacgcctgac ttacgaagca ttgctggagt
    15481 ttacctcgga gtacattccc gagatgacca gcaagctgga gcattacacc ggacgccagc
    15541 cgattttcga tctctttgat gtcgaaaacg aaatccagcg agcgctggaa cgcaaagtag
    15601 aactgaaatc cggtggttat ctgattatcg accagaccga agcgatgacc accgtggaca
    15661 tcaataccgg agcgtttgtc ggtcatcgca atctggacga caccattttc aataccaata
    15721 ttgaagcgac gcaggctatc gctcgccagt tacggttgcg taatctgggc gggattatca
    15781 ttattgattt catcgatatg aataatgaag atcaccgccg ccgcgtgctg cactcgctgg
    15841 agcaggcgtt gagcaaagac cgggtgaaaa ccagcgttaa tggtttttcg gcgctggggc
    15901 tggtggagat gacgcgtaaa cgcacccgcg aaagcattga gcacgtactg tgtaacgaat
    15961 gcccaacctg ccacggtcgc ggaacggtga aaaccgtgga aacggtatgc tatgaaatca
    16021 tgcgcgagat tgttcgtgtc caccatgctt acgattccga ccgtttcctg gtctatgctt
    16081 ctccggcagt agccgaagcc ttgaaaggcg aagagtcaca ctcgctggcg gaagtggaaa
    16141 ttttcgttgg taaacaggtt aaagtacaaa ttgaaccgct ctataaccag gagcagtttg
    16201 acgtcgtaat gatgtaaaca gatgctgggc cgccatccgg caaagggttt ttgagtcaca
    16261 tttttagcag acaaggagtg acgggtgagg cgattgccgg ggattttact gcttactgga
    16321 gccgcgctcg ttgtgatcgc tgccctgctg gttagcggcc tgcgtattgc tttaccgcat
    16381 cttgacgcct ggcgtccgga aatcctcaac aaaatagaat ccgcgactgg catgccggtt
    16441 gaagccagtc agctctcagc cagctggcag aattttggcc cgacgcttga agcacacgac
    16501 atccgtgcag aactaaaaga tggcggcgaa ttttcggtta aacgcgttat tctggcgctg
    16561 gatgtctggc agagcctgtt acatatgcgc tggcagtttc gcgatctcac tttctggcag
    16621 ctgcgctttc gcaccaacac tcctatcacc agcggtggta gcgatgacag tctggaagcc
    16681 agccacatca gcgatctgtt tcttcgtcaa tttgaccatt tcgatctccg cgacagtgaa
    16741 gtcagtttcc tgacgccatc cggtcagcgc gccgagctgg cgatcccaca actcacctgg
    16801 ctgaacgatc cacgtcgaca ccgtgcggaa ggtctggtaa gcctctccag ccttaccgga
    16861 cagcacggcg taatgcaggt gcgcatggat ttgcgcgatg atgagggctt gttaagcaat
    16921 ggtcgcgtct ggctccaggc ggatgacatc gacctgaagc cgtggctcgg taaatggatg
    16981 caggacaata ttgcgctgga aacggcgcag ttctcccttg aaggctggat gacgatcgat
    17041 aaaggcgatg taaccggtgg cgacgtctgg ctgaaacagg gcggtgccag ctggttgggc
    17101 gagaagcaaa cgcatacgct gtcggtgaat aatctgaccg cgcatattac gcgtgaaaat
    17161 ccgggctggc agttctctat tcccgataca cggatcacga tggacggcaa cccctggccg
    17221 agcggagcat tgacgctggc ctggataccg gaacaggacg ttggcggcaa agacaataaa
    17281 cgcagtgacg aactccggat tcgcgccagt aatctggagc tggcaggcct ggagggcata
    17341 cgcccgctgg ccgcgaaact ttcacctgca ctgggtgatg tttggcgctc cacacaaccg
    17401 agcggcaaga ttaacactct ggcgctggat atcccgcttc aggcggcaga caagacccgt
    17461 tttcaggcat cgtggagcga tctggcctgg aagcaatgga aattattacc gggtgcggaa
    17521 cacttctccg ggacgctttc cggcagcgtt gaaaatggtt tgcttaccgc gtcgatgaag
    17581 caggcaaaga tgccttacga aacggtattc cgtgcgccac tggaaatcgc cgacggccag
    17641 gcaactataa gctggctgaa caatgacaaa ggtttccagc tggatgggcg taatattgac
    17701 gttaaagcca aagccgtcca tgcgcgcggc ggttttcgtt acctgcaacc tgctaacgat
    17761 gaaccctggc tgggtattct ggctggcatc agtaccgatg atggttcaca agcctggcgc
    17821 tatttcccgg aaaacttgat gggtaaagac ctggttgatt atttaagtgg tgcgattcag
    17881 ggcggtgaag cggataacgc gacgctggtt tatggtggca atccgcaact cttcccctat
    17941 aaacacaacg aaggtcagtt tgaagtgctg gtgccgctgc gcaacgcgaa gtttgccttc
    18001 cagccggact ggcctgcatt aactaacctt gatattgaac tggactttat taacgacggt
    18061 ttatggatga aaaccgatgg cgttaatctg ggcggcgtgc gcgcgagtaa tctcaccgca
    18121 gtgatccctg actactcaaa agaaaaactg ctgattgacg ctgacattaa aggtccgggt
    18181 aaagccgttg gcccttactt tgatgagaca ccgctgaaag attctctggg tgcgaccctg
    18241 caagaactcc agctcgacgg cgatgtgaat gctcgcttac atcttgatat cccgctgaac
    18301 ggcgaactgg taaccgcgaa aggtgaagtg acgctgcgta ataacagtct gtttatcaaa
    18361 ccactcgaca gcaccctgaa aaatttgagc ggtaaattca gctttatcaa tggcgatctg
    18421 caaagtgaac cactgacagc aagctggttt aatcagccgt tgaacgtgga tttttccacc
    18481 aaagaagggg caaaagccta ccaggttgcg gtgaatctca acggtaactg gcaaccggcg
    18541 aaaaccggcg ttctgcctga agcggtgaac gaagcattga gtggcagcgt ggcgtgggat
    18601 ggtaaagtgg gcattgatct gccttatcat gctggcgcga cctataacgt agagctgaac
    18661 ggcgatctga ataatgtgag tagtcactta ccttcaccgt tagccaaacc tgcgggtgaa
    18721 ccactagcgg taaacgttaa ggttgatggc aatctcaaca gctttgaatt aaccggacag
    18781 gctggtgcgg ataaccattt caatagccgc tggttgcttg gtcaaaagct gaagctcgat
    18841 cgtgctattt gggcggcaga cagtaaaacg ctcccgccgt tgccggaaca aagtggcgtt
    18901 gaactcaata tgccgccgat gaatggtgcc gagtggctgg ccctgttcca gaaaggcgct
    18961 gcggagagtg tcggtggtgc agcgagtttc ccacaacaca taacgttacg tacgcctatg
    19021 ttgtcactgg gaaatcagca atggaataac ctgagtattg tttcgcaacc gacggcaaat
    19081 ggcaccctgg ttgaggcgca ggggcgtgaa atcaatgcca cgctggcgat gcgtaataac
    19141 gcgccgtggc tggcgaatat caaatatctt tattacaacc cgagcgtggc gaaaactcgt
    19201 ggtgattcaa cgccgtcatc acctttcccg acaacggagc gcattaactt ccgtggctgg
    19261 ccggacgcac aaatacgatg cgcagagtgc tggttctggg ggcaaaaatt cggtcgtatt
    19321 gacagtgatc tcaccatttc tggcgatacg ttaacgctga ccaatggact gattgatact
    19381 ggtttctcgc ggcttactgc cgatggtgaa tgggttaata atccggggaa tgaacgtacc
    19441 tcgctgaaag gaaaactgcg cgggcagaaa attgatgccg ccgcagaatt ttttggtgtc
    19501 acgacgccca tacgccagtc gtcatttaat gtggattacg atttacactg gcgcaaagca
    19561 ccgtggcagc cggatgaggc gacgttgaat ggcatcattc atactcaact gggtaaaggc
    19621 gaaattaccg aaatcagcac gggacatgcc gggcaattgc tgcgcttatt gagcgtagat
    19681 gccctgatgc gtaagctgcg ttttgatttc agagacactt ttggcgaagg gttctatttt
    19741 gactctattc gcagcaccgc gtggattaaa gacggcgtta tgcacaccga cgacacgctg
    19801 gtggatggcc tggaggcgga tatcgccatg aaagggtcgg taaatctggt acgtcgcgac
    19861 ctgaatatgg aagcggttgt cgcaccagag atttctgcga cggtgggcgt ggctgcggct
    19921 tttgcggtta accccattgt tggcgcggca gtgtttgccg ccagtaaagt gctggggccg
    19981 ctgtggagca aagtctccat tttgcgctat cacatttcgg gtccgctgga cgatccgcaa
    20041 atcaacgaag tgttgcgcca accgcgtaaa gaaaaagcgc aatgatttga cgagggcgcg
    20101 taattgcccc aatctcatat gataatcgtt gccaaaggcc aacgagccag aacataaccg
    20161 taggctggat agggcgttca cgccgcatcc ggcagccgta aaaaatcctc tactgcagta
    20221 actaacgagt agcaaaaacg atgagtctta acctggtaag tgaacaattg ctagcggcga
    20281 acggcctgaa acatcaggac ttgttcgcga tcctcggtca actggccgaa cgtcgccttg
    20341 attatggcga tctctatttt cagtcgagct atcacgaatc ctgggtttta gaagaccgca
    20401 ttattaaaga tggttcttac aacatcgatc agggcgttgg tgtgcgtgca atcagcggtg
    20461 aaaaaaccgg atttgcttac gctgaccaaa tcagcctgct ggcgctggaa cagagtgcgc
    20521 aagcggcgcg caccatcgtc cgtgatagtg gtgatggtaa agtacagacg ctgggcgcgg
    20581 tagagcatag cccgttgtat acctcggtag atccgctgca aagcatgagc cgtgaagaga
    20641 agctggatat tctgcgtcgc gtcgataagg ttgcccgcga agcggacaag cgcgtacagg
    20701 aagtgactgc cagcctcagt ggtgtctatg aattaatttt ggttgcggcc accgacggca
    20761 cgctagcggc ggatgtccgt ccgctggtgc gtctttccgt gagcgttctc gtcgaagaag
    20821 atggcaaacg cgaacgcggt gccagtggcg gcggcggtcg ttttggttat gaattcttcc
    20881 ttgccgatct cgacggcgaa gtccgtgcgg atgcatgggc aaaagaagca gtgcgtatgg
    20941 cgctggtcaa tctttctgcc gttgctgcac cagcgggcac catgccggta gtacttggcg
    21001 caggttggcc gggcgtgctg ttgcatgaag cggttggtca cggtctggaa ggcgacttca
    21061 accgccgtgg cacttcagta tttagtggac aggtcgggga gctggtggct tcagaactgt
    21121 gtaccgtggt tgatgatggc acgatggtcg atcgccgagg ttcggtggcg attgatgacg
    21181 aaggtacgcc aggccagtac aacgtgctga ttgagaacgg cattctgaaa ggctacatgc
    21241 aggataaact caacgcgcgt ttgatgggga tgacgccgac tggcaacggt cgccgtgaat
    21301 cctacgccca tctgcccatg ccgcgtatga ccaacaccta tatgctgccg ggtaaatcga
    21361 ccccgcagga aattattgaa tccgttgagt acggtatcta tgcaccgaac tttggtggcg
    21421 gtcaggtgga tatcacctcc ggcaaattcg ttttctccac ttcagaagca tatctgattg
    21481 aaaacggtaa agtaacgaag ccggtgaaag gcgcaacgtt gattggttcc ggtatcgaaa
    21541 ccatgcagca gatttcgatg gttggcaacg acctgaaact ggataacggc gtgggtgtct
    21601 gcggtaaaga agggcaaagt ttgccggttg gcgtgggcca gccaacgctg aaagttgata
    21661 acctgactgt tggcggtact gcgtaataat ttcagtattt tcagaaggat aattaatctt
    21721 tctacgtgca cgaacggtcc cctcgctcct ctggggttag ggtgagggga acccgttggc
    21781 acaggtttgt acaacgtaac agtacaatat gaattacttc tctttcccgc gcccgtgcat
    21841 ctcctgaaac aatttaccga cctcaacaaa ataatccgtc agcgagttga tcacgacctg
    21901 taccttcagc ggcagcttat ctttttcggt atataacgca taaaccgggc gtggatctga
    21961 ctgataacgc ggcagcagga tctccagctc cccacgattg atctcgttga tcacccacat
    22021 cagcggcaca taggcgatcc cggcacccgc cgttagccag cgcaccagcg tcatcggatc
    22081 attagtcaca aatctccctt gcgggatcag gcgagtcgag atcccttccg gtgcaatcag
    22141 ttcaaattca ttgtcgggcc gcacgctgta ttcaagccat gaatggctac ttaaatcggc
    22201 gggtttttcc ggtatgccgt attgtgtgag atagcttttc gcggcgcaca ccaccattgg
    22261 catcgcgcca agacggcggg aaaacaggct ggaatcctgc aacgcgccga cgcggatcac
    22321 cacatccaga ccgtcggcaa tcaggtcggg ggctggaatt ccggtaacca gattgacgct
    22381 caaacctggg tattctttca gcattttggc tgtcagcccg gcgagaacat tttgtgccat
    22441 agttgaagaa cagccaatgc gtagcgtccc gatgggggtg ttattgaagg catacagttg
    22501 ctcatgaaca tcctgcactt catgaagcat acgacggcag ccctggtagt aaattctacc
    22561 ggcttcggtc aggccaatgc tgcgtgtgct acggtttaac agctttacct gcaactcatc
    22621 ttccagtttt gacaccgtct gactgatgga cgaaacgctc atctgtagct gtctggcggc
    22681 ggcggtaaaa gagccaaatt caactacttt ggcaaacacc gacatgcgtt ttagtcgttc
    22741 cattattcac tctgacttaa aagtgattta gatcacataa tatagataac agcataacag
    22801 ttacgctaat atattaaata tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc
    22861 ccttctctgc ggcgacagat gctgaaaata ataacgcctg ctctctctat acaaccaagg
    22921 tcaacatgag tctgtttccc gttatcgtgg tgtttgggct gtccttccca ccgatatttt
    22981 ttgaattgct tttatcactg gcgattttct ggctggtgcg ccgggtactt gtgccaacag
    23041 gtatctacga ctttgtctgg catccggcgt tgttcaacac cgcgctctat tgctgcttgt
    23101 tttatttgat atcgcgactg ttcgtttgag gttgaagtga aaacactaat aagaaaattc
    23161 tcccgtacgg ccatcacggt cgtattagtc attctggcct tcatcgcaat ttttaatgcc
    23221 tgggtctatt acaccgaatc cccgtggacg cgtgacgcgc gctttagcgc tgacgtcgtt
    23281 gcgatcgcgc cggacgtttc tggactcatt acccaggtga atgttcatga taaccagctg
    23341 gtgaaaaaag gacaggtact gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag
    23401 gaagcgcaag ccgatgttgc ttattatcag gtactggcac aggagaaacg ccaggaggcc
    23461 ggacgtcgta accgtctcgg tgtgcaggcg atgtctcgcg aagagatcga ccaggccaac
    23521 aacgtactac aaacggttct gcatcagtta gcgaaagcgc aggcgacccg cgatctggca
    23581 aaactggatc ttgaacgcac ggtgatccgc gcgccagcgg atggctgggt gaccaacctc
    23641 aacgtctata ccggtgagtt tattactcgt ggatcaacgg cggttgcgct ggtgaaacag
    23701 aactctttct atgtactggc ctatatggaa gaaactaagc tggaaggggt gcgccctggg
    23761 tatcgtgcag agatcacgcc gcttggcagt aacaaagtgc tgaaagggac tgttgatagt
    23821 gttgccgctg gggtcaccaa cgccagcagc acgcgtgacg acaaagggat ggcgactatc
    23881 gactctaacc ttgaatgggt gcgtcttgcg caacgtgttc cggttcgtat tcgtctcgac
    23941 aaccagcaag agaacatctg gcctgcgggc accactgcta cagtggtggt cactggcaaa
    24001 caagatcgcg acgaaagcca ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag
    24061 tttggttaat cacgatgggt attttctcca ttgctaacca acatattcgc tttgcggtaa
    24121 aactggcgac cgccattgta ctggcgctgt ttgttggctt tcacttccag ctggaaacgc
    24181 cacgctgggc ggtactgaca gcggcgattg ttgccgccgg tccggccttt gctgcgggag
    24241 gtgaaccgta ttctggcgct attcgctatc gtggcttttt gcgcatcatc ggcacattta
    24301 ttggctgtat tgccggactg gtgatcatca ttgcgatgat ccgcgcacca ttattgatga
    24361 ttctggtgtg ctgtatctgg gccggttttt gtacctggat atcctcgctg gtacgaatag
    24421 aaaactcgta tgcgtggggg ctggctggtt ataccgcgct gatcattgtg atcaccattc
    24481 agccggaacc gcttcttacg ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg
    24541 gtattgtgtg tgcgattatg gcggatctgc tcttttctcc gcgatcgatc aaacaagaag
    24601 tggatcgaga gctggaaagt ttgctggtcg cgcaatatca attaatgcaa ctctgtatca
    24661 agcatggcga tggtgaagtt gtcgataaag cctggggcga cctggttcga cgcaccacgg
    24721 cgctacaagg catgcgcagc aacctgaata tggaatcttc ccgctgggcg cgggccaatc
    24781 gacgtttaaa agcgatcaat acgctatcgc tgacgctgat tacccaatcc tgcgaaactt
    24841 atcttattca gaatacgcgc ccggaattga tcactgatac tttccgcgaa ttttttgaca
    24901 cgccggtaga aaccgcgcag gacgtccaca agcagctcaa acgcctgcgg agagttatcg
    24961 cctggaccgg ggaacgggaa acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc
    25021 gttatcagct tctcaagcgc ggcgttatca gtaacacaaa aatcaacgcc accgaagaag
    25081 agatcctgca aggcgaaccg gaagtcaaag tagagtcagc cgaacgtcat catgcaatgg
    25141 ttaacttctg gcgaaccaca ctttcctgca ttctgggaac gcttttctgg ctgtggacgg
    25201 gctggacttc cggtagtggt gcaatggtga tgattgcggt agtgacgtca ctggcaatgc
    25261 gtttgccgaa tccacgcatg gtggcgatcg actttatcta cgggacgctg gccgcgctgc
    25321 cgttagggct gctctacttt ttggtgatta tccctaatac ccaacagagc atgttgctgc
    25381 tgtgtattag cctggcagtg ctgggattct tcctcggtat agaagtacag aaacggcgac
    25441 tgggctcgat gggggcactg gccagcacca taaatattat cgtgctggat aacccgatga
    25501 ctttccattt cagtcagttt ctcgacagcg cattagggca aatcgtcggc tgtgtgctcg
    25561 cgttcaccgt tattttgctg gtgcgggata aatcgcgcga caggactgga cgtgtactgc
    25621 ttaatcagtt tgtttctgcc gctgtttccg cgatgactac caatgtggca cgtcgtaaag
    25681 agaaccacct cccggcactt tatcagcagc tgtttttgct gatgaataag ttcccagggg
    25741 atttgccgaa atttcgcctg gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg
    25801 cgccgatccc ggttaacgag gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag
    25861 accatgtgat atctgcccgt agcgatgata aacgtcgtcg gtactttggc cagttgctgg
    25921 aagaactgga aatctaccag gaaaagctac gcatctggca agcgccaccg caggtgacgg
    25981 aaccggtaca tcggctggcg gggatgctcc ataagtatca acatgcgttg accgatagtt
    26041 aagtcaaaac cgacgccaaa agcgtcggtt ttttcatggc tatacttagc gatgcacggc
    26101 agaactcgcc gcgaaacgtg acggtggcaa cagatgaata tttatacctt tgattttgat
    26161 gagattgaga gtcaggagga tttttatcgt gactttagcc aaacctttgg tctggcgaaa
    26221 gataaggtac gcgatctcga ctcactatgg gatgcgttaa tgaacgatgt cctgccgcta
    26281 ccacttgaga ttgaatttgt tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta
    26341 atattgctgt ttgatgaggc agaggaagag ctggaagggc atttgcgttt taatgttcgt
    26401 cattagcgca aaaaaaagcc cccgaaccgg gggcaatatc gtcggacaag acgatgaggg
    26461 tttatttgta cagttcagcc gtagcgtgcc aggtgtcacc gctacgagct tcagtaatct
    26521 ggtaggccgt tgcgcctttc tcttccgctt ttttgttcag catttcacgc atatccattg
    26581 gcgaagacgc cacaccactt acggatacgg tcccgattgc ttcacgattt tgtgcttgtg
    26641 cagcatcaat ggagtcggca gcgaatgcac cgaaagagag aacagaaagt acgcttaatg
    26701 cagctacagt ggttttgatt ttcatgattt ttacctcgac ataatctttt agctgggcct
    26761 ttgtttcgtg acccatatca caaaatcaag tatacactaa ttattgagct aattaatacc
    26821 gcgctaactg tttcagttga tactaagtgt taaaaatgtg aattttaata acaaaaaagt
    26881 attaaaaaca cggttattta ttataaatac cagtcttttc aatcagataa tgaattttgc
    26941 ataatgtgct ttttttaccg catgtattca ttgcgtgaat gatatgtcgc ggtaaaacgc
    27001 actattcgtt aaatgaatcg caggggaaag caggggattg agagggataa gcaacatttt
    27061 ccccgccgtc agaaacgacg gggcagagat taaagctcct ggtcgaacag ctctaaaatc
    27121 gcttcgtaca ggtctttgac tgtgaaaccg ttagcagggg tggtaaagat ggtgtcatcg
    27181 ccagcgatgg tgcccagaat accttctgct ttgcccagtg agtccagcag gcgagcaatt
    27241 aactgcgccg cgccagggct ggtatgaatc acgacaacgg catcgttgta gtcgatatcc
    27301 agcaccagat tcttcaatgg actggaggtg gttggtacac ccagttcagc tggcaggcag
    27361 taaaccattt ccattttggc attgcgtgta cgtacagcac caaacttggt caacatccgc
    27421 gagactttag actgattaat attgtcaaag ccttgctcct gcaacgcggc gacgatttcg
    27481 ccctgggagc taaatttctc ttctttaagt aatgctttaa atgctttaac tagttcttct
    27541 tgcttagccg agcttcgcat aagtcacccg atatggtggt tgatacaaca ttattgtgca
    27601 tacagatgaa tttttatgca aacagtcagc cctgaagaag gctgaaataa tgttatgaaa
    27661 gagcgggatt ttatcaaatt tcgttattga gaaacatgcc tgcgtcacgg catgcaaatt
    27721 ctgcttaaaa gtaaattaat tgttatcaaa ttgatgttgt tttggctgaa cggtagggta
    27781 tattgtcacc acctgttgga atgttgcgct aatgcataag cgactgttaa ttacgtaagt
    27841 taggttcctg attacggcaa ttaaatgcat aaacgctaaa cttgcgtgac tacacattct
    27901 tgagatgtgg tcattgtaaa cggcaatttt gtggattaag gtcgcggcag cggagcaaca
    27961 tatcttagtt tatcaatata ataaggagtt taggatgaaa gtcgcagtcc tcggcgctgc
    28021 tggcggtatt ggccaggcgc ttgcactact gttaaaaacc caactgcctt caggttcaga
    28081 actctctctg tatgatatcg ctccagtgac tcccggtgtg gctgtcgatc tgagccatat
    28141 ccctactgct gtgaaaatca aaggtttttc tggtgaagat gcgactccgg cgctggaagg
    28201 cgcagatgtc gttcttatct ctgcaggtgt agcgcgtaaa ccgggtatgg atcgttccga
    28261 cctgtttaac gttaacgccg gcatcgtgaa aaacctggta cagcaagttg cgaaaacctg
    28321 cccgaaagcg tgtattggta ttatcactaa cccggttaac accacagttg cgattgctgc
    28381 tgaagtgctg aaaaaagccg gtgtttatga caaaaacaaa ctgttcggcg ttaccacgct
    28441 ggatatcatt cgttccaaca cttttgttgc ggaactgaaa ggcaaacagc caggcgaagt
    28501 tgaagtgccg gttattggcg gtcactctgg tgttaccatt ctgccgctgc tgtcacaggt
    28561 tcctggcgtt agttttaccg agcaggaagt ggctgatctg accaaacgta tccagaacgc
    28621 gggtactgag gtggttgaag cgaaagccgg tggcgggtct gcaaccctgt ctatgggcca
    28681 ggcagctgca cgttttggtc tgtctctggt tcgtgcactg cagggcgaac aaggcgttgt
    28741 cgaatgtgcc tacgttgaag gcgacggtca gtacgcccgt ttcttctctc aaccgctgct
    28801 gctgggtaaa aacggcgtgg aagagcgtaa atctatcggt accctgagcg catttgaaca
    28861 gaacgcgctg gaaggtatgc tggatacgct caagaaagat atcgccctgg gcgaagagtt
    28921 cgttaataag taattgatta gcgaataata aaaaaccgga gcacagactc cggtttttgt
    28981 tttgagcact cgacttaatt ggttgccgga tattcctgaa tggtgacctg cagcgttaac
    29041 tgcttatcat cacgcatcac tacaaccggg atcaccgaac cagggcgaat ttccgccacc
    29101 tgatccatcg tctccagagc agagatggcc ggtttgttat ccaccgaaat aatcagatcg
    29161 ttgacctgaa tacccgcatt cgccgccggg ccgtcaggtg acacttcatt aaccacgatc
    29221 ccttgcagtt gatctatacc accgccctgc gcgtgcagtg gtgcgatctc acgtccgccg
    29281 ataccaatgt agccgcggat cacgcgacca tcgcggatca gcttatccat aattttggtt
    29341 gctaactgga aaggaatcgc aaagccgata ccttccggcg tttcgccatc gttactctta
    29401 tcaaacgaca gcgtattaat gcccatcagt tcgcccagcg agttcaccag cgcgccgcca
    29461 gagttaccgt ggttaatgga agcatcggtt tggaggaagt tttgccgccc ggtcgggttc
    29521 agaccgattc gacccgtggc actaataatc ccctgggtaa tggtctgccc gaggttgtac
    29581 gggttaccga tcgccagtac tacgtcgcca atgtgcggta cgcgacgtgc attaattgga
    29641 atggtaggta aaccgccagt ggcattaatt ttaagtaccg ccagatcggt tagagagtca
    29701 gatcccacca gcaatgcttc aaatacacgt ccatcctgta aggcgacgat gatctgatcg
    29761 gcgtcgttga tgacgtgttt attggtgatg atataaccgc gttgatccat gattacaccg
    29821 gatcccaggg tgcggatctc aagctggttg tgagagttgg tgttcaaacc acggttgtaa
    29881 acgttaacca ccgctggcgc ggcgcggcga accgccagat tataactggc aggcgtctca
    29941 tcggtactgt caaattgcgg agtggaaagc gggttaaggc tgcgcagcga aggcatggca
    30001 accagcagaa tagcgccgac aattaatcca atcgcaacgg aacgtaagag cttcacaaac
    30061 atgatggagg cgtcattaaa aaagggaacg gcagcagcat accacgagtt aaccggacat
    30121 cacacgtcag cctgatgccc ggtttacgac attaacgcat cagcagatag atgctttcat
    30181 tgccgcgtac aatttgcagg gcgatgatgg ctggttttgc cgccagcact ttacgcattt
    30241 cagcaatcga gttcacccga tcgcggttga cgccaatgat cacatcgtct ttttgcaagc
    30301 cagcctgagc agctgggctt cctttgacaa cttcatcgat tttaatacct ttgccgccat
    30361 cttttagctg accatcgctc aacgttgcac cttccagcgc tggcgtgatc atttcagcgc
    30421 tggccgacga agaggtgctg gtatcgagcg tcacttctac ttccagtggt ttgccgttac
    30481 gcagcaggcc aagtttcact tttgtgcccg gctcggtggt cgcgatacga gagcgcaact
    30541 cagcaaagct attcagcggt ttgccgttga ggctggtaat aatatcgccc gctttgacgc
    30601 ccgctttcgc cgagccagaa cctggcaaca cttcgctgac aaacgcgcca cgctgcacgt
    30661 caaggttgaa ggctttggcg atatcggcac tcatctcggt gcctttgatg cctaacaaac
    30721 cgcgtttgat ttcaccaaag tcgataagct gctgcgccag tgttcgcgcc atattactgg
    30781 ggatggcaaa tccaattccg acgctcccgc cgccaggcgc aaggattgca gtgttgatgc
    30841 caattaactc accgttaagg tttaacagtg caccgccgga gttaccgcgg ttaatggaag
    30901 catctgtctg gataaagttt tccagacctt caagattcaa cccgctgcgt cctaatgcgg
    30961 aaacaatgcc agaggtggcg gtttgcccaa ggccaaacgg gttaccgacc gccacggcaa
    31021 aatcaccgac gcgcaattta tcggagtcgg caatagcgat ttgcgttaat ttgctcgggt
    31081 tttgaatttg caacagggcg atatcgctct ggtcatcgct accaatcagt tttgcatcaa
    31141 actcgcgccc atcattgagc tgaatactga ttttctgtgc ctgattaatc acatggttgt
    31201 tggtcagcac atagccttta ctggcgttga tgatgacacc ggagcctaaa ccttcgaagg
    31261 gttgtgcagg ttgatccggt aaatcatcac caaaaaactt tttgaattct tccgggattt
    31321 tctgtccctg actggccgtt ccttccaccc gtacgctcac cactgccgga agcacttttt
    31381 ccagcattgg agccagactg gggagggggg cctgatcggc aacctggcct ggaatcgacg
    31441 cgacggcctg aaatgacgcc gagagagtta acccgacact taacgctaat gcactcaaca
    31501 gctgggtttg ttttttcatt attcctgctc tcgtacctga atgataagaa aagagattca
    31561 aaacagtttg atgttattga attttcagac ggctaacaat gagacaccag attaaatgat
    31621 agtcggggag ggagagaaga ggtgggcgca atggctgcgc ccgaaaaata aattagtcgc
    31681 gttttgcgcc actacgcagc aggccggatg cgccttcaga atagtcgcga ggcatctgca
    31741 ccggtgcctg atcgttgctg gcttcagact ctgccagacg attacggaac gggtttgctt
    31801 cagcagacag ttccggcagc aggctgctgg agcttttcgc catatgctga taaagctggc
    31861 gatagtcgtg cgccatggta tccagtaatt ccgcgctgcg ggcaaagtgg ctaaccagct
    31921 cttcgcgata ctcgtccagt tcagctttat tcttttccag ttcgtactgc aacgcctgtt
    31981 gctggcgtag tttacgatta ccaaaacgca tggccacagc accaataatg atgccgacga
    32041 ctaacccaat tagcgcatat tcccaggtca tgaacatctc ccgttgtctt ttgtttccgt
    32101 agggtgttgg cttcaggctc catgcctgcg gctgattatg ccactataac cgttaattcc
    32161 acagaagtgg aatcccgact gcatatcgcg tagtgtagaa cggccttttt ttcgtcaacc
    32221 gtgaacaacg gcgcaccgat tattcaagga ataacaataa gatcatgcaa agcgttaccc
    32281 caacatcgca atacctgaag gcgctcaatg aaggcagcca tcaacccgac gacgttcaaa
    32341 aagaggccgt cagccgcctg gaaattattt atcaggaact catcaatagc acgccaccag
    32401 cccccaggac gagtgggcta atggcgcggg tcggtaagct gtggggtaaa cgcgaagaca
    32461 caaagcatac gccagtgcgt ggcttatata tgtggggcgg tgtaggacgc gggaaaacct
    32521 ggctgatgga ccttttctat caaagcctgc cgggagagcg gaaacagcgc ctgcactttc
    32581 accgttttat gctgcgggtg cacgaagagc tgaccgaatt acaaggccaa agcgacccgc
    32641 tggaaattat tgccgatcgc tttaaagcag aaactgacgt gctctgtttt gacgaatttt
    32701 ttgtttctga tattaccgat gccatgctac ttggcggtct gatgaaagcc ctgttcgccc
    32761 gcggtattac cctggtagcg acgtcaaata ttccgccgga cgaactttat cgaaatggcc
    32821 tgcaacgttc gcgttttctg cctgcaatcg atgccattaa acagcattgt gatgtaatga
    32881 acgtggacgc tggtgttgat tatcgactgc gtacactcac tcaggcgcat ctgtggcttt
    32941 cgccactcaa cggtgaaacc cgggcgcaaa tggatgaact atggttggcg ctggcggggg
    33001 tgaaacgaga aaattcaccg acgttagaaa tcaaccatcg gccattggcg acaatgggcg
    33061 tcgagaacca gacactggcg gtctctttta ctaccttgtg cgtcgatgcc cgcagtcagc
    33121 acgactatat tgcgctctca cgcctctttc atacggtcat gttgtttgat gtaccagtta
    33181 tgacgcggtt gatggagagt gaagcgcggc gctttattgc gctggtggat gagttttacg
    33241 agcgccatgt caaattagtg gtgagtgcag aagtgccgct gtatgcaatt tatcagggcg
    33301 agcggctgaa atttgagttc cagcgttgcc tgtcacgtct gcaagagatg caaagcgaag
    33361 agtatctgaa gcgcgagcat ttagcgggtt aaaacctgcc acaaatcaca aaaaggggtc
    33421 gatctttgac cccgacttct ctataatcct gcgaccccac gttacaagaa agtttttttc
    33481 ccaaaacttt ttgtgtgctg gcataggcta ttcgaagggg taggtttgcc ggactttgtc
    33541 gtgtgaacct caacaattga agacgtttgg gtgttcacca acgtgtaact atttattggg
    33601 taagctttta atgaaaactt ttacagctaa accagaaacc gtaaaacgcg actggtatgt
    33661 tgttgacgcg accggtaaaa ctctgggccg tctggctact gaactggctc gtcgcctgcg
    33721 cggtaagcac aaagcggaat acactccgca cgtagatacc ggtgattaca tcatcgttct
    33781 gaacgctgac aaagttgctg taaccggcaa caagcgtact gacaaagtgt actatcacca
    33841 caccggccac atcggtggta tcaaacaagc gacctttgaa gagatgattg ctcgccgtcc
    33901 tgagcgtgtg attgaaatcg cggttaaagg catgttgcca aaaggcccgc tgggtcgtgc
    33961 tatgttccgt aaactgaaag tttacgcggg taacgagcac aaccacgcgg cacagcaacc
    34021 gcaagttctt gacatctaat cgggattata ggcaatggct gaaaatcaat actacggcac
    34081 tggtcgccgc aaaagttccg cagctcgcgt tttcatcaaa ccgggcaacg gtaaaatcgt
    34141 aatcaaccaa cgttctctgg aacagtactt cggtcgtgaa actgctcgca tggtagttcg
    34201 tcagccgttg gaactggtcg acatggttga gaaactggac ctgtacatca ccgttaaagg
    34261 tggtggtatc tctggtcagg ctggtgcgat ccgtcacggt atcacccgcg ctctgatgga
    34321 atacgacgag tccctgcgtt ctgaactgcg taaagctggc ttcgttactc gtgacgctcg
    34381 tcaggttgaa cgtaagaaag tcggtctgcg taaagcacgt cgtcgtccgc agttctccaa
    34441 acgttaattg gcttctgctc cggcagaaaa caatttgcga aaaaacccgc ttcggcgggt
    34501 ttttttatgg ttaaattctg aatcagcgta aaaaatggaa agttactttt tgctgccgtc
    34561 tgacagacag gcaaaataaa aaccacatcg ccaataaggg actaagtcaa ctatttcaga
    34621 ctaaagcgca tctctttttc cccatttccg gcatcgactc accacaaagg tcgcaaaatc
    34681 tggtaaacta tcatccaatt ttctgcccaa atgtcgggta ttgctcattt tttgtttgat
    34741 tttcgaacaa agagagtcgt tccttactgg gtaacacaac ttctgactgg ccacctggtg
    34801 gctggtagca gtaaaaattc tgactatacc tggaggtttt catggctgtc gctgccaaca
    34861 aacgttcggt aatgacgctg ttttccggtc ctactgacat ctatagccat caggtccgca
    34921 ttgtgctggc tgagaaaggt gtaagtttcg agatcgaaca cgtggaaaag gacaatccgc
    34981 ctcaggatct gattgacctc aacccgaatc agagcgttcc gaccctggtg gatcgtgagc
    35041 tgaccctgtg ggaatctcgc atcattatgg aatatctgga tgagcgtttc ccgcatccgc
    35101 cactgatgcc tgtttacccg gtagctcgcg gtgaaagccg tctgtacatg catcgcatcg
    35161 aaaaagactg gtacacgctg atgaacacca tcatcaacgg ttcagcttct gaagcagatg
    35221 ccgcacgtaa gcaactgcgc gaagaactgc tggcgattgc gccggtcttc ggtcagaagc
    35281 cgtacttcct gagcgatgag ttcagcctgg tcgattgcta ccttgctccg ctgctgtggc
    35341 gtctgccgca actgggcatc gagttcagcg gcccgggtgc gaaagagctg aaaggctata
    35401 tgacccgcgt ctttgagcgt gactctttcc ttgcttcttt aactgaagca gaacgtgaaa
    35461 tgcgtctggg ccggagttaa tctgtatgga tttgtcacag ctaacaccac gtcgtcccta
    35521 tctgctgcgt gcattctatg agtggttgct ggataaccag ctcacgccgc acctggtggt
    35581 ggatgtgacg ctccctggcg tgcaggttcc tatggaatat gcgcgtgacg ggcaaatcgt
    35641 actcaacatt gcgccgcgtg ctgtcggcaa tctggaactg gcgaatgatg aggtgcgctt
    35701 taacgcgcgc tttggtggca ttccgcgtca ggtttctgtg ccgctggctg ccgtgctggc
    35761 tatctacgcc cgtgaaaatg gcgctggcac gatgtttgaa cctgaagctg cctacgatga
    35821 agatgccagc atcatgaatg atgaagaggc atcggcagac aacgaaaccg ttatgtcggt
    35881 tattgatggc gacaagccag atcacgatga tgacactcat cctgacgatg aacctccgca
    35941 gccaccacgc ggtggtcgac cggcattacg cgttgttaag taatacaaaa caggccctgg
    36001 tggcctgttt tgtcttttaa tggcttaata aaatagcgtg attgtggcaa tcatattcac
    36061 cacgaaaccg actgccatgg ggatcgctgt ccgcttcacc accgcaaaag gcgaaacgcc
    36121 tgcaattcct gacgtaacga ccaccaccgc agtaatcggc gaaaccgcgc gcgctagcgt
    36181 cgtggcaaaa tgcatcggca taatcattac aaccgctggt acatgtagtc cggctgcgat
    36241 attcggaata agactggcaa atgacataaa cggcgcattg cctgagccca tcacaatggc
    36301 ac
//
LOCUS       DAOFQD010000037        36225 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.037, whole genome shotgun sequence.
ACCESSION   DAOFQD010000037 DAOFQD010000000
VERSION     DAOFQD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.037"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(249..461)
                     /locus_tag="QBF46_001892"
     CDS             complement(249..461)
                     /locus_tag="QBF46_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674080.1"
                     /translation="MSETKQAYLMKFRKCSSFDTLEKVFERLCEKNAGIASLEISGAY
                     DHRKAELTMKKLYDKVPASVWTLVRQ"
     gene            676..1857
                     /locus_tag="QBF46_001893"
     CDS             676..1857
                     /locus_tag="QBF46_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6674081.1"
                     /translation="MNSKPVTRQFEDSDLHQELVTFEVPNNDLKELIFYFSHMKYNTA
                     KTYLQWLRSWNEWYQANAGKEGNEAWPASSLPVTEPPLLAYLDYLQGSLSHSSIKGCL
                     HALNSIHRKALDRPGIITSKVKSILASLEQAEAREQKVTRQATPFLVSDLKALIKAHG
                     TTQSVRKLRDLCIIWTGFETLLRSAELRRIRMQDLVLNEQTGSFTLTVYRTKSTVSTL
                     LTYHLTPHLTATLIRLMDMVKRDQQSHPKDYLFQAVNYQDSGYMPPGWGLRSKGNEIN
                     TLLKNHNMPYRPTRPPIGKNGKPIIVDDEGMLSKNTLLRAFEAFWDELHPQEAGTRCW
                     TGHSVRVGGAIELANAGYTHLQIMEMGNWSNPEMVSRYIRNIDAGKKAMTKFMREALD
                     E"
     gene            complement(1882..2226)
                     /locus_tag="QBF46_001894"
     CDS             complement(1882..2226)
                     /locus_tag="QBF46_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674082.1"
                     /translation="MELGIMVCIVLLLSLAAVWISNKTVGLLEIHYFKRPLSMEYAAW
                     LRVMCAGLLFIFIQMVPAFTFLYTLKLVALLNLGVQTMKLNGVYKFKRTGMMPPKDAP
                     HDLGRFNPFRKK"
     gene            complement(2162..3070)
                     /locus_tag="QBF46_001895"
     CDS             complement(2162..3070)
                     /locus_tag="QBF46_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674083.1"
                     /translation="MQWISTTSQKLDERLYRVCVWVKKYHEDAINRVVLTVDLSKARH
                     DPIEDQVRAELLCGHYFLMRKENNAKSAPADQNFEFLPDGRNLSWQTSTPGLQHLLLN
                     SNVPESLRPLADYIQMRLAKLTMVPMSGTIMKAALEDSISWVKVDLRYYWQYELVNSH
                     LGPVQVTHKALVRFGRLAKHDENASAIRMLRQRLSSPFIQEFEMPGEELKRKQKIMNT
                     VDVKMLFHTHYPGQKLLLARYANGWVLVDCFLFHHIKPKKKAKNKTKAGKSETQNIKS
                     TGSEDHGTGHYGVHRVVTLTGSGVDQ"
     gene            complement(3081..4040)
                     /locus_tag="QBF46_001896"
     CDS             complement(3081..4040)
                     /locus_tag="QBF46_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDQ6674084.1"
                     /translation="MASEDLLFYRDRILERIEKLRSFLNDHQPVMAELMTVGTVTRHE
                     ERLKEVNPIEVSTISDASVDEIIRVIQDFCIDDIDVLSRNSTKVTTKYPGLIIVPEGA
                     EQLSGIICDINDAKSDFASAMKRVNSEKHIRFKEVHRKLPGLVTAHSTRKILFVEEQL
                     KKVTFAWRLNRNQVKTNASDLIAMLDKRRLVAVKSPVTTDLTVVANIDRAKALLERKV
                     LRDGEGYRLCRTNTFPVPIAHLFTYRPEGKERGSSKYAETDYKVVKASLPIFGVGQKP
                     TIKRLQDWVPAQDEALSNGRRSNHSYTELVPGADLGIFIMRKV"
     gene            complement(4129..4404)
                     /locus_tag="QBF46_001897"
     CDS             complement(4129..4404)
                     /locus_tag="QBF46_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674085.1"
                     /translation="MHLLVEGKGVIPDVSVSDLGNVQNIRNELIGLGWWVKFGVKQDW
                     QRINLQRQPVSFGLLNDLNRCFRHDGQQVEPLPLYTTGNPGELRKVL"
     gene            complement(4622..5014)
                     /locus_tag="QBF46_001898"
     CDS             complement(4622..5014)
                     /locus_tag="QBF46_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674086.1"
                     /translation="MVFWIIISFMFLSAMFMTGHLLNKVVLQPVWLCIWSLPWLGFLL
                     KVRVTEMDKGLVNTLQLGDLPVFRWSRSVVPGTWSVDRINGRYRLNRIGTDGLTVHYP
                     WRLYYQPLFLTPDKQISDINGREHERKF"
     gene            complement(5094..5630)
                     /locus_tag="QBF46_001899"
     CDS             complement(5094..5630)
                     /locus_tag="QBF46_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674087.1"
                     /translation="MKSKIHSSGTSGTKRVLKTDIALPLLCWVFTSPFSNWTDKFFTG
                     TEVPEGSLPGLEQAPEAIFRFVLNDEGFDVGFDAVGMDLCCFSIPLSTMPTKNLDDEE
                     TLSRLTGDVIHGVLLSLPEYIEMPDRLVYQLTDEVMAFNSHCGNGILHGWTTAQELWR
                     NEILPRTTILMQQTSVIH"
     gene            complement(5704..5961)
                     /locus_tag="QBF46_001900"
     CDS             complement(5704..5961)
                     /locus_tag="QBF46_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674088.1"
                     /translation="MSNTDSQTVPADALCENSSQVKIQLSRSASRALTTAKEILETRG
                     LVSVSTEDLVQACLEENQPLDLASIYLEHRLKQKGTSICEF"
     gene            complement(5969..6559)
                     /locus_tag="QBF46_001901"
     CDS             complement(5969..6559)
                     /locus_tag="QBF46_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674089.1"
                     /translation="MMTESFFDWVVYCITGVILASAFLEGKRTRFPAAFFYGSIVLLV
                     VLIVAGALNWWFYRSKFGSVVVIFAATTLLMTRFRLVIYAFRFRTLVADLQSHVAKGF
                     RIILVLPENEDEKTVLLSKLSKVIHSGTLFYTRTALGPYSGDLLHALGQKHRNGEGYL
                     LLCEQQLPARTWLSTVENGQPEKSIAVNFHSIPDME"
     gene            complement(6614..6883)
                     /locus_tag="QBF46_001902"
     CDS             complement(6614..6883)
                     /locus_tag="QBF46_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674090.1"
                     /translation="MPATTILSNNPVNSVFSNDNSHFTYRHIDFSIELLLSSGTDSQL
                     EYLFLKPAAEVKIDLQRRLASGERYVTTESCDNFSEVDGCMGHDC"
     gene            complement(7296..8252)
                     /locus_tag="QBF46_001903"
     CDS             complement(7296..8252)
                     /locus_tag="QBF46_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674091.1"
                     /translation="MTNSTDNQNYVRAVLAGIGIDFDETEMFISVSHCQSDEVSFTCS
                     ISASELRESAGHYVDTLNDTQLAGLDADALKKRLVYFLEVFDLVSGQYLDISGKHFAT
                     SRFEYDDVCSEILSNSADSAQPGGYDREEYKRLMEVDGQVLIARFALEKFWDTHFIGL
                     INYVSDEITSGLYEVYRTFSDINMAGYTFSEYSYTRRITDELSLHISLKEDDFEEQLT
                     DCYMDETTLPSGKVVLRRNNESIIGIYEGYASKSYFPMVANVRVLDTDGEVVTELYQG
                     VNVSELAGGRIKIHDRQELISEVFANLREFIPASEDKIFDAA"
     gene            complement(8429..9364)
                     /locus_tag="QBF46_001904"
     CDS             complement(8429..9364)
                     /locus_tag="QBF46_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674092.1"
                     /translation="MFNESDDKNFVSAMLKCQLGLNISQEDITIYDKENHFEQLSFKA
                     NVALDDLLFYLDLYISELIKHNAPYSETEVLRTKIKYFLKVYEKSGFQNIRIRGYHNA
                     HSTIDIVDIASLILAGSVPESEHDSIDPVLRKEIYQNRMSVEGKVLIARFALKQFFHS
                     DFGDFILEFEKSISKCLNTSLQIIKSVKNSFNRLGQYQYQRRVKDDLTLHLDLNTDEY
                     PACMPDLYIGFKESEGTTGVYRDDEKIIRLYTGVSSGKDVPVMMTVRFTGCDGSVLSE
                     SSHGTFCSVGPTGRVQVCDRVALVQEAVEELRDVV"
     gene            complement(9437..10357)
                     /locus_tag="QBF46_001905"
     CDS             complement(9437..10357)
                     /locus_tag="QBF46_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674093.1"
                     /translation="MKAIYKIIEAMLVKMGFEGAVIQSVNLETGVIQSLVVKADIPVR
                     TLMPHLRRYLRDCEPLWGHDVDYHPIAQYCSGAALLKAGPVTLETFRFEVSVSTAHLR
                     LVDVEPDCLNEGVNREKLEYVYYRSSNADKDLILGMVEKGHNFINHLHELLRSRLSTA
                     LPLIFAVTEKIAESSVPQVVTSFFSVDHQINAQIVVEPVPVSVADQIISADWNVCSVS
                     GADKFLNHVNRYLSGRVMKRRIYAVIEACDGGSLSLNANLGCFTSPLLVTEYKMVQSH
                     SSSLYRKAINNSLKQVNSYAPEHSDDLLSM"
     gene            complement(10435..11370)
                     /locus_tag="QBF46_001906"
     CDS             complement(10435..11370)
                     /locus_tag="QBF46_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674094.1"
                     /translation="MKIIEKIINAFLISRKHSVKVSNVICLSGTDGKFTGICCDADVS
                     FDFLYSYAPAYSSTFLDIPFPGFEDQDIADCVKCQLDVVKNKRNRSFLIDHIRFPVSS
                     REGFTLTRGDSYDVTECEYNKERLLHLTRQGRFCEDYLTFKDGLSSFFSFVNFELHEI
                     VKEGIRLALDVLNKITSDQPDRLIKDFKYHDCFGSYNVQIFSKGLPVDILETMIAPDR
                     LLSNLSGSRQIMKNASRYLKGFALSNRVYMKYGWRLADVDTSDYAKVMSDREMHARDD
                     LKAFCSVFFKECFASGKYEYEAYLSERQHAIRNGY"
     gene            complement(11441..12319)
                     /locus_tag="QBF46_001907"
     CDS             complement(11441..12319)
                     /locus_tag="QBF46_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674095.1"
                     /translation="MKLATDLVNAVLIKNGIVPTSINELPVTSLVKPLTQVHLSLYLK
                     TTHLSQFVPDYVESLKDCPVSYDIGLELQQCINSAELLCEDMESLEFISVWIELYTCN
                     SKQQRFEKSNFSFRTEDIELAKMLSCSHEILISQAACFWLLYFEDFMKYVRNRLCALL
                     RQVLILIAGLRNVYLQRNEDQLIKRVKHGNEMLEVTLKAVALCSIERYLKSYASLRTE
                     AAASGFIKCIQSYISTGWRHGYIVCQYSKNGRSLGKSLPLFIKETSEHNIQKRVNRLI
                     SSTISGRNKDLGLYDF"
     gene            complement(12397..12921)
                     /locus_tag="QBF46_001908"
     CDS             complement(12397..12921)
                     /locus_tag="QBF46_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674096.1"
                     /translation="MNIENQDLFNTFAAVISSHIVEQPSSCYYLHDNEIDFTILKHSI
                     IDKDKNLLYVIRPSGTCLLRCDKYFFPNYYLTSRGDYKAFKYVHFNLATREAEEITWQ
                     QAFEILSKPGRPPLRGSLGKFDYLKLVIDDLRARGYADFLPAYNLDGLRHFAVKDERP
                     SLVSYIDNVMALCA"
     gene            complement(13196..14428)
                     /locus_tag="QBF46_001909"
     CDS             complement(13196..14428)
                     /locus_tag="QBF46_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDQ6674097.1"
                     /translation="MTYASQAVKRTPHEVTDHFIKMVHARIAEVSGWRYVFDRIPAFK
                     DACDKAPGQVPCPFSGVGKSKFRFRKKDLFTGCAIHNDFPVNAFCDGIDVLAEYYKLS
                     KTQTCKKILTDFFGMDLYAPLTDADLESERRYKSTVRATETLDSDEVEKRGRKLEVIY
                     HYTGEIKPESPVWVYLRNRGLNRVLSNLPKDLGLNKRLYYMDKSLEKPTIYPGMIAIY
                     RDTRGRPLTIHRTFVELNGDKAHVENPKLMMKPPADMTGGSIQLYDPHFNPGTRTWTL
                     GVAEGIENALSVTEATSTPCWAASSAWCLENVEVPDSLLPPPGVKVIQFYIWADKDLV
                     NTKGTSPGMESAKRLQERMKEFFAKRYPTSELTIKVFEPDFDIPVGKKGVDWNDVLKL
                     TGPDGFPVKWAPECLAQL"
     gene            complement(14543..14764)
                     /locus_tag="QBF46_001910"
     CDS             complement(14543..14764)
                     /locus_tag="QBF46_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674098.1"
                     /translation="MTQIKTYRVEYEKVGTMHRVRIFGRMGEIVKSELPEERILRDVS
                     IPEGNGEMATSMVDGFIQRLENIGFKTEA"
     gene            complement(14836..16848)
                     /locus_tag="QBF46_001911"
     CDS             complement(14836..16848)
                     /locus_tag="QBF46_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ6674099.1"
                     /translation="MKFDLKSSPRHIQRLTNIASVISGINGIYVIIDNKVSTPYFNTQ
                     NNVCVLPNGDYSDERFVKLIEGFICHEAGHGRYTEHEVYREAFVGELINADGFISIDD
                     DLKADFQNLKQKQKAYARACRLKGLINLFDDVQMEEKTGIDYQEAKKRLAVTYALMVE
                     AGRMTVDISSTPQNPVQFIEMYLLNTLRVNVLQQEGHKETLDPFFDYAKKILAPVTSE
                     VDEIIHQALSCKSTQNCDSLARKTLALLERLRDEAKEKQQEEEQSKDPHDDTDESSGS
                     ENEPDTEPNGDSQGEGDGDKEGQGDDSGDGKAPAEGNGLPDQSGGDEQNAENSNGEPD
                     GESEGDESATPGSDDADSTPSLNDSSNGNSSDGESNFSPEQWDMLAKLLDDFLNSDEE
                     SEDFHEVLAKEISVIAASVSDEVKAEFGASEWDVPDLNIDLNVYNEALNISQTLGADL
                     SVLQQVKMRGQFKTRDRGLSFDINRLIQSPMGVRDVFRSQSESKNRGHVGLVIVRDIS
                     GSMSLEHRYIHAIKTDLALTLAIEAISKMHVANVIYPFVDKDFEVIKTFDENAEEKLS
                     KFSLGCKGNNTPTGSALNAALELLLESQFDRKIVFLITDGYPTESAYTINDVFSVAES
                     NGIEIAGVGIKTDVLMGFNEGTFVNVDDISLLPNEVSKLVHQILSN"
     gene            complement(16926..18053)
                     /locus_tag="QBF46_001912"
     CDS             complement(16926..18053)
                     /locus_tag="QBF46_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HDQ6674100.1"
                     /translation="MTFNTSKLNKVSAKEISENFIKAYPDLQEGAVITKIEISGCQGS
                     RRTDKIELSYGGDDITDQKSVSKSEVRWIDIKALSFKSTITSRISTLCSRLCIRYSNM
                     WILPVERMEEFLEEAQEIEREFQAGIQNVVDNYDTHIQAEKDRSPLMSDLIDQLKLTK
                     DDFVKSFRFNLAHFIPFSPISVEGDETQDFYQEQLITDLADEAMKVYTKISKNDNLRS
                     STIDRLKQMQDKIISFMFIHKEAVVLAEAIKHIMNNLPKGAISSPRDVAVLKQWFYFM
                     SDASMLKRIISGEQKVTDWLDSITRSFNIDSQVTAANSLNTAEDDVFSSANVFREDPV
                     EDLENKAVNAPEPAVGPDEDVSIQASGSNRAFDCELELTGW"
     gene            complement(18124..18393)
                     /locus_tag="QBF46_001913"
     CDS             complement(18124..18393)
                     /locus_tag="QBF46_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674101.1"
                     /translation="MTDFAHLTFYGFLSANGTETFVIAQDGNQYIGLKQHNNDYSFIC
                     YEVCNKDMEAMIKGLESKGFMYQGSIQSADVENEFPGILLTTLEK"
     gene            complement(18432..19484)
                     /locus_tag="QBF46_001914"
     CDS             complement(18432..19484)
                     /locus_tag="QBF46_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6674102.1"
                     /translation="MTNAATVLKSDNSVQEEIIGNLLPANVISSKMFANKEQYYLVKA
                     KDLFGIPEMDEDMIVPVFKKFSPYRANLNKDYIPNSRILEQVVKLLVSNDIDLSVCLK
                     GESGSGKTEMAMYISHMLNWPLTIKQINSNIRADELEGERSLINGNTSFVPSELVTAF
                     QEGHLILLDEVDKIDPDTAAKLHMPIERKPWSLAANGGEVVKANKFTRFMGTSNTNMS
                     GGSRKFVSSQRQDAAFIKRFLIVEMERPSEVALTNVLLKRYNNLPMLVIEKFVSVAVA
                     VNNTGTDDSVMDIRQLVAWVGTSMTLKTMSLLDTFKIAFASALPAHATGMVLEAIDLI
                     LGDDKNRTMEYYTAKK"
     gene            complement(19544..20530)
                     /locus_tag="QBF46_001915"
     CDS             complement(19544..20530)
                     /locus_tag="QBF46_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674103.1"
                     /translation="MKQKIRPTAFCSKQMDFFSIFDFEEEEKEIITIVESPLDYINDA
                     IAAQQSIQSEDKHSGPELEPSVSEDVALPGNCPKLLKNQILHDISFAAETDYLFTLEE
                     YSAELGGLVFDWAPEDVYQLYVVAMEESLENVRHLVITKSLYSTDEFGNITVNPLLEA
                     ETRWYMSKSFELTCATHGIDAIEFRSELKKSLYEYTHSYGGENAELARYHQDKEVILH
                     DCKEEMGWDIFFEQDYLLQENKLAMKWTDRDIMDVYSKVFKSTINLFEKLVVNNKLTL
                     RDTFGWVIVNPTFERQFEWIESEVFEIVGTHLGYNVAAIRTQMATACRMTFH"
     gene            complement(20591..20968)
                     /locus_tag="QBF46_001916"
     CDS             complement(20591..20968)
                     /locus_tag="QBF46_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674104.1"
                     /translation="MSYLEYVNEVKVSGIVISAMEKEMTSGNVGLLIKLKNRMKTEIN
                     GEITEREFSIQIKVSPEMYSGCFTGINQGDELMVSGYLVVDTITLEGREHPLDYMRVV
                     ATSKLAHIPKPPKGFGQSSFRQI"
     gene            complement(21027..21902)
                     /locus_tag="QBF46_001917"
     CDS             complement(21027..21902)
                     /locus_tag="QBF46_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000045554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674105.1"
                     /translation="MSKSKARSKALLIAFADLIPDMDKVVNKKLLDSLNVYSGHDNDL
                     IVIMNEDGPTIIELNSLKSVSMLAQKLSAFSTYYHVEMQQILVNPIDFEKAYTLLKEA
                     PAIPMFKTLADLDKFLNEEFEKYGLNTFLDVDNLDYSLAKSRELKNDQLVAWVSEIIE
                     KREKLALRNRFNEVTKAHYETVDAMYAAVRPLMKELGFPDELMLHTFSELSVFDSKGW
                     DYAIKSKIEFLTKREEQCLDYQMKADKRQATVDELLAQINNAKTVKAPRSFGQLFGFS
                     VIAMMTFMFIVNKFI"
     gene            complement(22300..24084)
                     /gene="trhI"
                     /locus_tag="QBF46_001918"
     CDS             complement(22300..24084)
                     /gene="trhI"
                     /locus_tag="QBF46_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer helicase TrhI"
                     /protein_id="HDQ6674106.1"
                     /translation="MGKPTDEQRVIIENANANNMVIAAPGSGKSFTMIEAVISILRQF
                     PYAKVGMVTFTRAATNSLAEKLKRRLSKKDQDRVLVNTFHGFIRMQLDMVNWKGKMLI
                     SSAQRSVIHRALKESGAPFRYPDAEFAIDAIGREMDTDIISVRHTRQQIHLFNTYQAI
                     CQKDHVADLNALSRFVVGQMYSGKMQPLNLTHLVVDEVQDTDSIQYAWISLHTRAGVN
                     TSIVGDDDQAIYSFRASGGVKIFQQFEKQFRPNIFYLNTCFRCEPEILKVAGALIEKN
                     VYRYAKDLRSAKGGGGKVHFRSYVDMDEQIQGILNLINQDPIGWAILSRGNAHLDQLE
                     SLIEQPVLRYGGKSFWDEKETSDVLHLMAFFRHSNDVRLMKRVLALFGENEEVLDQTA
                     LSMKGRKVTFGELNIPNESSLETRTLHSNFTRFTQETREKVEIEKRFANLIKWMELSS
                     IKMRTQKGSPSLSRIALDTCKQWAEKTGWQNMINRAAAMCLGPKKKDEEYTPDKVVLS
                     TLHGSKGLEWKNVIIMSCNADQIPSKRSVGQEAIEEERRLLFVGFTRAEQQLHVMWYG
                     DPSFFLSECAEDKLKEAAKSRTESPLTE"
     gene            24266..25138
                     /locus_tag="QBF46_001919"
     CDS             24266..25138
                     /locus_tag="QBF46_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6674107.1"
                     /translation="MKNKRFVSLVSALVASASLVGCAGNAGQSPNSAQHSDTHGLYWH
                     ANAKDSVAKNAYSLAGFDVSFNEETVQPGGDADNFLSKLLISGSMGYVTGGLSGLSIM
                     SLGSLYSSSDAEYIQVNQYVVFVPNPKKLPYNDESLVRAGAAYVYNHTKESQVMLGFN
                     PSKQSAALASCKIDRAVINKWSTCELTSKPAPDVMPTNSMYSFQAIRPATGTEIPQLN
                     LPAGEYSVIRYVFIPFKGNESSNNFSGIIFRSDSPKFTMPGGAAASINGKDYYLFSGE
                     YGQKGFPEKTLKSK"
     gene            complement(25166..28342)
                     /gene="trhN"
                     /locus_tag="QBF46_001920"
     CDS             complement(25166..28342)
                     /gene="trhN"
                     /locus_tag="QBF46_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhN"
                     /protein_id="HDQ6674108.1"
                     /translation="MKHRKIISSAIIASMIANTMSWAFYVSLINIALTPKIFAADAIF
                     DQLENNFNLANPNANRNATTSAQDIVEKYKNADSGENLSGKISEKYVGKAESTNLNVG
                     KYGTPNSNESVMSNAVSDGKSIGKAVQLPSMSGGTINSNYTKEGAKLLSRDSSGNIGI
                     SNNPNTTAGTKTSTGELFSSEQKHSDVQFNAGGRYGDENGFINDIKNRKSQLFEAQSY
                     DGVAYRTLVNANKENPASTIKPNDPMFNAGRNEIGNAVAGTGNWLQNCNTETSKQTIT
                     THYPDYKEFYCNSPKKDNFNSCTITRDFSVPVYISGGNGDMSMCGDNCVRVWFGRRDD
                     NYWNDGVYDNSLTLKFHPDAKLATAKIINAEWDDHMRVTLDGTQIFAHIDGAYRSSNY
                     PSPQGQWELKKSWKLDKVYDITEQVKTSVYQEQDREVTMASRVWVGGGGEGYFEVEMT
                     FENMKLEDKHIQEPAGCFDAVQTPNSFCRFDRFVNMDVGTKRLPESVLKMATPLYKGD
                     TGYLTWKTNLEGYFCDPLAKEKLCSYDAKGNIMKDPTGKDLCYNYDDIKNMPDACSTY
                     KNDAACVLDNQTCAEGWFDEGTNTCYMYEQKYTCDRGKDVVREVESQTNSCVGMIPCS
                     GGTCETGPKEENKDFGKVAAYSNMVQYMQGEAKCEDPNDPNSCSVFEGKAEWCGRSVG
                     FVNGLAKTDCCEKPQGAAGSLEAIMLAGSMIRNTNWTRVNAQLVNWTGGESGTWASMA
                     NSVGEWTASAGKTVGQMWNNVTSSITSVYENVAGNLGRTVGSSAAGESGQLAKETMSS
                     FGLGKLKQMAMEKAYDLLPDTVRDFVFKNVATTGGEIVFSAAVQNFMLALNVIGWIYT
                     AYQVTKMLLEMLVACDQKEMEASIHKNQKSCFTLDTNRCVKYLNLGFTKKCVKKATDM
                     CCYNSMLSRVIMQQAYPQLGIDPVTSNCVGLSIGQIQKLDFDKIDLTEWINDAVQVGE
                     VPDQYATFSESSITANLPFKNENYQLPSERTKDAMGGEENMIKARQENAQAIKEENVD
                     CSYLPRPAICEVGSTYVDPITGKEIPKY"
     gene            complement(28365..29417)
                     /gene="trhU"
                     /locus_tag="QBF46_001921"
     CDS             complement(28365..29417)
                     /gene="trhU"
                     /locus_tag="QBF46_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhU"
                     /protein_id="HDQ6674109.1"
                     /translation="MKKLKKLLLEILIAVMLSLSLPAMTMAADTGVPGAMCQSAGVWQ
                     GLIKNICWSCIFPMRIMGIGAAPEGAAPSRPGCYCTDQNGIPEIGWQLSFFQPVKIVE
                     VVKSPWCSPFLEGTMLQKSQFDIGKSNTNQPMTATEAGFYDVHLWEFPIMTMLKLLVI
                     GECTAEPYVDASLTYISEVDPMWESDLLTLVLNPEAVVFANPIASMVCAADCVAVTAG
                     KDNLAAYFCAGCDGNLYPLTGHIYANDDAVRTSSLITQRLLTKLHRQGMLMRTMGADA
                     MCEKTWEYFTPRSQYRLSMLYPTPEANGPDCCHRLGDSVHDWSTLKGGRKKIGIDNYV
                     YMLWRYNDCCVRYIPN"
     gene            complement(29562..31070)
                     /gene="trhW"
                     /locus_tag="QBF46_001922"
     CDS             complement(29562..31070)
                     /gene="trhW"
                     /locus_tag="QBF46_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhW"
                     /protein_id="HDQ6674110.1"
                     /translation="MRSFELKTTIKPLASCLAFYALISTAVASPQYQQVADDIARQAQ
                     VLGAQIPVPHASEGPLPSGSLDSPDTKKYIRQAEAMKKNGDLSQQTNRGYVPGMNADS
                     VQAVIDHTQAIRAQSNNSEAVNDIIRRRDEIQGNSQLSESALKSVENKPEVMRSQSSN
                     IEKMFGSSGITAADFERKIDSSREEVLSTENGITIFASFSLPDYVLEDLLRTASEHKA
                     RVVFNGLKKGTTRLPETQAAINQLVVKGKFDSPLITIDPDAFNQYQITQVPTIISREQ
                     SRFAKMVGSFNVDFFQRELARKPDQDLFPIAGTTYPVEEKSIIKELEERAQKYDWDGA
                     KKRAVADTWKNQYMVNLPPAQEHKEWLIDPTIRVTQDVKDKQGRVIASAGELINPLAR
                     FPQNLTMIIFDPMNPGQLEWAEKQYRQRLGSGQVMPMFTRIKQENGWDHLNDLREKFN
                     GKVFKVNEQIIARFQIKNTPALISTEKEKFRITQFSEAEIRGIGTKIAAEEN"
     gene            complement(31057..31569)
                     /gene="trhF"
                     /locus_tag="QBF46_001923"
     CDS             complement(31057..31569)
                     /gene="trhF"
                     /locus_tag="QBF46_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein TrhF"
                     /protein_id="HDQ6674111.1"
                     /translation="MREYIPKKRGLLSFDWYVPINLGILILVMLIFFNRFTFGHGLLN
                     GCLPADFYMIDLKDKSVSSGNIIAFHMPKSVRFIRENEKVIKIVAGVGGDRLRVTLDG
                     VYNGNKFYKANARRISVKYGIPAETIERELTIPEGEVFLIGQTDHSWDSRFWGPVKLT
                     SVIGKTYAIF"
     gene            complement(31843..36141)
                     /gene="rsp"
                     /locus_tag="QBF46_001924"
     CDS             complement(31843..36141)
                     /gene="rsp"
                     /locus_tag="QBF46_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncHI-type conjugal transfer protein RSP"
                     /protein_id="HDQ6674112.1"
                     /translation="MRKSGLCLTLLFSLIASIKSVQAEAIMISGKLQADMPAVTFNPG
                     PGDFVAFVNNNTITASGTGNACNVTVDDRATSSVDNLVCFFEWLPNTSGFTANGFNLT
                     GIPNAAGELKLPYKISYFSGSERQKVEVVNGEYTVNALVPVKPTITGLKSSMNGVVQD
                     GYTLKSYLKDEAIRNVTVSVEPRNYVQYISIGSGSACEVAIGGNGCTIDVGSIKVSDS
                     DLLMGSREITVTANSKNNYFAPPEAQKLTLTWDYRPPVVDHTLWNFTDEAKTISIGGQ
                     EIYTGARTVAVAVKVPQEETEGEWWLPTAMTLTMNPDGVFKPTTKVTLEDGTVIDFKQ
                     SWATPLRRTLQPVSGPQKVGDEYLYIFDLTDLTNGSYAASFTVENNSKNTSTYNEPES
                     KLMLSDNPTLMVLKDGAALAKRAPVYFLNEIIVAAFQGQAGVADIKAVTIDNKLVELT
                     PTNHKGIYYLPVGDDLEVNADHEITVIAENLYGKIVTFNTTFTYQPTGFTLKNFEKDI
                     TLYSRVRQYTDLMSQTAGDKCTLFTTEENALAYLEWYGDKSDITACYPQWNNVPDGLE
                     FYFKGRTPGLTGFFNKTGDNLLDYQVYMINGKGSKAVSSRNRHVLITQLPYNPVLTYK
                     KNKVLTGINDNTALAYTTGGEAARLLAKVVPADVTVYVSQNGGESVKTTFKNRSSNND
                     ATTFVQRLKVASAPLWTKNVFDIAVEYSKEPTMRTVDTLNVYTVPDFNIRAAMEIEDK
                     KTATTMELPLKVSVGRYNNSTRKSSFSRTTMGDWDVTIYAQKSVYGKDPETGRYKTTY
                     ERTPLTEALPVNDAGVVETKIKIENMDLGNVRLVGIAKVRSPFADFELTRETSAVSIR
                     VYKGEELEGNLSKSLIIGRIPLSTLVNFKSASTANSDALAPTEWQESSDNGQTWTMLQ
                     DMTGKRSISIRKTEVGKWLYRAKMTNKFTSKESYSDVLTVVTYKQPKLSIDVAEILEG
                     DDLPVTLLDNDEPIPQGTAEVMWSEDKVNWVQGDTTYNVSAADTLPPTIYARMRYLDS
                     DELAEESSWKETSARLTVAKPKRLSVSVSGISSVEVGSKVTLDGKYTNPNSRFKNGND
                     VIEEWTAPDGQKIRGANLSITLTEQMLDKQGYAAFEYSAWLADSKDSTISTRKVSVKS
                     WVYKFPEMKVSTKLKYTMAPSTLHVALSGIKDGGYPGVTYSREWIYDKDNISVLKDDG
                     DTKEFAIAKPGKYTLVIVFRDNRNNEQRIENTFVVDEQTPMTVEMTPKFSNKFMRAPL
                     DVTLRSNIKLAHSADSIDTVTYKVNGEVVPGSKNYWAQLISGLTEKKYEVTLDVVSKM
                     GQRGTASVDFEVVKNAAPQCALSYTESNMSWSFTNKCIDTDGKLVRYEWYINDELRNV
                     FGSTATLSKNLNRGKQDIRVVAYDDSGDSATQRTTVYGPDEGASKSVDSVETSQ"
BASE COUNT         9905 a         8610 c         7169 g        10541 t
ORIGIN      
        1 tcgtccaagc tcgccgcgtt taaataaaag gaaatgaagg gaacaggtaa aaatattaga
       61 tgtttaattc tcatacgagt taccaccatg gagcggaaac aggattttga atgacgtgat
      121 gactttacta attaagctta tttagaggta ttaatagaat attaaaaaat tttaatttta
      181 ctgctgacgt tttcataaat ctcctttcct gaaaagtcgg cgataaattg gttaccaata
      241 tcttcatatt actgacggac cagtgtccat acgcttgcgg gtactttatc gtagagtttc
      301 ttcattgtca gttcagcttt tcgatggtca tatgccccgg aaatttccag tgatgctata
      361 cctgcatttt tttcacagag tcgttcgaat actttttcca gtgtgtcgaa agaagagcac
      421 ttacgaaatt tcattaaata cgcctgctta gtttcggaca tggtcaacct taatttattt
      481 cccgcgaaag tacggtttcc caatcttatg tcgttttgtc cctagaatct aatttttttc
      541 acaaatattt gatgtttatc tccggatcgt tgttgtagtc gctctctctt ttttagttgc
      601 tgtgaacctg ttcataagtg gatacctcag tgctaaaata gctgtaaata aaaacagtgt
      661 attcaggtga ccaccatgaa cagcaagcca gtaaccagac agtttgaaga ctcagacctc
      721 catcaggaac tggtcacgtt tgaggtgccc aataatgacc ttaaggagct cattttttac
      781 ttttctcata tgaaatacaa cacggctaaa acttaccttc aatggctgcg ttcctggaac
      841 gagtggtacc aagctaatgc aggaaaggag ggaaacgaag cctggcctgc cagttctctc
      901 ccggtgacag aacccccgtt gttggcctat ctggactatc ttcagggctc gctgtcacac
      961 agcagcatta aaggttgtct gcacgcgctg aacagtatcc ataggaaagc actcgatcgc
     1021 cctgggatca tcacatccaa ggtcaagagt attcttgcaa gcctcgaaca agctgaagcc
     1081 cgagagcaaa aagtgactcg tcaggcaacg ccattcctag tctccgacct gaaagctttg
     1141 attaaagccc acggtacgac acagtcggtg cgcaaactcc gagacctttg cattatctgg
     1201 acaggttttg aaacactgtt gcgctcagca gaacttcggc gcattcggat gcaggacctt
     1261 gtgctgaatg aacagacagg cagttttacc ctgacggtgt accggacaaa atccactgtc
     1321 agtaccctgc ttacctatca tctgacccct catctcactg ccaccctaat caggctgatg
     1381 gatatggtca aaagggacca gcaaagccat ccgaaggatt atctcttcca ggcagttaac
     1441 tatcaggaca gcggatatat gccccctgga tggggtctcc ggagcaaagg gaatgagatc
     1501 aatacgctgc tgaaaaatca taatatgcct taccgaccta cccgtccccc gatcgggaaa
     1561 aatggaaagc ccattattgt cgatgatgag ggtatgctca gtaaaaacac attgctccgg
     1621 gcctttgaag cgttttggga tgaacttcat ccgcaggaag cgggcacccg atgctggact
     1681 ggtcacagcg ttagggttgg cggcgccatc gaacttgcta atgccgggta cacgcacttg
     1741 cagatcatgg agatgggtaa ctggagcaat ccagaaatgg tttcccgcta tatccgtaac
     1801 attgacgcag gtaagaaagc gatgacgaag ttcatgcgtg aggcgctgga tgagtagcgc
     1861 ccctgcgggc gctaatgagg attacttctt tcgaaatgga ttaaagcggc caagatcatg
     1921 aggggcgtct ttcggcggca tcatcccggt gcgtttaaat ttataaaccc cgtttagctt
     1981 catcgtctgc acgcccagat ttaacagtgc caccagcttc agggtataca gaaacgtgaa
     2041 tgccgggacc atctgaataa aaataaatag caaacccgca cacatcacac gcagccaggc
     2101 tgcatactcc atgctcagcg gccgcttaaa gtaatggatt tccagcaatc cgaccgtctt
     2161 gttactgatc cacaccgctg ccagtgagag taacaacacg atgcacacca taatgcccag
     2221 ttccatgatc ctcactcccc gtcgatttga tattttgagt ttctgattta cccgctttgg
     2281 ttttgttttt cgccttcttt ttgggtttga tatggtggaa caggaaacaa tccaccagca
     2341 cccaaccatt agcatatcgg gccagcagta atttctggcc cggataatgt gtgtggaaaa
     2401 gcattttcac atcaacggta ttcatgatct tctgcttgcg cttcagttcc tccccaggca
     2461 tctcaaattc ctgaataaac ggtgatgaca accgctggcg aagcattcga attgcactag
     2521 cgttttcatc gtgttttgcc agcctcccga atcgcacaag cgccttatga gttacctgaa
     2581 caggccccag atgggaatta accagttcat actgccagta gtatcgcaga tccactttta
     2641 cccagctgat gctgtcttcc agtgcggctt tcatgatggt tcccgacatc gggaccatgg
     2701 tcagcttggc cagacgcatc tgaatgtaat cagccagggg cctaagggac tcaggtacat
     2761 tgctgttcag gagaaggtgc tgaagccccg gtgtcgacgt ctgccagctc aggttgcggc
     2821 cgtcaggcaa aaattcaaag ttctgatccg ctggcgccga ttttgcgttg ttctctttcc
     2881 gcatcagaaa ataatgccca cacagtaact cggcccgtac ctggtcctct atcgggtcat
     2941 ggcgggcctt actgagatca accgtcaaca caacacggtt gatggcatcc tcatggtatt
     3001 ttttcaccca gacacagaca cgataaagtc tctcatcaag tttctggctg gtcgtggaaa
     3061 tccattgcat gcttttctcc tcagaccttt ctcataatga aaatgccaag atctgcaccg
     3121 ggtacaagtt ccgtgtagct atggttgctc cttcgaccat tggacagtgc ttcatcctgc
     3181 gctggcaccc agtcctgaag tcttttaatt gttggctttt gtccgacgcc aaaaatgggt
     3241 aatgaggctt tcaccacctt gtaatcagtc tcggcatatt tgctacttcc acgctccttg
     3301 ccttcaggcc ggtatgtgaa caaatgcgct atcggaaccg ggaaggtatt ggttcgacaa
     3361 aggcgatacc cttcgccgtc tctcagtact tttctttcca gtaatgcctt cgctctatcg
     3421 atgttcgcca ccactgtcag gtcagttgtc acgggcgatt tgaccgctac taaacgacgc
     3481 ttatccagca tcgcgatgag atcacttgcg tttgttttta cctggtttcg gtttagccgc
     3541 caggcaaaag taactttttt gagctgttct tccacaaaca gaattttgcg agtggaatgc
     3601 gcagtcacca gaccgggaag cttacggtga acttccttaa accggatgtg tttctccgaa
     3661 ttcacccttt tcattgcaga ggcaaagtca cttttggcat catttatatc gcaaataatt
     3721 ccgctcagct gttcggcacc ctcaggaaca ataatcaaac ccgggtattt ggttgtcact
     3781 ttcgtcgaat tcctgctgag tacatcaatg tcatcgatac agaaatcctg aataacacgg
     3841 ataatttcat ccacggaggc atctgaaata gtactcacct cgatggggtt cacctctttc
     3901 aaccgctctt cgtgtcgtgt gacggtcccc acagtcatca gctcggccat caccggctga
     3961 tggtcattca gaaaggatct gagcttttca attcgctcca gaatccggtc acggtaaaag
     4021 agtaaatctt cactggccat tcatcactcc ggtttcaata aagcagcgca acaccgttac
     4081 gtcttcaacg acttctgaag agtcgatatg cagccggtag ttgtactttt acagcacctt
     4141 ccgcaattcc ccgggattgc cggttgtata aagtggcaga ggttcaacct gctgaccgtc
     4201 atgccgaaaa caacgattaa ggtcattcaa aaggccaaaa ctgaccggct ggcgttgcag
     4261 attgatgcgc tgccagtcct gctttacacc aaacttcacc caccaaccca gaccaatcag
     4321 ttcattacgt atgttctgca cattgcccaa atccgatact gaaacatccg ggattacacc
     4381 cttcccttcc accagcaaat gcatcacgct ctaaaaatca ggctgacggc tcttccttgc
     4441 gtgcttcaga attgtacgaa tcgaacgcaa gctgtggcgg gaatgcaaat tacgacgttc
     4501 agccgaacta agccatctct ggataagctc atttgaatat gacaccggca tttcaccggg
     4561 gcctgacctt tcgatttcac gcacatacag caggcaggaa gtagcggcac tcactactgc
     4621 gtcagaattt tcgttcatgt tcacgtccgt taatatcaga aatctgctta tccggtgtca
     4681 ggaacaaagg ctgatagtac aggcgccacg gatagtgtac cgtcaaacca tccgttccaa
     4741 tccggttcaa ccggtagcgt ccattaattc tgtcaacaga ccaggtgcca ggaactacag
     4801 aacgactcca gcggaaaacc ggcagatctc cgagctgaag tgtattcacg agccctttat
     4861 ccatttcggt cacgcgtacc ttcaacaaga agcccagcca gggtaaagac cagatacaca
     4921 gccaaacggg ctggaggaca actttgttta acaaatgacc tgtcatgaac atcgctgata
     4981 aaaacataaa actgatgata atccagaaca ccatccatcc taaccaggct cttgtctcac
     5041 gaagtttcca ttcaagaagg taaagtcgct ttttcatcca gagcccccgg aactcagtga
     5101 atgactgatg tctgctgcat caaaatagtg gtgcgtggca ggatctcgtt tcgccacaac
     5161 tcttgagccg tcgtccatcc atgtagaatt ccgttgccac agtgtgaatt gaacgccatg
     5221 acctcatcag ttagctgata gaccagcctg tctggcattt caatgtactc cggaagcgaa
     5281 agcaggacgc cgtgtatcac gtcgccggta agcctcgaca acgtctcttc gtcgtccaga
     5341 ttctttgtgg gcatcgtcga aagcgggata gagaaacaac agagatccat tcccaccgcg
     5401 tcaaaaccaa catcaaaacc ttcgtcattg agcacaaatc ggaagatagc ctcaggcgcc
     5461 tgttctagac ccggcagcga gccctccggc acttctgtgc cggtaaaaaa cttatcagtc
     5521 cagttactga atggactggt gaacacccaa caaagcagag gcaacgcaat atccgtttta
     5581 agtactcgtt tcgtgccgga cgttcctgaa gagtgaattt tgcttttcat tatttacctg
     5641 tagagtgctt tacttacagc tatataaaca cattccggaa tttccccaat aggttggaaa
     5701 acactaaaat tcacagatac tggttccctt ctgttttagc cggtgctcaa gatagatact
     5761 tgccaggtcg agtggctgat tttcttcgag acaggcctga acaaggtctt cagtggatac
     5821 ggataccagc cctcgggttt caagaatttc tttcgccgtc gtcaatgctc gtgaggcgct
     5881 tcgacttagc tgaattttca cctgagaact gttttcacac agggcatcag ctggtacggt
     5941 ctgtgaatct gtattgctca taggggtatc actccatatc agggatcgaa tgaaaattaa
     6001 ctgcaataga cttctctggt tgaccgtttt ctacagtgct tagccaggtt cgagccggaa
     6061 gttgttgctc acaaagaagc agatagccct cgccatttcg gtgcttttgt cccagagcat
     6121 gcaggagatc cccagaatat ggacccagag ctgttcgggt ataaaacaac gtcccagaat
     6181 gtatcacttt cgacaatttg ctaagcaaaa cggtcttctc gtcttcattt tcgggtaaga
     6241 caaggattat cctgaatccc ttggctacat gtgattggag atctgccacc agcgtccgaa
     6301 acctgaacgc ataaataacc aatctgaatc gggtcattaa cagcgttgtc gcggcaaaaa
     6361 taacgacaac tgagccgaat ttagaacggt agaaccacca gttgagggca ccggccacta
     6421 tcaaaacgac cagcagcaca atgcttccgt aaaaaaatgc agccggaaaa cgggttcttt
     6481 ttccttcgag gaacgcagat gccagtatta cgcctgtgat gcaatacaca acccagtcaa
     6541 agaacgattc agtcatcatt aggtgatagt gtctcgtgtt gtattaaatc ggacgcctgc
     6601 cgttataaag tcctcagcaa tcgtgtccca tgcagccatc aacttcgcta aaattgtcac
     6661 aactctcggt tgtaacgtaa cgttccccac tagctaaacg gcgctgtaaa tcgatcttta
     6721 cttcagccgc cggtttgaga aacagatatt ccagttgtga gtcagttcct gaagaaagca
     6781 gtagctcaat actgaaatca atatgccggt atgtgaaatg gctattgtca ttagagaaga
     6841 ccgaattcac agggttattg ctcaggattg tagtggcagg catagtgact cctttgtttt
     6901 aaacagtata caaatctcta acgatccatt tttcagataa gagacctatt ttcgcgaaac
     6961 cgaaacaaaa gaagtaaaaa gtaatttgga ggctccagca ttcggactgt gtgctgccgg
     7021 aaacgtcagc aaggggcaaa acattcgagt aaaagaagca gagaaggaaa cagctggaag
     7081 gatgtaaatt aggctaggtt tggttgaaga aggtaatgga aggcttacat gtaacggtgc
     7141 caagagtaca gtaccgtaaa acatataggg cagtaagaac gttgtaaaat tgtaaaatca
     7201 atgcaagttt tgacgaaagc aaaaaaaaag gcacggataa ccgtgccttc ttagaagtat
     7261 aaaaacctga aaatatataa ttcaaccttc aggactcatg ccgcatcaaa gattttatcc
     7321 tctgatgctg ggatgaattc acgtaagtta gcgaacacct cagaaattaa ttcctgacgg
     7381 tcatggattt taatccgacc accagctaat tctgatacat tcaccccttg gtaaagttca
     7441 gtaacaactt caccatctgt atcaaggact ctaacattag caaccatagg aaagtaagat
     7501 tttgaagcat atccttcata aatcccaata attgattcat tattacggcg taaaaccacc
     7561 ttgccagacg gcagcgttgt ttcatccata taacaatcgg ttaattgctc ttcgaaatcg
     7621 tcctctttaa gtgaaatatg caaactaagt tcgtcggtaa tacgacgcgt atatgaatac
     7681 tcactaaatg tataaccggc catgtttata tcactgaacg tgcgataaac ctcataaagc
     7741 ccggatgtta tttcatctga tacatagttg ataagcccaa taaaatgcgt atcccagaat
     7801 ttttccagtg caaatcgggc gattaaaacc tgaccatcaa cttccatcag tcttttgtac
     7861 tcttcacgat catagccacc tggctgggcg ctgtccgcag agttactcag gatctcacta
     7921 cagacatcgt cgtattcaaa gcggctggtc gcgaaatgtt taccggaaat atccagatat
     7981 tgaccggaaa ccaggtcgaa aacctcaagg aagtaaacca gtcgcttctt gagtgcatcc
     8041 gcatccaggc cagccagttg ggtatcatta agagtatcca cataatgccc cgcagattct
     8101 cttaactcac tggctgatat actgcatgta aacgaaactt cgtcgctctg gcaatgagag
     8161 acactgataa acatctctgt ttcatcaaaa tcaataccaa tgcctgccag tactgcacga
     8221 acgtagttct ggttatcagt agaattagtc atcttcactt cctcaggatt attaaaggaa
     8281 gtgccccaca ggggagcgac ttccccagtg ggtggattaa aaatatgtac tactgcgcca
     8341 ttctttcctg caagttgatc agaataggta gcgcggtcat ttggtagaac aaagttactt
     8401 tttggaatga agaggtaaaa aacaagggtt aaacaacatc ccgtaactct tcgaccgctt
     8461 cttggaccaa cgcaactctg tcacaaacct gcactcggcc ggtaggtccc acggaacaga
     8521 aagtaccgtg cgatgattca gacaaaacac tcccgtcaca gccagtgaat cgaacggtca
     8581 tcattacagg aacatccttt ccggaactaa cgccagtata aagacgaatg attttttcat
     8641 catcgcgata cactcccgtc gtcccttcag actccttgaa accgatgtaa aggtctggca
     8701 tacaggccgg gtattcatct gtattcaaat caagatgcaa agtcaggtca tcttttacac
     8761 gacgctgata ttgatattga cctaaacggt taaatgagtt ctttacagat ttaattatct
     8821 ggagagaagt attcaaacac ttggagatac ttttctcgaa ctctaagatg aaatcaccaa
     8881 aatcagaatg gaaaaattgc ttaagtgcaa aacgggcaat gagaacttta ccctcaacac
     8941 tcatgcgatt ttgatagatt tcctttctta aaacaggatc tattgaatca tgttcagact
     9001 ccggtacaga ccccgccaat attaatgatg cgatatcgac aatatctata gtactgtgcg
     9061 cgttatgata accgcgaatt cttatgttct gaaaaccaga cttttcatat actttaagaa
     9121 agtatttgat tttagtccga aggacttcag tttcagaata cggcgcatta tgcttaatca
     9181 attctgatat atataaatcc agataaaata acaaatcatc aagcgccaca tttgctttaa
     9241 aagaaagttg ttcaaaatga ttttctttgt catatattgt aatatcctct tggcttatat
     9301 tcaggccaag ttgacattta agcattgcac taacaaagtt tttatcatca ctttcattaa
     9361 acatattcac ttcctcttta atgaagaaag gaagcaaccc ccacggggaa tacgcttccc
     9421 caatgggtaa aattaattac atagaaagca ggtcatctga atgctctgga gcatacgaat
     9481 taacttgctt aagcgagtta tttatagcct ttcgataaag cgatgaagag tgtgactgaa
     9541 ccattttata ctcagttacg agaagaggtg atgtaaagca acctaagttc gcattgaggg
     9601 ataaagatcc accgtcgcaa gcttctataa cagcgtaaat acgccgcttc atcaccctac
     9661 ccgacagata acgattaaca tggttcagga acttatccgc tcctgaaaca ctacacacat
     9721 tccagtcagc agaaattatc tggtctgcga ctgaaacggg tacaggttcg actactatct
     9781 gggcattgat ttgatggtca acactgaaaa agctggtgac tacttgtgga actgaactct
     9841 ccgcaatctt ttctgtaact gcgaaaatca gcggcaaagc cgttgataag cgcgagcgta
     9901 aaagttcatg cagatggttg ataaaattat gtcctttttc aaccatacca agtattagat
     9961 ccttgtctgc attgctggag cggtaatata catactccag tttttcacgg ttcacaccct
    10021 catttagaca gtccggctca acatccacaa gccgcaggtg agcagttgac actgaaacct
    10081 caaaccgaaa ggtttcaaga gtgaccggcc cagccttcaa caacgcagct cctgagcaat
    10141 attgtgctat agggtggtaa tccacgtcat gtccccataa aggctcacaa tcgcgcagat
    10201 atcgccttaa atgcggcatt aaagtccgga caggaatatc agccttaaca actagactct
    10261 gaatcacacc cgtttccaga ttgacacttt ggatcacggc accttcaaag cccattttca
    10321 ccagcatcgc ttcaattatt ttataaatcg ctttcatttt gtagccctcg gttaagaggg
    10381 aatcctcccc aagggaagaa ttccctcagg gattagataa gttgataata agtattaata
    10441 cccgttacga atcgcgtgtt gtctttcact caggtatgct tcgtattcat attttccgga
    10501 tgcgaaacac tctttaaaaa agacactgca aaacgcctta agatcatctc tggcatgcat
    10561 ttctctatct gacataactt tcgcatagtc agatgtgtca acatctgcta aacgccatcc
    10621 gtatttcatg taaacacgat tactgagggc aaagccttta agatagcgac ttgcattttt
    10681 cataatctga cgactacctg acaagttaga caacaaccta tcaggtgcaa tcatcgtttc
    10741 aaggatatca actggcagac ccttagaaaa tatttgtaca ttataactgc cgaagcaatc
    10801 atgatactta aagtctttta taagacggtc tggttgatca gatgtaatct tgtttaaaac
    10861 atcaagtgcc aaacgtatac cttccttcac tatttcatgt aattcgaaat tcacaaatga
    10921 aaagaaggaa ctcaggccat ccttgaacgt gagatagtct tcacaaaaac gtccttgtcg
    10981 ggtcagatgt agcaatcgtt ccttattata ttcacattca gtcacgtcgt aactatcacc
    11041 gcgtgtcaga gtgaatcctt cccttgagga aactggaaat ctaatatggt caattaaaaa
    11101 agaccgattc cgtttatttt taactacatc taactgacat ttaacacaat ccgcgatgtc
    11161 ctggtcttca aacccaggaa acgggatatc taaaaatgtt gaactgtatg cgggggcata
    11221 cgaataaaga aaatcaaatg aaacatcggc gtcacagcat attccagtaa atttaccgtc
    11281 ggtgccactc aagcagataa cattactgac cttcactgaa tgtttacgcg atatcagaaa
    11341 tgcattaatg attttctcaa taattttcat gtaatcctca cttaaataaa aaatgggatt
    11401 acacccatca gggatgtaat ccccggatgg gtaatgttaa tcagaaatcg taaagaccta
    11461 aatctttatt tcgccccgaa atcgtggaag atataaggcg attgacacgt ttctgtatat
    11521 tatgctcaga tgtctctttt ataaaaagtg gtaatgactt accaagagac cgtccatttt
    11581 ttgaatattg acagactata tagccatgtc tccaaccagt actgatataa gactgaatgc
    11641 atttgataaa cccagaagcc gccgcttctg ttcgtaaaga ggcatatgat ttcagatacc
    11701 tttctattga acatagtgca acagctttaa gagtcacctc cagcatttca ttcccgtgct
    11761 taactctctt aatcaactga tcttcattcc gttgcaggta tacatttcgc agtcctgcaa
    11821 tcaaaattaa aacctgtctg agtaatgcac agagcctatt acgaacatat ttcatgaaat
    11881 cttcaaaata caaaagccag aaacaagccg cctgacttat taagatttca tgcgagcaag
    11941 acagcatctt agccagctcg atatcttcag ttcgaaaaga aaagttactt ttctcaaacc
    12001 gctgttgctt tgaattacac gtatagagct ctatccatac agagataaac tcaagtgact
    12061 ccatgtcttc acagagcaat tcagcactgt tgatacactg ctgaagttca aggccgatat
    12121 catacgaaac cggacaatct ttcagagact caacgtagtc agggacgaac tgagataaat
    12181 gcgttgtttt cagatacagg ctgagatgca cttgtgtcaa tggctttacc agcgacgtta
    12241 caggcaactc attaatggaa gttggtacaa tgccattctt tatcagaacc gcattgacca
    12301 gatctgtagc taatttcata agacctccag attcaactct gagggccacc ccgatgggag
    12361 gtgccatcag ggatgtagta aaaatatgtg ttatctttat gcgcaaagtg ccatgacatt
    12421 gtcgatataa ctgacaagtg atggtcgctc atcctttact gcaaagtgac gaaggccatc
    12481 aaggttgtat gctggaagga aatcggcata ccctcgcgca cgaagatcat caataacaag
    12541 ttttaaataa tcaaatttac caagtgaacc tcttaatgga ggtcttcccg gcttggacaa
    12601 aatttcaaaa gcttgctgcc aggtgatttc ttccgcttct cgtgttgcaa gattaaaatg
    12661 cacatactta aatgctttat aatctccacg acttgtaaga tagtaattcg gaaagaagta
    12721 tttatcacaa cgcaacaaac aggttcctga aggacgaata acgtacaaca ggtttttgtc
    12781 tttatctatt atgctgtgtt ttaaaatcgt gaagtcaatt tcattatcat gaaggtaata
    12841 acacgaagat ggttgctcaa cgatatgaga tgaaatcaca gcagcgaacg tattaaataa
    12901 atcttgattt tcaatattca taacaatgct ctccgatatt taacgatgag catcacccta
    12961 acggtaatac tcccgtcagg ggttttaagt tttatggata taccatgtca tacaaccgac
    13021 cgtagccaga tgtatcgcac atttattaaa ctgagcaagc atctcagccg taaaaaaaac
    13081 ctgaataaca ggtgattttc acgaaggaaa tcggattatc tgtttaggat ttaacgtaaa
    13141 taaacactaa agagataacc ccccccggcc tgaaaaggcg ggggagttat cggatttaaa
    13201 gctgagcaag acattcagga gcccatttta cggggaatcc atccggtcct gttaatttta
    13261 gtacatcgtt ccagtcgact cctttcttcc ctacaggaat atcgaaatca ggttcaaaga
    13321 ctttaattgt cagctctgaa gtgggatatc gctttgcaaa aaactctttc atacgttcct
    13381 gaagtcgttt agctgactcc attcccgggg aagtgccttt tgtattcaca agatccttat
    13441 ccgcccagat gtaaaactgt attactttca caccaggggg aggcaggaga ctatccggca
    13501 cttcaacgtt ttcaaggcac caggcagagc tcgcggccca gcacggtgtt gaagtcgctt
    13561 cagtaactga cagcgcgttt tctatgcctt cagccacacc taacgtccag gtacgggtac
    13621 ctggattgaa gtgtggatcg tacagctgaa ttgagccgcc agtcatatcg gcaggcggtt
    13681 tcatcatcag tttcggattc tcaacatgtg ccttatcacc atttaactcg acgaacgttc
    13741 tgtggattgt cagaggacgc ccacgggtat cgcgataaat tgcgatcatt cccgggtaaa
    13801 tcgtgggttt ttccagtgac ttatccatgt agtaaagtcg tttgttcaga cctaaatcct
    13861 tgggaaggtt gctcagaact cgattaagac ctcgatttcg gagatacacc cacacagggc
    13921 tctcaggttt gatttcaccg gtatagtgat atatcacttc aagtttacgc cctcgttttt
    13981 caacttcatc gctgtcaagc gtttcggttg cacgaactgt tgatttataa cgacgttcac
    14041 tttcgagatc cgcatcagtt aatggagcgt acaaatccat cccaaagaaa tcagtcagga
    14101 tcttcttaca agtctgggtc ttgctcagct tgtagtactc tgccaataca tcaataccgt
    14161 cacaaaatgc attcactgga aaatcattgt gaatggcaca accggtaaag aggtcctttt
    14221 tacggaaccg gaactttgat ttcccgaccc cactaaatgg gcaaggaacc tggccaggtg
    14281 ctttatcaca agcgtcttta aatgccggta ttctatcgaa tacatagcgc cagcctgata
    14341 cttccgctat acgagcgtga accattttta taaagtggtc agtgacttca tgtggtgtac
    14401 gcttaacagc ctgggatgca taagtcattt gttctctctt atctggcatt gtgggtttct
    14461 caatgccctc tgcgatgcag aaaacaggga gaaacccaca acgcagaatc taagaatgag
    14521 ggcccacaag agccctcaac aattacgctt cagtcttgaa gccgatattt tcaagacgct
    14581 ggataaaacc atcaaccatt gacgttgcca tttctccatt accctctggt atagatacat
    14641 cccggagaat tctttcttct ggcaattcac ttttgacaat ttcacccatg cgaccaaaaa
    14701 tacggacacg atgcatcgtc ccgacttttt catactcgac acggtaagtc tttatctgag
    14761 tcatatattg ctccttaatc cttcagggag caaagcgacc caaccgggcg tcgttttgcc
    14821 ccgattgggt agagtttaat tagatagaat ttgatgtact aatttactga cctcattcgg
    14881 caataaagaa atatcatcaa catttacgaa ggtgccctca ttaaaaccca tcaacacatc
    14941 agttttaata ccaacaccag cgatttcaat gccgttactt tcagcaactg aaaaaacatc
    15001 attaatggtg taagccgact cagtcggata accatcagta ataaggaaaa caattttcct
    15061 gtcaaactgg ctttcaagta acaactctaa agcagcattt aatgcagaac ccgttggggt
    15121 attattgccc ttgcagccaa gtgagaattt tgaaagtttc tcttccgcgt tttcatcaaa
    15181 cgttttgata acttcaaaat ctttgtctac aaacggataa atcacattag caacatgcat
    15241 ttttgaaata gcttcaattg ccagagttaa tgcaaggtca gtcttaattg catggatata
    15301 acggtgttcc agactcattg aacctgaaat atcacgaaca atgaccaagc cgacgtggcc
    15361 ccggttttta gattcgcttt gtgaccgaaa tacatcccta acacccatcg gagactggat
    15421 cagtcgatta atatcgaatg acaagcctct atcacgggtt ttaaattgac cacgcatttt
    15481 tacctgctgt aaaacagata aatctgcccc aagggtctgg ctgatattta atgcttcatt
    15541 gtagacattc agatcaatgt tcaaatctgg aacatcccac tcacttgcac caaattcagc
    15601 tttaacctca tccgacacac tggcagcaat tacggagatc tcttttgcaa gaacctcatg
    15661 aaaatcctcg ctttcctcat cactgtttaa aaaatcatcc agcaatttgg caagcatatc
    15721 ccactgttca ggagaaaagt tactttcccc atcagaagaa tttccatttg aactgtcgtt
    15781 tagggaaggt gtcgagtcag catcatccga gccgggggtc gccgattcat caccttcaga
    15841 ctcaccatca ggttcgccgt tagagttctc agcattttgt tcatcccctc cggattggtc
    15901 cggtaaccca ttaccttccg caggcgcctt accatcacca gaatcatcgc cttgaccttc
    15961 tttatcacca tccccttcac cctggctatc accattgggt tcggtatcag gttcattctc
    16021 agagccagat gattcatctg tatcgtcatg cgggtctttc gactgctcct cttcttgctg
    16081 tttctctttt gcttcatccc ttaaacgctc taaaagagcc agagttttac gcgccagaga
    16141 atcgcaattt tgcgtacttt tgcaacttag agcttgatga atgatctcat cgacttctga
    16201 agttacaggt gcaaggattt tctttgcata atcgaaaaat gggtcaagcg tttctttatg
    16261 cccttcttgt tggagaacgt taacccgcaa tgtattaagc aggtacattt caataaactg
    16321 aaccgggttt tgaggggttg aagaaatatc aaccgtcata cgcccagctt caaccatcaa
    16381 ggcataagtc accgcgaggc gtttttttgc ctcctgataa tcaatacccg tcttctcttc
    16441 catctggaca tcatcaaaaa gattaataag tccttttagt cgacaagcac gagcatatgc
    16501 tttttgcttt tgttttaggt tttgaaaatc agccttcaag tcatcatcaa tactgatgaa
    16561 gccatctgca ttgatcaatt ctccaacaaa agcttcacgg tatacttcat gctctgtata
    16621 acgaccgtgt cctgcttcat gacatatgaa tccttcaatt agcttgacaa atctctcatc
    16681 gctataatca ccgttaggta aaacacagac attgttttgg gtgttgaaat aaggtgtact
    16741 caccttattg tcaataatta cataaatccc atttataccg cttatcacgc tggctatatt
    16801 cgtcaggcgt tgaatatggc gtggtgaaga ttttaaatca aatttcattt gagtaccttt
    16861 atttgataaa gggtactcac ccagatggtg aagtacccat ctggggatat ttttacaaga
    16921 ctaaattacc aaccggtcaa ttcgagttcg cagtcaaatg ctctattact accactggcc
    16981 tgaatagaaa catcttcatc agggccaaca gcaggctcag gtgcattcac tgccttgttc
    17041 tcaagatctt caaccgggtc ttctctgaaa acatttgctg aagaaaaaac atcatcctca
    17101 gcagtattca gagagttagc cgctgtaacc tgtgaatcga tattgaatga ccgggttatg
    17161 gaatccagcc agtcggttac cttttgttca ccggagataa tacgtttcag cattgacgca
    17221 tcgctcatga aatagaacca ctgctttaat accgcaacat cacggggaga agaaatggca
    17281 ccttttggta aattattcat gatatgctta atagcttctg caagaacaac agcctcttta
    17341 tgaatgaaca tgaagctaat tattttatcc tgcatctgct ttaatcggtc aatcgtgctg
    17401 gaacgcaaat tatcattttt gctgattttt gtataaacct tcatagcttc atcggccaag
    17461 tcagttatca gttgttcttg ataaaagtcc tgcgtttcat ccccctccac actaatcggt
    17521 gaaaacggaa tgaaatgtgc cagattaaac cggaatgatt taacaaaatc atctttagtc
    17581 aatttcaact ggtcaattaa atctgacatc aacggactgc gatctttctc agcctgaata
    17641 tgagtgtcat agttatcaac cacgttctgg ataccggctt gaaattcacg ttcgatttct
    17701 tgtgcctctt cgagaaactc ctccatcctt tcaactggca gtatccacat attgctatag
    17761 cggatgcaca accgtgaaca aagggttgaa attcgactag tgatagtgct tttgaaactc
    17821 agtgccttta tgtctatcca gcgaacttcg cttttagaca cgctcttctg gtctgttata
    17881 tcatcaccgc cataggacag ttcgatttta tcagttcgcc gagagccctg acacccacta
    17941 atttcaattt ttgttataac agcaccttcc tgcaagtcag gatatgcttt tataaagttt
    18001 tcactgattt cctttgcaga aactttatta agtttagaag tattgaaagt cataggtttt
    18061 ctccagattt agaaatggga aaacctcccc gtcgggaaag atttcccgat agggttaata
    18121 atattacttt tcaagtgttg ttaaaagaat tccaggaaat tcattttcga catccgccga
    18181 ttgaatgctc ccttgataca tgaatccttt tgattcaagg cccttaatca tggcttccat
    18241 atctttgtta cagacttcat aacaaataaa tgaatagtca ttattatgct gcttcagacc
    18301 tatatattga tttccatctt gtgcaatcac gaaagtttca gttccgtttg cactcagaaa
    18361 accgtaaaac gtaagatgag caaagtctgt cacacggcat tcagccttct gataactcgt
    18421 cccatctata actacttttt tgcagtgtaa tactccattg tacggttctt gtcgtcaccg
    18481 agaattaagt cgatggcttc caacaccata ccagtggcat gtgcaggcag agccgacgca
    18541 aaagcaattt taaatgtatc cagtaaggac atggtcttta acgtcattga cgttccaacc
    18601 catgccacaa gctggcgtat atccattaca ctgtcgtcag ttcctgtgtt gtttactgca
    18661 acggctaccg aaacgaattt ttcaattacc agcataggca ggttattgta gcgtttaagc
    18721 aaaacgttgg ttaatgcaac ctctgaaggc ctttccatct ccacaatcag gaagcgcttg
    18781 ataaacgctg catcctgccg ctgcgatgaa acgaacttac gagatccccc actcatgttt
    18841 gtgtttgaag tacccataaa ccgcgtaaac ttatttgcct taaccacttc gccaccgttg
    18901 gcagctagtg accagggctt tcgctctata ggcatatgaa gctttgcggc agtatcaggg
    18961 tcaatcttgt ccacttcatc cagaagaatg aggtggcctt cctgaaatgc cgttacaagc
    19021 tcgctaggca caaaactggt gttaccgtta ataagactac gctcaccttc cagctcatca
    19081 gcgcgaatat tgctgttgat ttgctttatg gttagcggcc agttcagcat atggctaata
    19141 tacattgcca tttcagtctt acctgatcca gattctcctt tcaggcatac actcaaatct
    19201 atatcgttac ttacaagcaa ttttactact tgctcaagta ttcgagaatt tggtatatag
    19261 tccttattca gattcgcacg atatggagaa aactttttaa acactggaac aatcatatcc
    19321 tcatccatct caggaatgcc aaataaatct tttgctttga caagataata ctgttcttta
    19381 ttcgcaaaca tcttacttga tataacattt gcgggtaaca gattaccaat gatttcttcc
    19441 tgtacggaat tgtcggattt taatacagtt gcggcgttag tcatttttaa aactccggta
    19501 aaatgcaaac accgcccgct ggcagatgtt tgccagtggc atgttagtgg aaagtcatcc
    19561 tacacgcggt cgccatctgt gttctgatag cggcaacgtt gtaaccaaga tgagttccta
    19621 cgatttcgaa tacttctgac tcaatccatt caaactggcg ttcaaatgta gggtttacga
    19681 ttacccaacc aaaagtatct cttaaggtta acttgttatt aacgacaagt ttttcaaaca
    19741 aattaattgt tgatttaaat actttagaat agacatccat tatatcccga tcagtccatt
    19801 tcattgccag tttgttttct tgcaacaaat agtcctgttc gaaaaagata tcccatccca
    19861 tctcttcctt acaatcatgc agaataacct ctttatcttg atgataacga gctaattcag
    19921 cattttcacc tccgtaactg tgcgtatatt catataagct ttttttaagc tctgacctga
    19981 actcaatagc atcaatacca tgagtggcac atgtaagctc aaatgatttc gacatatacc
    20041 accgtgtttc ggcttcaaga agcggattca cggttatgtt accgaattca tcagtggaat
    20101 agagtgattt tgtaatgacg agatgcctta cgttttccaa cgactcttcc attgcgacga
    20161 cgtataactg atatacatct tcaggggccc agtcaaaaac cagaccacct aactccgcac
    20221 tgtactcttc tagagtgaag agataatcag tttccgcagc aaaactaatg tcatgcagaa
    20281 tttgattttt gagtagtttg ggacaattac caggcaaagc aacatcttcc gacacactag
    20341 gctccagttc cgggcctgaa tgtttatctt ctgattgaat actttgttgt gcggctattg
    20401 catcatttat atagtcaagt ggagactcaa ctatagttat tatttctttt tcctcttctt
    20461 caaaatcgaa tatagagaaa aaatccattt gcttactgca aaatgcagtc ggccttattt
    20521 tttgtttcat gggatctcct aagggagaac tcccatgaag ggatttctcc ccttttgggt
    20581 ggtttaaatg tcagatctgt ctaaagctac tttgcccaaa acccttagga ggtttaggta
    20641 tgtgagctaa cttacttgtt gcaactactc tcatgtagtc aagaggatgc tctctccctt
    20701 ccaatgtgat ggtatccaca acaagataac cggagaccat taactcatca ccttgattta
    20761 taccagtgaa acagcctgaa tacatctctg gtgatacttt gatttgaatc gaaaattctc
    20821 tctcagtaat ttcaccatta atttcagttt tcattctgtt tttgagcttt atcaataacc
    20881 caacgtttcc gctagtcatt tctttctcca ttgcactgat aactatcccg gacactttta
    20941 cttcattaac atattcaaga tatgacatta cttctctcct caatttaagc aaagagaagc
    21001 cccgtcaggg tttcccctta acaggattag ataaatttgt ttacaatgaa cataaatgtc
    21061 atcatggcaa taacagaaaa gccaaacagc tgaccaaaag atcttggtgc ttttaccgtt
    21121 ttagcgttat tgatttgtgc caagagttca tcaactgtcg cttgccgttt atctgctttc
    21181 atttgataat ctaaacattg ctcctcgcgt ttagtgagaa actcaatttt cgacttgata
    21241 gcataatccc agcctttgga atcgaaaacg ctcaattcac taaaggtgtg taacatgagt
    21301 tcatcaggaa aacctaactc tttcattaat gggcgaacgg ctgcatacat tgcatctaca
    21361 gtttcataat gtgctttggt cacttcatta aatcgattac gcaatgctaa tttctcgcgc
    21421 ttctcaataa tttcagaaac ccaggcaacc aactgatcat tttttaactc tctggattta
    21481 gcgagtgaat aatcaaggtt atccacatca agaaatgtgt tcaacccgta cttttcaaac
    21541 tcttcattta agaacttatc cagatcggct aaagttttaa acattggaat tgcaggtgct
    21601 tctttgagaa gtgtgtaagc tttctcaaag tcaattggat tgacaagaat ttgctgcatt
    21661 tccacatgat aataagtaga aaaagcagaa agcttttgag ccagcattga aactgatttc
    21721 aaagaattca gttcaattat ggttggacca tcttcattca taataacaat aaggtcatta
    21781 tcatgccctg aataaacatt cagagaatcc aataacttct tgttaaccac cttatccata
    21841 tctgggatca gatcagcgaa ggcgatgaga agagctttac tgcgtgcttt acttttagac
    21901 atttggggtc acctatatat agtaagaagg aaggaatcat taaatatccc aacacaaagg
    21961 caaagcctgt cattcatctc ccgtaagcta tgaaccgcct gatttatact ttctggcatt
    22021 agaagtacgc aacgaaaata gctcaattaa gagctaagtt agttacgatt tggtaaattc
    22081 gattgtcctt tttcagacat gggaaaggca ccagcgacaa aggtgacatt ttgccggtgg
    22141 tgccttgcag ctttagaaag ctggccgctt ttcacggctg aatatgcgct taacgcgcaa
    22201 agtaactttt ataaagttac ttgaaatgtg ggagcaataa attcaagctc atatacgtta
    22261 tactacacgg gttcattcct gtaaataaag aaatcaaaat tattctgtca aaggggattc
    22321 tgttctactt ttcgcagcct ctttcaattt atcttcagca cattcgctta aaaagaaaga
    22381 cggatcaccg taccacatta catgcagctg ttgctctgct cgtgtaaacc caacaaaaag
    22441 cagtcgtctt tcttcttcaa tcgcctcctg ccccactgac cgcttacttg gaatctgatc
    22501 tgcattacaa ctcataataa ttacattttt ccattcaaga cccttcgaac cgtgcaacgt
    22561 cgataaaact actttatccg gtgtatattc ctcatccttc ttctttggcc ccagacacat
    22621 cgccgcagct cgatttatca tattttgcca gcctgtcttt tcggcccatt gcttacaggt
    22681 atccagcgca attctgctca gtgatggtga tcctttctgc gtcctcattt ttatcgacga
    22741 caactccatc cacttaatca gatttgcgaa ccttttttct atttcaacct tttcccgagt
    22801 ctcctgtgta aaccgggtga agtttgaatg aagcgttctt gtttccaggg aactttcatt
    22861 cgggatgttc agttcaccaa aagttacttt tcttcctttc atgctcaatg ctgtctggtc
    22921 caagacctct tcattttcac cgaaaagcgc cagcaccctc ttcattaacc ggacatcgtt
    22981 ggaatgtcgg aagaatgcca tcaaatgcaa tacatcgctt gtctcctttt catcccaaaa
    23041 tgacttccca ccataacgaa gtaccggctg ctctatcagg ctttcaagct gatcgagatg
    23101 tgcattacct ctgctgagga ttgcccaccc aatcgggtct tggttaatca gattgagaat
    23161 gccttgaatt tgctcatcca tatcaacata tgagcggaaa tgaacttttc ccccaccgcc
    23221 cttagctgaa cggagatctt tagcgtatct gtaaacattt ttttcaatca atgcccctgc
    23281 taccttcaaa atctctggtt cacatctgaa acaagtattc aaataaaata tgttgggcct
    23341 gaattgtttt tcaaactgct ggaatatttt gactccacct gatgccctga atgaataaat
    23401 tgcctgatca tcatccccaa cgatgctagt gttaacacct gccctcgtat gtagtgagat
    23461 ccaggcatac tgaatcgagt cggtatcctg aacttcatct acaactaaat gtgtcaagtt
    23521 aagtggctgc attttccccg agtacatctg gcccacaaca aaacgtgaga gtgcattcaa
    23581 atctgcaacg tggtctttct gacatattgc ctgataggtg ttgaacaagt ggatttgttg
    23641 ccgcgtatgg cgaactgaaa tgatgtccgt atccatttcc cttccaatcg cgtcaatagc
    23701 aaattctgca tccgggtatc gaaaaggcgc gccggactcc ttcaatgccc tgtgaattac
    23761 agaacgctgt gccgagctaa tcaacatctt gcccttccag ttgaccatat ccagttgcat
    23821 ccggataaag ccgtggaatg tattgaccag caccctatcc tggtctttct tacttaatcg
    23881 acgtttcagt ttttcagcca atgaatttgt tgctgcacgg gtaaatgtga ccatccccac
    23941 tttggcataa gggaactgcc tgaggataga gatcaccgcc tctatcatcg tgaatgactt
    24001 tccagatccc ggtgctgcaa taaccatatt attagcattt gcattttcaa tgatgaccct
    24061 ttgctcatct gttggtttac ccataaaagc ctctgaatat ttagtcccct ttattatacg
    24121 atttaaattt gacggatttt tctttacaag actcattttt ggtcacttca tatttttcca
    24181 ttttttcttc atttaggttg tttgagaaac tggcgagtat gaaataatgc ttctagacat
    24241 aaaattcatt caaaaagggt cataaatgaa aaataaacgg tttgtatcct tggtttcagc
    24301 tttagttgca agtgcgtctc tggtagggtg tgctgggaac gcaggtcaat cccctaactc
    24361 cgcccagcac agcgacactc atgggctcta ctggcatgct aacgcaaaag attctgtagc
    24421 caaaaatgct tactctctgg ccgggtttga cgtttctttt aatgaggaaa ctgttcagcc
    24481 aggtggtgat gcagataact tcctttccaa actcttgatt agcggctcta tgggatatgt
    24541 gacaggcggt ctcagcggat taagcattat gagtttgggc tctctataca gctcctctga
    24601 cgccgaatac attcaggtta accagtatgt tgtctttgtc ccgaacccga agaaacttcc
    24661 ttataacgat gaaagcctag ttcgtgccgg tgctgcgtat gtttacaatc acaccaaaga
    24721 gtcacaggta atgctcggat tcaacccatc gaagcaaagt gcagctctgg cgtcctgcaa
    24781 gattgatagg gcggtcatca ataaatggag tacatgcgag ctcacttcaa aaccagctcc
    24841 tgacgtaatg ccaactaatt ccatgtacag cttccaggct attcgccctg cgactggaac
    24901 tgaaatcccc caacttaatc tacctgccgg tgagtattca gttattcgtt acgtgttcat
    24961 tccatttaaa ggcaatgagt caagcaataa tttctcaggt attattttcc gttctgatag
    25021 tccaaagttc accatgccag gcggcgcagc tgcttcaatt aatgggaaag actattacct
    25081 cttctctggt gagtatggtc agaaaggatt cccggaaaaa actctgaaat caaaataaca
    25141 aaaggggctt tacagcccct tttttttaat attttggtat ttccttacct gtaattggat
    25201 ctacataagt tgacccaact tcacaaatcg cagggcgtgg aagatagcta cagtctacgt
    25261 tctcctcttt aatggcctga gcgttttcct gtctggcttt tatcatattc tcctctcctc
    25321 ccattgcatc ttttgttctt tctgatggaa gttgataatt ctcgttttta aatggcaaat
    25381 ttgctgttat tgacgattct gagaatgtag cgtactggtc cggtacttct ccgacctgaa
    25441 cagcatcgtt aatccattct gttaaatcaa tcttgtcaaa atctaacttt tggatctgcc
    25501 ctattgataa gccaacgcag tttgaagtaa ccgggtctat tccaagctgc gggtaggcct
    25561 gctgcataat aacgcgagat aacattgaat tgtaacagca catgtctgtt gcttttttaa
    25621 cacatttctt tgtaaatccc agattcagat atttaacaca acggtttgta tccagtgtaa
    25681 aacaggattt ctgatttttg tgaatagatg cttccatttc tttttgatcg caggccacca
    25741 gcatttcaag cagcattttt gtgacttgat atgccgtata aatccagccg attacattta
    25801 atgcaagcat aaagttttga acagcagcag aaaagactat ttctccaccc gttgtcgcta
    25861 catttttaaa tacgaagtct cgaactgtat cgggaagcag gtcataggct ttttccatgg
    25921 ccatctgctt aagtttacca agcccgaatg aactcatagt ctccttagca agctgaccac
    25981 tttcaccagc agcggatgat cctactgttc ggccaaggtt accggccacg ttttcgtaaa
    26041 cgctggttat tgaactggtc acattgttcc acatttgtcc gacagtttta cctgccgatg
    26101 cagtccattc acccactgag ttagccattg atgcccaggt tccgctctcc cctccagtcc
    26161 agtttaccag ttgagcattc actcgggtcc agttcgtgtt tcggatcatc gacccggcca
    26221 gcattatcgc ctccagtgac ccagcagcac cctgcggctt ttcgcagcaa tctgttttgg
    26281 ccagtccgtt cacaaaacca acggacctgc cgcaccactc tgctttcccc tcaaagacag
    26341 agcaggagtt agggtcgttt ggatcttcgc attttgcttc accttgcatg tactgaacca
    26401 tattggagta cgcggccact ttcccaaaat ctttgttttc ttcttttggg cctgtttcac
    26461 atgttccgcc agaacatggg atcataccta cacaggagtt agtctgtgac tctacctctc
    26521 tgactacgtc tttccctctg tcacatgtat atttctgctc atacatgtag caagtatttg
    26581 tgccttcatc gaaccaacct tcagcacatg tctgattatc tagcacacac gcagcatcat
    26641 ttttatatgt gctgcatgcg tcaggcatat tttttatatc gtcgtagttg tagcaaaggt
    26701 ctttgcctgt ggggtctttc atgatgtttc ccttcgcatc ataagagcac agtttttcct
    26761 tcgccagggg atcacagaaa tagccttcaa ggtttgtttt ccaggttaag tatcctgtat
    26821 cacctttgta aagcggtgtg gccatcttaa gcacactttc cgggagtcgc tttgtaccaa
    26881 catccatgtt gacaaacctg tcaaaccgac agaatgaatt cggcgtttgt actgcatcaa
    26941 aacatcctgc gggctcctgt atatgtttat cctccaactt catattttca aaagtcatct
    27001 caacttcaaa atagccttcc ccaccaccgc caacccatac gcgcgaagcc atagtcactt
    27061 cacgatcctg ctcctgatat acagaggttt ttacttgttc agtaatatcg tagactttat
    27121 cgagcttcca ggatttcttt aactcccatt gaccttgtgg agatggataa ttgctacttc
    27181 tatatgctcc atctatatga gcaaaaatct gtgtaccatc aagcgtgacc ctcatatggt
    27241 catcccactc tgcgttaatt attttcgcag ttgcgagctt tgcatctgga tggaatttta
    27301 aggttaatga gttgtcatag actccatcgt tccaatagtt gtcgtccctt ctgccaaacc
    27361 agactctcac acaattatct ccgcacatgg acatatcacc attaccacct gagatataaa
    27421 ccggcacgga aaaatcacga gttatcgtgc aactgttaaa gttatctttc ttgggtgaat
    27481 tgcagtaaaa ctctttgtaa tctggatagt gcgtagtaat tgtttgtttt gatgtttccg
    27541 tattacaatt ctgtagccag tttcctgtac cagctacagc atttcctatt tcgtttcgac
    27601 ccgcattgaa catagggtcg ttaggtttaa tagtagatgc tgggttttct ttatttgcat
    27661 tcaccagagt acggtaggcg actccatcat aactttgcgc ttcaaacagc tgactttttc
    27721 gatttttgat gtcattgatg aagccatttt catctccata ccggccacca gcattgaact
    27781 gaacatcact gtgcttctgc tctgagctga atagttcccc tgtactggtt ttggtacctg
    27841 ccgttgtatt cgggttgtta cttattccaa tattaccgct gctgtcccta gaaagtaact
    27901 ttgccccctc tttcgtataa ttcgagttaa ttgtaccgcc cgacatgcta ggtaattgta
    27961 cagccttacc gatcgatttc ccgtcggaca ccgcattact cataacactt tcatttgagt
    28021 tcggcgtccc gtacttccca acatttaggt ttgttgactc tgctttacca acatatttct
    28081 cggaaatttt gccgctcaga ttttccccgg aatcagcatt tttatacttt tccacaatat
    28141 cctgagcact ggtggttgcg ttccggttgg cgttgggatt ggcaaggtta aaattgtttt
    28201 caagctggtc aaaaatcgca tctgccgcaa atattttagg cgttaacgca atgtttatga
    28261 gcgacacgta aaatgcccaa ctcatagtat tagctatcat agacgcaata attgctgaag
    28321 aaattatttt tctatgcttc atatataata ctcaattttt tcaattagtt gggtatatac
    28381 ctgacgcagc aatcattata gcgccacagc atgtaaacat agttatctat gccgattttt
    28441 ttccgtccac ctttaagcgt tgaccagtca tggacagaat cgcctaggcg atgacagcaa
    28501 tccggaccat tcgcctccgg cgttgggtaa agcattgaaa gtcggtattg agatcgagga
    28561 gtgaagtatt cccacgtttt ttcacacatt gcgtcagctc ccattgttct cataagcatt
    28621 ccctgcctat gtaacttcgt cagtagacgc tgagttataa gtgagctggt tctgacggca
    28681 tcatcattcg catagatgtg tccagttaac ggataaaggt tcccatcaca cccggcacaa
    28741 aaatatgcag ccaaattatc cttcccggca gtcacggcca cgcagtcagc tgcacatacc
    28801 attgaggcga tagggtttgc aaataccaca gcttcggggt ttagcacgag cgtcaatagg
    28861 tcactttccc acatcggatc tacttcgctg atatacgtca gactggcatc aacataaggt
    28921 tctgctgtac attcaccaat aaccagcaat ttcaacatcg tcatgattgg gaattcccag
    28981 agatgtacgt cgtagaatcc ggcttctgtt gcggtcatag gctggttagt attgctcttg
    29041 ccgatgtcga actgtgattt ttgcagcatg gttccctcta aaaatgggct gcaccacgga
    29101 cttttcacca cttcaacaat ttttactggt tggaaaaaac ttagctgcca tccgatttct
    29161 ggtatcccat tttgatccgt gcaatagcaa ccagggcgtg agggggcggc accttcaggg
    29221 gctgctccaa ttcccatgat ccgcatcggg aagatacaac tccagcagat gtttttgatt
    29281 aggccctgcc atactcccgc tgactggcac attgctcccg ggacaccggt atctgcggcc
    29341 attgtcattg caggcagcga gagcgacagc atgacagcga tcaatatttc caaaagtaac
    29401 ttttttagct tcttcaccgg cacgctcact atttagtaat gaatgttgtc acgctttcca
    29461 tatacgcaac cactttgtta aaacactctc tcatctggtc cgggtaagtt agcgtcagcc
    29521 aagtggccag cagcgcccca aaaataaatc ctattttcat atcaattctc ctctgccgcg
    29581 atttttgttc caataccacg tatttcagct tcactgaatt gcgttatacg gaatttctct
    29641 ttttcagtgg agatcagcgc tggcgtgttt tttatctgga aacgagcaat aatttgctca
    29701 ttaactttga acaccttccc attgaatttt tcgcgcagat cattcaaatg atcccatcca
    29761 ttttcctgtt tgatgcgcgt gaacatcggc atcacctgac cgctgccaag acgctggcga
    29821 tattgcttct ctgcccactc aagttgccct gggttcattg ggtcaaaaat tatcattgtg
    29881 aggttctgcg ggaacctggc cagtgggtta atgagctcgc cagcagaagc aatgacgcga
    29941 ccctgcttat ctttaacgtc ctgagtaacc ctgattgtcg ggtcgattag ccattccttg
    30001 tgctcttgag cgggaggcag attaaccatg tactggtttt tccaggtatc agcgactgct
    30061 ctcttcttgg cgccgtccca gtcgtatttc tgtgctcgct cttcgagctc tttaataatg
    30121 ctcttctctt caaccggata ggtcgtacca gcaatcggga aaagatcttg gtcgggtttt
    30181 ctggccagtt cgcgctggaa gaaatcaacg ttgaaagagc cgaccatttt tgcaaaccgg
    30241 gattgctcac gggagattat tgtcgggacc tgcgtgatct ggtactggtt aaatgcgtcc
    30301 gggtcaatcg taattagtgg ggagtcaaac ttccccttca ccaccagctg gttgatagct
    30361 gcctgagttt cagggaggcg tgtagtgcct ttcttcagac cattaaaaac cactctcgcc
    30421 ttatgttctg acgcggtacg gagtagatct tccaggacgt aatcaggcaa gctaaatgac
    30481 gcaaagatag tgataccgtt ttctgtcgag agcacctctt cccgactgct gtctatttta
    30541 cgctcgaagt cagcggcggt gattcctgat gaaccaaaca tcttttcgat attcgaagac
    30601 tgactgcgca taacttctgg tttattttcg acggatttca gtgcagactc gctgagctgt
    30661 gaatttccct gaatctcatc acgtctgcga atgatgtcgt taactgcttc cgagttatta
    30721 ctctgagcgc ggatagcctg cgtatggtct ataactgcct gcacactgtc agcattcata
    30781 cccgggacat aaccgcgatt tgtttgctga ctgaggtccc cgtttttctt catagcttcg
    30841 gcctggcgga tgtatttctt ggtatcagga gaatccagag aaccagacgg gagcggacct
    30901 tctgatgcgt gtggtaccgg tatttgggcg cccagaacct gagcttgcct tgcaatgtca
    30961 tcagcaacct gttgatactg ggggctagcc acagcggtgg atattaaggc gtaaaatgcc
    31021 agacaggagg ccagaggctt aatagttgtt tttaattcaa aagatcgcat atgttttccc
    31081 aataactgaa gtcaacttaa ccggccccca aaaacgtgag tcccatgagt ggtccgtttg
    31141 cccaattaaa aagacctccc cttccggaat agttaattct ctttcaatgg tctcggcagg
    31201 tatgccgtat ttcacagaaa tacgtctggc attggcttta taaaatttgt tcccattata
    31261 aacaccatcc aatgtgaccc tcaggcgatc tccgccgact cctgccacaa ttttaataac
    31321 cttctcattt tcacgtataa aacggactga ttttggcata tgaaaagcta tgatattccc
    31381 tgaacttacc gatttatcct tcagatcgat catgtaaaaa tcagcaggta aacatccatt
    31441 taatagtcca tgaccaaacg taaatcgatt aaaaaaaatc aacataacca gtatcaggat
    31501 tccgagattt attggcacgt accaatcaaa actcagtaaa ccacgcttct taggtatgta
    31561 ttctctcata acctacttct ctcagaatca gaaagcaaaa tggactgtct tttacattac
    31621 cttaaaacct attatccatg ttttggtact tccttatttc agcaaagcca gctatttctg
    31681 attatttacc gccttttttt ctttcattcc gtctttgatt tgtgattgtc ctggccgctg
    31741 tcttactcat gtaaagtaac tttcccgacg ttaccccatc aaaaagtgaa aaacaaaaaa
    31801 gccgggacat ttctgccccg gctgttattt cattagcttg ccttactgcg aggtttcaac
    31861 tgaatctacg gactttgacg cgccctcatc cggaccataa acggtcgtac gctgagttgc
    31921 tgaatcccca ctatcatcat acgcgacaac tcgtatatct tgcttcccac gattaaggtt
    31981 tttggacagt gtcgcagtgc tgccgaaaac attccttagc tcatcattga tataccattc
    32041 atatcgaacc agcttgccat cagtgtcaat acatttgttt gtgaaactcc agctcatatt
    32101 agactctgtg tagcttaatg cacattgcgg agccgcgttt ttaacaacct caaaatcaac
    32161 tgatgcggta cctctttggc ccattttact gacaacatcc agcgtgactt cgtatttctt
    32221 ctcagtaaga ccagaaatta actgagccca atagttttta ctaccaggaa caacctcgcc
    32281 gtttactttg tatgtcacag tatcaatact gtcagcagag tgcgccagtt taatgttaga
    32341 tcttagcgtg acatcaagag gcgccctcat gaacttatta gagaatttcg gtgtcatttc
    32401 tacagtcatg ggcgtttgct catcgactac gaaggtattt tcaatacgct gttcgttgtt
    32461 acggttatca cggaaaacga ttaccaacgt atatttcccc ggcttcgcaa ttgcaaattc
    32521 tttcgtatca ccatcatctt ttaaaactga gatattgtcc ttatcgtaga tccattcccg
    32581 agaataagtt accccaggat agccaccgtc ttttattccg ctcagcgcaa catgtaaggt
    32641 cgacggggcc atggtgtatt taagctttgt actgactttc atctcaggaa acttatacac
    32701 ccaagattta acagatactt tccgcgtgga aatagtactg tccttactgt cagcaagcca
    32761 tgcactgtat tcaaatgccg catatccttg cttatccagc atctgctcag tcaaagttat
    32821 cgacagattc gcacctctga ttttttgtcc gtcaggggca gtccattctt caatgacgtc
    32881 atttccgttc ttgaatcggc tgttcgggtt agtgtattta ccatctaaag taactttact
    32941 tcccacctcg acgcttgaaa taccacttac tgaaacagat aggcgcttgg gtttagcgac
    33001 ggttaaccgg gcagacgtct ctttccagga tgactcttca gccaactcat cagaatccag
    33061 gtaacgcatt cgggcgtaaa tggtcggagg tagcgtatca gcggccgata cgttataggt
    33121 agtgtcaccc tgaacccaat tcactttatc ctccgaccac atcacttcgg ccgtaccctg
    33181 cggaattggt tcatcgttat ccagcagcgt gacaggcaaa tcatcgcctt cgagaatttc
    33241 agcaacatcg atgctcagct taggctgttt gtacgttaca accgtcagaa cgtcgctata
    33301 agactctttc gacgtaaact tgttggtcat ttttgccctg taaagccatt taccaacctc
    33361 agttttcctg atcgaaatgc ttcgcttccc tgtcatgtcc tgaagcatag tccatgtctg
    33421 tccattatca gaagactcct gccactcggt aggcgccagc gcatcggagt tagctgtact
    33481 ggccgatttg aagttaacaa gcgtactgag cgggatacgt ccgataatca gactctttga
    33541 gagattacct tctagctctt cgcccttata aacacggatg ctaactgcac tggtttcccg
    33601 ggtcagttcg aaatctgcga aaggcgatcg tactttagca atcccgacaa gtcgcacgtt
    33661 cccgaggtcc atattttcta tcttgatctt ggtttcaacc accccggcat cattcacggg
    33721 cagtgcttcg gttaggggtg tacgttcata cgtcgtttta tatcggcctg tttctggatc
    33781 ttttccataa acacttttct gagcgtaaat ggttacatcc cagtccccca ttgttgtccg
    33841 actaaatgaa cttttgcggg ttgaattgtt atagcggcca acagacacct tcaatggcaa
    33901 ttccatcgtg gtagcagtct ttttgtcctc aatttccatt gccgctctaa tgttaaagtc
    33961 gggtaccgta tacacattga gcgtatcaac ggttcgcata gtcggttcct tcgagtactc
    34021 gacagctatg tcgaagacat tcttcgtcca caacggggct gatgccactt taagacgttg
    34081 aacaaacgtc gttgcatcgt tatttgatga acggttctta aacgtcgttt ttacgctttc
    34141 ccccccgttt tgtgaaacat agactgtcac atcagcagga accacttttg ccaatagacg
    34201 agctgcttcc ccgccagtgg tatacgccag tgcggtgtta tcgttaatcc ccgtcaacac
    34261 cttgtttttc ttgtaggtaa ggaccgggtt ataaggtaac tgcgtaatca gcacatggcg
    34321 gtttctggac gatacagctt tagagccctt gccgtttatc atatagacct gatagtcgag
    34381 caggttatcg cctgttttat tgaagaaccc tgtcaggcct ggcgtccggc ctttaaagta
    34441 aaattcaagc ccgtcaggca cattgttcca ctgagggtaa caggcggtga tatcagattt
    34501 gtcaccatac cattcaaggt atgccagtgc gttctcttct gtagtaaaga gagtgcattt
    34561 atcaccggcc gtctgactca tgagatcggt atactgccga actcgtgagt acaatgtgat
    34621 gtctttctca aagtttttaa gcgtgaatcc tgttggttga tacgtgaaag tagtattaaa
    34681 tgtcacaatc ttgccgtaca ggttttcagc tatgaccgtg atttcatggt cagcattcac
    34741 ttctagatca tccccaaccg gcagataata aatgcctttg tggttcgtgg gggtgagctc
    34801 aacaagtttg ttatctatcg taacagcttt aatatccgcg actccggcct ggccctggaa
    34861 tgcggcaaca ataatttcat ttaagaaata cacaggagct cgtttggcca gagctgcacc
    34921 gtccttaagc accatcagtg ttgggttatc agaaagcatc agtttgcttt caggctcatt
    34981 gtacgtagag gtattcttgc tattgttctc taccgtgaac gaggcggcat aagaaccatt
    35041 cgtcaagtct gttaagtcaa agatatacag atattcatcg ccgactttct gtggcccgct
    35101 cacgggctga agagtacgac gtagcggtgt tgcccaggat tgcttaaagt cgataaccgt
    35161 accatcttct aaagtaactt ttgttgtcgg cttaaacacc ccatcaggat tcatcgttag
    35221 cgtcatagcg gttggtaacc accactcacc ctcagtttct tcctgaggca ctttgacggc
    35281 tacggccacc gttctagctc ctgtatagat ctcctgtcca ccaatactta ttgttttagc
    35341 ttcatccgtg aaattccaga gagtatggtc aacaacagga ggacggtaat cccaggtcag
    35401 cgttagcttc tgcgcttcag gaggggcaaa gtaattattt ttcgaattgg cagtgaccgt
    35461 gatttcccgt gaacccatca gcaaatccga atcacttact ttgatgcttc caacatcgat
    35521 cgtgcagcca ttcccaccaa ttgcgacttc gcaagctgat ccagacccga tagagatata
    35581 ttgcacataa ttgcgaggct ctacggagac agtgacattc ctaattgctt catccttaag
    35641 ataggattta agagtgtaac catcctgaac cactccattc atagacgatt tcaggcccgt
    35701 tatcgtcggt ttaaccggta caagagcatt aactgtatac tccccattaa ccacttctac
    35761 tttctgcctt tctgatccag aaaaataaga aattttatac ggcagtttta gttctcctgc
    35821 cgcattgggt atccctgtta aattgaatcc gtttgctgta aagccagagg tatttggaag
    35881 ccattcaaaa aagcagacca agttatctac gcttgatgtc gcacgatcat caaccgtcac
    35941 attgcatgcg ttgccagtac ctgaagcagt aattgtatta ttgttgacga atgcaacaaa
    36001 atcgcccggg ccgggattaa acgtgacggc aggcatatca gcctggagtt ttccggaaat
    36061 cataattgcc tcagcttgta ccgatttaat cgaagcaatt aaagaaaaca acagggttaa
    36121 acataaacct gattttctca tgacgaacac ctgttttttt acgcagaatt agaatcactc
    36181 tacgttaaaa aattctgaaa tcttgttcgt aaagaggaaa aaaaa
//
LOCUS       DAOFQD010000038        36028 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.038, whole genome shotgun sequence.
ACCESSION   DAOFQD010000038 DAOFQD010000000
VERSION     DAOFQD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.038"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            83..424
                     /locus_tag="QBF46_001925"
     CDS             83..424
                     /locus_tag="QBF46_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HDQ6674113.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGGDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="QBF46_001926"
     CDS             complement(415..1341)
                     /locus_tag="QBF46_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6674114.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVQRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1431..2429
                     /locus_tag="QBF46_001927"
     CDS             1431..2429
                     /locus_tag="QBF46_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDQ6674115.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="QBF46_001928"
     CDS             complement(2426..2644)
                     /locus_tag="QBF46_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDQ6674116.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="QBF46_001929"
     CDS             complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="QBF46_001929"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDQ6674117.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4732..5730)
                     /gene="zipA"
                     /locus_tag="QBF46_001930"
     CDS             complement(4732..5730)
                     /gene="zipA"
                     /locus_tag="QBF46_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDQ6674118.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQSVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5960..6721
                     /gene="cysZ"
                     /locus_tag="QBF46_001931"
     CDS             5960..6721
                     /gene="cysZ"
                     /locus_tag="QBF46_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDQ6674119.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6906..7877
                     /gene="cysK"
                     /locus_tag="QBF46_001932"
     CDS             6906..7877
                     /gene="cysK"
                     /locus_tag="QBF46_001932"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDQ6674120.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8261..8518
                     /gene="ptsH"
                     /locus_tag="QBF46_001933"
     CDS             8261..8518
                     /gene="ptsH"
                     /locus_tag="QBF46_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDQ6674121.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8563..10290
                     /gene="ptsI"
                     /locus_tag="QBF46_001934"
     CDS             8563..10290
                     /gene="ptsI"
                     /locus_tag="QBF46_001934"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDQ6674122.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10331..10840
                     /gene="crr"
                     /locus_tag="QBF46_001935"
     CDS             10331..10840
                     /gene="crr"
                     /locus_tag="QBF46_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDQ6674123.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(10882..11733)
                     /gene="pdxK"
                     /locus_tag="QBF46_001936"
                     /gene_synonym="thiJ"
     CDS             complement(10882..11733)
                     /gene="pdxK"
                     /locus_tag="QBF46_001936"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDQ6674124.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELETLTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11838..12212
                     /locus_tag="QBF46_001937"
     CDS             11838..12212
                     /locus_tag="QBF46_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDQ6674125.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCYEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12245..12979
                     /locus_tag="QBF46_001938"
     CDS             12245..12979
                     /locus_tag="QBF46_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HDQ6674126.1"
                     /translation="MKKRFIYHDEKSNKFWWIDYEGDSLAVNYGKVGSIGKFQTKEFD
                     NEEQCLKEASKLIAAKMKKGYQEDPKFNFMDRYYFDDEEIGLHVKTSHPNFQCHFTDP
                     LYMCCWDEESPFGSDEGADALNVLENSLRKEPDLDCADFPQMLIETMWGMKYIAMDSI
                     LEEDVRAQLLVDEMSTIQSNMITYATAFGQIKVMGKISHKLKKMGLNALARHQLTAKI
                     LQWGDGQDSPILQKMIDDLTAFPHEN"
     gene            complement(13168..14079)
                     /gene="cysM"
                     /locus_tag="QBF46_001939"
     CDS             complement(13168..14079)
                     /gene="cysM"
                     /locus_tag="QBF46_001939"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDQ6674127.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKREEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14213..15310)
                     /gene="cysA"
                     /locus_tag="QBF46_001940"
     CDS             complement(14213..15310)
                     /gene="cysA"
                     /locus_tag="QBF46_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDQ6674128.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15300..16175)
                     /gene="cysW"
                     /locus_tag="QBF46_001941"
     CDS             complement(15300..16175)
                     /gene="cysW"
                     /locus_tag="QBF46_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDQ6674129.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16175..17008)
                     /gene="cysT"
                     /locus_tag="QBF46_001942"
     CDS             complement(16175..17008)
                     /gene="cysT"
                     /locus_tag="QBF46_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDQ6674130.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSTLVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17008..18024)
                     /gene="cysP"
                     /locus_tag="QBF46_001943"
     CDS             complement(17008..18024)
                     /gene="cysP"
                     /locus_tag="QBF46_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDQ6674131.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(18328..19119)
                     /gene="ucpA"
                     /locus_tag="QBF46_001944"
     CDS             complement(18328..19119)
                     /gene="ucpA"
                     /locus_tag="QBF46_001944"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDQ6674132.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19248..20105)
                     /gene="murR"
                     /locus_tag="QBF46_001945"
     CDS             complement(19248..20105)
                     /gene="murR"
                     /locus_tag="QBF46_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDQ6674133.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSHKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELAVEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            20269..21165
                     /gene="murQ"
                     /locus_tag="QBF46_001946"
     CDS             20269..21165
                     /gene="murQ"
                     /locus_tag="QBF46_001946"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDQ6674134.1"
                     /translation="MQFEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIKHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21169..22593
                     /gene="murP"
                     /locus_tag="QBF46_001947"
     CDS             21169..22593
                     /gene="murP"
                     /locus_tag="QBF46_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDQ6674135.1"
                     /translation="MAKEISSELMNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMVVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            22598..23902
                     /gene="pbp4b"
                     /locus_tag="QBF46_001948"
     CDS             22598..23902
                     /gene="pbp4b"
                     /locus_tag="QBF46_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDQ6674136.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPIKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(23960..24859)
                     /gene="yfeX"
                     /locus_tag="QBF46_001949"
     CDS             complement(23960..24859)
                     /gene="yfeX"
                     /locus_tag="QBF46_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDQ6674137.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(24955..25530)
                     /locus_tag="QBF46_001950"
     CDS             complement(24955..25530)
                     /locus_tag="QBF46_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDQ6674138.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(25591..26040)
                     /locus_tag="QBF46_001951"
     CDS             complement(25591..26040)
                     /locus_tag="QBF46_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDQ6674139.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKK"
     gene            complement(26027..26452)
                     /locus_tag="QBF46_001952"
     CDS             complement(26027..26452)
                     /locus_tag="QBF46_001952"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDQ6674140.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            26666..27535
                     /gene="amiA"
                     /locus_tag="QBF46_001953"
     CDS             26666..27535
                     /gene="amiA"
                     /locus_tag="QBF46_001953"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDQ6674141.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            27539..28438
                     /gene="hemF"
                     /locus_tag="QBF46_001954"
     CDS             27539..28438
                     /gene="hemF"
                     /locus_tag="QBF46_001954"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDQ6674142.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGTEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKGGSPEAALSEFIKVRDWV"
     gene            complement(28444..29496)
                     /gene="eutR"
                     /locus_tag="QBF46_001955"
     CDS             complement(28444..29496)
                     /gene="eutR"
                     /locus_tag="QBF46_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDQ6674143.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRTREWG"
     gene            complement(29542..30042)
                     /gene="eutK"
                     /locus_tag="QBF46_001956"
     CDS             complement(29542..30042)
                     /gene="eutK"
                     /locus_tag="QBF46_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDQ6674144.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(30055..30714)
                     /gene="eutL"
                     /locus_tag="QBF46_001957"
     CDS             complement(30055..30714)
                     /gene="eutL"
                     /locus_tag="QBF46_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDQ6674145.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(30724..31611)
                     /gene="eutC"
                     /locus_tag="QBF46_001958"
     CDS             complement(30724..31611)
                     /gene="eutC"
                     /locus_tag="QBF46_001958"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDQ6674146.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCTTTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(31632..32993)
                     /gene="eutB"
                     /locus_tag="QBF46_001959"
     CDS             complement(31632..32993)
                     /gene="eutB"
                     /locus_tag="QBF46_001959"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDQ6674147.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            33182..33721
                     /locus_tag="QBF46_001960"
     CDS             33182..33721
                     /locus_tag="QBF46_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDQ6674148.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTDSLTPGLFLLVHPNGSKYWR
                     FRFWMDKKEYLQAIGVYPLISLKEARKRATESRSLIANGINPVEEARKEKAIDALNMA
                     AGFKTVAEDWLATRVSGWSESYTKQVRSALEKDVYPVLGKRSIVDITARDVLAPKFGA
                     MKSHRSCQAIPSTTQNAEI"
     gene            34184..34375
                     /locus_tag="QBF46_001961"
     CDS             34184..34375
                     /locus_tag="QBF46_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6674149.1"
                     /translation="MNDSELMTVNEVAALFGVTRRTIFRWMNKIKGWPVPVSPIGSRI
                     KFIRSEILEFYKNKGARHQ"
     gene            34515..34778
                     /locus_tag="QBF46_001962"
     CDS             34515..34778
                     /locus_tag="QBF46_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674150.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFQEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKARLLFELSRRFGVSISTFK"
     gene            34796..35338
                     /locus_tag="QBF46_001963"
     CDS             34796..35338
                     /locus_tag="QBF46_001963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674151.1"
                     /translation="MDAFDVLTNKGDELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIEIIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPTTDEYFSPD
                     AEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     AADPEAEQRKRVFNAIGIRY"
     gene            35477..35764
                     /locus_tag="QBF46_001964"
     CDS             35477..35764
                     /locus_tag="QBF46_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674152.1"
                     /translation="MLALRIMQGTAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            35803..36009
                     /locus_tag="QBF46_001965"
     CDS             35803..36009
                     /locus_tag="QBF46_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674153.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT         8799 a         9613 c         9067 g         8549 t
ORIGIN      
        1 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tggcgatgtt gcttcacaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga gacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
      421 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca acaaccggcg taatgcgctg taccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgccaaaa tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 gtgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt cagttcatcc agcagtcgct caagacgcaa
     1141 agccagctgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactc actgccgact ggctcaggcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
     1861 caccattgct ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcac
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaattcatc tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac
     2581 gccctttata tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgctt tcgccagttt agaacctgcc gcttctccgg caatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcaacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccgatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagccgc cagcggcgca gcgcctacac gttgagtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
     5341 ctgcacggat tgctgcaccg gctgcgctgg acgcggagca tgttgcggcg gtacctgcgc
     5401 ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gttggtactg
     5461 gtgttgcggc gacggacgag cagcctcatg ctcctgggcg ttagccgggg catgattcac
     5521 gcggtgaaca cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc
     5581 gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc
     5641 tttacggctg gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat
     5701 gattaatatc agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca
     5761 ccacggcaaa catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta
     5821 ttaactttca cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat
     5881 cctaactgtc cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca
     5941 aaaggagctc atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta
     6001 ctattttgcg caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt
     6061 accgctgctg gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacacagct
     6121 cgatgtctgg atcccgactc tcatgagtta cgttccggac tggctacaat ggctgagtta
     6181 tctgttgtgg cctctggctg tcatctctgt gctgttagtg tttggctatt tcttctccac
     6241 gattgctaac tggattgccg ctccgtttaa cggtttattg gctgaacaac tggaagcacg
     6301 attgactggc gctacaccgc cagataccgg gattttcggt atcatgaaag atgtgccgcg
     6361 aatcatgaaa cgcgaatggc aaaaatttgc ctggtatctt ccgcgcgcaa ttgtattact
     6421 aattctttac ttcatccctg gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt
     6481 tagcgcctgg atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc
     6541 gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc
     6601 tttaaccagc ctgtttacga tgatcccgct gcttaatctg ttcatcatgc ccgttgccgt
     6661 ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat aaacacgcga tgtggcggta
     6721 acaatctacc ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc
     6781 ttattccatc ttgcatgtca ttatttccct tctgtatata gatatgctaa atccttactt
     6841 ccgcatattc tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac
     6901 aggccatgag taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc
     6961 gcctgaatcg catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct
     7021 tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc
     7081 tgaaaccagg cgttgaactg gttgaaccga ccagcggtaa taccgggatt gcactggcct
     7141 atgtagctgc cgctcgcggt tacaaactca ccctgaccat gccagaaacc atgagtattg
     7201 aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt gctgacggaa ggtgctaaag
     7261 gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc
     7321 tgctgctgca acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc
     7381 cggagatctg ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg
     7441 gcggtacgct gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta
     7501 tctctgtcgc cgttgagcca accgattctc cagttatcgc ccaggcgctg gcaggtgaag
     7561 agattaaacc tggcccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc
     7621 tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc atttctaccg
     7681 cccgtcgtct aatggaagaa gaaggtattc ttgcaggtat ctcttctgga gcagctgttg
     7741 ccgcggcgtt gaaactacaa gaagatgaaa gctttaccaa caagaatatt gtggttatcc
     7801 taccatcatc gggtgagcgt tatttaagca ccgcattgtt tgccgatctc ttcactgaga
     7861 aagaattgca acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc tttttaggtg
     7921 cttttttgtg gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac
     7981 tggtatttaa ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa
     8041 agctgaatcg attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac
     8101 gtacgaaacg tcagcggtca acacccgcca gcaatggact gtattgcgct cttcgtgcgt
     8161 cgcgtctgtt aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact
     8221 ttagttccac aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac
     8281 cattaccgct ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa
     8341 gggcttcact tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct
     8401 gtttaaactg cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg
     8461 cgaagacgag cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt
     8521 tcccgggttc ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta
     8581 gcatccccgg gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt
     8641 gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt
     8701 cgtgccaagg catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt
     8761 gaagaaaaag aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag
     8821 caggaaatca tagccctgat taaagataaa cacatgacag ctgacgcagc tgctcatgaa
     8881 gttatcgaag gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt
     8941 gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt
     9001 atcgacctga gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc
     9061 gaaaccgcac agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt
     9121 acttcccaca cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt
     9181 agcgtcacct ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag
     9241 gtttacgtca atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg
     9301 gcttctgaaa aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt
     9361 caccaggtag aagtatgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt
     9421 aacggcgctg aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg
     9481 ctgcccactg aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctcg
     9541 caggcggtta tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac
     9601 ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt
     9661 aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg cctcggcttt cggtaaattg
     9721 cgcattatgt tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc
     9781 gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc
     9841 ggcgtaatgg tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt
     9901 gatttcttta gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt
     9961 aatgatatga tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag
    10021 caagttattg atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct
    10081 ggcgatgaac gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc
    10141 gccatttcta tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag
    10201 gtgttagcag agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac
    10261 aagttcattg aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta
    10321 ggagaagatc atgggtttgt tcgataaact gaaatctctg gtttccgacg acaagaagga
    10381 taccggaact attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt
    10441 gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt attgctatca aaccaacggg
    10501 taacaaaatg gtcgcgcctg tagacggtac cattggtaaa atctttgaaa ccaatcacgc
    10561 gttctctatc gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt
    10621 tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg
    10681 cgatactgtc attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac
    10741 tccggttgtt atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt
    10801 aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat
    10861 ggcgcccatc ggcgccattt tttatgcttc cgccagcggc ggcaaaatca attcatcgct
    10921 ctcatgctgc tgggtgtagc gcatcacttc cagtacgcgc aaccccgcac ggtgcaccgc
    10981 atcgtttaac gccttccctt tcagcaaacc actgataagc tgagcacaaa acaggtcgcc
    11041 agtccctttc aggtcggttt ttacccgtga atgggaaatg acattcacgc tgtcggcact
    11101 gaccaccacc acctgcatct cctgattttc ttcattaccg gaggcgctgg taatcaccac
    11161 ccattttaat gtgtctgaaa gcagactttt tgcggcagca atggcactgt cgagatcgcg
    11221 gcaattttta ccggtcaggg tttccagctc aaagatattg ggggtaattc cctgcgccag
    11281 cggcagtaaa tattgtcgat acgcctcggg aaggtcaggt ttgacataaa ttccgctatc
    11341 aatatcgcca atcaccggat cgaccatgat caataggtca ggatggtctt tgcgtagcgc
    11401 agtcagccac tcggcaagga ttttgatttg cgatgccgtt cccatatagc ccgtggttac
    11461 agcacgaagt tggcgcagcg catcacgctc ctgaagcgca cgcaaatagc cgctaaacca
    11521 ttcatccgga atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc tcagcaatac
    11581 cgtcggcacg gcaaagacat tcaggccgtt ctgttttata gcaggcactg caatgctgtt
    11641 gcctacgctg ccgtacacta cctgcgactg cacggcgacg atatccgcct gcagcgccct
    11701 actcttatcg ttaaacaaca acaaactact catttaattt tttctccttg ccgatgatcc
    11761 tcatcatatt ccaaccgaaa ctttacctga ttctggcagt caaatcggct atcacaaaac
    11821 aaggataagg taattcaatg aagaaaatca tttgtctggt cattacacta ctaatgacac
    11881 tccccgttta cgcgaagtta actgcccatg aagaagcccg catcaacgcc atgctggagg
    11941 gattagcaca gaaaaaggat ttgatatttg tacgcaacgg tgatgaacat acctgctatg
    12001 aagcggtttc tcatctgcgt ctgaagctcg gcaatacccg taaccgcatt gacactgccg
    12061 agcagtttat tgataaggtt gcttcgtcgt catcgattac tgggaagccg tatattgtga
    12121 agatccccgg taagagcgat gagaacgcac agcctttttt acatgcgtta attgcgcaga
    12181 cggataaaac ggtgcctgcg gaaggaaatt aatccgcttt gggaaggcat ttacaggagg
    12241 taacatgaaa aaacgcttta tttatcacga tgaaaaatcg aataaatttt ggtggataga
    12301 ttacgaaggg gatagtttag ctgtcaacta tggcaaggta ggtagtattg gtaaattcca
    12361 gacaaaagag ttcgataatg aagaacagtg tctgaaagaa gccagtaaat tgattgccgc
    12421 aaaaatgaag aaaggctatc aagaagatcc aaagtttaac ttcatggatc gctactattt
    12481 tgatgatgaa gaaattgggt tacatgttaa aacgtcacac ccaaacttcc agtgccattt
    12541 tactgatcca ctttatatgt gttgctggga tgaagaatct ccttttggca gcgatgaagg
    12601 tgctgatgct ctaaacgttc ttgaaaatag cctccgtaaa gagccggatc tggactgtgc
    12661 tgatttccct caaatgttaa ttgaaactat gtggggtatg aaatacatcg ctatggacag
    12721 tattcttgaa gaggatgttc gtgcgcaatt actagtcgat gaaatgagca ctatccagag
    12781 caatatgatt acctacgcaa ctgcattcgg tcagattaaa gtcatgggta aaatctccca
    12841 taaacttaaa aagatgggac tcaatgcact agcgcgtcat cagcttaccg caaaaattct
    12901 tcaatggggt gacggtcagg actcaccaat acttcaaaaa atgattgatg accttacggc
    12961 gtttcctcac gaaaattaaa tactgcattt gtcggcagca acaactgtta aaaaagtgcg
    13021 ctttgtttat gccggatgcg gcgtaaacgc cttatccggc ctacaaaatc gtgctaattc
    13081 aatatattgt agaaaccttg taggcctgat aagcgtagcg catcaggcag ttttgcgttt
    13141 gtcatcagtc tccgatgcta ttaatcctta aatccccgcc ccctggctaa agtgctcttc
    13201 cccaaacacc ccggtggaaa ggtagcgatc gccacgatcg cagatgatcg ccaccaccac
    13261 cgcaccaggg ttagcttttg ccacccgcag tgctccggca accgcgccgc cggagctgac
    13321 gccacagaat attccttccc gcaccgccag ttcgcgcatg gtgttttccg catcgcgctg
    13381 atgaatatcc agcacctcat ccaccagcga agcgttgaaa atccccggca gatattccgt
    13441 aggccagcgg cgaatgccgg gaatgctgct gccctcttcc ggttgcaggc cgacaatggt
    13501 caccggtttg gattgttcgc gcataaagcg tgacacgccg gtgatggtgc cggttgttcc
    13561 catgctggag acaaaatggg taatgcgccc gccagtttgc tgccagattt ccggcccggt
    13621 agtggtgtaa tgggcgtaag ggttatcggg attattgaac tgatcgagca gctttccttc
    13681 accacgattc gccatctcca gcgccagatc gcgcgcacct tccatgccct gctctttggt
    13741 gacaagaatc agttccgcac cataagcacg catcgccgca cggcgttcct ggctcatgtt
    13801 gtcgggcatc agcaatttca tgcgatagcc tttaagcgcg gcaatcattg ccagcgcaat
    13861 gccggtgtta ccactggtgg cttcgattaa cacatcaccc ggtttaattt cctcgcgctt
    13921 ttccgcctcg acgatcatcg aaagcgccgc acgatctttc accgaacctg ccgggttatt
    13981 gccttccagt tttaaccaca cttcactgcc gttatccggc cccattcgct gcaacttcac
    14041 cagaggcgta ttgcctattg tttgttctaa tgtactcacg atctctgtcc atacgtggtg
    14101 tcgcctgatg cgacgcttgc gcgtcttatc aggcctacag gttacaaacc ttgccattaa
    14161 aaaacccgga tcgcgggaag cgcctccggg cgtttaacat tcactcaacc tatcaggcgc
    14221 tttgtgcgag agcaagttcc tcatctcggg tttcgatacg ctcgtcgccg ttatacagcc
    14281 gcgcatgttg cagaccaacg aataaacgct cgccacgctg cggggcatcg tcgccatgca
    14341 tcacgaccgt cagcggttcg ttgtaccacc ccagcggctg cacgactaat tgggtgtaat
    14401 gacctttcgg gctggcttcc agtacctgta ccggcagcgg cgaatcgaga ctggtacggc
    14461 ggctgatatc cacttcccac gggcgcagga agagatccac cggcccctga tacgcaggcg
    14521 tataccccag cggccagcga tgtgcgccaa catggaactg cccgccgcga atggttccct
    14581 gcaggcggtt cacttcaccc ataaattcga gcacaaatcg ggtcgctggt tcgcgccata
    14641 cctggtccgg cgcatcagcc tgctcgatat tgccctggct catcaccact acacgatcag
    14701 ctacttcggt cgcttcttcc tggtcgtggg tcacgaacac gctggtgaat ttcagctctt
    14761 catgcagttg acgcagccag cgacgcagct ctttacgcac ctgcgcatcc agcgcgccaa
    14821 acggttcatc aagcaggaga atttgcggtt caacagccag cgcgcgcgcc agcgccacgc
    14881 gctgtttctg gccgccggaa agctgtgccg gataacgatc cgccagatgc gcaagctgga
    14941 ccatctccag caattttgtc actttcgctt tgatggctgc ggcattcggg cgctcgcgac
    15001 gtggcagcac cgtcaggcca aaagcgatat tgtcgaacac cgtcatatga cggaacagcg
    15061 cgtaatgctg gaacacgaaa ccgactttac gatcgcgtgc gtgcaggcgg ctcacgtcgg
    15121 taccgtggaa gcgaatatgc ccgctggttt gatgctccag cccggcgata atgcgcagca
    15181 gcgtggtttt tccggaaccg gacggcccca gcagcgcgac catctgacca gaaggaatat
    15241 ccagtgagat atcgttcagc acctgggtgc gaccaaacga cttcttaata ttggcaatct
    15301 caatgctcat gatgctcctc ctgctgcgcg cgtttttcct gattctccag acgccactgc
    15361 aacatactct ttaaaaacag ggtgataatc gccatcagcg tcaacagcgc ggcggcggta
    15421 aaggagccga cggtgttgta gtcctgctcc agcaattcaa tctgtaacgg cagcgaaagt
    15481 gtttcgccgc gaatcgagcc ggaaaccacc gacaccgcac caaactcgcc aatcgcgcgg
    15541 gcgttggtta gcaccacacc atacagcagc gcccagcgaa tgttcggcaa tgtgacgcga
    15601 cggaacatct gccagccgga tgcgccaagc aaaatcgccg cttcgtcttc ctggctgccc
    15661 tggcttaaca tcaccggcac cagctcgcgc accacaaacg gacacgtcac aaagatggtg
    15721 accagcacca ttccgggcca ggagaacata atttgcagat tatgttcatc caaccagccg
    15781 ccgagcgggc cgttggagcc gtagaacagc agatacacca gaccggcaac caccggtgac
    15841 acggcaaaag gaatgtccag tagcgtcagc agtaactggc gtccggggaa gttaaagcgc
    15901 gtcaccagcc aggccagcag aatgccgaac accaggttca ccggtacggc aatcagcgca
    15961 atcatcaccg tcagccaaat cgcgtgcagc atgtccggat cggccagatt ctgtaaaacc
    16021 ggcatcagcc ctttgctgaa tgcctgcacg aagatgtaaa tcatcggcac cagcaggatg
    16081 aacgccgaaa ccagcatccc gatgccaatt agaaaccatt tgccccagtt aatcgggcgc
    16141 gcgtcataac gcttcaactg tgtcacttcc gccattaatg acctaccaca cgccgaccaa
    16201 agcgactttg cagagtgtta attgagaaca gcagcagcag agatgccgcg aggatcaccg
    16261 aagcaatcgc gctcgccgcc gggtaatcaa actcctgcaa gcgcacaaaa atcatcagcg
    16321 acgtcacttc cgtcttccag gcgatgttcc cggcgataaa tatcaccgcg ccaaactcac
    16381 caagactacg ggtaaacgac aacgccacgc ccgccaccag cgccggagaa agttccggca
    16441 gcaccacttt gcagaaactc tgccagcgcg ttgcgccaag cgtttctgcc gcttcttcat
    16501 attccgggcc taactcttcc agcaccggct gcacggtacg tactacaaac ggaatgctgg
    16561 taaaggccat cgccaccgca atccccagcc atgtataggt gactttgata tcaaacttcg
    16621 ccagccattc accgtaaaaa ccgttcacgg aaaagagcga ggccagcgtt aaaccggcga
    16681 ctgccgttgg cagcgcaaag ggtaaatcca tcagcgcatc aagcagcgtg cggccaggga
    16741 agcgataacg agttaagatc cacgccatca gcagaccgaa aacgccgtta aaaatcgatg
    16801 ccacaaacgc cgacagcagc gtcactttgt aggctgcgac cacctgcggg ttggtgatca
    16861 cctcccagta ctgcgcccag ctcatctggg ccagctgcat caccagcgtg gagagtggca
    16921 ggagcaaaat caggcaaaca aacagcagac tggtgccgag gcttaaggta aagcccggca
    16981 gcacgcgtct tgatgaaact gcaaacatca gttacgcccc gccgctaaca gcttgtctaa
    17041 ctcgccgccg ctggtgaagt gggttttcat cacttccggc caggagccaa atttgtcttc
    17101 cacgcggaac agttcggtct gcgggaattt atctttcagt ttgtccatga cttccgggtt
    17161 atttacgcgg tagtaatagt cggtgatgat ggtctgcgcc tgcgggctgt agagccagtt
    17221 caggtaggct tttgctgctt tttccgtacc gttggcctgc acgtttttat cgacccacgc
    17281 caccgggaat tccgccagaa tgttggtttt cggaatcacc acttcaaagc cctgcgcttc
    17341 atactgttta cggatgttgt tcacttccga ttcaaagcta atcagcacat cgcccagccc
    17401 gcgctcggca aaagtggtgg tcgcgccacg accgccagta tcgaacactt caacgttttt
    17461 caggaactgg gtcataaact gttcggtttt ggctttgtcg ccaccgtcag ctttatccgc
    17521 tgcgccccat gccgccagat aggtgtaacg cgcgttaccc gacgttttcg ggttcgggaa
    17581 aatcagtttc acgtcggagc gcaccaggtc gttccagtcg tggatattct tcgggttgcc
    17641 cttacgcacc aggaagccca tggtggagta gaacggcgag ctattattcg gcaggcgcga
    17701 ctgccagtcc gccgggataa gcttgccttt atcgtgcaga atttgtacgt cggtcacctg
    17761 gttataagtg acaacgtcgg cttttaagcc ctgcaaaatt gccagcgcct gttttgatga
    17821 cccggcatga gattgtttta tcgtcagttt gtcgccgccg ttatctttgg cccattgctg
    17881 ttcaaacggc gggttcaggg cggcaaacag ctcgcgggag acgtcataag aactgttcag
    17941 cagttccgtt gcctgtacat ggcccgccag cagcagagaa gcgaccagcg cgagtgagtt
    18001 ctttttcagt aagttaacgg ccattgcgca cccttataaa tttaatgact ttctaatagc
    18061 catcatattt ataacggacg tgaaaggagt aacggtttta tataccgttt ggtgatttgg
    18121 aagttgaaaa gggaataaga acttggttca acaggcgtaa ttgttgcagt cagtttggac
    18181 acgaacagcg cggagaaacc ggagcgtaca catagtacgt gaggatttcg agcactgccc
    18241 ggggccaaaa tgacaaataa aatagcctgg tgaacttagt tcaagacgaa atcctcccca
    18301 caaatgcagg gaggaaacag aggtgaatca gataccgacg ctaaccgtct ccggcagtgt
    18361 gctgccgcca tcaatcacat tctgtgtacc ggttaaatag ctggattcat ccgatgcgag
    18421 gaaggccgcc agttcgccga cttccagcgg atcggcgagg cgacgcatcg ggattgcttt
    18481 cgccatttca gtcagcaccg actctggatc ttccgggttc gactggcggg caatgctttc
    18541 cgccattggc gtgcgaacgt atcccgggca gatggcgtta acgcgaatac cagactgcgc
    18601 gtactccacc gccagcgatt tagtcaggcc aacaatcgcc gctttcgtta aggcatacgc
    18661 cgtttcaccc ggatcggcca ccatatcgcc agtgactgaa gacatcatca caatgcgacc
    18721 atctttgcgg gcaatcatct ccggcagcac cgccttcgtg acgttccata cgcctttaat
    18781 attgatatca atatggaaat cgcgatcttc atcgctcata tcgaggaaac tgcccagacg
    18841 acaaacgcct gcgttattca ccaggatatc aatgcgccct tctttttcct tcgcgcgctt
    18901 gatagctgcg gcgaccgacg ccgggtcacg cacatcggcg acaaccgccg tacagcgatg
    18961 accacgacca cacagttcgt ccgccagctt ttcgatctca ggggagatat ccagcaagat
    19021 taggtttgcg ccatgacgcg caaaagttct ggcaattcct tcgccaattc cctgcaatgc
    19081 gcccgtaatc agtgctgtct tgcccgtgag tttacccatt ttaatgctct ccttgttaat
    19141 acagagcgtt acactccatt ccctctgaaa atacaggata aacaaaatat tgctgcggat
    19201 cagctcacta attcaggaaa atctcaatcc agtcgctgct ttatgcatca tttcaggcgt
    19261 tgtgttagct cactgctgcg ctgaatcatt ttcaatgact ccacatcatt gagttgcaca
    19321 agaccaacaa acagtaaatc ggtaacagaa ttttgcgccg tgcgggtaga catcgacgag
    19381 ctacgccatt cggtttcacc ggaaacggta tcgagcgtga aatgcgccag ccgccgcagc
    19441 ggagagtcag ccagagaagt aatggcaatc accgttgccc cctgctttcg tgccgcttct
    19501 gcgcacaaga caatctcttt tttactgccg ctgtaagaga tagcgatttg tacatcgcct
    19561 tttttcaacg cctgggaaac ggtggcttgt acgtgcgtgt cggcttcgca ggccacgcga
    19621 tagccaattt tcatcagttt gaacgacaga tcgcgcccca ccagcgccga cccgcccagg
    19681 cccgttatct ggataaacgg tgctttactg atgacctcaa tgattttttg cagccgcgcg
    19741 tagtcgaaca acgcacaggt ttgctccacc gccagctctt tttcgcgatt cagtttgcgg
    19801 gcaatcactt ccagcgaatc atcactggta atcgaactgt gcaggtgcag cgccgtggca
    19861 ttcgttttct cccggctggc actgtattcg ccaattaacg ccatacgcaa ttcggtaaaa
    19921 ccttgcgcgc cgagcttctg ggcaaacttc acaatactcg actggctgat gccgagctgt
    19981 ttcgccatct tgtgagaaga gacagatttc agctcactca cgttggcccg cagaaagtca
    20041 gcaatttttt gctcgttctc ggtaaattca ctttccgcat tgcgtatttt cgtcaggtac
    20101 aacatcttcc actctcctgg gctggcagca taaattccgc ttaagttctt atgacgctct
    20161 tcacactctg cgagtgtaga ctcaatgatt cctttaagtc tgtcaattcg ggatatatga
    20221 ttccatacat ctgcaattat ttgatcgtaa atagtaaggt caccaccgat gcaatttgaa
    20281 aagatgatta ctgaaggctc gaacaccgcc tcggctgaaa ttgaccgcgt atcgacgctg
    20341 gaaatgtgcc ggattatcaa cgatgaagat aaaaccgtac cgcttgccgt tgagcgcgta
    20401 ctgccggata tcgccgcggc gatcgatgtt atccacgccc aggtaagcgg tggcgggcgt
    20461 ctgatttacc tcggtgcggg aacatccggt cgtctgggga ttctggatgc cagcgaatgt
    20521 ccgcccacct acggcgtgaa accaggtctg gtagttggat taattgctgg cggcgaatat
    20581 gccattaagc acgcggtgga aggcgcggaa gatagccggg aaggcggcgt taacgatctg
    20641 aaaaatatta atttaacggc acaggatgtg gtggtcggta ttgccgccag tggtcgtacg
    20701 ccgtatgtga ttgccggact ggaatacgcg cgccagctcg gttgccgtac agtgggaatt
    20761 tcctgtaatc cggggagcgc cgtttcaacc accgcagagt ttgccattac accgattgta
    20821 ggtgccgaag tggttaccgg ttcttcgcgg atgaaagcag gtacagcgca gaaactggta
    20881 ctcaatatgc tctccaccgg gctgatgatt aaatccggca aagtgttcgg caacctgatg
    20941 gtcgatgtgg tcgccaccaa cgaaaaactg catgtgcgcc aggtcaatat cgttaaaaac
    21001 gccaccggat gtaatgccga acaagcggaa gcggcgttaa ttgcctgcga gcgcaactgt
    21061 aaaacggcca ttgtgatggt gctgaaaaat ctcgatgccg cagaagctaa aaaacgcctg
    21121 gatcaacacg gcggctttat tcgtcaggtt ttagacaagg aataacccat ggccaaagag
    21181 atcagcagtg aacttatgaa caccattctt acccgtgtcg gcggaccggg aaatatcgcc
    21241 agttgtggta actgtatgac gcgcctgcgt ctgggtgtac atgacagttc actggttgat
    21301 cccgatatca aaacgctgga aggcgtgaag ggcgtcattt tgaccagcga ccaggtgcag
    21361 gtcgtttttg ggcctggtaa agcgcatcgt gccgcaaaag cgatgagcga gctgctggga
    21421 gaagcaccgg tacaggatgc cgcagaaatc gccgcccaga acaaacgtca gttaaaagcc
    21481 aaacaaacct ccggcgtgca acaatttctc gccaaattcg ccaccatctt cacgccgctg
    21541 atccccggtt ttattgccgc cggtctgctg ctagggatag cgacgttaat tgccacggtg
    21601 atgcacgttc cggcagatgc tcagggcacg ctacccgacg cgctgaattt tatgaaggtg
    21661 ttcagcaaag gtttgttcac cttcctggtg attctggtgg gctataacgc cgcccaggca
    21721 tttggcggca cgggcgtcaa tggcgcaatt attgccgcac tctttttgct cggttataac
    21781 cccgctgcaa ccaccggtta ctacgccggt tttcacgatt tctttggtct gcccatcgat
    21841 ccgcgcggca atattatcgg cgtgttgatt gccgcatggg cctgcgctcg cattgaaggc
    21901 atggtgcgcc gctttatgcc ggacgatctc gacatgctgt tgacctcact aatcactctg
    21961 ctgatcaccg ctacgctggc ctacctgatc attatgccgc tgggtggctg gttattcgaa
    22021 ggcatgtcgt ggctgtttat gcacctgaac agtaatccgc tcggttgtgc ggttttagcc
    22081 ggactgttcc tgatcgccgt ggtgtttggc gtacatcagg gctttattcc tgtttacctc
    22141 gcgttaatgg acagccaggg attcaacagc ttatttccca tcctttcgat ggcaggcgcg
    22201 ggccaggtgg gcgcggcgct ggcactctac tggcgggcgc aaccgcacag tgcattacgc
    22261 agtcaggtac gcggggcgat tattcccggc ctgctgggcg ttggcgaacc gttgatttat
    22321 ggcgtcaccc tgccccgcat gaaaccgttt attaccgcct gtttaggcgg cgcggcgggt
    22381 ggtttgttta tcggcctgat agcctggtgg ggtctgccga tgggcttaaa cagtgctttc
    22441 gggccatccg gcctggtggc gctgccgctg atgacttccg cgcaaggcat tcttccggca
    22501 atggtggttt atgccggtgg gattctggtg gcatgggttt gcgggtttat tttcaccacg
    22561 ctctttggct gccgtaacgt caatctggac tgatatcatg aaacggacaa tgctctatct
    22621 ttctctgctg gctgttagtt gtagcgtcag cgccgcaaaa taccctgttc tgacagaaag
    22681 ttcgccagag aaagcagggt tcaacgtcga acggcttaac cagatggatc gctggattag
    22741 ccagcaagtg gacgccggtt atcccggcgt aaacctgctg attattaaag ataatcagat
    22801 tgtgtatcgc aaagcctggg gagcggcgaa aaagtacgat ggcagcgtgt taatggcgca
    22861 gcctataaaa gctaccaccg ggacgctgta tgacctggcc tcaaacacca aaatgtacgc
    22921 cactaacttc gccctgcaaa agctgatgag cgaaggtaag ctgcatcctg acgatcggat
    22981 tgcgaaatat attccgggat ttgccgatag tccaaatgac accatcaaag gcaaaaacac
    23041 gctgcggatt tctgacctgc tgcatcacag cggcggtttc ccggcggacc cacaatatcc
    23101 gaataaagcg gtcgcgggcg cgttatattc ccaggataaa ggccagacgc tggagatgat
    23161 caagcgcacg ccgctggaat atcagcccgg cagcaaacat atctacagcg atgtcgatta
    23221 tatgctgctt ggatttatcg tcgagtcggt caccggtcag ccgcttgacc gctatgttga
    23281 agagtcgatt tatcgcccgc tcggcctgac acatacggta tttaacccgc tgctgaaagg
    23341 ctttaaaccg cagcaaattg ccgccacgga attgaacggc aatacccgcg atggagtgat
    23401 ccacttcccg aatatccgca cctccacgct ctggggtcag gtgcacgatg aaaaagcctt
    23461 ttattcgatg ggcggcgttt ccgggcacgc aggtttgttt tccaataccg gcgatattgc
    23521 ggtgttaatg caaacgatgc tgaacggcgg gggctatggc gatgtgcagc tgttcagtgc
    23581 ggaaacggtg aagatgttca ccaccagctc taaggaagat gccacttttg gcctcggctg
    23641 gcgcgtgaat ggtaatgcca ccatgacgcc gacgtttggc acgctggcaa gcccgcaaac
    23701 ctacggtcac actggctgga ccggaacggt gaccgttatc gatccggtga atcatatggc
    23761 gattgtgatg ttaagcaaca agccacattc tccagttgcc gatccgcaaa agaatcccaa
    23821 tatgttcgaa agcggtcagc tgccgattgc aacgtatggt tgggtagttg atcaggtgta
    23881 tgcggcgtta aagcaaaagt aataaaaagg ccagtcagta atgttactgc ctggccttta
    23941 atcaatgaat cagaaacgct tacagcgcca tcaacttgtc cagcgacggt gcgaaataat
    24001 agccgccggt taccggtttg gtgaaacgca acatcgcatc acgcttacca tcggtatcgc
    24061 caaacatgct cagcagttgc tgctcaatgt tatgcagacg cgcgcagtag gcgcagaagt
    24121 acagaccgtg agtgccactg gcagtgccgt acggcaggct ctggcgaaca atcttcagcc
    24181 ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga ggtttccgga cgttcgtcgc
    24241 cgtcgatctc ttcgttggcc tctttggtgc gcccgatcat catctcctga tcgtgaacgc
    24301 tcatccggtt gagctgcttc aggttgtgtt cccaacgctg gacaaacaca tagctgccgc
    24361 ccgcatccac gccgtctttg ataaccgcca cttcgcgacg cgtctcttca cccgccgggt
    24421 tttccgtacc gtcaacaaag ccgctcaggt cacgctcttc aacccaacgg aagccgtgga
    24481 tctcttcttt cacttcaatg cagtcaccaa aggcttccat cgccgcctgg gcgacagaga
    24541 agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac atcgaactgg gtggtcggtg
    24601 caaggccttt accgtagccc ggaaaatctt tcagctcttc tgccccaacg ccgccgctca
    24661 gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc aagatgcgcg tccgggaatt
    24721 tcgcttcaaa agttgccagt ttgtcggcaa atgttttact ggccgcacgc agggcgtcaa
    24781 cttccccttt cacgttggct tcgatccaaa tcgccgcgcg gcaatgttct ggcaaaatgc
    24841 cactctgaac ctgagacatt gttcctcctg aaaataataa tgccacgcaa tccgtggcat
    24901 tgttgagcgc tattttacgt gttttttcgc ggcggcggtt tgttcagacg caaattaacg
    24961 ccgccagata attttactga ctttccagtt tttcagggta tcgtccgaag gcattaaccc
    25021 ttcaggacca ctccattccc cgctgaactg gtagctaata tgttgactgc cttcggcttt
    25081 acattcgacg gcacgattat catcaccatc agctttttgg caattgccaa atgctttgct
    25141 gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg tcagcaggaa tatcgctatc
    25201 cagcacgtca atgcgactaa tcgtgccttg atcgccgtta atcaccatcg ccacgttgtc
    25261 gcccttcatc acttcaaaaa agcgcaccac gttgccgttc gcggttttca ttccgctgcg
    25321 caggcgataa tcgccatcaa gcgcatcggc aatggcttgt tcttgcagtg gtgtggacgc
    25381 cgttaattca ccaacgccct gctcgctcac tttggtggac gacccaaacc agttccacgg
    25441 gttagcggca gaccagttaa ctgacgacat cgtggaacag ccggtcagca tcaatggcat
    25501 agcgcataac attaaacgca gcgatttcat gtcacttcct ttggttattc aataacgttg
    25561 cttggagtgc aaattcacca aaaagtgccg ttattttttt acttcattaa aacaagcgcg
    25621 taaacgtcga ttggtcagca gccagattaa cgccacaata tccgccacta ccagcgccag
    25681 accgataccg ctaacggatt caccgttcag ccacagccac ggttgccagc aaagtaaaac
    25741 cacctgcgcc aacagcaaca gaaaatagag tacacgccag gtgcgaggaa acgtagcccg
    25801 ccgaccgctc aacaaaaacg ccagcaccgc cggaatgcca ggaatcagcc ccagccagaa
    25861 attatcgtga tcgggataaa acagatttag cagcgcagta ccctgctcac gcgacgcacc
    25921 ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat agcacaagcc agaagagcaa
    25981 gggtaaacgc aggcgtccgt gcgcatcata atggacagga tggaactcag tactcttcat
    26041 cttcaatcaa acgcttaccc agactcagca cgtcggcgtg ttcatatccc aggcgttcat
    26101 acattccgag caccatgtcg ttatcttccg gcacattgat ctgaattttc gggcagccac
    26161 gagcaatcag ctttttctcc agccgattaa gcaacgcatt ggcaatccca cgcccacgaa
    26221 actctggatg cacgccaaga taataagcag acccgcgatg cccgtcataa ccgcccatca
    26281 ccgttccgac cacttcaccg tttacctcag cgaccagaaa caaactgacg tcatggttca
    26341 tcttacgctc gatgtccatt tccggatcgt tccacggacg cagcaagtcg caacgctccc
    26401 aaagggtgat gacctcttcg aaatcttcct ggcgaaatac gcgtatctcc atggtattcg
    26461 ttaccttttt gcgggttaaa aggctgatta tggcgtgaac ggtcgaatta gccaatatct
    26521 gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt caactattga aatgaaaagt
    26581 aaaacaattc tcaacagcaa acggtcgtaa cggattacgc gatacgatat aacatctgga
    26641 actttattat tacaactcag gccgtatgag cacttttaaa ccactaaaaa cactcacttc
    26701 gcgccgccag gtgctgaaag ccggtttggc tgccctgacg ttgtcaggaa tgtcgcaagc
    26761 catcgccaaa gacgaacctt taaaaaccag caacggacac agcaagccga aagctaaaaa
    26821 atctggcggc aaacgtgtcg ttgttctcga tccaggccac ggcgggattg atactggagc
    26881 gatcgggcgc aacggttcga aagaaaaaca tgtggtgctg gcgattgcta aaaacgtccg
    26941 ttccattttg cgtaatcatg ggattgatgc gcgtttaacg cgttctggcg atacgtttat
    27001 cccactttac gatcgcgttg aaatcgccca taaacatggc gcagatctgt ttatgtcaat
    27061 tcatgccgat ggctttacca acccgaaagc tgccggtgct tcggtatttg ccctctctaa
    27121 ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa cgcgaaaacc gcgccgatga
    27181 agttgccggt aaaaaggcga ctgacaagga tcatctattg cagcaagtgc tgtttgatct
    27241 ggtgcaaacc gatactatta aaaacagcct gacgctcggc tcgcatattc tgaaaaagat
    27301 taagccggtg cataaactgc acagccgcaa caccgaacaa gccgcgtttg tggtgttgaa
    27361 atcgccgtcg gttccttcgg tgctggtgga aacctcgttt atcaccaacc cggaagaaga
    27421 gcgactgtta ggcacggcgg cgtttcgtca gaaaatcgcc acagcgattg ctgaaggcgt
    27481 gatcagttat ttccactggt tcgacaacca gaaagcacat tcgaaaaagc gataagttat
    27541 gaaacccgac gcacaccagg ttaaacagtt tctgctcaac cttcaggata cgatttgtca
    27601 gcagttgacc gccgtcgatg gcacagaatt tgtcgaagat agctggcagc gcgaagctgg
    27661 cggcggcgga cgtagccggg tgttgcgtaa tggtggtgtt ttcgaacagg cgggcgtcaa
    27721 cttttcacat gtccatggtg aggcgatgcc tgcttccgcc accgctcatc gcccggaact
    27781 tgccgggcgc agtttcgagg cgatgggcgt ttcactggta gtgcatccgc ataatccgta
    27841 tgttcccacc agccacgcga atgtgcgttt ttttattgcc gaaaaaccgg gtgcagagcc
    27901 cgtctggtgg tttggcggcg gcttcgactt aacccctttc tatggttttg aagaagacgc
    27961 cattcactgg caccgcaccg cccgtgacct gtgcctgcca tttggtgaag acgtttatcc
    28021 ccgttacaaa aagtggtgcg acgattactt ctacctcaaa catcgcaacg aacagcgcgg
    28081 tattggcggg ctgttctttg atgatctgaa cacgccagat ttcgaccact gttttgcctt
    28141 tatgcaggcg gtaggcaaag gctacaccga cgcttattta ccaattgtag agcgacggaa
    28201 agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc taccgtcgcg gtcgttatgt
    28261 cgagttcaat ctggtctggg atcgcggcac tctgtttggc ctgcaaactg gcgggcgcac
    28321 cgagtcgata ctgatgtcaa tgccgccact ggtacgctgg gagtatgatt atcagccaaa
    28381 aggtggcagc ccagaagcgg cgttaagtga gtttattaag gtcagggatt gggtgtaact
    28441 ccctcacccc cattcccgcg tccgctgatg caacgtcaac gacggcttct cggcaaacag
    28501 ctgctggtaa tccgtggcaa attgccccag atgccagaat ccccactgca tggcggcgtc
    28561 ttttaccgtt gtgctttgcg accacgggct tatcagttcg cggcgtacgg cgttcaggcg
    28621 aatgcgtttc agccacgcgt ttgggccaat gcctaaaata gcgtgaaacg cgttttgtag
    28681 cgtgcggcgg ctgacatgca gttgattaca caaatccagc accgtcaccg gttcggacat
    28741 gttttccagc acatattcac gggcgcggga aagtaatcta cggtaactct gatgactgat
    28801 gctttccgcc gtcaccattg gctgcgcttc ttccagcatc gcccccatcg ccattagcaa
    28861 attatccccc agcacttttc gcactgcagg ctgatggaga ttttccgggt tctcgcaaaa
    28921 cgtcgccagc gcctgttgga caaagcccca cagcgcggct ttatgctgct ctttcacttc
    28981 cagcgccgac tggctacgca acatatgtaa tacccgatcc ggattatgca aaaagttagc
    29041 ctgacgggtg atgacatctt cagaaagcac tacgcccagg atcgtgtaat cgtccggcgt
    29101 acttaattca aactcggttc caccggggcg cgtcgctatt tctgcgcttc ccagacattg
    29161 cgaaccgata aatccctgct caccgcgcgt cgccggaatg ccaaaccaga acgagttcgg
    29221 ccagaccaga cacgactgac gcagcgccag accggtgtat tcacgaaaaa cctgaatatc
    29281 atcgagtaga atttccgtaa attcaccatg aaacttgccc ggatgcagct gatcgtaaat
    29341 ctgctgccag gcggtaatcg tcaaagcatg ttcatagaca tccgttgtct gtcgttgatg
    29401 aacattatcc acttcgacct tcggcgtgag cttcaggttt tcgggtaagg gttcatgata
    29461 aagatggtgc aaattggctg tacgggtctt tttcatgatg ttaatgccgg gtgttgtagg
    29521 acacccggca cctccgacag gttaatgggg cttgagacga taacgactac tgcgtttacg
    29581 taacgtcccg gcagaaaaga gctgttcgag cgcatttctg gctttttcca gcggccagcc
    29641 aaagtgggca gccacttctc ctgccgtcat tccctgacgt actgatgtta acagcgccaa
    29701 caactcgtca gcagattccg cgacgataac tggcgcgtcg ggttccttta cgggttgctt
    29761 cggctggcgg ttaaagccag taaccagcca ctgggtgtca tcgtctggcc gaccgatctc
    29821 cttacggctg atgacacgcc cggtacgcat cgcggcagca caacctgcgt ccagcgcagc
    29881 acgacacgcc gccagatcgc cttccaccac cagcgttaag cgaccagggt caagcacttc
    29941 gtgactgagc agacgaacgt tagctgcttt gagcatggca tccgctgcat ctatcgcggc
    30001 gaccatgccg tccacttcca gcaatcccag tgcattgatc attggcaacc tccgttacgc
    30061 acgctggatt ggattacgcg cgatttccag cactgcatcg gtaaaggcgt tacaggccgc
    30121 tttacacgct gcctggctac cggttaaaaa tgctgccgag tagttggttt cagacggtgg
    30181 cgggacatag gtcaccagct gtacgtcggc agacttcagg gctgcatcaa tgccataggt
    30241 cgcttccagc ggcggtgcca ccagatacgc catcggatcg ccaagcgtga tcccggcggt
    30301 tgatgagaga taagaaccgg tacgcgaaac tacatgtgcc aggaatgccg tatcttgcgc
    30361 gtcattagcc cactggaaag ctgcgccatt ttcaatatgc gcaaccatcg catccagacc
    30421 tgcacgcact tccgccgggt ttggcccacc aagcataatc agcacttcac cggcagtcgg
    30481 tgacgggccg tgtgcagcgc ccgcatacag cgagcggcca tacaccactt ccaccatcgc
    30541 ctgcttggtc gcttcatcag cggcaatgta ggtgacgtca tcggagtcgg cagaaatcag
    30601 accgaggcta cgaatatgcg gcggcaattt cagttcacgc gcaaaatcgg cgttaacgga
    30661 ggcaatcacc cgcatagcgg tgacagacgg tcgaatcaaa tctaaagctg gcatgatgcc
    30721 tccttatcgg gtcatgttga tgccggatgc tttctgctcc agcatacgtt tcgccaaatc
    30781 cacaatgacg gctgcggctt caaccggcgg cgtaccgccc tggtgaatgt tagagatgca
    30841 ggtgcgatcg gcctcaacgg tggtcgccat acgcggcgag taaacggcgt agcaggagag
    30901 gctttctgac tgccctaacc ccggacgttc ccccaccagc aggatcacca ctttcgcgcc
    30961 gaggatctca ccgatctgat cttcaatctt cacgcgacca taacgaacaa agaatggcgt
    31021 gccgactttc agcccggcct gtttcaggcc cgccatcagc ggcggcagga tctcttcgta
    31081 gttcacagtg atggcatcag ttgacaggcc atcagaaata acgacctgta cgtccggatt
    31141 agcaacacac tgtgctttca gcgcttcaac agcttctgca cacaggcggc ggcccatatc
    31201 cgggcgagtc aggtagagat ttttgtcgct aatttcagag cgtacttcca gcaagccctg
    31261 cgctttcacc cactcttccg gcacttcttt aagtacggta tctttcgaac gagagtgatc
    31321 ggccaggaaa cgcagcaacg cctgggtacg cggacgcgga ccggcacgac cggtacaaac
    31381 gcgggccacg gtgctgcgac gcagttctgt taacacatcc gcacgatgtg ggttttcaac
    31441 accaatccac gcttttgctt ccgcagaacc taaatccagc gcgcagcttt ccgaggtcac
    31501 cggtgccgca caggtggtgg tggtgcactt tgcttctgac ggcgcggggg ccgtttgtcc
    31561 cattgacgcc atcacgctgc gtacaatttc ttcaatctgt ttttgatcca tgatgtgtta
    31621 tctccgcgtc atcagaagaa cagtgacgga tcgcccgccc gtttggtcaa gcgaccgttt
    31681 gccataatgc ccatgctttc cagccagcgt tcaaactccg gtgacgggcg caggttgagt
    31741 aactgacgca cagtggcagt gtcgtggaag gcggtggtct ggtagttgag catgatgtca
    31801 tcgcccaacg gcatccccat gatgtagttg cagcctgcgg tggcgagcag gatcatcagg
    31861 ttttcgttga ggttctggtc agcgtcagcg tggttggtgt aacagcagtc acagcccata
    31921 gagatgccgc tcagcttgcc cataaagtga tcttctaagc ccgcacggat aatctggcgg
    31981 tcgttgtaga gatactccgg cccaataaaa ccgaccacgg tgttgacgat aaacggatcg
    32041 taatgacgcg ccagcccata gttacgtgct tccatcgtca cctggtcagc gccgaagtta
    32101 gcgccagcgg atagcgcaga gccttgtccg gtttcgaagt agaggcagtt ttccccggcg
    32161 atacggttga actctgcgcc cactgcgcgc gcttcgtcga gcatcgccag ttccacgcca
    32221 aactctttca gccctttttc gctgccacag atactctgga aaatcagtcc gccaggcgcg
    32281 ccgcgacgaa tcgcttcgat ctgggtggtg acgtgcgcca gtacgcagcc ctgagttggg
    32341 atgttgaatt tgtcgatcac gccatagatg gtgtccagca cgcggcttaa gttttccacg
    32401 tcgtcagtta ccgggttgac gccgatcacc gcatcgccca ccccgaagga aagcccttcg
    32461 tagatttgcg cggcgatact ttgcacgtcg tcacgggtgt catttggctg caaacgggcg
    32521 ctgaaggtgc ccggaatacc gatggtggta ttggcctttt tgattaccgg cattttcttc
    32581 gcgccgtaga tcaggtccgc gttggagcaa atcttcgcta ccgccgcgac cacttccgag
    32641 gtcagccctt tgcgggtaaa ggcaatgtcg tccacgctgg tttcatcgct cagcacatac
    32701 tcacgcagtt cgctgatgct ccagttttta atctggttgt aggccgtttc gttaacatcg
    32761 tcctgaatca gccgcgtcac gcagtcatct tcataggcaa tcaccggatt attgcggatg
    32821 tccgctacgg tcatttccga caacacctgc tttgccgcca cgcgctcctg tgagcttgcc
    32881 gctgcgacgc ccgccagcac atcccccgaa cgcagttcgt tggctttagc cagcacctct
    32941 tttacatcct taaactgata tacattgccg aacaatgtgg tctttagttt cataagtcgt
    33001 tccctcagga agaagtgatg agtgacattt ttcacagcat ccaaacaatt ctatttcatc
    33061 gttagccctt gtcctgtaag gtaattgcct taacacgccc gctattcatc ctgccgaacg
    33121 taaccgaaag cgccggatac atgagcgggg ttatcgagtc gttagatcga ggtatccggt
    33181 tttgagcaag acaccactga cagcaaaggc catagacgcc gcacaaccaa aggacaagca
    33241 atacaaactc accgattcac tgacgcccgg cctgtttttg ctggtgcatc ccaacggatc
    33301 aaagtattgg cgattcaggt tctggatgga caaaaaagag tatttgcagg ctattggagt
    33361 gtacccgcta atcagcctca aggaagcccg taaacgcgct acagagagcc gatctctgat
    33421 tgctaatgga attaatccag tggaagaagc acgcaaggaa aaagccattg atgcgctcaa
    33481 catggcggca ggttttaaga cggtagcgga ggactggctg gctaccaggg tcagcgggtg
    33541 gtcagagtcc tacacgaaac aggttagatc ggcactggaa aaagatgttt accccgtgct
    33601 ggggaagcgg tcaattgtcg atataacggc ccgtgacgtg ctggcaccca aatttggggc
    33661 aatgaaatca cacagatcct gccaggctat tccatccacc acacagaacg ctgagatctg
    33721 atattaccgc cgcaccattc cacgccagac gaatcaaaac aggcttaccc ttccaagtgg
    33781 atagctaaaa attaaggctg tcagaatgcg ttatattgcg tttaaacggc atcgaatttt
    33841 aatgtgcgca atggtgccag attgcaggca aaaaaaatcc cgccggagaa agcggggaag
    33901 tgaaatgaat cgttgtagtt atagatcaca ttttgagtaa gggtaaccag cccttgagta
    33961 atattatccc tgcattacgt gagatgcgca attatttttt gctaattaaa agttttgtaa
    34021 acattacaga atatcccctt tcactatatg gtaaaagatg tgaaacaggg acaggaggaa
    34081 tataatatta agtatcaaca gtaatgttgg cctttaattt cattcagggc gcggtgaggg
    34141 ttttctccat attttcctca ccgctacttt ttgaggtgag aacatgaatg atagcgaatt
    34201 aatgacggtt aacgaggtag cagctttatt cggcgttacc cgtcggacaa tattccggtg
    34261 gatgaacaag attaaaggct ggcccgtccc ggtcagtccg attggctcaa gaattaagtt
    34321 tatccgatct gagattttgg aattctataa aaacaaaggc gctcgccacc aatgaaaaca
    34381 gctactgaga ttatcaaata acctattgat ttaaaaagct agctaccgca atttctgaag
    34441 gaataggcag catgaaaatc aaagcaaaca caattaaaga aataacatca atcaaagtaa
    34501 aaggagcaga tgccatgcat tcattagaca gttattttca gcgcacgaca gcccctaaat
    34561 cagccgccca ggaacgccgt gaggaattcc aggaaaaagt gatgcgttct gctgattata
    34621 ttgcggacaa gttcgtggaa acggttcggc cacttgttga tgaagtagct gataaacttc
    34681 agtctgaaat gccggaagac atggaaggca ctgcaaaggc gcgactcctt tttgaattaa
    34741 gtcgtcgttt cggcgtgtca ataagcactt ttaaataatc ggaggaggaa agacaatgga
    34801 cgcatttgat gtactgacaa acaaaggcga tgaactggta gcacagcgtg ataaaaccgc
    34861 caacgaaggg gaacgcgcac tgttaaataa gcagattaaa gcgatccgta tggcccagtt
    34921 taaattaatc agcaatgaag ttatcgaaat aattaaaccg cagatcccac aaatcgttgc
    34981 tgacgctatt aaagccgccg gactggagaa acgcattgcc gggctgaaaa cgaacgcaga
    35041 gaaaatggag atgcacaaac agagcggtgc tccgactacg gatgagtact tctcaccgga
    35101 tgctgagttt atggcgcagg ttgaggaacg tctgggtagc tgtctcaccg aggaacagcg
    35161 tcgctatttt gatggagtgg atagttcggc aggtattgac ctgaactcgt atttcggtcg
    35221 tgaaattgag cattttgatg ccgccccgtc tatgccggag ccagaaccag aaatggccgc
    35281 cgatcctgaa gctgagcaac ggaaacgcgt attcaatgcg atagggattc ggtactgatc
    35341 ggataaaaga aagccgggaa ttaccccggc tgacttaaca atgtgaccaa tgaacaaatc
    35401 ggacaagaca aacaggataa gcagtctcga aacaatgttc gggaactata ttagcacaag
    35461 tccggagaaa atcatcatgt tggcactacg catcatgcag ggcaccgcta agaccctcgc
    35521 agaacatgtc ttagacctga aacattcgcc actgagcaaa caggccatga agcgccaaac
    35581 gcttcgtctg tgggctgaat attcgcttgg aactatcaat aaaatcatcg acatgaaatc
    35641 ggggccgagc aaccagagcg cggaggaaat ggagttcatc aggcgtttaa ttctcattcg
    35701 cagagacatt catagccagt tacacagcgt gggaatcgat attaatgatg ggacgggcga
    35761 ttaattttcg gacaagtact atgcgcgcgc gaggagctga acatgaagca agatattgca
    35821 gacaggctgg agatactcga aggccagcgg gcagaagcga aacaactacg taagcaggcc
    35881 agaagagcgc acaggaacaa cgaagcagag ttactaacca aatacatcag tttcaccaat
    35941 tactgcatct acgagtgtta caaggaagat gcagaggact ggctggattc attgccagaa
    36001 cagtactgag cagaataaca ccacgtca
//
LOCUS       DAOFQD010000039        35936 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.039, whole genome shotgun sequence.
ACCESSION   DAOFQD010000039 DAOFQD010000000
VERSION     DAOFQD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.039"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(1..496)
                     /locus_tag="QBF46_001966"
                     /pseudo
     CDS             complement(1..496)
                     /locus_tag="QBF46_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(541..1647)
                     /locus_tag="QBF46_001967"
     CDS             complement(541..1647)
                     /locus_tag="QBF46_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017898976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDQ6674154.1"
                     /translation="MSFIYSLAVLGLPTATQLSELENAVTDAVSMFGLRVGQEIGWEV
                     SPDTFIPNQQRSAAVVFFGGLGADHPSLPSLLRSGVPVVPVVSDLCEVATNVPEILRP
                     LNCLSYANGGAKRVATALLECAGLLPKQRRVFVSYRRDEARQAALQMFDALSARHFDV
                     FLDTHSIAPAEDFQTMLWHRLCDSDVLLMLDTPDYFNSRWTAAEFGRALAKGISVLRV
                     GWPGVLCSARLATASLSELGESELDVVSGRISDHAIDRICLQLEEVRSKSHAVRTVNL
                     MSNLRIGLETIGGEVIGMGTGRGIRVRLPDDRMVVVYPTVGVPTSTTMHEASMNSPDE
                     SVAILYDHVGLHPQWLSHLDWLGQYIQVTESPQV"
     gene            complement(1693..2520)
                     /locus_tag="QBF46_001968"
     CDS             complement(1693..2520)
                     /locus_tag="QBF46_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017898975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDQ6674155.1"
                     /translation="MFIDLTVSNIYPTFRFSSDEIVAYKARKGELLISLEKYLLDGRN
                     ILDADKIQKSIFPQHDIDVFISHSHADEDEAIRIALSLENIGLTAFVDSCVWGYADEL
                     LRKIDDKFCIPQGWSHYSYNLRNRTTTNVHLILNAALQQMIKRSELFMFLGTENAINI
                     DDYMSGEGRLSSPWIFSELTFAKSVKRSERRIFQQGNESFEKMKSESRALEPGFSYPL
                     PKLKHEMSLQEFNGWLDKGISVEAGFAGRISGLTHLDTLYSKLGFSEVQLSEPRFKI"
     gene            complement(2593..4209)
                     /locus_tag="QBF46_001969"
     CDS             complement(2593..4209)
                     /locus_tag="QBF46_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017898974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDQ6674156.1"
                     /translation="MATTRRLSGVRVHLSGSNKELRADIDDFVQKIAAKVFSEGGSIV
                     HGSHPSFIAPLRKAAEDFIQTGGRREALTLVRAKSYSTDEYAAEIEEQRTFASVEIVP
                     ADNSDGQAVDGLTPMRDWMADRSDVVICIGGAWWDINKVKAGVPNELDAMLDLGKPGF
                     VVAGFGGAIAGYLEEDPSLLSRLRNGLCHETNATIANSTSVDLVVKLIADQLKNLPLT
                     RRTFSRGRNFRILALDGGGLRGTFTAAVLAKWDDMLKAGGGSNLISHFDLVAGTSTGA
                     ILAIGLAMGLQPREILEFYEKKGPQIFPKDRKLRHWLKSKHDSATLRGLLAEVYGNKT
                     LAEDSRCRLVIPTVRAKEGQAEAIVTPHSPDRTAFRDISAVEAALASSAAPTYFDEVT
                     FDGPVALETFLDGGVWANNPILPALAEAVRYLKIPLERIDVLSIGTLSSESDFTDQLG
                     KGKVGWAPHSVDLFFAAQEHGALVLAQSFLGPTRHMRVNQQTSDEIKMDDAEAIQDMV
                     RRGNEAGKEHFAEVRSRFFDGQHVEVWERF"
     gene            complement(4747..5232)
                     /locus_tag="QBF46_001970"
     CDS             complement(4747..5232)
                     /locus_tag="QBF46_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDQ6674157.1"
                     /translation="MAKVPVFYSFHFDNDVMRVQQIRNIGSLDGNPPTSPNEWEKLKR
                     TGNKAVENWIDQNMKYKRCIIVLIGSETASRPWVEHEIIKAWNDGKALLGIHIHNLKC
                     PRNGISRKGKNPFDLIKFTDGRALSSVVPCYDPSSINTYQDIASNISNWIDYAIRHKA
                     N"
     gene            complement(5244..5771)
                     /locus_tag="QBF46_001971"
     CDS             complement(5244..5771)
                     /locus_tag="QBF46_001971"
                     /inference="COORDINATES: protein motif:HMM:NF025056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDQ6674158.1"
                     /translation="MAIKLKNLRSAASRSGRVFVAKSLNEAVAKKQQTAFLCHSHQDH
                     ELAKGLQVLLKENGWDLYIDWEDSEMPSIPNKETANIIKLKIKTTDWFLFLATKNSTQ
                     SRWCPWEIGFADIAKGYEKILIIPTENDSGVWYGNEYLQLYKRMDEGVNNQTGKSGYA
                     VYDAGINSGTWVENI"
     gene            complement(6720..7169)
                     /locus_tag="QBF46_001972"
     CDS             complement(6720..7169)
                     /locus_tag="QBF46_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HDQ6674159.1"
                     /translation="MALRVFSPIISSSLQQRGWDVLGLGSRDGIALIRKINDGLEGSV
                     VKRISEWAKITPTELQKMSGIPITTFKRSTKKRFTANQSERLVRVIRIIERAVALFEG
                     DKEAAKKWLHEPNRALNWNIPAEIVASETGAHEVIRLIVRIEDGVYL"
     gene            complement(7340..8767)
                     /locus_tag="QBF46_001973"
     CDS             complement(7340..8767)
                     /locus_tag="QBF46_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004722605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ6674160.1"
                     /translation="MRREKAEYHQAILPEHRGNPLIEALKPKLGINELLEQFSNYPVL
                     DEHIRQHPDPLVREEYTIRLKQLRQPFLIYYECFRAIETAIKDGYSTKNPFSPTTAQY
                     LHYPVDARPAIEPRTGYFEPKAENITLIGASGVGKTSMLEQILNYLPNVIEHDFYHGH
                     RMEFTEQVVWIKVECPNNSSVRDLCEEILVALDLAMGREKSISANTIGSLMRQIEQRI
                     KSSFLGILVIDEMQRLVFKRTGGENNLLNFLHSLVNKLGIPLFFCGNPPFDDTLSKTL
                     KAARRAESSGYFRMESLKKESDEWAAFIEELWEMQWTCIYNELTDELNNKMYELSLGN
                     LDIAHRIYREAQRLVILADSDDERINTSVLEQAYVRACGLSAGTDEIKELRSKMSLPK
                     GHRGQKIRRPQCIPDNPREFIADITRAQHPEFELSLRELVGAVNLSERIGDPDIFQRA
                     AKQKYPVDLLRQVGIIDDDPLGRLS"
     gene            complement(8771..10882)
                     /locus_tag="QBF46_001974"
     CDS             complement(8771..10882)
                     /locus_tag="QBF46_001974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6674161.1"
                     /translation="MAIDINSVWDLNDVEGLKDGPYRIIDIIPHLDCIILFPLTNNHR
                     LRRPTAILLSSFENYMASGSVMLIDFQIPSFLLLAEENLPSSFKEKRDANFSLISDLV
                     SERDFLFTYATSKRMTSLVQYAQEKGTDRKKLSRLLNAFWQYGQTKNALLPAYSNSGG
                     AGKTRKAKKGTLGAPKISRTLSIQRADKYMLTVIDKQNIRKSLMANYLRVNGKSLAES
                     YKEYLRKYHEDELKMASISNRAPSVPSLRQFSYWKERLFSDDIVIRKRSTERDYLLNK
                     RSVLGYAAQDDLVPGSCFEIDATVADVHIVSSFGEQYILGRPTIYSIVDRATLMVVGL
                     HVSLYYASWRAARQALVNCFSPKSEYCAQYGISIDESEWPCAHVPRRFICDNGEMIGL
                     KPQEKVVPFTELSFAPSYRPDYKSFVERRFKILNDSLIHQLQGSTKGGRVVRGDRDPR
                     KDSKHTLYEVTSMLIDAALEHNRTIQKSLAFTRPLLIEKSLSPTPLNTWKIYISQHRH
                     SLSRVAPDEVIARLLPPEQVSMTRSGIKYKEMFYSCAQIEDESLAAIARVHGRWKMDA
                     RVDDNTVNHIYVRLDEKQPFTKCHLSSRNVMLKDIPVSEAEFIQDWLGSHLEKMPVTV
                     ESIDMKKRHVQFEKQAKKRTNTHSVPYREKIKNIRENRLEELRRTTHSFPEHGEDNTA
                     EIKLEAKVVAMLPRRNRKPGK"
     gene            complement(10872..11693)
                     /locus_tag="QBF46_001975"
     CDS             complement(10872..11693)
                     /locus_tag="QBF46_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004722610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 transposase TnsA N-terminal
                     domain-containing protein"
                     /protein_id="HDQ6674162.1"
                     /translation="MATGRRLKTLDDFNRSLKNKYGVGVGTEYKPWLRIQDVASRGIR
                     SQIWGRKTNRVHHLLSSIESQFFYLAEFSDSVTDIREQFPLLPITLSNKIAVTLGVEH
                     PKVPVNGELNIMTTDFLLTCQKDGRKFYHAVSVKPENGIDQRTAEKLDIERVWWELLG
                     VKYSCFMGNELTKIQSDNIRWATAPFRSGMTVYDSSIVQRVLTAVEPGKYLLSDLCKD
                     LTLMCDREREDGLNLLRYLIADKFINVNLDYSIEACGGVEIQSVSSDKRLVSNGY"
     gene            complement(11836..13029)
                     /locus_tag="QBF46_001976"
     CDS             complement(11836..13029)
                     /locus_tag="QBF46_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDQ6674163.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAV"
     gene            13261..14760
                     /gene="pitA"
                     /locus_tag="QBF46_001977"
     CDS             13261..14760
                     /gene="pitA"
                     /locus_tag="QBF46_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDQ6674164.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(15004..15339)
                     /gene="uspB"
                     /locus_tag="QBF46_001978"
     CDS             complement(15004..15339)
                     /gene="uspB"
                     /locus_tag="QBF46_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDQ6674165.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            15730..16164
                     /gene="uspA"
                     /locus_tag="QBF46_001979"
     CDS             15730..16164
                     /gene="uspA"
                     /locus_tag="QBF46_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDQ6674166.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            16481..17950
                     /gene="dtpB"
                     /locus_tag="QBF46_001980"
     CDS             16481..17950
                     /gene="dtpB"
                     /locus_tag="QBF46_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDQ6674167.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(17999..18751)
                     /gene="rsmJ"
                     /locus_tag="QBF46_001981"
     CDS             complement(17999..18751)
                     /gene="rsmJ"
                     /locus_tag="QBF46_001981"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDQ6674168.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAIVTKGHRFDIYAGTPV"
     gene            complement(18759..20801)
                     /gene="prlC"
                     /locus_tag="QBF46_001982"
     CDS             complement(18759..20801)
                     /gene="prlC"
                     /locus_tag="QBF46_001982"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDQ6674169.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            21004..21846
                     /gene="rlmJ"
                     /locus_tag="QBF46_001983"
     CDS             21004..21846
                     /gene="rlmJ"
                     /locus_tag="QBF46_001983"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDQ6674170.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            21918..23273
                     /gene="gorA"
                     /locus_tag="QBF46_001984"
     CDS             21918..23273
                     /gene="gorA"
                     /locus_tag="QBF46_001984"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDQ6674171.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMT
                     R"
     gene            23696..24985
                     /locus_tag="QBF46_001985"
     CDS             23696..24985
                     /locus_tag="QBF46_001985"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6674172.1"
                     /translation="MARLITTTPVSIGGKISFSGAVIHFNEQRTLPPLPALYDAAGYY
                     VNSVNLWFISLVSERMLQDLSSCSRALLRYWRFLERTDIQWDFMPFSRADKPLYLFRN
                     TDLLPAINNGDLAYSTASTYMGHIIQFYNWAVSNNLLKTDEKHAPFGVDIIHIQNPDE
                     PGKFQRRSMTIPYDLRIRSPRKTNINGAASLNPLSLEELSLMFHQLPSTSVEFRLICT
                     LALQSGLRLEEACGFTVDALFQAAPTSHLKNRYLIRIGPENGIPTKYSKTRKIEISAS
                     LLESLKEYVIQERRLRRLNTLLNRRSVAPETKNTRCRFEPLFISQQGNPVTPAVFATR
                     WAEFRTHLKTFVPTFRHKFHDLRCTYATLRLQHLLDAGLAPHEAMDLLMGWMGHNNEA
                     TTWKYLTYLRYETLLKDKFEMMDNIMHLTLKEPPGDK"
     gene            25146..26672
                     /locus_tag="QBF46_001986"
     CDS             25146..26672
                     /locus_tag="QBF46_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674173.1"
                     /translation="MISALKQRNDLSSATKGTLFTFMISHTRLCDRAQLTPFSAEAVQ
                     LQAQFFTDQNRKGKLKDTSLVGLRSQLSSCLNLLGLPAEALMSDVPVFSRLQRVPVQG
                     YSTTELRRLLPLLRGMFKQLSGQFIADPAKHINAYRTTPSMHFNWQGVIYPVCGGISK
                     LMCCALYLLAYYTWSNTSVLLSLKRPHNSARSVSESWYRMPAFKRRAFRTVTVYFTGH
                     DHLDIPKYSLQFFDQLTAVSRLLSDEPETFLLKTAVNGKIVPFPITALSTFNRRFISG
                     VLGIKDDSGAPLHLTAARFRATGSMLSLTRYDPLQTSVLLDNTPAVVNTHYASGNKHD
                     NNRMLRDVTAVLEYQSRHRSGIEAAEDELRKQLDIPVLTYKAYLESVSRPLRSAHGCY
                     CSAPEGTESRKFTLQSLTHQLVGNGELLACASLLNCFACRHQVLVESETDIWCLLSFR
                     DTLRNSREHHLNLNHYQKNFLTLLSSLDERIKLLNTDMVRRAERRLKNHGPHPLWTTE
                     "
     gene            26697..28820
                     /locus_tag="QBF46_001987"
     CDS             26697..28820
                     /locus_tag="QBF46_001987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674174.1"
                     /translation="MAYKVPPLAIEIPEGSSQAFIPGTSDIIALVKSGNRNALNRTVI
                     CYDNDGHPVALFGQDTWNCRPYAHTKESRVSKNRLRFAYLNSCPDLQLEAKIIVYGWL
                     FHRSSTRSRPAKFSSIESRCNTPLNEIFSFLAENKHDSIVALNQTGVWQSFLAWVQAK
                     DWSHISVGHVLRTLRFIAKNACWHNIRLILPEFKVDDLAGRLCHNERRTHQQILALPQ
                     SVADTLYGEAIRFVESAWPHRLALEETERRLQENYLQAKSIVDAYLAEGHFPALVNPE
                     GEITDKHRYSLTIKSFLRERVQDTIHSCLGPSGLLPGEKADGDWLKKFRGQLLTSCFI
                     CCAAFSGMRLSELFELTKDSFHTEVIGGETFFLLKGRTFKFGEKQETWVTSPITEKAI
                     QLATSLTRLARCQLEDAAKWSTGISEKEHYLNLSRSLWLSQTYRSRLPVLIIRWNARL
                     QAFSRQVSATIDNDTLRECITLNPNSRLRTEKLQNSNTNWPMTTHMFRRTLAVFAVKN
                     NLGSAIAIKQQFGHLRLKMSEWYCEGGIASRMNNVLIDTELQDLIDRERLDNNTRLYY
                     DWFNGITPLSGTHGKAIVAMRQDLPVVYRSWESIWQLLSTGRLTLHGTLHGYCKNGYN
                     CSMDGVVNPAFCVNCRGGGSIIDAENAAWWQRKHAQLIVWLTENPTFSPQEYAHCITQ
                     IRAAENVMQDFDLSFQCYEGKGEQR"
     gene            28817..29251
                     /locus_tag="QBF46_001988"
     CDS             28817..29251
                     /locus_tag="QBF46_001988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674175.1"
                     /translation="MKGSDNAQLRLGSALTRLLEGTPMHIQADRKLSVRAVEEEARLG
                     NGSAYYYPELITRIKVAILQGRKCGPAAPSPIPTLRARLNKTQKLKLALREENRALKL
                     TVSNMAAEHHRLAWALREAHLRIEELEVALTEAKRAGIHRLK"
     gene            complement(29309..29562)
                     /locus_tag="QBF46_001989"
                     /pseudo
     CDS             complement(29309..29562)
                     /locus_tag="QBF46_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060488.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(30174..31562)
                     /locus_tag="QBF46_001990"
     CDS             complement(30174..31562)
                     /locus_tag="QBF46_001990"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDQ6674176.1"
                     /translation="MLREWQKECSEAALEKYQSGSPHFLCLASPGAGKTVMAAEVARK
                     MLELKLVDLILCFAPSSVVVSSIESTFSHILQRNFNGGLGAVGAVYTYHSLMYFDKSF
                     WHSLQMHRVLVVFDEIHHCSGDSLNNANMWGGEILNRVQQCTSYTLALTGTPWRTDNN
                     PIVLSNYTDPQGKICCDYVYGLHEAIVDGVCRKPKIALINSNNLLYSSGEIVQHFDSI
                     AGFLSETITSYQSIIWHPDAMKYLLKSGCKKLCEIRKVNSDAGGLVVASSVEHAYQLL
                     NILENEFAQTATIVTYHDRDALVKIENYRQSTTEWIVSVGMISEGTDIPRLQVCCHLS
                     SVKTELYFRQVLGRILRVNQSENQEAWLFTIATDELTLFSNRLAEDLPEDYKILQKQS
                     DEWSLSIHETESTSPEIVRRNGMSKIGEFNLKMNFSEITISPPAVLDKTKQLNMGSLY
                     QQVIDAFLFSVI"
     gene            complement(31556..32125)
                     /locus_tag="QBF46_001991"
     CDS             complement(31556..32125)
                     /locus_tag="QBF46_001991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674177.1"
                     /translation="MKSAVKLHPFIYNLLIKKDFKEFGAIHLRDALLKITDSYTDVTE
                     ARKFIHRQLLRLEALGFISKLENSDGRPKNLYKKTESFYSTTFIPRKLPSNSRLAAMP
                     ENVKLSVLDVDLFLMDLRKEKTVHEAKLAVILSEIEEYQSLMERFPSRRNCFIELHQH
                     AKNQSAALLGRVTALSKVLGQLSNGDCAC"
     gene            32225..32536
                     /locus_tag="QBF46_001992"
     CDS             32225..32536
                     /locus_tag="QBF46_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6674178.1"
                     /translation="MAHPNPIPDRLKAARTKAGITQKELGIRIGMEPSSASGRMNHYE
                     KGRHTPDIDTLKRMADELGVPISYFFCENETTAELARLIANMSEQQQVELIKILKKDQ
                     R"
     gene            32967..34037
                     /gene="capV"
                     /locus_tag="QBF46_001993"
     CDS             32967..34037
                     /gene="capV"
                     /locus_tag="QBF46_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cGAMP-activated phospholipase CapV"
                     /protein_id="HDQ6674179.1"
                     /translation="MSDISAGNHYGVRILSLNGGGARGMFTISVLSEIERILASKHPN
                     QDIKIGDYFDLITGTSIGGILALGLATGKSARELERVFFDRANNIFPKRWSLTNLLRS
                     LCAPIYRSNPLRETIQEMIGAETTFNDLTRRVMIPAVNLSTGKPQFFKTPHNPDFTRD
                     GPLKLIDAALATSAAPTYFAPHYCEDLRAYFADGGLVANNPSYIGLLEVFRDMNSDFS
                     VTHKDIHILNIGTVGEEYSLSPKLLSKTWWNGYCRLWGMGKRLVLTTMTANQHLHKNM
                     LQRELMLHDAVDNYIYLDEIIPNEAASDITLDNATKSSLENLSARGRQLANVKFAQDQ
                     KLKNFFTTPAKPFKRMNAQEIQ"
     gene            34037..35338
                     /locus_tag="QBF46_001994"
     CDS             34037..35338
                     /locus_tag="QBF46_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674180.1"
                     /translation="MRWDFNNYYSHSVDGLISKLKLGKSESDKLKALRQKVRERTRDV
                     FKEARQIATDARKQTLTLESVRFKLEQTNVRYLSPSDRETLARLIFEMEDVARDDFIK
                     FQPRFWTQGSFQYDTLNRPFHPGQEMDIDDGTYMPMTVFESEPSIGHTLLLLLVDTSL
                     KSLEAENDGWVYEEKNTCGRIKIPHEKTHIDVPMYAIPKEQFQTKQTAADSAHIIKSA
                     DAMFESLSANMDTREAYLVEPDKVNLALRQGARRWSISDPKIVEDWFNDSCKRIGGHL
                     RSICRFMKAWRDAQWEVGGPSSISLMAAVVNILDREAHNGSDLTGTMKLVAGLLPNEF
                     CSGLESPDDTDEKLLFPAECDHNVHQRTIVETMRNLEGILLDAESSNSREEALRRIND
                     AFGKRVTNASLIISIAAAPAFQISPSKEQAPVKINTTMVSG"
     gene            35338..>35936
                     /locus_tag="QBF46_001995"
     CDS             35338..>35936
                     /locus_tag="QBF46_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674181.1"
                     /translation="MEEGRLHKMMIECGYRYTLARKLPEKAFLHSQERLKGYYTKEYE
                     TEAGNFNVALVIRPDPFTELPLACIVDLPEQFRDRLMPHISQEGILCYVEQLEADWDP
                     NNLEGTYKEVDAQIHRTLINSVLAATTGLNDKRELEGEFATYWRPSDSLYILSKASRG
                     IRLKTLFSESTFPDGSPRLEYITVQESSPEDSETILKKWL"
BASE COUNT         9613 a         8901 c         7998 g         9424 t
ORIGIN      
        1 ctgtcacgca gatccagatg gattttgcga taaccataga cgcatcccga ttccagccag
       61 aactgtttaa tctgtcctgt cagtctcagg tctgcctgat ggcgttgtga atgcggctgc
      121 tgaagccagg cgtaaaaacc actgggatga acatccagca cccgacagag caggcgaaca
      181 ggccagcaac aggtgttgtc acggataaag gcgtacctca gtcggacagc tttgcgaagt
      241 acgccgcggc tttttttaat atgtcccgtt cgtcggtaac ccgcttcagc tctttctgga
      301 gacggcggat ctcggcctga gcatctgact gttctttatt agtggaagaa tccggaccgt
      361 acttctttat ccaggagtaa aggctgtggg tggtgatatc gagacgtgtt gcaacgctgg
      421 caacagaata accgcgatca acaacctgtt tgactgcttc aattttaaac tcttcgggat
      481 aacgcttacc gctcatgggc acctctcttt aagtcatctt aaatgactct gaggtgtctg
      541 ttaaacctgt ggcgattcag tgacttggat gtattgaccc agccaatcca agtgactaag
      601 ccactgagga tgcaggccta catgatcata gagtatggct acggattcat caggagaatt
      661 cattgatgct tcgtgcatag tcgtggaggt cggaacgccc accgttggat aaaccacaac
      721 catgcggtca tcgggcagac gcactcgaat ccctcggccg gtgcccatgc caatcacttc
      781 tccaccgata gtctcaaggc ctattcggag attgctcatt aggttaacgg tacgcaccgc
      841 atggctcttg ctgcggactt cttcaagctg cagacagatc cggtcaatcg cgtggtcact
      901 aatacgtccc gatacgacat ctaactcact ctcaccaagc tcagaaaggc ttgcagtcgc
      961 taaccgcgcc gagcacagaa ctcccggcca tcctactcga agaacactga tacccttagc
     1021 caaggccctt ccgaactccg ctgcagtcca tcgactgtta aagtagtcgg gagtgtcgag
     1081 catcaacaac acgtcagaat cacaaagccg atgccataac atggtctgaa aatcttctgc
     1141 cggggcaata ctatgtgtat ccaggaaaac gtcaaaatgc ctagcagaga gggcatcgaa
     1201 catctgcagt gccgcctgac gagcctcgtc tcgccgataa cttacaaaaa cccgacgttg
     1261 cttcggaagc aggcctgcac actccaaaag tgcagtggct accctttttg ccccaccatt
     1321 ggcgtaggac aaacaattca atggacgcag gatctccggt acattggtcg ccacttcgca
     1381 taaatcagac acgacgggaa caacaggcac accgctacgc agcaagctag gtaagctggg
     1441 atgatcagca cctaatccgc cgaaaaatac aacagcagca gatcgctgtt gattagggat
     1501 aaaagtatca ggactaactt cccagccgat ctcctgaccc accctcaatc caaacatgga
     1561 aacagcatca gtgacagcgt tttcaagctc agagagttga gtagcggtcg gtaagcccaa
     1621 aacagccaaa ctatatatga atgacatcca tacctcgcag aaaccaacta aatcctcaca
     1681 aaacagaaat acctaaatct taaatcttgg ctcactcagt tgaacttcac tgaaccccag
     1741 cttgctatac aacgtatcca agtgcgtcaa cccgcttatt cgcccggcaa aaccagcctc
     1801 gactgatatt cctttatcta gccaaccatt aaactcctgt aggctcattt catgtttcag
     1861 cttcggcagg ggataactga agcccggctc aagagctcta ctttcgctct tcattttctc
     1921 gaaactctca ttaccctgtt ggaaaattct acgttccgaa cgcttgacac ttttagcaaa
     1981 tgtaagctct gaaaaaatcc atggagatga cagacgcccc tctccagaca tgtagtcatc
     2041 gatatttatt gcgttttcag tgcccaaaaa catgaacaac tctgatcgct ttatcatttg
     2101 ctgtaaagcc gcattaagga tcaaatgcac gttggtcgtt gttctgttac gtagattgta
     2161 actgtaatgg ctccatccct gtggtataca gaatttatca tcgattttcc gcaaaagttc
     2221 gtcagcatac ccccaaacac atgagtcaac gaaagccgtg agcccaatat tctccaggct
     2281 gagggctatc ctaatggctt cgtcttcatc agcatgagaa tgagagataa aaacatcaat
     2341 atcgtgctgc ggaaagatcg atttctgtat tttgtcagca tcaagtatat tacgtccatc
     2401 aagaaggtat ttttcgaggc tgatcaacag ttcccctttt ctggccttgt acgccactat
     2461 ttcgtcagaa ctaaaacgaa atgttgggta gatattacta acagttagat cgataaacat
     2521 taattccccc cagtcaagac ctacgtgcca agcggatgtc taaacgggta acgctagggc
     2581 atccgcgctt aattaaaatc gctcccacac ctcaacgtgt tgtccgtcaa agaatctgga
     2641 gcgcacctca gcaaagtgtt ctttgccagc ctcattgccg cgtcggacca tgtcttggat
     2701 agcttcagca tcatccatct taatttcatc actggtctgt tggttaacac gcatatgacg
     2761 tgttggaccc aagaaacttt gagcgagtac cagagcacca tgctcttggg ctgcaaaaaa
     2821 gagatctaca ctgtgaggtg cccagcctac tttgcccttt ccgagttggt cagtgaaatc
     2881 actttcgcta ctgagtgtgc cgatgctcag cacatcaatc ctttctaggg gaatcttcag
     2941 gtatcgaacc gcttccgcca acgccgggag gatggggtta tttgcccaga cgccaccgtc
     3001 caggaacgtc tccaacgcaa caggcccatc gaaggtaact tcatcaaaat aggtaggtgc
     3061 agcggaagaa gctagggcgg cctcaacggc tgaaatatct ctgaatgcag tccggtcggg
     3121 gctatgtggg gtaacaatcg cttcagcttg accttccttg gccctcaccg tagggatcac
     3181 caagcggcag cgagaatctt cagccagcgt tttgttcccg tacacctcgg ccaaaagtcc
     3241 acggagagtt gctgagtcat gcttggattt aagccagtgc cgaagtttcc gatccttcgg
     3301 aaaaatctgg ggaccctttt tctcgtagaa ctcaaggatt tcacgtggct gcaaacccat
     3361 cgccagtcca atagccaaaa tagcacctgt ggatgtacca gctaccaaat cgaaatgaga
     3421 aattaaatta ctcccgccac cggccttcaa catatcgtcc cacttggcca aaactgcagc
     3481 agtgaaggtg cctctgagcc cccctccgtc gagcgccaaa atacggaaat tacggccacg
     3541 cgagaaagta cgccgagtaa gtggcaggtt cttcaactga tcagcaatca gcttcacaac
     3601 cagatcaacc gatgtactgt tggcaatagt cgcattcgtt tcgtgacata gtccgtttcg
     3661 caaacgagat agcaatgaag gatcttcttc gagatatcct gcgatagcgc cgccaaagcc
     3721 cgcaacaacg aagcctggct ttccaagatc aagcattgca tctagctcat tggggacgcc
     3781 tgcttttact ttatttatgt cccaccatgc accaccgatg cagatgacta catcggaacg
     3841 atcagccatc caatcacgca ttggggtcaa gccgtctaca gcctggccat cgctgttatc
     3901 tgcaggcaca atttctacag aagcaaatgt tctctgctct tcaatttcag cagcgtactc
     3961 gtcagttgag taactcttcg cccgcacaag tgtaagcgct tcccttctac caccggtttg
     4021 gatgaaatct tcagcagctt tacggagtgg cgcaatgaac gaaggatggc ttccatgaac
     4081 aattgagcct ccttcactga aaactttagc ggcaattttc tgcacaaaat catctatgtc
     4141 tgctcgcagt tctttattcg acccggaaag atgaactctg actccggata gacgccttgt
     4201 ggttgccatg tgtatcacct caattacctg aaatgtcatt ttctgccagc aagtacgtct
     4261 tggggacacg ttttcaacga ccggctaaca tagttgtggt ttcaatcaag cctgcgatat
     4321 tttctcggcc attttgaacc gcgaattcgt atctcttgca gtacttggat agcttaacaa
     4381 ttgtgttact agtatgatct tcaaaataca gatcagaggc agggatgact aaggtgtagc
     4441 caaagtgttc caccctttta cgatcctttc gcccgctaaa taaatctttc cgttgattag
     4501 gcaggctcac actttatcca gtggatgtgt aaattttatt cgatgctctt agtgttaatt
     4561 attttgtctt atagatcttc cgttagaagc ctattatctt tcaacatgct gcgaaaaatt
     4621 tcgtaaaatg cccattgcct agcagtggtg aactgatgtg caaggacata agttacagag
     4681 gttttcgtgg cttatgactc gtcaatttta catcattaat tcatttcgaa aaaatgaaat
     4741 aaaaaatcaa tttgccttat gtcgaatggc gtaatcaatc cagtttgaaa tattgcttgc
     4801 tatgtcctga taagtattga tagagctagg gtcgtaacaa ggaacaacac ttgacagtgc
     4861 tctaccatca gtgaacttaa tcaggtcaaa ggggtttttc ccttttctac taatgccatt
     4921 tctagggcat tttaggttat gaatgtggat gcctaaaaga gcttttccgt cattccatgc
     4981 cttgataatt tcatgttcaa cccatggtct tgaagcagtt tcgctaccaa tcaaaacaat
     5041 aatacatcgt ttgtatttca tattttgatc tatccagttc tcaactgctt tattaccagt
     5101 acgtttgagc ttttcccatt catttgggga ggttggaggg ttaccgtcaa ggctaccgat
     5161 gttacgtatt tgttgaactc tcattacatc gttatcaaaa tgaaagctgt aaaaaacagg
     5221 aactttagcc attttagttt cctttaaata ttttccaccc aagtgccgga atttattcca
     5281 gcgtcataca ccgcatatcc agacttaccc gtttgattgt taactccttc atccatcctt
     5341 ttatatagct gaaggtattc gttgccatac caaacacctg agtcattttc tgtcggaata
     5401 atcaatatct tttcgtagcc tttggctatg tctgcaaaac caatttccca aggacaccaa
     5461 cgtgattgag ttgaattttt tgttgctaaa aacaagaacc aatcagtagt ctttattttt
     5521 aacttaatta tgtttgcagt ttctttgttg ggaatacttg gcatttcgct gtcttcccag
     5581 tcaatgtata agtcccatcc attctccttc aataaaactt gaaggccctt agctaattca
     5641 tggtcttgat ggctatggca taaaaaagca gtttgctgtt ttttggccac agcttcatta
     5701 agcgatttag caacaaagac tcttccggaa cgactagctg cagatcgtaa attttttagt
     5761 ttaatcgcca ttatgatcct gtaataggta ataatccgaa ccagtaatag tttatacttt
     5821 gcaacattat aaaaaataga tcaactgctc aggcaaagtt aggttattag atgttcttat
     5881 caaactttgg agcataaaag cttgagacaa gtcaatgtat ctatgagtca gtaaaaaaaa
     5941 ttagagaaag ttatacaggt ttctcagttt ttcaacagta cgaacgtgtt tagagctcca
     6001 aggaacaaag cccttttcgc taacctccct gacatacgat ctgaactcga aatgctctat
     6061 atgaaatcaa gagagaaagg ctccacactt atgggacaga ttacagcaat aaacatgttc
     6121 tatctaaatg aattggctcc gttggttttt aaaactgaaa gaatccctaa tgcctcgccg
     6181 acgggttatt gtgatagagg ggatacccct ccactgaaac tccttaagcg taatttgatc
     6241 ttagccagga aagaaaacta agactgggca gtagcatttt tctgcccatg agatgttgca
     6301 tgcggattga attgttactc ctaaaagaag ctatacctaa ttggtctatc catattaatc
     6361 aaaaaaaaat tccaacgctt catacttcag tttggttaca aaaaggctgt aaatcaattt
     6421 tttggctgcg aaatggggaa attaagtggc tttgaaatac attagttgat cacaaataat
     6481 ttttgtaaat aggtcattct cgcattgagt gcaatgcaga actaaaatca tctagaaagg
     6541 tatcttaatt tttaggacaa gaactcatca tgatttattt tttgactctt ggatatccaa
     6601 ccacctcgaa ctattaaaat aatttaaaaa cgtatattaa atatattaac atcaacacca
     6661 tttaatattt ctaaaaaaat aagctatcaa aatttgtatt ccatgatgtt atcatagaat
     6721 cataaataaa caccatcctc aattctaaca ataaggcgaa taacttcatg ggccccagtt
     6781 tcagaagcta caatttcagc tggaatattc caattcagag cacgatttgg ctcatgtagc
     6841 catttttttg cagcctcctt gtcaccctcg aacaatgcga ccgcccgttc gattatccgt
     6901 ataacacgca ccaatcgctc actctgatta gcagtaaatc gttttttggt agaacgttta
     6961 aacgtagtga ttggaatacc cgacattttt tgtagttccg tcggcgtgat tttcgcccat
     7021 tcagaaatac gttttacaac actcccttca agcccgtcgt tgatttttct tatcaaagca
     7081 ataccatccc gggaacccag tccaaggaca tcccaccccc tctgctggag agagctactt
     7141 ataatagggg aaaagactct taaagccata cgtcgcttcc ccagttgata gttccgctac
     7201 aatgcatgaa gattctccta taatacacta aatatctctc accttatcta tcagtacggt
     7261 ttaacattgt ttgcccctga cggttaagaa aaaaatattt tggtcactta caactgttac
     7321 ttataagata ttaatctact caagacagtc ggcccaaagg atcatcatca ataatcccta
     7381 cctgtctaag cagatcaaca ggatactttt gcttagcggc ccgttggaaa atatctggat
     7441 caccgatcct ttccgaaagg ttaaccgcgc caaccagctc acgtaaactc agctcgaact
     7501 cgggatgctg agcccgagta atatctgcaa taaattcccg aggattatcg ggtatacact
     7561 gtggtctacg tattttttgc cctcgatgcc cttttggtaa agacattttt gatcgtaact
     7621 ctttgatttc atcggtacca gcagacaaac cacaagctct tacatatgcc tgttccagta
     7681 cactagtatt aattctttca tcatctgaat cagccaagat aacaagtctc tgcgcctccc
     7741 gataaatacg gtgagcaata tccaaattac cgagagataa ctcatacatt ttgttattca
     7801 actcatccgt tagctcgtta tatatacaag tccattgcat ttcccaaagt tcctcaataa
     7861 atgctgccca ctcatcgctc tcttttttta aggattccat tcggaagtat ccagaacttt
     7921 cagcacggcg ggctgcctta agagtttttg atagcgtgtc atcgaatggt ggattgccgc
     7981 agaaaaagag ggggattcct aatttattta ctaggctgtg aaggaagtta agaaggttat
     8041 tttcacctcc cgtgcgcttg aacaccaacc tttgcatttc atcaataact aagatcccca
     8101 aaaaacttga tttaattcgc tgttctattt gtctcatcag actaccgatt gtatttgcag
     8161 atattgattt ttctctaccc atagctaaat ctaatgctac caagatctcc tcacagagat
     8221 cacgaacact ggaattatta ggacattcca ccttaatcca gacaacctgt tctgtgaact
     8281 ccattctatg cccatgatag aaatcatgtt ctatcacatt tggtagataa ttcagtatct
     8341 gctcgagcat actggtttta cctactccac tagcacctat caatgtgatg ttttctgctt
     8401 taggctcaaa ataacctgtt ctcggttcaa tcgcaggtct ggcatcaact ggatagtgaa
     8461 gatactgagc agtagttggg ctaaagggat tttttgtcga ataaccgtct ttaattgcag
     8521 tttctatcgc tctaaaacat tcataataaa taagaaaagg ctgacgcaat tgtttcaggc
     8581 gtattgtgta ttcttctcta actaaaggat cggggtgctg tcggatatgt tcatccaaaa
     8641 ctggataatt tgaaaactgc tcaagaagct catttatacc taatttaggt ttcagtgctt
     8701 caatcagagg gtttcctctg tgctctggta aaattgcctg gtgatattct gctttttctc
     8761 ttcgcatcag ctattttcct ggcttacgat ttcgccttgg cagcatagct accacctttg
     8821 cttccagctt aatctcagca gtattgtctt caccatgctc agggaaagaa tgtgttgttc
     8881 tcctgagctc ctcaaggcga ttttcccgaa tgttctttat tttttccctg tatggcacag
     8941 agtgagtatt tgtcctcttt ttagcctgct tttcaaattg aacatgccgt ttcttcatgt
     9001 ctatcgattc tactgtaaca ggcattttct ctaaatgtga ccccagccag tcctggataa
     9061 actcagcttc actcacgggt atgtctttaa gcattacatt ccgggatgat agatgacatt
     9121 tcgtaaacgg ttgcttctca tctaatctca cataaatatg attcaccgta ttatcatcaa
     9181 cccgggcatc catcttccag cgcccatgta ccctcgcaat agcagcaagg ctttcatctt
     9241 caatctgtgc gcaagaataa aacatttctt tgtatttgat accacttcgg gtcatgctga
     9301 cctgttctgg tggtaacaat cgagcaatca cttcatcagg agcaactcga ctaagtgaat
     9361 gcctgtgctg agaaatatat atcttccacg tattgagcgg agtaggactt aaactttttt
     9421 caatcaaaag aggtcgggtg aaggcaaggg atttctgaat ggttctgtta tgctctagcg
     9481 cggcatcaat cagcattgaa gtcacttcat acaatgtatg ttttgagtcc ttacgcggat
     9541 ctctatcccc gcgtacaact ctcccacctt tcgtcgatcc ctgcagttgg tgaatcaaac
     9601 tatcatttaa aattttgaaa cgacgttcaa cgaaactttt ataatcaggt ctataagaag
     9661 gagcaaaact cagttcagta aaaggtacaa ctttttcctg tggttttagg ccaatcattt
     9721 caccattatc acagatgaag cgcctaggaa catgcgcaca aggccactca gactcatcaa
     9781 tagaaatccc atactgagca cagtattcag atttaggtga gaaacaatta accaatgcct
     9841 ggcgcgcagc acgccaagag gcataatata aggagacatg cagtccaact accataagcg
     9901 tcgcccgatc gacaatagag taaatagttg gccgtccgag aatatattgc tcgccaaaac
     9961 tggatacgat atgtacatca gctactgttg catcaatttc aaaacagcta ccaggaacta
    10021 aatcatcctg cgcggcataa ccaagcacac tacgtttatt tagtaagtag tcacgctcag
    10081 ttgaacgctt gcgaatgact atatcatctg aaaataagcg ttctttccag tatgaaaact
    10141 gacggagtga aggtacacta ggtgctctat tactgatact tgccattttc aattcatctt
    10201 catggtattt acgaagatac tccttatagg attcggcaag agatttacca ttgaccctta
    10261 aataattcgc catcagtgat ttacgaatat tctgtttatc tatcacggta agcatatatt
    10321 tatctgcacg ttgaatactg agtgttcttg atatttttgg agcaccaagt gttcctttct
    10381 ttgccttccg agtcttccca gcccctccac tatttgagta ggcaggtaat aatgcatttt
    10441 tcgtctgacc atattgccaa aaagcattca gtagtcgaga taacttcttc cgatcagtac
    10501 ctttctcttg agcatattga actaaactgg tcatcctttt tgaagtagcg taagtaaaca
    10561 aaaaatcacg ttctgaaacc aaatcactaa tcaaactaaa attagcatct ctcttttctt
    10621 taaaagaaga tggaagattc tcctctgcaa gcaaaagaaa gcttggaatt tgaaaatcaa
    10681 tcagcataac actgcctgaa gccatgtaat tttcaaaaga tgatagtaat attgcagtgg
    10741 gtcttcgcaa gcggtgattg ttcgttaagg ggaacaagat aatacagtcc aaatgaggaa
    10801 taatatcgat gatacggtaa ggtccatctt tcagaccttc tacatcattc aaatcccata
    10861 cactatttat atcaatagcc attagaaacc agccttttat cactgctaac agactgaatt
    10921 tcaactcctc cacaagcctc aatcgaataa tctaaattaa catttataaa tttatctgcg
    10981 ataagatagc gcagcaaatt tagaccatcc tcacgctctc tatcacacat gagggtaaga
    11041 tctttacata aatcagataa taaatatttg cccggttcaa ccgctgttaa aacccgctga
    11101 actatagaag aatcgtatac agtcatacca gatctgaacg gtgctgttgc ccacctaata
    11161 ttgtcgctct ggatctttgt tagctcattt cccataaagc acgaatactt gacacccagc
    11221 aattcccacc atacacgctc aatatccagt ttctcagcag ttctctggtc aatgccattc
    11281 tctggtttta cagaaacagc atgatagaac ttacggccgt ctttctggca cgtcagcagg
    11341 aaatccgtcg tcataatgtt gagctcccca tttacaggga cctttgggtg ctcaaccccc
    11401 aaagttaccg ctatcttgtt tgacagagta atagggagca gtggaaattg ttccctgata
    11461 tcggttactg aatcactaaa ttcagctagg taaaaaaact ggctctcaat cgaagacaac
    11521 agatgatgta cacgattggt tttgcgcccc caaatttggg agcgaatgcc cctagaggcc
    11581 acatcttgaa tacggagcca aggtttatac tcagtaccta cacctacccc gtacttattt
    11641 tttaacgacc gattaaagtc atctaaggtt tttaaccttc taccggtagc cattgagacg
    11701 acccatatat accccgatgt ttaagtatag aatgcagctc gcttatgggc aaaattctct
    11761 tacgggcaaa ctttattgta tttttacgct cttaagggca atctttattg tctgcgggca
    11821 aactttattg ttagttcaca cagcaatcaa atcctgcgca caagcccatg cactcgacca
    11881 cgcccactgg aagttatagc cccccagcca gccggtgacg tccatcactt cgccgatgaa
    11941 gtacagccca ggcactttgc gcgcttccat cgtccgtgaa gagagttcgt tggtgtccac
    12001 gccgccgagc gtcacttcgg cagtgcgata gccttcagtg ccgttgggtt gtacgcgcca
    12061 gtcggtcaat gtgctaatca gtgcctgttg gtcacgcacg ttgagctgtt ttagcgaaac
    12121 atccgggatt tgcccgagtt gctgtaagcg ttcaaccaac cgcttcggta gatgaaccgc
    12181 cagtgtgttt ttcaggctct gattcggatg tgcgttacgc tgctcattca ggaaggtttc
    12241 gaggtccaca tcaggtagca gattgatact gacaaattca cccggttgcc agtagcttga
    12301 aatctgcaac accgccggtc cagacaagcc gcgatgggtg aagagtaagt tctcacggaa
    12361 aacgatgccg ttttcagcgg taatcacgga aggcaccgcc acgcccgcca gcacctgtaa
    12421 ctcttcgagc aacggtttat gcagagtgaa tggcaccaga cccgcacggg tcggcagcac
    12481 gttgaggcca aattgttcgg caatcttata accaaacggc gacgcgccca gccccggcat
    12541 tgacagccca ccggtcgcga tgaccagctt ttcgcaaccg acagtcatgc cgttcagttc
    12601 aagcgtgaag cctgtttcat ccttcgccac actcagcact tcgctacgca atctgaaggt
    12661 tacattgccc ttctcgcact catccaccag catgtcgaca atctgctgcg cggagtcatc
    12721 gcagaagagt tgtcctaacg ttttctcgtg ccaggcgatg ccgtgtttat tgaccagatc
    12781 aatgaaatcc cactgggtaa aacgcgcgag tgcagactta caaaaatgcg gattctggct
    12841 cagataagcg cctggttcga cataaaggtt ggtaaagttg cagcgcccac cgccagacat
    12901 aaggattttg cgccctggtt ttttaccatt atcgatcagc agaacccggc gtcctgcctg
    12961 acctgccagc gcagaacaga acatacccgc cgcaccagcg cctataataa tggcatcaaa
    13021 cctttccacg ttgcgctcct cttagaaaaa acgggcgtga attgtaaaga ttcctcagtg
    13081 gtcgcaccag catcaatatt actaaaagga agtatttgcc tgaattatat aagataatta
    13141 ttttttgagt gaaatccata caggaggcaa atcaaaaaaa gtctatattt cactttgccc
    13201 gcgccgcgaa agtcactgat aatgcgccgc gttcatgtcc tcaaaatggc gtaacgtcct
    13261 atgctacatt tgtttgctgg cctggatttg cataccgggc tgttattatt gcttgcactg
    13321 gcttttgtgc tgttctacga agccatcaat ggtttccatg acacagccaa cgccgtggca
    13381 accgttatct atacccgcgc gatgcgttct cagctcgccg tggttatggc ggcggtgttc
    13441 aactttttgg gtgttttgct gggtggtctg agtgttgcct atgccattgt gcatatgctg
    13501 ccgacggatc tgctgcttaa tatgggatcg tctcatggcc ttgccatggt gttctctatg
    13561 ttgctggcgg cgattatctg gaacctgggt acctggtact ttggtttacc tgcatccagc
    13621 tctcatacgc tgattggcgc gatcatcggg attggtttaa ccaatgcgtt gatgaccggg
    13681 acgtcagtgg tggatgcact caatatcccg aaagtattaa gtattttcgg ttctctgatc
    13741 gtttccccta ttgtcggcct ggtgtttgct ggcggtctga ttttcttgct gcgtcgctac
    13801 tggagcggca ccaagaaacg cgcccgtatc cacctgaccc cagcggagcg tgaaaagaaa
    13861 gacggcaaga aaaagccgcc attctggacg cgtatcgcgc tgatcctttc cgctatcggc
    13921 gtggcgtttt cgcacggcgc gaacgatggt cagaaaggca ttggtctggt tatgttggta
    13981 ttgattggcg tcgcgccagc aggcttcgtg gtgaacatga atgccactgg ctacgaaatc
    14041 acccgtaccc gtgatgccat caacaacgtc gaagcttact ttgagcagca tcctgcgctg
    14101 ctcaaacagg ctaccggtgc tgatcagtta gtaccggctc cggaagctgg cgcaacgcaa
    14161 cctgcggagt tccactgcca tccgtcgaat accattaacg cgctcaaccg cctgaaaggt
    14221 atgttaacta ccgatgtgga aagctacgac aagctgtcgc ttgatcaacg tagccagatg
    14281 cgccgcatta tgctgtgcgt ttctgacact atcgacaaag tagtgaagat gcctggcgtg
    14341 agtgctgacg atcagcgcct gttgaagaaa ctgaagtccg acatgcttag caccatcgag
    14401 tatgcaccgg tgtggatcat catggcggtc gcgctggcgt taggtatcgg tacgatgatt
    14461 ggctggcgtc gtgtggcaac gactatcggt gagaaaatcg gtaagaaagg catgacctac
    14521 gctcagggga tgtctgccca gatgacggcg gcagtgtcca tcggcctggc gagttatacc
    14581 gggatgccgg tttccaccac tcacgtactc tcctcttctg tcgcggggac gatggtggta
    14641 gatggtggcg gcttacagcg taaaaccgta accagtattc tgatggcctg ggtgtttacc
    14701 cttccggctg cggtactgct ttccggcggg ctgtactggc tctccttgca gttcctgtaa
    14761 tcgtacgcac caaaacgagc gggtcagctg gcccgcttca gattgtgaca tagtgcgctt
    14821 tgttcatgcc ggatgcggcg tgaactcctt atccggccta caaaaacatg caaattcaat
    14881 agattgcaga gattatgtag gcctgataag cgtagcgcat caggcaattt tgcgtttgta
    14941 atcagtctcg agcgggtcag tttactggcc cgcttttttt atgcgttatt attgctgaag
    15001 tgcttaatgc caaatcatca atgcaatcag gctgaccacc accagaccac acaatgcgct
    15061 agtcagaata aactgccgac gcacccgctc acagcggcga ataaactcat catcgtgatg
    15121 atcgcgataa cgttgggcat agatatacca aacgagacgc acctgtttgt tgggttggcc
    15181 atgtgaggta aaaaagcccc ctccatcaac atattgatag agcaatggat cgcagttacg
    15241 cagtaccact aacaacgcgc gtagtgatga gaaatagcgc gccatgttaa caatgcaaac
    15301 gacacataaa gcccaaaata atgcgacggt gcttatcata cctcctcccc ggcgacctgc
    15361 ccgcggagtt ccaccccggg gctaccgctc ccgatacgct gccaatcagt taacaccagg
    15421 tcctggagaa accgcttttg tggtgaccaa catccgagcg gctctataga tagtgtagga
    15481 gatcaggttg ttttttttcc agaaggttaa ccactatcaa tatattcatg tcgaaaattt
    15541 gtttatctaa cgggtaagca aggcggattg acggatcatc cgggtcgcta taaggtaagg
    15601 atggtcttaa cactgaatct ttacggctgg gttagccccg cgcacgtagt tcgcaggacg
    15661 cgggtgacgt aacggcacaa gaaacgctag ctggccagtc atcgacaact ttatggaagg
    15721 agtaacacta tggcttataa acacattctc atcgcggtcg atctctcccc ggaaagcaaa
    15781 gttctggtag agaaagcagt ctctatggct cgcccctaca atgcgaaagt ttctctgatc
    15841 cacgtagatg taaactactc tgacctatac accgggctta ttgatgtgaa tctgggtgat
    15901 atgcagaaac gcatctctga agagacacat catgcgctga ccgagctttc cactaatgca
    15961 ggctacccaa tcactgaaac cctgagcggc agcggcgacc tgggccaggt tctggtcgat
    16021 gcaatcaaga aatacgatat ggatctggtg gtttgtggtc accaccagga cttctggagc
    16081 aaactgatgt cttccgcacg tcagctgatc aacaccgttc acgttgatat gctgattgtt
    16141 ccgctgcgcg acgaagaaga ataatcttcc ctccacgacg tgttcctgaa cgcccgcata
    16201 tgcgggcgtt ttgctttttg gcgcgccttg ttacctgatc agcgtaaacg ccttatctgg
    16261 cctacgatct gcgtacgcaa tcaaaatctc ctgccaatac aacatttaac accatcatat
    16321 tttccatcat tagtgtgatc agctggttat tttctgttgt aatagtgtat taatctattc
    16381 accgcatcaa tattaagaat ctctgacaga tgtaaacttt ttcgcgcgtt atcccttacg
    16441 cgttcatact tttcaggatg gtattggaag gttaataaat atgaatacaa caacacccat
    16501 ggggatgctg cagcaacctc gcccattttt catgatcttt tttgtcgagt tatgggagcg
    16561 attcggctac tacggcgtgc agggcgtact ggcggttttc ttcgttaaac agcttggatt
    16621 ctcgcaagag caggcttttg tcacttttgg tgcttttgct gcactggtct atggcctcat
    16681 ttccattggc ggctatgtcg gcgaccacct gttggggacc aaacgcacca ttgttcttgg
    16741 agcacttgtg ctggcgattg gctacttcat gaccggcatg tcgctactta agcctgacct
    16801 gattttcatc gccctgggga ctatcgctgt cggtaacggc ctgtttaaag ctaacccagc
    16861 cagcttgctt tcgaagtgct atccgccgaa agatccgcgg cttgatggcg cattcaccct
    16921 gttctatatg tcgatcaata tcggctcgtt gatagcgtta tcgctggccc ctgtgatcgc
    16981 tgatagattc ggttattcag tcacctacaa cctgtgcggg gcggggttaa ttatcgcatt
    17041 actggtttac atcgcctgtc gtggaatggt gaaagacatt ggttctgaac ccgacttcaa
    17101 gccgatgagc ttcagcaaac tgttgtacgt attacttggc agcgtggtga tgatcttcgt
    17161 atgtgcatgg ctgatgcaca acgtagaagt cgccaatcta gtgctgattg ttctctccat
    17221 cgtcgtcacc atcatcttct ttcgtcaggc attcaagctg gataaaaccg ggcgcaataa
    17281 aatgtttgtc gcctttgtcc tgatgctcga agcggtggtg ttttacattc tctacgccca
    17341 gatgccaaca tcgctgaact tctttgccat caacaacgtg catcatgaaa ttctcggttt
    17401 ttccatcaac ccggtcagct tccaggcgct taacccgttc tgggtggtac tcgccagccc
    17461 aatactggca ggcatttaca cgcatctggg taacaaaggc aaagacctct cgatgccgat
    17521 gaaatttact ctcggcatgt ttatgtgctc actgggcttt ttgacggcgg cagctgcggg
    17581 aatgtggttt gcggatgcac aagggctgac atcgccatgg tttatcgtgc tggtgtactt
    17641 attccagagc ctgggtgaac tgtttattag cgcccttggc ctggcgatga ttgctgccct
    17701 ggtgccgcag catttgatgg gctttattct cgggatgtgg ttcctgacgc aggctgccgc
    17761 gttcttgctg ggcggctatg tggcaacatt taccgcggtg ccggacaaca ttaccgatcc
    17821 gcttgagacg ttgcccgtct ataccaacgt gtttggtaag attggtctgg tcacgctggg
    17881 cgttgcagta gtgatgctgt tgatggtgcc gtggctgaaa cgcatgattg cgacgccgga
    17941 aagccattaa ttattcttgc agaaagcagg gtagcgttat cgctaccctg ctttactttt
    18001 acactggcgt acctgcatag atatcaaagc gatgcccttt agtcacaatc gcgtttggcg
    18061 tggcgacatt cgccagcggt ggcgcgtagt ccggacgctt taccaccacg cgtttggtcg
    18121 ccagcaagcg tgcaggctcc agtaatccat cggcatcaag atccggtccc accagcgact
    18181 gaaagacacg catctctttt ttcactagcg cgcttttctg cttatgcggg aacatcgggt
    18241 cgagataaac cacctgcggg cgcggggtaa tatcagtcag cgccgtcagg ctggaggcgt
    18301 gaattaactg caaccgctcc tgcaaccagc cgccgatttc cgcatccgca taaccacgcg
    18361 ccaggccgtc gtcgagcaag gcggcaacga ctggattacg ctccagcatc cgcacgcggc
    18421 aaccgactga agccagcaca aaggcatcgc gccccagccc tgcggtggcg tccaccacat
    18481 ccggcaaata atcgccttta atgcccaccg ctttcgccac cgcctcaccg cgaccaccgc
    18541 cgaatttacg tcggtgcgcc atcgctccgc caacaaaatc aacaaagatg ccgccaagtt
    18601 ttggctcatc acgcttgcgc aattccagat gttccggcgt taacaccagc gccatcaggt
    18661 tgtcttcatc gtgctccagc ccccagcggg ccgccagaac agataaggca ccgtctccgg
    18721 tgcctgtttc atcaattaag cagattttca ctgaatgatc agcccttaat gccgtaatgc
    18781 tccagcatcg catccagctg cggttcacga ccacggaagc gtttgaacag atccatcggc
    18841 tcttctgaac cgccacggct cagaatgttg tcgaggaacg actgcccggt ttcacggttg
    18901 aaaatgccct cttcctcaaa gcgcgagaaa gcatctgccg ccagtacgtc agcccacagg
    18961 tagctgtagt aacctgcggc ataaccaccg gcgaaaatat ggctgaaagc gtgcgggaaa
    19021 cggccccagg atggagacgg tactacggca accagtttct tgatttctgc cagagtttcg
    19081 aggattttcg ctccctgatc cgggcggaac tcggcatgga ggcggaaatc gaacaggccg
    19141 aactccagct ggcgcagaat aaacagcgcc gcctggtagt tcttcgccgc cagcatttta
    19201 tccagcaact ctttcggcag cggttcgccg gtttcgtagt gaccagagat aaacgccagc
    19261 gcctcaggct cccagcacca gttttccata aactgactcg gcagttcgac cgcatcccac
    19321 ggcaccccac tgatcccgga tacaccggcg gtttcgatgc gggtcagcat atgatgcaga
    19381 ccatgaccga actcgtggaa cagggtgatc acttcgtcat gggtaaacag cgccggttta
    19441 ccatttaccg ggcggttaaa gttacaggtc agatacgcga ccggtttttg cagagaaccg
    19501 tcagctttgc gcatctggcc tacgcagtca tccatccacg ccccgccgcg cttgttttca
    19561 cgggcataca ggtcgaggta gaagctaccg cgcagttcgt tgttctcgtc atacagttcg
    19621 aagaaacgta catccgggtg ccagacatca acatctttac gctctttagc ggtgatgccg
    19681 taaatacgtt tcaccacttc aaacaaaccg ttaaccgctt tgttttccgg gaagtacgga
    19741 cgcagctgtt cgtcactgat gctgtagagg tgctgttttt gtttttcgct gtagtacgcg
    19801 atatcccacg gctgcaactc atcgacgcca aattcggctt tggcaaaggc gcgcagttgt
    19861 gccagctctt tttcgccttg cggacgcgcg cgttttgcca gatcggttaa gaaatccagc
    19921 acctgctgcg ggttttctgc cattttagtg gcaagtgatt tgaaggcgta gttttcaaag
    19981 cccagcagtt gcgccagttc gtgacgcagc gcgaggatct cttccatcac cttgctgttg
    20041 tcccacttac cggcgttcgg gccttgatcg gaggcgcggg tgctgtaagc gcgatacatc
    20101 tcttcacgca gagcctggtt gtcgcagtag gtcattaccg gcaggtagct tgggatatcc
    20161 agcgtcagca ggtagccttc aagttctttc gcttcagcct gggcttttgc cgcagccagc
    20221 gcgctttctg gcatccccgc cagttccgct tcgtcggtaa cgagtttggt ccagcccatt
    20281 gtcgcatcga ggacgttgtt gctgtactgg ttgcccagct cggaaagacg agtggcaatc
    20341 tcaccgtagc gttgttgttt ctctattggc agaccgatgc cagagagttc gaagtcgcgc
    20401 agtgcgttat caaccgcttt tttctgagcc gtgttcagcg tggcgtaatg atcgccatcg
    20461 cgcaggtcgc gatacgcttt atacagccct tcatgttgcc ctacccaggt gctgtattcc
    20521 gacagcagcg gcagggtttg ttcgtacgct tcacgcagtt ccgggctatt tttcaccgag
    20581 ttcaggtggc tgaccgggga gaagatacgc cccagcacat cgtccacttc cgccaacggc
    20641 tggcagagat tttcccaggt gtacggtgct ccttgcgcta ctacgcgctc cacattttcg
    20701 cggcagtcgt tcagcgcctt agtcacggct ggaacgacat gttccgggag aattttagaa
    20761 aacggaggca attcaaaggg agtcagtaac ggattcgtca ttagcgcagt cctggttaaa
    20821 gaggttaagg aagcgctcaa caggcgcttt acataatgtg tgtagcatgg ggttaagtgt
    20881 agtgaatttc aatgagaaac gttacgcttt cgcggcggcg gcctcttttc ggtatactgt
    20941 cctgataggc ttttgtgcgc cccgaatacg ggccgatttt tacttaccgg aacaccttta
    21001 cccatgctca gttatcgcca cagctttcac gctggcaacc acgccgacgt ccttaaacat
    21061 accgttcaga gcctgatcat cgagtcgctg aaagagaaag ataaaccgtt tctctatctc
    21121 gacacccacg caggggccgg gcgttatcag ttaggtagcg aacatgccga gcgtaccggc
    21181 gaatatctcg aaggtatcgc ccgtatctgg cagcaggacg atttgcccgc agaactggag
    21241 gcgtacatca atgtggtaaa acacttcaac cgtagcggcc agttgcgtta ctaccccggt
    21301 tcgccgttga ttgctcgcca gctactgcgc gaacaagaca gcctgcaact gaccgaactg
    21361 cacccgagcg attacccgtt gctgcgttct gaatttcaga aagacagccg tgcgcgcgtc
    21421 gaaaaagccg atggtttcca gcagcttaag gccaaactgc cgccggtttc ccgccgtggt
    21481 ttaatcctta tcgacccgcc gtatgaaatg aaaaccgact atcaggcggt ggtcagcggg
    21541 atagcagaag gttacaaacg tttcgccact ggtacttacg cactgtggta tccggtggtg
    21601 ctgcgtcagc aaattaagcg catgatccac gatctggaag cgaccggtat tcgcaaaatt
    21661 ctgcaaattg aactggcggt actgccagac agcgatcgcc gtggcatgac cgcttccggc
    21721 atgattgtga ttaacccgcc gtggaaactg gaacaacaga tgaataacgt gctgccgtgg
    21781 ctgcacagca aactggttcc ggcaggcacc gggcacgcca ccgtaagctg gatcgtgccg
    21841 gagtaattgc agccattgct ggcacctatt acgtctcgcg ctacaatcgc ggtaatcaac
    21901 gataaggaca ctttgtcatg actaaacact atgattacat cgccatcggc ggcggcagcg
    21961 gcggtatcgc ctccatcaac cgcgcggcta tgtacggcca gaaatgtgcg ctgattgaag
    22021 ccaaagagct gggcggcacc tgcgtaaatg ttggctgtgt gccgaaaaaa gtgatgtggc
    22081 acgcggcgca aatccgtgaa gcgatccata tgtacggccc ggattatggt tttgatacca
    22141 ctatcaataa attcaactgg gaaacgttga tcgccagccg taccgcctat atcgaccgta
    22201 ttcatacttc ctatgaaaac gtgctcggta aaaataacgt tgatgtaatc aaaggctttg
    22261 cccgcttcgt tgatgccaaa acgctggagg taaacggcga aaccatcacg gccgatcata
    22321 ttctgatcgc cacaggcggt cgtccgagcc acccggatat tccgggcgtg gaatacggta
    22381 ttgattctga tggcttcttc gcccttcctg ctttgccaga gcgcgtggcg gttgttggcg
    22441 cgggttacat cgccgttgag ctggcgggcg tgattaacgg cctcggcgcg aaaacgcatc
    22501 tgtttgtgcg taaacatgcg ccgctgcgca gcttcgaccc gatgatttcc gaaacgctgg
    22561 tcgaagtaat gaacgccgaa ggcccgcagc tgcacaccaa cgccatcccg aaagcggtag
    22621 tgaaaaatgc cgatggtagc ctgacgctgg agctggaaga tggtcgcagt gaaacggtgg
    22681 attgcctgat ttgggcgatt ggtcgcgagc ctgccaatga caacatcaac ctggaagccg
    22741 ctggcgttaa aactaacgaa aaaggctata tcgtcgtcga taaatatcaa aacaccaata
    22801 ttgaaggtat ttacgcggtg ggcgataaca cgggtgcagt ggagctgaca ccggtggcag
    22861 ttgcagcggg tcgccgtctc tctgaacgcc tgtttaataa caagccggat gagcatctgg
    22921 attacagcaa cattccgacc gtggtcttca gccatccgcc gattggtact gttggtttaa
    22981 cggaaccgca ggcgcgcgag cagtatggcg acgatcaggt gaaagtgtat aaatcctctt
    23041 tcaccgcgat gtataccgcc gtcaccactc accgccagcc gtgccgcatg aagctggtgt
    23101 gcgttggatc ggaagagaag attgtcggta ttcacggcat tggctttggt atggacgaaa
    23161 tgttgcaggg cttcgcggtg gcgctgaaga tgggggcaac caaaaaagac ttcgacaata
    23221 ccgtcgccat tcacccaacg gcggcagaag agttcgttac catgacacgt taacgtagaa
    23281 aaatcactgc tacacttacc catctggaaa actgacatgt gtcgtcagtt ttccagacta
    23341 gcctttagaa tacctatagg aataaaaagt tacttttgtg caggctaatg ccactagcag
    23401 cctacaattg cgacatccaa agaatcccat taattaacac ttcccgagtt tgtttcagtc
    23461 taccgttaaa accatcgtaa tcgcatcagg tgtattgcgt ctcatcagga tcgatttccc
    23521 catttcgata tcagacccgt cgccctcaga aaatctcttc ttccgaatat acttatcaaa
    23581 tctcctctca ggcatacggg aggaataatg ttcactctgt cacattatct cccctggcac
    23641 aggcatattc tgttgttatc aaccgtatat gatgaagtca tctgagagaa ataatatggc
    23701 tcgattgata acgacaaccc cggtatcaat cggcggaaaa atatctttct caggagcagt
    23761 aatccatttc aacgaacaga gaacacttcc cccattgcct gccttgtatg atgcagcagg
    23821 atactatgtc aactcagtga acctctggtt catttcactc gtttcagaaa gaatgcttca
    23881 ggacctcagt tcctgttcca gagcactttt acgctactgg cgttttctgg agcgcacaga
    23941 tatacaatgg gactttatgc ctttttctcg tgcggataag cccctttacc tttttcgaaa
    24001 tacagactta ttgcctgcaa tcaataatgg agacctggcg tattcaaccg ccagtaccta
    24061 catggggcat attattcagt tttataattg ggcagtatca aacaacctgt taaaaacaga
    24121 cgagaaacat gcaccgttcg gggtggatat tattcacatt cagaaccccg atgaacccgg
    24181 aaaatttcag cgacgctcga tgaccatccc ttatgacctc cgaatccgca gcccccgaaa
    24241 aacaaatatc aatggtgccg cgtctctgaa tcccttaagc cttgaagaat tgtctctgat
    24301 gtttcaccaa ctcccgtcaa catctgttga attccgattg atatgcacgc tggcgctaca
    24361 gtcgggactc agactggagg aggcttgtgg attcactgtt gatgctctct ttcaggcagc
    24421 cccaaccagc catctcaaaa accggtacct tattcggatc ggccctgaaa atgggattcc
    24481 aacgaagtat tctaaaacgc gtaaaattga aatatctgct tccttactgg aaagtctgaa
    24541 ggaatatgtc atacaggagc gcagattacg ccgactgaat acactattaa acagacgatc
    24601 tgttgcaccg gaaacgaaga atacccggtg ccggttcgaa cccctgttca tcagccagca
    24661 gggaaatcca gtcactccgg cagttttcgc tacccgctgg gcagagttca ggacccatct
    24721 aaaaacgttt gtcccaacgt tcagacataa attccacgat ttacgctgca cctacgccac
    24781 tttgcgtttg caacacttac tcgatgctgg cctggcccca catgaggcta tggatctgct
    24841 gatgggatgg atggggcata acaacgaagc caccacctgg aagtacctga cttatctgag
    24901 atacgaaaca ctgcttaagg ataaatttga gatgatggat aacatcatgc acctcaccct
    24961 gaaggagcct cccggtgata aataacccct gggatccaca cctctgtctt aagtgtttgc
    25021 acaatgagcg aaaaaatttg cttctcgact ttaatcgcct gagatacctg catattcccc
    25081 tcactcggac gattcagggg tgcggtaagc taacatttgc ccaccgagac ccgtgggttc
    25141 gctctatgat atcagcatta aaacaaagaa atgatttaag cagcgccact aaagggacgc
    25201 tatttacgtt catgatatcc cacacccggt tatgtgaccg tgctcaactg acgcctttct
    25261 ctgcggaagc cgtgcagttg caggcacagt tctttactga ccaaaacagg aaaggaaagc
    25321 tgaaagatac ttctctggta ggcctgcgtt cccagctctc atcctgtctg aacctgcttg
    25381 gtcttccagc cgaagcactg atgagcgatg tccctgtctt cagtcgatta cagcgtgtac
    25441 cggtacaggg ttacagcacc acagagctca gacgcttact gccgttactg cggggaatgt
    25501 ttaaacagct atcaggtcag tttatcgctg accctgcaaa acacatcaac gcctacagaa
    25561 ccactccgtc catgcacttc aactggcaag gagtgattta cccggtctgt ggagggatca
    25621 gcaagctgat gtgctgcgcg ctttatcttc tggcctacta tacttggtca aatacttcgg
    25681 ttcttttgtc cctgaagcga ccacataaca gtgcacgttc tgtttcagag agctggtacc
    25741 ggatgccggc gtttaagaga cgggctttca gaacagtgac cgtttatttc accgggcatg
    25801 accacctgga tattcccaaa tattcactgc agttctttga ccagctcacg gccgtttccc
    25861 gcctgctgag cgatgaaccc gaaacctttc tcctcaaaac agcagtcaac ggtaagattg
    25921 tcccctttcc aataaccgca ttatcgacat ttaatcggcg atttatttcc ggtgttctgg
    25981 ggattaaaga tgacagcggc gcgcctcttc atctgactgc cgcccgcttc agggcgaccg
    26041 ggagtatgct ctcgcttacc agatatgatc cgctgcaaac cagtgtgtta cttgataaca
    26101 cacctgcagt tgtaaatacg cactacgcca gtggaaataa gcacgataac aacagaatgc
    26161 tgcgtgacgt caccgccgtg ctcgaatacc agtcacgaca ccgctcagga atcgaagccg
    26221 ctgaagatga gctcaggaag caactggaca tacctgtact gacgtataaa gcctacctgg
    26281 aaagcgtctc ccgccccctg agaagtgcac atggatgcta ctgctctgct cctgaaggta
    26341 ctgaaagtcg aaaattcaca ctacagtccc tcacccatca actggtcggt aacggcgagc
    26401 tactggcctg tgccagtttg ctgaactgtt ttgcctgtcg acaccaggtg cttgtggagt
    26461 cagagaccga tatatggtgt ctgctcagct tcagagacac attgcggaat tcccgggaac
    26521 accatcttaa tctgaatcat taccagaaaa atttcctgac gttgctcagt tcactggatg
    26581 aaagaatcaa actgctgaac accgatatgg tgagacgagc agaacggcgg ctgaaaaatc
    26641 acggtcctca cccactctgg acaacggagt aaattcctgc aggaggcaac atcgttatgg
    26701 catacaaagt cccgccgctg gccattgaaa tccctgaggg gagttcgcag gcatttatac
    26761 caggaacatc agacattatc gccctggtaa aatcaggtaa ccggaatgca ctgaacagga
    26821 ccgtcatctg ttatgacaat gacgggcatc ccgtcgcttt atttggccaa gacacatgga
    26881 actgcaggcc ttatgctcac acaaaggaat ccagagtaag caaaaatcga ctcagatttg
    26941 cttatctgaa ttcatgtccc gaccttcagc ttgaagcgaa aatcattgtt tatggctggt
    27001 tatttcatcg ctcatccacg cgttcgcgtc cggctaagtt ctcctcgata gaatcccgtt
    27061 gcaatacacc gcttaatgag attttttctt ttttagcgga aaacaagcac gacagtatcg
    27121 tcgcgctgaa tcagaccggg gtatggcagt cctttcttgc gtgggtgcaa gctaaagact
    27181 ggtctcatat ttccgtcggg catgtgttgc ggacgctccg ttttattgcc aaaaacgcct
    27241 gttggcataa catcagactg atacttcccg aatttaaggt cgacgacctt gctggacgac
    27301 tctgccacaa cgaacggcgt acacatcagc agattctggc tctcccgcaa tctgtcgcag
    27361 acaccctcta cggtgaagct attcgctttg tcgaatcagc atggccgcac agacttgcac
    27421 tggaagaaac cgaaaggcgg cttcaggaaa actatctcca ggctaaatcc atcgtggatg
    27481 cttatcttgc tgagggtcat tttcctgccc tagttaatcc tgaaggggaa attacagata
    27541 agcaccggta cagtctgacc ataaagtctt ttctgcgcga gcgtgtgcag gatacgattc
    27601 attcctgtct cgggccctcc ggactactcc ccggggaaaa agccgacggt gactggctta
    27661 aaaagttccg gggacaactc ctgacgtcct gtttcatttg ctgcgcggcg ttcagtggca
    27721 tgcgactcag cgaacttttt gaactgacaa aagacagttt tcatactgag gtcatcgggg
    27781 gagaaacatt cttccttctg aaaggcagaa ccttcaaatt tggcgaaaag caggagacct
    27841 gggtcaccag cccgataacg gagaaagcca ttcaactggc cacctccctg acccggctgg
    27901 cccgatgtca gttggaggac gctgcaaaat ggagtactgg aatctcagaa aaagaacact
    27961 acctaaatct ttcacgttca ctctggctga gccagacata ccgttcacgc cttcccgttc
    28021 tgattattcg ctggaacgcc cgtctgcagg cattttccag gcaagtctca gccaccattg
    28081 ataacgacac actgagggaa tgtattaccc tgaatccaaa cagtaggcta cggacagaaa
    28141 aactccagaa cagtaacacc aactggccaa tgaccactca tatgttcaga cgaacactgg
    28201 ccgtctttgc tgtcaaaaat aacttaggaa gcgccattgc catcaaacag cagtttggcc
    28261 atctccggtt gaaaatgtcc gagtggtatt gcgaaggagg gattgcatcg cggatgaaca
    28321 atgtgcttat cgatacagaa cttcaggatc tgattgatcg cgaacgactc gataataata
    28381 ctcgcctcta ttacgactgg ttcaatggca ttacgccgct cagcggaaca catggaaaag
    28441 ccattgttgc catgcgtcag gacttacctg tggtgtatcg ctcttgggaa agcatttggc
    28501 agcttctcag cacgggcagg ctcactcttc acggtacact acatggctac tgtaaaaacg
    28561 gttataactg cagtatggat ggtgttgtta acccagcatt ttgtgtaaat tgccgcggag
    28621 gtggaagcat aattgacgcc gaaaacgccg cctggtggca aaggaagcac gcacaactga
    28681 tagtatggct gacagagaac ccaaccttct caccccagga atatgcccat tgcatcacac
    28741 agatcagggc agcagagaat gttatgcagg attttgacct gtcgtttcag tgctatgagg
    28801 gcaaaggaga acagagatga aggggtctga caacgcacaa ctcaggctgg gatctgcact
    28861 cacccgcctg cttgaaggca ctccgatgca tattcaggct gaccgaaaac tcagtgtcag
    28921 agccgttgaa gaggaagcca gactcggaaa cggcagtgca tactactacc ctgaattgat
    28981 cacccgaatc aaagttgcca tattgcaggg ccggaaatgc gggccagcag caccctcacc
    29041 tataccgacg ttgcgagccc ggctcaataa gacacaaaag ctgaaactgg cccttcgcga
    29101 ggaaaaccgt gcgctgaagc ttactgtttc aaacatggca gcagaacacc accggctggc
    29161 ctgggcattg cgggaggcac atcttcgcat tgaggaactg gaagtcgcat tgacagaagc
    29221 gaagcgggca gggatacacc gactaaaata atacaatctg acgactgaac tcgtaagcgt
    29281 acagcgagga ccgtattgac gaagaaggct attccacagg cagtgtgacg cgccactgca
    29341 gcagttcact gacccagttg actggccagt ctgcgaaaaa aacaaacaca taatgaaggt
    29401 agtgttctgg atctattcca ttcagtttgc acgtccaaat cagactgcgc agcagcgcac
    29461 cgcgctcacc tccgagatct gagccgacgg acaaccagtt tttcctgccc aggcagatca
    29521 gccgtagcgc gtggcaaaat tgttatccgc ttcagcccag ccatagggcc ctgcatgggt
    29581 ccatgtttgc tcgcagcatt agatgagaac cgttttaatt gtatcgagga aatacagaac
    29641 tatgcaggta ttgcgcctgt aaacgagcgg agaggccaga aatcctagtt atactggcac
    29701 tggcaatgtt ccaagttcgt cctgcagaca ttcgccgaat gggctgctaa aacggcaaat
    29761 tcatctttct gggcaaaact gtatatcata gcctcaggga gacgggttta gttgtaggtc
    29821 ttccagtgag tgattttgca cttctgcttt aacacggtat ggtctgtatt atcaggatga
    29881 tgcgtgatct gatcctgcaa cttagctaaa gttagattta ttcaactaaa cctaaaaata
    29941 cttaaacttg aatatgaaaa caacttatta aaaaataaac tgaaaattta aaacttcaat
    30001 tggttaataa taaaagacaa tgcaataatt ttcttaaatt tcccgtcacg tcgatgaaaa
    30061 ccattgaatc ttaggttgga aaactggccg taacaagccg gttttcacat aaaatcaatg
    30121 tttttactca atcttgaaat cgttatcagg ttcagacgtc gattaaagca aaatcaaata
    30181 actgaaaata aaaatgcgtc gatcacctgt tgatatagag aacccatgtt tagttgtttt
    30241 gttttgtcta aaacagcagg tggcgatata gttatttcac tgaaattcat tttcaaatta
    30301 aactctccga tttttgacat accatttctg cgcactattt ctggggatgt agactcagtc
    30361 tcatgtatag ataatgacca ctcatccgat tgtttctgca gtatcttata gtcttctggc
    30421 aaatcttcgg ccagtctatt cgagaataaa gtcagttcat cagtagcgat agtaaataac
    30481 caggcttctt gattttcact ttgattcacc cgtaaaattc tacccaatac ttgtctgaag
    30541 taaagttctg ttttgacaga acttaggtgg cagcacacct gtagtctagg gatatctgtg
    30601 ccctcactaa tcataccaac gctaacaatc cattctgtag ttgattgccg ataattctct
    30661 atcttaacca aagcatctcg atcgtggtac gtcacaattg tcgcagtttg ggcgaattcg
    30721 ttttccaata tattcagtag ttgatatgca tgttcgacag aagaagcgac aactaaacca
    30781 cccgcgtcag aattaacctt acgtatttca caaagttttt tacatccaga tttgagtaaa
    30841 tacttcattg catctggatg ccaaattatt gattgatacg aagtgatggt ttcactcagg
    30901 aaacctgcta tggaatcaaa atgctgaaca atttcccctg atgaataaag taaattatta
    30961 ctatttataa gagcaatttt aggtttacga caaaccccat cgacaatagc ctcgtgtaga
    31021 ccatatacat agtcacaaca gatcttccct tgcggatcag tgtaatttga aagcactata
    31081 ggattattat ccgttctcca tggggtccct gttaatgcca aagtgtaact ggtacattgt
    31141 tgaactcgat taaggatttc cccaccccac atattagcat tattcagaga atcgccagaa
    31201 caatgatgga tttcgtcaaa aacgactaat acccgatgca tctgcaaaga gtgccagaag
    31261 ctcttatcga aatacatcag actatgataa gtatacacag cacctactgc gccaagacca
    31321 ccattaaaat tcctttgaag aatatgagag aaagtacttt caatggatga aaccaccaca
    31381 gacgaaggtg caaagcacag gatgagatct accagtttaa gctccagcat tttcctagca
    31441 acttcagcag ccatcaccgt ttttccagca ccaggactag caagacatag aaagtgcggc
    31501 gatccagatt gatatttttc taacgctgct tcagagcatt ctttctgcca ctccctcagc
    31561 atgcgcaatc cccattgcta agctggccta acactttcga taaagcagta accctgccaa
    31621 gcaaagcggc ggactgattt tttgcgtgct gatgtagttc aataaagcag ttccttctac
    31681 tgggaaaacg ctccataaga gactggtact cttcaatttc actcagaatg acggcaagtt
    31741 tggcctcatg gacagttttt tcttttctca ggtccattaa gaaaagatcg acatccaaaa
    31801 cggatagttt cacattttcg ggcatagcag ctaggcgcga gttacttggc aattttcgtg
    31861 gtatgaaagt cgttgaatag aaactttccg tcttcttgta caagttcttc gggcgtccat
    31921 cactattttc gagtttcgaa atgaaaccca atgcctcaag ccggagaagt tgtctgtgga
    31981 tgaattttct tgcttctgtc acatcagtat acgaatctgt aatcttcagc aaagcgtccc
    32041 tgagatggat agcaccgaac tctttaaaat ccttttttat caacaaatta taaataaaag
    32101 ggtgcaactt cactgcagac ttcattatta aaacatccaa atcagaaaac ttagaattgc
    32161 taagtataca gaaaccaacg aaacttagac aatctaagta ccttaaggca atgatgatga
    32221 ctacgtggct cacccaaatc cgatacctga caggctcaaa gcagccagaa caaaagccgg
    32281 gatcactcag aaagaattgg gtatccgaat tggcatggaa ccaagttcag cgagcggccg
    32341 catgaaccac tacgagaagg ggaggcatac tcctgacatt gatactctca agcgtatggc
    32401 cgatgaactt ggggtgccga tcagctactt cttttgcgag aacgaaacga ctgcagagtt
    32461 agctcgcctg atcgctaaca tgtcagaaca acagcaagtt gaactgatca aaattctgaa
    32521 gaaagaccaa cgttaaaaag cttaactgcg ctgcagttga agaaaccacc tcttacccag
    32581 actgagagtt cttcttcagt agcacaggga gattaagacc cagcatcttt ccgcaaactt
    32641 tccctacaca catatctgtt tcatagcaga gatcttgggc gcttagctca tcgattttag
    32701 caccataact tgcgctcatt atggccaaga acatagtgat gagactgaca tttgtacgca
    32761 ggttgcatgg gtaatccttt catcaaatag cctaaaaaag acaccattgg attttaactt
    32821 caaaaaaaca acaaaagcag aattaacatc cataaattat tttaatttaa gtttttttga
    32881 cttaatttta aatcactgat tatttgtaca ttgaaaagga aaaaaacaga catatctaat
    32941 agctatcttt ccgaggggag ccggctatgt ctgacataag tgcaggaaat cactacggtg
    33001 tgcggatact aagtctgaat ggcggtggag ctaggggtat gtttacaatc agtgtccttt
    33061 ccgagattga gaggatactg gccagcaagc accctaatca ggatattaaa attggcgact
    33121 attttgacct cattacaggg acgtcaatag gcggcatatt ggctctcggc cttgctacag
    33181 ggaaaagtgc ccgagagctg gaacgagttt tcttcgatag ggcgaataac atttttccca
    33241 aaagatggtc cctgaccaac ttgcttagat cactttgtgc ccctatctac cgcagcaatc
    33301 ctcttcgcga aacaattcaa gaaatgattg gtgctgaaac cacattcaat gaccttaccc
    33361 ggcgagtaat gatcccggca gtaaatcttt caacaggaaa accgcagttt ttcaagacac
    33421 cacacaaccc agattttact cgtgatgggc ctcttaaatt gatagatgct gcactcgcaa
    33481 cctcagccgc gccaacgtat tttgccccac actattgtga ggatctccgt gcgtattttg
    33541 ccgatggtgg tctggtcgca aacaatccca gctacatagg acttctagaa gttttcaggg
    33601 atatgaattc tgacttcagc gtcacccata aagacataca tatacttaat atcggcactg
    33661 ttggtgaaga gtattcactt agtccaaagt tgttgagcaa aacatggtgg aatggctact
    33721 gtcgtctctg gggtatggga aaacggctgg tgcttacaac catgacagct aaccagcatc
    33781 ttcataaaaa tatgctacag cgtgagctta tgctccatga cgcggtggat aactacatct
    33841 acttggatga aattatcccc aatgaagcag ccagtgatat cactctagat aatgctacga
    33901 agagttccct tgaaaattta agcgcaaggg gaaggcagct cgccaatgtt aagttcgctc
    33961 aagatcagaa acttaagaat tttttcacta ctcccgcaaa accctttaag cgcatgaatg
    34021 cgcaggagat ccaataatgc gttgggattt taacaattat tacagccata gtgtggatgg
    34081 cttaatcagt aaactaaaat tgggtaagag tgaatccgac aaactaaaag cacttcgcca
    34141 gaaagtccgt gaaagaacga gggatgtgtt taaagaagct cgtcaaattg ctacagacgc
    34201 cagaaagcaa actctgacgc ttgaaagcgt tagatttaaa ctggagcaaa ctaatgttcg
    34261 ctacctctct ccctcagacc gtgagactct cgctcgactc atttttgaaa tggaagatgt
    34321 agctcgtgat gacttcatca aatttcagcc acgcttctgg acccaaggaa gctttcaata
    34381 cgatacgtta aacagacctt ttcacccggg tcaggaaatg gatatcgatg acggcaccta
    34441 catgccaatg acagtatttg aatccgaacc cagtattggg cacaccttac tacttctgct
    34501 cgttgatacg tcactgaaat cgttagaagc tgaaaacgat ggatgggtat atgaagaaaa
    34561 aaacacctgc ggacgcatca aaattcctca tgagaaaaca cacattgacg ttccgatgta
    34621 tgcgatcccc aaagagcaat ttcagaccaa acaaactgct gccgattccg cacacatcat
    34681 aaaaagtgcc gatgccatgt tcgagtcttt atcagcaaac atggatacgc gcgaggcata
    34741 tctagtagag cctgacaaag ttaatttagc gctacgtcaa ggtgcaagaa ggtggtcaat
    34801 cagcgacccc aaaattgttg aagattggtt taatgatagt tgcaagcgta ttggcggtca
    34861 cctccgttca atttgccgct ttatgaaggc atggagagat gcccaatggg aagtgggagg
    34921 tccttcatct attagcctga tggctgcagt ggtaaacatc ctcgacagag aagcacataa
    34981 tggttcagac ctcactggga cgatgaaact agttgcagga ttactaccta atgaattctg
    35041 tagcggtctg gaaagccccg atgatactga tgaaaagtta cttttccctg ccgaatgtga
    35101 tcataacgtg catcaaagaa ccatcgttga aaccatgaga aatttagagg ggattttgct
    35161 tgatgcggag agttcaaata gcagagaaga agcattacgt aggataaacg atgcgtttgg
    35221 aaaacgagta actaatgctt cgttaataat ttcaattgct gcggctcctg catttcaaat
    35281 ctccccgtct aaggagcagg cgcctgtaaa aattaacacc actatggtaa gcggctgatg
    35341 gaggaaggaa gactccataa aatgatgata gagtgtgggt atcgctatac gctagcccgc
    35401 aaacttcctg aaaaggcttt cctacacagt caggaacgcc tgaaaggcta ttatacgaaa
    35461 gaatatgaaa ccgaagccgg aaatttcaat gtagcactag ttattcggcc agatcctttt
    35521 acggaactgc cattagcttg catagttgat ctgcctgaac agttcagaga tcgccttatg
    35581 cctcacataa gccaagaagg gattctctgt tatgtagagc agttggaagc tgattgggat
    35641 ccaaacaacc ttgagggtac gtacaaagaa gtcgatgccc agattcaccg aacacttatt
    35701 aattcagttt tagccgctac tactggtctg aatgataaga gagaactgga aggtgaattt
    35761 gcaacttact ggcgacctag tgatagttta tatattttat caaaagcaag ccgcgggatc
    35821 agacttaaga cactgttttc agagtcaaca tttcctgatg gttctcccag gctagaatat
    35881 ataactgtcc aggaatcctc tcccgaggac tctgaaacca ttttaaaaaa atggct
//
LOCUS       DAOFQD010000040        35599 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.040, whole genome shotgun sequence.
ACCESSION   DAOFQD010000040 DAOFQD010000000
VERSION     DAOFQD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.040"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(1..77)
                     /locus_tag="QBF46_001996"
     tRNA            complement(1..77)
                     /locus_tag="QBF46_001996"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Arg,seq:ccg)
     gene            complement(180..1565)
                     /locus_tag="QBF46_001997"
     CDS             complement(180..1565)
                     /locus_tag="QBF46_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6674182.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1756..2496)
                     /gene="rffM"
                     /locus_tag="QBF46_001998"
     CDS             complement(1756..2496)
                     /gene="rffM"
                     /locus_tag="QBF46_001998"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDQ6674183.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2499..3851)
                     /gene="wzyE"
                     /locus_tag="QBF46_001999"
     CDS             complement(2499..3851)
                     /gene="wzyE"
                     /locus_tag="QBF46_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDQ6674184.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3848..4927)
                     /gene="rffT"
                     /locus_tag="QBF46_002000"
                     /gene_synonym="wecF"
     CDS             complement(3848..4927)
                     /gene="rffT"
                     /locus_tag="QBF46_002000"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDQ6674185.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWKDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(4924..6174)
                     /gene="wzxE"
                     /locus_tag="QBF46_002001"
     CDS             complement(4924..6174)
                     /gene="wzxE"
                     /locus_tag="QBF46_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDQ6674186.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6176..7306)
                     /gene="rffA"
                     /locus_tag="QBF46_002002"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6176..7306)
                     /gene="rffA"
                     /locus_tag="QBF46_002002"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDQ6674187.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7311..7985)
                     /gene="rffC"
                     /locus_tag="QBF46_002003"
                     /gene_synonym="wecD"
     CDS             complement(7311..7985)
                     /gene="rffC"
                     /locus_tag="QBF46_002003"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDQ6674188.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(7963..8844)
                     /gene="rfbA"
                     /locus_tag="QBF46_002004"
     CDS             complement(7963..8844)
                     /gene="rfbA"
                     /locus_tag="QBF46_002004"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDQ6674189.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(8863..9930)
                     /gene="rffG"
                     /locus_tag="QBF46_002005"
     CDS             complement(8863..9930)
                     /gene="rffG"
                     /locus_tag="QBF46_002005"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDQ6674190.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(9930..11192)
                     /gene="wecC"
                     /locus_tag="QBF46_002006"
     CDS             complement(9930..11192)
                     /gene="wecC"
                     /locus_tag="QBF46_002006"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDQ6674191.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11189..12319)
                     /gene="wecB"
                     /locus_tag="QBF46_002007"
     CDS             complement(11189..12319)
                     /gene="wecB"
                     /locus_tag="QBF46_002007"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDQ6674192.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPMHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12375..13421)
                     /gene="wzzE"
                     /locus_tag="QBF46_002008"
     CDS             complement(12375..13421)
                     /gene="wzzE"
                     /locus_tag="QBF46_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDQ6674193.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13433..14536)
                     /gene="wecA"
                     /locus_tag="QBF46_002009"
     CDS             complement(13433..14536)
                     /gene="wecA"
                     /locus_tag="QBF46_002009"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDQ6674194.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14776..16035)
                     /gene="rho"
                     /locus_tag="QBF46_002010"
     CDS             complement(14776..16035)
                     /gene="rho"
                     /locus_tag="QBF46_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDQ6674195.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16120..16221)
                     /gene="rhoL"
                     /locus_tag="QBF46_002011"
     CDS             complement(16120..16221)
                     /gene="rhoL"
                     /locus_tag="QBF46_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDQ6674196.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16362..16691)
                     /gene="trxA"
                     /locus_tag="QBF46_002012"
     CDS             complement(16362..16691)
                     /gene="trxA"
                     /locus_tag="QBF46_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDQ6674197.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16822..18087
                     /gene="rhlB"
                     /locus_tag="QBF46_002013"
     CDS             16822..18087
                     /gene="rhlB"
                     /locus_tag="QBF46_002013"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDQ6674198.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18223..19707
                     /gene="gppA"
                     /locus_tag="QBF46_002014"
     CDS             18223..19707
                     /gene="gppA"
                     /locus_tag="QBF46_002014"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDQ6674199.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19754..21775)
                     /gene="rep"
                     /locus_tag="QBF46_002015"
     CDS             complement(19754..21775)
                     /gene="rep"
                     /locus_tag="QBF46_002015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDQ6674200.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            21992..22441
                     /locus_tag="QBF46_002016"
     CDS             21992..22441
                     /locus_tag="QBF46_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDQ6674201.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22640..22921
                     /gene="ppiC"
                     /locus_tag="QBF46_002017"
     CDS             22640..22921
                     /gene="ppiC"
                     /locus_tag="QBF46_002017"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDQ6674202.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23008..24483)
                     /gene="ilvC"
                     /locus_tag="QBF46_002018"
     CDS             complement(23008..24483)
                     /gene="ilvC"
                     /locus_tag="QBF46_002018"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDQ6674203.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24633..25523
                     /gene="ilvY"
                     /locus_tag="QBF46_002019"
     CDS             24633..25523
                     /gene="ilvY"
                     /locus_tag="QBF46_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDQ6674204.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QALFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(25520..27064)
                     /gene="ilvA"
                     /locus_tag="QBF46_002020"
     CDS             complement(25520..27064)
                     /gene="ilvA"
                     /locus_tag="QBF46_002020"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDQ6674205.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLLRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27067..28917)
                     /gene="ilvD"
                     /locus_tag="QBF46_002021"
     CDS             complement(27067..28917)
                     /gene="ilvD"
                     /locus_tag="QBF46_002021"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDQ6674206.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(28982..29911)
                     /gene="ilvE"
                     /locus_tag="QBF46_002022"
     CDS             complement(28982..29911)
                     /gene="ilvE"
                     /locus_tag="QBF46_002022"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDQ6674207.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29931..30194)
                     /gene="ilvM"
                     /locus_tag="QBF46_002023"
     CDS             complement(29931..30194)
                     /gene="ilvM"
                     /locus_tag="QBF46_002023"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDQ6674208.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30191..31837)
                     /locus_tag="QBF46_002024"
     CDS             complement(30191..31837)
                     /locus_tag="QBF46_002024"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDQ6674209.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFEVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31840..31890)
                     /locus_tag="QBF46_002025"
     CDS             complement(31840..31890)
                     /locus_tag="QBF46_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674210.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31977..32075)
                     /gene="ilvL"
                     /locus_tag="QBF46_002026"
     CDS             complement(31977..32075)
                     /gene="ilvL"
                     /locus_tag="QBF46_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDQ6674211.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32428..33948
                     /locus_tag="QBF46_002027"
     CDS             32428..33948
                     /locus_tag="QBF46_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDQ6674212.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEHGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(33973..34311)
                     /gene="maoP"
                     /locus_tag="QBF46_002028"
     CDS             complement(33973..34311)
                     /gene="maoP"
                     /locus_tag="QBF46_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDQ6674213.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34430..35269
                     /gene="hdfR"
                     /locus_tag="QBF46_002029"
     CDS             34430..35269
                     /gene="hdfR"
                     /locus_tag="QBF46_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDQ6674214.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(35365..35440)
                     /locus_tag="QBF46_002030"
     tRNA            complement(35365..35440)
                     /locus_tag="QBF46_002030"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35405..35407),aa:Trp,seq:cca)
     gene            complement(35449..35525)
                     /locus_tag="QBF46_002031"
     tRNA            complement(35449..35525)
                     /locus_tag="QBF46_002031"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35489..35491),aa:Asp,seq:gtc)
BASE COUNT         8678 a         9714 c         8997 g         8210 t
ORIGIN      
        1 tggcgcgccc gacaggattc gaacctgaga cctctgcctc cggagggcag cgctctatcc
       61 agctgagcta cgggcgctta gcgccgttgc gggggtggat aatacggact tcccacccct
      121 ctgtctagtc cctttttaaa taaattgcgc gtttggttac ggtttgtgcg ttttgctgct
      181 tattcctcca gtttatgcgc tttcccgtgg cgattaaggc caaaaacttt ataaatcgcc
      241 gtcaccgcca gcataaagat gatgccaaca aacagcgaca tacgcgtatc ttcattaaag
      301 tacatgccga tcaaaacgca aatcaggaat gccattgtta cgtagttggc ccacgggaac
      361 aggattgagc ggaacggatg gctggcaatc gccgctttat gtgcacgccg aaaacgcagc
      421 tggcttatca atatcacaaa ccatggcacc atccccggaa gcacgctggc actgtagacg
      481 tagacaaaca cacgctgcgg attgggaatg atgtagttca ggcatgagcc aattagcaga
      541 atagcaatag atactgccac acccgcaacc ggtacgccgt gacgggaaac tttcgccatt
      601 gccgccggta actgacggtt tttcgccagt gcgtagagca tacgtccgca gctgtacatg
      661 ccgctgttac agccagagag cgcagccgtc agcaccacaa agttgataat gcccgccgct
      721 gcggtaatac cgattttggc aaaagtcagt acgaacgggc tgccgttgct gccaatttca
      781 ttccacggga agatggtgac gataacgaaa atcgcgccta cgtagaaaat caggatccgc
      841 cacagcactt tgcctacggc actgcgcagc gtcacctgcg gattcttcgc ttcaccggca
      901 gtaatgccaa tcagctccac gccctggtag gacgccacca caatacacag agcggtcagg
      961 aaccctttcc agccacccgc aaagaaaccg ccatgctctg tgagattgct aaaaccaatc
     1021 gactgcccgc cattgccaaa gccaaagaaa atcacgccca ggccaatgac aatcatcacg
     1081 ataatcgtgg tgactttgat catcgcgaac cagaactcga tttcgccgta caaccgcacc
     1141 gccgccagat tcgccaacgc caccagcgcc accgcgatca atgcgggtat ccactgcgcc
     1201 atctccggga accagaactg gacataaacg ccaatggcgg tgatttctga gatccccacc
     1261 gccatccaca taaaccagta agaccaggcg gtgagataac caaaaaacgg gctcatataa
     1321 cgatgcgcat aaacggcgaa cgaaccggta accggttcga ggaacaacat ttcgcccatt
     1381 gaacgcatga tgaaaaagac gaacagcccg gcgatgatat aggccaacaa tacggatggc
     1441 ccggcccatt tcagggtact ggcggccccc ataaacaggc cgacgccaat ggtgcccccc
     1501 agggcgatga gttcgatatg tcgagcttcc agcccacgct gtagctctgg tttgttatct
     1561 gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt taccggttat cgttatgggt
     1621 acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat ggcaaataaa gcattaaaaa
     1681 tttgaatgct ttgtgtaata aaaaagcaga caggcgacga agtgaccact ccgtcgcttt
     1741 acaaagagag gaaaatcaga ggttgccggt gtaatgccag cgtaaataac gcagcaaacg
     1801 aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg tagagccact ccagtcccag
     1861 cgtttgccag attttcggtg cgcgttttac gtgaccggtg aaaacatcgt aagtcccgcc
     1921 aacgcccata tacagcgcat ctggatgcac cagacggcag tcgcgcatga agatctcctg
     1981 ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg ctggcatgaa tgcgttcaaa
     2041 cagcgcctga cgctgctcgg gtttaaaata accatcctga ctgccaacga tattcacatt
     2101 ccattggttg cgcagtttag cttcagtttg cgccagcact tcaggtttac cgcccacaag
     2161 aaataccggc gtcccttctt tgcctgcgcg cgccatcagc tcttcccaga gatcggcacc
     2221 ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt actgaacgta caacgctgat
     2281 gccatccgca tatttaaatt cggcagcgtt aattaactcc ctgacctcgg cgttatcttc
     2341 aatggtcagc attttttcag cattaatggc aaccagcgtt ccctgcttaa gctgcccgtc
     2401 agcaaacaga taatcgaggg cgtgctgcat atcacgccaa ccaatcaact gtaagccacg
     2461 cagcgtatag gtcggtgccg tggtgttgtt attcattgtt atccttcaac ctgcgtccgg
     2521 agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt caaaaagcca gtacaacagt
     2581 tttgcgatca tcagacatgc gccgaagacc acgataaaaa agactacgcg tgagacaaac
     2641 gaatccagcc cttcacgtgc cagcacgatc atattgaaga tcgccccaaa gcagaaactg
     2701 tgcaatatcg cagccttata gcgattagtc tcgcggttgc ccagctcata cagccagtcg
     2761 aaccatttga tgatcagtcc aaccacaatc gccccgagcg ggatgaacaa cgcgccgccc
     2821 atcaccacca gtgagcctat aagcgtaggc gagatcgcca gtccggagtg gttattcagc
     2881 acttcccagg taaagtagtt ggctgagttc agcaccatac tcgggcgccc cggccacagc
     2941 caggaaggga taaagacata gaaatcgcgg acaattggag ccaggccctg gaagtcgatg
     3001 ttgtcgtagt tctgcaacag caacgccaga ttctcccacg gcgagaaggt gtcgcgagtg
     3061 agatagagaa acgtatagaa cgcttcatcg ccgctcacat tcattccata gcgttttaac
     3121 gccagccaga acatgccaac aatccccagc acgcccgccg ccgccagcat ccacaacgaa
     3181 atccagccgc gaataatgcc aataaacagg aagatagcga atgcgatgat gatattggcg
     3241 cgagtgccgc cgacaatcat ataagtcagc aagccaaaag cgaccgtgct gacgaggaaa
     3301 aacagccacg ctttgctgtc ctggcgcaga aagtagacca ccagcatcgc cgggatgaaa
     3361 aagtaaaaga agcgttttaa cgccacgccg gagacttcac tggaaaagat ctgactgtag
     3421 gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga agatgccgac gcttaccagc
     3481 gcgataccca tcaggatcac ccacgtgaga ttggtctcca cgcggttcat ggtaaacagc
     3541 ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg ttttgtaggt gacgtagtaa
     3601 accgcgtaga agcagcccgc agaaagcaac gcctgcaaca agatttctgg cggcgcgaca
     3661 ccaacatcaa agcgaaatac cagcacgctg gtcagcggga agccgaagaa aaaggtgagc
     3721 aaaaacagca atgaaaagaa gacattgaag ttaaaacgca cacggcgaaa ctcaaaccag
     3781 gtcagcgtgg caataaacag cgtgcagagc agccagacaa caaacaggcc actgaattgc
     3841 agcagactca tgcaacctcc ccggcggcaa tagccaacgc ccgctgccag ccttgcagat
     3901 agttcgggct aaagaaggca atggtgtttt tatccaccga cgccaactgg cgctgcgctt
     3961 cacgcacaat atcctcgttg agatcgtcgg tagtaaacag caccggcaaa tgttgttccg
     4021 tcatatcctt ccagaacgga ttttcccggt taagcacaca aggaatgccc gcctgaatca
     4081 gtaagcacag cgtaccaatg ccctgctggc gggcaaaaat aaagtaacca agatcgcact
     4141 gacgaagtag cgccagatag gcgtcaaatt ccagtttttc gctcagaatt tgtagatttt
     4201 cttcgctgaa taactccagc cccgcctgac gaacttcctc aatgtacgct tcgttattag
     4261 gcggatatcc catcggcacc accactttta ccgtatcgcc aaattgctga tgaacggcgc
     4321 gcaaggcagc aatatgctca ttgctgcggt cgccggagtt ccccaccaga atggtcattt
     4381 tcccttcacg ttgccgatcg ttcgccatcg tattgagcga agggtccatc cgcgtcggga
     4441 agtacagcag ttcgccccgc acctttgggt gcgttttggc aaaaaagctc aaatcgccgc
     4501 gggtggcaaa tacacagccg actcgctttt gcgccaggcg acgtagcggg taaaaaagct
     4561 tatatctcaa gccactggaa agctcataca ggtctgcccc ccagatatgc cagaaaaact
     4621 ggctgggctt aatgccacca ctcagcagag ccagccatag ctttggattg aactgaccgt
     4681 ggaagaaaaa acgctgctga cggttagctt ttgctttcgc gatgaccgct tccgccagcg
     4741 attttttccc agggaaaaat tgcacagaaa gcgccggaca gctatcactt aagccgtcgt
     4801 ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt cgcggccagc gcgtcgttga
     4861 aaaaccgcag aacggttcgg ttatggtgag ggatatccga tcccagtacg tgaatcagta
     4921 cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa caaagagaaa aatagacgat
     4981 ataggttgcc atataggcct gcgccgcgcc cagtgcacca tgcgcaggga ttagccagtg
     5041 ggcaaatacc atcaataaag tgaactggct gacttccgcc agaatataaa accgcagtga
     5101 cgctttggcg atcaccagat aaccaaagac ataagcgccc acttttaaca catcacccac
     5161 taactgccag gcaaagagat cgcgcatagc ggtaaattta ttcgacaaca gcagccagat
     5221 agcaaaatca cgcagcaacc agacggtaaa actcgccgcc gccactgccg gtaagacgaa
     5281 tttcagcgat ttaaccactt cccgtgtgat atcgcgcttt tccgttagcc gcgacaacgt
     5341 gggcagcaaa tatacgctga acgatgccgt aataaattgc aggtaggcat cggaaatact
     5401 gctcacccct tgccagatcc ccacctcatc ccagctatac tgcgccgcca gcagtttacg
     5461 catcatgatg taagcaacag gcaaggtcac cgacgtaatc aacgccatga gcgtaaattt
     5521 gctcaactgc cctgccagac cgttatccca gctgggtttc agatagctta acgggatgac
     5581 accacgtttg atcaacatga tggcggcagg aattaccacc agcgcgggaa tcagcgccag
     5641 acccagcaac gccccttcat aaccgcccaa acggtaactg acgtagtacg cgagaacgcc
     5701 aatcaagctg ccgacaatca gcgataacgc attacctgcg gcatcgcgaa agcctttcat
     5761 cagcgccagt aacaggttgc cccaggcgat ccccatttgc accagcgcca ctaaacgcac
     5821 cagcccctga tagtcggtat taccaaacaa tccctggctg attggcgcag ctgccagcac
     5881 aaaaaccagc gccatcagcg tagagaaacc aagtaccatc gctgatgaag tgccgaccac
     5941 gcggcgcagc tgttgcggat tatcatggta ctgggcaacg tatttggtta caccgttaaa
     6001 gatgccagcc ccggcaagca cgccgagcac ggtaatcaac tggcggaaat ttgccgccag
     6061 cccaagcccc gccggaccaa atgacaccgc cagcaactta ccgaccagta acccggcacc
     6121 aatcttgacc agtgtactgg ccgccgtcca caaggacgct tttgccaacg acatatcagg
     6181 aaaagtagtt caacaaagtc gcaattaccg tacgctgatt gacgggcgac aggttgtaga
     6241 acagcggcag gcgcagcagg cgctcgctct ctttggtggt gtagcgatct tcaccgtgga
     6301 actcaccaaa gtgttccccc gcagggcaac cgtgcagcgg aatgtaatga aacaccgcca
     6361 tgatttccgc ttctttcaga aagttaatca acgcgctccg gtcatcaata tcccgcagtt
     6421 taatgtagaa catatgcgcg ttctgcacgc agccatcggg aatcgacggc agctcgatac
     6481 gcccggcttt cgccagaggc gctaacgcat cgtagtagtt ttgccacagc gccagacgtt
     6541 gctggttgat acgatccgct gcttccagtt gcgcccacag gtatgcagct tgcagatcgg
     6601 acatcaaata gctggagcca atatcgcgcc aggtatattt atcgacctga ccacggaaga
     6661 actggctgcg gttagtgccc ttttcacgga tgatctcggc tcgttcgatt aacgctttat
     6721 cgttaatcag cgtcgcgccg ccttcaccgc ccgccgtgta gtttttggtt tcatggaagc
     6781 taaagcagcc aatatgacca atggttccca gtgcacgccc tttgtaagtg gacatcacgc
     6841 cctgagcggc atcttctacc acaaacaaat tatgcttttt cgccaacgcc ataatggtgt
     6901 ccatttcgca ggccacaccc gcgtaatgga ccggcacgat aacgcgcgtt ttgtcggtga
     6961 tcgccgcttc aatcagcgtt tcgtcgatgt tcatggtgtc cgggcgaaca tccacaaaaa
     7021 cgatttttgc gccacgcagc acaaaggcat tggcggtgga gacaaaggtg tagctcggca
     7081 tgatcacttc atcgccaggc tggatatcga gcagcagcgc cgccatctcc agcgaagcgg
     7141 tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg ttgctccagc cactgctggc
     7201 agcgacgggt aaaaccgcca tcgccacaca gtttgccgct acccattgcc gactgcatat
     7261 agtcgagttc ggttcccacc accggcggtg cgttaaatgg aatcatgtga tcacctgtat
     7321 aaccagtacg cggtgctttc tacattcgca ccactttgta tgtatcgttt aagcgcggcg
     7381 gtgttgccca tctgggtcgc cacccgcaaa gttgttttac cgcgagcata cgcccagttt
     7441 agcgccgttt gcatcagctc agcacctgca ccgcgtccag ccagcaggcc aattcgcgca
     7501 tctgtcgcat tgagttcccg taaagagaca tagccgcgaa tatcgccgga cgccgcacgt
     7561 aaaatcagac attgatgatc aaaggtgccg cgcacggcat tttcaatcca ctgtgcataa
     7621 aagcgactgc tggcgtcagg cgcataccac ggcgcacgaa aacggctttg cgcaaatgcg
     7681 gcgctggcta actgacgtaa tgcgggaata tcggtctctt gtgccactac agcaccgcta
     7741 tcactggcat tgttcacggg tagcgccaaa tcaacttcac cttctaccag ggagaatccc
     7801 agctgttgca gggcatccag ttcacccgta tttgatgccg caattttggc ctgcacccgt
     7861 gaccacggcg ctaacgcgtc tggcgtcagg agcggtgctt cagacgtaat gcgcacgatg
     7921 gcgctgttaa caccaaagaa ggcgttttcc caggttagtg gctcaatact ggcgcggacg
     7981 ggcacgaagt aactccagca gatattggcc gtagccagtt ttcgctaatg aactggcagc
     8041 acgcttcaca ccctcgtcat cgagccagcc gttacgccag gcgatctctt ccaggcaggc
     8101 aatcttaaag ccctggcgtt tttctactgt ctgcacaaaa gtgctggcct caatcaggct
     8161 gtcgtgagtg ccggtatcca gccaggcaaa tccgcgcccg agcagttcaa cggtcaggtt
     8221 gcccgcctcg aggtacatct ggttgatgga ggtaatctcc agttcaccgc gctccgacgg
     8281 cttcacctgc tttgcgtact ccacgacttt actgtcgtag aaataaagcc cggtcaccgc
     8341 ccagtttgac ttcggctgtt ttggcttttc ttccagcgag atagcgcgga aattgtcgtc
     8401 aaattccacc acgccaaagc gttccgggtc catcacctga tagccaaaaa ccgtcgcccc
     8461 ttccgtgcgc gccgcaacat gacgcagctt cggactgaag ccctgaccga agaagatgtt
     8521 atcgcccagc accagacaag aaggttcacc attaaggaag gtttcaccga tgataaaggc
     8581 ctgcgccaga ccgtccgggc tgggctgttc ggcatattcc agctggatac cgaactcact
     8641 accatcgccc agcaggcgct ggaaataacc tttatcttcc ggcgtagtga tgatgagaat
     8701 ttcgcggata ccggccagca tcagcaccga cagcggatag taaatcattg gcttatcgta
     8761 aatcggcaac agttgcttcg atacgccgcg cgtaatcgga tgcaatcggg tgccggaacc
     8821 gcccgccagg ataatacctt tcatgtttgc ctccgctgaa aattagcctt tcagacctaa
     8881 acgctcgccc tgatagctgc cgtcctgcac ctgcttccac cagctttcat tagccagata
     8941 ccactgcacc gttttacgca ttccactttc aaaggtttcc tgtggcagcc aaccaagttc
     9001 acgggcaatt ttcgaagcat caatggcata acgcagatca tgccccggac ggtcagcgac
     9061 aaaggtgatc aagtcacgat agtgcaccac gccgtgcggc ttgttcggag ccagttcttc
     9121 cagcagctcg caaatggttt ccacaacatc gagattctta cgctcgttgt gaccaccaat
     9181 attataggtt tcaccgactt tcccggtggt cgccacgcaa tacagcgcgc gggcgtgatc
     9241 ttccacatac agccagtcac ggatttgctg cccgttgcca tataccggca gcggtttacc
     9301 cgccagcgcg ttgaggatca tcagcgggat cagtttttcc ggaaagtggt aaggaccgta
     9361 gttattcgag cagttggtga tcagcgtcgg cagaccgtag gtccgcagcc aggcgcgcac
     9421 cagatggtcg ctgctggctt ttgacgcgga ataagggctg ctcggcgcat acggcgtggt
     9481 ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat acttcgtcgg tggagatatg
     9541 atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta ttccagtaag cccgcgccgc
     9601 ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata aacgctgccg ggccgtcaat
     9661 agaacggtca acatggcttt cggctgccag atgcatgaca cagtctggct gatgctcagt
     9721 gaatacgcgt gccagttctg cccgatcgca gatatcaact ttctcaaagg caaagtgctc
     9781 gctttgcgcg accggtgcca gcgacatcag gtttccggcg taggtcagct tatcgaccac
     9841 taccaccgca tcgctcgttt cgttgatgat ataacgcacc agcgccgagc caataaaccc
     9901 ggcaccacct gttatcagaa tttttctcat cagcgccaga ctcctttggc atcgacgaca
     9961 tactgctgat ggacattgtc gccattgata actttgaact gactatgatc gaccagcatc
    10021 accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg ccagagtaca aagcccggtc
    10081 agttttttcg gcaactggtg gatgttaggc tcaacaacca gcgtttcgcc gctgtgccac
    10141 tgggcgatca gttcagcgat ttccatcgcc gggctttcgc gcaggtcatc aatattcggt
    10201 ttaaacgcca gaccaaagca ggcgattttc agttcactgg cgcgtttatc ggtagcagca
    10261 aggcagtcgg caaccgccgc tttcacctga tcgataaccc agaacggttt gtgatcgtta
    10321 acttcgcgcg cggtacggat aagccgcgcc tgctgggggt tctgtgccac gataaaccac
    10381 ggatcaacag caatgcagtg accgcccacg ccagggccag gctgaagaat attaacgcga
    10441 gggtgacgat tcgccaggcg aatcagttcc cagacgttaa tcccctgatc ggcacaaatc
    10501 agcgacaatt cattagcaaa agcgatattc acatcgcgga agctgttttc ggtgagctta
    10561 cacatttccg ccgtccgcga gttagtgacg acacactcac cttcgaggaa aattttgtac
    10621 agttcgctgg cgcgggtcga acaaaccggc gtcataccac caatcacgcg atcgttttta
    10681 atcagctcga ccataacctg gcccggtaac acgcgttccg ggcagtaagc aatgttgacg
    10741 tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg gacgcatctc tgctaaccat
    10801 tctgccatct tctcggttga ccccaccggc gaggtggatt caaggatcac cagcgcgcct
    10861 tttttcagca ctggcgcaat ggagcgagca gccgattcaa cgtaggtcat atctggctca
    10921 tgatcgcctt taaacggcgt gggtacagca atcagccagg catccgcttc aactggcgtc
    10981 gtgctcgctc gtaaaaaacc gccttctacg gcagttttta ctacgctcgc caaatcaggc
    11041 tcgacgatat ggatttcgcc acgattgatg gtatcaaccg cgtgttggtt gatatcgaca
    11101 ccaatcacct gtttttgccg tgaggcaaac gcagctgctg taggcagccc gatgtaaccc
    11161 agtccgataa cagaaatggt cgcaaaactc atagtgatat ccgattattt tttaacgctt
    11221 ccagaatgcg agagcatgcc tgaccatcac catacgggtt atgggcgcgg ctcatagctt
    11281 gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc gacaattcgc tgcttatccg
    11341 tgcctaccag acgcaccgta cccgccgtca ccgcttccgg acgctcagtg gtatcgcgca
    11401 tcaccagcac cggtttcccc agcgaaggcg cttcttcctg aatgccgcct gagtcggtca
    11461 aaatcagcca ggcgtggttc atcagccaga caaacggtaa atactcctgg ggatcgatca
    11521 gaatgacatt tttcacatgc cccagaatgc gattgaccgg ctctctgacg ttcgggttga
    11581 gatgcatcgg atagacaatc tggatgtcct ggtgcgtggt ggcgatgtct gccagcgcat
    11641 ggcagatttc ttcaaagcca cgaccgaagc tctcacgcct gtgaccggtc accagaatca
    11701 tctttttatc ggggtcgata aacgggtaat ttgccgccag ttctgaacgc agcgtatcgc
    11761 tgctcatcac ctggtcacgc acccataaca gtgcatcgat gacagtatta ccagtaatga
    11821 agattcggct atccgcaacg ttttcacgca gcaagttttg ccgggaagtt tcggttggag
    11881 agaagtgata catcgccaga tgcccggtca atgtacggtt agcctcttcc ggccacggcg
    11941 aatagagatc gcccgtgcgc agaccagcct caacgtgacc aacaggaata cgctgataaa
    12001 acgccgccag gctggttgcc agcgtcgtcg tcgtatcgcc gtgaaccagc acgacgtctg
    12061 gtttgaactc ggcaagaata ggttttagcc cttccagaat ccgacaggtt atctctgtca
    12121 ggccctgtcc tggctgcatt atgttgagat cgtagtcagg tacaatggaa aagagtttca
    12181 gcacctgatc gagcatctcc cgatgctgcg cagtgacgca aactttagcc tcaaaaaaag
    12241 gatcttttgc caacgcatgc accaacggcg ccatcttgat ggcttccggg cgcgtaccaa
    12301 atacagtcag tactttcaca tcgattctct tcgaataagc ggcgagcgcc tttgcgctca
    12361 ccgcagcagt gttgctattt cgagcaacgg cgggttaatg cgacaccagc cccgatcagc
    12421 cccccgacaa tgccccacat aatcatcagg aaggcacgac gtgggctatc gcgttttacc
    12481 ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac gcggatccag ggttggacca
    12541 acattcaggg tgtttaacat ggcccgattc tgatcatagt cgagatcaaa ggccggaccg
    12601 acggcctgta aattttccag tcgagcctga agcattggac gcccaagcag gaacatttct
    12661 gaatcaggta attcctcggc aggcacatct gtcgcactgc gcgaaatatt atgctgctca
    12721 gcaattttca gcgcctgctc aatgctgttc atccggcggt cgtagatggc tttcgccacc
    12781 tcttcctgac gcttcacctg agctttcatc tggatggtac gcgccgccca tgcgcctttc
    12841 agctcatcat tcagatggct ggctgcacgc tggctggcaa aagcaacata ctgacgtaac
    12901 aggttattag cgtcaggcgc ggtttcggca ataagcttca cgctgtcatt gaccgcgcgg
    12961 gtaaagtctc cggggataaa ctggatgttg ttaatcattt catccagcaa cgccgcatcg
    13021 gctttgctgt tgcccaccat ccgctgtttg taatagtcgg tttgcagcca gaactctctg
    13081 cgggtatccc acgaggccag ttgcataaca aactctttat aggcttcgtc catgaccgat
    13141 ggttggtcgg cagaagccat gtttgaacgg acatccaggt tacgcaaaaa ttgctgctgc
    13201 gagtaatatc cccccagcat attcaccgtt ggacgatcgg taatcgccgt cgagctccac
    13261 tcctgacgag caaaaaacgt atacgccagc gcgattaacg caaacgccag ccccatgcca
    13321 ataatccata gcttcccagc ccacaaggta cgaaacaacc cacgaatatc cagttcattt
    13381 tcagcgtctt cggccggttt cccaggcatt ggttgtgtca tcacatcctc atttatttgg
    13441 ttaaattggg gctgccacca cgatttctac gcagtctgcg ttttacgcgc ttaataaagc
    13501 gagcaacttt ccaggcacgc ttaatgcaat atccatagag gaagaatgct agcaaaaaga
    13561 gcaccagcat gacccactcc gggacaaaat gagaatattc tgccagcacg ccaatggaag
    13621 cgagcagtgc cgcagcaaga gtaatcagca caaacgcctg gcgggaagta aacccggcac
    13681 gcatgatcaa atggtgaata tgctgacggt caggagagaa tgggctcatg cctttacgca
    13741 ggcgacggta cataatcgcc accatatcca ttagcggaat ggcgattatc cacaaagcgg
    13801 taaccgggct gatgggatgg gttttaccct gggtcgtttc gagcaggatc cagatcacgg
    13861 taaaaccaat cagcgtactg cccgcatcac ccataaagac tttgtagcgg cgacccagga
    13921 taccaaggtt aagcatgatg tatggcagga tggcggcgat catcgcaaag caccagattg
    13981 cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat tgctgcaaac gagacgcagg
    14041 acaacccgcc cagcaagcca tcaatgccat caaccatgtt gaacgcatta atggccgccc
    14101 agacggcaaa tagcgtcagg aagtaaccaa acggtccgag caccatctcc caggagccaa
    14161 agatataacc caggctactg agataaagtt tgccgaacac catcataaca atgccaacag
    14221 cggcctgtat ggtggcacgg atttttacgc tgatatcaaa acggtcatcc agcgcgccaa
    14281 tgaaaacaag cacaccggca caagcgagat agagagatgc atgcggaata tagtaatcga
    14341 caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat ccccccaacg agaggtatca
    14401 atccctggtg acgtttgcgg aagtttggtt tatccactaa accgactttt tttgccacct
    14461 tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat actgatgaga tcagtactca
    14521 ctgtcagtaa attcacaatg catgctctca gagaaaatta ttagcagaag tataaccacg
    14581 aagaccttta ttcagaaggg aaagtccgtt ccgaacccac cagtcctgtg caaaaattaa
    14641 ccatttgata tgcaaattgc ataacatgcc agcaaattcc aggaattttc ctaacatcgc
    14701 cagcgcggca ttaagattac agatataaaa gcaaaacgcc acgtaaacac gtggcgtttt
    14761 tggcataaga caaatttatg agcgtttcat catttcgaag aaatcgtcat tggtcttggt
    14821 catggccagt ttattaatga ggaattccat tgcatcgatt tcgcccatcg ggtgaatgat
    14881 tttgcgcagg atccacattt tctgcagttc ttcctgagtc gtgagcagct cttctttacg
    14941 ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt ttttcagcga tcttacgaga
    15001 gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg taaataactt cgtccatttt
    15061 agaaccggta tcgataagcg ccgtcgcgat aatggtcagg ctgccgccct cttccacgtt
    15121 acgcgccgca ccgaagaagc gtttcggacg atgaagggca ttggcgtcca caccaccggt
    15181 caacacttta cctgacgccg gaacaacagt gttgtaagcg cgcgccagac gagtgatgga
    15241 gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg cgtttggcct tctcgatcac
    15301 catttccgca acctgaacgt ggcgagatgc gggttcgtca aaggtagaag caacaacttc
    15361 accttttacc agacgctgca tctcggttac ttcttccgga cgttcgtcga tcagcagaac
    15421 catcagcaca caatccgggt ggttgtaagc aatgctctga gcaatgttct gcagcagcat
    15481 agttttaccg gctttcggcg gtgccacaat cagaccacgc tgaccacgac cgataggtga
    15541 tgccagatcc agtacgcgag cggttaaatc ttccgtagaa ccgttaccac gttccatacg
    15601 cagacgagag tttgcgtgca gcggggttaa gttctcaaag aggattttgt tgcgggcgtt
    15661 ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc gcaaaatagc gttcaccttc
    15721 tttcggcggg cgaatcttac cagagatggt atcaccagtg cggaggttga aacggcggat
    15781 ttggctaggg gaaacgtaga tgtcatcagg accggcgagg taggagctgt ctgcggaacg
    15841 gaggaaacca aatccatcct gcaatatctc cagtacgcca tcaccaaaga tatcttcgcc
    15901 actctttgcg tgctgcttca ggatggcaaa aataatgtcc tgcttacgca tgcgagccag
    15961 gttttccagc cccatatttt cgccgagagt gatcagctca gaaaccggcg tattctttaa
    16021 ttcggtaaga ttcatagtgg tgtgagttct taaacttggg gtaaatctcg aacttaatgt
    16081 tgtgaatggt atggcaggat catccatgcc tgtttacggt catcgactca tgtcttttcg
    16141 ctgcctggtc acaggagagt acgcagaact gaaacgacaa gacggattga gtgacgagcc
    16201 agaaatctgt tcacttcgca tttaagataa aacgggaagc gttggaaatt acaagattca
    16261 aacttaataa ggtatgttta atacgaagtc aacactaagt tagcatgact cacgccgggc
    16321 gtccagtttt tagcgacggg gcacccgaac atgaaattcc cttacgccag gttagcgtcg
    16381 aggaactctt tcaactgacc tttagacagt gcgcccactt tggttgccgc cacttcaccg
    16441 tttttgaaca gcagcagagt cgggatacca cggatgccat atttcggcgc agtgccaggg
    16501 ttttgatcga tgttcagttt tgcaacggtc agtttgccct gatattcgtc agcgatttca
    16561 tccagaatcg gggcgatcat tttgcacgga ccgcaccact ctgcccagaa atcgacgagg
    16621 atcgccccgt ccgctttgag tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt
    16681 ttatcgctca tatataactc cacaggaata agcctggcgt gttggtttcg ttgttggtgt
    16741 aacattaacc aactaaaggt tgactttatt tcaccggata cgctttcgta aagcaatagt
    16801 aagctgatat tctaccacac tatgagcaaa acacatttaa cagaacagaa gttttccgac
    16861 ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa aagggtttca taactgtacg
    16921 cccattcagg cactggccct tccgctgacg ctggcgggtc gtgacgtagc cgggcaggcg
    16981 caaactggta ccgggaaaac gatggcgttt cttacgtcaa cgtttcatta tcttctctct
    17041 catcctgcga ttgccgatcg caaggtgaat caaccgcgtg ccttaattat ggcaccgacg
    17101 cgtgaacttg ccgtgcagat ccatgccgac gcagaaccgc tggcggaagc tactggcctg
    17161 aagctgggtc tggcttacgg tggtgatggc tacgacaaac agctgaaagt gctggaaagc
    17221 ggcgttgaca ttctgattgg caccacgggg cgtttaattg actacgccaa gcagaaccac
    17281 attaacctcg gtgccattca ggtggtggta ctggacgaag ccgatcgcat gtacgatctg
    17341 ggctttatta aagatatccg ctggctgttc cgccgtatgc cgcctgcaaa ccagcgcctc
    17401 aacatgctgt tctccgccac gctttcgtac cgggtacgtg aactggcgtt cgagcagatg
    17461 aacaatgccg aatatattga agtggaaccg gaacagaaaa cgggccaccg tattaaagaa
    17521 gagcttttct acccttctaa cgaagaaaaa atgcgtttgc tgcaaacgct gatcgaagaa
    17581 gagtggccag acagagcgat tattttcgcc aacaccaaac accgttgtga agagatctgg
    17641 ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga caggcgatgt cgcgcagaaa
    17701 aaacgtctgc gtattcttga tgaatttacc cgtggcgatc tggatattct ggttgccacc
    17761 gacgttgccg cgcgtggttt gcatattccg gcagtgacgc acgtctttaa ctacgattta
    17821 cccgatgact gtgaagatta cgttcaccgt attggtcgta caggtcgcgc aggcgcaagc
    17881 ggtcactcta ttagcctggc gtgtgaagag tatgcattga atttgcctgc tattgagacc
    17941 tatattggtc actcaattcc ggtaagcaaa tacaatccgg acgcattgat gaccgatctg
    18001 ccaaaaccgc tgcgcctcac gcgcccgcgc acaggcaatg gtccgcgtcg tactggcgct
    18061 ccgcgtaatc gtcgtcgttc aggttaaata aaaaatgtca tactcaaaat cattcaaact
    18121 gtatcaagat ggcaagagaa tgaatcccga tgagcttact gtagtaagtg attcaggtga
    18181 aagaatgcag ccaacacaga gacagattga aggatgaaga gtatgggttc cacctcgtcg
    18241 ctgtatgcag ccattgatct cggttcgaat agttttcata tgctggttgt gcgcgaggtg
    18301 gctggaagca tccagacgct gacgcgaata aaacgcaaag tgcgtctggc tgctggcctg
    18361 aatagtgaaa atgccctctc taatgaagca atggagcgcg gttggcaatg tctgcgcctg
    18421 tttgctgaac gtctgcaaga tattcctccc tcgcaaattc gcgttgtcgc tacggcgacg
    18481 ttacgccttg ccgtcaatgc gggtgatttt attgccaaag cgcaggaaat cctcggttgt
    18541 ccggtacagg tgatcagcgg tgaagaggaa gcacgtctga tttatcaggg tgttgctcac
    18601 accactggcg gtgccgatca gcgcctggta gtggatatcg gcggtgccag tactgaactg
    18661 gtaaccggca cgggtgcaca aactacctcg ttgtttagcc tgtcgatggg ctgcgtcacc
    18721 tggctggaac gctattttgc cgatcgtaat ctggggcagg aaaattttga tgctgcagaa
    18781 aaagcggcac gcgaagtgtt acgtccggtt gccgatgaat tacggtatca cggctggaaa
    18841 gtgtgcgttg gtgcttccgg caccgtgcag gcgttacagg aaatcatgat ggcacagggg
    18901 atggatgaac gcattaccct ggaaaagttg cagcaattga aacagcgagc cattcattgc
    18961 ggtcggctgg aggaactgga gattgacggg ctgacgctgg aacgtgcgtt agtgttcccg
    19021 agtggtctgg cgatcctgat cgccattttt accgaactga atattcagtg tatgaccctg
    19081 gcgggcggtg cgctgcgtga aggcttggtc tacggcatgt tgcatctggc cgtcgagcag
    19141 gatattcgca gccgtacgct gcgtaatatt caacgccgct ttatgatcga tattgatcag
    19201 gcacagcgcg tagccaaagt tgcggctaac ttcttcgatc aggtggaaaa cgaatggcat
    19261 cttgaagcaa taagccgcga tttgctcatc agcgcctgtc agcttcatga aatcggcctg
    19321 agcgttgact tcaaacaagc gccgcaacac gctgcttatc tggtgcgtaa tctggatctt
    19381 cccggtttta cccccgcaca gaaaaaattg ctggcgacgc tactgctcaa ccagactaat
    19441 ccggtcgatc tctcatcgct gcatcagcaa aatgccgtac cgccgcgcgt cgcagaacaa
    19501 ctctgccgtt tactccgcct ggccatcatt tttgccagcc gtcgccgtga cgatctcgtg
    19561 ccagagatga cattacaggc taaccatgaa ctgttgacct tgacgctacc gcaaggttgg
    19621 ctaacccaac atccgctggg taaagagatt attgctcagg aaagccagtg gcagagctat
    19681 gtccactggc cgctggaagt gcattaatga gtaagtgccg gatgcgatgc tgacgcatct
    19741 tttacggcct tgattatttc cctcgttttg ccgccatcat cgctttcaga ttcgccagat
    19801 ggctttgccc tttctgcatc cgttcttcgg cgctgaccac tttgcgctcc tgttcccaaa
    19861 tcagatcatc ctgcggcagc tccagcaaaa agcggctcgg ctccgggcgc accagttcgc
    19921 cgtactgacg gcgttcttta cacagcgtaa aggtcaattc cttctgggcg cgggtaatgc
    19981 cgacataggc cagccgccgc tcctcatcga tattatcttc atcgatactg ctctgatgcg
    20041 gcaaaaaccc ttcttccata ccgaccatgt agacataagg aaactccagc cctttcgacg
    20101 cgtggagagt catcagttgc acctgatcca gctcttcttc actctcacca cgctccatca
    20161 tgtcgcgcaa agtaaagcgc gtcaccacct gggtgagcgt catcggctca tccagttcgc
    20221 tgccttccag catctccgtc atccagctaa acagttggtt gacgttcttc atgcgcattt
    20281 cggctgcttt cgggctgggc gatgtttcgt acagccagga ttcataatcc atgccatgga
    20341 tcagatcacg caccgcagca atcggctccc gctccgccag gcgctggatt tctgccaacc
    20401 agtgagtgaa gcgggtcaat gcttcataac cacgtccgct aagcgtctga ctcaggccca
    20461 tatcaaagct ggcggtaaac atgcttttat tgcgcgtcat cgcccactca cccagctttt
    20521 tcagcgtcgc cgggccaatc tctcgcttcg gcgtgttaac gatacgcaga aatgcgctgt
    20581 catcgtccgg gttagtcagc acgcgcagat aagccagcaa gtccttgatt tcaggacgag
    20641 agaaaaacga cgtaccacca gaaattttgt acgggatgcg gttttgcatc aggaattttt
    20701 caaacacccg cgactgatgg ttaccgcgat aaagaatggc gtaatctttg tactgcgttt
    20761 tattgacgaa gtgatgggcg atcagctcgc cagtaacgcg ctcggcttca tgttcctcgt
    20821 tattcgcgct taaaactttt agctctgtgc cgtaacccag ttcggagaac agacgctttt
    20881 caaagacgtg cggattattg gcgatcagga tgttcgccgc tttcaaaata cgtccggaag
    20941 agcggtagtt ctgctcaagc ttaatcacct tcagcgccgg aaaatcctga ctcagcagca
    21001 ccaggttttg tggacgtgcg ccgcgccagg agtagatcga ctggtcgtca tcgcccacca
    21061 cggtaaagcg cgcgcggctg cccaccagca gtttcaccag ctcatactgg ctggtgttgg
    21121 tgtcctgata ctcatccacc agcagatagc gaattttgtt ctgccagcgc tcgcggactt
    21181 cttcattgcg ttgcagcagc aacgtcggca ataaaatcag atcatcgaag tcgagaacgt
    21241 tacaggcttt caggtgtgca tcatacagcc cataacaatg ggcaaaaata cggtcccgct
    21301 cgccaatcgc acttgctgcc gcctgggacg gtgttttgag atcattcttc cagttagaga
    21361 tggtcgaaat cagttgttgc aggagaactt tgtcatcttc aatcagcccc tcggtcaact
    21421 ctttaagcaa agcaagctga tcggtatcgt caaacaacga gaagttcgct ttcatcccaa
    21481 gcgccgcata ctcgcgtttg atgatatcca accccagcgt gtgaaaagtg gagatcatca
    21541 gcccgcgcgc ctctttgcga cccagcgtct gcccgacacg ctctttcatc tcgcgcgccg
    21601 ccttattggt aaaggtcacc gccgcaatat gtcgcgcctg ataaccgcaa ccgcggatca
    21661 gatgggcgat tttattggtg atcacacgag ttttaccgga acccgcgccc gccagcacca
    21721 ggcaggggcc ggtaacgaat tcgacagctt gttgttggcc ggggtttaga cgcataggtg
    21781 tattgctcaa tcttcgaacg gggggaggat tgtagcagaa tcgcatttga atgtgaccgc
    21841 gtgtaagccg ctaaccggtt ggctgcggat gtttaaatca gagagagaag accgaaatcg
    21901 gagttcatcg tgaatccatt agacttagaa aatatcgggt cgtaacgttg tgttattact
    21961 ttttcagagt ttactaagga gaggtttact cgtggcgatt aatttcagcc ctaaggttgg
    22021 tgaaatactg gaatgcaact ttggtaacta tcctgttagc caaaatggac cgttcagcac
    22081 gacgtattac gatggccgca ttcctcctga aatgataaaa aacaggttag ttgtcgtgct
    22141 caacggaaaa attaatggca acgcatgcat cgtcgttcca ttatcaacca ctcgcgacca
    22201 tgacaagtta aaacgtggaa tgcatgttga aatagccagt aatgttatca atgacctgca
    22261 attctttgat cagcagattc gttgggccaa agccgacctg gtacaacaag tcagtcgaaa
    22321 cagattaaac agagcaagaa cttacagagg gtatcttaat caatgtttgc cacatgagtt
    22381 ggttgcagac atacagaggg cggtcatcaa gtcaatcaat gccatatcgc tcataaattg
    22441 acaataaaat caccatcccc gataatgact ttgtagccca tagaggccct aagttttctc
    22501 cccttgaggg acctgcgaag cccggatttt ccgggctttt gccatatatg taccagcccc
    22561 ttcctgactg acaattacgt tagcatctgc ggatcatgct aaaatcgccg cctgacaatt
    22621 attcactcaa ggcacgatca tggcaaaaac agcagcagca ctgcatatcc ttgtaaaaga
    22681 agagaaactg gctctggatc ttctcgagca gattaagaac ggtgccgatt tcggcaagct
    22741 ggcgaagaaa cactccattt gcccatcagg caaacgcggc ggtgatttag gtgaattccg
    22801 ccagggtcag atggttccgg cgttcgataa agttgtgttc tcttgtccgg tactggagcc
    22861 aaccggcccg ctgcacaccc agttcggtta ccacatcatt aaggtgctgt accgtaacta
    22921 atagcaaggc cttctccagg agaaggcctt gagtgttttc tccctctccc tgtgggagag
    22981 ggtcggggtg agggcatcag cgcgcactta acccgcaact gcaatacgtt tcatatccgt
    23041 catatagccg cgcagtttct tacctacctg ctcaatcgca tggcagcgaa tcgcttcgtt
    23101 cacatcacgc agttgcgcgt tatctaccgc accttccgga atagctttac ccagatcgcc
    23161 cggttgcagc tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata
    23221 gttaccgtac tcggcggtat cagagataac cacgttcatt tcgtacagac gcttacgggc
    23281 gatggtgttg gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat
    23341 gatgccggaa tcaaccatgg tttcaaacgc cagctcaacg cccgctttca ccatcgcgat
    23401 catcagtacg cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc
    23461 ggtttcgaag gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt
    23521 ggcccagtcc gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt
    23581 ctggaacagg ggtgccatga tctctttcag ctgttcagac agcgcgtaag cacgcagttt
    23641 cgccgggtta gagagacggt ccatcatcag ggtgatgccg ccctgcttca gcgcttcggt
    23701 gatggtttcc caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc
    23761 caccagcttg tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg
    23821 ctcacccatc aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg
    23881 gtgaccaccg gttgctgccg cccatgcttt agcgatcgcc ataccttcgc ctttcggatc
    23941 gttttccggg tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg
    24001 cacttcggtg ccagggcatt tcggcgcaac catgactacg gtgatatctt tacggatctg
    24061 ctcgcccact tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat
    24121 cagtggctgt acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac
    24181 cagatccgcc tgcgggatca gttcttcgta agtaccaact ttaaaaccgt tttcggtcgc
    24241 tttacgccag gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc
    24301 gagaccagaa tcacgcatgt tcaggccctg gttcagcccc tgtgcgccac agccgacgat
    24361 aaccactttt ttaccctgaa ggtagctcgc gccgtcggcg aattcgtcgc gccccataaa
    24421 gcgacatttg cccagctgtg ccagctgctg gcgcaggttc agtgtattga agtagttagc
    24481 catggtgatt cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa
    24541 ttcactatat gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa
    24601 tttctgcaac gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc
    24661 tgcatctggc ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt
    24721 ccacgctctc acggcagatt cagcgcctgg aagacgatct cggtcaggca ttatttgtgc
    24781 gcgataaccg gacggtgacg ctgaccgaag cgggcgaaga gttacgcgtt ttcgcccagc
    24841 aaacattgtt gcagtatcag caactgcgcc acaccatcga tcagcaaggg ccgtcgctct
    24901 ctggcgagtt acatattttc tgctcggtga ccgccgctta cagccatcta ccgccgattc
    24961 tcgaccgctt ccgcgcggaa cacccgtcgg tggagattaa acttactact ggtgatgcgg
    25021 cagatgcgat ggaaaaggtg gtcactggtg aagcggatct ggcgattgcg ggtaaaccgg
    25081 aaaccttgcc cggcgcagtg gcgttttcga tgctggagaa tctggcagta gtgctgattg
    25141 cccccgcgct gccctgcccg gtgcgtaatc aggtgtcggt agagaagccg gactggtcaa
    25201 cggtgccgtt tatcatggcc gatcaggggc cggtacgccg ccgcattgaa ctgtggtttc
    25261 ggcgtaacaa aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg
    25321 tatcgatggt ggcgctcggc tgtggcgtgg cgctgttgcc ggaagtggtg ctggaaaaca
    25381 gccccgaacc ggtgcgtaac cgcgtgatga tactagagcg aagcgatgag aaaacgccgt
    25441 ttgagctggg cgtgtgtgcg cagaaaaagc ggctgcatga gccgctgatt gaagcgttct
    25501 ggaagatttt accgaaccac taacccgcca aaaagaacct gaacgccggg ttattggttt
    25561 cgtcgtggca atcgtagccc agttcattca gccgggtttc gaaatccggt tcatggtcgc
    25621 caagctcgaa cgccgccagt acgcgcccgt agtcggtacc gtgactgcga taatggaaca
    25681 gagaaatatt ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgca cccggcgatt
    25741 ccggaaattc gaagctgtag agacgttcct gcaacggatg cgatggacgc ccgccaacca
    25801 tatagcgcac atgtagcttc gccatttcat cgtcggagag atcaaccacg ctgtagccgc
    25861 cgtcgttgag catctgcaaa atttctttgc gctcttcgag gccgcggctt aagcgcacgc
    25921 cgacaaagat gcaggcgttt ttggcatcgg caaagcggta gttgaattcg gtgaccgaac
    25981 gtccgccaag caactggcag aatttgagga agctaccttt ctcttccgga atggttaccg
    26041 ccagcaacgc ttcacgctgt tcacccagct cacaacgttc cgagacgtag cgcaggccgt
    26101 gaaagttcac gttagcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct
    26161 gggcgatata ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac
    26221 gcacatcttc gaataaatcc ttcatcgccg cacagatcgc atcactatcg acggtgatga
    26281 tatcgtcgag atactcctgg cataaacgga aggtttcgtc gccgatgcgt tttaccgcaa
    26341 cgccttcagc aaacagcccc acgcgcagca gatcaaccgg atgacccgca tccagcgctg
    26401 ctttcaggca ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt
    26461 gtttgatcag caccgccacg ccagcagcca gaccgccgcc gccgactggc acaaatacgc
    26521 ggtcgagatg ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca
    26581 ccatcggatg gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg
    26641 ctttggcttt cgcttcatca aagttcgcgc cgtggagcag cacttcgccg ccgaagccgc
    26701 gtaccgcatc aactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc
    26761 ctaaccgcgc agaagaaaac gcgacgccct gcgcgtggtt acccgccgag gcggtgatca
    26821 cgccgtgcgc tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct
    26881 taaagctgtg cactggctgg cgatcttcgc gtttcaccag aatcacgtta tcaagacgag
    26941 acgagagctt ttccattttt tgtagcggcg tgacctgcgc cgcttcgtag accggcgcgc
    27001 gcagcaccgc tcttaaatat tcagcacctt ccggggcacc ggacaggggt tgcgagtcag
    27061 ccattattaa ccccccagtt tcgatttatc gcgcaccgcg cctttgtcgg cgctggttgc
    27121 caggctggca taagcacgca gggcaaagga gacctgacgt tcacgatttt tcggcgtcca
    27181 ggctttgtcg ccacgggctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac
    27241 ctgtaactga atgccacggt ttggaatgtc gatggcgatc aggtcgccgt cttcaatcag
    27301 gccaatgctg ccgccgcttg ccgcttccgg tgagacgtgg ccgatagaaa ggccagaggt
    27361 gccgccagag aaacgaccgt cggtgatcag cgcacaggct ttaccgagcc ccattgattt
    27421 caggaagctg gttgggtaga gcatttcctg catccccgga ccgcctttcg ggccttcgta
    27481 gcggataaca accacatcgc ccgcgacaac tttaccgccg agaatcgctt ctaccgcgtc
    27541 gtcctggctt tcgtatactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc
    27601 cgcagtttta acgatgcagc cgttttccgc gaaatttccg tagagcaccg ccaggccacc
    27661 gtctttgctg taggcgtgtt ccagcgagcg aatacagcca tttgcgcgat cgtcgtccag
    27721 cgtatcccag cggcaatcct gcgagaatgc ctgtgtggta cgaatgcctg cagggcctgc
    27781 gcggaacata ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt
    27841 ttgcggcaac gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc
    27901 cagttcaccg agaataccga taacaccacc agcacggtga acatcttcca tatggtattt
    27961 ctgggtgctc ggcgcaactt tacacagctg tggaaccttg cgggaaagct tatcgatatc
    28021 actcatggtg aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtacggtgtt
    28081 agtcgatcca cccatcgcga tatccagcgt catggcgttt tcaaacgccg ccttactggc
    28141 gatattacgc ggcagtgcac tttcgtcgtt ttgctcgtaa taacgtttgg tcaattcaac
    28201 aatgcgttta ccagcattaa ggaacagctg cttacggtcc gcgtgggttg ccagcagcga
    28261 gccgttgcct ggctgcgaca aacccagcgc ttcggtcagg cagttcattg agttagcggt
    28321 aaacatcccg gagcaggaac cgcaggttgg gcaggcggaa cgttcaacct gatcgctctg
    28381 ggagtcagaa actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagcttgat
    28441 gatctgctcg gacagtttgg ttttcccggc ttccatcggg ccgccggaaa caaagatcac
    28501 cggaatattc aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga
    28561 gatgcagacc atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat
    28621 cagttcgcga gatggcagtg aataaagcat ccccccgtgg cccatagcaa tcccatcatc
    28681 caccgcaatg gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac
    28741 cagtttaccg agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac
    28801 aaccgcgata atcggcttgc caaaatcggc atcggtcatt ccggtggcgc gccacagcgc
    28861 acgagcaccc gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact
    28921 ttatttactc ccagtgtctg tctcgtaaat gggacggtga gtgccgtccc attttttgta
    28981 tttattgatt aacttgatct aaccagcccc atttatcttc ggtttcgccg gtaaagaggc
    29041 cgaagaaggc ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaacctgaa
    29101 taccgtctac gctgcgcact ggcgtgattt ctgccgctgt cccagacata aacacttcat
    29161 ccgccaggta cagtgattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt
    29221 tcgccagttt gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg
    29281 gggtgaacag cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat
    29341 aaccgttcac atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca
    29401 ccagcaggga agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg
    29461 ctgcgcggtt ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca
    29521 gatacgcccc ccacgggaaa gcggcgataa tcacgtcggt tgagtatccc gctggcgggt
    29581 ttacgcccat gccaacatca ccaacgaaga tcagcggacg gatataggcg ctggtgagat
    29641 tgtttttgcg gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgcgaaaccg
    29701 ggaagcgata gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata
    29761 caaccggtcc tttgtgcgag tcgtagcaac ggatgccttc aaaaaccgag gttccatagt
    29821 gcagcgcgtg cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga
    29881 accaaatgta atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg
    29941 atttgttgtg atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta
    30001 tttaactgac taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt
    30061 atattttgtg catcgctggc ggcggccata ttcattgagc agacgtggaa accacgatga
    30121 cgcaccacgc gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc
    30181 tgatgttgca tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca
    30241 gcggccagac gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt
    30301 tcagcatggt gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt tggccaggga
    30361 tgccgaaggc gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt
    30421 atcgttcctg gaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga
    30481 gtaagacgat tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca
    30541 tcatgaaaga gccgtcaccg gagatacaga cgacggtatc gttcggtcgc gcgacttgcg
    30601 cgccaaccgc cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat
    30661 tttccgggcg agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg
    30721 tggtcacgac gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggcgcgt
    30781 agatagcgtc accgggatgg tcgtaacgcc aggcatgttc atcacgcagc tgcgcgcagt
    30841 gttgctgcca gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat
    30901 caccttgtaa tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca
    30961 tatggataac actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa
    31021 aacgtgcacc cacggcgatc agcaagtcgc actcctgcac cgcgaagttc gccgctttgg
    31081 tgccatgcat tcccagcatg cccagatagt acggataatc tgcttcaact gcgcccagcc
    31141 ctttcagcgt gcaggtggca ggcatttttg tggtagcgag aaattctcgt aaagcaggaa
    31201 ctgcctgcgc catacccacg ccaccaccaa cgtacagcat cggtttttgc gcttttgcca
    31261 gcatctggcg cgcttgctca acttcggcat gggggaaagt cacttcgttt tcaacggtgg
    31321 tgaaccacgg ttccagatcg ccgctggcta actggatatc ttttgggata tcgaccagaa
    31381 ccggaccagg acgacctgag ctggcaactt cgaatgctgc agccatgatg cgcggcaact
    31441 cttccagcga ctgcaccagg aagctgtgtt tggtacaggc taacgacaat cccaggacat
    31501 ccacttcctg aaatgcgtca gtgccgataa acggtgcgga cacttgaccg gtgatggcaa
    31561 caacggggat ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg
    31621 gaccagacgt ggcgatacat acgccagttt tgccagtagc acgggcataa ccgatagccg
    31681 ccattgccgc accctgttca tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg
    31741 catcgtaaac cggcataatt gcgccacccg gataaccgaa aacggtattc acaccctgtg
    31801 cccgcaacgc atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct
    31861 tgagaaacag aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc
    31921 aaaaaaaacc cccggacctt tcggtgcggg ggtcttagtt cgttaaggct tgatttctaa
    31981 gcctttcctc gtccaagtgc agccccgcac ggtgggataa taatcaccac cacgctaatc
    32041 acgaccaggc taatcactcg tagaagggct gtcattttgt cttttcttgc atcttgttcg
    32101 aaggaatgct taaagagtta ccataggttt tgtaaatagt acaagatatt ttttggccga
    32161 tttttatgat gcgtttaata atttcaatag aaattgttgt tttttaaaaa gaaaatagtg
    32221 agtgaaaaaa attcaatttc ggtctgaatg ttgttccctt cctcttagtt catcctttgt
    32281 ccgctgtatt tttgcgagca tcattccacc gccagaacaa ctcaacttca cttgcacaaa
    32341 taaacaggaa tcagccacta aaattcacga attagcgggc tacagcagcg atatcttatg
    32401 cacataatgg tgactcaagg agggcttatg tcactgtcaa ttgttcatac ccgcgcagcc
    32461 ctgggagtaa atgcgccccc aatcactgtt gaggtacata tcagtaaagg tctacccggc
    32521 ttaacgatgg tgggcttacc agaaacaacg gtgaaagaag ctcgcgatcg cgtgcgcagc
    32581 gccattatca atagcggata tgaatatccg gcgaaaaaaa tcaccatcaa ccttgctcca
    32641 gccgatctac caaaagaagg gggacgatat gatttaccta tcgccattgc gttgctggcg
    32701 gcctcagaac agcttacagc caataagtta gatgaatatg aattagtcgg agaactggcg
    32761 cttacaggcg ctctgcgtgg cgttcccggc gcaatctcca gtgcaactga agctattaag
    32821 tcgggcagaa aaattatcgt cgcgaaagat aacgaagatg aagtggggct aattaacggt
    32881 gaaggatgcc tgatagccga tcatctgcaa gctgtctgtg cgtttctgga aggtaagcac
    32941 gctctcgaac gcccgaaacc aactgatgca gtatcccggg cgctacaaca tgatctcagt
    33001 gatgttgtcg gtcaggaaca tggaaagcga ggactggaaa ttaccgccgc tggcgggcac
    33061 aaccttttac tgattgggcc tccgggaaca ggtaaaacaa tgctcgccag ccgtattaat
    33121 ggcctgttgc cagatttaag caatgaagag gcactggaga gtgctgcgat attaagtctg
    33181 gtaaatgctg aatcagtaca aaaacaatgg cggcagcgcc cgttccgctc acctcatcac
    33241 agtgcatcgt taactgcgat ggtaggcggt ggcgcaattc cagggcccgg tgaaatttcg
    33301 ctggcgcata acggcgtgct ttttcttgat gagttacctg aatttgaaag gcgtacactg
    33361 gatgccttgc gagagccgat tgaatccggg cagatccatc tttcacgcac gcgagcaaaa
    33421 ataacctatc cagcccgttt ccagcttgtc gcggcgatga atcccagccc taccggacat
    33481 tatcagggaa accataaccg ctgcacacca gagcaaacgc tgcgttatct caaccgactc
    33541 tctggtccct ttctcgaccg cttcgatctc tcactggaga tcccattacc gcctcccggt
    33601 attttgagta aaacggtagt gccgggagaa agcagcgcca ccgttaaaca acgcgtaatg
    33661 gccgccagag agcgccaatt taagcggcag aataaactga acgcctggct ggatagtccg
    33721 gaaatacgcc aattctgcaa acttgagagc gaagatgcga tgtggctgga agaaacgctg
    33781 atccatctgg ggttatcgat tcgtgcctgg cagcggttat tgaaagttgc acgaaccatt
    33841 gctgatattg atcagtctga cattatcaca cgtcaacatt tgcaggaggc agttagctat
    33901 cgagcgattg accgtttgct catccatctg cagaaactac tgacataaaa aaagggcatt
    33961 tcgccctttt tattaatcat cagaatcggt gtagtcttca gcaccttcaa cctgcggttt
    34021 accgccggaa agggtgtgaa aacgttttgg acgcttaata cgcgtcatat acttggacca
    34081 cacgcgttct gcttctgtca ctggctcacg ttcgccacgg catactgcta cgaagagttt
    34141 ctcttcctcg gtaaccggct cgcgtttgcc aagatccaac tcattgaagg cataaccatg
    34201 acgctcaagc agttgtgcct ctttgatggt gaaatcacca tgacgagaga atccacgtgg
    34261 ataatgttta ttgtcgaaat atcgattagt cgtcgtaaag ctttccgcca tcctgcacgc
    34321 tcctaattct ttgaccgagc tagttatggc gcggagtatt agttacgctt gacagagtgt
    34381 aaaacaaaac atttaaatca taacgacaaa taattttgcg gagagcactg tggatacgga
    34441 attgttaaaa actttcctgg aagttagccg aacgcgtcac tttggtcgag cggctgaatc
    34501 gctctatctg acccagtcag cagtgagctt tcgaatcaga caactggaaa atcaactggg
    34561 tgtgaacctt ttcacccgcc acagaaacaa tatccgttta accgctgccg gtgaaaaact
    34621 actgccttat gcagaaacgc tcatgagcac ctggcaggcc gcccgtaagg aggtggcgca
    34681 tacctcacga cataacgagt tttctatcgg tgccagcgcc tcgttgtggg aatgtatgct
    34741 taatcagtgg ctgggacgct tgtatcaaaa tcaggatgcc catacaggct tacagttcga
    34801 agcgcgaatt gcccaacggc agtctctggt aaaacagctg catgaacgcc agcttgatct
    34861 tcttattacc actgaagcgc ccaaaatgga cgaatttagt agtcagttgc tgggatattt
    34921 cactttagcg ctttatacca gtgccccttc aaaactaaag ggagatctta attatctgcg
    34981 acttgagtgg gggccagatt ttcaacagca tgaggcaggt ttgatcggtg ctgacgaagt
    35041 gcccattctg acaaccagtt ctgctgaact ggcacagcaa cagattgcga tgcttaatgg
    35101 ttgcacctgg ctacccgtca gctgggcgcg taaaaaaggc ggcctgcata ccgttgtcga
    35161 tagcacaaca ctttcacggc cgctttatgc catatggctg caaaatagcg ataaaaatgc
    35221 gttgattcgc gatcttttga aaattaacgt gctggatgaa gtgtattaat atgaatggct
    35281 ggcaaggatg ccggtagaag gatttacttc ggagagggtt atttcagata aaaaaaatcc
    35341 ttagctttcg ctaaggatga tttctggcag gggcggagag actcgaactc ccaacacccg
    35401 gttttggaga ccggtgctct accaattgaa ctacgcccct aattagggtg gcggaacgga
    35461 cgggactcga acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc
    35521 gctccaccga attcttttac aaccaccggt tttatgaccg gcttactgct taatttgatg
    35581 cctggcagtt ccctactct
//
LOCUS       DAOFQD010000041        34970 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.041, whole genome shotgun sequence.
ACCESSION   DAOFQD010000041 DAOFQD010000000
VERSION     DAOFQD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.041"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            118..702
                     /locus_tag="QBF46_002032"
     CDS             118..702
                     /locus_tag="QBF46_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6674215.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            807..1547
                     /locus_tag="QBF46_002033"
     CDS             807..1547
                     /locus_tag="QBF46_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDQ6674216.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWSWSGSEGKASLRVANPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKANSAKVHFYAINDFGGAIEGNARL"
     gene            1582..4179
                     /gene="htrE"
                     /locus_tag="QBF46_002034"
     CDS             1582..4179
                     /gene="htrE"
                     /locus_tag="QBF46_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDQ6674217.1"
                     /translation="MTIKYTKNYHHLTRIATFCALLYCNSAFSAELVEYDHTFLMGQN
                     ASNIDLSRYSEGNPAIPGVYDVSVYVNDQPIINQSITFVAIEGKKNAQACITLKNLLQ
                     FHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMKN
                     YQNYVDPSLWENGINAAMLSYNLNGYHSETPGRKNESIYAAFNGGMNLGAWRLRASGN
                     YNWMTDSGSNYDFKNRYVQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRML
                     PPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVTI
                     EESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNNY
                     LTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWNK
                     LFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVTV
                     SINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRTWNE
                     DGDTDDSVYLSFTIPIEKLLGTEQRNSGFQSIDTQISSDFKGNNQLNVSSSGYSDNAR
                     VSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSISANSDNSRQVSLSTDGGFVL
                     HSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIA
                     LDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIY
                     DEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSII
                     LNGIRCQIQ"
     gene            4196..4765
                     /locus_tag="QBF46_002035"
     CDS             4196..4765
                     /locus_tag="QBF46_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6674218.1"
                     /translation="MIKTTPHKIVILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKQTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4777..5382
                     /gene="yadL"
                     /locus_tag="QBF46_002036"
     CDS             4777..5382
                     /gene="yadL"
                     /locus_tag="QBF46_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDQ6674219.1"
                     /translation="MMTFKNLRYGLSSSVVLAASLFSVLSYAATDSIGLTVITTVEMG
                     TCTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKR
                     ATCEGTANDGAGFANGSTAADKASAVAVEVWSTVTPATGSATQFSCVTPASQEVTIST
                     AANAVVYYPMSARLVVEKNKTVNNVTAGKFSAPATFTVTYN"
     gene            5409..6005
                     /locus_tag="QBF46_002037"
                     /pseudo
     CDS             5409..6005
                     /locus_tag="QBF46_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            6055..7311
                     /locus_tag="QBF46_002038"
     CDS             6055..7311
                     /locus_tag="QBF46_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDQ6674220.1"
                     /translation="MKIFFRYFLFLALCSCCYTATANNVSYVVGNNYGVGPSDQKWRE
                     TGPNGDVTVKFRYGSDTNNLVFYKPTQLSPTGVSLKWAQLDSASGGGFLYCNRSGNAS
                     GTAMRIENKMVDSGKSYGGHKLFKTSVPGLYYTLAISNIWSAYTSTDINPSGMYIGDS
                     TEQYFNWRGESEQTLYWSCNNANTTQKYWAVGGVMQTLTIEFYTDTDFNPTTNQRVTL
                     SRTDNYLYSFKGYGVGIGINEHSYFLRVDFDLTDIVLTNPTCFTAALSGPTVSGSTVK
                     MGDYSPGQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKELLANTLTGNNAA
                     KGVGVLIEGLKNTKSAQMVLKPNDSTSIYKDYETENDTTGGIFPDQGNGTSQPLHFQA
                     TLKQDGNIAIEPGEFKATSTFQVTYP"
     gene            7424..8218
                     /gene="panB"
                     /locus_tag="QBF46_002039"
     CDS             7424..8218
                     /gene="panB"
                     /locus_tag="QBF46_002039"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDQ6674221.1"
                     /translation="MKPTTISLLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8230..9081
                     /gene="panC"
                     /locus_tag="QBF46_002040"
     CDS             8230..9081
                     /gene="panC"
                     /locus_tag="QBF46_002040"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDQ6674222.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMSFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9155..10057)
                     /gene="rpnC"
                     /locus_tag="QBF46_002041"
     CDS             complement(9155..10057)
                     /gene="rpnC"
                     /locus_tag="QBF46_002041"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDQ6674223.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEVSQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10331..10711
                     /gene="panD"
                     /locus_tag="QBF46_002042"
     CDS             10331..10711
                     /gene="panD"
                     /locus_tag="QBF46_002042"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDQ6674224.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10715..11944)
                     /locus_tag="QBF46_002043"
     CDS             complement(10715..11944)
                     /locus_tag="QBF46_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDQ6674225.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLSVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12008..12448)
                     /locus_tag="QBF46_002044"
     CDS             complement(12008..12448)
                     /locus_tag="QBF46_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDQ6674226.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12553..13323)
                     /locus_tag="QBF46_002045"
     CDS             complement(12553..13323)
                     /locus_tag="QBF46_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6674227.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13320..14246)
                     /locus_tag="QBF46_002046"
     CDS             complement(13320..14246)
                     /locus_tag="QBF46_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6674228.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14355..15017
                     /gene="can"
                     /locus_tag="QBF46_002047"
     CDS             14355..15017
                     /gene="can"
                     /locus_tag="QBF46_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDQ6674229.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15058..15594)
                     /gene="hpt"
                     /locus_tag="QBF46_002048"
     CDS             complement(15058..15594)
                     /gene="hpt"
                     /locus_tag="QBF46_002048"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDQ6674230.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15800..18190
                     /gene="gcd"
                     /locus_tag="QBF46_002049"
     CDS             15800..18190
                     /gene="gcd"
                     /locus_tag="QBF46_002049"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDQ6674231.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQI
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18392..19942)
                     /gene="cueO"
                     /locus_tag="QBF46_002050"
     CDS             complement(18392..19942)
                     /gene="cueO"
                     /locus_tag="QBF46_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDQ6674232.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMEHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20108..20455
                     /locus_tag="QBF46_002051"
     CDS             20108..20455
                     /locus_tag="QBF46_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDQ6674233.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20561..21427
                     /gene="speE"
                     /locus_tag="QBF46_002052"
     CDS             20561..21427
                     /gene="speE"
                     /locus_tag="QBF46_002052"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDQ6674234.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCHQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21443..22237
                     /gene="speD"
                     /locus_tag="QBF46_002053"
     CDS             21443..22237
                     /gene="speD"
                     /locus_tag="QBF46_002053"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDQ6674235.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22275..22637)
                     /locus_tag="QBF46_002054"
     CDS             complement(22275..22637)
                     /locus_tag="QBF46_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDQ6674236.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22813..25410)
                     /gene="acnB"
                     /locus_tag="QBF46_002055"
     CDS             complement(22813..25410)
                     /gene="acnB"
                     /locus_tag="QBF46_002055"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDQ6674237.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25765..27618
                     /locus_tag="QBF46_002056"
     CDS             25765..27618
                     /locus_tag="QBF46_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDQ6674238.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTFPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAANQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFHDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQSASPEQRQ
                     AVREKMQTNPQNQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(27689..29113)
                     /gene="lpdA"
                     /locus_tag="QBF46_002057"
     CDS             complement(27689..29113)
                     /gene="lpdA"
                     /locus_tag="QBF46_002057"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDQ6674239.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29321..31213)
                     /gene="aceF"
                     /locus_tag="QBF46_002058"
     CDS             complement(29321..31213)
                     /gene="aceF"
                     /locus_tag="QBF46_002058"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDQ6674240.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31228..33891)
                     /gene="aceE"
                     /locus_tag="QBF46_002059"
     CDS             complement(31228..33891)
                     /gene="aceE"
                     /locus_tag="QBF46_002059"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDQ6674241.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34052..34816)
                     /gene="pdhR"
                     /locus_tag="QBF46_002060"
     CDS             complement(34052..34816)
                     /gene="pdhR"
                     /locus_tag="QBF46_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDQ6674242.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8809 a         9080 c         8418 g         8663 t
ORIGIN      
        1 ttgggtaaca aatatcattg ctcattaata tctaacattt gtttttccta tttgtgtcat
       61 gaatagtcat tcttcaaaca tgtagtctga ctaatggaat tttattaaag gatttttatg
      121 tctaaaaaat taggttttgc cctgtctggt cttatgctgg cgatggttgc aggtactgct
      181 tctgctgata tggacggcgg tcagttaaat atcagtggtc tggttgttga taacacctgt
      241 gaaacccgcg ttgacggcgg taacaaggat ggtctgatcc tgctgcaaac cgcaaccgtt
      301 ggtgaaatcg atgctggcgt actgaatgac accgttggcg ctaaagctaa acctttcagc
      361 atcaccgttg actgcagcaa agcaaatccg aatccaggta gcacggctaa aatgaccttc
      421 ggttctgttt tctttggtaa cagcaaaggg actttgaaca acgacatgtc tattaacaac
      481 ccatctgacg gcgttaatat cgctctacac aatattgatg gttccactat caaacaggtt
      541 caaattaaca accctggcga tgtgtatact aaagccctgg atgcaacgac aaaatctgct
      601 gtttatgatt ttaaagcgtc ttacgttcgt gccgtcgcag accaaacagc aactgctggt
      661 tatgtaaaaa ctaacactgc atacaccatt acttatcagt aagtattcac caattaaatt
      721 gattgaatga atatacaggg aatagtatta ataatttata tttttatatt attccctgtt
      781 ttaattaact ctgtcaggga tggtttatgt tttttaacac caaacataca acagctttat
      841 gcttcgtaac ctgtatggct tttagttcat cgtctattgc ggacattgtc atttcgggta
      901 ctcgcgtaat atataaaagc gatcaaaaaa gtgtcaacgt acgtctggaa aataaaggga
      961 ataacccgtt gcttgtccag agttggttag atactggcga tgacaacgct gagccgggca
     1021 gtattacagt cccttttact gccacgccgc cagtatcgcg tattgatgcc aaacgtgggc
     1081 aaacaatcaa attaatgtac accgccagca catcactgcc taaagacaga gagagtgtgt
     1141 tctggtttaa cgtactggaa gttccaccaa aaccagatgc agaaaaggtc gcgaatcaaa
     1201 gcctgctgca actggcattt cgtacacgta taaaactttt ctatcgcccg gatggattga
     1261 agggaaatcc ctctgaagcc ccgttagccc tgaagtggtc ctggtcaggt tcagaaggta
     1321 aggcgtcatt acgagtggct aacccaaccc cttactacgt ctcttttagc agtggtgatt
     1381 tggaagccag cggtaaacgc tatccgattg atgtgaaaat gattgcacca ttcagtgatg
     1441 aagtcatgaa agtcactggt cttaatggca aagcgaattc tgcaaaagtg catttttacg
     1501 ccattaatga ctttggtggc gcaattgaag gtaatgccag gctgtaatca ggcaaggata
     1561 taattccgca ggaagcatag cgtgactata aaatatacta aaaattatca tcatctgacc
     1621 cgtatcgcca cgttttgcgc gctgctgtat tgcaacagtg ctttcagtgc tgaactcgtt
     1681 gaatatgacc ataccttcct gatggggcag aatgcatcta atattgatct cagccggtac
     1741 agtgaaggta accccgctat accgggtgtt tacgacgtca gtgtttatgt aaacgaccaa
     1801 ccaatcatta accaaagtat tacatttgtc gcaattgaag gaaaaaagaa cgcccaggct
     1861 tgtatcacat taaagaattt attgcagttt catattaatt ctcccgatat aaataacgaa
     1921 aaagccgttc tgcttgccag ggatgaaacg ctcggcaatt gcctcaattt gacggaaatt
     1981 atccctcagg cttctgttcg ttatgacgtt aacgatcaac gtctggatat agacgttcct
     2041 caagcttggg taatgaaaaa ttaccaaaac tatgttgatc catcgttatg ggaaaacggc
     2101 attaatgcgg ccatgttgtc atacaacctc aacggatatc atagtgaaac ccctggtcga
     2161 aaaaatgaaa gcatttatgc tgcatttaac ggtgggatga atttaggtgc atggcgactg
     2221 cgtgcctcgg gcaactacaa ctggatgacc gattctggca gtaattatga ttttaagaat
     2281 cggtatgttc agcgtgatat cgcctcgctg cgttctcaac tcattcttgg tgagtcttat
     2341 acgacgggcg aaacctttga ttccgtcagt atccgaggca ttcgtttata cagtgacagc
     2401 cgcatgttgc ctccgacttt agccagcttt gcgcctatca ttcatggcgt tgccaatacc
     2461 aacgccaaag tcactattac gcaaggtggc tataagattt atgaaacgac ggtgccgcca
     2521 ggcgctttcg tcattgatga tctgagtccg tcagggtacg gcagcgatct tattgttacc
     2581 atcgaagaat ccgatggctc aaagcggaca ttctcgcaac ctttctcatc cgttgttcaa
     2641 atgttacgcc ctggcgttgg acgttgggat attagcggcg gtcaggtctt aaaagatgat
     2701 attcaggatg agcctaattt atttcaggcc agctactact acggcctgaa taactatctg
     2761 acgggttata ccggtattca gataaccgat aataactata ccgctggttt gttaggtctt
     2821 ggtctgaata cttcagttgg tgcattttct ttcgatgtga ctcattccaa tgttcgtatc
     2881 ccggatgata aaacatacca ggggcaaagt tatcgtgttt cctggaacaa gttattcgaa
     2941 gaaacaagta cttcactgaa tatcgcggcc tatcgctatt cgacacagaa ttaccttggt
     3001 cttaatgatg cactaactct aattgatgaa gtgaaacatc ccgaacaaga tcttgaaccg
     3061 aaatccatgc gtaattactc acgcatgaaa aatcaggtta cggtcagtat taaccaaccg
     3121 ttaaaatttg agaaaaaaga ttacggttca ttttatcttt ccggaagttg gtccgattac
     3181 tgggcttccg gacaaaatcg tagcaattac tctattggct acagtaacag tgcatcctgg
     3241 ggcagctaca gtgtcagtgc ccagcgtaca tggaatgaag acggcgacac tgacgatagc
     3301 gtttatctca gtttcaccat tccaattgaa aaattacttg gcactgaaca acggaattca
     3361 ggtttccaga gtattgatac tcaaataagc agtgacttta agggtaataa ccaactcaac
     3421 gtcagtagca gtggctatag cgataacgct cgcgtcagtt atagcgtgaa tactggctat
     3481 acgatgaata aagccagcaa agatttgagt tatgttgggg gttatgccag ctatgagtca
     3541 ccatggggaa cgctggcagg ttcaatttct gcaaatagcg ataacagccg tcaagtttct
     3601 ctcagcaccg acggtggttt tgtattgcat agcggtggac tgactttcag taatgatagt
     3661 tttagcgact ccgatacact ggcggtagtt caggctccag gtgctcaagg agcgcgaata
     3721 aattatggca acagtactat cgatcgatgg ggttatggtg tcaccagcgc tctttctcct
     3781 tatcatgaaa accgtatcgc gctggatatc aacgatcttg agaacgatgt tgaattaaaa
     3841 agtaccagtg cagtagctgt accgcgtcag ggttcagtcg tctttgctga ttttgaaacc
     3901 gtgcaagggc aatcagccat tatgaacatc acacgaagtg atggtaaaaa tattccattt
     3961 gctgcagata tttatgatga gcaaggcaat gtcattggta atgttggaca gggtggacaa
     4021 gcatttgttc gtggtattga gcagcaggga aatatcagca ttaaatggct cgaagaaagt
     4081 aaaccagtaa gttgtcttgc gcattatcaa caaagctcag aagcagaaaa aatagcacaa
     4141 tctattattc tgaatggaat tcggtgtcag attcagtaac tacaagggac gtcaaatgat
     4201 aaaaacaacg ccacataaaa tagtgatact gatgggaata ttattatcac cctcagtatt
     4261 tgcaacggat attaatgtag agtttacagc cactgtcaaa gcgacaacct gtaacatcac
     4321 acttactggt aataacgtca cgaatgatgg caataataac tacacattga gaatccctaa
     4381 gatgggtctg gataagatcg cgaataaaac gacagaatct caggctgatt ttaaactggt
     4441 tgccagtggg tgcagcagtg gcatcagttg gattgatacc actctgaccg gaaatgcatc
     4501 atcaagctca cctaagctta ttataccgca gtctggtgat tcatcttcga cgacaagtaa
     4561 tatcggtatg ggtttcaaaa aacagactac tgatgatgcc actttcctta aacctaacag
     4621 tgcggaaaag atacgctgga gcacagacga gatgcagccc gataagggtc ttgaaatgac
     4681 cgttgcgcta cgtgaaacag atgcagggca aggcgtaccg gggaattttc gtgcactggc
     4741 gacgtttaat ttcatctatc aataagaagg ataattatga tgacatttaa aaatttacgt
     4801 tatggattat ccagcagtgt cgttttggct gcctcattgt tcagcgtact ctcttatgcg
     4861 gcaacagaca gtattggact gaccgttatt actactgtag aaatgggtac ttgtaccgct
     4921 acattagtaa atgactctga tcaggacatt tctgttgttg attttggtga tgtatatatt
     4981 tctgaaatca atgccaagac caaagtaaaa acattcaaac tcaaattcaa agactgtgcg
     5041 ggtatcccca ataaaaaagc gcaaataaaa ttaaccaagc gagccacatg cgaggggact
     5101 gctaatgacg gtgcagggtt cgcaaatggt tctacagccg cagataaagc aagtgccgtc
     5161 gccgttgaag tctggagcac tgtaactccg gcaacaggga gcgcaacaca atttagttgt
     5221 gtgaccccag catctcaaga ggtcactatc tccactgctg ccaacgcggt cgtttattac
     5281 ccaatgagtg ctcgcctggt cgtagaaaaa aataaaaccg taaacaatgt tacggcgggt
     5341 aaattttctg cacctgccac atttacagta acctataact aacagactta cagattcagg
     5401 aactatatat gcatcccact caacgtaagc tgatgaagag aataattctg tttctgtcat
     5461 tactgtttcc cattacctgt ccagcgatcg ctggacagga tattgatctt gttgccaatg
     5521 taaaaaacag cacttgcaaa agcggaatca gtaaccaggg taatattgat cttggcgtcg
     5581 ttggggtggg atatttttca ggtaatgtta ctcctgaaag ttatcaacct ggtggaaaag
     5641 agttcactgt cactgtatcc gactgtgcat tacagggaac tggcgatgtg ttaaatcagt
     5701 tacatattga ttttcgcgca ctcagtggcg taatggcgac aggttccagg caaatatttg
     5761 ccaatgaaga caacgctggg gctaaaaatg ttggggtagt tatattctcc atttaggacc
     5821 cggcaaatac cttcaacgtt ctcggtgcca ctggtaactc acgttccgtt tatccggtta
     5881 tgtcatcagc actaaacaat tcatcctgga aatttagcac ccgaatgcaa aaaatcgatc
     5941 cagcgctgaa tgttacctct ggcccactta taagccatgt gttagtggat atttactacg
     6001 aataacagac aaagtgctgc aagcattatt ttgcagcata aaaatggagt aaaaatgaag
     6061 atattcttca gatactttct ttttttagca ctgtgctctt gttgttatac cgcaacagct
     6121 aataacgtca gctatgttgt tggcaataat tacggtgttg gcccatcaga ccaaaaatgg
     6181 cgagaaacag gccccaatgg agatgtcacc gtcaaattcc ggtatggttc agacaccaat
     6241 aatttagttt tttataaacc aacccaactt agcccaaccg gggtgtcatt aaaatgggcg
     6301 cagttagatt ccgcttcagg tggcggcttt ctttactgta acagaagtgg taacgcaagc
     6361 ggcacggcaa tgcgtattga aaataaaatg gttgattccg gcaaaagtta tggtgggcat
     6421 aaactcttta agacatctgt cccaggcctg tattacacat tagcaatatc aaacatttgg
     6481 tcagcatata catccactga tatcaaccca tcaggcatgt atattggcga ttctacagaa
     6541 caatatttta actggcgtgg cgaaagcgag caaacactat attggtcttg caacaacgcg
     6601 aacaccaccc aaaaatattg ggccgttggt ggcgttatgc aaacgctcac gattgaattt
     6661 tataccgata ccgactttaa cccgacaacg aaccaacggg taacattatc cagaacagat
     6721 aattatctct actcattcaa aggatatggt gttggcattg gtatcaacga acacagctat
     6781 tttcttaggg ttgactttga cctgaccgat atcgtattaa caaaccctac ctgttttact
     6841 gcggcgctta gtggcccaac ggtaagtggt tcaacggtta aaatgggtga ttatagccct
     6901 gggcaaataa aaaatggcgc aacagcggtt ccttttgata tcacgctaca gaactgtatt
     6961 cgtgttcgca atatagaaac aaaactcatg tcaaacaagg tgggtagtgt aagcaaagaa
     7021 cttctggcta acacactcac aggaaataat gcagcaaaag gcgttggtgt actgattgaa
     7081 ggtttaaaaa acactaaaag tgcacaaatg gtactaaaac caaatgattc aacgtctatt
     7141 tataaagatt atgaaacaga aaatgacact accggaggga ttttcccgga tcaaggcaac
     7201 gggacatcac aacctcttca cttccaggca acattaaagc aagacgggaa tattgctatc
     7261 gaacccggcg aatttaaagc caccagtact ttccaggtaa cctatccctg ataacattgt
     7321 ggcaccgtct ggtaatggcg gtgtcacaac agagaaatca ttgcccccac caatgtgact
     7381 gataaaatgc cagacagcac aacatcattt atcaggatac gttatgaaac caaccaccat
     7441 ctccttactg cagaagtaca aacaggaaaa aaaacgtttc gcgaccatca ccgcttatga
     7501 ctatagcttc gccaaactct ttgctgatga aggacttaac gtcatgctgg taggcgattc
     7561 gctgggcatg acggttcagg gacacgactc caccctgcca gttaccgttg ccgatatcgc
     7621 ctaccacact gccgccgtac gtcgcggcgc accaaactgc ctgctgttgg ctgacctgcc
     7681 gtttatggcg tatgccacac cggaacaagc ctttgaaaac gccgcaacgg ttatgcgtgc
     7741 cggtgccaac atggtcaaaa ttgaaggcgg tgagtggctg gtagaaaccg tacaaatgct
     7801 gaccgaacgt gccgttcctg tatgtggtca cttaggttta accccacagt cagtgaatat
     7861 tttcggtggc tacaaagtac aggggcgcgg cgatgaagcg ggtgatcaac tgctcagcga
     7921 tgcattagcc ttagaagccg ctggggcaca gttgctggtg ctggaatgcg tgccggttga
     7981 actggcaaaa cgtattaccg aagcactggc gatcccggtt attggcattg gcgcaggcaa
     8041 cgtcactgac gggcagatcc tcgtgatgca cgacgccttt ggtattaccg gcggccacat
     8101 tcctaaattc gctaaaaatt tcctcgccga aacgggcgac atccgcgcgg ctgtgcggca
     8161 gtatatggct gaagtggagt ccggcgttta tccgggcgaa gaacacagtt tccattaagg
     8221 agtcacgttg tgttaattat cgaaaccctg ccgctgctgc gtcagcaaat tcgccgcctg
     8281 cgtatggaag gcaagcgcgt ggcgctggtg cctaccatgg gtaacctgca cgatggccat
     8341 atgaagctgg tcgacgaagc caaagcccgc gccgatgtgg tcgtcgtcag tattttcgtt
     8401 aacccgatgc agttcgaccg cccggaagat ctggctcgtt atccacggac cttgcaggaa
     8461 gactgcgaaa agttaaacaa acgtaaagtc gatttggttt tcgcaccctc ggtaaaagag
     8521 atctacccga acggtacaga aacccacacc tatgttgatg ttcctggtct ttcgaccatg
     8581 ctggaaggtg ccagccgtcc gggacatttt cgcggcgttt cgactattgt cagcaagctg
     8641 ttcaacctgg tccagccgga catcgcctgc ttcggtgaaa aagattttca gcaactggcg
     8701 ctgatccgca aaatggttgc tgatatgagc tttgatattg agattgtcgg tgtgccaatt
     8761 atgcgcgcca aagatggtct ggcgctgagt tcccgtaacg gttatctgac ggcagaacaa
     8821 cgcaaaattg cgcccggtct gtacaaagtt ttaagttcga ttgccgacaa attgcaggct
     8881 ggcgaacggg atctcgatga aattattgct attgccgggc aagaactgaa tgaaaaaggc
     8941 ttccgcgccg atgatattca gattcgtgat gccgacacgt tgctggaagt ctctgaaaac
     9001 agcaaacggg cagtgattct ggttgccgcc tggcttggcg atgctcgcct gatcgacaac
     9061 aaaatggtcg agctggcgta atacttaact ggcgctacgg ctgatggcgc cagttattaa
     9121 tttaccccac gtcatctgtc ataacacagg taacttaaat aaggttaatt accttatcaa
     9181 tctcagcaag aggtaaattt gccatctctg caacgtcttc ccgagacatt cctttactta
     9241 gaagacgcag ggcgaattcc tgacttactt cctgtcttcc ctgctgaatc cccttctcaa
     9301 tccctttctc ttcaaaccac tgcgccagcg tcatcataga cttccctccc gtttccctgt
     9361 ctctcagcac accgtaaaac aaatccgctt gttcagtatg accgcgttgc agcatatagt
     9421 tttgcatggc aactaactga cttccgctag tgtacccttc gtcgatcagc gtgaccagtt
     9481 gctcaagcaa taacattaag tcgcgctggc gaatatgttt ttgcagtagt tcgagaatcg
     9541 caatccgccg atgttgcatg atttcgtcat ccggtgtgat ggtgatatcc accagcggga
     9601 aaggactgtt atagacgcgt cgcgccagct ccggcgagta aaacatatca aaccagcaca
     9661 ttgatagcgg ataaggtgtg gcctcgccct gataaaacag tatcggcacc accagcggca
     9721 gcttatcgtg gtcagcctcc agatgccggt gcatggcggc tatagaataa cgcatcatgc
     9781 gaaaggccat tttcttatcc ggcttgcttt ggtgttcaat cacaacatgc agataaccgg
     9841 gattgccctg catttgcacg gaatagagca cgtccgtgct gtgtcctttc aggctctctt
     9901 caatgaaact ccccgactct aaatgaagcg tgttgaggtc acaaagttcg cgtaattcca
     9961 ctggcaaatg tatctccaga aagtcgcgag ccgtctccgc atgcattaaa aattgtttaa
    10021 ataccgcgtc atgcggtgtg gtactcggtg catccatctt ccctgtcttc cgtgtggctt
    10081 tgcgtgaagg cagtatccag tcaaggcgcg ctatcgtcag ccacctcttt attgtttacc
    10141 gagcagcgtt cagagaaata tcaattcgtt acaggcgata catggcacgc ttcggcgcgg
    10201 tacgcaaact gctgacgcaa atgccgtcag atgagtagac actaaacaaa aatcgggcaa
    10261 tactgcgtga gaatttcctc aaagcaggca tcgcctgctt cgttaacgac agggtagaaa
    10321 ggtagaagtt atgattcgca cgatgctgca gggcaaactc caccgcgtga aagtgactca
    10381 tgcggacctg cactatgaag gttcttgcgc cattgaccag gattttcttg acgcagccgg
    10441 tattctcgaa aacgaagcca tcgatatctg gaatgtcacc aacggcaagc gtttctccac
    10501 ttatgccatc gcggcagaac gcggttcgag aattatttct gttaacggtg cggcggccca
    10561 ctgcgccagt gtcggcgata ttgtcatcat cgccagcttc gttaccatgc cagatgaaga
    10621 agctcgcacc tggcgaccca acgtcgccta ttttgaaggc gacaatgaaa tgaaacgtac
    10681 cgcgaaagcg attccggtac aggttgcttg attgttatcc ctgcggctgg ttactcacca
    10741 gccgcgacat cgtctccagc gaatccgttc ttaagatata aagtcgtttt agtaacaacg
    10801 gattatcccc cggttttact ttgcctttca tggttgtcac cgccaggtga aatccggcat
    10861 cgtttgctgc cttcacggct ttgtcattaa atccgccaaa cggatacgaa agataccaga
    10921 catgcggatt aaattgcgcc agagcgcggc gtgaacgtgc aaaatcaaac agaatattgt
    10981 gctcactacg gctcagtaat atgggtcggc gataaccatc tacccgatgc aaaaaatggg
    11041 tatgtgactg gaaatcaaat acatcgcgaa tttcgttaag ctcagaaacg ctcataaatt
    11101 gcagcgattt tgggttccac ttctgcgggt gacgtttgat gcgtgaggta acaataaacg
    11161 ccgtcgcctt catgccatat tgtttcaaca caggatacgc atagcggctc accgacttga
    11221 ggccatcatc aaaggtaatc accaccgctc gcgcagggag attgatctta ttcttcacgt
    11281 agccttccag ctgcaccatg ctcagtgtcg cgtatcccct gtcacgcagc caggccatct
    11341 ggttattgaa agcgcgtacc gaggtggtcg tcgaagtatg gcgaaaacgg gtgttttctt
    11401 cgtcgcgcag aatatggtga taggttagca ccgacaggcc attatcgggt tgggcatcca
    11461 gtgcgctgat ataggccagt cgatcgccaa tacggatctg ataccaggtt tgatttaacc
    11521 tgtctttcag tttatgcaaa atcgggtagc gcaaattgtc cgccagtacc ccaaatggcg
    11581 cacttcccgc actcggcgcg ttatagaccg gcgtatcttt ccaggtaact aagttctgat
    11641 tactcagcgg cttgttgagg tcgcccaaac cgtcttcaac tttttgtcgc ccctgaaccg
    11701 gctcgagatg acctttatcg ataaaacctt tgccaaagcc aaaattaaat acgtaataac
    11761 ttgcggcagt gggctccacg gcaatgattt gtccggcccg aatattcccc acggtcacca
    11821 tcttgtcacc aatttgcgcc cagaccgcag cattttcgat ggtttgcata taacgggcag
    11881 gtaacgcggc actgacactt gcagtaaaca gcatcagcag gagaataaca gcttgtttat
    11941 acatgaaaaa ataaccgagg gtgaaacaac gcttttattg tagcaaaagc gccatcagaa
    12001 tcgcgggtta atactcatac aaattatgtt tgaggacgaa aggcggattt ttttgttgtt
    12061 ggtgccaaag actactcacc tccggcccac ccagttcgac aatacgctcg cgaaactctg
    12121 aactggtcat ggtttcacgg caagccatca tctgttcaat taacggtaac gtgacaccag
    12181 aaatcacttc gcaacgggag tgtttgtggc ttaataatga agccacgcga tacggtggcg
    12241 ctccggctat atcggttaag aagatgacac cctcaccaga gtccgcttca tgcagagcat
    12301 cgcacatcat gcggctgagc atattagaac ttaatccgcg ccagaaattc acggcccggc
    12361 actgaagaag cgccccatgt tttttctcca gcgcatccag tatctcttgc gcccggtcat
    12421 cgtgacaggt aattacccaa cctaacatcg ccttcctcct tagccgaaaa tcagtctacc
    12481 ctgaccattg ttgataaagg gtgatagccg tcaaaactcc tctcccctaa atccagagga
    12541 gaggaaatag ccttagctac gcaaaccacg tccacgttgg atcagcgacc aacagatcaa
    12601 ataaaacgcc acaataaaga ccaccagtac gccaaaggta gtgaccagcg gaacatcatt
    12661 gataccgagg aagccgtagc ggaaaccact gatcatataa acgattgggt tcaggtgcga
    12721 cagcccttgc cagaacggcg gcaacaaagt cagtgagtaa aagaccccgc ccaaatacgt
    12781 gagtggcgtt aacacaaagg ttggcaccag gctgatgtca tcgaacgttt tggcaaacac
    12841 gccgttcagc aagcccgcaa gggagaacaa caccgccgta agcaccagcg ttaaagcgac
    12901 aaatacccac gaatgcacct gaaacggcac aaaaaacagt gaaattgccg tcaccagaat
    12961 gccaacaaac agaccacgcg ccacgccacc gccgacatat ccggcaataa tgacgtgagt
    13021 cggaaccggc gctaccagca gctcttcaat attacgctgg aacttggcac caaaaaatga
    13081 tgacgcgacg ttagcgtagg cgttggtgat caccgacatc atgatcagcc ccggtacgat
    13141 gaactgcatg tagctgaagc catgcatatc gccaatacgc gaaccaatca ggttaccgaa
    13201 gataataaag taaagggtca tggtgatgac tggcggcacc agcgtctgca cccagatacg
    13261 cataaagcga tggatctctt tcgcccagat gctttttagc gccacccagt aaagatgcat
    13321 catgcgcgat ctccttgttt ttcattaacc agtgaaacaa acagctcttc cagacggtta
    13381 gctttgttac gcatacttaa tacctgaatg ccctgctcac ttaactgcgt aaatacgctg
    13441 ttgatcccct gctcacgcag cacttcaact tccagcgtcg cggtatcgac cagtcgatac
    13501 tgatagccat cgagcttcgg taacggactt ttcggtgcga gatcgagaat aaaggtttcc
    13561 gatttcagct tcgccagcag cgccttcatc gaggtatttt ccaccagctc accatgttga
    13621 ataatgccga tattgcggca cagcatttct gcttcttcca ggtagtgtgt ggtgagaatg
    13681 atggtggtgc ctttgtcgtt taaatccttc aaaaagcccc acattgagcg gcgaagttca
    13741 atatccacgc ctgcggtcgg ttcgtcgaga atcagtagtt taggctcatg cattaacgca
    13801 cgggcaatca ttaaacgacg cttcatcccg ccagataaca tacgcgcacg ttcgttgcgt
    13861 tttccccata aatcgagctg cttaagatac ttttcgctgc ggatgtacgc ttctttgcgc
    13921 tccacaccgt agtaccctgc ctgattcacc acaatttgct gcacggtttc aaacgggttg
    13981 aaattaaatt cctgcggcac cagtcccaac tgacgtttag cgttaacgac atctttctcg
    14041 agatcgtaac caaatacgct gacccgcccg gaggttttat ttaccagaga gctgataata
    14101 ccgatagtgg tcgatttccc ggccccgttt ggcccgagaa gcgcataaaa atcacccgct
    14161 tcgacctgca aatctattcc acgaagcgcc tgaacgccgc ctggataggt ttttttaagt
    14221 tgttgaagtt ccagtgcaat ggtcataaat ttttacttac cttacgttct tacactttat
    14281 ctgtggttta aatcgtcccg gagttgccct atattagcca aacgtaatta tttggttaca
    14341 ggtcgttaac ctccatgaaa gacatagata cactcatcag caacaatgca ctatggtcaa
    14401 aaatgctggt ggaagaggat cccgggtttt ttgagaaact ggcacaagcg caaaaaccgc
    14461 gctttctatg gattggatgt tccgacagtc gcgttcctgc agaacgttta accggtcttg
    14521 agccgggcga actctttgtt caccgtaatg ttgctaacct ggtcattcac accgacctga
    14581 actgcctttc cgtggttcag tatgcagtgg atgtactcga agttgaacac attattatct
    14641 gtggccacta cggttgcggc ggcgtacaag ccgcagttga aaacccggaa ctggggctta
    14701 tcaacaactg gctgctgcat atccgcgata tctggttcaa acatagctca ttgctcggcg
    14761 aaatgccgca agagcgccgt ctggatacct tgtgtgaact gaacgtcatg gaacaggttt
    14821 ataacctggg ccactccacc attatgcaat cagcgtggaa acgcgggcag aaagttacca
    14881 ttcacggctg ggcctacggc attcacgacg gcttgctgcg tgatctggat gtcacagcga
    14941 ccaaccgcga aacccttgag caacgttacc gtcacgggat ttccaacctc aagctgaaac
    15001 acgccaacca caaataaaaa tgccatgccg gatgcaacac atccggcaac ttcacactta
    15061 ctcgtccagc agaatcactt tgccgatata cggcagatga cggtaacgct gtgcgtaatc
    15121 aatgccgtaa cccaccacaa actcatccgg gatcgagaaa ccgataaatt caaccgggac
    15181 gttcacttca cgacgggacg gtttatccag cagcgtacaa atcgccaacg acttcggttc
    15241 gcgcaggctt aagatctcac gcactttcga cagtgtattc cccgagtcga tgatatcttc
    15301 aacaatcagc acgtccttgc cacggatatc ttcatccaga tctttgagga ttttcacatc
    15361 acgggtggtg gacatgccgc taccgtagct ggaggcggtc ataaagtcga cttcatgaga
    15421 tacctgaact tcacggcaca ggtccgccat aaacataaat gagccacgca gcagacccac
    15481 cagcaccata tcgctgccgc tgtctttgta acgctcagta atctgacgac ccagttcggc
    15541 gatacgcgct ttaatctccg cttcggggat cattacttct acagtatgtt tcatatctct
    15601 aaccatatga tttaaaaaca aatcacttcg cacgggcagg ttttggcacc gcaacaaagc
    15661 gcaagacacg cagtatacca gtaaaactat tcattcgcgg aatctgttaa taaagctcga
    15721 attgtgatga cgatcacaca tgttaaaacc tataattaat tgctactaaa atattaatga
    15781 attgaaatgg tgtctcttta tggcaattaa caatacaggc tcgcgacgat tactcgtcac
    15841 gctaacagcc ctttttgcag cgctttgcgg gctgtatcta ctcattggcg gaggctggct
    15901 ggtcgcgatt ggcggctcct ggtactaccc tatcgctggc cttgtgatgc tcggcgtcgc
    15961 ctggatgctg tggcgcagta aacgtgccgc gctttggctg tacgcagccc tgctgctcgg
    16021 caccatgatt tggggcgtct gggaagttgg tttcgacttc tgggcgctga ctccgcgcag
    16081 cgatattctg gtcttcttcg gcatctggct gatcctgccg tttgtctggc gtcgcctggt
    16141 cattcctgcc agcggcgcag ttgccgcact ggtggtggca ctgctgatta gcggtagtat
    16201 cctgacctgg gccggattta acgatccgca ggaaatcaac ggcaccttaa gcgccgatgc
    16261 cacacctgcg gaagctatct cacccgttgc cgatcaggac tggcctgcct atggtcgcaa
    16321 tcaggaaggt caacgctttt cacctctgaa acaaattaac gccgataacg tccataatct
    16381 gaaagaagcc tgggtgttcc gtactggcga tgtgaagcag ccgaacgatc cgggcgaaat
    16441 caccaatgaa gtgacgccga ttaaagtggg cgacaccctt tacctgtgta ccgctcacca
    16501 gcgcctgttt gcgctcgatg ccgccagcgg caaagagaaa tggcattacg atcctgagct
    16561 gaaaactaac gagtctttcc agcacgtaac ctgccgtggc gtctcttatc atgaagccaa
    16621 agcagaaacg gcttcgccgg aagtaatggc ggattgcccg cgtcgtatca ttcttccggt
    16681 caatgatggt cgactgattg cgattaacgc tgaaaacggc aaactgtgcg aaaccttcgc
    16741 caataaaggc gtgctcaatc tgcaaagcaa tatgccagac accaaaccgg gtctgtatga
    16801 accgacttcg ccaccgatta tcaccgataa aaccatcgtg atggcaggtt cagttaccga
    16861 taacttctca acccgcgaaa cgtctggcgt gatccgtggt tttgatgtca acaccgggga
    16921 gctgctgtgg gcttttgatc ccggcgcgaa agatccgaac gcaatcccgt ctgacgaaca
    16981 cacctttacc tttaactcgc caaactcgtg ggcaccagcg gcctatgacg cgaagctgga
    17041 tctggtgtat ctgccgatgg gcgtgaccac gccggatatc tggggcggta accgcacacc
    17101 ggaacaggaa cgttatgcca gctcgattct ggcgctgaat gccactaccg ggaaactggc
    17161 gtggagctac cagaccgttc accacgacct gtgggatatg gatcttccgg cacagccgac
    17221 gctggcggac atcaccgtta atggtcagaa agtgccagtt atttacgctc cggcaaaaac
    17281 cggcaacatt tttgtgctcg atcgtcgtaa tggcgaactg gtggttccgg caccggaaaa
    17341 accggttccc caaggtgctg ccaaaggcga ttacgttacc cctactcaac cattctcgga
    17401 actgagcttc cgtccgacga aagatttgag cggtgcggat atgtggggag ccaccatgtt
    17461 tgaccaactg gtgtgccgcg tgatgttcca ccagatacgc tatgaaggca ttttcacgcc
    17521 gccatctgaa cagggcacgc tggtcttccc gggtaacctg ggaatgtttg aatggggcgg
    17581 gatttccgtt gatccaaatc gtgaagtggc aattgccaac ccaatggcac tgccgtttgt
    17641 ttcgaaactg atcccacgcg gtccgggtaa cccgatggag cagccgaaag atgccaaagg
    17701 cacgggtacg gaatccggca ttcagccaca gtacggtgta ccgtatggtg tcacgctcaa
    17761 cccgttcctc tcaccatttg gtctgccatg taaacagcca gcatggggtt atatctcggc
    17821 gctggatctg aaaactaatg aagtggtgtg gaagaaacgt attggtacgc cgcaggacag
    17881 tatgccgttc ccgatgcctg taccggtgcc gttcaatatg ggtatgccga tgctgggcgg
    17941 gccaatctcc acggcgggta acgtgctgtt tatcgccgct acggcagata actacctgcg
    18001 cgcttacaac atgagcaacg gtgaaaaact gtggcaaggt cgtttaccag cgggtggtca
    18061 ggcgacgcca atgacctatg aagtgaatgg taagcagtat gtggtgatct ccgcaggcgg
    18121 tcacggttca tttggtacga agatgggcga ctatattgtg gcttatgcgc tgccggatga
    18181 tgtgaagtaa gacttgctca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
    18241 gtgaacgcct tatccagcct acaaaattgt gcaaaatcaa tggattgcac agctagcgta
    18301 ggcctgataa gcgtagcgca tcaggcagat ttgcgtttgt catcagttta atgcccggag
    18361 agatccgggc atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg
    18421 tatcttcatg ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcatcgt
    18481 gattaaactt caccagcact tcgctgacat taccttctac cttaacggta tctttccagc
    18541 ccgcgcgatg agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt
    18601 ggatatggaa cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtatt
    18661 gccctttcgc cgccgcaaac atcggcttgt tcatatcaaa cgcctgaccg ttgattttgt
    18721 tggcatggtg gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgctcca
    18781 tatggcccat catctggctg tgatccatcc cggccatcgc ctgatcgcca tatttctcca
    18841 ttagcatctg catccccatc atatcgagca tcgggtccat agagagttgc agcttgcgta
    18901 ccgtcagccc ttccagcgaa ggtaacgcag gcaggctact taatgtgtct ggcaaagcac
    18961 cggaggcact aatagcaatc ggctgaatcc gcattaccgg atgaggctta tcaaacggcg
    19021 caatcgccat ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttatcgt
    19081 taacctccac cagcacttca aaacgctcgc ccatcagcac cggcagttcg ctcaccttca
    19141 ctggttcagg tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg
    19201 tggcgaaatt gagcgaacgg gcattacagc cattgagcaa acgcaggcgc agccaaccac
    19261 gcggggcagc gtgttgcggg tagattgcac cgttggtcag caacgtatcg ccaaaccagc
    19321 ccacggcggc ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct
    19381 tatcctgaac gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca
    19441 ggatctcgtc atcttcaatc accaccagcc cagccagccc catcgccacc tgtcgcccgg
    19501 ttttgccgtg ctgatgcgga tggaaccagc aggtagcggc aggttgatca acgttcaacg
    19561 tcaccgagcg cttgccacct ggcggaataa ttccctgcgg gccgccgtcg acttcacccg
    19621 gtacttccag cccgtgccag tgcaacgttg tctcttccgt cagttggttg tagatatcaa
    19681 ccgttaccgc tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc
    19741 cccaggtagt tgcagttttc ccgccaaagg tggactggcc tgcgccaata gttaactgaa
    19801 tgcgattacg ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttctgccg
    19861 caaatactgc gcggctccac agcggcaaag ccgaagccac acccagcgcg acggaatatt
    19921 ttaagaaatc acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca
    19981 aacgttgagc atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga
    20041 caataatggt cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt
    20101 agaagcaatg aagacgtttt tcagaacagt gttattcggc agcctgatgg ccgtctgcgc
    20161 aaacagttac gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt
    20221 tgtctttctg aagaacgatt gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc
    20281 actggtcttt ttcgctcagc aaaaccagtg ggacctcagt aattacgaca ccttcgacat
    20341 gaaagccctc ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa
    20401 aaaatgcaaa gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct
    20461 gatacctttt tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc
    20521 gccctttttt tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg
    20581 gcatgaaacg ctacacgacc agtttgggca gtactttgcg gtcgataacg ttctgtatca
    20641 tgaaaagacc gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat
    20701 ggcgctggat ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat
    20761 gacccatgtt ccgctactgg cccatggtca cgcgaaacat gtgctgatta tcggcggcgg
    20821 cgacggtgcc atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt
    20881 ggaaatcgat gcgggtgtcg tatcgttctg ccatcagtat ctacccaacc ataacgccgg
    20941 tagctacgac gatccgcgct ttaagctggt gatcgacgat ggcgtcaatt tcgttaatca
    21001 aaccagccag acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga
    21061 aagccttttc acttcggcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat
    21121 cttcgtcgca caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg
    21181 caaactcagc cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta
    21241 cggcggtatc atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac
    21301 cgaaattatt caggcgcgtt ttctcgcctc tggcctgaaa tgccgttatt acaatccggc
    21361 aatccatacg gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc
    21421 gtcctaagga gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac
    21481 caaaagtctg agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga
    21541 cggttatatt gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc
    21601 agaaacctgt tccattatcg gggctaatat tcttaacatc gcccgccagg attacgaacc
    21661 acagggtgcc agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga
    21721 caaaacagaa caccccggcc cactgccaga aacggtcgtt gcccatcttg ataaaagtca
    21781 tatttgcgta catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc
    21841 cgatattgaa gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat
    21901 ccaccagctt gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttacccgcga
    21961 cattaacggt atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc
    22021 tgacgatatg aaggcgctgt atgacatggt ggatgtgaac gtctatcagg aaaatatctt
    22081 ccataccaag atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc
    22141 ggaagactta accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg
    22201 cgagatttat tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc
    22261 tccgccagat ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga
    22321 aaatcctcaa cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc
    22381 atttcatcgc cagcgaattc cagttgattg gcgcgaacca tcacctcttc accgtccatc
    22441 cacagggtgt attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc
    22501 gctgcttgtt ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag
    22561 tgcccgacca cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc
    22621 agaaattcgt aatccatagt gtgttcctct gctcccggca atgcaccgaa atcgcattgc
    22681 gccattgatg taattatcgc agccttaccg gagaaaaaca gcggaacggc gatggtttag
    22741 aaataaaaaa atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg
    22801 tgcgctgact ttttaaaccg cagtctggaa aatcacccca tcggctttct cggtgtactg
    22861 agaaagctgg ttgaagttca gataacggta agtatcaacg gctgttttat ctacctgcgc
    22921 cacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg caacagcagc
    22981 cagttccgca gaagccagga agacattcgc gccagtaccc agacggttcg ggaagttacg
    23041 ggtagaggtg gaaaccaccg ttgcaccgtc cgccacacgc gcctggttac ccatacacag
    23101 ggaacagcca gggatctcga tacgcgcacc actcttaccg aagacgctgt agtagccttc
    23161 ttcggtcaac tgtgcggcgt ccatacgggt tggcggtgcc acccacaggc gggtcggcaa
    23221 ctgaccttta tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga tgttggtcat
    23281 gcaggaaccg ataaacactt cgtcgatctt ctcaccctgt accgcagaca gcggacgcgc
    23341 gtcatccggg tcgttcggag cacacaggat tggctcttta atatccgcca gatcgatgtc
    23401 gatcactgcc gcgtattccg catctgcatc ggcttccagc agctcaggat tcgccagcca
    23461 tttttccatg ccctgaatac gacgttccag ggtacgacga tcgccgtaac cttccgcgat
    23521 catccacttc agcaggacga tgttagagtt caggtattcg atgatcggtt ctttgttcag
    23581 cttgatggta caaccggcag cagaacgctc ggcggacgca tcggtcagct caaaggcctg
    23641 ctcaactttc agatccggca gaccttcaat ttcgaggatg cggccagaga agatgttttt
    23701 cttgcctttc ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg gaatggcgtg
    23761 taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga
    23821 ttccggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca gaccagaacc
    23881 cgccgggaaa gagataccga tcgggaaacg ggtatgggag tcacctccgg taccgacggt
    23941 atccggcagc agcatacggt tcagccagga gtggattacg ccgtcacccg gacgcagcga
    24001 cacaccgcca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg
    24061 cttcggatac gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg agaagcccag
    24121 gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct gagagcctac
    24181 agaagtcatt ttcggttcgc agtacgcgcc cggacgaatg cctttcacgc cacaggcacg
    24241 gcctaccatt ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg
    24301 acggaacaca tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg
    24361 cccgataatc agcggaatac ggccgccagc acgcacttca tcaatcagca cgtcggtttt
    24421 cagttcgaag gtcgccagca gttcgccggt ttcgtggtta cgcacttcac ctttgtacgg
    24481 gtaaacgtca atcacgtcgc ccatgttcag gttagagacg tcgacttcga ttggcagtgc
    24541 acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc
    24601 accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt
    24661 agtggcggat ttacgcgaag aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa
    24721 acctttctgt tgcagagctt cgatttgctt gatcggacca acaacaccag gctggtctgg
    24781 ctcaatacct tcacgggcgt ttttcagcat cgccaacgcg tgcagtggga tatccgggcg
    24841 tgaccacgca tccggtgccg gagaaaggtc atcggtgtta gtttcgccag tgactttgaa
    24901 gacggtaacg gtcagttttt cagccagcgc cgggcgattc aggaaccatt cggcatccgc
    24961 ccaggactgc ataacctgct tcgcatattc gttgcctgct ttcgctttct cttctacgtc
    25021 atagaagtta tcgaacatca gcagcgtgtg agaaagtgct ttggcagcaa taggtgccag
    25081 tttggcatca tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtgcc
    25141 cagcagttcg atggcttttt ccggagtcag cagaggggat ttggcttcgc ctttcgcgat
    25201 agcagccagg aagcctgctt tgacataggc ggcttcatcg acgcctgggg gaacacggtt
    25261 ggttaacaga tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac
    25321 aagtgcggcc atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc
    25381 agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt
    25441 gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt
    25501 accgcaacgg gcagaatagc aggattttga cgatgtgtta atctgtttac aaaaaagcaa
    25561 cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact
    25621 tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta gtcaaaattt
    25681 aaactaccgc ctctttatac tcggattcac agcacctgcg ggtggcagtt cgccgaccat
    25741 tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg
    25801 gcgctaattt gcagtgccgg gctttgtgcc gcctctaccg ggctatatat aaaaagccgc
    25861 acagtggaag cgcctgtgga accgcaatcg acacaactgg ctgtgtctga cgctgccgcg
    25921 gttacgtttc ctgcaacggt ttccgcacct cccgtaacac ccgccgtcgt caaatccgca
    25981 ttcagcactg cacaaataga tcaatgggtc gcacccgtcg cgctgtatcc cgacgcccta
    26041 ctttcgcagg tgctgatggc atcaacctat ccgacaaacg ttgctcaagc agtgcaatgg
    26101 tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc tgaccagccg
    26161 tgggacgcca gcgttaaatc actggtggcc tttccacaat tgatggcatt gatgggcgaa
    26221 aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cccagccgca ggacgtgatg
    26281 gactcggtac agcgattgcg gcaactggcg caacaaaccg gctcgctgaa gtcatcaacc
    26341 gaacagaaag ttattaccac aacgaagaaa gctgtaccgg taaaacagac agtcacggca
    26401 cccgtcatac catccaatac agttttaact gccaaccccg tcattacaga gccggcaaca
    26461 accgtcattt ccattgagcc cgccaatcct gatgtggtct atattcccaa ctacaaccca
    26521 accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca
    26581 gccggagaac cgtttgttga cagctttgta cgcggattcg gctatagcat gggcgttgct
    26641 accacgtacg cactattcag cagcatcgac tgggacgacg acgatcatga ccatcatcat
    26701 catgacgatg atgattatca tcaccacgat ggcggtcatc gtgacggtaa tggctggcaa
    26761 cacaacggcg acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat
    26821 cttactgata agaatatggc atggcggcac aatccaaact accgtaatgg tgtgccctat
    26881 catgatcagg atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagcgcc
    26941 acgcaattac ctgccccaac gcgcgacagc cagcgtcagg cggcagcaaa tcagtttcag
    27001 caacgaacac acgccgcacc agtcattaca cgagataccc aacgtcaggc agcggcacag
    27061 cggtttaatg aagctgaaca ctatgggagc tatgacgact tccacgactt cagccgtcgc
    27121 caaccactga cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc cgcctcgcct
    27181 gagcagcgcc aggcagttcg cgagagaatg cagactaacc cgcagatcca gcagcgaaga
    27241 gaggcagcgc gtgagcgcat tcagtccgcc tcgcctgagc agcgccaggc ggttcgcgag
    27301 aaaatgcaga ctaacccgca gaaccagcag cgaagagacg cagcgcgtga gcgtattcag
    27361 tccgcatcac cagagcagcg ccaggtgttt aaggaaaaag tacagcagcg cccactgaat
    27421 caacagcaac gtgataacgc ccgccagcgt gttcaatcgg catcacctga acaacgtcag
    27481 gtttttcggg agaaagttca ggagagccgc ccacaacgtc taaacgacag taaccatact
    27541 gtcaggctga ataacgagca acggtcagca gtacgcgaac gtctctctga acgtggagca
    27601 agaagactgg aaaggtaaat tgcagacgta aaaaaagcgg cgtggttagc cgctttttta
    27661 attgccggat gttccggcaa acgaaaaatt acttcttctt cgctttcggg ttcggcaggt
    27721 cggtaatgct accttcgaac acttctgccg ccaggcccac agactcgtgc agagtcgggt
    27781 gcgcgtggat ggtcagtgcg atgtcttcag catcacaacc catttcgatt gccaggccga
    27841 tttcacccag cagctcgccg ccgttagtac cgacaatcgc accaccgatc acacggtgag
    27901 attctttgtc gaaaatcagc ttggtcatac cgtctgcgca gtcggaagcg atagcacgac
    27961 cagaagcagc ccacgggaag gtggcggttt catagctgat gcctttctct ttcgcttctt
    28021 tctcagtcag acccacccat gcaacttctg gttcggtata ggcgatggac gggataactt
    28081 tcggatcgaa gtagtgtttc ttaccggcga taacttcagc ggcaacgtga ccttcgtgaa
    28141 cacctttgtg tgccagcatc ggttgaccga cgatatcgcc gatagcaaag atgtgcggta
    28201 cgttggtacg cagctgtttg tcaacgcgga tgaaaccacg gtcgtcaact tccacgcctg
    28261 ctttgcctgc gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc agcacggcgt
    28321 cgtaacgctg cggttcagcg ggtgcttttt tgccttccat cgtcacataa atgccgtctt
    28381 ctttcgcttc aacggcggta actttggttt ccagcatcag gttgaatttc ttgctgatac
    28441 gcttggtgaa gactttaacg atgtctttgt cagctgccgg gataacctgg tcgaacattt
    28501 caaccacgtc aatctgtgaa cccagcgcgt ggtaaacggt gcccatttcc agaccgatga
    28561 taccgccacc cattaccagc aggcgttctg gtacttcttt cagttccagc gcgtcagtgg
    28621 agtcccagat acgcggatct tcatgcggaa taaacggcag ttggatcggg cgagaacccg
    28681 ctgcaatgat cgcgttgtcg aagttgatca cggttttacc gttctcacct tcaacttcca
    28741 gggtgttagc cccggtaaat ttacccagac cgttgaccac tttgactttg cggcctttcg
    28801 ccataccagc cagaccaccg gtcagctgat tgatcacttt ctctttccag gtacgaatct
    28861 tgtcgatatc ggttttcggt tcgccaaaga cgataccgtg ttcagccagc gctttggctt
    28921 cttcgataac ttttgctacg tgcagcagtg ctttagaagg gatacagccg acgttcaggc
    28981 aaacaccgcc aagggtgttg taacgttcta cgattacggt ttccagacct aaatcagcgc
    29041 aacggaaggc agcggagtaa cctgcggggc ctgccccaag taccacgacc tgagttttga
    29101 tttcagtact catcatgacc tctatatatt tatctccggc gggtctgacg ttttttttat
    29161 gacgcccatt atccaccggg tcgttctatc cgtcggtatt ttacaaaatt gttaacaatt
    29221 tttaaacaac aaacggcaac cgatttgtct attcgctaat aacctcaata cattcatgag
    29281 attaccagaa aaaagccggc cgttgggccg gctcttttac ttacatcacc agacggcgaa
    29341 tgtcagacag cgtgttgtta atgatggtaa tgaaacgggc accatcagca ccgtcgatca
    29401 cgcggtggtc gaaggagaga gaaatcggca gcatcagacg cggcacgaac tctttaccat
    29461 tccacaccgg ctccatcgcg gacttggaaa cgccgaggat agccacttcc ggcgcgttca
    29521 caatcggcgc gaagtgggta gtacccaggc cgccgatgct ggagatggtg aagcaaccgc
    29581 cctgcatttc gcccgcagtc agcttaccgt cacgcgcttt cttagaaata gtcatcagct
    29641 cgcgagacag ctcgatgatg cctttcttgt tgacgtcttt gaataccgga acaaccagac
    29701 cgttcggggt atccaccgcc acaccgatgt tgatgtactt cttcagggtc agacgctgac
    29761 cgtcttccga cagcgaactg ttgaagcgag gcatctgctc aagagctgca gcaacggctt
    29821 tcatgatgaa gacaaccggg gttatcttca catccagctt acgtttcgcc gcttcttcgt
    29881 tctgctgttt acggaacgct tccagctcgg tgatatcggt tttgtcgaag tgagtaacat
    29941 gcgggatcat cacccagtta cggctcaggt tcgccccaga gattttctgg atacggccca
    30001 gttccacttc ttcgatttca ccaaacttgc tgaagtccac cttcggccac ggcagcatgc
    30061 ctgggatacc gccgccagtc gccgccggag ctgcttctgc acgtttgata gcttctttca
    30121 cgtaagcctg aacgtcttcg cgcaggatac gacctttacg gccagtgccc ttcactttcg
    30181 ccaggttaac gccaaactcg cgtgccagac ggcggatcag cggagtcgcg tgaacgtaag
    30241 cgtcgttttc agcaaattca gatttgcctt ccgcttttgc agccggagct gctgccgggg
    30301 cttcagcttt tgctgccgga gccggcgctg ccgcttcctg tttcgcagga gctgcagcag
    30361 gcgctgcgcc ttcaacttcg aagatcataa tcagcgaacc agttttcact ttatcgccaa
    30421 cgttgacttt cagttccttc acgacgcctg caaacggagc cggaacttcc atagacgctt
    30481 tatcgccttc tacggtgatc agtgactgtt cagcggcaac tttgtcgccc actttcacca
    30541 tcacttcggt gacttcaact tcgtcaccgc cgatatccgg aacgttaact tctttcacgc
    30601 cagctgctgg tgcaggagcc gctgccggag ccgcttcctg tttagccgcc ggagctgccg
    30661 cgcctgcttc acccgcgact tcgaagacca taatcagcga gccggtagac actttgtcac
    30721 ccacgttcac tttgatctct ttcacggtgc cagcaaacgg agccggaact tccatagaag
    30781 ccttgtcgcc ttctacggtg atcagcgact gttcagcttc aactttatcg ccaactttca
    30841 ccaggatttc ggtcacttca acttcgtcgc tgccgatatc cggaacgtta acgtcttttg
    30901 ccgctgcagc cgctggtgct gctgccggag ctgcttcttt cttctcttct gcctgagcag
    30961 gtgcagcgtc tgctgcaccg tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg
    31021 ttttatcgcc aacagagact ttgatctctt taacaatacc cgcctgcgga gacggaactt
    31081 ccatagaggc tttgtcgcct tctacggtga tcagcgactg ttcggcttca actttgtcgc
    31141 ccactttgac caggatctcg gtgatttcaa cttcatcagc cccgatgtcc ggtactttga
    31201 tttcgatagc cattattctt ttacctctta cgccagacgc gggttaactt tatctgcatc
    31261 gatgttgaat ttggcgattg cgtcagcaac cactttctta tcgatttcgc cacgtttagc
    31321 cagttcgccc agcgccgcaa ccacgacata agaagcatca acttcgaagt ggtgacgcag
    31381 gttctcacgg ctgtcggaac gaccgaagcc atcagtaccc agtacgcggt agtcgtcagc
    31441 cggtacgtaa gtacggacct gctcagcgaa cagtttcata tagtcggtag atgccactgc
    31501 cggagcgtcg ttcatcacct gagcgatata cggaacgcgc ggagtttcca gcgggtgcag
    31561 catgttccag cgttcacaat cctgaccatc acgcgccagc tcggtgaagg aggtcacgct
    31621 ataaacgtca gaacctacgc cgtaatcttt cgccaggatc tctgccgctt cacggacgtg
    31681 acgcaggata gaaccggagc ccagcagctg aactttacct ttgctacctt caatggtttc
    31741 gagtttgtag atacctttac ggataccttc ctcagcacct tccggcattg ccggcatgtg
    31801 atagttttcg ttcagcgtgg tgatgtagta gtaaacgttc tcttgttttt caccgtacat
    31861 acgctccaga ccgtcatgca tgatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga
    31921 gatacagttc gggatagtca gcgactgaat gtggctgtga ccatcttcgt gctgcagacc
    31981 ttcgccgttc agggtggtac gaccggaagt accgccgatc aggaagccac gcgcttgctg
    32041 gtcgccagcc gcccagcaca gatcgccaat acgctggaag ccgaacatcg agtaatagat
    32101 gtagaacgga atcatcggca gattgttggt gctgtaagag gtcgccgctg ccagccagga
    32161 acaacctgcg cccagctcgt tgatcccttc ctgcagaatc tgacctttct cgtcttcttt
    32221 atagtaagca acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat
    32281 accaatctga cggaacagac cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg
    32341 taccagacga tctttgatcg acttgttctt cagcatcacg ttcagagcac gaacgaaagc
    32401 gatagtggta gagatctctt tgctctgctc ttccaacagc gcgccgaagt cttgcaggct
    32461 cggcagctca agcttctcgg tgaagttcgg ctgacggctt ggcagataac cgtgcagttt
    32521 ctgacgctga gcgtgcagat aggtatgctc ttcagaacct tccgggaagg tgatgtacgg
    32581 cagtttttcg atatctgcat cagacaccgg cacattgaaa cggtcgcgga tatgacgcac
    32641 accgtccatg ttcattttct taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc
    32701 catgccataa cctttaatgg tatgagcaag gattactgtc gctttgcctt tggtttcctg
    32761 cgctttcttg aatgcagcgt agattttctt cggatcgtga ccaccacggt tcagtgccca
    32821 gatctgctcg tcagtccagt ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa
    32881 gtgttcacga acgtacgcac catctttcga tttgaaggtc tggtagtcgc cgtcaacggt
    32941 ttcgttcatc agctggatca gtttaccgct ggtatcttta cgcagcagtt catcccaacg
    33001 gctaccccac atcactttga tcacgttcca gccagcacct tcgaagatgc cttccagttc
    33061 gttgatgatc ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat
    33121 aacgaagacc aggttatcca gtttttcacg ggtagcgatg gtgatcgcac ctttggattc
    33181 cggttcgtcc atttcaccgt caccgaggaa cgcgtaaacg gtttgtttag aggtatcttt
    33241 caggccacgg tgttccagat atttcaggaa tttagcctgg taaatagcac caatcggacc
    33301 cagacccata gatacggtcg ggaactgcca gaattccggc atcagtttcg ggtgcggata
    33361 ggaagagagg ccattgccgt gaacttcctg acggaagtta tccagctgct cctgagtcag
    33421 acgaccttcc aggaaagcac gagcgtaaac gcccggggag atgtggccct ggaagtaaac
    33481 caggtcgccg ccatcctgct cgttgcgtgc acggaagaag tggttaaagc acacatcata
    33541 aatggttgcg gaagactgga aggacgccat atggccgccc agttcgaggt cttttttcga
    33601 cgcacgcaga accgtcatga tggcgttcca gcggatagct gaacgaatac ggcgttccag
    33661 ttccagatta cccggatact ccggttgttc ttcaacgggg atggtgttga tgtagttgct
    33721 gataccggtg cctgcggcta cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc
    33781 gatcagatac tgagcacgct caacaccttc ttcacggatg accgattcga tcgcctggag
    33841 ccagtcgcga gtttcgatcg gatccacgtc atttgggaaa cgttctgaca tgggttattc
    33901 cttatctatc taataacgtt gagttttctg gaacctgtcc cattgaactc tcgccggaaa
    33961 gctcaataag acaggttcta cgtttagttg ccgcgctttt atatgcgctt gatttacaac
    34021 atcttctgga taatttttac cagaaaaatc actaattctt tcgttgctcc agacgacgca
    34081 gagaacgctc acggcggctt tcttcacggc ttctgtcgag caaaatttct tcgataaagg
    34141 ccagatggcg atgcgatgct tcgcgcgctt cttccggctt accggccata atcgcttcaa
    34201 atatgcgggt gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt
    34261 cgaagttctg gcggacattc tgggccaaca tcggctccat acaccttagc agatgaagca
    34321 gaaccacatt gtgggccgct tcggtgacgg caatctgata ctggagtacg gcgtttgatt
    34381 ccgcgtccag atcgccagac tgctgcgcca gctctatggc gtggtggagt tcacggatgc
    34441 gttccttgtc ttcatcggta ctacgcagcg cggcgtaata agcggcgata ccttccaggg
    34501 cgtgtcgtgt ttcgagcaag tcatactgtg attcaggatg gtcggagagc agctctacca
    34561 gcggatcgct gaagctttgc cataggctgc tctggacaaa agtgccgcca ccctggcgac
    34621 gaagcaacaa gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga
    34681 cgtcaaactg ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc
    34741 cttcgaggat caaaaactcc agttgctgct caatcacatc ggagagtttt ggttggcgga
    34801 ttttgctgta ggccatgagt tcctgtctta agccacttgc cgaagtcaat tggtcttacc
    34861 aatttcatgt ctgtgacgct aaagtaacaa agtattcacc ttatgtccat acaggttttg
    34921 attgaaatca tgaaactgtg cacattttaa caacttgaca tatatagcgt
//
LOCUS       DAOFQD010000042        34657 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.042, whole genome shotgun sequence.
ACCESSION   DAOFQD010000042 DAOFQD010000000
VERSION     DAOFQD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.042"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(73..1326)
                     /gene="amiC"
                     /locus_tag="QBF46_002061"
     CDS             complement(73..1326)
                     /gene="amiC"
                     /locus_tag="QBF46_002061"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDQ6674243.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFAFSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1558..2889
                     /gene="argA"
                     /locus_tag="QBF46_002062"
     CDS             1558..2889
                     /gene="argA"
                     /locus_tag="QBF46_002062"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDQ6674244.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2951..4777)
                     /gene="recD"
                     /locus_tag="QBF46_002063"
     CDS             complement(2951..4777)
                     /gene="recD"
                     /locus_tag="QBF46_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDQ6674245.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQLDFTD
                     IEKRLLQSGKDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4777..8319)
                     /gene="recB"
                     /locus_tag="QBF46_002064"
     CDS             complement(4777..8319)
                     /gene="recB"
                     /locus_tag="QBF46_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDQ6674246.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFVTNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDKQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8312..11200)
                     /gene="ptrA"
                     /locus_tag="QBF46_002065"
     CDS             complement(8312..11200)
                     /gene="ptrA"
                     /locus_tag="QBF46_002065"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDQ6674247.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FNYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDIVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11376..14744)
                     /gene="recC"
                     /locus_tag="QBF46_002066"
     CDS             complement(11376..14744)
                     /gene="recC"
                     /locus_tag="QBF46_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDQ6674248.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKSHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYECNMMVCGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14757..15080)
                     /locus_tag="QBF46_002067"
     CDS             complement(14757..15080)
                     /locus_tag="QBF46_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDQ6674249.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15065..15472)
                     /locus_tag="QBF46_002068"
     CDS             complement(15065..15472)
                     /locus_tag="QBF46_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDQ6674250.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLCLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15469..16032)
                     /locus_tag="QBF46_002069"
     CDS             complement(15469..16032)
                     /locus_tag="QBF46_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDQ6674251.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16023..16493)
                     /locus_tag="QBF46_002070"
     CDS             complement(16023..16493)
                     /locus_tag="QBF46_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDQ6674252.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16678..17472)
                     /gene="thyA"
                     /locus_tag="QBF46_002071"
     CDS             complement(16678..17472)
                     /gene="thyA"
                     /locus_tag="QBF46_002071"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDQ6674253.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17479..18354)
                     /gene="lgt"
                     /locus_tag="QBF46_002072"
     CDS             complement(17479..18354)
                     /gene="lgt"
                     /locus_tag="QBF46_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDQ6674254.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18505..20751)
                     /gene="ptsP"
                     /locus_tag="QBF46_002073"
     CDS             complement(18505..20751)
                     /gene="ptsP"
                     /locus_tag="QBF46_002073"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDQ6674255.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDIISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARAKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20764..21294)
                     /gene="rppH"
                     /locus_tag="QBF46_002074"
     CDS             complement(20764..21294)
                     /gene="rppH"
                     /locus_tag="QBF46_002074"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDQ6674256.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21979..22668
                     /gene="mutH"
                     /locus_tag="QBF46_002075"
     CDS             21979..22668
                     /gene="mutH"
                     /locus_tag="QBF46_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDQ6674257.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22737..23450
                     /locus_tag="QBF46_002076"
     CDS             22737..23450
                     /locus_tag="QBF46_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDQ6674258.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23588..23806
                     /gene="ygdR"
                     /locus_tag="QBF46_002077"
     CDS             23588..23806
                     /gene="ygdR"
                     /locus_tag="QBF46_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDQ6674259.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23914..24954
                     /locus_tag="QBF46_002078"
     CDS             23914..24954
                     /locus_tag="QBF46_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDQ6674260.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPTGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24986..26179)
                     /gene="lplT"
                     /locus_tag="QBF46_002079"
     CDS             complement(24986..26179)
                     /gene="lplT"
                     /locus_tag="QBF46_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDQ6674261.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26172..28331)
                     /gene="aas"
                     /locus_tag="QBF46_002080"
     CDS             complement(26172..28331)
                     /gene="aas"
                     /locus_tag="QBF46_002080"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDQ6674262.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDELGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28917..29948
                     /gene="galR"
                     /locus_tag="QBF46_002081"
     CDS             28917..29948
                     /gene="galR"
                     /locus_tag="QBF46_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDQ6674263.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDDDLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29955..31217)
                     /gene="lysA"
                     /locus_tag="QBF46_002082"
     CDS             complement(29955..31217)
                     /gene="lysA"
                     /locus_tag="QBF46_002082"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDQ6674264.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31339..32274
                     /gene="lysR"
                     /locus_tag="QBF46_002083"
     CDS             31339..32274
                     /gene="lysR"
                     /locus_tag="QBF46_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDQ6674265.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32261..32953)
                     /gene="ygeA"
                     /locus_tag="QBF46_002084"
     CDS             complement(32261..32953)
                     /gene="ygeA"
                     /locus_tag="QBF46_002084"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDQ6674266.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33082..34500)
                     /gene="araE"
                     /locus_tag="QBF46_002085"
     CDS             complement(33082..34500)
                     /gene="araE"
                     /locus_tag="QBF46_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDQ6674267.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8465 a         9518 c         8585 g         8089 t
ORIGIN      
        1 ttattgaaca ccctaacggg tgtttttttg tttctggtct cccataaaaa agcgccattc
       61 agcgcctttt tatcatcccc ttctcgccag cgtcgcccca tcggcaaaat acgctttaat
      121 ccccgcaaga atagactccg caacttcctg ctggaaagtc gccgttttca gtttacgctc
      181 ttcctcaacg ttactgataa acgccgtttc gaccagaatg gagggaatat ctggtgcctt
      241 tagtacggca aacccggcct gttcaacttg atttttatgc agcttgttga ttttaccgag
      301 cttattcagt accgctttac caaacttcag gctgtcggca atggtcagcg actgtaccat
      361 atcgaacatg gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc
      421 cgaggcgttc tgggtttgtg ccagatattt tgccgcagta ctggttgcac ctttggttga
      481 gaacgcaaac acagaggaac cgctcggctg acgactggta aaggcgtcgg catggataga
      541 gacaaacagg tcagcacgct gtttctgggc ttttgctacg cgcacttgca atggaatgaa
      601 gatgtcttca ttgcgcgtca tgtacacctt catattgccc tctttctcga tcagagagcg
      661 caaacggcga gctatttgca ataccacatc tttttcgcgc gttttgtatt tccccaccgc
      721 accggagtct tcgccaccgt ggccagggtc aagcataatg acaatcggac gatcgcgccc
      781 tgctttaccc ggttgtggac cactttgcgc tggcggcacc tgcttttcga ggtcgccttt
      841 gttgtaatcc tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata
      901 gaggtccatc accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg
      961 ttttacgttt tgctttaatt caaaaaccat ccgtacggtt tgcgggtcaa attgcccgac
     1021 gcgcgccgac ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac
     1081 cgagttcagg tttacatctt cgatatccac caccacgcgt tcagggttac tcaacgcgaa
     1141 ctgcttatat ttcagctgac gatttgattc caccgtcacg cgggtgtagc tggacgcagg
     1201 ccagacgcgc accgccacga cctggctgac cgcagccaga ctgacctgac ttacgctcaa
     1261 tagccacatg gcacccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc
     1321 tgacatgcct ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg
     1381 accggaaact ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt
     1441 tatattcacg gcattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct
     1501 gcgaaaaaac agaataaaaa tacactaatt tcgaataatc atgcaaagag gtgtgccgtg
     1561 gtaaaggaac gtaaaaccga gttggtcgag ggattccgcc attcggttcc ctatatcaat
     1621 acccaccggg gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat
     1681 ttctccagta tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg
     1741 gtctatggcg cacgtccgca gatcgacgca aatctggctg cgcatcacca cgaaccgctg
     1801 tatcacaaga atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg
     1861 ggaacattgc aactggatat tactgctcgc ctgtcgatga gtctcaataa cacgccgctg
     1921 cagggcgcgc atatcaacgt cgtcagtggc aattttatta ttgcccagcc gctgggcgtc
     1981 gatgacggcg tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgatc
     2041 catcgtcaac tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact
     2101 ggcgagagct ttaatctgac ctcggaagag attgccactc aactggccat caaactgaaa
     2161 gctgaaaaga tgattggttt ttgctcttcc cagggcgtca ctaatgacga cggtgatatt
     2221 gtctccgaac ttttccctaa cgaagcgcaa gcacgggtag aagcccagga agagaaaggc
     2281 gattacaact ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc
     2341 gtgcgtcgct gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc
     2401 tcacgcgacg gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca
     2461 acaatcaacg atattggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt
     2521 ctggtacgcc gttctcgcga gcagctggag atggaaatcg ataaattcac cattattcag
     2581 cgcgataaca cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg
     2641 gaaatggcct gtgtggcagt tcacccggat taccgcagct catcacgggg tgaagttctg
     2701 ctggaacgca ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc
     2761 acgcgcagta ttcactggtt ccaggaacgt ggatttaccc cggtggatat tgatttattg
     2821 cccgaaagca aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat
     2881 ttagggtaag cgatgaaaat cgtcggatgc gacaagcgta acactcgtac gtcgcatccg
     2941 gcaattacgt ttattcccgt gaactaaaca acgccgccag accactgcgc cgctcagtac
     3001 gagtggcgat tgccgcactt aatatgcgct catcggcata cagcgacaga cggcgacgcg
     3061 cgcgggtcac cgcggtataa accagctctc gcgttactac cggcgtgcgt tggctcggca
     3121 aaatcaacgc cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg
     3181 tagtttcgtg ctctggcaag cgactcggtt gcacagactt aatattgccg tccggcatcg
     3241 caaaccagac gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca
     3301 acccaagcgc gctgtcatta cgggcaatca tcaccggtcg accttcgtac caacgagagt
     3361 gcggatgacg atgaattttg cgcttctgtt gcataaactg ctcaattcgc tcattcagtc
     3421 cagccacgcc aaacggccct tcccgcaggg cgcacaaaag ctggtactca ttgaacgcct
     3481 gaatgattaa atccggctcg gcacgcgctt gcagcagatc cagataacgt ccgtaacccg
     3541 caagagcttc ctcaagcatc gcaatataat ctttgccgct ctgtaaaagc cgtttttcga
     3601 tatcagtaaa atccagctgg aaaacggttt tcactgccgt tttatcacca cggttgatcg
     3661 ccgcagctaa ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc
     3721 agagactgtc gcgcaaagat gccgcttctg tgccagttcc tgccggaacg tgagttcccg
     3781 tcaggcggct tagctgcctg gcacgctcgg cggtaaagcc cgcgttggca taagcgcaga
     3841 tatcgcccag cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga
     3901 tcactcgcgc atgatcgggc aaggcgtcga tcagtctcga catcataggc agatcgatca
     3961 ttgacgcttc atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta
     4021 aacgctggct acccggctgc gcgcccagca atcggtgcaa agtgctggca tcttccggaa
     4081 tgcgtttctt ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg
     4141 ttaagcgcgc ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt
     4201 cggccatttg aattaacgct gccagcaact tcgctacggt ggtcgtttta ccggtgccag
     4261 ggccgccgga aatcaccgag atccgccgcg tcagcgccac tgccgccgca actttttgcc
     4321 agttaatttc atcgcttact ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat
     4381 caacctcaat ggcatgattc acttcgttga aaaagcgcgc taccgtccgc tcgttacacc
     4441 acatgcgatt caaataaaga cgatcgccac agaggatcat cggcgtgggt tcatctcccc
     4501 ggctgaccgc ttgtgaagcc agcaagcatt cttcccaatt ttgtagctca ccgatttcac
     4561 tgacacaggt cgccaacagc ggatgcgacg cctcgttatt ttccagtcgt gaaagcggca
     4621 aacagacgtg tccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag
     4681 gatgttcatc tcccgccacg gtcagggcaa attgcacatc cagcgggcgt agctgtttgt
     4741 gctccacagc ttccagtaat tgcttttgca atttcattac gcctcctcca gggtcatacc
     4801 ggcaaacatc tcatccatca ggtcaatcaa cccggcgttg ggtcgggttg tgtaaatccc
     4861 ctgttgcgga tgttctttat caacaccacg caggaacaga taaataacgc cgccaaagtg
     4921 gcgctcatag tcgtaatcag caatgcgatg gcgcagataa cgatgcagcg ccagggtata
     4981 aagctgatat tgcagatcat agcggtgtgc ctgcattgcc gctgccatag cctgttgggt
     5041 gtaagccgaa ctgtcttcac ccaaccagtt ggatttatag tcgagcaggt aataacgccc
     5101 ttcgtggcgg aacaccaggt cgataaagcc ttttaacatg ccacgtacct gcatgaactc
     5161 cagcggcggg cagccagcgg atagcgggtc aaactggcga attagtgcat caagctggct
     5221 ggcgatgagc ggttcactaa tcggcaggta aaactccatc tccacctgtt tatcgcggtc
     5281 ggaaagctga ttcaggctaa cacccgtttc attgagaggt gcctggagga cagccgtgat
     5341 ccactcggtc aacactggtt cccactgcga ttcaaagccg ccgagttcca gtttttcctg
     5401 tacccagttc gggtcaaccg gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa
     5461 tgtccctggt gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac
     5521 gctgaccacg cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg
     5581 accacgctgc tgcaaaccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg
     5641 tagcgtcctc gcattcagtt ctgcagttaa agcatcatta acctgccagg gctgtttatc
     5701 acccgtttgt gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg
     5761 aagccctgcc gcatcttgcg gttccccttt ttgcagcaaa cgcccgagcg cactttggtg
     5821 gacgtcggtg tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact
     5881 gcaatgccaa accgaacgtg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg
     5941 ttcggcctcc gcgaggtcga cgctttctgg cgcagcatta agatccagaa ctgcctcaaa
     6001 cgagtggcga tcgtgataaa acgcctgctc ctggacgcgg aaattggtga caaacggcag
     6061 ccagaccaat ggatattcca gccctttcga tttgtggatc gtgacaatct gcaccagatg
     6121 tttatcactt tcgagacgca tttgttggct ggaggcatta ctgtctggct cgaggatatg
     6181 ttgcgataac cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgtag
     6241 cagttcgctg atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa
     6301 gttttcagca atgttacgcg ccgacatcag cgcccgcagc atcggcataa cgccacgttt
     6361 gcgccagatt tgccgataac catcgaactc ttcgactacc acatcccacg catgttcgtc
     6421 attgttcagc gtttcgatat ccagcgcgtt cagccccatc attgacgttg ccagcgcact
     6481 acgcagggtg ttctcacgtt cgggcgtcat caccgcctgc aacaaccaaa gcatttcctg
     6541 cgcttccaga gtttcaaaaa cactgtcgcg gttcgaaagg taaacggaag ggatttccag
     6601 caacgttaag gcgtcgcgca cctgggcggc ctcctggcga ctgcgcacca gcacactgat
     6661 gtccgaagca cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg
     6721 tccggcttgt agccagtcgc ggatttgcgc agcacatacc tgcgccatgg tactttgata
     6781 gtcgccaacg ccgcagcttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc
     6841 acctttaaac acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg
     6901 tatttcgcga aacatgaacg cgtcatcagt ctggctgaaa agcttgttca cactgttcac
     6961 cattcctggt gcggaacgcc agttggtgtc taaagtgtag tgggcgtgaa cttcgctacg
     7021 cgccttcata taagtgaaga tatccgcacc ccggaatgca tatatggcct gtttcgggtc
     7081 gccaatcagc aacaatgcgg tttccggctg atggtgccag atacggcgaa aaattcggta
     7141 ctgctggggg tcggtatcct gaaattcatc gatcattgcc accgggaatc gcgtacggat
     7201 cgccgctgcc aacacctcac cgctttcgct acgcagcgcg gaatcgagcc gacttaacat
     7261 gtcatcaaaa cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc
     7321 agccaatgcg cgggtgatca ccagatcgcg gatcgacaat ggttctgcaa gcagttgatc
     7381 gatcgcctca aacagtggat gtcgcggggt ttcccccccg gctttcgtgc gatcttctaa
     7441 gaaacgctgg gagaattttt ccagcgactc cggcaactga taactgttgg tctcttcttc
     7501 tgcccaggcg ctgatcttgt cgatccattt agcctgattg ctacggttaa acttgcgtcg
     7561 atcaatacca gaagattcga tcagcgcatc cagttcaccc actgcgtcgc gccactgctg
     7621 ttttaccgta gcaatacgcg ccacaatttg cgcgtggcgg gaagccagcg tttcatcatc
     7681 gggcggcggt gctttgataa ccggcgcttc gccttgcaga taacgattaa tatcgcgcag
     7741 caacgcctgc ggccctttcc aggtttcaaa gacgacctgg gctatttcac gcggcagcgg
     7801 gtagcagtgg cgacgccaga aatcggcgca ggcctggtaa cgtagcagag actcatcttc
     7861 aatcagctgc tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg
     7921 gcaaaaaccg tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa
     7981 ccactgcgcg gcttgcgctt tatcgtcgat ctcttccagc agacgttcgt acagtggatt
     8041 gtcggtggtt tcacgcagac aggcgatgcg caactcatgg atattgctac ggatacgacc
     8101 acgcaattct gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacggtcag
     8161 cgggcgggga aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat agagcgccgc
     8221 aatcgtaaag gttttgcctg tgccggcaga ggcttcaatc aggcgctcac cctgtaaggg
     8281 caagcgcaaa ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca
     8341 ttgtttgctg caacgcgctg acgttctccc acactttcca gccttcaggg tgtacatatt
     8401 ctgctttccc attctggctg ccggaaatct gcgacaggat agccatgcct tgcggctcga
     8461 ctaccgcctg atggaagaaa tcggccagtt tttgcggcgt cagcagttta atctgggcca
     8521 cgattttatc acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttcg
     8581 atgcttcttc gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga
     8641 tttgcgcaaa ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg
     8701 ccttgtaacg ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca
     8761 tgccccactg acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt
     8821 cggtacgcaa ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg
     8881 ctgagctggt gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg
     8941 tgctgttacc ggctttttca aagatgacgg attgtttttt atcgaccact atatctttgt
     9001 ttcgacacca ctctgaacca tcagcgccca actgtttttg cacatcgcgt gccagggttg
     9061 ttgcctggtc ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cccgatttta
     9121 aggcgtcgcg ataggccagc acctctttca acgtaatgga gggcaaaatt ttgcgccgtt
     9181 catctcgcga gaagtaaggt acctgggaga gcatctgcgc gggcataatc gcctgctcga
     9241 acgctttgcc cttttctgcg gaatccatca tctggttata ccaggacttc gcctgctcaa
     9301 gctgatcttc cgtagcggta tagttaaagt agccctcgag caatgcctgg aacagctgcg
     9361 gcagacgctg ggtgtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa
     9421 aacttatgcc accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat
     9481 aatcattgag cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca
     9541 aaatcaggct gacatcggct ttgggctcgc tggcaaaata acggcttggc gcatacacta
     9601 ctcgcagatt cgactcatca acaatcagct ctggatggtc atatttcttc tctgacttaa
     9661 tcagcgagaa atcgtcagga atataggggt taagctccgg taaggagagc gcaatattgg
     9721 cagctttttg ctgccagtca gcgaaagttt gtgcgctaat tttatcgacc tgatacggcg
     9781 catcgacaaa gtaagccgtt ttattgtgcg gctctttcgg gctgatatac cagatacgcg
     9841 cattctgcgg cgtcatcatc gccagacgtt cttttactgc tttagcatcg taccgatcag
     9901 caatattgac tgcatccagc gtatgctcaa caggaacgcg aatcatggta tctgccagcc
     9961 attcgacgta atccatatca cgggtgattg acggataacg gaagtcgata tccagcacat
    10021 tcgccagttc atcgaagtat tgtttatcga tccctttttc acgtaacaga ttgaggtagc
    10081 taaaaatggc cgccacaacc tgatcgcgat tcgccagacc tttatcggtt aaagacgcag
    10141 agatcgctaa tacaccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct
    10201 caactaaccc ctgcttttgc agccagtcag aaagtgtacc tgggctgcga ttgccaatca
    10261 gataggtaat caattcatcg gttttactac ggaacttcgc tgagttgtta tcgatgcgaa
    10321 actcaacgcg caacacttta cgcggcagcg cagggacgta atgaatgata atgccctttt
    10381 gcgcgtcggt gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc
    10441 gaccaaaggt gtccgccgcc atttttgcca actccggcag cggtttatta ctgtaaataa
    10501 ccgccttcat caaattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca
    10561 ccggattacc aggtttgtcg cttaaagttt cgaggttacc accagaaaac tttgagccgg
    10621 ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac
    10681 gcgccatggt taattcagcg ttcactgcat tacgctcacg ttcggcgtat ttcttgtcga
    10741 gcaagggttc tgcaatagca tcggccaggc ggtctaccgc accgggcaag gcgtcgttct
    10801 caacttccag atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt
    10861 gcattttgag atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac
    10921 tcatatgttc aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa
    10981 cgggcaccac caacgccgat agcgatttaa ctgcctgcgg atcagaaacc agcaagacca
    11041 ccataccgtt atccagacgt atagcctgat actggcggtt atctttatca cttttacgga
    11101 tggtttcctg aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa
    11161 ctaacaacaa taatgctttg aaccaggtgc tgcggggcat tcacggacct cataatcaac
    11221 tttattttct ggccagattc aacaggttaa cgcaaatcat ctgccagaat ttaatcttgt
    11281 gctgcacgag tcagcctatg tctatatatc catcagtccg tgactggtgc gcatcataat
    11341 gtaagcgaat gaattgcgca atttttatac agcactcatg actgattaaa gcgaaacagc
    11401 ggtaacagga aacgttgcga ctgttcaacg atggcctcca ttgtctctgg tgttaattgc
    11461 cgccagagcc gttgatacca gatatcatca ccttcgccac acaccatcat gttgcattcg
    11521 taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca
    11581 tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc
    11641 aatggcgcgg acattccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc
    11701 tgttcggctg caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt
    11761 tcaccattac caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgg
    11821 gccacactta ataaagaggg acgccagcgc aacaggccat ccggctgcac ctgcggcaac
    11881 cagccagtta tctgcacacc gttgcaggcg agatcaattt ccatactctg ccccggctgg
    11941 cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa
    12001 atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcggcg gaacaagcgt
    12061 tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta
    12121 agcccttcca gaataaatgg ctcggtgtcg gggatttcgc tgtcttcggt acggaagttc
    12181 acctgcaaac gcatctggaa aaatgcccgc accggatgtg cccagaatcg ttgtagcgtt
    12241 tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct
    12301 ttaccagcct ggctggccgc aggtagccat tcacgagcat aactttgtcg ttcgcctggc
    12361 tggtagtttt gcggatcaaa cggcattcgg gtatgaaggc aagtaagatg cgattttacc
    12421 cttgcctcgc tttcatcaca gttgagcgcc tcatcgcccg gtagataatg actttgtccg
    12481 atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg
    12541 gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac
    12601 agatagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg
    12661 tcaaagccca atggcgcaag ctggcgtgga taaacgccgt cgttcattcc cagcaggcaa
    12721 accactttga atgggatgga acgcattggc atcagagtac aaatgttaac cggtccggcg
    12781 agaaaacgct gactgatacg ttcctgatcc aggcgctgtg ccagttcatc acgcaatagt
    12841 gacagcggca ccgcgtcgcc atactgcgca cctaaacctt cggcgataat cgcctgccat
    12901 tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gaaggcgttg
    12961 agcatatcgc gacaaaccgg caaccactct tccagcggac gctcctgcgc cagcccgcga
    13021 cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag
    13081 ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg
    13141 tagcccagca acatacgcgt caggccaaac cgccaggtgt gttgaccggt ggcggggagt
    13201 tccagctcgc gaacgttatc gtcatctatc ccccagcgaa tgccggattc gttgacccac
    13261 tggcgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca
    13321 tccagcagcg ccagcacatc ctctgacaca aagcgactgt caggcagtga taacaggctg
    13381 ataaacgctt ccagtaccgg atgtgactgc cgcgcccgac ggtcggaaat ggcgtaaggc
    13441 aggtaacgat ccgcaggtgc gctaccaaac acagcctgaa taaacggact gtagctgtcg
    13501 atgtcagcca ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ctccagcatc
    13561 gccagcaagc gatcgtgtaa aacttcaact tcacgctgcg ggctatggca aacgtggaag
    13621 gtgatactgc tatccagtgg atcaagcggg cgtttgttat cgctacggga aaactcttcg
    13681 atgttcacac ccgcaacggc gcggttttcc agttccagaa tgtcagactg aatgttatgc
    13741 agcaggttgt ctggcgtcac atcgacaaaa gcgtccagct cctggctgct ctccaggtca
    13801 gaaaggagat aaatgtagtc gcgcccaagc ttcccccatg aggccagcag cgggttgccg
    13861 acatcctgtt caccatcgct gttaaagagc tgtccggcat tttcgctgtc gcgaaatagc
    13921 ggtaattcgc gatcttcaaa actgtgtcgg cgctggcgag tcagtagttt cgccagataa
    13981 gcaggatctt taatatcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt
    14041 tcaatatgtt tacccagcgc ctggagcgcc tggagataaa caggcggtaa cgcggaaata
    14101 ccgcatataa agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt
    14161 tcgataaagc gctgatagag attggcgcgg tgccaacgcg gttgtccgag ttcatgggta
    14221 tattccacca gcgccttcca caacggcgct tgccaggcct gtgcttctcc cagcccttca
    14281 accaaatgtc ctgtttccca ctgtgccagc cactccgggc gatagaccag atactggtca
    14341 aacaggtccg ctgcttttga ggaaagctgg aacagttttc gcttgtcgct atcgtcagtc
    14401 agataatgcc gcaacagggt aaagtcttcg cgatccagca attgcggcag cagagtcatc
    14461 agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatctc cggtaacacc
    14521 cggacgaaca tatcccagat aaagctcgct ggcagcggaa aatcaatgtt tgccgcaata
    14581 ccaaactttt gcgacagggt catttgcagc cactgcgcca taccggtact ttgcaccaga
    14641 atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac
    14701 gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac
    14761 tgacgattcg ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg tgaaactagc
    14821 gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac ctgccagttg
    14881 gcaggtggcg aaatcgcgcg cagttgcgtt tgctgccaac catgccgcca gagctgttgg
    14941 tactggtttc tgctggcgag actgttcatt aatgttcgct ggatacccga taacgcagtg
    15001 acaatcatca ccatcagcac catcgcaacc attacctccg gcaggctaaa gccttgttga
    15061 ttcttcaggg aagctgacat aacgccctct ctttcagcgg acaaaaatcg ctccagccgc
    15121 gtggcgaaaa aacaatgttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt
    15181 cataaccggc aaccaataag gcttcattat ctgccagtaa acacaaacaa acccgggcat
    15241 cggttccagc gtactgcgag cactgaactg cgggctgcgt ctgccaggag tgcattttcc
    15301 cccacgccag cgccgactga acgatggcct ggcggcgcaa tgactgactt tccatgctca
    15361 cgcgagaggc aaaactgcga tcctgctgac tcattccttg caatagcaag ctacccaaaa
    15421 ccagcagcat cagaaccaga gccagtgacg aaacaccttt ttcgcggttc acaggttgaa
    15481 tcctgtcacg ctatagctgg catccaccac ggtttgcggt tcagacttac tggcagcacg
    15541 catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt
    15601 gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt
    15661 cgcaccgcgt agcgtttcca gcacatgctc cttcagacga aatccaatct ggtcagactc
    15721 ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca
    15781 gtcaccctgt ccgacaattt ccagcccttc gccggtacag ctgccatgac aataacccgc
    15841 cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagcgccag
    15901 cttacgggtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg ccgcccccag
    15961 caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc
    16021 ttttacaggc atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa
    16081 accaccagcc accactcgcc cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta
    16141 ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag
    16201 cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tggccccagc agcggaactc
    16261 acaaggcacc ataacgtccc ctccctgata acgctgatac tgtggtcgcg gttatgccag
    16321 ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccatagc
    16381 cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa
    16441 atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca
    16501 gtatggagca aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg
    16561 gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg aagaatttaa
    16621 cggagggtaa aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta
    16681 gatagccacc ggcgctttaa tgcccggatg cggatcgtag ccttcaatct caaagtcttc
    16741 gaaacggtag tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg
    16801 cggttcgcgg cttaattgca gatgagtttg atccatatgg ttgctgtaca gatgcgtgtc
    16861 gccaccggtc cagacaaaat cacccacttc cagatcgcac tgctgcgcca tcatatgcac
    16921 caataacgcg tagctggcaa tgttgaacgg caggccgagg aagacgtcac aggagcgctg
    16981 ataaagctgg caagagagtt tgccgtctgc cacatagaac tggaagaatg catggcacgg
    17041 tgccagcgcc attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcgcga
    17101 atccgggtcg tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc
    17161 tggcgttggc caggcgcgcc actgtttacc atacactggc ccgaggtcgc cgttttcatc
    17221 ggcccattcg tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc
    17281 ctgcagaaac cacagcagtt catggatgat ggaacgcagg tggcaacgtt tagttgtcac
    17341 cagcgggaat ccatcttgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc
    17401 ggttccggta cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa
    17461 atactgtttc atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac
    17521 catcatgatc acacccgcga caatcatcgg gatggaaaga atttgcccca tgctgatgta
    17581 ctgcacccag gcaccggtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg
    17641 aaacgcgccg taaccaatca ggaacaaacc tgagacagct cccattgggc gtggtttacg
    17701 aatatacagg ttgaggataa taaacagcac cacaccttcc agcagcagct cgtaaagctg
    17761 tgatgggtgg cgcggcagca caccgtaagt gtcgaaaatg gattgccact gcgggttggt
    17821 ttgcagcagc aaaatatctt ctgtacggga gccagggaac agcatggcaa acgggaagtt
    17881 cgggtcaacg cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag
    17941 accaaacgga atgagtggtg cgataaaatc agagacctgg aagaaggaac gtttagtacg
    18001 gcgggcgaag ataatcatca ccacgataac gccaatcagg ccgccgtgga aagacatgcc
    18061 gccgtcccag acacggaaca gatacagcgg atcggccata aactgcggga aattgtagaa
    18121 cagaacataa ccaatacgtc ccccgaggaa gacgccgagg aagcccgcat agagtaagtt
    18181 ttcaacttca tttttggtcc agccgctgcc cggacgattc gcccgtcgtg ttgccagcca
    18241 cattgcaaaa atgaaaccca ccagatacat caggccgtac cagtgaagcg ccacgggtcc
    18301 tattgagaaa atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca
    18361 ccacaagttc ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt
    18421 caggcgctcc aaaggtgcga ataatagcac aaggggacct ggctggttgc cggataccgt
    18481 taaaagatat gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccgcg
    18541 acgctccata aaggctgcaa cctgatggcg aacttcggtc gccagttgcg cttccagact
    18601 acgctgcgca agattttctg cttcggcata atcaatgcgc cgcagcaggt attttgcccg
    18661 cgctacagaa cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac
    18721 gcacatggga tcgcccgcca tttcaccgca caaacggaga tcgattccat gtatttccgc
    18781 ttcccgggcg atcatcgcca gagctcgtaa cattgcagga tgaagactgt cataaatgtt
    18841 cgccacccgg gtattgttgc gatcaacggc cagaatgtat tgagtcagat cgttggtgcc
    18901 aacagagatg atatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggcac
    18961 ttccagcatg atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc
    19021 acgtccggca cgttcaatca ggcggcgtgc ttcatcaact tcatcgaggc ttgtgaccat
    19081 cggcaacaga atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg
    19141 gatcaagaag atctccggct gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg
    19201 attctcttcg ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg
    19261 caaggtgacg ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc
    19321 ttcttccgac gggaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagaccgat
    19381 gccatcaata cggctgccca gtttttcttc atgttccggg cttaaaccag cattgagcat
    19441 gacttttata cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat cttccgccag
    19501 acggctaagc tcaatctctt cactaattag ccgctgatat tcttgcagca gtaccggctc
    19561 cggatcgacc agcaattcac cgcgatagcc atcaacgatc agcgtcctac gatgcagcac
    19621 cgaaggctga atatccgcgc ccatcacggt agggatcccc agcgcacgta ccatgatcgc
    19681 agcatgggag ttggctgcgc catctcgcac gacaacaccg actaagcgat cctggggcag
    19741 ctcagcaagc gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc
    19801 gttcggccct tgattagcgt catcaagatg aaacagcaat cgctgaccca gcgcacgtaa
    19861 atcgccagcc cgctctttga gatagttatc acttagcgcg gcaaactgtt cggcaaattt
    19921 ttcaatgacc gtttttaccg cccactctgc caccgagcct ttatcaacct cggcaaacaa
    19981 ttcgcgacgc agccgggtgt ccgaaagcag gtgcgagtaa agatcgaaaa tagccgccgt
    20041 ttctttttgt gcaccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc
    20101 ttccagcgcc ccggtcagtc gttcgcgttc cagagccgga tccagcgttg atgcctgata
    20161 cacctgttcc attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg
    20221 tgctgccggt aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaagg cagtcaactg
    20281 cgactgagaa agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact
    20341 ttcgtcatac tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg
    20401 ttgaataatt ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg aggggatgta
    20461 tttgaagctg gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac
    20521 caggccgacg atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag
    20581 cccccgggtc gccatcaggt agtaacaacg tcgatcatga tcggccaggt agaccgaaca
    20641 gacctcggta tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag
    20701 gcgtggtgcg ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt
    20761 gacttaacct cttttacgtc gataagcaga tgcgttttgt ggttttggcg tattttcctg
    20821 cagtgacatc accacactcg cgaactcttt cattacccta cggtagacat cacgtttaaa
    20881 tgacaccacc tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg
    20941 tgtactgctg gtttgcatat tgatttctgc atcgccgctc accagctgca agagaaacca
    21001 tttttgtttt tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa
    21061 tttgtagcgc aaccagttac gcgttgaagc aaggattcga acgtctttgc ggcttaatcc
    21121 tacttcttca aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttgattcc
    21181 gccttgcgga aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg
    21241 gcgattacaa atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact
    21301 acctcaaaat aaagctttat atacgaatga ttgtttcata ctccaggaag acggtaaacc
    21361 actctctgca gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt
    21421 tataaacaga ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt
    21481 gaagaactac ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatatagac
    21541 attttaaaat attcattatc agtcaatgca ttacatgtaa ttttagtaat gaaaaagagt
    21601 aattcgtgac ccaggtcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt
    21661 atttatccac agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt
    21721 cgcacgtcaa ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt
    21781 ctgtggataa catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacccgt
    21841 tgcagtgttg cgcaactcga ttaccggcaa cctaaaaagc aatataaatc agagaattga
    21901 acaacgcatg tggaaaaagt tacactgcga atattcggca cataattgct gtttgttttt
    21961 taatcaaggt atcatgacat gtcccaacct cgcccactgc tctctcctcc cgaaactgaa
    22021 gaacagttgt tagcgcaagc acagcaactt tctggttata cattgggaga actggcggca
    22081 cttgccgggc tggttacgcc agagaattta aaacgcgata aaggctggat tggcgtgtta
    22141 ctggagatct ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg
    22201 ggcgtggaac ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc
    22261 gtttgtgttg ccccgttaac gggcaatagc ggggtgacct gggaaaccag ccacgtgcgc
    22321 cacaaactca aacgcgtact gtggataccg gttgaaggcg agcgcagcat cccgctggcg
    22381 cagcgtcgcg ttggatcacc gttgctgtgg agcccgaatg aagaggaaga ccgacagcta
    22441 cgcgaagact gggaagaatt aatggatatg attgttctcg gtcaggttga gcggattacc
    22501 gctcgtcacg gggaatattt acagatacga ccgaaagcag cgaatgcgaa agcgcttacc
    22561 gaagccattg gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt ttatttgaag
    22621 aagaatttca ccagtgcgct actggcccgt cattttctga tccagtagcc atcgctttga
    22681 cctgccgctt tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt
    22741 tatttgcatg gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg
    22801 agatcgttct tgggatcgac aatattattt tcctttctct ggtggtggca aagcttccca
    22861 cagcacaacg tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg
    22921 cgctgctggc atcaatcgcc tgggttacgc gcctgacgaa tccgcttttt acaatattca
    22981 gtcaggaaat ttccgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga
    23041 aagccagcaa ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg
    23101 tttcatcatt cctcggcgct atcgtgcaga ttatgctgct ggatattatc ttcagcctcg
    23161 actcggtgat taccgctgtg ggtctgtcag atcacctgtt tattatgatg gccgccgtgg
    23221 tgattgccgt aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc
    23281 atccttcggt aaaaatgctg gcgctctctt tcctgattct ggtcggcttt accctgattc
    23341 tggaaagttt cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta
    23401 ttgcggttga aagcctcaac ctgattcgca acaaaaagaa cccgctctga tactccgtac
    23461 gctctcctgc gggagggcgt actcactcct gttaccctca cagtttaaga ttttcctgct
    23521 ttcaagatat ataacgtcgg tttataaaca gactattatc ataggtgagc caaatgagga
    23581 taaccggatg aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc
    23641 cgggtgttcc agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg
    23701 aaaacctgaa attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc
    23761 gatgcaaatt aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc
    23821 atccggctgg ccttaagatt tttctctttc ccttttccct tccctctgcc atttttatat
    23881 tccttatgtc gtgattataa aaaggaaacg gctatgcaat atcaccgtat accccacagt
    23941 tcgctggaag tcagcacgct ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa
    24001 gccgacgccc acgcacaact cgactatgcc gttgctcagg gcattaacct tatcgacgtt
    24061 gccgaaatgt acccagtacc tccgcgcccc gaaacgcaag ggttaaccga aacctacgtc
    24121 ggcaactggc tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc
    24181 ggaccgtcgc gcaataatga caagggcatc cgcccggatc aggcgctgga ccggaagaat
    24241 atccgcgaag cgttgcatga cagcctcaag cgcctacaga ctgattacct cgatctttat
    24301 caggtgcact ggccgcagcg cccaaccaac tgctttggca aactcggtta tagctggacg
    24361 gattctgcgc ctgcagtttc gctgctggat acgctggacg cactggcaga gtaccaacgc
    24421 gcgggaaaaa ttcgttatat cggcgtgtcg aacgaaactg catttggcgt aatgcgctac
    24481 ctgcatctgg cggacaaaca cgatctgccg cgtattgtca ccattcagaa cccttacagt
    24541 ctgttaaacc gcagttttga agtaggtctg gcagaagtca gccagtatga aggggtcgaa
    24601 ctgctggcct attcgtgcct gggtttcggc acgctgaccg ggaaatatct caatggtgca
    24661 aaacccactg gcgcacgtaa tacgctcttt agtcggttca cccgctatag cggtgagcaa
    24721 acgcaaaaag ccgtcgcggc gtatgttgat attgccagac gtcatggact ggatcctgct
    24781 cagatggcgc tcgcgtttgt acgccgtcaa ccgtttgttg ccagcactct gctgggcgca
    24841 accacgatgg agcagctgaa aactaacgtc gaaagtttgc atctggagtt aagcgaagac
    24901 gtattagctg aaattgaagc ggtgcatcag gtttatactt atccggcacc ataagattaa
    24961 cgccggttaa aaccggcgtt aaatattaat gacggcgctg ccagatccac agcgccgtta
    25021 ttgccagcgc aaacagcgca ccgaagccaa tgccaatggg cacgaccggg atgcctacca
    25081 ttaccgccag cgagtaaatg cccagcatca acaacatggc gctgttttcg ccaaggtttt
    25141 gtactgcaat cgcattcccc gccccgacgc tttttttacc ccgctcctgt agcaacgcat
    25201 tgagcggaac gacaaaaaag ccccccagca cgccaatcag catcaacaag gcataggctg
    25261 gcaacagctc gtgttgcagg gagaaaatca gtaccaccac gccaatcaaa atcccggctg
    25321 gcatacagcg tgacacggtt tccagcgtaa ctaacttcgc tgccgcacct gcgccaacca
    25381 cgataccaat cgctaccatc gcgttgagat aggtcggcgt agcgttatcg gtaatgccca
    25441 gcgccaccgg tacccacagc accaacagga aacgcagcgt gacacccgct ccccagaata
    25501 aactggtgcc caccagcgaa aaacgcgttt caccattgcg ccatagcgag gtgcaggcat
    25561 tcaggaaact gcgggtcatg ttgatgagat tccaggactg ccccggacgc gccgccgcca
    25621 gtttgggaat gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca
    25681 gggcgacgag gacatgccag tcagccagta caccaccggc tacggaaccg agcaaaatcg
    25741 ccgctatggt agaagcttcc atcaaaccgt tagcttttac taacttacta cccgtggtta
    25801 attcgccgag aataccgtat ttcgccggtg aataggctgc agcaccaaca cccaccagcg
    25861 tatagccgag aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc
    25921 cgttggcaaa catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg
    25981 gcgcaaaaag aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg
    26041 gatagaactg cgctttcagt aacgccagag tggcaaacag tagcgcgtta tcgccaaacg
    26101 cagagagaaa ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag
    26161 tgtgcactga ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca
    26221 aagtcaggtt tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt
    26281 acagcaagct ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt
    26341 tcgttatctg tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca
    26401 gtggcatgga ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac
    26461 accatttcgc ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc
    26521 agctcgtcaa aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga
    26581 acattctcgg cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc
    26641 attatgttcg gccctttcag ttgcaggcgt ccgccctctt cgataccagg gaccgacaac
    26701 aggcgcgcat ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt
    26761 acgttgatag aaacgacagg cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg
    26821 ccaaatttat cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc
    26881 acatagcgta gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa
    26941 gtcgaagtgc cgaacaacac ggtgcaactg cggtcataca ccaactccgg cacaatacgg
    27001 taatgcagcg ggcttggata aagaaacact tctgcacctg tgagcagtgg cgtaaacagg
    27061 cctaccgtca gcccaaagga gtgaaacagc ggtaacgccg acataaatcg atcgttggtg
    27121 gtgaagtcgg caatcgtttt aatctgctcg acattcgcca gaatgctttt atggctatgg
    27181 acgacgcctt tcggatggcc ttcagaaccg gaggtaaaaa ggatcagcgc ctcttcttcc
    27241 ggctgctgtt taacctgtgc cagacgcggc atcagcaaat gagcgaagat ccatactttg
    27301 tcggcagtgg tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt
    27361 tgctccggca gatgccagag tttgccttta tcgagaaact ggcgggaagt gaagatggtt
    27421 ttgatttcag ccgccgtaat agcactggtc agccctttta ccccggcagt gtagttcatc
    27481 attgcgggaa tgcggcgacg ggcgatggcc ccaaaaatca ctgccgcact gatgcctgca
    27541 ttgggcagca ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca
    27601 acaaacagcg ttttcgtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg
    27661 acacatttct tcccggctcc gaagcggtac attgcactca gtaaagattc gtacagcgtt
    27721 tcacgcgggc gcaccgccat tcgcgcttcc atcattattt gatgcagcat ttcgccagcg
    27781 attttgcgac ggtcacgggc acgcggcgca tccggcatct ccacctgcgt tggtggcaaa
    27841 atatgcagag taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag
    27901 tgcgtgagtt ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg
    27961 acaaaacccg cgccatcgta gattttcatc agcgagcctg tagtggtgat gcgtccttca
    28021 gggaaaatca ccactggtcg gccctgttcc accagacgta ccagatgttt aatagccata
    28081 ggttgcgtcg ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac
    28141 tgttggctta ttgaggtgta aacggcaaac actggacgca caggtaaaaa cagtccaagc
    28201 aaaatgccat caataaaaga gacgtgatta ggcgtaatta gaacgcgctc gcccttcagt
    28261 gcctgggtgt cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag
    28321 ctaaaaagca ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac
    28381 acgagataaa gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg
    28441 caggttggtg caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc
    28501 tctgggatct tgattgtggt ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa
    28561 tgaagggaaa tgcaacgagg tgtgtaaatt gtcggttact gttacagatt gatgaccggc
    28621 caaaaaaaac ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac ccgaagttga
    28681 cttcacctat caatacctct gggatcacca ctttagcaac ctaaagccaa acgccaccag
    28741 cggtcaatcg caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt
    28801 cttacttatt tgcgtttggc tcacattccc acgatgaaaa cacgccaccc cttgaaccaa
    28861 cgggcgtttt ccgtaacact gaaagaatgt aagcgtttac ccactaaggt atcttcatgg
    28921 cgaccataaa ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca
    28981 ttaataattc acctaaagcc agcgaagctt cccggctggc tgtgcatagt gcaatggagt
    29041 ctcttagcta tcacccgaac gccaacgccc gtgcgctggc gcagcagacc actgaaacgg
    29101 tcggtctgat cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg
    29161 aacaggtggc ttatcacacc ggtaatttct tattgattgg caacggttac cacaacgaac
    29221 aaaaagagcg tcaggctatt gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc
    29281 atgccaaaat gatcccggat gacgatttag cctcattaat gaaacaaatg cccggtatgg
    29341 tgctgatcaa ccgtatcctg cctggctttg aaaaccgttg tattgctctt gacgatcgtt
    29401 acggtgcctg gctggcaacg cgtcatttaa ttcagcaagg tcatacccgc attggttatc
    29461 tgtgctctaa ccactctatt tctgacgccg aagatcgtct gcaagggtat tacgatgccc
    29521 ttgctgaaag tggtattgcg gccaatgacc ggctggtgac atttggcgaa ccagacgaaa
    29581 gcggcggcga acaggcaatg accgagcttt tgggacgagg aagaaatttc actgctgtag
    29641 cctgttataa cgattcaatg gcggcgggtg cgatgggcgt tctcaatgat aatggtattg
    29701 atgtaccggg tgagatttcg ttaattggct ttgatgatgt gctggtgtca cgctatgtgc
    29761 gcccgcgcct gaccaccgtg cgttacccaa tcgtgacgat ggcgacccag gctgccgaac
    29821 tggctttggc gctggcggat aatcgccctc tcccggaaat tactaatgtc tttagtccga
    29881 cgctggtacg tcgccattca gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca
    29941 gcgactaacc gcagttaaag caattccagc gccagtaatt cttcgatggt ctggcgacgg
    30001 cgaatcaacc gcgcctgacc attatcaaac agaacttctg gtaacagcgg acggctgttg
    30061 tagttggatg acattgatgc gccatatgcc cctgtatcat gcagtaccag ataatcaccc
    30121 gccttcactt ccggcaaggc gcgggtttca acatttcccc cttcctgctg ggtaaagaca
    30181 tcgcccgatt cacataatgg cccggcgacg acggtttcca ccgttggcgc gtgttccaga
    30241 gaacgaccat cagctgccag ggcactgata tggtggtaac taccgtacat tgccgggcgc
    30301 atcagatcgt tgaacccagc atcaaccagc acaaagtggc ggctacccat ttgtttgacg
    30361 ctccgcacct gagtaattaa tacgccagac tgcgctacca ggaagcgacc cggttcaatt
    30421 tccagtttca cagggtggcc caaatggcgg gcgatttgct cacgcgcggc attccacaga
    30481 ccataataat gttcggtatc aaccgcctct tcaccctgtt gataaggaac agaaagccca
    30541 ccgcccgcag aaatagcctg taaatcctga ccgaattcga tgacctgacg caccatagca
    30601 ccacacacct gttccagatg ggcataatca acgccagaac caatgtgcat gtgaatgccg
    30661 accagctgca gatgatgacg ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag
    30721 ataccgtgct tgctgttttc gccaccggta ttggtttttt ggctatgtcc gtgaccaaac
    30781 cccggattaa cgcgcagcca tacccgatgc cctggcgaaa cctggcccag ttggtcgagc
    30841 atatcaacag aacccgcatt caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc
    30901 tgatcgataa catctgccgt aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc
    30961 gccgccaacg cacgctctat ttcgcctaac gagacggaat ccactttcac gccctgctca
    31021 cgcattaagc gcaaaatatg aatattggaa caggctttct gtgcaaagcg caccacatca
    31081 aactgtttca gcgctgcaat ctgccgacga ataatttgcg catcgtagac ccacaccggg
    31141 cagccaaatt cagcgggcaa acgcagcaga ttttcggcgg tgagatcggt atcggtgctg
    31201 aacagtgaat gtggcataac aaactccaga taagtgcttt tttatgatta cgccacatca
    31261 taaaaagaat aaaaaatatc gatttatgtc gagtctatgc aaaaatgata tggattaccg
    31321 gattgcgaga gagcgctaat ggccgccgtt aacttacgtc atattgaaat ttttcatgcg
    31381 gtaatgaccg ccggaagcct gactgaggcg gcacacctgc tacacacctc acagccaacc
    31441 gtcagccgcg aactggcgcg ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta
    31501 cgtgggcgat tacatcctac cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc
    31561 tggtacggac tggatcgaat tgtcagcgct gcagaaagtc tgcgcgagtt tcgccaggga
    31621 gaactgtcta ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa
    31681 ccctttctgg cacgttatcc cgatgtcagc ttaaatatcg tgccccagga atcaccgcta
    31741 cttgaagagt ggctctcggc ccagcgtcat gatttaggac tcactgagac actccatacg
    31801 cctgcgggaa cagaacgtac cgaattactc tctttagatg aagtgtgtgt gttacctccg
    31861 ggtcatccgc tggcggtaaa aaaggtatta acgccggatg attttcaggg tgagaactac
    31921 atcagccttt cccgtactga cagctatcgc cagttgctgg atcagctatt tactgaacat
    31981 caggttaaac gacgcatgat cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta
    32041 cgggcggggg taggtgtttc ggtggttaac ccgctcaccg cactagatta tgcggcaagc
    32101 ggtttagtgg tgcggcggtt cagtattgcg gttccgttca ccgtcagcct gatccgcccc
    32161 ctgcaccgcc cgtcatcagc gctggttcag gcgtttagtg ggcatttaca agcggggtta
    32221 ccgaaactgg tcacttctct tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga
    32281 cagcatcctc ggcatggatc gccgcggtat caaacacagg caaaacactg cgctcttctg
    32341 gcaccagtaa accaatttct gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa
    32401 ggcgagcaat cacttgcgca taataagcgc gtgacgcttc ggtaaattgc cccagacaca
    32461 gttcttcaaa aataatctga ttaattttcg cccgttcatc cgcttcagga ataagacaat
    32521 tgatggaaaa ttgttccgtc agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg
    32581 tacccagcag cgccacacga gtcattccgg ccccggtaat tgcacgtccg gtggcatccg
    32641 caatgtgtaa gaaaggcaga gtgcaacgtg actcaatggc atccgccact ttatgcatcg
    32701 tattggtaca taacacaata ccttctgcgc ccgcccgctg taagccaagc gccgcctcag
    32761 ccagaatgtc cccggtttta tcccattccc cgcgacgctg gcactcttct atttcatgaa
    32821 aatcgacgct atgtagcagc acttgcgcag agtgaagccc accaagccgc tgtttaatgc
    32881 cttcatttat caaacgatag taaggaatgg tggattccca gctcattcct cccagcaaac
    32941 caattgtttt cattcctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
    33001 agacaaacgc ctcaacggcc aagtgcccaa tctctattaa cgaaaaaagg gccggatgta
    33061 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
    33121 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtaat
    33181 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag cgccaatgct
    33241 atcaagcagt gtcaggaagg tcgcgccgat aatcatattc gacacccagt ttgtcgtcgt
    33301 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
    33361 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
    33421 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
    33481 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgacctgc
    33541 tttatctacc gtaaagaccg caatgaaggt cgcgaacata aaggtcagtc ccacgaccag
    33601 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
    33661 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
    33721 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
    33781 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    33841 catacgcaat acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    33901 atttggcagg aagaccacca gaataatcag cagaactgct ggtaaagcaa gaacccccaa
    33961 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    34021 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    34081 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    34141 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    34201 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    34261 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    34321 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    34381 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    34441 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    34501 tttttcctgc cagcagagaa taagacatag tgaaaaaata cgtgaacaat tcacacaggt
    34561 gtcaggtcgg aaatagcata aatatggatt aaattgctac ggcatgccgt tatgtgatgg
    34621 atattccaat tttaaaatta agttgaatta ttgagat
//
LOCUS       DAOFQD010000043        34383 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.043, whole genome shotgun sequence.
ACCESSION   DAOFQD010000043 DAOFQD010000000
VERSION     DAOFQD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.043"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(189..265)
                     /locus_tag="QBF46_002086"
     tRNA            complement(189..265)
                     /locus_tag="QBF46_002086"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Arg,seq:acg)
     gene            complement(269..361)
                     /locus_tag="QBF46_002087"
     tRNA            complement(269..361)
                     /locus_tag="QBF46_002087"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Ser,seq:gct)
     gene            complement(677..862)
                     /gene="csrA"
                     /locus_tag="QBF46_002088"
     CDS             complement(677..862)
                     /gene="csrA"
                     /locus_tag="QBF46_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDQ6674268.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1097..3727)
                     /gene="alaS"
                     /locus_tag="QBF46_002089"
     CDS             complement(1097..3727)
                     /gene="alaS"
                     /locus_tag="QBF46_002089"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDQ6674269.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQTDVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3855..4355)
                     /gene="recX"
                     /locus_tag="QBF46_002090"
     CDS             complement(3855..4355)
                     /gene="recX"
                     /locus_tag="QBF46_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDQ6674270.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4598..5659)
                     /gene="recA"
                     /locus_tag="QBF46_002091"
     CDS             complement(4598..5659)
                     /gene="recA"
                     /locus_tag="QBF46_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDQ6674271.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5739..6236)
                     /gene="pncC"
                     /locus_tag="QBF46_002092"
     CDS             complement(5739..6236)
                     /gene="pncC"
                     /locus_tag="QBF46_002092"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDQ6674272.1"
                     /translation="MTDSELMQLSERVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6381..7466)
                     /gene="mltB"
                     /locus_tag="QBF46_002093"
     CDS             complement(6381..7466)
                     /gene="mltB"
                     /locus_tag="QBF46_002093"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDQ6674273.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLLNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7722..8285
                     /gene="srlA"
                     /locus_tag="QBF46_002094"
                     /gene_synonym="gutA"
     CDS             7722..8285
                     /gene="srlA"
                     /locus_tag="QBF46_002094"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDQ6674274.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8282..9241
                     /gene="srlE"
                     /locus_tag="QBF46_002095"
                     /gene_synonym="gutE"
     CDS             8282..9241
                     /gene="srlE"
                     /locus_tag="QBF46_002095"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDQ6674275.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9252..9623
                     /gene="srlB"
                     /locus_tag="QBF46_002096"
                     /gene_synonym="gutB"
     CDS             9252..9623
                     /gene="srlB"
                     /locus_tag="QBF46_002096"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDQ6674276.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9627..10406
                     /gene="srlD"
                     /locus_tag="QBF46_002097"
     CDS             9627..10406
                     /gene="srlD"
                     /locus_tag="QBF46_002097"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDQ6674277.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10512..10871
                     /gene="gutM"
                     /locus_tag="QBF46_002098"
     CDS             10512..10871
                     /gene="gutM"
                     /locus_tag="QBF46_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDQ6674278.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10938..11711
                     /gene="srlR"
                     /locus_tag="QBF46_002099"
                     /gene_synonym="gutR"
     CDS             10938..11711
                     /gene="srlR"
                     /locus_tag="QBF46_002099"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDQ6674279.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11704..12669
                     /gene="gutQ"
                     /locus_tag="QBF46_002100"
     CDS             11704..12669
                     /gene="gutQ"
                     /locus_tag="QBF46_002100"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDQ6674280.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12666..14180)
                     /gene="norR"
                     /locus_tag="QBF46_002101"
     CDS             complement(12666..14180)
                     /gene="norR"
                     /locus_tag="QBF46_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDQ6674281.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFNVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14367..15806
                     /gene="norV"
                     /locus_tag="QBF46_002102"
     CDS             14367..15806
                     /gene="norV"
                     /locus_tag="QBF46_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDQ6674282.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALKLCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            15803..16936
                     /gene="norW"
                     /locus_tag="QBF46_002103"
     CDS             15803..16936
                     /gene="norW"
                     /locus_tag="QBF46_002103"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDQ6674283.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGIQA
                     TLDRQRNIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17065..19317)
                     /gene="hypF"
                     /locus_tag="QBF46_002104"
     CDS             complement(17065..19317)
                     /gene="hypF"
                     /locus_tag="QBF46_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDQ6674284.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLLVADGLPDAVRQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGAMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGELQNG"
     gene            complement(19470..19997)
                     /gene="hydN"
                     /locus_tag="QBF46_002105"
     CDS             complement(19470..19997)
                     /gene="hydN"
                     /locus_tag="QBF46_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDQ6674285.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20146..21156)
                     /gene="ascG"
                     /locus_tag="QBF46_002106"
     CDS             complement(20146..21156)
                     /gene="ascG"
                     /locus_tag="QBF46_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDQ6674286.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21416..22873
                     /gene="ascF"
                     /locus_tag="QBF46_002107"
     CDS             21416..22873
                     /gene="ascF"
                     /locus_tag="QBF46_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDQ6674287.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQTFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKYQSVQPTVAKEVSLN"
     gene            22882..24306
                     /gene="ascB"
                     /locus_tag="QBF46_002108"
     CDS             22882..24306
                     /gene="ascB"
                     /locus_tag="QBF46_002108"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDQ6674288.1"
                     /translation="MSVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24420..24839)
                     /locus_tag="QBF46_002109"
     CDS             complement(24420..24839)
                     /locus_tag="QBF46_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6674289.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24836..25147)
                     /locus_tag="QBF46_002110"
     CDS             complement(24836..25147)
                     /locus_tag="QBF46_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HDQ6674290.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25321..26082)
                     /locus_tag="QBF46_002111"
     CDS             complement(25321..26082)
                     /locus_tag="QBF46_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674291.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26107..26577)
                     /gene="hycI"
                     /locus_tag="QBF46_002112"
     CDS             complement(26107..26577)
                     /gene="hycI"
                     /locus_tag="QBF46_002112"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDQ6674292.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26570..26980)
                     /gene="hycH"
                     /locus_tag="QBF46_002113"
     CDS             complement(26570..26980)
                     /gene="hycH"
                     /locus_tag="QBF46_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDQ6674293.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26977..27744)
                     /gene="hycG"
                     /locus_tag="QBF46_002114"
     CDS             complement(26977..27744)
                     /gene="hycG"
                     /locus_tag="QBF46_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDQ6674294.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27744..28286)
                     /gene="hycF"
                     /locus_tag="QBF46_002115"
     CDS             complement(27744..28286)
                     /gene="hycF"
                     /locus_tag="QBF46_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDQ6674295.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28296..30005)
                     /gene="hycE"
                     /locus_tag="QBF46_002116"
     CDS             complement(28296..30005)
                     /gene="hycE"
                     /locus_tag="QBF46_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDQ6674296.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGVRKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30023..30946)
                     /gene="hycD"
                     /locus_tag="QBF46_002117"
     CDS             complement(30023..30946)
                     /gene="hycD"
                     /locus_tag="QBF46_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDQ6674297.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30949..32775)
                     /gene="hycC"
                     /locus_tag="QBF46_002118"
     CDS             complement(30949..32775)
                     /gene="hycC"
                     /locus_tag="QBF46_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDQ6674298.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32772..33383)
                     /gene="hycB"
                     /locus_tag="QBF46_002119"
     CDS             complement(32772..33383)
                     /gene="hycB"
                     /locus_tag="QBF46_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDQ6674299.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33508..33969)
                     /gene="hycA"
                     /locus_tag="QBF46_002120"
     CDS             complement(33508..33969)
                     /gene="hycA"
                     /locus_tag="QBF46_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDQ6674300.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34181..>34383
                     /gene="hypA"
                     /locus_tag="QBF46_002121"
     CDS             34181..>34383
                     /gene="hypA"
                     /locus_tag="QBF46_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease nickel incorporation protein
                     HypA"
                     /protein_id="HDQ6674301.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQ"
BASE COUNT         8117 a         9534 c         8848 g         7884 t
ORIGIN      
        1 ttactttact gcagattttt tgataccgct actaaagccg tatcaagtaa gagatggtgc
       61 atccgggagg attactcgcc ttgcggctcg cccttcgggc cgttgctaaa gcaacgttat
      121 cctccctggt gcctgcggtt attaccgcca cctcttccat accttcatcg ctgaaagtac
      181 gtagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta
      241 ctctatccag ctgagctacg gatgcaaatg gcggtgaggc ggggattcga accccggatg
      301 cagcttttga ccgcatactc ccttagcagg ggagcgcctt cagcctctcg gccacctcac
      361 cacacgcctc ttacgagtgc ttcgaagaac ttgtttatcg ctcatcgtcg ctgcgtggcg
      421 cacatattac tttctgagac ttataagtca aacaattttt cctacacttt tatcgtttgc
      481 acacttcacg ttcaattagt ctgtaaaaac gtcaaaaaga gtgttttatc aacagaagaa
      541 tggaggtctg acagatagta gtaatgcaaa aaaatggaga cttaagttga atgaacggga
      601 gtaaagcgaa aagactatag agtgaaggag aaattttgag ggtgcgtctc accgataaag
      661 atgagacgcg gaaagattag taactggact gctgggattt ttcagcctgg atacgctggt
      721 agatctcttc acggtgaaca gaaacttcct tcggggcatt tacgccaata cgtacctggt
      781 tgccctttac ccctaaaact gtcacggtga cctcatcccc aatcatgagg gtctcaccaa
      841 ctcgacgagt cagaatcagc attctttgct ccttgaaaga ttaaaagagt cgggtctctc
      901 tgtatcccgg cattatccat catataacgc caaaaagtaa gcaatgacaa acacattaca
      961 tctaagcagt catggcatta cattctgtta aacctaagtt tagccgatat acacaacttc
     1021 aacctgactt tatcgttgtc gatagcgtta atgcgaatgc cgtgaagcga gtccacggca
     1081 ttgcctgacg cttatattat tgcaatttcg cgctgaccca gcctttcaca ctggctaacg
     1141 ctgcaggtaa ggccgcagca tccgtaccac cggcttgcgc catgtcagga cgtccaccac
     1201 ccttgccgcc cacctgctga gcgaccatac caatcagttc ccctgctttc acacgatctg
     1261 tgacgtcctt agatacgcct gcaatcagag aaaccttacc ttcggctacc gttgccagca
     1321 cgataattgt cgaccccagc tgatttttta aatcgtcaac catggtacgc aacattttcg
     1381 gctcaacacc gctaagctcg ctaaccaaca gcttaacacc attaacatca attgccttac
     1441 tggaaagatt tgcgctctcc tgtgcggcag cttgttcttt aagctgttgt aactcttttt
     1501 ccagctgacg cgtacgttcc agtactgagc gcactttatc agccagatta ttgctatcgc
     1561 ctttcagcag atgtgcgact tcgcttaagc gatcgctgtc tgcatgaacg gtggcgatag
     1621 cgccttctcc ggttaccgct tcgatacgac gaacgcctgc agcagtaccc gattcagaga
     1681 tgatgcggaa cagaccaata tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg
     1741 tggagaaatc gcccatgctc agcacgcgta cgcgctcatc atacttctcg ccgaacagcg
     1801 ccatcgcacc tttcgctttc gccgcttcga gatccatgat gttggtttcg atcggcaaat
     1861 tgcgacgaat ctgtgcgttc accaggtctt cgaccgcacg aatctcttct ggtttcatcg
     1921 cttcgttgtg tgagaagtcg aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg
     1981 atacatgagt acccagaacc tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat
     2041 tcagacgaat acgggcacga cgagcctcat caacatcagt ctgcaccgcg tcgcccactt
     2101 tcagagaacc cgcagcaagt ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat
     2161 cttccaccgc aaaggagaag ttagcgcctt tcagttcgcc tttatcgcca acctgaccgc
     2221 cggattccgc atagaatggc gtttgatcca gcacgaccac agcttcctgg cctgcattga
     2281 tggcatcaac cgctttacca tcaacaaaca gcgcggtcac tttgccgttc agttccagat
     2341 ggtcatagcc tttaaattca gatgcactat caacacggat cattgcgttg taatcggcac
     2401 caaagccgct ggcttcgcgc gcgcgacgac gctgctcttc cattgctgct tcaaaaccag
     2461 cttcgtcaac tttgatgttg cgctcacgac aaacatcagc cgtcaggtca accgggaagc
     2521 cataggtgtc gtacagacgg aaagccgttt caccatccag cgtatcacca gaaagttttg
     2581 ccagctcttc atccagcaac gccagaccgc gctccagagt acgggcaaac tgctcttctt
     2641 cagtcttcag cacctgctca acctgcgcct gctggcgttt caggtcttca cccgcagagc
     2701 ccataacgtc gatcagcgga ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca
     2761 tattgccgtg acgcactgcg cgacgaatga tacgacgcag tacataacca cggttttcat
     2821 tggacggcat tacgccatcc gcgatcagga acgcacaaga acgaatgtgg tcagcgatta
     2881 cgcgcagcga tttattgctc agatcggtcg cgccagtgac tttcgctacc gcctggatca
     2941 acgtgcggaa caggtcgatg tcatagttag agttaacgtg ttgcagcacc gcagcaatac
     3001 gctccagacc cataccggta tctacagacg gcttcggcag cggttccatc gtgccatctg
     3061 cctggcggtt gaactgcatg aagacgatgt tccagatctc aatgtagcgg tcgccgtctt
     3121 cttccggact tcccggaggg ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg
     3181 tgcacgggcc gcacggacca gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg
     3241 cacctttgtt atcgccgatg cgaataatac gttcgcgcgg gatcccgact tctttttccc
     3301 agatttcgta ggcttcgtcg tcgctttcat agacggtaac ccacagacgc tctttcggca
     3361 gggcaaacca tttttcgctg gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga
     3421 aatagtcgcc gaagctgaag ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt
     3481 aaccgacgtt ttccaggtcg ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg
     3541 tagcgcggga ataattacgc ttgtcgagcc caaggaacac atccttgaac tggttcatcc
     3601 cggcgttggt aaacaacaaa gttgggtcgt tatggggtac cagggagctg ctggcaacta
     3661 cctgatgtcc cttactatgg aaaaagtcga gaaacgcctg acggatctca gcggtgctct
     3721 tgctcataat tatcctgaaa tcaagctaac gaaatatcgc caccagctcc agcgtgtctt
     3781 aaccgccggg ctggtaactg aaaagtggga ataagataag ttttcttgac tgggaagtaa
     3841 aataccgtat gcgttcagtc ggcaaaattt cgccaaatat cctggatatc ttccatcaga
     3901 tagccacgat agagcagaaa acgctggatc ttaacttttt ctgaaaagac agttggcaaa
     3961 ggttcaccat attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat
     4021 tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt tctgattcag ttcctgacga
     4081 atacgtgcag gtccataacc tttgcggcta cggctggcga taaagcgcgc aacaaatcgg
     4141 ctgtcatcga gatagccatg ttcatggcac caggcaataa cgcgttcgta atcttctgcc
     4201 gtagcatcaa tctcttccgg gccatttttg cccataatcg gtgccgcgag tttacgtcgc
     4261 agttcttgct cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga
     4321 gcatatgccg ggcgacggga cgttgattct gtcatggcat atccttacaa cttaaaaaag
     4381 caaaagggcc gcagatgcga cccaggtttt gctgataaac agatttaata gtcaatgagt
     4441 gtgcagaatg ttcccgcagg cccaattcgt tagaggatcg aggtgagagt aatttttcac
     4501 gccagtgctg gcctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc
     4561 cgtgcacaca ttatctatta tcatcaataa cgaacgatta aaaatcttcg ttagtttctg
     4621 ctacgccttc gctgtcatct acagagaaat ccggcgttga gttcgggttg ctcagcagca
     4681 actcacgtac tttcttctcg atctctttcg cggtttccgg gttatctttc agccaggcag
     4741 ttgcattcgc tttaccttga ccgatcttct cacctttgta gctgtaccac gcgcctgctt
     4801 tctcgatcag cttctctttt acgcccaggt caaccagttc gccgtagaag ttgatacctt
     4861 cgccgtagag gatctggaat tcagcctgtt taaacggcgc agcgattttg ttcttcacca
     4921 ctttcacgcg ggtttcgcta cccaccacgt tttcgccctc tttcaccgcg ccgatacgac
     4981 ggatgtcgag acgaacagag gcgtagaatt tcagcgcgtt accaccggta gtggtttccg
     5041 ggttaccgaa cattacacca attttcatac ggatctggtt gatgaagatc agcagcgtgt
     5101 tggactgctt caggttaccc gccagcttac gcatcgcctg gctcatcata cgtgccgcaa
     5161 ggcccatgtg agagtcgccg atttcgcctt cgatttccgc tttcggcgtc agtgccgcca
     5221 cggagtcaac gacgataacg tctactgcgc cagaacgcgc cagggcgtca cagatttcca
     5281 gtgcctgctc gccggtgtcc ggctgggagc acagcaggtt atcgatatcg acgcccagtt
     5341 tacgtgcgta gattgggtcc agcgcgtgtt cagcatcgat aaacgcacag gttttacctt
     5401 cacgctgcgc tgcggcgatc acctgcagcg tcagcgtggt tttaccggaa gattccggtc
     5461 cgtagatttc gacgatacgg cccatcggca gaccacctgc cccaagcgcg atatccagtg
     5521 aaagcgaacc ggtagagatg gtttccacat ccatggaacg gtcttcaccc aggcgcatga
     5581 tggagccttt accaaattgt ttctcaatct ggcccagtgc tgccgccaac gctttctgtt
     5641 tgttttcgtc gatagccatt tttactcctg tcatgccggg taataccgga tagtcaatat
     5701 gttctgttga agcaattata ctgtatgctc atacagtatc aagtgttttg tagaaattgt
     5761 tgccacaagg tctgcaatgc atacgcagta gcctgacgac gcaccgcatc acggtcaccg
     5821 ctgaagcatt cccgccgggt aatgccttca ccgcgggcag tggcaaaagc aaaccagaca
     5881 gtgccgacag gcttctcttc actgccgcca tccggcccgg cgataccact aatagacacg
     5941 gcgtaatcag cacgagccgc tttgagtgcg cctatcgcca tttccaccac gacgggttcg
     6001 ctcaccgcgc catgctgcgc cagcgtctct tcgcgtacgc cgatcatctg cgctttggct
     6061 tcgttactgt aggtgacaaa tccgcgttca aaccaggcgg aactgccggc aatatcggta
     6121 atcactttcg ctacccaacc gccggtacaa gactcagcag ttgttacggt tgcgccacgg
     6181 gctttcagcg cctgcccaac ccgttcgctt aactgcatca gttcactgtc agtcatcaca
     6241 acctctgtca gtcaaaaatt tatgccggac aagatagcac tttcgtcagg aagatggtgt
     6301 tggcgttgca attttacgag gaggattcag aaaaaagctg attagccaga gggaagctca
     6361 cgcccccctc ttgcaaatag ttactgcacg cgcgccagcg ccacggcttg tcctaactgc
     6421 cagaccgcca ttgcgtaatg ggtgctgtgg ttataacggg taatggtgta gaagttcggc
     6481 agaccgtacc agtactggta accggtaccg acatccagac gcagcaggct ggcttgttga
     6541 tggttgccca gcggctgctg tggcgttaaa cctgccgcgg caagctgaga aatgctgtac
     6601 ctggttttga agccatttag caagcctgga gcctgaccgt ttgccattac cgcgacctga
     6661 tcgcctttca cccagccgtg cgctttgaaa taattcgcca cgctaccgat cgcatcaacc
     6721 ggatcccaca ggttgatatg cccgtcgccg ctgaaatcta ccgcatattg tttgtaagac
     6781 gacggcataa actgtccgta gcccatcgcc ccggcaaagg aacctttcag attgagcgga
     6841 tcgtcctgct cgtcgcgcgc catcagcagg aaggtttcca gctcgccaga gaaatactcc
     6901 gcgcggcgtg ggtagttaaa tgacagcgtt gccagcgcat cgaggatgcg agttttcccc
     6961 atcacgcgcc cccagcgggt ttcaacgccg ataatcccga cgataatttc cggcggtacg
     7021 ccatacacct gccacgcgcg attcaacgca tcttcatact gattccagaa aaccacaccg
     7081 ttctgcacgt tgtccggcgt aataaatttt ttgcgataac gcagccatgc gccgttcgga
     7141 cctgatggcg gtttcaccga tgtggttggt gcctggttat ccatcagccg cagtaccgaa
     7201 tccagacgtt tcgcctggga gagaatttcc tgcaactgct gacgatcgaa accgtgtttg
     7261 ttcaccattt tgtcgatgaa ctgctgggca ttcgggttat tagcgaaatc gccgcccatc
     7321 tgcatcacat tgtgctgcgg ctcaagcagg aagccgccag acggcgttcc ggtggtcgta
     7381 tcagtctcag taggttttgg cttgctgcta caggcggcaa gcaacacaaa aaggggaagc
     7441 aatgttacat aacgacgctt gaacatgagg ggtccattta acagattcaa ccaggggcaa
     7501 gtatggtaaa gcatcacgcc ccgcacaagg aagcggtagt cactgcccga tacggacttt
     7561 acataactca actcattccc ctcgctatcc ttttattcaa actttcaaat taaaatattt
     7621 acctttcatt ttgcgatcaa aataacactt ttaaatcttt caatctgatt agattaggtt
     7681 gccgtttggt aataaaacaa taaatcctga aggagagaac aatgatagaa accattactc
     7741 atggtgcaga gtggtttatc gggctgttcc aaaagggcgg agaggtgttt accgggatgg
     7801 tgaccggcat tcttccgctg ttgattagcc tgctggttat catgaacgca ctgattaatt
     7861 ttatcggtca gcatcgtatt gaacgttttg ctcaacgttg cgccggtaac cctgtttccc
     7921 gttacctact gttaccgtgc attggcacgt ttgtcttttg caatccgatg accctaagcc
     7981 tgggtcgctt tatgccggaa aagtacaaac ccagctacta cgcggcggcc tcttatagct
     8041 gccactcaat gaatggcctc ttcccccata tcaaccctgg cgaactgttt gtttatcttg
     8101 gcattgccag cggtctgaca acgctgaacc tgccacttgg cccactggcg gtgagctatc
     8161 tgctggttgg tctggtcacc aacttctttc gcggctgggt gaccgatctg accaccgcca
     8221 tttttgagaa aaagatgggc attcaacttg aacaaaaagt tcacctggca ggagcaacat
     8281 catgacgcgt attcggatcg aaaaaggaac gggtggctgg ggcggcccgc ttgagctgga
     8341 agccacgccg ggcaaaaaaa tcgtctatat caccgccggt acccggcctg cgattgttga
     8401 caaactggca caacttactg gctggcaggc tattgacgga tttaaagaag gtgaacccgc
     8461 ggaggcggaa attggtgtcg cggtaatcga ctgtggcggc acattacgct gcggcatcta
     8521 tccgaaacga cgtattccca ccattaatat ccactcgacg ggcaagtccg gtccgctggc
     8581 gcagtacatt gtggaagata tttatgtctc tggcgtaaaa gaagaaaaca tcactgtagt
     8641 gggtgatgcg acaccacaac cctcttccgt gggccgtgac tatgacacca gtaagaaaat
     8701 caccgaacaa agcgatggtt tactggcgaa ggtaggaatg ggcatggggt ccgccgttgc
     8761 ggtgctgttt caatctggtc gtgacaccat cgacactgta ttaaaaacca ttctgccgtt
     8821 tatggcattc gtctcggcgc tcattggcat cattatggct tctggccttg gtgactggat
     8881 tgcccacggt cttgctccgc tggcgagcca tccactgggt ctggtcatgc tggcgctcat
     8941 ctgctccttc ccactgcttt cacctttcct cggcccaggc gcagttatcg cacaggttat
     9001 cggcgtattg attggcgtgc agattggtct cggcaatatt ccgccgcatc tggctttacc
     9061 ggcactgttt gccatcaacg cgcaggcggc ctgcgacttc atcccggtcg gtttgtcgct
     9121 ggcggaagcc cgtcaggaca cggttcgcgt cggtgtccct tctgtactgg tgagccgctt
     9181 tttaaccggc gcaccgactg tactgatcgc ctggtttgtc tccggtttta tctatcaata
     9241 gaggctgaaa catgaccgtt atttatcaga ccaccatcac ccgtatcggc gcgagtgcca
     9301 ctgacgccct cagcgaccag atgctcatca cctttcgtga aggcgcgcca gcggacctcg
     9361 aagagtattg cttcattcat tgccacggcg agttgaaagg tgcactccat cccggtttgc
     9421 aatttacgct cgggcagcat cgctatccgg tgaccgctgt tggcagcgtg gcggaagaca
     9481 accttcgcga actgggtcat gtcaccctgc gcttcgatgg tttaaacgaa gcggaatttc
     9541 cgggcactgt ccatgtggca ggccctgtac ccgacgatat cgcgccggga tcggttttga
     9601 agtttgaatc tgttaaggag taaaaaatga atcaggttgc cgttgtcatc ggtggtgggc
     9661 aaaccttagg cgcgttcctg tgccacggtc tggctgccga ggggtatcgc gtcgcggttg
     9721 tcgatattca gagcgacaaa gccgcaaatg tggcacaaga aattaacgcc gaatatggtg
     9781 aaggaacagc gtatggtttt ggtgctgacg ccaccagcga gcaaagcgtt ctggcgctct
     9841 ctcgtggggt agatgaaatc tttggtcgcg tggatttgtt ggtctacagc gccggaattg
     9901 caaaagcagc ctttatcagc gacttccagc tcggcgattt tgaccgttcg ctacaggtga
     9961 atctggtggg ttatttcctg tgtgcgcgtg aattttcgcg tttgatgatc cgcgacggga
    10021 ttcaggggcg cattattcag atcaactcga aatccggcaa agtgggcagc aaacacaact
    10081 ctggctacag cgcagcgaaa tttggtggcg tcgggctgac tcaatcactg gcgctggatc
    10141 tggcggagta cggtattacg gttcattcac tgatgctcgg taacctgctg aaatcgccta
    10201 tgttccagtc actgttgccc caatacgcga ccaagctggg tatcaaaccg gatcaagtcg
    10261 agcagtatta catcgataaa gtaccgctca aacgcggctg cgattatcag gatgtgctga
    10321 atatgctgct gttctacgcc agtccaaagg catcgtactg cactggtcag tcgatcaatg
    10381 tcaccggcgg tcaggtgatg ttctgatcaa cagcggagat ccgttaagga tctccgtgag
    10441 actaatagaa tgcctgatgc gctacgctta tcaggcctac aggacttccg ccactacatt
    10501 aaggaaaagt tatggtatcc gcactcatca ccgtcgccgt tatcgcctgg tgtgcgcaac
    10561 tggccttagg cggctggcaa atttctcgtt ttaaccgtgc cttcgacaca ctatgccagc
    10621 aagggcgggt tggcgtgggc cgttcaagcg ggcgctttaa accgcgggtc gtggtcgcca
    10681 tcgccctgga cgatcagcag cgcatcgccg acaccttgtt tatgaaagga ctgaccgtct
    10741 tcgcacgacc gcaaaaaatt cccgcaatta ccggcatgca tgcgggtgat ttacagcccg
    10801 atgtgatctt tccccatgat ccactatcac agaatgctct atcattggcg cttaaactga
    10861 aacgtggata atttcgttgt gaatgttact tgcttgcgaa gttatcattt tgaaacctaa
    10921 atcaggtaat cacgcccatg aaacctcgtc agcgtcaggc cgccattctg gagtatctgc
    10981 aaaagcaggg taaatgctcg gttgaagaat tggcgcaata ctttgacacc acaggcacaa
    11041 ccattcgcaa agatctggtc attctggaac atgccggaac cgtcattcgt acttatggcg
    11101 gagtggtgtt gaataaagag gaatccgatc cgcctatcga tcataaaaca ctcatcaaca
    11161 cccacaagaa agagctgatt gcagaagctg ccgttagttt tatccatgat ggcgattcga
    11221 tcattcttga tgctggcagt accgttttgc agatggttcc cctgctctcg cgctttaata
    11281 acatcacggt gatgaccaac agcctgcata tcgtcaatgc gctatccgaa ctggataacg
    11341 aacaaactat cctgatgcca ggcggaacgt ttcgcaaaaa atcggcctca tttcacgggc
    11401 aactggcaga gaatgccttc gagcatttca ccttcgataa attgtttatg ggcaccgacg
    11461 gcatcgatct caatgcgggc gtaaccacct ttaacgaggt ttataccgtc agtaaggcaa
    11521 tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc atcaaagttt ggccgtaaaa
    11581 gccccaacgt agtttgcagt cttgaaagcg tcgataagct gattaccgac gcaggtatcg
    11641 atccggcgtt tcgtcaggcg ctggaagaga aagggatcga tgtgatcata accggagaga
    11701 gcaatgagtg aagcactact gaacgcggga cgtcagacgt taatgctgga gttgcaggaa
    11761 gcaagccgtt taccggaacg tctgggcgat gattttgttc gcgccgccaa tatcatcctg
    11821 cactgcgaag gcaaagtggt ggtttcggga attggcaaat cgggccacat tggtaagaaa
    11881 atcgccgcaa cacttgccag taccggcaca ccggcttttt ttgtccatcc ggcagaagca
    11941 ctgcacggcg atctggggat gattgaaagc cgcgatgtga tgctgtttat ctcttactcc
    12001 ggtggcgcga aggaactgga tctgattatt ccgcgtctgg aagataaatc tatcgcgctg
    12061 ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg cggcaaaagc ggtgctggat
    12121 atctccgtag aacgcgaagc ctgcccgatg caccttgcgc cgacctccag caccgtcaat
    12181 accctgatga tgggtgacgc gctggcgatg gcggtcatgc aggcgcgcgg atttaatgaa
    12241 gaagattttg cccgctccca cccagccggg gcactgggcg ctcgcttgct gaataaagtg
    12301 catcatctga tgcgccgtga cgatgccatc ccacaggtgg cgttaaccgc cagcgtgatg
    12361 gatgcgatgc tggaactcag ccgcaccggt ctggggctgg tggcggtatg tgacgctcaa
    12421 caacaggtac aaggcgtctt taccgacggc gatttacgtc gctggctggt aggcggcggg
    12481 gcactcacca cgccagttaa tgaagcgatg acgacaggcg gcaccacgtt acaggcgcaa
    12541 agtcgcgcca tcgacgccaa agagatcctg atgaagcgca aaatcactgc cgcaccggtg
    12601 gtggatgaaa acggcaaact caccggcgca attaacctgc aggatttcta tcaggccggg
    12661 attatttaat ccttcaatcc cagacgtttc gccagccgat gcaggttggc gacgtcggtt
    12721 tccagcatcc gcgcgcaggc agcccagttg tgatgatttt gtgccagtgc ctggcgaatg
    12781 gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt gcttaacaac gggcaccgcc
    12841 gccgcttctg gcggcggcaa cgtcacctca ggaaaagcaa aatgttgcgc ctcaagaatc
    12901 acttcatcgc cgttgcgggt ggctctcgcc agcactaccg cccgatgaat agcatgttcc
    12961 agttcgcgca cgttccccgg aaaacgatag tgttgcagta aatttcgcgc tccggcactt
    13021 aataccacgc gggagagccc cagccgcaaa cgacactgct cgcagaaata ccccgccagc
    13081 agaatgacat catcgccccg ctcacgcagc ggcggcaccg aaagtggaaa cacgctcagg
    13141 cgatgaaaca agtcagcgcg aaatcgccct gccagcacct cttcgcgtaa gtcgcggtta
    13201 gtcgccgcca gcacgcgcac atcgacccgc aaactacggt catcgccaac gcgctgaata
    13261 tcgccatact gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa ctcgccgatc
    13321 tcatccagaa acagagtgcc gttatccgcc atttcaaact tcccgctgcg gttactgata
    13381 gcgccagtaa acgctccttt cacatgcccg aacaactcac tttccgccac actttccggc
    13441 agtgcggcac agttgagata gaccagcgga ttcaccgccc gtggcgaggc ttcatgaatc
    13501 gctttcgcca ccagctcctt accggttccc gtctcaccgc tgatcaggac gttgagatcg
    13561 gacgccgcca caatctcaat ctcttttttc agttgcgtca tgccagggga caagccaatc
    13621 atctgcgtct gtttcaccgc ttcaaacggc gtggcatcgc ctggcatcat attctggctt
    13681 tccagttgtt caatcagcaa cgcattgctt aacgctcccg ccgccagcgc ggcaatcagc
    13741 cgtaactctt cgtcgctgaa aacattgaac tgatcgggct gcatcccgtc gagcgtcagt
    13801 gcgccgatca ggttttgtcc ggcaaacaat ggcagaccaa cgcaggcgtg aaccttcaga
    13861 ctctcctgcc cgggaatcaa accgtcatag gggtcgggca attcgctgtc tgcgggaaag
    13921 cgcaccacat ccccggcgcg ggcaatcgct tccagccgtg gatgcccttc cagcgcaaag
    13981 cgtctaccga gtacatcctt tgccagaccg tcgatggcaa gcggaataaa ctgccgcgaa
    14041 tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct gacgtagcgt ggtgatcagg
    14101 cgctgaaaac gatcctgatg accaatccca cgctgcaatt cgatggcgat attcgccagc
    14161 acatcaacgg aaaaactcat ctttgcctca ctgtcaattt gactatagat attgtcatat
    14221 cgaccatttg attgatagtc attttgacta ctcattaatg agcataattt tatttataga
    14281 gtaaaaacaa tcagataaaa aactggcacg taatctgcaa ttagcaagac atctttttag
    14341 aacacgctga ataaattgag gttgctatgt ctattgtggt gaaaaataac attcattggg
    14401 ttggtcaacg tgactgggaa gtgcgtgatt ttcacggtac ggaatataaa acgctgcgcg
    14461 gcagcagtta caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc gacaccgtcg
    14521 accataaatt cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat ctggcggata
    14581 tcgattacat cgtgattaac catgcagaag aggaccacgc cggggcgctg accgaactga
    14641 tggcacaaat tcccgatacg ccgatctact gtacagccaa cgctatcgac tcgataaatg
    14701 gtcatcacca tcatccggag tggaatttta atgtggtgaa aactggcgac acgctggata
    14761 tcggcaacgg caaacagctc atttttgtcg aaacaccaat gctgcactgg ccggacagca
    14821 tgatgactta cctgacaggc gacgcggtgc tgttcagtaa cgatgctttc ggtcagcact
    14881 actgcgacga gcatctgttc aacgatgaag tggatcagac ggagcttttc gagcagtgcc
    14941 agcgttacta cgccaatatc ctgacgccgt tcagccgcct ggtaacaccg aaaattaccg
    15001 agatcctggg ctttaactta ccagtcgata tgatagccac ttcccacggc gtggtatggc
    15061 gcgataaccc gacgcaaatt gtcgagctgt acttgaaatg ggcggctgat tatcaggaag
    15121 acagaatcac cattttctac gacaccatgt cgaataacac ccgcatgatg gctgacgcta
    15181 tcgcccaggg gattgcggaa accgacccac gcgtggcggt gaaaattttc aacgtcgccc
    15241 gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg ctggtcggca
    15301 cttcgacgat gaataacgtg atgatgccga aaatcgccgg gctggtggag gagatgactg
    15361 gtttacgctt ccgtaacaaa cgcgccagtg ctttcggctc tcacggctgg agcggcggtg
    15421 cggtggatcg tctttccacg cgcctgcagg atgcgggttt cgaaatgtcg cttagcctga
    15481 aagcgaaatg gcgaccagac caggacgctc tgaagttatg ccgtgaacac ggtcgcgaaa
    15541 tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg gtagttaaag
    15601 aagaaacctc tgccaccacg acggctgacc tcggcccacg gatgcagtgc agcgtctgcc
    15661 agtggattta cgatccggca aaaggcgagc caatgcagga cgttgcgcca ggaacgccgt
    15721 ggagtgaagt cccggataac ttcctctgcc cggaatgctc cctcggcaaa gacgtctttg
    15781 aagaactggc atcggaggca aaatgagtaa cggcattgtg atcatcggtt cgggcttcgc
    15841 cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc actattccat taaccctgat
    15901 tgccgccgac agcatggatg agtacaacaa acctgacctc agccatgtta tcagtcaggg
    15961 gcaacgtgcc gatgacctta cccgccagac ggcgggtgaa tttgccgagc agtttaatct
    16021 gcacctgttt ccacaaacct gggtgacgga tatcgatgcc gaagcccgtg tggtgaaaag
    16081 ccagaataat cagtggcaat acgacaagct agtactggca accggtgcca gtgcctttgt
    16141 cccgcctgtg cctgggcgtg agttaatgct gacgttaaat agtcagcaag agtatcgcgc
    16201 ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt gttggcggtg gtttgattgg
    16261 tagcgaactg gcgatggatt tttgtcgtgc aggcaaagcg gtcacgctaa tcgacaacgc
    16321 tgccagtatt ctggcgtcgt taatgccacc ggaagtaagc agccgcttgc agcatcggtt
    16381 gacggagatg ggcgttcatc tgctgttgaa atctcagtta caggggctgg aaaaaacgga
    16441 ttctggcatt caggcaacgc tggaccgcca gcgcaatatc gaagtggatg cggtaattgc
    16501 cgccaccgga ctgcgcccgg aaaccgccct ggcacgacgc gccgggctga cgattaatcg
    16561 cggcgtttgc gtcgatagtt atctgcaaac cagtaatacc gatatttacg cgctgggcga
    16621 ttgcgcggaa attaacggtc aggtattgcc gttcctccag ccgattcaac ttagcgcgat
    16681 ggtgctggca aaaaatcttc tcggcaataa cacgccgctg aaactcccgg cgatgctggt
    16741 gaaaatcaaa acgccggaat taccgctgca tctggcaggc gaaacccagc gtcaggattt
    16801 acgctggcaa attaataccg aacgccaggg aatggtggcg cgcggcgttg acgatgctga
    16861 ccagcttcgc gcctttgtgg tcagtgagga tcggatgaaa gaggcatttg gattgttgaa
    16921 aacattgccg atgtaggtgg gctactgtgc ctaaaatgtc ggatgcgacg ctggcgcgtc
    16981 ttatccgatc tacggggagc gcatatgtaa gccggataag gcatttacgc cgcatccggc
    17041 aaaggtgtcc aaatgcaaca cgctttatcc gttctggagt tcacccgcta accaacgcgc
    17101 cgcagcaata accccctgcc ccagagacaa accgccatca cccgccggta aactctgtgg
    17161 aaagagcaat gtgaaatcag cgagataatg cgccagacgt gcacgcagca aacggttatg
    17221 aataaccccg ccgctaaata ccagcgtagt gataccacgc atcgtggcct gctcacgcat
    17281 caacgcggca aaaccctgcg ccagcgcatc atgaaacgcc cacgcgcgtt gattaaccgg
    17341 tgcctgccag ttcagccact gctgccagaa agtggcgaga tccagttgat tgtccaccag
    17401 cggcattgtc accggatgcg tcactccgtg gcatgaggct gcgagcgcct ccagagcaca
    17461 agccgcttca ccttcataac ttaacgtggc tggcgcacag cccagtgccg ccgccactgc
    17521 atcgaaaaaa cgcccacacg atgacgccag cggcgcgtta attccacgct caatggcccg
    17581 cgccagcacg ctccagtttt gctgttgcac acttgctgtt tcagagtaat tctgccactc
    17641 cggcacaaag cgcaggcact gcgccagcag gtttcgccac ggctgcttcg ctgccaaatc
    17701 gccacccgga agcgccaccg caggcaagcc gcccaggtgc tggcattcgc gatagttcac
    17761 ccgcaggcac tcgccgcccc acaaagcgcc gttctccccc ataccgatac cgtcgagcgt
    17821 caaagcaatg acatcaccgc catccagcgg ccactgatgc tctgccagac acgccgctgc
    17881 gtgggcatga tgatgcagca ccgtttgcgt cggcagattc atttcgcgcg cccactggct
    17941 ggagacatag cccggatgcg cgtcatgcac aacgtattgc ggagtgaaat cgtagatgtt
    18001 ttgcatcagg cgtaacgctt cgcgccactg catctggatg ccatcgtcac ttaaatcgcc
    18061 cagatgctga ctcaacaccg cttgttcacc gcgcaccagg cagaaggtat ttttcagatc
    18121 cgcgccgaga cacagcacag gcggaacatt tttaaagccc ggaggcaaag ccagcgcatc
    18181 cggtacatac ccccgcgaac ggcgcagcat ttcgccgctt tcgcgcacca ccgaatcatc
    18241 catccgctgc acgatgtcgc ggttatgtat caagaatccg tcggcaatgc cctgcaaatc
    18301 cgccagcgcc tgttcgttgc tgatagccgg aggtttaccg ctcaggttgc cggaggtcat
    18361 caccagcggg cattgcagtt cctgtaacag caaatgctgg agcgggttcg caggcaacat
    18421 cgccccgact tcgttaaggc caggggcgat atcatcacaa agctcaggta cgtatttttt
    18481 atccaccagc acaatcggcg cggcgggggt ggtaagcaac tggcgcacag cgtctggtaa
    18541 accgtcagcc actagcaaca tgaccgccag cggtttcgcc gggcgatgtt tgcgcgcccg
    18601 aagtgtcgcc accgcgttac tgttacgtgc atcgcaggca agatgaaatc cgccaatccc
    18661 tttgatggcg acaatgttgc ccatttttaa ctgtgcgata gctgcctgta atgccgcctc
    18721 ttgttccgca tgttcaccat gacttaccca ttcaagatgc gggccacatt ccgggcaggc
    18781 caccggctgg gcgtggaagc gacgatcgag cgggtcgcgg tactctttat cacaggccgg
    18841 acacagcgga aacgccgcca tcacggtaaa cgggcggtcg taaggcatcg cgcgaataat
    18901 ggtgaaacgc gggccgcagt gggtacagtt gataaacgga taacgataac gccgctcgcc
    18961 tggggtattc atttcggcaa gacaggcagg gcaagtggca gcatccggga caatttgcgt
    19021 attcatggca ccgcccgcgc tttggcggat agtgaactcg gtgggcagtt gtgaccagat
    19081 aaacggctca cgctcgacgc tatcaatacg cgccagtggc ggacagtgtt gatgcaattg
    19141 aacaagaaac gtttccgggt cttccagcag ccggacttct acgccatcgc cgtcattaca
    19201 gacatcgccg tgaagattta attgctgtgc cagctgccag acaaacggac gaaaaccgac
    19261 gccctgcact ttgccacgaa tacgcagttg gacaccgcaa gatgtgtttt ttgccattga
    19321 gttattcccg ccatcatgaa ttgcgtaacc cgtccagccg gacacgacag cgtcgcatcc
    19381 ggcagtcaca ggtcggcgat accgcccgct ccgtattcta cgaatatttc cgggaattcc
    19441 tttgatgcca gaacagttct gtaagatttt tagaacatca gcgccgtacg gcggcgtttt
    19501 tctgcgctca gttgttcaag tttattacga tcgacacaaa tcagcgcatg agtcgggcaa
    19561 gccgccatac acgccgggcc gtcttcacga tggttgcaca ggtcgcattt attggcttcg
    19621 gctttgtcag cccgtacatt cagacccgcg ccgctgttgc gaatcaccgg acgtaccacc
    19681 acttccatcg cgccatacgg gcaagccaca acgcaggttt tgcaaccaat gcaacgttcc
    19741 tgcatcacat gaacaaaccc tttatcacgg ctgatagcac cattcgggca gacgttagcg
    19801 cacggtgcat cttcacactg acggcaaact gtcgccgtgg aaatgttcac acctttaatg
    19861 acatggatac gcggtaaaaa tgtttccggg gtcagcgatg cacagtcctg attttcctga
    19921 tgagaaacca cgcacgccac ttcacaggta cggcaaccaa tacatttact cgcgtcagca
    19981 atgatgaaac ggttcatcaa attctccagc aatgacagtt aatgcgccga tacattcaca
    20041 aatcatgcca gtttttaatt cactgttatt taaggaaatt aatttctgta atgcaggaaa
    20101 aaagatgtca tcgacactag tgacgatgac atgtgatgac aatgtttatc gcgaaagagc
    20161 aatgagtgag tcgcggcgga tcagttttcc gctgaaggtt ttcggcggtg agaaatcccc
    20221 gccatcgagc ataaaaatca gccgtccaat aatttcctga atcatctcag tcaccggaat
    20281 ttttacgctg gagagcgccg gaacgatata gggggcaatt gcgatatcat cgaatccgat
    20341 aactgacacc tgctctggca ccgctacgcc gcgctcgtgc aacgctttca tcgcacctat
    20401 cgccatatcg tcgttactgg caactaacgc gctaaattta gccccacgtt cgagcaacgt
    20461 ttctacccct tcggccccgc tggcgggcgt ccatttaccg ttagcgataa gtttttcatt
    20521 gagcgcaata ccatgctgcg ccagcgcgtc tttatacccg gcaagacgtt caatgctggt
    20581 gggggaatcc atcgagccgg taaggaaagc aatctcctga tgcccggcgt ttatcaactc
    20641 tgccacggcg ttaaagctgg tctgtttatg atcgcaccag acgctatggc tgctattttt
    20701 gcgcaggcgg cgattaagca ccattatcgg ctgactgtgc gcgtcaatga tgtcatcaat
    20761 ctcatccacg cttaaaaaac gcgggtaaat cataatggcg tcgcagcgca aatccagcag
    20821 atactgaatc gcctggcgct cttcttcggc gctgtgttta ccatctgcca atagcaactg
    20881 tcgccctttc tcttccgcca ttcgcgcggc atgaaacagt aattcactaa aataaatgcc
    20941 gtgataaagc gtgttggtca ctaccagccc cagcgtctga gtactcttcg ccgacagatt
    21001 gcgggccagc aaatttgggc ggtaaccgct ctcttctacc gcctgaaaca cgcgatcttt
    21061 agtctcctgg ctgacgtagc cattacctga aagcacgcgg gaaacggtcg cttttgaaac
    21121 cccggcgcgc ttcgccactt ccagcatcgt cgtcatcatt ttcatccctt tacacgcaat
    21181 caacgcagtg tactgcaccg tttgccgatt gtccttgcgc aatcggcggg aaaaatattc
    21241 aggtgaccgg tttcacaaat ataaaaaatg aacaattaac tctcttgctt atttagtgac
    21301 aactattcat gattttgtga aaccggtttc ctaattccgt ttcagcatcg gcatttttcc
    21361 gttacgtcga ctgataacaa ctacatctac cctactgata acaggataaa atccgatggc
    21421 caaaaattat gcggcgctgg cacgctcggt gatagcggca ctgggcggcg ttgataacat
    21481 ctcggcggtc acgcactgta tgacgcgttt gcgctttgtt atcaaagatg accagcttat
    21541 cgacagcccg acgttaaaaa ccatccccgg cgtgctcggc gtggtacgta gtgacaacca
    21601 gtgtcaggtg attatcggca ataccgtttc acaaaccttt caggaagtcg tcagcctgct
    21661 gccgggagat atgcagcccg cacagcccgt gggtaaaccc aaactcacgc tgcgtcgcat
    21721 tggtgcgggg atcctcgatg cgctgatcgg caccatgtca ccgctgatcc cggcgattat
    21781 cggcggatcg atggtcaaac tgctggcgat gatcctcgag atgagcggcg tgctgacaaa
    21841 aggatcgccg accttaacca ttctgaatgt gattggtgac ggtgctttct tcttcctgcc
    21901 gctgatggtc gcggcatctg ccgccatcaa atttaaaacc aatatgtcgc tggcgattgc
    21961 catcgcgggt gtgctggtac atccgagttt tattgaactg atggcgaaag cggcccaggg
    22021 tgaacatgtc gaatttgccc tgatcccggt caccgcggtg aaatatacct acacggtgat
    22081 cccggcgctg gtgatgacct ggtgcctgtc atatatcgaa cgctgggtgg atcgcattac
    22141 accggcggtg accaaaaact tcctcaaacc aatgctgatt gtgttgattg ccgcaccgct
    22201 ggcaatcctg ctgattggcc cgattggtat ctggatcggt agcgccattt cggcgctggt
    22261 ttacaccatt catggttatc tgggctggct ttcagtcgcc attatgggcg cgctgtggcc
    22321 tctgctggta atgaccggga tgcaccgcgt ctttacgcca accatcattc agaccattgc
    22381 cgaaaccggc aaagaaggga tggtcatgcc gtcagagatc ggcgctaacc tgtcgctggg
    22441 cggttcatca ctggcagtgg cgtggaaaac gaaaaacccg gaactgcgtc agacggcgct
    22501 ggctgcggcg gcatcagcca ttatggcggg gatttccgaa ccggcgttat atggcgtggc
    22561 gatccgcctg aaacgtccgc ttatcgccag tcttatcagc ggttttattt gcggcgcggt
    22621 tgccggtatg gcggggcttg ccagccactc aatggcagcg ccggggctat ttaccagcgt
    22681 gcagttcttc gatccggcga atccaatgag catcgtctgg gtgttcgcgg tcatggcgct
    22741 ggcggtggtg ctgtcgttta tcctcacact gttgctcggc tttgaggata ttcctgttga
    22801 ggaagcggct gcccaggcgc gaaagtatca gagcgtacaa ccgaccgtcg ccaaagaagt
    22861 aagtcttaat tgaggatgaa aatgtcagta tttccagaaa gttttttatg gggcggcgcg
    22921 cttgccgcca accagtctga aggtgcgttc cgtgaaggtg acaaaggtct gacgactgtc
    22981 gatatgatcc cacacggcga gcatcgaatg gcggtgaaac tggggctgga aaaacgtttt
    23041 cagttgcgag atgacgagtt ttatcccagc catgaggcga cggattttta tcatcgttat
    23101 aaagaagata tcgccctgat ggcagagatg ggattcaagg ttttccgtac ctcaattgcc
    23161 tggagccgtc tctttccgca gggcgatgaa atcacgccca atcagcaggg cattgctttt
    23221 tatcgttctg tctttgaaga gtgtaaaaag tacggtatcg aaccgctggt cacgttgtgc
    23281 cacttcgatg tgccgatgca tctggtcacc gaatatggct cctggcgtaa ccgcaagctg
    23341 gtggagtttt tcagccgcta cgccagaacc tgctttgaag catttgatgg tctggtgaaa
    23401 tactggctaa ccttcaatga aatcaacatt atgttgcata gcccgttctc cggcgcgggt
    23461 ctggtgtttg aagaaggtga aaatcaggat caggtgaaat atcaggccgc gcatcaccag
    23521 ctggttgcca gtgcgctagc caccaaaatc gcccatgagg ttaacccgca aaatcaggtg
    23581 ggctgtatgc tggcgggcgg taacttctac ccttacagtt gcaagccgga agatgtctgg
    23641 gcggcgctgg agaaagaccg ggaaaacctg ttttttatcg atgtgcaggc gcggggcacg
    23701 tatccggctt actctgcccg cgtattccgc gaaaaagggg taaccatcaa caaagcaccg
    23761 ggcgatgatg aaatcctgaa aaacaccgtc gattttgtct ctttcagcta ttacgcctcg
    23821 cgctgcgcct cggcggagat gaacgccaac aacagcagtg cggcgaacgt ggtgaaatcg
    23881 ctgcgtaatc cgtatctaca ggtgagcgac tggggctggg gaattgatcc actcggtctg
    23941 cgtatcacca tgaatatgat gtacgatcgt tatcagaagc cgctgtttct ggtggaaaac
    24001 ggcctgggcg caaaagatga atttgctgcc aatggcgaga ttaacgacga ctatcgcatc
    24061 agctacttac gcgaacatat ccgcgcaatg ggcgaagcga ttgcagacgg cattccgctg
    24121 atgggctaca ccacatgggg ctgtattgat ttagtttccg cctctacggg tgaaatgagc
    24181 aaacgctacg gctttgtctt tgttgaccgt gacgacgcag gcaacggtac gctgacgcgc
    24241 acgcgtaaga aatcattctg gtggtataaa aaagtgattg ccagtaatgg ggaagattta
    24301 gagtaggtaa cagtgccgga tgcggcgtga acgccttatc cggcctacat gtcgcacgaa
    24361 ccatgaaggc ccgataagcg cagcgcatcg ggcaatctgg ggtttggcat cagtcaaaat
    24421 caaagaaacc attccggctt aacgccaaat cgggctgaaa gtgccttaat gtgggatatc
    24481 gtcagggatc gttgtccaga caaaatctga ctgaccaggg atttagagcc gatttcgttc
    24541 ttcaaatccg cataggtcag attatgttga tcaatcaacg tgcgaagtaa cgccacgcca
    24601 accggcattg ccgcaacggc tttgtcgaac tctgcaaact tttcgttgtt gttttcatac
    24661 tctgcaatgc ggctcgccag aaagtcgata agtggattgg tgtcatcttc ttcaatcaaa
    24721 tattcgacca gttccagtgc ttcacgataa tcatcttctg agtcactgcc tcccagaaag
    24781 gggactgcat tgaccagttc tcttgttgct ttaacagcgc gtgcagcatt agcagtcatt
    24841 ctttgttctc tctgtagtaa cgtgtcagtt tgtcatactc tgcgtgattc gttatgtgct
    24901 tcacaaagaa ccgcttatta acaaagttta tgtaggcaat aaccctaaga ttgttcccgc
    24961 cgacatccag aacgtaccat ttgttgcgat atctaaaatt gtcgaggttg ggaaacgccg
    25021 ttcgcatttc ttctggtgta gaaaaatccg tttccttaat aacccgatac aatgcctgaa
    25081 gagccagagc gtcgttagga tatttacgcg cagactcttc aaatggcgct ttagaaatga
    25141 tgtgcatttt taagttttaa cttccttata gtaagtaaac agccacatct gcgatgttct
    25201 atccttatgt gttcggaaac tatagctcat caaatccaag ttcacaatat gtaaacactt
    25261 gtgcgcgata taattacccc caaaatcctc atagccactc tgcaaaaagt cagttctggt
    25321 tcacccctca agcacatccc cctttatcaa gcaacgctgc gtctgcgcca gatcttccgt
    25381 ttgcgggtcg cgcccggcga tgggtttgag gacgagttta cggttggggg tataagtaaa
    25441 acgcgtgagc gcgggatagc ttaacttgcc aaaccagccg tgaacacgct gccatttggc
    25501 gtcttttacc tgatacagac tggttatcca gtaaccagaa ttaaactgca tatccagcaa
    25561 ttgcgtgtga ccattgcctt gcaaatccag caaatcatct acgccagttt tgcttgcggt
    25621 gtagaatccc ctgggttcaa agacacaggg acggccttcg cgcgtgaacg tcataagaat
    25681 taaatgtgca taaggagcca gaccattacc ggtacttggt aaccagataa ccagatcctg
    25741 aatgccgtta cgatcaagat cggcgcgata gagttgccct ttgcccgcta tgtttgccag
    25801 catacccaca ggcacagacc aggatttacc ggcgcgatct ttaccgttca attgccattc
    25861 actatcatca tttggcatct tttcaagcgt cacgccagca acgcccactt ttctaaaatg
    25921 ccaggtttgc tcggcatcat aaggcatggt caggagaggt ttcagcgccc gcttttgcgc
    25981 gtattcgctg tcactcattg gcagatattc ggtaaagctg gtggcctgtg caggcagcac
    26041 taagctcaat aaaaaagcgc acatccctgt tctgatattc atttcgctga attccctgat
    26101 ggagaattat tcttcttcca ccgctaactg cgcgaagccg ccattccctt cccagccttc
    26161 cagtcgttga taaacggttt ctaccgcatc tttaatcggc tgagtcatcg ggtagtaaaa
    26221 gccgacgata tccggctgga tgccgaggaa aatcacttcg ccaatgtctt ctttcaactg
    26281 gtcgataaga tagttcagcg gcatgttatg ggtagtcatc ataaacatct cggcgatatc
    26341 atccgggtcg atgatgcgga tttcgccggg gtttagcccc atatcagtag cgtcaacaat
    26401 cagtaaccgc gtagggcgca gttcacggat ggcgacgatg tcgttttccg gtgcactacc
    26461 gccatcaatc accacccagt tacctttcgg cgcggcggcg cacttttccg ccagcagcgg
    26521 gccagcgcca tcatcgccca tcatgctatt gccaacacag agtaaaacgt cagtcacgca
    26581 gtctcctcac catcaaatag atggcgtttt cctgatgaat atcatgcagc atacttaaca
    26641 gcattgtact ccattccttc tgctgcgaag tttgcgctgc ccgggcttta tcgaatgcgt
    26701 tggccagcat cggcacatgg ttaatgtcga tgacgatctc gccatatttc ggtacgcctt
    26761 ccattttgcg gcgggcttcg ctgcccgcct ccagagtggc aatccatgcc agatattcat
    26821 cccatgggca ggtgagcgcc gcttccaggc aatcgataac cccaaggtgg tgaccaatcg
    26881 ccaggctgta atagaccacc tgctgcgcct cggcgggcgt ggcatcgttc tcatcaataa
    26941 atttacggct cagttgactg aacaccacct tttcactcat cggatacgcg cctcttcaac
    27001 aacatgattc agatggctga caatctcgtt cagacgcgga tcgttttccg cttccagcca
    27061 gcgtgcaacc tgttcttcgc cctgccctaa ctgtgtaagg taatcatcgg caatctgacg
    27121 accgtaacga taacccgcca ggcgacgtgc ttcgcgatcc actttcacgc gcagcggctg
    27181 caccatatcg ccatgcagaa tctcagccgg ttgttcatcc tgctcacccg ggccacgggc
    27241 gtgaattttc tgctccagca ggccgagtgc cattgcaaag ccgtacagcg tggcggcagg
    27301 cgttggcggg caacccggaa tgtagacatc taccgggacg attttatccg taccgcccca
    27361 cacgcagtag agatcgtgga aaataccacc gctgttaccg caggcaccgt aggagataca
    27421 aattttcggg tccggcgcgg actgccacgc acgcagcgca ggggatcgca ttgcacgggt
    27481 gaccgcgccg gtaaacagta aaatatccgc atgacgcggt gaaggaacga ctttaatgcc
    27541 gaagcgttct gcatcaaaca gcggcgataa agtggcgaaa atttcgattt cgcagccgtt
    27601 gcagccgccg cagtccacgc ggtaaacata ggcagaacgt ttgatttttt tcagtaacga
    27661 cgccttcatg ctggcgatgg attcatccac cgtcatcggg accggaatgc cgttggcgtc
    27721 acgggggcct aataaattgc tcatcagatg gcctctttca tatggcgagt cagttcaata
    27781 cggtcggacg gcaccaggca tttctggcgc ttacattccg ggcaagtctc aaagctttcg
    27841 cggtggtttt ccgcgcggct gtcaccattg tgcttaagca gcgcaatggc gtagtcgatc
    27901 tctttctgga cggcgaaagg acgattgcag acgcgacagc tgcacagcac aaaacgggac
    27961 tgttgcagga aatcttcttt cttccacacc gccagttcgt actcttgcga cagtttgatc
    28021 gctgccgtcg ggcagacttc ttcgcagcgt ccacagaaga tgcagcgccc gagattaaac
    28081 tcccacgcca gctcaccggt ggcgaggtca gtttcaaccg ttaaggcgtt tgacgggcag
    28141 gcattgacgc aggccgcaca gccgatgcac tgctgcgggt tctgctccgg cttaccacgg
    28201 aagtttttat caaccgcaat cggctccagc ggataagacg aggtcgccgt gccggttttg
    28261 atgacttttt tgataaaggt aaacatggcg attccttatt tcagcggcga gtttttacgc
    28321 tcaatgctgt aacgctcgag ttctttgtac ggcaccactt tgctcttttt cttacgcaca
    28381 tcgaccacgg tcatgcggtc ggtacaggag tagcaagggt ccaggctacc gataatcagc
    28441 ggcgcatcgg aaacggtgtt accgcgcagc atgtagcgca gggtcggcca gttcgcgtag
    28501 gtcgcggcac ggcagcgcca gcggtacagc ttctggttgt cgccggtcat gctccagtgg
    28561 atatcgtcgc cgcgcggcgc ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta
    28621 aagccttcca ccatcagcgg accgcccggc aggttatcca gaccgtagtc gatcatgttc
    28681 agcgcggtat aaacttcgtt gatacgcact ttcagacggg aaataacgtc gcagccctgc
    28741 tcgctgtgga cttccattgg cagcaggcca taaccgacaa acgggtgatc ggcgcgagta
    28801 tcgcgggcgt gaccgctggc acggaccatc gggccgacgt tactgaagtc gcgagcgatt
    28861 tccgggtcca gacgaccaat gccgacagtg cgctgttcca tgttcggagt gctcagcagc
    28921 acatccacca gctcctgcac ttcacgacgc atctgttgtg ccagctggcg ggtctggatc
    28981 atgtcgtctt tcagcagatc gcgacgaatc ccgccgatca agttcaggcc gtaggtttta
    29041 cgcacaccgg taaggatctc tgccattttc atggaggttt cacgcacgcg gaagaactgc
    29101 ataaagccgg agtcgaagcc ggtaaagtga caggccaggc caaggttgag cagatgcgag
    29161 tgcaggcgtt ccacctccag cagaatggcg cggatcatct gcgcacgttc tggcacctga
    29221 atacccatcg cgttttctac cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc
    29281 ccgcacacac ggtcagagag gaaggtcact tcgttgtaac ccatacgggt ttccgccagt
    29341 ttttccatgc cgcgatgaac gtagaacaga cggtagtcgg cgtcgataat gttttcgcca
    29401 tcgacgaaca gacggaagtg gcccggttcg tcagaagtga cgtgcagcgg accaatcggc
    29461 acgacgttgt ttttcttgtc gcccagttcg ttgatgaact cgtaggtttc agcatcggtg
    29521 gtcggtgccg gacgctgacg ataatccatg ctgtctttac gcagcggata aagttcatcc
    29581 ggccagtcat ccggcagcac cagacggcgt tcatccggca gaccaaccgg aatcaaaccg
    29641 tacatatcgc gcacttcgcg ctcgccccac accgccgccg gaacgcgcgg cgtcacggac
    29701 ggatactccg gtttgttggc atcaacttcg acgcgaaccg ttacccagca cttagtcccc
    29761 ttctccatcg acagcacgta ataaacggcg taatgaccat tcagtttgcg ttcgtcgtta
    29821 ccaaaaagca ccgacagcca gcccccctgc ttgtagtaga gaaactccac cacttccggc
    29881 agatagttca cctttacggt gacagtcagc tgatctttgg tctgccaggc gtggtccagc
    29941 acgacgcccg gaaatgcctc attcagcgcg gcgagataat gttgacctaa tttttcttca
    30001 gacatgctca aactctcttt aatcacgccg ccagcaagga gacgaacgct aaaaatgcaa
    30061 agccaaaccc agtccaggta atgcgcggag taatatcaag acgcagacgg gccatgctgt
    30121 tttcgaacag cgcgataacc aggacgccga cgaccagttt gacgatggca atcaccagcg
    30181 ccagcagcag cccaccggcg gtgaaggttt ccatctgtcc ccacgggata aatacgccga
    30241 cgaacatctg cagcaccacc agctgtttca ggctgatacc ccatttcatg acgccaaagc
    30301 cgctgccgct gtattcagag agcgggcctt cctgcagctc ctgctcggct tccgccaggt
    30361 cgaacggcag tttgcccatt tcgataaagg tggcgaacgc acaggcacaa agcgccagta
    30421 ccagcgggat gctctggctc agcggccagt gataaacggt gtcggtgatg ttgctgatgt
    30481 tggtggaacc ggcaacctgt gcggcaaccc acagaccaag cagcaacatc ggttcgacca
    30541 gcacgccaag catcgcttca cggctcgcgc cgatagcggt aaacgggcta ccggtatcca
    30601 gaccggaaat ggcaaagaag aaacgcgcga tggcaaagag atacagtaag gtgatcaaat
    30661 cgcccagttg cggcaacgga gaaccaacgg tcaccaccgg cagcgcagta gcgatagtca
    30721 gcatgacgcc caccatcaca tacggcgtca ggcggaaaac ccagccggag gcatccgggc
    30781 caacgctctg gcgacccagc agtttgaaaa tgtcgcgata ctcctgcaac acgcccggcc
    30841 cgcgacggtt atgcaagcgg gcgcgcgcca cgcgggttat accggagagc agcggcgcaa
    30901 cggcaaataa caccagcgcc tgaattaacg gatataaaac actcattctc aggctcctcg
    30961 tgaaacaata atcaccacca gtaccgccag ttcaaccagc gccatccggc ggaacagcaa
    31021 cgcactcccc tcgcactgcc agcccggcac cagcgacacc ggatttagcc atttgcgcag
    31081 tttcagcacc ggcgcaaacg cctgtttcac cggcatggca aaaccgtgag cggtaatcac
    31141 cattgatttt tcgtggtcgt agccgcacac ccaggccgcg ccgcgggaac gcgatggcaa
    31201 acgatcgcct ttgcaaatcg ccataatgat gaatggcagc agcgggcagg caatcagcag
    31261 caacgtgatc atcggttgag aaacggtggt gttagcaggc tccagcggca gaggtacagc
    31321 agcagacagc atcggcagca gccacggcgc agcaacaccg ccaattacgc agcaaatcgc
    31381 cagtgccact acgcttacgc tcatcaggag cggtgcacag gtggcgtttt cggcttcttt
    31441 ggtacgcggc gcgccgagga acgtgacgcc atagactttc gccatacaca tcaccgccag
    31501 cgcaccggta attgccagac ccacggcgag cagcggcccc agcagacggg caacaaacgc
    31561 gccactattg ctcagtttga agaaggattg atagataacc cattccccgg caaaaccatt
    31621 cagcggcggc agcgcagcca ttgccatcag cccgactaac atggcgatgg agataaccgg
    31681 cattttcttg ccaataccac cgagtttttc gatatcgcga tgaccggtac ggaaccagac
    31741 gctccccgct ccgaggaaca gtacgctttt gaacaggcta tggttaagca gatggtacag
    31801 accaccgacc aggccaagag caatcagtgc cggttgttcg agcgcgatac ccgttacgcc
    31861 agcgcccagc cccagcagga taatgccgat attttccagg gtgtggtaag ccagcaggcg
    31921 ctggatgttg tgttccatca acgcatacag accaccgacg aacgcggtga tcatgccgag
    31981 caccagcagc gcgatccccc accacagcgg tgcattaccg cccagcagtg acagggttaa
    32041 aatgcccagc aggccaattt tcatgactac cgtagaaaac aacgcggcag ctggcgcaga
    32101 ggcgttagca tgtgcctgcg gcacccagcc gtgcagcgga ataatcccgg ccagcaggcc
    32161 aaagccaatc acaccgagca gccagatatc ggaaccgagc ggcagctgtt gcatacgcat
    32221 atccagcagg cgcagatcca gcgtgccata acgctgccac agcagccagc aagcaatcgc
    32281 cagcagcaga gtgccaagac gccccagcgc aaaccacagt ttgccctctt tgctgttgct
    32341 ggtgaggaac accgcgcaca gggccatgat ttcggccatt accacgaaca tgccgaggtt
    32401 gctggcaatg acggcgcaga cggcggcagc catcaacata ttgatctgca agccgttgca
    32461 cttcacctgc gcgtggcgat gccagtcaat gttgtagagg ctgacaaaca gaccgcacag
    32521 accgagcgta atcagccaaa tcgcgttaag cggagagatt tgcacatcgt agcttaccag
    32581 cgacagcgca ccgctcacgc caaccgcgcc agtcagtaca gtgaagcccg cggctgccgt
    32641 atacagacta ccaaccgcgc cgccaattcc agctatccag ccacttaacg ctttttgaaa
    32701 agaaaagaga aatgccagaa cagcggcggc gacaaaccac gccacgccgc tattgatcag
    32761 ggaaattgcg ctcatttagc ctctccactt tgagcctgct gaaacaaggt gagatcgcca
    32821 aagtccgtgt taaaagtcag ctcacgctta cgtttgctga cgcgggcgat atcagtgtta
    32881 tccaccagat gcagggcttt agtcgggcac atccgcacgc aggccggacc ttgttcatca
    32941 aagctacaaa ggtcacattt gacggcgatc gcgcgaatac ctggcaccca gtcaagcaat
    33001 gtgctgacac gcgccggagc aggcggtgcc ggtggcgctt tcggggtatt ggcgtttgcc
    33061 ggaatatcca gcggacggct gccggaaaat tcaattgcgc caaacgggca ggcgatgccg
    33121 cacagcttgc agcttacgca caggctttca ttcaactgca cggccccatc aacgcgggtg
    33181 atggcgttaa ccgggcagac caccgcgcag ggtgcatctt cacagtggtg acagagctgc
    33241 ggcgcagatt ctttttcatt cagcatcact ctcaggcgcg gcattgattg caggccgtgc
    33301 tggcgatgcg tctctgaaca ggcggcctca caagtgtggc agccgataca gagcgtggag
    33361 tcagcaatta caaaacgatt caccaggcat tcctcaggtg attgtcattt ttgacgaaaa
    33421 catgccgatg aaatgtcatt ttcgacactc atcgacacgc ccatccccaa acaggcgtaa
    33481 cgcctgcaaa acgggcaaag cctcagctca tgctgccggg ctttgtccct ttaccagttg
    33541 gcttaaattc accggcacat tgttttcaat agcggtgtat aagctgtcgt aaacgccagc
    33601 gattttttcg agatagtgtt ccagaacctg ttcgcgatcg cggatgttga ccttgccatc
    33661 aatcatgccg tcaatgctca gttgcgcctg cgcaatccgc tgtttgtctt cgccatcttt
    33721 tgtttcgacg taatactcga tggtgtggct gttaaagcca tccgccaggg tgacgtaaat
    33781 gcgaaaatgt tcaaaaagga cgaaacagag ttctttgtcc ggcgcgcagc tcatggcgtg
    33841 atgcaggcgc gctttcgata acgtgatccc ctgaaccagc gaattgcagt agatgtgcca
    33901 ctggtcctgt aggcgacgat gccgctgtgc gatgtaatcg gctttctcgc ttatttccca
    33961 aatagtcatt gtcaggttac ccgtttaaca gagatgccag ctttaagcat tttttgtgcc
    34021 aacttttaat ttattgttat taaagagatt tttaagctaa agatgaattt cgtcgccgtg
    34081 tcgacgtgtc atttcgacat catcgacatt attcaccgca gggataatca acactggcac
    34141 aattattgct tgtagctggc aatagttaat gggaggcgat atgcacgaaa taaccctctg
    34201 ccaacgggca ctggaattga tcgaacagca ggccgcaaaa cacggcgcaa aacgcgtaac
    34261 tggggtctgg ctcaaaattg gcgcattttc ttgtgtcgaa accagctctc ttgccttttg
    34321 ttttgatctg gtttgccgcg gcagcgtggc ggaaggttgt aaactgcacc tcgaagaaca
    34381 aga
//
LOCUS       DAOFQD010000044        34305 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.044, whole genome shotgun sequence.
ACCESSION   DAOFQD010000044 DAOFQD010000000
VERSION     DAOFQD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.044"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            346..1644
                     /gene="kgtP"
                     /locus_tag="QBF46_002122"
     CDS             346..1644
                     /gene="kgtP"
                     /locus_tag="QBF46_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDQ6674302.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1641..1964)
                     /locus_tag="QBF46_002123"
     CDS             complement(1641..1964)
                     /locus_tag="QBF46_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDQ6674303.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDHSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2010..3365)
                     /gene="pssA"
                     /locus_tag="QBF46_002124"
     CDS             complement(2010..3365)
                     /gene="pssA"
                     /locus_tag="QBF46_002124"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDQ6674304.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3479..6139)
                     /gene="pat"
                     /locus_tag="QBF46_002125"
     CDS             complement(3479..6139)
                     /gene="pat"
                     /locus_tag="QBF46_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDQ6674305.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVEH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAVLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6171..6869)
                     /gene="tapT"
                     /locus_tag="QBF46_002126"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6171..6869)
                     /gene="tapT"
                     /locus_tag="QBF46_002126"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDQ6674306.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     YYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAPAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6938..7357)
                     /gene="trxC"
                     /locus_tag="QBF46_002127"
     CDS             complement(6938..7357)
                     /gene="trxC"
                     /locus_tag="QBF46_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDQ6674307.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7564..8601
                     /locus_tag="QBF46_002128"
     CDS             7564..8601
                     /locus_tag="QBF46_002128"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDQ6674308.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8649..9338)
                     /gene="ung"
                     /locus_tag="QBF46_002129"
     CDS             complement(8649..9338)
                     /gene="ung"
                     /locus_tag="QBF46_002129"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDQ6674309.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            9643..10026
                     /gene="grcA"
                     /locus_tag="QBF46_002130"
     CDS             9643..10026
                     /gene="grcA"
                     /locus_tag="QBF46_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDQ6674310.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10082..10669)
                     /gene="eamB"
                     /locus_tag="QBF46_002131"
     CDS             complement(10082..10669)
                     /gene="eamB"
                     /locus_tag="QBF46_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDQ6674311.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10772..11653
                     /locus_tag="QBF46_002132"
     CDS             10772..11653
                     /locus_tag="QBF46_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6674312.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRSTVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFLPG"
     gene            complement(11686..13020)
                     /gene="srmB"
                     /locus_tag="QBF46_002133"
     CDS             complement(11686..13020)
                     /gene="srmB"
                     /locus_tag="QBF46_002133"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDQ6674313.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13152..13889
                     /gene="trmN"
                     /locus_tag="QBF46_002134"
     CDS             13152..13889
                     /gene="trmN"
                     /locus_tag="QBF46_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDQ6674314.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVVIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13874..15496)
                     /gene="nadB"
                     /locus_tag="QBF46_002135"
     CDS             complement(13874..15496)
                     /gene="nadB"
                     /locus_tag="QBF46_002135"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDQ6674315.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15752..15907
                     /gene="rseD"
                     /locus_tag="QBF46_002136"
     CDS             15752..15907
                     /gene="rseD"
                     /locus_tag="QBF46_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDQ6674316.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLILCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15904..16479
                     /gene="rpoE"
                     /locus_tag="QBF46_002137"
     CDS             15904..16479
                     /gene="rpoE"
                     /locus_tag="QBF46_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDQ6674317.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16512..17162
                     /gene="rseA"
                     /locus_tag="QBF46_002138"
     CDS             16512..17162
                     /gene="rseA"
                     /locus_tag="QBF46_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDQ6674318.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17162..18118
                     /gene="rseB"
                     /locus_tag="QBF46_002139"
     CDS             17162..18118
                     /gene="rseB"
                     /locus_tag="QBF46_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDQ6674319.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18115..18594
                     /gene="rseC"
                     /locus_tag="QBF46_002140"
     CDS             18115..18594
                     /gene="rseC"
                     /locus_tag="QBF46_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDQ6674320.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18792..20591
                     /gene="lepA"
                     /locus_tag="QBF46_002141"
     CDS             18792..20591
                     /gene="lepA"
                     /locus_tag="QBF46_002141"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDQ6674321.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20607..21581
                     /gene="lepB"
                     /locus_tag="QBF46_002142"
     CDS             20607..21581
                     /gene="lepB"
                     /locus_tag="QBF46_002142"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDQ6674322.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21854..22534
                     /gene="rnc"
                     /locus_tag="QBF46_002143"
     CDS             21854..22534
                     /gene="rnc"
                     /locus_tag="QBF46_002143"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDQ6674323.1"
                     /translation="MNPIVINRLQRKLGYTFNHPELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22531..23436
                     /gene="era"
                     /locus_tag="QBF46_002144"
     CDS             22531..23436
                     /gene="era"
                     /locus_tag="QBF46_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDQ6674324.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23448..24176
                     /gene="recO"
                     /locus_tag="QBF46_002145"
     CDS             23448..24176
                     /gene="recO"
                     /locus_tag="QBF46_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDQ6674325.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24188..24919
                     /gene="pdxJ"
                     /locus_tag="QBF46_002146"
     CDS             24188..24919
                     /gene="pdxJ"
                     /locus_tag="QBF46_002146"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDQ6674326.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24919..25299
                     /locus_tag="QBF46_002147"
     CDS             24919..25299
                     /locus_tag="QBF46_002147"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDQ6674327.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25720..25800
                     /gene="shoB"
                     /locus_tag="QBF46_002148"
     CDS             25720..25800
                     /gene="shoB"
                     /locus_tag="QBF46_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDQ6674328.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25994..26254)
                     /gene="yfhL"
                     /locus_tag="QBF46_002149"
     CDS             complement(25994..26254)
                     /gene="yfhL"
                     /locus_tag="QBF46_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDQ6674329.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(26310..27158)
                     /locus_tag="QBF46_002150"
     CDS             complement(26310..27158)
                     /locus_tag="QBF46_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDQ6674330.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27367..28002
                     /gene="yfhb"
                     /locus_tag="QBF46_002151"
     CDS             27367..28002
                     /gene="yfhb"
                     /locus_tag="QBF46_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDQ6674331.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            28027..28563
                     /gene="tadA"
                     /locus_tag="QBF46_002152"
     CDS             28027..28563
                     /gene="tadA"
                     /locus_tag="QBF46_002152"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDQ6674332.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(28560..30116)
                     /gene="mltF"
                     /locus_tag="QBF46_002153"
     CDS             complement(28560..30116)
                     /gene="mltF"
                     /locus_tag="QBF46_002153"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDQ6674333.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30374..34261
                     /gene="purL"
                     /locus_tag="QBF46_002154"
                     /gene_synonym="purI"
     CDS             30374..34261
                     /gene="purL"
                     /locus_tag="QBF46_002154"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDQ6674334.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLKDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT         8273 a         8608 c         9134 g         8290 t
ORIGIN      
        1 agagtaggga actgccaggc atcaaattat gcgaaaggcc atcctgacgg atagcctttt
       61 tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg
      121 ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta
      181 ccagaaattt atccttaagc tcctcaataa ccattttcct gctaactaaa ttcatggtta
      241 aggttgcata atgatatgca acaaatgtat aacatttcct ttaccaaaaa aaataaacaa
      301 aagcgaccga caaaagcatc ggattacggc aggagacata atggcatggc tgaaagtact
      361 gtaacggcag acagcaaact gacaagtagt gatactcgtc gccgcatttg ggcgattgtg
      421 ggggcctctt caggtaatct ggtcgagtgg ttcgatttct atgtctactc gttctgttca
      481 ctctactttg cccacatctt cttcccttcc gggaacacga cgactcaact actacaaaca
      541 gcaggtgttt ttgctgcggg attcctgatg cgcccaatag gcggttggct atttggccgc
      601 atagccgata aacatggtcg caaaaaatcg atgctgttat cggtgtgtat gatgtgtttc
      661 ggatcgctgg ttatcgcctg cctcccaggt tatgaaacta taggtacgtg ggctccggca
      721 ttattgcttc tcgctcgttt atttcaggga ttatctgttg gcggagaata tggcaccagc
      781 gccacctata tgagtgaagt tgccgttgaa gggcgcaaag gtttttacgc atcatttcag
      841 tatgtgacgt tgatcggcgg acaactgcta gccctactgg ttgtcgtggt tttacaacac
      901 accatggaag acgctgcact cagagagtgg ggatggcgta ttcctttcgc gttaggagct
      961 gtgttagctg ttgtggcgtt gtggttacgt cgtcagttag atgaaacttc gcaacaagaa
     1021 acgcgcgctt taaaagaagc tggatctctg aaaggattat ggcgcaatcg ccgtgcattc
     1081 atcatggttc tcggttttac cgctgcgggc tccctttgtt tctatacctt cactacttat
     1141 atgcagaagt atctggtaaa tactgcggga atgcatgcca acgtggcgag tggcattatg
     1201 actgccgcat tgtttgtatt catgcttatt caaccactca ttggcgcgct gtcggataag
     1261 attggtcgcc gtacctcaat gttatgtttc ggttcgctgg cagccatttt taccgttcct
     1321 attctctcag cattgcaaaa cgtttcctcg ccttatgccg cttttggtct ggtgatgtgt
     1381 gccctgctga tagtgagttt ttatacatca atcagtggaa tactgaaggc tgagatgttc
     1441 ccggcacagg ttcgcgcatt aggcgttggt ctgtcatatg cggtcgctaa tgctatattt
     1501 ggtggttcgg cggagtacgt agcgttgtcg ctgaaatcaa taggaatgga aacagccttc
     1561 ttctggtatg tgaccttgat ggccgtggtg gcgtttctgg tttctttgat gctacatcgc
     1621 aaagggaagg ggatgcgtct ttagtgacgg gtcagttgcc agacggtata gccggtgctt
     1681 gcaccggcga catcccaggc caaatccttc cagctccagc cgctcccttc ggggcggcta
     1741 tcccaaagct cttttgacgc ccccaaactg acagagaaca tcaatccaaa catggcactg
     1801 tgatcccggc tcatcccctg atgctgtgaa tattcatttc cggcggcgga aagcatcgcc
     1861 gaggcgataa agtgttgagc tttatcctgc ccgctccagc tatcgttagc catatggctg
     1921 catccagaaa gtaacaatag cgaacagaca aaaagaatac gcaaaacgtg gcctccaaaa
     1981 aacaaacccc gtacaggacg gggttgtgat tacaggattc ggctaattaa tcggtcgatg
     2041 cggatacggc gcaaacggcg gatgagttta cgaaccttca ccggataatc ggcaatactt
     2101 tgcagatcgc gatagtgctt aacgatggtg gtatgctcgc ggatcagctc cagttctttc
     2161 tctcgctgcg gcgcaagctc cagttgtgga tcgtggatca aaatggcgtt ttccagatcc
     2221 agacgccagg cgcgcgggtt caggttatta ccggtgatca acatccactt atcatcaacc
     2281 cacatccctt tcaggtgata ggtgttgtcg tcatctttcc ataaccgaac cactaactgg
     2341 tcagtattga cgtaatactg caaacggctc aggaaacgac gcagattgat ctcatagaga
     2401 taaggcaatg cgccaattat cttgaaaggt tcatcttccg gaatgtagaa gtcgttcgcg
     2461 gttttatcac caacaataat ttcgaccttt ttcccttcgc gcagcaactg gataatattg
     2521 cgcacaagga ttgctggcag gttgaagtat ggcgtacaga tggttagttt ctgctcggca
     2581 caaggcataa gatggaaaat ggtcttgttc aacagactcg atttccccag ccccactagc
     2641 ggcgttacgg aaagctgatc gttgtcggca tcgccctgga aatgataagc ggcatcacgc
     2701 agctcctggc ggaacagacg aatatcgttc ttgatttccg ggctttttgg ccgattaaca
     2761 tcatccagac gattaacgcc gcggccattc ataatattct gtgtaaccca ttcaaacata
     2821 atgtctgaca tcttacggtt acggataaga tgataacggt cgtagcgata tttatcgtgc
     2881 tgatgcaggt aaacatcgtt caggctggca ccgctataaa gtacgctatc gtcgatgata
     2941 aagcctttaa agtgcagaac accaagggct tcacgggtat tgattggaac gccataaacc
     3001 ggaacatcta cgcccggatt ttcctgcgcc atgcggcagt accagtcagc gttagtgtta
     3061 gatgccgcag cgccaatgcg tccacgttgt gcacgatgcc agtcgaccag cacccgcaca
     3121 tccagttccg gacgctgcct tttagcctca tacaacgcgt tcagaatgcc tttgccaccg
     3181 tcatcctgtt cgagatacag ggcgacaatg caaatgcgct gcttcgcgct ggctattttt
     3241 tccagcagcg tctcccggaa gtcggcggga gcgtaaaaga aatcgacatc atcaactgat
     3301 tgagaaatct tgggtagttg ggcaaggtgt tgttgatgtt tattacgctt aaattttgac
     3361 aacatcacag tgcatttctt ctctgttcat tgaagggtcc tctgtgcaat gcagacgaca
     3421 taagcgggca ataataacac cagtcccgat taagtggtca acatttccag taccttactc
     3481 atgattcctc gcgctgggca agatttagcg taagcccaac gatcccctct tcgagctgga
     3541 tatcaacgtt aaacccgagt ttgcgggcca gcgccaccat gccacgattg tttggcatcg
     3601 taataccatt cagacgttgt agtccgtgat ctcgcgtata ggtaatcaac ttttccatta
     3661 agcgtcgacc taagcctaac cctttgagat ccgagcgaac cagtacagca aattcggcat
     3721 cgatgttatc aggatcggaa atcgcacgcg tgacgccgag gatctcttcc gtttgatcaa
     3781 tacgtcgtac cgctacaaac gccatttccc gatcgtagtc gatctgtgtc atgttggcta
     3841 aatcttcatg ggtaaattcg ttgatctcgc taaagtagcg gtaataaaga tcttctttgg
     3901 tgactcgcga aatgaattgc tgaagttgtg gctcatcttc tggcaaaatc gggcggaaca
     3961 agcagcgttc accgtttttc aattctaccc attcttccag ctgatgcgga taagggcgca
     4021 ctgccagccg actctcgtta tcgccttcaa acggcgagat atccagcgtg acatccagcg
     4081 cggtaaattc actgccagaa gccagcaaag gatgaatatc cagacgctga atttccgggc
     4141 aatcgacaat caagttggaa acctgcacca gaagctggct caagcctgca acatccaatg
     4201 ggcgtagcgc actgcgcgca cgaatctttt tacttttgat cccctgaata accagatagc
     4261 gggccaggtt catgttcagc ggcggcagtg cgacgacggc ttgatcttca ggacgccact
     4321 ccacaccgcc ttcacccagc atgatcaacg gcccgaaaac cggatcgtgc tcaaccacaa
     4381 cccgcaactc ctgagcgcca gcacggttag ccatactttg caccaacagg ccgtggaccc
     4441 gcgcctgtgg ccaggccatt tttacgcgat cgaaaatagc gttcgccgct tgctggactt
     4501 cattggctgt acgcaggtaa agcatgacgc cctgaacttc cgatttatgt ggaatatccg
     4561 gcgaacgcaa tttcagcgcc accggataac caatctgttc ggcaatatgc accgcttcgg
     4621 tgctatcgct ggcaatccag gtagggagcg tgttcatgcc atacgcttgc aggatgggct
     4681 gaacttcatg ggtatcgagc gacgtagccc cttcggcaat cgcctgttgc aacagaagat
     4741 gcgcttctgc ggtattggaa gtcagattgc tgggcaacgc cggcgtttcg cgtagttgct
     4801 tctgattacg ccggtactcc accatatgca taaaagcagt gatggttcct tccggggtac
     4861 ggtaggtcgg cagcacggct tcgctgaata aacgtcgtgc ctcttgcgag gagtgctcgc
     4921 cgcaccagtt cgtcagcaaa gagacatatt tgctgcgggg atgatgctct accgcttcaa
     4981 ttaatacttg cgcgctttct gttgcgggag cagcggcgct gggcgaatga ataaccatca
     5041 gcgcgtcaaa atcctggctg tggagcagaa tatccagcgt tttaatatag tgctcactgc
     5101 tggcgtcatc gcgtagatcg agcgggttag atattgccac atgttctggc agtgcatcgc
     5161 gcagtttctg gcaggtttct tcgcttagcg ttgccagctt gccattgcgt gaccataagg
     5221 catccagcgc cagcgcggca ggcgcagcac cgttgctgat aatcatcagc cggtcgccac
     5281 gtagtgggcg catatggcta agggtttcca ccgccgaaaa cagctcgtgg gtgtcctgta
     5341 cccgcaacaa accggcacgc tgaatagccg catcccatgc cgggtccatt cctgccgtcg
     5401 tgttgagcag tcgctgtgcc gccgggctac gtccgctttt aatcaccaga atcggtttat
     5461 tacgcgaggc actacgggcc gccgaaacaa agcgtcgcgc gtcgcttaac tgttcgagat
     5521 agagcaggat ggcgctggtt ttactgtcgc gtgccagata gtcaagcaat tcatcaacgt
     5581 cgatatccag gctgtcgccg agcgcaataa agtaggaaaa gcccatctta cgctgttgcg
     5641 cccagtcgag gatggtgttg gagacggcag ccgattgcga aataaacgcc agcttgccgc
     5701 gtttaatcgg cacaggcgaa aagctggcat tcagaccttg ccagggagcc agtaaaccca
     5761 gactgtttgg tccaagcagg cgcatgttat ggcgcagggc gcaggcgcgg agatcttcgt
     5821 gttgcgatgc cggggcggaa agaataatgc aggttttaca gcctttctcg ccgagctctt
     5881 ccagaagagc aagattacgg ctggcattgg tacataaaac cgcaaggtcg ggtgtaaagg
     5941 gcaagctggc aatatccggc caggccaaca cacccaacac cgctttccag gctggcgtca
     6001 ccgggagtac cggtccgtta aagcctcccg ccagcaggtt acgcatcatc aggtaacctg
     6061 cgcgattggg tttcatcgac gcgccaatta ccgctatcga ttttggtcgc agtagtgctt
     6121 ccagtcctcg ctgactcata ccggtctccc gcagaagtga ccgaatgatt ttaaacgctt
     6181 tctaactgtt ctgctgtgat gctacccaga tgttgcgttt ttcctgccag atagcgtgtt
     6241 ttaaagcggg taaaatgctc gcctaaccct gctgccgccc cggtatcgcc ggccatatct
     6301 aacagtgcga tggctacctc ggcagtacaa tattggcctt cagccggggc ttcacgcagg
     6361 cgataggcag aaagccggga aagatcgacg gaaatgacgg gaagattatc cagatacgga
     6421 cttttacgaa acatcttgcg agcttccggc caggtaccat cgagcatgat aaacagcggt
     6481 ggcttaccgg caggtggtgt gaagatcact tcccgttgct catcagcata cgaggcggga
     6541 aagaccacca ttggctgata atacgggttt tgtaccagat ccagcaaatc ctgcgagggt
     6601 tcggtacgcg accattgaaa cgcaacggta tcaggcaaaa tatcagcaat cagacgcccg
     6661 gtattactgg gcttcattgg ctcggtgtcg aacatcagca aacagaagcg actttttgct
     6721 tgtgctgggg taattgtcga acagagacat aatttctctg gcaaaagaca gcgttggcag
     6781 cgacgaacgc gattaccgcg ggcaagaaaa ggacgtgttg cgcgcgcaat acgctcggcg
     6841 cgtaactgga gaacagcgtt ttcggtcata agagagcgtc gaaaaaacgc cattgtcgca
     6901 gaggagaaaa cggggcacaa gatgcgcccc ggtaagatta aagagattcg ttcagccagc
     6961 tatcgaacgg cgctttcggt actgcgccat taagcatgtc gacaacctga ccgtttttga
     7021 aaatcatgat cgtcggtata ctacgaattc caaagcgact gctcaattca cgttcagctt
     7081 cggtattcac tttcacaaag cgcactttac cgctacgctc ttgcgcgaca tcttcaaaaa
     7141 ttggtgcgaa attacggcag gggccgcacc acggtgccca gaagtcgatc accacaggta
     7201 gatcatcctt cagcaatttg tcgagcgttt caccggtcgc attaatcacc tctccgtcaa
     7261 acaagtcgtg accgcagcgt ccgcattttg ccgcatcttc gatccgatcg tcgggaatgc
     7321 gattgatggc ctgacaatgg gtacaaacgg tattcataac taacctcggg atgagtaagg
     7381 agacttatcg gcaacgacgc cggtatgttt ctaatatgtt acatattatc ggggacattg
     7441 ttccagacaa caatctgttt tattcaatag gtatgatagt cgcaggcttt ggtacgaaat
     7501 catggcgaac cgcgcggact tcgggtaatc tgcgcgcttc gcgcagcgct ggtggagaaa
     7561 agcatgaacg atgaaatgaa aggtaaaagc ggcaaggtca aagtgatgta tgtccgcagt
     7621 gatgatgatt ctgataaacg tacccacaac ccgcgtaccg ggaaaggggg cgggcgtcca
     7681 ggaaaatctc gtgctgacgg tggccgtcgc cccgcccgcg atgacaaaca gagtcagccc
     7741 cgtgaccgca agtgggaaga ttcgccgtgg cgcacggttt cccgcgcgcc gggtgatgag
     7801 acgccggaaa aggccgatca cggtggcatc agtggtaaaa gttttattga tccagaagtt
     7861 ttgcgtcgtc agcgtgcgga agaaacccgc gtctacggcg aaaatgcatg tcaggcactg
     7921 ttccagagcc gtccggaagc gattgttcgc gcctggttta tccagagcgt aacgccacgt
     7981 tttaaagaag ccttgcgctg gatggcggca aaccgcaaag cgtaccatgt ggtggatgaa
     8041 gcggaattga caaaagcgtc aggcacggaa catcacggcg gcgtttgctt cctgatcaaa
     8101 aagcgtaatg gcacaaccgt gcagcagtgg gtaagccagg caggtgcgca ggattgtgtt
     8161 ctggcactgg aaaacgaatc taacccgcat aacctgggtg gcatgatgcg cagctgcgcg
     8221 cacttcggcg tgaaaggcgt tgtggtgcag gatgcggcac tgctggagtc gggtgcggct
     8281 atccgtaccg cagaaggcgg cgcagagcac gttcagccga ttactggcga caacattgtt
     8341 aacgtgctgg atgatttccg tcaggcgggt tacaccgtag tgacaacttc cagcgagcag
     8401 gggaaaccgc tgttcaaaac cagcctgcca gcgaaaatgg tactggtgct gggtcaagaa
     8461 tatgaagggt taccggatgc cgcacgcgat ccgaacgatc tgcgcgtgaa gattgacggt
     8521 acgggcaacg ttgccgggct gaatatctct gttgcaacag gcgttttgct tggtgaatgg
     8581 tggcgtcaga ataaagcctg ataaatcagc cgggtggcaa ctctgccatc cggcattttc
     8641 ccgcaaattt actcactctc tgccggtaat actggcatcc agtcaatcgg cgtctcgcca
     8701 tgttgttcca gccactgatt tgccagcaca aaatggttgc agccaaagaa tccacgatgc
     8761 gccgaaagcg gcgacggatg cggtgctttc agtacatgat ggcgttgctt atctataatc
     8821 gcccctttct tttgcgcatg cgatccccac aacaaaaaca ccacgccttc gcgatgctgg
     8881 ttaatcaggc tgatcacttt atcggtgaag gtttcccagc cgaggctggc gtgggaatgc
     8941 gcctgacctg cgcgtaccgt caacacagta ttgagtagca gaacgccctg acgcgcccag
     9001 ctttcaagat aaccatgatt agggcgggtg aagcccggaa tagtattttc cagctcttta
     9061 tacatattca ataatgacgg aggaatggca atgccgggac gaacggaaaa tgccagacca
     9121 tgcgcctgtc ccggtccgtg ataaggatcc tggccgagaa tcaccacttt aacgtcaccc
     9181 aactctgtaa agcggaacgc gttaaagaca tctttttgtg gtgggtagat agtgacgccg
     9241 gactgccgct cgctggcgac ggtctgaagg gtattaagaa aatagggttg ctgcttctct
     9301 tcagccagca cgtcatgcca ggttaattcg ttagccatct cgctctcctg cgaatcttca
     9361 atccgcctag cttaactgct tctttctaca gaacaaaatc atgctcattt tttagactta
     9421 aacgagtgca aaaaaatgta aattggtaaa atttcacacc cgcataagtt gatgtaaaac
     9481 aataaaatcc atttgcgacc atacttgatg tgtggttttt attgatttaa atcaaagatt
     9541 caagggtgtt tgaggagtat atatacactc aagcaacaat ggttttacca attggccgcg
     9601 acaggctgaa caaatcaaat aattttgccg gggaggcatc acatgattac aggtatccag
     9661 attactaaag ccgctaacga cgatctgctg aactctttct ggctgctgga cagcgaaaaa
     9721 ggcgaagcgc gttgcatcgt tgcaaaagca ggttatgcag aagatgaagt ggttgcagta
     9781 agcaaactgg gtgacattga ataccgtgaa gttccagtag aagtgaaacc agaagttcgc
     9841 gttgaaggtg gtcaacacct gaacgttaac gttctgcgtc gcgaaactct ggaagatgca
     9901 gttaagcatc cggaaaaata tccgcagctg accatccgtg tatccggtta tgcagttcgc
     9961 tttaactctc tgactccgga acagcagcgc gacgttatcg ctcgtacctt tactgaaagc
    10021 ctgtaaggct taagtagcaa gttactaagc ggaagagggc gacctcttcc gctttttttc
    10081 gttaatagaa aatgcgtacc gcgcaataga ccagcaacag ggcaagcacg atatttaact
    10141 ggcgaccata ctggcgaaac aatcgctgaa acagatgccc cgccagcgcc cagcacacat
    10201 tgccaaacgt cccaatcatc gccagcaaaa cgctgacgcc aactacccag cttagcgcct
    10261 gtgtttgcgg cagaacaaac gtcgacagtg ccgtaacacc gtacaaaatg attttgacgt
    10321 tcacaaactg caaagcaaag ctggcccaaa agctgattgg ttttgcctga agtccgtctt
    10381 cctttgttgg gctggtggcg attttccacg ccagccagac aatatatgcc gcccccgccc
    10441 aactcaaaag gtgtaccgct gccgggtcaa tcactgccag tgaaaatgaa atgcccgcac
    10501 acagtaacat cacaatcaaa aatcccagac tcatccctgc cagcacgcgg gtactttgac
    10561 gaaatccatg cgacgtagca gagctaaggg cgagaatatt gttcggtcct ggcgtcatag
    10621 cggtaatcag ggtgtaagtc caaaaagcac ttaaaagggt cggtgtcact tgttatctcc
    10681 tctgttttgg tagacagtac ccgacacgga acatttaaaa aaatgaatgt ttttatattc
    10741 tagattcgat attttcgata ggtttggggt tatggatctg cgccgtttta ttacgcttaa
    10801 aaccgtggtg gaagagggtt cctttttgcg agcttcgcaa aaattgtgct gtacacaatc
    10861 gacggtgact tttcatattc agcagcttga gcaggagttt tcagtccagt tatttgagaa
    10921 aattggccga cggatgtgcc ttacccgcga aggaaaaaag ttgctgccgc acatttatga
    10981 acttacccga gtgatggata cgcttcgtga ggctgccaaa aaggagtcag atccggacgg
    11041 ggaactacgc gttgtttcgg gcgaaacgtt gctctcttac cgaatgccgc aggtgttgca
    11101 gcgttttcgg caacgcgcac caaaagtgcg tttatcgttg cagtcgctga actgttacgt
    11161 gatccgtgat gcgctactga atgatgaggc cgatgtcggc gtcttttatc gcgtagggaa
    11221 tgatgatgcc ctgaaccgac gagagttggg tgaacaatcg ctggtgctgg tggcttcacc
    11281 gcaaattgca gatgtcgatt ttactgagcc gggaagacat aacgcctgta gctttattat
    11341 caacgaaccg caatgtgtct ttcggcagat ctttgaaagt acattgcgcc agcggcggag
    11401 cacggtggaa aacaccattg agctgttaag cattgaaagt atcaaacgtt gtgtcgcggc
    11461 gaatatcggt gtcagttact tgccccgctt tgcggtggaa aaagagcttg agagcggaga
    11521 attaattgaa ttgccttttg gcgaacagtc gcagaccatt acggcaatgt gcgcacatca
    11581 tgctggaaaa gcggttagcc cggcgatgca catttttatt cagtgtgttg aagagtgttt
    11641 tttgccggga taaaaataat gccgggctta aacccggcat tgagattact cttctgtcgt
    11701 ttgcggtggc acgcccgttc cgcttggttt acggcgctta ccaatatttt tggtatcgcg
    11761 atggcgtttt ttcacccgcg gcttctcttt ctctttagct tttttcttct cggcgcgttt
    11821 tgccagtact ttcttcgatg gcttgccggt ctgcttctcg cttggtgcac gcgttttcgg
    11881 gcgtaactca tcaattacgc gagctttaat tggctcttca atatagcgac caactttgcc
    11941 cagcagcaga tggtcatggg cttccaccag cgaaattgcg gtgcctttac gaccggcgcg
    12001 cgcggtacgt ccgatacggt gcaaataagt atcgccactg cgcggcatat cgaagttaaa
    12061 gacgtggctg acgtcaggaa tgtcgatacc gcgcgcggca acatcggttg cgaccagtac
    12121 gtttacgcga ccttcggtca aacgcttgat cgcttcgtta cgcttgccct gtaccatctc
    12181 accttcgaga tagcagttgt tgatgcccgc ttcgcgcagc cagtttgcca gctcatgcac
    12241 acgctcacgc ttacgcacaa acacaattga gcgggtcgct tccggctgtt ttaacagatg
    12301 caccagcaac gcggttttat gctcaagatc atcggcgcgg taataccact gatgaatttt
    12361 tttgcgctca cgggtggagg gattggcaga aacttccacc ggatcttcca gcagacgctc
    12421 ggcaaagtcc tgaatcgcat cgccttccag cgttgccgaa aagagcaggg tctgtttacg
    12481 ccagcgcgtt tcgccagcaa tatgttcgat atcctgagcg aagcccatat ccagcatacg
    12541 gtctgcttcg tcgaggatca gcgtttcaac cgcgcggcaa tcgaagttct cttcttttat
    12601 gtattgcagc agacgtccgg tcgtggcgac cacgatgtcc tgattttcgc tgaacacttc
    12661 cgcgtggttc atataggcta cgccgccggt gatggtggcg atatccagat gcgtatgttt
    12721 cgccagttcg cgggcatgat cggccacctg catcgccagc tcgcgagttg gggtgaggat
    12781 caaaatacgc ggcggaccgg atttcttacg cgggaaatcg agcaggtgct gcaacgctgg
    12841 cagcagatac gccgccgttt taccggtgcc tgtcggcgca gaaccgagta catcacggcc
    12901 atcgagcgca ggcggaatgg cggcagcctg aatggcggtc gggcgagtga aacctttatc
    12961 ctggagggct tccagcaggc tttcgtcgag ttcaagttcg gaaaaagtcg ttacagtcat
    13021 gttctacctc tgtgtggggc gctgattata gacgttacgg ctgcaatctt catctgtttg
    13081 tatggatatc gcttctcggg tattgttctc acccggtgct atcgccgttt ttcctgcaag
    13141 gttttatttt catgtcacag tctacatccg tgcttcgtcg taatggattt acttttaaac
    13201 agttttttgt tgctcacgat cgctgtgcga tgaaagtggg aacggatggt attttattgg
    13261 gcgcatgggc accggtggct ggggtaaaac gttgccttga tatcggcgcg ggtagcgggt
    13321 tgctggcatt aatgctggcg cagcgaaccg atgacagcgt ggtgattgat gcagttgaac
    13381 tggaaagtga agctgccgcg caggcgcagg aaaatatcaa ccagtccccg tgggcagagc
    13441 ggattaacgt ccatacggcg gatattcagc agtggatcac acagcagaca gtacgcttcg
    13501 atttaatcat cagtaaccca ccttactatc agcagggagt agagtgctcg acacctcaac
    13561 gggaacaggc tcgctatacc actacgcttg atcacccatc gttgctgacc tgtgcggcgg
    13621 agtgcattac cgaagaggga tttttctgcg tagtgctgcc agagcagatt ggtaatggtt
    13681 ttacggagct ggcattaagc atgggctggc atttacgttt gcggacggat gtggcggaaa
    13741 acgaagcgcg actgccgcat cgggtgctgc tggcattctc cccgcaggcg ggagaatgct
    13801 ttagcgatcg cttagtcatt cgtgggccag accagaacta ttccgaagcg tatacggcgc
    13861 tgacccaggc tttttatctg tttatgtaat gattgccggg ggaaaggatc gacggaccgg
    13921 aatgggtgag cagttccgga taatccagcg tgaaatgtaa cccccgactc tctttacgca
    13981 tcattgcaca gcgaacaatc aactcggcaa cctgtaccag attacgcagc tccagcaaat
    14041 tatttgagac gcggaaatgg gcgtaatatt cgtctatttc ttgttggagc atggttatcc
    14101 gccgcagggc gcgttccagg cgcttcgttg tgcgcacaat gccaacgtaa tcccacataa
    14161 acagacgtag ctcgtgccag ttatgctgaa ttactacccg ttcgtcaggg ttctcaacgc
    14221 ggctttcatc ccacggcggt aacgtactga tgtcgtgggc ataaggcata cgtctggtga
    14281 tatcttccgc cgccgaccag ccatagacca gacactccag caatgaattc gaggccatgc
    14341 ggttagcgcc gtgtaagccg gtataactca cctcgccaat ggcatacaag ccctcgacgt
    14401 ccgtacgccc atgatcatca accattacac caccgcaggt ataatgtgca gcaggcacaa
    14461 tcggtaccgg ttcttgtgtg agatcaatcc ccagcccgag cagcttttca taaatcatcg
    14521 ggaaatgctg gcgaataaaa tcggcgggct tatggctgat atcaaggaac atacaatctg
    14581 cgccgaggcg tttcatttca tggtcaatgg cgcgggcgac aatatcgcgc ggggccagtt
    14641 cgccgcgctc atcaaaatcg ggcataaaac gcgtaccatc cgggcgcttg agataagcgc
    14701 cttcgccgcg cagtgcttct gttaacagga aattgcgtgc ctgtgggtga tatagcgcgg
    14761 tagggtggaa ctgattaaat tcgagattgg caacccggca gcctgcgcgc cacgccatag
    14821 caatgccatc gccagaagaa atatccggat tggtggtgta ctgataaacc ttcgacgcac
    14881 cgccagttgc cagcaccacc gcttttgcgt ggcaggtttc caccgtttct ttattacggt
    14941 tccataccca cgcgccaaca acccgtcgcg tgcccggcag gccaattttg tcagaaataa
    15001 tcagatcaac cgcgttgctg cgctccagca cgcgaatatt cggatggttc agcgccttgc
    15061 tcaccagcgt ggtttctact tctctaccag ttgcgtcggc ggcatgaaga atacgacggt
    15121 gactatgtcc accttcacgg gtcagatggt agctttcttc gccattcggc tgaatgtggg
    15181 tatcaaataa caccccctgg tcgattagcc attgcacaca ggatcgtgca ttgctggcga
    15241 caaattcaac tgcatggcga tcgcaaatac cagccccggc aatcaatgtg tcttccacat
    15301 gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat accgccctgg gcataaaatg
    15361 ttgaaccttc cgttaccggg cctttactta gaacgatgac ctgatgctgg tcagccaggc
    15421 gtagcgccag tgaaagcccg gctgcgccgc taccgataat caacacgtca catgaatgtt
    15481 cagggagagt attcattttc tttgtttaat ttactaaaca cggtttggtc agcatagcat
    15541 catgttgtgc gaataaacac ctgctatttt aatatttgtt acagtggcta aacacgctga
    15601 tgcagggcgg cgagaaaaag agaagttact ggctggtgga ggattaggtg gtgaaataaa
    15661 aaggccgttg ggttactctt caggcagtta aatgggcatt tctacacaga taatgcgatg
    15721 ttcagattct gtagacttat aatgatagat aatgatccgt ctacagcatg acaaacaaaa
    15781 acagatgcgt tacggaactt tacaaaaacg agacactcta atcctttgct tgctcaaatt
    15841 gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt ggtttgggga gactttacct
    15901 cggatgagcg agcagttaac ggaccaggtc ctggttgaac gggtccagaa gggagatcag
    15961 aaagccttta acttactggt agtgcgctat cagcataaag tggcgagtct ggtttcccgc
    16021 tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag cttttattaa agcctatcgt
    16081 gcgctggatt cgttccgggg agatagcgct ttttatacat ggctgtatcg gattgctgta
    16141 aatacagcga aaaattacct ggttgctcag gggcgtcgtc caccttccag tgatgtggat
    16201 gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga aagaaatttc gaaccctgag
    16261 aacttaatgt tgtcagaaga actgagacag atagttttcc gaactattga gtccctcccg
    16321 gaagatttac gcatggcaat aaccttgcgg gagctggatg gcctgagcta tgaagagata
    16381 gccgctatca tggattgtcc ggtaggtaca gtgcgttcac gtatcttccg agcgagggaa
    16441 gctattgata acaaagttca accgcttatc aggcgttgac gatagcggga tactggataa
    16501 gggtattagg catgcagaaa gaacaacttt ccgctttaat ggatggcgaa acgctggata
    16561 gtgagctgct taacgaactg gctcataatc cagaaatgca gaaaacctgg gaaagctatc
    16621 acttaatccg tgactcaatg cggggtgata ctcccgaggt gctccatttc gatatctctt
    16681 cacgcgtgat ggccgccatt gaagaagagc cagtacgtca accggcgaca ttgatcccgg
    16741 aagcccagcc tgcgccgcat caatggcaga aaatgccatt ctggcagaaa gtacgtccgt
    16801 gggcggcaca gcttacccaa atgggcgtag ccgcatgcgt atcgcttgca gttatcgttg
    16861 gcgtccagca ctataatgga caatctgaaa cgtcccagca gcccgaaacg ccggtattta
    16921 atacactgcc gatgatgggt aaagccagcc cggttagcct gggagtacct tctgaagcga
    16981 ccgcaaataa tggtcaacag cagcaggtac aggagcagcg tcgtcgcatt aatgcaatgt
    17041 tgcaggatta cgaactgcaa cgccgactcc actctgaaca gcttcagttt gagcaggcgc
    17101 aaacccagca agccgctgta caggtgccag gaattcaaac tttaggaacg caatcgcagt
    17161 aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt agcctgttat tctctgctaa
    17221 cgcctcggcc actcccgcgt ccggggcgtt attacagcag atgaacctgg ccagtcagtc
    17281 actgaattac gagctgtcat tcatcagcat caataaacag ggtgttgagt ctctgcgtta
    17341 tcgacatgca cgcctcgata accgtcctct tgcacaattg ttgcaaatgg atggcccgcg
    17401 ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt gaaccgggac ttgaaccgtt
    17461 cacgcttaat ggcgattaca ttgttgattc tctgccatcg cttatctata ccgatttcaa
    17521 acgcctttct ccttactacg actttatctc cgtcgggcgc acgcgtattg ctgatcgtct
    17581 ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc tacagctaca tcgtgtggat
    17641 ggacaccgaa tcgaaattac cgatgcgggt tgatctcctt gatcgcgatg gtgaaacgct
    17701 ggaacaattt cgcgtgattg cttttaacgt caatcaggat atcagcagca gtatgcagac
    17761 gctggcgaag gcaaatttgc cgccgttgct ttctgttcct gtaggtgaaa aagctaaatt
    17821 cagctggacg ccaacctggt tgccacaggg ttttagcgaa gtttccagta gtcgacgtcc
    17881 gctaccgacg atggacaaca tgcctatcga atcacgtctc tattccgacg gattattcag
    17941 cttctcggta aacgttaacc gcgctacgcc atcgagcacc gatcagatgt tgcgcaccgg
    18001 acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa atcaccattg tcggtgaact
    18061 gccgccgcaa acggcgaaac gcattgccga gaatattaag ttcggggcag cgcaatgatc
    18121 aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg cgctggtcag ttgtgatgtt
    18181 aaagcttcat gcagcagctg cgcttcacgc gccggttgcg gtagccgcgt gttaaataaa
    18241 cttggcccgc aaaccacgca taccattgtc gtaccctgtg atgagccgtt agtgccgggg
    18301 caaaaagtgg aattggggat cgccgaaggc agcctgctta gctccgcatt actggtttat
    18361 atgtcgccgc tggtgggatt attcctcatc gcttcgctat ttcagctact ctttgcttct
    18421 gacgtcgcag cattatgtgg tgcggtcctc ggcggtattg gcgggttcct gattgcgcgc
    18481 ggctactcgc gtaagtttgc tgcccgggca gaatggcaac cgatcatctt aagcgtggca
    18541 ttgccgcctg gccttgtgcg atttgaaaca tcttcggaag acgcgagcca gtgatttcac
    18601 tggctttgtt gcattttgca taacaaatgc gaggacgttt ccagatacca caatccatac
    18661 ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat gcggcgtttc tattaataca
    18721 gacgttaagc tcagaacagc gacttctaaa agcctggtta accgggcatt aaggcacaat
    18781 aatcatactt tatgaagaat atacgtaact tttcgatcat agctcacatt gaccacggta
    18841 aatcgacgct gtctgaccgt attatccaga tctgcggtgg cctgtctgac cgtgaaatgg
    18901 aggcgcaggt tctcgattcc atggatcttg agcgtgagcg tggcattacc atcaaagcgc
    18961 aaagcgtgac gctggactac aaagcgtctg acggcgaaac ctatcagctt aactttatcg
    19021 acaccccggg ccacgtagac ttctcctatg aagtttcccg ttcgctggct gcctgtgaag
    19081 gtgcattgtt ggtggtcgac gccgggcagg gcgtggaagc gcaaaccctg gcaaactgct
    19141 acaccgccat ggaaatggat ctcgaagtgg tgccagtact gaacaagatt gacctgccgg
    19201 cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat cgtcggcatc gacgccactg
    19261 acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca ggacgttctc gaacgtctgg
    19321 tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc gttgcaggca ctaattatcg
    19381 actcctggtt cgacaactac ctgggcgttg tttcacttat ccgtattaaa aacggcaccc
    19441 tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca gacctataac gccgaccgtc
    19501 tgggcatctt cacgccgaaa caggttgacc gcactgaact gaaatgtggc gaagtaggct
    19561 ggctggtatg tgcgattaaa gatatccacg gcgctccagt cggcgatacc ttaacgctgg
    19621 cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa agtcaaaccg caggtatacg
    19681 ccggtctgtt cccggtaagt tccgacgact atgaagcctt ccgtgacgcg ctgggtaaac
    19741 tcagcctgaa cgatgcctca ctgttctatg agccggaaag ctccagcgcg ctgggctttg
    19801 gtttccgctg cggcttcctt ggcctgctgc acatggagat catccaggag cgtctggaac
    19861 gtgaatacga tctggatctg atcaccactg cgccgaccgt agtgtatgaa gttgaaacca
    19921 cgtcaagaga agttatctac gtcgacagcc catccaagct gcctgcggta aataacattt
    19981 acgaactgcg cgagccgatt gcagaatgtc acatgctgct gccgcaggca tatctcggca
    20041 acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac caatatggtt taccacggta
    20101 atcaggtggc actgacgtac gagatcccga tggcggaagt ggtgctcgac ttcttcgatc
    20161 gactgaaatc tacctcgcga ggttatgcgt ctctggatta caacttcaaa cgcttccagg
    20221 cgtccgacat ggtacgtgta gacgtattaa tcaacggtga acgtgttgat gcgctggcgt
    20281 tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga gttggtggag aagatgaaag
    20341 atctgatccc acgccagcag tttgatatcg ccattcaggc agcgattggt acgcacatta
    20401 ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact ggctaaatgt tatggcggcg
    20461 atatcagccg taagaaaaag ctgctgcaga agcagaaaga aggtaagaaa cgcatgaagc
    20521 agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc cattctgcac gtcggcaaag
    20581 acaacaaata acccttagga gttggcatgg cgaatatgtt tgccctgatt ctggtgattg
    20641 ccacactggt gacgggcatt ttatggtgcg tggataaatt ctttttcgca cctaaacggc
    20701 gggaacgtca ggcagcggcg caggcggctg ccggtgactc actggataaa gcaacgttga
    20761 aaaaggttgc gccgaagcct ggctggctgg aaaccggtgc ttctgttttt ccggtactgg
    20821 ctatcgtatt gattgtgcgt tcgtttattt atgaaccgtt ccagatcccg tcaggttcga
    20881 tgatgccgac tctgttaatc ggtgatttta ttctggtaga gaagtttgct tatggcatta
    20941 aagatcctat ctaccagaaa acgctgatcg aaaccggtca tccgaaacgc ggcgatatcg
    21001 tggtctttaa atatccggaa gatccaaagc ttgattacat caagcgcgcg gtgggtttac
    21061 cgggcgataa agtcacttac gatccggtct caaaagagct gacgattcaa ccgggatgca
    21121 gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta ctcaaacgtg gaaccgagcg
    21181 atttcgttca gaccttctca cgccgtaatg gtggggaagc gaccagcgga ttctttgaag
    21241 tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc cgagcgtaaa gagacactgg
    21301 gtgatgtgac gcaccgcatt ctgacagtgc cgattgcgca ggatcaggtg gggatgtatt
    21361 accagcagcc agggcaacaa ctggcaacct ggattgttcc tccgggacaa tacttcatga
    21421 tgggcgacaa ccgcgacaac agcgcggaca gccgttactg gggttttgtg cctgaagcga
    21481 atctggtcgg tcgggcaacg gctatctgga tgagcttcga taagcaagaa ggcgaatggc
    21541 cgactggtgt gcgcttaagt cgcattggcg gcatccatta atagctcatc ttcgttcacg
    21601 ttgtcgccgt tatggcgaca acgtgaatta tttatgagat aaatctcccg tggctaacga
    21661 catcccccgt cgttgtgtat agaatattcc cccgaagttt aaggttggca cctccaggtt
    21721 gccacggcac acgaaacagc gttggtcccc atataccgct aaactgaaac tgcagcgaag
    21781 cagttagcag agccatgtat atcaggtctg tttcgtgtgc tgaattgttg acgcatttat
    21841 ttattggtat cgcatgaacc ccatcgtaat taatcggctt caacggaagc tgggctacac
    21901 ttttaatcat ccggaactgt tgcagcaggc attaactcat cgtagtgcca gcagcaaaca
    21961 taacgagcgt ttagaatttt taggcgactc tattctgagc tacgttatcg ccaatgcgct
    22021 ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc cggatgcgcg ccacgctggt
    22081 ccgtggcaat acgctggcgg aactggcgcg cgaatttgag ttaggcgagt gcttacgttt
    22141 aggaccaggt gaacttaaaa gcggtggatt tcgtcgtgag tcaattctcg ccgacaccgt
    22201 cgaagcatta attggtggcg tattcctcga cagtgatatt caaaccgtcg agaaattaat
    22261 cctcaactgg tatcaaactc gtctggacga aattagccca ggcgataaac aaaaagatcc
    22321 gaaaacgcgc ttgcaagaat atttgcaggg tcgtcatctg ccgctgccga cttatctggt
    22381 agttcaggtc cgtggcgaag cgcacgatca ggaatttaca atccactgcc aggtcagcgg
    22441 cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt aaggctgagc aggctgccgc
    22501 cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg ataaaagtta ctgcggattt
    22561 attgccatcg tcggacgtcc gaacgttggc aaatccacat tgttgaacaa actgctgggg
    22621 cagaaaatct ccatcacctc ccgcaaggcg cagacaactc gtcaccgcat tgtggggatc
    22681 catactgaag gcgcgtatca ggcgatctac gtcgatacac cgggcctgca tatggaagaa
    22741 aaacgcgcca ttaaccgcct gatgaacaaa gcggcgagca gctctattgg cgatgttgag
    22801 ctggtgattt ttgtcgttga aggcacccgc tggacgccgg acgacgaaat ggtgctcaac
    22861 aaactgcgcg aaggcaaagc gccggtaatc ctcgcggtga acaaagtgga caacgtgcag
    22921 gagaaagccg atctgctgcc gcacctgcag ttcctggcaa gccagatgaa cttcctcgat
    22981 atcgtgccaa tctctgccga aaccgggctg aatgtcgaca ctatcgcagc aatcgtgcgt
    23041 aagcatctac ctgaagcgac tcatcacttc ccggaagatt acatcaccga tcgctcacag
    23101 cgttttatgg cgtctgaaat catccgcgaa aaactgatgc gtttcctcgg cgctgaactg
    23161 ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta acgaacgcgg cggttatgac
    23221 atcaacggtt tgattctcgt tgagcgtgaa gggcagaaga agatggttat tggcaacaaa
    23281 ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag acatgcagga aatgtttgaa
    23341 gcgcctgttc accttgaact gtgggtaaaa gtgaaatccg gttgggccga cgacgaacgc
    23401 gcactgcgca gtctcggtta cgttgacgat ctttaagagt cactccgatg gaaggctggc
    23461 agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac cagcctgatg ctggacgtct
    23521 tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg cgcacgctct aaacgctcta
    23581 ctctgaaagg tgcattacag cctttcaccc ctctcttgct acgttttggc gggcgtggcg
    23641 aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc gctgccatta agcggtatca
    23701 cgctttacag cggtctgtac atcaacgaac ttctctcccg cgtactggaa tacgagacgc
    23761 gcttctctga actctttttc gattacttgc actgcattca gtctcttgca ggtgccactg
    23821 gtacgccaga acccgcgctg cgccgctttg aactggcact gctcgggcat ctgggctatg
    23881 gcgtcaattt tacccattgc gcgggtagcg gcgagccggt agatgacacc atgacgtatc
    23941 gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat cgacaataaa acgttcaccg
    24001 gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga cgcagacaca ctacgcgccg
    24061 cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg cggtaaacca ttaaagagca
    24121 gggaactgtt ccggcagttt atgcctaagc gaacggtgaa aacacattat gaatgatgag
    24181 gattgtcatg gctgaattac tgttaggcgt caacattgac catattgcaa cgctgcgtaa
    24241 cgcacgcggt actgcttacc cggatccggt gcaggcggcg tttattgccg agcaggcggg
    24301 agcggacggc attaccgtgc atttacgtga agatcgccgt cacattactg accgcgacgt
    24361 gcgcatcctg cgtcagacgc tggatacccg tatgaatctg gagatggcag tgaccgaaga
    24421 gatgctggcg atcgccgttg agacgaagcc acatttttgc tgcctggtac cggaaaagcg
    24481 tcaggaagta acaaccgaag gcggcctgga cgttgcaggg cagcgtgaaa aaatgcgcga
    24541 tgcctgcaaa cgtctggcag atgccgggat ccaggtttct ctctttatcg acgccgatga
    24601 agagcagatc aaagctgcgg cagaggttgg cgcaccgttt atcgagatcc acaccggttg
    24661 ctatgctgat gccaaaactg acgccgaaca ggcgcaagag ctggcgcgta tcgccaaagc
    24721 cgcgaccttt gccgctagtc tcggtctgaa agttaacgct ggtcacggtc tgacctatca
    24781 caacgtgaaa gctattgccg ccatccctga gatgcatgaa ctgaatatcg gtcatgccat
    24841 tattggtcgt gcagtgatga ccggactgaa agatgcggtg gcagaaatga agcgtctgat
    24901 gctggaagcg cgtggctaat ggcaatatta ggtttaggca cggatatcgt agagatcgct
    24961 cgcatagaag cagtgatcgc ccgatccggt gaacgcctgg cacgccgcgt attaagcgat
    25021 aacgaatggg caatctggaa aacgcaccac cagccggtgc gttttctggc gaagcgtttt
    25081 gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga tccgcaatgg tctggcgttt
    25141 aatcaatttg aagtattcaa tgatgagctc ggcaaaccac ggctacggct atggggtgag
    25201 gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc atgtaacgct ggcagatgag
    25261 cggcactatg cttgtgccac ggtaattatt gaaagttaaa aataataccg ttctctttaa
    25321 aaagtaaaac ccccgaactg gtaaggaacg ggggttttac tttttaactt taattttgtg
    25381 cagcatgcac cctcaacaac agcgattatg gatcagtgta cttatctttt acaagcaact
    25441 tgcaatcttt agcataaaaa ctcgagcctt tacgaagaaa gcaatattga tggaaagatt
    25501 aacgtgaccg ccaattcgta agtacattaa aattggcttc gttattgaag attttgctgt
    25561 gctttacacc atgccacaga attcccccat tgaaacgagt ggtgtcgtca aagctctggt
    25621 gtggagtgca gcatgcaccc tcaataactc gcacgttcag ttttggggag atgtaagggc
    25681 taatctgaat ggctgcattc cttgtttaag gaaaaacgaa tgactgattg ccgatacctg
    25741 attaaacggg tcatcaaaat catcattgct gttttacagc tgatccttct gttcttataa
    25801 cacaaggaaa cgtacttaag gtgcgtccgg tgaaccagtc ggacgcacct ttaataacta
    25861 taaataagtg tctgggcaga tactatataa attaacttag tgaatgatta tgctaatgtc
    25921 atcaattaaa taaatataat ggcgttaagg cttcccagta atataattaa tactctactt
    25981 ccagagtaga atattaaatt ttatccgcgt ggtgcatcag cacaaattta tcccacaact
    26041 gttcttctgt ctcgacatgc gccggatctt tcacaatagt attggggatc gggcacacct
    26101 tctggcaggt tggtgtctcg tagtgcccta cgcattcggt acacttatcg ctgttaatct
    26161 cgtagatatg atctcccatt gaaatcgcct cattcgggca ttcgggttca cacatatcac
    26221 aattgatgca ttttttagtg attaacaacg ccataaggaa actctcaaaa caacacaaac
    26281 cgggcgggca ttatacgcgc attctttact cagaccagtt ttttcaccag cgcttcacta
    26341 tgacgaatac gttctggtgc cagttccaga tcctgctgaa tcagcgcaat gaacagcaaa
    26401 tccgttaaca ttccctgagc gtgacaagca gagattgaag cactgtttgt cgcttgttct
    26461 tcggcaatgg tatacaggca atgagaagca cgctgttgca gggcattcgg agtaaagccg
    26521 gtaatcgcca gcacttttcc gcccactcgc agcatctcat ctgccgctag gtttaactcg
    26581 cgtcgtacac cggtgtagga aatggctaat aacagatcgt caggggacga cgcctgtact
    26641 gttgcgagta gcgcatgcat atcgcgcact gcggcagcat tgaagccaat cttcatcagc
    26701 ttccaggcaa agttttgcgc caccagaccc gaagcgccaa taccggtcag aattatccgc
    26761 cgcgcagagc gcaacattgt tacgcattca tgcagtttct cttcactatt aacgtttagc
    26821 gttgcgtaca tagcggcagt attttcttta atcagttttt cgccgaccag ccgtaacgga
    26881 tcatcaccgc ggatttggtt atgaatgggc acggagggtg attccggctg gcttgccagc
    26941 gcttcactca acgccaactt aagcgccgga aaacctttat agccgagttt ttgcgcgaac
    27001 ttcacgacgc tggactgact gactccggct tcgttggcca gttgctgaga gcttaaatgg
    27061 cgcgccgtat caggttgtag cagcagataa tccgccagtt ttttatcact ctgggcaagc
    27121 ccctggtaac gctgacggat acgaagtaag ccgttcatat ctttcctcgc gatgaaatag
    27181 tggcgacaac aggcaatatg agacacacgc gagtgaattt tatattccat tgccgctaat
    27241 tgccatgaat taaaaattcc agttagccat gatgcggtcg attgttgccg gaaaagtgat
    27301 gatctatctg ttaggctatg tagtagcgaa aattaatcag gatgtttcag tccagaggag
    27361 tatggtttgg caactcacga gcgtcgtgtg gtgttttttg acttagatgg aacattgcat
    27421 cagcaggata tgttcggcag ttttctgcgc tatttactac gtcgccaacc gctgaatgcg
    27481 ttacttgtcc tgccgttgtt accgattata gccattgcgt tattgataaa aggtcgtgcg
    27541 gcacgctggc cgatgagtct gcttctgtgg gggtgcactt ttggtcacag cgaagcacgt
    27601 ttacagacgt tgcaggccga tttcgtgcgc tggtttcgcg acaatgttac cgcctttccg
    27661 ctggttcagg agcgattaac cacctacctg ttaagttccg atgctgatat ctggttgatt
    27721 accggctctc cgcagccgct ggttgaagcg gtttatttcg atacgccctg gctgccgcgg
    27781 gttaatctta tcgccagcca aattcagcgt ggctatggtg gttgggtatt gacgatgcgt
    27841 tgtctgggac atgaaaaggt cgcacaactg gagcgcaaaa tcggcactcc gctgcggctg
    27901 tacagtggct atagcgacag taatcaggac aatccgctgc tttatttctg tcagcatcgt
    27961 tggcgagtaa ccccgcgcgg tgaactccag caactggaat agagtaaagc atagcgtccg
    28021 tgtataatgc gccgcgcttt tataaccgga gttttctttt tgtctgaagt cgaatttagc
    28081 cacgaatact ggatgcgtca cgcgctgacg ctggcgaaac gtgcctggga tgagcgggaa
    28141 gtgccggtcg gcgcggtatt agtgcataac aatcgggtaa tcggcgaagg ctggaaccgc
    28201 ccgattggtc gccatgatcc caccgcacat gcagaaatca tggccctgcg gcagggtggt
    28261 ctggtgatgc aaaattatcg tctgatcgac gccacgttgt atgtcacgct tgaaccatgt
    28321 gtaatgtgtg ccggagcgat gatccacagt cgcattggtc gcgtggtctt tggtgcgcgt
    28381 gacgcgaaaa ctggcgctgc gggatcttta atggatgtgc tgcatcatcc gggtatgaat
    28441 caccgagtgg aaattacgga aggaatactg gcggatgagt gcgcggcgtt gctcagtgac
    28501 ttctttcgca tgcgccgcca ggaaattaaa gcgcagaaaa aagcgcaatc ctcgacggat
    28561 taattttgtt tctcttcact ccctttcctg gaaaacagca gagagggaga atgggtcaaa
    28621 tagtttgatg acgactggga aagaaacgag agaaaaggaa atttctcttt gcccaactcc
    28681 gtaggcgata ccgccggata atcctgcgcc agttgcatcg ccgcttctgt agcctgcttc
    28741 tctttctctt gcagataacc caccaggcta atctgatact tacgaatatt ttcgacataa
    28801 gcgtaggctt catgtccacg agcgtagccg taagtcagct tgctgtaata gggtttctgg
    28861 ctaagtaaag gcagacgctg ttttacgtca gcccaactgt caggattccc tttggttttt
    28921 gccgtcaggg cgcgggcatc cagcatatgc gcatagccca tattgtacgc agcgagggca
    28981 aaccagatcc gctcgttctc cggcacactt tccggcactt tactcatcat atcctgcaaa
    29041 taacgcacgc caccgctgat gctctgttcg gcatcggtac gatccgtaat gccgaggctt
    29101 tgcgcggtat ttttggttaa catcatcatg ccgcgcacac ccgtcggtga agtggcctgt
    29161 gcatcccagt gcgattcctg ataagcaata gcggccagca aacgccagtc aatttcttcg
    29221 gcgtatttct caaacagggg ctttaactgc ggcagtaccg catcgacggc gcgtaaaaat
    29281 gtgcgcgtat cgacgtaatc aaaatcatcg ccatgcccca ggtatttctc ttcaatgcgt
    29341 gccagcgtac cgtcttcatt catttcgttg aagaagtcga gcagggcggc ggaaagggta
    29401 ttatcgccat ctaacgggct aaaccaagtc accggttgtt catcggtgat atcgagcgct
    29461 acggcgagct ccgggtgaac gcgctgaaac aggctgatgg cgacagaatc agcaatggtg
    29521 taatcgagtt ttccttcgat gacatcttcc attaattccg cagagccttt tttgtcgtct
    29581 accttccagc ttaattccgg gaattttgtt tctttcaggg tctggagatc gttaaccacc
    29641 acatgacccg gtgcaacggt gagttgctcc gccgtcaggt tgcccagcgt acgtgggcga
    29701 tactgaccca ctttataaac cagttgttgt gacacggaat aataggtagg gccaggctga
    29761 taatttttta cccgctcact gttatagaca agtcctgccg ccagcaggtc ggcgttacca
    29821 ttatcaaggt cgtcaaacag ctggctgata ttctgccgca cggtcacttt cagttttacg
    29881 ccgaggtaat cggcaaactg tttcgccagt tcgtaatcca ggccaaaagg tttcccgttg
    29941 atttcgttat aagtcagggg agtatgaatg gtgctcacac gcaactctcc ccgcgcttga
    30001 atggcggcga tacggttgtc ggctttacca aaccagggaa tggatggcca gagagcgacc
    30061 gcgagcagca gtgccagaat gccgatgaac agataattaa tctttaattt tttcaattag
    30121 ttaattctct gtgtcgtgcg cgtcccagct tgaaaaaacg taataatagt gaaaggttta
    30181 ctcataaatg agcggcattt tgcgtaaacc tgcgccagat ggcaacttat tacagccatt
    30241 ggcggcacgc gttgctaatt cacgatggtg attttatttc cacgcaaacg gtttcgtcag
    30301 cgcatcagat tctttataat gacgcccgtt tccccccctt gggtacaccg aaagcttaga
    30361 agacgagaga cttatgatgg aaattctgcg tggttcgcct gcactgtcgg cattccgaat
    30421 caacaaactg ctggcacgtt ttcaggctgc caggctcccg gttcacaata tttacgccga
    30481 gtatgtccat tttgctgacc tcaatgcgcc gttaaacgat gatgagcacg cacaacttga
    30541 acgcctgctg aaatatggcc cggcactcgc cagccacgcc ccgcaaggca aactcctgct
    30601 ggtgaccccg cgtcctggca ccatctctcc ctggtcttcg aaagcgaccg atattgccca
    30661 taactgcggg ctacaacagg taaaccgcct tgagcgcggc gttgcttact atatagaagc
    30721 cggtacgctg accaatgaac aatggcagca ggttaccgct gaactgcacg accgcatgat
    30781 ggaaacggtc ttttttgctt tagatgatgc agagcagttg tttgcccacc atcaaccgac
    30841 tccggttacc agcgttgatt tgctggggca gggccgtcag gcgctgatcg acgctaacct
    30901 gcgtcttggc ctggctctgg cggaagatga aattgactat ctgcaggatg ctttcacaaa
    30961 gcttggtcgt aacccgaacg acatcgagct gtatatgttc gctcaggcga actctgagca
    31021 ctgccgccac aaaattttta atgccgactg gattatcgac ggtgaacagc agccgaaatc
    31081 gctgttcaag atgatcaaaa atactttcga aaccacgcca gatcacgttc tctctgctta
    31141 taaagataac gccgccgtaa tggaaggttc tgaagtgggc cgctactttg ctgaccacga
    31201 aacgggccgc tacgatttcc atcaggaacc ggcgcatatt ctgatgaaag tcgaaactca
    31261 caaccacccg acggcgattt ctccgtggcc gggcgcggcg acgggttccg gcggcgaaat
    31321 tcgtgatgaa ggcgccaccg gacgcggtgc gaagccgaaa gcgggtttgg ttggtttctc
    31381 cgtttccaac ctgcgaattc ctggcttcga acagccgtgg gaagaagatt tcggtaagcc
    31441 tgagcgcatt gtcaccgcgc tggacatcat gaccgaaggc ccgctgggcg gcgcggcgtt
    31501 taacaacgaa tttggtcgtc cggcactgaa tggctacttc cgtacttatg aagaaaaagt
    31561 gaacagccac aacggcgaag agctgcgcgg ttatcacaaa ccgatcatgc tggcgggcgg
    31621 gatcggcaac attcgcgccg atcacgtaca aaaaggcgag atcaacgtcg gtgcgaagct
    31681 ggtcgttctc ggcggcccgg caatgaacat cggtcttggc ggcggagcgg cgtcttccat
    31741 ggcgtcaggt cagtctgatg ccgacctcga ctttgcttcc gtacagcgcg acaacccgga
    31801 gatggagcgt cgttgccagg aagtaatcga ccgttgctgg cagcttggtg atgctaaccc
    31861 aattctgttt atccacgacg tcggtgcggg cggtctttct aacgccatgc cggaactggt
    31921 gagcgacggc gggcgcggcg gtaaattcga actgcgcgat atcctgagcg acgaaccggg
    31981 catgagcccg ctggaaatct ggtgtaacga atcccaggaa cgctacgtgc tggcggttgc
    32041 tgctgatcaa ttaccgctgt ttgacgagct gtgtaagcgt gagcgcgcac cctacgctgt
    32101 gattggtgaa gcgaccgaag aactgcatct ttctctgcac gatcgtcatt ttgataatca
    32161 gccgatcgat ctgccgctgg acgtcctgct tggtaaaacg ccgaagatga cccgcgatgt
    32221 acaaacgctg aaagcgaaag gcgacgcgct ggcccgtgaa gggatcacca ttgcagacgc
    32281 ggtgaaacgt gtgctgcatc tgccgactgt ggcggaaaaa accttcctgg tgaccattgg
    32341 cgaccgcagc gtaaccggca tggtagcgcg cgatcagatg gtggggccgt ggcaggtgcc
    32401 ggtcgctaac tgcgcggtca ctaccgccag cctcgacagc tactacggtg aagcgatggc
    32461 gattggcgag cgtgcgccgg ttgcgctgct ggatttcgcc gcctctgccc gtctggcggt
    32521 cggtgaagcg ttaaccaaca tcgccgcaac acaaattggc gatatcaaac gcatcaaact
    32581 ttccgccaac tggatggcgg cggcaggcca ccctggtgaa gatgcgggcc tgtatgaagc
    32641 cgttaaagcc gtgggcgaag agctttgtcc ggcgctgggc ctgacgatcc cggtgggtaa
    32701 agactccatg tcgatgaaaa cccgctggca ggaaggtaac gaagagcgcg aaatgacgtc
    32761 gccgctgtcg ctggtgattt ctgcatttgc ccgcgtggaa gatgtacgtc acaccatcac
    32821 gccgcagctt tctaccgaag ataacgcact gctgctgatt gatttgggca aaggcaataa
    32881 cgcgctgggc gcaacggcgc tggcgcaggt ttatcgtcag cttggcgaca aaccggcaga
    32941 tgtacgcgat gtcgcgcaac tgaaaggctt ctatgacgcg attcaggcgc tggttgcaca
    33001 gcgtaagctg ctggcgtatc acgaccgctc tgatggcggc ctgctggtaa cgctggcgga
    33061 aatggccttt gctggtcatt gtggcattga cgcggatatc gccactctgg gtgacgatcg
    33121 cctggcggcg ttgtttaacg aagaactggg tgcggtgatt caggttcgtg ccgctgaccg
    33181 tgaagcggtc gagtccgtac tggcacagca tgggcttgcg gattgcgtcc attatgttgg
    33241 gcaggcggtt tccggtgacc gttttgtgat taccgccaac gggcagactg tattcagcga
    33301 aagccgcact acgttgcgtg tctggtgggc agaaactacc tggcagatgc agcgcctgcg
    33361 tgacaacccg gagtgtgccg atcaggagca tcaggcgaaa tcaaacgacg ccgatccggg
    33421 cctgaatgta aaactgtcgt tcgatatcaa cgaagatgtg gcagcaccgt atattgccac
    33481 tggcgcacgt ccgaaagttg ccgtgctgcg tgagcagggt gtgaactcac atgttgaaat
    33541 ggcggcagcc ttccaccgtg caggcttcga tgcaatcgat gtgcatatga gcgacctgct
    33601 ggctggacgc acgggcctga aggattttca cgccctggtc gcgtgcggag gtttctccta
    33661 cggtgacgtg ctgggtgccg gtgaaggttg ggcgaagtct atcctgttca atgaccgtgt
    33721 acgcgatgag tttgcaacct tcttccatcg tccgcaaacg ctggcgctgg gggtatgtaa
    33781 cggttgccag atgatgtcta atctgcgtga actgatccca ggtagtgagt tgtggccacg
    33841 ttttgtgcgc aatacctccg atcgctttga agcgcgtttc agcctggttg aagtgaccca
    33901 aagcccgtcg ctgctgttgc aggggatggt gggctcgcaa atgccgattg ctgtctctca
    33961 tggtgaaggg cgcgtggaag tgcgtgatgc ggcgcatctg gcggcactgg aaagcaaagg
    34021 gctggtggca ctgcgctatg tcgataactt cggcaaagtc actgaaacct acccggctaa
    34081 cccgaacggt tcaccgaacg gtattacggc agtcacgact gaaagtggtc gagtcaccat
    34141 tatgatgccg cacccggaac gtgttttccg tactgtcagc aactcctggc atccggaaaa
    34201 ctggggcgag gatggcccat ggatgcgtat tttccgcaat gcgcgtaagc agttggggta
    34261 agtcgtcagc ccattggttt tgcagcccgg atgcggcatt aagca
//
LOCUS       DAOFQD010000045        33945 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.045, whole genome shotgun sequence.
ACCESSION   DAOFQD010000045 DAOFQD010000000
VERSION     DAOFQD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.045"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(135..1388)
                     /locus_tag="QBF46_002155"
     CDS             complement(135..1388)
                     /locus_tag="QBF46_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDQ6674335.1"
                     /translation="MALTDAKIRAAKPDEKPYKLADSGNMFLLVHPNGSRYWRLRYRF
                     LGKEKTLALGVYPEVSLSEAREKRDAARKQIAEGTDPCEQKRIKKSVPETAQTFEGIA
                     RQWHKSNKKWSASHSEKILKSLETHVFPFIGSRDITTLRTPDLLVPVKAAEAKEIYEI
                     AARLQQRITAIMRYAAQSGIITYNPAVDMAGALTTVKRQHRPALALNRISELLERLDT
                     YRGQPLTRLATKLTLLIFIRSSELRFARWSEIDFKKAMWTIPPERKPIEGVKYSHRGS
                     KMRTEHLVPLSTQALDILKQIQVISGEHELIFTGDHNPWKPMSENTVNNALRLMGYNT
                     KVDVCGHGFRAMACSSLIESGLWSRDAVERQMSHQERNGVRAAYIHKAEFLDERKLML
                     QWWADFLDANSENPVSPFDYARQQL"
     gene            complement(1584..1659)
                     /locus_tag="QBF46_002156"
     tRNA            complement(1584..1659)
                     /locus_tag="QBF46_002156"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1624..1626),aa:Phe,seq:gaa)
     gene            complement(1765..2472)
                     /locus_tag="QBF46_002157"
     CDS             complement(1765..2472)
                     /locus_tag="QBF46_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDQ6674336.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2870..5005
                     /gene="speC"
                     /locus_tag="QBF46_002158"
     CDS             2870..5005
                     /gene="speC"
                     /locus_tag="QBF46_002158"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDQ6674337.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPYR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLVE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5055..6311)
                     /gene="nupG"
                     /locus_tag="QBF46_002159"
     CDS             complement(5055..6311)
                     /gene="nupG"
                     /locus_tag="QBF46_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDQ6674338.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6513..7592)
                     /gene="mltC"
                     /locus_tag="QBF46_002160"
     CDS             complement(6513..7592)
                     /gene="mltC"
                     /locus_tag="QBF46_002160"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDQ6674339.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7657..7932)
                     /locus_tag="QBF46_002161"
     CDS             complement(7657..7932)
                     /locus_tag="QBF46_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDQ6674340.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7960..9012)
                     /gene="mutY"
                     /locus_tag="QBF46_002162"
     CDS             complement(7960..9012)
                     /gene="mutY"
                     /locus_tag="QBF46_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDQ6674341.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVGLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9173..9892
                     /gene="trmB"
                     /locus_tag="QBF46_002163"
     CDS             9173..9892
                     /gene="trmB"
                     /locus_tag="QBF46_002163"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDQ6674342.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9892..10218
                     /locus_tag="QBF46_002164"
     CDS             9892..10218
                     /locus_tag="QBF46_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDQ6674343.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            10402..11121
                     /locus_tag="QBF46_002165"
     CDS             10402..11121
                     /locus_tag="QBF46_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDQ6674344.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11297..12343
                     /gene="ansB"
                     /locus_tag="QBF46_002166"
     CDS             11297..12343
                     /gene="ansB"
                     /locus_tag="QBF46_002166"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDQ6674345.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12460..13467
                     /locus_tag="QBF46_002167"
     CDS             12460..13467
                     /locus_tag="QBF46_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDQ6674346.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13622..14758)
                     /gene="hemW"
                     /locus_tag="QBF46_002168"
     CDS             complement(13622..14758)
                     /gene="hemW"
                     /locus_tag="QBF46_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDQ6674347.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14751..15344)
                     /locus_tag="QBF46_002169"
     CDS             complement(14751..15344)
                     /locus_tag="QBF46_002169"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDQ6674348.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15352..15642)
                     /locus_tag="QBF46_002170"
     CDS             complement(15352..15642)
                     /locus_tag="QBF46_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDQ6674349.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(15639..16205)
                     /gene="yggT"
                     /locus_tag="QBF46_002171"
     CDS             complement(15639..16205)
                     /gene="yggT"
                     /locus_tag="QBF46_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDQ6674350.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16223..16927)
                     /gene="yggS"
                     /locus_tag="QBF46_002172"
     CDS             complement(16223..16927)
                     /gene="yggS"
                     /locus_tag="QBF46_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDQ6674351.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16945..17925
                     /locus_tag="QBF46_002173"
     CDS             16945..17925
                     /locus_tag="QBF46_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDQ6674352.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHRVGEGRL"
     gene            complement(18109..18525)
                     /gene="ruvX"
                     /locus_tag="QBF46_002174"
     CDS             complement(18109..18525)
                     /gene="ruvX"
                     /locus_tag="QBF46_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDQ6674353.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(18525..19088)
                     /locus_tag="QBF46_002175"
     CDS             complement(18525..19088)
                     /locus_tag="QBF46_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDQ6674354.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19197..20147)
                     /gene="gshB"
                     /locus_tag="QBF46_002176"
     CDS             complement(19197..20147)
                     /gene="gshB"
                     /locus_tag="QBF46_002176"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDQ6674355.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(20160..20891)
                     /gene="rsmE"
                     /locus_tag="QBF46_002177"
     CDS             complement(20160..20891)
                     /gene="rsmE"
                     /locus_tag="QBF46_002177"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDQ6674356.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMVLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(20971..21678)
                     /gene="endA"
                     /locus_tag="QBF46_002178"
     CDS             complement(20971..21678)
                     /gene="endA"
                     /locus_tag="QBF46_002178"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDQ6674357.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RQNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21773..22270)
                     /locus_tag="QBF46_002179"
     CDS             complement(21773..22270)
                     /locus_tag="QBF46_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDQ6674358.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22347..23741)
                     /gene="galP"
                     /locus_tag="QBF46_002180"
     CDS             complement(22347..23741)
                     /gene="galP"
                     /locus_tag="QBF46_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDQ6674359.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(24166..25494)
                     /locus_tag="QBF46_002181"
     CDS             complement(24166..25494)
                     /locus_tag="QBF46_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDQ6674360.1"
                     /translation="MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTL
                     RKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSE
                     AVRRVFTKTAQLWHNATPHPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
                     YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLL
                     NALSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARL
                     LTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSK
                     KPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGAS
                     PGRIPELMHDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(25617..26771)
                     /gene="metK"
                     /locus_tag="QBF46_002182"
     CDS             complement(25617..26771)
                     /gene="metK"
                     /locus_tag="QBF46_002182"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDQ6674361.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(27075..27290)
                     /locus_tag="QBF46_002183"
     CDS             complement(27075..27290)
                     /locus_tag="QBF46_002183"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674362.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            27426..27557
                     /gene="yqgB"
                     /locus_tag="QBF46_002184"
     CDS             27426..27557
                     /gene="yqgB"
                     /locus_tag="QBF46_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDQ6674363.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            27566..29542
                     /gene="speA"
                     /locus_tag="QBF46_002185"
     CDS             27566..29542
                     /gene="speA"
                     /locus_tag="QBF46_002185"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDQ6674364.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGIFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            29679..30599
                     /gene="speB"
                     /locus_tag="QBF46_002186"
     CDS             29679..30599
                     /gene="speB"
                     /locus_tag="QBF46_002186"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDQ6674365.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(30805..31563)
                     /gene="loiP"
                     /locus_tag="QBF46_002187"
                     /gene_synonym="yggG"
     CDS             complement(30805..31563)
                     /gene="loiP"
                     /locus_tag="QBF46_002187"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDQ6674366.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            31841..33832
                     /gene="tkt"
                     /locus_tag="QBF46_002188"
     CDS             31841..33832
                     /gene="tkt"
                     /locus_tag="QBF46_002188"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDQ6674367.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPLNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
BASE COUNT         8157 a         8609 c         8980 g         8199 t
ORIGIN      
        1 gtaatcccct gtacctacgt aaaatagttc atcacaagga caggcggcag cttgccatca
       61 ccccctctgt gaataaatgt atacagggaa agaataagta aacattctcc cggcatttct
      121 gccgggagaa tagattataa ttgttgtctg gcgtaatcga aaggtgagac cggattttca
      181 ctattcgcat cgagaaaatc tgcccaccat tgcagcatca atttgcgttc gtctaagaac
      241 tcagctttat gaatgtaggc cgcacgtacg ccattacgct cctgatggct catctgacgt
      301 tcaacagcat ccctggacca tagaccagat tcaatcaatg aactgcatgc cattgccctg
      361 aagccgtggc cacaaacgtc aacttttgta ttgtagccca tcaggcgtaa cgcgttattc
      421 accgtatttt cactcatagg tttccatgga ttgtggtcgc cagtgaaaat tagctcgtgc
      481 tcaccgctga tgacctggat ttgtttgaga atatccagtg cctgagttga gagtggaacc
      541 agatgctctg tacgcatttt tgagccgcga tgggaatact tgaccccttc aataggtttt
      601 cgctcaggag gaatggtcca catggctttc ttaaagtcga tctcagacca cctggcaaag
      661 cgaagttcac tggaacggat gaagatgagc agtgtaagtt tagttgccag tcgggtgaga
      721 ggttgacctc tgtaggtatc cagtcgttca agtagttcag aaatgcgatt taaggcgagg
      781 gcagggcggt gctgacgttt caccgtggtt aacgcgccag ccatatccac ggcagggtta
      841 taggtgataa tgcctgactg ggcagcatag cgcatgattg cagtgatacg ctgctgaagg
      901 cgcgctgcaa tctcataaat ctctttggct tcggctgcct ttactggaac aagtaaatcg
      961 ggagttctga gggtggtaat gtcacgtgaa ccgataaagg gaaacacatg agtttctagg
     1021 cttttcagta tcttctcact atgtgaggct gaccattttt tgttgctctt atgccactgc
     1081 cttgcgatgc cttcaaatgt ctgagctgtt tcgggtaccg actttttgat tcgtttctgt
     1141 tcgcacgggt cagtaccttc agctatctgc tttctcgccg catcgcgttt ttcccttgct
     1201 tctgataacg agacttcagg atagacaccc agggcgagcg ttttctcttt gcccagaaac
     1261 cggtagcgga gtcgccagta gcgcgagccg ttagggtgaa cgagcaaaaa catgttgccg
     1321 ctgtcggcaa gtttataagg tttttcatcc ggtttagctg cacggatttt agcgtcagtg
     1381 agcgccattg gcggtatctc ctgatagtaa aaggggcata aattttatcg aatcagacta
     1441 taccctcagt tgtaccctcg caaggggctt gatgcgggta gaagttgatt gagctgggtg
     1501 gagttaatgg attgaagagg tgttgatttc tgcgggatta gaatgcaaaa acggacctcc
     1561 gtggaggtcc gttgatatga atttggtgcc cggactcgga atcgaaccaa ggacacgggg
     1621 attttcaatc ccctgctcta ccgactgagc tatccgggca acggggcgca ttaaacctga
     1681 ttcgcctcgt ctcgtcaatc aaatttcttc aatttactgc agactgcaca atctatcatc
     1741 actttgccga tattgcacgc attgtcaggc aaaccaggcg gtccaggcgg cggaaagcgg
     1801 cattgccaga aggagtgctg gaagcatgtt gaccaccggg aacattttaa tgccacagat
     1861 gcgtaatccg gttgccagca gcaataaacc gcctacggcg ctgaagtctg ccatcatcga
     1921 cggtgtggtc agcggtaaaa tcagcgcggc agcccacgcc agcgttaact ggatgatcag
     1981 taatgggata ctaatcaccg ataccgcaat acccagcgag caggcgaaga tcatcgccgt
     2041 aaagaaatca agaaatgact tggcgattaa aatactcgga tcgccggtca tcccttcgtt
     2101 catcgccccg aagatcccgg tgccgctggc gcaaaacagg acaataatcg cgacataatt
     2161 ctgaataaaa gattcatgcg ctggcttctt acgtgagtga cgaaacagat tttgtgcttt
     2221 ggcgactgct gtattgacac ctttctccag caaacaaatt tcgccaatta gagcgccaag
     2281 tagcgtagct aaaaccatcg ctggaaggtt ggcacatttc accaccagta aaataccaat
     2341 ccccagcgat gccagaccaa aaattgatgt catggagacg cggatacgtt ccggtaagcg
     2401 ttggctgagc agtgcgccga gaacgccacc cagtaagact gcactagcgt tgataaaagg
     2461 gccgatgacc acaagagttc ctgttagctg tattgtttga ttttcttatt atgaactttt
     2521 tgccttgttg atggcttgtc tggtcgcaaa gtcctggctt gcacgcttta gcgaaaggtg
     2581 ccatgattgc gcgaattttc tcctctctgt acggagtttg cccgatgcac gccatctcct
     2641 tacattctct cgcttatcgc cgtttcgcgc gaaacgtttc cctttctatg ttactgctca
     2701 tgcggtgaag ttaacgcacg ctcactgcag gacaacagta aaatcagagc gtttctgctt
     2761 ttactgatgt ctggcggtcg gagctggtga ccagtttgac ccatatctca tggggcaggg
     2821 ttttccacct tgtccggtat tcttacttcc ccgaaacggg tttgcgctta tgaaatcaat
     2881 gaatattgcc gccagtagtg aactggtatc ccgactttct tctcatcgtc gcgtggtggc
     2941 gttgggagat actgatttta cggacgtcgc ggcagtcgtc attaccgctg cggatagtcg
     3001 cagtggcatt cttgcgttgc ttaagcgcac cggttttcat ctaccggtgt ttttgtattc
     3061 cgaacatgct gttgaattac ctgcgggcgt tacggcggta atcaacggca acgagcagca
     3121 gtggctggag ctggaatccg cagcctgtca gtatgaagag aatttgctgc caccgtttta
     3181 tgacacgctg acgcagtacg ttgagatggg caacagcacc tttgcttgcc ctggacatca
     3241 acatggtgcg ttttttaaaa agcatcctgc cggacgccat ttttacgatt tctttggtga
     3301 gaacgtcttt cgcgccgata tgtgtaacgc tgacgtaaaa ttgggcgatc tgcttattca
     3361 tgaaggatcg gcgaaagatg cgcagaaatt cgcagccaaa gtctttcatg ccgataaaac
     3421 ctattttgtg ctgaacggca catcggcagc gaataaagtg gtgacgaatg cgctgttaac
     3481 gcgtggcgat ctggtgctct tcgaccgtaa caaccataag tcgaatcatc acggcgcgct
     3541 gattcaggcg ggggcgacgc cggtctatct ggaagcttca cgcaacccgt ttggtttcat
     3601 tggcggtatt gatgcgcact gttttaatga agagtatctg cgccagcaaa ttcgcgacgt
     3661 tgcgccagaa aaagccgacc tgccgcgccc gtatcgcctg gcgattattc agctgggaac
     3721 ctatgacggc actgtctata acgcccgtca ggtgatcgat accgttgggc atctgtgtga
     3781 ttacattctg tttgattccg cgtgggtcgg ttatgaacaa tttatcccga tgatggcgga
     3841 tagctcgccg ctgctgttag aacttaacga aaacgatccg gggatctttg tgactcagtc
     3901 ggtgcacaaa cagcaggcgg gattctcaca gacgtcgcag atccataaaa aagataacca
     3961 tatccgcgga caggcgcgtt tttgcccgca taagcggttg aataacgcct ttatgctcca
     4021 tgcttctacc agccctttct atccgctgtt tgctgcactg gatgttaacg ccaaaattca
     4081 tgaaggggag agtgggcgtc ggctgtgggc tgagtgtgtt gagataggga ttgaagcgcg
     4141 caaggctatt cttgcgcgct gtaagctgtt ccgcccgttt atcccgcccg ttgttgatgg
     4201 caaattgtgg caggattatc cgacatcagt gttagccagc gaccgccgtt ttttcagttt
     4261 tgagccgggg gcgaagtggc acggctttga aggatatgcc gcggatcagt attttgttga
     4321 tccgtgcaag ctgttactca ctacaccagg tatcgatgcc gaaaccggcg aatatagcga
     4381 ctttggcgtt ccggcgacga ttctggcgca ctatctgcgt gagaacggca ttgtgccgga
     4441 gaagtgcgat ctcaactcca ttctgttttt attaactccg gcggaaagcc acgagaagct
     4501 ggcacaactg gtggcgatgc tggcgcaatt tgaacagcat attgaggatg actcgccgct
     4561 ggttgaggtg ttgccgagcg tttataacaa gtatccggtg cgctatcgcg actacaccct
     4621 gcgccagttg tgtcaggaga tgcacgatct gtatgtcagt ttcgacgtca aagacctaca
     4681 aaaagcgatg ttccgccagc agagtttccc gtcagtggtg atgaaccccc aggatgcgca
     4741 tagcgcttat attcgcggtg acgtggagtt ggtgcggatt cgtgatgccg aagggcgaat
     4801 tgcggcagaa ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt
     4861 ctggggtggg gcggttcaac gttatttcct tgcactggaa gaaggggtga atttgttgcc
     4921 gggattttcg ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg
     4981 gttgtacggt tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt
     5041 ttttctttgc gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta
     5101 tatttgaaca tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac
     5161 catacggttt gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc
     5221 acgatgccgc cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca
     5281 ctggcgcgaa ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag
     5341 aagtcgaatg cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga
     5401 gtcgggtcgc cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc
     5461 attacgttct taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt
     5521 tcagagatct gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca
     5581 aacatcggat ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc
     5641 agttccgcgc ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac
     5701 agcgcgaatg catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca
     5761 accggaatat gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca
     5821 atatacagct gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg
     5881 ataaagccga tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg
     5941 gcattttgca ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca
     6001 aacgagttaa tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg
     6061 aacagcgtga tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat
     6121 ttgtcggcca caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta
     6181 taaactgcgc caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg
     6241 gtcgtcagcc aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc
     6301 ttaagattca tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt
     6361 tgaccgtgcg agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg
     6421 caaataattg caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg
     6481 cccctgagca tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg
     6541 gtattcactt tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc
     6601 tgataaacat cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc
     6661 gaaaagactc gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc
     6721 cgcgatgttg ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg
     6781 gtgccggtat caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat
     6841 ttcccctgcg agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc
     6901 agcgcatcgg aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc
     6961 agaatcagcg actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat
     7021 ttgtgcgcac gtttatcaag gtggttcggc accatgttaa tggtgacgct gtagatgata
     7081 cgcagcccgt tgctgcggct cttcagacgg tttttcagca gataatccgc gaagttgctt
     7141 gcgcgacctt cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa
     7201 ggttctttcg aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg
     7261 tcacccatca ataacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg
     7321 gcgatggttt cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat
     7381 tgatcggtgt atttcacgta gtccttagga ccagcgatca ccacctcttt gaagccccag
     7441 atgttctcaa tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg
     7501 acccaggctt cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc
     7561 gcaatcaaag ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt
     7621 tatgcaagtt gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata
     7681 gccctcgata tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag
     7741 cttgcggtgc tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt
     7801 gtgctgccac tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc
     7861 gccggggtac agctgaaaat cctgaccttc tgcttcacgt tgcaggaaag tacaaaaaat
     7921 cgttctgctc ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg
     7981 taactgctgt aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa
     8041 gttataccag agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca
     8101 cataggcaca atatctaagt ggaaatggct gaaggtatgg cgaaacgcgg tcagctgcgt
     8161 caggttatcg gcagcaatct gccgttgcgc cagccaatgc cgcaaacttt cttcgtcggc
     8221 aaactgcggg aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa
     8281 tacttcatct tcgtgctgta atagcaaaaa gtagccggtg cgctccggca gcgtctgttt
     8341 cggttttttg cccggataaa gcgaccagct attgttggcg gcggcaatac atccgttttg
     8401 tagcggacag agcgaacatt tcggtttcga gcgcgtacaa atcatcgcgc ccaaatccat
     8461 catcgcctga ttaaaccgtt ccacgccaac cgcgggcgtc acctgctcgc tcaaactcca
     8521 taatttattc tcgacctctt ttttccctgg ccagccgctt acagcatagc agcgcgccag
     8581 cacccgtttg acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat
     8641 cgcgcctgcg gtggaacgcc cgacgcccgg cagtgctgca acttcctcaa aggtttccgg
     8701 gaatttaccg ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg
     8761 ggcgtaatag ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag
     8821 atcggtcacc gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac
     8881 ctgagtttgt tgcaacatca cttctgagag ccctactttg tagggcgtct tgtcaatttg
     8941 ccagggcaga gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg
     9001 cgacgcttgc atggtcaccg aattcactgt tgttggaggc aagattgcag cacagcgacg
     9061 gcagggtgta aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact
     9121 aactttggat aatgcccgtt ttcagaacac tttcacaagc gactaaacct ttatgaaaaa
     9181 cgacgtcatt tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt
     9241 tgtgcgccgc caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc
     9301 ggtgatgggc gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga
     9361 agcgccggtg acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa
     9421 agatcgccct gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg
     9481 cctggcttct gcgcatgaag aaggtttaag caacctgcgc gtgatgtgtc acgatgcggt
     9541 tgaagtgctg cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc
     9601 tgacccgtgg cacaaagcgc gccataataa acgccgtatc gttcaggtgc cgtttgccga
     9661 actggtaaaa agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc
     9721 ttatgcggaa catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga
     9781 gagcaatgat tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg
     9841 tcatcgtctt ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag
     9901 aaccgtagcc gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt
     9961 tcggtggcat ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat
    10021 gattttatta acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg
    10081 gcctgggaag gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg
    10141 attgtgcgta agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc
    10201 gacgtttggt gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg
    10261 ctacgcttat caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc
    10321 gcatccggca tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg
    10381 attttttctt gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg
    10441 acggcaatga ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt
    10501 gtcagcccgc aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac
    10561 ggcaacgtga tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg
    10621 aaggattatc aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc
    10681 cgcgtcgaga aagcccgtat tgctctggat aaaattatcg ttcaggagat gggcgaaagc
    10741 agcaaaatgc gcagccgtct gaccaaactt gatgcgcagc tgaaagagca gatgaaccgc
    10801 attattgaaa cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc
    10861 gccgaaggcc agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat
    10921 gaaatgggcg cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga
    10981 agcctggggg ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc
    11041 cagcagtttg gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc
    11101 ctggtcggga atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt
    11161 ttaattcttc gttttgttac ctgcctctaa ctttgtagat ctccaaaata tattcacgtt
    11221 gtaaattgtt taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg
    11281 taactggagg aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg
    11341 ttttagtggt gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat
    11401 tgccggtggt ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga
    11461 aaatctggtt aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt
    11521 agtgaatatc ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat
    11581 taacaccgac tgcgataaga ccgacggctt cgtcattacc cacggtaccg acacgatgga
    11641 agaaactgct tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg
    11701 cgcaatgcgt ccgtccacgt ctatgagcgc agacggtcca ttcaacctgt ataacgcggt
    11761 agtgaccgca gctgataaag cctccgccaa ccgtggcgtg ctggtagtga tgaatgacac
    11821 cgtgcttgat ggccgtgacg tcaccaaaac caacaccacc gacgtagcga ccttcaagtc
    11881 tgttaactac ggtcctctgg gttacattca caacggtaag attgactacc agcgtacccc
    11941 ggcacgtaag cataccagcg acacgccatt cgatgtctct aagctgaatg aactgccgaa
    12001 agtcggcatt gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga
    12061 tgcgggctat gatggcatcg ttagcgctgg tgtgggtaac ggcaacctgt ataaatctgt
    12121 gttcgacacg ctggcgaccg ccgcgaaaaa cggtactgca gtcgtgcgtt cttcccgcgt
    12181 accgacgggc gctaccactc aggatgccga agtggatgat gcgaaatacg gcttcgtcgc
    12241 ctctggcacg ctgaacccgc aaaaagcgcg cgttctgctg caactggctc tgacgcaaac
    12301 caaagatccg cagcagatcc agcagatctt caatcagtac taatcgcctc gccccggtat
    12361 cgtgccgggg ctttttcact tcagactcac gtccattgcc aattttaatt accctaatga
    12421 taatcaccgg aataaattat tctgcgcgag ggttttcggg tgaaaaagca atggattgtt
    12481 ggcacggcgc tgcttatgtt gatgacaggt aatgtctggg cagatggcga accaccaact
    12541 gaaaatatct taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgct
    12601 atcaccttaa aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat
    12661 gtctcttcca gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc
    12721 gaacagacct ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga
    12781 acgcccgcgt ctggctggtc ggtgaacttt tactcttttc aggcggcagc cagcgatcgt
    12841 gggcgggtgg tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat
    12901 ttcaattatc gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct
    12961 gcgctggaaa aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg
    13021 gatgcatatt ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga cgcctttaaa
    13081 aaaattcacc aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt
    13141 aaatatgaca aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag
    13201 tgttatgaag tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag
    13261 acttttctgc aatcacaaaa acttagccgt aaattgcagg atgactgggt aacgctcgaa
    13321 aaagggcaat atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt
    13381 ttgatgaaaa ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa
    13441 ctgcggcgca atggggtgat taagtaagtt tgatatagtg cacgatttgt agcaggatgc
    13501 ggcgttgacc ccgcatccgg caatcaacgt ctgatgcgac gcttgcgcgt cttatcagac
    13561 ctacaaccgc tgccacatgt cggctggata aggcgttcac gccgcatccg gcaatacaag
    13621 tttactcagc cagaaaaagc tccagcagcg aatttaaaaa cagcttccca tgttccgtta
    13681 tctgccagta atccgcacat tcggtgagat aaccctgggc aatcgcctcg tctaactgtg
    13741 ggcgaatcac atcttcgcaa agcccggtat acgcaataaa ctccacgcgc ggcgcggcct
    13801 ccagcagacg gaagcgattc ataaagaact caaacggctt atctgcggct tcgacatcac
    13861 gctggctttc cagatacctt ccttgcataa aaccacgcgg atgacgcgtt ttagtggtac
    13921 gcagaatgcg cccatccggg aaggtcactt tgccgtgcgc gccgcagcca ataccgatgt
    13981 agtcgccaaa gcgccagtag ttgagattgt gctggcactg ataaccgggt ttggcgtaag
    14041 cggaagtttc atattgctga taacccgctg cggttaataa ctgatgcccc tgttcgaata
    14101 tatcccacag cgcgtcgtcg tccggcagta ccggcgggcg cgagccaaac agcgtattgg
    14161 gttcgatggt cagttgatac caggaaagat gcggcggatt cagttcaatg gcctggcgta
    14221 gatcgccaag cgcctcttcc agtgattgat ccggtagtcc atgcatcaaa tcaaggttaa
    14281 agctacgtaa ccctaaaccg cttgccagct ttgccgctcg tttcgcttct tgcgggccat
    14341 gaatacgccc aagtcgtttc agcttttctt cgctaaaact ctgcacacca atagagatgc
    14401 ggttcacacc agcacgctga taatcgacaa agcgatcggc ttctaccgtt ccagggttcg
    14461 cttccatagt aatttctgca tccgctgcca gcggcaaacg cgcacgcacg ccgtccagca
    14521 gcgtttgcat cgccgggccg gaaagcaggc tcggcgtacc accgccaata aagattgtct
    14581 ttacttcacg gccctgagcg taagccacat cgttgtccag atcgcaaagc agatgctgaa
    14641 cgtaatcgtc gtgcggcact tctcctttca acgcgtgaga gttgaaatcg cagtacgggc
    14701 atttctgcac gcaccacggg atgtgaatgt agagactcag cggcggtaat ttaaccatta
    14761 cgtaaagcgt ccaacagcag tttcaatgcc tgaccacggt gggaaatggc gctcttttct
    14821 tcgcgggtca gttcggcagc ggttttccct tcggaaggta cgaagaagat tggatcataa
    14881 ccaaagccac cagtgcccgc tggttcacga gtaatcacgc ccggccagct accgtggcac
    14941 accagcggag tcggatcttc cgcgtgacgc agatatacca gcacgcagtg gaaacgcgcc
    15001 tgacgttggt cgtccggtac gtctttcagt gtttccagta gtttttgcag attctttaga
    15061 tcggtcgcgt cttcaccgga ataacgcgcg gagtaaatgc ccggcgcgcc gccaagcaca
    15121 tctaccgcca gaccagagtc gtcggcaatt gccggtaaac cggtcacttt tgccgcatgg
    15181 cgcgctttca gaatcgcgtt ttcgataaag gtcaggccgg tttcttcagc ggaatctacg
    15241 ccgaggtctg tttgggccac gatatcaaga ccgaagtcgc taagcagcga cgccagctca
    15301 cgcactttac cggcattgcc ggttgcgagg acaacttttt gcataggata cctaattaat
    15361 taacgccgcg atttctggcg ggatttgttg cggattaatg attttaattt gtttgtggcg
    15421 gccaagttcg cctttttcaa tcaccacctg gcttttggca acccggaatt gcttaccgag
    15481 aaacttcacc agatgactgt tggcctggcc gtcaaccggc ggcgcggtaa tggcgacttt
    15541 aacttcgtcg ccatgtaaac cgacaataga atcacggctg gctttcggct gaatatagag
    15601 ccgtaaaacc agaccgtcat cattaactgt tacggcactc ataacgccat ccacagcccc
    15661 ggcagcagca tgtttccggt tgcctgtaat acttctgcga cacccatatt gatgacatac
    15721 agcagcagaa cgaggatcat cggcgagaaa tcaatccccc ccattgccgg tagcaggcgg
    15781 cgaatcgggc gcagcagcgg atcggccagc tgaatcagca cgtattcaat cggactacga
    15841 ccctggctta cccagctcat aatcgccatc accagcagga cccagaaaat cagcaggccg
    15901 atggttttca gcagaatcag taaaccggca atccagatga ttggcaggaa ggtcaccact
    15961 ttaaacagca cgatggcttt gataaaactg agaatgtacg caaccagcag cgaggcgcta
    16021 tcaatgggac ccattgccgg aataacgcgg cgcagtggcc cgataattgg ctgtgttacc
    16081 ttcactacaa actgtgagaa ggggttgtaa aaatcacaat gagcccactg catccagatg
    16141 cgtaataaca gcaccatggt atacagctca atgaccgttg aaagcaggaa agtcaacgta
    16201 ttcatggcgt tcctttaatt ccttattttt tagagtaatc acgcgcacca aaaattgcag
    16261 tgccgatacg aaccatcgtg ctacctgccg caatggcggc ttccatatcg tccgacattc
    16321 ccagagagag cgtgtcgata tgcgggtagc gcgttttcag tccggcaaat gctacagcca
    16381 tttggcgtgc aacttcaaac tgccttacat attctgactc aggcgcaggg attgccatca
    16441 gcccgcgcag acgtaaacgc ggtagttcag cgaccgccgc tgccagctcg tccagttctg
    16501 ccagttgaat cccggactta ctgttttcat cactaatatt aatttgaatc agtacgttaa
    16561 gagggggaag ttctgccggg cgctgatcgt tgaggcgggt agcaatgcgc aaacggtcga
    16621 tggtatgaca ccagtcgaaa tgctctgcca ccaggcggct tttattagac tgcaacgggc
    16681 caataaaatg ccattctaat cctgttacgc ccagttcctg aaagtggcga attttatcta
    16741 ccccttcctg aacgtagttt tcaccaaatt gacgctgccc ggcatcaatg gcttctgcga
    16801 tggcgctcgc aggttttgtt ttactgactg caagcagcgt aatttcttct ggagaacggc
    16861 cgcaacgcgt tgcagccgct gagattttgt cccggacctg tgccaggtta tgcgcaatat
    16921 cgttcatttt ccgaggatct tagtatgaat atggaagaaa ttgtggccct tagtgtaaag
    16981 cataacgtct cggatctaca cctgtgcagc gcctggcccg cacgatggcg cattcgcggg
    17041 ctaatggaag ctgcgccgtt tgatgcgccg gacgtcgaag agctactgcg ggagtggctg
    17101 gatgacgatc agcgggcaat attgctggaa aatggccagc tggattttgc cgtgtcgctg
    17161 gcggaaaacc agcggttgcg tggcagtgcg ttcgcgcaac ggcaaggtat ttctctggca
    17221 ttacggttgt taccttcgca ctgtccacag ctcgaacagc ttggtgcgcc accggtattg
    17281 ccggaattac tcaagagcga gaatggcatg attctggtga cgggggcgac ggggagtggc
    17341 aaatctacca cgctggcggc gatggttggc tatcttaatc aacatgccga tgcgcatatt
    17401 ctgacgctgg aagatcctgt ggaatatctc tatgccagcc agcgatgttt gatccagcag
    17461 cgggaaattg gtttgcactg tatgacgttc gcatcggggt tgcgggccgc attgcgggaa
    17521 gatcctgatg tgattttgct cggagagcta cgtgacagtg agacaatccg tctggcgctg
    17581 acggcggcag aaaccgggca tctggtgctg gcaaccttac atacacgtgg tgccgcgcag
    17641 gcagttgagc gactggtgga ttcatttccg gcgcaggaaa aagaccccgt gcgtaatcaa
    17701 ctggcaggta gtttacgggc agtgctgtca caaaagctgg aagtggataa acaggaagga
    17761 cgcgtggcgc tatttgaatt gctgattaac acacccgcgg tggggaattt gattcgtgaa
    17821 gggaaaaccc accagttacc gcatgttatt caaaccgggc agcaggtggg gatgataacg
    17881 tttcagcaga gttatcagca ccgggtgggg gaagggcgtt tgtgacgttg ccatgcccga
    17941 tgcgcaagct tatcgggcct acaaaggcaa taaatgtctg atgcgacgct aacgcgtctt
    18001 atcagaccta cagccgctgc cagatgtgtg ctggataagg tgttcacgca gcatccggca
    18061 aaatggccgg atgacggtaa cgactatccg ccaggctttt aaatcgcctt aatatccctg
    18121 ctcgaaatag ctttcgagaa taataaccgc agaggcagag tcaactttgc ctttgttgag
    18181 cgcccgatag ccgccctgtt caaacagacc ggaacgggct tccacggtgc taagacgctc
    18241 gtcatggagc tttacttcaa caccgaaacg accatgaata cggttggcga atttacgcgc
    18301 tctggcagtc aatggttgct cggtgccgtc catattcagc ggcaaaccga cgatgatttc
    18361 gtccggctgc cactctttca gtaaacgctc gataatgttc cagtccggcg taccgtcctg
    18421 tgctttaatt gcaggcaaag ggcgagcggt gccggtaatg cgttggccga ccgctacgcc
    18481 aatgcttttg gtgccgaagt cgaaggcgag taaggttcca ctcatcaggc gtgtcctgcc
    18541 acaccaggca tggtgagaat atccacacca atcagttttg ccgcctcgcg ccagcggtcg
    18601 gcaatcggcg ttttgaacag aatattcaga tctgccgggg ccgttagcca cgcgttatcg
    18661 agaatttctt gttccagttg acctttctcc caggaggcat aacccagagc caccaatacg
    18721 tcagacggtt gtttatcggt gccgagcgtt tccagcacat cgcgggaagt ggtcattacc
    18781 gtgttgtctg aaatgcgaat gctggaagca aaattggagg gcggagtatg caaaataaac
    18841 ccgcgatctt cagccagcgg accgccgagc ataaccggtt tatccagacg gattgattca
    18901 tcacgcggct ccggcgtgat cttcagcttt tccagaatcc cttcaatttt gagattttcc
    18961 agcggcttgt tgacgatgat ccccattgca ccattggtat tatgttcgca aatgtagacc
    19021 acggaacgac ggaaaatcgg atcctggaga gcaggcatgg caataagaaa gtgatgctgt
    19081 aaattcattg tcagaggttc tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt
    19141 ggaaagaagg gcgctgtcac tcagagtctc aacgagatcc ttctcgctaa ggtgggttac
    19201 tgctgctgta aacgtgcttc gatggcatcc attaacattc cggtgatcga caccggaaac
    19261 tctgcttcaa tctcacgaat acaggttggg ctggtgacgt taatttcagt cagacggtcg
    19321 ccgatgatat ccagaccaac aaaaatcagc cctttttctt tcagcgtcgg cccgatctga
    19381 cgggcgattt tccagtcact ttccgtcagc ggacgaggtt caccgcgacc accggcagcc
    19441 agattgccac gggtttcgcc cccctgcgga atacgcgcca ggcagtacgg taccggctcg
    19501 ccatccacca ccagcacgcg tttgtcgcca tctttaatgg ctggcaggta attttgcgcc
    19561 atgcagtagc gagtgccatg ctcagtcagg gtttcggcaa tcacgccgag gtttggatcg
    19621 ccttctttca cgcggaaaat cgacgcgccg cccataccgt ccagcggctt aagaataatg
    19681 tcgctgtgtt tctcccagaa cgcttttagc tgcgctttat tgcgcgtaac cagcgtttct
    19741 ggcgttaagt cagagaacca ggcggtaaac agtttctcgt tacagtcgcg caggctctgc
    19801 ggcttgttaa cgatcagcgt ccctttctct tcggcacgtt ccagaatata ggtcgcgtag
    19861 ataaactcgg tatcaaacgg cgggtcttta cgcatcagga tcacatcgag atcggccagc
    19921 ggcagatcct gttcaccgac gaacgaaaac cactcttcgt agttctgctt cacgttcagc
    19981 gtgcgggtat gggcgcgggc ttcgccattg atcagataca gatcgcccat ctccatatag
    20041 tgaagttcgt aaccacgacg ctgtgcttcc agcaacatag cgaaactgga atctttcttg
    20101 atgttgatgt ttgcgatggg gtccatcacg atgccgagct tgatcattat tcttctccgt
    20161 tagcccaaat cgccaaatcg tacttgtagc gcggtaatgg cggtgagcgc agttgtctct
    20221 gtacgcaaaa cgcgaggtcc caacaggata tcagtaaatt gatagcgggc agtcatggca
    20281 atttcatctg ccgataaacc gccttccggg ccaatcagca ggcggacgcg ttcaaccggt
    20341 aacggcaacg tattgatgct gttactggcg cgcgggtgaa gattcagttt cagtccttca
    20401 tcctgctctg cacaccaggc ttccagaacc atcgctgggc ggatttccgg cacccggtta
    20461 cgaccacact gctcacaggc agcaattgca atcttctgcc actgctggag cttcttgttc
    20521 agacgttcac tatccagttt aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt
    20581 acaccgagtt cgatcgattt ctggatcgta aattccattt tttcaccacg cgacatcacc
    20641 tgaccgaggt gaatatgcag cggagattcg cgatcgtcga tcttgccttc cagcaccttc
    20701 acttccacgc tttttttgct ggcgctggta atttcggcgt caaagacctg gttgctaccg
    20761 tcaaacaatt gcaacgcctg ccccggcccc atgcgcagta cgcgcccgat atggttggcg
    20821 gcatcttcgc aaagcgcgat gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg
    20881 gggatacgca tagttaaaaa tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg
    20941 ggttaacaaa aagagtcccg ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg
    21001 cacatacggg ttatgattgc cttgcacctt cgcgatgcgt tcatcgcgct cgcactccca
    21061 gtcggtaacc ggatacatct tgttccatgc gttgaacagc tgcgtttgct ggcgagagag
    21121 tgtcaggttg tattggtcgc gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg
    21181 cgctggtggt tcggcagctt tttctttgaa atcgaccttc atggcgcatt gaccgtactg
    21241 gccttcgccg ccattccact ggctgtacat aaagttgccg cgatcgccat tcacctcgcc
    21301 gactgacggt tgcaggttat gcatatcgct ttccatcttg cgatagaccg gatctttagc
    21361 gcagttttga cgtccaccgt cctgccagca ctgccgctgg tgaccgaatt gccaggcggg
    21421 aacgacgtgt tcccactcta cgcggctggc gcggttttca tttttgcgca cctgatagcc
    21481 gcacgattgc agatcaacaa cgcctttttt gccctgccag ttaattttac atccgcaata
    21541 aaacgtaccg ggcgcgtcag cgtggacttt taccgccgcg gctttcgcct gagaaaaact
    21601 attgatacct tcggccaacg ccgggccgga aaatgctgca ctcagtacca ccgcagcaat
    21661 agacaaataa cggtacatca cttactccgt gttaaaacga gccagcaacg tagcgaaagt
    21721 tgttttggta tgcaatcagc cagatcagga aagttcctga tagttcaggt ggttattttg
    21781 cgaccagctg ttcaccgcag tgaacacagc ggtagacggc ttcgccacga actacgcgat
    21841 tatgacggcg tacggtaagc tgatgctcct ggcacttgca gcggtagggg aaggtgttgc
    21901 ggcgcacgga ttgcagttca aactgatgcg tacgacgggc gggaacgccc agcacgcttt
    21961 ccatcatcca cttccactct ttgccatgtg gcgctacgcg gccgaaatgt ttccatacca
    22021 gcaaatgtgc cagttcgtgc ggcaccactt cttcaataaa ggcttcactg ttttccaaca
    22081 gcaaaacggg attgaggcgg atttcatagc tttccagcca ggctgttccg gcggaggttc
    22141 cgcgctgggt gtaagagagt tttggctccg ggtagttacg ccctagcttc aggttggcct
    22201 gggcgagttt ttcccgcagg cggcgcataa cggcctgttg gatggcgata gggagacggg
    22261 atgttttcat agcggcagag gatagagcga agaaaagatg actgcaagag gcggcttcct
    22321 ccgcgacggg aggaagcctg aggagattaa tcgtgagcgc cgatttcgcg cagtttacga
    22381 cctttcatca gattacgttc aatatgttcc agcgaaacgt gtttggtttc cggtaccagc
    22441 cacaatgtca gcaggataaa cagtacgttc agagccgcat acacccagaa ggtgttggcg
    22501 ttacccagcg tgttgagcat ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag
    22561 ttggtggcgg tggagcaggt gatgccaaaa tcgcggcctt tcagcggctg aatttcggag
    22621 cacagtaccc aaatcagcgg accggcactc atggcaaaac cgacaataaa catcagcagc
    22681 atggcgatgg cgaaatactg cgctgacgga gagtgaatac cgatatgcat cattgtaccg
    22741 agtacgccca tgccagcagc catcaccagg aagcccagcg ttagcgttgg tttacgtccc
    22801 cagcggtcaa caaggccgat tgcgataaag gtggcaagta cgttggtcag gccgacaatc
    22861 actgtccccc acatttgctc tgtggtgttg gtataacccg ccagttcgaa gattttcggc
    22921 gcgtaataca tgatgacgtt catcccggtg aattgctgca ttacctgcaa cagtacgcca
    22981 aggaacaccg cgcggcggaa gttgctgttc tctttaaaca gcgcccagcc actctgttta
    23041 acctgcaaac tttcacggat ttcatccagt tcgcgtttcg cttccgcgct ggtgtcgcgc
    23101 aggcgtagca gcacgcgttc ggcatcaaca aaacggcgtt tggcggcaaa ccaacgtggg
    23161 ctgtctggca ggaagaagac accaatcagc agcaaaattg ccgggatgat aatcacaccc
    23221 agcatccagc gccatgcacc ggtgtagctg aaggcggtat cagaaagata agcaccgagg
    23281 atcccgatag tgatcatcaa ctgatacatc gagatcatac tgccacgaat tttttccggc
    23341 gcaatttcag agaggtacag cggtgcggta taagaggcca cacccaccgc cagccccagt
    23401 agaacgcggg aaagaatcag tacttcaacg tttggcgcag ccgcagagaa cagcgaaccg
    23461 gcaacaaaca aaattgcgcc gatcatcagg ctctttttgc gcccgagttt aaaggagagc
    23521 cagccgctgc ccaccgcacc gactgccgca ccgaacatca tggaacttac gacccattct
    23581 tgcgtgtgcg aagtaatctg gaattcatct gcaataaacg gcagtgcgcc agcaattaca
    23641 ccgatatcca ggccaaagag taatcccgcc agagcggcaa ggaagcagac gaaaaacgtc
    23701 attgccttgt ttgaccgccc ctgtttttta gcgtcaggca tgatgccctc caatatggtt
    23761 attttttatt gtgaatataa gataggtgag tacgacgtaa aaagatgtga agcaaatcac
    23821 atcagtgtaa tcgcttacac taacatttat cttagttatg gtttaagtgc atgaaaataa
    23881 ttgagttata tcatcataga ttaaacgctg ttatctgcaa ttaagacttt actgaaaaga
    23941 aatgtaacaa ctgtgaaaac tgaataataa tatgaaaata ttacaaatat ttggctggtg
    24001 ttattggtgt aatcggtttc agtagtgatg ataatttgcg ataaaacgtg ttgaggaaga
    24061 atgatgttat tgtgcgggca gctttttgcc ggatgcggcg tgaatgccta tccaccgcgg
    24121 tgaggtaaaa gaaaaaggcc agcgcgaggc tgtaatgcca gtcagttaag caactgactg
    24181 gctctttttc ggggctgtgg ggtatttcca gggcctctcc tttaccactc tcgggaaggc
    24241 cctttccctt cttgtcggta atttcacaag ttgtcccata cttgcaagat cgtgcatcag
    24301 ctccggtata cgtcccggtg aagcgccctg caatgtcatc agcattctca tcaccattcc
    24361 gcatgattct gagaaactca gttgattcgg ccagtaacct ttcagatgtt ccgccatttt
    24421 aatcatctga tatctcacca gattataagc cagtaagaca ccccacagct cttgctccac
    24481 aagctccggc tttttacttc tcagcgtcag cctgctcagt tgcatcgtct gttttatctc
    24541 cctgtatccc agttcgattt cccagcgatg actgtacaga tccgccattt ctcctccggg
    24601 gaagcgcatg gcgtccgtca tcgacgtcag cagatggcag acttttcctt tgcgcgtcac
    24661 ggtcagcagg cgggctgtca cctcatttcc cagtcccggc cacttttttc gtgcctgcgg
    24721 gctggttttc agcttcacca gatgatcgcc tttacccagt tttctgatct cttcatattg
    24781 cgctcccttt ctgagaggga tcatccagtg gcggtgttct cccgccaggc tcaaggcatt
    24841 taacagtccc agtgagtaat aacctttgtc cattaacgtc agagtgttat cgccggtttg
    24901 ttctataagt tgctcagcaa gctcattttc gctgttcttc atcgtgccga aggctgcagc
    24961 cgtcagcaga tggctggtca gttccatctg gcagaccatt ttgacctgcg ggtagagcgc
    25021 cgggttcccg gcatgtgtct ggcgggggaa ggctgcatcg ttctctggtg tatccggtgt
    25081 gcgccagaac acaccatcga tggccagcag ggtcaggccg caccattgcg gatgcggcgt
    25141 ggcgttatgc cagagctgcg ctgttttcgt gaacacgcgg cggacagcct cacttcccag
    25201 gcgctggcgg gcctgaataa cggcactggg ggcaacgaag gggcgattgc ccggcagcat
    25261 gatgtccagg cggttcacaa tctggtgaag aggttcttta cgctcaagcg ccatgccaac
    25321 aatacaccag accatcattt cgaggggaag acggcgcttg cgtagcgtta cagtacctga
    25381 ttcggcaagg caacgagaga tgagttcggg gtcgaggtaa tcccccagag aagtcagtgg
    25441 gttacgcaga gaatcgtaac gggataccaa atcaagggcc tgtccaatgt gcataaaaaa
    25501 atccggaaac gagtgagcgt ttccggattc ttacacagcc actggatcgg tcaactgatc
    25561 cttaactgat cggcattaca gcgcgaggct ggcctttgaa agcaggtgaa gaaagattac
    25621 ttcagaccgg cagcatcgcg cagcagctgc gctttgtcgg ttttttccca cgggaaatgt
    25681 tcacgaccaa agtgaccgta tgctgcggtt tctttgtaga tcgggtgcag cagatccagc
    25741 atctgaatca gaccgtatgg gcgcaggtcg aagaactcac gtaccagcag ggtcagctgt
    25801 tcagaaggca ctttctcagt accgaaagtt tctaccatga tggaggtcgg ttcagccacg
    25861 ccgattgcgt aggaaacctg aatttcacaa cgatcggcca ggccagcagc aacgatgttt
    25921 ttcgcgacat aacgtgctgc gtaggctgcg gaacggtcca cttttgatgg atctttacca
    25981 gagaatgcac cgccaccgtg acgtgccatg ccgccgtagg tatcaacgat aattttacga
    26041 ccagtcagac cgcagtcacc catcgggcca ccgataacga aacgaccagt cgggttgatg
    26101 aagaatttgg tggcagaagt cagccattca gcgggcagaa ttggcttgat gatctcttcc
    26161 attaccgctt cttgcagcga tttctggtcg atctcttcag agtgctgagt ggaaagcacg
    26221 acagcatcaa taccaacgat tttgccgtcg tcatactgga aagtcacctg gcttttcgcg
    26281 tccgggcgca gccacggcag agtaccgttt ttacgcactt cagcctgacg ctgtaccaga
    26341 cggtgtgcat aggtgatagg tgctggcatc agcacgtcgg tttcattagt tgcgtagcca
    26401 aacatcagac cctggtcacc cgcgccctgt tccagcggat cggcacggtc aacgccctgg
    26461 ttgatgtcag gagactgttt gccgatagca ctcagaaccg cacaggagtt agcgtcaaag
    26521 cccatgtcgg aatgtacata gccaatttcg cgaacggtgt tacgggtgat ctcttcgatg
    26581 tctacccagg cgctagtggt gatttcgccg ccaactaaaa ccatgccggt ttttacgtag
    26641 gtttcgcaag caacgcgtgc tttcggatcc tgttcgagga tcgcgtctaa aacggcatca
    26701 gagatttggt cagcaatttt gtcaggatgc ccttcagaga cggactcgga cgtaaaaagg
    26761 tgttttgcca tatttaatat cacctaaaga gaatttggtt agctcaaact gttgtgtgga
    26821 ttttctgtgg tagcggatcc taccacgact ctgcaggtta aaaacactgg cagtctgagt
    26881 gttaatcggt atggatggat taacatctgg atggctattt taggtcaatt cttcacccga
    26941 tttccacttt tttttgaatc gtgtctcatt ctgttaaaaa cgtggctgga aatttttcct
    27001 gacaatgccg gcattctgcg tatttatctt ttgcaatttt ctgccattgt ggggtataaa
    27061 acgcggcgcg cggcttaaat aaaaagcaca cgacgtttct ttcgtgttgc cacttccagc
    27121 cgggttgaaa tcagagtttt ggcttgtggg ttcgtcttaa caggcggccg tggaggtgat
    27181 acgaaataat gaaccgttgt ctgctgctta acctgtctca ccgttctggt gaagattcgt
    27241 tccccgcact ctgcatctct gctttgcata cctgccgatg ttatacccat ctcggcgctt
    27301 ctcaggattc aagagctggt tacagttact gaggactgaa caagggcgct cttgtaaaaa
    27361 caagagtttt ctcgtggttt cgccgaactg tcacacttac gttcggttat gtgcttaata
    27421 atgttatgaa aaagaaaccg gttgcgcagt tggagcgtca gcattcactg ctggaaaatc
    27481 catgtgctta tgggttgtta tcgcagttcc aggctgcgat agtcgttaac tgttttacac
    27541 ttaataaaat aatttgaggt tcgctatgtc tgacgacatg tctatgggtt tgccttcgtc
    27601 agcgggcgaa cacggtgtac tacgctccat gcaggaggtt gcaatgagct cccaggaagc
    27661 cagcaagatg ctgcgtactt acaatattgc ctggtggggc aataactact atgacgttaa
    27721 cgagctgggc cacatcagcg tgtgcccgga cccggacgtc ccggaagctc gcgtcgatct
    27781 cgcgcagtta gtgaaaactc gtgaagcaca gggtcagcgt ctgcctgcac tgttctgttt
    27841 cccacagatc ctgcagcacc gtttgcgttc cattaacgcc gcgttcaaac gtgcgaggga
    27901 atcctacggc tataacggcg attacttcct tgtttatccg atcaaagtta accagcaccg
    27961 ccgcgtgatc gagtccctga ttcattcggg cgaaccgctg ggtctggaag ccggttccaa
    28021 agccgagttg atggcagtgc tggcacatgc tggcatgact cgtagcgtca tcgtctgcaa
    28081 cggttataaa gaccgcgaat atatccgcct ggcattaatt ggcgagaaga tggggcacaa
    28141 ggtctatctg gtcattgaga agatgtcaga aatcgccatt gtgctggatg aagcagaacg
    28201 tctgaatgtc gttcctcgtc tgggcgtgcg tgcacgtctg gcttcgcagg gctcgggtaa
    28261 atggcagtcc tccggcgggg aaaaatcgaa gttcggcctg gctgcgactc aggtactgca
    28321 actggttgaa acgctgcgtg aagctgggcg tctcgacagc ctgcaactac tgcacttcca
    28381 cctcggttcg cagatggcga atattcgcga tatcgcgaca ggcgttcgtg aatccgcgcg
    28441 tttctatgtg gaactgcaca agctgggcgt caatattcag tgcttcgacg tcggcggcgg
    28501 tctgggcgtg gactatgaag gcacccgttc gcagtctgac tgctccgtaa actacggcct
    28561 caatgaatat gccaacaaca tcatctgggc gattggtgat gcgtgtgaag aaaacggtct
    28621 gccgcatccg acggtaatca ccgaatcggg tcgtgcggtg actgcgcatc acaccgtgct
    28681 ggtgtctaat atcatcggcg tggaacgtaa cgaatacacg gtgccgaccg cgcctgcaga
    28741 agatgcgccg cgcgcgctgc aaagcatgtg ggaaacctgg caggagatgc acgaaccggg
    28801 aactcgccgt tctctgcgtg aatggttaca cgacagtcag atggatctgc acgacattca
    28861 tatcggctac tcttccggca tctttagcct gcaagaacgt gcatgggctg agcagcttta
    28921 tttgagcatg tgccatgaag tgcaaaagca gctggatccg caaaaccgtg ctcatcgtcc
    28981 gattatcgac gagctgcagg aacgtatggc ggacaaaatg tacgtcaact tctcgctgtt
    29041 ccagtcgatg ccggacgcat gggggatcga ccagttgttc ccggttctgc cgctggaagg
    29101 gttggatcaa gtgccggaac gtcgcgcagt gctgctggat attacctgtg actctgacgg
    29161 tgctatcgac cactatattg atggtgatgg tattgccacg acaatgccaa tgccggagta
    29221 cgatccagag aatccgccga tgctcggttt ctttatggtc ggcgcatatc aggagatcct
    29281 tggcaatatg cacaacctgt tcggtgatac cgaagcggtt gacgtgttcg tgttccctga
    29341 cggtagcgta gaagtagaac tgtctgacga aggcgatacc gtggcggaca tgctgcaata
    29401 tgtacagctc gatccgaaaa cgctgttaac ccagttccgc gatcaagtga agaaaaccga
    29461 tcttgatgct gaactgcaac aacagttcct tgaagagttc gaggcaggtt tgtacggtta
    29521 tacttatctg gaagatgagt aagtccgtat tacttgaatc cgcttaattt agcggtgata
    29581 atccgccaca atttattgtg acaaatccaa cccttcctcg tcgggcctaa cgacgcggaa
    29641 gggttttttt atatcgactt tgtaatagga gtccatccat gagcacctta ggtcatcaat
    29701 acgataactc actggtttcc aacgcctttg gttttttacg cctgccgatg aacttccagc
    29761 cgtatgacag cgatgctgac tgggtgatta ctggtgtgcc gttcgatatg gccacttctg
    29821 gtcgtgcggg gggacgccac ggtccggcag cgatccgtca ggtttcgacg aatcttgcct
    29881 gggaacacaa tcgcttcccg tggaatttcg acatgcgtga gcgcctgaac gtcgtggact
    29941 gcggcgatct ggtatatgcc tttggcgatg cccgtgagat gagcgaaaaa ctgcaggcac
    30001 acgccgagaa gctgctggct gccggtaagc gtatgctctc tttcggtggt gaccactttg
    30061 ttacgctgcc gctgctgcgt gctcatgcga agcatttcgg caaaatggcg ctggtacact
    30121 ttgacgccca caccgatacc tacgcgaacg gttgtgaatt tgaccacggc accatgttct
    30181 ataccgcgcc gaaagaaggt ctgatcgacc cgaatcattc cgtgcagatt ggtattcgta
    30241 ccgagtttga taaagacaac ggttttaccg tgctggacgc ctgccaggtg aacgatcgca
    30301 gcgtggatga cgttatcgcc caagtgaaac agattgtggg tgatatgccg gtttacctga
    30361 cctttgatat cgactgcctg gatcctgctt tcgcgccggg aaccggtacg ccagtgattg
    30421 gcggcctgac ctccgatcgc gccattaaac tggtacgcgg cctgaaagat ctcaacatcg
    30481 ttgggatgga cgtagtggaa gtggctccgg catacgatca gtcggaaatc accgctctgg
    30541 ctgcggcgac gctggcgctg gagatgctct atattcaggc ggcgaaaaag ggcgagtaag
    30601 caccagatgc gatgcgcacg ggtaaaacgt gccattaatg tcggatgcgg cgtgaacgcc
    30661 ttatccgacc tacgttcggc acccgtaggc cggataagat gcgccagcat cgcatccggc
    30721 aatgcgcaca gggtaacaaa tgtgccattc atgtcagaag gggcgtgaac gccttatctg
    30781 acctacgttc gacaccacca ggctttactt aatcccatct gcgctcatac gatcgcgaat
    30841 atgctgggcg cgttcggcgg atgcaggatg gtcgtcaaac attgagcttt ggcgaccttc
    30901 ttccagtttt gccagttttt caaagctggt ggcaagacct gccgggctga tgccgcgttg
    30961 gcgcagaaga tcgtaagaat aatcatcggc ttctgcttcc tggcgctggg agaattgcga
    31021 attgactaat ttctcgccca gatcaccgag ttgtgattga gataaactgc cgacaatccc
    31081 gcccgcagag gccgcagcta ctcgcacggc atttgtacca agtgccacct gcattccttt
    31141 tttcacatgg cctaatgcca cgtgccccat ttcgtgaccg atcaccgctt cgacttcgtt
    31201 atccgtcatc atatccatca gcccgctata gacacggata cagccgttag ccattgcaaa
    31261 ggcgttcaca tccttcgcca tatacacttt gtaatttacc ggctgaccgt tgatattgtt
    31321 gcctagcgca ttggcaatag ttgtcagacg tttagcgtat tcgctattgg ctggcgcaat
    31381 cgtcgccttg ctgtccatct cctgacatgc ctgatcgctc agggttttca cctgcgcatc
    31441 actcaaactg taagcctgaa aagcttccgc tcctgatgag agcagtccgt tggagtccat
    31501 attctggcaa ccagtcagta ccgttgccac gctcattgct accaataagg cgcgaatttt
    31561 catgttttct cttccgcaca aggtcaggat tattctaaaa atgcgccgtt tgcaggtgaa
    31621 tcgacgctca gtctcagtat aaggaaaagc gcagcggacg ggcgagtaga ttgcgcaaca
    31681 tgcgagcatg atccagagat ttctgaagcg gcaaaaggat gttccatgta catgacgcgc
    31741 ggcttgcggt aaattgttgg caaattttcc ggcgtagccc aaaacgcgct gtcgtcaagt
    31801 cgttaagggc gtgcccttca tcatccgatc tggagtcaaa atgtcctcac gtaaagagct
    31861 tgccaatgct attcgtgcgc tgagcatgga cgcagtacag aaagccaaat ccggtcaccc
    31921 gggtgcccct atgggtatgg ctgacattgc cgaagtcctg tggcgtgatt tcctgaaaca
    31981 caacccgctg aatccgtcct gggctgaccg tgaccgcttc gtgctgtcca acggccacgg
    32041 ctccatgctg atctacagcc tgctgcacct caccggttac gatctgccga tggaagaact
    32101 gaaaaacttc cgtcagctgc actctaaaac tccgggccac ccggaagtag gttataccgc
    32161 tggtgtggaa accaccaccg gtccgctggg tcagggtatt gccaacgcag tcggtatggc
    32221 gattgcagaa aaaacgctgg cggcgcagtt taaccgtccg ggccacgaca ttgtcgacca
    32281 ctacacctac gccttcatgg gcgacggctg catgatggaa ggcatctccc acgaagtttg
    32341 ctctctggcg ggtacgctga agctgggtaa actgattgca ttctacgatg acaacggtat
    32401 ttctatcgat ggtcacgttg aaggctggtt caccgacgac accgcaatgc gtttcgaagc
    32461 ttacggctgg cacgttattc gcgacatcga cggtcatgac gcggcatcca tcaaacgcgc
    32521 agtagaagaa gcgcgcgcag tgactgacaa accgtccctg cttatgtgca aaaccatcat
    32581 cggtttcggt tccccgaaca aagccggtac ccacgactcc cacggtgcgc cgctgggcga
    32641 cgctgaaatt gccctgaccc gcgaacagct gggctggaaa tacgcgccgt tcgaaatccc
    32701 gtctgaaatc tatgctcagt gggatgcgaa agaagcaggc caggcgaaag aatccgcatg
    32761 gaacgagaaa ttcgctgctt acgcgaaagc ttatccgcag gaagccgctg aatttacccg
    32821 ccgtatgaaa ggcgaaatgc cgtctgactt cgacgcgaaa gcaaaagagt tcattgctaa
    32881 actgcaggct aacccggcga aaatcgccag ccgtaaagcg tctcagaatg ctatcgaagc
    32941 tttcggtccg ctgttgccgg aattcctcgg cggttctgct gacctggcac cgtctaacct
    33001 gaccctgtgg tctggttcta aagcaatcaa cgaagatgct gcgggtaact acatccacta
    33061 cggtgttcgc gagttcggta tgaccgcgat tgctaacggt atctccctgc acggtggttt
    33121 cctgccgtac acctccacct tcctgatgtt cgtggaatac gcacgtaacg ccgtacgtat
    33181 ggctgcgctg atgaaacagc gtcaggtgat ggtttacacc cacgactcca tcggtctggg
    33241 cgaagacggc ccgactcacc agccggttga gcaggtcgct tctctgcgcg taaccccgaa
    33301 catgtctaca tggcgtccgt gtgaccaggt tgaatccgcg gtcgcgtgga aatacggtgt
    33361 tgagcgtcag gacggcccga ccgcactgat cctctcccgt cagaacctgg cgcagcagga
    33421 acgaactgaa gagcaactgg caaacatcgc gcgcggtggt tatgtgctga aagactgcgc
    33481 cggtcagccg gaactgattt tcatcgctac cggttcagaa gttgaactgg ctgttgctgc
    33541 ctacgaaaaa ctgactgccg aaggcgtgaa agcgcgcgtg gtgtccatgc cgtctaccga
    33601 cgcatttgac aagcaggatg ctgcttaccg tgaatccgta ctgccgaaag cggttactgc
    33661 acgcgttgct gtagaagcgg gtattgctga ctactggtac aagtatgttg gcctgaacgg
    33721 tgctatcgtc ggtatgacca ccttcggtga atctgctccg gcagagctgc tgtttgaaga
    33781 gttcggcttc actgttgata acgttgttgc gaaagcaaaa gaactgctgt aattagcatt
    33841 tcgggtaaaa aggtcgcttc ggcgaccttt tttattacct tgatatgtcc gtttgcggac
    33901 aagcaataga taaggcgtgt tgtagatcac aaatatttat atgca
//
LOCUS       DAOFQD010000046        33452 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.046, whole genome shotgun sequence.
ACCESSION   DAOFQD010000046 DAOFQD010000000
VERSION     DAOFQD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.046"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..801)
                     /gene="waaO"
                     /locus_tag="QBF46_002189"
                     /gene_synonym="rfaI"
     CDS             complement(<1..801)
                     /gene="waaO"
                     /locus_tag="QBF46_002189"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HDQ6674368.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLANSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYV"
     gene            complement(817..1614)
                     /gene="rfaP"
                     /locus_tag="QBF46_002190"
     CDS             complement(817..1614)
                     /gene="rfaP"
                     /locus_tag="QBF46_002190"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDQ6674369.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(1607..2731)
                     /locus_tag="QBF46_002191"
     CDS             complement(1607..2731)
                     /locus_tag="QBF46_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDQ6674370.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(2728..3786)
                     /gene="rfaQ"
                     /locus_tag="QBF46_002192"
     CDS             complement(2728..3786)
                     /gene="rfaQ"
                     /locus_tag="QBF46_002192"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDQ6674371.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            4199..5476
                     /gene="waaA"
                     /locus_tag="QBF46_002193"
     CDS             4199..5476
                     /gene="waaA"
                     /locus_tag="QBF46_002193"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDQ6674372.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            5484..5963
                     /gene="coaD"
                     /locus_tag="QBF46_002194"
     CDS             5484..5963
                     /gene="coaD"
                     /locus_tag="QBF46_002194"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDQ6674373.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(6002..6811)
                     /gene="mutM"
                     /locus_tag="QBF46_002195"
     CDS             complement(6002..6811)
                     /gene="mutM"
                     /locus_tag="QBF46_002195"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDQ6674374.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(6909..7076)
                     /gene="rpmG"
                     /locus_tag="QBF46_002196"
     CDS             complement(6909..7076)
                     /gene="rpmG"
                     /locus_tag="QBF46_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDQ6674375.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(7097..7333)
                     /gene="rpmB"
                     /locus_tag="QBF46_002197"
     CDS             complement(7097..7333)
                     /gene="rpmB"
                     /locus_tag="QBF46_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDQ6674376.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(7550..8218)
                     /gene="radC"
                     /locus_tag="QBF46_002198"
     CDS             complement(7550..8218)
                     /gene="radC"
                     /locus_tag="QBF46_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDQ6674377.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMRAESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            8390..9610
                     /gene="coaBC"
                     /locus_tag="QBF46_002199"
     CDS             8390..9610
                     /gene="coaBC"
                     /locus_tag="QBF46_002199"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDQ6674378.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKIPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            9588..10046
                     /gene="dut"
                     /locus_tag="QBF46_002200"
     CDS             9588..10046
                     /gene="dut"
                     /locus_tag="QBF46_002200"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDQ6674379.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            10153..10749
                     /gene="slmA"
                     /locus_tag="QBF46_002201"
     CDS             10153..10749
                     /gene="slmA"
                     /locus_tag="QBF46_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDQ6674380.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10786..11427)
                     /gene="pyrE"
                     /locus_tag="QBF46_002202"
     CDS             complement(10786..11427)
                     /gene="pyrE"
                     /locus_tag="QBF46_002202"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDQ6674381.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(11492..12208)
                     /gene="rph"
                     /locus_tag="QBF46_002203"
     CDS             complement(11492..12208)
                     /gene="rph"
                     /locus_tag="QBF46_002203"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDQ6674382.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            12335..13198
                     /locus_tag="QBF46_002204"
     CDS             12335..13198
                     /locus_tag="QBF46_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDQ6674383.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            13419..14243
                     /gene="dinD"
                     /locus_tag="QBF46_002205"
     CDS             13419..14243
                     /gene="dinD"
                     /locus_tag="QBF46_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDQ6674384.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            14534..15151
                     /locus_tag="QBF46_002206"
     CDS             14534..15151
                     /locus_tag="QBF46_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDQ6674385.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(15148..16830)
                     /gene="ligB"
                     /locus_tag="QBF46_002207"
     CDS             complement(15148..16830)
                     /gene="ligB"
                     /locus_tag="QBF46_002207"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDQ6674386.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGAKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            17088..17711
                     /gene="gmk"
                     /locus_tag="QBF46_002208"
     CDS             17088..17711
                     /gene="gmk"
                     /locus_tag="QBF46_002208"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDQ6674387.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17766..18041
                     /gene="rpoZ"
                     /locus_tag="QBF46_002209"
     CDS             17766..18041
                     /gene="rpoZ"
                     /locus_tag="QBF46_002209"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDQ6674388.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            18060..20168
                     /gene="spoT"
                     /locus_tag="QBF46_002210"
     CDS             18060..20168
                     /gene="spoT"
                     /locus_tag="QBF46_002210"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDQ6674389.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPSPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            20175..20864
                     /gene="trmH"
                     /locus_tag="QBF46_002211"
     CDS             20175..20864
                     /gene="trmH"
                     /locus_tag="QBF46_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDQ6674390.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            20870..22951
                     /gene="recG"
                     /locus_tag="QBF46_002212"
     CDS             20870..22951
                     /gene="recG"
                     /locus_tag="QBF46_002212"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDQ6674391.1"
                     /translation="MKGRLLDTVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(23117..24322)
                     /gene="gltS"
                     /locus_tag="QBF46_002213"
     CDS             complement(23117..24322)
                     /gene="gltS"
                     /locus_tag="QBF46_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDQ6674392.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLCAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            24602..25993
                     /gene="xanP"
                     /locus_tag="QBF46_002214"
     CDS             24602..25993
                     /gene="xanP"
                     /locus_tag="QBF46_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDQ6674393.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            26114..27823
                     /locus_tag="QBF46_002215"
     CDS             26114..27823
                     /locus_tag="QBF46_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDQ6674394.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(27876..30194)
                     /gene="yicI"
                     /locus_tag="QBF46_002216"
     CDS             complement(27876..30194)
                     /gene="yicI"
                     /locus_tag="QBF46_002216"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDQ6674395.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(30204..31586)
                     /locus_tag="QBF46_002217"
     CDS             complement(30204..31586)
                     /locus_tag="QBF46_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDQ6674396.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            31879..31973
                     /locus_tag="QBF46_002218"
     tRNA            31879..31973
                     /locus_tag="QBF46_002218"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31913..31915,aa:Sec,seq:tca)
     gene            32136..32978
                     /locus_tag="QBF46_002219"
                     /pseudo
     CDS             32136..32978
                     /locus_tag="QBF46_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218976.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            32977..33180
                     /locus_tag="QBF46_002220"
                     /pseudo
     CDS             32977..33180
                     /locus_tag="QBF46_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT         8403 a         8870 c         8265 g         7914 t
ORIGIN      
        1 gacataatga attaatacgg tttcatcatt aattggacaa acaaatgatt tttttaattc
       61 ataatttaaa ctaaattgcg tattgtattt agcatcaatg aatttaactt tccctaacag
      121 gataagatta agaatatctt gatccatata ggttaaacgg gaaacgatgg ctttatcagc
      181 aagcatcgac atcgctttag cagaaacgga ctcctgcgcc cacgctagtg tattaattaa
      241 caggacacct gaattaaagt aacccttttc aagttcatta cactgcagac tttgaccccg
      301 taacgaccac cagttagcat cgcgttcagt aacaacagca gcaacgttat tggcaagatc
      361 cattgttatc agcggcttta agtttccctg acaggcgata tcagcatcca ggtaaaggat
      421 cttatcttgt tgatcaataa agtaatctgc aattacaaaa cggaaataca tggcaataga
      481 ccaatttttg gtcgtcggta atgccttaag ccgttcacaa tttactagat ggatctggat
      541 acaggtacga tagctattcg ccaattgggc taaacgctgg atatcggctt cagggatatc
      601 atcaataaaa acgtggaaaa caaaactcac gtcgttgtta tgtaacaaaa ctgacgtgat
      661 tgaaacacca caaccaaaaa gaaagttttt atcaatgcca taagcaacat ggaatgatga
      721 tgccactgac gccgctggcc tttcatcgaa gatgattgtc ttattgatca tctcttgtgg
      781 attaaaatag tgggcactca tatttctctc cggaaattac aacgattttc gaatcgttct
      841 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
      901 aatgtcttta agcggggcgg caaaatacac tttcataaac cgccagatat cccgctgagt
      961 caggccgata ttcatcgatg aaaaataaag cccaataaga tctttatcgc gccaacgacg
     1021 tggaacgcgc gtgcgaagct gcgcccggtg caggtcaatt accgaaattt ttaactcctc
     1081 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacggtggtt
     1141 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
     1201 tacgcgaaca tctggagggt tagtcgccca gtcagcacag taatcttcca gacttatggt
     1261 tggtgtcaga tcttcggtaa taataaatga agttctggtc agcggattca tgcctttttc
     1321 gccaaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
     1381 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
     1441 tttcagggtc gtgccgcgat gccatttgag aaaatagctt ttgcccgcca tttcaaagcg
     1501 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaagttt taacttcctc
     1561 aaaaggatct tttccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
     1621 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
     1681 aatgtcgcgc attttccgcc caggcctggc gcaatgaaga ttgcgttaac gctttgcgta
     1741 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattggcct
     1801 cgacaatata atgcgcatag ccacaaacgg ctgttgttag taccggtaat cctgcagtaa
     1861 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
     1921 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
     1981 cgccgcgttt ttctgccagc gcctcaaatt ttcgcggttt atcctgccca acaacatata
     2041 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcga
     2101 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
     2161 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
     2221 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
     2281 aatcggcgat ttgcttatct gtcagcatca gtagctgtgt cggtttgccc tgttcgaagg
     2341 tagcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
     2401 tctcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
     2461 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
     2521 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
     2581 gttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
     2641 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
     2701 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aagagggcag
     2761 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
     2821 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
     2881 gttatttgac cagggacgcc agaaaatatg gtctgtcgca ccaaacagcg atatcagcgg
     2941 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
     3001 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
     3061 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
     3121 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
     3181 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
     3241 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
     3301 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
     3361 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
     3421 agactgccga tgatgataat cctgggaaat tttcacacgg gcatttaata agcgaaccag
     3481 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
     3541 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
     3601 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
     3661 aagcagcaca tcgatttttg cgtcagggta attttttttc agcgaactaa tgacgggagt
     3721 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
     3781 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
     3841 taccgcccca ggctcaacag ctacctgaat actgataccg cactgatatt gacgcgctag
     3901 tgtatcactt ctttcgcggc aatccgaccc gaatgatttt taagcacatt gttgattttc
     3961 aataaagaaa tcaatcaaat aaaatcatta gcaatcatat tctttcactg ttgctctgcg
     4021 attccttcct gcacgtacag cagaaatcaa tgaatcgcat aaaaatacaa aaaaagtata
     4081 gatgttcagc aagaattggc gggtaaattg ctatttaagt caaaaacggg aaaagtaatg
     4141 gtaaagccac agctaaatac atagaatccc cagcacatcc ataagtcagc tatttactat
     4201 gctcgaattg ctttacaccg cccttctcta ccttattcag ccgctgatct ggatacggct
     4261 ctgggtgcgc ggacgtaagg ctccggccta tcgaaaacgc tggggtgaac gttacggttt
     4321 ttaccgccat ccgctaaaac caggcggcat tatgctgcac tccgtctccg tcggtgaaac
     4381 tctggcggcg atcccgttgg tgcgcgcgct gcgccatcgt tatcctgatt taccgattac
     4441 cgttacaacc atgacgccaa ccggttcgga gcgcgtacaa tcggctttcg ggaaggatgt
     4501 tcagcacgtt tatctgccgt atgacctgcc cgatgcgctc aaccgtttcc tgaataaagt
     4561 cgaccccaaa ctggtgttga ttatggaaac cgaactatgg cctaacctga ttgcggcgct
     4621 acacaaacgt aaaattccgc tggtgatcgc taacgcgcga ctctctgccc gctcagccgc
     4681 aggttatgcc aaactgggta aattcgtccg tcgcttgctg cgtcgtatta cgctgattgc
     4741 tgcgcaaaat gaagaagatg gtgcacgttt tgtggcgctg ggcgcaaaaa ataatcaggt
     4801 gaccgttacc ggtagcctga aattcgatat ttctgtaacg ccgcagttgg ctgctaaagc
     4861 cgtgacgctg cgccgccagt gggcaccaca ccgcccggta tggattgcca ccagcactca
     4921 cgaaggcgaa gagagtgtgg tgatcgccgc acatcaggca ttgttacagc aattcccgaa
     4981 tttattgctc atcctggtac cccgtcatcc agaacgcttc ccggatgcga ttaatcttgt
     5041 ccgccaggct ggactaagct atatcacacg ctcttcaggg gaagtcccct ccaccagcac
     5101 gcaggttgtg gttggcgata cgatgggcga gttgatgtta ctgtacggca ttgccgatct
     5161 cgcctttgtt ggcggttcac tggttgaacg tggtgggcat aatccgctgg aagctgccgc
     5221 acacgctatt ccggtattga tggggccgca tacttttaac tttaaagaca tttgcgcgcg
     5281 gctggagcag gcaagcgggc tgattaccgt taccgatgcc actacgcttg caaaagaggt
     5341 gtcctcttta ctcaccgacg ccgattaccg tagtttctat ggccgtcatg ccgttgaagt
     5401 actgtatcaa aaccagggcg cgctacagcg tctgcttcaa ctgctggaac cttacctgcc
     5461 accgaaaacg cattgaggtt gttatgcaaa aacgggcgat ttatccgggt actttcgatc
     5521 ccattaccaa tggtcatatc gatatcgtga cgcgcgccac gcaaatgttc gatcacgtta
     5581 ttctggcgat tgccgccagc cccagtaaaa aaccgatgtt taccctggaa gagcgtgtgg
     5641 cgctggcaca gcaggcaacc gcgcatctgg ggaacgtgga agtggtcggg tttagtgatt
     5701 tgatggcgaa cttcgcccgt aatcaacacg ctacggtgct gattcgtggc ctgcgtgcgg
     5761 tggcggattt tgaatatgaa atgcagctgg cgcatatgaa tcgccactta atgccggaac
     5821 tggaaagtgt gtttctgatg ccgtcgaaag agtggtcgtt tatctcttca tcgttggtga
     5881 aagaggtggc gcgccatcag ggcgatgtca cccatttcct gccggagaat gtccatcagg
     5941 cgctgatggc gaagttagcg tagcgtttat gccggatggt atgccatccg gcgcgcatga
     6001 attacttctg gcactgccga caataaaacg ttgcccgctg cgcatgttta gtcgccacaa
     6061 tcggcgtacc gcacacccga cacggctcgc cttttcgccc gtaaacctgc aattcctgtg
     6121 cgaaatagcc cggtttacca tcactttgca gaaaatcttt cagcgttgta ccgccctgct
     6181 caatcgaacg cagcaacacc gctttaatca cccgagctaa caattcacac tctgccagcg
     6241 acagtgatga cgccagccga tccggatgga tccccgccgc aaacagtgat tcgctggcat
     6301 agatattccc tacccctacc accagcttgt tatccatcag ccacggttta atcgccgttt
     6361 ttttcttcgc gcacttctga tgcagatact caccattgaa atcgtcgctg agcggctccg
     6421 gtccaagatg ggccagcaca ttatgccctt ccagctcttt ggtccacagc caggcaccaa
     6481 agcggcgcgg atcggtgtag cgcagcactt taccgttgct catcaccaaa tccacatggt
     6541 catgcttttc agggggaagt tcttctggaa ggatgcgcag gctgccagac atccccaaat
     6601 gaatgataat ccagccctca ggcagctcca gcagcaaata tttagcccgc cgctgcacgc
     6661 taagcactgg ttggtcgctt aaacggtaga tctcttctga aaccggccag cgcaagcgtc
     6721 cgttgcgcac cactgcatga agaatggttg caccaacgag atgcggttct atgccgcgac
     6781 ggctggtttc aacttcgggt aattcaggca tagcatctcc aggaatgaac agatgcaatc
     6841 aatatggggg caagcagata acaaaaaacc ccgccggagc gaggtttttt gttacatcaa
     6901 agcgagaatt atttgatttt cgcttctttg tagatcacgt gctgacgaac aactggatcg
     6961 aattttttca gttccagttt ttccggctta gtacgtttgt tcttcgtagt ggtatagaag
     7021 tgaccagtac cagcagaaga aaccagcttg attttctcac gaataccttt agccatgatt
     7081 tatttcctct aagtacttag tacttttcgc cacgggcacg cagttcagcc agaactgtat
     7141 cgatgccttt tttatcgatt acacgcatac ctttagcaga tacgcgcagg gtgacaaaac
     7201 gcttctcgct ctcaacccag aaacggtgag agtgcaggtt cggcaggaaa cggcgtttag
     7261 tcgcgttcag tgcgtgggaa cggttgttac cggtcaccgg acgcttgcca gtaacttggc
     7321 agactcggga catgtctatt ctccaaaaat caaattagct cgagcttcgt atggggtatt
     7381 ggcgcctcgt caggctttac agcccggtca tcgcagttct atgtgaactc tcgattgcca
     7441 ggcccaaatg ccaaacccga gattctcaaa ggtggcgtag tatacgctga ctcagcgatg
     7501 tgctcaagtc ccgaacagac aaagatcccg aaggatcgcg catagcgggt taaatccagc
     7561 cgcgttcggc aaaagaaaca tactctccac gcccaatcac gatatggtcg agcacgcgta
     7621 aatccatgaa ctgacaactc tttattatcc gttcagtaat gagtttatcc gctttactgg
     7681 gttcagcaca acccgaaggg tgattatgtg caaggatcag cgccgaggcg tttattttta
     7741 tcgcttcgcg gataatttcc cgaggatgga cttcaacatg gtttagcgtg ccggaaaaaa
     7801 gacggctatg cgttataacc cggtgttgtg agtcgagaaa gatcaccata aagatctccc
     7861 gctcctcacc cgtgagctgg ctttgtaaaa attcccgcgt catctccggg ctgagtaaag
     7921 ggctttctgc acgcatccgc acgttgtagt aacgccgcgc cagttcagca atccccttta
     7981 actgggcaaa tttcgctacg ccaattccat gaacaccact aaattgttca tattcagagg
     8041 ttaacaagcc ataaagagag ccgaaattct ccagcatctc ttttgccagg gttaatacat
     8101 ctttaccgcg cgttccggta cgcagaaata gcgccagcag ctcgacatcc gttaaggcgc
     8161 taataccaaa cttcagcatt ttttcgcgcg gcatcaacag ctgtgcatta tttttcacct
     8221 tcacctcctt tgtggtgctc gcatcctgtc atagccgttc cgttaaatcg acggccagtt
     8281 ttcaatcctg gaaagcgcct cgcaaagtga atctcaggtg aaggcacgac aacaaaagta
     8341 ttgtgataaa atcgccaact tctggtgtca ccctacagga aaaatcatca tgagcctggc
     8401 cggtaaaaaa atcgttctcg gcgttagcgg cggtattgct gcctataaaa tccctgaact
     8461 ggtgcgtcgt ttgcgcgatc gcggggccga cgtccgcgta gccatgaccg aagcggcaaa
     8521 agcctttatc accccactta gcttgcaggc ggtttctggg tatcccgttt ccgacagtct
     8581 gcttgacccg gcagccgaag ccgctatggg ccatattgag ctgggtaaat gggctgattt
     8641 agtgattctc gcccctgcca cggcagattt gattgcccgt gttgctgccg gaatggcgaa
     8701 tgacctggta tcgacgattt gtctggctac acctgcgcct gtagccgtgc tccccgccat
     8761 gaaccagcag atgtaccgtg ccgctgccac gcagcataat ttagaggtgc ttgcctcccg
     8821 tggtttgctc atctgggggc cagacagtgg cagtcaggct tgtggtgata tcggcccagg
     8881 gcgaatgctc gatccgttaa ccattgtgga tatggcggta gcgcattttt cgcccgtcaa
     8941 cgacctgaaa catctgaaca ttatgattac cgccggtccg acgcgtgaac cgctcgatcc
     9001 cgtgcgttat atctctaatc acagctccgg caagatgggt tttgcaatcg ccgccgccgc
     9061 tgcccgtcgt ggcgcgaacg tcacgctggt atcaggtccg gtttcactac cgacgccacc
     9121 gtttgttaaa cgtgttgatg tgatgaccgc gctggaaatg gaagccgccg tgaatgcttc
     9181 tgtacagcag caaaatattt ttatcggctg cgccgccgtg gcggattatc gcgcagctac
     9241 cgtggcccca gaaaaaatca aaaagcaggc cacgcagggt gatgaattaa caataaaaat
     9301 ggttaaaaac cccgatatcg tcgcaggcgt tgccgcacta aaagaccatc gaccctacgt
     9361 cgttggattt gccgccgaaa caaataatgt ggaagaatac gcccggcaaa aacgtatccg
     9421 taaaaacctt gatctgatct gcgcgaacga tgtttcccag ccaactcaag gatttaacag
     9481 cgacaacaac gcattacacc ttttctggca ggacggagat aaagtcttac cgcttgagcg
     9541 caaagagctc cttggccaat tattactcga cgagatcgtg acccgttatg atgaaaaaaa
     9601 tcgacgttaa gattctggac ccgcgcgttg ggaaggaatt tccgctcccg acttatgcca
     9661 cctctggctc tgccggactt gacctgcgtg cctgtctcga cgacgccgta gaactggctc
     9721 cgggtgacac tacgctggtt ccgaccgggc tggcgattca tattgccgat ccttcactgg
     9781 cggcaatgat gctgccgcgc tccggattgg gacataagca cggtatcgtg cttggtaacc
     9841 tggtaggatt gatcgattct gactatcagg gccagttgat gatttccgtt tggaaccgtg
     9901 gtcaggacag cttcaccatt caacctggcg aacgcatcgc ccagatgatt tttgttccgg
     9961 tagtacaggc tgaatttaat ctggtggaag atttcgacgc caccgaccgc ggtgaaggcg
    10021 gctttggtca ctctggtcgt cagtaacaca tacgcatccg aataacgtca taacatagcc
    10081 gcaaacattt cgtttgcggt catagcgtgg gtgccgcctg gcaagtgctt attttcaggg
    10141 gtattttgta acatggcaga aaaacaaact gcgaaaagga accgtcgcga ggaaatactt
    10201 cagtctctgg cgctgatgct ggaatccagc gatggaagcc aacgtatcac gacggcaaaa
    10261 ctggccgcct ctgtcggcgt ttccgaagcg gcactgtatc gccacttccc cagtaagacc
    10321 cgcatgttcg atagcctgat tgagtttatc gaagatagcc tgattactcg catcaacctg
    10381 attctgaaag atgagaaaga caccacagcg cgcctgcgtc tgattgtgtt gctgcttctc
    10441 ggttttggtg agcgtaatcc tggcctgacc cgcatcctca ctggtcatgc gctaatgttt
    10501 gaacaggatc gcctgcaagg gcgcatcaac cagctgttcg agcgtattga agcgcagctg
    10561 cgccaggtat tgcgtgaaaa gagaatgcgt gagggtgaag gttacaccac cgatgaaacc
    10621 ctgctggcaa gccagatcct ggccttctgt gaaggtatgc tgtcacgttt tgtccgcagc
    10681 gaatttaaat accgcccgac ggatgatttt gacgcccgct ggccgctaat tgcggcacag
    10741 ttgcagtaat atgacgccgg atgacttttc atccggcgag tttctttaaa cgccaaactc
    10801 ttcgcgatag gccttaaccg ccgccagatg ttccgccatt tccggcttct cttccaggta
    10861 agcaatcagg tctttcaggg tgatgataga gatcactttg cagttgtaat cacgctcaac
    10921 ttcctgaatc gccgaaatct cgccgcgccc ccgttcctga cgatcaagcg aaatcaacac
    10981 gccagcaagc gtcgcgccat tagcctgaat aatctccatc gattcgcgaa tcgccgttcc
    11041 ggcggtgatc acatcatcta ccagcattac gcgtccttgt aacgcgctac caaccagatt
    11101 gccgccttca ccgtggtctt ttgcctcttt gcggttaaag cagtacggca ggtcaaggtc
    11161 atgatgctcc gccagcgcca cggcggtagt ggtggcaatc gggatccctt tgtaagcagg
    11221 gccaaacagc agatcgaact caaggccgga atccaccaac gcttcagcgt aaaaacggcc
    11281 taacagtgcc agatcgcgcc cggtattaaa cagcccggcg ttgaagaaat aggggctttt
    11341 gcgcccggac ttcaacgtaa actcgccaaa ctttaacacc tgcttgccaa gcgcaaattc
    11401 aataaactgg cgctgatatg gtttcatgcc ttcgctcctc atcttacttt tctacagaca
    11461 aaaaaaggcg actcatcagt cgccttaaaa atcagtttgc cagcgccgcc ttctgcgtcg
    11521 ctacaatgga ttcgattccc cctcgggcca gagccaacaa ggtgagtagc tcttcatggg
    11581 tgaacggctc gccttctgcc gtcccctgca cttcaatgat gcgcccgtct tcggtcatca
    11641 ctacgttcat gtcggtctct gcggcagagt cttcaacgta ttccagatcg caaaccgctt
    11701 cgccgttcac aattccgaca gaaactgcgg ctaccatccc tttcatcggg ttggttttca
    11761 gcttgccgtt ttccaccagc ttctgtagcg catctgccag cgccacgcag gcacccgtaa
    11821 tcgacgcggt acgcgtgcca ccatcagcct gaagcacgtc gcagtccagc gtaatggtga
    11881 actcacccag cgctttcaaa tctactgccg cgcgaagagc acgggcgatc agacgctgga
    11941 tttccattgt gcgtccaccc tgcttacctt tcgccgcttc acgagcgtta cgggtgtggg
    12001 tagaacgtgg cagcatgccg tactctgcgg tgatccagcc ctggccctga cctttcagga
    12061 agcgcggcac gccttcttca atagaggcgg tacacaacac tttggtatcg ccaaattcga
    12121 ccagcaccga gccttctgca tgttttgtat agttacgagt cagggtaacg ggacgcacct
    12181 gattattgct acggcctgct ggacgcatat tgaaatctcc ggcttgaaac aaatgtggct
    12241 gcgcattata cggacttccg gcggttattc ctatcctgac aaggcatcga tggctataat
    12301 ccttccacct ctccttttat aaacaggaac gtctatgatc cgcagtatga ccgcctacgc
    12361 ccggcgtgaa atcaagggtg aatgggggag cgcaacctgg gaaatgcgct cggtaaacca
    12421 gcgttatctg gaaacttact ttcgtctgcc ggagcagttc cgtagccttg aacctgtcgt
    12481 tcgcgagcgt attcgttctc gcctgacgcg cggtaaagtg gaatgtaccc tgcgctatga
    12541 gccagatgtt agcgcgcaag gtgagctgat cctcaacgaa aaactggcta aacagctggt
    12601 aactgccgcg aactgggtaa aaatgcagag tgacgaaggg gaaatcaacc cggttgatat
    12661 tctacgctgg ccgggcgtga tggcagccca ggagcaggat cttgacgcca ttgccgctga
    12721 aattctcgcg gcgctggatg gtacgctgga cgactttatt gtcgcgcgcg aaaccgaagg
    12781 tcaggcactg aaagcattga tcgagcagcg tctggaaggc gtcaccgccg aagtggtcaa
    12841 agtccgctcc catatgccgg aaatcctgca atggcagcgt gaacgcttgg tcgcgaagct
    12901 ggaagatgct caggttcagt tggaaaacaa ccgtctggag caggagttgg ttctgctggc
    12961 acaacgaatt gacgttgccg aagaactgga ccgcctcgaa gcgcatgtta aagagacgta
    13021 caacattctg aagaaaaaag aagccgttgg tcgtcgtctg gactttatga tgcaggagtt
    13081 caaccgcgag tcgaacactc ttgcgtcgaa gtctatcaat gccgaagtga caaactccgc
    13141 gattgagctg aaagtgttga ttgagcagat gcgcgagcag attcagaaca ttgaataact
    13201 cgtaacgcca attcttactt ttcagccttc acaaatgccg cccactcaaa cagagcggca
    13261 tttttcttcc ccgcaacttc aattctgttt tgcgtgcctg ctccagattt tgcgatgttt
    13321 ttttgcccag cacactgaga acgtgagata ctcacaactg tatataaata cagttacaga
    13381 tttactttct ttgcaatcga tatcacatgg agtgggcaat gaacgaacat catcaacctt
    13441 ttgaagaaat acggcattat ggcactgaag ggcaagaatt ttggtcagct agagaattgg
    13501 caccgttact ggattatcgg gactggcgca attttcaaaa ggtactggcc cgcgcgactc
    13561 aagcgtgcga agccagcaat caagctgcat ctgaccattt cgttgaaacc accaaaatgg
    13621 ttgtattagg ttcaggggct caacgagaac ttgaagatgt tcatctctcc cgctatgcct
    13681 gttacctggt agtacaaaac ggcgaccctg cgaaaccggt tattgcggca gggcaaactt
    13741 attttgctat ccagacccga cggcaggagc tggctgacga tgaagctttc aggcaacttc
    13801 gtgaagacga aaagcgtctt tttctgagaa atgaattgaa agaacataac aaacaattgg
    13861 ttgaggcagc gcagcaagct ggggtagcga cagctatcga tttcgccata tttcagaatc
    13921 atggttaccg ggggctgtat ggtggattag atcagaaagc tattcatcag cggaaggggc
    13981 tgaaaaagaa tcagaagatc ctggatcata tgggttcaac agaactggcg gctaatctct
    14041 ttcgagctac ccaaacagaa gaaaaactca agcgggatgg cgtgaattca aaacagcaag
    14101 caaacaccac acactttgac gtgggtcgca aggtgaggca aaccattcag gaacttggcg
    14161 gaaccatgcc tgaggagttg ccgaccccgc aggtcagcat caagcagttg gaaaacagtg
    14221 taaaaattac agagaagaaa tagcagaaac taaaaactct ttttgttgat tgagacaccc
    14281 gatgcgtaag ccaaggtcca ggtgcagtga acacaatggc taaatattgc acctttcttt
    14341 tcccccctca gttttaacct attttttctt atgcattttc tcagacaaga agtcagaaga
    14401 atgcatctct gctacagaaa atagcgattt cacataacta caagttatca atttcccctc
    14461 accttaaaaa atctcaatcg tgacaatgcg cacaaatcgc taccctgcca gacagatttt
    14521 tagggagaga accatgctgt tacacatttt gtatttggtt ggcattactg ccgaagccat
    14581 gacgggggcg ctggcggccg gacgacggcg catggataca tttggcgtaa ttattattgc
    14641 gaccgccacc gcaattggcg gagggtcagt gcgcgatatt ctgctgggcc actatccgct
    14701 cggctgggtc aaacacccgg aatatgtgat tatcgtcgct accgccgcag tactcaccac
    14761 tatcgttgct cctgtaatgc cttacctgcg caaagtgttt ctggtgctcg atgcgctcgg
    14821 actggtggtc ttttctatca tcggggcaca ggttgcactg gatatggggc acggcccaat
    14881 tattgccgtt gtcgcggcgg taaccaccgg cgtgtttggc ggcgttttgc gcgatatgtt
    14941 ctgtaaacgc atcccactgg tattccagaa agagttgtat gccggtgtct catttgcctc
    15001 cgccgtgctg tacatcgcgc tgcaacacta tgtttctaat catgatgtgg taattatctc
    15061 caccctggta ttcggctttt ttgcccgctt actggcgcta cgccttaagc tgggattacc
    15121 ggtcttttac tacagccacg aaggacacta aggttcaaaa cctgtgatct gctgggcagc
    15181 cagccaactg cccagcttct tgatttgcgc attttccttc cattcaataa cctgtctggc
    15241 gcgccccgat ccagtgcccg gcagctgctg ccagaactgc tccgtgctaa ataaaagttg
    15301 cgaccaggac cgttcatcac tggcattaag cgccgcccgg gttagcggta ttcccattgc
    15361 catcacccag cgagtaaaag gctgcttacg agccagatta aactgatgcc atagctgcgc
    15421 acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc tctggcgtta ataaaagcca
    15481 ggaaaagata tgctcaaagc gatgagtctg atgcagcgcg cgccagccgg cctcaccaat
    15541 gccatccagc ccaagaacct gttttgcccc cagccagact aagcgtgaaa tgaactgttc
    15601 ctgacaaaca tcagaagcaa agtagcaggt caacgagtta aagcggtttt ctggcggtgt
    15661 cggttttgta cgttctgcac cgcgccacac cacatcatca atgcgaggaa taccctgacc
    15721 ggcaaggctg acgagaatct gatcaccagg cgcaatatcc cactcctgcc agcgcctgac
    15781 ggaaccaata ttcacccgct ggactttttt atcatccagc atgacaggtg cgagtgacgc
    15841 aaccaccgat attttaccgc tcttacccac cgcaaactga atcgccttca cttcggcaac
    15901 ctgagctaca ggttgatatt tccaggccac cagccactct gcctggcccg gtagccaatg
    15961 gcgggattct ggctctttcg ccgctcgtac aactacgcca tcggtgacga agggtaattc
    16021 cgctttccac cactcattgc gtacgcgcgc aacttcatca gcatttttca ccgcacgggt
    16081 atacgtctgc gttagagtaa aacctgcggt agccagctct tttaaacgat cagacattaa
    16141 ctgcggtcca tccggccatg cccagacaaa aacgcccaga gaattcagcg tgtcgctatc
    16201 gtcctggcgc atcatcaagc cagcaacttt tgcgcgggca tttattcccc ccatttgttg
    16261 ttggatatgc ccctcgcgct ggagaaatat ttccccctga agcgtactgt tggctaaagg
    16321 cccgctaacg gtttgcggca cagcggaaat taagctaact ttctgcgtcc agtcctcgcc
    16381 tttcaggcca ttaccgcgac tgattgcttt gttcagtttc ccgtcccgat aaaccagggt
    16441 taccgcaacg ccatcaactt ttggctgcac ccaaagatcg ctacgttctc gcatccacag
    16501 acttaatgcg tttttatcca ccattttgcg tacacccgta tgggcaaccg gatgcataac
    16561 cgcaccattt aaaggcggca tcatcacgtc gcggggctcg ctcccaaagc agcgttgcca
    16621 ctgcgtaaga cgggcgctta actgatcgta aacaccgtct tccacctcac tttttccttc
    16681 cttccagtag tcatcgtccc actgttttat ttgctgttgc aggcgggaaa tttcttcctg
    16741 tgctctggct ggcgaccagg ccggacagac cgcccacaca gatgattgcc agcacaagat
    16801 acttattaat atcgccatcc atactttcat catcacctcc gctgtaggta gtcaggcaga
    16861 tatacaacgt gatgaaagca aagccgagtg gcaaaaacgg agtctgcgag gacgcttcct
    16921 gaaaatcgtc tttattgcag tgaatgacag gcaaatgcgg aagcagctac gcaaaacgca
    16981 acaactttgc gcaaaaagtg tgagcaaggg ctacgtcaca tggccgcgcc gtgtataata
    17041 agctcgtatg taggctttat ttcgctaatc acatacgaaa gatactcatg gctcaaggca
    17101 cgctttatat tgtttctgcc cccagtggcg cgggtaaatc cagcctgatt caggctttat
    17161 taaaaaccca accgttgtat gacacccagg tttctgtttc acacaccaca cgccaaccgc
    17221 gtcctggtga agtccacggt gaacattatt tctttgttaa tcatgatgaa tttaaagaaa
    17281 tgattagcag agatgcgttc ctcgaacacg cagaagtttt tggtaattac tatggcactt
    17341 cgcgtgaggc cattgagcaa gtactggcga ccggtgtcga tgtttttctc gatatcgact
    17401 ggcagggcgc gcagcaaatt cgccagaaga tgccgcacgc gcggagtatc tttattttac
    17461 cgccgtctaa aattgaactg gaccgccgtc tacgcggtcg cggtcaggac agcgaagagg
    17521 tcattgcaaa gcgtatggcg caagctgttg cagaaatgag ccattacgcc gaatatgatt
    17581 acctgattgt gaatgatgac ttcgataccg cgttgaccga tttgaagacc attattcgcg
    17641 ccgaacgtct gcgcatgagc cgccaaaagc agcgtcatga cgctttaatc agcaaattgt
    17701 tggcagactg aacctgattt cagtatcatg cccagtcatt tcttcacctg tggagctttt
    17761 taagtatggc acgcgtaact gttcaggacg ctgtagagaa aattggtaac cgttttgacc
    17821 tggtactggt cgccgcgcgt cgcgctcgtc agatgcaggt aggcggaaag gatccgctgg
    17881 taccggaaga aaacgataaa accactgtaa tcgcgctgcg cgaaatcgaa gaaggtctga
    17941 tcaacaacca gatcctcgac gttcgcgaac gccaggaaca gcaagagcag gaagccgctg
    18001 aattacaagc cgttaccgct attgctgaag gtcgtcgtta atcacaaagc gggtcgccct
    18061 tgtatctgtt tgaaagcctg aatcaactga ttcaaaccta cctgccggaa gaccaaatca
    18121 agcgtctgcg gcaggcgtat ctcgttgcac gtgatgctca cgaggggcaa acacgttcaa
    18181 gcggtgaacc ctatatcacg cacccggtag cggttgcctg cattctggcc gagatgaaac
    18241 tcgactatga aacgctgatg gcggcgctgc tgcatgacgt gattgaagat actcccgcca
    18301 cctaccagga tatggaacag ctttttggta aaagcgtcgc cgagctggta gagggggtgt
    18361 cgaaacttga taaactcaag ttccgcgata agaaagaggc gcaggccgaa aactttcgca
    18421 agatgattat ggcgatggtg caggatatcc gcgtcatcct catcaaactt gccgaccgta
    18481 cccacaacat gcgcacgctg ggctcacttc gcccggacaa acgtcgccgc atcgcccgtg
    18541 aaactctcga aatttacagc ccgctggcgc accgtttagg tatccaccac attaaaaccg
    18601 aactcgaaga gctgggtttt gaggcgctgt atcccaaccg ttatcgcgta atcaaagaag
    18661 tggtgaaagc cgcgcgcggc aaccgtaaag agatgatcca gaagattctt tctgaaatcg
    18721 aagggcgttt gcaggaagcg ggaataccgt gccgcgtcag tggtcgcgaa aagcatcttt
    18781 attcgattta ctgcaaaatg gtgctcaaag agcagcgttt tcactcaatc atggacatct
    18841 acgctttccg cgtgatcgtc aatgattctg acacctgtta tcgcgtgctg ggccagatgc
    18901 acagcctgta caagccgagt ccgggccgcg tgaaagacta tatcgccatt ccaaaagcga
    18961 acggctatca gtctttgcac acctcgatga ttggcccgca cggcgtgccg gttgaggtcc
    19021 agatccgtac cgaagatatg gatcagatgg cggagatggg tgttgccgcg cactgggctt
    19081 ataaagagca cggcgaaacc agtactaccg cacaaatccg cgcccagcgc tggatgcaaa
    19141 gcctgctgga gctgcaacag agcgccggta gttcgtttga atttatcgag agcgttaaat
    19201 ccgatctctt cccggatgag atttacgttt tcacaccgga agggcgcatt gtcgagctgc
    19261 ctgccggtgc aacgcccgtc gacttcgctt atgcagtgca taccgatatc ggtcatgcct
    19321 gcgtgggcgc acgcgttgac cgccagcctt acccgctgtc gcagccgctt accagcggtc
    19381 aaaccgttga aatcattacc gcaccgggcg ctcgcccgaa tgccgcgtgg ctgaactttg
    19441 tcgttagctc aaaagcgcgc gccaaaattc gtcagttgct gaaaaacctc aagcgtgatg
    19501 attctgtaag cctgggccgt cgtctgctca accatgcttt gggtggtagc cgtaagctga
    19561 atgaaatccc gcaggaaaat attcagcgcg agctggatcg catgaagctg gcaacgcttg
    19621 acgatctgct ggctgaaatc ggccttggta acgcaatgag cgtggtggtc gcgaaaaatc
    19681 tacaacatgg ggacgcctcc attccaccgg caacccaaag tcacggacat ctgccaatta
    19741 aaggtgccga tggcgtgctg atcacctttg cgaaatgctg ccgccctatt cctggcgacc
    19801 cgattatcgc ccacgtcagc cccggtaaag gtctggtgat ccaccatgaa tcctgccgta
    19861 acatccgtgg ctaccagaaa gagccagaga agtttatggc tgtggaatgg gataaagaga
    19921 cggcgcagga attcatcacc gaaatcaagg tggagatgtt caatcatcag ggtgcgctgg
    19981 caaacctgac ggcggcaatt aacaccacga cctcgaatat tcaaagtttg aatacggaag
    20041 agaaagatgg tcgcgtctac agcgccttta ttcgtctgac cgctcgtgac cgtgtgcatc
    20101 tggcgaatat catgcgcaaa atccgcgtga tgccagacgt gattaaagtc acccgaaacc
    20161 gaaattaatg ttttatgaac ccaacacgtt atgcacgcat ctgcgaaatg ctcgccaggc
    20221 ggcagcctga cctgaccgtc tgcatggagc aggtccacaa acctcataac gtttctgcga
    20281 ttattcgtac cgcagatgcc gttggcgtac atgaagttca cgccgtctgg cctggtagcc
    20341 ggatgcgcac catggcttcg gcagcggcgg gtagtaacag ctgggtacag gtgaaaacac
    20401 accgcaccat tggcgatgcc gtcgctcatc tgaaaggcca gggcatgcag attctggcaa
    20461 cccatctttc tgataatgct gtcgatttcc gcgaaattga ttacactcgc ccgacctgca
    20521 ttttgatggg acaggagaaa acgggcatca cgcaggaagc attggcccta gcggatcagg
    20581 acatcatcat tccgatgatc ggcatggtgc agtcgctgaa tgtttccgtt gcctcagccc
    20641 tcattcttta cgaagcccag cgtcagcggc aaaatgcagg catgtacctg cgtgaaaaca
    20701 gcatgttgcc ggaagcagag caacaacgcc tgttgtttga aggcggctat ccggtgctgg
    20761 cgaaagtcgc aaaacgcaaa ggcctgcctt acccccacgt caatcagcaa ggcgagatcg
    20821 aagctgatgc tgactggtgg tccaccatgc aggccgcagg gtaagcgcca tgaaaggccg
    20881 cctgttagat actgtcccgc tcagttccct aacgggcgtt ggcgcagcgc ttagtaacaa
    20941 actggcgaaa atcaacctgc ataccgtgca ggatttactc ttacaccttc ccctgcgcta
    21001 cgaagatcgc acccatctct accccatcgg agaactactg ccgggcgttt atgccacggt
    21061 ggaaggcgaa gtgctgaact gcaatatctc cttcggcggt cggcggatga tgacctgcca
    21121 gatcagcgac ggttccggca tcctcaccat gcgctttttc aatttcagcg cggcaatgaa
    21181 aaatagcctg gcgacgggcc gccgtgtact ggcttatggc gaagcaaagc gcggtaaata
    21241 tggtgcggag atgatccacc cggaataccg cgtgcagggc gatctcagca cgccagaatt
    21301 acaggaaacg ctcacgccgg tttatccaac aacggaaggc gtaaagcagg ccacgctgcg
    21361 taaattaacc gaccaggcgc tggatctgct cgacacctgc gccattgaag aactcctgcc
    21421 gccggaactg tcacaaggaa tgatgacgct accggaagcg ttgcgcactt tgcaccgccc
    21481 gccaccgacg ctacagctta gcgatctgga aaccgggcag catccggcgc aacgtcgtct
    21541 gattctggaa gaactgctgg cgcacaacct cagcatgtta gccttacgtg ccggagcaca
    21601 gcgttttcat gcccagccgc tgagcgccaa tgacacgctg aaaaataaac tcctcgccgc
    21661 cttaccgttc aagccaacgg gcgcacaggc acgcgtagtg gcggagatcg agcatgatat
    21721 ggcgctggat gtgccgatga tgcgcctggt acagggcgat gtaggttccg gtaaaacgct
    21781 ggtcgccgcc ctcgccgcgc tgcgtgcgat tgcccacggc aaacaggtgg cactgatggc
    21841 accaaccgaa ttactcgccg agcagcacgc caataacttc cgcaactggt ttgcaccgct
    21901 cggtatcgaa gtgggctggc tcgccggtaa gcagaaaggc aaagcgcgac tgtctcagca
    21961 ggaagccatc gccagcggtc aggtgcagat gattgtcggt acacacgcca tcttccagga
    22021 acaggtgcag tttaacggcc tggcgctggt gattatcgac gaacagcatc gttttggcgt
    22081 gcatcagcgt ctggcattgt gggagaaagg ccagcagcag ggcttccatc cgcatcagtt
    22141 gatcatgacc gccacgccga tcccccgcac gctggcaatg acggcgtatg ccgatctcga
    22201 tacctcggtg atagatgagc tgccgccagg ccgcacgcca gtgactacgg tcgctattcc
    22261 tgatacccgc cgtaccgaca tcattgaccg cgtgcgccac gcctgcataa ctgaaggtcg
    22321 tcaggcgtac tgggtttgta cgttgattga agagtcagag ttactggaag cgcaggcggc
    22381 ggaagctacc tgggaagagt tgaaactggc gctaccagaa ttgaatgttg gcctggtaca
    22441 cgggcggatg aaaccagccg agaaacaggc ggtgatggcg tcgtttaaac aaggtgagct
    22501 acatctgctg gttgccacta ccgttattga agtcggtgtt gatgtgccta acgccagcct
    22561 gatgattatc gaaaaccctg agcgtctggg tctggcgcag ttacaccagc tgcgcgggcg
    22621 cgtaggtcgt ggcgcggtgg cttctcactg cgtgctgctc tacaaaacgc cgctttctaa
    22681 aacggcgcaa attcgcctgc aagtgctgcg cgacagtaac gacggttttg tgattgcgca
    22741 aaaagatctg gagattcgcg gccctggcga attgttaggc acgcgtcaga cgggtaatgc
    22801 tgaatttaaa gtggcggatt tactgcgcga tcaggcgatg atcccggaag ttcagcgcct
    22861 ggcgcgacat attcacgaac gttacccaca acaggcaaaa gccttgatag aacgctggat
    22921 gccggagaca gaacgttact cgaatgcgta aaaggcggca gtgagaagac cgccatttca
    22981 ggttacccta ccttcctgcc ggatgcgatt caccctacaa attcaataaa ttgtgaatca
    23041 atgcgcagcc ttgataagcg aagcgtatca ggcatttgtc tttgttgcgg cggcacttct
    23101 accgccgctt catcggttaa cccgcaaaaa tcggcaacat caaatacaac ttaattacca
    23161 gcgcgttaac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    23221 gcgacgggcc aaaacgttca gtgatcgcct gcatgttggc gattgccgtt ggcgttgcac
    23281 cgaggccaaa accacagtga cccgcagcca gcactgccgc atcgtagttt ttgcccatca
    23341 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    23401 ccagcatcgg cagcgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    23461 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    23521 aaaagcccat cattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    23581 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    23641 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcggccca
    23701 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    23761 tggagtgttt caccagatag cgcgctaccg gaccgccaat caagccgccc agcaccaaac
    23821 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    23881 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    23941 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    24001 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    24061 acaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    24121 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt accagtagcg
    24181 ctagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    24241 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    24301 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    24361 tcatacttca tcgcgcagtg gcaacctcat gaactatgac gggtatcgag ttatgctttc
    24421 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    24481 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    24541 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    24601 gatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    24661 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    24721 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    24781 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    24841 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    24901 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    24961 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    25021 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    25081 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    25141 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    25201 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    25261 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    25321 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    25381 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    25441 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    25501 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    25561 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    25621 gatccagttg actggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    25681 cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag aaccggttct
    25741 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    25801 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    25861 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    25921 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    25981 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctcttta
    26041 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    26101 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    26161 agtggcgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    26221 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg atcaccgttt
    26281 ttccgcgcca tctcatatcg tgctggagaa cgtcacgttt ggtcgtgatg gccagcccgc
    26341 gaccctggtg gccaaaagtg tcgacattgc gctaagcagt cggcaactga ccgaaccacg
    26401 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    26461 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    26521 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgta gttccgtggt caccaaaagc
    26581 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    26641 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    26701 gactaacctg ggtgccgaca tcgcgcgcgg gacattaacc ggaaacgcgc agcgtaacgc
    26761 cgacggcagc tggcaagtgg aaaacctgcg catggcggat atccgtctac aaagcgaaaa
    26821 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgcct
    26881 ggaagtgatc gatgctcgtt tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    26941 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caagaaggca aactgtcgat
    27001 gaacgctagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    27061 attttccccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    27121 cagaacgtca gggaactggc tgcgtgacgg gaaaacgttg atccttgatg atgcggcaat
    27181 tgccgggctg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    27241 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    27301 tatcgaccct gacttcccgt ggcagctcac cacgctcgat ggttacggtg ccaacctgac
    27361 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    27421 cgcgacattc aatcgtgttg atgttcgtcg cccgtcgctg gcgctgaccg ccaacagcag
    27481 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccactgc
    27541 cagtgtttca caaacgccac aacgtcagac ccatatcagc ctgaatggac gcggtgtgcc
    27601 ggtgaatatt ttgcaacaat ggggatggcc tgaattaccg ttgactggcg acggcaatat
    27661 tcagcttacc gccagtggag atattcaggc caatgccccg ctgaaaccta cggttagcgg
    27721 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgctggcat
    27781 cgtttccagc ggtgaagtta catcgacgga gccggtgcgg taaacatcaa tctcgccggg
    27841 tacgactcag acgtacccgg cattccatca atagattaca acgtaattgt cagcgcattc
    27901 ccttgaggct tcaccaccag cccctgctca ctttcagcct gcgaaccgtc ttgcagacca
    27961 tttactttca caacattgcg caggcacagt gtccagttct tcgcctcgcc cgcaccagtc
    28021 acagtaatcg tgttgccagt acgtgctgct tttaaagtaa agatcaccga tccgtcagca
    28081 gcgggcactt cacagacggc ttcatgcccg tcttgcagat taaagaggtg gaatgccgtg
    28141 ccttcgtgcc acacgtaatc gggacgttga tcgttgttgc ccagcgccag tagagtgtta
    28201 tcacgcacat aaacgggcag actcaggaag ccgtgctgct gtttatgcca gcgactaccg
    28261 tcgagttcat cgttgtgcca caggtgtgtc cagcgacctt ccggcaggta gaactgcaca
    28321 tcgcccgctt cagtgaacac cggcgcaacc atcacgttgt cgcctaacat gtattgacgg
    28381 tcaaggtaat cacaagccgg atcgtccggg aactccatca tcatggcccg catcatcggc
    28441 gtaccccgcg cgttcgcacg cgcagcttca cgatacagat acggcatcat gcggcatttc
    28501 agttgcgtga agaagcgcac cacatcacag gactcatcat cgtaggccca cggcacacga
    28561 taagatttgc taccgtgtaa acggctatgg ctggagagca aaccaaacgc gcaccagcgt
    28621 ttgtaaacgt gcgccggagc ggtattttca aagccgccga tatcgtggct ccagaagcca
    28681 aaacctgaaa ggccaataga caaaccaccg cgcaggcttt ccgccattga ttcgtagtta
    28741 gcgtaacaat cgccacccca gtgtaccggg aatttctgcg caccgacgga ggccgagcgg
    28801 gcaaacaaga cagcttcttc ctcaccaacg gtgtccttga gcacgttcca caccagttcg
    28861 ttgtagatgt acgcataatg gttatgcatt ttctgcggat cggaaccgtc aaaccactga
    28921 acatcagttg ggatacgttc gccaaagtcg gtcttaaagc aatcaacgcc catcgcgacc
    28981 agacctttca gtttgtcggc gtaccatttg caggcatccg gattggtaaa gtcataaatc
    29041 gccagacctg gctgccattt atcccactgc catagcgaac cgtccgggcg tttgagtaaa
    29101 tagcctttct cttgtaactc tttaaagacg ggggattttt gaccgatata ggggttaatc
    29161 cagacgcaga ttttcagccc tttcgctttc aggcggcgga tcatcccttc cgggtcaggg
    29221 aaagtcagcg ggtcccactc aaaatcgcac cactggaagg ctttcatcca gaaacagtca
    29281 aagtggaaaa catgcagcgg cagattgcgt tccgccatac catcgataaa gctgtttacc
    29341 gtcgcttcgt cgtagttggt ggtaaatgaa gtggttagcc acaggccgaa ggaccacgcg
    29401 ggcggcagcg ccggacgacc agtaaagcgg gtataacgat caagtaccgc tttcggcgtc
    29461 gggccgtcga taacaaagta ttcgagatat tcactctcaa cgctgaactg cactttggag
    29521 actttctccg atcccacttc aaaagagaca cactggggat gattgaccag taccccataa
    29581 ccacggttag tcatgtagaa cgggatattt ttatacgcct gttcagtact tgtgccgccg
    29641 tcccggttcc aggtctctac cgtctggcca ttgcgcacca gggcagtaaa gcgctctccc
    29701 agaccgtaaa ctgtttcgcc aacgccaaga tcaagccgct caaacatata attgcgttga
    29761 ttattcgtgt cctgcacgta gccattattt ttcacctgac taccggtaat acgttcgccg
    29821 ttgcgcagaa aatccagtga ccagaactca cctttgctga cacgcgcgct taagttgcca
    29881 cttttaaact cagcataacg ttctgtgttt tcgattgtga ccttcacgtc ctgcaaaata
    29941 ttgagcggat aatgaggacc gttattcagc gccccctgaa aatgctcaat ccgcacaccg
    30001 acaatacctt cctgtgggga gaaaaagcgc agcgtaaata aaggcgtatc aagctgccag
    30061 gtacgttcac gcacatcacg gggggcagca tagaccacca tttcattatc ctgctgttca
    30121 acctcgaaca cctgaagcgg gtgaatcaaa ttgaggccag gttgaatcaa ccagtttcca
    30181 tcgctaattt tcattgccgt tccttagttc tgcacttctt gagaggtgaa ttgttgctgg
    30241 caacggcgtt taccctgagc cagctgttcc ataacggttt tcagattgtg cgtcgtgagt
    30301 gagtagtagc gtttagcgat aatcgcgctc agcaaataac agatcgccgg aacaatcgtg
    30361 aatagcgcaa taatgatgct aatcgtggcg ctgttctgcg ctttttctgc cgcatcatat
    30421 ccgccataag ccagcatcca gccgataaga gcgccgccga aggccaaccc cagtttgagc
    30481 acaaacagcg tgccagcaaa actgatcccg gtcaggcgtt taccattgca ccactcgccg
    30541 tagtcgacgg tatcggacat cattacccac tggataggtg tcaccagttg atgcaacaca
    30601 ccaatcacga agatgaagac aaacatagtg atgctggcct gcatgggaac aaagaacatc
    30661 gcgaggctaa tcactgccag cagggcgttc gtccaccaga agatagtgac tttacatttc
    30721 cagtcggtca gaggttttgc cagtgcggaa ccaatcaggt tacccacgca ataagtggtg
    30781 agaaaagcga caaacacttc cggcgtgccc aaaatccatg tgacgtaata catcatcgcc
    30841 ccaccgcgta cgcacaccgc caggatattg aaaatggtta gtaaaccgac aatccgccac
    30901 tggtcgtttt gccagatatc acgtaaatct tcccgcatag acgttgttgt aggtggtgct
    30961 tcaacgcgtt ctttagtggt gaagaaacaa aatgccagca tcatgaatgc caccacggaa
    31021 aggaccgcga taccgccctg gaaaccgagt ggtttattat caccgccaat taaattaacc
    31081 agtggcatca tcagaacagt agaaagcatg cctcccgcgg tcgccagcac aaaacgccaa
    31141 gattgcagcg agatacgctg agtcgggtca ttggtgatta caccacccaa tgcgcagtaa
    31201 gggatattga cgacggtata aagtaaggta agtagggtgt aagtaattgc tgcatagatc
    31261 attttgccgt tcatactgag atctggcgtg ctataggcca gtacacagac gatcccgaat
    31321 ggcagtgcgc caaacagtac ccacggacga aatttacccc agcgagagcg cgttcgatcg
    31381 gccaacaacc ccatgcaagg atcggaaatc gcatccagtg cacgagcgac caaaaacatg
    31441 gttccgacaa atccggcagg aatgccaaaa atatcggtat aaaagaacat catatataac
    31501 attacgttat cgaaaataat gtggctggcg gcgtctccca tgccataacc aattttctct
    31561 ttaacggaca acacttcact cttcatctgc ttttccttga tgctataggg gtgctgagac
    31621 cgataacgca ttttgtaaac catccctgtg agagcgaata ttccgatatc tggttattaa
    31681 tttatgattc ttgttttatg tgatcgtggt agcgttaatt ccgctcatat atcattgtaa
    31741 aatatgggtt ttatatgaac tataatgctt tcgtgataat acgctgcgtg tattaggcgg
    31801 aaaaaactga tctgggggat gtagaaactc aaggaagtag ctataatgcg ccccgcctcc
    31861 atgtagcaat cgaggcgcgg aagatcgtcg tctccggtga ggcggctgga cttcaaatcc
    31921 agttggggcc gccagcggtc ccgggcaggt tcgactcctg tgatcttccg ccaaacctca
    31981 tccagccagt tccgagatac tccataaaat actttatatt caatacgatg aataatcagc
    32041 ttgtccgtaa tagtccgaga tcatctccct gaatccgcga aaaatatgta tagtaatgtg
    32101 tatagatttt ttatacgtag ttttcagtta tacacatggc tctaaccgat atacagatca
    32161 aacgtgcaaa accacaagac aaaccataca cattgaacga tggacaaggt ctgtcattgc
    32221 ttatcaatcc cgatggctcg aaaggctggc gtttccgttt ccggtttgca gggaaagcgc
    32281 ggttaatgtc atttggcagc tacgatttag taagcctcgc agaagcacgt gagaagcgtg
    32341 atatcgcccg taagcaggtt gctaatggca ttgacccggt agaggaacgc aaagctttaa
    32401 gactcgctca aaagctatca acagaaaatt ctttcgaagc aatatgtcga gaatggtata
    32461 ccaacaaagc tgatcgctgg actgtagcct atcgagaaga aatcattagg acattcgagc
    32521 aggatgtctt cccgtttatt ggtaaacgtc ctatcagtga aatcaaacca ttagaactgc
    32581 ttgaagtatt gcgacgaata gagaaacgag gagcactaga gaagaccaga aaagtgcgcc
    32641 aaagatgcgg tgaggtcttt cgttatgcaa tcataactgg ccgagctgaa tacaacccag
    32701 cacctgattt agctatcgct ctggccgttc ctaagccaaa acaccaccca tttttatctg
    32761 tcgaagagtt gccttatttt attcgggatt tggaggcata ctccggcagc ataatcacca
    32821 aaaatgcaac taagatagtt atgcttactg gtgtaagaac gcaggagatg cgctttgcta
    32881 cgtgggaaga ggttgatttg gataaggagg tttgggagat accagcggaa cgtatgaaaa
    32941 tgcgtaggcc tcacattgtt cctttatcta ctcaggtatc tggcctacat ggaggcaaac
    33001 ggctaccgga atgtactcag tctgaaaatg ttcgggctgg ggctgccggt gatgctgaag
    33061 gaatacggac tgaattacga gaagcgccat accaaacagg ggatacagac caacctgacg
    33121 ctgaaagagg aaagctacgg cgactggctg ccaaaatgtg acgaccctgc aacaacctga
    33181 ccctcatgac ccacctgacc ggcatctgcc ggtctttttt tatcccgaca tcccccgcag
    33241 gtgaacaatc cactgttcac ccttcaccgt atattcaccc gttatcacac tgaaattaaa
    33301 agagaaaaac gaaaggtgaa cagtgtgaac aatcaaatca aaaaaaactt ttttctccct
    33361 gtgtgatttc agtgcggagg attaatcccc tgcacaagtc acatcggcag aatgccggag
    33421 gtgaagaatc gaatgttcac ccttcaccca tt
//
LOCUS       DAOFQD010000047        33256 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.047, whole genome shotgun sequence.
ACCESSION   DAOFQD010000047 DAOFQD010000000
VERSION     DAOFQD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.047"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(116..256)
                     /locus_tag="QBF46_002221"
     CDS             complement(116..256)
                     /locus_tag="QBF46_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674397.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            352..1068
                     /gene="arcA"
                     /locus_tag="QBF46_002222"
     CDS             352..1068
                     /gene="arcA"
                     /locus_tag="QBF46_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDQ6674398.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1128..2480)
                     /gene="creD"
                     /locus_tag="QBF46_002223"
     CDS             complement(1128..2480)
                     /gene="creD"
                     /locus_tag="QBF46_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDQ6674399.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(2538..3962)
                     /gene="creC"
                     /locus_tag="QBF46_002224"
     CDS             complement(2538..3962)
                     /gene="creC"
                     /locus_tag="QBF46_002224"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDQ6674400.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3962..4651)
                     /gene="creB"
                     /locus_tag="QBF46_002225"
     CDS             complement(3962..4651)
                     /gene="creB"
                     /locus_tag="QBF46_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDQ6674401.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4664..5137)
                     /gene="creA"
                     /locus_tag="QBF46_002226"
     CDS             complement(4664..5137)
                     /gene="creA"
                     /locus_tag="QBF46_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDQ6674402.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5348..6217
                     /gene="robA"
                     /locus_tag="QBF46_002227"
     CDS             5348..6217
                     /gene="robA"
                     /locus_tag="QBF46_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDQ6674403.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6214..6861)
                     /gene="gpmB"
                     /locus_tag="QBF46_002228"
     CDS             complement(6214..6861)
                     /gene="gpmB"
                     /locus_tag="QBF46_002228"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDQ6674404.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6913..7425
                     /gene="yjjX"
                     /locus_tag="QBF46_002229"
     CDS             6913..7425
                     /gene="yjjX"
                     /locus_tag="QBF46_002229"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDQ6674405.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7462..7788)
                     /gene="trpR"
                     /locus_tag="QBF46_002230"
     CDS             complement(7462..7788)
                     /gene="trpR"
                     /locus_tag="QBF46_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDQ6674406.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7878..9815)
                     /gene="sltY"
                     /locus_tag="QBF46_002231"
     CDS             complement(7878..9815)
                     /gene="sltY"
                     /locus_tag="QBF46_002231"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDQ6674407.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10026..11693
                     /gene="ettA"
                     /locus_tag="QBF46_002232"
     CDS             10026..11693
                     /gene="ettA"
                     /locus_tag="QBF46_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDQ6674408.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11800..11967)
                     /locus_tag="QBF46_002233"
     CDS             complement(11800..11967)
                     /locus_tag="QBF46_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674409.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARMTRYEIADAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12001..13233)
                     /gene="nadR"
                     /locus_tag="QBF46_002234"
     CDS             complement(12001..13233)
                     /gene="nadR"
                     /locus_tag="QBF46_002234"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDQ6674410.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(13254..14636)
                     /gene="radA"
                     /locus_tag="QBF46_002235"
     CDS             complement(13254..14636)
                     /gene="radA"
                     /locus_tag="QBF46_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDQ6674411.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKMRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKEPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14685..15653)
                     /gene="serB"
                     /locus_tag="QBF46_002236"
     CDS             complement(14685..15653)
                     /gene="serB"
                     /locus_tag="QBF46_002236"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDQ6674412.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            15759..16403
                     /locus_tag="QBF46_002237"
     CDS             15759..16403
                     /locus_tag="QBF46_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDQ6674413.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16431..17447
                     /gene="lplA"
                     /locus_tag="QBF46_002238"
     CDS             16431..17447
                     /gene="lplA"
                     /locus_tag="QBF46_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDQ6674414.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17479..17628
                     /locus_tag="QBF46_002239"
     CDS             17479..17628
                     /locus_tag="QBF46_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674415.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(17903..18622)
                     /gene="deoD"
                     /locus_tag="QBF46_002240"
     CDS             complement(17903..18622)
                     /gene="deoD"
                     /locus_tag="QBF46_002240"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDQ6674416.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18702..19925)
                     /gene="deoB"
                     /locus_tag="QBF46_002241"
     CDS             complement(18702..19925)
                     /gene="deoB"
                     /locus_tag="QBF46_002241"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDQ6674417.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(19977..21299)
                     /gene="deoA"
                     /locus_tag="QBF46_002242"
     CDS             complement(19977..21299)
                     /gene="deoA"
                     /locus_tag="QBF46_002242"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDQ6674418.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21426..22205)
                     /gene="deoC"
                     /locus_tag="QBF46_002243"
     CDS             complement(21426..22205)
                     /gene="deoC"
                     /locus_tag="QBF46_002243"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDQ6674419.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22463..24013
                     /locus_tag="QBF46_002244"
     CDS             22463..24013
                     /locus_tag="QBF46_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDQ6674420.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            23985..24848
                     /locus_tag="QBF46_002245"
     CDS             23985..24848
                     /locus_tag="QBF46_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDQ6674421.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNHCGECVPQCPHQALQIVDGKVVWNAAVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VGRLIFPALYL"
     gene            complement(25365..26147)
                     /locus_tag="QBF46_002246"
     CDS             complement(25365..26147)
                     /locus_tag="QBF46_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ6674422.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(26144..27217)
                     /locus_tag="QBF46_002247"
     CDS             complement(26144..27217)
                     /locus_tag="QBF46_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDQ6674423.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRVPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27339..27500)
                     /locus_tag="QBF46_002248"
     CDS             complement(27339..27500)
                     /locus_tag="QBF46_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDQ6674424.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27627..28232)
                     /gene="osmY"
                     /locus_tag="QBF46_002249"
     CDS             complement(27627..28232)
                     /gene="osmY"
                     /locus_tag="QBF46_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDQ6674425.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28625..30214)
                     /gene="prfC"
                     /locus_tag="QBF46_002250"
     CDS             complement(28625..30214)
                     /gene="prfC"
                     /locus_tag="QBF46_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDQ6674426.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30305..30982)
                     /gene="yjjG"
                     /locus_tag="QBF46_002251"
     CDS             complement(30305..30982)
                     /gene="yjjG"
                     /locus_tag="QBF46_002251"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDQ6674427.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30982..31443)
                     /gene="rimI"
                     /locus_tag="QBF46_002252"
     CDS             complement(30982..31443)
                     /gene="rimI"
                     /locus_tag="QBF46_002252"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDQ6674428.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISMQYKVE"
     gene            complement(31412..31825)
                     /gene="holD"
                     /locus_tag="QBF46_002253"
     CDS             complement(31412..31825)
                     /gene="holD"
                     /locus_tag="QBF46_002253"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDQ6674429.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31928..32959
                     /gene="rsmC"
                     /locus_tag="QBF46_002254"
     CDS             31928..32959
                     /gene="rsmC"
                     /locus_tag="QBF46_002254"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDQ6674430.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         7800 a         9166 c         8523 g         7767 t
ORIGIN      
        1 acgaaagcta gcatttagat acgatgattt catcaaactg ttaacgtgct acaattgaac
       61 ttgatatatg tcaacgaagc gtagttttat tgggtgtccg gcccctctta gcctgttatg
      121 ttgctgttaa aatggttagg atgacagccg tttttgacac tgtcgggtcc tgagggaaag
      181 tacccacgac caagctaatg atgttgttga cgttgatgga aagtgcatca agaacgcaat
      241 tacgtacttt agtcatgtta cgccgatcat gttaatttgc agcatgcatc aggcaggtca
      301 gggacttttg tacttcctgt ttcgatttag ttggcaattt aggtagcaaa catgcagacc
      361 ccgcacattc ttatcgttga agacgagttg gtaacacgca acacgttgaa aagtattttc
      421 gaagcggaag gctatgatgt tttcgaagcg acagatggcg cggaaatgca tcagatcctc
      481 tctgaatatg acatcaacct ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt
      541 ctgttagcgc gtgaactgcg cgagcaggcg aatgttgcgt tgatgttcct gactggccgt
      601 gacaacgaag tcgataaaat tctcggcctc gaaatcggtg cagatgacta catcaccaaa
      661 ccgttcaacc cgcgtgaact gacgattcgt gcacgcaacc tgctgtcccg taccatgaat
      721 ctgggtactg tcagcgaaga acgtcgtagc gttgaaagct acaagttcaa tggttgggaa
      781 ctggacatca acagccgttc gttgatcggc cctgatggcg agcagtacaa gctgccgcgc
      841 agcgagttcc gcgccatgct tcacttctgt gaaaacccag gcaaaattca gtctcgtgct
      901 gaactgctga agaaaatgac cggccgtgag ctgaaaccac acgaccgtac tgtagacgtg
      961 acgatccgcc gtattcgtaa acatttcgaa tctacgccgg atacgccgga aatcatcgcc
     1021 accatccacg gtgaaggtta tcgcttctgt ggtgatctgg aagattaatc ggctttacca
     1081 ccgtcaaaaa aaacggcgct ttttagcgcc gtttttattt ttcaacctta tttccagata
     1141 cgtaattgat cgtccattgt aacttcttta ctggctttca ttttcggcag tgaaaacgca
     1201 taccagtcga tattacgagt cacaaacatc atgccggcca gcgccaccac cagcacactg
     1261 gttcccaaca acagcgcgct atcggcagag ttgagcagtc cccacatcac accatccagc
     1321 aacaacagcg cgagggtaaa caacatgctg ttgcgccagc ctttcaatac cgcctgcaaa
     1381 taaataccgt tcattaacgc cccaatcaga ctggcgatta tccatgccac ggtaaaaccg
     1441 atatgttcag aaagcgccag caagagcaaa taaaacatca ccaatgaaag ccccaccagc
     1501 aaatattgca ttgggtgtaa acgttgcgcg gtgagcgttt caaaaacaaa gaacgccata
     1561 aaagtcagtg caatcagcag aatggcgtac ttagtcgccc ggtccgttaa ttggtattga
     1621 tcggctggcg tcgtcactgc gacgctaaac gcggggaagt tttcccagcc ggtatcattg
     1681 cctgaagcaa aacgctcacc gagattatta gcaaaccagc tgctttgcca ttgcgcctga
     1741 aaacctgact cgctaacttc ccgtttggct ggcaaaaaat cacctaaaaa actgggatgc
     1801 ggccagttgc tggttaaggt catttcgcta ttacgcccgg caggcaccac agaaagatcg
     1861 ccggtaccgc ttaaattcag ggccatattc agcttcaggt tctgcttccg ccagtcccct
     1921 tcgggtaaag ggatatgcac gccctgcccg ccttgctcta acccggtgcc gggttcaatg
     1981 gtcagcgccg ttccgttaac ttcaggcgct ttcaccacac caataccacg cgcatccccg
     2041 acgctaatca caataaaggg cttgcctaag atgatatttg gcgcgttgag ctcgctaaga
     2101 cgcgaaacat cgaaatcggc ttttagcgtt aaatcactgt gccagacctg accggtataa
     2161 atccctatct tgcgttcttc cacgttctga ttgccatcaa ccatcaatga ctcaggtaac
     2221 caaaaatgga taaaacttcg tttccgctcc acggttttat cctcttcctg caccgtataa
     2281 agctcggtca caggaatagc gataagcggc ccaacgagtt tttgcggccc gctggtactt
     2341 tggcgaatcg catcttccac atcgctacgg taatcggcac gttcgacaat cacttgccga
     2401 atcagcatta tcggaatcaa caacagcaat actgcaccaa acaaggtggt cattttccag
     2461 aacagggggg atttcaacat agtcttctcc tttgcaatgg cagcaggata cgaaggctat
     2521 gtgggaagaa tttgaagcta tgtgaagtga cggtgaagtc gaagcgaggc cagcacgcca
     2581 ccttcctgca cgttgcgcag cgtgacttcg ccgttaaaca aacgggcgac ctcactgacg
     2641 aacgccaacc ccagaccgct gcttttttgc ccatttgcac gcggcaaaga gtaaaagcgt
     2701 tcaaaaatac gtgacagcgc gtagtcagga ataccactac cggtatccag caccttaagg
     2761 gcgacgtgtt cctgatccac ttcggcgctt agcgttatgc gaccgctctc gggggtaaaa
     2821 tcgatggcgt tatccagtaa attccccagc gcctgctcca gtaacgccgg ttcagcagca
     2881 acattaacct cagtgggcat aacatgtaga gtgatgtttt tttctgccaa ctgcacggtg
     2941 cgcgcttcgc tgacgcggcg aaataatgcc gccacatcaa caacagtcag aacgacttcc
     3001 tgacgattct ccagtcttgc ctggcgtagt aacgtttcta ccaatgcctg catacgcgca
     3061 ttttgcgtca gaatgttgtc ggtaaaacga gccaccactt ccggtggcgg accttcgcgt
     3121 aaaatttccg ccgcgccacg aatcgccgcc agtgggcttt ttagctcatg ggttaacgca
     3181 taaacatact gctcaatata gtttttccct tccagcttca cgcgcatact ttccagcgcc
     3241 tgcgcaagct tacgcaactc gctactgccg agttcaggaa gaggaacggg cttattgtca
     3301 gtgacggaat cggcatagcg agtgagccgg gcaatggagc ggttgatcca ccaaaccatg
     3361 cctgcgccaa tcaccagtgc aatccccagc aaaatggcgc tggcccataa aattcgccgc
     3421 tcgctacgct taatgaccgg agccatcgcc gcgttcggtt tgcctacgct caaaacgcca
     3481 ataagccgcg agccgtccat aatcggcgcg gcaacataca tcaccgaact ttcgggatcg
     3541 gcaggatttt gcaacgtgct gcgcgcacca tactgaccac gcaacgttag ccagacgtca
     3601 ttccagcgcg aataatcctg cccaacggct ttatttgccg aatcgaacaa tactttaccc
     3661 tgcgaatcgg tcatatagac atgatattca ttgcgcactt tgttaatgcc accgatattg
     3721 gcgcgaaacg ggcgatgttg tagctgatta aacgcctgcg ccagttgccc atgcgttggg
     3781 tccccagaga gcaaatcggg acgcgccagc tccgccagca acgttgcggt atcgattaac
     3841 gtcccctccg ttgctcttcg cacgcccggt ttaacttctt tgacaaaaat agccagtacg
     3901 aaccaggccg ccaccgccac cagtaaaaaa tagcccagca gcaaccgcat gccgatacgc
     3961 attacaggcc cctcaggcta tatcccatgc cgcgatgagt attaatcggt gaaagatcgg
     4021 ggttgatggc gcgcagcttg gcacgcaacg ttttaatgtg ggtatcaacg gtgcgatcgt
     4081 aggtgtcctg cgcatcttcc cacacgctat ccattagttg ctggcgggac cagacgcggc
     4141 ctggcgactt gagtaacgtc ttcagcaata aaaactcata ccgcgtcagc gttaatggcg
     4201 tgtcaaacca gctgatctgc gccgcgggtt cattcaattc aaaatgacca atacggatga
     4261 cgggagacgg cgacgagaac ttcttcaccc gacgcagtaa ggtgcgcacc ctggcgcaca
     4321 cttcgcgggg tgaaaacggt ttagccacat agtcgtcagc gccaatttcc agcccaagca
     4381 ggcgatcgac ctcttcactt cgggccgtca ggaacagtac aggtaacgcc ggatggagcg
     4441 ccagtaactg gcggcataat tcaaagccgc tgatatcagg caaaccaaca tcgagaatca
     4501 tgacgtcggg agcctgctgg cgagctttat ccagcaccgg caagcctcgc tcaaagacct
     4561 cgacggcaaa accttcctgc tgcaacatgt agaccagcgt gtcggctatc ccttgctcat
     4621 cttccactaa ccagaccgtt tcccgttgca taaatcgcct ctgttattgc cgccacggca
     4681 tgacaggaac cgcgctaatc gcgtttttcg gcgaaccttc tacaactttg tcggagtaag
     4741 ccagataagc gagcgcgttg cgtttggcgt cataaaagcg cacgacctgt aacgacttaa
     4801 agaccaggga cgtgcgtttt ttgaatacta cctcgccctg agctttgccg tttttaatac
     4861 gatccgacag ttcaatcggc ccgacttgct gacaagaaat agccgcatcg gaggtatctt
     4921 ccgccagacc caatccccct ttaataccac ctgttttcgc ccggctcaca taacaggtga
     4981 catttttcac atcgggatcg tcaaaggctt ccacaacaat tttgtgatcc gggccgatca
     5041 ttttaaatac ggtgtcgacc gaaccaatct cttctgcatg agccaatggc cccagcatta
     5101 tcaggcttaa agacaggatc aaatgcttgt atttcatatt gttaccattt ttatctatta
     5161 cacgagcaat tagttcgtca cggtaaataa catattgatt aagaacaaaa tctcaatact
     5221 tttatttccg tcaagaccta aaacatactc tactaagaaa aaaacactga atgctaaaac
     5281 agcaaaaaat gctattatcc aattacctga tgtcaggtgc tcgttgttga aaggatgagg
     5341 atattttatg gatcaggccg gcattattcg cgacctttta atctggctgg aaggtcatct
     5401 ggatcagccc ctgtcgctcg acaatgtagc ggcgaaagca ggttattcca agtggcactt
     5461 acagagaatg tttaaagatg tcactggcca tgctattggc gcgtatattc gtgctcgtcg
     5521 tttgtcgaaa tcggcggtcg cactacgcct gactgcgcgt ccgattctgg acatcgcgct
     5581 gcaataccgc ttcgactctc aacagacatt tacccgcgca ttcaagaagc agtttgccca
     5641 gactcctgca ctttaccgcc gttctcctga atggagcgcc tttggtattc gcccgccgct
     5701 gcgtctgggt gaattcacta tgccagagca taaatttgtc accctggaag atacgccgct
     5761 gattggtgtt acccagagct actcctgttc gctggagcaa atctctgatt tccgccatga
     5821 aatgcgttat cagttctggc acgattttct cggcaacgcg ccgaccattc cgccagtgct
     5881 ctacggcctg aatgaaacgc gtccgagtca ggataaagac gacgaacaag aggtattcta
     5941 taccaccgcg ttagcccagg atcaggcaga tggctatgta ctgacggggc atccggtgat
     6001 gctgcagggc ggcgaatatg tgatgtttac ctatgaaggt ctgggaaccg gcgtgcagga
     6061 gtttatcctg acggtatacg gaacgtgcat gccaatgctc aacctgacgc gccgtaaagg
     6121 tcaggatatt gagcgatact acccggcaga agacgccaaa gcgggagatc gcccaattaa
     6181 tctgcgctgt gaactgctga ttcctatccg ccgttaacgc tgcagctcat ctaatgcagg
     6241 ggcgtccaga tgcgagatgt cccctgcggt ttcaacgacc cagccagacg ccagccacag
     6301 gctttcctga taatccacgc gcgaaataga acagttacgc agacgtaagc ggcgctctgc
     6361 ccatgctggt aatccgagaa tcgtactcac caggcatccc agtgcaatac catgactcac
     6421 cagcagcggt cggcttccct gcggtaagtc ccggcaagat tccagtgccg cattgacgcg
     6481 atcgctgagc tcctgcattg actcgccttc aggaatacgc ccgtcaacgg tgccattgac
     6541 cagctgccga cgccagttct cttcttcttc ggtcagagaa tcgatatgtc ttttttccag
     6601 cacacccatg tttaattcac gcaggcgaga atcaaagatg atgtcacagc cgcaggcctg
     6661 ggcgatgatt tccgccgtac gccgggtgcg ccctaaatcg ctgctgatga tatgcgtaat
     6721 gccaagctct ttggcacgcg tcgccacctg catcgcctgt tgctcacctt tggcggtcag
     6781 cgggctgtca gactggccct gaatacgtcg ctcggcgttc cactgcgttt caccgtggcg
     6841 gactaggtat acctgtaaca tacttttttt ccgtatactg ctgtaacgtt aattttctga
     6901 gtaatgctga ttatgcacca agttgtctgt gcgaccacca atcccgctaa aattcaggcc
     6961 attctgcagg catttcacga gatcttcggc gaaggatcct gccatattgc atccgttgcc
     7021 gtcgagagcg gtgtaccgga acaacccttt ggcagtgagg aaacgcgcgc tggcgcacga
     7081 aatcgggtag caaatgcccg ccgtttactt ccagaggctg atttttgggt ggcgattgaa
     7141 gctggcattg atggcgacag cactttcagt tgggtggtga ttgaaaacgc cagccagcgc
     7201 ggcgaggcgc gttcggctac tttgccgttg cctgcggtga ttctggaaaa agtgcgtgaa
     7261 ggtgaagcgc ttggtcccgt gatgtcgcgt tataccggta ttgatgagat tggtcgtaag
     7321 gaaggggcga tcggcgtgtt taccgccggg aaactcactc gcgccagcgt ctatcaccag
     7381 gcggtgatcc tggcgctgag tccgtttcat aatgccgtgt attaagcgcc tgatgcgcca
     7441 cgtcttatca ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactggcg
     7501 cagctccacg ggcgcggctt tcaagctgtt agatccacgc gtaatcgtcg cgatgcccgc
     7561 gccgagttca ttttttaact cacgctggct catttcgccg cgcaacagct cttcgacaat
     7621 acgcacgcga gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg
     7681 taaatggaga tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg
     7741 gtgacgctgt tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca
     7801 ttgttagcgg gggaagcaaa atgcctcccc cggttgtact cgctaaagag tacgatagca
     7861 tatcataaac gtgtggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt
     7921 ttatccccca tgaaatagcg gtagtaagcg tcataagcca gcacgttctt cacataaccg
     7981 cgcgtctcgg agaacggaat actctcgaca aatgccacag cgtcgatacg cccggcgctg
     8041 ttgccaagcc aggttcgcac ccgtccaggt ccggcgttat aagctgctga ggagaaaata
     8101 cggttattgc caaactgctg ataaacatat tgcagataac tggtgccaat gttgatgttc
     8161 gtttccggat ccagcaattg cccagggctg ctataaccag gaatagagaa catcttcacc
     8221 gtatgggtcg ctgtaccagg cataatctgc atcaggccgc tggcccctac cggtgatttc
     8281 actttcggat tccaggcgct ctcctgacga gcaatcgcca tcgcatagct ttgcgggatc
     8341 tccttcccac tggtgtaacg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc
     8401 agatgatccc acagcttccc ggcgatcgtt gcctgaacgc taagatccca ccattggttg
     8461 ttgaaagcat accgcgccag ttgagcctgc tctgtttttg acttgctctt caccagattg
     8521 gcccactcgc tacgcgcggt gttatcgaga ttccagtaca tcaactcgcg cacgcgcgcc
     8581 atctccggcc cctgagtcag ggcgctgtca acattctgcg gcgctttatc aatcttcagc
     8641 tcatactctt caccgatgcg ttgtgctgca accatcgggt agaaaccacg ctgttgcata
     8701 agttgatgca aaatctcttt cgcttcagct tcacgtccgc gttccagcag taaatccgcc
     8761 tgccagtaac gccattcatc tttctctttc gcctccatcg gcaaacgcgc cagccaggta
     8821 ttcaggccgc gacgatcgcc ggtgccaagc gccattcgca cacggcgttc aatcaacgac
     8881 gtggattgcg agcgcataat ggcgtcatcg cgccatttcg cctgctcgtc ggtgacatcg
     8941 ttgcccatca aacgccaggc gacgatatcg cgcagctcct gaatctgatc ttcattaagc
     9001 tgctgcgcct gggcaagcga cgggatcatc agccgtgcat tttcagcatc ctgccgcgcc
     9061 acactggcaa acgccaccgc tgccatttga cgggtaaaat cggtcgctcc ggttgtacgc
     9121 gcgaaggtca gtaccgtatt agggttgttc gccagtgaaa tgattgccga ggcgatagtc
     9181 tggtaatcgg caggcatctg ccctgccagc actgttacca ggccagtgtt acccgctttc
     9241 atcgccagac ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag
     9301 acgctaaata acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc
     9361 gccccttgcc aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat
     9421 tgcgcttcgg tagttccggg cttttccggg ctaaaggcta acaagccacg ccagtcttca
     9481 cgccgcgcca gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg
     9541 ttagcgcgaa caaagttagt gaccgtcacc gccggttgat tcatcagatc atcggtgatc
     9601 tggcggtatt ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg
     9661 accacatcca tttgtcgatt atcccaggcc tgcttgattt gcgcgtaacg gctacgctgc
     9721 tcatccagtg agtcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc
     9781 aacagccgcc aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac
     9841 atcaatgcag cgtcatgccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc
     9901 acgttacgga cactttttta cttttactgc gaaggtgatc gaccaactgg gattaggcgg
     9961 ggaaaaaggc tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt
    10021 ccaacgtggc tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac
    10081 gtcatatttt gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg
    10141 gtctgaacgg cgcgggtaag tctaccctgc tgcgcattat ggcgggcatt gataaagaca
    10201 tcgaaggtga agcgcgtccg cagccagaca tcaagattgg ttacctgccg caggaaccgc
    10261 agctgaaccc ggaacacacc gtgcgtgagt ccattgaaga agcggtttct gaagtggtta
    10321 acgccctgaa acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg
    10381 acaagctggc cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata
    10441 acctgaacgt acagctggag cgtgcggcag atgcgctacg tctgccggac tgggacgcga
    10501 aaatcgctaa cctctccggt ggtgagcgtc gtcgcgtagc gttgtgccgc ctgctgctgg
    10561 aaaaaccaga catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg
    10621 cctggctgga acgcttcctg cacgacttcg agggcaccgt ggtggcgatt acccacgacc
    10681 gttacttcct cgataacgtt gcaggctgga tcctcgaact tgaccgcggt gaaggtattc
    10741 cgtgggaagg caactactcc tcctggctgg agcagaaaga tcagcgcctg gcgcaggaag
    10801 cttcacaaga agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg
    10861 gaactaaagg ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa gagctgaaca
    10921 gcaccgaata tcagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc
    10981 tgggcgataa agtgctggaa gtcagcaacc tgcgtaaatc ctacggtgat cgcctgctga
    11041 ttgatgacct gagcttctcg atcccaaaag gggcaatcgt cgggatcatc ggtccaaacg
    11101 gcgcgggtaa atcgaccctg ttccgtatga tctctggtca ggaacagccg gacagcggca
    11161 ccatcacttt aggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg
    11221 ataacagcaa aaccgtttgg gaagaagttt ccggcgggct ggatattatg aagatcggca
    11281 acaccgagat gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg
    11341 gtaaacgcgt tggtgaactt tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc
    11401 tgcaggttgg cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa
    11461 ccctgcgcgc gctggaaaac gccctgctgg agttcccggg ctgtgcgatg gttatctcgc
    11521 acgaccgttg gttcctcgac cgtatcgcca cgcacatcct ggactaccag gatgaaggta
    11581 aagttgagtt cttcgaaggt aactttactg agtacgaaga gtacaagaaa cgcacgctgg
    11641 gcgcagacgc actggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa
    11701 aatgccggat gcggcgtgaa cgccttatcc ggcctataaa ccaagaaatt tcaatacgtt
    11761 gtgaatctcc aggcctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg
    11821 ccggatgcgg cgtgaacacc ttttccggtc tacaacagcc ttagcatccg caatttcgta
    11881 gcgggtcatc cgtgctggtt ctttcaaacc ttgtttaatc tcgacagcct cttccagaga
    11941 ggttttcaac tcatcaaaaa aactcatttt atacctcgcc tttgagcagt ttcgtcacgg
    12001 ttatccctgc tcccccatca tctcccgcac cagctccacg cagcgaagga aacggctgtc
    12061 ataatcgtcc tcttcaaccc gcacgaattc gatattgttc tcttccagca tctccaccag
    12121 caagttctgg aactctttgc gatccaccga actgccgagg ctgcgtaaac catccgccac
    12181 ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa cggtattcat caatcagcgc
    12241 ctgcacgaac ggatgctcac gcccttcgta ctttttgcag aacgcctggg tagtgacaaa
    12301 atcagtgtca ataaacgcca ctttattggc atatttcact gcaaaatcaa tgtattgagc
    12361 gtggcccagc gcgattttat cgtagtcgga atactgcaat gcgatctcat caccgccgag
    12421 gtgtgaaaag acataatcgc gaccatattc ccacgcactg gtggtattga agatattagc
    12481 aagtttgttt accagggtgg atttaccgct cgactcgccg ccaaggatcg ccacggtacg
    12541 cacaaagaac ggcttcactt ccgtaggaat atattcccag tagcggaacg ggttttcgcg
    12601 gatctgtgca ccgctgatac tcataaaggt acgtttcgga tctaccagca ccgtttcgat
    12661 ccccagatgt tccatatact gtggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg
    12721 ctgaatcccc ttttcagcca taaacttttt gatgccgttg ctccacacat cccagccgtg
    12781 cggatacggc tccatgccct cttcgttgaa agcatgaatg cgaatatttt tctgatattt
    12841 aaaagtttgt aataaccagc gcagacgatc cggcaccgtg ggctgctgcg acatggcgct
    12901 gtcttcaaac aatgcgcggt cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc
    12961 aacctggcta caggcgcgct ggataaggta gatatgtccg gtatgcagtg gatagaactt
    13021 accgaatacg acaccgatcg ttttcttctg ccggggaaac tcaagcccca aaaaacggtg
    13081 caacgcctcc agcttttgcg cgctggggct tttaattttg gcattcagta actggcttaa
    13141 ataccctttg gtcataccgc tggcatcagc tacctgctgt agcgtgcagc cctgttgctt
    13201 gatggcagtt ttcaggtaat caaatgacga catatctccc tccgtatctc tcattataag
    13261 tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc caaaaatctg catcccttcc
    13321 ggctcttttt tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc
    13381 gcttcagaga ttcgttcctg accgctgggc accgggcgga tctcccctgc cagcccgact
    13441 tcaccaaaca ccaccagatc ctgcggcagc ggtctgtcgc gcaggctgga aaccatcgcc
    13501 agcagtaacg ctaagtcggc actggtttcg gttaccttca cgccgccgac cacgttcaca
    13561 aacacatcct gatcggccat ttgcagacca ccgtggcggt gcaacacagc cagcaggatt
    13621 gccagacggt tttgttccag ccccactgcc acgcggcgtg ggttcgccat catcgagtga
    13681 tcgaccagcg cctgaatctc caccagcagt ggacgcgttc cttcccatac caccatcact
    13741 gagctaccgg aggtcacttc atcgccgcga cttaagaaaa ttgccgaagg gttgctgact
    13801 tcacgcagcc cctgttcggt catcgcgaag acgcccagct cattcaccgc gccgaagcgg
    13861 tttttatggc tgcgtaaggt gcgaaaacgg gagtcggcat cgccatccaa aagcaccgaa
    13921 cagtcgatac agtgttccag cactttaggg cccgccagcg agccatcttt ggttacgtgt
    13981 ccaaccatga caatcgccac accgcgcatt ttggcgaagc gcgtcagata agccgctgtt
    14041 tcacgcacct gcgccacgct gcctggcgat gattgtacat ccgccatatg catcacctgg
    14101 atcgagtcaa ttaccatcag cttcggttgc tcttcttcgg caatcaggca gatctgttcg
    14161 atgctggttt ccgataacat attgagattg tcagtcggca ggccaaggcg atgagcgcgc
    14221 attgccacct gttgcagcga ctcttcgcca gtgacataca gcgttttcat ctgctgcgcc
    14281 agtttgcaca gcgtttgcaa tagcaatgtg gatttcccgg cacctgggtt accgccaatc
    14341 agaatggcgc ttcccggcac caccccgccg cctagtacgc ggtcgaactc tttaaatccg
    14401 gtggaaaaac gcggcagctc ttcaaggctg atatcggaga gtttctggac ttttgccacc
    14461 ccggcgctac cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg cgacgcagca
    14521 agacgcacct cggtgatggt gttccaggca tgacaggcac tgcactgccc ctgccagcgc
    14581 ggataatcgg ccccgcattc attacaaaca aaggcgcgtt ttggagcttt tgccacggtg
    14641 tacctcgtta atgctgtgcc gcccgcagga tgacgggcga gcaattactt ctgattcagg
    14701 ctgcctgaga ggatgcagaa tacccccatc aggtcagcgt gacggatggt gacttccgtc
    14761 ttttcattca cttttggctt ggcatggtag gcaatcccca gccctgctgc tttgatcatc
    14821 ggcaggtcat tggctccatc gccaatcgcc acggtctgcg ccagcggaat ttcatactct
    14881 tgcgcgaggc gggtcagagt tttcgctttg tactgcgcgt caacgatgtc gccgatcaca
    14941 ttgccggtaa atttaccgtc catgatctcc agttcattgg ctaccacggc ggtcaggcgc
    15001 agcttgtcgc gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat cgccactttc
    15061 cagcccagcg tttccagctt gagcaccagt tgcgttaagc ctggcatcag cggcagattt
    15121 tcacgcacct gttgcagaat attggcgtca gcgcctttca gcgtcgccac acggctgcgc
    15181 aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt cggttacttc cgccaccatc
    15241 tcgcccgttc cggccagttt ggcaatttca tcaatacatt caatctggat ggcggtggaa
    15301 tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc cagcggggcg
    15361 acatccagtt gcgcttcatg ggccaggcgc gtagcccgtg cggtgagtga acctgccaga
    15421 cgaatcacct gataatcttc cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt
    15481 ttgctctggt attgggtcag acgctgttta tccagcccac gaccatacag cagccagccg
    15541 ctacgacctg cgtggtaatc cagtggcatc acttcatcac cacttaatga aagaggcaga
    15601 cccggccata aagagacatc ttcaggcagg tcgcaccagg taatgttagg cattaaggct
    15661 cctgtaaaat cgttcgaagc agggaaaata acgcatgagg ctaccttgta tccattgctt
    15721 ctggcaacat taagtctcaa attttcaaag ggtggaagat ggctcgcaca aaactgaaat
    15781 tccggctgca tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg gcactgatgc
    15841 agggggcgtc atggtttagt caaaaccacc agcgacagcg taatccacag ctggaagaac
    15901 tggcccgcac cctggcgcgt caggtgacgc tgaacgttgc accgctgatg cgtaccgact
    15961 caccagatga aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa agccgcatcc
    16021 tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc ccgttcaggc gaaagcgtcg
    16081 aagtgcgcga ccggctggcg ctcgacggta aaaaagcagg cggctatttt aaccagcaga
    16141 ttgtcgagcc aattgcgggt aaaaacggac cgctcggcta tctgcgcctg acactcgaca
    16201 cccatacgct cgccaccgaa gcccaacagg tggataacac cactaacatt ttacgcctga
    16261 tgttgctgct ctcactggca atcggcgtag tgctgacccg cacgctgcta cagggtaaac
    16321 gcacccgctg gcagcaatcg cccttcctgt taaccgccag caaaccggtg ccggaagagg
    16381 aagaaagcga gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt atgtccacat
    16441 tacgcctgct catctctgac tcttacgacc cgtggtttaa cctggcggtg gaagagtgta
    16501 tttttcgcca aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg
    16561 tagtgattgg tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg atggaagaag
    16621 ataacgtccg cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac gatctcggca
    16681 atacctgctt tacctttatg gctggcaagc cggagtacga taaaaccatc tctacgtcga
    16741 ttgtgctcaa tgcgctgaac gcgctcggcg tcagcgccga agcgtccgga cgtaacgatc
    16801 tggtggtgaa aacagtcgaa ggcgaccgca aagtctcagg ctcggcctat cgcgaaacca
    16861 aagatcgtgg cttccaccac ggcactttgc tgctcaatgc cgaccttagc cgcctggcaa
    16921 actatctcaa tccggataaa aagaaactgg cggcgaaagg cattacctca gtgcgttccc
    16981 gcgtgaccaa cctcaccgag ctgctgccgg ggatcaccca tgagcaggtt tgcgaggcca
    17041 taaccaaggc ctttttcgcc cattatggcg agcgtgtaga agcggaaatc atctccccgg
    17101 acaaaacgcc agacttgcca aacttcgccg aaacctttgc ccgtcagagt agctgggaat
    17161 ggaacttcgg tcaggctccg gcattctcgc atctgctgga tgaacgcttt agctggggcg
    17221 gcgtggaact gcatttcgac gttgaaaaag gccatatcac ccgcgcccag gtgtttaccg
    17281 acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc tgcctgtacc
    17341 gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg gaccaggaaa
    17401 aagagctacg ggagttgtcg acgtggatag cgggggcggt aaggtaactc gctgattaaa
    17461 ataccaaagg aagggaatat gatcgatctg gaaaaccagg agcgggagat tatcaattta
    17521 atgttaagcc agcgtatttc ctggctagcc gccgtgcgta tcaggcataa attatcgctt
    17581 gctgaagtaa gtaaaatgtt aggtatctcg attaattctc taaaatagat cgaaaagacg
    17641 gaacggttta gctcaaacat caaaagcaaa atggcagaaa tttatggttg tccaccagag
    17701 ttgctgatat gcccttcctg gatgacggct gaacataaat gaatagattc gtgaatagat
    17761 acttgaatag attttatttt ctatccatat gaaaaataat agtaaatcta taaatcagtt
    17821 gctattgatg aatagatact aaaaagccgg agcagtctcc cactccggct tcacacgaca
    17881 atcgcctttc agcgaaacac aattactctt tatcgcccag cagaacggat tccagtgcga
    17941 ttttgatcat gtcgttgaag gtggtctgac gctcagcggc agtggtctgc tcgtgagtgc
    18001 ggatgtggtc agacacggta cagatggtca gggctttcgc gccaaattct gcagcgacgc
    18061 cgtagatacc agccgcttcc atttccacgc cgaggatgcc gtatttttcc atcacgtcga
    18121 acatttcgcc gtccggagag tagaacaggt cagcggagaa caggttacca acgcgagcat
    18181 caatgcccag tgctttagcc gcgtctaccg cgttacgcac catgtcaaag tcagcgatag
    18241 cggcaaagtc atgatcttta aaacggatgc ggttaacttt ggaatcggtg caggcaccca
    18301 taccgataac gacgtcgcgc agttttacgt gcggcagaac tgcgccacag gaacccacgc
    18361 ggataatttt cttcacgccg aaatcggtga tcagttcttt ggtgtagatg gagcaggacg
    18421 ggatacccat accgtgaccc attacggaaa ttttgcggcc tttgtaagta ccggtgaagc
    18481 ccagcatacc gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt tcagcaatat
    18541 acttcgcacg cagcgggtcg cctggcatca aaactacgtc agcgaaatcg cccatttctg
    18601 cattaatgtg tggggtagcc attgttttat ccttatcaac atgttttcac cctctccctg
    18661 agggagaggg ccggggtgag ggcatcaagc cgcacccatt atcagaacat ggctttgcca
    18721 tattccatat cagaagtacc aaaatatttt gccagagtct ggccgatatc cgcgaaggtt
    18781 tcacgatgac ccagtgagcc cggttttact ttcgggccgt ataccagtac cggaatgtgt
    18841 tcacgcgtgt ggtcagtacc ggtccaggtc ggatcgcaac cgtggtcagc ggtgaggatc
    18901 aggatgtcgt catcgcgcag cagagacatc agctccggca gacggcggtc gaacagttcc
    18961 agacccgcgg cataaccggc gacgtcgcga cggtggcccc aggaagagtc gaagtcaacg
    19021 aagttggtga agacgatggt gttatcaccc gcttctttca tctctttgat ggtggcgtca
    19081 aacagcgcgt ccaggccagt cgctttcact tttttggtga taccgcagtt ggcgtagatg
    19141 tctgcaattt taccgacaga aaccacctgg ccgtgttttt catcaaccag tttctgcagc
    19201 acggtcggtg ctggcggctc aacagccagg tcgtgacggt taccggtacg ctggaagtta
    19261 ccggctttgt caccgataaa cggacgagcg ataacacgtc cgatgttgta gccgccgttg
    19321 gtcagctctt cacgggcgat ttcgcacagt tcgtagagtt tatccaggcc aaaggtttct
    19381 tcatggcagg caatctggaa cacggagtca gcggaggtat agaaaatcgg cttgccggtt
    19441 ttcatgtgct cttcgcccag ttgatccaga atgaccgtac cggaagagtg gcagttaccg
    19501 aggtaacccg gcagattagc gcgttcgacc agtttatcca gcagctcttg cgggaagctg
    19561 ttttcgtgat cggagaaata tccccactca aacagaaccg ggacaccggc gatttcccag
    19621 tgaccagacg gggtatcttt accggatgac atttcgtgcg cccatgcgta cgcgccgata
    19681 acttcagcgt tgccgtccat tcccgccgga atgaaaccgg tagaaccttc gtgtgctttc
    19741 gccagcccca gacgggtcag atttggcaga ttgagcgggc ctttacgacc gttatcagct
    19801 tcgcctttgg cacaagcttc tgcgatatga cccagggtgt cagccccgac gtcaccaaag
    19861 cgttctgcat cttctgtagc gccgatgccg aatgagtcca gcaccataat aaatgcacgt
    19921 ttcatatgtt ctccgtactc agtgcttcaa atttaaatga tcagatcagt ataccgttat
    19981 tcgctgatac ggcgatagac agttggtgtg ctttccggtg ctttatcggc aagtttaatt
    20041 gccgctttca ccgctttcgc cgcttcctgc cagctgtttt cgtctttcgc gtggataacc
    20101 gccagcggac gctgaccgtc tacctggtcg cccagacgtg ccatatcagt aaagccgacg
    20161 ctgtaatcga tggtgtcaga tgcctgacgg cgtccgccgc ccattgcaac cactgccatc
    20221 cccagcgcgc gggtatccat ttcactgaca aaaccttcgg tatcagcata gactgctttc
    20281 gtcagcatcg ctgtcggcag atacttcgcg tagttctcaa cgaagtcggt tgggcctttt
    20341 tgtgccgcta ccatacgacc aaagacttct gccgctttac cgttgtccag caccgcctgc
    20401 aatttcgcgc gcgcttcggc gtcatctttc gccagtttgc cggagatcag catctccacg
    20461 cacagcgcca tcgtgacatc aaacagacgc gggttacgat attcacccgt caggaactgc
    20521 accgcttcac gaacttcaac cgcgttacct gcactggagg ccagtacctg attcatatcg
    20581 gtgagcagcg cggtggtgcg cacgccagcg ccgttagcca cgccaacaat cgcttcggca
    20641 agggcttcag agagttcgta ggtcggcata aacgcgccgc tacccacttt cacgtccatc
    20701 accagcgcgt ccagaccttc cgccagtttc ttcgccagga tcgaggcagt gatcagcggg
    20761 atggagtcca cggttgcggt aatatcacgg gtcgcgtaga aacgtttatc cgccggagcc
    20821 agtgagctgg tctgaccgat aatcgccacg ccgacgtctt taataatttc gcggaaacgg
    20881 ttgtcatccg ggaaaatgtc gaagccaggg atggattcca gtttgtcgag cgtaccgcca
    20941 gtatgaccga ggccgcgacc ggagatcatc ggaatatagc cgccgcaggc tgcgaccatc
    21001 ggccccaaca tcagcgaagt cacatcgccg acgccgccgg tggagtgttt atcaacaatc
    21061 gggccattca gatgcaggct tttccagtcg agaacggttc ctgaatctcg catcgccatg
    21121 gtcagcgaga cacgctcagg cattgtcata tcgtggaaga aaatggtcat cgcgagggcg
    21181 gcaatctgcc cttcggagat agtgttgtcg cgaatgccgt tgataaagaa acgaatttct
    21241 tcatcgctca gcgcatgacc atcacgtttt ttacgaataa tttcttgtgc gagaaacaag
    21301 gtaacctccc gaggaaaaga gtatgaggcg gcttttgccg gatgcgtcta cgccttatcc
    21361 ggcctacggg tagatacgtc tcgtaggcct gataagcgca gcgcatcagg cgtaaagcat
    21421 cttacttagt agctgctggc gctcttaccg tcaccgtggc ccagcgcttt cagcaggctt
    21481 gccagcaggc tggaagcacc aaagcggtag tgacgcgcat cagcccagtc agcaccgaaa
    21541 agttcatctg cgatggcgag atatttctgc gcatcttccg cagtacgcac gccgcccgcc
    21601 ggtttgaaac caacggtttt ttctacgccc atatcacgga tcacttccat catgatgcgc
    21661 gcgctttccg gcgtcgcgtt cacagccact ttaccggtag aggttttgat gaagtccgca
    21721 cccgctttga tggagatttc agacgcttta cggatcagcg cttcgtcttt cagttcgccg
    21781 gtttcgatga tcactttcag cagtacattc gctgccgcgc aagcctcttt acaggctttc
    21841 acaaggtcaa aaccaacctg ctcgttgccc gccatcagcg cgcggtacgg gaacaccacg
    21901 tcaacttcat cggcaccgta ggcgattgcc gcacgggttt ctgccagcgc gatttcgatg
    21961 tcgtcgttac catgtgggaa gttggttacc gtagcgatac ggatttccgg ggtgccctgc
    22021 tctttcagtg ttttgcgagc aatcgggata aagcgaggat agatacagat agcggcggta
    22081 ttgccgaccg gggttttggc ctgatgacac agagcaatta ctttctcgtc ggtgtcgtcg
    22141 tcattcaggg tggtcaggtc catcaatttc agtgcacgca ggctgcttgc tttcagatca
    22201 gtcatttcat tctcctggct tgtcgccaat aaaattcacc ttgcgagttt gttagtattc
    22261 taacatctac tccgcaacac acttcgatat acatcacaat taaggaaatc tacttacaag
    22321 tttgcatcac tgtaatgcga tctggttcaa ataattcact ttcaaatgaa tgcgtcagtg
    22381 gtagcaaacg catcaggatc aaagtgaaca tcacgaaact tcttacaatg gcgcatcacc
    22441 ttcggcataa aaggaaaaga agatgcccac ttctcatgaa aatgcactgc aacaacgttg
    22501 ccagcaaatt gtcaccagcc cagtgcttag cccggagcag aagcgccatt ttctggcact
    22561 tgaagcagaa aacaatctgc cttatccgca gctgcctgcc gaagcccgcc gtgcgctgga
    22621 tgagggtgta atctgcgata tgtttgaagg tcatgcgccg tacaaaccgc gctatgtctt
    22681 acccgattac gcccgttttc tggcgaacgg ttccgaatgg ctggagctgg aaggcgcgaa
    22741 agatcttgat gacgcactct ctctgctgac cattctttac caccacgtac cgtcggtcac
    22801 atcgatgccg gtctacctgg ggcaactgga tgcgttgttg caaccgtatg ttagaattct
    22861 aacacaagac gagatcgata ttcgaataaa acgtttctgg cgttacctcg acagaaccct
    22921 gccagacgcc tttatgcacg ccaatatcgg cccgtctgat tcgcccatta cccgtgcaat
    22981 cttacgtgca gacgcagagt tgaagcaggt ttcaccgaac ctgaccttta tctacgatcc
    23041 tgaaatcacc cctgatgacc tactgctgga agtggcgaag aacatctgtg aatgtagcaa
    23101 accgcacatc gccaacggtc cggtgcatga taaaattttc acaaaagggg gctacgggat
    23161 tgtgagctgt tacaactcac tgccgctggc gggtggtggc agcacgctgg tacgccttaa
    23221 cctgaaagcc attgccgagc gcagcgaatc gctggatgac ttctttacgc gcactctacc
    23281 gcactactgc cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca
    23341 atcacacttc tttgagaata gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt
    23401 tgtgccaatg tttggcatgt atgggctggc ggaagcggtt aacttgctgt gtgagaaaga
    23461 agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag
    23521 cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc
    23581 catgttacac gcacagtcgg ggatcagttc cgatatcggc accacgccgg gcgcgcgttt
    23641 accctatggc gatgagccag atccgatcac ccatctgcaa actgtcgccc cgcatcatgc
    23701 ttattattat tccggcatca gcgacattct gacgctcgac gaaaccatca aacgtaatcc
    23761 gcaggcgctg gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc
    23821 caatgtcagc ggtaacgatc tggttcgcgt taccggttat atggtgcgtt tgtcggattt
    23881 agaaaaatat cgcgccgaag gttcacgcac caacaccacc tggctgggcg aagaagccgc
    23941 acgcaacact cgtattctgg aacgccagcc gcgcgtgata agccatgaac agcagatgcg
    24001 ctttagtcag taagattatc cccttctcct gcgttgacgg gccaggcagt cgtctggctc
    24061 tgtttttgca gggctgcaat ctgcgctgca aaaactgtca caatccgtgg acgatggggc
    24121 gctgtaatca ttgcggagag tgcgtgccac agtgtccgca tcaggcgttg cagattgttg
    24181 acggcaaagt ggtgtggaac gctgcggtct gcgagcagtg tgatacctgc ctgaagaggt
    24241 gtccgcaaca tgccacgccg atggcgcaat ccatgagcgt ggacgaagtg cttaaccatg
    24301 tccgcaaagc ggtgctgttt attgaaggga taacggtaag cggcggcgaa gccacgaccc
    24361 aactgccgtt tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc
    24421 tcacctgtct ggtggacagt aacggcatgt tgagcgaaac cggctgggaa aaattactcc
    24481 cggtgtgtga cggcgcaatg ctcgatctca aagcatgggg tagcgaatgt catcaacaat
    24541 taaccggacg cgataatcag cagattaagc gcagcatctg tttgctggca gagcgcggca
    24601 agctggcgga actgcgtttg ctggtaatcc ctggccaggt ggattatttg caacacatcg
    24661 aagaactggc ggcgtttatc aagggacttg gcgatgttcc ggtacgcctg aacgcgtttc
    24721 atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg
    24781 agccgttggc tgatgcatta aaggtgcgcg gggtgggccg gttgatattt ccggcgctgt
    24841 atttgtgaca tgccggatgc ggcctacggt tcggcacaga cttgtaggca tgataagacg
    24901 cgtccagcgt cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc
    24961 cggcctacgg ttcggcacaa acctgtaggc ctgataagac gcgacaagcg tcgcatcagg
    25021 cattatgcgc cgactgccgg atgcggcgtg aacgccttat ccggcctacg gttcggcaca
    25081 gacttgtagg catgataaga cgcgacaagc gtcgcatcag gcattgtgcg ccaactgccg
    25141 gatgcggcgt aaacgcctta tccggcctac ggttcggcac agatttgtag gcatgataag
    25201 acgcgtcaag cgtcgcatca ggcattgtgt gccgactgcc ggatgcggcg tgaacgcctt
    25261 atccggccta cggttcggca caaacctgta ggcatgataa gacgcgacaa gcgtcgcatc
    25321 aggcattatg cgccgactgc cggatgctgc ctacgaaggg taaactaccc actaacgcta
    25381 aacaacgcat acgtattatt aagcaacact tcggcaatct catcttctgg ttccgggcgc
    25441 aactcgcaaa gcacgtcaaa cacccgcaca gcctgttccg gtcggttagg ctgcccctga
    25501 aagccgttga gcggcatatc cggcgcgtcg gtttccagca acaacgatgc cagtggtaat
    25561 tttgcgatga catcgcgggt tttactggcg cgtggatagg tgatagtacc gcctacgccg
    25621 attttgtagc ccagctgtac aaaccgttca gcctgttgct ggctgccaga aaatccgtgg
    25681 acaacaccag cacgcggcaa atcgtggcgt ttaagatgca tcgccagttt gtcgtgtgtg
    25741 cgccgtgaat gcaggatcac cggcagatcg tagcgtttcg ccagcttcag ttgttcgtcg
    25801 agtaaccact gctgcctctc aaattgcgga tcgtcgccga agagatccag accgatctcc
    25861 cccaccgcca ccaccttcgc cggacgcctt tccagcgcct gctgtagctg atcaagagac
    25921 acatcgctat gtttttccaa catccccgga tgcaacccca atgcggcata cagcggttga
    25981 tatttttccg ctaacgcctg cacgcgggca aaattttccg cctctgtcgc cggaacaatg
    26041 atcttgccta cgcccgcttg tgccgcgcgt tgcaggctgg cctcttcatc gccactaaac
    26101 ggcgggaaat caaaatggca gtgggtatcg ataaaacggc aaatcaagcc agatcctcat
    26161 tgttaaatgt cgggtcgttc gcctgcggag catcgctcac ttcggcaacc agcgtatcgt
    26221 tggcgactgg cgcaggcgga ataacgatcg attccggcgt caccactggc accagatggc
    26281 gggtaagcgg cgctttttca gttaatagct tgccaaccgt ggcgaggaaa taacgaccgc
    26341 ataaacgccc aagtttatag tcttcacgca gcgccggaac ccgactgcca agcgcgatac
    26401 tatgtaatgg cttcggcgga taaatttcga atatccgcag cttgcccggt ggtttctcaa
    26461 taaattgctg aatgtcacga tagctggttt catgatgctg caccagattg accagcggct
    26521 gcaggctact gtcacccagc cagcgttcca tgcgtttgaa ccactgcggc gtgtagtaca
    26581 tttgtgacgg cacagtgcgg atgacgacca acgttttagc gccctgcctt gccgcctctt
    26641 taaccggaat cgcatcactg atcccgccat ccaggtagtt aatgccttcc agcgacactc
    26701 cgctacgata aaagccaggt atcgcactgg aggcgcgaat cacatccagc cagttttgtt
    26761 tggttggtaa aaagtaattc ggcgcgtagt catcctgacg acaggcgcac atataaaacg
    26821 atttgccgct gtcaaacaac cgcgcggcgg tatccatttg caacggcatc tggcttgcgg
    26881 tggcctccac cagccagtcg agatcgataa gatttcctcc acggacaaag cgcaatggat
    26941 cgaaaaattc gcgttttgtg gtatagcgca tgatgacttt gcgcgcgtaa ccgggctgat
    27001 tgcagataaa cgccgagagg ttctgcgccc cggcagatgt gccgagataa agatcgaaag
    27061 gattaaactg cgcgcgcata aactcatcca gcacgccagc cgtgaaaatt ccacgctgtc
    27121 cgccgccttc gcacaccaga gctattcgtc caggctggaa cggccttagc gacaacggcg
    27181 caatattacc aagcgttaca ggtattcgct gccccacctc tgccttcctg tttttgttgt
    27241 gaaagaatga caaagtaacg caatttatta ttcgctaaaa ccgcaaaagc cagtccgctg
    27301 gactggcttt ggataaaatt ttcttaatgg tctgaaatct agggtcgttt tcggcccatg
    27361 aacaaactca ccaggaacag aataatcccg acgacaaaga caattttagc tgcgcctgca
    27421 gcggtaccgg ccagaccacc aaacccaagt gcggcggcga ttaacgcgat aaccagaaat
    27481 atgatgcccc aacgaaacat aagcgtctcc tttaccatag ttaatgtcac cgcttgatat
    27541 gagcgtgaaa atcactcaat gcgatatttt tagtgtggtg cacatcgcgc ctcccgacaa
    27601 agttcgggag gacgaattac gacgaattac ttagttttca gatcattttt aacgcttttc
    27661 acaccatcta ccgctttggc gatactttca gcacggtcac tttgtgcctg agaatctacg
    27721 gtaccggaga gctgaaccac gccatcggtg gtttcaactt tcacatgacg ggaagggacg
    27781 atatcgtccg ccagcagttt ggctttgatt tcactggtgg tggcggtgtc acccgcgtag
    27841 cccttcaccg agccttcttt agcgtcgcga acgtgcagtt tgtcgctgac agaggtcacc
    27901 ccttcaacgc ctttcgccac tttcactgcc tcttcggcct gagcctggct ttcaacgaaa
    27961 ccgctcaggg tcacgacttt ttgatcggtt tttacagaga tatcggtgct cttgatgttg
    28021 tcatgatcca ccagggccgc cttcactttc gcggtgatgg cgctgtcatc catgaaatta
    28081 ccgactttat tcatagagct atcgactttt tgccctgcgc tttcattggt agtctgtgcg
    28141 ttgttttccg cgtaggcaga gccggtcgcg acggcagagg tcaacattac agccagcaga
    28201 gttttcgaaa tcttcagtct tgtcatagtc atcgatttat tcctgtatgt ttgctcgtaa
    28261 tttgagcctg gcaacacgag gttgcattgc tgaataggga gagacttcac cctctacaga
    28321 agtcaatggt cgccatcaca aaagcgatgg gtgatgaata acgaccatta cagcctctga
    28381 atcagttatt aatatcggta gaatgacaat cgacggcttt agatactgat atctacgcat
    28441 tgaacggtat ttaacgccgt cagaaatgtc attactttgt taaatataga tcacaatttt
    28501 gaaaccgctc gggatatcag cgaaaacata agcaaaagtg aatgttttaa gaacattccg
    28561 taagcggcta ataaggaagg gaaattgaca gggcgcagcg gctaccgcgc cctggaggca
    28621 agaattaatg ctcgcgggtc tggtggaact gaacgtcggg ataacgttcc tgcgccaggc
    28681 gcaggttgac catgctggta gcgatgtaag cgaggttatc gccgccatca agcgccagtt
    28741 ggctttcgtt cttacgcttg aactcttcga atttcttcgc gtccgcacat tctacccagc
    28801 gggcagtggc aacgttgact gattcatata ctgcttcaac gttgtattcg ctcttcaggc
    28861 gcgataccac cacatcaaac tgcagcacac caacagcacc aacgatcaaa tcgttgttgg
    28921 agatcggacg gaacacctgc accgcgccct cttcggaaag ctgtaccagc cctttgagca
    28981 gctgtttttg cttcagcgga tctttcaggc ggatacgacg gaacagttct ggtgcgaagt
    29041 tcggaatacc ggtgaacttc atcatctcac cctgggtaaa ggtgtcgccg atctgaatag
    29101 tgccgtggtt gtgcaggccg aggatatcgc ccggatacgc ttcttcaacg tgcgaacggt
    29161 cgcccgccat aaaggtcagc gcgtcggaga taaccacatc tttcgcagta cgcacctggc
    29221 gcagcttcat gcctttttca tatttaccgg atactacgcg cataaacgcc acgcggtcgc
    29281 ggtgtttcgg gtccatgttg gcctgaattt taaatacgaa gccggtaaat ttgtcttcgc
    29341 tcgcctctac ggtacgggta tcagtctgac gcggcatcgg cgcaggggcc cactccacca
    29401 ggccatccag catatgatca acgccgaagt tacccagcgc agtaccgaag aataccggag
    29461 tgatttcgcc cgcaaggaac aattctttgt cgaactcgtt agacgcgcct ttcaccagtt
    29521 ccagttcgtc acgtagctgc tgtgccagat cttcaccaac cgcagcgtcg agatccgggt
    29581 tattcagccc tttaacaata cggacttcct gaatcgtgtg gcctttaccg ctctgataga
    29641 gataggtttc gtctttataa aggtggtaaa cgcctttaaa cagcttgccg cagccaatcg
    29701 gccaggtgat cggtgcgcag ccgattttca gctcgttctc aacttcatcg agcagttcca
    29761 tcgggtcgcg gatatcacgg tcaagtttgt tcataaaggt gaggatcggc gtgtcgcgca
    29821 gacgggtaac ttccatcagc ttacgggtac gatcttcaac accttttgcg gcgtcgataa
    29881 ccatcaggca gcagtccacc gccgtcaggg tacgataggt atcttccgag aagtcttcgt
    29941 gccccggggt gtcgagcagg ttaaccaggc aatcgtgata cggaaactgc atcacagacg
    30001 tagtaatgga gatcccacgc tgcttttcca tctccatcca gtccgactta gcgtgctggt
    30061 tggaaccacg gccttttact gtaccggcgg tctgaatggc ctgtccgaac agcagcacct
    30121 tctcggtgat ggtagtctta ccggcgtccg ggtgagaaat aatggcaaaa gtgcggcgct
    30181 tcgccacctc ttgcaaataa ggagacaacg tcataattct ttcttcttga gtaagcggca
    30241 tcgcgccgcg cttgttgaaa acgaaaaatt gcggctattt tacccatcaa cgggggggag
    30301 gcaatcagtg tttacacagg agctgctcca gttcgtgcaa cgatgaaacg gtccaggtgg
    30361 gcgcgatgcc ttctggttgc tcgcgatggt gcgcattcag ccagcaggtc gcaagcccgg
    30421 cgttgatgcc accgagaata tcggactcag cagtgtcgcc aaccatcagc acgcgtgaac
    30481 ggtcaggatt gcccgcctgt tccagcgcat aatcgaaaat tttcttattc ggtttagcaa
    30541 cgccaacttc ttcggaaatc accagcaaat cgaagtaatc acgcaggccc gtacgttcca
    30601 gacgcacttg ctgcaaggcg ctaaagccgt tggtgatgat gccgattttg gcgttgccac
    30661 gaatggcgtt aagcagagaa accgcgcccg gcagcggcgt acagatttcc gccatcgcat
    30721 taataaaggc ttcgttgagt ttacccggct cgacgttcag ccgttcggcc cagctctcaa
    30781 accgcccgtg ctgaagctgt aatgaagtga tcgcgccgtt ttgataatcc acccacagtg
    30841 gcttgttaac ggcctgatag tcctgaaaat cttcagcggt aaaggtgacg ctgtaatcaa
    30901 gaaacatccg ctgcaggccg gtgaatgagt caaaggtaaa cagcgtttca tcggcatcaa
    30961 agaaaatcca gtcccacttc attattccac cttgtattgc atactgattg gcaacgccat
    31021 gatgatggcg tcttcgcgac cgtccgtggt ggggtagtaa ttgcggcgaa tcgtcgcctc
    31081 gttaaagcct aaactttcgt acagggcaat ggcggcagcg tttgaggcac ggacttccag
    31141 ccatagcgtc gccacaccgc gtttttccag ttcgtcgatc agatgttcca gcagcgccct
    31201 tcccaatccc tgacgctgat agtcgggatc gaccgcaata ttgaacaatg tagcttcatc
    31261 cagcaccact tgcgtaatcg caaacgccgc cattttgccg ttttgcgtta actgaaagtt
    31321 gagataacgc tcgccctggt tgctggcaaa ggttttttca ctccacggaa aggcgtgggc
    31381 gcgttgttca atgtggtaag ccgccggtaa atcagtcgtt tcgagggaag aaatcgtgtt
    31441 catatgtgca aatctgttgc cataacgcgg cgcgtgccgt tgggtttgcc cgtaattcgg
    31501 tgagcgccgg tgatgccacc tgagcgcctt ccagtgatag cggttcgtca gtacccaacc
    31561 gccagctgtt gcagcgactg ccttgcggca gcatcgcgat tttttctggc gtcagttgca
    31621 gcacctggtc ggggctgacg gttaatgcgc gcagaacatc gctcactaaa ggatcagtca
    31681 gggcgggaag atcgtttgcc accatcacca gacggacgtg tgccgggatc gcaatggcaa
    31741 tctccccctg caacgcgcca gggcgacgca gcgaccactg ggtaatgccc agttgctgta
    31801 actgccagtc tcgtcgggat gtcatagcga atcgctcctg ttgtcagggg cgcaaatata
    31861 gcaaattcgt cgataccgcg ccaacatatg gctataatcg ccgccagtat caattgagga
    31921 gcattccatg tctgcattta ccccggcaag tgaagtcttg ctgcgtcaca gtgatgattt
    31981 cgaacaaagc cgtattctgt ttgccggaga cttacaggat gacctgcccg cgcgtttaga
    32041 taccgcggcc agccgtgctc atacccagca attccaccac tggcaggtgt taagccgcca
    32101 gatgggggat aacgcccgtt ttagtctggt cgccacggcg gatgacgtcg cagattgcga
    32161 tacgctgatt tactactggc cgaagaacaa accggaagcc cagttccagt tgatgaattt
    32221 actttctctg ctgccagtgg ggacagatat ttttgtcgtt ggcgagaacc gcagcggcgt
    32281 gcgcagcgcc gagcagatgc tggcagatta tgcgccgttg aataaagtcg acagcgctcg
    32341 tcgctgtggc ctctattttg gtcgtctgga aaaacagccg gtatttgatg ccgataaatt
    32401 ctggggcgaa tacagcgtcg atggcctgac ggtcaaaacg ctgcctggcg tgtttagccg
    32461 cgacggtctg gatgtcggta gccagttgct gctctcgacg ttaactccgc acacgaaagg
    32521 taaagtgctg gatgtcggct gtggcgcggg tgtgctttca gttgcctttg cgcgccattc
    32581 gccgaaaatc cgtctcacct tgtgcgatgt ctctgccccg gcggtagaag ccagccgcgc
    32641 gacgcttgcg gccaacggtg ttgaaggtga agtctttgcc agcaacgtct tttccgaggt
    32701 gaaaggtcgt tttgatatga tcatctccaa cccgccgttc cacgatggga tgcaaaccag
    32761 cctggatgcg gcgcaaacgc tgattcgcgg tgcggtgcgt catcttaata gcggcggcga
    32821 gctgcgaatt gtagcgaacg ccttcctgcc ttatccggac gtgctggatg agacatttgg
    32881 cttccatgaa gttatcgcgc aaaccgggcg cttcaaggtg tatcgcgcca ttatgacccg
    32941 ccaggcgaag aaaggttaag tatcccgccg caagccttgc cggatgcggc gtaaacgcct
    33001 tatccgacat accaaacctg cacgttagcc cttgtaggcc agataagacg cgccagcgtc
    33061 gcatctggca tctgcccccg aatgccgtgt cggatgcggc gtgaacgcct tatccgacct
    33121 acaaacgaat tatccacaat ccgctgatgg ccatttttcc agcaattaca cctccggcga
    33181 taattaacta ttgacgaaaa gctgaaaacc actagaatgc gcctccgtgg tagcaattct
    33241 ttttaagaat tgatgg
//
LOCUS       DAOFQD010000048        32558 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.048, whole genome shotgun sequence.
ACCESSION   DAOFQD010000048 DAOFQD010000000
VERSION     DAOFQD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.048"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(165..431)
                     /gene="ariR"
                     /locus_tag="QBF46_002255"
     CDS             complement(165..431)
                     /gene="ariR"
                     /locus_tag="QBF46_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HDQ6674431.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(460..732)
                     /locus_tag="QBF46_002256"
     CDS             complement(460..732)
                     /locus_tag="QBF46_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDQ6674432.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(775..1011)
                     /locus_tag="QBF46_002257"
     CDS             complement(775..1011)
                     /locus_tag="QBF46_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDQ6674433.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1325..2536
                     /gene="bluF"
                     /locus_tag="QBF46_002258"
     CDS             1325..2536
                     /gene="bluF"
                     /locus_tag="QBF46_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDQ6674434.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            2741..3472
                     /gene="bluR"
                     /locus_tag="QBF46_002259"
     CDS             2741..3472
                     /gene="bluR"
                     /locus_tag="QBF46_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDQ6674435.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3693..4097
                     /locus_tag="QBF46_002260"
     CDS             3693..4097
                     /locus_tag="QBF46_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDQ6674436.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            4150..4260
                     /locus_tag="QBF46_002261"
     CDS             4150..4260
                     /locus_tag="QBF46_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674437.1"
                     /translation="MEIKVQRLSLWMINTVFLLSPINNHQTNTINLIFEM"
     gene            4496..4540
                     /locus_tag="QBF46_002262"
     CDS             4496..4540
                     /locus_tag="QBF46_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674438.1"
                     /translation="MFSKLAQSSIKAMF"
     gene            4797..5120
                     /gene="iraM"
                     /locus_tag="QBF46_002263"
     CDS             4797..5120
                     /gene="iraM"
                     /locus_tag="QBF46_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDQ6674439.1"
                     /translation="MKWIVIDTVIQPTCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYHIATFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(5223..6473)
                     /gene="icd"
                     /locus_tag="QBF46_002264"
     CDS             complement(5223..6473)
                     /gene="icd"
                     /locus_tag="QBF46_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDQ6674440.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            6645..7298
                     /gene="rluE"
                     /locus_tag="QBF46_002265"
     CDS             6645..7298
                     /gene="rluE"
                     /locus_tag="QBF46_002265"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDQ6674441.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7308..7769
                     /gene="nudJ"
                     /locus_tag="QBF46_002266"
     CDS             7308..7769
                     /gene="nudJ"
                     /locus_tag="QBF46_002266"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDQ6674442.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            7823..8929
                     /gene="mnmA"
                     /locus_tag="QBF46_002267"
     CDS             7823..8929
                     /gene="mnmA"
                     /locus_tag="QBF46_002267"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDQ6674443.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            8965..9606
                     /gene="hflD"
                     /locus_tag="QBF46_002268"
     CDS             8965..9606
                     /gene="hflD"
                     /locus_tag="QBF46_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDQ6674444.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            9610..10980
                     /gene="purB"
                     /locus_tag="QBF46_002269"
     CDS             9610..10980
                     /gene="purB"
                     /locus_tag="QBF46_002269"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDQ6674445.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHVVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDDLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            11149..11820
                     /gene="phoP"
                     /locus_tag="QBF46_002270"
     CDS             11149..11820
                     /gene="phoP"
                     /locus_tag="QBF46_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDQ6674446.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHI
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            11820..13280
                     /gene="phoQ"
                     /locus_tag="QBF46_002271"
     CDS             11820..13280
                     /gene="phoQ"
                     /locus_tag="QBF46_002271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDQ6674447.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNLLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            13356..14477
                     /gene="roxA"
                     /locus_tag="QBF46_002272"
                     /gene_synonym="ycfD"
     CDS             13356..14477
                     /gene="roxA"
                     /locus_tag="QBF46_002272"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDQ6674448.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14526..15752)
                     /gene="pepT"
                     /locus_tag="QBF46_002273"
     CDS             complement(14526..15752)
                     /gene="pepT"
                     /locus_tag="QBF46_002273"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDQ6674449.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16002..17138
                     /gene="potA"
                     /locus_tag="QBF46_002274"
     CDS             16002..17138
                     /gene="potA"
                     /locus_tag="QBF46_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDQ6674450.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17122..17979
                     /gene="potB"
                     /locus_tag="QBF46_002275"
     CDS             17122..17979
                     /gene="potB"
                     /locus_tag="QBF46_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDQ6674451.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            17976..18770
                     /gene="potC"
                     /locus_tag="QBF46_002276"
     CDS             17976..18770
                     /gene="potC"
                     /locus_tag="QBF46_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDQ6674452.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            18767..19813
                     /gene="potD"
                     /locus_tag="QBF46_002277"
     CDS             18767..19813
                     /gene="potD"
                     /locus_tag="QBF46_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDQ6674453.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            19871..20332
                     /locus_tag="QBF46_002278"
     CDS             19871..20332
                     /locus_tag="QBF46_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDQ6674454.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            20329..21117
                     /locus_tag="QBF46_002279"
     CDS             20329..21117
                     /locus_tag="QBF46_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDQ6674455.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIALFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQST
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSRIFYYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(21237..22076)
                     /gene="cobB"
                     /locus_tag="QBF46_002280"
     CDS             complement(21237..22076)
                     /gene="cobB"
                     /locus_tag="QBF46_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415638.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDQ6674456.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGLKAG
                     SIA"
     gene            complement(22092..23003)
                     /gene="nagK"
                     /locus_tag="QBF46_002281"
     CDS             complement(22092..23003)
                     /gene="nagK"
                     /locus_tag="QBF46_002281"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDQ6674457.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPKIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVIIGGGLSNFPAITAQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(23032..24276)
                     /gene="lolE"
                     /locus_tag="QBF46_002282"
     CDS             complement(23032..24276)
                     /gene="lolE"
                     /locus_tag="QBF46_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDQ6674458.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(24276..24977)
                     /gene="lolD"
                     /locus_tag="QBF46_002283"
     CDS             complement(24276..24977)
                     /gene="lolD"
                     /locus_tag="QBF46_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDQ6674459.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24970..26169)
                     /gene="lolC"
                     /locus_tag="QBF46_002284"
     CDS             complement(24970..26169)
                     /gene="lolC"
                     /locus_tag="QBF46_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDQ6674460.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            26431..27504
                     /locus_tag="QBF46_002285"
     CDS             26431..27504
                     /locus_tag="QBF46_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDQ6674461.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALLLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            27632..31078
                     /gene="mfd"
                     /locus_tag="QBF46_002286"
     CDS             27632..31078
                     /gene="mfd"
                     /locus_tag="QBF46_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDQ6674462.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            31222..32184
                     /gene="ldtC"
                     /locus_tag="QBF46_002287"
     CDS             31222..32184
                     /gene="ldtC"
                     /locus_tag="QBF46_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDQ6674463.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(32267..32524)
                     /gene="bhsA"
                     /locus_tag="QBF46_002288"
     CDS             complement(32267..32524)
                     /gene="bhsA"
                     /locus_tag="QBF46_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDQ6674464.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         8274 a         8119 c         7906 g         8259 t
ORIGIN      
        1 ttttaaacaa agcacattga aaaataaatc tctctgggat tgaattattc atgtcagcct
       61 cgtagaaata ttaacccaat aaaattatgc tgtgtatagt ctaaagcgac agacaaaaat
      121 aagatgaatg agttttctat aaacttatac ttaataatta gaagttacat atcatcagct
      181 gtgtatcgca acacgatttc cagtgttttt ctaatcacat cagctttgag aatatcactt
      241 gttgtttcca gggagtgtat cagacttaag ataatatttt tattattaac attatcgcct
      301 gaaagcatta aattggtgac agcctgccct aacactgctg actcttcttc taacaaatta
      361 cccgaactgc gaaagtaact tgctaacgct ttatcagtta ttgcattatg aattgtagta
      421 tcttcaagca tggtaacctc tcatcttact tatgaaattt taatgtattc tgtttatttt
      481 cttaccattg aacatacttt tattgtcagc tgtcgctgtt cttcggtttg gccctcctga
      541 atcactattg ctaaaatcac gaattatgct gtcaattaaa tcgcgtcgga aagcatcttc
      601 ctcagccgat ttcattacga gtaattgctt aataagagtc tgcaatgaaa aatttatctt
      661 catgcgcagt atctggagca ccgcctggcc agtaatttca tattttatac gttcattatc
      721 agatgtcttc ataacgactt catagctaac ctgttaaata atcaggctgt tgcattattc
      781 aaaaagcaac ccaattagtg cattataatg tttctgttcc tcttccccag tcgcgtgttc
      841 cagtcggcaa agaagtttgg cacaaagcga ctttcgactc agattacgtc catctttcaa
      901 aatctcagtc acaatttccc cgagagtttc ctgctgagta ggcaagttag cttttgcaaa
      961 gtaacgagtg attgctcccg cagaatctaa agtcactgaa ttttgatgca tgctacgcct
     1021 ctgttaaaaa tgttaatcaa tgttaagtga ggtaacaata gcaaacttgt acgaaatatg
     1081 tgtacagaat gataaattaa ttttttgcaa ttagtgctaa tgcatataaa acaatgtgtt
     1141 atgataaaac atattgcatc atttaactga caatctatac aagatttttc gccaactagt
     1201 gttcaacttt ttccattttt tatcaatgta aagaaacggt tatgttttgg tattaaaaga
     1261 aagacacaag aagatatgtc tgttaccgtc ttactctcgc ctcacccatt accctggatt
     1321 gttaatgctt accaccctta tttatcgtag ccatatacgt gacgacgaac ctgtcaaaaa
     1381 aatcgaagaa atggtttcga tagcaaatcg caggaacatg cagtctgacg taacagggat
     1441 cttactgttt aatggttctc attttttcca gcttctggaa ggtccggaag aacaggttaa
     1501 aatgatatat cgggctatat gccaggatcc acggcactat aatattgttg agctgctgtg
     1561 cgattacgcg cctgctcgcc gttttggcaa agcgggaatg gaattatttg atttgcgcct
     1621 gcacgagcga gatgacgttt tacaggccgt attcgacaaa ggcacatcaa aatttcagct
     1681 aacttatgat gacagagcgc tacaattttt tcgtactttt gtccttgcaa ccgaacaatc
     1741 aacctatttc gagatccctg ccgaagactc ctggcttttt atcgctgacg gatctgataa
     1801 agaacttgat tcctgtgccc tttcaccaac tataaacgac cactttgcct ttcatcctat
     1861 tgtcgatccc ttatcgcggc ggataatcgc ttttgaagcc attgtgcaaa aaaatgaaga
     1921 tagcccatca gccatagcgg ttgggcagcg taaagacggg gaaatctaca cagcggatct
     1981 caaaagtaag gcgcttgcat tcacgatggc acacgcactt gagctcggtg ataaaatgat
     2041 ttcaatcaat ctattaccta tgaccctggt taacgaacct gacgcagtct cttttttact
     2101 taatgaaata aaggccaatg ctctggtgcc tgaacaaatc atcgttgaat ttactgaaag
     2161 tgaagtcata tctcggtttg atgagtttgc cgaagcgatt aaatcgctaa aggctgctgg
     2221 tatcagtgta gcaattgatc attttggcgc aggttttgct ggtttgttac tcctgtcacg
     2281 cttccagcct gacagaatta aaatcagtca ggaattgatt accaatgttc ataaaagtgg
     2341 gccacggcag gcaattattc aggcgatcat aaaatgctgt acatcacttg aaattcaagt
     2401 cagtgctatg ggcgtggcaa caccagaaga gtggatgtgg cttgaatccg caggaattga
     2461 gatgtttcag ggagatctgt ttgctaaagc taaattgaat ggtatccctt ccatagcgtg
     2521 gccagagaaa aaataatttt cagcacattc tttcacatga tttcagtaaa tcatcgtgaa
     2581 aatgaatacg ttacgtttaa atttaatcca cattaaaaat aatgtttcat ccaatttagc
     2641 tgaaatctta tttatacttt gtacaatttt agcgtatatt gtacaaagta ttattgggtc
     2701 gtgtacaggc gacggagatt tgtgaccgca aggaggaatt gtggcttatt acagcattgg
     2761 tgatgttgct gaacgttgcg ggattaatcc tgtcactctc cgggcctggc aacgccgcta
     2821 cggtttgtta aaaccacagc gcagtgaagg cggacaccga ctctttgatg aagaagacat
     2881 acaacgcatc gaagagatca agcgttggat aagtaatggc gtccctgtag gcaaagttaa
     2941 agcattactg gaaaccacca gccaggatac ggaagatgac tggagccgcc tgcaagaaga
     3001 gatgatgtca attcttcgca tggctaatcc tgccaaacta cgcgcgagaa ttatttcact
     3061 gggtcgagag tacccagtag atcaattgat taatcatgtt taccttcctg ttcgccagcg
     3121 tctcgtgctt gatcacaaca cctcccgcat tatgagcagt atgtttgacg gcgcattaat
     3181 tgaatacgca gcaacctcgc ttttcgaaat gcgccgtaag cccggtaaag aagccattct
     3241 aatggcgtgg aatgttgaag agagagcacg attgtggctg gaagcatggc gtttatcatt
     3301 gtcaggatgg cacatttctg ttcttgctga tcccattgaa tcgccgcgcc cggaactgtt
     3361 cccgacgcaa acattgattg tctggacagg catggcacca acgagaaggc aaaacgaact
     3421 tttacaacac tggggtgagc aaggttataa agtcatcttt catgccccct aatttcacct
     3481 ttatcccgcc ccttagacag aatgttataa tctgaaccca caacagcgtt tgtaccggag
     3541 ctggtccgtt gcaaaatgct caccgggaga attcagatat tccctctatc acactctgta
     3601 catatcacag ccaaaggaca gaccagattg aagtggtaaa accgatcgtg cacactacgc
     3661 agtctcacaa ctaacaagag gttattatcc tgatggatca ggtcgttgtt tttcaaaaga
     3721 tgtttgagca agtgagaaaa gagcaaaact tctcctggtt ttattcagaa ttaaaacatc
     3781 accgtattgc acattacatt tattatctgg ctacggataa catcagaatt attactcacg
     3841 atgatacggt tttgttatta agaggaacca ggaacctgtt aaaagttagt acgaccaaga
     3901 accctgctaa aataaaagag gccgcattgc ttcatatttg cggaaaatct acatttcggg
     3961 aatactgttc aacactggca ggcgcgggcg ttttccggtg ggttactgat gttaatcata
     4021 acaaacgcag ttactacgcc attgataata cgcttttata tattgaagat gtagaaaata
     4081 ataaaccatt aatctagcta aagttggata cttaagaaat gcttcataat tcagtaaggc
     4141 attagcataa tggaaataaa agtgcagaga ctatctctat ggatgattaa tactgtcttt
     4201 ttattgtcac ccataaataa tcaccagact aatactatca acttgatatt tgaaatgtga
     4261 tcacttgact tttgatacgt tattttataa cggttaacat atttataaaa acaacgaccg
     4321 tgccacacgt ccgtttcaat acttaacgca catgtatttt ggtttagtca tcatccggtt
     4381 atatgtattt tagccaggaa caggttaaat cattcctata taactcaaaa attgaaacct
     4441 tattctcatg tcatgcttat attcattatt atcgttatat aaaaaggcaa ccataatgtt
     4501 tagcaaattg gcacaaagta gcataaaggc tatgttttaa ttacaggatg ttcagtcatt
     4561 tgaatgtata acattatagc taaacaaatc taaaacgaag tcaataattt attgctttca
     4621 caaaatctca ttttgtttaa catccattga gattccttgc tttaaatttt attttatata
     4681 agccatcatt ttaattaatt tatctttttg aggggggggt aatatactca tatgcaaaat
     4741 caagaaataa acatcctaat gaaccatatt aaataccgtg ggataagaca taacaaatga
     4801 agtggatagt aattgacacg gtaattcaac ctacatgtgg tatatctttt tcagccatat
     4861 ggggtaatat gaaaatgatc atctggtatc aatctactat atttctccct cctggcagta
     4921 tatttacacc ggttaagtct ggtattatcc ttaaggataa agaatatcct attactattt
     4981 atcacatcgc gacattcaac aaggatttat ggagtttact caaaagcagt caagagtgtc
     5041 ctccaggaga aagcaaaata acaaataaat gtttacataa tagttgcatt ataaaaatat
     5101 gcccatatgg gctcaagtaa tgggcttcat ataaatatgc tcgatatgct attaataata
     5161 tccacataaa taaaacaacg ggcgtgttat acgcccgttt caatatttaa cacatggaga
     5221 gattacatgt tttcgatgat cgcttcacca aactctgaac atttcagcag cttagcgcct
     5281 tccatcagac gttcgaagtc ataagttacg gtcttggcat tgattgcgcc ttccatacct
     5341 ttaacaatca ggtcagccgc ttcagtccaa cccatatggc gcagcatcat ctcagcggag
     5401 aggatgatag agcctggatt tactttgtcc tgaccggcat atttcggcgc agtaccgtgg
     5461 gtggcttcaa acagggcgca ttcgtcaccg atgtttgcac caggggcgat accgataccg
     5521 ccaacctgcg ctgccagggc gtcagaaatg tagtcaccgt tcaggttcat acaggcgata
     5581 acatcatatt cagccggacg cagcaggatc tgttgcagga atgcatcagc gatcacgtct
     5641 ttaatgacga tctctttgcc agtgttcggg tttttaactt tcagccacgg gccaccgtcg
     5701 atcagttcac cgccaaactc ttcacgcgcc agctggtagc cccagtcttt aaacgctcct
     5761 tcggtgaact tcatgatgtt gcctttgtgc accagagtca cagagtcacg atcgttagca
     5821 attgcgtatt cgatcgctgc acgaaccaga cgtttggtgc cttcttccga acacggctta
     5881 ataccgatac cacaatgttc cgggaagcga attttcttca cccccatctc ttcacgcagg
     5941 aatttaatta ctttctcggc gtcggcagag tctgctttcc attcgatacc cgcataaatg
     6001 tcttccgagt tttcacggaa aataaccata tcggtcagtt cagggtgttt aaccgggctt
     6061 ggagtgccct gatagtaacg taccggacgc aggcagatgt agagatccag ttcctggcgc
     6121 agggcaacgt tcagagagcg aataccgcca ccaacaggag tggtcagcgg acctttaatg
     6181 gcaacgcgat attcacgaat cagatcaagg gtttcagcag gcagccagac atcctgacca
     6241 taaacctgtg tggatttttc accggtgtaa atttccatcc aggagatttt acgctcgcct
     6301 ttataggctt tctcgactgc agcgtcgacc actttcagca tggctggggt tacatctaca
     6361 ccgattccat caccttcaat gtaagggata atcggatttt caggaacgtt gagtttgccg
     6421 ttttgcaggg tgatcttctt gccttgtgcc ggaacaacta ctttactttc cattcacctc
     6481 tccttcgagc gctactggtt tgctcgtctg ccaccacgtt gcatatgcgt ttgcgtcctg
     6541 cgatacggat gctttagagc aattttttgt taatgatttg taattggctt gtcaatacta
     6601 ccctattgtt tgccgtcatg aaagatgcgc gttattaggc tataatgcgg caattcataa
     6661 tctctgaaaa taccatgcaa aaaacttctt ttagaaatca ccaggttaag cgattcagct
     6721 cgcaacgttc taccaggcgt aaacctgaaa accagcccac gcgtgtgatc ctgttcaata
     6781 aaccctacga tgttcttccg cagttcaccg atgaagccgg acgcaaaaca ttaaaagaat
     6841 tcatcccggt tcagggcgtt tatgcagcag gtcgccttga ccgcgatagc gaagggttgc
     6901 tggtgctgac caataacggc gcgttgcagg cgcgtttaac ccagccgggt aaacgcaccg
     6961 gaaaaatcta ttatgtgcag gtggaaggta ttcccacaca agacgcactt gaagccttgc
     7021 gcaatggcgt aaccttaaat gatggcccta ccctgcccgc aggcgcggag ctggttgacg
     7081 aacccgcgtg gttatggccg cggaatccac caattcgtga acgcaaaagt attcccacca
     7141 gctggctgaa gatcacctta tatgaaggac gtaatcgcca ggtgcgccgc atgaccgccc
     7201 atgttggttt ccccacgctg cgactgattc gctatgcgat gggagattac tctttggata
     7261 atcttgccaa tggcgaatgg cgagaagtga cagattaagg atgcaaaatg tttaaaccgc
     7321 acgttaccgt tgcttgcgtg gtgcacgcag aaggcaaatt tttagtcgtt gaagagacga
     7381 ttaatggtaa agcgttatgg aaccaacctg ccgggcatct ggaagccgat gaaaccttag
     7441 tggaagccgc cgcccgtgaa ctgtgggaag aaaccggcat cagcgcgcag ccgcaacact
     7501 ttattcgtat gcatcagtgg attgcgccag ataaaacgcc gtttttgcgt ttcctctttg
     7561 ccattgagct tgagcaaata tgcccgaccc agcctcatga cagcgatatc gactgctgcc
     7621 gttgggtcag cgccgaagaa attttacagg cgtcaaatct tcgttcgccg ctggtggcgg
     7681 aaagtattcg ttgttatcaa agcgggcaac gttatccgct ggagatgatt ggcgatttta
     7741 actggccttt tacaaagggt gtcatctaaa aggctgcgtg atagaatacg ccgccttgaa
     7801 gttcaatgtc gtgagtgatc caatgtctga aaccgcaaaa aaagtaatcg tcggcatgtc
     7861 cggcggtgtc gattcctccg tttctgcctg gctgttgcaa caacagggat atcaggtcga
     7921 aggcctgttt atgaagaact gggaagaaga cgacggtgag gaatattgca cagcggcagc
     7981 ggatctggct gatgcccagg ctgtctgcga caagctcggc attgaactgc acaccgttaa
     8041 ctttgctgcc gagtactggg acaacgtctt cgaactgttc cttgccgaat ataaagccgg
     8101 tcgcacgccg aatccggata ttctgtgcaa caaagagatc aaatttaaag ccttcctcga
     8161 atttgccgcc gaagatttag gtgccgatta tatcgctacc ggtcattacg tacgtcgggc
     8221 cgatgtcgat ggcaagagcc gcctgctgcg tggtctggac agcaataaag accagagcta
     8281 cttcctttat acgctcagcc atgagcagat tgcgcaaagc ctgttcccgg tcggcgaact
     8341 ggaaaaaccg caggtgcgta agattgctga agatcttggt ctggtcaccg cgaagaaaaa
     8401 agactctacc ggcatttgct tcattggcga acgtaaattc cgcgagttcc tgggccgtta
     8461 tctcccggcg caaccgggca aaatcattac cgtcgatggc gatgaaattg gtgagcacca
     8521 ggggctgatg tatcacactc tcggtcagcg taaaggtctg ggtatcggtg gcaccaaaga
     8581 aggtaccgaa gaaccgtggt atgtggtgga caaagacgtc gaaaacaaca ttctggttgt
     8641 cgctcagggc catgaacacc cgcggctgat gtctgtcggg ttgattgccc agcagttgca
     8701 ctgggtcgat cgcgaaccat tcaccggcac tatgcgttgc acggtaaaaa cccgctatcg
     8761 ccagaccgat atcccttgca ccgtcaaggc gctggacgat gatcgcattg aagtgatttt
     8821 cgatgaaccg gttgccgccg tgacgccggg ccagtctgcc gtcttctata acggtgaagt
     8881 gtgcctcggt ggcggtatta ttgagcagcg tctgccgctg ccggtctgat tattatcttt
     8941 acttaaaaca aggaagcagt gaacgtggca aagaattact atgacatcac cctcgccctg
     9001 gccggtattt gtcagtcggc acgcctggtg caacaactcg ctcaccaggg gcattgtgat
     9061 gccgatgcgc tacacgtctc actcaacagt attattgata tgaaccccag ctcgacgctg
     9121 gcggtttttg gcggtagcga agccaacctg cgcgtcgggc tggaaaccct gctcggcgtg
     9181 ctcaatgcca gcagtcgcca gggcttaaac gccgaattaa cccgctacac actcagcttg
     9241 atggtgcttg agcgcaaact ctcctcagcg aaaggcgcgc tcgacactct gggcaaccgg
     9301 atcaacggcc tgcaacgcca gctcgaacac ttcgatttac agtccgaaac gctgatgagc
     9361 gcgatggctg ctatctatgt tgatgtgatt agcccgcttg gcccgcgcat tcaggtcacc
     9421 ggttcccctg ctgtactgca aagcccacaa gtgcaggcga aagttcgcgc aaccctgctg
     9481 gcaggcattc gcgccgccgt gctctggcac caggtcggcg gcggacgtct gcaactgatg
     9541 ttttctcgta atcgcctgac cactcaggca aaacaaattc ttgctcattt aaccccggag
     9601 ttgtgatcta tggaattatc ctcactgacc gccgtttccc ctgtcgatgg acgctacggc
     9661 gataaagtca gcgcgctgcg cgggattttc agcgaatatg gtttgctgaa attccgtgta
     9721 caagttgaag tacgttggct gcaaaaactg gccgcgcacg cagcgatcaa ggaagttcct
     9781 gcttttgctg ccgacgcaat cggttacctt gatgcaatcg tcgccagttt cagcgaagaa
     9841 gatgcggcgc gcatcaaaac tatcgagcgt accactaacc acgacgttaa agcggttgag
     9901 tatttcctga aagaaaaagt ggcggagatc ccggaactgc acgtggtttc tgaattcatc
     9961 cactttgcct gtacttcgga agatatcaat aacctctccc acgcattaat gctgaaaacc
    10021 gcgcgtgatg aagtgatcct gccatactgg cgtcaactga ttgatggcat taaagatctc
    10081 gccgttcagt atcgcgatat cccgctgctg tctcgtaccc acggtcagcc agccacgccg
    10141 tcaaccatcg gtaaagagat ggcaaacgtc gcctaccgta tggagcgcca gtaccgccag
    10201 cttaaccagg tggagatcct cggcaaaatc aacggcgcgg tcggtaacta taacgcccac
    10261 atcgccgctt acccggaagt tgactggcat cagttcagcg aagagttcgt cacctcgctg
    10321 ggtattcagt ggaacccgta caccacccag atcgaaccgc acgactacat tgccgaactg
    10381 tttgattgcg ttgcgcgctt caacactatt ctgatcgact ttgaccgtga cgtctggggt
    10441 tatatcgccc ttaaccactt caaacagaaa accattgctg gtgagattgg ttcttccacc
    10501 atgccgcata aagttaaccc gatcgacttc gaaaactccg aagggaatct gggcctttcc
    10561 aacgcggtat tgcagcatct ggcaagcaaa ctgccggttt cccgctggca gcgtgacctg
    10621 accgactcta ccgtgctgcg taacctcggc gtgggtatcg gttatgcctt gattgcatat
    10681 caatccaccc tgaaaggcgt gagcaaactg gaagtgaacc gtgaccatct gctggatgaa
    10741 ctggatcaca actgggaagt gctggctgaa ccaatccaga cagttatgcg tcgctatggc
    10801 atcgaaaaac cgtacgagaa gctgaaagag ctgactcgcg gtaagcgcgt tgacgccgaa
    10861 ggcatgaagc agtttatcga tgatctggcg ttgccagaag aagagaaagc ccgcctgaaa
    10921 gcgatgacgc cggctaacta tattggtcga gctatcacga tggttgatga gctgaaataa
    10981 acctcgtatc agtgccggat ggcgatgctg tccggcctgc ttattaagat tatccgcttt
    11041 ttattttttc actttacctc ccctccccgc tggtttattt aatgtttacc cccataacca
    11101 cataatcgcg ttacactatt ttaataatta agacagggag aaataaaaat gcgcgtactg
    11161 gttgttgaag acaatgcgtt gttacgtcac caccttaaag ttcagattca ggatgctggt
    11221 catcaggtcg atgacgcaga agatgccaaa gaagccgatt attatctcaa tgaacatata
    11281 ccggatattg cgattgtcga tctcggattg ccagacgagg acggtctgtc actgattcgc
    11341 cgctggcgta gcaacgatgt ttcactgccg attctggtat taaccgcccg tgaaagctgg
    11401 caggacaaag tcgaagtatt aagtgccggt gctgatgatt atgtgactaa accgtttcat
    11461 attgaagagg tgatggcgcg aatgcaggca ttaatgcggc gtaatagcgg tctggcttca
    11521 caggtcattt cgctcccccc gtttcaggtt gatctctctc gccgtgaatt atctattaat
    11581 gacgaagtga tcaaactgac cgcgttcgaa tacaccatta tggaaacgtt gatacgcaat
    11641 aatggcaaag tggtgagcaa agattcgtta atgctacaac tctatccgga tgcggagctg
    11701 cgggaaagcc ataccattga tgtactgatg ggacgtctgc gcaaaaaaat tcaggcacaa
    11761 tatccgcaag aagtgattac caccgttcgc ggccagggct atctgttcga attgcgctga
    11821 tgaaaaaatt actgcgtctt tttttcccgc tctcgctgcg ggtacgtttt ctgttggcaa
    11881 cggcagcggt agtactggtg ctttcgcttg cctacggaat ggtcgcgctg atcggttata
    11941 gcgtcagttt cgataaaact acgtttcggc tgttacgtgg cgaaagcaat ctgttctata
    12001 cccttgcgaa gtgggaaaac aataagttgc atgtcgagtt accagaaaat atcgacaagc
    12061 aaagccccac catgacgcta atttatgatg agaacgggca gcttttatgg gcgcaacgtg
    12121 acgtgccctg gctgatgaag atgatccagc ctgactggct gaaatcgaat ggttttcatg
    12181 aaattgaagc ggatgttaac gataccagcc tcttgctgag tggagatcat tcgatacagc
    12241 aacagttgca ggaagtgcgg gaagatgacg acgacgcgga gatgacccac tcggtggcgg
    12301 taaacgtcta cccggcaaca tcgcgaatgc caaagttaac cattgtggtg gtggatacca
    12361 ttccggtgga gctaaaaagt tcctatatgg tctggagctg gtttatctat gtgctctcag
    12421 ccaatctgct gttagtgatc ccgctgctgt gggtcgccgc ctggtggagt ttacgcccca
    12481 tcgaagccct ggcaaaagaa gtccgcgaac tggaagaaca taaccgcgaa ttgctcaatc
    12541 cagccacaac gcgagaactg accagtctgg tacgaaacct gaaccgattg ttaaaaagtg
    12601 aacgcgaacg ttacgacaaa taccgtacga cgctcaccga cctgacccat agtctgaaaa
    12661 cgccactggc ggtgctgcaa agtacgctgc gttctctgcg tagtgaaaag atgagcgtca
    12721 gtgatgctga accggtaatg ctggagcaaa tcagccgcat ttcacagcaa attggctact
    12781 acctgcatcg tgccagtatg cgcggcggga cattgcttag ccgcgagctg catccggtcg
    12841 ccccactgct ggacaatctc acctcggcgc tgaacaaagt gtatcaacgc aaaggggtca
    12901 atatctctct cgatatttcg ccagagatca gctttgtcgg tgagcagaac gattttgtcg
    12961 aggtgatggg caatctgctg gataatgcct gtaaatattg cctcgagttt gtcgaaattt
    13021 ctgcaaggca aaccgacgag catctctata ttgtggtcga ggatgatgga cccggtattc
    13081 cattaagcaa gcgagaggtc attttcgacc gtggtcaacg ggttgatact ttacgccctg
    13141 ggcaaggtgt agggctggcg gtagcccgcg aaatcaccga gcaatatgag ggtaaaatcg
    13201 tcgccggaga gagcatgctg ggcggtgcgc ggatggaggt gatttttggt cgccagcatt
    13261 ctgcgccgaa agatgaataa atatgtccat acttcacgca ttacgttaag catccgttat
    13321 aatcggttgc agataccagc ctgtggatgc ttaacatgga ataccaactc actcttaact
    13381 ggcccgattt tcttgaacgt cactggcaga aacgcccggt ggtgttaaaa cgcggcttta
    13441 ataattttat tgacccgatc tctccagacg agttggcggg tctggcgatg gaaagcgaag
    13501 tcgacagtcg actggtcagt caccaggatg gcaaatggca ggtcagccac ggtccgttcg
    13561 aaagctacga tcatctcggt gaaactaact ggtcattgtt agtgcaagca gtaaatcact
    13621 ggcatgagcc gaccgccgcg ctgatgcgac cgttccgtga actaccggac tggcgtattg
    13681 atgatctgat gatctctttt tctgtacctg gcggcggcgt cggcccgcat ctcgatcagt
    13741 acgacgtgtt tatcattcag ggtaccggac gtcgtcgctg gcgagtgggc gaaaagctgc
    13801 aaatgaaaca gcactgccca cacccggatc tgttacaggt cgatccgttc gaagccatca
    13861 tcgatgaaga gctggagcct ggcgatattc tttatattcc gccaggattc ccgcatgaag
    13921 gctacgcgct ggaaaatgcg atgaactatt ccgtgggttt tcgcgcgcca aatacgcggg
    13981 aattaattag cggatttgcc gattatgtgc tgcaacgtga actgggcggc aactactaca
    14041 gcgatcctga tgttccacct cgcgctcatc ctgcggacgt tctgccgcaa gagatggata
    14101 aactgcgtga gatgatgctc gaattgatca accagccgga acactttaag caatggtttg
    14161 gcgagtttat atcccagtca cgtcatgaac tggatatcgc gccgccagag ccgccttatc
    14221 agccggatga aatctacgat gcgctgaaac aaggtgatgt gctggtgcgc cttggtggtc
    14281 tgcgcgtatt gcgcattggc gacgacgtgt atgccaatgg tgagaagatc gattccccgc
    14341 accgtccggc actggatgca ctcgccagca acattgcgct gactgcggag aattttggcg
    14401 atgcgctgga agatccgtca ttcctcgcga tgctcgcggc gctggtcaat agcgggtatt
    14461 ggttcttcga agggtaagtt tggatttaag ccggatgcgg catcacatgc cgcatccctt
    14521 ttcgcttact tccgttgcgc cgttaactcg gcaatacgga cgatcacctg caccgctttt
    14581 tccatacctt ccagagtcac aaactcatgc ttaccatgat agttgtaacc gccagtgaac
    14641 aggttcgggc acggtaatcc cataaacgac aactgcgcgc cgtcggtacc gccgcggatc
    14701 ggtttcagtt ccggttcaat atcgcagtca cgcatcgcct gctgggcgat atcgagaata
    14761 tgcggatgct caaccacttt ctcgcgcata ttgtagtaac tgtcttcaat caccagttca
    14821 atgtagcaat caggatgtaa ccctttgccc acttttttgg cgatctccat cattttacgt
    14881 ttacgcgctt caaactgttt acggtcgaaa tcacggatga tgtagtgcat atcggctcgg
    14941 tcaacggtgc ctttcatgct cgccaggtga tagaaacctt catagccttc tgtcatttcc
    15001 gggctttcat ccgccggaac ttccgcatga atacgtgccg ccagcgacag cgcatttacc
    15061 atcactcctt tcgccgtgcc cggatgaaca ttgttaccga caattttgat attgaccgat
    15121 gcggcgttga agttttcaaa ctccagttcg cctacgccac caccgtcaac agtgtaagcc
    15181 cagcgggcat cgaaggcatc aacatcaaaa tgtttcgccc ctttgcccac ttcttcatcc
    15241 ggggtaaagg cgacgcgaat atcaccatgc ggaatgtttt tctgttgcaa taccgccagc
    15301 gcggtcatga tttctgcaat accggctttg tcatcggcac ctaacaaggt tttaccatcg
    15361 gtggtaatca acgtctgacc cagtagctga tgcagcaccg ggaacataac cggtgataaa
    15421 acttcatcgc cgatacccag cgcaatatcg ccaccgcgat agttttcaac aatttgcgga
    15481 ttaacatttt tgccgctgca atccggtgag gtatccacat gagaaataaa gccaatcgcc
    15541 gggatatcgc cagggacgtt agccggtaac gtcgccatca aagtgccctt ctcacttaag
    15601 gtcacattga taagccccat ttcttcgagc tgctctttca gcagatgcaa taacttccat
    15661 tggccttccg tgctgggaac ctgtctcacc cctgcttttg attgggtatc cagagacacg
    15721 tagttcaaaa atcgctcaag tagtttatcc atgtagtcac cctcactttt tgtgacaaca
    15781 ttattaagaa gcaagaaaag acaaatattg cgtcaggtca cttttacccc tgcaagcggg
    15841 aatatttatc agcattacct taatgaatat aaagctaagc cagtaattca caacaaagat
    15901 tggcgattca agcggtttgc cgtagaatta ccgccctcat taagagtcac caaggtggtt
    15961 aaccacaaac cccgcatcgg taagccatcc gttgcgttta catgggacag agtaaaaaat
    16021 tgaataaaca accgagttcg ctttcaccgc tggtgcaatt ggcgggaatt cgcaaatgct
    16081 ttgatggtaa agaggtcatt ccccagctgg atctgactat caacaatggc gagttcctca
    16141 cgctgcttgg cccttctggc tgcggtaaaa caaccgttct tcgcctgatt gcaggtctgg
    16201 aaactgttga ttccggacgc atcatgctgg ataacgagga catcacccac gttccggcgg
    16261 aaaaccgcta tgtgaacact gttttccaaa gctacgcact tttcccccac atgaccgtgt
    16321 tcgaaaatgt ggcctttggg ttgcgcatgc aaaaaacccc cgctgctgaa attacgcccc
    16381 gcgtgatgga agccctgcgg atggtgcaac tggaaacctt cgctcaacgt aaaccgcatc
    16441 agctctctgg tggtcaacag caacgcgtcg ccattgctcg cgcggtggtt aacaagcctc
    16501 gtctgttgtt gctggatgag tcgctctcag cgctggatta caaactgcgt aagcaaatgc
    16561 agaacgagct gaaagcgtta cagcgtaagc ttggtattac cttcgtcttt gtcactcacg
    16621 atcaggaaga agcgctcacc atgtcagaca ggattgtggt gatgcgcgag ggtcgtattg
    16681 agcaagatgg tacaccgcgc gaaatttacg aagagccgaa aaacctgttt gttgccggct
    16741 tcattggcga aatcaatatg tttaacgcca ctgtcatcga acgtctagac gagcagcgcg
    16801 tacgcgccaa cgttgaaggc cgcgaatgta atatctacgt taacttcgcc gttgaaccgg
    16861 ggcaaaaact gcatgttctg ctgcgcccgg aagacttacg tgttgaagag attaacgacg
    16921 acaaccacgc tgaagggctg attggttacg ttcgtgagcg taactacaaa ggcatgacgc
    16981 tggagtcggt tgttgaactg gaaaatggca agatggtgat ggtcagcgaa ttcttcaatg
    17041 aagacgaccc tgactttgac cactctctcg accaaaaaat ggccattaat tgggtagaaa
    17101 gctgggaggt cgtactggct gatgaagaac acaagtaagt tccagaatgt agtgattgtc
    17161 actattgtcg gttggcttgt gttgtttgtc tttctgccca acctgatgat cattggcacc
    17221 agctttttga cccgcgacga cgccagtttc gtcaaaatgg tctttacgct ggataactac
    17281 acgcgtctgc tcgatccgct ctattttgaa gtgctattgc attcgctgaa tatggcgctg
    17341 atcgccaccc tcgcctgcct ggtgctgggc tacccgtttg cctggtttct ggcgaagctg
    17401 ccacacaagg tgcgtccgct gctgctgttt ctgctgattg ttcccttctg gaccaactca
    17461 ctgattcgca tctacgggct gaaaattttc ctcagcacca aaggctatct caacgagttt
    17521 ttgctctggc tgggcgttat cgacacacca atccgtatca tgttcacgcc cagtgcggtg
    17581 attatcggtc tggtttacat tctgctgccg tttatggtga tgccgctgta ctccagtatt
    17641 gaaaaactgg ataagccgtt gcttgaggcg gcgcgcgatc tcggtgccag caagttgcag
    17701 acctttatcc gcatcattat tccactgacg atgccgggaa ttattgccgg atgtctgctg
    17761 gtgatgctgc cagcgatggg cctgttctat gtatccgacc tgatgggcgg tgcgaaaaac
    17821 ctgctgatcg gtaacgtcat caaggtacag ttcctcaata ttcgtgactg gccatttggt
    17881 gcagccacca gcattacgct gactatcgta atgggcctga tgttgctggt ttactggcgc
    17941 gcttctcgtt tgctgaataa gaaggtggaa ctggaatgat cggtcgactg cttcgcggcg
    18001 gttttatgac cgctatctac gcgtacctgt atatcccaat cattattttg attgtgaact
    18061 cctttaacag ctcgcgcttt ggcatcaact ggcagggttt taccaccaaa tggtatagcc
    18121 tgctgatgaa caacgacagc ctgttacagg cagcgcagca ttcactaaca atggcggtgt
    18181 tttcggcgac atttgctacg cttatcggtt cactgacggc agttgcgctg taccgttatc
    18241 gctttcgtgg taagccgttc gttagcggaa tgctgtttgt ggtgatgatg tcaccagata
    18301 tcgtgatggc gatttcgctg ctggtactgt ttatgctgct gggaattcag cttggcttct
    18361 ggtcgctgct gttctctcat atcaccttct gcctgccatt tgtggtggtg acggtgtatt
    18421 cgcgcctgaa aggttttgac gtgcggatgc tggaagcggc gaaagatctc ggtgccagcg
    18481 aatttaccat tctgcgaaaa atcatactgc cactggcaat gccagcggtg gcggcgggct
    18541 gggtgttaag ctttaccctg tcgatggacg atgtggtagt ttcttcgttc gtcaccggac
    18601 caagttatga aattctgccg ttaaaaattt attcgatggt caaagtcggc gtatcgccgg
    18661 aagttaacgc gctggcaacc atattactgg tgctgtcgct ggtgatggtt attgccagtc
    18721 agcttattgc tcgtgataaa acgaaaggta acacagggga cgttaaatga aaaaatggtc
    18781 acgccacctg ctcgcggcgg gtgctctggc actgggcatg agcgccgctc acgccgatga
    18841 caacaacacg ctgtatttct acaactggac cgagtacgtg ccgccaggac tgcttgaaca
    18901 gttcaccaaa gaaaccggta ttaaggttat ctattcgact tacgagtcga acgaaaccat
    18961 gtacgccaag ctgaaaacgt acaaagacgg tgcctacgac ctggtggttc cttcaaccta
    19021 ttacgtcgat aaaatgcgta aagaagggat gatccagaag atcgacaagt cgaagttaag
    19081 caatttcagc aacctcgatc cagacatgct caacaagccg tttgacccga ataacgacta
    19141 ctccattccg tatatctggg gggcgacggc gattggcgtt aacggtgatg cggtagatcc
    19201 gaaatccgtt accagctggg ccgatctgtg gaagcctgag tacaaaggca gcctgctgct
    19261 gaccgacgat gcccgtgaag tgttccagat ggcgctgcgt aagctgggct actccggtaa
    19321 caccaccgat ccgaaagaga ttgaagctgc atataacgag ctgaaaaaac tgatgccaaa
    19381 cgtggcagcc tttaactccg ataacccggc taacccgtac atggaaggcg aagttaacct
    19441 cggcatgatc tggaacggtt ctgctttcgt tgcacgccag gcgggtacgc caattgacgt
    19501 ggtgtggccg aaagaaggcg gcattttctg gatggacagc ctggcgatcc cggcaaatgc
    19561 caaaaacaaa gaaggcgcgc tgaaattgat caacttcctg ctgcgcccgg atgtggcaaa
    19621 acaggttgct gaaactatcg gttatccaac gccaaacctt gcggcgcgta agctgttaag
    19681 tccagaagtg gcgaacgata agacgctcta cccggatgct gaaaccatta aaaatggcga
    19741 atggcagaat gacgttggcg cagccagcag catttatgaa gagtattatc agaagctgaa
    19801 agcaggacgt taattcagca agcgggcggt ggtattaccg cccgtttttt agttagcaag
    19861 gatgtggcta atgagtcagg acagtaaagt tttatcccgc gcttttctcg gtatcggtct
    19921 gatattgctc gttatttctg caatcatctt ttattaccat tttactttta ccaaaagcgc
    19981 ggttcataca gaaggaatca ttgttgatgc cgtttggtac aacaatcact caaacgacgt
    20041 cgatgataat ggttcctggt atccagtggt tgcctttcgc cctacgccag attacacgct
    20101 aattttcaat tcgaatatcg gtagtgattt ttatgaagat agcgaaggtg acagagtcaa
    20161 tgtctattat ccgccagggc atccagaaca agccgaaata aacaatccct gggttaattt
    20221 ttttaaatgg ggctttgttg gcatagcagg cattattttc ggtagtgtcg gtctgataat
    20281 cacgctcccc tccaccaaaa aatctcgcag gaaacggaag tcccgcccat gaaaaaattc
    20341 attatactgc tcagtttact gatactcctg cctctggtag caaccagtaa gccattgatt
    20401 ccaataatga agacactttt tacggatgtt accggtactg taccggatgc tgaagagatc
    20461 gcacgtaaag ccgagctttt tcgccaacaa acgggaattg cactgtttat tgtgatttta
    20521 cccgacatta ataatgaagc cagtctcaga caaaatggta aagcgatgct ggcgcatgcg
    20581 tcatcttcat tgagtaatgt aaaaggaagt gttctgttac tatttactac ccgcgaacca
    20641 cggttaatta tgatcaccaa cggccaggtt gaaagtggtc tggacgataa acatctcggc
    20701 cttctgatag aaaatcacac gctggcttat ttaaatgcag atctctggta tcagggaatc
    20761 aataatgcat tggctgttct acaagcacag atattaaaac aatcgacgcc gccactaacg
    20821 tattatccgc atccaggaca acagcatgaa aatgctcccc ccggcagcac caacacatta
    20881 ggttttatcg cctgggctgc gacgtttatc ttattttcca ggatctttta ctacacgact
    20941 cgttttattt atgcgttaaa atttgcagtg gcgatgacta ttgccaatat gggttatcag
    21001 gctttatgtt tgtatatcga taatagcttt gcgattacca gaatatcgcc actctgggca
    21061 gggctaattg gtgtctgtac gtttattgcc gcattgttat tgacgagtaa acgataaaag
    21121 aggccggaaa agtgcgcgtg tattatgccg gatggggcgc gaacgcctta tccggcccac
    21181 aaaacccgca aattcaatta attgcgtccc cttgcaggcc tgataagcgt agtgcatcag
    21241 gcaatgcttc ccgcttttaa tcccttcagc aacttttcaa caaactcagg caccacctgg
    21301 cttgccgggc cgtaatattt ctcggcaaat tcattaccaa cctgactcgg ttcaagattc
    21361 agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc cgccggataa
    21421 acatgcccgg atgtgccaat ggcaatgaaa atatcggcca tcgacaacgc catataaatt
    21481 tcatccatgc cgagtggcat ttcgccaaac cacactacat gcgggcgcaa gggtgccgga
    21541 aactggcaac aatggcattt atcttctggg gtaacgtctc ctgtccagtc gagaacctga
    21601 ccactttgtg aacaacgaac tttcagcagt tccccatgca tatgaatcac attggtatta
    21661 cctgcgcgtt catgcaggtt gtcgatattc tgcgtcacca gcaaaaagcg atcgccgagg
    21721 gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc tggctgctgc
    21781 agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg atcgaaacct
    21841 tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc ggcgcgaaag
    21901 gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac tcttggtttt
    21961 tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa acgctcgcgc
    22021 aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga cagcatagca
    22081 acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc tcccgcatca
    22141 ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg acgaggcagc
    22201 ctgtccgcca gttgcgctgt gattgccggg aaattcgata agccaccacc aatgatgacc
    22261 aggtcagggt caacaatggt caggatattt cccagacaaa ccgctaataa atccagataa
    22321 cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat aattttggga
    22381 gcctgcaacg gttgatgata atagtgttga tacagccacg caaaaccgcg accagacaga
    22441 taattttcaa tgcagccatg ctgaccacag ccgcagcggc gtaacgggaa atccaacccc
    22501 atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt aatgtagctt
    22561 ttaccggtaa tcggtttgcc gttgaaaatc agcccgccgc caacgccggt gccgagaatc
    22621 aaccccatca ccagtggata ttgagtaaat tcatcatccc aggcttctga aagggcaaaa
    22681 cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag gtcggcacgc
    22741 agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc ttctgtttcc
    22801 ggcattcccg gaataccgat gccgacagaa cctttacagc caaaacgttg gtcagcttca
    22861 gctaccagct cacacactgc atctaaaaat gcgtcatagc tgtcacgcgg tgtcggcacc
    22921 cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat ttttgttcca
    22981 ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc tttactggcc
    23041 gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc aacttgccaa
    23101 aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt gcaattccga
    23161 tggcaggaaa tcaataaaat agatatcgct ggagaggaac tgatgaccga tcaacttttc
    23221 aatccactca ataatcgggg taagttgcag tgaaaccact acgccgataa tcacaccaca
    23281 caggctgccg aacagccctg ccagcaatcc ataccagaca aagatggcgc gaattaaacc
    23341 atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt tcaccgccat
    23401 cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca tcgccagata
    23461 cataatggcg cggatcattt ggatatcgcg atacatatag ccgtaagtac caatccagct
    23521 tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct tattggcgtt
    23581 gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat caagatattg
    23641 ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac tcaactgcaa
    23701 aataccggca acgtgcaaac gcacacgttt tggctgcatc agtttatgct cgggattcga
    23761 gttggggatc ataatcgaca cccaatcccc ctgcttcact ttcagcgcat ccgccacgcc
    23821 tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccacgcat cgccctggac
    23881 aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct tcacctggat
    23941 tgcgcgaaga ttcgcgccac tttccaccag cccggtgaaa ttgatatacg gcgcggcagc
    24001 ggcaataccc ggcacttttt gcacgtgatc cagtgcttcc tgccagttag tccacggctg
    24061 atccaccgcc tcgatttcac catgcggcac caccgccaga atacggttgt tcagttcgcg
    24121 ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga gggcaatgcc
    24181 aatggtagaa atgacggaga tcagcgacac catgccgccg cgccgccgtc cgcgactaaa
    24241 acgcaggcca attaataacg ataaaggcat cgccattact ccgcccccat caggctcagt
    24301 tccgccgtca gacgaccatc acgcatttct aactggcggc tcatacgttt cgccagttgc
    24361 aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc cccaagcaac
    24421 tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggttcatc cgccagtacc
    24481 aggcgcgggt tattcaccag cgcacgggca atcgccacac gctggcgttc gccgccagaa
    24541 agttcagatg ggcggtgatt cgcacgatga tccagcccca ccgcttttaa catctcaagt
    24601 gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagcggcat agccacgttt
    24661 tccagggcgg taaaatccgg cagcaggtgg tgaaactgat aaataaagcc cagcttctgg
    24721 ttgcgcagtt cagctttcgc tgccgacgac agtttgctca ttggctgacc attaaaaatc
    24781 acatcgccgg aggttggcgt atccagcccg cccagcaggt gcagcaaggt acttttaccg
    24841 gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa actgacattg
    24901 tgcagcacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt gtcgcattgc
    24961 aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag cgcgccatga
    25021 agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa caatgacctg
    25081 taaaggttcg atagccaccg gcagcgccgc gccatcaagc aggacgccga ttatcggcat
    25141 cagattattt aactggctgg caagcagggc gccaagcgcc gctccgagga tcgcaccgat
    25201 aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg gagttaagcc
    25261 ttgcgtttgc aggatcgcta cttcgccctg cttctccatt accatcagcc ctagtgaggt
    25321 aataatgtta aacgccgcaa cggcgacaat caggctcagc agtaaaccca tcatattttt
    25381 ttccatgcgt acggcctgga acaactcgcc tttacgatca cgccagtcct gccatttgct
    25441 gccttcaggc agtttttgct gacttaatga gtcgactttc agcggctcat ccagccacaa
    25501 acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat cctcaatatt
    25561 caccagcatt tcattgccat cgacttcact gttggcggcg aaagtgccaa tcacattgaa
    25621 caggcgctga cttggaatac gccccatcgg cgtgaactgg ctggcagatg gtaccatcat
    25681 gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc cgaggatgac
    25741 attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg gcgttagtgg
    25801 atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc gcgcgctttg
    25861 cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca gtttgactgc
    25921 cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa ttgcctgtgg
    25981 catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca ctgacaatac
    26041 tgtgaccagc gccatcactc cgagggtaat gccgatggta gaaagccagg agacgaaacg
    26101 accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata gagcgacagg
    26161 ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa gcgaaagcag
    26221 gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag ccccagtaaa
    26281 tcaatcggct aaattgccat gttttatcat caatcttgcc acctgatctt aatctgaaaa
    26341 atcatccaat acagacagat acccattaca cctcgggtca taacccgcag gatcaaccgc
    26401 atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg gattaaccag
    26461 atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac cttttatccg
    26521 catctgacca cttttcagca tccgttatcg gaagtcctga gcaaatgctg gatctatttc
    26581 aatctttacc ttgccccctt tcgtatgccg gtttttttct ttatatccgg ctatttgatt
    26641 cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat ctggaacatc
    26701 ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc gctgaaccag
    26761 tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc cgattccacc
    26821 ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct gtatgcgtta
    26881 attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgctact attcgccttg
    26941 tttgtactgt tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg aatgaacagt
    27001 gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac aataatgacc
    27061 tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct taccgttctg
    27121 gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc gattgtggtg
    27181 atcatgaagc tgttttatca atacgagcaa cgattcggta tgcgctccac cagcctgctg
    27241 aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct ggttgaaata
    27301 ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca agtcgaactg
    27361 acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc gggcttgctg
    27421 gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc ttcactgccc
    27481 gctgccgtca gttactcccg ctaagccatc gccccagccg gggcgattca atttgctaaa
    27541 ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc catatgttga
    27601 ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt atacgctgcc
    27661 cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct gtgcaacgct
    27721 ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac cagatatgca
    27781 aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg tgatgaatct
    27841 ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca ttatctcctc
    27901 gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga ttgttccggt
    27961 gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg cgctggtgat
    28021 gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca gcgccggtta
    28081 tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg cgttgctgga
    28141 tcttttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg atgaaatcga
    28201 cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag aagcgatcaa
    28261 tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt tccgcagcca
    28321 gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc aagtgagtaa
    28381 aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg aaccactgcc
    28441 gccgctgttc agttatttcc ctgccaatac cttgctggtg aatactggcg atctggaaac
    28501 cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg gcgtcgatcc
    28561 gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc tcttctcaga
    28621 gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa aagccgcgaa
    28681 tgccaattta ggtttccaga aactgccaga cctggccgtt caggcgcaac aaaaagcgcc
    28741 gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt tctcggtaga
    28801 aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa ttgctccgca
    28861 acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga ttggcgctgc
    28921 cgaacatggt tttgtcgata cggtgcgtaa tctggcgctg atctgcgaaa gcgatctgct
    28981 cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc ccgatacact
    29041 gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg agcacggtgt
    29101 cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg agtatttgat
    29161 gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc atctgattag
    29221 ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg gcgatgcgtg
    29281 gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg aattgctgga
    29341 tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc gtgagcagta
    29401 tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc aggccattaa
    29461 tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt gcggcgatgt
    29521 tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag ataaccacaa
    29581 gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg acaacttccg
    29641 cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc gcagcgccaa
    29701 agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc tgatcggtac
    29761 gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga ttgtcgatga
    29821 agaacaccgc ttcggggtgc gtcataaaga gcgtattaaa gcgatgcgcg cgaacgtgga
    29881 tattctgacg cttactgcaa cgccgatccc acgtacgctg aatatggcaa tgagcggaat
    29941 gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta aaacctttgt
    30001 ccgtgagtat gacagcctgg tggtccggga ggcgatcctg cgtgaaattt tgcgcggagg
    30061 acaggtttat tatctctaca atgatgtgga aaacattcag aaagccgccg aacggctggc
    30121 agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg agcgcgaact
    30181 ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt gtacaaccat
    30241 tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac gcgcggatca
    30301 cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc atcatcaggc
    30361 atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcgc aaaaacgtct
    30421 tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa cgcacgatct
    30481 ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa tggaaaccat
    30541 cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga aagccggacg
    30601 cgagccgtcg ctggaagatc tcaccagcca gcaaacagag gtcgagctgc ggatgccgtc
    30661 gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct ataaacgtat
    30721 tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta tcgatcgctt
    30781 cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc gccagcaagc
    30841 gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga tcgaatttgc
    30901 cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac agccgcagca
    30961 ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg agcggaaaac
    31021 gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga tcgcttaatg
    31081 gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt tgcacttccc
    31141 tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt acaataacct
    31201 tatatttatt atggattttt ggtgatgatc aaaacgcgtt tttctcgctg gctaacgttt
    31261 tttacgttcg ccgctgccgt ggcgctggcg ctaccggcaa aagccaacac ctggccgctg
    31321 ccgccagcgg gcagtcgtct ggttggcgaa aacaaatttc atgtggtgga aaatgacggt
    31381 ggttctctgg aagccatcgc caaaaaatat aacgtcggct ttctcgctct gttacaggct
    31441 aaccctggcg ttgatcctta cgtaccgcgc gcgggtagcg tgttaacgat cccgttgcaa
    31501 accctacttc cagatgcgcc gcgcgaaggc attgtgatca atattgcgga gctgcgtctc
    31561 tattactacc cgccgggtaa aaattcggta accgtgtatc caataggtat tggtcagtta
    31621 ggtggtgaca cgctgacacc gacaatggtg accaccgttt cagacaaacg tgcaaaccca
    31681 acctggacgc caacggcaaa catccgcgcc cgttataaag cacagggaat tgagttgcct
    31741 gcggtagtgc cggctggacc ggataaccca atgggccatc atgcgattcg tctggcggcc
    31801 tatggcggcg tttatttgct tcacggtacg aacgccgatt tcggcattgg catgcgggta
    31861 agttctggct gtattcgtct gcgggatgac gatatcaaaa cactctttag ccaggtcacc
    31921 ccaggcacca aagtgaatat catcaacact ccgataaaag tctctgccga accaaacggt
    31981 gcgcgtctgg ttgaagtaca tcagccgctg tcagagaaga ttgatgacga tccgcagctg
    32041 ctgccaatta cgctgaatag cgcaatgcaa tcatttaaag atgcagcaca aactgacgct
    32101 gaagtgatgc aacatgtgat ggatgtccgt tccgggatgc cggtggatgt ccgccgtcat
    32161 caagtgagcc cacaaactct gtaaatcctg gataaaaaaa atccccgcga cacggcatgt
    32221 cgcggggaaa aaatcagcag tagcaggcat taatgagggt taatgcttat ttataaatta
    32281 ctgctgttcc atggagggta ttcggaccgg ttacagaagt aatacggaaa gattttgcgc
    32341 ccatctcatc cgctttttgc gccagctgct cttccagcga tcccagattt gtccccgcgt
    32401 tagcactgat tgtaccgact ttttgttggc cttctggcgt tgactgaact tcgacagccg
    32461 caaagctggc aaatgacatg gagcttaaaa tcgccgcagc gatgagggtt tttacgtttt
    32521 tcataatagt ggccttatgc agatgaatga cgtcgcaa
//
LOCUS       DAOFQD010000049        32355 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.049, whole genome shotgun sequence.
ACCESSION   DAOFQD010000049 DAOFQD010000000
VERSION     DAOFQD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.049"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(305..739)
                     /gene="pcoE"
                     /locus_tag="QBF46_002289"
     CDS             complement(305..739)
                     /gene="pcoE"
                     /locus_tag="QBF46_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system metallochaperone PcoE"
                     /protein_id="HDQ6674465.1"
                     /translation="MKKILVSFVAIMAAASSAMAAETMNMHDQVNNAQAPAHQMQSSA
                     EKSAIQGDSMTMMDMSSHDQAAMSHDMMQNSNSAAHQDMAEMHKKMMKAKPGATNETA
                     KSFSEMSEHEKAAAVHEKANNGQSSVVHQQQADKHRSQITQN"
     gene            complement(957..2357)
                     /gene="pcoS"
                     /locus_tag="QBF46_002290"
     CDS             complement(957..2357)
                     /gene="pcoS"
                     /locus_tag="QBF46_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance membrane spanning protein
                     PcoS"
                     /protein_id="HDQ6674466.1"
                     /translation="MRFKISLTTRLSLIFSAVMLTVWWLSSFILISTLNGYFDNQDRD
                     FLTGKLQLTEEFLKTETFRNKTDIKSLSEKINDAMVGHNGLFISIKNMENEKIVELYA
                     KNSVVPAVLLNKSGDILDYMIQTEENNTVYRSISRRVAVTPEQGKSKHVIITVATDTG
                     YHTLFMDKLSTWLFWFNIGLVFISVFLGWLTTRIGLKPLREMTSLASSMTVHSLDQRL
                     NPDLAPPEISETMQEFNNMFDRLEGAFRKLSDFSSDIAHELRTPVSNLMMQTQFALAK
                     ERDVSHYREILFANLEELKRLSRMTSDMLFLARSEHGLLRLDKHDVDLAAELNELREL
                     FEPLADETGKTITVEGEGVVAGDSDMLRRAFSNLLSNAIKYSPDNTCTAIHLERDSDC
                     VNVMITNTMSGQVPANLERLFDRFYRADSSRFYNTEGAGLGLSITRSIIHAHGGELSA
                     EQQGREIVFKVRLLMD"
     gene            complement(2354..3034)
                     /gene="pcoR"
                     /locus_tag="QBF46_002291"
     CDS             complement(2354..3034)
                     /gene="pcoR"
                     /locus_tag="QBF46_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     PcoR"
                     /protein_id="HDQ6674467.1"
                     /translation="MQRILIVEDEQKTGRYLQQGLVEEGYQADLFNNGRDGLGAASKG
                     QYDLIILDVMLPFLDGWQIISALRESGHEEPVLFLTAKDNVRDKVKGLELGADDYLIK
                     PFDFTELVARVRTLLRRARSQAATVCTIADMTVDMVRRTVIRSGKKIHLTGKEYVLLE
                     LLLQRTGEVLPRSLISSLVWNMNFDSDTNVIDVAVRRLRSKIDDDFEPKLIHTVRGAG
                     YVLEIREE"
     gene            complement(3089..4018)
                     /gene="pcoD"
                     /locus_tag="QBF46_002292"
     CDS             complement(3089..4018)
                     /gene="pcoD"
                     /locus_tag="QBF46_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance inner membrane protein PcoD"
                     /protein_id="HDQ6674468.1"
                     /translation="MNDLIMIVIRFLLYLDLMVIFGLPFFQIYGISGVRHETYNLTNF
                     RSFITFAVVTGIILTGINMLLVSNAMSGVTDLRELSIHVIEMVIEETDVGISWIVRLC
                     ALFTTLGALFLYTNKRVLSCLLMTMSGGVALATLAWGGHAVMHDGLHYYLHLLSDLTH
                     LGAAGAWTGALVAFAILLMRRNEHNAQSVIVISDSLAKFATAGTVIVVALILSALVNY
                     LYIAEGNLTPLFNSSWGRILLAKTALFVLMLLLAAANRFHLGPRLEVMVREGNYDRSV
                     ALMRNSILTEFVVAIIILGAVAWLGMLAPSQVS"
     gene            complement(4023..4403)
                     /gene="pcoC"
                     /locus_tag="QBF46_002293"
     CDS             complement(4023..4403)
                     /gene="pcoC"
                     /locus_tag="QBF46_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system metallochaperone PcoC"
                     /protein_id="HDQ6674469.1"
                     /translation="MSILNKAILTGGLVMGVAFSAMAHPELKSSVPQADSAVAAPEKI
                     QLNFSENLTVKFSGAKLTMTGMKGMSSHSPMPVAAKVAPGADPKSMVIIPREPLPAGT
                     YRVDWRAVSSDTHPITGNYTFTVK"
     gene            complement(4443..5333)
                     /gene="pcoB"
                     /locus_tag="QBF46_002294"
     CDS             complement(4443..5333)
                     /gene="pcoB"
                     /locus_tag="QBF46_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance outer membrane transporter
                     PcoB"
                     /protein_id="HDQ6674470.1"
                     /translation="MKRNLKAIPVLVAGLFTSQLSIAAGSVSADPHAGHDMSAMQMPA
                     DENFTEMTSMEPIVTESRTPIPPVTDADRKAAFGNLQGHAIHDSAINYLVLLDQLEWQ
                     RSDNTNNFSWSVNSWIGGDTDRIWLKSEGERSNGETEAAEAQLLWGHAVGPWWDLVTG
                     VRQDFRPASARTWAAVGFQGLALYNFESEITGFVSNGGKAALRLGGEYDVLLTNRLIL
                     QPSYEVNFYSQDDESRGRGRGLTDTELGLRLRYEIRREFAPYIGVSWNQLYGKTSDMA
                     KREGEKDHQVVFLAGARIWF"
     gene            complement(5339..7156)
                     /gene="pcoA"
                     /locus_tag="QBF46_002295"
     CDS             complement(5339..7156)
                     /gene="pcoA"
                     /locus_tag="QBF46_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase PcoA"
                     /protein_id="HDQ6674471.1"
                     /translation="MLLKTSRRTFLKGLTLSGVAGSLGVWSFNARSSLSLPVAASLQG
                     TQFDLTIGETAVNITGSERQAKTINGGLPGPVLRWKEGDTITLKVKNRLNEQTSIHWH
                     GIILPANMDGVPGLSFMGIEPDDTYVYTFKVKQNGTYWYHSHSGLQEQEGVYGAIIID
                     AREPEPFAYDREHVVMLSDWTDENPHSLLKKLKKQSDYYNFNKPTVGSFFRDVNTRGL
                     SATIADRKMWAEMKMNPTDLADVSGYTYTYLMNGQAPLKNWTGLFRPGEKIRLRFING
                     SAMTYFDIRIPGLKMTVVAADGQYVNPVTVDEFRIAVAETYDVIVEPQGEAYTIFAQS
                     MDRTGYARGTLATREGLSAAVPPLDPRPLLTMEDMGMGGMGHDMARMDHSQMGGMDNS
                     GEMMSMDGADLPDSGTSSAPMDHSSMAGMDHSRMAGMPGMQSHPASETDNPLVDMQAM
                     SVSPKLNDPGIGLRNNGRKVLTYADLKSRFEDPDGREPGRTIELHLTGHMEKFAWSFN
                     GIKFSDAAPVLLKYGERLRITLINDTMMTHPIHLHGMWSDLEDENGNFMVRKHTIDVP
                     PGTKRSYRVTADALGRWAYHCHLLYHMEMGMFREVRVEE"
     gene            7391..7840
                     /locus_tag="QBF46_002296"
     CDS             7391..7840
                     /locus_tag="QBF46_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004388333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein"
                     /protein_id="HDQ6674472.1"
                     /translation="MNILITATAFTALFCGAAFAQSSDIAHEAHRFVNNASAVSHVNS
                     STHENLPDRVNKNNTPSFSEMNEHERAIVAHSFMNNSASYAHQKMIEEHKKMLSDSGA
                     NSKTSSSSFNELNAGEKAALVHEQVNNAGAEAHQTQARKLRGLYSSR"
     gene            8115..8852
                     /locus_tag="QBF46_002297"
     CDS             8115..8852
                     /locus_tag="QBF46_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDQ6674473.1"
                     /translation="MYSTDVVKENAYLSATRSGLESNEIAILQRSLPSRFNLRHLKKN
                     ESLKLVLQKKAGKSRVVAYKFTSGSFNYTAYRISDKKFYNLSDTSGKGSLDYPLPATA
                     RLSSPFNPARLNPVSGKVSPHNGIDYSMPMNTKIVSVIDGKITRAEYNSTMGYFVEVT
                     GKAGVKTRYLHLNKILVTKGARVTRGGAIALSGNSGRSSGPHLHYELVINNNPVNSLA
                     FRASAPADNKLEQHAFAHARDYERYLD"
     gene            complement(8886..9083)
                     /locus_tag="QBF46_002298"
     CDS             complement(8886..9083)
                     /locus_tag="QBF46_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HDQ6674474.1"
                     /translation="MKSTTYALIAVAAIAAFALLREHWSHVAGYWPYLLLLVCPLMHL
                     FHGHGGHGDHQHHGSENDKKN"
     gene            complement(9124..11571)
                     /gene="silP"
                     /locus_tag="QBF46_002299"
     CDS             complement(9124..11571)
                     /gene="silP"
                     /locus_tag="QBF46_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag(+)-translocating P-type ATPase SilP"
                     /protein_id="HDQ6674475.1"
                     /translation="MKNDNAVQHNNQTASEQTLSPDEGHVLHKVRDPVCGMAILPDRA
                     HSSIRYQDHQLYFCSASCESKFKAHPDRYLTEDASEHSHHHHHDHHEVSPDQIKQPHH
                     QAEKENSEGVWTCPMHPEIRRSGPGSCPVCGMALEPLVATASTGPSDELHDMTRRFWL
                     GLLLAFPVLVLEMGSHLFPDLRNTVPPQYNTWLQLLLASPVVLWCGWPFFARAGMSLR
                     NRSLNMFTLVAMGTGVAWVYSVIATVFPSWFPASFRNMDGLVAVYFEAAAVITVLVLL
                     GQVLELRAREQTSGAITALLNLAPKTARRLDHDGHETDINAEDVLPGDKLRIRPGESI
                     PVDGIVIEGKTTVDESMVTGESMPVTKTKGDPVIGGTINQTGSLIIRAEKVGDETMLS
                     RIVQMVADAQRSRAPIQRMADSVSGWFVPLVILIAVVAFVIWSVWGPEPRMAHGLIAA
                     VSVLIIACPCALGLATPMSIMVGVGKGAQAGVLIRNAEALERLEKVDTLVVDKTGTLT
                     EGSPTVTGIISLNPGGEASLLRVAAAVEKGSQHPLGMAVVRAAQHRGIMIPAVSDFNA
                     PSGKGVSGDVEGQRVVIGNELAMQENSIVIDNQKAVADTLRMEGATVIYVATDGYLAG
                     LIAISDPVKATTPDALKALRQAGIRIVMLTGDNQLTAEAVARKLGIDEVEAGVLPDGK
                     KAVITRLKASGHVVAMAGDGVNDAPALAAADVGIAMGTGTDVAIESAGVTLLKGDLMI
                     LNRARHLSEITMKNIRQNLFFAFIYNALGVPVAAGLLYPVYGILLSPVIAAAAMALSS
                     VSVIVNALRLKSVRLGK"
     gene            complement(11698..12138)
                     /locus_tag="QBF46_002300"
     CDS             complement(11698..12138)
                     /locus_tag="QBF46_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674476.1"
                     /translation="MKKVVLMALALGLSLPAMASEKVIDMYKSENCGCCSLWGKAMEK
                     DGFEVRTHVMNDQALSALKEKHAIPAGLRSCHTAVAGNLIIEGHVPATTIHKAMQSGS
                     GIYGLATPGMPAGSPGMEMGARKEAYDVIAFSPDGSKKVFQRIE"
     gene            complement(12225..15371)
                     /gene="silA"
                     /locus_tag="QBF46_002301"
     CDS             complement(12225..15371)
                     /gene="silA"
                     /locus_tag="QBF46_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit SilA"
                     /protein_id="HDQ6674477.1"
                     /translation="MIEWIIRRSVANRFLVMMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     LYWARSRVLEYLNQVQGKLPAGVSSEIGPDATGVGWIFEYALVDRSGKHDLSELRSLQ
                     DWFLKFELKTIPNVAEVASVGGVVKQYQIQVNPVKLSQYGISLPEVKQALESSNQEAG
                     GSSVEMAEAEYMVRASGYLQSIDDFNNIVLKTGENGVPVYLRDVARVQTGPEMRRGIA
                     ELNGQGEVAGGVVILRSGKNARDVITAVRDKLETLKASLPEGVEIVTTYDRSQLIDRA
                     IDNLSSKLLEEFIVVAIVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWDHQHPGEQIDNATRWKVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYSMAGAAALAIIVIPILMGFWIRGK
                     IPAETSNPLNRVLIKAYHPLLLRVLHWPKTTLLVAALSIFTVIWPLSQVGGEFLPKIN
                     EGDLLYMPSTLPGVSPAEAAALLQTTDKLIKSVPEVASVFGKTGKAETATDSAPLEMV
                     ETTIQLKPEDQWRPGMTIDKIIEELDRTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLSDIDVTAQSIEAVAKTVPGVVSALAERLEGGRYIDVDINREKASRYGMTV
                     GDVQLFVSSAIGGAMVGETVEGVARYPINIRYPQDYRNSPQALREMPILTPMKQQITL
                     GDVADIKVVSGPTMLKTENARPASWIYVDARGRDMVSVVNDIKTAISEKVKLRPGTSV
                     AFSGQFELLEHANKKLKLMVPMTVMIIFILLYLAFRRVDEALLILMSLPFALVGGIWF
                     LYWQGFHMSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAHPELSRKETFTPEGLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLIWLRKHKKSVS"
     gene            complement(15382..16674)
                     /gene="silB"
                     /locus_tag="QBF46_002302"
     CDS             complement(15382..16674)
                     /gene="silB"
                     /locus_tag="QBF46_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit SilB"
                     /protein_id="HDQ6674478.1"
                     /translation="MASLKIKYAAIIISSLIAGGLISVTAWQYLNSSQKTVPAEQKAP
                     EKKVLFWYDPMKPDTKFDKPGKSPFMDMDLVPKYADESGDKSSGGIRIDPTQVQNLGL
                     KTQKVTRGMLNYSQTIPANVSYNEYQFVIVQARSDGFVEKVYPLTIGDHVKKGTPLID
                     ITIPEWVEAQSEFLLLSGTGGTPTQIKGVLERLRLAGMPEEDIQRLRSTRTIQTRFTI
                     KAPIDGVITAFDLRTGMNISKDKVVAQIQGMDPVWISAAVPESLAYLLKDTSQFEISV
                     PAYPDKTFHVEKWNILPSVDQTTRTLQVRLQVTNKDEFLKPGMNAYLKLNTQSQEMLL
                     IPSQAVIDTGKEQRVITVDDEGKFVPKQIHVLHESQQQSGIGSGLNEGDTVVVSGLFL
                     IDSEANITGALERMRHPEKTENSMPAMSEQPVNMHSGH"
     gene            complement(16788..17141)
                     /locus_tag="QBF46_002303"
     CDS             complement(16788..17141)
                     /locus_tag="QBF46_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone SilF"
                     /protein_id="HDQ6674479.1"
                     /translation="MRNSLKAVLFGAFSVMFSAGLHAETHQHGDMNAASDASVQQVIK
                     GTGIVKDIDMNSKKITISHEAIPAVGWPAMTMRFTFVNADDAINALKTGNHVDFSFIQ
                     QGNISLLKSINVTQS"
     gene            complement(17169..18554)
                     /gene="silC"
                     /locus_tag="QBF46_002304"
     CDS             complement(17169..18554)
                     /gene="silC"
                     /locus_tag="QBF46_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel SilC"
                     /protein_id="HDQ6674480.1"
                     /translation="MFKLKLLSISTIFILAGCVSLAPEYQRPPAPVPQQFSLSKNSLT
                     PAVNSYQDTGWRNFFVDPQVSRLIGEALNNNRDLRMAALKVEEARAQFNVTDADRYPQ
                     LNASSGITYSGGLKGDKPTTQEYDAGLELSYELDFFGKLKNMSEADRQNYFASEEARR
                     AVHILLVSNVSQSYFSQQLAYEQLRIARETLKNYEQSYAFVEQQLVTGSTNVLALEQA
                     RGQIESTRAEIAKREGDLAQANNALQLVLGTYRAVPSEKGIKGGEIAPVKLPPNLSSQ
                     ILLQRPDIMEAEYQLKAADANIGAARAAFFPSITLTSGLSSSSTELSSLFTSGSGMWN
                     FIPKIEIPIFNAGRNKANLKLAEIRQQQSVVNYEQKIQSAFKDVSDTLALRDSLSQQL
                     ESQQRYLDSLQITLQRARGLYASGAVSYIEVLDAERSLFATQQTILDLTYSRQVNEIN
                     LFTALGGGWVE"
     gene            18744..19424
                     /gene="silR"
                     /locus_tag="QBF46_002305"
     CDS             18744..19424
                     /gene="silR"
                     /locus_tag="QBF46_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver response regulator transcription
                     factor SilR"
                     /protein_id="HDQ6674481.1"
                     /translation="MKILIVEDEIKTGEYLSKGLTEAGFVVDHADNGLTGYHLAMTAE
                     YDLVILDIMLPDVNGWDIIRMLRSAGKGMPVLLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGNTMITESQLKVADLSVDLVSRKVSRAGNRIVLTSKEFSLLE
                     FFIRHHGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRAKIDNDYGTKLIQTVRGVG
                     YMLEIPDA"
     gene            19417..20898
                     /gene="silS"
                     /locus_tag="QBF46_002306"
     CDS             19417..20898
                     /gene="silS"
                     /locus_tag="QBF46_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005763473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver sensor histidine kinase SilS"
                     /protein_id="HDQ6674482.1"
                     /translation="MHSKPSRRPFSLALRLTFFISLSTILAFIAFTWFMLHSVEKHFA
                     EQDVSDLQQISTTLSRILQSPADPDEKKVSKIKESIASYRNVALLLLNPRGEVLYSSA
                     QGAALRPAVNSADFSEHSRARDVFLWTVEDTARAMDTGSGMKMETYRIIASSGQATFQ
                     GKQQNYVMLTGLSINFHLHYLDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVS
                     NAIKNITSENLDARLEPTRVPIELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRT
                     PITNLVTQTEIALSQDRTQKELEDVLYSSLEEYNRMTKMVSDMLFLAQADNNQLIPDR
                     VRFDLRAEVMKVFEFFEAWAEERNIMLKFNGMPCLVEGDPQMFRRAINNLLSNALRYT
                     PEGQAITVSIREQESFFDLVIENPGKPIPEEHLSRLFDRFYRVDPSRQRKGEGSGIGL
                     AIVKSIVEAHHGRVQVESDVHSTRFILSVPRLEKMIPDTQCWE"
     gene            21143..21574
                     /gene="silE"
                     /locus_tag="QBF46_002307"
     CDS             21143..21574
                     /gene="silE"
                     /locus_tag="QBF46_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="silver-binding protein SilE"
                     /protein_id="HDQ6674483.1"
                     /translation="MKNIVLASLLGFGLISSAWATETVNIHERVNNAQAPAHQMQSAA
                     APVGIQGTAPRMAGMDQHEQAIIAHETMTNGSADAHQKMVESHQRMMGSQTVSPTGPS
                     KSLAAMNEHERAAVAHEFMNNGQSGPHQAMAEAHRRMLSAG"
     gene            21722..22072
                     /locus_tag="QBF46_002308"
     CDS             21722..22072
                     /locus_tag="QBF46_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDQ6674484.1"
                     /translation="MKARNTLFAVLMLSLPAISAEHSEMKMTDMSTSASSQEYMAGMK
                     DMHDKMMAAVNESDPDKAFAKGMVAHHEGAIAMAETELKYGKDPKMRKLAQDIIKAQK
                     GEIEQMNKWLDSQK"
     gene            complement(22241..24067)
                     /locus_tag="QBF46_002309"
     CDS             complement(22241..24067)
                     /locus_tag="QBF46_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6674485.1"
                     /translation="MRLRKLKLKNFRGYKNSTEIIIDESMTGIVGRNDFGKSTLLEAL
                     AIFFETEGMKADKNDMNCFSLREGDGRFEIACEFDDLPDFIMIDDRVQTTLASEHLLN
                     EDGNFEIVKTFKATTSGKPEQTCIRCIHPDEEPLRNLLGMKISELKAVGKEVEKNVAD
                     KRTASLWRQAIREAAAPYTCSEIMLDVDKEFGTDTKSLWGKILDLLPTYAIFKADRES
                     SDGDSEAKNPLQQAVKDAQAALQDKITALENEIQDSVLDVAQRTLDKLREMAPELASE
                     LTPRFKEKPKWTFNFTLDGENGIPINKRGSGIRRLILLNFFRAEAEKNVAGTPRNVIY
                     AIEEPETSQHPNYQMMLMKALLALAGQPHRQIIVTTHVPALAGLIPVEGVRYVTRNEA
                     GEPVVKMPDDAVLKEATESLGVLPETGMERAQGIVLVEGKSDVTFLRHAASSLKQSGA
                     LPASLEDVKIVPVLIGGCGSVKHWVTLNLAKDLGLPWCVFLDSDIGGDPAQVLSIQKR
                     KKEVEEAGKVFFATRKREIENYLCPDLIEEITGVAVTFTDTCDAKKIIGRAVGMKPDN
                     VLDKFWPQMTSERIISRSTYHDGTQERSELVEILSDIVSMTR"
     gene            complement(24631..24930)
                     /locus_tag="QBF46_002310"
     CDS             complement(24631..24930)
                     /locus_tag="QBF46_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6674486.1"
                     /translation="MNTQYALKTLNQLRPVLIGFRKANGLTQKDVSERLGITQQTYAR
                     LEANPASAGFERLFRVFSVLGVEIVLSSREPLSDAKPEYGAMHNYSSLPARREKW"
     gene            complement(25283..26209)
                     /locus_tag="QBF46_002311"
     CDS             complement(25283..26209)
                     /locus_tag="QBF46_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674487.1"
                     /translation="MVYENELWHSFLLRSQIMYNLSNCRNIISKHGALRYDAFPRFEL
                     IDMYKLHSLQDIYDILATRNGYILTSNIVSLSSGVYGYFNAVEDACRKNFHITNSYPL
                     VNISNIRYCHKCIVEDIHSKGIGYLRHRWLFESKCAVHSTSLYEVCFDNYLNAVKGLS
                     DLIISGINPHGYCSLVVDVSNPFKNPVYILPCARNKILKWISDNKNMLIYFLFDLFKC
                     KSHSSLSKVIEVKIMHDRYISAVFKMLSEVNFCNFNSFISDIFEDVSYASIGLDDYSV
                     NVHFLRLRAADCKFCQLNGKYFCSLYNVKLLR"
     gene            complement(26221..27777)
                     /locus_tag="QBF46_002312"
     CDS             complement(26221..27777)
                     /locus_tag="QBF46_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDQ6674488.1"
                     /translation="MRMLPVLPDESLFSRFCRTTTVYGMSPSSLLTIIFNKPDMNVHP
                     ILNSGLKAISLHTSESADQLWHEQTLLPLFAWALPISRNEIMDFNTTPARLNRLCRLS
                     NFSLGQRTLLKFCPVCAREDTFHYGVTYWHLAHQLHGVTTCHRHPVALESIHVPSSPH
                     IRIGLMPPVSYTEQLSNEIDFDFAKFCYESLNIIRRKDITHPNYMDVLKKLNLLSLDG
                     NLKKNVFYAHVYAKCQLFGEGSSGLIPTSLTDYHYWEPILKDKCCQHPTKHLLLCYCL
                     LNTCWPTYAGSRTNKKKEIFKSHKKYSFHIVENNTSVSNLGKEFSRSRCYIKTLIYKK
                     YLRAFKRNTKINIFTELLIKSMAVRGFSLASIAEKNSLSEGAVSSVISSCYGLCSWRK
                     KCKKDSLRRRHKQKILRFIHNQSVSITRKLVKESCYASFYWLNKHECDWLNSCLPKTI
                     RCYKNKRVDWSERDIISSSLINDVLSQGQYSMSLTSLDALLGGHGWLLKYRDKLPMTM
                     ILLRKMELIK"
     gene            complement(27818..29266)
                     /locus_tag="QBF46_002313"
     CDS             complement(27818..29266)
                     /locus_tag="QBF46_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ6674489.1"
                     /translation="MNLIRRVSAIYKEQELPEYRGNPLIEALPEALTEDEVLLEMSYF
                     PEIDEKIRWTAPANVREQYVERIKKFRCPQTNLIQAYKMILRALRESYAARNPLKSGT
                     IQYLHYYGNERPDIEPESGYFKSQAETITIVGMSGSGKTTMIEQVMDHFPQIIEHSSY
                     KGVFPGFSKQIVWVKINCPYNSSVRDLCEEILQKLDDAIGIERTTPEIRNGALARQIA
                     QRIKSSFLGILVIDEMQRLKFSRTGGESKLIDFLHEIVDSMGVSMVFCGNHPFDETLT
                     KKMRIARRAESGGYMKIKNVRYDSQDWQSFIHYLWPLQWTNVETPLNDELNEKLFILS
                     KGNIGLAQMIYRGAQLKVIGSGNEIITGAVLSASVPVLVSHTEEYKDTPLPGAATEDE
                     EAKWLSASNEGDASAKIMAEKSLKSLIPGDIDRPQHKEFVGKIDEVLRNFDEKILSLD
                     HDLVINRTAEKKNTYDALQRCGLINIDPLNKL"
     gene            complement(29266..31389)
                     /locus_tag="QBF46_002314"
     CDS             complement(29266..31389)
                     /locus_tag="QBF46_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6674490.1"
                     /translation="MDIIRNSVWLSQGTDLLAEGLYRVLDFDRKVDLLILFKIKSERT
                     GKPIPFSFSMFKYYIESNSITCKDYIYPSYMLVDEKELTDKDRGRRDENYNIIKDLVD
                     DRMFLFDYALHKKSHLLMDYSRNKKISQYTIRTLLALYWRHGQDIYALLPAFSNCGAA
                     GKSRIKHEIKLGNSKKNRALPNERSRVFILNERDINNIRKSLITYHYKVNGDTIKKTL
                     ERHIDLYFRDEIKTANLENRAPYVPSLKQFSYWNKKLFTKDFSINKKNTKKEIDLKMR
                     ALLGSVANTTVLPGDVFEIDSTVADVHLISSLNRRKVIGRPTIYTVVDRATRMIVGLH
                     VSLYHASWRAARQALANCFMPKKEYCRLFGISITDDDWPCSHIPLTLMCDNGEMIGLK
                     PQEEMTPLTKLEFAPVGRGDRKSIVERCFGILNDEVIHRLIGTTRRGKIVKGEPTPQS
                     RACLTIQEVTSLLIREILAHNQRTYEELAYINPLLIENDLVISPKNSWMISLKHGRFS
                     ARAVGADEVIARLLIPVNANITAGGIQYNNLFYECDPEIASGVRVFGRTTCEARIDDN
                     CVDYIYVRFDKNSIFKKHYLLKKRDVFKGKAHLDTDVMADWVDTQKEINLFTLDSLSN
                     INNKDEFNRKGNERLNEIYESRRVHGKDIKKNRKNELDSLGRTHVANGRSEPLLPSSS
                     NVLLLPGREEKQRWLKGKKKTEDAE"
     gene            complement(31376..32218)
                     /locus_tag="QBF46_002315"
     CDS             complement(31376..32218)
                     /locus_tag="QBF46_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 transposase TnsA N-terminal
                     domain-containing protein"
                     /protein_id="HDQ6674491.1"
                     /translation="MARGRRLKSYLDYENALGDGIGVGYGQSYQPWLRAQDVKSRGNR
                     SIVFGLKTFRNHHLLSSVESNFFYLAEFNDSVIDIREQFPLFPLRLTQQIANHLHFQH
                     PMVRGVRGVPVEVLNVMTTDFLLTLRTPEGGLRYKAIAVKHNESIPEREAQKLEIERM
                     FWQLIDVEFQIYVGSELNNVVGKNICWATSVLRDGSEFYDKYPLDKILWKLKPDVYPI
                     VGLRAMISSIFGVDAQEAMMLLQAMIGLKMINVDLSYPILETGLIKIISNDHYIGLNA
                     NGYY"
BASE COUNT         8525 a         7990 c         7131 g         8709 t
ORIGIN      
        1 tatcctttaa cctgaaaaag actttaaagt tgtaaagttt taaaaaaaac acatacaaaa
       61 catgatgcta agtgtgtatc acacgctgtt ccaacattat gccttgatgt tcttgcagga
      121 actgcaattt acaggaggaa aaaggtgttt ttgtagttat ctggcgttac gtgacagtgt
      181 tggtcagata cttccacctc ttcagagtta aagtttgata tatgggaaaa aaaaacgctc
      241 ccggacggga gcgtaagtca gtgacttcgg cgtcaaatga gggtctaaca agtggagctg
      301 cgggttaatt ctgggtgatc tgactgcgat gcttatcagc ctgctgctgg tgaacaacgg
      361 aagactgacc attattcgcc ttctcatgta cagctgcggc cttctcatgc tcgctcattt
      421 cagaaaatga ctttgctgtt tcgttggtag ctccgggttt agctttcatc atttttttat
      481 gcatttcagc catgtcctgg tgggcagcag aattgctgtt ttgcatcatg tcatgggaca
      541 ttgcggcctg atcgtgactg ctcatatcca tcattgtcat gctgtctccc tgaattgcac
      601 ttttttcagc agatgactgc atctggtggg caggtgcctg tgcattattt acctggtcat
      661 gcatgttcat tgtctctgca gccatggcag atgaagcggc agccataatg gcaacaaatg
      721 atacgagaat ctttttcatg gttgggtctc cggtgtttac atacccaaac aatcaatggc
      781 ttataacaga gaaagcagtt gatctcaggg cgggttaatc atcactccag gaaccaggtg
      841 attgtgttat cacgctgctc ctgatttatg aataattata aaaaaccgcc tctgtttatc
      901 gcgtgactga atgatgacaa ttttgtcacc ttccaggttt ctcctgaaca acgggattaa
      961 tccattaaca ggcgcacttt gaacacaatt tcacgcccct gctgttctgc tgacagctcg
     1021 ccgccgtgag catgaatgat cgaccttgta attgataatc ccagccccgc gccttccgtg
     1081 ttgtagaacc ttgatgagtc tgcgcgatag aaccggtcaa acaaacgttc cagattagcg
     1141 ggaacctggc cggacatcgt attcgtaatc atcacgttca cacagtcact gtcacgctca
     1201 aggtgtatcg ctgtacaggt gttatcggga gaatacttga ttgcattgga aagcaggtta
     1261 ctgaaagcac gtcggagcat atcgctgtct ccggcaacaa cgccctctcc ttcaaccgtg
     1321 attgtctttc ctgtttcgtc tgccaggggc tcgaacaact cacgtaattc attcagttcg
     1381 gctgccagat ccacatcatg tttatccagc cgcagcagac catgctctga acgtgccaga
     1441 aaaagcatgt cactggtcat tcgtgacaac cttttcagtt cttccaggtt agcgaataaa
     1501 atttcgcggt aatgcgaaac atccctttcc ttagccagtg caaactgcgt ctgcatcatc
     1561 agattactga ctggtgtgcg cagctcatgc gcgatgtcag acgagaaatc tgacagtttc
     1621 cggaatgccc cctccaggcg atcaaacata ttattgaact cctgcatggt ctcagagatt
     1681 tccggcggag ccagatcggg atttagacgc tgatccaggc tgtgtacggt catggaggaa
     1741 gccagactgg tcatttcccg tagcggtttc agaccaatac gtgtggtcag ccagcccaga
     1801 aaaacagaaa taaagaccag accgatattg aaccagaaca gccaggtact gagtttgtcc
     1861 ataaacaggg tgtgataccc agtatccgtg gcaaccgtaa tgatgacatg tttgctttta
     1921 ccctgttccg gcgtcacggc aacccgccgc gagatactgc ggtacacggt gttattttct
     1981 tccgtctgga tcatatagtc gagaatatca cccgacttat taagcaggac cgctggaaca
     2041 acagaatttt tggcatagag ttcaacaatt ttttcatttt ccatgttttt tatagaaatg
     2101 aataagccat tgtgccccac catcgcatcg tttatttttt ctgataatga cttaatatcc
     2161 gttttgttcc tgaacgtctc tgttttaaga aactcttcgg tgagctgaag tttacctgtc
     2221 agaaaatcgc ggtcctgatt atcgaaatag ccattaaggg tgctaatcag gataaaactt
     2281 gataaccacc ataccgtaag catcaccgca gaaaaaatca ggctcaggcg tgtggtcagg
     2341 gaaattttga acctcactct tctctgatct ccaggacata tccggcaccg cgaacggtat
     2401 ggatcagttt tggctcaaag tcatcatcaa ttttacttct cagacgtctc acggcgacat
     2461 caatcacatt cgtatcactg tcaaaattca tgttccagac cagggacgag ataagactcc
     2521 tgggtaacac ttctccggtg cgttgcagca gcaactcaag cagaacgtat tctttaccgg
     2581 tgagatggat cttcttcccc gaacggatca cggtccggcg caccatatca acggtcatat
     2641 cggcgatggt gcagactgtt gcggcctgcg agcgtgcccg gcgcagtagg gttcttacac
     2701 gtgcaaccag ctccgtaaaa tcaaagggct taatcaggta gtcatctgcg ccaagctcca
     2761 gtcctttcac tttgtcccgc acgttgtcct ttgcggttaa aaacaggacc ggttcttcgt
     2821 gcccggactc cctcagtgcg ctgatgattt gccacccgtc gaggaaaggc agcatcacgt
     2881 ccagtattat caaatcatac tgtcccttcg acgcggcccc gagaccatcg cggccattat
     2941 taaagagatc ggcctgatag ccttcctcaa ccagtccctg ctgcaggtaa cgacctgttt
     3001 tttgttcgtc ttcaacgatt aaaatacgct gcatggtcaa ctcgctgata tgaaagtaaa
     3061 aatctcacgc atgagctttg gctgtcccct agctgacctg agacggagca agcattccga
     3121 gccacgctac ggcgcccaga atgataatcg caacaacgaa ttctgtcagg atgctgtttc
     3181 gcatcagggc aacgctgcga tcataattcc cttccctgac cataacttca agccggggac
     3241 ccaggtgaaa ccggtttgct gcagccagaa gaagcatcag aacaaacaga gccgtcttgg
     3301 caagcaatat cctcccccag gaactgttga ataagggagt taagttaccc tcagcaatat
     3361 acagatagtt gaccagcgca ctcaggatca gggctacaac aatcaccgtt cctgccgtgg
     3421 caaattttgc cagggagtca gatatcacaa tgacgctctg tgcattatgc tcgtttctgc
     3481 gcatcagcag gatagcaaat gcaaccagag cacctgtcca ggcacctgca gcgccgagat
     3541 gggtcagatc gctcagtaaa tggagatagt aatgcagacc gtcatgcata acggcgtgtc
     3601 ctccccaggc aagtgtagcc agcgccacgc ccccactcat cgtcatcagc aggcaggaca
     3661 atactctctt attagtgtaa aggaacaaag caccgagtgt ggtaaacagg gcacagagcc
     3721 tgacaatcca gctaataccc acatcagttt cttctatcac catctcgata acatggatgg
     3781 ataattctct gaggtcagtt actccactca tggcattaga taccaggagc atattaatgc
     3841 cagtaagaat gatgcctgta acaacagcaa aggttataaa cgacctgaaa ttagtcaggt
     3901 tataggtttc atgtctgaca ccgcttattc catatatctg aaaaaatggc aatccaaata
     3961 ttaccatcaa atccagataa agaagaaaac gaataacaat cataatcagg tcgttcataa
     4021 tattacttca ctgtaaaggt gtaattaccg gtaatagggt gcgtatctga agaaaccgcg
     4081 cgccagtcaa cacgataagt gccagcgggt aaaggctctc gcggaataat gaccatcgat
     4141 ttagggtcag cgcctggcgc cacttttgcc gcgaccggca tcggagaatg tgatgacatg
     4201 cctttcatac ccgtcatcgt taattttgca cctgagaatt tcacggtcag attttccgag
     4261 aaattaagct gaatcttttc cggggccgct acggctgaat cagcctgtgg cacagagctt
     4321 tttaattccg gatgggccat agcagagaaa gcaacgccca taacgaggcc acctgtaaga
     4381 atggctttat ttaaaatcga cattttattt acctgtttag ttgagtgttt tatatcagtg
     4441 cgttaaaacc agattctggc tcccgccagg aatactacct gatggtcttt ctcaccttct
     4501 cttttcgcca tatcggatgt tttcccgtaa agttgattcc aggaaacgcc tatatagggt
     4561 gcaaactcac ggcgtatttc atagcgcagc cggagcccca gctctgtgtc agtcagtccc
     4621 ctgccgcgac cccgcgattc atcatcctga ctgtagaaat tcacctcata ggatggctgg
     4681 agtatgagcc ggttagtcag taaaacgtcg tattctcctc ccagacgaag ggctgctttt
     4741 ccgccattac tgacaaaacc cgtaatttca gactcaaaat tatagagtgc cagcccctga
     4801 aaaccgacag cagcccaggt ccgggcagaa gcaggtctga aatcctgcct gacacccgta
     4861 accaaatccc accatgggcc aaccgcatgt ccccagagta actgcgcttc agccgcctcc
     4921 gtttccccat tgcttcgttc accttcactc tttagccaaa tccgatctgt gtcgcctcca
     4981 atccagctgt taacactcca gctgaaattg ttggtgttat ccgaccgttg ccattccagt
     5041 tgatccagca gaaccagata attaatcgca ctgtcgtgaa tcgcatgccc ctgtaaattg
     5101 ccgaatgcag ccttccggtc ggcatcggta acaggcggaa ttggcgttct gctctcagtt
     5161 acaatgggct ccattgacgt catctcagtg aaattctcat ctgctggcat ctgcatggca
     5221 gacatgtcgt gcccggcgtg gggatctgca gagacggagc ccgccgcaat agaaagctgt
     5281 gaggtaaaca aaccggcgac cagaacaggt atggccttca aatttctctt cattcgcatc
     5341 attcctccac ccggacttca cgaaacattc ccatttccat gtgatagagc aaatggcagt
     5401 gatacgccca gcggccaagc gcatctgctg tcactctgta actgcgtttt gtaccagggg
     5461 gaacatctat tgtgtgttta cgaaccatga aattaccgtt ttcatcttcc agatcgctcc
     5521 acataccatg caggtgaatg gggtgagtca tcatggtatc gttgatcagc gtgatcctga
     5581 gccgctcacc gtatttcagc agcaccggtg cggcatctga aaacttgatt ccgttaaatg
     5641 accaggcaaa cttttccatg tggccggtta aatgcagttc tatggtacgg ccaggttcac
     5701 gtccgtcagg atcctcaaag cggcttttca aatccgcgta cgtgagaacc tttcttccgt
     5761 tatttcgaag accaataccc ggatcattta atttcggaga gacgctcatc gcctgcatat
     5821 caaccagtgg gttatccgtt tctgacgcag gatgactttg catacccggc attccggcca
     5881 tccgggaatg atccataccg gccatgctgc tgtgatccat gggcgcggag gatgtcccgc
     5941 tatccggaag gtcagcaccg tccatagaca tcatctctcc gctgttatcc atgcctccca
     6001 tctggctgtg gtccattctt gccatatcat gtcccattcc ccccataccc atatcttcca
     6061 tggtcaacag aggacgggga tcgagggggg gaacggcagc acttaacccc tctctcgtgg
     6121 ccagtgtccc tcgagcgtaa ccggtcctgt ccatggattg tgcgaagatg gtataggcct
     6181 caccctgagg ctccacaatg acatcatagg tttcggcaac ggcaatcctg aattcgtcaa
     6241 cggtaaccgg gtttacatac tggccatctg cagccacgac cgtcattttc agcccgggga
     6301 tacggatatc gaaataggtc attgccgagc cgttgataaa ccgtaagcgt atcttttcac
     6361 cgggacggaa cagtccggtc cagtttttca gcggggcctg cccgttcatg agataggtgt
     6421 aggtgtagcc actgacatcc gcgaggtcag tcggattcat tttcatttca gcccacattt
     6481 tccgatcggc aatggtggct gacagccccc tggtattcac gtcgcggaaa aaagagccaa
     6541 cggttggttt attgaaattg tagtaatccg actgtttttt taattttttc agcaggctgt
     6601 gaggattttc atcggtccag tcagacaaca tgaccacatg ctcacgatcg taagcaaacg
     6661 gttctggctc cctggcatcg atgataatgg caccgtatac cccctcctgt tcctgcagac
     6721 cggaatggct gtggtaccag taagtcccgt tctgcttaac cttaaaggtg taaacgtagg
     6781 tatcatcagg ctctatgccc ataaaactca gccccggaac accatccata ttggccggaa
     6841 gaataatgcc gtgccagtga atggacgtct gttcattaag acggtttttg accttcaggg
     6901 taatggtgtc accttctttc cagcgaagaa cgggccccgg caggcctcca ttgattgttt
     6961 tggcctgacg ctcactgccc gtgatattga cggccgtttc accaatggtc aggtcaaact
     7021 gagtaccctg cagggatgcg gcaactggca ggctcagact ggaacgcgca ttgaaactcc
     7081 atacgccaag acttccggct acgccagaga gggttaaccc cttcaggaaa gttcgtcgag
     7141 acgttttcaa cagcatgcgc attcccttat ttaaagtatg gttactgaca gaattcgaga
     7201 accgatttaa attaaattac tggttcatcc acttacaatg aaatgaatct agcacacctt
     7261 aagaaacaaa ttcattacaa tcgtgtaatg tacatagctg tattacattt acgtcatctt
     7321 ccccacaggt tgattatttt tatatatgat cataaggaca tcttttatgt acctcagaag
     7381 gtaattacac atgaatatat taatcacggc cactgcgttt acagctttat tttgtggggc
     7441 agcttttgct cagtccagtg atattgccca tgaagcacat cgatttgtta ataatgcctc
     7501 agccgtcagt catgtgaatt cctcgacgca tgaaaactta ccggacaggg ttaataaaaa
     7561 caacacgccc tcattctctg aaatgaatga acatgaaagg gccattgttg ctcattcatt
     7621 tatgaacaac agcgcgtcct atgcacatca gaaaatgatt gaggaacata aaaaaatgct
     7681 gtccgacagt ggtgcaaatt caaagacctc ctcttcttct tttaacgaac tgaatgccgg
     7741 agagaaagcc gccctcgtgc atgagcaggt caataatgcc ggtgcggaag ctcatcagac
     7801 acaggcaaga aagcttcgcg gcctgtattc ctccaggtaa ctgcaggcgg gttagtgctt
     7861 agcagggctt tactgacggg ttacgtagca ggacatgagc cccatattgc tctgccgtaa
     7921 cctgtttcag tcccgttaat catcaccgtc aggctggtca tacagttccc agatcttcag
     7981 gagcaaacgg gaaacaagat atgttacaaa aatgacgacc atcaacggtg ctcccgatta
     8041 actggcagat gacacgcctc ctgaaaaccc ctagcatacg ccgcagtgtt catgctaacg
     8101 gcgaattcca gtaaatgtat tctaccgatg tcgtaaaaga aaatgcttac ctttcagcca
     8161 cccgctcggg gctggaatcg aacgagatcg ctatccttca gcgctctttg ccttcccgct
     8221 ttaatcttcg gcatttgaaa aaaaatgaat cattaaaact cgtactgcaa aagaaagcgg
     8281 gaaaatcacg tgtagtggct tataaattta cgtccggctc atttaattac acggcgtatc
     8341 gtatatcaga caaaaagttc tataatcttt ccgatacttc cgggaaaggc agtctcgatt
     8401 atccgttacc agccacagca agactcagtt cacctttcaa tcctgcaaga cttaacccgg
     8461 tatcgggaaa agtgagtccc cataatggca ttgattattc catgcccatg aacacgaaaa
     8521 tagtcagcgt catcgacgga aaaatcaccc gggccgaata caacagtacc atgggatatt
     8581 ttgttgaagt aacgggaaaa gccggtgtta aaactcgcta tctccacctc aataaaatac
     8641 tcgttactaa aggggccaga gttacccggg gaggtgctat tgcgttatcc ggtaacagcg
     8701 gacgttcatc cggtcctcat ttacattacg agctggtcat caataacaat cctgttaatt
     8761 cactggcgtt ccgggcatcg gcacccgctg ataacaaact cgaacagcat gcctttgcgc
     8821 atgccagaga ctacgaacga tacctggact gataacggga ccgcgacgcg gccccgtttg
     8881 ccggattaat tttttttatc gttttcactt ccgtgatgtt ggtgatcccc atgccctccg
     8941 tggccgtgga aaagatgcat tagcgggcag accagcaata acagatatgg ccagtaacct
     9001 gccacatgtg accagtgttc tcgcaggagg gcaaatgccg cgatcgcggc gacagcaata
     9061 agcgcatagg tggtactttt catactggac tccttctgtt cgtaactgac ccttcactca
     9121 gtgttatttc ccgagcctga cacttttcag acgcaacgca ttcacaatga cgctgacgga
     9181 ggaaagagcc atggccgccg ccgcaataac tggcgacagc agtattccat acacaggata
     9241 aagcagacct gcagccacag gcacgccaag tgcgttgtag ataaatgcaa aaaacagatt
     9301 ctgtcggata tttttcatgg tgatctctga cagatgacgg gccctgttca gtatcatcag
     9361 gtcgcctttg agaagggtga ctccggcact ttcaattgcc acatctgtac ccgttcccat
     9421 agctatgccc acgtcagccg ctgccagcgc cggggcatca tttacaccgt cccccgccat
     9481 cgcaaccaca tgaccagacg ctttcagtcg ggttatcact gcttttttgc cgtcaggcag
     9541 aaccccggct tcaacctcat ctattcccag tttccgtgcg actgcttcag cagtaagctg
     9601 gttatccccg gtgagcataa caatgcggat ccccgcctga cgcaaagctt taagcgcatc
     9661 cggcgtggtt gctttcacgg gatccgagat agctatcagg cctgcaaggt acccatctgt
     9721 ggccacatag ataacggtag cgccttccat ccgcaacgta tccgcaacgg ccttttgatt
     9781 atcaataacg atactgtttt cctgcatagc cagttcatta ccaataacaa cccgttgacc
     9841 ttcaacatcg cctgaaacac ctttccccga cggagcattg aaatcactga ctgcgggtat
     9901 catgatcccc ctgtgctggg ctgcccggac tacagccata cccagcggat gctgcgagcc
     9961 tttttccact gcggccgcta cacgtaaaag agatgcttcc ccacccggat tgagactgat
    10021 aatccctgtc accgtaggcg aaccttccgt gagcgtgcct gttttgtcga caaccagcgt
    10081 gtccactttt tcaagacgtt caagggcttc ggcattcctg attaacaccc cggcctgggc
    10141 tcctttgcct acccccacca ttatcgacat cggtgtggcc agccccagtg cgcaggggca
    10201 ggcaataatc aggaccgaca cagccgcaat cagaccgtgc gccatcctgg gctcgggccc
    10261 ccagacagac cagatcacga aagcaacaac cgcgataagt atcaccagag gaacaaacca
    10321 gcctgaaacg ctgtcagcca ttctctggat gggggcacgc gaacgctgtg catcagcgac
    10381 catctgaaca attcgtgaga gcatcgtttc atcaccgact ttctctgcac ggatgataag
    10441 actacctgtc tgattaatcg tccccccaat gacagggtca cccttcgttt tggtaaccgg
    10501 catagactcc ccggtcacca tcgattcatc aacggttgtt ttgccttcga tcacgatacc
    10561 gtcgaccgga atactctctc caggtctgat gcggagctta tcgccaggca ggacatcttc
    10621 cgcattaata tccgtttcat gaccgtcatg atccagccgt ctggcggttt tgggggcaag
    10681 gttcagaagc gcagtaatgg cgcctgaggt ttgttcccgt gcccgcagct caagaacctg
    10741 tcccagcaga acaagcaccg taataactgc tgcggcttca aaataaacgg ccaccaggcc
    10801 atccatgttt ctgaacgatg caggaaacca ggaggggaag acggttgcaa tgacgctgta
    10861 aacccaggct acgccggtcc ccattgcaac aagggtaaac atattcaggg agcggttacg
    10921 taacgacatt ccggcccggg cgaagaatgg ccagccacac cacaacacga caggggaggc
    10981 cagaagcaac tgcagccatg tgttgtactg tggcggtact gtattcctca agtcgggaaa
    11041 cagatgagat cccatttcga gtaccagaac cggaaacgcc agcaacaacc ccagccagaa
    11101 gcgtcttgtc atgtcgtgaa gttcatcact cggccccgtg gatgccgtag ctacgagcgg
    11161 ctccagtgcc attccacaga caggacagct tccgggacca ctgcggcgta tctccgggtg
    11221 catcggacat gtccacacac cttcagaatt ctctttttcc gcctggtggt gaggctgttt
    11281 tatctgatca gggctgactt cgtgatgatc gtggtgatgg tgatgggaat gttcactggc
    11341 atcttcggta agataacgat cgggatgggc tttaaattta ctctcacagc tggcggagca
    11401 gaaataaagt tgatggtcct ggtatcgaat gctgctgtgc gccctgtcgg gcaggatggc
    11461 catcccgcac acgggatctc tcaccttatg caatacgtgg ccctcgtccg gggataatgt
    11521 ctgctcagaa gcagtctggt tgttgtgttg cactgcattg tcatttttca cagtaactct
    11581 ccttatgcat accgaagcat cttctgtctt cagggtatgt catggatgcg attaccacga
    11641 atatcgccgg cacaggagtg cctgctaccg gcgtcccgtt atcagccgtt ccgctgacta
    11701 ttcgattcgc tggaagactt ttttacttcc atccggtgag aatgcgataa catcgtaagc
    11761 ctcttttcgg gctcccatct ccattccagg acttcctgct ggcataccgg gggtggcgag
    11821 accgtatata cccgaaccag actgcattgc cttatgtatc gttgtcgcag gcacatggcc
    11881 ttcaatgatc aaattaccgg caaccgcggt atgacaactt cgtagtcctg caggaatagc
    11941 atgcttttct ttcagggctg acagcgcctg atcattcatg acgtgagttc gtacttcaaa
    12001 cccgtctttt tccatcgctt tgccccacag ggaacaacag ccacagtttt cagatttgta
    12061 catatcaatc actttttcac tcgccatcgc gggtagtgaa aggccgagag ccagcgccat
    12121 tagaaccact tttttcatac tcacctctgt atattatcga tataaaccct gacgtcaggg
    12181 tgaatcagta aagaaggaca cccacagggg gtgccctttc aggttcagga cacgcttttt
    12241 ttatgtttgc gcagccagat taatttgtag gcggcaggaa taatgaacag ggacagcagc
    12301 ggagccgtga tcatcccacc aatcatgggt gccgcgatac ggctcatgac ttctgaacct
    12361 gcaccggttc cccagagtat tggcagcaga cccgcaatga tcaccgccac ggtcatggct
    12421 ttcggccgga cacgcagtac ggcaccatga tagagggctt catcaaggcc ttccggtgtg
    12481 aacgtctctt tacgggacaa ttccgggtgc gcttcaatgg catgacgcag atacatcagc
    12541 atgaccacgc caaactctgc tgccaccccg gccagggcga taaacccggt tccggtcgcc
    12601 actgacatat ggaagccctg ccagtacagg aaccatatcc cgccaaccag ggcaaacggc
    12661 aggctcatca ggatcaacag ggcttcatca accctgcgga atgccagata taacaggatg
    12721 aagatgatca tcaccgtcat cggtaccatc agttttaatt tcttattggc gtgctcgagt
    12781 aattcaaact gtcctgagaa tgccacactg gtccccggtc tcagtttcac tttctcactg
    12841 atggccgtct taatatcgtt caccaccgac accatgtccc tgccgcgggc atcaacataa
    12901 atccagctgg ctggccgggc attttcggtt ttcagcatgg ttggtccgga aacgacctta
    12961 atatcggcaa catcgcccag cgtaatttgc tgcttcattg gggtcaggat tggcatctct
    13021 ctcaacgcct gcggactgtt acggtaatcc tgcggatagc gaatgttaat agggtaccgg
    13081 gccacgcctt caaccgtctc acccaccata gccccaccga ttgctgaaga gacgaacagc
    13141 tggacgtcgc ccacagtcat cccgtaccgg gaagcttttt cccggttaat gtccacatca
    13201 atataacgtc caccttccag tcgttcagcc agtgcggaaa ccacaccagg tacggtttta
    13261 gcgactgctt cgatactctg tgctgttaca tcgatatccg acagaacagt acccgatacc
    13321 ttaataccta tcggactttt aatgccggtt gagagcatat caatacggtt acggataggc
    13381 ggtacccaga ggttggccag acccggtaaa cggacggtcc tgtcgagttc ttcaataatc
    13441 ttgtcaatcg tcatgcctgg acgccactga tcctcaggtt tgagctggat cgtggtttcc
    13501 accatttcga gcggcgcgga atccgttgcg gtctctgctt taccggtctt gccaaataca
    13561 gaagccactt caggaacgct tttgattaac ttgtctgttg tctgcaggag cgctgcagct
    13621 tctgccggag agacgccagg caaggtcgac ggcatataca gcagatcgcc ctcgttaatc
    13681 ttcggcagaa attcaccgcc cacctgactc agtggccaga taaccgtgaa aatggacaag
    13741 gccgcaacca gcagggttgt ttttggccag tggaggaccc gcagcagcaa aggatgatac
    13801 gctttgatca gcacccggtt cagggggtta ctggtctctg caggaatttt cccccggatc
    13861 cagaatccca tcagaatagg aatgacgatg atggccagtg cggccgctcc cgccatggag
    13921 tacgttttcg tgaatgccag cgggccaaac agacgacctt cctgcccttc cagggtaaag
    13981 ataggaataa aggacagggt gatgatcagc aggctaatga acaacgcggg tcccacttcc
    14041 acggaggcgt cggtaatcac cttccagcgg gtggcgttgt caatctgctc acccggatgc
    14101 tgatgatccc actcctcaag ccgtttgtgc gcattttcaa tcatcacaat ggcggcatcc
    14161 accatcgcac cgacggcaat cgctatccct cccagcgaca tgatattggc gttcagtccc
    14221 tggaagtgca tgacgataaa ggcgatacac aggccaagcg gcagagagat aatcgccacc
    14281 agggcagaac gtacgtgcca caggaacaga gcacagacga tggccaccac gataaactct
    14341 tccagaagtt tggaactgag gttatcaatc gcccggtcga ttaactggct gcgatcgtag
    14401 gtggtcacga tttcaacgcc ttccggcagg ctggccttca gcgtctccag tttatccctc
    14461 actgccgtga taacgtcgcg cgcattttta cccgaccgca ggatcaccac gccgccagcg
    14521 acttctccct ggccgttcag ctcggcaata ccacgcctca tttcgggccc ggtctgcacg
    14581 cgggcaacat cccgcagata aaccggcacg ccgttctcac ctgttttcag gacgatgtta
    14641 ttaaaatcat caatgctctg aagataaccg ctggcacgga ccatatactc cgcttcggcc
    14701 atttcaacgg atgaaccacc ggcctcctgg ttagacgatt caagagcctg tttcacttcg
    14761 ggcaggctga tcccatactg ggacagtttt accggattga cctgaatctg gtactgcttc
    14821 accacgccac caaccgaggc tacctcagcc acgttcggga tggtcttcag ttcaaatttc
    14881 agaaaccagt cctgcaaaga acgcagttct gaaaggtcgt gttttccgct gcgatcgacc
    14941 agggcatatt caaatatcca gcccactccc gtggcgtccg gaccgatttc agagctcaca
    15001 cccgcaggca gtttgccctg aacctgattc aggtattcca gcacgcgcga acgggcccag
    15061 tacagatcgg tcccgtcttc aaaaatgaca tacacatacg aatcaccgaa ctgtgaaaag
    15121 ccacgcacgg tttttgcgcc aggtacggac agcatggtgg tggtaagcgg ataggtgacc
    15181 tggttttcta caatctgcgg agcctgtccg ggatagctgg ttttaataat gacctgcaca
    15241 tctgacaggt caggcagcgc atcgaccggc gtgttaatta tcgtccatgt gccccagatg
    15301 ctgagaaaca gtgcgcccat catgaccagg aaacggttgg cgacagagcg ccggataatc
    15361 cattcaatca tcgtcgtctc ctcagtgccc tgaatgcata tttacaggct gctcagacat
    15421 tgctggcata ctgttttctg ttttttcagg gtggcgcata cgttccagcg cgcccgtaat
    15481 attggcttcg gagtcaatga ggaacaggcc actgaccacc acggtatcgc cttcattaag
    15541 accggagcca atgccggact gttgctgtga ttcatgcaga acgtggatct gtttcggcac
    15601 aaacttgcct tcatcatcaa cagtaatcac gcgctgttct ttgccggtat cgataacggc
    15661 ctggcttggt atcagcagca tctcctgact ttgggtattc agtttgagat aggcattcat
    15721 gcccggcttg agaaactcat ccttgttagt aacctggagg cggacctgaa gcgtgcgggt
    15781 tgtctgatcc acgctgggaa gaatgttcca tttttcgaca tggaatgttt tatccggata
    15841 agccggtacc gaaatttcaa actgcgacgt atctttcagc agataggcga gagattctgg
    15901 cactgcagcg ctgatccaga ccgggtccat cccctgaatc tgagccacca ccttatcttt
    15961 ggaaatattc atgccggtgc gcaggtcaaa tgcagtaatg acaccatcaa taggtgcttt
    16021 aatggtaaaa cgggtctgga ttgtgcgggt tgaacgcagc ctttgaatat cctcttccgg
    16081 cataccagcc agacgaagtc gctccagaac cccttttatc tgggttggcg taccgcctgt
    16141 accggataac agcagaaact cactttgtgc ctcaacccat tcaggaatgg tgatatcgat
    16201 aagcggagtg cctttcttca catgatcgcc aatcgtcagg ggatacactt tttcgacgaa
    16261 accgtcagaa cgcgcctgca caatgacaaa ctgatattcg ttgtaactga cattagccgg
    16321 gattgtctga gaataattca gcattcctcg cgtgactttt tgcgttttta atcccagatt
    16381 ctgaacctga gttggatcga tacggatccc gccactgctt ttatcgccac tttcatcagc
    16441 atatttcggc acaaggtcca tatccataaa aggcgatttt ccgggtttat caaatttggt
    16501 atccggtttc atagggtcat accagaaaag tacctttttc tccggtgcct tttgttctgc
    16561 tggtactgtt ttttgtgatg agtttaaata ctgccaggca gtaaccgata tcagcccccc
    16621 tgctatgagg ctgctgataa ttattgcagc atattttatc tttaaagaag ccatacaatt
    16681 tctcgctgaa aaatcaaaac acctggcata tgcgcacgat tattcattca cagtaatccc
    16741 ataatgaatg ttcaggcgca ctggatgtat tcgctccgga ctgttaatca ggattgcgta
    16801 acgttaatgc ttttgagtaa ggagatattg ccctgctgaa taaacgagaa atcgacatgg
    16861 ttgccggttt tcagggcatt gatagcgtcg tctgcattaa caaaagtgaa gcgcatggtc
    16921 attgcaggcc agcccaccgc cgggattgct tcgtgcgaaa tggtaatctt tttactattc
    16981 atatcaatgt ctttaacgat accggtgccc ttgataacct gctgtaccga agcatcactg
    17041 gcagcattca tatcgccatg ctgatgtgtt tcagcatgaa gaccggcaga aaacatgaca
    17101 gagaaggcac caaataaaac ggctttaagt gaattacgca ttttaatttc ctgattaatt
    17161 gaataaattt actctaccca accgccaccc agcgcggtaa acagattaat ttcgttaacc
    17221 tgtcgggaat aggtaagatc gagaatggtt tgctgcgtcg cgaagaggga acgttctgca
    17281 tccagcactt cgatgtaact gacagcacca cttgcataca atcctctggc acgctgtaga
    17341 gttatctgaa gtgaatcaag ataacgctgc tgtgactcaa gttgctggct aaggctgtcg
    17401 cgcagcgcaa gcgtgtcgga aacatcctta aaggctgact gaattttttg ttcgtaatta
    17461 accacggact gttgctggcg aatttcagcc agcttcagat tggctttgtt cctgccagca
    17521 ttaaatatag gaatttcaat tttaggaata aaattccaca ttccacttcc tgacgtaaag
    17581 aggctggaca gctccgtact gcttgaggag agaccactgg tcagggtaat ggacgggaaa
    17641 aaagccgctc gcgctgcgcc aatattggca tcagccgctt tcaactggta ttccgcttcc
    17701 ataatatccg gacgctgcag caatatttgt gacgacagat ttggtggcaa ttttactggt
    17761 gcgatctccc cacctttaat ccctttttct gacggaactg cgcggtacgt tcccagcacc
    17821 agttgcaggg cattgtttgc ctgagccaga tcgccttctc gtttggctat ttcggcgcgg
    17881 gtactttcga tttgccccct ggcttgttca agcgccagaa cgttcgtact cccggtcacc
    17941 agttgttgct caacgaatgc ataagactgt tcataatttt tcagcgtttc ccgcgcaata
    18001 cggagttgtt cgtacgccag ttgctggctg aaatagctct gtgaaacgtt ggagaccagc
    18061 aggatgtgta cggcacgacg ggcttcttcg ctggcaaagt agttctggcg atcagcctca
    18121 ctcatgttct taagtttgcc aaaaaaatcg agctcatagc tgagctccag acccgcgtcg
    18181 tactcctgcg tggtcggctt gtcacctttc agaccaccgc tgtatgtaat cccggatgag
    18241 gcattcagtt ggggataacg atctgcatcc gtgacgttga actgggcccg ggcctcttca
    18301 accttcaggg cagccattct caaatcacgg ttattattca gggcttcacc gatcagcctg
    18361 ctgacctggg gatcgacaaa aaagtttcgc cagcccgtat cctgatagct gtttaccgca
    18421 ggcgtcagac tgtttttaga caatgaaaac tgctggggaa ccggagctgg cggacgctga
    18481 tattcaggtg ccagagaaac acaaccagcc aggatgaata tggtactgat gctgagtaat
    18541 tttaatttga acataacgct tctcgtacag gcagacctgg taaatggttc aaacgaagtc
    18601 cagagtattg atagggatac tttaccgaac gctctctgtt tgccgggtga caggataatg
    18661 acaatgttgt catttttgct gtaatccgtt gataacgatc gtgggcgcaa taaaatgaca
    18721 ttagcagcca gccggggagc atgatgaaaa tattaatcgt tgaagacgaa attaaaacag
    18781 gtgaatatct cagcaagggg ctgacagagg cggggttcgt cgtggatcac gctgataatg
    18841 gtctgaccgg atatcatctt gccatgacag ccgagtatga tttagtcatc ctggatatca
    18901 tgctgcctga tgtaaacggc tgggatatca tccgtatgct gcgcagtgcc ggaaagggga
    18961 tgccggtatt actgctgacg gcccttggca cgattgaaca cagggtcaaa ggacttgagc
    19021 tgggtgcgga cgattatctg gtgaagccct ttgcgtttgc tgaactgctc gcccgggtaa
    19081 gaaccctcct caggcgggga aacacgatga tcacggaaag ccagcttaaa gtggctgacc
    19141 tctcggttga tctcgtatcc agaaaagtca gccgcgccgg gaaccgcatt gtgctcacca
    19201 gtaaagagtt cagcttgctg gaattcttca ttcgccatca tggagaagtt cttccccgct
    19261 ccctgattgc ctctcaggtc tgggacatga attttgacag cgacactaac gcgattgatg
    19321 tcgcagtaaa gcgactccgc gctaaaatag acaacgatta cgggacaaaa ctgatccaga
    19381 cagtgcgggg cgtgggctac atgctggaga tcccggatgc atagcaaacc gtccagacga
    19441 cctttctcac tcgctctgcg gctgaccttt tttatcagcc tgtccacaat actggcgttt
    19501 atcgccttca cctggtttat gctgcattct gttgaaaagc attttgccga gcaggatgtc
    19561 agcgatctgc aacaaatcag caccacactg agccgtatac tccagtcccc ggcagatccg
    19621 gatgaaaaaa aagtaagcaa aataaaggaa tcgatcgcca gctaccgcaa cgttgccctt
    19681 ttgctcctca atccccgggg tgaggtgctg tacagctctg cgcagggagc agcactacgc
    19741 ccggccgtga attcagccga ttttagcgag cacagccgcg cacgggatgt ctttctctgg
    19801 acggtggagg atactgcgag agcgatggat accgggtccg gaatgaagat ggaaacgtac
    19861 cggattatcg cctcctctgg ccaggcgaca tttcagggca aacagcagaa ctatgtcatg
    19921 ctgactggcc tctccattaa tttccatctc cattacctcg atgcgctgaa aaaaaatctg
    19981 attgcgattg ccgtcgtgat aagcctgctg attgttctga tcattcgcat tgctgtccgt
    20041 caggggcacc tgccccttcg taatgtcagc aatgccatta aaaacatcac ctccgagaat
    20101 cttgatgcgc ggctggaacc gacacgcgtt cccattgagc tggagcagct ggttatctcg
    20161 ttcaatcata tgattggaaa gattgaggat gtttttaccc gccaggccaa tttctctgcc
    20221 gatatcgcgc acgagatcag aacacccatc accaatctgg tgacgcagac tgaaatcgct
    20281 ctgagtcagg atcgaacgca gaaggaactt gaggatgtcc tctattccag ccttgaagag
    20341 tataaccgga tgaccaaaat ggtcagcgac atgctgttcc tggcacaggc ggacaataat
    20401 cagctgatac ctgacagggt tcggtttgac ctcagagcag aagtcatgaa agtcttcgag
    20461 tttttcgaag cctgggccga agaacgcaat attatgctca aatttaacgg gatgccctgc
    20521 ctggttgagg gagatccaca aatgttcaga agagcgatca ataatctgtt atccaacgcc
    20581 ctgcgttata ccccagaggg acaggcaatc accgtctcaa taagagagca ggagagcttt
    20641 tttgaccttg tgattgaaaa tccggggaaa ccaatccctg aagagcattt atcaaggttg
    20701 tttgaccgtt tttatcgggt ggatccgtcc agacaacgaa aaggagaagg cagcggcatc
    20761 ggccttgcga ttgtgaagtc aatcgtggaa gcacatcacg gaagagtgca ggtggaatcg
    20821 gacgtacact caacgcgttt tatcttatcc gtgcccagac tggagaaaat gattccggac
    20881 acccagtgct gggaataaag atttaaatga caaagatgtc attagcctgt catgcagcaa
    20941 acagaagcca ttcgatataa ttagtgcaac ttatcaggaa ggctggattg ctttatcaat
    21001 atccggcgtc agtggacgcc aggaaatgaa tatccagtcc tctcccggag aaataaacac
    21061 ttccgaacct gtttcagtta tggaactgaa aacaccggtt gtactccccc ggacatcact
    21121 aattaagaaa tggagagtta tcatgaaaaa tatcgtatta gcatccttgc tgggctttgg
    21181 cttaatttct tcggcctggg ccactgaaac cgtgaatatc catgagcggg tcaacaatgc
    21241 acaggcacct gctcaccaga tgcagtctgc tgcggctcct gtcgggatcc aggggactgc
    21301 acctcgtatg gccggtatgg accagcatga acaggccatt attgctcatg aaaccatgac
    21361 gaacgggtcg gcggatgcgc accagaaaat ggtggaaagt catcagagga tgatgggaag
    21421 tcagaccgtt tcccctaccg ggccgtcgaa gtcattagcg gcaatgaatg agcatgaaag
    21481 agctgcagtt gcccatgaat ttatgaataa cggtcagtct ggcccacatc aggccatggc
    21541 cgaagcgcat cgtcgcatgc tcagtgcagg ctgacggcga gggaagtatc tgtgctgtcc
    21601 ttatttcctt gatagatttt tccgtaaccg gatatatggc aacatctttg ccccctacag
    21661 ggggcatttt tatcagattc tgctacagtt aacgggcatc aacgctaaaa ggatattttt
    21721 tatgaaagcc agaaacactt tatttgcagt attaatgtta tccctgccag cgatttcagc
    21781 agaacattca gaaatgaaaa tgactgatat gtctacctcg gcttcgtcac aggaatatat
    21841 ggctggcatg aaagacatgc atgacaaaat gatggctgcc gtaaatgagt ccgatcccga
    21901 caaggctttt gcgaaaggca tggtagcgca ccatgaaggg gcaatagcaa tggctgagac
    21961 cgagctcaaa tacggaaaag atccgaaaat gagaaagctc gcgcaggaca tcattaaagc
    22021 tcaaaaaggt gaaattgagc agatgaataa atggcttgat agtcaaaagt aaacattgta
    22081 gaaatgtcac gacaagcgtg attttacatc gttcctgggc gcgggagtcg gttaatcttt
    22141 atataattca aacccgatta attgcggatt tttactactc tgtactccgg tcctttgttg
    22201 aggaccggaa tataagcacc tgagatgctt atttaataca ttatctcgtc atggatacaa
    22261 tgtcgctcag gatctcaacc agctcgcttc tctcctgcgt tccgtcatga taggttgatc
    22321 ttgagatgat tctttctgat gtcatctgag gccagaattt atctagtaca ttatcgggtt
    22381 tcattcccac agcccggccg attatttttt tagcgtcaca ggtgtccgta aacgtgacgg
    22441 ctacaccagt aatttcctcg ataagatccg ggcacagata gttttctatc tcacgtttgc
    22501 gcgtagcgaa aaatacctta ccggcctcct ctacttcttt tttacgcttc tggatggaca
    22561 gaacctgtgc agggtctccc ccaatatcgg agtccagaaa tacgcaccag ggaagcccca
    22621 gatctttggc cagattcaat gtaacccagt gtttgacgct accacaccct cctatgagga
    22681 ctggcactat tttcacgtcc tccagagagg ctggcagcgc acctgactgt tttaatgaac
    22741 tggccgcatg cctcaggaaa gtaacatccg actttccctc taccagaaca atcccctgcg
    22801 ccctttccat accggtctct ggcagcaccc ccaggctttc agtggcttcc ttcagcactg
    22861 cgtcatccgg catttttact acgggttcac ccgcctcatt tcgggtaaca taacgtacgc
    22921 cttcgacagg gattaatccg gccagcgccg ggacatgggt ggtgacgata atctgacggt
    22981 gcggctggcc tgccagtgcc agtaacgctt tcatcagcat catctgatag ttcggatgct
    23041 gtgacgtttc aggctcctct atggcataaa tcacatttct gggcgtcccc gcgacattct
    23101 tttcagcctc agcccgaaaa aaattcaaca ggataagcct ccttatcccg ctgccgcgct
    23161 tattgatggg gatgccattt tccccgtcca gggtgaaatt gaaggtccac ttgggtttct
    23221 ccttaaatcg tggagtcagt tcactggcga gttcgggggc catttcacgt aatttatcca
    23281 gcgttctctg tgcgacatcc aggacgctgt cctgaatctc attttccagc gctgtaattt
    23341 tgtcctgcag cgcagcctga gcgtctttta cggcctgctg taaggggttt ttagcttcgg
    23401 aatccccgtc gctgctttcc ctgtcggctt tgaaaatcgc atacgtgggc agcaaatcga
    23461 ggatcttacc ccataatgat tttgtgtcgg ttccgaactc tttatcgaca tccagcataa
    23521 tttccgaaca ggtatagggg gctgcggctt ccctgatggc ctgacgccat aacgatgcag
    23581 tgcgtttatc cgccacattt ttttcaactt cttttcccac cgccttgagc tcagaaattt
    23641 tcatgcccag taaatttctc aggggctcct catccgggtg gatgcagcgg atgcaggtct
    23701 gctccggttt tcctgaggtc gttgctttaa acgttttgac gatttcaaaa ttgccgtcct
    23761 cattcagcag atgttctgag gcgagcgtgg tctgcaccct gtcgtcgatc atgataaaat
    23821 cgggcaggtc atcaaattca caggctattt caaaacggcc gtccccttct cttaggctga
    23881 aacagttcat gtcattcttg tcagccttca ttccttctgt ttcaaaaaaa atagccagcg
    23941 cttccagaag cgttgatttc ccaaagtcat tacgaccgac aatacccgtc atgctttcat
    24001 caataatgat ttcagtagag tttttgtagc ctctgaagtt tttcagtttt aattttctga
    24061 gccgcatatg aagtcctgtg tgtaagaaag cgttttgctg caacctgaat acaaaatata
    24121 ttttcaaatg cctgccaaca agcagggata accgttattc agaatatctt aacgacattt
    24181 cgggattatg atctggtcgc gctttcagaa ctttactgcc cctcaaaaca ccggaaacta
    24241 acttgtggtt ggttacatgg caaagttaaa actccagaat cacgggtaat gatttcaatg
    24301 cttattatat tttttggaat tgactcatga gctttataat ttaaatgata ataataacca
    24361 ttcttttatg cgtaatattt gctactgaca ctgatgaatt taaaagaaga tagattttca
    24421 tttacggatt atttcctaaa ttcatcgata atgtgatcga cataaaattt tcactgaaga
    24481 atgaagagat aatttaaggc cgagcgaaat agctcaaaat ggtgtattta acataacaga
    24541 agaaatgaaa ggctctatcg caacctgata cttcaggccg ctaaggggtt aatcttgttg
    24601 aagaaaaatt agaccgggaa gcgccaggcg tcaccatttc tcccgcctgg ctggaagtga
    24661 agaataattg tgcatagctc cgtattcggg ctttgcatct gacaatggtt ccctggatga
    24721 aagtacgatc tccactccaa ggacgctgaa caccctaaac aaacgctcaa aacccgcact
    24781 tgcagggttg gcctctagtc gggcataagt ttgctgtgtt atacccaaac gttcagaaac
    24841 atctttttgc gtcagtccat tggctttgcg aaagccgatt aaaacaggac gtaactgatt
    24901 caaagtcttc aaagcgtatt gagtgttcat aagtgacctt ccgtatcgac gggttaacaa
    24961 gcataacgca gggatttaca gcatataggc tgtaatgctc attaacagac tataggctgt
    25021 aatacgaatc ttgccaagat gcggcatctt tcaaatacaa ggccgtattg ggtaagcagt
    25081 acaccgttat atagcacccc ttgccccttt gctggagagg gacccacgtg atcgcaggtc
    25141 ttttagacgt tgttgctaac cggcatcata cacaggtggt aacccccagg gaaactctat
    25201 gagtgataat ttattcctgc cccaatcaaa atataaaacc ttatctaact agctttctta
    25261 gtattttata aatgaagtga aattagcgaa gaagttttac attgtaaagt gagcaaaaat
    25321 atttaccatt caactggcaa aatttacaat ctgcagccct tagcctgaga aaatgcacat
    25381 taacagaata atcatcaagt ccgatagagg cataacttac gtcctcaaaa atatcagaaa
    25441 taaatgagtt gaaattacaa aaattaactt cacttaacat cttgaacacc gcagaaatat
    25501 atctatcatg cattatcttg acttcgataa ccttagataa acttgaatgc gacttacatt
    25561 tgaagagatc aaagagaaag taaatcagca tatttttatt gtccgaaatc cattttaaaa
    25621 ttttattacg tgcacatggt aatatataca ctggattttt gaaagggttt gaaacgtcaa
    25681 ccacaaggga acaataacca tgaggattta ttccacttat aattaagtca ctaagtcctt
    25741 tcactgcatt caaatagtta tcaaaacaaa cctcatataa actggtacta tgaactgcac
    25801 atttagattc aaacaaccat ctatgacgca gataaccaat gcctttagag tgtatatctt
    25861 caacaataca tttgtgacag taacgaatgt ttgagatgtt taccaatgga tatgaattag
    25921 ttatgtgaaa atttttccga caggcatctt caacagcgtt aaaatacccg taaacaccag
    25981 aagataacga cactatattg gaagttagta tataaccatt tctagtggca agaatgtcat
    26041 atatatcctg caagctatgc aatttgtaca tatcaatcag ttcgaaacga ggaaaggcat
    26101 cgtaccttag tgcgccatgc tttgatatta tgttcctgca attggataag ttatacatta
    26161 tctgtgaacg cagaagaaaa ctgtgccaca attcattttc gtaaaccata taactccctc
    26221 ttatttaatt agttccattt ttctgagaag tatcattgtc attggtagtt tatctctgta
    26281 tttcaaaagc cagccatgcc ctcccagtaa agcatctaga cttgtaagtg acatcgagta
    26341 ctgcccctga gataaaacat catttattaa tgatgatgag ataatatctc tctcgctcca
    26401 gtctactctt ttatttttat agcatcgtat tgttttagga agacaggaat taagccagtc
    26461 acattcatgt ttatttagcc aatagaaact tgcataacaa ctttctttga ctaactttcg
    26521 tgttatagaa acggattgat tatgtataaa ccttaatatt ttctgcttat gacgccgtct
    26581 taaagaatct tttttacatt ttttacgcca tgagcataaa ccgtaacaag atgaaattac
    26641 agaggataca gctccttccg ataatgagtt tttctcagct atggatgcca gactaaaccc
    26701 ccttacagcc atagacttga taagcaattc agtgaatata ttaatttttg tgtttcgctt
    26761 aaacgccctc aggtattttt tataaataag tgttttaatg taacatctgc tgcgactaaa
    26821 ttccttccca aggttgctaa cactagtatt attttcaact atatgaaaac tgtatttctt
    26881 atgactttta aagatttctt tctttttatt agtacgactt cctgcatacg ttggccagca
    26941 agtatttaac aaacaataac aaagcaaaag atgctttgtg ggatgctgac aacatttgtc
    27001 tttgagtata ggctcccagt aatgataatc agttagggat gttggtataa gtcccgatga
    27061 accctcccca aataactggc acttagcata aacatgtgcg tagaatacat ttttctttaa
    27121 atttccatcc aatgataata aattcaactt tttaagtaca tccatgtaat tggggtgtgt
    27181 aatatctttt cttctgatta tatttaggga ctcataacaa aacttagcaa aatcgaagtc
    27241 tatctcattg ctaagttgtt ctgtatacga aacaggaggc atcagtccaa tacgtatgtg
    27301 cggtgaagaa gggacatgga tgctttcaag cgctaccgga tgccgatggc aggttgtaac
    27361 cccatgaagc tgatgcgcca gatgccaata agtaacacca taatgaaaag tatcttcacg
    27421 agcgcaaacc gggcaaaact tcaatagtgt gcgctgccct agtgaaaaat tactaaggcg
    27481 gcacaatcga ttaagcctgg caggggtcgt gttgaaatcc ataatttcat tacgactgat
    27541 tggtagtgcc caggcaaaaa gagggagtaa agtctgttca tgccagagct gatctgcact
    27601 ttcggatgta tgaagagaaa tagcctttaa tcctgaattg agaattggat ggacgttcat
    27661 atcaggtttg ttgaaaatga tcgttaacag agaagatggg gacataccgt acacggtagt
    27721 tgtccgacaa aaccggctga acaaggattc atcaggcaaa acaggcaaca ttctcatttc
    27781 ccacacctct ctttgaccgt tacgaactgt tacctcatta caatttatta agtggatcta
    27841 tatttataag cccgcaacgc tgaagagcat cgtaggtatt tttcttttca gctgttctgt
    27901 tgataacaag atcgtggtca agagacagta ttttctcgtc gaaattgcgc aaaacctcgt
    27961 ctatttttcc tacgaattcc ttgtgctgtg gcctgtctat atccccaggg atcaaagact
    28021 ttagggactt ctctgccatg attttagccg aagcatcacc ctcattagat gcagagagcc
    28081 atttagcttc ctcatcttca gtcgcggccc ccgggagtgg agtatcctta tattcctccg
    28141 tatgactcac gagtaccggt acagaggccg acaagactgc gccagtgatg atttcattgc
    28201 cgctaccgat aacctttaat tgtgcccccc gataaatcat ctgagccaat ccaatattgc
    28261 ctttagataa aatgaataat ttttcgttta actcatcatt taatggagtt tcaacatttg
    28321 tccattgtaa tggccataaa taatgaataa acgattgcca gtcttgtgaa tcgtatctaa
    28381 cattcttaat tttcatgtaa ccaccagact ctgcccgcct ggcaattctc attttctttg
    28441 tcagcgtctc gtcaaaagga tgatttccac aaaaaaccat agataccccc atggaatcta
    28501 caatttcatg taaaaaatca atcaacttac tctcaccccc ggttcttgaa aatttaagcc
    28561 tctgcatttc atcaataaca aggataccca aaaatgatga tttaattctt tgtgcaatct
    28621 ggcgagccag agcaccattt ctaatctcag gtgtagttcg ttcaatacca attgcatcat
    28681 ctaatttttg taaaatctct tcacaaaggt ctctcacact tgaattgtat ggacaattaa
    28741 tctttaccca tacaatttgc ttactgaagc cgggaaaaac tcctttatag ctgctgtgtt
    28801 ctataatctg agggaaatga tccatgactt gctcaatcat ggtagttttt ccagaaccac
    28861 tcattccaac tatcgtaata gtttcagcct gggatttaaa atatccactt tctggctcaa
    28921 tatcaggacg ctcatttcca tagtaatgaa gatattgaat tgttccgctt tttaaaggat
    28981 tacgagctgc atagctttcc ctaagtgccc gtaagatcat cttataagcc tggataagat
    29041 tggtttgagg gcaacggaat ttctttatac gttcgacata ctgctctcta acattcgccg
    29101 gggcagtcca gcggattttt tcgtcaattt cggggaaata actcatttcc agaagtactt
    29161 catcttccgt aagcgcttca ggaagtgctt caattaaggg attgccacga tactcaggta
    29221 actcctgttc tttatatatc gctgaaacgc gccgtataag gttcattatt ctgcgtcctc
    29281 agtcttcttc ttacctttta accatctttg tttctcctcc cggccaggca aaagcagaac
    29341 attactggat gaaggaagta ggggttcaga tcttccatta gcaacatgag ttcgccctaa
    29401 tgaatcaagt tcatttttcc tgtttttttt aatatcttta ccatgtaccc gacgagattc
    29461 gtatatttca tttaatcttt cgttaccttt cctgttaaat tcatctttat tattgatatt
    29521 gctgagcgaa tccagagtaa ataaattaat ttctttttga gtatctaccc aatcagccat
    29581 tacatctgta tcaagatgag ctttcccttt aaatacatct cttttcttaa gtaggtaatg
    29641 ttttttgaaa atgctatttt tgtcaaatct tacatatata taatctacgc aattgtcatc
    29701 tattcttgct tcacaagtgg ttcttccaaa tactctgaca cctgatgcaa tttcaggatc
    29761 acattcataa aaaagattat tgtactgaat cccaccggcg gtaatgttag cgttcacagg
    29821 gattaacaat cgtgcgatca cttcgtcggc cccaacggct cttgcgctga atctcccgtg
    29881 ctttaagctt atcatccaac tattttttgg tgatattacg agatcatttt caatcagtaa
    29941 gggattgata taagccagtt cctcatacgt tctctgatta tgtgcaagta tctcccgaat
    30001 cagtaaagac gtgacctcct gaatcgttaa acaagccctg gattgcggtg ttggctctcc
    30061 tttaacaatc tttccccttc gtgttgttcc aataagccta tgaataacct catcattcag
    30121 aatgccgaaa cagcgttcaa caatgctttt tctgtcgcct ctaccgactg gcgcaaactc
    30181 aagttttgtc aggggggtca tctcttcctg aggtttaaga ccaatcatct ccccgttgtc
    30241 acacatcaat gttaatggaa tgtgagaaca aggccaatca tcatcagtaa tagagattcc
    30301 aaataatcta caatattctt tttttggcat aaagcaattt gctaacgctt gtcgggcggc
    30361 tcgccatgaa gcatggtata aagaaacatg aaggccaaca atcattcttg ttgcacgatc
    30421 caccactgta taaatagtag gacgcccaat gacttttcgt ctgtttaaac tcgaaataag
    30481 atgtacatca gcgacagttg agtctatttc gaaaacatca cccggcaaaa cagttgtatt
    30541 agccacacta cctaaaagcg cccgcatttt gaggtctatt tcttttttcg tgttcttctt
    30601 atttatcgag aaatctttag tgaagagttt tttattccag tatgaaaatt gttttaaaga
    30661 aggaacatat ggagctctat tttctaggtt tgctgtttta atttcatccc taaaatacaa
    30721 atcaatatgt ctctctaatg ttttctttat cgtatctccg ttaactttat aatgatacgt
    30781 aatgagagat tttctaatat tatttatatc tctttcatta agaatgaaaa cacgtgatcg
    30841 ttcattaggt aatgctctgt tttttttgga attcccaagt ttaatttcat gtttgattct
    30901 acttttccca gcggcgccac agttcgagaa tgcgggtagc aatgcataaa tatcctgccc
    30961 atgtcgccaa tacaacgcca gaagcgttct gatagtatat tgtgatattt ttttatttct
    31021 ggagtaatcc attaaaaggt gcgacttttt atgtaatgca tagtcgaaca gaaacattct
    31081 gtcatctaca agatctttaa tgatattgta attttcatct cgccttcctc tgtctttatc
    31141 tgttaattct ttctcatcta ctaacatata tgaaggatat atataatctt tacaagttat
    31201 gctatttgat tcaatataat atttaaacat tgaaaatgag aaagggattg gcttacccgt
    31261 tctttctgat tttattttaa acaaaattaa caaatcgacc tttctgtcaa aatccaaaac
    31321 acggtaaagt ccctctgcaa gcaaatcagt gccttgagag agccagactg aattcctaat
    31381 aatatccatt tgcgttcagt cctatatagt ggtcattgga aattatcttt atcagaccgg
    31441 tttcgagtat tggatatgat aaatcaacat ttatcatttt taatccaatc attgcctgca
    31501 ataacatcat cgcttcttgt gcatctaccc caaagattga tgaaatcatt gcacgtagtc
    31561 ctactatggg ataaacatct ggtttaagct tccataggat tttatcaaga ggatatttat
    31621 cataaaattc agaaccatct cttaatacag aagtagccca gcaaatgttt ttaccgacga
    31681 cgttattgag ttccgagcca acataaattt gaaactcaac atcaatcaac tgccaaaaca
    31741 tcctctctat ttcaagtttt tgggcttcgc gttcaggtat gctctcgtta tgttttactg
    31801 ctatagcttt gtatcgaagt ccgccttcag gagttctcaa ggtcagtaaa aaatcggttg
    31861 tcataacatt cagaacttcg acaggtactc ctcttactcc cctcaccata ggatgttgaa
    31921 aatgtagatg atttgctatt tgttgggtaa gccggagagg aaagagtggg aattgttccc
    31981 ggatatcaat caccgagtca ttaaactcag ccagatagaa aaagttactt tcgacagaag
    32041 aaaggagatg atggtttcga aacgtcttaa ggccaaagac tatcgaacgg tttccacggg
    32101 atttaacgtc ctgagctcta agccagggct gataactttg gccatagccc actcctatgc
    32161 cgtcacctag cgcattttca taatccaaat aggatttgag acgcctaccc ctagccatga
    32221 tcgtacccta aaaacgacat tgatacgtac ctatatgtgc attttatagt ctccaaccct
    32281 ataaccacac attcagtaag tatacaacat tagcgtcgtt ttttgagaat ggttacaact
    32341 ttagtgcttt gtgta
//
LOCUS       DAOFQD010000050        32266 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.050, whole genome shotgun sequence.
ACCESSION   DAOFQD010000050 DAOFQD010000000
VERSION     DAOFQD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.050"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(53..1450)
                     /gene="ccp"
                     /locus_tag="QBF46_002316"
     CDS             complement(53..1450)
                     /gene="ccp"
                     /locus_tag="QBF46_002316"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDQ6674492.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1855..3504
                     /locus_tag="QBF46_002317"
     CDS             1855..3504
                     /locus_tag="QBF46_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDQ6674493.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKENIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3555..4157)
                     /locus_tag="QBF46_002318"
     CDS             complement(3555..4157)
                     /locus_tag="QBF46_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDQ6674494.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4677..5576
                     /locus_tag="QBF46_002319"
     CDS             4677..5576
                     /locus_tag="QBF46_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6674495.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5625..6638
                     /gene="yhjD"
                     /locus_tag="QBF46_002320"
     CDS             5625..6638
                     /gene="yhjD"
                     /locus_tag="QBF46_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDQ6674496.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWISTAEYKDDPRMPGK
                     TQP"
     gene            7049..8371
                     /locus_tag="QBF46_002321"
     CDS             7049..8371
                     /locus_tag="QBF46_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDQ6674497.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8553..10613)
                     /locus_tag="QBF46_002322"
     CDS             complement(8553..10613)
                     /locus_tag="QBF46_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDQ6674498.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVIGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10683..11450)
                     /gene="pdeH"
                     /locus_tag="QBF46_002323"
     CDS             complement(10683..11450)
                     /gene="pdeH"
                     /locus_tag="QBF46_002323"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDQ6674499.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11682..12611
                     /gene="kdgK"
                     /locus_tag="QBF46_002324"
     CDS             11682..12611
                     /gene="kdgK"
                     /locus_tag="QBF46_002324"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDQ6674500.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAAKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12707..14203)
                     /locus_tag="QBF46_002325"
     CDS             complement(12707..14203)
                     /locus_tag="QBF46_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDQ6674501.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14424..15710)
                     /gene="dctA"
                     /locus_tag="QBF46_002326"
     CDS             complement(14424..15710)
                     /gene="dctA"
                     /locus_tag="QBF46_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDQ6674502.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15893..17881)
                     /gene="hmsP"
                     /locus_tag="QBF46_002327"
     CDS             complement(15893..17881)
                     /gene="hmsP"
                     /locus_tag="QBF46_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDQ6674503.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAKELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17963..21436)
                     /gene="bcsC"
                     /locus_tag="QBF46_002328"
     CDS             complement(17963..21436)
                     /gene="bcsC"
                     /locus_tag="QBF46_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDQ6674504.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQSDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKSNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21418..22524)
                     /gene="bcsZ"
                     /locus_tag="QBF46_002329"
     CDS             complement(21418..22524)
                     /gene="bcsZ"
                     /locus_tag="QBF46_002329"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDQ6674505.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFSALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22531..24870)
                     /gene="bcsB"
                     /locus_tag="QBF46_002330"
     CDS             complement(22531..24870)
                     /gene="bcsB"
                     /locus_tag="QBF46_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDQ6674506.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24881..27499)
                     /gene="bcsA"
                     /locus_tag="QBF46_002331"
     CDS             complement(24881..27499)
                     /gene="bcsA"
                     /locus_tag="QBF46_002331"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDQ6674507.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIVLFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27496..28238)
                     /gene="bcsQ"
                     /locus_tag="QBF46_002332"
                     /pseudo
     CDS             complement(27496..28238)
                     /gene="bcsQ"
                     /locus_tag="QBF46_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            complement(28250..28438)
                     /locus_tag="QBF46_002333"
     CDS             complement(28250..28438)
                     /locus_tag="QBF46_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDQ6674508.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28711..30282
                     /gene="bcsE"
                     /locus_tag="QBF46_002334"
     CDS             28711..30282
                     /gene="bcsE"
                     /locus_tag="QBF46_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDQ6674509.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHNGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30279..30470
                     /gene="bcsF"
                     /locus_tag="QBF46_002335"
     CDS             30279..30470
                     /gene="bcsF"
                     /locus_tag="QBF46_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDQ6674510.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30467..32146
                     /gene="bcsG"
                     /locus_tag="QBF46_002336"
     CDS             30467..32146
                     /gene="bcsG"
                     /locus_tag="QBF46_002336"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDQ6674511.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNILTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLISHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
BASE COUNT         7608 a         8836 c         8114 g         7708 t
ORIGIN      
        1 tattatcgta ataaaaatcg cccaaacatt cctgttcggg cgatcaaatt aattattgtt
       61 tatcctgcat atacggcgtg tacaccccgt tcagactgtg caggaaagct acgatatcat
      121 ccacatcctc ctgcggcagc tctttgccta cctgatagcg cagcatcagt ttcaccgccc
      181 cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc cgttaaagca acgttacgta
      241 aaccgggtac tttctgacgc aatttatcac gctcttcttt agtaacattc atacgaccaa
      301 tatccgccgc cgtaatttcc ccaaagttaa aatctttttt cagccccaac ggttcaaagg
      361 aacgtccacc gagaataata ccaccatgac aagttgcaca tttattatct ttaaataatt
      421 gatagccttt tttctgttgc gccgtcagcg cattttcatc tccacgcaac catttatcaa
      481 atggggaatc cggcgtaatt aatgttttct caaattcagc aatggcatca gtaatatttt
      541 cgccactgaa accttgcgga tagacgccga ggaactgcgc tttaagctgc ggatcttttt
      601 ccagcttagc aataatttcg tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg
      661 gtccaccagc ctgatcctgc aatgttgccg cacgaccatc ccagaactgc tcaacgttaa
      721 atactgagtt aaataccgtc ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg
      781 atgtttttct gccatcgacg ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg
      841 tgctatcagc cgataaacgg ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg
      901 catcggtagg cagtttttgc gggatgggct gcaccggttc attgcgatgc tccggagcag
      961 tatcattgct ggcgtaatat tccgcgcgct gttttgcaat ccaggccagt atttccgccc
     1021 gctcttcatc actcacctta cccgcccagt gtagcgcggt ataacgcgtt ggtggcatag
     1081 tttcatactg catcacccat tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag
     1141 ccagcagtgc cgcacgcacg gcctcgaggt taaaagattt atatccaagc ttaatgtcgt
     1201 aatccatcaa ctgtttcgcg ccaggaatat aataataggc gggtaattct gccgaaggcg
     1261 tgtggcaata gtcgcatcct ttttcgcgga gaaagcctaa aaccttatta ttttcactga
     1321 cagcagatgc ctgaacatcg gcctgtttac tgcgtttatt atcgtggtac cacacataac
     1381 cagataaccc taaatagcaa atcgcgacgc cagccaggcc gatcgcggta atacgtgaga
     1441 ccattttcat tattcttccc tcacggtggt gagttattgt gacccagaaa attttccaga
     1501 atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa acagtgagat acgctttttg
     1561 ataggagtca atcaattaca tgaatataaa ctatcaataa gatagcctgt gcctattacc
     1621 cagatcagga atggcatatc atgattgcgg cgagagcagg attgaatgtt ctgaaaatga
     1681 aaaacaacac gaaatccgta tttttataaa ttccatggat attagtgcgt tatatttttt
     1741 gacaggttaa taaacgtgat ctaccgcacg ctttgtcgcc caccaggcgg agcgaatgac
     1801 tacccttaaa gaaaagcccg ataattagcg acgaatttcg gaggttggat ccttatgctc
     1861 aatcagaaaa ttcaaaaccc taatccagac gaactgatga tcgaagtcga tctctgctat
     1921 gagctggacc cgtatgaatt aaaactggat gagatgatcg aggcagaacc ggaacccgag
     1981 atgattgaag ggctgcccgc ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg
     2041 ttcgagcatg ttcagtcggc gaaaattttc cccgacagta aaacctttcc cgactgcgca
     2101 cccaaaatgg acccgctgga tattttaatc cgctaccgta aagtgcgccg tcatcgtgat
     2161 tttgacttgc gcaagtttgt tgaaaatcac ttctggctgc cggaggtcta ctccagcgag
     2221 tatgtatcgg acccgcaaaa ttccctgaaa gagaatatcg accagctgtg gccggtgcta
     2281 acccgcgaac cacaggatca cattccgtgg tcttctctac tggcgctgcc gcagtcatat
     2341 attgtcccgg gcggccgttt tagcgaaacc tactattggg actcctattt caccatgctg
     2401 gggctggcgg aaagtggtcg ggaagatttg ctgaaatgca tggccgataa cttcgcctgg
     2461 atgatcgaaa actatggtca catccccaac ggcaaccgca cctattattt gagccgatcg
     2521 caaccaccgg tttttgcgct gatggtggag ttgtttgaag aagatggtgt acgcggtgcg
     2581 cgccgctatc tcgaccacct taaaatggaa tatgccttct ggatggacgg tgcagaatcg
     2641 ttgatcccta atcaggccta tcgccatgtt gtgcggatgc cggacggatc gctgctcaac
     2701 cgttattggg acgatcgcga cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc
     2761 gcgaaacatt ctggtcgccc gcccaacgag gtgtaccgcg atttacgcgc gggagcggcc
     2821 tcaggttggg attactcttc ccgttggctg cgtgatactg gtcgtctggc gagcattcgt
     2881 accacccagt tcatccccat cgatctgaat gccttcctgt ttaaactgga gagcgccatc
     2941 gccaacatct cggcgctgaa aggcgagaaa gagacagaag cgctgttccg ccagaaggcc
     3001 agtgcccgtc gcgatgcggt aaaccgttac ctctgggatg atgaaaacgg catctaccgc
     3061 gattacgact ggcgacgcga acaactggcg ctgttttccg ctgccgccat tgtaccgctc
     3121 tatgtcggca tggcgaacca tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg
     3181 ttactgacac ctggcgggat tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa
     3241 cccaatggct gggcaccgtt acaatggatg gcaattcagg gatttaaaat gtatggcgat
     3301 gaccttctgg gtgatgaaat cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg
     3361 gaacagcaca aaatgatcga gaaataccat attgccgatg gtgttccccg cgaaggcggc
     3421 ggtggcgagt atccgttgca ggatgggttt ggctggacta acggtgtggt acgccgttta
     3481 attggtttgt acggcgaacc ataatatttt tacagccagc cgctaacttc ctgctggctg
     3541 taaaattatc ctcttcagga ggagatattt aacatcattg ccgcctgggt gcgatttttc
     3601 acttccagac ggcgatacag ggattccaga tgcgctttta ccgttccggt actgatattc
     3661 agcgctctgc cgatctcctt atttgattcg cccgccgcta acatggttaa aatctcccgc
     3721 tggcgggcgc ttaacgattt gagatcttta atgtcctttt ccggcgtcgt ccgccagtct
     3781 ccaggcagaa acatcatccc catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg
     3841 gttgaatcac gaggcacaat ggccagcaca ttaaaatgga taacttcctg taaccaccgt
     3901 ttattgcaat ccgtcgccgt gattaacacc ttaacctcag gaaactgcac cacggttttt
     3961 tgcagcaacc agtagcaaaa ctcaccatcc tgatcgccat cgagcataac taaggcttca
     4021 gggtaacttt ccagcttttg ccataactcg tctgcctgac tggccccctg aatactcact
     4081 cctggaatac gctgctgtaa actgattttc attccatgaa taaatattga ctgcctgtca
     4141 aacatgacta tttgcataac tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg
     4201 gaagggtatt acccgcgaga aaaaataaga attcgccatt tggcggtggc cattctacag
     4261 agatgacgcg tagaaaatag ttaccgatat aaatagttac agctaaacgc ctgaaattac
     4321 atgtcgaggg cactatttaa aacaattttg aggatttcct tatattggtg gttagtacgc
     4381 atgcaattaa aaatgaaact ccgcgaccac aagcaaaaat aacaaacggc aatgagacaa
     4441 aaataagcac aaatagccaa cacgtcctct attcacttta aagggaatcg ctgaaaaata
     4501 cgctctgttt agggggattc acctttctca gaaagctatt ccgccctttt cctgctgaga
     4561 aatcgccaca ttcgtcatga caacattgtg aaacccggca ttagatgtta gaaaaaacaa
     4621 taaacaatgc gatatgcgcg ttactgtttc gctgtgaaca acaatcggtc aaagaaatgg
     4681 ataaaattca cgcaatgcag ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg
     4741 cggcggattt ctttgctttg ccaaaaggaa gtgtttcgcg ccagatacag gcactggaac
     4801 atcaacttgg cacccagctt ctccagcgca ccacgcgacg ggtcaaactc acgccagaag
     4861 gcatgaccta ttatcaacga gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc
     4921 tgtttcaaca ggatgccacc agtatcagcg gtaaattacg catcgacatc ccgccaggaa
     4981 tcgcgaaaag cctgttactg ccgcgcctgt cggaatttct ctatctgcat ccgggaattg
     5041 agctggaact gagtagccat gaccgtccgg tagatattct tcatgatggt tttgattgcg
     5101 tgatacgcac tggcgcgtta ccggaagatg gcgttatcgc ccgtcccctc ggcaaactga
     5161 ccatggtcaa ctgtgccagt ccgcactatc tgacgcgctt tggttatccg caaagccccg
     5221 atgatctgac ttcacacgca atagtgcgtt atacaccgca cctgggtgta catccgttag
     5281 gttttgaggt tgccagcgtt aatggcgtcc agtggtttaa gtctggcggc atgttgacgg
     5341 taaacagtag cgaaaactat ctcgccgccg gtcttgccgg tctggggatt attcagatcc
     5401 cgcgcattgc cgtgcgcgaa gccctgcgtg ccgggcggct tattgaagtg ttgccaggct
     5461 accgtgccga gccgctctcc ctttcgctgg tttacccgca gcgtcgggag ctttcccggc
     5521 gtgtaaacct gtttatgcag tggctggctg gcgtaatgaa agagcacctg gactgaccga
     5581 ctatactttt taagaatgac cacgacagaa ggacaaaaga gcggatgacg caggaaaacg
     5641 agatcaaacg tcccacccag gatctggagc acgagccgat taagcagttg gataatagcg
     5701 agaaaggcgg caaagttagc caggcgctgg aaaccgtcac caccactgcc gaaaaagtcc
     5761 aacgccaacc ggttattgcg cacctgattc gcgcgacaga acgctttaac gatcggctgg
     5821 gtaaccagtt tggtgcggct atcacctatt tctcattttt gtcgatgatc ccgattttga
     5881 tggtgtcgtt tgccgcaggg ggctttgtgc tggcctccca tccgatgttg ctacaggata
     5941 tcttcgacaa aattctgcaa aacatcagcg atccgacgct agccgccaca ttgaaaaaca
     6001 ccatcaacac cgccgttcag cagcgtacga ctgtagggct tgtcggcctg gcggtggcac
     6061 tttattccgg catcaactgg atgggtaacc tgcgtgaagc gattcgtgcc cagtcacgcg
     6121 atgtctggga acgctcgccg caagatcagg agaagttctg ggtaaaatat ctgcgtgatt
     6181 ttatttcgct gattggtttg ttgattgcgc tgattgtgac gctttcgatc acctcggttg
     6241 ccggttcggc gcagcaaatg attattagcg ccctgcacct gaacagcatt gagtggctga
     6301 aaccgacgtg gcggttgatt ggcctggcga tttccatctt cgccaactat ctgcttttct
     6361 tctggatctt ctggcggttg ccgcgccacc gtccgcgcaa aaaagccctg attcgcggaa
     6421 catttctggc tgctattggt tttgaagtga ttaaaatcgt gatgacctac accctgccat
     6481 cgctgatgaa atccccctct ggcgcagcat ttggttccgt gctggggctg atggcatttt
     6541 tctacttctt cgcccgtttg acgctgtttt gcgcggcgtg gatttccacc gccgaatata
     6601 aagacgaccc gcgaatgccg gggaaaacgc agccttaaaa taggccggat gcggcgtaaa
     6661 cgccttatcc ggcatacgat tttattcggc ctggctcccc gtaggccgga taagatgcgc
     6721 cagcatcgca tccggctata atgcgcacat aacctcttga aactcttccc cagagcctct
     6781 tcagccatct attagggagc aaacaatttc ataccaactc ataaccccag catataaatc
     6841 cagttggtaa cttttattta acctgaaacc agttttatcc actatttata aaattatgtg
     6901 aagcatttca tagaagaaaa atcactggcc taaacattat cccctttttg cctggttttt
     6961 gaccatttcc gcgatttgtt acacattgaa atatcacttt tgctgtgcgt aatatggcta
     7021 ttcgttagcc aaaaaataag aaaagattat gcaagcaaca gccacaacac tcgaccacga
     7081 gcaagaatac acgccgatca actcgcgtaa taaagtcctt gtcgcctctc tcattggcac
     7141 ggccattgag ttcttcgact tttatattta cgccactgcg gccgttattg tgtttccgca
     7201 tatcttcttc ccgcagggcg atcctacggc agcaacgcta cagtcgctcg ccaccttcgc
     7261 catcgccttc gtcgcgcgcc ccattggctc tgccgtattt ggtcattttg gcgatcgcgt
     7321 tgggcgtaaa gcgacgctgg tcgcctcgtt gctaacgatg gggatttcga ccgtggtgat
     7381 tggtctgctg ccgggctatg ccacgattgg tattttcgcc ccgctgctgc tggcgctggc
     7441 tcgatttggt cagggtctgg gcttaggcgg tgaatggggc ggcgcggcgc tgctggcgac
     7501 tgaaaacgcc ccaccgcgca aacgtgcact gtatggctcc tttccgcagc tgggcgcacc
     7561 gattggcttc ttctttgcca atggcacttt cttgctgctt tcctggctac tgaccgacga
     7621 gcagtttatg agctggggct ggcgtgtgcc atttatcttc tcggcggtgc tggtcattat
     7681 cggcctgtat gttcgcgtgt cgctgcatga gtcgccggtg tttgagaaag tcgctaaagc
     7741 gaaaaaacag gtgaagatcc cgctgggtac gctgctgacc aaacatgttc gcgtaaccgt
     7801 actgggtacg ttcattatgc tggcaaccta tacgctgttt tacatcatga cggtctactc
     7861 tatgaccttc agtaccgccg ccgcgccagt tgggcttggc ctgccgcgta acgaagtgtt
     7921 gtggatgctg atgatggcag ttattggttt tggcgtgatg gtgccagtcg ccggattact
     7981 ggctgatgcc tttggtcgcc gtaaaagcat ggtaattatc accacgctga tcatcctgtt
     8041 cgcgctgttc gcctttaacc cgctgctcgg ttctggcaac ccgattctgg tttttgcctt
     8101 cctgctgttg gggttaagtc tgatggggct gaccttcggg ccgatgggcg cgctgttacc
     8161 agagctgttc ccaacagaag tgcgttacac cggagcatca ttctcttaca acgtagcgtc
     8221 gattctcggg gcttccgttg cgccgtatat cgcagcctgg ttgcagacta actacgggtt
     8281 aggtgcggtg gggttatatc tggcggcgat ggctggcttg acgttaatcg ccctgctgct
     8341 gacccatgag acgcgacatc agtcgttgta ataagagtga aaagcctgat gcgctacgct
     8401 tatcaggcct gaatttccat acaatatatt gaattcgcaa ggatttgtag gccggataag
     8461 gcgttcacgc cgcatccggc atgaacaaag cacactttgc caacaatcta aatctgttta
     8521 attgccggat gtcagacatc cggcaattat catcacttct tcatctgcga caaaatcgtc
     8581 cgacactgat tagcctcccc ttcggaaggt gagatcagtg ccagtaacgc cgccgccggt
     8641 gttaccagcg ttgccagtgc cgcagcaaca gcaccacggg caatcagcgg tccggctttc
     8701 accccagcct gcggattttt aaacgtcccc cgcacataca gcggcgaacg cagtgtgata
     8761 atccgaattc ctttactctc cggatcaata gtcaaatcca gctgttccga agcaaaactt
     8821 gccgtgccga taacattaat caacgcgttc tcagtatcga aagcaaaaat ctgcgggcgc
     8881 gccacgccgt tggcaatatt cagattcgcc gccgcgcagt tcacccgcac ctcatcatca
     8941 ccaaatatcg caccgacaat gtagttgccg acattcagcc caacaatctc catcaggttg
     9001 cggctcacca gcccgtcatt catcaacagt ttcaggttgc cgttactgtt gcctaaaagt
     9061 gccgccaccg agttaccgct accgcgtagt tccgcgtcac cgttcatttc ccccagcgtc
     9121 ttctgcatca gttccacatc gggcatcagt tctttcagtt tcagtcgacg agcctgaata
     9181 tctgcccgcc cctgcatcgg ctttttatcg ccttccagat ggatattcgc cgcaatgctg
     9241 ccgcccgcca tgccaaattt cagcggttgc aggcgcaggt cagcattttt gaggatgata
     9301 tgagtagaaa gatcgctaat cggcaggcta ctgccatgct caatgcgccg ccctttgaag
     9361 cgaacatcgg catccataac gtcccattta tcggtttcga agcggtcata aggcagcact
     9421 ttgcccgcag gctgaacgct tttttcgccc ttcttctgtt cagaccgttt cgacttttct
     9481 gcccctttcc cggagtcaac gccaattaac ggtcccaggt ccgccagccg caattgccgc
     9541 gactcgacat caccttccag ttttggtcgt ggctttccgg tggtgtagac cagagaaccg
     9601 tggatatcgc tatcgccaat tcgcccatta aaaccgcgat aatcaaagac cgacgatttt
     9661 tcagtgtcga ttttcgctac cagccgacca tccgtttcaa acggcggggt atcgggcagc
     9721 agaacgcccg tcagttcata gagatcaccc agtgaatcgc cagaaaattt aagccgtaaa
     9781 tcgacaccgc ccatcttcat tgggtcattc acgacgccat caaaagcaac acgggtatta
     9841 cctgaacgga aatcagcctg taccggaaac ggcgtccctt ctccacgcag cgccagcata
     9901 ccgcctattt ttcccgtacc cgtgagcggt tcaccgttat aacgtccctg cgccttcagg
     9961 ccaaaaacgt aatcgcccac cttttcttta tccgctttac ctttcgatcc agtaacttcg
    10021 ctgaatggca gcggcttgcc taacggatca acaaaaattt ccagatccgc tttgcttact
    10081 ttgtcatcaa tggcgatccg cccttgatcg aaaagaatat tatccagccg aaacgaccat
    10141 gccgacggct ttgcattcgc gtctttgtta tcatcgttgg caagattaaa cgtccagtta
    10201 ttgttctttt cagagaggcg aatcaggcgc gcgtcgggct tttcgagctt gatccacggc
    10261 agccagaccg ttttggtcag cagcgccagc ggggccagcg ttgcctctac gcgtggcaaa
    10321 tgcaccatcg tgacttcggg aatatccggt ggattgccaa gaatgatgtc ttccgcgtgt
    10381 acatggggcc acggcaccca gctgcgccag ccagtttctt gtttttgccg ctcccacacc
    10441 acgcccagat cgccacggat agcgaacgga cgattcaact ccgcagagac tttctggttg
    10501 atagtcggtt tgagtcgatt ccagtcaaaa gttgcaatca aaatgatcgc cacgacaatc
    10561 aacaacaaga aagcccctga aatcgcagcg gttattttgc ctgccttgct catcgttcgc
    10621 ttcctcatat tcttcctgtg ccagtcctaa agatagtcca gccaggcgga aaatgaggca
    10681 gcttatagcg ccagaaccgc cgtattcagc gtttctatcg gtgccgggcg tgaaagaaac
    10741 cagccttgtg cggcgaatgc gggcgagttc tgaacatcac gccactcttc cggcgtttct
    10801 acgccctcga caatcacccc gcgacaatag cgattcatta gatgtaaaag ctgagaaaag
    10861 agtgtgcgtc cttccggcga ctgacgcagc atcacaaaca gttctcgcgc gattttgatg
    10921 tagtcataac gcacttcact tagcgcagag aaatttgcca tcccggtacc aaaatcatcc
    10981 agccacagcg ggccaaattc acacatcgag gcaaaggttg aatctttcgg caggcggatg
    11041 tgttccacca gttcgaaacg cagccaggga agacgctcaa tctggcgcag gatttttggt
    11101 tgctgacgca gggcgatgag cgtagggcca tcaatattga ccgatgccag caggccgtgc
    11161 tctataaaga agtcggcttt ttgcgccaac aaatcaatct gctctttcac aacctccatc
    11221 cgatggctga cggtgatttc agtaaaatag cgatccggcg gcaggcgttg cgaaggattc
    11281 aagggatgcg tgaccaccgt taatagctcc acggccatta accgcccgca tgtttgatag
    11341 atcggctgcc aggtgtaagc acgctcacac tgcaaccaaa aacgccgttc ctgcaagctc
    11401 tcgatgcttg cttcagggtt gcttattcgc tggataacct gccttatcat caaagatgtc
    11461 ctgtgtttaa aagtgatgct gcccggactc gacaaagatt atcggcgcgt aagggcagaa
    11521 ctttaccgcc tggccggggt aaatttcctc actccacaac agaatctttc aggaatacgt
    11581 cctggctcaa aaaaataatg gaacactgtt ttaatatggt tgaccagcaa accaccacag
    11641 cgcaaactaa cgctaatttt ttacagatca ggttcacgac tatgtccaaa aagattgccg
    11701 tgattggcga atgcatgatt gagctttccg agaaaggcgc ggacgttaag cgcggtttcg
    11761 gcggcgatac cctgaacact tccgtctata tcgcccgtca ggttgatcct gcggcattaa
    11821 ccgttcatta cgtaacggcg ctgggaacgg acagttttag ccagcagatg ctggacgcct
    11881 ggcacggcga gaacgttgat acttccctga cccaacggat ggaaaaccgt cttccgggcc
    11941 tttactacat tgaaaccgac agcaccggcg aacgtacgtt ctactactgg cggaacgaag
    12001 ccgccgccaa attctggctg gagagtgagc agtctgcggc gatttgcgaa gagctggcga
    12061 atttcgatta tctctacctg agcgggatta gcctggcgat cttaagcccg accagccgcg
    12121 aaaagctgct ttccctgctg cgcgaatgcc gcgccaaagg cggaaaagtg attttcgaca
    12181 ataactatcg tccgcgcctg tgggccagca aagaagagac acagcaggtg taccagcaaa
    12241 tgctggaatg cacggatatc gccttcctga cgctggacga cgaagacgcg ctgtggggtc
    12301 aacagccggt ggaagacgtc attgcgcgca cccataacgc gggcgtgaaa gaagtggtgg
    12361 tgaaacgcgg ggcggattct tgcctggtgt ccattgcagg cgaagggtta gtggatgttc
    12421 cggcggcgaa actgccgaaa gaaaaagtga tcgataccac cgcagctggc gactctttca
    12481 gcgccggtta tctggcggta cgtttgacgg gcggcagtgc ggaaaacgcg gcgaaacgtg
    12541 ggcacctgac cgcaagtacc gttattcagt atcgcggcgc gattatcccg cgtgaggcga
    12601 tgccagcgta aacgcagcat atgcaaaagg ctggataaag tgttacccct ctatccagct
    12661 catccataaa tacctgatgg ttgtgctaac tcatcaccat caggcattac tgtgcaggtg
    12721 gaatatccgt cacttcagga tgtacgtcat ccgtggcgac agaggtggcg gcagcagcag
    12781 tcgatggggc catgatttga tcccaggccg cctgcaacgc tttcatgtta aattccggct
    12841 cgcctttcgg ctgcagcagt attaacgcca tatcattcga caactgctga cgcagatcct
    12901 gatttaacat ctccaccgtc aggctgttaa ggaaatcctg gcgcagtttc tgatattgct
    12961 ccggcgcgat atcgacaacc tgattttgca acgaacgcat ccgctgaccc atcagaatat
    13021 cggtatcagc tcgtgcatag gcggcaaaca gtttctgcag ctccagtttc ttttgcgcca
    13081 ctaacgcatt gaactcttct tccggcagac ctttatcgcg aaccttcgcc agttcacgcg
    13141 ccaccagatt aaggttgctg ttcagcttgt cgtttggtga ttcgatgttg atggcacact
    13201 gcgcacgcag atacagcaca cggcagtcaa atccaagacc gatgtctttg ctgttactgg
    13261 cgcttaacgc ttgctgaaca tgccagaaca gcgcctcgcg ggccaggtcc gcacgccagt
    13321 agcgcagcag tgcggctgat tcacgaatcg gctgccacgg cgtatcccac atgatagata
    13381 accggtcctg acgcaccgcg tcagtcataa tactcaccgc ttccgcacgc agcggagaaa
    13441 gcgtcggcac cggagctggc gtttcacgtt tgcctttcag ttcgccaaac gttttgttta
    13501 tttggtcgac aaccgagcgc gcatccacgt ttcccaccac cagtagcgtc attgcatccg
    13561 gggtgtacca tttctgatag aaatctttaa ttttttccgc ttcaacgggt tgtttcagcg
    13621 gatcggcagg atcgtgacct aacaaggttg accctttcag acgatagcgc caccagccct
    13681 ctttagtatc ggcaggccag gttgccacca tgtcctgact ttgcagcgcg tggttgatgg
    13741 tttctggtgt gatggtcaat ttgccagtgg catttgccag ataagagagc gcttctttca
    13801 gcaaatcgtt acggttattg ggcaaactca gattaaacag cgtggtgtca taagagacaa
    13861 ttaccggcgg catcgggcgt ttagggtcga tcccctgctg ccacaatgaa cgcgcctgtg
    13921 ctgcgtcaag gccaccgctt tgcgttagcg caatacgagg gatggcgtga ctgtaaccgc
    13981 tctgttgtgt actttcggcg agcgaaccgg tattaaccag caggcgaatt tcaacacgat
    14041 cgctgggacg ctggggggtg gtcagcactt gccactgtaa accgttggaa agcgtcccct
    14101 gttgccatgc tggatcaggc tggagcgcat ctgcctgcac atagccagca gtggccatca
    14161 tcagcaaacc gcccgctaaa agtcgaattt ttgtgccctg catgtgaacc cctgatcaac
    14221 tatcctggta ataaaaaaac tgcccgttgt cggtcagtct aaatgacgtt aaaaacactt
    14281 cgtgttagac cgcaagaaca tgaaaatgtc acggaagaag tgaaataaac ctgaactcac
    14341 ccaggtggta agttcaggca caggggtcaa ttatgcgcaa acacccgcac tcggggaagg
    14401 gagtgcgggc ataagtgatg agattaagag gataattcgt gcgttttgcc atccggcgca
    14461 cgattattca gcacatcgtc cagttttttg tggtccagtt ctttcaccca cttagcaacg
    14521 acaatggtcg ctacgccgtt accgaccagg ttagtcagcg cacgagcttc tgacataaag
    14581 cggtcgatac cgaggatcag cgccagaccc gctaccggca aatggcccac cgcagagagc
    14641 gtcgccgcca gcacgataaa gccactaccc gttacccctg ccgccccttt agaagaaagc
    14701 agcaacacga ttaacagcgt gatttggtgg acgatatcca tctgactgtt agtggcctgg
    14761 gcgataaaca ccgccgccat tgtcaggtat atcgatgtgc catcaaggtt aaacgagtag
    14821 cctgtcggga tgaccagccc caccaccgat ttacggcagc cgagtttctc catcttgtcg
    14881 agcatacgcg gcagcgccga ctcggaagat gaagtcccca gtacaatcag cagttcttca
    14941 cggatgtagc ggataaattt gaagatactg aaaccagtcg ctttagcgat tgaacccaat
    15001 accagcacca caaacaggat acaggtaatg tagaaacaga taatcagctg ccccagttgc
    15061 accagtgtgc cgacgccgta tttaccgatg gtaaacgcca ttgccccgaa cgcaccaata
    15121 ggcgccagac gcatgatcat attgatgatg ccgaagatga cctgcgagaa actttcgatg
    15181 acgttaaaaa tcagttggcc tttgctgccc agacggtgga gcgcaaaacc aaacagtacg
    15241 gcaaacagca gcacctgcag aatgttaccg ctggcaaatg cgccaatgac gctcgccggg
    15301 atgacatcca taatgaaggc gacaatgccc tggtctttcg cctgatcggc gtaaaccgct
    15361 accgctttcg catcaagcgt tgccggatcg acgttcattc cggcaccagg ctgcacgacg
    15421 ttaacgatga taagaccaat aatcagcgcg atggtactga caatttcaaa gtaaagcagt
    15481 gcgactgcgc cggtacgacc gaccgccttc atgctttcca tgcccgcaat gcccgttacg
    15541 acggtacaaa agatgacagg agcgatgatc atcttaatga gcttaacgaa gccgtcgcca
    15601 agcggtttca tttgctcgcc tatttcagga tagaaatggc caaggagaat accaatggct
    15661 atcgctgtca ggacctgaaa gtaaaggctt ttaaacagag aggttttcat agggtgtcct
    15721 ttagtaaaac cacaggtctt gtaaggttat ggggtacctg cggccttaaa ataacaccca
    15781 gacaacatca cagaaatgta cctggatcat aattgaaaca aaaaggttaa aaagtttgag
    15841 ctggctcgca caaaccagca ctttttaaag ttttgtaatc agtttggggt agctactttt
    15901 cttccaggta actctcttcg aagatttcaa tagggagtgg gcgagcaaaa aggaagccct
    15961 gggcaatacc aacgcccgct tttgccagcc agtcgcgttg tgcttcagtc tccacgcctt
    16021 cggcaatcat ttgtaagttc aggctctgcg ccagcatgat aattgcagca atcatgctgc
    16081 tatcttccgg caagccttca acaaacattt tgtcgatttt cagtacgtcg attggcaacg
    16141 atttcatatg ctgcagctga cgcagccctg cgtagcccat gccgaaatca tccagcgcca
    16201 cccgaactcc ggcattgcgc agcggacgga ggattgccac cgcagcatga gggtcgtcaa
    16261 tacgtcggct ttctgtcact tccagaatca gtgttcccgg ctgaatgcga tagcgggtta
    16321 acagttccag catatccgcc accatatttg ggtgcatcag ttgcagcgca gagaggttta
    16381 ccgacaaggg cagcataatg ccgcgctctt gccaggctgc aagcaatcga caggactctt
    16441 ccagcaccca gtgaccgacg gtaaccatca gcccacagca ctcaatgcga tcgattaagc
    16501 catccggcag gtcccaactg ccatccggtt gctggatacg cagtaacact tccgcactga
    16561 ccagtttacc gctggtcatc tcgacctgtg gctgtaacca aatagcaaac tgatgatttt
    16621 ccagtgcatt aaggatatcg ctctcttccg tcaaccgctt ctgggcggct tccatctgct
    16681 gcggatcaaa gaactgaatc tgattcttgc ctttatggcg agcggtaaat gccgcagaaa
    16741 tagcgcgact gtaaagctgt tcggcggtga gatcgccgta gaacatcgcc acgccaatgc
    16801 tacagtgcgg acggagttga atacgttcaa tcggcaggcg ctcgctcatg atagtgagca
    16861 cttgctgacc taaggtgatt gcgtgccacg gttcctgtac accgttggca atgacagcaa
    16921 agtcataacc gctaatctgc gcgaggatca tacgtggcga cagtaccgat ttgagttttt
    16981 ccaccagcgt cagcagcaga atttctcgtt gcgcctcttt cagcacgccc gcagtatcac
    17041 gcagggtttc acaggtgatg atcatcagcg cggtggtttg tttacgcgcg acaacctgct
    17101 ccagcatctc catcagcaag gctttgttcg gcaaatccga caccgggaag cgcatcgcat
    17161 tttcgttctg ttcttcataa tggcgctgca gcaattgctg gttgaggttg taactgcgca
    17221 ccaacatacc gatttcatcg tcctgatgca gacgcggtaa tgccagttgg tgaccaacaa
    17281 gctccttggc tgggatggcg ttaagttcgc gagcaatatt gcgtaacgga tgcaaaatca
    17341 ggcggttaat gcaccagctg atggcgacgg tcaggataag cgacaaaagt aagtaaatgg
    17401 tcactaacgt tgagagggtg ctcatcacga acttatacat acggaaggaa tccgcctgta
    17461 gtaccagata ggcaattggc tgcgggtttg ccggacgttc gagcgagtaa acgcccagcg
    17521 agatttgaac cggtagctca aacaggcgag taaccattac cggcaccggg cgctctggaa
    17581 taaaactttt acgcagcgcc tggaactggt taggcagcac tacatcggca cggctgacca
    17641 cgcctgccgg tttaatgctg gcaagaatag cttccgcttc gggaatatcg cctttcaaaa
    17701 tagctgaaga taagggttcg cggacagagc gagcaatgct ttccagttgc gtagccgtgt
    17761 tatagcgatt ctgctggacc agatggaaca gcaaaacggt gcaaaaaata aaaacgaaca
    17821 ccaggacaac ggctgccacc attgccatct gcttgattgt taacgagcga cttacgcgca
    17881 aattgtctcc ccccggaaat cccagattgc cgcccgagta tacccgatcg cagcggcatt
    17941 aagagaggcg ctatctgaaa acttaccagt cggcgtaagg tatcagcggc tgcggcggta
    18001 aatccatgtc accctgccat ccggcggcgg aataacgtac gtagagcagg aaatggctgg
    18061 gtgcgtaatc tttcgcctgc tggatatcaa ttgccgtgcc aacaaaccag ttggaagtaa
    18121 cacgtcgttc aagtaatgcc cgcgccgtgt agccgaagcc ctgactgctg ccgccatcgt
    18181 tggattgccg cgcagcttct tcctgccagt cggtcgggat cagattcatc agcggataac
    18241 gcggcatggt tttggtgcgt gaatgcgacc acgagccaga cgcacccagc tcccacgacc
    18301 agttttccgt gcgctcccgc cacatcaccg gtatggcaaa cgacaggtat tcctgcggac
    18361 tgtagtaacc gccctgaccg agtgagtagc cactcagatc tttgtcgtaa tgccagatca
    18421 tgttgttcag gccgattgtg acgcggcgat tgttctggtt aatgacctta tagtaatagc
    18481 ccgtcatcca gcgcacgcgc cagttatctt cgacattttt accggttaac tggtcgccac
    18541 taagcgatgc ccagacgccg tttgcttcac ctttatcgta gctcagactt agccccacac
    18601 cgtcggcacg tacgccaccc cattttttcc cggtattgct cggggagtct ttttgcccac
    18661 caaaggccag caaagaactg gagatgggcc gacggtgggc gttaacggtg taacccagcg
    18721 gcccgatatc atcgctgtaa ctgatgccgc cgaccacatc caccacgttg aagcccatcg
    18781 gcgtggtacc gatatcccag ctccagacgt catttcgcca gccgaccgcc acgctggcac
    18841 cggagtccga ctggctgcgg ttgccgctac agtcctgtaa tgtacaggtg ccccagttgt
    18901 catcccattt gccatcggca ttagtggaga aactgccgac gttcatattg acgaaatcac
    18961 tgcgaaagaa catccgcccg tcagaatacg gcgcatccac ctgcaacatg gtagtgtgcg
    19021 ctttcagatc ggagtaacca ccggtgccgc tcgaacccca gtaatcgtgc tcaagggtga
    19081 cgttaagatc ctgctggcga tagaggtccg ccgcatcgct gcgcacgcca cgtttcagcc
    19141 agtcatcttt ctcgtcgtta cgggtcagtc gggtaaaggt gtcgttatcc tgcggacgcg
    19201 tcgtagtcac accggatgcg accatggcgt ctttgtaggt ttccagcgcc tgcgttggat
    19261 cgcccgcctg cgcttcaaac ttcgcaccat cacgcagcac catcgcgctt tccatcgacg
    19321 gtggctgaga ttttgcctgc gggatcaact tattaaacgt ccgctgcgct gctgcggtat
    19381 cgccaagctg cgcctgtgcc agcgccacgc gccgctgtgt gttcagcgag gcgttatcgg
    19441 tagcgggcag tttcgccagc tggctacgtg ccgccgcttt gtcaccggca gcaatatcca
    19501 cttccgtcag accaagaatg gcgtcggcgt tagctggctc ccgcgtcagg acattctgat
    19561 atgcagcgcg ggcggcggtg taatcacgtc gttgttgcgc ccagtcagcc agcgtgaggt
    19621 caatacgcgt ggaaggtggt tgctggcgca gcatcgcttc cgcttctgcc tctttgccgc
    19681 tttctcgcag gcggttagcg gtttccagca cctgatcgct ttgcagtcga ttaaccagct
    19741 cctgaatatt gctgttccac tgcgcacgcg gcaggctgtt gatatgcgcc agcgccgctc
    19801 tgtcctggtc atgaccagag aggtacagcc cgtaagcgta aacctgctcc gggtcgttcg
    19861 acttctgctg cgccagattg cgcattaacg tatcggcctg gctgcgttgt ccggcctgcc
    19921 agagatcctg cgaaagtcgg taagtaatcc atacgctgcc ggggtccagc gccagtcgtt
    19981 gccgctgaag tgctgccgcc tgcgcccatt tgccctggtt ttccagtgcc tctgcctgct
    20041 gtgccagacg gtcgttttgc aggctgcgtt cgatatcatc aatgctacgc cgctgactgg
    20101 cagagagcga ggcgataaac gcttcagctt tttctggcga ttgctggcgg taaatatttg
    20161 ccagcccgcg cacggcgtta gtgttgccgc tgtccatacg caaggtctgc tgataataac
    20221 gttcggcggc gggataatct tttcgcgcca tcgccacatc gcccagcccc agcactgcat
    20281 aactgtcggt gttatcgaca ttacgcgcct gctggaacag gcgttctgcc cggtcaggat
    20341 tattggcttt cagcgcagca tcgctctgct ggatcgccag ccagtagcgg tttactttca
    20401 gcagactgtt ccatttgtcg ttgttgctgc tgtgcgggtc cagtgcgagg gctttttcca
    20461 gattcgccac tgcattggcg cgatcgcctt tctgagaata cgcctggccc agcgccccca
    20521 gagcttcact gtctttcggg ttcgcccgca ccgcctgttg tagttcggga atggctttac
    20581 ccgccatacc agagtccacc gccgctaaac cttgcgcacg agcgcggaaa gcaggatcgg
    20641 ccagctgttt ttgctgttct gccagttgcg attgcgcagc cgccacgcta tcgccatcac
    20701 taaagatcga gagatatttt ttcagcgccg acacactggc atcactgacg ggcatgtctt
    20761 taatctgccc gtaccagatt ttagaggccc cttcgcgccc ggcgttcgat tttgccatct
    20821 gttccaggac ggcaaaacct tcgtcacggc gatcgctact aaacagcaat agcgccagat
    20881 tgttttgcag gcccgtattg cccggtgcat ccgcattgat gcgttttaac tgattaatcg
    20941 cttcgccacg gcgagccgga attttcgcca ccgtactcca gtactcgaca gcaatgtcac
    21001 cttccggcgg cgcaccgttg aacagtttgt tgtaactcgc cacagcttct tctgcatgac
    21061 cggtcgtcgc ctgcaatcgt gcctgttgca gtgcctgacg accatccggc gtggaaagta
    21121 gcatcgtagt ccgcgacgat ttatacgcat ttgaactcgg cgctaactgc gacagccgat
    21181 cgagctgttt ttgcgcgcca tcaatatcgc cctgacgtaa caaagaacgg aaacgggcgg
    21241 caacgacgtc cgggttattc ggatcaataa gttccagccg atataacgac tgttgcacca
    21301 gatcttcacg atgggtcgct tcgcctaacc gaacttgctc cagcaactgt tgctgagcgg
    21361 ttggtgctgc ctcgaccatc ggcatgacgg ccagaccgag ggaaagcgtg aatatgttta
    21421 gtgtgaattt gcgcattcct ggccccagtc aggtaataac tcaccttttg tcgagaagcg
    21481 gaaacggtgt tgatcccagc cttgtccaaa cagggtcagc acatagttgt aataggcatc
    21541 gctgccggga aagttatcgg ccacgcgctg gcgctgaacg gcctgcgcat cgcggttttg
    21601 taaaaagggc agcatggcgg cagaaaaacc gactggtcct ttaccctgcg ctttccccgt
    21661 agccacatcc actttttccg gcggataacc gtttttctca gtgaatgtcg ccatcggttt
    21721 aaaccggttg agcatccgcg ctttttgcgg atcgctgtca ggcatcatgc ctacccacat
    21781 gtaaacgcgg atagcgtcgt agctgctgat caatgttttt tcggctttta gctgccagcc
    21841 tttgtctttc tcatagcgca cccagtctgg cgaaaaacct ttcggggcgg tttccagcaa
    21901 taaacgttga ttggtttcgc gcagcgtggt ccacggcgcg ccaaagcggg tgaaatactg
    21961 cgccagcgtc ggcggcaggt agctggggtt aaaacgccag ctgttatcct cagcaaaacc
    22021 cactttgccc ggtaacaaca tggaacccag cccaggcacc gtcaccactt cctcccgcgc
    22081 gatacgtttt agcaacgcgc tgccgatgtc ggtataacgc tgctctttcc acaaacgccc
    22141 cgcctccagc aacgaccagg ccatccagac atcaccatcg gaggccgaat tgctgtccag
    22201 cacttcccac ttactgttct ctttcttgcc ccacagccag gcgggcaaac gttcttttaa
    22261 agaaccctga gcgagattgt tctgcgtcca gtcgagaata ttatcgaaag ctgcacggtc
    22321 gttagccgcc agggcagaga acatgccgta actttgccct tcggaggtgg tgattttgcg
    22381 cgcgtcgctg gggtcgatga cgcgcccttc ctgactgatg taatcctttt taaactgctc
    22441 ccaggcaggc caggtacagg ctgcctgaac actaaaggca gccagcagca gcatcgtcac
    22501 gattccacta cgcaacacat tcatcttcaa ttactcgtta tccgggttaa gacgacgacg
    22561 actaataatt cgcagcagac gccacagtac ccatgccagc aatatcacac tgatagccgc
    22621 cagcaccgcc agcagaatcg gatggtttgc cagcgcatac cacacgcgct cgaaccacgg
    22681 cagatgacct acgtaataaa cgtcgccaac acgtaggctg ttgataccgg actcgcggat
    22741 caccgcgacc gaaccgaaca tggtggcgcg tttgccgcta tcgttcaccg catcgttaag
    22801 catttcataa ccgcgtgggc tatctgccaa cagcgcaatc acgctgcgct ggtcgttata
    22861 cggcgactgg aagccaatca ccgccgccat cgcaccggaa gaggtcagcg ttgaccgggt
    22921 ttctgccgcg cgatcgctct cgtccggcac aatgccgggg aacggggtct ggcgcatcgg
    22981 tgttttcacc cagctttcgg tcgcctgcac caataggtcg atctgcttat cgtctttcag
    23041 tttgtccggg ataccaccga tgatcatgat gtcggcatct ttgccctgaa tggtgctgcc
    23101 atcatcggtc accgtcagat taatcgccgg gaagcccgtc tgtgcgccga taaaaccaac
    23161 agtattcagc aacgtttcca tctgtgcttc gttaggcgct ttcggcatca cggtgatggt
    23221 ttgcgacaga tccgccatcc ggctgaatgg gaagcccgcg ttagcaaagg cgcgtagatc
    23281 cggcatcggg atgaagtggt aatacttcga gaagtcgatg gtggagtcgt caccaatcac
    23341 cacatgattc tgcaccggct ggaaggtaat acagttatcc accgaaccgc ccggcatcgg
    23401 gttcatatac tcaaagtcga agcgcagctg gttggtcgcg cccagtttca gcgccggaat
    23461 agagacatct gttttgccat ccagcaaacc ttgtaatacc ggaatccgca gcagcaggcg
    23521 gttcgcctcc tgtttgctgc tcaggttgaa ggattgcagg aactggttat tcaggctgat
    23581 atccatccgc gaactgtctt tcaccggcgg catggtgtag cggtaattaa tatccatatc
    23641 aatgccggta ctgcgcatca ggtagagatc cggcggcagg tttagcgaaa cgttaatcgc
    23701 tgctggctca agaccgctgg attgtaactg ttcttcatag gttttcagtt cgccaaatgt
    23761 gaccggacga tcggtacgta cccagttcgg cgcatcgtac ggcttacgcg gtagcagcgg
    23821 tttcacttca ttcactacca cgctttcacc acggaacaga atgttaccct gagcgatacc
    23881 tttcgctgcc tgcaacaggt ctttgtcgtc acgaccaaac accaccagca gtttgacgta
    23941 aggattctgc ggatggttaa tcatctcaat caccggggct tttaccgccg gatgatcgcg
    24001 caggaagtcc ggccgtttgt cgttggttgc aaagacaatg gcattacgat ccggcagttg
    24061 gttatagagc accgggaagt tctgcccacg ccagccagaa cgcgaaccaa accacgaggc
    24121 gacaatggca gaggcttgtt gcagcccaac atccggcgca cccgcaaaga ccatcggcaa
    24181 ggtgttagta cggttatcgc gcgggtcaaa gaatggcacc gggaagtgtg acaggtcatt
    24241 cttcacattc agggtctgat aggtcagatc cagtccactg ctccgcccaa catccagcca
    24301 aagcgtggtg ctggccgggt tttcgcacac gtcctgataa tggccgacaa actccagccg
    24361 tacacggttg aagtcggtaa taaacagtgg gttaatgggc atttgcgcca gcgttttttt
    24421 acccaactgt tctttggtca ctggcagcac gcccatcagt tcatcattga gataaacctt
    24481 taactgcgac tggacaggca gtaacgatgg cgatggggtg tattcgaggt tgagcatcgc
    24541 cttcgtcacc acttcatcgc tgcgcatacc aaactcaatg ctgccgttcg ggttaatgcc
    24601 acgtagcacc atgctgcccg gcggcggtgc aatttgcgca aaggtcagct tcacatcacg
    24661 cgaaggaccg ttctgcgcca cgactggcgc atcagcgccc tgcacgcccg gcatcacttg
    24721 ccccacctgc ggattttgtt ccgtctgggc ggctacagct ggctcagcat tgatcagtgg
    24781 ttgcgttgct ggcgtcgcct gcgtcatgaa agaggggaac gcactcatcc ccatagccac
    24841 tgcacaaatc cagaatagtt ttcttttcat cgcgttatca tcattgttga gccaaagcct
    24901 gatccgatgg ttgtgccgtt tcgctccgct ccgggcggcg cggaataaac gatacaaccc
    24961 aggaaaccag agaagtcagc acacggaata tgcccttcac cgaagaaggc gcaaactccg
    25021 ccagatggcg gtagccacgg aagccgagct tcagaatatc cagcagactt tccagcggct
    25081 tatcttccgg gtagctgtcc tgccagagcg cccatgtatc cgcacgggca aacgtacact
    25141 gcacaaaatc gatatgttgc tgggtggtga gcggcattaa tttcagccca acttcattac
    25201 ccatcacgcg cgccacctgg gtcgggaaga cgtattcctg ctgaccgcgt ttaagcaaca
    25261 gattcacttt ctgcccttcc agaatctgcg cctgaccgtt gatcttgatc cccaaaccac
    25321 cgtcggagaa atcctgaacg gtacacgaga agaggtgacc atcttcgcgg gcaattgccg
    25381 cgggcatcgt catctccacg cggtgcgatc ggcgtacctg tttgctttct accgataccg
    25441 caactgcgcc gccaagaaca atcaggttgt agaacaccca caccatactg acgaccacgg
    25501 tgagcatctc ggttggcggg ccatagaagt agcgccagat gcctaccgca acgcccacca
    25561 ggttgagcag gacaaggaag atgtagggcc gcgagatcac ccagtcgacg tactcttctt
    25621 ccaccagtcc acctttggcg gtgacgttaa atttgccttt gtgcgggtta atcagcgcca
    25681 ccagcgtcgg tggtgcgata taccacgcca gcaccgtttc gtagatttca ctccagaaag
    25741 agtggcgata tttgccctgg atcttggagt tggtcaggct ggcatggatc atatgcggca
    25801 gcacgaatag gacgatcatc aacgctggcg catagatgat gtaggcatga agcagcagga
    25861 acgccagcgg cgcagtcagg aagatcagcc gtggaatgcc cgacaagaag tggaacatgg
    25921 cgttgacgta acatagccgc tgagcaaact tcagcccttt accggtgagc gggttatcga
    25981 gacggaagat ttgtaccatc ccgcgcgccc agcgaatacg ctgaccgata tgcgccgaca
    26041 gactttcggt cgccagcccc gccgcctgcg gaatacgcat atacgcggag gtatagccac
    26101 gacggtgcaa ccgcagagaa gtatgcgcat cttcagtcac ggtttcgaca gcaatgccgc
    26161 caatttcatc cagcggctta cgacgaatca ccgcacagga accgcagaag aaagtggcgt
    26221 cccacatatc gttgccatcc tgcaccagac catagaacag cgtgccttcg ttcggcgttt
    26281 tacggaaacg ccccaggttg cgttcaaacg ggtccggtga gaagaagtgg tgaggcgtct
    26341 gcatcatcgc cagctgtttt tctttcagga accagcccat ggtcatttgc aagaacgatc
    26401 gcgttggtac gtggtcgcag tcgaaaatcg acacgaactc gcctttggca tatttcagcg
    26461 cattgttgat gttgcctgct ttcgcatgtt catgagtggt gcgggcgata tatttcaccc
    26521 ccacgttttg cgcaaactgg cgaaactctt ccctgccgcc gtcatcaagg atccagatat
    26581 tcagcttatc tttcggccag tcgataccca gcgaggcgta aatggtattt ttcaccacgt
    26641 tgagatcttc gttgtaagtc gggacaaaga tatccaccga cggccacagc gacatatctt
    26701 tcggcaatgg caccggctga cgattcagcg gccatactac ctggaagtag ccgagcacca
    26761 acacaatcca cgcgtacgtt tcagcgaaga gcagaataag cccgcacacc aggctgaccg
    26821 gatcgtccca gttcagcgta gaggtgtaac gccaccagat ataacggcaa gaaacggtca
    26881 gcgacagcac aatcaacatt agcgccgaga agcgccccgg catccgccgt acgatcagcg
    26941 ctacccccca aagcagcatc aggaagataa actgcgccag cgggttaaac ggctgagtaa
    27001 cgcagattaa cgccagaatc agcgagaagg tgacgatgat accgaggatc aaccgccgcg
    27061 ccccggcact caaatgaccg agctcttttt tctcatcaag atgctgtgtt ttatggctaa
    27121 cgcgctcagg cagctcgttc atccattgat ggtaacgtcc acgaatattt tgcagacctg
    27181 aaaatgcccg cctgcgcggt ttcggcgttt ctttgcgcga tgcaccgatc aataaccagc
    27241 atgtttgaat gagataacgg accgggtcca gcggacgcgg acgcgaggcg ttgatatgcg
    27301 gatacaggtt tttatgttct gcgcgaatac gctgccagcg cgggtgctcc agcggaataa
    27361 aaatccaggc caggatcatc cagaaacagc cgagcgtcgc gctgaaagcc gacgcaccgt
    27421 ggcgacgata atcgcgataa cgcccgatca gccgcgcgtt gaccggcggg attagcaacc
    27481 accgggtcag gatactcatg atttactccc gactggcgtt ttcagcccgg aatagttcaa
    27541 caggcaccag ttcgccagcg tcagtatctc ttcagccgcc agcgcatcac tgcgatattc
    27601 acctactggt tgcttagccg ccaggcattc agccatcgct tcatcacgat gaatgagcat
    27661 cggcagtaat cggcgctggc tttgcaacca aagctggtaa atatcgtcct gaacctgact
    27721 gccaatacgg aagttattaa tcaaaatatg tgcgccatcc ggcagcgctt gctgatgcag
    27781 tcggatatgg cagttggcat ccacattgac gattgccagc gagtgatcgc acaaactcag
    27841 cagctggtgg gttatctgcg aggcatcacg cggtaagtcg attaaaatcc actggtaacg
    27901 cccgctggct gtaagccgga gcaaatatcg ctcagccggg tttgccagtg ctgtggattt
    27961 tcttgttctt caatggataa ctgaccaaaa ggcagcaaat cgagctgcga ggtgtagcgc
    28021 aacccagcgt cacgccagtc ctggccatcc agcatcgctc tggcccagcc ctgacggtgg
    28081 gtaaaatcaa cattaaatga caggcgcaac aagttgtccg ggcaggcatc gaccaccagg
    28141 acattttctc ccaacatttg taatgaccag gctaatgcgg cggtgatggt tgttgtcccc
    28201 acgcctcccc gcaccccctg caatcccagt acggccattc accaatcccc tacttttgtt
    28261 gcgcaaactc tgccagcaac ggccagcgtt ttaatgccgc ggccaactgt tcgcgttggg
    28321 aaatatcggc ataatcaata tcaggcagtg aaaatgcctg ctttaacgcc acaatatcat
    28381 tctggaagat atagcctatc gcgggatcag gcagagtatc tggttcattg ttattcattt
    28441 tgcttgatcc ctatgaaata accgtaccgt tcatgagaca attctaaccg tgagcatttc
    28501 gccggtttat tgtttacggc gagacgggaa tttgctcact ttttgacttt ccgttacgca
    28561 acagaaaaag ttaaaaacga aaagactaaa atcacacgtc ttgtgacctt ctaaattgat
    28621 tcacatgcta aatctgataa gttttaattt caatggtagg tttatttctt agctttcgct
    28681 agtaaactga taatcagaca aaatggtgac atgagggaca ttgtggaccc tgtattctct
    28741 atcggtatct catcattatg ggatgagctg cgacatatgc cagcaggcgg cgtctggtgg
    28801 tttaacgtcg atcgccatga agatgctatc agtctggcga atcaaacaat tgcatcccag
    28861 gctgaaaccg cacacgtcgc ggtcattagc atggacagcg atccggcgaa aatctttcaa
    28921 ttagatgatt ctcaagggcc ggaaaaaata aaattatttt caatgctaaa tcatgaaaaa
    28981 ggtctatact atttgacccg tgatttgcag tgttctattg atccccataa ttaccttttt
    29041 attcttgttt gcgcaaataa cgcatggcaa aacattcctg ccgagcggct tcgctcatgg
    29101 ttggataaaa tgaataaatg gagcaggtta aaccattgtt cgcttttggt aattaatccc
    29161 ggaaataata acgataaaca attttcattg ttgcttgagg aataccgttc actttttggt
    29221 cttgccagtt tgcgttttca gggtgaccaa catttgctgg atattgcctt ctggtgcaac
    29281 gaaaaagggg tcagcgcccg tcagcagctt agcgttcagc aacaaaatgg tatctggaca
    29341 ttagttcaaa gcgaagaggc ggagatccaa ccacgcagcg acgaaaaacg cattctgagt
    29401 aatgttgctg tactggaagg tgcgccgccg ctatcggaac actggcaact gttcaacaat
    29461 aacgaagtcc tgttcaatga agcccgtacc gctcaggcgg cgacggtggt cttttcttta
    29521 cagcaaaatg cgcaaatcga gccactggcc cgcagcattc ataccctgcg tcgccagcgc
    29581 ggtagtgcga tgaaaatcct cgtgcgggaa aataccgcta gcctgcgcgc caccgatgaa
    29641 cgtttgttat tggcctgcgg tgcaaatatg gttatcccgt ggaatgcgcc actctcccgt
    29701 tgtctgacga tgatcgaaag cgtgcaaggg cagaaattta gtcgctatgt gccggaagat
    29761 atcactacct tgctgtcaat gacccagccg ctcaaactgc gtggtttcca gaagtgggat
    29821 gtgttctgta atgccgtcaa caacatgatg aataaccctc tattacctgc ccacggtaaa
    29881 ggcgttctgg ttgccctacg tccggtaccg ggtatccgcg ttgaacaagc cctgacactg
    29941 tgtcgcccta atcgcaccgg cgatatcatg accattggcg gtaatcggct ggtgctgttt
    30001 ctctcattct gtcggattaa cgatctggat accgcgttga atcatatttt cccattgcct
    30061 actggcgaca ttttctcaaa ccgtatggtc tggtttgaag atgatcaaat cagtgccgag
    30121 ctggtgcaga tgcgcctgct tgccccagaa caatggggca tgccgctgcc tttaacgcaa
    30181 agttctaaac cggtcatcaa tgccgagcac aatggtcgcc actggcgacg aataccagaa
    30241 cccatgcgac tgttagatga tgctgtggag cgctcatcat gatgaccatc agcgatatca
    30301 ttgaaattat tgtcgtttgc gcactgatat ttttcccgct gggctatctg gcgcggcact
    30361 ctttgcgacg cattcgcgac accttacgtt tgttctttgc taaacctcgt tatgttaaac
    30421 cggccgggac gttacgccgc acggaaaaag ccagggcaac caaaaaatga ctcaatttac
    30481 gcaaaatacc gccatgcctt cttccctctg gcaatactgg cgcggccttt ccggctggaa
    30541 cttctatttt ctggttaagt tcggcctgtt gtgggcggga tatcttaact tccatccgct
    30601 cctcaatttg gtgtttgccg cgtttctgct gatgcccatt ccgcgctaca gcctgcatcg
    30661 cttgcgccac tggattgccc tgccgatcgg ctttgctttg ttctggcatg acacctggtt
    30721 gcctggcccg gaaagcataa tgagccaggg ttcgcaggtg gcggggttca gtaccgatta
    30781 tttaatcgac cttgtcacac gctttattaa ctggcagatg attggggcca tttttgtttt
    30841 attagtggcc tggttattcc tgtcacaatg gattcgcatt accgtttttg tggttgccat
    30901 tctgctatgg ctgaacatac ttaccctggc gggaccaagt ttctccttgt ggccagccgg
    30961 acaaccgacg accactgtaa caacgacggg tggtaacgca gcggcaaccg ttgcggcgac
    31021 gggtggcgca ccggtagtgg gtgatatgcc cgcacaaact gcaccgccaa caacggcgaa
    31081 ccttaacgcc tggctgaata atttctataa cgcggaggcg aaacgtaaat cgaccttccc
    31141 gtcttcgctg cccgctgatg ctcagccatt tgaactactg gtgattaaca tctgttcgct
    31201 ttcctggtcg gatatagaag ccgccgggtt gatatcgcat ccactgtggt cgcatttcga
    31261 tattgagttc aagaacttta actccgccac ctcctacagt ggcccggcgg cgatccgttt
    31321 actgcgcgcc agctgcgggc agacttcgca cactaatctg tatcaaccgg caaataacga
    31381 ctgctatctg tttgataacc tttcgaaact gggctttacc cagcacctga tgatgggaca
    31441 taacggccag ttcggcggtt ttttgaaaga agttcgcgaa aatggcggca tgcagactga
    31501 attgatggat caaacaaatc tgccggttat tttgctgggc tttgatggtt cgccggttta
    31561 tgacgatacc gccgtgctta accgctggct ggacgttacc gaaaaagata aaaacagccg
    31621 tagtgccacg ttctacaaca cgcttccact gcatgacggc aaccattatc cgggggtcag
    31681 caaaacagcg gattacaaag cgcgggcgca gaaattcttt gatgaactgg acgccttctt
    31741 tactgaactg gagaaatcgg gtcgtaaagt gatggtggtc gtggtgccgg aacacggcgg
    31801 cgcgctgaag ggcgacagaa tgcaggtatc tggcctacgt gatatcccta gcccgtctat
    31861 cacagacgtc cccgttgggg tgaaattctt cggcatgaag gcaccacatc agggggcacc
    31921 gattgtcatc gaccaaccga gcagcttcct ggctatctcc gatctggtgg ttcgcgttct
    31981 tgatggcaag attttcaccg aagacaatgt tgactggaaa aaactcacca gtgggttgcc
    32041 acaaacagca ccggtctccg agaactcaaa tgcagtagtt attcaatacc aggataaacc
    32101 gtacgttcgc ctgaacggcg gcgactgggt gccttacccg cagtaagcac taaaaaggcc
    32161 gcagagttta acctctgcgg cctgatccgg gcgcaaattg ccattacggc agcctgacgc
    32221 ccgcatgaca cgttacttcc gcttattcag ccagttcacg atcata
//
LOCUS       DAOFQD010000051        32226 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.051, whole genome shotgun sequence.
ACCESSION   DAOFQD010000051 DAOFQD010000000
VERSION     DAOFQD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.051"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(635..1576)
                     /gene="fabY"
                     /locus_tag="QBF46_002337"
                     /gene_synonym="yiiD"
     CDS             complement(635..1576)
                     /gene="fabY"
                     /locus_tag="QBF46_002337"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDQ6674512.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1621..2058)
                     /gene="dtd"
                     /locus_tag="QBF46_002338"
     CDS             complement(1621..2058)
                     /gene="dtd"
                     /locus_tag="QBF46_002338"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDQ6674513.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2055..2927)
                     /locus_tag="QBF46_002339"
     CDS             complement(2055..2927)
                     /locus_tag="QBF46_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDQ6674514.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2921..3520)
                     /gene="yihX"
                     /locus_tag="QBF46_002340"
     CDS             complement(2921..3520)
                     /gene="yihX"
                     /locus_tag="QBF46_002340"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDQ6674515.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3619..4404)
                     /locus_tag="QBF46_002341"
     CDS             complement(3619..4404)
                     /locus_tag="QBF46_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6674516.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4438..5334)
                     /gene="yihV"
                     /locus_tag="QBF46_002342"
     CDS             complement(4438..5334)
                     /gene="yihV"
                     /locus_tag="QBF46_002342"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HDQ6674517.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5502..6398
                     /gene="yihU"
                     /locus_tag="QBF46_002343"
     CDS             5502..6398
                     /gene="yihU"
                     /locus_tag="QBF46_002343"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDQ6674518.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6422..7300
                     /gene="yihT"
                     /locus_tag="QBF46_002344"
     CDS             6422..7300
                     /gene="yihT"
                     /locus_tag="QBF46_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HDQ6674519.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7317..8558
                     /gene="yihS"
                     /locus_tag="QBF46_002345"
     CDS             7317..8558
                     /gene="yihS"
                     /locus_tag="QBF46_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HDQ6674520.1"
                     /translation="MKWFNTLSHNRWLEQETDCIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8696..9598
                     /locus_tag="QBF46_002346"
     CDS             8696..9598
                     /locus_tag="QBF46_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HDQ6674521.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQLWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLESGKPHRLFFNIYGQRK"
     gene            9797..11833
                     /gene="yihQ"
                     /locus_tag="QBF46_002347"
     CDS             9797..11833
                     /gene="yihQ"
                     /locus_tag="QBF46_002347"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HDQ6674522.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            11879..13264
                     /locus_tag="QBF46_002348"
     CDS             11879..13264
                     /locus_tag="QBF46_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6674523.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPASVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            13307..14710
                     /locus_tag="QBF46_002349"
     CDS             13307..14710
                     /locus_tag="QBF46_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6674524.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILIGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            14778..15470
                     /gene="ompL"
                     /locus_tag="QBF46_002350"
     CDS             14778..15470
                     /gene="ompL"
                     /locus_tag="QBF46_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HDQ6674525.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYNFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15561..16826)
                     /locus_tag="QBF46_002351"
     CDS             complement(15561..16826)
                     /locus_tag="QBF46_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6674526.1"
                     /translation="MLTKKKWALFSLLRLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFSGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(16928..17908)
                     /locus_tag="QBF46_002352"
     CDS             complement(16928..17908)
                     /locus_tag="QBF46_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDQ6674527.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(17916..18626)
                     /locus_tag="QBF46_002353"
     CDS             complement(17916..18626)
                     /locus_tag="QBF46_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDQ6674528.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(18843..20666)
                     /gene="typA"
                     /locus_tag="QBF46_002354"
     CDS             complement(18843..20666)
                     /gene="typA"
                     /locus_tag="QBF46_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDQ6674529.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            21039..22448
                     /gene="glnA"
                     /locus_tag="QBF46_002355"
     CDS             21039..22448
                     /gene="glnA"
                     /locus_tag="QBF46_002355"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDQ6674530.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22623..23672
                     /gene="glnL"
                     /locus_tag="QBF46_002356"
     CDS             22623..23672
                     /gene="glnL"
                     /locus_tag="QBF46_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDQ6674531.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23684..25093
                     /gene="glnG"
                     /locus_tag="QBF46_002357"
     CDS             23684..25093
                     /gene="glnG"
                     /locus_tag="QBF46_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDQ6674532.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25205..25315
                     /locus_tag="QBF46_002358"
     CDS             25205..25315
                     /locus_tag="QBF46_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDQ6674533.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25505..26878)
                     /gene="hemN"
                     /locus_tag="QBF46_002359"
     CDS             complement(25505..26878)
                     /gene="hemN"
                     /locus_tag="QBF46_002359"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDQ6674534.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(27067..27576)
                     /gene="yihI"
                     /locus_tag="QBF46_002360"
     CDS             complement(27067..27576)
                     /gene="yihI"
                     /locus_tag="QBF46_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDQ6674535.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            28158..28790
                     /locus_tag="QBF46_002361"
     CDS             28158..28790
                     /locus_tag="QBF46_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDQ6674536.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(29172..31958)
                     /gene="polA"
                     /locus_tag="QBF46_002362"
     CDS             complement(29172..31958)
                     /gene="polA"
                     /locus_tag="QBF46_002362"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDQ6674537.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7864 a         8286 c         7944 g         8132 t
ORIGIN      
        1 ttatttgcat aatctgaatg gtgtagccga aaaacgtcag catcctaaca gcaccaggat
       61 ttaaggtgaa attaatcttt catcacagac cggtcagtac tgccaccaac cggtctttac
      121 ttgctcatcg ttataatttt gttggtaaca gttcgggaaa gttgtttttc taaattaaca
      181 acagaataac tttcttgttt aatgagatta atattggcaa ccattatagc ctccttccca
      241 cggtttaaga gcaaacatac aacataatta ttcataacat acagagcagt aaatgaatga
      301 tactgacaat gatgctagtc cattatcttt gcataatatt tgttagctat agattttaaa
      361 aatatttatg agaaaagtat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac
      421 atgaactaag atgatgaatt aagcattatc tgcgataatt gccactcagt tctacaaata
      481 atatttaaat gctcctgaac agtgatgtag caagacacgt atggagccaa cgaagaataa
      541 aaatttatag agttcttatt taatataacc gaaatagcca aggactaata taaatgacct
      601 gaatgagaga ggcagcaccg cccctctcca tcagctactc ttcttcgttc ccgccctctt
      661 catacgggcc aaatggcttc gcgggcagaa cgatatacgt gccttcaaac actgcacccg
      721 gcgtctcgtc gccaaagatt tcgacctgca tctgcacccg tgcttttcgt ccgcgcgcca
      781 gacggtcgag atcgccgctt aaggcaccga ggtcggctac cgcatgaggt ttaccgctaa
      841 tcggcttgct gtagcggata tgcgcatccg caagaataat cgttccgccg aggtggcgtt
      901 cacgcagcat cagccagata agtccccaac cggtgagcgt cgccagtgag aataaactcc
      961 cggcaaacag cgtatggtgc ggattctgat tgccggtttc tggcatggta gtgataaatt
     1021 tttgcccggt atattgctga atgcgcacgc ccattttttc actaagcggg atatgttcgt
     1081 accacgcctg ttgcagctgc gcgcaccagt cgccgcgatg cagaatgtca tccagagtgg
     1141 cgacgggctt aatcatcaaa aaatggcgaa tcggcgtggt ggttggcgtg gtgatttctc
     1201 cctgattaac aaaccccagc ttggcgaaaa actccaccgc gtcttcacgg gcgctacagg
     1261 tcacgcgctt aacgccttcc tgacgcgcca ccgactccag ggtcatcgcc atcagcgtgc
     1321 ctaacccttt gtcctgcacg tcgggatgaa cggccataaa gcgaatggac gcttcattgt
     1381 cggcattaat atacagtcgg cctaccgcca ccagattacc ctgctcgtcg acgaccatct
     1441 ggtgatgcgc catcgcatcc cacgcgtcgc gttccgaacc ttttggttga tgcaggggct
     1501 tacgcaacat ttcccagcga aactgatagt aacgctctaa ttcttcttct gtttgtggaa
     1561 cccgaaggtg atacatagcg atactctctc ttgtttcccg tgacaaccct ggaagctggc
     1621 tcatacctgc aaccagaatg tcacggggcc atcattgacc agcgatacct gcatatccgc
     1681 agcgaagcgt cctgtttgcg tgttcatctc ttgctgacgg cagcgttcga cgaaatagtc
     1741 atataacgcc tctgcgcgat ccggtgatgc acctttggag aaacttgggc gcatcccccg
     1801 ttcggtatct gcggcgaggg taaactggga aaccaccagc acactgccgc ccgcctgttg
     1861 cacgttgaga ttcatcttgc cttcggcatc gctaaagatg cggtagccga gcacacgctc
     1921 gcacagacgg tttgctttct gttcgtcgtc atccttttcg acacccaata acaccaaaag
     1981 tcccgcgcca atttcgcccg tcacttctcc ctccacggtg acgctggcac gggttacgcg
     2041 ttgaattaat gcaatcatgg ttcgtcgtct tcttcttgtt cagctgcttg ttttagtttg
     2101 cggtattccc cgagagtgac agtaatttcc gcgccaagca agacgataca ccatgtccag
     2161 tagacccaaa caaagagaat ggggatcacc gccagcacac cgtaaatgag ctgatatgac
     2221 gggaacatgg tgatataaag cgcgaaacct ttctttcctg cttcgaacag gagtgcggcg
     2281 acaaacgcgc cgacaatcgc gtcgcggtta ggtacgcgga tggtaggaac aatgctgtac
     2341 agcaaccaga aggagatcca cgacaacagc aacggaaaaa tacgcagcac gttgtcgatg
     2401 acagtattga gatcgctcgc ccagcgcagg gagagcaaat aggaactgat cgccagactg
     2461 gcccctgcca gcagcggccc cagcgttaaa atcatccagt acacggcgaa cgagtaaatt
     2521 tttggtcgcg ctcgtttact gcgccagatg gtattcaacg cgctatcgat ggagtacatc
     2581 aacaataacg cggtgacgat cagcccgcac gccccaacgg cggtcatctt gttggaattg
     2641 gcaacaaatt gttcgatata ccgctgaata acatcgccag tagcaggcag aaagttggca
     2701 aaaataaagt gacgtaactg aatgctgacg tcggaaaaca tgggaaaagc ggcgaaaagc
     2761 gcaaaaacaa cggcaaccag cggcactaat gagagcaacg acacataggc aaggttacct
     2821 gccagggttg tcatgttgtc ctcatcaatg cgttgccaga gtagttttag ccaggcccat
     2881 agtggacggg tacggtgcct ggctttgtcc tgaatggttt ttagcataac accttcgcga
     2941 aatagtccgg gatggtggtt ttatctttca ccagaatact ggtaatgccc agctgattgg
     3001 ctccttctat attatcggcg ttatcgtcga aaaagaccgt atcgctgggt gaaaaacctt
     3061 ccgcctgcaa aacatgctgg taaattcgtg cttcaggttt gcgcatcccc agatcttgcg
     3121 acagatagat atggtcagca gcatcacgaa tttccgggta ttcttccggc cagaaggtgg
     3181 tatgcaggcg gttggtattg gaaagcacca ccacgcgatg cccctgctca cgcagtttat
     3241 gcatgatggc gatcacttcc gggcgcagcg caacaaacac cgcctgccag ccgtgagaga
     3301 actgctcgta gcttagcggt agagccatct catgacacag cgcctctgcg aacgcttcgt
     3361 cgctaatttc cccacgctca tgctgatgaa acgcctcccc catatgaaaa ctcttcttaa
     3421 gcgatgccag cggaatacgc gttaaatcgc tccaggctcc cagcacacgg ttaaagtcga
     3481 tatcgacaat cacattacct aaatcaaaga tatagagcat ttttgcctcc tttcgcgcca
     3541 caagaaatta actgtagcgg gaaaggatgg gtttggctat gtggcaaggt atgaagccag
     3601 gttaacggtc acaaaaatct acgcgtcttc ctggggaaga atgatttcaa tttggctgtc
     3661 ttgtaagcgt gattttagcg cagcgggcgg cagctcgtcg gtaaagagcg cagtgacctg
     3721 tgccacgtta ccaatttcaa ccgccgccga agcatgatac ttagtgtgat cggcgaccag
     3781 cagaatattt ctcgcgtgcg ccatcatcgt tttcaccacg ttagcttcgt ttacatcaaa
     3841 ctccatcaac gcgccatcgc tctcaatcgc cccaacgctt gttaccagat aatcagcacg
     3901 aaaatcagcc acaaaggacg ccgctgaagg gccaatgatc ccgctattat gagagcgcaa
     3961 cgtaccgccg ggcaccatca cttcaaagcg cgggttgtgg taaagaatat gcgccacacg
     4021 caggctgttg gtgattatcc gcaaatgatt atggttaagt aacgcccggg caacatgctc
     4081 aacagtcgta ccaatggtga taaatattgt tgaaccatca ggaatatagt ctgccaccgc
     4141 ttcggcaatc gctttttttt cctcggtttg cgaaacctca cgctgctcga acgccgtatt
     4201 aacgacgctg gaagcccgac ccgcgccacc gtgatggcgc gtaatcaggc cttgctcgct
     4261 taatttacga atatcccggc ggaccgtctg cgtggagaca tccagcagat ttgccagctc
     4321 atcaatattc atatacccac gctcggcgat cagcatgagg agttggtcgt gccgcgggtt
     4381 accggttagt tcggtaaggc tcatgaaaat tcctcgaaaa accatcaccc tggcatttta
     4441 tacaaaaagt gacaaaaaag atcgggtttg atcacagtca gggatcccgg ctcgtccacc
     4501 gggacgtgtg cattttaacg ccgctacacc gctggcgaag cggactgact ccgctaaatc
     4561 cccacttgtt gccagcgcca ccgccaaagc gccgtgaaaa acatcacccg cacctgtggt
     4621 atctaccaca tcaactttga aggccggttg atgctgacgc ccaccatttt ccagccagtc
     4681 gcagcctgcg ctaccctggg tcacatagac atgaccattt gtgagcgttt gtgcctgttt
     4741 tagcgcactg gccatctctt tcacgcccgt taagcgcgcc agacccggtt ctgaaaaggc
     4801 cgcgtgatcg cttaatgcca ccagctcact gatatcctgc ggcgtaatgt ccccgtccag
     4861 aacggtcatc acacccgcct gacgggccag ggtgaaggct tttttagcgc cgtcgtgcca
     4921 gcgtacatct gccagcacaa catcccactg agagaaatca atttcctcca accactctgc
     4981 gtcaggcagc agatccgggc tggggtagtt aatgattatc cgctcgcctt tggtatccac
     5041 catgatggcg gattgcgaag atttcgcctg gttataccgt ttggtgtaac gggtgttaac
     5101 gccccaggat tccagctctg ccagcaggct gttgcccgtg tcgtcatcac ctacgcgacc
     5161 aataaaatcg acctgcgccc ccagccttgc cgccgcaacc gctgccgtcg ccgctggccc
     5221 gccaccaact tccgtataat ttctcgccac gtatttaccg ctctccgtcg gtaacccttc
     5281 cacgtaatag atgcgatcca tcacggttat acctacacaa gcaacacgaa tcatggtcat
     5341 tccttaaaca ttttgggatg cgttcatttt aatttcacca attgtttaaa aagtgacggc
     5401 tgtcaatttt ttgacgataa attacaaata cgatcaaaaa cagacaagca aagcatgtca
     5461 taaatgacaa aaagtgacat cactgtattc aggagagggt tatggcagca atcgcgttta
     5521 tcggtttagg acaaatgggt tcgccaatgg cgagcaattt attgcagcaa gggcatcaac
     5581 ttcgcgtctt tgatgtgaac gccgaagctg tgcggcatct ggtagacaaa ggtgcgactc
     5641 ccgccgccaa cccggcgcag gccgctaaag atgccgaatt tatcattacc atgttgccga
     5701 atggcgatct ggtgcgcaac gtgttgttcg gtgaaaacgg cgtttgcgaa ggcttatcta
     5761 ccgatgcgct ggtcattgat atgtccacca tccatccgct gcaaaccgat aaattgattg
     5821 ccgatatgca agccaaaggc ttcagcatga tggatgttcc ggtaggccgt acttctgcaa
     5881 atgccattac cggtactctg ttactgctgg ctggcggcac cgctgaacaa gttgaacgtg
     5941 ccacaccgat cctgatggcg atgggcagtg agttgatcaa cgctggcggt ccgggcatgg
     6001 ggatccgcgt taagctcatc aacaactaca tgagcatcgc gctcaatgcg ctttcggcag
     6061 aagccgccgt tttgtgcgaa gccctgaatc ttcccttcga tgttgccgtc aaagtgatga
     6121 gcggtaccgc cgccggtaaa ggtcacttca ccacttcctg gccaaacaaa gtcctcagcg
     6181 gagatctttc tcccgccttc atgatcgatc ttgcccataa ggatcttggc atcgcccttg
     6241 atgtcgccaa ccagctgcat gtgccaatgc cgctgggggc cgcctcacgg gaggtttata
     6301 gccaggcgcg cgcagcgggt cgcggtcgcc aggactggtc cgccattctg gaacaggtcc
     6361 gtgtcagtgc cgggatgact gccaaagtaa aaatgtaacg actggataaa ggaataaatg
     6421 aatgaataag tacaccatca acgacattac gcgcgcatcg ggcggttttg ccatgctggc
     6481 ggtcgatcag cgcgaagcca tgcgcatgat gtttgccgcg gctggggcac ccgctccggt
     6541 agccgatagc gttttaactg atttcaaagt taacgctgca aaggccctct cgccttatgc
     6601 ctcggcgatt ctggtagatc aacaattctg ctaccgccag gtggttgagc aaaacgcgat
     6661 tgccaaaagt tgcgccatga ttgtcgccgc cgatgagttc attcctggca acggtattcc
     6721 ggtcgatagc gtggttattg accgcaaaat caatccgcta cagatcaaac aagacggtgg
     6781 caaagcctta aaactgctgg tgctgtggcg tagtgatgaa gatgcgcagc aacgtctgga
     6841 tatggtgaaa gagttcaacg aactgtgcca ctcacacggt ctggtaagca tcattgagcc
     6901 agtcgtccgc ccaccgcgtc gtggcgataa attcgatcgc gaacaagcga tcatcgatgc
     6961 cgccaaagag ctgggcgaca gtggcgctga cctctacaaa gttgaaatgc cgctttatgg
     7021 caaaggtccg caacaagagc ttctctgtgc ttcacaacgt ctgaatgacc atatcaatat
     7081 gccatgggtg atcctctctt ccggtgtcga cgaaaaactg ttcccgcgtg ccgtacgcgt
     7141 ggcaatgacg gcgggcgcat cgggattcct ggcaggccgt gcagtctggg catcggtcgt
     7201 cggtttacca gacaacgagc tgatgctgcg tgacgtttgc gcaccgaaat tacaacaact
     7261 tggcgatatc gtcgacgaaa tgatggctaa acgccgctaa gatgaggata aaaggaatga
     7321 aatggtttaa caccctaagc cacaaccgtt ggctggaaca ggaaaccgac tgcatctttg
     7381 attttggtaa aaattccgta gtgccgactg gttttggctg gttaggcaat aaagggcaaa
     7441 tcaaagaaga gatgggcacc catctgtgga tcaccgctcg tatgttgcac gtttattccg
     7501 ttgctgcggc gatgggtcga cctggcgctt actcgttggt tgatcacggt atcaaagcca
     7561 tgaacggcgc actgcgtgat aaaaaatatg gcggctggta tgcctgcgtg aatgacgagg
     7621 gcgtggtgga tgcctccaaa cagggctatc aacacttctt tgctctgctg ggtgccgcca
     7681 gcgccgtcac aacgggtcac ccggaagcgc gcaagctgct cgattacacc attgaaatta
     7741 tcgagaaata tttctggagc gaagaagagc agatgtgcct ggaatcctgg gacgaagcct
     7801 tcagcaaaac cgaagagtac cgcggcggca atgccaatat gcacgcggtg gaagctttct
     7861 tgattgttta tgacgtcact cacgacaaaa aatggctgga tcgcgcgatt cgcgtggctt
     7921 ccgtgattat ccacgacgtc gccagaaata atcattatcg cgttaacgaa cacttcgata
     7981 cccagtggaa tccgctgccg gattacaaca aagataaccc ggcgcatcgc ttccgcgcgt
     8041 tcggaggtac accaggccac tggatcgaat ggggccgttt aatgctgcac atccatgctg
     8101 ccctggaagc ccgttgcgaa caaccaccag catggctgct agaagatgcc aaaggtctgt
     8161 ttaacgccac cgtgcgcgat gcctgggcac ccgatggcgc ggacgggatt gtttataccg
     8221 ttgactggga aggaaaaccg gtggtccgcg aacgtgtacg ttggcctatc gtcgaagcaa
     8281 tgggtacggc ctacgcgctc tacaccgtca ctggcgatcg ccagtatgaa acctggtatc
     8341 aaacatggtg ggagtactgc attaagtacc tgatggacta tgaaaatggt tcctggtggc
     8401 aggagctgga tgcggacaat aaggtcacca ccaaagtctg ggacggcaaa caggatattt
     8461 atcacctgct gcattgcctg gtgatcccgc gtatcccgtt agcgcccggc atggctccag
     8521 cggttgcagc gggtctgctg gatattaatg cgaaataact gatctgaaaa tagggtgcgg
     8581 gttttcccct caccctaacc ctctccccaa aggggcgagg ggaccgtccg gtgcagtttt
     8641 tgggtttgac atcagcctca cgggaggctg tatgtcgtta attaaggtgc cttgtatgca
     8701 aatcaccaat atgcattgca gtggacagac cgtttcactt gccgcggggg attatcacgc
     8761 gaccattgtg accgtcggtg ctggtctggc tgaattaacc tttcagggtt gtcatttagt
     8821 catcccgcac aaacccgaag agatgccgct ggcgcatctg ggtaaagtgc ttattccgtg
     8881 gccgaaccgg atcgccaacg gctgttaccg ctatcagggg caggaatatc agttacccat
     8941 taatgaacat agttcaaaag ccgctattca cgggctgctt gcctggcgag actggcaaat
     9001 tagtgaatta accgccacca gcgttaccct gacagctttt ctgccgccca gttacggcta
     9061 tccgtttatg ctggcttcac aagttgttta ttcgctcaac gcacacacgg gtctttccgt
     9121 tgagatcgca agccagaata tcggcactgt cgcagcgcct tatggcgtgg gtattcatcc
     9181 ttatttaacc tgcaacttaa catctgttga tgaatatctg ttccagcttc cggcaaacca
     9241 ggtttatgcc gtcgatgagc acgcgaatcc gacaacgctt caccacgttg atgagctgga
     9301 tttaaatttc acgcaggcga aaaaaatagc cgcgacaaaa atcgaccaca cctttaaaac
     9361 cgctaatgat ttgtgggaaa tgaccattac ccatccacag caggcgcttt cggtaagcct
     9421 ttgttccgat caactctggg tgcaggttta tagcggtgaa aaattacagc gtcagggact
     9481 ggcagttgaa cctatgagtt gcccgcctaa cgcctttaat tcaggtatcg acttattatt
     9541 actggaatca ggaaaaccgc accgcctgtt ttttaatatt tacggacaga gaaaataact
     9601 tgtattgatt agcagtcaaa atcattgaag aacatttcac tcgctaaagg caacattatg
     9661 tatcttcaat ttattaagtt aaaaacagct cagcttctgt aaagttttta tatatcagtt
     9721 ttatgagaat cattttacat aagcctgtaa taacaggaag gcaacaatct cgttctcaac
     9781 tctatggaga ttagttatgg atacgccacg tccacagtta ttagattttc aatttcatca
     9841 gaataacgac agttttaccc tacattttca acaacgtctt attttaaccc atagcaaaga
     9901 taatccttgt ttatggattg gctcaggtat agcggatatc gatatgttcc gcggtaattt
     9961 cagcattaaa gataaactac aggagaaaat tgcgcttacc gacgccatcg tcagccagtc
    10021 accggatggt tggttaattc atttcagccg tggttctgac attagcgcca cgctgaatat
    10081 ctctgccgac gatcaggggc gtttattgct ggaactacaa aacgacaacc ttaaccacaa
    10141 ccgtatctgg ctgcgccttg ccgctcaacc agaggaccat atctacggct gcggcgaaca
    10201 gttttcctac ttcgatctgc gtggcaaacc gttcccgcta tggaccagtg aacaaggcgt
    10261 tggtcgcaac aaacaaacct atgtcacctg gcaggccgac tgcaaagaaa atgcgggcgg
    10321 cgactattac tggactttct tcccacagcc tacgtttgtc agcacgcaga agtattactg
    10381 ccatgttgat aacagttgct atatgaactt cgactttagt gccccggaat accatgaact
    10441 ggcgctgtgg gaagacaaag caacgctgcg ttttgaatgt gctgacacat acatttccct
    10501 gctggaaaaa ttaaccgccc tgctgggacg ccagccagaa ctgcccgact ggatttatga
    10561 cggagtaacg ctcggcattc agggcgggac ggaagtgtgc cagaagaaac tggacaccat
    10621 gcgtaacgcg ggcgtgaagg tcaacggcat ctgggcgcag gactggtccg gtattcgtat
    10681 gacctctttt ggcaaacgcg tgatgtggaa ctggaagtgg aacagcgaaa actacccgca
    10741 actggattca cgcattaagc agtggaatca ggagggcgtg cagttcctgg cctatatcaa
    10801 cccgtatgtt gccagcgata aagatctctg cgaagaagcg gcacaacacg gctatctggc
    10861 aaaagatgcc tctggcggtg actatctggt ggagtttggc gagttttacg gcggcgttgt
    10921 cgatctcact aatccagaag cctacgcctg gttcaaggaa gtgatcaaaa agaacatgat
    10981 tgaactcggc tgcggcggct ggatggctga cttcggcgag tatctgccca ccgacacgta
    11041 cctgcataac ggcgtcagtg ccgaaattat gcataacgcc tggcctgcgc tgtgggcgaa
    11101 gtgtaactac gaagcccttg aagaaacggg caagctcggc gagatccttt tctttatgcg
    11161 cgccggttct accggtagcc agaaatactc caccatgatg tgggcgggcg accagaacgt
    11221 cgactggagt ctcgacgatg gcctggcgtc ggttgtcccg gcggcgctgt cgctggcaat
    11281 gaccggacat ggcctgcacc acagcgacat tggcggttac accaccctgt ttgagatgaa
    11341 gcgcagcaaa gagctgctgc tgcgctggtg cgatttcagc gccttcacgc cgatgatgcg
    11401 cacccacgaa ggtaaccgtc ctggcgacaa ctggcagttt gacggcgacg cagaaaccat
    11461 cgcccatttc gcccgtatga ccaccgtctt caccaccctg aaaccttacc tgaaagaggc
    11521 cgtcgcgctg aatgcgaagt ccggcctgcc ggttatgcgc ccgctgttcc tgcattacga
    11581 agacgatgcg cacacttaca ccctgaaata tcagtacctg ttaggtcgcg acattctggt
    11641 cgctccggtg catgaagaag gccgtagcga ctggacgctc tatctgccgg aggataactg
    11701 ggtccacgcc tggacgggtg aagcgttccg gggcggggaa gttaccgtta atgcgcccat
    11761 cggcaagccg ccggtctttt atcgcgccga tagcgaatgg gcggcactgt tcgcgtcgtt
    11821 aaaaagcatc taatcacatc cgcccgtggg gaaactcatg ggcaaaggga gaacatcgat
    11881 gagtcacatc acaacggaag atccagcaac tttacgcctg ccctttaaag agaaactctc
    11941 ttacggtatt ggcgacctgg cctctaacat cctgctggat atcggtacgc tttatctttt
    12001 gaagttttat accgacgttc tggggctgcc aggcacctat ggcggcatta tctttttgat
    12061 ttcaaaattc tttactgcgt ttaccgatat gggtaccggc attatgttgg attcccgacg
    12121 caagatcggt ccaaaaggta agttccgtcc ttttattctg tatgcgtcat tcccggtcac
    12181 cttactggcg atcgccaact ttgtcggcac accgtttgat gtcaccggta aaacggtgat
    12241 ggccactatt ctgtttatgc tttacggact gtttttcagc atgatgaact gctcctacgg
    12301 cgcaatggtt cccgctatca ccaaaaaccc caacgaacgc gcctcactgg cggcatggcg
    12361 tcagggcggc gctacgctgg gcctgctgct gtgcacggtg ggattcgtgc cagttatgaa
    12421 tcttatcgaa ggtaatcagc aacttggcta tatcttcgcc gccacgctgt tttcactgtt
    12481 tggcctgctg tttatgtgga tctgctactc gggcgtgaaa gagcgttatg tcgaaaccca
    12541 gcctgctaat ccggcgcaaa agccgggcct gctgcaatct ttccgcgcaa ttgcgggtaa
    12601 ccgcccgctg ttcattctgt gcattgccaa cctctgcact ttaggggcgt ttaacgtcaa
    12661 gctcgccatc caggtctatt acacccagta cgtgcttaac gatcccatcc tgttgtcgta
    12721 tatgggattt ttcagcatgg gctgtatttt catcggcgta ttcctgatgc ctgcctcagt
    12781 cagacgtttt ggcaagaaga aagtttatat cggcggcctg ctgatttggg tgctgggcga
    12841 tctgctcaac tatttcttcg gcggcggttc ggtcagcttc gtggcgttct cctgcctggc
    12901 gttctttggc tcagcgtttg ttaacagcct gaactgggcg ctggtttccg acaccgtcga
    12961 gtacggcgag tggcgcaccg gcgtgcgttc ggaaggaacg gtctacaccg gttttacctt
    13021 ctttcgcaaa gtgtctcagg cgctggctgg tttcttcccc ggctggatgc tgacgcaaat
    13081 tggctatgtg ccgaacgtcg cacaggctga ccacaccatt gaagggttac gccagttgat
    13141 cttcatctac ccaagcgcac tggcggtagt caccattgtg gcgatgggtt gcttctacag
    13201 cctgaacgag aagatgtatg tccgcattgt ggaagagata gaagcccgta aacgcacggc
    13261 gtaataataa taaacgacgc cctgcggggc gttataagga gtgattatat ctgaccataa
    13321 tccactgaca ttaaaactga atctgcggga aaaaatcgcc tatggtatgg gcgacgtcgg
    13381 ttcgaattta atgctctgca tcggtactct gtatctcctc aaattttata ccgatgaatt
    13441 agggatgcct gcttactatg gcgggattat ctttctggtg gcgaagtttt ttaccgcgtt
    13501 taccgatatg ctcaccggat ttttactcga ctcgcggaaa aatattgggc ctaaaggcaa
    13561 atttcgtccc tttattttat atgcagcggt tccggcggcg ttaattgcaa cgctccagtt
    13621 tatcgccacc accttttgtt tgccggttaa aacgacgatt gccaccgcgc tgtttatgat
    13681 gtttggactt tcatacagtc tgatgaactg ctcgtatggt gcgatgatcc cggcaattac
    13741 caaaaacccg aatgagcgcg cgcagctcgc ggcttaccgc cagggtggtg caaccatagg
    13801 gctattgatt tgtaccgtgg cgtttattcc gttgcagtcg cttttttctg actcaaccgt
    13861 cggttatgcc tgtgcggcac ttatgttctc cattggcggc tttattttta tgatgctgtg
    13921 ctacagaggc gtcaaagagc attatgtgga cacaacgcca accggacata aagccagtat
    13981 tctcaaatca ttttgcgcga tatttcggaa tccgccattg ctggttttat gcattgctaa
    14041 cttgtgtacc ctggcggcat ttaatatcaa actggcgatt caggtctatt acacccagta
    14101 tgtgctgaat gatattaatt tattgtcgtg gatgggattc ttcagtatgg gatgcatcct
    14161 catcggcgta ttactggtgc cattaactgt aaaatgtttt ggtaaaaaac aggtttatct
    14221 ggctggcatg gtgctgtggg cggtgggtga tatactgaat tatttctggg gaagtaactc
    14281 tttcactttc gtcatgttct cttgtgtcgc cttttttggc acggcctttg taaacagcct
    14341 gaactgggca ctggtaccag ataccgtaga ttacggtgaa tggaaaaccg gtattcgtgc
    14401 cgaaggttct gtttataccg gttatacctt ctttcgtaaa atttctgccg cacttgctgg
    14461 cttcttgcca ggcattatgc tgacgcaaat tggttatgtt cccaacatcg cgcaaagcga
    14521 tgcgacatta cagggtttgc gtcagctcat ttttatctgg ccttgtgcac tggcaattat
    14581 tgctgcattg acgatgggat tcttttacac actcaatgaa aaacggtttg cattgattat
    14641 tgaggaaatc aaccaacgca aaaataaaga gatggcgaca gaagaaaaaa cggcttccgt
    14701 aacgttataa atgtcaatag ccgctatttc catcctggtg ggtggcggcc tccctacgtt
    14761 taaaaaatgg acttattatg aaaaatatta atgcaataat tttattatct tctctgacct
    14821 ctgcctcggt atttgctggc gcatacgtcg aaaacaggga ggcatataat cttgcctccg
    14881 atcagggcga ggttatgcta cgtgtgggtt ataacttcga tatgggcgcg ggtattatgt
    14941 taaccaatac ctacaacttt cagcgagaag atgaactaaa acatggatat aacgaaattg
    15001 aaggctggta tccgttattt aaaccaaccg ataaattaac catccagccc ggtggcttaa
    15061 ttaatgataa gagtatcggt tcgggtggtg cagtgtatct ggacgtcaac tataaatttg
    15121 taccatggtt taatctgaca gtacgaaatc gctataacca taataactat agttcaacag
    15181 atttgagcgg ggaactggat aataatgaca cctatgaaat tggcacctac tggaatttta
    15241 aaatcaccga taaattttcc tatacatttg agccacatta cttcatgcga gtgaatgact
    15301 ttaatagtag caacgggaaa gatcatcatt gggaaatcac taacaccttc cgttaccgta
    15361 ttaatgaaca ctggctccct tatttcgaat tgcgctggtt agaccgcaac gtcgaaccgt
    15421 accaccgcga gcaaaaccag atccgtatcg ggacgaagta tttcttctga tgtgaaactg
    15481 ccggatgcgg cgtaaacgcc ttatccggcc tacggttgat acggttttca ttggccggat
    15541 taagcacatc cggccattaa ttaagcttca gccgccagca tctctttctt acgttggcta
    15601 atacgcttaa ccagcattac ggaaaccacc gcaccactga atgcgaagca ggccataatg
    15661 ccaaacacga ttttgtagcc aataatcccc ggatttaaat ccagaatgta gccatacaga
    15721 ctgaagcaga acatcgccgg agcgtaacca ataaagctac ccaacgccat cgccgcacct
    15781 gttttatttt cagcaatttt tgcttcgccg ataggtgcaa aaaatacggc tcgctgtgta
    15841 aagactatcg cgccaaagcc cagcgtacat gccatcccta aatagaccgg catactttcg
    15901 tgcggcagca taatcaacag tacgagcgca gcggtactaa tgataaaggt gtagcataga
    15961 tatttacttg gcgatttcag gatcttatct gaaatcatgc caccaatcgg tccaccaatc
    16021 attttcagac agtattggtt aatgatgccg taagccccca ccagcgcaac gggcaataga
    16081 tagatgtttt tcaggaatgg aatgaagaat gtcaggccac agtaaaccgc gtaaacgaag
    16141 aagacgttaa aagcgataag ccagatagtt ttgtctttca gcaccgaggt catactggtg
    16201 tttttcgatg ctccgtcttc ttttttcacc tcaacggacg gtgcctcttc tttgtcattc
    16261 agaacaaaga aaatgataat gcctacggca atcacaataa gggaatagaa ccagatgcct
    16321 gctttaaaac ccaataagcc actgccaaac caggtaaata ctgccaacgc agaaaatgcc
    16381 accacggtat cgacaatgcc acgccctgtt tcgaagaagc caaacaaccg accttgttgt
    16441 tcgctattgc ccaatcgact taccgacttg agcaagaccg gccagttcat catgtcgcaa
    16501 gtaacgccaa atagcgccca gacaaagagg atgccccaat accccggcat tgtcgtcaaa
    16561 taaacaccca gtaatcctgt cgcaatgagt gagaatgaca tggtgtatct gcgcggtagt
    16621 ttatcggcaa aataaataga cagaaaaaaa cccactgtcg tgacaaatga gtttaccgac
    16681 atagcattac caatttgacc attggtcaaa tggaaatatt cctgcatcgg gatataaaac
    16741 gcatctttca gcgacggtaa tttataaatt gtaccgccac acaatcttaa tagactaaat
    16801 aacgcccatt tctttttcgt gagcataaca tcccctaagc gatttttaaa atcactgttt
    16861 tataggcaat aagataccga ccattcatat ggaatggttt tattaactgc gtatgagtta
    16921 aaacttatta gtggttcaga agatggtttg cctcttgttt gatttgttgt agtacgttac
    16981 gcacatgatt tatctgatta attgcatctt ctttttcttt acgtaaccgc gcatccagta
    17041 aatgattttc tggcaatggt gtttcactca tctggcgata agggatccac ggattatcat
    17101 cttcgtcttc aaagcggaac gccggcgcat agtaatcaac ctcttcttcc aggccatatg
    17161 ccgtcacctg cggctcatca tcacccaggc agataaggtg cgttaataac gctttgaacg
    17221 gcatatcacc ctgacctgaa atacaggctt tatgacccag gccaccctgt tctttaacga
    17281 tcaaggcatc tttgatatgg acctgggtaa tatgcggtgc catcgttttt aaggcgtcga
    17341 tgggatgctc atttgcattg atcatgttcg caaaatcaaa cagtaaggaa agagactcca
    17401 tctcactttc tttgaccagc gacaccagct catgactttt taaatcttca tgctgctcga
    17461 tcgtaaaagt caggccgctg tcctgatacg tttcccgtac ataggcaatg tcgttagcga
    17521 taatcgataa tacgtcgcgc aaattacctt cataacgtgg ataaaaacgt acggacgatg
    17581 ccccagtttt caacgcgatg gcgacggctt cgtcaataga tgccttatct gaggcgctgg
    17641 tttcaatatg aatatcaagg ttcagacggc gggctttatc tccaaaggca gagagttctt
    17701 tatcgtccat attaccaaga gaaaaacgct cgccatccag aacatggatt ttgacgccac
    17761 gtagattatt ttcacttgcg atatcaagta aatcagcagg caacactctt tccagccgca
    17821 tatttaaatg aaaggcataa gcatgtaaat aaagaggaag agtgtcgaca cgttgtagaa
    17881 tctttcttgc gttatttatt gtcaccataa aacacctaca gattaatgat gaggtctacg
    17941 gcctcaaatc gccaatattc aatatctatc tggacaatat tgccttgctg gtcggcgcga
    18001 tgcttttcaa ttaagatcgc tggcataccc gcagaaccgc caagatactt gctgatatcg
    18061 cccggcattc tgacgggttt gaaaatcaat ttttttacta ccgtctcttt ttggtagcac
    18121 ttttcccaga ctgcagaaaa tgagtggttt tcaagttgtt caataaaacc cggagcaact
    18181 tcaggattaa tatatgtttc gtgatagaaa actttatgtc cttccagcgc cccccagccg
    18241 gtgatgcgat agagttcagt tgatggtgtc actgcaatca gtggcgcgag cgtctcggga
    18301 atatcgctgg tacggttttt ctcggtaaac ccccaggaag gctctcgtcc ttgctcaatt
    18361 gcggcccgct gaaagctcgc tgacagctcc ggactgtaat taaatcgcgg ctgggtcacg
    18421 aaccacccct tacgatcctt acggaagatt ttggattctg cttcgaggtt caacaacgcc
    18481 tggcgcagcg tcatacgttt aatgcccagc agttctccca gctctctttc tgaagggagt
    18541 tttccacccg gcgtggtgat gccatctttc aaccaccgat ccagtttctc ttttgcattt
    18601 tctacggtgg attgattctc cgccattgtt taatcctttt ggtttaaacc agatgaagca
    18661 tcttacgctt acgccaaaaa agaaagcaat taacggcgta tgactttgca gataattgtg
    18721 acaaagctct ctattgacgt aatcccagac ttataaggga gatactcgca ttggtgtgcg
    18781 ctcacgacag gtaaaaaaaa acctgccagc gatggcaggt tttttataac taaaaaacga
    18841 aattaatcgt ctttcggtgc gcggttggcg cgacgacgat cgttttcagt caggtgacgt
    18901 ttacgaatac ggatagaggt cggagtcact tctaccagtt cgtcatcatc gatgaactcc
    18961 agagcttgtt ccagagtcat gcggataggc ggaaccagaa caacggcttc gtcagtaccg
    19021 gaagcacgca tgttggtcag tttcttacca gtcaggcagt ttaccgtcag gtcgttagag
    19081 cggctgtgaa taccgataat ctgaccttcg taaacttctg caccgtgacc gaggaacagc
    19141 ttaccgcgat cctgcagacc gaacagcgcg aacgcgaccg ctttaccctg accgttagag
    19201 atcagtacgc cgttctgacg ctgacccact tcacccggac gtacgtcgtc gtagtggctg
    19261 aaggtggagt acagcagacc agtaccggaa gtcatggtca tgaactcaga acggaagcca
    19321 atcagaccac ggcttgggat cacgtagtcg agacgtacgc ggcctttacc gtctggattc
    19381 atgtttttca ggtcgccttt acgttcgccc agcgcctgca ttacagaacc ctgatgctgt
    19441 tcttcaacgt ccagagtcac gttttcatac ggctcttgtt tacgaccgtc gatttcacgg
    19501 aagataactt tcggacggga taccgccagt tcgaaacctt cacgacgcat gttttcgatc
    19561 agaacagaca ggtgcagttc gccacgacca gaaacgcgga acgcatcggc gtcttcggtt
    19621 tcttctacgc gcagcgcaac gttgtgtacc agttctttgt tcagacgatc caggatctga
    19681 cgagacgtta cgaacttacc ttctttaccg cagaacggcg aggtgttaac gcagaagaac
    19741 atagaaacgg tcggctcatc aacggagagt gccggcagcg cttcaacgtt ttgcgtgtcg
    19801 caaacggtgt cagaaatgtt cagttcgcca aggcccgtga tcgcaacgat atcgccagct
    19861 tccgccagat cggtttcgat acgttccaga ccgaggtggc ccagcacttt accgactttc
    19921 gcgttgcggg ttttgccttc gctatcgatg atagtgacct gctggttcgg cttcacttta
    19981 ccgcgcttga tgcggccaat gccgataacg ccaacatagc tgttgtaatc gagctgagaa
    20041 atctgcatct ggaacggacc gtcaaggtca acgtccggcg caggaacgtg atcaacaatc
    20101 gcctgataca gcggggtcat gtcttccgcc atatcttcgt ggtccagacc cgcgataccg
    20161 ttcagcgcag aagcgtaaac gatcgggaag tccagctgct cgtcggtcgc gtcgaggtta
    20221 acgaacagat cgaatacctg atccacaacc caatcaggac gcgcgccagg gcggtcaact
    20281 ttgttgataa caacaatcgg cttcaggccg taagcaaacg cttttttggt tacgaagcgc
    20341 gtttgcggca tcgggccgtc aaatgcgtca accaccagca gcactgagtc taccatggac
    20401 attacacgtt caacttcacc accgaagtcg gcgtgccccg gggtatcaac gatgttgata
    20461 cggtaatcat tccatttgat agcggtgttt ttcgcgagga tggtaatccc acgctctttc
    20521 tccaaatcgt tggagtccat cacgcgctct tgggtttcgg cacgagagtc gaacgtaccg
    20581 gattgttgga gcagcttgtc taccagggtg gttttaccat ggtctacgtg cgcgatgatg
    20641 gcgatattac gcaatttttc gatcacaact ttgcctcagg cattagaaat agcgcgttat
    20701 tgtacacgga ttaatcgcac tacaaaacag gatcacaaac atcctccgca aacaagtatt
    20761 gcagagtccc tttgtgatcg ctttcacgga gcataaaaag ggttatccaa aggtcattgc
    20821 accaacatgg tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tcacatcgtg
    20881 gtgcagccct tttgcacgat ggtgcgcatg ataacgcctt ttaggggcaa tttaaaagtt
    20941 ggcacagatt tcgctttatc ttttttacgg cgacacggcc aaaataattg cagatttcgt
    21001 taccacgacg accatgacca atccaggaga gttaaagtat gtccgctgaa cacgtactga
    21061 cgatgctgaa cgagcacgaa gtgaagtttg ttgatttgcg cttcaccgat actaaaggta
    21121 aagaacagca cgtcactatc cctgctcatc aggtgaatgc tgaattcttc gaagaaggca
    21181 aaatgtttga cggctcctcg attggcggct ggaaaggcat taacgagtcc gacatggtgc
    21241 tgatgccaga cgcatccacc gcagtgattg acccgttctt cgccgactcc accctgatta
    21301 tccgttgcga catccttgaa cctggcaccc tgcaaggcta tgaccgtgac ccgcgctcca
    21361 ttgcgaagcg cgccgaagat tacctgcgtt ccactggcat tgccgacacc gtactgttcg
    21421 ggccagaacc tgaattcttc ctgttcgatg acatccgttt cggatcatct atctccggtt
    21481 ctcacgttgc tatcgacgat atcgaaggcg catggaactc ctccacccaa tatgaaggtg
    21541 gtaacaaagg tcaccgtccg gcagtgaaag gcggttactt cccggttccg ccggtagact
    21601 ctgctcagga tattcgttct gaaatgtgtc tggtgatgga acagatgggc ctggtggttg
    21661 aagcccatca ccacgaagta gcgactgctg gtcagaacga agtggctacc cgcttcaata
    21721 ccatgaccaa aaaagctgac gaaattcaga tctacaaata tgttgtgcac aacgtagcgc
    21781 accgcttcgg taaaaccgcg acctttatgc caaaaccgat gttcggtgat aacggctccg
    21841 gtatgcactg ccacatgtct ctgtctaaaa acggcgttaa cctgttcgca ggcgacaaat
    21901 acgcaggtct gtctgagcag gcgctgtact acattggcgg cgtaatcaaa cacgctaaag
    21961 cgattaacgc cctggcaaac ccgaccacca actcttataa gcgtctggtc ccgggctatg
    22021 aagcaccggt aatgctggct tactctgcgc gtaaccgttc tgcgtctatc cgtattccgg
    22081 tggtttcttc tccgaaagca cgtcgtatcg aagtacgttt cccggacccg gcggctaacc
    22141 cgtacctgtg ctttgctgcc ctgctgatgg ccggtcttga tggtatcaag aacaagatcc
    22201 atccgggcga agcaatggac aaaaacctgt atgacctgcc gccagaagaa gcgaaagaga
    22261 tcccacaggt tgcaggctct ctggaagaag cactgaacga actggatctg gaccgcgagt
    22321 tcctgaaagc cggtggcgtg ttcacggatg aagcgatcga tgcgtacatc gctctgcgtc
    22381 gcgaagaaga tgaccgcgtg cgtatgactc cgcatccggt agagtttgag ctgtactaca
    22441 gcgtctaata gttgaagttg tactacccgg cgcgaacaac gccgggattt agttgccgtg
    22501 gaaactttca gcccatctct gcatgggctt ttttctccgt caattctctg atgcttcgcg
    22561 ctttttatcc gtaaaaagct ataatgcact aaaatggtgc aacctgttca ggagactgct
    22621 ttatggcaac aggcacgcag cccgatgctg ggcagatcct caactcgctg attaacagta
    22681 ttttgttaat cgatgacaac ctggcgatcc attacgccaa ccccgccgcg caacaactgc
    22741 tcgcccaaag ctcccgcaaa ttgtttggta caccgttacc ggaactgttg agctacttct
    22801 cattaaatat cgagctgatg caagaaagtc tggaggcggg gcaaggtttt accgataacg
    22861 aagtgacgct ggtcatcgac gggcgctcgc atatcctttc tgtgacggcc cagcgtatgc
    22921 cggacggcat gatcctgctg gagatggctc cgatggataa ccagcgccgc ttaagtcagg
    22981 aacagctaca gcacgcccag caggttgctg cccgtgattt agtgcgtggc ctggcacatg
    23041 agattaaaaa tccgcttggc ggtttacgtg gcgcggcgca gctgctcagc aaagcgttac
    23101 ctgacccatc actactcgaa tataccaaag tgattatcga acaggcggac cggctgcgaa
    23161 atctggtcga ccgtctgttg gggccgcagc tgcccggtac gcgcgttacc gaaagtattc
    23221 acaaagtggc tgaacgcgtg gtaacgctgg tgtcgatgga actgccggac aacgtgcggt
    23281 tgattcgtga ttacgatccc agcctgccgg aactggcgca cgacccggat caaattgaac
    23341 aggtcttgct gaatattgtg cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa
    23401 tcattctgcg tacccgcacc gcgtttcaac tgaccttaca cggcgagcgc taccggctgg
    23461 cggcgcggat tgatgtggaa gataacgggc cgggcattcc gcctcatttg caggatacgc
    23521 tgttttaccc gatggtcagc ggccgcgaag gtggcaccgg gcttggctta tccatcgctc
    23581 gtaatttgat tgatcagcat tcaggcaaaa ttgaatttac cagttggcca gggcataccg
    23641 agttctcggt ttacctgcct atcaggaaat aaaggtgacg tttatgcaac gagggatagt
    23701 ctgggtagtc gatgacgata gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc
    23761 gggtttaacc tgtacgacat ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa
    23821 aacgccggat gtgctgcttt cagatatccg tatgccggga atggacgggc tggcgctgct
    23881 caagcagatt aaacagcgcc atccaatgct tccggtcatc attatgaccg cacattccga
    23941 tctggatgct gccgtcagcg cctatcaaca aggggcgttt gattatctgc ccaaaccgtt
    24001 tgatatcgac gaagcagtgg cgctggttga gcgcgctatc agtcattacc aggaacagca
    24061 gcagccgcgt aatattcagc ttaacggccc aacgaccgat atcatcggcg aagcgccagc
    24121 catgcaggac gtgttccgta ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat
    24181 taacggcgaa tccggcaccg gtaaagaact ggtcgctcat gccctgcatc gccacagtcc
    24241 gcgcgccaaa gcgccgttta tcgcgctgaa tatggcagct atcccaaaag atttgatcga
    24301 atcagaactg tttggccacg agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg
    24361 gcgttttgaa caggccgatg gcggtacatt attcctcgac gaaattggtg atatgccgct
    24421 ggatgtgcag acgcgtttgc tgcgcgtgct ggcagacggt cagttttacc gcgttggcgg
    24481 ctatgcgccg gtgaaagtgg atgtgcggat tatcgctgcc actcaccaga atctcgaaca
    24541 gcgagtgcag gaaggtaagt tccgtgagga tctgttccac cgcctgaacg ttatccgcgt
    24601 tcatctgccg ccgctgcgcg aacgtcggga agatattccc cgtctggcgc gccatttttt
    24661 acaggttgcc gcgcgcgaac tgggcgtaga agcgaagtta ctgcatccgg aaaccgaagc
    24721 tgctctgacg cgtctggcgt ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg
    24781 gctaacggtg atggccgccg ggcaggaagt gttgattcag gatttgcccg gcgaactgtt
    24841 tgaatcaacg gttgcggaga gtacttcgca aatgcaaccg gacagctggg cgacgcttct
    24901 tgcgcagtgg gcagacagag cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca
    24961 gccagagctg gagcggacgt tactgacgac cgcgttgcga catacgcagg ggcataaaca
    25021 ggaagcggcg cggctactcg gctggggccg caacaccctg acgcgtaagt taaaagagct
    25081 ggggatggag tgattcacag cttgtgtgta aagattgatt attgagcgca aattgctggt
    25141 attttacgct ttactgttcc gataagttca gtatgatctt gcccggaaaa cggggagagt
    25201 cattatgctg gaatcaataa ttaatctggt atcgagtggc gcagttgaca gccacacacc
    25261 gcaaactgcc gttgctgccg tgctgtgtgc cgcaatgatt gggctgttta gttgagtttc
    25321 agattgctga tgaagtgctc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc
    25381 tacaaaagca atattttcaa caggttttgt aggcctgata agcgtagcgc atcaggcaat
    25441 cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa gcgccattca
    25501 ctatttaaat cacccgtgag aactgctgca tccgcgcttt ctggcgcaga taggtatcaa
    25561 agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt attcccttct
    25621 catccacatc caccagccca tcttttgcta acggggcgag cagcttgaga tcttccgcaa
    25681 agtaatcagc gaagtgcaaa tcccactgtt tctcaatagg ggcgtaatcc agacggaagt
    25741 tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt agcgcaatac
    25801 cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc aactctttct
    25861 ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc cccagcagat
    25921 cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct tcacgctggg
    25981 ccaccgccag ctcgtcatcc ggacgggcaa agtgatccat accgataaac tgatagcccg
    26041 attgcgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc ggactcggca
    26101 ggtcagcatc tttgattttg cgctgagcag caaaaatggt cggcagatgc gcgtagttaa
    26161 agacactcag acgatcgggg ttcagctccg ccacacgttt cagggtaaag gcgaaactct
    26221 ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct
    26281 cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg ttaaccagac
    26341 gttgcacttc tttgttgaag tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc
    26401 gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc gaaatctccg
    26461 catcggcatt gaactggaag ttttcgcgca gcagcttcat caggcggctg atttgcgctt
    26521 tattcagata cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga cgcccggcga
    26581 acagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga tcggccttgt
    26641 gctgctggcg agtaacaatc ttattgcaac cgcagaagta acaaagctta tggcagaacg
    26701 ggatatgtac gtagagagat aatggacgct caggatagcg cgccacggct tgtaaaaacg
    26761 cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc
    26821 cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt acagacatgc
    26881 tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc cgtttcgggt
    26941 catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt taacgaataa
    27001 ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc ccacggcggc
    27061 aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt cttcttcctc
    27121 ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa tacgatccag
    27181 tttagcatcc acccaggatt gctcttcggc actcagggtt tcgcctgctt ccagacgttc
    27241 cagcagcgca tccagacgct catccgtttc cagtaattcc aactccgcct gcggtgaaag
    27301 cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca cgcccaatgg
    27361 aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctggcctt tgctgcctga
    27421 cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg
    27481 cttgcgatca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg
    27541 acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt tagccgtttt
    27601 attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga
    27661 ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg
    27721 ccgccaacac accctgtttt tcctttagcc ggaacaatcc gtgttgattc catttccgtt
    27781 taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc
    27841 ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgctacttta
    27901 cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta
    27961 tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg
    28021 ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg
    28081 ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca aagctttgac
    28141 tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg
    28201 cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt
    28261 ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta
    28321 cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct gacggcaagc
    28381 gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat
    28441 ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc
    28501 taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag
    28561 acagcaatat cgccgttctg gtgctgctga ccaaagcgga caaactggca agcggcgcac
    28621 gtaaagcgca attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg
    28681 ttgaaacgtt ttcttcgttg aagaaacaag gcgtggacaa gctgcggcag aaactggata
    28741 cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc
    28801 ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac
    28861 cgttggcctg ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa
    28921 aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga
    28981 agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc
    29041 ttttttgttc agttcagaaa gcactttttg tgattttttt tcagttgttg cataggaatt
    29101 tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa aagggcatgt
    29161 tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc caccagcaac
    29221 ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc gacggcatca
    29281 acatcatctt tatgaacttc aaataccagt tcatcgtgta cctgcatgat catacgtaca
    29341 cgcggttgct cagcctgtaa ccacgcatca acggcaatca tcgcccgttt gataatgtcg
    29401 gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc acgacgagca
    29461 ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt ttcaacgtag
    29521 ccctgctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc agggtagcgt
    29581 tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg gaatgttcaa ttgccgcgcc
    29641 agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgctttcgc gctacggcgt
    29701 tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt tgcccggtgg
    29761 atatcttttc cttccgcgaa tgcggtcagc aagcctttgt cacgcgaaag atgcgccata
    29821 atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc tggcgcaata
    29881 aacgcctggc ggatacgacg accttcttcg ttacgcaccg gaatgttttg caggttagga
    29941 tcggttgacg ataaacgtcc cgttgcagtt actgcctggt gataagaggt atgcacacgc
    30001 ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt aggtcgattt cagcttcgcc
    30061 agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag ttcttccagt
    30121 acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt aatgccctgt
    30181 ttttcaaaga gaatggtttg taactgcttg gtggaagaaa ggttaaattc ctcacctgca
    30241 atttcatgcg ctttcttttc cagctcagcc agacgaaggg tgagctcttc agaatgattg
    30301 tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag caccggcacc
    30361 agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg cagatccggc
    30421 cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta acgtccggct
    30481 tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttaccagc aatctcttca
    30541 aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc gtgacgcccg
    30601 gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc acgcagttca
    30661 atgccgtagt tcgccagaat accgcgatcg tatttcaggt tttgcccgac cttcagcgcc
    30721 ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgcgaga gatttgatcg
    30781 ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg ctcgatagca
    30841 aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc ggtatcaaat
    30901 gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc ttcatcaagg
    30961 atggtgacgt agttgtcata agaaatcacc gttgccgtca cttctggtgc ttcgtctgca
    31021 acactggttt cctgtggctt cgcggctggt tttgcccctt tggcctgtaa ccatttgccc
    31081 gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc caacaactct
    31141 tccgctgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc aacgtcggtt
    31201 ttaatcgtcg ccagctggta tgagagataa gcaacttctt tgttttgctc gagcttcgct
    31261 gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc ggcatacagc
    31321 gtatccagtc cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc gacgcccggt
    31381 acgccaggaa tgttatcaga ggagtcaccc atcagcgcca ggaaatcgat gatcagttct
    31441 ggcggcacgc cgtacttatt caccacctct tccgggccga ggatggtatt cgtcatggta
    31501 ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc agtgctgatc
    31561 agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac gtcgtccgct
    31621 tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc gtgcaagggt
    31681 tcgatttgtg cacgcagatc gtccggcatt ggcgggcgat gtgatttgta atgttcaaac
    31741 agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg cgtcggttta
    31801 tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc ggtcggctcg
    31861 cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata agatgaacca
    31921 tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc tgtttatcag
    31981 attatgggta aaggatgcca cagacaggat gaaaacatga gtttttcacc gcttttcgtg
    32041 aaaatctttt gtagatcttc tggatcgctc gcaaatcgtc atgtggataa ctttgtgcat
    32101 aaattattta catcgctttt aatttattat catgcttttt acagcatgat gtttgtcttt
    32161 gtttttcatg cagttaaaaa taaatccatc cctaataatt gagataatgg gattttttct
    32221 aatgtg
//
LOCUS       DAOFQD010000052        31978 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.052, whole genome shotgun sequence.
ACCESSION   DAOFQD010000052 DAOFQD010000000
VERSION     DAOFQD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.052"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..245)
                     /locus_tag="QBF46_002363"
     CDS             complement(<1..245)
                     /locus_tag="QBF46_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674538.1"
                     /translation="MLTGDSYKDVKFMLRIFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIVLHYLVINMNCQRLRDSGF"
     gene            complement(304..1194)
                     /gene="rarD"
                     /locus_tag="QBF46_002364"
     CDS             complement(304..1194)
                     /gene="rarD"
                     /locus_tag="QBF46_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDQ6674539.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPISLNLLLIT
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1246..1713)
                     /locus_tag="QBF46_002365"
     CDS             complement(1246..1713)
                     /locus_tag="QBF46_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDQ6674540.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1878..2747
                     /gene="pldA"
                     /locus_tag="QBF46_002366"
     CDS             1878..2747
                     /gene="pldA"
                     /locus_tag="QBF46_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDQ6674541.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2880..4709
                     /gene="recQ"
                     /locus_tag="QBF46_002367"
     CDS             2880..4709
                     /gene="recQ"
                     /locus_tag="QBF46_002367"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDQ6674542.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRTKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4773..5393
                     /gene="rhtC"
                     /locus_tag="QBF46_002368"
     CDS             4773..5393
                     /gene="rhtC"
                     /locus_tag="QBF46_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDQ6674543.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5455..6075)
                     /gene="rhtB"
                     /locus_tag="QBF46_002369"
     CDS             complement(5455..6075)
                     /gene="rhtB"
                     /locus_tag="QBF46_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDQ6674544.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIGFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARNA"
     gene            6186..7208
                     /gene="pldB"
                     /locus_tag="QBF46_002370"
     CDS             6186..7208
                     /gene="pldB"
                     /locus_tag="QBF46_002370"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDQ6674545.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7216..8016
                     /gene="yigL"
                     /locus_tag="QBF46_002371"
     CDS             7216..8016
                     /gene="yigL"
                     /locus_tag="QBF46_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDQ6674546.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFQYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8092..8991
                     /gene="bioP"
                     /locus_tag="QBF46_002372"
     CDS             8092..8991
                     /gene="bioP"
                     /locus_tag="QBF46_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDQ6674547.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNTQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8879..9832)
                     /gene="metR"
                     /locus_tag="QBF46_002373"
     CDS             complement(8879..9832)
                     /gene="metR"
                     /locus_tag="QBF46_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDQ6674548.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9950..12211
                     /gene="metE"
                     /locus_tag="QBF46_002374"
     CDS             9950..12211
                     /gene="metE"
                     /locus_tag="QBF46_002374"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDQ6674549.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12251..13066)
                     /locus_tag="QBF46_002375"
     CDS             complement(12251..13066)
                     /locus_tag="QBF46_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDQ6674550.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13328..14089
                     /gene="udp"
                     /locus_tag="QBF46_002376"
     CDS             13328..14089
                     /gene="udp"
                     /locus_tag="QBF46_002376"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDQ6674551.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14230..15657
                     /gene="rmuC"
                     /locus_tag="QBF46_002377"
     CDS             14230..15657
                     /gene="rmuC"
                     /locus_tag="QBF46_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDQ6674552.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15752..16507
                     /gene="ubiE"
                     /locus_tag="QBF46_002378"
     CDS             15752..16507
                     /gene="ubiE"
                     /locus_tag="QBF46_002378"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDQ6674553.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16521..17126
                     /gene="ubiJ"
                     /locus_tag="QBF46_002379"
     CDS             16521..17126
                     /gene="ubiJ"
                     /locus_tag="QBF46_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDQ6674554.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLVPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17123..18763
                     /gene="ubiB"
                     /locus_tag="QBF46_002380"
     CDS             17123..18763
                     /gene="ubiB"
                     /locus_tag="QBF46_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDQ6674555.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18842..19111
                     /gene="tatA"
                     /locus_tag="QBF46_002381"
     CDS             18842..19111
                     /gene="tatA"
                     /locus_tag="QBF46_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDQ6674556.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19115..19630
                     /gene="tatB"
                     /locus_tag="QBF46_002382"
     CDS             19115..19630
                     /gene="tatB"
                     /locus_tag="QBF46_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDQ6674557.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19633..20409
                     /gene="tatC"
                     /locus_tag="QBF46_002383"
     CDS             19633..20409
                     /gene="tatC"
                     /locus_tag="QBF46_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDQ6674558.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFDVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20451..21233
                     /gene="tatD"
                     /locus_tag="QBF46_002384"
     CDS             20451..21233
                     /gene="tatD"
                     /locus_tag="QBF46_002384"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDQ6674559.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21230..21718)
                     /gene="rfaH"
                     /locus_tag="QBF46_002385"
     CDS             complement(21230..21718)
                     /gene="rfaH"
                     /locus_tag="QBF46_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDQ6674560.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21885..23378
                     /gene="ubiD"
                     /locus_tag="QBF46_002386"
     CDS             21885..23378
                     /gene="ubiD"
                     /locus_tag="QBF46_002386"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDQ6674561.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDNDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23424..24125
                     /gene="fre"
                     /locus_tag="QBF46_002387"
     CDS             23424..24125
                     /gene="fre"
                     /locus_tag="QBF46_002387"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDQ6674562.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24408..25571)
                     /gene="fadA"
                     /locus_tag="QBF46_002388"
     CDS             complement(24408..25571)
                     /gene="fadA"
                     /locus_tag="QBF46_002388"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDQ6674563.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25581..27770)
                     /gene="fadB"
                     /locus_tag="QBF46_002389"
     CDS             complement(25581..27770)
                     /gene="fadB"
                     /locus_tag="QBF46_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDQ6674564.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27960..29291
                     /gene="pepQ"
                     /locus_tag="QBF46_002390"
     CDS             27960..29291
                     /gene="pepQ"
                     /locus_tag="QBF46_002390"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDQ6674565.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPISFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29291..29905
                     /locus_tag="QBF46_002391"
     CDS             29291..29905
                     /locus_tag="QBF46_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDQ6674566.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29944..31395
                     /gene="trkH"
                     /locus_tag="QBF46_002392"
     CDS             29944..31395
                     /gene="trkH"
                     /locus_tag="QBF46_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDQ6674567.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31407..31952
                     /gene="hemG"
                     /locus_tag="QBF46_002393"
     CDS             31407..31952
                     /gene="hemG"
                     /locus_tag="QBF46_002393"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDQ6674568.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7426 a         8153 c         8752 g         7647 t
ORIGIN      
        1 atgaaaccac tatcacggag tcgctggcaa ttcatgttga tgacgagata atggagtacg
       61 atggtagaga ctataacaag aaagcctgct tctccatcgt taaaaaagat aataagaaag
      121 gcaaaaatga aatttattaa aataaatgaa aatatataac gacgtctgga aatcttaccg
      181 ttagatgttg ggataaatat acgtaacata aattttacat ccttgtatga gtctccggtc
      241 agcatggcaa tatgcccact ctcatgcaag agtcggcata ttttttcaga atatatttat
      301 tttttatttg gacgttctac gctgcgtata aatcgcatcc atcacaaaaa ttgccagcgc
      361 cacccaaata aaggcgaagg tcaccatctt atcggcaccc ggtttttcac cataaaacgt
      421 cacagccagc aggaacatca gcgtcgggcc aatgtactga aaaaagccta acgttgagag
      481 acgcaggcgt gtcgcagccg cagtaaaaca caacagcggt acggtagtga caataccggc
      541 ggtgatcagc agtaaattca gcgaaatcgg gttttgcccc atatggctgg ttgagctgtc
      601 ggcaatagca aacaggtaaa ttgccgccac gggcagcagc cacatggttt caattaacat
      661 gccggtttgc gcttcaacgg caatcttctt gcgtaccaga ccgtagaagg caaaactaaa
      721 tgccagtccc agcgcgataa taggtagcga accaaaagtc cacagctgga ctaacacgcc
      781 acatatcgcc agaatcaccg ccagccattg catccggcgg aatcgctcgc cgaggaaaat
      841 catccccagc acaatgttca ccagcgggtt aataaagtaa ccaaggctcg cttccagcat
      901 atggtgattg ttcaccgccc agataaacag tagccagttg ccaccaatca gcacggcaga
      961 gactgccagc ataaaaattt tctgtggcgt ctgaatcagc gtttttaaat aggaccactg
     1021 gcggcaaatg ctcatcagca ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag
     1081 gatttcatcg gcgggcacgt agtaaatcaa cttgaagtac gctggcgcta taccccaaat
     1141 aaaataagcg gcaagagcga gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa
     1201 tactcatttt taattggtaa cagcagttta cctgctttta tgtcttcaac ctaccatata
     1261 ggtggcggtg gcactggcaa tataaagctg ttcttcattg tgtaattcaa cgcgggcgac
     1321 ggcgacttta ttgcctgcac gcaacaggct actggtagca gtaaaacgct cgcccctgcc
     1381 tgggcgcaga taatcaacgc gaagatcaat ggtccccatc cgcgatagcc gctggcgtag
     1441 ttcatcttca ctgatggttt cgtggcgggt taaggtactt cccacgcaca ccagaccggc
     1501 ggcgacatcc agcgccgacg caatgactcc gccgtgcaaa atgctttgcg cccagttacc
     1561 caccatcatc ggctgatttt taaaggccag ctgtgcgaac tctttttcgt aacgctccag
     1621 ctccatccct aacgcgcggt taaatggcat gtgataaaca aacatctcac ccactaattt
     1681 cagggcttgt tcagcggtca gtacggcaga catatcatcc ttacacttca ttggttaatg
     1741 aaatgttgat tttatgcttc tttgttgttg atttctactt taggaaggga taactaacgg
     1801 ctatggagtt aagtatgtaa aatagcccgc agaaaaatat tcaccttatc aataattcgt
     1861 tacggagaac acgaccgatg cggactctgc agggctggtt gttgccggtg tttatgttgc
     1921 ctatggcagt atatgcacaa gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc
     1981 gtggcagtat tatcgccaat atgctgcagg agcatgacaa tccgttcacg ctctatcctt
     2041 atgacaccaa ctacctcatc tacacccaaa ccagcgatct gaacaaagaa gcgattgcca
     2101 gttacgactg ggcggaaaat gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat
     2161 ttccgctgtg gcgtgggatt ttaggcccga actcggtgtt gggtgcgtct tatacgcaaa
     2221 aatcctggtg gcaactgtcc aatagcgaag agtcttcacc gtttcgtgaa accaactacg
     2281 aaccgcaatt gttcctcggt tttgccaccg attaccgttt tgcaggttgg acgctgcgcg
     2341 atgtggagat ggggtataac cacgactcta acgggcgttc agacccgacc tcccgcagct
     2401 ggaaccgcct ttatactcgc ctgatggcag aaaacggtaa ctggctggta gaagtgaagc
     2461 cgtggtatgt ggtgggtaat actgacgata acccggatat caccaaatat atgggttact
     2521 accagcttaa aatcggctat cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca
     2581 actggaacac cggctacggc ggcgcggagt taggcttaag ttacccgatc accaaacatg
     2641 tgcgccttta tactcaggtt tacagcggct atggcgaatc gctcatcgac tataacttca
     2701 accagacccg tgtcggtgta ggagttatgc taaacgactt gttttgatga acggttgagt
     2761 ggttggcaaa tctggaaccc agcatccagg attaccctct cagagactaa aagcattgca
     2821 gtttctcgcg caggcgctga aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg
     2881 tggcgcaggc ggaagtgttg aatctggagt ccggagctaa acaggtttta caagaaacct
     2941 ttggctacca acagtttcgc cccggccagg aagaaattat cgacactgtg ctttccggtc
     3001 gcgattgcct ggtcgtcatg cccaccggtg gcggaaaatc cctttgctat caaattcctg
     3061 ccttattgct aaacggcctt accgtggttg tttcaccgct gatttcgttg atgaaagatc
     3121 aggtggatca actgcaagcc aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc
     3181 gcgaacagca acttgaagtg atgacaggct gccgcaccgg gcaaattcgt ctgctttata
     3241 tcgccccgga acgcctgatg ctggataact ttcttgagca tctggcgcac tggaatccgg
     3301 tgttattagc cgttgatgaa gcgcactgta tctcccaatg gggccacgat ttccgcccgg
     3361 aatatgccgc gctcggtcag ttgcgccagc ggttcccgac gctgccgttt atggcgctga
     3421 ccgccacagc cgacgacacc acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc
     3481 cgctgattca aatcagcagt tttgaccgtc cgaatattcg ctacatgctg atggagaaat
     3541 tcaaaccgct cgatcagttg atgcgctacg tgcaggaaca gcgcggtaag tccggcatta
     3601 tctactgcaa cagccggacg aaagtagaag acaccgctgc gcgcctgcaa agcaagggta
     3661 ttagcgcggc ggcctatcat gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga
     3721 aattccagcg cgatgacctg caaattgtgg tggcgacggt ggcgttcggc atgggcatca
     3781 ataaaccaaa cgttcgcttc gtggtccact ttgatattcc gcgcaatatc gaatcctatt
     3841 atcaggaaac cggtcgcgcc gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg
     3901 atccggctga tatggcgtgg ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc
     3961 aggatatcga gcgccacaaa ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc
     4021 gtcgtctggt attgctgaac tattttggcg aagggcgtca ggagccgtgc gggaactgcg
     4081 atatctgcct cgatccgccg aaacagtacg acggttcaac cgatgctcag attgcccttt
     4141 ccaccattgg tcgtgtgaat cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg
     4201 gtgctaataa ccagcgtatc cgcgactatg gtcatgacaa actgaaagtc tatggcatgg
     4261 gccgtgataa aagccatgaa cattgggtga gcgtgatccg ccagctgatt cacctcggcc
     4321 tggtgacgca aaatattgcc cagcattctg ccctacaact gacagaggcc gcgcgcccgg
     4381 tgctgcgcgg cgaatcctct ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga
     4441 aagcgatgca gaaatcgttc ggcggcaact atgatcgcaa actgttcgcc aaattacgca
     4501 aactgcgtaa atcgattgcc gatgaaagca atgtcccgcc gtacgtggtg tttaacgacg
     4561 caaccttgat tgagatggct gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta
     4621 acggcgttgg gatgcgcaag ctggaacgct ttggtaaacc gtttatggcg cttatccgcg
     4681 cgcatgttga cggcgacgac gaagagtagt cagcggcata aaaaagtgcc aggatgaaga
     4741 ctccgtaaac gtttcccccg cgagtcaaat gtatgttgat gttatttctc accgtcgcca
     4801 tggtgcatat tgtggcgctt atgagccccg gtcccgattt cttttttgtc tctcagaccg
     4861 ctgtcagtcg ttcccgtaaa gaagcgatga tgggcgtgct gggcattacc tgcggtgtaa
     4921 tggtttgggc tgggattgcg ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc
     4981 tgcatacgct gattatggtt ggcggtggcc tgtatctctg ctggatgggt taccagatgc
     5041 tacgcggtgc actgaaaaaa gaggctgtat ctgcgcctgc accacagatt gagctggcga
     5101 aaagcgggcg cagtttcctg aaaggtttac tgaccaatct cgctaatccg aaagcgatta
     5161 tctactttgg ctcggtgttc tcattgtttg tcggtgataa cgttggcact accgcgcgct
     5221 ggggcatttt tgcgctgatc attgtcgaaa cactggcgtg gtttaccgtc gttgccagcc
     5281 tgtttgccct gccgcaaatg cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt
     5341 ttgccggggc gttatttgcc ggatttggca ttcatttgat tatttcgcgg tgatgccaga
     5401 cgcgtcttca gagtaagtcg gataaggcgt ttacgccgca tccgacatta tttttcacgc
     5461 attcctcgcc gatgctaaca gcgctcccac cagcataaac aacgagccga aaatcttatt
     5521 cagcgccttc atctgctttg gtcctttaat ccatagagca atccgttgag caagggtggc
     5581 gtaaccgatc atcacaataa tatcgactac aatagtggtg acgccgagca cgatatactg
     5641 catcagttgc ggctgctgtg gcatgatgaa ttgcggaaat agcgccgcca gaaacccaat
     5701 acttttggga ttggtgagat tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg
     5761 agtagaggcc agcgatttaa ggtcaattgc accagcggcg cgccactgct ggattcccag
     5821 ccaaatcaag taagccgcgc ctgcccactt caacacttca aacgcaatca ctgagcggga
     5881 aaatagcgtc cccaacccca cgccaaccag cacaatatga atcgccagtc cggtctgaag
     5941 cccagcaata gacgccaccg cgccgcgata accgtggttg agcgaggtgg tcatagtgtt
     6001 gattgcacca gagcctggcg acaggcttaa aatgatcgat gtcagcaggt aggcaaacca
     6061 ccattctaag gtcatgatga actcccggtg tgtctatttt tgtgccacaa tacgctactg
     6121 tcgcagcgtt gtgtcaggca cgctaaaaaa aacgatttta cgtggtttaa gaggcagatt
     6181 acccgatgtt tcagcagcaa aaagactggg aaacaagaga aaacgcgttt gctgctttta
     6241 ccatgggacc gctgactgat ttctggcgtc agcgtgatga agcagagttt actggtgtgg
     6301 atgacattcc ggtgcgcttt gtccgttttc gcgcacagca ccatgaccgg gtggtagtca
     6361 tctgcccggg gcgtattgag agctacgtaa aatatgcgga actggcctac gacctgttcc
     6421 atttggggtt tgatgtctta atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt
     6481 tagccgatcc gcatctcggg catgttaatc gctttaatga ttatgttgat gatctggcgg
     6541 cattctggca gcaggaggtt cagcccggtc cgtggcgtaa acgctatata ctggcacatt
     6601 cgatgggcgg tgcgatctcc acattatttc tgcaacgcca tccaggtgta tgtgacgcca
     6661 ttgcgctaac tgcgccaatg tttgggatcg tgattcgtat gccgtcattt atggcacggc
     6721 agatcctcaa ctgggccgaa gcgcatccac gtttccgtga tggctatgca ataggcaccg
     6781 ggcgctggcg cgcgttgccg tttgctatca acgtactgac ccacagcaga cagcgatatc
     6841 gacgtaactt acgcttctat gctgatgacc caacgattcg cgtcggtggg ccgacctacc
     6901 attgggtacg cgaaagtatt ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg
     6961 ccacgccaac gcttctcttg caggctgaag aggaacgcgt ggtggataac cgcatgcatg
     7021 accgtttttg tgaactccgc accgccgcgg gccatcctgt cgaaggagga cggccgttgg
     7081 taattaaagg tgcttaccat gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc
     7141 tccacgccat cgttgatttt ttcaacaggc ataactcacc cagcggaaac cgctctacag
     7201 aggtttaaat ttcttatgta ccaggttgtt gcgtctgatt tagatggcac gttactttct
     7261 cccgaccata cgttatcccc ttacgccaaa gaaactctga agctgctcac cgcgcgcggc
     7321 atcaactttg tgtttgcgac cggtcgtcac cacgttgatg tggggcaaat tcgcgataat
     7381 ctggagatta agtcttacat gattacctcc aatggtgcgc gcgttcacga tctggatggt
     7441 aatctgattt ttgctcataa cctggatcgc gacattgcca gcgatctgtt tggcgtagtc
     7501 aacgacaatc cggacatcat tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc
     7561 catcgcccgg aagagatgcg cttttttaaa gaagcggtgt tccaatatgc gctgtatgag
     7621 cctggattac tggagccgga aggcgtcagc aaagtgttct tcacctgcga ttcccatgaa
     7681 caactgctgc cgctggagca ggcgattaac gctcgttggg gcgatcgcgt caacgtcagt
     7741 ttctctacct taacctgtct ggaagtgatg gcgggcggcg tttcaaaagg ccatgcgctg
     7801 gaagcggtgg cgaagaaact gggctacagc ctgaaggatt gtattgcgtt tggtgacggg
     7861 atgaacgacg ccgaaatgct gtcgatggcg gggaaaggct gcattatggg cagtgcgcac
     7921 cagcgtctga aagaccttca tcccgagctg gaagtgattg gtactaatgc cgacgacgcg
     7981 gtgccgcatt atctgcgtaa actctattta tcgtaatcgt tctttatttg gtcagttgtc
     8041 aacctgatac ttcgctacaa tggatacccg ttaatcaaag agttttccat tgtggcgcta
     8101 cttatcatca ccacgattct gtgggccttc tcctttagct tttatggcga gtaccttgcg
     8161 gggcacgtcg atagctattt tgcggtgctg gtgcgcgttg gcctggcggc actcgttttt
     8221 ctgccgtttc tgcgtacccg tggcaatagc ctgaaaacag tcggcctgta tatgctggtg
     8281 ggcgcgatgc agctcggcgt gatgtatatg ctgagtttcc gcgcttatct ctacctgacg
     8341 gtttccgaac tgctgctgtt caccgtactg acgccgctct acatcacgct gatttatgac
     8401 atcatgagta agcgccgtct gcgctgggga tatgctttta gtgccttgtt ggcggtgatt
     8461 ggtgccggga ttattcgcta tgatcaggtc accgaccatt tctggactgg cttgttgctg
     8521 gtgcaactct ccaatatcac ttttgccatc ggcatggtgg gttacaaacg cctgatggaa
     8581 actcgcccga tgccgcagca taacgccttt gcgtggttct atcttggcgc gttcctggtg
     8641 gcggtaattg catggttctt gctgggaaat acgcagaaaa tgccgcaaac cacgttgcaa
     8701 tggggcattc tggtgtttct tggcgtggtg gcttccggga ttggctactt tatgtggaac
     8761 tacggcgcga cgcaggtgga cgccggaacg ctgggcatta tgaataatat gcacgttccg
     8821 gcagggctgc tggtaaacct ggctatctgg caccaacagc cgcactggcc aacgttcatt
     8881 acaggcgcgc tggtgatcct ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc
     8941 tcttcacaaa cggcagatga tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg
     9001 taactggctg acgctgctct ccatcgcgca cggcggcgta cagtcggctc cacaagcctt
     9061 cgcccagggt tttggtcacc accagaccct ggcgctcaaa actctctact acccaatgcg
     9121 gtagcgcggc aatacccatc cgtgcggcaa ccatctgaat caacaataag gtgttatcga
     9181 cgcttttcag tgacgggctg acgcctgccg gctgaagaaa atgccgccag acatccagtc
     9241 gactacgctg taccggataa atcaacagcg tctcgctggc gagatcttcc ggtgtaattc
     9301 gcgttttcgc cgccagtgga tggtcaggtg ctaacaccag acgcacttca tagtcgaaca
     9361 tcggcgaata atgcaggcca ctgcgcggca ggatatcgga ggtcatcacc agatccagct
     9421 cgccctgttg caacgcgggc tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta
     9481 cctgcggcca gttcttatgg aaattttcta acgcgggcgt cagccactga atacagctat
     9541 ggcactcaat ggcaatgcgc agacgcgtct gctgcggttc attgcaggct tgcagtgcct
     9601 ggctgatttg cggcagcacc tggtttgcca gttgcaacag gatttctccc tgcggtgtaa
     9661 agcgtagcgg ctggctctta cgcacaaata gccggaagcc aaggcgttgt tccagatcgc
     9721 taaactggtg agacagggcg gattgcgtct gatgcaacgt cgccgcagcg gctgcgagag
     9781 agccgcagtt ccgcaacgct tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc
     9841 ttcacttcgg catgaataat ttgcgcttga ggaatataca gtaaccgcca attatggatg
     9901 tgtaaacatc tggacggcta aatactaaaa attccataag gggcatataa tgacaattct
     9961 taatcacacc ctcggtttcc ctcgcgttgg cctgcgtcgc gagctgaaaa aagcgcaaga
    10021 aagttattgg gcggggaact ccacgcgtga agaactgctg acagtagggc gtgaactgcg
    10081 tgctcgtcac tgggatcaac aaaagcaagc gggtatcgac ctgctgccgg tgggcgattt
    10141 tgcctggtac gatcatgtac tgaccaccag tttgctgctg ggtaatgttc cgccacgtca
    10201 tcagaacaaa gacggttcgg tagatatcga caccctgttc cgtattggtc gtggacgtgc
    10261 gccgactggc gaacctgcgg cggcagcgga aatgaccaaa tggtttaaca ccaactatca
    10321 ctacatggtg ccggagttcg ttaaaggcca acagttcaaa ctgacctgga cgcagctgct
    10381 ggaggaagtg gacgaggcgc tggcgctggg ccacaacgtt aagcctgtgc tgctggggcc
    10441 ggttacttac ctgtggctgg ggaaagtgaa aggtgaacag tttgaccgcc ttagcctgct
    10501 gaacgacatt ctgccggttt atcagcaagt gctggcagaa ctggcgaaac gcggcatcga
    10561 gtgggtacag attgatgaac ccgcgctggt actggaacta ccacaggcgt ggctggacgc
    10621 atacaaaccc gcttacgacg cgctccaggg acaggtgaaa ctgctgctga ccacctattt
    10681 tgaaggcgta acgccaaatc tcgacacgat tactgcgctg cctgttcagg gtctgcatgt
    10741 tgacctcgta catggtaaag atgacgttgc tgaactgcac aagcgcctgc cttctgactg
    10801 gttgctgtct gcgggtctga tcaatggtcg taacgtctgg cgcgccgatc ttaccgagaa
    10861 atatgcgcaa attaaggaca ttgtcggcaa acgtgatttg tgggtggcat cttcctgctc
    10921 gttgctgcac agccccatcg acctgagcgt ggaaacgcgt cttgatgcag aagtgaaaag
    10981 ctggtttgcc ttcgccctac aaaaatgcca tgaactggca ctgctgcgcg atgcgctgaa
    11041 cagtggtgac acggcagctc tggcagagtg gagcgccccg attcaggcac gtcgtcactc
    11101 tacccgcgta cataatccgg cggtagaaaa gcgtctggcg gcgatcaccg cccaggacag
    11161 ccagcgtgcg aatgtctatg aagtgcgtgc tgaagcccag cgtgcgcgtt ttaaactgcc
    11221 agcgtggccg accaccacga ttggttcctt cccgcaaacc acggaaattc gtaccctgcg
    11281 tctggatttc aaaaagggca atctcgacgc caacaactac cgcacgggca ttgcggaaca
    11341 tatcaagcag gccattgttg agcaggaacg tttgggactg gatgtgctgg tacatggcga
    11401 ggccgagcgt aatgacatgg tggaatactt tggcgagcac ctcgacggat ttgtctttac
    11461 gcaaaacggt tgggtacaga gctacggttc ccgctgcgtg aagccaccga ttgtcattgg
    11521 tgacattagc cgcccggcac cgattaccgt ggagtgggcg aagtatgcgc aatcgctgac
    11581 cgacaaaccg gtgaaaggga tgctgacggg gccggtgacc atactctgct ggtcgttccc
    11641 gcgtgaagat gtcagccgtg aaaccatcgc caaacagatt gcgctggcgc tgcgtgatga
    11701 agtggccgat ctggaagccg ctggaattgg catcatccag attgacgaac cggcgctgcg
    11761 cgaaggttta ccgctgcgtc gtagcgactg ggatgcgtat ctccagtggg gcgtagaggc
    11821 cttccgtatc aacgccgccg tggcgaaaga tgacacacaa atccacactc acatgtgtta
    11881 ttgcgagttc aacgacatca tggattcgat tgcggcgctg gacgcagacg tcatcaccat
    11941 cgaaacctcg cgttccgaca tggagttgct ggagtcgttt gaagagtttg attatccaaa
    12001 tgaaatcggt cctggcgtct atgacattca ctcgccaaac gtaccgagcg tggaatggat
    12061 tgaagccttg ctgaagaaag cggcaaaacg cattccggca gagcgcctgt gggtcaaccc
    12121 ggactgtggc ctgaaaacgc gcggctggcc agaaacccgc gcggcactgg cgaacatggt
    12181 gcaggcggcg cagaacttgc gtcgggggta aaatccaaac cgggtggtaa taccacccgg
    12241 tcttttctca ttacagcgac ttcttcccac catactgctt aaaccattcc agcatacgct
    12301 gccagccatc ttctgcagat gcggcatgat agctcgggcg ataatcagcg ttgaatgcat
    12361 gcccggcgtc cgggtacacg ataatctctg ctttcgcatt agcagcccgc agcgcctggc
    12421 gcatggtttc aacgctctcc tgcggaatgc tgttatcctg accaccatat aagccgagaa
    12481 tcggcgcgtt aagatcggtt gcgatatcaa caggttgttt cggtgaattc agcgacttgt
    12541 cgcccgtcag tttgccgtac cacgccactg cggcttttag ctgtggatta tgcgcggcat
    12601 acagccaggt gatacgtcca ccccagcaga atccggtgat cattaaacga tgaacatcgc
    12661 cgccgttgcg ggaagcccaa ctggcgacat gatcgagatc ggccagcacc tgcgagtcag
    12721 gcacttttgc taccagaccg ctaagcaacg tggggatatc ggcaaaatca ttcggatcgc
    12781 cttcgcggaa gtaaagttca ggtgcgatag ccagataccc ctccagcgcc agacggcgac
    12841 aaatgtcgcg gatatgttca tgcacgccaa aaatttcctg cactacaatg accactggca
    12901 gtgggccatc gctttgcttt ggtctggcat ggtaagcagg catgttatcc ccttgtgaag
    12961 ggatagaggt gaagcccgcc acaatcgcgt cgtccggggt ctgaacgatg gtcgaagcga
    13021 gaggcgatgc agcaggtgca aatccagatt gttgtgttgt tgccatggta ttctccgtac
    13081 ccttataaaa atgttgcgca atgttaacta tagtcagcat gcaacaaatc acattgcctg
    13141 aatcggctca tcttttatgc agtcctgcag aatgaagagt gatttatgtg atttgcatca
    13201 cttttggtgg gtaaatttat gcaacgcatt tgcgtcatgg tgatgagtat cacgaaaaaa
    13261 tgttaaaccc ttcggtaaag tgtctttttg cttcttctga ctaaaccgat tcacagagga
    13321 gttgtatatg tccaagtctg atgtttttca tctcggcctc actaaaaacg atttacaagg
    13381 ggctacgctt gccatcgtcc ctggcgaccc ggatcgtgtg gaaaagatcg ccgcgctgat
    13441 ggataagccg gttaagctgg catctcaccg cgaattcact acctggcgtg cagagctgga
    13501 tggtaaacct gttatcgtct gctctaccgg tatcggcggc ccgtctacct ctattgctgt
    13561 tgaagagctg gcacagctgg gcattcgcac cttcctgcgt atcggtacaa cgggcgctat
    13621 tcagccgcat attaatgtgg gtgatgtcct ggttaccacg gcgtctgtcc gtctggatgg
    13681 cgcgagcctg cacttcgcac cgctggaatt cccggctgtc gctgatttcg aatgtacgac
    13741 tgcgctggtt gaagctgcga aatccattgg cgcgacaact cacgttggcg tgacagcttc
    13801 ttctgatacc ttctacccag gtcaggaacg ttacgatact tactctggtc gcgtagttcg
    13861 tcactttaaa ggttctatgg aagagtggca ggcgatgggc gtaatgaact atgaaatgga
    13921 atctgcaacc ctgctgacca tgtgtgcaag tcagggcctg cgtgccggta tggtagcggg
    13981 tgttatcgtt aaccgcaccc agcaagagat cccgaatgct gagacgatga aacaaaccga
    14041 aagccatgcg gtgaaaatcg tggtggaagc ggcgcgtcgt ctgctgtaat tctcttctcc
    14101 tgtctgaagg ccgacgcgtt cggccttttg tatttttgcg tagcgcctcg caggaaatgc
    14161 ctttccaact ggacgtttgt acagcacaat tctattttgt gcgggtaagt tgttgcgtca
    14221 ggaggcgttg tggatttctc aatcatggtt tacgcagtta ttgcgttggt gggtgtggca
    14281 attggctggc tgtttgccag ttatcaacat gcgcagcaaa aagccgagca attagctgaa
    14341 cgtgaagaga tggtcgcgga gttaagcgcg gcaaaacaac aaattaccca aagcgagcac
    14401 tggcgtgcag agtgcgagtt actcaataac gaagtgcgca gcctgcaaag tattaacacc
    14461 tctctggagg ccgatctgcg tgaagtaacc acgcggatgg aagccgcaca gcaacatgct
    14521 gacgataaaa ttcgccagat gattaacagc gagcagcgcc tcagtgagca gtttgaaaac
    14581 ctcgccaacc gtatttttga gcacagcaat cgccgggttg atgagcaaaa ccgtcagagt
    14641 ctgaacagcc tgttgtcgcc gctacgtgaa caactggacg gtttccgccg tcaggttcag
    14701 gacagcttcg gtaaagaagc acaagaacgc cataccctga cccacgaaat tcgcaatctc
    14761 cagcaactca acgcgcaaat ggcccaggaa gcgatcaacc tgacgcgcgc gctgaaaggc
    14821 gacaataaaa cccagggcaa ctggggcgag gtagtattga cgcgggtgct ggaggcttcc
    14881 ggtctgcgtg aagggtatga atatgaaacc caggtcagca tcgaaaatga cgcccgctcg
    14941 cggatgcagc cggatgtcat cgtgcgcctg ccgcagggaa aagatgtggt gatcgacgcc
    15001 aaaatgacgc tggtcgccta tgaacgctat tttaacgccg aagacgacta cacccgcgaa
    15061 agcgcgctac aggaacatat cgcgtcggtg cgtaaccata tccgtttgct gggacgcaaa
    15121 gattatcaac agctgccggg gctgcgaact ctggattacg tgctgatgtt tattcccgtt
    15181 gaacccgctt ttttactggc gcttgaccgc cagccggagc tgatcaccga agcgttgaaa
    15241 aacaacatca tgctggttag cccgactacg ctgctggtgg cgctgcgcac tatcgccaac
    15301 ctgtggcgtt atgagcatca aagccgcaac gcccagcaaa tcgccgatcg tgccagcaag
    15361 ctgtacgaca agatgcgttt gttcatcgat gacatgtccg cgattggtca aagtctcgac
    15421 aaagcgcagg ataattatcg gcaggcaatg aaaaaactct cttcagggcg cggaaatgtg
    15481 ctggcgcagg cagaagcgtt tcgcggttta ggagtagaaa ttaaacgcga gattaatccg
    15541 gatttggctg aacaggcggt gagccaggat gaagagtatc gacttcggtc ggttccggag
    15601 cagccgaatg atgaagctta tcaacgcgat gatgaatata atcagcagtc gcgctagccc
    15661 attgggagta gttaagccgg gtagaaatct agggcatcga cgcccaatct gttacacttc
    15721 tggaacaatt ttttgatgag caggcattga gatggtggat aagtcacaag aaacgacgca
    15781 ctttggtttt cagaccgtcg cgaaggaaca aaaagcggat atggtcgccc acgttttcca
    15841 ttccgtggca tcaaaatacg atgtcatgaa tgatttgatg tcatttggta ttcatcgttt
    15901 gtggaagcga ttcacgattg attgcagcgg cgtacgccgt gggcagaccg tgctggatct
    15961 ggctggtggc accggcgacc tgacagcgaa attctcccgc ctggtcggag aaactggcaa
    16021 agtggtcctt gctgatatca atgaatccat gctcaaaatg ggccgcgaga agctgcgtaa
    16081 tatcggtgtg attggcaacg ttgagtatgt tcaggcgaac gctgaggcgc tgccgttccc
    16141 ggataacacc tttgattgca tcaccatttc gtttggtctg cgtaacgtca ccgacaaaga
    16201 taaagcactg cgttcaatgt atcgcgtgct gaaacccggc ggccgcctgc tggtgcttga
    16261 gttctcgaag ccaattatcg agccgctgag caaagcctat gatgcatact ccttccatgt
    16321 gctgccgcgt attggctcac tggtcgcgaa cgacgccgac agctaccgtt atctggcaga
    16381 atccatccgt atgcatcccg atcaggatac cctgaaagcc atgatgcagg atgccggatt
    16441 cgaaagtgtc gactactaca atctgacggc aggggttgtg gcgctgcatc gtggttataa
    16501 gttctgacag gagaccggaa atgcctttta aacctttagt gacggcagga attgaaagtc
    16561 tgctcaacac cttcctgtat cgctcacccg cgctgaaaac ggcccgctcg cgtctgctgg
    16621 gtaaagtatt gcgcgtggag gtaaaaggct tttcgacgtc attgattctg gtgttcagcg
    16681 aacgccaggt tgatgtactg ggcgaatggg caggcgatgc tgactgcacc gttatcgcct
    16741 acgccagtgt gttgccgaaa cttcgcgatc gccagcagct taccgcactg attcgcagtg
    16801 gtgagctgga agtgcagggc gatattcagg tggtgcaaaa cttcgttgcg ctggcagatc
    16861 tggcagagtt cgaccctgcg gaactgctgg tcccttatac cggtgatatc gccgctgaag
    16921 gaatcagcaa agccatgcgc ggaggcgcaa agttcctgca tcacggcatt aagcgccagc
    16981 aacgttatgt ggcggaagcc attactgaag agtggcgtat ggcacccggt ccgcttgaag
    17041 tggcctggtt tgcggaagag acggctgccg tcgagcgtgc tgttgatgcc ctgaccaaac
    17101 ggctggaaaa actggaggct aaatgacgcc aggtgaagta cggcgcctat atttcatcat
    17161 tcgcactttt ttaagctacg gacttgatga actgatcccc aaaatgcgta tcaccctgcc
    17221 gctacggcta tggcgatact cattattctg gatgccaaat cggcataaag acaaactttt
    17281 aggtgagcga ctacgactgg ccctgcaaga actggggccg gtttggatca agttcgggca
    17341 aatgttatca acccgccgcg atctttttcc accgcatatt gccgatcagc tggcgttatt
    17401 gcaggacaaa gttgctccgt ttgatggcaa gctggcgaag cagcagattg aagctgcaat
    17461 gggcggcttg ccggtagaag cgtggtttga cgattttgaa atcaagccgc tggcttctgc
    17521 ttctatcgcc caggttcata ccgcgcgatt gaaatcgaat ggtaaagagg tggtgattaa
    17581 agtcatccgc ccggatattt tgccggttat taaagcggat ctgaaactta tctaccgtct
    17641 ggctcgctgg gtgccgcgtt tgctgccgga tggtcgccgt ctgcgcccaa ccgaagtggt
    17701 gcgcgagtac gaaaagacat tgattgatga actgaatttg ctgcgggaat ctgccaacgc
    17761 cattcagctt cggcgcaatt ttgaagacag cccgatgctc tacatcccgg aagtttaccc
    17821 tgactattgt agtgaaggga tgatggtgat ggagcgcatt tacggcattc cggtgtctga
    17881 tgttgcggcg ctggagaaaa acggcactaa catgaaattg ctggcggaac gcggcgtgca
    17941 ggtgttcttc actcaggtct ttcgcgacag ctttttccat gccgatatgc accctggcaa
    18001 catcttcgta agctatgaac acccggaaaa cccgaaatat atcggcattg attgcgggat
    18061 tgttggctcg ctaaacaaag aagataaacg ctatctggca gaaaacttta tcgccttctt
    18121 taatcgcgac tatcgcaaag tggcagagct acacgtcgat tctggctggg tgccaccaga
    18181 taccaacgtt gaagagttcg aatttgccat tcgtacggtc tgtgaaccta tctttgagaa
    18241 accgctggcc gaaatttcgt ttggacatgt actgttaaat ctgtttaata cggcgcgtcg
    18301 cttcaatatg gaagtgcagc cgcaactggt gttactccag aaaaccctgc tctacgtcga
    18361 aggggtagga cgccagcttt atccgcaact cgatttatgg aaaacggcga agcctttcct
    18421 ggagtcgtgg attaaagatc aggtcggtat tcctgcgctg gtgagagcat ttaaagaaaa
    18481 agcgccgttc tgggtcgaaa aaatgccaga actgcctgaa ctggtttacg acagtttgcg
    18541 ccagggcaag tatttacagc acagtgttga taagattgcc cgcgagcttc agtcaaatca
    18601 tgtacgtcag ggacaatcgc gttattttct cggaattggc gctacgttag tattaagtgg
    18661 cacattcttg ttggtcagcc gacctgaatg ggggctgatg cccggctggt taatggcagg
    18721 tggtctgatc gcctggtttg tcggttggcg caaaacacgc tgattttttc atcgctcaag
    18781 gcgggccgtg taacgtataa tgcggccttg tttaatcatc atctaccaca gaggaacatg
    18841 tatgggtggt atcagtattt ggcagttatt gattattgcc gtcatcgttg tactgctttt
    18901 tggcaccaaa aagctcggct ccatcggttc cgatcttggt gcgtcgatca aaggctttaa
    18961 aaaagcaatg agcgatgatg aaccaaagca ggataaaacc agtcaggatg ctgattttac
    19021 tgcgaaaact atcgccgata agcaggcgga tacgaatcag gaacaggcta aaacagaaga
    19081 cgcgaagcgc cacgataaag agcaggtgta atccgtgttt gatatcggtt ttagcgaact
    19141 gctattggtg ttcatcatcg gcctcgtcgt tctggggccg caacgactgc ctgtggcggt
    19201 aaaaacggta gcgggctgga ttcgcgcgtt gcgttcactg gcgacaacgg tgcagaacga
    19261 actgacccag gagttaaaac tccaggagtt tcaggacagt ctgaaaaagg ttgaaaaggc
    19321 gagcctcact aacctgacac ccgaactgaa agcgtcgatg gatgaactac gccaggccgc
    19381 ggagtcgatg aagcgttcct acgttgcaaa cgatcctgaa aaggcgagcg atgaagcgca
    19441 caccatccat aacccggtgg tgaaagataa tgaagctgcg catgagggcg taacgcctgc
    19501 cgctgcacaa acgcaggcca gttcgccgga acagaagcca gaaaccacgc cagagccggt
    19561 ggtaaaacct gctgcggacg ctgaaccgaa aaccgctgca ccttcccctt cgtcgagtga
    19621 taaaccgtaa acatgtctgt agaagatact caaccgctta tcacgcatct gattgagctg
    19681 cgtaagcgtc tgctgaactg cattatcgcg gtgatcgtga tattcctgtg tctggtctat
    19741 ttcgccaatg acatctatca cctggtatcc gcgccattga tcaagcagtt gccgcaaggt
    19801 tcaacgatga tcgccaccga cgtggcctcg ccgttcttta cgccgatcaa gctgaccttt
    19861 atggtgtcgc tgattctgtc agcgccggtg attctctatc aggtgtgggc atttatcgcc
    19921 ccagcgctgt ataagcatga acgtcgcctg gtggtgccgc tgctggtttc cagctctctg
    19981 ctgttttata tcggcatggc gttcgcctac tttgtggtct ttccgctggc atttggcttc
    20041 cttgccaata ccgcgccgga aggggtgcag gtatccaccg acatcgccag ctatttaagc
    20101 ttcgttatgg cgctgtttat ggcgtttggt gtctcctttg acgtgccggt ggcaattgtg
    20161 ctgctgtgct ggatggggat tacctcgcca gaagacttac gcaaaaaacg cccgtatgtg
    20221 ctggttggtg cattcgttgt cgggatgttg ctgacgccgc cggatgtctt ctcgcaaacg
    20281 ctgttggcga tcccgatgta ctgtctgttt gaaatcggtg tcttcttctc acgcttttac
    20341 gttggtaaag ggagaaatcg ggaagaggaa aacgacgctg aagcagaaag cgaaaaaact
    20401 gaagaataaa ttcaaccgcc cgtcagggcg gttgtcatat ggagtacagg atgtttgata
    20461 tcggcgttaa tttgaccagt tcgcaatttg cgaaagaccg tgatgatgtt gtagcgcgcg
    20521 cttttgacgc gggagttaat gggctactca tcaccggcac taacctgcgt gaaagccagc
    20581 aggcgcaaaa gctggcgcgt cagtattcgt cctgttggtc aacggcgggc gtacatcctc
    20641 acgacagcag ccagtggcaa gctgcgactg aagaagcgat tattgagctg gccgcgcagc
    20701 cagaagtggt ggcgattggt gaatgtggtc tcgactttaa ccgcaacttt tcgacgccgg
    20761 aagagcagga acgcgctttt gttgcccagc tacgcattgc cgcagattta aacatgccgg
    20821 tatttatgca ctgtcgcgat gcccacgagc ggtttatgac attgctggag ccgtggctgg
    20881 ataaactgcc tggtgcggtt cttcattgct ttaccggcac acgcgaagag atgcaggcgt
    20941 gcgtggcgca tggaatttat atcggcatta ccggttgggt ttgcgatgaa cgacgcggac
    21001 tggagctgcg ggaacttttg ccgttgattc cggcggaaaa attactgatc gaaactgatg
    21061 cgccgtatct gctccctcgc gatctcacgc caaagccatc atcccggcgc aacgagccag
    21121 cccatctgcc ccatattttg caacgtattg cgcactggcg tggagaagat gccgcatggc
    21181 tggctgccac cacggatgct aatgtcaaaa cactgtttgg gattgcgttt tagagtttgc
    21241 ggaactcggt gttcttcaca ctgtgcttaa tctctttatt aataagatta agcaatagca
    21301 tggagcgagc ctcaccatcg ggttcggtga aaatggcctg aaagccttcg aacgcgcctt
    21361 cggtaataat caccttatca cccggataag gggttgccgg atcgacaatg tctttcggtt
    21421 tatataccga tagctgatga atcaccgccg atgggactat cgctggcgac gcgccaaagc
    21481 gcacgaagtg gctgacaccg cgggtcgcgt tgatagtcgt ggtatgaatc acttctggat
    21541 caaattcgac aaacaggtag ttgggaaaca atggctcact gactgcagta cgttttccac
    21601 gcacgatttt ttccagggtg atcatcggtg ccaggcaatt cacagcctgt ctttcgaggt
    21661 gttcctgggc acgttgaagt tgcccgcgct tgcagtacag taaataccag gattgcataa
    21721 tgactcttat ccgtttaatc ggggcgcaag gatagcaaaa gctttacgct aagttaatta
    21781 tattccccgg tttgcgttat accgtcagag ttcacgctaa tttaacaaat ttacagcatc
    21841 gcaaagatga acgccgtata atgggcgtag attaagaggc tacaatggac gccatgaaat
    21901 ataacgattt acgcgacttc ctgacgctgc ttgaacagca gggtgagcta aaacgtatca
    21961 cgctcccggt ggatccgcat ctggaaatca ctgaaattgc tgaccgcact ttgcgtgccg
    22021 gtgggcctgc gctgttgttc gaaaacccta aaggctactc aatgccggtg ctgtgcaacc
    22081 tgttcggtac gccaaagcgc gtggcgatgg gcatggggca ggaagatgtt tcggcgctgc
    22141 gtgaagttgg taaattattg gcgtttctga aagagccgga gccgccaaaa ggtttccgcg
    22201 acctgtttga taaactgccg cagtttaagc aagtattgaa catgccgaca aagcgactgc
    22261 gtggtgcgcc ctgccaacaa aaaatcgtct ctggcgatga cgtcgatctc aatcgcattc
    22321 ccattatgac ctgctggccg gaagatgccg cgccgctgat tacctggggg ctgaccgtaa
    22381 cgcgcggccc gcataaagag cggcagaatc tgggcattta tcgtcagcag ctgattggta
    22441 aaaacaaact gattatgcgc tggctgtcgc atcgcggcgg cgcgctggat tatcaggagt
    22501 ggtgtgcggc gcatccgggc gaacgtttcc cggtttctgt ggcgctgggt gccgatcccg
    22561 ccacgattct cggtgcagtc acccccgttc cggatacact ttcagagtat gcgtttgccg
    22621 gattgctgcg cggtaccaag accgaagtgg tgaagtgtat ctccaatgac cttgaagtgc
    22681 ccgccagtgc ggagattgtg ctggaagggt atatcgaaca aggtgaaacg gcgccggaag
    22741 ggccgtatgg cgaccacacc ggttactata acgaagtcga tagtttcccg gtatttaccg
    22801 tgacgcatat tacccagcgt gaagatgcga tttaccactc cacctatact ggtcgtccgc
    22861 cggatgaacc cgcggtgctg ggtgtcgcac tgaacgaagt gtttgtgccg attctgcaaa
    22921 aacagttccc ggaaattgtc gatttttacc tgccgccgga aggctgttct tatcgtctgg
    22981 cggtagtgac gatcaaaaaa cagtacgccg gacacgcgaa gcgcgtcatg atgggcgtct
    23041 ggtcgttctt acgccagttt atgtacacta aatttgtgat cgtttgcgat aatgacgtca
    23101 acgcacgcga ctggaacgat gtgatttggg cgattaccac ccgtatggac ccggcgcggg
    23161 atactgttct ggtagaaaat acgcctattg attatctgga ttttgcctcg cctgtctccg
    23221 ggctgggttc aaaaatgggg ctggatgcca cgaataaatg gccgggggaa acccagcgtg
    23281 aatggggacg tcccatcaaa aaagatccag atgttgtcgc gcatattgac gccatctggg
    23341 atgaactggc tatttttaac aacggtaaaa gcgcctgatg cgcgtttgtt ttgccctatt
    23401 tatcgatccg acagagaaag cgcatgacaa ccttaagctg taaagtgacc tcggtagaag
    23461 ctatcacgga taccgtatat cgtgtccgca tcgtgccaga cgcggccttt tcttttcgtg
    23521 ctggtcagta tttgatggta gtgatggatg agcgcgacaa acgtccgttc tcaatggcct
    23581 cgacgccgga tgaaaaaggg tttatcgagc tgcatattgg cgcttctgaa atcaaccttt
    23641 acgcgaaagc agtcatggac cgcatcctca aagatcatca aatcgtggtc gatattcccc
    23701 acggagaagc gtggctgcgc gatgatgaag agcgtccgat gattttgatt gcgggcggca
    23761 ccgggttctc ttatgcccgc tcgattttgc tgacagcgtt ggcgcgtaac ccaaaccgtg
    23821 atatcaccat ttactggggc gggcgtgaag agcagcatct gtatgatctc tgcgagcttg
    23881 aggcgctttc gttaaagcac cctggtctgc aagtggtgcc ggtggttgaa caaccggaag
    23941 caggctggcg tgggcgtact ggtaccgtgt taacggcggt attgcaggat catggtacgc
    24001 tggcagagca tgatatctat attgccggac gttttgagat ggcgaaaatt gcccgtgacc
    24061 tgttttgcag tgagcgtaat gcgcgggaag atcgcctgtt tggcgatgcg tttgcattta
    24121 tctgagatat aaaaaaaccc gcccctgaca ggcgggaaga acggcaacta aaactttatt
    24181 cagtggcatt tagatctatg acgtatctgg caaaccatgc ccgatgcgac gctgtcgcgt
    24241 cttatcgtgc ctacaaatag tccgaaccgt aggccggata aggcgtttac gccgcatccg
    24301 gcaattggtg cacgatgcct gatgcgacgc tggcgcgtct tatcaggcct acattggtgc
    24361 cggatctgta gaccggataa ggcgttcacg ccgcatccgg caagtggtta aacccgctca
    24421 aacaccgtcg caataccctg acccagaccg atacacatcg tcgccagacc aaactgaacg
    24481 tctttgcgtt ccatcagatt cagcagcgtg gtgctgatac gcgcaccgga acagcccagc
    24541 ggatgaccca gcgcgatcgc accaccattg aggttgatct tctcgtcaat ctgctccatt
    24601 agcccgagat ctttaatgca cggcaggatc tgcgcggcaa aggcttcgtt catctcaaac
    24661 acgccgatat cgctggcaga aagccccgct tttttcagcg ccagcttcga ggccggaacc
    24721 gggccgtaac ccataatcga tgggtcacaa ccaacgaccg ccatcgaacg cacacgagcg
    24781 cgcggcttaa gacctaattc atgggcgcgg ctttcactca tcaccagcat ggcagctgcg
    24841 ccatcggaaa gtgcagaaga tgtgcccgcc gttaccgtac cgtttactgg atcaaacgcc
    24901 ggacgcagcg tggcgagggc ttccacggtg gtttccgggc gaatcacttc gtcgtaatta
    24961 aactgcttca ggacgccgtc ggcatcgtga ccaccagttg ggatgatttc atttttaaat
    25021 gcggccgact gcgtggcggc ccaggcgcgg gcgtgtgacc gcgcggcaaa ggcatcctgc
    25081 atttcacggc tgataccgtg catacgcgcc agcatttctg ctgttaagcc catcatgccc
    25141 gccgctttgg cgacattgcg gctcaggccg gggtgaaaat cgacgccgtg actcatcggc
    25201 acatggccca tatgctccac gccgccaacc agacatgcct gcgcatcgcc ggtcatgatc
    25261 attcgtgctg cgtcatgcag tgcctgcatg gatgaaccac acaagcgatt gacggtaacc
    25321 gccgggacag agtgcggcac ttctgccagc agcgccgcgt tacgggcgat gttaaaaccc
    25381 tgctccagcg tctgctgcac acaaccccag taaatatcgt cgagggccgc cgcttccagc
    25441 gccgggttac gcgccagcag gctacgcatt aaatgagcgg agagatcttc cgcacgcacg
    25501 ttacgaaaag caccgccctt cgaacggccc atcggggtgc gaattgcatc gacaatgaca
    25561 acctgttcca ttgtgactcc ttaagccgtt ttcaggtcgc caaccggacg ggctggctca
    25621 accgggggat agtacggttc gttatgacgt gctttattac gcagaccttc cggcacttcg
    25681 tacagcgggc cgaggtgctg atattgctgt gccatatcga ggtattttgc gctaccgagg
    25741 gtgtccagcc agcggaacgc gccgccgtgg aacggaggga agcccaggcc gtagaccagc
    25801 gccatatccg cttccgccgg agtggcgata atgccttcct ccagacagcg caccacttcg
    25861 ttgaccatcg ggatcatcat gcgggcgata atctcttctt cgctgaaatc gcgcttcggc
    25921 tggctcactt ctgccagcag gtcttcaacg gcggcgtctt cttctttctt cggcttacct
    25981 ttgctgtctt ctttataacg ccagaaaccg aggccgttct tctgaccaaa gcggttggca
    26041 tcaaacagcg cgtcgatggc atcgcggtaa tctttctgca tccgctgcgg gaagcctgct
    26101 gccatgacag cctgagcgtg atgcgcggta tcaatgccca cgacatccag cagatatgcc
    26161 ggacccatag gccagccaaa ctgtttttcc atcactttgt cgatcttgcg gaaatccgcg
    26221 ccgtcgcgca gcagctggct gaaaccggcg aaatacggga acagcacgcg gttaacaaag
    26281 aagccggggc agtcgttaac cacaatcggc gtcttgccca tcttgctcgc ccaggcgaca
    26341 actttcgcga tggtttcgtc ggagcttttc tcgccgcgaa taatttctac caacggcatc
    26401 cggtgtaccg ggttaaaaaa gtgcattccg cagaagtttt ccgggcgttc cagcgcgttg
    26461 gccagttcgc tgataggaat ggttgaagtg ttagacgcca gcacggtatc cgggcgtact
    26521 ttttgttcgg tttctgccag tacggctttt ttcactttcg ggttttcgac aaccgcttct
    26581 accacaacat ccacgcggtc aaacccggcg tagtcgagcg ttgggtggat tgtggagatc
    26641 acgccagcca gtttcagacc atcgatcttg ccgcgctcaa gctgcttgtt cagcagtttc
    26701 gcggcttcgg tcatgccgag ggttaacgac ttgtcgttga tatctttcat gacaaccggc
    26761 acgcctttcc acgcagactg gtaagcgatg ccgccgccca taatgcctgc acccagcacc
    26821 gcggcctgtt tcggggtttc aacgtctttg gtgagtttct tcgctttgcc ttttacatat
    26881 tgatcgttaa ggaaaatgcc gaccagtgcg cgggcttcgt tggtatgcgc cagcgggaca
    26941 aaacttttgt tttccaggtt taaggcttct tcacgaccaa aacgggccgc agcttcaatg
    27001 gtttttactg cggtgatggg ggccggataa tgtttccccg ctgtttgtgc gaccatccct
    27061 ttagcgatgg tgaagctcat ggtggcttca atcttgctca gttttagtgg ttccagcttc
    27121 ggctgacgtt ttgctttcca gtcgaggtcg ccgttaatgg cctggcgtaa aaccgccttt
    27181 gcgccttcaa ccagtttttc tgctttgact acgccatcca ccagaccgat tttcagcgcc
    27241 tgatccgcgc cgacatcttt accggcggca atgatttcca gcgcactgtc agcgcccagc
    27301 atacgtggca tacgtacaga accgccaaag cccggcatga tgcccagttt ggtttccggc
    27361 agaccgatgc gcagatccgg cgtcgccaga cgataatcgg tcgccagcac gcattcgcag
    27421 ccaccgccca gcgcatagcc gttgacggca gcaatggtcg gcaccggcag atcttccagg
    27481 cgattaaaca cgctattggc aaagtgcagc cactgactta actgttcttc aggaacgagg
    27541 aacagggaca aaaattcggt gatatcagca ccgacgataa aggctgcttt gttcgaacgc
    27601 agcagcagcc cttttagatc tgattgctgt tccagcacgc cgatggcctc gccgaggctg
    27661 gcgacggtcg cagtgtcgag tttattaact gaacctgggg catcaaatac cagttcggca
    27721 atgccatctt ccagccagtc aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc
    27781 tgaatccgca aggtgatctg gtcgtaccag atgagtcgaa gtgtgtattt tgtgttaaaa
    27841 atatgcaaac aaaagattaa agaaatgccg atctgatcac gctcggcaga aatcacgctc
    27901 tggatgaacg atgtgctaag atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa
    27961 tggaatcact ggcctcgctc tataaaaatc atatagctac cttacaagaa cggactcgcg
    28021 atgcgctggc gcgcttcaag ctggatgcgt tacttattca ctccggcgag ctgttcaatg
    28081 tttttctcga cgatcatccc tatccgttta aagtgaaccc gcaattcaaa gcgtgggtgc
    28141 cggtaactca ggtgccaaac tgctggttgc tggtggatgg cgtgaacaag ccgaaactgt
    28201 ggttttatct gccggttgat tactggcaca acgtcgaacc gctgccgatc tccttctgga
    28261 ctgaagatgt ggaagtgatc gcgctgccga aagccgatgg cattggtagc ctgctgcctg
    28321 ctgcgcgcgg caatatcggt tatatcggtc cggtgccgga gcgtgcgctg caactgggta
    28381 ttgaggccag caacatcaac ccgaaagggg ttatcgacta cctgcattac tatcgctcct
    28441 tcaaaaccga gtacgaactg gcctgtatgc gtgaagcgca gaaaatggcg gtcaacggtc
    28501 atcgtgcggc agaagaagcg ttccgttctg gcatgagcga gttcgatatc aacattgcct
    28561 atctgaccgc gaccggtcat cgtgataccg acgtacctta cagcaacatt gtggcgctta
    28621 acgaacacgc tgcggtgctg cattacacca aactggatca tcaggcgccg gaagagatgc
    28681 gcagcttcct gctggatgcc ggggccgaat ataacggcta tgcggctgac ctgactcgta
    28741 cctggtcggc aaaaagcgac aacgactacg cacagctggt gaaagacgta aatgatgaac
    28801 aactggcgct gatcgccacc atgaaagcag gcgttagcta tgtggattac cacatccagt
    28861 tccatcagcg catcgccaaa ctgctgcgta aacatcaaat catcaccgat atgagtgaag
    28921 aagcgatggt cgaaaacgat ctcaccggac cgtttatgcc gcacggtatc ggtcatccgc
    28981 tgggcctgca ggtgcatgac gtagccggtt ttatgcagga tgatagcggt acacacctcg
    29041 cggcaccggc aaaatatccg tacctgcgct gcacccgtat tctccagccg ggcatggtgt
    29101 taaccatcga accgggtatc tacttcattg aatcgctact ggcaccgtgg cgtgaagggc
    29161 agttcagcaa gcacttcaac tggcagaaaa ttgaagcact gaaaccgttc ggcggcattc
    29221 gtatcgaaga caacgtggtg atccacgaaa acaacgtgga aaacatgacc cgggatctga
    29281 aactggcgtg atggaaagct ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat
    29341 caaaaagagc cgcttcataa cactgttggc gcataccgat ggcgttgagg cggcgaaagc
    29401 gtttgttgaa tcggtgcggg cagaacaccc cgatgcccgc caccattgcg tggcgtgggt
    29461 ggcgggtgcg ccggatgatt ctcaacagct gggtttctct gacgacgggg agccggcagg
    29521 aacggcaggt aaaccgatgc tcgcccagct aatgggcagc ggcgtcgggg aaattaccgc
    29581 tgtggtagtg cgctactacg gtggcatatt gcttggcacc ggtgggttag tgaaagcgta
    29641 tggcggcggc gtgaatcagg cgctgcgcca gctaacgacc caacgcaaga cgccattaac
    29701 cgaatatact ttgcaatgtg aatatagtca gttaaccggc attgaagcgt tgctggggca
    29761 gtgtgacggc aaaattatca acagtgatta tcaggcattc gttctgctgc gggtggcgct
    29821 tccggcggcg aaagtggctg aattttccgc aaagctggcg gattttagcc gtggttcatt
    29881 gcaattgtta gcgattgaag aataatcccc acttcgtttt gcagaactaa ggaagcggca
    29941 gagatgcatt ttcgcgccat tacccgaatc gttgggctac tggtcatctt attttcaggg
    30001 accatgatta tccctgggct ggtagcactc atctaccggg atggagcggg ccgcgctttt
    30061 acccagacct tttttgtcgc cctcgccatt ggttctatgc tgtggtggcc gaaccgtaaa
    30121 gagaagggcg aactgaaatc ccgtgagggg tttctgatag tggtgctgtt ctggaccgtg
    30181 ctgggtagtg tcggtgcgct cccttttatc ttctcggaaa gcccgaacct cacgattacc
    30241 gatgcgtttt ttgaatcttt ctctggcctg accaccaccg gggccactac gctggtgggg
    30301 ctggattcgc tccctcatgc catcctcttt tatcgccaga tgctgcaatg gtttggcggt
    30361 atggggatca tcgtgttagc ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag
    30421 ctctatcgcg cagaaatgcc cggcccgctg aaagataaca aaatgcgccc gcgaattgcg
    30481 gaaacggcga aaaccctgtg gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg
    30541 ttgtggtttg ccggaatgga tgcctttgat gccatcggcc atagctttgc gactatcgct
    30601 attggcggct tctcgacaca tgatgccagt atcggttatt tcgacagccc gactattaac
    30661 actatcattg ctatcttcct gctgatctcc ggctgtaact acggtctgca cttttcactg
    30721 ttaagtgggc gtagtctgaa ggtttattgg cgcgatccgg aatttcgcat gtttatcggc
    30781 gtacagttta cgctggtggt tatttgtaca ctcgtactgt ggtttcacaa tgtctacagt
    30841 tcggcgctga tgacaattaa ccaggcgttt ttccaggtgg tgtcgatggc gacaaccgcc
    30901 gggtttacga ctgacagcat tgcccgctgg ccgctctttt tgccggtact gcttttatgt
    30961 tcagctttta tcggcggttg tgccgggtca acgggcggtg gcctgaaagt gatccgcatc
    31021 ctgctgctgt ttaagcaggg gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc
    31081 tatagcatta agctggggaa tcgcgcactg ccggaacgta tcctcgaagc cgtttgggga
    31141 tttttctccg cctatgcatt ggtgtttatt gtcagtatgc tggcgattat cgccacgggc
    31201 gtggatgact tttctgcttt tgcctcggtt gttgcgacat tgaataacct ggggccgggg
    31261 cttggcgtgg ttgctgataa ctttaccagt atgaacccgg tggctaaatg gatcctgatt
    31321 gccaacatgc tgtttggtcg tctcgaggtc tttacattgc tggtgctctt taccccgact
    31381 ttctggcgtg aatgatggag taatacgtga aaacattaat tcttttctca acaagggacg
    31441 gacaaacgcg cgagattgcc tcctacttgg cttcggaact gaaagaactg gggatccagg
    31501 cggatgtcgc caatgtgcac cgcattgaag aaccacagtg ggaaaactac gaccgtgtgg
    31561 tcattggtgc ttctattcgc tatggtcact accactctgc gttccaggaa tttgtcaaaa
    31621 agcatgcgac gcggctgaat tcgatgccga gcgcctttta ctccgtaaac ctggtggcgc
    31681 gtaaaccgga gaagcgcact ccacagacca acagctacgc gcggaagttt ctgatgaact
    31741 cgcaatggcg tcccgatcgc tgcgcggtca ttgccggggc gctgcgttac ccacgttatc
    31801 gctggtacga ccgttttatg atcaagctaa ttatgaagat gtcaggcggt gaaacggata
    31861 cgcgtaaaga agttgtctat accgattggg agcaggtggc gaatttcgcc cgagaaatcg
    31921 cccatttaac cgacaaaccg acgctgaaat aagcataaag aataaaaaat gcgcggtc
//
LOCUS       DAOFQD010000053        31611 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.053, whole genome shotgun sequence.
ACCESSION   DAOFQD010000053 DAOFQD010000000
VERSION     DAOFQD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.053"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            175..714
                     /locus_tag="QBF46_002394"
     CDS             175..714
                     /locus_tag="QBF46_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDQ6674569.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            1013..1897
                     /gene="tsx"
                     /locus_tag="QBF46_002395"
     CDS             1013..1897
                     /gene="tsx"
                     /locus_tag="QBF46_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDQ6674570.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(2074..2421)
                     /locus_tag="QBF46_002396"
     CDS             complement(2074..2421)
                     /locus_tag="QBF46_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDQ6674571.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(2550..3521)
                     /gene="secF"
                     /locus_tag="QBF46_002397"
     CDS             complement(2550..3521)
                     /gene="secF"
                     /locus_tag="QBF46_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDQ6674572.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(3532..5379)
                     /gene="secD"
                     /locus_tag="QBF46_002398"
     CDS             complement(3532..5379)
                     /gene="secD"
                     /locus_tag="QBF46_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDQ6674573.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(5407..5739)
                     /gene="yajC"
                     /locus_tag="QBF46_002399"
     CDS             complement(5407..5739)
                     /gene="yajC"
                     /locus_tag="QBF46_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDQ6674574.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(5762..6889)
                     /gene="tgt"
                     /locus_tag="QBF46_002400"
     CDS             complement(5762..6889)
                     /gene="tgt"
                     /locus_tag="QBF46_002400"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDQ6674575.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(6945..8015)
                     /gene="queA"
                     /locus_tag="QBF46_002401"
     CDS             complement(6945..8015)
                     /gene="queA"
                     /locus_tag="QBF46_002401"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDQ6674576.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            8108..8689
                     /gene="acpH"
                     /locus_tag="QBF46_002402"
     CDS             8108..8689
                     /gene="acpH"
                     /locus_tag="QBF46_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDQ6674577.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASRKAL"
     gene            complement(8694..10511)
                     /gene="malZ"
                     /locus_tag="QBF46_002403"
     CDS             complement(8694..10511)
                     /gene="malZ"
                     /locus_tag="QBF46_002403"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDQ6674578.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(10667..12040)
                     /gene="proY"
                     /locus_tag="QBF46_002404"
     CDS             complement(10667..12040)
                     /gene="proY"
                     /locus_tag="QBF46_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDQ6674579.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEDVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(12116..13435)
                     /gene="brnQ"
                     /locus_tag="QBF46_002405"
     CDS             complement(12116..13435)
                     /gene="brnQ"
                     /locus_tag="QBF46_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDQ6674580.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(13842..15137)
                     /gene="phoR"
                     /locus_tag="QBF46_002406"
     CDS             complement(13842..15137)
                     /gene="phoR"
                     /locus_tag="QBF46_002406"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDQ6674581.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMNEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(15195..15884)
                     /gene="phoB"
                     /locus_tag="QBF46_002407"
     CDS             complement(15195..15884)
                     /gene="phoB"
                     /locus_tag="QBF46_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDQ6674582.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            16074..17276
                     /gene="sbcD"
                     /locus_tag="QBF46_002408"
     CDS             16074..17276
                     /gene="sbcD"
                     /locus_tag="QBF46_002408"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDQ6674583.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            17273..20416
                     /gene="sbcC"
                     /locus_tag="QBF46_002409"
     CDS             17273..20416
                     /gene="sbcC"
                     /locus_tag="QBF46_002409"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDQ6674584.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDKHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            20542..21726
                     /gene="araJ"
                     /locus_tag="QBF46_002410"
     CDS             20542..21726
                     /gene="araJ"
                     /locus_tag="QBF46_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDQ6674585.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(21869..22777)
                     /gene="mak"
                     /locus_tag="QBF46_002411"
     CDS             complement(21869..22777)
                     /gene="mak"
                     /locus_tag="QBF46_002411"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDQ6674586.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQHGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            22902..23813
                     /gene="rdgC"
                     /locus_tag="QBF46_002412"
     CDS             22902..23813
                     /gene="rdgC"
                     /locus_tag="QBF46_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDQ6674587.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(23891..23974)
                     /locus_tag="QBF46_002413"
     CDS             complement(23891..23974)
                     /locus_tag="QBF46_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674588.1"
                     /translation="MTQRPWSKLQRKTHNIAALKIIARRSE"
     gene            complement(23971..24312)
                     /locus_tag="QBF46_002414"
                     /pseudo
     CDS             complement(23971..24312)
                     /locus_tag="QBF46_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(24460..24744)
                     /gene="ppnP"
                     /locus_tag="QBF46_002415"
     CDS             complement(24460..24744)
                     /gene="ppnP"
                     /locus_tag="QBF46_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDQ6674589.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(24816..25493)
                     /locus_tag="QBF46_002416"
     CDS             complement(24816..25493)
                     /locus_tag="QBF46_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDQ6674590.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(25751..25942)
                     /gene="yaiA"
                     /locus_tag="QBF46_002417"
     CDS             complement(25751..25942)
                     /gene="yaiA"
                     /locus_tag="QBF46_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDQ6674591.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(25992..26516)
                     /gene="aroL"
                     /locus_tag="QBF46_002418"
     CDS             complement(25992..26516)
                     /gene="aroL"
                     /locus_tag="QBF46_002418"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDQ6674592.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(26693..27157)
                     /locus_tag="QBF46_002419"
     CDS             complement(26693..27157)
                     /locus_tag="QBF46_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDQ6674593.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAAINPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRGQM"
     gene            27277..28086
                     /gene="proC"
                     /locus_tag="QBF46_002420"
     CDS             27277..28086
                     /gene="proC"
                     /locus_tag="QBF46_002420"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDQ6674594.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(28103..29218)
                     /gene="adrA"
                     /locus_tag="QBF46_002421"
                     /gene_synonym="dgcC"
     CDS             complement(28103..29218)
                     /gene="adrA"
                     /locus_tag="QBF46_002421"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDQ6674595.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(29320..29640)
                     /gene="psiF"
                     /locus_tag="QBF46_002422"
     CDS             complement(29320..29640)
                     /gene="psiF"
                     /locus_tag="QBF46_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDQ6674596.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKNLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(29759..31174)
                     /gene="phoA"
                     /locus_tag="QBF46_002423"
     CDS             complement(29759..31174)
                     /gene="phoA"
                     /locus_tag="QBF46_002423"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDQ6674597.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(31275..31535)
                     /gene="iraP"
                     /locus_tag="QBF46_002424"
     CDS             complement(31275..31535)
                     /gene="iraP"
                     /locus_tag="QBF46_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDQ6674598.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         7901 a         8473 c         7828 g         7409 t
ORIGIN      
        1 ggcgaaacag aatgggcaat gcatacggcg tcctgaccag gtggttaaca gaaatctatt
       61 ttaccctgaa ctgacgatac aacaaaggcg caacgctttt gagataaagg atcgatgctt
      121 tcgctgcttt taccctctac cattaggcta attgcgacga gaaagggacg acctatgaat
      181 actaacgttt ttcgactgct cctgctggga agcctgttca gccttagcgc ctgtgtgcag
      241 cagagcgaag tgcgacagat gaaacacagc gtcagcacgc tgaaccaaga gatgacgcaa
      301 ctcaataaag aaacggtcaa aatcactcag caaaacaggc tgaatgcaaa atccagcagt
      361 ggggtttacc ttctgccagg agcgaaaaca ccggcaagac tggaaagcca gatcggtact
      421 ttacgtatgt cgctggtgaa tattacgcct gatgcagatg gcaccaccct gacgctacgt
      481 attcagggcg agtcgaatga tcccttgccc gcattcagcg gaactgttga atatgggcaa
      541 attcagggaa caatagacaa ctttcaggaa atcaatgtgc agaatcaatt gattaatgcc
      601 cctgccagcg tcctcgcccc cagcgatgtt gatattccgt tacagttaaa gggtatttct
      661 gtagaacagt tagacttcgt acgtatccac gatattcaac ctgttatgca gtaaacgtat
      721 ttcggggcga ttttgtgcgt cccgcaacat ctttccccgt cattttgtta ctccgcttac
      781 atcacccaga ttgatagtaa aagtttgcaa caagggtgaa agtcagtaca atccctgccc
      841 gaatgtgtgt aaacgtgaac gcaatcgatt acgtaaatga tagaactgtg aaacgaaaca
      901 tatttttgtg agcaatgatt tttataatag gctcctctgt atacgaaata tttagaaacg
      961 caatttgcgc ctttttcact cccgcaaggg attttcaaac agtggcatac atatgaaaaa
     1021 aacattactg gcagccggtg cggtactggc gctctcttcg tcttttactg tcaacgcagc
     1081 tgaaaacgac aaaccgcagt atctttccga ctggtggcac cagagcgtta acgttgtcgg
     1141 aagctatcac acccgtttcg gaccgcagat ccgcaacgat acctaccttg agtacgaagc
     1201 attcgctaaa aaagactggt tcgacttcta tggttatgcg gatgcgccgg tattcttcgg
     1261 cggtaactcc gatgcaaaag gtatctggaa ccacggttct ccgctgttta tggaaatcga
     1321 accacgtttc tccatcgaca agctgaccaa tactgacctt agcttcggtc cgttcaaaga
     1381 gtggtacttc gcgaacaact acatttacga catgggtcgt aataaagatg gtcgccagag
     1441 cacctggtac atgggtctgg gtaccgatat cgacactggc ctgccgatga gcctgtccat
     1501 gaacgtctat gcgaaatacc agtggcagaa ctatggcgca gcgaacgaaa acgagtggga
     1561 cggttaccgt ttcaaaatta aatactttgt gccgattacc gatctgtggg gcggtcagct
     1621 gagctacatc ggcttcacca acttcgactg gggttccgat ttaggggatg acagcggtaa
     1681 cgcaatcaac ggtattaaga cccgtaccaa taactctatc gcttccagcc atattctggc
     1741 tctgaactac gatcactggc actactctgt cgtagctcgt tactggcacg acggtggtca
     1801 gtggaacgac gatgcagaac tgaacttcgg caacggcaac ttcaacgttc gctctaccgg
     1861 ctggggtggt tacctggtag taggttacaa cttctgatta tgaaaatgcc gggatttatt
     1921 cccggcattt cagattgtta acaacgtgcg ctttgttcat gccggatgcg gcgtaaacgc
     1981 tttgtccgcc ccacaaaagc acgtaaattc aatatattgc tgaaataacg taggcctgat
     2041 aagcgtagcg catcaggcaa ttttacgttt taatcacttc ttcttgttca tcatcgcttt
     2101 caggtcagcg aatgggttgt acttcgcttc accgacatct ttctgcgcat cttcccctgc
     2161 gataacggtc gtaccatact gatccgcttc ggtatatttc gaatgctcat gatcgtggca
     2221 atagagacaa agcaattccc agttactgcc atcttccggg ttattggtat ggtcgtgatc
     2281 aatgtggtga acagtaagtt cacgcaggtt ggaataaaca aactcgcggg aacagcgacc
     2341 gcagacccac ggatatattt ttaatgcttt ttcgcgatag ccgctttcta accgcgcata
     2401 gttttttggg atgatagcca tgaagagtta cctggatgaa atgtagggtt acgcgcagtt
     2461 tcccaagatg cggggatttt cgcaatctcc atacatcaaa aaaaatcccg atcttctgac
     2521 cgggattttc aacatcaacg ggaacttgat tacggcagaa ttgacggctg atccgcccct
     2581 tctttttcca ctttctgctg caacatgtgt tcgcgcttca tacccagttt cagagccaac
     2641 gcagatgcca catagatgga agatgcagta ccgatggaaa caccgataag catggtcagc
     2701 gagaagcctt ccagtaccgg accaccgaag aggtacagca tcaggataac catcaaggta
     2761 gtaccggatg tgatcaaggt acggtgcagc gtctgggtca aggacacgtt aaagatttcg
     2821 taaggcgtac cgcgacggat cttgcggaag ttttcacgaa tacggtccga taccacgata
     2881 ctgtcgttaa gcgagtaacc gataaccgac atcaacgatg ccacaatggt caggtcaatc
     2941 tcgatatgga ataacgacaa aatacccagc gtaatgataa cgtcgtgcgc cagcgcaata
     3001 acaacccctg ccgccagtcg ccactcaaag cgaaaaccta cgtacacaag gatagacagc
     3061 agcgccgcca tcaacgccat cgcaccggtt tgcgcaaggt ctgcccccac gctcggaccg
     3121 acgaactcaa tacgcttcac tgctgcattc tgattggtgg attcgttaat caccttcaga
     3181 acctggctgc ccagcacctg accgccggtt tcgccttcag caggcggcat acggaccatg
     3241 atgtcgtggc tgctaccaaa gttttgcagc attggctctt caaaaccggc tttttgcaat
     3301 gcatcacgca ttacgtcaat ttcagccggt ttttcgagcg taatttcgat aaccgtacca
     3361 ccggtgaaat ccagccccca gttaaagccg cgcacgccca taataacgat agcggcgatt
     3421 aacagcagac cagagatgcc gaaagcccag tagtcccagc gcataaagtc atagacttta
     3481 cggccgtggt ttagttgttc aacagtatat tcctgtgcca catcgcactc ctcagattga
     3541 cagcttcttg acgcgcttgc cgccatatag caggtttacg atggcacgcg taccgacaat
     3601 cgcggtaaac atcgacgtcg ccacaccgat accggtagta atcgcgaacc ctttaattgc
     3661 cccggtaccc actgcgtaca ggatgatgac tttaatcagc gtggtgatgt tcgcatcgaa
     3721 gatagaactg aatgcgccac gataaccttc atcaattgcc tgttgaacag tacgtccgtt
     3781 gctcaactct tctttaatac gttcgttgat cagtacgttc gcatcgaccg ccaccgcaag
     3841 ggttaagacg atacccgcga tacctggcat actcagcgtt gcgcctggca acagcgacat
     3901 aatgccgacg attaagatca agttggcaat cagagcactg gtcgcaatca gaccaaactt
     3961 cttatagaag atgatcatga acagaataga aaccagcaga ccggcaaggc aagcctccag
     4021 cccctgttca atgttctgca tacccagggt tgggccaatg gtacgttctt caacaatctg
     4081 aatcggcgcg atcaacgcac cggcacgcag cagcagtgac agctgacggg cttcgttcgg
     4141 gttgttgatg ccggtgatac ggaagctgtt acccagacga gactggatgt tggcgatgtt
     4201 aatcacctct tcctgtttca ccagaaccgc acgaccattt gcatctttct taccgctgtc
     4261 tttgtactcc acaaacaggg ttgccatcgg tttgccgatg ttgtccttag tgaagttaga
     4321 catgatgtta ccaccagcgc tatcgagcga gatgttaacc tgcggttggt tgtattcgtc
     4381 ctggctggaa gtggagtcgg tgatatggtc accggtcaga attacgcgtt tgtacagcac
     4441 aactggctga ccttcgcggg tctgtttcac ttcagagtcg cccggtacgc gaccggatgc
     4501 cgcagcggcc tggtcaacgt tggtgtttac cagacggaat tccagcgttg ccgtcgcacc
     4561 cagaatctct ttcgcacgcg cagtgtcctg aatacctggc agttcaacaa cgatacggtc
     4621 agcaccctga cgctgaacca ccggctccgc cacgccaagt tggtttacac ggttacgcag
     4681 gatattgatg ttctgctgca ccgcatattc acgcgcttca ctcagacgag catcgctcat
     4741 taccgcacgc agctggttgc tgccctggct actaatcacc aggtccggat ggcgcttgct
     4801 cagatacgca atggcttcat cacgagcttt agcatcgcgg aaagtaatgc tcaggccgta
     4861 gttgttttct ttacgaacgg tggtatacgg gatgcccttt tcacgcagat cgctgcgcag
     4921 gctatcgata ttttgttcct ggagtttgcc aagcgcggtg tccatatcca cttccatcag
     4981 gaagtgaacg ccgccacgca ggtcaaggcc gagcttcatc ggctcagcgt gaatagctgc
     5041 cagccagcgc ggcgttgccg gggcaaggtt aagcgccacg acgtatttgt cacccataac
     5101 gcccattaat gcttcacgag cgcgcagctg ggtgtcagtg gagtcgaagc gcgcaagaat
     5161 agcgccctct tccagtgcca cagacttagc agttattttt tcttcttgta acgttttctg
     5221 gacctggatc agcgtttgct cactggcggc gactccgcgc gcaccagtga tctgaacagc
     5281 cggatcctca ccaaacaggt tgggaagcgc atacagcaga ccgatgacaa tcaccacgat
     5341 cagcatgacg tacttccaca aaggataacg gtttaacacg gcaattccct tagggaaaaa
     5401 ttttaattac agcgccttca tggtgccttt cggcaggacg gcagctacga agtcacgttt
     5461 aataactact tcagtggtgt cattcagcgc gatagcaatg tagccgtttt ccgctacttt
     5521 ggttacgcgg ccaaccaggc caccgttcgt cagaacttca tcacctttgg caatggagtc
     5581 catcagcttt ttgtgttctt tggtgcgctt ctgctgtgga cgcaggatca tgaaatagaa
     5641 aatcagaccg aataccacca gcatcaaaat caaagacatc gggctacctt gcgccggtgc
     5701 acccgttgcc gctaccgcat cagaaataaa aaagctcatt aaatttccct cattattaat
     5761 attaatcaac gttcaaaggt ggtacttctc gcccctgacg ctggtaaaaa tcagttacga
     5821 agctctctaa tttaccctct tcaatagcct tgcgtaaacc cgccatcaaa cgctggtagt
     5881 aacgaaggtt atgaatggtg ttgagtcgcg cgcctaatat ttcgttgcaa cggtcaagat
     5941 gatgcaagta agcgcgtgaa taattgcgac aggtgtagca atcacactca ggatcgagtg
     6001 ggccagtatc gctcttatac ttcgcattgc ggattttcac cacgccatcg gtcacgaaca
     6061 aatgaccatt tcgggcgttg cgggttggca ttacgcagtc aaacatatcg ataccacgac
     6121 gtacgccttc aaccaggtct tctggtttac caacgcccat caggtaacgc ggtttgtctg
     6181 ccggaatttg cgggcataca tgctccagaa tgcggtgcat atctgctttc ggctcaccca
     6241 cagccagacc gccgacagcg tagccatcaa aaccgatatc taccagacct ttaacagaaa
     6301 tatcacgtaa atcttcgtaa acgctgccct gaatgatacc aaacagcgca tttttgtttc
     6361 cgagactgtc aaaacgctca cggctacgct tcgcccaacg cagagacatc tccatggagc
     6421 gttttgcgta atcccagtca gcaggatacg gcgtacactc atcaaagatc atgacgatat
     6481 ccgaaccaag atcgtactga atctccattg atttttcagg atcgaggaaa atcggatcgc
     6541 cgttgatcgg gttacggaag tgcacgccct gttcggtgat tttacgaata tcgccaaggc
     6601 tgaagacctg gaagccgccg gagtcggtga ggatcggccc cttccactgc ataaaatcgt
     6661 gcagatcgcc gtgcagtttc atgatttcct ggcccgggcg cagccacagg tggaaggtgt
     6721 tgccgaggat aatttgcgcg ccagtggctt caacttcttc cggcgtcatc ccttttacgg
     6781 tgccgtaggt gccaacaggc ataaaacaag gcgtttccac tacgccacga tcaaagacca
     6841 ggcggccacg gcgtgcgcga ccgtcggtgg tgtccagttc aaatttcatt ttttctccac
     6901 tacgtcagaa aaacagtcca acgttttaaa ccagcgccgc ggaattactc cccgacgcgc
     6961 tcattaattg cctgcggatt gtacgtgata aacatcgcat caccgtaact aaaaaagcga
     7021 tatttctctt ctaccgctgc tttataggcg ttcatggtgt gttgataacc ggcaaaggcc
     7081 gaaaccagca taatcagcgt cgactctggc aagtggaagt tcgtcaccag cgcatcgacc
     7141 actttgtact ggaagcccgg atagataaag atttgggtat cgtcgaagaa cggttcaatg
     7201 agatcgtttt tcgctgcctg agccgcgctt tccagcgaac gtactgaagt ggtgccaacc
     7261 gcaatcaccc ggttaccgcg cgctttcgcc gccagtaccg cgtctaccac atcctgcggt
     7321 acttcagcgt attccgagtg catgatgtga tcttcaatgg tgtcgacgcg caccggctgg
     7381 aaggtgcccg caccaacgtg caacgtcaca aacgccatct ccacgccttt ggcgcgcaat
     7441 ttttccagca aaggctcgtc aaaatgcaga cctgcggtcg gggctgcaac cgcgcccggt
     7501 ttttcgctat aaacggtttg ataaagttcg cggtcagcgt cttcgtccgg acggtcgata
     7561 tacggcggca gcggcatatg gccgatgctg ttgagaatat ccagcaccga gcgttcatca
     7621 ttaaattcga cttcaaacag tgcgccgtgg cgcgcggtca ttgttgcgtt aatactttcg
     7681 tcatcgccca gcagcagttc tgcgccaggt tttggcgctt tcgaggcgcg aatatgcgca
     7741 agaatgcgtt tgtcgtcgag catccgttca accagcactt caatcttgcc gccgctggct
     7801 ttacgcccaa acaggcgcgc cgggatcacg cgggtattat taaaaaccag aagatcgccg
     7861 gggttgagct tatcaagtaa atcggtgaaa gtaccgtgcg tcagcgcgcc cgtcggcccg
     7921 tccagcgaca gtaaacgaca gctactgcgt tcaggcatgg gatagtgggc aatcagggat
     7981 tcgggcaatt caaaggagaa atcggtaacg cgcatgacac tgactcagac taaaataaga
     8041 ggcgggtagt ctagtgccgg ggcgctctcc ctgcaacctt tacccccttt tggatagaat
     8101 ataatcgatg aattttttag ctcacctgca tttagcccat ctcgcggaaa gctcgctttc
     8161 cggcaattta ctggctgatt tcgtacgcgg aaatcctgaa gaaagttttc cgcccgacgt
     8221 cgtggctggc attcatatgc atcgacgtat cgacgtattg actgacaatc tgccggaagt
     8281 ccgcgaagca cgggagtggt ttcgtagtga aacgcgccgc gttgcgccta ttacgctgga
     8341 tgtcatgtgg gatcactttc tttcccgcca ctggtcgcag ctgtcgccgg actttccgct
     8401 acaggaattt gtctgttatg cccgcgagca agtgatgacg attttgccgg actcaccgcc
     8461 acgttttatc aatctgaaca attacttgtg gtcagagcag tggctggtgc gctatcgcga
     8521 tatggatttc atccagaacg tgttaaacgg catggcaagc cgccgcccac gtctggatgc
     8581 cctgcgtgac tcctggtacg atttagacgc tcattatgac gccctcgaaa cccgcttctg
     8641 gcagttttat ccgcggatga tggcgcaggc gtcacgcaag gcgttataaa acgttagttc
     8701 atccataccg tagccgaaat ggcaggcaaa gccagaatcc cgtcagtcag ttgcccatgc
     8761 ccttctttgc attgccattg cacggcattg agaaacggtg acgcgggtag caccacttca
     8821 caggcctcgc cacggttgat tgccaccagt acacgttgct gattcagcac gcggacaaat
     8881 accaccacgt tatcttccgc atacagcacc tgacagccgc catgacgtag cgcctgactt
     8941 ttcttacgca gcgcaatcat tcgctggtac agcgcgaata acgccgtatc ctgcttttcc
     9001 acctgccagg ggaacggttt acggcaaaac ggatcgtttt tgccatccag tcctacttca
     9061 tcaccgtaat aaatgcacgg tacaccaggc caggtgaaca gccagaccac cgccagcggc
     9121 aggcgcgcaa tatcccgacc gagcagcgtt ttaaatcgcg cagtatcgtg gctgtcgagc
     9181 tgattaaaca tacgtaattg ttgttgatga gaaagccctg cgcggtaatt atccatccag
     9241 gccatacagg tttgggcatc aatttgctgc ggatcgtaag agatatcggt attggcaaga
     9301 aatccccaca acgggaatgt gaagccacga tagttcatgg cggcatcttc cacatcggcc
     9361 tgtaaccatt gccgtgcatc gccaaaatgt tcgccgacaa tatacgcttc cggctgggtt
     9421 tctttcgccg cttcggtgat cccggcaacg tgctgcatat tattgcgcgc cccacccgcc
     9481 tcccccagca tatgcaccac atccagccgc cagccgtcca tattccacgg cgctttcagc
     9541 cagtggcgga caatactgtc ttccccgcga taaatttcat tcaccagact ttccgactga
     9601 taatccagct tcggcaagct ggcatagcca agccagtcga gcgccgtgcc atcatcacta
     9661 aacgagtacc agtcgcgcca gggcgattcg gggttgtgac aagcaccacc cgtgccacga
     9721 ttatgcctgt caaaccaggc atgggaatcg ccactgtggt taaacacgcc gtccagcacc
     9781 agccgcattc ccagctgctg cgtattgtgt cgcaaacgca gcaacgcccc atcaccgcca
     9841 aactgcggat cgacatggcg ataatcctcg gtatcgtatt tatgtacgct gggagcttta
     9901 aacaccggat tgagatacag cgccgtcacg ccaagttttt tcagatacgg cagtttttcg
     9961 cttatcccgt ccagatcgcc gccatagaac gttgatccgc ccgcctgcgc cgtgaccggt
    10021 tcatcccagt cacgcaatat gatctcttgt ccggctgcgt gatggtaata gacatgatcc
    10081 tgttcagctt cacgaggaag actacgggcg aaacgatcag ggaagatctg ataaaaaatc
    10141 tgatccgcag cccattgtgg gccgatatcc ggtacatcaa cggcaaactg ctccagtcgt
    10201 gccggcggca ttcggctgaa gccctgcggt gtaaaccaac gctggcgatc gtgccaaagc
    10261 aatttgaaac tataacgccg ccggggttgt ccgctggaga gatcgatcgc cgcccgccat
    10321 gcggtgacgc ctggctgcgg ctgactgcgc tgcttatgca tcggtactga catttcttcg
    10381 ttatcgtgtt ctgtacgcag cataatgcgc tgcggtgggt cttcgcccgt cagccacagt
    10441 gtaatgagca gttgatcttt gctttgttta acaaatgggg gcaccggcag gtgccatgca
    10501 tttaacatca taattcccct gtgatgaaaa tctgatcacc ttgccatagc ctaatgcagg
    10561 atcactcatc atctgaagga tatttggggg aggaggacgg gggcggataa ctttcggaat
    10621 agccggatca ggatttcctc ctgaaccggc ttacagaacg caaggattac tggttttcag
    10681 ccagctgacg atcgtggcgg cgtttaaaca tccagccaat caacagcaca acaatccacg
    10741 cgaaaccgac atacagcgag atacgcgtat ccgggtgata accaatcaac ccgataataa
    10801 agagcaggaa aatcagcccg ccgatggtcg ttgctacccc acccggcact ttaaatttca
    10861 gcgccttaac gtcttctggc ggcaaacggc gacggaaggc aatttgcgac agcaggatca
    10921 taatccacac ccacaccgtg gcgaaggttg ccagcgaagc gatcaccagg aagacgtttt
    10981 ccggcatgat gtagttcaga tacaccgcaa acagcagcgc ggtagtcatc accagcaccg
    11041 taacccacgg aataccgcga cgcgacgttt tgctgaaaat tttcggtgcg ctgccctgct
    11101 ctgccatacc gtggagcata cggcctacgc caaatacatc actgttaatt gccgacagcg
    11161 aagcagtcag cacaacaaag ttaagaatgc tggcggcaaa ggtaatgccc atatgctgga
    11221 acgtcagcac gaacgggcta ccggcagtgc caacctgatt ccacgggtag atagacataa
    11281 tgacgaacag cgtaccgacg tagaacacca gaatacgcat cggcacggag ttaatcgcac
    11341 gcggtatcga tttctcagga tctttcgctt caccggcggt aatcccgata atttcgatcc
    11401 caccgtaagc aaacatcacc atttgcaacg acattaccat gccaagccag ccgttactga
    11461 agaagccgcc gttgctccac aggttatgaa taccggtcgg ttgcccgccg ttgccaatcc
    11521 cccagatgat gatgccgaaa ccggcgacaa tcatgatgat gatggtggcg actttaaaga
    11581 acgagaacca gaattccagc tcaccgaata ccttcacgct catcaggttt acggcgcaaa
    11641 tgatcagcac cacgctcagt acccaaatcc agtgcggcac cgtcgggaac cagacaccca
    11701 tatagatacc aaaagcggtc acatcggcga tggcgacaat aaggatttca aagcagtagg
    11761 tccagccggt aatgtaacct gccagcgggc cgaggttttc ctgcgcataa cgcgagaaag
    11821 agctggcggc cgggttatgt accgacattt cccccagcgc acgcataatg atatacgccg
    11881 cgataccacc gataatatag gccaacaaca cgctcggacc ggccattttg atggcgtctg
    11941 ccgaaccgta aaacagcccg gtgccaattg ctgaacccag tgccataaag cgtatgtggc
    12001 gggtacttag cccacgcttt agcttgttct tactttccat caattcctaa cccatcaaca
    12061 caataaaaaa ccacggggca tcgcagcccc gtggttaaaa caaatgttca gtgatttagt
    12121 gagcgctgga ggtcacctga cgacctgccg cgcgatccca gataatggcc agaaccacca
    12181 tcaccactgt tggcattaac cacgccagac cttgttcggc cagcggtaaa cgctgcgccc
    12241 aggacggtaa gatatcgctg aatgcagatg ctttgatccc gtcgagaata ccaaaaagca
    12301 ggctgataaa catcggcgga gcaatcacgc gggacgaatt atgccaccat gagcgtgtaa
    12361 aacttaatac aaccagtgcg atacacggcg gataaatagc ggtcagcacc ggtacggaga
    12421 tctggatcag ctggcttaag ccgaggttag aaaccaccat cgagaagccg ccgaggataa
    12481 acaccagcgt acgataagag agcggtacgt actgggcaaa gaattctgca caagcacagg
    12541 tcaggccaac tgccgttacc aggcaggcga tgaagattaa cgccgccagc aggaagctac
    12601 cgccgccgcc aaaggtgtgc tgaacgtaag catgcagaat agcagcgccg tttgcagact
    12661 gatcgaccag cgacgcgctg tctgacccca gacggaacag cgccaggtac agcagagtca
    12721 gaccaacacc cgccatcagg ccagcccaga cggtataacg ggtcagcaga cgcgcttcgg
    12781 taacgccacg agaacgcgcc gcgttaacaa taacgatacc aaacaccatt gcgcccagcg
    12841 tatccatggt cagatagccg ttaacgaagc cgttagaaaa cgcagcgttt tgataagcct
    12901 cagtcgccgt gctgatagaa cccgccggcc agacaatagc ggcaacagac aggatgacca
    12961 gcgcgataat tttcagcggc gcaaggaagt tgcccacggt atccagcagc ttgcccggat
    13021 agagcgaaac cagaataacg atagcgaaat agaccaggct gtagataaac agcggcagcg
    13081 cggaatcacc cgtcagcggc gcaatcccca cttcaaagga aacggtagct gtacgcggcg
    13141 tagcgaaaag cggccccacc gccaggtaac aaaccgttgc cagcagtacg ccagcgactt
    13201 taccgattgg cgtgctgagg ctgtcaacac cgccgccaac ttttgccagc gccactaccg
    13261 ttaataccgg caggccaacg gcagtaatga ggaagccgaa tgccgcagtc cagacgtgtt
    13321 cgcctgcctg taagccgacc attggaggga aaataatgtt acctgcgccg acgaacaacg
    13381 caaatgtcat aaagcccaga gcgatgatat cgcgcgatct taattgatgg gtcataaatc
    13441 atactgcctg tggatgtggt gttgtaacgt taaatttttg tgccaatccc tggggatgat
    13501 acagcggtat atttaaacaa ttttagcggg aaatactccc aaccaggcgc ggcgaagaat
    13561 agtttttcga tttaccgcgt taatgcagtc agtatgacag cacctgaagc gcaatttaaa
    13621 cgcttataac gttttaaagc aaggagggat ctaaaaacca gaggtatata gcgataagaa
    13681 atcaccaacc agtataaaac actatttccg tgaaaaagac atggctaatc atgcgaacaa
    13741 aaaatcacca accgttgtaa aatctaacgc ccgtattttg actgcaaaga aaataagcca
    13801 gcatcgactg gcttatggca ataaaagatg acaaaggcgg attaatcgct gtttttggca
    13861 attaaacgtt ccgggataac aaaactgaaa cgtgttcctt ttcctactgt actctcaata
    13921 ttcaggcgac tttcgtgatg attcacagca tgtttcacga tcgctaaccc taatccgcta
    13981 ccgccggttt gccgggaacg cgctttatca acgcgataaa aacgctcggt caggcgcgga
    14041 atatgctccg gtgcaatgcc cggtccgtta tcttcaacgc taaattcggc accgtgcggc
    14101 actcgctgcc agcgtacggt gatatgcgtg ccttccggcg tatgattcac ggcgttatag
    14161 accaggttcg aaatcgcact gcgtagctga tcttcgttgc cagacacctt gaggccgtta
    14221 tctatctcaa aggtaaatgt ctgttttttc tgactcagag tctgagcctc gcgctcaaca
    14281 acgcgcagca tcatcggcac atcaaccttt tcattgagca aatgcgtcgg tgcggcttct
    14341 attttcgaca gcgtcagcaa ttgcttcacc agtccttcca tccgctgggt ctgctcgcgc
    14401 atggtgtgca acgctttttc gcgtaccgcg ccttccagcg gctgctcatt catcatctcc
    14461 aggtaaccct gtaacacggt caatggcgta cgtaactcat ggctcacgtt ggcaaaaaag
    14521 ttacgccgcg ccccttccag ttgatgcatt tgcgtgacat cacgcgccac catcagcaac
    14581 tgtttgtggg tataaggcat gacgcgaatt tccagatgcc gcccggtgtt gagcaccaga
    14641 ttgagcgggc gagaaaaatc acgcgttttc agatattgcg taaactccgg gtaacgcagc
    14701 aggttaagga tgttctgccc gttatcttcc ggccagcgca aaccaagaat ctgttgcgcc
    14761 agaccgttac accagaaaat accgccctct tccgtggtca gcaccactgc gtcgggcagc
    14821 gactccgcgc cgctacgaaa gcgtttaatc agattgccca gttcacggcg gcgtttttta
    14881 tttcgcagct gcatctggtg taagccgtat agtagcggtt cccagctacc acgccccggt
    14941 ggcggggtca tactacgatc cacccacagc caccatgaaa ggcgcaataa attccagaaa
    15001 tgccagataa gcagtcccgt taccgatgcc agcaaaaacc agggcaggta accaaaaaat
    15061 gcacccagga tgaaagccgg gaggcagcaa agtaacagct ccagcaccag ccttttccac
    15121 gacagccgtt ccagcacgta agatactcca gttaagaaat cataagccct gctctgcgtc
    15181 cgatgagcaa ggcgttaaaa gcgggttgaa aaacgatatc ctgtaccgcg cacggtctgc
    15241 accatgcggt catgcccgcc gggctccagt gctttacgca ggcgacgaat gtggacatcg
    15301 accgtgcggt cttccacata cacgttagtt ccccagacgt ggtttaacag ctgctcgcgg
    15361 ctgtacacgc gctcaggatg cgtcataaaa aagtgcagca gtttaaattc tgtcggcccc
    15421 atctccagcg gctcttcgcc cgccatcacc cggtgagatg tcgggtcgag acttaatccc
    15481 tgcatctcaa tcacctcttc caccgccatt ggcgaaatac ggcgcattac cgctttgatt
    15541 cgcgccacca gctccttcgg cgaaaacggc ttggtgatat agtcatccgc gccggtttca
    15601 aggccgcgca cgcgatcttc ttcttcccct ctggcggtca acatcaccac tggaatatcc
    15661 cgggtcatcg actcgcgctt gaggtgttta atgaactgga taccggagcc gccaggtaac
    15721 atccagtcaa ggagaattaa atccggccag ggttcattca gttgattcac agcactgtca
    15781 taatcttccg cttcgaccgg ctgaaagcca ttttgttcga gcacgaagca gaccatttcg
    15841 cgaattggag cttcatcttc tacgaccaga atacgtctcg ccatgatttg ccctgttgta
    15901 ataaataggt tgcgatcatt aatgcgacgt cattatgcgt cagatttatg acagatttat
    15961 gaaaagctcg tcgcacatat cttcaggtta ttgatttccg tggcgcagaa aaaagcaaat
    16021 ggcacatctg tttgggtata atcgcgccca tgctttttcg ccagggaatc gttatgcgca
    16081 tccttcacac ctcagactgg catctcggcc agaacttcta cagtaaaagc cgcgaagctg
    16141 aacatcaggc ttttcttgac tggctgctgg aaacagcaca aacccatcag gtggatgcga
    16201 ttattgttgc tggtgatgtt ttcgataccg gctcgccgcc cagttacgcc cgcacgttat
    16261 acaaccgttt tgttgtcaat ttacagcaaa ctggctgtca tctggtggta ctggcaggaa
    16321 accatgactc ggtcgccacg ctgaatgaat cgcgcgatat catggcgttc ctcaatacga
    16381 ccgtggtcgc cagcgccgga catgcgccgc aaatcttgcc tcgtcgcgac gggacgccag
    16441 gcgcagtgct gtgccccatt ccgtttttac gtccgcgtga cattattacc agccaggcgg
    16501 ggcttaacgg tattgaaaaa cagcagcatt tactggcagc gattaccgat tattaccaac
    16561 aacactatgc cgatgcctgc aaactgcgcg gcgatcagcc tctgcccatc atcgccacgg
    16621 gacatttaac gaccgtgggg gccagtaaaa gtgacgccgt gcgtgacatt tatattggca
    16681 cgctggacgc gtttccggca caaaactttc caccagccga ctacatcgcg ctcgggcata
    16741 ttcaccgcgc acagattatt ggcggcatgg aacatgttcg ctattgcggc tcccccattc
    16801 cactgagttt tgatgaatgc ggtaagagta aatatgtcca tctggtgaca ttttcaaacg
    16861 gcaaattaga gagcgtggaa aacctgaacg taccggtaac gcaacccatg gcagtgctga
    16921 aaggcgatct ggcgtcgatt accgcacagc tggaacagtg gcgcgatgta tcgcaggagc
    16981 cacctgtctg gctggatatc gaaatcacta ctgatgagta tctgcatgat attcagcgca
    17041 aaatccaggc attaaccgaa tcattgcctg tcgaagtatt gctggtacgt cggagtcgtg
    17101 aacagcgcga gcgtgtgtta gccagccaac agcgtgaaac cctcagcgaa ctcagcgtcg
    17161 aagaggtgtt caatcgccgt ctggcactgg aagaactgga tgaatcgcag cagcaacgtc
    17221 tgcagcatct tttcaccacg acgttgcata ccctcgccgg agaacacgaa gcatgaaaat
    17281 tctcagcctg cgcctgaaaa acctgaactc attaaaaggc gaatggaaga ttgatttcac
    17341 ccgcgagccg ttcgccagca acgggctgtt tgctattacc ggcccaacag gtgcggggaa
    17401 aaccaccctg ctggacgcca tttgtctggc gctgtatcac gaaactccgc gtctctctaa
    17461 cgtttcacaa tcgcaaaatg atctcatgac ccgcgatacc gccgaatgtc tggcggaggt
    17521 ggagtttgaa gtgaaaggtg aagcgtaccg tgcattctgg agccagaatc gggcgcgtaa
    17581 ccaacccgac ggtaatttgc aggtgccacg cgtagagctg gcgcgctgcg ccgacggcaa
    17641 aattctcgcc gacaaagtga aagataagct ggaactgaca gcgacgttaa ccgggctgga
    17701 ttacgggcgc ttcacccgtt cgatgctgct ttcgcagggg caatttgctg ccttcctgaa
    17761 tgccaaaccc aaagaacgcg cggaattgct cgaggagtta accggcactg aaatctacgg
    17821 gcaaatctcg gcgatggttt ttgagcagca caaatcggcc cgcacagagc tggagaagct
    17881 gcaagcgcag gccagcggcg tcacgttgct cacgccggaa caagtgcaat cgctgacagc
    17941 gagtttgcag gtacttactg acgaagaaaa acagttaatt accgcgcagc agcaagaaca
    18001 acaatcgcta aactggttaa cgcgtcagga cgaattgcag caagaagcca gccgccgtca
    18061 gcaggccttg caacaggcgt tagccgaaga agaaaaagcg caacctcaac tggcggcgct
    18121 tagtctggca caaccggcac gaaatcttcg tccacactgg gaacgcatcg cagaacacag
    18181 cgcggcgctg gcgcatattc gccagcagat tgaagaagta aatactcgct tacagagcac
    18241 aatggcgctt cgcgcgagca ttcgccacca cgcggcgaag cagtcagcag aattacagca
    18301 gcagcaacaa agcctgaata cctggttaca ggaacacgac cgcttccgtc agtggaacaa
    18361 cgaactggcg ggttggcgtg cgcagttctc ccaacaaacc agcgatcgcg agcatctgcg
    18421 gcaatggcag caacagttaa cccatgctga gcaaaaactt aatgcgcttg cggcgatcac
    18481 gttgacgtta accgccgatg aagttgctac cgccctggcg caacatgctg agcaacgccc
    18541 actgcgtcag cgcctggtcg cgctgcatgg gcagattgtt ccccaacaaa aacgtctggc
    18601 gcagttaatg gtcactatcc agaatgtcac tctggaacag acgcaacgta atgtcgcact
    18661 taacgaaatg cgccagcgtt ataaagaaaa gacgcagcaa cttgccgatg tgaaaaccat
    18721 ttgcgagcag gaagcgcgca tcaaaacgct ggaagcccag cgtgcacagt tacaggcggg
    18781 tcagccttgt ccactttgtg gttccaccag ccatccggcg gtcgaggcgt atcaggcgct
    18841 ggagcctggc gttaatcagt ctcgattact ggcgctggaa aacgaagtta aaaagctcgg
    18901 cgaagaaggt gcggcgctgc gcgggcaact ggatgcatta acgaagcagc ttcagcgcga
    18961 tgaaaacgaa gcgcaaagcc tccgacaaga tgagcaagca cttactcaac aatggcaagc
    19021 cgtcacggcc agcctcaata tcaccttgca gccacaggac gatattcaac cgtggctgga
    19081 tgcacaagat aagcacgaac gccagctgcg gttactcagc caacgacatg aattacaagg
    19141 gcagattgcc gcgcataatc agcaaattat ccagtatcaa cagcaaattg aacaacgcca
    19201 gcagcagctc ttaacggcat tggcgggtta tgcactgaca ttgccacagg aagatgaaga
    19261 agagagctgg ttggcgacac gtcagcaaga agcgcagagc tggcagcaac gccagaacga
    19321 attaaccgcg ctgcaaaacc gtattcagca gctgacgccg attctggaaa cgttgccgca
    19381 aagtgatgat ctcccgcaca gcgaagaaac tgtggcgctg gataactggc ggcaggttca
    19441 tgaacaatgt ctcgcattac acagccagca gcagacatta cagcaacagg atgttctggc
    19501 ggcgcaaagt ctgcaaaaag cccaggcgca gtttgacacc gcgctacagg ccagcgtctt
    19561 tgacgatcag caggcgttcc ttgcggcgct aatggatgaa caaacactaa cgcagctgga
    19621 acagctcaag cagaatctgg aaaaccagcg ccgtcaggcg caaacgctgg tcactcagac
    19681 agcagaaacg ctggcacagc atcaacaaca ccgacctgac gggttggctc tcactgtgac
    19741 ggtggagcag attcagcaag agttagcgca aactcaccaa aagttgcgtg aaaacaccac
    19801 gagtcaaggc gagattcgcc agcagctgaa gcaggatgca gataaccgtc agcaacaaca
    19861 aaccttaatg cagcaaattg ctcaaatgac gcagcaggtt gaggactggg gatatctgaa
    19921 ttcgctaata ggttccaaag agggcgataa attccgcaag tttgcccagg ggctgacgct
    19981 ggataattta gtccatctcg ctaatcagca acttacccgg ctgcacgggc gctatctgtt
    20041 acagcgcaaa gccagcgagg cgctggaagt cgaggttgtt gatacctggc aggcagatgc
    20101 ggtacgcgat acccgtaccc tttccggcgg cgaaagtttc ctcgttagtc tggcgctggc
    20161 gctggcgctt tcggatctgg tcagccataa aacacgtatt gactcgctgt tccttgatga
    20221 aggttttggc acgctggata gcgaaacgct ggataccgcc cttgatgcgc tggatgccct
    20281 gaacgccagt ggcaaaacca tcggtgtgat tagccacgtt gaagcgatga aagagcgtat
    20341 tcctgtgcag atcaaagtga agaagatcaa cggcctgggc tacagcaaac tggaaagtgc
    20401 gtttgcagtg aaataactat tcagcaggat aatgaataca gaggggcgaa ttatctcttg
    20461 gccttgctgg tcgttattct gcaagctatc actttattgg ctacggtgat tggtagccgt
    20521 tctggtagtt gtgatggtgg tatgaaaaaa gtcattttat ctttggctct gggcacgttt
    20581 ggtttgggga tggccgaatt tggcattatg ggcgtgctca cggagctggc gcataacgta
    20641 ggaatttcga ttcctgctgc cgggcatatg atctcgtatt atgcgctggg agtggtggtc
    20701 ggtgcgccaa tcatcgcact cttttccagc cgctactcac tcaaacatat cttgttgttt
    20761 ctggtggcgt tgtgcgtcat tggcaacgcc atgttcacgc tctcttcgtc ttacctgatg
    20821 cttgccattg gtcgcctggt ttccggcttt ccgcatggcg cattttttgg cgtcggcgcg
    20881 atcgtgttat caaaaattat caaacccgga aaagtcaccg ccgccgtggc ggggatggtt
    20941 tccgggatga cagtcgccaa tttgctgggc attccgctgg gaacgtattt aagtcaggaa
    21001 tttagctggc gttacacctt tttattgatc gctgttttta atattgcggt gatggcatcg
    21061 gtctattttt gggtgccgga tattcgcgac gaggcgaaag gaaagctgcg cgaacaattt
    21121 cactttttac gcagcccggc cccgtggtta attttcgccg ccacgatgtt tggcaacgca
    21181 ggtgtgtttg cctggttcag ctacgtaaag ccatacatga tgtttatttc cggtttttcg
    21241 gaaacggcga tgacctttat tatgatgtta gttgggctag ggatggtgtt gggaaatatg
    21301 ctaagtggca ggatttcagg acgttattca ccactgcgca ttgcagcagt gactgacttt
    21361 atcattgtac tggcactgct gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt
    21421 atttttgctt ttatttgttg cgcgggatta tttgcccttt cagcacctct gcaaatatta
    21481 ttactgcaaa atgccaaagg cggagagtta ttaggtgccg caggtgggca aatagcgttt
    21541 aacctcggta gcgccgtcgg agcatattgc ggaggtatga tgctgacgct ggggctggca
    21601 tataattacg tggcgctgcc tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg
    21661 ctgtatggtc gctataagcg ccagcaagcg gcggatagtc cggtgctggc gaaaccactg
    21721 gggtagatta ctgtcacggt gcgcgttgct tattgccgga tgcggcgtga acgccttatc
    21781 cgccctacgc ggttctggca cattttgtag gcctgataag acgcggcaag cgtcgcatca
    21841 ggcatgatgc gccaattgcc tacgtttttt actcttgtgg ccataaccac gcagcgccgc
    21901 gtacgccgct ggaatcaccg tgcttcgcct tacgcaccgg cgtttcacat tcgccgccga
    21961 agacaaattg tttaatcaac tgcccaaccg tttgatataa acggtctaca ttgctcatcc
    22021 cgccccccag gacaatcaca tccggatcga gaatattcac gacatgtgcc agcgattttg
    22081 ccagccgcag ctcgtagcga cgcaatgcca gttccgctac cggatcgctt tcttcaacca
    22141 ggcggataat ttcactgcct ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga
    22201 atcccgtgcc cgaaataaag gtttcaatac aaccttgttt accgcaataa caagggactt
    22261 cctcgcgata acgcagttcg tcttcgtcca tccacggtag cggattgtgt ccccactcac
    22321 ctgccgtgcc attgccgccg atatgcgccc gcccattgaa tgccacgccc gcgccgcatc
    22381 ccgtgccgat aatcacggca aataccgtct gtgctcccgc tgccgcgcca tctactgctt
    22441 ctgaaaccgc cagacagttg gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc
    22501 ttaagtcttt atcgaatggc tgaccgttga gccaggttga attggcattc ttcaccacac
    22561 cggtgtaggg cgaaatcgaa cctggaatgc ccatacctac cgttccgtgc tgccctgtcg
    22621 cctgctccgc catatcaacc aacgtggcga tcgtttcaat agtctgccgg taatcatcac
    22681 gcggcgtggg cagacgatgg cggtacaact gctcccctgc atcgcccagt gcaataactt
    22741 cagttttggt gccgcctaaa tcgataccta tacgcactgt actctcctta tttttttcaa
    22801 tatcaatagc gtagagacgg acaaccggat tggcaatgca aggccgccga caattcgtta
    22861 tcatgcccgc taaatttaac gacaaggccg tggaaattat catgctgtgg ttcaaaaatt
    22921 taatggttta ccgtcttagc cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc
    22981 tagcctcgat ggcatttacc ccatgcggca gccaggacat ggcgaagatg ggctgggttc
    23041 ctccgatggg atcgcacagc gatgcgttaa cgcacgttgc caatggtcaa attgttatct
    23101 gcgcgcgcaa agaagaaaaa attctcccgt ctccggtgat taaacaggcg ctggaagcga
    23161 aaatcgccaa actggaagcg gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc
    23221 tgaaagacga agtgctgcac tctctgctgc cgcgtgcttt cagccgtttt agccagacaa
    23281 tgatgtggat cgacacggtt aacggtttga ttatggtgga ctgcgccagt gccaaaaaag
    23341 cggaagatac gctggcatta ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga
    23401 gcatggaaaa cccgattgaa ctgacgctga ccgaatgggt tcgctccggt agtgcggcac
    23461 agggcttcca gctgcttgat gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga
    23521 tccgcgcgaa gaaacaagat ctgaccagcg aagagatcac caatcacatt gaagccggaa
    23581 aagtggtgac taaactggcg ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg
    23641 atggttcgct caagcgtctg aagttctgcg acgagctgcg cgatcaaaac gaagatatcg
    23701 accgtgaaga tttcgcccag cgttttgatg ccgatttcat cctgatgact ggtgaactgg
    23761 cagcgttaat tcaaaacctg attgaaggat taggtggcga agcacaacgt taattgctga
    23821 ttttccttta atgccggatg agactcctgc cgcgtcttat ccggcgtacg aagccacacc
    23881 aggcatataa ttattcgcta cggcgagcaa taatttttag cgcagcaata ttatgcgttt
    23941 tacgctgtaa cttgctccat ggacgttgtg tcattgtttt tcctcaagcc ggtgacgcgc
    24001 ttcactgcga attttccgcg ttgccccggt tttcactaaa tcacgtaaca gcgaaagatc
    24061 gggttgttcg acaaataaga gtaatgatgg atcctctttt aaccacgccg ttttcacatc
    24121 ggcagccagc tgtggattat taatagccag cgaccgatcc tgaggctcag tcaatgtcgt
    24181 taacaacaac gccggggtat gtgcgttgcg caatgccgtt tggcgtaaat gatcattgcg
    24241 actttgcagc aatgcacgcc accattcatc atctgattta tcttttatcg tagccagttc
    24301 cgcaacaata tctggagatc ttcactgata tttatatcaa ccttgataaa ttatgaatat
    24361 atcggaaatt gaaaaaatcg cagcaaagga aatattatta tgggttaaag aaaggatatt
    24421 tcagaaaaat gccctcccgt tcaaggggaa ggcgaggaat tacagatagc ggcacagata
    24481 agaggtgggt tcggcaactt gcagatgaaa ctcactgtga ccgggaacat taaacaccga
    24541 accggcttca tacacctgcc agtcggtcgc gtcaggcagt aacacattca gcgcgccact
    24601 gattaccgtc atctcttccg gctcagcggt gctgaaggtg tattcgcctt caaccataac
    24661 acccacgctg gcgcgaccag tgctgctgct ggaaaagccg attgatttca ctttgccgga
    24721 aaagtactca ttactttgaa gcataaactg gccctgtttg gtagttgaac atctacttca
    24781 ctataggggc cagaggcgct ggctgtcacg caaaattaca ccagtaattc cgcagccagc
    24841 cgtgcaatca atacgttaga cagcaacaca ggaacatcga gctgtttttg cagtaaatcg
    24901 cgatgacgtt ggtgaaatcc taaacaatcc agcatgatga catcagcccc ttttgccagt
    24961 aattctttcc cggcatcaat gattttttgt tctgaatcat gaatggggtt acccaatgaa
    25021 aataccggcg atttctgcaa aatttgccat ttttgcgcct gaacgggcag catctcctca
    25081 accggaacga taacccccac ctgatgatct tcaacaatag aggaaaccag tggaggcaat
    25141 attcgcgacg gctcaagaaa gatcgtatta cgcgcagtca tactactaat gtttgctgta
    25201 ctcattaata aaatgacgtc ataaccctga ttatcgagca cttcaaccac accttgcagg
    25261 tcacgctcca ctttgcgacg cgaaacatgg gccagctggt tgtcatttaa taatgtgaga
    25321 atggtgtctt cgcctgcttc tggcgcgtac tccgccatca cttcttcacg acttaacttc
    25381 cccagcaggc tatgatggga aatattatct tcgtcaatgt attccgtcag gagcggcaaa
    25441 acttcctgca taggtacaat gccgatggtc aggatcgcca acgacgcact cattgttcat
    25501 ccaccttact tttctttcgt cgttaattac cgggcaagtg tgaagcacca ctgctgacac
    25561 ttacttgtgc tacaaatgac aaaaagcgta gcagcagacg tgcacggcat aatgtaaaga
    25621 ttccaaatga ttccagtaat ggattgttat tgtttaatat tctaattatt agaaaaacat
    25681 gaattatgaa aaatgtgacg cagatccctt gtagtggcac tcagaatccc ttccgagcag
    25741 tctgatgcgg tcactctttg tcagggtcct catagcgttt tttcgcatcc atcgcttcct
    25801 gagcggtcgg atgttcactg atcaacgagt ctggtttagg atgatcggct ctgagttggt
    25861 accaggttac cgtctgtcct ggttttcctt tttcaatcgt cactatatat gcttcacgag
    25921 gataaggcgg tttcgttggc atcgttcttc cttatttcac gggatgaacg ttaagtatag
    25981 gcgctcgaaa atcaacaatt gatcgtctgt gccagggcgc tacgaatttc agaaatcacc
    26041 tggctgggtt cgtttgttgc gtcgatgata atatgcgcaa cttcgcgata tagcgcatcg
    26101 cgttcttcca gcacttcctg aacttcttcg ctcagcggtt ttcccgttaa ggttggccgt
    26161 aaatcttctt ccggtgcagc ttgcagtcgg ttaaccagga ctgatactgg cgcacacaaa
    26221 taaaccacga tcccgttatt ttgcatgaag tgacgattaa attccgtcag aataatgccg
    26281 ccgcctgtag cgataacggt ggatggcgca gttaccgctt ccagcgccgc cgtttctctg
    26341 gcgcgaaatc ccgcccactc ttccctttcg acgatctccg cgaccgtcat attgagctgt
    26401 gattgcaacc actgatcggt atcgacaaac cgacggttaa gcgaatcggc aagggccatt
    26461 ccgaccgttg ttttaccaca gccccgaggc ccgatcagaa aaagaggttg tgtcatcgtg
    26521 ggttttcccc aataggtcgc aatgcggcga aagccggtgt catgagaata gcgatcatac
    26581 catcaaacta gtacaatttc gattgtaaag gaaaaattcc acttaaagtg aaaatctcaa
    26641 tacacccctt actataccaa taaatattca agaatgaagt gtaaataata aattacattt
    26701 ggccgcgact acgttgcact tccagccacc atttctccag ctccgcagca aaggcctggc
    26761 ggtcacgttg tgaaagacta tctggtccgc cggtctggat cccactggca cgtaaggtat
    26821 ccataaaatc gcgcatcgtc aggcgctcac gaatggtcgc tggcgtgtaa cgttcgccgc
    26881 gcggattaat cgccgcagcg cctttctcga tggcttcagc agccaaaggt atatctgcgg
    26941 tgatcaccaa atcgcccgct tcacactgcc ggacaatttc gttatcggca acgtcgaaac
    27001 ctgccgcgac gcgcagcgta cgaataaatc gcgatggcgg cacgcgtaaa ctctggtttg
    27061 ctaccagtac cagcggcatc tgcatacgtt ccgccgcgcg atacaaaatc tctttaatta
    27121 cattgggaca cgcgtcggca tccacccaaa ttgtcataaa gtcatccttt gttgggtaat
    27181 cctctattgt gtcgcgcttt tgccttccgg catagttctg tttatgcttc tgccagcgat
    27241 tatcaaaaca atgaatttca cggcaggagt gaggcaatgg aaaagaaaat cggttttatt
    27301 ggctgcggca atatgggaaa agccattctc ggcggtctga ttgccagcgg tcaggtgctt
    27361 ccagggcaaa tctgggtata taccccctcc ccggataaag tcgccgccct gcatgaccag
    27421 ttcggcatca acgccgcaga atcggcgcaa gaagtggcgc aaatcgccga catcattttt
    27481 gctgccgtta aacctggcat catgattaaa gtgcttagcg aaatcacctc cagcctgaat
    27541 aaagactctc tggtcgtttc tattgctgca ggtgtcacgc tcgaccagct tgcccgcgcg
    27601 ctgggccatg accggaaaat tatccgcgcc atgccgaaca ctcccgcgct ggttaatgcc
    27661 gggatgacct ccgtaacgcc aaacgcgctg gtaacccccg aagataccgc tgatgtgctg
    27721 aatattttcc gctgctttgg cgaagcggaa gtaattgctg agccgatgat ccacccggtg
    27781 gtcggtgtga gcggttcttc gccagcctac gtatttatgt ttatcgaagc gatggccgac
    27841 gccgccgtgc tgggcgggat gccacgcgcc caggcgtata aatttgccgc tcaggcggta
    27901 atgggttccg caaaaatggt gctggaaacg ggagaacatc cgggggcact gaaagatatg
    27961 gtctgctcac cgggaggcac caccattgaa gcggtacgcg tactggaaga gaaaggcttc
    28021 cgtgccgcag tgatcgaagc gatgacgaag tgtatggaaa aatcagaaaa actcagcaaa
    28081 tcctgatgat ttttgccgga cgtcaggccg ccacttcggt gcggttacgt ccggctttct
    28141 ttgctttgta gagcgccaaa tctgccgatt tcaaccactc gcgatagtga ctcatttgtg
    28201 gattcagcgg cgcaaccccc acactaatcc gtaatgttac ctgtggcgta ttcggcaaac
    28261 gtaacgtgtt tagcccttca tgcacccgta acatggcggt aatggcgctc tcagctggtg
    28321 taccggacat gattaccgca aactcatcgc cgccaaaccg accaatcaca tcgctaccgc
    28381 gcagggttat ttgtaactgt cgggtaagcg ccacaatcgc ttcatcgccc acatcgtggc
    28441 cccaggtatc gttgatgctc ttgaaatggt cgatatcgat aatcagtaat gtcgcatcgc
    28501 gattatgccg ccgacagtta tcaaattcat tgcgtaacat agtttcccaa tgacgtcggt
    28561 tatacacgcc cgtcataccg tcgcgggtac tcatgacctg caacctgcgt ttatgttccg
    28621 ccagtttagt cgccgtctgg tagctgaccc agccaaacag cagaggataa acgacaataa
    28681 tgggaaggga gagccaccat tccagcggcg cactattgaa cgacacggtg atgcccgtca
    28741 gttcgagggt gacaaggcag gaaaccacca tcaacaccag acccgcgaca aacagacggg
    28801 ggccgcctgc ccccatcaaa ttcagacaca taatcatcaa catcgcggtg gaaggcagca
    28861 cgtttacgcc cattacgcct acccacattc ccgctaatac tgcatcggtt tttaagttgt
    28921 aaatttcccg gctaagcgga tcgacggccc tgctcgctat ctgccaggct aaatgcggcc
    28981 agacgaacgc ccagccgacc aacaccagcc accaccagcc cggcggcggg tgcgaaacca
    29041 gcgttgaagc aatcggtaag aacatgccag ccaggccaac cgcacggggt agtctgacgc
    29101 gacgggcgaa gcgcagcccg gaccgctgat gttcgttttg aggagttaaa ggaggttcct
    29161 ccccatgcgc cgccgctttt ttgaaaaagt tttcatcatt cattattttt gggaacattc
    29221 agaaacaatt ttcccaaatt atagagacgg ggctaaaggc agaagtatga aatttcgggt
    29281 gagccacttt cgtagctcac ccgttagcac tatcaggtat caggcggctt tcttgaggca
    29341 ggcactcata aacttattac gatcatcacc tttcagagat tgttgcgttg cttgattatt
    29401 acattcgcgc atcttttgct gctgtggcgt caaatttttt tcaccaggcg cagacttgct
    29461 gttcttcagg caatcactca tgtaggtctt acgagcatcc cctttcaacg cctgcgccgt
    29521 cgcctgctga ttacaggagg tcatacgctg ttgttgtggg gttaaggttc tctcggcagc
    29581 gccgacggtg gttaaaaaaa ccagaccaaa aagcaaggta accagtaatg ttattttcat
    29641 agcaccatcc ctcttcatgt tttaaccatg agcgtttgcg cccgtgatct gccattaagt
    29701 ctggttgcta acagcaaaaa aaccacccgg cagcgaaaat tcactgccgg gcgcggtttt
    29761 atttcagccc cagagcggct ttcatggtgt agaagagatc ggtctggtcg gtcagtccaa
    29821 caacattggc ggcatgcggg ccatacgccg caatacgcaa ctgactgccg gtatgttctt
    29881 gtgaatcctc ttcggaattc ccgtaactca tcaccatcac tgcgccatct ttagtattta
    29941 gcgcctgagt gaggcccggc gctttggtat ccggcgcaac aacctggctg gcgtgggcgt
    30001 gatcagcggt gactatgacc agcgtgttac cctccttttt agcgaattcc agcgcccgtt
    30061 gtacggcttc atcgagatcg accgtctcgc caatttgccc acaaggattc gcagcgtgat
    30121 cctgtttatc gattgacgca ccttcaactt gcaggaaaaa tcctttctca tttttactca
    30181 acaattcaat ggctttgtcg gtcatctgtg ccagggttgg tacactgtca ttacgttgcg
    30241 gattaggcgt acaggtgact gcgggcttat cgatattgcc gtggtacgtt gctttcggcc
    30301 ctagccagcg cactggcata ttgccgtcag caaacaggcc aagcaggggt ttttgctgat
    30361 tcgcttccgt caccgaattc agtgaggcag catcgttcac caactgataa ccacgcgcct
    30421 gtgcctgttc acgcagcgtt tttccctgcc attcaccagc ggttgccgtt tcagcaaagg
    30481 tttttgcgcc gccgccaagc gtaacgtcgg cacgagcgtt aagcagctgt tcggtaatcg
    30541 atccttttcc gcctttttcc agagcgttac ccggacattt ttcactggtc gcgctcggac
    30601 cgtagcattt gcgcgaggtc acatgcgcca ccagcgcagc gggcgtggca tcctgcaact
    30661 ctgcggtaga aacgttaccg gtcgccagac ctgcggcttt tgccatttcc agaatcgttg
    30721 ggtgatcttt ttcgtgaata tcgacgccca gcgcgccgtt ataggttttg acaccggttg
    30781 accaggcggt tgctgatgca gccgagtcgg tgacgtagtc cggtttgccg gtttttttat
    30841 tcagcgcata gtgagtgtat tgcccggtaa gcggtaaggc atcgatacct ttaaaaaagc
    30901 cgcccgcacc ttcggcataa ttacgtgcgg cagtaatttc cgaatccccc atcccatcgc
    30961 caatcagcaa aataatattt tttgcaggtt tatcgctaag agaatcacgc agagcggcgg
    31021 tctgatcacc cgttaagcgg cgagcaccgc cgggtgcagt aatatcgccc tgagcagccc
    31081 ggttttccag aacaggcatt tctggtgtcc gggcttttgt cacaggggta aacagtaacg
    31141 gtaagagtgc cagtgcaata gtgctttgtt tcactttatt ttctccatgt acaaatacat
    31201 taaaaaataa aaacaaagcg actataagtc tcggccgtga caactttatg acagctgttg
    31261 aaaagattaa ctttttactg acgaggatgc ttcaataact tctttacgta atcgcgcagc
    31321 agctccgtat cgtcgtcagg aatgctggca tcgggcttta cctcgtacag cgccccctct
    31381 acctgatcaa tcaaccgctg ttggtcattt tgcgccatat tgcgaagcat tgcagtgacg
    31441 ataatctcca aagcttctac ctgcgcacac agttctttcg actcttcttc tttttgggca
    31501 agcttaaata acaactcagc aatgagattt ttcatgtctg tatttcctta tccaaagtat
    31561 ggagaagtta acattatgtt tcacaacttc atagtgtctg gaagtattat t
//
LOCUS       DAOFQD010000054        31125 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.054, whole genome shotgun sequence.
ACCESSION   DAOFQD010000054 DAOFQD010000000
VERSION     DAOFQD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.054"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..221
                     /gene="tuf"
                     /locus_tag="QBF46_002425"
     CDS             <1..221
                     /gene="tuf"
                     /locus_tag="QBF46_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ6674599.1"
                     /translation="FFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            451..834
                     /gene="secE"
                     /locus_tag="QBF46_002426"
     CDS             451..834
                     /gene="secE"
                     /locus_tag="QBF46_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDQ6674600.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            836..1381
                     /gene="nusG"
                     /locus_tag="QBF46_002427"
     CDS             836..1381
                     /gene="nusG"
                     /locus_tag="QBF46_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDQ6674601.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1540..1968
                     /gene="rplK"
                     /locus_tag="QBF46_002428"
     CDS             1540..1968
                     /gene="rplK"
                     /locus_tag="QBF46_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDQ6674602.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1972..2676
                     /gene="rplA"
                     /locus_tag="QBF46_002429"
     CDS             1972..2676
                     /gene="rplA"
                     /locus_tag="QBF46_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDQ6674603.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3089..3586
                     /gene="rplJ"
                     /locus_tag="QBF46_002430"
     CDS             3089..3586
                     /gene="rplJ"
                     /locus_tag="QBF46_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDQ6674604.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3653..4018
                     /gene="rplL"
                     /locus_tag="QBF46_002431"
     CDS             3653..4018
                     /gene="rplL"
                     /locus_tag="QBF46_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDQ6674605.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4338..8366
                     /gene="rpoB"
                     /locus_tag="QBF46_002432"
     CDS             4338..8366
                     /gene="rpoB"
                     /locus_tag="QBF46_002432"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDQ6674606.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8443..12666
                     /gene="rpoC"
                     /locus_tag="QBF46_002433"
     CDS             8443..12666
                     /gene="rpoC"
                     /locus_tag="QBF46_002433"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDQ6674607.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12879..13418
                     /locus_tag="QBF46_002434"
     CDS             12879..13418
                     /locus_tag="QBF46_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HDQ6674608.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13828..14961)
                     /gene="thiH"
                     /locus_tag="QBF46_002435"
     CDS             complement(13828..14961)
                     /gene="thiH"
                     /locus_tag="QBF46_002435"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDQ6674609.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14958..15728)
                     /gene="thiG"
                     /locus_tag="QBF46_002436"
     CDS             complement(14958..15728)
                     /gene="thiG"
                     /locus_tag="QBF46_002436"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDQ6674610.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVDMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15730..15930)
                     /gene="thiS"
                     /locus_tag="QBF46_002437"
     CDS             complement(15730..15930)
                     /gene="thiS"
                     /locus_tag="QBF46_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDQ6674611.1"
                     /translation="MQILFNDQAMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15914..16669)
                     /gene="thiF"
                     /locus_tag="QBF46_002438"
     CDS             complement(15914..16669)
                     /gene="thiF"
                     /locus_tag="QBF46_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDQ6674612.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16662..17297)
                     /gene="thiE"
                     /locus_tag="QBF46_002439"
     CDS             complement(16662..17297)
                     /gene="thiE"
                     /locus_tag="QBF46_002439"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDQ6674613.1"
                     /translation="MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17297..19192)
                     /gene="thiC"
                     /locus_tag="QBF46_002440"
     CDS             complement(17297..19192)
                     /gene="thiC"
                     /locus_tag="QBF46_002440"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDQ6674614.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPAYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19273..19373)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19426..19902)
                     /gene="rsd"
                     /locus_tag="QBF46_002441"
     CDS             complement(19426..19902)
                     /gene="rsd"
                     /locus_tag="QBF46_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDQ6674615.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19997..20770
                     /gene="nudC"
                     /locus_tag="QBF46_002442"
     CDS             19997..20770
                     /gene="nudC"
                     /locus_tag="QBF46_002442"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDQ6674616.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20810..21874
                     /gene="hemE"
                     /locus_tag="QBF46_002443"
     CDS             20810..21874
                     /gene="hemE"
                     /locus_tag="QBF46_002443"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDQ6674617.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21884..22555
                     /gene="nfi"
                     /locus_tag="QBF46_002444"
     CDS             21884..22555
                     /gene="nfi"
                     /locus_tag="QBF46_002444"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDQ6674618.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22598..23188
                     /locus_tag="QBF46_002445"
     CDS             22598..23188
                     /locus_tag="QBF46_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDQ6674619.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23375..23647
                     /gene="hupA"
                     /locus_tag="QBF46_002446"
     CDS             23375..23647
                     /gene="hupA"
                     /locus_tag="QBF46_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDQ6674620.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23660..24355
                     /locus_tag="QBF46_002447"
     CDS             23660..24355
                     /locus_tag="QBF46_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDQ6674621.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24357..24776)
                     /gene="zraP"
                     /locus_tag="QBF46_002448"
     CDS             complement(24357..24776)
                     /gene="zraP"
                     /locus_tag="QBF46_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDQ6674622.1"
                     /translation="MKRNTKISLVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            25014..26390
                     /gene="zraS"
                     /locus_tag="QBF46_002449"
     CDS             25014..26390
                     /gene="zraS"
                     /locus_tag="QBF46_002449"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDQ6674623.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPVIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAAGQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLCLPVN
                     ITRKDPQG"
     gene            26387..27712
                     /gene="zraR"
                     /locus_tag="QBF46_002450"
     CDS             26387..27712
                     /gene="zraR"
                     /locus_tag="QBF46_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDQ6674624.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLCGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTIAVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27709..28998)
                     /gene="purD"
                     /locus_tag="QBF46_002451"
     CDS             complement(27709..28998)
                     /gene="purD"
                     /locus_tag="QBF46_002451"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDQ6674625.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTIERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29010..30599)
                     /gene="purH"
                     /locus_tag="QBF46_002452"
     CDS             complement(29010..30599)
                     /gene="purH"
                     /locus_tag="QBF46_002452"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDQ6674626.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAKTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKLDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7334 a         8047 c         8513 g         7231 t
ORIGIN      
        1 cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg actggtacca
       61 tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa atggttgtta
      121 ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt gaaggcggcc
      181 gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata acatttgacg
      241 caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt accagaacct
      301 ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt tgagggcgta
      361 taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg gggtgaaaat
      421 gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc tcaaggaagc
      481 gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct ggtggcgatt
      541 gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc cgtagtaatt
      601 ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc taccgttgct
      661 tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg ccaggaaaca
      721 ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat cctgtgggga
      781 ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt ctgagatgtc
      841 tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg aaggccgcgt
      901 agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt ttggtgaagt
      961 catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca aaagcgaacg
     1021 taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga gctggcacct
     1081 ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc gtcctgcgcc
     1141 aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg gtgataagcc
     1201 gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg gtccgttcgc
     1261 tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga aagtgtctgt
     1321 ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg aaaaagccta
     1381 acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat tggaatacaa
     1441 tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata tcacggggag
     1501 cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa agtacaagcc
     1561 tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt aggtccggct
     1621 ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc aaaaactgat
     1681 tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga ccgttctttc
     1741 actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc tggtatcaag
     1801 tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc tcagctgcag
     1861 gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc gatgactcgc
     1921 tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga aatggctaaa
     1981 ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca gtacgacatc
     2041 aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga aagcgtggac
     2101 gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg tggtgcaact
     2161 gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca aggtgcaaat
     2221 gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct ggctgaccag
     2281 atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc aatgcgcgtt
     2341 gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc gaaagtgggt
     2401 actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca ggttcgttac
     2461 cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt tgacgctgac
     2521 aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa accgactcag
     2581 gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc aggtgttgca
     2641 gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg cggtgatttt
     2701 gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga gatattcggg
     2761 ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg tgactggtgc
     2821 gaatgaagga agccaacgtc gccacaagtt gaatagcgac ggatagaaaa gatttgttcg
     2881 ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca agaaacttaa
     2941 tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg gcttgtttct
     3001 gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc cagagatttt
     3061 ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca aacaagcgat
     3121 tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg cggattcccg
     3181 tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag ctggcgtata
     3241 catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc cgttcgagtg
     3301 cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac acccgggcgc
     3361 tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg tcaaagccgc
     3421 tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa ctctgccgac
     3481 ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg ctggcaaact
     3541 ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc agttatcttt
     3601 ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt aaatgtctat
     3661 cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg ttgtagaact
     3721 gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag ctgtagctgc
     3781 tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga aagctgctgg
     3841 cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg gtctgaaaga
     3901 agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga gcaaagacga
     3961 cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag ttaaataagc
     4021 caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt ttagtcacca
     4081 gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact gctgtgcctt
     4141 tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt tctgtgtaaa
     4201 tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt tccattttcc
     4261 ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt cgacttgtca
     4321 gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt cgtaaggatt
     4381 ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag cttgactcgt
     4441 ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct gctttccgtt
     4501 ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc agctaccgcc
     4561 ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc tattccgcac
     4621 cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa ggcaccgtaa
     4681 aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca gacaacggta
     4741 cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt agtccgggcg
     4801 tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg tataacgcgc
     4861 gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag gacaacctgt
     4921 tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc gccctgaact
     4981 acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa atccgtgata
     5041 acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca tcttttgaca
     5101 tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg cgccacattc
     5161 gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac atcgcaggta
     5221 aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc gcagcgaaca
     5281 tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag cgtatcgaaa
     5341 cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta cgtgtcgacc
     5401 caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc cctggcgagc
     5461 cgccgactcg tgaagcagcg gaaagcctgt tcgagaacct gttcttctcc gaagaccgtt
     5521 atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc gaagaaatcg
     5581 aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag ctcatcgata
     5641 tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt cgtatccgtt
     5701 ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta gagcgtgcgg
     5761 tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat atgatcaacg
     5821 ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg tctcagttta
     5881 tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc gcactcggcc
     5941 caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac ccgactcact
     6001 acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg atcaactctc
     6061 tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat cgtaaagtga
     6121 ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa ggcaactacg
     6181 ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa gacctggtaa
     6241 cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac tacatggacg
     6301 tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg gaacacgatg
     6361 acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg actctgcgtg
     6421 ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac tccggtgtaa
     6481 ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt atcgttatca
     6541 aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac aacctgacca
     6601 aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg tctctgggtg
     6661 aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc ggtgaactgg
     6721 cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac ttcgaagact
     6781 ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc cacattcagg
     6841 aactggcgtg tgtgtcccgt gacaccaagc tggggccaga agagatcacc gctgacatcc
     6901 cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt tatattggtg
     6961 cggaagtgac cggtggcgac attctggttg gtaaggttac gccgaaaggt gaaactcagc
     7021 tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct gacgttaaag
     7081 actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt caggtcttta
     7141 ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg cagctcaaac
     7201 aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg ttcagccgta
     7261 tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa ctgccgcgcg
     7321 atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg gaacagctgg
     7381 ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg aaacgccgca
     7441 aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag gtatatctgg
     7501 cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt aacaagggtg
     7561 taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt acgccggtag
     7621 acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag atcctcgaaa
     7681 cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg ctgaaacagc
     7741 agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg ggcgctgacg
     7801 ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt ctggctgaaa
     7861 acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa gaagcagaaa
     7921 ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc ctgtacgatg
     7981 gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac atgctgaaac
     8041 tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac agcctggtta
     8101 ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg gagatggaag
     8161 tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc accgttaagt
     8221 ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc aaccatcaga
     8281 tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt tcgctgggta
     8341 tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct gtcgggttaa
     8401 aactcggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga tttattaaag
     8461 tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc tctggcttcg
     8521 ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac catcaactac
     8581 cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg gccggtaaaa
     8641 gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt catctgtgag
     8701 aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg ccacatcgaa
     8761 ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg tatcggtctg
     8821 ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc ctatgtggtt
     8881 atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga gcagtatctg
     8941 gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga agcaatccag
     9001 gctctgctga agagcatgga tctggagcaa gagtgcgaac agttgcgtga agagctgaac
     9061 gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact gttggaagcg
     9121 ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc ggtactgccg
     9181 ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc tgacctgaac
     9241 gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct ggatctggct
     9301 gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt tgacgccctg
     9361 ctggataacg gtcgtcgcgg tcgtgcaatc accggttcta acaagcgtcc tctgaaatct
     9421 ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctact cggtaagcgt
     9481 gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct gcatcagtgc
     9541 ggtctgccga agaaaatggc actggagctg ttcaaaccgt tcatctacgg caagctggaa
     9601 ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg cgaagaagct
     9661 gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct gaaccgtgca
     9721 ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga aggtaaagct
     9781 atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg tgaccagatg
     9841 gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct gatgatgtct
     9901 accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc tcaggacgtt
     9961 gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga aggcatggtg
    10021 ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc tctgcatgcg
    10081 cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt agtagcgaaa
    10141 accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt accgaaaggt
    10201 ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa aatgctgaac
    10261 acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca gatcatgtac
    10321 accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga catggtcatc
    10381 ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga aattcaggag
    10441 cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat cgatatctgg
    10501 gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac tgaaaccgtg
    10561 attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta catgatggcc
    10621 gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat gcgtggtctg
    10681 atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt ccgtgaaggt
    10741 ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct ggcggatacc
    10801 gcactgaaaa ctgcaaactc cggttacctg actcgtcgtc tggttgacgt ggcgcaggac
    10861 ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac tccggttatc
    10921 gaaggtggtg atgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt aactgctgaa
    10981 gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct gctgcacgaa
    11041 cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg ttctgttgta
    11101 tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct ggcgcgtggc
    11161 cacatcatca acaagggtga agcaatcggt gttatcgcgg cacagtccat cggtgaaccg
    11221 ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg tgcggctgct
    11281 gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt gaagtcggtt
    11341 gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa actgatcgac
    11401 gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact ggcgaaaggc
    11461 gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca caccatgccg
    11521 gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg tcagaccatt
    11581 actcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga ttccgcagaa
    11641 cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc tcagggtaac
    11701 gacgttctga ttccgggtac cgatatgcct gcgcagtact tcctgccggg taaagcgatt
    11761 gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg tattccgcag
    11821 gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga cctgttcgaa
    11881 gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt ttccttcggt
    11941 aaagaaacca aaggtaaacg tcgtcttgtt atcaccccgg tagacggtag cgatccgtac
    12001 gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg tgtagaacgt
    12061 ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct gcgtggtgtt
    12121 catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct gcagggcgtt
    12181 aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa agctaccatc
    12241 gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc tcgcgtcaag
    12301 atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc ccgcgatctg
    12361 ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc atcgttccag
    12421 gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga actgcgcggc
    12481 ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta cgcgtaccac
    12541 caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca ggtgactgca
    12601 gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg ttctgataac
    12661 gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt tttatggggg
    12721 gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac tcataatcaa
    12781 tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact cggcaaggat
    12841 tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa gtggaaaagt
    12901 ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt gatgctcatc
    12961 aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc ttttgggtta
    13021 cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa gaaaaacaat
    13081 cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt tataacttag
    13141 cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt attcatgccg
    13201 ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag gcattttact
    13261 ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta actagcgctt
    13321 atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt gcctacaact
    13381 tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct taaggtgggt
    13441 ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc cttgaccttg
    13501 cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc tggaagatga
    13561 gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct tacaatcaga
    13621 tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat ttttaagaaa
    13681 ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta
    13741 ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg
    13801 aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc ccagatagct
    13861 gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc
    13921 cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc
    13981 acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc
    14041 gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg
    14101 gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc
    14161 gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg
    14221 ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag
    14281 gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt
    14341 ttccagccgc cagaagaagt cctgtttttt tcctttcaga tgatggcggg cataagtcgc
    14401 ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct ttaactcggc
    14461 gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag
    14521 ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac
    14581 taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc
    14641 ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc
    14701 gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg
    14761 ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac tggcggctgg
    14821 cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac
    14881 gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca
    14941 gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca
    15001 tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc
    15061 gccagacgaa atgccttcgc catgtcgacg ggatcgtccg cgacggcaat tgccgtattc
    15121 actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg aacgccgatg
    15181 ccagcatcga caaccaccgg cactgtggcc tgctggataa taatctccag catggcgcgg
    15241 gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg
    15301 acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat
    15361 ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg caacagccag
    15421 cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgagccaga
    15481 tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg cagcagggtc
    15541 acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt
    15601 ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt
    15661 gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata
    15721 cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac
    15781 gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc
    15841 cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg
    15901 catcgcttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg
    15961 cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc
    16021 ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca
    16081 taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt
    16141 tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact
    16201 gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc acgcaggcgg
    16261 cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg
    16321 caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatgt
    16381 cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga tcgatatctt
    16441 cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca
    16501 gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta cccagcccgc
    16561 ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat
    16621 cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct
    16681 gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg
    16741 acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc cagactgata
    16801 ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc
    16861 agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg
    16921 atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg
    16981 cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc
    17041 cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac
    17101 aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct
    17161 tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt
    17221 tcgatccact gtacgctgtc caccaccggg tacagtcctg aacgaaaagg tacaggagga
    17281 aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcac
    17341 ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg gcgtaatcac
    17401 gcacttcctg gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg
    17461 cgactttacc tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt
    17521 cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt
    17581 tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct
    17641 tataggtgat aagcccctgc ttaacatctt ctttattggg cagacccaga tgctcttttg
    17701 gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg
    17761 acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg
    17821 gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca
    17881 cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt
    17941 cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg
    18001 ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt
    18061 cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa
    18121 tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca
    18181 gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg
    18241 tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct
    18301 ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt
    18361 cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc
    18421 ataccagctt ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg
    18481 cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa
    18541 tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga
    18601 tattttccgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacttcgc
    18661 tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggtgtga
    18721 tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgttttg gcgcgttttg
    18781 gcgttaataa gccgctaaaa cgtagctcgt cgaggccatc atctgccagc cgcgctttag
    18841 tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccatggct
    18901 ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatcg ccatacgggc
    18961 cggaggtgtc gtaggccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctgc
    19021 cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccgggttgtg
    19081 tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa
    19141 aatgttgggc ctgggcgcgc tgttcgcggc gggtcagttt tgttgcagac atagctcatt
    19201 ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa
    19261 aggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc
    19321 cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc
    19381 acaaacgggg cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt
    19441 gacgcgggcg gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc
    19501 cagcgcctcg ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat
    19561 ggcggtttcc aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg
    19621 cggccaaatc ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg
    19681 ctcataaata ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc
    19741 aagggctttt tcgtttagcc tcatgtacga ttctttgcca ggcttaatgc caaccagatt
    19801 gtagtaagcc acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact
    19861 tcctctgacg cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt
    19921 gtattcaaaa actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa
    19981 aggtagtgca agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt
    20041 cagccatgaa caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa
    20101 tttcgatctt gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg
    20161 gttagtacaa cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga
    20221 tgttgggctg tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca
    20281 taaatactgt ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct
    20341 gtgcagccat tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat
    20401 ccgtcgcgat gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac
    20461 ggtacttgcc ggattcgtcg aagtgggcga aaccctcgag caggcggtcg cgcgggaagt
    20521 gatggaagag agcggaatta aagttaaaaa cttgcgttat gtgacttccc agccgtggcc
    20581 gtttcctcag tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat
    20641 cgacccgaaa gaattgcttg aggcgaactg gtatcgctat gacgatttgc cgttactccc
    20701 gccgcccggc accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga
    20761 gtatgagtga tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact
    20821 taaaaacgat cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg
    20881 gatgatgcgc caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg
    20941 cgattttatg tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct
    21001 gcgtcgctac ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc
    21061 gatggggtta gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac
    21121 ctgcaaagcc gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt
    21181 gatgaacgcg gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt
    21241 ttccggcagc ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt
    21301 caccgtgatc aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa
    21361 actggcgaaa agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt
    21421 gatgattttc gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct
    21481 ctattacatg cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtacc
    21541 ggtcacgctg tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg
    21601 cgatgcgttg ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa
    21661 taaagtcgcg ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat
    21721 tgaagaagaa gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa
    21781 ccttggtcac ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc
    21841 agtgcatcga ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt
    21901 acgcgctcaa caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga
    21961 tccaccggat ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg
    22021 agcggcgatg gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg
    22081 catcgccacc accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct
    22141 gctggcagcg tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg
    22201 gatctcgcat cctcgccgtc ttggcgtcgc cagccatttt ggcttattgg tggatgtgcc
    22261 gaccattggc gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc
    22321 gggcgcgctg gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa
    22381 agcgcgctgt aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct
    22441 ggcgtgggta caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga
    22501 cgcggtggcc tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag
    22561 gtaaactgcg gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct
    22621 gcgtctggag cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg
    22681 catgtacccc aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta
    22741 ccgtcgcatt ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt
    22801 cgacagccaa ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta
    22861 cggcgtttat ccggcaatcg atgcatgcgt ggcgttaagt gaactggtcc attcgcgttt
    22921 gagtggtgaa acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc
    22981 gatgctggaa atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc
    23041 agctgtagag caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga
    23101 agaacgcgat atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag
    23161 caatattggt ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt
    23221 cgtacctgga gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag
    23281 tggctatcgg tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc
    23341 ttagcaagcg ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt
    23401 aattgcagag aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct
    23461 ggctgcaatt actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac
    23521 cttcaaagtg aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat
    23581 caaaattgcc gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt
    23641 taagtaagat tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc
    23701 tttctggcgt cgatcattga tgctggcagg tgcgctgttt ctcactgcct gtagccacaa
    23761 ctcttcactt cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat
    23821 ctggcgaaaa gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag
    23881 tggcgatacc acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat
    23941 caatgtttac agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct
    24001 gagctttatg caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga
    24061 tttataccgt tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag
    24121 agtcgtgctg cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg
    24181 gcaaaccatt aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag
    24241 ccgctcttct gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact
    24301 gttggtggca aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac
    24361 cagtggccca tacccatatg accgccacca ccgcagccgc cgtaacccat tccggtaccg
    24421 cgcggaatac ccgcttcagc catcgcaata tctcgtttca cccgtaactc atctaacgac
    24481 tgacgcaaat tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc
    24541 gcggctaaca gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg
    24601 ctttgagcgt aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa
    24661 ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgagccc
    24721 atcgccattg ctgaaagcgc catcattacc agggaaattt tcgtgttccg tttcatggtt
    24781 aatcctccga tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct
    24841 gatatgacgg gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc
    24901 gagtcatttt tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat
    24961 aaaagatggc atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt
    25021 ttatgcaacg ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca
    25081 ttgttgggct ggtggggctg tttgcggtga cggtgattcg cgattatggg cgcgagactg
    25141 ccgccgccag acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg
    25201 gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag
    25261 aaatggccgg gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg
    25321 tgatgcatag caactccggc atggtgggaa aacagcttta ttccccgcag gaaatgcagc
    25381 agttacatcc gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg
    25441 ttctggaaat ttatcgccag tttcaaccga tgtttgctgc tggaatgcac cggatgcgcc
    25501 atatgcagca gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata
    25561 ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat tatcctcttc gccctggcga
    25621 cggtcttgct ggcaagcgtg ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc
    25681 agctgttgca ggatgaaatg aagcgcaaag agaagctggt ggcactgggg catcttgcag
    25741 caggcgttgc ccacgaaatc cgtaatccac tttcctcaat taaagggctg gcgaaatact
    25801 ttgccgaacg cgcgccagca gggggagaag cgcatcaatt ggcgcaggtg atggcgaaag
    25861 aagccgaccg tttaaaccgt gtggtaagcg agttgctgga actggttaag ccaacgcatc
    25921 tggctttgca ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc
    25981 aggatgcaaa cagccgggag atccagttac gctttaccgc caacgacaca ttaccggtaa
    26041 ttcaggccga cccagacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc
    26101 aggcgattgg tcagcatggt gtgattagcg tgacggccag cgaaagcggc gcgggcgtga
    26161 aaatcagcgt taccgacagc ggtaagggaa ttgcggcagg tcagcttgaa gccatcttca
    26221 ctccgtactt caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata
    26281 ttgttgaaca acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggcgcaacgt
    26341 tcaccctctg tcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa
    26401 tatcgatatt ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact
    26461 gtgcggctgg ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt
    26521 gcgggagcga gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat
    26581 tgccacgcta aaagagatca aagcgttaaa tccggcgatt ccggtactga ttatgaccgc
    26641 atactccagc gtcgagacgg cggtagaggc actgaaaacc ggggcgctgg attatctcat
    26701 caagccgctg gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca
    26761 cagtattgat gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag
    26821 cccggcgatg caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt
    26881 gctgatccac ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag
    26941 tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt
    27001 gctggaatct gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg
    27061 ggaagggcgc tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat
    27121 ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt
    27181 cggtagcaac cagactatcg cggttgatgt ccggctgatt gcggcgaccc atcgcgatct
    27241 tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt
    27301 ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca
    27361 ttttctgcag cgctttgccg agcgtaatcg caaggcggta aaaggtttta cgccccaggc
    27421 gatggatctg ttgattcatt acgactggcc gggaaatatt cgtgagctgg aaaacgcggt
    27481 ggaacgggca gtggtgctgc tgactgggga atatatttcc gaacgcgagc tgccgctggc
    27541 gattgccagt acgccgatcc cgctgggaca aagtcaggat attcagccgt tggtggaagt
    27601 ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc
    27661 ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc
    27721 gcgttcgatg gcgcgccagc cgatatcttt ccggcagaag cagtcgtccc agtgaatatc
    27781 ggtcattaag gcataagcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac
    27841 gcacagtacg cgcccgccgc tggtcactac ctgctcgtca tccgccagtt ttgtgcctgc
    27901 atggaacact ttgccgtctt ccacttcttc cagcggcagg ccgtggatca catcaccggt
    27961 gcggtaatcg cccggatatc cacccgcagc catcaccacg ccgagagaag cgcgttcatc
    28021 ccactcggat gttttctcgt ccagcttccc ttcacaggct gccaggcaga gctcaaccag
    28081 atcggacttc atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa
    28141 ttcgataacc ttcggattgc cctgtttgtc gatcatcagg cccgcgtaga gaaaaccggt
    28201 gtaggtgttg ccttccgccg ccatgccttt cacggttggc cagatgatac gttctatggt
    28261 gcgctgatga acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt
    28321 gtttggtccg gtatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag
    28381 cacatgctct ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc
    28441 gataacgatg cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc
    28501 cgcttccgct tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc
    28561 cgctttaatg acgattggcg cgcctttctc acgcagatac gccagcgcag gttctacctc
    28621 ggtgaagttc tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa
    28681 cgcttttgag ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc
    28741 ggcgcggaag gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag
    28801 atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg atatcggtta cgccgatagc
    28861 gacgttctgt agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt
    28921 ttcaaccagc ggcgactgcg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc
    28981 aatcactaat actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag
    29041 agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatagaaccg
    29101 ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac
    29161 gggaagaacg cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggccgct
    29221 ttaataccgg cgatttttgc ggagtacacg cggctcatct ggccggcgcc aatgccgata
    29281 gtcatattgt ttttggcata gacgatagcg ttggatttca caaacttcgc caccttccag
    29341 cagaacagcg catcacgcag ttcctgttcg ctcggctgac gtttggtcac cacgcgcagt
    29401 tcttccgcac cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt
    29461 ttgaaatcga ggcccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc
    29521 tgtttggcgg cggtgatttt cagggcttct tcgctggcgg acggcgcaat aatcacttcg
    29581 acaaactgac gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca
    29641 atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga
    29701 atggaattgc cgatagccac gccgcaaggg ttggcgtgct tcacaatcac acatgccggt
    29761 tcggcgaact ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag
    29821 agggctttac cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct
    29881 atatagaagg cagcctgctg gtggctgttc tcgccgtaac gcatatccag cttcttaatg
    29941 aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtaa
    30001 gccggaacca tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg
    30061 aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc
    30121 atctctttaa taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg
    30181 ttcttggcgg cggagcgcac catcgttggg ccgccgatat cgatgttctc aaccgcatct
    30241 tccagcgagc aaccttcacg ggcaacggtc ttggcgaacg gatacaggtt aacaaccacc
    30301 atatcgatag gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc
    30361 agaatgccac catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg
    30421 aaaccggtgt aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca
    30481 gtgccccctg tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg
    30541 ataccggctt tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg
    30601 gtaaatcccc tggatttgac tattacagag agcgttagct gaatttttcg cgaaaaactc
    30661 agctaacgcc cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc
    30721 gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg
    30781 ggaaaaatac agaaattaca ttggtgattg tggataactc tgtgcgtaaa aaggtataaa
    30841 gcgggctttt gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc
    30901 ctgcggagaa ctccctataa tgcgcctcca tcgacacggc ggatgtgaat cacttcacac
    30961 aaacagccgg ttcggttgaa gagaaaaatt ctgaaattca gggttgactc tgaaagagga
    31021 aagcgtaata tacgccacct cgcgacagtg cgctaaagcg tgtcgcaact gctctttaac
    31081 aatttatcag acaatctgtg tgggcactcg aagatacgga ttctt
//
LOCUS       DAOFQD010000055        31100 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.055, whole genome shotgun sequence.
ACCESSION   DAOFQD010000055 DAOFQD010000000
VERSION     DAOFQD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.055"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..248)
                     /locus_tag="QBF46_002453"
     CDS             complement(<1..248)
                     /locus_tag="QBF46_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDQ6674627.1"
                     /translation="MELKKLMEHISITPDYRQAWKVVHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSQGK"
     gene            complement(474..1043)
                     /locus_tag="QBF46_002454"
     CDS             complement(474..1043)
                     /locus_tag="QBF46_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674628.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQDKDHDCFTIFAKGTIFSSFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLIKIQHELIRSEEK"
     gene            complement(1040..5239)
                     /locus_tag="QBF46_002455"
     CDS             complement(1040..5239)
                     /locus_tag="QBF46_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDQ6674629.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPIGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDITRY
                     RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHRE
                     EGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWYYDEADRLTHRTVKGETAEQWQYDERGWLTDISHISEGHRVAV
                     HYGYDSKGRLASEHLTVHHPQTNELLWQHETRHAYNAQGLANRCIPDSLPAVEWLAYG
                     SGYLAGMKLGDTPLVDFTRDRLHRETLRSFGRYELTTAYTPAGQLQRQHLNSLQYDRD
                     YTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPE
                     LHPDSALSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQ
                     HRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWD
                     GDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLANTLQQSGGEDGGS
                     VVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLY
                     HCDHRGLPLALISTEGTTAWYAEYDEWGNQLNEENPHQLQQLIRLPGQQYDEESGLYY
                     NRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAR
                     PNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPN
                     LTCTPQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAG
                     IDDLGEILAKMKQRTSRGIRK"
     gene            complement(5399..5611)
                     /gene="rpmE"
                     /locus_tag="QBF46_002456"
     CDS             complement(5399..5611)
                     /gene="rpmE"
                     /locus_tag="QBF46_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDQ6674630.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            5814..8012
                     /gene="priA"
                     /locus_tag="QBF46_002457"
     CDS             5814..8012
                     /gene="priA"
                     /locus_tag="QBF46_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDQ6674631.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8168..9193
                     /gene="cytR"
                     /locus_tag="QBF46_002458"
     CDS             8168..9193
                     /gene="cytR"
                     /locus_tag="QBF46_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDQ6674632.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKHQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9285..10244
                     /gene="ftsN"
                     /locus_tag="QBF46_002459"
     CDS             9285..10244
                     /gene="ftsN"
                     /locus_tag="QBF46_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDQ6674633.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASSQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            10337..10867
                     /gene="hslV"
                     /locus_tag="QBF46_002460"
     CDS             10337..10867
                     /gene="hslV"
                     /locus_tag="QBF46_002460"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDQ6674634.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            10877..12208
                     /gene="hslU"
                     /locus_tag="QBF46_002461"
     CDS             10877..12208
                     /gene="hslU"
                     /locus_tag="QBF46_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDQ6674635.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            12275..13201
                     /gene="menA"
                     /locus_tag="QBF46_002462"
     CDS             12275..13201
                     /gene="menA"
                     /locus_tag="QBF46_002462"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDQ6674636.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13294..13779
                     /gene="rraA"
                     /locus_tag="QBF46_002463"
     CDS             13294..13779
                     /gene="rraA"
                     /locus_tag="QBF46_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDQ6674637.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(13864..14109)
                     /gene="zapB"
                     /locus_tag="QBF46_002464"
     CDS             complement(13864..14109)
                     /gene="zapB"
                     /locus_tag="QBF46_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDQ6674638.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            14534..15379
                     /gene="glpF"
                     /locus_tag="QBF46_002465"
     CDS             14534..15379
                     /gene="glpF"
                     /locus_tag="QBF46_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDQ6674639.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15402..16910
                     /gene="glpK"
                     /locus_tag="QBF46_002466"
     CDS             15402..16910
                     /gene="glpK"
                     /locus_tag="QBF46_002466"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDQ6674640.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            17045..18055
                     /gene="glpX"
                     /locus_tag="QBF46_002467"
     CDS             17045..18055
                     /gene="glpX"
                     /locus_tag="QBF46_002467"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDQ6674641.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            18152..18898
                     /gene="fpr"
                     /locus_tag="QBF46_002468"
     CDS             18152..18898
                     /gene="fpr"
                     /locus_tag="QBF46_002468"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDQ6674642.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPHCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESTIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(18903..19331)
                     /gene="uspD"
                     /locus_tag="QBF46_002469"
     CDS             complement(18903..19331)
                     /gene="uspD"
                     /locus_tag="QBF46_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDQ6674643.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(19358..19657)
                     /locus_tag="QBF46_002470"
     CDS             complement(19358..19657)
                     /locus_tag="QBF46_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDQ6674644.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNNNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(19869..20309)
                     /locus_tag="QBF46_002471"
     CDS             complement(19869..20309)
                     /locus_tag="QBF46_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDQ6674645.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            20410..21009
                     /locus_tag="QBF46_002472"
     CDS             20410..21009
                     /locus_tag="QBF46_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDQ6674646.1"
                     /translation="MKPGCTLFFLLCSALTVTTAAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            21117..21884
                     /gene="tpiA"
                     /locus_tag="QBF46_002473"
     CDS             21117..21884
                     /gene="tpiA"
                     /locus_tag="QBF46_002473"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDQ6674647.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(21939..22694)
                     /gene="cdh"
                     /locus_tag="QBF46_002474"
     CDS             complement(21939..22694)
                     /gene="cdh"
                     /locus_tag="QBF46_002474"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDQ6674648.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(22800..23789)
                     /gene="sbp"
                     /locus_tag="QBF46_002475"
     CDS             complement(22800..23789)
                     /gene="sbp"
                     /locus_tag="QBF46_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDQ6674649.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(24109..25071)
                     /gene="pfkA"
                     /locus_tag="QBF46_002476"
     CDS             complement(24109..25071)
                     /gene="pfkA"
                     /locus_tag="QBF46_002476"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDQ6674650.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(25252..26154)
                     /gene="fieF"
                     /locus_tag="QBF46_002477"
     CDS             complement(25252..26154)
                     /gene="fieF"
                     /locus_tag="QBF46_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDQ6674651.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(26303..26803)
                     /gene="cpxP"
                     /locus_tag="QBF46_002478"
     CDS             complement(26303..26803)
                     /gene="cpxP"
                     /locus_tag="QBF46_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDQ6674652.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            26953..27651
                     /gene="cpxR"
                     /locus_tag="QBF46_002479"
     CDS             26953..27651
                     /gene="cpxR"
                     /locus_tag="QBF46_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDQ6674653.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            27648..29021
                     /gene="cpxA"
                     /locus_tag="QBF46_002480"
     CDS             27648..29021
                     /gene="cpxA"
                     /locus_tag="QBF46_002480"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDQ6674654.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALGRMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(29127..29801)
                     /gene="yiiM"
                     /locus_tag="QBF46_002481"
     CDS             complement(29127..29801)
                     /gene="yiiM"
                     /locus_tag="QBF46_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDQ6674655.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(29950..30933)
                     /gene="kdgT"
                     /locus_tag="QBF46_002482"
     CDS             complement(29950..30933)
                     /gene="kdgT"
                     /locus_tag="QBF46_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDQ6674656.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALWHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARVAKIEILGTVK"
BASE COUNT         7420 a         8212 c         8047 g         7421 t
ORIGIN      
        1 atctttccct gggatacaac tctggcaatg gtatcgtgaa caggaatacc attttcaaaa
       61 tcaccatatt gcttcaaaaa atcgagatgt gtttccccga aatcctctat atcttcccaa
      121 ccttctgcac cagaaataac ggcacaaata gtcaacagta gaatatctga caatttatgc
      181 accactttcc aggcttgtct gtaatcgggg gtaatagaaa tatgttccat caatttttta
      241 agttccattt gttcacctta tttatgtaag gagtatttga tcatgtataa gctatgaaaa
      301 acagctttag acaataagta gtatctcaat tgttgaactt aaaattcgaa ttatttagta
      361 caaaaaagta gcgttacgtg atctttccct gcaaatggaa cgcaaaataa acacctattc
      421 catgagttat ataatttttc acaatgatta atttgttttc tcaggaagag attttatttc
      481 tcttcactac gaattagttc atgctgtatc tttattaaac attcgtaaga ctcatctttg
      541 ttcgggagat gtgctgtcat tcgagcataa gttggcttct catctttaaa atatttaacc
      601 atgattgaat tatcaaggac tgccaacaac tgagctggat cactgctctt tttttgatag
      661 atacccgctc ttatataact tctaaaccga tcgccgagaa acgccgtttt ttctgctgta
      721 tttatcaact cctgcaacgt tcctttgatg gcatgatttt cattaaggcg tagtgaccca
      781 acgcgaatta agagtctcgc cccgcttggt gtgaatttat tatttttaca tgtgcccagt
      841 tctgtttgcc aataatactc accgatgtcc tcattctgat accatctcca catggcttta
      901 ttatttaaga caggaaacga ggaaaaaata gtacccttag cgaaaatagt aaaacaatca
      961 tgatctttat cttgtatttc gcaatcagct ataacaccaa aactaacaaa tatcatcagc
     1021 aaaaagaaca aaactctttt cattttctta ttcctctcga tgttctctgt ttcatcttag
     1081 ctaaaatttc acccaaatca tctatccctg ctgttgctgg cccatcgggt ttattaatgt
     1141 ctgggaataa atagggttct gcacaagtat atcctggtgg taattgagtt gttggccaat
     1201 ttgtctgtgg caggtaatca cttgctgtct ttgtatccat ccctggatac tgggaatcat
     1261 cctgtggcgt acaagtcaga tttggtttta cccacttcct gcaatacagt gtgtagtcgt
     1321 aagggcttaa tgctctgaag aatgttttga aaacattagg cgcactactg gcctggtcaa
     1381 taggatatgg cagtcttaat tgtttcgcta tgtccggcaa tttacttgga tcgggcgtag
     1441 gcggtttgtt gggtctcgct ccatatccgc cattatttaa gtgtccatat tttgatgcca
     1501 gtcccattga atctatatac tgaaccggat tcagcggata ctgataaaaa ttccatcccc
     1561 ccttcagccc aatcggatcc tgagtgatat acctcccctg cagcgggtca taatagcggt
     1621 ggcggttgta atacaggccg gactcctcat catactgctg ccccggcagg cggatgagct
     1681 gctgcagctg gtgcgggttc tcttcattca gctggttacc ccattcatcg tattctgcgt
     1741 accacgctgt tgtcccttcc gtgctgataa gggccagcgg caggccgcga tggtcgcagt
     1801 ggtacaggtg aatttttcgc gccggcgtgt ataccgggtc catctggctt tgcatctgcg
     1861 ccaccgtcag gccgcacgat gccagccagc ggcggctttc ctcactcacc cggtcagcca
     1921 ggatttcact ttccagccgg tcgagcatct gcaccagcac cggcgggaac accacactgc
     1981 caccgtcttc gccgccggac tgctgaaggg tattcgccag gctgcggcgc tgcgttttcg
     2041 ccagctcacc ggtggcggtt tcaactctga tgagtggcgt gaagctcccc ggctgataaa
     2101 tcgtctggat gcgggttctg tcgttctgta ttgtggtcag ccggtcgccg tcccagccgt
     2161 accaggtcac ttgcggtttc cgtgacagcg acatccagcc cgtcaggtcc cgttcacgcc
     2221 gccatacccg ttttgccacc ctgcggccca gcgggtcgta aagatagcga ctttcgacca
     2281 gcggctcttc atattgtgtc cgcgtgtagt gcaccagccg gtgctgactg tcgtaatggt
     2341 accggtgagt ccgctcatca tccgtgcgga taaccccttc cgggatgagg tcggttttct
     2401 ctgtcagcct gccgtgacgg tcataccggt aaagatagtg cgcgtcacgg gcgatacggt
     2461 tatccggcca catgctgagg gcgctgtccg ggtgcagctc cgggtcgggc aggcggttac
     2521 ctgccgggtc tgtggcatac gggatgcgga tatccagatt cgctgcggtg gtgtgaacgc
     2581 cggtcagcct gccggtggtg ctgtagctgt aactccgggt ctggcgcggg ctgctgatgc
     2641 ggatgagttc gccgttgtcg ttccaggtgt aatcgcggtc atactgcagg ctgttcaggt
     2701 gctgacgctg taactgcccg gcaggggtgt aagcggtggt gagttcataa cgaccgaagc
     2761 tgcgcagcgt ttcccggtgc aggcggtcgc gggtgaaatc caccagcggt gtgtcgccga
     2821 gcttcatgcc tgccaggtaa ccgctgccgt aggccagcca ttccacggcg ggcaggctgt
     2881 ccggtataca gcggttcgcc agtccctgtg cgttgtacgc atgtctggtc tcatgctgcc
     2941 agagcagttc attcgtctgc ggatgatgca ccgtcaggtg ttcactggcg aggcggcctt
     3001 tactgtcata accatagtgc accgccaccc ggtgcccttc gctgatatgg ctgatgtctg
     3061 tcagccagcc gcgttcgtca tactgccact gctctgcggt ttcacccttc acggtgcggt
     3121 gagtgaggcg gtctgcttcg tcatagtacc agtgggtgac cagcccctca tcctcgctgc
     3181 ggataagttt gccggtcagg tcgtggtgat aacgctgtgt gcggccgtca aagccggttt
     3241 cctgtatcag ccggtcgagt acatcgtaac ggaaggtggt gtggctgccg ttttcactgg
     3301 tcaggcggat gacccgtccg gcagcatcgt attccatact gcgcgtcagc ccgccctgcg
     3361 tggtacagat ggcctttccc cacgcatcgt actgtgtccc gtttctgctg ccgtccgggg
     3421 caatgacggt ggtcaggtca ccggcggcgt tgtattcata ccgcgtttca tggccctgcg
     3481 tgtctttcac ggcaattaac tgtccacggc tgtcgtatgc gcggtactgg ctcaggcctt
     3541 cctcgcggtg caccgccgtc acctgaccaa aacggtcatg gtcataacgg gttacataac
     3601 cggaacagtc ggtgaagctc agcaactggc cgtaacggct ccacgtcatg gttttccggc
     3661 tgccggtggc atcttccgtt gcgcagggta agtcactgtg tggattatca taacggtagc
     3721 gggtgatatc gccgtcaggg gcggtttcct gaatcagacg gccggattca tcatatttgc
     3781 ggcgcatttc cagcccgtct ggcccggtgg ctgacgttaa ctggctgtgg tggttatagt
     3841 aaaacgccga tgccctgcca tccggcgtgg tgatgcgcgt gatgaggccc gtcaccacat
     3901 ccgggctgta ctctgttgtc cggcctgcgg catccgtctg tgtcctgagc ctgcccacgg
     3961 cgtcaaactg actctgcgtg acgctgccgt ccgcgtgttc ctttttcacc acccgcttca
     4021 gcccggcttc gccctgcgtg tgcagcactt cacggcggtc caggctgtcg gtgatggtga
     4081 tgcggtcctt ctcatactga tacgtgtaac ttaagcctgc cgggtttagc tgttctgtca
     4141 cccgcccgtc gctgtcgtaa cggtaacgga tttccggccg gcccgtgtga cggtgcgcca
     4201 ccatccggcc ccggtattta tcatcgtaag taaagctgcg cacctgtttg ccactacggt
     4261 catacaccac cgccagttcg ccgcgcggcg tccagccata gcgtaccagc ggcgcggcag
     4321 gtaaattctc cgggtattcc gggtcgtgcg tcagccacac ggcagacaga cggatgccgt
     4381 tgtcccggcc atattcggtg taacccggca gggtatcagg aaaagcggac ggttccgtcc
     4441 cgccggaaat ggcctgctgc cgggcttctt ctgcccgctg cgcctgcgtg gtcagtacca
     4501 gccggaagtg acgcccggca ccatccgtca cgccggtgat ttcgccgctg aattcaccgg
     4561 cggcttcgcg gtggaacgtc tgtgtgcgcc cgaagcggtc caccagcccg gtcagtaccc
     4621 ggtacggcgg cagcggcgca ggcagcacct catccgcttc cggcacccgc tcacaccagc
     4681 cgagcagcca ccacggcccc tgcggactgt ttgtcgccag ataacgatgc ggacttaagc
     4741 ggagttcttc cggcagcgcc tgccagagtg cggccagccg gtgaccttca tccagtttcg
     4801 ccacgccgcc gcgcaccagc cacagtgact cgctgcggct gtaaccgtcc tcaccgggaa
     4861 acaggtgctc aaaatacagg cttctgccgc cgttatcact gagtatcagt gtgttatcgc
     4921 gcagctgtaa gcggatatcc gcaggcattt tccagccggg gccgaggctc cccaccggcg
     4981 cgggcgtttt tgtccggtaa ctgctgtagg tgcgggagag gatgaacggc agcgggccgg
     5041 gcagggcgat gtcggtttca ccgggaagga cctttgcacc gagcagggga ttgaccggat
     5101 ggccggacgt caccccgccg gggcacaccg aacaggccac gccgatgggg gcaccgatgc
     5161 gcaccccggc tgaaccctgg acaatgctac cgccatactg cgtcatgtcg ccctgacgcg
     5221 ccgccggttt tccgctcatc gtaaaatcct tttgaaataa ataagaacca gaagcctagc
     5281 gccagtcaac aacaggaaat gataatgagt taacaccccg gaatttttca gaatttataa
     5341 aaggcaaatt acaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt
     5401 atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa
     5461 catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg aggttcaggt
     5521 catgaccaac ggtggagcgg attttcatta cgttaccgca agagcagcta gcagtaattt
     5581 cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc
     5641 tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca atacgatcat
     5701 ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact gatccgcact
     5761 cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat gctatgcccg
     5821 ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg ctgccagaag
     5881 gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca
     5941 tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcaatgag ctaaaagcgg
     6001 tagtcgaagt gctggatagt gagccggtgt ttactcactc cgtctggcga ttgctgctat
     6061 gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc ttgccgattt
     6121 tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt gccactgaac
     6181 aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa caggcgctgg
     6241 cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa tttaatgatg
     6301 ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt
     6361 ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg aataccgaac
     6421 aggccaccgc cgttggcgca attcatagtg cggcggatac cttctctgcc tggctgctgg
     6481 cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa aacgtgctcg
     6541 ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg caaactatcg
     6601 cccgttttcg tgaacgtttt aatgcccccg tggaagttct gcattccggc ctgaacgaca
     6661 gcgagcgtct ttcggcgtgg ttgaaagcga aaaatggcga ggcggcgatt gtcatcggca
     6721 cccgctccgc gctgtttacg ccgtttaaaa atctcggggt gattgtcatt gatgaagagc
     6781 acgacagctc ctacaagcag caggaaggct ggcgctatca tgcccgcgac ctggcggttt
     6841 atcgtgcgca cagcgagcaa atcccgatta ttcttggctc tgcaacaccc gcactggaaa
     6901 cgttatgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgt cgggcgggga
     6961 atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg caggcggggc
     7021 tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccgacaac caggttatcc
     7081 tctttcttaa ccgccgtggc tttgcgcctg ccttgctgtg ccacgactgc ggctggattg
     7141 ccgaatgtcc acgttgcgat cactactaca cgctgcatca ggcgcagcaa catctgcgct
     7201 gccaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc
     7261 acctggtacc cgtagggctg ggcaccgaac agcttgagca aacgctcgcg ccgctgttcc
     7321 ctggtgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg gcgctggaac
     7381 agcaactggc ggaagtgcat cgcggcggcg cgaggatctt gattggcacg caaatgctgg
     7441 cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc
     7501 tgttttctgc cgattttcgc tcggcagaac gtttcgccca gctttacacc caggtcgccg
     7561 gtcgtgccgg gcgtgcgggt aaacagggcg aagtggtgct gcaaacgcac catccggaac
     7621 atcctctgtt gcaaacgttg ctctataaag gctacgacgc ctttgccgaa caggcgctgg
     7681 ctgagcgacg catgatgcag ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag
     7741 atcataacaa tcagcacgcg ccgctgttcc tgcaacaact gcgtaatctg atcctttcca
     7801 gcccgctggc agacgacaaa ctgtgggttc tcggcccggt tccggctctg gcacctaagc
     7861 gtggtggccg ctggcgctgg cagatcttgt tgcagcaccc ttcccgcgtg cgcttgcaac
     7921 acatcattag tggtacgctg gcgcttatta atacaatacc ggattcccgc aaggtgaaat
     7981 gggtgttgga tgtcgatccc atcgaaggct aatgcgcaaa cgttgcgagg cggatcgaaa
     8041 aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg cgctatctgc
     8101 taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg
     8161 agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa
     8221 ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata aagtctccca
     8281 ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac cgcagcctat
     8341 ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc cggatatctg
     8401 cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa atcacggata
     8461 tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt
     8521 gatcatcacc aagcaaattg atggcatgtt gttgctgggt tcaaggctgc cgtttgatgc
     8581 cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat ttgcaccgga
     8641 gctggagctg cctacagttc atatcgacaa tctgaccgcc gcatttgatg cagtaaatta
     8701 tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag agatgccgct
     8761 gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggca ttatggttga
     8821 tccgcaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag cgatgcagca
     8881 gctgcttgat cttccacaac cgcctactgc tgtcttctgc catagcgatg tgatggcgct
     8941 cggcgcactt tctcaggcaa aacaccaggg gctgaaagtc ccggaagacc tttccataat
     9001 cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca
     9061 gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa tgcaggggca
     9121 acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg gatcaacacg
     9181 cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa
     9241 gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca caacgagatt
     9301 atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc
     9361 aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc gttcttgtga
     9421 cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc gagacgctgc
     9481 aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc tggcgctaca
     9541 ttaaagagct ggaaagtcgc cagccgggag tgcgtgcgcc cacagaacct tctgccggtg
     9601 gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt gaacaaatgc
     9661 aggctgatat gcgccagcag ccaacgcagc tggttgaagt tccgtggaat gaacagacgc
     9721 cggaacagcg tcagcaaacg ctacagcgcc aacgtcaggc gcagcaatta gcggaacagc
     9781 aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag acgcgtacgt
     9841 cgcaagccgc accggtacag gcgcaaccgc gccagtctaa accggcttct tctcagcagc
     9901 cgtaccagga tctgctgcaa actcctgcgc acacgactgc gcagtcgaag ccgcagcaag
     9961 ctgcgccagt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac
    10021 gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag acggtacgtg
    10081 ctcagctggc gttcgaaggc tttgactcga aaatcaccac caacaatggc tggaatcgtg
    10141 tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat cggttgaaga
    10201 tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct caaaatcccc
    10261 cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc tgtattcgta
    10321 accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc atgtggtcat
    10381 cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt
    10441 ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg cggatgcttt
    10501 tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc tggtcaaagc
    10561 cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact
    10621 gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca
    10681 gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg ctgcggcgcg
    10741 cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg cgttggatat
    10801 tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat taagctacaa
    10861 agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg aactggataa
    10921 gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc gtaaccgctg
    10981 gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa atatcctgat
    11041 gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta agctggcgaa
    11101 tgcgccgttc atcaaagttg aagcgaccaa attcaccgaa gtgggctacg tcggtaagga
    11161 agtggattct attattcgcg atctgaccga tgccgccgtg aaaatggtac gcgtccaggc
    11221 tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc tcgacgtgct
    11281 gatcccacct gctaaaaaca actggggaca gaccgaacag cagcaggaac cgtccgctgc
    11341 tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag aaatcgagat
    11401 cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca tggaagagat
    11461 gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa
    11521 gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga aactggtgaa
    11581 cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga tcgtgtttat
    11641 cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga
    11701 aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca ccaaacacgg
    11761 gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc
    11821 gtctgacctg atcccggaac tgcaaggtcg tctgccaatc cgcgttgaac tgcaggcgct
    11881 gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca ccgtgcagta
    11941 caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg gtattaaacg
    12001 catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt
    12061 acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg atttaagcgg
    12121 tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt tggtggcaga
    12181 tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca ttgtttgatg
    12241 gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa ttagccgaac
    12301 tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg ctgcaattat
    12361 cgtcgggaca gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg ccctgctggc
    12421 actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt acggcgatgc
    12481 ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt
    12541 cattacccag caagagatga aacgggcgct cattattacc gtcgtgctca tctgtctctc
    12601 cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg gtttcctgat
    12661 tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc gtccttatgg
    12721 ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga gtgtcatggg
    12781 gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg cgaccgcatg
    12841 cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca atagcgaccg
    12901 cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca
    12961 tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct tttcgctgca
    13021 tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc aagcccgtta
    13081 tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta ctgtcaaggg
    13141 agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt gggcagcata
    13201 actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag cgcgatatac
    13261 tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc cgagctttgt
    13321 gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt tggcggacgg
    13381 gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg gttgctgtac
    13441 gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt
    13501 cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga atgggaaggt
    13561 ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga tatcggcatc
    13621 caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga aagcgatgtc
    13681 cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc cgacaatacc
    13741 gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca ccgcaaggtg
    13801 ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgga agatgaagcg
    13861 taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc agccgttctg
    13921 ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct gggcattttg
    13981 aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca tctgcaacag
    14041 agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca cttctaatga
    14101 cattgtcata cctctcctga attgcaaggc gttgatggat aaaaatcctc gtcccgatta
    14161 ccggtgacgc cttaataaat acgagcgcac tttagttagc tccgattgta tgaagccgcg
    14221 ccatcgctgt ccagcggcac gccttgcaga ttacggtttg ccacactttt catccttctc
    14281 ctggtgacat aatccacatc aatcgaaaat gttaataaat ttgttgcgcg aatgatctaa
    14341 caaacatgca tcatgtacaa tcagatggaa taaatggcgc gataacgctc attttatgac
    14401 gaggcacaca cattttaagt tcgatatttc tcgtttttgc tcgttaacga taagtttaca
    14461 gcatgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa cattaactct
    14521 tcaggatccg attatgagtc aaacatcaac cttgaaaggc cagtgcattg ctgaattcct
    14581 cggtaccggg ttgttgattt tcttcggtgt gggttgcgtt gcagcactaa aagtcgctgg
    14641 tgcgtctttt ggtcagtggg aaatcagtgt catttgggga ctgggggtgg caatggccat
    14701 ctacctgacc gcaggggttt ccggcgcgca tcttaatccc gctgttacca ttgcattgtg
    14761 gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt atcgtttcac aagttgccgg
    14821 cgctttctgt gctgcggctt tagtttacgg gctttactac aatttatttt tcgacttcga
    14881 gcagactcat cacattgttc gcggcagcgt tgaaagtgtt gatctggctg gcactttctc
    14941 tacttaccct aatcctcata tcaattttgt gcaggctttc gcagttgaga tggtgattac
    15001 cgctattctg atggggctga tcctggcgtt aacggacgat ggcaacggtg taccacgcgg
    15061 ccctttggct cccttgctga ttggtctact gattgcggtc attggcgcat ctatgggccc
    15121 attgacaggt tttgccatga acccagcgcg tgacttcggt ccgaaagtct ttgcctggct
    15181 ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac attccttact tcctggtgcc
    15241 gcttttcggc cctatcgttg gcgcgattgt aggtgcattt gcctaccgca aactgattgg
    15301 tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag gaaaccacaa ctccttcaga
    15361 acaaaaagct tcgctgtaat atgactacgg gacaattaaa catgactgaa aaaaaatata
    15421 tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat cacgatgcca
    15481 atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca ggttgggtag
    15541 aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa gtgctggcga
    15601 aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag cgtgaaacca
    15661 ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc tggcagtgcc
    15721 gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat tatatccgca
    15781 gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag tggatcctcg
    15841 accatgtgga aggctctcgc gagcgtgcac gtcgtggtga attgctgttt ggtacggttg
    15901 atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat tacaccaacg
    15961 cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa atgctggaag
    16021 tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa gtatacggtc
    16081 agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc gccggtgacc
    16141 agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag aacacctatg
    16201 gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca gaaaacggcc
    16261 tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg gaaggtgcgg
    16321 tgtttatggc aggcgcatcc attcagtggc tgcgcgatga aatgaagttg attaacgacg
    16381 cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg tatgtggttc
    16441 cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg gcgattttcg
    16501 gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag tctattgctt
    16561 atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt ctgcacgccc
    16621 tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag tccgatattc
    16681 tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt gcggcctatc
    16741 tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag aaagcggtga
    16801 ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt tacgcaggct
    16861 ggaaaaaagc ggttaaacgc gcgatggcgt gggaagaaca cgacgaataa tgtaaatgcc
    16921 gaatgaagcg tttatgccgc atccggtagt cccgaaacgt gcgggggcaa ccccgcacac
    16981 atcaataatc cctcccttcc cctgtgctac acttcgcgcc attccttact gcttagagtt
    17041 tgctatgaga cgagaacttg ccatcgaatt ttcccgcgtc accgaatcag cggcgctggc
    17101 tggctacaaa tggttaggac gcggcgataa aaacaccgcg gacggcgcgg cggtaaacgc
    17161 catgcgtatt atgctcaacc aggtcaacat tgacggcacc atcgtcattg gtgaaggtga
    17221 aatcgacgaa gcaccgatgc tctacattgg tgaaaaagtc ggtactggtc gcggcgacgc
    17281 ggtagatatt gctgttgatc cgattgaagg cacgcgcatg acggcgatgg gccaggctaa
    17341 cgcgctggcg gtgctggcag taggcgataa aggctgcttc ctcaatgcgc cggatatgta
    17401 tatggagaag ctgattgtcg ggccgggagc caaaggcacc attgatctga acctgccgct
    17461 ggcggataac ctgcgcaatg tagcggcggc gctcggcaaa ccgttgagcg aactgacggt
    17521 aacgattctg gctaaaccac gccacgatgc cgttatcgct gaaatgcagc aactcggcgt
    17581 acgcgtattt gctattccgg acggcgacgt tgcggcctca attctcacct gtatgccaga
    17641 cagcgaagtt gacgtgctgt acggtattgg tggcgcgccg gaaggcgtag tttctgcggc
    17701 ggtgatccgc gcattagatg gcgacatgaa cggtcgtctg ctggcgcgtc atgacgtcaa
    17761 aggcgacaac gaagagaatc gtcgcattgg cgagcaggag ctggcacgct gcaaagcgat
    17821 gggcatcgaa gccggtaaag tattgcgcct gggcgatatg gcgcgcagcg ataacgtcat
    17881 cttctctgcc accggtatta ccaaaggcga tctgctggaa ggcattagcc gcaaaggcaa
    17941 tatcgcgact accgaaacgc tgctgatccg cggcaagtca cgtaccattc gccgcattca
    18001 gtccatccac tatctggatc gcaaagaccc ggaaatgcag gtgcacatcc tctgattgat
    18061 ttgatcgatt gagccttcca gtccttcggg actggaattt ttttgttcgg agaacgaaga
    18121 taaggcaagt caatcaaaac aggagaaaaa catggctgat tgggtaacag gcaaagtcac
    18181 taaagtgcag aactggaccg acgccctgtt tagtctcacc gttcacgccc ccgtgcttcc
    18241 gtttaccgcc gggcaattta ccaagcttgg ccttgaaatc gacggcgaac gcgtccagcg
    18301 cgcctactcc tatgtaaact cgcccgataa tcccgatctg gagttttacc tggtcaccgt
    18361 ccccgatggc aaattaagcc cacgactggc ggcactgaaa ccaggcgatg aagtgcaggt
    18421 ggttagcgaa gcggcaggat tctttgtgct cgatgaagtg ccgcactgcg aaacgctatg
    18481 gatgctggca accggtacag cgattggccc ttatttatcg attctgcaac taggtaaaga
    18541 tttagatcgc ttcaaaaatc tggtcctggt gcacgccgca cgttatgccg ccgacttaag
    18601 ctatttgcca ctgatgcagg aactggaaaa acgctacgaa ggaaaactgc gcattcagac
    18661 ggtggtcagt cgggaaacgg cagcggggtc gctcaccgga cggataccgg cattaattga
    18721 aagtggggaa ctggaaagca cgattggcct gccgatgaat aaagaaacca gccatgtgat
    18781 gctgtgcggc aatccacaga tggtgcgcga tacacaacag ttgctgaaag agacccggca
    18841 gatgacgaaa catttacgtc gccgaccggg ccatatgaca gcggagcatt actggtaagc
    18901 ggttacttat cgataaacgg cacgatgagc aaatccgcac tcatcttatt gatcatcccg
    18961 cgatatgccg gcatcaaacg gttgataaat gagtgatgat gaccacagac aaggaggtcg
    19021 cactgctctt tttgcataat ttccagcagt gtttccggca tttctccgcg ttcaatacgc
    19081 agttttgtct tcggccattg aatatttttc gtcagtttat acagcttgtt atccgactta
    19141 ttcttcaaca attgaagaat atcttctgtt gcagggaagt agatacccgg atacaactcg
    19201 cttaagccat catcaatatg aattaacgtc aggtgagcgt cattatgtct ggcgagctcc
    19261 agggctttat tcaccagtaa ggcatcttct tcattcccgg aaattgccac gccaatgtgt
    19321 ttataagcca tgtttaactc cttctaaagc aactccatca agctaacgaa cgcagtgata
    19381 actcaaaaat aatcatctcg gcctggcatg agaaagtgaa ggccgcatca agctcaactt
    19441 gcccctccag atccttaatc ttataggcca cgctggcccc ttcatctgca gaggatgcag
    19501 cggcggcaag ctctttcagt ttggtcataa aagactctgc ttcttcgcgg gtggcaaaaa
    19561 agcgcgaaaa cttactggta cagttgttgt tatcaatcac cgtaccgata tcaatcgcac
    19621 atcctttagt actgcattta tctacgacat ctttcatagg gcacctctgt atattcgcct
    19681 ctctgtcgat tacgacttaa gaataatgcg ccgttaaggg aagaacaaac aagtaatgat
    19741 cttatggatt accatttttt ttgatattgc ttaaggtgaa gagttcagaa ggattttccg
    19801 ggcattgtca ttttcatcgt ggaaataaca atcccggaaa aaaatagcag taagagatat
    19861 attaaaggtt aattacttga tttattgtcg gctttatact tcacatcctg ggtatcttta
    19921 ccatatttat tttcgccttg tgtgccaaca aacgcaccaa gatcgataag catcatcacc
    19981 agaatcaacg tcgggacaaa acgccccacc gcccattgcc agacacccgg taaaatcgcc
    20041 cagttacccg ccagcagcat ccacgccaca atcatcagaa atgcccatgc gccggaacgc
    20101 ccgcgatcat gcaagcgctt aacggttact gccgctgttg gccagagcaa gcacacaagg
    20161 caaaacgccg cggtctgaat atcgagtaaa tttttacccg ccagtgagaa aagcaccagc
    20221 atgcctgcga accacaggcc tatccaaatc cagaaatcac ggcgtccaat acgcccttta
    20281 aatgagaata accattgctg tatggtcatg taagttcctt gatggttgtc ttttccagga
    20341 ttctaccctt ttgacaaggg cgacagatat cgttttaatc ggagccagtc ataacaaaag
    20401 gtactgtcaa tgaagccagg gtgtacgctg ttttttctct tatgttctgc attaaccgtt
    20461 acaacagcgg cgcatgcaca aacaccagat acggcaacga ccgcgcctta tctgctggct
    20521 ggagccccta ctttcgatct ctccatcagc cagtttcgag aagactttaa cagccagaat
    20581 cccagcctgc cactgaacga atttcgtgcc atcgacagca gtcccgacaa agccaatctc
    20641 actcgtgctg caagtaaaat taatgagaac ttgtatgctt ctacagcgct ggagcgcggt
    20701 accttaaaaa tcaaaagcat tcaaatgacc tggctaccca tccaggggcc agagcaaaaa
    20761 gccgcgaaag cgaaagctca ggaatacatg gcagcggtga tccgcacact caccccatta
    20821 atgaccaaaa cacaaagcca gaaaaaactg cagtcgctac taacggcggg gaaaaacaaa
    20881 cgttattaca ccgagacaga aggtgcactg cgttatgttg tcgcggacaa cggcgaaaag
    20941 gggctgacct tcgctgttga accgattaag ctggcgctat ctgaatcgct tgaaggtttg
    21001 aataaatgac aaaaagcaaa gcctttgtgc cgatgaatct ctatactgtt tcacagacct
    21061 gctgccctgc ggggcggcca tcttccttta ttcgcttata agcgtggaga attaaaatgc
    21121 gacatccttt agtgatgggt aactggaaac tgaacggcag ccgccacatg gttcacgagc
    21181 tggtttctaa cctgcgtaaa gagctggcag gtgttgctgg ctgtgcggtt gcaatcgcac
    21241 caccggaaat gtacatcgat atggcgaagc gcgaagctga aggcagccac atcatgctgg
    21301 gtgcgcaaaa cgtggacctg aacctgtccg gcgcattcac cggtgaaacc tctgctgcta
    21361 tgctgaaaga catcggcgca cagtacatca tcatcggtca ctctgaacgt cgtacttacc
    21421 acaaagaatc tgacgaactg atcgcgaaaa aattcgcggt gctgaaagag cagggcctga
    21481 ctccggttct gtgcatcggt gaaaccgaag ctgaaaacga agcgggcaaa actgaagaag
    21541 tttgcgcacg tcagatcgac gcggtactga aaactcaggg tgctgcggca ttcgaaggtg
    21601 cggttatcgc ttacgaaccc gtatgggcaa tcggtactgg caaatctgca actccggctc
    21661 aggcacaggc tgttcacaaa ttcatccgtg accacatcgc taaagttgac gctaacatcg
    21721 ctgaacaagt gatcattcag tacggcggct ctgtaaacgc gtctaacgct gcagaactgt
    21781 ttgctcagcc agacatcgac ggcgcgctgg ttggcggtgc gtctctgaaa gctgacgctt
    21841 tcgcagtaat cgttaaagct gcagaagcgg ctaaacaggc ttaagtctga caggtgccgg
    21901 atttcatatc cggcacttac tttccttaac tcttcgcctt aacgcaaaat ctcacactga
    21961 tgatcctgaa tttcctcggc tgaagcacgg ttaagcgtca gtagatttcg ttgtgtcgcc
    22021 agcaatacaa atgagttatc actctgccgt accatcgcca gcccgtagct tcccatatgt
    22081 tcccgcgcct caggcacttc ttccgccagc atcataaatg ggctgcgttg taccagttcg
    22141 ctttccgtta cccgacgcgc caagtattca tgcccgcgca aaccacctgg cagtggcaac
    22201 cagcggctgc tgatgttcgc cagattgtta tccagctgtt cgcgcacatc aggacgaata
    22261 caagagatat gaatatgaaa atggttttgc gtacgcccgg tgcgggagtt gatcgccaaa
    22321 gaaaccgcgc gatcgggaac cggctggccg tattttttgc tcataaaatc acgcgcctgc
    22381 caagccaacc aaaagaagtt cggcgttgaa ggatcggtca acaaaggact ttcagtaccg
    22441 ttaatacgat acgttggcat caacagatat tgcagtgggc cattaagatc ttttaaaacc
    22501 acgtatccgg cattgggttt gacttccgca catggcgaag gattttgatt ttgctgctga
    22561 ttgggcaaac attcctcaag gacaatctta cgtaatgtat ccgactcttc accggttaat
    22621 ttccagtaac caataccggc agccacaacg gcgataacta tcatcaccaa aaaaagaaga
    22681 cccgcttttt tcatcttttt tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg
    22741 tgacaaataa aaaacccggt cgcgagaaac caccgggttt gataattatc ctggggaaat
    22801 cagcgtttgc tgatctgatc gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc
    22861 gtccagccgc cgaactcttc atcaatggtg aataacttca gctttggaaa cgcattttcg
    22921 tactttttcg ccacctcagc gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga
    22981 ccttctggcg agtagagata tttcaggtag gcttccgcca cctctttagt accttttttc
    23041 tcgaccactt tatcgaccac cgacacggtt ggctctgcga ggatagactc actcggcgtg
    23101 acgatttcga atttatcttt ccccagttca ttcgctgcca gcagagcttc gttttcccag
    23161 gcaatcagta catcgccaat tccgcgctcg acaaaagtgt tagtggagcc acgcgcgcca
    23221 gaatccagaa cttcgacgtt tttatacagt gcccgaacaa aatcctgtgc ttttgcctga
    23281 tcgttgttgt tgtgatgcag cgcgtagccc caggctgcca ggtagttcca gcgcgcgcca
    23341 ccagagcttt tcggattagg cgtgatcacc gaaacacccg gtttaatcag atcgttccag
    23401 tcatggatct gcttcggatt tcccttacgt accaggaaaa caatggtgga agtgtacggt
    23461 gcggagttat ccggcagacg tttgatccac tctttatcaa tccgcccgcg ttccgcaatt
    23521 gcgtccacgt cataggccag agccagcgtg acaacatcag cttcaatacc gttgattacc
    23581 gacgtcgctt gtttacctga gccaccgtgt gactgacgaa tcaccacgtt atcaccagtt
    23641 tgctgtttcc agtgggcgct gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga
    23701 tcatatgaaa cgttaagaag ctgaatatcc tttgccataa cgctggttgc cgccagcaaa
    23761 aatgttaacc ctacgcccca cttgttcatc gcccgactct cttatgttgt gttgtgatga
    23821 gcaaagcgtg ccagaaggtt aaccagacat taaagaataa aaaaagattg gctataactt
    23881 gcgggtatat gttgggggat taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag
    23941 tgcgctttgt ccatgccgga tgcggcgcga acgccttatc cggcctacaa aagtttgcaa
    24001 attcaataga ttacagaatt catgtagacc tgataagcga agcgcatcag gcctttttgc
    24061 ttctgtcatc ggtttcaggc taaaggaatc tgcctttttc cgaaatcatt aatacagttt
    24121 tttcgcgcag tccagccagt cgcctttgaa cggacgcttc atgttttcga tagcgtcgat
    24181 gatgtcgtgg tgaaccaact gttcgttctg gatacctacg caacgaccgc cgtaacctgc
    24241 cagcagcaga tcgatagcgt aagcgcccat acgggaagcc agaatacggt cgtaaggcac
    24301 cggagaacca ccgcgctgga tgtggcccag cacagttgcg cgggtttcac gaccggtttc
    24361 tttctcgatg aaatgcgcca gttcgtcaac atcacacata tgttcggtaa tcgccacgat
    24421 cgcgtgtttt ttacctttcg cgatacccgc tttgatttca tttaccaggt cttcacggct
    24481 gaattcaact tccggaacca caacgaattc acagccaccg gcaatggccg cagccagcgt
    24541 cagatcgcca caataacggc ccatcacttc caccacggaa atacgctggt gagaagaaga
    24601 ggtgtcacgc agacggtcga tcgcttctac aacggtgctc agcgcagtga agaaaccgat
    24661 agtgtagtca gtgcctttga tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa
    24721 gcccatttcg gtcagacgca ttgcacccat gtaggaaccg tcaccgccga taaccaccag
    24781 cgcgtcgata ccacgttttt tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg
    24841 gaattccggg aagcgcgcag aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac
    24901 gctgtaacgg tctagctgta ccatacggtc ttcatacaga cccagatagc cgtcataaat
    24961 gcccattact tccagacctt ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt
    25021 catgcctggc gcatcaccgc cgcttgtcaa cacaccgatt ttcttaatca tgactacctc
    25081 tgaactttgg aatgcaaaat aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat
    25141 ccaacgaatg tgcaaatagt ataacaatca cttcctgctg aattgattca ggtcaggcca
    25201 aatggcggta ttttatacac aaaatgcggg tctggctctc ttttatactg attatgaaag
    25261 catagaccgt ttaccctccc tgggtacgac ggaacagggg tcctgatgga taattacatc
    25321 cgatcccgga aaacgccgta aaatagcctg ctctacctga tccgccacca tatgtgcctg
    25381 aaccaaaggc agagagtctt ccatttccaa atgaatctga ataaagcggg tcggccctga
    25441 ctgccgcgtg cgaagatcgt gagcgccgct aacacccggc caggaagtca cgatatcaat
    25501 aatttcttgc cgttcctcat caggcaatgc gcgatccagt aatgactgta ccgcctcata
    25561 tcccatgcgt aacgcgctat ataaaatata gatgccgatt cccaatgcaa acagagcatc
    25621 ggcgcgatgc cagccgtacc aggacaaccc cagcgccagc agaattgcgc cgttcatcat
    25681 aacatcagac tggtaatgta gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac
    25741 cacccaacgc tgaaacgaga caaggataat cgtacaaatt agcgccacaa ttgtcacgat
    25801 aaccccgacg cctggatctg tcatcggtgt tggagatatc agatgttgaa tacccgtcaa
    25861 aaacaggaat agtgccgaac cggagataaa catactttgc gccagcgccg cgagggactc
    25921 tgctttaccg tgaccaaacg agtgattatc gtcggcaggt tgcagggaat atcgcaccac
    25981 cagtaaattc gtcaacgacg cgccgatatc caccagcgaa tccaccagcg cggcgagaat
    26041 actcaccgac ccggtatacc accatgcaaa aattttaatc agcaatagca acgaagccat
    26101 cgccgtcgca gcaatcgccg cccgactgac cagccgtcca taagattgat tcataaatac
    26161 tcccgctatc aactgacgct agtataacgg aagcaaatca tctgcaatgc attaagcagc
    26221 aggcaaattg aggataaaaa aaacccccac atcatgtggg ggaagacagg gatggtgtct
    26281 atggcaagga aaacagggtt tactactggg aacgtgagtt gctactactc aatagcttca
    26341 acgatgaact tttttgccat tgcgtcacgt cacgcaactg ctccattcgt tgttgatgtt
    26401 tctcgtttaa aaccgcttgc tgctccggcg ttaacaggcg atacatttgg ttgcggactt
    26461 ttgccatctc aacctgacga gcaatttgct cattcgccat tttttctgcc tgtgcgcgca
    26521 cagcgttttc atcaaaattt tctgcggtga caaggcgatg cattgtctcc agttcgctaa
    26581 cattaacagg aggctgttcg tgccgggcct gttgcataag atctcgcatc tgctgacgct
    26641 gatgttcggt taaacttatg ccgtcgaaca tatggctctg cgtactgcgc tgcgtaagtt
    26701 cttcacccgg atgccagtta tcgcctgaac cgacttcagc agcgtggctt aatgaactga
    26761 ctgccagcgt tgaggccatg acggcagcgg taactatgcg catcatttgc tcccaaaatc
    26821 tttctgtcgc gattcaacga tagagagttt acgattcagg ctgcaaacat gcgtcagggg
    26881 gtgtaaaaca acgtaaagtc atggattagc gacgtctgat gacgtaattt ctgcctcgga
    26941 ggtatttaaa caatgaataa aatcctgtta gttgatgatg accgagagct gacttcccta
    27001 ttaaaggagc tgctcgagat ggaaggcttc aacgtgattg ttgcccacga tggggaacag
    27061 gcgcttgatc ttctggacga cagcattgat ttacttttgc ttgacgtaat gatgccgaag
    27121 aaaaatggta tcgacacatt aaaagcactt cgccagacac accagacgcc tgtcattatg
    27181 ttgacggcgc gcggcagtga acttgatcgc gttctcggcc ttgagctggg cgcagatgac
    27241 tatctcccga aaccgtttaa tgatcgtgag ctggtggcac gtattcgcgc gatcctgcgc
    27301 cgttcgcact ggagcgagca acagcaaaac aacgacaacg gttcaccgac actggaagtt
    27361 gatgctttag tgctgaatcc aggccgtcag gaagccagct tcgacgggca aacgctggag
    27421 ttaaccggca ctgagtttac cctgctctat ttgctggcac agcatctggg tcaggtggtt
    27481 tcccgtgaac atttaagcca ggaagtgctg ggcaaacgcc tgacgccttt cgaccgcgct
    27541 atcgatatgc acatttccaa cctgcgtcgt aaactgccgg atcgtaaaga tggtcacccg
    27601 tggtttaaaa ccttgcgtgg tcgcggctat ctgatggttt ctgcttcatg ataggcagct
    27661 taaccgcgcg catcttcgcc atcttctggc tgacgctggc gctggtgttg atgttggttt
    27721 tgatgttacc caagctcgat tcacgccaga tgaccgagct tctggatagc gaacagcgtc
    27781 aggggctgat gattgagcag catgtcgaag cggagctggc gaacgatccg cccaacgatt
    27841 taatgtggtg gcggcgtctg ttccgggcga ttgataagtg ggcaccgcca ggacagcgtt
    27901 tgttattggt gaccaccgaa ggccgcgtga tcggcgctga acgcagcgaa atgcagatca
    27961 ttcgtaactt tattggtcag gccgataacg ccgatcatcc gcagaagaaa aagtatggcc
    28021 gcgtggaact ggtcggtccg ttctccgtgc gtgatggcga agataattac caactttatc
    28081 tgattcgtcc ggccagcagt tctcaatccg atttcattaa cttactgttt gaccgcccgc
    28141 tattactgct gattgtcacc atgttggtca gtacgccgct gctgttgtgg ttggcctgga
    28201 gtctggcaaa accggcgcgt aagctgaaaa acgctgccga tgaagttgcc cagggaaact
    28261 tacgccagca cccggaactg gaagcggggc cacaggaatt ccttgccgca ggtgccagtt
    28321 ttaaccagat ggtcaccgcg ctggggcgca tgatgacctc tcagcagcgt ctgctttctg
    28381 atatctctca cgagctgcgc accccgctga cgcgtctgca actgggtacg gcgttactgc
    28441 gccgtcgtag cggtgaaagc aaggaactgg agcgtattga aaccgaagcg caacgtctgg
    28501 acagcatgat caacgatctg ttggtgatgt cacgtaatca gcaaaaaaac gcgctggtta
    28561 gcgaaaccat caaagccaac cagttgtgga gtgaagtgct ggataacgcg gcgttcgaag
    28621 ccgagcaaat gggcaagtcg ttgacagtta acttcccgcc tgggccgtgg ccgctgtacg
    28681 gcaatccgaa cgccctggaa agtgcgctgg aaaacattgt tcgtaatgct ctgcgttatt
    28741 cccatacgaa gattgaagtg ggctttgcgg tagataaaga cggtatcacc attacggtgg
    28801 acgacgatgg tcctggcgtt agcccggaag atcgcgaaca gattttccgt ccgttctatc
    28861 ggaccgatga agcacgcgat cgtgaatctg gcggtacagg attggggctg gcgattgttg
    28921 aaaccgccat tcagcagcat cgtggctggg tgaaggcaga agacagcccg ctgggcggtt
    28981 tacggctggt gatttggttg ccgctgtata agcggagtta aactccgcat ttgtaggcag
    29041 gataaggcgt ttacgccgca tccggcattt gagcaggatg cctgatgcga cgctgatagc
    29101 gtcttatcag gcctacactc cgggggttat tttccccaca accggcggga aaaatcttcg
    29161 atcttgccgc tcagtcggcg cttttgcatc gtccgcgtcc agcttttcga taacccggcg
    29221 gcggagagta aacggtgata ttgatcgtca tcaaacggca tatgccatgc gatggcggca
    29281 gcctcctgca cggtcacatc gctgacacgg gaaaccaact ccagcggtgc gtccgcagac
    29341 actttccccg gcgctatcac gctatacaac caccccactt tacccgtgtt ttgcatcaac
    29401 tgcgctatgt cgctgatatc aaaatggtaa ttgagtttat agcaaggcga gcgcggctgg
    29461 ctgacctgaa ttaatgcctc tccccagcgg aaaatatcgc ccatataaac attactttcc
    29521 gtgagaccgt cggttgagag attttcgcca aacgcaggcg caacaaacaa ctccgcctgt
    29581 tccggaaatt cccgcgccca gtagagatag tgctcacgag gataatgaca cagcgctctg
    29641 tctggcccgc cgtgaacctt tttctccgcc tgctcgtcac cttccagccc cagctccgtc
    29701 agcatcaact caccatcaac ctggattttg gcaattgcgc tgggtttgcc ttcgggataa
    29761 gcctgaatct tgcctgtgta tacatcaacc ggatatcgca tctgttccct ctccacgaga
    29821 aatttcccca ttagaccata tttattttat gtcaggcgtg ctgttttcgc ggtatgtatc
    29881 caggcaggag ctcaatagca aaccggacga acgttagttc atccggctgt ggggattaag
    29941 gaagcaggtt tattttaccg tgcctaaaat ttcgattttc gccactctgg ctttgacttt
    30001 acgtgaccag atagaggtaa gaattggcac cagaatcgaa gtcacaatga ccgctgtcgc
    30061 gaccagcgaa gttgctgccg gagccatcgg tttaaacgca ggcaccattt cagcaatcag
    30121 cacaggtgtc gctaccgcgg cccctgcgga actggaagcg gcaattccgg cagtgccatc
    30181 gccaccgcca atcaatttat cggcgataat caacggaata ccggtcacga taattactgc
    30241 cacacccaac aggatcccca gcaaacctgt ctgggcaatt acagtcaaat caatggtatt
    30301 gcctagtgcg aaggcaaaga atggaatcag cgtctgcacc gctttgctga aaaattctcg
    30361 taattcaggg tcaaggttcc caagggcaaa gcccaccagg aacggtaata ctgcgccgac
    30421 gaaaacatgc ggttcaaacg aggcaatccc ggcagtgccc agaataatca tcgtcatgag
    30481 cggcccggac tccaacgaca tcaacacaaa tgccccagct tcttcttttg tgccgtactg
    30541 ctgcatgatg gaagcgtaaa gtccgccgtt ggtcatatcc atcgccgcca ccagcgccag
    30601 cgttgaaagt ccggcaaaga atccaacttc aacaccatgt tccggaataa tacgcgaggc
    30661 aattgccgca accacccacg cgacggcaat tttagttacc accagcgtac cggatttacg
    30721 cagtaccgtt cccgtcgcgc ttaattttat tgacgccccc atgcaaaaaa accacaccgc
    30781 cagaatgggc accgtaccgg taatcatccc gttggtgaat gatccaaaat atttccccgc
    30841 ccccggcgag aaggtgtgcc acagtgcgcc aaggaatagc gggacgagca tcatcccccc
    30901 cgggattttc tcaatcgagc gttttatctg catttccatc acctgtcggc atatttttaa
    30961 gggctgatgc gtaattcctc aatggagcat cagtgttgat ggaaacgaag atagacattc
    31021 atttaatcaa taaaagtgat caaaaccaca tcaattgaaa cactgtttta tttttatcgg
    31081 atcattgttt ttattttgaa
//
LOCUS       DAOFQD010000056        30693 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.056, whole genome shotgun sequence.
ACCESSION   DAOFQD010000056 DAOFQD010000000
VERSION     DAOFQD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.056"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..842
                     /gene="ycjM"
                     /locus_tag="QBF46_002483"
     CDS             <1..842
                     /gene="ycjM"
                     /locus_tag="QBF46_002483"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDQ6674657.1"
                     /translation="AYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLP
                     SSNTTWFNFLASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYE
                     INVTYMDALSRRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLG
                     YNRAINRKKYHSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTIN
                     SSVMRIQRSNADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVM
                     WIK"
     gene            856..2148
                     /locus_tag="QBF46_002484"
     CDS             856..2148
                     /locus_tag="QBF46_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6674658.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPENVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            2169..3050
                     /locus_tag="QBF46_002485"
     CDS             2169..3050
                     /locus_tag="QBF46_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDQ6674659.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            3037..3879
                     /locus_tag="QBF46_002486"
     CDS             3037..3879
                     /locus_tag="QBF46_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDQ6674660.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLKRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            3910..4962
                     /locus_tag="QBF46_002487"
     CDS             3910..4962
                     /locus_tag="QBF46_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDQ6674661.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4980..5768
                     /locus_tag="QBF46_002488"
     CDS             4980..5768
                     /locus_tag="QBF46_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDQ6674662.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            5778..6833
                     /locus_tag="QBF46_002489"
                     /pseudo
     CDS             5778..6833
                     /locus_tag="QBF46_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            6830..9097
                     /gene="ycjT"
                     /locus_tag="QBF46_002490"
     CDS             6830..9097
                     /gene="ycjT"
                     /locus_tag="QBF46_002490"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDQ6674663.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADTSVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETLTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLCLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            9094..9753
                     /gene="pgmB"
                     /locus_tag="QBF46_002491"
     CDS             9094..9753
                     /gene="pgmB"
                     /locus_tag="QBF46_002491"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDQ6674664.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLM
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            9767..10849
                     /locus_tag="QBF46_002492"
     CDS             9767..10849
                     /locus_tag="QBF46_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6674665.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            10894..11799
                     /gene="ompG"
                     /locus_tag="QBF46_002493"
     CDS             10894..11799
                     /gene="ompG"
                     /locus_tag="QBF46_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDQ6674666.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11910..12908)
                     /locus_tag="QBF46_002494"
     CDS             complement(11910..12908)
                     /locus_tag="QBF46_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ6674667.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALKAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            13064..14461
                     /locus_tag="QBF46_002495"
     CDS             13064..14461
                     /locus_tag="QBF46_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDQ6674668.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGVRLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMSEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDVWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            14458..15519
                     /locus_tag="QBF46_002496"
     CDS             14458..15519
                     /locus_tag="QBF46_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDQ6674669.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIDQVKETLQKGKTPSEK"
     gene            15667..17208
                     /gene="tyrR"
                     /locus_tag="QBF46_002497"
     CDS             15667..17208
                     /gene="tyrR"
                     /locus_tag="QBF46_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDQ6674670.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(17252..17758)
                     /gene="tpx"
                     /locus_tag="QBF46_002498"
     CDS             complement(17252..17758)
                     /gene="tpx"
                     /locus_tag="QBF46_002498"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDQ6674671.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17877..18842
                     /gene="ycjG"
                     /locus_tag="QBF46_002499"
     CDS             17877..18842
                     /gene="ycjG"
                     /locus_tag="QBF46_002499"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDQ6674672.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEACVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(18817..19545)
                     /gene="mpaA"
                     /locus_tag="QBF46_002500"
     CDS             complement(18817..19545)
                     /gene="mpaA"
                     /locus_tag="QBF46_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDQ6674673.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIDDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(19836..20474)
                     /locus_tag="QBF46_002501"
     CDS             complement(19836..20474)
                     /locus_tag="QBF46_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6674674.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(20492..21424)
                     /locus_tag="QBF46_002502"
     CDS             complement(20492..21424)
                     /locus_tag="QBF46_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ6674675.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            complement(21424..22344)
                     /locus_tag="QBF46_002503"
     CDS             complement(21424..22344)
                     /locus_tag="QBF46_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ6674676.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            22482..23381
                     /gene="pgrR"
                     /locus_tag="QBF46_002504"
     CDS             22482..23381
                     /gene="pgrR"
                     /locus_tag="QBF46_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDQ6674677.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            23718..25331
                     /gene="mppA"
                     /locus_tag="QBF46_002505"
     CDS             23718..25331
                     /gene="mppA"
                     /locus_tag="QBF46_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDQ6674678.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(25382..26413)
                     /gene="ynaI"
                     /locus_tag="QBF46_002506"
     CDS             complement(25382..26413)
                     /gene="ynaI"
                     /locus_tag="QBF46_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDQ6674679.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            26657..26914
                     /locus_tag="QBF46_002507"
     CDS             26657..26914
                     /locus_tag="QBF46_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDQ6674680.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(26964..27914)
                     /gene="uspE"
                     /locus_tag="QBF46_002508"
     CDS             complement(26964..27914)
                     /gene="uspE"
                     /locus_tag="QBF46_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDQ6674681.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDEEEDD"
     gene            complement(28066..28818)
                     /gene="fnr"
                     /locus_tag="QBF46_002509"
     CDS             complement(28066..28818)
                     /gene="fnr"
                     /locus_tag="QBF46_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDQ6674682.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(29013..29528)
                     /gene="ogt"
                     /locus_tag="QBF46_002510"
     CDS             complement(29013..29528)
                     /gene="ogt"
                     /locus_tag="QBF46_002510"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDQ6674683.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLNI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(29539..>30693)
                     /locus_tag="QBF46_002511"
     CDS             complement(29539..>30693)
                     /locus_tag="QBF46_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDQ6674684.1"
                     /translation="YASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVGRHPVAGLL
                     AAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMASSVVVLTI
                     VGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAIALMVIPEN
                     GILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPHLMIEPMKE
                     MAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLALLSSFLCM
                     FIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFL
                     QRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         7681 a         7377 c         7997 g         7638 t
ORIGIN      
        1 ttgcttactt tggcgcaggc gatgacgaag cacatatggt gtaccagttc tcgctgccgc
       61 cactggtgct gcatgcggtg caaaaacaga acgttgaggc gctttgtgcg tgggcgcaaa
      121 acctgacact accttccagc aacaccacct ggtttaactt cctcgcctct cacgatggca
      181 tcgggctaaa cccgctacgg ggcttgttgc ctgaaagcga aatattagag ctggtcgagg
      241 cgttacagca ggaaggtgca ttagtaaact ggaaaaataa tcccgacggt acacgcagtc
      301 cgtatgaaat aaatgtgacc tatatggatg cgttaagccg ccgtgagagt agcgatgaag
      361 aacgttgcgc caggtttatt ctcgcccatg cgattttgtt aagtttcccc ggtgtgccag
      421 cgatatatat tcaaagtatt cttggctcgc gtaatgatta cgcaggtgtc gaaaaactcg
      481 gatataaccg tgcgattaac cgtaaaaaat atcacagtaa agagataacc cgagaactga
      541 acgatgaagc tacattaagg catgcggtat atcatgagtt gtcgcgttta attacacttc
      601 gtcgcagcca taacgagttt catccggata ataattttac cattgatacg attaattcat
      661 ccgtaatgcg tattcaaaga agtaacgctg atggtaattg tctgactgga ttgtttaatg
      721 tcagtaaaaa tattcagcat gtaaatatta ctaatctgca tggtcgggat ctgattagtg
      781 aagttgatat attgggtaat gaaataacgc tgcgcccctg gcaggttatg tggattaaat
      841 aaaaaggaac atctcatgat taaatcaaaa atcgtgctgt tatcagcact ggtttcctgc
      901 gccctgattt caggctgtaa agaagaaaat aaaacgaatg tatccatcga atttatgcat
      961 tcttcggtgg agcaggagcg tcaggcagtt atcagcaaat tgattgcccg ttttgaaaaa
     1021 gaaaaccctg gcatcaccgt taagcaagtg cccgtggaag aagatgccta taacactaaa
     1081 gtcattactc tttcacgtag cggttcgctg ccggaagtga tcgaaaccag ccatgactac
     1141 gccaaagtga tggacaaaga gcagcttatc gatcgcaaag cggttgccac agtcatcagc
     1201 aacgttggtg aaggcgcgtt ttacgatggc gtactgcgta ttgtgcgtac cgaagatggt
     1261 agcgcatgga ccggtgttcc tgtcagcgcc tggattggcg gtatctggta tcgcaaagat
     1321 gtgctggcaa aagcggggct tgaggagccg aaaaactggc aacagctgct ggacgttgca
     1381 cagaaactga atgacccggc gaataaaaaa tacggcattg cgctgcctac agcagaaagc
     1441 gtgttgacgg aacaatcctt ctcccagttt gcgttatcca accaggctaa cgtctttaac
     1501 gccgaaggca aaatcaccct tgatacacca gagatgatgc aggcactgac ctattaccgc
     1561 gaccttgctg ccaacactat gccgggttct aacgacatca tggaagtgaa agacgccttt
     1621 atgaacggca ccgcgccgat ggcgatttac tccacctata tccttccggc tgtgattaaa
     1681 gaaggcgacc cggaaaacgt cggtttcgtg gtgccaaccg agaaaaactc tgcggtctac
     1741 ggcatgttga cctcgctgac cattaccgcc gggcaaaaga ccgaagagac ggaagcagca
     1801 gaaaaatttg tcacctttat ggagcaggca gacaacattg ccgactgggt gatgatgtcg
     1861 ccaggtgctg cgctgccggt gaataaagcg gttgtcacta ccgccacctg gaaagacaac
     1921 gacgttatta aggcgctggg tgaactaccg aatcagctaa tcggtgaact gccaaatatt
     1981 caggtctttg gcgcagtagg ggataaaaac tttacccgca tgggtgatgt gacgggttct
     2041 ggcgtggtga gctccatggt gcataacgtc accgtgggta aagccgatct ccctggcacg
     2101 ctgcaagcga gccagaaaaa gctggatgaa ctgatcgaac agcactaaac ccaggacagg
     2161 aatccgcaat gaacaggctt ttttcaggtc gttccgatat gccgtttgcg ctgctgcttc
     2221 tcgcgcccag cttattactg ctgggcggtc tggtggcgtg gccgatggtg tcgaatatcg
     2281 aaatcagttt tttacgtctg ccgctcaatc ccaacatcga gtcaacgttt gttggggtga
     2341 gtaactatgt gcgcattctc tccgatcccg gcttctggca ttcgctgtgg atgacggtct
     2401 ggtataccgc gctggtggtg gcgggtagca ccgtgctcgg gctggcggtg gcgatgtttt
     2461 tcaaccgcga gttccgcctg cgcaaaaccg cgcgttcgct ggtgatcctc tcctacgtaa
     2521 cgccgtccat ttcgctggtg ttcgcctgga aatacatgtt caacaacggc tacggcattg
     2581 tgaactacct cggcgtcgat cttctgcatc tctacgagca ggcaccgctg tggttcgata
     2641 atccgggcag tagttttgtg ctggtggtgc tcttcgccat ctggcgctac ttcccgtatg
     2701 cctttatctc gtttctggcg attttgcaga ccatcgacaa atcgctgtac gaagcggcag
     2761 agatggatgg cgctaatgcc tggcaacggt ttcgtatcgt cacgctgccc gcgattatgc
     2821 cggtcctggc gacggtggtg acccttcgca ccatctggat gttctacatg ttcgcggatg
     2881 tttatctgct gacgaccaag gtcgatattc tcggcgttta tctctacaaa accgcctttg
     2941 cctttaatga cttagggaaa gcggcggcga tctcggtggt gctcttcatc attattttcg
     3001 ctgtcattct gctgaccagg aaacgggtga acctcaatgg caacaaataa acgcacactc
     3061 agtcgaatcg gtttttactg cgggctggcg ctgtttctca tcatcacgct gtttccgttt
     3121 tttgtgatgc tgatgacctc gttcaagagc gcaaaagagg cgatctcgct gcatcctacg
     3181 ctgctgccgc aacagtggac gctggagcat tatgtcgaca tttttaaccc ggtgattttc
     3241 ccgtttgtcg actacttccg taacagcatg gtggtgtcgg tggtttcatc cgtggtggcg
     3301 gtatttctcg gcattctcgg ggcttacgcg ctttctcgcc tgcgctttaa aggccggatg
     3361 accatcaacg ccagctttta cacggtgtat atgttctctg gcattttgct ggtggtgccg
     3421 ctgttcaaaa tcatcaccgc gcttggtatt tacgacaccg agatggcgct gatcattacc
     3481 atggtgacgc aaaccctgcc caccgccgtg tttatgctga aaagctactt cgacaccatc
     3541 cctgatgaga tcgaagaagc ggcgatgatg gatggcctca aacgtctgca aattatcttc
     3601 cgtatcaccg tgcccctggc gatgtccggg ctgatatcgg tgttcgtcta ctgctttatg
     3661 gtggcatgga acgactatct gtttgcgtcg attttcctct ccagcgccag caatttcacc
     3721 ttaccagtcg gcctgaacgc gctgtttagt acgcctgatt acatctgggg acggatgatg
     3781 gcggcctcgc tggtgaccgc attgccggtg gtgattatgt atgcgctttc ggaacgtttt
     3841 attaagagtg gtttgaccgc cggtggcgtg aagggctaaa gcggccagtt tctttacaag
     3901 gagttttaca tgaaaaagtt agtagccaca gcaccgcgtg ttgctgcgct ggttgagtat
     3961 gaagatcggg cgattttagc caatgaagtg aagatccgcg tgcgtttcgg cgcaccgaaa
     4021 cacggaacgg aagtggtcga cttccgtgcc gccagcccgt ttatcgatga agactttaac
     4081 ggcgagtggc agatgttcac cccgcgtccg gcagatgcgc cgcgcggcat tgagtttggc
     4141 aaattccagc ttggcaacat ggtggttggc gacattatcg agtgcggcag cgacgttaca
     4201 gactacgcgg tgggcgacag cgtatgcggc tacggcccgc tctccgagac ggtcatcatt
     4261 aacgcagtga ataactacaa gctgcgcaaa atgccgcaag gcagctcctg gaaaaacgcc
     4321 gtctgttacg acccggcgca gtttgccatg agcggcgtgc gcgatgccaa cgtacgcgta
     4381 ggggattttg tcgtggtggt agggcttggc gcgatcggtc aaattgccat ccaactggcg
     4441 aaacgcgctg gcgcatcggt ggtaattggc gtcgatccta tcgcccatcg ctgtgatatt
     4501 gcccgtcgcc acggtgcgga tttctgcctt aaccccattg gcactgacgt aggcaaagag
     4561 atcaaaacgc tgaccggcaa gcagggtgcc gatgtgatta tcgaaaccag cggttacgcc
     4621 gacgcgctac aatcggcgct gcgcggtctg gcctacggcg gcaccatctc ctatgtcgcg
     4681 tttgccaaac catttgctga aggttttaac ctcggacgcg aagcgcattt caataacgcc
     4741 aaaattgttt tctcccgcgc gtgcagcgaa ccgaacccgg attatccgcg ctggagccgt
     4801 aagcgtattg aagaaacctg ctgggaactg ctgatgaacg gttatctcaa ttgcgaagat
     4861 ttaatcgacc cggtagtgac ctttgccaac agcccggaaa gctacatgca gtatgtcgat
     4921 cagcatccgg aacagagcat caaaatgggc gttacgtttt aattcaaagg aatgaaaaaa
     4981 tgaaaatagg cacacagaat caggcgttct tcccggaaaa tattctggag aaatttcgtt
     5041 atatcaaaga gatgggtttc gatggttttg agattgacgg caaactgctg gtcaacaaca
     5101 tcgaagaagt caaagcggcg atcaaagaaa ccggtttacc ggtgaccacc gcctgcggtg
     5161 gctatgacgg ctggattggt gactttatcg aagagcgtcg tcttaatggc ttaaagcaga
     5221 tcgaacgcat tctcgaagcg ctggcagaag tgggcggtaa aggtatcgtc gttccggcag
     5281 cgtggggcat gtttaccttc cgcttaccgc cgatgacttc accgcgtagt ctggacggcg
     5341 accgcaaaat ggtgagtgat tccctgcgcg tactggagca ggtcgccgcg cgtaccggaa
     5401 ccgtggtgta tctcgaaccg ttaaaccgct atcaggatca tatgatcaac accctcgccg
     5461 atgctcgtcg ttacatcgtc gaaaacaatc ttaaacatgt gcagattatc ggcgatttct
     5521 atcacatgaa catcgaagaa gataacctgg cgcaggcgct gcacgacaac cgtgacttgc
     5581 tcggtcatgt gcatattgcc gataaccatc gttaccagcc gggcagcggc accctcgatt
     5641 tccacgcgct gtttgaacag ctgcgcgccg ataactatca gggttatgtg gtgtatgaag
     5701 ggcgtatccg ggcagaagat cctgcccagg cgtaccgtga ttcgttggcc tggttgcgta
     5761 cctgctaaga ggtctttgtg aaaagtgcaa tgacaagctc tccgctgcgg gtcgcgataa
     5821 taggcgcagg ccaggttgcg gataaggtcc atgcttcgta ctactgcacc cgcaacgatc
     5881 tggaactggt ggctgtctgt gacagccgcc tttcccaggc gcaggcgctg gcagaaaaat
     5941 acgggaatgc atccgtgtgg gacgatccgc aggccatgct gctggcggtg aaacctgacg
     6001 tggttagcgt ctgctcacct aatcgttttc attacgaaca taccctgatg gcgcttgaag
     6061 cgggctgcca tgtgatgtgc gaaaaaccgc ccgccatgac gccaggtcag gcgcgggaaa
     6121 tgtgcgatac cgcgcgcaaa cagggcaagg tgctggccta cgactttcac catcgttttg
     6181 cgctcgatac gcaacagctg cgtgaacagg tgaccaacgg cgttttggga gagatttagg
     6241 ttaccaccgc ccgcgccctg cgtcgctgcg gcgttcccgg ctggggtgtc tttaccaata
     6301 aagaactgca gggtggtggc ccgctgatcg acatcggcat tcatatgctg gatgctgcga
     6361 tgtatgtgct gggctttccg gcggtgaaaa gcgtgaatgc gcatagcttt caaaagatcg
     6421 gcccgcaaaa gagctgtggt caatttggcg agtgggatcc ggcaacttac agcgccgaag
     6481 attcgctgtt tggcaccatt gaatttcata acggcggcat cctgtggctg gaaacgtcat
     6541 ttgcactaaa catccgcgaa cagtcgatta tgaacgtcag cttttgtggt gataaagccg
     6601 gtgcgacgct gtttccagca catatctaca ccgataacaa cggtgaatta atgacgctga
     6661 tgcaacggga aatggcagac gataaccgcc atttgcgcag catggaagcc tttatcaatc
     6721 acgtacaggg caagcccgtg atgatagccg acgccgagca ggggtacatc atccagcaac
     6781 tggtggcggc gttgtatcaa tccgcagaaa cagggacgcg tgtggaatta tgaccaggcc
     6841 agtaacgtta tcagaacccc atttcagcca gcataccctg aacaagtatg catcgctgat
     6901 ggcgcagggg aacggctatc ttgggctacg cgccagccat gaagaagatt acacgcgcca
     6961 gacgcgcggc atgtatctgg cggggcttta tcatcgggcg ggtaaaggtg aaatcaacga
     7021 actggtgaac ttgcctgatg tggtggggat ggagattgcc ataaatggtg aggttttctc
     7081 gttatcccac gaagcctggc agcgtgagct tgactttgcc agtggagaat tacgccgcaa
     7141 tgttgtctgg cgtaccagca acggcgcagg ttacaccatc gccagtcgtc gctttgtttc
     7201 ggcagaccaa ctcccgctca ttgcgctgga aatcactatt actccactgg acgccgacac
     7261 gtcagtgctg atttcaacag gcattgacgc cacgcaaacc aaccacggtc gccaacatct
     7321 cgacgaaacc caggtgcggg tgtttggtca gcatctgatg caggggatct acaccaccca
     7381 ggatggacgc agtgatgtgg ccatcagctg ttgctgtaag gtgagcggtg atgtgcagca
     7441 atgctatacc gccaaagagc gtcgcttgct gcaacacacc agcgcgcaac ttcacgcagg
     7501 cgaaacgctg acgctgcaaa aactggtgtg gatcgactgg cgggatgaca ggcaagctgc
     7561 tttagacgag tggggcagtg cgtcgcttcg ccagcttgaa atgtgtgcgc agcagagtta
     7621 cgaccaactt cttgcagcat caacagaaaa ctggcgtcaa tggtggcaga aacgtcgtat
     7681 cacggtaaat ggcggcgatg cgcacgatca gcaagcgtta gattatgcgc tttatcatct
     7741 gcgcatcatg acgcctgctc acgacgagcg cagcagcatt gcggcaaaag gcttaaccgg
     7801 cgaaggctac aaaggccacg ttttctggga tacagaagta tttttgctgc cgttccatct
     7861 gtttagcgat ccgacggttg cccgaagttt actgcgttat cgctggcaca acttgccagg
     7921 cgcgcaggag aaagcgcgac gcaacggctg gcagggcgcg ctatttccgt gggaaagcgc
     7981 gcgcagcggc gaagaagaga caccggaatt tgccgccatt aacattcgca ccgggctgcg
     8041 gcaaaaagtg gcctcggcgc aggcggaaca tcatctggtg gccgatatcg cctgggcggt
     8101 tattcaatac tggcagacca cgggggatga aagtttcatt gctcatgaag gcatggcgct
     8161 acttttggaa actgcaaagt tctggattag ccgcgcggtg agggttaacg accgtctgga
     8221 aattcatgat gttattgggc cagacgaata caccgaacat gtcaataata acgccttcac
     8281 cagctatatg gcgtattaca acgtccagca ggcgctgaat atcgctcgtc agtttggctg
     8341 tagtgacgac gcgtttattc atcgtgccga aatgttcctt aaagaactgc ggctgccaga
     8401 aattcagccc gacggtgttt tgccgcagga tgattcgttt atgactaagc cggcgattaa
     8461 tctggcgaaa tacaaagcgg cggcggggaa gcaaaccatt ctgctggatt attcacgcgc
     8521 agaagtgaac gagatgcaga tcctcaaaca agctgatgtg gtgatgctca attacatgct
     8581 gccggagcag ttctcagcgg catcgtgtct tgccaatctg caattttatg aaccgcgcac
     8641 tattcacgac tcgtcattaa gtaaagcaat ccacggcatt gttgccgcac gctgtggcct
     8701 gctgacccaa agttatcagt tctggcgcga ggggactgaa atcgatcttg gtgctgatcc
     8761 gcatagttgt gatgatggta tccatgctgc cgcaactggc gctatctggc tgggggcgat
     8821 tcagggtttt gccggggtga gcgtgcgtga cggtgaattg catctcaatc cagcgttacc
     8881 tgagcagtgg caacagttgt ctttccctct gttctggcag ggctgcgaat tacaggtcac
     8941 tctcgacgcg cagcgtattg cgattcgaac ttctgcgccc gtttcactgt gtttgaacgg
     9001 ccagcttata tccgtggctg aagaatctgt tttctgtttg ggtgatttta ttttgccctt
     9061 caatgggacc gctaccacgc atcaggagga tgaatgaaac tgcaaggggt aatttttgat
     9121 ctggatggtg taatcaccga taccgcgcat ctgcatttcc aggcgtggca gcagattgcc
     9181 gctgaaattg gcatcagcat tgatgcgcag tttaacgaat ccctaaaagg gatcagccgc
     9241 gatgagtctc tgcgacgcat tctgcaacac gggggcaaag agggcgactt taacccgcag
     9301 gagcggacgc aactggcgta tcgcaaaaat ctgctttacg tccactcact gcgcgagctg
     9361 acggttaatg ctgtgctgcc cggtattcgc aatttgatgg cagagctccg tgcacagcag
     9421 attccggtag ggctggcttc cgtctccctg aatgcgccga cgattttagc ggcgctggag
     9481 ctgcgcgagt ttttcacctt ctgcgcggat gcttcccaac ttaaaaactc gaaaccggat
     9541 ccggaaatct ttctcgctgc ctgtgccggg ctgggcgtgc cgccgcaggc atgtatcggc
     9601 attgaagatg cgcaggcggg cattgacgcc attaacgcca gcggtatgcg ctcggtgggg
     9661 atcggcgcgg gcttaaccgg ggcgcaatta ctattgcctt caacggactc cctgacctgg
     9721 tcgcggttat cggccttctg gcaaaacgta tagcaaagga atcaacatgg ctcagctttc
     9781 gttacaacat attcaaaaaa tctacgataa ccaggtgcat gtggtgaagg acttcaacct
     9841 ggaaattgcc gataaagagt tcatcgtatt tgtcggcccg tcgggctgcg gtaaatcgac
     9901 caccctgcgc atgattgccg ggcttgagga gatcagcggc ggcgatctgt tgatcgacgg
     9961 caaacgaatg aatgacgttc cagccaaagc acgcaatatc gcgatggtgt tccagaacta
    10021 cgcgttgtat ccgcatatga cggtttacga caacatggcg tttggcctga agatgcaaaa
    10081 aatcgccaaa gaggtgattg atgagcgggt gaactgggcg gcgcaaattc tcggcctgcg
    10141 tgagtacctg aaacgcaagc cgggcgcgct ttccggcggg caacgtcagc gagtggcgct
    10201 cgggcgggcg attgtacgcg aagcgggcgt gtttttaatg gatgaaccgc tctctaacct
    10261 tgatgccaag ctgcgcgtgc aaatgcgcgc agagatcagc aagctgcatc agaaactgaa
    10321 caccaccatg atctacgtga cccacgatca gaccgaagcg atgaccatgg cgacgcggat
    10381 tgtgattatg aaagacggga ttgttcagca agtaggtgcg ccgaaaaccg tttataacca
    10441 acccgcgaat atgtttgttg ccggatttat tggatcgccg gcgatgaatt ttattcgcgg
    10501 cacgatcgat ggcgataaat tcgttacgga aacgcttaaa ttaaccattc ccgaagagaa
    10561 attagcggtt ctgaaaacac aggaaagttt gcataagccc atcgtgatgg gaatacgccc
    10621 ggaagatatt catccggacg cgcaagagga aaataacatt tccgccaaaa ttagcgtggc
    10681 agaattaacc ggtgcggaat ttatgctcta caccacggtt ggggggcacg agttagtggt
    10741 ccgtgctggt gcgttaaatg attatcatgc aggagaaaat atcactattc attttgatat
    10801 gactaaatgt catttctttg atgcagaaac ggaaatagca attcgctaaa tacaggggga
    10861 aggcattccc ccaggataat acaaggaaca ataatgaaaa agttattacc ctgtaccgca
    10921 ctggtgatgt gtgcgggaat ggcctgcgca caggccgagg aaaggaacga ctggcacttt
    10981 aatatcggcg cgatgtacga aatagaaaac gtcgagggtt atggcgaaga tatggatggg
    11041 ctggcggagc cttcagtcta ttttaatgcc gccaacgggc cgtggagaat tgctctggcc
    11101 tattatcagg aagggccggt agattatagc gcgggtaaac gtggaacgtg gtttgatcgc
    11161 ccggagctgg aggtgcatta tcagttcctc gaaagcgatg atttcagttt cggcctgacc
    11221 ggcggtttcc gtaattatgg ttatcactac gttgatgaac cgggtaaaga cacggcgaat
    11281 atgcagcgct ggaaaatcgc gccagactgg gatgtgaaac tgactgacga tttacgtttc
    11341 aacggttggt tgtcgatgta taaatttgcc aacgatttga acacgaccgg ttacgctgat
    11401 acccgtgtcg aaacggaaac aggtctgcaa tataccttca acgaaacggt tgctttgcga
    11461 gtgaactatt atctcgagcg cggcttcaat atggacgaca gccgcaataa cggtgagttt
    11521 tccacgcaag aaattcgcgc ctatttgccg ctgacgctcg gcaaccactc ggtgacgccg
    11581 tatacgcgca ttgggctgga tcgctggagt aactgggact ggcaggatga tattgaacgt
    11641 gaaggccatg attttaaccg tgtgggttta ttttacggtt atgatttcca gaacggactt
    11701 tccgtttcgc tggaatacgc gtttgagtgg caggatcacg acgaaggaga cagtgataaa
    11761 ttccattatg caggtgtcgg cgtaaattac tcgttctgat aatgggctaa attgccggat
    11821 gcggcgcgag tactttatcc gatctataaa tgtaggccgg ataagatgcg ctagcatcgc
    11881 atccggcatt caggcaaggt agctggtatt tatttcagcg tcatatgcgt ggcaacggta
    11941 atattctgtg gtgacggttt tccagaaatt aagcggaata ataactcgca gctttgttga
    12001 cctaactcct gcgtcggaac atcgatgccg cccggtgcag gcgttaaaat aaatgacagc
    12061 gtttcattgc tataacccac caccgctaac tgctgcggaa tagcaatatt tttctctgct
    12121 gccgcacgat aaatgctcat taatttcagg ctgtcagtgg caaacaccgc ctcaggcaac
    12181 ggtgactggc ttaataattg ccgtgctgct tttaatgcag tttcatgggt ataaccgcca
    12241 tcaacaatcc attcatcacg cactgcaata ttatgcgcag ccaggctctg cttataacca
    12301 ttaacgcgat caaccgaaac atggacatca agcggagcat gcaggcaggc aatattttga
    12361 tgcccacttt caattaatgc atcggtcaac gcaatgctgt cgccaaaatt atcggtatcg
    12421 acagaataaa catgggcata ttgaccttca actttgccaa tcaccaccac cggaatatca
    12481 tatttatcga gttgggcaaa aaatgactca tccgctggcg aactgagcat aataatgcct
    12541 ttaatcattt tctgcttaat tttgctttcg catttttgca gatcttctgc cggattgtgc
    12601 gacgtttgta atatcacgtc gaaaccttct tcttcagctt tggcggtgat ggcatgcaaa
    12661 acttcagaga aaaacggatt acccgccgta gttttggtcg aacgggtaga aatcaccata
    12721 atggcatcaa aacccgaaga ggtcagcgcg cgggccagct tgtttggctg atactgtaat
    12781 tcttcaatgg cccgtaacac tttttcgcgc gcttccgggg agatattagt ttgcttattc
    12841 agcacgcgtg atacggtgga ttttgatacg cctgcaaccc tggcaatatc ataaatagta
    12901 ggggacatag gtcaggaact ccgtccgaac gtgaatgctg cacatcttat ggagttcgcc
    12961 ggtcgatgac aacacgggca gttgataatc aatggcctgg cccccacatt catatcctta
    13021 cgaatgattt tttttctggt cttcagagca gcacaggaca gcaatgaagc gacttaaaaa
    13081 tgaactcaat gcgctggtga atcggggtgt cgacagacat ctgcgcctcg ctgtaaccgg
    13141 acttagccgc agcggcaaaa cagcgtttat caccgcgatg gttaatcagt tgcttaatat
    13201 tcacgccgga gtacgtttgc cgctgttaag tgcggtgcgt gaagagcgcc tgctgggcgt
    13261 gaaacgcatt ccccagcgtg actttggcat tccgcgcttc acctatgacg aagggctggc
    13321 gcagttatat ggcgatccac ccgcctggcc aacgccaacg cgcggcgtca gtgaaatccg
    13381 cctggcgcta cgttttaaat cgaatgattc gctgctacgc cactttaagg atacctccac
    13441 gctgtatctg gaaattgtgg attatcccgg cgaatggttg ctcgacctgc cgatgctggc
    13501 gcaggactat ttaagctggt cgcgccagat gacgggctta ctcaatggtc agcgcggaga
    13561 atggtcggcg aaatggcgaa tgatgagcga agggctggac ccgctagcgc ctgccgacga
    13621 aaaccggctg gcagacattg ccgccgcgtg gaccgattat ctccaccact gtaaacagca
    13681 ggggctgcac tttattcagc ctgggcgctt tgtcttgccg ggagatatgg caggtgcgcc
    13741 cgcgctgcaa ttcttcccgt ggccggatgt cgatgtctgg ggcgagtcca aactggcgca
    13801 ggccgataag cacactaatg ccggaatgct gcgcgagcgg tttaattatt actgcgagaa
    13861 agtggtgaag gggttctata agaatcattt tctgcgcttt gaccgccaga ttgtgctggt
    13921 ggattgcctg caacctctca acagtgggcc acaggcattt aatgatatgc gtctggcgct
    13981 gacgcagctg atgcaaagtt tccactacgg gcagcgtacc ctgttcaggc gtttgttttc
    14041 gccggttatc gataagctat tgtttgctgc cactaaagcg gaccatgtga ccatcgatca
    14101 gcacgccaat atggtttcat tgctgcaaca actgattcag gatgcctggc aaaatgcggc
    14161 gttcgaaggg atcagcatgg attgcctggg gctggcgtca gttcaggcga ccaccagcgg
    14221 cattattgat gttaacggtg agaaaatccc ggcgctgcgc ggtaatcgac ttagcgatgg
    14281 cgcaccgctc actgtttatc ctggcgaagt tcccgcacgt ttgcctggtc aggcgttctg
    14341 ggataagcaa ggcttccagt ttgaggcgtt tcgtccgcag gtgatggatg tcgacaaacc
    14401 gctgccgcat attcgccttg atgctgcgct ggaattttta ataggagata aattgcgatg
    14461 accgaaccgt taaaaccacg tattgatttc gacggtccgc tggaggtcga tcagaatcca
    14521 aaattcaggg cgcagcagac ctttgacgaa aatcaggcgc aaaattttgc cccggccacg
    14581 ctcgacgaag cgcctgaaga agaggggcaa gttgaagcgg taatggatgc agcgttacgt
    14641 ccgaaacgca gcctgtggcg taaaatggtg atgggcggct tggctctgtt tggcgcaagc
    14701 gttgtcgggc agggtgtaca gtggacaatg aatgcctggc aaactcagga ctgggtggcg
    14761 ctgggtggat gtgccgctgg ggcattgatt atcggcgctg gcgtaggttc tgtggtaaca
    14821 gagtggcggc gcttatggcg cttgcgacag cgcgcccatg aacgcgacga agcgcgcgat
    14881 ttgttgcaca gccacggcac gggcaaaggc cgcgcatttt gcgaaaaact ggcgcagcag
    14941 gcgggtattg atcagtcgca tccggcgctg caacgctggt atgcctcaat ccatgaaacg
    15001 caaaacgacc gtgaagtggt cagcctgtat gcgcatttgg tccagccagt tttagatgcc
    15061 caggcgcggc gcgaaatcag ccgttcggcg gcggaatcaa cgttgatgat tgcggtcagc
    15121 ccgctggcgt tggtcgatat ggcgtttatc gcctggcgca atctgcgttt aattaatcgc
    15181 atcgccacgc tgtatggcat tgaactgggg tattacagcc gtttgcgtct gtttaagctg
    15241 gtattgctga atatcgcttt tgccggagcc agcgaactgg tgcgcgaagt ggggatggac
    15301 tggatgtcgc aagatctcgc tgctcgtttg tctacccgcg cagctcaggg aattggtgct
    15361 ggacttctga cggcacgact cgggattaaa gctatggagc tttgccgccc gctgccgtgg
    15421 attgacgatg acaaacctcg cctcggggat ttccgtcgtc agcttatcga tcaggtgaaa
    15481 gaaacgctgc aaaaaggcaa aacgcccagc gaaaaataat gcaatatcgg gtgctgaccg
    15541 gatatcttta cgccgaagtg cccgtttttc cgtctttgtg tcaatgattg ttgacagaaa
    15601 ccttcctgct atccaaatag tgtcatatca tcatattaat tgttcttttt tcaggtgaag
    15661 gttcccatgc gtctggaagt cttttgtgaa gaccgactcg gtctgacccg cgaattactc
    15721 gatctactcg tgctaagagg cattgattta cgcggtattg agattgatcc cattgggcga
    15781 atctacctca attttgctga actggagttt gagagtttca gcagtctgat ggccgaaata
    15841 cgtcgtattg caggtgttac cgatgtgcgt actgtcccgt ggatgccttc cgaacgtgag
    15901 catctggcgt tgagcgcgtt actggaggcg ttgcccgaac ctgtgctctc tgtcgatatg
    15961 aaaagcaaag tggatatggc gaacccggcg agctgtcagc tttttgggca aaaattggat
    16021 cgcctgcgca accataccgc cgcacaattg attaacggct ttaatttttt acgttggctg
    16081 gaaagcgaac cgcaagattc gcataacgag catgttgtta ttaatgggca gaatttcctg
    16141 atggagatta cgcctgttta tcttcaggat gaaaatgatc aacacgtcct gaccggtgcg
    16201 gtggtgatgt tgcgatcaac gattcgtatg ggccgtcagt tgcaaaatgt cgccgcccag
    16261 gacgtcagcg ccttcagtca aattgtcgcc gtcagcccga aaatgaagca cgttgtcgaa
    16321 caggcgcaga aactggcgat gctaagcgcg ccgctgctga ttacgggtga cacaggtaca
    16381 ggtaaagatc tttttgccta cgcctgccat caggcaagcc caagagcgag caaaccttac
    16441 ctggcgctga actgtgcgtc tataccggaa gatgcggtcg agagtgaact gtttggtcat
    16501 gctccggaag ggaagaaagg gttctttgag caggcgaacg gtggttcggt gctgttggat
    16561 gaaatagggg aaatgtcacc acggatgcag gcgaaattac tgcgtttcct taatgatggc
    16621 actttccgtc gggttggcga agaccatgag gtgcatgtcg atgtgcgggt gatttgcgct
    16681 acgcagaaga atctggtcga actggtgcaa aaaggcatgt tccgtgaaga tctctattat
    16741 cgtctgaacg tgttgacgct caatctgccg ccgctacgtg actgtccgca ggacatcatg
    16801 ccgttaactg agctgttcgt cgcccgcttt gccgacgagc agggcgtgcc gcgtccgaaa
    16861 ctggccgctg acctgaatac tgtacttacg cgttatgcgt ggccgggaaa tgtgcggcag
    16921 ttaaagaacg ctatctatcg cgcactgaca caactggacg gttatgagct gcgtccacag
    16981 gatattttgt tgccggatta tgacgccgca acggtagccg tgggcgaaga tgcgatggaa
    17041 ggttcgctgg acgaaatcac cagccgtttt gaacgctcgg tattaaccca gctttatcgc
    17101 aattatccca gcacgcgcaa actggcaaaa cgtctcggcg tttcacatac cgcgattgcc
    17161 aataagttgc gggaatatgg tctgagtcag aagaagaacg aagagtaagc gcgaatatgc
    17221 ctgatggtgc aataccatca ggcatattaa cttatgcttt cagtaccgcc agagctgctt
    17281 cgtaatccgg ctcggtggtg atttcatcca ccagctggct gaaaatcaca ttgtcatttt
    17341 cgtcaataac cacaacggca cgcgctgcca gacctttcag tgggccatca gcaattgcca
    17401 caccgtaggc ttgcaggaat tcagcgttac ggaaagtgga gagggtgata acgttgttca
    17461 gaccttctgc gccgcagaaa cgagactggg cgaacggcag atcggcagag atacacagca
    17521 caacggtgtt gtcgatctcg gttgccagtt ggttaaactt acgtactgat gcggcgcaaa
    17581 caccggtatc aatactcggg aaaatgttca gcactttgcg tttacccgca aactgaccga
    17641 gggtgacgtc agacagatct tttgccacga gagtaaaagt ctgcgctttg ctacccgcct
    17701 gcgggatgga gttggcgact gtaaccgggt tgccctggaa atgaacggtt tgtgacatga
    17761 ttatctttcc tgtttacata tagttaacgt cacacctagt ttatgctaac tgtcaataac
    17821 acagcaaacg ctatttgcgc ttaatccgca gaccaccgcg acaacaagga gtaaagatga
    17881 gaaccgttaa ggtattcgag gaagcctggc ccttacatac cccgtttgtg attgcccggg
    17941 gaagtcgcag tgaagcgtgc gtggtggtgg ttgaactgga agaagaaggc attaaaggca
    18001 ccggcgaatg cacgccgtat ccgcgttatg gggaaagtga tgcctcggta atggcgcaaa
    18061 ttatgagcgt cgtgccgcaa ttagagaaag ggctgacacg ggaggagttg caaaaaattc
    18121 tccctgccgg cgcagcacgt aatgcgctgg attgtgcatt gtgggatctg gcggcgcgaa
    18181 aacagcagca atcgctggct gatttgatcg gcataacgct tcccgagaca gtcaccactg
    18241 cacagacggt agtcatcggt acgcctgatc agatggccaa tagtgcatca acactctggc
    18301 aggccggcgc gaaattactg aaagtgaagc tggataacca tcttatcagt gagcggatgg
    18361 tggcaattcg cacagctgtg cccgatgcca cgctgatcgt tgatgcaaat gaatcctggc
    18421 gtgcagaagg gttggcggca cgttgccagc tattggcgga tttaggcgtt gcgatgcttg
    18481 aacaaccgct tcctgcgcag gacgatgcgg cactggagaa ttttattcat ccgttaccga
    18541 tttgtgctga tgaaagttgt catactcgta gcaatctgaa ggcgctgaaa gggcgctatg
    18601 agatggttaa cattaagctc gataaaaccg ggggtctgac ggaagcgctg gcgctggcga
    18661 ctgaagcgcg tgcacaaggt tttcgtctga tgctgggctg catgttgtgt acctctcggg
    18721 ccattagcgc cgctttaccg ctggtgccgc aggtcagttt cgccgatctt gacggaccga
    18781 cctggctggc ggtagatgtg gaaccggcgc ttcagttcac gacgggcgaa ttgcatcttt
    18841 aggatgccag cgcagcaagt ttgccatcgc aaagagatat ttttcgctgg cttcgtcgga
    18901 ggagatgggc ggaaactctg cggtgatgca atgcaagttg agatctgcac accaactgcc
    18961 gaaagagcca ggcgtttcat aaccgacgct ggttaccaga ggcaattcaa acgcctgggc
    19021 cagccattcg cctaattcgc tgtgtctggg atcgtcaata caggccagtg gatcgtggaa
    19081 tgagaccacc caggcaggtt gaatgcggtg tataagctgg cacagcgcct gggtttcagg
    19141 ttcggagcca ggtttgtcac ctgtcagtaa aacgacatca cgttcttcag cggcgctgtt
    19201 ccagcgataa actgtttcac cttccttcca gttcgctgcc ggaaagtttc ggtttaaatc
    19261 cacaccatta gcattggccc gtaaccctaa ctggcagccg tcaggattca cacacagcac
    19321 cacatgatgg cggcgcaaag aaggtgtcaa agtccgcaga gcacaggaga gcgtgacgac
    19381 cgaagagttt tcatcaccgt gagtgccagc cagaatcaaa ccactttcgc gactggctgc
    19441 aggggccgga aaccagatta acggcgcgcc caataatgaa cgtccgtaat gttctgttcc
    19501 gggcggaaat gcgccgcgtt cggcgcgtgg gcgggttacg gtcatgatcg tctctggttc
    19561 aggaagtatt acaggcagtg ttgtgcaaat tttgcggatt atcaaatcct tttcttcaac
    19621 gctatgtcat cgatcttgta tcacctttta gccggaagtt gtttgtattt tcattgatgt
    19681 gcagatatta gaattgtcgt aatgttatat acatggcgta ttttattctt actggttcta
    19741 cacatgttaa tcgcatctga ttgatcagtt caccttaata agtttaataa atcgtactgc
    19801 acatagtaca gtacgatggt agctatataa ttgatttaca tgaagaatgg cttatcgcca
    19861 tcagtcccgg gttgattgat gcctatattc tcaccaatgt gtttctcagg tttatcgatg
    19921 atatctgtaa ttaatgctgc gacactctta cgtgatacga tcgtaccttt gaatggttca
    19981 ttgcgtgagg taagctcata atcaataata tcttcatccg tcagccaggc agggcgaaga
    20041 attgtatatt ccagtccaga agcctcaatt gcatccgcag ccctgcggaa tggttttagg
    20101 ggctcaccaa taactgcatt attccattcg ccaaattttc ctggaacttc atcataaatg
    20161 ccgagggaaa gaacaaagat cagacgttta acatcacagg ctttcatcgc agcgatcact
    20221 gaatttgcct gaatatctaa atcttccccc gtaaggttgg catacacaat atcctgtccc
    20281 tgcatggcct gcttcagtgc tgcatgattc agtacatccc ccataataat ctggctatta
    20341 gttggatagg gtttgtgtat ttttgctggc tgacgagcaa ataaagtttg tttaatggtt
    20401 tgtttatctg ccagttgatt aatcacatgg cgggctatct gaccgccagc accaagaatg
    20461 agtacatttt tcataatatt tctcatgaaa cttatcccag atgggtgttg aaaaaaggaa
    20521 taactttttc cagcgcctcc tgaactgctt ccggtttgtc atacagttca taatgtgaga
    20581 aatcgggtaa tgaaactaac tgacgatcct gcgaaaccat cgcccggcca tagacttcca
    20641 gtccatcacg ataagcaccg aaggcaccga ctttttcacc aactactacc atcaccggct
    20701 gagtcagtaa cacttcagta aatgcgaagg catcccaggc cagcgttttc tgtgcatgtg
    20761 agaacaacat ttttgtcgca cctcccggtt gttgcccgcg tggtgtttta tagtagtcag
    20821 ttgcttcata aacatcgcgt tcggtcagtc catgggcttt tgctgcatcc aggctggcgg
    20881 gaagtaattc gttgatctgt aattctccac cacgagcctc ttttgttcgt tgtgacgcca
    20941 ttgcattcaa tacgccaatc ggatcataat tgctgaaacc ttcccgaaaa agacgcccga
    21001 tattaacacc cgtaatactc actactgctt taattcgttt ttctgtcagc gcggcattaa
    21061 tggcatagcc accaccccca cacactccca gcacgccaat acgttcagca tcaacatatg
    21121 gcagagttac tgcataatcg ataacacggc taatatcctc tacgcgctgt gtcggatctt
    21181 ctatccaacg aggctcccca ccagattctc cctggaaact ggcgtcataa gcgagaacga
    21241 catagccttt ttcagccaat gccttgccat agatattgcc agacgtctgc tccttacaac
    21301 tgccaaaagg atggacgctg ataatcgccg gatactgttt tgactcatca aaatttggtg
    21361 gaaactgaat attggcagca atatgccagg ccatatcact attgcggatt tgaatatgtt
    21421 tcattacaga gtttcctcaa agaacggagc taatactgaa attgcttcag ccacataggc
    21481 tttgccgtca taaagatcca tatggtttgc gccttcaaca atgtgatagc gtttatcctg
    21541 gcttgatgct cgatcgtaca ggtcgtcact catccatttg ctccctgcct ggctgcccgc
    21601 cacaatctgc gtcggctgag tcaggtacac ttccgccata tggtaagcat cataggtaat
    21661 aatctggtta aggctgcgca aagtagcgta acccggtgct gttggatact gcgcgcgagg
    21721 ggtgtggtaa tactcccagg cctgacgcag ttcttcgttc ggcgcatcgg actccttcat
    21781 tggtgccagt ggcataatgg cgtattctcc gctgctaata tcgctggttc tggcgtttga
    21841 acccgcttca acgtatggca gggcatcaat agatttcaca ttgttttccc aaccattacg
    21901 gaacatcgaa ccaatattga ctgcactaac ggtaccgatg gccttgatgc ggcgatcctg
    21961 aattgcagca ttggctgtat atcctgcacc ggcacaaatt cccatcgcac caattcgggt
    22021 attgtcgaca taagaaagcg ttgtcaggta atcaatcacg gcactgacgt cttcagtacg
    22081 aatgtatggg ttttctaact gacgcggctc gccgccactt tcaccctgat aagatgcgtc
    22141 ataagcaata gtgacaaatc ccttttccgc cagtttttcg gcataggtcc cggccgtttg
    22201 ttctttaacg cccccacctg ggtgagatac cacaattgcc ggatattgac gggtttcatc
    22261 aaattttgga ggaaaataga tcactgcaga caaagagatg gttggattat tgctgttagt
    22321 gaagctgact ttattattca tcattccgtt cctcatggag ttgttggttc gttttaacgg
    22381 ttggtgatat cactatagat attgatcatt aagttgatta gacccaaaat catgattaga
    22441 cctatcattt aaatgattaa tagattgtat ggagatactg aatgaagcgt gaagagatcg
    22501 ctgatctgat ggcgtttgtc gtcgttgcag aggagcgtag cttcactcga gcagcagccc
    22561 gcctgagcat ggcgcagtca gctttaagcc agatagtgcg ccgtatagaa gaacgattgg
    22621 gattgcggct tctgacccga accacgcgca gtgttgtacc cactgaagcg ggcgagcatc
    22681 ttttgtctgt tcttggccct atgttgcatg acatagattc agccctggca tccctgagcg
    22741 atctgcagaa ccgcccatcc gggacaatac gtattactac tgtagaacat gcagcaaaaa
    22801 cgatattgtt accagcaatg cgcacattcc tgaaatcgca tcctgaaatt gatattcagc
    22861 tcaccattga ttatggtttg accgatgtcg tttctgaacg ttttgatgca ggcgtccgtc
    22921 tgggtgggga gatggataaa gatatgatcg ccattcgaat cgggccagat ataccaatgg
    22981 ctattgttgg ctcaccggat tatttttctc gccgaagtgt tccaacgtca gtgtcacaat
    23041 taatagatca tcaggcaatt aatttgtatc ttcccacatc gggtacagca aatcgctgga
    23101 gattaatacg cggtggacgt gaagttcgtg ttcgcatgga aggtcagctt ttactgaata
    23161 cgatagacct gatcattgat gctgcaattg atgggcatgg attggcgtat ctaccttatg
    23221 atcaggttga gcgggctatt aaagaaaaaa aactgatacg tgttttggat aaattcacac
    23281 cagatttacc cggttatcac ctgtactatc cacaccgtcg acatgctggc tcggcattct
    23341 cattatttat agataggctg aagtataaag gtgctgttta gcactacttg ctgatacatt
    23401 aatttaattc ttctcttaac gtattctcag ttcctttcaa cgttttggtt attttttatt
    23461 cttcgtacaa tggcgacaga tgctgattat gataccgaaa actggtttga acatgcgaag
    23521 cccgaacgta gtgttcggtg ttctatgtgc tttgccgcat tttggcgtcg attatttaca
    23581 ctaaatctga tttaatatat tgatacataa aacattttat gcttcctgtg tcactttttt
    23641 gatggaagtt gtttgcattt ctttaaggcg aaacaaataa ttacgcatca attttaatgt
    23701 cggttagagg gaaacttatg aagcactctg tttcagtcac atgttgtgcg ctgttggtca
    23761 gcagcatttc tctttcgtat gctgcagaag ttccgagcgg cacagtactg gcagagaagc
    23821 aggagctggt gcgccacatt aaagatgagc ctgcgtcgct ggatcccgct aaagccgtgg
    23881 gcctgccaga gattcaggtc attcgcgatc tgtttgaagg actggtgaat cagaacgaga
    23941 aaggggagat tgtccccggc gttgcgactc agtggaaaag taatgacaac cgtatctgga
    24001 cttttaccct gcgcgataac gcaaaatggg cggatggcac accggtaacg gcgcaagatt
    24061 ttgtctacag ctggcaacgt ctggtggacc caaaaacatt gtcgccattt gcatggtttg
    24121 ccgcgctggc gggaatcaac aacgcacagg cgattattga tggtaaagct acgcctgacc
    24181 agcttggcgt caccgcagtt gatgcccata ctttgaaaat tcagcttgat aaaccgttgc
    24241 cgtggtttgt gaatttaacc gctaactttg ccttcttccc ggtgcaaaaa gccaacgtag
    24301 aaagcggtaa agagtggacg aaacccggaa atctgatcgg caatggcgct tatgttctta
    24361 aagagcgcgt agtcaatgaa aaactggtcg tggtaccgaa tacccattat tgggataacg
    24421 ccaaaacggt actgcaaaaa gtgaccttcc tgccaattaa tcaggaatcc gcagccacta
    24481 agcgttacct cgcgggggat attgatatca ccgaatcctt cccgaaaaat atgtatcaga
    24541 agctgttgaa ggatattccg gggcaggttt atacgccgcc gcagctcggg acctattatt
    24601 atgcgtttaa cacgcaaaaa gggccgacgg cagatcagcg cgttcgtctg gcattaagta
    24661 tgacgataga tcgccgcctg atgaccgaaa aagtattagg gacgggcgaa aagccagcgt
    24721 ggcattttac accagatgtt accgcgggat ttacgccgga accttcgccg tttgaacaaa
    24781 tgagtcagga agaactgaat gcgcaggcaa aaacattgtt gagcgcagct ggttatggtc
    24841 cgcaaaaacc gctgaagctg acgcttttgt ataacacttc agaaaaccat caaaaaattg
    24901 cgattgctgt agcatcgatg tggaaaaaga atcttggcgt agatgttaaa ttgcaaaatc
    24961 aggaatggaa aacctatatc gatagccgta acaccggcaa ttttgatgtt atccgcgcct
    25021 catgggtggg ggattataat gaaccctcca ctttcctgac attattaacg tcaacgcatt
    25081 caggaaatat ttcacgcttt aacaatccgg catacgacaa agttctggcc caggcatcga
    25141 cggaaaatac cgttaaagcg cgtaatgccg attacaacgc ggcagaaaaa atcctcatgg
    25201 agcaagcacc gattgcacca atttatcaat ataccaatgg acgattaatc aaaccgtggc
    25261 tgaaaggtta tcccattaat aaccctgaag atgtggcgta cagtcggact atgtatattg
    25321 tgaagcattg atgtgatggg aactggcgtt acccttgtgg ataacgccag tgatgtctga
    25381 tttagcgacc ctgttccggc ggtgtaatgt tatccatata cagcgtctgg ctggggaagg
    25441 caaagtccgc gccgtgtgac tgtacaatat cgataatctt caaataaacg tcttgctgtg
    25501 cagcaagcca ctcagcccat accgtggttt tggtaaagca ataaaccata atattcaatg
    25561 acgagtcagc aaactggttg aaataaacca gtaaggtttg tcgctggtcg atggccgggt
    25621 gatttttcag catctcacgt acggcttcga caataacgcc cacttttgcc gcatcctcat
    25681 aacgtaaacc aatggtcgtg gtaatgcgcc ggttggtcat tcgtcctggg ttttctacgc
    25741 tgatcgacga aaacagcgag ttcggtacgt acaatggacg attatcaaag gtcgtaattt
    25801 tggtaattcg ccagccaatt tccgctactg taccttcgat atttctgtcc ggtgaacgga
    25861 tccagtcgcc aatactgaaa ggacggtcga aatagagcat aatcccggaa aaaaagttac
    25921 tcagaatatc tttaccggcc ataccgacag ccagaccacc aataccacca aaggtcagca
    25981 agccagaaag gctcatgccg aaatgttcgc cataaagcag aacaagcacc acaataatgg
    26041 tgattttgat aatgcgcgac ataatccgcg cactggtgat atcgcgacct tttttaatct
    26101 gctgtttttc aaactgatta atcagcaaaa atagcttaat cgtcagaata accgcaatca
    26161 gggacgtaca gataaaatcg ataacgcctg gggtgataaa tttgagttta tagttttcta
    26221 taacataatt aataatgcta ccaacagcac tgataattat ggtgtagatt aaaaattgca
    26281 ccgcatggaa taaaaaacct tttcttttac gatttccacg gcgaaaccaa aagctcatca
    26341 gaatcaatgc tgcgcagcta ccgaaaataa tgaccagatt aagcgcatta tttgtaaaca
    26401 gttcagcgat cattgtttta tcaggctcct ccagataatt gtcgtcatgc cggaaacccc
    26461 tggcggggct attttaccgc gacaattcat gcagatcatc aatagtcagg gaaggaagta
    26521 gcaacattag ctaactaatc aatgcgttga ttgtaaatcc agctaagagg tgaggttttc
    26581 agagcagaca acggtgaaat gtcatggtat tgttacgttt aggtaacaag aaatttgtct
    26641 gcacaaggat tacatcatga ttatggcgaa actgaagtca gcgaaaggga agaaatttct
    26701 ctttggtttg ttggcggttt tcattattgc ggcgtcggtt gtgactcgcg cgactatcgg
    26761 cggcgttata gaacagtaca atattccgct gtctgagtgg acgacatcaa tgtatgtgat
    26821 tcagtcatcg atgatttttg tttatagcct ggtttttact gtgttgctgg caatcccgtt
    26881 gggaatttat ttccttggcg gcgaagagca gtaagtaaaa aataggcccg ataactcggg
    26941 ccttgtcagt tattgaagag tcgttaatcg tcctcttctt catccagttc aacgggtgtc
    27001 tgatactggt caggtttaat gaccaacaga tcgcagcgaa gatgatcaat cacctgttcc
    27061 gccgtgttgc cgaggaatgc tgctgaaata ccggtgcgtc ctaccgtgcc cagaaccaca
    27121 atccccgcct gtaagtgctc cgccaaatca ggaatcacct cttctggcag acctttttct
    27181 acgtgcgtca tgttttcatt aatgccgaat ttctgccgca gagctttcat tgccagcaaa
    27241 tgttgcccac gaatggcatc gttataaacg ctcggatcaa attccggcag ttcaatcgcg
    27301 atattaattg gcgttactgg gtaagcgcca accagatgaa cttcggtatg gttgacttgt
    27361 tctgccagtt cgatcgtctc tttgaccagt ttttcattga gcgcattatg atacggctct
    27421 tcactggcga gattcaccgc caccagcgcc ttacctcctt ccggccacgg ctggtctttc
    27481 accatccaca ccgggcttgg gcatttgcgt aacagatgcc agtccgttgg cgtaaaaatc
    27541 accgcttcca gacggtcatg ttggtgcgcc atttttagca ccaaatcgtg cccgccgctg
    27601 atcacttcct gaatgatggc ttcgaaagga cggttatgcc agaccacttt aatttcaatg
    27661 ggaacgccag cattgagata atattttgcc tgctcgtgga tccaggctgt acgctggctg
    27721 atgacgccct gacgcatagc ggtacgttcg tccggggaaa gcagggtggt catttcgtat
    27781 gagaagtcat agatcggcaa aaaggcttta attttgccac caatccgttg atgtaaataa
    27841 acagctcgcc gcaatgctgg ttggtcgtcc tggttaggat cgataacaac gagcatgttc
    27901 tgatacatag ccatacaggg tctccttaca acaactgtca acgcagtttg taattaaaag
    27961 attaacccat atctggtgaa tgaaacagtg atgaaccttc tgccagatca ataaatcaga
    28021 aaaatttaat gatatgacag aaggatagtg agttatgcgg aaaaatcagg caacgttacg
    28081 cgtatgacca gcaagctggg ccagcgcatc gttattttcg atggtgatgt atttaccttt
    28141 gactgccagc ataccgcttt tctggaagcg acccagcaga cgactgatgg tttctaccgt
    28201 caggcccaga tagttaccga tatcgccgcg agtcatcgtc aggcggaatt cacgagggga
    28261 gaagccgcgt tgggcaaaac gacgggacag gttgtagatg aatgcagcca gacgttcctc
    28321 ggcatttttc ttcgacaaca gcaggatcat gtcctgatcg cctttgattt caccgctcat
    28381 cagacgcatc atctgctgac gcagattcgg cattttaccg gacaaatcgt ccagcgtttc
    28441 gaacgggatt tcacatacca ttgaggtttc cagcgcctgc gcgaagctcg ggtgatgacc
    28501 gctgccgatg gcgtcaaatc ccaccaggtc gcctgctaaa tggaaaccag tgatttgctc
    28561 gtcgccttgc tcagtgatgg tataactttt aatcgtaccg gagcggatgg cataaagcga
    28621 tttaagttca tcaccagcct taaacagcgt ctggcctttc tgaataggct tcttccgctc
    28681 aatgatatta tcaagctgat caagctcatg ttcgttgagt gtgaacggga tgcaaagctg
    28741 gctgatgctg caatcctggc aatggatagc acaaccgcca gactgaatgc gccgtataat
    28801 tcgcttttcc gggatcatag gtctgctcaa gccgtaattg atatttgtca attttaacat
    28861 ctttttaggg agcaagtaag tctaagcaaa ccttaacagc agagaattcc gatattagat
    28921 gtaaatatat gtctatctat ttgaaaaccc ttaagttgtt aagggtaact ttacataaaa
    28981 gtgtgaacaa gctggcacaa attgtttaat gtttacagca aaagataacc ttcatggcgt
    29041 aataaccact cttttcgctg aactccgcct gcatatccgg tcatggtgcc gtttcggcca
    29101 ataacccgat ggcaaggtac tacaatgctg atgggattcg atccgtttgc cgcaccaacg
    29161 gcacgcgccg cgccagggcg gcccaattgt tcagccagtt ggccgtaatg cattacctgc
    29221 ccgcagggga tagtgcgtag tgttttccag acttcgcgct gaaatggcgt cccccctgtg
    29281 gcagtgggaa gcgtatcaat aatgctaaga ttaccggcaa aataatcacg aagcttgtcg
    29341 cttaaaccgc ctgggttggt ggcagaaatg cgctcatagc cttctttgcg ataatggatg
    29401 ttcagcagct gcaccatgcg ttcgctgtac tcttcccatt caaccgcccg caggcgaaat
    29461 tgctcatcgc aaatcaccca cagtggaccc agtggcgtgg caattttttc ttcaagtaat
    29521 ctcagcatcc gttctctctt aagacaaacg tgggtaaatc cccggaccta tcggcagacc
    29581 aacaagatac cacgccagca acatcagcag ccataccacc aaaaagataa gcggataggg
    29641 caagactaac gaatagtaag tacccagttt cgcgtctggt ttgtagcgtt gcaggaatcc
    29701 aagaaacagt ggaacaaaag gagataccgg cgctaaaggc aatacggatg agtcggcaat
    29761 acgaaagagg atttgcgcaa atgccgggtg aaagccaagt agcataaaca ttggtacgaa
    29821 aatgggggcc agaatcgacc agattgcgga accgctggcg ataaacatgc ataagaaaga
    29881 ggaaagcaac gccagaccga caaacgccgg gatgccgcta agccctgaac tttccagtat
    29941 atcggtcagc cccacggcga tgaatttccc catgttgctc cagttaaaca tggcgacaaa
    30001 ttgggcgagg ggaaaaacca tcacgataaa tcccgccatc tctttcatcg gttcaatcat
    30061 taaatgcggt aaatccgcct gacgtcgaat tgtgcgggta gcgatgccat aagccagcga
    30121 gacaacaaag aaaaaaagaa tgatcagtgg cacgatacct ttaataaagg gtgatggcat
    30181 cacggtgtga ttaatcggat cgcgcaatat cccgttttcc gggatcacca tcagcgcaat
    30241 cgcagcaata aaaagtagcg atacgacacc tgctatgcgt aaaccaaaac gctgactttc
    30301 ggtcaatgtc tgcagtttct catcgctgtt tccctgccat tgacctaacc gtggctcgat
    30361 gattttgtca gttatcaggc cgccaacaat cgtcagtacg actacggagc tggccataaa
    30421 ataccagtta tcaattacac tgacgtgcat ttgcggattg aacgcagccg ccgcttccgt
    30481 gctgatcccc gacagcaaca cgtcggttgt gacaatcagt aaattagccg taaagccgca
    30541 acctacgcct gcaatagcag ccagtaaacc tgcaactgga tgcctgccca ccgccagaaa
    30601 aatcagcgca cccatcggtg gcatgatcac taacgccgca tcggaagaaa tgtggctgaa
    30661 aaaagcaata aacagcacca tataactggc gta
//
LOCUS       DAOFQD010000057        29463 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.057, whole genome shotgun sequence.
ACCESSION   DAOFQD010000057 DAOFQD010000000
VERSION     DAOFQD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.057"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            208..1305
                     /gene="mltA"
                     /locus_tag="QBF46_002512"
     CDS             208..1305
                     /gene="mltA"
                     /locus_tag="QBF46_002512"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDQ6674685.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1544..2350
                     /gene="tcdA"
                     /locus_tag="QBF46_002513"
     CDS             1544..2350
                     /gene="tcdA"
                     /locus_tag="QBF46_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDQ6674686.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2401..2844)
                     /gene="csdE"
                     /locus_tag="QBF46_002514"
     CDS             complement(2401..2844)
                     /gene="csdE"
                     /locus_tag="QBF46_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDQ6674687.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2844..4049)
                     /gene="csdA"
                     /locus_tag="QBF46_002515"
     CDS             complement(2844..4049)
                     /gene="csdA"
                     /locus_tag="QBF46_002515"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDQ6674688.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4241..4468
                     /locus_tag="QBF46_002516"
     CDS             4241..4468
                     /locus_tag="QBF46_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDQ6674689.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4819..5736
                     /gene="gcvA"
                     /locus_tag="QBF46_002517"
     CDS             4819..5736
                     /gene="gcvA"
                     /locus_tag="QBF46_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDQ6674690.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5755..6150
                     /locus_tag="QBF46_002518"
     CDS             5755..6150
                     /locus_tag="QBF46_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDQ6674691.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6143..7243
                     /gene="rlmM"
                     /locus_tag="QBF46_002519"
     CDS             6143..7243
                     /gene="rlmM"
                     /locus_tag="QBF46_002519"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDQ6674692.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7287..8018)
                     /gene="fucR"
                     /locus_tag="QBF46_002520"
     CDS             complement(7287..8018)
                     /gene="fucR"
                     /locus_tag="QBF46_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDQ6674693.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8076..8498)
                     /gene="fucU"
                     /locus_tag="QBF46_002521"
     CDS             complement(8076..8498)
                     /gene="fucU"
                     /locus_tag="QBF46_002521"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDQ6674694.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8500..9918)
                     /gene="fucK"
                     /locus_tag="QBF46_002522"
     CDS             complement(8500..9918)
                     /gene="fucK"
                     /locus_tag="QBF46_002522"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDQ6674695.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGIGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10027..11802)
                     /gene="fucI"
                     /locus_tag="QBF46_002523"
     CDS             complement(10027..11802)
                     /gene="fucI"
                     /locus_tag="QBF46_002523"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDQ6674696.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QQIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11835..13151)
                     /gene="fucP"
                     /locus_tag="QBF46_002524"
     CDS             complement(11835..13151)
                     /gene="fucP"
                     /locus_tag="QBF46_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDQ6674697.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13698..14345
                     /gene="fucA"
                     /locus_tag="QBF46_002525"
     CDS             13698..14345
                     /gene="fucA"
                     /locus_tag="QBF46_002525"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDQ6674698.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14373..15521
                     /gene="fucO"
                     /locus_tag="QBF46_002526"
     CDS             14373..15521
                     /gene="fucO"
                     /locus_tag="QBF46_002526"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDQ6674699.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTHKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15576..16331)
                     /gene="xni"
                     /locus_tag="QBF46_002527"
     CDS             complement(15576..16331)
                     /gene="xni"
                     /locus_tag="QBF46_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDQ6674700.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16443..17810)
                     /gene="sdaB"
                     /locus_tag="QBF46_002528"
     CDS             complement(16443..17810)
                     /gene="sdaB"
                     /locus_tag="QBF46_002528"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDQ6674701.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17868..19157)
                     /gene="sdaC"
                     /locus_tag="QBF46_002529"
     CDS             complement(17868..19157)
                     /gene="sdaC"
                     /locus_tag="QBF46_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDQ6674702.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19714..21078)
                     /gene="ppnN"
                     /locus_tag="QBF46_002530"
                     /gene_synonym="ygdH"
     CDS             complement(19714..21078)
                     /gene="ppnN"
                     /locus_tag="QBF46_002530"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDQ6674703.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21190..22038)
                     /gene="queF"
                     /locus_tag="QBF46_002531"
     CDS             complement(21190..22038)
                     /gene="queF"
                     /locus_tag="QBF46_002531"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDQ6674704.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQYITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22106..22651
                     /gene="syd"
                     /locus_tag="QBF46_002532"
     CDS             22106..22651
                     /gene="syd"
                     /locus_tag="QBF46_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDQ6674705.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23273..23602
                     /locus_tag="QBF46_002533"
     CDS             23273..23602
                     /locus_tag="QBF46_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDQ6674706.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23602..24384
                     /gene="truC"
                     /locus_tag="QBF46_002534"
     CDS             23602..24384
                     /gene="truC"
                     /locus_tag="QBF46_002534"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDQ6674707.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24402..24851
                     /locus_tag="QBF46_002535"
     CDS             24402..24851
                     /locus_tag="QBF46_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDQ6674708.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS"
     gene            25286..26638
                     /gene="gudP"
                     /locus_tag="QBF46_002536"
     CDS             25286..26638
                     /gene="gudP"
                     /locus_tag="QBF46_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDQ6674709.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWMMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26640..27980
                     /locus_tag="QBF46_002537"
     CDS             26640..27980
                     /locus_tag="QBF46_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase-related protein"
                     /protein_id="HDQ6674710.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGDVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYVENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            28001..29341
                     /gene="gudD"
                     /locus_tag="QBF46_002538"
     CDS             28001..29341
                     /gene="gudD"
                     /locus_tag="QBF46_002538"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDQ6674711.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYKKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
BASE COUNT         7213 a         7158 c         7861 g         7231 t
ORIGIN      
        1 ccgtggatgc gcactatact ccgctcgcgt tttcatgcaa cctttttttt accccgatca
       61 cggatttagc atgattttga acaaaaacaa accactaaag cctatttttt gtacaaaatt
      121 tgcgtcaggg agaggctgct catttcatta aacgcggcgg tttgttatct tcgttgcgcc
      181 ttatttttta acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg
      241 ggcacggttg tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa
      301 tataaagacg ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc
      361 gcgccgatta acgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg
      421 cgtctgtatg gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc
      481 ggtgataccc gcaatatgcg ccagttcggc attgatgcct ggcagatgga aggtgccgac
      541 aactatggta acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc
      601 cgccagggcg agttccagta tcctatttac cgtatgccgc caaaacgtgg tcgtctgccg
      661 tctcgtgcgg agatctacgc gggggcattg agtgataaat atattctcgc ttacagtaac
      721 tccctgatgg ataacttcat tatggatgtg cagggtagtg ggtatatcga ctttggtgat
      781 ggcagtccgc ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt
      841 ggtaaggtgc tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt
      901 cgtcactggg gcgaaacaca cagtgaagcc gaggttcgcg agctgctgga acagaacccg
      961 tctttcgtct tctttaaacc gcaatctttt gctccggtga aaggggcaag tgcggtgccg
     1021 ctggttggcc gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg
     1081 ctggcagaag tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt
     1141 ctgatggtgg cgctggatgt cggtggcgca atcaaaggcc aacacttcga tatctatcaa
     1201 gggatcgggc cggaagccgg gcaccgcgca ggttggtaca accactatgg acgtgtctgg
     1261 gtgctgaaaa ccgccccggg cgcaggtaac gtctttagcg gctgatgtga tattctgtat
     1321 gccgttttta cggatatgac agggtgaggg gtaacctcac atcctgatta ttgactaagt
     1381 gcgcgttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa tcatgcaaat
     1441 ccaaaatatt gcaggagtta tgtaggcctg ataagcgtag cgcatcaggc atttatgcgt
     1501 ttagggtgag ggataacctc gcccttttta attctgaggt gctatgtctg tggtaattag
     1561 tgatgcatgg cgtcagcgtt ttggtggcac agcgcgtctg tatggtgaaa aagcgttgca
     1621 actgtttgct gacgcgcata tttgtgtggt tggtatcggt ggtgtcggtt cctgggcggc
     1681 ggaagcgctg gcgcgcacgg ggattggcgc aatcacgctt atcgatatgg atgatgtgtg
     1741 cgtcaccaat accaatcggc aaattcacgc tctgcgcgat aacgtcgggc tggcaaaagc
     1801 ggaagtcatg gcggagcgta ttcgccagat taacccggag tgccgtgtaa cggtggtgga
     1861 tgatttcgtg acgccggata acgtagcgca gtatatgagc gtgggttatt cgtacgtgat
     1921 tgatgccatt gatagtgtac ggcccaaagc ggcgctgatt gcttattgtc ggcgcaataa
     1981 aatcccgctg gtcacgaccg gtggcgcggg tgggcagatt gacccgacgc agattcaggt
     2041 taccgatctg gcgaaaacga ttcaggatcc gctggcggcg aagttgcgcg agcgcctgaa
     2101 aagcgatttt ggcgtagtga aaaacagtaa aggtaagctc ggcgtggatt gcgtgttttc
     2161 tactgaagcg ctggtgtacc cgcagtcaga cggtacggtg tgtgcgatga aagccacggc
     2221 agaagggcca aagcggatgg attgtgcatc tggatttggc gcggcaacga tggtgaccgc
     2281 cacctttggg tttgttgcgg tttctcatgc gctgaagaag atgatggcga aagcggcgcg
     2341 tcagggttaa acaatcaggt cggatgagac gcgaacgtct tatccgaccc ggttctgtgc
     2401 ttaaacctgc ttcgtcgcag cgataatcgc ctcgcttaac gcatttaacc cctggctacg
     2461 tgaggcgcta agctgcgcac gcaatcccag ctcatcaaac aatgccagcg gcgactgtgc
     2521 ctgcaattcg gcggcggttt tcccctcaac ggcagtcaac agcaccgcca gcaggccgcg
     2581 cactatacgt ccttcgctgt cgccaaagaa gtgcatttta ccgttttcag ccacggtata
     2641 tcccagccag acgcggtttt cgcatccggc aatctcttta gcctgcgctt ttaactcgtc
     2701 tggcaaagcc ggaagctgtt tccccagcat gatcaactgg cgatatttat cttcccattg
     2761 cgtcaacggt gcgaaggtat tgcgtaacgt ttctgcggtt acggttgtgc cgaacggatg
     2821 tccggcgaat tgcgggttcg tcattaatcc accaataatt ccagcgcgcg gtcaacggca
     2881 ttcaccagcg catccacatc actctttgta ttatatggcg caaaagaggc gcgcagtgtg
     2941 ccggttacgc ctaattctgc cagtagcggc tgagcgcaat gctgcccggc gcgcagggca
     3001 ataccgtact ccgccagcag cgtcaccata tcgctgtgat gaacgccagc aaaatcaaag
     3061 gccagcaggc tggaatcctg gcagcggaat gaacgaaagc ctggacgttt tgccagcgca
     3121 tcttccgcca gcgtcgccag gctgcgactc caactttctg cctggttgat atcgtaatct
     3181 gccagccatt ccagcgccgc gcttaatcct atgacaccag ccacatttgg cgttcctgct
     3241 tccagtttcc acggcgcaga ttgagtcgtg aagccgtcaa aactcacttc gtgaatcatt
     3301 ttgccgccac ccagccaggg cgacatcgct tccagcagtt ctgatttgcc atacagcacg
     3361 ccgatacccg tcgggccata cagtttgtga ccagagaaag catagaaatc gatatccagt
     3421 tgctgaacat ctgcggggaa atgcaccgcc ccctgagcac catcaaccat caccaccatc
     3481 ccggctgaat gagcaaaggt aatcgctcgc gccagatccg ggcaaccgcc agtcacgttc
     3541 gacatctgac ccaacgccag aatccgacta cggggagtaa tcagttctgg caacaaatcg
     3601 acatccggca gtcgctgcgc attaagcggc aatttcacca ctttggctcc agtttgttgg
     3661 gcgaccatca gccaggggac gaggttagcg tggtgttctg ccacgctgac aataatctca
     3721 tcgcccggtt gcagacgcgg acgcgcatag cattgtgcca ccatgttgat ggattcagtg
     3781 gtgccgcgcg tccagacgat agttttatca tccgatgcat tcagtaattg tgccactttt
     3841 tcccgcgcag cttcataacg cgcggtcagg cgttgggctt cggcaaactg gctgcgatgg
     3901 acgtttccgg cgctcaggct gtaaaactgt cgagtggctt caaccacggc ttcaggttta
     3961 agcgcggtcg cggcgctgtc gagatagacg cccgcatcct gtagtgcggg aaactgggcg
     4021 cgaaactgcg cgggattaaa aacgttcatg gtactcctcg gcttgatgca ccgatcgtga
     4081 cgcaaaattc actttgatac aaggagagtg ctaaccatca gaattatctg gatatcctgg
     4141 cgaagccgcg taagtaacat atgctgaata gtgttaggcg ttaattttag cctgttaatc
     4201 aatactatta agatttatta atcgctttaa ggagaagatg atgaaaaaga ctgccgcaat
     4261 tatttctgcc tgtatgctga cttttgccct gagcgcctgt tccggttcga actatgtgat
     4321 gcacaccaat gacggacgta ccatcgtctc tgacggcaaa ccacagactg ataacgatac
     4381 cggtatgatt tcgtataaag acgctaatgg caacaaacag cagatcaacc gtactgacgt
     4441 gaaagagatg gtcgaactgg atcagtaatt cgcgatcgca aggtaaaaaa aagcaccgca
     4501 attaggcggt gctacattaa tcactatgga cagacagggt aaatgtacag gaagtgaaaa
     4561 aaggtagctt tgctaccatg gtctgaatcg cagaccaatt gcaaacacaa caacacaaca
     4621 tcacaaccgt aagccaaaag ttcaccagaa cacgcattcc gataaaactt ttcgttccgg
     4681 ctcaggaagt gccgccacta taggtatttg ctggtagaag ctcaacggac aatttataat
     4741 ggctcagatt aaaaaaacta ataggttaca tagtgtgatc taattgttga attcatttaa
     4801 catcaaagtt taatagccat gtctaaacga ttaccaccgc taaatgcctt acgagttttt
     4861 gatgccgcag cacgccattt aagtttcact cgcgcagcag aagagctttt tgtgacccaa
     4921 gccgcagtaa gtcatcaaat caagtctctt gaggattttt tggggctaaa actgttccgc
     4981 cgccgtaatc gttcactcct gctgaccgag gaagggcaaa gctatttcct cgatatcaaa
     5041 gagatatttt cgcaattaac cgaagcgacg cgtaaactcc aggcccgtag cgccaagggg
     5101 gcgttgacgg tcagtttact ccccagtttc gccattcatt ggttggttcc gcgactttcc
     5161 agctttaatt cagcttatcc gggaattgac gttcgaatcc aggcggttga tcgtcaggaa
     5221 gataagctgg cggatgatgt tgatgtggcg atattttatg gtcggggcaa ctggccgggg
     5281 ctacgggtgg aaaaactgta cgccgaatat ttattgccgg tgtgttcgcc gctactgctg
     5341 actggcgaaa aacccttgaa gacaccggaa gatctggcta aacatacgtt attacatgat
     5401 gcttcgcgcc gtgactggca gacatatacc cgacagttgg ggttaaatca tatcaacgtt
     5461 cagcaagggc caatttttag ccatagcgcc atggtgctgc aagcggctat ccacgggcag
     5521 ggagtggcgc tggcaaataa cgtgatggcg caatctgaaa tcgaggccgg acgtcttgtt
     5581 tgcccgttta atgatgttct ggtcagtaaa aatgcttttt atctggtttg tcatgacagt
     5641 caggcagaac tgggtaaaat agccgccttt cgccaatgga tcctggcgaa agccgctgct
     5701 gaacaagaaa aattccgctt tcgttatgaa caataattta cgtagggtac gaccatgacc
     5761 agccgtttta tgctgatttt cgccgccatt agcggcttca tttttgtggc tctgggcgct
     5821 tttggcgcgc atgtgttaag taaaaccatg ggggccgttg agatgggctg gatccagacc
     5881 ggcctcgaat accaggcgtt tcatacgctg gcgatcttag gtctggcggt ggcaatgcag
     5941 cgtcgcatca gtatctggtt ttactggagt agcgttttcc tcgcgttagg cacggtgctg
     6001 ttcagcggca gcctttattg cctggcactg tcccatctgc gtttgtgggc gtttgtcact
     6061 ccggttggcg gcgtgagctt ccttgcgggc tgggcgttaa tgttagttgg tgctatccgt
     6121 ttaaagcgca agggcgtaag tcatgaataa ggttgtattg ctgtgccgtc cgggctttga
     6181 aaaagagtgc gccgcagaaa ttaccgataa agccggccag cgggaaattt tcggttttgc
     6241 ccgcgtgaaa gagaatgcgg gttatgtcat ttatgaatgt tatcaacctg atgatggcga
     6301 taagttaatc cgtgagctgc cgttcagttc attaattttt gcccgccagt ggtttgtggt
     6361 gggggaactc ctgcagcatt tgccgccaga agatcgtatt acccccattg tcggcatgtt
     6421 acagggcgta gtagagaagg gcggtgaact gcgtgttgaa gttgccgata ccaacgaaag
     6481 caaagagttg ctgaaattct gccgtaaatt caccgtgccg ctacgcgctg ccttgcgcga
     6541 tgccggggtg ctggcgaact atgaaacgcc gaagcgtccg gtagtgcatg tattcttcat
     6601 tgcaccaggc tgctgctata ccggttactc atacagcaac aataattcgc cgttctatat
     6661 gggcattccg cgcctgaaat ttccggcaga tgcgccgagt cgttctacgc tcaaactgga
     6721 agaggcattt catgtgttta ttcctgcgga tgagtgggat gaacgcctgg cgaacgggat
     6781 gtgggcggtg gatttgggcg cttgcccagg cggctggacc taccaactgg tgaagcgcaa
     6841 catgtgggtt tattccgtcg acaacggccc gatggcgcaa agtctgatgg ataccgggca
     6901 ggtgacgtgg ctgcgggaag acgggtttaa attccgtccg acacgcagca atatctcctg
     6961 gatggtatgc gatatggttg aaaaaccggc gaaagttgcg gcattgatgg cgcagtggct
     7021 ggttaatggc tggtgtcgtg aaaccatttt caacctcaaa ctgccgatga aaaaacgcta
     7081 cgaagaagtg tcacacaatc tggcgtatat tcaggcacag cttgatgaac atggcataaa
     7141 tgctcagatt caggcgcggc agttgtatca cgatcgcgaa gaagtgacgg tgcacgtccg
     7201 ccgcatctgg gctgcggtgg gtggtcgtcg cgacgagcga taacaggcaa ggccggggcg
     7261 tcgtctccgg cctgctaccc tttccgtcag gctgttacca aagaagttgc aacctggcgc
     7321 tcatcagaaa taatgtgcgt tacctcatcc agatgcccga tgcgggcttc ccctgaacga
     7381 ttaaatttac ttttatcaat caataacaac gattgcgcgg cacgttttaa tagcatcgat
     7441 ttgtaatcag cgttgatcgc attggagtcc cacagtgcgc cgctgctatc gatcccttca
     7501 caagaaaaaa taaacagatc gatttccagc gatttaagtt gggaaatcag cgagggattg
     7561 acgtaacagc catatttgcg ctcaagtgtg ccgccggaac tgatcagttg aatgcgttcg
     7621 cgtttaccga gttcatggca aatcggatgg ctattggtga agacctgaat gttgatgtca
     7681 ggcaactggc gtgccagata ccagcaagtt gaactggcgt ctaaggctat caccatccct
     7741 tcttcaatcc acgcgagcgc ctcgcgcgcg atatctgctt tatgcgcata atggcttttc
     7801 agcctgatgt gaaagggatc gccactgtct tgattttgac ggtggatata tttagcgcgt
     7861 ccatgattgc gcagaatttt accctgcgtc tgtaattcat tgagatcgcg acgaatggtt
     7921 tctttactga cctttagctg ttcagagaga gcttccgtgg tcaggctggt atggttcagc
     7981 agcaggtcga ctatcgcttg ctggcgtgcc gctttcatat ttttcacccc atcattcgat
     8041 aaccgcccgc gtcatgcggg cgtaccggta tgagattacg gtgttacccc tttttttaaa
     8101 agaatattcc cgtacttcgc tcgttcgcct gtgataacga tcgcaaaggc tttttgcgcc
     8161 cgttcataaa acgcaaaacg attgatgcgg atgatgtcag gacacggggc ttgtagtgaa
     8221 agcgcattac ggtaacgtcg ttctacttca ggatcgagag tgtcaccttc taccgctgcc
     8281 atcatcacca gcggcggtgc ataactgtcc agttcaaata acgggataat cgcctggagc
     8341 aagtcgctca ccaacaggcc atcagcgcgg atcacctgcg gtcccatcga atgggcggga
     8401 aagtgagcat cggaaaaaat aatttcatct ccatgtccca tctctgccag cactttcaat
     8461 agttcgggag aaattaacgg cgaaattgtt ttcagcattt cacacttcct ctataaattc
     8521 aggttcagtt tgcgggtaga aataacggta ctgataatga atctgcgcgc gggcttcttc
     8581 cgggctgtta aattccccta tgccatacca accgaacagc gcagctcctg cgacggtcgt
     8641 ttcggcgtcg tcgagaactt ttaccggaat atcaagcata ttggctttaa tctgattcca
     8701 caatgtgttg cgacttcctc cgccgactaa caataattca gaggctttaa agtgcccgat
     8761 tttttccagc atctgtagat tgcgctgtaa ttgcgcggtt aacccttcca gcgccgcgcg
     8821 atagaaatgc ccccgcgtgg tattaagcgt cactccttgc cagccagcgt tctgacacga
     8881 caataaatca cactgcattt ttacgccatc cgcgccaggc gcgatcagac gagcctcttc
     8941 aatcaacatt tgccagggtg tttcagccgt ccagaacagt tttctcaccc attccagcac
     9001 gccggatgcc agccattgca tacctgggtt atacaaccct gcctggctat ccagttcgca
     9061 ggtggaaccg gcgtactgac ttaacagcga agtatcaacc tgggcgctac ggaccattaa
     9121 aatttcccat gtaccggaag agagcacggg ttcattttgg tcggcaccag cgccaaaaag
     9181 ggcgaactgg gtatcgtgac ctgcggaaat caccggtatg ccaacgggta agccgagcat
     9241 tgctgcggcg ctgttctgta gcgtaccaat ctgttcaccc gcttccacca gacgagggaa
     9301 gagtcggcgt ggaataccgg tggcttgtaa aatttgcgga ctgaaatcgc gttgctggat
     9361 atccagcatc tggctggttc cggccatcgt gatatcagta gtgaattcgc cggttaaacg
     9421 gtggttaatc agcgacgaaa taaagagcca ggcgtgcgcg cgttccagca gttgtggatg
     9481 attttctttc aaccacacca acttatataa cgtattgaaa ctaaaggctc cgacgccaga
     9541 aatagcctgc aaccgctgtg cggagatcaa ctgttcaata ttgtccataa ccgctgctgt
     9601 tcgcggacat ttccagctaa tgatcggata gagcagattg ccttgcttat ctaccagagc
     9661 gccatccaca ccaaaggtgg tgacggcgat accgcggatg tggcattcag tcagttcact
     9721 attgatttgc cgacagcaat cagcaaagcg ttgcaaaatg gcgtctaaag accactggtg
     9781 ccaggtgttg ttttccatcg cgatatcgct ggcattaggc gttgaggcgc gggcaacaat
     9841 ttttccctgc cgattaaccg cgatggccct gacattggtc gcgccacagt cgagtaccag
     9901 gataacttct tgtttcataa tcgctcctgc aatatagccg gataacattg cttatccggc
     9961 taaccactct tgcttgttca ggcccggtag ccggagcgac cgggcatcac atcagggagt
    10021 aatgtattaa cgcttgtaca acggaccgta gttctggcaa gcgcggtaat cctggccttc
    10081 aatatccatg ccgtgcgcag cccaggcaga aggacgatac actttggtct cttcaacgtt
    10141 gtgcatacat accgggatac gcagcatgga ggcgagagtg ataaagtctg cgccaacgtg
    10201 gccgatggtc agaaccccat ggttagcgcc ccagttcgcc attaccgagt acacatccgt
    10261 aaacggccct ttaccggtga ggcgcggtgc aaaccaggtg gttggccagg ttgagttggt
    10321 gcgtttgttg aggatgtcat gcacatcctt cggcaattcc acgctccagc cttccgcgat
    10381 ttgcagtacc ggtcccaggc ctttgatgat gttgacacga gtcatggtga acgggacgcc
    10441 gccttcggta aggaagcggg aagagtaacc gccgccacgg aagtattcgt ggatcgccgg
    10501 gcaccattcg gtagcggcga ggcaagcgtc agcctcttgc tgagagattt cccagtgtgg
    10561 cttcatcgtc gggttacctt cgctgtcgcg ttgtttacag gaaccgtcca gcgcagcaga
    10621 acctgagttg atcaaatgga tgatgccgtg ttctgccagt ccatcgagtt tatgccccgt
    10681 tacacgctca attgcttctg gtgaccagta ggtacgcaca tcggcaaata cctgagcggt
    10741 gccggtgagc tggtgaccca ttagcattgc cacgccgtta agactgtcgt tttcggtcgc
    10801 cacgacaaag ggttcgcgca cgccattcca gtcaaatgaa ctgttgagga tcgcttcggc
    10861 ggtgtcacca ttgggatatt gatcggtcca gtgacgttgc ccctggaagc ccgcagcgat
    10921 ggcgttgtag ccaagtgatt cttccacgcg accaatatcg gccagtttgc tgttgccttg
    10981 catcatgtcg cggatacaca tcgccatcag taaactttcg cgcagaactg cgcggctttg
    11041 ctcggcatta cgttgatact gtttgttatt ttcatcttcg ccatagcgga agtttttatc
    11101 agcccaggcc agcgccattt ccagttcggc ttcgtcgtaa atctgctgat cgatacggcg
    11161 acgcagttcg gtcatatcca ccgcctggac tttcattccc agccaggatt caaagaagtt
    11221 gtgatcaaca atagaaccgg cgatacccat cgaaacgccg cccagcgaca gatagctttt
    11281 acctttcatg ctggcgacgg ccaaaccggc gcgggcaaag cgcagcagtt tttcttcaac
    11341 atcggcagga atcgatgtgt catcggcatc ctgaacgtca tgaccgtaaa tggagaatgc
    11401 cgggatgcct ttctggctgt gagctgccag agccgctgcc aggtaaacag cgccgggacg
    11461 ttcagtgccg ttaaagcccc aaatggcttt cgggcgggtt ggatccatgt cgatggtttc
    11521 actgccatag caccagcaag gcgttaccgt aatggtgagg cctacattct gactgctgaa
    11581 tttttcttcg caagcagcgg cttcagccat acccgcgata caggtatcgg aaatgacaca
    11641 ctcgacggca gctccgcagg catggcgcag tttctcggtc agcagtgcgg ccgtagcttt
    11701 cgccatattc attgtttgtt cttcaagcga ctcacgaaca cccatgcgac gaccgtcaat
    11761 aaccgggcgg ataccaattt tcggtaagct gatttttttc attacagatt cctcacttta
    11821 ttcggaaaat atgttcagtt agttgccgtt tgagaacgga aacgggcaaa gataaagatg
    11881 accgcgaagc agagtgcggg gatcagttca gcagtgggga tgttgcccgc cgcgtcactg
    11941 acaaaaccca tgaccggagt gacaataccg ccgccaataa tggtcataac gatgaaggac
    12001 gaaccgtatt tggtgtcctg gccgagattc ttaatgccca gcgagaagat tgttgggtac
    12061 tgaatcgaca taaaggcgct gcataaagtc agggctatta agcccacatg accgccagcg
    12121 aaggctgaga tcaggcacag tgccatagcg attaatgcgt aggcggccag gactttgtgt
    12181 ggtgcgaagc gactgatgag ccaggtaccg gtgaaacgac caataaagaa gcacaccatg
    12241 gttccggtta aatagttagc ggcaaagcct gcagtcatac ctggaatttc ttctacagcg
    12301 tagcgaatca aatagctcca gcaggccgtt tgtgcgccga catagcagaa ttgcgctaat
    12361 accgcccagc gccagtgacg aatacgcgcc agacgagaaa gcgatgcgga gaacgatcct
    12421 tgtttggcgt cactgtgatt atcactctgc aatgccggga atttcgtcag catgatcagc
    12481 agggcgacca gtaacacgat agccacgatg atcatataag gtgtctgtac cgataatacc
    12541 aggctgtgtt tatacgcact caattgctct ggagacattt tatcgagaac gtcttgcgat
    12601 tgatgtggca cgttagacaa aataagactt tgcccaaaga caaccgcgat aattgcgcca
    12661 aacgagttaa atgtttgcgc aagatttaag cggaagtgac cactgctttc tggccctaat
    12721 accgtaacaa aagggtttgc ggcagtttcc agacaaccta atccggctgc aataataaat
    12781 aggccaacta aaaataaggt gtagttcatt atttctgcag cgggccagaa taatgcagcg
    12841 cccaaggcat ataaaaataa tccggtaata atccctgctt tataactgag ttttttcatc
    12901 aatatcccag cagggattgg gataatgaaa taaccaaagt aaaaggccga ttggatcagg
    12961 ccagcctgga aatttgtcag cgtaaaagct tgctggaatt gaggtaataa aatgtcgtta
    13021 aggttattgg ctaccgccca aagaaaaaac agtgagcaca gcagcgcgaa tggaataatg
    13081 taacttctgc tttgccctgc atctttatct accgcacggt aactctgcgt ttgtattgat
    13141 gtgtttccca tagcatcctc ttaggttcag aagcttaagc acgtaatcgg ttatgttttc
    13201 aaaagtgcaa aaccagcgag ttcgggcttc atatcagtga atatttgcct gtctgaaatg
    13261 aactcatgga ttcattttca aataaaaaac gctctgtaat atgacggcgg tcacacttat
    13321 tagctggaag gtgactttat gtgactacca tcactttaat aatcgaaatt tcatgcccgt
    13381 ttcagaaaat aaatcataaa aaacacaaac cagatgaccg tattaattgt ttgtgccgaa
    13441 aacgggcatt cctggctggg agaagtagaa aaatgaccgt tttgataaat atttttaaga
    13501 gcggtcattt ttaatggggt tataaaaaga gaacttgcag ttatttactg tgattagttg
    13561 aaccaggtca caaaatataa gcttgtgaat ttctttcttg ttaatcagga agaagatggt
    13621 gggtaattaa acggctaatt caatagtgtg aaaggaacaa cattattgcc ctgttttgaa
    13681 tcagagagag gtagctaatg gaacgaaata aacttgctcg tcagattatt gacacttgcc
    13741 tggaaatgac ccgcctggga ctgaaccagg ggaccgcggg gaacgtcagt gtacgttatc
    13801 aggatgggat gctgattacg cctacaggta ttccatatga aaaactgacg gagtcgcata
    13861 ttgtctttat tgatggcaac ggtaaacatg aggaaggaaa gctcccctca agcgaatggc
    13921 gtttccatat ggcggcctat caaagcagac cggatgccaa cgcggttgtt cacaatcatg
    13981 ccgttcattg cacagcagtt tccattctta accgaccgat ccccgctatt cactacatga
    14041 ttgcggcggc tggcggtaat tctattcctt gcgcgcctta tgcgaccttt ggaacacgcg
    14101 aactttctga acatgttgcg ctggctctca aaaatcgtaa ggcaactttg ttacaacatc
    14161 atgggcttat cgcttgtgag gtgaatctgg aaaaagcgtt atggctggcg catgaagttg
    14221 aagtgctggc gcaactttac ctgacgaccc tggcgattac ggacccggtg ccagtgctga
    14281 gcgatgaaga aattgccgta gtgctggaga aattcaaaac ctatgggtta cggattgaag
    14341 agtaatttcg taaagcaaca aggagaagga tgatggctaa cagaatgatt ctgaacgaaa
    14401 cggcatggtt tggtcggggc gctgttgggg ctttaaccga tgaggtgaaa cgccgtggtt
    14461 atcagaaggc gctgatcgtc accgataaaa cgctggtgca atgcggcgtg gtggcgaaag
    14521 tgacccataa gatggatgct gcagggctgg catgggcgat ttacgacggc gtagtaccca
    14581 acccaacaat tactgtcgtc aaagaagggc tcggtgtatt ccagaatagc ggcgcggatt
    14641 acctgatcgc tattggtggt ggttctccac aggatacttg taaagcgatt ggcattatca
    14701 gcaacaaccc ggagtttgcc gatgtgcgta gcctggaagg gctttccccg accaataaac
    14761 ccagtgtacc gattctggca atccccacca cagcaggcac tgcggcagaa gtgaccatta
    14821 actacgtgat cactgacgaa gaaaaacggc gcaagtttgt ttgcgttgat ccgcatgata
    14881 tcccgcaggt ggcgtttatt gacgctgaca tgatggatgg tatgcctcca gcgctgaaag
    14941 ctgcgacggg tgtcgatgcg ctcactcatg cgattgaggg gtatattacc cgtggcgcgt
    15001 gggcgctaac tgatgcgctg cacattaaag cgattgaaat cattgctggg gcgctgcgag
    15061 gatcggttgc tggtgataag gatgccggag aagaaatggc gctcgggcag tatgttgcgg
    15121 gtatgggctt ctcgaatgtg gggttagggt tggtgcatgg tatggcgcat ccactgggcg
    15181 cgttttacaa cactccacac ggtgttgcga acgccatcct gttaccgcat gtcatgcgct
    15241 ataacgctga ctttaccggt gagaagtacc gcgatatcgc gcgcgttatg ggcgtgaaag
    15301 tggaaggtat gagcctggaa gaggcgcgta atgccgctgt tgaagcggtg tttgctctca
    15361 accgtgatgt cggtattccg ccacatttgc gtgatgttgg tgtacgcaag gaagacattc
    15421 cggcactggc tcaggcggca ctggatgatg tttgtaccgg tggcaacccg cgtgaagcaa
    15481 cgcttgagga tattgtagag ctttaccata ccgcctggta aatgcgctga tgtgataatg
    15541 ccggatacga cgtttgcggc gtatccggct cgccgttacc gtaccaatcg caattgctgt
    15601 aaattgccgt cgatatgcaa atcggtttgt aagcgggcaa tatcgcggca cagaaacgcc
    15661 atctctttat gggtttctaa ttttttgcgc cacttttcgg caaccgcatc cagattctca
    15721 tatatccctt ccagactctg aaactcgacc agcagctgcg tggcgctttt tggtccgatt
    15781 cccgcaacac ccggtacctt tgaactgctg atccccgcaa gtccccagta atcgggcaac
    15841 tgctgcggtt gaacgccaaa ttctttatcg ataaatggcg catccagcca acgtttctgg
    15901 aagtaatcac gaatacgtaa tgtcggtgaa agtaactgac agtagccttt atctgtcgaa
    15961 acaatcgttg cctgatgccc ggcctgtgtc actttgaccg ccagcgtggc ggctaagtca
    16021 tcggcttcgt tgccgctggt tgaccagcac gggacgccgc gttgctcaaa ggcggcgcgt
    16081 aatgcaggca tctcgtcgtg caactcttcc ggcattggcg gtcgacccgc tttgtaatct
    16141 ggtaaacgct gatgacgcca gccgctactg cggttttcat catcaaaaac ggcgaccgcg
    16201 tgggttggct ggctgtgcat aatgagctga tcgagcgcat gctggcaggt ttcgacacag
    16261 ggcgacccct gaacggcatg aatgcgacga ataagattca gtgcatcgac aataagcaaa
    16321 tgaacagcca cgataacccc cttaaagatc aggcgataag ggtaacattc gatactggaa
    16381 caggctacgg ctggatgaga aatcgggaag aggcctcgca aaacgaggcc tctggagagc
    16441 gattaatcgc aggcaacgat cttcattgcc aggccgccgc gagaagtttc gcggtacttg
    16501 gcgttcatat ctttacctgt ttcgtacatg gtttcgataa ctttatcgag gcagacgcgc
    16561 ggctcgctgg tacggcgtag cgccatacgt gcggcgttca ccgcttttac tgccgcaatg
    16621 gcgttacgct cgatgcatgg cacctgtacc tgtccggcga ccgggtcaca cgtcagaccg
    16681 aggttgtgct ccatggcgat ttccgccgcg atgcacacct gcgccgggct tgcgcctaat
    16741 agttctgcca gaccagccgc cgccattgag cacgccacgc caacttcacc ctggcaaccc
    16801 acttcagcac cagaaatcga cgcgttcatc ttataaagag aaccaatggc gctggctacc
    16861 agcaggtaac gagccagtga gttagcgttc acttcgcgga taaacttatc gtagtacgcc
    16921 agcacagccg ggataatccc gcacgcaccg ttagtcggcg cagtcaccac gcgaccgcca
    16981 gcggcgttct cttcgttcac tgccagtgca aacatgttga tccagtcaac aactgccatc
    17041 gggtcagtgg tggttttatc ctggctgacc agcatccggc gtagtgccgc agcacggcgt
    17101 ggaacgcgca gtttgccagg caacacgcct tcggtggaaa taccgcgctc aataccgccg
    17161 cgcatgactt cccagacgtt cgccaggtgc tgttccagct cttctttgct gtgcagcgcc
    17221 agctcgtttt tcatcatcag gccagagagt gacagcccgg tttcctgaca atgtttttgc
    17281 agatcggctg ctgaactgta cggataagga acttcaaccg gtgcgctatc ctgctggcca
    17341 aaatgctctt catcaacgat aaagccaccg ccaatagagt agtaagtctg actgtaaacg
    17401 actttatcac ccgccagcgc ggtaatgcgc ataccgtttt catgcagaga aaggttgtcg
    17461 gcatggaagt tcatgcactg atcaaccggg aactccactt catgctgacc gtttgccagc
    17521 atcaggcgac catgagtatt cacatcctga ataaaaccgg ggatggaatc gatatccacg
    17581 gtatccggca ggttacccgc caggcccata ataatggcga tatcagtgtg gtggccttta
    17641 ccagtcagag agagcgagcc gtacacgtca accaccacgc gggtcacgtc tttaagcagg
    17701 ttacgggcaa tcagatcgtc ggtaaattgt ttacccgctt tcattggtcc aacggtatga
    17761 gaactggaag ggccaatgcc gattttgaaa atatcgaata cgctaatcat aggaaataca
    17821 tcgcgttaaa acggaggaag cgccgcccga aagcggcgcg aaaggactta gctgaacaga
    17881 gagtagaaga ttgcggagat tgcaatcaga cccatcacga caacgaatac gttgctgatg
    17941 tgaccgctgt acttacgcat tgccggtact ttctgaattg cgtacatcgg catcaggaac
    18001 aggatcatcg cgatgattgg gccgcccagg gtttcaatca tacccaggat gctcgggttc
    18061 agggtggcaa caatccaggt cgttaccagc atgaacagcg cagtgatacg gttcagcttg
    18121 ttgatttcga tagacttacc tttaccacgc agagatttaa tcaccatacc gttgaagcct
    18181 tcacgtgcgc ccaggtagtg gccgaggaag gatttggtga tagcgataat cgcgataatc
    18241 ggagccatcc acgcgataac cggtgcgtta aagtggttag ccaggtaaga cagaatcgag
    18301 atgttctgct ctttagccgc agccaggtct gccggagtca ggctcagtac acagctgaat
    18361 acgaagaaca ttacggtcag caccatcatg atgtgtgcga atgccaggat cttcgagcat
    18421 ttctgttctg ccatatcgcc gtactcttca cgcttcgcaa cggcgaaaga agagatgatc
    18481 ggagagtggt tgaacgagaa caccattacc ggaattgcca gccacagggt catccacaga
    18541 ccgtttccgg ttgcagatgc agtgtccaga gacagcgttt ccagtgctgc gccgttccac
    18601 tgcgggatca ggtacagagc cagcagcatc aatacgccaa caaacgggaa taccagaata
    18661 ctcatcgctt taacgatcat ctgctcaccg aagcgaacga tggtcatcat acccacgatc
    18721 aggatcagcg acagaatcgc acgcggcggt ggtgtcatac ccagctggtg agacatgaag
    18781 ctttcaactg tattggtgat tgctacgcta taaaccagca ggatcgggta gatagcgaag
    18841 aaatagagca gggtaatcag tttacctgcg ccaataccaa agtgttcttc tacaacctcg
    18901 gtgatgtctt cgcccgggtt tttaccagac agtacgaagc gagtcaggcc gcggtgtgca
    18961 aaaaatgtca tcgggaacgc aaggatagcc atgatgatca gcgggatcat accgccaaca
    19021 ccggcgttga ttggcaggaa cagcacgccc gcgccgattg ccgtgccgta aaggcccagc
    19081 atccacatgg tgtctgtctt gcgccaggca ctacgagagt ctttcgacgc aatcgtgctg
    19141 gtttgagtcg tttccatcta tttctcctgg aggaatgcaa ataattcagc ttttagccag
    19201 ttcagcccgg gaaggtgcct gaccgtaaaa atgaaatctg tctatcggcg atggcttaat
    19261 catttaccgc cgtaataatt tgagggcgga aagatacaca caagttatgt atctatcagt
    19321 gatcttgatc tcaaatacaa taatctactt ggaagataga ttatcccaac gctttactct
    19381 gtaaatcaaa agtaaaagag aatttaaggg caatatttgc ccattaaacg acatctgact
    19441 tcttccgggg cgcgcaatat atgtaggttg aactgaaaaa atcaatactt ttagttaatc
    19501 atggcgataa tttctttttc atagcgataa aatacggttt atgaaaatag ggaaacgttt
    19561 gcgttgcaac ataaattgta tgacatatta actgtgatga tatttgtcgc gtgagggtgg
    19621 taagaaacat tcatataaca aatgaaatga gttgatgagt aagtgaccaa aaacgagaaa
    19681 acccggcaac aaacgtcacc gggggagggg agattacgtg cagatttcgt agcaagggat
    19741 gtaggctgag cctggcaact tcatacgatg ctgggcaaca aaaccctgta gcaggtcgtc
    19801 catacgacgc ataatctctt tatcgccgtt gattttgtaa ggaccaaact cttcaatggc
    19861 gcgaataccg acttctttta cgttacccgc cacaataccg gagaacgcac ggcgcaggtc
    19921 ggcagctagc acttcaacgg gttgatccgg gtaaagcttc agattagcca tattctcgtg
    19981 agacggctca aacggcattt gcaaatctgg cgcaatgcgc attgaccagt taaagctgta
    20041 ggcatcgcct gtgtcgcggc gattttcttt caccagcggc atcgattttt tcatctgacg
    20101 agcgacttca gcggcgtcat caatgatgat gcggtaatgg cggcgcgcgt tttcacccag
    20161 cgtatgcacg acaaactcgt ccagtacgcg gaagtagtcg gcgctctctt tcgggccggt
    20221 gaggatcaat ggtaaaacct gatctttgtt ggccgggttc attaaaattc ccagcaaata
    20281 cagcaactct tctgccgtac ccacaccgcc agggaagata atgataccgt gagcgatacg
    20341 gacaaacgct tccagacgtt tttcgatatc tggcatgatg atcaattcgt tgaccagcgg
    20401 gttaggcggt tcagcggcga taatcgacgg ctctgtcata ccaataaaac gactgtcttt
    20461 gtaacgctgc tgcgcgtgtc cgaccgcagc acctttcatc ggcgcttcca tcgctcccgg
    20521 accacagccg gtgcagatat tcagctcacg caggcccagc tggtttccga cgcgacgggc
    20581 atacaaatac tcgttttcgt taattgagtg accgccccag cagacaacca tatttggcgc
    20641 ttcacccaca tgcagcgcgc gagcgttacg caagatggaa aagaccaggt tagtgatgtg
    20701 gacggagttg tcgagattca ggttgggaaa acgaacggtg ttatggattt gcccgtaaac
    20761 gaagagaatg tcacgcagga ccgcgaacaa gttggcctgc aaagcgcgaa taattcgccc
    20821 atcgacaaaa gcctcttccg ggggattaat cagttccagc tttacgccgc gttcacggcg
    20881 caagacgtta atatcgaaat tttcaaaacg agacagcaat tctttgctgt tatcggtcaa
    20941 actaccggag ttcagtacgg caagtgaaca gttgcgaaac agttgataga ggtcgctgct
    21001 ggcggtgcgt ttaagcatat ccacttccag ctgcgacaac atatccatgg agccaagcgg
    21061 gctaatatgt gtaatcaaga aaaactcctt atgggacgaa tatcgtcatt ccctttgatt
    21121 acaatagccc tgcctgatga cctttcacaa ccttctgcgt gaatccagca cgcaaaattg
    21181 agaaaaaatt tattgccgaa ccagtcttgt ggtcgatggg acaaaatcgc tattactgcg
    21241 ccacgggtta atgtccagac cgccacgacg ggtataacgt gcgtaaacgc tcaatttctc
    21301 tggctggcag aagcgtaaca ggtcattaaa gatgcgttcc acgcactgtt cgtggaactc
    21361 gttgtgatga cggaatgaga ccaggtaacg cagcagtttt tctctgtcga tttggcgtcc
    21421 acgatactga atttggatcg atccccaatc tggttgatgg gtgatcaggc agtttgattt
    21481 cagcaggtgg ctgacaagcg tctcttccac tactttttca ccactggtgg cattctccag
    21541 atagtcagta gtgaattcat agttatcgat agtgatatac tggtcatcaa tgcaagtgcc
    21601 attgaaatga cctatcggct ggccttccag ttcatcaaga cgatataacg ccacgctaac
    21661 cttaccctga gcgcaagtgc ttaagtcgcg ctccagcgtc tggcgcacct catcccagtt
    21721 attaaaacgc gtctggttaa aactgttgag ataaagctta aaactcttcg actcaatcag
    21781 atttacgctg gtgtaatcaa gttcaacatg accgacagcg acctgcggca aacctttcgc
    21841 attcagccag gaaagttcat acagcgtcca gatatccgta ccgtgaaaag gcaggttatc
    21901 cgctttcaga cccagcgggt cgcgattcag gctgcgtgga acgccttgca gtaggctggc
    21961 gtcataggta tcccggtaat cggttgattt tccaagagtc aggcccgcaa gtgcctgatg
    22021 gtttgcataa gaagacatgt ttcaccgtca taaaccagat aaactaaacg acaagtgtat
    22081 ctggtttaag tgaagagaga aattagtgga cgatttgacc gcacaagccc tgaaagattt
    22141 tactgcacgc tactgcgatg cgtggcatga agagcataaa agctggccgt taagtgagga
    22201 actgtacggt gttccttcac cgtgcattat ttcaactacc gaagatgccg tatactggca
    22261 accccagccg tttaccgggg aacaaaatgt aaacgcggtt gaacgcgctt ttgatattgt
    22321 gatacaaccc acaattcata ccttttatac gactcagttt gccggggata tgcacgcgca
    22381 gtttggcgat atcaaactga cattgctgca aacctggagt gaagacgact tccgtcgggt
    22441 gcaggaaaat ctgattggtc atctggtgac tcagaaaagg ctaaaactgc cgcccacgct
    22501 atttatcgcg acgctggaag aagagttaga ggtcatctcg gtgtgtaatc tctctggtga
    22561 agtgtgcaaa gagacgctgg gaacacgtaa acggacgcat ttagcctcaa atcttgcgga
    22621 attccttaat caacttaagc ctcttctgta atccaaatac cccatctggt tgtgagagat
    22681 ctcttacaga ctctgtagga gatcgccagg aaataagcga atacttaaaa agataagaat
    22741 cgttattttc atttaaaatc aaaatgttga ttgttaattc ttaactttca tatgaaattt
    22801 tccttaaggc attgtctgta agcgccttgt aagacttcgc gaaaaagacg attctatctt
    22861 cgtcgacagg gagtcagaca acgaagtgaa catcaggatg atgacacttc tgcaggacac
    22921 accaggatgg tgtttcaggg aaaggcttct ggatgaagcg aagaggatga cgcaggacgc
    22981 gttaaaggac acctccagga tggagaatga gaaccggtca ggatgattcg gtgggtcagg
    23041 aaggccaggg acacttcagg atgaagtatc acatcggggt ggtgtgagca ggaagcaata
    23101 gttcaggatg aacgattggc cgcaaggcca gaggaaaagt tgtcaaggat gagcagggag
    23161 caacaaaagt agctggaatg ctgcgaaacg aaccgggagc gctgtgaata cagtgctccc
    23221 tttttttatt cctgctatcc ttcgcggcag tttttcttat tgaggttgct ttatgaccac
    23281 tcatgaccgc gttcgtctcc agttgcaggc gcttgaagcg ttactgcgtg aacatcagca
    23341 ctggcgaaac gatgaacccc agccgcatca atttaatagt acccaaccgt tctttatgga
    23401 caccatggaa ccactggagt ggttgcagtg ggtgctgatc ccgcgaatgc acgatttgtt
    23461 aaataacaac cagccgttac cgggcgcttt tgctgttgca ccctattatg aaatggcgct
    23521 ggcgacggac catccgcaac gcgcgcttat tctggctgag ctggaaaaac tggatgctct
    23581 ttttgcggat gatgcgagct aatgctggaa atactctatc aggatgaatg gctggttgcg
    23641 gtaaataaac cctccggctg gctggttcac cgcagctggc tggatcgcga cgagaaagta
    23701 gtggtcatgc aaaccgtgcg tgaccagata ggccagcatg tttttactgc tcatcgtctg
    23761 gaccgaccca cttctggtgt gttgttgatg ggattatcca gcgaggccgg acggctgctg
    23821 gcacaacagt ttgaacagca ccaaatccag aaacgttacc atgcgattgt gcgcggctgg
    23881 ttgatggaag aagcggtgct ggattatcca ctggtggaag aactggacaa aatcgctgat
    23941 aaatttgccc gcgaagataa aggcccgcag ccagcagtga cgcattatcg cggtctggcg
    24001 accgtagaaa tgccggtagc gaccggacgt tacccgacca cgcgctacgg cctggtggaa
    24061 ctggagccga aaaccggacg caaacatcag cttcgccgac atcttgccca tttgcgtcat
    24121 ccgattattg gcgatagcaa acatggcgat ttacgccaga atcgcagcgg tgctgagcat
    24181 tttggcctcc agcggttaat gctacatgcc agtcagttgt cattgacgca tccttttact
    24241 ggcgagccgc tgactattca cgcgggcctg gacgacacct ggatgcaggc gttatcacaa
    24301 tttggctggc gcggtctgct tcctgaaaat gaaagggttg agttcagtgc gccatcgggt
    24361 caggatggag agataagttc ctaatccagg gagaaaacgg tatggcggaa attggtattt
    24421 ttgtcggcac catgtacggg aattcactgt tagtggccga agaagcggaa gcgattctga
    24481 ccgcgcaggg ccacaaagca acggtatttg aagatcctga attaagcgac tggctgccct
    24541 atcaggataa gtatgttctg gtggttacgt ccacgaccgg gcagggcgac cttcctgata
    24601 gcattgtgcc actctttcag ggaatcaaag atagtctggg cttccagccg aatctgcgtt
    24661 atggcgtgat tgcgctcggc gacagtagtt atgtgaattt ctgcaatggc ggcaaacagt
    24721 tcgatgcctt gttgcaggaa cagagcgctc agcgggttgg cgaaatgctg ttgattgacg
    24781 ccagtgagaa cccggaaccg gaaacggaat caaatccgtg ggtcgaacat tggggcacgc
    24841 tgttgtcctg atatgttcag cgagcggtaa atgccgtttt agcagtgctg aatcgaatct
    24901 tttcaggcaa atgccagtaa aaactgcttc atagtgcgga tttttactgg cgtttgcctg
    24961 gagtcaagcg atccatttca tactcttctt tatttcttcg ttttaaccct tcctttcttg
    25021 ttcttgtttt catttccgtg aagtggattc caccgtccag ggctaatgcc aaaatcgggc
    25081 ctcattgaac gcattaatgt tgtgttgttg cacggtgagc cgctatggcg cgctttttat
    25141 actgctattg ccagatataa acacgcgccg tattcggcga acgacctata aaaacggcaa
    25201 aaaacaccct acgtcacctc tgatttcctg gcgatgtcgc agtccagagt gagcgtggct
    25261 aacgcgaatt ttcaggagtg caacaatgag ttctttaagt caggctgcga gcagtgtgga
    25321 aaaacgcaca aatgctcgtt actggatagt ggtgatgttg tttatcgtca catccttcaa
    25381 ctacggcgac cgcgctacgc tctctatcgc cggttcggaa atggccaaag atatcggcct
    25441 tgatcctgtg ggaatgggct atgtgttctc tgctttctca tgggcttatg ttatcgggca
    25501 gatccctggt ggctggttgc tggaccgttt tggttcaaaa cgcgtctact tctggtcgat
    25561 ctttatctgg tcgatgttta ccttgctgca aggcttcgtc gatatcttta gtggattcgg
    25621 cattatcgtt gccctgttta cgctgcgctt cctggtcggg cttgctgaag cgccatcttt
    25681 ccccggcaac agtcgcattg ttgcggcctg gtttccggcg caggaaaggg gaacggcggt
    25741 gtcgattttt aactccgccc aatacttcgc aacggttatc ttcgcgccga ttatgggctg
    25801 gctgacgcat gaagtgggct ggtcacacgt cttcttcttt atgggcggtc tgggcattgt
    25861 catcagcttt atctggttga aagtcatcca cgagccaaat caacatccgg gcgtaaatca
    25921 gaaagagctg gagtacatcg ccgcgggtgg cgcgctgatc aatatggatc agcaaaacac
    25981 caaagttaaa gtaccgttca gcgtgaagtg ggggcagatc aaacagctgc tcgggtcacg
    26041 gatgatgatc ggcgtttata tcggtcagta ctgtatcaac gccctgactt acttctttat
    26101 tacctggttc ccggtttatc tggtgcaggc gcgtgggatg tcgattctga aagcgggctt
    26161 tgtggcttcc gttccggcgg tttgcggttt tatcggcggt gtgctgggtg ggattatttc
    26221 cgactggatg atgcgccgca cgggatcgct gaacattgcg cgtaaaacac cgatcgtaat
    26281 gggcatgttg ctgtcgatgg tgatggtgtt ctgcaactac gtcaacgttg agtggatgat
    26341 catcggcttt atggcgctgg ccttcttcgg taagggcatc ggggcgctgg gttgggcagt
    26401 aatggcagat accgcgccaa aagagatcag cggtctttcc ggtggcctgt tcaacatgtt
    26461 cggtaacatt tctggcatcg tcacgccaat cgcaattggt tatatcgttg gcacgactgg
    26521 ctcgtttaat ggggcgctga tttatgttgg tgttcatgcc ttaatcgcgg tactgagcta
    26581 cctggtgctg gtgggcgata tcaagcgtat cgagttgaaa cctgttgcgg ggcaataaga
    26641 tggcgacaca atccagtcct gttattactg atatgaaagt cattccggtg gccgggcatg
    26701 acagcatgtt gcttaatatt ggtggggcac ataacgcata tttcacccgc aatattgtgg
    26761 tactcaccga taacgccggg cataccggca ttggtgaagc gccgggcgga gatgtgattt
    26821 atcagacgct tgtcgatgct attccgatgg ttctgggcca ggaagttgcg cgactgaata
    26881 aagtggtcca gcaggtgcat aaaggtaatc aggccgccga ttttgatacc ttcggtaaag
    26941 gtgcctggac ctttgaatta cgcgttaacg ccgtggcggc gctggaagcc gctttgcttg
    27001 acctgctagg taaggcgctg aatgttccgg tctgcgaact gttagggcca ggcaagcaac
    27061 gcgaggctat taccgtcctc ggttatctgt tttatatcgg tgatcggacc aaaaccgatc
    27121 ttccttatgt ggaaaatacg ccgggcaacc atgagtggta tcagttgcgg catcagaaag
    27181 caatgaacag cgaagccgtt gtgcgtctgg cggaagcctc acaggatcgc tacggcttta
    27241 aagatttcaa acttaagggc ggcgtgttac ctggcgagca agaaatcgac actgttcgtg
    27301 cattgaagaa acgcttcccg gatgcgcgga ttaccgttga tcccaacggt gcatggctgc
    27361 ttgatgaagc catttcttta tgcaaagggc tgaatgatgt tcttacctat gccgaagatc
    27421 catgcggcgc agaacagggc ttctccggac gtgaagtgat ggcggaattt cgacgggcga
    27481 ccggcttgcc cgtcgcgact aacatgatcg ccaccaactg gcgcgaaatg ggtcatgcgg
    27541 tgatgctcaa tgcggtagat attccacttg ccgatccgca cttctggacg ctttccggtg
    27601 cagtccgtgt ggcgcagctt tgcgacgact gggggctgac ctggggctgc cattctaata
    27661 accatttcga tatctctctg gcgatgttta cccatgtggg cgcggcggca ccgggtaatc
    27721 ctaccgctat cgatacccac tggatttggc aggagggcga ttgtcgcctg acccaaaatc
    27781 cgctggagat taaaaacgga aaaattgccg ttcctgatgc gcccggtctg ggcgtggaac
    27841 tggactggga acaggtacaa aaggcacatg aggcctataa acgtctgcct ggcggtgcgc
    27901 gtaacgacgc aggtccgatg cagtacctga tccccggctg gacctttgac cgtaaacgtc
    27961 ccgttttcgg ccgtcattga ttctgaaaaa ggacataaat atgagttctc aatttacgac
    28021 gcctgttgtt actgaaatgc aggttatccc ggtggcgggt catgacagta tgctgatgaa
    28081 tctgagtggt gcacacgcac cgttctttac gcgtaatatt gtgattatca aagataattc
    28141 tggtcacact ggcgtagggg aaattcccgg cggcgagaaa atccgtaaaa cgctggaaga
    28201 tgcgattccg ctggtggtag gtaaaacgct gggtgaatac aaaaacgttc tgacgctggt
    28261 gcgtaatact tttgccgatc gtgatgctgg tgggcgcggt ttgcagacat ttgacctacg
    28321 taccactatt catgtagtta ccgggataga agcggcaatg ctggatctgc tggggcagca
    28381 tctgggggta aacgtggcat cgctgctggg cgatggtcaa cagcgtagcg aagtcgaaat
    28441 gctcggttat ctgttcttcg tcggtaatcg caaagccaca ccgctgccgt atcaaagcca
    28501 gccggatgac tcatgcgact ggtatcgcct gcgtcatgaa gaagcgatga cgccggatgc
    28561 ggtggtgcgc ctggcggaag cggcatatga aaaatatggc ttcaacgatt tcaaactgaa
    28621 gggcggtgta ctggccgggg aagaagaggc cgagtctatt gtggcactgg cgcaacgctt
    28681 cccgcaggcg cgtattacgc tcgatcctaa cggtgcctgg tcgctgaacg aagcgattaa
    28741 aatcggtaaa tacctgaaag gttcgctggc ttatgcagaa gatccgtgtg gtgcggagca
    28801 aggtttctcc gggcgtgaag tgatggcaga gttccgtcgc gcgacaggtc tgccgactgc
    28861 aaccaatatg atcgccaccg actggcggca aatgggccat acgctctccc tgcaatccgt
    28921 tgatatcccg ctggcggatc cgcatttctg gacaatgcaa ggttcggtac gtgtggcgca
    28981 aatgtgccat gaatttggcc tgacctgggg ttcacactct aacaaccact tcgatatttc
    29041 cctggcgatg tttacccatg ttgccgccgc tgcaccgggt aaaattactg ctattgatac
    29101 gcactggatt tggcaggaag gcaatcagcg cctgaccaaa gaaccgtttg agatcaaagg
    29161 cgggctggta caggtgccag aaaaaccggg gctgggtgta gaaatcgata tggatcaggt
    29221 gatgaaagcg cacgagctgt ataagaaaca cggacttggc gcgcgtgacg atgccatggg
    29281 aatgcagtac ctgatccctg gctggacgtt cgataacaag cgcccgtgca tggtgcgtta
    29341 agacagaaaa ccaaaaccgg ctctgtcagg agccggtttg agactgatgc taaacgcaaa
    29401 atagcctgtt gtgctatgta tcaggtctac atcatttttt aattcattga atttaattgg
    29461 ttt
//
LOCUS       DAOFQD010000058        28158 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.058, whole genome shotgun sequence.
ACCESSION   DAOFQD010000058 DAOFQD010000000
VERSION     DAOFQD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.058"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(171..1835)
                     /gene="glnS"
                     /locus_tag="QBF46_002539"
     CDS             complement(171..1835)
                     /gene="glnS"
                     /locus_tag="QBF46_002539"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDQ6674712.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2103..4049)
                     /gene="nagE"
                     /locus_tag="QBF46_002540"
     CDS             complement(2103..4049)
                     /gene="nagE"
                     /locus_tag="QBF46_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDQ6674713.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLGLIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4382..5182
                     /gene="nagB"
                     /locus_tag="QBF46_002541"
     CDS             4382..5182
                     /gene="nagB"
                     /locus_tag="QBF46_002541"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDQ6674714.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKDL"
     gene            5242..6390
                     /gene="nagA"
                     /locus_tag="QBF46_002542"
     CDS             5242..6390
                     /gene="nagA"
                     /locus_tag="QBF46_002542"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDQ6674715.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     KNADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6399..7619
                     /gene="nagC"
                     /locus_tag="QBF46_002543"
                     /gene_synonym="nagR"
     CDS             6399..7619
                     /gene="nagC"
                     /locus_tag="QBF46_002543"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDQ6674716.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7667..8419
                     /gene="nagD"
                     /locus_tag="QBF46_002544"
     CDS             7667..8419
                     /gene="nagD"
                     /locus_tag="QBF46_002544"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDQ6674717.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8816..10480
                     /gene="asnB"
                     /locus_tag="QBF46_002545"
     CDS             8816..10480
                     /gene="asnB"
                     /locus_tag="QBF46_002545"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDQ6674718.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10860..10936
                     /locus_tag="QBF46_002546"
     tRNA            10860..10936
                     /locus_tag="QBF46_002546"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10894..10896,aa:Met,seq:cat)
     gene            10945..11029
                     /locus_tag="QBF46_002547"
     tRNA            10945..11029
                     /locus_tag="QBF46_002547"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10979..10981,aa:Leu,seq:tag)
     gene            11053..11127
                     /locus_tag="QBF46_002548"
     tRNA            11053..11127
                     /locus_tag="QBF46_002548"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11085..11087,aa:Gln,seq:ttg)
     gene            11162..11236
                     /locus_tag="QBF46_002549"
     tRNA            11162..11236
                     /locus_tag="QBF46_002549"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11194..11196,aa:Gln,seq:ttg)
     gene            11252..11328
                     /locus_tag="QBF46_002550"
     tRNA            11252..11328
                     /locus_tag="QBF46_002550"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11286..11288,aa:Met,seq:cat)
     gene            11376..11450
                     /locus_tag="QBF46_002551"
     tRNA            11376..11450
                     /locus_tag="QBF46_002551"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11408..11410,aa:Gln,seq:ctg)
     gene            11488..11562
                     /locus_tag="QBF46_002552"
     tRNA            11488..11562
                     /locus_tag="QBF46_002552"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11520..11522,aa:Gln,seq:ctg)
     gene            complement(11716..12891)
                     /gene="ubiF"
                     /locus_tag="QBF46_002553"
     CDS             complement(11716..12891)
                     /gene="ubiF"
                     /locus_tag="QBF46_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDQ6674719.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHLYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAV
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            13037..14461
                     /gene="miaB"
                     /locus_tag="QBF46_002554"
     CDS             13037..14461
                     /gene="miaB"
                     /locus_tag="QBF46_002554"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDQ6674720.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRTLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14614..15654
                     /locus_tag="QBF46_002555"
     CDS             14614..15654
                     /locus_tag="QBF46_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDQ6674721.1"
                     /translation="MNIDTREITLEPTDNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15651..16118
                     /gene="ybeY"
                     /locus_tag="QBF46_002556"
     CDS             15651..16118
                     /gene="ybeY"
                     /locus_tag="QBF46_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDQ6674722.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16208..17086
                     /gene="corC"
                     /locus_tag="QBF46_002557"
     CDS             16208..17086
                     /gene="corC"
                     /locus_tag="QBF46_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDQ6674723.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            17111..18649
                     /gene="lnt"
                     /locus_tag="QBF46_002558"
     CDS             17111..18649
                     /gene="lnt"
                     /locus_tag="QBF46_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDQ6674724.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFGWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWILTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18776..20653)
                     /locus_tag="QBF46_002559"
     CDS             complement(18776..20653)
                     /locus_tag="QBF46_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDQ6674725.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGEPWRLLSSVFLHSSVSHLLMNMFAFLVVGAVAERILGKWRLLI
                     IWLFSGVFGGLISACYALRDSEQIVISVGASGAIMGIAGAAIATQLASGTGTHHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALKET
                     LPAPVSRQQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLLYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGKKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLIPGMVRGDIVRINTITHQKEDFPAGDARGTISAMRFRPENGDVIFAD
                     SQGISRIRVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINVGQ
                     IVGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTLSLDPQAVIRHPQYGNVACIPE
                     P"
     gene            21108..22016
                     /gene="gltI"
                     /locus_tag="QBF46_002560"
     CDS             21108..22016
                     /gene="gltI"
                     /locus_tag="QBF46_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDQ6674726.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            22186..22926
                     /gene="gltJ"
                     /locus_tag="QBF46_002561"
     CDS             22186..22926
                     /gene="gltJ"
                     /locus_tag="QBF46_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDQ6674727.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            22926..23600
                     /gene="gltK"
                     /locus_tag="QBF46_002562"
     CDS             22926..23600
                     /gene="gltK"
                     /locus_tag="QBF46_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDQ6674728.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23600..24325
                     /gene="gltL"
                     /locus_tag="QBF46_002563"
     CDS             23600..24325
                     /gene="gltL"
                     /locus_tag="QBF46_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDQ6674729.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24443..25378
                     /gene="rihA"
                     /locus_tag="QBF46_002564"
     CDS             24443..25378
                     /gene="rihA"
                     /locus_tag="QBF46_002564"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDQ6674730.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTSVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25462..27132
                     /gene="hscC"
                     /locus_tag="QBF46_002565"
     CDS             25462..27132
                     /gene="hscC"
                     /locus_tag="QBF46_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDQ6674731.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIQESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETP"
     gene            complement(27192..>28158)
                     /gene="djlC"
                     /locus_tag="QBF46_002566"
     CDS             complement(27192..>28158)
                     /gene="djlC"
                     /locus_tag="QBF46_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDQ6674732.1"
                     /translation="GDCFDFLSLLEYPIALQNQTVEYYFALERCCRYHPDYVTAFLAM
                     EGPWFIPDDAKLHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYC
                     GEGESLLAELCAYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEAQSMVDAQGQPL
                     KYVPGESARTRLLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPL
                     LDLYRVAEQVVLEAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVD
                     EPTPENHEEKNDEGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
BASE COUNT         6925 a         7330 c         7130 g         6773 t
ORIGIN      
        1 tgcggcgtga acgccttaac cggcctacaa aagcacgcaa actcaatata ttgcagagat
       61 catgtaggcc tgataagcgt agtgcatcag gcaatttagc gtttgaaact gttgtctgtt
      121 ccttgccgga tgcgacgtaa acgccccatc cggcatagcg aaacttaaaa ttactcgcct
      181 actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttccgccgta
      241 gaatggcggc tatcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca
      301 accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta
      361 atcaccgaca ggaaatcatc cgcagcacca gggttcggca cgctgaacag acggtcgtac
      421 agacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact
      481 ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg
      541 gtgatattgc cttcggcatc tttctccacg cgttctgcct taatgacgta agcattacgc
      601 agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc ttcgcggaaa
      661 tcggcgcgat cgatccaaat ctcaccgcta aacggcacct ggcggctacc catttccggt
      721 ttgttcggat ggttcggcat ggtaaccatt tcgccttcgc cctgatagtt ttcgataacc
      781 agtttcaccg gatcgataac cgccattgcg cgcggggcgt tttcgttgag atcttcacgg
      841 atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt
      901 ttgcagaact cacgaataga agcagcagtg taaccacgac gacgcagacc ggaaatggtc
      961 ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag gttcagctta
     1021 cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcgggtga
     1081 acaggaatgg tgatgttgtc cagcacccag tcgtacaggc gacggttgtc ctggaactca
     1141 agcgtacaca gagagtgcgt aataccttcc agggcatcgc tgatgcagtg ggtgaagtcg
     1201 tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg
     1261 tacagcaccg gatcgcgcat cacgataaac ggtgaagcca tgtcgatttt cgcacgcagg
     1321 caggctttac cttcttcaaa accaccggca cgcatttttt cgaacagcgc caggttctct
     1381 tcaacgctgc ggtcgcggta cgggctgttt ttacccggtt gcgtcagggt gccgcggtat
     1441 tcgcggatct gttccggcgt cagttcatca acgtacgcca ggcctttatt gatcagttcg
     1501 atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag
     1561 tgaaaaccta accactctac gtcgtttttg atcgactcaa catactcgat atcttctttt
     1621 accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg
     1681 aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg
     1741 aaacgggtgt gtactgtggt gtgcttacca ctggccagat cttcatcgat gatctgacgg
     1801 ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa
     1861 cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt tagtgaaaat
     1921 cgaaaatctc actgagatta tggggatgat tgatgaaaaa aaaagcggca gagaagatct
     1981 ccaccgcttt tgccagtcct gcgtcattgt tggatgcgac gcttaagcgt cttatccaac
     2041 ctggactgag cttttgtagg cctgataaga cgcgtcaagc gtcgcatcag gcataaagca
     2101 gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc ctgagcttta
     2161 atgatcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat catcgagcgg
     2221 gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc gcttacctgc
     2281 gcaccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt gtcgataccc
     2341 atatggacga cgatctccgc gcctttttcg gtttccaggc agaacgcgtg gttggtgttg
     2401 aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt cggtttcacc
     2461 gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg atccagtgcc
     2521 acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt tggtacagcc
     2581 tgcggctttg ctacaggcgc ggcagttgcc ggagttgctt cagctgacgc agcggctacc
     2641 ggaccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt cgcgccaaca
     2701 atcacctgaa tagtctgttt gttcagtttc actacaccag aagcacccag acgtttacac
     2761 atcgtatcgt tgacgcgggc agagtcagcc acggtcaggc gcagacgggt gatacaggcg
     2821 tcaattgctt tcaggttgtc agtgccgcca accgcagcaa tatagttggt cgccagttga
     2881 ttgaggcctt cttcagtgtt gctgttggct tcttcagtaa cgatctcgtc ttctttatct
     2941 tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac cacgaagtag
     3001 atagcgaaga agacaacacc catcaccagc agcatccaga cgttctggct ggcggccggc
     3061 aggttataca tcaacgcgta gtcgatagcc cccgcagaga aagagaagcc agcatggata
     3121 cccagcagtg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag gaggtacagc
     3181 agcggtgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt caggaacgca
     3241 gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt cggtgctgcg
     3301 aagtacatcg ccagcgccgc acccggcaga ccgaacatca taatcgggaa gaagccggac
     3361 atgaacatcc ccgcggtgcc gtcaccggca tagaagcggt tgatatcacc gtggaaaacc
     3421 gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt cagcacctga
     3481 tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga acccagcgcg
     3541 cccgcagaaa cgatccactc gcctcctgca tggatagcgt gctgtaccgg cggccagacg
     3601 taaccaaaaa tggccgccag caccaggcag aagaagccgg tggcaatcgg cacaaagcgt
     3661 ttgccgccga agaaactcag gaagtccggc agtttaatat cagaccaacg gttataggct
     3721 gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat ttctgggttg
     3781 atggtcacca tcgctttggt taacacaaag taacctaccg cacccgccag cgccgccgca
     3841 cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa tccgaggtta
     3901 tcaaaaatcg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc tggctgaccg
     3961 aatcgcagca acagtgccgc caccggcagc accgcgatag ggagctgtaa cgccctaccg
     4021 agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc ctatttggct
     4081 cgtttaaagc cgtattttta ttttgctgca aattgtactg ccgatgttct gtaatcagat
     4141 tgttagatca tctgctacag agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgcgt
     4201 gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac tggctaaagc
     4261 agaaaactta ttttatcatt caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc
     4321 aggttacgct taaagatgcc taatccgcca acggcttaca ttttacttat tgaggtgaat
     4381 aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg ctcgccatat
     4441 cgtcaatcgt atcaatgcgt tcaaaccgac tgccgatcgt ccgtttgttc tgggcctgcc
     4501 gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata aagcaggcca
     4561 ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga
     4621 gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg ttgatattcc
     4681 agcagaaaac atcaaccttc tcaacggcaa cgccccggat atcgacgccg aatgccgcca
     4741 gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg gtgtaggtaa
     4801 cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa
     4861 aaccttgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca
     4921 ggtgccaaaa tatgccctga ctgtcggtgt tggtacattg ctggatgccg aagaagtgat
     4981 gattctggtg ctgggtagcc agaaagcact ggcactgcag gctgcagttg aaggttgcgt
     5041 gaaccacatg tggaccatca gctgtctgca actgcatcca aaagcgatca tggtgtgcga
     5101 tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg aattagaagc
     5161 agaaaatatc aaagatctgt aattgttatc cctgccctgg ctccttgctc agggcaatat
     5221 tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt taccggccac
     5281 gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag cgtctgtccg
     5341 gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat tctctccccc
     5401 ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga caccgctgaa
     5461 gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc aggctgtact
     5521 aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt
     5581 atgcgcgagt acctggcaag acatccgaat caggcgttag gtctgcatct ggaaggtccg
     5641 tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa gcctgatgcc
     5701 gcgctggtcg atttcctgtg taagaacgcc gacgtcatta ccaaagtgac cctggcaccg
     5761 gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc
     5821 ggtcactcca acgcgacgtt gaaagaagcg aaagccggtt tccgcgcggg gattaccttt
     5881 gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg cctggcgggc
     5941 gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct gcatgttgat
     6001 tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct ggttaccgac
     6061 gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa aacaatatac
     6121 taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc cttaaccatg
     6181 attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga agtgctgcgt
     6241 atggcgacgc tctatccggc gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc
     6301 gcaggtaaag tagccaacct gaccgcattc acacctgatt ttaaaatcac caagaccatc
     6361 gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc ggacaagctc
     6421 agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg
     6481 accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt gcccccgcca
     6541 gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa gttgatcagc
     6601 aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc aatttccacg
     6661 caatcggcgt acggcttggt cgtcacgacg ccaccatcac cctgtttgat ctcagcagca
     6721 aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcaaacg ctggaacatg
     6781 ccctgttgaa tgccattgct cagtttattg atagctacca gcgcaaactg cgcgagctga
     6841 tcgcgatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa attcattaca
     6901 tgccgcatat tcaggtagaa aactgggggc tggtagaagc tctggaagaa cgttttaaag
     6961 tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac tacttcggtg
     7021 caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc ggggccggga
     7081 ttatctctaa cgggcgtatt tttattggcc gcaacggtaa cgtcggtgaa attggccata
     7141 ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc ctggaaacta
     7201 tcgccgccaa cgctgccatt gaacaacggg tgttgaatct gttaaagcag ggctaccaga
     7261 gccgcgtgcc gctggacgac tgcaccatca aaactatctg caaagccgcg aacaaaggcg
     7321 atagtctggc gtcggaagta attgagtatg tcggtcgtca tctgggtaaa accatcgcca
     7381 ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg
     7441 ataaagtgct gctccctgct attgaaagct gcattaatac ccaggcgctg aaggcgtttc
     7501 gtactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc ggcgcttttg
     7561 cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg gaaaattaat
     7621 gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga ccattaaaaa
     7681 tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc
     7741 ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga ccaactatcc
     7801 ttcgcagacc gggcaagatc tggcgaaccg ctttgccact gccggtgtcg atgtgccgga
     7861 tagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa
     7921 gaaagcatat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag ccggtttcac
     7981 tattaccgat gtgaaccctg atttcgtgat tgttggcgaa acgcgttcct acaactggga
     8041 catgatgcat aaagcggcct atttcgtcgc taacggtgca cgctttatcg ccaccaaccc
     8101 ggacacccac gggcgcggtt tttatccggc ctgtggcgcg ttgtgtgcag ggattgagaa
     8161 aatctccggg cgcaaaccgt tctatgttgg taagcccagc ccgtggatca tccgcgcagc
     8221 attaaacaaa atgcaggcgc attcggaaga aacggtgatt gtcggcgata acttgcgcac
     8281 cgacattctg gcgggcttcc aggccggtct ggagacgatt ctggtgcttt ctggtgtttc
     8341 atcgctcgac gatatcgaca gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc
     8401 tgaaatcgac gttatctgaa taccacccac gtctgcggac gtggttggct ctgatgacaa
     8461 acgccaaact gcctgatgcg ctacgcttat caggcctacg cagctcctgc aatatattga
     8521 attttcatgc ttttgtaggc cggataaggc gttcacgccg catccggcat gaacaaatcg
     8581 cactttatca gcaatttggc ccccgtaaaa tataaccatt caataaaacc cgctaaaaat
     8641 tatcgattca tcaataaata catcctcaca ccagaccctt tttcaccaat cgacaatcac
     8701 cattacgttt tttatttttt caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat
     8761 ttttttaaac aagcaaacac aacaagcaac aaataccagg ttaacggaga aggttatgtg
     8821 ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta agaaagcact
     8881 cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt atgccagtga
     8941 taacgccatt ctcgcccacg aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc
     9001 tctctacaac caacaaaaaa cccacgtact ggcggtaaac ggtgaaatct acaaccacca
     9061 ggcattgcgc gctgaatatg gcgatcgtta tcagttccag acagggtctg actgtgaagt
     9121 gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc agggcatgtt
     9181 tgcctttgcc ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg accatctggg
     9241 gatcatccca ctgtatatgg gctatgacga acacggtcag ctgtatgtgg cctcagaaat
     9301 gaaatcgctg gtgccagttt gccgcacgat taaagagttc ccggcgggga gctatttgtg
     9361 gagccaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact ttgatgcagt
     9421 gaaagataac gtgaccgaca aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag
     9481 ccatctgatg tctgatgtgc cttacggtgt gctgctttct ggtggtctgg attcctcaat
     9541 tatttccgct atcaccaaga aatacgccgc ccgtcgcgtg gaagatcagg aacgctctga
     9601 agcctggtgg ccgcagttac actcctttgc tgtaggtctg ccgggttcac cggatctgaa
     9661 agcagcccag gaagtggcaa accatctggg cactgtgcat catgaaattc acttcactgt
     9721 acaggaaggt ctggacgcca tccgcgacgt gatttaccac atcgaaacct atgatgtgac
     9781 cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaaag cgatgggcat
     9841 taaaatggtg ctgtccggtg aaggttctga tgaagtgttc ggcggttatc tttacttcca
     9901 caaagcaccg aatgccaaag aactgcatga agagacggtg cgtaaactgc tggccctgca
     9961 tatgtatgac tgcgcgcgtg ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt
    10021 tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgt attaacccac aggataaaat
    10081 gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgaag cgtatctgcc
    10141 tgcaagcgtg gcctggcggc agaaagagca gttctccgat ggcgtcggtt acagttggat
    10201 cgacaccctg aaagaagtgg ctgcgcagca ggtttctgat cagcaactgg aaactgcccg
    10261 cttccgcttc ccgtacaaca cgccaacctc taaagaagcg tacttgtatc gggagatctt
    10321 tgaagaacta ttcccgcttc cgagcgccgc tgagtgcgtg ccgggcggtc cttccgtcgc
    10381 ttgttcttcc gctaaagcga tcgaatggga tgaagcgttc aagaaaatgg acgatccgtc
    10441 tggtcgcgcg gttggtgttc accagtcggc gtataagtaa gaaatgcgaa acagccccgg
    10501 tgaaatccgg ggctgtttca gttattgagg tgcttgttga ttctgatatg cagtaaggaa
    10561 atgttccggg tttgccacat cactgtttga caactcatca ctgtaggcca gtatttcata
    10621 gattaatttc ccaccactat tgttaaattc cgaatttcat cctgtatgac aaaagaatgg
    10681 ctgtagggtg ggaaaaaaca caaagtaaac aataattgac gaatatagcg ccacgctgtt
    10741 cgcaacctaa ccaaacagtc actttcgagc aattttcctt gaaaaagagg ttgacgctgc
    10801 aaggctctat acgcataatg cgccccgcaa cgccgataag gtatcgcgaa aaaaaagatg
    10861 gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat
    10921 cccgtcgtag ccaccatctt ttttgcggga gtggcgaaat tggtagacgc accagattta
    10981 ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct cccgcaccat tcaccagaaa
    11041 gcgttgtacg gatggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    11101 ctggttcgaa tccaggtacc ccagccatct tcttcgagta agcggttcac cgcccggtta
    11161 ttggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat
    11221 ccaggtaccc cagccatcga agaaacaatc tggctacgta gctcagttgg ttagagcaca
    11281 tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccaaa ttctgaatgt
    11341 gtcgaatatg ttcggcaaat tcaaaaccaa tttgttgggg tatcgccaag cggtaaggca
    11401 ccggattctg attccggcat tccgaggttc gaatcctcgt accccagcca atttattcaa
    11461 gacgcttacc ttgtaagtgc acccagttgg ggtatcgcca agcggtaagg caccggattc
    11521 tgattccggc attccgaggt tcgaatcctc gtaccccagc cacattaaaa aagctcgctt
    11581 cggcgagctt tttgcttttc tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta
    11641 tccttcaatg tcggatgcga cgctgccgcg tcttatccga cctacggttg gcacgcatcc
    11701 ggcaatgttg taaggctaca accctaacgc atatttcagc gcctgacgtt tcaacacgcc
    11761 agcacgctcc gccgccatta acccgagatt acgcataaaa cgcagtggtg gcagattatt
    11821 gctgaatccg gcataaaaca gatccatacc gctttgcata atgaagttat ccgccatgcg
    11881 ccgcatctgg tagcgcttga ggacaggata actggcccac gcttcgccgt agctgcgggc
    11941 attcaccaga acatcaatca gggcatcgac atcacgataa ccaagattca ccccctgccc
    12001 cgccagcgga tggatggtat gcgcggcatc gcccaccagc gcaagccccg gctgcacata
    12061 ctgcaccgca tgtcggcgcg tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc
    12121 cagacgcgac gggaaatgct tcgcgatttc cgcctggagt tgcgccatat tcatattctg
    12181 caactggcga atacgcaccg gagagtcata ccacaccagc gatgcccagt tatcaaacaa
    12241 cggcagaaac gcacgcggtc cgtccggggt aaattgctgc caggtgctgt cgccgggatc
    12301 gttctcacac tgtacgctaa tcaacataca cgactgcgca tactgccagg catgaacgcc
    12361 aattcccgcc atctgccgca cctgcgaatt tgcgccgtca gcaccaatca ccagcttcgc
    12421 gcgaatcact tcaccgccct tcagttccag ctcctgaaga tcattatctc gatgtaatgc
    12481 aatcagcgag cctggcacac gtaacgttac tttcggatga gcttccagcg cctgccacaa
    12541 tgcctgttgc aggacggtgt tttccaccat atagccaagc aacggtagct taagttcagc
    12601 ggcgtcaaac atcacatgcg ccgtttccca ctcccacgtt tccagtctgc ggtaaagatg
    12661 gcaacgcata gcctgtactg catcccagac ccctaaccct ttaagcaatg ataccgatgc
    12721 ggcgctgatc gccgagatcc gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc
    12781 tgcgtgctcg atcaccgtta ccgaaaatcc gtgctgtgcc agccccagcg ccagtgcgcc
    12841 gccgaccatt cctccgccga caatggcaat ttccgttggt tgatttgtca tggtcaatca
    12901 tcctgttagc aatatgcttt aagtttacag gatttttacc ctcaagggct gataacgctc
    12961 atactggtca caacggcagc aaagcattac actatgcgcc ctgcattcct ggctactatt
    13021 tcgcaagagc aagtcgatga ccaaaaaact ccatattaaa acctggggct gtcagatgaa
    13081 cgagtacgat tcatcgaaga tggccgatct gctggatgcc acccacggct atcaactgac
    13141 cgacgtggcg gaagaagcgg atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc
    13201 tcaggaaaaa gtcttccatc agttgggtcg ctggaaactg ttaaaagaga agaatccaga
    13261 cctgattatc ggcgtcggtg gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg
    13321 cgcccactat gtcgatatta tttttgggcc gcaaacgctg caccgtctgc cggagatgat
    13381 caactccgtg cgcggcgacc gcagcccggt tgtagatatc agcttcccgg aaatcgagaa
    13441 gtttgaccgt ctgccggaac cgcgcgccga agggccaacc gcgtttgtct ccatcatgga
    13501 aggctgcaat aaatattgca cctactgcgt ggtgccttac acccgtggtg aagaggtaag
    13561 ccgtccgtcc gacgatattc tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga
    13621 agtcaacctg ctcggtcaga acgtgaatgc ctggcgtggt gaaaactacg acggcaccac
    13681 cggatcgttt gccgatctgc tgcgtctggt tgcagcgatc gacgggatcg atcgtattcg
    13741 ctttaccacc agtcatccga tcgaattcac cgacgatatc atcgaagtgt accgcgacac
    13801 gccggagctg gtgagcttcc tgcatctgcc ggtacagagc ggctccgatc gcattctgaa
    13861 cctgatggga cgtacccata cggcgctgga gtacaaagca atcatccgta cactgcgtgc
    13921 agcgcgcccg gatattcaga tcagttctga cttcatcgtc ggcttccctg gcgaaaccac
    13981 cgaagacttc gagaaaacga tgaagctgat tgccgacgtc aatttcgaca tgagctacag
    14041 ctttatcttc tctgcacgtc cgggtacacc agccgccgat atggttgatg atgttccgga
    14101 agaagagaag aagcaacgtc tgtatattct gcaagaacgt atcaatcagc aagcgatggc
    14161 atggagccgc cggatgctcg gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa
    14221 gagcatcatg gagctttccg ggcgtaccga aaataaccgc gtggtcaact tcgaaggcac
    14281 gccggatatg atcggtaaat tcgtcgatgt agaaattacc gacgtctacc cgaactctct
    14341 gcgcggtaaa gtggtgcgaa ctgaagatga aatgggtctg cgcatggcag aaacaccgga
    14401 gtcggtgatt gcccgcaccc gcaaagaaaa cgaccttggc gtgggttatt atcagccgta
    14461 attctcaggc ctgccgttcc ggtgggcctt gttttttctc tctttatccc catctttatc
    14521 gcaatgcttg cgcctttttc ctgtggcgtg aataatttcc ttataggccg gtgaatgttc
    14581 agcttccggc ggcatacaga taagaggaac ggtttgaaca tagacactcg cgaaatcacc
    14641 ctggagccaa cagacaatgc gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc
    14701 aagcagctcg aacgccgtct cggcatcgag atcaatcgcc gcgataacca ctttaaactg
    14761 accggccgtc cgatttgcgt caccgctgcg gcagacattc tgcgtagcct gtatgtcgat
    14821 acagccccga tgcgcggtca gattcaggat atcgaaccgg aacagatcca ccttgcgatt
    14881 aaagaagcgc gggtacttga gcaaagcgcg gagagcgtgc cggagtacgg caaagcggtc
    14941 aatataaaaa ccaaacgcgg cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc
    15001 gccaatattc tcgaccatga cattaccttc ggcgttggcc cggcgggtac gggtaaaacc
    15061 tacctggcag tggctgcggc agttgatgcc ctggagcgtc aggaaattcg ccgtattctg
    15121 ctgactcgtc cggcggtcga agccggtgag aaactgggct tcctgcctgg cgatttaagc
    15181 cagaaagtag acccgtatct gcgcccactg tacgacgcac tgtttgaaat gctgggcttt
    15241 gagaaagtcg agaaactgat tgagcgcaac gttattgaag tcgcgccgct ggcctatatg
    15301 cgtggtcgta cgctgaatga cgcctttatc attctcgatg agagccagaa caccaccatc
    15361 gaacagatga agatgttcct gacccgtatc ggttttaact caaaagcggt tatcaccggc
    15421 gacgtcacac agatcgacct gccgcgtaat actaaatcag gcttacgtca cgccatcgaa
    15481 gtgctggccg atgtcgaaga gatcagcttt aacttcttcc acagcgaaga cgtggttcgt
    15541 cacccggtgg tggcgcgtat cgttaacgct tatgaagcct gggaagaagc cgaacaaaaa
    15601 cgaaaagcgg cgctggcggc agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg
    15661 tgatcctcga tttacaactg gcatgtgaag ataattccgg gttaccggaa gagagccagt
    15721 ttcagacatg gctgaatgcg gtgatcccgc agtttcagga agagtcggaa gtgacgattc
    15781 gcgtggtcga taccgccgaa agccacagtc tgaatctgac ctatcgcggt aaggataagc
    15841 cgaccaacgt gctctccttc ccgtttgaag tgccgcctgg catggagatg tcgctactgg
    15901 gcgatctggt tatctgccgt caggtggttg agaaggaagc tcaggagcaa ggcaaaccac
    15961 tggaggcgca ctgggcgcat atggtggtgc acggcagtct gcatttgtta ggttacgatc
    16021 acattgaaga tgacgaagca gaagaaatgg aagccctcga aacagagatt atgcttgctc
    16081 tgggctatga ggatccgtac attgccgaga aagaataatt tgccaggcat ttgactggca
    16141 gacgtccccg ccgttggtga ttaacgacgg cgttttgatt aactaacatg agaacccata
    16201 cgacgccatg agcgacgaca attcacacag tagtgacacg ataagcaaca agaagggatt
    16261 tttctccctg ttactcagcc aacttttcca cggtgaaccg aaaaaccgtg acgaactgct
    16321 ggcgctgatc cgtgattccg ggcagaacga ccttatcgac gaagatacgc gcgatatgct
    16381 cgaaggggtg atggacatcg cagaccaacg cgttcgcgac atcatgatcc cccgctccca
    16441 gatgattacc ctgaaacgca accagacgct ggacgaatgc cttgatgtca tcatcgagtc
    16501 cgcccactca cgtttcccgg tgattagcga agacaaagat cacattgaag ggattctgat
    16561 ggcgaaagac ttgctgccgt ttatgcgcag cgatgctgaa gccttcagca tggacaaagt
    16621 gttacgtcag gcggttgtcg ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt
    16681 tcgctctcag cgttaccaca tggcgatcgt tattgacgaa ttcggtgggg tttccggtct
    16741 ggtgaccatt gaagacatcc tggaactgat tgttggtgag attgaagacg agtatgacga
    16801 agaagatgat atcgacttcc gtcagctgag tcgtcatacc tggaccgtgc gcgcactggc
    16861 ttccattgaa gacttcaacg aagcgttcgg cacccacttt agcgatgaag aggttgacac
    16921 tatcggtggt ctggtgatgc aggcatttgg gcatcttccg gcgcgtggcg aaaccatcga
    16981 catcgacggt taccagttca aagtggcgat ggccgacagt cggcgtatta ttcaggttca
    17041 tgtcaaaatc ccggatgact caccccagcc aaagctggat gaataaaacc gaaactggat
    17101 agataactac atggcttttg cctcattaat tgaacgccag cgcattcgcc tgctgctggc
    17161 gttattattc ggtgcctgcg gaacgctggc cttctctcct tacgacgtct ggcctgcggc
    17221 gattatttcg ctgatggggc ttcaggcgtt gacctttaac cgtcgtccac tccagtctgc
    17281 cgctattggc tttggctggg gatttggcct ctttggcagc ggtattaact gggtctatgt
    17341 cagcatcgcg acctttggcg gaatgcctgg cccggttaac atcttcctgg tggtactgct
    17401 ggcggcgtat ttgtcgctgt ataccggact gtttgctggc gtgctgtcgc gtctgtggcc
    17461 aaaaaccacc tggctgcgcg tggcgattgc cgcccctgct ctctggcaag tgaccgagtt
    17521 tctgcgcggt tggatactga caggcttccc gtggttacag ttcggctata gccagattga
    17581 tggcccgtta aaagggctgg caccgataat gggcgtggaa gccattaact tcctgctgat
    17641 gatggttagt ggcctgctgg cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt
    17701 ggccgtcgtg ctgtttgccc tacccttccc gctgcgttac atccaatggt ttaccccgca
    17761 accggagaaa accattcagg tttcgatggt tcagggcgat attccgcaat cactgaaatg
    17821 ggatggagat cagcttctta atacgctgaa gatttactac aacgcaacgg ctccgctgat
    17881 gggcaaatca tcgttgatta tctggccgga gtcggcgata accgatctgg aaattaatca
    17941 gcaaccgttc ctcaaagcac tggacggtga gttgcgcgat aaaggtagct cgctggtaac
    18001 cgggattgtc gacgcgcgtc tcaataagca gaaccgctac gatacctaca acaccatcat
    18061 cacgctgggt aaaggtgcac cgtacagcta cgaatcagcc gatcgctata acaaaaacca
    18121 tctggtgccg tttggcgagt ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt
    18181 ctttgatctg ccgatgtcgt cgttcagccg tgggccatat atccagccgc cgctgtcggt
    18241 aaatggtatt gagcttactg cggctatttg ctacgagatc attctcggcg agcaagtgcg
    18301 cgataacttc cgcccggata ccgactacct gctgactatc tccaacgatg catggtttgg
    18361 taagtctatt ggtccatggc agcacttcca gatggcgcga atgcgtgcgc tggagctggc
    18421 gcgcccattg ctgcgtagca ccaacaacgg cattacggcg gtgattggcc cgcagggtga
    18481 gattcaggcg atgatcccgc agttcacccg cgaggtgttg accactaacg tgacgccgac
    18541 gaccggactc acaccgtacg cacgaaccgg caactggccg ctgtgggtgc tgacggcgct
    18601 gtttggtttt gccgctgtgc tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg
    18661 cgctacgctt atcaggccta cgcgattttt gcaatatgtt gaatttacgt gattttgtag
    18721 gataaagcgt actttctcaa cacgctgtgg ggcatgatta tcatgcccct ttttcttacg
    18781 gttcagggat acaagccaca ttgccatatt ggggatgacg aataactgcc tgcggatcaa
    18841 gcgagagcgt cctgtccatt tggattaacc cgttcttcgc cataacaaat atctttctac
    18901 catccgccga aaaacgaagg tgattaacgt agctggcagg ataaacgcca acaatctgcc
    18961 cgacattaat attgagcagg atgacataac cttgcaagcc atatgacacc aacgctaaat
    19021 actgaccgtc cgggctaata ccctcaacgg aataaacgct cctgctacac cattgcgtca
    19081 taatgctggc ttgctgatcc ccaactctta tacgtgaaat cccctggctg tcggcaaaaa
    19141 ttacatcccc gttttcaggt cgaaaacgca tcgccgatat cgttccacgc gcatcacctg
    19201 cgggaaagtc ttctttctga tgcgtgatgg tattgatgcg tacaatgtca cctctgacca
    19261 taccgggtat caacaatgtt ttttcatccg gggaaagtgc cataacccag gcaccaggtt
    19321 taccgctcgt ccccgtcccg tcgtatttct ccagcgggat atccgccaca tgctgataag
    19381 tcaccagatc aatcgctgac acactattac tggtgcgatt catcacccag gcttttttgc
    19441 catcacgcga gaggataagg ctgtcaggat gtgcaccggt ggtaatcgac tgaataatct
    19501 cccccgtcgc cacgttaatg accgataacg cattcgcttc aaatgaagtc gcataaagca
    19561 acttttcatc cgggcttaac gccagcgatc tgacgccctg atctttacag ccaccacaat
    19621 gtttgactga cttttctttc gctatgggcg ctgtaaaggt atgcaggatt ttcttttgtc
    19681 cgaggtcgaa cacaacaatg ctgtttgtgt tttcaatagc ggcatataac gtattaccat
    19741 cccgactgat cgccatatca tggatatcgg caatctcagc gagcaaatca ccactggcct
    19801 gttgacgact taccggagcg ggtaatgttt ctttgagggc gttgcgttcc tccgctaact
    19861 gttgcttttt ttgtcgtcgc tcttgttcaa tctcctgcgg ataaaacgct tcacgcaggc
    19921 tttgcctgac ctgtaaaact gattcatcaa tctgctgctg cgcaagccag atagccccgg
    19981 taagaagaag actgcccgca acaataatcc cgccttccgt aacaagccgg ttttgcccag
    20041 ataaacgcgc actcagccaa cccaacgcgc cacccgcaat caggccgcca atgtgacaag
    20101 cattatctat tcctgtttgc cgggcaccat acaacagtgt cagcgccacc atacctaaca
    20161 gagaaaatac tcgccgctga tttttatggt gcgtgcccgt acctgaagca agctgtgttg
    20221 ctatcgcagc gccagctatt cccataattg ctccggatgc cccaacgctg atgactatct
    20281 gctcactatc gcgtaacgca taacaggcgc tgatgagccc accaaagacg ccggagaata
    20341 accagataat caggagtcgc catttcccca gtatccgttc cgccactgcc cccaccacca
    20401 ggaatgcaaa catattcatc agcagatgag aaacactgct gtgcagaaag acactgctca
    20461 ataaacgcca gggttcaccc gacaacgtga gagaagcaac gttagcgccg agatggataa
    20521 gtgcggattg atcgacttct tcataactca tccattgtgc gaggtaacaa aatatcccaa
    20581 cacagagcgc aaaaaggata agggttattg cgggtaattg aacatttaac ggcttaaccg
    20641 aagatgcaga cataagatga ttcttccttg aaaagacagg taaaaagcaa ttttcccgtg
    20701 tgcagttatc atcccggcaa acgtcccata ccgaaaagtt aaccctgtcc gaaatatgac
    20761 tttttcagcc tgaaattaca atcgtaatta attcacttct aagtattttt tggcatgtaa
    20821 gttgctttat ttactgtgta agcaatgaaa tcaaccgcga cgcaaccggg ttgcaccacc
    20881 acaaatcagc ggtagcacca aaaaggtgcg gtttaagaat tttgcttcgg aatggtgcgg
    20941 cgcatctcgc aaaaacaaac aataaaacag caatatcttt acatttagcc aaactaaatg
    21001 ttacctaaca ggcacaacac tgcacaataa agttgcagac gataacaaca caaacactca
    21061 caacgggtat ccatgcgttc ttaacgcaga agataaagga gttggatatg caattacgta
    21121 aacctgccac agcaatcctc gccctggcgc tttccgcagg actggcacag gcagatgacg
    21181 ccgccccggt agcgggcagt acgctggaca aaatcgccaa aaacggcgtg attgtcgtcg
    21241 gtcaccgtga atcttcagta cctttctctt attacgacaa tcagcaaaaa gtggtgggtt
    21301 actcgcagga ttactccaac gccattgttg aagcagtgaa aaagaaactc aacaaaccgg
    21361 acttgcaggt aaaactgatt ccgattacct cacaaaaccg tattccactg ctgcaaaacg
    21421 gcactttcga ttttgaatgt ggttctacca ccaacaacgt cgaacgccaa aaacaggcgg
    21481 ctttctctga tactattttc gtggtcggta cgcgcctgct gaccaaaaag ggcggcgata
    21541 tcaaagattt tgccgacctg aaaggcaaag ccgtagtcgt cacttccggc actacttctg
    21601 aagttttgct caacaaactg aatgaagagc aaaaaatgaa tatgcgcatc attagcgcca
    21661 aagatcacgg tgactctttc cgtaccctgg aaagcggtcg tgccgttgcc tttatgatgg
    21721 atgacgctct gctggccggt gaacgtgcga aagcgaagaa accagacaac tgggatatcg
    21781 tcggcaagcc gcagtctcag gaagcctacg gttgcatgtt gcgtaaagat gatccgcagt
    21841 tcaaaaagct gatggatgac actatcgctc aggtgcagac ttccggtgaa gcggaaaaat
    21901 ggtttgataa atggttcaaa aatccaattc cgccgaaaaa cctgaacatg aatttcgaac
    21961 tgtcagacga aatgaaagca ctgttcaaag aaccgaatga caaagcactg aactaattac
    22021 aagaaccagg ggcggaaatt ctagccctct cgattgttac gtagcacgga cagactatac
    22081 gcctgatggt cgttccccat cgggcctgaa aaccgcaata cgctgggtaa caatcttcga
    22141 gggtagcagt taacgctgct accctctttt ttctggagta gatttatgtc tatagactgg
    22201 aactggggta tttttttaca acaagccccg ttcggcaaca ccacctatct tggttggatc
    22261 tggagtggtt ttcaggtgac gatcgctttg tcgatctgcg cctggattat cgctttcctc
    22321 gtcggttcat ttttcggcat tttacgtacc gttccaaacc gttttctctc cggtctgggt
    22381 acgttgtatg tcgaactgtt ccgcaacgtg ccgttgattg tgcaattctt tacctggtat
    22441 ctggtgatcc cggagctgct gccggagaaa atcggcatgt ggtttaaggc cgagctggat
    22501 cccaatattc agtttttcct ttcatccatg ctctgcctgg ggctgtttac cgccgcccgt
    22561 gtttgcgaac aggtacgcgc cgctattcag tcgctgccgc gtgggcagaa gaatgccgcg
    22621 ctggcgatgg gtctgacgct accgcaggct taccgctacg tgctgttgcc taacgcgtat
    22681 cgcgttatcg tcccgccaat gacttccgag atgatgaacc tggtgaaaaa ctccgccatc
    22741 gcctcgacta tcggtctggt ggatatggca gcgcaggcgg gtaaattgct cgactactcg
    22801 gcccacgcct gggagtcgtt taccgccatt acgcttgcgt atgtgttgat taacgctttt
    22861 atcatgctgg tgatgacgct ggttgaacgt aaagttcgcc tgcccggcaa catggggggc
    22921 aaataatgta cgagtttgac tggagttcca ttgttccttc cctgccatat ctgctcgacg
    22981 ggctggtgat caccctgaaa atcaccgtca cggcagtcgt gattggtatt ttgtggggta
    23041 ccatgctggc ggtgatgcgc ttgtccagct tcgcgcctgt ttcgtggttt gccaaagcct
    23101 acgttaacgt attccgctcg attcctttag tcatggtttt gctgtggttt tacttgattg
    23161 tgccgggatt tctgcaaaat gtgctgggat tatcgccaaa aaatgatatt cgtttaattt
    23221 cggcgatggt ggcgttttcg atgtttgaag cggcctacta ttcagagatt atccgtgccg
    23281 gtattcaaag tatttctcgt ggtcagtcga gcgccgcgct ggcgttggga atgactcact
    23341 ggcagtcgat gaaactgatt attctgccgc aggcgttccg cgcgatggtg ccgctgctgc
    23401 tcactcaggg catcgtactg ttccaggata cctcgctggt gtatgtgtta tctctggccg
    23461 atttcttccg taccgcctca accattggtg agcgtgatgg tacgcaggtt gagatgatcc
    23521 tgtttgccgg atttgtttat ttcgttatta gtcttagcgc gtcgttgttg gtcagctact
    23581 tgaaaagaag gacagcataa tgattaccct gaaaaatgtt tcaaaatggt atggtcactt
    23641 tcaggtgctg accgactgct caaccgaagt gaaaaaaggc gaagtggtgg tggtttgcgg
    23701 cccgtctggt tccggcaaat caacgctgat taaaaccgtc aacggcctcg aaccagtgca
    23761 gcaaggtgaa atcaccgtcg atggtatcgt ggttaatgac aagaaaaccg atctggcaaa
    23821 gctgcgttcc cgcgtcggga tggtgttcca gcattttgag ttgttccccc atttgtcgat
    23881 tattgaaaac ctgactctgg cgcaggtgaa agtgcttaaa cgcgataaag cgcccgctcg
    23941 tgaaaaagca ttgaaattgc tggagcgcgt gggactttcc gcacatgcca ataaatttcc
    24001 ggcgcagctt tccggcggtc agcagcagcg tgtggcgatc gctcgcgcat tgtgtatgga
    24061 tcctattgcc atgctgttcg acgaaccgac atcggcgctg gatccggaga tgatcaacga
    24121 agtgctggac gtgatggtgg aactggcaaa cgaaggaatg accatgatgg tggtgaccca
    24181 cgaaatgggt tttgcccgta aagtggcgaa tcgggtgatc tttatggatg agggtaaaat
    24241 tgtcgaagac tcgccgaaag atgcgttctt cgatgatccg aaatcggacc gcgcaaaaga
    24301 cttcctcgcg aaaatcctgc attaatcact ctggcgcgca tacccaattg cgcgccatct
    24361 cacgctttat cgtttcgccc ctcgtatctg ttacatcctg gcgttaccct tgtcgcaaag
    24421 aagcacacaa caaggagcaa caatggcact gccaattctg ttagattgcg acccaggtca
    24481 tgacgacgct atcgcaatag ttctcgccct cgcctcacca gagcttgatg tcaaagcaat
    24541 tacgtcttcc gccggaaacc agacaccaga aaaaacctta cgcaatgttc ttcgtatgct
    24601 gaccttgctc aatcgcaccg atattccggt agctggcggc gcggtaaaac cgttaatgcg
    24661 tgagttgatt atcgcggaca atgtgcacgg cgaaagcggt ctcgacggcc cggcattacc
    24721 ggaaccggcg ttcgcaccgc aaaactgtac ggcggtagag ctaatggcga aaacgctgcg
    24781 tgaaagtgcg gaacctgtca ccattgtgtc taccggaccg caaactaacg ttgccttgct
    24841 gctcaatagc catccggaac tgcatagcaa aattgcccgt atcgtgatta tgggcggcgc
    24901 aatggggctt ggtaactgga cgccagcggc tgaatttaat atttacgttg acccggaagc
    24961 ggcagaaatt gtcttccagt cagggatccc ggtggtgatg gccggtctgg atgttactca
    25021 taaagcacaa atccacgttg aagacaccga gcgtttccgc gcgattggta accctgtttc
    25081 aaccattgtt gccgaactgc tggatttctt cctcgaatat cataaagacg aaaaatgggg
    25141 ctttgtcggc gcaccactgc atgacccatg caccatcgcc tggctgttga aaccggagtt
    25201 atttacctct gttgagcgct gggttggcgt ggaaacacag gggaaatata cccagggtat
    25261 gacggttgtt gattattatt atctgacagg caataaaccg aatgccaccg taatggtcga
    25321 tgttgatcgt cagggctttg ttgatttact ggctgatcgt ctgaaatttt acgcttaaca
    25381 cacgtcattg attaagatca ccacgcgtta cctgggtggt cttaatctgt ttatctcttt
    25441 ttctttcaag atataaatgc aatggataat gcagaactcg ccattggtat cgatctcggt
    25501 actaccaata gtttaattgc cgtctggaaa gacggtgccg cgcaattaat tccaaataag
    25561 ttcggtgaat atttaacacc atccataatt agcatggatg aaaataatca tattttagtc
    25621 ggaaaaccgg ctgtatcacg gcgtacttcg catccggata aaacggcagc gttatttaaa
    25681 cgtgcaatgg gcagtaatac caactggcgg ttaggcagcg acacatttaa cgcgccagaa
    25741 ctgtcctctt tggtattacg ctcattaaaa gaagatgccg aagaatttct gcaacgtccg
    25801 attaaagatg tggtgatctc cgttccggct tatttcagcg atgaacaacg caagcatacc
    25861 cgtttagcag cggagttagc cgggttaaat gcggtacgct taattaatga acccacagca
    25921 gctgcgatgg cgtatggcct gcatacccaa caaaataccc gctcgctggt ttttgatctc
    25981 ggtggcggca cgtttgacgt cacggtgctt gagtacgcca cgccggtgat tgaagttcac
    26041 gcctccgctg gcgataactt cctcggtggc gaagatttta cccatatgtt ggtcgatgag
    26101 gttttaaagc gcgcggatgt caccaggacc acgcttaacg agagtgaact ggcagccttg
    26161 tacgcctgtg tggaagcggc aaaatgtagc aatcaatcgc caatgcacat tcgctggcag
    26221 taccaggatg aaacgcggga atgcgaattt tacgagaacg aactggaaga tttgtggttg
    26281 ccgctgctca atcgcttgcg agtgccgatt gaacaggcgt tgcgcgatgc gcgtctgaag
    26341 ccgagtcaaa tcgacagtct ggtgctggtt ggcggcgcgt cacaaatgcc gctggtgcag
    26401 cgaatcgccg tgcgtctgtt tggcaaatta ccgtatcaaa gttacgatcc gagcaccatt
    26461 gtcgcgctgg gcgcagcaat ccaggccgcc tgccgcttac gcagtgaaga tattgaagaa
    26521 gtgatcctca ctgatatttg cccttactcg ttgggcgttg aagttaaccg ccagggcgtt
    26581 tccggcattt tctcgccgat tattgaacga aacaccacag taccggtgtc gcgtgtagaa
    26641 acttactcga ccatgcaccc ggaacaggat tctattacgg ttaacgtcta tcagggagaa
    26701 aaccacaaag ttaaaaataa cattctggtg gaatccttcg atgtgccgtt gaagaaaacc
    26761 ggggcttatc agtcgattga tattcgcttt agttatgata tcaacggctt gcttgaagtt
    26821 gacgtgcttc tggaagatgg cagcgttaag tccagagtca ttaatcatag cccggtaaca
    26881 ttgagcgcgc agcagattca agagagtcgg acgcggttat ccgcattgaa aatttatccg
    26941 cgcgatatgc tcatcaatcg cacctttaaa gccaaactgg aagagttgtg ggcgcgggcg
    27001 ctgggtgacg agcgagaaga gatcggccgg gtgatcaccg attttgatgc ggcgttgcag
    27061 tcaaacgata tggcccgcgt cgatgaggtc aggcggcggg cgagcgttta tttagccatt
    27121 gagaccccat aatcacggct gccggatgcg gcgtaaatgc tgcatccggc atcggcgcaa
    27181 aaaacacatc gtcaactaaa caacatcgcg attttgccga gagcataacc tataaccagc
    27241 accgttaaac tgattttgat aatgctggta atgctctgtg gtttttcacc ctcatcattt
    27301 ttttcttcat ggttttcagg tgttggctca tcgacatctt catcctccgg ctcgagtttt
    27361 actttcgtca acagcatccg ggtgataatg gcctctaatg gatgagcatc tccgccttcc
    27421 agccttgtat taagacgggc cagcatctct tgtttcggaa acgcttcaag tactacctgt
    27481 tccgctaccc gatacaaatc cagtaacggt gaatcttctt ctgtctcgct accgactctc
    27541 gatttccacc acgcccatgc tttgcgtttg aagaatagcg actcaataaa cgattgacct
    27601 aacggcgata attttccgct atgtaaaatt tccgcccata acagccgcgt ccgggcgctc
    27661 tcaccaggaa catatttcag cggttgacct tgcgcatcaa ccatgctctg cgcttcgata
    27721 accatcacta ataacgcgaa ataatccggg caatgacgct tgctccactg caacaacaaa
    27781 ttatcagctt gtgtagatgg gtaactctcc cagtatgcgc agagctcagc aagcaggctt
    27841 tccccctcgc cgcagtacaa acgttgtgca cactggtaat accgggcatc ttcgctttct
    27901 ggttcttccg cttgccactg tttggctaca ggaatgagtt ccgccatacc tgtttgcacc
    27961 gagctgtacc agcgcaacag tttgcgatgt aactttgcat catcaggaat gaaccacgga
    28021 ccttccatcg ccaaaaacgc agtgacatag tcaggatggt aacggcagca acgttccagc
    28081 gcgaagtaat actcaacagt ctggttctgc aacgcaatgg gatattccag aaggctaagg
    28141 aaatcgaaac aatcacca
//
LOCUS       DAOFQD010000059        28028 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.059, whole genome shotgun sequence.
ACCESSION   DAOFQD010000059 DAOFQD010000000
VERSION     DAOFQD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.059"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(103..693)
                     /gene="paaY"
                     /locus_tag="QBF46_002567"
     CDS             complement(103..693)
                     /gene="paaY"
                     /locus_tag="QBF46_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDQ6674733.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHSCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVESGRKRLVFDENLRPKQ"
     gene            complement(675..1625)
                     /gene="paaX"
                     /locus_tag="QBF46_002568"
     CDS             complement(675..1625)
                     /gene="paaX"
                     /locus_tag="QBF46_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDQ6674734.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            complement(1726..3039)
                     /gene="paaF"
                     /locus_tag="QBF46_002569"
     CDS             complement(1726..3039)
                     /gene="paaF"
                     /locus_tag="QBF46_002569"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDQ6674735.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     NAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(3066..4271)
                     /gene="paaJ"
                     /locus_tag="QBF46_002570"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(3066..4271)
                     /gene="paaJ"
                     /locus_tag="QBF46_002570"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDQ6674736.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRSAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(4271..4693)
                     /gene="paaI"
                     /locus_tag="QBF46_002571"
     CDS             complement(4271..4693)
                     /gene="paaI"
                     /locus_tag="QBF46_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDQ6674737.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(4683..6110)
                     /gene="paaC"
                     /locus_tag="QBF46_002572"
     CDS             complement(4683..6110)
                     /gene="paaC"
                     /locus_tag="QBF46_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDQ6674738.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FEQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(6112..6900)
                     /gene="paaB"
                     /locus_tag="QBF46_002573"
     CDS             complement(6112..6900)
                     /gene="paaB"
                     /locus_tag="QBF46_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDQ6674739.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(6900..7667)
                     /locus_tag="QBF46_002574"
     CDS             complement(6900..7667)
                     /locus_tag="QBF46_002574"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDQ6674740.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(7664..8734)
                     /gene="paaK"
                     /locus_tag="QBF46_002575"
     CDS             complement(7664..8734)
                     /gene="paaK"
                     /locus_tag="QBF46_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDQ6674741.1"
                     /translation="MTTFHSLTVAKVESETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAETALKALGMPEKAIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(8742..9239)
                     /gene="paaD"
                     /locus_tag="QBF46_002576"
     CDS             complement(8742..9239)
                     /gene="paaD"
                     /locus_tag="QBF46_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDQ6674742.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTNGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLREYGISPPAGHCCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(9254..10000)
                     /gene="paaC"
                     /locus_tag="QBF46_002577"
     CDS             complement(9254..10000)
                     /gene="paaC"
                     /locus_tag="QBF46_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDQ6674743.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(10009..10296)
                     /gene="paaB"
                     /locus_tag="QBF46_002578"
     CDS             complement(10009..10296)
                     /gene="paaB"
                     /locus_tag="QBF46_002578"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDQ6674744.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(10308..11237)
                     /gene="paaA"
                     /locus_tag="QBF46_002579"
     CDS             complement(10308..11237)
                     /gene="paaA"
                     /locus_tag="QBF46_002579"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HDQ6674745.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLTWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQHARKVA"
     gene            11522..13567
                     /gene="paaZ"
                     /locus_tag="QBF46_002580"
     CDS             11522..13567
                     /gene="paaZ"
                     /locus_tag="QBF46_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDQ6674746.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHLLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            13815..16088
                     /gene="tynA"
                     /locus_tag="QBF46_002581"
     CDS             13815..16088
                     /gene="tynA"
                     /locus_tag="QBF46_002581"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDQ6674747.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTAVEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(16146..17645)
                     /gene="feaB"
                     /locus_tag="QBF46_002582"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(16146..17645)
                     /gene="feaB"
                     /locus_tag="QBF46_002582"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDQ6674748.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            17881..18786
                     /gene="feaR"
                     /locus_tag="QBF46_002583"
     CDS             17881..18786
                     /gene="feaR"
                     /locus_tag="QBF46_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDQ6674749.1"
                     /translation="MNPAMDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(18958..19284)
                     /locus_tag="QBF46_002584"
     CDS             complement(18958..19284)
                     /locus_tag="QBF46_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDQ6674750.1"
                     /translation="MKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSADDIAKLAGQKLVARAKPGEYVQGING
                     KWMRKS"
     gene            complement(19292..19477)
                     /locus_tag="QBF46_002585"
     CDS             complement(19292..19477)
                     /locus_tag="QBF46_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDQ6674751.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(19474..22113)
                     /locus_tag="QBF46_002586"
     CDS             complement(19474..22113)
                     /locus_tag="QBF46_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDQ6674752.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLEGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGDLYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGISRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            22321..23310
                     /gene="ldhA"
                     /locus_tag="QBF46_002587"
     CDS             22321..23310
                     /gene="ldhA"
                     /locus_tag="QBF46_002587"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDQ6674753.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            23421..23843
                     /gene="hslJ"
                     /locus_tag="QBF46_002588"
     CDS             23421..23843
                     /gene="hslJ"
                     /locus_tag="QBF46_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDQ6674754.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(23840..24106)
                     /locus_tag="QBF46_002589"
     CDS             complement(23840..24106)
                     /locus_tag="QBF46_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDQ6674755.1"
                     /translation="MRAAFWVGCAALLLSGCSSEPVQQATAAHVAPGLKVSMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            24380..27904
                     /gene="nifJ"
                     /locus_tag="QBF46_002590"
     CDS             24380..27904
                     /gene="nifJ"
                     /locus_tag="QBF46_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDQ6674756.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRTRALNPEHPVIRGTSANP
                     DTYFQSGEATNPWYNAVYDHVEQAMNDFAVATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     LFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRRKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         6822 a         7535 c         6891 g         6780 t
ORIGIN      
        1 tatatgatga aaaaacggta tgaattaata ctcaaataaa taaagcagag cttaaacata
       61 actctgcttt ataattaaag ggacaaaata attttacatc tgttactgtt tcggtcgcag
      121 attctcatca aataccaggc gtttcctgcc ggattcaact tcccgcaatg gctcaacttg
      181 atgcaacgtc tgcttacagc gtgtcaccag cacctgatac tcatgcgtac cctgcttttt
      241 ccatgccagc tcctgctcac taagttcacg aatcgctttg gccgggctgc cgacaatcag
      301 gtaattagca ggcatttctg ctttggcttt cacaaatgcg gatgcgccaa caatgctgtt
      361 ctcgccaatc accgcaccgt ccatcactac cgcgttcatt cccactaatg cattgcggcg
      421 gataatgcag ctgtgaagga tagcgctatg accaatatgc ccatcttctt ccacgacagt
      481 atcctgctcg ggaaaaccgt gcataacgca attatcctga atgttcgcgc catctttcac
      541 cacgatacga ccaaaatcgc cacgcaggct ggcattaggc ccaacgtaaa cgcccttgcc
      601 gagaataaca tcgccgatca ataccgctgt cggatggaca aagctctctt ctggcacaac
      661 cggagtcaga ccatctatct ggtaaattgg cataacgcct cctgttcaat attcaagccg
      721 ccaaaacgtt gaaaatacag gcttcccggc gcaggcagtt caccgactga ggtttcacct
      781 ttttcactaa cgaacgctaa agcagcaggc gctacgcgct gataaatgtt gatacacagc
      841 tgacgcgccg tatgccctgc ccagtgagcc ggaagcaact cctccggcaa caatgggtct
      901 ttaaggacga cacggcgata aaaatggatc agtaaaagct gaatatgaaa tgcacgctcc
      961 ggggttaact cgtctgccgt ctcttttaac agcggcacca gcgggcggaa tgactgaata
     1021 aaggtttcgt acatggcatt ttgttcagtt aaatgccagc actcttctac tctggcacgc
     1081 agtgctgcgc gagaaagcgc cagtggtatt tgcgcttcaa aacaaatcac gttatccgcc
     1141 acccccgctt catgcaaaag tgtctgtaca tccgccagtt tttgcgacgg cgatgccatc
     1201 aggctgggtg ccagtgcgcc aaaaccttgc cagatcaact gctttttgac atcagccagc
     1261 gtcgctttat ctaaaccttc cgagagcaac aggagccatt taccatccca tgcaggttgc
     1321 tctgcgcgat aaattttact ttctgcccgt cgcgtcaggc gcaagccttt atcactgagg
     1381 ctatagaaac tgcgtcgccc gatgcgggaa acatccagcc agccttcttt attaagacga
     1441 aacaaagcgg tgcgcacgaa acgctcacca aatcccagcc cttccagcaa agcagccaga
     1501 ctaccaagcc agatttcacc accacgatgg gaaagcgaat caccatacag agaggagatc
     1561 aaagatgtgc cactgaccgg aacagcgttt acagcatgtt ggataaaagt atcaagttta
     1621 ctcattcgat tcagtctgtt gttattgttg tgatttatga atcatagcac aggtcgtatc
     1681 gacttctggc cctgacgctt tacctcacca gggccagacg acacgtcagg caccaacaat
     1741 attgcgcaga tcaaacaccc gacacgcctt gccttctgaa cgcgggatac tgccacagtt
     1801 aacgatcatc acatcggtag agatccccac catcgactta atccgatggc gcagctggtg
     1861 gcatacctgg caacgctgct catgcgtcaa tgtcaggcta ctttctttca actccacttt
     1921 cacagaaagt gaatcaagat gcccccggcg gttcacctcc agttggtaat gcggcgataa
     1981 atgttcgaat ttgacaatct cttcttccag ttgtgacgga aagacattta caccgcgaat
     2041 aatcaacatg tcatcgctgc gtccgctgat gcgatccatg cggcgcatag tccgcgccgt
     2101 tcctggtaac agtcgggtta aatcacgcgt gcggtaacga atgaccggca atgcttcttt
     2161 ggtcagcgtg gtgaataaca gttcgccatg ttcaccatcg gcaagcggtg tgccgtcatg
     2221 aggattaaca atttcaggat agaaatgatc ttcccaaatg gttgggccgt cggtagtttc
     2281 cagacactcc atcgccaccc ccggccccat cacttctgac aggccataaa tatccagtgc
     2341 ggtgatcccc aggcgacgct caatttcttt acgcatggcc tgtgtccacg gctcagcacc
     2401 aaacactcca acccgcagcg agcaaccgct ggcatcaccg cccaactgcc gctccagctc
     2461 ttcaatcagg ttaaggcaat aagatggcgt aaccatgatc atatctggtt gaaaatcacg
     2521 gatcagttgc gcttgttttt ctgtctgccc gccagacatc gggatcaccg tagcgcccag
     2581 acgttcggca ccatagtgcg cacccagccc accagtaaac agcccgtaac cataggcgac
     2641 atgaattttg tctttgggcg tgccacccgc cgcacgcaac gaacgcgcta caatattggc
     2701 ccagttatca atatcgtttt gcgtatagcc gacaactgtc ggtttacctg tggttcccga
     2761 agaagcatga atgcgcacca cttgttccat cggcacggca aaggtgtcga aaggatagtt
     2821 gtcgcgcaga tcctgtttgg tggtacaggg aaatttacgc aggtctgaaa gttccctgaa
     2881 atcatcagga tgtacgcctg ctgcgttgaa tttgcgccga tacatcggga cattttcata
     2941 cgcgtgtttg agcgtccatt tcagacgctg tgtttgtaac gcctgtaact catcaacaga
     3001 cgcggtttca atcgggtcaa gctttgtatt ggttatcatt gtagggtact cgcaggttga
     3061 tatgctcaaa cacgctccag aatcatggcg atgccctgac cgacaccgat gcacatggtg
     3121 cacaatgcgt aacgaccgtt acgccgatgc agctcatggc tggcagccag tgccaggcgg
     3181 gcaccactca ttcccaacgg atggcctaag gcgatagcgc ctccgttggg gttaacatgt
     3241 ggggcatcat caggcagccc caattcgcgt agtacaccca acgcctgggc cgcgaatgct
     3301 tcgttcagtt caatcacgtc catatcgtga atactcagcc ccgcgcgttc cagcacccgg
     3361 cgagttgcag gcaccggacc aagccccatc aggcgcggtt ccaccccggc ggttgccatg
     3421 gctacgatac gcgctcgcgg tgtcagtcct tgcgctgctg ccatctgttc actggcaata
     3481 atcaacgcag cggctccgtc attcaccccg gaggcattgc ctgcggtgat cactccattg
     3541 gcacgaaatg gtgcttttag tccacgtaac tgttccagcg tcgtttccgg gcgcaaatgc
     3601 tcgtcatgtt gtatttctgt tacaacacct ttcttatttt tcaacacaac cggaacaatc
     3661 tcttcagcta aaatgcctga agattgcgct tttgccgaac gttgctgact gcgtagcgca
     3721 aaactatctt gatcttctcg cgagattttt aacagttcag ctacattctc tgccgtttcc
     3781 ggcatgctgt cagttccaaa ttgttgagcc atgagcgggt tcacaaatcg ccagccaata
     3841 gtggtatcaa acatctcagc ctgacgagaa aatgcactgg ttgccttgcc cataacaaac
     3901 ggtgcccgag acattgactc cacgccaccg gcgatcagca aatcgccatc gcccgcttta
     3961 atcgcccgtg cggcgaaccc cagtgcgtcc agcccggaac cacacaagcg gttaatggtt
     4021 gtgccggaaa cactctgcgg cagccccgcc agtaaagtcg ccatccgggc tacgttacgg
     4081 ttatcttctc ccgcctgatt agcacagccg aggatcacat catcgataca ctccgcatcg
     4141 agacgcgggt ttcgcaccag cagctcccgc aaagggatag cagccagatc gtcagcccga
     4201 acacctgata atgccccgcc gtagcgacca attggcgtac gaattccgtc acagataaag
     4261 gcttcacgca tcaggcttct cctgtaatgg tgccgccgat gcggtgagat ttaccgcgaa
     4321 acagcgcaac cgttttttgt tgttggttaa caatttcgat gtcgtaaaca ccggtttgct
     4381 tgccctgatg acgtacctgc gcagtagcag ttaaggtgtc tccggcaaag cctggacgta
     4441 aaaaatcaat cgtgcaggca gaagcgacgg ctgccagccc ctggctattg caggcgtagg
     4501 caaaggcagt atcagccagt gaaaatagct gcccgccgtg gcaactttga tgaccgttaa
     4561 gcatttgtgc agtgacggtc atggtcacta cagcaaagcc ttcatccatt gagataatgt
     4621 cgatgccaag cgctttggcg caggcatcgt tctcatacat tgcatgggca ttttgccagg
     4681 ccttatgact cataaccgct ctccagaagc gcccgttggc gcagcaatga acatgggcga
     4741 tagcgttctt cgccatagtg atgctgtaga ttttcaagga gccttaatat tcgctgccag
     4801 ccaagttgcg ctccccaggc aagtgggcca tatggataat tcaccccaag acgcatggcg
     4861 gtatcgatat cctgttcaga ggccacgcct ttttgaagcg catcaagggc ttcattgatg
     4921 atcattgcta ccgttcgcca aatcagcatt cctgggtaat ctgcaatttg cagcactgtt
     4981 ttgccctgct gttgcaggta ataaatggcc ttgcgggtcg ctgagtccgg gttcaccgct
     5041 gccgcagcaa tggtcaccac cttgcccgcc attttgtcga ccactaccac cgggcgtgcc
     5101 agtcgtattg ccagcgcctg tgccgtctcg ccttgtgtct caatcaataa aacatcgtca
     5161 atttccgtga caccgtcact tttcttttct actttcattg ggctaaaact gtcgcttacc
     5221 gcttccaggc caacaactgc ctcacgttcc gcgcgccagt cgtacacgcc cagcccactt
     5281 ttcttgccca accgtccacc aatcaccagt tcctgttgca ccagcgaagg taaaaaacga
     5341 cgctcctgcc agaaagcgtt aaacaccgaa caggtgacag caaaattgac gtcctgacca
     5401 atcagatcgg ttaattccag cggccccatc gggaaaccag cgccatcgcg aagtgcagcg
     5461 tcaatcactt ctggtgcagc aacctgctct tccagtgccc gccaggcctc ggaataataa
     5521 ggacgcgcaa cacggttaac gataaatccg ggggtcgaat gacagcgcac tggctgctta
     5581 ccccaactca atgtcagttc acacaactgc tcgacaactt ccgctgccgt tgccagccca
     5641 ctgaccacct ccaccaactt catcaccggt gccgggttaa aaaaatgcag ccccgcaaca
     5701 cgttcaggat tttttacctc cgcagcaatc gcggttatag agattgacga agtgttagtg
     5761 gtcaatagcg tttgtggcgg gcaaacttcc gccagttgtt caaagagcgc ttttttaact
     5821 tccagacgtt cagacgccgc ttcaataacc agattcgctg ctgccagcgc gtgaatatcg
     5881 gtcaccggga tcaggcgttt caatgtgcgt tcacaggttt cagcggtcag ttttccccgc
     5941 gtcacgcgtg aatttagccg cgcgtgtatc ccgtcgattg cgcgggtcag cgcttcagca
     6001 gaaatgtcat acagtaaaac ctgatgtcca tgactggcgg caacttcagc aatgcctgcc
     6061 cccatggtgc cgctcccaat cactgccaca gtttgcacat ttatcatcat gctatttccc
     6121 cgtgaactgc ggtgagcgtt tagccaggaa cgcactgaca ccttcacgat aatctgcgct
     6181 ccgtccggca agtcgctgat agtcacgttc cagatccagt tgcgtatcga gcgtattggt
     6241 ttcagcgcta tttatcgctt gcttgataag tccaagacca aatgtcggtt gtgtcgccag
     6301 atgccgtgcc agctgttgcg cggtatctgc cagcgtttca tcatcaacaa cctgccagat
     6361 catcccccat tcgtgcgcct gttcagcact cagttgattc cccagcagtg ccagccccat
     6421 ggcgcgcgct cgtccggcaa cgcgtggcag taaccaggtt ccaccgcaat cgggtattaa
     6481 gcctaactta ctaaacgcca tgacgaattt tgctgaacgg gcagcaataa cgatgtcgca
     6541 ccccagtgcc agtgttgcgc ctgcccctgc cgccacgcca ttgactgcac agatcaccgg
     6601 ttttggcagt tttgccaggc gacgtaccag tgggttatag aaacgttcaa ctgacatccc
     6661 taaatccggt gcggggcctg tgggatcaac gttacgatcg ttaagatcct gaccagcaca
     6721 aaatccgcgc ccggcacctg taagtaacag gcaacggata gtgtcgtcgc gctcgacctg
     6781 tttcaggcac tctgccagtt gtgcgtgcat ctcatcatta aaactgttca ggcgttccgg
     6841 gcggttgagc gttagtgtca tcacgccctt ttctacatga ctgaggatga attccatcat
     6901 tagcgtcctt taaagtcggg cgtgcgtttt tgtaagaaag cggagatgcc ttcatgacga
     6961 tcttctgttg ccgccagcaa ggtgaataac tgtcgctcct gggcaagtcc ggcttgcaaa
     7021 gccacttcct gcgactggcg cagcgcttgc tttgccgctt gtaaggccag cggcgagtga
     7081 cgtgccattt tcgatgccag ctgtaaggcg tattcgaggg ttaaatcgct ggggaagacg
     7141 tcgctaacca gcccggcctg ctgtgcttgc tgggcggtga tactttctcc gctcagcacc
     7201 attttgctgg ctaacgattt accgacacta cggattaaac gttgcgttcc gcctgcgcca
     7261 ggcatgatgc cgagagtgat ttccggcaac ccaaaacgcg cgttctctcc ggcaaccacc
     7321 acatcgcaca acaatgcaag ttcgcaaccc gccccaagcg cgtaaccatt gacggctgcg
     7381 ataagtggtt tattaaaggc ctgcaatcgc gcccatagct gcggacgtgt atcgtttaag
     7441 gtggccgcga gatctttttc tgccatttcg ttgagatcgg ccccggcggc aaaaaagcgt
     7501 gcattaccgg taatcacaca gaccgaaatg ctgctatcgg tagccgcagc ttccagttca
     7561 tttaccagtt gcgtcagaag ggcattattt agcgcattac gtgcggcggg acggttaagg
     7621 gtcagcaaca atactcgttg ctgacggctg acgatcagtt cgctcatgcc atccccttcg
     7681 cgtcaaagtc aaccaccaca tcgctggtca gcggcagtgc ctggcaactc aacacatagc
     7741 ctgcggccag ttcatccggt tccagactgt aattggtttc catcgccact ttgccacgca
     7801 gcactttgca tttgcaggtc gcacagacgc cgcctttgca ggcatagggc agatccgccc
     7861 cctggcgcaa tgccgcatcg agaatgcttt catcgtcggc attaagcacg atttcccgat
     7921 cccgcccatc ctgacgtaca gtcacttttt gtccgtcact ttgcacgtta acgctacgtt
     7981 tgacgcgcgt gccaggcgta ttaaaccgct ccagatgaat cgctttttct ggcattccca
     8041 gtgcttttaa ggcggtttcc gcgtcatcca tcatcgctgc cggaccacaa ataaacgcct
     8101 catcataaag acgaaaatta atgagcgagg ccccaagtga ctgtaatttt tcaccgtcaa
     8161 tacgcccgtg aagcagatcg ctgtcgagag tttcctgact gaaaatgcac aacaactgta
     8221 aacgctgagg atatttgtct ttcaggtctg ccagtgcctg gcgaaacatc atgctctggc
     8281 tggtacggtt accgtagatc agggtgaact gactttcagg ctcggtttgt aaagtggtgg
     8341 caataatcgc cagcattggc gtaatacctg atcctgctgc aattgccaga tagcgcccct
     8401 ggcgttcggc ctgcggctga tagccgaaat gcccctgcgg caccatgacc tccagcgtca
     8461 taccctggcg gatgtgttcg cgggcatagc gggagaaacg tccgccttca atggctttca
     8521 ccgccacact aatttcgcca ggcagatagc tgcggcaaat ggagtaacaa cggcgtaatt
     8581 cttcaccatc aaagctggct tttaaggtca aatgttgacc ggggcgaaag cgatacgcct
     8641 cctgcaaagg ctggggcacc gcaaaggtaa tggtcaccgc atcacgggtt tccgactcga
     8701 cttttgccac tgttaaggaa tgaaacgttg tcatggcatc ctcaaataca tttgaaataa
     8761 tcgaaaggtt cgcggcaact atcgcagcgg tacaatgctt tgcaggccgt ggaaccaaat
     8821 tcactgataa gtgtggtatg gacgctggcg cagcgcgggc aacgtacttc tggcggcaaa
     8881 tgggcatggc aacagtgtcc ggcgggcggg ctaatgccat actctcgcag acgctcacgg
     8941 gcatcggggg tcatccagtc ggtggtccat gccgggtcga gttgcagcac aacctgaacg
     9001 ggggtaaacc cgttggttgt cattgcctca cgtatcgcgc caatcaaatg ttccgttgcc
     9061 ggacaaccgg aatatgtcgg cgtaaagccg atcacccatc cttctcccat ctgtgtcaca
     9121 ttacgcacca tgcctaaatc agtaatggtc agcaccggga tctccgggtc cgggatctgg
     9181 ctgagtagcg cccatatctc atgaacctgt ggcggtgcaa tggtggccag acgttgcata
     9241 cccatctcct ctgttaccat tgctgaccgg gcaagacacg ctggagatac tgcatttctg
     9301 ccagcatggg tccaaggtgt tcggtatgca gtcctttttt accgccagtg cgatacgcct
     9361 gctcttgcgg tacgttcaat gtggcttcgt tgatcccggc aaaaacttcg gcttcccacg
     9421 ctgcgcgtaa agtgcgtgga tcaaccgcaa taccctcttc actcagtgca atatcaatct
     9481 cgtcggcatc gaacagttcg gcggtaaaac gccacaactt gttgatggcc tgctgcatct
     9541 tttgccctga tacgtcagta ccattgccca gtcgctccag ccagccacga ctaaaacgca
     9601 ggtgatagcg cgcttcttta attgctttgg cagaaatcgc cgccagttgc ggatcacggc
     9661 tttccatcag acgggtaaag agcgccacat gccatgcatc gatgaaatac tggcgtgcaa
     9721 tggtgtcggc aaaattgccg tttggttgtt caaccaacaa taagttgctg aactggcgct
     9781 cgtctcgggt gaaggccagg gtatcttcat cgccttctcc cgctaattca gcggcatacg
     9841 ataagaagtt gcgtgcctga cctaataaat cgaggccaat gtttgccagt gcgagatcga
     9901 tttccagttc cggtgcgtga ccgcaccatt cacccaaccg ctgggagagc accaggcagt
     9961 tatcgcccag gcgcaaggtg taagccgtta actgattcat ttccgacctc acatgtgctc
    10021 aatgccatca gggatggtgt aaaacgttgg atggcgatag accttgcttt cagccggatc
    10081 aaaaaattca ccgcgttctt ccggttgcga ggcaacaatt tcactcgcct tcaccaccca
    10141 aattgaacat ccttcgctac gacgggtgta agcatcacgg gcattttcca gtgccatccg
    10201 ctcatcggca gcatgtaaac tgccaacatg gcggtgtgat aagccctgtt tgccacgcac
    10261 gaaaacttcg tataacggcc agtaaacatt actcattttg aatctcctta tgcgacctta
    10321 cgggcatgtt gtttttgtgc atgggccagc gctgcttccc gtacccaggt accttcttcc
    10381 caggctttac gtttggcgtc gagcctctcc tgattacaaa ttccgcgacc gttaattact
    10441 tcgttaaact cctgccagtc gatctcacca aagcggtagt gaccgctttc agtgtcaaaa
    10501 tgcagatccg ggtcaggaac ggtcataccg agcatttcaa cctgtggaac ggtgttatcc
    10561 acgaaacgct ggcggagttc gtcgttggtg aaacgtttga ttttccaggt gagacttctg
    10621 gcgctgtttg gcgagttatc atcgtttggc ccgaacatca ttaaggctgg ccaccagaaa
    10681 cggttaatgg cgtcttgcaa catctgcttt tgagcttcgc tgccctgcgc cagtgccatg
    10741 caggcttcaa acccctgacg ctggtgaaaa ctctcttctt tgcagatttt caccattgct
    10801 ctggcatacg ggccataaga agtgcggcat aacgccacct gattgacgat cgctgcgcca
    10861 tccaccagcc agccgataac accgatatca gcccaactca atgtcggata attaaagatg
    10921 gaggaatatt tcatccgtcc gtcgagcatt ttttggtaga tgtcttctct ggcgcagccc
    10981 agtgtttcgg cggcgctata gagatacaga ccatgaccgg cttcatcctg cactttggcc
    11041 aacagaatgg ctttacgccg caaggttggt gcgcgagtga tccagttacc ttcaggcaac
    11101 ataccaacaa tctcggagtg cgcatgctgc ccaatctggc ggatcaatgt ctttcggtaa
    11161 gcatcgggca tccagtcctg tggctcgata gccgtttcct gggctatccg ttgctcaaag
    11221 cgttcttctt gggtcacgtc atcaccttta cgattccagt tttcgtgaat taattaaaaa
    11281 cacgaatcac attgttttat gaaagttaca caatagttaa ctaaaatcac aaattttgta
    11341 tgtttgaact gtgacggatt tcgcacccta tttttataaa tcattattta tcagtgcatt
    11401 aatgttttcc gcagttgcta tacagatcgc atagttaaca tttcgttaaa agatcccttg
    11461 ctttttatga ttcgcgattt aactattagc aacagaaatg tgaaacatct ggagagtagc
    11521 gatgcagcag ttagccagtt tcttatccgg tacctggcag tctggccggg gccgtagccg
    11581 tttgattcac cacgctatta gcggcgaggc gttatgggaa gtgaccagtg aaggtcttga
    11641 tatggcggct gcccgccagt ttgccattga aaaaggtgcc cccgcccttc gcgctatgac
    11701 ctttatcgaa cgtgcggcga tgcttaaagc ggtcgctaaa catctgctga gtgaaaaaga
    11761 gcgtttctat gctctttctg cgcaaacagg cgcaacgcgg gcagacagtt gggttgatat
    11821 tgaaggtggc attgggacgt tatttactta cgccagcctc ggtagccggg agctgcctga
    11881 cgatacgctg tggccggaag atgaattgat ccccttatcg aaagaaggtg gatttgccgc
    11941 gcgccattta ctgacctcaa agtcaggcgt ggcagtgcat attaacgcct ttaacttccc
    12001 ctgctgggga atgctggaaa agctggcacc aacgtggctg ggcggaatgc cagccatcat
    12061 caaaccagct accgcgacgg cccaactgac tcaggcgatg gtgaaatcaa ttgtcgatag
    12121 tggtcttgtt cccgaaggcg caattagtct gatctgcggt agtgctggcg acttgttgga
    12181 tcatctggac agccaggatg tggtgacttt cacggggtca gcggcgaccg gacagatgct
    12241 gcgagttcag ccaaatatcg tcgccaaatc tatccccttc actatggaag ctgattccct
    12301 gaactgctgc gtactgggcg aagatgtcac cccggatcaa ccggagtttg cgctgtttat
    12361 tcgtgaagtt gtgcgtgaga tgaccacaaa agccgggcaa aaatgtacgg caatccggcg
    12421 gattattgtg ccgcaggcat tggttaatgc tgtcagtgat gctctggttg cgcgattaca
    12481 gaaagtcgtg gtcggtgatc ctgctcagga aggcgtgaaa atgggcgcac tggtaaatgc
    12541 tgagcagcgt gccgatgtgc aggaaaaagt gaacatattg ctggctgcag gatgcgagat
    12601 tcgcctcggt ggtcaggcgg atttatctgc tgcgggtgcc ttcttcccgc caaccttatt
    12661 gtactgtccg cagccggatg aaacaccggc ggtacatgca acagaagcct ttggccctgt
    12721 cgcaacgctg atgccagcac aaaaccagcg acatgctctg caactggctt gtgcaggcgg
    12781 cggtagcctt gcgggaacgc tggtgacggc tgatccgcaa attgcgcgtc agtttattgc
    12841 cgacgcggca cgtacgcatg ggcgaattca gatcctcaat gaagagtcgg caaaagaatc
    12901 caccgggcat ggctccccac tgccacaact ggtacatggt gggcctggtc gcgcaggagg
    12961 cggtgaagaa ttaggcggtt tacgagcggt gaaacattac atgcagcgaa ccgctgttca
    13021 gggtagtccg acgatgcttg ccgctatcag taaacagtgg gtgcgcggtg cgaaagtcga
    13081 agaagatcgt attcatccgt tccgcaaata ttttgaggag ctacaaccag gcgacagcct
    13141 gttgactccc cgccgcacaa tgacagaggc cgatattgtt aactttgctt gcctcagcgg
    13201 cgatcatttc tatgcacata tggataagat tgctgctgcc gaatctattt tcggtgagcg
    13261 ggtggtgcat gggtattttg tgctttctgc ggctgcgggt ctgtttgtcg atgccggtgt
    13321 cggtccggtc attgctaact acgggctgga aagcttgcgt tttatcgaac ccgtaaagcc
    13381 aggcgatacc atccaggtgc gtctcacctg taagcgcaag acgctgaaaa aacagcgtag
    13441 cgcagaagaa aaaccaacag gtgtggtgga atgggctgta gaggtattca atcagcatca
    13501 aaccccggtg gcgctgtatt caattctgac gctggtggcc aggcagcacg gtgattttgt
    13561 cgattaatcg gtgaatgaag ggcaacggcg aatagttgcc cttttatttc actaagtttt
    13621 gtgacgttgt cacattatgc atgatgtgta catctatttt cagggcatcc actgtatgaa
    13681 aagctggcac acctgccaaa caacctggca ggtgcaggca atcccctttg catcagtact
    13741 gataatgtga acctgactaa accgcccaca gagcgcggtt gctaacaaga acacaacatc
    13801 tgacgaggtt aataatggga agcccctctc tgtattctgc ccgtaaaaca accctggcgt
    13861 tggcagtcgc cttaagtttc gcctggcaag cgccggtatt tgcccacggt ggtgaagcgc
    13921 atatggtgcc aatggataaa acgcttaaag aatttggtgc cgatgtgcag tgggacgact
    13981 acgcccagct ctttaccctg attaaagatg gcgcgtacgt gaaagtgaag cctggtgcgc
    14041 aaacagcaat tgttaatggt cagcctctgg cactgcaagt accggtagtg atgaaagaca
    14101 ataaagcctg ggtttctgac acctttatta acgatgtttt ccagtccggg ctggatcaaa
    14161 cctttcaggt agaaaagcgc cctcacccac ttaatgcgct aactgcggtc gaaattaaac
    14221 aggccgttga aattgttaaa gcttccgcgg acttcaaacc caatacccgt tttactgaga
    14281 tctccctgct accgccagat aaagaagctg tctgggcgtt tgcgctggaa aacaaaccgg
    14341 ttgaccagcc acgcaaagcc gacgtcatta tgctcgacgg caaacatatc atcgaagcgg
    14401 tggtggatct gcaaaacaac aaactgctct cctggcaacc cattaaagac gcccacggta
    14461 tggtgttgct ggatgatttc gccagtgtgc agaacattat taacaacagt gaagaatttg
    14521 ccgctgccgt gaagaaacgc ggtattactg atgcgaaaaa agtgattacc acgccgctga
    14581 ccgtaggtta tttcgatggt aaagatggcc tgaaacaaga tgcccggttg ctcaaagtca
    14641 tcagctatct tgatgtcggt gatggcaact actgggcaca tcccatcgaa aacctggtgg
    14701 cggtcgttga tttagaacag aaaaaaatcg ttaagattga agaaggtccg gtagttccgg
    14761 tgccaatgac cgcacgccca tttgatggcc gtgaccgcgt tgctccggca gttaagccta
    14821 tgcaaatcat tgagcctgaa ggtaaaaatt acaccattac tggcgatatg attcactggc
    14881 ggaactggga ttttcacctc agcatgaact ctcgcgtcgg gccgatgatc tccaccgtga
    14941 cttataacga caatggcacc aaacgcaaag tcatgtacga aggttctctc ggcggcatga
    15001 ttgtgcctta cggtgatcct gatattggct ggtactttaa agcgtatctg gactctggtg
    15061 actacggtat gggcacgcta acctcaccaa ttgctcgtgg taaagatgcc ccgtctaacg
    15121 cagtgctcct taatgaaacc atcgccgact acactggcgt gccgatggag atccctcgcg
    15181 ctatcgcggt atttgaacgt tatgccgggc cggagtataa gcatcaggaa atgggccagc
    15241 ccaacgtcag taccgaacgc cgggagttag tggtgcgctg gatcagtaca gtgggtaact
    15301 atgactacat ttttgactgg atcttccatg aaaacggcac tattggcatc gatgccggtg
    15361 ctacgggcat cgaagcggtg aaaggtgtta aagcgaaaac catgcacgat gagacggcga
    15421 aagatgacac gcgctacggc acgcttatcg atcacaatat cgtgggtact acacaccaac
    15481 atatttataa tttccgcctc gatctggatg tagatggcga gaataacagc ctggtggcga
    15541 tggacccagt ggtaaaaccg aatactgccg gtggcccacg caccagtacc atgcaagtta
    15601 atcagtacaa catcggcaat gaacaggatg ccgcacagaa atttgatccg ggcacgattc
    15661 gtctgttgag taacccgaac aaagagaacc gcatgggcaa tccggtttcc tatcaaatta
    15721 ttccttatgc aggtggtact cacccggtag caaaaggtgc ccagttcgcg ccggacgagt
    15781 ggatctatca tcgtttaagc tttatggaca agcagctctg ggtaacgcgt tatcatcctg
    15841 gcgagcgttt cccggaaggc aaatatccga accgttctac tcatgacacc ggtcttggac
    15901 aatacagtaa agataacgag tcgctggaca acaccgacgc cgttgtctgg atgaccaccg
    15961 gcaccacaca tgtggcccgc gccgaagagt ggccgattat gccgaccgaa tgggtacata
    16021 ctctgctgaa accatggaac ttctttgacg aaacgccaac gctaggggcg ctgaagaaag
    16081 ataagtgatt gtttcagaca aaaaaacggc accaggtgcc gttttttact tatgagcgaa
    16141 ccagattaat accgtacaca caccgactta gtttcacacc aaccgtccag ccagtcgggg
    16201 ccaaaatcac ggcccgttcc tgactgcttc atcccaccaa acggtaagtt agcgtcaatt
    16261 aaggtatggc tgtttaccca caccgtccct gcctgtaagc gatcgctata ttccagagcc
    16321 tgggagagat tttgcgtcca gacactggca gttaagccat attccgtgtc gtttgccagt
    16381 tgtaacgcct cttctccatc cgctactcgc accaggttta ccaccggacc aaacacctct
    16441 tcacgagtta agcgcaattt agcatcggga tttaccacca gcgttggcgc aacataatac
    16501 ccctctccgg ctggtccatt cgacccgcga atcagctctg cttgctgtgc ctgcgcatcg
    16561 tcgaggaatg aacacacttt gtcgcagtgc gcacgagaaa ccaaagggtt aatctgtgca
    16621 acaggtgaca tccccggtcc cacttgcaac gattttaccg cctgctcaaa tccactaacc
    16681 agcgtgtcaa acaacggcgc ttcaatataa attcgcgaac tggcggcgca tacttgccct
    16741 tgattcagga agcttccggt catcaagcct tcaataaccc attgcggatc agcatctttt
    16801 aatacaattg ccgggttttt accgcccagt tccagcgtta cacgcgttaa gtgatcagca
    16861 gcagttctgg caataccttt tcccgtcgcg gttgaaccgg taaaactgat tttcgcaaca
    16921 tgaggatgtg acgtcagggc cgcgccgcat acagcacctg acccggtgac gacattaaaa
    16981 acgccatcag ggataccagc ctcgctggcc agttccgcca cgcgcaacat cgtcagtggc
    17041 gtggtttccg aaggcttaat cacgattgaa cagcctgctg ccagtgctgg catcaccttc
    17101 cacataccaa tcatcaacgg aaagttccat ggcacaattc ccgccactac gccaaccggc
    17161 tctttacgcg tccaggcctg ataacgcgcc ccctggggta agggaatcga caagtccagc
    17221 gttttacccg cgattttggt cgttaacccg gcggtataac gcatccagtt cagcgtacag
    17281 cccacttcaa aagcacggga aatggcaatt gacttgcctt gctccagggt ttccagttgc
    17341 gccagctcct cactgtgctg ttccaccaga tcggcaaaac gtagcagaat acgttcacgc
    17401 tctgcgggta atcgcccggc ccagcgacgc gagacaaagg cccgccaggc agacatgact
    17461 gcgttatcta catccgcttc attggcatca gcagtagacg caatttcttg cccggtggcc
    17521 ggatcaaaga tcgccaatcg tttttcactt tgtgcggggc ctggacgacc atcaatataa
    17581 agaccgtgtt gacgatcgag aaactgttgg acctggctta atactgctac atgcggctct
    17641 gtcatcactt ttccttatta tttacccagt gtgatttcag tgtacgcagc gacaagaaaa
    17701 gtcacttttt tcgctgtgac attcgctttg tcagctgtga caagctccgc aaatcgtgac
    17761 aataaccgct tatgtctcta tgcctgagaa gacttatttc ctatagtcag gctcattagc
    17821 ttcgtttatt gcaacacaaa tgcaacaata aaaatacatt tcacagagcg aaaacgtgcc
    17881 atgaaccccg caatggataa tgagtttcag caatggcttt cccaaatcaa tcaggtatgc
    17941 ggaaatttta ccggacgcct gctgactgag cgttacacgg gtgtactgga cacgcatttt
    18001 gccaaaggac taaagctgag taccgtgaca accagcgggg tgaatttatc ccgcacctgg
    18061 caggaggtaa aaggcagcga cgatgcctgg ttttacaccg tttttcagct tagtggtcag
    18121 gcaataatgg agcaggatga gcgtcaggtg cagattggcg ctggcgatat tacgttactc
    18181 gatgcctcac gcccctgttc gctttactgg caggagtctt ctaaacagat ttcattactt
    18241 ttgccacgca ctctgctgga acaatatttt ccccatcaaa aacctgtctg cgcagaaaga
    18301 ctggacgctg acttacccat ggtgcaactc agtcatcgcc tgttacagga gagcatgaat
    18361 aatccggcac tttctgaaac agaaagtgaa gctgcgctac aggcgatggt gtgtctgctg
    18421 cgcccggtac ttcatcagcg ggaatctgtt caacctcgtc gtgaacgtca gtttcaaaaa
    18481 gtggttacgt tgatagacga taatattcgc gaagagatat tacgcccgga gtggatagcc
    18541 ggagagacag gtatgtcagt acgtagtttg taccgaatgt ttgccgataa aggtttggtg
    18601 gtcgcgcaat atattcgtaa ccgtcgtctc gatttttgtg cagatgcgat tcgccatgcc
    18661 gcagatgatg aaaaactggc aggcatcggc tttcattggg gattttctga ccagagtcat
    18721 ttttcaacgg tatttaagca acgctttggg atgacgccag gcgagtatcg acgtaaattc
    18781 cgctaactaa ttcgcgttgc atgtactgca acgccaatta gttaaaacag atatttgact
    18841 acgcatattt cagggcaaaa ggcaatttga caggagtttt tgaatattca ccttgtttcc
    18901 ggaaaataga ttaatcgctt tctcctgtag cgttatcccg atattgcggt tttgctatta
    18961 agattttcgc atccatttac cgtttatccc ttgtacatat tcccccggtt tggcccgagc
    19021 tacgagcttt tgcccggcga gtttcgcgat atcatctgca gacacattat tttgctttgc
    19081 cagttgttgg taactcgctt tacgttcggc attaatgtcg gttactaatt tctctgtttc
    19141 agcatccgtt ttcagcgcaa ccaaataacc ataaaatgtt tcacctactc gcccctgagt
    19201 tctggcttca tccagagtca atgccattac attgctgctt accagcccaa caagaaacgc
    19261 acaaagaagt aatgttttct tcatcatcgc ctcagaaaag atcgctacga gtttcaagca
    19321 gctcttcgac atctttgtct gccttgatga tgatctcatg ctcaatttta acgttcatat
    19381 tgatagtgat cggttcctta ggtgcagcga cttcaatgcg aggcgtacag ccaaccagca
    19441 taaaagatga cgtcaacgca gccagtaaaa ttttcattgt ttttcctcac actcttttcc
    19501 ttgcggacag tcatttcccg gcagacgtgc gttctgctcc agccatgcct ggagattatc
    19561 gccaaagcgt aaactgcgcc acagcgtaaa aatattctct tcatgatggt aattaaggtt
    19621 taccgtaccg cttttaccat caacgcgact gatacctgta atgttggcct gcatggttaa
    19681 taaaccgaga ttatctaaat taatttttgt cgatgaacgg ctaatttcca tatagcgcaa
    19741 ccagttaatt gctgaacccg cagtcatatt gtcttttacc accgcatccg cagtgtcttt
    19801 atccagtcgc aatgtcatcg gcccgctatt cgccagccag ccatctttca caatccattt
    19861 ttcattgttc agccataacg gcagtgcacc actaaatgcc ccggacatgg cgaattgttt
    19921 cggattgacg gcgctaacca gttcgctgga tgagagatta ttcagccgca acagcgccgg
    19981 gtcatgttgc ggcatacgta attgtttcat cagtacgtta ccgcctaaca catcgacgct
    20041 aacatcagtc aacagcaagg gttcttcttc agtccacgga taacgccctt gcaaatcagc
    20101 cgtaatattt ttcgctgtca ccagattaat cacttcggca attcgcaacg taacggggcc
    20161 gcgagtcccc agatgccagg ctccatcggc aaaacggaaa ggcaggacaa aatcgacacc
    20221 gttaacctga ttatctggca tccaggcact accgcctttc aacacgccgt gtcctcccgc
    20281 gcggaatcct tgttcaggcg cagcagaaaa tgcaacctga gcatacagat caccatcgcg
    20341 taagttcatc ttccagtcgg gtggcaccag cggctggaat acagtcagtg attgttttgg
    20401 ccaccaggcg ttaccgcgca gacgaatacc gtcccagcga ccatttaccc gaaccgggcc
    20461 aatctcacca gcatgtaaat cgcctttaaa gaggaaatag gtcggatcgc gcccatcgac
    20521 gctaaatttt aaggttgatg gcggtaacac actgccgcca gtgaacagcg tttgcccggc
    20581 gtccagtgac agcgcgccgc tgaaggaggg atgctgtgcg tcacgtaccc agacgacggg
    20641 cttgtcgaga attagtcgcg gcttttctac cgtcatcgta ccgtattgta actggtcgaa
    20701 accggtggag agatcggtca gtgtaatcgt gctgtcatgc cactcaccct ttcctgcgac
    20761 atcccaggtg gcattcatcg gtgtaaaact gccttttccc cagtagcgcc actgccagcg
    20821 gccagcgtca gggagaaaat cattcgccaa cccatccata tgcagcacga aatcgcccag
    20881 ttcattttca tgcgcctgca aaatggcctg caaacgtccg tcaacaccac gttgggtgac
    20941 ttttacaccc gctaaaggcc agcggatttc atcgatgtcc agcgaatcga tgactcttcc
    21001 cttcgaacgt aataacgcgc ctggctcaaa ggtcagcgtt gggtcagaca gacttccact
    21061 tagctgcgca ggtaaacggg catataaaat gaggtccgcc tgtttcgctt caccggtcag
    21121 ctgcagaggc agctgactgt tatccatgct taattttcct gggccaaaat taagcaccgc
    21181 gttgccttta cccgcttgcc cctgggtcag cacactcagt cgcccgctga ccagagcgtt
    21241 ctcgagccct gcctgccagt tgtcgacttt gacacccagt cggccactca aaggaaaacc
    21301 tgcatacggc caactccagc gaccatcgct tacggtcaat tgttgacgag taatttgcca
    21361 cggcaaatcg agcaacggat cgccgttatc ccgtgccagc acaatcaatt gcccgctatt
    21421 ttcctgccag tccagctctg catccaccag tgacggttcc tgcggcaagt ttaacgtcgc
    21481 agtagcatgc ccacttacag gaagtccatc cggcacgagc ggcatagtaa attcccccac
    21541 cagttttacc ggcggctgat tttcaaacgc ggcgacatcc agttcgctaa ctgtaagttg
    21601 ttgccctttc agctggcctt gaaatttaac tttttcgccc tgataacgca gttgctggat
    21661 atcagaggtt aatgcgagag agagttttcc ctgccattcc tgccagggag aaaaaatcag
    21721 tttatcaata ttgatccagg tgttaggcag catggactgc cactgcgcga gggtttttgg
    21781 agcagctggc gattgctccg tctgcggcaa tttcgccaga caagcagaat caagttctac
    21841 cgtgccgacg ttcaataacc agcggctggg atgtgaaagg ctggcgttgg tgatatgcgc
    21901 aagctgacaa tctccctcca gataacggag atcgggaatg attaaacctt tacgcgtaat
    21961 gcgtgggctt tcatctaatg caatacgtgt cccgagcggt agccagatgc ccgccagcgt
    22021 gggaacccac agcccgagcg tcatcagcag cgtcaacggc acaagaataa tcagtaataa
    22081 cagcgcgaga acggctttat atttacccag catgggtagt taatatcctg atttagcgaa
    22141 aaattaagca ttcaatacgg gtattgtggc atgtttaacc gttcagttga aggttgcgcc
    22201 tacactaagc atagttgttg atgaattttt caatatcgcc atagctttca attaaatttg
    22261 aaattttgta aaatattttt agtagcttaa atgtgattca acatcactgg agaaagtctt
    22321 atgaaactcg ccgtttatag cacaaaacag tacgacaaga agtacctgca acaggtgaac
    22381 gagtcctttg gctttgagct ggaatttttt gactttctgc tgacggaaaa aaccgctaaa
    22441 actgccaatg gctgcgaagc ggtatgtatt ttcgtaaacg atgacggcag ccgcccggtg
    22501 ctggaagagc tgaaaaagca cggcgttaaa tatatcgccc tgcgctgtgc cggtttcaat
    22561 aacgtcgacc ttgacgcggc aaaagaactg gggctgaaag tagtccgtgt tccagcctat
    22621 gatccagagg ccgttgctga acacgccatc ggtatgatga tgacgctgaa ccgccgtatt
    22681 caccgcgcgt atcagcgtac ccgtgacgct aacttctctc tggaaggtct gaccggcttt
    22741 actatgtatg gcaaaacggc aggcgttatc ggtaccggta aaatcggtgt ggcgatgctg
    22801 cgcattctga aaggttttgg tatgcgtctg ctggcgttcg atccgtatcc aagtgcagcg
    22861 gcgctggaac tcggtgtgga gtatgtcgat ctgccaaccc tgttctctga atcagacgtt
    22921 atctctctgc actgcccgct gacaccggaa aactaccatc tgttgaacga agccgccttc
    22981 gatcagatga aaaatggcgt gatgatcgtc aataccagtc gcggtgcatt gattgattct
    23041 caggcagcaa ttgaagcgct gaaaaatcag aaaattggtt cgttgggtat ggacgtgtat
    23101 gagaacgaac gcgatctatt ctttgaagat aaatccaacg acgtgatcca ggatgacgta
    23161 ttccgtcgcc tgtctgcctg ccacaacgtg ctgtttaccg ggcaccaggc attcctgaca
    23221 gcagaagctc tgaccagtat ttctcagact acgctgcaaa acttaagcaa tctggaaaaa
    23281 ggtgaaacct gcccgaacga actggtttaa tcttgccgct cccctgcaat ccaggggagc
    23341 tgattcagat aatccccaat gacctttcat tctctattct taaaatagtc ctgagtcaga
    23401 aactgtaatt gagaaccaca atgaagaaag tagccgcgtt tgttgcgcta agcctgctga
    23461 tggcgggatg tgtaagtaat gacaaaattg ctgttacgcc agaacagcta cagcatcatc
    23521 gctttgtgct ggaaagcgta aacggtaagc ccgtgaccag cgataaaaat ccgccagaaa
    23581 tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg taaccgcttt agcggtgaag
    23641 gcaaactgtc taatggtgaa ctgacagcca aagggctggc aatgacccgt atgatgtgcg
    23701 ctaatccgca gcttaatgaa ctcgataaca ccattagcga aatgctgaaa gaaggtgcac
    23761 aagtggatct gaccgcgaac cagttaacgc tggcgacagc gaaacagaca ttaacttata
    23821 agctggcgga tttaatgaat taatagctgc cacagctccc ggcggcaagt gactgttcgc
    23881 tacagcgttt gccgttgggt aatgcacaca tcccaatcgc cgtaccatcc agttgacggg
    23941 caacagaaag cgaaccgccg atcattgcac aatttgcttc tccactactg gacatcgaca
    24001 cttttaaacc tggcgctacg tgcgccgcag tcgcctgctg aacaggttca ctactacacc
    24061 ccgacaacaa taaagcggca caccctaccc aaaacgctgc tcgcatcttt tcttcctcta
    24121 atcttcaagc caaacgacac cgccataaat aataggcagc acagagggcg gcgtcgagag
    24181 ctgtcctgcg cgttgccccg catttttact tttttatggc tatttttttg ccctctgttt
    24241 gatcaaaaca ttcattacgc tgatgtgggg gacacaaaag cgaaaatgca gaagaaagcc
    24301 atttgctaaa attgaaagat tactactggg cgcgcagcaa tttcgtgcgc ccctcatttg
    24361 cgcaatgtaa gggtgtcata tgattactat tgacggtaat ggcgcggttg cttcggtcgc
    24421 atttcgcacc agtgaagtta tcgccatcta ccctattacc cccagttcca cgatggcaga
    24481 acaggctgat gcctgggccg gaaacggctt aaagaacgtt tggggagaca caccacgcgt
    24541 ggttgaaatg cagtcggaag cgggtgctat cgctaccgtg catggcgctt tgcagacggg
    24601 tgccctttca acatcgttta catcatcgca gggtttgctg ctgatgatcc cgacgctgta
    24661 caaactggca ggcgaactaa caccgtttgt cctgcatgta gcggcacgta ccgttgccac
    24721 acatgcactc tctatttttg gcgatcattc cgacgtcatg gcggtgcgcc agacgggttg
    24781 cgcgatgttg tgtgcggcaa acgtccagga agcgcaagac tttgctctca tttcgcacat
    24841 cgcgacgctg aaaagccgcg tgccatttat tcatttcttt gatggtttcc gcacgtccca
    24901 cgaaatcaat aaaattgtcc cgctggccga tgacactatt cttgatctca tgccgcaggc
    24961 cgaaattgat gctcatcgca cccgggcgct caacccggaa catccggtga tccgcggtac
    25021 gtccgccaat cctgacactt atttccagtc tggcgaagcc accaacccct ggtacaacgc
    25081 ggtctatgac catgttgaac aggcgatgaa tgatttcgct gtcgcgacag gtcgtcagta
    25141 tcagccgttt gaatattacg ggcatccgca agcggaacgg gtgattatcc tgatgggctc
    25201 tgccattggc acctgtgaag aagtggttga tgaattgcta acccgtggcg aaaaagtcgg
    25261 cgtgctgaaa gttcgcctgt accgcccctt ctccgctaaa catttactgc aagctctgcc
    25321 gggatccgta cgcagcgtgg cggtactgga cagaaccaaa gaacccggtg cccaggcaga
    25381 accgctctat ctggatgtga tgaccgcact ggcagaagcc tttaataatg gcgagcgtga
    25441 aaccctgccc cgtgtcattg gtgggcgcta tggtctttca tccaaagaat ttggcccaga
    25501 ctgtgtactg gcggtatttg ccgagctcaa cgcggctaaa ccgaaagcgc gctttacggt
    25561 tggtatttac gatgatgtaa ccaatctgtc cctgccgttg ccggaaaaca ccctgccaaa
    25621 ctcggcgaaa cttgaagcct tgttttatgg ccttggtagt gatggcagcg tttccgcgac
    25681 caaaaacaat atcaagatta tcggtaattc cacgccgtgg tacgcacagg gctattttgt
    25741 ttacgactcc aaaaaagcgg gcggcctgac ggtttctcac cttcgcgtga gcgaacagcc
    25801 gattcgttcc gcttatctca tttcccaggc tgattttgtt ggctgccacc agttgcagtt
    25861 tatcgataaa tatcagatgg ctgagcgttt aaaacctggc ggccttttcc tgctcaacac
    25921 gccgtacagc gcagatgaag tgtggtcgcg cttgccgcag gaagttcagg ccgtgttaaa
    25981 ccagaaaaaa gcgcgcttct atgtgattaa cgcggcgaaa atcgcccgcg aatgtggcct
    26041 ggcggcccgt attaataccg tcatgcagat ggcttttttc catctgacgc aaattctgcc
    26101 tggcgatagc gccctcgcag aattgcaggg tgcgattgcc aaaagttaca gtagcaaagg
    26161 ccaggatctg gtggaacgca actggcaggc tctggcgctg gcgcgtgaat ccgtagaaga
    26221 agttccgttg caaccggtaa atccgcacag cgccaatcga ccgccagtgg tttccgatgc
    26281 cgcccctgat ttcgtgaaaa ccgtaaccgc tgcgatgctc gccgggcttg gtgatgccct
    26341 ccccgtttcg gcgctgccgc cagacggcac ctggccgatg ggcaccactc gctgggaaaa
    26401 acgcaatatc gccgaagaga tccccatctg gaaagaggaa ctctgtaccc aatgtaacca
    26461 ctgcgttgcc gcttgcccac actccgctat tcgcgcaaaa gtggtgccgc ctgaagcgat
    26521 ggaaaacgcc cctgccagcc tgcattcgct ggatgtgaaa tcgcgtgata tgcgcgggca
    26581 gaaatatgtc ttgcaggtgg ctccggaaga ttgcaccggt tgtaacctgt gcgtcgaagt
    26641 ttgcccggcg aaagaccgtc agaatccaga gattaaagcc atcaatatga tgtcgcgcct
    26701 ggaacatgtc gaagaagaga aaatcaatta cgatttcttc ctcaacctgc cagaaatcga
    26761 ccgtagaaaa ctagaacgta ttgatattcg tacatcgcag ctgattacac cgctgtttga
    26821 atattcaggt gcttgctccg gttgtggcga gacgccgtat attaaattgc tgactcagct
    26881 ctatggcgat cggatgttga tcgctaacgc caccggctgt tcttcaattt atggcggtaa
    26941 cctgccctct acaccgtata ccaccgatgc caacggtcgt gggccggcat gggcgaactc
    27001 tctatttgaa gataatgccg aatttggcct tggtttccgc ctgacggtcg atcaacaccg
    27061 tgtccgcgtg ctgcgtctgc tggatcaatt tgccgataaa atcccggcgg aattactgac
    27121 ggcgttgaaa tcagacgcca cgccagaggt tcgtcgtgaa caggttgcag ctttacgcca
    27181 gcaactcaac gatgttgccg aagcacatga actgctacgt gatgcagatg cactggtgga
    27241 aaaatcaatc tggctgattg gtggtgatgg ctgggcttac gatatcggct ttggcggtct
    27301 ggatcatgta ttgagtttga cggaaaacgt caacattctg gtgctggata cgcaatgcta
    27361 ttccaacacc ggtggtcagg cgtcaaaagc gacaccgctg ggtgcagtaa ctaagtttgg
    27421 cgagcacggc aaacgtaaag cgcgtaaaga tcttggcgtc agtatgatga tgtacggtca
    27481 tgtttatgtg gcgcagattt ctctcggcgc gcaactgaac cagacggtga aagcgattca
    27541 ggaagcggaa gcgtatccgg gaccatcgct gatcatcgct tatagcccat gtgaagagca
    27601 tggttacgat ctggcactca gccacgacca gatgcgccaa ctcacagcta ccggcttctg
    27661 gccgctatat cgctttgatc cgcgtcgtgc cgatgaaggc aaactgccgc tggccttgga
    27721 ttcacgcccg ccgtcagaag cactggaaga aacgttactt catgagcaac gtttccgtcg
    27781 tctgaattcg cagcagccag aagtggcaga acagttatgg aaagatgctg cagctgattt
    27841 gcaaaaacgc tatgacttcc tggcacaaat ggccggaaaa gcggaaaaaa gcaacaccga
    27901 ttaaatgctc tggataagga ttatccaatt ctaaaaaaaa agcccggacg actgttcggg
    27961 cttgtctttt tatatgttgg aaaatcagtg gcaatgcaat ggcccaacag aaaataaaat
    28021 ctgtgaca
//
LOCUS       DAOFQD010000060        27965 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.060, whole genome shotgun sequence.
ACCESSION   DAOFQD010000060 DAOFQD010000000
VERSION     DAOFQD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.060"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     repeat_region   19..1207
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19..47
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
     gene            complement(1228..2265)
                     /gene="iap"
                     /locus_tag="QBF46_002591"
     CDS             complement(1228..2265)
                     /gene="iap"
                     /locus_tag="QBF46_002591"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDQ6674757.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2517..3425
                     /gene="cysD"
                     /locus_tag="QBF46_002592"
     CDS             2517..3425
                     /gene="cysD"
                     /locus_tag="QBF46_002592"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDQ6674758.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3427..4854
                     /gene="cysN"
                     /locus_tag="QBF46_002593"
     CDS             3427..4854
                     /gene="cysN"
                     /locus_tag="QBF46_002593"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDQ6674759.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4854..5459
                     /gene="cysC"
                     /locus_tag="QBF46_002594"
     CDS             4854..5459
                     /gene="cysC"
                     /locus_tag="QBF46_002594"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDQ6674760.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5509..5832
                     /locus_tag="QBF46_002595"
     CDS             5509..5832
                     /locus_tag="QBF46_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDQ6674761.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            6026..6337
                     /gene="ftsB"
                     /locus_tag="QBF46_002596"
     CDS             6026..6337
                     /gene="ftsB"
                     /locus_tag="QBF46_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDQ6674762.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KFKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6356..7066
                     /gene="ispD"
                     /locus_tag="QBF46_002597"
     CDS             6356..7066
                     /gene="ispD"
                     /locus_tag="QBF46_002597"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDQ6674763.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAADDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            7066..7545
                     /gene="ispF"
                     /locus_tag="QBF46_002598"
     CDS             7066..7545
                     /gene="ispF"
                     /locus_tag="QBF46_002598"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDQ6674764.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7542..8591
                     /gene="truD"
                     /locus_tag="QBF46_002599"
     CDS             7542..8591
                     /gene="truD"
                     /locus_tag="QBF46_002599"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDQ6674765.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGELKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8572..9333
                     /gene="surE"
                     /locus_tag="QBF46_002600"
     CDS             8572..9333
                     /gene="surE"
                     /locus_tag="QBF46_002600"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDQ6674766.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9327..9953
                     /gene="pcm"
                     /locus_tag="QBF46_002601"
     CDS             9327..9953
                     /gene="pcm"
                     /locus_tag="QBF46_002601"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDQ6674767.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10093..11232
                     /gene="nlpD"
                     /locus_tag="QBF46_002602"
     CDS             10093..11232
                     /gene="nlpD"
                     /locus_tag="QBF46_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDQ6674768.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPAATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11295..12287
                     /gene="rpoS"
                     /locus_tag="QBF46_002603"
     CDS             11295..12287
                     /gene="rpoS"
                     /locus_tag="QBF46_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDQ6674769.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(12381..13745)
                     /locus_tag="QBF46_002604"
     CDS             complement(12381..13745)
                     /locus_tag="QBF46_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDQ6674770.1"
                     /translation="MSTITLLYIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(13834..14610)
                     /locus_tag="QBF46_002605"
     CDS             complement(13834..14610)
                     /locus_tag="QBF46_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDQ6674771.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYNYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGIKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(14615..15253)
                     /locus_tag="QBF46_002606"
     CDS             complement(14615..15253)
                     /locus_tag="QBF46_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDQ6674772.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(15250..16512)
                     /locus_tag="QBF46_002607"
     CDS             complement(15250..16512)
                     /locus_tag="QBF46_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HDQ6674773.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(16509..17417)
                     /locus_tag="QBF46_002608"
     CDS             complement(16509..17417)
                     /locus_tag="QBF46_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDQ6674774.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            17613..18380
                     /locus_tag="QBF46_002609"
     CDS             17613..18380
                     /locus_tag="QBF46_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6674775.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALVKQDLSLLVAKNE"
     gene            complement(18431..19087)
                     /gene="pphB"
                     /locus_tag="QBF46_002610"
     CDS             complement(18431..19087)
                     /gene="pphB"
                     /locus_tag="QBF46_002610"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDQ6674776.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYAIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPNSGRLSFYKIK
                     "
     gene            complement(19193..21754)
                     /gene="mutS"
                     /locus_tag="QBF46_002611"
     CDS             complement(19193..21754)
                     /gene="mutS"
                     /locus_tag="QBF46_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDQ6674777.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            21830..21964
                     /locus_tag="QBF46_002612"
     CDS             21830..21964
                     /locus_tag="QBF46_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674778.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            22041..22394
                     /locus_tag="QBF46_002613"
     CDS             22041..22394
                     /locus_tag="QBF46_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDQ6674779.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(22431..24509)
                     /gene="flhA"
                     /locus_tag="QBF46_002614"
     CDS             complement(22431..24509)
                     /gene="flhA"
                     /locus_tag="QBF46_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDQ6674780.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(24583..25593)
                     /gene="hypE"
                     /locus_tag="QBF46_002615"
     CDS             complement(24583..25593)
                     /gene="hypE"
                     /locus_tag="QBF46_002615"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDQ6674781.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAVACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNVAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(25590..26711)
                     /gene="hypD"
                     /locus_tag="QBF46_002616"
     CDS             complement(25590..26711)
                     /gene="hypD"
                     /locus_tag="QBF46_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDQ6674782.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(26711..26983)
                     /gene="hypC"
                     /locus_tag="QBF46_002617"
     CDS             complement(26711..26983)
                     /gene="hypC"
                     /locus_tag="QBF46_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDQ6674783.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(26974..27846)
                     /gene="hypB"
                     /locus_tag="QBF46_002618"
     CDS             complement(26974..27846)
                     /gene="hypB"
                     /locus_tag="QBF46_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDQ6674784.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(27850..>27965)
                     /gene="hypA"
                     /locus_tag="QBF46_002619"
     CDS             complement(27850..>27965)
                     /gene="hypA"
                     /locus_tag="QBF46_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="plays a role in hydrogenase nickel cofactor
                     insertion; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDQ6674785.1"
                     /translation="VTLLTQRVRRCPQCHGDMLQIVADDGLQIRRIEIDQE"
BASE COUNT         6623 a         7303 c         7492 g         6547 t
ORIGIN      
        1 cagttatgta tgcttagtgt gttccccgcg ccagcgggga taaaccgttt cagaaaagta
       61 cggctcaatt gatcgccccg tgttccccgc gccagcgggg ataaaccgcg ctggtggcgg
      121 aaaacagcca aaataacgca gtgttccccg cgccagcggg gataaaccgt ggattaatcc
      181 gcggctggcg atatctcgca gcgtgttccc cgcgccagcg gggataaacc gcgataaacg
      241 ctcaatacca gccggaataa tcggtgttcc ccgcgccagc ggggataaac cggcggaatc
      301 tgctgcgcac aagttacacg gcgcgtgttc cccgcgccag cggggataaa ccgccttgat
      361 cggattccag aaacgaacca ctaaagtgtt ccccgcgcca gcggggataa accgtcgttt
      421 agtgtggaat ttggcgacgc ggagcggtgt tccccgcgcc agcggggata aaccgtttag
      481 ctaatattgg gcggctaacg tcatcaagtg ttccccgcgc cagcggggat aaaccggtta
      541 aatattgttc accatcatgg atcaggaagt gttccccgcg ccagcgggga taaaccgcat
      601 gaacatggac gatgaaaaaa taagggaggg tgttccccgc gccagcgggg ataaaccgaa
      661 ttaaatgatt ttgatgcgcg ccgacttgaa gtgttccccg cgccagcggg gataaaccgc
      721 cacgcggctg cattcagtga tatccagttt ggtgttcccc gcgccagcgg ggataaaccg
      781 catctattta tctcgccgcg attggcgata acgtgttccc cgcgccagcg gggataaacc
      841 gccgaacggc tggcgaagca ggtggctggc gtagtgttcc ccgcgccagc ggggataaac
      901 cggtttaccg ccccgcagag gcgctggcag atccgtgttc cccgcgccag cggggataaa
      961 ccgggatgac ctgtcgctaa aactcgccgc gtacagtgtt ccccgcgcca gcggggataa
     1021 accgtccgtt tggtccacca aatgtttgat gcttcagtgt tccccgcgcc agcggggata
     1081 aaccgttcag ggtgtgattc cgcataccaa gcagtaagag ttccccgcgc cagcggggat
     1141 aaaccgggcg cactggatgc gatgatggat atcacttaga attccccgcc cctgcggtag
     1201 aacacccagc tcccattttc aaacccatca agacgccttc gccaactcct tcaccagagg
     1261 tagcattatc cgcataacgt cacggcagcg acgttctatt cttccaggaa gtgccttatc
     1321 aatatgctgt tgattatcca gtcttacgtc atgccagcta tttcccgcag ggaatgcggc
     1381 tgtttttgcg cgttgctgat aaccatcctt attcccaaga ttccagttag tcgcttccac
     1441 cgaaagtaca gcaatgcccg ctttgtcgaa tatttctgcg tcattacaac acccagtgcc
     1501 tttcggataa tttttattca aacccggatt ggtcgttgcg gctattccgt gactgcgtgc
     1561 aattgccagc gccctgtcgc gcgttaattt ccttactgct tcaggggttt ttacaccgct
     1621 gttgaaatac aatttatcgc caacaattaa gttatcgaga ttaatcacca gcagcgtatt
     1681 tttcttttcg gtgtcactca tccgcttgag taaattctca gcgcctaatt tcccttcctc
     1741 ttcgccgctg gtcgccacaa atcgaatacc atactcggta ggcgtatttt tcaggcgttc
     1801 tgccaattcc agcatgacac ctaaacctgc ggcgttatca tccattcctt gtaacgtcag
     1861 cccgccgaga ttggcatcgg cgtcagcatc gctcagcggg gcgtaagtat ccagatgcgc
     1921 cataatgatg atctgctgcg gcgctttgcc ttcatgagcg gcaatcaccg tacttcccgt
     1981 cacgttatgc cagctcttac gattatcgcg ggcggtataa atataccgac tattaaatgt
     2041 ccgaatatca ctgcgataac ccatttgctg aaactgttgg cgaatataat cggcagataa
     2101 catttctgca ggagttccgg tcatgcgtcc cggaaagaaa gtagcaatat gtcgtgcctg
     2161 agtattagca aaatcgccag gtttaggtga cgaggcgtgt acggggagaa taaagcatac
     2221 gccgagcgcc agggcagctg tacggtggcg caatgcggaa aacatagtga gtccttaaat
     2281 accatgcaaa tttttttacc gccatagtat gaaactgccg ctgcgctaaa acaatttcaa
     2341 atcttcctaa acgcccgaaa tccggtgcct taagcacttt ttgatattag ctttgccaaa
     2401 tcgttattcc gttaaggaac tactcattct aattggtaat ttcattcgtt ctcttacgct
     2461 ccctatagtc gaaacatctg atggcaagaa aatagcggta ttgcaaagga acggttatgg
     2521 atcaaatacg acttactcac ctgcggcaac tggaggcgga aagcatccac attattcgcg
     2581 aggtggcggc agaattctca aatccggtga tgctctactc tatcggtaaa gattccagcg
     2641 tcatgctgca tctggcgcgc aaggcgtttt atccaggtac gctgcctttc ccgttgctgc
     2701 atgtcgatac cggctggaaa ttccgcgaga tgtatgagtt ccgcgatcgt actgctaaag
     2761 cctacggctg cgaactgctg gtgcataaaa acccggaagg cgtggcgatg gggattaatc
     2821 cattcgtgca cggcagcgcg aaacataccg atattatgaa aactgaaggc ctgaaacagg
     2881 cgctgaacaa atacggtttt gatgccgcct tcggtggtgc gcgccgtgac gaagagaaat
     2941 cccgcgctaa agagcgaatt tactctttcc gtgaccgctt ccatcgctgg gatccgaaaa
     3001 atcagcgccc ggagctgtgg cacaactaca acgggcaaat taacaaaggc gaaagcatcc
     3061 gcgtcttccc gctctctaac tggaccgagc aggatatctg gcaatacatc tggctggaaa
     3121 atatcgacat tgttccgcta tatctcgctg cggaacgtcc ggttctggaa cgcgacggta
     3181 tgttgatgat gattgatgac aaccgtatcg acctgcaacc gggcgaagtg attaaaaaac
     3241 ggatggtgcg tttccgtacg ctgggctgct ggccgctgac cggtgcggtg gagtcaaatg
     3301 cacaaacact gccggaaatc atcgaagaga tgctggtttc caccaccagt gaacgtcagg
     3361 gccgcgtgat tgaccgcgac caggcggggt ctatggagct gaaaaaacgt caggggtatt
     3421 tttaagatga acaccgcact tgcacaacaa atcgccaatg aaggcggcgt cgaagcctgg
     3481 atgattgcgc aacaacataa aagcctgctg cgttttctga cctgtggtag cgtcgatgac
     3541 ggcaaaagta ctctgattgg tcgtctgctg cacgataccc gccaaatcta cgaagatcag
     3601 ctctcatcgc tgcataacga cagtaagcgt cacggcaccc agggcgaaaa gctggatctg
     3661 gctctgctgg tggacggcct gcaagctgag cgcgaacagg gcatcaccat tgacgtggcc
     3721 taccgctatt tctctaccga gaagcgtaaa tttattatcg ccgacacccc agggcacgag
     3781 cagtacaccc gcaatatggc gactggcgca tcgacatgtg aactggcgat cttactgatc
     3841 gatgcccgta aaggcgtgct cgatcaaacc cgtcgtcaca gttttatctc cacactgttg
     3901 gggatcaaac atctggtcgt ggcgatcaac aaaatggatc tggtggatta cagtgaagag
     3961 acgttcaccc gtattcgtga agattatttg acctttgccg ggcagctgcc gggtaatctg
     4021 gatatccgct ttgtgccgct ctctgcactg gaaggcgaca acgtggcatc gcaaagtgaa
     4081 agtatgccgt ggtacagcgg tccgacactg ctcgaagtgc tggaaaccgt ggagatccag
     4141 cgagtggtgg atgctcagcc aatgcgcttc ccggtgcagt acgttaatcg cccgaatctc
     4201 gattttcgtg gttacgccgg aacgctggca tccggtcgcg tggaagtcgg gcaacgtgtc
     4261 aaagtgctgc cctctggtgt ggaatcaaac gtcgcgcgga tcgtgacttt tgatggtgat
     4321 cgcgaagaag cctttgccgg agaagcgatc accctggtgc tgacggatga gatcgacatc
     4381 agccgtggcg atctgctgct ggcggcagac gaagcgttac cggcggtgca gagcgcgtcg
     4441 gtggatgtgg tatggatggc ggaacagccg ctttctccag ggcagagtta cgacatcaaa
     4501 attgccggta agaagacgcg cgcgcgtgtt gatggcattc gctatcaggt tgatattaat
     4561 aaccttaccc agcgtgaagt tgaaaacctg ccactgaatg ggatcggcct cgtggatctc
     4621 acttttgacg agccgctggt gttagatcgt tatcaacaaa atccggtgac gggtgggctg
     4681 atttttatcg atcgcctgag caatgtgacc gtgggtgccg gtatggtgca cgagccagtt
     4741 agccaggcaa ctgctgcgcc atctgaattc agtgcattcg aactggaatt gaatgctctg
     4801 gttcgtcgcc actttccgca ctggggcgcg cgcgatttgc tgggggataa ataatggcgc
     4861 tgcatgacga aaacgtcgtc tggcatagcc atccggtcac tgtgcaacaa cgcgagctac
     4921 accacggtca tcgtggtgta gtgctgtggt ttaccggcct ctccgggtcc ggtaaatcaa
     4981 cggtcgccgg ggcgctggag gaggcgttac ataaactcgg cgtcagtacg tatctgctgg
     5041 atggcgataa tgttcgccac ggattatgca gcgatctcgg ttttagcgat gccgatcgta
     5101 aagagaatat ccgtcgcgtc ggtgaagtgg cgaatttgat ggttgaagcc ggactggtag
     5161 tgctgaccgc atttatctcg ccacaccgcg ccgaacgcca gatggttcgc gaacgcgtag
     5221 gagaagggcg ctttatcgaa gtgtttgtcg atacgccgct ggcgatttgc gaagcccgcg
     5281 atccaaaagg cttatataag aaagcgcgtg ccggtgaact gcgcaacttt acgggaatag
     5341 attccgttta cgaagcgcct gaatcggcag aaattcatct caatggtgaa caattagtaa
     5401 caaatttggt acagcaatta ttagatctgt tgagacagaa cgatattatc agatcctgag
     5461 acaccaccgg gtttccatgc ccggttcgtc aaagtaacag gatcaaatat gcgtaatagc
     5521 cataacatta cactaacaaa taacgacagc cttactgagg atgaagaaac cacatggtca
     5581 ctgcctggtg ccgtggtcgg ttttatctcc tggttatttg cgctggcgat gccgatgttg
     5641 atttatggct ctaacacgct gttcttcttt atctacacct ggcctttctt tctggcgctg
     5701 atgcccgtcg cggtagtggt ggggattgcg ctgcattcat tgatggacgg aaagctacgc
     5761 tacagtattg ttttcaccct ggtgacagta ggcattatgt ttggcgcact gtttatgtgg
     5821 ctactgggct aatttgtact ttcccgtctc ctctgttcat aattcaaacc gtaactaata
     5881 atgagattat gttctgcacg ccctgggtat acgtaacaat ggacaaatgt ggtacatttg
     5941 cccgcgttgt cgcggtatcc ccaacagagg atgtagagtc gtcttcggat gcatgggatg
     6001 atgatgccgt ttttcagggg gcaggatggg taaactaacg ctgctgttgc tggctattct
     6061 ggtctggcta cagtattcgc tgtggttcgg taagaacggt atacatgact atacccgcgt
     6121 caatgatgat gtggcggcac agcaagctac aaacgcgaaa tttaaagcgc gaaacgatca
     6181 actttttgcc gaaattgacg atctcaatgg cggccaggag gcgctcgaag agcgtgcgcg
     6241 taatgaactc agcatgacca ggccgggcga aactttttat cgtctggtgc ctgacgcgtc
     6301 gaagcgcgca cagtctgcgg ggcaaaacaa tcgataaatc agcccgggaa ttaacatggc
     6361 aaccactcat ttggatgttt gcgccgtggt tccggcggcc ggatttggcc gtcgaatgca
     6421 aacggaatgt cctaagcaat atctctcaat cggtaatcaa accattcttg aacactcggt
     6481 gcatgcgctg ctggcgcatc cccgggtgaa acgtgtcgtc attgccataa gtcctggcga
     6541 tagccgtttt gcacaacttc ctctggcgaa tcatccgcaa atcaccgttg tagatggcgg
     6601 tgatgagcgt gccgattccg tgctggcagg tctgaaagcc gctgacgatg cgcagtgggt
     6661 attggtgcat gacgccgctc gtccttgtct gcatcaggat gacctcgcgc gattgttggc
     6721 gttgagcgaa accagccgca cgggggggat cctcgccgca ccagtgcgcg atactatgaa
     6781 acgtgccgaa ccgggcaaaa atgccattgc tcataccgtt gatcgcaacg gcttatggca
     6841 cgcgctgacg ccgcaatttt tccctcgtga gctgttacat gactgtctga cgcgcgctct
     6901 aaatgaaggc gcgactatta ctgacgaagc ctcggcgctg gaatattgcg gattccatcc
     6961 tcagttggtc gaaggccgcg cggataacat taaagtcacg cgcccggaag atttggcact
     7021 ggccgagttt tacctcaccc gaaccatcca tcaggagaat acataatgcg aattggacac
     7081 ggttttgacg tacacgcctt tggcggtgaa ggcccaatta tcattggtgg cgtacgcatt
     7141 ccttacgaaa aaggattgct ggcgcattct gatggcgacg tggcgctcca tgcgttgacc
     7201 gatgcattgc ttggcgcggc ggcgctgggg gatatcggca agctgttccc ggataccgat
     7261 ccggcattta aaggtgccga cagccgcgag ctgctacgcg aagcctggcg tcgtattcag
     7321 gcgaagggtt ataccctggg caacgtcgat gtcactatca tcgctcaggc accgaagatg
     7381 ttgccgcaca ttccacaaat gcgcgtgttt attgccgaag atctcggctg ccatatggat
     7441 gatgttaacg tgaaagccac tactacggaa aaactgggat ttaccggacg tggggaaggg
     7501 attgcctgtg aagcggtggc gctactcatt aaggcaacaa aatgattgag tttgataatc
     7561 tcacttacct ccacggtaaa ccgcaaggca ccgggctgct gaaagccaat ccggaagact
     7621 ttgtggtggt ggaagatttg ggctttgagc ctgatggtga aggtgagcat attctggtta
     7681 gaatcctcaa aaacggctgc aatacccgtt ttgtggcgga tgcactggcg aaattcctga
     7741 aaattcatgc ccgtgaagtc agcttcgctg ggcaaaaaga caaacatgct gttacggaac
     7801 agtggttatg cgctcgcgtg ccgggcaagg aaatgcccga tctgagcgcc tttcaactgg
     7861 aaggctgcca ggtgctggag tatgcgcggc acaagcgcaa gctgcgttta ggcgagctga
     7921 aaggtaacgc ctttaccctg gttctgcgcg aagtgagcaa tcgcgatgac gttgaacaac
     7981 gtctgatcga tatttgcgta aaaggtgtac cgaactactt cggtgcccaa cgttttggga
     8041 ttggcggtag caacttgcag ggggcgcagc gctgggcgca aaccaatact ccggtgcgcg
     8101 atcgcaataa acggagtttt tggttgtcgg cagcccgcag tgcgttgttt aatcagattg
     8161 ttgctgagcg cctcaaaaaa gcagacgtta atcaagttgt tgacggcgat gcgctacaat
     8221 tagccggacg tggtagctgg tttgtcgcaa ccaccgaaga actggcggaa ttacagcgtc
     8281 gcgttaacga taaagagctg atgataaccg ccgcattgcc gggcagtggc gaatggggaa
     8341 ctcagcgtga agcgctggca ttcgaacaag cagctgtcgc cgcagaaact gaattacaag
     8401 ctttactggt gcgcgaaaaa gttgaagccg cgcgcagagc gatgctgctg tatccgcaac
     8461 aattaagctg gaattggtgg gatgacgtca ccgtagagat ccgtttctgg cttccggcgg
     8521 gtagttttgc aaccagcgtt gtcagggaac ttatcaacac aacaggtgat tatgcgcata
     8581 ttgctgagta atgatgacgg ggtacatgca cccggtatac aaacgctggc gaaagccttg
     8641 cgtgagtttg ctgacgttca ggtggtcgcc cccgatcgta accgcagcgg cgcttcaaat
     8701 tctctgacac tggaatcctc cctgcgcacg tttacctttg aaaatggtga tattgctgtg
     8761 caaatgggaa ccccgaccga ttgcgtctat cttggcgtga atgctctgat gcgtccgcgc
     8821 ccggacattg ttgtgtccgg aattaacgcc gggccgaatc tgggggatga tgttatttat
     8881 tccggtacgg tagccgccgc gatggaaggc cgtcatttag gttttccggc gcttgccgtc
     8941 tcgcttgacg ggcataaaca ttacgacact gccgcggcgg taacctgttc aattttgcgc
     9001 gcactgtgta aagagccgct gcgcaccggg cgtattctta atattaacgt tccggattta
     9061 cccttggatc aaatcaaagg tattcgcgtg acgcgctgcg gtacacgaca tccggcagat
     9121 caggtgatcc cgcagcaaga tccgcgcggc aatacgctgt actggattgg cccgccgggc
     9181 ggtaaatgtg atgccggtcc ggggaccgat tttgctgcgg tagatgaggg ctatgtctcc
     9241 atcacgccgc tgcatgtgga tttaactgcg catagcgcgc aagatgtggt ttcagactgg
     9301 ttaaacagcg tgggagttgg cacgcaatgg taagcagacg cgtacaagca cttctggatc
     9361 aattacgtgc gcaaggtatt caggatgagc aggtgctgaa tgcacttgcc gccgtgccgc
     9421 gtgaaaaatt cgttgatgaa gcgtttgaac aaaaagcctg ggacaatatc gctttgccga
     9481 taggtcaggg gcagacaatt tcgcagccat atatggtggc gcgaatgacc gaattactcg
     9541 agctgacgcc gcagtcgcgg gtgctggaaa ttggcaccgg ttcgggatat caaacggcaa
     9601 tcctggcgca tcttgtccag catgtttgct cggttgaacg gattaaaggc ttgcagtggc
     9661 aggcacgtcg ccgcctgaaa aatcttgatt tacataatgt ttcaacccgt catggcgatg
     9721 gatggcaagg ttggcaggca cgtgcgccgt ttgacgctat cattgttacg gcggcaccgc
     9781 cggaaattcc aactgcgcta atgacgcagc tggacgaagg cgggattctc gtcttacccg
     9841 taggggagga gcaccagtat ttgaaacggg tgcgtcgtcg gggaggcgaa tttattatcg
     9901 ataccgtgga ggccgtgcgc tttgtccctt tagtgaaggg tgagctggct taaaacgtga
     9961 ggaaatacct ggatttttcc tggttatttt gccgcaggtc agcgtatcgt gaacatcttt
    10021 tccagtgttc agtagggtgc cttgcacggt aattatgtca ctggttatta accaattttt
    10081 cctgggggat aaatgagcgc gggaagccca aaattcaccg ttcgccgcat tgcggctttg
    10141 tcactggttt cgctatggct ggcaggctgt tctgacactt caaatccacc ggcaccggtc
    10201 agctccgtta atggcaatgc gcctgcaaat actaattctg gtatgttgat tacgccgccg
    10261 ccgaaaatgg ggacgacgtc tacagcgcag caaccgcaaa ttcagccggt acagcagcca
    10321 caaattcagg ctactcaaca accgcaaatc cagccagtgc agccagtagc tcagcagccg
    10381 gtacagatgg aaaacggacg catcgtctat aaccgtcagt atgggaacat tccgaaaggc
    10441 agttatagcg gcagtaccta taccgtgaaa aaaggcgaca cacttttcta tatcgcctgg
    10501 attactggca acgatttccg tgaccttgct cagcgcaaca atattcaggc accatacgcg
    10561 ctgaacgttg gtcagacctt gcaggtgggt aatgcttccg gtacgccaat cactggcgga
    10621 aatgccatta cccaggccga cgcagcagag caaggagttg tgatcaagcc tgcacaaaat
    10681 tccaccgttg ctgttgcgtc gcaaccgaca attacgtatt ctgaatcttc gggtgaacag
    10741 agtgctaaca aaatgttgcc gaacaacaag ccagctgcga ccacggtcac agcgcctgta
    10801 acggtaccaa cagcaagcac aaccgagcca actgtcagca gtacatcaac cagtacgcct
    10861 atctccacct ggcgctggcc gactgagggc aaagtgatcg aaacctttgg cgcttctgag
    10921 gggggcaaca aggggattga tatcgcaggc agcaaaggac aggcaattat cgcgaccgca
    10981 gatggccgcg ttgtttatgc tggtaacgcg ctgcgcggct acggtaatct gattatcatc
    11041 aaacataatg atgattacct gagtgcctac gcccataacg acacaatgct ggtccgggaa
    11101 caacaagaag tgaaggcggg gcaaaaaata gcaaccatgg gtagcaccgg aaccagttca
    11161 acacgcttgc attttgaaat tcgttacaag gggaaatccg taaacccgct gcgttatttg
    11221 ccgcagcgat aaatcggcgg aaccaggctt ttgcttgaat gttccgtcaa gggatcacgg
    11281 gtaggagcca ccttatgagt cagaatacgc tgaaagttca tgatttaaat gaagatgcgg
    11341 aatttgatga gaacggagtt gaggtttttg acgaaaaggc cttagtagaa gaggaaccca
    11401 gtgataacga tttggccgaa gaggaactgt tatcgcaggg agccacacag cgtgtgttgg
    11461 acgcgactca gctttacctt ggtgagattg gttattcacc actgttaacg gccgaagaag
    11521 aagtttattt tgcgcgtcgc gcactgcgtg gagatgtcgc ctctcgccgc cggatgatcg
    11581 agagtaactt gcgtctggtg gtaaaaattg cccgccgtta tggcaatcgt ggtctggcgt
    11641 tgctggacct tatcgaagag ggcaacctgg ggctgatccg cgcggtagag aagtttgacc
    11701 cggaacgtgg tttccgcttc tcaacatacg caacctggtg gattcgccag acgattgaac
    11761 gggcgattat gaaccaaacc cgtactattc gtttgccgat tcacatcgta aaggagctga
    11821 acgtttacct gcgaaccgca cgtgagttgt cccataagct ggaccatgaa ccaagtgcgg
    11881 aagagatcgc agagcaactg gataagccag ttgatgacgt cagccgtatg cttcgtctta
    11941 acgagcgcat tacctcggta gacaccccgc tgggtggtga ttccgaaaaa gcgttgctgg
    12001 acatcctggc cgatgaaaaa gagaacggtc cggaagatac cacgcaagat gacgatatga
    12061 agcagagcat cgtcaaatgg ctgttcgagc tgaacgccaa acagcgtgaa gtgctggcac
    12121 gtcgattcgg tttgctgggg tacgaagcgg caacactgga agatgtaggt cgtgaaattg
    12181 gcctcacccg tgaacgtgtt cgccagattc aggttgaagg cctgcgccgt ttgcgtgaaa
    12241 tcctgcaaac gcaggggctg aatatcgaag cgctgttccg cgagtaagta agcatctgtc
    12301 agaaagacca gtctcaagcg aggctggcct tttctgtgca caataaaagg tccgatgccc
    12361 atcggacctt tttattaagg tcaaattacc gcccatacgc accaggtaat taagaatccg
    12421 gtaaaaccga gaatggtcgt taacactgtc caggttttca gaccgtctgc taccgacaac
    12481 cccagatatt tggtcacaat ccagaaccct gagtcattaa tatgtgacgc gccaagccca
    12541 ccaaagcagg ctgccagcgt caccaatacg cactgaatcg gattcaatcc catcaccgct
    12601 tctgagagta acccgccggt tgtcaggatt gctacggttg ctgacccctg cgatgcacgc
    12661 agcgccagtg aaataataaa tgcggctggt aacagaggca ggtcaatcat ttgtagcatg
    12721 ttggcaaggg ctttgccgac gcccgattcc accagcactt tgccaaatac ccctccagca
    12781 ccagtaacca aaatcactac cgccgcagta ggaagcgctg agcccataat gtcgctggtg
    12841 tgttgtaagc tccagccgcg acgtaaagcc aataaccaga atgccagcac cagcgcaatc
    12901 attagagcta ccattggtga gccgatcagc tgtagcgtac caagcagggg atgcgaaggc
    12961 ggcatcagtg ttgcggaaac cgtacccgcc atgataatcg cgataggaat aacaattagc
    13021 gaggtgacca gcgcgacgcc cggtggattt attttatcgc ttaattttgt cgcgccttcc
    13081 tcactggccg gagccagttg catctgttcc agtacttcca ccgacatcgc atattggcgc
    13141 ttattgatta ttttcgctgc aaagtagcca acaatcccta cgggaataga aatcgcaata
    13201 ccgatgatgg ttagccagcc gatgtctgcg tggagtaacc ctgctgcggc gacagggcct
    13261 ggatgcggcg gtaccgccac atgtacagtt agcatgatcc cagcgacagg caggccaaat
    13321 ttgagtggcg atattttggc aaccttggca aaaccgtaaa tgattggcgc aagaataata
    13381 aagccgacat caaagaagac gggaataccg aggaagaacg ctgccagagt cagcgcagcg
    13441 atagttcgtt tgtcacctaa cttgcgactg aaataattag ccagtgactc tgcaccacca
    13501 gagtgttcga tcatacgccc cagcatagcg cccagaccaa taataatagt gacggaacca
    13561 agcacaccgc ccatcccggc gatcatcact ttacccactt cgcccgccgg tatacccgcc
    13621 gcaagtgcga ctaacaggct gacgaggagc agagcaacga atggttgtac ctttgccttg
    13681 atgaccagca gcaacagcat gattacgcca gttaacgcaa tgtataacaa tgtaattgtg
    13741 gacatgggaa accctgtctg aaagttatag ttaacctacc ccatccgtag atggggggat
    13801 gtatgggtac gttgtaatta gggatttaac gaattagcgc caggcgtcaa accagccaag
    13861 tccttcttcg gtgaggccac gaggtttata ttcacaaccg atccagccct gatatcccac
    13921 ctcatcgaac aggcggaaca gccacggata gttgatttct ccatcgtccg gttcatgtcg
    13981 atcaggtagt ccggcaattt gtacgtgcgc atatttcccg gcgtagtcgc ggattaaatg
    14041 cgtcaggttg ccatctactt tttgcgcatg aaaagtatct agttgaataa acacgttatc
    14101 tcgcgcaact tcttcaacaa tagccagtgc ctgatactgg ctggagaaga gataatgagg
    14161 cttaatgccg gggctgagtg cttcaactaa tattcgcttg ccgtgtggcg caaagcggtc
    14221 ggcagcgtag cggagattat cgataaatac tgcccggtac cgttcagcat cttcgccagc
    14281 gggcacgacg cctgccatta catggacttg ttcacaattg agcgccaatg catattccag
    14341 tgccaggtcg atgtctgcgt gtgcttcgtg ctcacgtccg ggaagggcgg ataatcccca
    14401 ttcccccgca ttaatatctc cgggagcggt attgaacagc gccagtgtca gatggttttg
    14461 ctccagttgc ttttggattt gcagggtgga gtagttatag ggaaacagaa attccacagc
    14521 atcgaacccg gcttttcgcg ctgcggcgaa gcgttcaata aaaggcactt cggtgaacat
    14581 catggataaa ttagctgcaa aacgaggcat tgcattaact ccttaattcc gcaatttcac
    14641 ctgcggtcag ataacggatc gggcggtcac cgagaataaa aatcagcttt gccgtttcct
    14701 ccagctcttc catattgttg gcggcttctt gcaggctttc accgcaaacc actgggccat
    14761 gatttgccag taaaaaagcc tgattgtctg ctgccagttc cgccagatcc tgtgcgatgc
    14821 gtttatcgcc cggtcggtaa taaggcacca gcgggacatt tcccatccgc atcaccacgt
    14881 atggtgtgaa cggacgaata acgttgctgc tgtccagccc ttgcaggcag gaaagcgccg
    14941 tcgaccatgt gctgtgcaaa tgcaccaccg ctttacagcg cggattgttg cgatacagcg
    15001 ccagatgaaa gagcacctct ttcgagggtt tgtcaccact taaccattcg ccatccgcgg
    15061 cgactttgga aagccgctgc ggatcgagat tgcccaggca tgaacctgtc ggtgtcgcca
    15121 gtaaattccc gtcaggtaaa agcagcgaca gattgccagc cgaaccggtt gcatagccgc
    15181 gctgaaagaa tgaactggca atccgcgtca tctcctctcg caaagactgc tctacttttg
    15241 cgaaatcgct catgataaaa actctctttg ggctcgtgaa aaaaaggctt catcaccgaa
    15301 gttgccagat ttaagggcga gtgagacagg cttatccagt gcgtttaccc acggcacgcc
    15361 gggggaaatg gttgggccaa tatgaaaccc ttttatgccc aggctctgtg tgactacgcc
    15421 ggaggtctca ccgcctgcga caataaagcg tgtcacgcct tccgctgcta accgcgccgc
    15481 tagttgagaa aacagagttt ctactgcctg actggctttt tgtgcaccgt attgctgttg
    15541 aattgctgcc aatgcgtcag tgctggcggt ggcaaaaacc agtggagcaa gtacactttc
    15601 ctggcccaga acccactctg ccagttcgtg tgcataagcg gccagagttt cggttgagag
    15661 gcagcgtgcc acatcaactt cacgggctgg tgcaatttga cggtaatgtg ccacctggcg
    15721 gttggtcatt tgagagcatg aaccggagag cactacgccg cgcccagcga gcggacgccc
    15781 tgcttcgcga gcctggttac cgttttcttg cgcccactgc cgggccaggc caatcgccag
    15841 accagaaccg cccgttacca gtggggcatc gcgcaaggct tctccctgaa tttccagatg
    15901 gtgttcggtc agcgcatcaa gcaccgcgta gcggtagccc tcttgctgta agcgagccag
    15961 ctcttgacga acggcatcca caccttgttc gaaaacatgt gccgaaacga cgccgcagcg
    16021 ccctgtggat tgcgcttcaa ccagacgggg aagatagctg tcggtcatgg gatttaccgg
    16081 gtgatggcgc atcccggatt cggccagcag ttgattcatt acgaacaaat acccctgata
    16141 aaccgtacgt ccgttgaccg gcagggccgg agagaagacc gtaaacggcg tgtcgagagc
    16201 atccattaat gcatcggtaa ccgggccgat attaccttta gccgtactgt cgaaagtaga
    16261 gcagtatttg aaatagatct gtttgcaacc ttgctgttgc aaccagctca gagccgccag
    16321 cgattgctgt gtggcttcaa ccaccggaca ggaacgcgtt ttcaggctga tcaccagtgc
    16381 gtcgattgct tccggcattt tacctgttgg aacaccgtta atttgtaccg ttggtagacc
    16441 gttttccacc agaaaactgg cgatatccgt cgcgccggta aaatcatcgg cgataacgcc
    16501 aatcttgatc atgatttcgc tcccggtaga gtgatgccag agaaaatctt gataactgcg
    16561 ctatcgtctt ctttcccgta acccgcgtta ctggcgctgg tgaacatatt caatgctgtt
    16621 gaggccaatg gcagcgggaa gtgcagggct ttggctgtat cggcaaccag accaagatcc
    16681 ttaacaaaaa tatcgacggc tgaatgcggg gtgtaatcgc catccaccac atgacgcatc
    16741 cggttttcga acatccagga atttccggcg gcattggtca cgacgtcata catcacatcc
    16801 agcgggatcc ccgcacgggc tgcaagtgcc atcgcttcgg ctccggcagc aatatgtacg
    16861 cccgctaaca actggtgaat aatttttacg gtcgaaccta gtcccggttc tgcacctatg
    16921 cgataaactt ttccggcaac ggcttccagc acgggtgcca gtcgttcaaa ggcaatatcg
    16981 ctaccggagg ccatgacagt catttcaccg ttagcggctt ttactgcacc accagaaact
    17041 ggcgcatcca gcatttccag atcgaatcca gccagagcgg tagcaatttc ttgcgcatca
    17101 gcactagcga tagtggaaga aaccatcact gccgtaccgg gtttcagatg ttgtgcaacg
    17161 cctgtttcac caaacagcac ctgtttaacc tgggccgcat tgaccaccag caccagcaga
    17221 gcgtccagtt tttcggcaaa cgtcgcggcg ttatcagaaa ccccgcaagc acctgcctct
    17281 ttcaacgtag cgcaggcatt gctgttcagg tctgcgcccc aggtagaaag acctgcgcgg
    17341 acacatgaca gtgctgctcc cattcccatt gaccctaagc caacgatacc gacatgaaac
    17401 tcagatcccg ttttcatctg ctctccttgt taatttaagt gatattttgt ttgatattgt
    17461 gaatataagc gctggaagat aacgatatgg tgagctgatt cacataaatt aacattgtgt
    17521 gttattttat gtgaactaag cgttagttgc gccgcgcacg tttcgcaggc aaatagcgta
    17581 gaatgtcagc aggacaaagg aaggagcaaa agttgatacc cgtagagcgt cgccaaatca
    17641 tccttgagat ggtagctgaa aaaggcattg tcagtattgc tgaactaacg gacagaatga
    17701 atgtgtcaca tatgaccatt cgtcgggatt tacaaaaact ggagcagcag ggagccgttg
    17761 tgctggtgtc cggaggcgtc cagtctccgg gacgcgtggc gcatgaacct tctcatcagg
    17821 taaaaactgc gctggcaatg acgcaaaaag cggctattgg caagctggcg gcaagtcttg
    17881 ttcagccggg aagttgtatc tatctggatg cgggaacgac cacgttagcg atagcacagc
    17941 atctgattca catggagtca ctgactgtgg tcacaaacga tttcgttatt gcggactact
    18001 tgctcgacaa cagtaattgc acaattattc acactggcgg tgcagtgtgt cgggaaaacc
    18061 gttcctgtgt cggggaagcc gctgcgacca tgctgcgcag cctgatgatt gatcaggctt
    18121 ttatttctgc atcgtcatgg agtgtgcggg ggatttctac gccagcggaa gataaagtca
    18181 cggtgaaacg ggcgattgcc agtgccagcc gccagcgagt tttggtctgt gatgcgacga
    18241 aatatggtca ggtggcgaca tggctggcgt taccattaag cgagtttgat cagattatta
    18301 cagacgacgg tttgccggag agtgccagtc gcgcgctggt gaagcaggat ctctctttgc
    18361 tggtagcgaa aaatgaataa tggcctgcaa taacaccggg ttactcatgc ttcacagaag
    18421 aagcatgaga ctactttatt ttataaaatg acagccgccc gctgttcggc gatccggtat
    18481 caatataaat ctggttagcg aacgtctgaa tgttatcaaa catcatatgt ccaaatataa
    18541 aataatcagc gccgtttatt tgttgtaact cgccattaag cgatttctgc acacgatcaa
    18601 caggccagag taattcgctc tccgctattt ctttaccaaa gagatattca ctccccggat
    18661 aatctgcatg tgcgatggca tattttatgt tgtcgttagt gatttcaata atatgtggaa
    18721 ggtgatggaa tttcagcaac agatctattg cctcttgttg ctctgaatca tttaaatcga
    18781 aaaaccagtc accaccgctg gcaagccaca tattgccatc gccagtttcg aatgcctcaa
    18841 gcgccatcgc ttcgtggttg cctttaaccg acgtaaacca gggttggttt agcaggcgca
    18901 gcacgttaag actcttcggc ccacgatcaa tattatcgcc tgtggaaata agtaagtcgg
    18961 tttcagggta aaaagagagt tgatgtaaac gggattgtaa taactgatat tcaccatgaa
    19021 tatcaccaac gacccatata tggcgatagt gatgggcatt gattttttga tagcgtgtag
    19081 atggcatggt tttaccctgt aaaataagct ttcctattat acagggtatt tttatttgat
    19141 tcgtcagttg tcgttaatat tcccgatagc aaaagactat cgggaattgt tattacacca
    19201 gactcttcaa gcgataaatc cattccagcg cctgacgcgg agtcagtgaa tccgggtcga
    19261 gattttccag agcttcgacc gctggcgagg tttcttccgg tactgacagc aaagacattt
    19321 gcgtaccatc cacttgtgta gcggcggcgt tcggcgaaat actttccagc tcacgcagtt
    19381 tttgccgtgc gcgcttaatc acctcttttg gcacgcccgc cagagctgca accgccaggc
    19441 cgtagctttt gctcgccgcg ccatcctgca cgctatgcat aaaggcaatg gtgtcgccgt
    19501 gctccagcgc atcgagatgc acgttggcga cgccttccat tttctccggt aactgggtca
    19561 gctcgaaata gtgggtagca aataacgtca atgccttaat cttattcgcc agattttccg
    19621 cgcacgccca cgccagcgac agaccatcgt aggtggacgt tccacgcccg atctcatcca
    19681 ttaacaccag actgtattcg gtggcgttat gtaaaatatt ggcggtttca gtcatctcca
    19741 ccataaaggt tgagcgcccg gaagccaggt catctgccgc gcctacgcgg gtaaagatgc
    19801 gatcgatagg tccaatctcg actttttgtg ccggtacata gctgccgatg taggccatca
    19861 gcgcaatcag tgcggtctgg cgcatatagg tacttttacc gcccatgttc gggccggtga
    19921 tgatcaacat gcggcgctgc ggcgacagat tcagcgggtt ggcgataaat ggctcattca
    19981 gtacttgttc aactaccgga tggcgacctt cggtaatgcg aatgcccggt ttatcaatga
    20041 aggtcgggca ggtgtagttc agggtatagg cccgttccgc caggttcacc agcacgtcga
    20101 gttccgccag cgcgctcgcg ctctgttgca acgcttccag atgcggcaac agcaggtcga
    20161 acaactcttc ataaagctgt ttttccagtg ccagtgcttt gccttttgag gtgagaactt
    20221 tatcttcgta ctcttttagc tctggaatga tgtagcgctc ggcgtttttc agcgtctggc
    20281 gacgcatgta gttgatgggt gccagatggc tttgcccacg gctgatttga atgtagtagc
    20341 cgtgcaccgc attaaagcca actttcagcg tgtccaggcc ggtacgttca cgctcgcgga
    20401 cttccagacg ctccagataa tcggtcgcgc cgtcagccag cgcgcgccac tcatccagct
    20461 cttcgttata gcccgatgcg ataacaccac cgtcgcgtac cagcaccggc ggtgtgtcga
    20521 tgattgctcg ctccagcaga tcgcgcagct cggcaaactc gcccatcttc tcacgtagcg
    20581 cctgtaccgg tgcactatcg acagtttcta actgcgcacg cagctccggc agttgctgga
    20641 aagcgtggcg catacgggcc agatcgcgtg ggcgagcagt tcgtaaagcc agacgtgcca
    20701 gaatacgttc caggtcgccg acctgacgca gtaccggctg caactcggcg gtgaaatcct
    20761 gcaatgcgcc aatagtttgc tggcgctcaa gcaacacgcg ggtatcgcgc actggcatat
    20821 gcagccagcg tttcagcata cgactaccca tcggcgtgac ggtgcagtcg agcacagaag
    20881 ccagcgtatt ttccgcaccg cccgccaggt tctgagtaat ttccaggtta cgacgtgtcg
    20941 cggcatccat aatgatgctg tcctgctcac gttccatggt gatagaacga atatgcggca
    21001 gggtcgtgcg ttgggtatct ttcgcatact gcaacagaca accggcagca caaagtccgc
    21061 gcggcgcgtt ctcgacgcca aaaccgacca gatcgcgggt gccaaattgc agattcaact
    21121 gctggcgcgc ggtgtcgatt tcaaactccc acagcgggcg acggcgcagg ccgcgacggc
    21181 cttcaatcag cgacatctcg gcgaaatctt ctgcatacag cagttccgcc ggattagtgc
    21241 gttgcagctc tgccgccatc gtttcgcggt cggccggttc gctcaggcga aaacgcccgg
    21301 agctgatatc cagcgtcgcg tagccgaaac ctttgctgtc ctgccagata gccgccagca
    21361 ggttgtcctg acgctcctgt aacagggctt catcgctgat ggtgcctggc gtaacgatac
    21421 gcacaacttt gcgctcaacc ggacctttgc tggtcgccgg atcgccaatt tgttcgcaga
    21481 tggcaacgga ctctccctga ttcaccagtt tggcgagata gttttccacc gcatggtagg
    21541 gaatccccgc catcgggatc ggctctcccg ccgaagcacc gcgtttggtc agtgaaatat
    21601 ccagcagttg cgacgcgcgt tttgcgtcgt cataaaacag ttcataaaaa tcacccatcc
    21661 ggtaaaacag caggatctcg ggatgctggg ctttcagcct gagatactgc tgcatcatgg
    21721 gcgtatgggc gtcgaaattt tctattgcac tcatggggtt atgtccggtt ccctgatatt
    21781 aaatggggtg tgatggtttt tattttcgtt gtaatcacat aaggcgcaca tgataccgga
    21841 gttaatcatt tcaggggaag aagcgaacgg cgagttcttg atgctggttc cgggaaagat
    21901 gctgttttgc agcttttgcg tgatgcttat gaagcaagta cgcaaaattg cgtcggttga
    21961 ataaacctcc tccttcagag ccatagtccc ctttcaaatt aagttgtaat ataaatacac
    22021 cttaagagta tggaggcagc atgtccggca agcgtatctc tcgtgaaaaa ctgacgatta
    22081 aaaaaatgat cgatctttat caagcgaaat gcccgcaggc gtcagcggag ccggagcatt
    22141 acgaggcgtt gtttgtttac gcgcaaaagc ggctggataa atgtgtgttc ggcgaagaga
    22201 aaccagcctg taagcagtgt ccggtgcact gttaccagcc agcaaagcgt gaagagatga
    22261 aacagattat gcgttgggcc gggccgcgaa tgctgtggcg tcatccgatc ttaaccgtgc
    22321 gtcatctcat cgatgacaag cgtccggtgc cagaactgcc ggaaaaatat cgtccgaaaa
    22381 aaactcgtga atagtatgcc ggacaggcag atctgtccgg caatttgcag ttaaatcaat
    22441 gccgatttat caattccaag ccgcttcatc cgtgacagca aggtcgtgcg tttcagcccc
    22501 agacgttgcg cagcgccttt aggcccggca accacgccgt tggtttcttt cagcacgcga
    22561 acaatcaact gatattcatc ttcgccctcc tgggcgacaa ccgttgcggc aggcggcgtt
    22621 tcaggttccg gtaaaacaat atctggcaat gacagctgca gcacgttacc gcgtgttagc
    22681 aataccgcgc gctcaatgac gttttccagt tcgcgtacgt tacccggcca ctccatgttg
    22741 ctcaaggtgc gcagcgtctc ggcaggaatg ctgtcgatat tgcgccccag acgacgggca
    22801 attttgaagg taaaggcttt cgccagcagc ggaatatctt ccggacgctc gcgtagtggc
    22861 ggcaggtgaa tcgggaatac gttcaggcgg taatagagat cgctacggaa ctcacggtcg
    22921 gcgaccattt ttttcagatc gcggttagtc gcggcgatta gacgcacgtc cgtctgaatg
    22981 attttgttgc tgccgagacg ttcaaactcc tgttcctgca atacacgcag caacttcggc
    23041 tgtaactcca gtggcatatc gcccacttcg tcgaggaaca gggagctttt atccgccagt
    23101 tcaaaacgac caatgcgctg ggcgctggca ccggtaaaag ccccacgctc atgaccaaac
    23161 agatcgcttt ccagcaatcc ggcaggcatc gccgcgcagt tcattttgac catgcggcga
    23221 ttattacgcc cactgagatt atggatcgca cgggcaatca gctctttacc cgtgccagtt
    23281 tcaccgagga tcagaacggt actgtcactt tgcgccacca tttcaacttg tttaagcacg
    23341 ctgtacatgg cttcgctgcg gccaataatc tcgccaaatt cactatcaac attgttgagc
    23401 tgctcggtca gggcgaggtt ttcatcaacc agccgttctt tcagacgatg gatttcctga
    23461 taggcgaggg cgttatcgac agcgattgcc acacgttcgg caatctggcg cagtaaattc
    23521 agattggtag tggtaaacac tttctcttcg cattgcgcca gtttcagcac gcccagcatg
    23581 gtgtcgccag acatcagcgg taacaggcac aaggtttgaa tctggttgcc ccaggtgtcg
    23641 aacaacatgc gttcataggg ggctaaatcg tcccgctcgt ggagattgat cagcagcatc
    23701 tctttacttt tgaacacgcg ttcggtgagg gttccggctt catcgacttc gctctgttcg
    23761 tgggcgggat gttgtttatc aagatagtga gtggagtaga tgttgagttt gtttttacgg
    23821 tggctgcgta agacaatgct gatatcgtca atgtcgaaat agtaatggat ttctttggcg
    23881 acttcgctga ccagttcgtc catatccagg cgggaaagca ccgcgttggt aatggcaacc
    23941 aggatgcgga agttatcgcg ctcccggcat aacaactcat agtcgacatt gttgacgacg
    24001 cggctctgga tttgttcggt gacgacagaa acgatctgcg taaatgtttg cagacgattg
    24061 aactcttttt cgctccaggg gcgatcgtca taacgaataa attcacagcc accaaaaata
    24121 tgcccttccg ccgccagtgg catcaggcaa tagtgaccaa atttggggta tagcccacct
    24181 gcggccagct gcggccaggt ttcacaaaat tcttcgtaac tgcaatgcag cgtatcaggg
    24241 cgcgacaaaa tgctgcgtac cggaccgtgt gccagaacag tttcgtcttc atatttaatg
    24301 ggggtgtctt tttcacgcga tgcgtaataa gacgcacgtt gagtctgcgc ttgccacaac
    24361 acaatagccg cgttgtcggc gagcgcagaa cgctttacca gttgcgaaag agcctcacac
    24421 agcgaggcca gatcgggctg ctgcaatagt gtccgagtga tgtcgaacaa cccttgctgt
    24481 ccgagatcac tcatcggtgt atatgacatt ttgttcttcc aggaaagcac cgcaaagcgg
    24541 tgcaaaaggt cattgactaa ctataggaga tttagaattt tattagcata tacgcggaag
    24601 cggttcggcg tgtggtaaat cgagggttcg tttcacgcca tacagaccgg caagacgaac
    24661 acctttacgt tccaccactt caccaatcag cgccgcgtct tttcccagtg gatgggaatg
    24721 taacgctgcc agcacttgct ctgccacgtt gcgttcaacg gcgatcacca gtttgccttc
    24781 gttggcaaag ttcagggcgt ccagtcccag cagttcgcaa acgccgcgca cggcaggttt
    24841 aaccggcagc gccgcttctg aaatttcaat accacaaccg caggctaccg cgaactcatg
    24901 aaccaccgcg tttacaccac cacgggtggc atcacgcagc gctttcacgc cgggaatgtc
    24961 acgcagcgtc tgaataagcg gcgtcagcac cgcgcagtcg ctgaccagtt cgccatccag
    25021 ccccagctgc tcacgcaggt taaggatagt cgccccgtgg tcgccgagtg taccgctaac
    25081 cagcaataca tcgcctgcgg ttagcgtctg tgcgccccag tgaatattcg ccggaattgc
    25141 gcccatgcca gcggtgttga taaacagttt atctgccgcg ccgcgctgca ccactttagt
    25201 atcgccagta acgatggcaa tgcctgccgt gcgggcggtt tctgccatgc tggtcactac
    25261 ggctttcagt gtctccatcg gcaatccttc ttcgaggata aagccacagg agagatagcg
    25321 cggaatagcg ccactgaccg caacgtcatt cgcggtgccg caaatcgcca gcttgccgat
    25381 attaccgcca ggaaagaaca gcgggtcaat gacgtaactg tcggtggaga acgccagacg
    25441 gtcgccttcc gctaccagct gcgccagatc aagacgtgcc tgatcttcct gctctgccag
    25501 ccacgggttg gcaaaggctt ccataaacag gctgttgatt aattgctgca tcgcctggcc
    25561 gccgctgccg tgggcgagtt ggatattatt cacgcttcac tctcctgctg acgatactga
    25621 taccacgcgg cgcacgctcc ttcggaggag accatcagtg caccaaatgc ggtttgagga
    25681 ttacaggtgt taccaaacag cgggcattga tgcggcttac atttgcccgt taatacctca
    25741 ccacaacgcg cgcgcgggtc atcgcagacc tgctgcggtg ccgggcggaa atgtgcttcg
    25801 gcatcgaatc gttgataatc cggcgtcagg tgcacgccag aagattcaat cacgcctaag
    25861 ccgcgccatt cgctgtcgcc gttgacacag aacacatcag caattgcctg ttgcgccagc
    25921 aggttaccgg catccggcac cacccgacga tactgattct ctaccttgct gtgggccgct
    25981 attttctgct ccaccagcat caccacgcct tgcagtagat caaggggttc gaaaccagcg
    26041 accaccagcg gacgatgaaa atcgctggcg ataaaattat aggcatcagt gccgataacc
    26101 atactaacgt ggcccggcgc gaggaacgcg tcgataccgt tatccggctg ttccagcaaa
    26161 ctgcgcagcg tcgggataag cgtaatgtgc tggcagaaga agtaaaaatt ctgcacatca
    26221 cgcgctttcg cctgttgcag agtgatagcg gtggtcggca tagtggtttc aaaacctaag
    26281 ccgaagaaca ccactttgcg ggttggattc tcctgcgcca gtttcaacgc atccatcggc
    26341 gagtaaacga tgcgcacatc ggcaccgcgt gcttttgcct gcaacagcga tccctgtttc
    26401 cccggcacgc gcatggcgtc gccaaaggta cagaagatga cttccggatg gctggcaatc
    26461 tccacgcagg tgtcgattct acccatcggc agtacgcaca ccgggcaccc cggaccgtgg
    26521 ataaactcaa cgttttccgg cagtaactgg tcgaggccga atttaaagat agcgtgggta
    26581 tgaccgccac acacttccat aatccgcaga gggcgttcgg cggtgtaaga gagatgtgaa
    26641 gcacgttcgc gcagatgctc aattaactgc atcacctgtt ccggcgcgcg atattcatca
    26701 acaaaacgca ttatttttcc tcgccataca acagcgcgcc gacatccggc tcaacgtcaa
    26761 acatgttttg taaggcgtcg agagtgtcgc gtgcttcggc ttcattaatt acgctcatgg
    26821 caaagccaac gtgtaccagt acccactggc ccacgcgcgg ctgaccgttt tcatcgcagc
    26881 tgccgactaa cgttaaatcg acatcgcgct gaatgccgca gacgtcgact ttcgcctggt
    26941 tgccgtcaat ggtgcggatc tggccgggaa cgcctatgca catcgctgtg tctccagcca
    27001 gttcagccac tggtccatcc cttcgccgct ggtggcggaa ataaggatga tttcaatttc
    27061 tggattgact tcgcgggcgc aggcgatgca cttttcaacg tcaaagttga gatacggcaa
    27121 caggtcaact ttgttgagca gcatcagcga ggcggcggca aacatatgcg gatatttcag
    27181 cggtttgtct tcaccttcgg taacggaaag caccgccact ttgtgttttt caccgagatc
    27241 gaagctggcc gggcatacga ggttgccaac gttttcgata aacagaatac cgttatcgtc
    27301 cagtggcaga cgcggtgcgg cgtcggcaat catctgtgcg tcaagatggc agcctttacc
    27361 ggtgttcacc tgaatcgctg gtgtgccggt agcgcgaatg cgtgcggcat cgttcacggt
    27421 ttgctggtcg ccttcaataa ctgcgcacgg aacgctgtct ttcaggcgca ttagggtttc
    27481 cgtcagcagg gtggttttac cggaaccagg gctggaaacc aggttgagca ccagttgctt
    27541 gcgggcagca aagcgcgcgc ggttgcgttc agccagacgg ttatttttgt ccagcacgtc
    27601 aatttcgact tccagcatcc gacgctggct catgcccggt gcgtgagtgc ccgcttcgcc
    27661 atgaccgtaa tgcaggtcgc cttcttcagt ctggctgggg gtaaattcag gcgctttaat
    27721 gccggtgatt ttcatcttcg ggcgtgccgc cggggcaaat ggcgcgctac gaaacgcgga
    27781 atgagggtta tgttcatcac cctcgatata caggttgcct tcaccgcaac cgcatgttgt
    27841 acacatcgct cactcctggt ctatttctat ccgccgaatc tgtaaaccgt cgtctgccac
    27901 aatctgcagc atgtcaccat gacactgtgg acagcggcgg acgcgctggg tcagtagcgt
    27961 cacat
//
LOCUS       DAOFQD010000061        27592 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.061, whole genome shotgun sequence.
ACCESSION   DAOFQD010000061 DAOFQD010000000
VERSION     DAOFQD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.061"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(280..543)
                     /gene="cedA"
                     /locus_tag="QBF46_002620"
     CDS             complement(280..543)
                     /gene="cedA"
                     /locus_tag="QBF46_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDQ6674786.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            726..2987
                     /gene="katE"
                     /locus_tag="QBF46_002621"
     CDS             726..2987
                     /gene="katE"
                     /locus_tag="QBF46_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDQ6674787.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3245..4003)
                     /gene="chbG"
                     /locus_tag="QBF46_002622"
     CDS             complement(3245..4003)
                     /gene="chbG"
                     /locus_tag="QBF46_002622"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDQ6674788.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(4016..5368)
                     /gene="celF"
                     /locus_tag="QBF46_002623"
                     /gene_synonym="chbF"
     CDS             complement(4016..5368)
                     /gene="celF"
                     /locus_tag="QBF46_002623"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDQ6674789.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHMDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5472..6314)
                     /gene="chbR"
                     /locus_tag="QBF46_002624"
     CDS             complement(5472..6314)
                     /gene="chbR"
                     /locus_tag="QBF46_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDQ6674790.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6322..6672)
                     /gene="chbA"
                     /locus_tag="QBF46_002625"
     CDS             complement(6322..6672)
                     /gene="chbA"
                     /locus_tag="QBF46_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDQ6674791.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRLALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6723..8081)
                     /gene="chbC"
                     /locus_tag="QBF46_002626"
     CDS             complement(6723..8081)
                     /gene="chbC"
                     /locus_tag="QBF46_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDQ6674792.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8166..8486)
                     /gene="chbB"
                     /locus_tag="QBF46_002627"
     CDS             complement(8166..8486)
                     /gene="chbB"
                     /locus_tag="QBF46_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDQ6674793.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVATIKK
                     AAAN"
     gene            complement(8785..9123)
                     /gene="osmE"
                     /locus_tag="QBF46_002628"
     CDS             complement(8785..9123)
                     /gene="osmE"
                     /locus_tag="QBF46_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDQ6674794.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9325..10152
                     /gene="nadE"
                     /locus_tag="QBF46_002629"
     CDS             9325..10152
                     /gene="nadE"
                     /locus_tag="QBF46_002629"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDQ6674795.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQSLREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10382..11269
                     /gene="cho"
                     /locus_tag="QBF46_002630"
                     /pseudo
     CDS             10382..11269
                     /gene="cho"
                     /locus_tag="QBF46_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
     gene            complement(11229..11804)
                     /gene="ves"
                     /locus_tag="QBF46_002631"
                     /pseudo
     CDS             complement(11229..11804)
                     /gene="ves"
                     /locus_tag="QBF46_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
     gene            complement(12007..12492)
                     /gene="spy"
                     /locus_tag="QBF46_002632"
     CDS             complement(12007..12492)
                     /gene="spy"
                     /locus_tag="QBF46_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDQ6674796.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLNDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12822..13790)
                     /gene="astE"
                     /locus_tag="QBF46_002633"
     CDS             complement(12822..13790)
                     /gene="astE"
                     /locus_tag="QBF46_002633"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDQ6674797.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13783..15126)
                     /gene="astB"
                     /locus_tag="QBF46_002634"
     CDS             complement(13783..15126)
                     /gene="astB"
                     /locus_tag="QBF46_002634"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDQ6674798.1"
                     /translation="MNVWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQISNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQAQLLAN
                     LRARVNSFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMVLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(15123..16601)
                     /gene="astD"
                     /locus_tag="QBF46_002635"
     CDS             complement(15123..16601)
                     /gene="astD"
                     /locus_tag="QBF46_002635"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDQ6674799.1"
                     /translation="MTLWINGDWITGQGALRVRRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEVVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLVAMGGRTLLAPRLLQAGTSLLSPGIIEMTGVAGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGSSGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16598..17632)
                     /gene="astA"
                     /locus_tag="QBF46_002636"
     CDS             complement(16598..17632)
                     /gene="astA"
                     /locus_tag="QBF46_002636"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDQ6674800.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDCVRL
                     VRLCAEEKTA"
     gene            complement(17629..18849)
                     /gene="astC"
                     /locus_tag="QBF46_002637"
     CDS             complement(17629..18849)
                     /gene="astC"
                     /locus_tag="QBF46_002637"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDQ6674801.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19295..20101
                     /gene="xthA"
                     /locus_tag="QBF46_002638"
     CDS             19295..20101
                     /gene="xthA"
                     /locus_tag="QBF46_002638"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDQ6674802.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRS"
     gene            20258..20962
                     /locus_tag="QBF46_002639"
     CDS             20258..20962
                     /locus_tag="QBF46_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDQ6674803.1"
                     /translation="MKAECKFVVGSLTFTLAIYLVYASGIVNFINDLPHLQALIRQSG
                     IFGYSLYILLFVIATLLLLPGSVLVIGGGIIFGPLLGTLLSLVAATLTSSVSFLIARW
                     MGRDLVLKYVGDTTVFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTAIAFWP
                     YTFISTFTTLPGIFIYTFMASDLVTEGITLSFVFKICLAGLMLFTLIQLAKYYARYKQ
                     VTLTASDKTSLTEQHR"
     gene            20959..21663
                     /locus_tag="QBF46_002640"
     CDS             20959..21663
                     /locus_tag="QBF46_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDQ6674804.1"
                     /translation="MKDKNRKIWLWRGVIVVLLFASLLAWALLPGVHEFINRSVAAFA
                     AVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLSW
                     TSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPFD
                     PISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTVV
                     IFMAKKIWLERQKRNA"
     gene            21663..22211
                     /locus_tag="QBF46_002641"
     CDS             21663..22211
                     /locus_tag="QBF46_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDQ6674805.1"
                     /translation="MGLPPLSKIPLILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22221..23387
                     /locus_tag="QBF46_002642"
     CDS             22221..23387
                     /locus_tag="QBF46_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ6674806.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTVPLWQYLDVLHPYLWREGKDFPHSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23360..24895
                     /locus_tag="QBF46_002643"
     CDS             23360..24895
                     /locus_tag="QBF46_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6674807.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKWL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDIDQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGNTLAIFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFAVGIAQYMP
                     TLWQGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            24895..25548
                     /locus_tag="QBF46_002644"
     CDS             24895..25548
                     /locus_tag="QBF46_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDQ6674808.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAERQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            25615..26922
                     /gene="ynjE"
                     /locus_tag="QBF46_002645"
     CDS             25615..26922
                     /gene="ynjE"
                     /locus_tag="QBF46_002645"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDQ6674809.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLYEWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(26931..27551)
                     /locus_tag="QBF46_002646"
     CDS             complement(26931..27551)
                     /locus_tag="QBF46_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDQ6674810.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
BASE COUNT         6796 a         7313 c         6773 g         6710 t
ORIGIN      
        1 catattgtta acaaaattat tatctcattt aaatctaagt catttacaat ataagtttaa
       61 gagcgacgcc acaggatgaa ctatcaaaaa tagctcatca tgattagcaa aacttaacca
      121 ttttaaaata aataaacaat taaagaaaaa agatcactta tttatagcaa tagatcgtca
      181 aaggcagctt tttgttacag gtggtttgaa tgaatgtagc aacgaaatac agaatttcag
      241 gtcatgtaac tcccggcaaa accgggaggt atgtaatcct tactcagtca cttccccttc
      301 ctggcggatc tgatttgccc aacgttgggc agattcaggc acagtaaacg ccggtgagcg
      361 cagaaatgac tctcccatca gtacaaacgc aacatatttg ccacgcagca tccagacatc
      421 acgaaacgaa tccatcttta tcgcatgttc tggcggcgcg ggttccgtgc gtgggacata
      481 gctaataatc tggcggtttt gctggcggag cggtttcttc attactggct tcactaaacg
      541 catattaaaa atcagaaaaa ctgtagttta gccgatttag cccctgtacg tcccgctttg
      601 cgtgtatttc ataacaccgt ttccagaata gtctccgaag cgggatctgg ctggtggtct
      661 atagttagag agttttttga ccaaaacagc ggccctttca gtaataaatt aaggagacga
      721 gttcaatgtc gcaacataac gaaaagaacc cacatcagca ccagtcacca ctacacgatt
      781 ccagcgaagc gaaaccgggg atggactcac tggcacctga ggacggctct catcgtccag
      841 cggctgaacc aacaccgcca ggtgcacaac ctaccgcccc agggagcctg aaagcccctg
      901 atacgcgtaa cgaaaaactt aattctctgg aagacgtacg caaaggcagt gaaaattatg
      961 cgctgaccac taatcagggc gtgcgcatcg ccgacgatca aaactcactg cgtgccggta
     1021 gccgtggtcc aacgctgctg gaagatttta ttctgcgcga gaaaatcacc cactttgacc
     1081 atgagcgcat cccggaacgt attgttcatg cacgtggatc agcagctcac ggttatttcc
     1141 agccatataa aagcttaagc gatatcacca aagcggattt cctctcagat ccgaacaaaa
     1201 tcaccccagt atttgttagg ttctctaccg ttcagggtgg tgctggctct gccgataccg
     1261 tgcgtgatat ccgtggattt gccaccaaat tctataccga agagggtatt tttgaccttg
     1321 ttggcaataa cacgccaatc ttctttatcc aggatgcgca taaattcccc gattttgttc
     1381 atgcggtaaa accagaaccg cactgggcaa ttccacaagg gcaaagcgcc cacgatactt
     1441 tctgggatta tgtttctctg caacctgaaa ctctgcacaa cgtgatgtgg gcgatgtcgg
     1501 atcgcggcat cccccgcagt taccgcacca tggaaggctt tggtattcat accttccgcc
     1561 tgattaatgc cgaagggaag gcaacgtttg tacgtttcca ctggaaacca ctggcaggta
     1621 aagcctcact cgtttgggat gaagcacaaa aactaaccgg acgtgacccg gacttccacc
     1681 gccgcgagtt gtgggaagcc attgaagcag gcgattttcc ggaatacgaa ctgggcttcc
     1741 agttgattcc tgaagaagat gaattcaagt tcgacttcga tcttctcgat ccaaccaaac
     1801 tgatcccgga agaactggtg cccgttcagc gtgtcggcaa aatggtgctc aatcgcaatc
     1861 cggataactt ctttgccgaa aacgaacaag cagcattcca tccaggtcat attgttcctg
     1921 gtctggactt caccaacgat cccctgttgc agggacgttt attctcctat accgatacac
     1981 aaatcagtcg ccttggcggg ccaaatttcc atgagattcc gattaaccgt ccgacctgcc
     2041 cttaccacaa cttccagcgt gatggcatgc atcgtatggg gatcgacact aacccggcga
     2101 attacgaacc gaactcgatc aacgataact ggccgcgcga aacaccgccg ggaccgaaac
     2161 gcggcggttt tgaatcatat caggaacgcg tggaaggaaa taaagttcgc gagcgcagtc
     2221 catcttttgg cgaatattat tcccatccgc gtctgttctg gctaagtcag acgccattcg
     2281 agcagcgcca tattgtcgat ggtttcagtt ttgagttaag caaagtcgtt cgtccgtata
     2341 ttcgtgagcg cgttgttgac cagctggcgc atattgatct cactctggcc caggcggtgg
     2401 cgaaaaatct cggtatcgaa ctgactgacg accagctgaa tatcacccca cctccggacg
     2461 tcaacggtct gaaaaaggat ccatccttaa gtttgtacgc cattcctgac ggtgatgtga
     2521 aaggtcgcgt ggtagcgatt ttacttaatg atgaagtgag atcggcagac cttctggcca
     2581 ttctcaaggc cctgaaggcc aaaggcgttc atgccaaact gctctactcc cgaatgggtg
     2641 aagtgactgc ggatgacggt acggtgttgc ctatagccgc tacctttgcc ggtgcacctt
     2701 cgctgacggt cgatgcggtc attgtccctt gcggcaatat cgcggatatc gctgacaacg
     2761 gcgatgccaa ctactacctg atggaagcct acaaacacct taaaccgatt gcgctggcgg
     2821 gagacgcgcg caagtttaaa gcaacaatca aggtcgctga ccagggtgaa gaagggattg
     2881 tggaagctga cagcgctgac ggtagtttta tggatgaact gctaacgctg atggcagcac
     2941 accgcgtgtg gtcacgcatt cctaagattg acaaaattcc tgcctgatgg gaggcggcgc
     3001 aattgcgccg cctcaaatga tttacatagt gcgctttgtt tatgccggat gcggcgtgaa
     3061 cgccttatcc ggcctacaaa actgtgcaaa ttcaatatat tgcaggaaac acgtaggcct
     3121 gataagcgaa gcgcatcagg cagttttgcg tttgtcagca gtctcaagcg gcgcagttac
     3181 gccgcctttg taggaattaa tcgctggatg caaggttcac gccgcatctg gcaaacatcc
     3241 tcacttacac atcccgataa ctccccaacc gataaccacg ctgagcgata gcacctttca
     3301 acgacgctga tgtcaacaca tccagctccg ttaagcgtgg gaaacagtaa gcactctgac
     3361 ggatagtatt atcgataaac gccggatgac acatcacctc cagcgaacga tcaccccgat
     3421 ggcttgcgtc atccagcact tgcaggaaca gtgcctcgct aatctcttca ccataaaacg
     3481 cgctactgaa tccctgagaa ctgcgcagat tagccggtaa atcaccagag ttagatagcg
     3541 gctgacggtc gatgcgcaac gcaatcccct cttcagccgc aaacctggca acaatcggga
     3601 aaatctgcgg gaacatatgc acatgatgat ggctatcaag atgcgtaggt ttgcgtccaa
     3661 atagctcaat gaaacgcaaa tactgactgg caagctcctg agtaatttct tccagcggta
     3721 aagcatcttc ttctgccaac tgccagatcc attttcccag cacaccatcg cgggttaacc
     3781 ccggcatagc tgtcagtggc ttgcccatag taaggacaaa gtgcatccct atggccagac
     3841 tcggttcatc acgactcaac tgcaccgcat ggtcaatagc ctgcccattc accagcgccg
     3901 tcgtcgatgt gacaatccca ttgcgacagg cctcgataat gccgtagttc tgacctttgc
     3961 ttaagccaaa atcatcggca ttaacaatca gtaagcgttc cataatcagc ctcggttaat
     4021 gtgctttttt aagctctgcg atgcagtcgg caaaatttgg cagccatttc tcgtgcgcca
     4081 gaatcatctc gcgtgccagc agctcagcat cgcgatcgga atgcaccaac ggactaaggt
     4141 ttagcgccag taacacatcg ttaaattctc cgctaagtgc ggcgttgctg gcagcaatct
     4201 cgaagccttt aatggtgtga atcagcccca tcactttatc atcgaaatgc gtaatgcgcg
     4261 gatgtggcgt cgcgccatcg cgccccagct tacaggtcat ttctaccgcc cagtctgccg
     4321 gaatattatc catatgccca tgatgcggga tattaacgta atgttcagct tgcttgtcgt
     4381 tgtagatagc gttgatcact tcgcacgctg catcagagta ataagcccca ccgcgctgtt
     4441 ccagttcttt cggcttaact ttcaactccg gatttttata caactcaaaa agttgtttct
     4501 cgactttctg tactacctgt gctcgtgcgc cgcctttgta gtattcgccc atttcaatag
     4561 ccagcatctc tttctgcttg aagtaataca gcagataaga acatggcagc agatttaacg
     4621 aacgaattaa gccctcacta aatggcagat cgaaaatatt ttttacagag gacgctttta
     4681 actgccctga cgccacacca tcaagcaatt cggcaaagcg cgacttgcca tttaccagca
     4741 catccttaat gaacaccata tggttgaggc cgaacagatc gatagataaa tcatcgctgt
     4801 ctttcagcat cagaacatcg cgaataaaca tcttcatgcc aatcggaata ttacacacgc
     4861 cgataaagcg tttaaatcca gtatgacgat aaacggcttc agtgaccatt cccgccgggt
     4921 tagtgaagtt aatcacccat gcattcggac aaagttcttc gacatctttt acgatgtcaa
     4981 aaatcaccgg aatggtacgc agacctttaa acaaaccgcc cgcgccgttg gtttcctgac
     5041 caagataacc atgacttaat ggaatacgtt catccagttc acgcgccggt aattggccaa
     5101 cgcgcagttg ggtagtaacg aaatcagcat ctttcaatgc ttcgcggcga tccagcgttt
     5161 tataaagctt catcgggacg ccagcgttat caatcatccg ttggcagaga tcgaaaataa
     5221 tatccagttt agctttacca ccttcgacat ccaccagcca taattcgctg accggcaatt
     5281 cgtggtatcg cttaataaat ccttccagta actccggggt atagctgctc ccgccaccaa
     5341 tagtgacgac ttttaatttc tggctcataa tttctccctt cagtacagaa tactgatatc
     5401 tggcatatct gccccccgga cataaataat ccagcaacag gacagatatg tgaattgtca
     5461 ggtataacga cttactgatt aaattcagtc aatttcttac gatagctctt aggcgtaaag
     5521 gacgttaatt tcttaaacgt tttaataaac aaactcgggc tactataacc ggcctcaaac
     5581 gcaatatccg tcactgaata gttggtcatt tccagttgtt ttttggcaaa attaatacgg
     5641 atttcattaa taatctgcat tggcgttttg ccataatatc gttgagtcgc ccgcgtcaaa
     5701 tattcctgtg atttggctga caacgtcacc atattctcca gcgccgattc actaaactgc
     5761 tctttatcat gcatcttttc taccgtactt ttcagccact gcggtacatc atcaatcacc
     5821 tgttcttcgc ggtaatgacg taaacggtta atgacataaa aagtaaccat ctcaacaaac
     5881 tcttccagcc ctgtttcgcg gaaattcaat gaagaaatca ctgtttccac ataggtgaga
     5941 aaagcgttgt tggtccggta tacctgcgaa gctacaaagc aataaggcaa caacggcagg
     6001 taatgctgct caaaaaagcg tttactgatc ccaacgttca atatacgcgt ggcaccaaac
     6061 tcatagaaac tttgatggtg cgaacctaac ggaataaaaa caaaatcacc ccgttccagt
     6121 aacacgcgct taccgttaat ctcctggaaa taacgcccgg ttaataccag agtaaattca
     6181 taatagtcgt gctgatgcag tccgctgata ctctcagttt tgttatagat aaacacatgg
     6241 aagtttttgc cattaaacaa ctgctgttct cgggcagtgg caatttccgg cgcgttaatc
     6301 actggctgca tcatcgactc cttatgcctt cagtttttca tgaagctcaa ttaattcagt
     6361 aatcagttca cgcgcaagca tggacgtcat taaatgatcc tgagcgtgga ccagcaccag
     6421 actcactttc atcttacctt cgcccgcatc gccttcaatc agtttcgtct gtaccagatg
     6481 cgcttcattc aatgccagcc gtgactgatc catcatggct tttgctgcgg caaaatcgcc
     6541 ctgcttcgcc tgtttcagtg ccgcatacgc caggctgcgc gcttgtccgg agttgatgat
     6601 aagccccatc accacttctt ccagctcttc agcttccgtt tgcgtatcgg gaatgttgtc
     6661 gagatccatc atacatcgtt cctctctctg cttaccggca cgattacccg taccggcatc
     6721 gattaaaatt tcagggcgtt agcgatatct tcttcgctct cttctttatc aatcgcattt
     6781 tgtgctttgt tagccaccac aacaaagggc agataaatta acgttgcgat accaaggttg
     6841 aagagtgcga ccagcaatgc ggcgacgcta ccgttggtat taaagaaggc tcccagaccg
     6901 gttggcatgg tccacggtgc aatattggtc accggaggaa taatgcccat gtagtacgct
     6961 gcgagggtga ttgccgccag aatcggttgt accagtacaa acgggataaa catcaccggg
     7021 ttcatgataa ttggcagacc aaacagaatc ggttcgttaa tctggaagat gccggacggc
     7081 agcgccagtt ttgccacctg acgataatca gcacggcgag aggcgataaa gatagccagg
     7141 atcaggccta aagtcgcacc actgccccca aggaaaataa aggagtccag catcggcttg
     7201 gcccagatat ggaaggtctt accggccgcc agcgccgctt cgacggaacc atattgctga
     7261 taggtcgcga tattttccag cgcccacggc gtcataatgc cgttgtccag tgcggtcagc
     7321 gccagcgcgc catgaatacc gaagaaccag agcagtggaa caaagatcac ataggcccag
     7381 cccaccacgc tacccaacga tgccagtggg gttgagatgg tatccataat gatctgatgg
     7441 aagttggtgc cccaggtatt caacgcccag gcaataatcc ccatcacgga aagaataata
     7501 aagccgggaa ttaatgccga gaaggaacgc gatactgaag caggtacgct gtcgggcagt
     7561 ttaatgaccc aattgcggcg gacaataaag gtaaacattt ctgccaccac caggccaata
     7621 ataatcccgg agatgatatt cgccccacct aaccagtttg cgccaaccgc ataggcctca
     7681 ccgacactat atggggtgac ggtcataaat gctgcaacgg ataacaaccc cgccgccagc
     7741 gcatcgactt tacgctcttc tgccagcgcc atgccaataa agaacggtgc cattaaagac
     7801 attatcccta atgttccgtt atatacgttg ccgccaatac ctttcaggcc attaagtgtt
     7861 tcaatggttg aggcgtcgag gcgaatacct aaggaataaa aaaacgaccc ctccccaaag
     7921 cttagaaaaa cgttgttaat taatacaaac atggcccccg caagggttaa cggcattaag
     7981 cgaataaagc catttttgat tgcattaacg tgtggctgct ttcctatttt aactgcaaaa
     8041 gggaggagta ccttttcaag cgatgcaata acattactca tagaaaaata cccttaaaaa
     8101 ccgcaattta aatattgcgg tattgattta tgaaataact ctttgacggg aaaatttaaa
     8161 ataaattaat ttgctgcggc ttttttaatc gttgcaaccg cagccttaag cacgcctaaa
     8221 ccatcgactt tgccataaag cagcgagtca attacttcaa ccggtttgtt gggtaacaaa
     8281 cgctggattt cgggcaacat ataagcaatc tgcggcccta ataacacgac atcggcattc
     8341 tgaccttttt caccagccag tgtttccgga aatgcttcaa taatgaccgg aacttcatat
     8401 ttttctgcct gtgcgcgcat ttttgatacc agtaaagagg tagacatgcc cgcagaacaa
     8461 aacagataaa tgtgtttctt ttccataaaa ctgccctcat cgacgattat ctgtcagcca
     8521 gacactccgc aagccttaac ctgcttccat gctctgggta acttgcgaaa ccaacatgac
     8581 tttttttgtg gctgaaagga gtatacggta tagacctgag caatagtatt tttttgacct
     8641 tataaattgt ttctccttga cctgtggtta tgaccctctt cacatttcgg gcaaataatt
     8701 cgcgaagata attaagaact caggccaaaa aaaaccggcg caatggccgg tttccgttgt
     8761 tactcaagct ttcagacgaa ttgattactt cgcagcctgt ggatcagtgt cgtattcagc
     8821 acaggtctga taaccggagt tgatgacatg tccggtatca tctaacgcga caaagtaggt
     8881 ttctgcttta ccatcacgtt gacccaggat gtaggtctgg caagtaccgc gagcatggat
     8941 catgctcact tcagacgaag gtttacccgc aatttgtgca acctgcgccc ggctcatgcc
     9001 ttttttgacg tctttcacca caggctgtac aaactggtct ttggtacgat cataagccgt
     9061 acaacccgcc agcatggtta ataccgccgc tgcactcaga attcctgcca tattcttgtt
     9121 catattccgt cctcttgttt atcagcgtgt tagataagcc tggaatacat tgggcgcttt
     9181 ttcaagcccg tgaacgaaac ggctccgctt tcagaggatt cctgtatgac gttttaacca
     9241 ccattcagcc cgctgtcgct tgtcgtttca gtggcaacgg gttagcttta agaaagtttt
     9301 gtcttttctg tctggagggg ttcaatgaca ttgcaacaac aaataataaa ggcgctgggc
     9361 gcaaaaccgc agattaatgc tgaagaggaa attcgtcgta gtatcgattt tctgaaaagc
     9421 tacctgcaaa cttatccgtt cattaaatca ctggtgctcg ggatcagcgg cggtcaggac
     9481 tccacgcttg ccggaaagct gtgccagatg gcgattaatg agctgcgcca ggaaactggc
     9541 aacgaatcac tgcaatttat tgccgtacgc ctgccctatg gtgttcaggc tgacgaacaa
     9601 gattgccagg atgccattgc ctttattcaa ccggatcgcg tattaaccgt taatatcaag
     9661 ggcgcggtat tggccagcga acagtcattg cgggaagcag gcattgaact gagcgatttt
     9721 gtccgtggca atgaaaaagc gcgtgagcgg atgaaagcac aatatagcat tgcgggtatg
     9781 accagcggtg tcgtggtggg caccgatcat gcagcagaag ccattaccgg attcttcact
     9841 aaatatggtg acggcggtac agacattaat ccgctgtatc gtctcaacaa acgtcagggt
     9901 aaacagttac tggcggcatt aggttgcccg gaacaccttt ataagaaagc gccaacggcc
     9961 gatctggaag atgatcgccc ttctctgcca gatgaagtgg cactcggcgt gacctatgac
    10021 aatatcgacg actatctgga agggaaaaac gtacctgaac aggtcgccag aacaatagag
    10081 aactggtatc tgaaaaccga acataaacgc cgtccgccaa ttaccgtttt cgatgatttc
    10141 tggaaaaagt aataatttgc acataatggt ttgtgcgaaa aagagtaatt tgttcacgcc
    10201 ggatgcggcg tgaacgcctt attcgaccta caaaactatg caaattcaat atattgcagg
    10261 agtcgaatag gcctgacagg cgtagcacgt cagacggtgt aacctttgtc atcgacccgc
    10321 ctctttttta atcgctcccc gcctgttaca ctggatagat aaccagcatt cggagtcaac
    10381 agtggtacgg cgtttaactt ctccgcggct cgaatttgaa gctgcggcaa tttatgaata
    10441 tcccgaacat ttacgttcat tccttaatga cttacccacc cgaccagggg tgtatctgtt
    10501 tcatggcgaa agtgacacca tgccgctcta tatcggcaaa agcgttaata tccgcagtcg
    10561 ggtgctttct catttacgca ccccggatga agccgccatg ctacggcaat cccggcgaat
    10621 tagctggatc tgcaccgcag gcgaaatcgg cgctctgctc cttgaagcgc gattaatcaa
    10681 agaacaacag ccgctgttta ataaacggtt gcgccgcaat cgccagctct gtgccctgca
    10741 attaaatgaa aagcgcgtcg atgtggtgta tgccaaagag gtggattttt cacgagcccc
    10801 caacctgttt ggcctgtttg ccaataggcg cgcagctttg caagcattgc agagcatcgc
    10861 tgatgaacaa aaactttgtt atggcctgtt gggactggaa ccgttaagtc gcggtcgtgc
    10921 atgttttcgt tcagcgctaa aacgttgcgc cggagcatgc tgaggtaaag agagccatga
    10981 ggaacatgcg ctacgcttgc gccagtctct ggagcgtttg cgggtggtgt gttggccttg
    11041 gcaaggtgcg gtggcactga aagaacagca cccggaaatg actcaatatc atattattca
    11101 aaactggctg tggctggggg cggttaattc gctggaagaa gcgacaacgt taattcggac
    11161 acccgccggg tttgatcacg acggttataa aattctttgt aagccgctgc tttccggtaa
    11221 ctatgaaatt actgaacttg atccggcgaa tgaccagcga gccagttgat ttcactgaac
    11281 aacagtttac cttgcggttg taaaagccgc agggtgtgcc tgccatcgaa ccagcacgcg
    11341 ccctggtcgg tagtgagcaa tttatcgccc agttgtcacg ccccgttaat cacaaacacc
    11401 accccgccgc gtgagccaaa ggtagtaaac gtacgttcgg caattctgac tttcgcctta
    11461 cagacatcca ggcgtgtcat gatgttgaaa tccatcgaca tctgccctgc cgtcagtttc
    11521 gcttttacca cctggtccgc tgcaaaggca aaaggctgta acggttttaa ggtatggtta
    11581 aagcggtctg cgctttcaag gagcatctcg ccgccttcca gcaacgtcac tatcctttcc
    11641 atgccgggaa acaaagaaaa ctccccattc gccgcgatgg atgcaatgct ggcacgccag
    11701 taaaaatcac gttttgccgg tgggaaagtg caaatttcgc gcgtttcacc ggcagcattt
    11761 cgccacagat tcaccgacat tttacgcata tcaaagtatt ccatactcgc tcccggcccg
    11821 ttcttttcat tgttatcgtt caggtaaccg tggatggtta tcggcaactc gggtgattta
    11881 cgttagtggt gatcaggctt tttttacaac tgtttgagaa gagaaaagaa aaccgccgat
    11941 cctgtccacc gcattactgc aaggtagtgg acaagaccgg cggtcttaag ttttttggct
    12001 gaaagattat tcagcagttg caggcatttt accttttgcc gctggacgtt ctgtcagacg
    12061 cttctcaaaa ttagcattaa attgcttttt ctgttccggc gtcaggatgt tgtaaatttt
    12121 gttctgggtt tccatgtgcg ccagcatgtt agctttgcgc tgttcttcca tttttgcgat
    12181 ctgcgcttca gcttttgctt tatcgaaggt atcgctggca atgatgtcat gcattgcgcg
    12241 gcgttcttcc agcggcggac gtttcatctg gtcacgctgg cctttcatga tttcgcggat
    12301 ctgctgtttc tgcgcgtcgt tcaggttcag gtctttgaac atcatgtcct gatgcggacc
    12361 gaacttgcct ttgtggtgca tcatcggctt cgcgtcagcc ggtgctgcgg tagtggtgtc
    12421 tgcggcatgg gccaggttag ccgcgccaag agccagggta gaggcaacaa acagtgcagt
    12481 taatttacgc atattctata tccttccttt cagttattta ttacggcttt cttaagtagc
    12541 gtgccgtgtt gacgagatta actttactga ctttagcgtc aattaatcag agcaacggta
    12601 aaacaatgaa agtgtaaaaa acacttttgc gccaattatg gagaaaaaaa gaaaatttga
    12661 tggagagtga tgagagaata ttacaacacg atgattttgc agagattatg aagaactata
    12721 ccggatgact ggtgataaat aaagcaaata accaggatta atctgtatta atttataaga
    12781 aagcaactta atacccgcag aatgatttct gcgggtaagt attagcttat tttttcgagc
    12841 attaatcctg cgcgtaatcc caacgctacc aacggattag ggaataacac atactctaca
    12901 tcatgggtta cggtaaaacg ttcctccccg tcctgcgcca gcaatgttcc tttctcaaac
    12961 ggcataaaat tcagcgtgtc acttgccata tgcatttcga aggacggcga gtggcgagta
    13021 atttgcgaaa ccacccgata acggagcggc ggcgttctca cgataccgac actctcacca
    13081 gatagcagcg cagcaatggc gctggcagtt actgcaaact ggcgaagatc gttttgccca
    13141 aagggcaatg ctttgccaag ttccagcgta caggccagcg cgccaaaatg tctggcgctg
    13201 aaatgggtaa acgtaccacc aggctcctga tggaacacca gcgcctccag ccccgccgca
    13261 cccagccacg tcagaaattt ctcgtcccag ggaatgtcgc gttgcggtaa tacaccgaac
    13321 tgcggatgca aggagccacg aattgcggta tgtagatcaa ggtgccagcg cacagattct
    13381 ttaccctggt cataaaaatc ttccaggcac tgttccagtt cgcgcgcccg acaggtttcc
    13441 ccgctttcag caaacagctg ccaacgaccg ccaaacattc ggttcatatc gctatggcaa
    13501 taacgtttcc cttgcttcag cgcaggagga ttcccgagga tcaccagcaa ccgccaacgt
    13561 aacgggatct cgccgtgaga tatcgcgcca agcaacgcgt ccagcatctc cacaggtgca
    13621 gtctcattac cgtgtattcc cgctgaaatc accagcgcgc cttgcggtgg cgttaatggc
    13681 gtcagttcca gcacaccatc gcccagccag cgccagcgaa cgccgttgat ttctcgctcg
    13741 gtgataaccg gttttttacc cgttaaggtc agagcaagaa aattatccat tgccccctcc
    13801 ctcgcgctgg aacggataaa ccgaaccgag attcagtaat tggctcaata catccagtgc
    13861 ttcccgccct tcgcgcagca attgcgggtc ggccagatcg gcagcagtaa gacgatcgcg
    13921 gtagtaacga tccacccagt cattgagcgc attaaacagc gtatcgttca tcatcaccgc
    13981 cggattcacc gcccggcgtt cttcttctgt caataccacc cgcaaccgca ggcacgccgg
    14041 gccgccgcca ttcgccatgc tttcacgtaa atcaaagact tttagttcgc taatcgggtt
    14101 gtcagcggca aggagttcat tgagataacc ccatactccg gcgtgttccc gacactcctg
    14161 tggcagcacc aacaccatgg aaccatcatc gcggctcagc agttggctgt tgaacagata
    14221 tgtagacacc gcatccgaca cggaaacctg cgccgtgggc acctctattg ccataaagct
    14281 attgacccga gcacgcaggt ttgccagtaa ctgcgcctgt cgagcgaacg cctgttgatg
    14341 acaaaacagc acctggcggt tactcacggc aatcacgtca ttatgaaaaa cgccctggtc
    14401 gataacgtcc gggttttgct gggcgaaaat cacctgttgg ggattcacct gattcagcct
    14461 tgccaccgcc tcgctggctt cgcgagtctg tcgcgccgga taacgggaag gccgggtatc
    14521 attgccctct tctcgcccgt agacaaaaag ttgcataccc ggttcaccgt aatgaccgcc
    14581 gagacgattg tggtttgccg ccccctcatc accgagcaac gctacctgcg gcaacgccga
    14641 atggacacta aatttctctt cgtcgttaaa aatcgctttt aaaagtgatt cagtgacggg
    14701 ggcttccagc gaacggtgaa atttattgtt caggttggca actgtgagat gcactttgcc
    14761 atccagcgta tcggcagatg gcgcgatcgt cgccgcattg gctacccaca ttggcgaagc
    14821 ggagctgacg ctggaaagcc agtgtggtgc ctggcgtgca actttttcca gtacctgctc
    14881 atcgctacca ctgaatccca actgacgcag caccggaata aacggacgct cgtgcggcgg
    14941 gatcaccgcc tgggggaatc ccgcatcggc aagggctttc attttcagta agccctgttt
    15001 tgccgccagt cgcgggttag agatctgaaa acggtgacgg gtagaggctt cattaccaaa
    15061 cgacaagcca gcgtaatgat gcgtcagccc taccagcccg tcgaaattaa cttcccagac
    15121 gttcatcgca ccacctcatc ggaaaaatcc agtccggggt taagcgtggc gggtaacgtt
    15181 aacgagtccg actccaggct cgccatcggc caggcacaat aatctgcggc ataccaggcg
    15241 ctggggcgat ggttgccgga agagccaatg ccgccgaatg gcgcggtact ggcagcacca
    15301 gtaagcggtt tgttccagtt aacaatcccc gcccgcgcct ccagcaacag ttgatcgaat
    15361 ttttcccgct cgggggaaac cagaccgcaa gagagtccga agcgagtgtt attcgccatt
    15421 cgaatcgctt catcgaaagt atcataacgc cagacgcgca ataacggtcc gaacacctct
    15481 tcatctggta cgccagcaac gcctgtcatt tcaatgatcc ccggcgacag caacgatgtc
    15541 cctgcttgta ataagcgcgg cgcaagcagg gttcgtccgc ccatcgctac cagttgctgc
    15601 caggcagtaa ccacctgctg tgcggcctgt tcagaaatca gcccgccaat aaacggctgc
    15661 ggttcgtcat cccagttgcc cggcgttaat cgctggctga cggcaaccag acgagcaaga
    15721 aacgcatcgc cctgcgcccc gcttttcagc aataaacggc gggcgcaggt gcagcgttga
    15781 ccggctgtga caaacgccga ctgaatggtc agatggacag cggcatcgat atccgccacc
    15841 tcatcgataa ttagcggatt attaccgccc atctcaaggg cgagaatttt ctccggctga
    15901 ccggagagct ggcgatgcag ctggtagccg gtattggcgc taccggtaaa cagcaaaccg
    15961 tcgagatcct ccagcgcact cagcgcctga cccgtttcac gcccgccctg taccaggttc
    16021 agtacacctg gcggcaagcc agcctgctgc cataaacgca ttaccacttc gccactccac
    16081 ggtgtcagtt cgctgggttt aaagataatg gtgttacctg ccagcaatgc cggaacgata
    16141 tgtccattcg gcaaatgacc agggaaatta tacggcccaa acaccgccag cacgccgtgc
    16201 gggcgatgtc gcaggctcgc cgcgccgtcc ggcatttcgc tacgctgctc gccggtacga
    16261 acgtgatacg ccttaattga tatcgcgatt ttattgatca tcgccgtcac ttcggttgcc
    16321 gcttcccagc gcggcttgcc ggtttctcgg gcaatgattg cggttaattc gcctttattc
    16381 ctttccagca atccggcaaa gcgttcgaca acggcctgac gttcagcaaa tgagagccgc
    16441 gcccagcgag gaaacgccgc acgggctgcc cgacaagcct gctcgacctg agcggcatcg
    16501 gcatcattgc cctgccataa cacctcgccc gataccggat tacgcctcac acgcaatgcg
    16561 ccctggcccg ttatccagtc accgttaatc cataaagtca tgctgttttc tcctctgcgc
    16621 acaggcgcac cagacgaacg caatccccgg cgtggcattt cagggcatcc agttgtgcgg
    16681 cggttaatat caaacgctcg gttgccggat cggtacgcac cagcaccacg cggaaatggt
    16741 gataattttc attggcgacc aggcaggccg gaaaatcgcc ctgcgcaggc tgcccttctg
    16801 ccacttccac cagccgactt ttacggatgg cgcgcacgcg gtcgatgtca cactcaagcg
    16861 ttggcccgcc gtcgaagatg tcgatatagt tgcggtaacg aaaaccttct ttctccagca
    16921 ccgcgcgggc aggcgcggtt tgcggatgta cctgaccgat gacgtcctgg gcttcctggg
    16981 ataaaaagtg ggtatagatc ggatgtttcg gcatcagttc tgcaataaat gccttttgcc
    17041 cggtgccgca gagaaaatcg gcgcggctaa aatccatcga aaagaagcgt ttaccgaggc
    17101 tttgccagaa cggtgaatag ccgtgttcgt caatcacccc gcgcatttca gcaaccactt
    17161 tgtcattaaa cttgtcgcga aaagccgcca taaacataaa gcgcgatttc gacagcaaat
    17221 agccgttgcc ctctttgcgc cagtccgggt cgagaaacag cgtgcacagc tcgctgctgc
    17281 cggtgtgatc gttactgaga aacagcgtcg gcaatgcgtt atagacattc agctcttttg
    17341 aggcgtgaac caacgtgccg acgcgatagt tgtaccaggg atcgttcagc ccaaccgcca
    17401 cctcaatggc acaaatcccc gccacggtgc ctgtctcgct atcttccagc acgaacacat
    17461 agccctgctc acttttgggc agttcgcctt gccaggtttt gattgccctt tcgatacgcg
    17521 ccgaaagcgt ggcttcattg gcgggaagcg acgtcaggcc gccgcccgtt ttgctggcaa
    17581 gctgcatcag cgccgagaca tctgatcgct caacgggacg gatgaccatc atgatgaacc
    17641 tcggctaaca aagtgttcgc aagcagctgc aaagcgatcc agtccggtcg tcacctcttc
    17701 ttcgctgaca ttgagcgcag gcgcaaaacg caccacgttg ccacccgcaa tcagtaccat
    17761 cacgcctgct ttcgccgctt cctgagagat ctgtttcgct tgcccagcgt aatcggcatt
    17821 cagtacacag ccaatcagca aacctaagcc gcgaacttca ctgaacaaac catagcggtg
    17881 attaatagta ttaagacgct caacaaacca gtcgtgacgc tgtttaacgc cattaagcat
    17941 ctctggtgtg ttgatgagct ccagcacttt gcctgccacc gccgaggcca gcggattacc
    18001 gccataggtg gtgccatgag tgccaacggt catcacgcgg gcgcactctt cggttgccaa
    18061 caacgcaccg accgggaaac cgccgcccag cgctttggcg gtagttaaca gatcaggcgt
    18121 cacgccgtag tgcatatagg catacagttc cccggtgcgc ccgacgccgg tttgtacttc
    18181 atcaaaaatc aacagcgcat tgtggcggtt acacaattca cgcagacctt gtaaaaacgc
    18241 gttgctggct ggcaccacac cgccttcccc ctggatgggt tcgacaatca ccgcacaggt
    18301 agagtcgtca atcagcgcgc tggcagagtt aatatcgtta tatgcagcat gacgaatatc
    18361 cgccggcagt ggcgcaaaat cctgtgaata ggctggctgc ccacccgcac tgacagtaaa
    18421 cagcgtgcga ccatgaaacg catttttgaa cgccacgatg ccgctcttat ggctgccgta
    18481 gcggtcgtga gcgaatttac gcgccagttt tagcgccgct tcgttggctt ccgcaccgga
    18541 gttacaaaag aagacgcgat cggcaaacgt ggcgtcgatc aattttttcg ccagtcgcag
    18601 taccggctcg ttggtgtaac cgttgccggt atgccagaac ttactcgcct gttcgttcag
    18661 cgcttcacgc agttccggat gcgcatggcc cagcgcgttc accgcaatgc cacccgcgaa
    18721 gtcgatatac tctttcccct gctgatccca caagcgcgaa ccttcgccac gtaccggtat
    18781 aaagggtgcc ggagcgtaaa caggtatcat ccattcatca aagttttcac gcgtaattgg
    18841 ctgagacata gcgacctcta cagtaaataa ttcgttattt atatgttaat aataagtaat
    18901 gtttgcgctg taaatgtaga ttgcagggtt cgtgccagcc agtgataaaa gtgcataaac
    18961 ggcggaggct aactggaaat caaggagtta taaccaaacc atatgcattt aaagtgcata
    19021 taaagtgaat acgtttgcga tgtgggtgaa taaaaagaat aaaaaacgca atgttatgca
    19081 gaagtaaaat ataattctgg aattgtgatc attgacgaaa tttactggaa attactgcgc
    19141 cattctgacg cagcgcgcac caaaagcggg cattttttgc gccatcgttg acatcattaa
    19201 caaccatcga tcaaatcact taacaacagg cggtaagcaa cgcgaaattc tgctaccatc
    19261 cacgcactct ttatctgaat aaatggcagc gactatgaaa tttgtctctt ttaatatcaa
    19321 cggcctgcgc gccagacctc accagcttga agccatcgtc gaaaagcacc aaccggatgt
    19381 gattggcctg caggagacaa aagttcatga cgatatgttt ccgctcgaag aggtggcgaa
    19441 gctcggctac aacgtgtttt atcacgggca gaaaggccat tatggcgtgg cgctgctgac
    19501 caaagagacg ccgattgccg tgcgtcgcgg ctttcccggt gacgacgaag aggcgcagcg
    19561 gcggattatt atggcggaaa tcccctcact gctgggtaat gtcaccgtga tcaacggtta
    19621 cttcccgcag ggtgaaagcc gcgaccatcc gataaaattc ccggcaaaag cgcagtttta
    19681 tcagaatctg caaaactacc tggaaaccga actcaaacgt gataatccgg tactgattat
    19741 gggcgatatg aatatcagcc ctacagatct ggatatcggc attggcgaag aaaaccgtaa
    19801 gcgctggctg cgtaccggta aatgctcttt cctgccggaa gagcgcgaat ggatggacag
    19861 gctgatgagc tgggggttgg tcgatacctt ccgccatgct aatccgcaaa cagcagatcg
    19921 tttctcatgg tttgattacc gctcaaaagg ttttgacgat aaccgtggtc tgcgcatcga
    19981 cctgctgctc gccagccaac cgctggcaga atgttgcgta gaaaccggca tcgactatga
    20041 aattcgcagc atggaaaaac catcagatca cgcaccggtg tgggcgacct tccgcagcta
    20101 atgacgcagc ctcctgacat tctcccggtc agggggccga ttcttgagga taatcaagaa
    20161 actggcagta aggatttgtg accagccagg gtttgttata ccgtcatcgc gtttttcact
    20221 tctctattat caaggcttga tataggaaat ctccacgatg aaagctgagt gtaagttcgt
    20281 tgttggtagc ctgactttta ctctggcaat ttatctcgtt tatgcttcag gcatagtcaa
    20341 tttcattaac gatcttcctc acttgcaggc acttattcgc cagagtggta ttttcggtta
    20401 cagcctctat attctgttat ttgttattgc cactcttctg ctgctacccg gtagtgtcct
    20461 ggtcattggc ggtggcatta tttttggtcc actattgggg actctgcttt cgttagttgc
    20521 agcaacgctc acttcgtcag tttccttttt gattgctcgc tggatggggc gagatctggt
    20581 gctgaaatat gttggggata ccaccgtctt tcaggctatt gagaaaggga ttgcacgtaa
    20641 cggtatcgat tttcttattc ttacccgctt aattccgcta tttccttaca atattcaaaa
    20701 ttacgcctac ggattaaccg ccattgcctt ttggccttat acctttattt cgacattcac
    20761 tactctgccg gggatcttta tttatacttt tatggccagc gaccttgtta ctgaaggcat
    20821 aacattatca tttgttttta aaatttgtct ggcagggcta atgctattta ctcttattca
    20881 gcttgcaaaa tactacgccc gttataaaca agtgactctg accgcttctg ataaaacctc
    20941 tttaaccgaa caacatagat gaaagataaa aaccgtaaaa tctggctctg gcgtggcgtt
    21001 attgtcgtct tgttgtttgc ttcgctgctc gcatgggcgc tgcttcccgg cgtccatgag
    21061 tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat agaacgcttt
    21121 attcagtctt acggcgcact ggcggcagtt gtctcgttct tgttgatgat tttgcaggcc
    21181 attgctgcac cgctacctgc gtttttgata acctttgcca atgcgtcgct gtttggcgcg
    21241 ttctggggcg gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct gtgctttttt
    21301 atcgccagag tgatgggccg cgaagtggtg gaaaaattaa ccggcaaaac cgtacttgac
    21361 agtatggacg gctttttcac tcgctacggc aaacatacca tcctggtctg tcggttattg
    21421 ccttttgtcc ctttcgatcc aatcagctat gctgccggtt tgacttcaat acgttttcgc
    21481 tcgtttttta tcgccaccgg gcttggtcag ttaccggcaa ctattgttta ttcctgggcg
    21541 ggcagcatgt taacaggcgg tactttctgg tttgtcaccg gactgtttat tctgtttgcc
    21601 ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg agcgccagaa gaggaatgcc
    21661 tgatgggttt accgccgctt agcaaaattc ctttaatttt acgtccacag gcgtggctgc
    21721 atcgtcgcca ttacggcgag gtgctaagcc ccattcgctg gtgggggcgg atcccgttta
    21781 tcttttatct ggtgtcgatg tttgttggct ggctggagcg caaacgctca ccgctcgatc
    21841 cggtagtacg atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgtg
    21901 tggatatcac cagtatgaaa gtcgccgagc gcaccggcag cagcgataaa ctgctggcag
    21961 tggctgactg gcggcaaagc ccgctcttta gcgatgaaga acggctggcg ctggagtacg
    22021 ccgaagccgc aagcgtaacg ccgccaacgg tcgatgatgc cctgcgtacc cgactggctg
    22081 cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg caaaatctgt
    22141 cagcccgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt attccggaaa
    22201 aacgttctta aggagagatg atgcgccatt gtgggtggtt gctgggattg ttatcgctgt
    22261 tttctctggc aacacatgcc agtgactggc aggaaattaa aaatgaggcc aaagggcaaa
    22321 ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc gactgggtta
    22381 gcggcgagat gaaaacccat tacgctataa acctgaagat tgtccgtctg gcggatgccg
    22441 cagacgcggt gaagcgcatt cagaccgaag ccgcagccgg gcgcaaaacg ggcggctcgg
    22501 tggatctgct ctgggtgaac ggcgaaaact tccgcacctt aaaagaggcc aatttattac
    22561 aaacgggctg ggcggagact ctgcccaact ggcgctatgt cgacacacag ctgccggtgc
    22621 gggaagattt ttcagtgccg acacaaggtg cggaatcgcc ctgggggggc gcacaactga
    22681 catttatcgc ccgccgcgat gttacgccac agccaccaca aacgccgcaa gccttactgg
    22741 agtttgctaa agccaatccc ggcacggtta cctatccgcg cccaccggac tttaccggca
    22801 cggcgtttct tgaacagttg ctgattatgc tgacgcccga tcccgccgca ttaaaagaag
    22861 cgccggacga tgcgaccttc gcccgtgtca ctgtgccatt gtggcaatat cttgatgtgc
    22921 tgcatccgta tttgtggcgc gaaggaaagg atttcccgca ttcgcccgcg cggatggatg
    22981 ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt taaccccgcg catgcgcagc
    23041 aaaaaatcgc cagcggcgat ttgcctgcaa gcagttacag ttttggcttt cgcgagggga
    23101 tgattggcaa cgtgcatttc gtcaccattc ctgccaacgc gaatgccagt gctgcggcga
    23161 aggtagttgc caatttcctg ctctcacccg atgcgcaact gcgtaaagca gatcccgctg
    23221 tctggggcga tccttctgtt ctcgatccgc aaaaactgcc tgacgggcag cgcgaatcat
    23281 tgcaatcaag aatgccgcag gatctgccgc cggtactggc tgaaccgcac gcaggttggg
    23341 taaatgcgct ggaacaagaa tggctacacc gttacggtac gcattaatct ttttgctgtg
    23401 ggcgatggtg gcggtgattt atgcaccgct gatcccggca gctctcacgc tgatatcgcc
    23461 tgctttgtcg ttgacacact ggcaggcgtt atttgccgat ccacagttac cgcaggcatt
    23521 actggcaacg ctggtgtcga caaccatcgc ggcggtcggg gcattgttga ttgccctgct
    23581 ggtgattgtg gcgctgtggc ctgggccgaa atggcagcgt atgtgcgccc gtctgccgtg
    23641 gctgctcgcc attccccatg tggcttttgc caccagcgcc cttctgctct ttgctgacgg
    23701 agggctgctt tatgactatt tcccgtattt cactccgcca atggaccgat ttggcatcgg
    23761 tctgggcctc acccttgcag tgaaagaaag cgcatttctg ctgtggatct tagcagcagt
    23821 attgagcgaa aaatggctgt tgcagcaggt cattgtgctg gattcgctgg gctacagccg
    23881 ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg cctgcgctgg caatggcgat
    23941 gctggcgatt gttgcctggt cgctgtcggt cgtggatgtg gcaattattc tcgggccagg
    24001 taatcccccg acgctggcgg taattagctg gcagtggtta acccagggcg acattgacca
    24061 acaaacaaaa ggcgcgcttg ccagcctgct gttgatgctg ttactcgccg cctacgtttt
    24121 gctgagctat ctgctatggc gcagctggcg gcgcactatt ccccgcgtag atggcgttcg
    24181 caagcctgcc acgcctttat tgccaggcaa tacgctggcg atttttttac ccttaaccgg
    24241 tgtgctgtgt gtggttctgc tggcgatcct cgcggatcag tcgacgatca atagtgaagc
    24301 gctcatcaac agcctgacaa tggggctggt ggcgacattc atcgctttgc tcctgttact
    24361 gctgtggctg gaatgggggc cacagcgtcg ccagttgtgg ctatggttgc ccattttatt
    24421 acccgctctg ccactggtgg cgggccagta cacgctggcg ctatggctga acctggatgg
    24481 aagctggacg gcggtggtct gggggcatct gctgtgggta atgccatgga tgctgtttat
    24541 cctgcaaccc gcctggcagc gcattgattc acggttaatt ttgattgcgc aaacactggg
    24601 ctggtcgcgg gccaaaatct tcttttacgt gaaatgtccg cttatgctac gccctacgct
    24661 gattgccttc gcggtgggat ttgcagtcgg tattgcgcag tatatgccaa cgctgtggca
    24721 gggcgcgggg cgttttccga cgctcaccac tgaggcggtg gcattaagca gcggcggcag
    24781 caacggtatt ctcgccgccc aggctttatg gcaactgcta ttaccgctta ttatttttgc
    24841 cctgaccgcg ttagtcgcaa aatgggtagg ttatgtcaga caaggactcc gctaatgctc
    24901 tgcgtgaaaa atgtttcgct acgtttacca gaaagccgct tgctgacaaa cgttaacttt
    24961 acggtggata aaggtgacat tgtcacgtta atggggccgt ctggctgtgg aaaatccact
    25021 ctgttttcat ggatgattgg tgcactggcc ggacagtttt cttgtacagg tgagctatgg
    25081 ctcaatgagc aacggattga catgctaccc accgcagagc gtcagattgg cattcttttt
    25141 caggatgcac tgttatttga ccagttcagc gtcgggcaaa atttactgct ggcgctaccg
    25201 gcgacactta aggggaatgc ccgacgtaat gccgtgaatg atgcacttga gcgttcaggc
    25261 cttgacggag ctttccatca ggatcctgcc actttgtctg gcggtcagcg agcgcgcgtt
    25321 gctctgcttc gcgcccttct cgctcagcca aaagcgttgc tactggatga gccattcagc
    25381 cgtcttgatg tggctctgcg cgataatttt cgccagtggg tgttcagcga ggttcgcgcc
    25441 ctggcgatcc ccgtcgttca ggtaacgcac gatctccagg atgttcctgc tgatagttct
    25501 gttctggata tggcgcagtg gtcagaaaat tacaacaaac tgcgataacg caaagttttt
    25561 ctcaatgcgt cagttcagaa tggcgcactc aaaactacaa tgtcgggatt ttcgatgaaa
    25621 cgtgtttctc aaatgaccgc gctggcaatg gctttagggc tggcttgcgc ttcttcgtgg
    25681 gccgctgaac tggcgaagcc tcttacactt gaccagcttc aacaacaaaa tggcaaagcg
    25741 atagatactc gccccagcgc gttttataac ggctggccac aaaccttaaa tggcccttct
    25801 ggtcatgaac ctgccgcctt aaacctctct gccagctggc tcgacaaaat gagcaccgaa
    25861 cagctctatg agtggatcaa gcaacataat ctgaaaaccg atgctccggt ggcgctgtac
    25921 ggcaatgaca aagatgtcga cgccgtcaaa acgcgtctgc aaaaagcagg ttttacgcat
    25981 atctccattc tgagtgacgc gctaagcgaa ccttcccgtc tacaaaaact gccgcacttt
    26041 gagcagctgg tttatccgca atggctgcac gacctgcaac aaggtaaaga ggttacggcg
    26101 aaacctgccg gtgactggaa agtcattgaa gcggcctggg gcgctcctaa gctttacctt
    26161 atcagccata ttcccggtgc tgactacatc gataccaacg aagtggaaag cgaaccgctg
    26221 tggaacaaag tttctgatga acaactaaaa gcgatgctgg caaaacacgg cattcgccat
    26281 gacaccacgg tcattctgta cgggcgtgac gtatacgctg gagcgcgtgt ggcgcaaatt
    26341 atgctctacg caggcgtgaa agatgtgcgt ctgctggacg gcggctggca aacctggtcc
    26401 gacgcgggac tgcctgttga gcgaggaacg ccaccgaaag tgaaagcgga accggatttc
    26461 ggcgtgaaga tcccggcaca accgcagttg atgctcgata tggaacaggc ccgtggactg
    26521 ctgcatcgcc aggatgcatc gctggtgagc attcgttcgt ggccagaatt tatcggtacg
    26581 accagcggtt acagctatat taaaccaaaa ggtgaaatag ccggcgcacg ttggggacac
    26641 gctggtagcg actcgacgca tatggaagat tttcataacc cggatggcac catgcgcagc
    26701 gccgatgata ttaccgctat gtggaaagcg tggaatatca aaccagagca gcaagtttca
    26761 ttctactgcg gcaccggctg gcgcgcgtcc gaaaccttta tgtacgcacg cgccatgggt
    26821 tggaagaatg tttccgtgta tgacggcggc tggtacgaat ggagcagcga tccaaaaaat
    26881 ccggtagcaa ccggtgaacg cggcccggac agtagcaaat aacattgcaa ttactgacgc
    26941 tggagcgact tcagcgtcag atacccactc cacacccgcg taaaggtcgt catccagcac
    27001 aacgcaccga agatccacgc aaaccacgca aaccatgcag ggaataaaca tcccagcaca
    27061 aacagtaaga tcgtttctgt gccttcggtt aatcccccca gatagtaaaa cgacttatgt
    27121 gcataaccgg ggttatcaat ctgatgtttt gctgccagtg cagcaaaggc gagaaaactg
    27181 ctgcccgtgc cgataaacgc aaacaacaac cagccgcctg ccagcgcatt ttgctccggt
    27241 gcagcaagaa taaagccaaa cggcaccagc gcgtaaaaga gaaaatcgag agaaatatca
    27301 agaaagccgc ccgcgtcggt aagctctctg cgccgcgcca gcgcaccgtc cagaccatca
    27361 agcagcctgt tcaacaaaat gacgactagc gccgccagat accagcccag cgccagaaac
    27421 ggcagagcca gcacgccgat ggcaaatccc actaatgtta aaccatctgg cgtaatgccc
    27481 ggtttatcaa gaacccgcac gcattgatgc aacaacggtt taatccgggg atgaagatgg
    27541 cggtctagca caggcactcc ttaaatataa agcctttctg attgagcaac ag
//
LOCUS       DAOFQD010000062        27059 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.062, whole genome shotgun sequence.
ACCESSION   DAOFQD010000062 DAOFQD010000000
VERSION     DAOFQD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.062"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(22..828)
                     /gene="trpA"
                     /locus_tag="QBF46_002647"
     CDS             complement(22..828)
                     /gene="trpA"
                     /locus_tag="QBF46_002647"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDQ6674811.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(828..2021)
                     /gene="trpB"
                     /locus_tag="QBF46_002648"
     CDS             complement(828..2021)
                     /gene="trpB"
                     /locus_tag="QBF46_002648"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDQ6674812.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2033..3394)
                     /gene="trpCF"
                     /locus_tag="QBF46_002649"
     CDS             complement(2033..3394)
                     /gene="trpCF"
                     /locus_tag="QBF46_002649"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDQ6674813.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3395..4990)
                     /gene="trpD"
                     /locus_tag="QBF46_002650"
     CDS             complement(3395..4990)
                     /gene="trpD"
                     /locus_tag="QBF46_002650"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDQ6674814.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(4990..6552)
                     /gene="trpE"
                     /locus_tag="QBF46_002651"
     CDS             complement(4990..6552)
                     /gene="trpE"
                     /locus_tag="QBF46_002651"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDQ6674815.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTAFFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6574..6667)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6644..6688)
                     /gene="trpL"
                     /locus_tag="QBF46_002652"
     CDS             complement(6644..6688)
                     /gene="trpL"
                     /locus_tag="QBF46_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDQ6674816.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6826..7707
                     /gene="yciV"
                     /locus_tag="QBF46_002653"
     CDS             6826..7707
                     /gene="yciV"
                     /locus_tag="QBF46_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDQ6674817.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7704..8324
                     /locus_tag="QBF46_002654"
     CDS             7704..8324
                     /locus_tag="QBF46_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDQ6674818.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8352..10247
                     /locus_tag="QBF46_002655"
     CDS             8352..10247
                     /locus_tag="QBF46_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HDQ6674819.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENTEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMIIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLQFSNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNASAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILIRV
                     NEGATRNNEVLLPAEQLLLEALFRKCDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFTAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQTHLSCGAYTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            10460..11332
                     /gene="rluB"
                     /locus_tag="QBF46_002656"
     CDS             10460..11332
                     /gene="rluB"
                     /locus_tag="QBF46_002656"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDQ6674820.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11372..11962)
                     /gene="cobO"
                     /locus_tag="QBF46_002657"
     CDS             complement(11372..11962)
                     /gene="cobO"
                     /locus_tag="QBF46_002657"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDQ6674821.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(11959..12717)
                     /locus_tag="QBF46_002658"
     CDS             complement(11959..12717)
                     /locus_tag="QBF46_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDQ6674822.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12937..13986
                     /gene="sohB"
                     /locus_tag="QBF46_002659"
     CDS             12937..13986
                     /gene="sohB"
                     /locus_tag="QBF46_002659"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDQ6674823.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(14022..14273)
                     /locus_tag="QBF46_002660"
     CDS             complement(14022..14273)
                     /locus_tag="QBF46_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDQ6674824.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14653..17250
                     /gene="topA"
                     /locus_tag="QBF46_002661"
     CDS             14653..17250
                     /gene="topA"
                     /locus_tag="QBF46_002661"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDQ6674825.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            17461..18435
                     /gene="cysB"
                     /locus_tag="QBF46_002662"
     CDS             17461..18435
                     /gene="cysB"
                     /locus_tag="QBF46_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDQ6674826.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            18730..18894
                     /locus_tag="QBF46_002663"
     CDS             18730..18894
                     /locus_tag="QBF46_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDQ6674827.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            18897..19064
                     /locus_tag="QBF46_002664"
     CDS             18897..19064
                     /locus_tag="QBF46_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674828.1"
                     /translation="MVGQEQLESSPLCQDSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            19178..19273
                     /locus_tag="QBF46_002665"
     CDS             19178..19273
                     /locus_tag="QBF46_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674829.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            19437..22112
                     /gene="acnA"
                     /locus_tag="QBF46_002666"
     CDS             19437..22112
                     /gene="acnA"
                     /locus_tag="QBF46_002666"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDQ6674830.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTMGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKGIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(22176..22766)
                     /gene="ribA"
                     /locus_tag="QBF46_002667"
     CDS             complement(22176..22766)
                     /gene="ribA"
                     /locus_tag="QBF46_002667"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDQ6674831.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            22936..23700
                     /gene="pgpB"
                     /locus_tag="QBF46_002668"
     CDS             22936..23700
                     /gene="pgpB"
                     /locus_tag="QBF46_002668"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDQ6674832.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            23849..24157
                     /gene="lapA"
                     /locus_tag="QBF46_002669"
     CDS             23849..24157
                     /gene="lapA"
                     /locus_tag="QBF46_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDQ6674833.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            24164..25333
                     /gene="lapB"
                     /locus_tag="QBF46_002670"
     CDS             24164..25333
                     /gene="lapB"
                     /locus_tag="QBF46_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDQ6674834.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            25527..26264
                     /gene="pyrF"
                     /locus_tag="QBF46_002671"
     CDS             25527..26264
                     /gene="pyrF"
                     /locus_tag="QBF46_002671"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDQ6674835.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            26264..26590
                     /gene="yciH"
                     /locus_tag="QBF46_002672"
     CDS             26264..26590
                     /gene="yciH"
                     /locus_tag="QBF46_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDQ6674836.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(26716..26934)
                     /gene="osmB"
                     /locus_tag="QBF46_002673"
     CDS             complement(26716..26934)
                     /gene="osmB"
                     /locus_tag="QBF46_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDQ6674837.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         6614 a         6615 c         7144 g         6686 t
ORIGIN      
        1 gcggaactgg cggctgtggg attaactgcg cgtcgccgct ttcatcggtt gtacaaaaac
       61 tttcagtgcc gccagcattt tctctggctc attaatatgt tgctcgatga ttttaacaat
      121 ggccgaacca gaaatcgcgc ccgcagctcc tgcatcaatc gctgctttta cctgatccgg
      181 ggcggaaata ccaaatccct gcaatggagg tgcagcgttg tactctttca gcttcgcaac
      241 cagatgattg aggggtaacg cggcgcggtt ttctgcgccg gtcacgcctg ctcgtgacag
      301 caaataggtg taaccacgac cgtaagaggc tatctggcgc agcaggtcgt catcggcatt
      361 tggcgggcag atgaagatag gtgcgacatt atgacgcaac gcggcctggc ggaagggcgc
      421 ggactcttca actggcacat cggcaaccag caccgaatcg acgccgactt tttcgcactg
      481 ggcataaaac tcatcaatgc ctttgttaaa caccagattg gcatacatca acaggccaat
      541 gggaatggtc gggtgtttct ggcgaatcag tgccagcatt tcaaaacatt gtgccggagt
      601 cacacctgcc gcaaaggcgc gcagagtggc gttttgaatc gtcgggccat ccgccagtgg
      661 gtcggagaag gggataccta actccagcgc gtcagcaccg gcttcaatta gcgtatcgat
      721 aattttcaat gactgctcaa tgcccggatc accgagcgtg acgaaaggaa cgaatgcgcc
      781 ttctttgcgc tccttcaact gggcaaacag agattcgtag cgttccatca gatttcccct
      841 cgtgctttca aaatatcgtg aacggtgaag atgtctttat cgccgcgacc ggaaaggtta
      901 accaccagta gctgctcttt atccgggttt tcgcgcatca ttttcaacgc atgggccagg
      961 gcgtgggagg attccagcgc cgggatgatc ccttcgtgca ggcacagcgt tttgaaggct
     1021 tcaagggctt catcatcggt aatagacacg taatcagcgc gtccagtgct gttaagatac
     1081 gcgtgttgtg ggccgacaga cgggaaatcc agtccggcgg agatggagta agattcttca
     1141 atctgcccgt cttcggtttg catcatcggc gctttcatac cgaaatagat acccacgcga
     1201 ccatgtttta gcggtgctcc gtgctcgcca gtttcgatac cgtgaccacc tggctccaca
     1261 ccaatcaggc cgacgttggt ttcattgatg aaatcagcaa acatgccgat ggcattcgaa
     1321 ccgccgccaa cacaggcgat aacggcatcc ggcaggcgac cttctctttc cagaatctgc
     1381 gctttggttt cttcgccaat catccgctga aactcacgca caatggtcgg ataaggatgc
     1441 gggccagctg cggtgcccag catatagtgc gcggtttcgt aactaccgga ccagtcgcgc
     1501 agcgcctcgt tacaggcatc tttcagcgtc gcggaaccgc tatgcaccgg gatcacttcc
     1561 gcacccatta agcgcatacg aaaaacgtta ggcgactggc gttcaacgtc tttggcaccc
     1621 atataaatac ggcatttcag gccgagcagg gcgctggcaa gggccgacgc cacgccatgc
     1681 tgaccggcac cggtttcggc gatgatttcg gttttaccca tccgcttcgc cagcaacgcc
     1741 tgccccagca cctggttagt tttatgcgcg ccgccgtgca gcaaatcttc acgcttgaga
     1801 tacagcgtgg tgttcgtccc ggctgtaatg ttctggcatt tggtcagcgc ggttggacgc
     1861 ccggcatagt ttttcagcag gtcgttgaac tgagcctgaa attcaggatc tttttgcgca
     1921 ctgacaaaag cttcttccag ctggcgcaga gcaggcatca ggatttgtgg cacgtacatg
     1981 ccgccaaact caccaaaata ggggttaagt aatgttgtca ttgttccttt ccttaatatg
     2041 cgcgcagcgt ctggaaaacc gaggccaaaa gacgtgcgtc tttgatgccc ggttgcgact
     2101 ctacagcaga attaaaatca agtccggcgc agccggtttg tgccgcttcc acgcagttat
     2161 ctgcgcctaa gcccccagcc agcagaacgt tgccaagcga ttgaccattt aatagtgacc
     2221 agtcaaaacg ttgcccgctt ccaccctggc cgttgtctaa aacatattta tcaacgtgct
     2281 gaaactcgcg ggcgggcagg gtttcaccga cgcttaatgc tttccagatg gcaacatgtg
     2341 ctggcagagc ttcacgcagc gtatcgatat acagctgttc ttcattacca tgcagttgca
     2401 ctgccgccag cgataacacc ttagctttgt ccaccacatc ggcaatatcg tgattgcgga
     2461 acacgccaac atactgcaac ggtgccgcag ccatcacttc ctgcgcctgt tcaacgttga
     2521 cgcaacgcgg tgatgtcgca acaaaaatca acccaccgta aatcgcgccc gcgtcataag
     2581 ctgctttagc atcttgccca cgcgtcaggc cacatacttt attctcaccc agcaacaccc
     2641 ggcgcacggc ggcgtgcaaa tcgtcatggg ccatcaacgc cgaaccaatc agaaaaccgt
     2701 tagcgaagtg gcttaactcg cgcacctgag cgtaagtatt gatgccggat tcgctgatta
     2761 ccgtcacgtt gtgccccagt ttcggcgcaa gctcgcgggt acggttgaga tcaatcgaca
     2821 aatcacgcag atcgcggttg ttgatgccaa cgacctttgc ccccaatgca atggcacgct
     2881 ccagttcctc ttcattactg acttcggtca gcacacccat ctccagactg tgggcgacgg
     2941 cggcaagctg gcgatattgt tcgtcatcca gtactgaaag cattaataag caggcatcag
     3001 cctggtaata gcgcgccaga tagatctggt aaggatcgat aatgaagtct ttacataaaa
     3061 tcggctgcgg ggcgatttgg ctgacgatgg ggaggaaatc aaagctcccc tgaaaatatt
     3121 tctcatcagt cagcaccgaa attgccgaag cgtaatgttt ataaatggcg gcaatgcgtg
     3181 ctggatcgaa atcatcacgg atcacgcctt ttgacggcga cgctttcttg cactccagaa
     3241 taaacgccgt gcgcgcaccc tgtagcgcat cataaaaatg tcgcgtgctc ggctgaacct
     3301 cattctgaaa actggccagc ggttgctgct gtttgcgggt ttctacccaa atcgccttgt
     3361 ctgcgacgat tttcgctaaa acggtttgca tcatttaccc tcgtgccgcc agtgcggtaa
     3421 ctctgtcgta agcggaacca ctgcgcagta cctcaagaac ggtttgcgca ttggcttgca
     3481 gatcttcatg gccatgcagg cgcattaaca tggcgacgtt cgcagcgacg gctgcttcat
     3541 gggcggcgtc gcctttacct tgtaacaagc gtgttaaaat gtcacggttt tcttccggtg
     3601 ttccgcctgc cagttgctcc tggtggtagg gagtcaggcc aaaatcttca gcggtcaatt
     3661 gatagctctt aatttcgccg tcatgcagct cggcaacgat tgtcggcgcg tgtaatgaaa
     3721 cttcatccat cccgccgctg tgcaccaccg ccgcgcgttg ataccccagc acgcgcaagg
     3781 tttcggcaat cggcagcacc agttccggac tataaacacc aattaacgcc agcggcggat
     3841 gcgccgggtt aatcaatggc cccagcacat tgaacagggt gcgggttttc agttgctggc
     3901 gaaccggcat cgcgtggcgg aatccggtgt gatacttcgg cgcaaagagg aaacatacac
     3961 ctaactcatc cagcgcctgg cgcgatttat cggcgttcat atcaagatta ataccgaacg
     4021 ccgccagcag atcggacgaa ccagatttac tggagacgct acggttgccg tgtttcgcca
     4081 ctttcagccc acaggccgcg gcgacaaacg cactggcggt agaaatattg atactgttgc
     4141 tgccgtcacc gccagtaccg acgatatcag caaacagata atccgggcgc gggaacggcg
     4201 cggcgttttc cagtagcgcg gttgctgctc cggcgatctc gttcgggtgc tcaccgcgaa
     4261 ttttcatgct caccagcgcc gccgccagtt gttccggctt cagctcgcca cgcaccaccg
     4321 ctgaaaacag ctggtggctt tcttgttggc taagcgtctg cgcctgatac agtttttcca
     4381 gaatcggttg cagcgtgttg gctggctcta gtttctgctg cgcccaggcc agcgtttgtt
     4441 ccagcaggcg agcgccctgg gtggtgagaa tggattccgg atggaactgg aatccacaaa
     4501 cgcgatccgc atcgtgacgt actgccatca ccatgccatt aaaatgggcg ttgatggtta
     4561 aaccggccgg aatgttactg ccaaccagcg agtgataacg cgccaccggc agcgggtttg
     4621 ttaatccggc aaacatcgcc tgaccgtcat gttcaatgct cgacgcttta ccgtgaagaa
     4681 tttcgcccgc ctgaccgaca tagcccccgt aagcttcgac aattgcctga tgtccgaggc
     4741 aaatgccaat aatgggcagc ttgccacgca agcgggtgag gagttccggc atacaaccgg
     4801 cttcgctcgg cacaccgggg ccaggagaaa gcatcagtac cggattgctc atcgtcgcca
     4861 ggcgttcaat taaggtctgc gccggaatat ggttgcggta aatcaccacg ttatgaccat
     4921 tgctgcgcaa ctgatctgcc aggttgtacg taaaagagtc gatattatcg agcagcagaa
     4981 tgtcagccat cagaaagtct cctgtgcatg atgcgcggtg gcaatagcgc gcagtacagc
     5041 gcgggctttg ttacgggttt cgtcggcttc cgactgcgga acagaatcaa ggactacacc
     5101 agcacccgct tgcacggtgg cgataccgtt ttccaccagc gccgagcgga tcacaatgca
     5161 ggtgtcgaga tcgccatgcg cggtgaaata acctaccgcg ccgccgtagc tgccgcggcg
     5221 acgaccttcc gcctcggcaa ttaactgcat agcgcgtact ttcggcgcac cgcttaacgt
     5281 ccccatattc atacaggcgc gataagcgtg cagggcgtca agatcgtgac gcagttcgcc
     5341 gactacgcga gagacgaggt gcatcacata ggaataacgg tcaactttgg tgagatcggc
     5401 gacgtagcgg ctgccggggg tgcaaatgcg tgccagatca ttacgggcga gatcaaccag
     5461 catcagatgt tcagacagct ctttatgatc ggtacgcatt tccagttcaa tacggctgtc
     5521 gagatctctg tccagtgaac catcggcgcg acgaccgcgt gggcgtgttc cggcaatcgg
     5581 gtagatctca atctggcggc tggtggcatc atacttgagc gagctttccg gcgacgcgcc
     5641 aaatagggtg aaatcattat cctgcataaa aaacatgtac gggctgggat tactcttttt
     5701 cagcacgtaa taggccgcca gcggtgacgg gcagggcaga gagaaacggc gagatggcac
     5761 cacctggaaa atttctccag cgcgaatcgc tttttgcaac aaacgcacta cgccaccgaa
     5821 ctcttcatcg ctctgattac attcacaacg catatgcggc acggaaacca ctggcagcgg
     5881 cggcgcggct tcggtcagtt gctgacgtag ttcgttcagg cgagcagtga gacgttgttt
     5941 ttcttcttca ttcggagcaa acaggctggc ctgaatacgg gtgctttttt tctgatggtc
     6001 aatcaccatc agcgtttcag cgagataaaa acagaaatca gggcagttat tttccgctga
     6061 cagttgcggt aaatcttcaa atcccgccac aaggtcataa gagaacaggc cgccgaagaa
     6121 catggcttct cgttcttcct tcggtacatt caacagattc tgcaataaac ggaaagcgtc
     6181 aaaaaccgaa agggagcata agcgggcgtc ttcatccagc agtggactga caggggggaa
     6241 gcgcagcaca cggcagtttg gtgattgttc actttccaca cccgcaggca gggcgttatc
     6301 cagtagtgcc agcagggctt cgccattgcc ggaaagtgcc tgaattgtga cggtgtcacc
     6361 taatgccgta atgcgcagcg cactgtctat cagcagcagg ctttttaaat catctttgct
     6421 gtcgatatct gcggattcca gcagcagcgt tgccggacga ttcccacaca actggtgaaa
     6481 aaacgcagtc gggttgtcgc gataagcgcc ttcgcaggtt agcagttcga gagtcggttt
     6541 ttgtgtttgc attgttattc tctaattttg ttcaaaaaaa agcccgctca ttaggcgggc
     6601 tgggtatctg attgctttac gcatggtgaa tacactgccc gtttcaggaa gtgcgccacc
     6661 aacctttcag tacgaaaatt gctttcattg tcgataccct ttttacgtga actcgcgtac
     6721 tagttaacta gttcgatgat taattgtcaa cagctcattt cagaatattt gccagaaccg
     6781 ttatgatgtc ggcgcaaaaa acattatcca gaacgggagt gcgccttgag cgacacgaat
     6841 tacgcagtga tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca
     6901 gaagcattgg tgcaccgtgc agtcgagatg cgcgtcggca ccctggcgat caccgaccat
     6961 gacaccacag ctgcaatcgc gccagcaaga gaggaaattt cacgttcggg actggcgctg
     7021 aatcttattc ccggcgtgga aatttccacg gtctgggaaa atcatgaaat tcatattgtc
     7081 gggctgaata ttgatattac tcatccgcta atgtgtgagt tcctcgcgca gcagacagaa
     7141 cggcgcaatc agcgggcgca gctgattgcc gaaagacttg aaaaagcgca aatacctggg
     7201 gcgctggaag gcgcacaacg actggcgcag ggcggcgcag tgacgcgcgg tcattttgcg
     7261 cgtttccttg ttgagtgcgg caaagccagt tcaatggcgg atgtctttaa aaagtatctg
     7321 gcgcgcggga aaaccggata cgttccgcca cagtggtgta caatagaaca agctattgat
     7381 gtcattcatc attctggcgg taaggcggtg ctagctcatc ctgggcggta caatcttagt
     7441 gctaaatggc tgaaaagatt ggttgcgcat tttgccgaac accacggtga cgcgatggaa
     7501 gtcgcgcagt gccagcaatc gcccaatgaa cgtacccagc tggcggccct tgcgcgtcag
     7561 catcatttat gggcatcaca aggatctgat tttcatcagc catgcccgtg gatcgaactg
     7621 ggtcgtaaac tctggctgcc cgcaggcgtt gaaggcgtct ggcagctatg ggaacagccg
     7681 cagaacacca cagagaggga attatgagcc agttttttta tattcatcct gataacccac
     7741 agcaacgtct gatcaaccag gcggtggaga tcgtgcgtaa aggcggggtg attgtttatc
     7801 caactgattc cggctatgcg ctcggctgta aaattgaaga taaaaacgcg atggagcgta
     7861 tttgtcgtat tcgccagctg ccggacggtc acaactttac cctgatgtgt cgcgatcttt
     7921 ctgaactctc gacctattca tttgttgata acgttgcgtt tcgtttaatg aaaaacaaca
     7981 cgccgggcaa ctataccttc atcctgaagg ggacgaagga agtgccacgc cgtctgttgc
     8041 aggaaaaacg caaaaccatc ggtatgcgtg tgccttctaa cccgattgcg caggcgttgc
     8101 ttgaagcact gggcgaaccg atgctttcca cttcgctaat gctgccaggc agcgaattta
     8161 ccgaatccga tcccgaagaa attaaagacc gtctggaaaa acaggtagat ttgattattc
     8221 acggcggcta tctcgggcag aaaccgacaa cggttattga tcttaccgat gatacgccgg
     8281 tcgtggtgcg tgaaggcgta ggtgatgtga agcctttctt ataacaaatt gcgttccacg
     8341 gatggaagac tatggcaggg aagtttcgct gcattttgct gttgatagca gggctgtttg
     8401 tatcatctct aagttatgca gaaaacacgg agatcccttc ttatgaagaa gggatttcgc
     8461 tctttgatgt tgaagccact ctgcaaccgg atggggtgct cgacatcaaa gaaaatattc
     8521 attttcaggc gcgaaatcag cagattaaac acggatttta tcgtgattta ccacgactct
     8581 ggatgcagcc tgatggggac gctgcactgc tgaactatca tattgttggc gtcacccgtg
     8641 atggtattcc tgaaccctgg catcttgact ggcatatcgg gttaatgatt attgtcgtgg
     8701 gcgataaaca acgtttcttg cctcaaggcg actatcatta tcaaattcat tatcaggtta
     8761 aaaatgcttt cctgcgtgag ggggattctg atctgctaat ctggaacgtg accggtaacc
     8821 actggccgtt tgaaatttat aagacccgtt tttctctcca gttctctaat attgcgggta
     8881 atccatttag cgaaatcgat ctctttaccg gagaagaggg cgacacatat cgtaatggcc
     8941 gcatccttga ggacggaaga attgaatccc gcgatccgtt ttatcgtgaa gatttcacgg
     9001 tcctctaccg ctggcctcac gctttactta gcaatgcctc ggctccgcaa acgacgaata
     9061 ttttcagcca tcttctttta ccctccacgt catcgttgtt aatttggttt ccgtgtctgt
     9121 tcctggtttg tggatggtta tatctctgga agcgcaggcc gcaatttacg ccggtagatg
     9181 tgattgaaac cgatgtcatt ccgccagatt acacgcccgg catgttacgt ctcgatgcga
     9241 agctggttta cgacgataaa gggttttgtg ccgatatcgt aaatctgatt gtgaaaggaa
     9301 aaattcatct ggaagatcag tatgataaga accagcaaat cctgattcgt gttaatgaag
     9361 gcgcgaccag aaataatgag gtattactgc ccgcagagca gttattactg gaagcgttat
     9421 ttcgtaaatg cgataaggtc gttcttacag ggagacgcaa cagagtctta cgcagggcat
     9481 ttttacggat gcagaaattt tatctgccgc gtaaaaagtc ttcgttttac cggcctgata
     9541 cgtttttgca atggggtgga ctggcaatat tggcggtcat tctttacggt aacttgagtc
     9601 ctgtaggctg ggcaggaatg agtctggtgg gcgatatgtt tattatgatc tgctggatta
     9661 ttcctttttt attttgttcc cttgagcttt tgtttgcccg cgatgatgac aagccttgcg
     9721 ttaatcgtgt aatcatcact ttgtttttac cactgatttg ttcaggcgtg gccttctatt
     9781 ctctctacat caatgtcgga gatgtattct tttactggta tatgccagcg ggttatttta
     9841 ccgctgtttt cctgaccggt tatctcactg gcatggggta tatttttctg ccaaagttta
     9901 cccaaactgg acagcaacgt tatgcccacg gtgaagctat cgttaactat cttgcgcgta
     9961 aagaggcggc aacacacagt gggcggcgtc ggaaagggga aacacggaaa ctggattatg
    10021 cgttgctagg ttgggcggtc tcggcaaacc ttggcagaga atgggcagca cgtatcaccc
    10081 catcactcac agcggctgtt cgcgccccgg aaattgcccg tagcggcgtt ttattctcat
    10141 tacagacgca cctaagttgc ggggcctata ccagtttgtt ggggcgaagt tattccggtg
    10201 gtggtgctgg cggcggcgcg ggtggcggag gcggtggtgg ctggtaatta agctgatgtt
    10261 aatcgaaaca agctttaacc ataaagatag tgtagtatgt tgcgcctcaa agccaggcca
    10321 gttaaaacgt cgagtcgttt acttaaggcc tgaagagttc aaacagactt tactatcagt
    10381 gagtcagatg taagtcctga ctcattgttg cacaaaagca tatcagacgc ctgggaaggc
    10441 gacaccaaaa ggaagctcta tgagcgaaaa gctacagaaa gtgctggcgc gtgccggcca
    10501 cggttctcgc cgtgaaatcg aatctattat tgaagccggt cgtgtgagtg ttgatggcaa
    10561 aattgctaaa ctcggcgatc gcgttgaagt tacccctgga ttgaaaatcc gtatcgatgg
    10621 tcacctgatt tcggtacgtg agtccgctga acaaatttgt cgcgttctgg cctattacaa
    10681 accggaaggt gagttgtgta cccgtaacga tccggaaggg cgtccaacgg tgtttgaccg
    10741 tctgccaaag ctgcgtggcg cacgctggat tgccgtaggt cgtctggacg ttaacacctg
    10801 tggtctgctg ctgttcacca ccgacggtga actggcaaac cgtttaatgc atccaagccg
    10861 tgaagttgaa cgtgaatatg ccgtgcgtgt atttggtcag gttgacgacg cgaaactgcg
    10921 tgatttgagt cgtggcgtgc agctggaaga tggtccggca gcttttaaaa ccatcaagtt
    10981 cagcggcggc gaagggatca accagtggta caacgtgact ctgaccgaag gccgtaaccg
    11041 tgaagttcgt cgtctgtggg aagcggttgg tgttcaggtg agccgcctga ttcgtgttcg
    11101 ttacggtgat atcccactgc caaaaggtct gccacgcggt ggctggacgg agctggacct
    11161 cgcccagact aactatctgc gcgaactggt ggagctaccg ccagaaacca gctctaaagt
    11221 cgctgtagaa aaagaccgtc gtcgcatgaa ggcgaatcag attcgtcgtg cggtgaaacg
    11281 tcacagtcag gtgagcggcg gtcgtcgttc tggcgggcgt aataacggtt aatcaaagta
    11341 tgccggacgt catatccggc atttttacag attaataatc aatccctatt tgcgccttaa
    11401 caccggcatc aaacgcatgt ttgatggggc gcaactcact caccgtatcc gccagttcaa
    11461 gaatatcccg atgacagcct cgacccgtga taatcaccgt ctgttgatgt ggacgttcat
    11521 ttaacgcctg caccacttct tccagtggca aatagtcata cgccaccata taagtcagct
    11581 catcaagcaa aaccatatcc agtgaggaat cagcaagcat ccgctttgca tgttgccaga
    11641 cttcacggca ggccgcggta tcagattcgc gattttgtgt atcccaggta aagcccgttg
    11701 ccatcacctg aaactcaacg ccatgtggct ccagcagatt gcgttcgcca ttaggccagg
    11761 tgcctttaat aaactgcacg acgcccactt tttttccgtg accaactgcg cgtgttgccg
    11821 taccaaatgc cgcggtagtt ttgccttttc cattgccggt aaagacgatg ataataccgc
    11881 gttcatcctg ggcctgggcc acacgagcat ctactttttc tttcactcgc tgctgacgct
    11941 gttggtagcg ttcatcactc attgggaaat tcctggttta cggcccggtt gggcgtcaaa
    12001 ggtcatgccg gttttacggc ggctgtcatc gcccatcagc cagaggtaga gcggcatgat
    12061 atcagcgggt gttttaagtt tctgtggatc ttcggtcggg aaggcgctgg cacgcattgc
    12121 ggtgcgcgta ccgcctgggt taatgcagtt gacacgcagg cgctgctgat attcatcggc
    12181 cagtacctgc atcatccctt cggtggcaaa tttcgacgct gcatatgcgc cccagttggc
    12241 tcgtccctga cgtccaacgc ttgatgaagt aaagaccagt gaaccggcgt ccgatttgag
    12301 taataaagga agcagtgcct gggtgagcat aaaggtggca ttaacgttga cctgtatgac
    12361 gtcctgccag acctgtggat tttgttcgct cattgggcaa acatcgccga gcaatccggc
    12421 attatgcaaa acaccatcca gacgcggata attaacggta atgcgctgtg ccagttgttg
    12481 gcaatcttcg gacgtgcagg tcagcaaatc gagaataaac cactgtggct gacgtccagt
    12541 ttcttcgttt atgtggctgg ctacctgacg taatttttct tcattacggc ccaacagaat
    12601 cactgtcgca ccatagcgtg catacgtcat cgcggcttca cggccaatcc catcgctggc
    12661 tcccgtcacc aggataatgc gatcattgag taaatcttgt tttggctggt aatgcatggc
    12721 tactcctcaa cgacgttgtc tgtcgcgctg ccagtgtact ttatgacttt ggggctttat
    12781 gcctgaaaca gaagggcatt tcaatcagcc tggggcgata acaccgcaaa atggatactt
    12841 tgtcatactt tcgctgcaat aacatctctg cgagacggct taacatgcct gttgtaaact
    12901 gtgagccaaa gcgttgttta accaaggtgg ggactcgtgg aattgttgtc tgaatatggt
    12961 ttgtttttgg cgaaaatcgt taccgttgtg ctagcgattg cggcgattgc cgccattatt
    13021 gtcaatgttg ctcaacgtaa taaacgccag cgtggcgagt tacgggtcaa caatctcagc
    13081 gaacagtata aggagatgaa agaagaactg gccgcggcgc tgatggactc acatcagcaa
    13141 aaacagtggc acaaagcgca gaagaaaaag cacaagcaag aagcgaaagc agcaaaagcg
    13201 aaagccaaac tgggcgaggt ggcaactgac agtaaacctc gcgtctgggt gctggatttt
    13261 aaaggcagca tggacgccca tgaagtgaac tcgctacgtg aagagataac ggcggtactc
    13321 gcagcattca aaccgcagga tcaggttgtg ctccgtctgg aaagccctgg tggcatggtg
    13381 catggttacg ggttggcggc ttcgcagctg cagcgtctgc gtgataaaaa cattccttta
    13441 actgttacgg tagacaaagt cgctgccagc ggcggttaca tgatggcctg tgtggcggac
    13501 aaaattgttt ccgcaccgtt tgctattgtg ggttccattg gggtggtggc gcaaatgccc
    13561 aactttaacc gcttcctgaa aagcaaagat attgatatcg aactgcacac tgccgggcag
    13621 tataagcgta ctctgacgtt gctgggtgaa aataccgaag aagggcggga gaaattccgc
    13681 gaagagttga acgaaacgca tcagttgttt aaagattttg tgaagcgtat gcgtccgtct
    13741 ctggatattg aacaggtggc aacgggtgaa cactggtacg gacaacaggc ggtagagaaa
    13801 ggcctggttg atgaaatcaa caccagtgat gaagttattc ttagcctgat ggaaggccgt
    13861 gaagtggtta atgtacgcta tatgcagcgt aaacgactca ttgaccgatt caccggcagc
    13921 gcggcagaga gcgccgatcg attgttgcta cgctggtggc agcggggtca aaagccattg
    13981 atgtaaaaga caaacgcgag gctaagacct cgcgttttgc gttaatcaac cagatgatat
    14041 ttttctgaaa gcacatgggc caggtgtttg aacatattaa acaccgcggt gcttttggct
    14101 gttggcaatc cttgttcatc taaaaagtag tcgccggtaa ataccagcac gccattacgc
    14161 tgcgtgacgc cggtggcttc aatccctgcg agcgtatcct catgctcacg aatgattttg
    14221 ttggcctctt tcaacagcgt ttcgcggtcg atgggttgtg tctctttgtt cattatttac
    14281 tccttaaaca aggacattag tctacgccag gcatggcttg cagacaaata taccacgctg
    14341 gtggcaagag cgccttactg gcaactttgg attttgcatg ctaataaagt tgcgtatcgg
    14401 attttatcag gtacagtgtg acgctttcgt caatctggca atagatttgc ttgacattcg
    14461 accaaaattc cgtcgtgcta tagcgcctgt aggccaagac ctgttaactc agtcacctga
    14521 attttcgtga acagagtcac gacaaggggt tgatatccgc agagaacgag tccatatcgg
    14581 taactcgttg ccagtggaag gtttatcaac gtgcgacgca ttcctggaag aatcaaatta
    14641 ggtaaggtga atatgggtaa agctcttgtc atcgttgagt ccccggcaaa agccaaaacg
    14701 atcaacaagt atctgggtag tgactacgtg gtgaaatcca gcgtcggtca catccgcgat
    14761 ttgccgacca gtggctcagc tgccaaaaag agtgccgact ctacctccac caagacggct
    14821 aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc gtatgggggt tgacccgtgg
    14881 cacaattggg aggcgcacta tgaagtgttg cctggtaaag agaaggtcgt ctctgaactg
    14941 aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa ccgaccttga ccgcgaaggg
    15001 gaagccattg catggcacct gcgggaagtg attggtggtg atgatgcgcg ctatagccga
    15061 gtggtgttta acgaaattac taaaaacgcg atccgccagg catttaacaa accgggtgag
    15121 ctgaatattg atcgtgttaa tgcccagcag gcgcgtcgct ttatggaccg cgtggttggg
    15181 tatatggttt cgccgctgct atggaaaaag atcgctcgtg gtctgtctgc cggtcgtgtg
    15241 cagtcggtgg cggttcgcct ggtggtcgag cgtgagcgtg aaattaaagc gttcgtgccg
    15301 gaagagttct gggaagtcga tgccagcacg accacgccat ctggtgaagc gttggcgtta
    15361 caggtgactc atcagaacga caaaccgttc cgtccggtca acaaagaaca aactcaggct
    15421 gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg aacgtgaaga caaaccgaca
    15481 accagtaaac ctggcgctcc ttttattacc tctacgctgc aacaagctgc cagcacccgt
    15541 cttggatttg gcgtgaaaaa aaccatgatg atggcgcagc gtttgtatga agcaggctat
    15601 atcacttaca tgcgtaccga ctccactaac ctgagccagg acgcggtaaa tatggttcgc
    15661 ggttatatca gcgataattt tggtaagaaa tatctgccgg aaagtccgaa tcagtacgcc
    15721 agcaaagaaa actcacagga agcgcacgaa gcgattcgtc cttctgacgt caatgtgatg
    15781 gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac tgtaccagtt aatctggcgt
    15841 cagttcgtag cctgccagat gaccccagcg aaatatgact ccacgacgct gaccgttggt
    15901 gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc gttttgatgg ctggacaaaa
    15961 gtgatgcctg cgttgcgtaa aggcgatgaa gatcgcatct taccagcagt taataaaggc
    16021 gatgctctga cgctcgttga acttacacca gcccagcact ttaccaagcc gccagcccgt
    16081 ttcagtgaag catcgctggt taaagagctg gaaaaacgtg gtatcggtcg tccgtctacc
    16141 tatgcgtcga tcatttcgac cattcaggat cgtggctacg tgcgagtaga aaatcgtcgt
    16201 ttctatgcgg aaaaaatggg cgaaatcgtc accgatcgcc ttgaagaaaa tttccgcgag
    16261 ttaatgaact acgactttac cgcgcagatg gaaaacagcc tcgaccaggt ggcaaatcac
    16321 gaagcagagt ggaaagctgt actggatcac ttcttctcgg atttcaccca gcagttagat
    16381 aaagctgaaa aagatccgga agagggtggt atgcgcccga accagatggt tctgaccagc
    16441 attgactgcc cgacttgtgg tcgcaaaatg gggattcgca cagcgagcac cggggtattc
    16501 cttggctgtt ctggctatgc gctgccgccg aaagagcgtt gcaaaaccac cattaacctg
    16561 gtgccggaaa acgaagtgct gaacgtgctg gaaggcgaag atgctgaaac caacgcgctg
    16621 cgcgcaaaac gtcgttgccc gaaatgcggc acggcgatgg acagctatct catcgatccg
    16681 aaacgtaagt tgcatgtctg tggtaataac ccaacctgcg acggttacga gatcgaagag
    16741 ggcgaattcc gcattaaagg ttatgacggc ccgatcgttg agtgtgaaaa atgtggctct
    16801 gaaatgcacc tgaaaatggg gcgttttggt aaatacatgg cctgcaccaa cgaagagtgt
    16861 aaaaacacac gtaagatttt acgtaacggc gaagtggcac caccgaaaga agatccggtt
    16921 ccgctgccag aactaccttg cgaaaaatcg gatgcttatt ttgtgttgcg tgacggcgct
    16981 gcgggtgttt tcctggctgc caacactttc ccgaaatcgc gtgaaacgcg tgcgccgctg
    17041 gtggaagagc tgtatcgctt ccgcgaccgt ctgccggaaa aactgcgtta tctggccgat
    17101 gcgccgcagc aggatccgga aggtaataag accatggttc gctttagccg taaaaccaaa
    17161 cagcaatatg tctcttcgga aaaagacgga aaggcgactg gctggtcagc attttatgtt
    17221 gatggcaaat gggttgaagg gaaaaaataa cctttaattc tgtcagtttt ttataaacaa
    17281 agggtcgcga aagcggccct tttttattgc atattatttt ttcttcacac ctatacacta
    17341 aggctataaa tgatatagtg gttatagtta gcaccttttt tattattaaa tcgtattagt
    17401 cacgccgcct ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg atggtttaac
    17461 atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct gaatgtctca
    17521 tcaacagcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt cagaatgctg
    17581 gaagacgagc taggcattca aattttttcc cgaagcggca agcacctgac gcaggtaacg
    17641 ccagcagggc aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt cgatgccata
    17701 aaatcggttg ccggagagca cacctggccg gataaaggct cactgtatat cgccaccacg
    17761 catacccagg cacgctacgc attaccaaac gtcatcaaag gctttattga gcgttatccc
    17821 cgcgtttctt tgcatatgca ccagggctcg ccgacacaaa ttgctgatgc cgtctctaaa
    17881 ggcaatgccg atttcgctat cgccacagaa gcgctgcatc tgtatgaaga tttagtgatg
    17941 ttaccgtgct accactggaa tcgggctatt gtagtcactc cggatcaccc gctggcaggc
    18001 aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata taccttcggc
    18061 tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac gccgcgtatc
    18121 gtcttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg gctgggggta
    18181 ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt gcgtgttgat
    18241 gctcacgata tcttcagcca cagtacaacc aaaattggtt ttcgccgtag tacttttttg
    18301 cgcagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg tgatgtcgtt
    18361 gatgcggctg tcgcattgcg ctctaatgaa gaaattgagg tcatgtttaa agatataaaa
    18421 ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg aaggtgccgg
    18481 aatatattct cttttgttac ttatttgtat accgtgcaca atatttttat ccctcatatt
    18541 tatagggtaa attacctgta aagtagtgtc taattacccg cctcgcaaat tccagttttc
    18601 aaatatctat ttcaggtcaa aagattgaat tattcactgg agacgattcg taaattcgct
    18661 ggatttttga ctaaagtttc tttaggattt gtctcatcgt atgattaatt acaccaatcg
    18721 ttcaacgtct tgctgataag tgatggcgat tatatgaggt tagcaatgcc ttctggaaat
    18781 caggaacccc gccgcgatcc tgaattaaaa cgtaaagcct ggctggcggt ttttcttggt
    18841 tctgcacttt tctgggtggt tgtcgcactg ctgatttgga aagtgtgggg ataactatgg
    18901 ttggtcagga gcaactggag tcgtcaccat tatgccagga tagtgacaat gaaccagaaa
    18961 cgaaacggga atgttccgtc gttattccag acgactggca actaacatcg cagcagcaag
    19021 cctttataga actgtttgct gaagatgatc agccgaaaca ataattatca tcattcttat
    19081 tacccatttt taatgaatta aagggctttt aatacaccgc agcaataaca ggttgagtta
    19141 tctcaacaca aagtaataac cgttaagggt gtagcctatg atcaacacaa atatgaaata
    19201 ttggtcctgg atgggcgcgt tttctctgtc gatgctcttc tgggccgaac tcctctggat
    19261 cattactcac tgatccttga tcccgctgcg gcggggtttt catttgcttt gccacaagct
    19321 ttctcctctt ttatcaattt gggttgttat caaatcgtta cgcgatgttt gtgttatctt
    19381 taatattcac cctgaagaga atcagggctt cgcaaccctg tcattaagga ggagctatgt
    19441 cgtcaaccct acgagaagcc agtaaggaca cgttgcaggc caaagataaa acttaccact
    19501 actacagcct gccgcttgct gccaaatcac tgggcgatat cacccgtttg cctaagtcac
    19561 tcaaagtttt gctcgaaaac ttgctgcgct ggcaggatgg taactcggtt accgaagagg
    19621 atatccacgc gctggcggga tggctgaaaa atgcccatgc tgaccgtgaa attgcctacc
    19681 gcccggcaag ggtgctgatg caggacttta ccggcgtacc tgccgttgtt gatctggcgg
    19741 caatgcgcga agcggttaaa cgcctcggcg gcgatactgc aaaggttaat ccgctctcac
    19801 cggtcgacct ggtcattgac cactcggtga ccgtcgatcg ttttggtgat gatgaggcat
    19861 ttgaagaaaa cgtacgcctg gaaatggagc gcaaccacga acgttatgtg ttcctgaaat
    19921 ggggaaagca ggcgttcagt cggtttagcg tcgtgccgcc aggcacaggc atttgccatc
    19981 aggttaacct cgaatatctc ggcaaagcag tgtggagtga attgcaggac ggtgaatgga
    20041 ttgcttatcc ggatacactc gttggtactg actcgcacac caccatgatc aacggccttg
    20101 gcgtgctggg gtggggcgtt ggtgggatcg aagcagaagc cgcaatgtta ggccagccgg
    20161 tttctatgct tatcccggac gtagtgggct ttaaacttac cggaaaatta cgtgaaggta
    20221 ttaccgccac agacctggtt ctcaccgtta cccaaatgct gcgcaaacat ggcgtggtgg
    20281 ggaaattcgt cgaattttat ggtgatggtc tggattcact accgttggcg gatcgcgcca
    20341 ccattgccaa tatgtcgcca gaatatggtg ccacctgtgg cttcttccca atcgatgctg
    20401 taaccctcga ttacatgcgt ttaagcgggc gcagcgaaga tcaggtcgag ttggtcgaaa
    20461 aatatgccaa agcgcagggc atgtggcgta acccgggcga tgaaccgatt tttaccagta
    20521 cgttagaact ggatatgaat gacgttgaag cgagcctggc agggccaaaa cgcccacagg
    20581 atcgcgttgc actgcccgat gtaccaaaag catttgccgc cagtaacgaa ctggaagtga
    20641 atgccacgca taaagatcgc cagccggtcg attatgttat gaacggacat cagtatcagt
    20701 tacctgatgg cgctgtggtc attgctgcga taacctcgtg caccaacacc tctaacccaa
    20761 gtgtgctgat ggccgcaggc ttgctggcga aaaaagccgt aactatgggc ctcaagcggc
    20821 aaccgtgggt caaagcgtcg ctggcaccgg gttcgaaagt tgtttctgac tatctggcaa
    20881 aagcgaaact gacaccgttt ctcgacgaac tggggtttaa ccttgtggga tacggttgta
    20941 ctacctgtat tggtaactct gggccgctgc ccgatcctat cgaaacggca atcaaaaaag
    21001 gcgatttaac cgtcggtgca gtgctgtccg gcaaccgtaa ctttgaaggc cgtatccatc
    21061 cgctggttaa aaccaactgg ctggcctcgc cgccgctggt ggttgcctat gcgctggcgg
    21121 gaaatatgaa tatcaacctg gcttctgagc ctattggtca tgatcgtaaa ggcgatccgg
    21181 tttatctgaa agatatctgg ccatcggcac aagaaattgc ccgtgcggta gaacaagtct
    21241 ccacagaaat gttccgcaaa gagtacgcag aagtttttga aggcacagca gagtggaagg
    21301 gaattaacgt cacacgatcc gatacctacg gttggcagga ggactcaacc tatattcgct
    21361 tatcgccttt ctttgatgaa atgcaggcaa caccagcacc agtggaagat attcacggtg
    21421 cgcggatcct cgcaatgctg ggggattcag tcaccactga ccatatctct ccggcgggca
    21481 gtattaagcc cgacagccca gcgggtcgat atctacaagg tcggggtgtt gagcgaaaag
    21541 actttaactc ctacggttcg cggcgtggta accatgaagt gatgatgcgc ggcaccttcg
    21601 ccaatattcg catccgtaat gaaatggtgc ctggcgttga aggggggatg acgcggcatt
    21661 tacctgacag cgacgtagtc tctatttatg atgctgcgat gcgctataag caggaacaaa
    21721 cgccgctggc ggtgattgcc gggaaagagt atggatcagg ctcaagccgt gactgggcgg
    21781 caaaaggtcc gcgtctgctt ggaattcgtg tggtgattgc tgaatcgttt gaacgaattc
    21841 accgttcgaa tttaattggc atgggcatcc tgccgctgga atttccgcaa ggcgtaacgc
    21901 gtaaaacgtt agggctaacc ggggaagaga agattgatat tggcgatctg caaaacctac
    21961 aacccggcgc gacggttccg gtgacgctta cgcgcgcgga tggtagccag gaagtcgtac
    22021 cctgccgttg tcgtatcgac accgcgacgg agttgaccta ctaccagaac gacggcattt
    22081 tgcattatgt cattcgtaat atgttgaagt aacaagtatt tgcttgccgg ttattttgct
    22141 tccggcaagc aaatgaatta cacaatgcaa gagggttatt tgttcagcaa atggcccatt
    22201 ttctcggctt tggtatcgag ataatgttcg ttattggggt tacgacctac aatcaatggt
    22261 acgcgttcaa caatattaat ccctgcttcg gtcagaattt cgactttttt cgggttattg
    22321 gttaacaagc ggacttcatt gacgccaagg agtttgaaca tatcagcgca aagagtgaag
    22381 tcgcgctcat cagcggcgaa gcctaactgg tggttagcct ctacggtatc gtaaccttga
    22441 tcctgcagtg cgtaagcgcg gattttattc agcagaccaa tgttacgacc ttcctgacgg
    22501 tgatacagca aaataccacg gccttcctcg gcaatttgcg tcaatgccgc ttcgagctgg
    22561 aagccacaat cgcagcgcaa gctgaacagg gcgtcaccgg tcagacattc ggaatggacg
    22621 cgcgcaagta ccggggtatg cccggaaata tcgccataga ctagcgcgac atgatcgtgt
    22681 ccggttgcca gttcttcaaa tcccaccatc aggaaatcgc cccatggggt tggcagtttg
    22741 gcttctgcca cacgtttaag ctgcatgaaa ttctccagat aatgctggtt ctgtattggc
    22801 ttattttgcc ataacgagaa ggggttcacc taatcacgac gcgtcgatcg ttcacggaat
    22861 ggcacaaatc tgtcaatttt tcctggaact ggcattttca gttatgattg tgggacttat
    22921 caaaaaggag aggccatgcg ttcgattgcc cgacgtaccg cagtgggagc tgcactattg
    22981 cttgttatgc cagtagccgt atggatttct ggctggcgtt ggcaacctgg agaacaaagt
    23041 tggctactaa aagcggcttt ttgggttact gaaactgtca cccagccctg gggcgtcatt
    23101 acacatttga ttttattcgg ctggtttctc tggtgtctgc gttttcgcat taaggctgcc
    23161 tttgtattat ttgccattct ggcggccgca atccttgtgg gacaaggcgt taaatcctgg
    23221 atcaaagaca aagtccagga accacgacct tttgttatct ggctggaaaa aacacatcat
    23281 attccggttg atgagttcta cactttaaag cgagcagaac gcggaaatct agtgaaagaa
    23341 cagttggctg aagagaaaaa tatcccacaa tatttgcgtt cacactggca gaaagagacg
    23401 gggtttgcct ttccttccgg tcacacgatg tttgctgcca gttgggcact gctggccgtt
    23461 ggtttgctgt ggccgcgtcg gcgaacgtta accattgcta tcttgctggt ctgggcaacg
    23521 ggagtcatgg gaagccgcct gctgctcggg atgcattggc cacgcgatct ggtagtagct
    23581 acgttgattt cgtgggcgct ggtggcggtg gcaacgtggc ttgcgcaacg aatttgtggg
    23641 ccattaacac cacctgcgga agaaaatcgc gaaatagcgc aacgagaaca agaaagttaa
    23701 cgctggttga ttttccgaat ttggccctta aatcatcaac aatgcgcgtg gatgccattt
    23761 cgcagacggc gcgaaaatgg tactttaaag ggctattgcg gtaagttgac cataatttat
    23821 tcgctctaac cacataacgg gaagtaatgt gaaatattta ctcattttct tactggtgtt
    23881 agcgatcttc gtgatttcgg tcacgttggg tgcgcagaac gatcaacagg tgacgtttaa
    23941 ttatctgtta gcgcaagggg agtaccgtat ttccacattg ctggcggtat tgtttgctgc
    24001 ggggtttgct atcggttggt tgatttgtgg cctgttctgg ctgcgagttc gtgtttccct
    24061 ggcgcgcgct gaacgtaaaa taaagcgact ggaaaaccag ctttcacccg cgactgacgt
    24121 ggctgtagtg ccgcactcgt cagcggcgaa ggaataactt tctatgctgg agttgttgtt
    24181 tctgcttttg cctgtagccg ctgcctatgg ctggtatatg ggccgcagaa gtgcgcaaca
    24241 aaacaagcaa gatgaagcca accgcttgtc gcgtgattac gtagcggggg ttaacttcct
    24301 gcttagtaat caacaggata aagcggtaga cctgtttctc gatatgctta aagaggatac
    24361 aggcaccgtt gaagcccacc ttacgctcgg aaacctgttc cgttcgcgtg gcgaagttga
    24421 tcgcgctatt cgcatccatc agaccctaat ggaaagcgcc tcgctgacct atgaacagcg
    24481 tctgttggcg attcaacaac tagggcgtga ttacatggcc gccgggttat acgaccgcgc
    24541 ggaagacatg ttcaatcagc tgaccgatga aactgacttc cgcattggcg ctctgcaaca
    24601 gttgctacaa atctaccagg ctaccagtga gtggcagaaa gcaattgatg ttgccgaacg
    24661 cctggtgaag ctgggtaaag ataaacagcg cgtcgaaatt gcccatttct actgtgagtt
    24721 agccctgcag catatggcca gcgacgatct cgatcgtgcc atgaccttgc taaaaaaagg
    24781 ggcggcggca gataaaaaca gcgcccgcgt atccatcatg atgggacgcg tgtttatggc
    24841 gaaaggagaa tacgccaaag ccgtcgaaag tttgcaacgc gtgatatccc aggacagaga
    24901 actggtcagc gaaacgctgg aaatgctgca aacctgctat cagcagttgg gtaaaactgc
    24961 cgaatgggca gaattcctgc agcgtgcggt ggaagagaac accggtgccg atgctgaatt
    25021 gatgcttgct gatatcatcg aagcgcgcga cggtagtgag gccgcacagg tctatattac
    25081 gcgccagctt cagcgtcatc cgaccatgcg tgtgttccat aagttaatgg attaccactt
    25141 aaatgaagcg gaagaagggc gtgccaaaga gagcctgatg gtgctgcgtg acatggttgg
    25201 cgagaaggta cgtagtaagc ctcgttatcg ttgccagaaa tgtggtttta ccgcatacac
    25261 cctctactgg cattgtccgt cttgtcgggc ctggtcaacc attaaaccga ttcgcggtct
    25321 tgatggcctg taatttttaa aaaaaatccg actttagtta caacatacta attattaatg
    25381 ttccattgtg ctccggcaac gacggcgcag aaaaagcctg ccaggggaaa aatcgcaact
    25441 gttaattttt tatttccacg ggtagaatgc tcgccgttta cctgtttcgc gccacttccg
    25501 gtgcccatca tcaagaaggt ctggtcatga cgttaactgc ttcatcttct tcccgcgctg
    25561 ttacgaattc tcctgtggtt gttgcccttg attatcataa tcgtgatgac gcgctgtcct
    25621 ttgtcgacaa gatcgaccca cgcgattgtc gtctgaaggt cggcaaagag atgtttacat
    25681 tgtttgggcc acagtttgtg cgcgaacttc aacagcgtgg ttttgatatc tttcttgacc
    25741 tgaaattcca cgatattcct aacactgcag cgcacgctgt cgctgctgca gctgacttag
    25801 gcgtgtggat ggtgaatgtt catgcctctg gtggggcgcg tatgatgacc gcagcgcgtg
    25861 aggcactggt tccgtttggc aaagatgcac cgcttttgat tgctgtgaca gtgttgacca
    25921 gcatggaagc cagcgacctg gtcgatcttg gcatgacact gtcacctgca gattatgcag
    25981 aacgtctggc ggcactgacg caaaaatgtg gccttgatgg tgtggtgtgt tctgctcagg
    26041 aagctgtgcg ctttaaacag gtattcggtc aggagttcaa actggttacg ccgggcattc
    26101 gtccgcaggg gagtgatgct ggtgaccagc gccgcattat gacgccagaa caggcgttgg
    26161 cggctggtgt tgattatatg gtgattggtc gcccggtaac gcaatcggta gatccagcgc
    26221 agacgctgaa agcgatcaac gcctctttac agcggagtgc atgatgagtg attccaacag
    26281 ccgtctggtc tactcaacag aaaccggacg tattgatgag ccaaaagcgg ccccagtacg
    26341 ccctaaaggc gacggtgtgg tgcgtattca gcgtcagacc agtggacgta aaggtaaggg
    26401 cgtttgcctg attaccggtg tcgatctcga tgatgccgaa ctgacaaaac tggcagcgga
    26461 actgaagaaa aaatgcggct gcggcggagc agttaaagat ggaattattg aaatccaggg
    26521 cgataagcgt gatttattaa aatcactgct tgaagcgaaa gggatgaagg taaaactcgc
    26581 aggcggttaa cataaaaagc cacggatata tccgtggctt tcgaatattt tactgtgtgt
    26641 attattcagt tatttcgtac tgaacagaat caagcgtatt ataattgcga gtcgataatc
    26701 gacatttatt tacgattatt taccgacctg gtgaccaata acaccaccga cagctgcgcc
    26761 acctaatgta cccaacgtac tgccatcggt cagtactgca ccgcctaatg cccctgcacc
    26821 cgcgccgatt gcggtgttgc ggtcccgttt agaccagtta gaacaggcac tcagagacat
    26881 tgccagggta attgccagaa cagccgcggt catttttttg ctcgttacaa acataatact
    26941 ctctcctgaa tttatgattc acggaagtaa gctctctata actataatag ctaagaataa
    27001 gcctggtgaa attattccgt gaaatccgct cgcgcgcagg gttatatcac gcaggtgat
//
LOCUS       DAOFQD010000063        26811 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.063, whole genome shotgun sequence.
ACCESSION   DAOFQD010000063 DAOFQD010000000
VERSION     DAOFQD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.063"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..735)
                     /locus_tag="QBF46_002674"
     CDS             complement(<1..735)
                     /locus_tag="QBF46_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDQ6674838.1"
                     /translation="MSENKLFDNYEIIERNLLKTESNRNIPEVILTLKDGESYIIKHW
                     PRNTSSDEQVLESIWQHELRQLQRLKGYPGVGDYIVNPVESKKTNEGYYLVLNSDGRV
                     PTSYLLNKRTLSLPRKSHWLTRLKDPAVRIRFWKNICRITNAIGLLHAQGLLHRHLDE
                     NSILTYDYESDDYNDFQLTGFEWSIRMPTLTRAKIEPVGEHGKISTFATDWIDLGILI
                     ANFLKIDINEIKSLQFPAEYFISKYDL"
     gene            complement(1060..2289)
                     /locus_tag="QBF46_002675"
     CDS             complement(1060..2289)
                     /locus_tag="QBF46_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6674839.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLSKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSLLANGIDPQVHNNDKANAL
                     KNATEHTLQAVARKWLDEKVKTSGISQDHAEDIWRSLERNIFPGLGKVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFLMVLNNASVRMETRLLIEWQLLTWVRPGEAVRARWADIDTEKSMWNIPA
                     EFMKMKKPHKVPLSKEALRVLDSMKAISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTASEESGKFRTDVLEAALAHSKKDEIIAAYNRADYLAERVVLM
                     QWWSDYVQAQRLKAIAA"
     gene            complement(2455..2817)
                     /gene="ssrA"
                     /locus_tag="QBF46_002676"
     tmRNA           complement(2455..2817)
                     /gene="ssrA"
                     /locus_tag="QBF46_002676"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3032..3514)
                     /gene="smpB"
                     /locus_tag="QBF46_002677"
     CDS             complement(3032..3514)
                     /gene="smpB"
                     /locus_tag="QBF46_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDQ6674840.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            3685..4122
                     /gene="ratA"
                     /locus_tag="QBF46_002678"
     CDS             3685..4122
                     /gene="ratA"
                     /locus_tag="QBF46_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDQ6674841.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            4112..4402
                     /locus_tag="QBF46_002679"
     CDS             4112..4402
                     /locus_tag="QBF46_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDQ6674842.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(4464..4805)
                     /gene="bamE"
                     /locus_tag="QBF46_002680"
     CDS             complement(4464..4805)
                     /gene="bamE"
                     /locus_tag="QBF46_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDQ6674843.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(4954..6615)
                     /gene="recN"
                     /locus_tag="QBF46_002681"
     CDS             complement(4954..6615)
                     /gene="recN"
                     /locus_tag="QBF46_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDQ6674844.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(6701..7579)
                     /gene="nadK"
                     /locus_tag="QBF46_002682"
     CDS             complement(6701..7579)
                     /gene="nadK"
                     /locus_tag="QBF46_002682"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDQ6674845.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            7702..8295
                     /gene="grpE"
                     /locus_tag="QBF46_002683"
     CDS             7702..8295
                     /gene="grpE"
                     /locus_tag="QBF46_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDQ6674846.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(8350..9636)
                     /locus_tag="QBF46_002684"
     CDS             complement(8350..9636)
                     /locus_tag="QBF46_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDQ6674847.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(9657..10448)
                     /locus_tag="QBF46_002685"
     CDS             complement(9657..10448)
                     /locus_tag="QBF46_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDQ6674848.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            10615..11976
                     /gene="ffh"
                     /locus_tag="QBF46_002686"
     CDS             10615..11976
                     /gene="ffh"
                     /locus_tag="QBF46_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDQ6674849.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            12113..12361
                     /gene="rpsP"
                     /locus_tag="QBF46_002687"
     CDS             12113..12361
                     /gene="rpsP"
                     /locus_tag="QBF46_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDQ6674850.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            12380..12928
                     /gene="rimM"
                     /locus_tag="QBF46_002688"
     CDS             12380..12928
                     /gene="rimM"
                     /locus_tag="QBF46_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDQ6674851.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            12959..13726
                     /gene="trmD"
                     /locus_tag="QBF46_002689"
     CDS             12959..13726
                     /gene="trmD"
                     /locus_tag="QBF46_002689"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDQ6674852.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            13768..14115
                     /gene="rplS"
                     /locus_tag="QBF46_002690"
     CDS             13768..14115
                     /gene="rplS"
                     /locus_tag="QBF46_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDQ6674853.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(14191..14673)
                     /locus_tag="QBF46_002691"
     CDS             complement(14191..14673)
                     /locus_tag="QBF46_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDQ6674854.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(14689..15915)
                     /gene="dgcN"
                     /locus_tag="QBF46_002692"
     CDS             complement(14689..15915)
                     /gene="dgcN"
                     /locus_tag="QBF46_002692"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDQ6674855.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            complement(15905..16423)
                     /locus_tag="QBF46_002693"
     CDS             complement(15905..16423)
                     /locus_tag="QBF46_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDQ6674856.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            complement(16573..16938)
                     /locus_tag="QBF46_002694"
     CDS             complement(16573..16938)
                     /locus_tag="QBF46_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDQ6674857.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            17148..18218
                     /gene="aroF"
                     /locus_tag="QBF46_002695"
     CDS             17148..18218
                     /gene="aroF"
                     /locus_tag="QBF46_002695"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDQ6674858.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            18229..19350
                     /gene="tyrA"
                     /locus_tag="QBF46_002696"
     CDS             18229..19350
                     /gene="tyrA"
                     /locus_tag="QBF46_002696"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDQ6674859.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(19393..20553)
                     /gene="pheA"
                     /locus_tag="QBF46_002697"
     CDS             complement(19393..20553)
                     /gene="pheA"
                     /locus_tag="QBF46_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDQ6674860.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTCLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     misc_feature    complement(20575..20700)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(20652..20699)
                     /gene="pheL"
                     /locus_tag="QBF46_002698"
     CDS             complement(20652..20699)
                     /gene="pheL"
                     /locus_tag="QBF46_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDQ6674861.1"
                     /translation="MKHTPFFFAFFFIFP"
     gene            complement(20803..21144)
                     /gene="raiA"
                     /locus_tag="QBF46_002699"
     CDS             complement(20803..21144)
                     /gene="raiA"
                     /locus_tag="QBF46_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDQ6674862.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            complement(21415..22152)
                     /gene="bamD"
                     /locus_tag="QBF46_002700"
     CDS             complement(21415..22152)
                     /gene="bamD"
                     /locus_tag="QBF46_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDQ6674863.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            22287..23267
                     /gene="rluD"
                     /locus_tag="QBF46_002701"
     CDS             22287..23267
                     /gene="rluD"
                     /locus_tag="QBF46_002701"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDQ6674864.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPSKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            23264..23995
                     /gene="pgeF"
                     /locus_tag="QBF46_002702"
     CDS             23264..23995
                     /gene="pgeF"
                     /locus_tag="QBF46_002702"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDQ6674865.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVVENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYQADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            24125..26698
                     /gene="clpB"
                     /locus_tag="QBF46_002703"
     CDS             24125..26698
                     /gene="clpB"
                     /locus_tag="QBF46_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDQ6674866.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         6643 a         6222 c         6940 g         7006 t
ORIGIN      
        1 cagatcgtac ttagaaatga agtattctgc tgggaactgc agagatttaa tttcatttat
       61 atctattttt aaaaagtttg ctattaaaat tcctaaatcg atccaatctg tagcaaaagt
      121 tgatatttta ccgtgttcac caacaggctc aattttagct ctggtaagtg taggcattcg
      181 gattgaccac tcgaagccgg tcaattgaaa atcgttataa tcatctgatt cataatcata
      241 tgttagaatt gaattttcat ctaaatgtcg atgtaacaac ccttgcgcat gaagcaaacc
      301 aatagcattt gtaattctac agatattttt ccagaatctg attctaactg ccggatcttt
      361 taaacgggtg agccagtggc tttttcttgg gagagataat gttcttttat tcaataagta
      421 agaagtaggt actctaccat cagaatttaa aacaagatag tacccttcat ttgttttttt
      481 tgattcaaca ggatttacta tataatctcc tactccagga tatcccttta acctttgtag
      541 ttgtcgtaat tcatgttgcc aaatactttc aagaacttgt tcatcacttg aggtatttct
      601 tggccaatgt ttgataatgt aactctctcc atctttaagg gtaagaatta cttcaggaat
      661 atttctattt gattctgttt ttagtagatt tctttctata atctcataat tatcaaacag
      721 tttattttca ctcatagacc aaccttattc ataaatgctg tgtgaacttt acggatgaga
      781 attgtacatt taaatgctac cgccccaaca tatgcatgta tgctgtacat tgtatttatt
      841 gctaacataa aaaaacaata tatacaatga gttaagtagt gttgtttggt ttggatcaaa
      901 aaaaaagctt ctgaaattat ttgaccagct attgcttaat ctcagaagct aaaacagggc
      961 tttaattggt taatgctggg tgtatggttc taagttgttc gttgaaaata gagatgcaat
     1021 cctagtaatt acctttgttt attatgtgtt ttttaattat taagctgcaa tggcttttag
     1081 cctttgagct tgcacataat cactccacca ctgcatgaga accactcgtt ctgcaagata
     1141 atctgcgcga ttatatgcgg caattatttc atctttcttc gagtgagcaa gggccgcttc
     1201 taagacatct gtcctgaact tacccgactc ctctgaggcc gttcgtgcaa tagatctcat
     1261 tccatgggct acaagctctc caccaaagcc catacggatt atggccgcat tcgctgtttg
     1321 ttcatgcata tgattgagtg gagctttgat actggggaaa acccactctc tgtgcccact
     1381 gatggctttc attgaatcca agactcgcaa agcttctttg cttagtggaa ctttgtgagg
     1441 cttcttcatt ttcataaact cagccgggat gttccacatc gatttttcag tatctatatc
     1501 ggcccatctc gcacgaacgg cttcccctgg acgaacccag gtcagcaatt gccattcaat
     1561 cagcaatctt gtctccatac gaactgaggc attgtttaac accattagga agcggggtaa
     1621 ctcggaaggg ggtaatgctg gcatattctg ttttttaggc ttactgaacc gttgcccaag
     1681 gttgtcagcc ggattgaact cgataagttc ttcagtcgcg gcccagcgaa atatttcatt
     1741 taatcgggaa atgattcgcc gtaaggtttc gaggactcct cgttgctcaa tagggtcgag
     1801 atgttgcttt aagagtttgg gtcggatctc attgatagga accttaccca agccgggaaa
     1861 tatatttctc tccaagcttc gccagatgtc ttctgcatgg tcttgtgaga taccagaggt
     1921 ctttaccttc tcatccaacc atttccttgc cacggcttgg agagtgtgtt cagtagcatt
     1981 ctttaaggca ttagctttat cgttgttatg aacttgggga tcaatgccat tagcaagcaa
     2041 ggaaaggtat tcatctcgta aggctctggc tcttgccagt gtaaggtgag gataggtccc
     2101 aaggctcatt ttggttcttt ttttactcac tggcactgca tacctgaaat accaattctt
     2161 ctttcctcct ttcgagaggg gagcgattcg tagtatcaaa ccatctccgt caaacaagtt
     2221 gatttctttg tcggctggtt tggtgctttt gatttcagtg tcagtgagct tcttagcgat
     2281 ttttgccatt ttgggaccct cggtttttgg acccttctta gtgggtccca ttcagggtgc
     2341 catatgtgat agttctcagc aattctcact ggacgacaat agacgtaaaa aagcccgcaa
     2401 ggctgattcc atgcgggctt agtagacttc attgtacttc aaacaactaa aaagtggtgg
     2461 agctggcggg agttgaaccc gcgtccgaaa ttcctacatc ctcggtacta catgcttagt
     2521 cagtctttac attcgcttgc cagctgcgga cggacacgcc actaacaaac tagcctgatt
     2581 aagttttaac gcttcaaccc caggcagggc ttccacgcga tctcttttgg gtttgacctc
     2641 tcttgatccc cgtcctaaga gcggaggcta gggagagagg gctctaagca ggttattaag
     2701 ctgctaaagc gtagttttcg tcgtttgcga ctattttttg cggcttttta cgaggccaac
     2761 cgcccctcgg catgcacctt gggtttcgca aatcccgtcg aatccagaat cagccccaat
     2821 gtgtaaaggt aagtatacca gatttatgag cgccatgacc agcctcaatg gcgttatcgt
     2881 taaagattta gcacccatgt agcctgattt ttattcgatt aagcaatggg atggcaacat
     2941 ttgtgtcgga tgtgatagcc aataagatgt tcattcgcgc cgccggagag ggaggcgcgg
     3001 tgaggaactg gtcaataatt ggagtgcagg tttaacggtg ggcgtttttc atgatacgcg
     3061 ctttatccac ctgccattcg cgctctttga tatctgaacg tttatcgtgc tgtttcttac
     3121 ctttggcgac gccgattttc actttgcacc aggcattttt ccagtacagg gagagcgcca
     3181 ctacggtata gccttctcga ttgacgcgac cgtacaatga gtccagttcg cgctggttga
     3241 gaagtaactt gcgggtacgg gtaggatcgc acaccacatg cgtggaggcc actgccattg
     3301 gcgtgatgtt tgcgccaaac aggaacgctt ctccgtcacg cagaaggaca tagctgtcgc
     3361 tgatattggc ttttcctgcg cgcagggatt taacttccca gccttgcagg gcaagtcccg
     3421 cttcgaactc ttcttcgata aagtattcgt gacgggcgcg cttgttaagc gcgatggtcg
     3481 ctgaaccagg tttatgtgct tttttcttcg tcataagcgt cgtgaatcat cggtaatctg
     3541 aaatcgaaaa caccccatat caatcctgcg ggggacaagg ctctatctta gcatgaaccc
     3601 gatgttaccc agcgccggga tagcgttttt tttacagcag gataaatgat attatttgtt
     3661 ggatttttgt tgatggaaat tgttatgcct cagattagcc ggaccgcact ggtaccctac
     3721 agcgcggagc aaatgtatca gttagtgaat gacgttcagt cttatcctca gtttttgccg
     3781 ggttgtaccg gaagtcggat tctggagtcc actcctgggc agatgactgc ggcggtagat
     3841 gtctctaagg ctgggatcag caaaacgttt actacccgca accagttgac cagtaaccaa
     3901 agtattctta tgaatctggt ggatgggccg ttcaagaaat tgattggtgg atggaagttt
     3961 acgccgctga gccaggaggc gtgtcgtatc gagtttcatc tcgactttga gtttaccaat
     4021 aagttgattg aactcgcctt tggtcgcgtg tttaaagagc tggcgtctaa tatggtccag
     4081 gcttttacgg ttcgtgcgaa agaggtttac agtgccaggt aaaattgccg ttgaggtggc
     4141 ttatgcgcta cctgagaagc agtacctgca gcgcgtgacg ctgcaggagg gcgcgacggt
     4201 tgaagaagct attcgcgcca gtggcttgct ggaattgcgt accgatatcg atttaactaa
     4261 aaacaaagtc ggcatttaca gccgtccggc aaaactaagc gatagtgtgc atgatggcga
     4321 tcgggtggag atttatcgcc ctctcattgc cgatccgaaa gagcttcgca ggcaacgagc
     4381 agaaaaatca gcgaataaat aacagacaga aaaaaggtgc tcattgagca ccttttttaa
     4441 cgtctttgag agcaacttta ttattagtta ccactcagcg caggtttgtt atcaatattg
     4501 gtcaacacac cgctactgtt aaaggtcagc gtcagcgttt gctgcgttac accttcatga
     4561 cctggttgct ggcggaagac atagaaccag gtattcgtac caaatggatc ggacatcagc
     4621 ggtgtaccca atgcgtacgc aacttgttgt tgcgtcatgc caacacgtat tttggatacg
     4681 tcgttagcgg tcagatagtt cccctggttg atgtcaggac ggtaaaccac tcgctccaga
     4741 gtggaacagc ctgcggtcaa catcaatagt actgctgctg cagcagtcag cgttttacag
     4801 cgcatagtga tttgattcct tttcgggccc gagcagtacg tggctcatct gtaatatgcc
     4861 gatgataata gacctttcat cactttaaaa ccttttgctt tccggtctta cggcgttttg
     4921 ctgtttactc tgaccgtgaa gcaggaaaaa agtttacgct gcaagcagtt ctttcgcatt
     4981 cgccagtgta ttacgtgtga cttcactgcc accaagcagg cgcgccagct cttgtaaccg
     5041 cgctttttta ttcagggatt gcatatgcgt ttctgtcatc gcaccatcgg tttctttgct
     5101 gacaaaatag tgttgatgac cacatcccgc gacttgtggc aggtgggtaa cacacatcac
     5161 ctgagttgat tcgccgagtt gacgcagcag tttgccgaca actgccgctg ttggaccgct
     5221 aatccctaca tccacttcat caaaaatcag tgccggggtt tccattttac gcgccgtgat
     5281 gacctggatt gccagtgcga tgcgggacaa ttcaccaccg gatgcgactt tggcaatagg
     5341 ctgcattggc tgacctgggt tggtggttac ccgaaactca atacgatcgg caccgtcagc
     5401 gcccaggtga tgctcgtcaa atttaacatc gatcgtaaac tgcccatgcg gcattgagag
     5461 cgcatgcata ctgtcggtga tcagctgtgc aagttcttct gcatattgct ggcgttgttg
     5521 gtgtaatgcg cgcgcgattt ccagtgcctg ctgatgatgt ttcgttaccg ccagcgcaag
     5581 cgtttcttgt gagtcggcct gatcgtccag ttgctgctgt tcttccagta gcgactggta
     5641 atactgtggc aatgcctcag ggctgacgtg atgtttacgt gccagcgaaa tctgttttga
     5701 gatgcgctgt tcaagttcaa atagtcggtt gggatcgaga tccagacgat cgcagtagtg
     5761 gcgcagttca tcgctggctt cagcaatctg gatggtagct tcttccagca tatcaagtac
     5821 gccggacagt ttgctgtcca tgccaatcaa ttcgctcacc agttgtttag ccgtgtaaag
     5881 ctgactttgc aggtttgcgt cttcaccgtc ggccattaat gccaatgcat tctggctggt
     5941 ggtcagcaat tgaccgctgt tcgccagacg tttgtactct tcgtcgattt gttcaaactc
     6001 tccgggctgc ggattaaatt cgttaagttc ttttaattgg tattgcagca gttccgcacg
     6061 ggcagcgcgt tcctgactta actgttgatg atgcgcgagg tcacggcagc tttgatgcca
     6121 caactgataa cgtgctgtca tttcctggag tagagaggtt tcattggcat agccatcaag
     6181 caggaacttt tggtgctcgg gtttggtgag taactgatga gcgtgctgac catggatctg
     6241 aatcagcaac tgacccagtt cgcgcagttg tgacagagga acagctgtac cgttgatgaa
     6301 accacgggag cgaccatcgc tgctgatcac gcgacgaagc aaacattcat gcccgtcttc
     6361 aagctggttt tcttccagcc agcgcagagc cgctggcgta tctttcagag aaaaacgggc
     6421 gcacaggtca gcgcgagcag cgccggtacg caccatgtcg gcttcagcgc gaccaccgag
     6481 acaaagaccg agggcatcta ttgcaataga tttacccgcg ccggtctcgc cagttattac
     6541 ggtcatgccg ctatgaaaat caatctcaag ctcacgaacg atagcaaagt tgctgatggt
     6601 cagttgtgcc aacatagtcg ttttcctgta tgaaaaacca ttactgttat tgtgtacagt
     6661 ataaactggt tttatataca gtaaagaggc tggcgtaaaa ttagaataat ttttttgacc
     6721 agccgagctt ggtgcttaat gtgttgaaat aactgtaatc tttcggatga atcagattca
     6781 gatggtaatc acagcgacga atcaggacat cttcaccttc ctgaatcggc agtgctatct
     6841 ggctgtcgca actgatttcc aggtcgttac ggcgatgcga aaaacgcaga cggatcgtgc
     6901 tgctgctgtt tatgaccagt ggtcgtgctg acaacgtatg cgggaacatg ggcaccaggg
     6961 taatcgcatc cagagagggg gtcagaatag gaccgcctgc agagagggaa taggcggtgg
     7021 agcctgttgg cgtcgaaata attagtccat cagatcgctg agaaaacgca aagatctcgt
     7081 cgatatacac ttcgaactca atcatatgcg ccactttgcc tggatgaagc accacttcat
     7141 ttatcgcggt gctgatgcgt ttctggcaat cttgctgaca gacttgcgct tccagcaaaa
     7201 aacgtttctc gctgatgtag tggccttcca gcacatcggc taactgttgc tgggcgttat
     7261 cggggtcaag gtcagtcagg aaacccaggt tgccacggtt gattccaata actttaatat
     7321 cgtaacgggc gagtgtgcgt gccgcgccca gcatattacc gtcgccacca acgactaccg
     7381 cgagatcagc tagttgccca atctccgcga gcgtgccagt tttcacattc ttcagttgca
     7441 gttcgtgagc gatttgttgc tcaacgatga cctcgtaacc ttttgtgcac agccagcggt
     7501 agagcatttc atgtgttgtc agtgcagtgg ggtgccgtgg gtgtcccaca atgccaatac
     7561 acttgaaatg attattcatt tttccgaggt ccttgttgcg aagattgatg acaatgtgag
     7621 tgcttccctt gaaaccctga aactgatccc cataataagc gaagttagcg agatgaatgc
     7681 gaaaaaaacg cggagaaatt catgagtagt aaagaacaga aaacgcctga ggggcaagcc
     7741 ccggaagaaa ttatcatgga tcagcacgaa gagattgagg cagttgagcc agaagcttct
     7801 gctgagcagg tggatccgcg cgatgaaaaa attgcgaatc tcgaagctca gctggctgaa
     7861 gcccagaccc gtgaacgtga cggcattttg cgtgtaaaag ccgaaatgga aaacctgcgt
     7921 cgacgtactg aactggatat tgaaaaagcc cacaaattcg cgctggagaa attcatcaac
     7981 gaattgctgc cggtgattga tagcctggat cgtgcgctgg aagtggctga taaagctaac
     8041 ccggatatgt ccgcgatggt tgaaggcatt gagctgacgc tgaagtcgat gctggatgtt
     8101 gtgcgtaagt ttggtgttga agtgatcgcc gaaactaacg tgccgctgga cccgaatgtg
     8161 catcaggcta tcgcaatggt ggaatctgat gacgttgcgc caggtaacgt actgggcatt
     8221 atgcagaagg gttatacgct gaatggtcgt acgattcgtg cggcgatggt aactgtagcg
     8281 aaagcaaaag cgtaatttct gttttcgtaa taattcacgg ccctgcatgc gaatgcaggg
     8341 ccgttttcgt tactccgcca cactctcgcg cagcggtttc acaggaaaaa cttttacctg
     8401 cttaatcata ttgtcctgta cgtcgagaat atctatatcg tactcgccaa tacgcacgcg
     8461 ggtgcctgcg acagggatct cttccagtgc ctcaagaatg acgccattaa ccgtgcgggc
     8521 atcatcttcc ggtagatgcc agttaaaggc tttgttgatt tcccgcacgt tggcggtgcc
     8581 atcgataatc accgaaccgt cgttttgcgg cgtgacctct tcggcaagtg ttggcgacat
     8641 cgacgtagtg aaatcgccga caatctcttc cagaatatct tcaaccgtca ccagcccctg
     8701 aatgtctcca tactcgttga cgaccaggcc gactttcttt ttgttgcgct gaaactttac
     8761 caactgcgtg ctgagcggcg taccttccgg cacaaaatag atctcgtccg cggcgcgcag
     8821 catggtttct ttggtgaact cttttttctc cgacatcaac cgccaggctt cacgtactcg
     8881 cagcatactg atggcgtcgt ccagcgaatc acggtagagc acgatgcgcc cgtgaggtga
     8941 gtgggagagt tggcgcagaa tcgatttcca gtcatcgttg atatcaatac cgataatttc
     9001 actgcgcggc accatgatgt catcaacggt cattttttcc agatcgagca ccgacagcag
     9061 catatcctga ttgcgacggg aaatttgtga gcgtgattcg tgcacgatag tgcgcaactc
     9121 ttctttgctc aaagagccgc taaccacgat atcggttttg atacccatca tgcgcatcag
     9181 catacgggtg atagcattca gcaaccagac cagcggcatc atcaaaattt gcagcggagc
     9241 cagcagaaaa ctactcggat aagcgacttt ttccgggtac agcgcggcaa tggttttcgg
     9301 caatacctca gcaaatacca gtacgacaaa agtcagcaca ccagtcgcaa ttgccacgcc
     9361 cgcatcgccg tacaaacgca tcccaacaat agtgccgagc gcggaggcaa gaatattgac
     9421 caggttattg ccgattaaca ccaggcttat caggcggtct ggcttacgca gcaatttttc
     9481 gacgcgtttg gccgagcgat taccctgttt cgccatatgt cgcagacgat agcggttgag
     9541 ggtcatcatt ccggtttcgg acccggaaaa ataggctgaa atgaccacca tgatgatcag
     9601 aataatgatc aacgtagtag tagaaatgtg ttccagggga aactcctttt ctgggtttag
     9661 ctgattaact gctggacaat tcggctgccg aagtaggcca gtgtcagaat taccgcgccc
     9721 gcaacgttaa accagacgac gcggcgtcca cgccatcctt catggtaatg tccccacagc
     9781 agcacaatat agacaaacca cgccacgata gagagcacag ccttgtcgat attttccatg
     9841 ctaaataggt tgtgcatgta gaacaggcca gtgcaaagcg tgagcgttag cagcaccacg
     9901 ccaatctgcg tgatgtggaa cattttacgc tcgatactca tcaatggcgg catttcctgg
     9961 ttaaatgcca gcttcttgtt cttcagttgg taatcaatcc acgccagttg cagcgcgtac
    10021 agggcggcga taattagcgt ggcataggaa aagagcgata agccaatgtg caccagcatc
    10081 ccaggcgttg cttccagatg ggtgatgtat tcattgggca tgaaggtcgc cagcgccagg
    10141 ttgataagcg caaaggcata gacaatgggt agcagcaacc agccacgatt gcgagaagcc
    10201 acaatggtca ttaccgtaca gatcatcaaa ctgaccaatg aaccaacgtt cagcaggctg
    10261 aggttttgtc cgctatcacc gtcgggcagg atgcgggctt ccagagcgat tgcgtggcag
    10321 accagcgcaa tgaccgcaga aataatagcc atgcgccgcc agccgccgtt tttttgcagc
    10381 agaccgggaa caatcagcgc aagactgacg gagtaggcga caagcgcgag cagagcaaaa
    10441 acgggcatag tgatgtcgac agtttgagca ataagaaaga gaagcagtat agcgtcaggt
    10501 gaacgctgct ccaaccgttg cataacaaca aagacgcctt catgttatac tgcggcaaaa
    10561 tactgatgat gtgtcgcgat tgcggccaac cgtttccacc ccaggcgaga gacaatgttt
    10621 gataatttaa ccgatcgttt gtcgcgcacg ctgcgcaata tcagtggccg tggacgcctc
    10681 actgaagaca acgtaaaaga tacgctgcgc gaagtgcgca tggcgctgct ggaggcggat
    10741 gtcgctctgc cggtagtgcg tgagtttatc aatcgcgtaa aagagaaagc ggttggtcat
    10801 gaagttaata agagcctgac gccggggcag gagttcgtca aaatcgtccg taacgaactg
    10861 gttgcggcga tgggcgaaga gaaccagacc ctgaacctgg cggcgcaacc gcctgcggtc
    10921 gtgctgatgg cgggcctgca aggtgccggt aaaacaacca gcgttggtaa actcggtaag
    10981 ttcctgcgcg agaagcacaa gaagaaagtg ctggtggttt ctgccgacgt ttatcgcccg
    11041 gcggcaatca aacagcttga gacgctggca gagcaggtgg gcgttgattt cttcccttct
    11101 gatgttggtc agaagccggt agatatcgtt aacgcggcgc tgaaagaagc caaactgaaa
    11161 ttctacgacg tgctgctggt ggataccgct ggtcgtctgc acgttgacga agcgatgatg
    11221 gacgagatca aacaagtcca tgcgtcgatt aacccggttg aaaccctgtt tgtggttgac
    11281 gccatgaccg gtcaggatgc ggccaatacg gcaaaagcat tcaatgaagc gttaccgctt
    11341 accggcgtag tgttgaccaa agtggacggc gatgcccgcg gcggtgcggc gctctctatt
    11401 cgtcacatca ctggcaaacc gatcaagttt ctcggtgttg gcgagaaaac tgaggcgctg
    11461 gagccgttcc atccggaccg catcgcgtcg cgtattctcg gcatgggcga cgtactgtcg
    11521 ctgatcgaag atatcgaaag caaagttgac cgcgcgcagg cagagaaatt agccagcaag
    11581 ctgaaaaaag gcgacggctt cgatctcaac gattttcttg agcaactgcg ccagatgaaa
    11641 aatatgggcg gcatggctag cctgatgggc aagctgccgg gcatggggca gatcccggat
    11701 aatgttaagt cgcagatgga cgataaagtg ctggtgcgta tggaagccat catcaactcg
    11761 atgacgatga aagagcgcgc taagccagaa atcatcaaag gttcgcgtaa gcgtcgtatt
    11821 gctgccggtt gcggtatgca ggtgcaggac gttaaccgtc ttctgaaaca gttcgacgac
    11881 atgcagcgca tgatgaagaa aatgaagaag ggcggaatgg cgaagatgat gagaagcatg
    11941 aaggggatga tgccgccggg atttcctggt cgctaaatga ccttatgcga cagagttatt
    12001 aactgctgat tgcattttct ccagaaatca gtaaaatttt cgggctttta atatgacacc
    12061 ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg ttatggtaac
    12121 tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg ttgtcgctga
    12181 cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca acccaatcgc
    12241 tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact gggttggcca
    12301 gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca aagcagctta
    12361 atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg ttgatcccat
    12421 cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag tgttttcttc
    12481 caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga aggcgggtca
    12541 gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga tcatcaagct
    12601 gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa ttgtcgtgga
    12661 ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc tgatgggctg
    12721 ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga tggaaaccgg
    12781 atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta tcaaggaacg
    12841 tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta ctcgttcaat
    12901 cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag acggcgctat
    12961 gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg attacggggt
    13021 aactggccgg gcagttaaaa atggcctgct gagcatccag agctggagtc ctcgcgattt
    13081 cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac cggggatgtt
    13141 aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg cgggtgaagg
    13201 cgcaaaggtg atttatcttt caccacaggg acgcaagctt gatcaagcgg gcgtcagcga
    13261 actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta tagatgagcg
    13321 cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc tcagtggtgg
    13381 tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg gagtactggg
    13441 acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt gcccgcacta
    13501 tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt cgggcaacca
    13561 tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc ttagaagacc
    13621 tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg cggagttcaa
    13681 aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc ccgagatatc
    13741 agtttaccca ggataagaga ttaaattatg agcaacatta ttaagcaact tgaacaagag
    13801 cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt gaaagtatgg
    13861 gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat cgctattcgt
    13921 aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga aggcgttgag
    13981 cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg tcgtggtgct
    14041 gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc tcgtatcaaa
    14101 gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct ggccaattgg
    14161 ctggcccttt tttatctgtt tgctggtgtt ttaaggggta gtaatcacca ctgcgacgcg
    14221 gcggttctcg gcgcggccct gggcggtctt gttactggct atgggatatt tttttcctaa
    14281 accctgtgtg gtgagattgc tgcgtggaat ttgtccaccc atagcccatg catcggccac
    14341 gacattcgcc cgtttcaatg ataagccttc gttgtaactg tcttcaccat agttatcggt
    14401 gtgtccatcc atacgggcat gtgttagccc tgtcgaggcc aatttggctg ccatggtttg
    14461 gatctgttgc tggctttccg ggagcaattt gtagtcattt tttgcgaaca gaatggcatc
    14521 tgataagccc agcgaccagt cgccggcgga ttcagtaaat ccataagatt gcatagcggc
    14581 gacttgctca ggagtaaact ttccctgagg ggactggcag ccagtcagta ttagtgaggt
    14641 gaaaatcagg ggtgctacca ggtgctttat catatcgata tatccttgtt atctcaccag
    14701 cttttcggca cgctggtgtt tggcctgata catattgtga tcggcaagct cttgtaattt
    14761 ttccgcagag gcgtgctcaa tggtcatcgc gtaaccaatg cttaatgtca tggtggtctg
    14821 atgaccatta tgaagatcaa acgggagatt aaagatttgt gtcagtgctg agcatatctg
    14881 ctgcacttca gattctgact gtacatcata gagcaccata gcgaattcat cgccgcccag
    14941 gcggtatgct ttatgtcgca gcccgccaac ttcagctaac cgttttgcga tttcaatcaa
    15001 gactctatcg cccgtcgcat gaccccaggt atcattgatg tatttgaaat tatcgccatc
    15061 aagaaatagt aacgccgacg tttttcgggc atcggaattg ttcatcaacg tgttgatgcc
    15121 gctacgaaac gctgcgcggt tagccagccc ggttaatggg tcatgtagcg cggtacgtag
    15181 aagctgcgca tttttagcct gtaaacgaag ctgccactct tccatttcat ccagcagact
    15241 gttgaagtcg agagcgaagc ggtgaaactc agcgatacgt tcttccgaaa ctcgtcggga
    15301 aaaattgcgg ttggaacgca catcatgtac gacatcggtg atatttttca gtgcttccac
    15361 caggccattg tgcaaatggc gggtgagggt aattgcgatg cctgatgcca gcagaataca
    15421 accggtcagt acggcgagcg aaaaccagat aaaatggctg attgaactgt cgcgagcggt
    15481 taagcgtact tcgccaatgg tttcaccatt gtgacgaatc ggctgaatga tgggggcggg
    15541 gaagagccag tggcttatga aattgctgaa agtatcgcct ggatccttac gggtgtaatg
    15601 ccaggacgcc agaatatttt gctgcttatc acgtacttct gcagttgaaa attgcccttg
    15661 ctggcccagc gctgccagtg tttcagttgc tgcagggcca tcggcaaaaa cgacagctgc
    15721 ttccagactg taagtcattg ttgctgctgt cagtgccagg tttttttgcg cgtactgttt
    15781 cagggtcagc accgaggtca cggaaagcag caaccagatc agcatcatag tgataaatat
    15841 gctggtgata ctgatgttgc gtaatgctct tttaaacgtg gggcgcttat taagagaatt
    15901 atcgttatcc atcatttttc ttccgtgcga gcattaagac atccgggttg acctttacac
    15961 cgctacgcga taaggcatcc aggtttacgg caaatctgac gtcattattg tggatgatca
    16021 gacaaaaggc gctaccaata atgcactcgg tattttgttc ggcaattaat aataacgcct
    16081 ttgacggata ttgttcggtt aattccatct ggaatgtcgg tgactcgttg ccaaaataaa
    16141 aaccattgca gcctgaaatc atcgcctctt gttgagtgtg aatgatgaca ggcagataag
    16201 gcaaagatgt cgcggcattc tcttgtaggg cggtactaaa acgtgaagat gaaaagatac
    16261 ataactttgg tggccctgat agcgcaggcc agcgggtgta actgacaata ccggaaacca
    16321 tcatacgcac gtttttcgca acgtcagaag gctcctgagc gagtattggt gctcccgtca
    16381 gcaggagaat cagaagtaag aacagtcggt gagaaaaacg cattcaggga tccccaccat
    16441 cttcgctcga aatgcatcga tattttttca gaatgctcat taaaatatca ttttttcatg
    16501 cgatcgtcat caatgtgaga aataaaacac aatgagtaaa atcttatgtt taatttattt
    16561 gtggggtttt aattaattta atcgtattgt gctggcattt tcatcagccc ctttttgcca
    16621 tacttcatgc agttggctgg cattttcaac attgttgcag ctggcaggaa agcttttacc
    16681 ggaaagtcct cgggcataag taaaatcggt ctggcaagtt ttcttttgtc cttcggcata
    16741 tcctttaagg tatagaccgc gatccgcctg tgaatcgcta aatgcatcgt catcttttat
    16801 ggcgctgccc gatatcgcat cttccatacc aacatcatac cagtcggtgc tggtcaaggt
    16861 tggcgcgtga gtataaggat caatctgaca tccgctaacc agtaaagcca acaagggggc
    16921 gataaacttt ttcatcattt ctttctcctt tttcaaagca tagcggattg ttttcaaagg
    16981 gagtgtaaat ttatctatac agaagtaagg gttgaaatcg cgactaaatt gcctgtgtaa
    17041 ataaaaatgt acgaaatatg gattgaaaac tttactttat gtgttatcgt tacgtcatcc
    17101 tcgctgagga tcaactatcg caaacgagca taaacaggat cgccatcatg caaaaagacg
    17161 cgctgaataa cgtacatatt accgacgaac aggttttaat gactccggaa caactgaagg
    17221 ccgcttttcc attgagcctg caacaagaag cccagattgc tgactcgcgt aaaaccattt
    17281 cagatattat cgccgggcgc gatcctcgtc tgctggtagt atgtggtcct tgttccattc
    17341 atgatccgga aactgctctg gaatatgctc gtcgatttaa agcccttgcc gcagaggtca
    17401 gcgatagcct ctatctggta atgcgcgtct attttgaaaa accccgtacc actgtcggct
    17461 ggaaagggtt aattaacgat ccccatatgg atggctcttt tgatgtagaa gccgggctgc
    17521 agatcgcgcg taaattgctg cttgagctgg tgaatatggg actgccactg gcgacggaag
    17581 cgttagatcc gaatagcccg caatacctgg gcgatctgtt tagctggtca gcaattggtg
    17641 ctcgtacaac ggaatcgcaa actcaccgtg aaatggcctc cgggctttcc atgccggttg
    17701 gttttaaaaa cggcaccgac ggcagtctgg caacagcaat taacgctatg cgcgccgccg
    17761 cccagccgca ccgttttgtt ggcattaacc aggcagggca ggttgcgttg ctacaaactc
    17821 aggggaatcc ggacggccat gtgatcctgc gcggtggtaa agcgccgaac tatagccctg
    17881 cggatgttgc gcaatgtgaa aaagagatgg aacaggcggg actgcgcccg tctctgatgg
    17941 tagattgcag ccacggtaat tccaataaag attatcgccg tcagcctgcg gtggcagaat
    18001 ccgtggttgc tcaaatcaaa gatggcaatc gctcaattat tggtctgatg atcgaaagta
    18061 atatccacga gggcaatcag tcttccgagc aaccgcgcag tgaaatgaaa tacggtgtat
    18121 ccgtaaccga tgcctgcatt agctgggaaa tgaccgatgc cttgctgcgt gaaattcatc
    18181 aggatctgaa cgggcagctg acggctcgcg tggcttaaga ggtttattat ggttgctgaa
    18241 ttgaccgcat tacgcgatca aattgatgaa gtcgataaag cgctgctgaa tttattagcg
    18301 aagcgtctgg aactggttgc tgaagtgggc gaggtgaaaa gccgctttgg actgcctatt
    18361 tatgttccgg agcgcgaggc atctatgttg gcctcgcggc gcgcagaggc ggaagctctg
    18421 ggtgtaccgc cagatctgat tgaggatgtt ttgcgtcggg tgatgcgtga atcttactcc
    18481 agtgaaaacg acaaaggatt taaaacactt tgtccgtcac tgcgtccggt ggttatcgtc
    18541 ggcggtggcg gtcagatggg acgcctgttc gagaagatgc tgacactatc gggttatcag
    18601 gtgcggattc tggagcaaca tgactgggat cgagcggctg atattgttgc cgatgccgga
    18661 atggtgattg ttagtgtgcc aatccacgtt actgagcaag ttatcggcaa attaccgcct
    18721 ttaccgaaag attgtattct ggttgatctg gcatcagtga aaaatggacc attacaggcc
    18781 atgctggcgg cgcacgatgg cccggtactg gggttacacc cgatgttcgg cccggacagc
    18841 ggtagcctgg caaagcaagt tgtggtctgg tgtgatggac gtaagccgga agcataccaa
    18901 tggtttctgg agcaaattca ggtctggggc gctcggctgc atcgtattag cgctgtcgag
    18961 cacgatcaga atatggcgtt tattcaggct ctgcgccact ttgctacttt tgcttatggg
    19021 ctgcatctgg cagaagaaaa tgttcagctt gagcaacttc tggcgctctc ttcgccgatt
    19081 taccgccttg agctggcgat ggtcgggcga ctgtttgctc aggatccgca gctttatgcc
    19141 gacattatta tgtcgtcaga gcgtaatctg gcgttaatca aacgttacta taagcgtttc
    19201 ggcgaggcga ttgagttgct ggagcagggc gataagcagg cgtttattga cagtttccgc
    19261 aaggtggagc actggttcgg cgattacgca cagcgttttc agagtgaaag ccgcgtgtta
    19321 ttgcgtcagg cgaatgacaa ccgccagtaa taatccagtg ccggatgatt cacatcatcc
    19381 ggcacctttt catcaggttg gatcaacagg cactacgttc tcacttgggt aacagcccaa
    19441 taccttcatt gaacgggtaa tttcccctaa ctctttcaat gctttttgca tttccgctga
    19501 ttcaagattg gcctgaatat ccagataaaa catctcttcc catggattac cgtgaatcgg
    19561 gcgtgattcc agacgggtca taatcagatt gtggttgcgc agcaccagca acgcttcaac
    19621 cagcgcaccg gcttgttgcc cggtcgccat taacaacgtc gttttcgccg gaacctgatc
    19681 agacacgtta atggctttac gcgccaacac cacaaatcgg gtgaagtttt gtcgctgatt
    19741 cgcttcaata cgctccagta cctgcaaacc gtacaaagtg ccgccagctt cgcttcccaa
    19801 cgcagcaaca tgcggtgatt ttgcctgtgc aaccttttcc attgccgcag acgtactttc
    19861 ggtatattca atcttccagt gcggataacg attaaggaat ttgctgcatt gctggaacgg
    19921 ctgtggatga ctgtagaccg tgttgatggt agataaatct gtggtgccgg aaaccagcag
    19981 gcagtggtcg atggtaaggg tcatttcgcc cacaatcgac aagcaggtat gttgcagcag
    20041 atcgtaaacg tcgtttatgg caccggagct ggtattttca atcggtacga cggcatagtc
    20101 ggcctggccg gtttccacct gattaaaaat atcggcaaat ttggcgcagc cactttcaat
    20161 gaattgctca aagtgacggg cagcgtactg acgagcggca agatgtgaat aggagccttt
    20221 ggggccgaga aaagcgatgc gtgctgagtg cggattaatt ttattgagat gttgttggag
    20281 caaagcctgc tgagttaata cggaatcttc aatgatgagc tggaacaggc gagtaatgta
    20341 atgggcgtcc agatggtgcg ctttaccgag cgtaattaat ctttccagta aatcgcgttc
    20401 acgatcaata tcacgtaccg ggcgatgcga gagcagtttg gcttttccca cctcgacggc
    20461 cagttcgcgc cgctctgcga gtaatgctaa taatttttca tccagcgcgc tgattttctc
    20521 tcgcagcgcc agtaacgggt tttccgatgt catagtgttg cctttttgtt atcaataaaa
    20581 aaggcccccc gatttgggag gccttattgt tcgtcttcgc attctgtttc acacgacgaa
    20641 acgcctccct ttcaggggaa gataaaaaag aatgcgaaga aaaacggtgt gtgtttcatg
    20701 tttgtttcct taagtgactt tagtacagta cccgtactgt tttcacgctg tcaacaaaaa
    20761 acgcgcccga aggcgcgtta gcgatacact caatataaag gactactctt cttcaacttc
    20821 ttcgacgaag ttggcgtctt tcaccgatgt tgcggcacga cgtgcttcgc ctttgtgctg
    20881 cagtttattg agctgccgtt ccagcttgtt gatcaattcg ttaattgcgg tgtacatatc
    20941 ttcatgttta ccactggcaa ccagaacgcc gttaggtgta ttgattgtgg cgtcagcaac
    21001 aaacccttgt ggctctttgg acagaatgat atgtggatta atcagatgtg tttgccattt
    21061 ttccagtttg gcgagacggt ctgcgacatg ttggcggatg gccggagtaa tttccatttg
    21121 tttgctggta atgttcattg tcataaattt tacctcttgt cttcccgtct tggtgaattc
    21181 agcataccgt tcctgatgtc aaaatgtgtg atgaaaatct cataattttg tcactttttg
    21241 tcaacgaatc ttttttgtga gaaatcgcag gaagaggtgt ttttttactt gaggaatatc
    21301 tcaaagcgag ccatatttga tgagatcgat agcgactaaa tcgcttcagt ttcacaactg
    21361 acagaataaa aaaacggcag ctcaagggct gccgttttgt gtttcaggtt tctgttatgt
    21421 attgctgctg tttgcggcga tgattttcgc tactttttca gcttgcgcat tcatctgcat
    21481 ctgacggtat gcattttcca tcagcggcag cgcatcacgc gtagcctggg tatccgggta
    21541 gtcgcgcaac atgccttcta cgcggttaac gacggcaacc catgcgccac gttctgtata
    21601 gtactcggcc acggagtatt catatttcgc cagacgatct ttcaggaata ccagacgttt
    21661 ggtggcatcg gtggtgtact gactgttcgg atagccgcgc accagtttgg aaaagtcact
    21721 aaacgcagct cgtgcatgtt gaggatcgcg atcgctacga tcgacgccaa agaacccttg
    21781 cagcgcactg tcatccagcg ccatattggt caggccacgc atgtacatga cataatcgat
    21841 attcggatgg gtcgggttaa ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa
    21901 atcggcgttt ttatagtagg cgtagatgag atccagctgc acctgctgcg aatacggacc
    21961 aaacggatag cgattatcta acgcttccag ttgcgttatt gcctgtctcc agttaccgtc
    22021 ctgcagcttt tgttgtgcag tcgcgtaaat ttcatttggc ggattatcag gtacttcttc
    22081 ctttgacccc gagcaacccg ccaaaaacag gcttagtgtg gcggctgcca ccagatattt
    22141 catgcgcgtc atgacgtttt gactttcctc aaaatgtaat acgggagatt ctctgttcct
    22201 gctcccggtt aagaccagct acaatagcac actatattaa acggcaaagc cgtaaaaccc
    22261 caacgataaa cgaagaagca gtatatatgg cacaacgagt acagctcact gcaacggtgt
    22321 ccgaaaacca actcggtcaa cgcttagatc aggctttggc cgaaatgttc ccggattatt
    22381 cacgttcgcg aataaaagaa tggatcctcg accagcgagt gctggttaac ggcaaagttt
    22441 gtgataagcc gaaagaaaaa gtattgggtg gcgagcaggt tgccatcaac gctgagattg
    22501 aagaagaagc gcgttttgaa ccgcaggata tcccgctgga tatcgtctat gaagatgaag
    22561 atatcatcgt cattaataaa ccacgcgacc tggtagtaca tcctggcgcg ggtaacccgg
    22621 atggcacggt actgaatgcg ttgcttcatt actatccgcc cattgccgat gtaccgcgtg
    22681 cgggcatcgt ccatcgtctg gataaagaca ccactggcct gatggttgtg gcaaaaaccg
    22741 ttccggctca gacgcgttta gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg
    22801 aagcggtggc aattggtcat atgaccgctg gcggtacagt agatgaacca atcagccgcc
    22861 acccgtccaa acgtactcac atggcggtgc atccgatggg caaaccggcg gtgactcact
    22921 atcgcatcat ggaacacttc cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac
    22981 gtacgcacca gatccgcgtg catatggccc atatcactca tccgctggtg ggcgatccgg
    23041 tttatggtgg ccgtccgcgt ccgccaaaag gtgcttcgga agcatttatc tccacgctgc
    23101 gtaagtttga ccgccaggcg ctacatgcaa ccatgctgcg tctttatcac ccgatctccg
    23161 gcatcgaaat ggaatggcat gcgcctattc cacaagatat ggtggagctg attgaggtga
    23221 tgcgcgccga tttcgaagaa cataaggatg aagtggactg gttatgagta agctgattgt
    23281 cccgcagtgg ccgctgccaa agggtgttgc ggcttgtagc tctactcgta tcggcggcgt
    23341 gagcttgccc ccgtatgact cgctcaacct cggtgcccat tgtggcgata acccggatca
    23401 cgttgtggag aatcgcaagc gactttttgc tgcgggcaat ttgccttcta aaccggtctg
    23461 gcttgagcag gtacacggca aagatgtgct taagcttact ggcgaacctt atgcctcaaa
    23521 acgagcagat gcctcttata gcaatacgcc tggcacggtt tgcgcagtga tgactgccga
    23581 ctgcctccct gtgctgtttt gcaatcgagc gggaacggaa gtcgccgccg ctcatgctgg
    23641 ctggcgtgga ctgtgcgcag gcgtgctgga agagacagtt tcctgttttg ctgataatcc
    23701 ggaaaatatt ctcgcctggt tagggccggc aattggtcca cgcgcgttcg aagtgggggt
    23761 ggaggttcgc gaggcgttta tggcagtaga cgctaaagca agtgcagctt tcattcagca
    23821 tggtgataag tatcaggcgg atatttatca gcttgcccgg cagcgtctgg cgaacgtggg
    23881 tgttgagcaa attttcggcg gcgaccgttg tacatatacg gaaaatgaga ctttcttctc
    23941 ttatcgtcgc gacaagacca ccggtcgtat ggcaagtttc atttggctga tataacctaa
    24001 agaatcaaga cgatccggta cgcgtgattt tcttttcaca ttaatctggt caataacctt
    24061 gaataattga gggatgacct catttaatct ccagtagcaa ctttgatccg ttatgggagg
    24121 agttatgcgt ctggatcgtc ttactaataa attccagctt gctcttgccg atgcccaatc
    24181 acttgcactc gggcacgaca accaattcat cgaaccactt catttaatga gcgccctgct
    24241 gaatcaggaa gggggttcgg ttagtccttt attaacatcc gctggcataa atgctggcca
    24301 gttgcgcaca gatatcaatc aggcattaaa tcgtttaccg caggttgaag gtactggtgg
    24361 tgatgtccag ccatcacagg atctggtgcg cgttcttaat ctttgcgaca agctggcgca
    24421 aaaacgtggt gataacttta tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg
    24481 cggcacgctg gccgacatcc tgaaagcagc aggggcgacc accgccaaca ttactcaagc
    24541 gattgaacaa atgcgtggag gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca
    24601 ggctttgaaa aaatatacca tcgaccttac cgaacgagcc gaacagggca aactcgatcc
    24661 ggtgattggt cgtgatgaag aaattcgccg taccattcag gtgctgcaac gtcgtactaa
    24721 aaataacccg gtactgattg gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct
    24781 ggcgcagcgt attatcaacg gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc
    24841 gctggatatg ggcgcgctgg tggctggggc gaaatatcgc ggtgagtttg aagaacgttt
    24901 aaaaggcgtg cttaacgatc ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga
    24961 attacatacc atggtcggcg cgggtaaagc cgatggcgca atggacgccg gaaacatgct
    25021 gaaaccggcg ctggcgcgtg gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata
    25081 tcgccagtac attgaaaaag atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc
    25141 cgaaccttct gttgaagata ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt
    25201 gcaccaccat gtgcaaatta ctgacccggc aattgttgca gcggcgacgt tgtctcatcg
    25261 ctacattgct gaccgtcagc tgccggataa agccatcgac ctgatcgatg aagcagcatc
    25321 cagcattcgt atgcagattg actcaaaacc agaagaactc gaccgactcg atcgtcgtat
    25381 catccagctc aaactggaac aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa
    25441 acgcctggat atgctcaacg aagaactgag cgacaaagaa cgtcagtact ccgagttaga
    25501 agaagagtgg aaagcagaga aggcatcgct ttctggtacg cagaccatta aagcggaact
    25561 ggaacaggcg aaaatcgcta ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc
    25621 tgaactgcaa tacggcaaaa tcccggaact ggaaaagcaa ctggaagccg caacgcagct
    25681 cgaaggcaaa actatgcgtc tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt
    25741 gctggcgcgt tggacgggga ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact
    25801 gctgcgtatg gagcaagaac tgcaccatcg cgtcattggt cagaacgaag cggttgatgc
    25861 ggtatctaac gctattcgcc gtagccgtgc ggggttggcg gatccaaatc gcccgattgg
    25921 ttcattcctg ttcctcggcc caacgggtgt ggggaaaact gagctttgta aggcgctggc
    25981 gaactttatg tttgatagcg acgaggcgat ggtccgtatc gatatgtccg agtttatgga
    26041 gaaacactcg gtgtctcgtc tggttggtgc gcctccggga tatgtcggtt atgaagaagg
    26101 tggctacctg accgaagctg tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt
    26161 ggaaaaagcg catccggatg tcttcaacat tctgttgcag gtactggatg atggtcgtct
    26221 gactgacggg caggggagaa cggtcgactt ccgtaatacg gttgtcatta tgacctctaa
    26281 cctcggttcc gatttgattc aggaacgctt cggtgaactg gattatgcgc acatgaaaga
    26341 gctggtgctc ggtgtggtaa gccataactt ccgtccggaa ttcattaacc gtattgatga
    26401 agtggtggtc ttccatccgc tgggtgaaca gcacattgcc tcgattgcgc agattcagtt
    26461 gaaacgtctg tacaaacgtc tggaagaacg tggttatgaa atccacattt ctgacgaggc
    26521 gctgaaactg ctgagcgaga acggttacga tccagtctat ggtgcacgtc ctttgaaacg
    26581 tgctatccag cagcagatcg aaaacccgct ggcacagcaa atactgtctg gtgaattggt
    26641 tccgggtaaa gtgattcgcc tggaagttaa tgaagaccgg attgtcgccg tccagtaaat
    26701 gataaaacga gcccttcggg gctcgttttt gtctataagt tagacggaaa agactatatt
    26761 taagatgttt tgcctgaaaa atgagcgaac gataaagttt ttatattttt c
//
LOCUS       DAOFQD010000064        26645 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.064, whole genome shotgun sequence.
ACCESSION   DAOFQD010000064 DAOFQD010000000
VERSION     DAOFQD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.064"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            6..677
                     /gene="mngR"
                     /locus_tag="QBF46_002704"
     CDS             6..677
                     /gene="mngR"
                     /locus_tag="QBF46_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDQ6674867.1"
                     /translation="MARGELKPGDALPTESALQTEFGVSRVTVRQALRQLVEQQILES
                     IQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIFEVIPADDFLQQQLQITP
                     QDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENSKYHFIEEVKKMVIDRSE
                     QEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEYSRNAFNTDDYKFTLIAQ
                     RKSSR"
     gene            complement(781..1650)
                     /gene="sucD"
                     /locus_tag="QBF46_002705"
     CDS             complement(781..1650)
                     /gene="sucD"
                     /locus_tag="QBF46_002705"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDQ6674868.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(1650..2816)
                     /gene="sucC"
                     /locus_tag="QBF46_002706"
     CDS             complement(1650..2816)
                     /gene="sucC"
                     /locus_tag="QBF46_002706"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDQ6674869.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKIALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(2910..4127)
                     /gene="odhB"
                     /locus_tag="QBF46_002707"
     CDS             complement(2910..4127)
                     /gene="odhB"
                     /locus_tag="QBF46_002707"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDQ6674870.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(4142..6943)
                     /gene="sucA"
                     /locus_tag="QBF46_002708"
     CDS             complement(4142..6943)
                     /gene="sucA"
                     /locus_tag="QBF46_002708"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDQ6674871.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(7244..7960)
                     /gene="sdhB"
                     /locus_tag="QBF46_002709"
     CDS             complement(7244..7960)
                     /gene="sdhB"
                     /locus_tag="QBF46_002709"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDQ6674872.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNEQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(7976..9742)
                     /gene="sdhA"
                     /locus_tag="QBF46_002710"
     CDS             complement(7976..9742)
                     /gene="sdhA"
                     /locus_tag="QBF46_002710"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDQ6674873.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHVKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(9742..10089)
                     /gene="sdhD"
                     /locus_tag="QBF46_002711"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(9742..10089)
                     /gene="sdhD"
                     /locus_tag="QBF46_002711"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDQ6674874.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(10083..10487)
                     /gene="sdhC"
                     /locus_tag="QBF46_002712"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(10083..10487)
                     /gene="sdhC"
                     /locus_tag="QBF46_002712"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDQ6674875.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            10868..10975
                     /locus_tag="QBF46_002713"
     CDS             10868..10975
                     /locus_tag="QBF46_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674876.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            11181..12464
                     /gene="gltA"
                     /locus_tag="QBF46_002714"
     CDS             11181..12464
                     /gene="gltA"
                     /locus_tag="QBF46_002714"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDQ6674877.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            12853..13419
                     /locus_tag="QBF46_002715"
     CDS             12853..13419
                     /locus_tag="QBF46_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6674878.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            13470..15928
                     /locus_tag="QBF46_002716"
                     /pseudo
     CDS             13470..15928
                     /locus_tag="QBF46_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            15943..16674
                     /locus_tag="QBF46_002717"
     CDS             15943..16674
                     /locus_tag="QBF46_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDQ6674879.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            16671..17732
                     /locus_tag="QBF46_002718"
     CDS             16671..17732
                     /locus_tag="QBF46_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6674880.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            17884..18930
                     /locus_tag="QBF46_002719"
     CDS             17884..18930
                     /locus_tag="QBF46_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDQ6674881.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(18927..19718)
                     /gene="nei"
                     /locus_tag="QBF46_002720"
     CDS             complement(18927..19718)
                     /gene="nei"
                     /locus_tag="QBF46_002720"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDQ6674882.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(19754..20488)
                     /gene="pxpA"
                     /locus_tag="QBF46_002721"
     CDS             complement(19754..20488)
                     /gene="pxpA"
                     /locus_tag="QBF46_002721"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDQ6674883.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(20478..21410)
                     /gene="pxpC"
                     /locus_tag="QBF46_002722"
     CDS             complement(20478..21410)
                     /gene="pxpC"
                     /locus_tag="QBF46_002722"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDQ6674884.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(21404..22060)
                     /gene="pxpB"
                     /locus_tag="QBF46_002723"
     CDS             complement(21404..22060)
                     /gene="pxpB"
                     /locus_tag="QBF46_002723"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDQ6674885.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVL
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(22083..22826)
                     /gene="ybgI"
                     /locus_tag="QBF46_002724"
     CDS             complement(22083..22826)
                     /gene="ybgI"
                     /locus_tag="QBF46_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDQ6674886.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            23097..24578
                     /gene="dtpD"
                     /locus_tag="QBF46_002725"
     CDS             23097..24578
                     /gene="dtpD"
                     /locus_tag="QBF46_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDQ6674887.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(24728..26146)
                     /gene="phrB"
                     /locus_tag="QBF46_002726"
     CDS             complement(24728..26146)
                     /gene="phrB"
                     /locus_tag="QBF46_002726"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDQ6674888.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDHEGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(26143..>26645)
                     /locus_tag="QBF46_002727"
     CDS             complement(26143..>26645)
                     /locus_tag="QBF46_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDQ6674889.1"
                     /translation="QRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVFLAH
                     SQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGYFRP
                     HIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRVINL
                     RHSGVL"
BASE COUNT         6323 a         7201 c         6639 g         6482 t
ORIGIN      
        1 aacaaattgc gcgtggcgag cttaaacctg gcgatgcgtt acccactgaa tcagcgctac
       61 aaacagagtt tggcgtcagc cgggttacgg tgcgtcaggc tttgcgccag ctagtcgagc
      121 agcagatcct cgaaagcatt cagggcagcg ggacttacgt caaagaagag cgggtcaatt
      181 acgatatttt tcagttaacc agttttgatg aaaaactgtc ggaccgtcac gtcgatacac
      241 acagtgaagt tctgatattc gaagtgattc cggctgacga ttttcttcag caacagctac
      301 aaatcacccc gcaggatcgt gtgtggcatg tgaagcgcgt gcgctatcgc aagcaaaagc
      361 caatggcgct ggaagaaacc tggatgccgc ttgcgttgtt cccggatctc acctggcagg
      421 tcatggaaaa ttcgaaatat cactttatcg aagaagtgaa gaaaatggtt atcgatcgta
      481 gcgaacagga aatcattcct ctgatgccaa ccgaagagat gagccgtctg cttaatatca
      541 gccagacaaa gccgattctt gaaaaagtat cgcgtggata tttagttgat ggtcgggtgt
      601 ttgaatatag ccgcaacgct tttaataccg atgactataa atttaccctg atagcgcagc
      661 gaaaatcatc gcgataagca caaaaaaggc catcatacga tggccaacca tgtcgaaacc
      721 gacagccctt cgccattcct gtagagggaa gcggcgaggg ctatttctta ttacagatat
      781 ttatttcaga acagttttca gtgcttcacc gatatccgcc aggctgcgaa cggttttcac
      841 gcctgcggct tccagagcag cgaatttctc atccgcagtc cctttcccac cggcaatgat
      901 ggcacccgcg tggcccatac gtttgccttt cggcgcagtc acaccagcga tgtaacccac
      961 aactggcttg gtaacgtgct ctttgatgta cgcagctgct tcttcttcag cgctaccgcc
     1021 gatctcaccg atcatcacga tcgcttcggt ctgcggatct ttttcgaaca tttcgagaat
     1081 gtcgataaag ttagagcccg ggatcgggtc accgccgata ccaacacagg tcgactgacc
     1141 gaaaccgtaa tccgtggtct gtttaaccgc ttcataggtc agtgtaccgg aacgggaaac
     1201 gatacccact ttacccggtt tgtgaatgtg acccggctgg ataccgattt tgcattcacc
     1261 cggagtgata acgcctgggc agttcgggcc gatcatacga acgccggctt cgtccagttt
     1321 cactttcaca gtcagcatat ccagcgtcgg gatgccttca gtgatggtga taatcagttt
     1381 gatgcctgcg tcgatggctt ccagaatgga gtctttgcag aacggtgctg gtacgtagat
     1441 aacagaagcg gtagcgccag tggcagcaac ggcttcacgc acggtgttga acaccggcag
     1501 gccgaggtgg gtggtgccgc ctttacctgg ggttacgccg ccaaccattt tagtgccgta
     1561 tgcaatggcc tgttctgagt ggaaagtccc ctggctaccg gtaaagccct ggcagataac
     1621 cttggtgttt ttatcgatta aaatggacat tatttcccct ccactgcggc aacaacctgc
     1681 tgagctgcat ccgtcagacc ttttgctgca ataatattca ggccgctgtc agccagtttc
     1741 ttcgcgccga gttcggcgtt gttaccttcc agacgtacca cgaccggtac gttaacaccc
     1801 acttctgcta ccgcgccgat gataccgtca gcgatcaggt cgcaacgaac gataccgccg
     1861 aagatgttaa ccagaacggc tttcactttg tcgtcagaga ggatgatttt gaacgcttcg
     1921 gttacacgtt ctttggttgc gccgccgcca acgtcaagga agttagccgg ttcgccgccg
     1981 tgcagtttaa cgatgtccat cgtacccatc gccagacctg cgccgttaac catacaaccg
     2041 atgttaccgt ccagcgcaac gtagttcagt tcccactgtg cagcctgtgc ttcacgcgga
     2101 tcttcctgcg actggtcacg catttcgcgc agatcaggct ggcggaacag tgcgttgccg
     2161 tcagcgccca gtttgccgtc gaggcaaatc agatcgccct gtttggtgat gaccagcggg
     2221 ttgatttcga tcaacgccag gtcgcgctcc aggaaaatgg tcgccaggcc catgaagatt
     2281 ttggtgaact gctgaaccag tttaccttcc agacccagtt tgaacgccag ctcgcgtccc
     2341 tgatacggca tcgggccagt cagcggatca agcgcaattt tgtggatcag gtgcggagtt
     2401 tcttccgcca ctttttcgat ttccacgccg ccttcggtgg aggccataaa gaccacacga
     2461 cgggaactac ggtctacaac ggcaccgaga tacagctctt tagcgatatc ggtcgctgct
     2521 tcaaccagaa tctggttaac cggttggcca ttggcatctg tttgatacgt taccagacgc
     2581 ttgccgagcc agttttctgc aaaagcacgg atgtcttctt tgctgtttac aactttcaca
     2641 ccgcccgctt taccgcggcc accagcgtga acctgacatt tcactaccca cgggccggca
     2701 ccgatttttg aagcggcttc ttctgcttcg cgcggagtag tacaggcata acccaccggt
     2761 gctggtaagc catagcgggc aaaaagttgt tttgcctgat attcatgtaa gttcatgtgt
     2821 tctgtccatc cttcagtaat cgttatcttt taaaccgtag accgacaccc tatccacgtc
     2881 ggtctacagt gcaggtgaaa cttaaactac tacacgtcca gcagcagacg cgtcggatct
     2941 tccagcaact ctttgatcgt taccaggaag cccacggatt cgcgaccatc gatcagacgg
     3001 tgatcgtagg acagcgccag gtacatcatc ggcaggatct caacctgacc attcaccgcc
     3061 atcggacgat ctttgatagc gtgcataccc agaattgcgc tctgcggcgg gttgatgatc
     3121 ggcgtagaca tcagggaacc gaacacacca ccgttggtga tggtgaagtt accaccggtc
     3181 agatcttcaa cggtcagctt gccgtcacgg cctttgactg ccagctcttt gattttcttc
     3241 tcgatgtctg ccatgccgag ggtatcgaca tcacgcagta ccggtgtcac caggccgcgc
     3301 ggcgtagaaa ccgccatgct gacgtcgaaa tagttgtggt aaaccacgtc atcgccgtcg
     3361 atagacgcgt tcacttccgg gtaacgtttc agggcttcaa ccaccgcttt cacgtagaag
     3421 gacataaagc ccagacggat gccgtggcgt ttttcaaacg cttcaccgta ctgcttacgc
     3481 agatccataa tcggcttcat gttgacttcg ttgaacgtgg tcagcatggc ggtggagttt
     3541 ttcgcttcca gcagacgctc tgccacacgc ttacgcaggc gagtcatcgg gacacgtttt
     3601 tcactacgtg cagccagagc cggttgcgcc gccggagcag ccgctgccgg agcagactct
     3661 ttcgccgggg ctttcgccag atgtttttcc acatcttcac gagtcagacg accacccaca
     3721 ccggtgcctt taatggcgct ggcgtcgaga ttgtgttcag ccagcaggcg acggatcgcc
     3781 gggcttaacg catcgttgtt ttgctcttcc agagacgcct gctggcgttg cgccggagtg
     3841 gacgctttct cttcagattt ggcgctggtt tctttaccgg cgctgttgcc ttcacgcagg
     3901 cgaccaagga tctgacgaga cgttacggtt gtaccttcat cttccagaac cgcatccaga
     3961 atgccgtctg ctgatgccgg tacttccagt accactttgt cagtttcgat ttctaccagc
     4021 acttcatcac gtacgactgc gtcgccgggt tttttatgcc aggttgcgac ggtggcatcg
     4081 gctacggatt caggcaggtc agggaccaga atatctacgc tactcattgt gtatccttta
     4141 tttattcgac gttcagcgcg tcattaacca gatcttgttg ctgtttctgg tgaacggaca
     4201 tataccctac cgccggagag gcggaggccg ggcggcctgc ataacgcaga gaagccccaa
     4261 acggaatcac ttcacggaaa tgatgctggc tgcagtacca tgcgccctgg ttgagcggct
     4321 cttcctggca ccagacaaaa tccttgacgt gagcaaactg ctgcaacact tcctgcatcg
     4381 ctttatgcgg gaacgggtag agttgctcga tacgcacaat ggcgacatcg tgttgattgt
     4441 tcttacgacg ctgttccagc aggtcgtaat aaaccttacc agaacacatt actacgcgct
     4501 tcacgccctt cggatcaagc tcgtcgattt caccgatggc tggcaggaag gtgccgttcg
     4561 ccagttcttc gaggctggaa accgccagcg gatgacgcag cagggatttc ggcgacatca
     4621 cgaccagcgg acgacgcatc ccgcgcagcg cctgacgacg cagcatgtgg taaacctgtg
     4681 ccggggtaga cggtacgcaa acctgcatgt tttgctcagc acaaagttgc agataacgtt
     4741 ccagacgcgc ggaggagtgc tccggcccct gcccttcgta accgtgcggc agcaacatca
     4801 ccaggccaca catccgaccc catttctgtt cgccagaaga gatgaactgg tcgataacca
     4861 cctgcgcacc gttggcgaag tcaccgaact gcgcttccca gatagtcaga gtgcgtggtt
     4921 ctgcggtggc ataaccgtat tcaaacgcca gcactgcttc ttcagacagt acggagtccc
     4981 agacgcggaa cgcgccctgc ccgttatgga tgtgttgcaa cggcgtgtaa gtggaaccgt
     5041 tagactggtt gtggatcacc gcgtggcggt ggaagaaggt accgcgaccg gagtcttcac
     5101 ccgacaggcg aaccggaatg ccttcgtcaa ccagcgtggc gtaagcgagg ttttccgcac
     5161 cgccccagtc gaacagtttc tcaccggcag ccatcgcctg gcgatcgcca taaatcttgg
     5221 caacgcgaga ctgcatttca actgcttccg gcaccgtgct gatgcgtttc gccagctcct
     5281 gcaggcgctt catctcaact ttgttcgggt actcttcgtc ccattcgtgg ttgaggtacg
     5341 gcgaccaggt gaaagagtgc atgttcatcg gacgccactc tgccactacg caatcgccag
     5401 catccagcgc atcgcggtac aggttaacca tctcggtggc atcttccagc gtcgccactt
     5461 tttcctgctc cagcttgtca gcgtagattt tgcgcggtgt cggatgtttt ttgattttct
     5521 gatacatcag cggctgggtt gcgctcggct cgtcggcttc gttgtggccg tgacggcggt
     5581 agcacaccag gtcgatgaag acatcacgtt taaaggtgtt acggaaatcg agcgccagac
     5641 gggtcacaaa ggcaacggct tccggatcat ccgcgttaac gtggaaaatc ggtgcctgaa
     5701 ccatcttacc gatatcagta cagtacggtg tagaacgagc atccagcgga ttagaggtgg
     5761 tgaaaccaac ctggttgttg ataacgatac gtaccgtacc gccaacttca taaccacgcg
     5821 ctttcgacat gttcagagtt tcctgaacca cgccctgccc ggtcactgcg gcgtcaccgt
     5881 ggatggtgat tggcagcact ttgttgctgc tcggctcatc aagtctgtcc agacgggcac
     5941 gaacagaacc gataactacc gggcttacaa tctcaaggtg agacgggtta aacgccagcg
     6001 ccaggtgcac caggccgcca tcggtctgga agtcagacga gaagcccatg tggtatttca
     6061 cgtcacccgt gccgaggtgt tctttatgtt taccggcaaa ctcgtcgaac aagtcttgcg
     6121 gttttttacc cagcacgttc accagcacgt tcagacgacc acggtgcgcc atcccgagaa
     6181 ccacttcccg ggtgccgctg ttgccagcgt ggcggatcat ctctttaagc atcgggatta
     6241 acgcgtcacc gccttccagc gagaagcgtt ttgcaccagg gaattttgca ccgaggtaac
     6301 gttcaaggcc ttcagcggcg gtcagttcgc ttaagaagcg ttttttctct tcactattga
     6361 aagtcgcgcg accagactcg atacgctgtt ggatccagcg tttttcttcg gtgctggtga
     6421 tgtgcatata ctcggcacca atcgggccgc agtaggtttg cttgagggct tccagcagct
     6481 cgccgagttt catggtttct ttgccgctgg caaatgaacc gacgttgaag gtctcctgga
     6541 agtctgcttc ggtcagatcg tggaaagacg gatccagatc ggccacttta tcttgctgcc
     6601 acagtcccag cggatcgaga ttcgcatgct ggtgaccacg gaagcggtat gcgttaatga
     6661 gctgcaggac ttttacctgc ttcacattgg tgtcagggtc ggagatcgtt gaagagtaac
     6721 gtgaagcgtc tttcgccagg cggcggaaat attcacgcgt ttgagagtgg aattgatccg
     6781 gtttgactcc cgtaccaggt aactgctgga acgtcgaacg ccagttagcg tcaaccgagt
     6841 caggatcggt taagaagtct tcatagagct gttctatcca gctctggttt gcgccagaga
     6901 ggtaagaaga gtccaaccag gctttcaaag cgctgttctg catcgtgatc ccttaagcat
     6961 cttttttatg cttacttcgc cgtggatact accacgcaca gtgatgtgcg tgccggggtt
     7021 cacttgcgag ctcttgtcta cgtagtggct cgcgaaggaa cctttaaaaa ctgtcttagt
     7081 gtcaggggca gtttttaaag atttcctgca tttctttgtt acgcctgatg cgctgcgctt
     7141 atcaggccta cgggtaatga cttgtaggcc ggataaggcg ttcacgccgc atccggcact
     7201 ggttgcctga tgcgacgctt gcgcgtctta tcaggcctac ggtttacgca ttacgttgca
     7261 acaacatcga cttgatatgg ccgatggcgc gcgtcgggtt cagccccttc ggacatacac
     7321 tgacgcagtt catgatgctg tgacagcgga atacgctgaa tgcatcactc aaaccgtcga
     7381 ggcggctgtc agtctcggta tcacggctat cgatcaggaa acgatatgcc gctaacaagc
     7441 ctgccgggcc gataaattta tcgggattcc accagaaaga cggacaagag gttgaacaac
     7501 atgcgcagag aatacattca tacagcccgt cgagtttttc gcgctgctct ggcatctgta
     7561 aatgctcgcg agctggcgga ttttgttcat tattcaacag gtaaggctta attttctcat
     7621 attgcgcata gaattgtccc atgtctacca ccaaatcgcg gatcaccggt aaacctggca
     7681 gagggcgaat cacaatcttc ttgcccggct ggttgagtgc cgaaatcggg gtaatacagg
     7741 ccagaccatt cttgccgttc atgttcagac cgtcggaacc gcacacacct tcacgacagg
     7801 agcgacggaa cgacaggctg ggatctttct ctttcagctg gataagcgca tccagcagca
     7861 tcatgtcgcg accttcttcc gcttccaggg tgtaatcctg catacgcgga gcatcatcaa
     7921 catccgggtt atagcgataa attgaaaact cgagtctcat tttcctgtct ccgcattagt
     7981 aagtacgaat cttcggcggg aatgccgggc gcagtttcgg ttccatgttg acgcttcggc
     8041 gcgtcatgga ttccgactct ggcagataca gggagtggca cagccagttt tcatcatcac
     8101 gatccgggaa gtcgaagcgg ctatgcgcgc cacggctttc ggtacggaag ttggcagaaa
     8161 cagccgttgc atacgccgtt tccatcaggt tatccagttc cagacactca acgcgctggg
     8221 tgttgaactc gctggaagtg tcatccagac gggcattttt cagacgctcg cggataactt
     8281 tcaactgctc aagccctttc gccatcgcat caccttcacg gaagaccgag aagttatgct
     8341 gcatacattc ttgcagcgct ttacgaatcg ccaccggatc ttcaccgtta cgattattgt
     8401 tccagcggtt cagacgatcc agagatgctt caacatcaga ctcgctggca tcgcgcagtg
     8461 cgccctgctc ggcgatagac tcttgcagat gcagacccgc tgcgcgacca aagaccacca
     8521 ggtccagcag cgagttaccg cccagacggt tagcaccgtg taccgatacg caggcaattt
     8581 cacctaccgc gaacagcccc ggaacaacca catcttcgcc tttctcattc acagtcagcg
     8641 cctgaccggt cactttggtc ggaataccgc ccatcatgta gtgacaggtt gggataaccg
     8701 gaatcggctc tttcaccgga tcaacgtgag cgaaggtacg ggaaagctcc aggatacccg
     8761 gcagacggga ttcgagaact tctttaccca ggtgatcgag tttcagtttc acgtgtggcc
     8821 cccacggacc atcacagccg cgaccttcac ggatttcaat catgatggaa cgcgccacca
     8881 catcacggcc cgccaggtct ttggcgttcg gcgcataacg ctccataaag cgttcgccat
     8941 gtttgttcag cagataaccg ccttcaccac ggcaaccttc ggtgaccagt acgcccgcac
     9001 cggcaatgcc ggtcgggtgg aattgccaca tttccatatc ctgcaccggt acgcctgcgc
     9061 ggatagccat gccgacaccg tcgccagtgt taatgtgggc gttagtggtg gactgataaa
     9121 tacgccctgc cccgccagtc gccagcacgg tagcgcgggc tttgaaataa accacttcac
     9181 cggtttcgat gcacagtgcg gtacaaccca ccaccgcgcc atcctggttt ttcaccagat
     9241 ccagcgcata ccactcggag aaaatggtgg tgtggttttt caggttctgc tgataaagcg
     9301 tgtgcaacag tgcgtgaccg gtacggtcag ccgctgccgc agtgcgtgcc gcctgctcgc
     9361 cgccgaagtt tttcgactga ccgccaaacg gacgttgata gatacgacca tcatcaagac
     9421 gcgagaacgg caggcccatg tgttcgagtt ccagaatcgc ttccggcccg gttttacaca
     9481 tatattcaat cgcgtcctgg tcaccgatat agtccgaccc ttttacggtg tcgtacatat
     9541 gccattccca gttatcttca tgggtattac ccagcgcaac ggtaatgccg ccttgcgcag
     9601 aaacggtatg ggaacgggtc gggaagactt tagagagcag cgcacaggtc tggccgctct
     9661 gggaaatttg cagcgccgcg cgcatacctg cgccgccggc accaatcaca actgcatcaa
     9721 attctctgac tggcaatttc atcacacacc ccacaccaca acgaatccat aaatcacgta
     9781 aaccaccagt gcaacgacaa tcaccagttg cagcatcagg cgcaaagcca gcggtttaac
     9841 gtagtcggtc aacacctgcc acatgccgat ccaggcatgg atcaagatag aaaacagcgc
     9901 cagcagggtg aacactttgg tgaacgcaga ggcgaagaaa ccgatccaga cttcatatgt
     9961 cagctcgcca ctggtagcga aaaaaccgac catataaatg atgtagagcg tcaggacgat
    10021 agcggtagcg cgaacgagga tgaaatcatg tacgccattg cgtcctaatg cggaggcgtt
    10081 gcttaccata cgaggactcc tgcgagaagt gaaagcacga cagtaataac aaaggagatt
    10141 ttggcggagc gtttacccgc ttcgaatgtt tcttccagat agccaaaatc catcatcatg
    10201 tggcgaatac ctacgacgac gtgatacgcc agagcggtaa ggatgcccca catgataaat
    10261 ttgacgaaga agctgcccat aatcgcggaa gcttgctcga aaccttcagg ggaagagagg
    10321 ctggtaccca gaagccacag caggatgccc actgcaacaa aggtgatcac accggaaacg
    10381 cgatggagaa tggacgctat cgccgtgatg gggaaccgga tggtctgtag gtccagatta
    10441 acaggtcttt gttttttcac atttcttatc atgaataacg cccacatgct gttcttatta
    10501 ttccctgggg actacgggca cagaggttaa ctttctgtta cctggagacg tcgggatttc
    10561 cttcctccgg tctgcttgcg ggtcagacag cgtcctttct ataactgcgc gtcatgcaaa
    10621 acactgcttc cagatgcgaa aacgacacgt tacaacgctg ggtggctcgg gattgcaggg
    10681 tgttccggag acctggcggc agtataggcc gttcacaaaa tcattacaat taacctacat
    10741 atagtttgtc gggttttatc ctgaacagtg atctaggtca cgataacaac atttatttaa
    10801 tttttaatca tctaatttga caatcattca acaaagttgt tacaaacatt accaggaaaa
    10861 gcatataatg cgtaaaagtt atgaagtcgg tatttcacct aagattaact tatgtaacag
    10921 tgtggaagta ttgaccaatt cattcgggac agttattagt ggtagacaag tttaataatt
    10981 cggattgcta agtacttgat tcgccattta ttcgtcatca atggatcctt tacctgcaag
    11041 cgcccagagc tctgtaccca ggttttcccc tctttcacag agcggcgagc caaataacaa
    11101 acgggtaaag ccaggttgat gtgcgaaggc aaatttaagt tccggcagtc ttacgcaata
    11161 aggcgctaag gagaccttaa atggctgata caaaagcaaa actcaccctc aacggggaca
    11221 cagctgttga actggatgtg ctgaaaggca cgctgggtca agatgttatt gatatccgta
    11281 ctctcggttc aaaaggtgtg ttcacctttg acccaggctt cacttcaacc gcatcctgcg
    11341 aatctaaaat tacttttatt gatggtgatg aaggtatttt gctgcaccgc ggtttcccga
    11401 tcgatcagct ggcgaccgat tctaactacc tggaagtttg ttacatcctg ctgaatggtg
    11461 aaaaaccgac tcaggaacag tatgacgaat ttaaaactac ggtgacccgt cataccatga
    11521 tccacgagca gattacccgt ctgttccatg ctttccgtcg cgactcgcat ccaatggcag
    11581 tcatgtgtgg tattaccggc gcgctggcgg cgttctatca cgactcgctg gatgttaaca
    11641 atcctcgtca ccgtgaaatt gccgcgttcc gcctgctgtc gaaaatgccg accatggccg
    11701 cgatgtgtta caagtattcc attggtcagc catttgttta tccgcgcaac gatctctcct
    11761 atgccggtaa cttcctgaat atgatgttct ccacgccgtg cgaaccgtat gaagttaatc
    11821 cgattctgga acgtgctatg gaccgtattc tgatcctgca cgctgaccat gaacagaacg
    11881 cctccacctc caccgtgcgt accgccggct cttcgggtgc gaacccgttt gcctgtatcg
    11941 cagcaggtat tgcttcactg tggggacctg cgcacggtgg tgctaacgaa gcggcgctga
    12001 aaatgctgga agaaattagc tccgttaaac acattccgga atttgttcgg cgtgcgaaag
    12061 ataaaaatga ttctttccgc ctgatgggct tcggtcaccg cgtgtacaaa aattacgacc
    12121 cgcgcgccac cgtaatgcgt gaaacctgcc atgaagttct gaaagagctg ggcaccaaag
    12181 atgacctgct ggaagtggct atggagctgg aaaacatcgc gctgaacgac ccgtacttta
    12241 tcgagaagaa actgtacccg aacgtcgatt tctactctgg tatcatcctg aaagcgatgg
    12301 gtattccgtc ttccatgttc accgtcattt tcgcaatggc acgtaccgtt ggctggatcg
    12361 cccactggag cgaaatgcac agtgacggta tgaagattgc ccgtccgcgt cagctgtata
    12421 caggatatga aaaacgcgac tttaaaagcg atatcaagcg ttaatgattg attgctaagc
    12481 cgtttacttt ccggacccgc ctttaataga gcgggttctt tttatatggc tgaacataat
    12541 aaattgtaaa aattaaacca gatacaaata cccattaata aggtgagaaa atgtgacaag
    12601 tcacatactg agcagaatga taagtttatt tttatacttt cctgctggta gcattgttaa
    12661 cccaatgaaa attatgcata tccatcccac cccatccttc atctcattaa acaataaatt
    12721 tatgcactca attattccat tctttatata acaagatttt taatattgtt aaataaaacc
    12781 aaatacattc aactgccagt taatatattc ttgccacgca acattctatc tcattattta
    12841 aaggactccg ttatgtttaa aggacaaaaa acattggccg cactggccgt atctctgctg
    12901 ttcacagcac ctgtttatgc ggctgatgaa ggatccggtg aaattcactt taaaggtgaa
    12961 gttattgaag caccgtgtga aatacatcag gatgatattg ataaagaggt tgaactcggt
    13021 caggtgacca ccagccacat taatcagtca catcacagcg atgccgttgc tgtcgacctg
    13081 cgcttagtca actgtgatct ggaaaactcc agcaacggtt ccggtggcaa gatttctaaa
    13141 gttgcagtca cctttgatag ctcagcgaaa accaccggcg cagatccgat tctcaacaac
    13201 accagcacgg gtgaagccac cggcgtcggc gtacgtttaa tgaataaaga tcaaagcaat
    13261 atcgttttag gcaccgctac tccagatatc gacctggctc cgacctccag cgaacaaacg
    13321 ctgaatttct ttgcctggat ggaacaaatt gatcaggcta cacctgtaac gccaggcgca
    13381 gttacggcga atgcaaccta cgtgctggat tataaataag tattactttg gcggcgctat
    13441 cgccgccttt cataaaatat ttatcaggta tggacaccgt gaatatttat cgactctctt
    13501 ttgtatcctg cctggtcgtg gcgatgcctt gcgcattggc ggtcgaattc aaccttaatg
    13561 ttctcgataa atcgatgcgc gaccgcattg atatttcatt attaaaggaa aaaggagtca
    13621 ttgctcccgg tgagtatttt gttagcgttg cggtaaataa taaccaaatc agtaacgggc
    13681 aaaagattaa ctggcacaaa aatgacgata aaaccattcc gtgcatcaat gatttactgg
    13741 tcgataaatt tggcttaaaa cctgaagtcc gtcagtcgtt accattgata aatcagtgcg
    13801 tcgattttag ctcccgacct gaaatgctct tcaatttcga tcaagccaat cagcaactga
    13861 atattagtat tccgcaagcc tggctggtgt ggcactcaga aaactgggct cccccctcta
    13921 catggaaaga aggtgttgcc ggtgtcctga tggattataa cctgtttgcc agcaactacc
    13981 gcccacagga cggcagcaac agcactaacc tgaacgccta cggtaccacc ggaattaacg
    14041 ccgggtcatg gcgcttacgc agtgattacc agcttaataa caccgatagc gaagatagcc
    14101 atgaacagtc aggcggaata tcgcgcacct atctttttcg tccattaccg caattaggct
    14161 ctaaattaac cctcggcgaa accgatttca gttccaatat tttcgatggt ttttcttata
    14221 ccggcgcggc actggcgagt gacgatcgaa tgttgccatg ggagctacgc ggctacgccc
    14281 cacaaattag cggtattgca cagaccaatg ccacggtgac gatcagtcaa tcaggccgcg
    14341 tcatttacca gaaaaaagtc ccaccaggcc catttatcat tgacgacctt aatcagtctg
    14401 ttcagggcac actggatgtc aaagtgacgg aagaagatgg tcgggtgaac aatttccagg
    14461 tttcggcagc atcgacgccc ttcctgactc gtcagggaca ggttcgctat aaactggccg
    14521 cgggtcagcc acggccctcc atgtcacatc aaactgaaaa tgaaaccttt tttagcaatg
    14581 aagtttcctg ggggatgctg tcaaacacct cgctgtacgg cggcctgctg ctttctggtg
    14641 atgactacca ttctgccgca atgggtattg ggcaaaatat gctgtggctt ggtgcgctgt
    14701 cgtttgatgt cacgtgggcc agtagccatt ttgatactca gcaggacgag cggggcttaa
    14761 gctaccgttt taattacagc aaacaagtgg atgccactaa cagcacgatt tccctcgccg
    14821 cttatcgttt ctccgatcgt cattttcaca gctacgccaa ctatctggat cacaaataca
    14881 acgacagcga tgcgcaggac gaaaaacaga cgatcagctt atctgtgggc caaccgatta
    14941 ccccactaaa cctcaatctt tacgccaacc tgctacatca aacctggtgg aatgcagacg
    15001 cctccacgac cgccaacatc acagccggtt ttaatgttga tattggtgac tggagagata
    15061 tctctatttc gacgtcattc aatacaaccc attacgaaga taaagatcgc gacaaccaga
    15121 tttacctgtc gatttcgctc cccttcggta atggtggtcg ggttggttat gacatgcaaa
    15181 acagtagcca cagcaccaca caccgcatgt cgtggaacga tacactggat gaacgtaata
    15241 gctggggcat gtctgccgga ctgcaatccg accgtccgga caatggagcc caggtgagcg
    15301 gtaactatca gcatctgagt tcagcgggtg agtgggatat tctggtacct atgccgccaa
    15361 tgattacagt tccgtcagca gcagctggag cggttctttc accgcaaccc aatatggtgc
    15421 agcgttccat cgccgcagct ccaccaatga accacgcctg atggtcagca ccgatggcgt
    15481 ggcagatatt ccggttcagg gcaatctcga ctacaccaac cattttggca ttgcggtggt
    15541 gccgttgatt tccagttacc agccttccac cgtggcggtg aacatgaatg acttacccga
    15601 cggcgtaaca gttgcagaaa acgttatcaa agaaacgtgg attgaaggcg cgataggtta
    15661 caaatcactg gcttcccgtt ccggtaaaga cgttaacgtc atcattcgca acgccagcgg
    15721 tcagtttcct cccctcggag cggatatccg ccaggatgac agcggcatta gcgtggggat
    15781 ggttggcgag gaaggacatg cctggttaag cggagtcgct gaaaatcaaa agtttaccgt
    15841 ggtctggggt gatagccagc attgctcgct ccatcttcct gaacatatgg aagacaccgc
    15901 aaatcgcctg attttacctt gtcattaatt aaggaaatga aaatgacatt tatgaaagga
    15961 ctaccccttt tgttgttggt tgccagtctg tgcagccacg ctgcactaca acccgatcgc
    16021 actcgtattg tgtttaacgc taatgataaa gctaccagtc tgcgggtgga taatcgtagc
    16081 gataaactcc cctacctcgc ttattcctgg ctcgaaaatg aaaaagggga gaaaagcgat
    16141 gatcttctgg tcgccctgcc gccgatacaa cgtctggaac cgaaagcaac aacgcaggta
    16201 cggatagtaa agcaagcctc gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc
    16261 tacaatatgc gtgaaattcc tcccgccccg gaaaaaaaca gcgaccatgc cgttctgcaa
    16321 gtcgccattc agagtcgtat caaggtgttc tggcgaccgg ctgcattacg caagaaagca
    16381 ggagaaaagg tcgaactgca gttacaggtc agccagcagg gcaaccaact gacactgaaa
    16441 aatcctaccg cgtattatct gaccattgcc tacctgggac gaaatgaaaa aggcgttctc
    16501 cccggcttca aaaccgtgat ggttgcgcct tttagtacgg tcaacacaaa caccggaaat
    16561 tatagcggca gccagtttta tctcggttat atggatgatt acggcgcgtt acgcatgacg
    16621 acgctcaact gcagcggaca atgccattta caagccgtgg aggcgaagaa atgagtgctg
    16681 gcaagggagt gttgctcgtc atctgtctgt tatttctgcc gttgaagtct gcgcttgccc
    16741 tgaactgcta ctttggttca tccggtggct cggtagaaaa atcagaagcg attcaacctt
    16801 ttgctgtacc aggcaatgcc aaactcggcg ataagatttg ggaatctgac gatattaaaa
    16861 ttcccgtcta ttgtgacaac aataccaacg gcaattttga aagtgagcat gtttatgcct
    16921 gggtaaaccc ctacccgggg gtgcaggatc gctattatca actcggcgta acttacaatg
    16981 gcgttgatta tgacgccaac cagggaaaaa gccgcatcga cactaaccag tgtattgaca
    17041 gtaagaacat tgacatttat acccctgagc agattatcgc gatgggatgg cagaacaaaa
    17101 tttgctcggg tgatcccgct aatattcaca tgtcacgtac ctttctcgct cgcatgcggt
    17161 tatacgtcaa aatacgagaa atgccgccgc atgattatca aagtacgctt agcgactata
    17221 tcgtcgtgca atttgacggt gccggtagcg ttaacgaaga ccctactgcc caaaacctga
    17281 aatatcatat tactggtctg gaaaacattc gcgtgctgga ttgcagcgtc aatttttcca
    17341 tttcaccgga aacacaggtg attgattttg gtaaatttaa tttgctggat attcgtcgcc
    17401 acacaatgtc gaaaacattc agtattaaga cgacaaaatc acaaaatgac caatgcaccg
    17461 acggatttaa agtcagttcc tccttttata ccgaagaaac gttggttgaa gaagataaag
    17521 cattgttgat tggtaatggt ctgaagctac gtttgctgga tgaaaacgcc tcgccttata
    17581 ccttcaataa atatgctgaa tacgccgatt tcaccagcga catgttggtc tacgaaaaaa
    17641 cctatacagc tgaactttcg tccattgcag gtacccccat cgaggctggg cccttcgata
    17701 cagtggtgct ttttaaaatt aactataact gatttgccat gatgcccttt gcagccggaa
    17761 agggcatctc tttcagaaaa ttgctgattt tccccttttc cttgattgct gttatttttt
    17821 cctggcccgg ctttctttat gctgtatcga tacaccaaag agaaaataat gagggagcga
    17881 aggatgccag ttttgcagtg gggaatgtta tgtgtgctat cacttctcct ttctattggt
    17941 ttcctcgcgg tacatctccc ggcggcgcta ttacttgggc cgatgatcgc cgggatcatc
    18001 ttcagtatgc gcggaattac tctgcaactt ccccgctccg cttttctcgc cgcgcaggcc
    18061 attcttggct gcatgattgc gcaaaacctc actggttcga ttctcaccac gctggccgcc
    18121 aactggccga tcgtgcttgc tattttactg gtgacgctgc tttccagcgc catcgtgggt
    18181 tggttattgg tgcgctatag ctcactgcct ggaaataccg gtgcctgggg ttcttctcca
    18241 ggcggcgcag cggcaatggt cgctatggcg caagattacg gcgcagatat tcgcctggtg
    18301 gcgtttatgc aatatctgcg agtgctgttc gtcgcggggg ccgctgttct ggtcacccgc
    18361 atgatgctgg gtgataacgc tgaagcggtt aaccagcaaa tcgtctggtt cccgccagtg
    18421 agcattaatc tcctgcttac gattttgctc gcagtcgttg ccggtacggc gggatgcatg
    18481 ctgcgtcttc cttccggcac gatgctcatc ccgatgctgg cgggcgcagt gctccagtct
    18541 ggtcagctca tcaccatcga actaccggaa tggctgctgg cgatggcgta tatggcaatt
    18601 ggctggcgga ttggtcttgg tttcgataaa caaatcttac tgcgggcact acgcccacta
    18661 ccgcaaatcc tgctgtcgat ttttgctctg ctggctattt gtgcaggtat ggcgtgggga
    18721 ctgacccggt ttatgcatat tgattttatg actgcctacc tcgccaccag tcccggcggg
    18781 cttgataccg tggcggtcat cgccgcaggg agcaatgccg atatggcgct tattatggcg
    18841 atgcaaaccc tgcgcctgtt cagtattttg ctgacggggc cagccattgc gcggtttatt
    18901 tcaacctatg cgccgaagcg gtcggcctag tgctggcagc ctggacacca gtaaaacggg
    18961 cgcgaggaca acgtggtttt ctcaatgatg ccgccacagc gttcgcacag ttcgccatca
    19021 cgatgaaaaa ccttaaagcg aaacagcgcc ccatgatgct tattctcatc cacctgcccc
    19081 cgcgtagcgt aagaaaaccg aggaatctcc agtaacgcgt gtgcgagtgc atccagttgc
    19141 gccgcattga gatctttcgc tttatgattt ccagtcaacc caacctgcca gaggatctcc
    19201 acccgcaaat aattgccaag cccagccaga aacgcctgat cgagcagtaa tcctgcaaac
    19261 tgacggttac gaaagcgcgg tgacaataac cgttccttta ccacctccgg cgtcagattc
    19321 ggatccagca catcgggacc aacgcgttgt aaaaacggat gcgtggtcag ttgttccggc
    19381 ctcaacatct caatatccga ggcgctataa agcagaatgg ttttgtcagc cgtttgcagt
    19441 tttacccgca atactcgcgt ggtctgcggc tcttcgccgg tatcaaccac gcgccagacg
    19501 ccgtaaagct gattatggct gtagagcgtt aagtcgttgg aaaaatgagt taacaacgcc
    19561 ttaccacgcg tttccacatg ggtaacgtgt tgaccgataa gttgtgattg ataagttttt
    19621 aactgcggga aggcaaacca gacatcagtt agtggtttgc ctttgatcgc cgcctccagg
    19681 ttatccgctg cacggcggat ctccgggcct tcaggcatgg tgatatcctt ttttcattct
    19741 ttattaatcg gggttatgct gcgacaacaa tctccttttc ggcaaatgta gagcgtagtc
    19801 ggcgggcgaa cgccagtgcg tgctcgccgt cgccatgcag gcagactgtt tgcgccgtga
    19861 ccgttgccca ttcgccggtg atacttttga ctctgccgtg ttgcaccatc tctagcgttt
    19921 gcgccagcgc ctgttcttcg ttttcaatca acgcgcctgg ctgacttcgc ggcaccagcg
    19981 agccgtcagc ctgataaccg cgatcggcaa acacttcctc gcgcgttgtc aggccatatt
    20041 gcttgcctgc acgaatcagc tcgcttcctg ccagcccaac gagaaccaat gctggatcgc
    20101 aagcgtatac cgctctggcg atggcgtctg ccagttgtgc ttctttcgcc gcctggttgt
    20161 acaacatgcc gtgcggtttg acatgacgca ttacgccgcc ttgcgcacgg gcaatcgttg
    20221 ccagcgcgcc aatttgatac agcgtctggg cgtaaacggt ttctggcggc agctgcatgg
    20281 cgctgcgacc aaaattttcc ctgtcgggaa aactcgggtg agcgccaatc gcgacaccat
    20341 tttttattgc ttcacgcacg caagcctgca tggtttgggc atcgcctgca tgaaatccac
    20401 aagcaatatt ggcagaggaa accagcgtta atagctctgc gtcgctggcg cagccttcgc
    20461 ccagatcggc gttcaggtca attttcattg tgtagccgcc acgctaattg ttcgaaataa
    20521 cgttgttgat cttgccgcgc tttcagtgcc tcttccagtg aacactggac aaaatgaatc
    20581 ggctgaccga gcggaatttg cgccagatgg tacatatcag cctcaatgat acaggcaata
    20641 cgcgggtaac caccggtggt ctgtgcgtcg ttcatcaaca caataggctg cccgttatgc
    20701 ggcacctgca ccacacccgg taacaaaccg tgagataaca gttcgcgatc ggtggtgcgt
    20761 tttaaaattt gcccctgtaa gcgatagccc atgcggttac tttgcgggct aagctgccag
    20821 ggcgaacgcc agaatgcatc ctgcgaggcg cgatcgaact catgatattc cggccccggc
    20881 aaggcgcgaa tgcggttgcc ccacagcagc tgtttaacgc cctgcgcttc cataaaatca
    20941 cgcttcgatt tgccaatcgg gagtcggtca ccatccttca gtaaacggcc ttccagcccg
    21001 ccaatcccca cgttgagatc ggtgctgcat gaccccatta ccggcggaac atcaatacca
    21061 cccgcgaccg ccagataact gcgcatcccg tgctgcgggc gtttaagcgt taaacgctgg
    21121 cctgctttca tcggcaatcg ccagccggtc cagacggcat tatcatccag ccgcgcttcg
    21181 caaccggcac ccgtcagagc aaaccaccca tcagtttcga actcaacagt taactgaccg
    21241 agcgtgatct ccagcgcggg ggcattggcg tcattaccca ccagtaggtt agcaatgcgt
    21301 aacgcgggca tatccagtgc gccgcagtgg ctgatacccg actggcgaaa accgtgacga
    21361 ccgccatcct gcacagtggt atacatgccc gcacgaataa tcttcagcat actccctcct
    21421 tctgcggtac aaagcgcacg ctgtctcccg gacgtaataa gatgggttcg tcacgcgccg
    21481 gatcaaacag gctgagtgag gtatgaccaa tcaactgcca gccacccggc gttgccagcg
    21541 gataaacacc agtctgcggc ccgccgatcc cgacagaacc tgccggaacg agtaagcgcg
    21601 gttcagcgcg ccgtggcgtg tgtagttgtt ccggcaaact cccgagatac gggaagcccg
    21661 gttgaaaacc taaaaaccag accacgtatt ccacggagga gtgcaattca acaacctgtt
    21721 tttcgctcaa cccgcaatgc gccgcgacca ccgccaaatc cggtccgcct gcaccaccgt
    21781 aaaccaccgg aatttcaata aagcgagact caggttccag ggcctcgctc tcctcccacc
    21841 agcgttgcaa acgctctatg gcatccagcg ccagcgactc aggattacgc aaaatcaccg
    21901 taatattgtt catgccggga atggcttcaa gcacattcgg catatccacc agacgctgcg
    21961 ccagtcgcca gatccgtttc tggctagcca gcgtcaccgg cggttccagt tccagcacta
    22021 ccgccgtttc acctatcaga taacaacgcg ctcgttgcac tttcgatccc tctgattatt
    22081 cgttatgcag gattaggaat atcaataaag gtcacatcaa gatcggtatt ttcattcagc
    22141 cactcgctca atgcgcgaat accaccacgt tcagtggcat ggtgacctgc agcataaaaa
    22201 tgcaatccct gctcgcgggc tgaatgaatg gtctgttcag aaacttcgcc agtaataaaa
    22261 gcatccacgc caaaacgcgc ggcgctatcg ataaaacttt gcccgccgcc cgtgcaccag
    22321 gcgacgcgct ggaccacctc aggtccggta tcgccacacc ataacggctt acgtcccaga
    22381 cgcgcttcaa tccaggaggc tagttccagt cccggcacag gcatggtcag ctctccccac
    22441 ggcaccaacg gctcaatttc gcccatgacc gtgatcccca gtaacgccgc cagctgtgcg
    22501 ttattgccca gctcaggatg tgcgtcaagc ggcaaatgcc agccatacag gttgatatca
    22561 ttcgccagca acgtttttaa acggttacgc ttcattccgc gaatgaccgg agactctcct
    22621 ttccagaagt agccatgatg cacaatgacc gcatccgcgc ccagacgcac tgcctcatcg
    22681 agcaaagcct ggctggcggt gacaccggta acaatttttt gcaccgtctc tttgccttcc
    22741 acctgcaaac cattcggcgc gtaatcacta atcgccgcgc tgttcagttt ttcgttaatc
    22801 agttgttcca gttcggtgtt tttcatcttc gcgccttccg tattctgata gctacaccgt
    22861 taacatagcg ttacctcgcc agttcgtcga tagccaatct tttgacatat taagaaacaa
    22921 acccgacgcc aaaagacgca aattgtttat atcatcacgc ttcagctaat gtatatttat
    22981 gcaatcagac agcatattgc ataaataatc agatctgtat caaagtttta actttaagga
    23041 aacacaagca cgccgcgcaa ctattacgtg acgttgccgt caattgtgga attattatga
    23101 ataaacacgc atcacagccg cgcgctattt actatgtcgt tgcgctgcaa atctgggaat
    23161 attttagctt ttacggcatg cgtgccctgc tgattctcta tctcaccaat caactaaaat
    23221 acaacgatac tcacgcctac gagttattta gcgcctactg ttcgctggtg tatgtcacgc
    23281 caatcctcgg tggctttttg gcggataaag ttctcggcaa tcgcatggcg gtgatgctgg
    23341 gggcgttgtt gatggcgatc ggtcatgtgg tgctgggtgc cagtgagatc catccgtcat
    23401 tcctctatct gtccctggcg attatcgtct gcggctatgg cctgtttaaa tctaacgtaa
    23461 gctgtctgct cggcgagctg tatgagccaa ccgatccgcg tcgtgatggc ggtttctcgc
    23521 tgatgtatgc ggcgggtaac gtggggtcta ttatcgcacc tatcgcctgt ggttacgccc
    23581 aggaagagta cagctgggcg atgggctttg gcctggcggc ggttggcatg atcgcgggtc
    23641 tggtcatttt cttatgtggc aatcgtcatt tcactcatac ccgcggcgtt aacaaaaaag
    23701 tactgcgtgc gacaaacttt ctcctgccga actggggatg gctgctggtt ctgctggtgg
    23761 caacgcctgc actgattacc gtactgttct ggaaagagtg gtcggtatac gccttaattg
    23821 tcgcgactat tattggcctg ggtgtactgg caaaaattta tcgcaaagca gaaaaccaga
    23881 aacagcggaa ggagctgggg ctgattgtga cgctcacctt cttcagtatg ttgttctggg
    23941 ccttcgcaca acagggcggt agctcgatta gcctttatat cgaccgcttc gttaaccgcg
    24001 atatgtttgg ttataccgtt ccgaccgcga tgttccagtc gattaatgcc ttcgcagtta
    24061 tgctgtgcgg tgtgttcctg gcgtgggtgg taaaagagag tgtcgcgggt aatcgtaccg
    24121 tgcgcatctg ggggaaattt gctcttggcc ttggcctgat gagcgccgga ttctgcattc
    24181 tgaccttaag cgcccgctgg tccgcaatgt atggtcactc ttctctgcca ctgatggtat
    24241 taggcctggc ggtgatgggc tttgcggaac tgtttatcga cccggttgcc atgtcgcaaa
    24301 ttacgcgcat tgaaatcccc ggtgtgaccg gcgtattaac cggcatctac atgctgcttt
    24361 ctggcgcgat cgcgaactat ctggcgggcg tgattgccga tcagacatcg caggcttcgt
    24421 ttgatgcttc cggggcgatc aactactcca tcaatgcata tattgaagta tttgatcaaa
    24481 ttacctgggg cgcactggcg tgtgtaggac tggtactgat gatttggctg tatcaggcgc
    24541 tgaaattcag aaaccgcgcg ctggcgctgg agtcataata ggccagcacc gatgcctgat
    24601 gcgacgcttg acgcgtctta tcaggcctac gtatttcctg caatttattg aatttgcaca
    24661 aatttgtagg ctggataagg cgttcacgcc gcatccggca tctgggctcg attgcctgac
    24721 gcgtctgtta tttccccttc cgcgccgcct catacgctgc caacgtttgt actctcgctt
    24781 ctttgtgctc gactatcggt tgcggataat ccagcgtcac acctgctttc tgcgcccact
    24841 tccacggctc atgcaccact ttccctggca catcgcgcag ttccggtagc cactggcgga
    24901 taaactcgcc ctcatggtca aatttctcgc cctgagttat cggattgaaa atacgaaaat
    24961 acggcgcagc atcagtaccc gtagaagccg cccactgcca gccaccgtta ttagctgcca
    25021 aatcaccatc aatcagctgc gacatgaaat atcgctcgcc ttcgcgccag tcgatcaata
    25081 aatctttcac cagaaaactg gctgtaatca tccgtagccg gttatgcatc cagccagtgc
    25141 tgttaagctg acgcatggca gcatcgacaa tcgggtatcc cgttttgcct tcctgccagg
    25201 cctgtaaatg tgcgggattg ctctgccact gcacacgatc cgtccaggca ataaacggac
    25261 aatgtttaca caacgagggg taatacgtca tcagatgacg gtagaactcg cgccagatca
    25321 gctcactaag ccagacacta ccggccccac cgtccagcgc ctgcggctgt tccgccaaca
    25381 agcgatgcaa acactggcga ggcgataacc cgcccgttgc caggctggcg gacaaacgac
    25441 tggtgccttc cactgccgga aaatctcgtt gttgctcata ttctccggca ccgttctggc
    25501 aaaactggcg taattgcgca atcgccgctt tttcttccac cgggaaatgc gcagtatcga
    25561 aagactgacg aggataattc agcgtaatgg atggcgcggg ctttatcgat ccgctactac
    25621 gaacttttgg tgcagcgacg cactccggca tcccttcccg cagccgtttc agccaggcat
    25681 tcttaaaagg cgtaaagact ttgtacatct cgtgattacc ggtcatcacc gcgccaggcg
    25741 gcaggatcac gctgtcatca aatccttcac acaccacgtt acgcagcgct ctttcaactt
    25801 ccacatcccg cgcccgctca ttcacttcat actgatagtt ataaaacaag tgggtaacgc
    25861 tgttttccgc gcacacctgt ttaactattt cgacactggc gacaaagtca tccacttcac
    25921 ggaacaataa aggaatacct ttttccgcaa gcgctatttg tagcccattc agttgagcat
    25981 tgataagttc agcctgacgc ggcgacatgt tatgcgtcgc ccactggcgt ggtgtagcga
    26041 tatacaacgc cagcacgcgt gcagacgaat tgcggcaggc ggcagccagt gcgagattat
    26101 cgtgcagacg taaatcctgg cgaaaccaga ccagatgggt agtcataaaa ctcctgaatg
    26161 gcgcaagtta atcacacgcg gccaaagttc gaaataacgc tgccctgaaa gccaggcgtc
    26221 aggataaagc gccatatagt gtttgatacg catcagcggc gcaagaagtg gttgctcgcc
    26281 acggcgataa ctgtcgataa gcgcagccag ctgctggcgt tctgtggaat caatatgcgg
    26341 gcgaaaataa ccctgaacgt gcatcaaaac attggtgtga tcgcgcgggt tggcggggtg
    26401 agaaagcaaa acaactacgc gttggcggta ctggttgtaa tagtcatcca gattttgcca
    26461 ctggtgaata tcagccacga acgggccaag tttgcggtac tccggctgag aatgcgccag
    26521 aaagacgagt ttatagcggc tgtgataatc gagaagcgcc ccgcgcgtta agccatgttc
    26581 tttcagtcga tgtaactcat gaagggcaaa gatacggatt agggtgctgt catcaaatcg
    26641 ttgta
//
LOCUS       DAOFQD010000065        26000 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.065, whole genome shotgun sequence.
ACCESSION   DAOFQD010000065 DAOFQD010000000
VERSION     DAOFQD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.065"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(172..1023)
                     /gene="hchA"
                     /locus_tag="QBF46_002728"
     CDS             complement(172..1023)
                     /gene="hchA"
                     /locus_tag="QBF46_002728"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDQ6674890.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDDGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            1278..1473
                     /locus_tag="QBF46_002729"
                     /pseudo
     CDS             1278..1473
                     /locus_tag="QBF46_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1613..2827)
                     /gene="ompC"
                     /locus_tag="QBF46_002730"
     CDS             complement(1613..2827)
                     /gene="ompC"
                     /locus_tag="QBF46_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDQ6674891.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDAGSDGDKSYARIGFKGETQIADQFTGYGQWEYNLQANGTEGDGDNSATRLAFAGL
                     GFGQNGTFDYGRNYGVVYDIEAWTDMLPEFGGDTYAGADNFMNGRTNGVATYRNNGFF
                     GQVDGLNFALQYQSNNESGSDGLFGQEGSGSGDGRSLAKENGDGFGMSTSYDFDFGLS
                     LGAAYSNSDRSNNQAARGYGDGNHFYGNSYAGGGTAEAWTVGAKYDANNVYLAAMYAE
                     TRNMTAYGSSDSHSADGKLGGGGIANKTQNFEVVAQYQFDFGLRPSIGYLQSKGKDLG
                     GQEMYNNGNYHYTNKDLVKYVEVGATYYFNKNMSTYVDYKINLLDNDDDFYANNGIAT
                     DDIVAVGLVYQF"
     gene            3394..3759
                     /locus_tag="QBF46_002731"
     CDS             3394..3759
                     /locus_tag="QBF46_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDQ6674892.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVISRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            3799..4494
                     /locus_tag="QBF46_002732"
     CDS             3799..4494
                     /locus_tag="QBF46_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDQ6674893.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDYKVIDAFSSAG"
     gene            4561..5979
                     /gene="dcm"
                     /locus_tag="QBF46_002733"
     CDS             4561..5979
                     /gene="dcm"
                     /locus_tag="QBF46_002733"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDQ6674894.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDSRRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSITRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            5960..6430
                     /gene="vsr"
                     /locus_tag="QBF46_002734"
     CDS             5960..6430
                     /gene="vsr"
                     /locus_tag="QBF46_002734"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDQ6674895.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYCCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(6419..7339)
                     /gene="yedA"
                     /locus_tag="QBF46_002735"
     CDS             complement(6419..7339)
                     /gene="yedA"
                     /locus_tag="QBF46_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDQ6674896.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVTPVIQDASSE"
     gene            7512..8429
                     /locus_tag="QBF46_002736"
     CDS             7512..8429
                     /locus_tag="QBF46_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDQ6674897.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            8508..8690
                     /locus_tag="QBF46_002737"
     CDS             8508..8690
                     /locus_tag="QBF46_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDQ6674898.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            8928..10622
                     /gene="yedQ"
                     /locus_tag="QBF46_002738"
     CDS             8928..10622
                     /gene="yedQ"
                     /locus_tag="QBF46_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDQ6674899.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLPHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLYQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISVSLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(10619..11434)
                     /locus_tag="QBF46_002739"
     CDS             complement(10619..11434)
                     /locus_tag="QBF46_002739"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDQ6674900.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWGEGLDHFFSAR"
     gene            complement(11732..11959)
                     /gene="yodD"
                     /locus_tag="QBF46_002740"
     CDS             complement(11732..11959)
                     /gene="yodD"
                     /locus_tag="QBF46_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDQ6674901.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            12122..12310
                     /gene="dsrB"
                     /locus_tag="QBF46_002741"
     CDS             12122..12310
                     /gene="dsrB"
                     /locus_tag="QBF46_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDQ6674902.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(12354..12977)
                     /gene="rcsA"
                     /locus_tag="QBF46_002742"
     CDS             complement(12354..12977)
                     /gene="rcsA"
                     /locus_tag="QBF46_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDQ6674903.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13267..14052)
                     /gene="fliR"
                     /locus_tag="QBF46_002743"
     CDS             complement(13267..14052)
                     /gene="fliR"
                     /locus_tag="QBF46_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDQ6674904.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGVALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            complement(14061..14330)
                     /gene="fliQ"
                     /locus_tag="QBF46_002744"
     CDS             complement(14061..14330)
                     /gene="fliQ"
                     /locus_tag="QBF46_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDQ6674905.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(14340..15077)
                     /gene="fliP"
                     /locus_tag="QBF46_002745"
     CDS             complement(14340..15077)
                     /gene="fliP"
                     /locus_tag="QBF46_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDQ6674906.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(15077..15442)
                     /gene="fliO"
                     /locus_tag="QBF46_002746"
     CDS             complement(15077..15442)
                     /gene="fliO"
                     /locus_tag="QBF46_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDQ6674907.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(15445..15858)
                     /gene="fliN"
                     /locus_tag="QBF46_002747"
     CDS             complement(15445..15858)
                     /gene="fliN"
                     /locus_tag="QBF46_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDQ6674908.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(15855..16859)
                     /gene="fliM"
                     /locus_tag="QBF46_002748"
     CDS             complement(15855..16859)
                     /gene="fliM"
                     /locus_tag="QBF46_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDQ6674909.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(16864..17328)
                     /gene="fliL"
                     /locus_tag="QBF46_002749"
     CDS             complement(16864..17328)
                     /gene="fliL"
                     /locus_tag="QBF46_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDQ6674910.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(17433..18560)
                     /gene="fliK"
                     /locus_tag="QBF46_002750"
     CDS             complement(17433..18560)
                     /gene="fliK"
                     /locus_tag="QBF46_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDQ6674911.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLQVPVSLQVRVTGNSGVDIFA"
     gene            complement(18557..19000)
                     /gene="fliJ"
                     /locus_tag="QBF46_002751"
     CDS             complement(18557..19000)
                     /gene="fliJ"
                     /locus_tag="QBF46_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDQ6674912.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(19019..20392)
                     /gene="fliI"
                     /locus_tag="QBF46_002752"
     CDS             complement(19019..20392)
                     /gene="fliI"
                     /locus_tag="QBF46_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDQ6674913.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(20392..21078)
                     /gene="fliH"
                     /locus_tag="QBF46_002753"
     CDS             complement(20392..21078)
                     /gene="fliH"
                     /locus_tag="QBF46_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDQ6674914.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(21071..22066)
                     /gene="fliG"
                     /locus_tag="QBF46_002754"
     CDS             complement(21071..22066)
                     /gene="fliG"
                     /locus_tag="QBF46_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDQ6674915.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(22059..23717)
                     /gene="fliF"
                     /locus_tag="QBF46_002755"
     CDS             complement(22059..23717)
                     /gene="fliF"
                     /locus_tag="QBF46_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDQ6674916.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            23932..24246
                     /gene="fliE"
                     /locus_tag="QBF46_002756"
     CDS             23932..24246
                     /gene="fliE"
                     /locus_tag="QBF46_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDQ6674917.1"
                     /translation="MSAIQGIEGVISLLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            24580..24912
                     /gene="emrE"
                     /locus_tag="QBF46_002757"
     CDS             24580..24912
                     /gene="emrE"
                     /locus_tag="QBF46_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDQ6674918.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            25081..25632
                     /gene="ybcL"
                     /locus_tag="QBF46_002758"
     CDS             25081..25632
                     /gene="ybcL"
                     /locus_tag="QBF46_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDQ6674919.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            25642..>26000
                     /locus_tag="QBF46_002759"
     CDS             25642..>26000
                     /locus_tag="QBF46_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDQ6674920.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCW"
BASE COUNT         6354 a         6443 c         6690 g         6513 t
ORIGIN      
        1 tgcttcatat cactatctca ataagcataa tgtgttccga attacgttac cgcaaagaaa
       61 ttaacatagg gcttcagtac gccataaatt aaccgtgaaa aaacgcagcc acgtcgtgag
      121 tactaacgcg gctgcgatta attatgcgct tacattcaaa cgtaacaggg attaacccgc
      181 gtaagctgcc agcatttcct gcgccgccag tttacccaac gcattcgctg cgaaaggact
      241 gtcgccggtg agaagtttac ggtccttatg tactcgcccg gtgatgtcgt cattaatgat
      301 gttcatgccc attttcttca gctcttcgcc gaagtaccag gtgagatgac ctggcatata
      361 gccaatatct ggcgtttgtt tgtctgcggc gtctgggaat gcgcaaatgg aataaccatt
      421 cagtgggtta tcgccgtggc gaagcgccag aaaagccgcc gggccgtggc aaagggagat
      481 aacaaaacgg tcatttttga ttgcccactg taaagcggca gctacgtcct ggctttcagg
      541 taaaccaata agtgcgccat gaccaccagg aacaaagatt gctgcatatt cgctatcagc
      601 gttgaggctg gcgacaacat ccgcgagttt cttcggattg cggaacaacg atttatgctg
      661 ctcaaagaat ggcattactt tttcatcttt gtgcggcata gcccagtatt caaacttggt
      721 catcagaccg gaaatggtcg ccacttcgaa ttcgaaacct gcagcatgga gatgatacaa
      781 cggcagcaac gtttcaatcg gatggttacc ggtcgagaac agttttccgt catcggttgg
      841 caaataacgt tcgtccgcgg cgatcaccag aattttatgc ttaccgcgat acggttttgg
      901 atagtccacg ccatcaagat cagagacggg actggtatat tggctaagcg aatattctga
      961 agggaagaat gcattatctt cagcaatatc gacctgcgga tttttacttg tttgaacagt
     1021 catagtgtat tccttattgt tgcttagtag ggtagtcact atattttcga ctgagctggt
     1081 cgctattttt aaccagtctg gaaaattgag tcaatatatc gaaagcggta gtcggcgggt
     1141 gtaagtatgt aatgatgatg ctgcactggt gttgaatatt attcacgcca aattttcctt
     1201 attgagcaac gtcgtcgcaa gtggcctcaa tgattttaga ccattatcaa aggtacgggg
     1261 cggcggggag agatttagtg ctgtgaaata ttgtggtact gatgaatatt tatatcgcgg
     1321 attggtagag aaaagattat ttcatgcact atttttgcaa atatttctct atgggaagcg
     1381 tgtttgttac ggattaattc ataaagaaat gcatttgggt gttggtttga agagtcgctg
     1441 gttgatgtta ttgtgccagg aggggcaggg taaggtgctg tatcgatatg cattggcaat
     1501 tgcgtgtgcc gcagatggag atgaaatgcc aataaaaaag cctgaattcg atgaggaacg
     1561 aactcaggct agtgtttagt tttacagtaa cacgccgcag cacgttggct gattagaact
     1621 ggtagactaa gcccacagca acgatatcat cggtagcgat gccgttgttt gcgtagaagt
     1681 catcgtcgtt gtccagcagg ttgattttgt aatcaacata ggtggacatg tttttgttaa
     1741 agtagtaggt tgcaccaacc tcaacgtatt taaccagatc tttattggta taatgataat
     1801 taccattgtt gtacatttct tgaccaccta aatccttacc tttagattgg aggtaaccaa
     1861 ttgaaggacg caggccgaaa tcaaattggt actgagcaac aacttcaaag ttctgagttt
     1921 tgttagcgat accaccgccg ccaagtttcc catcggctga atgagagtcg ctgctaccat
     1981 aagcggtcat attgcgagtc tctgcataca ttgctgctaa atatacattg ttggcatcgt
     2041 atttagcacc aacagtccac gcttctgcag taccaccacc tgcatagcta ttaccataga
     2101 aatgattgcc atctccatac ccacgagcag cttggttatt ggaacggtca gaattagagt
     2161 aagcagcacc cagacttaaa ccgaagtcga agtcgtagga agtagacata ccgaaaccat
     2221 cgccattttc tttagcaaga ctacggccat ctccgctgcc agatccttct tggccaaaga
     2281 gaccgtcact gccactttca ttattactct gatattgcag cgcaaagttc aagccatcaa
     2341 cctgaccaaa gaagccattg ttacggtagg ttgcaacacc gttggtacga ccgttcatga
     2401 aattgtcggc accagcatag gtatcgccac caaattctgg cagcatatcg gtccatgctt
     2461 cgatgtcata tacgacaccg tagttacgac cataatcgaa agtaccattc tgaccaaaac
     2521 ctaaacctgc gaaagccaga cgggtagcag agttatctcc atcgccttct gtgccatttg
     2581 cttgcaggtt gtattcccac tgaccataac cagtgaattg gtcagcgatc tgagtttcac
     2641 ctttgaaacc gatacgggca tatgacttgt cgccatcgct gccagcatca tcagagaagt
     2701 agtgcaggcc cgctactttt ccgtacaaat ccagtttatt accatcttta ttatagactt
     2761 cagccgcatt tgctgcgcca gcaactaata acgccgggac cagcattgcc agaacttttc
     2821 ttttcattat gtattccctt gtgattataa tcttcatgaa tatatcaata agtgccgtta
     2881 tccaaaaaaa agcacatttg gatactattc tatgaagttc attttatttt aaggattaca
     2941 tgtaataaat gtatttaaaa tatttcaaaa tgtttctaat ctatttaata taattcagaa
     3001 ttatgaattc taaatataaa tattcatgaa aattataaat gtatatcaat atgttatgag
     3061 ttgagcggaa acataggggg tatcaatgat tgttgcgcca aacggctggt gtgatcaggc
     3121 gcacattaat gaaggcatat aaaaaagccg gcatcatgcc ggcccaaaaa atataaacaa
     3181 gacgataaaa ggcgaataac ggaagcatca tgacacagaa taataaaaag gtataattgt
     3241 aatcagtgct aatatattga ttaaatgaat taataaagcc gatatcaaag ataaataaaa
     3301 tcgctcaaaa cgatttttcc tgtattcact gccgttgcgc aaaatttatc tatttgttca
     3361 aaaaatgatt gagtcttgac tggctcatcc aatgtggaaa aatgtgactt ttatcacata
     3421 atagtactaa gtctgaattt tccgggttat ctcaaaatgg aatacggttc gacaaagatg
     3481 gaagagagac tctctcgcag ccctggcggc aaactggctt tgtgggcttt ctatacatgg
     3541 tgtggctatt tcgtctgggc aatggcgcgt tacatctggg tgataagccg gatccccgat
     3601 gcccccgtgt cgggatttga aagcgattta ggctctaccg cgggaaagtg gttaggggca
     3661 ctggtcggat ttttatttat ggctctggtc ggggcattgt taggcagcat tgcctggtac
     3721 acccgacctc gtcccgcacg tagcagacgc tatgaataaa aactgctaag gtggcgactc
     3781 tttggcaacg gagcgcgtat ggacttacaa cactggcagg cacagttcga aaactggttg
     3841 aaaaaccatc atcagcacca ggacgcggcc catgacgtgt gtcattttcg ccgcgtctgg
     3901 gctacggcac agaagctggc ggcagatgac gatgtcgata tgctggtgat tttaaccgcc
     3961 tgttattttc acgatattgt gagcctggcg aaaaatcatc cgcaaaggca gcgttcttca
     4021 atcctggcgg cagaagagac gcgtcgcctg ctccgtgaag agttcgagca atttccggcg
     4081 gagaaaatcg aggccgtttg tcatgccatt gccgctcaca gtttcagcgc gcaaatcgcc
     4141 cccttaacaa cggaagctaa aattgtccag gatgcggatc ggctggaagc cctgggggcg
     4201 attggcctgg cgcgcgtgtt tgccgtttca ggggcattgg gcgtggcgct gtttgatggt
     4261 gaagatccgt tcgcacagca tcgcccgctt gatgataaac gctacgcgct ggatcatttc
     4321 cagactaagt tgctgaagct accgcaaacc atgcaaaccg caagaggcaa gcagctggcg
     4381 cagcacaacg cgcatttttt agtcgagttt atggcgaagc tcagtgccga actggcgggg
     4441 gagaatgaag gtgtcgatta caaggtgata gatgcgtttt catccgctgg ctgagcgcgt
     4501 ggccctaaat ggctgtaatt atgttaacct gtcggccatc tcagatggcc ggtgaaatct
     4561 atgcaggaaa atatatcagt aaccgattca tacagcaccg ggaatgccgc acaggcaatg
     4621 ctggagaaac tgctgcaaat ttatgatgtt aaaacgttgg tggcgcagct taatggtgta
     4681 ggtgagaatc actggagcgc ggcaatttta aaacgtgcgc tggcgaatga ctcggcatgg
     4741 caccgtttaa gtgagaaaga gttcgcccat ctgcaaacgt tattacccaa accaccggca
     4801 catcatccgc attatgcgtt tcgctttatc gatctattcg ccggaattgg cggcatccgt
     4861 cgcggttttg aatcgattgg cggacagtgc gtgtttacca gcgaatggaa caaacatgcg
     4921 gtacgcactt ataaagccaa ccattattgc gatccggcga cgcatcattt taatgaagat
     4981 atccgcgaca tcaccctcag ccataaagaa ggcgtgagtg atgaggcggc ggcggaacat
     5041 attcgtcaac acattcctga acacgatgtt ttactggccg gtttcccttg tcagccattt
     5101 tcgctggctg gcgtatcgaa aaagaactcg ctcgggcggg cgcacggttt tgcctgcgat
     5161 acccagggca cgctgttttt tgatgtggtg cgcattatcg actcgcgtcg tccggcgatg
     5221 tttgtgctcg aaaacgtcaa aaacctgaaa agccatgacc agggtaaaac gttccgcatc
     5281 atcatgcaga cgctggacga actgggctat gacgtggctg atgcagaaga taacgggcca
     5341 gacgatccga aaatcatcga tggcaaacat tttctgccgc agcaccgtga acgcatcgtg
     5401 ctggtgggtt ttcgtcgtga tctgaatctt aaagccgatt ttactctgcg tgatatcagc
     5461 gaatgtttcc ctgcacagcg agtgacgctg gcgcagctgc tggacccgat ggtcgaggcg
     5521 aaatatatcc tgacgccggt gctgtggaag tacctctatc gttatgcgaa aaaacatcag
     5581 gcgcgcggta acggcttcgg ttatggaatg gtttatccga acaatccgca aagcatcacg
     5641 cgtacgctgt ctgcgcgtta ttacaaagat ggcgcggaaa ttttaatcga tcgcggctgg
     5701 gatatggcca cgggtgagaa agactttgac gatccgctga atcagcaaca tcgtccacgt
     5761 cggttaacgc ctcgtgaatg cgcgcgctta atgggttttg aagcgccggg agaagcgaaa
     5821 ttccgcattc cggtttcgga cactcaggcc tatcgccagt tcggtaactc ggtggtcgtg
     5881 ccggtctttg ccgcggtggc aaaactgctt gagccaaaaa tcaaacaggc ggtggcgttg
     5941 cgtcagcaag aggcacaaca tggccgacgt tcacgataag gccactcgca gcaaaaatat
     6001 gcgcgcgatt gccacgcgtg atacggcgat tgagaagcgc ctcgccagtc tgttaaccgg
     6061 gcagggcctg gcgtttcgcg ttcaggacgc cagtctgccc ggacgtccgg attttgtcgt
     6121 tgatgaatat tgttgcgtga tatttaccca cggctgcttc tggcaccatc atcactgcta
     6181 tctgtttaaa gtgcctgcga ctcgaaccga gttctggctg gagaagatag gtaaaaatgt
     6241 tgagcgcgat cgccgcgata tcagtcgctt gcaggaactc ggctggcgcg tattgattgt
     6301 ctgggagtgc gcgttacgtg ggcgcgagaa gctgacggat gaagcgctta gcgagcgtct
     6361 ggaagagtgg atctgcggcg aaggtgccag cgcgcagatc gacacgcagg ggattcattt
     6421 actcgcttga tgcgtcctga ataactggcg taactacggg ttttgccggg aagagatatt
     6481 ttcccaacgt gaccagtacc accgcgaaga caattacgcc gagcgccagc cattcaatct
     6541 tcgacagcgt ttctccgccc agtcccgtac ccagcaagac cgcgaccacc gggttaacgt
     6601 aggcgtaact ggtggcgaga gccggactga cgttgcggat taaatacata taagcgttaa
     6661 tggcgataat cgaaccaaac agcgccagat agccgaccgc aaggaagcct gaaagggaag
     6721 ggagcgccgt cagtttttca cctgcaatca tcgacgcgat cattaacacc acgcctgccg
     6781 ccagcatctc aatcgcaccc gccatcatcc ctacaggtaa ggtaatgcgc gagccataaa
     6841 ccgagccaaa cgcccagcta atcgagccga ttaaaatcag aatcgcgccc cacggattgc
     6901 cgcttaaatt tccaccgcta ttgagcatga tgattccggc aagcccaatg gcaataccca
     6961 cccattccag tttgcgcgtt ttaatgccaa acaggcggct gaagcacagg gtaaagaggg
     7021 gcacggttgc aaccactacg gcggcgatgc cggaaggaac attttgatgt tcggcaaccg
     7081 tcaccatgcc attaccgaca gccagcaata acaggccaat cagcgcggca ttgagcagcg
     7141 gacgtagcgg ggggagtttg tgtccgcgca gtagtaaaaa tgccagcaat aaaataccgg
     7201 ctgccaggaa tcgaacgccc gccatcatta acggaggcca gctttccacg ccaatccgaa
     7261 tgacaaaata ggttgagccc caaatgatat acaacgcaaa cagcgcgcca aaaagcggta
     7321 ataactggcg gaaacgcata atccctcacg gtggaaataa aaaggtggtt catagtaaac
     7381 gtgaaaatca ttctgctggc gagagatata attgcacttg attgttaaaa aaatgttgac
     7441 ctgtgaagca agtatcagag ctgccgtttt tgcttcatac ttacaccctt caacaataaa
     7501 aatgagaggg aatgcttttg gccgggagta gtttactgac gttgctcgat gatatcgcca
     7561 cactgctgga cgatatctcc gtgatgggca aactggcggc gaagaaaacc gccggtgtat
     7621 taggggatga cttatcgctc aatgcgcaac aagtttcagg cgtgcgggcc aaccgggaac
     7681 ttcccgtggt ctggggcgtg gcgaaaggat cgctgattaa taaagtgatt ctggtgccgc
     7741 tggcgctgat catcagtgcg tttatcccgt gggcgattac gcctctgttg atgattggtg
     7801 gcgcgtttct ctgctttgaa ggggtagaga aagtgctgca tatgctggag gcgcgtaaac
     7861 ataaagaaga tccggcgcag agccagcagc gtctggagaa gctggcggcg caggatccgc
     7921 tgaagtttga aaaggacaaa ataaaagggg cgattcgtac cgattttata ttgtctgcgg
     7981 aaatcgtcgc catcacgctg gggattgtgg ccgaagcgcc gttgcttaat caagtgctgg
     8041 tgctttcagg catcgcgctg gtagtgaccg tgggcgtcta cggtctggta ggggttatcg
     8101 ttaagattga tgacctgggg tattggctgg cggaaaaatc cagcgcgctg atgcaggcat
     8161 taggtaaagg gttattgatc attgcgccct ggctgatgaa agcgttatcg attgtcggca
     8221 cgctggcgat gttcctcgtc ggcggtggga ttgtggtaca tggaattgcg ccgctgcatc
     8281 acgccattga acatttcgcc gggcagcaaa gtgcagtggt ggcgatgata ttaccgactg
     8341 ttttaaatct gattcttgga tttatcatcg gcggcatcgt ggtgctggga gtgaaagccg
     8401 tagcgaaaat acgcggtcag gcacattaat actcgcacag gctattatgc aaaattcgcc
     8461 atcttcaact aaggtgaaca agtttcactc gaaaaaagga ggcgagtatg agctttatgg
     8521 ttagtgagga agttacggtt aaagagggcg gcccgcggat gattgtcacc ggatactcca
     8581 gcggtatggt tgagtgtcgt tggtatgacg gttacggggt caagcgggaa gcttttcatg
     8641 aaaccgagct tgttccgggg gagggaagtc gtcctgcgga agaagtttga aagggtatca
     8701 ttaacacccg accggatgtg gcgctcacgt cgcatccggt cgggtgtgcc agtgtctgca
     8761 ctgaattgcc tgatgcgacg ctatcgcgtc ttatcatgcc tacgttataa aactatcgat
     8821 gtctgtcgcg tcttatcatg ccaccattat aaaattatcg ctgttttgcg gtacgctatc
     8881 gccaggttat gccagaatca taaaaaagca ggttgggagt cgtcagggtg cagcacgaga
     8941 caaaaatgga aaaccagagc tggttgaaaa aactcgcacg ccgcctgggg cctggtcatg
     9001 tcgttaatct ctgctttatc gtggtattgc ttttttccac cttgctcacc tggcgtgaag
     9061 tagtggtgct ggaagatgcc tatatctcca gccagcgtaa tcatctggaa aacgtagcca
     9121 acgcgctcga taagcattta cagtataacg tcgacaaact gatctttttg cgtaatggca
     9181 tgcgcgaagc tctcgtagcg ccactggatt tcacttcact gcgtaatgct gtaaccgagt
     9241 tcgaacagca tcgcgacgag cacgcctggc aaattgaact caaccgacga cgcaccctgt
     9301 cagtcaatgg cgtatcggat gcattagtca gcgaggggaa tctcctgtct cgcgaaaatg
     9361 aaagcctcga caatgaaatt accgctgcac tggaagttgg ttacttgctg cgactgccgc
     9421 acaacacctc gtcgatggtt gaacaggcga tgtatgtctc gcgtgccgga ttttacgttt
     9481 cgacgcagcc gaccttgttt acgcgcaatg taccaacgcg ttattacggc tatgtcaccc
     9541 aaccctggtt tatcggccat tcgcaacgag aaaatcgtca ccgcgcggta cgctggttta
     9601 cttcgcaacc ggaacacgcc agcaatactg aaccgcaggt taccgtcagt gttccagtag
     9661 acagtaataa ctactggtat ggcgtgctgg ggatgagtat tcccgtgcgt accatgcagc
     9721 aatttttaag aaacgccatc gataaaaacc tagatggtga gtatcagctc tatgacagta
     9781 agctgagatt tttgacctct tccaatcctg accatccaac agggaatatt tttgatcctc
     9841 gtgaactggc cttgctggcg caggcgatgg aacatgacac gcggggcggc attcgtatgg
     9901 acagtcgcta tgttagctgg gaacgtctgg accatttcga cggtgtgctg gtgcgcgtcc
     9961 atacgttaag cgaaggcgtg cgcggcgatt tcggcagtat cagcattgca ttaaccctgc
    10021 tgtgggcgct ctttaccacc atgttactca tctcctggta tgtgattcgc cggatggtta
    10081 gcaacatgta tgttctgcaa agctcgttgc agtggcaggc gtggcacgac accttaacgc
    10141 gtttatataa ccgtggcgca ctgttcgaaa aagcccgtcc gctcgctaaa ttgtatcaga
    10201 cgcaccaaca tcctttttct gtcattcagg tcgatcttga ccattttaaa gcgattaatg
    10261 accgctttgg tcatcaggcg ggcgaccgtg ttctttctca tgctgccgga ttaattagca
    10321 gttccttgcg tgcgcaggac gttgccgggc gggtcggtgg tgaggagttt tgtgtgattc
    10381 tgccaggcgc gagtctgacg gaggctgcgg aagtcgcaga acgtattcgc ctgaagttaa
    10441 atgaaaaaga gatgttgatt gctaagagta cgacgatacg catcagtgtc tcgctggggg
    10501 taagtagcag cgaggaaacc ggtgattatg attttgaaca actccagtca ctggctgacc
    10561 gtcgacttta tctcgctaaa caggccgggc gtaatcgggt atgcgcgagc gataacgctt
    10621 aacgggcgga gaaaaaatgg tccagccctt caccccagcc ttccggtcct tcacgctgcg
    10681 ttcgccagac gcgggccgga tcctcatcat gcagatgcac cccttcacgg tttagccctt
    10741 tcacaatcac cgcgtaatcc attacctcca gtaagggcgc atcgtttggc ccatcgccca
    10801 ggccaagtgt ggttgggcgt ttgcctgaca attgttgata ggtcgcgata atccagttgg
    10861 cagcctgatc ttttccggca gaggcatcca gtacgtgcca gaagcgcgca ccctgcataa
    10921 actgtaagcc aagttcgttc agacgagcgg taaattgtgc catacgctcg tcactgtcgc
    10981 gccagattag cgttaccgac gcctcatgca gctgcgtcag cgccgcctgg ctacggctta
    11041 atcccgtcca ttcggcgatg gttgcatcgt cgacatcatc gaaagtcgtg aatttaaaat
    11101 gttctttctc gcgtagcgta tttaaaacct ggctgatttc gccatggcta atacctgaga
    11161 tgatgcgtgg aaaaccgtct atatcctgcc attgttcagc aagctggatc actgcgccat
    11221 tttcggcaat cagcggtaac ccttgtagcc ccaacgtttt ttgcaagtac agcatttccg
    11281 ctgaagtttt actgctacag agaatgacgg gaacattcgc ttcgcgtaaa cggctgagcc
    11341 agggggcagc cggttgccag tcataactat gactgtccag cagggtgcca tcaagatcgc
    11401 taaaaaccag tagtggttgt tgaattgaaa acatggtcgc gcagttctcc ccttaccagg
    11461 atgattctca aaacagcaca aataatcaaa gatgattttt tcgggagcga tcccgttgac
    11521 gccagagcca ccaaggggtg acgcacgtca catttctatt cataagtagc gttaatcatt
    11581 catatcgcga atattttctt gtcagcgaaa aaaattgcgg ataaggtgat gaacacatca
    11641 gatttcctgg tgtaacgaat tttttaagtg cttcttgctt aagcaagttt catcccgacc
    11701 ccctcagggt cgggattttt ttattgtgca ttcaacgatt cacttcagag acgctgaagt
    11761 catgaatatc cagttcgaag gcatccgcca attcacgcca tgtatgatat tcacgtccat
    11821 cgacgctaac gtgttcagaa tcgttctggc tgcgatgaac ttcgctttcg ctaatttcat
    11881 tgatcgccgc cagcagggca tcgacatcga cgctgacctc acgttttgcg gtatcgctgt
    11941 actcttttgc ggttttcatc atctttcctc gcaaccgtta ctctgttgtt gccgccagtc
    12001 gttaagtccg cctggcatct cttaagtata gaccgtcgag aaaatcagca ctcgcgcgcg
    12061 cctggcgctg ccagaaaaac gaaattgttc tacactggca caaagccaca ggaggaaaac
    12121 gatgaaggtg aatgatcggg taacagtcaa aacggatggc ggtccgcgtc gtcctggcgt
    12181 ggtactggca gttgaggagt ttagtgaagg cacaatgtac ctggtttcgc tggaagacta
    12241 cccgctcggc atctggttct ttaatgaagc agggcatcag gacggtatct ttgtggagaa
    12301 agcagagtaa tcttattcag cctgactggt gggaaaccac cagtcagaat gtgttagcgc
    12361 atgttgacaa aaataccatt agtcacatta tccgtcagtc ggacgacatg gtagataacc
    12421 tgtttattat gcgttttgat cttacgttta atattacctt tatgcgatga aacggtcttg
    12481 gctttgatat tcatttggtc agagatttga atggttccct gacctgccat ccacattcgc
    12541 aacatactcg attcggttcg gctcaatgat aacgtcggca tatttaaaaa agaggttatc
    12601 gttgtctctt ttttcagaat atcgccaagg atatcgtcga gagattccgg tttaatcgat
    12661 ttagaactga tcaataaatt ttttctgacc aatagatatt catcaaaatg aacattggca
    12721 attgccataa aaacgataaa taacgtattg ggatgttgat taatgatgtg cttgatgcgc
    12781 tgactgttag aagcatcatg gatgaaacag tcctcattaa taaacaccac tgaagggcgc
    12841 tgtgaatcac aagctatggc aaggtcatca acggtttcaa tgtcgttgat ttctcttttt
    12901 ttaacccctc tactcaacag atacccggtt aaacctagtc gggtgtaact acataaatcc
    12961 ataataatcg ttgacatggc ataccctcac tcaatgcgta acgataattc cccttacctg
    13021 aatatttcat catgactaaa cggaacaaca tgggtcacct aatgcgccac tctcgcgatt
    13081 tttcaggcgg acttactatc ccgtaaagtg ttgtataatt tgcctggaat tgtcttaaag
    13141 taaagtaaat gttgcgatat gtgagtgagc ttaaaacaaa tatttcgcca caggagtatc
    13201 ctggaagatg ttcgcaggta ttatttttcg gataatcctt aggataacat gataaacgtt
    13261 acggaattat attaatggca attcactaat aatatcagcc agcaaattaa aaatttcact
    13321 gaataaatgt tcgcaaaaag gcgcaattaa cggcattaat gccgccatta aagagatacc
    13381 gacagtcaga gttaatggaa atccaataac aaaaatggat aattgcgggg ccatacgatt
    13441 cagtaaacct aatgccagat tcagggtcag cagcagagta atgagcggta acgccagcat
    13501 cagcccgtta aggaaaatca aactccccgc tttggtgagt gccagaaacg cattgctgtt
    13561 caacggttcg ccaccaattg gcagggtgtg aaaggtatcg accagcagtg aaatcaacca
    13621 taaatgaccg ttaaatgtca ggaacagcag taacgccagc atatccatga tacgcgctaa
    13681 aacgggcata ttgagatggc tggctggatc gacaaacgtc gcaaatgaca gccccatttg
    13741 cagaccgata atttcgccag cggttcgcac agcggcaaag gcaaattgca tggtaaaacc
    13801 aagcgcaacg ccgatcagga tctgctgcac ggccagccac agagcaaaga acgaaaaaac
    13861 aggaacatcg ttggcaggta atgatggggc aatagcgaac gtgatcatca ttgccagacc
    13921 cagttttacc cgtttcggca cgctgcgttc gctcagaatt ggcgcggtag aaatgagcgc
    13981 cagcacgcgc agtaacggcc agaagtagag gcttaaccag gaaagccatt gatcgcttgt
    14041 cacctgcatc attgtacggg ctacccgatg atatacggca ggttagtgaa caaggtgcgg
    14101 acgtaatcca gcaacagatt gagcatccac ggtccggcaa taataatggc aataaatacg
    14161 gcgatgattt ttggtataaa cgacagcgtc atttcgttga tctgtgtggc ggcctgcaaa
    14221 atactgatga taaggcccgt gaccagcgcc accagcaaca gcggggcagc tagtgccagc
    14281 gcgactttca tcgcttcagt ccccatcatc atgaccgatt caggtgtcat tttgcctctc
    14341 tagctgtaaa agctctgcgc cagcgaaccg accagcaatt gccaaccatc caccagtaca
    14401 aacagcatca gtttaaaggg cagagcaatg gtggcggggg ggaccatcat catccccaat
    14461 gccatcaaca cgctggcaat caccaggtcg ataatcaaaa aagggatgaa aatcgtgaag
    14521 cctatctgaa atgcggtttt caactcgctg gtcacgtagg ccgggagcaa aatgcgcatc
    14581 ggcacggctt caggtccctg caacgggccg gtattcgcca gtctggcaaa caaccctaaa
    14641 tccgcctcac gggtctgacg cagcataaac tcacgcagcg gctgcgcccc tttttccagc
    14701 gcctcctgca ttgatatttt ctcttcgctg aatggctggt acgcatcgac ataaatttta
    14761 tcgatcaccg gcgacataat aaaaaaggtc aaaaacagcg ccagtcccag caatacctgg
    14821 ttaggtggcg cggagggcgt tcccagcgcg ttacgcaata aaccaaaaac aataatgatg
    14881 cgggtgaaac tggtcatcat tagtaaaatt gccggaataa acgtcaacga ggtgatgaac
    14941 accagcgtct gcaccgggag cgaccagctt tgtccaccgc caggcagcgg ctggctggtg
    15001 atacctggca gttgcgcgaa ggcgaggggc gtaaccagcc agagaaggac aggggcgaca
    15061 gacaacaaac gacgcatcag gatctcccgc tacgcttaag caagtttttc atgaccgact
    15121 gaaaatcggt ctgaggtatc tcttccgttg gtgctgaagg gggaagttta tgcagcagat
    15181 tgatttgccc tgcggtaacg ccgagcacca gccgtgcatc ttccacatcg accaccacaa
    15241 cccgttcacg cgcgcccagc gaagcactgg cgctaatttt cagaccatta acgccagtgc
    15301 gtttcggggc aaatcccagc cgttttacca gccaggcagc agcgaggatc agggcaataa
    15361 tggcgatgag tgcgccgctc acctgcagca gtggcgcagc agaaaccggc gcggaagatt
    15421 gcacagtagc gtggttattc atcactaacg gctcaggcgg cgcattcgct cagacggagt
    15481 aatgatatcg gtgatccgca cgccatattt atcggcaacg accaccactt cgccctgggc
    15541 gattaaataa ccgttgatca gaatatccag tggttcgccc gccagaccgt ccagcgccac
    15601 gacggaccct tgcgtcagac gcaacagctc tttgatggtc atccgcgtac ggcccagctc
    15661 gacggtcagc ttgaccggaa tatccattat caggtcgata tcctgcaacg ttccgctgac
    15721 atcaccaccg ccaaattgct ggaacaccgc gtcggcagcg cttttgctgc tggttgattt
    15781 ttgttcgctc aacgcttcag cccacagatc gtccattgcg ccgttgttgt catcggccgg
    15841 attattcatg tcactcattt gggctgttcc tcgttcagag aatttaaaat cgggttaatc
    15901 aaatgttcta tccgtaacgc atactgaccg ttgagagtgc cgtactgact ggtcagcacc
    15961 gggacgccgt caacatgggc gatgatgcga tcgggttttt ctatcggcag gacgtcgccg
    16021 ggtttcagtt ttaaaatctg cgacaggcgt agcgagatat cggcaaagtt ggcgaccagc
    16081 tccagctgtg aatgctgcac ctggcgcacc aggttatcgc gccagttctg atcttcatta
    16141 cgcgagtttt ccagcggcgg gttaaccaac aattcccgta gcggctcgat catgctgaat
    16201 ggcagacaga tattaaattc gccggtcagg ttgccaatct ccacatggaa cggcgtgtta
    16261 accacaatgt cgttcggcga ggtggtgata ttggtaaatt tcacctgcat ttccgaacgc
    16321 acgtactcaa cttccagcgg attaatcgcc ttccaggcgt cgctatagcc ttcaagcgcc
    16381 agctttaaca tgcggttgat gacgcgctgt tcggtatggg taaactcgcg accttccact
    16441 ttggtcggga agcgtccatc gccgccaaac aggttatcca cggcgataaa caccagactc
    16501 ggtgaaaaca ccaccagccc tgtgccgcgc agcggtttca gatggataag gttcaggttg
    16561 gtcggcaccg gcaggttgcg ggcaaattca tggtacggct gaatgcggat ggccccgacg
    16621 gttatatccg ggctacgacg cagcaggttg aacagcccca tacgaaaatg gcgggcaaag
    16681 cgctcattaa tgatttccag cgcctgcaaa cgttcgcgca caacccgtcg ttgggtattc
    16741 ggatcgtacg gacgaatgtc actttcgccg ctaacactgg ctgtcggttc gtctttgact
    16801 tcgctgtcac cattcaacag cgcatcaatt tcagcttgag aaagaatact atcgcccatg
    16861 tcgttatcgc agaataaaag cggtatacag cacgtcggtg acatcctgtt tcggttgccc
    16921 ggcaacaagc ggggtggaaa gcgtggtttt aatctcggca atcaggtttt tcttgccttc
    16981 ttctgtcgcc agtacggcag catcctgacg cgaaaacagt aacagcaaac ggctacggac
    17041 ttccggcaaa tactcactca gccgcgagcg ggtagcttca tctttcaggc gcagggttat
    17101 gccgatataa agtacgcgat ctgcatcgcc cagattgacc gtaaaggtat ccagcgcata
    17161 gaagaccggg gagggcacga cgcgttgctg cgctttgtcg tcagcggcaa cctgatgcga
    17221 atgccagtag ctgtaacctg cgctggcaca ggccgcgagg gtaatgaata ccagaatcgg
    17281 gatccaaagc gatcgcttgc ttttcttgct tatcgcgtaa tcagtcatgt gttgcggtct
    17341 tcctgtgtcg ctactgctta tcggctaatt atcctgcgtc ttgagcgcaa caaagcgggg
    17401 aaaagacgcg gattacggtg ctacctctga cgttaggcga aaatatcaac gccgctgttg
    17461 cctgttacac gtacttgtaa agagacggga acctgaagcg tatcgtcgtc ttcccccgcc
    17521 agaggttcat ggtttgctgt gcgttggctt tgctgttgct gggaagcggc ctgctgctga
    17581 ccactaaagc tttcgccact gatgttgctt tgccctaact gaatgccact ttcggccagc
    17641 tgcgttcgca gtaccggaag cgctgcttcc agggcggcgc gtacgtgttg atgcggtgaa
    17701 accatctgga tttgcgcctg gttatcatcc actttgaggg agatttgcac ttcacctaaa
    17761 tcctgcgggt gcagacgcaa ctctgcactt tgttgcccct ggcgggtgaa cagcgaaata
    17821 tgctggctta atgattgttg ccattcgtga gaacccagcg gtgcactcaa cacaggcgcg
    17881 gcgacggtgg gcagtggctg tgtctggtgt ggagtgatta gcgggctggc ggcagcggtc
    17941 accggtgaag gtgtgctgat gacttccgct ttactctggg cttctgctac cagcggtgtt
    18001 aatggctgag ctggcgtgcc gggggcatca tcaggctgtg ctgttgtgag ttgcgcagaa
    18061 gtcagttttg tgaagagcgt tggtttctct gccggtaaca cggttgacgg cacatcagtc
    18121 actttgggcg tattgtcaaa acccggcaac atcgcaaaaa gggcgctcag gcttgcggtg
    18181 acgtcttcat tcagatcatc cgctttttcg tcttttgccg tattgttgcc agccaccgca
    18241 gccaacgtca tcgtttgagc ggtggttaat ggtgttgatg tggattgttc gtcgttgatg
    18301 acaggcggtg tttcatccac agggatcagt aaatccgctt gttgcgcatc ggcaagaatc
    18361 tcgctgacca gcggctcgcc ttttgtcgtg ggcttatctg ttgccaccag caactggggg
    18421 gccgctttgt cggtagttgt ctcgcctgct aatgcttcgc tcaacaacgc gagaaaatct
    18481 tgtgcagcat cgctggcttt gccgccaggc aatgtggtgg tgtcaacgtc ggcggtaatt
    18541 aagggcgcta agcgaatcat tcaggtttcc tcatggcggc gcgctgggcg aactcatcca
    18601 tctttttctg atcgaggcgg ttttctgcaa gcagtgccgc cgtggattgc cgttcctgca
    18661 gtgtctgcca ggcctgcaaa cgttgtttct tttctcgcca gctgttcagg gcaatgtcaa
    18721 ctttctgcgt ccactgatta agttgctggc gatgctgagt aatggctttt tccagcgtct
    18781 ggataaactg ctgatagttg atccagcggt tgctggtcat cccggcactc atatcgctgt
    18841 tgaggttatt gcgatattca ttctgataat cgatcagcat tttgagctgt tcttccgcct
    18901 gctgacaccc gcgacgcatt tcacccagca ggcgcgcggc atcctctacc tctttttctg
    18961 ccagatcttt cagggtcgcc agcgcgccat gttctgccat ctgccgttat ctcctgggtt
    19021 atgacactgt cgggaaaata cgctccagcc cctggagaga cgcttcccag tccgcgcgtt
    19081 caaaaatgcc ttgttgcaaa tagccctcca gctgcggcca cagggcgatg gctttgtcga
    19141 gcatcggatc gctgcctttg gcatacgcgc cgacgctaac cagatcgcgg ttacgctgaa
    19201 aactcgacaa cagctgtttg aaggtgcgca ctcgcgcgta atgttgctca ctgatcaacg
    19261 ccgtcattgc gcggctgatc gacgcttcaa tatcgatagc cggatagtgc ccggcttccg
    19321 ccagtcggcg agacagcaca atgtgaccgt cgaggattgc ccgcgcagag tcggcaatgg
    19381 ggtcctgctg gtcatcgcct tcagtgagca cggtataaaa tgcagtaatc gatccgccgc
    19441 cgctaatgcc atttccggca cgctcgacca gtgccggtaa tttggcaaac accgacgggg
    19501 gataaccttt agtggcgggt ggttcgccaa tcgccagcgc aatttcacgc tgggccatcg
    19561 cgtagcgggt gagggagtcc ataatcagca acacatgctg accacgatcg cgaaaatctt
    19621 cggcaatgcg cgtggcatag gcggcaccct gcattcgcag gagaggagaa acatccgccg
    19681 gagcggcaat caccacggag cgtgcacgcc cttcggcacc gaggatattc tcaataaaat
    19741 cttttacttc gcgtccgcgt tcaccaatca aacccacgac aatgacatcg gcgcgggtgt
    19801 aacgtgccat catccccagc agcacacttt taccaacgcc ggacccggca aacagcccca
    19861 tacgctgccc acgcccaacg gtaagcaggg cattgattgg gcgcacgccg gtgtccagca
    19921 catgttcaat cggtgtacgt tgcaacgggt taaatggcgg ggtaatcagc gcaccggttt
    19981 ccgtcgtatc gggggagggg aggccatcga gcggtttacc gctgccgtcc agaacacgac
    20041 ctaataacgc cggaccgagc ggtaactgct tgccgctttg cagtccttca gccgaaatgt
    20101 ttttggcata aacccgcgcg ccgggcagga caccttccac ttcctccagc ggcattaaaa
    20161 acagccgttg accgttaaag ccaacgactt cgctttctac ttcgtgcgtt tcgctgccgt
    20221 tctggcgttc aatgacacag gttgcgccga gcggcaattg taatccggtg gcttccagca
    20281 ccagcccggt agcgcgggtt aatcgcccgt agcgacgtac cgcaggcaac tgtgccattt
    20341 tggcttcaaa gttatccagc gtggttagcc agcgagtcag gcgcgtggtc attacaccac
    20401 tcctggtgct gccagacggc agagttcttg ccagcgagtg gcgacactgg cgtcgagatc
    20461 gccttcatcg gcggagactt tacagccgcc aggatggagt gtgggatcgc cccgcaagcg
    20521 ccagccatgc aaacttaagg ttgcgccgag catatcatcc acacgttgca gatcatcggg
    20581 gtgcacgcgt agttgcggtt tgccgctgaa taacggttct tgctgcaaca actgttggat
    20641 ctgtttgatc agtgccgagt tatccactgt tggcgtctga ccgatgacct gacgtgccgc
    20701 ctccagcgcc atctgcatca ggcgcgaagc aatcacacta tcaagtgcat caagggtagt
    20761 ttgaaattcg ctgaccagtt gctgcatccg ggcatgaatt ggcgcttgtt gagacttcgc
    20821 ctctgccaga ccttgctcca gcccctgggc cagtccttcc tgatagccct gcttatgacc
    20881 ttgctggcga ccttcggcaa tccccgcctg ataaccttgc tcatgggcct gcatttgcag
    20941 ttgcgccagt tgctgctcaa ggctgggttc ggcctcttca atgatggttt cttccggctc
    21001 gaccatgggc acaaactctg cctgtggtgg cgcgagatcg tccggcgtcc aggttttcca
    21061 cggcagatta tcagacatag gtatcctcgc cgctgccaat taccatctcg ccagtttcgg
    21121 caaggcggcg cacaatcagc agaatcgctt tctgttcgtt ttccacctgc gacagacgca
    21181 ccggaccacg gttggcgaga tcgtcgcgca gaatatcggc ggcacgctgc gacatattgc
    21241 gcaggaattt ctcgcgcagt ggctgctcgg cccctttcag cgcgatcaac agcgattcgg
    21301 aatccacttc ctgcaacaga cgctgaatgc tgcgatcgtc gacatccacc agattttcga
    21361 acaggaacat ctcgtcgata attttttgcg ccagctcgcc gtcgaattcg cgcacggcgg
    21421 taataacggc ttcttcctgc tgagttttca tcaggttgat aatttcggct gccgttctca
    21481 cgccgcccat tttgctgcgc ttgagattct gaccgtcgag caagccattc agtacttcgg
    21541 tcagttccgc cagcgcggct ggctgcacgc cgccaaaggt ggcgatacgc aacatcacgt
    21601 cgtggcgcag acgttcatcg aacaacgcca gaatatcggc ggcctgcgcg cgcttcagat
    21661 gcaccagaat ggtggcgata atttgcggat gctcatcgcg aatcagatcg gcggcactct
    21721 gcggctccat aaagttgagc gtttcaatac cgctggcggt atcgcgagtt tcgagaatat
    21781 cttccagcag gctggcggca cgttcctcac ccagagcttt gaccaatacc gaacgcagat
    21841 aatcgttggc gttgatattc agtgcggcaa actgttcagc ttcttgctca aactccgcca
    21901 gcacatcggt tagctgcttg ttggagatct gcgtgacgtt cgccattgca gcgctcaggg
    21961 tttgcacttc gcgctgggag aggtgcttga acacctctgc cgcccggtct tcgccaatgg
    22021 tcatcagcag gatgacgctt ttatcggtgc ctgtcaggtt actcatgatc gttatttatc
    22081 cactggcgaa tgaccagcgc caccacgcgc ggatcgttat cagacatttc acggatacgc
    22141 tggctcatga cttctgcccc cagacgttgg ttagcgcgtc gttgttggag ttgttcgtct
    22201 ttgctcaggc ggacttccac cgcatcttcc acttcctcac gggcctgcgc ctgttgctgt
    22261 acagctttca tcgcctcggc gcgacgtgtt aactgcgggc gtaccgcttt ccgccacagc
    22321 agccacgcca ccagcagtac cagcaaccag cgaccggcag ccagcaactg atcgataaac
    22381 gcttgctgtt gccagaacgg cagttctccg ccgctttcgt cactgctatt gaacggcgag
    22441 ttaacgacat tgagcgagtc accgcgtttt tcagaaaagc ccatcgcctc gcgggtcaga
    22501 tcttcaattt gcttcatctg ttcgttgctg agaggcaatg gtttaccgtc tggcaaggtt
    22561 ttgtaattca ccactaccgc gactgacaga cgttgcacat cgcccacgtt cattttggta
    22621 tgacgaatgg tgcgatcgac ttcgtagtta ctggtttcat tccgctgtgt gctacgcggc
    22681 ccgctattgc tggtggtgct cgcctgctgc tggcggttat tttgatttgc cggaggcgtg
    22741 ctgattggcg cgttattcgc aggtgccggt tgattcgaca acgcccccgg tacgccgccc
    22801 ggataaccgg aaccgctttg ctcgctctca ttaagctggc gtgaacgaag cgccgcatga
    22861 gattcatcac cgttagggcg atactgttct tccgtttgtt ctttactggc gaagtccagt
    22921 tgcgccgtga cctgggcgtg aatattaccg ttaccaacaa ttggcgacag gatcgcttca
    22981 atacgccgct gaatccggcc ttcgacatcg ctggcatatt tcaactgagc gtcattaaga
    23041 tcgcgcccgc tggtattgga ctgggttaac agatgtccac cctgatccac cagcgtgacg
    23101 ttccccggcg gcagaccagc aaccgcgctg gaaaccagat gtacaatggc gctaatttgc
    23161 ccttcatcga gtgcgcggcc gggtaacaga tttaccgtca ccgatgcaga aggggatttt
    23221 tgttcacgga cgaataaaga cggtttcggc attgccagat gtacgcgcgc cccttttacc
    23281 gggccgatag tttcgatggt acgagaaagc tcgccttcca gcgcccgctg ataattcacc
    23341 tgttcgctga actggctgat accaaacttt tcctgatcga gtagttcgaa accgaccgcg
    23401 ccgcctttgg gcaaaccttg ttgcgccagg cgcagacgca gctcgtgaac tttatctgcc
    23461 ggaacttcaa tagcgccgct ggcttcgctg aagcggtaag gaatattcat ttgcgtcagt
    23521 tggctgacaa ttgcgccacc atcctgatcg gaaagattgc tgaataatgt gcggtagtcg
    23581 ggggctttcg cccacaggat cagtgcgacc atgaccgcca ctgccgcgga accggcaaca
    23641 atcaatggaa ttttcggatt cgcacgcagg cgattaagcc actcaagaga ttttgtctgg
    23701 gctgcagtcg cattcatcgc gcacctcgtg gctgattatg gacggcgtta ttggcaaagt
    23761 ggaacaaaac tcactcccgg gtcagcaaac tcgaaaaatt gacgctgcca ttatttgtgc
    23821 tttcgttttt ttctatccgc caataaaccc gtttttttgt tgctatttag cgcctttgtc
    23881 ttattgactt actggtaggc tttgctacca gaaattatcc aggagacgaa aatgtcagcg
    23941 atacagggga ttgaaggggt tatcagcctg ttacaggcta cggcgatgag tgcgcgtgcg
    24001 caggattcac tgccgcaacc gaccattagt tttgccgggc agctgcacgc cgcgctcgat
    24061 cgcattagcg atacacaaac agctgcccgc acgcaggcag aaaaattcac cctcggtgaa
    24121 cccggcgtgg cgttaaacga tgtgatgacc gatatgcaaa aagcctcagt ttctatgcaa
    24181 atggggattc aggtgcgtaa taagctggtg gcggcgtatc aggaagtgat gtccatgcag
    24241 gtgtaggttt tgtaacctgt tgttaattac atcccatctg cccgcgtggg gcatggatgg
    24301 ggcaaactca cttaatttct ggtttaaaat ggatacctga tcctgaaagt tttctgccat
    24361 ccacgatccg taaacgcgat aatatgtttt gtcttcgctt tgctcatagg aagagtgaac
    24421 tcttttgtta gcctggggtt ccttctgatc attattgttc ctgcctttat ctcttgccac
    24481 gccagagccc cctggataca catacaccag gatgaaaacg gtgagttgtg ttcaaactgt
    24541 agtacaattc tctccagtat gaacaggaaa gaatatgcta tgaaccctta tatttatctt
    24601 ggtggcgcaa tacttgcaga ggtcattggt acaaccttaa tgaagttttc agaggggttt
    24661 acacggttat ggccctctgt tggcacaatt atttgctatt gtgcatcatt ttggttatta
    24721 gctcagacac tggcttatat acctacaggg attgcttatg ctatctggtc aggagtcggt
    24781 atcgttctga ttagtttgct ttcctgggga ttttttggcc aacgactgga cttgccagcc
    24841 atcataggca tgatgttgat ttgtgccggt gtgttagtca ttaacttgtt gtcacgaagc
    24901 gcaccgcatt aaaaaataat catccttgag ataacaggaa tctgatttgt gcatattatg
    24961 gctaatgttc ggggcaactt tataattatg attgcatttg cgcgatcatt gcgccttttt
    25021 gcataatgag atcaatgcac atcaaataaa ataacgatag gtcacaacaa agaggttttt
    25081 atgaaaaaac ttatcgtttc aagtgtattg gcattcataa cattttctgc gcaggccgca
    25141 gcatttcagg tcactagtaa tgaaataaaa acaggagagc aacttacaac gtctcatgta
    25201 ttttctggat ttgggtgtga ggggggtaat acatcgccct cattaacctg gtctggtgct
    25261 ccagaaggca ccaaaagctt tgccgtaact gtgtatgatc cagatgcgcc tacaggcagc
    25321 ggatggtggc actggactgt tgctaatatt ccagcaacgg taacatattt gcccgctgat
    25381 gcaggaagac gtgatggtac aaaacttccg actggtgctg tccaaggccg aaatgatttt
    25441 ggctatgcgg gatttggtgg agcatgtcct cccaaaggag ataaacccca tcattaccag
    25501 tttaaaatat gggctctaaa aactgataag attcctgtgg attctaattc cagcggagcg
    25561 ttagttggtt atatgcttaa tgccaataaa attgcaaccg ctgagataat acccgtttat
    25621 gaggtaaagt aggataagag tatgctggca aaagatagga ctaacctaaa gattgaagaa
    25681 atacggatgc ataaacatca tgagattcac agggttaagc ctcttatgcc agctttatgt
    25741 cgtatccgtc agggaaaaaa agttatcaat tgggagacgc atagtctaac tgttgataat
    25801 aatcagataa tattatttcc ctgtggatat gaattttata ttgcgaatta cccagaagca
    25861 ggtctttatc ttgcagaaat gctttattat cccattgatc taattgaaaa gttccaaaat
    25921 ctttatgcga taaccgatca aattcgtaac acgacaggtt tctgcttacc tcagaatccc
    25981 gagttaatat attgttggga
//
LOCUS       DAOFQD010000066        25662 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.066, whole genome shotgun sequence.
ACCESSION   DAOFQD010000066 DAOFQD010000000
VERSION     DAOFQD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.066"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            139..822
                     /locus_tag="QBF46_002760"
     CDS             139..822
                     /locus_tag="QBF46_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDQ6674921.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGVEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            866..5890
                     /locus_tag="QBF46_002761"
     CDS             866..5890
                     /locus_tag="QBF46_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDQ6674922.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGALADNYDGQGVDYGDGSASDGWVAIGKGAKANIFLNNAGASTALGYDAIAEG
                     QYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSFGQYSMALGRYSKALGRL
                     SIAMGDSSKADGANAIALGNAAKAAGIMSIGLGDNANASQDYAMALGAESEAAENATA
                     IGNKAHAKGVNSIALGNGSQALADSAIAIGQGNKANGADAIALGNGSQSSGLNAIALG
                     KASVVTGDNSLALGSNTNANGINSVALGAGSIADQDDSVSVGSDSLQRKIVNVKNGTI
                     KADSHDAINGSQLYAISDSVAKRLGGGSSVNVDDGTVKAPTYNLKNGNKNNVGDALTV
                     LDQFTLQWDQNRDKYSAAHGSSTASVITDVADGAVSDSSKDAVNGSQLKATNDDVETN
                     TTNIATNTGNIATNTANIATNTTNITNLTDTVGDLKDDALLWNGTAFNAAHGTETTST
                     ITNVKAGTLSDDSTDAVNGSQLKDTNDNVATNTTNIASNTANIATNTSNIADNTANIA
                     TNTSNIADNTANIATNTSNIAGNTANIATNTTNIAANTTSINSLNTSVDALEQDAMLW
                     NGTAFNAAHGTETTSTITNVKAGTLSDDSTDAVNGSQLKATNDNVATNTTNIASNTAN
                     IATNTANINTLNTSIDTLEQDAILWNGTAYSAAHGTETASTITNVKAGTLSENSTDAV
                     NGAQLNATNANVATNTTNIATNTASINTLNTSIDALEQDALLWDGTAFSAAHGANKDA
                     SKITNVLAGTVSSASTDAINGSQLHGLSSSIATYLGGGATVSDSGVFSGPTYNIDGND
                     YTNVGAALDAINTSLSDSLGDALLWDSTTGAFSAKHGSTASVITNVADGAVSDSSSDA
                     VNGSQLYDVSNSVVDVLGGGAGVNTDGSISAPTYTIANTDYDNVGDALNALDTTLDDA
                     MLWDATAGENGAFSASHDGSASKITNVAAGTISDTSTDAVNGAQLHGVSSSVAEALGG
                     GAAVNSDGSISAPTYTIADTDYTNVGDAMNAIDSTLDNALLWDATAGENGAFNASHDG
                     KASVITNVANGQINETSTDAVNGSQLNATNMLIQNIAGDTSESYITENGEGINYVRTN
                     DSGLVFEDASATGVGATAVGYNSVASGDSSVAIGQNSSSTIESGIALGSSSVSNRVIL
                     QGSRDTSVTEDGVVIGYNTSDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPSKYFHANSTEEDSLAVGEDSLAMGAKTIVNGDAGIGIGLNTLVLTDAI
                     NGIAIGSNASANHANSIAMGSGSQTTRGAQTDYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVADEENTISVGSS
                     TNQRRITNVAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6317..7972
                     /gene="lldP"
                     /locus_tag="QBF46_002762"
     CDS             6317..7972
                     /gene="lldP"
                     /locus_tag="QBF46_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDQ6674923.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSVVWLKMKPS
                     DSISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7972..8748
                     /gene="lldR"
                     /locus_tag="QBF46_002763"
     CDS             7972..8748
                     /gene="lldR"
                     /locus_tag="QBF46_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDQ6674924.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8745..9935
                     /gene="lldD"
                     /locus_tag="QBF46_002764"
     CDS             8745..9935
                     /gene="lldD"
                     /locus_tag="QBF46_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDQ6674925.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10121..10594
                     /gene="trmL"
                     /locus_tag="QBF46_002765"
     CDS             10121..10594
                     /gene="trmL"
                     /locus_tag="QBF46_002765"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDQ6674926.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10647..11468)
                     /gene="cysE"
                     /locus_tag="QBF46_002766"
     CDS             complement(10647..11468)
                     /gene="cysE"
                     /locus_tag="QBF46_002766"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDQ6674927.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11548..12567)
                     /gene="gpsA"
                     /locus_tag="QBF46_002767"
     CDS             complement(11548..12567)
                     /gene="gpsA"
                     /locus_tag="QBF46_002767"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDQ6674928.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12567..13034)
                     /gene="secB"
                     /locus_tag="QBF46_002768"
     CDS             complement(12567..13034)
                     /gene="secB"
                     /locus_tag="QBF46_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDQ6674929.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(13097..13348)
                     /gene="grxC"
                     /locus_tag="QBF46_002769"
     CDS             complement(13097..13348)
                     /gene="grxC"
                     /locus_tag="QBF46_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDQ6674930.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIGAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13490..13921)
                     /locus_tag="QBF46_002770"
     CDS             complement(13490..13921)
                     /locus_tag="QBF46_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDQ6674931.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKVNNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14166..15710
                     /gene="gpmM"
                     /locus_tag="QBF46_002771"
                     /gene_synonym="pgmI"
     CDS             14166..15710
                     /gene="gpmM"
                     /locus_tag="QBF46_002771"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDQ6674932.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15720..17003
                     /gene="envC"
                     /locus_tag="QBF46_002772"
     CDS             15720..17003
                     /gene="envC"
                     /locus_tag="QBF46_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDQ6674933.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            17007..17966
                     /locus_tag="QBF46_002773"
     CDS             17007..17966
                     /locus_tag="QBF46_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDQ6674934.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17953..18987)
                     /gene="waaH"
                     /locus_tag="QBF46_002774"
     CDS             complement(17953..18987)
                     /gene="waaH"
                     /locus_tag="QBF46_002774"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDQ6674935.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKIMIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19226..20251)
                     /gene="tdh"
                     /locus_tag="QBF46_002775"
     CDS             complement(19226..20251)
                     /gene="tdh"
                     /locus_tag="QBF46_002775"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDQ6674936.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHIGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            complement(20261..21457)
                     /gene="kbl"
                     /locus_tag="QBF46_002776"
     CDS             complement(20261..21457)
                     /gene="kbl"
                     /locus_tag="QBF46_002776"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDQ6674937.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            21671..22603
                     /gene="rfaD"
                     /locus_tag="QBF46_002777"
     CDS             21671..22603
                     /gene="rfaD"
                     /locus_tag="QBF46_002777"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDQ6674938.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKSITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNKGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVMEYMAWLNRDA"
     gene            22613..23659
                     /gene="rfaF"
                     /locus_tag="QBF46_002778"
     CDS             22613..23659
                     /gene="rfaF"
                     /locus_tag="QBF46_002778"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDQ6674939.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23663..24634
                     /gene="rfaC"
                     /locus_tag="QBF46_002779"
     CDS             23663..24634
                     /gene="rfaC"
                     /locus_tag="QBF46_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDQ6674940.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(24704..>25662)
                     /locus_tag="QBF46_002780"
     CDS             complement(24704..>25662)
                     /locus_tag="QBF46_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDQ6674941.1"
                     /translation="TGKIALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFA
                     HAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILILKSDLRHKTTLYTINFMLS
                     LCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLYCFITIALLAGVFSFKDTLL
                     MRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEP
                     RLSGALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFS
                     LLVVGLSDVILFSKPVPTAVFITIILLCAYFKAQSDQCLLEK"
BASE COUNT         6528 a         6565 c         6522 g         6047 t
ORIGIN      
        1 tttttatgat gaatgcttaa aacaatattt aatcgacaca catcactgtc attaatgtaa
       61 agcaacatag ctcaccaata atacaatgga caactctacc ggcattgatc gctggtcgga
      121 ggaaataagg atttttacat gaacttgaag aagatttttt tcagtgctgt cactgtttca
      181 gttctctgcg cattaacggg atgtgactac attgaagaag gcaagccaga aagtagttta
      241 ctgaaacaac aagaagaaca taataataaa attgtgctac ttgaaaaaca acaagcacag
      301 ctaaagagtc aactggaaac tattcaaaaa caacaaactg gcataataag cagtacaaag
      361 actttaactc acgtaattaa atccgttaaa gatcaacaaa acacttttat ttttaccgag
      421 tttaacccag caaaaacgaa gtatttcatc ctgaacaatg gttcggtggc tttagcgggt
      481 cgagtgttat ccattgacgc cacagagaat ggcagtgtta ttcatatttc actggtcaac
      541 ttattaagta cacctatctc aaatattggt tttaatgcga catggggtgt tgaaaaacct
      601 gtcgatgcca aagagtttgc ccggtggcaa caattacttt tcaacacatc aatgaaatcc
      661 acattgaaat tattaccagg tcaatggcaa gacattaatt tgaccctgaa gggtgtatcg
      721 cccaataacc tgggatatct gaagttagcc atcaacatgg aaaatattca gttcgacaat
      781 cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat aaatgcagtt agtacgctta
      841 taacaaggaa tagcgagcat cattaatgaa caaaatattt aaagttatct ggaaccctgc
      901 gacagggaat tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga aatctgggcg
      961 cagtaagctg ttaatttctg cgctggttgc aggtgggttg ttgtcgtcgt ttggggcgct
     1021 ggctgataat tacgacggtc agggcgttga ttacggcgat ggctcagcta gtgacggctg
     1081 ggttgctatc ggtaaaggtg caaaagcaaa tattttttta aacaacgctg gtgccagtac
     1141 cgctttaggt tatgacgcta tagctgaagg ccaatatagc tctgccatcg gctcaaaaac
     1201 ccatgctatt ggtggtgcat caatggcctt tggggttagt gcaatctctg aaggtgacag
     1261 gagtatcgcg ctgggtgcat cgtcgtattc attcggccaa tactcaatgg cccttggtcg
     1321 ttattccaaa gcgctgggta gattgtctat agctatgggg gatagctcca aagcggatgg
     1381 agcaaacgcc attgcgctgg gaaatgccgc taaggcggct ggtattatga gcatcggtct
     1441 cggtgataat gccaatgcgt cacaagatta tgctatggcg ctgggagcag aaagcgaagc
     1501 cgctgaaaat gcgaccgcta tcggcaataa agcgcatgca aaaggagtga atagcatcgc
     1561 gttgggtaat ggcagccagg ctctggcaga tagtgcaatt gctattggcc agggcaacaa
     1621 ggctaacggc gctgatgcta tcgctctggg taatggtagc cagtcgagtg gcttaaacgc
     1681 tattgcctta ggtaaggcca gtgttgtaac tggcgataat agtcttgcgc ttgggagcaa
     1741 taccaatgcc aacggtatta actctgtcgc gctgggcgca ggttctattg cggatcaaga
     1801 cgatagcgtt tctgtcggca gtgactcatt acaacgcaag atcgttaatg taaaaaatgg
     1861 cacgatcaag gctgatagcc acgacgccat taatggctca cagctttatg ccatcagcga
     1921 ctcggtggca aaacggcttg gcgggggttc atcggtaaac gttgacgacg gtactgttaa
     1981 agcaccaacc tacaatttaa aaaatggtaa caaaaataac gttggcgatg cgctcactgt
     2041 actcgatcaa ttcaccctgc aatgggatca aaacagagac aaatacagcg ccgctcatgg
     2101 tagctcaact gccagcgtaa tcaccgatgt ggcggatggc gcagtttcag attccagtaa
     2161 agacgcggtt aacggttcac aactgaaagc caccaatgat gatgttgaga ccaataccac
     2221 caatatcgct accaataccg gaaatattgc aactaacact gcaaatattg ctaccaacac
     2281 caccaatatc actaatttga cagatactgt tggcgacctt aaagatgatg ccctgctctg
     2341 gaatggcact gcattcaacg ccgctcatgg tacggaaacc accagcacca tcaccaacgt
     2401 gaaagctggt accctttcgg atgacagtac tgacgcggtt aatggctcgc aactgaaaga
     2461 cactaacgat aacgtggcga ccaacaccac caatatcgct agcaacacgg ctaacattgc
     2521 cactaacacc agtaatattg ccgataacac tgccaacatt gctaccaaca ccagtaatat
     2581 tgccgataac actgccaaca ttgcgaccaa caccagcaat attgcaggta acactgccaa
     2641 cattgctacc aataccacta atatcgcggc taacaccaca agcataaata gcctgaacac
     2701 gtctgtggat gctcttgaac aggatgctat gctctggaac ggcactgcat tcaacgccgc
     2761 tcatggtacg gaaaccacca gcaccatcac taatgtgaaa gctggcaccc tttcggatga
     2821 cagtactgac gcggttaatg gctcgcaact gaaagctact aacgataacg tggcaaccaa
     2881 caccaccaat atcgccagca atacggctaa cattgcgacc aacaccgcca atattaatac
     2941 tctcaatacg tcgatcgata cccttgagca ggatgcaata ctctggaacg gaacagcgta
     3001 tagcgccgcg cacggtacag aaacagccag cactattacc aacgttaaag caggtacttt
     3061 atctgaaaat agtacggacg cagttaacgg tgcgcagctg aacgcgacta acgcgaatgt
     3121 ggcgaccaac accaccaata ttgctactaa caccgccagc attaatactc tgaatacatc
     3181 cattgacgct ctggaacagg atgcgctgct ttgggatggc actgcattca gtgcagcgca
     3241 tggtgcaaac aaagatgcca gcaaaatcac caatgtcctg gcagggactg tatcatccgc
     3301 cagtactgat gccattaatg gcagccagct tcatgggttg agcagttcga tcgccaccta
     3361 tcttggtggt ggtgccactg tgagcgattc tggcgtattt agcggcccta cctataacat
     3421 tgatggtaat gattacacca atgttggtgc tgcacttgac gccattaaca cctcacttag
     3481 cgactcactc ggcgatgccc tcctttggga tagcacgact ggcgcattta gtgccaaaca
     3541 cggttctacc gccagcgtaa tcactaacgt cgcagacggt gcagtctctg actctagctc
     3601 tgatgctgtg aatggttcac aactgtacga tgtaagcaac tctgttgtcg atgttctggg
     3661 tggtggtgct ggcgtgaata cggatggcag catcagtgca ccaacgtaca ccattgctaa
     3721 cactgattac gataatgtcg gcgatgccct aaacgcgctt gataccactc ttgacgatgc
     3781 gatgctatgg gatgctaccg caggtgaaaa tggtgccttc agtgccagcc acgatggcag
     3841 tgccagcaaa ataactaacg tcgcggcagg aacaatttct gacaccagca ccgatgcggt
     3901 taatggggct caactccacg gcgttagcag ttccgtcgct gaggctcttg gtggtggtgc
     3961 ggcggtgaat tctgacggca gcatcagcgc accgacttac accattgctg ataccgacta
     4021 taccaacgtc ggcgatgcga tgaatgcaat tgactcaacc ctggataacg ccttgctttg
     4081 ggacgctacc gcaggtgaaa acggtgcgtt taacgccagc catgatggca aagccagtgt
     4141 catcaccaac gtagccaatg gtcagattaa cgaaaccagc accgacgcgg ttaacggttc
     4201 ccagttaaat gccacgaata tgttgatcca gaatattgcc ggtgatacca gcgaaagcta
     4261 tatcacagag aacggtgaag gtatcaacta tgtgcgtact aacgacagcg gcttagtctt
     4321 tgaggatgcc agtgctacgg gtgtgggcgc tacagctgta ggttataact ccgtcgcatc
     4381 aggcgatagc agtgtggcaa ttggacagaa cagcagcagt actattgaat ctggaattgc
     4441 tctgggcagc agttctgtgt ctaaccgtgt catccttcag ggctcccgtg ataccagcgt
     4501 aaccgaagat ggcgtagtca ttggttataa caccagtgat ggcgaactgc ttggcgcgtt
     4561 gtcaattggt gatgacggta aatatcgtca aatcatcaac gtcgccgatg gttccgaagc
     4621 ccatgacgcc gttacggttc gccagttgca gaatgcgatt ggtgctgtag caacgacacc
     4681 gtccaaatac ttccatgcta attcaacgga agaagattca ctggctgtcg gtgaagactc
     4741 actggcaatg ggtgcgaaaa ccattgttaa tggtgatgcc gggatcggta ttggcctgaa
     4801 cactctggtg ttaactgatg caatcaacgg tattgctatc ggtagcaacg cgagtgcaaa
     4861 tcatgcaaac agtattgcga tgggtagtgg ttcccagacc acccgtggtg cgcagactga
     4921 ctacaccgcc tacaacatgg acgcgccgca gaattctgtc ggtgaattct ctgtcggcag
     4981 cgaagacggt caacgtcaga tcaccaacgt cgcggctggt tcagcggata ccgatgcggt
     5041 taacgtaggt cagttgaaag tcactgatga gcgcgtagcg caaaataccc agagcattac
     5101 taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat
     5161 tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga
     5221 tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga
     5281 caacagcgtc gcactgggta ccggttccgt tgcagatgaa gaaaatacaa tctctgtagg
     5341 ttcttccact aaccaacgcc gtattactaa cgttgccgca ggtaaaaatg ctaccgatgc
     5401 tgttaacgtt gcgcagttga agtcttctga ggcgggcggc gtgcgttacg acaccaaagc
     5461 tgatggttct atcgactata gcaatatcac cctcggtggc ggcaacggtg gtacgactcg
     5521 tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa
     5581 gcaaagcgtg caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa
     5641 actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg
     5701 tctgccgcag gcttacactc caggtgccag catggcttct attggtggcg gtacttacaa
     5761 cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta
     5821 caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat
     5881 tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc
     5941 gctacgctta tcaggcctac agggtgattc tgcacattcc tataggccga gtaaggtgtt
     6001 cacgccgcat ctggcaagat aaggcgctcc gggtcaacaa tctaagggca attctctgat
     6061 gaggattgcc cttttcttta ccagacatct ccccccacaa gaattggccc taccaattct
     6121 tcgcttatct gacctctggt tcacaattcc ccaattaaaa ctcacatcaa tattgccaat
     6181 acataacatt tagttaacca ttcattgtca ttatccctac acaacacaat tggcagtgcc
     6241 acttttacac aacgtgtgac aaggagatga gcaacagact cattacacga tgtgcgtgga
     6301 ctccaggaga cctgcaatga atctctggca acaaaactac gatcccgccg ggaatatctg
     6361 gctttccagt ctgatagcat cgcttcccat cctgtttttc ttctttgcgc tgattaagct
     6421 caaactgaaa ggatacgtcg ccgcctcatg gacggtagca atcgcccttg ccgtggcttt
     6481 gctgttctat aaaatgccgg tcgctaacgc gctggcctcg gtggtttatg gcttcttcta
     6541 tggtttgtgg cccatcgcgt ggatcattat tgcagcagtg ttcgtctata agatctcggt
     6601 gaaaaccggg cagtttgaca tcattcgctc gtctattctt tcgataaccc ctgaccagcg
     6661 tctgcaaatg ctgatcgtcg gtttctgttt cggcgctttc cttgaaggag ccgcaggctt
     6721 tggcgcaccg gtagcaatta ccgccgcatt gctggtcggc ctgggtttta aaccgctgta
     6781 cgccgccgga ctgtgcctga ttgttaacac cgcgccagtg gcatttggcg cgatgggcat
     6841 tccaatcctg gttgccggac aagtaacagg catcgacagt tttgagattg gccagatggt
     6901 ggggcgtcag ctaccgttta tgaccattat cgtgctgttc tggatcatgg cgattatgga
     6961 cggctggcgc ggtatcaaag agacgtggcc tgcggtcgtg gttgcgggcg gctcgtttgc
     7021 catcgctcag taccttagct ccaacttcat tgggccggag ctgccggaca ttatctcttc
     7081 gctggtatca ctgctctgcc tgacgctgtt cctcaaacgc tggcaaccag ttcgcgtatt
     7141 ccgcttcggt gacttggggg cgtcacaggt tgatatgacg ctggcccaca ccggttacac
     7201 cgcgggtcag gtgttacgtg cctggacacc gttcctgttc ctgaccgcta ccgtaacact
     7261 gtggagcatc ccgccgttta aagccctgtt cgcatcgggt ggtgcgctgt atgagtgggt
     7321 gatcaacatt ccggtgccgt acctcgataa actggttgcc cgtatgccac cagtggtcag
     7381 cgaggctaca gcctatgccg ccgtgtttaa gtttgactgg ttctctgcca ccggcaccgc
     7441 cattctgttt gctgcgctgc tctcggttgt ctggctgaag atgaaaccgt ctgactctat
     7501 cagcaccttc ggcagcacgc tgaaagaact ggctctgccc atctactcca tcggtatggt
     7561 gctggcattc gcctttattt cgaactattc cggactgtca tcaacactgg cgctggcact
     7621 ggcgcacacc ggtcatgcat tcaccttctt ctcgccgttc ctcggctggc tgggggtatt
     7681 cctgactggg tcggatacct catctaacgc cctgttcgcc gcgctgcaag ccaccgcagc
     7741 acaacaaatt ggcgtctctg atctgttgct ggttgccgcc aataccaccg gtggcgtcac
     7801 cggtaagatg atctccccgc aatctatcgc tatcgcctgt gcggcggtag gcctggtggg
     7861 caaagagtct gatttgttcc gctttactgt caaacacagc ctgatcttca cctgtatggt
     7921 gggcgtgatc accacgcttc aggcttatgt cttaacgtgg atgattcctt aatgattgtt
     7981 ttacccagac gcctgtcaga cgaggttgcc gatcgtgtgc gggcgctgat tgatgaaaaa
     8041 aacctggaag cgggcatgaa gttgcccgct gagcgccaac tggcgatgca actcggcgta
     8101 tcacgtaatt cactgcgcga ggcgctggca aaactggtga gcgaaggcgt gctgctcagt
     8161 cgacgcggcg gcgggacgtt tattcgctgg cgtcatgaca catggtcgga gcaaaacatc
     8221 gtccagccgc taaaaacgct gatggccgat gatccggatt acagtttcga tattctggaa
     8281 gcccgctacg ccattgaagc cagcaccgca tggcatgcgg caatgcgcgc cacacctggc
     8341 gacaaagaaa agattcagct ttgctttgaa gcaacgctaa gtgaagaccc ggatatcgcc
     8401 tcacaagcgg acgttcgttt tcatctggcg attgccgaag cctcacataa catcgtgctg
     8461 ctgcaaacca tgcgcggttt cttcgatgtc ctgcaatcct cagtgaagca tagccgtcag
     8521 cggatgtatc tggtgccacc ggttttttca caactgaccg aacaacatca ggctgtcatt
     8581 gacgccattt ttgctggtga tgccgacggg gcgcgtaaag caatgatggc gcaccttagt
     8641 tttgttcaca ccaccatgaa acgattcgat gaagatcagg ctcgccacgc acggattacc
     8701 cgcctgcccg gtgagcataa tgagcattcg agggagaaaa acgcatgatt atttccgcag
     8761 ccagcgatta tcgcgccgca gcgcaacgca ttctgccgcc gttcctgttc cactatatgg
     8821 atgggggtgc atattctgaa tacacgctgc gccgcaacgt ggaagatttg tcagaagtgg
     8881 cgctgcgcca gcgtattctg aaaaacatgt ccgacttaag cctggaaacg acgctgttta
     8941 atgagaaatt gtcgatgccg gtggcgctag gtccggtagg tttgtgtggc atgtatgcgc
     9001 gacgcggcga agttcaggca gccaaagcgg cggatgcgca tggcattccg tttactctct
     9061 cgacggtttc cgtttgcccg attgaagaag tcgcgccagc catcaagcgc ccaatgtggt
     9121 tccagcttta tgtactgcgc gatcgcggct ttatgcgtaa cgcgctggag cgagcaaaag
     9181 cagcgggttg ttcgacgctg gttttcaccg tggatatgcc gacaccggga gcgcgttatc
     9241 gtgatgcgca ttctggaatg agcggcccga acgcggcaat gcgccgctat ttgcaggcgg
     9301 tgacgcatcc gcaatgggcg tgggatgtgg gcctgaacgg tcgtccgcat gatttaggaa
     9361 atatttcggc ttacctcggc aaaccgaccg gactggaaga ttacatcggc tggctgggga
     9421 ataacttcga tccgtccatc tcatggaaag accttgagtg gatccgcgat ttctgggatg
     9481 gcccgatggt gatcaaaggg atcctcgatc cggaagatgc gcgcgatgca gtacgttttg
     9541 gtgctgatgg gattgtggtt tctaaccacg gtggccgcca gctggacggt gtgctctctt
     9601 ctgcccgtgc actgcctgct attgccgatg cggtgaaagg tgatatcgcc attctggcgg
     9661 atagcggaat tcgtaacggg cttgatgtcg tgcgtatgat tgcgctcggt gccgacaccg
     9721 tactgctggg tcgtgctttc ctgtatgcgc tggcaacagc gggccaggcg ggtgtagcta
     9781 acctgctaaa tctgatcgag aaagagatga aagtggcaat gacgctgact ggcgcgaaat
     9841 cgatcagtga aattacgcaa gattctctgg tgcaggggct gggtaaagag ttgcctacgg
     9901 cactggctcc gatggcgaaa gggaatgcgg catagtcgtt tgagccccct caccctaacc
     9961 ctctccctca gggagtgggg accgttcggc gctgtatgta ctccctcact ctgaaacgac
    10021 accgcactct ttttttctcc ctcgcccctc cggggtgagg ggaaaaggcc gcactgctca
    10081 ccaattttct gctatcctgc ccccgcacta agggggcagt atgctaaaca tcgtacttta
    10141 cgaaccagaa attccgccaa atactggcaa tatcatccgt ctttgcgcta ataccggctt
    10201 tcgtctgcat atcatcgaac cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc
    10261 ggggctggac tatcacgagt ttaccgccgt tacgcgtcat catgactttc gcgcgttcct
    10321 cgaagcagaa aatccccagc gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag
    10381 cgccgtaaac tatcaggatg gcgattatct gatgttcggc ccggaaacac gcggcctgcc
    10441 agcgagcatt cttgatgccc tgcccgctga acaaaaaatt cgcattccga tggtgccgga
    10501 cagccgcagc atgaacctgt ccaatgcggt gtcagttgtg gtgtatgaag cctggcggca
    10561 gctgggatat ccgggagcgt tgttgagaga ttagttactg tatgccggat agatgattac
    10621 atcgcatccg gcacgatcac aggacattag atcccatccc catactcaaa tgtatggtta
    10681 ataccgttga aatgctggtc catatccatt gatggcttat cgctgtctgg tttaccgaca
    10741 atacgagccg gaacgccagc ggcggttgta tgcggcggca ccggttgcag caccacggaa
    10801 cctgcgccaa tcttcgcgcc gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg
    10861 ccaatcatca caccttcacg aattttcggg tgacggtcac caccagattt acccgtaccg
    10921 ccaagcgtca cagattgcag aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca
    10981 acgatgcccg tggcgtggtc gagcataatg ccacggccaa tttttgctgc cgggtgaata
    11041 tcgacctgga acgtcacaga aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc
    11101 tgattccaca accagtgacc gatgcgatag gcctgcaagg catgaaaacc cttcaggtat
    11161 aacaacgggg ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca
    11221 caggccgcag aggcgatcat ttccgggtca gcggcgtagg cttcttccac cacttcacgg
    11281 atagcaatag caggcataat tggcgatgac agcttgttcg ccagcatgta gctcagtgca
    11341 cttccaaggt tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag cattggctca
    11401 cagtccgcca gcgttctggc ttcggcttta atattgttcc agacaatttc cagttcttca
    11461 cacgacattg cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt
    11521 catagcggta acaaaggttc cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg
    11581 acctaataac gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat
    11641 ttcctcggtt attggcattt ctacgccgaa gcgatgcgcc agttcgcgga cttctttcgt
    11701 attgcggtag ccttccacca cctgaccaat cttctcctgc gcgctttgta catccatgcc
    11761 ctgaccgagc atcatgccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac
    11821 cagatccccc agccccgcca tgcccataaa ggtggcagga tcggcaccca gcgccgcgcc
    11881 aagacgcgac atttcagcca gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa
    11941 accgataccg tcggacatcc ccgcaccaat ggcaatgacg tttttcaccg cgccgccaag
    12001 ctgcacgcca atgaaatccg ggttactgta aacgcggaaa cttttgccgc agtgcagcag
    12061 ctgctgaaga tcatcagcaa aggtctgatc ggtcgaggcc agcgaaatag ctgtcggtaa
    12121 acctgccgcc agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc
    12181 gcctaacgcc tcgcgggcca cgtcctgtaa cagacgcccg gtttccgctt ccagcccttt
    12241 ggtcgcccac accagacgcg catcaggacg catcagcggt ttaatctggc gcagcacttc
    12301 accaaagaca tggctgggta cgacgacgag aatattacgg ctggctgcca gcgcagtggc
    12361 gagatcgctt tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt
    12421 acagcggtcg cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc
    12481 gtggccattt cttgccaggg taatggcaag agcggtgccg tacgagccgg caccgatcac
    12541 agtcattgaa gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca
    12601 gcctgctgct gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt
    12661 tgcgggaatg taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga
    12721 atgttcgggc agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg
    12781 gagaaaatac cgccctgctg aacttcacac aggaacgcgg tttcttcgcc cagggaggcc
    12841 gttacggtaa cacgcagtac cacttcgtaa acgtcatctg ccagttgagt agatgccgta
    12901 tccagatcaa gtttaacttc tggctgccaa tctttctgga aaacgtgcgg cgcgttcggc
    12961 gcttcgaaag aaatatcctt ggtataaata cgttggatct ggaaagtcat ttcggtgttg
    13021 ttttgttctg acatgtgtag aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa
    13081 tgccaacgtt cacacgttat ttcagcaggg gatccagtcc accacgtgca tccaatgcat
    13141 acaagtcatc acagccgcca atgtgctgtg cgccaataaa aatctgggga accgtggtgc
    13201 gaccgctgcg tttgatcatc tcttcacgct tggcggcatt gccatcgatc ggcaactcct
    13261 gaaaactcac gcccttgctg ctcagcagtg cttttgcacg atggcaatac gggcaggttt
    13321 ctttggtata gatttcaaca ttggccataa tttagctcct gtttttttac cctgaaattt
    13381 catgttgcag ggcggctgca aaatggagtg tccccagaag cttataacaa taagtcgctg
    13441 ggggcctaat tgcagctaac ggcctgcatc atgaaagacg acaggtaaat tatttgccgc
    13501 gcaccaaagg caagttttcg ccagcccagc cagcgacgcc ttctttcagc acgaatactt
    13561 gcgcaaaacc agctttcgtc agtgcgtttg caggctcctg gcactgcatg ccagaaccgt
    13621 ctaccacgat aaccggtttg tctttgtgct tctcaagctc accaacattg ttgactttga
    13681 tttcgctcgg caacagattg atagaacctg cgatatggcc tttacggaag tcatcacgct
    13741 gacgtaaatc cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgag
    13801 taatcacctt cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta
    13861 acgcgatcca ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca
    13921 tggggggtaa caactcccga cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc
    13981 ggtgcggcaa atacagccag cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa
    14041 aaaagcgcaa atctggcatc ggcttgccgc gagcggtcgt atattttgat cttcagaggc
    14101 tattttatcg attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg
    14161 tcgcaatgtc ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc
    14221 gtgaagaaca gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt
    14281 gggccaatcg tccgcatacc ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc
    14341 gtcagatggg taactccgaa gtaggccacg ttaacctggg tgccggccgc atcgtgtatc
    14401 aggacctgac tcgtctggac gttgaaatca aagatcgcgc tttctttgct aatccagtgc
    14461 tgactggcgc ggtagataaa gcgaaaaacg caggtaaagc agtacacatt atgggtctgc
    14521 tctccgcagg cggcgtacac agccacgaag atcacatcat ggcgatggta gaactggcag
    14581 ctgaacgcgg cgcagaaaaa atctacctgc acgcattcct tgacggtcgc gacaccccgc
    14641 cgcgcagtgc tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag
    14701 gccgcgtagc gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc
    14761 gcgttgaaaa agcctatgac ctgctgactc tggcgcaggg tgagttccag gccgatactg
    14821 ccgttgctgg tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga
    14881 ccgttattcg tgcagaaggt cagccagatg cggcaatgga agacggcgat gcgctgattt
    14941 tcatgaactt ccgtgctgac cgcgcgcgcg aaatcactcg tgcattcgta aacgcagatt
    15001 tcgacggctt cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg
    15061 ccgctgacat caaaactgcg gttgcttacc cacccgcttc cctggttaac accttcggcg
    15121 agtggatggc gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc
    15181 acgttacttt cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc
    15241 tgatcaactc accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc
    15301 tgaccgaaaa actggttgcg gccatcaaga gcggcaaata cgacaccatt atctgtaact
    15361 atccgaacgg cgacatggta ggtcacaccg gggtgatgga agcggcggtt aaagcggttg
    15421 aagcactgga tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac
    15481 tgctgatcac cgctgaccac ggtaacgctg aacagatgcg cgatccggca acgggtcagg
    15541 cacacacggc acacaccaac ctgccagttc cgctgattta cgttggtgat aagaacgtga
    15601 aagcggttgc aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta
    15661 tggaaatccc gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca
    15721 tgaggggaaa ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca
    15781 ggcccatcat ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc
    15841 acgcggatga gcgtgaccaa ctcaaatcta ttcaggccga tatcgccgcg aaagagcgcg
    15901 cggtacgcca aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag
    15961 aagcgatctc tgaagccacc cgtaagctgc gcgaaacgca aaacacgctc aatcaactca
    16021 ataaacagat tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc
    16081 aggagcgcag cctcgccgca caactggatg ccgcattccg tcagggcgag cataccggta
    16141 ttcagctaat tctcagcggt gaagaaagcc agcgtggaca gcgtttacag gcttatttcg
    16201 gctatctcaa ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag
    16261 tcgccatgca gcgtgctgaa ctggaagaga aacaaagcga gcaacaaacg ctgttatatg
    16321 aacagcgcgc ccaacaggcg aaactgactc aggctctgaa cgagcgtaaa aagacgctgg
    16381 cggggctgga atcttccatc cagcaaggtc agcagcagct aagtgaactg cgtgccaacg
    16441 aatcccgtct gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac
    16501 gagaagcgcg tgaggcccag gcggttcgcg accgccagaa agaagcgacg cgcaaaggca
    16561 ccacctataa accgaccgaa agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg
    16621 cgccgcgtgg tcaggcattc tggccggttc gcgggccgac gctgcatcgc tatggtgaac
    16681 agctacaggg cgaactacgc tggaaaggaa tggttatcgg tgcctctgaa ggtactgaag
    16741 ttaaagcgat tgccgacggt cgggtgattc tggctgactg gctgcaaggt tacggtctgg
    16801 tggtggtggt tgagcacggt aaaggcgaca tgagtcttta cggctataat cagagcgcac
    16861 tggtgagcgt tggttcgcag gttcgcgcgg gccagccaat tgcactggtg ggcagcagtg
    16921 gcggtcaggg tcggccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc
    16981 cacagccgtg gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg
    17041 ccgctctgtt ggcgctctcc tcccccgtac ttgctggcaa acttgccatc gtcattgatg
    17101 attttgggta tcgcccgcac aacgaaaacc aggtgctggc gatgccctcc gctatctccg
    17161 tcgctgtatt acccgattca ccgcacgcca gagagatggc gaccaaagcg cataacagcg
    17221 ggcacgaagt gttgattcat ctcccgatgg caccactgag taaacagccg ctggagaaaa
    17281 atacgctgcg cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca
    17341 ataacgtgcc ctatgccgtg gggatcaaca accacatggg tagcaagatg acctctaacc
    17401 tgtttggtat gcagaaagtg atgcaggcac tggagcgtta caatctttac ttcctcgaca
    17461 gcgtaaccat cggtaatacc caggcgatgc gcgccgcgca aggtactggc gtgaaggtga
    17521 tcaaacggaa ggtgttcctc gacgattcgc agaacgaagc ggacatccgt gtgcaattta
    17581 atcgcgcaat tgatctggcg cgtcgcaacg gttcaaccat tgccattggg catcctcacc
    17641 cttcaacggt acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg
    17701 tgaaagccag cagcttgctg aatgaaccgc aggttgatac ttctacaccg ccgaaaaacg
    17761 ctgtgcctga tgcaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac
    17821 cgatagaacc tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga
    17881 gcacgctgat cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cccgaagcgg
    17941 cgaagtttaa cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt
    18001 tttgtcgcgc cattgccata agcggaaaga ccacagtaac gcctggtaac cgaccttcac
    18061 gctgcgcaca ttggtaatca ggcgcttata cataccggaa gtaaatatct cggcaatcat
    18121 ccgttggcgg gtaagaatat ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc
    18181 gtaagttatt tgctgatgaa attccggata aatcataatt ttgtcggcat aatttcgatt
    18241 taatttctcc agcaggcggg taatcttaat atagtgacgt tgataattaa ggtttttatt
    18301 cccttgtcta tgcaaccgac tcactgacgt attatgcaga taatatttat ataatgattg
    18361 ctcggtatat cgcgcacgca gcgcgttaaa catgaattct gttgtccaga caatatcctg
    18421 atgatgtaat ccggcaataa atttaatgtt atttttaaca ataacatcac ggcgataaac
    18481 ccccatccag acaacgtgag tccaacggcg cgaagaaagc cccatccgca gccagtccgg
    18541 gccggttaat acaccggttg agcgaaggcg atcgctgggg atggattgcc aggtttctcc
    18601 cgtttcacga aaagaccagt cagcgttgca ctgcgccacg tcgaggtcgt cctctaacgc
    18661 catggtcatc agcgtttcgt acatggtggg atagacctca tcgtcagcat cgacaaaagc
    18721 gacatatttg cccgtcgcca cctcgatccc acgattacgc gctaccgatg cgccagcatt
    18781 cgcctgatgc aacaaacgaa cgtgcggata gttttctgcg taatgctttg ctatttcaac
    18841 agaattatcc gttgaaccat cgttaataat aatgatttcc agagcagtcc aggtttgcgt
    18901 aattaaagat tccatacaag tgcggaaatc atcgcccgca ttatataacg gaataataac
    18961 actaagttta ttggtgctgt tcatcataaa ttaccgacga cctctgaagt tatcctgacg
    19021 ttttacaggc tttgtattaa gaaactatta agcctgtgag atattatttt tacaggtgag
    19081 agagtcactt cagggtagag agataatgag aacagctata taaaagatca gcgcaattac
    19141 gaactcttta ttacactgac aggattttac atatttaaca gattatcctc aggcaaaagg
    19201 gctggaatac cagccctttt tcgtgttaat cccagctcag aataactttc ccggactggc
    19261 ccgaacacat agcgtcaaag cccttctgga aatcatcgat agagaaacga tgggtaatga
    19321 tcggcgagag atcgaggcca gactgaatca gcgccgccat cttgtaccag gtttcaaaca
    19381 tctcacgacc gtaaatgcct ttaatgaaca agcctttaaa gatcactttg gtccagtcga
    19441 tagacatgtc agacggtgga atacccagca tcgcaatacg accgccgtgg ttcatggtat
    19501 cgagcatggt acgaaacgct ggcggcgcgc cggacatttc cagaccgaca tcaaagcctt
    19561 cggtcatgcc cagttcagcc atcacatcat taaggttttc tttcgcgacg ttaaccgcac
    19621 gggtgatacc cattttgcgc gccagctcaa ggcggtattc gttaacatca gtgatcacca
    19681 cattgcgtgc accaatgtgt ttcgccaccg ccgcagccat aatacctatc ggacctgcac
    19741 cagaaaccag cacatcttcg cccaccagat cgaacgacag cgcggtatgc acggcgttac
    19801 cgaagggatc aaaaatggaa gccaggtcgt cggagatatt gtcggggatt ttgaaagcgt
    19861 tgaacgccgg aatcaccaga tattcggcga agcagcccgg acggttaaca ccgacaccaa
    19921 tggtgttgcg gcacagatgg gtacgaccgc cacgacagtt gcgacagtga ccgcaggtga
    19981 tatggccttc gccagaaacg cgatcgccga ttttgaagcc tttcacttcc tgaccaatac
    20041 ctaccacttc accgacatat tcatggccta cgaccatcgg caccgggatg gttttttgcg
    20101 accactcatc ccagttatag atgtgaacgt cagtcccgca gatggctgtt ttacggattt
    20161 taatcagcag atcgttatgc ccgagttccg gtacaggaac gtcggtcatc cagatgccct
    20221 cttccgcttt cagtttggat aacgctttca tctcacatcc tcaggcgata acgcccagtt
    20281 gtttaccaat acgcgtgaat gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg
    20341 ccgacatctg ggtacgaata cgcgcctgac ctttcggaac gaccggatag aagaaaccgg
    20401 tgacgtaaat gccctctttt tgcagctcac gggcaaattt ctgcgccact accgcatcac
    20461 caagcatgac cggaataatg gcgtgatcgg ctcccgccag ggtaaagccc gccgccgaca
    20521 tttgctcacg gaactgacgc gcgttcgccc acagacggtc acgcaactcg ctacccgctt
    20581 caaccatctc cagcactttg atggacgcgg caacaatggc cggtgccagc gagttggaga
    20641 acaggtacgg acgagaacgc tggcgcagcc actcaaccac ttctttgcgc gccgcggtat
    20701 aaccaccaga agccccgccc agcgctttac cgagcgtgcc ggtgatgatg tcgacacgcc
    20761 ccatcacatc gcagtattca tgggaaccac gaccattttc accgacaaaa ccgaccgcgt
    20821 gggagtcgtc taccatcacc agggcatcgt atttatctgc cagatcgcaa acgcccttca
    20881 gattggcaat cacaccgtcc atcgagaata caccatcggt ggcaatcagc acatgacgcg
    20941 caccggcttc acgcgcttct ttcagacgag cttcaagctc ctgcatatcg ttgttggcat
    21001 agcgatagcg tttagcttta cacagacgca caccatcaat aatagacgcg tggttcagtg
    21061 cgtcggagat aatggcgtct tccgcgccca gcagcgtttc aaacaggcca ccgttagcat
    21121 caaagcagga agagtagaga atcgcatctt ccatccccag gaatgccgcc agtttttgtt
    21181 caagctcttt atggctgtcc tgagtgccgc aaataaaacg caccgaagcc atgccgaaac
    21241 cgtgagaatc cattcccgcc tttgctgccg caatcagatc cggatgattc gccagcccga
    21301 gatagttgtt ggcacaaaag ttaatgacgt ggcttccatc agccacggtg atatctgctt
    21361 gctgcgcaga cgtaataatg cgctcttctt taaacaaccc ttccgcccgt gcggtttcca
    21421 gatcgttggt taactgctga taaaattctc cacgcattgc gattctccag acagggcaaa
    21481 ttccagcaca tattacccaa acttataggt cgcgacgaga tgacgcgtta acacttctgc
    21541 aaaattcagg ataaataacg tccatcaatc tcacttttgg cgaatggctg aagccaacgc
    21601 cgtgtagtga tatgatagaa gtattcgtgt ctgagattgt ctctgactcc ataattcgaa
    21661 ggttacagtt atgatcatcg ttaccggcgg cgcgggcttt atcggcagca acatcgttaa
    21721 ggccctgaat gataaaagca tcaccgatat tctggtggtg gacaacctga aagacggcac
    21781 caagtttgtg aacctggtgg atctggatat cgcggactat atggataagg aagacttcct
    21841 gatccagatt atggctggcg aagagttcgg cgatgtcgaa gcgattttcc acgaaggcgc
    21901 atgctcttcc accaccgagt gggacggcaa gtatatgatg gataacaact atcaatactc
    21961 caaggagctg ctgcactact gcctggagcg cgaaatcccg ttcctgtacg cttcttccgc
    22021 agccacctac ggcggacgca cctccgactt tattgaatcc cgcgagtacg aaaaaccgtt
    22081 gaacgtctac ggttactcaa aattcctgtt tgatgaatat gttcgccaaa tcctgcctga
    22141 agcgaactcg cagattgttg gcttccgcta tttcaacgtt tatggaccgc gtgaaggcca
    22201 taaaggcagc atggcgagcg tcgctttcca tctcaacact cagcttaaca aaggtgaatc
    22261 accgaagctg tttgaaggta gcgagaactt caaacgtgac ttcgtctatg tgggcgacgt
    22321 ggcagatgta aacctgtggt tcctggaaaa tggcgtttcc ggtatcttca acctcggtac
    22381 tggtcgtgcg gaatccttcc aggccgtagc agatgctact ctggcttatc acaagaaagg
    22441 ccagatcgaa tacattccgt tcccggataa gctgaaaggc cgctaccagg cgttcactca
    22501 ggcagatctg acaaatctgc gcgcggcggg ttacgacaaa ccgttcaaaa ccgttgctga
    22561 aggtgtaatg gaatacatgg cctggctgaa tcgcgacgca taagagctct gcatgaaaat
    22621 actggtgatc ggcccgtctt gggttggcga catgatgatg tcgcaaagtc tctatcgcac
    22681 gctccaggcg cgctatcccc aggcgataat cgatgtgatg gcaccggcat ggtgccgtcc
    22741 attattatcg cggatgccgg aagttaacga agctatccct atgcctctcg gtcacggagc
    22801 gctggaaatc ggcgaacgcc gcaaactggg tcatagcctg cgtgaaaagc gctacgaccg
    22861 cgcctacgtc ttaccaaact ccttcaaatc tgcattagtg cctttcttcg cgggtattcc
    22921 tcatcgcact ggctggcgcg gcgagatgcg ctacggttta ctcaacgatg tacgcgtgct
    22981 cgataaagaa gcctggccgc taatggtgga acgctatgtc gcgctggcct atgacaaagg
    23041 cattatgcgt accgcacaag atctgccgca gccattgtta tggccgcagt tgcaggtgag
    23101 cgaaggtgaa aaatcatata cctgtaatca attttcgctt tcatcagaac gtccgatgat
    23161 tggcttttgc ccgggtgcgg agtttggtcc ggcaaaacgc tggccacact accactatgc
    23221 ggagctggca aagcagctga ttgatgaagg ttatcaggtg gttctgtttg gctctgcgaa
    23281 agatcatgaa gcgggcaatg agattcttgc cgctttgaat accgagcagc aggcatggtg
    23341 tcggaacctg gcgggggaaa cacagcttga tcaagcggtt atcctgattg cagcctgtaa
    23401 agccattgtc actaacgatt ctggcctaat gcacgttgcg gcggcgctca atcgtccgct
    23461 ggttgccctg tatggtccga gtagcccgga cttcacaccg ccgctatccc ataaagcgcg
    23521 cgtgatccgt ctgattaccg gctatcacaa agtgcgtaaa ggtgacgctg cggagggtta
    23581 tcaccagagc ttgatcgaca ttactcccca gcgcgtactg gaagaactca acgcgctatt
    23641 gttacaagag gaagcctgac ggatgcgggt tttgatcgtt aaaacatcgt cgatgggcga
    23701 tgttctccat acgttgcccg cactcactga tgcccagcag gcaatcccag ggattaagtt
    23761 tgactgggtg gtggaagaag ggttcgcaca gattccttcc tggcacgctg ccgttgagcg
    23821 agttattcct gtggcaatac gtcgctggcg taaagcctgg ttctcggccc ccataaaagc
    23881 tgaacgcaaa gcgtttcgtg aagcgctaca agcagagaac tatgacgcag ttatcgatgc
    23941 tcaggggctg gtaaaaagcg cggcgctggt gacgcgtctg gcgcatggcg taaagcatgg
    24001 attggactgg caaaccgctc gcgaaccgtt agccagcctg ttttacaatc gtaagcatca
    24061 tattgcaaaa cagcagcacg ccgtagaacg cacccgcgaa ctgtttgcca aaagtctggg
    24121 ctatagcaaa ccgcaaaccc agggcgatta tgctattgca cagcatttcc tgacgaacct
    24181 ccctacagat gctggcgaat atgccgtatt tcttcatgcg acgacccgtg atgataaaca
    24241 ctggccggaa gaacactggc gagaattgat tggtttactg gctgattcag gaatacggat
    24301 taaacttccg tggggcgcgc cgcatgagga agaacgggcg aaacgactgg cggaaggatt
    24361 tgcttatgtt gaagtattgc cgaagatgag tctggaaggc gttgcccgcg tgctggccgg
    24421 ggctaaattt gtagtgtcag tggatacggg gttaagccat ttaacagcgg cactggatag
    24481 acccaatatc acggtttatg gacctaccga tccgggatta attggtgggt atgggaagaa
    24541 tcagatggtt tgtagggctc cggggaatga gttgtctcaa ttgacagcaa atgctgttaa
    24601 gcggttcatt gaagaaaacg ctgccatgat ttaaattaca actctttaat agataatttt
    24661 gaaataaaat cagcttcctt gctgatttta tttcacatat tcattacttc tctaataaac
    24721 attggtccga ttgtgcttta aaataagcac aaagcaatat tatggtgata aacacagcag
    24781 tcggaaccgg tttagaaaag agtattacat cacttagtcc tactaccagt agtgaaaaaa
    24841 gcaagattaa aatataaggt tctttagcag ttcttaaggc atatatgagt attgctgaaa
    24901 atgctaaaat tgttaataca actccaggaa taccacgcgt tgataacgta tctatgagat
    24961 cgttatgcaa atgagaatct acatagggta aagcgccact caatctaggc tctttttctt
    25021 ctagttcatg tattttttcc gcacgttttt ccagtgattg tcctattgga gaatatgttt
    25081 ttaagccaac ttcatacatt gccagacggg caccgactga agttctggtg ttatcatgcg
    25141 aataattaac taaatcgtta tttaagtcat tcattctcat aagcaatgta tctttaaaag
    25201 aaaatacacc tgctaataat gcaatcgtaa taaaacaata taacttataa ttaattcgct
    25261 tattaatata actatccatt acgattaata ttaacgcaaa gaatggaaac actaatatgg
    25321 ctgcacgcgt ctcagttact atgacagcac ataaactaag catgaagtta attgtatata
    25381 atgttgtttt atgtcttaag tcggatttta aaatgagaat tgatgccagt aaagaaggaa
    25441 aaaggatgat gtagcctgct gttgtagcgt gggaagctga taatgcaaca cggtcgacgt
    25501 ttaaatagaa atgttgccac atggcatgcg caaaaaaaag taactgcgtt gccagggatg
    25561 cgaacagaat tagtttccca aattttattt ttgttctcat ctcattttta gcaaaaagaa
    25621 ctaagaaaat aaaagcgcta cacaaggcaa tttttccagt tt
//
LOCUS       DAOFQD010000067        24037 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.067, whole genome shotgun sequence.
ACCESSION   DAOFQD010000067 DAOFQD010000000
VERSION     DAOFQD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.067"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(1..648)
                     /gene="gloC"
                     /locus_tag="QBF46_002781"
     CDS             complement(1..648)
                     /gene="gloC"
                     /locus_tag="QBF46_002781"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDQ6674942.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(675..1223)
                     /locus_tag="QBF46_002782"
     CDS             complement(675..1223)
                     /locus_tag="QBF46_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDQ6674943.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1404..3251)
                     /gene="ldtD"
                     /locus_tag="QBF46_002783"
     CDS             complement(1404..3251)
                     /gene="ldtD"
                     /locus_tag="QBF46_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDQ6674944.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3512..7972)
                     /gene="mukB"
                     /locus_tag="QBF46_002784"
     CDS             complement(3512..7972)
                     /gene="mukB"
                     /locus_tag="QBF46_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDQ6674945.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIAELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(7972..8676)
                     /gene="mukE"
                     /locus_tag="QBF46_002785"
     CDS             complement(7972..8676)
                     /gene="mukE"
                     /locus_tag="QBF46_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDQ6674946.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8657..9979)
                     /gene="mukF"
                     /locus_tag="QBF46_002786"
     CDS             complement(8657..9979)
                     /gene="mukF"
                     /locus_tag="QBF46_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDQ6674947.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(9976..10761)
                     /gene="cmoM"
                     /locus_tag="QBF46_002787"
     CDS             complement(9976..10761)
                     /gene="cmoM"
                     /locus_tag="QBF46_002787"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDQ6674948.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            10897..11676
                     /gene="elyC"
                     /locus_tag="QBF46_002788"
     CDS             10897..11676
                     /gene="elyC"
                     /locus_tag="QBF46_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDQ6674949.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGLAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11653..12546)
                     /locus_tag="QBF46_002789"
     CDS             complement(11653..12546)
                     /locus_tag="QBF46_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDQ6674950.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12700..13446)
                     /gene="kdsB"
                     /locus_tag="QBF46_002790"
     CDS             complement(12700..13446)
                     /gene="kdsB"
                     /locus_tag="QBF46_002790"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDQ6674951.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIRNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13443..13625)
                     /gene="ycaR"
                     /locus_tag="QBF46_002791"
     CDS             complement(13443..13625)
                     /gene="ycaR"
                     /locus_tag="QBF46_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDQ6674952.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13677..14909)
                     /locus_tag="QBF46_002792"
     CDS             complement(13677..14909)
                     /locus_tag="QBF46_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDQ6674953.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(14946..15932)
                     /gene="lpxK"
                     /locus_tag="QBF46_002793"
     CDS             complement(14946..15932)
                     /gene="lpxK"
                     /locus_tag="QBF46_002793"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDQ6674954.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(15929..17677)
                     /gene="msbA"
                     /locus_tag="QBF46_002794"
     CDS             complement(15929..17677)
                     /gene="msbA"
                     /locus_tag="QBF46_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDQ6674955.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17714..19978)
                     /locus_tag="QBF46_002795"
     CDS             complement(17714..19978)
                     /locus_tag="QBF46_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDQ6674956.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPAFRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAIVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20185..20469)
                     /gene="ihfB"
                     /locus_tag="QBF46_002796"
     CDS             complement(20185..20469)
                     /gene="ihfB"
                     /locus_tag="QBF46_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDQ6674957.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20629..22302)
                     /gene="rpsA"
                     /locus_tag="QBF46_002797"
     CDS             complement(20629..22302)
                     /gene="rpsA"
                     /locus_tag="QBF46_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDQ6674958.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22413..23096)
                     /gene="cmk"
                     /locus_tag="QBF46_002798"
     CDS             complement(22413..23096)
                     /gene="cmk"
                     /locus_tag="QBF46_002798"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDQ6674959.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23269..24033)
                     /gene="ycaL"
                     /locus_tag="QBF46_002799"
     CDS             complement(23269..24033)
                     /gene="ycaL"
                     /locus_tag="QBF46_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDQ6674960.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         5740 a         6622 c         5893 g         5782 t
ORIGIN      
        1 ttaccagacg ggcatttcgt cttgcaggaa gggattatgc aggcgttcat aaccaagtgt
       61 ggataatggt ccgtgacccg gaataaatgt cacgtcatcc cccagtggca gcaatttatc
      121 tttgattgaa gaaatcagtt gattatgatc gccacgcggg aagtcactgc gccctactcc
      181 gcctttgaaa ataacatcgc cagaaatcag cagctttgcc cgatcatcaa aaaacacgac
      241 atgacccggc gtatgcccag ggcaatgtaa cacctgtaaa gtcacattcc ctatgctgat
      301 ggtatcgcct tcgttcagcc aacgatctgg cgtcagcggc tggcactctt ccagaccaaa
      361 catacgactt tgcgcaggca agccttgcag ccagaactca tcttcttttt ccgggccgaa
      421 aaccggcacg ccgtaatgtt gcgccagttc cgccgctgcg ccaacgtggt ccagatgacc
      481 atgcgtcagc aggatctgca tcagtgtcag gccgctgtca tcaacttcct gtttgatttt
      541 ttccgcatcg ccgccaggat cgaccagtgc ggccagacgg gtttgttcac accagattaa
      601 tgaacagttc tgggagaatg cggtgaccgg aataatacga tagttcatac tgcccctgtt
      661 tcgttaagca attactacca gtgccgtgct ggcccggtat caatatgcac aaagttacta
      721 cgtggataat atcctacacc acctgcgcgc atagataacg cggctttgcg aatattgctt
      781 aacgcgatac cttcaatatg gaaatccatc gcctggcctt tagtgtgata gcttttcttc
      841 gctactccac ggctgcgggc gcgtagttca ttgttggtat caatagaacg ataaccggaa
      901 atgagttgca ccggtttgcg cgtgcctaac aacccttgca ggcgatacaa ctggtcgaat
      961 aatcctgggt cgatggactt tattttgttc gcgcggtaat cgcggaaaaa atggttaagt
     1021 tttgccaatt cttcctgaat atagcctctg ccatcgaaaa actccgcttt gattgactct
     1081 ccggtatgaa gattattgag tgtcaaaatg cgcgggcgtg gggtagagag tgttgcaaac
     1141 gcaggggtcg gcaggatggc ggcaccgagt gcaacgccac caagcgccag caatttgcgg
     1201 cgattagcgt cgaatttgtc catgataatc aagtctacag gtcaatgtta tcgtttatat
     1261 gcacttctgg cgcacaagag gcaccttaac cgcccgtaaa gcagacgtca agatgactca
     1321 accccagcag tgacgggggc tgcagagaat cgcccccaat catgctaatt attacgacaa
     1381 ctgatttccc cgaactactt catttacctg attaattgtt ccgctttcga tacgatttgc
     1441 gagctggatc gcgcaggcag atcataattg taaatatctg tacgatactg ggtacgacca
     1501 tctgcaccaa caaaggccgt caggtagtag agattcaccg gaatcgactg ccgaatattg
     1561 acgtaacgtg tatcaccctg cttcagcgca tcagaaatac gtttgtcatt ccagcctgca
     1621 tcctgcaaca gcatattcgc cagatcggaa gctttattca ctcgtacaca gcctgagctc
     1681 aatgcgcgtg tatcacgctt gaacagattg tggttcggcg tgtcatgcaa ataaatggcc
     1741 tctgaactcg gcatattgaa tttatagcgc cccagcgagt tccgtgggcc tggagcctgc
     1801 tggaagcgga acggtaaatt cgaggccgtg attgtagacc agtcaacctg ccatgggtca
     1861 atcgcttctc tgctgttcca gccgcgcatc accgtatagc catggctttc gagatatccc
     1921 ggatcgttgc gcacttttgg cagaatatct ttgcgtgcca gagttggagg tacgttccac
     1981 ggcgggttta ccactacgtt gttaagggca ctgctcatca tcggcgtttt gcgatcgggg
     2041 cgaccgacaa tgactcgcga atccagcacc tgattgccgt tctgatagta gaccagcgaa
     2101 taggccggaa tgttaaccat gatcccggta gaaagctctg ttggcagcaa gcgcaatcgc
     2161 tggatgttga gagccaacac gccagcacgc tgggcgggcg ttacgtttaa ccagtcacgt
     2221 gttgccgggc caatagcacc atctgccccc aatccttgcc atgcctgaaa acgtttaacg
     2281 gcttccacca gttcattatc gtaggcggca cgaacggcag gcgcaggttt actacgagac
     2341 gtcgtttgct tgtccatcgg cttagtttct gctgtttcaa cagtaacagc ggatgggctg
     2401 actaccgcgt cagttggcgt gtcatcgcca ggtagagtaa ttttcggccc cccgtccaac
     2461 atgcctgtgc gttgcaatat ttcgcgcaac gccggtacgt cgttactcca ctgccctggg
     2521 cgcaacgttg ctttgccggt cagttggggc cacggtttgg tgtcactgag taaggccagt
     2581 aacgattcat gcatcgccgc atattgcgga tgctgcggtg ccagtcctgc aacaaacgta
     2641 ggcaattgac ctttatccag cgccagctgc cattggttaa tcaccgagag cggcggcgtt
     2701 gcaagtgcat aaggtttact gctatatagc cagcgagtgc ctttgaccgg aatatttgca
     2761 atgaaatgga gatagcccat catcgcatca gagagcacta cgtcgcgtgc catcccgtta
     2821 acaccaggat cggtcagtaa ctctacccat ttattaaact gcggctggaa accggcaatc
     2881 gccacctctg ccagctgttg ctggaaggct ttaacagcat cacggttttc ccacatgggt
     2941 tgcatatcgc gtgcggcata caacagttga agctggttaa gataaaccgg cttgtaacct
     3001 gcgggtaatt gagattcgat ttgcgtgcgg gctttttctg ccgcaccttc aggcaatggc
     3061 tggatccccg ccattattgc cgtggcttgc gcctgcggca gtgcctcgcc ctgttcactg
     3121 acagccaccg ggctgtcgcc agggattact tcaggctcat cggcctgcgc attgaacagt
     3181 ggagcgaatg ttacggccag gcacaaactg attgccgaca gccgacgacc acacatcata
     3241 ttaagcaaca tcccttgccc cctgttttta tcatactctg aatagccagg ctgttatttt
     3301 acgacttgcc tgtacaacgc caatactcac gaaagtatga cgagcaagcc tgttactcat
     3361 caggcttatc ttcagtatat aaacgccgat acgtttttac ataacctaat gtaaagaagt
     3421 ttaaagaaaa gcgcgccgta atgtgccact tttttagtgc agaaacggag ttttcggaaa
     3481 aagaaaaggc ggcattgctg ccgccttaat tttaactcgc ctgagaaggc gcttcgtcac
     3541 ttcctggaag cgtttcaggg agttgcggcg caaatcctcg caggccgacg acgtgaacgt
     3601 gttcggtatt ctggaagact ttacgcacca gtttataggt ggtgcctttc tccgggctga
     3661 tattttccgg cgctgcgatg atgagctgca tttgcaaacg ctcacacaat tcaaacagcg
     3721 tggcgataga acgagcatcc agtcgcgctg cttcatcgag gaacagcagg cggcaaggag
     3781 agatatcttt accgcgcagg cggcgagatt catcttccca gctttgtacc accatcacca
     3841 gaatcgacat accggtacca atcgcctcac cggtcgacaa tgcaccagac tctgcgcgca
     3901 accaaccatc ggaaccacgg ttaacctcaa cttccatttc cagatagttg cggtaatcca
     3961 gcagttcttc gccaatggtc tgcggcgtgc gctgccccat atcaatctgc ggattaagac
     4021 gctgatacaa tttcgccaac gcttccgaga aggtcagacg gttgctgtta aacagatcct
     4081 gatgctgctc gtgctgttca gagagcacat ccagcagcat ggcgtgcgtt tcacgcacgt
     4141 tcacgttgag gcgcacgctg ttcacctgac caaacgatac gttctgcaac ccctggttga
     4201 gcatacggat acggttctgc tcgcgctgaa tggttttgcg aatgatgttc gccacgctgc
     4261 gggaactgat cgccagtttc tgttcacggg aggttaattc ttcggtcaga cggctaagtt
     4321 cgatctccat ctgttcgata gcttccaccg gatcatcggt acgaataata tcctgacgaa
     4381 tacgttcacg cagatgctga taaaccgcca cgaagaactg aattttacgc tccggacgtt
     4441 tcggatcttc cgacatgcgc agcacgtcgc gcagatgttc gttatccgcc accgccagac
     4501 gcagcgcacc caatgcctta tccgacatag agcgcaaatc atcagcggag agataagcca
     4561 gctcacgacg gtgtaagcgg cgctcaacgc cgttatcttt caccatgcgc atcaccgcac
     4621 accagcccgc tttggcggtc actacctgct cgcgcatctc aaagtaatcc cgctccagct
     4681 tgcgcagttt gcgggtcagg ttgtccatct ccgcttcgca gaaggtaagc gctttttcca
     4741 gttgattgcg gcgtgaacgg ttattgctca gttgcgcatg cagctcgtca cggcgaatac
     4801 gcgcccgctc ttctgccccg ctatcagcac gcacgccgat atcctgcaat tcacgttgca
     4861 gatcgttgag tagctctttt ttggtgtcgt aagaactttt cagcgaagcc agcacctggt
     4921 tgtactgact caactgcgct gcgtgaccgc gcaacgcttc gcgagcgcgg gtacgctccg
     4981 cttccgcctg ttccagacgt tcacgcagtt tttcgttgag atcgctgtta ccgctaagca
     5041 tttctgccga gtcagagtag ctaaagtgcg cacgacgctg caccacttcc gtcagggcaa
     5101 acgcctgctg acgagcatcg cgctgcatct gctgagagta cgcgtaatct tcttttaact
     5161 gttcgaactg ttccgggtcg ctctgcaata ccgaaacgat cggttccagt ttcgccagtt
     5221 gattgccaaa ctgctgaaca aaacgcgcgg cttcctgggc ttcatccaga cgttcgcgga
     5281 tttcatcgac gcgatccgcc aggctatcat cagccaacag gttgagacgc ggcagaatgc
     5341 ggttcagcgc cgtaacgccc tctttcgcct gctcaaactg aatacgctgc tgctggttat
     5401 cattttcatg attacttaac gcccgctcca gttcgacgcg acggctgttc agttgacgga
     5461 tttctgcttc cgggtcagac tcaaacgcaa ccgccagatg actgccgata aagcggctga
     5521 acgcctgatg cagacgctga gttttctgta catcaaagga gagcgtggcg aagcgttcgg
     5581 aaagcacttc acgctcggca tggaggcttt caatacggct ttcacgcgca gcacgaccaa
     5641 acagcggcac ttccgggaaa cgtgaataac gccactgacg atcggcgatt ttcaccacta
     5701 ccgctttttc cagctcatca acgctgaaca cgctgtcatc gaatgactgc ggatctcctt
     5761 cgatcagata gagatcttcc gggcaatcgg tcaagccttc caggtgttca gttacctgtg
     5821 acagatctgg caccacgatg gcgtggcgtg acgggccata cagcgctgag aagtacggcg
     5881 catcttccag gctaacgtcg tcataaattt ctgacagcag cacaccacca aaacgctccg
     5941 ccagcgcgtt cagacgctga tcttcagagc cgccaggctg gcttaaacgt tcgatctctt
     6001 catcgacggc gtttttgcgc gcgcccactt catcgcgttc aacaatcgcc tctcgctcac
     6061 gctccagcaa ctgttgcaga tattcggtga cgtcctggct ggaggtaaac tcttcgccgc
     6121 actgttcgct caactggttg agactgtttt gcgctgccag ccaaaccggc gcacgctgca
     6181 tcaaactctg aatgcgagac tgcagctgtt cctgctcctg gcgcagtgcc atgcgctctt
     6241 cacgggcgtt agacacgcta tcggaaagag aggcaatgcg tgcttccagt tcctgatgca
     6301 gggcttccag ttcggcgata tcaaaattct tgccctgacg tttgcagaag tctgccagca
     6361 ggcgttcggc ttcttgctgc tcgcgcagac gctgttccag ttcacttaag cgcatccgca
     6421 acggttgaac ctgctctgcc aggtgacgct gatcgacccc ttcgcgcaat agttcgcgag
     6481 cgacatccca cgcctcgtta cgcgccagcg ggccgttgat tgccaccacc agctgataag
     6541 cctgctcaaa ctggctgtgc gcggtttgcg ccatgctcat tttctgctca agagagagca
     6601 ttttttcagt cgcttccagc tctttcgcct ggaaggtttc cagccattcg gcggcgctgt
     6661 cggcggttaa gtccggcaga tggcacagtt ctttggcgcg attaagcgca gcaatcgcct
     6721 ggttatactg gatcgcgcgc gtttgctgga cgtccagcgc ctgctggtag tcagcaagct
     6781 ggcttttcag ctcgtccact tccagttcgg cagcttccgc gcgagcctcg ttctcttcct
     6841 ggcgttcgat ggcttctgcc accacttcgt tttgctcttc cagacggatc tgcagctcat
     6901 cgagatccgc ttcgtagcgt tcgatcttct cctgctgacg cagtgcggtt tgcaccaggt
     6961 tcaggtgatc gctggccgcc tgataatccg cttccagatc gccttcggca ccgttgtgct
     7021 ctgccagctc acgcgccata tcgacgtgtt tgtactgctc agccgccagt tgctgacgcg
     7081 aagtatgtaa ctcgcgacga aactccaggg ctttgtcgag atggacacgg cgctcgttgg
     7141 cgtgacgcat gtagtccgcc gccacgtagt tggtagcttc gctgatcaga tgcttaaaca
     7201 ggtcacggtc cgactgggtg acacgaatcg cttccagcgt catgcggttt tcacgcagcg
     7261 ccgcttccat gtcctggaac gctttacgca cgccgctgtt ttccggcaac aggtagtcgc
     7321 gcagagaacg ggtaatggca ctggagatcc cgccatacag cgaagcttcg atcagacgat
     7381 agaatttgct acggtcagat gcggagcgca gacgacgcgc gatgatgccc agatcgaaca
     7441 tcagcgagtg gtaatcggta atggagttga actgtttaaa ctgcacgcct tccatcgcct
     7501 cgagcttgtc tttcagctcg ttaagcggca gcacgcgcgc ctggcgttcg ttcagggttt
     7561 cggtcaccag ctgtgtcggc tgcaccgaca tcggcagtcc ctgaatggca aatggtttga
     7621 tatccacttt acgatcgcgt ccggcaacct gttgcagacg cacaccgacc accacgcgct
     7681 ggtggcgcga gttaatggtg tcgagcatcg aataacagac acccgctttc agcttaccgt
     7741 gcagaccttt atcgcgcgaa ccgctggtgg ccccggcttc cgtagtgtta cggaaatgca
     7801 gcagggtcag gtcggggatc agcgccgtaa cgaacgccgc catggtggtg gatttacccg
     7861 ccccgttacc gccagaaagc gtcgtgacca gctcgtcaag gtcaaaagtt cgggcaaaaa
     7921 agccgttcca gttaatcagc gtcagtgagc gaaatttacc gcgttcaatc attattcttc
     7981 ctctccgcta tctggctgat tctcttcggt ttcatcgttg agttgcagat gattttcaat
     8041 cggcattgct tcgccatcac gaatcaggcg acgctgtgct tcacggggat cgtcgccagc
     8101 acgcacatcg gctccgaagc ggaacaccga ttcggtaatg cgaaacttgc tgctgtcgtg
     8161 gcccataaac cacaccatgc ctaaacgacg cagacggttg agcgaagaac gtactttctc
     8221 ctgcaacttc tgacggtcaa cgtctgaacc ggttgaacgg ttgttcacca gtttcagcag
     8281 ttttgcttca tcggccaggg tgagcagttc gtcgtacagt tcctgctggg tgaaaatccc
     8341 ctcattcgcc agccgttccg ggctgagata gagataacag aggattttcc cgaccatcat
     8401 atccagttcc gacaagacgg aacgagggat cagcgtggtg gaacgtgggc gtaaatagaa
     8461 gaacccttct ggtgcgcgaa taagctcaac gttataacgc gcgtaaaact cttccagata
     8521 ttcctgaaaa tccatcagga atgcatgatt atccagttcg tcgaggccaa tatggcggcc
     8581 tgaacgtaag gcgctgtcca gcgccggaaa taacggattc gccagcgcct gcgccagctt
     8641 aaccggcatc acttgttcaa tatttgtcga tgacatgcgc ctgtaccttg gctccgtaat
     8701 cattaatcgg ctgccatttc gctggcagtc cggtgaaatc tgcttgcgct acgccaagac
     8761 gtaccgcctg atcaataaca atacgcgcaa cgtcaaagtg acgtgcacgc ggatactgtg
     8821 acagatattc gcgtaccacc agaccaagat ccagcggcac ttgtctggtt ttgtacacgg
     8881 caagttgttc ttcgatgatc gccgccagct gttcgcggat ctcgttaaac tcttcgtatt
     8941 ccagatcctc aggaagttcc ccagtcactt cttcatcgcg cagtgccatc tcttcgtcac
     9001 gcatatccag cagacgatcg gcattggcat aagttagcgc ccacggctca tcaaaatagg
     9061 tttgtaccga ctgacgtaac cgctgagcaa agacgcggtt tttatccata tcgatcgcgg
     9121 tacgaataaa tttgtgtacg tggcggtcgt agccaatcca caagtcgatg gattgctggc
     9181 cccaactgat aatacgatcg agtttgctct gcagatcgaa caccagacga tcgacgaaat
     9241 gcagatcgtc atgggtcatc gtcgcatcct gaatgcgcaa cagattagcc tgcaatttgt
     9301 cgcctgccgc ttccagcgta tcctgcaatt cacgcagcgt cccggaagtt tccgaaagca
     9361 acaattcaca gctggaaatc gccgcccgcc agtctttgtt cagcaactgg gcgatatcgt
     9421 ccttcacctg ctgctgctgt tcgtccatca gacgttgcgt caggtcgata ctgtcgaaaa
     9481 tttctgctac cgaatatttc agtggcgcat agacattacg gtgccagtga aattcatcac
     9541 cgccctcttc ggcggcatct gctgcgcgtt tgagctcacc cgccacaatc gacaactgca
     9601 tagaaagacg cagcgtagaa aactcgcgct gacggatgta gtagtcagta atgccgatgc
     9661 cgagcggcgt cagacggtaa attgcgttcc cttccgcctg ctcgctggta aagcggttca
     9721 gcagacgttg acgcaccatg tcgttgatcg cgttattggc gcgcacgccg atggtttcgc
     9781 tggtttgctc aaacgcatca ctcacatggc ggaatgcatc caccagctcg ccttcactca
     9841 tctcaccatc cagacgctcg ccgttcagcg tggcaaccgc cagcagaaaa gagagtcggt
     9901 ctaccggcag cgagatggag aagtcatttt ttctggccca ggcaaccagt tcggggactg
     9961 tctgggaaaa ttcactcata ctttatcctt gctctgcggt ttgcgcgcgg tgacatgaat
    10021 ataacgcccc agggtaatat acggttcctg acggcaataa cgcgtttcta attcaagtaa
    10081 tgcttcatag cagtcgcgct gctggtgctt ctcgcgcaga taatcatgaa acacgcgaac
    10141 gcctgtctta cccataattt gccaaccagc ttcttccagc cacagataaa cctgcgccgg
    10201 gtcgcgtgga taatctggcg aaagcgtccg ttttttcttt ttcggcattc ccgcctgcac
    10261 gtaatcaaaa ttcccggcga ccatgttatg catcaacaaa ccatgcgcat tgtagaacat
    10321 taacgacaac acgccgcctg gacgcaacac tgaccacagg gtctgcaata cactgcgggg
    10381 atcggccacc cactcgagca ccgcatggaa caatatcaga tcaacgggcg tttccaaatg
    10441 cgaagcaaca tcctgagcgg cgcaatgtat aaattgcatg ttgtcgctca caccttttgc
    10501 ttctgccgcc tgttttgcgc ggtcgatcat ctgcgcagaa agatcgcata aaatgacctg
    10561 atgcccacgc tcggccattt tgattgcggt ctgcccttct ccaccgcccg catccagcac
    10621 acgcagtttt tgcgggccca tttccgccag cacgcgatcg agatcctgcc acagaatagc
    10681 ctgtcgaagc tgccctttgg tggtgccgta aatgttacgg gaaaactttt ccgcaatatc
    10741 atcaaaattg cgatcctgca ttgctttctc caccggttgg ctaagacgtt ttctgataaa
    10801 gacgctattt tgtcacagcc acggcgagaa tgaacccgtc tggtgtaata tcgctggcga
    10861 tcccttgcta tatggttaaa aaaggaacca gaaaggatgc tttttacact gaaaaaagtg
    10921 attggcaata tgttgctgcc acttccgctg atgttgttaa ttattggcgc tggcctggcg
    10981 ctgctgtggt ttagccgttt tcaaaaaacc gggaagattt ttattagcat tgggtggctg
    11041 gcgctcttgt tattgagttt gcaaccggta gccgaccgcc tgctgcgtcc catcgaaagc
    11101 acctatccga catggaataa ttcgcaaaaa gtggactata tcgtggtgct cggaggtggt
    11161 tatacctgga acccgcagtg ggcaccgagc tctaatttaa tcaataacag tctgccacgc
    11221 ctgaatgaag gtattcgctt atggcgcgaa aatccgggat caaaactgat cttcacggga
    11281 ggcctagcaa aaaccaatac ggtgagtaca gcggaagtag gtgccagagt tgcgcaatcg
    11341 ctgggcgtgc cgcgcgagca aattatcacc ctggatttgc caaaagatac cgaagaagaa
    11401 gctgcggcag tgaaacaggc gattggtgac gcccctttcc tgctggtgac ctccgcatca
    11461 catctgccgc gggcgatgat cttttttcag caggaagggt taaatccgct accagccccg
    11521 gcgaatcagt tggccatcga ctcgccgctt aatccgtggg aacgggcaat cccttctccg
    11581 gtctggctga tgcatagcga tcgtgttggc tatgaaacgc ttggccgcat ctggcagtgg
    11641 ctgaaaggtc cgtcaggcga gccacggcaa gagtgatttt gatgccagat cgaagttgcg
    11701 ccgactaaat cgtccggtat taaccagttg cgcaacttca tcccataaca catacaacca
    11761 acgccgccag atgaacgact ccgccactgg cgcgcgttgc aggtaactcc agagcaaatc
    11821 ttccgccaga gaattatcca ttagtcggaa cagttcgtat tctcgcggtg cccaaagcat
    11881 tagtcccggc ccgaccatcg ccagtaactg atcgctgcgc gaatctttca gcatgctgcg
    11941 taaacagaag ttaccgtgaa tcaacacaca attgtcgtta aagccttcaa ataatgccgg
    12001 gagacattcg cgagtgcgaa acaggatccg cttatcctgc atcgtcagac cggtgttatt
    12061 gaactgattg agcgtggtcc atagcacttc aacatgttgc cggtaccatg agggccagaa
    12121 attttcctga gtattgtcga ccgcgccgac gcaacctcga ctgtcctgac ggtgccaggc
    12181 cagtaaggct tcaacgattt ggtctttgag ctgttcccag cgttctggtg ttcgggctgg
    12241 tgcctccacc gaaacaccac gcatccgctc cagtagcagg acatcagggc cggggtgttc
    12301 ttcatgcgtc atcacaccat aaatagtcgg catacggaca gtcccacttc ttgccagcat
    12361 ggtggttttc catgccagtt gtcgggcttt tccgggcgta ctaaagcttc ttgccattaa
    12421 cggcattggg tttccctggc tgtcatacaa agcccacaac gccgtatccg ctttttcatt
    12481 gacgcactca atacgactga gtttttcgcc cagtaaatgg cttaattcgg cacgcagctg
    12541 ttccattcag attaccctca taaaagctac tgctttcata atgagcgcca atataaaaga
    12601 tgtcacctga atgagatcaa ttgttgaaaa gaaaaattga ggaaaatcgt aacggtacga
    12661 cactcctccc aaaattggct gaagtgtcgt gaagtgaaat tagcgcattt cagcgcgaac
    12721 gcgctcaaga tcttcagggg tatccacacc tgtgccagga acttcatgcg caacagcaac
    12781 atggattttt tcgccgtacc acagaacacg aagctgctct aacatttcga tgtgttctaa
    12841 cggacttgcc tgccagttga cgtaacgacg gataaagcct gcacggtagc cataaatacc
    12901 aagatgacgc aggaagttat cgccaacggt ttcaaggcct tctgcaaaac gatcacgatc
    12961 ccaaggaatg gtggcgcgtg agaagtacag tgcataccct tcagcgtcga gaaccacttt
    13021 caccgcattc gggttaaacg cttcttccgc attgcggatt ggcaccgcca gagtcgccat
    13081 acccacctga cgctgagcga ggttatcagc aacctgacga atgattgtcg cagggatcat
    13141 cggttcatca ccctgcacat taacgatcac cgtgtcgtcg ctgaacgcgc atttttcgac
    13201 aacttccgcc aggcgttctg tccctgactg gtgatcggcg cgcgtcatac atacttcacc
    13261 gccagcggct tcaacggcgc gggcgacatc ctcatgatcg gttgccacga tgatgcgatc
    13321 ggcacctgat tcgcgagcac gttcaagaac atgaacaatc atgggtttgc cattaatatc
    13381 aaccaatggt ttaccgggca gacgcgttga cgcgtagcgc gcgggaataa tgaccacaaa
    13441 actcatgatt tactctcatc agcagtcagc acgcgggctt ccgtttccag caacactgga
    13501 atgccatcac gcagcgggaa agccaggttg tccagcttgc aaataagttc ttgtttttcc
    13561 tggttatacc aaagttttcc gttgcaaacc gggcaggcaa tgatttcaag cagacgatga
    13621 tccatagttc ctccagatgg atcgggttat gaatgcataa atcttatcat aatcatttat
    13681 gcgacggggt ctatttccca gccagtgcgg caatcagtaa agagaccttg cgggcagcgt
    13741 cctaatgtca cccgcgttgc ctgctgccag ctcgcgaaat cagtaatcgc ctgacgtaac
    13801 cctttttgca gcattgtcgt tggtttaata ccttcctgta accacagaga gataacttca
    13861 aggatgcctg tctggcgatg cattttggca tccattcgcc caactaattg cccacgatgt
    13921 aataatggca gaacaaaata gccatactgg cgtttcggcg ctggggtata gcactccagc
    13981 cggtagctaa aatcaaaaag ctgctctgcg cgtttgcgat cccagacaac aggatcaaaa
    14041 ggcgaaagta ccgcgctatg cgttgcagtg agctttccgg ccagcgcacg ctcgagtagc
    14101 ggcagcaaat cagcatgcag ccaaagattg cccaattttt caacatgcac agcaatgatt
    14161 tgctgctgtt cagccctcgc ttcgcgccac gccgccagtg ccggacgttt cagccgatag
    14221 taatctgcca gccactgttc gcggaatatt cccaggctac gcgcactgtt atccagcatg
    14281 atgatttctg cttctgtttg cgaaacgaga tcgcgctcat catcccagtc aggcatgaca
    14341 cggtgggtta aatcataaac gcgctggaag ttgcgccgtt caatcaccat cacctttccg
    14401 gcagtaaata aaccttccag atgccgttta tgcggcttcc attcccacca gccgcttgca
    14461 cctttacgag gatgctcaaa atcggctgaa cgtaccggcc ccttatcatg aatatgctga
    14521 attaactgtg caatttccgc ctcatgttcc tgcatccagg cgtctttgta tttccagccc
    14581 attttttcag gtgccagcat gcggtggcga ataagacgaa agtcgctacg cggcataaag
    14641 caggcttcat gcgcccagta ttccattaat tcgccacgcg ccagagactc atccagccac
    14701 tgggcaggat aatttcccag acgactgaaa agcaccagat atggactacg ggcaacaata
    14761 ttgatggtat cgatttgcag caaggacatg cgggagatcg ttgccggaat atcctccaac
    14821 gacgctcgac ggcggggttt gtttaacagg ccttgtgcgg caaggtgaag attacgcgca
    14881 tcagcaaggg agaggtgcgg cagcgacatt catgactcca tcaatcgaac gctgccgcgg
    14941 cgtaactagt tgccagaagc cagcgaggtt agttgcgtaa gcagtttcgc tggttcatca
    15001 cctgaaagct gtgcgtctac aggcaaatac caccaatttt cttctgcaaa ggcccggcat
    15061 ttcaccgcat ctttttcagt cattaccagc gtttgcccgg cgcttaccaa cgcactgaca
    15121 tccgcatggt tcaaagactg atgatcggcc agcggtacac atttttccgg ttgtacgcca
    15181 cacatcttca gcgtggcaaa aaagcgcggc ggatgcccaa tccccgccat cgccactaca
    15241 tgttcaagct gagcaacgtc acaacgcgta ccggtacgta aattcaccgc ctgacccggc
    15301 agcagatgca tggggatttc accgctgcga gggacaccgc cgttgacgat taccgcatca
    15361 accgacttta agcgccccgc tcgctcacgc attggccccg ccggcaacca ccagccattg
    15421 ccaaagcgac gcacaccatc aataacgaca atttccacat cacgcgccag acggtaatgc
    15481 tgtaaaccgt cgtcggttac gatgatctgc acatcagggt gttgcgccag aatggctttt
    15541 accgcatcag aacgaacggg agaaaccgca acaggcgcat cagtacgttg ataaatcaac
    15601 acaggttcat cacccgcctg tgctgttgtg gtatctgccg acaataacag cggataagat
    15661 tcagccttac caccatatcc ccgcgatacg acccccacgc gaataccgcg ctgttgcaac
    15721 tgttccacca gccagacaac gaccggggtt tttccgttgc cgcctgcggt gagattacca
    15781 accacgacaa ccggtacggg ggcacgccag gcgcgcttca gttttagttt atagcaaaga
    15841 cggatcgcgc cactcaccag gccatacaac caggagagtg gcagcaatag ccgccacaaa
    15901 ggggattcac cagaccagat tttttcgatc attggccaaa ctgcattttg tgaagttgcg
    15961 cgtaaacgcc gcggtgctca agcaaatcgt tatgcgtacc gcgttccaca atgacaccat
    16021 cctcgacgac cacgatttcg tctgcctttt caatggtaga caagcggtgg gcaatcacca
    16081 gagaggtacg gtttttctgc aactcatcca gtgccgcctg aatcgcacgt tcggattcgg
    16141 tatccagagc cgaggtagct tcatccagaa tcagaatcgg gctatcacgc agcaaggctc
    16201 gagcgatagc aatacgctga cgctgaccgc cagagagcag cacgccgttt tcaccaatca
    16261 ctgtatcgag accgttatcc atcttattga tgaagtccat ggcgtaggcc atacgcgccg
    16321 cttcttcaat ttgctcacgg ctgtactgtt cagtccgtgc gtaagcaatg ttgttagcaa
    16381 ccgtatcgtt aaacagatgg acattctgcg acaccagagc aacctggtta cgtaacgacg
    16441 ccagggtata ctcgcgcaga tcgtgaccat ccatcaggat ttcgccttca tcaatatcgt
    16501 aaaaacgagt gatcaggctg gcgatggttg atttacctga accagagcgt ccaaccagag
    16561 caaccgtctt ccctgccgga attttcaggt tgatgttacg caatgcaggt acgtcacgtc
    16621 ccggataagt aaaggtgaca ttgcggaatt ccacgtcgcc agtcgcacgc tcgatcacgc
    16681 gcttaccttc gtctttctcc tgctcactgt ccagaatggt aaacagcgtt tgacaagccg
    16741 ccataccgcg ctggaactgg gcgttaacgt tggtcagtga tttcagcgga cgcatcagtg
    16801 caatcattga agagaaaaca acggtaatcg taccggcagt caggctatcc atgacacttg
    16861 ggaagctcgc cgcatacaga acaaacgcca gcgccaaaga ggcgatcagc tgaatgatcg
    16921 gatcagagat ggaagaggct gaaaccattt tcatcccctg aagacgcatt cggttgctga
    16981 ctttatcaaa gcgtttcgtt tccacttcct gaccaccgaa aatcaatact tctttgtggc
    17041 ccttcagcat ttgttcggcg ctggtggtca cctgccccat ggtgttctgc atgtttttac
    17101 tgatgttgcg aaaacgcttc gatactacgc gaatcgcaat cgaaacaatc ggtgccagca
    17161 caatcaaaat gatcgacagt tgccaactgt aatagaacat catgatgaac aggccgatga
    17221 ttgacgcacc ttcacgcaca accgttatca gtgcgccgga agaagaagaa gcaacctgtt
    17281 cggaatcgta ggtgatacgt gacaacagcg tccccgttga ctgtttgtca aaaaatgaaa
    17341 ccggcattcc catcatgtga ccaaacaggc ggcgacgcat ggtcattacc acctttcctg
    17401 atacccagga gatacagtag ctggagacat agctggtgat accacgtaaa atcatcagcc
    17461 cgatcaccac cagcggcatc cacaccagca cggagcgatc tgttttacca aagccatcat
    17521 caagaagtgg cttaaggagc gataacatga aggtatcgct ggctgcgttg aggattaacg
    17581 ctacgcccgc cacgatcaga cccgctttaa aaggcgcaat ggttggccac agtcggcgga
    17641 acgtctgcca cgtagagaga tctttgtcgt tatgcattca aaaaaccagc atttgttgaa
    17701 atagccgcat attctacccg ttatccactg gcacgccaaa ccactgatga taccaacgag
    17761 gtaaaatttg ctctctgagg ctgctaatcc gccagccttg cgctgaaaaa ttgaccgtca
    17821 cttgaccctg atgtggagta tcaagccatt gatatccttg ctgttgatag cgatgcttaa
    17881 ctttattaga gggcaatcgc catgcgttat agcgcgatgc cgatgcgagt gccacttttc
    17941 cattcactcg ctgaattaat ggcaacgatg atgaggtatt actgccatgg tgaggtacct
    18001 gaagcaatgt tgtctgcact tgctgccagt aacggcttag catcttttgt tcagcgggga
    18061 cttcaatatc accggttaga agaatgctat tcgtcccgtc atcaacctta accacacagg
    18121 aatggttatt tcctttatcg ttgctagctt gtaaaggcca gtgcacgctg aaacgcaatc
    18181 cttgccattg ccacgcttcg ccacgcacac agggctgatg atgttcccag tttaacggac
    18241 ttctgatcca taacatcggc catgtgtgca atattgagtc cagccctccc cggtgatcca
    18301 gatgttcatg actcagaata acgccctccg gttcaagatt atgccagtgg agccagggga
    18361 taatcagttg ttgcccacta tccccttcgg gccaggccag tcctgtgtca tagagaatcg
    18421 ctttgccgtt tctggctatc actattgcca gcccttgccc gacatcaagc atgtacacct
    18481 gccactcgtc aggtcgaggt ttttgccaca gcggccagca catcagcaag cctacagcca
    18541 cacacattgc tggcaacgtt cgccaggcgt ttaatcgcca taccaccagt aagaaccatg
    18601 gggaaaatga tagccattgc caacgttcag caatgttgat ccacccttct ggcaaactct
    18661 ttaaccccca gaaaagtaaa gccaaagacc ggtcggcaag aaaccataac ccttgctcca
    18721 ggattaacgg cccgcttaaa tgcacaacca tcgcggcgag gatcaacgga accgtgataa
    18781 atgtcaccaa gggaattgct aatagatttg caataaacga ggtcagacta atgccatgaa
    18841 atatcacgat ttgcacgggc ataagcagaa gtgtgatacc cagttgcaga tggatgaggg
    18901 aaacaactgc acgcaatacc ggcggcagtt gccactcagg acagggaaac cactgatacc
    18961 aaaaaatcag tgccgcgacc gcagcggcag agagccataa actttgcgag agaatggcaa
    19021 caggatccat cagcaaaatt gccgccagac aacatatcca tacatcccag ccactccact
    19081 gtcgcccact taacttaagc attccccacg tagcaagcgc caccacggta cggaatgcag
    19141 gaggttgcat tcccgtcaac caggcataaa aagcagcaca gcagatccca ccaattaatg
    19201 gtatttgcca gtggatccag cgcccaggca gaaaaatttg tccactgcga atgagtccgg
    19261 cagccagcaa cgccgcaaaa gcgatgtgca atcccgatat cgccattaaa tgcgccgttc
    19321 cagtatcgcg catgatattt ttgatttctt tggggacgga taaccgttcc cccataccta
    19381 aaccaagaat aaccgcattc cacggatagg gttgcagcgt tgtttgtagt gacgccagat
    19441 actgtgcacg caggctacaa ttcggttcaa taacacttgc ctgcagaaag cggccggtga
    19501 gcggctgatg ctgggcaatg gcataacgct ggctatcaaa gccgccatca ttaagttgac
    19561 catgaacggc acgaactttg agtttcattg accacagttg tccggcacaa accgcttgcg
    19621 gaagatattc gccatacagc acaaggcctg gcgcagggaa tatacgtcga ccttgtagat
    19681 aagtaatttg accgtaatga gtggtcatgc cgtcagtagc agtgatctca acaattgcat
    19741 cctgcgtcgc gccagttaag gtttctcctg cccacaaaat ttgctttgct gataatatgc
    19801 cccaaacgaa aaacagcaac gtcagcgcga tataacggac ggttttaaca gggataaatg
    19861 ccagtacgca ggcgaagaga gtcagaaacg caagggttaa tgtcccaggc aattggggca
    19921 aaatcagcaa cggaaaaatt ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc
    19981 acccctccct gtttatggaa agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt
    20041 gcgttccccg aggcatattc agaagaattt aacgaaaggg tgaaaactgc tcaaaactct
    20101 gcgaggcagc ttccagttcc tgattagaga aatgaaaaaa gcacccgaca ggtgcttttc
    20161 tcgcgttcaa gtttgagtaa aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt
    20221 accaggttta aagtgaggaa cgtattttcc ttccagttct actttatcgc cagtcttcgg
    20281 attacgtccg gtacgtggtg cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc
    20341 aatacgctcg ccctgcgcaa gagtcgaggc catatgctcc agcatctctt ttactgcatc
    20401 ttcaaccgtc ttggcgggaa tgtgcgattg ctgggtggca agtctttcta tcaattctga
    20461 cttggtcatg attcctccgg ttccttaaag gcaaattaag cggctgctgt tgctgcattg
    20521 attaagggcg gccgtagccg cccttagtgc ttgattacag gacgaaacct gcaatctgtc
    20581 aagtaaactc aacaaacttc ggaataaaaa tcccgaagag tcagagaatt actcgccttt
    20641 agctgctttg aaagcttcag ccattgcgtt gttggagaag tttgcatctt cctgtttgtt
    20701 aacagttgcg attgcatctt tctcgtcagc ttcgtctttc gcacgaacag acaggctgat
    20761 tgcgcggttt ttacgatcaa cgccggtgaa tttagcttca acttcgtcgc caacgctcag
    20821 aaccaaggta gcgtcttcaa cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc
    20881 aacgccgtca gccagttcta cggttgcgcc tttagcgtca actgcagtta ctttaccggt
    20941 tacgatagcg cctttcttgt tcagagcaac ccagttgttg aacggatctt ctgcgagctg
    21001 tttaacgccc agggagatac gttctcgttc tgcgtcaacc tgcagaacaa ctgcagcgat
    21061 ttcgtcgcct tttttgtatt cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc
    21121 agacaggtga accaggccgt cgatgccgcc gtccaggccg atgaagatac cgaagtcagt
    21181 gatagacttg attttacctt caacacggtc gcccttgttg tgggtttccg cgaactgctg
    21241 ccacgggtta gctttgcact gtttcagacc cagggagata cgacgacgtt cttcgtcgat
    21301 atccagaacc ataacttcca ctacatcgcc aacgttaaca actttggacg ggtggatgtt
    21361 tttgttggtc caatccattt cggaaacgtg taccaggcct tcaacgcctt cttcgatttc
    21421 aacgaagcag ccgtagtcgg tcaggttggt cacgcgacca gtcagtttgg taccttccgg
    21481 ataacgttta gcgatagcta cccacggatc ttcgcccagc tgtttcaggc ccagggatac
    21541 acgggtacgt tcgcggtcga acttcagcac tttaacagtg atttcgtcgc ccacgttgac
    21601 gatttcgctc ggatgcttaa cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc
    21661 aacgccgcca agatcaacga atgcaccgta gtcagtgagg ttcttaacga tacctttaac
    21721 ttccatgcct tcctgcaggt tttccagcag ctgatcgcgc tctgcgctgt tttcggattc
    21781 gataacggca cgacgagaaa caacaacgtt gttgcgcttc tgatccagct tgattacttt
    21841 aaattcaagc tctttgcctt ccaggtgcag agtgtcacgc accggacgaa cgtctaccag
    21901 agaacctggc aggaacgcac gaataccgtt cagctcaaca gtgaagccgc ccttaacttt
    21961 gccgttgata acaccggtaa cagtttcagc atcttcgtaa gctttttcca gcgtgatcca
    22021 ggcttcgtga cgtttagctt tctcacggga cagcagagtt tcaccgaagc cgtcttctac
    22081 tgcgtccaga gcaacgtcaa cttcgtcacc tacctggatt tccagctcgc cctgggcgtt
    22141 tttgaactgc tcagccggga tggcggactc agatttcaga ccagcgtcaa ccagtactac
    22201 gtctttgtcg atagcaacaa caacgccacg aacgatagaa cccgggcggg tttcgatttc
    22261 ttttaaggac tcttcaaaga gttgagcaaa agattcagtc atgtttaatc ttcaggttta
    22321 tatttaacgt ccacctggct ccatgccgga tggggttgtt taacataccc gctgtcattc
    22381 cattgcaacg ggggtactgc aaattcggtc gcttatgcga gagccaattt ctggcgcgcg
    22441 tattgtagcg ctttttcaat cacttgctca atgcttaagg tggtggaatc caacactaaa
    22501 gcatcggctg ccggaaccag tggcgctacc gctcggttac gatcgcggtc gtcgcgttct
    22561 ttgatctcgg ccaaaaggcg ctcaaagtta acactaaagc ccttctcctg caactgtagc
    22621 attcggcgat gcgcacgttc ttccgaggag gcgtcaagga aaattttcac tggcgcatca
    22681 gggaatacca ccgttcccat gtcgcggcca tcggcaatca ggcctggtaa ttcgcgaaac
    22741 gcgcgttggc gacgcaataa tgcttcacga acgcgtggga atgccgcgac ttgtgaagct
    22801 gcattcgcca cttcctgagt acgaatttcg ccgctgacat cttccccttc gaggatcact
    22861 tccagattgc cattggtcga cacaaaacgt acatccagat gggatgccag cggtaccagc
    22921 gcatcttccg acgcaacatc aacatggtga tgtaatgccg ccaatgccag tacgcgataa
    22981 attgcacccg agtccagcag atgccattgc aacgcttccg ccatagcctt acacaaggtg
    23041 cctttccctg caccgcttgg gccatcaatg gtaataaccg gggcaattgc cgtcatcttt
    23101 atctccttaa aacaggcgta ccgttaacat aaccgcgcgc attatacgcg ccgacgccac
    23161 aaatcgttaa cgttgtgtgc aaaaaccagt tttgtctgta ttcagatggg gatttattgc
    23221 gcaattatag cgggctggca gaagaccagc ccgaaagatg acaatgattt acttaccaga
    23281 ggcgatacga tcacggatgt gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat
    23341 ggactgggtg cgaccgccat ccaggctagc cagtttttca aagctgccaa ccagcccctg
    23401 ggtgctgatg ccacgtttct tcaacagatc aaaggagaaa tcatctgctt cggactcttt
    23461 atcacgggag tatttagcat tgatagcgcc ttctgcgata tcacctaatt gtgagcggga
    23521 aagctgggaa gccacaccgc tggtagctga aatggcatcg cgtgcggcaa cgatcgcata
    23581 agaagctttc atttcagcca gcgagtgacc caacgcgacg tggcccagtt catggcccag
    23641 aacgccttca atttcgttgt cattcatcat gtccatcagg ccactgtaga cacgaacaca
    23701 gccgttcgcc atcgcccatg cgttgacgtc gctggtcata taaaccttat agttgaccgg
    23761 cgtgccatta atattgttac ccaatgcttt ggcgattttt gccagacgtt taccgtactt
    23821 gctggattta cttgcgactt gattgccgct gtccatttcg gcacagccct gattggcaat
    23881 cgcttttgca tcggcatcgc ttaatgttgc tgctttatag gcatttaaac cggagctgat
    23941 agccatattg gtatcaatac cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt
    24001 cgcaatcgcc agcagtaatt tagtattctt catgtga
//
LOCUS       DAOFQD010000068        23759 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.068, whole genome shotgun sequence.
ACCESSION   DAOFQD010000068 DAOFQD010000000
VERSION     DAOFQD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.068"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(67..978)
                     /gene="gsiD"
                     /locus_tag="QBF46_002800"
     CDS             complement(67..978)
                     /gene="gsiD"
                     /locus_tag="QBF46_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDQ6674961.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(981..1901)
                     /gene="gsiC"
                     /locus_tag="QBF46_002801"
     CDS             complement(981..1901)
                     /gene="gsiC"
                     /locus_tag="QBF46_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDQ6674962.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(1919..3457)
                     /gene="gsiB"
                     /locus_tag="QBF46_002802"
     CDS             complement(1919..3457)
                     /gene="gsiB"
                     /locus_tag="QBF46_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDQ6674963.1"
                     /translation="MARAVHRSGLVALGIATVLMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLGSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3477..5348)
                     /gene="gsiA"
                     /locus_tag="QBF46_002803"
     CDS             complement(3477..5348)
                     /gene="gsiA"
                     /locus_tag="QBF46_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDQ6674964.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAIIFQEPMTSLNPVFTVGEQIAESICLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5335..6300)
                     /gene="iaaA"
                     /locus_tag="QBF46_002804"
     CDS             complement(5335..6300)
                     /gene="iaaA"
                     /locus_tag="QBF46_002804"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDQ6674965.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6504..7739
                     /gene="moeA"
                     /locus_tag="QBF46_002805"
     CDS             6504..7739
                     /gene="moeA"
                     /locus_tag="QBF46_002805"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDQ6674966.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTKQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7739..8488
                     /gene="moeB"
                     /locus_tag="QBF46_002806"
     CDS             7739..8488
                     /gene="moeB"
                     /locus_tag="QBF46_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDQ6674967.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8667..9329)
                     /gene="fsa"
                     /locus_tag="QBF46_002807"
     CDS             complement(8667..9329)
                     /gene="fsa"
                     /locus_tag="QBF46_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDQ6674968.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9460..10359
                     /locus_tag="QBF46_002808"
     CDS             9460..10359
                     /locus_tag="QBF46_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDQ6674969.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10365..12797
                     /locus_tag="QBF46_002809"
     CDS             10365..12797
                     /locus_tag="QBF46_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDQ6674970.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGHDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12943..13758
                     /gene="ybiV"
                     /locus_tag="QBF46_002810"
     CDS             12943..13758
                     /gene="ybiV"
                     /locus_tag="QBF46_002810"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDQ6674971.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13910..15175
                     /locus_tag="QBF46_002811"
     CDS             13910..15175
                     /locus_tag="QBF46_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDQ6674972.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15416..17008)
                     /locus_tag="QBF46_002812"
     CDS             complement(15416..17008)
                     /locus_tag="QBF46_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDQ6674973.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17227..18147
                     /gene="ldtB"
                     /locus_tag="QBF46_002813"
     CDS             17227..18147
                     /gene="ldtB"
                     /locus_tag="QBF46_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDQ6674974.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18206..19324)
                     /locus_tag="QBF46_002814"
     CDS             complement(18206..19324)
                     /locus_tag="QBF46_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDQ6674975.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(19321..19788)
                     /gene="mntR"
                     /locus_tag="QBF46_002815"
     CDS             complement(19321..19788)
                     /gene="mntR"
                     /locus_tag="QBF46_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDQ6674976.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            19974..20102
                     /gene="mntS"
                     /locus_tag="QBF46_002816"
     CDS             19974..20102
                     /gene="mntS"
                     /locus_tag="QBF46_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDQ6674977.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20374..21957
                     /gene="opgE"
                     /locus_tag="QBF46_002817"
     CDS             20374..21957
                     /gene="opgE"
                     /locus_tag="QBF46_002817"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDQ6674978.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22006..22521)
                     /gene="ompX"
                     /locus_tag="QBF46_002818"
     CDS             complement(22006..22521)
                     /gene="ompX"
                     /locus_tag="QBF46_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDQ6674979.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22574..22639)
                     /locus_tag="QBF46_002819"
     CDS             complement(22574..22639)
                     /locus_tag="QBF46_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6674980.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            22874..>23759
                     /gene="rhtA"
                     /locus_tag="QBF46_002820"
     CDS             22874..>23759
                     /gene="rhtA"
                     /locus_tag="QBF46_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDQ6674981.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         5756 a         6497 c         5954 g         5552 t
ORIGIN      
        1 caacacttgc ttagggcaat aatttcctgt gaggtgatta ccctttcaag caatattcaa
       61 acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcgc ccaacaaatt
      121 gaacgccagt acggtcagaa aaatagccag ggccggaaaa acagcgacat gcggcgcgat
      181 aaccatatcc gctcgtgcct cattgagcat tgctccccac tctggtgtcg gcggctgcgc
      241 accgaggccg agaaatgaca ggctggcggc agagataatc gaggtgccaa tgcgcatggt
      301 gaaaaacacc acgatagacg agacggtccc aggcaagata tgacgcaata aaatggtcat
      361 atcgctggca ccaatactgc gtgctgactc aataaaggtt tgctgtttca acaccagcgt
      421 gttgccgcgc accagtcggg caaacgcggg gatggaaaaa atggcgactg cgataatcac
      481 gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa tacccgggaa
      541 ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc caccagcctt cataatatcc
      601 agccaacaag cccagcaacg tcccgatcgc cgcaccgata aacacggcaa acacgcccgc
      661 cgccagcgag atttgcgcac caaccaggac acggctgaaa atgtcgcgcc ccagtgaatc
      721 gacgccaaac cagtgctgca aagaaggtcc gttattcagg ttgtcataat caaaataatt
      781 ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc accacaatca ataaaataac
      841 gaataatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga attcatgcca
      901 cggtgtacgc acctggtcag gtttgaccag tggcatggcg tttaacaccg cctgacgtcg
      961 ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc ggcgtaaagc
     1021 acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat ttccgcctga
     1081 atcaccgggt aatcgcgcat ttctacggag tcaacgagta agcgtccaag tccgggccag
     1141 ttgaaaactt tctccaccac gatagatcca ccgagcaaaa agccaaactg taagcccatc
     1201 atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt tgaggacaac ccaggtttcg
     1261 ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacgtc gacaaatgac
     1321 gcacgggtaa agcgcgccat cacggcggcc actgccgcgc cgagcgtcag ggagggtaaa
     1381 atgtagtgct gccaactgtc tgctcccaca gtaggcagcc agcccagttc aacggagaat
     1441 acctgaatta aaagcatccc cagagcaaat gccggaaacg agatccccga caccgcaatg
     1501 gtcatactca atcgatccgg ccaacgatta cgccagacgg cggcgataat tcccgccgcc
     1561 ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt tggcataaag
     1621 cggctggcaa tctcatcggc aaccggacga cgcgacacca tcgacaggcc aaaatccccc
     1681 tgcacagcat tgctgatata gtgccagaac tggtgataca gcggctgatc caaccccagc
     1741 tgctgacgca ccagttctat aacctgcgca tcagcttcgg gcccggcaat caatcgcgcc
     1801 ggatcgccgg gcagcatatg gacaaataaa aacaccagca ccgagacgat aaacagcgtc
     1861 ggaatcaacc ccagtaatcg tttgataacg taattaagca ttccactccc tgcgttgctt
     1921 attgcaaatc cgcgtcttca aagctgaagc cggtgtctgg catgatccaa aaaccggtca
     1981 ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac ggcgattctt
     2041 gccagatgat atcctgcgcc gccttatata agcgggtctt ttccgccgga tcattcgttt
     2101 tcagtgcctg agccaggaag tcatccacct gtttattgct gtaaaacgcg gtattaaaca
     2161 gcgtcggtgg ccagttttgc gaggcaaaca gcggcgatag tgcccagtcc gcttcgccgg
     2221 ttgaagccga ccagccagtg tagaacatcc gcacgccgct ctctttttgc cctttacctt
     2281 caacttctgc cgcccgctgt ccggcatcca tcgcggtcac ctgggcttta atcccgacct
     2341 gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg ttatgtgacg
     2401 accacaacgt ggtactgaaa ccgttgggat atcccgcctc tttcagtaat tcgcgcgctt
     2461 tcactggatc gtaaggccac ggtttataac tttgcgcgta ggcgatactt ggcggtacca
     2521 caccagtagc tggcgttgca tagcccgcaa aggcaacttt caccagcgcc ggacggttaa
     2581 tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa cggcttttgc gtcacgttca
     2641 tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt ttgtttttct
     2701 ccagcagtgt ggcctgctcg tacggaatgg ggaaagcaaa ctgcgcttca ccggtttgca
     2761 gcattgccgc gcgggtgttg ttatccgcca ccggacgcca ggttatgctg cccagtttgg
     2821 gcaatcctgg ctgccagtaa cccgcaaatt ttttcacctt cacaaaatcg gtctgattcc
     2881 aggtatccag ttcatacggc ccggttccca ccggatgaaa accaatctcc ttgccatatt
     2941 tttccagcgc tgccggtgaa atcatcgcgg tcgccggatg ggcaagaata ttaataaacg
     3001 ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg atcgatcgct tccgttttag
     3061 caatattctt atacaggtta tagcgtttaa gatgattcgc cggatcgctg gcccggtcca
     3121 gattcgcttt caccgccgcg gcgttgaaat cggtgccatc ctggaattta atgccttccc
     3181 gcaatttcac ggtgtaagta atgccgtcat cggaaacggt ataactctcc gccagcacgt
     3241 ttttcagttt catctcttta tccagaccga acagcccctg gtaaaacgat ttcgctacgg
     3301 cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt ggtgaaattc gatcctaccg
     3361 ccaccaccac atctttggca gcgaatgcac aagatgccat caacactgtc gcaatgccca
     3421 gcgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc tgaatgttat
     3481 ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg cccgacgcat
     3541 tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa tattgctggg aagatcgtcc
     3601 gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac tgccgccagt
     3661 aatttacgcg tataaggatg ctgcgggttt tcgaagaccg cgcgccgtgg accaatttca
     3721 acaatttgcc cgagatacat caccgccaca cgatgactaa tccgctctac cacagccata
     3781 tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag caacaagttg
     3841 ataatctgcc cgcgaataga aacatccagc gccgaaacgg cttcgtcggc aatgatcact
     3901 tttggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc gccggaaaac
     3961 tcatgcgggt aacgccaggc atgttcaggt aacaggccca cgcgctccag caaccacgca
     4021 acgcgtgcag ccgcttcttt acctggcaat aaaccgtgta cacgcagcgg ttcgataatc
     4081 gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa aataaactga
     4141 atatcccggc gtaatgcctg aagtttgccg ggtgacaagg tatcgattcg ctgaccgtta
     4201 aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc ggtagtggat
     4261 ttaccgctgc cagactcgcc caccagcgat agcgtttcgc caggccagag atcaaaacta
     4321 actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct gcgcaaaggg
     4381 aaacgggtga ccagattacg cacccgtaaa acaggttcgc catccaccac cgttttctgc
     4441 tcgatggggg gttcctgttt cgctggatgt tcaagcgata tcaatgggaa acgtcgggga
     4501 taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc acgggtgtaa
     4561 ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg tttccaccgc ctcgccctga
     4621 tacatcacca gtacccgatc ggcaatctct gccaccacgc ccatatcgtg cgtgataaag
     4681 ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat ctgcgcctga
     4741 atagtgacat ccagcgcagt ggttggctca tcggcaatca gcaccgccgg gcggcatgac
     4801 agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg cggataacgt
     4861 gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca gcatccgctt cgcctcgacc
     4921 atcgcttctt cacgactggc gttctgatgc agacaaattg attcggcaat ctgttcaccc
     4981 acagtaaata ctgggttcag cgatgtcatc ggctcctgaa atattatcgc catatccgca
     5041 ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac ttcacgactg
     5101 cgccgccgca acagcatttt atcgcactgt actaaaccgc ccgcctgttc caacagacgc
     5161 atcaatgcca gcgcagtcac tgacttaccg gagccggatt cgccaacaat tgccagcgtc
     5221 tcaccgcgtt gcagactaaa agagagattg cggaccgcag ctattttctg ctggtcctgc
     5281 ataaaggcaa tattaagatt ttcaaccgcc agcacattac cggcatcaag ttcatcactg
     5341 tgtggcaacg gtgtcccctt tttcgcggta gataccggtg gttggcgtat cgcctgcgta
     5401 gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacgttac cttcatggtc
     5461 gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta ctacccgctc
     5521 gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatgt catatgccgc
     5581 cagcgcgcgg atgaagactt cgcccgtgcc ggtacaagaa accgccacac tggcattatt
     5641 ggcgtagcat ccggcaccca ctaagggact atcgccaact cgtccgggta atttattggt
     5701 cattccgccc gtggacgtgg ctgccgccag attgccgtct aaatccaacg ccacggcccc
     5761 tacggtgccc attttctgtt tttcatccag tggcgcaccg ctatggtcga ggacggttgc
     5821 cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa ggcgtggaga aaatctccgg
     5881 tgagacgcgc tccatgccat gagcaaacgc aaaattttct gctccttcgc caatcatcat
     5941 cacatgcggg ctttgctcca tcaccagccg ggcggcaaga accggattac gcagatgact
     6001 aacgcccgcc accgcaccgg ctttcagggt gttaccatcc atcacacagg cgtccagttc
     6061 atgggtttca tcacgcgtaa agacagcgcc aattccggcg ttaaacagtg gacactcttc
     6121 cagcagacgc accgcttccg tcaccacatc cagcgcactt tcgcccgctt ccagcatttt
     6181 ctgcccggtt tcaacaatgg cagacagcgc ctcgatgtag cgtaattcct gttgcagact
     6241 catctgcgcg cggctaattg cacctgcgcc accatgaatt gcaataactg ctttgcccat
     6301 agtattcgtc cattaagggt ggctcgccac ttttgctata aatatcagaa tttttatgaa
     6361 ttactcattc aatttttgaa tatagaaaga tggatatggc atgtaaaggc aggacccagc
     6421 gctacgcacg aggtggcata cagtctgtgg cgtttttctg acataatagg caaattcgat
     6481 tttgcctccg caggagtgtt ttcatggaat ttaccaccgg attgatgtcg ctcgacaccg
     6541 cgcttaatga gatgctttct cgcgttaccc cactgaccgc ccaggaaacg ctgccactgg
     6601 tacagtgttt tggtcgtatt ctggcgagcg atgtcgtttc gccgcttgat gttccggggt
     6661 ttgataactc cgcaatggac ggctacgcgg tgcgtttagc cgatattgcc tccgggcaac
     6721 cgctgcccgt tgccggtaaa tcctttgccg gtcagccgta ccatggtgaa tggcctgcgg
     6781 gtacctgcat tcgtattatg accggtgcgc cggtgccgga aggctgcgaa gcggtggtga
     6841 tgcaggagca gactaaacaa acggacaatg gcgtgcgttt tactgctgaa gtgcgtagcg
     6901 ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg tgcggttgtt ttcccggcgg
     6961 ggactcgcct gactaccgct gaactgccag tgattgcttc actggggatt gccgaagttc
     7021 cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg tgatgaactc cagttgccag
     7081 gccagccgct gggcgacggc caaatctacg ataccaaccg tctcgccgta cacctgatgt
     7141 tagaacagct gggttgcgag gtaatcaact tagggattat ccgcgacgat ccacatgccc
     7201 tgcgcgccgc atttattgaa gccgacagcc aggcggatgt ggtgatcagt tccggcggtg
     7261 tttcagtggg tgaagcggat tacaccaaaa cgattcttga agagctgggg gagatcgcct
     7321 tctggaagct ggcgattaaa ccaggtaaac cgttcgcgtt cggtaaactc agcaatagct
     7381 ggttctgcgg cctgccgggc aacccggttt cagcgacgct gaccttctat caactggtac
     7441 agcctttgct ggcaaaacta agcggtaaca ccgccagcgg cctgcccgcg cgccagcgcg
     7501 tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct tgatttccag cgcggcgtgc
     7561 tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac cggacatcag ggttcacata
     7621 tatttagctc ctttagtctc ggcaactgct ttatcgtgct ggaacgcgat cgcggcaatg
     7681 tggacgtggg cgaatgggtg gaagtagaac cgtttaacgc gttgttcgga ggcctgtaat
     7741 ggcggaactc agcgatcagg agatgctgcg ctacaaccgg caaatcattc tgcgcggttt
     7801 tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg ctggtagtcg gcctcggtgg
     7861 actcggctgc gcagcttcgc agtatctggc aagcgccggt gtcggcaacc tgacgctgct
     7921 cgacttcgac acggtttcgc tctcgaatct gcaacgccag acactgcaca gtgatgccac
     7981 ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg acgcggatca acccacatat
     8041 cgcgattacg ccagtcaatg cactgctaga tgacgcagaa cttgcagcat tgattgctga
     8101 acacgatctg gtgctcgact gtacggataa cgttgcggta cgtaatcaac tgaacgcagg
     8161 ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca attcgtatgg aagggcaaat
     8221 cacggtcttc acttatcagg acggcgaacc gtgctatcgc tgccttagcc gtttgtttgg
     8281 cgagaacgca ttaacctgcg tagaagcagg cgtgatggca ccgttgatcg gcgtaattgg
     8341 ttcgttgcag gcaatggaag cgatcaaact gctggcaggt tatggcaaac cggccagcgg
     8401 gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc gaaatgaaac tgatgcgcaa
     8461 tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca ggcctgataa gacgcgacaa
     8521 gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg tgaacgcctt atctggccta
     8581 cggttcgggc acagatttcc aagcgcggca ggcatcggtg cactccgcca cgtcatcagc
     8641 aatttatgag gatgcagaac gtccggttaa atcgacgttc tgccaaacgc tccctgccag
     8701 tcctgctcaa acttcgccac agcggcttcc accgccggat agctaatcat ctgttgtgcc
     8761 acatccagtg gcagagtaat tgattcacat cctgccagta agcagtccag cgcctgacgc
     8821 ggggttttga aactcgctgc cagcactttc gcctgcggcg catgcatttt caataactgg
     8881 tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag catcaatacg attaacgtaa
     8941 ggcgcaacat attccgcacc tgccagcgcc gacagcagcc cttgtgctgc gccatatacc
     9001 gcggttccca gcgttggaat cccttccgct tttaacatct taatagctgc cagcccctcg
     9061 gcggtcaccg gaactttcac cacgatatcc gcaataatag aacgcaattt acgtgcgtca
     9121 ttcaccatcc cttcagcagt ggtagccatt acctgggcaa acagacgccc ctgaccgccc
     9181 atcgcttcat gaagttgcgg aagcacaaca tccagcggtt ttttacccgc ggcgataatg
     9241 cttgggttag tggtcacacc cgccagcgga aaaatacgtg acagcgcctt caccgcaaca
     9301 acgtctgaag tatccagata cagttccata accatcctca aaatgtactt tttgtctgaa
     9361 tgtagactat cacgggaaaa ctctctgaac agttgacgca catcaatcta actttcattc
     9421 gaaagtattt taatgcttga acgaaacaga gaggcaacta tgattttcaa tattcagcgc
     9481 tactcgaccc atgatggccc cggtatccgc acggtcgtat ttcttaaagg ctgttcgctg
     9541 ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgca cgcaggatct gctgtatgac
     9601 gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg ccgcgccgga agtgattgag
     9661 cgcgcgctga atggtttgct tattcatcgg gaaaagttaa ccccggagca tctgacggcg
     9721 ttaaccgact gctgtccgac acaggcatta accgtgtgtg gtgaagtgaa aagcgttgag
     9781 gagatcatga cgaccgttct gcgcgataaa ccgttttacg atcgcagcgg cggcggttta
     9841 acgctttcgg gtggtgagcc ctttatgcag ccggaaatgg cgatggcgct actgcaagcc
     9901 agccacgagg caggcattca tactgcggta gaaacctgtc tgcatgtgcc gtggaaatat
     9961 atcgcccctt ctctgcccta tatcgatctg tttcttgccg atttaaaaca cgttgccgac
    10021 gcgccgttta aacagtggac cgacggtaac gccgccagag tgctggataa cctgaaaaaa
    10081 ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc tgattcaggg ctttaatgcc
    10141 gacgaaacct ctgtaaaagc cattaccgat tttgccgccg acgagctgca cgttggcgaa
    10201 attcattttc tgccctacca cacgctgggc atcaacaaat atcacttact taatctgccc
    10261 tatgacgccc cggaaaaacc gcttgatgcg ccagaactgc tcgactttgc ccagcagtat
    10321 gcctgccaga aagggttaac cgcgacctta cgaggataac aaccatgacc acactgaaac
    10381 tggacacgct cagcgaccgc attaaagcgc acaaaaatgc gctggtgcat attgtgaaac
    10441 cgccagtctg taccgagcgc gcgcagcact ataccgagat gtatcaacaa catctcgata
    10501 agccgatccc ggtacgtcgc gcgctggcac tggcgcatca cctggcgaat cgcaccatct
    10561 ggatcaaaca cgatgagttg atcattggca accaggcaag cgaagttcgc gccgcgccga
    10621 tcttcccgga atatactgtc tcgtggatcg aaaaagagat tgatgatctg gcagatcgtc
    10681 ccggtgctgg ctttgcggtg agcgaagaga acaaacgcgt tctgcatgaa gtgtgcccgt
    10741 ggtggcgcgg tcagaccgta caggatcgct gctacggcat gtttaccgat gagcaaaaag
    10801 gtctgctggc gaccggaatc attaaagcgg aaggcaatat gacctccggc gatgcgcacc
    10861 tggcggtgaa tttcccgctg ctgctggaaa aagggcttga tggtctgcgc gagaaagtag
    10921 cggaacgtcg ctcgcgcatc aacctgacgg tgctggaaga tttacacggt gagcaattcc
    10981 tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca cattgaacgt ttcgctgccc
    11041 tggcgcgtga aatggccgcg accgaaaccc gcgaaagccg tcgcgatgaa ctgctggcga
    11101 tggcagaaaa ctgcgatctt atcgcccacc agccgccgca gactttctgg caggcgctgc
    11161 aactgtgtta cttcatccag ttgattttgc agatcgaatc taacggtcac tcagtatcgt
    11221 ttggtcgtat ggaccagtat ctctacccgt actatcgccg cgacgttgaa ctcaaccaga
    11281 cgctggatcg cgaacacgcc atcgagatgc tgcatagctg ctggctgaaa ctgctggaag
    11341 tgaacaagat ccgctccggc tcacactcaa aagcctctgc gggaagtccg ctgtatcaga
    11401 acgtcactat tggcgggcaa aatctggttg atggtcaacc aatggacgcg gtgaatccac
    11461 tctcttacgc gatcctcgaa tcctgcggtc gcctgcgttc cactcagcct aacctcagcg
    11521 tgcgttacca tgcaggaatg agcaacgatt tcctcgacgc ctgcgtacag gtgatccgtt
    11581 gcggcttcgg gatgccggcg ttcaacaacg acgaaatcgt gatcccggaa tttattaaac
    11641 tcggtattga accgcaggac gcttatgact acgcagcgat tggttgtata gaaaccgccg
    11701 tcggtggcaa atggggctat cgctgtaccg gcatgagctt tatcaacttc gcccgcgtga
    11761 tgctggcggc gctggaaggc gggcatgatg ccaccagcgg caaagtgttc ctgccacaag
    11821 aaaaagcgtt gtcggcaggt aacttcaaca acttcgatga agtgatggac gcgtgggata
    11881 cgcaaatccg ttactacacc cgcaaatcaa tcgaaatcga atatgtcgtc gacaccatgc
    11941 tggaagagaa cgtgcacgat attctctgct cggcgctggt ggatgactgt attgagcgag
    12001 cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt ttctggcctg caggtcggca
    12061 ttgccaacct cggcaacagc ctggcggcag tgaagaaact ggtgtttgaa caaggtgcga
    12121 ttggtcagca acagcttgct gccgcactgg cagatgactt cgacggcctg actcacgagc
    12181 agctgcgtca gcggctgatt aacggtgcgc cgaagtacgg caacgacgat gatactgtcg
    12241 atacgctgct ggctcgcgct tatcagacct atatcgacga actgaaacag taccataatc
    12301 cgcgctacgg tcgtggtccg gttggcggca actattacgc gggtacgtca tcaatctccg
    12361 ctaacgtacc gtttggcgcg cagactatgg caacaccgga cgggcgtaaa gcccacaccc
    12421 cgctggcaga aggcgcaagc ccggcctccg gtactgacca tcttggccct actgcggtca
    12481 ttggctcagt gggtaaactg cctacggcag cgattctcgg cggcgtgttg ctcaaccaga
    12541 aactgaatcc ggcaacgctg gagaacgaat ctgacaagca gaaactgatg atcctgctgc
    12601 gtaccttctt tgaagtgcat aaaggctggc atattcagta caacatcgtt tcccgcgaaa
    12661 cgctgctgga tgcgaaaaaa catcccgatc agtatcgcga tctggtagtg cgtgtcgcgg
    12721 gctattccgc gttcttcacc gcgctctctc cagacgctca ggacgatatc atcgcccgta
    12781 ctgaacatat gctgtaatct cctcggcccg gcggcgtcgt cgccgggcta aatcattcac
    12841 atcatcaatt tcatccttac tttcattcga aatataattt gtgctctgcg tcacattgtt
    12901 attacactgt tacggtttca ttttcgagcc aggagcacac ctatgagcgt aaaagttatc
    12961 gtcacagaca tggacggtac ttttcttaac gacgccaaaa cgtacaacca accacgtttt
    13021 atggcgcaat atcaggaact gaaaaagcgc ggcattaagt tcgttgttgc cagcggtaat
    13081 cagtattacc agcttatttc attctttcct gagctaaagg atgagatctc ttttgtcgcg
    13141 gaaaacggcg cactggttta cgaacatggc aagcagttgt tccacggcga actgacccga
    13201 catgaatcgc ggattgttat tggcgagttg ctaaaagata agcaactcaa ttttgtcgcc
    13261 tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg aagcatttgt cgcactgatg
    13321 gcaaaacact accatcgcct gaaacctgta aaagattatc aggagattga cgacgtactg
    13381 ttcaagtttt cgctcaacct gccggatgaa caaatcccgt tagtgatcga caaactgcac
    13441 gtagcgctcg atggcattat gaaacccgtt accagtggtt ttggctttat cgacctgatt
    13501 attcccggtc tacataaagc aaacggtatt tcgcggttac tgaaacgctg ggatctgtca
    13561 ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg cggagatgct gaaaatggcg
    13621 cgttattcct ttgcgatggg caatgctgcg gaaaacatta aacaaatcgc ccgttacgct
    13681 accgatgata ataatcatga aggcgcgctg aatgtgattc aggcggtgct ggataacaca
    13741 tcccctttta acagctgacc tctctaccgg agccgtgcgc tccggttttc ccttcttttt
    13801 acatcctgta catcacatca aatcattaaa cttgcattaa cttatttata acttatattg
    13861 tcacatgcaa atcagaatgg tttcgaatga aagttaatga gaggtggtta tggcttctac
    13921 ttttaccagc gacacattgc ctgccgatca caaagcagct atccgtcaga tgaagcacgc
    13981 gctgcgggcg cagcttggcg acgtccagca gatctttaat cagctaagcg atgacattgc
    14041 cacgcgagtg gctgaaatca acgcactcaa agcacagggc gatgccgtct ggccggtgct
    14101 gtcttatgcc gatatcaaag caggtcatgt tactgcagag cagcgcgaac agattaaacg
    14161 tcgcggttgt gcggtgataa aaggccattt cccccgcgaa caagcgctag gctgggatca
    14221 gtcgatgctg gactatctgg accgcaaccg ctttgacgag gtctacaaag gccccggcga
    14281 taatttcttc gggacgctca gcgcttcacg tcccgagatt taccccatct actggtcgca
    14341 ggcgcaaatg caggcccgcc agagtgaaga aatggcgaat gcgcagtcgt ttctcaatcg
    14401 tctgtggaca tttgaaagtg atggaaagca atggtttaac ccggatgtga gcgtcatcta
    14461 ccctgaccgt atccgccgcc gtccgcccgg aacgacctcc aaaggtcttg gagcgcatac
    14521 cgactccggg gcactggaac gctggctgct tccagcgtat cagcgcgttt tcgccaacgt
    14581 ctttaatggc aatctggcgc aatatgatcc ctggcatgcg gcacatcgta cggaagttga
    14641 agagtacacg gtggacaaca ccaccaaatg ttccgtgttt cggacattcc agggctggac
    14701 agcgctctct gatatgctgc ctggtcaggg gctgctgcac gtcgtgccca ttcctgaagc
    14761 tatggcgtac gtactgttac gtccgctgct tgatgatgtg ccggaggatg aactgtgcgg
    14821 cgtagcgccc ggaagagtat tgccggtatc agagcaatgg catccactgt tgattgaggc
    14881 gttaaccagc attccaaaac tcgaagccgg agactccgtc tggtggcact gcgacgtcat
    14941 ccattccgtt gcccccgttg aaaatcaaca gggttggggc aacgtgatgt acattcctgc
    15001 ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag gtgaaggccg cactggaaaa
    15061 aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa acaaactggg aaggacgctt
    15121 tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg ggcatggatg tttgagaatt
    15181 aatggaagtg aattgatgtg ggattaagaa aatcgattcg acgttactca gattgctgac
    15241 aaatgcgctt tgttaatgcc tgatgcggcg ttaacgcctt atccggccta caaagtcctg
    15301 cagattcaat atattgcagg tactgcgtag gcctgataag cgtagcgcat caggctattt
    15361 tgcatttgtc atcagccgcc gtcaccccga cagtatcggg gtgacggata atcacttact
    15421 cgatcccttt actacgcagg taatcttcgt aattaccgct aaagtcgatc acgcgttccg
    15481 gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa ctcacggtcg tgtgaaacaa
    15541 agatcagcgt gccctgatac agttccagtg ccatgttcag cgactcaatg gattccatat
    15601 ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg cttctgcatc attaacttac
    15661 caaacagcat ccgccctttt tccccaccgg aaagcacttt agctggcttt ttgatgtcgt
    15721 cctggctgaa cagcaaacga ccgagaatac tgcgtaccgc ctgctcgtca tcgccttcct
    15781 gcttccactg gctcatccat tcgaacacgg tcagatcatt ttcaaactca tattcgtggt
    15841 cctgagcata gtaaccaatg cgcgcgttct cagaccattt tacggtgccg ctgtccggtt
    15901 gcagatcgcc caccagcgtt ttcagcagcg ttgatttacc gacgccgttg gtacccagta
    15961 ccgccagttt ttcacccact tccagcagca ggttgagatt tttaaacagc ggaccgttat
    16021 caaacccttt ggtcagacct tccacttcca gcgcgttacg gaacagtttc ttatcctgtt
    16081 caaaacggat gaacgggttc tgacggctgg aggctttcac ctcttccagt ttgattttat
    16141 caatctggcg cgcgcgcgaa gttgcctggc gagatttcga ggcgttggcg ctaaagcggc
    16201 taacgaaaga ttgcaactca gcaatctgcg ctttcttctt ggcgttatcg gccagcagac
    16261 gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta gttacccgga taaacgcgca
    16321 gctcgccgta atccagatcc gccatgtggg tacagaccat gttaaggaag tgacggtcgt
    16381 gcgagatgat gatcatggtg ctgtcacgct cgttcagcac ctgttccagc cagcgaatgg
    16441 tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag aatatccgga tcagcaaaca
    16501 gcgcctgcgc cagaagcaca cgcagcttcc agccaggagc aacttcactc atcgggccgt
    16561 agtgctgttc cactggaatt cccacgccaa gcaacagttc accggcgcga gcttccgcag
    16621 agtaaccgtc catttcgccg tatttaactt ccagatcggc cactttatag ccgtcttcct
    16681 cactcatttc cggcaaagca tagatgcggt cgcgctcctg cttcacttcc cacaactctt
    16741 tatgccccat gatcaccgta tccagcacag tgaactcttc aaaggcaaac tgatcctgac
    16801 gcagtttacc aatgcgctcg ttgggatcga gggaaacgtt acccagcgtc ggctcaaggt
    16861 cgccgccgag gatcttcata aaggtggatt taccactacc gttcgcgcca atcaggccgt
    16921 aacggttgcc gccgccaaat ttgacggaaa tgttttcaaa caacggctta ctgccgaact
    16981 gcatggtgac gttactggaa actaacacag gcgtatcctg aaaagagata tgacaaaccg
    17041 cgcattatgc cataagtctg aaagaagatc ctgctcctcg cttaaggtct acactcaagc
    17101 caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac tgtgagctgg aatgaactta
    17161 taatgcgctt ccaatactct aatattctca acccaatggc ctgccaggca caaaatctcg
    17221 cttaacatga atatgaaatt gaaaacatta ttcgcagcgg ccttcgctgt tgtcggcttt
    17281 tgcagtaccg cctctgcggt aacttatcct ctgccaaccg acgggagtcg cctggttggt
    17341 cagaatcagg tgatcaccat tcctgaaggt aacactcagc cgctggagta ttttgccgcc
    17401 gagtaccaga tggggctttc caatatgatg gaagcgaacc cgggtgtgga taccttcctg
    17461 ccgaaaggcg gtactgtact gaacattccg cagcagctga tcctgccgga taccgttcat
    17521 gaaggcatcg tcattaacag tgctgagatg cgtctttatt actatccgaa agggaccaac
    17581 accgttatcg tgctgccgat cggcattggt cagttaggca aagatacgcc tatcaactgg
    17641 accaccaaag ttgagcgtaa aaaagcaggc ccgacctgga cgccgaccgc caaaatgcac
    17701 gcagagtacc gcgctgcggg cgaaccgctt ccggctgtcg ttccggcagg tccggataac
    17761 ccgatggggc tgtatgcact ctatatcggt cgcctgtatg ctatccatgg caccaacgcc
    17821 aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc gtctgcgtaa cgaagacatc
    17881 aaattcctgt tcgagaaagt accggtcggt acccgcgtac agtttattga tgagccggta
    17941 aaagcgacca ccgagccaga cggcagccgt tatattgaag tccataaccc gctgtctacc
    18001 accgaagccc agtttgaagg tcaggaaatt gtgccaatta ccctgacgaa gagcgtgcag
    18061 acagtgaccg gtcagccaga tgttgaccag gttgttcttg atgaagcgat taaaaaccgc
    18121 tccgggatgc cggttcgtct gaattaatct tcaaaactta aagcaaaagg cggactcata
    18181 atccgccttt tttatttgcc agaccctagt tggccgggag tataactaac aaaacatatc
    18241 ccaccaacgc cgcccacaac agcatcggta ttgaatagag atggaaacgc caccagatgc
    18301 ggcgatcgtt cgccatacgt agcgcaatca aatttgccag cgatccgggt aataacccaa
    18361 agccacctac gtttaccgcc cataccagta ataaagacgg cggcacatag ttcagcaaca
    18421 atatggtact cggcacgtta ctgatcacct gcgataaacc gattgccgtt aaccataacc
    18481 cgggttcaga tagatgactc acgttaccca acacgccttg caacgctgga agctgggtca
    18541 gtaaatggac gtcgataaac atcgccataa acaccagcag cagcgtccag tccacactga
    18601 gcaccacgcg acgtgccagc agcgcaaagc ctgccgccac aatcaccagt ccccacagct
    18661 cttgtttgaa ctccagcgcc gtcagaaaga cgatatacag ccccagacaa ctccacacca
    18721 gccgcggttt ccactccggt gtttgcaccc ccgtatggta ttgcagcgcc tttccaggga
    18781 aacaacacca gcacaggagc aggagcgtca gcatcattgc gccagccagc ggtgccattt
    18841 gggcaataaa tccggcaaac gaaagaccag aacgtcccca gataagaata ttttgcgggt
    18901 tgccaattgg cgtcagtagc gaaccagcgt tgactgccag cgcctcaaaa ataatcagcc
    18961 gattaaccgg gatctcacac agtcttttta gcgtgatagt cagcggaaca acaataaaca
    19021 gcgcgacatc gttggtcaga aaggtagaaa gcagcgccgc cgccagcacc ataaacatcg
    19081 ccagccgacg ctccgtagca aagcggcgca ccattttgcg ccccagcaca tcaaaataac
    19141 cgcttaactc cacacctttg gtcagcagca tcaggccgct taaggtgatg atggtgtgcc
    19201 agtcgatagc agcaggccag gatttcggtg caaagggcac gaaaaagctt aatccgatac
    19261 caacaagaat taataactga aaaaaacgat cgccttgcag cgtgcgtaaa aaaggcaggc
    19321 tcatttggca ccgtgtttct gggtaaacaa acgaaaagcg tcgagcgtct cttcactaac
    19381 atggtgctcc atgccttccg cgtcgcgacg ggcgatttcc ggactgacgc ccaacaccaa
    19441 caagaaattt tcgactatct gatgtcgctc gcggctttcc tgcgccagct tctctccttc
    19501 tgccgttaaa aacacgccac gccaggggat catttcaatc agccccatgg ttgccagccg
    19561 cttaagcatt ttagccaccg tcggttgcga aactcccaga cgagcagcca tgtccacctg
    19621 acgagcttcc cccacttccc tgatcaagtc agaaatcagc tcaacgtaat catcaataag
    19681 ctcgcgccga tgcgcctctc tgacctggcg gaacccttca acgtgctctt ccacgttcac
    19741 taactgcgtc actttttttg ctgttggcgt acctgcgcga cgactcattg tgcttcctca
    19801 ttatggtgac gcatacatag cgttctcatt tcgtgcgcac attgtaaacc agagttgcga
    19861 aggtacaaaa aattaacgtt ttagcaatag ctatataata tagcctgtgc tatatctgta
    19921 tgtaatgcaa tcatccctca aggatcgacg ggattagcaa gtcaggaggt cttatgaatg
    19981 agttcaagag gtgtatgcgc gtgtttagtc attctccctt taaagtacgg ttaatgctgc
    20041 tctctatgtt gtgcgatatg gtcaacaaca aaccgcagca agataaacct tccgataaat
    20101 agcggcgtcg cggtacgccg cttcactcct gctttcatgc aggcataacg cgttttggtc
    20161 tgaaaaaccc cactttttgt cggatttgca atccccttcg caaaagattt gttcgtcagt
    20221 agttgacctg aacggcggct cgctctatct tcttgcagcc ctgcgtatat tgcggctcgc
    20281 ggatgcggac ccctttcccc tcttcacgca ctcttgcagg tattgaccct tgacgccagg
    20341 gtaagcacat ggcgtttgtt acgatagtgg catatgaatt taaccctcaa agaatcgctt
    20401 gttacccgta gccgggtatt tagcccgtgg actgcgttct actttttaca gtcgctatta
    20461 attaacctcg gcttaggtta ccccttcagt ttgctctaca ccgctgcgtt tacggctatt
    20521 ttgcttttgc tatggcgaac attgcctcgc gtacaaaaag ttctggtcgg tgtcagttcg
    20581 ctggtggcgg cttgttattt cccttttgct caggcctacg gcgcgcctaa tttcaataca
    20641 ttgctggcat tgcactccac caatatggaa gagtcgaccg aaatcctgac gatttttccg
    20701 tggtacagct acctggtcgg cttatttatt tttgcgctcg gcgtaatagc aatcaggcga
    20761 aaaaaagaga atgaaaaagc gcgctggaat accttcgaca gcctgtgtct ggtattcagt
    20821 gtggcgacat tttttgttgc tcccgtgcaa aacctggcct ggggtggcgt atttaaactg
    20881 aaagatactg gctatccggt atttcgtttt gctaaggatg tcatcgtcaa taataacgag
    20941 gtgattgaag agcaagaacg gatggcaaaa ctttccggaa tgaaagatac ctggacggtc
    21001 actgccgtta agccgaagta tcagacctat gtggtggtga tcggtgaaag cgcgcgtcgc
    21061 gatgccctcg gtgcctttgg cggtcactgg gacaataccc cgtttgccag cagcgttaac
    21121 ggtttgatat ttgctgacta cattgccgcc agtggctcca cgcagaaatc gcttggctta
    21181 acgctcaatc gcgtagtcga tggcaaacca cagtttcagg ataactttgt caccctggca
    21241 aatcgcgcgg gcttccagac ctggtggttt tccaaccagg gccaaatcgg cgaatacgat
    21301 actgctatcg ccagcatcgc caaacgtgca gatgaagtgt acttcctgaa agaaggtaat
    21361 tttgaagcag ataaaaacac gaaagacgaa gcgttactgg atatgaccgc tcaagtgctg
    21421 gcgcaagagc actcgcaacc gcagctgatt gttctgcatc tgatgggctc gcatccgcag
    21481 gcctgcgaca ggacacaagg caaatacgaa acctttgtgc aatcgaaaga aacgtcgtgc
    21541 tatctctata ccatgacgca aacggacgat ttactgcgca agctgtacga tcagttacgc
    21601 aacagcggca gcagcttctc tctggtttac ttttctgacc acggtctggc ctttaaagag
    21661 cgcggcaaag acgtgcaata ccttgcccat gatgataaat atcagcaaaa tttccaggtg
    21721 ccttttatgg tcatttccag cgacgataaa gcgcatcggg tgattaaagc ccgccgctca
    21781 gccaatgact tcttaggctt tttctcgcag tggacgggaa ttaaagcgaa ggaaataaat
    21841 atcaaatacc cgtttatatc tgagaagaaa gccgggccga tatacatcac caacttccag
    21901 ttacagaagg tggattacaa ccatctcgga accgatattt tcgacccgaa accttaaaac
    21961 aaaaaatccg ccccgagagg cggatttttt atatcaccaa agtgattaga agcggtaacc
    22021 aacaccggca atccaggtgc ctacgtcaac gctacgaata cggctctgct cgtaagagaa
    22081 gtccagagca acgttttcca tcgggttgaa ctgcagacct gcaccgtagg agaaaccgta
    22141 gtcgctggtg tcgtgtttgt aggtcgggta ttcagtggtc tggaatttac cataacccac
    22201 ccccactaca ccgtagatgc ttgcccagtc gttaatgcgg taagccggac cagcagtgat
    22261 gccgtagtac tggtttttgt tgtagtcacc agagcttgca gtacggcttt tctcggtgta
    22321 agtgaaagaa ccgatcacac ccagcgggct gttgtcttct tcatagcggt atttcaggtt
    22381 gaaaccgccc attttgttca tttggccctg agcgtcgctc tgtgcgtaac cgccagttac
    22441 ggtagaagtc gcagctacgg aagtacctgc ggtgaaagcc agaactgcgg ccagtgctga
    22501 aagacatgca atttttttca taaccacctc aaatgtgatt caaataagtc ctaagtttta
    22561 aatatatcaa aaattaatgg gaaactcttc gcgatttgtg atgtctaacg ggccatttca
    22621 tgtaacagaa cgtttccata caccgctatc catctaaatt taaatcactt tttcagagaa
    22681 ctgcgtaagt attacgcatg ttttccctgt cattcatcca gattattcct aatcaccaga
    22741 ctaatgattc catcaatcct ggcgcatttt agtcaaaacg ggggaaaatt ttttcaacaa
    22801 atgctcaacc agcattgggt atatccagta cactccacgc tttacttaag tctagatatt
    22861 tgtgggagaa aggatgcctg gttcattacg taaaatgccg gtctggttac caatagtcat
    22921 attgctcgtt gccatggcgt ctattcaggg tggagcctcg ttagctaagt cactttttcc
    22981 tctggtgggc gcaccgggtg tcactgcgct gcgtctggca ttaggaacgc tgatcctcat
    23041 cgcgttcttt aagccatggc gactgcgctt tgccaaagag caacggttac cgctgttgtt
    23101 ttacggcgtt tcgctgggtg ggatgaatta tcttttttat ctttctattc agacagtacc
    23161 gctgggtatt gcggtggcgc tggagttcac cggaccactg gcggtggcgc tgttctcttc
    23221 tcgtcgcccg gtagatttcg tctgggttgt gctggcggtt cttggtctgt ggttcctgct
    23281 accgctgggg caagacgttt cccatgtcga tttaaccggc tgtgcgctgg cactgggggc
    23341 cggggcttgt tgggctattt acattttaag tgggcaacgc gcaggagcgg aacatggccc
    23401 tgcgacggtg gcaattggtt cgttgattgc agcgttaatt ttcgtgccaa ttggagcgct
    23461 tcaggctggt gaagcactct ggcactggtc ggttattcca ttgggtctgg ctgtcgctat
    23521 tctctcgacc gctctgcctt attcgctgga aatgattgcc ctcacccgtt tgccaacacg
    23581 gacatttggt acgctgatga gcatggaacc ggcgctggct gccgtttccg ggatgatttt
    23641 cctcggagaa acactgacac ccatacagct gctggcgctc ggcgctatca tcgccgcttc
    23701 aatggggtct acgctgacag tacgcaaaga gagcaaaata aaagaattag acattaatt
//
LOCUS       DAOFQD010000069        23383 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.069, whole genome shotgun sequence.
ACCESSION   DAOFQD010000069 DAOFQD010000000
VERSION     DAOFQD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.069"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..193
                     /locus_tag="QBF46_002821"
     CDS             <1..193
                     /locus_tag="QBF46_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDQ6674982.1"
                     /translation="WQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKY
                     LSDHPKLQGIAQQNSFKHT"
     gene            349..1884
                     /gene="gadC"
                     /locus_tag="QBF46_002822"
     CDS             349..1884
                     /gene="gadC"
                     /locus_tag="QBF46_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDQ6674983.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2111..3430
                     /locus_tag="QBF46_002823"
     CDS             2111..3430
                     /locus_tag="QBF46_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDQ6674984.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3802..6573
                     /gene="dosP"
                     /locus_tag="QBF46_002824"
                     /gene_synonym="pdeO"
     CDS             3802..6573
                     /gene="dosP"
                     /locus_tag="QBF46_002824"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDQ6674985.1"
                     /translation="MKMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQVQNAQSWSATIRQR
                     DGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGL
                     PKRNNLHNYLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLK
                     PDQYLCRIEGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYD
                     VGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLK
                     LVYQPQIFAETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEAC
                     RQLAEWRSQNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMM
                     EHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKR
                     ILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGW
                     MSSVLPLKI"
     gene            6831..7412
                     /gene="ddpX"
                     /locus_tag="QBF46_002825"
     CDS             6831..7412
                     /gene="ddpX"
                     /locus_tag="QBF46_002825"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDQ6674986.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            7426..8976
                     /locus_tag="QBF46_002826"
     CDS             7426..8976
                     /locus_tag="QBF46_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ6674987.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASGWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLILVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            8978..10000
                     /locus_tag="QBF46_002827"
     CDS             8978..10000
                     /locus_tag="QBF46_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6674988.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDHIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            9997..10893
                     /locus_tag="QBF46_002828"
     CDS             9997..10893
                     /locus_tag="QBF46_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDQ6674989.1"
                     /translation="MMLSEDTSAVRPAKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGTAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            10890..11876
                     /locus_tag="QBF46_002829"
     CDS             10890..11876
                     /locus_tag="QBF46_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6674990.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            11869..12795
                     /locus_tag="QBF46_002830"
     CDS             11869..12795
                     /locus_tag="QBF46_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6674991.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(12851..13282)
                     /gene="osmC"
                     /locus_tag="QBF46_002831"
     CDS             complement(12851..13282)
                     /gene="osmC"
                     /locus_tag="QBF46_002831"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDQ6674992.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            13627..13842
                     /gene="bdm"
                     /locus_tag="QBF46_002832"
     CDS             13627..13842
                     /gene="bdm"
                     /locus_tag="QBF46_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDQ6674993.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            13944..14081
                     /gene="sra"
                     /locus_tag="QBF46_002833"
     CDS             13944..14081
                     /gene="sra"
                     /locus_tag="QBF46_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDQ6674994.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            14238..15935
                     /gene="maeA"
                     /locus_tag="QBF46_002834"
     CDS             14238..15935
                     /gene="maeA"
                     /locus_tag="QBF46_002834"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDQ6674995.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGMV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            16069..17079
                     /gene="adhP"
                     /locus_tag="QBF46_002835"
     CDS             16069..17079
                     /gene="adhP"
                     /locus_tag="QBF46_002835"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDQ6674996.1"
                     /translation="MKAAVVTKDHHVDVTYKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGITSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSHTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            17225..17509
                     /locus_tag="QBF46_002836"
     CDS             17225..17509
                     /locus_tag="QBF46_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDQ6674997.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(17738..18391)
                     /gene="fdnI"
                     /locus_tag="QBF46_002837"
     CDS             complement(17738..18391)
                     /gene="fdnI"
                     /locus_tag="QBF46_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDQ6674998.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(18384..19268)
                     /gene="fdnH"
                     /locus_tag="QBF46_002838"
     CDS             complement(18384..19268)
                     /gene="fdnH"
                     /locus_tag="QBF46_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDQ6674999.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLMACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(19281..22328)
                     /gene="fdnG"
                     /locus_tag="QBF46_002839"
     CDS             complement(19281..22328)
                     /gene="fdnG"
                     /locus_tag="QBF46_002839"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(21741..21743),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDQ6675000.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            22560..>23383
                     /gene="yddG"
                     /locus_tag="QBF46_002840"
     CDS             22560..>23383
                     /gene="yddG"
                     /locus_tag="QBF46_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDQ6675001.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQG"
BASE COUNT         5692 a         5964 c         5916 g         5811 t
ORIGIN      
        1 ctggcaggtt ccggccttca ctctcggcgg tgaagccacc gacattgtgg tgatgcgcat
       61 tatgtgtcgt cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc
      121 ctccctgaaa tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt
      181 taaacatacc tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca
      241 atttccaaag tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag
      301 aacaaaacag gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca
      361 gtacagacag gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg
      421 atggtaatgg ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc
      481 ttcctgctat taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg
      541 gccaccgtcg acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg
      601 ccgagatggg gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt
      661 ccgatgctct atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctgaat
      721 gaagacccca ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg
      781 cagtttggtg gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc
      841 ctgttacctg catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc
      901 gttgctatcg aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg
      961 gttgtatttg ttgccttcat tttgagttat atgggcgtag aagcatccgc aacccacgtc
     1021 aatgaaatga gcaacccagg gcgcgactat ccgttggcta tgttactgct gatggtggcg
     1081 gcaatctgct taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa
     1141 atcaacctct ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg
     1201 gaaattgagt ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa
     1261 atcgcctcct ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg
     1321 ctgccagcgg cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg
     1381 cagctggtga ttacgtctat cgcgttgatt atcctcacca atactggtgg tggtaacaac
     1441 atgtccttcc tgatcgcgct ggcgctgacg gtggtgattt atctgtgtgc ctatttcatg
     1501 ctgtttattg gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat
     1561 atccctggtg gtaaaggggt gaaactggtc gtggctattg tcggtctgct gacttcaatt
     1621 atggcgttta ttgtttcctt cctgccgccg gataacatcc agggtgactc taccgatatg
     1681 tatgttgaat tactggtcgt tagtttcctg gtggtacttg ccctgccctt tattctctat
     1741 gctgttcatg atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt
     1801 cagaacgcac caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt
     1861 gtgatgaatg acaagaaaca ctaaccatgt aacagtcaga acgcaccaaa aggtcacttc
     1921 ttcctgcacc cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa
     1981 ccatgtctga atggttataa gacaagggag cgataattca tcgctccctt tttcgtgctt
     2041 gctgtcgtat tgacttaacc gggataagta cgagaatgag cgcacatctg tttaccggaa
     2101 accagcacat atggatatct gctcccgaaa cgagaaatta gcgattagaa gaccagcgat
     2161 actggttgca ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt
     2221 gacaccacca gcgggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg
     2281 tggcatctgg ctggccacgg tgtctcgcct cgactggcca ccggtttcct cggttaacat
     2341 tagtaacccc accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca
     2401 tctgcaacgt ctcggtataa acacggtctt tttccaggtt aagccggacg gtaccgccct
     2461 gtggccatcg aaaattttgc catggtccga tcttatgacc ggtaagattg gtgaaaatcc
     2521 gggttacgat ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca
     2581 cgcctggttt aacccctatc gcgtatcggt taatacgaag cccggtacta tcagggaact
     2641 gaatagcact ctgtctcaac aaccggcgag cgtctatgtg caacaccgtg actggatcag
     2701 aacgtctggc gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac
     2761 atcaatagtc gcggaagtgg tttcccgcta tccggtagat ggcgtgcagt ttgacgacta
     2821 tttctatact gaatcaccgg gttcacggct aaatgataac gaaacgtacc gcaaatacgg
     2881 aggcgcattt gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa
     2941 agtatcgcac accattaaaa gcattaagcc gggagtcgaa tttggcgtta gcccggcagg
     3001 cgtgtggcgt aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta
     3061 tgacgaatcc tacgctgaca ctcgtcgatg ggttgaacaa ggattgctgg attacattgc
     3121 tccccaaatt tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg
     3181 gtgggcggat gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa
     3241 agtgggtgaa ccttcaaaga tagagccaga ctggatgatt aacggcggcg taccggagct
     3301 gaaaaagcag ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg
     3361 tgaggactat ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg
     3421 gggcagttaa actcccattc ttactttgta acaagccggt gctttaccca ccggcttgtt
     3481 accctcttga actatcccac tagtaatcat gcttactaaa gtcaaattaa ccacgcaatt
     3541 aaccacactt agttaaacag ccataatccc cctaataaaa atgtatgaat atgaaaaacc
     3601 atacgatatc cgcagtcacc gaaaaataat tacgatgggc tatactcttc tgaagaacag
     3661 tagcgtagat tgaaactgtc ctaagtcgct tatttctttt cagtatatct tcatatttca
     3721 ggagaatatg gtgcagttag tcgatctcgc acgttgcgtt ttgattccgt ctgatttccc
     3781 agccttataa gggtgtgaat tatgaagatg tattttaaaa gaatgaaaga tgagtggacc
     3841 ggacttgtcg aacaagcaga tccactcatt cgtgctaaag ccgcggaaat tgccgttgcg
     3901 catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc gcatgccgaa
     3961 gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga acgctggatt
     4021 attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat ccagcatacc
     4081 gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat ggggtttcgg
     4141 gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattctgc cgcagaaaaa
     4201 cttcaggtct atcatttctc gattaacagt attgatatcg cgatggaagt gatgacccgc
     4261 gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg tatcttctcg
     4321 ttgctggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact ttcatgggaa
     4381 atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt gcctttaagc
     4441 caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag cggcattgcc
     4501 gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa tcaaaccatg
     4561 cgtaacacca ggaatttgaa taacagaagt ctgcgggtga aatttttatt acagataaga
     4621 aataccgtat cgcaaattat taccttgctg cgtgaattgt ttgaagaagt atcgcgccac
     4681 gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc gactatcttc
     4741 aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct gattattgac
     4801 gttgataaat tcaaagagat caacgatacg tggggccata acactggcga tgaaattctg
     4861 cgtaaagtct ctcaggcctt ttatgacaac gtccgcagta gtgattatgt tttccgctac
     4921 gggggcgatg aatttatcat tgtattgact gaagcttctg aaaacgaaac gttacgtacc
     4981 gcagaacgta ttcgcagtcg ggtggagaaa accaaactga aagccgcaaa cggcgaggat
     5041 attgccctct cactttccat cggtgccgcc atgtttaatg gtcatcctga ctatgagcgc
     5101 ctcattcaag ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg tgatggcgcg
     5161 cctgcgggga tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc ctttatcgaa
     5221 cgcgtggcag atatcagcca gcatatggcc gcgctggcgc tggaacagga aaaaagccgt
     5281 cagcatattg aacaactcat ccaatttgat ccgatgaccg gtctgccaaa acgcaataac
     5341 ctgcacaatt acctcgatga ccttgtcgac aaagccgtct ctccggtggt gtatctcatc
     5401 ggtgttgacc atattcagga tgtgattgat agccttggct atgcgtgggc cgatcaggca
     5461 ttgctggaag tggtcaatcg ctttcgtgaa aaactcaaac cggatcagta tctctgtcgt
     5521 atcgaaggta cgcagtttgt cctcgtgagc ctcgaaaacg acgtcagtaa cattacccaa
     5581 atcgccgatg agctacggaa tgtggtcagc aagccgataa tgattgacga taaacccttc
     5641 ccgcttacct tgagtattgg catcagctac gacgtgggta aaaaccgcga ttacttgctc
     5701 tccactgctc acaatgcaat ggattatatt cgcaagaatg gcggtaacgg ctggcagttc
     5761 ttcagcccgg cgatgaacga aatggtaaaa gagcgtttgg ttttaggcgc agcgctgaaa
     5821 gaagcgatta gcaataacca actgaaactg gtttaccagc cgcaaatctt cgcagaaacg
     5881 ggtgaactgt acggcatcga agcccttgct cgctggcacg atccccagca tggtcatgtg
     5941 cccccttcac ggtttattcc tctggcagaa gagattggtg aaatcgaaaa tattgggcgc
     6001 tgggtcatcg cggaagcttg ccgtcagtta gcagaatggc gtagccagaa tattcatatc
     6061 ccggcgcttt ccgttaactt gtcggcactg cactttcgca gtaatcagct gcctaatcag
     6121 gtgtctgatg caatgcacgc ctggggtatt gacggccacc agctgacggt agaaatcacg
     6181 gaaagcatga tgatggaaca cgataccgaa atctttaagc gcattcagat cctgcgtgat
     6241 atgggcgtgg gcttatcggt agatgatttt ggtacgggct tttccggatt atcccgctta
     6301 gtcagtcttc cggtaacgga aatcaaaatt gacaaaagtt ttgtcgatcg ttgtctgacc
     6361 gaaaaacgca tccttgcctt acttgaagcc attaccagca ttgggcaaag cctcaattta
     6421 accgtcgtgg cggaaggcgt cgaaaccaaa gagcaatttg agatgctacg caagatccac
     6481 tgtcgcgtta ttcagggata tttcttttcc cgccccctac ccgccgaaga aattccaggc
     6541 tggatgagca gcgtgttacc gctgaaaatc tgacaaattc ctctcgcccg cactcgcggg
     6601 ttttcattta acgtgacact gtcacttaaa tactgtgatt tcagctacga ttccgggaga
     6661 ttccttcctt aacttcccct cttgcaccaa aactgcacta cgctgcacaa atgttgatca
     6721 aaagttaaaa aatataactt taagccagca ttctggcgct tatcccggca tggcatgaga
     6781 tctgcataag cggaaagcgc agcaattttt gtcttataca ggaaccgttt atgtcggata
     6841 ccaccgaact ggttgattta gccgtaatct tccctgatct ggagatcgaa ttgaaatacg
     6901 cctgcgctga taacatcaca ggtaaagcta tttatcagca agcgcgttgt ctgttacaca
     6961 aggatgcgat taccgcgctg gcgaaaagta tcagtatcgc ccagctgtca ggcttacaac
     7021 tggtgattta cgatgcgtat cgcccacaac aagcacaggc gatgttgtgg caagcctgcc
     7081 cagacccgca atatgttgtt gatgtgacgg tcggttctaa tcacagccgt ggcacggcga
     7141 tcgacctgac gcttcgtgat gagcacggga acatcctcga tatgggcgca gggttcgatg
     7201 aaatgcacga gcgctcccat gcttaccatc cttccgtccc gcccgccgct cagcgcaatc
     7261 ggctgttgct gaatgcgatt atgactggtg gcggttttgt cggtatctcc agcgaatggt
     7321 ggcacttcga attaccccag gcagcgagtt accctctgct cgctgatcaa ttcacctgtt
     7381 ttatatcccc tggcacacag cacgtcagtt aatcaggaaa ctgtcatgaa gagatcgata
     7441 tcgtttcgtc ccacattgct cgcgctcgtc cttgccacaa ctttcccggt tgcgcacgcc
     7501 gccgtaccga aagatatgct ggtgattggt aaggccgccg atccacaaac cctcgacccg
     7561 gcggtaacaa tagataataa cgactggaca gtgacctacc cgtcttatca gcggctggtt
     7621 cagtacaaaa cggacggtga taaaggctca accgacgttg aaggcgatct ggcgagtggc
     7681 tggaaagcgt ctgacgatca aaaagagtgg acgttcaccc tgaaagataa tgctaaattt
     7741 gccgatggca cacctgtcac tgccgaagca gtaaaacttt cttttgagcg gctactaaaa
     7801 atcggccagg ggccagcaga agcatttccc aaagatttaa agattgatgc tcccgacgaa
     7861 catacggtga agtttaccct tagccagcca ttcgcaccgt tcctctacac gctggcgaat
     7921 gacggtgcat ccattatcaa tcctgcggtg ttaaaggaac atgcggcgga tgatgcccgt
     7981 ggtttcctcg cgcaaaatac cgctggctcc ggaccgttta tgctgaaaag ctggcaaaaa
     8041 ggtcagcaat taattctggt gccaaatccg cattaccccg gcaataaacc gaactttaag
     8101 cgagtatcgg taaaaattat cggtgaaagt gcctcccgtc gcctgcagct ctcccgtggc
     8161 gacattgaca ttgccgatgc gctgccggtg gatcaactca acgccctgaa gcaggaaaat
     8221 aaagtcaatg tggcagagta tccgtcactg cgcgtcacct atctgtatct gaataacagc
     8281 aaagcgccac ttaatcaggc ggatctgcgt cgggccattt cctggtctac cgattatcag
     8341 ggcatggtta acggcattct gagtggtaac ggaaaacaga tgcgcggccc gattccggaa
     8401 ggcatgtggg gctacgatgc gacggcaatg caatacaacc atgacgaaac gaaagccaaa
     8461 gccgaatggg ataaagtgac gagcaaaccc accagcctga cgtttctcta ctccgataac
     8521 gatccgaact gggagcctat tgctttggcg acacaatcca gtctcaacaa gctgggcatc
     8581 aatgtgaagc tggaaaagct ggcgaacgcc accatgcgcg acagagtggg taaaggtgat
     8641 tacgacattg cgattggcaa ctggagtccg gattttgccg acccgtatat gtttatgaat
     8701 tactggtttg agtcagacaa aaaaggtctg ccgggtaacc gctcgttcta tgaaaacagt
     8761 gaggtcgata agttactgcg caatgcgctt gcgaccaccg accagacgca gcgtacccgg
     8821 gactaccagc aggcacagaa aatcgtcatt gatgacgctg cttatgtgta cctgttccag
     8881 aaaaactacc aactggcgat gaacaaagag gtgaaaggct ttgtgttcaa tcccatgctg
     8941 gaacaggtct tcaatatcaa taccatgagt aaataacgtg accttctgga gtattttacg
     9001 ccaacgctgc tgggggctgg tgctcgtggt ggcgggcgtc tgcgtgatta cgtttattat
     9061 ctcgcatctg atccctggcg atccggcgcg gttactggcg ggtgaccgcg ccagcgatgc
     9121 tatcgtggaa aatattcgcc agcaactggg actggaccag ccactgtatg tacagtttta
     9181 ccgctacgtc agcgacctgt ttcatggtga cctgggaaca tccattcgta ccgggcgtcc
     9241 ggtgctggaa gagttgcgta tatttttccc ggcgacgctg gaactggctt tttgtgccct
     9301 gctgctggca ctcctgattg gcatcccgct gggcatactc tctgctgtct ggcgaaatcg
     9361 ctggctggat catctggtgc gaataatggc cattaccgga atctccacac ctgcgttctg
     9421 gcttgggctg ggcgtcattg tgctgtttta tggtcatctg caaattcttc ccggcggcgg
     9481 aaggcttgat gactggctgg atccaccaac gcacgttacc ggcttttatc tgctcgatgc
     9541 gctgcttgaa ggtaacggtg aagtcttctt caatgcgctg caacatctca tcttaccggc
     9601 attaacgctg gcgttcgttc acctggggat tgtcgcccgc cagatccgct cagcgatgct
     9661 ggaacaattg agtgaagacc acattcgtac cgcccgggcc agcggcttgc ccggctggta
     9721 tatcgttcta tgttatgcgc tacccaatgc attgatccca tcgattaccg tattgggtct
     9781 ggcgctgggc gatttgttgt atggcgcagt gctcaccgaa accgtttttg cctggcccgg
     9841 aatgggtgca tgggtagtaa catcaataca ggcgctcgac ttcccggcag tgatgggctt
     9901 tgccgtcgtg gtttcatttg cttatgtgct ggtcaacctg gtggtggatt tgctctattt
     9961 gtggattgat ccgcgtatcg gacgtggagg tggtgaatga tgctaagcga ggatacgtcc
    10021 gccgtacgcc cagcaaaaca aacgcgattt aacggtgcaa aactggtatg gatgctgaaa
    10081 ggcagtccgc tcaccgtgac cggcgcagtc atcattgtat taatgctatt gatgatgatt
    10141 ttttcaccgt ggctggcgac gcatgatccc aacgccattg atttaaccgc ccgccttttg
    10201 ccgccttctg cggcgcactg gtttggcacc gatgaagtgg gacgcgatct gttcagccgc
    10261 gtactggtcg gcagtcagca atcaattctc gccggattag tcgtggttgc cattgcgggt
    10321 atgattggtt cgctactcgg atgtctatcc ggtgtgcttg gcggacgcgc agacgccatc
    10381 atcatgcgca tcatggacat tatgctgtcg attccttcgt tggtactgac aatggcactg
    10441 gctgccgctc tcgggccgag tttatttaac gccatgctgg cgattgctat tgtgcgaatt
    10501 cccttttatg tgcgcctggc gcgggggcaa gcattagttg tacgccagta tacctatgtt
    10561 caggcggcga aaacctttgg tgcgtcgcgt tggcatctga tcagctggca tattttacgt
    10621 aactccctgc cgccgctgat cgtgcaggca tcgctggata tcggtagcgc gattttaatg
    10681 gccgccacgt tgggatttat tggcctgggt gctcaacaac cgagtgctga atggggcgcg
    10741 atggtagcga atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc atttccgggg
    10801 acagcgattt tgcttaccgc cgttgggttt aatctctttg gtgatggtat tcgcgatctg
    10861 cttgacccga aagcaggagg aaagcagtca tgacccaacc cgttctggac attcaacagc
    10921 tgcatttgag tttccccggt tttaacggtg atgttcacgc gctcaacaat gtgtccttgc
    10981 agattaaccg cggcgaaatt gtcggtctgg tgggagaatc cggctcaggt aaatcagtca
    11041 ccgcaatgct gattatgcgt ctgctacaga cgggcagtta ttgcgtacat cgggggcaga
    11101 tttcactgct gggagacgat gttcttaacg ctcgggaaaa acagcttcgt cagtggcgcg
    11161 gcgcacgagt ggcgatgatc tttcaggaac cgatgaccgc cctcaatccg acacgtcgaa
    11221 taggtcttca gatgatggac gtgatccgcc atcatcaacc aataagtcgt cgggaagcca
    11281 gagctaaagc gattgccctg ctggaagaga tgcaaatccc ggatgccgtg gaagttatgt
    11341 cgcgctatcc gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt gcgctggcat
    11401 tctcctgcga gccgcaattg attattgccg acgaaccgac tacggcgctg gacgtcacgg
    11461 tgcagttgca ggtactgcgt ttgcttaaac ataaagcccg cgccagtgga actgcggttc
    11521 tgttcatcag ccatgatatg gccgtggtgt cgcaactgtg cgatagcgtt tacgtgatgt
    11581 atgccggaag cgtaattgaa agcggcgtga cggcagacgt tatccatcat ccccggcatc
    11641 cgtataccat tggtttgctc caatgcgcac cagaacatgg agtaccacgc cagctattac
    11701 ccgccattcc agggacggta ccaaacctca cccatttgcc tgacggctgc gcttttcgcg
    11761 atcgttgcta tgcggcaggt gcacagtgtg aaaacgtccc ggcgctgaca gcgtgtggtg
    11821 acaacaacca gcgctgcgcc tgttggtatc cgcagcagga ggtcataagt gtctgacacg
    11881 ttattaacgt tacgcgacgt ccatatcaat ttcccggccc gtaaaaactg gcttggtaaa
    11941 actacggaac atgttcatgc gattaatggt attgatttac agatccgccg tggtgaaacc
    12001 ttagggatcg tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct tttaatgggt
    12061 atgctgcaac cgagccacgg gcagtacatc cgttctggct cacaacgcat tatgcagatg
    12121 gtgtttcagg acccgctctc ttcgctcaat ccgcgcttac cggtgtggcg catcatcaca
    12181 gaaccgctct ggatagctaa gcgtagtagt gaacaacagc ggcgagcgtt ggcagaggag
    12241 ctggctgtgc aggtgggtat tcgtccggag tatctcgacc gcctgcctca tgcgttctcc
    12301 ggcgggcagc ggcaacgtat cgccattgcc agagcactct cttcgcagcc tgacgtgatt
    12361 gtgcttgatg agccaacctc tgcgctggat atctccgtgc aggcgcagat cctcaattta
    12421 ctggtaacgc tacaggaaaa tcacgggctg acctatgtgc tgatttcaca caatgtctcg
    12481 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtagaactg
    12541 ggcgacgcgc agcaggtgct gacggcacct gcacatccat acacccgatt attgctggat
    12601 tccctccccg ccattgataa accgctggag gaagaatggg cattacgtaa aacggatctg
    12661 ccgggaaacc gcacgttgcc gcaaggctgt tttttctacg aacgttgccc gctggcaacc
    12721 cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga gctccggtgc
    12781 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc
    12841 cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag
    12901 agaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac
    12961 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg gcatccactt
    13021 tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca
    13081 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    13141 ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    13201 cgccactctc ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct
    13261 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    13321 tacgtgagaa acagcacgag gataagcagg atattccgaa taaaatccgg cgaaaatatt
    13381 acttctgcac ttaaattaaa ttcccacaat agattaccgg gaatatataa cccaaaagat
    13441 attgtatcaa tattaatcgg atcataccaa tcacaatccc tatgaatatc atggtgttaa
    13501 aatataaaaa tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc
    13561 tgaatgtgct ttataaatct gcgatccgta gcagacacca taaatacaca gacacggaga
    13621 atcactatgt ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat
    13681 gccattgagc aaggccttcg ggcacagcac ggtgttgtca ctgaagatga cattctcatg
    13741 gagctgacca aatgggttga agcttctgat aacgacatcc tcagtgatat ctaccagcaa
    13801 acgatcaatt atgtggtcag tggccagcac cctacgcttt aaggtgctat gcttgatcgg
    13861 caacctaatt taggggttta gcacgtgttt cttcgctacg gcaatgttgt ccttaaaact
    13921 agctacagga ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac
    13981 tggaccataa aatttctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtag
    14041 taaataaccc aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc
    14101 aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact
    14161 tgaggccgac gccctggcgg taaagcaaag acgataaaag ccccccaggg atggatattc
    14221 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
    14281 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
    14341 aagaacgccg taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag
    14401 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
    14461 tctacctgcg taacatccag gacactaacg aaaccctctt ctaccgtctg gtaaacaatc
    14521 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
    14581 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cggcacaata
    14641 tggacgatat tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg
    14701 gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
    14761 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
    14821 tggatgtcgg aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta
    14881 atccgcgtat cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga
    14941 aacaacgctg gccagacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc
    15001 cgttacttaa ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg
    15061 cggcggtaac agtcggcaca ctgatcgcag caagccgcgc ggcaggtggt cagttaagcg
    15121 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
    15181 tctcccagac ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg
    15241 tcgatcgctt tggcttgctg actgacaaga tgccgaacct gctgcctttc cagaccaaac
    15301 tggtgcagaa gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ctgtcactgc
    15361 tggatgtggt gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc
    15421 tgtttacgga agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
    15481 cgctgtctaa cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggaccg
    15541 aaggtaacgc gctggtcgcc acgggcagcc cgtttaatcc agtggtatgg aaagataaaa
    15601 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta
    15661 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgctgg
    15721 cgcagtattc accattggtg ctgaacggcg aaggtatggt actgccggaa ctgaaagata
    15781 ttcagaaagt ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
    15841 tggcggtgaa aacctctgcc gaagccctgc aacaggccat tgacgataat ttctggcaag
    15901 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac
    15961 cgggctattt ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
    16021 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca
    16081 gttgttacga aggatcatca tgttgacgtt acgtataaaa cactgcgctc actgaaacat
    16141 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
    16201 aatggcgatt ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg
    16261 gcagaagtgg gtccaggtat cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
    16321 ttctacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
    16381 tcagttaaaa atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc
    16441 gccgattacg cggtaaaagt gccagatggt ctggactcgg cggcggccag cagcattacc
    16501 tgtgcgggag tcaccaccta caaagccgtt aagctgtcaa aaattcgtcc agggcagtgg
    16561 attgctatct acggtcttgg cggtctgggt aacctcgccc tgcaatacgc gaagaatgtc
    16621 tttaacgcca aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa
    16681 atgggcgcag atttagcgat taactcacac accgaagacg ccgccaaaat tgtgcaggag
    16741 aaaactggtg gcgctcacgc tgcggtggta acagcggtag ctaaagctgc gtttaactcg
    16801 gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg tcggtctacc gccggagtct
    16861 atgagcctgg atatcccacg tcttgtgctg gatggtattg aagtggtcgg ttcgctggtc
    16921 ggcacgcgcc aggatttaac tgaagccttc cagtttgccg ccgaaggtaa agtggtgccg
    16981 aaagtcgccc tgcgtccgtt agcggacatc aacaccatct ttactgagat ggaagaaggt
    17041 aaaatccgtg gccgcatggt gattgatttc cgtcactaag aggcctttgc tgcgactgcc
    17101 atgttcgggt cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg
    17161 cgtaggaagg aacaataatg atagtgttcc cagtgaagga acgctactga gagtaaagac
    17221 actaatgaaa atggccaatc atccccgccc gggggacatt attcaggaat cactggacga
    17281 acttaatgtc agcctgcgcg agtttgccag agcaatggaa attgctccct caacggcaag
    17341 ccgattgctg acaggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt
    17401 gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc
    17461 agaaaaaacg gtggatgtgt cgcgattacg ccgtctggtt acgcaataaa aaaatggcac
    17521 cttataaagc gccatttgaa actgatgaca aatgcaacat tgcccgatgc gctacgctta
    17581 tcaggcctac acgtttactg caatctattg gatttgaacg gtttgtaggc cggataaggc
    17641 gttcacgccg catccggcat gaacaaagcc tacgttgtct acaatatgaa aatggcgcct
    17701 tttacagcgc cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt
    17761 ttttcgcttc tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc
    17821 gacggctcac cttcccttcg atcatccctt taatcgatcc tttcacccag aatgccatat
    17881 acatatggat caggatggcg tggatcagga tgataccggc agccgcgtgg atcagcaggc
    17941 tgtagcgaac aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc
    18001 cggtcaccaa cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg
    18061 cgttgtactt accgacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt
    18121 tcaacagcca cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca
    18181 taaacatcag cgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaatg cgtcccatct
    18241 gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg aaagaagaac gaaatcccgg
    18301 acagcgccac caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt
    18361 tggtgcgcac aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc
    18421 ttattcgggc caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca
    18481 gccgctgcca gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga
    18541 tctttcggca gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg
    18601 tgcgtaccac cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc
    18661 agtttcgcca cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata
    18721 gccccggtcg gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg
    18781 cagagcgtgc atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga
    18841 cacccggcaa tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg
    18901 ttagcgtact gaatgattgc accagcagac gggcacgcca tcaggcagcc cggatcttca
    18961 cagtgcatac agccgtcttt acggatcagc cactccagct tgccgttctg ttcggtttcg
    19021 ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaactccg
    19081 acgcagtgcc ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct
    19141 ttacaaccga tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc
    19201 acctgagaag gcggcgtgat ggagttagtt gcggaccttt tgataatgtc ctgcgtttcc
    19261 atagccatct gttcgccccc ttacgccttc tcgatgttta ctaagaacgc tttatattcc
    19321 ggcgtttgcg agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta
    19381 cgcgcgacac cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg
    19441 tttacattca gcggtttcag acggcgcgtt accactgcca ccgcgcggat aaagcctcgc
    19501 ttgctggaga cagtgacacg atcgccatta ttaatgcctt tcgccgccgc cagcgtttcg
    19561 ctgatttcca caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag
    19621 gtgtggaagt gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta
    19681 cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga
    19741 tgcagagggt tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct
    19801 tccgccattt tgttgatggc aaacaggcgt cccatccctt ccggctgcat aataaacggc
    19861 ccagttggcg tacccggtgc ggcattgccg aagtcaggaa tatcgttacc cgtccacttg
    19921 ctgccgttcc actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc
    19981 gaagcacggt tgtacagcac gcgacggttg agcggccacg cccaggccca tcccagcgta
    20041 ttccccagac cggacgggtc ggagttatca cggttagcca tctggttgcc ctgctctgtc
    20101 cagctaccgg tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca
    20161 aagctactca gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct
    20221 tccagcgcat agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc
    20281 ttgtagttcc agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac
    20341 aactcgcgca gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc
    20401 ggcgcgtcct gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca
    20461 tcctcttcag caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga
    20521 tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag aggtttcagt caccagcgga
    20581 tcgataacca ccatgtactt cagcttgctc aggcagctca ccactttgtt tttgtccggg
    20641 aaggacgcaa ccgggttaaa gccctggcag aaataaccgg tgactttgcc ttcatccatc
    20701 atgttgaaat acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc
    20761 cagttgttct ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac
    20821 ttcggatagt tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct
    20881 tccagatacg actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg
    20941 ctggtagaga gcaggcctaa gtcagtcagg ccctgaatgt tggagtggcc acgcaatgcg
    21001 ttcacgccgc caccggccat acccatgtta ccgagcagca actggatcat cgccatagta
    21061 cggatgttct gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt
    21121 gtgcgatccg gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt
    21181 ggcgtaccgc agatgttttc aacgacgtcc ggcgtgtagc gggaaacgtg cgctttcagc
    21241 aggttccaca cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca
    21301 tcgaactgat agttccagga cgacttatcg tactggcgtt tttcagcgtc gtagccgctg
    21361 aacagaccgt cttcgaaagc aaaatcatca cgcaccagca ggctggcgtt ggtgtaatgc
    21421 ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac gccagacagg
    21481 aacgtaatgt ccgtaccgga acgaataggc gcgtaaatat ccgccacaga agcggtacgc
    21541 gtaaaacggg gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc
    21601 cagcggaaac cgacgggatg cgcttcagca gcgttaccgc ccatcaccat cacgacgtta
    21661 gcgtttttga tatccaccca gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga
    21721 cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg
    21781 gagcgggcaa atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc
    21841 ataccggtag aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta
    21901 gcgtcacggt cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg
    21961 cgctgccatt tgtcagaacc tggcgcacga tattccgggt agcgcagacg gttttcgctg
    22021 ttgacgtaat ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga
    22081 tccgggtcac cttcaatgtg ataaatcgct tctctggcgt ttttcgcgcc atcacccagg
    22141 ctatacatca atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta
    22201 gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat
    22261 gccgctaccg ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg
    22321 acgtccattg ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa
    22381 ttctgaatct ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct
    22441 gcgcaaattg acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt
    22501 aaaaaaatgc tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca
    22561 tgacacgaca aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg
    22621 taggattgat tcgcggtgtc agcgaggggc tcggcccggt cggcggcgca gctgctatct
    22681 attcattaag cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc
    22741 cgaaaggcta tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tgtctggcgc
    22801 tttccttagg gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc
    22861 tgtggcccag cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc
    22921 tgattgtacc aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga
    22981 atgggttaca ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc
    23041 tggcgtttat tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac
    23101 gcggatttaa cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact
    23161 attttctcac gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct
    23221 ctgcggcatt taccttagga tttgcttatg ctgcatggaa tgtcggtata ttgcatggca
    23281 atgtcaccat aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag
    23341 ccgtgctgct cagcgccccg ctgtcgttct cgttctggca agg
//
LOCUS       DAOFQD010000070        23238 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.070, whole genome shotgun sequence.
ACCESSION   DAOFQD010000070 DAOFQD010000000
VERSION     DAOFQD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.070"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1321)
                     /locus_tag="QBF46_002841"
     CDS             complement(<1..1321)
                     /locus_tag="QBF46_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6675002.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTI
                     ASWDDVALAVVAHPNADNAMQTTADFSNSTLMGDVIFSSNFDENFFPRGADSYRDADG
                     EVDTNGWDGTDRLDLTLNNGSKWVGAAQSVHQTGSIDVDGDGKGDIATYGVGTEATAT
                     LIDIEDNSLWPLSTVGVENDDTSYSEFDHITGNQVYQSGLFNVTLNTG"
     gene            1844..2818
                     /gene="hemB"
                     /locus_tag="QBF46_002842"
     CDS             1844..2818
                     /gene="hemB"
                     /locus_tag="QBF46_002842"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDQ6675003.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFAMDLAEKKILR"
     gene            complement(2976..3827)
                     /gene="tauD"
                     /locus_tag="QBF46_002843"
     CDS             complement(2976..3827)
                     /gene="tauD"
                     /locus_tag="QBF46_002843"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDQ6675004.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3824..4651)
                     /gene="tauC"
                     /locus_tag="QBF46_002844"
     CDS             complement(3824..4651)
                     /gene="tauC"
                     /locus_tag="QBF46_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDQ6675005.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAARSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(4648..5415)
                     /gene="tauB"
                     /locus_tag="QBF46_002845"
     CDS             complement(4648..5415)
                     /gene="tauB"
                     /locus_tag="QBF46_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDQ6675006.1"
                     /translation="MLQISHLYANYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQNNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(5428..6390)
                     /gene="tauA"
                     /locus_tag="QBF46_002846"
     CDS             complement(5428..6390)
                     /gene="tauA"
                     /locus_tag="QBF46_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6675007.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            7006..7677
                     /locus_tag="QBF46_002847"
     CDS             7006..7677
                     /locus_tag="QBF46_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDQ6675008.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            7687..8883
                     /locus_tag="QBF46_002848"
     CDS             7687..8883
                     /locus_tag="QBF46_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDQ6675009.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSHFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            8861..9442
                     /locus_tag="QBF46_002849"
     CDS             8861..9442
                     /locus_tag="QBF46_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDQ6675010.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAFIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            9444..10217
                     /locus_tag="QBF46_002850"
     CDS             9444..10217
                     /locus_tag="QBF46_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDQ6675011.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            10403..10678
                     /gene="frmR"
                     /locus_tag="QBF46_002851"
     CDS             10403..10678
                     /gene="frmR"
                     /locus_tag="QBF46_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDQ6675012.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            10713..11822
                     /gene="frmA"
                     /locus_tag="QBF46_002852"
     CDS             10713..11822
                     /gene="frmA"
                     /locus_tag="QBF46_002852"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDQ6675013.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            11915..12748
                     /gene="fghA"
                     /locus_tag="QBF46_002853"
     CDS             11915..12748
                     /gene="fghA"
                     /locus_tag="QBF46_002853"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDQ6675014.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMHHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(12873..13412)
                     /locus_tag="QBF46_002854"
     CDS             complement(12873..13412)
                     /locus_tag="QBF46_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDQ6675015.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(13514..14725)
                     /gene="mhpT"
                     /locus_tag="QBF46_002855"
     CDS             complement(13514..14725)
                     /gene="mhpT"
                     /locus_tag="QBF46_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDQ6675016.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLVRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQATGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(15101..16114)
                     /gene="dmpG"
                     /locus_tag="QBF46_002856"
     CDS             complement(15101..16114)
                     /gene="dmpG"
                     /locus_tag="QBF46_002856"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDQ6675017.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(16111..17061)
                     /gene="mhpF"
                     /locus_tag="QBF46_002857"
     CDS             complement(16111..17061)
                     /gene="mhpF"
                     /locus_tag="QBF46_002857"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDQ6675018.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(17058..17867)
                     /gene="mhpD"
                     /locus_tag="QBF46_002858"
     CDS             complement(17058..17867)
                     /gene="mhpD"
                     /locus_tag="QBF46_002858"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDQ6675019.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(17877..18743)
                     /gene="mhpC"
                     /locus_tag="QBF46_002859"
     CDS             complement(17877..18743)
                     /gene="mhpC"
                     /locus_tag="QBF46_002859"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDQ6675020.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(18761..19705)
                     /gene="mhpB"
                     /locus_tag="QBF46_002860"
     CDS             complement(18761..19705)
                     /gene="mhpB"
                     /locus_tag="QBF46_002860"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDQ6675021.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(19707..21371)
                     /locus_tag="QBF46_002861"
     CDS             complement(19707..21371)
                     /locus_tag="QBF46_002861"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDQ6675022.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LNTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            21448..22395
                     /gene="mhpR"
                     /locus_tag="QBF46_002862"
     CDS             21448..22395
                     /gene="mhpR"
                     /locus_tag="QBF46_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDQ6675023.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHHVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQDRKELIEMLASRPGDDYQLAR
                     EPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGMHLR"
     gene            22472..>23238
                     /gene="lacI"
                     /locus_tag="QBF46_002863"
     CDS             22472..>23238
                     /gene="lacI"
                     /locus_tag="QBF46_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDQ6675024.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGACVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRA"
BASE COUNT         5619 a         6316 c         5804 g         5499 t
ORIGIN      
        1 aaccggtatt cagggtcaca ttgaacagac cgctctggta aacctgatta ccagtaatat
       61 gatcgaattc actgtaactt gtatcatcgt tttcaacacc aacggttgat aacggccaca
      121 ggctattatc ctcaatatca atcagagttg cagttgcctc ggtgccaaca ccgtatgtag
      181 cgatgtcacc tttaccatct ccatcaacat caatagaacc agtttgatga acagattgtg
      241 ctgcccctac ccatttacta ccgttattta atgtcagatc taggcgatct gttccatccc
      301 aaccgttggt gtctacttca ccatcagcat cgcgataact atctgcacca cgtgggaaga
      361 agttttcatc aaagttgcta gagaagatta catcgcccat caaagtagag ttactaaagt
      421 cagcggtggt ctgcatagca ttatcagcat tcggatgtgc aactacagct aatgcaacat
      481 catcccagct tgcaatagtg ctatcagcaa caccatctcc gtcagtatct acggtgacaa
      541 agttagattt accactataa tcgctggcat tacctgtatt accgaaccaa ccagtagtac
      601 cttcatcagt ccatgaacca gaggttacgg tagaatcttt aacagtgatg gtgttattaa
      661 acacttcgct gctgtttacg ccagtgctga cagtgttgtc gtcaatatct tcccactcat
      721 aaccctgagt caacgtaata cccgcaacgt gagagttatt agtgatggac agatcaactt
      781 cctgatccag agtaatagcc gtaccaatgt tgtaaacgtc tacaacatga gtatcagcgg
      841 cattattgta agtgccgtta tatgtgtagt gttcgtagtt gtcgtcgatc gttgaattat
      901 caacagtcag cgccagacgg tcatggtagt aatcatccgc acgatcagca caatcagttg
      961 tcatgcactc ggaataaatc ataccgtgaa tagtgctgtt tttgattgtc agactattgg
     1021 cgttggtatt ggtagaaata ccgtcgtcca gatagtaggt agaaatcacg ccgttaacgg
     1081 tagcgttatt aatgaccggg tagatatcgc cgttgtaata gctatcagcg gcatagttgt
     1141 tccagcctac ataaccatca tagtaaacgt catcagcata ggtggcgtcg ttgtagtgat
     1201 ggaaagtatt gtaagtttga ccagaaatat cgttggtcgc tgcattagcc tgagaggtga
     1261 tagccagagt gcaagccaat gctaattgtg atactacaag tttctttttc caggagtgca
     1321 tttgtcatcc ctcctcaggg acgtaataag ttgatccatc aattatcaat gcatagaaaa
     1381 catattatgt ttcgcgctaa cgattatgct gtgttacaga cgaatggttc aattattctt
     1441 tcccctaagt ccgaatgtgg acatatataa tttatggaga aaaacacgca tcgaattgat
     1501 aaaaacacca tatttcaata tgttgcagat gatgttattt ttgatacata cgcgtcacaa
     1561 atatcacaga cacaaaccag ataaatatcc tatcaatatg caacaaagtt ttaattattc
     1621 aatttatagc agggcaacca aactcccaaa ataataacca agagctatta ataaagatta
     1681 aaaatatttt cagtgaatat gaaattattg ctatttctat tacaaagatg tcctggtctc
     1741 tctttcattc tcttcttgtc aacatcgcga caactttcgt aaaacactcc tgccccgctt
     1801 caggtatact gtgcacctta attccacaaa catcaggcag accatgacag acttaatcca
     1861 acgccctcgt cgcctgcgca aatctcctgc gctgcgcgct atgtttgaag agacaacact
     1921 tagccttaac gacctggtgt tgccgatctt tgttgaagaa gaaattgacg actacaaagc
     1981 cgttgaagcc atgccaggtg tgatgcgcat tccagagaaa catctggcac gcgaaattga
     2041 acgcatcgcc aacgccggta ttcgttccgt gatgactttc ggcatctctc accataccga
     2101 tgaaaccggc agcgatgcct ggcgggaaga tggactggtg gcgcgaatgt cgcgcatctg
     2161 caagcagacc gtgccagaaa tgatcgtcat gtcagacacc tgcttctgcg aatacacatc
     2221 tcacggtcac tgcggtgtgc tgtgcgagca tggcgtcgac aacgacgcga ctctggaaaa
     2281 tttaggcaag caagccgtgg ttgcagctgc tgcaggcgca gacttcatcg ccccttctgc
     2341 cgcgatggac ggccaggtac aggcgattcg ccaggcgctg gacgctgcgg gctttaaaga
     2401 tacggcgatt atgtcgtatt cgaccaagtt cgcctcttcc ttttatggtc cgttccgtga
     2461 agctgccgga agcgcattaa aaggcgaccg caaaagctat cagatgaacc caatgaaccg
     2521 tcgtgaggcg attcgtgagt cactgctgga tgaagcccag ggcgcagact gtctgatggt
     2581 taaacctgcc ggagcgtacc tcgacatcgt gcgtgagctg cgtgaacgta ctgaattgcc
     2641 gattggcgcg tatcaggtga gcggtgagta cgcgatgatt aagttcgccg cgctggcggg
     2701 tgctatagat gaagagaaag tcgtgctcga aagcttaggt tcaattaagc gtgcgggtgc
     2761 ggatctgatt ttcagctact ttgcgatgga tttggctgag aagaagattc tgcgttaatt
     2821 ttgtttcctc tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc
     2881 ctaaccctct ccccaagggg gcgaagggac tgtccgtgct cgcttcatct atatcaccgt
     2941 tctttcgccg ctcgaggtga ccgtccactc tcgtatcacc ccgcccgata aaacggttta
     3001 tccccaagaa tcgtcgcccg atgcataatc cgtcgctgtg gcaggtaatc ggcattggca
     3061 tagtgctggg tcacgcggtt atcccaaatc gcaatatcat ttggttgcca gcgccagcgc
     3121 acctgaaact ccggtttagt gatatgagcg aataaaaaac ttaacaaggc ttcgctctct
     3181 ttctcgctca catcaacaat tcgcgtagta aagccttcat tcacaaacag cgcctgttta
     3241 ccgctcaccg gatgcgttcg caccaccgga tgtagcaacg gcggattttt cgcgaccgcc
     3301 tcgcgccagc gttgatgttc ctcctcggtt ttgcggtatt tgtattccgg gaacgattta
     3361 cggaaatcat gctccgcacg cagcccgctc agcagctggc ggaagggaac agagagcgcc
     3421 tcataggccg caataccgct ggtccagagc gtatcaccgc cggtcgaagg taactcttta
     3481 gctgccagaa tcgcccctgc gggtggcgtt tcaataaatg tcacatcggt atgccagttg
     3541 tcgttatccg gcggattatc gttatgggta tccagcacga tgatctcgtc aaccccttcg
     3601 gcatgagggt aaacagggtg aatatgcagt tcgccaaaac gctgggccag cgcgcgttgc
     3661 tgctgcggcg taatagcttg atcgcgcaga aataccacct gatggcgcag caccgcatgg
     3721 taaagctgtt caaactgatt atcgcttaac gggcgcgtca ggtcggcacc cgaaatttgt
     3781 gcgccgatat acggccccag cggggtaatg ctcaaacgtt cactcattgt acttctccat
     3841 gccagggcgt caggcggcgc tgtaatgcgc gcagacccag ttctaaaaga aaggcgataa
     3901 tcgcaatcac cgcgatcccc gccagcacca cgtcagtcgc gagaaattca cccgctgact
     3961 gaaccataaa tcctaaaccg cgcgttgcgg caatcagctc cgccgccacc aacgtagacc
     4021 agcccacacc cagaccaatg cgtaatccgg tgaggatttc cggcagcgcg ccgggcaaaa
     4081 tgacaaacca cagcacctgc gcacggctgg cacccagcga ccgggcggca cgaatgcgaa
     4141 cctgctgcgc gcttttcacc cccgccagcg ccgacattgc caccggcgca aaaatcgcta
     4201 aatagatcag taagatcttc gaggtttcgc caataccaaa ccaaatcacc atcagcggca
     4261 aataagccag cggtggcacc ggacgataaa gctcgattat cggatccaga atgccgcgca
     4321 ccgtcgggct tagccccatg gcgatcccga ccggaatacc gattaccacc gccgcgagta
     4381 gcgccagcac aatgcgcgtc aggctggcgg ccagatgctg ccacagcgtg gcgtccataa
     4441 atccctgcgg cccggcaatg gtgagcagtt ttgccagtac ctgttgcggt ggcggcaaaa
     4501 acagcgggct aatcagttgc agcgctgcga ctgcccacca tacagtgagc aaaaccgcta
     4561 acgtgccaat gcttaaggtc acctgacgcg agagcggcca gcgccatttc agccgatgcg
     4621 aatgcagttt ttcattaatg agcacactca tgagaacgcc tcccgctgct caaatacgcg
     4681 gcttaaaaca tattcgcgca tggcgatgaa ttgtggatcg gacttgatgc tgcggctcga
     4741 ctctcccgca acaaagcggc gagcaaagtt gagcggcagc cgctccagca cgcggccagg
     4801 gccgggtgaa agcagaacca gttcagtcgc cataaacacc gcttcttcta tatcgtgggt
     4861 aatcaacagc acctgcttgc ccgtctcctg ccagagtttc agcagcaggg tttgcatctg
     4921 gtcgcgggtg aaggcatcca gcgcaccaaa cggttcgtcg agtaataaca gctggggatt
     4981 tgccgccagc gcgcgagcaa tgcccacccg ctgacgttga ccgccggaga gctgccagat
     5041 gtagcgtttt tctgctcctt ccagccccac ttttttcagc atctggtgcg cgatttccag
     5101 tcgctgcatt ttctctatac ctgccagttg caggccgaac gccacgttgt tctggacatt
     5161 gcgccacggt agcagccctt cattctgaaa aaccacgccg cgctctgctc ccggcccctc
     5221 aatacgctta cccgccagtt gaatgctgcc gtgctgataa ggcacaaaac cggcaatcag
     5281 attcagcagg gtggttttac cgcagccgga cggccccagc accaccagta gctcaccgct
     5341 ttccagcgtc aggttgatat cttccagtgc cggtttgccg ccataattgg cgtaaagatg
     5401 agagatttgc agcatctgcg cctcctttta ttgcacgaag cgcgaggtaa cgtactggct
     5461 gtaatcattc gctacagccg ggaccttgcc ctgctctttc aaaaactgcg cggtgtcgat
     5521 gatcgctttg ttcaccggtc cggtcagttc tgccgtttgt tgctgcggcg tcagataggt
     5581 attccccttc accagccccg gaacgtcacc ttcaggcacg ccgcttaacc gcgccagttt
     5641 gctgatgttt tccggctgtt tcagccacgc gtctgggtta gcaatgtacg gttgctgagc
     5701 atcgatggcg cttttggcga acgctttcac cacctcagga tgtttctcgg caaaatcttt
     5761 gcgcaccacc cagacgtcca gcgttggcgc accccactgc ccgacctgtt cagaatcggt
     5821 cagcactttg ccgtcttttt ccagggcgtt aaccgccggt gcccagacat aagcaccatc
     5881 aatatctccc cgctgccatg cagcgataat cgcgggcggc tgcaggttca caatctccac
     5941 ttgcccaggt ttaatgcccc agtgtttcag cgctgccagc aggctgtagt gggtggtaga
     6001 gataaacggt acggcgatgc gtttgccaat cagatcttcc ggtttgctga tagttttctt
     6061 taccaccagc gcttctgagt tacccagttt tgacgccagc aagaagactt caatcggcac
     6121 ctgttggctg gctgcaaccg ctaacgggct ggaaccaagg ttgccgattt gcacgtcgcc
     6181 agaagccagc gcccgcacga tgctggctcc gctgtcaaac ttacgccagt ccacggttgc
     6241 tccgctttct ttagcaaagg tgttgtcggc ctgagccact ttcgccggtt cggctgaggt
     6301 ttgatacgcc acggtgacgt tcaccgcctg tgcctgaaaa gcgatgaatg ccagtgcggc
     6361 aagaagtgtg tttcgcgatg aaattgccat gattgtctgc tcccctgtct tgttatggga
     6421 gcagtattca ggaataaaaa cattcattaa aagaattagt cgttatcgca cagatgattt
     6481 tattcttagc aaaaaaacgg ctaaggaaag attaaatatg caattactcc gttctaacaa
     6541 ttggggaaat tactacactt cattttagtg atgcataaac aaccacatta cgagatttat
     6601 tacttcatta tttctatcaa tatcaataca tataataaaa ccattatatt cgcattgtat
     6661 atatcactgt atcattctca atcactcgca tattacattc acataatgta tataaacaat
     6721 gcttgttcac agaacatatt ttttaaatgc aattaattga caaactaaat cacatctaaa
     6781 tacaatgtat aaaacaaaat aactatacat accaaacata caaacactta acatatgaat
     6841 tgcatagaaa cattacccag tcgaacccgc gggtctgaaa gcccttgctt ttcagttatt
     6901 agcaacattt aacatttata aattaacatc tggctgcgcc agagattacg tctctgagtg
     6961 catcgttgtg cttgtagtat aataaataaa atggagtgtc attaagtgga taaggtttta
     7021 gattcagccc tcctttcttc ggcaaataaa agaaagggta tccttgctat tggcgcacat
     7081 cctgacgata tagaattagg ctgtggcgca tcgcttgctc gtcttgcgca aaaaggaatt
     7141 tatatcgcag ccgtggtgat gactaccggc aactctggca cagatggaat aatcgatcgc
     7201 catgaagaat cgcgcaacgc cctaaagata ttagggtgcc accaaactat tcatcttaat
     7261 tttgctgaca cccgcgctca tttacagctc aatgatatga tttccgccct ggaagacatc
     7321 attaaaaatc aaattccttc tgatgttgaa atcatgcggg tatataccat gcatgatgcc
     7381 gaccgccatc aggatcatct cgctgtttat caagcttcaa tggttgcctg ccgcactatt
     7441 ccacaaattc tcggctacga aaccccaagt acctggcttt catttatgcc tcaggttttt
     7501 gaatccgtta aagaagaata tttcacagtc aaacttgctg cattaaaaaa acataaaagc
     7561 caggaacgac gcgattatat gcgtcatgat cgcctgcgtg cagttgcaca atttcgcggg
     7621 caacaggtca atagcgatct gggtgaaggc tttgtaattc ataaaatgat tctttgagca
     7681 aattgcatga aaacctggat atttatctgt atgtccatag caatgttgct atggttttta
     7741 agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta ttgacgccat tatacctgcg
     7801 tataacgaag gcccgtgtct ggcgcagtca ctggataatc tactgcgtaa cccttatttt
     7861 tgccgggtaa tttgcgttaa cgacggctcc acggacaata ccgaagcggt catggcggaa
     7921 gtcaaacgca aatggggcga ccgctttgtt gccgtcacgc aaaaaaatac cggtaaaggt
     7981 ggtgcgctga tgaatggcct caattatgcc acctgcgacc aggttttttt aagtgatgcc
     8041 gacacctatg ttccgcccga tcaagacgga atgggctata tgctggcaga aattgagcgc
     8101 ggtgctgatg ccgtaggcgg cattccctct actgcgttga aaggcgcggg gctgttaccg
     8161 cacatccgcg cgaccgtaaa gttgccgatg attgttatga agcgcacgct acagcagctc
     8221 ctgggcggcg caccgtttat tatcagcggt gcctgcggaa tgttccgtac tgatgtattg
     8281 cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc ttgatctcac ctggacatta
     8341 gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca tcgtataccc acaggaatgc
     8401 aacagcccgc gtgaggagtg gcgtcgctgg cggcgttgga ttgtgggata cgcggtctgt
     8461 atgcgcctgc ataaaagact tttatttagc cacttcggta tcttcagtat atttcctatg
     8521 ctgttggttg tgctttatgg cgttgggatt tatctcacta cctggtttaa tgaattcatc
     8581 accaccgggc cgcatggtgt ggtgttggca atgtttccgc ttatctggat cggcgtagtt
     8641 tgtgttattg gtgcttttag cgcctggttt catcgttgct ggttgttggt gcctttagcg
     8701 ccgctttccg ttgtgtatgt attattagct tatgccatct ggattattta tggacttatt
     8761 gcctttttta ctggacgcga acctcagcgc gacaaaccca cccgctattc cgcactggtg
     8821 gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa ctgaaaaact atctgaagct
     8881 taatgttaaa gagaaaaata ttcagattgc agaccaggta attattgatg aaactgcagg
     8941 tgaagttgtt atcggcgcga atacccgtat ttgtcatggt gcctttattc agggtccggt
     9001 agtgattggc gcaaactgcc tgataggtaa ttatgcgttt attcgtcctg gcacaataat
     9061 cagcaatggt gtaaaaattg gttttgccac cgaaattaaa aatgcggtta ttgaagcgga
     9121 agcaacgatt ggtccgcaat gttttattgc cgactcggta gttgcaaacc aggcatattt
     9181 gggcgcacaa gtacgtacca gtaatcatcg tctggatgaa cagcctgtgt ctgttcgaac
     9241 tccagaggga attatcgcta ccggatgcga taaattaggt tgttatatcg ggcagcgttc
     9301 acgccttggc gtacaagtta taattttgcc tgggcgaatt atttccccga acacgcaact
     9361 tggcccgcgc gtgattgtag aacgtaattt acctgccgga acttattcac tccgacaaga
     9421 acttatccgt acaggagatt aaaatgataa aacggacgtt attagcggcg gccattttta
     9481 gcgcattgcc cgcttatgcc gggttaactt ccattaccgc gggctacgat tttaccgatt
     9541 attctggcga tcatggcaac cgtaatttag cgtatgctga actggtggcg aaagttgaaa
     9601 acgcaacgct gctttttaat ctttcccagg ggcgtcgtga ttatgaaact gaacatttca
     9661 atgccactcg cggtcagggt gcggtctggt ataaatggaa taactggctg acaacccgaa
     9721 cgggtattgc ctttgcggat aatacgccgg tctttgcccg ccaggatttt cgtcaggata
     9781 ttaacctggc cctgttgcca aaaacgcttt tcacgaccgg ttatcgctac actaaatatt
     9841 acgatgatgt cgaagtcgat gcctggcaag gcggcgtatc actctatact ggcccggtga
     9901 tcaccagcta ccgctatacc cattatgact ccagcgatgc aggtggtagt tatagcaata
     9961 tgatttccgt gcgtctgaat gacccgcgcg gcactggtta tacgcaacta tggctaagcc
    10021 gcggaacagg cgcttacacc tatgactgga cgccagaaac acgctacggc agcatgaaga
    10081 gcgtcagtct gcaacgtatt caaccgctga ctgagcaact taatctcggg ctgacggcag
    10141 gtaaagtgtg gtacgacacc ccaaccgatg attataacgg tctgcaactt gcagcccatc
    10201 tgacctggaa attctgattc cttctgccgc ccgctatccg gggcggcctt ccctgccgat
    10261 tagccccccc ctttcctctt tgttttccga ccacattcac cggataaatt ttattctcca
    10321 gtgttatata ctataggggg gtatgcattg acatatagca taccccccta tagtatattg
    10381 cgtgcagata atgaggtgcg aaatgcccag tactccggaa gagaagaaaa aggtccttac
    10441 tcgagttcgt cgtattcgag ggcagattga tgctctggaa cggtcgctgg agggtgatgc
    10501 cgaatgccgt gccatactcc aacagatcgc tgccgttcgg ggcgcggcta atgggctgat
    10561 ggcagaagtg cttgaaagcc atatccggga aacgtttgac cgaaatgact gctacagccg
    10621 cgaagtcagc caatccgttg acgacactat tgaactggtt cgagcctatc ttaaatagct
    10681 gaatctatta ccatattgag gaagagcgag agatgaaatc acgtgctgcc gttgcatttg
    10741 ctcccggtaa accgctggaa atcgttgaaa ttgacgttgc accaccgaaa aaaggtgaag
    10801 tgctgattaa agtcacccat accggcgttt gccataccga cgcatttacc ctctccgggg
    10861 atgacccgga aggtgtattc ccggtggttc tcggtcacga aggggccggc gttgtggttg
    10921 aagtcggtga aggcgtaacc agcgtcaaac ctggcgacca tgtgatcccg ctttacaccg
    10981 cggagtgcgg cgagtgtgag ttctgtcgtt ctggcaaaac taacctctgt gttgcggttc
    11041 gcgaaaccca gggtaaaggc ttgatgccag acggcaccac ccgtttttct tacaacgggc
    11101 agccgcttta tcactacatg ggatgctcaa cattcagtga atacaccgtg gtcgcggaag
    11161 tgtctctggc caaaattaat ccagaagcaa accatgaaca cgtctgcctg ctgggctgtg
    11221 gcgtgaccac cggtattggc gcggtgcaca acacagctaa agtccagcca ggtgattctg
    11281 ttgccgtgtt tggtcttggc gcgattggtc tggcagtggt tcagggcgcg cgtcaggcga
    11341 aagcgggacg gattatcgct atcgatacca acccgaagaa attcgatctg gctcgtcgct
    11401 tcggtgctac cgactgcatt aacccgaatg actacgacaa accgattaaa gatgtcctgc
    11461 tggatatcaa caaatggggt atcgaccata cctttgaatg catcggtaac gtcaacgtga
    11521 tgcgtgcggc gctggaaagt gcacaccgcg gctggggtca gtcggtgatc atcggggtgg
    11581 caggttctgg tcaggaaatc tccacccgtc cattccagtt ggtcactggt cgcgtatgga
    11641 aaggttccgc gtttggcggc gtgaaaggtc gttcccagtt accgggtatg gttgaagatg
    11701 cgatgaaagg tgatatcgat ctggaaccgt ttgtcacgca taccatgagc cttgatgaaa
    11761 ttaatgacgc cttcgacctg atgcatgaag gcaaatccat tcgaaccgta attcgttact
    11821 gatttcccgc aggtataccc cgtccacttc agacggggtt cttaatactc tccctgggca
    11881 gccgtccggg ggattaaccg tgagataatg actgatggaa ctcattgaaa aacatgccag
    11941 ctttggcggc tggcaaaatg tgtatcggca ttattcccaa tcactgaaat gtgaaatgaa
    12001 tgtcggcgtc tatctcccac caaaagccgc gaatgaaaaa ttgccggtgc tgtactggct
    12061 ttcaggcctg acctgcaacg agcagaattt cattactaaa tcggggatgc agcgttacgc
    12121 ggctgagcac aacattattg ttgttgcgcc ggacaccagt ccgcgaggca gtcatgtcgc
    12181 agatgctgac cgttacgatc tcgggcaagg tgccgggttt tacctgaacg cgacgcaagc
    12241 gccgtggaat gaacattaca aaatgtatga ctatatccgc aacgagctgc cggatttagt
    12301 gatgcatcat tttccggcaa cggccaaaaa gtctatctct ggtcattcta tgggcgggct
    12361 gggcgcgctg gtgctggcgt tacgtaaccc agatgaatat gtcagcgtct cggcgttttc
    12421 gcccattgtc tccccatcgc aagtaccgtg gggacagcaa gcctttgccg catatcttgg
    12481 tgaaaataaa gatgcctggt tggattacga tccggtgagt cttatttcac aaggtcaacg
    12541 cgttgcggaa atcatggttg atcaggggtt gagtgatgat ttttacgcgg aacagctgcg
    12601 gactccaaat cttgaaaaga tctgccagga gatgaatatc aagacgttaa tccgttatca
    12661 cgagggttac gaccacagct attattttgt ctccagtttt attggcgagc atattgccta
    12721 ccacgccaat aaactgaata tgcgttgata atagtgcacg actgccggat gcggcgtgaa
    12781 cgccttatcc ggcctacact tcgcccgtaa accgtaggcc tgataagacg cgcaaagcgt
    12841 cgcatcaggc atggcgcacg acttatctat gtttaccaca tcaaatcatc aggcactttg
    12901 aagtcggcat acggatcgtc ttcatcctgc tcttcggcac tgagcgcgct gtgcaacaca
    12961 atactgctgg catcacgctg agcaatttta tcggcaacac tggcgggaat aatggtgtat
    13021 tcaccttcgc tattgttatc gaccaacaag cgggcaatcg ccagacgacc attaatcaac
    13081 tgcgcctgtg tgagcttatc gacaaaaatc ttcttaatca gattgccgtc agtgaagtta
    13141 aaaccaatat cgccattggc aatggtgatt cggttcattt caatgagctg cttcacctgg
    13201 gctttatatt ctttcgccaa cgccgcttgt ttttgctgtt cgctaagctg tttatcacgc
    13261 tcaagctgtg cctttttatt ttcttctacc gcagcccgag cttcacgcgc ctgaacgcgc
    13321 gattttttcg ccgttctctc gaccttcgcc gcttttttgc tggtgactaa tcctgctttg
    13381 agcaactgct cttgtaaggt aagttttgcc atctttgttt ccaaacccgc tgaataattt
    13441 ttagcgagta tacctgtaaa cgccgtgggt gtattggggg caagtccctt tcgcccctgc
    13501 ttaataaggc gcgtcaggca tcggcacacg gctgcattct tgatctccgg ctcatcaaaa
    13561 taaacaccgc caacccagca acaagaatac ccggtgcaga agcggccatt acgccgaccg
    13621 tgccagtgcc taatgccagc attttcccgg ccagtaacgg accgctcata gcccccagac
    13681 gccctaccgc cacggctgtt cccacacctg ttgcgcggat ctgcgaactg taaaacaacg
    13741 gtgccagggc atacaaaacg ctttgcccac ctgtcgcaaa caaccccgcg acaaatcccg
    13801 ccagcaacat accgttaaat gacgacaccg ttccaagcgc cagcagcgaa gctaacatgc
    13861 cgctataaat cagtagcgac atggttactg gacgcagctt atccatcaat gcgcccaaca
    13921 ttaacgtccc gcttgccgcc cccatttgca gggcaaacat cacccctgtc gcctgcgatg
    13981 gctggaatcc ttgctccacc aaaagtagcg gtagccagtt gatcaacata taaaccacca
    14041 gcagagtgaa gaaataacac aaccacagca gcagcgtcgc ggttgccgtt tctggcgcaa
    14101 ataaggcacg cagtggtggc gcagcctgtt tttcgccagc gaaaaccgct gactccggca
    14161 gccagcgcac taatagcggc accagaatca acggcaccac accacctacc caaaacaccg
    14221 tttgccatgc taagtttgcc cccgcgaaac ccagtgtcgc cgccagcgcc gcgccaatgg
    14281 gaacaccgca atacatcagg ctcactgccg tcccacgaaa acgtggaccc gcggcttcag
    14341 acgtcagggc gataagattc ggcaacgccg cccccagccc gacaccggtc atcagccgcg
    14401 caaagaccag tgaggggaaa tcccaggcaa tcgccgttgc cagtgagaac aaaccaaaca
    14461 gcgcaactga gccaatcaaa atacgcttac gaccataacg gtctgccagc atcccgccaa
    14521 ccaacgcgcc gggtagcaaa cccagtattc cggcgctgaa tatccagccc atttgcattt
    14581 tatcgagtgc gaaagcctgg gcgatgccac ccgccgcaat gccagccgcc tgaagatcca
    14641 gcccttccat cagagcgacc aaaaaacaaa gcccgatagt cagcatcagg cgggatgaag
    14701 atgatgaagg ggtacgagtc gacataagat gaacctcgtt gcgagtgcag gtgaatcgcg
    14761 actgatacat tgccagccgc gtaatcagaa ctatttaatg actgcctacg ggcgaaatgt
    14821 aggccggaca agacgtttac cgcatccggc agtcgtgtgc agatgcctga tgcgacgcta
    14881 acgcgtctta tcaggcctac aaattctcac gccacccgta ggccggataa ggtgtttacg
    14941 ccgcatccgg cagtcgtgcg cagatgcctg atgcgacgct aacgcgtctt atcaggccta
    15001 caaattctca cgccacccgt aggccggata aggtgtttac gccgcatccg gcagtcgtgc
    15061 tgggatgggc atacaaaatg cacgttctcg gcagtcatca ttatttgttg ttgcgcagat
    15121 ccagcgccac gtcaacgatc atatcctcct ggccgccaac catccggcgt ttgcccagct
    15181 caacgagaat atccaccgca cttaagccat aacgcgccgc cgccgtttca cagtgacgca
    15241 ggaagctcga gtaaacacca gcgtatccca gcgccagcgt ttcgcgatcg actcgtaccg
    15301 gtcgatcctg caacggacgc accaggtcgt cggcggcatc cattaacgca tagagatcgg
    15361 tcccatgctg ccagcccagt ttatccgcgg cggcaataaa cacttccagc ggtgcgttac
    15421 ctgcgcccgc gcccattccc gcgaggctgg cgtcgattcg gtcgcagccc tcttccaccg
    15481 ccgcgataga gttcgccacg ccaagactca ggttatggtg agcgtgcatg ccagtttgcg
    15541 tttctggttt cagctctgct ttcagggcgc ggaaacggtc acggatatcg ctcatgttca
    15601 tcgcaccgcc agaatccacc acataaatac aggtcgcacc gtagccttcc atcagctttg
    15661 cctgcttggc gagattctcc ggcgtggtca tatggctcat catcagaaaa ccaacggtgt
    15721 ccattccgag ctcgcgggca tactgaatat gctgggcgga aacatcagct tcggtacagt
    15781 gcgttgccac acgaaccacc cgcgcgccag cctgccaggc atttttcaga tcgtgaatag
    15841 tgccgattcc tggcagcaac aacgtcgcga ttttggcgtg cttcaccaca tccgccgccg
    15901 cttcaatcca ttcaaggtcg ctatgtgcgc cgaaaccata gttaaagctg gaaccttgca
    15961 aaccgtcgcc gtgggccact tcaatcgaat ccacgcgggc atcgtccagt gctttggcaa
    16021 tctggcgaac gttttccagc gaatactgat gacgaatggc gtgcataccg tcacgcaatg
    16081 tgacgtccga gatataaagt tttttaccgt tcatgccgct tctcctgcct tgcgcgccag
    16141 tgactgggcc attttttccg ctgtcgccag cgcactggaa gtcataatat cgaggttgcc
    16201 cgcataggca ggcagataat gcgctgcgcc ttcgacttcc agccagaccg ctgttttcag
    16261 tccggagaat tgccccacgc ccggtaaatt gaccggttta tcctgcggga taacttcaaa
    16321 ctgcacgcgc tgtttcaggc gataacccgg tacgtaagcc tgcaccgcct cagccatttc
    16381 attgattgag gcttcgatat catcttgtga agcttcgtcg ctcaatacat acaccgtgtc
    16441 acgcatcatc aacggtggct ctgctgggtt aagcacaata atcgccttcc cttttgccgc
    16501 gccgcccacc acttcaatgg ctcgggaagt ggtttccgta aattcatcga tattggcacg
    16561 cgtgccaggt ccggcagatt tactggcgat agaagcgata atttcggcgt aatgaacacg
    16621 cgccacgcgt gaaactgccg ccaccattgg aatggtggcc tggccgccgc aggtgaccat
    16681 gttgacgttc agttgatcga cgttcgcctc gaggttaacc accggcacgc agtaagggcc
    16741 gatggcagca ggcgtcaggt caattaagcg aatatccggt ttcgcttcgc gtaaagcggc
    16801 atcgtttttc acatgcgcgc ccgcgctggt cgcatcaaat acaatgtcga tatcagcaaa
    16861 ttcaggcatg ttcatcagcc cgatcacccc ttcatgggtg gtggcgacgc ccatacgtct
    16921 ggcacgcgcc agaccgtcgg actgaggatc aatgccgacc atcaccgcca tctccagatg
    16981 ctgaccgtga cgcaaaattt taatcatcag atcggtgcca atgttgccag aaccgataat
    17041 ggcgacttta cgcttactca tgacagactt ccttttgggg ctgcgcttga aaatgtcgca
    17101 gcaactgaac ctatgccttc aatatgggct tcaaaacgat cgcccgcatt caccgccacc
    17161 atcgggccta atgccccggt aagaatgata tctccggcgc gcagcggttc acccagactg
    17221 gccattttgc gtgccagcca gacggcggca ttaagcggat gtcccaggca ttcgctgccg
    17281 cgcccgctgg aaacctcttc gttattacgc gtcatcttca tggcgcagtt tttcaggtct
    17341 aaccccgccg gacgttgcgc cggaccgccg atgacataca ccccgcagga agcgttatct
    17401 gccacggtat cgacaaactg aatcgaccag tcgcgaatgc ggctccccac cacctccagc
    17461 gccggaagta cccattcaat ggcgttatac aattcgtcga aggtgatatc agttgcgggc
    17521 aaatcgcggt tcaacaccag tgcgatctcc gcttcaatgc ggggttgcag aacacgggaa
    17581 aaaggaatga tttcgttatc gccataacac atgtcggcaa ataacgtccc aaaatccggt
    17641 tgatcaacgc ccagttgttg ttgcactttc ggatgtgtca ggcccacttt acgccctacc
    17701 acgcgacgcc cctgcgcaac gtcatgttgc acatttatgt gctgaatggc gtaagccgct
    17761 tcagcgttat cgataccaat cagatcgcgc agcggtgcaa tcgcttcgcc ctgctctgcg
    17821 gcgcggcgta aatccgccgc cagttgctca agagtatgct tcgtcatgac cattccttaa
    17881 gggcgtgcga ggaaattcag caccagttga ttgaaagcgt cggcatgttc ccactgcgcc
    17941 cagtgaccgc agtcgcggaa gatatgcagt tcagaaccgg caatgccgga cagcagacgc
    18001 agacccgcat ccatcggcac aaagcggtcg ttgcgccccc agacaatcag ggtttgcgct
    18061 ttgatttccg ccagacgtgg gccaaaatcc gggaactgtt tcggattagc ttccaggctc
    18121 ttaacgaagt tttccaggtg atcgcggcgc gacagcatat tattcaggcg cgcttcaaac
    18181 agggcgtcgg tcaaatcgct ggtatcaaaa acgaagatat ccatcatcag tttcaggttt
    18241 tcgatagtcg gctgacgata aagctgattc agtcgcttaa taccttcggt tggcatcggc
    18301 gtaaacaaac tcatgccgcc cgtaccgccg cccatcagca ccagtttgcc gacgcgctcc
    18361 ggccagttaa gggtgaacgc cacagaacta tggccgccca tcgagttgcc caacaggtgg
    18421 attttggcga tatccagttg atccaccacg cttttcagga ttcgtgcatt aagatccgat
    18481 cgtgaaccac tattaacgat cgaatcgctc ttgccccaac ccggacaatc cagcaggatc
    18541 acccgatagc ccgcctctac cagcggatcg atattgcggc tgaaattcgc ccagccagta
    18601 gcacccgggc cggaaccatg cagcaggaca acggtttcgt cgccttgtcc gcagtcatta
    18661 aaatggatgc gcagcgtttt acccgcttct tctacattca gaaaacggct ggtggcggct
    18721 tcggtttgtg gctgataact catcatcttc tcctgcatat tcagttctct gttctggcgc
    18781 ttaacgagcc aaatccggca atccactccg ggattgggcg gtaataacgc ccttcgctac
    18841 gccagttgcc aaatgcagaa atagcggcaa aagcggcgac ccaggttttg atttcatgtg
    18901 tcgactttcc ggcaatggcg gaaagctctt cgttactgac ggcatccagt tcctgtatgc
    18961 gtccctgctc cagcaaagtc atgaactggt tatcccaaat cgggttgagc ggatgcagag
    19021 ttctctgatc ctcaacaaac ttctcagcgg cgctaatcac ccgttgctga cgcaattcgc
    19081 gctcactggc gggtaaatct ttcccgctcc ccaacagacg gtcgcgcata tgggcatcgg
    19141 ctttcgccag ttcgggcacc ggcggctgat gggaaagccc accggaaccc aggaacagca
    19201 cgcgtttatt gagagtgctg gtgaaacgtc caatggcttc acccaacatg cgggtacgct
    19261 ggaaaccggg cagcggcgtg gcgacaccgt tgatgaacac aggcagaact ggcaccttat
    19321 ccagcccgcc gagcaggaac tccagtggct gggcgaaccc gtggtccacc tgcatacagt
    19381 aagaaacggc aagatcgatc ccgctcttca tgacggcatg cgcacaggcc tccgccagct
    19441 ccacaggcac gggcagctct cctgccgcac tgccgaaatc accaattgcc gtcgctccaa
    19501 cgcctaaaca gaacggtggc atcacgtcat agaaaaagcc gttgtagtga tctggcgcaa
    19561 acagcaccac cagttcaggg gagaaagccg caatacgctc gcgggcgctg gcaatcacgc
    19621 cattgacctc atcgagtacc tcttgtgccg ggtcgacata ccccaccagc ggcgagtggg
    19681 aaagacaatg aagataagcg tgcatatcag gctacctttt cgacagaaac gtcggcatca
    19741 gggcgggtca gcgtcatcac cgacgccagt ttattcaggg tcttgcccag ggtttgcgga
    19801 atggcggtgg cggcaacaaa gcgatccggg cgcatcacca ccagcgaagc attgtgttgt
    19861 gcgaaccagc tacgcagacg accttgcgta tcgcccacgc gtagcacgcc gtcgtggtta
    19921 tcctgtgccg tatgaatttg cacttccggc accacctgaa taaagcgtgt gcccaacgcg
    19981 cgccactgct ggatttgctc gtcgctcatc ccccacagtg gattgcatcc ccagccaatt
    20041 accgcgaagt tcgcgccgat cgcgttatcg agcagcgtca cgtcgccgtt ttccagcgtg
    20101 actttcggct gaataaacat cttgccgacc ggagagtgct tcgcttcgcc ctcacgtacc
    20161 agcgcaccgc cgtaatattg cggcatcggc ttgaagcgca tttcgaggaa gtagcgtttt
    20221 actggcggca gataattcag cagccaggaa acgccgtcac gtaacgtacc ctgccagcgt
    20281 ttcggtggag ccagcacgtt gcccgccgtc acggacaggt caatcatcgc tttggcgtga
    20341 tcgcgacgtt cttgttgata ggtattgagc agcgcatcgc gggctttccc ctggataacc
    20401 aacgccagtt tccatgcgag gttaaaggcg tcgcgcatac cactgttata gccctgcccc
    20461 tgccacaccg gcatgatgtg cgcggcatcg cccgccagca gtacgcgatc aatacggaaa
    20521 cgttgcgcca gtcgcgcgtt gtgggtgtag acacgctggc gaatcaattc aacattgtcc
    20581 ggattaggca gcactttgct taacagcttg cgcatatttt gcggctcacg cagctgttct
    20641 tcggtttctc ctggcatcac cataaattca aagcgacgta ccgcatgagg tagcgcggca
    20701 gaaacatacg ggcgcaccgg atcgcaacac aaatagatat gcggcgtact taacggatcg
    20761 ttggcgatat ctaccacaat ccactgattt ggcgcagttt taccttcaaa cggcacattc
    20821 agggtgcgac ggacaaaact tgccccacca tcacaggcca ccagccactg ggctttgact
    20881 atttcccgct gcccttctgc cgttttcagg tgcaaggtca cttcgtcatc ttgctgactg
    20941 aaggcctcca gctcgcggga aaacaagcag cgcacattcg gaaaacgcga caccccttcc
    21001 agcatcaccg catcgacctg cggctgaata aaggcgttac ggcgcggcca gccaaattca
    21061 tcggtcattg gctgaatatc agcaaaacag cggcctttcg gggtgagaaa acgcatcgcg
    21121 tgccacggcg tagtgtgcgg cagaacatca tcgaccaggc cgaccgactg catggtgcgc
    21181 agcgcctcgt catcaatacc aatcgcacgc gggtagtcga tcaacttatc gagtttctcc
    21241 accaccagca cgtcaatgcc catctggccg agatagttcg ccatcatcag cccaaccggg
    21301 ccggcaccag cgatcgccac ctgaacgcta tggttaacag caggctggat gtcagggtgt
    21361 tgtattgcca tttcagtacc tcacgactcg gacaaaatgt cgttgcgcgc acagtacagc
    21421 gcaacttatt ttgttaaaaa catgtaaatg attttttatt gtgcgctcag tataggaagg
    21481 gtgttttcgg ctacaatcaa aacatgcccg aatgtgcacc aggtgcacca cgttgtttta
    21541 actatagaaa tgtcaattaa tatgcagaac aatgagcaga cggaatacaa aaccgtgcgc
    21601 ggcttaaccc gcggtctaat gttattaaat atgttaaata aacttgatgg cggtgccagc
    21661 gtcgggctgc tggcggaact cagcggcctg catcgcacca ctgtgcggcg actgctggag
    21721 acgctgcagg aagagggata tgtccgccgt agcccctccg atgatagttt tcgactgacc
    21781 atcaaagtgc ggcaattaag cgaaggattt cgtgacgaac agtggatttc tgcactggcg
    21841 gccccactgc tgggcgatct gttgcgcgaa gtggtatggc cgacagatgt gtccacgctg
    21901 gatgttgatg caatggtggt acgcgaaacc actcaccgtt tcagccgctt atcctttcac
    21961 cgggcaatgg tcgggcgacg tttgccgctt ctgaaaaccg cctcgggcct gacctggttg
    22021 gccttttgcc cggaacaaga ccgcaaggaa ttaatcgaaa tgttagcctc ccgccccggt
    22081 gatgactatc aactggcacg ggaaccgtta aagctggaag ccattctggc gcgcgcgcgc
    22141 aaagagggtt acggacagaa ctaccgcggc tgggatcagg aggagaagat cgcctctatc
    22201 gccgtaccgc tgcgcagtga acaacgggtg attggctgtc tgaatctggt gtatatggcg
    22261 agcgcaatga ccattgaaca ggcagcggaa aagcatcttc cggcgctaca acgggtagca
    22321 aaacagatcg aagaaggggt tgaatcgcag gctattctgg tggccggaag gcgaagcggc
    22381 atgcatttac gttgacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg
    22441 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc
    22501 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
    22561 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
    22621 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
    22681 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
    22741 gggtgcctgc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
    22801 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
    22861 ccaggatgcc actgctgtgg aagcagcctg cgctaatgtt ccggcgttat ttcttgatgt
    22921 ctctgaccag actcccatca acagtattat tttctcccat gaagacggta cgcgactggg
    22981 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gtccattaag
    23041 ttctgtctcg gcgcgtctgc gtctggcggg ctggcataaa tatctcacac gcaatcaaat
    23101 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
    23161 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
    23221 gctgggcgca atgcgcgc
//
LOCUS       DAOFQD010000071        23009 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.071, whole genome shotgun sequence.
ACCESSION   DAOFQD010000071 DAOFQD010000000
VERSION     DAOFQD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.071"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            107..778
                     /locus_tag="QBF46_002864"
     CDS             107..778
                     /locus_tag="QBF46_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675025.1"
                     /translation="MKMLVEQAEANISRALIDADKPEAVESGEFPDEQHHEDGRITTL
                     PSTYYSCGSCFGYDEDEVRSEYKHLIVQLTRRSVFLTIFGLFEHRMVDCLALMDDLSS
                     KTTDKTRKTIEDCHKRLKRNFGGKSIKDVDHLAVIRNIMAHSDGVAEDYKTLSCKKTK
                     KTEYEKPFLRAIPRAMAENAGVSINMFNDILMDDRFLMYAVGEFERYVNELNTAVRTY
                     QSRLT"
     gene            complement(1170..2669)
                     /locus_tag="QBF46_002865"
     CDS             complement(1170..2669)
                     /locus_tag="QBF46_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDQ6675026.1"
                     /translation="MANIITCKTKDGKTIQYVDEVIGSGSMKDVYFSPDKSYVVAFYH
                     KPQNEQARERINMITGRYRQNIFEQTGGDYWKGLFCWPTDVVEHADKVGIVVPAYQQH
                     FFFRYGSKNNDFLGIKGREKEGKWFASANNQNKFLDPRERGNTLNYLKVCILLTRAVR
                     RMHAAGLCHSDLSYKNVLIDPELGHACIIDVDGLVVPGKFPPDVVGTPDFIAPEVVKT
                     SHLPKDDPRRVLPSIATDRHALSVLIYMYLLFRHPLRGGKIHDIDDEVRDEALSMGER
                     ALFIEHPTDRSNAVKVNQVSSFSLPWADPQKIPYTIMGPYLKPLFDRAFIDGLHDPSK
                     RPTADEWESALVKTVDLIQPCQNNDCDQKWYVFNGKTKPVCPYCGTPYKGKLPILNLY
                     SSRKAGSFRPDDHRLMVWSGQSLYAWHVNRLIAPNERTTDEQKKRVGYFVFHNDQWWL
                     VNEGLSGLISLPDRKTIGIGEKLLLEDNTQFILSSEDGGRLVVVQLLNN"
     gene            complement(2697..4430)
                     /locus_tag="QBF46_002866"
     CDS             complement(2697..4430)
                     /locus_tag="QBF46_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDQ6675027.1"
                     /translation="MSEFITPEHKIIALILEASGEPTDADKVFCLGNIPDVALAVRQL
                     REMVIQHSASEAIPAENTQTVSERLTQPFSDNILPPQNAGESEADRPEIEPVHAATAL
                     QGNDKQEAHSEMVDDKVSEGVQGHTEEVPDETGVLQTEASVTVQPEDMDKTSPVVSDN
                     LSPDTAVSALRPGEVPQRVENKTSPAPGKVPEVFIILPNARAGEPYDVRLEIATDTGV
                     EAEITEVNFSQDIGLIFDINERRLQGTPGHSGDFELTVKWSCSSHRDNEKKLLFVVNP
                     DPKSLWKVIDPPSDAPFYKKSIDHQSVCRSGIHIAGASRRGRSHEHVGSFRDDDYYLN
                     FSDVSGWSVLLVADGAGSAKYSREGSRIVSETVGNYLFNQFKTEKGLEIKNRVLRWNS
                     EDQRAVWEFMNHHFRQAAVLAVNNISSAAILAEDKVKSFSTTLLATVTFRDGADLFAA
                     SFWLGDGAIAAYGPAGKVRVLGTPDSGEYAGQTRFLDADAVSDAGFSKRISIGKWNDI
                     SHLVLMTDGVSDPWFETDNGLQNPQKWDRLMAELSPLLTDPEHASAQLVEWLNFFSPG
                     NHDDRTIIVLW"
     gene            complement(4430..5470)
                     /locus_tag="QBF46_002867"
     CDS             complement(4430..5470)
                     /locus_tag="QBF46_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ6675028.1"
                     /translation="MRRLPVFFVLDCSESMIGENLKRMNDGLQKIVGDLRKDPHALET
                     VWVSVIAFAGVARTIVPLHDIVSFYPPRLPVGGGTNLATALRELTTQIDSQVRKTTQE
                     EKGDWKPVVYLLTDGRPTDDISAEVKRWNEFYAKKLNMIAVGIGSSVDLSVLRQLTEN
                     VLSFTENQEGDFSRFIQWITASVTAHSRSVGEDSQPPLKQTDYIVKLAKDDTARAYDE
                     NCVVLTGRCSKTRRPYLIKYERPPARVSGLAFDLNLNSFNIAGCYPVDEDYFSWTDMA
                     VAGAQINTSELHGVPGCPHCGNSSAFAMCTCGKLLCIDGPDDVNCPWCGKGLSFQSEG
                     GNSDFNVSRGRG"
     gene            complement(5563..6201)
                     /locus_tag="QBF46_002868"
     CDS             complement(5563..6201)
                     /locus_tag="QBF46_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ6675029.1"
                     /translation="MRRLPVYLLIDTSGSMRGESIHSVNVGVQAMLSALRQDPYALES
                     VHISIITYDNEAREYVPLTALADFQFSDIEVPSAGGTFTGAALECLIQCVDRDIRRSD
                     GEQKGDWRPLVFLMTDGTPSDAWAYGEAVKEVQRRSFGSIIACAVGPKAGHEHLKLLT
                     DKVVALETLDSTAFSGFFKWVSASVASGSSSAGVTTDSDTLPPPPPEIQLVL"
     gene            complement(6202..6843)
                     /locus_tag="QBF46_002869"
     CDS             complement(6202..6843)
                     /locus_tag="QBF46_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDQ6675030.1"
                     /translation="MTVSLRKGQGVSLRKNEFDLSSVTIGLGWDINEEKKGFLGGIFG
                     KKEEEYDLDVIAFLCNAAGKVTDLGNVENGKPTLVNGDIVFFNSLRHKSGQIWLTGDN
                     RTGAGDGDDEQIIVKLNSIDPKYEKIVFIVQIYNGEELQQHFGKVQNAFIRAVDGKNV
                     EMARFDLSGGVAFNGQRSMVFAELVRESSGWKLNAIGEPSTSDSFVSHLRNYI"
     gene            complement(6868..7506)
                     /locus_tag="QBF46_002870"
     CDS             complement(6868..7506)
                     /locus_tag="QBF46_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ6675031.1"
                     /translation="MRRLPVYLLLDTSGSMHGEPIEAVKNGVQTLLTTLKQDPYALET
                     AYVSVITFDSTARQTVPLTDLLNFNLPSFSASGTTALGEALSLTANRIDAEVQKTTAE
                     TKGDWRPLIFLMTDGGPTDDWRKGLNEFKAAKKGVVVACAAGHDADTGVLKEITEIVL
                     QLDTADSSSIKAFFKWVSASVSVGSQKVDAGKKEVIGLDDLPPPPPEVNVVL"
     gene            8312..8506
                     /locus_tag="QBF46_002871"
     CDS             8312..8506
                     /locus_tag="QBF46_002871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675032.1"
                     /translation="MANLKTKNSIRRQLRWLRNLRDYYLDDREFVRILSNEIRVLEKL
                     LYDIVMEEYNPLTTRFDKWD"
     gene            complement(8769..9227)
                     /locus_tag="QBF46_002872"
     CDS             complement(8769..9227)
                     /locus_tag="QBF46_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HDQ6675033.1"
                     /translation="MQLNTRQERVYTLANILGSGKPVSAQSITATLACSEPTLSRALK
                     ELRETYAAEIKYSKAGHSYQMVNSGLLDKKTLKRMSEALDLNTDLKTHDQGARVILDK
                     DIKTSVSLSIRRRILRKIDKLAELSGTSRSEAVEKLVEMKIDEIIKVLKK"
     gene            complement(9230..10453)
                     /locus_tag="QBF46_002873"
     CDS             complement(9230..10453)
                     /locus_tag="QBF46_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDQ6675034.1"
                     /translation="MGFRFRKSISIIPGVRVNLSNGAPSLSIGPRGASLSVGKNGTFA
                     NLGLPGTGLSYRTRIDRTARERVNTRHQADPGLKSELELVVEKLMSTVTVITNIHELS
                     PSPKGGNTWAMLETQYLALRQGSFSLPAPVRPNKPEYIPLPPEPDEHAGRGFLGGWFE
                     SDAARQERQNENLRRWQVTVADIERENELLKQRYEKQRIAWAEQYAEWQHQYQEHEKN
                     RTESVALAKEQFRSDARFFEHCLEEFFSQTEWPRETLVSFEVRPEESTVWLDVDLPEI
                     EDMPDKVYSVNARGTDINEKAMTQKAARESYAKHVHGCLLRLAAIVFQTLPFEQVVIS
                     GFTQRVSKRTGYLEDEYIISWRVCRSEIELINFGNLRGLDPIEALGDRGLIRKMSATY
                     IFQPIEPLTHMAGTD"
     gene            complement(10464..11420)
                     /locus_tag="QBF46_002874"
     CDS             complement(10464..11420)
                     /locus_tag="QBF46_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDQ6675035.1"
                     /translation="MKKQPNPWELGATLYMPATRKDIAEVVLEGKIPGLRSLVVCLED
                     AVSEHDIPLAIQNLSLFLERLRHARAVNDDEKYPLVFIRPRHPDMGRWLTANLDLSAV
                     DGFVLPKFTRSTLPVWWDIMAGTSLMMMPTLETEEVYDVIKMQALANELSSHDCRDRI
                     IALRIGGNDLMNVVSLRRPRHLTLYDTPMGYVIKMLVSVFAPRDFALTAPVCEHIDDH
                     GVLGRELSMDIAHGLVGKTAIHPDQISVIEEALKVSPGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAQGILGRGQVYGLTTGQNPEGIRLIPVSKQH"
     gene            complement(11420..12499)
                     /locus_tag="QBF46_002875"
     CDS             complement(11420..12499)
                     /locus_tag="QBF46_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDQ6675036.1"
                     /translation="MSHFAPFSGSYKPDDVHFLLKPIVMEMTPVDLKEELIQSGKMHY
                     SDMLSQEPEPTRWHLDLFTRALDSGAARLAREVSYLARELARRAGDEPIVLVSLVRAG
                     VPLGVMLHQALRAMGKQSFHYGISIIRDRGIDAAALDFIESRHGTGGIVFVDGWTGKG
                     AITGELSRTLKDRPGYPEQPRLVVLADPCGCAWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWAEEGLHGCVVCDHLQSFECSQMLVDTVAHFREQLDGKPLPASGWDAGKNAARWQL
                     SRKVIQSLAARYEVDSINRIKPGIAEATRAVLRRVPDHVFVRQMDDPDVSLLVGLAKE
                     KGIQVTEAGDTLGQYRAVTIIKKVR"
     gene            complement(12501..13274)
                     /locus_tag="QBF46_002876"
     CDS             complement(12501..13274)
                     /locus_tag="QBF46_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675037.1"
                     /translation="MNKPVVFSDLDDTLFQTRRKMVDELALEPFRTGALDRSLTPRSF
                     MTEEQAMLVDWLLEHADLIPVTARGTEEISRVQIPFRSWAVTTHGAVILRPDGTPDSD
                     WKAHMLECLSSYADRLTSMQHIITELMDARGINAWARLNYEYEGTPVYLVMKHRDSTK
                     LDELNAVGDEIERVFSTEGFYIHRNSNNIAWLPCPVEKGLAVSWLLEKLRQERGVFPV
                     MGLGDSLSDHRFMKLCSWFGLPRQSQFAEAISQRIFGEQ"
     gene            complement(13267..14409)
                     /locus_tag="QBF46_002877"
     CDS             complement(13267..14409)
                     /locus_tag="QBF46_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HDQ6675038.1"
                     /translation="MSAIDGNFVYQKTLSCGRIEVSSEVTDYPLTDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVSPQVMRNVYTQLAEKFPGTLDGPVVFIGMAETAVGLGAGVFDE
                     VRKHQPDCVYLTSTRHPVDAELLCEFKEEHSHATDHLIYYPVCAGMRQTVAEAQTLVL
                     VDDEATTGKTFLNLLAALRASGALRKVRRVIAVTLTDWSGEALYQNCPLPLHTVSLIS
                     GSWRWTPEPDAPLPVMPQVNVTSRGNIPITGKQSWGRLGMQIPASDLGLQVQVSPGEN
                     ILVLGTGEFVWEPFLLAERLETAGAQVVFSSTTRSPISTGYAIQSAISFSDNYGLGIP
                     NYVYNVAHQQFDRILICCETPVASVDPRLLEALSAVAPTLEVITYE"
     gene            complement(14421..15479)
                     /locus_tag="QBF46_002878"
     CDS             complement(14421..15479)
                     /locus_tag="QBF46_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDQ6675039.1"
                     /translation="MTKKIWFMEGLSSQRDIVLGVKSFAEKENQNIVFFSSHRNERNE
                     ILSVSDFSMLEPKSEEERLSFIHSITSAHGINAIHTGRNCKWFESHRENIQRSGVHLT
                     TGSTGTHWLELADEKVTFSEFMEKNGLPVVPSVRVKNVDELRALLSDPPFPSDCLCIK
                     PVTGIYGMGFWRFDDNVSPVAFLNNPDNRVIHPSQYLAAHARMDCFTPQVLMPYLPGP
                     EYSVDMVVSQGTVLAAIGRRKVGVLQYLENEGEAIELAIDCARLMNADGMVNVQTRHN
                     AEGKPLLLEINMRPSGGIGYTRHSGVNLPGLYAFHTLGLMDDEQVRQSAKTDFSPAVV
                     RSVTDVIPYPASLTNRID"
     gene            15790..16374
                     /locus_tag="QBF46_002879"
     CDS             15790..16374
                     /locus_tag="QBF46_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance TerZ family protein"
                     /protein_id="HDQ6675040.1"
                     /translation="MVSLSKNQTVSLSKQSTDLSQLQFGLGWDPVKKKGLFGSFFGGN
                     DSIDLDAGCVLLDVAGNKIDTIWFRKLKSSCGSVVHSGDNLTGEGDGDDEIIHVNLLK
                     LPANVEYLAFTVNSFRGQSFNDVDNAFCRVVDQTNKELARYQLTEQGPHTGIIIASLR
                     RNNGHWDFTAHGKASGGRTIDDMQRDIISTVVKA"
     gene            16371..17522
                     /gene="terA"
                     /locus_tag="QBF46_002880"
     CDS             16371..17522
                     /gene="terA"
                     /locus_tag="QBF46_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance system protein TerA"
                     /protein_id="HDQ6675041.1"
                     /translation="MNLTPGGNAPVPAQELRVRVISGGPVDASAFRLFADGKVRGDSD
                     MVFYGQPRNEDGSISFSTEGTNSVFTVDLSRLKPDVQKVAFTVTCDGSHTVSSLNHLS
                     IQIESGNTSLISGQVELSGRQEAALILGEVYRRNGDWKFRFIAQGFNGGLKPLAEYYG
                     VNVADEPAPVTPPPAPPAPPKVSLSKVSLTKEKPAISLAKKDNFGEIRINLNWHRGSG
                     SGGLLGGMFGSNKAIDLDLGAFVELADGYKSVVQALGNAFGNYHSEPYVQLQGDDRTG
                     EALDGEWLHINGREWKEIRQVLIYAFIYQGVPSWDKTDGVVTLHVPEQPPIESRLTEG
                     DNKRTLCAIARLVNENGSIRVERINQYFRGQEEMDRAFGWGFRWSRGSK"
     gene            17545..18000
                     /gene="terB"
                     /locus_tag="QBF46_002881"
     CDS             17545..18000
                     /gene="terB"
                     /locus_tag="QBF46_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerB"
                     /protein_id="HDQ6675042.1"
                     /translation="MSFFDKVKGALTSGREELTRQVGRYKNKKFMQGTVAVCARIAVA
                     SDGVSSEEKQKMIGFLRSSEELKVFDTAEVIEFFNKLVTSFDFDLEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDDDEKSAVREIARSLGFDPAEFGL"
     gene            18024..19064
                     /gene="terC"
                     /locus_tag="QBF46_002882"
     CDS             18024..19064
                     /gene="terC"
                     /locus_tag="QBF46_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HDQ6675043.1"
                     /translation="MVSTHIGFPAETVTVFVALSVGAIFIDLFMHRHDKPISLKSAAL
                     WSVFWVVVAMAFAGFLFVHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFSVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTGLLSLGPYVELIFAVVVAWTAVMMLKGGGDDDE
                     IEDYSQHLAYRLVKRFFPIWPKLSGHAFLLTQKEVDAELAKPENKDVSVGRLKKAALY
                     ATPLMLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLSQLEKAVIVLLFFIAFKLGLNATDHIWHHGYSLSATTSLYVVLGVLALGILASVI
                     FPEKPEDENKES"
     gene            19103..19681
                     /gene="terD"
                     /locus_tag="QBF46_002883"
     CDS             19103..19681
                     /gene="terD"
                     /locus_tag="QBF46_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HDQ6675044.1"
                     /translation="MSVSLSKGGNVSLSKTAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLAANGKVRGDADFIFYNNLKSADGSVTHTGDNRTGEGDGDDESLKIKLDAVPGDV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            19768..20343
                     /gene="terE"
                     /locus_tag="QBF46_002884"
     CDS             19768..20343
                     /gene="terE"
                     /locus_tag="QBF46_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance cAMP binding protein TerE"
                     /protein_id="HDQ6675045.1"
                     /translation="MAVSLVKGGNVSLTKEAPSMNVALVGLGWDARVTDGQAFDLDAS
                     VFLVGENGKVLSDSHFVFYNNTTSPDGAVQHQGDNRTGEGDGDDEQVKIDLTKVAADV
                     KKLVFAVTIHEADSRKQNFGMVSNSFMRVVNNDNGAEIARFDLSEDASTETAMIFGEL
                     YRHGAEWKFKAVGQGFAGGLAALATQHGINI"
     gene            20428..21669
                     /locus_tag="QBF46_002885"
     CDS             20428..21669
                     /locus_tag="QBF46_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ6675046.1"
                     /translation="MQLQSGQNIPLHTDTLTLRLDYPPSPGFHSTPETCLFMLNAEGK
                     VTGDADFIFYNNLSAAGGSVKLNPGHQHAVVHFALDQIPDTIQKIAITMVIDGSDTIS
                     GLAQLKLSAESQATFFVDLNGRTEKAVIMGEVYRYQGNWKLRALGQGFNGGLEPLAVS
                     YGVDVEPAAEVVPQPARISLEKKLEGKAPALISLAKKASISLAKHKLDTVEARVAFVL
                     DASGSMTGQFKKGHVQAVLDRIAVLSVQFDDDGTMDVWGFAERHKKYPDVTLDNLDGY
                     IAAIQNTGKRSAWEILPGLGGTNNEPPVMNEVIDYFKDSTLPVFVVCITDGGISKTRE
                     IKEAIRRSANYPVFWKFVGLGGSNYGILERLDTFSDRRVDNSNFFAIDNFAHIKDEEL
                     YEKLLEEFKDWLGLAKKEGII"
     gene            complement(22006..22653)
                     /locus_tag="QBF46_002886"
     CDS             complement(22006..22653)
                     /locus_tag="QBF46_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDQ6675047.1"
                     /translation="MNVIQPRMLSVFDFDGTLTRHDSFIPFLRFAFGNRAFSRRIVSM
                     ALPTLRCMGKQMTRDELKETLITTFLTGVEEKWVSSKAEEFCQLYWHRLMRPAGLIAV
                     ANEVTSGAIVTLCSASPEIVLRPFAEKLRIKLIGTQLETKDGILTGRISGHNCRCSQK
                     IIRLEKEYGPLTDYHLRAWGDTRGDYELLSAATEPHWRHFHQGFRRKLPSKLIIR"
BASE COUNT         5916 a         5648 c         5457 g         5988 t
ORIGIN      
        1 aaagcgtgcg tattgcctga aaatccagcc agctacaggg tcgttcgcac gaaatcttat
       61 ttattcaaca aagccgttac aggaagcaat ctcttcaata cctgtcatga agatgcttgt
      121 tgagcaggca gaagcgaata tatcccgtgc actaattgat gctgataagc cagaagccgt
      181 tgagtctggg gaatttccgg atgaacaaca tcatgaagat ggtcgaatta caacgcttcc
      241 aagtacttac tattcatgcg gttcgtgctt tggctatgat gaagatgaag ttaggtctga
      301 atacaaacat ctgatcgtgc agctgacccg ccgctccgta tttcttacca tttttggtct
      361 gttcgaacac cgtatggttg attgcctcgc actaatggac gacctttcaa gcaagaccac
      421 agacaaaacg cgcaagacca ttgaagattg ccacaagcgt ctcaagcgca acttcggcgg
      481 taaaagcatc aaggatgtcg atcacctagc tgttatccga aacatcatgg ctcatagcga
      541 tggtgttgca gaagattata agactctttc ctgtaaaaaa acaaaaaaaa ctgaatatga
      601 aaaacctttc ctgcgtgcta ttcctcgtgc gatggctgag aacgccggtg tgtcaattaa
      661 catgtttaat gacatactta tggatgatcg gtttctgatg tatgccgtcg gtgaatttga
      721 gcgctatgtt aatgaactga acactgcagt tcgcacttat caaagcaggt tgacctgaag
      781 gaaccgtctg cttatacgct cagttatgag gtaaagtaag caattcacag tctggatatt
      841 catttaacgg agttattata tataagttgc agactactcg ctaaatttgg tgataaaggt
      901 gcaattaact caggtaaaac gatccctcgt gtaggctgag ttattggtga ggcttttgat
      961 ggagtttcaa ccaatcttgt tggtaatata gcgaggaagc ccttcattga aggaaatata
     1021 atcaatgatg tctaaagatg gtctgagtat gtcgaatact gaattgaaaa attagtatac
     1081 ccgtaatgtt gcgtttcgat tgtgataatt atggcccacc taatggtggg ctcatattga
     1141 cgttatgata tcaatggcca gtaatatatc taattattca aaagctgaac caccaccaat
     1201 cgcccaccat cctctgaaga caggatgaac tgggtgttgt cctccagtag caacttttca
     1261 ccaatgccga tagttttccg atcgggaagt gatatgagcc cgctaagccc ctcgttaacc
     1321 agccaccact ggtcattatg gaagacgaaa taaccaaccc ttttcttctg ctcatcggtg
     1381 gttcgttcgt taggagcaat cagtcggttg acgtgccatg catataatga ctgaccgctc
     1441 cacaccataa gccggtgatc atctggcctg aaacttccag cttttctcga cgaatacaga
     1501 ttcagaatgg ggagtttgcc tttgtacggt gtaccgcaat agggacaaac gggtttggtc
     1561 ttgccattaa aaacgtacca tttctggtca caatcattgt tctggcatgg ctggatgaga
     1621 tcgacggttt ttacaagggc gctctcccat tcatcagctg tcgggcgctt cgacggatca
     1681 tgcaacccgt caataaaagc ccggtcgaag agaggtttca ggtagggccc cataatggtg
     1741 tacgggattt tctgaggatc tgcccacggg agcgaaaacg atgagacctg attaacttta
     1801 actgcattgc ttctgtcagt cgggtgttcg ataaaaagtg cccgctctcc catggaaagc
     1861 gcctcatccc tgacctcatc atcgatatcg tgaatttttc ctccccgtaa cgggtgccgg
     1921 aaaagaagat acatgtaaat gagtaccgaa agtgcatgcc ggtcggtagc gatactcggc
     1981 aatacgcggc ggggatcgtc tttggggaga tggcttgtct tcaccacttc cggggcaata
     2041 aaatcaggtg taccaaccac gtctggagga aattttcccg gaaccaccag cccatcaaca
     2101 tcaataatgc atgcgtggcc tagttccgga tctatcagca catttttata actcaggtca
     2161 ctatgacaca ggcccgcagc atgcattcgt ctgactgcac gcgttagaag gatgcagacc
     2221 ttcagatagt taagcgtatt gccacgttca cgcggatcca gaaatttatt ctgattgttc
     2281 gcgctggcaa accatttccc ttccttttca cgtcctttga tcccgaggaa atcattgttt
     2341 ttggaaccat atcggaagaa gaaatgctgc tggtaagcag gtacaacaat acccacctta
     2401 tcagcgtgct caaccacatc agtgggccag cagaagagac ctttccagta atctccaccc
     2461 gtctgctcaa aaatattctg ccggtacctc ccggtgatca tgttgatacg ttcccgggcc
     2521 tgttcattct gaggtttatg gtagaaggct acgacatatg atttatccgg agaaaaataa
     2581 acgtctttca ttgagccaga gccgatgacc tcatcgacat actgaatggt ttttccatcc
     2641 ttagttttgc atgtaattat atttgccatg aatagccacc tgctatcgca ttaatttcac
     2701 cagagcacaa tgattgtgcg atcatcgtga tttcccggag agaaaaagtt aagccactcc
     2761 accagttgtg ccgaggcatg ttcagggtct gtgagtaggg gagagagctc ggccatcagc
     2821 ctgtcccatt tttgtggatt ctgaagcccg ttatccgtct caaaccaggg atcggaaaca
     2881 ccatcagtca tcagaaccag atgcgatatg tcgttccact taccgatact gatacgttta
     2941 ctgaatccgg catcactgac ggcatcggca tccagaaacc gcgtctgacc tgcatactct
     3001 ccgctatccg gtgtcccaag cactctgact ttgccagcag gtccatatgc agctatcgca
     3061 ccgtcaccta accagaaaga cgcggcaaac agatcggcac cgtcgcgaaa agtgacggtc
     3121 gcgagtaaag tcgttgagaa tgactttact ttatcttcag ccaatattgc ggcactgctg
     3181 atattgttta cggccagtac cgctgcctgt cggaagtggt ggttcataaa ctcccatact
     3241 gctcgctgat cctcactgtt ccagcgaagg accctgtttt tgatctcaag acctttttca
     3301 gtcttgaact ggttgaagag atagttacca acagtttcag aaacgatacg ggagccttcc
     3361 cgggaatatt tagcgctccc cgcgccatca gctaccagta atacactcca tccggataca
     3421 tcactgaagt tgaggtaata atcgtcgtca cgaaaagagc ctacatgttc atgggagcgt
     3481 ccacgacggc ttgctccggc aatatgaata cctgaccgac agacactctg gtggtcgata
     3541 ctcttcttgt agaatggtgc atcagaaggc gggtcaatca ctttccagag acttttcgga
     3601 tcagggttca cgacaaaaag taactttttt tcattatcac gatgtgagct acatgaccat
     3661 ttcacagtca gttcaaagtc tccgctgtgc cctggcgtcc cctggaggcg tcgctcattg
     3721 atatcaaata tcagccctat gtcctgagag aagttaacct ccgtaatctc tgcctcaaca
     3781 cctgtatcag tcgctatttc cagtcgaaca tcgtagggtt cacctgcccg tgcatttggc
     3841 agaatgataa acacctccgg cacttttccg ggggccgggg atgttttatt ctccactctc
     3901 tgaggtactt ccccgggcct taaagcactg actgcggtgt caggtgaaag gttgtcagaa
     3961 acaaccggac tggttttatc catgtcttct ggttgcaccg tgacggaagc ctcagtctgt
     4021 aagaccccgg tttcatcagg aacttcttca gtgtgtccct gaacgccttc tgacacttta
     4081 tcatccacca tttcactatg cgcttcctgc ttatcatttc cctgaagtgc agtagcggca
     4141 tggacgggtt caatctcagg tcgatcagct tcgctttcac cagcgttttg aggtgggagg
     4201 atattgtcag aaaagggctg ggtaagacgt tctgaaactg tttgagtgtt ctccgccggt
     4261 atggcttcgc ttgcagagtg ttgaatgacc atttctctca gctgccgcac agcaagggca
     4321 acatcaggta tgttaccaag acagaatact ttatctgcat cagtcggttc ccctgatgcc
     4381 tccagaatga gagcaataat tttatgttca ggtgtaatga actcgctcat tagccgcgtc
     4441 ctctgctaac gttaaaatct gaatttccac cctcgctctg aaaggacaaa cctttcccac
     4501 accatgggca gttcacatca tcaggcccat caatacagag aagttttccg caggtacaca
     4561 tcgcaaaagc actactgttt ccgcagtgtg ggcaccctgg tacgccatgg agttcactgg
     4621 tgtttatctg cgcccccgct acggccatat ccgtccatga gaagtaatct tcatccacag
     4681 gataacaacc agcaatgtta aagctgttaa ggtttagatc gaaagcgagt cccgataccc
     4741 tggctggcgg acgctcatac ttaatcagat agggtcgacg ggttttactg caacgcccgg
     4801 ttaataccac acagttttca tcgtatgcac gggctgtgtc atctttggca agtttgacga
     4861 tatagtcagt ttgtttaagg ggcggctgag aatcctcgcc aacactacga ctatgagccg
     4921 tgactgaagc tgtgatccac tggataaacc gcgaaaaatc gccttcctga ttttccgtaa
     4981 aggataagac gttttcagtc agttgcctga gtacactgag atcgaccgat gatccgatcc
     5041 ccacagcaat catgttcagt ttttttgcgt aaaattcgtt ccagcgctta acttcagctg
     5101 aaatgtcatc tgtcggtcga ccgtcagtaa gtaagtagac aactggcttc cagtcacctt
     5161 tttcttcctg cgtcgttttt cggacctgag aatctatctg tgttgtcagt tctcgtaggg
     5221 cagtcgcgag gttagttcct ccaccgacag gaaggcgggg gggataaaaa gacacaatgt
     5281 catgtaacgg gacaatcgtt ctggcaacac cagcaaaggc aataactgac acccagacag
     5341 tctccagagc atgagggtcc ttacgcagat cgcccacaat cttctgcaag ccatcattca
     5401 tccttttcag gttttcacca atcatggact cagaacagtc cagaacaaaa aacacgggaa
     5461 gtcgtctcac aggaaactcc ctcatagcaa aatgtgacgt ccatggcaga gatggacttt
     5521 atcggtatac ggcagggtta ttccctgcca taccggcgta agttacagaa caagctggat
     5581 ttcgggcggt ggcggtggaa gcgtgtccga atcagtagtg actccggcac tggagcttcc
     5641 tgaagccacg ctggcactga cccatttgaa aaagcctgaa aatgcagtcg aatccagtgt
     5701 ttcaagagcc acaactttgt cagtcagtag tttcagatgt tcgtgtcccg ctttaggacc
     5761 aacagcacag gctataatgg aaccaaacga acgccgctga acctctttca cagcttcccc
     5821 ataagcccag gcatctgacg gagtaccatc tgtcatcaga aacaccagcg gtctccagtc
     5881 gcctttctgt tcaccatcag agcgtcgaat atcccggtcc acacactgga tcaaacattc
     5941 aagcgccgcg ccggtaaacg taccgcccgc gcttggtact tctatatcag aaaactgaaa
     6001 gtccgccagg gcagtgagag gcacatactc ccgggcttca ttatcgtatg taattattga
     6061 gatatggacg ctttcgagcg cataagggtc ctgtctcagg gcactcagca tagcctgcac
     6121 acctacattc actgaatgta tcgattctcc tctcattgag ccggaagtat cgatgagcag
     6181 atatacgggt agtcttcgca tttagatata gttcctcaga tggcttacga acgaatcgga
     6241 ggtggaaggc tcgcctatcg cattcagttt ccagccactg gattcccgga caagctcagc
     6301 aaagaccatc gatcgctgac cattaaaggc tacaccgccg gagaggtcaa agcgtgccat
     6361 ttccacattt tttccgtcta cagcccggat aaatgcattt tgcacttttc caaagtgctg
     6421 ctgtaattct tcgccgttgt agatctgaac aataaagaca attttttcgt acttcggatc
     6481 gatgctgtta agttttacga taatttgctc atcgtctccg tccccggcac ctgtccggtt
     6541 atcgccggtc aaccagatct gtcctgattt atggcgaaga ctgttgaaga agacgatatc
     6601 gccgttaacc agtgtcggct ttccattttc aacattgcca aggtcagtca ctttaccggc
     6661 tgcgttgcag agaaaggcaa taacatcgag atcatactcc tcctcttttt tgccaaaaat
     6721 tccgccaagg aaaccttttt tctcttcatt gatgtcccag ccaagaccta tcgtgactga
     6781 tgaaagatcg aactcatttt tacgcaggct gacgccttgc cctttccgta agctgacagt
     6841 cataggatcc tctctttagt tggttactta taaaactacg tttacttctg gcggcggtgg
     6901 aggcaaatca tcaagaccga taacttcttt tttaccagca tcgactttct ggctacctac
     6961 ggaaacactg gcagacaccc atttgaaaaa agctttgatg gaagaactgt ctgccgtgtc
     7021 cagttggaga acaatctctg taatctcctt aagtacccct gtgtcggcat cgtgaccggc
     7081 agcacaggca acaacgacac cttttttggc tgctttgaat tcattcagtc ctttacgcca
     7141 gtcatcagta gggccaccat ccgtcatcag gaaaatgagt ggacgccagt cgccttttgt
     7201 ctccgctgtc gttttctgaa cttctgcatc aattcggttt gcagtcaggg ataaggcttc
     7261 gcccaaagcg gtggttccgc ttgcagaaaa ggagggcaaa ttgaaattga ggagatccgt
     7321 aagaggaacg gtctgacggg cagttgagtc aaaggtaatg acagagacat atgcggtttc
     7381 taaagcatat ggatcttgtt ttaacgtggt cagaagggtc tgaacaccat tttttacagc
     7441 ctctataggt tctccgtgca tcgaacctga agtatccaga agcagataga cgggtagtct
     7501 tctcatcttt gacattcctt aacgatttgg cgtacaagca cattgcttgt tcatcattat
     7561 ataaaaaata tttccttaca tttatgacat attacaaaaa acaatagtat ttagttcgat
     7621 taagagtcac aaaactagtg ttttatgtaa gttcagcttt gcaccttgtg ttctgaactt
     7681 ggtgcgcttg aactttttta catatttagg ggtaaccaaa taccactatt tttagtggtt
     7741 ggttagcacg ctaacactga agataagtgt actcgttaaa tactcatcac tcgattgtgt
     7801 atacaaatcc atgcccccgt agccagtcat ataactagac tacattctga ttcttttttc
     7861 tcaaaatcta attcatagat acttcacctc atcatttgta ccgctctcat tgcatgaact
     7921 tcggatactt ttccagtaga taccaggtgt gaaacttttc attgttcaag tgaaaaagtt
     7981 caacttaatc ttttcatgca aataccgtca tacagaattg ttatttaaat tatttgtaac
     8041 cataaattcg taatacaatg cagagaacta cttattacta cccagcagaa tatatcagaa
     8101 gttatgaaag aaatctgttt tagatgcctc attagtataa ttgacaagtg cgggtgtaag
     8161 tccgagtttt tacattagct ctttaatgcc attttgtttt gagcataaaa ttcgattgac
     8221 cgctaaatct gcaatctgta tgatgtgatt agggacagca accaaaaaaa attgctgata
     8281 ccctaagagc catgagaagg aaaaatataa tatggcaaat cttaaaacaa aaaatagtat
     8341 cagaaggcaa ctgcgttggc taagaaatct tcgtgattat tatttagatg atagggaatt
     8401 tgttagaatt ttgagcaacg aaatccgtgt attagagaag ttactatatg atattgtaat
     8461 ggaagaatac aacccattga caacacgctt tgataaatgg gattagcttt tctccaacat
     8521 cactagttat agtagtggtg ttggttttta tttaataaag aattctccat tattaaataa
     8581 ctgccgctcg tatgactaaa tgttaatcca tctacttcat ctctttctta cgaggatttt
     8641 tcctaacacc aaatagttat gaacttattt cctgtctttt gaaataccga atacacatta
     8701 aaatcccgga gataacgttt aaaaccataa attagtggag ttagtgctaa cactccacta
     8761 atgttatatt attttttcaa gacctttata atttcatcta tcttcatttc gaccagtttt
     8821 tcaactgctt cgcttcttga tgttccgctc agctctgcca gtttatctat tttccggaga
     8881 atacggcgtc taatcgacag ggaaacggaa gtttttatat ccttatccag tatcacccgc
     8941 gccccctggt catgtgtttt taaatcagta ttaaggtcca gcgcctcact cattctcttg
     9001 agtgttttct tatccagtaa tccggagttt accatctgat aggagtggcc agccttactg
     9061 tatttaattt cagcagcgta ggtttccctc agctctttaa gcgcccggct tagagtcggc
     9121 tcagaacagg ccagcgtcgc ggtgatggat tgcgccgata caggcttccc tgagcccagt
     9181 atgttagcca gggtgtatac tctttcctgt ctggtgttta attgcatatt cagtcggtac
     9241 ctgccatatg ggtcagtggt tctataggct gaaaaatgta agtcgcactc attttcctga
     9301 tcagacctcg atcacctaat gcttcaatgg ggtcaaggcc acgcagattc ccgaagttga
     9361 ttagctcgat ttcagagcgg catacccgcc aggaaataat gtactcgtct tcaagatatc
     9421 cagtgcgttt acttacccgc tgagtgaagc ctgaaatgac gacctgttca aacggaagcg
     9481 tctgaaaaac gattgctgct aatctcagca ggcatccgtg aacgtgttta gcgtaacttt
     9541 cccgggccgc tttctgcgtc atggcttttt cattgatatc agttccccgg gcattcacgc
     9601 tatacacttt atcaggcata tcctcaatct caggtaaatc aacatcaagc cagactgttg
     9661 attcctcagg tcgtacctca aacgacacca gtgtttctcg cggccattct gtttgcgaga
     9721 aaaattcctc aagacaatgt tcaaagaagc gggcgtcaga tcggaattgc tctttcgcta
     9781 aagctacaga ctccgtgcga tttttttcat gctcctggta ctgatgctgc cattctgcgt
     9841 actgctcagc ccaggctata cgctgtttct cataacgctg cttcagcaac tcattttcac
     9901 gttcaatatc agcgactgtc acctgccacc gacgaagatt ttcgttctgt cgctcctgac
     9961 gggctgcatc agattcaaac catcccccaa ggaagccgcg accggcatgc tcatcaggtt
    10021 ctggtggaag tggtatatat tcaggtttgt ttggtcgtac cggcgcaggt agactgaaac
    10081 tgccctgacg gagcgccagg tactgtgtct cgagcatggc ccaggtattc cctccctttg
    10141 gggaagggga gagttcatgg atattggtta tcactgttac ggtggacatc agtttttcca
    10201 ccaccagctc cagttcagac ttcaaaccgg gatctgcctg atgcctggta ttcactcttt
    10261 cccgtgctgt tctgtcaatc cgggtgcggt aactcagtcc cgttccggga agtcccaggt
    10321 tggcaaatgt tccattcttt cctactgaca gcgaggctcc acggggacca atactcaggc
    10381 tgggggcccc gttactcagg ttaaccctga cccccgggat aatgctgatc gatttacgaa
    10441 atctgaatcc catatatgct tcctcagtgc tgcttagaaa ctggaataag cctgattcct
    10501 tccggatttt gcccggtagt cagaccgtat acctgtcccc gtcccaggat cccctgagcc
    10561 cacctgcgat gggtagccgg ttcacacatt gctccctgag atttaaagac tgcctgggtg
    10621 gaattgagaa ttcgtaacgc atctgaatgc tcaccaggag acacctttag cgcctcctct
    10681 atcacactaa tctgatcagg atgaatggcc gttttaccca ccagcccatg ggcgatatcc
    10741 atcgagagct cccgtcccag aactccgtga tcgtcgatgt gttcgcatac tggtgcggta
    10801 agtgcaaaat cacgaggtgc gaatactgac acaagcattt ttatgacata acccatagga
    10861 gtgtcataaa gggtcagatg ccgtggacgt cgcagagata ccacgttcat gagatcgttt
    10921 ccgccgatcc gcaaagcgat tatacggtca cggcagtcat gactcgaaag ttcatttgcc
    10981 agcgcctgca ttttaataac atcatagact tcttcagttt caagtgttgg catcatcatc
    11041 aatgatgttc cggccatgat gtcccaccaa accgggagtg tggaacgggt gaatttgggc
    11101 agtacaaaac catcaactgc actcaggtcc aaattagccg ttagccatct ccccatatca
    11161 gggtggcgtg gacggataaa taccagtggg tacttctcat catcgttgac ggctcttgcg
    11221 tgtctcaatc gctccaaaaa cagtgaaaga ttctgaatcg caagagggat gtcatgctcg
    11281 ctgaccgcat cttccaggca gacaaccaga gagcgtagcc ccggtatttt cccttccaga
    11341 acgacctcag cgatatcctt gcgtgtggca ggcatataca gcgtggcacc gagttcccat
    11401 gggttgggtt gttttttcat cagcgtacct ttttgataat ggtaaccgca cgatactgac
    11461 cgagcgtatc cccggcttcc gtcacctgga tacctttctc ttttgccagc ccaaccagaa
    11521 gagaaacatc cgggtcatcc atctggcgaa caaaaacatg atcaggtaca cgacgcagaa
    11581 ccgcccgcgt cgcttcagcg atacccggct taatgcggtt aatgctgtcc acctcataac
    11641 gcgcagcaag gctctggata acttttctgc tcagctgcca gcgtgcggca tttttccctg
    11701 cgtcccagcc agaagcggga aggggtttac cgtcaagctg ttcacggaaa tgtgctaccg
    11761 tatccaccag catctggcta cattcaaaag actgaaggtg atcgcaaacc acacagccat
    11821 gcaggccttc ttcggcccag acagagcgcg aaatcagacc cgagacaggc gcgcccatga
    11881 taccgaacgg gataagccag tcatcgtcac tggcagcaag ccaggcacat ccgcaggggt
    11941 cagccagcac aaccagtcga ggctgctccg gataaccggg acggtccttc agtgtgcggg
    12001 ataattcgcc ggtaatagct ccttttcccg tccagccgtc gacaaacact atgccaccag
    12061 taccatggcg tgattcgata aaatcaagcg ccgcagcatc aatacccctg tcgcggataa
    12121 tgctgatccc gtagtggaaa gactgttttc ccatcgcacg cagagcctga tgcagcatga
    12181 cccccagtgg aacaccagct ctgaccaggc tgaccaggac aatgggttca tcgccagcac
    12241 gacgggccag ttcacgagca agataagata cctcccgtgc cagacgtgca gctcccgagt
    12301 caagcgcacg ggtgaaaaga tccaggtgcc agcgggtcgg ctctggttcc tggcttaaca
    12361 tgtcggaata atgcattttt cctgactgaa tcagttcttc cttcaggtca accggtgtca
    12421 tctccatgac aatgggtttc agcaaaaagt gaacatcgtc cggtttatag gaaccagaaa
    12481 acggggcaaa atgcgacatc ttattgctct ccaaaaatac gctgtgatat ggcctcggca
    12541 aactgactct ggcgtggaag accaaaccag ctgcagagtt tcatgaaccg atggtcgctc
    12601 aggctgtcac ccagccccat caccgggaaa accccgcgct cctgacgcag tttctccagc
    12661 aaccatgaaa cggccagccc tttttctacc gggcagggta accaggcaat gttgttgctg
    12721 ttacggtgga tgtaaaaccc ttcggtcgaa aaaacgcgct caatctcatc cccgacagcg
    12781 ttcagttcat caagcttcgt gctgtcgcgg tgcttcatga ccagatacac cggcgtaccc
    12841 tcatattcgt agttgaggcg tgcccaggca ttaatgcctc gcgcatccat gagctcggtg
    12901 atgatatgtt gcattgatgt taagcggtcg gcgtaggagg acaggcactc cagcatatgg
    12961 gctttccagt cgctgtctgg cgtaccatct ggacgaagga tcaccgcgcc atgagtggtc
    13021 acggcccagg aacggaaggg gatctgtacc cgactgattt cttccgtacc tctggctgtc
    13081 accggaatga ggtccgcatg ctccagcaac cagtccacca gcatggcctg ctcttcggtc
    13141 ataaagctgc gaggcgtgag gctgcgatcc agtgcgccgg tccggaacgg ttcaagggca
    13201 agttcatcaa ccatttttcg gcgggtctgg aacagggtgt catctaggtc gctgaatact
    13261 acgggcttat tcataggtaa tcacctctaa ggtgggagcg acagcggaaa gcgcctcaag
    13321 cagccggggg tcgacgctgg caactggcgt ttcgcaacaa atcaggatcc ggtcaaactg
    13381 ctgatgagca acgttataga cataattcgg aatacccagt ccgtagttat cgctgaaaga
    13441 aatggcggac tggatagcat aaccagtgga aatcggtgag cgggtcgtcg aactgaacac
    13501 gacctgagca cctgccgtct caagtcgttc agccagcagg aacggctccc agacaaattc
    13561 accggtgccc agcaccagaa tgttttcacc gggcgaaacc tgaacctgaa gacccaggtc
    13621 actcgccggt atctgcatcc cgagtcgccc ccaggattgt ttgccggtga tcgggatgtt
    13681 ccctctggag gtcacattta cctggggcat gacaggcagc ggggcatctg gttctggagt
    13741 ccagcgccag ctgcctgaga tgagcgatac tgtatgcagg ggcagggggc agttctgata
    13801 gagggcttct ccgctccagt cagtcagcgt gacagcaata acccgtcgta ccttccggag
    13861 tgcaccggat gcacgtaatg cggcgagaag attgaggaac gtttttcccg tcgtggcttc
    13921 atcatccacg agcactaatg tttgagcctc cgctacggtt tgccgcatcc cggcacaaac
    13981 tggatagtag atcaggtggt ctgtggcatg actgtgctct tctttaaact cgcacagcag
    14041 ttcagcatca accggatgcc gtgttgatgt cagatagaca cagtcaggtt gatgcttacg
    14101 cacctcatca aaaactcccg cccctaggcc gacagccgtc tcagccatcc cgataaatac
    14161 caccggccca tccagagtcc cgggaaactt ctccgccagc tgtgtataaa catttcgcat
    14221 gacctgagga gagacaggaa tatgtcgacc cagcacttta ctgacaaaca gaaatgcgcg
    14281 ttttggatta cgccgttcgg cgatatcaaa cagatcagta agaggataat cggtgacctc
    14341 gctgcttacc tcaattcgcc cacaggataa tgtcttttga taaacgaaat tcccatcaat
    14401 tgctgacata tctgtctcca ttaatcgatt ctgttggtca atgatgccgg atagggaata
    14461 acatcggtga cagagcggac gactgcaggt gaaaaatcag ttttggctga ctgccggacc
    14521 tgttcatcat ccatcagtcc aagtgtatgg aaggcataga gacccggcag attcacgcca
    14581 ctgtgccgcg tataaccgat acctcctgaa ggccgcatgt taatttccag caatagcggt
    14641 tttccttcgg cattatggcg tgtctgtacg ttgaccatgc catctgcgtt cattaggcgg
    14701 gcgcagtcga tggcaagttc aatcgcttcc ccttcattct ccagatactg aagcacacca
    14761 accttacgcc gcccaatagc ggccaggacc gtcccttgac ttaccaccat atccacggag
    14821 tattctggcc cgggtaaata gggcatcagt acttgtggag taaagcagtc catccgggca
    14881 tgcgcagcca gatactgtga tgggtggata acacggttat cagggttgtt gagaaaagct
    14941 acaggagaga cattgtcatc aaaacgccag aatcccatgc cataaatgcc tgtgacaggt
    15001 ttaatacaga ggcaatcaga agggaaaggg ggatcactca gtagcgcgcg aagctcgtca
    15061 acatttttta cccgaaccga cgggacaacc ggaagtccat ttttttccat aaactcggaa
    15121 aaagtaactt tttcgtcggc taattcgagc cagtgggtac ccgttgaccc ggtagtcagg
    15181 tggacaccag aacgttgaat gttttcccgg tggctttcaa accatttaca gttgcgacca
    15241 gtatgtatcg cattaatacc atgagcactc gtaattgaat ggataaatga aaggcgttct
    15301 tcttcacttt ttggttcgag cattgaaaaa tcggagactg acaagatctc atttctttca
    15361 ttgcgatgag acgaaaaaaa aacaatattc tgattttcct tttccgcaaa tgattttacc
    15421 ccaagaacaa tgtctcgctg ggaagatagt ccttccataa accaaatttt cttagtcatg
    15481 ttttataatt atcgcctgat gagaggatgg tcgttttatg atacttgagt ctaagaacac
    15541 ccacgcaccg tgtcaatcat aatatgataa acactgtgtg ttttcgaatt tcttaaaaat
    15601 aaaataataa tgatatatat caacagcata catgagaata aaatcaggag tgacaatgta
    15661 atgaaacgta aattatgaaa acctaccagt tttaatcttg cgagtaaact ttgtcatgat
    15721 atgattttta tgttgcttca ctgaatatga attttaaaaa acctcagtca atacctttaa
    15781 ggaatgaata tggtttcttt aagtaaaaac cagacggtat cactcagcaa acaatccacc
    15841 gatctcagcc agcttcagtt cggattaggt tgggatcctg ttaagaaaaa gggattattt
    15901 ggcagctttt ttggtggcaa tgattccata gatctggatg caggttgcgt tcttctggat
    15961 gtggcaggta ataaaattga caccatctgg ttccgtaaac tgaaatccag ctgtggttct
    16021 gtcgtccact ccggcgataa cctgacgggt gagggggatg gcgatgatga aatcattcac
    16081 gtcaacctct taaaattgcc ggcaaatgtt gaatacctcg cttttactgt gaacagcttc
    16141 cgtggacagt ccttcaacga cgtggataac gccttctgcc gcgtcgtgga tcagaccaat
    16201 aaggaacttg cccgctatca gctgacagaa caaggcccac ataccggcat cattatcgct
    16261 tccctgcgcc gcaacaacgg ccactgggac tttaccgccc acggtaaagc aagcggtggc
    16321 cgcacaatcg acgatatgca gcgcgatatc atcagtacgg tggtaaaagc atgaatctga
    16381 caccgggggg caatgccccc gtacctgctc aggaactgcg cgttagagtc atttctggtg
    16441 gtcctgttga tgcctcggca tttcgattat tcgctgacgg gaaagttcgg ggtgacagcg
    16501 atatggtctt ttacgggcaa ccccgtaatg aagatggttc cattagcttc agtactgaag
    16561 ggaccaatag cgttttcacc gttgatctgt cccgcctcaa gcccgacgtg cagaaagtcg
    16621 cgttcaccgt tacctgtgac ggcagtcata cagtttcctc cctgaatcat ctgtccatac
    16681 aaattgaaag tggcaatacc tcgctgatca gtggtcaggt agaactgagc ggccgtcagg
    16741 aagccgctct gatcctcggt gaagtctatc gcaggaatgg tgactggaaa tttcggttta
    16801 ttgcccaggg cttcaatggc ggcctgaaac ctctcgctga gtactatggc gtcaacgtcg
    16861 cggatgagcc agctccagtc actccgccac cagcgcctcc ggctccgcct aaagttagcc
    16921 tgagtaaagt ttctctaacc aaagagaagc ctgccatcag ccttgccaaa aaagacaatt
    16981 ttggtgaaat ccgcatcaat ctgaactggc atcgaggctc gggttcaggc gggttactgg
    17041 gcggtatgtt tggttcgaac aaagcgatag acctggatct cggtgcattc gttgagctcg
    17101 cagatggcta taagtcagtt gttcaggcac tcggtaatgc ttttggtaac tatcactctg
    17161 agccatatgt tcaactgcag ggtgatgatc gtaccggcga ggctctggat ggggaatggc
    17221 tacacatcaa tggccgcgaa tggaaagaaa tccgccaggt actgatttat gcctttatct
    17281 atcagggcgt gcccagctgg gacaaaaccg atggcgtggt gacattacat gttcctgagc
    17341 aaccaccgat tgaatcgcgt ctgactgaag gtgataacaa acgcacgctg tgtgcgattg
    17401 cccgtctggt caacgagaac ggttccattc gtgtggaacg tattaaccag tacttcagag
    17461 gccaggagga aatggaccgc gcttttggct gggggtttcg ctggtccaga ggttccaaat
    17521 aaacgcaatg atgagggtat gagaatgagt tttttcgaca aagtcaaagg ggctttgacc
    17581 tcgggtcgtg aggaattgac ccgccaggta ggccgttaca aaaacaagaa atttatgcag
    17641 ggtaccgtcg cggtatgcgc ccgtatcgct gttgccagtg acggtgtcag ctccgaagaa
    17701 aaacagaaga tgattggatt tctgcgatcc tcagaagagc tgaaagtctt cgatacagca
    17761 gaagttatcg aattttttaa caagctggtg accagtttcg actttgatct ggaaatcgga
    17821 aaaggcgaaa cgatgaaata tatcctcgcc ctgaaagacc agccagaggc agctcaactg
    17881 gctcttcgtg tcggtattgc tgtcgctaaa agtgatggca acttcgatga tgatgagaag
    17941 agtgctgtcc gtgaaattgc ccgttcgctg ggtttcgacc cggctgaatt tggcctctga
    18001 taaacaacgg ataaaggtac gatatggttt ccactcacat cggttttccg gctgaaacag
    18061 tgacggtctt tgttgcgctg tcggtcggcg ccatctttat tgacctgttc atgcaccgac
    18121 atgacaagcc aatttccctc aaaagcgcag ccttatggtc cgttttctgg gttgttgtcg
    18181 cgatggcatt tgccggtttc ctttttgtgc atcacggagc cgaggttgcc agcctgtttg
    18241 tcacgggtta tgcccttgag aaggtgctgt cagttgataa cctgtttgtc atgatggcga
    18301 ttttctcatg gttctcagtt ccggaccgtt accgtcaccg ggttctgtac tgggggatca
    18361 ttggcgctat cgtattccgg gggattttcg ttgcgatcgg taccggtctg cttagccttg
    18421 gcccttatgt cgagctgatc tttgccgttg ttgttgcctg gactgcggtc atgatgctta
    18481 aaggcggtgg ggatgatgac gaaattgagg attattctca gcaccttgct tatcgcctgg
    18541 ttaaacgctt cttcccgatt tggccgaaac tgtccggaca tgcattcctg ctgacacaaa
    18601 aagaagtgga tgccgaactg gctaaaccag aaaacaaaga cgtatctgta gggcgcctga
    18661 aaaaggcagc gctttacgcc accccattga tgttatgtgt ggctgtagtg gaactctctg
    18721 acgtgatgtt tgccttcgac tctgtcccgg caattattgc agtcagtcgt gaaccactga
    18781 ttgtgtatag cgccatgatg ttcgcaatac tgggcctgcg tactctgtat ttcgtactgg
    18841 aagccctgaa acagtatctg tcccagctgg agaaagctgt tatcgtcctg ctgttcttca
    18901 ttgcatttaa gctgggtctg aacgctaccg atcacatctg gcaccatggt tacagcctgt
    18961 ctgcgaccac cagcctgtat gtcgtactgg gtgtactggc tcttggtatt ctggccagtg
    19021 tcatctttcc ggaaaagccg gaagatgaaa ataaagaaag ctgatctcag ctgattaatg
    19081 attaacgaaa gaggataaat agatgagcgt ttcactctct aaaggcggca acgtttccct
    19141 gagcaaaacc gcgccaagca tgaaaaatgt cctggttggc cttggctggg atgcacgctc
    19201 tacggacggt caggactttg acctcgatgc ttccgcattt ctgctggcag ccaatggcaa
    19261 ggttcgtggc gatgcagatt tcattttcta taacaacctg aaatctgctg atggttccgt
    19321 tactcatacc ggtgataacc gtaccggtga aggtgatggc gacgatgaat ccctcaaaat
    19381 caaactggac gcggtacctg gggatgtcga caaaattatt ttcgttgtca ctatccatga
    19441 tgctcaggct cgtcgccaga gcttcggtca ggtttccggc gcatttatcc gtctggtgaa
    19501 tgatgacaat cagactgaag ttgctcgcta cgatctgaca gaagacgctt ctactgaaac
    19561 tgccatgctg tttggggagc tgtatcgtca caacggagaa tggaaattcc gtgcggttgg
    19621 ccagggttat gctggtggac tggcttccgt gtgcgctcag tacggcatca acgcgtcctg
    19681 ataaaaagta actttcttac ccaaatcatt cgggggtgat gcccccgata tccacgaccc
    19741 gatatctacg attagcagga gcaaaaaatg gctgtttctc tcgttaaagg cggtaatgtt
    19801 tctctgacta aagaagcccc gagcatgaat gttgctctgg ttggcctggg ctgggatgca
    19861 cgcgttactg atggtcaggc atttgatctg gatgcatcag tattccttgt tggcgaaaac
    19921 ggtaaagtgc tgtccgattc gcatttcgta ttttataaca acaccaccag cccggatggc
    19981 gcagtacagc atcagggtga caaccgtacc ggtgaaggcg acggtgacga tgagcaggtc
    20041 aaaattgatc tgacaaaagt ggccgcagac gttaagaagc tcgtattcgc agtcaccatt
    20101 catgaagcag acagccgtaa gcaaaatttc ggcatggtca gtaacagctt tatgcgtgtg
    20161 gtgaacaacg ataatggcgc cgagattgca cgctttgacc tgtcagaaga tgcctcaact
    20221 gaaaccgcaa tgattttcgg tgaactgtat cgccatggcg cggaatggaa gtttaaggct
    20281 gtaggacagg gctttgctgg tggcctggct gcgctggcaa cccagcacgg catcaacatc
    20341 tgataaacca gtaaaaccaa accccggcct gtccggggtt ttttctaccc gctgagtgat
    20401 ccctttaccc ggcaacaagg agcctaaatg caattacaat caggacagaa cattccgctt
    20461 cacacagata cactgactct ccggcttgat tatcccccca gtcctggatt tcacagtacc
    20521 cctgagactt gcctgtttat gctgaatgct gagggaaaag tgacaggcga tgccgatttt
    20581 atcttttaca acaatctcag tgctgcgggt ggcagtgtga aactcaaccc cggtcaccag
    20641 cacgcagtcg ttcattttgc gcttgaccag atcccggata cgattcagaa gattgcaatc
    20701 actatggtga tagatggcag tgatacgata agcggcctcg cgcaactgaa actttctgct
    20761 gaaagtcagg ccaccttttt tgtcgatctc aatggccgca cagaaaaggc agtaattatg
    20821 ggggaagttt accgatacca gggcaactgg aagctcaggg cgcttgggca gggatttaat
    20881 ggcgggcttg agccactggc agttagctat ggtgtggacg tggaacctgc agcagaagtt
    20941 gtaccgcaac cagccaggat cagccttgaa aagaaactcg aaggtaaagc gcctgcgctt
    21001 atcagcctgg ctaagaaagc cagtatttcg ctggcaaaac acaaactgga taccgttgaa
    21061 gcacgagtcg cttttgttct cgatgcctca ggctctatga ccggccagtt taaaaaaggg
    21121 catgtacagg ccgttcttga ccgtatagct gtcctgtctg tccagttcga cgatgacggc
    21181 acgatggatg tatggggctt tgctgagcgt cacaagaaat atcctgacgt cacactggac
    21241 aatcttgacg gatacattgc tgccattcag aacacaggta aacgatctgc ctgggaaatc
    21301 cttcccggcc ttggcgggac gaacaatgag ccgccagtca tgaatgaggt tatcgattat
    21361 ttcaaggaca gcaccttacc tgtttttgtt gtgtgcatta cagatggcgg gatcagcaaa
    21421 acacgagaaa ttaaagaggc catccgccgc tcagccaact atcctgtctt ctggaagttc
    21481 gttggactgg gcggatcaaa ttatgggatt ctggagaggc tggacacctt tagcgaccgt
    21541 cgggtagaca acagcaactt ttttgcgatc gataattttg cccacataaa ggacgaagag
    21601 ttgtatgaaa aacttctcga agagttcaaa gactggcttg gactggcaaa aaaggaaggc
    21661 attatctgaa tcatcgcccc ggtaaggggc gataggcctg ctgccatgac gatacccttt
    21721 cataatacgc agagctaaac agggctatac gcagatgtga tcgctgaaac ccaacttcac
    21781 ccagtaattt agacccagga acacatcggc agggatgaaa aaaacccgct cggaaaagcg
    21841 ggctgaaagt aggtattcca atggtaacag gttgaagcat acctaatagt ccgagctacc
    21901 gatttaccag gatgcactct ttttttgacc agttacattt gttaaatgta gaatatatgt
    21961 tacctttatg taaagaagaa attgcagaac gataaagggc aatttttatc tgatgattag
    22021 cttcgacgga agtttacgac ggaaaccttg atggaagtga cgccagtgag gctcagtcgc
    22081 tgcggaaagg agttcataat caccccgcgt atcaccccat gcccgaagat gatagtcagt
    22141 cagaggtcca tattcttttt caagccggat aattttctgt gagcacctgc aattatgacc
    22201 actgattctg ccggtcagaa tgccgtcttt tgtctctaat tgtgtaccaa tcagtttgat
    22261 cctaagtttt tcggcaaagg gtctgagtac aatttccggc gacgcagaac ataaggtgac
    22321 gatcgcgccg gaagtgactt cattcgccac agcaatcaaa cctgccgggc gcatgaggcg
    22381 atgccagtag agctgacaga attcctctgc tttagaagac acccactttt cctcaacacc
    22441 agtaagaaaa gttgtaatga gcgtttcttt tagctcatca cgcgtcatct gcttacccat
    22501 acagcgtaac gtgggtagag ccatgctgac gatacgtcgc gaaaacgccc gattgccaaa
    22561 ggcaaaccgc agaaaaggga taaagctatc atgccgcgtt aacgtaccgt caaagtcaaa
    22621 tacagagagc atgcggggct gaatgacgtt catcgtgatc ttattccgtt ggatagtagg
    22681 ttacttctgc cagccgctgc actataccac caggacgcga ataacagtaa ccgcacgtaa
    22741 ggtgctgaca tcctgctcgc gtcaaaatga agggcaaggg tccaggcacc tcctgagaaa
    22801 aaagtaactt tttcgtgatt gtttgctcag taaaatctga tgcggtaacc ccatactctg
    22861 gatcgacgga gggcagtata ttactgttcc ttcacaagta tagagttcag acctctgtag
    22921 tcatacctat acagatggta cttcaagttg ctggagcctg tgcccatggc gctgcggctt
    22981 tgttgaataa atcgaacttt tgctgagtt
//
LOCUS       DAOFQD010000072        22049 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.072, whole genome shotgun sequence.
ACCESSION   DAOFQD010000072 DAOFQD010000000
VERSION     DAOFQD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.072"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..194
                     /locus_tag="QBF46_002887"
     CDS             <1..194
                     /locus_tag="QBF46_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6675048.1"
                     /translation="TAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEM
                     VQSGRPLKQEPTEMIQATA"
     gene            complement(234..1448)
                     /gene="ydcO"
                     /locus_tag="QBF46_002888"
     CDS             complement(234..1448)
                     /gene="ydcO"
                     /locus_tag="QBF46_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDQ6675049.1"
                     /translation="MLRYLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLALGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPSYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSTPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1501..2037
                     /gene="sutR"
                     /locus_tag="QBF46_002889"
     CDS             1501..2037
                     /gene="sutR"
                     /locus_tag="QBF46_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDQ6675050.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATRVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2110..4071
                     /gene="rlhA"
                     /locus_tag="QBF46_002890"
     CDS             2110..4071
                     /gene="rlhA"
                     /locus_tag="QBF46_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDQ6675051.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4163..4393)
                     /locus_tag="QBF46_002891"
     CDS             complement(4163..4393)
                     /locus_tag="QBF46_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDQ6675052.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4615..4791
                     /gene="hicA"
                     /locus_tag="QBF46_002892"
     CDS             4615..4791
                     /gene="hicA"
                     /locus_tag="QBF46_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDQ6675053.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4837..5253
                     /gene="hicB"
                     /locus_tag="QBF46_002893"
     CDS             4837..5253
                     /gene="hicB"
                     /locus_tag="QBF46_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDQ6675054.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5332..6738
                     /locus_tag="QBF46_002894"
     CDS             5332..6738
                     /locus_tag="QBF46_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDQ6675055.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQTLELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSQAALAHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6983..8128
                     /locus_tag="QBF46_002895"
     CDS             6983..8128
                     /locus_tag="QBF46_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ6675056.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8146..9159
                     /locus_tag="QBF46_002896"
     CDS             8146..9159
                     /locus_tag="QBF46_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6675057.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDSVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9160..10101
                     /locus_tag="QBF46_002897"
     CDS             9160..10101
                     /locus_tag="QBF46_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6675058.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            10091..10885
                     /locus_tag="QBF46_002898"
     CDS             10091..10885
                     /locus_tag="QBF46_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6675059.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10907..12331
                     /gene="patD"
                     /locus_tag="QBF46_002899"
     CDS             10907..12331
                     /gene="patD"
                     /locus_tag="QBF46_002899"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDQ6675060.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12428..12523)
                     /gene="yncL"
                     /locus_tag="QBF46_002900"
     CDS             complement(12428..12523)
                     /gene="yncL"
                     /locus_tag="QBF46_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDQ6675061.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12718..12891
                     /gene="ortT"
                     /locus_tag="QBF46_002901"
     CDS             12718..12891
                     /gene="ortT"
                     /locus_tag="QBF46_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDQ6675062.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            12977..13210
                     /locus_tag="QBF46_002902"
     CDS             12977..13210
                     /locus_tag="QBF46_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDQ6675063.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13211..13660)
                     /locus_tag="QBF46_002903"
     CDS             complement(13211..13660)
                     /locus_tag="QBF46_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ6675064.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSNGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13657..14175)
                     /gene="mddA"
                     /locus_tag="QBF46_002904"
     CDS             complement(13657..14175)
                     /gene="mddA"
                     /locus_tag="QBF46_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDQ6675065.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTLAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14355..15392
                     /gene="curA"
                     /locus_tag="QBF46_002905"
     CDS             14355..15392
                     /gene="curA"
                     /locus_tag="QBF46_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDQ6675066.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15590..16255
                     /gene="mcbR"
                     /locus_tag="QBF46_002906"
     CDS             15590..16255
                     /gene="mcbR"
                     /locus_tag="QBF46_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDQ6675067.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16291..18393)
                     /locus_tag="QBF46_002907"
     CDS             complement(16291..18393)
                     /locus_tag="QBF46_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDQ6675068.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEGMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTIGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYITPSNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLIGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            18635..19696
                     /locus_tag="QBF46_002908"
     CDS             18635..19696
                     /locus_tag="QBF46_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDQ6675069.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(19811..21310)
                     /gene="ansP"
                     /locus_tag="QBF46_002909"
     CDS             complement(19811..21310)
                     /gene="ansP"
                     /locus_tag="QBF46_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDQ6675070.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            21574..>22049
                     /locus_tag="QBF46_002910"
     CDS             21574..>22049
                     /locus_tag="QBF46_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDQ6675071.1"
                     /translation="MIKMYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLV"
BASE COUNT         5603 a         5438 c         5697 g         5311 t
ORIGIN      
        1 atacagcgaa agagaaccgc ctgtcacaaa gtaaattcag atgccaggta tgtggatata
       61 cagcgaacgc cgatgtaaat ggcgctcgca acattttagc ggcggggcac gccgttcttg
      121 cctgtggaga gatggtgcag tcaggccgcc cgttgaagca ggaacccacc gaaatgattc
      181 aggcgacagc ctgaacgtag cagggatcca cgtccttcag ggcgtggagg atgtcaatat
      241 cggtttctgt cagcgattaa attcaacacc acgtaacaaa cgcctccggc aattaatccc
      301 caaaacgcag aaccaatccc gaccagcgtc aatccacttg ccgttaccag aaatgccacc
      361 accgccgcgt cgcgctcacg ctcattatgc agcgcctgat acaaactgcc gccaatggta
      421 ctcaacagcg ccagacctgc cagcatctgg atccaactta cgggcagggc agccatcatc
      481 ccggtaatgg cactaccaaa cagacctgcg agcaaataga aaatgcctgc aacggcagct
      541 gccagccaac gttgatcttt atccggatgc gcttccgggc tttggcaaat agccgcggtg
      601 attgccgcaa taccgacgga ataaacgccg aaaggggaaa aaaccagtgc cagcaatcca
      661 gtaaatacaa ttaatggcga aacaggagtt gaatatccgg ctgctttcat tgctgcgata
      721 cccggtgcgt tttgcgatgc catcgtcacc agaaaaaggg ggagtgcaac gctcaggctg
      781 tgagcaaacg aaaaatcagg ggtaatataa gaggggagaa cgggtttaaa gacaacatca
      841 gttgtgacaa cgtcaccttg cgcgataacg atcacgatcc caataatcat cgcggcaatt
      901 accgcatagc gcggcgcaac ggccttggtt gccagccata ccagcaacat acttccacac
      961 aacgtaaatt gaccgtccag actggcaaac gcctgtaaac caaagcgtaa taaaatcccg
     1021 gcaagcattg ccgccgcaag cgagtgcgga ataatgcgca tcagacgagc aaagagtccc
     1081 gttatgccgc agaggactat taacgcgttg gtgacaataa aaacgccgat ggcttcgtta
     1141 agtgttagtc cctgcaatcc ggtgaccaac aaagccgcgc caggcgttga ccatgcggtg
     1201 agaacaggta cgcgatacca taatgtcaga gtcagcgtac tgacgcccaa tgccagcccc
     1261 agcgccgtca tccagccaga tatttgtgca gtggtggctc cggcgacaat cgctgcttgc
     1321 cagattattg ccgctgaact ggcgtagcca attaataccg ccagaaaccc cgccagtagc
     1381 gtgggtggag gaacagaaaa cagacgcata gtcacctcgt gcgttatagc gtacaggtac
     1441 cgtagcattt gtccgttata acgcacaagt gataaacttc cgttttgccg gaggagtcgc
     1501 atggaaaatc tcgctcgctt tttatccacc acacttaaac aactacgtca gcagcgcggc
     1561 tggagtcttt cgcgactggc agaagcgacg cgcgtttcta aagcaatgtt aggacagatt
     1621 gagcgtaatg agtccagccc gacggtcgcg acattatgga aaattgccac tgggttgaac
     1681 gtgccctttt cgacatttat ttctccgcca cagtccgcta cgccttcagt ttatgatcca
     1741 caacagcagg cgatggtgat cacttcgctg tttccttatg atccacaact ctgctttgaa
     1801 cacttctcga ttcagatggc acccggtgca ataagcgaat cgacgcccca tgagaagggg
     1861 gtaatcgaac atgtcgtcgt cattgatgga caacttgatc tgtgcgttga tggtgaatgg
     1921 cagagcctta attgtggtga aggcgttcga tttgctgcag acgtcacgca tgtctatcgt
     1981 aatggtgggg agcaaaccgt acattttcat tccctcatcc attacccgcg cagttaagcg
     2041 ggaaaactat ttcgcaacgt cgcgcttgtg gctaaaatag ccgccatttt tcagctactg
     2101 gataagaatg tgaccgtatc ttctcatcgc cttgaactgt taagcccggc acgcgatgcc
     2161 gccattgccc gcgaagctat tttgcacggt gccgatgctg tttatatcgg cggccctggt
     2221 tttggtgccc gtcataatgc cagtaatagc ctgaaagata ttgccgagct ggtgccgttt
     2281 gcccatcgtt atggtgcaaa aattttcgtc acgcttaaca ccattttgca tgatgatgag
     2341 ctggaacccg cgcaacggct gattactgac ctctaccaga ccggtgtcga tgcgctgatt
     2401 gttcaggata tggggattct ggaacttgat attccgccga ttgaactgca cgccagtacg
     2461 cagtgcgaca ttcgtacagt agaaaaagcg aagttcctct ctgatgttgg cttcacgcag
     2521 attgtgctgg cgcgagagct gaatcttgag cagatccgcg cgattcacca ggctacggac
     2581 gcgaccattg aattctttat tcatggcgca ttgtgcgtgg cctattcggg tcagtgctac
     2641 atttctcatg cgcaaacagg gcgtagcgcc aaccgtggcg attgctcgca ggcgtgccgt
     2701 ttgccataca cattgaaaga cgatcagggg cgggtggttt cctatgaaaa acatctgctg
     2761 tcgatgaaag ataacgatca gactgccaac ctcggcgcgc tgattgatgc tggcgtacgc
     2821 tccttcaaga ttgaagggcg ttacaaagat atgagctacg tgaagaatat caccgcccat
     2881 tatcgccaga tgctggatgc cattattgaa gaacgtggcg atctgacgcg tgcttcatca
     2941 ggtcgtactg aacatttctt tgttccatcg acggaaaaga cattccaccg tggtagcaca
     3001 gattattttg taaatgcccg taaaggcgat attggcgcgt tcgattcgcc gaaatttatc
     3061 ggcctgccgg taggcgaagt agtgaaagtg gcgaaagatc atctcgatgt tgccgttacc
     3121 gagccactgg caaatggcga tggcctgaac gtgttgatta aacgtgaagt cgtcggtttt
     3181 cgtgccaata cggtcgagaa aaccggagaa aatcagtacc gcgtctggcc caatgaaatg
     3241 ccagcagatt tgcacaaaat tcgcccgcat cacccactaa accgtaatct tgatcataac
     3301 tggcagcagg cactgacaaa aacctccagc gaacgtcggg tggcggtgga cattgaactg
     3361 ggcggttggc aggaacaatt gattctgact ctcaccagtg aagagggggt cagcataacg
     3421 catacgctgg atgggcagtt cgacgaagcc aataacgcag aaaaagcaat gaacaatctg
     3481 aaggatggtc tggcaaaact ggggcaaacc atctattacg cccgcgatgt gcaaattaat
     3541 ttgccggggg cgctgtttgt accaaacagt ctgttaaacc agttccgccg tgaagctgct
     3601 gacatgctgg atgctgcgcg tcttgccagt taccagcgcg gcagccgtaa accggttgct
     3661 gatcctgcgc cggtttatcc gcaaacgcat ctgagtttcc tcgcgaacgt ttacaaccag
     3721 aaagcgcgtg aattttatca tcgctatggt gtgcagctga ttgacgcggc gtatgaggca
     3781 catgaagaga agggcgaagt cccggtgatg atcaccaagc attgtctgcg ctttgccttt
     3841 aatctgtgcc cgaaacaggc gaaaggcaat atcaaaagct ggaaggcgac gccaatgcaa
     3901 ctggtaaacg gcgatgaagt attaacgcta aagtttgatt gccgcccatg cgagatgcac
     3961 gtcattggca aaatcaaaaa tcacatactg aaaatgcctt taccgggaag cgtagtggca
     4021 tccgtaagtc cggatgagct gctgaaaaca ttgccgaagc gaaaagggta aaacgccagt
     4081 tttctggtta ctcaccactc attgaatctg catgatattg cctgccgggt aaggcgtcac
     4141 gccgcatccg gcatcaaacg actcaacgtt gtcccgcttc tggtttgcgc gatttttgcc
     4201 agtaaaaatg ctcgcgcaaa ccttccgccg actcttccgc cacagcacgc aattcatcgc
     4261 tgtccgcttc atgacgcagc tgatgatcca cattctttac ccacacaaat tcatgtcctt
     4321 tgtgccctgc catgagttgt cctgagaaca gcgcacacgt taataagaca accgataacg
     4381 ccttcgtaaa catcctgcca cctttttctt accttttgcc gctatgatgc cgatcgtttc
     4441 ttgaggttat tattcagttt tgcaaattag cgcaaagaaa ttctggaatc ttccttcctg
     4501 attttgcatt gcattctgcc gtcgcggcga tttagtgcta tttttgagta aattacacac
     4561 ggatgtaaac aataatgttt actttgttga tatactcagc ggcagggagg cgatgtgaaa
     4621 caaagcgagt tcagacgttg gctcgaatct cagggcgtcg atgtagcgaa tggcagcaac
     4681 catttgaaac tcaggtttca tgggaggcgc agtgtcatgc cgcgtcaccc ctgcgatgag
     4741 attaaagaac cattgcgtaa agcaatcctg aaacaactcg gtttgagtta atcgccaatt
     4801 aaaaaggtta atgacatgcg agggacagtc gaaattatgc gttatcccgt cactcttaca
     4861 cccgcgccgg aaggcggtta tatggtttct tttgtggata tccctgaagc gttgacgcag
     4921 ggcgaaactg tcgctgaagc gatggaagcg gcaaaagatg ctttactgac cgcatttgat
     4981 ttttattttg aagataacga gcttatccct ttaccttcgc cattaaatag tcatgatcac
     5041 tttattgaag tgcctttgag cgtcgcctct aaggtattgc tgttaaatgc ttttttacag
     5101 tcagaaatca ctcagcaaga gttagccagg cgaattggca aacctaaaca ggagattact
     5161 cgcctattta acttgcatca tgcgacaaaa atcgacgccg tccagctcgc ggcaaaggcg
     5221 cttggcaaag agttatcgct ggtgatggtt taattacagt taacgaaaag ttgtcatttt
     5281 taacaactga tatagactgc cgaatcatct gcacataatt acgattcgat aatgaaaaaa
     5341 taccagcagc ttgcagaaca attacgcgag cagattgcgt cgggtatctg gcaacccggc
     5401 gatcgtttgc cttcgttgcg tgaccaggtg gcgctttctg gcatgagctt tatgactgtc
     5461 agccatgcct atcagttgct cgaaagtcag ggatatatta tcgcacgacc gcagtcgggt
     5521 tattacgttg cgccacaggc aataaaaatg ccgaaagcgc cagtcattcc agtcactcga
     5581 gatgaagcag tcgatatcaa cacttatatt tttgatatgt tgcaggccag tcgcgatccg
     5641 tcggtcgttc cgtttgcctc ggcctttccc gacccgcgac ttttccccct ccaacaacta
     5701 aaccgctcgc tggcgcaggt aagcaaaacc gccacggcga tgagcgtgat tgaaaacttg
     5761 ccgccaggaa acgcagaact gcgtcaggct attgcccgtc gctatgcctt acagggcatc
     5821 accatttctc ctgatgaaat tgtcattact gccggggcgt tagaggcatt aaacctcagt
     5881 ttgcaagcgg taactgaacc gggcgattgg gtgatagtag agaatccttg tttctacggt
     5941 gcgttgcagg cgctggagcg gctacggctg aaggcgttat cggtggcgac ggatgttaaa
     6001 gaagggatag atcttcagac gctggaactg gcgttgcagg agtatccggt gaaagcgtgc
     6061 tggctgatga ctaatagcca gaatccactc ggatttacct taacgccgca aaaaaaagca
     6121 caactggtag cgttgctcaa tcaatacaac gtaacgctga ttgaagatga cgtttacagc
     6181 gaactttatt ttggacggga aaaaccgctg cctgcgaaag cgtgggatcg ccacgatggc
     6241 gttttgcatt gctcttcgtt ttcgaaatgt ctggtgcctg gttttcgtat tggttgggtc
     6301 gccgccggaa aacatgcacg taaaattcaa cgcttgcagt tgatgagtac gctttccacc
     6361 agctcaccga tgcagcttgc gctggtggat tacctttcca cgcgccgata cgacgcccat
     6421 cttcgtcgcc tgcgtcgcca gcttgcggaa cgtaaacaac gtgcctggca ggcactgctg
     6481 cgttatctgc ctgcggaagt gaaaattcat cataatgaca gcggttattt tctctggctg
     6541 gagcttcccg aaccgttaga tgccggcgaa ttaagccagg cggcactggc gcatcatatc
     6601 agtattgcgc cgggtaaaat gttttctacc ggtgaaaact ggtcacgttt tttccgtttt
     6661 aataccgcgt ggcagtgggg agagcgtgaa gaacaggcgg taaaacaatt aggcaaactt
     6721 attcaagaac ggctgtaata gcgtttaatt taattcctct tagattgggt aatatgaatt
     6781 tcgaatagca gtcatatttc ctaactcctt gactatactc cagaagataa ccttacagac
     6841 ggcataatgc gcggtagctc acaacctgat taaattttct caggggcgaa ggtgtgcctg
     6901 caagccgccg tctatggtta aacaaggaga tatttttacg gcacggcggc tgaacaatta
     6961 attacgacag gagtaagacc ttatgagcaa gacatttgcc cgcagcagcc tgtgtgcact
     7021 cagcatgaca ataatgaccg ctcacgccgc cgaaccgcct accaatttag ataaaccgga
     7081 agggcgactg gatattatcg cctggccggg atacatcgaa cgcggacaaa ccgataaaca
     7141 atacgactgg gtgacgcaat ttgaaaaaga aacaggttgc gcggtgaatg tgaaaactgc
     7201 ggcgacttcc gatgaaatgg tcagtctgat gaccaaaggg ggttacgatc tggttacggc
     7261 atccggcgat gcctcgctgc gtttgattat gggcaaacgc gtgcagccga ttaataccgc
     7321 cttgattccc aactggaaaa cgctcgatcc gcgcgtggtt aaaggcgact ggtttaacgt
     7381 tggcggcaaa gtctatggca cgccatatca atggggtccg aacctgctga tgtacaacac
     7441 taaaaccttc ccgacgccgc cggatagctg gcaagtggtt tttgttgagc aaaatctgcc
     7501 ggacggcaag agcaataaag gccgcgttca ggcttatgat ggccctatct acattgcgga
     7561 cgctgcgttg ttcgttaaag ccactcagcc gcagttgggc atcagcgatc cgtatcaact
     7621 caccgaagaa cagtaccagg cggtgctgaa agtgctgcgt gatcaacata gtttgatcca
     7681 tcgctactgg catgacacta ccgtgcaaat gagcgatttc aaaaacgagg gtgtggttgc
     7741 ttccagtgcc tggccctatc aggccaacgc cctgaaagcc gaaggccagc ctgtcgctac
     7801 agttttcccg aaagagggcg ttaccggctg ggcagacacc accatgctgc atagcgaagc
     7861 gaaacatccg gtttgcgcct acaaatggat gaactggtca ttaacgccaa aagtgcaggg
     7921 cgatgtggcg gcctggtttg gctcgttacc agtcgtgccg gaagggtgta aagccagttc
     7981 gttattaggc gagaaaggtt gtgaaacaaa cggttttaac tatttcgaca aaatcgcctt
     8041 ctggaaaacg cctatagcag aagggggcaa gtttgttccc tacagtcgct ggacgcagga
     8101 ttacattgcc attatgggcg gtcgctaact tcgctggggt gctttatgac gtacgcagtg
     8161 gagtttgaca acgtctcgcg gttgtacggt gacgtgcgcg cagtagatag cgtcagtatt
     8221 gcgataaaag atggtgagtt cttctctatg ctggggccgt ccggctccgg caaaaccacc
     8281 tgcctgcgcc tgattgctgg cttcgaacag ctttccggcg gggctatctc tatctttggt
     8341 aaacccgcca gcaatctgcc accgtgggag cgggacgtga atactgtctt tcaggactac
     8401 gcgctatttc cgcatatgtc gattcttgac aatgtcgcct atgggctgat ggtcaaaggc
     8461 gtgaataaaa agcagcggca cgcaatggcg caagaggcgc tggagaaagt ggcgttgggg
     8521 tttgtacatc aacgtaaacc gtcacaactt tctggtggtc agcgccagcg ggttgctatc
     8581 gccagagcat tggtgaatga accgcgcgta ttgctgttgg atgaaccgct cggcgcactg
     8641 gatctcaaat tgcgtgagca gatgcagctg gaactgaaaa aactgcaaca gtctctcggt
     8701 atcactttta tcttcgttac ccacgatcag ggcgaagcgt tatcgatgtc cgatcgtgtg
     8761 gcggttttca ataatggacg cattgagcag gtcgattccc cgcgcgatct ctatatgcgc
     8821 ccgcgcacgc cgtttgttgc cgggttcgtt ggtacatcga atgtttttga tggactgatg
     8881 gcagagaaac tttgtggcat gacgggaagc ttcgccctgc gaccggaaca tatccgcctc
     8941 aacacccctg gtgaactgca ggccaatggc acgatccagg cggtgcaata tcagggcgcg
     9001 gcaactcgtt ttgaactgaa attgaacggc ggtgaaaaac tgcttgtgag tcaggccaat
     9061 atgacaggcg aagaactgcc tgccacgctc acgcccggac aacaggtgat ggtttcctgg
     9121 tcgcgtgatg tgatggtgcc gctggttgag gagaggtgaa tggcgatgaa tgtattgcaa
     9181 tcaccttcac gtccaggtct gggtaaggtg tccggtttct tctggcgtaa tccggggctg
     9241 gggctgtttt tactgctgct tggcccgcta atgtggtttg gcattgtcta tttcggctcg
     9301 ctgctgacac tgttatggca gggattttat acttttgacg atttcaccat gtcggtaacg
     9361 ccggaactga cgctggcgaa tatccgtgcg ctgtttaatc cggcgaatta cgacatcatt
     9421 ctccgcacgc tgactatggc tgtggcagtc actatcgcca gcgccattct ggcttttcca
     9481 atggcgtggt atatggcgcg ctataccagc gggaaaatga aagcgttttt ttatattgcg
     9541 gtaatgttgc cgatgtgggc gagctacatt gttaaagcct atgcctggac gttattgctg
     9601 gcaaaagatg gcgtggctca gtggttttta caacatcttg ggctggaacc actgctgact
     9661 gcgttcctta cattacctgc ggtgggcgga aatacgctgt caacttccgg gctggggcgc
     9721 tttctggtgt ttctctatat ctggttgccg ttcatgatcc tgcccgttca ggcggcgctt
     9781 gagcgtttgc cgccgtcatt gttgcaggcg tcggctgatc tcggcgcacg tccacgacaa
     9841 acctttcgct atgtggtgct gccgctggca atcccgggta ttgccgctgg ctctatcttt
     9901 accttctcac tcacactggg cgattttatc gtcccgcagc tggttggtcc tccaggatat
     9961 tttatcggca atatggttta ttcccagcag ggggcgattg gcaatatgcc gatggcggcg
    10021 gcattcaccc tggtgccgat tattctcatc gcactgtacc tggcgttcgt gaaacgtctg
    10081 ggagcgttcg atgcactctg aacgcgcacc gtttttcctc aaactggcgg cctggggcgg
    10141 cgttgttttc ctacattttc ccatcctgat aatcgccgcc tatgcgttta acactgaaga
    10201 tgcggcgttt agttttccac cgcagggcct gacgctgcgc tggtttagcg tggcagcaca
    10261 gcgtagtgat attcttgatg ccgtgacact gtcacttaaa gtggcggcgc tggcgacatt
    10321 aattgcgctg gtgttaggga cgctggcagc tgccgcgctg tggcgacgag actttttcgg
    10381 caaaaacgcc atttcgctgt tactgctgct gcccattgcg ctgccgggca ttgtcactgg
    10441 tctggcgtta ttaaccgcct ttaaaaccat caatctggag ccgggatttt tcaccatcgt
    10501 ggtcggtcat gcgacttttt gtgtagttgt ggtgtttaac aatgtcatcg cccgttttcg
    10561 ccgcacctcc tggagtctgg ttgaggcgtc aatggatctt ggggccaatg gctggcaaac
    10621 cttccgctac gtagtgttgc cgaatctcag ttcggcgtta ctggcaggag gaatgctggc
    10681 gtttgccttg tcgttcgatg aaatcatcgt tacgaccttt acggcaggtc atgaacgaac
    10741 gttaccgttg tggttgctca atcagcttgg gcgaccgcgt gatgtaccgg taactaacgt
    10801 ggtggcactg ctggttatgt tggtaacaac cttgccgatc ctgggggcct ggtggctaac
    10861 ccgcgaaggc gacaatggtc aataaccact gatacaggaa tatgctatgc aacataagtt
    10921 actgattaac ggagaactgg ttagcggcga aggggaaaaa cagcctgtct ataatccggc
    10981 aacgggggac gttttactgg aaattgccga ggcatccgca gagcaggtcg atgctgctgt
    11041 gcgcgcggca gatgcagcat ttgccgaatg ggggcaaacc acgccgaaag tgcgtgcgga
    11101 atgtctgctg aaactggctg atgttatcga agaaaatggt caggtttttg ccgaactgga
    11161 gtcccgtaat tgtggcaaac cgctgcatag tgcgttcaat gatgaaatcc cggcgattgt
    11221 cgatgttttt cgctttttcg cgggtgcggc gcgctgtctg aatggtctgg cggcaggtga
    11281 atatcttgaa ggtcatactt cgatgatccg tcgcgatccg ttgggggtcg tggcttctat
    11341 cgcaccgtgg aattatccgc tgatgatggc cgcgtggaaa cttgctccgg cgctggcggc
    11401 agggaactgc gtagtgctta aaccatcaga aattaccccg ctgaccgcgt tgaagttggc
    11461 agagctggcg aaagatatct tcccggcagg cgtgattaac atactgtttg gcagaggcaa
    11521 aacggtgggt gatccgctga ccggtcatcc caaagtgcgg atggtgtcgc tgacgggctc
    11581 tatcgccacc ggcgagcaca tcatcagcca taccgcgtcg tccattaagc gtactcatat
    11641 ggaacttggt ggcaaagcgc cagtgattgt ttttgatgat gcggatattg aagcagtggt
    11701 cgaaggtgta cgtacatttg gctattacaa tgctggacag gattgtactg cggcttgtcg
    11761 gatctacgcg caaaaaggca tttacgatac gctggtggaa aaactgggtg ctgcggtggc
    11821 aacgttaaaa tctggtgcgc cagatgacga gtctacggag cttggacctt taagctcgct
    11881 ggcgcatctc gaacgcgtca gcaaggcagt agaagaggcg aaagcgacag ggcacatcaa
    11941 agtgatcact ggcggtgaaa agcgcaaggg taatggctat tactatgcgc cgacgctgct
    12001 ggctggcgca ttacaggacg atgccatcgt gcaaaaagag gtatttggtc cagtagtgag
    12061 tgttacgccc ttcgacaacg aagaacaggt ggtgaactgg gcgaatgaca gccagtacgg
    12121 acttgcatct tcggtatgga cgaaagatgt gggcagggcg catcgcgtca gcgcacggct
    12181 gcaatatggt tgtacctggg tcaataccca tttcatgctg gtaagtgaaa tgccgcacgg
    12241 tgggcagaaa ctttctggtt acggcaagga tatgtcactt tatgggctgg aggattacac
    12301 cgtcgtccgc cacgtcatgg ttaaacatta aggataatat tgcagatcgt aagagtatga
    12361 gatgatcttg cgtactgtca gagcactgat ttctctgaca gtacgtgctg cgtgcaaaat
    12421 cactacatca aataaaccag ccaaatctca tagagataag agtaaacaaa ccaacagcag
    12481 caaagacatt tatcagtact acagttctac tggaaacatt catttttgcc accttttatc
    12541 attgccccta tgtgtatagt acgggttaag aaaatccgca ataatactca ttctgaagat
    12601 ggataagggc aagttgctgt ttgatgattt tttctcaaac tggtgaaaaa acttgatgca
    12661 cgtcaaaaaa tgacgcatat ttgcgcgcgt tttattcatc tggctggacg cccgtacatg
    12721 tctctctatc aacacatgct tgttttttat gcggttatgg cagcaatcgc atttcttatc
    12781 acctggtttc tttctcacga taagaaacgc atccgtttct taagcgcctt tctggtgggg
    12841 gcaacatggc cgatgagttt cccggtggcg ctgttgtttt cactgtttta aatcttactc
    12901 actggcttga cggcaccacg cagacttata tcatttggat gaatcgataa atttcacaag
    12961 tggctaagga gaaagtatgt cgcatctgga tgaagtcatc gcgcgcgtgg atgccgcgat
    13021 tgaagagagc gtcattgccc atatgaacga attattaatc gccctgagcg atgacgcgga
    13081 gttaagtcgg gaagatcgct acacccagca gcaacgtctg cgcacagcga tcgcccatca
    13141 tggtcgcaag cataaagaag atatggaagc gcgccacgaa cagttaacca aaggcggcac
    13201 catcctctga ttaaaatgag cgtctggcta ccaaccaggc gccaaccaca agtaaaattg
    13261 cgccacaaat tgggccgaac aacgcccgta gtggtacgcc attactgcgg aagatatcca
    13321 gcatcagacc gccaatcagc tgactggcga ccagtacagc aatagttgtc gccgctccca
    13381 cattctgata tccgctgata ctggcgaaga caaaaaacga tcccagcaag ccaggtataa
    13441 gtgtccacca gcgcacgctg gacaccagtt cgccaaaccc cgccatgcct tgtttaaacc
    13501 ataaaataga aacaaagagc acaatcccca ccagtgagtt cagcaacatg gcgatgagaa
    13561 tggtagagga ggtctgggtg atgcgcacca tcagcgtgtt ctggacaacc agaccaattc
    13621 cggcggcaat caaaaaagca agggtgagcg actgattcat ccaatcgcgt ccggttcagt
    13681 gcgctcgtcg agttgcaact gcataaatgt cagatccagc caacgaccaa atttagtgcc
    13741 tacctgcggc atttgcgcgg tgacgacaaa tcccagcgac tggtggagat gcagcgaggc
    13801 ctgattttgc gattcgatcc cggcgaccat gacatgcttc ccgcaatccc gcgcttcatc
    13861 aatcaatcgg cttaacaatt tacgccccag acctttgccc tgatgatcgg gatggacata
    13921 aaccgaatgt tccacggtat ggcgaaaacc atcgaaacta cgccagtcgc caaacgaggc
    13981 atatcccgtc actacgccat cttcttcgct caccagcacc ggataaccag ctaaagtccg
    14041 cgcttcaaac caggcaatgc ggttatctgc atccaccgtt tggtcattcc agatagccgc
    14101 cgtatacaac acggcgtggt tataaatttc cgcaatagca gcacaatcgg ctttgcgggc
    14161 aaaacggatg gacatgtctg gcctcgataa aataatgatg atgatgttat caatactatc
    14221 gataacagcc cgaagcaacg gattcctcga tgaaattatt gcgtgttcag aggggatgtg
    14281 acggctgcaa acaaaaccaa tcccgtgagc ctgctactat tgatggattc atgccaaaag
    14341 cacaggagag cattatgggg caacaaaagc agcgtaatcg acgttgggtt ctggcctcgc
    14401 gtccacatgg cgcaccggtt ccggagaatt tccgtcttga agaagatgat gtcgccacac
    14461 cgggtgaagg acaggtgtta ctgcgcacag tttatttgtc cctggacccg tatatgcgtg
    14521 gacgtatgag cgatgagcca tcttattcac cgcctgttga tattggcggc gtgatggtcg
    14581 gcggcacggt gagccgtgtt gtggagtcga atcatcctga ttatcagcct ggcgactggg
    14641 tgctgggcta cagtggatgg caggactatg acatatccag tggtgatgat ctggtgaaac
    14701 ttggcgatca tccgcaaaat ccatcgtggt cgctgggtgt gctggggatg ccaggcttta
    14761 ccgcttatat ggggctgctg gatatcggtc agcctaaaga gggcgaaacg ttggtggtag
    14821 ctgcggcgac aggaccagtg ggggcgacgg tggggcaaat cggcaaactt aaaggttgca
    14881 gagtggtggg ggtagccggt ggcgcggaaa aatgccgcca tgctaccgag gtgttaggct
    14941 tcgatgtttg tcttgatcac cacgcggatg attttgccga acaactggcg aaagcgtgcc
    15001 caaaaggtat tgatatctat tatgaaaacg tgggcggtaa ggtattcgat gcggtgctac
    15061 cgttacttaa tacatctgcg cgcattcccg tctgcggatt agtgagcagc tataacgcta
    15121 cagagctacc acccggtccg gatcgtttac ccctgttgat ggcaacggtg ctgaaaaaac
    15181 gtattcgctt gcaagggttt attatcgctc aggattatgg tcaccgcatc catgagtttc
    15241 agaaggagat ggggcaatgg gtgaaagagg ataaaatcca ctaccgcgaa gatattactg
    15301 atggtttaga gaatgcgcca cagacgttta tcggcctgct gaagggtaaa aacttcggca
    15361 aagtggtgat ccgcgtggcg ggtgatgatt aaagtttcaa cggcggcgta agccgccata
    15421 aaaatccagg caaaaagctt attttcagct ttaattaact aaccacgtca ttgttctgct
    15481 gctaataaca acaaaggtga gtggtttatg attattcact ttaatacacc aggtgaactc
    15541 cttctgccat gcaggcaagg ttggacagaa aacacgcgaa ggaattaaaa tgcctggaac
    15601 gggaaaaatg aaacatgtca gtttgactct gcaggttgag aacgacctga aacatcagct
    15661 tagtattggc gcattaaaac ctggcgcacg cctgattact aaaaatctgg cggagcaatt
    15721 aggtatgagt attacacctg tgcgtgaagc attattacgt ctggtttcgg tgaatgcgct
    15781 ttctgtcgca ccagcgcaag catttacagt tccggaagtg gggaaacgtc aattagacga
    15841 aatcaatcgg atccgctacg aactggaatt aatggcagtt gctctggctg ttgaaaacct
    15901 caccccgcaa gacctggcgg aactccagga attgctcgag aaattacagc aggcgcaaga
    15961 aaagggcgac atggaacaaa tcattaatgt aaacaggcta tttcgcttag cgatttatca
    16021 tcgctcaaat atgcccatcc tgtgtgagat gattgagcaa ctgtgggtca ggatgggacc
    16081 tggtttacat tatctctatg aagcgattaa tccagcggaa ttacgggagc atatagaaaa
    16141 ctatcatctg ttactcgctg cgttaaaagc aaaagacaaa gagggatgca gacattgtct
    16201 tgctgaaatt atgcaacaaa atattgctat tttataccag caatacaatc gttaaagtaa
    16261 atgcccggca aataccgggc atcaaggtaa ttactcaaat cgccatgcga tattcacgcc
    16321 gacaccataa tttcgcccgg gcgcaggttc gtaatatcgc ccgtttgact cattgacaat
    16381 gacagaacca acgtattctt tatcgaataa attatcgaca cgaccaaata aatccacagt
    16441 taaattgtgg taattgtatt tatacccggt gaataagccg atgagagtat aagacggcgc
    16501 cttggccgta ttttcatcat ctgccataat atcgcccata taacgcgctt ccgtgcctgc
    16561 ataccagcca tcttccggta cataacctat cgacgcaaag cccatattac gggcgatccc
    16621 aggcatccga ttaccgttac aatcctgttc attgcaaaca ttgctgcgat aggtcgcatc
    16681 aagccaggtc caggacgcgt ttacgcgaaa atctcccgcg aaacgttgat cccatgccag
    16741 ttcagcgcct tgacgacggg tctttccagc atttttgtaa gtcgtacgcc caccgctact
    16801 gctatcgaca acaatttcat catcggtgtc ggtctggaac aatgcgagac taagcagccc
    16861 atcaccaata cgcgttttac tgccgatctc aattgtatcg ttggtggatg gttttaaacc
    16921 aaagttcata ccgctttgcc catcagcccg ataagacagc tcattaatcg tcggcgtttc
    16981 aaaaccacgc ccggctgcca gatagatatt ccaggcatcg gtcattgcat attttaacga
    17041 tccggctggt agccatttgt gataactggc atcaccgctg tcatcgccgt tactcggagt
    17101 aatgtaatgg tcgttggaat caaaccacac ggagctgtag cgcacgccag catccagcga
    17161 cagtttttcg ctcagctgcc actgcgtctg taaataggga tcgatgttcc acatcagatt
    17221 gcgttcgtcg cgacgcaact cacctttttg cccgtactcc ggcatgccgc tattcaggcg
    17281 gaagttattg tagcccttgc ggttttcact catgttttcg taattcaggc cgatagtgaa
    17341 cgtgaccgga acgcccagtt caccacggtg tgtccagcgg ctgtctattc cctggtaatg
    17401 gcgttgcagg gtaatcacgc cgcccgcatg tgacgggtta agttgtggtg ccatgggtat
    17461 tgactggtac tgggtcgttt ctcgctctcc ggcatacatc atcacactca tatcatcccg
    17521 cgaactcagg ctacgttcat agcgcaaccc agcctgagtt tgcttgatgg tttttcgcgt
    17581 gtcgtactgt tctgcacgag gcgcttgttg tggattcgcc ttccattctg ctttggttag
    17641 cccacctggg tcatctgctt tgatatccac actattgaaa atcaggctta atttgctggc
    17701 ttcatcaatg cgtacgccca gtttggcatt ggctaaattt ttctgtgcgc cactatggtc
    17761 acgatagccg tgggtcgtaa aacgcgtggt tgagacggtg taatcaacat cgccgggttg
    17821 tgtgccgtct cccgttgcgc ccgttgcttt cagcccatag cgccagctgc caaaactgcc
    17881 gtagtaacta ctggcttcaa tggttggtgg ctgttgtccg gtctgggtgg tgacattcat
    17941 tacaccgcca gacgcgttgc catacagggc agaaaagggg ccacgcagca cttccacatt
    18001 ttgcacactg cttaaatcga tgttggatgt ttgcccttgc ccgtcgggca tggtggcggg
    18061 aataccgtcc acatacaggc gaataccgcg aattccgtaa gtggagcggg agccaaatcc
    18121 gcgaatcgac agctgtaaat cttgcgcata gttctgccga ttttgtacct gcaaaccagg
    18181 cacgctggtc agtgattcgg acaagttaat gcgcggtgtt gccaggcgca tcccctcgcc
    18241 atccaccacg cttactgctg ctggggtatc cagttctgaa accacctgcg gtgcggcact
    18301 gacaatcatg gtttgttcat cagcggcaaa aacaacgggg gaaagggcaa gtagtgcggg
    18361 caaaacggtc tgtcggacgg aaaaaatctt catgaaaaaa gccaggttaa gaatgggaaa
    18421 acgccgttat ggtaatgaaa ttgtaaattt atggaaaacg aaacggcacg atacgttaag
    18481 taattgagaa aattgtagtc gtaacggcaa gaaatgctcc acatttgaga aaataatgat
    18541 taccattccc atttataaca agagcgtaac gatgattacg cttagcgaag cattgtgaag
    18601 cagcaaaaat atcggttcat caaagggagt cgtcatgcat ttacgtcatc tgttttcatc
    18661 gcgcctgcgt ggttcattac tgttaggttc attgcttgtt gcttcatcat tcagtacgca
    18721 ggccgcagaa gaaatgctgc gtaaagcggt aggtaaaggt gcctacgaaa tggcttatag
    18781 ccagcaagaa aacgcactgt ggctcgccac ttcgcaaagc cgcaaactgg ataaaggtgg
    18841 cgtggtttat cgtcttgatc cggtcactct ggaagtgacg caggcgatcc ataacgatct
    18901 caagccgttt ggtgccacca tcaataacac gactcagacg ttgtggtttg gtaacaccgt
    18961 aaacagcgcg gtcacggcga tagatgccaa aacgggcgaa gtgaaaggcc gtctggtgct
    19021 ggatgatcgt aagcgcacgg aagaggtgcg cccgctgcaa ccgcgtgagc tggtagctga
    19081 tgatgccacg aacaccgttt acatcagtgg tattggtaaa gagagcgtga tttgggtcgt
    19141 tgatggcgag aatatcaaac tgaaaaccgc catccagaac accggtaaaa tgagtaccgg
    19201 tctggcgctg gatagcaaag gcaaacgtct ttacaccact aacgctgacg gcgaattgat
    19261 taccatcgac accgccgaca ataaaatcct cagccgtaaa aagctgctgg atgacggcaa
    19321 agagcacttc tttatcaaca ttagccttga taccgccagg cagcgtgcat ttatcaccga
    19381 ttctaaagcc gcagaagtgt tagtggtcga tacccgtaat ggcaatattc tggcgaaggt
    19441 tgcggcacca gaatcactgg ctgtgctgtt taacccagcg cgtaatgaag cctacgtaac
    19501 gcatcgtcag gcaggtaaag tcagtgtgat tgacgcgaaa agctataaag tggtgaaaac
    19561 gttcgatacg ccgactcatc caaacagcct ggcgctgtct gccgatggca aaacgctgta
    19621 tgtcagtgtg aaacaaaaat ccactaaaca gcaggaagct acccagccgg acgatgtgat
    19681 tcgtattgcg ctgtaataga tgtggcgagg gtgaaaacgg agagacttgc ctgactttta
    19741 tcacgccgca tgcggcacac cttcgtcagt aatctaaatt tgcccggcac gtagccgggc
    19801 aattttgcaa ttagctcgcc gtttcaggct taaacacaat ttcctgtttt tcttcatctt
    19861 cctcgacgac tggcgcagtg ctgtgaattt cagcaacgcg tttgcgcaca ccaaaccagc
    19921 ctataaccag cagaataccg ataatcggca gcgccgcgat agtataagtc ccgttcgggt
    19981 aatcgaacgc catcagcacc aggacactca gtaaaaacag taatgtcagc caggaagtga
    20041 agggcgcgcc aggcagttta aaactgacat ccgctgcttt gccttgttta attgctttac
    20101 gcaggcgcat ctggcacacg atgataaacg cccatgaagc gatgattccc agcgacgcga
    20161 agttcaacac aatctcaaat acgcgcgacg gcaccagata gttgaggaat acgccgacga
    20221 catacacaac tagtgtcgcc agaatcccgg catacggcac atgctgacga ctcattttcg
    20281 ccataaaact cggtgcggaa ccgcccatcg ccattgagcg cagaatacgt ccggtgcagt
    20341 acagacctga gttcaggctg gagagggcag cggtcagcac cacaatgttc ataatgctgc
    20401 cgatatatgg cacacccagt ttagagaaaa acgtcacgaa cggactttgc cccgcctgat
    20461 acgcgctcca cggcaataac ataaccagca acaccacgga gccgacgtaa aacaggccaa
    20521 tacgccaaat cacactgtta atggctttag gcaccatggt ctgcggatct ttacattctc
    20581 cggcagctgt acccaccatt tcaatggagg caaaagcaaa cactacgccc tgaatcaaca
    20641 ccagcgcagg cagcaaaccg tgggggaaga agccgccatt atcggtgatt aaatgaaagc
    20701 cagtggtgtt gccatccagc ggctgaccac taccgaggaa cactgtaccc acgaccaaaa
    20761 aggtcacaat ggcgagcact ttaataagcg caaaccagaa ctccatctcc gcaaaccatt
    20821 tcacaccgat catattcatg gtgccaacga tggtaagtgc agcgagcgca aagacccact
    20881 gcggcacgcc gccaaacgca ccccagtaat gcatatacag agcgacggcg gtaatatcaa
    20941 caatccccgt catcgcccag ttgatgaagt acatccagcc agcaacataa gcggctttct
    21001 cacccaaaaa ctcacgggca taagagacaa aactgccact ggaagggcgg tgtagcacca
    21061 gctcacccaa tgcacgcaga ataaaaaacg aaaacaagcc acaaattaaa taaaccagtg
    21121 ccagtgctgg acccgccatt tgcagtcggg ctcctgcacc taaaaacaag ccggtgccaa
    21181 tcgcgccgcc aatggcgatc atctgtacct ggcgattgcc catcgctttg tgatacccct
    21241 cttcgtgggc attaagccag cggcgtttcg cggcgtgttg atctgaagtg tcggtgtcgt
    21301 gtttactcat tgctctccct gattgcttta atgaaaaagt catataagtt gccatgaaca
    21361 atgtttattc atcctggcga tgatagttat caccattatt ctttatgggt ataactgtcg
    21421 gcgcagaatc ctacccgtta tcgataaacg atgcaaaaca tccccttaca atcctgaagg
    21481 ggaataatac aactgacgaa aaaatgacaa atctttttgc tggttaacct gtgtactgtc
    21541 ctacacttaa tctttaaaag attgtgaggg catatgatta agatgtatgg cgtacccgga
    21601 tggggctcta caatcagtga gctgatgcta acactggctg atattcctta tcagttcgtt
    21661 gatgtcagtg gttttgacca cgagggagct tcacgcgagt tattgaaaac cctgaacccg
    21721 ttgtgtcagg ttccgacact ggcgctggaa aatgacgaga ttatgacgga gacagcggcg
    21781 attgcattga tggttctcga tcgtcgcccg gaccttgcgc caccggttgg gcgcgccgaa
    21841 cgtcagcagt ttcaacggct attggtctgg ctggttgcta atgtctatcc aacattcact
    21901 ttcgccgatt accctgagcg ttgggctcct gacgcacctg aacagttaaa gaagaatgtt
    21961 attgaatatc gtaaatcgct ttatatctgg ctgaattcac aattgaccgc tgaaccttat
    22021 gcgtttggtg agcaactaac gctggtgga
//
LOCUS       DAOFQD010000073        22032 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.073, whole genome shotgun sequence.
ACCESSION   DAOFQD010000073 DAOFQD010000000
VERSION     DAOFQD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.073"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(72..827)
                     /gene="mlaA"
                     /locus_tag="QBF46_002911"
     CDS             complement(72..827)
                     /gene="mlaA"
                     /locus_tag="QBF46_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDQ6675072.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1121..2053
                     /locus_tag="QBF46_002912"
     CDS             1121..2053
                     /locus_tag="QBF46_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDQ6675073.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2129..2203
                     /locus_tag="QBF46_002913"
     tRNA            2129..2203
                     /locus_tag="QBF46_002913"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2162..2164,aa:Arg,seq:cct)
     gene            complement(2644..3891)
                     /locus_tag="QBF46_002914"
     CDS             complement(2644..3891)
                     /locus_tag="QBF46_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HDQ6675074.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKNPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYKIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNTLLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3963..4877)
                     /locus_tag="QBF46_002915"
     CDS             complement(3963..4877)
                     /locus_tag="QBF46_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDQ6675075.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRSSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHSLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5093..6526
                     /gene="cscA"
                     /locus_tag="QBF46_002916"
     CDS             5093..6526
                     /gene="cscA"
                     /locus_tag="QBF46_002916"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDQ6675076.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(6534..7592)
                     /locus_tag="QBF46_002917"
     CDS             complement(6534..7592)
                     /locus_tag="QBF46_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ6675077.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            7760..7978
                     /locus_tag="QBF46_002918"
                     /pseudo
     CDS             7760..7978
                     /locus_tag="QBF46_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7996..9324
                     /gene="dsdA"
                     /locus_tag="QBF46_002919"
     CDS             7996..9324
                     /gene="dsdA"
                     /locus_tag="QBF46_002919"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDQ6675078.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(9432..10970)
                     /gene="emrY"
                     /locus_tag="QBF46_002920"
                     /pseudo
     CDS             complement(9432..10970)
                     /gene="emrY"
                     /locus_tag="QBF46_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
     gene            complement(10970..12133)
                     /gene="emrK"
                     /locus_tag="QBF46_002921"
     CDS             complement(10970..12133)
                     /gene="emrK"
                     /locus_tag="QBF46_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDQ6675079.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            12549..13163
                     /gene="evgA"
                     /locus_tag="QBF46_002922"
     CDS             12549..13163
                     /gene="evgA"
                     /locus_tag="QBF46_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDQ6675080.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            13168..16761
                     /gene="evgS"
                     /locus_tag="QBF46_002923"
     CDS             13168..16761
                     /gene="evgS"
                     /locus_tag="QBF46_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDQ6675081.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYHGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNDVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(16817..17962)
                     /gene="yfdE"
                     /locus_tag="QBF46_002924"
     CDS             complement(16817..17962)
                     /gene="yfdE"
                     /locus_tag="QBF46_002924"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDQ6675082.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(18036..18980)
                     /gene="yfdV"
                     /locus_tag="QBF46_002925"
     CDS             complement(18036..18980)
                     /gene="yfdV"
                     /locus_tag="QBF46_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDQ6675083.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(19050..20744)
                     /gene="oxc"
                     /locus_tag="QBF46_002926"
     CDS             complement(19050..20744)
                     /gene="oxc"
                     /locus_tag="QBF46_002926"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDQ6675084.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(20798..>22032)
                     /gene="frc"
                     /locus_tag="QBF46_002927"
     CDS             complement(20798..>22032)
                     /gene="frc"
                     /locus_tag="QBF46_002927"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDQ6675085.1"
                     /translation="GIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTRHQLR
                     DIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDHMGFT
                     WEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVSAAA
                     LGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDKLGYL
                     EEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWENTCK
                     AIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCAPVLS
                     MKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTAAVLQ
                     ELGYSDDEIAAMKQNHAI"
BASE COUNT         6340 a         5190 c         4806 g         5696 t
ORIGIN      
        1 ttttacttgt ggtaattgag ggataacaac aaaaaaggtg agttttgcga ctcacctttt
       61 ttatttgttt cttattcaga atcaatatct tttaaatcat cctgaatcgc ttgtgcgttc
      121 ggattttcct gcggtttgag ttcgccgcca ttagcgatga aatcatgacg ctggaagtac
      181 gcttcgcgca ccataatata aggatcggac gactgacgca gcagaccatc ggaatccaac
      241 aactgcgcac gagtttctat cccttcaagc gtccatttac ccacagacat cggccaggtc
      301 agccaggaaa gaaccgggta aagaccatcc gccatatcac caccgtcatc acgcagcgtg
      361 aagctaccgt agaacggtaa ctggacgtaa ggcccataac ccacgccata atgaccaagc
      421 gtactaccga agcggtgagg ttcagtccgt tgcagtttcg ggttcgccat ccctgcaaca
      481 tcaataaaac cgcccatccc caaaatggtg ttcaggaaaa agcgggtaaa gtggaccatc
      541 ccctgataag ggtcgccctg caagaagtag ttaaccatca ccgcaggttc ttcaaggttg
      601 ccagtaaagt tgctcaaacc gttacgcgcc ggttgcggaa cataatcacg ccaggcgaca
      661 gcgaccggtc gaacaatata cgggtctaat acattgaagt tgaagttgta catggtgcgg
      721 ttgaaccctt ctaacgggtc agaacgccct tgctgatctg tgccggaact cgcacacccc
      781 accagaagcg tagttcccag agcaagcgcc gacaggcgaa gcttcataaa tgtctccctg
      841 tttttttatg gcttatgcag tttgccatcc atgacggaac gataccgtat ccgcctgttt
      901 aggtgtgggc gattgtaaca gcacgtcaac tgatgtccag acgccctgat ttgctgattt
      961 gatcatagcc tggtaatcgc cgccctgtag gctacttgat tctatagaaa cagaaaaagg
     1021 caaacgcagc cttttctaca ttttcagagt aactcccgcc agttgcgagc aaaaaagccg
     1081 ctacgcttta gctatacgtg ctaattcaag agaagagacc atggacaacg acaaaattga
     1141 tcaacacagc gacgaaattg aagttgagag cgaagaaaaa gagcgcggca aaaaaataga
     1201 aatagatgaa gaccgactcc cctcccgggc gatggcaatt catgagcata tccgccagga
     1261 tggtgaaaaa gagctggaac gcgacgcaat ggcgctactg tggtcagcca ttgcggcggg
     1321 tctgtcgatg ggcgcttcgt tactggcaaa agggatattt catgtcgaac tggaaggtgt
     1381 gccgggcagc ttcttactgg agaatctcgg ttataccttt ggttttatta tcgtcataat
     1441 ggcccgccag caattattta ccgaaaacac cgtgactgcg gtactacccg tcatgcaaaa
     1501 accgacaatg agcaacgttg gcttacttat gcggttatgg ggagtcgtgc tgctgggtaa
     1561 tattctcggg actggtattg ctgcgtgggc atttgaatat atgcctatct tcaatgaaga
     1621 aactcgcgat gcatttgtca aaatcggcat ggatgtgatg aagaacaccc ccagcgagat
     1681 gtttgccaac gcgatcattt ccggctggct gatcgccact atggtatgga tgtttcctgc
     1741 agcaggtgcg gcaaagattg tggtgattat attgatgacc tggcttattg ccctgggtga
     1801 caccacccat atcgtggtcg gttctgttga aatcctctat ctggtgttta acggtacgct
     1861 gcactggagc gatttcatct ggcccttcgc actacctact ttagcgggga acatctgcgg
     1921 cggcaccttt atcttcgcgt taatgagtca tgcacagatt cgtaacgaca tgagcaacaa
     1981 gcgtaaagca gaagcacgcc aaaaagcaga acgtgcggaa aacattaaga aaaatgataa
     2041 aaacccggca taaatggcga gggtttaagc aatcgagcgg cagcgtactt accccgcact
     2101 ccattagcgg gtatactcat gccgcattgt cctcttagtt aaatggatat aacgagcccc
     2161 tcctaagggc taattgcagg ttcgattcct gcaggggaca ccagataccc ttcaaacgaa
     2221 atctaccttc accccgtaaa agataggttt ggcagcacac ttgccttata tctactcatt
     2281 tttactgcaa caggttgaaa tctcagcact gtcagaaagc gctgatgact aaacagccct
     2341 gggccgggcg atgtaaccat cacacagaat cctgatagcg aaatatggcg tgactcgata
     2401 cttcactccg caatgcattc cttgatgaat tcgcaggacc gtgatacacg ggacaggtcg
     2461 ctgaatgacg acaatgtcct ggaaatcagc gaaccgcgca tctgaagtac atttgagcga
     2521 ctgtaccaga acatgaatga ggcgtttaga ttaggtgatt attagcaggg ctaagcactt
     2581 tagtattatt attttccggt tgagggatag ggagatatcg acaacaaccg gaaaagttta
     2641 cgtctatatt gctgaaggta caggcgtttc cataactatt tgctcgcgtt ttttactcag
     2701 gaagaaaatg ccaaatagca acatcaggca gacaataccc gaaattgcga agaaaactgt
     2761 ctggtagcct gcgtggtcaa agagtatccc agtcggcgtt gaaagcagca caatcccaag
     2821 cgaactggca atttgaaaac caatcagaaa gatcgtcgac gacaggcgct tatcaaaatt
     2881 tgccacgctg tatttgaaga cggatatgac acaaagtgga acctcaatgg catgtaacag
     2941 cttcactaat gaaataatcc aggggttaac gaacagcgcg caggaaagga tacgcaacgc
     3001 cataatcaca acaccgataa gtaatgtatt ttttggccct acccgattca caaagaaagg
     3061 aataatcgcc atgcatagcg cttcgagtac cacctggaat gagttgagat aaccatacag
     3121 gcgcgttcct acatcgtgtg attcgaataa acctgcataa aagacaggaa aaagttgttg
     3181 atcaaaaatt ttatagaaag accacgtccc cacaataaat atgacgaaaa cccagaagtt
     3241 tcgatccttg aaaactgcga taaaatcctc tttttttacc cctcccgcat ccgccgctac
     3301 gcactggtga tccttatctt taaaacacat gttgatcatc ataaatacag cgccaaatag
     3361 cgagaccaac cagaagttga tatggggact gatactaaaa aatatgccgg caaagaacgc
     3421 gccaatagca tagccaaaag atccccaggc gcgcgctgtt ccatattcga aatgaaaatt
     3481 tcgcgccatt ttttcggtga aactgtcaag caaaccgcat cccgccagat accccaggcc
     3541 aaaaaagagc gcccccagaa ttagacctac agaaaaattg ctttgcagta acggttcata
     3601 aacgtaaatc ataaacggtc cggtcaagac caggatgaaa ctcatacacc agatgagcgg
     3661 attcttcaga ccgagtttat cctgaacgat gccgtagaac atcataaata gaatgctggt
     3721 aaactggttg accgaataaa gtgtacctaa ttccgtccct gtcaacccta gatgtccttt
     3781 cagccaaata gcgtataacg accaccacag cgaccaggaa ataaaaaaga gaaatgagta
     3841 actggatgca aaacgatagt acgcatttct gaatggaata ttcagtgcca taattaccta
     3901 cctgtcgtta aaaaattcac gtcctattta gagataagag cgacttcgcc gtttacttct
     3961 cactattcca gttcttgtcg acatggcagc gctgtcattg cccctttcgc cgttactgca
     4021 agcgctccgc aacgttgagc gagatcgata attcgtcgca tttctctctc atctgtagat
     4081 aatcccgtag aggacagacc tgtgagtaac ccggcaacga atgcatctcc cgcccccgtg
     4141 ctatcgacac aattcacaga cattccagca aaatggtgaa cttgtcctcg ataacagacc
     4201 accacacctt ctgcaccttt agtcaccaac agcatggcga tctcatactc ttttgccagg
     4261 gcgcatatat cccgatcgtt ctgtgttttt ccactgataa gtcgccattc ttcttccgag
     4321 agcttgacga catccgccag ttgtagcgcc tgccgcaaac acaagcggag cgaatgctcg
     4381 tcttgccata gatcttcacg aatattagga tcgaagctga caaaacctcc ggcatgccgg
     4441 atcgccgtca tcgcagtaaa tgcgctggaa cgcgaaggct cggcagacaa cgcaattgaa
     4501 cagagatgta accattcgcc atgtcgccag cagggcaagt ctgtcgtctc taaaaaaaga
     4561 tcggcactgg ggcggaccat aaacgtaaat gaacgttccc cttgatcgtt cagatcgaca
     4621 agcaccgtgg atgtccggtg ccattcatct tgcttcagat acgtgatatc gactccctca
     4681 gttagcagcg ttctttgcat taacgcacca aaaggatcat cccccacccg acctataaac
     4741 ccacttgttc cgcctaatct ggcgattccc accgcaacgt tagctggcgc gccgccagga
     4801 caaggcagta gccgcccgtc tgattctggc aagagatcta cgaccgcatc ccctaaaacc
     4861 catactttgg ctgacatttt tttcccttaa attcatctga gttacgcata gtgataaacc
     4921 tctttttcgc aaaatcgtca tggatttact aaaacatgca tattcgatca caaaacgtca
     4981 tagttaacgt taacatttgt gatattcatc gcatttatga aagtaaggga ctttattttt
     5041 ataaaagtta acgttaacaa ttcaccaaat ttgcttaacc aggatgatta aaatgacgca
     5101 atctcgattg catgcggcgc aaaacgccct agcaaaactt catgagcacc ggggtaacac
     5161 tttctatccc cattttcacc tcgcgcctcc tgccgggtgg atgaacgatc caaacggcct
     5221 gatctggttt aacgatcgtt atcacgcgtt ttatcaacat cacccgatga gcgaacactg
     5281 ggggccaatg cactggggac atgccaccag cgacgatatg atccactggc agcatgagcc
     5341 tattgcgcta gcgccaggag acgataatga caaagacggg tgtttttcag gtagtgctgt
     5401 cgatgacaat ggtgtcctct cacttatcta caccggacac gtctggctcg atggtgcagg
     5461 taatgacgat gcaattcgcg aagtacaatg tctggctacc agtcgggatg gtattcattt
     5521 cgagaaacag ggtgtgatcc tcactccacc agaaggaatc atgcacttcc gcgatcctaa
     5581 agtgtggcgt gaagccgaca catggtggat ggtagtcggg gcgaaagatc caggcaacac
     5641 ggggcagatc ctgctttatc gcggcagttc attgcgtgaa tggaccttcg atcgcgtact
     5701 ggcccacgct gatgcgggtg aaagctatat gtgggaatgt ccggactttt tcagccttgg
     5761 cgatcagcat tatctgatgt tttccccgca gggaatgaat gccgagggat acagttaccg
     5821 aaatcgcttt caaagtggcg taatacccgg aatgtggtcg ccaggacgac tttttgcaca
     5881 atccgggcat tttactgaac ttgataacgg gcatgacttt tatgcaccac aaagcttttt
     5941 agcgaaggat ggtcggcgta ttgttatcgg ctggatggat atgtgggaat cgccaatgcc
     6001 ctcaaaacgt gaaggatggg caggctgcat gacgctggcg cgcgagctat cagagagcaa
     6061 tggcaaactt ctacaacgcc cggtacacga agctgagtcg ttacgccagc agcatcaatc
     6121 tgtctctccc cgcacaatca gcaataaata tgttttgcag gaaaacgcgc aagcagttga
     6181 gattcagttg cagtgggcgc tgaagaacag tgatgccgaa cattacggat tacagctcgg
     6241 cactggaatg cggctgtata ttgataacca atctgagcga cttgttttgt ggcggtatta
     6301 cccacacgag aatttagacg gctaccgtag tattcccctc ccgcagcgtg acacgctcgc
     6361 cctaaggata tttatcgata catcatccgt ggaagtattt attaacgacg gggaagcggt
     6421 gatgagtagt cgaatctatc cgcagccaga agaacgggaa ctgtcgcttt atgcctccca
     6481 cggagtggct gtgctgcaac atggagcact ctggctactg ggttaacata atatcaggtg
     6541 gaacaacgga tcaacagcgg gcaagggatc cgcgtcactc ttcccccttc acgaccttca
     6601 ataatatgca atgcagcttc ccgcccgata atgtcatgtg gaagctgaat tgtggtcagc
     6661 ggcggtaaaa acagatgccc gacgccaacc agattatcaa agcccattac ggcgacatcc
     6721 tgcgggatac gtaccccctt cgccaggaga acctgataag cgacaaaggc tgcgcgatcg
     6781 ttaccacata tcagaacatc aaaatctggt ttgcccgatt tgaagtgggc attgagtaaa
     6841 cttgcgagat cggtgtagtg atcatcacct gttgccatgt gaaattgttt cacctcagcc
     6901 agatctcgtc cagcatcacg ccaggcctgc tcaaatccct gccgacgata ccctgttgcc
     6961 aacgcacttt ccggtagcca gaagcataac ggttgacgat agcccgccgc gagcaaatgc
     7021 tgtgttgatt catattgtgc agtgtaatca tcagggatat aactgggtaa cgctgggtca
     7081 tccgccacac agttcgccaa tacaatattt tcaccataca gagactcagg cagcgtgata
     7141 tgtcgcagcc ccattgtagt atagataatg ccatccggac ggtgggcaag cagctgacgt
     7201 gccgcgcggg cagcgtcatc ttcagaaaaa atattgatta aaaaactatt ccagccgaac
     7261 tcgctggcgg tttgctcaat ggcaagcaga atatcaacag agaaaggagt ggtagccgtg
     7321 tcctgcgcca gcacggcgag agtcgacggc ttacgtcctt gagcgcgcat cttacgggcg
     7381 gaaagatcag gaacataatt cagggtctgg attgcctgca atacgcggtc acgcgttgca
     7441 ggacgcacag attctgcatt atgcatcacc cgggagactg tcatcatcga cactcccgcc
     7501 aggcgtgcga catcctttaa tgaagccata cccaagccgt ttgccgtaaa acgggcactg
     7561 tagcagaaac agacgtcact ggcgagatcc aacgccctat cacctgacac agcaatacaa
     7621 taaaaaataa caataattcc cggacaattg tccccaattc cgcctctgtt ctcgccaacg
     7681 agtaaccata taggcaagta agtaaaacat gccaactggc atcgccaacc tggagaagaa
     7741 aatataaata tggatcattg tgctgccgct gtatcccgac atcagcccgg aaattattgc
     7801 gattgctatc ggttcaggtg caattggctg cactatcgtt acggactcgc ttttctggct
     7861 agtgaagcaa tattgcggcg ctacgctcaa tgaaacattt aaatactata cgacagcgac
     7921 atttatcgct tcagtcgtcg ctctggcggg cacattcctg ctgtcattta tcatctaagc
     7981 gcaaagagac gtactatgga aaacgctaaa atgaactcgc tcatcgccca gtatccgttg
     8041 gtaaaggatc tggttgctct taaagaaacc acctggttta atcctggcac gacctcattg
     8101 gctgaaggtt taccttatgt tggcctgacc gaacaggatg ttcaggacgc ccatgcgcgc
     8161 ttatcccgtt ttgcacccta tctggcaaaa gcatttcctg aaactgctgc cactgggggg
     8221 attattgaat cagaactggt tgccattcca gctatgcaaa aacggctgga aaaagaatat
     8281 cagcaaccga tcagcgggca actgttactg aaaaaagata gccatttgcc catttccggc
     8341 tccataaaag cacgcggcgg gatttatgaa gtcctggcac acgcagaaaa actggctctg
     8401 gaagcggggt tgctgacgct tgatgatgac tacagcaaac tgctttctcc ggagtttaaa
     8461 cagttcttta gccaatacag cattgctgtg ggctcaaccg gaaatctggg gttatcaatc
     8521 ggcattatga gcgcccgcat tggctttaag gtgacagttc atatgtctgc tgatgcccgg
     8581 gcatggaaaa aagcgaaact gcgcagccat ggcgttacgg tcgtggaata tgagcaagat
     8641 tatggtgttg ccgtcgagga aggacgtaaa gcagcgcagt ctgacccgaa ctgtttcttt
     8701 attgatgacg aaaattcccg cacgttgttc cttgggtatt ccgtcgctgg ccagcgtctt
     8761 aaagcgcaat ttgcccagca aggccgtatc gtcgatgctg ataaccctct gtttgtctat
     8821 ctgccgtgtg gtgttggcgg tggtcctggt ggcgtcgcat tcgggcttaa actggcgttt
     8881 ggcgatcatg ttcactgctt ttttgccgaa ccaacgcact ccccttgtat gttgttaggc
     8941 gtccatacag gattacacga tcagatttct gttcaggata ttggtatcga caaccttacc
     9001 gcagcggatg gccttgcagt tggtcgcgca tcaggctttg tcgggcgggc aatggagcgt
     9061 ctgctggatg gcttctatac ccttagcgat caaaccatgt atgacatgct tggctggctg
     9121 gcgcaggaag aaggtattcg tcttgaacct tcggcactgg cgggtatggc cggacctcag
     9181 cgcgtgtgtg catcagtaag ttaccaacag atgcacggtt tcagcgcaga acaactgcgt
     9241 aataccactc atctggtgtg ggcgacggga ggtggaatgg tgccggaaga agagatgaat
     9301 caatatctgg caaaaggccg ttaataacgt ttcaacgcag catcgcaatc ctttccctgg
     9361 gtgagcgatg ctgccgatgg cgcagactta agatccccgg tcttacccgc tataaccccc
     9421 tccttttatc atcacccaac gcctttcgct gtaaacggcg gtttcgcaaa ccaaaccaaa
     9481 accgtaagta agataaaagc aatagccgcc atacggaaaa tctcatttgc agaaatagaa
     9541 agtgactgct gggttattta attattaatt tcattaagaa ctcctgaaag cgaaccataa
     9601 tatttatcca taatttgtga cgatgaatta aacacggggt taaattgatc gatggttgct
     9661 gtcaactgac tatggtgtaa cgattcgcgt cgtccccaca gcgtcattgt caacgacgta
     9721 ccaactgatc ctgacaaggt acgaaaaaaa ttactcatac tcgaggcatt ggcaaattta
     9781 ttatctggca agcctgaaaa cgaaatcgtt gttaagggta aaaagaaaca ggcaacggcg
     9841 aatccctgaa aaaactgcgg caaaatgatg cctgtaaaat caatcgttgg cataaatgtc
     9901 acagaacgcc agtaatagca aaccgcatac atcaaaaaac taaatgtcac taacaaccgc
     9961 atgtctattt tgttgccata acgtcctatc aaaggtgaaa ttaatagtgg catgatgccg
    10021 atgggcgcat aagcaagtcc ggcccatatc gcattatacc ccatcgtttc ctggagtaac
    10081 tgcggcataa ggacgatcgc tccagagtaa aataaatacg cgcatgtgat actcacaata
    10141 ccaatggtga agttacggga cttaaacaaa ctgagatcaa gaatcgggtt ctctgaggtc
    10201 gactcccaaa tgactaaaga gatcagagaa ataactgata ctactgttaa tattattatt
    10261 gtactcgagt tgaaccaatc cagatcgcgc cctttatcaa gcataatttg caagccacca
    10321 acaccgagca ctaacagggt cagtcctggt agattcattt tgaccggtga agtctcagtt
    10381 tctcttcctt taagtaaggt taagcataat gtcaggacga taatccccat agggacattg
    10441 attaaaaata tccaacccca gctaaagtta tcacaaatat aaccgcccaa tatcggccca
    10501 catatcggag cgataatcac ggtcattgac cataatgcca gagcaaatgt tcttttttct
    10561 ggcggataat tccttaataa taaactctgt gacagtggaa ttaacggccc cgccattaac
    10621 ccctgaacga ctctaaaaaa tatcagcaca tcaagattgg tcgataggct acacattaat
    10681 gaagacagcg aaaaaaaagt gactgaaagt aaaaataatc ttaattcgcc tattctttgt
    10741 gccaacctgc cagtaacagg gatcgcaatg gcatttgcta caccaaacga ggtgataacc
    10801 caggtgcctt cgtctgttga tgctcccaga aagccagata ttgtcggtat tgcgacgtta
    10861 gaaatagtgg aatccaacat ttgcataaat gtcgctaatg acaatgcaat agtgacgcac
    10921 cataacgtcc caccggttaa tggtgccgga gttgatttag tgattgccat taaagttgtc
    10981 cattatgcga aataatgttg ctaatttctt tttctatcgg actggtatcg ataactaaag
    11041 ccttactggt ataagccggc atggaggtca cggttgaagc cagctcaggc atctcggcaa
    11101 tgtcttcgtt cttcgtatca atagttgctg tcatcgataa accaatacgc aaggggtgtt
    11161 ccatgagttc ttttggatca agagaaactt caaccggtac acgctgaacg attttgatcc
    11221 agttccctgt cgcattttgt gcaggtaata aggagaacgc attgccggtt cccatattga
    11281 tccctgtcac ccgaccatga aacacaacat tttcaccata aagatcgctg ataatattga
    11341 ccgattgacc aatccgtaca tccgtgagtt gtgtttcttt aaagttggca ttaacccaca
    11401 tttgacgtgc cggtacgaca gccattaacg attgtccggg gctcactgtt tcgccgacct
    11461 gaacacttct ctgggcaata tagccggtaa ccggactctt aatatccgta cgtttaagcg
    11521 ccaaccaggc ttctttagtt gcatccgccg cttcaacgac ttgtggctga cggtttaatg
    11581 gtgtgttcat tactaaagct ttattcgctt tataagcctg gatagcggca ttcaatgccg
    11641 ctttgctact tattaacgta tctttggtat gctccagcgt ttcttttgaa ataaccccct
    11701 gcttcgctaa cggcactcga cggttataat cttctaaaga ttgttgatac tgaatacgtg
    11761 ctgaagcgac ttcggcacta tattgtttat cctgtaagta tagtttattc gtttgccgaa
    11821 caatatttgc cagattattt ttagctttat tgagtgcgat agtggcatca gttttatcca
    11881 gtgaaactaa aatgtcacct tgtcgaacgt agttcgtatc tttatgatta acgacagtga
    11941 cactacctga gacttgtgca gaaattggat ctgcattccc cgtgacatag gcgtcatctg
    12001 tactaatcat gtcttctaat tccattgacc aataggcata ggcacctgaa aacgcaataa
    12061 ataaaactac cgccaataaa gaaaagtatt ttcttctgtt agaatgtttt ttatttgaat
    12121 taatctgttc cactattatc tctcatttct catagatgaa atttatgaat tgaatgaaaa
    12181 gagcattcat tcttcatatc aaacacagtg catgtgttgc acattatccc aggaacgttg
    12241 gaggcctaat ttaaatgagg tgcaaaaaaa acaacagagg actaaaccgt ggcttttgca
    12301 atacaattct tacgcctgta ggattagtaa gaagacttat agtgccaact tgaaactata
    12361 aatcatcggt acaatccctg attttattgt tgacatttca tttatgccga ctatttatat
    12421 ggtatacttg tcgaattatc ttaaaggaag ctcagatttt cttattttta ttgagaaaat
    12481 gagatgacgc cttatgtctg tattactaca gggagaaggg agatgcttca ttgcaaaggg
    12541 aataatctat gaacgcaata attattgatg accatcctct tgctatcgca gcaattcgta
    12601 atttattgat caaaaacgat attgaaatct tagcggagtt gactgaaggg ggaagcgccg
    12661 ttcagcgggt ggaaacactt aagcctgata tcgtcatcat tgatgtcgat atccccggag
    12721 ttaacggtat ccaggtgtta gaaacgctga ggaagcgcca atatagcgga attattatta
    12781 ttgtctccgc taaaaatgac catttttacg ggaaacattg tgctgatgct ggcgctaatg
    12841 gattcgtgag taaaaaagaa ggcatgaaca atatcattgc ggctattgaa gctgcaaaaa
    12901 atggctactg ctatttcccc ttctctctca accggtttgt tggaagttta acgtccgacc
    12961 agcaaaaact cgactcctta tcgaaacaag aaattagtgt catgcggtat attcttgatg
    13021 gcaaggataa taatgacatt gctgaaaaaa tgttcatcag caacaaaact gtcagcactt
    13081 ataaaagtcg cctgatggaa aaattagaat gtaaatcact gatggatctt tacacattcg
    13141 cgcaacgtaa caaaatcggc taatcacatg aagtttttac cctatttttt tcttctctgt
    13201 tgtggtcttt ggtcgaccat aagtttcgca gacgaagatt acatcgaata tcatggcatc
    13261 agtagtaaca accgtgtcac acttgatcca gtacgtctga gcaacaagga attacgttgg
    13321 ttagcgagca aaaaaaatct tgtgattgca gtacacaagt cccaaacggc taccttgttg
    13381 cataccgatt cgcagcaaca ggttcgtggt attaatgctg attatttaaa tcttttaaaa
    13441 agagcgttaa atatcaaatt aacactccgg gaatacgcag atcatcaaaa agcaatggac
    13501 gcgcttgcag aaggtgaagt cgatatagtg ttatcacatt tagttacttc gccgcctctt
    13561 aataatgaca ttgctgcaac caaaccattg ataattacct ttccggcgct ggtaaccacc
    13621 cttcacgact caatgcgacc gcttacctca ccaaaaccag taaatattgc tcgggtagca
    13681 aattaccccc cagacgaggt aattcatcaa tcatttccaa aagcaacaat tatctctttt
    13741 acaaatttat atcaggcatt agcatccgtc tcagctgggc acaatgatta ctttattggt
    13801 agtaacatca ttaccagcag tatgatttcc cgctatttca ctcactcctt aaatgtagtg
    13861 aaatattata actcgccgcg tcaatataat tttttcttga ccagaaaaga atctgtcatt
    13921 cttaatgatg tactcaatag atttgttgat gctttaacaa atgaagttcg ctatgaagta
    13981 tcacaaaatt ggcttgatac aggaaacctg gcctttctga acaaaccatt agaactcact
    14041 gaacatgaaa aacagtggat taagcagcat cccaatttaa aggtgctgga aaatccttac
    14101 tcgcccccct attctatgac ggatgaaaat ggctcggttc ggggcgttat gggggacatt
    14161 cttaatatta ttaccttgca aacaggttta aatttttctc cgatcaccgt ttcacacaat
    14221 atccatgctg gaacacagct tagccccgga ggatgggata taatacctgg cgctatttat
    14281 agtgaagatc gagaaaataa tgttttattt gctgaagcct tcataacaac gccttacgtt
    14341 tttgtcatgc aaaaagcgcc tgacagtgaa caaacattaa aaaaaggaat gaaagttgcc
    14401 attccatatt attatgagct gcattcgcaa ttaaaagaga tgtatccgga ggttgaatgg
    14461 atacaggtcg ataatgccag cgctgcattt cacaaggtta aggaaggtga acttgatgct
    14521 ctggtcgcga cacagttaaa ttcgcgttac atgatcgatc attactatcc taatgaactt
    14581 tatcattttc ttattcctgg cgttccgaat gcatcgcttt cgttcgcttt tcctcgcgga
    14641 gaaccggaac ttaaggatat tattaataaa gcactgaatg caattccccc aagcgaagtt
    14701 ctgcgcctga cggaaaaatg gattaaaatg cccaatgtga ccattgacac atgggaccta
    14761 tatagcgagc aattttatat tgttacgaca ttatccgttt tattagttgg cagtagcctt
    14821 ttatggggat tctacctgtt acgctcagtt cgtcgtcgta aagtcattca gggtgattta
    14881 gaaaaccaaa tatcattccg aaaagcactc tcggattcct taccgaatcc aacttatgtt
    14941 gtaaactggc aaggtaatgt cattagtcat aatagtgctt ttgaacatta tttcactgcg
    15001 gattactaca aaaatgcaat gttaccatta gaaaacagtg actcaccctt taaagatgtt
    15061 ttttctaatg cgcatgaagt cacagcagaa acgaaagaaa atcgaacaat atacacacag
    15121 gtatttgaaa ttgataatgg catcgagaaa agatgcatta atcactggca tacattatgc
    15181 aatcttcctg caagtgacaa tgcagtatat atttgtggtt ggcaagatat tactgaaacg
    15241 cgtgatctaa ttaatgcact cgaggtagaa aaaaataaag cgataaaggc tactgtagca
    15301 aaaagtcagt ttctggcaac gatgagtcac gaaataagaa caccaataag ctctattatg
    15361 ggcttcctgg aacttctgtc gggttctggt cttagcaagg agcaacgggt ggaggcgatt
    15421 tcacttgcct acgccaccgg acaatcactc ctcggcttaa ttggtgaaat ccttgatgtc
    15481 gacaaaattg aatcgggtaa ctatcaactt caaccacaat gggtcgatat ccctacttta
    15541 gtccagaaca cttgtcactc tttcggtgcg attgctgcaa gcaaatcgat cgcattaagt
    15601 tgcagcagta cgtttcctga acattacctg gttaagatcg accctcaggc gtttaagcag
    15661 gtcttatcaa atttactgag taatgctctc aaatttacca ccgagggggc agtaaaaatt
    15721 acgacctccc tggttcacat tgatgacaac cacgctgtta tcaaaatgac gattatggat
    15781 tctggaagtg gattatcgca ggaagaacaa caacaactgt ttaaacgcta cagccaaaca
    15841 agtgcaggtc gccagcaaac aggatctggc ttaggcttaa tgatctgcaa agaattaatt
    15901 aaaaatatgc agggcgattt gtcattagaa agtcatccag gcataggaac aacatttacg
    15961 atcacaatcc cggtagaaat tagccagcaa gtggcgactg tcgaggcaaa agcagaacaa
    16021 cccatcacac tacctgaaaa gttgagcata ttaatcgcgg atgatcatcc gaccaacagg
    16081 ctattactca aacgccagct aaatctatta ggatatgatg ttgatgaagc cactgatggt
    16141 gtgcaagcgc tacacaaagt cagtatgcaa cattatgatc tgcttattac tgacgttaat
    16201 atgccgaata tggatggttt tgagttgact cgcaaactcc gtgagcaaaa ttcttcctta
    16261 cccatctggg ggcttacagc caacgcacag gctaacgaac gtgaaaaagg gttaagttgc
    16321 ggcatgaact tatgtttgtt caaaccgttg accctggatg tactgaaaac acatttaagt
    16381 cagttacacc aagttgcgca tattgcacct cagtatcgcc accttgatat cgaagccctg
    16441 aaaaataata cggcgaacga tctacaactg atgcaggaga ttctcatgac tttccagcat
    16501 gaaacgcata aagatctacc cgctgcgttt caagcactag aagctggcga taacagaact
    16561 ttccatcagt gtattcatcg catccacggt gcggctaaca tcctgaattt gcaaaagttg
    16621 attaatatta gccatcagtt agaaataaca cctgtttcag atgacagtaa gcctgaaatt
    16681 cttcagttgc tgaactctgt aaaagaacac attgcagagc tggaccagga gattgctgtt
    16741 ttctgtcaga aaaatgacta aatagcggct cccacaatgt tcaaatgtgg gagctattta
    16801 ccagcacatc ttacatttat gatgagaact cctggcgaat ttgttcccca tgctggtcga
    16861 gcgttgccgc tcccggcata acatgcgggt ccgcgcagcc gctgattttt atcggattac
    16921 ccggcatcat tattcccccg gcttcaatca acatatttct cgcctgagtt tgtggcaatt
    16981 taatggcctc agccacactt aataacggcg cgacgggtac accaacttca tgtattctgg
    17041 ctaaccaaac ttcagctgcc tgcgttttta acgtccgctc aatatattgt ttaagaatag
    17101 cctggttttg tacgcgtaaa atattgctgc taaatcgggg atcattaacc agttccgtaa
    17161 gctccagtgc ctggcataac gcagaaaaaa gcttgtcatt accacaacaa atcgtaatcg
    17221 gcttatcctg agtattgaaa acatcaaaag gtgccatgta gggatggcga tttcccagac
    17281 gttgtggtga cttccctgtc gcgatatatg ccatcagacc atgctccaga aaactcagcg
    17341 tggcatcaaa catcgctata tcgacatgcg cccctctctg gctcttttca cggccataaa
    17401 gtgcactcac tattccgctg aataaataga caccgccgca tagatccgca agagatgtac
    17461 caacgcgcac tggcggagca tcaggatatc ctgtttccat cattatcccg ctcattgcct
    17521 gaatgatggt atcgtaggca ggagcatctt ttagcggacc ggtatgtccg aaacctgacg
    17581 atgaagcata tatgaggcgc gggttgattt cttgaagcgt ttcccatgaa aaccccagtt
    17641 tttccattgt acctgggcga aaattctcag ctaatacatc agcttgtttg agcatattta
    17701 taaatatact tttatcgtga tcattcttta aatcaagaac cacactctct ttgccatgat
    17761 taataaaact gtaatagagt gactgtccat ccacataggg accaaatgtg cgggtatcat
    17821 caccatgacc cggcggctca actttaatta cccttgcgcc catattacaa agaagttgag
    17881 ttccgaaagg tccattaagg acatgtgtca tatcgataac taataagcct tcaaacgggc
    17941 ctttgctttc attatttgtc atttgcttat gccttgaaaa aagccgaagg tgaaaagaca
    18001 cttcggcatt tatgtaaaat caataacatg attaattatg aaaccagtcg actgacataa
    18061 atccacaagg gagccgtgac gacaaagccc agtacgctca cagccaatga cgctgtacca
    18121 gtacgggtgt agacattaaa ccggctggca ataatgattc cggagaatgc cggcggtaat
    18181 gcgcctgcca gtaccatcat ctgcagatgt tcgctgttca aatgacatgc cataccaacg
    18241 agaagcagtg ccagtggcat cagaatcagc ttcaggaagg tgttataagc aatttcggca
    18301 ctgaactcga atttatgtgc agccagagtc aaccccgcag cgaataccgc tacccctgag
    18361 ttagccttcg caatcagatt aaaggttggg tcccatgctg ccggaatttt taccccaacc
    18421 aacaccagga tcgttgccag aacaggtgcc catactactg gctcctttgc cgcagaaatt
    18481 aatgcgctca gattactatt cttcttacca tccgctcctg aagaaggatt cagcaaatac
    18541 agaccaatag gaatagtaat tgcgttaaca ataatagaaa taattgctac cactaaacct
    18601 gttgatacgg aatcaccata aataggatcg agaactgcaa accccaggaa tccaatggta
    18661 ggtgaacctg caattaatgc acatacagct gcttctgcat gggtacgttt aaaaaatttg
    18721 tagcaaccga accaggagaa aaagaaacat ccgacaataa ccacaagtga tacaagggtc
    18781 agacgagtgt ccgcaaaaat catttccctg tttgcccgag taatagatac aaatagagcc
    18841 gcaggaagcg cgtagtttaa taccagttta ttaaatgccc gagcttgatc ttctgaaaat
    18901 gtttctcgtc tgccgctaaa ataacccaac agcatgatta cgataatagg caataaatcg
    18961 ccaataaaaa atgttagcat aatagtattt ccgataaggg tcccctcacc atcgcataat
    19021 gagttggcaa gaggagacga tgtcagtatt taattaccag cgacttgttt tgggttaagt
    19081 ttggtaatat ggccactttc agttcctgct gcagggtcga tgaccacatt aataatggtc
    19141 ggtttgcgcg actgaatacc ggtggttaaa gcatgacgaa gttcatctgt cgtggtgacg
    19201 ttatagccaa cgccacgaaa cgcatccatt aatttgtcat accttgcatg gtgcaacaga
    19261 tccgttggtg atggtgcacc agcgccactg agatcaacac cgtctcctct gtagatgccg
    19321 ccattattaa aaataacgat cgtcaccggc aggttatatc gacaaatcgt ttcaatttcc
    19381 atcccactga aaccaaaagc actatcacct tcaatggcga caaccggaga accagaagtc
    19441 acgctagcac cgatggcata gcccataccg atgcccatga caccccaggt accacaatcc
    19501 agacgacgac gtggtttata catatcaata atatttcgtg cattatccag ggtatttgca
    19561 ccttcattaa ctaaataaat atcctggttc tcgcgcaata cgtcgcgcac agcacttaat
    19621 gcattaaagt aatttaatgg ttgagtatct gtacttaatt tttcatgcat tttttgtgca
    19681 ttttgctgct tgtggatatt taaaatatcg cgccatacca gtggagtcgt aaatgtgttt
    19741 tgtttcagtt ctgccagcat accttgcata ctggatgcaa tatcaccaac gactggcaca
    19801 gcaatggggc ggttgctgtc aatttcctgc ggttcaatat ccagttgaat aaactgtgta
    19861 tctgccgccc atcctttttt accgtgtgcc aataaccaat tcagtcgtgc accaacaagc
    19921 atgacaacgt cagcatttgc cagggcaaac gaacgcgcag ctgccgcaga aagtggatgc
    19981 gtatcttcaa ggatcccttt cgccatagac attggcagga atggaatctg agcactttca
    20041 ataaattcac gaagctgttc atcagcttgt gaatacgccg cgcctttgcc aaggataatt
    20101 aatggccgtt cagcttttgc taaaagcgaa attgcgctag tgactgactt cgggcatggc
    20161 aataatgctg gcgacggatt ttcaacttta acaatcgtgg ttaacgcttc gtctttttcc
    20221 atcgtcgcgg ccaggacatt tgctggcaaa tcaagataaa ctccgccagg gcgacccgat
    20281 acagagaccc ggatagcgcg tgccaatgca atgccaagat cctgcggctg attaacgcga
    20341 aatgctgctt tggcatacgg ttttgccgca ttcatttggt ccagctcttc ataatcacct
    20401 tgctgtaggt cgacgatcgc gcggtcgctg gagccgctaa tcatgatcat cggaaaacca
    20461 tttaccgttg cgttggccaa tgcggtcaaa ccattgagga atcctggcgc agaaactgtc
    20521 aggcagatcc ccggtttttg ggtaagaaaa ccgcttgccg cagcggcata gcctgccgac
    20581 tgctcatgac gaaaaccaat ataacgaatg ccttccgcct gggcatggcg tgccatatcc
    20641 gtcacaggaa tacctacaac accataaata gtgtcaatat tattctgttt taatgcttca
    20701 acgatgatat gcataccatc tgtcatttga agttgatctg acatgattaa taacctcgaa
    20761 atttttataa attgggagcc agaagccccc gttaatatca gatggcgtgg ttttgcttca
    20821 ttgcagcaat ttcatcgtcg ctataaccca gctcctgcaa tacagcagcg gtatgttcac
    20881 ctaatagcgg cgcagcttta atatccggcg taaaggcaga gaatttcatt ggacaaccaa
    20941 cggtcagata ttttccacgc aacggttgtt ccacttcaac aacactgcca ctttggcgca
    21001 aagagggatc aagtgaaatt tctttcatac ttaaaaccgg tgcacaagga atatcaaact
    21061 gagtcaaata ggccaccgct tcatgtttat caatagtgac agtgtatttt tcgatttcag
    21121 caaaaatatc gaaaatatgt ggctgtcgtg catgggctgt actgtatgcc ggatcggtaa
    21181 tccattctgg tttgccgatg gctttacagg tgttttccca gttttgctcc tgaatagtga
    21241 aataaatata ggcgttagga tcggtttccc agcctttaca tttcaggatc cagccaggct
    21301 gaccgccacc acctgcatta ccaccgcggg gaactgcatc accaaatgta ccattcggat
    21361 actgcgggta ttcttccaga taacccaatt tatcgagacg ctgctggtca cgtaatttca
    21421 cgcggcaaag gttcaatacg gcatcctgca ttgacatggt gactcgttgc ccacgccccg
    21481 ttttttcgcg atgcagcaaa gcagcaagta aaccgatcag caaatgcatt ccggtgttgc
    21541 tgtcacccaa cgctgcagcg cttaccagcg gcggaccatc ccaaaaaccc gtagtggatg
    21601 ccgcgccacc cgctgcctga gcaacgtttt cataggcttt tacattcaca taaggcgaac
    21661 actcatcaaa ccctttgatc gaaccaaaaa tcagacgtgg attgatttct tgaatatgct
    21721 cccaggtgaa gcccatgtga tcaatggccc ctggatgaaa gttctcgact aagatatcag
    21781 cttcgcggat cagcttttcc attacctctt tgccttccgc tgttttggta tttaactcaa
    21841 tagaacgttt gttactgtta agcatggtga agtaaagcgc atcgatatca ggaatatctc
    21901 gcagctggtg acgcgttacg tcaccaacgc cgggacgttc aattttaata acgtcagcgc
    21961 caaaccaggc cagcatttga gtacaagatg ggccagattg cacaccggtg aaatcgagaa
    22021 ctttaattcc tt
//
LOCUS       DAOFQD010000074        21893 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.074, whole genome shotgun sequence.
ACCESSION   DAOFQD010000074 DAOFQD010000000
VERSION     DAOFQD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.074"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..144)
                     /locus_tag="QBF46_002928"
     CDS             complement(<1..144)
                     /locus_tag="QBF46_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDQ6675086.1"
                     /translation="MNKTLITATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLAN"
     gene            complement(427..1683)
                     /locus_tag="QBF46_002929"
                     /pseudo
     CDS             complement(427..1683)
                     /locus_tag="QBF46_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
     gene            1943..2863
                     /gene="cbpA"
                     /locus_tag="QBF46_002930"
     CDS             1943..2863
                     /gene="cbpA"
                     /locus_tag="QBF46_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDQ6675087.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            2863..3168
                     /gene="cbpM"
                     /locus_tag="QBF46_002931"
     CDS             2863..3168
                     /gene="cbpM"
                     /locus_tag="QBF46_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDQ6675088.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(3261..3860)
                     /gene="torD"
                     /locus_tag="QBF46_002932"
     CDS             complement(3261..3860)
                     /gene="torD"
                     /locus_tag="QBF46_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDQ6675089.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFAARCRQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(3857..6403)
                     /gene="torA"
                     /locus_tag="QBF46_002933"
     CDS             complement(3857..6403)
                     /gene="torA"
                     /locus_tag="QBF46_002933"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDQ6675090.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            complement(6403..7575)
                     /gene="torC"
                     /locus_tag="QBF46_002934"
     CDS             complement(6403..7575)
                     /gene="torC"
                     /locus_tag="QBF46_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDQ6675091.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7705..8397
                     /gene="torR"
                     /locus_tag="QBF46_002935"
     CDS             7705..8397
                     /gene="torR"
                     /locus_tag="QBF46_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDQ6675092.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(8370..9398)
                     /gene="torT"
                     /locus_tag="QBF46_002936"
     CDS             complement(8370..9398)
                     /gene="torT"
                     /locus_tag="QBF46_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDQ6675093.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            9481..12225
                     /gene="torS"
                     /locus_tag="QBF46_002937"
     CDS             9481..12225
                     /gene="torS"
                     /locus_tag="QBF46_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDQ6675094.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            12297..13370
                     /locus_tag="QBF46_002938"
     CDS             12297..13370
                     /locus_tag="QBF46_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDQ6675095.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHLGTATLISMTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(13418..13591)
                     /locus_tag="QBF46_002939"
     CDS             complement(13418..13591)
                     /locus_tag="QBF46_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDQ6675096.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(13581..13811)
                     /gene="ymcE"
                     /locus_tag="QBF46_002940"
     CDS             complement(13581..13811)
                     /gene="ymcE"
                     /locus_tag="QBF46_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDQ6675097.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            complement(13786..13974)
                     /locus_tag="QBF46_002941"
     CDS             complement(13786..13974)
                     /locus_tag="QBF46_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDQ6675098.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(13985..14197)
                     /gene="cspG"
                     /locus_tag="QBF46_002942"
     CDS             complement(13985..14197)
                     /gene="cspG"
                     /locus_tag="QBF46_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDQ6675099.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            14483..14695
                     /gene="cspH"
                     /locus_tag="QBF46_002943"
     CDS             14483..14695
                     /gene="cspH"
                     /locus_tag="QBF46_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDQ6675100.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            15137..15442
                     /locus_tag="QBF46_002944"
     CDS             15137..15442
                     /locus_tag="QBF46_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675101.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTP"
     gene            15549..16193
                     /locus_tag="QBF46_002945"
     CDS             15549..16193
                     /locus_tag="QBF46_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDQ6675102.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSGWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNIFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            16190..16936
                     /locus_tag="QBF46_002946"
     CDS             16190..16936
                     /locus_tag="QBF46_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDQ6675103.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVIAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAE
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPLGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            16936..19032
                     /locus_tag="QBF46_002947"
     CDS             16936..19032
                     /locus_tag="QBF46_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDQ6675104.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            19078..20217
                     /locus_tag="QBF46_002948"
     CDS             19078..20217
                     /locus_tag="QBF46_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDQ6675105.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITAHLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            20205..20651
                     /gene="etp"
                     /locus_tag="QBF46_002949"
                     /gene_synonym="yccY"
     CDS             20205..20651
                     /gene="etp"
                     /locus_tag="QBF46_002949"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDQ6675106.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            20671..>21893
                     /locus_tag="QBF46_002950"
     CDS             20671..>21893
                     /locus_tag="QBF46_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HDQ6675107.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVR"
BASE COUNT         5507 a         5520 c         5416 g         5450 t
ORIGIN      
        1 gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct gtagctgata
       61 gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc ctgccacagt
      121 tgcggtgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc actaaagagt
      181 gtgacagaaa tatgacattt ttccgggaac aggttcgcaa aatggcttgt tgaatgcctg
      241 tttttctatc tacatcttta aaaacaggca aactatgcct gaatagcccc tgcctcgcat
      301 acgccgctga tgattacacc agtgggctag acggaatatc agcggcgtta ccaatacaga
      361 ggcaatattt accaataaaa aaagtgtgag actaataaca atggtttata gcacatgcat
      421 ttgtcattaa attgcataac gatttaaata gtcaagttgc gcattcattt ccttattgtt
      481 taacttgtaa gcctgagcaa aaagttcagg agggaggtaa acgtcataat gattgccagt
      541 gtttattaaa taaccatact caagatcttc atctgttgaa aaggattcat tccctaagga
      601 gattgatgcc acattactat taacaataaa taatgagcaa atgctatctg aaagaacaga
      661 aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg gtaactaaag taagcaaatt
      721 cttgttatca ttaaatataa actcgggtaa atgcaggata catttttctt tattcaagca
      781 actatcaata aattgttttt aaaaaaaaag aaagctccat gacatgggac tacccacacc
      841 ctgaattttg ttgtcagatt gcagatcaac gccaaataat gtcttaacat cttccctgag
      901 atcatagaat gccgcatcaa caagagcctc gatcgtttgt ttatcaacaa ttttaataaa
      961 ctcttcgtat acttcctttt gtgtcttatg actgcccagc gcccatgaat aatcatgttg
     1021 ttttaaaacc agtatagctc ttaacagaca atatccatcg ccatctacag gagcatattt
     1081 taatcctaat gcctggttag tttcactctg aataaaagat ataccagaca aatcaatgtc
     1141 agaatccaaa tcagatattc ccggcgtatt tatccgctct ggctggatac tcatattttc
     1201 attatcatct aaacaatact cagaaaaaac atcatcacac gcagtaatgt tttcaatata
     1261 ttccataaag ccataccaat aataaccttt aaaatctgtg atagactctg accctctgcc
     1321 cggaaaggac actctaacaa tatcaatatc tcgaacatat tgtatagtat atgtaatacc
     1381 tcctgaaacc cctgaataaa tttgtttatc attagtacgc aatgttttca taaataatga
     1441 taaatcatta tcattctgta tttcctcaaa ttctttttct agtgaatcgc attccaggca
     1501 tggaatgtag tcttcagctt gttgacgtct gatttcttta tacaaccgca taaaacacac
     1561 cagtgtgttt tcaaaaagcg tgattgaata tgtttgattt ttttcgtact cattttttat
     1621 atcgttccag gcttgcttca aataattatt tgcagtgcaa ctaattccat gtatattacc
     1681 catatatagc gcctataaaa tttaataaat aatgacgccc cagttaaact taaagtgcct
     1741 ggttccacta tcaaaaatcg ctcacccttt ttcacctgtt taaaatatgt tcagcacccc
     1801 accttgaggc gacctcctct ccgcgatgat ttcaataaca tattctgtgt tggcatatga
     1861 aattttgagg attaccctac acttatagga gttaccttac aggggttcct tcaatttgtg
     1921 ttgatttacg cgagataacg ctatggaatt aaaggattat tacgccatca tgggcgtgaa
     1981 accgacggac gatctcaaga caatcaagac cgcctatcgt cgacttgccc gcaaatacca
     2041 tcctgatgtc agcaaagaac cggatgccga agcccgcttc aaagaggtcg ctgaagcctg
     2101 ggaagtgtta agtgatgaac aacgtcgcgc tgagtatgat cagatgtggc aacatcgcaa
     2161 cgatccgcaa tttaaccgtc agttccatca tggcgacggt cagagtttta atgccgaaga
     2221 ttttgacgat atcttctcgt caattttcgg tcagcatgcc cgccagagcc gtcaacgccc
     2281 cgccgcacgc ggccacgata ttgaaatcga agtggcggta ttcctcgaag aaacgctgac
     2341 tgagcataag cgtaccatca gctataacct gccggtttat aacgcctttg gcatgatcga
     2401 acaggaaatc ccgaaaacgc tgaatgtgaa gatcccggcg ggcgtcggca atggtcaacg
     2461 catccgtctg aaaggccagg ggacgccggg cgaaaacggt ggtccaaatg gcgatttgtg
     2521 gctggtgatt catattgcgc cacatccgct gttcgatatt gtcggccagg atctggaaat
     2581 tgtggtgccg gttagcccgt gggaagcggc gctgggtgct aaagtcaccg ttccaacact
     2641 gaaagaaagc attttgctga ctatcccgcc aggcagccag gccgggcaac gattgcgcgt
     2701 taaaggcaaa ggtctggtga gcaaaaaaca gaccggcgat ctgtatgcgg tactgaaaat
     2761 cgtgatgccg ccgaaaccgg atgaaaacac tgccgcgctg tggcagcaac tggcagacgc
     2821 ccagtcgtct tttgatccac gtaaagattg ggggaaagca tgatggctaa tgttacggtg
     2881 acttttacta ttaccgaatt ttgcctgcat accggcatct ctgaagagga gttgaatgaa
     2941 attgtcggtt tgggggtggt tgaaccgcgt gagatccagg aaacaacctg ggtatttgac
     3001 gaccatgccg ccattgtggt gcaacgcgcg gtacgcctgc gtcatgaact ggctctggac
     3061 tggccgggga tcgcggtggc gctgacgtta atggatgata ttgcacacct gaagcaggaa
     3121 aaccgcctgc tgcgccagcg actttcccgg tttgtagctc atccgtgatg tgtgtttgcc
     3181 ggatgtgatg acgcatccgg caactgcccc ataatgcctg atgcgacgct aaacgcatct
     3241 tttcaggcgc acaaacgacg ttatctgttt tggtggtcgc cctccactaa caccagcaat
     3301 aactggctta gtgccgcgta aaaaccaaag ctgtcatact gtcgacaacg cgcagcaaac
     3361 tctggtaacc attgccgcag cgccgtcagt gttttttgcc gtaaactgtc gattcttcgc
     3421 gcaggaaccg tcccctctcc cagcgaaaaa tgcagatgac tcaacaactc gagatagatc
     3481 gccagatgat ctgccggttc gttgaaattg ccgctggttt ccatccctgc ctcaactaac
     3541 aagcgtttaa tctcttgctc gtcctgtttg taggccgatg catacggcag cgccgcttgt
     3601 ttgtcggtca tcagaaacag gccgcaaaag tccgcggcca gttccagacg ggcatcgtca
     3661 cgtactgtca gcgtggcaat acggttttcc agctcgttca ccgccgcagt gagcggcggt
     3721 tcgcttttca gcaacgaaaa ccattcagcc atctgcgcac tggcgatttg cgtcagttgt
     3781 tcatcgtcca gttcacggga gaacaactgc gccagccagg cgtaaacaca ggcaatctgt
     3841 tgtgctgtca gcgtggtcat gatttcacct gcgacgcggg aacatattcg cactgcgcca
     3901 ccatctccac ggggccgtta aacgccgtca cctgctccac tgttccgttg tacttctcaa
     3961 tttccaccag cgtagtgtgc gcactggtcg cctgcgccag ctgcgaagta ccgatgtcga
     4021 tggtcagcac gttggggtta ccgtatttgc acagcgcacc aggctcgccg cctttatctg
     4081 gatcgtacca tgccccttcg tgaattcgcg ccacgcctgg tgcatagcgg tcagaaacta
     4141 ctgctcctgc caacacctga ccgcgagcgt taaatacgcg taccacatca ccgttacgaa
     4201 taccgcgcgc gctggcatcc tgcgggttaa tgaacactgg ctctttaccc gctaccgtat
     4261 attgctgacg cagcgtttcc gactcacata actgcgagtg aagtcggaaa tccggatgca
     4321 cagattgcag atgcaacgga tacgtctgcg agccaggccc accgtgggag cgttcgattt
     4381 tctcaaacca catcggatgc ccctgacaat cgtcgtagtt catatcagcg atggttttcg
     4441 agtagatctc aatcaggcca ctcggcgtgc ccagcggttc gagatccgga tcttcgcgga
     4501 atgcctggtg gcgaacaaac atctgcggat ggtcaaactc gacgtactct ttgttattcc
     4561 agaagtcatc aaacgctggc agatgaacgc cgcgtccttt gccttgttgt acaccttcct
     4621 gccagatgcg tttcagccag cccatttcgt ccagcccttc ggtaaaggct tcttcgcgat
     4681 taaagcggcg gcacagctcg cggaaaatat caaagtcgtt gcgcgcctcg aactgcggcg
     4741 gcaccacctg tttcatggcg ataatgccac ggttggagtg gttgccgtac tggtcgagat
     4801 cgttacgctc aaactgcgtg gtcgcaggca gcacgatatc ggcaaagcgg caggttgagg
     4861 tccactggtt gtctatggcg ataaccgttt ccagcttgcg ccagccttca atgatgcggt
     4921 tgatctgctg atggcgatgg aacgggttag ttccggcaaa aatacacatt ttcagcggcg
     4981 gcagttttac cgatttaccg ttccagttga tcactttccc cggttcgagg atcgcatcga
     5041 taaaacgggc aatcggaatg gtgctgctgt agcctttgta atcactgttg tcgtgaacag
     5101 gcggaatcga cgtagagccg gagaaaccac tcagaataac gcctttacgc cccggcgtgc
     5161 ctgcgccgtt gtagtgccag ccaaaaccaa aaccaccacc tggcaggcca atttgcccca
     5221 gcatcgccgc cagaaccaca atcatccacg cccactgttc accgtgctgc atacgctgta
     5281 cgcaccagcc agcaataatc tgcgttctgt tcgccgccat ctggcgcgcc aacccacgaa
     5341 tggtttcggc atcaatacca gtcagttttt cagcccatgc ggcatctttc ggctgaccgt
     5401 ctttctcacc cagcaggtac ggcaggaact gctcaaaacc tacacagtag ttagcgagga
     5461 agtttttgtc gtacaggttt tcactgtaca gcgtatacgc cagcgccagt tgcagcggca
     5521 cgtcagtttg cgggttaacc gcaatgtgct tcacatgctc gcgccccaga tactcatggg
     5581 tggatgtgac aaccggatcg atgctgatga cctcaatttc accggcggcg actttcgctt
     5641 ttagctgctc gtaatattca taaacatcgt gatccgggca ccaccagttc gcttgctggt
     5701 ttttcagcaa atcggagccc cacagcacaa tggttttgct gttctgcaat accagcggcc
     5761 aggaggtttg ctgttcatac acttccattg aaccgactac gcgcggcagg atcacctgcg
     5821 cagcaccggt agagtaatct ccgcccgtac caacgctatt accatgcaag gcaatagctt
     5881 tcgccagcat ccccgaagcg ttatggaaca tccccgtcga ttgccaacca ctggcggtca
     5941 gcaaggcact cggcccgtga gttttctgta cgcgttccag ttcttcatag aacatgtcga
     6001 gggcttcatc ccagctcacg cgcacaaaac ggttatcacc gcgctgggag gtgtcgctca
     6061 gatggcgctt acgcagccag tccacgcgta ccatcggata acgaatacgt gccgcgttgt
     6121 gtacgtgatc cggcaatccg gcgatcattt tcgacggata tttatccagt tcgaacggtt
     6181 ttgccgccac aaagcgacca tccttcaccg tcgcgcggat agccccccag tgcgacccgg
     6241 taagaatgcc ctctttcgag atgacagcct cagtcgccgc ttgcgccgca gttgcacggc
     6301 gcggcgttaa caatgacggc cccagcatcc cggcgacggt taagccgccg agttgtgcca
     6361 gaaaacgccg acgtgatgcc tgaaagagat cgttattgtt cattattttt cttccttctt
     6421 atcgccgtga gccttacctg cggtgtcaga cgcattcatt tgcagatatt tcaacaaagt
     6481 gcgttcttca cgtttatcga gactggtaaa gccaatcatg ccgttgagcg tgccgatcca
     6541 accgttagcg tcaaagtggg cgatttccgg tgcgccgtgg cactggttac aggtgccgtt
     6601 gtacaacgaa tccgcatagg cccagatcgg tttgatatcg ttcaccatgt cgcctttctt
     6661 catccatgcg gtggcctgca acttgctcca ctcggtattg gtgtcggcaa cggtggtttt
     6721 ctccagcgtt ttcacctgct gctgcacatc accacgaatc gaggcaacaa agatgcgttt
     6781 acctgggaat tgggtgagta cacgctgacg tccggcgctt tccgtccagc cggtaatttc
     6841 aatttgcagc cagtcgccgt cacgtttaag gactttcact tccgaagcag gcagcagaga
     6901 accagaggct tctttatcgc ctttcgccgc ataaattggc ttaatatcaa tagagtacag
     6961 cgtgtcacca ctgtcattag cactggcgcg cagctcatcg aactgcttac ggaagccgct
     7021 actcatatcc ggtaactggt gggcaatacc tttatgacag tcgatgcagg attgattatc
     7081 tttcgctgcc accttcatct gacgtgccgc ttcaggatgc tgcttcgcat gatccatcgc
     7141 atcgtagtta tggcaggagc ggcaggttgc cgagttgttt tctttcattc gcgcccattc
     7201 acgctcggca agttccgcgc gtttggcttc gaatttttca ggtgtatcaa tggagtgagc
     7261 aataaaggtc tggtagatat cattgctcgc ttccagtttg cgcttcacca tgcctggaat
     7321 atccggcggg atatgacagt catggcattc agctcgcacg ccggaggcgt tctggaaatg
     7381 caccgactgt ttatattctt catacaccgg ttgcatactg tggcaactga cacaaaattc
     7441 ggttgtgctg gtgactttga tcccaacgtg tggcaataca atcagcgcaa tgccaatcac
     7501 aatcccaatt gcgaccagcg ccagtaccga ccaacgagca ctgggtcggc gtagcgcgtt
     7561 ccagagtttc cgcataatag cccctgtaaa attatggttt agtgaagcga tcttaatgag
     7621 caaatatgaa cagcggcact ggtcaggatg aacggcttac ggcagaatat gaacagatat
     7681 gaacagaatg agtaaaaccc tctgatgcca catcacattg ttattgttga agatgagccg
     7741 gttacccagg cgcgattaca atcttacttc actcaggagg ggtataccgt ttccgttaca
     7801 gcgagcggtg ccgggctgcg ggaaattatg cagaatcagc cggtagattt aattctgctg
     7861 gatatcaact tacccgatga aaatggcctg atgttaaccc gcgccctgcg agaacgctca
     7921 acggtgggga ttattctggt taccggacgc agcgatcgga ttgaccgtat tgttgggctg
     7981 gaaatgggcg cagacgatta cgtcaccaaa ccgctggaac tgcgcgaact ggtagtacgg
     8041 gtgaaaaatc tgctctggcg aatcgacctc gcgcgacaag ctcaaccgca cactcaggac
     8101 aactgctatc gctttgccgg ttattgcctg aatgtgtcgc gccatacgct ggagcgggat
     8161 ggcgagccga ttaaactgac ccgcgcagag tatgaaatgt tggtggcatt tgtgacgaat
     8221 ccgggcgaaa ttctcagccg tgaacgtctg ctacgtatgc tttctgcgcg tcgggtggaa
     8281 aaccctgacc tgcgcaccgt cgatgtgtta attcgtcgtt tacgtcataa actcagcgcg
     8341 gatttactgg tgacgcaaca tggtgaaggt tatttcttag ccgctgatgt gtgctgataa
     8401 aaatagaccg gacgaaatcc ccctggtgac agcgagcggc gaatatgttc acggtcggca
     8461 tttttcggcg tcagaactaa aatcggtggg ctgacattat cagacaccga ttgcccctgt
     8521 aattgcctga tggcctgctc aactgccagt tccccctgcc agaccatttg atcgctggca
     8581 gccataatca ctcttccccg cttcagcccg cgatacacct gatgtgaaag ataaaacgac
     8641 accacggtaa gcggcgtttt caggttacgc ccttcaccca ttgccgcctc tgccgcaatg
     8701 gccgttccgg caacgacgtc gatttctggg tggcgttcca gcatctcctg caacaggtta
     8761 cgctggattt caatatcgtt atcaccaagc gcaatatcaa caatacgcac cgggcttccg
     8821 gcaatggctg cgcgaaaacc ctcgaccatc tctttactgc ccccggcatt atcgggtccg
     8881 ggcatcaaca gcacgttcag tggtttaccg tgcgcccatt gcaccaaata tcgcccaggt
     8941 tgatagccca tctgaaacca aggtacacca acgcggcttt tcacctgggg agcatcaata
     9001 gcatttacca gttcgatcac cggcagactt gctacctgct tttgcagatc gggaaatgag
     9061 gtcgtgctac taccgagtaa aatggcctct gcgccccact gtttacactg gtcgatttgt
     9121 gcttgctggg tagccaactg gctgtagccg cctgcctcca gcacttttaa atccacacca
     9181 taacggcgag ctgcctcctg cataccatag ttcaacgata accagtatga atctttcagg
     9241 ctgggataaa gcgcgcacag tttccatgcg cgtttggctt taagcggcat agaggcttgc
     9301 accgtgaaat gctgcgcatc atgccagcgc aacaggttat cagccgaaaa tgccggcaac
     9361 atgaaaaggg aaagaagtaa aaatagcagt acgcgcatga tagcctcatc aataataagg
     9421 ctttatgcta gatgcattcc gctttgcgac tcaacctttt tcaccttaag tgcaccgacc
     9481 gtgaatttaa ccctgacccg aagactctgg atgggctttg ccctgatggc gctgttaacc
     9541 ctgaccagta ccctggtggg atggtacaac ctgcgcttta tcagccaggt ggaaaaagac
     9601 aacactcagg cattgattcc taccatgaat atggcgcgcc agttgagcga agccagcgcc
     9661 tgggaacttt tcgccgcgca gaacctgacc agtgccgata acgaaaagat gtggcaggcg
     9721 caggggcgaa tgctcaccgc acaaagcctg aagattaatg cgttgctgca agcgttacgg
     9781 gaacaaggtt ttgataccac cgctattgaa caacaggagc aggagatctc ccgttcatta
     9841 cgtcagcaag gggaactggt ggggcggcgt ctgcaactac gccagcaaca acggcaactc
     9901 agtcagcaga tagtcgctgc cgccgatgag atcgcacgcc tggcgcaagg tcaggcgaat
     9961 aatgcgacaa cttccgctgg agcgacccag gccgggattt acgatttgat cgaacaagat
    10021 cagcgtcagg ctgctgaaag tgcactcgat cggctgattg atatcgatct tgagtatgtt
    10081 aaccagatga atgaactgcg ccttagcgct ctgcgggtgc agcaaatggt gatgaatctg
    10141 gggctggagc agatccagaa aaatgcacca acgctggaaa agcagctcaa taatgcggtg
    10201 aaaattctgc aacgtcggca aatacgcatt gaagatccag gtgttcgtgc gcaggtcgca
    10261 acaacgttaa ctaccgttag ccaatatagc gatttgctgg cgctgtatca gcaggacagt
    10321 gaaatcagca atcacctaca aactctcgcc caaaataaca tcgcccagtt cgcgcagttt
    10381 agtagcgaag tcagtcagct ggtcgatacc attgagctgc gtaatcagca cggactggcg
    10441 catctggaaa aagcgagtgc acgcgggcaa tacagcctgt tattgctggg aatagtttct
    10501 ctttgcgcac tgattctgat cctctggcgc gtggtttatc gctcagtcac gcgtccactt
    10561 gccgaacaaa cgcaggcgct gcaacggctg ctggacggtg atattgactc ccctttcccg
    10621 gaaaccgctg gcgtacggga gctggatacc atagggcggc tgatggatgc gtttcgcagc
    10681 agtgttcatg cactgaatcg ccaccgtgaa cagctggcgg cgcaggtcaa agcgcgtaca
    10741 gctgaattgc aggaactagt gatagaacac cgacaggcac gggcggaagc agaaaaagcc
    10801 agccaggcaa aatcggcgtt tctggcggcg atgagccatg agatccgcac accgctgtac
    10861 ggtattctcg gcaccgcaca gctactggca gataaccccg cacttaacgc ccagcgtgat
    10921 gatttgcggg caattactga ctctggtgaa tcgttgctga ccatcctcaa cgatattctc
    10981 gattattccg ctatcgaagc aggtggcaag aatgtttcgg tcagcgatga gccctttgaa
    11041 ccgcgcccgc tgctggaaag taccctgcaa ttaatgagcg gacgggtgaa aggtcgcccg
    11101 attcgcctgg caacggcaat tgccgatgat atgccgtgcg cgttaatggg cgatccacga
    11161 cgtattcgtc aggttataac caacctgttg agcaacgccc tgcgttttac tgacgaaggg
    11221 tacattatcc tgcgtagtcg cactgatggc gagcaatggc tggtcgaagt ggaagacagc
    11281 ggctgcggta ttgatcccgc gaaactggca gaaatcttcc agccatttgt gcaggtaagc
    11341 ggcaaacgcg gcggcaccgg gctggggctg actatcagta gccgtctggc ccaggcgatg
    11401 ggcggcgaac tgagcgccac cagcacgccg gaggttggaa gctgtttttg tttacgcttg
    11461 ccgttacgtg ttgccacggc acccgtgcca aaaacagtca atcaggcggt gcgtcttgac
    11521 ggtttacgtt tgctgttaat tgaagataac ccgctaaccc agcgaattac cgttgagatg
    11581 ctgaacacca gtggtgcgca ggttgttgct gttggcaatg ccgcgcaggc tttagagaca
    11641 ctgcaaaata gcgaaccgtt tgctgccgca ctggtggatt ttgatctgcc ggatgtcgac
    11701 ggcattacgc ttgcccgaca actggcacag caatatccgt cgctggtttt gattggcttt
    11761 agcgcccatg tcattgacga aacgctgcgc cagcgtacca gttcattatt ccgcgggatt
    11821 atccctaaac cggtgccgcg tgaagtgctc ggtcagttac tggcgcacta tctccaactg
    11881 caagtcaata atgatcaatc gctggatgta tcgcaactca atgaagatgc tcagttaatg
    11941 gggacggaga agatccacga atggctggta ttatttacac aacatgccct gccgcttctc
    12001 gatgaaatcg acattgcccg cgccagtcag gatagcgaga aaataaagcg tgccgcgcat
    12061 cagctaaaaa gcagttgctc gagtctggga atgcgtatcg ccagccagct atgcgcacaa
    12121 ctggagcagc agccattatc tgcccccctt ccacacgaag aaattacacg cagtgttgcc
    12181 gctctggaag catggttgca taagaaagac ctgaacgcga tttgaatcaa tatcagccag
    12241 ttaagaacaa ctagaatagt ggcatttggt atacaactta acggactggc aaaatcatgg
    12301 cagagaataa aagaacccgc tggcagcggc ggccaggcac gacgggcggc aaattaccgt
    12361 ggaatgactg gcgtaatgcc acgacctggc gtaaagcgac gcaattatta ctgttggcaa
    12421 tgaatattta tattgccatc acgttctggt attgggtgcg ctattacgaa acggcaagta
    12481 gcacgacatt tgtcgccaga ccgggaggta tcgaaggctg gctaccgatt gccgggctga
    12541 tgaatctgaa atatagcctt gtaacaggtc agttaccgtc cgtccacgcc gccgcgatgc
    12601 tgttattggt cgcttttatc gtcatcagtc tattactcaa aaaggccttt tgctcatggt
    12661 tatgcccggt tggtacgctt tctgaattaa tcggcgatct cggtaacaaa ctgtttggtc
    12721 ggcaatgtgt ccttccccgc tggctggata ttcctctgcg cggcgtgaag tatttgctgt
    12781 tgagtttttt tctctatatc gcgttattga tgcccgctca ggcgattcac tattttatgt
    12841 tgtcgcccta cagcgtggtg atggacgtta aaatgctcga tttctttcgt catttgggga
    12901 ccgcgacatt aatcagcatg accgttttgc tgattgccag cctgtttatt cgccatgcct
    12961 ggtgtcgtta tctttgccca tatggcgcgc tgatgggcgt ggtttcgcta ttgtcgccgt
    13021 ttaagattcg tcgcaatgcc gaaagttgta tcgactgtgg caaatgcgca aaaaattgcc
    13081 catcacggat cccggtcgat aaattaattc aggtacgaac agtggaatgt accggctgta
    13141 tgacttgcgt agagtcatgt ccggtagcct caacattgac cttttcactg caaaaacctg
    13201 cggcaaataa aaaagccttt gcgttgtctg gctggttaat gacgctactg gttctgggga
    13261 ttatgtttgc ggtgattggt tacgcaatgt atgcgggagt atggcaaagc ccggtaccgg
    13321 atgaattgta ccgacgctta attccacaag cgccaatgat tggtcactaa attagcaaca
    13381 agcggaacat cacctcgcac gggtgatgtt cacaaaatca cattattttg attttgacat
    13441 cataactttc aagcccggtc attttttctc gtgcggtgaa gcgaatttca gagacttctt
    13501 tccctgtatt cttgttcagc tcggcgattt tttgcgcgat aaagtcggcg atttccgttt
    13561 cggcttgttt ttttaactct tcaatattca ctgttaacct cttctggtct tgaaagacag
    13621 caacacgcca gtaaggtgtt ggttcccggt accctggtca ggtaagaaat gttataagcc
    13681 atctccccga aagattcaat aatcaacagg ttatcgctta ctatacatac agcgttgaag
    13741 aaaaataagg cagatataaa aaaggctcca cgagggagcc tgatattatt ttgtgaaatc
    13801 cagcggcgca tggtccgtgc gtatttgcag cgcgacgtta tcaaaggtga tcatcgtcga
    13861 tttacaaaaa atacacttag cgcccaaagg gtttcgctcc gtcacatcga atgccgatgt
    13921 acggtactgt gaaccatgac aacatggaca gcggaaatgg agatgttgag taataacggc
    13981 aaccttagag cgtaacaacg ttcgctgccg cggggccacg ttgcccctgc tcaatagaaa
    14041 attcaacttt ctgattttcg ttcagcgtgc ggaattcatt gctctggatg gcagtgaaat
    14101 ggacgaaaac gtctttgctg ccatcatcag gagtgataaa gccaaaacct ttatctgcgt
    14161 taaaccattt tactaaacca gtcattttat tagacatatt ttacttcctt cattttgggc
    14221 ctttcggcaa acagggcttt aatacagaaa ctacttagtg ttcaggagag agactcaaaa
    14281 gaaggggata atgcctgata atgagaactg ctttagtaaa ctactttgta tgctgtctgt
    14341 ctttcaaacc gacgcagcta ttaacgcatg ataattgaga ttaagcaaat tttattttag
    14401 ccgtccggcg gtcttaaata tcgctggctt tattttatta accccgtata gtgcagcgca
    14461 gtattttcag taaggaattt gtttgtcccg taaaatgaca ggaattgtca aaacctttga
    14521 tcgcaaaagc ggcaaaggat tcattatccc ctccgacggt cgtaaagaag tccaggtcca
    14581 tatttccgca ttcactcccc gcgacgcaga agtgcttata ccaggattac gcgtggaatt
    14641 ttgccgcgta aatggcctgc gaggaccaac agcggcaaat gtttatctct cttaatatga
    14701 cgctgcttgt ttaaagctga ctggttttat tggaatagtt caatagtcaa caccataaat
    14761 tataatatga gttacttttt caccaggcaa caaatccatt taagcgaata tctctgcacc
    14821 taatagttat aaatgccact taacagccag ttatagtacc acttgaaact atattaccaa
    14881 caatagttaa tagctttctt aacaattttc ttatctttaa tttgtatttt tctaagattt
    14941 atcttatgcg cacaagtcgt atttccagag ggaaatcgtt aacaatctta tatttccctc
    15001 tgcgcgcctt tctcacccgc tgtatatagt gcaagtggta tgaggaaagg gagatttcac
    15061 aaggatgtta tactcaaagt agacaatcct gatctcaagc gtacgtattg tccatgcgat
    15121 gaataaagga aaagttatga aacacaagct ttctgcaatc cttatggcct tcatgttgac
    15181 gacgccagct gcgtttgctg cccctgaagc agcaaacggg accgaggcaa cgacgggtac
    15241 tactggcacc acaacaacca ctacaggtgc aaccacgact gctgctacca ctggtggcgt
    15301 ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg gcaacagctg ttggtgtggc
    15361 gacgttggca gtagttgcag caaatgacag tggcgatggc ggttctcata acacctcaac
    15421 caccacgtcc actacgccct aatactgctt taacgcgtcg aaattaaagc taaataatac
    15481 ccggttgcga tattcgtacc gggctttaga gatttgcatt tttttaatta acctttcaag
    15541 gattaaaggt gcgccctctt attttatcga ttttcgcact atttcttgcg ggatgtacgc
    15601 acagccagca aagtatggtc gatacatttc gcgccagcct tttcgataat caggatatca
    15661 ccgtagcgga tcagcagatc caggcgttgc cttattccac gatgtattta cgccttaatg
    15721 aagggcaacg aatctttgtg gtactgggat atatagaaca agaacaaagc aaatggttat
    15781 cccaggataa cgccatgctg gttacccaca atggacgtct tttaaagacc gtcaaactta
    15841 ataataatct gctggaagtg acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa
    15901 taaaagacgg cagcggctgg acgcgcgata ttctctggag tgaagacaac cattttcgct
    15961 ctgcgaccct gagttccact ttttcctttg ctggattaga gacgctgcat attgcgggtc
    16021 gcgatgtgct gtgtaatgtc tggcaggaag aggtgacttc cacgctgcca gaaaaacagt
    16081 ggcaaaacat attctgggtc gattcggcta ctggccaggt tcgtcaaagt cgacaaatgt
    16141 taggcgcagg ggttattccc gtagaaatga cgtttcttaa acccgcacca tgaataaatt
    16201 acagtcgtat ttcattgcca gcgtacttta cgtaatgaca ccccatgcct ttgcgcaagg
    16261 aacggtgact atttatctgc ctggcgaaca acagacactt tccgttggcc ccgtggaaaa
    16321 tgttgtccag ttggtgacac aaccgcaact gcgcgatcgc ctctggtggc ctggtgcctt
    16381 gctgaccgac tctgcggcga aagctaaagc gcttaaagac taccaacatg tcatcgcaca
    16441 acttgcttcc tgggaagcag aagctgacga cgatgtggcg gcaacgatta aatccgtgcg
    16501 ccagcagtta ctcaatctca atatcactgg tcgcttaccg gtcaaactgg accctgattt
    16561 tgtacgagtg gatgagaaca gcaatccacc gctggtcggg gactatacgc tctataccgt
    16621 acagcgcccg gtgactatta ctctgctggg tgcggtttct ggggctgagc aattgccgtg
    16681 gcaggcaggc cgtagcgtta ccgattatct gcaagatcac ccccgtcttg caggcgcgga
    16741 caagaataac gttatcgtga ttacgcctga gggtgagact gtcgtcgccc ctgttgccct
    16801 gtggaataaa agacatgtgg aaccaccgct tggcagccag ctttggttgg gcttttctgc
    16861 gcatgtactg cccgaaaaat atgctgattt aaatgaccag attgtgtctg ttttaacgca
    16921 acgtgttcct gactgatgaa gaagaattct tatcttttaa gctgcctggc cattgctgtc
    16981 tccagtgcct gtcatgctga agtattaacc tacccggatc cgctgggttc gtcgcaatca
    17041 gactttggcg gcacaggatt gttgcagatg ccaaatgcgc gcatcgcacc ggaaggtgaa
    17101 ttcagcgtca actaccggga taacgatcaa taccgtttct actccacctc cgtggcgctg
    17161 ttcccatggc tggaaggcac cattcgttat acggatgtgc gcacacgtaa atatagtcag
    17221 tgggaagatt tcagcggcga tcagtcatac aaagacaaat cattcgattt taaacttcgc
    17281 ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg gtaaacgcga tattgctggt
    17341 acgggtctgt ttgacggtga gtatctggtg gccagcaagc aagcggggcc atttgatttc
    17401 accctcggga tggcatgggg ctacgccggt aatgcgggca atattaccaa cccgttttgc
    17461 cgggtgagcg ataaatattg tcatcgcgca gagtctcacg atgcgggcga tatcagcttt
    17521 agcgatatct ttcgtggccc ggcctccatc tttggcggca ttgagtatca aacgccgtgg
    17581 aatcccctgc gtctgaaact cgaatacgac ggcaacaatt accagaatga tttcgctggc
    17641 aaactgcctc aggcaagcca tttcaacgtc ggtgcagttt atcgcgctgc cagctgggca
    17701 gacctcaacc tgagttatga acgcggtaac acgttgatgt ttggtttcac tttacggacc
    17761 aatttcaacg atctgcgccc tgccctgcgc gatacgccaa aaccggcata tcaacctgcg
    17821 cctgaatctg aaggattgca gtacaccacg gtagcaaacc aacttaccgc cctgaagtat
    17881 aacgcgggct ttgacgcgcc agaaattcag ctacgcgata agacgctgta tatgtctggt
    17941 cagcaataca aataccgtga ctctcgcgaa gcggtcgatc gtgccaaccg gattctggtg
    18001 aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg
    18061 atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac agctggccgg tacagcgcct
    18121 ggtcaatcag agccactgca acaacaacgt gttgaagctg aagatctttc tgcctttggc
    18181 cggggctacc gtattcgtga agatcgcttt agctactctt tcaacccaac actttcacag
    18241 tcgctgggcg gcccggaaga tttctatatg ttccagttag ggctgatgtc cagcgcccgc
    18301 tactggttta ccgaccacct gctgcttgat ggcggtattt tcaccaatat ttacaacaac
    18361 tacgacaagt ttaagtcttc gctgttaccc gcggactcta ccctgccccg cgtgcgcacg
    18421 catatccgtg attacgttcg caatgacgtt tatctcaaca acttgcaggc gaactacttt
    18481 gccgacttag gcaatggttt ctatggccag gtgtatggcg gttatctgga aacgatgtat
    18541 gccggtgtcg gttccgagct gctttatcgc ccgctagatg ccagctgggc gctgggtgtg
    18601 gacgttaact acgtgaagca acgtgactgg gacaacatga tgcgcttcac cgattattcc
    18661 acgcccacag gtttcgtgac ggcttactgg aacccgccga cgctcaatgg cgtactgatg
    18721 aaacttagcg ttgggcaata tctggcaaaa gataaaggcg caacgatcga cgtcgccaaa
    18781 cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt atctaaagat
    18841 gactacggcg aaggcggctt tagtaaaggt ttttatatct cgattccgtt cgacttgatg
    18901 accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt
    18961 ggacaaatgc tgtcacgcaa ataccagctc tatccaatga cggcagagcg agaagtaccg
    19021 gttggacaat aacctgacgt aattcataac attacattga ctgataaata taaaccaatg
    19081 aaaaagaaca tttttaagtt ttcggtactg acattggctg ttctgtcttt gacagcatgt
    19141 acccttgttc ctggtcagaa tttatcgacc agtaacaagg atgtcattga actacctgac
    19201 aatcagtatg acctggacaa aatggtcaat atctatcccg tcacgccagg attgattgat
    19261 cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg agctggagca gcaaatcgcg
    19321 aactatgaat atcgtatcgg cattggtgat gtgttgatgg ttacggtctg ggatcacccg
    19381 gaactaacca cccccgcagg gcaataccgt agcgccagtg atactggcaa ctgggttaac
    19441 gccgatggtg cgattttcta cccctatatt ggtcgcctga aagtggcggg gaaaacactt
    19501 acccaggtac gcaacgagat tacagcgcat ctggattcag tcattgaaag cccgcaagtc
    19561 gatgtcagcg tcgcggcgtt ccgctcgcaa aaagcatacg tcaccggcga agtcagcaaa
    19621 tcgggtcagc agccgattac caatattcct ctgaccatca tggatgcgat taacgccgcg
    19681 ggcggtctga ccgcagatgc agactggcgc aatgtggtgc tcacccagaa tggcgtaaag
    19741 accaaagtga acctctatgc gctgatgcag cgtggcgact tgcgccagaa caaactgctg
    19801 cacccgggcg atattttatt tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt
    19861 gaagtgggta aacagagcac gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg
    19921 ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg cgacggggat ttttgttatt
    19981 cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata tttatcagct gaatgcgaag
    20041 gacgcatcgg caatgatact cggaacggaa ttccagcttg aaccttacga cattgtttat
    20101 gtcactaccg cgccgctggc tcgttggaac cgtgtgattt ctctgctggt accgacgatt
    20161 tccggggtgc atgacctgac agagacttca cggtggattc aaacatggcc caactaaaat
    20221 ttaactcaat cctggtggtt tgtaccggca acatttgccg ttcgcccatt ggcgagcgct
    20281 tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat ggtctggtaa
    20341 aacaccctgc agatgcgaca gcggcagatg ttgccgctaa tcatggcgta tctcttgaag
    20401 gacatgccgg acgtaagctc actgctgaga tggcacgaaa ttacgatctg attctggcaa
    20461 tggagtcgga acatattgct caggttaccg caatcgctcc cgaggtccgc gggaagacaa
    20521 tgctgtttgg gcaatggctg gaacagaaag agatcccgga tccctatcgt aaaagtcagg
    20581 acgcatttga acatgtctac ggtatgttgg agcgcgccag tcaggaatgg gcgaagcgcc
    20641 tcagccggta atttgagttt ataaaatacg atgacaacta aaaatatgaa tacgccacca
    20701 ggcagcactc aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt atgggatcac
    20761 cgtaagttta ttatcagtgt gactgcgtta ttcacgctga tcgctgtcgc ttactcgctg
    20821 ttaagcacac caatttatca ggcagatact ctggtccagg ttgagcaaaa acagggcaac
    20881 gccattctca gcggcctgag cgatatgatc cctaactcat cgcccgagtc tgcaccggag
    20941 atccaactgc tgcaatcgcg catgattctc ggtaaaacca ttgctgaact gaatctgcgc
    21001 gacatggttg agcagaagta ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa
    21061 gagaaaccag gtgagctggc gatcagctgg atgcatattc cacaactgaa tggtcaggat
    21121 cagcaactga cactcacggt tggggaaaac ggccactata cgctggaagg tgaagagttc
    21181 accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg
    21241 gacattaagg ccaaaccagg aacacagttt gtcctgagcc agcgtaccga actggaagcg
    21301 attaacgcgt tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag cgggatgctg
    21361 gaacttacca tgaccggtga tgatccccag ttgattactc gtattctgaa cagcatcgct
    21421 aacaactatt tgcaacagaa tatcgctcgc caggcggcgc aggattcaca aagccttgaa
    21481 ttcttacagc gccagttgcc tgaagtgcgc agcgagctgg accaggcgga agaaaaactc
    21541 aacgtttatc gccagcagcg cgattcggtt gaccttaacc tggaagccaa agccgttctt
    21601 gagcagattg tgaacgttga taatcaactc aatgagctga ctttccgcga ggcagagatc
    21661 tcccagctgt ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa acgccagacg
    21721 ctggagcaag aacgcaaacg cctgaataag cgggtatcgg ctatgccttc cacccaacag
    21781 gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct gcaattactt
    21841 aaccgccagc aggagttgag tatttcgaaa tccagtgcca ttggtaacgt gcg
//
LOCUS       DAOFQD010000075        21669 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.075, whole genome shotgun sequence.
ACCESSION   DAOFQD010000075 DAOFQD010000000
VERSION     DAOFQD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.075"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            86..463
                     /locus_tag="QBF46_002951"
     CDS             86..463
                     /locus_tag="QBF46_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDQ6675108.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            616..1167
                     /gene="apt"
                     /locus_tag="QBF46_002952"
     CDS             616..1167
                     /gene="apt"
                     /locus_tag="QBF46_002952"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDQ6675109.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            1296..3227
                     /gene="dnaX"
                     /locus_tag="QBF46_002953"
     CDS             1296..3227
                     /gene="dnaX"
                     /locus_tag="QBF46_002953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDQ6675110.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    2560..2624
                     /gene="dnaX"
                     /locus_tag="QBF46_002953"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            3280..3609
                     /locus_tag="QBF46_002954"
     CDS             3280..3609
                     /locus_tag="QBF46_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDQ6675111.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            3609..4214
                     /gene="recR"
                     /locus_tag="QBF46_002955"
     CDS             3609..4214
                     /gene="recR"
                     /locus_tag="QBF46_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDQ6675112.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            4324..6198
                     /gene="htpG"
                     /locus_tag="QBF46_002956"
     CDS             4324..6198
                     /gene="htpG"
                     /locus_tag="QBF46_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDQ6675113.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            6378..7022
                     /gene="adk"
                     /locus_tag="QBF46_002957"
     CDS             6378..7022
                     /gene="adk"
                     /locus_tag="QBF46_002957"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDQ6675114.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7258..8220
                     /gene="hemH"
                     /locus_tag="QBF46_002958"
     CDS             7258..8220
                     /gene="hemH"
                     /locus_tag="QBF46_002958"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDQ6675115.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8217..9176)
                     /gene="aes"
                     /locus_tag="QBF46_002959"
     CDS             complement(8217..9176)
                     /gene="aes"
                     /locus_tag="QBF46_002959"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDQ6675116.1"
                     /translation="MKPENKLPVLELISAEMKAVVNTIQPDLPLWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATSAYMVPTPYGQVETRLYSPKSHSPATLFYLHGGGFILGNLDS
                     HDRIMRLLANYTQCTVIGIDYTLSPEARFPQAIEEIVAACCHFHQQAGDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAAVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            9328..10632
                     /gene="gsk"
                     /locus_tag="QBF46_002960"
     CDS             9328..10632
                     /gene="gsk"
                     /locus_tag="QBF46_002960"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDQ6675117.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQIQPENETSASWVVGIDQTLV
                     DIEAKVEDDFIKRYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEEVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQEFLKEHVSILAMNEEEAESLTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDDAKRKTQHPLLPGAIPEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKLTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(10715..12391)
                     /locus_tag="QBF46_002961"
     CDS             complement(10715..12391)
                     /locus_tag="QBF46_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDQ6675118.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(12629..13849)
                     /gene="fsr"
                     /locus_tag="QBF46_002962"
     CDS             complement(12629..13849)
                     /gene="fsr"
                     /locus_tag="QBF46_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDQ6675119.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14067..15719
                     /gene="ushA"
                     /locus_tag="QBF46_002963"
     CDS             14067..15719
                     /gene="ushA"
                     /locus_tag="QBF46_002963"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDQ6675120.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(15756..16235)
                     /gene="ybaK"
                     /locus_tag="QBF46_002964"
     CDS             complement(15756..16235)
                     /gene="ybaK"
                     /locus_tag="QBF46_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDQ6675121.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(16439..17233)
                     /locus_tag="QBF46_002965"
     CDS             complement(16439..17233)
                     /locus_tag="QBF46_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDQ6675122.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            17371..17712
                     /locus_tag="QBF46_002966"
     CDS             17371..17712
                     /locus_tag="QBF46_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDQ6675123.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(17927..20431)
                     /gene="copA"
                     /locus_tag="QBF46_002967"
     CDS             complement(17927..20431)
                     /gene="copA"
                     /locus_tag="QBF46_002967"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDQ6675124.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTPGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            20693..21625
                     /gene="glsA"
                     /locus_tag="QBF46_002968"
     CDS             20693..21625
                     /gene="glsA"
                     /locus_tag="QBF46_002968"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDQ6675125.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAEGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         5392 a         5729 c         5728 g         4820 t
ORIGIN      
        1 gcagggcggt tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc
       61 actctgtaag ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct
      121 ggcggtagtg ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat
      181 cctgctggcg gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta
      241 ccgctcatgg tttggcagct atctacgttt ctggcagaaa catcatgcga tgccacgcgg
      301 cgtcaaaccg cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt
      361 ccagatgcca tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat
      421 gtggcgaatt ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt
      481 tgcaattatt gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca
      541 agggttaaac aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca
      601 agcaggcata cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc
      661 aaaagcattc aggactaccc aaaacccggc attcttttcc gagatgtcac cagcttactg
      721 gaagacccga aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg
      781 ggcattacca aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct
      841 ctgggtctgg gcgttggctt tgtgccggtc cgtaaaccgg gcaaactgcc gcgtgaaact
      901 atcagtgaaa cttacgatct ggaatacggc accgatcagc tggagatcca cgttgatgcc
      961 atcaaaccgg gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc
     1021 gaagcgaccg ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt
     1081 atcaacctgt tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac
     1141 agccttgtcc cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga
     1201 cagcacaaga tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt
     1261 cgtgaatcca ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa
     1321 aatggcgccc acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg
     1381 cgaacggctt gtcgttaggg cgtattcatc atgcttatct tttttccggt acccgtggcg
     1441 tcggaaaaac ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta
     1501 ccgcgacgcc gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg
     1561 atctgattga aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg
     1621 ataacgtcca gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc
     1681 atatgctgtc gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc
     1741 acgttaagtt tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc
     1801 gctgtttgca atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc
     1861 acatcctcaa cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg
     1921 ccgctgaagg cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg
     1981 acggccaggt ttcaacccag gcagtcagtg cgatgctggg tacgcttgac gacgatcagg
     2041 cgctgtcgct ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta
     2101 atgaagccgc tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt
     2161 tgcatcgtat tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca
     2221 tcgagctgcg gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact
     2281 atcagacgct gttgattggt cgcaaagaat taccgtatgc gccggaccgt cgcatgggcg
     2341 ttgagatgac gctgctgcgc gcgctggcat tccatccgcg tatgccgctg cctgagccag
     2401 aagtgccacg acagtccttt gcacccgtcg cgccaacggc agtaatgacg ccaacccagg
     2461 tgccgccgca accgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
     2521 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
     2581 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
     2641 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
     2701 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
     2761 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
     2821 tggcggcgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca caggtgagcc
     2881 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
     2941 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggtgcac
     3001 agcaaaaact ggctgaagcg ttgagcacgt taaaaggttc aacggttgaa ctgactatcg
     3061 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
     3121 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
     3181 ggttcttcga tgcggaactg gatgaagaaa gtatccgccc catttgatcg taagcacagc
     3241 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
     3301 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
     3361 atcgcgcagc tggaagtcac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
     3421 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
     3481 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
     3541 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
     3601 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
     3661 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
     3721 gcggcgggat gcgtctggcg caggcgctca cccgggccat gtcggaaatc ggccactgcg
     3781 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
     3841 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catttacgcc attgagcaga
     3901 cggggcagtt ttcaggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
     3961 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
     4021 ctgaagtgat cctcgccacc aaccccacgg ttgaaggtga agctaccgct aactacattg
     4081 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
     4141 gtggcgaact ggaaatggtc gatggcacca cgctgtcaca ctcccttgcc gggcgtcata
     4201 agattcgttt ttaagcaaac gagagcagga tcacctgctc tcgcttgaaa ttattctccc
     4261 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
     4321 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
     4381 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
     4441 tccgatgcgg cggacaagct gcgtttccgt gcgctctcta acccggacct gtacgagggt
     4501 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgtacgct gaccatctcc
     4561 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
     4621 tccggcacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga cagccagctg
     4681 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
     4741 cgtactcgcg cggcaggcga aaaaccagaa aatggcgtct tctgggaatc ggctggcgaa
     4801 ggtgaataca ccgttgccga tattaccaaa gaagatcgtg gtactgaaat caccctgcat
     4861 ctgcgtgaag gcgaagacga attcctcgat gactggcgcg tgcgttccat catcagcaaa
     4921 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
     4981 accgttatct cctgggaaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
     5041 gaaatcaccg atgaagagta caaagagttc tacaaacaca tcgcccacga ctttaacgat
     5101 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
     5161 attccgtccc aggctccgtg ggatatgtgg aaccgcgatc ataaacacgg cctgaaactg
     5221 tatgttcagc gtgtgttcat catggacgac gcagaacagt tcatgccgaa ctatctgcgc
     5281 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
     5341 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
     5401 ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
     5461 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
     5521 ctgcgttttg cttctaccca taccgattct tctgcgcaga ccgtatctct ggaagactac
     5581 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcaccgcaga cagctatgcg
     5641 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
     5701 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
     5761 ttccagtcgg tgtctaaagt tgacgagtcg cttgaaaaac tggctgacga agttgatgag
     5821 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgtgtgaa agccctgctc
     5881 ggcgagcgcg tgaaagatgt ccgtctcact caccgtctga ccgatacgcc agcgatcgtt
     5941 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
     6001 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
     6061 gcggcagata ctgaagatga agcgaagttc agcgagtggg tagaactgct gctggatcag
     6121 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaac
     6181 cagctgctgg tttcctgatg tcatgccgga tgaccttcgt gtcatccggc attttctttt
     6241 catcatctgc actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
     6301 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaat tcgacacatt
     6361 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
     6421 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
     6481 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
     6541 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
     6601 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
     6661 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
     6721 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
     6781 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
     6841 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
     6901 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
     6961 aagttgacgg caccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
     7021 aattcgaaag cgcgcacgga cagtcccctc gccccctcgg ggagagggtt agggtgaggg
     7081 gaacaggccc gcacaagcaa acttatcagc aatctcaggc cggatattca ttcggccttt
     7141 tacaaaaacc tcaatctgcg ctattccttt ttctgatttg acctctcaca gcaattagtt
     7201 cttcttcctc acttttccgc tacaattatc aacaagttga atcgataaga ggcggtaatg
     7261 cgtcagacta aaaccggtat cctgctggca aacctgggta cgcccgatgc ccccacacct
     7321 gaagcggtaa aacgctatct gaaacaattt ttaagcgaca gacgcgtggt tgatacctca
     7381 cggttgttat ggtggccgtt gctgcgcggc gtgattttgc cgctgcgctc gccgcgtgtg
     7441 gcgaagctgt acgcctcggt ctggatggaa gacggctcgc cgctgatggt ttacagccgc
     7501 cagcaacagc aggcgctggc acaacgttta ccggagacgc ccgtagcgct gggaatgagc
     7561 tacggctcgc catcactgga aagcgccgtc gatgaactcc tggcagagca tgtagatcat
     7621 attgtggtgc tgccgcttta tccgcaattc tcctgttcta cggtcggtgc ggtatgggat
     7681 gaactggcac gcattctggc gcgcaaacgt agcattccgg ggatatcgtt tattcgtgat
     7741 tacgccgata accacgatta cattaatgca ctggcgaaca gcgtacgcgc ttcttttgcc
     7801 aaacatggcg aaccggatct gctgctgctc tcttatcatg gcattcccca gcgttatgca
     7861 gatgaaggcg atgattaccc gcaacgttgc cgcacaacga ctcgcgaact ggcttccgca
     7921 ctggggatgg caccggaaaa agtgatgatg acctttcagt cgcgctttgg ccgggaaccc
     7981 tggctgatgc cttataccga cgaaacgctg aaaatgctcg gagaaaaagg cgtaggtcat
     8041 atacaggtga tgtgcccggg ctttgctgcg gattgtctgg agacgctgga agagattgcc
     8101 gagcaaaacc gtgaggtctt cctcggtgcc ggcgggaaaa aatatgaata tattccagcg
     8161 cttaatgcca cgccggaaca tatcgaaatg atggctaatc ttgttgccgc gtatcgctaa
     8221 agctgagcgg taaagaactg agcgccgtcg cgaagagcct cgtcggcggt tttcatcatt
     8281 cgtgaataat gcaaaaaggc atgcagcgta cctgggtaga gtttgaactc acagggctgc
     8341 tgatgcgccg ctaacgtctg gtaaagcaga cggctgtcat ccagcagcgg atcgaactcc
     8401 gccccggcaa taaaacaggg cggaacttcg cgagtgagat cattattaaa cagacagtaa
     8461 tacggtgact cgcggtccgc gtcgttgctt aaatacgcct cttcatacat ctgcaaatcc
     8521 tgttgcgtta agccatccca gacaccgccc aacagacgac gagtcacgga atcccgtaat
     8581 ccgtaaagcc cataccacaa cagaacagcg gcaacttttc cgcaatcaat ttgtttgtca
     8641 cgtagccaca aagcgctggc aagcgccagc attgcgcctg cagaatcacc ggcaaagcca
     8701 atgcgggaca tgttgatttg ataatctccc gcctgctggt ggaagtggca acaggcagcc
     8761 acaatttcct ctattgcctg cggaaaacgt gcttccggcg aaagagtgta atcaatacca
     8821 atcaccgtac attgggtgta atttgccagc agtcgcataa tgcgatcgtg gctatcaaga
     8881 ttgccgagaa taaagccgcc gccatgaaga taaaatagag tcgccgggct gtgacttttg
     8941 ggtgagtaga ggcgagtctc gacctgacca taaggtgttg gaaccatgta agcgctggtt
     9001 gccatttctg gagcgccagc attccagaat cggcgctcaa gcgtgtaata ctgtcgttgc
     9061 tcagcaatcg tccccgttgc gggccagagc ggtaaatccg gctgaatggt gtttacaact
     9121 gccttcattt cagcagaaat gagctccaga acgggtaatt tgttttccgg tttcataaaa
     9181 aactccttcc aaattacgcc attgtaataa gctgccacca gcaaaatgcg atcccgccgt
     9241 ccgatattga aagccgagtg tgtatcgcgc gttcaggtgt gaatgtgcta acattcgccg
     9301 ctcagttaat cacccataca aacaatcatg aaatttcccg gtaaacgtaa atccaaacat
     9361 tacttccccg ttaacgcacg tgatccgttg ttgcaacaga tccagcctga aaacgaaacc
     9421 agtgcctcct gggtggtggg tatcgaccag acgttggtag atattgaagc aaaagtggaa
     9481 gacgatttta ttaaacgtta tggattaagt gccgggcatt ctttggtgat tgaagatgat
     9541 gttgccgaag cgctttatca ggagctaaaa cagaaaaacc tgattaccca tcagtttgcg
     9601 ggtggcacca ttggtaacac catgcacaac tactccgtac tggcggacga ccgttcggtg
     9661 ctgctgggcg tcatgtgcag caatattgaa attggcagct atgcctatcg ttacctgtgt
     9721 aacacctcca gccgtactga tcttaactac ctacaaggcg tggatggccc gattggtcgt
     9781 tgctttacgc tgattggcga gtcaggggaa cgtacctttg ctatcagccc tgggcatatg
     9841 aaccagttgc gtgcggaaag tattccggaa gaagtgattg ccggggcgtc agcgttggtt
     9901 ttgacttcct atctggtgcg ttgcaagcca ggggaaccga tgccggaagc aaccatgaaa
     9961 gctatcgagt acgctaaaaa atacaacgtg ccggtggtac tgacactggg aaccaaattt
    10021 gttatcgcgg agaatccgca gtggtggcag gaattcctta aagagcatgt ttccattctg
    10081 gcgatgaacg aagaagaagc cgaatcgttg accggagaaa gtgatccgct gctggcatca
    10141 gataaagcgc tggattgggt ggatttagtg ttatgtaccg ccgggccaat cggcttatat
    10201 atggctggtt ttaccgaaga cgatgcaaaa cgtaaaactc aacatccatt gttgccaggt
    10261 gctatcccgg aatttaatca gtatgagttt agccgcgcca tgcgtcataa agattgtcaa
    10321 aacccactgc gcgtctattc gcacattgcg ccgtacatgg gcgggccgga aaaaatcatg
    10381 aacaccaacg gagcagggga tggcgcactg gcagcgttgc tgcatgacat taccgccaac
    10441 agctaccacc gcagtaacgt accaaactcc agcaaacata aattaacctg gttgacttat
    10501 tcgtctttag cgcaggtatg taaatatgct aaccgcgtca gctaccaggt gctgaatcag
    10561 cattcgccac gcttaacgcg cggtctgccg gagcgtgaag acagcctgga agaatcttac
    10621 tgggatcgtt aagttatcga ccagaccgga cgacgatggc atccgtaggc cggataagat
    10681 gcgctagcat cgcatccggc aataacgtct tgttttaccc cgtcaccacc tcacctgccg
    10741 gtggcgtttc cagcaattcc agcatagtac gggcaatttc acgctcaccc atcactacct
    10801 gattcgcacc acgttcggtg atataagcca cttcatcgtc ataatgggcg cgggcaataa
    10861 tctcaatatc cggatttttc gcgcgggcag atgccacaat ctcgcccgct tcataaccgt
    10921 tgggaatcgt caggatcagc catttagcac attccagatg cgccagttgc ataatttctt
    10981 cgttcgccgc attgcctaat accgcccgga ccccgcgctc tcgcagctca tcaacacggg
    11041 ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc gagcaatttc tcccccagca
    11101 ggctgcctac acgaccgtaa cccaccagta gcgcatggtt gcaaatatcc actgggatct
    11161 gcttctcttc ttcgattgcc tcttccagcg tctgctcttc cagcgtttcg gtcttcgcca
    11221 gatatttctc cagtagtgcg aacagtaccg ggttgagcat aatcgacagg atcgcccctg
    11281 ccagtaccag gttttgtccg gcctgcggca gtaaattcaa tgccattccc agtcccgcca
    11341 ggataaacgc gaactcacca atctgcgcca ggctggcggc gatggttaat gccgtacgtt
    11401 gggagtgacc aaacagtcgc accaggaaaa atgcggctaa cgacttacca aacagaataa
    11461 tcgccagcgt cgccagcact gccagcggtt gctgaatcag aattaacgga tcaaacaaca
    11521 tcccgacgga gacaaaaaac agcaccgcaa acgcgtcgcg caatggcagc gtatcgtggg
    11581 cggcacggtg actcagttca gactcgttca gtaccatccc ggcaaagaac gcaccgagtg
    11641 caaaggagac atcaaacagc tctaccgcac caaaggcaat ccctaacgcc agcgccagca
    11701 ccgacagggt aaacagctcg cgagaaccgg ttgccgcgct gcgtgccata atccacggca
    11761 ccagacggcg acctaccagc atcataatgg cgataaatgc gatcactttg ccgatggtga
    11821 tccccatatc gactgcaaga gtggcaaagc ccacatcgcc ctgttccatc attcctgcca
    11881 ctgcgggcag caacaccagc gtcagaacca tcaccaggtc ttccacaatc aaccaaccga
    11941 tggcgatttg cccacgctga ctgtctatta attgccgttc ttcaagtgcg cgcagtaaca
    12001 ccacggtact ggcggtggaa agacataagc cgaacacgat accggtcatt aacgaccagc
    12061 ccagcacggc ggagagcgcc atacccagca gcgtcgccac ggctatctgg gcgatcgcgc
    12121 cgggaatggc gatggacttt accgccatca aatccttgag cgaaaaatgc aaaccgacgc
    12181 cgaacatcag caaaataacg cccagttcag ccagttccgg cgcaagcttg gtatcggcaa
    12241 caaagcccgg agtgaatggt cctgccagca cacccgctaa cagatatccc accagaggag
    12301 aaatacgtag tttattggcc agcatgccga ggataaaggc gagcacaagg ccgccaacaa
    12361 tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc ttttcctggt ggttattgtc
    12421 catttttggc cgggaaaacc aaaattacag gtaatagttt atgacaattt cattgatgat
    12481 gttcatgaat aattattgaa ttttgcagaa aaatggaatt agctgcaaaa aaagcacgga
    12541 tcggaaaaca gaaggcggtt taacagagga aaggcttatg gcgactgtaa aacgatgcag
    12601 ccaaagtttg cctttggctg caatgaaatc agtctttatg ccggttatca ggcaggaata
    12661 tggtcaacat ccccaatagt ggcaggaaag cacagatttt atagactaac tcgatgctgg
    12721 tgtgatcggc gataagcccc agaactgccg ctcccagacc tcccatgcca aaagcaaaac
    12781 cgaaaaagag tccagaaacc ataccgatac gtcccggaag tagctcctga gcgtagacca
    12841 gaatggcaga gaatgccgaa gcgaggataa atccaataat caccgttaaa acccccgtcc
    12901 agtgcaggct ggcgtagggt aaaatcagcg taaacggcgc aacgccgagg atagagcccc
    12961 aaatcacata tttccgtcca attttatccc ctacaggccc gccgatcacc gtacctgccg
    13021 caacggcaaa caggaaggca aacagatgaa gctgggcatt ctggatagat aatccgaatt
    13081 tttgcatcag ataaaaggtg taatagctgc tgatgctcgc catatagaaa tatttcgaga
    13141 aaatgaggat taacagaatg ctgactgcca gtaccacttt gttgcgcggc agtggattga
    13201 ttatcgtcgc tttgggtttt cctttattca ttcggtgctg tgccgagtac caacggctaa
    13261 tttgcgccaa caccacgatc gccagcagtg ccgcaagcac aaaccaggca acgttgcctt
    13321 tgccataagg cgcgataatc accgccgcca gcaagggtcc cagggaactg ccaaagttgc
    13381 cgccgacctg aaagatagat tgcgccaggc catgccgccc gccggaagcc atacgggcta
    13441 cgcgagaaga ttccggatga aagaccgatg aaccggtacc gaccagcgcc gccgccagca
    13501 gaactgcgcc aaaactgccc gccagcgcaa gcagcaccag accacttaag gtaaagcaca
    13561 tgccaattgg caacgaccac ggcatcggat atttatcggt ccagtagccg accactggtt
    13621 gcagtagcga agaggcgagc tggaaggtga gggttatcat gccaatctgc ataaatgtca
    13681 gagaaaattc tgactgaagc agcggataaa tcgccagaat cagcgattgg atcatgtcgt
    13741 tcagcagatg tgagaggctg atagcaccta aaataccaaa cgatgttcgg gccttggtcg
    13801 ttgacgcagc cgcgcccgcc acaggctggg gttgttcact cattgccata ggaaagtcac
    13861 tttttcaggg ttgcgatgta aagaatgatc ttatttgtga ttattacccg actaacatac
    13921 ctgtatgcgt cgtctgaagg aagtctcaac gccgaataca gaatttctaa tctggatgca
    13981 gatttatctt caccggacgc agacttatct atgatgtcgc gtcatactat ttttcaacac
    14041 gttgaaatca ggtcagggag agaagtatga aattattgca gcggggcgtg gcgttagcgc
    14101 tgttaaccac atttacactg gcgagtgaaa ctgctctggc gtatgagcag gataaaacct
    14161 acaaaattac agttctgcat accaatgatc atcatgggca tttttggcgc aatgaatatg
    14221 gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg tatccgcaaa gaggttgcgg
    14281 ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat taacactggc gtgcccgagt
    14341 ctgacttaca ggatgccgaa cctgattttc gcggtatgaa tctggtgggc tatgacgcga
    14401 tggcgatcgg taatcatgaa tttgataatc cgctcaccgt attacgccag caggaaaagt
    14461 gggccaagtt cccgttgctt tccgcgaata tctaccagaa aagtactggc gagcgcctgt
    14521 ttaaaccgtg ggcgctgttt aagcgtcagg atctgaaaat tgccgttatt gggctgacaa
    14581 ccgatgacac agcaaaaatt ggtaacccgg aatacttcac tgatatcgaa tttcgtaagc
    14641 ccgccgatga agcgaagctg gtgattcagg agctgcaaca gacagaaaag ccagacatta
    14701 ttatcgcggc gacccatatg gggcattacg ataatggtga gcacggctct aacgcaccgg
    14761 gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct ggcgatgatc gtcggtggtc
    14821 actcgcaaga tccggtctgc atggcggcag aaaacaaaaa acaggtcgat tacgtgccgg
    14881 gtacgccatg caaaccagat caacaaaacg gcatctggat tgtgcaggcg catgagtggg
    14941 gcaaatacgt gggacgggct gattttgagt ttcgtaatgg cgaaatgaaa atggttaact
    15001 accagctgat tccggtgaac ctgaagaaga aagtgacctg ggaagacggg aaaagcgagc
    15061 gcgtgctgta cacccctgaa atcgctgaaa accagcaaat gatctcgctg ttatcgccgt
    15121 tccagaacaa aggcaaagcg cagttggaag tgaaaatagg cgaaaccaat ggtcgtctgg
    15181 aaggcgatcg tgacaaagtg cgttttgtac agaccaatat ggggcggttg attctggcag
    15241 cccaaatgga tcgcactggt gccgactttg cggtgatgag tggaggagga attcgtgatt
    15301 ctatcgaagc aggcgatatc agctataaaa acgtactgaa agtgcagcca ttcggcaatg
    15361 tggtggtgta tgccgacatg accggtaaag aggtgattga ttacctgacc gccgtcgcgc
    15421 agatgaagcc agattcaggt gcctacccgc aatttgccaa cgttagcttt gtggcgaaag
    15481 acggcaaact gaacgacctt aaaatcaaag gcgaaccggt cgatccagcg aaaacttacc
    15541 gtatggcgac attaaacttc aatgccaccg gcggtgatgg atatccgcgc cttgataaca
    15601 aaccgggcta tgtgaatacc ggctttattg atgccgaagt gctgaaagcg tatatccaga
    15661 aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg tgaggtgagc tggcagtaat
    15721 ccgaaagtgc cggatgtttg catccggcac aatgcttaat cgcggcgggc gatatcagca
    15781 aatttggcat cgaggatctt tgccagatcg cctgccgcca gttcgatatc cagtccgcgc
    15841 ttgccgccgg aaacataaat agtggcaaat tcttgtgcgg gggcgtcgat aatcgttggc
    15901 agacgttttt tctgccccag tgggctaatc cctccaacca ggtatcccgt cgaacgctgc
    15961 gcgaccatcg gatcggccat ctcaactttc ttggcaccca gcgcttttgc tactttttta
    16021 agatccagtt gacctgcgac cggcgtaacg gccacggcaa ggtgtttcat atcaccgttc
    16081 actgccacca gcagcgtttt gtagacctga tccggattca aacctaattt tttgacgact
    16141 tcatcgccaa aattggtttc agccggatcg tgctcgtagg tatggatttg aaacgaaatc
    16201 ttgttttttt cgagtaattt aactgcgggt gtcatggtaa ttttcctgcc ttaaacaaaa
    16261 aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa gagtcatctt gcctaagagt
    16321 attggcagga tggtgagatt gagcgacaat cgagttacac cgtcgcttaa agtgacggca
    16381 taataataaa aaaatgaaat tcctctttga cgggccaata gcgatattgg ccattttttt
    16441 agcgcaacat ttgcggcaaa ttcccttctc catacaggtg tagtgcaccg accgcgacca
    16501 catatcgccc cggcggcatg gcgcgtaatt tatcccgcca ggcgagattt cgctgatgca
    16561 tcagcacatc gtacagcgac tgactgaacg tattgggcag cgttatatca ttattttgcg
    16621 gcggtgcatt cagccaccag ctcatcattt gttgcagcaa ccgtgcgttg gtatgccagt
    16681 gggtcagcgt atcgtccagc agcgccagtc ctttgtcagg gagctggagc aacatggcaa
    16741 tctggttttc agccccttcc agttcaatca cgggtttatg ttgttgcttc gccgcctgca
    16801 atagctggta atcgataccg tattctgccc gcagccccag tttttgtgcc tgcgtcgcct
    16861 gaagaaccat cgcgatttgc cacagcggtt gggtagaaaa gagcgaggga gaaatgccca
    16921 tctcctggct aatgtgctgc aggttttgga gttgctcctc gctaatgcgc tcttccagcg
    16981 cctcgcaggc aggcaaatta gcaaaaggcg tatcactggt ggaaacatcc gcctcgacga
    17041 tcagcgcatc ggcgtttttg agctttttga gcaaacgggt gggcagggga gccatatcgt
    17101 ggctacccat atgaatactg ccaatcagat gaaaatggcg attgccgggg agggtgatat
    17161 cgatggcagg ccaggtgtaa tgattaccgc gcagcgcggc ccaaagcgtt tttacccggt
    17221 acaacagatc catacgacct ccctttgtga aatatcatgc tagcgcgcgg tgagggatgg
    17281 cgcaaatgga gttggggact tgtttaatgt gtttgtatga ttcagtatgt tcttgcatcg
    17341 ctattcacaa ggaagcaaca gttaaaaacc atgaaacagg caacaagaaa accgacgaca
    17401 cccggagata ttcttctcta tgaatatctg gaaccgctcg atttgaaaat caatgagtta
    17461 gcagagttgc tgcatgttca tcgtaatagc gtcagtgcac tgatcaataa caatcgtaaa
    17521 ctcaccactg agatggcatt ccgtctggcg aaagtttttg ataccacagt cgattttagg
    17581 ctaaacctcc aggcggcagt tgatctttgg gaagttgaaa acaacatgcg cacccaggaa
    17641 gaattgggac ggattgaaac agtggctgaa tatttggcac gccgtgaaga gcgtgcaaaa
    17701 aaggtcgcgt aaggccaaaa catcgcttca tctcgtaggc cggataaggc gttcacgccg
    17761 catccggcat ccgagcacca ttgcctgatg cgacgcttac ccgtcttatc aggcctacaa
    17821 acctgtgcct gaaccgtagg ccggataagg cgtttacgcc gcatccggca gtcatgcgtc
    17881 gatgccaaat gcgccgccct aaagcagcgc atccgcaatg acgtacttat tccttcggtt
    17941 taaaccgcag caaccggttg gcgttactca ctacggtaat cgacgagagc gccattgccg
    18001 ctccggcaac taccgggtta agcagtgttc cagtaaacgg ccacaaaata ccggcggcga
    18061 ccggaatacc gatgctgttg tagataaagg caccgagcag gttctgcttc atgttgcgca
    18121 gcgttgcgcg ggaaatcgcg agcgcatccg caacgcccat caggctatgg cgcatcaggg
    18181 taatcgccgc ggtttcaatg gcaacatcac tgccgccacc catcgcaatg ccgacatccg
    18241 cctgggccag cgctggcgcg tcgttaatgc cgtcgcccac cattgccacc tgacgtcctt
    18301 cactttgcag acgtttgatc gcttcggctt taccgtccgg cagcactccg gcaatcactt
    18361 catcaattcc agcttctttg gcgatcgcat tggcggtggt tgggttatcc ccggtcaaca
    18421 tcaccagacg atatcccgct ttatgcaggc gttgcagcgc cgccacgcta tcactacgca
    18481 acggatcgcg tactgccagc agggctaccg ctttcccgtc aaccgccagc agcacaggcg
    18541 ttgccccttg cgatgcctga gcagtaatct ccgcttcgat agctttggta ccaacctgtt
    18601 gctcatttaa cagcgcctga ttgcccagca ataacgcatg accttcagct tcaccgctca
    18661 cgcccagccc gcgcaatgtg cggaaaccgt tgacctgcgg cagctgcata tcacctgctt
    18721 tatcgaggat cgcccgtgcc agcggatggc tggaaccttg ctccagtgcc gccgccagac
    18781 gcaatgcctg cgcttcatca acatcagcaa acgttttcac tgcgacaacc tgcggcttcc
    18841 cttcggtcag cgtcccggtt ttatcgaaca cgacggtatc gagtgtactg gcgcgttgca
    18901 gcgcgtcggc gtcccgcacc agcacgccaa actcagccgc ccgcccgacg ccggaaataa
    18961 tcgacatcgg cgtcgccagc cccagcgcac acggacaggc gataatcagt accgtggtgg
    19021 caatcaccag ggtataaaca atttgcggtg ccggaccaaa gaagtaccag attgccgcac
    19081 tgaccagcgc aatcaccacc accacaggta caaatacggc tgagatttta tccgccagct
    19141 gaccgatttc tggcttgctg ctctgggcct ggcgcaccat gcgaatgatc cgtgacagcg
    19201 tggtatggct gccaaccgca ctggcacgaa acagcacact gccgtcctgt accactgtcc
    19261 cggcatggac gctttcgcct tcgccttttt gctgtgggat tggttcgccc gtcagcatcg
    19321 cttcatccag ccatgcttcg ccctgggtaa tctcgccatc taccggcacg cgatcgccgg
    19381 gcgtcaggcg cagtaacatc cctggctgaa cttccgccag cggcacgctt ttttcaccct
    19441 ctgcggtgac cactcgcgcc gtcggtgggg ttaaatcgag taatttctcc agcgccttag
    19501 aagaacgctg acgtgcgcgc gcttccagca tatggccgag attaatcaga ccgataatca
    19561 tcgcactggc ttcatagtaa agatgccgcg cttccatcgg gaaccactgc ggccacaggt
    19621 tgacgctcat cgaatagagc cacgccacac ccgttcccag cgccaccagc gtatccatcg
    19681 tcgctgcacc gttcagcagg cttttccatg cactgcggta aaaatggccg ccggcgaaaa
    19741 ccatcactgc cagggttatc aggccgataa ctaaccacaa gctgcggttg tcagcggtga
    19801 ccatcatgtt atcgccgatc atcccccaga ccattaccgg gatacccaca gccagtgcaa
    19861 caattgcctg ccagcggaag cgcttcatcg tagctacggc ggtttcttgc tggcgctcgc
    19921 ggcgtttagc gtcatcttca atcgcttccg caccgtagcc cgctttttcc accgcctgca
    19981 ctaaatcttg tggggaggca ctgcccatca ccagcgcagt acgctccgcc aggtttaccc
    20041 gtgcctgagt gacgcccggt acgctttgca gcgcattttg tacgcgggtg acacagctgg
    20101 cgcagctcat gccgctcagc agcaactgct ggctgtcatc gtcatcggcg gtcgctgccg
    20161 gaagcgcctc agaaaccgct gtcagtgctt ccgacgggat tgatgactcc gccagcggtt
    20221 tagcctttgg gtggcttaca gatgcgtcat aacccgcttg tttgatggtt tcgatcagct
    20281 gttctgcact ggcagtcccg gtaacgtgcg cttcagtgat agacacatcc gcctgctcaa
    20341 catccgggcg ctgttcaaga ctttctttca cgcgtttaac gcagtgaccg caggacaggc
    20401 cgtccagggt caggtcgata gtttgtgaca taaaacactc ctttaagaca gttttgactg
    20461 gctgtgataa aggttaaacc ttccagcaag gggaaggtca agaaattaat aaaccaggcg
    20521 agtaaaagtc cgtaaagatt aaaaaattgg ctcaatttgc atcaggatta gacatttatc
    20581 tctttgtttt cctgtagtta agttgcgggt gctaagttaa atcaggatgc ctgaaaatcg
    20641 gcaccggggt gaggaattac ctcccgcatc tataaaaagg agttaacaaa agatgttaga
    20701 tgcaaacaaa ttacagcagg cagtggatca ggcttacacc caatttcact cacttaacgg
    20761 cggacaaaat gccgactaca ttccctttct ggcgaatgta cctggtcaac tggcagcagt
    20821 ggctattgtg acctgcgatg gcaacatcta tcgcgcgggt gatagtgatt accgctttgc
    20881 gctggaatcc atctcgaaag tctgtacgtt agcccttgcg ttagaagatg tcggcccgca
    20941 ggcggtacag gacaaaattg gcgctgaccc gaccggattg ccctttaact cagttatcgc
    21001 cttagagttg catggcggca aaccactgtc gccactggta aatgctggcg ctattgccac
    21061 caccagcctg attaacgctg aaaatattga acaacgctgg cagcgaattt tacatatcca
    21121 acagcaactg gctggtgagc aggtagcgct ctctgatgaa gtcaaccagt cggaacaaac
    21181 aaccaacttc cataaccggg ccattgcctg gctgctgtac tccgccggat atctctattg
    21241 tgatgcaatg gaagcctgtg acgtgtacac ccgtcagtgc tccacgctca tcaatactgt
    21301 tgaactggca acgcttggcg cgacgctggc ggagggtggt gtgaatccgc tgacgcataa
    21361 acgcgttctt caggccgaca acgtgccgta cattctggcc gaaatgatga tggaagggct
    21421 gtatggtcgc tccggtgact gggcgtatcg cgttggttta ccgggcaaaa gcggtgtagg
    21481 tggcggtatt ctggcggtcg tccctggcgt gatgggaatt gccgcgttct caccaccgct
    21541 ggacgaagaa ggcaacagtg ttcgcggtca aaaaatggtg gcatcggtcg ctaagcaact
    21601 cggctataac gtgtttaagg gctgatcatg atgaacacgg aaggtaataa cggtaacaaa
    21661 cctctcggt
//
LOCUS       DAOFQD010000076        21254 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.076, whole genome shotgun sequence.
ACCESSION   DAOFQD010000076 DAOFQD010000000
VERSION     DAOFQD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.076"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(112..513)
                     /gene="nikR"
                     /locus_tag="QBF46_002969"
     CDS             complement(112..513)
                     /gene="nikR"
                     /locus_tag="QBF46_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDQ6675126.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(519..1325)
                     /gene="nikE"
                     /locus_tag="QBF46_002970"
     CDS             complement(519..1325)
                     /gene="nikE"
                     /locus_tag="QBF46_002970"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDQ6675127.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1322..2086)
                     /gene="nikD"
                     /locus_tag="QBF46_002971"
     CDS             complement(1322..2086)
                     /gene="nikD"
                     /locus_tag="QBF46_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDQ6675128.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2086..2919)
                     /gene="nikC"
                     /locus_tag="QBF46_002972"
     CDS             complement(2086..2919)
                     /gene="nikC"
                     /locus_tag="QBF46_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDQ6675129.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(2916..3860)
                     /gene="nikB"
                     /locus_tag="QBF46_002973"
     CDS             complement(2916..3860)
                     /gene="nikB"
                     /locus_tag="QBF46_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDQ6675130.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3860..5434)
                     /gene="nikA"
                     /locus_tag="QBF46_002974"
     CDS             complement(3860..5434)
                     /gene="nikA"
                     /locus_tag="QBF46_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDQ6675131.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5545..6132)
                     /gene="acpT"
                     /locus_tag="QBF46_002975"
     CDS             complement(5545..6132)
                     /gene="acpT"
                     /locus_tag="QBF46_002975"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDQ6675132.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTAESVQWIDSVN"
     gene            complement(6187..7236)
                     /locus_tag="QBF46_002976"
     CDS             complement(6187..7236)
                     /locus_tag="QBF46_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDQ6675133.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7368..8585
                     /locus_tag="QBF46_002977"
     CDS             7368..8585
                     /locus_tag="QBF46_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6675134.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8589..9146)
                     /gene="dcrB"
                     /locus_tag="QBF46_002978"
     CDS             complement(8589..9146)
                     /gene="dcrB"
                     /locus_tag="QBF46_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDQ6675135.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9219..9884)
                     /locus_tag="QBF46_002979"
     CDS             complement(9219..9884)
                     /locus_tag="QBF46_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDQ6675136.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10105..10350
                     /gene="tusA"
                     /locus_tag="QBF46_002980"
     CDS             10105..10350
                     /gene="tusA"
                     /locus_tag="QBF46_002980"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDQ6675137.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10452..12650)
                     /gene="zntA"
                     /locus_tag="QBF46_002981"
     CDS             complement(10452..12650)
                     /gene="zntA"
                     /locus_tag="QBF46_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDQ6675138.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(12724..13350)
                     /locus_tag="QBF46_002982"
     CDS             complement(12724..13350)
                     /locus_tag="QBF46_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDQ6675139.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13491..13850
                     /locus_tag="QBF46_002983"
     CDS             13491..13850
                     /locus_tag="QBF46_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDQ6675140.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(13853..14122)
                     /locus_tag="QBF46_002984"
     CDS             complement(13853..14122)
                     /locus_tag="QBF46_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDQ6675141.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKTTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14112..14708)
                     /gene="rsmD"
                     /locus_tag="QBF46_002985"
     CDS             complement(14112..14708)
                     /gene="rsmD"
                     /locus_tag="QBF46_002985"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDQ6675142.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAEATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            14858..16351
                     /gene="ftsY"
                     /locus_tag="QBF46_002986"
     CDS             14858..16351
                     /gene="ftsY"
                     /locus_tag="QBF46_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDQ6675143.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16354..17022
                     /gene="ftsE"
                     /locus_tag="QBF46_002987"
     CDS             16354..17022
                     /gene="ftsE"
                     /locus_tag="QBF46_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDQ6675144.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17015..18073
                     /gene="ftsX"
                     /locus_tag="QBF46_002988"
     CDS             17015..18073
                     /gene="ftsX"
                     /locus_tag="QBF46_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDQ6675145.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18318..19172
                     /gene="rpoH"
                     /locus_tag="QBF46_002989"
     CDS             18318..19172
                     /gene="rpoH"
                     /locus_tag="QBF46_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDQ6675146.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19443..20546
                     /gene="livJ"
                     /locus_tag="QBF46_002990"
     CDS             19443..20546
                     /gene="livJ"
                     /locus_tag="QBF46_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDQ6675147.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARSYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(20745..21128)
                     /gene="panM"
                     /locus_tag="QBF46_002991"
     CDS             complement(20745..21128)
                     /gene="panM"
                     /locus_tag="QBF46_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDQ6675148.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5030 a         5604 c         5884 g         4736 t
ORIGIN      
        1 ggcgtacatt acgcagaata tctgccttta caggatctat ctatgcaact gaacttaatt
       61 ttataaatta agttcagtta atatcttttc tttcctcgtt tcagaaataa ctcaatcttc
      121 cttcggcaag cactgcaaat gcccgtgccg cacgccgcgc tgggcgataa cgtcatcggc
      181 aaaatgctgc acgtcaccca tgtcaccttt caacacggcg atttccagac agtcgtcgtg
      241 gttgatgtgc acatgcagcg tggcgacgga gaggtcgtgg tgatgatgct gggtggaaac
      301 aatacggctg gctaagtcgc gtttttcgtg ttcatacaca tacgacagca ccgcgaaacc
      361 ttgtgtgccg tgctgctggg tagcttcttg cgccagggcg ctacgcagaa tatcgcggat
      421 agcttcggaa cggttgttat aaccacgacg ctggctcagg ctgtccagcg tctccagtaa
      481 atcgtcatca agcgtgatgg tgactcgttg catttgcgtt aaaccttttc tgtggtgcga
      541 cggcgcacgg ggaatgcggg taataccgcg ttttgtagca cacgtccggc gtcagaggaa
      601 aaggttaatt tatctcccac cacctgggtt tcgacgattt gtccgttgtc catcaccatc
      661 acccgctgac agaagcgttc caccaggcgt aagtcgtggg tgatgaacag gcaggcggtg
      721 ccaaactgtt gttgtagctt tttcagcagg cgaatgacgc ccgcctgtaa cacgagatca
      781 aggttagaaa cggcttcatc cagaatcagt agtttcggtt cgaccgccag cgcgcgagcc
      841 aggcagacgc gctggagctg gccgccgctt aactgcggtg ggcgtttgtc gagaacgctg
      901 tcatcgagat cgaccgcctt cagcatttcg ctggcgcgcg ccagttgttc ggattttttc
      961 agtgatagca ggtggcgcat cggttcacgc aggatctcgc gcacggtttt gcgcggattc
     1021 acggcgctga tggagtcctg aaataccatc tgaatatcgc ggcggaacgc tttacgctgg
     1081 gcgcgattga gtttcgccag cggttcgccg cgccagctaa tattcccttg tgagggagac
     1141 tctaagccta ccagcagccg cgcgagggtg ctttttccgc agccgctgcg tcccagcagg
     1201 gcgacggttt cgccgctttt cagggtcagg gaaacgttat tcagcaccgc ctgatgttga
     1261 tgttttccgc taaatccacc gtgggcatag tgatgggaaa ggccgcagac gttaagtaag
     1321 gtcatgatgc cagctccata ccgtagaggg cgagatgagc ggaaaccagg ctgcgcgtca
     1381 ccgtatgttt gggggcgtta aacagcgttt ctacatcgcc ctgttcgaca attttacctt
     1441 gtgacatcac cgccacgtca tccgccagac gcgccacaac gcccatatca tgggtgacca
     1501 gcagcattcc cggcgcttgt ttttgcataa tgctttccag cagatcgagg atgcgcgcct
     1561 gtgctaccac gtcgaggtcg gtggttggtt catcggcgat gataaacggt gattcacaca
     1621 gcaccgccat cgcaatcatc atgcgctgca acatgccgcc gctcatctcg aacgggtaca
     1681 gcttcagcac gcgcgcggcg ttttccagcc ccaccgcttc tatggcagcg gtaagcgtag
     1741 cgtcatcggc gggtttccct aacgccaggc aggtttcccg cgcgtgggtg tgcatggtgt
     1801 gcagtggatt aaaggcgctg cgcgggttct gcatgatggt ggcaattttg atgccgcgca
     1861 gggcgcaagg cgaaaccggt ttgccatcgg ctaaaatttc ccccgccgtc tggcgaacgc
     1921 cagcgggcaa aatgcccagc gtcgcggcgc aggttaatga tttcccgctg ccgctaccgc
     1981 cgactaacgc cagcacgcgc ccgcgttgca gggttaacga tacaccgtgt accagcggct
     2041 gcgcggcctg tagcgcgata ttacgtagtt caatctgttg cggcattagt gtgcgtgctc
     2101 cgtcaccaga tgagggtcca gatgatcgcg cagtgcgtca cccaccaggt taaaggccat
     2161 cacgctgata aacagcgcca gccccggcca gaacatttgc agcggctggg tccagatata
     2221 ctggcgcgcg tcgttaatca tcacgcccca ttcggcggtc ggcgcggtca caccgaggcc
     2281 aaggaaagac atccccgcga cgtgcagcat catatggccg atatccagcg ttgccagcac
     2341 cagcagcgaa gggatcaccg cgcctgccag atgatcgaca aacacccgca catggcccgc
     2401 gccagaaagc cgtgacgcca gcacaaactc gcgttggcgt agtgaaatca ccaggctgcg
     2461 caccatgcgt gcataccacg cccagtgcga cagggcgatg gcgataatta cgttggtcag
     2521 cccggtgccg agcacgccaa ccataaagaa cgacagaatc gaggtcggga aggtcataaa
     2581 catatcggcg acgcgcatgg tggcctgatc aacgcgcccg ccaatcaacc cggcgctgcc
     2641 gccaataacc agccctaatg tcagcaccag cagcaggcag gccattaccg aaccgagcga
     2701 cacgcgggtc gctgccatca gccgcgagaa aatatcgcga cctaagtgat cggtgcccag
     2761 ccagtgctgc gcatccggcg aaagcaggcg cgacggcaaa tcaatcgcct gggggtcata
     2821 cggcagccac cactggctgg tgagtgcaat cagcgccagc agggcgataa tgatcagcgc
     2881 caggcgtacc gaccagcggg aagagaggaa aaagttcacg cgtgcgctcc ttcatgacga
     2941 cgaatgcgcg ggtccagcgc ggcgttgagc aaatcgacaa tcaaattaca gaccacaaaa
     3001 accaccacca tcatcagcgt aaagcactga atcaccggat agtcacggtt aaaaatcgcc
     3061 gacaccgcat agcgcccgac gccaggccag gcaaagatgt tttcgataat catcgtcccg
     3121 ccaatcaatt cgccaatgtg catccccacg gcggtgatca tcggcagcga ggcattgcgc
     3181 agaatatggc ggcgttcggt ctgtttgtca ttcaggccgc gtagccgcgc ccaggtgacg
     3241 tgacgctgac cggcgacgtc cagcatactg gcgcgcagca gtcgcgcgtt aatcgccagc
     3301 gacataaagg cgatggaaac cgcaggcaaa atgatgtgct gccagccgcc gtagcccatc
     3361 gcgggtagcc attgcagata caccgaaaac gccatcacta gcaaaaatgc cagccagaag
     3421 ttgggcatcg acacgccaag aaacgcgata aagcgcacgg cgaaatccgg caaacgatcg
     3481 cggtggcgcg ccgcccagat accgagcggt acggaagtga gcagaattaa taccagcgcc
     3541 gcacctgcaa gttccagcgt ggcgggcagg aagttcagca tatcgtccag taccgggcgt
     3601 tggctggcga atgagatacc aaagtcaaga tgcagcgcct tccacaacca ggtaccgtac
     3661 tggacgtaca gcggctgatc cagcccgagc atggtgcggg tagaggccag catctccggc
     3721 gtcggcggca ggttagacag acgcaaataa tcgagcgccg ggtcgccggt accgaggcgc
     3781 agcatcagaa aaatgatcac cgaggcggcg agcaccatcg ggatcagcag cagaaagcgg
     3841 cgtaatacgt aacgcaacat taaggtttca ccggtttaat ctgttcgaac ggaatttcgg
     3901 tggcgatcgg cgcgtagggg atattaccca gctccggttt tgataccacc atcatcgaga
     3961 tgtagctgat aggcagataa accgcctcgt catgcagacg ggtcagaatg tcgcgataca
     4021 gcgcctgacg ttgcgtttcg tcatgggtcg ccagcacttc gccgatctct ttatcaatca
     4081 gcggtttgtc ggctaatcct tgctgtgcct ggaagtcagc gtgtgacggt acgcgcattg
     4141 aactgaggaa ggcgtgtgga tcatatggcg cgccccaggt gcggtggaaa atcatgccaa
     4201 aacgaccgtc gcgctgacga gcatagatac tgctctcttc ttcgccaatc agcgagacat
     4261 ctgcgccaat ctggcgcata tcagcctgaa tgatttccgc catcgatttg cttaacgcat
     4321 cggtgccgat gaacgaaagt tcaatgcgca gcggctgacc atttttctcg cggatgtctt
     4381 tgcccgcagg cagcgtccaa ccggcttttt ccagcaacgc tttcgctttt tgcggatcgt
     4441 actggcgcgg tttcaggccg aggttggcgt agggcacaga aggggcaaac agggtgtcgg
     4501 cgacctgctg ggtgccatac aacgcgttat caatcagcga ttttttgttt accgcgtaat
     4561 taagagcttc acgtactgcc agctcgttgg tgggggcttt ggcggtattg agcgccagca
     4621 tcacggtttc gatcggctgt gacagttggg tgtggtaagc cggattctgg ctaaagcggg
     4681 cgaaggtatc gagcggtaat aacccttcgt ttccgtacag caggtcgata tcgccagttt
     4741 caaacgccac cgcgcgggta gtcgggtccg ggatgacgtt aaaggtgatc tttttaatcg
     4801 ctggcttttc gccccagtag ttttcgttac ggacgaagac atcgtactga ttcagtttcg
     4861 attcctgcaa aatccacggt ccggtgccaa tcggcgcttt aattccgttc atggtttcat
     4921 ggtttttaaa ctgcgaggga gcgataaaac ggaaaggacg gggcagggcc agttcttgca
     4981 ggaaaggata gtaggcgctt ttcagggtaa tttgcagctc tgttttactg agtgctttaa
     5041 catcaacaat ctggtttgcc agctccagcc aggcgtgacg ttgacggtta tcgagcactg
     5101 cgcggaagtt ttctgccgcc gcctcggcat cgaacggttc accgttggag aatttcacgt
     5161 catcacgcag ggtgaaggtc caggttttac catcttctga atgagtccag ctttttgcca
     5221 gccacgggat caccgaaccg tctgcctgat atttcaccaa tggttcataa accatgctct
     5281 gggcgaacat ctggttaggc gtgtaaaggt gcgggtttag tggcccgaca ttcaccggcc
     5341 aggcggtggt gatttcatct ggtgcagcgg catggacgat aaaagacgca caagccagca
     5401 gcgcaaatag agtgcggcgg agtgtggaga gcatggtaac cccaatggat taaaatagat
     5461 ggcggaaata agtatgacga ttttaagtat tcgtcatact gattacctgt tctggatcaa
     5521 tagatgggca gtcgggtggg cggatcagtt aactgaatcg atccattgca cactctcggc
     5581 ggtgagcgta aagggggtgg gggtgcagat cgcaaggctt aaattttcta gctgacaatg
     5641 gctgactgac agcgaggagt gataggtgct atctacgctg acgatttgcc aggcgctgcc
     5701 gccacgctgt ttaacgatgg cttctttgcg cgtccagatg cgccagaaca tttccagttg
     5761 ctgatcagga tgcacggcgt ccatttcagc gtgttccccg aggctgaaca cggcgttcgc
     5821 cagccagcgc cagttggcgc gcgggcgaat cacttcgata tcgcagccga cttcgccttc
     5881 atcgctcaac agcagagcga tatcgtcacc gctatggctt aagttgaacc atagcggcgt
     5941 ttccggcgcg aatgcaggtt tgccttgttc gccatagatg atctccggta gcggggaaag
     6001 cgtgtgcgaa agcaatgcac gccccgccag ccagcgttcg cgtcgtggac cttgcggtgc
     6061 ttgctcgcgt aaacccggtg gcagtggagc tgcgcttaag gtcgaaactt tccccagaac
     6121 tatccgatac atatcagggc caacgtttaa tggaaaatga aagtgcgtat cgtatcactt
     6181 gtcgcctcat cccggtaacc gacttttcgg tctgcctggc cccagtaaaa tcgccagttt
     6241 gctaccgcct ttggtggttt ccatccagat tttacacaca ctggttaatg gcaccgaaag
     6301 tagcatccct accgggccga gcagccatcc ccaaattaac aatgaaagaa ataccaccat
     6361 ggtggacatc cccaggcgat ggcccatcat ccgtggctct aaaatattgc cgatgaccat
     6421 atggacaact aaaaacaatg cgccgaccag aatacattcg taaataccat taaacagcag
     6481 cacctgaatc attggcggta cggcggaaat taccgcgccg atattgggca cgtagttaag
     6541 caaaaacgcc agtactgccc acatcagcgc aaactgtacc cccatcagct ccagccccag
     6601 ccagacgatg acacctgtcc ataaactgag tagcgtcttc aatgcaagat agtgcgaaac
     6661 gcctttaagt gcgcggtgta atcccgcgat gtgaatctgt ggattattca gcgcaaaacg
     6721 cattttgtaa gggacgtggc gcacttcaaa cagcataaaa actacggtca tcaccagcaa
     6781 aagcacgctc gccattgccc cggaaagccc ggtcattagc gccgtggtga aggtaaccac
     6841 tttttccgag tccatccgct gcagcattcg ctccggcgac atatgcaaat taagaaaagg
     6901 caacatctcc tgcaatttaa aaagtttgcg cgtcagctcc ttattaaact tcggcagcat
     6961 agagataaat tcgttaaacg atgccgccag tacgccgacc agcgcggtta gtgcgatcag
     7021 catcaccacc actacaatcg taatggcaac ggggcgttgt actccccgac gaataaacca
     7081 ggtaacgagc gggttgagga cgatggcaaa aaacagcgcc agtaacagct gcacaatgat
     7141 atctgccgct gcgtgaatgc ccgcgaggat cactaccagc gaggccagct tgagcagaat
     7201 gtgcatgccc gttttatcgg gttgagggat ttccattggg gcttccttgt gactttttgt
     7261 attaagtgta gcgggagtca cgccagcaat attctgattc ttactgctga ttttcacgtc
     7321 agcacgtggt aaaaatgaaa cactgttgta aaaatgtggt gatcctcatg cccgaacccg
     7381 tagccgaacc cgcgctaaac ggattgcgcc tgaatttgcg cattgtctcc attgtcatgt
     7441 ttaacttcgc cagctacctc accatcgggt tgccgctcgc tgtattaccg ggctatgtcc
     7501 atgatgtgat gggctttagc gctttctggg caggattggt tatcagcctg caatatttcg
     7561 ccaccttgct gagccgtcct catgccggac gttacgccga tttgctgggc cccaaaaaga
     7621 ttgtcgtctt cggtttatgc ggctgctttt tgagcggtct gggatatctg acggcaggat
     7681 taaccgccag tctgcccgtc atcagcctgt tattactttg cctgggacgc gtgatccttg
     7741 ggattgggca aagttttgcc ggaacgggat cgaccctgtg gggcgttggc gtggttggct
     7801 cgctgcatat cgggcgagtg atttcgtgga acggcattgt cacttacggg gcgatggcga
     7861 tgggtgcgcc gttaggcgtc gtgttttatc actggggcgg cttgcaggcg ttagcgttaa
     7921 tcattatggg cgtggcgctg gtggccattt tgttggcgat cccgcgtccg acggtaaaag
     7981 ccagtaaagg caaaccgctg ccgtttcgcg cggtgcttgg gcgcgtctgg ctgtacggta
     8041 tggcactggc actggcttct gccggatttg gcgtcatcgc cacctttatc acgctgtttt
     8101 atgacgctaa aggttgggac ggtgcggctt tcgcgctgac gctgtttagc tgtgcgtttg
     8161 tcggtacgcg tttgttattc cctaacggca ttaaccgtat cggcggctta aacgtagcga
     8221 tgatttgctt tagcgttgag ataatcggcc tgctactggt tggcgtggcg actatgccgt
     8281 ggatggcgaa aatcggcgtc ttactggcgg gggcagggtt ttcgctggtg ttcccggcat
     8341 tgggtgtagt ggcggtaaaa gcggttccgc agcaaaatca gggggcggcg ctggcaactt
     8401 acaccgtatt tatggattta tcgcttggcg tgactggacc actggctggg ctggtgatga
     8461 gctgggcggg cgtaccggtg atttatctgg cggcggcggg actggtcgca atcgcgttat
     8521 tactgacgtg gcgattaaaa aaacggcctc cggaacacgt ccctgaggcc gcctcatcat
     8581 cttaaaactt actgaataac cagcgtatta atgatgtttt ctgcggtggt ctgcgctttt
     8641 tgctgatcgt cagcgggcag cgtaatttgc atggtcagca gttgattacc cacgttaccc
     8701 agaataacgg aagagtacgc cgtctggcct ttcgcggaga taatactgtc taactgctgc
     8761 attttgtgac ctttcagctc aatggcttta ttggttacca cttgcagctg cggatcgcgg
     8821 ctacgttgct gatcttccag acgcttcgcc agcaccgcca gatcttcttt cggatcatcg
     8881 cccatgatga cgatgactgc tttctgcccg gtggcgtcgg accagacatg catgttattg
     8941 gcctgcgttc ccagcttacc gctctggtcg gtcatatccg ctggcagcga gaaacttaac
     9001 ttgccatcaa gcaggttgac gggattcccg gtagcgttac tttccgcgac cgaaccctgc
     9061 gccgtagcgt tagtgtcttt atcatcacag gccgcaagcc ccataaccag caggccaatt
     9121 ccgacatatt taaccagatt gcgcattgac ttcttccttt cgataaacgg ccataacggc
     9181 tcattcatcc atcttatcac aactctgata acgaaccttt aactcgcctg caaagcgttg
     9241 atttcggatt tatctgccag tcttttcaac agcatattga gtaatacgcc atacattggc
     9301 aggaagaaaa cgatactgat taacactttg aaacagtaat cgaccagcgc gatttccatc
     9361 cagtgttcag ccataaaggc atccgggcta cgccagaagg caatgaagaa aaaggccagc
     9421 gtgtcgctga cgttaccgaa cagtgtggac gctgtcggtg ccagccacca gcggcgactc
     9481 tgacgcaggc ggttaaaaac gtgcacgtcg aggatttgcc ccagcgcgta ggccatgaaa
     9541 ctggcggtgg cgatacgggc gacaaacagg ttgaagtggg cgagtgcgcc gaatccctgc
     9601 caggaaccca tatagaatag cgacgagatg acgtaggaga ttaataacgc agggatcatt
     9661 accgcgaaga taatgcgtcg ggccagcggt gcgccaaaaa tacgcacggt caggtcggta
     9721 gcaagaaaaa taaacggaaa gctaaacgcg ccccaggtgg tatggaaacc caaaatggag
     9781 acgggaagct gaaccagata gttactggag gtgatcacca gcagatgaaa taacgataac
     9841 cagaacaacg ccttatagcg ttgagtttgc gagaaaacgt tcatattgta cctttttgat
     9901 taaccattgg ggtgagggaa cccaatacgt acgacacgtc tgttacctta acataataac
     9961 gtgcttaccc ttttttcgag ccgccgcatg atactgcttt acgttgacaa tgcaatggtt
    10021 aattttcacg caatcgttaa cctggtttgc ttacggactc acagggcgta aactagcgcc
    10081 gtttttttat gtgatgagaa gaaaatgacc gatctctttt ccagccctga ccacacactc
    10141 gacgcacttg gcctgcgctg cccggaaccc gtaatgatgg tgcgcaaaac tgtgcgcaat
    10201 atgcagcccg gcgaaacgtt gctgattatc gccgacgatc cggccactac ccgcgatatt
    10261 cctgggtttt gtacctttat ggaacacgaa ctggttgcga aagagacgga tggactgcct
    10321 tatcgttatt tgattcgtaa aggcggttga tgggggctga ttggcttcga tgccgccttt
    10381 tcccctcacc ctaaccctct ccccagaggg gcgaggggac cgatcgcgct caatgttgcg
    10441 atcggtttgc cttatctcct gcgcaacaat cttaacgcat tcgctgtcac cagcaccgtc
    10501 gcacccgtat ctgccagcac cgccagccat agtccggtca tccctaaaag cgtggtgacg
    10561 aggaagatcc ctttcagtcc cagcgcaatg gtgatgttct ggcggatatt ggcgtgagtg
    10621 gcgcgtgcca gttcaatcat ttgcaccagg ccgcgcaggt ggttatgggt taatgctgca
    10681 tcagcggttt ccagcgccac gtctgtgcca ctgcccattg caatcccaat ggcggcagct
    10741 ttcatcgctg gcgcgtcgtt aataccgtca ccgaccatcg ccagcggcgc atgttgattc
    10801 agctcggtca ccgctttgac tttatcttcc ggcaacaggc ccgctttaaa ctccagcccc
    10861 agctccccgg caattgccgc cgctgcgcgt ggattatcgc cggtgaggat cacccctttg
    10921 acgcccagcg cgttcagttc actgatggca gttgcagcat cggcgcgcag ggtatcctgt
    10981 aacgcaatga caccaagcac gtcatcgtta cgtactacca gcactaccgt ttgcccggcg
    11041 ctttccagtt cgttaattaa accagcaaat gcatcagcgg gatgtttccc ggcagcgcaa
    11101 atcaatacgc gctcaccgtt aacctgcgct tcaatgccag acccgaccag cgcccgctgt
    11161 gattcggcgg cgggaatggc gagtgcagca acctgtgctt cgcgcacgat cgcctgcgcc
    11221 agtggatgcg tcgcgccttg ctcgaccgcc gccgccagtg tcagcagttc agattcacta
    11281 atacccgttg ccggatgaat cgcggtaacg cgcggtttac cgacggtcag cgtaccggtt
    11341 ttatcaaacg ccacctgggt aacacgacct agctgctcca gcgccgcacc gcctttaatt
    11401 aacgccccac gacgtgccgc tgctgccagc ccggaggtaa tcgccgcggg cgtggagata
    11461 actaacgcac acgggcagcc aatcagcagc agcgtcagcc ctttataaat ccactcttgc
    11521 cagctggcgg caaacagcag cggcggcacc agcgttacca gcagggcgac ggccataatt
    11581 gctggcgtat aaatacggct gaaacggtcg ataaaccgct caatgggggc gcggcgctct
    11641 tcggcttctt cgatcagttt cagaatccgg tcaatggcgc tggcacccgg ttctgacagc
    11701 acttccagcg ttaccagacg gtctacgctg gtggctcctg caggaacttt atcgcccgtt
    11761 gcgcgctcca ccggaatgga ttcgccggtc agggcgcttt catcaaaact ggcaaagggc
    11821 gagagcagtt taccgtcggc aggtaaacgc ccgcccgccg caacttcaat cacatcgcca
    11881 gggcgcaggc tgttaatcgc cacctcttcc cgctcaccgt tacgcaggcg cgtggcggtt
    11941 tctggtttca gcgccattaa cgcgctaacg ccctgacgcg cgcggctggc ggcccagcct
    12001 tccagtcgtt caccaatcaa aaacagcagc aacaccatcg cagcttcagc cgttgcgcca
    12061 ataaacagcg caccaatagc agctacgctc attaacgttt caatggcgaa gtaactgcct
    12121 gatttgatca gccgtaatgc ctgacgagca atcgggtaca gcccaaccag cgtggtcgcg
    12181 ataaacgcca gttgcccgaa cggatgattg aactgctcca gaccccagct gattgccatc
    12241 atcacgatta gcgtaatcag cggcagattc tctttcaggc gcgatgcttg tggctcgtcg
    12301 gcggcctgct catcacgcag ggaatagccc gctttttgca ccgcagattc aacttgtgca
    12361 cggatgtcat tgtcggcatc aaccaccagt ttttcggtgg cgaacagtac ctgcacctga
    12421 ttcacgcctg caagctggcg cacggcattt tctaccttgc gcgcacaggc ggcgcagtcc
    12481 atgccgctga ctttccagct atagcgggtg ccggagacgt tttcagagag cgttggcgtg
    12541 ctggaacatg cgccgtcgca gcaacagtcg ttggcgttct gtaccgtggt tagcggtttg
    12601 aacgcggcaa attgaggggc tttcttgccg tgattgtcag gagtcgacat ggcatcctcc
    12661 ggttaagttt tttctcatta accgaaggat acactctgga gtcgactcca gagtcaagtt
    12721 ttatcagaga tacagcgagc ggacgatgag gaagtgaccg gcaaagtagc aggccgcagc
    12781 aatcgcgtta tccgcacgga agcgtcggcg atagtggcta cccagccaga caaagttact
    12841 gataaacagc aacgaagcgc cgacaaacgc agagagcgcc ggagcggtcg gacggaagaa
    12901 ccacagttca cctgccagcc acaccatcac cagcgtcatg ccgataaacg tgcagatagg
    12961 ccaacgatac tcttccagcc gcgtccagat aatcgccagt aacagcgcgc ccagaaccag
    13021 cagtaccaac ggcagaggcc agaagaaaga gagcgtcatc tgactggcga aatagatggt
    13081 gtacagcagg tgcgagagga aaaacgcgcc gatggcgtac atcagacgtt gacgtggcaa
    13141 cagggttagc gcatcgccca gcagtgaggc gcacagcccc gccagcacca gatagctaat
    13201 ggcgtcgaac atcggcgctt gccaggccag taacaacagg agaagaaggg ttaacggttt
    13261 aaacacccag cgttgccagg ttggcccacg atacgatgca tccacagata gccatgcgga
    13321 aagacagaca gcgataaacg accaaagcat cttagttcct tgaattcgtt attttttcgt
    13381 aagcggaatg cctacggtct tgcttccagt ttagtggcga gggtggacgg atgacaacac
    13441 cataactggc agggtatgct tatttccgca ttttccgatg agggataaag atgagcaaac
    13501 cacctctttt ctttattgtt atcattggct taattgtcgt cgccgcatcg tttcgtttta
    13561 tgcaacagcg acgggaaaaa gctgataatg atatggctcc gctccagcaa aagctggtgg
    13621 tggtgagcaa caagcgggaa aaaccgatta acgatcgccg ttcgcgccag caggaagtga
    13681 ctccggcagg caccagtatg cgctatgagg caagcttcaa accgcaaagc ggaggaatgg
    13741 agcaaacgtt tcgcctcgac gcccagcagt accacgccct gacagtgggc gataaaggta
    13801 cgctgagcta taaaggaacg cgctttgtta gctttgttgg cgaacaataa ggttattttt
    13861 taactttaaa tttcttctgc cagaccagca gttcaaacac gccgaaaagg aaaatccgca
    13921 ccttttcggc ggtggtcatc tgcgggccat ctttcggtaa agtggttttc aatagcgcca
    13981 gctgcatacc atgcatcagc acggtaaaaa tcagcgccac gttaacgaaa atattcagcg
    14041 ggcgcgggaa gggatgcacc aggttgagga ttaaaaatcc ccaaacgcag agcattaaca
    14101 aacgtccaat attaatcagc atcactttct ccttgtgctt cgcgttgata cagccgataa
    14161 gccacctgac ccgccacttt ttcccgatgc aatgaccagt ttgccggaac agtgggcaga
    14221 ccgttttcga cttcgctttc gacataaatc agggcttcgt cagccagcca gccgttatct
    14281 tccagtaaat ttatcgtctc ttctaacaag ccacggcgga acggtggatc gacaaacact
    14341 atattatgcg gtgtgccttt ttgcgccagg aatgacatcg cgttgctgtt cactacgcgt
    14401 gcattgcctg cttttagtgt cgccagattc ttaattaact gctgagaaac tgcacgatcc
    14461 atctcaatca acgtggcctc cgcagcgtag cgtgataacg cttccagccc cagcgcgccg
    14521 cttccggcga agcaatccag acattgggcg tcaacaatga ccggagccag ccagttaaat
    14581 aacgtttcgc gtacgcggtc ggtggtgggg cgcagacctg ggctatcagg aaccggtagt
    14641 ttacggcctc gccactgccc gccaataatg cggatttggc cgctgccgga atgattcggt
    14701 tttttcatga taaattgctc aatccgccag ataacacata cttgcaggcg gtgatgtgaa
    14761 ttaagttaag tgatagacta tttcatcatt tttttagctg ctatgtacat agcgttaacg
    14821 ctgtgccatg aagcaacagc gaggagtgta gtcgcaaatg gcgaaagaaa aaaaacgtgg
    14881 ctttttttcc tggctgggct ttggtcaaaa agagcagacc ccggaaaaag agacagaagt
    14941 tcagaatgaa caaccggttg tagaagaaat cgttcaggcg caagagcctg cgaaggcttc
    15001 tgaacaagcc gtcgaagagc aaccgcaggc gcatactgaa gccgaggcgg aaacttttgc
    15061 tgccgacgtt gtggaagtca ctgaacaggt tgctgaaagt gaaaaagcgc agcctgaagc
    15121 ggaagtcgtt gcacagccgg aaccggtcgt agaagaaacg ccggagccag tggctatcga
    15181 acgtgaagag ctgccgttgc cggaagacgt caacgccgaa gaggtttcgc cagaagagtg
    15241 gcaggctgaa gcggaaaccg tagagattgt cgaagcggcg gaagaagaag cggctaaaga
    15301 agaaatcacc gacgaagagc tggaagcaca ggcgctggct gccgaagcgg cagaagaggc
    15361 ggtgatggtg gttcctccgg tagaagagca gccggtggaa gaaatcgctc aggagcagga
    15421 aaaaccgacc aaagaaggtt tcttcgcgcg cctgaaacgc agcctgttaa aaaccaaaga
    15481 aaatctcggt tccggattta tcagcctgtt ccgcggtaaa aaaatcgacg atgatctgtt
    15541 tgaggagctg gaagaacagc tgttgatcgc cgatgtgggc gtggaaacca cacgtaaaat
    15601 tatcaccaat ctgacggaag gcgcatcccg caagcagctt cgtgatgccg aggcgctcta
    15661 tggcctgctg aaagaagaga tgggcgagat tctggcgaaa gtcgatgagc cgctgaatgt
    15721 tgaaggtaaa acgccgttcg tgatcctgat ggtgggcgtc aacggtgtgg gtaaaaccac
    15781 gacgattggt aagctggcgc gtcagtttga gcagcagggt aaatcggtga tgctggcggc
    15841 gggcgatact ttccgtgcag cagcggttga acagcttcag gtctggggtc agcgcaacaa
    15901 tattccggtg attgcccagc ataccggtgc ggattccgcc tctgttatct tcgacgccat
    15961 tcaggcagct aaagcgcgta atatcgacgt cctgattgcc gataccgccg gacgcctgca
    16021 gaacaaatcg catctgatgg aagagttgaa gaaaatcgtc cgcgtgatga agaaactcga
    16081 cgttgaagcg ccgcatgaag ttatgctgac tattgatgcc agtaccgggc agaacgcggt
    16141 aagccaggcc aaactgttcc acgaagccgt tggcttaacc ggcatcacgc taacgaaact
    16201 ggacggcacg gcgaaaggcg gggtaatttt ctcggtggct gaccagtttg gtatccctat
    16261 ccgctacatt ggtgtcggcg aacgtattga ggatttgcgt ccgtttaagg cggacgactt
    16321 tatagaggca ctttttgccc gagaggatta acaatgattc gctttgaaca tgtcagcaag
    16381 gcttatctcg gtgggaggca ggcgctgcag ggcgttacgt tccatatgca gccgggtgag
    16441 atggcgtttc tgaccggtca ttccggcgca gggaaaagta ccctcctgaa gctgatctgt
    16501 gggattgagc ggcccagcgc cgggaaaatc tggtttagcg gccatgacat cacgcgtctg
    16561 aaaaaccgtg aagttccgtt tctgcgccgc cagattggca tgattttcca ggatcaccat
    16621 ctactgatgg accgtactgt ctacgataac gtggcgatcc cgctgattat cgctggtgcc
    16681 agcggtgacg atattcgtcg ccgggtgtcg gcggcactgg ataaagtcgg gctactggac
    16741 aaagcgaaga acttccctat tcagctttcc ggcggtgaac aacagcgtgt tggcattgcc
    16801 cgcgcggtgg tgaataagcc cgcggtactg ctggcggacg aaccgaccgg taacctggac
    16861 gacgcgttgt cggaagggat tctacgtctg tttgaagagt ttaaccgcgt tggggtaacc
    16921 gtattgatgg caacgcacga catcaacctg atttcacggc gttcctatcg catgctcacc
    16981 ctgagcgatg gtcacttgca tggaggcgtg ggccatgaat aagcgcgatg caatcaatca
    17041 tattcggcag tttggtgggc gtctggatcg cttccgtaaa tcggtcggcg gctcaggcga
    17101 cggcggtcgt aacgcaccga aacgcgcgaa atcctcgcca aaaccggtaa atcgcaaaac
    17161 caacgttttc aacgaacagg tgcgctatgc cttccacggc gcattgcagg atctgaaaag
    17221 caaaccgttc gccacgtttt taacggtgat ggttatcgcc atctccctga cgctgcccag
    17281 cgtctgttat atggtgtaca aaaacgttaa tcaggcggcg acgcagtatt atccgtcacc
    17341 gcaaatcact gtttacttgc aaaaaacgct ggacgatgac gccgctgcgg gcgtggtggc
    17401 acagttgcag gccgagcaag gcgtggagaa agtgaactat ctttctcgtg aagacgcact
    17461 gggcgagttc cgtaactggt ctggttttgg tggtgcgctg gatatgctgg aagaaaaccc
    17521 gcttccggca gtggcggtgg tgatcccgaa actcgatttt caggggacgg aatcactcaa
    17581 tacgctgcgt gatcgtatca cgcagattaa cggcattgac gaagtgcgga tggatgacag
    17641 ctggtttgcc cgtctggcgg cgttgaccgg gctggtcggg cgcgtttcgg cgatgatcgg
    17701 cgtgttgatg gtggcggcag tgttcctcgt catcggtaac agtgtacgcc tgagtatctt
    17761 tgctcgtcgt gactccatta acgtgcagaa actgattggt gcaacggatg gtttcatcct
    17821 gcgcccgttc ctgtacggtg gcgcattgct ggggttttct ggcgcattgt tgtcactaat
    17881 tttgtcagaa attctggtac tgcgattgtc atcggcggtt gcggaagtgg cacaggtttt
    17941 cggaacgaag tttgatatca atggcttatc gttcgatgaa tgcctgctat tgctgctggt
    18001 atgctcgatg attggctggg tggcagcgtg gcttgccacg gtacaacatt tacgccactt
    18061 tacgcctgaa taataaaagc gtgttatact ctttccctgc aatgggttcc gtagcaggga
    18121 aagagacccc gttgtctctt cccggtattt catctctatg tcacattttg tgcgtaattt
    18181 attcacaagc ttgcattgaa cttgtggata aaatcacggt ctgataaaac agtgaatgat
    18241 aacctcgttg ctcttaagct ctggcacagt tgttgctacc actgaagcgc cagaagatat
    18301 cgattgagag gatttgaatg actgacaaaa tgcaaagttt agctttagcc ccagttggca
    18361 acctggattc ctacatccgg gcagctaacg cgtggccgat gttgtcggct gacgaggagc
    18421 gggcgctggc tgaaaagctg cattaccatg gcgatctgga agcagctaaa acgctgatcc
    18481 tgtctcacct gcggtttgtt gttcatattg ctcgtaatta tgcgggctat ggcctgccac
    18541 aggcggattt gattcaggaa ggtaacatcg gcctgatgaa agcagtgcgc cgttttaacc
    18601 cggaagtggg tgtgcgcctg gtctccttcg ccgttcactg gatcaaagca gagatccacg
    18661 aatacgtcct gcgtaactgg cgtattgtca aagttgcgac caccaaagcg cagcgcaaac
    18721 tgttcttcaa cctgcgtaaa accaagcagc gtctgggctg gtttaaccag gatgaagtcg
    18781 aaatggtggc ccgtgaactg ggcgtaacca gcaaagacgt tcgtgagatg gaatcacgta
    18841 tggcggcaca ggacatgacc tttgacctgt cttccgacga cgattccgac agccagccga
    18901 tggcaccggt gctctatctg caggataaat catctaactt tgccgacggc atcgaagatg
    18961 ataactggga agagcaggcg gcaaaccgtc tgaccgacgc gatgcaaggt ctggacgagc
    19021 gtagccagga catcatccgc gcgcgctggc tggacgaaga caacaagtcc acgttgcagg
    19081 aactggctga ccgttacggc gtttccgctg agcgtgtgcg ccagctggaa aagaacgcga
    19141 tgaaaaaatt gcgtgctgct atagaagcgt aatttccgct attaagcaga gaaccctgga
    19201 tgcgagtccg gggtttttgt tttttgggcc tctgtaataa tcaatttccc ctccggcaaa
    19261 acgccaatcc ccacgcagat tgttaataaa ctgtcaaaat agctattcca atatcataaa
    19321 aatcgggtat gttttagcag agtatgctgc taaagcacgg gtagtcatgc ataaaacgaa
    19381 ataaagtgct gaacaacaac atcacaacac acgtaataac cagaagaatg gggattctca
    19441 ggatgaacat aaagggtaaa gcgttactgg caggatgtat cgcgctggca ttcagcaata
    19501 tggctctggc agaagatatt aaagtcgcgg tcgtgggcgc aatgtccggt ccggtggccc
    19561 agtacggtga ccaggagttt accggcgcag agcaggcggt tgcggatatc aacgctaaag
    19621 gcggcattaa aggcaacaaa ctgcaaatcg taaaatatga cgatgcctgt gacccgaaac
    19681 aggcggttgc ggtggcgaac aaagtcgtta acgacggcat taaatacgtg attggtcacc
    19741 tctgttcttc atcaacgcag cctgcgtctg atatctacga agacgaaggc attttaatga
    19801 tcaccccagc ggcaaccgcg ccggagctga ccgcccgtag ctatcagctg atcctgcgca
    19861 ccaccggcct ggactccgac caggggccga cggcggcgaa atatattctt gagaaagtga
    19921 aaccgcagcg tattgctatc gttcacgaca aacagcaata cggcgaaggt ctggcgcgag
    19981 cggtgcagga cggcctgaag aaaggcaatg ctaacgtggt gttctttgat ggtatcaccg
    20041 ccggggaaaa agatttctca acgctggtgg cgcgtctgaa aaaagagaat atcgacttcg
    20101 tttactacgg cggttatcac ccggaaatgg ggcaaatcct gcgtcaggca cgcgcggcag
    20161 ggctgaaaac tcagtttatg gggccggaag gtgtggctaa cgtttcgctg tctaacattg
    20221 cgggcgaatc agcggaaggg ctgctggtga ccaagccgaa gaactacgat caggttccgg
    20281 cgaacaaacc cattgttgac gccatcaaag cgaaaaaaca ggacccaagc ggcgcattcg
    20341 tttggaccac ctacgccgcg ctgcaatctt tgcaggcggg cctgaatcag tctgacgatc
    20401 cggctgagat cgccaaatac ctgaaagcga actccgtgga taccgtgatg ggtccgctga
    20461 cgtgggatga gaaaggtgat ctgaaaggct ttgagttcgg cgtatttgac tggcacgcca
    20521 acggcacggc gaccgatgcg aagtaatcat taatcggcaa ctttgggttg ccgccaaatt
    20581 gctaatatcg agtacgttgc ttcatgccgg atgcggcgta aacgccttat ccggcctaca
    20641 agatccaaag aaatcagtaa attgcaacac acattgtagg cctgataagc gtagcgcatc
    20701 aggcaatttc tcgttggtca tcacttttga aatcaacttg acgattaacg cttctcccag
    20761 ccgccctgtt gcgccgtaaa tcccagtgcc tgcataaacg ccgtcatcac accgcgatct
    20821 tccacgcctg catccgccat ccaccagcat gaaacgccag gattgttacg caaaacctct
    20881 tccagcagat attgccccac accgcgacgt cgggtgactt cccgcacgcg cagggaatcc
    20941 agtgccccct cggtgccgct taaggttacc cgcacggccg caagcagacg ctcgttaaaa
    21001 cgcgcggcgt agatacggtg gttatcgtca acctgtaaag aggaagggga atactccggc
    21061 cagatctttt gcaggtcgat ccggtcctgg tcgctaaagt tttctaatcg aatgatggtc
    21121 agcttcatgg gtaacccgtg taaatcacaa aagtggaacc agtgtagcga aataatttaa
    21181 tcggaggctt tctctttttt atttcttttg gcaggtgatt aattttttaa cagcaataat
    21241 tacaaagtta aaac
//
LOCUS       DAOFQD010000077        20970 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.077, whole genome shotgun sequence.
ACCESSION   DAOFQD010000077 DAOFQD010000000
VERSION     DAOFQD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.077"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            115..267
                     /gene="hokA"
                     /locus_tag="QBF46_002992"
     CDS             115..267
                     /gene="hokA"
                     /locus_tag="QBF46_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDQ6675149.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(452..664)
                     /gene="cspA"
                     /locus_tag="QBF46_002993"
     CDS             complement(452..664)
                     /gene="cspA"
                     /locus_tag="QBF46_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDQ6675150.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(945..1235)
                     /locus_tag="QBF46_002994"
     CDS             complement(945..1235)
                     /locus_tag="QBF46_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDQ6675151.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1669..2379
                     /locus_tag="QBF46_002995"
     CDS             1669..2379
                     /locus_tag="QBF46_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDQ6675152.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQMNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2429..3403)
                     /gene="ghrB"
                     /locus_tag="QBF46_002996"
     CDS             complement(2429..3403)
                     /gene="ghrB"
                     /locus_tag="QBF46_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDQ6675153.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3507..4166)
                     /locus_tag="QBF46_002997"
     CDS             complement(3507..4166)
                     /locus_tag="QBF46_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDQ6675154.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4319..6652
                     /gene="bisC"
                     /locus_tag="QBF46_002998"
     CDS             4319..6652
                     /gene="bisC"
                     /locus_tag="QBF46_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDQ6675155.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNSEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQERGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6621..7061)
                     /locus_tag="QBF46_002999"
     CDS             complement(6621..7061)
                     /locus_tag="QBF46_002999"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ6675156.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7058..7621)
                     /gene="tag"
                     /locus_tag="QBF46_003000"
     CDS             complement(7058..7621)
                     /gene="tag"
                     /locus_tag="QBF46_003000"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDQ6675157.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFVDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7779..8477
                     /locus_tag="QBF46_003001"
     CDS             7779..8477
                     /locus_tag="QBF46_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6675158.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8706..9914
                     /locus_tag="QBF46_003002"
     CDS             8706..9914
                     /locus_tag="QBF46_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6675159.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALLMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10238..11929
                     /gene="eptB"
                     /locus_tag="QBF46_003003"
     CDS             10238..11929
                     /gene="eptB"
                     /locus_tag="QBF46_003003"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDQ6675160.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            12021..12097
                     /locus_tag="QBF46_003004"
     tRNA            12021..12097
                     /locus_tag="QBF46_003004"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12055..12057,aa:Pro,seq:cgg)
     gene            12842..14449
                     /gene="dppA"
                     /locus_tag="QBF46_003005"
     CDS             12842..14449
                     /gene="dppA"
                     /locus_tag="QBF46_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDQ6675161.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVDLYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14600..15619
                     /gene="dppB"
                     /locus_tag="QBF46_003006"
     CDS             14600..15619
                     /gene="dppB"
                     /locus_tag="QBF46_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDQ6675162.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15629..16531
                     /gene="dppC"
                     /locus_tag="QBF46_003007"
     CDS             15629..16531
                     /gene="dppC"
                     /locus_tag="QBF46_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDQ6675163.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16542..17525
                     /gene="dppD"
                     /locus_tag="QBF46_003008"
     CDS             16542..17525
                     /gene="dppD"
                     /locus_tag="QBF46_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDQ6675164.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17522..18526
                     /gene="dppF"
                     /locus_tag="QBF46_003009"
     CDS             17522..18526
                     /gene="dppF"
                     /locus_tag="QBF46_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDQ6675165.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18556..19827)
                     /locus_tag="QBF46_003010"
     CDS             complement(18556..19827)
                     /locus_tag="QBF46_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDQ6675166.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20303..20410
                     /locus_tag="QBF46_003011"
     CDS             20303..20410
                     /locus_tag="QBF46_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDQ6675167.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20786..20893
                     /locus_tag="QBF46_003012"
     CDS             20786..20893
                     /locus_tag="QBF46_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDQ6675168.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         5005 a         5208 c         5547 g         5210 t
ORIGIN      
        1 gcctcgtgtt taactgtcca actttttggg gtcagtacaa agtgaggcta ccctatgcct
       61 gaacaccata aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg
      121 cagaaatata gattactttc tttaatagtg atttgtttca cgcttttatt tttcacctgg
      181 atgataagag attcactgtg tgaattgcat attaaacagg agagttatga gctggcggcg
      241 tttttagcct gcaaattgaa agagtaagag tcttcggcgg gaaattattc ccgccttact
      301 tacggcgttg cgcattctca ttgcacccac atttattctt cacaaaaata ataatagatt
      361 ttattacgcg atcgattatt tatttcctga aaaacaaaaa aaatccccgc caaatggcag
      421 ggatcttaga ttctgtgctt ttaagcagag attacaggct ggttacgtta ccagctgccg
      481 ggcctttagc gccgctttcg atggtgaagg acactttctg accttcgtcc agagatttgt
      541 aaccatcgtt ctggatagca gagaagtgta cgaacacatc tttagagcca tcgtcaggag
      601 tgatgaagcc gaagcctttg tcagcgttga accattttac gataccagtc attttaccgg
      661 acatagtgta ttacctttaa taattaagtg tgcctttcgg cgatatggcg tgctttacag
      721 attttgaagc gttaaaggaa tgtgcactac gaggggtatc aacgataact cttgaaggga
      781 cttgccttac tacactgctt taatggtctg tacgtcaaac cgttgatgtg cattaagcca
      841 cgcattggcg ggtgatgcaa caattatttt tcatatttat gattaatcgg ttggaaaacg
      901 gtcctgtcat caggaccgta aacagcaata aagtggataa aagtctattc catcaactgc
      961 ttacttaatg ccgggttggc ttgaatcaaa cgcatcaatt ttagttcggc acttgaaggc
     1021 ttcacgcgtc tggattccca ttcctttacc atggcgactg atacgcccaa aacccgggcg
     1081 aaatcatcga tttttaaccc tgtccctttt cgtaactgct caatttcggt acaggacgtt
     1141 gttctgtgcg tcagggtaat tttctgcgtt tcatctttaa aaacaatctg ttccaggctg
     1201 ctcaacagct catgcattgg atctttatat tccattgaaa actcctcaaa tcacactgcg
     1261 ggatcgtgaa ttgcatcgaa gctcattaag aatagtcggg aaaagcagac acagcggggt
     1321 gaagtcaggg catgctctgg ctatgattcg attcagcctg tcatttagcg tgacttatag
     1381 ccacctaacg atctgataaa tgccgatata tctcttacca ctcctgtttt gtaaagattt
     1441 cgaaaatcta accttctgcg aaggcgtaaa tctctggcta caaaaaaggc aacggcacta
     1501 tcatttttta acaatgaaac aacaaattaa ccttgtttca ttttctccat gattttacca
     1561 ggaagattac gtagaaaggg tatcctgcaa gcctgtcctg gactatcctt gtcacgtcag
     1621 acacgcgtgt gtcgttgtgc gctttttttg ggtgaaagga gtaagaaaat ggcgacagga
     1681 aagtcctgct ctcgctggtt tgcgcctctt gcggcgttat taatggtagt tagcctgagt
     1741 gggtgttttg ataaagaagg cgatcagcgt aaagcgttta tcgacttcct gcagaataca
     1801 gtgatgcgta gcggtgaacg tctaccaacc ctgactgccg atcagaaaaa acagtttggt
     1861 ccttttgtct ctgattacgc gattctgtat ggttattctc agcagatgaa tcaggcgatg
     1921 gattccggtc tgcgcccggt tgtagacagc gttaacgcaa ttcgcgtacc gcaggattac
     1981 gttacgcaaa gcggtccgct gcgtgaaatg aacggttcgc tgggtgtgct ggcgcaacaa
     2041 ctgcaaaatg cgaagttgca ggccgatgct gcgcactctg cgctaaaaca gagcgatgat
     2101 ttgaaaccgg tctttgatca ggcattcacc aaagtggtaa cgacgccagc tgatgcattg
     2161 caaccgttga ttccggcggc acaaactttc acgcaacaac tggtgatggt tggagactac
     2221 attgctcagc aaggtactca ggtgagcttt gtcgcaaatg gcatccagtt cccgacttca
     2281 cagcaggcaa gtgaatataa caaactgatt gcgccattac cggcacagca tcaggcgttt
     2341 aatcaggcct ggactacagc agttactgca acccaataaa gagtaaaagc ccgagcgatt
     2401 atcgggcttt actctacgca gtcgcggctt agtccgcgac gtgcggattc acacagttct
     2461 tctcaacctt tccttgtaac gcatcaatca aattatccac ggcacaggcg gccatgccat
     2521 aacgcgtctc atgggtggca gatccaatat gcggtactgc gacgacgttg gccattgaga
     2581 gcaacggcga atctacggac agtggctctt gttcgaagac atccagcccg gcagcgtgaa
     2641 tttcgccttt ctgcaatgct gcgatcagtg cattttcgtc aaccaccggg ccacgtccgg
     2701 cattaatgaa aatggcggag gatttcattt tggcgaattg ttctgcgcca aacagatgat
     2761 gcgtctcatc agttaacggc aggatcaggc aaacgaaatc tgactcttgt aacagagtat
     2821 ccaaatcgca gtagcgggcg ttgaagcgtt cttctgcttc tttatggtgg cggcgcgcgt
     2881 tatagaggat gggcatgttg aagccaaagt gcgcacgttg tgccagcgcc atgccgatcc
     2941 gtcccatccc gacaatgccc agtgttttat ggtgaacgtc agtgccgtac cagtccgggc
     3001 ctatgctcgc ggtccattcg cctgctttta cccgttctgc tacctccaca acccgacgag
     3061 cggtagacaa caccagcgcc atcagcgtat cggcgacggt ttctgttaat acggttggcg
     3121 tgtgcatcag cagaattttt cgggcggtaa gcgcatcgac atcaaaattg tcatagccga
     3181 cggagatcgt tgatgtggca cgcagtttcg gcattttttc cagcaatgcg gcatcaacat
     3241 tctcgtttga acccagtaaa ccttcagctt cggcaaaaat tgctgcattt tgttcgacgg
     3301 tttgtgggct gaggtttgcc acctggtgaa cggtgaaatg ctcttgcagg cgttgcagta
     3361 aatcatcagg taaggctttg tagaggataa cggacggctt catgcttctc tccattcact
     3421 gataaggcga tcaccttagc ctgaaatatg atgaaggaaa actgaagaat gccaggtgat
     3481 ttctgcatca cctggcatga aagggattac agcgggctta aggtaatttc tacacggcgg
     3541 ttttgcgcct taccttctgc ggtgctgttg ctggcgattg ggttagccgg gccaaggccc
     3601 tgagtacgga tgcggctggc gtccacgccc tgggtgatca acgcgctggc aacggaatcc
     3661 gcacgctgct gggagagacg catgttcagg tcgtgacccc ccgtgctgtc ggtataacca
     3721 atcacgttaa ccgccgtttt cggatactct ttcagcacca ttgccacgcc ggtcagggtg
     3781 ttagcgcctg ccggtttcag ggtcgcgctg ctgctgtcga aggtcacatt gttcggcata
     3841 ttgaggataa tgttatcccc gctgcgggtt acgctaacac cagtgccgcg cattttgtca
     3901 cgcagcttcg cttcctgcac atccatgtaa taacccacgc cgccgcccag agctgcgcct
     3961 gctgctgcgc caatcagcgc gcctttaccg cgatctttct tcgaagaaga gagcgcacca
     4021 atacccgcgc ccacgagaga gcccagacct gcgccgatag cagatttgcc tgcttcgcgt
     4081 tcgccggtgt aagggttagt tgtgcagcca gataccgcca gagcgccact cactacggcg
     4141 gcaataagat aaacacgttt cttcattgtt aatccttaat aaccttttta ttctttgcca
     4201 cgggttccgt ggcgggagat tatgccgcgt gaacatgaag attattcctg ggaatactcg
     4261 gaaatttgta agtaatattt aactgctcaa tacatctaac ctttcaggag tcttcggttt
     4321 ggccaactca tcctcacgat attccgttct gactgccgcc cactgggggc ccatgctggt
     4381 tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg ttagccacag gaatggaaaa
     4441 ctccttgcag agcgcggttc gcgaccaggt tcacagcaat acgcgggtac gatttccaat
     4501 ggtgcgcaaa ggctttctcg cgtcaccgga aaacccgcaa ggcattcgtg ggcaggatga
     4561 atttgttcgc gtgagttggg atgaggcgct ggatcttatt caccaacaac ataaacgcat
     4621 tcgtgaggct tatggtccgg catcgatttt tgctggttcc tacggctggc gttcaaacgg
     4681 cgtgctgcat aaggcctcga cattattaca acgctatatg gcgctggcag gcggttatac
     4741 cgggcatctg ggggattatt cgaccggcgc ggcacaggcg atcatgccgt atgtcgtggg
     4801 tggtagcgaa gtttatcaac agcagaccag ttggccgctg gtgctggaac atagcgatgt
     4861 cgtggtgctg tggagtgcta atccactcaa tacgctgaaa attgcgtgga atgcatccga
     4921 tgagcagggg ctttcttact tttccgcact gcgtgacagc gggaaaaagc tgatctgcat
     4981 tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat aaaatggagt gggtggcacc
     5041 gcacatgggc accgatgttg cgctgatgct ggggatcgcc catacgctgg tggaaaatgg
     5101 ttggcacgac gaagcgtttc tggcgcgttg caccacaggt tatgccgtct tcgcctctta
     5161 tttgctgggc gagagtgacg gaatagcgaa aaactccgaa tgggcagcag agatttgtgg
     5221 tgttggcgca gcgaaaatcc gcgagctggc ggctattttc caccaaaata ccaccatgct
     5281 gatggcaggc tggggaatgc agcgccaaca gtttggtgag caaaaacact ggatgatcgt
     5341 cacgctggca gcaatgttgg ggcaaatcgg cacacccggc ggcggttttg gtctttctta
     5401 ccattttgcc aatggtggta accccacgcg gcgttctgcg gtgctctctt ccatgcaggg
     5461 cagcttgccg ggtggctgcg atgcggtgga taaaatccct gttgcccgca ttgttgaagc
     5521 actggaaaac cctggtggcg catatcaaca caacggtatg aaccgacatt tcccggatat
     5581 tcgttttatc tggtgggcgg gcggtgccaa ctttactcat catcaggata ccaatcgtct
     5641 gatccgtgcc tggcaaaaac cggagctggt ggtgatctct gaatgcttct ggacggcggc
     5701 ggcgaaacac gcggatatcg ttctgcctgc gactacctct tttgagcgta atgatctcac
     5761 catgaccggt gattacagta atcagcatct ggtgccgatg aagcaagtgg tgccgccacg
     5821 ctatgaagcg cgtaatgatt ttgatgtttt tgccgagttg agtgaacgtt gggagaaggg
     5881 cggctatgcg cggtttacgg aaggaaaaag tcagctgcaa tggctggaaa cgttttataa
     5941 cgttgcccgg cagcgcgggg caagccagca ggttgaattg ccgccatttg ctgagttctg
     6001 gcaagccaac cagttaattg agatgccgga aaacccggac agcgagcggt ttattcgctt
     6061 cgctgatttt tgccgcgatc cgctggcgca tccgttaaaa accgccagcg gcaagattga
     6121 aatcttctca cagcgtattg ccgattacgg ttacccggat tgccctgggc atccaatgtg
     6181 gctggagccg gacgaatggc agggcaatgc cgaaccagaa cagttgcagg tactttctgc
     6241 ccatccggcg caccgcctgc acagccagct gaattacagt tctctgcgcg aattgtacgc
     6301 ggtggcaaat cgtgagcctg tcaccattca tcctgacgat gcccaggagc gcggcataca
     6361 agatggcgat actgttcggt tgtggaacgc acgcgggcaa attcttgccg gagcggtcat
     6421 tagcgaggga attaaacctg gcgtgatttg cattcacgaa ggggcatggc cggatctgga
     6481 tttaaccgct gacggtattt gtaaaaacgg cgcagtgaac gtgctgacca aagatctccc
     6541 cagctcgcgg ctggggaatg gctgtgcggg taatacggcg ctggcatggc tggaaaaata
     6601 caacggtccg gaactgacac ttacagcgtt tgaaccaccg gccagctcat aatccatgtg
     6661 ggtagttggg tttcatcctg ccatgcgcaa tcgacaatgt gaaaaccctg tgcctggtaa
     6721 aaatttatcg ccggttgatt tttttgataa acctccagca tcaggtgggg atggcgctgc
     6781 tgcacatact gcatcagcgc cttaccaata ccgcgcctga cggccttcgg tgcgacaaac
     6841 atcgctgcca gaaatcggcc ttccataatg ctgacaaaac cgagaagctt accgtcttct
     6901 tcccagaccc agttttgcgc gttggcaaga taggcatccc gcaccagcgg aatgcagtca
     6961 cgccagtaat tcgcttttat aaagggatgc ccccaggttg tactttccag ccacagttcg
     7021 aggatcgcgg ggagttctga acgttgcgct tcccgaatca tggtttattt cccggatagc
     7081 aacagcagcc aaccacatga tcattcacca gcccacatgc ctgcataaag gagtaacaga
     7141 ttgtggtgcc gacaaactta aaaccacgtt ttttcagtgc cttagatagg gcgtcggagg
     7201 cggacgtaga tgtgggaatt tcgctcaacg ttgtggcttg tgtcacctgt ggctgatgat
     7261 ttacaaacga ccagacaaag tcgacaaacg gttcgccgtt ctgttccatt tgcaggtacg
     7321 cccgcgcatt accaataatt gcctgaattt tccctcgatg gcggataatc ccggcgtcct
     7381 gtaccagtct ttcgacatcc tcttcctgca ttgctgcgac cttcaccgga tcgaactgat
     7441 gaaagcaggc gcgatagttt tcgcgttttt tgaggacggt gatccacgat aacccagcct
     7501 gctgcccttc aaggcagatc atttcgaaca gttttttact gtcagtttca ggcacgcccc
     7561 actcattatc atggtaggca atataaagcg ggtcctgact cacccagccg caacgttcca
     7621 tactttccct cgctatgtac ccgatcgtaa aaatttcagc cgcttttctt gacgcatcgg
     7681 tgaaaaagtc aatatttaat acagggctac tatgcccgat atctttcata caatgacaat
     7741 aatattgccg aattcgactc ttccctggag tcgtgttaat gatcataaaa aaaagcggtg
     7801 gtcgctggca gctaagcctg ctggcgagcg tggtaatcag tgcctttttt ctcaacacag
     7861 cttacgcctg gcaacaagaa tatatcgttg atacgcaacc cggacattcc acagagcgtt
     7921 acacctggga tagtgatcat caacctgatt acaacgatat tttgtcgcaa cgtattcaaa
     7981 gtagccaaag ggcgctggga ctggaagtca atctggcaga agaaacccct gtggatgtga
     8041 ccagcagtat gagtatgggc tggaattttc ctttatatga acaggttaca accggcccgg
     8101 tcgcggcatt acattacgat ggcacaacca cctcgatgta taacgagttt ggcgacagta
     8161 ctaccacgct gaccgatccg ttatggcatg ccagcgtgag taccttaggc tggcgtgttg
     8221 actcccggct tggcgatctc cgaccctggg cgcaaatcag ctataaccag caatttggcg
     8281 agaatatctg gaaggcgcaa tcaggcctga gccggatgac ggcgacaaac cagaacggca
     8341 actggctgga tgtcaccgta ggcgctgata tgttgctcaa tcaaaatatt gccgcctatg
     8401 ccgcgctaac tcaggcagaa aataccacta ataatagcga ctatctgtat acgatggggg
     8461 ttagcgccag attttaacgt aacagtcaca attgaaacca ttaaataaca atagttgtgg
     8521 cgatagtggg tgctaactta ccaaataata aatttggtga ataattgtcg cgtcattcat
     8581 tcctgaacta aggcatttca ttccgttctg atggcatttc atgccgtttt tccccaggca
     8641 taaagtgcac ttcgttatgg ttgtcggcag agatttttcc tttttattac tgcaggaata
     8701 ctgccatgac accttcaaat tatcagcgta cccgctggct gacactcatc ggtactatca
     8761 ttacccagtt tgcgctgggg tcggtttata cctggagcct gtttaatggc gcgctttccg
     8821 ccaagctgga tgcgccggta agccaggtcg ctttctcttt cggcttgtta agtctggggc
     8881 tggcaatttc gtcttctgtt gcgggcaaat tacaggaacg ttttggcgtt aaacgcgtca
     8941 ccatggcttc cggcattttg ctgggattag gcttcttcct gacagcgcat tctgacaacc
     9001 tgatgatgct gtggttaagc gccggtgtgc tggtgggact ggcagatggc gcgggttatc
     9061 tgctgacgct ctctaactgt gtgaagtggt tcccggagcg taaaggtctg atctccgcgt
     9121 tcgctatcgg ttcttatggt ctgggtagcc tgggtttcaa atttatcgac acgcagctgc
     9181 tggaaacggt cggtctggaa aaaacctttg tgatttgggg agcgattgcg ctgttgatga
     9241 ttgttttcgg cgcaacgtta atgaaagacg caccaaaaca ggaagtgaaa accagcaatg
     9301 gtgtggtgga gaaagattac acgctggcag agtcgatgcg taaaccgcag tactggatgt
     9361 tagcggtaat gttcctgacc gcctgcatga gcggcctgta cgtgattggg gtagcgaaag
     9421 atatcgccca aagtctggca caccttgatg tggtttccgc agccaatgca gtcactgtta
     9481 tttccatcgc caacctttca ggtcgtctgg tgctgggtat tctgtctgac aaaatcgccc
     9541 gtatccgtgt tattaccatt ggtcaggtga tatcgctggt gggtatggcg gccctgctgt
     9601 ttgcaccatt gaatgcagtg acgttctttg cagcgattgc ctgcgtggca tttaactttg
     9661 gcggcactat taccgtcttt ccgtcactgg tcagtgagtt ctttggcctc aataacctgg
     9721 cgaaaaacta cggtgtgatt tatctcggtt tcggtatcgg tagcatttgt ggttcgatta
     9781 tcgcctcact gtttggcggc ttctatgtga ccttctacgt gattttcgcc ctgctgattc
     9841 tgtcattggc gctttctacg acgattcgtc agccagagca gaaaatgttg cgtgaggcgc
     9901 atggctccct ttaatgctca gccgcgagct actgggcttc agcttccgtg tgcatcaaac
     9961 gcatcaacga aatgtataaa caatgactcg taaaatttgg gccaattaac tggccctttt
    10021 tactgctttt tgtaacgcta aagtctcttt tcaaacttgc atttttgtaa atttgtgctt
    10081 catgcacact ctttccccac actttttccc tttgctgtgg tctacttatt cgcgcgtgta
    10141 gattttactt atctgactac ctccgcactt tttccctgcc gggcctgaaa agccactaag
    10201 cagggtgtta tcacctgttt gtccagggtt tgtttgcatg agatacatca aatcgattac
    10261 acagcagaag ctgagctttt tgcttgcaat ctatattggc ctttttatga atggcgcggt
    10321 tttttaccgc cgcttcggca gctatgcgca cgattttacc gtctggaaag gcatttctgc
    10381 tgttgttgaa ctggccgcca ccgtactggt gaccttcttt ttactacgtc ttctttcgct
    10441 gtttggccgc cgcagctggc gtattctggc atcgctggtg gtgctctttt ccgcaggtgc
    10501 cagctattac atgaccttcc ttaatgtggt cattggttat ggcatcatcg cttccgtcat
    10561 gaccaccgat atcgacctgt caaaagaagt tgttggtctg aactttattc tctggttaat
    10621 cgccgttagt gcattgcctc ttatccttat ctggaataac cgctgtcgct acaccttgct
    10681 ccgacaactg cgaaccccag ggcagcgtat tcgcagcctg gcggtcgtcg tactggcggg
    10741 tattatggtt tgggcaccga ttcgtttgct ggatatccag cagaagaaag tggagagggc
    10801 gaccggcgtt gatttgccga gttatggcgg tgtcgtagcg aactcttatc tgccatcaaa
    10861 ctggctttct gcgttggggc tgtatgcctg ggcgcgggtc gatgaatctt ccgataataa
    10921 ttcattgctt aatccggcga agaaattcac ttatcaggca ccgcaaaacg ttgatgacac
    10981 ttatgtcgtg tttatcatcg gtgaaaccac gcgttgggac catatgggta ttttcggcta
    11041 tgagcgtaat accacgccga aactggccca ggagaaaaat ctggcggcgt tccgtggtta
    11101 ctcctgtgat accgcaacca aactctcact gcgttgcatg tttgtacgtc aggggggcgc
    11161 ggaagataat ccgcagcgca cattaaaaga acagaacatt ttcgcggttc tgaagcagtt
    11221 aggattcagt tctgacctct acgctatgca gagcgaaatg tggttctaca gcaacacgat
    11281 ggcggacaac attgcttatc gtgagcagat tggtgcggag ccacgtaatc gtggcaagcc
    11341 ggtagatgat atgttgctgg tagacgaaat gcagcaatcg ctagggcgca acccggatgg
    11401 taagcatctg atcattctgc ataccaaagg ttcgcatttt aactacaccc agcgttatcc
    11461 gcgtagcttc gcgcagtgga agccggaatg tattggtgtt gatagcggct gtaccaaagc
    11521 gcagatgatc aactcctatg acaactcggt gacctatgtg gatcacttta tctccagcgt
    11581 gattgatcag gttcgcgata agaaagcgat tgtgttctac gcagctgacc acggtgagtc
    11641 aattaatgaa cgcgagcacc tgcacggcac gccgcgtgaa ctggcaccgc cggagcagtt
    11701 ccgcgtaccg atgatggtct ggatgtcaga taaatatctg gaaaatccgg ccaatgcgca
    11761 ggcgtttgcg cagctgaaaa aagaagccga catgaaagtg ccacgccgtc acgtagagct
    11821 gtacgatacc atcatgggtt gtcttggcta tacttcaccg gatggtggaa ttaacgaaaa
    11881 caacaactgg tgtcacatcc cgcaggcaaa agaggcagcg gctaactaag cggcctgctg
    11941 actttctcgc cgatcaaaag gcattttgct attaagggat tgacgagggc gtatctgcgc
    12001 agtaagatgc gccccgcatt cggtgattgg cgcagcctgg tagcgcactt cgttcgggac
    12061 gaaggggtcg gaggttcgaa tcctctatca ccgaccaaat tcgaaaagcc tgctcaacga
    12121 gcaggctttt ttgcatctgc agctcatgag gatgagaacc tccgggggca gggaggttcg
    12181 actcgacgta ggcctgataa gacgcgtcag cgtcgcatca ggcaacggca ccgcacttat
    12241 tgtcggatgc ggcgcgaccg ccttattcga cctacacacc acacatttcc ctgccaaatt
    12301 tgcgccccat atctcactat ccattaacgg atttgtgaca gaaaccattg tcttttttta
    12361 tatttgctta aatctttttt tgcgttactt atggctggcg ctggcatttt ccgctgtgcg
    12421 ttttagcgtt catggtatta atcgctcaga tatccacctt tttctcagat ttttttcact
    12481 aaatgcataa aacttaatca ccaaatgtta ctaaatatta agctgattgt tctggtcatg
    12541 ttggagtagc acaaatttct ctgctgcaaa tagctgtttg aagttttttt aatctttgtt
    12601 tgtggattct caccgttggt gtaaatcccg gttggctgta ttgacgtttt cacattctgt
    12661 tgacagattg taggtcacga ggggcatttt atggaggatc cgcactgtta cactgatgtt
    12721 aattagtacg gcatccccac ctcataacgt tgacccgacc gggcaaaaaa caaaaaaggt
    12781 caggcagcga caacccactg caaagggtta aaacaacaaa catcacaatt ggagcagaat
    12841 aatgcgtatt tccttgaaaa agtcagggat gctgaagctt ggtctcagcc tggtggctat
    12901 gaccgtcgca gcaagtgttc aggctaaaac tctggtttat tgctcagaag gatctccgga
    12961 agggtttaac ccgcagctgt ttacctccgg caccacctat gacgcctctt ccgtcccgct
    13021 ttataaccgt ctggttgaat ttaaaatcgg caccaccgaa gtgatcccgg gcctcgctga
    13081 aaagtgggaa gtcagcgaag acggtaaaac ctataccttc catctgcgta aaggtgtgaa
    13141 gtggcacgac aataaagaat tcaaaccgac gcgtgaactg aacgccgatg atgtggtgtt
    13201 ctcgttcgat cgtcagaaaa acgcgcaaaa cccgtaccat aaagtttctg gcggcagcta
    13261 cgaatacttc gaaggcatgg gcttgccaga gctgatcagt gaagtgaaaa aggtggacga
    13321 caacaccgtt cagtttgtgc tgactcgccc ggaagcgccg ttcctcgctg acctggcaat
    13381 ggacttcgcc tctattctgt caaaagaata tgctgatgcg atgatgaaag ccggtacacc
    13441 ggaaaaactg gacctcaacc caatcggaac cggtccgttc cagttacagc agtatcaaaa
    13501 agattcccgt atccgctaca aagcgtttga tggctactgg ggcaccaaac cgcagatcga
    13561 tacgctggtt ttctctatta cccctgacgc ttccgtgcgt tacgcgaaat tgcagaagaa
    13621 tgaatgccag gtgatgccgt acccgaaccc ggcagatatc gctcgcatga agcaggataa
    13681 atccatcaat ctgatggaaa tgccggggct gaacgtcggt tatctctcgt ataacgtgca
    13741 gaaaaaacca ctcgatgacg tgaaagttcg ccaggctctg acctacgcgg tgaacaaaga
    13801 cgcgatcatc aaagcggttt atcagggcgc gggcgtatca gcgaaaaacc tgatcccgcc
    13861 aaccatgtgg ggctataacg acgacgttca ggactacacc tacgatcctg aaaaagcgaa
    13921 agccttgctg aaagaagcgg gtctggaaaa aggtttctcc atcgacttgt gggcgatgcc
    13981 ggtacaacgt ccgtataacc cgaacgctcg ccgcatggcg gagatgattc aggcagactg
    14041 ggcgaaagtc ggcgtgcagg ccaaaattgt cacctacgaa tggggtgagt acctcaagcg
    14101 tgcgaaagat ggcgagcacc agacggtaat gatgggctgg actggcgata acggggaccc
    14161 ggataacttc ttcgccaccc tgttcagctg cgccgcctct gaacaaggct ccaactactc
    14221 aaaatggtgc tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta ccgacgacca
    14281 caacaaacgc gttgacctgt acaagcaagc gcaggtggtg atgcacgatc aggctccggc
    14341 actgatcatc gctcactcca ccgtgtttga accggtacgc aaagaagtta aaggctatgt
    14401 ggttgatccg ttaggcaaac atcacttcga aaacgtctct atcgaataat taaaagccat
    14461 acccggtggc gctgtgcctc tgcgtgcagc gccatccggc agcaatactt cattccctgt
    14521 ccgataaggg cgggaattga tttgtgagca atacagacac gcagttccag gctgcgggtc
    14581 actatagaga atccgggtta tgttgcagtt tattctccga cgtttgggac tcgtcatccc
    14641 cacgtttatc ggtattaccc ttctcacatt tgcctttgtc cacatgatcc ccggagatcc
    14701 ggtgatgatc atggcgggcg aacgtgggat ctccccagag cgtcacgcgc agctgctggc
    14761 tgaactcggc ttagataaac cgatgtggca gcagtatctc cattacattt ggggcgttat
    14821 gcatggcgat ctaggcattt caatgaaaag ccgcatcccg gtttgggaag agttcgtgcc
    14881 gcgcttccag gccacgctgg aacttggcgt ctgcgcgatg atttttgcta cggcagtcgg
    14941 tattccggtt ggtgtgctgg ctgcggttaa acgcggttcc attttcgatc acacagcggt
    15001 tggcctggcg ctgacaggtt attcaatgcc tatcttctgg tggggaatga tgctgatcat
    15061 gctggtttcg gtgcactgga acctgacgcc cgtctccggt cgtgtgagtg acatggtatt
    15121 cctcgatgat tccaatccgt taaccggttt tatgctgatc gacaccgcca tctggggtga
    15181 agacggcaac tttatcgatg ccgtcgccca tatgatcttg cccgccattg tgctgggtac
    15241 catcccgctg gcggtcattg tgcgtatgac gcgctcctcg atgctggaag tgctgggtga
    15301 agattacatc cgcaccgcgc gcgccaaagg gttgacccgc atgcgggtga ttatcgtcca
    15361 tgcgctgcgt aacgcgatgc tgccggtagt gaccgttatc ggcctccagg tgggaacatt
    15421 gctggcgggg gcgattctga ccgaaaccat cttctcgtgg cccggtctgg ggcgctggtt
    15481 gattgacgca ctgcaacgcc gcgactatcc ggtagtgcag ggcggcgtat tgctggtggc
    15541 gacgatgatt atcctcgtca acctgctggt cgatctgctg tacggcgtgg tgaacccgcg
    15601 tattcgtcat aagaagtaag gggacatcat gtcacaggtt actgaaaata aagtgattag
    15661 cgcaccggtg ccgatgaccc cgttacagga gttctggcac tattttaaac gcaacaaagg
    15721 cgcggtcgtc gggctggttt acgtcgtcat cgtgctgttc atcgcgatct ttgccaactg
    15781 gattgcaccc tataacccgg cggaacagtt ccgcgatgca ctgctcgccc cgccagcctg
    15841 gcaggaaggc ggcagcatgg cgcacttgct gggcaccgat gacgtaggcc gtgatgtgct
    15901 gtcgcgcctg atgtacggtg cgcgcctgtc gctgctggtt ggctgtctgg tagttgtgtt
    15961 atcgctgatt atgggcgtta ttctcggcct gatcgccggt tactttggcg gcctggtcga
    16021 taacatcatt atgcgcgtgg tcgatatcat gctggcgctg ccaagtctgc tgctggcgct
    16081 ggtgctggtg gcaattttcg gcccgtcgat tggtaacgcc gcgctggcac tgaccttcgt
    16141 tgccttgccg cactatgtgc gcttaacccg cgccgccgtg ctggtggaag ttaaccgcga
    16201 ttacgtcacc gcgtctcgcg tagcgggtgc cggggcgatg cgtcagatgt ttattaacat
    16261 cttcccgaac tgccttgcgc cgctgattgt tcaggcgtcg ctcggtttct ctaacgccat
    16321 tctcgatatg gctgctcttg gtttcctcgg catgggggca cagccgccaa cgcctgagtg
    16381 gggcaccatg ctctccgacg tgttgcagtt cgcgcaaagc gcctggtggg tcgtgacctt
    16441 cccgggtctg gcgatcctgc tgacggtgct ggcatttaac ctgatgggtg acggtctgcg
    16501 tgacgcgctc gatcccaaac tgaagcagta agaggttcga gatggcgtta ttaaatgtag
    16561 ataaattatc ggtgcatttc ggcgacgaaa gcgcgccgtt ccgcgccgta gaccgcatca
    16621 gctacagcgt aaaacagggc gaagtggtcg ggattgtggg tgagtccggc tccggtaagt
    16681 cggtcagttc actggcgatt atggggctga ttgattatcc gggccgcgta atggcagaaa
    16741 aactggagtt taacggccag gatttgcagc gtatctcaga aaaagagcgc cgcaacctgg
    16801 tgggtgccga agtggcgatg atcttccagg acccgatgac cagccttaac ccgtgctaca
    16861 ccgtgggttt ccagattatg gaagcgatta aggtgcatca gggcggcaac aaaagtaccc
    16921 gccgtcagcg agcgattgac ctgctgaatc aggtcggtat tcccgatccg gcatcgcgtc
    16981 tggatgttta cccgcatcag ctttccggcg gcatgagcca gcgcgtgatg atcgccatgg
    17041 cgattgcctg tcggccaaaa ctgctgattg ccgatgaacc gaccaccgcg ctggacgtga
    17101 ccattcaggc gcaaatcatc gaactactgc tggagctaca gcagaaagag aacatggcgc
    17161 tggtgttaat tacccatgac ctggcgctgg tggcggaagc ggcacataaa atcatcgtga
    17221 tgtatgcagg ccaggtggtg gaaaccggtg atgcgcacgc catcttccat gcgccgcgtc
    17281 acccgtatac tcaggcattg ctgcgtgcgc tgccagaatt tgctcaggac aaagaacgtc
    17341 tggcgtcgtt gccaggtgtc gttcccggca agtacgaccg cccgaacggc tgcctgctta
    17401 acccgcgctg cccctatgcc actgacagat gtcgcgctga agaaccggcg ctgaatatgc
    17461 tcgctgacgg gcgtcagtcc aaatgccatt acccacttga tgatgccggg aggccgacac
    17521 tatgagtacg caagaggcca ccctgcaaca accgctgttg caggctatcg acctgaaaaa
    17581 acattatccg gtgaagaaag gcatgttcgc gccggaacgt ctggttaaag cgctggatgg
    17641 cgtttcgttt aaccttgaac gtggcaaaac gctggcagta gtgggcgaat ctggctgcgg
    17701 taaatcgacc ctcggtcggt tgctgacgat gattgaaatg cccaccggtg gcgagctgta
    17761 ttaccagggg caggatctgc ttaagcacga tccgcaggcg cagaagctgc gtcggcagaa
    17821 aatccagatc gtcttccaga acccttacgg ttcgctgaat ccgcgtaaaa aagtcgggca
    17881 aattcttgaa gagccgctgc tgatcaacac cagcttaagc aaagaacagc gtcgggaaaa
    17941 agccctgtcg atgatggcga aagtcggcct gaaaaccgag cactatgacc gctatccgca
    18001 tatgttctcc ggcggtcagc gtcagcgtat cgccatcgcc cgtggtctga tgctcgaccc
    18061 ggatgtggtg attgccgatg aaccggtttc cgcgctggat gtttcagtgc gcgcgcaggt
    18121 gctgaatctg atgatggatt tgcagcagga gttggggctg tcttatgtct ttatctccca
    18181 cgacctgtcg gtggtggagc acattgctga tgaagtgatg gtgatgtacc tgggccgctg
    18241 cgtggagaag ggaacgaaag accaaatctt caataacccg cgccatccgt acactcaggc
    18301 gctactttcc gcgacgccgc gcctgaaccc ggacgatcgc cgcgagcgca tcaagctcag
    18361 cggtgaacta ccaagcccac tgaatccacc gccgggttgc gccttcaacg cccgctgtcg
    18421 tcggcgcttc ggcccctgca cccagttgca gccgcagcta aaagactacg gcggtcaact
    18481 ggtagcttgt tttgctgttg atcaggatga aaatccgcag cgttaactca caaaaccggg
    18541 gccatgcgct ccggtttact caatcagctt caacagtggc gacaagcata gcaaaatgcc
    18601 gtacagcaga atcagccagg ctttaaatcc gcgaagtttt tccagttgtg cgactttata
    18661 gatcaggaaa aacggaatga ggcacgacac aataccatat aacgggcttc ccaactgaaa
    18721 gaacaccagc accgatacac gaaacgaaac ccaaatcgtc aacgtaatga cgataaaagc
    18781 gcagatcgcc agagtcagca cgcgtgagtt aattttcttg gtatcaataa ttcggcttaa
    18841 cagattgaga ataatgcctt taatggcctc gtggaaaccg agataaatgc caaagaatgc
    18901 ggtcagtacg gcaaagatat taagcaccgt agaggtgata tgaatgatat gcccagggat
    18961 cacctgcgcg gccagcgcca gtgctgagat attttgttca aaggcagaaa cggcttcttc
    19021 gtggctaatt gagaaggtaa acgaaaaggc aaaaaacagg atcaccgcga tgagcgtgat
    19081 ataactaatt cggtgggtac gcagcgcgag ccgggttgcc agtactttat ccgcttcccg
    19141 tttacgatag gcaatattca ttgggttaag tacctgaata aatactgcag aaaagaagca
    19201 aaatggaatg gtaagcaaga catcgcggaa aaagacggag gcttgcggga aggcggttat
    19261 attggcgaaa ttccagtgcg ggatcatcgc aaaaccgaac acgacaataa tccctacttt
    19321 gaccaccacc attggcccgg aaatcttaaa cagtaatcgt tcaccaccag acgcaatcgc
    19381 caccagtacg gcgaaaatag cgactttata aagtagagat tgtgaaagat cggcatcggt
    19441 taaaccgaag gttttcaggt acgaggcgct gtcgaaaacc acggagagag agtagataaa
    19501 aatcccgtgg ataatcatca aaaagtagat aaccccgagg aaaattcccc agttcttccc
    19561 caggtaatga ctgataatat cggtgtagtc attacaggaa tcgctttcag aaagggtttt
    19621 taaataaatg tcctgcacta cccaggtggc aggataagca atgatcgctg cggtaataaa
    19681 tacccaaatt cccttcaagc caatttgtac tggcatcagc acggttccgg caccaatcgc
    19741 catgcctatg cataataaaa cccagccgaa gtcgtagcgt gtaaacggca atttctgatt
    19801 gtgtttcgat agtgtgttgt gctgcataaa gtcctgtcca gaaagaagtc ggcctcgcaa
    19861 aaatgaataa acccagtttc tcttgcgcaa caggtggaga agtcgaatac gcagtatgta
    19921 aaaaatgagg caacagattc actggggagg tcgcgaggag gcgcaattct acaaaattgc
    19981 gcatttcact accagtctgg agtcagcccg gatatgaact gcaactattg caaataaaga
    20041 aattaagctg gaaggcatcg cgcaatgagg aagattgccg ggtgctgaca gtaaaaagca
    20101 atcgtaaaat cagcggcaag ccggaagggt tccggtgagg cgcaatgttg cgggggcttt
    20161 atccctggtg gcattggttg ctggagagag aaaacccccg cacgttgcag gtatatacct
    20221 gacaacataa cgggggctaa tcttgaacct agaccactca agaatagccg cgaaacgttg
    20281 tcattacaac acaggcggct atatgacgct cgcagagctg ggcatggcct tctggcatga
    20341 tttagcggct ccggtcattg ctggcattct tgccagtatg atcgtgaact ggctgaacaa
    20401 gcggaagtag cgtgtcatgt gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca
    20461 ggccgcaggg gtgaaactct gcggcctttt tcgtaagaaa aatgcaacgg ttgcaagcgg
    20521 ataatattct gcggaatgcg cagcacagag tgaagatgag acggattccg gaacagaaaa
    20581 acagtcgtag aatcagcgtc aagccggaag gattccggtg aggcgcaatg ttgcgggggc
    20641 ttaatccctg gtggcattgg ttgctggaga gaaaaaaccc ccgcacgttg caggtatgca
    20701 cctgacaaca ccacgggggc cattcttgca tccaaacaat acaagaatag ccgcgaaacg
    20761 ttgtcattac aatacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca
    20821 tgatttagcg gctccggtca ttgctggtat tcttgccagt atgatcgtga actggctgaa
    20881 caagcggaag taacgtgcca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga
    20941 tcaggccgca ggggtgaaac tctgcggcct
//
LOCUS       DAOFQD010000078        20693 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.078, whole genome shotgun sequence.
ACCESSION   DAOFQD010000078 DAOFQD010000000
VERSION     DAOFQD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.078"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1630)
                     /gene="actP"
                     /locus_tag="QBF46_003013"
     CDS             complement(<1..1630)
                     /gene="actP"
                     /locus_tag="QBF46_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDQ6675169.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGV"
     gene            complement(1627..1941)
                     /locus_tag="QBF46_003014"
     CDS             complement(1627..1941)
                     /locus_tag="QBF46_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDQ6675170.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2142..4100)
                     /gene="acs"
                     /locus_tag="QBF46_003015"
     CDS             complement(2142..4100)
                     /gene="acs"
                     /locus_tag="QBF46_003015"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDQ6675171.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4493..5929
                     /gene="nrfA"
                     /locus_tag="QBF46_003016"
     CDS             4493..5929
                     /gene="nrfA"
                     /locus_tag="QBF46_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDQ6675172.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQTDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5974..6540
                     /gene="nrfB"
                     /locus_tag="QBF46_003017"
     CDS             5974..6540
                     /gene="nrfB"
                     /locus_tag="QBF46_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDQ6675173.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHATKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6537..7208
                     /gene="nrfC"
                     /locus_tag="QBF46_003018"
     CDS             6537..7208
                     /gene="nrfC"
                     /locus_tag="QBF46_003018"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDQ6675174.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7205..8161
                     /gene="nrfD"
                     /locus_tag="QBF46_003019"
     CDS             7205..8161
                     /gene="nrfD"
                     /locus_tag="QBF46_003019"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDQ6675175.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8181..9899
                     /locus_tag="QBF46_003020"
                     /pseudo
     CDS             8181..9899
                     /locus_tag="QBF46_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
     gene            9892..10275
                     /gene="nrfF"
                     /locus_tag="QBF46_003021"
     CDS             9892..10275
                     /gene="nrfF"
                     /locus_tag="QBF46_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDQ6675176.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALKIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10272..10868
                     /gene="nrfG"
                     /locus_tag="QBF46_003022"
     CDS             10272..10868
                     /gene="nrfG"
                     /locus_tag="QBF46_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDQ6675177.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11210..12523
                     /gene="gltP"
                     /locus_tag="QBF46_003023"
     CDS             11210..12523
                     /gene="gltP"
                     /locus_tag="QBF46_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDQ6675178.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12602..13291)
                     /locus_tag="QBF46_003024"
     CDS             complement(12602..13291)
                     /locus_tag="QBF46_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDQ6675179.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENGSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13385..15532)
                     /gene="fdhF"
                     /locus_tag="QBF46_003025"
     CDS             complement(13385..15532)
                     /gene="fdhF"
                     /locus_tag="QBF46_003025"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15113..15115),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDQ6675180.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNHGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15730..17196)
                     /gene="mdtP"
                     /locus_tag="QBF46_003026"
     CDS             complement(15730..17196)
                     /gene="mdtP"
                     /locus_tag="QBF46_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDQ6675181.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17193..19244)
                     /gene="mdtO"
                     /locus_tag="QBF46_003027"
                     /pseudo
     CDS             complement(17193..19244)
                     /gene="mdtO"
                     /locus_tag="QBF46_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(19244..20275)
                     /gene="mdtN"
                     /locus_tag="QBF46_003028"
     CDS             complement(19244..20275)
                     /gene="mdtN"
                     /locus_tag="QBF46_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDQ6675182.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20294..20569)
                     /locus_tag="QBF46_003029"
     CDS             complement(20294..20569)
                     /locus_tag="QBF46_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675183.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4664 a         5497 c         5658 g         4874 t
ORIGIN      
        1 caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca cgctcgcgcg
       61 ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat gccacggtga
      121 tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgacca aggatctgta
      181 cccaaatcgt cgggccgagg atcatcagta ctactgcggt aatcagcccc agccagccac
      241 ccatcatcgc gccacgcgtg gtcagtttcg accagtacat agaaagcaga atgatcggga
      301 agttacagct cgccgcgatg gcaaacgcca gccccaccat aaaggcgatg ttctgattct
      361 caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg atttttgata
      421 cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac aagtcatgcg
      481 aaaccgcgga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata gtggcgaaag
      541 caaccgctga aataaaaccg aggaacaggt tgccgcccac tgcattcgcc aggtgaacgg
      601 ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc ggattcgcac
      661 caaccagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag tagcccataa
      721 acccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc
      781 gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagaccc agagagagcg
      841 cggagatcgg atctttcacc agcccgcccg gcttcatgat gtcgacacct ttcgggtgta
      901 ccgccatcgc ttcactgaac agattgttga agctaaagcc gacgtgtttc atcaccataa
      961 aggccataaa gctggcaccg aacagcaaca gcacggcttt gataatttgc acccaggtgg
     1021 tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc agcaccaccg
     1081 caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct accatctggg
     1141 cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata cgaatcggcc
     1201 cttgtttcag gcggtaagag gctacatcgg caaaggtgta gcgtcccagg ttacgcagac
     1261 gttcggcaat caggaacaaa atgatcggcc agcccaccag gaagcccagc gagtaaatta
     1321 agccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag gcggcggaca
     1381 tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg cctgcggtgt
     1441 agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg agcgtaaaca
     1501 cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc tctacggccc
     1561 cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc gccgtcagaa
     1621 ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt cgaattcgcc
     1681 gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca ctccaacacc
     1741 aatcggaata ccgcgtgtga cgctggtgtt cggattcagc ggggtgccca gccagccggg
     1801 cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa tcgacaggat
     1861 ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt cttctatccg
     1921 ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat tgttttaatt
     1981 cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg taggccggat
     2041 aaggcgttat acgccgcatc cggcaatcaa tgcctgatgc gacgctgtcg cgtcttatca
     2101 ggcctacaaa ccgttaccga ctcgcatccg gcaattgtgg gttacgatgg catcgcgata
     2161 gcctgcttct cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg
     2221 cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg
     2281 gagcgggttt taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca
     2341 atctctttac gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg
     2401 tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact
     2461 acggcggctt cggcaatctt cggatgcgcc accagcgccg actcaatctc tgccgtcccc
     2521 agacggtgac cggagacgtt cagcacgtcg tccacacgcc cggtgatcca gtaatagcca
     2581 tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag
     2641 gtctgttcaa aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg
     2701 gtgatcacca ggctgccttc ggtagcgcct tccagcgggt taccttcgtt atcgaccagc
     2761 gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg ctttcagctc ggtagcgcca
     2821 ggcagcggcg tgatcatgaa accgccagtt tcggtctgcc accaggtatc gaccaccgga
     2881 catttctcgt tgccgatttt tttccagtac cactcccacg cttccgggtt aattggctcg
     2941 cccacggaac cgagaatgcg cagcgacgaa cggtcggtac cctcgatcgc tttatcgcct
     3001 tccgccatca gcgcgcggat cgccgtgggt gcggtataga gaatattgac ctgatgcttg
     3061 tccaccacct gcgccatacg ggcaggcgtc ggccagttgg gtacgccttc aaacatcagc
     3121 gtggtcgcac cgcaggccag cgggccgtac agcaagtaac tgtgtccggt cacccagccc
     3181 acatcggcgg tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc
     3241 agcgccgcgt acaccagata accgccggta gtatgcagca caccttttgg cttaccggta
     3301 gaaccggagg tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc
     3361 tgatcgctcg cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtca
     3421 attttcccgc cagtacgctt cagtaccacc acatgctcta cgctggtgac gttcgggttt
     3481 ttcagcgcgt catcaacgtt tttcttcagc ggaatactgc gcccggcacg cacaccttcg
     3541 tcggaagtga tcaccagtcg tgagttggaa tcaataatgc gcccggcaac ggcttccggc
     3601 gagaagccgc cgaaaatcac cgaatgcacc gcgccaatgc gggcgcaggc cagcatcgca
     3661 accgcggctt ccggcaccat cggcatataa atcgccacca catcaccttt tttaatgccc
     3721 agctcgagca gggtattggc gaagcggcag acgtcgcggt gcagctcttt atagctgata
     3781 tgtttgctct ggctggcgtc gtcgccttcc cagatgatgg cggtacgatc gccgttttct
     3841 tgcagatggc ggtcaaggca gtttgccgcc agattcagcg tgccgtcctc gtaccattta
     3901 atggacacat taccgggggc aaaggaggtg tttttcacct tctggtaagg tttgatccag
     3961 tcaagaattt ttccctgttc gccccagaag gtatcaggta cgttaataga ttgttgatac
     4021 atcgcctcgt actgctgagg gtttatcagg caacggtctg cgatgttggc aggaatggtg
     4081 tgtttgtgaa tttggctcat gcttttgttc tccttgtagg atgttaataa tatgtggcat
     4141 aagcgttaaa tgtaggggta ttggcagttt tgtttagtat ttgggcgaca gatcacgcaa
     4201 aagtagaatt gtgcaaataa acggcagggt aatttttgaa ggtcaccaac aaaagttgat
     4261 taattctttg aggaacatgc agttatgcat gctgttgaaa aagaggaaga tactgactaa
     4321 ctctaaagtg gtattttaca tgcacttaca attgattaaa gacaacattc acagtgtggt
     4381 tatttgttac acataggggc gagcaatgtc atgacagtgt aggtgcggtt actgtcgtga
     4441 aaaacaataa aaaccgccat tgcaacaatg gcgcaattcg gatgaagccc ctatgacaag
     4501 gataaaaata aacgcacgcc gtatcttcag cttattgatt ccttttttct ttttcacttc
     4561 tgttcacgct gaacaaacgg cagctcccgc aaaacctgta actgtggaag cgaagaatga
     4621 aacctttgcc ccgcagcatc ccgatcaata tctctcctgg aaagccacct cggaacagtc
     4681 agagcgtgtt gacgccctgg cggaagatcc acggctggtg atcctgtggg cggggtatcc
     4741 cttctcgcgc gattacaaca agccgcgtgg acatgccttt gctgtgaccg atgtgcgtga
     4801 aaccctgcgt accggtgcgc cgaaaaacgc tgaagatggt ccgctaccga tggcatgctg
     4861 gagttgtaaa agcccggatg tggcgcgtct gatccagaca gacggcgaag atggctactt
     4921 ccacggaaaa tgggcgcgcg gcggcccgga aatcgtcaac aacttaggtt gtgccgactg
     4981 ccataacacc gcctcaccag agttcgccaa aggcaaaccg gagttaaccc tttcccgtcc
     5041 gtatgcggct cgcgcgatgg aagccattgg taaacctttt gagaaagccg gacgtttcga
     5101 ccagcaatcg atggtttgcg gtcagtgcca tgtggagtat tacttcgacg gcaaaaacaa
     5161 agcggttaaa ttcccgtggg atgacggcat gaaagtcgaa aatatggagc agtattacga
     5221 caaaattgcc ttctctgact ggactaactc cctgtcgaaa acgccaatgc tgaaagcgca
     5281 gcacccggaa tatgaaacct ggacagcggg cattcacggt aaaaacaacg tgacctgtat
     5341 cgactgccat atgccaaaag tgcagaacgc cgaaggcaaa ctctacaccg accataaaat
     5401 tggtaatccg tttgataact tcgcccagac ttgtgcgaac tgccataccc aggacaaagc
     5461 tgccttgcaa aaagtggtcg cggaacgtaa gcagtcgatt aacgacctga aaatcaaggt
     5521 tgaagatcaa ctggttcacg ctcacttcga agcgaaagca gcgctggatg caggcgcgac
     5581 ggaagctgaa atgaagccaa ttcaggacga tatccgtcat gcccagtggc gctgggatct
     5641 ggcgatcgct tcccacggca ttcatatgca cgcaccggaa gaaggtttac ggatgctcgg
     5701 tacggcgatg gataaagcgg cggatgcacg caccaaactg gcgcgcctgc tggcgaccaa
     5761 aggcatcacc catgaaatcc agatcccgga tatctcaacc aaagagaaag cccagcaggc
     5821 cattggcctg aacatggaac aaatcaaggc cgagaagcag gacttcatca aaacggtgat
     5881 cccgcagtgg gaagaacagg cacgtaaaaa cggtctgtta agccaataac cccgttccgc
     5941 ctcacaaggg gcggaaaaca caatggagtg aatatgagcg tattacgttc gttattaact
     6001 gctggggtgc tggcgtcggg cctgttgtgg agcctgaacg gaattaccgc tacccctgcg
     6061 gcgcaggcat ctggtgatcg ctacgaagtt acccagcagc gtaacccgga tgccgcctgt
     6121 ctggactgtc ataagccaga taccgaaggt atgcatggca aacatgcttc cgttatcaac
     6181 ccgaataaca aactgccggt cacctgcacc aactgtcacg gccagccatc accgcaacac
     6241 cgcgaagggg tgaaagatgt gatgcgcttt aacgagccga tgtacaaggt tggagagcag
     6301 aacagcgtct gtatgtcctg tcatctgccg gaacagttgc aaaaagcgtt ctggccgcac
     6361 gatgtccacg caaccaaagt ggcgtgcgcc agctgccatt ccctgcatcc acagcaagat
     6421 acgatgcaga cgttaagtga caaaggacgg attaagattt gcgtcgattg ccacagcgat
     6481 cagcgcacca atccgaactt taacccggcg tctgttccgt tgcttaagga gcagccatga
     6541 cctggtctcg tcgccagttt ctcaccggcg tcggcgtgct tgcagccgtc agcggcaccg
     6601 caggacgcgt ggtggcgaaa acgttgaata tcaatggggt gcgttacggc atggtgcatg
     6661 acgagtcgtt atgcatcggc tgtaccgcct gtatggatgc ttgtcgggaa gtaaacaagg
     6721 tgccggaagg cgtctcgcgc ctgacaatta ttcgtagcga gccacagggc gaatttcctg
     6781 atgtgaaata tcgcttcttc cgtaagtctt gccagcactg cgatcatgcg ccgtgcgttg
     6841 acgtctgccc gaccggtgcg tcttttcgcg atgctgccag tgggattgtc gatgttaatc
     6901 cggatctctg cgtcggttgt cagtactgca tcgccgcctg tccgtaccgc gtgcgcttta
     6961 tccatccggt cacgaagacg gcggacaaat gcgatttctg ccgtaagacc aatttgcagg
     7021 ccggtaagct gcccgcgtgc gttgaggctt gcccgaccaa ggcgctgacg tttggcaatc
     7081 tggacgatcc taacagtgag atttcgcaac tgctgcgcca gaagcctact taccgctaca
     7141 agctggcgct gggaaccaaa ccgaagctgt accgcgtacc gtttaaatac ggggaggtga
     7201 gccaatgacg cagacttccg catttcattt tgaatcactg gtgtgggact ggccgattgc
     7261 catctacctg tttttgattg gtatttctgc cggtctggtg acgctggccg tgctgttacg
     7321 tcgcttctac ccgcaggcgg gcggtgcaga cagtacgttg ctgcgcacca cgctgattgt
     7381 cgggccgggc gcggtgatcc tcggtctgtt gatcctcgtc ttccacctga caagaccgtg
     7441 gaccttctgg aagctgatgt tccactacag ttttacctcg gtgatgtcga tgggggtgat
     7501 gctgtttcag ctctacatgg tggtgctggt gctgtggctg gcgaaaatct ttgaacatga
     7561 tttgcttgcc ctgcaacaac gctggttgcc gaagctgggg atcgtgcaaa aggttctgag
     7621 cctgctgacg cccgttcatc gcggactgga aacattgatg ctggtgctgg cggtgctgct
     7681 gggggcttat accggctttc tgctatcggc gctgaaatcg tatccgttcc tcaataaccc
     7741 gatcctgccg gtgttgttcc tcttctccgg catctcgtcc ggtgcggcgg tggcgctgat
     7801 cgccatggcg atacgccaac gcagtaaccc gcattccacg gaagcgcact ttgtacaccg
     7861 gatggaaatc cctgtggtat ggggcgaaat cttcctgctg gtggcgtttt ttgtcggact
     7921 ggccctgggc gatgacggta aagtgcgtgc gctggtggcg gcattgggcg gcggtttctg
     7981 gacgtggtgg ttctggcttg gtgtcgccgg gctggggctg attgtgccaa tgttgctcaa
     8041 accgtgggtc aatcgcagtt ccggcattcc tgctgtgctg gcggcgtgtg gggccagcct
     8101 ggtcggcgtg ttgatgttgc gatttttcat tctctacgcc gggcagttaa cggtggcgta
     8161 agccagaaca gaggtggttt ctggacgtat tccttcctga agtcggtttt ctggcgttgt
     8221 tgttaagtct cggggtcaac gtgttgaccc cgttgacggc ctttgcggga gtgcggttgc
     8281 gctggcctgc catgatgcga ctcacttgca tcggcattct ggcgcagttc gcgctcctgc
     8341 tgctcgcctt tggcgtactg acgtactgtt ttctcatcag cgatttctcg gtcatttatg
     8401 tcgcgcaaca tagctacagc ctgctgtcgt gggaactcaa actggcggcg gtatggggtg
     8461 gtcatgaagg ttcgctgttg ctttgagtgc tgctgctttc cgcctggagc gcgctgtttg
     8521 cctggcatta tcggcagcaa accgatccgc tatttccgct gacgctagcc gttttatctc
     8581 tcatgcttgc cgcactgcta ctgtttgtgg tgctgtggtc cgatcccttc gtgcggatat
     8641 ttccaccagc aatcgaaggc cgcgatctca atccgatgct gcaacatccc ggtcttatct
     8701 tccatccgcc gctgctttat ctcggctatg gcggtttgat ggtggcggcg agcgtggcgc
     8761 tggggagttt actgcgcggc gagtttgatg cggcctgcgc ccgaatttgc tggcgctggg
     8821 cactacctgg ctggagcgca ttaacggcgg ggatcatcct cggttcctgg tgggcctatt
     8881 gcgaactggg ctggggcggc tggtggttct gggatccggt ggaaaacgcc tctttattac
     8941 cctggctttc tgccactgcg ctgctgcaca gtttgtccct gacacgtcag cgggggattt
     9001 tccgccactg gtcgctgctg ctggcgatag ttactttgat gctgtcgctg ctgggcacct
     9061 taattgtccg ttctggcatt ctggtttcgg ttcatgcgtt cgcactggat aacgtccgcg
     9121 ccgtgccgtt gttcagcctg tttgcactga ttagccttgc gtctctggct ctgtatagct
     9181 ggcgagcgcg ggacggtggc ccggcggtgc gtttttcggt gttatcgcgg gaaatgttaa
     9241 tcctcgctac gctgttgctg ttttgcgcag tgctactgat cgtgctggtg ggaacgcttt
     9301 atccgatgat ttacggtctg ctgggctggg gacgcctctc cgttggcgcg ccgtatttta
     9361 accgcgcgac gttaccgttt ggtctgttga tgctggtggt gattgtgctg gcgacgtttg
     9421 tctctggcaa acgcgtgcag cttccggcgc tggtagctca tgctggcgtg ctgttatttg
     9481 ccgcggggat cgtggtttcc agcgtcagtc gtcaggagat cagcctgaat ttacagccgg
     9541 atcagcaggt gacgctggca ggatacacct tccgttttga gcgactcgat ctgcaagcca
     9601 gaggcaatta caccagcgaa aaagcgatag tggcactgtt tgaccatcag caacgtattg
     9661 gtgaactgac gccggagcgg cgtttttatg aagcacgccg tcagcaaatg atggaaccgt
     9721 caattcgctg gaacggcatc catgactggt atgcggtcat gggggagaaa actgggccgg
     9781 atcgttacgc ttttcgtttg tatgtacaaa gcggtgtgcg ctggatctgg gggggaggat
     9841 tgttgatgat tgcaggcgca ttattaagcg gatggcgggg gaggaagcgc gatgaataaa
     9901 ggacttctca cgctactgct gttatttacc tgttttgcgc gcgctcaggt cgtagacacc
     9961 tggcaattcg ccaatccgca acaacagcaa caggcgttaa aaattgccag ccagttacgt
    10021 tgtccgcagt gccagaatca aaacttactg gaatccaacg cgccggtggc agtcagtatg
    10081 cgccatcagg tttacagcat ggtggcggag gggaagaacg aagtcgaaat cattggctgg
    10141 atgaccgaac gctacggaga ttttgttcgc tataacccac cgttaaccgg tcagacgctg
    10201 gtgttgtggg cgctgccagt ggtgttgtta ctgctgatgg cactgatcct ctggcgagtg
    10261 agggcgaagc gatgaaacag cccaaaatac cggtgaaaat gctgacaaca ctcacgattt
    10321 tgatggtatt tctctgtgtc ggcagttatc tgttaagtcc aaaatggcag gcggtacatg
    10381 cggaatatca gcgtcagcgc gatccgctac atcagtttgc cagccagcaa accccggaag
    10441 cgcagcttca ggcattgcag gataaaatcc gcgctaatcc acaaaacagc gaacaatggg
    10501 cgttactggg cgagtactat ctgtggcaaa acgattacag caattcgctg ctggcgtacc
    10561 gtcaggcgtt gcaactacgt ggtgagaacg ctgaactgta tgcggcgctg gcgacggtgc
    10621 tttattacca ggccagccag catatgaccg cccagactcg cgcaatgatc gacaaagccc
    10681 tcgcgctgga ctctaacgaa atcaccgctc tgatgctgct ggcttccgat gcgtttatgc
    10741 aggcgaatta cgcgcaagcc atcgaactat ggcaaaaagt gatggatctc aactcacccc
    10801 ggatcaaccg gacacagctg gtcgagtcga ttaatatggc gaaattgttg cagcggagat
    10861 cagattaatc atctggtgat gaagattatg ttattgagtt gtataaaaaa taatcgaatg
    10921 aacatatgcc aaaaataatc accaatcagt attattgcag attaaacaaa taaaaatctt
    10981 tccataacaa atggttattc attaattctg ccataaactc gcgttattat gcattaatgc
    11041 agcgaaaagc tctgttgtta aagggttgcg caacataccg cgcaaatgat actgatcata
    11101 agcgttaaaa aaatctacaa accaacgcaa cacaattcat gccctggcag tatgtcacgt
    11161 tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaagtat
    11221 aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag
    11281 ctacctgcac taccatagcg acagccgcga ctggctggtc gtcaatttgc tctctccggc
    11341 gggtgatatc ttcatccatc tgatcaaaat gattgttgtg ccgattgtga tctccacgct
    11401 ggtggtgggt atcgcgggtg ttggtgatgc caaacagctc gggcgtattg gcgcgaaaac
    11461 cattatttac ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa
    11521 cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa
    11581 atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt
    11641 gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat
    11701 ctttttctcg gtgctgtttg gtctggggct ttcttccctg cccgcgacgc atcgtgaacc
    11761 gctggtgacc gtgttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat
    11821 gcgttatgca ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt
    11881 ctcttctctg tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt
    11941 cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat
    12001 tcgtattctg aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtact
    12061 gccgcgaatt attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagctttgt
    12121 ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc
    12181 tattttcatc gctcagctgt atggcattga cctgtccatc tggcaggaaa tcattctggt
    12241 gctgacgctg atggtgacct cgaaagggat tgctggcgtg ccgggcgtgt cgtttgtggt
    12301 gttgctggca acgctgggta gcgtaggtat tccgctggaa ggtctggcgt ttattgctgg
    12361 tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc
    12421 ggtgctggtg attgccaagt gggaacacaa atttgaccgc aaaaaggcgc tggcttatga
    12481 gcgtgaagtg ctgggaaaat ttgataaaac tgcggatcaa taattgaaga ttgccgggga
    12541 tatccacccg gcaatgtgtg aatgcctgat gcgacgcttg ccgcgtctta tcaggcctac
    12601 cttaaccgtt agttaatttt tcaaactctt cacacccggt atcaaaccct tccatacagc
    12661 tcaggttcaa ccagtgtagc gccttttgtt tgttcttctc gataaaacct tcttcaccgt
    12721 ttaagaacat cattcccgcc cagtactcgg aataaccggt tcgggaaatt gcggagctgc
    12781 gtttgaaata ccaggttgcc ttgtcatcgt cggccttaat gcccacgccg ttggcgtaaa
    12841 tcaaaccaag cagcatttgg gcatcgacgg cagagccgtt ctccagatct tccgaggcgt
    12901 tttctaacag cgaaatcgcc tttggataat ccggtttacc cgcttgagta tttaccagaa
    12961 tatgcgccag cgttacttca ccttctttac tgcccgcttg cgccgctttc tctgcaagaa
    13021 cttttgcttg tggatagtcc agactgaccg gattggtgat tttgatctgc gccaacagcg
    13081 cgcaggcatc agcatcaccg ctgtcggcgg ctttctgtgc ccaatattcg gctttgctca
    13141 aatcgccgga gctaaaccag ctgtcggcaa gaaaatattg tgcgcgtcgg tccccggcct
    13201 ctgctgcctt taaatactgg ctgccaggct cggagtcgtt ggcgtgggca aagaatgtca
    13261 aaaacaacat caatgcgata atttttttca ttttgataat acgattgggt agggagtaac
    13321 cagtataaag agttcgaatg gataaaaaaa cagcctccga aaggaggctg tagaaaggac
    13381 tgtattacgc cagtgccgct tcgcgcaggc gagttttcaa cttgttgtac tcgtcaatca
    13441 cgtactgctc ggcggcgcgc tgatcggcga tcggctcgac gcgaacggcg cagtatttgt
    13501 actccggcgt tttcgtaatc gggcttaagt tttcggtaac cagctcgtta caggcaccaa
    13561 tccaccactg gtaggtcatg taaatcgccc ctttgttcgg acgatcgctg acctgcgcac
    13621 gggtgataat tttgccttta cgcgagtgca cccaaaccaa tgcctcatct tcaataccca
    13681 gacgtttggc gtcttcggta ttgatttgtg cgtagccagg ttcatcagcc agcgccgcca
    13741 gtgccgcaca gttaccggtc atcgaacggc aagagtagtg accaacttca cgcaccgttg
    13801 acagtaccat cgggtactcg tcggtgagtt tgtcgattgg cgctacccag tcgcaggtga
    13861 agaactgcgc cagaccgttc ggggtatcaa acttctcttt aaacagataa gaagtcccct
    13921 gatcggcatc tgaagtatcg cggcaaggcc actgaatgaa gcccagttcg cccattttct
    13981 cgtaagtcgc accgtagaaa tccgggcaca gatgacgcaa ctcatcccag atctcctggg
    14041 tgttgttgta gtgcatcgga taacccatac gggtggcgat ttcactgatg atttgccagt
    14101 ccgttttcag atcccatttc ggttcaaccg ccttgaagaa acgctggaag ccacggtcag
    14161 ccgcagtaaa cacgccttca tgctcgcccc acgacgttga cggtaaaata acatccgccg
    14221 ccgacgcggt tttggtcata aagatgtcct gaacgataac cagttccaga tcttcaaagg
    14281 ctttacgtac tgccgacagc tccgcgtcag tttgcagcgg atcttcgccc ataatgtacg
    14341 cggcacgcac ttcgccatgc gctgcgcggt gcggtagctc gctgatgcga tagccggtat
    14401 gcgctggcag gctttccacg ccccaggctt tggcgaattt ctcgcggtta gccggatctt
    14461 tcacgtactg gtatcccgga tacgtatccg gcagcgcgcc catatcgcag gcaccctgta
    14521 cgttgttctg accacgaacc gggttaacac ccgcatgtgg cttaccgagg ttaccggtca
    14581 gcatcgcgag gctggtcaga gaacgcacgg tttccacgcc ctggtagaac tgggttacac
    14641 ccatgcccca caggatggcg gcgcttttcg cctgggcata catccgtgcc gcctgacgaa
    14701 tctcactggc gctgacgccg gtgatatctt caaccgactc cggcgtgtag ccttcaacga
    14761 ttttacgata ctcttcaaag ccttctgtac gggaagcgac gaatgctttg tcgtacaaat
    14821 tttcttcaat aatgacatgg cccatcgcat tcaacagcgc gatgttcgag ccgtttttca
    14881 gtgcaatgtg catgtcagca atgcgcgcgg tttcaatttt gcgcggatcg cagacgataa
    14941 ttttcgcccc gttacgttta gcgttaatta cgtgattcgc tacgattggg tgggaatccg
    15001 ccgggttgta cccgaaaacg aacactaaat cggtattatc aatttcgtta atagcattgc
    15061 tcattgcgcc attaccgacc gattggtgca gacctgcaac cgatgggccg tgtcagacac
    15121 gagcgcagca gtcaacgtta ttggtaccaa taacggcgcg cgcaaatttt tgcattacat
    15181 agttggtttc gttacccgta ccacgcgagg agccggtcgt ctggatggca tccggaccgt
    15241 acttctcttt gatggcgctc aggcgctcgg caacgtaatt cagtgcctca tcccaggaaa
    15301 caggttcgag tttgccgcca cgctggcgac ggatcatggg ggttttcagg cgcggggtca
    15361 ggatctgggt atcgttaatg aaatcccagc cataataacc cttcagacac agggtaccat
    15421 ggttggtttt cccctgcgct gcctccgccc ggacgatttt gccgttatcg acgaccaggt
    15481 tgattttgca acctgatgcg caataggggc aaaccgtgac gacttttttc atcggtctcg
    15541 ctccagttaa tcaaatcacg catacgcgct ctcgactaca gtatgcatct tttatgccac
    15601 attttatttg gggtcattcc ctgatattac gggcactatt tattcaaaac tctgacgaaa
    15661 aacaggctgt cgtcagtttt gacgtgacga aacgaaatac cgcgtgacag ccatcacgcg
    15721 gcagacattt tattttttct cgacgacggg agccgcctga tacccgccgc ccagcgattt
    15781 catcaactga atgctttgga tcacccggcg gctgtccagc atcagtaatg acatctcttc
    15841 ggcaagtact ggcaaccggg cttcagtggc ctgtaagcgg ctggttaagc cgcgctgata
    15901 ggcggcctcg gcagcgcgct gggtgaagcg cgttgcttcc acgcgttcag cctgcatttc
    15961 tcgttcgtcg ttgagcgttt gcagacgcgt gccgttgacg gcaacgtcac gcaccgcgtt
    16021 cagtactgac tggttgtaac gttcaatcat catgttgctg gcggcgcgcg taccttcgag
    16081 attggcattc aaccgtccac cgtcaaacag cggcaatttc agacccggga tgaagttgaa
    16141 ctggcgactg gtttttttga ataaggtatc caggtggatg gagtccagac cgaaaaacgc
    16201 tttgatatca aagcttgggt agaacagcgc ccgcgcggaa tccacctgat ctaatgacgc
    16261 ctgaacatac cagcgcatgg cttgcagatc cgggcgtctg gcgagcaact cataagagag
    16321 tgtcgccgga atgccggtct ggacttgcgg taatgccacc ggtttgatct ccggcatatc
    16381 gctggctccc gcgccaatca atgcacgcag agattctcgc gtttcggtga tttgcccttt
    16441 gacggcagca atttgtttat cgaccgccag aatctgtgcc cgcgcgccgt ggaaaggcac
    16501 ttgcgcttcc agaccgtgcg ccactttact ctggtgcgct ttcaccgcgt aatcaatcac
    16561 atcgcgagtt tgttctaaca gatcgagcat ctgatagctg gcctgcatac tgtaataaag
    16621 ctgcgctacg cccgtggtca gcgatagctc tactgctgcg gtttctgcca gcgcggcatt
    16681 atgcgcgcca atggcggcgg caaccgctga gcgatgcaca ccccacaaat caagatccag
    16741 tccggcaaac aaaccgactg tggcttccgt atagtacggc ccgtccatcc ccagtgctgg
    16801 cgcatccatc gcataagggc ttaaaaagcc gttcgccgag acgcgttggc ggttgagcat
    16861 ccctaacgct gcgacctgca actgtgaacc ggcatctaac aaatcggcct gggactgcgc
    16921 tttttcttcc cgcagtttcg cttcggcgag ggtgtgtgaa ccacttagcg tccgttggat
    16981 cagcgcatcc agctgcgggt cattgagttg tttccaccac tgcgcctgcg gccagccgga
    17041 gctggcaaga tgaatatcgt cggccagttt gatttgttcc ggtttgagct gttgatgagg
    17101 tgcagaatcc ttacgtacca gggcacagcc ggaaatcagc gtcgtgctgc cgagaatgct
    17161 gcacaacagc agacgtgaaa gttgacgatt gatcattgcg tggctccttg tgcctgttcc
    17221 gtggcgggcg ttaatgccgg ggctgtccag tctggcaagc gggcgaaaag ctggcagacg
    17281 tgttgctcct gttttaataa ggtgggcagg atggcctgag aggcgggtgt ctcttcaagc
    17341 gttatttgag gagaacggct gagtgcggtt gccagaccgg cagcgtattt ctccagggcg
    17401 tcggcaaact gacctgctct gtccggctgt agtgcgttat ccagcgcctg cgccgagttc
    17461 caggtggcat cccaggcgtg aagaagatcg cggccctgac gaataaccgt ttgcgaacgt
    17521 tcaatcagta atgcgcgttc ttcgctgtcc agttgacgct ccagcgccac gcgttggcac
    17581 atctcttcac aggcattaaa cgccgcatgc agacctatac gaatttgcag ataagtgcgc
    17641 agagccgtga cttcctgctg gcgtgggatc cgcattactt tacttaacat acccagcgcg
    17701 ccagccagtt tttgcggcag cgtgcgcgct tcactctcag gccagacaaa ggtgtaaatc
    17761 accgcggaca ccacggtacc aatgatgata cccagggcgc gatcgcgaat ttccaccagg
    17821 tcgtacaccg ggccaaaaac gttttcgagc gtggcgagcg cgaaggtgac caccatctgt
    17881 gtgccgatat aagaagaacg ttcagagctg gtggcaatcc atgcgcccaa caggaaaata
    17941 ggtgccagca caaacagcaa ttcgacaata ttgtccagct agggcatgac cagtagcgtg
    18001 aataacagcg ccagaatcgc gccgcaaaaa gccccgccaa aacgcagcac catcttctgg
    18061 tacgacgaac cgacattcgg gttagcgacg atcacgcagg tcagcataca ggtgtgaatg
    18121 ccttcccagt cgacgccgct gtagaaggtg taacagatca aacaggcgag cagcgttttt
    18181 accgcgtagc gcatatagtc tggattggta aaagcatcgg cggccattga tggtggtttg
    18241 gcggcgggcg ttggcggcgt attcgggtcc atctgaccca gttgtaacaa cgtctggcag
    18301 atattctcca ggttacattc ccgtgccgcc atcgcttcac tttcactgat ccgccagtcg
    18361 ctttgccagc actgaccttc ggcaatggaa tgctgcagct tgttgatttc tgaggctaat
    18421 ttttgtcgga attcaataat tgcctgagaa tcagcaaaag aggtgggatc gtagcgattc
    18481 agcgtcgagt aaatgtaggt taccgttgcc acgcagcttt gccaccatgc gttttgagtt
    18541 cgccagttgg catcgtccgc gaggcaaaag acattgagtt tttgtagcgc cagcgcctct
    18601 ctttcaatcc gcgtttcggg tagcggtgcg aggctgtctg tcaggtgact aatggcatca
    18661 tcaagccgat cattaagcgc ctgatgcatt tgcgaaatgg cacgactggg aaaccacagc
    18721 acgccgatta acgtcatcag caaggttgga tagaggccaa caacgataca ccacagcgtt
    18781 aagcgcacga ccacttccgg atagtcgagc atggcgggga aggtttgccc gtaaatagcg
    18841 acaatggcga cggcgaaaaa gaccagcccc aagcgatggg tgcgcatcaa aaacatgcag
    18901 cccatcagga tcggtccggc gatgatcaat cggatcaacg gttcgccgta tgaccatttg
    18961 tagatcaaaa acaggctgcc gatctccagc accgtggcaa ccacaaacaa gatcgcgaca
    19021 aatttggtgt aaaacgcgtt cgactgaata ccgtaaaaca gcactgccag cgataacgcc
    19081 acaaaaggga tctcaaaggt catcgagatc agaatcacca gcaggcagcc tacccagagt
    19141 tgcacggtct gcggcatgcg acctggtcgc cgctcgctta actcttcatg aaagaacgcc
    19201 agcagcctga ccaccggtaa tggcagggag ttgagcgcgc tcattattgc ggctcaagat
    19261 tagcgactgc cgaagcgccg atgcggaaca tttccgggtc aggtttatcg accatgattt
    19321 tgaccggaaa acgctgggca acgcggaccc agttaataga acgagacact ttcggcaggc
    19381 cgcccagcac caggccgccg tcatccggta gcacgccgta gccaatcgaa tccactttac
    19441 cctcgaaggt tttgccgctg tcactcatca ggcgaatcgt tgcgggtgta cctgagcgaa
    19501 tatttttcag atcggtttcg cggaagttgg cgatcacata ccagtgacga gtgtcgatta
    19561 gggtaaaaat agggcgcatg gcagaagcaa attgcccgac ggaggttttg agggaaatga
    19621 cccggccatc aaacggcgcg cgaacggtcg ccatttccag atgcagtttg gtcagggcaa
    19681 tatccgcttc gaccgccgca cgctgggcaa ctaatgcatc cacgccgctg acggcgctgg
    19741 cggctgactg cgcctgtaac aatacggcat taagatccgc ttctgcggcg cgctgcgccg
    19801 ttcttgcacg gtcaacatct tccgctgaga caaaaccttc tttcagtaat ggctcggtgc
    19861 ggcgtaatgt atctgtggcc tgtttcgcgg cggcacgggc tttttctacc gtggcattaa
    19921 ccgagtcggc accaaactgt tgcgcgtcaa cgctacgctg ggtgagcata atttgcttat
    19981 ccagcgccgc gagggaggct tcagctttcg ccagattggc ttcgtacggg cgcgggtcga
    20041 tgcggaacag caaatcgccc tgtttgactg cctggttgtc ggtgaccgcc agttctacaa
    20101 tgcggccgct gacttccggt accacatcaa tggtatctgc tgacgcgtaa gcgtcattag
    20161 ttgatggcgc actgtctacg cgccagataa cgaaaacaag ggcaaccagc gccaacgcaa
    20221 ccactaacag agcagggaat ttactgcgag gagctttttt cggcgtactt tccatgagcg
    20281 cgtctcaaat agattagaaa aatgccagcc agaacagcat ggcgtagaga gcaaaaaggg
    20341 cggtataaat aattccgaca aatgccagat tgatatttgc ccgctgaata actcgcctcg
    20401 tgatgagcgt caaaataagg ctggcaatgg cgcagaaaaa ccagctggga taataagcgc
    20461 cgatcaccgg gattgccgga gataatgagc atcccgaaac catgaagacg ggaattatcc
    20521 aggctgtttt tttgagttgc atcgccatgc gagagagaac tgttggcata ttaaatacat
    20581 cctgtctgct gcgaacaata tctgtagtga atatcaatat ccattgaagg cgtcagtatg
    20641 gctattttct tgatgaataa aaatagcttt attcagccac cgcattcata act
//
LOCUS       DAOFQD010000079        20526 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.079, whole genome shotgun sequence.
ACCESSION   DAOFQD010000079 DAOFQD010000000
VERSION     DAOFQD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.079"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..334)
                     /locus_tag="QBF46_003030"
     CDS             complement(<1..334)
                     /locus_tag="QBF46_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675184.1"
                     /translation="MINAVEYRLPLGNISPENLDALSELIVKHSDKFNNYVLSSISDD
                     DIRYSYDYYNFEITEIDEYGFHFIAPYSYYEGCVDNNFSGEVEGYAEYEIIDNELVFS
                     LEELPWDVK"
     gene            complement(520..1272)
                     /locus_tag="QBF46_003031"
     CDS             complement(520..1272)
                     /locus_tag="QBF46_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDQ6675185.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTR"
     gene            complement(1286..2275)
                     /locus_tag="QBF46_003032"
     CDS             complement(1286..2275)
                     /locus_tag="QBF46_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDQ6675186.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPDVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDKQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKVK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(2283..3080)
                     /locus_tag="QBF46_003033"
     CDS             complement(2283..3080)
                     /locus_tag="QBF46_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDQ6675187.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(3100..3489)
                     /locus_tag="QBF46_003034"
     CDS             complement(3100..3489)
                     /locus_tag="QBF46_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDQ6675188.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(3486..3812)
                     /locus_tag="QBF46_003035"
     CDS             complement(3486..3812)
                     /locus_tag="QBF46_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDQ6675189.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(3809..4462)
                     /locus_tag="QBF46_003036"
     CDS             complement(3809..4462)
                     /locus_tag="QBF46_003036"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDQ6675190.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPENADHIAFIRGRIGFELPVWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(4462..4956)
                     /locus_tag="QBF46_003037"
     CDS             complement(4462..4956)
                     /locus_tag="QBF46_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDQ6675191.1"
                     /translation="MIMSLLNEVQKYIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEAEPQPVRETRPVRNFYVGTNDPREILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQQERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(4953..5894)
                     /locus_tag="QBF46_003038"
     CDS             complement(4953..5894)
                     /locus_tag="QBF46_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6675192.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(5884..6063)
                     /locus_tag="QBF46_003039"
     CDS             complement(5884..6063)
                     /locus_tag="QBF46_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDQ6675193.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREDYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(6239..6796)
                     /gene="ymfL"
                     /locus_tag="QBF46_003040"
     CDS             complement(6239..6796)
                     /gene="ymfL"
                     /locus_tag="QBF46_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HDQ6675194.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(6840..7040)
                     /locus_tag="QBF46_003041"
     CDS             complement(6840..7040)
                     /locus_tag="QBF46_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6675195.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            7131..7805
                     /locus_tag="QBF46_003042"
     CDS             7131..7805
                     /locus_tag="QBF46_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HDQ6675196.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            8295..8891
                     /locus_tag="QBF46_003043"
     CDS             8295..8891
                     /locus_tag="QBF46_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675197.1"
                     /translation="MDWHDLISKVIIGLVTGSIAAYLTARYALGRYYKEKWWDKRIDL
                     YTQLVDCVYLLKNTADYWYKHELHHASQFPTYFSYKSKEENEILSKSFSNELEKLSRI
                     GELSSLFLTEECGDKIRSFIENEENLFIQWEFDSIELIDAHKSRLDKLKVLLDDIISE
                     AKRELKVNKKHNSLKNIKWSITMPDQKNDEPINYYDNH"
     gene            9314..9850
                     /locus_tag="QBF46_003044"
     CDS             9314..9850
                     /locus_tag="QBF46_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HDQ6675198.1"
                     /translation="MSFIQTLTGKKFNYINSTIDDIYIEDIAGALSNICRFAGHLPDF
                     YSVAQHSVLCSQLVAPEFAFEALMHDATEAYCQDIPAQLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPLLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            9841..10203
                     /locus_tag="QBF46_003045"
     CDS             9841..10203
                     /locus_tag="QBF46_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675199.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     LENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWNTWGQKETGDWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            10203..10508
                     /locus_tag="QBF46_003046"
     CDS             10203..10508
                     /locus_tag="QBF46_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675200.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     RESYELFMEKRFGGLVDSRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTISQQEVK"
     gene            10735..11898
                     /locus_tag="QBF46_003047"
     CDS             10735..11898
                     /locus_tag="QBF46_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6675201.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANIAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKEGTNDV"
     gene            complement(11913..11988)
                     /locus_tag="QBF46_003048"
     tRNA            complement(11913..11988)
                     /locus_tag="QBF46_003048"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11953..11955),aa:Thr,seq:cgt)
     gene            complement(12103..13356)
                     /gene="proA"
                     /locus_tag="QBF46_003049"
     CDS             complement(12103..13356)
                     /gene="proA"
                     /locus_tag="QBF46_003049"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDQ6675202.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPVGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MEESGVALHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(13368..14471)
                     /gene="proB"
                     /locus_tag="QBF46_003050"
     CDS             complement(13368..14471)
                     /gene="proB"
                     /locus_tag="QBF46_003050"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDQ6675203.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            14759..15814
                     /gene="phoE"
                     /locus_tag="QBF46_003051"
     CDS             14759..15814
                     /gene="phoE"
                     /locus_tag="QBF46_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDQ6675204.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(15853..16254)
                     /gene="crl"
                     /locus_tag="QBF46_003052"
     CDS             complement(15853..16254)
                     /gene="crl"
                     /locus_tag="QBF46_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDQ6675205.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(16312..17556)
                     /gene="frsA"
                     /locus_tag="QBF46_003053"
     CDS             complement(16312..17556)
                     /gene="frsA"
                     /locus_tag="QBF46_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDQ6675206.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(17648..18106)
                     /gene="gpt"
                     /locus_tag="QBF46_003054"
     CDS             complement(17648..18106)
                     /gene="gpt"
                     /locus_tag="QBF46_003054"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDQ6675207.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            18367..19824
                     /gene="pepD"
                     /locus_tag="QBF46_003055"
     CDS             18367..19824
                     /gene="pepD"
                     /locus_tag="QBF46_003055"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDQ6675208.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLHLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            complement(19881..20233)
                     /gene="prfH"
                     /locus_tag="QBF46_003056"
                     /pseudo
     CDS             complement(19881..20233)
                     /gene="prfH"
                     /locus_tag="QBF46_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(20229..20435)
                     /locus_tag="QBF46_003057"
                     /pseudo
     CDS             complement(20229..20435)
                     /locus_tag="QBF46_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
BASE COUNT         5363 a         5269 c         4824 g         5070 t
ORIGIN      
        1 attttacatc ccaaggtagt tcctcaagag agaagactaa ttcattatca atgatttcgt
       61 attctgcata accttcaacc tctcctgaga agttattgtc aacgcatcct tcataatagc
      121 tatatggtgc tataaagtga aacccatatt catctatctc tgtgatttca aaattgtagt
      181 aatcgtaaga atatcgaata tcatcatcag aaatagaact aagtacatag ttattaaatt
      241 tatcggaatg cttaacgata agctctgaca acgcgtcaag attctctgga gagatatttc
      301 ctaacggtaa tctgtactca acggcattaa tcatacaacc tcctttttaa ctgatatgag
      361 gttatataat gtgtttcagt acaagtaaat aaaaacccgc tcaacagcgg gttcgtgaaa
      421 attatcaacg gcagacatac aaagcccatc gttgagaaaa tcttatccat attttttgaa
      481 aaatgcaagc ttcatgtcgc catcttcggc gaaaatcact tatctcgtca cctttctcaa
      541 ttgcgtttca gcatatgctt cttcctgcca gcactttgtt actagtttat caattacgtc
      601 tgcatatcct ttgtaccact gataatcagt caggtctgga accagcttct ggacatgacg
      661 tcgtgccagt gtagttggta aacggctaaa ccggtttcca ttgcaacgcc cacaaatctt
      721 ataaacaggc gtgccatgaa gccgggtcct tttttcatcc aggacaatac ctttaccctt
      781 acaccctctg cacgctgtgc tgacttctcc cttaccatga caatgctgac atagttcctt
      841 cacccactct tccttgataa cagattcccc gcttctggag tgtttcacca cctcgcgcaa
      901 tacattatga aatccagtac cagcacaatg ctcacagcga gccttacttg ccgcagacct
      961 ggaataatca gcaaaggcaa aattcacaag gtaaggaatg atctgtagcc gggtttcttc
     1021 actcaattta ttcaatgtcg ggttatccag tgccatcgcg taattgagca gaccttcaat
     1081 cgcaaactga ggatcctgaa caccaacttt tgccaggaat aaggcaaacc caagcggtgc
     1141 tttcgactgc accatcccct gcgcagccat cacatccgta atcgttaaac caccagagcc
     1201 tgtcgccggt gcgtcatcgc tcagttttgg agatttcggg gagtaatatt tcggtaaggc
     1261 ttcaaggttc atgctcgttc tccacttacg ccagtacgcc tattgccagc gcacgatcga
     1321 taaaacgaaa tatcagctcc agctgggaac catacttctc ttcaaatgcc acggtatccg
     1381 catgcagctc gtcgtgatgc tttctgcaca aaggcaacac aaagaggtca tgcgcttttg
     1441 tacccattcc cccctgaccg tggcctatca ggtggtgggg atcatcagca ggctttccac
     1501 aacatgcaca cggctgtgtc ttaacccagc gtgtgtactt ttcattaacc cagcggcgac
     1561 gttttgggcg taacataaaa gactccggcg actccggatc cactttcagc gccagcacct
     1621 ttttcacctt atcctggatg atgctggtgg caggaaccga aggcacaagg tcactttccc
     1681 gggtgacaga cggcacaaca ggcttcggta atctcagtgc cttacgggct gcactttccg
     1741 gtaaggcatc cgccaggtca ttacgaatca gccaccagca cagttccggc attgtcacaa
     1801 cgtgactatc atcaaaaccg agatcccgac gcacaacaga caacacccag cgggcacagt
     1861 tatccgttgc cattgattcc agccgttccg tgaactgatc gcgcagctgt ttatcgcagt
     1921 gccagcacag acggattgcg cccggcgcgt gtcgcattgt tgtcatgttc tcgctgtgcc
     1981 agtcggaatg aggccactgg cagccttttt cacgaagtaa ccagctttca agacattcca
     2041 cgccaccagc acgacggatc actgcctcat tgcggaacac ggcccgaacg tcaggatcat
     2101 ccgccagcgg ttgtgatgcc gccggaacag caccactggc gaaagatgaa taacgttccg
     2161 gctcaggctc cagcaggaca cgcccctgca taaacagggg catcagctct gaacctggtc
     2221 tgaacaatac gatccccata cgcggggcaa tttcaggggt cagtagtgct ctcacggtca
     2281 cctcaatgaa cggtatcgag cagctttaac agctcaggga atcgggattc gaagaaatgc
     2341 ggctgcgtct cacgcggatt tgcgggactg gtgatattct tgccgaacat gcagcctttc
     2401 gctgtcagcg accagaattt tttgatgttg ttaatcgctg tacggctgta tcgttcgcgc
     2461 tgttcgacga tccccagctt cgccatctgg tgatatgcct gattagccgt aaggcggata
     2521 ccatactgct tcagcagtgc actcagtgac agcgtggggc ggcttgagcc atcaggcgcg
     2581 tcagcaggag catcaatggc atagcgcggt gccagattcg gtaagccaac agcctcctga
     2641 agtttctgac aggctccaag cactgaagag ttagacagat ttaactcccg gcgcataaag
     2701 tccagcagaa tcacaccagc ctgcatcttg tcagcagcct gtccggataa tttttccggt
     2761 gcgctggtta ccatgtcgaa agtacggatc accttcagat ggaatgacgg gctgatccac
     2821 attgcatagg catacaccag ttctttgcag acatacgtcc cctggttatt tccgccacga
     2881 ataacgttaa ctggctctat attgaccgag ttgcaaatct gcaactcgct tattaaacgc
     2941 tcagtttgct cattgcggag ccagaatgca ggcttatgct tatccagaga accggcagcc
     3001 ctgtgcagat cgttcaggct gtaacgccca taagcatcac gacgaacttc aataccatca
     3061 atgaccatca gattattcat acttcgtttc tcctcttaat caggcggctg cacccgccgt
     3121 tttctcgtac ttactgatag tgatctcgac cttcccttcc gggataaccg gtccccactc
     3181 caccagcatt cttttcacct gactgtcgtc ttcccacaca cccgcgtggg tcagggcgtc
     3241 aaacagcgcc ttgttatagt tgtccagatc gcggatccgg ttatccggag gaaacaacac
     3301 gatctccact gaagcaggtg ccgacgttgg ttttggcaga cgacgtaact gctcaactat
     3361 tgctgcacac gccgcgctct ggaatttgcg ccccgccgcg cttatcaggc tcttacctgc
     3421 aaacgcccct ttgttggggt gtcgccagta cgtgttcacg ctgggcggga aaggcagaat
     3481 cagcttcata ctttcagacc cctctcatgt aaccagtggg ctgcacgcag cctggcgttt
     3541 tcctcaccgg caagcagtga gcggataatc ccgaccgcct cgctgtcgtc gtccttcacc
     3601 gcggtatgaa gcgttatccc ccgggccaca ccacgcttta tcgtgatgac gccttttttc
     3661 tccagtgcgc gaagatgctc caccgctgca ttcactgaac gatatcccag catggttgcc
     3721 acctcctgat tggttggcgg aaagccacgc tctttctggt aagaaatcag catatccagc
     3781 acctgctgct ggcattgagt taacgtcgtc atgccgccat ctccctgacc agtttttccg
     3841 cctgctggcg aacctgcgcc agaaaggcct caccacatgc ctcaagttca tcgcgcccga
     3901 tgtagctgat tgccggtccc ttccaggtct tgtcgaaaac agcaatagca ccagcgaaga
     3961 aagcgcctgt cggtacctgc ttctcgtctt tcgggataaa ccagacaggc agttcaaaac
     4021 caatacgccc gcgaataaaa gcaatatgat ctgcattttc cggccaccac acttcgctgg
     4081 tggcagcttt gatcaggaaa acatagcgcc cgcctttatc acgcatggca ctggcatgct
     4141 tcatgatgta acgcatgccg gtgatgtatt gcccctcatg ctgactggcg cggctgtatg
     4201 ggggattacc aaaggcagca cctttaagct ccgcaagacg ttctgaccag tcatgcgcca
     4261 gcgcgttgtc ttccgccgtg taatacgcgg cacatttggc gttatcaccg tcagtgaaca
     4321 gatccagaac aaacgggcca aacagggtgt taattcccca gaaaatgttg tccggcgtgc
     4381 gccactgatc gcccacttcc ttcagttcat gggctggttt gttccgcagt tccaccagcg
     4441 cctggcaata tttattactc attaagcccc cacgtaattc cctgacagat accactcttc
     4501 acccgatgca gcgcgcttgc tgcttttccg taagcaccgc tcacgacgcg ccagaaaatt
     4561 gtttcgctct tgctgggagt ggctttcacg gaatgccgcc atccacaccg ttgcagcacg
     4621 acggtataag cccctggact ccagttcttc cgcctggcgg gtcaggcaca aaatctcccg
     4681 cgggtcgtta gtgccgacat agaaattgcg cacaggtctg gtttcacgaa ctggttgcgg
     4741 ttccgcctcc tgcgctctct cagtcaggcg cgggaaatgt ctgcgtgtat ccccttcaca
     4801 acggtgagcc acacgaccac tctgacgtaa cttgcttgct gactgcagaa cgcgctgccg
     4861 tgagtaacct gcaaaagcat ccgcaatgtc tccggaagta caccccggat gggcttcaat
     4921 gtatttctga acttcattca aaagactcat gatcaccccc tgaatcctgc cgggatctgg
     4981 ctgtagtcca cgttgtcgta actggctttg aagtacgggt cttcgcgttt ttcggtgtac
     5041 gtgctgacgg acggcgataa gcgcagggaa agctcatccc atttttcccg cagcttcgac
     5101 gggctgagca cgttacggca ccagaacgga tcgcggctga cgcggctgta catctcgcag
     5161 atttgtttgt gagtacgacc atcctgcaca cacatcaggc gaatttcgtt tgcccaggct
     5221 gtccagttcg gttctttggg acgaaccacc tcgccgtcac attcggcggc ctgctcgtac
     5281 agggcgatga tttttttcca gagccactgt gcgcaggtca aatcatcctg cgttccccac
     5341 tggcgctttt tagggctgaa tacaaccgca tcaggatggc gagttaaaaa ctcctgttca
     5401 gccgtctgcg tgtccggttg cgaagcgtcc ggacgagaag tttttttatc tgacggatca
     5461 tgttttgatt ttactgacgg atccccgcca gattctgacg ggtgaaaacc cgcttttttg
     5521 ccagatttcg acgcatcaaa ttttgacggg tcagattttg atgcgtcaga ttttgacggg
     5581 tcagaatctg acagttgaga aaatgccgct gcctgaagct tcgcaacgtt aagctgataa
     5641 acattcgacg cattgcggtt accctggcga cgcgccttac gcgttaacca gccttctgct
     5701 tccagccgtg cgatagccgt tctgacggta ctcatccccg cgccaatctg acgggcaatg
     5761 gtttcaattg atggccagca cacaccttcg tcattactga aatcagccag gcgggccata
     5821 attgccacgc tggataattt catgcctgac gcagcgcaac catcccatac atagccggtt
     5881 aatttagtgc tcatgaccga cctctatttc cctgaattta cgacgaaact gttcgagcgg
     5941 gctgaagcac tcatgctcat agtcttcgcg gaggtagata acccgttgtg tttccggctc
     6001 ccaacgaatg actctgacgg gtactccgta gtgatctttg aaccagcggt taacttgtcg
     6061 caaaggactg tctccttctg ccggttgaaa tcacccacag cccactctgc aaagctgtgg
     6121 gttacaattt ccctgtcacc tggtacattc actgcatagc aatattccac cttcgctttt
     6181 ccacccggta caggaagcgc aatcagttgc gagcgacggt agtgtgttgt taaactgttc
     6241 atgcgttagt ttctccacaa ccagaagcaa tcgacgccac gacgcccgga gctgcacact
     6301 cgcgggcgtc attactttct gaaatgcaaa aaattttgta gacaagtgct gcatgctcct
     6361 gcagcttcga aattgagaga tacagctcgt cgttaattgc tgtcttctca tgcggttcca
     6421 ctacaccgtc ttcgattgct gaacgaatct gttttgaata actgccgatc tgttcaatga
     6481 cttccagcag acgctggtta atatcggcgt tgtccacatc ctcgacgtca ggaagagaca
     6541 caaagacgcc atttgcagac tgcgccacag catcagcaat gaagtgagtg ccaccagcac
     6601 gctgtaaaac cattgcccat cccagcggga aaatctgatc gccatctgca cgaaggcggt
     6661 tgaataaagc gttttctgtt acatcgagcc agtcagccgc ttcagcgtaa ccacccggca
     6721 acgccgcgat agtttttctg acagctttca cgtaccactc aggctgtttt tctattttcc
     6781 agtgatgctt acccacgatt agcctcatcg ttctgtggtt aaaaattgaa agtgttctgc
     6841 taatctttcg gatagatatc cggtcttaag tcagatttcg taattgcacc tgacgtgcat
     6901 tgctcaagtt ttttagccag cacaaaactg gcttttttat agccattgaa aaccagccgt
     6961 aagtagccag gtgttgagcc aacttttccg gccaactcgc cctgctgttc tttggttaaa
     7021 gagtcccaat acgctttcat acaatatgta cctccgatgt acatattaca tgattgaaat
     7081 gaaccttcaa gatacttgta ccttaacggt acaagggttt taatttcgtt atgaaaacaa
     7141 tccatgacat ccggcggtct aacgccagaa aactgagaga tggtgttggc gggaattctt
     7201 cctttgccac tatgattgat cgcgagccaa cccagaccag caggtttatg ggagatggtg
     7261 ctactaaaaa tatcggtgac agcatggcac gacacatcga aaaatgtttc gacctgcctg
     7321 tcggatggct cgatcaagaa caccagacaa cgaacatcac aaaaaaacct gatgtttcaa
     7381 tcactaataa acaaatcaca ttagtccctg tcatatcatg ggtacaggcc ggagcatgga
     7441 aagaagttgg atattctgag gttgatttga gcacagcaga aacgtatccc tgccctgtcc
     7501 cctgtgggga aatgacttat atcttgcggg tgataggtga ttcaatgatt gatgagtacc
     7561 gcccgggaga catgattttt gtcgatcctg aagtacctgc ctgccacggt gacgacgtta
     7621 ttgcattgat gcacgataca ggtgaaacca ccttcaaaag gttgatagaa gatgggacac
     7681 agcgttatct caaagcgtta aacccaaact ggcctgagcc ttacattaag atcaacggta
     7741 attgctctat aattggtaca gtgattttct caggaaaacc aagaagatac aaaatcaaag
     7801 cctaatcaat gtttatgaac ctgcttcggc aggttttttt atacttgaca atgtaccttt
     7861 gagatacata atgtacccaa gagaaacaac aaacaggcag gacgtccacg aagtagccgc
     7921 ctggggcata tgaagtccaa gatgattcgt tagcaacaaa acgcccatag gacgcttcgc
     7981 tctttaacaa tttgaatatt ttcagcgtca atgatctgtt tagctcccta caagtaaatg
     8041 tagcgtagca ccatgataaa tcctgctagt gtttagttaa aaatcgcgcg agagcaagaa
     8101 cgacgtgagg gcaggcaata ttgatagtgt tattgctatt agtttatcaa aaatatttag
     8161 acgacccaga tgaatatttt taatacatac tccccctaat ttaacaggag taaatgttga
     8221 tgaataacga gacatctata ctcaacaatt gtatgatatg aacaattata taacataatg
     8281 taggaaaaat aaaaatggat tggcatgacc ttatatcaaa ggtaattatc ggacttgtta
     8341 caggctcaat cgcagcttac ttaactgcgc gatatgcact cggccgttat tataaagaaa
     8401 aatggtggga caaaagaata gatttgtata cgcaattagt tgactgtgtt tatcttctaa
     8461 aaaacacagc tgactattgg tataaacatg agttacatca cgcttcacaa ttcccaacct
     8521 actttagtta caaatcaaaa gaagaaaatg aaatccttag caaatctttc tctaatgagc
     8581 ttgaaaaact cagtagaata ggtgagctat cttcattatt cttaacagaa gagtgtgggg
     8641 ataaaatccg ttcattcatt gaaaatgaag aaaatctatt tatacaatgg gagtttgata
     8701 gtatagagtt gatagatgcc cacaagtcaa gacttgataa attaaaagta ttactagacg
     8761 atattatctc tgaagcaaag agagagctta aggtcaataa aaaacataac agcctaaaga
     8821 atataaagtg gtccataact atgccagacc aaaaaaatga tgagccaatt aattattacg
     8881 acaatcatta aaacacttta gaacccgctg ggcgggtttt attcaaatat actttgcaca
     8941 tcacaaataa aactataacg tccttcttct tttcctaata tcttatatct acttatttat
     9001 aagcggctta cagatcttca acagataaac ctaatgaagg cgacaatgtt ctgatgaata
     9061 gccacctttt tactcaactg aaagcgcgtt ctgtcccttg tcattaagtg ccagttcgtt
     9121 aagtccaaaa cctccggaac gcgctttcaa ttgagtgggg aaaataactg gcgatggcag
     9181 ccgcccgttg cactaagtgc ctctggatgg ggcatttact gaaacgaaac caaaactttt
     9241 aatcgcctta tggcgaggga ttcgtgcaac caaaaatcgt gcggtgcagc gcaccaatta
     9301 aggagaacta acgatgagtt ttatccagac tcttaccggt aagaagttca attatatcaa
     9361 ctccaccatt gacgatattt atatcgagga tatcgccggt gcgctttcaa atatctgccg
     9421 cttcgcggga cacttgccgg atttttacag cgtggcgcag cattccgttc tgtgcagtca
     9481 acttgtggcg ccagagttcg cctttgaagc gttaatgcat gatgcaacag aagcgtattg
     9541 tcaggacatc cccgcgcaac tgaaacgcct tcttcctgac tataaacgga tggaagaaaa
     9601 aatagacgcc gtaatccgtg agaaatacgg gttacccccg gttatgagca cgcccgtgaa
     9661 atatgccgat ctcatcatgc tggcaaccga acgccgcgat ctcgggcttg atgatggctc
     9721 tttctggcct ctactggaag gtatcccagc aacagagatg ttcaaagtta ttccactgtc
     9781 gccaggccat gcctacggga tgtttatgga acgttttaac gagttatcgg agttacgcaa
     9841 atgcgcatga atgttttcga aatggaaggg tttcttcgcg ggaaatgtgt accgcgagat
     9901 ctgaaagtga atgaaacgga tgctgaatac ctggtgcgta aattcgatgc gcttgaagct
     9961 aaatgtgcgg cactggaaaa caaagtaata ccagtgtcaa ctgaactgcc accagcaaat
    10021 gaaagtgttt tgttattcga tgctaacgga gaaggctggc taattggctg gcgttctctc
    10081 tggaacacct ggggacaaaa agaaaccgga gactggcagt ggacatttca ggtcggggac
    10141 cttgaaaacg tcaatatcac tcactgggca gtaatgccaa aagcaccgga ggctggagca
    10201 taatgaccac ttttaccgac aaagaactga ttaaagaaat caaagagcgt atcagcagcc
    10261 ttgacgtgcg agacgatatt gagcgccgtg cttatgaaat cgcactccta tctctggaag
    10321 tagagccaga tgaacgcgaa tcctatgaat tatttatgga aaaacgtttc ggtggcttag
    10381 tagatagtcg gagagcaaaa aacggcgata acgaatacat ggcatgggat atgactctcg
    10441 gttggatcgt ctggcaacaa cgagctggca ttcatttttc aacaatatca cagcaagagg
    10501 tgaaataatg gaaccataca gcctcacgct agatgaggcc tgtcattttc tcaagatatc
    10561 cagaccgact gccattaact ggatacgcac agggcgtctg caggcaacac gcaaagatcc
    10621 cactaagaat aaatctcctt acctcacaac acgacaagcc tgcattgcgg cgcttcagtc
    10681 tccgctgcat actgtccagg tgagcgcggg tgatggcata acagaggaaa gaaaatgtca
    10741 ctcttccgca gaggtgaaat atggtacgcc agtttcacat tgccgaacgg taaaagattt
    10801 aaacagtctc ttggaacaaa ggacaaaagg caggcgacag aactccatga caagctaaag
    10861 gctgaagcat ggcgggtcag caaacttggt gaaattcctg atataacgtt cgaggaagcg
    10921 tgtgtcaggt ggcttgaaga gaaagcacat aaaaaatcac tggacgatga caaaagccgg
    10981 atcggattct ggcttcaaca tttcgcagga atgcaactaa gagacattac tgaatcaaaa
    11041 atttattcag caatgcagaa aatgacgaac cggcgtcatg aggaaaactg gaaactcagg
    11101 gcagaagcat gcagaaaaaa agggaaacct gttccagaat acacgccaaa accagcgtcc
    11161 gttgcaacga aggctacgca tctttcattt ataaaggccc tgctaagagc cgcagagcgt
    11221 gaatggaaaa tgctggataa ggcaccaatt attaaagtgc ctcaaccaaa gaataaacgg
    11281 atccgctggc tggagcctca tgaagcacaa aggctgattg atgaatgtcc ggagccatta
    11341 aagtctgttg ttgaatttgc acttgcaaca ggcttaagac gctcgaacat catcaacctt
    11401 gaatggcaac aaatagacat gcagcgccgg gtggcatgga taaacccgga agagagtaaa
    11461 tcaaaccgcg caattggcgt tgcgctgaat gatactgcat gtcgcgtatt gaaaaaacaa
    11521 atcgggaatc atcaccgttg ggtatttgtg tataaggaaa gctgtaccaa accagacgga
    11581 acgaaagcgc caacagtcag gaagatgcgg tatgacgcaa acatagcctg gaaagcggcg
    11641 ctgagacgag caggtattga tgatttcaga tttcacgact tgagacacac ctgggcaagt
    11701 tggctggttc aagccggagt cccgttatca gtgttacagg aaatgggagg ctgggagtct
    11761 atcgaaatgg ttcgtcgata tgctcacctc gcacctaatc accttaccga acacgcacgg
    11821 caaatagact cgattctgaa cccatcggtc ccaaatttgt cccagtcaag aaataaggaa
    11881 ggtactaatg atgtgtaact tattgattta aatggtgccg ataataggag tcgaacctac
    11941 gaccttcgca ttacgaatgc gctgctctac caactgagct atatcggccc tgaaaggaca
    12001 tgttcacgaa cgtgaatcac ggtggacaag gttaaaacta accgggcgat gcgtcaatgg
    12061 ccttgtgaat caaatggcta cttttgcatc acccggtttt atttacgcac gaatggtgta
    12121 atcaccaata ccgatccact tgtaagtggt cagtgcttcc agccccattg ggccacgcgc
    12181 gtggagtttt tgtgtgctta ccgccacttc cgcacccagt ccaaactggc cgccgtcggt
    12241 aaaacgcgta gaggcgttaa cgtaaacagc ggacgaatcc acttcgttaa caaaacgctg
    12301 ggcgttgcgc atatcgcggg tcaggatcgc atcggagtgt tgtgtgccgt gttcacgaat
    12361 atgggcgatg gcatcgtcaa gatcactgac gattttgacg ttcaaatcta atgacagaaa
    12421 ctcatcgtca tactcttccg ctttaacagc caccaccttc gcggggcctg tctgcaactg
    12481 cgccagcgca gctgcatctg cgtgtaatgc cacgccgctt tcctccattt gtttgcttaa
    12541 tgcgggcagg aagctatcgg cgatgttttt attcaccagc aacgtttcta ccgtattaca
    12601 tgtgctcgga cgctgagttt tcgcgttgac gatcactttt aatgcttcag caatctctac
    12661 actttcatca acataaatat ggcatacgcc tataccacct gtgatcaccg ggatcgtcga
    12721 ctgttcgcgg cacagtttat gcaaaccagc gccaccacgc gggatcagca tgtcgatgta
    12781 tttatccata cgcagcattt cactgaccag cgcacggtca ggattatcaa tcgcctgcac
    12841 ggcacccacc ggtaagccac aggatttcag ggcgtcctga atcaccgcca ccgttgccgc
    12901 gttagtgcga caggtttctt taccgccacg cagaatcact gcgttaccgg ttttcaggca
    12961 cagcgaagcg acatcaaccg tcacgttcgg gcgcgcttca taaatcacgc caataacccc
    13021 cagcggtacg cgacgacgct caagacgcag gccgctgtcc agtacgctgc catcgattac
    13081 ctgccccacc ggatcggcga ggttacacac ctggcgcaca tcatcggcaa tgcctttcag
    13141 ccgtgcgggc gtcagtgcca gacggtcaag catcgcttcg ccaaggccat tggcacgcgc
    13201 gtcagcaaca tcctgggcgt tagcgttgag gatgatttcg ctttgtgctt ccagttcatc
    13261 ggcgattttt tccagcacgc gatttttttc gcggctggag agttgcgcta atttatacga
    13321 ggcttgcttc gcggcaatgc ccatttgttc cagcatcagc ctgctcctta acgggtaatc
    13381 atgtcatcac ggtgaacggc aaccgggccg tattcatatc ccagtattgc atcaatttct
    13441 tgcgagtggt gcccggcaat acggcgtaat gcatcgctgt tgtaacgact gacgccgtgg
    13501 gcgatatcgc gaccttcgag gttgcaaatg cggatgactt caccacgcga gaaattgcca
    13561 gtcacgcttt taatgccttt cggcaacagg gagctgccgc gttcaagaat ggcggcggtt
    13621 gccccttcat ctaccgtgat ttcacccgcc ggcggcgcac cgaaaatcca gcgtttacgg
    13681 ttttcaagcg gagtcgcctg ggcatggaac agcgtaccga cggaaatgcc ttccatcaca
    13741 tcaccaataa cgcccggctt gctgcccgcg gcaataatgg tgtcgatacc cgcacggcaa
    13801 gccacgtcag cggcctgcaa tttggtactc atgccgccag ttccgaggcc tgaaacgctg
    13861 tcaccggcaa tcgcgcgcag tgcgtcatca atgccgtaaa catctttaat cagttctgcc
    13921 tgcggattgc tgcgcggatc agcggtatac aaaccttttt gatcggtcag cagcaacagt
    13981 ttatcggcac ccgccagaat cgccgccagc gcagaaaggt tatcgttatc gccgacctta
    14041 atctctgccg tagcgacagc atcgttctca ttgattaccg gaacgatatt gttatcgagc
    14101 aacgcacgca gggtgtcgcg ggcgttcagg aagcgttcac ggtcttccat atcagcacgg
    14161 gtcagcagca tctgcccgac gtgaatgcca taaatcgaaa acagctgttc ccacagttga
    14221 atcagtcgac tctgccctac cgccgccagc aattgtttcg aggcgatagt cgctggcagt
    14281 tccgggtaac ccaggtgctc acgtccggcg gcaatcgcgc ccgacgtcac aataacaatc
    14341 cgatgcccgg cggcatgtaa ctgcgcgcac tggcgaacaa gttcaacgat atgggcacgg
    14401 ttcagacggc gcgatccgcc tgttagcaca ctggtgccga gttttaccac cagcgtctgg
    14461 ctgtcactca tgattctctg ccattcaatt ttaggaaaaa tgatatcaaa cgaacgtttt
    14521 agcaggactg tcgtcggttg ccaaccatct gcgagcaaag catggcgttt tgttgcgcgg
    14581 gatcagcaag cctagcggca gttgtttacg cttttattac agatttaata aattaccaca
    14641 ttttaagaat attattaatc tgtaatatat ctttaacaat ctcaggttaa aaactttcct
    14701 gttttcaacg ggactctccc gctgaatatt cgcgcgttaa ttaaaatcag gaatgaaaat
    14761 gaaaaagagc actctggcat tagtggtgat gggcattgtg gcatctgcat ccgtacaggc
    14821 cgcagaaata tataacaaag acggtaataa actggatgtc tatggcaaag ttaaagccat
    14881 gcattatatg agtgataacg acagtaaaga tggcgaccag agttatatcc gttttggttt
    14941 taaaggcgaa acacaaatta acgatcaact gactggttat ggtcgttggg aagcagaatt
    15001 tgccggtaat aaagcagaga gtgatactgc acagcaaaaa acgcgtctcg cttttgccgg
    15061 gttgaaatat aaagatttgg gttctttcga ttatggtcgt aacctgggcg cgttgtatga
    15121 cgtggaagcc tggaccgata tgttcccgga atttggtggc gactcctcgg cgcagaccga
    15181 caactttatg accaaacgcg ccagcggtct ggcgacgtat cggaacaccg acttcttcgg
    15241 cgttatcgat ggcctgaact taaccctgca atatcaaggg aaaaacgaaa accgcgacgt
    15301 taaaaagcaa aacggcgatg gcttcggcac gtcattgaca tatgactttg gcggcagcga
    15361 tttcgccatt agtggggcct ataccaactc agatcgcacc aacgagcaga acctgcaaag
    15421 ccgtggcaca ggcaagcgag cagaagcatg ggcaacaggt ctgaaatacg atgccaataa
    15481 tatttatctg gcaacttttt attctgaaac acgcaaaatg acgccaataa ctggcggctt
    15541 tgccaataag acacagaact ttgaagcggt cgctcaatac cagtttgact ttggtctgcg
    15601 tccatcgctg ggttatgtct tatcgaaagg gaaagatatt gaaggtatcg gtgatgaaga
    15661 tctggtcaat tatatcgatg tcggtgctac atattatttc aacaaaaata tgtcagcgtt
    15721 tgtagattat aaaatcaacc aactggatag cgataacaaa ttgaatatta ataatgatga
    15781 tattgtcgcg gttggcatga cctatcagtt ttaatgatta ttgccggatg tgatgcatcc
    15841 ggcacatttc actcacgccg ttaacttcac cggctcgtca cgaaaatcat ccgccggttc
    15901 cagcttcaga ttcagcgtcg tcagcagttc acgcagcttc tcgtgaaact cacgcagggt
    15961 gtgctccagt cgttcaacca cttcagtatc ttttaccgga acactcttcc agtcgcctgc
    16021 tttatcgaac agaccaaact ggtaactgta ggtaaaacgg gattcctgcg cttcaagctc
    16081 catccaccag ccccagaact cacgcacttc cggtgccggt ttcacgttga cgcatacagc
    16141 cagacaatcg aaaaagaatc gattatcttc gcacttacct tcacgaatat acgggcctag
    16201 tgcggtaaat tttttgatca atctgctctt cgggtgtcca ctcggtaacg tcattgctat
    16261 ctcctgttgt gatgcaactg ttttaccaaa ttggcaaaat ttagcaaatt tttaacacaa
    16321 acgtttttcg atccagtctg tgatttcctg aagacctttg tcaaaattcc gatacaccgg
    16381 gttaaatggg atctctaata atttaccgtc agcagatgat gaggtgatta agcgtgagtc
    16441 ctcttctggg ctgaacggat cgttcttcca gtagcctgat aacattggcg ttgggcagcg
    16501 acgtcccagc aatccttgca cttttaatga atagcgattc agctccacgc gcaacgcttc
    16561 gtcggaagca tcatgcatcc ccaaacgact cgccagaacg tcaagataca tttccggcac
    16621 ctgttgctgg cacttaaaat cactcaacag ggtatgaact accggaccaa gacaggcaac
    16681 cgctttcaga cgcggcgatt caaggtatgc cagacgcacg gcaacgttag cgccgaaacg
    16741 gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt acgttaggca gcgcctttaa
    16801 gacgtgctga tgcaacaggc tggagtcctg ggtgagcttc cattttgatg aaaagcccac
    16861 cgacggcata tcaatagtca gcatcgcaat gccgcgcggc gcaaaataac gttcatacag
    16921 gctgtaatag tccgtctgca tcgcatccag accaccacac attaataccg tcgggaacgg
    16981 gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa
    17041 ctccatctgc cgcatcgtgc ccggtagacg ctgagcggcc tcttcatagg cgcggtttga
    17101 caaagcctgc gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg cggcaatgtt
    17161 gtacaacgta gccgcatgca gccagtgacg accactgagt tgcggatctt cttccgcaca
    17221 ggctttttgt tgccacacca tcgcctgggt ggcccactca taaatccagt tgccgccacg
    17281 gtagccaatc accgtatcgt ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat
    17341 acgtgcctgg acgtcgagga tttcgcgtgg atcaatgccg cgccagatcc acatcagacg
    17401 gttaatcatg cgataccagt gagggatggt tttaccatca agggccgact gcacaggcgg
    17461 ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg
    17521 tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca gaatacttac
    17581 tggaaactat tgtaacccgc ctgaagttaa aaagaacaac gcccggcagt accaggcgtt
    17641 gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt
    17701 cgatccaggt atcttgcggg atatcaacaa cataatcatc aaccagcgga cgaccagccg
    17761 gttttgcaaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag
    17821 taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc
    17881 gtttcagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat
    17941 cgacatgacg aatacccagt tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc
    18001 ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg
    18061 catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag
    18121 cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga
    18181 gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc
    18241 tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgttga
    18301 caacattttc tgctaaccct gtgacctgca atactgtttt gcgggtgatc gacaaggaga
    18361 cttaacgtgt ctgaactgtc tcaattatct ccacagccgc tgtgggatat ttttgccaaa
    18421 atctgttcta ttcctcaccc gtcctatcat gaagagcaac tcgctgaata cattgttggt
    18481 tgggcaaaag agaaaggttt ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt
    18541 aaacctgcta ccgcaggtat ggaaaatcgt aaaccggtcg tcttgcaggc ccacctcgat
    18601 atggtgccgc agaaaaataa cgacaccgtg catgacttca cgaaagatcc tatccagcct
    18661 tatattgatg gcgaatgggt taaagcgcgc ggcaccacgc tgggtgcaga taacggcatt
    18721 ggtatggcgt ctgcgctggc ggttctggct gacgaaaacg tggttcacgg cccgctggaa
    18781 gtgctgctga ccatgaccga agaagccggt atggacggtg cgttcggctt acagagcaac
    18841 tggttgcagg ctgatattct gattaacacc gactccgaag aagaaggtga aatctacatg
    18901 ggttgcgcgg ggggtatcga cttcacctcc aacctgcatt tggatcgtga agcggttcca
    18961 gctggctttg aaaccttcaa gttaacctta aaaggtctaa aaggcggtca ctccggcggt
    19021 gaaatccacg ttggcctggg taatgccaac aaactgctgg tgcgcttcct ggcgggtcat
    19081 gcggaagagc tggacctgca ccttatcgat ttcaacggtg gcacactgcg taacgccatc
    19141 ccgcgtgaag cctttgcgac cattgctgtc gcagctgata aagtcgacgc cctgaaatct
    19201 ctggtgaata cctatcagga catcctgaaa aacgagctgg cagagaaaga gaagaatctg
    19261 gccttgttgc tggactctgt agcgaacgat aaagctgctc tgattgcgaa atctcgcgat
    19321 acctttattc gtctgctgaa cgccaccccg aacggtgtga tccgcaattc cgacgtggca
    19381 aaaggtgtgg tcgaaacctc cctgaacgtc ggtgtggtga ccatgactga caataacgta
    19441 gaaattcact gcctgatccg ttcactgatc gacagcggta aagactacgt ggtgagcatg
    19501 ctggattcgc tgggtaaact ggctggcgcg aaaaccgaag cgaaaggcgc atatcctggc
    19561 tggcagccgg acgctaattc tccggtgatg catctggtac gtgaaaccta tcagcgcctg
    19621 ttcaacaaga cgccgaacat ccagattatc cacgcgggcc tggaatgtgg tctgtttaaa
    19681 aaaccgtatc cggaaatgga catggtttct atcgggccaa ctatcaccgg tccacactct
    19741 ccggatgagc aagttcacat caaaagcgta ggtcattact ggacactgct gactgaactg
    19801 ctgaaagaaa ttccggcgaa gtaattattt gatttgctgc cggatggcgt ttaatcgcct
    19861 tccggcagtt tcatccttca ttatccttcg ataaaagcca tccctgtaaa tgtccgtcgc
    19921 gggttgccac gttcaatctg gtgatggaac attcgccgct gcgatttcag cgccgcgcta
    19981 ttttcctgtt gctgctgctc cagcttccag gcaatcagca atcgcgccag ccgtttgtta
    20041 gcatgcccag aaaccagttt ttgcgcccat gatgagaccg atacggactc ggacaaatcc
    20101 actgaatagt gccgcaccag ctttcgctta atgcccatgc gttatcgcca tccagagaaa
    20161 ccagcgccga acgcagcgtg tcagagtagc ggcccgtttc tgtttccagc accgttaccg
    20221 cgacgtcttg ccggataaaa acgaccaaca gagaagaacg ctgcgccaat cggatagccg
    20281 cgtccgggat gtaaatctgg catccccact acgcgctgca tgttgggtaa atttgccgtg
    20341 gtgtgtaatt gttggatcgc taaactctcg atccacaggt catcagatgc gatggtaaat
    20401 accgcatcgg ataagggacg aatatatttg cccatgtacc attccattta tactcgtcat
    20461 acttcaagtt gcatgtactg cgactgcgtt cgttcacccc agtcacttac tttagtaagc
    20521 tcctgg
//
LOCUS       DAOFQD010000080        20295 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.080, whole genome shotgun sequence.
ACCESSION   DAOFQD010000080 DAOFQD010000000
VERSION     DAOFQD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.080"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            174..1151
                     /locus_tag="QBF46_003058"
     CDS             174..1151
                     /locus_tag="QBF46_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDQ6675209.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1221..1703)
                     /locus_tag="QBF46_003059"
     CDS             complement(1221..1703)
                     /locus_tag="QBF46_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDQ6675210.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1938..4520
                     /gene="leuS"
                     /locus_tag="QBF46_003060"
     CDS             1938..4520
                     /gene="leuS"
                     /locus_tag="QBF46_003060"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDQ6675211.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4535..5116
                     /gene="lptE"
                     /locus_tag="QBF46_003061"
     CDS             4535..5116
                     /gene="lptE"
                     /locus_tag="QBF46_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDQ6675212.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYHMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5116..6147
                     /gene="holA"
                     /locus_tag="QBF46_003062"
     CDS             5116..6147
                     /gene="holA"
                     /locus_tag="QBF46_003062"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDQ6675213.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENSAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6149..6790
                     /gene="nadD"
                     /locus_tag="QBF46_003063"
     CDS             6149..6790
                     /gene="nadD"
                     /locus_tag="QBF46_003063"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDQ6675214.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6814..7424
                     /locus_tag="QBF46_003064"
                     /pseudo
     CDS             6814..7424
                     /locus_tag="QBF46_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            7684..8001
                     /gene="rsfS"
                     /locus_tag="QBF46_003065"
                     /gene_synonym="rsfA"
     CDS             7684..8001
                     /gene="rsfS"
                     /locus_tag="QBF46_003065"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDQ6675215.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            8005..8472
                     /gene="rlmH"
                     /locus_tag="QBF46_003066"
     CDS             8005..8472
                     /gene="rlmH"
                     /locus_tag="QBF46_003066"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDQ6675216.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8503..10404
                     /gene="mrdA"
                     /locus_tag="QBF46_003067"
     CDS             8503..10404
                     /gene="mrdA"
                     /locus_tag="QBF46_003067"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDQ6675217.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10407..11519
                     /gene="mrdB"
                     /locus_tag="QBF46_003068"
                     /gene_synonym="rodA"
     CDS             10407..11519
                     /gene="mrdB"
                     /locus_tag="QBF46_003068"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDQ6675218.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11530..12618
                     /gene="rlpA"
                     /locus_tag="QBF46_003069"
     CDS             11530..12618
                     /gene="rlpA"
                     /locus_tag="QBF46_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDQ6675219.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12758..13969
                     /gene="dacA"
                     /locus_tag="QBF46_003070"
     CDS             12758..13969
                     /gene="dacA"
                     /locus_tag="QBF46_003070"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDQ6675220.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            14080..14343
                     /locus_tag="QBF46_003071"
     CDS             14080..14343
                     /locus_tag="QBF46_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDQ6675221.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14444..15085
                     /gene="lipB"
                     /locus_tag="QBF46_003072"
     CDS             14444..15085
                     /gene="lipB"
                     /locus_tag="QBF46_003072"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDQ6675222.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLKNPDFEYITA"
     gene            15344..16297
                     /locus_tag="QBF46_003073"
     CDS             15344..16297
                     /locus_tag="QBF46_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDQ6675223.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALFLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16506..17471
                     /gene="lipA"
                     /locus_tag="QBF46_003074"
     CDS             16506..17471
                     /gene="lipA"
                     /locus_tag="QBF46_003074"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDQ6675224.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17572..17775)
                     /gene="tatE"
                     /locus_tag="QBF46_003075"
     CDS             complement(17572..17775)
                     /gene="tatE"
                     /locus_tag="QBF46_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDQ6675225.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17904..18692)
                     /locus_tag="QBF46_003076"
     CDS             complement(17904..18692)
                     /locus_tag="QBF46_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDQ6675226.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18785..19168
                     /gene="crcB"
                     /locus_tag="QBF46_003077"
     CDS             18785..19168
                     /gene="crcB"
                     /locus_tag="QBF46_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDQ6675227.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19222..19431)
                     /gene="cspE"
                     /locus_tag="QBF46_003078"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19222..19431)
                     /gene="cspE"
                     /locus_tag="QBF46_003078"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDQ6675228.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19606..20166)
                     /gene="pagP"
                     /locus_tag="QBF46_003079"
     CDS             complement(19606..20166)
                     /gene="pagP"
                     /locus_tag="QBF46_003079"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDQ6675229.1"
                     /translation="MNVSKYVAIFSFFFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5136 a         5122 c         5075 g         4962 t
ORIGIN      
        1 ccatgtccat ataatttatc cttaaatatt cattaaatga atattagccg cattgcgagg
       61 ctcgtttatt tatacttttc tgacgcgaca taatacgcat aaaataaggc ttacgcaaaa
      121 atggaacaca taaaaatata atatgtttta tctgcaacca taaggaaatg ataatgattt
      181 tcacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt gctgaaaaag
      241 gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc gcaattgatt
      301 ctccggacga cgaaaaagcc ttttactggc tgaagctggc cgctgagcaa ggtcattgtg
      361 aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat aaagataatg
      421 agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc gcttccaacg
      481 cccttggctg gacactggat cgtggagaag cccccaatta taaagaagcg gttgtctggt
      541 atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg tggatgtaca
      601 gaaatggcaa cggagtcgca aaagactatg cgctggcatt tttctggtac aaacaagctg
      661 cattacaagg ccatagtgac gcgcaaaaca atctggccga tctttatgaa gacggaaaag
      721 gcgttgctca aaacaagaca ctcgccgcat tctggtattt gaaaagcgca cagcagggta
      781 atcggcacgc ccagtttcaa attgcatggg attataacgc tggcgaaggg gtggaccagg
      841 actataagca agcgatgtac tggtatctga aggctgccgc tcaggaaagc gtcggcgctt
      901 acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat tatcaggctg
      961 cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg tataacctgg
     1021 ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct ctcgacctgt
     1081 atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt gagactgaag
     1141 atttactgta gagataaaaa caagggccgg ggaatttcat tcggcccata ttctctttat
     1201 tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg gtcatgacca
     1261 catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa gtgacatttc
     1321 tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc atgatgattg
     1381 aggtcctgga aaacttcgcg ccattcaagc tgcgttttat cggtgatatc tgccagctcc
     1441 tgccataagc tttctttaat cacccgcata aagacgctgt cagattcttc tttcaggctc
     1501 tcttcatagc tcatggcgaa ctcttccagg tcacgtctta cagctcgcgt cagctcatcg
     1561 acctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg ttccaccagt
     1621 gcgtcgatat cacgttcgcc attgcgcagg cgttcgctca gtgacgcgac cagttcacgg
     1681 taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga ctataataat
     1741 agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat taatgtatga
     1801 ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg cggacgtttg
     1861 ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc gtattgaaaa
     1921 caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa tccaaagtac
     1981 agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc aaagagaagt
     2041 attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc cacgtacgta
     2101 actacaccat cggtgacgtg atcgcccgct accagcgtat gctgggcaaa aacgtcctgc
     2161 agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg aaaaacaaca
     2221 ccgctccggc accgtggacg tacgacaaca tcgcgtatat gaaaaaccag ctcaaaatgc
     2281 tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa tactaccgtt
     2341 gggaacagaa attcttcacc gagttgtata aaaaaggcct ggtatataag aagacttctg
     2401 cggtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt atcgacggct
     2461 gctgctggcg ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg tttatcaaaa
     2521 tcactgctta cgctgacgag ctgctcaacg atctggataa actggatcac tggccagaca
     2581 ccgttaaaac catgcagcgt aactggatcg gtcgttccga aggcgtggag atcaccttca
     2641 acgttaacga ctatgacaac acgctgaccg tttacactac ccgcccggac acctttatgg
     2701 gttgtaccta cctggcggta gctgcgggtc atccgctggc gcagaaagcg gcggaaaata
     2761 atcctgaact ggcggccttt attgacgaat gccgtaacac caaagttgcc gaagctgaaa
     2821 tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa agcggttcac ccattaacgg
     2881 gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggagtacggc acgggcgcag
     2941 ttatggcggt accggggcac gaccagcgcg actacgagtt tgcctctaaa tacggcctga
     3001 acatcaaacc ggttatcctg gcagctgacg gctctgagcc agatctttct cagcaagccc
     3061 tgactgaaaa aggcgtgctg ttcaactctg gcgagttcaa cggtcttgac catgaagcgg
     3121 ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt aaagtgaact
     3181 accgcctgcg cgactggggt gtttcccgtc agcgttactg gggcgcgccg attccgatgg
     3241 tgacgctgga agacggtacc gtaatgccga ccccggacga ccagctgccg gtgatcctgc
     3301 cggaagatgt ggtaatggac ggcattacca gcccgattaa agcagatccg gagtgggcga
     3361 aaactaccgt taacggtatg ccagcactgc gtgaaaccga cactttcgac acctttatgg
     3421 agtcctcctg gtactatgcg cgctacactt gcccgcagta caaagaaggt atgctggatt
     3481 ccgaagcggc taactactgg ctgccggtgg atatctacat tggtggtatt gaacacgcca
     3541 ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca ggcatggtga
     3601 actctgacga accagcgaaa cagttgctgt gtcagggtat ggtgctggca gatgccttct
     3661 actatgttgg cgaaaacggc gaacgtaact gggtttcccc ggttgatgct atcgttgaac
     3721 gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa ctggtttata
     3781 ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag gtgatggttg
     3841 aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg gctgatatga
     3901 ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa cgtgtctgga
     3961 aactggttta cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt gatgcgctga
     4021 ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac gatcgctaaa gtgaccgatg
     4081 atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag ctgatgaaca
     4141 aactggcgaa agcaccaacc gatggcgagc aggatcgcgc tctgatgcag gaagcactgc
     4201 tggccgttgt ccgtatgctt aacccgttca ccccgcacat ctgcttcacg ctgtggcagg
     4261 aactgaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac gaaaaagcga
     4321 tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt gccaaaatca
     4381 ccgttccggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag gaacatctgg
     4441 tagcaaaata tcttgatggc gttactgtac gtaaagtgat ttacgtacca ggtaaactcc
     4501 tcaatctggt cgttggctaa gcgagggagg aagcgtgcga tatctggcaa cattgttgtt
     4561 atctctggcg gtgttaatca ccgccgggtg tggctggcat ctgcgtgata ccacgcaggt
     4621 tccttccact atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc cattaagccg
     4681 tgcggtgcgt aaccagttac gtctgaatgg tgtcgagttg cttgataaag aaaccacgcg
     4741 taaggacgtt ccatccttgc gtttgggtaa agtgagcatc gcgaaagata ccgcatcggt
     4801 attccgtaac ggtcaaacag cagagtatca tatgatcatg acggttaatg cgaccgtgtt
     4861 gatccccggc cgtgatatct acccgattag cgccaaagtc ttccgttcgt tcttcgataa
     4921 cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag agatgtacga
     4981 ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg atattcgttc
     5041 cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac gcgtctccac
     5101 cacgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca gctcaatgaa
     5161 gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca ggaaagccag
     5221 gacgctgttc gtcaggtagc tgcggcacaa ggattcgaag aacaccacac tttttccatt
     5281 gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggccatgag tctgtttgcc
     5341 agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc gatcaatgaa
     5401 caacttctca cactcaccgg acttctgcat gacgacctgc tgttgatcgt ccgcggtaat
     5461 aaattaagca aagcgcaaga aaattccgcc tggtttactg ctcttgcgaa tcgcagcgtg
     5521 caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc tgcgcgcgca
     5581 aaacagctca atttagaact ggatgacgcg gcgaatcagg tgctctgcta ctgttatgaa
     5641 ggaaacctgc tggcgctggc ccaggcactg gagcgtttat cgctactctg gccggacggc
     5701 aaactgacat taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt cacccctttt
     5761 cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cgttgcatat tcttcagcaa
     5821 ctgcgtctgg aaggcagcga gccggttatt ttgttgcgca cattacaacg tgaactgttg
     5881 ttactggtta acctgaaacg ccagtctgcc catacgccac tgcgtgcgtt gtttgataag
     5941 catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg cttaagccag
     6001 ccgcagttac gtcaggctgt gcaactcctg acacgaacgg aactcaccct caaacaagat
     6061 tacggtcagt cagtgtgggc agagctggaa gggttatctc ttctgttgtg ccataaaccc
     6121 ctggcggacg tatttatcga cggttgatat gaaatcttta caggctctgt ttggcggcac
     6181 ctttgatccg gtgcactatg gtcatcttaa acccgtggaa acgctggcga atttgattgg
     6241 tctgacgcgg gtcacaataa tccctaataa tgttcctccg catcgtcccc agccggaagc
     6301 gaacagcgtg cagcgtaaac acatgcttga actggcgatt gccgacaagc cattatttac
     6361 tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa cactgaaaga
     6421 gtggcggcag gaacaagggc cggacgtgcc gctggcgttt attattggtc aggattcatt
     6481 gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac atttgatcgt
     6541 ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg caataccagc aatggctgga
     6601 agatcatttg acacataacc cggaagatct tcaccttcag cctgccggta aaatttatct
     6661 ggctgaaaca ccgtggttta acatctcggc gaccatcatc cgcgaacgtt tgcaaaacgg
     6721 tgaatcgtgt gaggatttat tgccggaacc ggtattgact tacattaacc aacaaggctt
     6781 gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat tcgtcatggc
     6841 gaaacacaag cgaatgtcga tggtctgtac agcggtcatg cgcccacacc cctgaccgca
     6901 cgcggtattg agcaagcgca aaatctgcat acactgctac atgatgtttc ctttgacctg
     6961 gttttatgca gtgaactgga acgggcacag cataccgcgc gactggttct cagtgaccgc
     7021 cagctccccg ttcatatcat ccctgaactc aacgaaatgt tttttggtga ctgggagatg
     7081 cgacatcatc gcgacctcat gcaagaagat gccgaaaact atagcgcgtg gtgcaatgac
     7141 tggcagcatg ctatcccacg aacggtgaag gatttcaggc attttcacaa cgtgtggaac
     7201 gctttatcgc aaggcttagt gaatatcagc actatcagaa tattttaatc gtcagccatc
     7261 agggcgtact gagtctgtta attgctcgtt taattggcat gcctgccgag tctatgtggc
     7321 attttcgcgt tgaccagggg tgttggagcg ccattgatat caaccaaaaa ttcgcgacgc
     7381 tacgcgtcct caatagccgt gccatcgggg tcgagaatgc atgacttttc tgttttttta
     7441 cgggtaagcc gcaacgacca ttgacagccc cgggcaggct gatattctcc gcagccagac
     7501 tttttccgcc agacacgact ttgtagaaat tgttttacaa aaatggcgat gcaatctgcg
     7561 gcgcggggtg ggatgatagc ccactttcga aagccgattc ggcgacaatt gtcccgaaat
     7621 cgcctctggt tcaggtatac tgacagacca tttttatcta tttgattcac ccagggggaa
     7681 aacttgcagg gtaaagcact ccaggatttt gttatcgaca aaattgatga cctcaaaggt
     7741 caggacatca tcgccttaga cgttcaaggc aaatccagca tcaccgactg catgatcatc
     7801 tgtacgggta cgtccagccg tcatgttatg tccattgctg accacgttgt gcaggagtct
     7861 cgcgcagcgg gcctgttacc gctcggcgta gaaggtgaaa acagcgccga ctggattgtc
     7921 gtggatttgg gcgatgtgat tgtccatgtc atgcaggaag agagccgtcg cctgtatgaa
     7981 ctggaaaaac tctggagtta atgcgtgaag ctgcaacttg tcgccgtggg aacgaaaatg
     8041 ccggactggg tacaaaccgg ttttaccgag tacctgcgtc gttttccgaa agatatgccc
     8101 ttcgagctga ttgaaattcc ggccggaaaa cgcggcaaga atgcggacat caagcgcata
     8161 ctcgacaaag agggtgagca gatgttggcg gccgcaggca aaaaccgcat tgtcaccctc
     8221 gatattccag gcaagccctg ggatacgccg cagttagccg ctgagctgga acgctggaag
     8281 ctggatggtc gcgacgtcag tctactgatt ggcgggcctg aagggttgtc gcctgcctgt
     8341 aaagcggcgg cggagcagag ctggtcgctg tcggcgctta ccctccccca tccgctggtt
     8401 cgcgtgctgg tcgcagagag tctgtaccgg gcgtggagca tcaccaccaa ccatccttat
     8461 caccgtgagt gataagggag ctttgagtag aaaacgcagc ggatgaaact acagaactct
     8521 tttcgcgact atacggcaga gtccgcgctg tttgtgcgcc gggcgctggt cgcctttttg
     8581 gggattttgc tgctgaccgg cgtgcttatc gccaacctgt ataatctgca aattgttcgc
     8641 tttaccgact accagactcg ctctaatgaa aaccgcatta agctggtgcc tatcgcgccc
     8701 agccgcggca ttatctacga ccgtaatggt atccctctgg ccctcaaccg cactatctac
     8761 cagatagaaa tgatgccaga gaaagtcgat aacgtgcagc aaacgctgga cgctttgcgc
     8821 agcgtggttg atctgaccga tgacgatatt gctgcattcc gaaaagagcg cgcacgttca
     8881 caccgtttca cctctattcc ggtgaaaact aacctgaccg aagtacaagt agctcgcttt
     8941 gccgtcaatc agtaccgttt tccgggtgtc gaagttaaag gctataaacg tcgttactat
     9001 ccttacggtt cggcgttgac ccacgtcatc ggctatgtgt cgaaaatcaa cgataaagac
     9061 gtcgaacgcc tgaataatga cggcaaactg gccaactatg cggcaacgca tgatatcggt
     9121 aagctgggca ttgagcgtta ctatgaagac gtgctgcacg gtcagaccgg ttatgaagag
     9181 gttgaagtta acaaccgtgg gcgtgttatc cgccagttaa aagaagtacc accgcaagcc
     9241 ggacacgata tttacctgac gctggatctc aaactccagc aatatattga aacgctgctg
     9301 gcgggtagcc gcgcagctgt ggttgtcacc gatccgcgta caggtggggt actggcgctg
     9361 gtttccacgc ctagttatga cccaaacctg tttgttgacg gtatctccag caaagattat
     9421 tccgccttgt tgaacgaccc gaatacaccg ctggtgaacc gcgccacaca gggggtttat
     9481 cctcccgcgt ctacagttaa accctatgtg gcggtttcgg cattgagcgc cggggtgatc
     9541 acgcgcaata cgacgctgtt tgacccaggc tggtggcaac tgccaggttc ggaaaaacgt
     9601 tatcgtgact ggaaaaaatg gggccacggg cgtctgaatg tcacaagatc gctggaagaa
     9661 tctgcggata ccttcttcta tcaggtggcc tacgatatgg ggatcgatcg cctctccgaa
     9721 tggatgggta aattcggtta tggtcattac accggtatcg acctggcgga agaacgttcc
     9781 ggcaacatgc ctacccgcga atggaaacag aaacgcttta aaaaaccgtg gtatcagggt
     9841 gacaccattc cggttggtat cggtcagggt tactggacag cgaccccaat ccagatgagt
     9901 aaggcactga tgatcctgat taatgacggt atcgtgaagg ttcctcattt gctgatgagc
     9961 accgccgaag acggcaaaca ggtgccatgg gtacagccgc atgaaccgcc cgtcggcgat
    10021 attcattccg gttactggga gctggcgaaa gacggtatgt acggtgttgc taaccgccct
    10081 aacggtacgg cgcataaata ctttgccagc gcaccgtaca aaattgcggc gaaatccggt
    10141 accgctcagg tcttcggtct gaaagcgaac gaaacctata atgcgcacaa aattgccgag
    10201 cgtttacgtg accacaaact gatgaccgcc tttgcgccat acaacaatcc gcaagtggct
    10261 gtcgccatga ttctggagaa cggtggtgcg ggtccggcgg ttggtacact gatgcgccag
    10321 atcctcgacc acattatgct gggtgataac aacaccgatc tgcctgcgga aaatccagcg
    10381 gttgccgcag cggaggacca ttaatcatga cggataatcc gaataaaaaa acattctggg
    10441 ataaagtcca tctcgatccc acaatgctgc tgatcttact ggcattgctg gtttacagcg
    10501 ccctggttat ctggagcgcc agcggtcagg atattggcat gatggagcgt aaaatcggcc
    10561 aaatcgcgat gggtctggtc atcatggtgg tgatggcgca aattcctcca cgcgtttatg
    10621 aaggctgggc cccctatctc tatatcatct gtattatttt gctggtggcg gtagatgctt
    10681 tcggtgccat ctctaaaggt gctcaacgct ggctggacct cggtattgtt cgttttcagc
    10741 cgtcggaaat tgccaaaata gccgtaccac tgatggttgc gcgctttatc aaccgcgacg
    10801 tttgcccgcc atcgttgaag aacactggca tcgcgctggt gctgatattt atgcccacgc
    10861 tgctggtggc tgcacagcct gacctgggaa catcaatcct cgttgcgctt tccggtctgt
    10921 ttgtactgtt cctctctggc cttagctggc gtctgattgg cgtcgcagta gtgctggtgg
    10981 cggcgttcat tccgattctg tggttcttcc tgatgcatga ttaccagcgc cagcgcgtaa
    11041 tgatgctcct ggacccggaa tcagacccac tcggcgcggg ctatcacatt attcagtcta
    11101 aaattgctat tggctccggc ggattacgcg gcaaaggctg gctgcacggc actcagtcac
    11161 agcttgaatt tctccccgaa cgccataccg actttatctt cgcggtactg gcggaagagc
    11221 tgggattagt gggcattctg attctgctcg ctctctacat tctgctgatc atgcgcgggc
    11281 tgtggatagc cgccagagcg caaaccacct ttggtcgcgt catggctggt ggcttaatgc
    11341 tgatattatt cgtttatgtc ttcgtaaata ttggtatggt aagcggtatt ctgccggttg
    11401 taggggttcc gctcccactg gtcagttatg gaggatcggc gctaattgtg ctgatggctg
    11461 ggttcgggat tgtaatgtca atccacaccc acaggaaaat gttgtcgaaa agcgtgtaag
    11521 aggtgcgcaa tgcgtaagca gtggctcggg atctgcatcg cggcaggaat gctcgcggca
    11581 tgtacaagcg atgatggtca gcaacagacg gtaagtgtac cgcagcctgc ggtatgtaac
    11641 ggccccatag ttgaaattag cggggcggac ccgcgtttcg aaccactgaa cgcgacggca
    11701 aatcaggatt accagcgcga cggtaaaagc tacaaaatcg tgcaggatcc gtctcgattt
    11761 agccaggcgg gactggcggc aatctatgat gccgaaccag gcagtaacct gacggcctct
    11821 ggcgaagctt tcgatccgac acagctgacg gcggcccatc caacgcttcc gatccccagc
    11881 tacgccagaa tcactaacct ggctaacggg cgaatgatcg tggtgcgcat taatgatcgc
    11941 ggcccttacg gcaacgaccg cgttatttcg ctttctcgcg cggcagctga ccgtcttaac
    12001 acgtcaaaca acaccaaagt tcgtatcgat ccgattattg tcgcccagga tggttcgctt
    12061 tctggtcctg gtatggcttg taccacagtc gccaaacaga cttacgccct gcctgcacct
    12121 cccgatttaa gcggtggcgc gggaacaagt tcagtgtctg gcccgcaggg tgacattctt
    12181 ccggtcagta attcgacgct aaaaagcgaa gatccgaccg gcgcgccggt aaccagtagc
    12241 ggtttcctcg gcgcaccaac gaccttagcg cctggtgtac tggaaggcag cgaaccgacg
    12301 cctgcgccac agcccgttgt tacagctccg tcgacaacgc ctgcaacctc gcctgcaatg
    12361 gtgacaccgc aagccgcctc gcaaagcgcc agcggcaact ttatggtgca agtcggggcc
    12421 gttagcgatc aggctcgtgc gcaacagtac caacagcaac tgggacagaa gttcggcgtc
    12481 cccggtcgcg taactcaaaa tggcgcggtc tggcggatcc agcttggccc cttcgccagc
    12541 aaagccgaag ccagtacctt gcagcaacgt ttgcaaaccg aagcccaatt acagtcattt
    12601 attaccaccg cgcagtagcg taaagcaggc atctgatgtg tcaatttctg taaatgacgt
    12661 taacaaagtc acgcggaaag tcagatgcct gccggtagtg catttgctat agtagggcac
    12721 tttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt tttccgctcg
    12781 tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct ctgctgcaca
    12841 tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg atgcggagtc
    12901 ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg cagatgtccg
    12961 ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc aagcaatgaa
    13021 agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat gggccaccgg
    13081 taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc aggttccggt
    13141 ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg tcgccatggc
    13201 tgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct acgttaacgc
    13261 cctgggcctg aaaaacaccc acttccagac ggtacatggt ctggatgctg atggtcagta
    13321 cagctccgcg cgagatatgg cgctgatcgg ccaggcattg atccgtgacg taccgaatga
    13381 atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc tgaaccgtaa
    13441 cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac acactgacaa
    13501 agcaggttac aaccttgttg cttctgcgac tgaaggccag atgcgcttga tttctgcggt
    13561 gatgggcgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc taacctgggg
    13621 cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg cctctgaacc
    13681 ggtttggttt ggtgattctg atcgcgcttc gttaggggtt gataaagacg tgtacctgac
    13741 cattccgcgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca gcagtgaatt
    13801 gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc ttgatggcaa
    13861 aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta acttcttcgg
    13921 caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa aattgaacac
    13981 ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaaactc ccgccttctg
    14041 gcgggagttg ctatttaatt acgttacgcc ggagctgaca tgaaaaccaa acttaacgaa
    14101 ctgcttgaat tccctactcc ttttacttac aaagttatgg ggcaggcgtt acctgagctg
    14161 gttgatcagg tggttgaagt ggtacagcgc catgcgccag gtgactacac cccaacggta
    14221 aaaccaagca gcaaaggcaa ctaccactcg gtatctatca ctatcaacgc cactcatatc
    14281 gagcaggttg aaacactgta tgaagaactg ggcaaaatcg atattgtccg catggttctg
    14341 taactcgctt ctccgttacc cggctcctgg tcgggtaact cccctctcct cgctgtgata
    14401 tacttttccc ccacttttac tcattctcca cggagatgcc gttttgtatc aggataaaat
    14461 tcttgtccgc cagctcggtc ttcagcctta cgagccaatc tcccaggcta tgcatgaatt
    14521 caccgatacc cgcgatgata gtacccttga tgaaatctgg ctggtcgagc actatccggt
    14581 attcacccaa ggtcaggcag gaaaagcgga gcacatttta atgccgggtg atattccggt
    14641 gatccagagc gatcgcggtg ggcaggtgac ttatcacggg ccggggcaac aggtgatgta
    14701 tgtgttgctt aacctgaaac gccgtaaact cggtgtgcgt gaactggtga ccttgcttga
    14761 gcaaacagtg gtgaataccc tggctgaact gggtatagaa gcgcatcctc gggctgacgc
    14821 gccaggtgtc tatgttgggg aaaagaaaat ttgctcactg ggtttacgta ttcgacgcgg
    14881 ttgttcattc cacggtctgg cattaaacgt caatatggat ctttcaccat ttttacgtat
    14941 taatccttgt gggtatgccg gaatggaaat ggctaaaata tcacaatgga aacccgaagc
    15001 gacgactaat aatattgctc cacgtttact ggaaaatatt ttagcgctac taaaaaatcc
    15061 tgacttcgaa tatattaccg cttaattcca tacctcaatg gcccaattta cactgggtca
    15121 ttactcataa tacctgctaa tgctttacat ctccttcgcg tttgtatatt ttctttatca
    15181 cttgcagaaa tgcggtgcca cgccttgctc cgtaactcac cccattgatt aacctattga
    15241 aatagttgat tatggtgagc aaaagcattc cgttccaatt agacataaga ttattgtaat
    15301 tttttaaata ttatttttcc atgaataaaa ttggagtaag tgcgtggata gtaataatca
    15361 aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat ttacaacctt
    15421 acgaaatatt gatcttaacc ttctgactat ttttgaagct gtatatgtac ataaagggat
    15481 cgttaatgca gcgaaagtgc ttaatctgac cccctcggca atcagtcagt ctattcagaa
    15541 actgcgcgtt atattccctg acccattgtt tattcgcaaa ggccagggtg tcactcctac
    15601 cgcatttgcg atgcatctac atgagtatat cagtcagggc cttgagtcca ttcttggcgc
    15661 gctggatatc gaaggaagct atgataagca acgaacgata actattgcga ccactccctc
    15721 ggtcggagcc ctgttccttc ctgtcatcta tcgggcgatt aaaactcact atccgcagct
    15781 tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa ctcagtcagt ttcaaaccga
    15841 tctcatcatc gataacatgt tttgcaccaa tcgtacggtg caacatcatg ttctgttcac
    15901 cgacaatatg gtgttaattt gccgcgaggg aaatccacta ctctctttag aagatgacag
    15961 agagactatc gacaacgctg cgcatgtact tctgttaccg gaagagcaaa atttcagcgg
    16021 tctgcggcag agagttcaag aaatgtttcc ggaccggcaa atcaatttca ccagctacaa
    16081 cattttgaca atcgctgcac tggttgccaa cagtgacatg ttagcgatta ttcccagccg
    16141 tttttataac ctgtttagcc gctgctggcc gctggaaaaa ctgccttttc cgtccttaaa
    16201 tgaggagcaa atagatttct ctatccacta caacaaattc agcctgcgtg atcccatcct
    16261 gcacggagta atagatgtta tccgtaatgc attttgaaaa agtattgtta cagccagatc
    16321 acagcgcaga acgataaaat ggcacaatcc aacaacaatt ttacattttc gcgaccgctt
    16381 tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa aaatagttga
    16441 taattacaac aatctattga attgaaacgc tttccttcgt aattcgcaac tggaacacgc
    16501 acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat gccgataaga
    16561 tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg cgcaagccgg
    16621 aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc aaagccgcaa
    16681 tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac ctggcggaat
    16741 gcttcaacca cggcacagca acgtttatga tcctcggcgc tatttgtacc cgccgttgtc
    16801 cgttctgtga tgttgcccac ggtcgcccgg tagctcctga tgccaatgaa ccagtgaaac
    16861 tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc gttgaccgtg
    16921 atgacctgcg cgatggcggt gcccagcact ttgcggattg cattactgcc attcgggaaa
    16981 aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgtggtcgt atggatcgtg
    17041 ctctggatat tctgactgca acgccaccag atgtgttcaa ccataacctg gaaaacgtac
    17101 cgcgtattta ccgtcaggta cggcctggtg cagattacaa ctggtcgctg aagctgctgg
    17161 aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg gtgggactgg
    17221 gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat ggtgtgacga
    17281 tgttaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt caacgttacg
    17341 ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc tttacccatg
    17401 ctgcatgcgg tccgtttgtc cgctcttctt accacgccga tttgcaggcg aaagggatgg
    17461 aagttaagta atcctataat aattacttac atctttctga taaaaaaccc gctttttgaa
    17521 gcgggttttt tgtatcgaac aagatattga gggagcgtcc tgctcgccac gtcactcttt
    17581 atgagagagc ttttcagcct gaagatcaac gtctgcgcct tttttcgccg cagcatcgtc
    17641 atcattcatc gccttcttga accctttaat ggccgctcca aggtctccgc ccagcgtacg
    17701 taacttctta gtcccaaaca gcagaacgac cagcgccgca actaccagca gtttggtaat
    17761 actaatctca cccatagata ccttcttgac atataaccgg caacagaacg cgccttaaaa
    17821 accagagaat attaacggtc attcgacgcg cgcacacaat agcaatctgt aacaaggtga
    17881 atcaagcgcc gagtaaaaaa acatcataat aattgcggcg gcgcaaagcg acggttgttt
    17941 aagacgggca gttgcgcgcg cacctgacgc acacgttcgg gcgtcacttc cgccataatg
    18001 agtgcaggca tttctgacgc tgccgcaatg gtgacgccaa agggatctat aatccggctt
    18061 tgaccgatat ttttgttccc gcactccccc gccgccacca tataacaggt ggtatccagc
    18121 gcacgagcgg caagcaacgt tgaccagtga tgctctttga gcggcccgcg aacccaggcg
    18181 gcaggaagta ccaggatttc agctccctgt aatgcctgtg ccagcgccag ctctggaaag
    18241 cgtaagtcat aacaggtcat cagaccgacc ttcatccctt ccacctccag taacggagcg
    18301 atttcattac cagcatcaac acggcgtgat tcctgaatgg caaatgcatc atagagatgc
    18361 aatttggcat aacgggcgac gatgtttcct gcctgtagcg ccaccagcat attccatgcg
    18421 cgccccggcg ttgaagggac atgaatcgtc agaattgtcg tcatcatgtt acgtttactt
    18481 tctcgccgta aacgtccgag gaattcgcct tccagcagct gtgctgattt aaccgataga
    18541 tctgcatcat gatcatcgcg cgccagcaat gcttccggca ggacaaacag cgatacgtcg
    18601 ttttccgccg cctgcgccat caacgaggca caaatctcag cgttcttttc ccacacagat
    18661 gtaacagcaa actgtcctgc ggcaaccagc attgccacct cctgtcagag tcgtaaaatt
    18721 cgccaacgac gattattcgg cgttacactt atcactcata caaatcaaat agcaggattt
    18781 tgcagtgtta caacttcttt tagcagtttt tattggcggt ggtacgggaa gcgtggcgag
    18841 atggctgtta agtatgcgat ttaacccgct gcatcaggcg attccgttgg ggacgctggc
    18901 agcaaacctg attggggcat tcatcatagg aatgggattc gcctggttca gcaggatgac
    18961 gaacattgat ccagtgtgga aagtattaat caccaccgga ttttgtggcg gtctaacaac
    19021 cttctcaaca ttttcggcag aagtggtgtt tttgttacaa gagggccgct ttggctgggc
    19081 gttactgaac gttttcgtca accttctggg gtcttttgcc atgaccgcac tggctttctg
    19141 gctgttttcg gcctcaaccg cacactaaag accaaaaaaa acccgctgat taagcgggtt
    19201 ttgaattctt gctgacgtat cttacagagc gattacgttt gcagcagaag ggcctttggc
    19261 accgttagtg atttcgaact ctacgcgctg accttcagca agagttttaa aaccattagt
    19321 ctggattgca gagaagtgta cgaacacgtc tttgctgccg tcttccggag taatgaaacc
    19381 gaatcctttg gactcattaa accacttaac gttaccttta atcttagaca tcaaaattac
    19441 ctttacatga aaaatagaca caaatgctgt gtcggttacc agtacaccaa ttgtggtacg
    19501 ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg cgttattttt gatgttactt
    19561 attttatcta ttgattttaa agaagttact aaaacttcat ttgtctcaaa actgaaagcg
    19621 catccaggca aagtacacat tgccattgtt gtaggtaccc ggaatgtagg tcatctgaaa
    19681 agtcactggg ccataaccca cggaggccaa tggcagtaga accgggagag ggatgtaatt
    19741 ccagttatcg cgtgccgtta cgccagcggt gaatcccaga cctaaatgaa aattttcatc
    19801 cgccagcggt cgccaggtac tttcccatcc gtatccggca atcggttccc atttgttcca
    19861 cgagtcctta aatgccatgg catacaggcc atgccagttt cctttttcat cccaacgcga
    19921 ctgaccaaaa ccgccacccc acggtcgctc gttatagcga tcggtttttt ctttgtcgta
    19981 agcgaaacgt gcatgccagg tgatggcagg aatatataaa tcataatgtt caggctgttg
    20041 ccaggtttgt gcaatatttt ctctaaacgt tgtcatccac tcatctgcgt tagcaaaaac
    20101 tttaccaacg ctgattaact gaataaaaaa aaaggaaaag atagcgacat atttactcac
    20161 gttcatttgt gaccataaaa catttatcaa aaatctacta ctagcatagc aaagctacaa
    20221 ttaacacaac cttaatagac ccaacataaa gaataatctg aatagctgct ctatttattt
    20281 ccgcttgttc aagtt
//
LOCUS       DAOFQD010000081        20266 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.081, whole genome shotgun sequence.
ACCESSION   DAOFQD010000081 DAOFQD010000000
VERSION     DAOFQD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.081"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..715)
                     /locus_tag="QBF46_003080"
     CDS             complement(<1..715)
                     /locus_tag="QBF46_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HDQ6675230.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQ"
     gene            complement(712..930)
                     /gene="ybdZ"
                     /locus_tag="QBF46_003081"
     CDS             complement(712..930)
                     /gene="ybdZ"
                     /locus_tag="QBF46_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDQ6675231.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCVLPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            complement(933..2135)
                     /gene="fes"
                     /locus_tag="QBF46_003082"
     CDS             complement(933..2135)
                     /gene="fes"
                     /locus_tag="QBF46_003082"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDQ6675232.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWKTQLNANWRGSYCFIPTERDDIF
                     SVPSPDRLELREGWRKLLPQAIADPLNLQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKKSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            2378..4618
                     /gene="fepA"
                     /locus_tag="QBF46_003083"
     CDS             2378..4618
                     /gene="fepA"
                     /locus_tag="QBF46_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDQ6675233.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            4793..5413
                     /gene="entD"
                     /locus_tag="QBF46_003084"
     CDS             4793..5413
                     /gene="entD"
                     /locus_tag="QBF46_003084"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDQ6675234.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(5535..5687)
                     /gene="hokE"
                     /locus_tag="QBF46_003085"
     CDS             complement(5535..5687)
                     /gene="hokE"
                     /locus_tag="QBF46_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDQ6675235.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            6140..7258
                     /locus_tag="QBF46_003086"
     CDS             6140..7258
                     /locus_tag="QBF46_003086"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDQ6675236.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            7324..7572
                     /locus_tag="QBF46_003087"
     CDS             7324..7572
                     /locus_tag="QBF46_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDQ6675237.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVSTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            7637..8005
                     /locus_tag="QBF46_003088"
     CDS             7637..8005
                     /locus_tag="QBF46_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDQ6675238.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPSEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            8099..8752
                     /gene="nfsB"
                     /locus_tag="QBF46_003089"
     CDS             8099..8752
                     /gene="nfsB"
                     /locus_tag="QBF46_003089"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDQ6675239.1"
                     /translation="MDIIYVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            8860..10107
                     /gene="ybdG"
                     /locus_tag="QBF46_003090"
     CDS             8860..10107
                     /gene="ybdG"
                     /locus_tag="QBF46_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDQ6675240.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGVKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(10175..11551)
                     /gene="pheP"
                     /locus_tag="QBF46_003091"
     CDS             complement(10175..11551)
                     /gene="pheP"
                     /locus_tag="QBF46_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDQ6675241.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(11653..14796)
                     /gene="cusA"
                     /locus_tag="QBF46_003092"
     CDS             complement(11653..14796)
                     /gene="cusA"
                     /locus_tag="QBF46_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDQ6675242.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLGEVKNALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(14808..16031)
                     /gene="cusB"
                     /locus_tag="QBF46_003093"
     CDS             complement(14808..16031)
                     /gene="cusB"
                     /locus_tag="QBF46_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDQ6675243.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(16047..16379)
                     /gene="cusF"
                     /locus_tag="QBF46_003094"
     CDS             complement(16047..16379)
                     /gene="cusF"
                     /locus_tag="QBF46_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDQ6675244.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(16403..17785)
                     /gene="cusC"
                     /locus_tag="QBF46_003095"
     CDS             complement(16403..17785)
                     /gene="cusC"
                     /locus_tag="QBF46_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDQ6675245.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            17942..18625
                     /gene="cusR"
                     /locus_tag="QBF46_003096"
     CDS             17942..18625
                     /gene="cusR"
                     /locus_tag="QBF46_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDQ6675246.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            18615..20063
                     /gene="cusS"
                     /locus_tag="QBF46_003097"
     CDS             18615..20063
                     /gene="cusS"
                     /locus_tag="QBF46_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDQ6675247.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         4914 a         5431 c         5119 g         4802 t
ORIGIN      
        1 gctggcggaa ttccccgtcg gtaaattcca gtttcaggcg cagaatatct gccgaggcgc
       61 tgcgccccgg taaaggtgcc ggagaaagtg acgcgggcgg tggcagttga cgacgctgtt
      121 ccgcccagaa tgccgcatca cgctgccagg cttcgctttc gcggtattgc tggtactctt
      181 ccactacatc agcgaaaggc gtaaacggcg aagcaggcgt tggttcgcca cgcagccatg
      241 tgcagtaaat attggcgatc tggcgggtaa tggccgggaa actaaagcca tcgaccagca
      301 aatggtgata acgctgatac cagtaccagc ggttatctgc cacctgaatg agctgatgaa
      361 agaccagtgg tttaccgcta tcgacgcgca gatcttgctg caaatcggcc tgcattaatg
      421 cctgcgcagt accgtgcgga tcaatgttgg ttcgcaggtc gataatttct ggcagttcga
      481 acgtcagcgc atcatcgacc cactgccaga cttcgccgtt atcttccgta aaacgcatcc
      541 gcagcgtatc tgcttgcgct agtccggcaa ccaccgcgcg ggccagtaat ggcgaatcaa
      601 cctctccggt taactcaacg taatgcgcca cgctccaggc ggagggtaat tctgacagtt
      661 tttctgccat ccagatgccg ggctgtgcgg cgaccaaagg taaatgctgg ctcattgtgc
      721 ctcctgcaac tgggtaaaat tcgtcggtgt cagagtacgc cagtgggctt ccagccactg
      781 ctggcaggac gcctgtgact gcggctgaca cacaatgtcc cagcctgccg gtaagacgca
      841 ttgttgcggc cacagactga attgcccctg cgcattgcgc aatatgtaaa acgctccctg
      901 cggatcatcg aagggattac tgaatgccat attcaactcc tgtcatggaa aagtggttgc
      961 cagaggtcga ttagcccctg catcaagcca ccgcgccaac aaagcgcatc atgtccgccg
     1021 tcaacctgac gccagaaaat ggattctttt atggggtgta attgcgcata cagcgcctga
     1081 ttggcccgca tgatcatcgg ctcgcgaata cccgcttcca gcacaatgcg cagaccttcg
     1141 gcgctaactt caccagcttt tagcttttca agtaacacgc cctcttgctg cccgccccga
     1201 tgcggccacc agtacgatcc tgactggctt aatacacagc caaagcgttc aggccagtgc
     1261 agtccggcat acagcgcgga aagcccacca aaactctgcc cggcaaccac ggtgcgatcg
     1321 gcacgatcgc taaaaggggc aatagctttc accaggggta ataactcttg ctgtactgcg
     1381 agccagaaat ccgcattaca cggcagttcg tgggcgcggt gcgtggtgtc gatagcgtcg
     1441 atcaacacat acacggcggg aggaagttgc tgacgatggg tcagcgaagt cagcactggc
     1501 cagacgggca tactttgcgc ccaaaattcg ccatcgagca aaactgccag cgggcgttct
     1561 tctgctgttg catcgccggt ggtaaaaatc catacacgcc gtgacttttt caaccgttca
     1621 cttttccaga taatttcttt ggcaggtatt tctggcgctt gcggacaatc ccatcccggt
     1681 tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt gccctcgccc gcctttccag
     1741 ctttgtaggt tcagcggatc ggctatcgcc tggggtaata gttttcgcca gccttcgcgc
     1801 aattcgaggc gatcggggga tggtacagaa aaaatgtcat cgcgttcggt gggaataaag
     1861 cagtagctgc cgcgccagtt ggcattgagt tgtgtcttcc actgccagac gttagtgcct
     1921 gcaattcgct gcatcgactg gggctggctg ttctgatggt gatcggtcac accagtgatg
     1981 tagacccata cgcgctttat cgtcgagtat tcttcagaac cttggggatc acgccaccag
     2041 aaagtgacct caaacatttc gtcattcaga cgctgccatt ccgggccatg tttcgactgc
     2101 caccagctct cacttcctac ttttaacgcc gtcacaccat aaccccatgt ttactgtgca
     2161 atttttcatt gattgcagaa atatattgat aatattattg ataactattt gcatttgcaa
     2221 tagcgtaatg gcgcgccgtg ggaagcgcgg acattaatta accaactgca ctgcgtgtct
     2281 ttcaggatca aaggttttcg cggtagcggg atgcgtcgtg ttgatgacga ccatgcccga
     2341 cagttgcaat tcgtggcaaa aatgcaggaa taaaacaatg aacaagaaga ttcattccct
     2401 ggccttgttg gtcaatctgg ggatttatgg ggtagcgcag gcacaagagc cgaccgatac
     2461 tcctgtttca catgacgata ctattgtcgt taccgccgcc gagcagaact tacaggcgcc
     2521 tggcgtttcg accatcaccg cagatgaaat ccgcaaaaac ccggttgccc gcgatgtgtc
     2581 ggagatcatc cgtaccatgc caggcgttaa cctgaccggt aactccacca gtggtcagcg
     2641 tgggaataac cgacagattg atattcgcgg tatgggtccg gaaaacacgc tgattttgat
     2701 tgacggcaag ccggtaagca gccgtaactc ggtgcgtcag ggctggcgtg gcgagcgcga
     2761 tacccgtggt gatacttcct gggtgccacc tgaaatgatt gaacgtattg aagttctgcg
     2821 tggtccggca gctgcgcgtt atggcaacgg cgcggcgggc ggcgtggtta acatcattac
     2881 caaaaaaggc agcggcgagt ggcacggctc ctgggacgca tatttcaatg cgccagaaca
     2941 taaagaggaa ggtgccacca aacgcactaa ctttagcctg accggtccgc tgggcgacga
     3001 attcagcttc cgtttgtatg gcaacctcga caaaacccag gctgacgcgt gggatatcaa
     3061 ccagggccat cagtccgcgc gtgccggaac gtatgccacg acgttaccag ccgggcgcga
     3121 aggggtaatc aacaaagata ttaatggcgt ggtgcgctgg gatttcgcgc cattgcaatc
     3181 gctggaactg gaagcaggtt acagccgcca gggtaacctg tatgcgggcg acacccagaa
     3241 taccaactcc gattcctata cccgctcgaa atatggcgat gaaaccaacc gtctgtatcg
     3301 ccagaactac gcgctgacct ggaacggtgg ctgggataac ggcgtgacca ccagcaactg
     3361 ggtgcagtac gaacacaccc gtaactcgcg tattccggaa ggtctggcgg gcggtaccga
     3421 agggaaattt aacgaaaaag cgacacagga tttcgtcgat atcgatcttg atgacgtgat
     3481 gctgcatagc gaagttaacc tgccgattga tttcctcgtt aaccagacgc tgacgctggg
     3541 tacggagtgg aatcagcaac ggatgaagga cttaagttcc aacacccagg cactgaccgg
     3601 aacgaatacc ggtggcgcta ttgatggcgt gagtgccacc gaccgtagcc cgtattcaaa
     3661 agcagaaatt ttctcgctgt ttgccgaaaa caacatggag ctgactgaca gcaccatcgt
     3721 aacgccgggg ctgcgtttcg atcatcacag tattgtcggc aataactgga gcccagcgct
     3781 gaacatatcg caaggtttag gcgatgactt cacgctcaaa atgggcatcg cccgcgccta
     3841 taaagcgccg agcctgtacc agactaaccc aaactacatt ctctacagta aaggtcaggg
     3901 ctgctatgcc agcgcgggcg gctgctatct gcaaggtaat gatgacctga aagcggaaac
     3961 cagtatcaac aaggagattg gtctggagtt caaacgcgac ggctggctgg cgggcgtcac
     4021 ctggttccgt aacgattatc gcaataagat tgaagcgggc tatgtggctg tagggcaaaa
     4081 cgcagtcggc accgatctct atcagtggga taacgtgccg aaagcggtgg ttgaaggtct
     4141 ggaaggatcg ttaaacgtac cggttagcga aacggtgatg tggactaata acatcactta
     4201 tatgctgaag agtgaaaaca aaaccacggg cgaccgtttg tcgatcatcc cggagtatac
     4261 gttgaactca acgctgagct ggcaggcacg ggaagatttg tcgatgcaaa cgaccttcac
     4321 ctggtacggc aagcagcagc cgaagaaata caactataaa ggtcagccag cggttggacc
     4381 ggaaaccaaa gaaatcagtc cgtacagcat tgttggcctg agcgcgacgt gggatgtgac
     4441 gaagaatgtc agtctgaccg gcggcgtgga caatctgttc gacaaacgtt tgtggcgtgc
     4501 gggtaatgcc cagaccacgg gcgatttggc aggggccaac tatatcgccg gtgccggggc
     4561 gtatacctat aacgagccgg gacgtacgtg gtatatgagc gtaaacaccc acttctgatg
     4621 ctaacgtcag attgttgaca aagtgcgcgt cgttcatgcc ggatgcggcg tgaacgcctt
     4681 atccggccta caaaaatctt gccaattcaa tatattgcag gaccatgtag gcctgataag
     4741 cgtagcgcat caggcagttt tgcgtttgtc atcagtctcg aatatggtcg atatgaaaac
     4801 tacgcatacc tccctcccct ttgccggaca tacgctgcat tttgttgagt tcgatccggc
     4861 gaatttttgt gagcaggatt tactctggct gccgcactac gcacaactgc aacacgctgg
     4921 acgtaaacgt aaaacagagc atttagccgg acggatcgct gctgtttatg ctttgcggga
     4981 atatggctat aaatgtgtgc ccgcaatcgg cgagctacgc caacctgtct ggcctgcgga
     5041 ggtatacggc agtattagcc actgtgggac tacggcatta gccgtggtat ctcgtcaacc
     5101 gattggcatt gatatagaag aaattttttc tgtacaaacc gcaagagaat tgacagacaa
     5161 cattattaca ccagcggaac acgagcgact cgcagactgc ggtttagcct tttctctggc
     5221 gctgacactg gcattttccg ccaaagagag cgcatttaag gcaagtgaga tccaaactga
     5281 tgcaggtttt ctggactatc agataattag ctggaataaa cagcaggtca tcattcatcg
     5341 tgagaatgag atgtttgctg tgcactggca gataaaagaa aagatagtca taacgctgtg
     5401 ccaacacgat taattgacaa catctggtac gattcgcccg cagccatcac tgaccacggg
     5461 cgaaagtgta aagcaggtgc cttaccatcc tgacctgaca accggatatg cggggattgc
     5521 tccccgcaca acggctactt cttcggttcg taagcgagaa cagccttaaa ctcaatatta
     5581 cgttccttta cggtgaactc acacagcgaa tctccgacca gaagcgtaaa tcccagcacc
     5641 gttaaacaca gcactatgac tgccgcaagg gcatatttcg tcagcatgtt tatattgcct
     5701 ccatgcgaag aagggactac catccaaatt gttcgggttt ggagtggcag ccccagggtt
     5761 gatagaaata tctcctgggg cttcatcttt ccagacctca gagtaaacct gaaaccggaa
     5821 agcttcaggc acccgccgtt atcttaccct tcctcaacaa aatagctgtt tatttataca
     5881 gtgagtcctg tttcggactt ctcactcctc atttaatcaa ctgaagctga acccgttttt
     5941 ttctgcatga ctcaatcgtc aaaattcttc gccaccagtc agcactgcca taacttgtca
     6001 cattgcaaaa gtagccaaat tgcttcaggc accatcagtt caccatcacc aaaaaaaaga
     6061 aacattccag aacttccgca tctcgcccag ccgctccgac ttctatactg aatagaaaac
     6121 gccaacataa gagaaaccta tgccattacc cgattttcat gtttctgaac cttttaccct
     6181 cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa gccaggactc
     6241 ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa agcacgatat
     6301 caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc aggctgccgg
     6361 gcagttttca gcgatgcaga aagtcgtatt gcaggcagcc acagaccatc atctggaaat
     6421 ttgcggcggt ggcacgcacc cgtttcagaa atggcagcgt caggaggtat gcgataacga
     6481 acgctatcaa cgcacgctgg aaaactttgg ttatctcatt cagcaggcga ccgtttttgg
     6541 tcagcatgtc catgttggct gcgccagtgg cgatgacgcc atttatttgc tgcacggctt
     6601 gtcacgattt gtgccgcact ttatcgccct ttccgccgcg tcgccatata tgcagggaac
     6661 ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcctg ataatggccc
     6721 gatgccgtgg gtcagtaact ggcaacaatt tgaagcccta tttcgctgtt tgagttacac
     6781 cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc ctcattttgg
     6841 cacggtggag gttcgggtga tggatacccc gttaaccctt agccacgcgg taaatatggc
     6901 gggattaatt caggccaccg cccactggtt actgacggaa cgcccgttca aacatcagga
     6961 gaaagattac ctgctgtata aattcaaccg tttccaggcc tgtcgctatg ggctggaagg
     7021 cgtcattacc gatccgcaca ctggtgatcg tcgaccgcta acggaagata ccttgcgatt
     7081 gctggaaaaa atcgcccctt ccgcacataa aattggcgca tcgagcgcaa ttgaagcact
     7141 gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt tcgtcgccga
     7201 tggcggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggccg gtgactaagt
     7261 cgggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac atttaacaat
     7321 gttatgaaac accctttaga aaccttgacc accgcagcag gcattttgct gatggctttc
     7381 ctctcttgcc tgctgcttcc cgcccccgca ttggggctga cgctggcaca aaaactggtg
     7441 agcacgttcc atctgatgga tcttagtcag ctttacactt tattgttttg tctgtggttt
     7501 ttagtgctgg gcgctattga gtattttgtt ctgcgcttta tctggcgacg ctggttctcg
     7561 ctggcggatt aaacgtcgat tatcgccgta cttcgcggca tactttgctt attctccttc
     7621 gcctaaagga acgtttatgg ataagcaatc actgcacgaa acggcgaaac gcctggccct
     7681 tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg tttttaaaat
     7741 tggcggcaag atttttatgc tgtcgtcgga gctacgcggc gtccccttta tcaatctgaa
     7801 gtccgatcca caaaaatccc tgttaaatca gcaaatatac ccaagcatta agccagggta
     7861 tcacatgaat aaaaagcact ggatttcggt gtatcccagc gaggaaatct ccgaagcgtt
     7921 acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg ctaaacgcga
     7981 tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag cgcatttttc tcgcttacca
     8041 tttctcgttg aaccttgtaa tctgctggca cgcaaaatta ctttcacatg gagtctttat
     8101 ggatatcatt tatgtcgcct taaagcgtca ttccactaag gcatttgatg ccagcaaaaa
     8161 acttaccccg gaacaggccg agcagatcaa aactctcctg caatacagcc catccagcac
     8221 caactcccag ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag cgcgtgttgc
     8281 caaatccgct gccggtaatt acgtgttcaa cgaacgtaaa atacttgatg cctcgcacgt
     8341 cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg ttgttgacca
     8401 ggaagatgcc gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg ataaaggtcg
     8461 caagttcttc gccgatatgc accgtaaaga tctgcatgat gatgcagagt ggatggcaaa
     8521 acaggtttat ctcaacgtcg gtaacttcct gctcggcgtg gcggcgctgg gtctggacgc
     8581 ggtacccatc gaaggttttg acgccgccat cctcgatgca gagtttggtc tgaaagagaa
     8641 aggctacacc agtctggtgg ttgttccggt aggtcatcac agcgttgaag attttaacgc
     8701 tacgctgccg aaatcacgtc tgccgcaaaa catcacctta accgaagtgt aattctctct
     8761 tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata accctgtttc
     8821 agccgtcatc ataggctgct gttgtataaa ggagacgtta tgcaggattt aatatcccag
     8881 gttgaagatt tagcgggtat tgagatcgat cacaccacct cgatggtgat gattttcggt
     8941 attatttttc tgaccgccgt cgtggtgcat attattttgc attgggtggt actgcggacc
     9001 ttcgaaaaac gtgccatcgc cagttcacgg ctttggttgc aaatcattac ccagaataaa
     9061 ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca ggcggtattc
     9121 tggctgcaaa aaggcaccga agcggcagat attctgacta cctgcgcgca gttgtggatc
     9181 atgatgtatg cgctgctttc agtcttctcg ttgctggatg ttattttgaa tctggcgcag
     9241 aaattcccgg cagcatccca gttaccgctg aaagggatat ttcagggggt taaactgatc
     9301 ggcgcgattc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc gccagcgatt
     9361 ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa agatccgatt
     9421 cttggtctgg tggcaggtat tcagctttcc gcgaacgata tgctgaaact gggcgattgg
     9481 ctggagatgc cgaaatacgg cgcggatggc gcggtgatcg atattgggtt aaccaccgtc
     9541 aaggtgcgta actgggacaa taccattacc actattccca cctggtctct ggtttctgac
     9601 tcctttaaaa actggagcgg gatgtcagca tccggcgggc gacgtattaa gcgcagtatc
     9661 agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg tctgaataaa
     9721 gcgcatttgt taaagcctta tttaaccagc cgccatcagg aaattaatga gtggaatcgc
     9781 cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat tggaaccttt
     9841 cgtgcctatc tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga tatgacttta
     9901 atggtacgcc aactggctcc aggtgataac ggtttaccgc tcgagatcta tgcgtttacc
     9961 aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga tcacatattt
    10021 gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa tgatattcgc
    10081 tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa tggatgagag
    10141 cggacatcgt tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc gttttaaatg
    10201 ccacaaacag gaatacaatc cacaccggca gcaggatcgc tgacaagcgc atatcatcca
    10261 tcgtgcacat cagcagcaaa atcatgccga ggaaggcaat gcaaagatag ttgccgaacg
    10321 gataaagcag cgccttaaac tgtgtttcac gtccctgacg tcgcatcgct gcacgaaaac
    10381 gcagatgcgc caggcagatc ataatccagt tcaacagcag cgttgctacc accagcgcca
    10441 tcagcagacc aaacgctttt tgcggcagca gatagttgat taataccacc agcgaagtga
    10501 tcgctccgga aagcatcagc gaattaatcg gcacaccgcg acggctgacg cgagtcaaaa
    10561 acttcggcgc attaccctgc acagaaaggc caaacagcat gcggctgtta gagtaaaccc
    10621 cgctgttata cactgacagc gatgctacca gaatgacgaa gttcagcgca gaagctacca
    10681 cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat ttcacttcca
    10741 cccacggata gagcgccagt aaaaccacca gtgaaccgat gtaaaacagc aggatgcgat
    10801 acaccacctg atttaccgct tttggaatgc ttttttccgg atcgcgcgct tcagcggcag
    10861 taatcccaat cagctccaga ccgccgaagg agaacataat taccgccagc gacaaaatca
    10921 gcccattcca gccggtggca aagaaaccac cgtagcgcca gaggttgtcg atactggctt
    10981 tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata ccgatgatcg
    11041 ccagcacttt aatcagcgca aaccagaact cggtttcacc atataagcgc acgttcacca
    11101 ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt ggaacatccg
    11161 ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccatcccc accagcacga
    11221 acatcaccca gtagttccag ccagagagga agcccgcgaa cggtccccag tatttatagg
    11281 caaagtgggc aaacgaaccg gataccggct cttcgaccac catctcgccg agctggcgca
    11341 taatcaggaa agcgatgatc ccggcgacgc cgtagcccag caatacagcc ggacccgcca
    11401 tctgaatcgc cgggccaatg ccaagaaaca gaccagtacc aattgcgcca cccagcgcaa
    11461 tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga ttcgacgcag
    11521 tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct tttttgttga
    11581 ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac ggttaaacgg
    11641 ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct tatacgccgc
    11701 cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca tcggcgcggc
    11761 aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca gcagaccggc
    11821 gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc aggaccgcgc cgtgatataa
    11881 cgcctcatcc agcttctgct cgctgaatgt ttgtggatta ttcaacgacg gctcggcctc
    11941 tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcga ccccggcgag
    12001 ggcgataaag ccagtgcccg tcgccacgga aagatgaaag cccatccacc acagcaacca
    12061 gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt cgcccacccg
    12121 acggaacgcc agatacaaca gcacgaagat aatcatcaac gtcatcggca ccatgagttt
    12181 aagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca cgctggtgcc
    12241 cggttttaac tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca ccgacaccat
    12301 gtcacgatcg cgggcatcga tataaatcca gctcgtcggg cgcgcattct cggttttcag
    12361 catcgacggt ccggtagaga ctttaatgtc ggccacgtct gccagggtga tttgctgctt
    12421 catcggcgtc aggatcggca gctggcgcag tgcctgcgga ctatcgcgcc agctttgcgg
    12481 ataacgcaga ttaattggat aacgggcaat cccttccacc gtttcgccaa ccatcgcccc
    12541 gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt aacgtgcggc
    12601 tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagccgct cggcaagcgc
    12661 agaagctacg cctggcaccg ttcgcgctac ttcttcaatt tgctcagcca tcgcgtcgat
    12721 atccgccagc acagtgccgg aaactttaat gccgatgggg cttttaatgc cggttgagag
    12781 catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg gcagccgcac
    12841 ggtgttatcc agttcctcaa tgattttgtc catcgtcatg cctggccgcc actgctcctg
    12901 cggcttaagc tggatggtcg tttctaccat ctccagcgga gcagaatcgg tggcggtttc
    12961 cgctttcccg gttttgccaa atacccgcgc cacttcaggt acgctcataa ttagcttgtc
    13021 ggttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg tcgatggcat
    13081 atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt tattgagcgg
    13141 ccagagaacc gtcagcaccg aaagcgccgc caccagcagc gtggttttcg gccagtgcag
    13201 tactttcagc aacagcggat gataaacacg aatcaaaaag cgattgagcg ggttactgct
    13261 ttccggcgga attttgccac ggatccagta gcccatcagg atcgggatca ctacgatcgc
    13321 cagcagcgcc gcacccgcca tcgcatacgt tttggtgaac gccaacgggc caaacagacg
    13381 cccttcctgc ccttccaggg tgaagatcgg gataaacgac aacgtgataa tcagcagact
    13441 gataaatagc gccggcccca cttcaacaga cgcatcggtg atcacctgcc agcgcgtttt
    13501 attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt tatgcgcatt
    13561 ctcgatcatg acgatagcag catcgaccat cgccccgacg gcaatcgcaa tgccacccaa
    13621 cgacataata ttggcattca gtccctggaa gtgcatgaca ataaaagcaa tacacaaccc
    13681 cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa acagcgcaca
    13741 gactaccgcc accacaataa actcttccag caacttgccg ctgaggttgt cgatggcgcg
    13801 gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acaccttccg gcagactact
    13861 tttcagcgtt tccagtttgt ccttcacggc ggcgatcact tctcgggcgt ttttgccgga
    13921 tcgcaggatc accaccccgc ccgcaacttc gccttcgccg ttcagttcgg caatgccccg
    13981 gcgcatctcc gggccaacct ggaccttcgc aacatcacgc agataaacgg gcacgccatt
    14041 ttcactggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat agccgctggc
    14101 gcgcaccata tattccgctt ccgccagttc gatcgacgaa ccgcccgctt cctggtttga
    14161 agcatccagc gcatttttca cttcgccgag actgatgcca tactgcgcca ggcgctgggg
    14221 atcgataacc acctgatact ctttcaccac accgcctacc gacgccactt ccgcaacgtc
    14281 aggaatggtt ttcagctcat atttgagaaa ccaatcctgc aatgagcgta aatcggcaag
    14341 atcgtgctta ccgctgcgat ccaccaatgc atattcatag atccagccaa cacccgtggc
    14401 atctggtccc agctcggcgc tgactcccgc aggcagctta ccctgtacct ggttgagata
    14461 ctccagcacc cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa tgacatacac
    14521 ataagagtcg ccaaactgcg agaaaccacg cacagtcttc gcgccaggca ccgacagcat
    14581 ggtggtagtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct gaccgggata
    14641 gctggtttta ataatcacct gcacatcgga gagatccggc agcgcatcca ctggcgtatt
    14701 aatgatggtc caggtgcccc agatgctcag aaacaacgcg cccatcagca ccaaaaaacg
    14761 gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca atgcgcatgg
    14821 gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc ggaatcaatc
    14881 aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagcg taatgcggtg
    14941 acgccttgcg atgcctggaa aacagcaacg cgtttcggta caaagcgccc gtcggcatca
    15001 acggtaatca cccgctgttc gctgccggta tcaatcagcg cttgtgacgg aatgagcagc
    15061 atcggttcgc tggcggtgtt gagttgcaac caggcgttca tgcccggttt tagcgcctcg
    15121 tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc cacgccaggt
    15181 agcagcgtcc atttgcggat ggtgagtgtt ttatccggtc gcgccggaac ggtgagggta
    15241 aactgcgagg catctttcac cagccaggcg atggactccg ggatcgcagc agtgacccac
    15301 accgggtcca taccctgaat tttcgcgacc acgttatctt tggcgatatt cattcccgcg
    15361 cgcagatcaa acgcggtgat cacgccatca atgggcgctt tgagcgtaaa gcgagtctgg
    15421 attttttgcg tggcgatcag gcggcgaata tccgcctccg gcattcccgc cagtcgcagc
    15481 cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag cagtaaatac
    15541 tcactctgcg cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt gcccttttgt
    15601 actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc gcgagcctgc
    15661 acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg ggcaaaagtc
    15721 agcggtccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg agtcgggtca
    15781 atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag atccatatcc
    15841 ataaacggcg atttacctgg tttatcgaac cgcgtattgg gatacattgg gtcgtaccag
    15901 aataagattt tacgttctgc ggtcgacgtt ttttctgcag gcggttccgc ctttgcaacc
    15961 caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat aataagcgcg
    16021 atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg taataaagaa
    16081 aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat ttcactcatt
    16141 ttcgtctgcg gggtgatggt aaagcgcatg gtcatctccg gccagttcac agcagcaatc
    16201 ggatcgtgat ggatggtgat ttttttgctt tccagatcaa taccctttac ctcgccagtg
    16261 gcactaataa cctgtggttg tgcttcgctc atggtttcat gatgatgttc gttagcctgg
    16321 gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc ttttttcatt
    16381 tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg gtatacaaag
    16441 aaatttcgtt aacctgacgg gcataattca ggtcgagtaa agtttgtcgg gttgcaaata
    16501 aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga tataatgccc
    16561 gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg ctgatttgat
    16621 cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg ttctggattt
    16681 tctgttcata attcactacc gactgctgct ggcgaatttc ggcgatatcc agattggcct
    16741 ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc cacatcccgc
    16801 tgctagcgtt aaataatgaa gacagatcgc tgctggcggt tgatattccg ctggtcaggc
    16861 tgatagacgg gaaaaatgcc gcacgcgcag cgccaatatt ggcattagcc gccattaacg
    16921 cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac aatcccgccg
    16981 gtaatttaac gctttgcagg ctgtcgctgt ttactgtctg cgcttgcggc agcttgccgt
    17041 agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc tgacgtttag
    17101 cgatgtcgct gcgggtactt tcaatcaccc cgcgagcctg ttccagcgcc agaacattac
    17161 tgctacctgt caacagttgt ttttcgacaa acgcatatga ctgctgataa ttacgcagcg
    17221 tttcttcggc tatttgcaat tgcgcatacg ctagctgctg attgaaataa ctttgcgcga
    17281 cattagaaac cagcaaaata tgcaccgcgc gctgggcttc ctcagtggct aaataatttt
    17341 ggcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgagatca aagctggcgt
    17401 taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta ccgctccagc
    17461 tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga cgatattgcg
    17521 cccgcgcttc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc accagcgcct
    17581 cgctaatcag cgtctttacc tgattatcaa caaaaaaggt gcgccagccc acgttctggt
    17641 agttatctgc tgcgttaacc aagccgttct ggctgagtga gaactgctgc ggcacgggca
    17701 ttgccggacg ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg gccacacaaa
    17761 atggcagaag tttacaagga gacataggct cataatttct ggtgatttta taccgccaac
    17821 tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt cattttgcca
    17881 ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat gcggaggaaa
    17941 aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga ccaaagggtt
    18001 aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ttgaatggct accatctggc
    18061 gatgaccggt gattatgatc tgataatcct cgatattatg ctgccggacg tgaacggctg
    18121 ggatatcgtg cgcatgttgc gttccgccaa taaagggatg ccgattctgt tgcttaccgc
    18181 gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggccgatg actatctggt
    18241 gaagccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc ggcgcggggc
    18301 ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc tcgtcagccg
    18361 caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagagttta ctctgctgga
    18421 gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct cgcaggtgtg
    18481 ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc ggctacgcgg
    18541 caaaatcgac aacgactttg agccgaagct gattcagacc gtgcgcggcg tgggctatat
    18601 gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg gcaacccgtc
    18661 tgaccttttt tatcagcctg gccaccatcg cggcgttttt cgcctttgca tggatcatga
    18721 tccactcagt gaaagtgcat tttgccgagc aggatattaa tgatttaaaa gaaattagcg
    18781 ccacccttga acgggtactt aatcatcctg acgagacgca agcccgacgc ttaatgacgc
    18841 tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat agccacggca
    18901 aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt gacgccatac
    18961 ccgataaaga cgctcagggt ggcgaggtgt atctcctttc cggcccgacg attatgatgc
    19021 caggccacgg tcacgggcat atggaacaca gcaactggcg gatgattaac ttgccggttg
    19081 gcccgttggt ggacggcaaa ccgatttata cgctctacat cgcgctttcg atcgattttc
    19141 atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatct gtcatcagca
    19201 tcctgatcgt ctttatcgtg ctattggcgg tacataaagg tcacgcgccg atccgcagcg
    19261 tcagccgtca aatccagaat atcacctcga aagatctcga cgttcgcctt gacccgcaga
    19321 ccgtgcccat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc gagcgtattg
    19381 aggatgtctt tacccgccag tccaatttct cagcggatat cgctcacgaa attcgcacgc
    19441 cgattaccaa tctcatcacg caaacggaaa tcgccctcag ccagtcgcgc agccagaagg
    19501 aactggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg aaaatggtca
    19561 gcgatatgct atttctcgct caggccgata acaaccagct aatccccgaa aagaaaatgc
    19621 tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgtta gcggaagatc
    19681 gcggcgtgga gttgcggttt gttggcgaca aatgtcaggt tgcgggcgat ccgctgatgc
    19741 tgcgtcgggc gttaagcaac ctgctttcta atgccctgcg ttatacgcca cccagtgagg
    19801 ctattgtagt gcgctgccag acggtcaatc atcaggtgca agtttccgtc gaaaaccccg
    19861 gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctat cgcgttgacc
    19921 cttcccgcca gcgaaaaggt gaaggtagcg gtattgggct ggcgatagtg aaatcgattg
    19981 ttgtcgcgca taaaggcacg gttgcggtaa cgtcagatgc gcgggggaca aggtttgtga
    20041 ttatgctgcc ggagagagag tgattattaa gttcaaattc agggcgtggt gcatcacctc
    20101 ctggtattgt tgatcaacaa attagagaaa aacttattaa acatatgaaa tatcgaaggc
    20161 ttttattttt caaagtttca cgagatgttt gtatatgaaa cttagttaaa tctgtgtttc
    20221 ttccaattgt agatcacaat gccctttcta aattttcatc gttata
//
LOCUS       DAOFQD010000082        20231 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.082, whole genome shotgun sequence.
ACCESSION   DAOFQD010000082 DAOFQD010000000
VERSION     DAOFQD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.082"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(48..2117)
                     /gene="glyS"
                     /locus_tag="QBF46_003098"
     CDS             complement(48..2117)
                     /gene="glyS"
                     /locus_tag="QBF46_003098"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDQ6675248.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2127..3038)
                     /gene="glyQ"
                     /locus_tag="QBF46_003099"
     CDS             complement(2127..3038)
                     /gene="glyQ"
                     /locus_tag="QBF46_003099"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDQ6675249.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3133..3432)
                     /locus_tag="QBF46_003100"
     CDS             complement(3133..3432)
                     /locus_tag="QBF46_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDQ6675250.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3607..4602
                     /gene="wecH"
                     /locus_tag="QBF46_003101"
     CDS             3607..4602
                     /gene="wecH"
                     /locus_tag="QBF46_003101"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDQ6675251.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4644..5081)
                     /locus_tag="QBF46_003102"
     CDS             complement(4644..5081)
                     /locus_tag="QBF46_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDQ6675252.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5127..5468)
                     /locus_tag="QBF46_003103"
     CDS             complement(5127..5468)
                     /locus_tag="QBF46_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675253.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFTHICQLVALITIGLLFIGVLNAPINTYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            complement(5637..7091)
                     /gene="xylB"
                     /locus_tag="QBF46_003104"
     CDS             complement(5637..7091)
                     /gene="xylB"
                     /locus_tag="QBF46_003104"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDQ6675254.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAVGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7163..8485)
                     /gene="xylA"
                     /locus_tag="QBF46_003105"
     CDS             complement(7163..8485)
                     /gene="xylA"
                     /locus_tag="QBF46_003105"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDQ6675255.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8851..9843
                     /gene="xylF"
                     /locus_tag="QBF46_003106"
     CDS             8851..9843
                     /gene="xylF"
                     /locus_tag="QBF46_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6675256.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9921..11462
                     /gene="xylG"
                     /locus_tag="QBF46_003107"
     CDS             9921..11462
                     /gene="xylG"
                     /locus_tag="QBF46_003107"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDQ6675257.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIHDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11440..12621
                     /gene="xylH"
                     /locus_tag="QBF46_003108"
     CDS             11440..12621
                     /gene="xylH"
                     /locus_tag="QBF46_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDQ6675258.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12699..13877
                     /gene="xylR"
                     /locus_tag="QBF46_003109"
     CDS             12699..13877
                     /gene="xylR"
                     /locus_tag="QBF46_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDQ6675259.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(13985..14809)
                     /locus_tag="QBF46_003110"
     CDS             complement(13985..14809)
                     /locus_tag="QBF46_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDQ6675260.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15129..17159
                     /gene="malS"
                     /locus_tag="QBF46_003111"
     CDS             15129..17159
                     /gene="malS"
                     /locus_tag="QBF46_003111"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDQ6675261.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYERHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17337..18590
                     /gene="avtA"
                     /locus_tag="QBF46_003112"
     CDS             17337..18590
                     /gene="avtA"
                     /locus_tag="QBF46_003112"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDQ6675262.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18741..19214)
                     /locus_tag="QBF46_003113"
     CDS             complement(18741..19214)
                     /locus_tag="QBF46_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDQ6675263.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19316..20164)
                     /gene="yiaJ"
                     /locus_tag="QBF46_003114"
     CDS             complement(19316..20164)
                     /gene="yiaJ"
                     /locus_tag="QBF46_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDQ6675264.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
BASE COUNT         5080 a         5174 c         4959 g         5018 t
ORIGIN      
        1 cgataaaaaa agcctgccag atggcaggct atttaataac ggcgttatta ttgcaacagc
       61 gaaatatccg caacgcgcag gaacagttcg cgcagtttct ccagcatggt cagacggttg
      121 atacgcaatt ctttgtcatc aaccatgacc atcactttat cgaagaaggc atcaaccggt
      181 tcacgcagct cagccagttc gaccagcgca tcctggtaac gaccttcagc aaagtacggc
      241 tccagcttgt cacgtagcac cacaacctgc atcgccagtt taatttcttc cggctctttc
      301 agggtagagg cgttcacacg gtcgctcagc acttcgtcag atttcgccag aatgttagat
      361 acacgcttgt tcgccgccgc cagtgctgca gctgcatcca gggtacggaa gtgcgatact
      421 gctttcatac gggcatcgaa atctgccgga cgagtcggac gacgcgccag taccgcctgg
      481 atggtatcaa cggtataacc ttcgtcctga taccaggcgc ggaagcgacc gagcataaag
      541 tcgataacat catcaactac gttggcatta gtcagcttat cgccatacag acgcaccgct
      601 tcttcggtca gcgtttgcag atcaaggttg aggttcttct caacgataat acgcagcacg
      661 ccaagcgcgg cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg ctgaccgata
      721 ccgaagatac ccgccagagt atccatcttg tcagcaatcg ccagcgcaca ggctaccggg
      781 ttagacggca ggtcatcacc ggcaaagcgc ggctgatact gctcattcag cgccaccgcg
      841 acatcctccg cttcgccatc gtgacgcgca tagtgcatcc ccataacgcc ctgggtgtcg
      901 gtgaactcga agaccatgtt ggtcatcagg tcacacttag acagcaggcc cgcacgggtt
      961 gcgtggttaa cgtcagcgcc aatctgttca gcaatccagc cagccagcgc ctggatgcgg
     1021 tcagttttgt cacgcagcgt acccagctgt tgctggaaca gcacggtttg caggcgcggc
     1081 aggttatctt ccagacgttt tttacggtcg gtgttgaaga agaactcggc atccgccaga
     1141 cgcggacgaa cgactttctc gttaccggag ataatctgct gcggatcttt cgattcgatg
     1201 ttggcaacga agataaagtt cggcagcagt ttgccgtcgt tcgcatacac cgggaagtat
     1261 ttctggtcac ctttcatggt gtaaaccagc gcttcagccg gcaccgcgag gaatttctct
     1321 tcgaattttg cggtcagaac gaccggccac tccaccagcg aagccacttc ttccagcagg
     1381 ctttcgctta agtcagcgtt accgccaatc ttacgcgctg cttcttcggc atcggcttta
     1441 atcttcgctt tacgttcttc gtaatcggcg atgactttcc cgcgctcacg cagaatttcc
     1501 ggatactgat cggcgttgtc gatggtgaat tccggttcgc ccataaagcg gtggccgcga
     1561 atcacgcgat cggactgaat gcccagaatg gttgccggaa tgactttgtc gcccagcagc
     1621 agggtcacgg tgtgcaccgg acgcacgaag tgcacgtcgc ttgcgcccca acgcatcagt
     1681 ttcgggatcg gcagtttcgc cagagaagtc gcaaccatat tcggcagcag tgcttcggtg
     1741 ctttcgccct tcacatgggc gcgatacagc agccattcgc ctttatcggt agtcagacgc
     1801 tcagcctggt caacggtaat accgcaacca cgcgcccaac cttctgccgc tttgctcggt
     1861 ttgccttcag cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat ttcacgatcc
     1921 ggttgcgctt ccgccaggtt agctactttc agcgccagac gacgcggagc agcaaaccat
     1981 tgaacggtgc cgtgtgcgag gccagcgtta tccagctccg cagtaaaatt cgcagcaaag
     2041 gactcagcca ggctgcgcag tgcttttggt ggcagctctt cagtgccgat ttccaccaga
     2101 aaagttttct cagacatagc cgcctcttac ttatctttgt tgcacatcgg gaagccgagg
     2161 gcttcacggg aagcgtagta tgcttctgcc actgctttgg tcagggtgcg aatgcgcaga
     2221 atgtagcgct gacgctcggt gacggagatg gctttacgcg catccagcag gttgaagctg
     2281 tgggcggctt tcagaatacg ctcgtaggct ggcagcggca gcggattttc cagcgccagc
     2341 agctgctgcg cttctttctc gtactgctca aagcaggtga acaggaagtc cacatccgcg
     2401 tattcgaagt tgtaagtgga ctgctccact tcgttctgat ggaacacgtc gccgtaggtg
     2461 gttttaccca gcgggccgtc gctccagacc aggtcgtaaa cgctgtctac gccctgaatg
     2521 tacatggcca gacgttccag accgtaggtg atctcgccgg taaccggttt acactccaga
     2581 ccaccaacct gctggaagta agtgaactgc gtcacttcca tgccgtttag ccacacttcc
     2641 cagcccagtc cccaggcacc cagagtcggg ttttcccagt tatcttccac gaaacggatg
     2701 tcgtgaatag tcgggtccat gcccagctct ttcagagaac cgaggtacag ctcctgaata
     2761 ttgtccggcg atggcttaat gaccacctgg aactgatagt agtgctgtaa acggttgggg
     2821 ttttcgccgt agcgaccatc ggtcgggcga cgagaaggct gaacataagc agccgccatc
     2881 ggttctggcc ccagcgcgcg cagacaggtc attgggtgag aggttcccgc gccgacttcc
     2941 atgtccaatg gttgaacaat ggtgcagccc tggcgagccc agtaatcctg taaggtcagg
     3001 atcaagccct ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc tggatacgtg
     3061 tggatttaaa ggaagggatc agtatacccg ctggatggaa gatatacagt acgaaacggg
     3121 aaaaagcagg gcttaacgca tggaaagatg caaaaaatgg ccatccgcgt caaaagaaca
     3181 gacaaactgc tctttacgga aggtataacc gcgcatttca tagcttccct ggaactgttc
     3241 gaaggcggtg acgtcgattt tttgctcgcc ggtgttataa cgttttgccg cctgatcctt
     3301 gcataacgct tccatattca acgaacgcag aggatcaacc tttacccgtt gtgctttctg
     3361 cacgggtgac ggtgtagaac aacccactag caccataagc gccattgccg gaaagaaagc
     3421 gttcatcatc attaaattac cgtctggttt gcaggtgagt cttattattt atatcggtat
     3481 agtggctgat atcttacgaa tttccttaag cctctataag ctgtagtgat aaaactcaga
     3541 ttattccagt gcgccaacca aatgatttat cggtcagact ttttttaacc agatacagag
     3601 acacagatgc agcccaaaat ttactggatt gataacctgc gagggatagc gtgtttaatg
     3661 gtggtgatga ttcacaccac tacctggtat gtgaccaatg ctcatagtgt tagccccgtc
     3721 acatgggata tcgccaatgt tctgaattct gcctctcgtg tcagcgtgcc gctatttttc
     3781 atgatttccg gctatctctt ttttggcgaa cgcagcgccc agccgcgcca tttcttgcgt
     3841 atcggcttat gtctgatttt ttatagcgca atcgcactgc tctacattgc gctgtttacc
     3901 tccatcaata tggagttagc gctgaaaaac ctgctgcaaa agccagtgtt ttaccacttg
     3961 tggtttttct tcgcgattgc ggtgatttat ctggtttcac cgctgattca ggtgaagaac
     4021 gtcggcggaa aaatgttgct ggtgctaatg gcggtgattg gcattatcgc taacccaaac
     4081 acagtgccgc agaaaattga cggttttgaa tggctgccaa ttaacttata tatcaatggc
     4141 gatacttttt actacattct gtatggcatg ttgggccgcg ctatagggat gatggacaca
     4201 cagcataaag cactgtcgtg ggtgagcgcc gcgctgtttg cgacgggggt ttttattatc
     4261 tctcgcggga cattatatga attgcagtgg cgcggaaatt ttgccgatac ctggtatctt
     4321 tactgtgggc cgatggtttt tatctgcgca atcgcgctat tgactctggt taaaaacacg
     4381 ctggatacgc gtaccattcg cggacttggc ttaatctccc gccattcatt gggtatatac
     4441 ggattccacg ccttgattat ccatgcgctg cgcacccggg gaattgagct taaaaattgg
     4501 ccaatactgg atattatttg gatcttttgc gcgacgttgg cagcgagttt gttactttct
     4561 atgctggtac aacgaatcga cagaaacaga ttagtgagtt aagtaaaagc ccggtcacat
     4621 tggactgacc gggcttacgt gagttattcg ctgtattctt cctgggtaac ctgtgtttct
     4681 tttggtgggt ttattccggc atcacgaata tttttctgca ccgctactgc accaaacaag
     4741 cttaagaaga aagccagtcc ataaaaacct ttttcgctga gtaataatgt cgcattccac
     4801 agacctacca tcagtaatgc aacagagata ataaagacag tcaggcaggt catataataa
     4861 atggaagtgg tcggtatgcc ttcatacttg tcccgaacgg tcttttgata agacgccgca
     4921 gaaaacagtc ctaataccag tacggcaaaa taatatcctt tttcatttaa ctgcatctct
     4981 gcattccata gccctaacag ataggtaacg ataccaccaa cgagagctat ccatgacaca
     5041 atactaaagg ccggtgaata ggttgatatt ttgttgtcca tcacagtatt cctttcattc
     5101 ctgaatatgt aagagcttta tgttgctcat gccgagcgaa acaaacgcat ttgaccaaac
     5161 aagcagacaa aaaaggcgat gggatagatc accatttcat aagtattgat aggtgcgttt
     5221 aaaacaccga taaacaagag accgatagtg attaacgcaa ctaactggca gatatgggta
     5281 aaaaaatcat ccagttgccc gagtttttct gcgcgaagat atgcatagtt gccaaaagtt
     5341 gctgtcatta acacgccaag aaaatagcct tttccactta acaatgggca tgagatccat
     5401 agcccaacca gataaaccag cgccccgaca acaaataata gttttgccac agtctttgag
     5461 gttttcataa cgatctccat atctaccagc gatacattac gagtaaccaa cgaaagacaa
     5521 aactgaaaaa tgccattaac aaatgatttt cagaataaat tcatactaaa tattaattaa
     5581 ttactgagat atatagatgt gaattatccc ccacccggtc aggcagggga taacgtttac
     5641 gccattaatg gcagaagttg ctgatagagg cgacggaacg tttctcgtcg tggctgataa
     5701 gcggcataac gctgcgcatc tggtagatgc gactgttcta acggtagttg cggcaacaat
     5761 tcaatgagcg atttctctgg attcgccgcg atctgcgcca gccttgctgc gcccagtgct
     5821 ggccccacat ccccccccgt acggtaatcg agctgctgac cgctgatatc cgccagcatc
     5881 tgacgccagt actcactacg cgccccgccc ccaatcaacg taacactttg cggtttaata
     5941 ccgcaggcat gcacgacatc catgccatct gccagcgcat aacccacgcc ttccagcact
     6001 gctcgcgcca gttcattggg gccatgttga tgagtcaaac caaagaaaac ccccttcgcc
     6061 tggggattat tgtgtggcgt acgctcgccg gaaagataag gcagaaacca aactggctcg
     6121 gcactttcat cagcctgttg agctgcagcg attaaagctg ggacattgct caggccggtt
     6181 aatttcgcgg cccaatccag acacgacgct gcactcagca tcacagacat taaatgccaa
     6241 cgttgcggta gcgcatggca aaagctatgt acggcgcttt ctggcttgct taagaaccct
     6301 tcgctgacag caaaatagac ccccgacgtc cccagcgata acattgcctg attagcatca
     6361 accattccca caccaactgc accaactgca ttgtcgccac cgcctgcgac aactggcacc
     6421 gtcgccatac cccacgcttt cgcaacttca ggtaacaaag caccagtaat ttcgctgcct
     6481 tcgtataatg cgggcatctg gtcacgagat aagtcgcaag cctgcagcat gacgtcactc
     6541 cagtcacgct ttgcgacatc cagccacatg gtgccagctg cgtcagacat atcgctggca
     6601 aactcccccg tcatacgcag acgcaagtaa tctttcggta ataatacttt gtcgatttga
     6661 cggaatatct ccggctcatg ccgctgaacc catagcaatt taggcgcagt aaatccgggc
     6721 atcatcaggt tgccggtaat cactcgtgat tgcggaactc tcgcttccag caaagtgcac
     6781 tcttgcgcac agcgcccgtc gttccacaaa atggcagggc gcaatacccg ttgttgagca
     6841 tccagtaagg ttgctccatg catctggccg gcaataccca atgctttaac gtcctgcaga
     6901 gaatgctgat cgcccagagc tttcattgcg cgatcagttg cctgccacca ctgttccggg
     6961 tcttgttccg accagagtgg atgcgggcgc gaaacggtca gcttttccgt ttgcgaagca
     7021 accacctcac cctgctcgtt gagcaaaata acttttacgc ccgaggtgcc aagatctatc
     7081 ccgatataca tatcgatcgt tccttaaaaa aatgcccggt atcgctaccg ataaccgggc
     7141 caacggactg cacagttagc cgttatttgt cgaacagata atgatttacc agattttcca
     7201 gttgctcctg gcgaccactc tgatgcaccg gagacaaatt atgttcctga gcatatttgg
     7261 ctaaatctgc cagtgacatt tggcctttca ggatttgctg gcccaattcg ctattccagc
     7321 cggaataacg ctgcgcgatg cgtttatcca gctcgccatc ttcaatcatg cgcgctgcaa
     7381 ttttcagcgc cagtgccatc gtatccatcg cgccgatatg accgtaaaac agatcatatt
     7441 tatcagtact ttgacgacgt actttggcat cgaagttcag accaccggtg gtgaaaccgc
     7501 ctgctttgag aatttcatac atcaccagcg cattctcttc cacactgttc gggaactggt
     7561 cggtgtccca gcccagttgc gcatcgccac ggttggcgtc gacagaaccg aacaggccaa
     7621 gcgcaatggc ggtggctatt tcatgatgga aagagtgacc tgccagcgtc gcgtggttag
     7681 cttcaatgtt cagtttaatc tctttttcca gaccaaactg tttcaggaag ccatagaccg
     7741 tcgcggcatc gtaatcatat tgatgtttgg tcggttcttg cggtttcggt tcgataagca
     7801 acgtgccctg gaaaccgatt ttatgtttat gctcaaccac catctgcata aagcggccca
     7861 gttgttcacg ctcctgacgc aagtcggtat ttaacagcgt ttcgtaacct tcacgaccgc
     7921 cccacaggac atagttttca ccgcccaatt tatgggttgc ttccatcgct gtaacaactt
     7981 gcgttgccgc ccagctgaag acttcaggat ctgggttcgt cgccgcaccc gcgccgtagc
     8041 gagggtttgt aaagcagttg gcggttcccc acagcagctt cacgccgctc tcttcttgct
     8101 tgcctgccag gacatcaacc atttgcgcaa aattattgat gtactctttt aacgacgcgc
     8161 cctcagggga aacatccaca tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa
     8221 actcaaatgc gacatctgct ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc
     8281 acggacgatt aaacgccccc acaccaaaca tatccgcccc gttccagcag aaggtgtgcc
     8341 agtagcaggc ggcaaaacgc aagtgctctt ccatacgctt acccaacacc agttcgtcgg
     8401 gattgtagtg acggaatgct aacgggtttg aggattttga gccttcataa cgaacgcgat
     8461 cgagctggtc aaaataggct tgcatattga actccataat caggtaatgc cgcgggtgat
     8521 ggatgatgtc gtaatattgg gcactccctt tcagttgctc aattatgtta tttcacactg
     8581 ctattgagat aattcacaag tgtgcgctcg ctcgcaaaat aaaatggaat gatgaaactg
     8641 ggtaattcct cgaagagaaa aatgcaataa gtacaattgc gcaacaaaag taagatctcg
     8701 gtcataaatc aagaaataaa ccaaaaatcg taatcgaaag ataaaaatct gtaattgttt
     8761 tcccctgttt agttgctaaa aattggttac gtttatcgcg gtgattgtta cttattaaaa
     8821 ctgtcctcta actacagaag gccctacacc atgaaaataa agaacattct actcaccctt
     8881 tgcacctcac tcctgcttac caacgttgct gcacacgcca aagaagtcaa aataggtatg
     8941 gcgattgatg atctccgtct tgaacgctgg caaaaagatc gagatatctt tgtgaaaaag
     9001 gcagaatctc tcggcgcgaa agtatttgta cagtctgcaa atggcaatga agaaacacaa
     9061 atgtcgcaga ttgaaaacat gataaaccgg ggtgtcgatg ttcttgtcat tattccgtat
     9121 aacggtcagg tattaagtaa cgttgtaaaa gaagccaaac aagaaggcat taaagtatta
     9181 gcttacgacc gtatgattaa cgatgcggat atcgattttt atatttcttt cgataacgaa
     9241 aaagtcggtg aactgcaggc aaaagccctg gtcgatattg ttccgcaagg taattacttc
     9301 ctgatgggcg gctcgccggt agataacaac gccaagctgt tccgcgccgg acaaatgaaa
     9361 gtgttaaaac cttacgttga ttccggaaaa attaaagtcg ttggtgacca atgggttgat
     9421 ggctggttac cggaaaacgc attgaaaatt atggaaaacg cgctaaccgc caataataac
     9481 aaaattgatg ctgtagttgc ctcaaacgat gccaccgcag gtggggcaat tcaggcatta
     9541 agcgcgcaag gtttatcagg gaaagtagca atctccggcc aggatgcgga tctcgcaggt
     9601 attaaacgta ttgctgccgg tacgcaaact atgacggtgt ataaacctat tacgttgttg
     9661 gcaaatactg ccgcagaaat tgccgttgaa ttgggcaatg gtcaggagcc aaaagcagat
     9721 acctcactga ataatggcct gaaagatgtc ccctcccgcc tcctgacacc gatcgatgtg
     9781 aataaaaaca acatcaaaga tacggtaatt aaagacggat tccacaaaga gagcgagctg
     9841 taagcgttac gccccggcgc ggagcggggg cgtgatttct ctccatgccg cgtgaatgaa
     9901 ttggcttagg tggagtcgtt atgccttatc tacttgaaat gaagaacatt accaaaacct
     9961 tcggcagtgt gaaggcgatt gataacgtct gcttgcggtt gaatgctggc gaaatcgtct
    10021 cactttgtgg ggaaaatggg tctggtaaat caacgctgat gaaagtgctg tgtggtattt
    10081 atccccatgg ctcctacgaa ggcgaaatta tttttgcggg agaagagatt caggcgagtc
    10141 acatccgcga taccgaacgc aaaggtatcg ccatcattca tcaggaattg gccctggtga
    10201 aagaattgac cgtgctggaa aatatcttcc tgggtaacga aataacccac aatggcatta
    10261 tggattatga cctgatgacg ctacgctgtc agaagctgct cgcacaggtc agtttatcca
    10321 tttcacctga tacccgcgtt ggcgatttag ggcttgggca acaacaactg gttgaaattg
    10381 ccaaggcact taataaacag gtgcgcttgt taattctcga tgaaccgaca gcctcattaa
    10441 ctgagcagga aacgtcggtt ttactggata ttattcacga tctacaacag cacggtatcg
    10501 cctgtattta tatttcgcac aaactcaacg aagtcaaagc gatttccgat acgatttgcg
    10561 ttattcgcga cggacagcac attggtacgc gtgatgctgc cggaatgagt gaagacgata
    10621 ttatcaccat gatggtcggg cgagagttaa ccgcgcttta ccctaatgaa ccacatacca
    10681 ccggagatga aatattacgt attgaacatc tgacggcatg gcatccggtc aatcgtcata
    10741 ttaaacgagt taatgatgtc tcgttttccc tgaaacgtgg cgaaatactg ggtattgccg
    10801 gactcgttgg tgccggacgt accgagacca ttcagtgcct gtttggcgtg tggcccggac
    10861 aatgggaagg aaaaatttat attgatggca aacaggtaga tattcgtaac tgtcagcaag
    10921 ccatcgccca ggggattgcg atggtacccg aagacagaaa gcgcgacggc atcgttccgg
    10981 taatggcggt tggtaaaaat attaccctcg ccgcactcaa taaatttacc ggtggcatta
    11041 gccagcttga tgacgcggca gagcaaaaat gtattctgga atcaattcag caactcaaag
    11101 ttaaaacgtc gtcccccgat cttgctattg gacgtttgag cggcggcaat cagcaaaaag
    11161 cgatcctcgc ccgctgtctg ttactcaacc cgcgcattct cattcttgat gaacctacca
    11221 ggggtattga tattggcgcg aaatatgaga tctacaaatt aattaaccaa ctcgtccagc
    11281 agggtattgc cgttattgtt atctcttccg aattacctga agtgctcggc cttagcgatc
    11341 gtgtactggt gatgcatgaa gggaaactaa aagccaacct gataaatcat aacctgactc
    11401 aggagcaggt gatggaagcc gcattgagga gcgaacatca tgtcgaaaag caatccgtct
    11461 gaagtgaaat tggccgtacc gacatccggt ggcttttctg ggctgaaatc actgaatttg
    11521 caggtcttcg tgatgattgc agctatcatc gcaatcatgc tgttctttac ctggaccacc
    11581 gatggtgcct acttaagcgc ccgtaacgtc tccaacctgt tacgccagac cgcgattacc
    11641 ggcatcctcg cggtaggaat ggtgttcgtc ataatttctg ctgaaatcga cctttccgtc
    11701 ggctcaatga tggggctatt aggtggcgtc gcggcgattt gtgacgtctg gttaggctgg
    11761 cctttgccac ttaccatcat tgtgacgttg gttctgggac tgcttctcgg tgcctggaac
    11821 ggatggtggg tcgcgtaccg caaagtccct tcatttattg tcaccctcgc gggcatgttg
    11881 gcatttcgcg gcatactcat tggcatcacc aacggcacga ctgtatcccc caccagcgcc
    11941 gcgatgtcac aaattgggca aagctatctc cccgccagta ccggcttcat cattggcgcg
    12001 cttggcttaa tggcttttgt tggttggcaa tggcgcggaa gaatgcgccg tcaggctttg
    12061 ggtttacagt ctccggcctc taccgcagta gtcggtcgcc aggctttaac cgctatcatc
    12121 gtattaggcg caatctggct gttgaatgat taccgtggcg ttcccactcc tgttctgctg
    12181 ctgacgttgc tgttactcgg cggaatgttt atggcaacgc ggacggcatt tggacgacgc
    12241 atttatgcca tcggcggcaa tctggaagca gcacgtctct ccgggattaa cgttgaacgc
    12301 accaaacttg ccgtgttcgc gattaacgga ttaatggtag ccatcgccgg attaatcctt
    12361 agttctcgac ttggcgctgg ttcaccttct gcgggaaata tcgccgaact ggacgcaatt
    12421 gcagcatgcg tgattggcgg caccagcctg gctggcggtg tgggaagcgt tgccggagca
    12481 gtaatggggg catttatcat ggcttcactg gataacggca tgagtatgat ggatgtaccg
    12541 accttctggc agtatatcgt taaaggtgcg attctgttgc tggcagtatg gatggactcc
    12601 gcaaccaaac gccgttcttg attttgatta aaattttctc aaagccggtt acgtattacc
    12661 ggttttgagt ttttgcatga ttcagcagga aaagaaccat gtttactaaa cgtcaccgca
    12721 tcacattact gttcaatgcc aataaagcct atgaccggca ggtagtagaa ggcgtagggg
    12781 aatatttaca ggcgtcacaa tcggaatggg atattttcat tgaagaagat ttccgcgccc
    12841 gcattgataa aatcaaggac tggttaggag atggcgtcat tgccgacttc gacgacaaac
    12901 agatcgagca agcgctggct gatgtcgacg tccccattgt tggggttggc ggctcgtatc
    12961 accttgcaga aagttaccca cccgttcatt acattgccac cgataactat gcgctggttg
    13021 aaagcgcatt tttgcattta aaagagaaag gcgttaaccg ctttgctttt tatggtcttc
    13081 cggaatcaag cggcaaacgt tgggccactg agcgcgaata tgcatttcgt cagcttgtcg
    13141 ccgaagaaaa gtatcgcgga gtggtttatc aggggttaga aaccgcgcca gagaactggc
    13201 aacacgcgca aaatcggctg gcagactggc tacaaacgct accaccgcaa accgggatta
    13261 ttgccgttac tgacgcccga gcgcggcata ttctgcaagt atgtgaacat ctacatattc
    13321 ccgtaccgga aaaattatgc gtgattggca tcgataacga agaactgacc cgctatctgt
    13381 cgcgtgtcgc cctttcttcg gtcgctcagg gcgcgcggca aatgggctat caggcggcaa
    13441 aactgttgca tcgattatta gataaagaag aaatgccgct acagcgaatt ttggtcccac
    13501 cagttcgcgt cattgaacgg cgctcaacag attatcgctc gctgaccgat cccgccgtta
    13561 ttcaggccat gcattacatt cgtaatcacg cctgtaaagg gattaaagtg gatcaggtac
    13621 tggatgcggt cgggatctcg cgctccaatc ttgagaagcg ttttaaagaa gaggtgggtg
    13681 aaaccatcca tgccatgatt catgccgaga agctggagaa agcgcgcagt ctgctgattt
    13741 caaccacctt gtcgatcaat gagatatcgc aaatgtgcgg ttatccatcg ctgcaatatt
    13801 tctactctgt ttttaaaaaa gcatatgaca caacgccaaa agagtatcgc gatgtaaata
    13861 gcgaggtcat gttgtagcga tgatgagaat tgtcggcgtc acatcaggga actcagcaca
    13921 gccacctgat gcgagcaaaa gccttatccg accgacaaat cagataaggc tcaaggaaat
    13981 gcaattacat atgcgccgcg attaaccgtt ggttatcctg gtacattgcg aacaggtagt
    14041 tattataact cttccctttg gtcgagtagc ccttcagctt gtgaatcatc gctgtggcag
    14101 tcacttcctg atccgcttta cgcagctgcg cacgcgattt acggaacgaa gagtaagccg
    14161 gatgcgtatt gaggttagtg acataggcgc tcaccgattc tttgacagaa ctaaactgtg
    14221 agtacccttt cactttaccc ggcgcattgg tacaacgtcc tttcatgcat ttcatgccga
    14281 aaagattgtt gttgttgcgc gccagcttcg acgttcccca accgctttct gctgcagcca
    14341 tcgtcgccac catactggta gggataatgt ctacgcgttc aagcaaggta ttccacggga
    14401 tttttcgcgt attaccagac cacttcacct tgtagcgttt ggcgatgtct ttcagacgcg
    14461 cacgctcagc aggtgaccat tggccctgat actgttttga aatcagccag ttacgttctg
    14521 cagtaatggc tgcattttgg ctggtaatgt aaggcattac ggtccggaga aacgcctttt
    14581 tccttggtgt tccggaaggg tattttcgca aatcaggaag tgaactactc tttgcactat
    14641 tgcgagaata ctcttgttta ctgcttacct gtttattact agctttagtt aagtgggact
    14701 tttgactcgc tgttgttgtg tgcgtcttcg ctaacacctc actcgaaaac accagagtga
    14761 gtaacataag aatcatcgcc ccatatcgtc gtatgggagt caaaatcatc aggtctcctg
    14821 gtcggattta atcattccaa caccttatat ttttcacaaa tttgagagtt gaatctcaaa
    14881 tcatatcaaa aatagctgtc aagagcaccc caaggaatag tccaaatctg aaactatgtc
    14941 acgtgttaac gattcagatt ggcgctaaat cgcagaaaat gtggggttta tcgcaaaatt
    15001 cagccgtttt ttgcgcgaga tcgctcaccc ttgcttctca tcctgtggac ttaccgctca
    15061 gggatgagtt ttgtctggct tatcgctggc aaactgtctg aaatcgcagc aataaggact
    15121 catccaccat gaaactcgcc gcctgttttc tgacactcct tcctggcttc gccgttgccg
    15181 ccagctggac ttctccgggg ttccctgcct ttagcgaaca gggaacggga acatttgtca
    15241 gccacgcgca gttgcccaaa ggtacgcgtc cactcacgct aaattttgac caacagtgct
    15301 ggcagcctgc agatgcgata aaactcaatc agatgctttc cctgcaacct tgtagcaaca
    15361 cgccgcctca atggcgattg ttcagagacg gcaaatatac gctgcaaata gacacccgct
    15421 ccggtacgcc aacattgatg atttccatcc agaacgccgc cgaaccggta gcaaacctgg
    15481 tccgtgaatg cccgaaatgg gatggattac cgctcacgct ggatgtcagc gccactttcc
    15541 cggaaggagc cgccgtacgg gattattaca gccagcaaat tgcgatagtg aagaacggtc
    15601 aaataacgtt acaacccgct gctaccagca acggtttact cctgctggaa cgggcagaaa
    15661 ctgacgcccc tgcccctttc gactggcata acgccacggt ttactttgtg ctgacagatc
    15721 gtttcgaaaa cggcgatccc agtaatgacc agagttacga acgtcataaa gacggtatgg
    15781 cggaaattgg cacttttcac ggcggcgatt tacgcggcct gatcaacaaa ctggattacc
    15841 tccagcagtt gggcgttaat gctttatgga taagcgcccc atttgagcaa attcacggct
    15901 gggtcggcgg cggtacaaaa ggcgatttcc cgcattatgc ctaccacggt tattacacac
    15961 aggactggac gaatcttgat gccaatatgg gcaacgaagc cgatctacgg acgctggttg
    16021 atagcgcaca tcagcgcggt attcgtattc tctttgatgt cgtgatgaac cacaccggct
    16081 atgccacgct ggcggatatg caggagtatc agtttggcgc gttatatctt tctggtgacg
    16141 aagtgaaaaa aacgctgggt gaacgctgga gcgactggaa acctgccgcc gggcaaacct
    16201 ggcatagctt taacgattac attaatttca gcgacaaaac aggctgggat aaatggtggg
    16261 gaaaaaactg gatccgtacc gatatcggcg attacgacaa tcctggattt gacgatctca
    16321 ctatgtcgct ggcctttttg ccggatatca aaaccgaatc aacgaccgct tctggtctgc
    16381 cggtgttcta taaaaacaaa acggataccc acgctaaagt catcgacggc tttacccctc
    16441 gcgattactt aacccactgg ttaagtcagt gggtccgcga ctatgggatt gatggttttc
    16501 gggtcgatac cgccaaacat gttgagttgc ccgcctggca gcaactgaaa accgaagcca
    16561 gcgccgcgct tcgcgaatgg aaaaaagcta accccgacaa agcattagat gacaaacctt
    16621 tctggatgac cggtgaagcc tggggccacg gcgtgatgca aagtgactac tatcgccacg
    16681 gcttcgatgc gatgatcaat ttcgattatc aggagcaggc ggcgaaagct gtcgattgta
    16741 ttgcgcagat ggatacgacc tggcagcaaa tggcggagaa attgcagggt ttcaacgtgt
    16801 tgagctacct ctcgtcgcat gatacccgtc tgttccgtga agggggcgac aaagcagcag
    16861 agttattact attagcgcca ggcgcggtac aaatctttta tggcgatgaa tcctcgcgtc
    16921 cgttcggtcc tacaggttct gatccgctgc aaggtacacg ttcggatatg aactggcagg
    16981 atgttagcgg taaatctgcc gccagcgtcg cacactggca gaaaatcagc cagttccgcg
    17041 cccgccatcc cgcaattggc gcgggcaaac aaacgacact ttcgctgaag cagggctacg
    17101 gctttgttcg tgagcatggc gacgataaag tgctggtcat ctgggctggg caacagtgac
    17161 ttttccggct tcccgttcgt cagtacctcg ggaagccgcc aaccaggata aaatgtcagc
    17221 cctaatcagc gttgcaggat aaagcaccac tcactcttca acagaccgat ttgcacccca
    17281 gcaaatgtag cgttattgtt accttccttg ctacagagtt cgacagatat cccgctatga
    17341 cattctccct ttttggtgac aaatttaccc gccactccgg cattacgctg ctgatggaag
    17401 atctgaacga cggtttacgc acgcctggcg cgattatgct cggcggcggt aatccggcgc
    17461 agatcccgga aatgcaggac tacttccaga cgctactgac cgacatgctg gaaagtggca
    17521 aagcgactga tgcactgtgc aattacgacg gtccacaggg gaaaacggag ctactcacac
    17581 tgcttgccgg aatgctgcgc gagaagttgg gttgggatat cgaagcacag aatattgcac
    17641 taacaaacgg cagccagagc gcgtttttct acttatttaa tctatttgcc ggacgccgtg
    17701 ccgatggtcg ggtcaaaaaa gtgctgttcc cgctggcacc ggaatacatt ggctatgctg
    17761 acgccggact ggaagaagat ctgtttgtct ctgcgcgtcc gaatattgaa ctgctgccgg
    17821 aaggccagtt taaataccac gtcgattttg agcatctgca tattggcgaa gaaaccggga
    17881 tgatttgcgt ctcccggccg acgaatccaa caggcaatgt gattactgac gaagagttac
    17941 tgaagcttga cgcgctggcg aatcaacacg gcattccgct ggtgattgat aacgcttatg
    18001 gcgtcccgtt cccgggtatc atcttcagcg aagcgcgccc gctgtggaat ccgaatatcg
    18061 tactgtgcat gagtctttcc aagctgggtc taccaggctc ccgctgcggc attatcatcg
    18121 ccaatgaaaa aatcatcacc gccatcacca atatgaacgg cattatcagc ctggcacctg
    18181 gtggtattgg tccggcgatg atgtgtgaaa tgattaagcg taacgatctg ctgcgcctgt
    18241 ctgaaacagt catcaaaccg ttttactacc agcgtgttca ggaaactatc gccatcattc
    18301 gccgctattt accggaaaat cgctgcctga ttcataaacc ggaaggagcc attttcctct
    18361 ggctatggtt taaggatttg cccattacga ccgagcagct ctatcagcgc ctgaaagcac
    18421 gcggcgtgct gatggtgccg gggcacaact tcttcccagg gctggataaa ccgtggccgc
    18481 atacgcatca atgtatgcgc atgaactacg tgccggagcc ggagaaaatt gaggcggggg
    18541 tgaagattct ggcggaagag atagaaagag cctgggctga aagtcactaa aaattgcctg
    18601 atgcgctacg cttatcaggc ctacatgatt tctgcaatat attgaatttg gaagaatttg
    18661 taggccggat aaggcgttta cgccgcatct ggcataaaca aagcgcacta ttccggcgtt
    18721 gagaaacgcc ggaatacgtt tcaaacaggc tgctgccgta gccgatgccg ctgcactttc
    18781 tcgacatcca tgcactgcaa cgcctttgtc gggcaggctt caacacaggc cggtcccgtc
    18841 gcccgatgcc agcacagatc gcacttaatc gccctcgcct ttttgcgcga agaaacgacc
    18901 tccatcgcac caaacgggca agcaagcata cagcttttac agccaatgca acgtgattgt
    18961 tcaacgaaaa tatgcccatg ttcgcggctt atcgcgtcaa cagggcagac attggcgcac
    19021 ggtgcatctt cacactgatg acaggctact gccgtggtcc agctgtggtc tttaatgaca
    19081 cgaatacggg aaataaactc gtctggtgac aacgctgcgc aatcctgatt ctcctgatgc
    19141 gacactgcgc aagccacttc acaggtacgg caaccgatac atttcgtcgc atccgcaata
    19201 ataaaccggt tcatgatcac tcctttgcac taaaacagca gagtgccaga gagcgcgtcc
    19261 ctactgcttt gatccaacga aggaaatgtt aaagttttgg cacttgtcca aagcgttatg
    19321 tgatcgcgcc caggtcatcc ctgacagtaa atcccagttc attagaaata gcctgcgcgg
    19381 tttcacgcag tggtttcagg agatttttct cccccacctg tttcagacgt gatgtcgaaa
    19441 gcgaaatcga cacggcgtac ggcacccgcc catgaatatc aaacaccgga acagcaatac
    19501 aggagacgcc gagttcgttt tcttctctgt ccatcgccgc tccgctttca cgaatgtgcg
    19561 ccagttcgtc gaacatcgcg ggcagctcgg taatggtatt gcgggttaac ggctggatct
    19621 catgctgatg gctttcccag tatgacttca cgtagtccgg gtgaccaaac gccatgtaga
    19681 tcttgcccat tgcggaacag tagagcggca tatgctggcc aatataggca cgggttcgca
    19741 gcatcccggt tgtgggttcc agcttataaa tcaaaatagc gtgatcgtct tcgcggctgg
    19801 agaagttaat ggtttcacca gtggcgatgt tcagtgcctc aagatgcgga gcggcgatat
    19861 gaatgatatt cagcgaagac agcgccttct gcccgacggc aataaatttg gtagtcaggc
    19921 ggtaactccc tgcggcgggc gcggtggtca catagccgca ggactgtaat ccctgcaata
    19981 agcgatggac ggtactctta tttaaaccag ccagctccga aagatgcgcc aacggacaac
    20041 cgtttggata gttgctcaaa atctcaatca gcatcaaccc gcgaaacaga ctctggcttc
    20101 cggctggacg ctctttttcc tgcgccatct cgttctcttt ttttcccatc acttctttcc
    20161 ccattttgtc gcgtcctgat ggtagcgcaa agtgtgccgt agttcacgat ctcgacagat
    20221 aatttataac c
//
LOCUS       DAOFQD010000083        20214 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.083, whole genome shotgun sequence.
ACCESSION   DAOFQD010000083 DAOFQD010000000
VERSION     DAOFQD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.083"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..701
                     /locus_tag="QBF46_003115"
     CDS             <1..701
                     /locus_tag="QBF46_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDQ6675265.1"
                     /translation="QNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYLEIMAT
                     KYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAII
                     IVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERPIWVIS
                     CLMIVLWSGIILHGRNKLLELAGKCHNEELWSPVRCAFTGALTLYYYFIGFFGILAAI
                     AITLVWQRHTRFFH"
     gene            complement(737..1693)
                     /locus_tag="QBF46_003116"
     CDS             complement(737..1693)
                     /locus_tag="QBF46_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDQ6675266.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1693..2934)
                     /gene="clsB"
                     /locus_tag="QBF46_003117"
     CDS             complement(1693..2934)
                     /gene="clsB"
                     /locus_tag="QBF46_003117"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDQ6675267.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHARHFNQTLRD
                     NLNGIIAVDCKQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2931..3692)
                     /locus_tag="QBF46_003118"
     CDS             complement(2931..3692)
                     /locus_tag="QBF46_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDQ6675268.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3825..4235
                     /locus_tag="QBF46_003119"
     CDS             3825..4235
                     /locus_tag="QBF46_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDQ6675269.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4197..5303)
                     /locus_tag="QBF46_003120"
     CDS             complement(4197..5303)
                     /locus_tag="QBF46_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6675270.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5314..6447)
                     /locus_tag="QBF46_003121"
     CDS             complement(5314..6447)
                     /locus_tag="QBF46_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6675271.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6440..8176)
                     /locus_tag="QBF46_003122"
     CDS             complement(6440..8176)
                     /locus_tag="QBF46_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6675272.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8169..9164)
                     /gene="hlyD"
                     /locus_tag="QBF46_003123"
     CDS             complement(8169..9164)
                     /gene="hlyD"
                     /locus_tag="QBF46_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDQ6675273.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(9073..9837)
                     /gene="cecR"
                     /locus_tag="QBF46_003124"
     CDS             complement(9073..9837)
                     /gene="cecR"
                     /locus_tag="QBF46_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CecR"
                     /protein_id="HDQ6675274.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLIFKDY
                     RKGVCSDEKTCRDRIGGSGTCRRGCRRLLVVSKPPG"
     gene            10066..11430
                     /gene="rhlE"
                     /locus_tag="QBF46_003125"
     CDS             10066..11430
                     /gene="rhlE"
                     /locus_tag="QBF46_003125"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDQ6675275.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11662..12144)
                     /gene="ybiA"
                     /locus_tag="QBF46_003126"
     CDS             complement(11662..12144)
                     /gene="ybiA"
                     /locus_tag="QBF46_003126"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HDQ6675276.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12264..14414
                     /gene="dinG"
                     /locus_tag="QBF46_003127"
     CDS             12264..14414
                     /gene="dinG"
                     /locus_tag="QBF46_003127"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDQ6675277.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14442..15404
                     /gene="ybiB"
                     /locus_tag="QBF46_003128"
     CDS             14442..15404
                     /gene="ybiB"
                     /locus_tag="QBF46_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDQ6675278.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15545..16630
                     /gene="hcxB"
                     /locus_tag="QBF46_003129"
     CDS             15545..16630
                     /gene="hcxB"
                     /locus_tag="QBF46_003129"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDQ6675279.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(16859..17119)
                     /locus_tag="QBF46_003130"
     CDS             complement(16859..17119)
                     /locus_tag="QBF46_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ6675280.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17384..17650)
                     /gene="ybiI"
                     /locus_tag="QBF46_003131"
     CDS             complement(17384..17650)
                     /gene="ybiI"
                     /locus_tag="QBF46_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDQ6675281.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17724..18401)
                     /gene="ybiX"
                     /locus_tag="QBF46_003132"
     CDS             complement(17724..18401)
                     /gene="ybiX"
                     /locus_tag="QBF46_003132"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDQ6675282.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18443..>20214)
                     /locus_tag="QBF46_003133"
     CDS             complement(18443..>20214)
                     /locus_tag="QBF46_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDQ6675283.1"
                     /translation="MISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNV
                     MGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPG
                     YSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWS
                     RVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGS
                     IGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFA
                     IYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVD
                     TAKSGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKP
                     QKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVA
                     GNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGA
                     GARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASI
                     NKSGYRYHPGEPRTFLLTANMHF"
BASE COUNT         4697 a         5316 c         5452 g         4749 t
ORIGIN      
        1 tgcaaaacag caataaatta gattttcagc acgagaccag gatattaaac ggtatatggc
       61 tcattaccgc tttaggtttg gtggcaaccg caggactggc ctggggagct aagtatctcg
      121 aaattatggc aaccaaatat gattcaccac caatgtatgt cgccataggg ttattattac
      181 tttgtatgta tggcttaatt aaggatatca acaagataaa tgccgccatc gcaggcgtaa
      241 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
      301 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
      361 tattatttaa tattgatcct ggttctcacc gttttatgat tatgatgacg ttgacagggt
      421 tggccctggt aatcatcgtg aatgccgcat taatgagtga acggcccatt tgggtaataa
      481 gttgcttaat gattgtgtta tggtcaggca ttatcttgca tggacgaaat aagctccttg
      541 aattggcggg gaaatgccat aatgaagagt tgtggagtcc ggttcgttgc gcttttacag
      601 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgca gcgatagcta
      661 taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg cacatactaa
      721 agaacttaac taaacttcac atcgccgctt catttttcgc ccgcagcttc ttcgcctggc
      781 tttccagtaa caggtaacag atcagcgcca gcagcagtgg gataaagtaa tacaggacgc
      841 gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca gccagtagtg
      901 cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg acgccagcaa
      961 tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga ccaagtaaca
     1021 gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc tgagcgaggg
     1081 cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg cggtgcttcg
     1141 cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg ccgagaatac
     1201 gcagcgtagt ttgatcgaca taccagtgat ccggcaactc caccacgcct gcggtaaaga
     1261 taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc gagaaaatcc
     1321 gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc ataccaatgc
     1381 cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac accagcatca
     1441 cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca tagcagccgt
     1501 aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg cgattgtagt
     1561 cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc accagcaaca
     1621 caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc caacgcgggt
     1681 gtgatttact catcagggtt ttacccccgt gttttcagtt tctacccgat cctgcgtttc
     1741 cattgtcggt tgtgcggggg gatcaacctg cgccagacgt ggtgtgtgtg ccggaagcca
     1801 gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt tggtcaggtt
     1861 ccaccaggtg cgtttcggca gcatggtctc atcgacctgc ttacaatcta cggcaataat
     1921 gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga gcgtggatga tgacatttgc
     1981 ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg tcgcccagtg
     2041 atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa atacctgaac
     2101 gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa tcggcatatc
     2161 cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt tacgcaaggc
     2221 gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatgactt ctcgccgcgc
     2281 ctgagtgagc attttcagat agtgacgttc aatatcatcg cgatgttctt cgttatcgcg
     2341 ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt tgtgatgacg
     2401 tcgccaccag cgtcgtgccg cgctttgtcc tggcaggttt tccagctcaa actggagaat
     2461 atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct ctggaccgta
     2521 gctggacata tgctcggcgg agtaattcag cccgccaata aaggctatac gcgcgtcgat
     2581 caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa aaaggcgagg
     2641 gcggggatcg tagtagcgaa ataccacgcc agctgccgtc agttcattga caaactcatc
     2701 gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc cgcgttgcgc
     2761 tgctgccagt agtgccgcat gtagttgttt gccgacgtca tcctcaaacc agataaacgt
     2821 ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg gataatattg
     2881 ctcgccgttt tccagcaact ggatcttatt gccttctcgc cagctacatt tcataaatga
     2941 atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag cggcaacgcg
     3001 gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag tagaggaaat
     3061 tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc atccagtccg
     3121 gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc acccgccacc
     3181 aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc aagctgcgcc
     3241 tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc ttttccggtc
     3301 atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc gaccgaaaca
     3361 tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc gtgatgccct
     3421 tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc ggcgagaaac
     3481 tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc gtgcgcgccc
     3541 atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcaccgcgtc gcgaagttcc
     3601 ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat ggtgagcacc
     3661 ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc tcttatctca
     3721 ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt ccgtaaagtt
     3781 cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag tggcaacaac
     3841 gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta ctggtagtgg
     3901 cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg ggattatgcg
     3961 cactgtattg tgtcacggta gtgatgatgt tggtgtttca gcgccacccc gagcaacgct
     4021 ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt ggcgcagcgc
     4081 tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg gcaattgcag
     4141 ggctggcaat ccttgccggc ccggcggtag tgtctttgct ggcgaaagat aagaagttac
     4201 atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt tatcaccagt
     4261 agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata aatctgctta
     4321 gtaatgtccg taaagtggcg aatagggtta atccacgtca ggttttgcag ccatactggc
     4381 atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat aaaaacaaac
     4441 acgccgataa acgcctgctg ttgtgttgaa cagagtgatg aaatcaacag accgaatccc
     4501 accagcgata aaccgtaaat caccatcgta aagtagaaca gtgccagcga tccggcgaag
     4561 gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa ggtggcgaca
     4621 attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg cgaaaccagt
     4681 agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt gacgatcatt
     4741 acgccgatag tggtgatcat ggcgatcagt gacggcacca caaaccattt gtagtcgaga
     4801 ttcgggttat accagttgcg taccaccagc tcgctgttgt taggtttcgg ttttccttcc
     4861 agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc aatttgcgca
     4921 ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa ggtatccagt
     4981 ttgcgcgaga agtcagccgg gaaacgcacc aggagtaacg ccttttgtgt gtcgatggtt
     5041 gggcggatct cctgtgggct tttcagcagc agcacatgag taaaggcgct ggcgcgggca
     5101 aaacgttggg tcagctccac cgaatgctcg ccgttatctt catcgtagat ggcgatggtg
     5161 gcgttagtca cttccagcgt ggcggcgaac gggaacagaa tcacctgaat aagcacaggt
     5221 aaaatcagaa tcgcgcgggt ttgcggttcg cgcagcagcg actgcaactc tttgcggatt
     5281 aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt tggttttcag
     5341 ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt ttaccaccag
     5401 caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga aataacgagc
     5461 gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct gaaaaataaa
     5521 gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag cggcattgaa
     5581 ctggttgcgg gtaatcgtgg aaatcagcag ccccatcccc agggtactga gtaaaaacag
     5641 gctggagata aaaaacagaa tcagcagcga cccgcgatac ggcacgccga gaataaacac
     5701 tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag ggatcagctt
     5761 acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca tggtgccgcg
     5821 ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg tcatgataat
     5881 ggtcaccgca ccggggataa tgaagtgctg gctaatcgcc gccgggttaa accagtagcg
     5941 ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc gctgcatttg
     6001 ccagatctgc cagatccctt cgacataccc ctgtacaaag ttagcggtat tcggctcact
     6061 gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct gttccgcaaa
     6121 atccaccgga ataaccacca gaccgcgaat tttccccgcc tgcattttgg cgatcagttc
     6181 ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg tgtgggtgaa
     6241 atccagcgcc gcttcgctac gctgttccag taaaatcccg acccgcagct tgctggagtc
     6301 gaggttaatg ccgtaaccaa aaataaacag cagtagcagc gggatcacta ccgcaatcag
     6361 ccagctactc ggatcgcgaa cgatctgccg cgtctcttta acgcacagcg cccgtacgcg
     6421 acgccaggac aggatcgggt tactcattgc tatgctcctt atcccagtcg tggatcaact
     6481 gaataaaggc ttgctccatg gtgggatcgg gttgctcgtc gttggccgac tgcgctttca
     6541 aatcatccgg cgtgccgctg gcaattaatt tcccacggta caccaggccg atgcggtcgc
     6601 aatattctgc ttcatccata aagtgagtgg tgaccatcac cgtgacgcct ttctctacca
     6661 tgctgttgat atgcagccaa aattcgcggc gggtgagggg atcaacgccg gacgttggtt
     6721 cgtcgagaaa cagaatgtcc ggttcatgca tcagcgaaca ggccagcgcc agccgctgtt
     6781 taaaacctaa tggcagttca tcggtcgcgt gggaggcgat acttttcagg ccaaatgcct
     6841 cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac acaccagaga
     6901 aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt tgcgccatat
     6961 agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc agcaccagcg
     7021 cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc gatttacccg
     7081 cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag ttgacgtgat
     7141 cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc accgtctcgc
     7201 cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag gttccggcac
     7261 cacccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg atgttgattt
     7321 ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg aggatcagac
     7381 gtaccgattt cccctgaatc atgccgtcgc tgacctgcgg cagtttcaag gcgcgttgca
     7441 acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg gccatggttt
     7501 gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc agtaacacgt
     7561 cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac atcccttcgc
     7621 ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca acgccgacgc
     7681 cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc aggccgagtt
     7741 tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc ccaagagacg
     7801 taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg ctgcgcaaat
     7861 ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca aatttctgcg
     7921 gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca aagccaatca
     7981 ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc agcgtggttt
     8041 tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg gtacaatcga
     8101 gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg ttcagcgtga
     8161 taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg cattccctgg
     8221 cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag gtcggtacgc
     8281 agatccggcg tttcgacggt tttcggggta aattcagcag tcggcgaaac gaaaccaatc
     8341 tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt gcgccccggc
     8401 tgggcctggt caagattacg ttcatcaaca taagcgcgca cccacaccgg acgcgttagt
     8461 gaaacggtaa acaccgtgcc accttcattg aggaccgtgc ctggctccac cgcgcgcgtt
     8521 aacagcgtgc catcagacgg ggctatcaac gttgagtcct gtaaattcaa ctccgcctgc
     8581 gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc ctgttcacgg
     8641 ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc ctgcgcctgg
     8701 tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tagtgcggct tttccacaac
     8761 ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc ctgtttcacc
     8821 gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag gtcatactgc
     8881 gcctgtgcca ccgaaacacc cgctttcgcc tgcatcaggg caatctcata cggcttgtga
     8941 tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac cgccagcgat
     9001 tcaacgcgcc ccccaacacg gaaactaaga tttaccgtac gaatatccac gttgccatac
     9061 agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc aaccacggcg
     9121 gcaagtacca ctaccgccaa tccgatcacg acaggttttt tcatcactac aaactccttt
     9181 gcgataatcc ttgaaaatca ggtcgatatg acaggtcacc gtctggttga tcagttcggt
     9241 cttttcttca tcgaacgcgg tccagccggt acgtaacaga attgtttctt tgccaagacg
     9301 gaacgccaga atctcaccaa tcaacgcatg ggtatgaagg atcatgcggg tgtcattggc
     9361 gtcgcagccg gtccaggcgg caatcaggcg tgtcaggtgg ctgtgtagcg gactaatcac
     9421 ctgttcgtgc accaggtggt aggctgccgt gggagagagc tgctcacggg agataaactt
     9481 gctgaggttg acggtgtcat cctgggtgag cagcttaatc atgttcctgc aggcgcgaag
     9541 gatcagttca cggatggcag cccgatcagg ctgtggttgc gcgaacaagc gttcggcttc
     9601 ctcggcatgc ggacggaact gctcgccaat aaaatcggca atccactggg cgcaggcgag
     9661 gtacaaatct tctttcgaac cgaagtagta ggtgatggca gcgatattct gcccggcctg
     9721 ggcggctatc tcgcgagtgg tggcgttcat tccatattca ccaaactgcg ccagtgcggc
     9781 agcaatcagc tgttttttcg cctgttcacc cttgattgtc atggcaggat tattcatcgc
     9841 acagtccatt cttaatcaaa tgattgatta agattatgac tccatagggg agttgtccag
     9901 tatggctaag aattttagca acgccagtca cagggataat ttatgcgctg cgtcacaaaa
     9961 actgctacac tccgctccct catgacattg tggtttttgt cattttcctt ttcagtatct
    10021 ccctgaaaac tacaccggta acggtcgggg cggttcggag tagttatgtc tttcgattct
    10081 ttgggtttaa gccctgatat cctgcgcgcc gttgccgagc agggttaccg tgaacccacc
    10141 cctattcagc agcaggcgat ccctgcggtg ctggaaggcc gcgacctgat ggctagcgcc
    10201 cagaccggca ccggcaaaac agcgggcttt acgctgccgc tgttgcaaca cctgatcact
    10261 cgccagccgc acgccaaagg gcgtcgtccg gtacgtgcgc tcattcttac cccgacccgt
    10321 gaactggcgg cgcagattgg cgaaaacgtc cgtgattaca gcaaatacct gaacattcgt
    10381 tcgctggtgg tgtttggtgg tgtgagtatt aacccgcaga tgatgaaact gcgtggcggc
    10441 gttgatgtgc tggtggcaac gccgggacgt ttgctggacc tggaacatca gaatgcagtg
    10501 aagctggatc aggttgaaat cctcgtcctc gatgaagctg accgcatgct cgacatgggc
    10561 tttatccacg atatccgtcg cgtgttaaca aaactacctg cgaagcgcca gaacctgtta
    10621 ttctccgcga ccttctctga cgatattaaa gccctggcgg aaaaactgtt gcacaacccg
    10681 ctggaaatcg aagtggcacg ccgcaatacc gcgtctgatc aggtgaccca gcacgttcac
    10741 tttgtcgata agaaacgcaa acgcgaattg ctgtcgcaca tgattgggaa agggaactgg
    10801 cagcaggtgc tggtgtttac ccgtaccaaa cacggcgcta accatctggc tgaacagctc
    10861 aataaagatg gcatccgtag tgcggcgatc cacggcaata aatcgcaagg tgcgcgtact
    10921 cgtgcgctgg ctgattttaa atcgggcgat attcgtgtac tggtggcaac tgacatcgct
    10981 gcgcgcggcc tggatattga agagctgccg cacgtcgtca actatgaact gccaaacgta
    11041 cctgaagatt atgtccaccg tatcgggcgt accggtcgtg cggctgctac cggtgaagcg
    11101 ttgtcgctgg tgtgtgttga tgaacacaaa ctgctgcgtg atatcgaaaa actgctgaaa
    11161 aaagagatcc cgcgcattgc gattccgggc tatgagccgg acccgtcaat caaagccgaa
    11221 ccgatccaga acggtcgcca gcaacgtggc ggcggcggtc gtgggcaagg tggtggtcgc
    11281 ggtcaacagc aaccacgccg tggggaaggt ggcgcaaaat ctgcaagcgc gaaacctgca
    11341 gaaaaaccgt ctcgccgcct cggcgatgcc aaaccggcag gcgaacaaca acgtcgccgc
    11401 cgtccgcgta aacctgccgc tgcgcagtaa tcttttatgc cgggctatgc ccggcatcag
    11461 gctgatgaac aaacgcaaaa ctgcctgatg cgctacgctt atcaggccta cgtgaactct
    11521 gcaatatatt gaatttgcat gcttttgtag gccggataag gcgtttacgc cgcatccggc
    11581 atttcacaac aagcacttgt cagcaatttg agaacacggg aaaaaattta tctgttttac
    11641 cgcccataag ccaccgagac gttacttctc tatagccaat tgttcgcgca actccattaa
    11701 aaggtagccc agtcgattct tgcccttacc attaccaccg tctccccagt aagcatcgtt
    11761 ttccgtatgc tcaaccagtt ttgcgggcgc ggttgccagc aagagcgcac gcagttctgc
    11821 atgctgttcg aatttagcgc gaagtgcttt tcgcatcacc tgttctttga ccgactccca
    11881 gtttttacgc agaggcttag aacgatcgcg ccccatgcgt gcggcgacca taggggaaga
    11941 aacccgacgg atctcttcgc ggtatttttc atcaaggaat ttttgtgcct gaaaatagtg
    12001 ttctgaggtg ggccaggttt tcccgtcaac cttgatgggc caggcggcaa agttagaaaa
    12061 atcaccgtag tcatcgctgg tgctgtagaa atttatgatg gtgtcttgca tgacgtgttg
    12121 gatcctttgt gctcgaacgg gcattaaacc gcattatgtt ggtggttatt gcgagccgct
    12181 ttccagaaac agaaaaacca ttacccctga aaaccgaaaa atgccacaat attggctgtt
    12241 tatacagtat ttcaggtttt ctcatggcat taaccgccgc gcttaaagcg caaattgccg
    12301 cctggtataa ggcgcttcag gaacagatcc ccgactttat tccccgtgcg ccgcagcggc
    12361 agatgattgc ggacgtcgcc aaaacgctgg ccggagaaga agggcggcat ctggcgattg
    12421 aagcccccac cggcgttggg aaaacgctct cctatttgat ccccggcatc gccattgccc
    12481 gcgaagagca aaaaacgctg gtggtgagta ccgccaacgt ggcgttgcag gatcagattt
    12541 acagcaaaga tttaccgctg ctgaaaaaga tcattcccga tcttaaattc actgccgctt
    12601 tcgggcgtgg gcgctacgtt tgtccgcgta atctgacggc gctcgccagc actgaaccca
    12661 cgcaacagga tctgctggcg tttcttgacg acgaactgac gccaaataat caggaagagc
    12721 aaaaacgttg tgcgaagctg aagggcgatc tcgacactta taaatgggat ggtctgcgcg
    12781 atcatacgga tatcgctata gatgatgatc tctggcgtcg tttgagtacc gacaaagcca
    12841 gctgcctcaa ccgtaactgt tactactatc gtgaatgccc gttttttgtc gctcgtcggg
    12901 agattcagga agcggaagtg gtggtggcaa accatgcgct ggtgatggcg gcgatggaaa
    12961 gcgaagcggt attgcctgac ccgaaaaatt tactgctggt gctggacgaa ggccatcacc
    13021 tgccggatgt ggcgcgggat gcgcttgaga tgagcgccga aatcaccgcg ccgtggtatc
    13081 ggctacagct ggacttgttc acgaaactgg tcgctacctg catggagcag tttcgcccga
    13141 agaccatccc accgctggcg atccctgaac gtttgaatgc gcattgtgaa gagttgtatg
    13201 agcttatcgc ctcgttaaac aacattctca atctctacat gcctgccggg caggaggcag
    13261 agcaccgttt tgcgatgggc gaactgccag atgaagtgct ggagatctgc cagcggctgg
    13321 caaaactcac cgagatgctg cgtggcctgg cggagttatt tcttaacgat ttaagtgaga
    13381 aaaccggcag ccatgacatt gtacgtctgc atcggttgat tttgcagatg aaccgcgcgt
    13441 tggggatgtt cgaggcgcaa agcaaactct ggcggctggc ttcgctggcg caatcttccg
    13501 gtgcaccggt gaccaaatgg gcgacgcggg aagagcgcga agggcagcta cacctctggt
    13561 ttcactgcgt gggaatacgt gtcagcgatc agctggaaag gctgctgtgg cgcagtattc
    13621 cgcacattat tgtcacctcc gcaaccttgc gttcgctgaa cagtttttcg cgtttgcagg
    13681 agatgagtgg tctgaaagag aaagcgggcg accgttttgt ggcgctggat tcccccttta
    13741 accactgcga acagggcaaa attgttattc cccggatgcg cgttgagcct tccatcgaca
    13801 acgaagagca gcatattgcc gaaatggcgg cctttttccg taagcaggtg gagagcaaaa
    13861 aacatctcgg tatgttggta ctgtttgcca gcggacgggc gatgcagcgc tttctcgact
    13921 atgtgacgga tttacgtctg atgttgctgg ttcagggcga tcagccgcgt taccgtttag
    13981 ttgaactgca ccgcaaacgc gtcgccaacg gtgagcgcag cgtgctggtg ggcttacagt
    14041 catttgccga agggcttgat ttgaaaggtg atctgctcag ccaggtgcat atccacaaaa
    14101 tcgcttttcc gcccatcgac agcccggtgg tgatcaccga aggggaatgg ctgaaaagcc
    14161 tcaaccgcta tccgtttgag gtgcaaagcc tgccgagcgc ctcgtttaac ctgattcagc
    14221 aggttgggcg actgattcga agccacggtt gctggggcga agtggttatc tacgacaaac
    14281 gcttgctgac caaaaattat ggcaagcgac tactggatgc attaccggta tttccgatag
    14341 agcaaccgga agtccctgaa ggtatagtta aaaagaaaga aaaaacgaaa tccccacgcc
    14401 gtcggcggcg ttaatgatgt gagtcaggta aggagtcgta aatggactat cgcaaaatca
    14461 ttaaagagat cgggcgcggg aaaaaccacg cgcgcgattt agaccgggat actgcgcgcg
    14521 gtctgtatgc tcatatgctc aatggtgaag tccctgacct tgagttgggc ggcgtattga
    14581 ttgcgctgcg tatcaaagga gaaggggaag cagagatgct cggcttttac gaagccatgc
    14641 aaaatcacac catcaagctg acgccgccag caggcaagcc gatgccgatt gtcatcccca
    14701 gttacaacgg cgcgcgtaaa caggccaacc tgacgccgtt gttggcgatt ctcctgcaca
    14761 aactcggttt tcctgtggtg gttcacgggg ttagcgaaga tccaacccgc gtgctgactg
    14821 aaaccatttt tgaattgatg ggtattacgc caacgttgca cggcgggcag gcgcaggcga
    14881 agctcgacga acatcaaccg gtgtttatgc cagtcggcgc gttttgcccg ccgctggaaa
    14941 aacaactggc gatgcgctgg cggatgggcg tgcgtaacag tgcacatacc ctggcgaaac
    15001 tggcgacgcc atttgccgaa ggtgaggcgc tgcgtctttc cagcgtttcg catccggaat
    15061 acattggacg cgtcgcgaag ttctttagcg atatcggcgg gcgggcgcta ctaatgcatg
    15121 gtactgaagg tgaagtgtat gctaatccgc agcgctgccc gcagatcaat ctcattgacc
    15181 gtgaagggat gcgggtgctg tatgaaaaac aggacactgc tggtagcgag ttactgccac
    15241 aagcaaaaga tccggaaacc acggcgcagt ggattgagcg ttgccttgct ggcagcgaac
    15301 cgattcccga atcgctgaaa atccagatgg cttgctgcct ggtggctacg ggtgaagcgg
    15361 caactatcag cgacggcctg gcgcgcgtta atcaggcatt ttaattcttt tctcagccgg
    15421 atgagccatg tcatccggct tttaccccac cgcaatatga aattcctgca tctttattga
    15481 ccttcccacg cccggcgtgc agcataaaaa tacaacaaac acataacata aacaggagtt
    15541 aaccatggaa agtggtcatc gctttgatgc tcagacgctg cacagtttta ttcaggctgt
    15601 atttcgtcag atgggtagcg aggaacaaga agcgaaatta gttgccgatc atttaatcgc
    15661 ggcaaacctg gcagggcatg attcacatgg tattggcatg atcccaagct atgtacgctc
    15721 ctggagtcag gggcacctgc aaattaacca tcatgccaaa accgttaaag aggcgggggc
    15781 ggcggtcacg ctcgatggcg atcgcgcatt tggtcaggtc gcggcacatg aagcgatggc
    15841 gctggggatt gagaaagcgc atcagcacgg tattgccgcc gtggcgctac ataactcgca
    15901 tcatatcggc cgtatcggtt actgggcgga gcagtgtgca gcggcggggt ttgtctctat
    15961 ccactttgtt agcgtggtcg gtattccaat ggtcgcgccg ttccacggtc gcgacagccg
    16021 ctttggcacc aatccgttct gtgtggtttt ccctcgtaaa gataatttcc cgctgttgct
    16081 tgattacgcc accagcgcca ttgcatttgg caaaacccgc gtcgcctggc ataaaggcgt
    16141 ccccgtgccg ccaggttgcc tgattgacgt taacggcgtg ccgacgacca atccggcggt
    16201 aatgcaggag tcgccgttgg gttcgctgtt gacctttgcc gaacataaag gctacgccct
    16261 tgcagcgatg tgtgaaattc ttggcggggc gctttccggc ggtaaaacga cgcatcagga
    16321 aacgttacaa accagtcccg atgccattct taactgcatg accactatca tcatcaaccc
    16381 ggaactcttc ggcgcgccgg attgtaacgc gcagaccgaa gcctttgccg agtgggtgaa
    16441 agcctcgccg catgatgatg ataagccgat tttgctaccg ggcgagtggg aagtgaacac
    16501 gcgtcgcgaa cggcagaagc aggggattcc actggatgcg ggaagctggc aggccatttg
    16561 tgatgcagcg cggcagattg gtatgccgga agagacgttg caggctttct gtcagcagtt
    16621 agccagctaa aaaaaagccc gtccagtggc ggacgggcaa acaagggtaa cataggatca
    16681 atgagggtta gagcatatgc gtctgtcggc aaacagacag ggaaatactt gtgctggacg
    16741 tagcgtaaac gcctgatccg tccaacggtt cggattttgt aggcctgata agacgcggta
    16801 gcgtcgcatc aggcatctgt tgccggatgc ggcgtaaacg ccttatccgt cctacgaatt
    16861 acttataaat taccgcagta ccgcttaatt tattgttgtt ggtggcggaa gtgatgctgt
    16921 atccactggc accggctgcc gcggctttct cagccagttt cgcttccagg gcatcgaggg
    16981 tggatgcgcc atcggcagaa accacgccga ttttattcat gttctgtgcc tgggatgccg
    17041 ttaccggttc cgccgcgaac acgccaaatg acagagttga aagagccata gcagcaacaa
    17101 cagtattgat agttttcata attaatctct cgcaggtgat ttttgtataa gtgacgttgt
    17161 ttcgtcgatg tgatgagtat cacgtttttt tacgagagat aaaatcgaag agaattgacc
    17221 gccttgttca aataaattga ttgatatcta acctactgat aaaccattta tttgttgtac
    17281 ttatgaactt gttttatacc gcgtggcagt cacagcagcg ttaaaggcac ggaatgccac
    17341 gcaaaataca aattatactc gctatgcatc tcgccagttg tagttaacgt aactggctgt
    17401 ctttcgaacc tctgcgatta tatcctgtat aagctggttt ttgtaaatct ttctcctgct
    17461 gacaatgaat acataagcgc acgccaggaa tggcttcccg acgggcctgc gggatggggg
    17521 caccgcactc ttcacattca tccaggcttt cgccgcgcgg aatttcaccc cgagcgcggg
    17581 caatcgcatc ttcaattgta ctgttgatct gttcgttgac ggcgtcatcg ttagcccaac
    17641 cggatgccat atcgacctcc ccatatcaat acttgtacag ttaagtgtag ctaatccagg
    17701 gacgaactcg ggcagttcaa gcatcagatc tccgaccatt cccgcagcag attatgataa
    17761 agattaagca gcgacaggat ctcttcactt tcgccgtagc ggcttttcag cgactgaata
    17821 ttgttgtcca gttcaaacag catggcgcgc tttttatcat cgcggatcat cgactggatc
    17881 cacataaatg atgccactcg tacgccgcgg gttacgggtg tcacgcaatg caggctgctg
    17941 gagggataca acacgagatc gcctgccggg agttttaccc gatgttgtcc gaaggtgtca
    18001 ttaacgacca gttcgccgcc gtcgtagctt tgtggatcgc ttaaaaacag cgtggcagaa
    18061 aggtcagtac gcatccagcc gttttgtgga tggctgcgta ctgcgccatc cacatggaaa
    18121 ccataggttt cattgttctg atagcgatta aacagcggcg tggaaagggt acgcggcaag
    18181 gccgcggcaa agaataaagc atgttggtta accgcgttca gcacctcatt ttgcagggcg
    18241 gcgtataacg tgctgcgggt gtcgacctgt tgattgttct taacttgcgc accttgtgcg
    18301 ccggtggtga cgcgtccatc cacccattcg gcttgttcca gttgttcgcg aaaacgagcg
    18361 acgtcctgtg gcgataacac gccgggaatg tggtacatca tacaatttct ccaaaaagtg
    18421 gggcctgcgc cccacatctg aatcagaaat gcatattggc tgtgagcaag aaggttcttg
    18481 gctcgcccgg gtgataacgg tagccgctct tgttgattga ggcgacgtaa tcggtatcaa
    18541 acaggttgta aacgtttagc tggaagtcga gattgcgatt aactcgatac cccagtttgg
    18601 catcggcgac ccagtaacct tcggtaaacg ctggcgttcc caccgcgccg tctgaacctt
    18661 tatgcatact gccgatatag cgtgcgcccg cgccaacaga gatatcgtcg gttgcctgat
    18721 attggctcca taaggtgaag gcgtgctccg gggtatacgg cagcgatgag gaaccatcct
    18781 gggcaacatc tttgccgttt ttgatggttg ctttttgctg ggtatagccg ccaatcacct
    18841 gccacgcggg agtgatattc ccggccacgg atatctcata gccttcgacg cgtttcttac
    18901 cgtattgcga gtaagttccg tcatcatttt gctcaacttc attttcgata tcagtacgga
    18961 acagcgcggc ggtgagcaac agacgtttat ccagaacctg ccatttggtg ccaatctcgc
    19021 tggtgttggc tttttgcggt ttaaaatcgg tgcggttggc actgttaccg ctgccagact
    19081 gcgcaagggc gaagttgttg ccgcccggag gctgctggga aacggcatag ttaatataga
    19141 cattgccgtt ttccgtcagg tgatacagcg ccccggcttt ccagttcatc agattgcccg
    19201 acttggcggt gtcgacggtg gtgaccggag aaccttttgc cacaccagtt gggcaggtga
    19261 tggcaccgcg tccgctgccg ccgcaggcgg tggcactgtc atattcagta tgataattat
    19321 ccagacggat cccgccgttc agctcaaaat cacgggtgat ttgcagcgta tcaaaggcgt
    19381 aaattgcgaa ggtatccgtc tgaccattgg cgtttgcgcc gttgcgcgtc aggccgccgg
    19441 gatgaatgct gctgtcagga tgataaatat ttaccgcggg taacgtcacc ggattaacgc
    19501 cgtagttagt ctgcgtttca cgggtaaatt ccacgccggt actgacatca tgaccgatag
    19561 aaccggtata gaacgtcgag gtcaggttgg tctggttggt gagaatttta ttactcacat
    19621 ctttggtatt cgccgtgcgt gaccaggtcc agctattcac atcgctggtg ggctgagtaa
    19681 tattcgacgc cccgcccata atcgccgtca tcaggtaatc ctgctttacg cgcgaccaac
    19741 gggtagtatt gcgaatggtg gtgttatcgt tgatgtcgtg ctcaaaacgc atggtggcgg
    19801 tgtcggtggt cgaatcgtcg taatcggaat ccgtgccgta aaagttatga gtatcaactt
    19861 ttccggaatg attcagggcc gccgttcccg cagatggggc agaatagccc ggcaaaccga
    19921 tggtcggaat gccgccgtct ggcgtgttgt gctgggtgac atgcagataa ttaagataca
    19981 aacgattcgc tgtaccaagg ccaaaagcga cagaaggggc gacgccgtaa cgctcatttt
    20041 tgactttgtc gcgtccggca tcgtgcgttt tttcgcccat tacattcagg cgcaccgcgg
    20101 tggtatcacc aatgacctga ttgacgtcca gcgtgccgcg gcggaaccag gcgctgccaa
    20161 tactggcgga ggcgtcaatg ccggaatcat tgcgcggctg cttgctgatc atat
//
LOCUS       DAOFQD010000084        20184 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.084, whole genome shotgun sequence.
ACCESSION   DAOFQD010000084 DAOFQD010000000
VERSION     DAOFQD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.084"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            84..389
                     /locus_tag="QBF46_003134"
     CDS             84..389
                     /locus_tag="QBF46_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDQ6675284.1"
                     /translation="MHEKENLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            376..852
                     /locus_tag="QBF46_003135"
     CDS             376..852
                     /locus_tag="QBF46_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDQ6675285.1"
                     /translation="MQNLNPQRKAFLDMVAWSEGTDNGRQPTRNHGYDIIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEVEL"
     gene            836..1297
                     /locus_tag="QBF46_003136"
     CDS             836..1297
                     /locus_tag="QBF46_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDQ6675286.1"
                     /translation="MKLSYKLVIAAFFVTVIGSFICSANYYHSKAIEYKKQRDENAMA
                     LDSAMATISDMQKRQRDVAELDARYTKELADANATIESLRADVSSGRKRLQVAATCAK
                     STTRASGMGDGESPGLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLR"
     gene            1565..1789
                     /locus_tag="QBF46_003137"
     CDS             1565..1789
                     /locus_tag="QBF46_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675287.1"
                     /translation="MLENYFPSGIGAQPVTQAQKQRVIAVQAALELVKASLSSAGGQA
                     TSAKFEQELKAAIGLIEPLADAIQKAISKE"
     gene            1956..2306
                     /locus_tag="QBF46_003138"
     CDS             1956..2306
                     /locus_tag="QBF46_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ6675288.1"
                     /translation="MPPRTPKACRFRGCRNTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDSIRAGVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADCNLQSLC
                     WPCHKAKTARERLK"
     gene            2432..2926
                     /locus_tag="QBF46_003139"
     CDS             2432..2926
                     /locus_tag="QBF46_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDQ6675289.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGKKKATNPFLK
                     IIES"
     gene            3160..4656
                     /locus_tag="QBF46_003140"
     CDS             3160..4656
                     /locus_tag="QBF46_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDQ6675290.1"
                     /translation="MPITLEPWQLFVICCAFGWVNKGTRLRRFREVYTEIPRKNGKSA
                     ISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPARLMCKRTPMLTEAFGIEVNAS
                     NMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHATDALYTTMLTGMGARRQPLMWA
                     ITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIYTVDEGDDWTDPQVLEKANPNI
                     GVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSARSAYFNLVSWQSCEDKSLTLE
                     QFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYYSVAPRFWVPYDTVYSVEKNED
                     RRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAANKISPVSESPIDPFGATGLSHD
                     LADEDLNPVTIVQNFANMSDPMKELEAAIESGRFHHDGNPIMTWCIGNVVGKTIPGND
                     DLVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLSDHIESYGIRSL"
     gene            4668..4850
                     /locus_tag="QBF46_003141"
     CDS             4668..4850
                     /locus_tag="QBF46_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675291.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            4850..6091
                     /locus_tag="QBF46_003142"
     CDS             4850..6091
                     /locus_tag="QBF46_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ6675292.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRAAGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLS
                     QKDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            6069..6719
                     /locus_tag="QBF46_003143"
     CDS             6069..6719
                     /locus_tag="QBF46_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDQ6675293.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            6734..7939
                     /locus_tag="QBF46_003144"
     CDS             6734..7939
                     /locus_tag="QBF46_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDQ6675294.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVEAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLTTEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITIGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTSGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDNEGNYLWRPGLELGQPSSLLGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAEK"
     gene            7989..8216
                     /locus_tag="QBF46_003145"
     CDS             7989..8216
                     /locus_tag="QBF46_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675295.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKAAKEKKPRPVKRDENASDTGRD"
     gene            8191..8514
                     /locus_tag="QBF46_003146"
     CDS             8191..8514
                     /locus_tag="QBF46_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDQ6675296.1"
                     /translation="MLLTLEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDESIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVSPY
                     RYFPQ"
     gene            8511..8921
                     /locus_tag="QBF46_003147"
     CDS             8511..8921
                     /locus_tag="QBF46_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDQ6675297.1"
                     /translation="MKISQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VAFSTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITSDYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGSNGDFLFAR"
     gene            8896..9402
                     /locus_tag="QBF46_003148"
     CDS             8896..9402
                     /locus_tag="QBF46_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675298.1"
                     /translation="MATSFLHVDFQQPTEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMMETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            9399..9959
                     /locus_tag="QBF46_003149"
     CDS             9399..9959
                     /locus_tag="QBF46_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675299.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQALKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLDPGNGPDGDIEHHTEITIPS"
     gene            9968..10138
                     /locus_tag="QBF46_003150"
     CDS             9968..10138
                     /locus_tag="QBF46_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDQ6675300.1"
                     /translation="MFVKPVKGRSVPDPARSDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            10122..11618
                     /locus_tag="QBF46_003151"
     CDS             10122..11618
                     /locus_tag="QBF46_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDQ6675301.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHASNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATV
                     TLTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIKDAINAVPALPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            11618..11974
                     /locus_tag="QBF46_003152"
     CDS             11618..11974
                     /locus_tag="QBF46_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDQ6675302.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            11974..12243
                     /locus_tag="QBF46_003153"
     CDS             11974..12243
                     /locus_tag="QBF46_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDQ6675303.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQNSVDQMSPADLNAAAWLVAGFFLQA"
     gene            12385..14217
                     /locus_tag="QBF46_003154"
     CDS             12385..14217
                     /locus_tag="QBF46_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDQ6675304.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKVMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDVKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETISPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPPRGYTPYPEVDPAQANNASGATGPNPFMIN
                     KATAPKVDGEIKVSFINMPPGMRVTETRSSGIDINHDVGYTRFW"
     gene            14309..14839
                     /locus_tag="QBF46_003155"
     CDS             14309..14839
                     /locus_tag="QBF46_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675305.1"
                     /translation="MKGYITASVILGAAAIFSSLIISGNISFKDEHIIQLSGGAIKLG
                     DVYKENKLISAKIIFPDNQGEQILVVDGNPENFKEDFQEKLNKVIKTLNASKKKDEEK
                     VSLDNLSVIEESKLELVSAVRYSAQYVPMFTLTLDKKEITMPKNTVIFPFASDETAKY
                     LNEQQQKYKDSLFLTR"
     gene            14901..16229
                     /locus_tag="QBF46_003156"
     CDS             14901..16229
                     /locus_tag="QBF46_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HDQ6675306.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIEALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVIGNASIMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNMVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVGQSTTPAQSSGWPAVTHPVLNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            16226..17305
                     /locus_tag="QBF46_003157"
     CDS             16226..17305
                     /locus_tag="QBF46_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDQ6675307.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSDAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGFGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKTRVQEDPF"
     gene            17305..17853
                     /locus_tag="QBF46_003158"
     CDS             17305..17853
                     /locus_tag="QBF46_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HDQ6675308.1"
                     /translation="MKAIETIQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTRAGIVVDGAGKTITFRNAPKARFEMDLEVTGQVKDLCDSSGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            17853..18278
                     /locus_tag="QBF46_003159"
     CDS             17853..18278
                     /locus_tag="QBF46_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDQ6675309.1"
                     /translation="MELWLTVNGKRTCASAPMDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            18265..19323
                     /locus_tag="QBF46_003160"
     CDS             18265..19323
                     /locus_tag="QBF46_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDQ6675310.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTTTDDATSSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIEPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPTD
                     NISIAKNELAVLGTISWT"
     gene            19314..19898
                     /locus_tag="QBF46_003161"
     CDS             19314..19898
                     /locus_tag="QBF46_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDQ6675311.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDANITRYDKSTFTCSSVCTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            19902..>20184
                     /locus_tag="QBF46_003162"
     CDS             19902..>20184
                     /locus_tag="QBF46_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675312.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTAL"
BASE COUNT         4881 a         4935 c         5902 g         4466 t
ORIGIN      
        1 aatgtaattt tcgaattaat aataaatcaa catatcattt cacgctccga ttttctgtcg
       61 gaggcgaaac tgcggctatt catatgcacg aaaaagagaa tcttgctgga gcgttctggc
      121 tcgttttgct gatcatcgca ggttggggcg gtctggtccg ctacctgata gatgtgaagc
      181 agagtaaagc aacgtggagt tggataaatg ctctggctca gatagtggta tcaggattca
      241 ccggtgttat tggtggcctg atcagcatcg aaagtggatt cagtatttac atgattctcg
      301 cgacagcggg gattagtggt gcgatgggtt cggttgcact gacgtacttc tgggaacgac
      361 tgacaggggt gaaaaatgca aaatcttaat cctcagcgta aagctttcct cgatatggtg
      421 gcatggtcag aaggaacgga taacgggcga caaccgacac gtaaccacgg ttatgatatt
      481 attgtcggtg gtgaactctt cactgattac tccgatcacc ctcgcaaact tgtcacgcta
      541 aaccccaaac tcaaatcaac agctgcaggc cgttatcaac ttctttcacg ctggtgggat
      601 gcttaccgca agcagcttgg gctgaaagac ttctctccaa aaagccagga tgctgtggca
      661 ttgcagcaga ttaaagagcg tggcgcttta ccgatgattg atcgcggcga tattcgtcag
      721 gctatcgatc gttgcagcaa tatctgggct tcactgccgg gcgctggtta cggtcagtat
      781 gaacataaaa tcggtgacct gattgcccga ttcaaagagg ctggcggggt ggtaaatgaa
      841 gttgagctat aagctggtta tcgctgcatt cttcgttact gtcattggtt cttttatctg
      901 ttcagcaaat tattaccaca gcaaagccat cgaatacaaa aagcagcgtg atgagaatgc
      961 tatggcatta gattcggcta tggcgactat ctctgatatg cagaagcgtc aacgtgacgt
     1021 agcagaactt gacgccagat acacaaagga gcttgctgat gctaacgcga ctatcgaaag
     1081 tctccgtgct gatgtttctt ctgggcgtaa gcgcctgcaa gtcgccgcca cctgtgcaaa
     1141 atcaacgacc agagccagcg gcatgggcga tggagaaagc ccaggactta cagcagatgc
     1201 tgaactcaat tattaccgtc tccgaagtgg aatcgacagg ataaccgcac aggttaacta
     1261 cctgcaggag tacatcagga cgcaatgcct tcgatgatag cgataatttt actcatcatc
     1321 cttcacatca ggccctgtag acagagtggt gattacttct ggagtggatt cagattaaac
     1381 atctcattgc taatgtttga ggttaagcat ctggcgcgcg gtaagtggtt gcgttgaaat
     1441 aagagccagt ccattacaaa tttcatcaag cgttattggt aagggtgata attatgagat
     1501 acctcttcac taagagtaat cttaagggta atgtgtaacc tcacttttta atacggaggt
     1561 tgtaatgtta gagaattatt tcccttcagg aataggtgct cagcctgtaa ctcaagctca
     1621 aaaacagagg gttatagctg ttcaagctgc ccttgaatta gttaaggcct cactttcttc
     1681 tgcaggtggt caggctacaa gtgcaaaatt tgaacaggaa ttaaaagcag ctattggctt
     1741 aattgaacca ttagccgatg ccattcagaa agcaatcagc aaagaataaa taaaccacct
     1801 gcattttgtt ttatttccca actaacgggt gtaataatct taatgtttcc cgtggataga
     1861 taagacaata agtattcacc ttcaagatat aaacgagcct cgctaatgcg aggctttttt
     1921 acgcattgta agcgtgcacc aaagagggga tataaatgcc accacgaacc ccaaaagcct
     1981 gccgttttcg cggttgccgc aataccacga ctgatccgtc aggctattgc gaaagccaca
     2041 aaagcgaggg ctggaagcaa tacaagccag gacaatcccg tcatcagcgc ggttatggtt
     2101 cgaagtggga cagtatccgc gcgggtgtcc tgaagcgtga caaaggcctg tgtcagttat
     2161 gtctgcgtgc tggtgtggtg cgtgaggcga aaaccgttga ccacatcatc cctaaagcgc
     2221 atggcggcac tgatgccgac tgtaatctgc agagtctgtg ctggccgtgt cataaggcga
     2281 agacggcccg tgaacgactt aagtgataat aattctcaac tgtctgaggg gaggggcggg
     2341 tcaaatctct gtgacctgac gtcttccgga ctgcccgccc catcgttttt ttatacccgc
     2401 gaaaaatgaa atttaaccag gagtgccgca tatggctgga acggcggggc gttccgggcg
     2461 tcgccccaag ccaacggcgc gcaaggcgct ggccggaaac cccggcaagc gagccctgaa
     2521 taaagatgaa cctgttttta cgcccatcaa aggtgttgag ccaccggagt ggttcgctga
     2581 agaagatctc cctctcgcta cgatcatgtg gcaactgaca actaaagaac tctgcggtca
     2641 gggcctgctg tgcgtgactg accttgcggt gcttgagcgg tggtgcgtgg cctacgagtt
     2701 ctggcgacgt gccgtgaaaa atattgccag acagggcaac accatcaccg gtgcaatggg
     2761 cggtatggtc aaaaatccgg agctgactgc caaaaaagaa caggagtccg agatgagcag
     2821 tacgggggca atgctcggac tcgaccccag cagccgccag cgtctgattg gcctggcggg
     2881 gaagaagaaa gccactaacc cgtttctgaa aatcatcgaa tcatgagccg gaaatcttac
     2941 cccaacgtaa atgctgcaaa tcagtatgcc cgggatgtcg tgcgcggaaa gattgttgcc
     3001 tgccagtttg tgattcaggc ctgccagcgc catcttgatg acctgatggc ggaaaaaagt
     3061 aagtcgtttc gttaccgctt cgacaaggac ctggctgaac gggccgcgaa atttattcag
     3121 ctgttgccgc acaccaaggg ggagtgggca ttcaaacgga tgcccatcac gctggagccg
     3181 tggcagctat ttgtgatctg ctgtgcgttt ggctgggtca ataaaggcac ccggttgcgc
     3241 cgcttccggg aggtgtacac cgaaatcccc cgtaagaacg gcaaatcagc aatctctgcc
     3301 ggtgttgccc tgtattgttt tgcctgtgat aacgagtttg gcgcggaagt gtattccggt
     3361 gccacgacag agaaacaggc gtgggaagtc tttcgcccgg cgcgactgat gtgtaaacgc
     3421 acacccatgc tgacggaagc gttcgggatt gaggttaacg cctcaaacat gaaccgtccg
     3481 gaggatggcg cgcggtttga accgctgatc ggcaacccag gtgatggttc atcaccccac
     3541 tgtgccgtgg ttgatgaata tcacgagcat gccaccgatg cgctttatac cacaatgctt
     3601 accgggatgg gggcgcgacg tcagccactg atgtgggcca tcaccaccgc cgggtacaac
     3661 attgaggggc cgtgctacga caagcggcgg gaagtcatcg agatgctcaa cggctcggtg
     3721 cctaacgatg aactgttcgg gatcatctat accgttgatg aaggtgacga ctggaccgac
     3781 ccgcaggtgc tggaaaaagc caatccaaat attggcgtgt cggtttatcg cgaatttttg
     3841 ttaagtcagc agcagcgtgc gaaaaataac gcccgtctgg caaacgtctt taaaacaaaa
     3901 cacctcaata tctgggtgtc ggcgcgttcg gcgtatttca acctggtgag ctggcagagc
     3961 tgcgaggata aatcactgac tcttgagcag ttcgaggggc agccgtgcat tctggccttt
     4021 gacctggcgc gtaagctgga tatgaacagc atggcgcgac tttatacccg cgagattgac
     4081 ggtaaaacgc attactacag tgtggccccg cgtttctggg taccgtatga cacggtgtac
     4141 agcgtcgaga aaaatgaaga tcgccggaca gccgaacgct ttcagaaatg ggtggaaatg
     4201 ggcgttctga ccgttaccga tggtgcagag gtggattatc gctacatcct cgaagaggcc
     4261 aaagcggcga acaaaatcag cccggtcagt gagtcaccca tcgacccttt cggagcgacc
     4321 gggctgtcac atgaccttgc tgatgaagac ctgaatcccg ttactatcgt ccagaacttc
     4381 gccaatatgt ccgacccgat gaaagagctg gaggcagcga ttgaatcggg acgctttcat
     4441 catgacggca atcccatcat gacctggtgt atcggcaatg tggtcggcaa aaccattccg
     4501 ggtaacgatg atttagtgaa gcccgtcaaa gagcaggcgg aaaacaaaat cgatggtgca
     4561 gttgcgctga ttatggcggt tggcagagcc atgctgtacg agaaagaaga cacgctgtct
     4621 gaccacattg agtcctatgg gatccgctcg ctttaactga ggtaattatg atcatgctga
     4681 ttctcgcgcc tctggtgggc gtgctggggg cgcttttgct ggcgtatggt gcctggctga
     4741 tttatccccc ggcggggttt gttgttgccg gggcgttgtg tctgttctgg tcgtggctgg
     4801 tggcgcgata tctcgaccgt acacagtcgt ctgtcggcgg aggtaaatag tgttcttttc
     4861 gggattattt caacgaaaaa gtgacgcacc ggtgaccacg ccagcagagc tggcggatgc
     4921 tatcgggttg tcctacgaca cctataccgg aaagcagatc agcagccagc gggccatgcg
     4981 actgacggcg gttttttcct gtgtcagggt gctggcggag tcggtcggga tgttgccctg
     5041 caacctgtat cacctgaacg gcagtctgaa gcagagagcc gctggcgaac gtctgcataa
     5101 gctgatctcc acgcatccca atggctatat gacgccgcag gagttctggg agctggtggt
     5161 cacctgtctg tgcctgcggg gaaactttta cgcctacaaa gtgaaagcat ttggcgaagt
     5221 ggctgaactg ctgcccgtcg atcccggctg tgtggtaccg aagcttaaca gtagctggga
     5281 gccggtctat caggtcacat tcccggatgg ctccacggat gtactgagcc agaaggatat
     5341 ctggcatgtg cgcacgctga cgctggacgg actggtgggg ctgaatccca tcgcctatgc
     5401 ccgcgaggca atatcgctgg cggcagcgac cgaagagcac ggggccagac tgttcagcaa
     5461 tggcgcggtg acgtcgggtg tgttgcgtac agagcagacg ctgtcagatc aggcttatga
     5521 gcgcctgaag aaagattttg aggagcgtca caccgggctt ggcaatgctc accgcccgat
     5581 gatccttgag atggggctgg actggaagtc gatggcgctg aacgccgagg acagccagtt
     5641 cctggaaacc cgcaagtttc agcttgaaga aatctgtcgt ctgttccggg tgccgttgca
     5701 catggtgcag aacaccgatc gcgccacctt caacaatatc gaagagctgg ggctgggatt
     5761 tatcaactat tcactggtgc cgtatctgac ccgcatcgaa cagcggatca acaccggact
     5821 ggtacgaaaa agtaagcagg gcgtttatta cgccaaattt aacgccgggg cgttactgcg
     5881 cggggatatg aagtcccgtt ttgaagccta cgccaccggg atcaactggg gaatttactc
     5941 tcccaatgac tgccgcgacc tggaagatat gaatccacga cccggtggtg atgtctatct
     6001 cacaccgatg aacatgacca cgaaaccctc cgatggcagt aaagccggta agcagaagga
     6061 taacgccaat gcagacgaaa caacgtcttg atgtaccgct gagtctgaaa tctgtcagtg
     6121 actccggtga gtttgaaggg tatggctccg tctttggtgt aaaggacagc cacgatgatg
     6181 tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg gagtgacaga aaagcgttac
     6241 ctgcgctgct ctggcagcac cgcatggatg aacccatcgg tgtttacacc gaaatgaagg
     6301 aagacgatgt cgggctttac gtcaggggac ggttgcttat tgatgatgat cccctcgcaa
     6361 aacgcgcaca tgcacacatg aaggccggtt cgttaaccgg cctttctatt gggtacgtcc
     6421 tgaaagactg ggaatacgac cggagcaaag aagcctttct gctgaaagaa atcgacctct
     6481 gggaagtcag cctggtgacg ttcccgtcta acgacgaggc gcggatcagc gacgtcaaga
     6541 acgcactggc ccgcggggaa atccccgaac agaaaaaaat cgaaagagtc ctgcgtgatg
     6601 tcggactctc ccgtacccag gccaaagcat tcatggccgg gggctatggc gcactgtccc
     6661 tgcgcgacgc tgaggatgtg ggctctgcac tgaatgcact gaaaaatctg aacttctaat
     6721 caggagaaat acgatggcgg ttgatattaa agatgtcgaa caggtcgcgc aggagctgca
     6781 gcagaagttt gacgacttca aagcaaagaa cgacaagcgc gttgaggcga ttgagcagga
     6841 aaaaggcaaa cttgccgggc aggtggaaac cctgaacgga aaactcagcg agctggaaaa
     6901 tctcaaaagc gatcttgaaa aagagctgct tgagctgaaa cgtccggcag gtggtgcgca
     6961 aaataaactg accaccgagc ataaagaagc gtttgtgggc ttcctgcgta aaggccgtga
     7021 agatggtctg cgcgatctgg agcgcaaggc attacaggtg ggcaccgatg aagacggcgg
     7081 ctatgccgtg ccggaagcgc tggatcgcaa cattctgacc ttgctgaaag atgaagtggt
     7141 gatgcgtcag gaagccacgg tgatcaccat tggtggttcc gactacaaaa aactggtgaa
     7201 tctgggcggc acggcttccg gatgggttgg cgagactgac gcgcgctccc agactgccac
     7261 ctcaaaactg ggcctgattg aacctttcat gggggaaatc tacggtaacc cgcaggccac
     7321 ccagaaaatg ctggatgatg cctttttcaa cgtggaagca tggatcaaca gcgagctggc
     7381 aaccgaattt gccgaacagg aagaaattgc ctttacctca ggcgatggca ccaagaagcc
     7441 gaaagggttc ctggcgtatg aatccactga tgaaaccgat aaggtccggg cgttcggcaa
     7501 acttcagcat attgtatccg gcgaagcgac tgcggtgacc gcagacgcca ttatcaaact
     7561 gatttacacg ctgcgtaagg cacaccgcac tggcgcgaag ttcatgatga acaacaacag
     7621 cctgtttgcc attcgtctgt tgaaagacaa cgagggtaac tatctgtggc gtccggggct
     7681 ggaactgggg cagccttctt ctctgctggg gtacggtatt gctgaaaacg aacagatgcc
     7741 ggatattgct gcggatgcga aagccatcgc atttggtaac ttcaaacggg gttacaccat
     7801 cgttgaccgt atcggcaccc gcattctgcg tgacccgtac accaataaac cgtttgtcgg
     7861 tttttatacc accaagcgca ccggcggaat gctggtcgat tcgcaggcca tcaaactgct
     7921 gaagattgca gagaagtaat cgctcatggg cgcggttccg cgccccctgt tctgaagggt
     7981 gaagaatcat gatcctgaaa caagatctta aatggtcacc ggacggtatg cgtgttgagg
     8041 tcattcgggc cggtgagtat gacgacgggg cgcttcctgc ccgggtgcag gagattgcac
     8101 ttcaggccgg gttagcagag cgcggaacca gtgcaaaaag cagtaaagcg gcaaaagaga
     8161 aaaagccacg gccagtaaag agggatgaga atgcttctga cactggaaga gattaaagcc
     8221 caactccggc tggatgagga tttcgatgcc gatgaccgcc atctgcaact gctggcctgt
     8281 gcggcgcaaa agcggacgga aacgtatctg aaccggaagc tctatgcacc ggatgaatcc
     8341 attccggaca gcgatccgga cgggctgcac ctgccggatg atattcgtct tgggatgctg
     8401 atgcttatca gccactttta cgaaaaccgc tcgtcggtta cggaagtgga gaaactcgac
     8461 atgccgcaga gttttggctg gcttgtcagc ccgtacaggt actttccgca atgaaaatta
     8521 gccaggcaca gaccagcgca acctacattc tgccggaccc cggtgaactg aataaacgcg
     8581 tcctgatccg ccagcgggtg gatatgcccg cagataactt tggcgtggag cctcaatacc
     8641 cggttgcgtt cagtacatgg gcgaaggtta tccagaccag tgccaccacc tggcaggaaa
     8701 ccgcgcagac cggagacgcc atcacccatt acatcaccat tcgttaccgc cgggggatca
     8761 cctctgatta tgaggtggtc tgcggtgaca gtgtgtaccg ggtgaaacgt cagcgcgatc
     8821 tgaacggagc gcggcgcttt ctgctgctag agtgtacgga gctgggcgaa tgtaggcaga
     8881 gtcacggagg cagcaatggc gacttccttt ttgcacgttg attttcagca gcccacggag
     8941 atgcgcttta accgcgcccg tgtccggcgg gcgtttgtca cgattggtca gcgtcatatg
     9001 cgtgatgccc gtcggctggt gatgcgccgt gcgcggtcgg caccgggtga aaaccccggt
     9061 tatcagaccg gacgcctggc tcgttcgatt ggttacatgg tacccagagc cagtaaacat
     9121 cgccctggtt ttatggcacg tatagcccct aaccagcgta atggagaggg aaaccgccgt
     9181 atcactggtg atttttatcc ggcttttttg ttctatggcg tgaggcgagg ggcaaagcgt
     9241 cgtcgcagcc atcatcgtgg tgcatccggt ggcagcggct ggcgactggc tccacgtaat
     9301 aacttcatga tggaaacgct tgaaaagaac cgcagctgga cacgctattt tctggcgcgg
     9361 gaattgcgta aatcactgaa gccggagcga cgacacagat gaaactgacg cctgttattg
     9421 ctgcactgcg tgcccgctgt ccgtattttg aaaaccgggt tgcaggtgcg gcccagttca
     9481 aaaatctgcc ggaggtcgga aagctgaaac tcccggcggc atatgttgta ccgggtgatg
     9541 attctccggg agaaaacaaa agccagaccg actactggca ggcgctgaaa gagggtttct
     9601 ccgtggttgt catactgagt aacgggcgtg atgagcgcgg tcagtttgcc tcgtatgatg
     9661 tggtggacga tgtccggcag atgctcttta aggctctgct gggctggaac ccggaggcgt
     9721 gcggtaaccc gattacctat gacggcggca cgctgctgga tctgaatcgt catgagctga
     9781 tttatcagtt cgatttttcg gtcatcagcg agctgactga agacgatacc cgccagcagg
     9841 atgatctgaa cagtctggat gaactgcaaa cgctggcgat tgatgttgat tatctcgatc
     9901 caggtaacgg gcctgacggc gatatcgaac atcacacaga aataaccatt ccttcctgag
     9961 gatcctcatg tttgtgaaac ctgttaaagg gcggtcagtg cctgaccctg cccgcagcga
    10021 ccttttgccc gccgaagggc gaaatgttga cgagaacaac tactggctgc gccgtgaagc
    10081 agcgggtgat atccggcgcg tgaataaaaa ggtgaacacc gatgacgata agctttaaca
    10141 ccattccgtc gaatacgctg gttccgctgt tttatgcgga aatggataac caggcggcga
    10201 atactgcaca ggacagcgga gcatcgctgc tgattggtca tgccagtaac ggtgcagaga
    10261 ttgttgccaa cagtctggtg ctgatgccgt cggcagacta tgcacgccag atttgtggtg
    10321 cgggaagtca gctggcgcgt atggtcgagg cttatcgcca gaccgacccg tttggtgagc
    10381 tgtatgtgat tgccgttccg gaagccacag gcgcggcggc aacggttacg ctgacggtga
    10441 ccggggaagc aaccgaaagc ggcacggtga atgtctatgt gggacgtacc cgcgtgcagg
    10501 ctccggtgac caacggcgat aacgtcacga cgattgccag cagtatcaaa gatgccatca
    10561 atgccgttcc ggccctgccg tttacggctt catcttcggc aggtgtggtc acactgaccg
    10621 cgcgtcataa ggggctttgc gggaatgaaa ttcctgtcag cctcaattac tacggctttg
    10681 gtgggggcga agtgctgcca gcgggcgtac agattgccgt ggcgacgggt accgccggaa
    10741 cgggtgctcc ggttctcacc ggcgcggtgg ctgcaatggc ggatgagccg tttgattata
    10801 tcggcctgcc gttcaacgac acggcctccg ttaacacgct ggtgaccgag atgaacgata
    10861 ccagcggtcg ctggagctat gcgcgtcagc tgtatggtca tgtgtatacg gcaaagatcg
    10921 gcacgctgtc agaactggtg accgcaggtg accagtttaa ccagcagcac attaccctgg
    10981 cggggtacga aaaagagacc cagacgcctg ccgacgaact ggcggcaagc cgtaccgccc
    11041 gcgcagcggt gtttattcgc aacgatccgg cacgtcccac gcagaccggt gagctggtgg
    11101 gtatgctgcc tgcgccgaag gggaaacggt tcacgatgac cgaacaacag accctgctgt
    11161 ctcatggcgt ggcaacggcg tatgtcgaaa gcggggtact gcgcattcag cgtgatgtca
    11221 ccacgtacag gaaaaacgct tacggggttg cggataacag ctacctcgac agtgagacac
    11281 tgcataccag cgcgtatgta ctgcgcaaac tgaaatccgt cattaccagt aagtacgggc
    11341 gtcacaagct tgccagtgac ggtacccgct ttggtcccgg tcaggcgatt gtcaccccgg
    11401 cggtgatcaa aggggaactg ctggcaacct accgtcagct tgagcgtgcg gggatcgtgg
    11461 aaaactacga actgtttaag cagtacctgg ttgtggagcg tgatgccagc gatccgaacc
    11521 gcctgaacac gctgttcccg cctgactatg ttaaccagtt gcgtgtcttt gccgtggtta
    11581 accagttccg tcttcagtat tcagaggagt ctgcataatg gcccgtatcg ggggaacctg
    11641 ttatttcaaa attgacggtc agcagctatc gctgaccggc ggcattgagg tgcccatgaa
    11701 caggacggtc aatgatgaca tcatcggcct ggacggttca gtggaccgca aggaaactca
    11761 ccgtgcgccc tatgtcaaag ggaccttcaa ggtgccgaag aattttccgg tgagcaaaat
    11821 cacctcgtct gatgagatga ccatcactgc cgagctggcg aacggtcagg tctatgtact
    11881 gtcgtctgcc tggctgcacg gcgaagcgaa ccataatgcc gaagaaggca cggttgatct
    11941 tgagttccac ggtgaagaag gggattacca gtaatgaaag agcttgagtt aaagaaaccg
    12001 attactgctc atggcgagac actctccgta cttgagtttg atgagcccac cgggaaagat
    12061 gtccgcgagc tggggtatcc ctaccagatg aatcaggatg agtcagtcag acttctggcg
    12121 catgtggtat cgaaatacat tgtgcggctg gcgaaagtgc cgcaaaactc tgtcgaccag
    12181 atgtctccgg cagacctgaa tgcagcggcg tggcttgtgg ccggtttttt cctccaggcc
    12241 tgacggctga atacctcact gatcgcttct ttgactgcgc cagttactgg cgcattaatc
    12301 ctttcgaatt gctgaatatg ccgatcagtg aaattccctt gctggtcagt caggcaaaca
    12361 ggatagagca ggagaaacgc acacatggct gaatttgagc ttaaggcgtt gatcaccggt
    12421 gtcgacagac tttctcccgc gctgtcgaaa atgcaaaaga aaatccgggg atttaaacgc
    12481 caggcggaag aagcatcaca gggtgggctg gcgcttggtg gcggactggc agcgggtctg
    12541 acgctttccc tgaaatctta tgccgatcag gaaaacgccg ccaccgggct gaaagtcgcc
    12601 atgatggatg cgaacggcga ggtcggaaag agctttcagg acatcaataa actggctatt
    12661 ggcctgggta accagctacc cggtacaacg gctgatttcc agaacatgat gcagatgctg
    12721 gtgcgtcagg ggatcccggc agaaaatatt cttggcggtg tgggtaaagc gacagcttat
    12781 cttgcggtac aactgaaaaa aacaccggaa gcggctgctg agtttgctgc aaagatgcag
    12841 gatgctaccg gaacggcgtc agaagacatg atggggctgt tcgacactat ccagaaggcg
    12901 ttttatctgg gcgttgacga taccaacatg ttgtccttct tcactaaaac cagttctgtt
    12961 ctgaagatgg tgaacaagga cggtcttcag gctgcacaga gccttgcccc catcagcgtc
    13021 atgatggatc agatggggat gaacggggag tcggcaggta atgccctgcg aaaagttatc
    13081 cagtccggat taagcgttaa gaaaatcagg gacgttaata aagttatggc ccgccagaaa
    13141 ctcggggtac agctcgattt tactgacggc aaagggagtt ttggcggtct tgataacatg
    13201 ttcaggcaac tggcaaagct gcgaaaactg accgacgtta agcgaaccgg tgtacttaag
    13261 gcaatatttg gtgatgatgc cgaaaccctt caggtggtca atgctctgat cgataaagga
    13321 aaggatggtt acgatcagat ccagcagaag atgaataaac aggccagcct gaataaacgt
    13381 gttcaggccc agcttggtac gctgtccaac ctgtgggagg caatgacggg gaccgcaact
    13441 aacggccttg cggctattgg cggcgcattt tctggtgacg tcaaaaatat cacgcaatgg
    13501 ctgggggagt taggggaaaa attcacgaag tttgcggatg aaaatccccg ggttattcgc
    13561 ggcgtcgtcg ggcttgctgc cggtcttgcg attctgaaac tgggattgat gggcgtgggc
    13621 agtgccatca gtatcgtcag caggatcatg tcgatgacgc cgattggcat gattgcgacg
    13681 gcgattgccc tggctgcggg attaattatc actaactggg atgttgtcgg accttatttt
    13741 aagaagctct gggaaaccat tagtccttat tttgaggctg gctgggaact tctgaagaag
    13801 gtttttgcct ggtcgccgct ggggatggtg atcaataact ggggaccggt tgttaagtgg
    13861 tttcaggata tgtgggataa gctgaagccg attattgaat ggtttaccga cagttccggt
    13921 gacacggtcg atgccattaa ctcggcgcag tggggcgcgg gtgcttatga tgcttatggg
    13981 acgggaatac cgccacgggg atacactcct tatccggagg tggatccggc tcaggcaaac
    14041 aacgcctccg gtgccacagg tccgaatccc ttcatgatta ataaagctac cgcgccaaaa
    14101 gttgatggtg agatcaaggt atcatttata aatatgccac caggtatgcg ggttacggaa
    14161 acacgctcca gtggcattga tataaatcac gatgttggct atacccgatt ttggtagcca
    14221 ggattcccct cacaggtatt gctggttgta agtcataaat agagtgatag aattaatgca
    14281 catttagaaa aatgttaata ggcgaaaaat gaaaggctat atcacagcaa gtgtaattct
    14341 tggagcagcg gctatttttt catctctcat aatctctggc aacatctcct ttaaagatga
    14401 acatattatt cagttatctg gaggagccat aaaacttggt gatgtttata aagaaaataa
    14461 attgataagt gcaaagatta tttttccaga taatcagggt gaacagattc ttgttgtcga
    14521 cggcaatcct gaaaacttta aggaggattt tcaggagaaa ttaaataaag taataaaaac
    14581 tttaaatgcg tcaaagaaaa aagatgaaga gaaagttagc ctggataatt taagtgttat
    14641 tgaagagtct aaactagagc tcgtttctgc ggtgcgttac tctgctcagt atgttcctat
    14701 gtttactctg acgctggaca aaaaagaaat taccatgcct aaaaatacgg taatatttcc
    14761 atttgccagc gatgaaacag ctaagtattt aaatgaacaa cagcaaaagt ataaagattc
    14821 gttgtttctg actcgctaat taataaaatt cattacaagg ccaccttcta ataggtggct
    14881 ttttaatttt cggagtatat atgacgtgga aagacaggct tcaggacgcg tcatttcgcg
    14941 gcgtgccgtt taaggttgaa gaagaaagtg cgggaaccgg tcgccgtgtg gaaacacatg
    15001 aatacccgaa ccgcgacaaa ccctataccg aagacctggg gaaaatcact tttcgcccgt
    15061 ccatcacggc ttatgtggtg ggagatgact gctttgacca gcgcgatcgc ctgattgaag
    15121 cgctgaataa accaggtccc ggcacgcttg tccacccgac atatggtgag ctgaaagtct
    15181 gtgttgacgg ggaagttcgg gtcagcacat cgaaaagtga agggcgtatt gtccgctttg
    15241 acctgaagtt tgtcgaagca ggagaactct cttaccccac atcaggtgcg gcgacggcgc
    15301 agacgctgat gtcatcctgt tctgcactgg atgactgcat cagtgacagc ttcagcggtt
    15361 tcagtatcga tggtgtggcg gatttcgtgc agaacgacgt tatcggtaat gccagcataa
    15421 tgctggggta tgtttctgat gcgatgaaag tggtggattc tgccgtatcg gatgccgcca
    15481 ggctgttgca gggggatatc tcggtacttc tgccgccgcc atcgtcaggc aaaaatttcg
    15541 ttgagcaggt gcagaaaatg tggcgtaccg ggaaacgcct ttatggtaac gccagcgacc
    15601 tggtcaccat gatcaaaacg ctttctggtg tcagcctcgg cagcgatttg caaccgcgcg
    15661 gcgtctggaa aacggacagt aaaaccaccg ccacagcgac gcagcagcgt aacatggttg
    15721 ccagcaccct tcgtacgact gcaatcagcg aagcggcgta tgccgttacc cgattgcctg
    15781 cgccaacaac ttctgcggtg atgcagaatg ccgcagtggg gcagtcaaca acacccgcgc
    15841 agagctccgg ctggcctgcc gtcacgcatc cggtgctgaa caatgcaccg gcggtgaaaa
    15901 acacggttga cctgccaacg tgggaagaac tgaccgacat tcgcgacaca ctgaatacgg
    15961 caattgataa ggagttgtcc cgtacaacca gtgatgcgct gtttctggct ctgcgccggg
    16021 tgaaagcaga tctgaatgcg gatatcaaca cgcgccttga acagtctgct cggatcattc
    16081 agcgcacgcc ggatgaggtt ttacccgcgc tggtgctggc ggcgacctgg tttgataacg
    16141 cggcgcgtga cgcggacatt atccggcgta atgccattac gcatcccggc tttgtgccgg
    16201 taatccctct gaaggtgcca gtgcaatgaa cgacaatgtc acgctacggg taaatggccg
    16261 ggagtggaat ggctggacat cggtgcgcat cggtgccggt attgaacggc tggcgcggga
    16321 tttcagcgtg gagatcaccc gccagtggcc gggagatgag ggtatcacca cgcttcagcc
    16381 gcgcattaaa aatggttcaa aagtggaagt gctgattggt gatgagctgg tgatcaccgg
    16441 ctgggtggag gcgacccccg ttcgttacga tgcccgttcg gtcagcaccg gtattgccgg
    16501 acgtagtctg actgctgacc tgattgactg tgcagccgaa ccgacacagt ttaacggacg
    16561 atcgctggta cagattgcgc aggcgcttgc tgcgcctttc ggcattgagg tggtgaacag
    16621 cgatgcgccg tcgggtgtta ttccggatgt ccagcctgat cacggtgaaa cggtgatcga
    16681 ggtgatcaac aaaatactcg gtcagcagca ggcgctggct tatgacgacc cgcacggcag
    16741 gctggtgatt ggtggttttg gctcaacgcg ggcacatacc gcgctggtac ttggggaaaa
    16801 catcctttcc tgtgatacgg agaagagtat ccgggagcgg ttttcagttt accaggtggc
    16861 ggggcagcgt gccggaaacg acgatgattt cggtgaggcc accaccaccg cgctgcgggc
    16921 ccgcacagag gatgcattta ttgcccgtta ccgtccgatg tatatcaggc agacagggca
    16981 ggctacgggg gcaggctgta ttgcgcgtgc tgactttgaa gcccgacaac gggcggcgcg
    17041 gacggatgaa accacctatg tggtgcaggg ctggcgacag ggtaacggta cgctgtggca
    17101 gcccaaccag cgggtgattg tcttcgatcc ggtctgtggt ttcgacaata ccgaactgct
    17161 tgtctcggaa gtcacgttta ctcaggacca gaatggcacc ctgacggaaa tccgtgtcgg
    17221 cccacctgat gcttatctgc ctgaacccga agcccccggc gcgcggaaaa agaaaaaaac
    17281 cagagtacag gaggacccgt tctgatgaag gcgattgaaa ccatacagcg acaactcctc
    17341 ggcctgattg ggcgggcggt ggtgaaaagc atcagtgccg ccacgaaatg tcagaccgtg
    17401 gatgtgtccc tgattgccgg tgaacccaaa gccggggttg aacatcttga accctacggt
    17461 tttaccgcaa gggcaaacag cggtgcggaa gcggtggtgt tgtttccgga tggtgaccgt
    17521 tctcatgcgg tggttgttac ggtgtcggac cgacgctacc gcctgaaagg gctgcagacg
    17581 ggtgaggtgg ctgtctatga cgatcagggg cagtccgtga cgctgacccg ggcggggatc
    17641 gtggtggatg gtgcaggtaa aacgatcacg tttcgcaatg cgcctaaggc tcgttttgaa
    17701 atggacctgg aagtgaccgg acaggtgaaa gacctgtgcg actccagcgg caccaccatg
    17761 tcagcgatgc ggcttgccta taacgggcat cgtcacagag agaacggtca gggcagtaac
    17821 accgacaaac ctgataaagc gatggaggca tgatggaact gtggctgacg gtgaacggta
    17881 aacgcacctg tgccagcgca ccgatggatc cgctgacccg tgccgtggtg atttccctgt
    17941 tcacctggcg gcgggctgaa cctgatgaca atgccgacgt cccgatggga tggtgggggg
    18001 atacctggcc tgcggtacag aatgaccgtt acggctcccg actgtggctg cttcagcgca
    18061 gcaaactgac caatcagctg gtgcagacgg taagggggta tatccgcgaa tgcctgcaat
    18121 ggatgattga tgacggcgtg gtgtcccgta ttgatctgga tatccgccgc accgggatta
    18181 atgaactggg taacagtatc actctctggc gtcgtgacgg accggtaatg atttcttttg
    18241 atgatctgtg gagtgcgata acgcatggcg gacagtgaat ttcagcgccc gacgctggca
    18301 gaaaatatca gtatgctccg taacgattta ttcgccaggc tggacgtcag cgacacgctc
    18361 cggcgcatgg atgaagacgt gcgggcaaag gtgtatgcgg cggcgctgca tacggtttac
    18421 ggttacatcg attatctggc aatgaacatg ctgcctgacc tgtgcgatga gtcctggctg
    18481 gcgcgacatg ctgcgatgaa acggtgtccg cgcaagggag ccacggctgc cagtgggtat
    18541 atgcgctggg aaggtgtcag cgatggcctg aaggtgaccg ccgggagtgt tattcagcgc
    18601 gatgacctgg tgcagtacac gacaactgac gatgcaacca gctccggtgg tgtcctgcgc
    18661 gtgccgatcg cctgctcaag tgcaggtgcg gtcggtaacg ctgacgacgg tacggcatta
    18721 atcctggtca cgccggtgaa tggtctgccg tcttccggtg tggctgacac cctgacaggc
    18781 ggatttgata ctgaagagct ggaaacgtgg cgcgcccgcg tcattgagcg gtattactgg
    18841 acgccgcagg gcggggctga cggggactat gttgtctggg ctaaagaagt gcccggcatt
    18901 acccgcgcat ggacataccg tcactggatg ggaacgggaa ctgtcggtgt gatgattgcc
    18961 agcagtgacc tgattaatcc cattccggaa gaatcaacgg aaacggcggc aagacaacat
    19021 atcgagccac tggccccggt ggcaggctct gatttgtatg tgtttaggcc ggtggcacat
    19081 acggtggatt ttcatatccg cgtgacgccg gacacaccgg aaatacgggc tgccatcacc
    19141 gcggagttgc gttcgttcct gctgcgtgat ggttatccgc agggagaact gaaggtatcg
    19201 cgtatcagtg aggcgatttc cggtgcgaac ggggaataca gccatcagtt gcttgcaccg
    19261 acagacaata tctccattgc aaaaaatgaa ctggcggttc tggggacgat ttcatggacg
    19321 tgacaaacga tgattacatc cgtctgttgt cggcactgtt gccccccggt ccggcgtggt
    19381 cagccagcga tccggcgatt gccggtgcgg caccgtcatt aacccgtgtt catcagcgtg
    19441 cggatgccct gatgcgggag ctggatccgc gcaccaccac tgaactgata aaccgctggg
    19501 agcgtctgtg cggcctgccg gatgaatgta ttccggcagg gacgcagacc cttcgccagc
    19561 gtcagcaacg gctggatgcg aaggttaatc tggcgggcgg catcaatgag gatttttacc
    19621 ttgcacagct tgctgccctg ggcagaccag atgccaacat cacgcgatac gacaaaagca
    19681 cgttcacctg ctcatcggtc tgtactgacg cggtgaatgc gccggaatgg cggtattact
    19741 ggcaggtcaa catgccagcc gccacaaaca ccacctggat gacatgtggc gatccctgtg
    19801 attccgcgct gcgtatctgg ggcgacaccg ttgtcgaatg tgtgcttaac aaactctgcc
    19861 cgtcgcatac ctacgtaatt tttaaatatc cggagtaatc catgcatcgt atagacacga
    19921 aaaccgcgca gaaggataag ttcggcgcgg gtaagaacgg ttttacccgt ggtaaccccc
    19981 agaccggcac acctgccacc gatctggatg atgactactt tgacatgttg caggaggaac
    20041 tttgcagtgt tgtggaggca tccggtgcca gcctagagaa ggggcggcat gaccagctgc
    20101 ttacagcgct tcgtgcgctg ctgttaagcc gcaagaatcc gtttggcgat atcaaatcgg
    20161 atggcacggt gaaaacggct ctcg
//
LOCUS       DAOFQD010000085        19794 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.085, whole genome shotgun sequence.
ACCESSION   DAOFQD010000085 DAOFQD010000000
VERSION     DAOFQD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.085"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            101..562
                     /locus_tag="QBF46_003163"
     CDS             101..562
                     /locus_tag="QBF46_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegJ family protein"
                     /protein_id="HDQ6675313.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIKHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            complement(627..1388)
                     /gene="pphC"
                     /locus_tag="QBF46_003164"
     CDS             complement(627..1388)
                     /gene="pphC"
                     /locus_tag="QBF46_003164"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDQ6675314.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQIAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATNMVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMAGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPLFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWAE"
     gene            complement(1385..2044)
                     /locus_tag="QBF46_003165"
     CDS             complement(1385..2044)
                     /locus_tag="QBF46_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ6675315.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADTKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(2265..2324)
                     /gene="ibsA"
                     /locus_tag="QBF46_003166"
     CDS             complement(2265..2324)
                     /gene="ibsA"
                     /locus_tag="QBF46_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDQ6675316.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(2597..2653)
                     /locus_tag="QBF46_003167"
     CDS             complement(2597..2653)
                     /locus_tag="QBF46_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDQ6675317.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            2932..4179
                     /gene="mdtA"
                     /locus_tag="QBF46_003168"
     CDS             2932..4179
                     /gene="mdtA"
                     /locus_tag="QBF46_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDQ6675318.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRGGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            4179..7301
                     /gene="mdtB"
                     /locus_tag="QBF46_003169"
     CDS             4179..7301
                     /gene="mdtB"
                     /locus_tag="QBF46_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDQ6675319.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQASQYRVVLEHNTENTPGLAALDIIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            7302..10379
                     /gene="mdtC"
                     /locus_tag="QBF46_003170"
     CDS             7302..10379
                     /gene="mdtC"
                     /locus_tag="QBF46_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDQ6675320.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGHATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            10380..11795
                     /locus_tag="QBF46_003171"
     CDS             10380..11795
                     /locus_tag="QBF46_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6675321.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQCLGESP
                     LHMHMVIVSYVLTVAVMLPASDWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVMNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            11792..13195
                     /gene="baeS"
                     /locus_tag="QBF46_003172"
     CDS             11792..13195
                     /gene="baeS"
                     /locus_tag="QBF46_003172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDQ6675322.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRD
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            13192..13914
                     /gene="baeR"
                     /locus_tag="QBF46_003173"
     CDS             13192..13914
                     /gene="baeR"
                     /locus_tag="QBF46_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDQ6675323.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            14105..14437
                     /locus_tag="QBF46_003174"
     CDS             14105..14437
                     /locus_tag="QBF46_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDQ6675324.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIT
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRDSGIASVKANGT
                     SQTVKDNT"
     gene            14584..15945
                     /gene="yegQ"
                     /locus_tag="QBF46_003175"
     CDS             14584..15945
                     /gene="yegQ"
                     /locus_tag="QBF46_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDQ6675325.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEICNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(16276..16593)
                     /locus_tag="QBF46_003176"
     CDS             complement(16276..16593)
                     /locus_tag="QBF46_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675326.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            16999..17898
                     /gene="yegS"
                     /locus_tag="QBF46_003177"
     CDS             16999..17898
                     /gene="yegS"
                     /locus_tag="QBF46_003177"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDQ6675327.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(17980..18753)
                     /locus_tag="QBF46_003178"
     CDS             complement(17980..18753)
                     /locus_tag="QBF46_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6675328.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(18859..>19794)
                     /gene="gatD"
                     /locus_tag="QBF46_003179"
     CDS             complement(18859..>19794)
                     /gene="gatD"
                     /locus_tag="QBF46_003179"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDQ6675329.1"
                     /translation="GLCGSDLSRIFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGD
                     AVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPI
                     EDGAFIEPITVGLHAFHLAQGCENKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISS
                     EKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQLILETAGVPQTVELAVEIAGPH
                     AQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMNYSSPWPGQEWETASRLLTERKL
                     SLEPLIAHRGSFESFAQAVRDIARNAMPGKVLLIP"
BASE COUNT         4667 a         5231 c         5134 g         4762 t
ORIGIN      
        1 taatgcaatt ttatcaggat acaattcaat ttcggcctga taatgattaa cttccacagg
       61 tttttgctaa ctttgactac aaagggattc tggagaaaaa atgaaaaaga tattacttat
      121 gttgagtctg ctatttttta ctaccgcggg ctttagtgaa gtttcggata cgttagtgac
      181 gggaggttat gataaacaag cgatgtctga cgcgattaaa catgcgcgga aagaaaccga
      241 taaattcatc gaggttatga ataaaaaaga tgccgatact ttcgcagtca aagccccgat
      301 aaccgatcac ggaagaaccg agcatttctg gcttacggat gtcacttatt ctaacggcat
      361 gtttattggc gtaatcagca atgaccctgg tattgttaca aacgtagaat atggccagga
      421 gtggaaaatc aagaaagaag atatttcaga ctggatgtat acgcgcgggg ataaaattta
      481 cggcggttat accattgatc ctttattggt cacctatcca aaagaagaag ccgacgagct
      541 aagagcaaaa ttagttcgct aatcagccgt tatatttatt gtttaaactg gcggttgaat
      601 tgttgtcttc aaccgccatg agaaactcac tctgcccaca gcgccagtgc caacgtttta
      661 tcatcatccg tacgctcgtt taccgccggg ctggacaaaa actgctttaa caattcaggt
      721 aataaatcga gttgttcctg cgttgctgcc gccagtccat tgaaaaacgg ggtaaataaa
      781 ggcacatgag gagaattatc cagcatattt aacgccagtc gctggatacc atccgtaaat
      841 gctgcaactt tgtgcgcacg cccagtgctg gtgaaagttt ccagtctgga aacggcatct
      901 tcgtcggtaa tgaagtgcgt catgttggca tattcacccg ccatcggcgt gagaggcaat
      961 tgcagaccgt gacccaaatc taccaccacg ccgccatcgc caatctgcat aatcaaggtg
     1021 ccgtcaggcg atgaaatcag tcccagaaat gtgcaggcaa aatcgcgaac cgctaattct
     1081 ttggcttccg cctcggcaaa aagtcgttga cgaatggtca gtaccatatt tgtggcgagg
     1141 acatcattaa gccccaattc cccgccctgc actttttgcg acatataagc catcgcttca
     1201 ttgacggcga gcatcgcgcc ttcgccaccc tgcgagacac tgcctgcgcc atcagcgaca
     1261 aataccgaca ataatggctg ctggtcattt aaccaggcaa tctgcatctg gcaggcatcc
     1321 tgacaaggca agtccgcgct gatgtgcgac gttccaacgg ttgatgcata gaccagacgc
     1381 cagctcacac tgacgtccag ccttttggcg cttccagcac aacttccgtt cccggcgtgg
     1441 agcgggaaac cgaacgcagc gaactggata accagctaaa cagttcacgg aattgtaatc
     1501 cttgcagagg caaaggctga cgaacgctaa tttgtgccag cgtcttcgta tccgccccct
     1561 gaacaccgat ggaaaagaaa gcaaaccttt tatcttcttc cccccgaaac actttgttgg
     1621 ctgcagcctg ccactcatcg gttggtgcac catcggtaat caggaaaatc cacgggcgat
     1681 aatacgaaat accatttgca cgatactcac gtttccgctc ctctaccata tccagggctt
     1741 tggtaatggc cgcacccatt ggtgtatcgc cctgggcaaa caggatgggc ggaaaaaaat
     1801 tagcggcgct ggtaaagggt tgttccacat gcaccgggcc gaacgtcaca atgccgagtt
     1861 ccactctttt taaagccagc ggatcagcaa gcagttcatc acgaaaggta accaatccgg
     1921 cgttaagttc attaataggt ctaccgttca tagaaccaga aacatccagc agcaaaatgc
     1981 aggggcaacg tggttccggg ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc
     2041 tcataagaaa tcctttctta gagaattatc gaggtccggt atcataccga ctgcaataat
     2101 gaaataagag caaacgaaga aactttcaga attaggcccg gcggcgcaga aaattttgct
     2161 attcgcctcg caagaacgga gagccgtcga ttgacactaa gggcggagtg acataatttc
     2221 aggagtgagg gttagggaga ggtttccccc tccccctggt gttcttagta agcctggaag
     2281 ctaatcacta agagtatcac cagtatgatg acgtgcttca tcataaccct ttccttatta
     2341 aaagccctct tctccgggag aggctttccc gtttcagcgt cccgctgaaa tcatcggctt
     2401 acctcctttc gccctgaatg cagtctatcg ctaacgcgtg aataaaatag tttcctctgt
     2461 gattattgcg taacgactcg caaagaatat ttgctggcag gatcgcagac tacaaagcct
     2521 gcggattgac aatcttatcg tgaaggcata ctttcaggag tgagggtaga gcggggtttc
     2581 ccccgccctg gtagtcttag taagcgggga agcttatgac taagagcacc acgatgatga
     2641 gtagcttcat catgaccctt tccttattta tggccccttc ctcgggaggg gctttcccgt
     2701 ttcagcgtcc cgctgaaatc gtcggcttac ctcctttcgc catgcaagca gtctatcgct
     2761 aacgcgtaga taaaatagtt tcatgtgtta ttactggatg cgtgctcgca aatgtgcccg
     2821 tcattcagac gattccagac agtgtttcat aattcctcca tttttctccc ttattggctg
     2881 gctacactag tatcattccg cgaaacgttt caggaagaga aactcttaac gatgaaaggt
     2941 agttataaat cccgttgggt aatcgtaatc gtggtggtta tcgccgccat cgccgcattc
     3001 tggttctggc aaggccgcaa tgactcccgg agtgcagccc caggggcgac gaaacaagcg
     3061 cagcaatcgc cagcgggtgg tcgaggtggt atgcgttccg gcccattagc cccggttcag
     3121 gcggcgaccg ccgtagaaca ggcagttccg cgttacctca ccgggcttgg caccattacc
     3181 gccgctaata ccgttacggt gcgcagccgc gtggacggcc aactgatagc gttacatttc
     3241 caggaaggcc agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc
     3301 aaagttgcat tagcacaagc ccagggccaa ctggcaaaag ataaagccac gcttgccaac
     3361 gcccgccgtg acctggcgcg ttatcaacaa ctggcaaaaa ccaatctcgt ttctcgccag
     3421 gagctggatg cccaacaggc gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa
     3481 gcaagcgttg ccagcgcgca gctgcaactc gactggagcc ggattaccgc accagtcgat
     3541 ggtcgcgttg gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc
     3601 gggatcgtgg tgatcaccca gacgcatcct atcgatttag tctttaccct gccggaaagc
     3661 gatatcgcta ccgtagtgca ggcgcagaaa gccggaaaac cgctggtggt agaagcctgg
     3721 gatcgcacca actcgaagaa attaagtgaa ggcacgctgt taagtctaga taaccaaatc
     3781 gatgccacta ccggtacgat taaagtgaaa gcacgcttta ataatcagga tgatgcgctg
     3841 tttcccaatc agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg
     3901 atcccaacag ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgaatagc
     3961 gaaaacaagg tcagcaaaca tctggtgacg ccgggcattc aggacagtca gaaagtggtg
     4021 atccgtgcag gtatttctgc gggcgatcgc gtggtgacag acggcattga tcgcctgacc
     4081 gaaggggcga aagtggaagt ggtggaagcc cagagcgcca ctactccgga agagaaagcc
     4141 accagccgcg aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta cccccgagca
     4201 gcacaggcgg cccgtcgcgc ctgtttatta tgcgtcctgt ggccaccacg ctgctgatgg
     4261 tggcgatctt actcgctggg attatcggtt atcgcgccct gcccgtttcg gcgctgccgg
     4321 aagtggacta tccgaccatt caggtggtca cgctctaccc aggtgccagc ccggatgtca
     4381 tgacctctgc cgttaccgcg ccgctagaac gccagttcgg gcagatgtct ggcctgaaac
     4441 agatgtcgtc gcaaagttcc ggcggtgcgt cagttatcac tttgcagttc cagctaacat
     4501 taccgctcga tgtcgccgag caggaagtgc aggccgcgat taacgctgcg accaacttgt
     4561 tgccgagcga tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga
     4621 tcatgacgct cgccgtcacc tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg
     4681 aaacccgcgt cgcgcagaaa atctcgcaga tttccggcgt cggcctggtg acgctttccg
     4741 gcggtcagcg tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc
     4801 tgaccagcga aaccgtgcgc accgccatta ccggcgctaa cgttaactcg gcaaaaggta
     4861 gcctcgacgg cccttcccgt gcggtcacgc tttccgcgaa cgaccagatg caatccgccg
     4921 aagagtatcg ccagctaatc atcgcctacc agaacggcgc gccaattcgt ctgggcgatg
     4981 tcgcaactgt agagcaaggt gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac
     5041 aggccattgt gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca
     5101 gcattcggca gatgctgcca cagctcactg agagtctgcc gaaatcggtg aaggtgacag
     5161 tgctttccga tcgcaccacc aatatccgcg catccgtcga tgatactcag tttgaattga
     5221 tgatggctat cgcgctggta gtcatgatta tctacctgtt tttgcgcaat attccggcga
     5281 ccatcattcc cggtgttgct gtaccgctgt cgttaatcgg cactttcgcg gttatggtgt
     5341 ttctcgattt ttcaatcaat aacctgacac tgatggcgtt aactatcgcc accggattcg
     5401 tggtcgatga cgccatcgtg gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa
     5461 aaccgttggc ggcggcgctc aagggcgcag gtgaaatcgg ctttaccatt atctcgctga
     5521 ccttctcact gattgcggtg ttgatcccac tgctgtttat gggcgatatc gtcgggcgac
     5581 tgttccgcga atttgctatt accctggcgg tagcgatttt gatctcagcg gtggtgtcgc
     5641 tgaccctgac accgatgatg tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga
     5701 accgcttctc ccgtgcctcg gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg
     5761 gactggcgaa agtgctgaat catccgtggc tgaccttaag cgtggcactc agcacgctgc
     5821 tgcttagcgt gctgctgtgg gtgttcattc cgaaaggttt cttcccggta caggacaatg
     5881 gcattattca gggcactttg caggcaccgc aatccagctc ctttgccaat atggcccagc
     5941 gacaacgcca ggtcgcggac gtgattttgc aggatccggc agtgcaaagc ctgacctcat
     6001 ttgttggcgt tgatggcact aacccgtcgc tgaacagtgc acgtttacaa atcaacctca
     6061 aaccgttgga tgaacgtgat gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg
     6121 tagataaagt gccgggcgtc gatctcttcc tgcaaccaac gcaggacctg accatcgata
     6181 ctcaggtcag ccgcacccag taccagttta ccttgcaggc cacctcactg gatgcgctca
     6241 gtacctgggt gccacagttg atggaaaaac tccagcagct gccacagcta tctgatgtct
     6301 ccagcgactg gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca
     6361 gccgtctggg gatcagcatg gcggacgtcg ataacgccct gtacaacgcg tttggtcagc
     6421 ggctgatttc cactatttat actcaggcca gccagtatcg cgtggtgctg gagcacaaca
     6481 ccgaaaatac cccaggcctc gcggcgctgg atatcattcg cctgaccagc agcgacggcg
     6541 gcgtggtgcc gctaagctca attgccaaaa ttgagcagcg ttttgcgccg ctctccatca
     6601 accatctgga tcagttcccg gtaacgacca tctcctttaa cgtgccggat aactattcgc
     6661 tgggcgatgc ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata
     6721 tcaccacgca gttccagggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct
     6781 ggctgattgt cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct
     6841 ttattcaccc gatcaccatt ctctcgacgc tacccaccgc aggagtcggc gcactgctgg
     6901 cgttaatgat tgccggtagc gaactggatg tgattgcgat tatcggcatt attttgctga
     6961 tcggtatcgt gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg
     7021 agcaaggcat gtcgccgcgc gatgccatct accaggcttg tctgttgcgt tttcgtccga
     7081 tcctgatgac cactctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg
     7141 tcggcgcgga actgcgtcgt ccgttaggta tcggcatggt cggcggcctg attgtcagcc
     7201 aggtgctgac gctgtttacc acgccggtga tttatctgct gttcgaccgc ctggcattgt
     7261 ggaccaaaag tcgctttgct cgccatgaag aggaggcgta agtgaagttt tttgccctct
     7321 tcatttaccg tccggtggcg acgattttac tgtcggttgc cattaccctg tgcggcatac
     7381 tgggcttccg tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg
     7441 tcagcgcctc gctgcccggt gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc
     7501 tggagcgctc acttgggcgc attgccggag tcagtgaaat gacctccagt agttcgctcg
     7561 gcagcacgcg cattattttg cagtttgatt ttgaccggga tatcaacggc gcagcgcgtg
     7621 atgtgcaggc ggcgatcaac gctgcacaaa gtttgctgcc cagcgggatg cccagccgcc
     7681 cgacctatcg caaagcgaac ccgtcggatg cgccaattat gatcctcacg ctgacatccg
     7741 atacttattc gcagggtgaa ctgtacgatt ttgcctcgac gcagctggct ccgacgattt
     7801 ctcaaatcga cggcgtcggt gatgtcgatg tcggcggcag ttcgctgccc gccgtgcgcg
     7861 tcgggctgaa cccgcaggca ctgtttaatc agggcgtgtc gctggacgac gtacgcactg
     7921 ccatcagcaa tgccaacgtg cgtaaaccgc agggggcgct ggaagatggc actcaccgct
     7981 ggcagatcca gaccaacgat gaactgaaaa ccgccgctga atatcagcca ctgattattc
     8041 actacaacaa cggcggcgcg gttcgtctgg gcgatgtggc gacggtgacc gactcagtgc
     8101 aggatgtgcg caacgccggg atgaccaacg ccaaaccggc tattttgctg atgatccgca
     8161 agctgccgga agccaatatt atccagacgg tagacagcat ccgggcaaaa ttaccggagt
     8221 tgcaggaaac cattccggcg gcgattgatc tgcaaattgc tcaggatcgc tcccccacca
     8281 ttcgcgcctc gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc
     8341 tggtggtgtt tttattcctg cgctcgggtc acgcaactat tattcccgcc gttgcggtgc
     8401 cggtttcgct gattggtacg tttgcggcga tgtacctgtg cggtttcagt ctcaataatc
     8461 tttcgttaat ggcgctcacc atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc
     8521 tggaaaacat tgcccgtcat ctggaagcgg gaatgaaacc gttgcaagcc gcactgcaag
     8581 gtactcgcga agtcggtttt acggtgctgt cgatgagtct gtcactggtg gcggtgttcc
     8641 tgccgctgct gttgatgggc ggattgccgg gccgactgtt acgcgagttt gccgtgacgc
     8701 tttctgtcgc cattggtatt tcgttgctgg tttccctgac gctaacgcca atgatgtgcg
     8761 gctggatgct aaaagccagc aagccgcgtg agcaaaagcg actgcgtggt tttggccgca
     8821 tgttggtagc cctgcaacaa ggctacggca agtcgctgaa atgggtgctc aatcataccc
     8881 gtctggtggg tgtggtgctg cttggcacca ttgcgctgaa tatctggctg tatatctcga
     8941 tcccgaaaac cttcttcccg gaacaagaca ccggcgtgct gatgggcggg atacaggcgg
     9001 atcagagtat ttcgtttcag gcgatgcgcg gtaagttgca ggattttatg aaaattatac
     9061 gtgacgatcc ggcagtggat aatgtcaccg gctttaccgg cggttcacga gttaacagtg
     9121 ggatgatgtt tatcaccctc aagccacgcg acgaacgcag cgaaacggcg cagcaaatta
     9181 tcgaccgtct gcgcgtaaaa ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg
     9241 tacaggatat tcgcgttggt gggcgtcagt cgaacgccag ctaccagtac acgttgttat
     9301 ccgacgacct ggcggcactg cgtgaatggg agccaaaaat ccgcaaaaaa ctggcgacgt
     9361 tgccggaact ggcggacgtg aactccgatc agcaggataa cggcgcggag atgaatctgg
     9421 tttacgatcg cgacaccatg gcgcggctgg gaatcgacgt gcaggccgcc aacagcctgt
     9481 taaataacgc cttcggtcag cggcaaatct cgaccattta ccagccgatg aaccagtaca
     9541 aagtggtgat ggaagtggat ccgcgctata cccaggacat cagcgcgctg gaaaaaatgt
     9601 tcgttatcaa taacgaagga aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg
     9661 cgaatgcccc actatcggtg aatcatcagg gattatcggc ggcctcgacc atttcgttta
     9721 acctgccgac cgggaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc
     9781 agcttggcgt gccttcgacg gtgcgcggca gttttgccgg cacagcgcag gtgttccagg
     9841 agacgatgaa ctcgcaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc
     9901 tgggtatcct ttacgagagt tacgtacatc cgctgacgat cctctccacc ctgccctcgg
     9961 cgggcgttgg agcgctgctg gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc
    10021 tgatagggat catgctatta atcggcatcg tgaagaaaaa cgccattatg atggtcgatt
    10081 ttgcgcttga agcccaacgg cacggtaacc tgacgccgca ggaagctatt ttccaggcct
    10141 gtctgctgcg ttttcgcccg attatgatga ctaccctggc ggcgctgttt ggcgcgctgc
    10201 cgctggtgtt gtcgggcggt gacggctcgg agctgcggca acccctgggg atcaccattg
    10261 tcggcggtct ggtgatgagt cagctcctga cgctgtatac cacgccggtg gtatatctct
    10321 ttttcgaccg tctgcggctg cgtttttcgc gtaaacctaa acaaacggta accgagtaaa
    10381 tgacagatct tcccgacagc acccgctggc aattgtggat tgtggctttc ggcttcttta
    10441 tgcagtcgct ggacaccacc atcgtaaaca ccgcccttcc ctcaatggcg caatgcctcg
    10501 gggaaagtcc gttgcatatg cacatggtca ttgtctctta tgtactgacc gtggcggtga
    10561 tgctgcccgc cagcgactgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg
    10621 ctatcgtgct gtttactctc ggttcattgt tttgcgcgct ttccggcacg ctgaacgaac
    10681 tgttgctggc acgcgcgtta cagggcgttg gcggtgcgat gatggtgccg gtcggcaggt
    10741 tgacggtgat gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt
    10801 tacccggtca ggtcggtccg ctgctcggtc cggcgctcgg cggtctgctg gtagagtacg
    10861 cgtcgtggca ctggatcttt ttgatcaaca ttccggtggg gattatcggt gcgatcgcca
    10921 cattgatgtt aatgccgaac tacactatgc agacgcggcg ctttgatctt tccggctttt
    10981 tattgctggc ggttggcatg gcggtattaa ccctggcgct ggacggcagt aaaggtacag
    11041 gtttatcgcc gctggcgatt actggcctgg tcgcagttgg tgtggtcgca ctggtgcttt
    11101 atctgctgca cgccagaaat aacaaccgcg ctcttttcag tctgaaactg ttccgcactc
    11161 gtaccttttc gctgggcctg gcggggagct ttgccggacg tatcggtagc ggcatgttgc
    11221 cctttatgac gccggttttc ctgcaaattg gccttggctt ctcaccgttt catgccggac
    11281 tgatgatgat cccgatggtg cttggcagca tggggatgaa gcggattgtt gtacaggtga
    11341 tgaatcgctt tggttatcgt cgggtactgg tagcgaccac gctgggtctg tcgctggtca
    11401 ccctgttgtt tatgactacc gctctgttgg gctggtacta cgttttgccg ttcgtcctgt
    11461 ttttacaagg gatggtcaac tcgacccgtt tctcctccat gaacaccctg acgctgaaag
    11521 atctcccgga caacctggcg agcagcggca acagtctgct gtcgatgatt atgcaattgt
    11581 cgatgagtat cggcgtcacc atcgccgggc tgttgctggg actttttggt tcgcagcatg
    11641 tcagcgtcga cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt
    11701 ttatcatcgc ccttccggcg ttcatctttg ccagagtgcc gaacgatacg catcaaaatg
    11761 tagctatttc gcggcgaaaa aggagcgcgc aatgaagttc tggcgacccg gtattaccgg
    11821 caaactgttt ctggcgattt tcgccacctg cattgtcttg ctgatcagta tgcactgggc
    11881 ggtgcgtatc agttttgagc gtggctttat tgattacatc aagcatggta atgaacagcg
    11941 attacaactg ttaagtgatg cgcttggcga gcagtatgcg cagcatggta actggcgctt
    12001 cctgcgcaac aatgatcgct ttgtctttca gatcctgcgt tcatttgaac acgataattc
    12061 ggaagataaa cccggcccgg gtatgccacc gcacggctgg cgtacccagt tctgggtggt
    12121 tgatcaaaac aacaaagtgc tggttggtcc gcgagcgccg attccacctg acggtacacg
    12181 gcgacccatt ctggtcaacg gtgcggaagt gggcgcggtg attgcctccc ctgttgaacg
    12241 gctgacccgc aatactgata tcaatttcga taaacaacag cggcaaacca gctggctgat
    12301 tgtcgccctg gcaacgttac tcgctgcgct cgccaccttt ctgctggcgc gtggtctttt
    12361 ggccccggtc aaacggctgg tggacggcac gcacaaactg gcggcgggcg atttcaccac
    12421 tcgcgtgacg cccaccagtg aagatgagct gggcaaactg gcgcaagact tcaaccagct
    12481 cgccagcacg ctggagaaaa accagcaaat gcgccgcgat tttatggccg atatttctca
    12541 cgaattgcgt acaccactag cggtacttcg cggtgagctg gaagctattc aggatggcgt
    12601 gcgtaaattc acgccggaga cggtggcctc tttgcaggca gaagtcggaa cgctaaccaa
    12661 actggtggac gatctccatc agttatcgat gtcagatgaa ggcgctctcg cctatcaaaa
    12721 agcaccggta gatctgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt
    12781 cgccagccgt gacctgaaac tgcaattttc cctgccagac agtattaccg tatttggcga
    12841 tcgcgaccgt ttaatgcagt tgttcaataa cttactggaa aacagcctgc gctacactga
    12901 cagcggcggc agcctgaaaa tctctgccga gcagcacgac aaaacggtgc gcctgacctt
    12961 tgctgacagt gcgccaggtg tcagtgacga tcagctacaa aaattgtttg aacgttttta
    13021 tcgcaccgaa ggttcccgca accgtgccag cggcggttcc gggctggggt tggcgatttg
    13081 cctgaacatt gttgaagcac ataatggccg cattattgct gcccattcgc cttttggcgg
    13141 ggtaagcatt acagtagagc taccgctgga acgggattta cagagagaag tatgaccgag
    13201 ttaccaatcg acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg
    13261 cagttgctca ttgattatct gcgtgctgcg agctatgcgc cgacgcttat cagccacggc
    13321 gatcaggtac tgccgtatgt gcgccagaca ccaccggatc tgatcctgtt ggatctgatg
    13381 ctccctggca ccgatggctt gacgctgtgc cgggaaatac gtcgtttttc tgacattcct
    13441 atcgtgatgg tgacagcaaa aatcgaagag atcgaccgcc tgctggggct ggagattggc
    13501 gcagatgact acatctgcaa gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc
    13561 attttgcgcc gttgcaaacc gcagcgcgag ttgcagcaac aggatgctga aagtccgttg
    13621 attatcgacg aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacgcct
    13681 gcggaatttc gtctgctgaa aacgctctct catgaaccag gaaaagtgtt ctcccgcgag
    13741 caattgctca atcatcttta tgacgactac cgcgtagtaa ccgaccgcac catcgatagc
    13801 cacattaaaa acctgcgccg caagctggaa tctctcgacg ccgaacagtc atttatccgc
    13861 gccgtttatg gcgtcggtta ccgctgggaa gccgacgcct gccgcatcgt ttagttttag
    13921 cgacatgact ttgttagccg gagatggcat tcagaccaga tccggcaaca ttatcccacg
    13981 catggtcagc aaactgacat tatctcttct ccctggcaac cgcctaaaaa tcgttcataa
    14041 tttaatcggt aactagtgct gccagacgct gttttcgata cacaatgtca atcaaggagt
    14101 cattatggct ggttggtttg aactcagtaa gagcagtgat aatcagttcc ggtttgtgct
    14161 aaaagcgggc aatggtgaga cgatcctcac cagcgagctg tatacctcaa aagcctctgc
    14221 ggaaaagggc atcacgtcgg tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa
    14281 aacggcaagc aacggcaaat tctatttcaa tctgaaagcc gccaatcatc aaattatcgg
    14341 ctccagccag atgtatgcca ccgcgcaatc ccgcgacagc ggaattgcct ccgttaaagc
    14401 caatggcaca agccagacgg tgaaagacaa tacgtaatta cgatgccggg cgagggttgt
    14461 cggcgtccgg cgctttacca gatcccccca cagcgctaca atgcccgccc ttaaagtggg
    14521 ggcactcccc taaccgcttc atcaggtgaa gcggatctga cctgtcatca gaacgagaga
    14581 attatgttta aaccggaact cctttccccg gcgggaacgc tgaaaaatat gcgttacgct
    14641 ttcgcttatg gcgcagatgc tgtttatgcg ggccagccgc gttactccct gcgtgtgcgc
    14701 aacaacgaat tcaaccacga aaatcttcag ctcggcatca atgaagccca cgcgctgggg
    14761 aaaaagtttt atgtcgtggt caacattgca ccgcacaacg ccaagctgaa aacctttatc
    14821 cgtgacctga aaccggtggt ggaaatgggg ccggatgcgc tgattatgtc cgatccaggg
    14881 ctgattatgc tggtgcgtga gcacttccct gaaatgccga tccacctctc ggtgcaggct
    14941 aacgccgtaa actgggcgac ggtgaaattc tggcagcaaa tgggcctgac ccgcgtgatc
    15001 ctctctcgcg agctgtcgct ggaagagatt gaagagatct gcaatcaggt gccggatatg
    15061 gagatcgaga tcttcgttca cggtgcgctg tgcatggcct actccggtcg ctgcctgctc
    15121 tctggctata tcaacaagcg cgatccgaac cagggtacct gcaccaacgc ctgccgctgg
    15181 gagtacaacg tccaggaagg gaaagaagat gacgttggca acatcgtaca caagtacgag
    15241 ccgattccgg tgcaaaatgt tgagccgacg ctaggtatcg gcgcgccaac cgacaaagtg
    15301 tttatgatcg aagaagccca gcgtccgggc gagtatatga ccgcgtttga agatgagcac
    15361 ggcacttaca tcatgaactc gaaagatctg cgcgccatcg cccatgtaga acgcctgacc
    15421 aaaatgggcg tgcattcgct gaaaatcgaa ggccgtacta aatctttcta ctattgcgca
    15481 cgcaccgcac aggtttatcg taaagctatc gatgatgccg ctgcgggcaa accgttcgat
    15541 accagcctgc tggaaactct ggaaggtctg gcgcatcgtg gctataccga aggtttcctg
    15601 cgtcgtcata ctcacgacga ttatcagaac tacgaatacg gttattcagt ttctgaccgc
    15661 cagcagtttg ttggtgagtt taccggtgag cgcaaggggg acctcgcagc ggtagcggtg
    15721 aaaaataaat tctccgttgg cgacagcctt gagctgatga cgccgcaagg caacattaac
    15781 tttacccttg agcacatgga aaacgccaaa ggtgaagcga tgccggtcgc accaggcgat
    15841 ggttatactg tgtggctccc ggtcccgcag gatcttgagc tcaattacgc tctgctgatg
    15901 cgtaatttct ccggggaaac cacgcgtaac ccacacggta agtgattaat ttcgattatt
    15961 tttcccgtat ggaaaattct tagaaaccga tcacatacag ctgcatttat taaggttatc
    16021 atccgtttcg ctgaaaaaca taacccataa aatgctagct gtaccaggaa ccacctcctt
    16081 agcctgtgta atctccctta cacgggctta tttttttatg cataagccct atctctggta
    16141 accgtcttcc attgaccaca tcgatagaat cctccttcat agcacgatgc ctttcactta
    16201 tcggcatcgt gctcccacag gttccggcta cgcacagcca gaacgcgcat atttgacgct
    16261 taccaaaaaa tattctcact ctccacattt gaatgtcaga cgagcgacac catgtaatcc
    16321 tacaccttct gtcttcagct caactatttg catttttttg ccctgagtaa cacagaaatg
    16381 agttgcatca ttttttacta tattttctgc accagatatt ctacccctgg ctaaagaagc
    16441 ttcggcttcg gtgtagtatt ggttatcgag tttacgctga atattacttt tatatgcaag
    16501 accaaattta ccgatacttg tctcatcatt atgcacagca caaccagaca taataaaaat
    16561 actaattaat gatatagcag ctatcttttt catctcacct tcccccatta aataccaaca
    16621 acactctcta gtgtttaaat ataataatgg catgattatt ataattgaat aggattataa
    16681 taaatgttct gtacaacatt tcctacataa gtaggaatta cggacattga ggcccttcag
    16741 ggtaactcca tgggggcttt aatatattat attgaagatg ccactgttta gttgaatatt
    16801 aggtatatgc tcttttttga aatttatcgg tggcagccgt tagtattcgc tgtccccatt
    16861 gcaagctcct ggtggtaacc actgaatcct ccatacttga actgactttt tatcctccga
    16921 ctttcatcct gttctgactc caccttttgt tttctgctct acactatcta cagaccaatc
    16981 ataaaggcac atacgatcat ggcagaattt cccgccagct tactgattct taatggcaaa
    17041 agtactgaca atctaccctt gcgcgaagca attatgctgt tgcgtgagga aggaatgacg
    17101 atccatgtgc gggtcacctg ggagaaaggc gatgccgcac gatatgtaga ggaggcccgg
    17161 aagttgggcg tcgcaacggt gattgccggt ggtggcgatg gcaccattaa tgaagtttct
    17221 acggcgttga ttcagtgtga gggggatgac atacccgcgc tgggaatttt gccattagga
    17281 accgccaatg attttgccac cagtgtaggg attcctgagg cactggataa ggcgctgaaa
    17341 ctggcaattg ccggtaacgc cattgcgata gatatggcgc aggtcaacaa acaaacctgt
    17401 tttattaata tggcgacagg cggatttggg acgcgtatta ccacagaaac gccggaaaaa
    17461 ttaaaagccg cgctgggtgg cgtctcttac atcattcatg gcttaatgcg catggatact
    17521 ctgcaaccgg accgttgtga aatccgcggt gaaaactttc actggcaagg tgacgccctg
    17581 gtcattggta ttggtaacgg gcgtcaggcc ggtggcggtc agcaattgtg tccgaacgcg
    17641 ttaattaacg atggcttgct gcaactgcgc atttttaccg gcgatgaaat tcttccggct
    17701 ctcgtatcaa ccttaaaatc tgacgaagat aacccgaata ttatcgaagg cgcttcgtcg
    17761 tggtttgata tacaagcccc acacgaaatc acttttaatc ttgatggcga accgttgagt
    17821 gggcaaaatt ttcatattga aatacttccg gcggcgttac gttgtcgatt accaccagat
    17881 tgtccactgt tgcgttaatc aaactattct ctcttgaacc agggagaatc cgtaatgccg
    17941 gatgcggtgt aaacacctta tccggcatac agaacaatac tacaccgtaa ttaattttac
    18001 ccgcgtcttc tgcaatgcca gtttatccac ctctgacaca gcatcatcgg taataataat
    18061 gtcaattttt tcgattggta gtacctgatt aaaaccgcga cggttaaatt tcgatgaatc
    18121 gagaacggca atcactttat tagcggctgt gaccatcgca ccgctaatag aataaccttc
    18181 attaaaggtc gtaataccat taaccgcatc aataccatca gcaccgacaa acattaaatc
    18241 ggcattaata tcctgtaatg aacgctcggc aatggaacca tgcatcgagc gcgttttatg
    18301 gcggaccgtg ccaccacaga cgaccagagt aatgtcttta ttttcggaaa gggcaaacgc
    18361 tgccgggaga ctgttggtaa tcaccgtgat atttttggcg gtcattaatc cttcggcgat
    18421 aagcattgtc gtacttccgc tgtcgagaat aacggtcatc ccttcgtgga tcattttgac
    18481 tgccgcctgc gctattctgt tttttggcgc gctggcgagc tgaaatcgct ctttaaaccc
    18541 gacttcctgg gtctcggttt cactattacc agacattatt ttcgccgcac cgccatgaaa
    18601 gcgcgtaacc acgccttttt gttcgagaaa gcgcaaatcg gcacggattg tcgcttccga
    18661 ggcagcaaat actcccgcca gatcctgaac aagcacggtt ccctgttcat tcactaattg
    18721 gatgatctta ttccttcgct cgaatgaatt catcgtcatt tacccatatt caattgtggc
    18781 tagtgtaaac gaagggggac cattggtgaa tgatctgttt tgcacaatac cgggccagca
    18841 tcacgctggc ccgcggtttc agggaatgag caacactttg cccggcatag cattacgagc
    18901 gatgtcacgc accgcctggg cgaagctttc aaagcttcca cggtgagcga ttaatggctc
    18961 caggcttaac ttacgttctg tcagcaaccg gctcgccgtt tcccactcct gccccggcca
    19021 agggctggag tagttcatcc aactgccgat aaccgtcagc tctttacgca atattttgcc
    19081 aaacgttgcc gatgttaaat gcagatcctg atgcaacgtg cccaccagcg ccagttgggc
    19141 atgaggaccg gcaatctcta ccgccagttc gacagtttgc ggtacgccag ccgtctcgag
    19201 gataagctga ttaaagcgca gttcgcgtaa aacgctctgc atttgcggcg cgctcatttc
    19261 gctactgtta aatgtttgca tcgcaccgaa agattttgcc agtgccagtt tttctgaact
    19321 aatgtcgatc gccgtcacac tctttgctcc cagcgcgaca gcgcactgaa tggccagcag
    19381 gccaatggtt ccggcaccaa taataataac gtttttattc tcacaacctt gcgctaaatg
    19441 aaaagcatgc aggccaacgg taatcggctc aataaaagcc ccatcctcaa taggcatatc
    19501 cgtgggtaga gcaaagacat tttttcgctt aacgacaata tattcagcaa atccaccatc
    19561 acgccgcgag ccaataaaat catattttgc gcactgggaa taaaaccctt tcagacactc
    19621 tggacaagta aaacagggta ataacggcac acaggcaacc gcatcgccag ggtgtaaatc
    19681 atcaacaccg gatcccaccg catcaatata gccgctaaat tcatggccta acgttattgg
    19741 ataataatgt gcaccatttt taaatatcct ggataaatcg gaaccacata agcc
//
LOCUS       DAOFQD010000086        19530 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.086, whole genome shotgun sequence.
ACCESSION   DAOFQD010000086 DAOFQD010000000
VERSION     DAOFQD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.086"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1173
                     /locus_tag="QBF46_003180"
     CDS             <1..1173
                     /locus_tag="QBF46_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDQ6675330.1"
                     /translation="FGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRL
                     ILATVMACCGFFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLW
                     RHFRQHLAVRWRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWN
                     YQKPDQRRIKIALPYVVGAGSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLG
                     AITTGKDSQGKLPPAVYWLTLHWRIGMWLSMRWFCLRLEPVSKITAGVYLGAFPRHIP
                     AQNAVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLV
                     HCALGLSRSALVAAAWLLCYGHCKTVNEAISYIRARRPQIVLTDEHKAMLRLWENR"
     gene            complement(1227..1832)
                     /gene="azoR"
                     /locus_tag="QBF46_003181"
     CDS             complement(1227..1832)
                     /gene="azoR"
                     /locus_tag="QBF46_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDQ6675331.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVSA"
     gene            2033..5935
                     /gene="hrpA"
                     /locus_tag="QBF46_003182"
     CDS             2033..5935
                     /gene="hrpA"
                     /locus_tag="QBF46_003182"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDQ6675332.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRQRFLRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHYNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            6207..7007
                     /locus_tag="QBF46_003183"
     CDS             6207..7007
                     /locus_tag="QBF46_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDQ6675333.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTAGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            7204..8643
                     /gene="aldA"
                     /locus_tag="QBF46_003184"
     CDS             7204..8643
                     /gene="aldA"
                     /locus_tag="QBF46_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDQ6675334.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFETMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(8685..9686)
                     /gene="gap"
                     /locus_tag="QBF46_003185"
     CDS             complement(8685..9686)
                     /gene="gap"
                     /locus_tag="QBF46_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDQ6675335.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            9875..10405
                     /gene="cybB"
                     /locus_tag="QBF46_003186"
     CDS             9875..10405
                     /gene="cybB"
                     /locus_tag="QBF46_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDQ6675336.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            10650..10823
                     /locus_tag="QBF46_003187"
     CDS             10650..10823
                     /locus_tag="QBF46_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675337.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(10895..11044)
                     /gene="hokB"
                     /locus_tag="QBF46_003188"
     CDS             complement(10895..11044)
                     /gene="hokB"
                     /locus_tag="QBF46_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDQ6675338.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            11443..13083
                     /gene="trg"
                     /locus_tag="QBF46_003189"
     CDS             11443..13083
                     /gene="trg"
                     /locus_tag="QBF46_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDQ6675339.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(13121..14044)
                     /locus_tag="QBF46_003190"
     CDS             complement(13121..14044)
                     /locus_tag="QBF46_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6675340.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            14261..15604
                     /locus_tag="QBF46_003191"
     CDS             14261..15604
                     /locus_tag="QBF46_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDQ6675341.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     LLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            15829..17484
                     /gene="opgD"
                     /locus_tag="QBF46_003192"
                     /gene_synonym="mdoD"
     CDS             15829..17484
                     /gene="opgD"
                     /locus_tag="QBF46_003192"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDQ6675342.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            17624..17848
                     /locus_tag="QBF46_003193"
     CDS             17624..17848
                     /locus_tag="QBF46_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDQ6675343.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            17911..18447
                     /gene="rimL"
                     /locus_tag="QBF46_003194"
     CDS             17911..18447
                     /gene="rimL"
                     /locus_tag="QBF46_003194"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDQ6675344.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(18442..19422)
                     /locus_tag="QBF46_003195"
     CDS             complement(18442..19422)
                     /locus_tag="QBF46_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675345.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVKGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINVEERITRTPLAGLL"
BASE COUNT         4919 a         4646 c         5078 g         4887 t
ORIGIN      
        1 ttcggttggg aaacggcgat cccttttctt ccctggacta ttgttcctta ctggagtctg
       61 gatcttttat atggattttc gctgttcgtt tgtagctcga cattcgaaca gcgccgactt
      121 gtccaccggc ttattctggc aacggtaatg gcctgctgcg gtttttttct ctatccgctg
      181 aagtttagtt ttatccgtcc tgaagtgagt ggggtgacag gatggctatt ttcgcaactt
      241 gaactgtttg atctgcctta taaccagtct ccttcgctgc atattattct ctgctggcta
      301 ctttggcgtc actttcgtca gcatctggct gtgaggtggc gtaaagtctg cggcggatgg
      361 tttttactca tcgccatttc gacgctgacg acctggcagc atcattttat tgatgtcatc
      421 acggggctgg cggtaggtat gttaattgac tggatggtgc ccgtcgaccg tcgttggaat
      481 tatcagaaac ctgatcaacg tcgaatcaaa atagcactgc catatgtcgt aggcgcgggc
      541 tcgtgcattg tgttgatgga gctaatgata atgcttcagt tatggtggtc agtctggtta
      601 tgttggccag tattatcgct attcatcatt ggccgtgggt acggtgggct tggcgcgata
      661 acaacaggga aagatagtca ggggaaactc ccgcccgccg tttactggct gacattgcac
      721 tggcgtatcg ggatgtggct gtctatgcgt tggttttgtc ttcgcctgga gccggtgagc
      781 aaaattactg ctggtgttta tttaggggcg tttccacgac atattccggc acagaatgcg
      841 gttctggacg tcacctttga attccctcgc ggacgagcca caaaagatcg actctatttt
      901 tgtgtaccga tgctggatct ggtggttccg gaagaggggg agctccgaca ggccgtggcg
      961 atgctggaaa cattacgcga agagcaaggc agcgttctgg tccattgtgc attgggatta
     1021 tcgcgcagtg cgctggtggc ggcggcatgg ttgttatgtt acggacactg taaaaccgtt
     1081 aatgaagcga ttagctatat tcgagccaga cgcccgcaga ttgtgctgac agacgagcac
     1141 aaagcgatgc tgagattatg ggaaaacagg taagtggatt gagatgtgga ctgaatatct
     1201 acagtccaca tcaagaccgt gtccggttat gcagaaacaa tgctgtcgat ggctgctttt
     1261 gcgtcagact gtgctttcgc tgccatttcc ggaccgtatg cgatcccttc ggcgaagaca
     1321 aatttcacat cggtaatgcc gataaagccg aggaacgtgg acagatacgg cgtcaccagg
     1381 tccgttggtc catctttgtg gatcccgccg cggctggtaa taacgatggc ttttttaccc
     1441 gttaccagac cttccggacc gttctcggta tagcggaaag taacgcctgc acgtgcaacc
     1501 aggtcaaaat agtttttcag ctgagtcgag atgttgaagt tgtacatcgg tgccgcaata
     1561 acgataacgt cgtgggcttt cagctcggca atcagctcat cggaaagcgc cagagcttcc
     1621 tgctgacgcg gagtcagcgg cgcatcgctc ggacgcagtg caccaaccag ttcgccatcc
     1681 agtaccggaa tcggatttgc agccaggtcg cgaacggtga tttcatcagc ggagtgcttt
     1741 tcgcgccatt gttcaacaaa ataatcggac aactgattag actgagagta ccctgccagg
     1801 atgctggatt taagaactaa taccttgctc atggtgtttc cttatagata tttgaatggg
     1861 cgatgccccg ttgcttgttg acactttatt cacaatcctg ccacagagat agcgcaataa
     1921 atcgaagcct atgttcgaat ttattgaaca acgcatagaa agccgcgatg tggtactcta
     1981 tatctatcat ttaaaagaaa attaatcagg cagcctactg ccctctaacg ttatgacaga
     2041 acaacaaaaa ttgaccttta cagccttgca gcagcggctg gattcgctga tgctgcgtga
     2101 cagacagcgt tttttacgcc gtctgcacgg tgtgaagaag gttaaaaatc ctgatgcaca
     2161 acaggccatt ttccaggaga tggctaaaga gattgaccag gcggcaggga aagtcctgct
     2221 gcgtgaagcg gcacgaccgg aaattactta tcctgacaat ttaccggtta gtcagaaaaa
     2281 gcaggacatt ctcgaagcga ttcgtgatca ccaggtggtg atcgtcgccg gggaaaccgg
     2341 ttccggtaaa acgactcagc taccgaaaat ctgtatggag ctggggcgcg ggattaaagg
     2401 gctgatcggc catacccagc cgcgtcgtct ggcggcaaga acagtggcga accgtattgc
     2461 ggaagagctg aaaacggagc cgggcggttg catcggttac aaagtgcgtt tcagcgatca
     2521 cgtaagtgat aacacgatgg tcaagctgat gaccgacggt atcctgctgg cggagatcca
     2581 gcaagaccgc ctgctgatgc agtacgacac tatcattatt gacgaagcgc acgaacgcag
     2641 cctgaatatc gattttttgc tcggctattt gaaagagttg ctgccgcggc gtcctgacct
     2701 aaaaatcatt atcacttccg cgactatcga cccggaacgc ttttcgcgcc actataataa
     2761 tgcgccgatt attgaagtct ccggtcggac ctatccggtg gaagtgcgct atcgcccgat
     2821 tgttgaagaa gccgatgaca ccgagcgcga tcaattgcag gcgatttttg acgccgtaga
     2881 cgaactgagc caggaaagcc ccggcgacat tttgatcttt atgagcggcg agcgggaaat
     2941 tcgcgatacc gccgatgcgc tgaacaagct aaatctgcgt cataccgaaa tcttgccgct
     3001 ttatgcgcgg ctttcgaaca gcgaacagaa ccgcgtgttc cagtcgcaca gtggacggcg
     3061 tattgtgctg gcgaccaacg tcgcggaaac ctcgctgacc gtaccgggga ttaaatacgt
     3121 tatcgacccc ggtacagcac gtatcagccg ctacagctat cgcaccaaag tgcagcgttt
     3181 gccgattgag cccatttcgc aggcgtctgc caatcagcgt aaaggccgct gtggtcgtgt
     3241 gtccgaaggg atctgtattc gtctctattc cgaagacgat ttcctctcgc gcccggagtt
     3301 taccgatccg gagattctgc gtaccaacct ggcctcggtt attttgcaga tgaccgcgct
     3361 ggggctgggc gatatcgctg cgttcccgtt tgtcgaagca ccggataaac gcaatatcca
     3421 ggatggcgtg cgtctgctcg aagagctggg cgcgatcacc actgatgaac aggccagcgc
     3481 ctataaactg acgccgctcg gtcgccagct ctcgcagttg cctgtcgacc cacgtctggc
     3541 gcgtatggtg ctggaagcgc aaaaacatgg ctgcgtgcgt gaggcgatga ttatcacgtc
     3601 cgcgctctcc attcaggatc cgcgcgaacg tccgatggac aaacagcagg catcggacga
     3661 aaaacatcgt cgcttccacg acaaagaatc cgacttcctc gcgtttgtaa atctgtggaa
     3721 ttatcttggc gagcagcaaa aggcgctctc ttcaaacgcc ttccgtcgcc tgtgccgcac
     3781 cgattatctc aactatctgc gcgtgcgcga atggcaggat atctacaccc agttgcgtca
     3841 ggtggtgaaa gaacttggca ttccggttaa cagcgaaccg gcggagtatc gcgaaattca
     3901 cattgcgttg ctgacgggtt tgctttccca tatcggcatg aaagatgccg ataaacaaga
     3961 atataccggc gcacgtaacg cgcgtttctc catcttcccc ggttctggtt tattcaaaaa
     4021 accgccgaaa tgggtaatgg tggcggaact ggtagaaacc agccgcctgt gggggcgcat
     4081 tgctgcgcgt atcgacccgg aatgggtgga gcctgtcgct cagcatttga ttaaacgcac
     4141 ctacagcgaa ccgcactggg aacgggcgca gggcgcggtg atggcaaccg aaaaagtcac
     4201 cgtttatggg ttgccgattg ttgccgcgcg caaggtcaac tacagccaga tcgatccggc
     4261 gttatgtcgt gaactcttta ttcgccacgc gctggtggaa ggtgactggc agacgcgtca
     4321 cgcattcttc cgtgaaaacc tgaaactacg ggccgaagtg gaagagctgg aacacaaatc
     4381 acgtcgccgc gatattctgg ttgatgacga aacgctgttt gagttctacg accagcgcat
     4441 cagccacgat gtaatctccg ctcgtcactt cgacagctgg tggaaaaaag tcagccgcga
     4501 aacgcctgat ttgctcaact ttgaaaagag catgttgatc aaagaagggg cggaaaagat
     4561 cagcaagctg gattacccga acttctggca tcagggcaat ctcaagctgc gtttgagcta
     4621 tcagtttgag cccggcgcgg atgctgacgg tgtgaccgta catattccgc tgccgttact
     4681 taaccaggtt gaggaaagcg ggtttgaatg gcagatcccc ggtctgcgcc gcgaactggt
     4741 gattgctctg attaaatcgt tgccgaaacc ggtacgccgt aattttgtac ccgcgccaaa
     4801 ctacgccgaa gcgtttttag gccgcgtcac accgctggag ttaccgttgc ttgacagcct
     4861 tgagcgcgag ttacggcgga tgaccggcgt tactgttgac cgcgaagact ggcactggga
     4921 tcaggtgccc gatcacctga aaataacctt ccgcgtggtg gacgacaaaa acaagaagct
     4981 aaaagaaggg cgctcgctac aagatctgaa agatgcgctg aaaggcaaag tgcaggaaac
     5041 gctgtctgcg gtggcggatg acggtatcga gcagagcggc ttacatatct ggagttttgg
     5101 tcagctgccg gaaagctacg aacagaagcg tggcaactac aaagtgaagg cgtggccggc
     5161 gctggtggat gagcgcgaca gtgtggcgat caaactgttt gataacccgc tggagcaaaa
     5221 acaggcaatg tggaacggtc ttcgccgtct actgctgctg aatattccct ctccgatcaa
     5281 gtatttacat gagaagttac caaacaaagc caagctgggg ctgtacttta acccgtatgg
     5341 caaagtgctg gagctgatcg acgactgtat ctcctgcggt gtggatcaac tgatcgacgc
     5401 caatggtggc ccggtctgga cggaagaagg ctttgctgcg ctgcatgaaa aagtgcgcgc
     5461 cgaactgaat gacacggtgg tggatattgc gaagcaggtc gagcaaatcc tcactgcggt
     5521 gttcaatatc aacaaacgtc tgaaagggcg ggtggatatg accatggcac tggggctttc
     5581 tgacattaaa gcgcagatgg gcgggttggt atatcgcggt tttgtcactg gtaacggctt
     5641 caaacggctg ggcgacacgc tgcgatattt gcaggcgatt gaaaaacgac tggaaaaact
     5701 ggcggttgat ccacatcgcg accgtgctca gatgctgaaa gtcgaaaacg tccagcaggc
     5761 gtggcagcaa tggatcaaca aactgccgcc tgcacgtcgt gaggatgaag acgtgaaaga
     5821 gatccgttgg atgatagaag agctgcgcgt tagttacttc gctcaacaac ttggtacgcc
     5881 ttatccgatt tcagataagc gtattttgca ggcgatggag cagattagcg gttaaccctg
     5941 ctatttgcct gataaatcta aaacccggta agcatttagc gccgggtttt ttattaattc
     6001 taaaacggta aggacaaaaa ttcaggaatt cagaaaaata caattctcag ctgcaagatg
     6061 aataatgttt atctacagca tttccttaaa agatatgtca ggcttgcgga gtggcggtta
     6121 aggaaatacg acttcctcct ttcagagtgc tccgcttctc actattatct cacgcagtat
     6181 tcttaaggga acgataagga ggaaccatga acattacccc gtttccgacg ctttcgacgg
     6241 caactataga tgccataaat gttatcggac agtggctggc gcaggatgat ttctccggtg
     6301 aggtgccgta tcaggctgat tgcgtcatac ttgcaggaaa tgcggttatg ccgactatcg
     6361 atgcggcatg taagattgct cgcgatcagc aaattccttt actgattagt ggtggtatcg
     6421 gtcactcgac aacttttttg tatagcgcca tcgcacagca tccgcactac aacactatcc
     6481 gcaccgctgg cagagcagaa gcgaccatcc tggcggatat cgctcatcag ttctggcaca
     6541 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
     6601 ttagcatcgc gctattgaat caggccgtag aacgagttca tacggctatc gttgttcagg
     6661 accccaccat gcagcggcgc acgatggcga cgttccgccg tatgactggg gacaatcccg
     6721 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
     6781 gtgtaatctt tgttaacccg ttgcaaggat tatggccggt tgagcgttat ctatcactac
     6841 tcactggcga gctgccgcgt ttacgcgatg atagcgatgg ctacggtccc cgcgggcgag
     6901 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
     6961 acgatgcggt gcttatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
     7021 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
     7081 tttacacttg tttttatgaa gcccttcaca gaattgtcct ttcacgatgc cgtctctctg
     7141 atgattgatg ctaattaaca atacatttac caaaagcaaa catataaatc acaggagtcg
     7201 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
     7261 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgtataccc
     7321 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaacctgaa
     7381 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgc gcaaaatctc cgccgggatc
     7441 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     7501 ctggctgaag tcgaagtggc ttttactgcg gactatatcg attacatggc ggagtgggca
     7561 cggcgttacg agggcgagat tattcaaagc gatcgtccag gagaaaatat tcttctgttt
     7621 aaacgtgcac ttggcgtgac taccggtatt ctgccgtgga acttcccgtt cttcctcatt
     7681 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     7741 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     7801 ggcgtgttta accttgttct ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     7861 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     7921 actgcggcga aaaacatcac taaagtgtgt ctggaattgg gtggtaaagc accagctatc
     7981 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     8041 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     8101 cagttcgtca atcggctggg tgaagcgatg caggcggttc aatttggtaa ccccgctgaa
     8161 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggtcgagcaa
     8221 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcagtagac
     8281 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     8341 atgcatgagg aaacctttgg cccggttctg ccggtggtcg catttgacac gctggaagat
     8401 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     8461 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     8521 gaaaacttcg aaactatgca aggcttccac gccggatggc gtaaatccgg tattggcggc
     8581 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     8641 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     8701 ttttcgaggg tgcgaataag ctgtgtgacg aagccatatt cgttatcgta ccaggcgacc
     8761 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacacc
     8821 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     8881 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     8941 tttccgagaa tcgataccag ttcagtgacc gaacctgttt tcaccggcac gcgttgcgca
     9001 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
     9061 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc
     9121 gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat cgtgccgact
     9181 tctatcccga aactgtcatg caaggctttg gccatcggcg caagacagtt agtggtgcat
     9241 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattgac gttataaacg
     9301 atagttttca tttcaccggc aggggcggaa atcaacacct tcttcgcacc agcatcaaga
     9361 tgcgcctgcg atttctcggc ggaggtataa aagccagtac attcgacaat gatttctgca
     9421 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
     9481 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaatgg tccgtagttt
     9541 gaatcatgtt tcagcaggta ggcgagaatt tttggggaag tgagatcatt aatagcgaca
     9601 acgtctatgt tgcttttgac ttcaagtaat cgacgcaaca ccagtcgacc gatacgacca
     9661 aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
     9721 tgatcctttc caggcttaga ccaggatggc gggatgggca atctccattc tcacagtgaa
     9781 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
     9841 gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatatt caaggttaca
     9901 aatcagcatt cactggctgg tctttttact ggttatcgca gcgtattgcg caatggagtt
     9961 tcgtggtttc ttcccacgta gcgatcggcc actcatcaac atgattcatg tttcctgtgg
    10021 catctcaatt ctcgtgctga tggtcgttcg tcttttgtta aggctgaaat acccaacccc
    10081 gccgattata cctaagccaa aaccgatgat gacggggctg gcgcatttgg gacatttggt
    10141 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
    10201 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
    10261 ggtagatagc ttaaagtcgt ggcatgaaac gctggcgaat ctgggatatt ttgtcatcgg
    10321 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
    10381 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
    10441 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
    10501 tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt aatcaattca
    10561 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgaa
    10621 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
    10681 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
    10741 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat
    10801 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
    10861 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
    10921 cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga
    10981 gtgtgaatgt cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgtgct
    11041 tcatagcctt tctccttgcc ggatggcggg taagaggcta agatctgaat tgctaggttc
    11101 attcgttggc ctcggttgat agaaatatcg gtcggggcct tcgtctttct gattcccggt
    11161 tagcctgaaa acagaaagtc tcaggcaccc gcaggcatcc tatgaggttt ccttagggac
    11221 gaaaataatc acttcacgaa attgcgtgct gttttccaga atttttcgtc attcgggtta
    11281 gccagtttag ccattcgtta ctctcttcat tccaatagca ttaattttct atgcaataat
    11341 tgttgtaaaa atgtgacgca aagaggtttt tggtcataag taattaccgt caagtgccga
    11401 tgactttcta tcaggagtaa acctggacga gagacaacgg taatgaatac aactccctca
    11461 cagcgattag gttttttgca tcacatcagg ctggttccgt tatttgcctg cattcttggc
    11521 ggtatcttag ttctattcgc attaagttct gctctggctg gctatttcct ctggcaggcc
    11581 gatcgcgatc agcgtgatgt tactgcggag attgagattc ggaccgggtt agcgaacagt
    11641 tcagattttt tgcgttcagc ccggatcaat atgattcagg ccggggctgc gagtcgtatt
    11701 gcggaaatgg aagcaatgaa gcgaaatatt gcgcaagccg aatcggagat taaacagtcg
    11761 cagcaagggt atcgtgctta tcagaatcgg ccggtgaaaa cacctgcgga tgaagccctc
    11821 gacactgaat taaatcaacg ctttcaggct tatatcacgg gtatgcaacc tatgttgaaa
    11881 tatgccaaaa atggcatgtt tgaagcgatt atcaatcatg aaagtgagca gatccgaccg
    11941 ctggataatg cttataccga tattttgaac aaagccgtta agatacgtag caccagagcc
    12001 aaccaactgg cggaactggc ccatcagcgc acccgcctgg gtgggatgtt catgattggc
    12061 gcgtttgtgc ttgccctggt catgacgctg ataacattta tggtgctacg tcggatcgtc
    12121 attcgtccac tgcaacatgc cgcacaacgg attgaaaaaa tcgccagtgg cgatctgacg
    12181 atgaatgatg aaccggcggg tcgtaatgaa atcggtcgct taagtcgtca tttacagcaa
    12241 atgcagcatt cactggggat gacagtaggg actgttcgac agggtgcgga agagatttat
    12301 cgtggcacca gcgaaatttc agctggcaat gcggacctgt catctcgcac cgaagaacaa
    12361 gcggcggcta tcgaacaaac tgccgccagc atggagcaac tcactgcgac ggtgaaacag
    12421 aatgcggata acgcgcatta tgccagcaaa ctggcgcaag aggcttctat taaagccagc
    12481 gatggcgggc agacggtttc cggtgtagta aaaacgatgg gcgctatctc cacgagttcg
    12541 aagaaaattt ctgagatcac cgccgtcatc aacagtattg ctttccagac gaatattctg
    12601 gcactgaatg ctgccgttga agccgcgcga gcgggtgagc aaggacgtgg atttgccgtt
    12661 gtcgccagcg aagtacggac actcgcaagc cgcagcgctc aggcggcgaa agagattgaa
    12721 ggcttgatca gtgaatcagt caggttaatt gacctggggt cggatgaggt ggcaacggcc
    12781 gggaaaacca tgagcactat tgttgatgcc gtcgcgagcg tcacacatat catgcaggaa
    12841 atcgccgccg cctcggatga acaaagtaga ggcataacgc aggttagcca ggcgatttct
    12901 gaaatggata aggtgacgca acagaatgct tctctggtag aagaggcctc agcggcggcg
    12961 gtgtcccttg aagaacaggc ggcacgatta actgaggcgg tggatgtatt ccgtctgcac
    13021 aaacattctg tgtcggcaga acctcgcgga gcgggtgaac cagttagttt cgctacggtg
    13081 tgaaaatgtt caaggaggga tcgacagatc ccttcacctt tcagaacggc attgattttc
    13141 gaatagcgtt aatcatcaac tggcaaccag aagagaacgt cgcatctacg cgggtcagta
    13201 ttccaatcgg ttcgcctgca ccatgtcccg gaacaggcag ggccaccagc gtggcatgac
    13261 gcaggtcgtc ttttaccgcg ccagaaggga caaaccacac gtagtcgtat tcaaccgtaa
    13321 gttgacgaga tagcgaagca gacagcgttt cgatacaacc cgaaggaatt ttacatccct
    13381 ggctctgtac taatgcatct gaatgctggc gtggcgcagt gccttctggt gatacaacga
    13441 ccggccattc cagcactcgg cttagcgtta cgttctcctg aagtagcggg tgattagggc
    13501 ggacaaccag cttcagcgat tcaagaaaca gcagttcgta attaagcccg gtcatcagtt
    13561 caggatctga catccgacca atgccgatat cgatttcccc ggttttcaaa cccgccagaa
    13621 tcatagggtt actcattgtc gcaacttgca aggtcgtttc tttttgttgc tgatgaaact
    13681 gacctataac cgaaggtaat atccccagtg ccgcagtagg tagtgcacca accctgacga
    13741 catcattatt aagaccttct ttacgatgaa gcgactgtcc ggcagtgttg atggcgtcaa
    13801 gaactctgac tgcatgcgtt aaaaattgtt cgccgggtaa ggtaagttgc gccccctgac
    13861 gaccacgctc aaacaagcga gcgcccgtca gctgctccag ttcattcaat gtcttagaga
    13921 gcgcaggttg actcaaatta agggtttcag ccgcgcgccc caaagttcct tgttgtgcga
    13981 cagctacgaa tgtatgaagg tggcgcaaac ggatgcgctg actaaacaga ctattttttt
    14041 ccataagcga tgttaaaaac gaagcggtgt cgctgacaag tgaagttgtt tgattatgat
    14101 aacttgattg caaaatatta ttaacaatta aagcaattat gttacagcaa aatagataat
    14161 attgatgttt tcgcggcgag atcacagttt gtaaattctt cccgccagag tgaatgcggt
    14221 tacctacact ccagattact gaccactgga ggcagacaat atggcgaaca gcatcacggc
    14281 ggatgagatt cgggaacagt tttcgcaggc aatgtcagcc atgtaccagc aagaagttcc
    14341 gcagtacggc acgctgctgg aactggtagc tgatgtgaat ctggctgtgc tggaaaacaa
    14401 tcctcaactg cacgaaaaaa tggtaaatgc agacgagctg gcgcgactga atgttgaacg
    14461 tcatggggcg attcgcgttg ggactgcaca agagcttgct actcttcggc ggatgtttgc
    14521 cattatgggg atgtacccgg tgagctatta cgatctctcg caggcggggg tgccggtaca
    14581 ttcgacagca tttcggccca ttgatgatgc ttctctggcg cgtaatccct tccgcgtttt
    14641 tacctcgtta ctccgccttg agcttatcga gaacgaaatt ttgcgccaga aagcggcgga
    14701 gattctacgt cagcgcgata tcttcacccc acgttgtcga ctactgttag aggaatatga
    14761 gcagcggggc ggttttaacg aaacacaggc acaggagttt gtgcaggaag ccctggaaac
    14821 gtttcgctgg caccagtcag caacggtaga tgaagaaacc tatcgcgcct tgcacaacga
    14881 acatcggttg attgctgatg tggtctgttt tcctggatgc catatcaacc atctgacgcc
    14941 acgtacgctg gatattgacc gggtgcagtc gatgatgcct gaatgcggaa ttgaaccaaa
    15001 aattctgatc gaagggccgc cgcgccgcga ggtaccgatt ttactacgcc agaccagctt
    15061 taaagcactg gaagagacgg tgttgtttgc ggggcagaaa cagggcacgc atactgcgcg
    15121 ctttggtgaa attgagcagc gtggcgtggc attaacgccg aaagggcgac aactgtatga
    15181 tgatcttctg cgtaacgctg gaaccgggca ggataatctc actcaccaaa tgcatttaca
    15241 ggaaaccttc cgcacttttc ctgacagtga gtttttaatg cgtcagcaag ggctggcatg
    15301 gttccggtac cgtctgacgc cttcgggtga ggcgcatcgt caggcgattc atcccggaga
    15361 cgatccacag cccttaattg aacgtggttg ggtcgcggcg caacccatta cctatgaaga
    15421 tttcttgccc gttagcgcgg cggggatctt ccagtcaaat ctgggtaatg aaacgcaggc
    15481 acgcaatcac ggtaatgcca gtcgcgaagc atttgagcag gcgttgggct gcccggtttt
    15541 ggatgagttc cagctttatc aggaagcgga agaacgcagt aaacgtcgct gtggtttgct
    15601 ttaaattctg accatccgcc tttgcaaaaa tttgcctgat tttacaaacg aatcaggctc
    15661 atcccatcga cataaaaaaa ttgccgattt atgcatattc tctcagttca acaattggat
    15721 tattaataaa tattgtctag agtgagcggt cataaataag cactttcttg ccgctgaaaa
    15781 cgaccagcgc gggaccattc acaacaccag aaggactcac tttcaggtat ggatcgtaga
    15841 cgatttatta aaggttcaat ggctatggcc gccgtgtgcg gtaccagcgg cattgcttct
    15901 cttttttctc aggcggcatt cgcggcagat tctgatattg ccgacgggca aacccagcgt
    15961 tttgacttct ccattctaca gtcaatggcg cacgacttag cgcaaacagc gtggcgtggt
    16021 gcgcctcgtc cgttacctga cacgctggcg acaatgacgc cgcaggctta taacagtatt
    16081 caatacgacg ccgaaaaatc gctctggcat aacgttgaga accgtcaact ggacgctcag
    16141 ttcttccata tgggaatggg attccgtcgc cgcgttcgta tgttttctgt agatccagca
    16201 acacatctgg cgcgtgaaat tcactttcgc ccggagttgt tcaaatacaa cgatgcgggt
    16261 gttgatacaa aacaattaga agggcaaagc gatctcggct ttgccggttt tcgcgtgttt
    16321 aaagcccccg aactggcgcg ccgtgatgta gtatcatttc tcggcgcgag ttatttccgc
    16381 gccgttgatg atacatatca atacggtttg tcggcccgcg gcctggcgat cgacacttac
    16441 accgacagta aagaagagtt ccccgacttt accgccttct ggtttgatac ggtaaaaccg
    16501 ggggcaacta cctttaccgt ttatgcgttg ctcgatagcg ccagcattac tggtgcctat
    16561 aagttcacta tccattgtga gaaaagtcag gtgattatgg atgtggaaaa tcacctgtat
    16621 gcgcgcaaag acattaaaca gctgggcatt gcgccgatga ccagtatgtt cagctgcggt
    16681 actaatgaac gtcggatgtg cgacaccatt catccgcaaa ttcatgactc tgatcgtctg
    16741 tccatgtggc ggggcaacgg cgagtggatt tgccgtccgc tgaataatcc gcaaaaattg
    16801 cagttcaatg cttacaccga caacaatccg aaagggtttg gtttattgca actggatcgt
    16861 gacttctccc attaccagga cattatgggc tggtataaca aacgcccaag tctgtgggtg
    16921 gaaccgcgta acaagtgggg taagggcacc atcggcctga tggaaatccc aacaacgggc
    16981 gaaacgctgg ataacattgt ctgcttctgg cagccagaaa aagctgtaaa agcgggtgat
    17041 gagtttgcat tccagtatcg tctgtactgg agtgcgcaac cgcctgttca ttgcccatta
    17101 gcgcgcgtta tggcgacgcg taccggcatg ggcggtttcc cggaaggttg ggcgccaggt
    17161 gaacactatc ccgaaaaatg ggcgcgtcgt tttgccgtcg atttcgttgg tggtgatctg
    17221 aaagctgccg caccaaaagg cattgagccg gtgattacgc tttccagtgg ggaagcgaag
    17281 caaatcgaaa ttctctatat tgaacccatt gatggttatc gtattcagtt tgactggtat
    17341 ccgacgtcgg actccactga tccggtcgat atgcggatgt atctgcgttg tcagggggac
    17401 gctatcagtg aaacatggct gtatcagtat ttcccgccag cgccggataa acgtcagtat
    17461 gttgacgacc gcgtgatgag ttaattgttt tttcttcggc accttcttcg ggaggtgccg
    17521 tctggttaaa cacgatcccg ctcgcatttt tccctaagtt aaatgagtaa tctgatggtg
    17581 tgtatttcag atacaccttg tcagccacta acagggagtg cgtatgtttc cagaataccg
    17641 agatttaata tcccgtctga aaaacgaaaa tcctcgcttt atgtccttgt tcgataaaca
    17701 caataaactt gatcatgaaa ttgccagaaa ggaaggttcc gacggtcgag ggtacaatgc
    17761 ggaagtggtc cgcatgaaaa aacaaaagct acagttaaaa gatgagatgc tcaaaatcct
    17821 gcagcaggag agcgtcaaag aggtgtaaac tttcctaagc cgccagccag gcggcttttt
    17881 taacaacagc atggattgac tggagataag atgactgaaa cgataaaagt aagcgaatca
    17941 cttgaattac atgctgttgc ggaaagtcac gtcacacctc tttatcagtt aatctgtaaa
    18001 aataaaacct ggttacagca gtcgctaaac tggccgcagt tcgttcagag tgaagaggac
    18061 acgcgaaaaa cggtgcaggg taatgtgatg ttgcatcaac gcggctatgc caaaatgttc
    18121 atgattttca aagaagatga acttatcggc gttatctcgt ttaatcgtat tgaaccactg
    18181 aataaaactg ctgaaatcgg ctactggctg gacgaatctc atcaggggca ggggatcatt
    18241 tctcaggcgc tgcaggcatt gattcatcat tacgctcagt ctggtgaact tagacgcttc
    18301 gtgatcaaat gtcgggtgga caatccgcaa agcaaccagg tcgctttgcg caatggtttt
    18361 atccttgaag gttgcctgaa acaggctgag ttcctgaatg atgcctatga tgatgtgaac
    18421 ttatacgcgc gtattatcga ttcataataa ccctgccagc ggcgttcgcg taatgcgctc
    18481 ctcgacgtta atgacttttg gaccacggac atgaaccgtg tcgccatcaa aggcttcaac
    18541 gacggcgtgg tcggttaact ccacatggtt ttcaattatc accgcacctg taatacggct
    18601 ctcaccttgt attacaacgt gctcgtcgag cagaatcggt ccgccacgta ccacggcatt
    18661 tccgccaatc aagacatgat gttttaacac acaattacct ttcacaatgg catattccgc
    18721 cacctgcgaa ctgtaatgaa tcgtcggaat ggcatcttct tctatgccag ctttcacctg
    18781 cgcgtgaccg tagactttag cgcaatcgca tagccatacg ttgttctctt cattaccttc
    18841 aatactggca aaatcaaaaa cttcggcgcg atgttcaata aaagcatacc ggacgacggc
    18901 atcgccataa atttgtgcct ggtggacaat acgcgaggcg ctaacccttg cgcgatcata
    18961 aatttgtagc aggagttgat ggtcgggcgt taagccttgt gcggcgacga tcatagaatg
    19021 ttgatcaatt aaggcgtgac caaatattct acattgcccg taaactaatg agtcatgaat
    19081 ggtgacactg tcacttatat atgcaccctg gctgatttca ctattatcga tccagacgtt
    19141 atccgttgcg taaacctctc cccacagcac gctggtgccg gtgatacgcg tattaccgga
    19201 aatcaccgtt ccgccgaacg caatagcatt ctggtcgtaa atccagcaat ttccttcctg
    19261 tgctaacacc gtttcgcgat cgatccagcc gccagcggtt cctgctatca catcgttaaa
    19321 atcgctaatg gcgataatct gccgtaataa cacacttttt ttagtgccat cttcctgata
    19381 actaaaggct cgctgttctt cacttagacg atatttgcgc atagcgtttt cccacaggtg
    19441 acttactata accgtagcaa attctgcggc tctggctatg ctcgagaaaa ttccataaaa
    19501 tgcatttcaa atatacttta taaattaaac
//
LOCUS       DAOFQD010000087        19433 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.087, whole genome shotgun sequence.
ACCESSION   DAOFQD010000087 DAOFQD010000000
VERSION     DAOFQD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.087"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            108..1199
                     /gene="rutA"
                     /locus_tag="QBF46_003196"
     CDS             108..1199
                     /gene="rutA"
                     /locus_tag="QBF46_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDQ6675346.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            1199..1891
                     /gene="rutB"
                     /locus_tag="QBF46_003197"
     CDS             1199..1891
                     /gene="rutB"
                     /locus_tag="QBF46_003197"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDQ6675347.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFAHIA"
     gene            1903..2289
                     /gene="rutC"
                     /locus_tag="QBF46_003198"
     CDS             1903..2289
                     /gene="rutC"
                     /locus_tag="QBF46_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDQ6675348.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            2297..3097
                     /gene="rutD"
                     /locus_tag="QBF46_003199"
     CDS             2297..3097
                     /gene="rutD"
                     /locus_tag="QBF46_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDQ6675349.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQVLVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            3107..3697
                     /locus_tag="QBF46_003200"
     CDS             3107..3697
                     /locus_tag="QBF46_003200"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDQ6675350.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            3708..4202
                     /gene="rutF"
                     /locus_tag="QBF46_003201"
     CDS             3708..4202
                     /gene="rutF"
                     /locus_tag="QBF46_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDQ6675351.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            4223..5551
                     /gene="rutG"
                     /locus_tag="QBF46_003202"
     CDS             4223..5551
                     /gene="rutG"
                     /locus_tag="QBF46_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDQ6675352.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(5809..5982)
                     /locus_tag="QBF46_003203"
     CDS             complement(5809..5982)
                     /locus_tag="QBF46_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDQ6675353.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            6967..7515
                     /locus_tag="QBF46_003204"
     CDS             6967..7515
                     /locus_tag="QBF46_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6675354.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGTTKA
                     TSRDGFRDMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVESLQSKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSETVKTLISQG
                     KTRLQIQEELGISRATYYRLAK"
     gene            complement(8992..9180)
                     /locus_tag="QBF46_003205"
     CDS             complement(8992..9180)
                     /locus_tag="QBF46_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675355.1"
                     /translation="MVNLPPELGSPDVWAGGGVGLIAGLLLRYFIVRVFIVFRLFGRE
                     WSFDLKDRGREDNHAEPR"
     gene            9478..10029
                     /locus_tag="QBF46_003206"
     CDS             9478..10029
                     /locus_tag="QBF46_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675356.1"
                     /translation="MNDREFQRFLEESKARNRHNGYSYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRYMSVRKEKENTLKTFLMGFDSYEQLPAKIEDLIIGTCRSFGRDNYH
                     RKIFYLLRNIDKISSSTITSYLQRQATRLSHELPSDKYCANLATICMKVIETINHHVE
                     VGNISLTTSEPDFEFDPYILEEF"
     gene            10031..10309
                     /locus_tag="QBF46_003207"
     CDS             10031..10309
                     /locus_tag="QBF46_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675357.1"
                     /translation="MNKHTLTRISEEAFHELTRIKQTTNLPHQTVMQLAIAKEVTESD
                     LLKYPVSKGRKHIRISQDALATLKALNGFKIDIARLLSIKIHKLSMGV"
     gene            10309..10524
                     /locus_tag="QBF46_003208"
     CDS             10309..10524
                     /locus_tag="QBF46_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675358.1"
                     /translation="MLCITFEYHTDKMIRYISDLLIKGNGFGDIHNSKDIFIKAIGPN
                     ETLKTAVKPEWFERHKIELGYWGEEVL"
     gene            10521..10808
                     /locus_tag="QBF46_003209"
     CDS             10521..10808
                     /locus_tag="QBF46_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ6675359.1"
                     /translation="MKPWHEDVRRYFTEHLIYDESSDSLCWSDGESVTINTDDYGNKT
                     FNIGRYTFYVKYVVWFLYHGYQSNKQIIHRNGNRADTRPKNLMQVRDFKRN"
     gene            10965..11288
                     /locus_tag="QBF46_003210"
     CDS             10965..11288
                     /locus_tag="QBF46_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675360.1"
                     /translation="MNNYELQIFIDTDTAMMIQAFTDVGVSIDFDRLLRLMADNSETI
                     EDFIQSVEFNEPRMMLPIKDSNMKRLVIEQTNKYSISPERYLKAAIAILYADNILVTD
                     SKVVH"
     gene            11817..12527
                     /locus_tag="QBF46_003211"
     CDS             11817..12527
                     /locus_tag="QBF46_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6675361.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQTEHPAITIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLKLDIAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFVYYEGNFNDGFGTPVEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDSMKAIISDYETQKKIAM
                     QADIEQLVKISTKYAKVL"
     gene            12549..12758
                     /locus_tag="QBF46_003212"
     CDS             12549..12758
                     /locus_tag="QBF46_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675362.1"
                     /translation="MFEERLETIRSVCENLKLQNKPTLRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            12865..15045
                     /locus_tag="QBF46_003213"
     CDS             12865..15045
                     /locus_tag="QBF46_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDQ6675363.1"
                     /translation="MAQFKVDSFIVELGFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRLKNTRAFKIKTEIEDTLKPLKQPRQ
                     PRISGNRAITAAYSANMSKLKGFDPILQKYIKSQFYGLSAKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTTSINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAETFIRGLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILKAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKILSSDTLPKVAKQMGLMIDKAGGWAEVAKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVTTGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANIHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWGRIFNSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQSLRDEISALSKRIQE
                     PVKVSLGGEVAIKPDETSFMTFSSNIYDQYAEATLLSSSFPEDD"
     gene            15202..15990
                     /locus_tag="QBF46_003214"
     CDS             15202..15990
                     /locus_tag="QBF46_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675364.1"
                     /translation="MIFSNQIRYSGKDGIYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGYEGSLLLTRQGQLVENFIDTLESWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     KTATTNDGGVTSKKNAGNVTTERNNAPMLSCKELMDISDVSGYAPAIRNDILDAQIKC
                     NKAWKLDYSDLTEAEKTNVTNANSNAGRVASKLKKYSVNPNKRY"
     gene            complement(15987..17630)
                     /locus_tag="QBF46_003215"
     CDS             complement(15987..17630)
                     /locus_tag="QBF46_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6675365.1"
                     /translation="MKLLVTYIRWSTREQDSGDSLRRQTNLIDAFYSKHKEEYYLLPA
                     HKYIDKGKSGFHQQHKNQGSDFRRMFDNVMSGTIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVKLIKHIIIAERAHQESLVKQKRIAQVWN
                     HKTQLARELKKPMGKQAPGWLELSEDGSHYIVDEDKASLVNIIYDKRLSGMSMFAICK
                     WLNEQGYPTINQRKVRISKTKKPDGNWSALSVKHILTSRSVLGYLPAKISSEDRKTVL
                     REEIESFYPQIVTDSKFYAVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTP
                     TGIRKPVYQGTYRCNGNKESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATETAKLD
                     ELQRRLNTVDSELEKLTETLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVS
                     SLDLSGLDMESVEGRTEAQIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDD
                     HTLTLEEATDEMQSLDDMLIFGEPVTRIYPAGDMEEVDA"
     gene            17682..18287
                     /gene="wrbA"
                     /locus_tag="QBF46_003216"
     CDS             17682..18287
                     /gene="wrbA"
                     /locus_tag="QBF46_003216"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDQ6675366.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            18308..18535
                     /locus_tag="QBF46_003217"
     CDS             18308..18535
                     /locus_tag="QBF46_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDQ6675367.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(18573..>19433)
                     /gene="agp"
                     /locus_tag="QBF46_003218"
     CDS             complement(18573..>19433)
                     /gene="agp"
                     /locus_tag="QBF46_003218"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDQ6675368.1"
                     /translation="AFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAFSEKAVAAMEKE
                     LSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQEPGVSGPLKVGN
                     SLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTSPEVARNVAKPL
                     VSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNERTPIGGKIVFQ
                     RWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCPIDANGFCPMDK
                     FDSVLNEAVK"
BASE COUNT         5384 a         4251 c         4585 g         5213 t
ORIGIN      
        1 aaaaactggc atccgctttg caaataagcc aatacgcggc tgaaaaggat atgcaggatg
       61 cagcgccccg tctgactttc actttacgag acgaagagag gttgatgatg aaaattggcg
      121 tattcgtacc tattggcaac aacggctggc tcatttcgac ccacgcgccg cagtacatgc
      181 cgacctttga actgaataaa gccattgtgc aaaaagcgga gcattaccat ttcgatttcg
      241 ccctgtcgat gatcaaactg cgtggctttg gcggcaaaac tgagttctgg gatcacaacc
      301 ttgagtcgtt caccttgatg gcgggtctgg cagccgtgac ctcgcgcatt cagatttacg
      361 ccactgctgc caccttaacg ttacctccgg caatcgtcgc ccgtatggcc gcaaccatcg
      421 actccatctc tggcgggcgt tttggcgtca acctcgtgac tggctggcaa aagcccgagt
      481 atgagcagat gggtatctgg cctggcgatg actatttctc ccgtcgttac gactatctca
      541 ccgaatatgt tcaggtgctg cgcgacctgt ggggcacggg gaaaagcgat tttaaaggcg
      601 attttttcac catgaatgat tgtcgcgtca gtccgcaacc gagtgtcccc atgaaagtga
      661 tctgcgccgg gcaaagcgac gctggcatgg cgttctccgc tcagtatgcc gatttcaact
      721 tctgtttcgg caaaggcgta aatacaccta cggctttcgc cccgaccgct gtgcggatga
      781 aacaggccgc agagcaaacc gggcgcgacg ttggctctta tgtgttgttt atggtgattg
      841 ccgatgaaac cgacgatgcc gctcgcgcca aatgggaaca ctacaaagcg ggcgcggatg
      901 aagaggcgtt aagctggcta accgaacaaa gtcagaaaga tacccgctcc ggtactgaca
      961 ccaacgtccg tcagatggcc gatcccactt cggcggtaaa catcaatatg gggacgttag
     1021 tcggttctta cgccagtgtc gcgcgcatgt tagatgaagt cgcaagcgtg cctggtgcag
     1081 aaggcgtgct gttgaccttc gatgattttc tgtcgggaat cgaaaccttc ggcgagcgca
     1141 ttcaaccact gatgcagtgc cgcgcccatc tccctgcgct gactcaggag gtggcatgat
     1201 gacgacctta accgctcgac cggaagccat taccttcgat ccgcagcaaa gtgcgctgat
     1261 cgtggtggat atgcaaaacg cttatgccac gccaggcggc tacttagatc tcgccgggtt
     1321 tgatgtctca accactcgcc cggtcattgc caacattcaa accgccgtga ccgcagcgcg
     1381 agcggcaggg atgctgatca tctggtttca aaatggctgg gatgaacagt atgtcgaagc
     1441 tggcggaccc ggctcaccga attttcataa atcgaacgcc ctgaaaacca tgcgtaagca
     1501 gccgcagctg caggggaaat tgctggcgaa aggctcctgg gattatcaac tggtggatga
     1561 actggtgccg cagcctggcg atattgtgct gccgaagccg cgctacagcg gtttcttcaa
     1621 tacgccgctg gacagcattt tgcgcagccg tggaatacgc catctggttt tcaccggtat
     1681 cgctaccaac gtctgcgtcg aatcgacgct acgcgatggc ttttttctgg agtatttcgg
     1741 cgtggtgctg gaagacgcaa cacaccaggc ggggcctgaa tttgcacaga aagccgcgtt
     1801 gttcaatatc gaaacctttt ttggttgggt cagcgacgtc gaaacattct gcgacgcgct
     1861 ttctcccacg tcctttgctc atatcgctta aggagtttaa cgatgccaaa atccgtaatt
     1921 attcccgctg gcagcagcgc accgctggct cccttcgttc ccggcacgct ggctgatggc
     1981 gtggtgtatg tctccggtac gctggctttt gatcaacata ataacgtgct gtttgccgat
     2041 gacccaaagg cgcaaacccg ccacgttctg gaaaccatcc gcaaggtgat cgagacggcg
     2101 ggtggcacga tggcggatgt gaccttcaac agcatcttta ttaccgactg gaaaaattac
     2161 gccgcgatta acgaaatcta cgccgagttt tttccgggtg ataaaccggc gcgattctgc
     2221 attcagtgcg gactggtaaa acctgacgcg ctggtggaaa tcgccacaat tgcgcatatc
     2281 gccaagtgag gccgcgatga aactttcact ctcacctccc ccttatgctg atgcacccgt
     2341 agtggtgttg atttcgggtc ttggcggtag cggcagttac tggttaccgc aactggcggt
     2401 gctggagcag gagtatcagg tagtctgtta cgaccagcgc ggcaccggca ataatcccga
     2461 cacactggca gaagattaca gtatcgccca gatggcagcg gaactgcatc aggtgctggt
     2521 agccgcaggg attgagcatt acgcggtggt cggccatgcg ctcggtgcgc tggtgggaat
     2581 gcagctggcg ctggattatc ccgcgtcggt aactgtgctg gtcagcgtta acggctggct
     2641 acgaataaac gcccatacgc gccgctgttt tcaggttcgc gaacgattac tgtatagcgg
     2701 tggcgcgcag gcatgggtgg aagcgcagcc gttgttcctc tatcccgccg actggatggc
     2761 ggcccgcgca cctcgccttg aggcagaaga cgcgctggca ctggcgcatt ttcagggcaa
     2821 aaataattta ctgcgtcgac ttaacgccct caaacgcgct gactttagtc accatgcgga
     2881 tcgcatccgc tgcccggtgc aaatcatctg cgccagtgat gatctgctgg tgccaacagc
     2941 atgttccagt gaacttcatg ccgccctgcc cgatagccag aaaatggtga tgccctatgg
     3001 cggacacgcc tgcaacgtga ccgatcccga aacgtttaat gctctgttac tcaacgggct
     3061 tgccagcctg ttacatcacc gtgaagccgc cctgtaagga attgctatga acgaagccgt
     3121 tagcccaggt gcgcttagca ccctgttcac cgatgcccgc actcacaacg gctggcggga
     3181 gacacccgtc agcgatgaga cgttacagga gctttatgcc ctgatgaaat gggggccgac
     3241 atcagctaac tgttctccgg cacggatcgt gtttatccgc actgcagaag gaaaagaacg
     3301 tctgcgcccg gcgctttcca gcggcaatct gcaaaaaacc ctgaccgcgc ccgtcaccgc
     3361 tatcgtcgcc tgggacagtg aattttatga acggttacca ctactgtttc cccacgggga
     3421 tgcccgcagt tggtttacct ccagcccaca acttgccgaa gaaacagcgt ttcgcaacag
     3481 ttccatgcag gcggcctatc tgatcgtcgc ttgccgtgcg ctgggactgg ataccggccc
     3541 gatgtcgggt tttgaccgtc aacacgttga cgacgccttt tttgcgggca gcacgctgaa
     3601 gagcaatctg ctgattaata tcggctatgg cgatagcagc aaactttttg cgcgcctgcc
     3661 acgtctgtcc tttgaagaag cctgcgggct gttgtaagga gcaaaccatg aacattgtcg
     3721 atcaacaaac ctttcgcgat gcgatgtcct gtatgggcgc ggcagtcaat atcatcacca
     3781 cggatggtcc agccgggcgc gccgggttca ccgccagcgc ggtctgcagc gtgaccgaca
     3841 cgccgcctac attactggtg tgcctgaatc gtggggcgtc cgtctggccg gtattcaatg
     3901 aaaatcgaac gctgtgtgta aatacactta gcgccgggca ggagccgctt tcaaaccttt
     3961 ttggcggcaa aacgcccatg gaacaccgct ttgccgccgc ccgctggcag actggtgtga
     4021 ccggatgccc gcaactggaa gaggcgctgg tttcgtttga ctgccgtatc agccaggtgg
     4081 tgagcgtcgg cacccacgac attttgtttt gcgccatcga agcgattcat cgtcacgcca
     4141 caccctacgg gctggtgtgg ttcgatcgca gttatcacgc gctgatgcgc cctgcttgtt
     4201 aacctcctta aggagacagc tgatggcaat gttcggtctt cctcactggc agttaaaatc
     4261 gacctctaca gagagcggcg tggtcgcgcc ggatgaacga ttgccgtttg ctcagacggc
     4321 aataatgggc gttcaacacg cggtggcgat gtttggcgcg acggtgttaa tgccgattct
     4381 gatggggctg gatcccaatc tttccatttt aatgtcgggg atcggcacat tactgttttt
     4441 ctttattacc ggcgggcgcg tgcccagtta tctcggctcc agcgcggctt ttgtcggcgt
     4501 ggtgatcgcc gcaacgggct ttaacggtca gggcattaac ccgaatatca gcattgcgct
     4561 gggcgggatt atcgcctgtg gactggttta taccgtcatt ggcctggtgg tgatgaaaat
     4621 cggtacgcgc tggattgaac ggctaatgcc gccggtggtg acgggtgcgg tggtgatggc
     4681 gattggcctg aacttagcgc ctatcgcggt aaaaagtgta tcggcttcag gctttgatag
     4741 ctggatggcg gtaatgacgg tgctgtgtat tgggctggtg gcggtgttta cccgtggcat
     4801 gatccagcgg ttattgattc tcgtagggtt aattgtcgcc tgtctgctgt atggcgtgat
     4861 gaccaacgtt ttagggctgg gaaaagccgt cgattttact ctcgtcagcc atgctgcctg
     4921 gttcggtttg ccacattttt ccactcctgc ttttaatggt caggcaatga tgctgattgc
     4981 gcccgtggcg gtgattctgg tggcggaaaa cttaggccat ctcaaggcgg tcgccgggat
     5041 gaccggacgc aatatggatc cgtatatggg acgggcattc gtcggtgatg ggctggcgac
     5101 gatgctttct ggttctgtcg gtggcagcgg cgtaaccacc tacgccgaga atatcggtgt
     5161 aatggccgtg acaaaagtct actcgacgct ggtgtttgtc gctgctgccg tcatcgccat
     5221 gctgttgggc ttttcaccga aatttggcgc gctgattcat accattccgg ccgcggtgat
     5281 tggtggtgca tcaattgttg tgttcgggct gattgccgtc gcaggcgcaa ggatatgggt
     5341 acaaaaccgt gtcgatttaa gccagaacgg taatttgatt atggtcgcag taaccttagt
     5401 gctgggcgcg ggtgattttg ctctcacgct gggcggtttt acgttgggag gaatcggtac
     5461 agcaaccttt ggcgcgattt tactcaatgc gttattaagc agaaggttgg ttgacgttcc
     5521 accgccagaa gttgttcatc aggagccata aaaagaaaac cgccgggatt acccggcggt
     5581 gcagattgtt gacaacgtac gcattgttca tgccggatgc ggcgtaaacg ccttatccgg
     5641 cctacaaaac agtgcaaatt caatatattg caatcttctg gtaggcctga taagcatcgc
     5701 gcatcaggca agcttacatt tgtcatcagt atcaaccacc gggtaaccgg cggtgatggc
     5761 tttttgacga gctacagaca aaaatgcatt cgtcactgat tagtccggct agttgtcgct
     5821 tttgccgtga ctgctcttgc cacctttttt acctgcttca gatgcgcgct gcgggtcatt
     5881 cttgaaatta cccccgctgt gctgtccacc ttttttacct gcttctgatg ctctttcgcg
     5941 gtcttctgca aaattgccgg aaccgcctcg atggtttgcc attactgacc tccgtctggg
     6001 tatagacatc atattgcata gaattaacgc ggcgtatggc ggctgccata ttttcgctac
     6061 gtgggactaa gcttagtgaa ctgacgcggg cagacccgcg tttggtaata tcctgcaaca
     6121 gcttcttagc gagaatagat atcatctcca ataattatcc ataagccgct cttatgatta
     6181 taaaaacgcg atgagataaa aatgtgtcat tttgcgacaa aattgcgtgc ttgtgaaagt
     6241 tgttataaat caaataagtg gttgtgaaat ttgcactctg aaaaggacgt cttatcttta
     6301 aataagtggt agcgaatcgc tacggaatag agataacacg aggagtggtt agaaatggct
     6361 aaagttctgg tgcttttaca tagctaaaca cgtacattca tctacctgta aaaacaggac
     6421 aatatcaggt gaatccctat gcaatttaac ggtgtttttc cgagtatcta agcagttcta
     6481 cacagattct atgcagtatc ggtatagtcc ctttttatca taacttattg aaatatataa
     6541 agaatctgtg aagtctcttt tcagggtaaa ctgtatcgtg ttagtcgtcc ctctctcgac
     6601 tcctagaaaa gtgctatata caggagcata taacgctata tagatgtttg gtcaccatac
     6661 gttacagttg caagagataa caatcagcta tgtgaaagtt aggaagtgat ggttatagtt
     6721 accctcctaa aatcttcata gttgattcgg ttagttgggc ttgttatgag gatgcttcat
     6781 ccgaatcatc taccaacccc taatcaacct catagctcct tcataaccag tcttaaaagg
     6841 acttcctttt gagacacttt caacgtaacg gtagaatgca ccctcaagta gtctcaaaag
     6901 attgtttttt gattatgaga cagctaaact tatgagacta acttttgaga cttctaaggg
     6961 ggatgtatgg cggtgattgg ttattgcagg gtatcaacac aagaccagac tgtagacaac
     7021 cagaaactac agatagagaa ggttcacaag gtcgataaat ggtttattga tgaggctgtc
     7081 tcaggaacta caaaggccac cagcagagat ggttttagag acatgatgaa ctatgtaagg
     7141 tgtgacgata cgttagttgt ggtagctatt gaccgtttag ggcgtaatac cgttgatgtg
     7201 ctctctaacg tggagtcact acagtctaaa ggtgtgagag ttgtttcttt gcgtgaaggt
     7261 ttcgacttat taacaccaat cggtagagca atgcttacta tgatggctgg tttagctgac
     7321 cttgaaaaag accttatcag tgaacgtaga aaggctggta ttgaacgtgc taaagctgaa
     7381 ggggttcaca tggggagacc cgtaaaagct tcttcagaga ctgtaaagac gcttatttca
     7441 caaggtaaaa cgaggttgca gattcaggaa gagttaggaa tctctagagc aacttattac
     7501 agacttgcta aataagttac tgaatagtta catggatgtg actgatattt ggcttttttg
     7561 caaagggggt cacatagctg gtgtgacggg tacttagcag gtatacatag ccttgtgata
     7621 tttttgaaag atttggttga agagacttag aagagacctg attatttttt ataaaaattt
     7681 tcagagtctg aaaagttact gtacagaata tatacaaact aaaaaggact cccgtaggtg
     7741 attcagtaat gaattacttc atgaagagtc cctcatagtt gattcggttt agtaaataaa
     7801 cctcatagtc gatttttaac gtggatacga caatccaccc ataccagacc caaccacacc
     7861 ctacctagcc gtaccctacc tcgccagact ataaggaggt gtagagaaac cgttaaggtg
     7921 tctgtacagt tggtgattat acatattgtg atgataacat caagaaactt ttaacaggag
     7981 gggttcccct atggtcacat atcccctctc tcgcttagtg agctacacag acctagagaa
     8041 catgctctgt aacgccttca caaacattga ctaaggtcaa cttcataaac ccagagttaa
     8101 gttaccttga aagattctac caagaaacta cacaggtgtt atttagtctg actgtgaagg
     8161 gatatttaac gggagaagag atacgatata acccgaaggg gagagtatca ccttaacagc
     8221 tactgtaaga gtaagactaa aaaactctta acagataact accaagaaga gagggaacga
     8281 taagaccaga ggtagtgacc tgttaaacag ttcgtttcaa agctcttaag agtctgttaa
     8341 gtaagggcca tgtgaacaat cataccattt tgtcaatgct tttgtctttg acctaaatca
     8401 ataaaatgta tcgtttgata tggttttaag caaagtagct ataaaacttt ttagaggggc
     8461 gcttcgcttg tgtcaccaat attattcttc cctgtctaag agggaagtta cagggacatt
     8521 tcaaagccct caataggcaa accgaggaac acaggaaacc gaggggagaa aaactattgt
     8581 gattaccttg agcaaggtct gtaaaggtcg tttaagaggt cggttaagaa aggagcttcc
     8641 ctctctgggg ttatcgctct tctcccgatt agtggggcgg ctaagagggc ttgaagttat
     8701 ctgtgaaggt actggacagt cttatatatt gggtgattac aactcgttga aaaataagca
     8761 tttttagaag ttactgttaa ggttgcagag ttgtctgtga agagacttgg cagttctcta
     8821 tatatggcta attacaactt attgaaaaat aagaactttt agaaattact gtcagagagg
     8881 tgagtgctta atagatacag ctttccggct ctgtcaagct attttttaat agatagcaca
     8941 aaatgctaaa gataccccta taccatcacg tacagggggt tctgaaggag tttaacgggg
     9001 ttctgcgtgg ttgtcttcac ggcctctgtc cttgaggtca aacgaccatt cacgaccaaa
     9061 caatctgaac acgataaaca ctctgacgat gaaatagcga agtagtaagc ctgcaatcag
     9121 cccaacacct ccaccagccc atacatcagg tgaccccagc tcaggaggga gatttaccat
     9181 tagttttcct tgtgagacgc cctcaatcgc attctgacac aaaaagtacc tgaccttatg
     9241 agttgacctg aagaggccat ttaactcgtt acagagcgat tgaagaggtc tgtgtgcaat
     9301 gcaataacca tgtgaccatt ctcatagcca cgcctcatat tttactctcc ccatagaacc
     9361 attataccac agatgttaat gaaatgttaa aaaatgcttg acattagtct ttgaaaatgc
     9421 tagtattgtc tatataagtt aacagaataa taacatctta tgagggtcag aacatgaatg
     9481 aacgacagag aatttcaaag atttcttgaa gagagcaaag caagaaacag acacaacggt
     9541 tacagctaca caaacaaccc caccagctac gaagtcccaa cctttagcaa atccgaacgt
     9601 aagaacattg aagcggtcat tcgtagcatt acgccacgag acagatatat gtcagtacgc
     9661 aaagaaaaag aaaatacttt aaaaactttc ttgatgggct ttgattctta cgagcaactg
     9721 ccagcaaaaa ttgaagattt aattatcggc acttgtcgca gctttggaag agataattat
     9781 caccggaaga ttttctattt actacgaaat attgacaaga ttagctcaag cacaataaca
     9841 agttatctac agcgtcaagc gacccgatta agccatgagc taccaagtga taaatattgc
     9901 gctaacttag ccaccatttg catgaaagtt attgagacaa ttaatcatca tgtggaagtg
     9961 ggcaatatta gccttacaac cagtgaacca gattttgaat ttgaccccta tattttggag
    10021 gaattttaaa atgaataaac acactttgac ccgtatttca gaagaagctt ttcacgagct
    10081 tacccgaatt aaacagacaa ctaacctgcc tcatcaaact gttatgcagc ttgcaattgc
    10141 taaagaggtt actgagtcgg atttactgaa atatccagtc tctaaaggtc gtaaacacat
    10201 ccgaattagt caggacgctt tagctacttt gaaggcgctt aacggcttta agattgatat
    10261 tgcccgtcta ttgagcatca agattcacaa gctatcgatg ggggtttaat gctatgcatt
    10321 acctttgaat accacacaga taaaatgatt cgctacatct ccgacctcct gattaaagga
    10381 aacggattcg gtgacatcca taactcaaaa gatattttca ttaaagccat tggcccaaat
    10441 gaaacactga aaacagcagt taaaccggaa tggtttgaac gtcataagat tgaattaggt
    10501 tattggggtg aggaggtttt atgaaacctt ggcatgaaga tgttagacga tattttacag
    10561 aacaccttat ctatgatgaa tccagtgatt ccctgtgctg gtctgatgga gagtcagtca
    10621 cgataaatac cgacgactac ggcaacaaaa catttaatat aggccgatac actttctacg
    10681 tgaaatatgt tgtctggttc ctttaccatg gctatcaatc caataagcaa attatccatc
    10741 gaaatggtaa cagagccgat accagaccaa aaaatcttat gcaagtccga gactttaaga
    10801 gaaattagaa aaatagcttc tcagacgctc tcaatcgcat tctaacgcaa aaaactatct
    10861 tattttaggg tgggtaatac cactgtacag gctacccctg aaaaagactg tttagctcgt
    10921 tacagagcga ttgaggaggt ctggtaaaaa ggagatttta ggaaatgaac aattacgaat
    10981 tacagatttt tattgatacc gacaccgcca tgatgattca ggctttcact gatgtaggtg
    11041 ttagcattga ctttgacagg ctgttaaggc taatggctga taactcagaa acaattgaag
    11101 attttattca gtcggtggag tttaacgagc ctcgaatgat gctaccaatt aaagacagca
    11161 acatgaaaag attggttatc gagcaaacga acaaatacag cattagccct gaaagatatc
    11221 tcaaggcagc tatagcaatt ttgtacgctg acaatatttt ggtgacggat tcgaaggtgg
    11281 tgcattaatg gaattggtga ttatctgttt tgtattgatt ggtatgattg caggtgcatc
    11341 tattaaagta taaatgttac caatctgtta aaaagctgtt gacaaataac cttgagcatg
    11401 acataattgt atatataggg agagcaattc tccttgaatt aaaatattaa tcgtctctac
    11461 atcgatgata actatattct gagttggaaa ggtgcaatgc ttttcgtcat ggcctttcag
    11521 gttagtctga gaggctatct caaaaagtgc ttaatcattc attcattgaa ccctttacag
    11581 ctttgcccta atcatgagtt agggcgtttc tttaagagtt ttcaaacaga acttttaaag
    11641 aaagtttttc ctgaaacttc gttaacgtta taaagcaaac ctccaaagcc cttcacagat
    11701 tatgtttagg gcttttctta atagagcatc tttaataaat cgttaagtca tcttctaagt
    11761 gtattggcgt tgaaggtgct ctattaagaa ataaaccaca cttagaggaa gttataatga
    11821 caaacgattt atatattaac ggtaaaaagg ttgatagctt cggtgactgg tcgccacaga
    11881 ctgaacatcc agctatcact attcagcgtc aagagcatga caagcgcatt gagcttgagc
    11941 aagagttaaa gatgtcacca aaggtaattt cctttgtgtc tcctgagcct caagaaatgc
    12001 ctgatatctg caaaactgaa gcactatttg agcttgagcg tgaatattac ccagctttaa
    12061 aatcacagcg tcttaaactt gatattgctt acgatgctgt taagaacttc gaagaaacct
    12121 cgaaaccttc agaatatgat atcgcctacg agattaagtc aaacccgttt gtctattatg
    12181 aaggaaactt taatgatggt tttggtacac cagtagaaga tgtcccaaaa gttcttcaaa
    12241 gtatccctga agggttccgg ttaattgatg tcagacaagc attaaggggt tctggtcaat
    12301 ttatcctgat gactgataag acagacgaag agattcttca gttagctgaa gctaatttga
    12361 ctaagcatta ttccggtaaa ctggataagt taaagcaaaa gcttaaaaaa gaacttgata
    12421 gcatgaaagc gattattagt gactatgaaa cgcagaagaa gattgctatg caggctgata
    12481 ttgagcagtt agtgaagatt tcaacgaagt acgcaaaggt gctttgatta cgaattaaga
    12541 ggtgattaat gtttgaagaa cgtttagaga ctattcggtc tgtttgtgaa aacttaaagt
    12601 tacaaaataa gccaacctta agaattaaaa acaaacgtca agttatcacc tcacataaac
    12661 caaaaacacg caagattcca aaatggtgta ttgaccgtat cccttctgat gctcaaatca
    12721 taggtgaaac ggaattacat tatcttgtca gacattaaaa aaagaattgg ttgggtaaaa
    12781 taaacgggag agattatctg gatggcaatc taggtaactc tccttttttt gtatatgaac
    12841 gcttaaagag gtgaaatatg aacaatggca cagtttaaag ttgatagttt tatcgttgag
    12901 ctaggcttca gcgagaacgt tattaagggc ttgcaaagag ttgaaaaggc agctttgcag
    12961 tcagcacaaa gaattgaacg taatttgaat agagcgttta aggtcgatac caagcaactt
    13021 gacagcaatc tgacaagttc tttagggcaa cttgaaagga agtttaataa gacctttgac
    13081 aagattgagc agcgtcttaa aaacactaga gcgtttaaga ttaaaacgga aattgaggat
    13141 actttaaagc cacttaaaca accgagacag ccacgaatta gcggcaacag agcaatcaca
    13201 gcagcttatt cagctaatat gtctaagcta aaaggctttg accctatttt gcagaaatac
    13261 atcaaatctc agttttacgg gctatctgct aaagctggta atattggaag tgaaaagttt
    13321 aacgctgaac ttgcaaaatt aaatcaatct cttcgtgaaa cgttgaataa actcaggtca
    13381 cagacaaaga ccaccagcat taacaataca agcgacgcat ttaataatct tgcaagcaat
    13441 gcgattaaat tatctggtgc attttactct gtgatgggtg ctcttaacgc ttacaaggcc
    13501 attatgaatg caggtcttaa acgagattcc gcacagagag ccgctaagtt tgtacttggt
    13561 gataaagcat ctgaggctga aacatttatc aggggactgg ctgacaagac aggtcttaat
    13621 atctctgaag ggctatcaag ctacgctaag tttgcagcag gtgctcaggg ttctatgtca
    13681 caggaacaga cacaggagct atttggtaat gcaacggcta tgagtcgctt aatggggctt
    13741 tctaacgatg agctaaacgg cattttgaaa gcatttgagc agatggcgag caagggcaag
    13801 attcaggctg aagagcttcg tggacagtta ggtgaccgta tggctggtgc atttaagcta
    13861 ttcgctgaag cattgggtat gactgctaca gagcttgatg cagccatgaa aaacgggaag
    13921 attttaagta gtgatactct ccctaaagtc gctaaacaga tgggcttgat gattgataaa
    13981 gcgggtggct gggctgaagt cgctaagagc actcagacag ctttagggaa actggctaac
    14041 aattgggatg atacgatggt gaaaatcttc tcaggttcac aggatgagct aaatggcttc
    14101 ttatccagcc tgagtaacct gcttagtgaa atggggatga gttcttctat cgctggtgat
    14161 gctatcggtg gacttatcga catgcttaaa gctggtgtag atgatattcg cgtattcaat
    14221 aacaccattg aaggctggat tttacagact aagaagttct attacagtct tgatgacact
    14281 aaacgaaaac tacttgatga agtcggtgat ggatttatca attttgtcaa aggtcttgct
    14341 atagcgttat ctgcaaagac actattcagt gctactacag gggtgatgaa cctgacaaga
    14401 gcaatcacaa cgttaggtac aagagctaat caggttgctg gtcaggtaac tactggtaaa
    14461 ggtggtggaa aactgaaagg tgtagctggt ggtgtaggta gtgctcttgc aattggttat
    14521 gctgatgacg gttatgaaac agcgttagca ttagcttcaa tgattccaca aatcagaggc
    14581 gtcactcttg gtttatatgc attgaagaaa gcactggatt ttatgaatca ggaagtagtc
    14641 aaaaacgcta atatacatcc atctggggta ggtgtgggta gtgactttaa tcctgtgtat
    14701 tctggtgacc ctaataaacc gaatatgatt aatgaaggat ggggtagaat ttttaactca
    14761 atgggtgaat tgtttaacaa tgccacaatg aatattgcaa gattcaatca tccagagtta
    14821 aacaacatta agcaagattc agcattaacg agtgcagata ttcagagtct aagagatgaa
    14881 attagtgctt taagtaagcg tattcaggag cctgtgaaag tttcacttgg tggtgaagtt
    14941 gccattaaac cagatgaaac cagctttatg acctttagca gtaatatcta cgaccaatat
    15001 gcagaagcta cgctattaag ttcatcattc ccagaagatg attgatttaa cggtgttcta
    15061 cgtgaataaa gcaaagttaa tacccgcact gaaaaataat ttttacgtag cacacgctta
    15121 acagagaaaa cacttatata tcaaaggctt ggttaaaaat gattaaaata cactaagcct
    15181 ttgaaatcaa aggagaattt aatgattttt agcaatcaaa tccgctacag tggcaaagat
    15241 ggcatctatt tccacttgag agataatgta gatgccttct taaccttatc agcaactgaa
    15301 aacatggaat ttgatagtcc tatgcaggtg actacgcaga acatgcaatc agggcaaacc
    15361 gtcacagata atgtgcaaag agcacccaga acaatcacta ttagcggtgt cgttgtagtt
    15421 ggctatgaag gaagcttatt attaactcgt caaggccaat tagtagagaa cttcattgac
    15481 actcttgaaa gctggagaga ccagaagcaa attatttcgg tcatctgtaa agatggtatt
    15541 aaaattgatg attccattat tacaagtttt aaagcctcta aagatgttgg tatttcaaat
    15601 ggtctgagaa tccagctaac ttttcaggaa attaacttta aagctattgt aggtcaaact
    15661 gatatttcgg ctgccactgg caaaacggct accactaatg acggtggtgt gaccagtaag
    15721 aagaatgcag gtaacgtgac cactgaacgc aataacgcgc ctatgctatc gtgcaaagag
    15781 ctaatggata ttagcgatgt atccggttac gctccagcta tcagaaatga tattctcgat
    15841 gctcagatta aatgtaataa ggcttggaag ctggactatt cagacttaac agaagctgaa
    15901 aaaaccaacg tcaccaatgc taacagtaac gctggtcgag tagcttcaaa gcttaaaaag
    15961 tactcagtga atccgaacaa gagatactaa gcgtccacct cttccatatc accagcagga
    16021 taaatccttg ttactggttc accaaaaata agcatatcat ctaatgactg catctcatcg
    16081 gttgcttctt caagcgttaa ggtgtggtca tctttaccat caagagggaa accacgaatc
    16141 atatttccat tgtgaagata tatatccacc agcttttcat taccagaaac aacaatctct
    16201 ttcaccagac gcttaataat aatctgggct tcagttctcc cctctacaga ctccatatca
    16261 agaccgctta aatccaagct tgatacagat tttaccctcg ccttttctcg tgataactga
    16321 actatcagtt catctttttc ttcctgttta acccgtagtg cttcttgaat ctgagtgatg
    16381 ttaggaagct gtatcagcgt ctcagtgagt ttttcaagtt cactatcaac ggtgtttaat
    16441 cttctttgta gttcatctaa tttagcggtc tcggtagctt catcgtgtag ctgtgatagt
    16501 ttgctaaaca gacgactaca aagctgtgta tcaagtaact ttctggacac tgtaccgtaa
    16561 ctacaacggg actccttatt accattgcaa cggtaagtac cttgataaac gggtttacgt
    16621 atccctgtag gagtcattac aagcccacac ttacaacgga taagcccctt cagtatgttc
    16681 acatatagcc agtgttcacc gctggatgtt ttccccttcc ctgtttcctc taacagttgc
    16741 tgtacagcgt aaaacttaga atctgtgaca atctgaggat agaacgattc aatttcttca
    16801 cgtaacactg ttttacggtc ttctgaagaa atcttagcgg gaaggtaacc taatacactt
    16861 cgtgaagtca ggatgtgttt aacgcttaat gctgaccagt taccatccgg tttcttagtc
    16921 ttggatattc tgaccttacg ctgattaatg gtcgggtaac cttgttcgtt tagccattta
    16981 cagatagcaa acatcgacat accagacaga cgcttgtcat agatgatatt caccagtgac
    17041 gctttatctt catcaacgat gtagtgtgaa ccgtcttctg atagctctaa ccatccgggg
    17101 gcttgcttac ccataggctt cttaagttct cttgcaagct gtgttttatg gttccagacc
    17161 tgagctatac gcttttgctt aacaagagat tcttggtgtg ctcgctcagc tatgatgatg
    17221 tgcttaatca acttcacagg gtctgttaat gctgacttgt cgtagagttc accatcaccc
    17281 aaagtcagta tagagactcc cttcctcagt atctgcctaa cgtcatcaat agctgtatca
    17341 atatctgccc tactgaagcg gtcaaagttc tctacaacaa tcaaggaacc ttctggaata
    17401 gtcccagaca tgacattgtc aaacatacgt ctgaagtcag aaccttgatt cttatgttgc
    17461 tggtgaaaac cagatttacc cttatcaata tatttgtgag cagggagaag gtaatactct
    17521 tctttatgtt tagagtagaa agcatcaatg aggtttgttt gtcgtcttag ggagtcacca
    17581 gagtcttgct ctcgagtact ccaccggatg tatgtcacta gcagcttcat atcttcggac
    17641 tatgcttatc taagtggttc attgaacagg agaacaatga tatgagtgtt aaggcaagaa
    17701 tactagtgct ttattattcc atgtacggac atattgaaac gatggcacgc gcagtcgctg
    17761 agggtgcaag caaagtggat ggcgctgaag ttgtcgttaa gcgtgtaccg gaaaccatgc
    17821 cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac tgcaccggtt gcaaccccgc
    17881 aagaactggc cgattacgac gccattattt ttggtacacc tacccgcttt ggcaacatgt
    17941 ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct gtgggcttcc ggcgcactat
    18001 acggaaaact ggcgagcgtc tttagttcca ccggtactgg cggcggtcag gaacaaacta
    18061 ttacttcaac ctggacgacc cttgcgcatc acggcatggt aattgtcccc attggctacg
    18121 cagcgcagga attatttgac gtttcacagg ttcgcggcgg tacgccgtac ggcgcaacca
    18181 ccatcgcagg cggtgacggc tcacgccagc caagccagga agaactgtct attgctcgtt
    18241 atcaagggga atatgtcgca ggtctggcag ttaaacttaa cggctaatct tcaacaggag
    18301 gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc ggtgaatggg catctctgcg
    18361 caatacgtcg ccggaaatag ccgaggccat tttcgaagtc gccgggtacg acgaaaaaat
    18421 ggcggaaaag atttgggaag aaggtagcga tgaagtgtta gtcaaagcct ttgctaagac
    18481 cgataaagat tcgctttttt ggggcgaaca gaccatcgaa cgtaaaaacg tttaaccagc
    18541 gactcccccg cttctcgcgg gggagttttc tgttatttca ccgcttcatt caacacgcta
    18601 tcaaacttat ccatcgggca gaaaccgttg gcatctatcg ggcaaccgct taattccagc
    18661 gtcacacgct gcgcaggtgc ctgcagggtt aacgcatcgg cattacgtaa ctgttccgca
    18721 ctctggtaca catattcaat tttcatcaaa tcgcgattgg ctttgctgtc atgccaacgc
    18781 tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct ggtcatgcaa ctgatacggt
    18841 ttgaaatcca gcgccgttaa cagagaggct atgttggagt cgtgcccaac taacaccgtg
    18901 attttcggag cactggcgcg atcggtgacc agggctttgt cgatataact gaccagcggt
    18961 ttcgcaacat tgcgcgccac ttccggtgag gtaaacaggc tgtcctggta accgtttttc
    19021 agcttcgaca acaccttcca ctgctggtca gatttgattt ctccccaggc cacctgatcc
    19081 atcggaaaac cttcgtagta ttgcaaagta aacgcatcta ccagcgagtt gccgactttc
    19141 agcggcccgg aaacacctgg ttcttgttga tacttcgcgc taaaggtatt tttgccatcc
    19201 accagcgaac actgttgttt ctctttacag gcaggggaat ctttatagtt aacgattttt
    19261 tccagtagct ggtagctgtc ggtaagctgg agtttgctga gctctttctc cattgccgcc
    19321 accgcctttt cactgaatgc ggcggaatca tcggtgatca ccgggttaaa ggttgggtcc
    19381 atggtgccca ttttttcctg gtgatgcaca ggaatatcac accccgggaa tgc
//
LOCUS       DAOFQD010000088        19327 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.088, whole genome shotgun sequence.
ACCESSION   DAOFQD010000088 DAOFQD010000000
VERSION     DAOFQD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.088"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(56..1411)
                     /locus_tag="QBF46_003219"
     CDS             complement(56..1411)
                     /locus_tag="QBF46_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDQ6675369.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSSDDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVTMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(1415..1699)
                     /gene="gatB"
                     /locus_tag="QBF46_003220"
     CDS             complement(1415..1699)
                     /gene="gatB"
                     /locus_tag="QBF46_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDQ6675370.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGIGIEALQNKILTILQG"
     gene            complement(1730..2182)
                     /gene="gatA"
                     /locus_tag="QBF46_003221"
     CDS             complement(1730..2182)
                     /gene="gatA"
                     /locus_tag="QBF46_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDQ6675371.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(2192..3454)
                     /gene="gatZ"
                     /locus_tag="QBF46_003222"
     CDS             complement(2192..3454)
                     /gene="gatZ"
                     /locus_tag="QBF46_003222"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDQ6675372.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(3483..4337)
                     /gene="gatY"
                     /locus_tag="QBF46_003223"
     CDS             complement(3483..4337)
                     /gene="gatY"
                     /locus_tag="QBF46_003223"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDQ6675373.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPSTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(4645..5697)
                     /gene="fbaB"
                     /locus_tag="QBF46_003224"
     CDS             complement(4645..5697)
                     /gene="fbaB"
                     /locus_tag="QBF46_003224"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDQ6675374.1"
                     /translation="MTDIAQLLGKDADNLLQHHCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            5954..7231
                     /locus_tag="QBF46_003225"
     CDS             5954..7231
                     /locus_tag="QBF46_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6675375.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGTVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            7228..8232
                     /locus_tag="QBF46_003226"
     CDS             7228..8232
                     /locus_tag="QBF46_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDQ6675376.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            8229..9194
                     /locus_tag="QBF46_003227"
     CDS             8229..9194
                     /locus_tag="QBF46_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDQ6675377.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNTLPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNHQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9168..9914)
                     /locus_tag="QBF46_003228"
     CDS             complement(9168..9914)
                     /locus_tag="QBF46_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDQ6675378.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(9966..10784)
                     /locus_tag="QBF46_003229"
     CDS             complement(9966..10784)
                     /locus_tag="QBF46_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDQ6675379.1"
                     /translation="MQLRITSRKKFTALLCALGLISIVAICPRQTVNFFYSTAVQIKD
                     YIHFYGYRPVKSFAIRIPATYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVAPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(10849..11649)
                     /gene="thiD"
                     /locus_tag="QBF46_003230"
     CDS             complement(10849..11649)
                     /gene="thiD"
                     /locus_tag="QBF46_003230"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDQ6675380.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(11646..12434)
                     /gene="thiM"
                     /locus_tag="QBF46_003231"
     CDS             complement(11646..12434)
                     /gene="thiM"
                     /locus_tag="QBF46_003231"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDQ6675381.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(12469..12565)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12657..12929)
                     /gene="rcnR"
                     /locus_tag="QBF46_003232"
     CDS             complement(12657..12929)
                     /gene="rcnR"
                     /locus_tag="QBF46_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDQ6675382.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            13050..13874
                     /gene="rcnA"
                     /locus_tag="QBF46_003233"
     CDS             13050..13874
                     /gene="rcnA"
                     /locus_tag="QBF46_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDQ6675383.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEHHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            14093..14431
                     /gene="rcnB"
                     /locus_tag="QBF46_003234"
     CDS             14093..14431
                     /gene="rcnB"
                     /locus_tag="QBF46_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDQ6675384.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(14513..15547)
                     /locus_tag="QBF46_003235"
     CDS             complement(14513..15547)
                     /locus_tag="QBF46_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDQ6675385.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKKEDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNIILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(15563..18043)
                     /locus_tag="QBF46_003236"
     CDS             complement(15563..18043)
                     /locus_tag="QBF46_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6675386.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(18059..18733)
                     /locus_tag="QBF46_003237"
     CDS             complement(18059..18733)
                     /locus_tag="QBF46_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDQ6675387.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(18814..>19327)
                     /locus_tag="QBF46_003238"
     CDS             complement(18814..>19327)
                     /locus_tag="QBF46_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6675388.1"
                     /translation="FSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINM
                     PTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIAS
                     SNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVL
                     SSLVMRVAQD"
BASE COUNT         4853 a         4917 c         4479 g         5078 t
ORIGIN      
        1 gatttcataa aaactcctga ttgttaaggg ggataacctc cccctcaggt aaaaattatt
       61 ctgcgagaac gactttctct tgtttaataa agccacgcgc tctacgccag gtcatgaaaa
      121 taccggtaag ataaattgcg ccgataataa tgaatccggg aatattttgc ggggagaaaa
      181 cctgaatcag taaccaggta atgggagaac cgccctgatc cattgaagcc accatacccc
      241 cggcttttaa cgccccagca ttagccgcca gttgggtgtg caaaccaata gtttgcgtcg
      301 cgatccacag ggtgatactc ataatgatga cacccgagat taaggtgcgg aacagatttc
      361 cacgatgcac cgcgaccgcc atcgtcacga agaagccgat agtggcaaga tcgccaaacg
      421 gcagcacctg attacccggc acacaaacag caattaaaat ggtgagcggg ataaaaatca
      481 ggcttgccga taccaccgcc gtatgcccca gcagtaatgc gggatccagg ccaatcagga
      541 actcctgacc gccgaacttc gcctgtaaac gactacgggc ctgcttagcg atgggcgtta
      601 aaccatccat gatgggttta atcacccgtg gcatcagcag catcaccgcc gccgttttta
      661 ccgccagctg caatacacct ttgacatcgt aacccgcgag gatgccgata atcagcccca
      721 tcacaaaacc cacggtgaca ggctcgccaa acggaccaaa tttgcgctga atatcgtcgg
      781 agctaaattt aatacggttt acgcctggga ttttctcgat aatggcatcg accagcaccg
      841 caatcggccc catatacgcc gacgtaccgt gcggaatagc aatgccttcc agctcaaaga
      901 aatttcgggt atcgcgggca aaccagtcgc cgagcttata aacaaacgcc gcgtgaatta
      961 ccacacccgc catccctatc atccatgaac cggttgccag atgcagcaac gcgccggtga
     1021 aggtcatatg ccagatattc cagatatcaa catttaccac ccgcgtcata cgggtcagta
     1081 acatcgccac gttaaccaga atcgcaatcg gaatcgccac cagcgcaatt tgcgacgccc
     1141 aggtcattgg tgaagagccc ggccagccaa catcgaccac atgcagattc aggtcgaaat
     1201 tttccgccat cgctttcgcc gccgggccaa tggaatccag cattaagcca atcacaaggc
     1261 caatgccaac aaagccaatc ccgatatgca gacccgcttt aaagcaatcg cctgccttca
     1321 tgcctaatat tttagaaaaa ataatgatga caatcggcag catcaccgta gggccgaggt
     1381 cgagaatata acgcatgact tctgaaaaca taggtcaccc ctgtaagata gtcagaattt
     1441 tattttgtaa tgcttcgata ccgataccag aaataaaagg catgccgtga actaacggaa
     1501 tatcgccaaa actacgatcc actttggcag tggtgcatat caaatgcaca ccatccatat
     1561 aggtttctat ttcattaacc cgacactgga ttaattcaac aggaatatta tgattctgac
     1621 acaactcttt aatttcttcc gccgccatcg tagaggtcgc aaccgcgcct ccgcaagcga
     1681 caataatctt gcgtttcata aatagtcctt tttattgtta cagagggatt tattcgtctg
     1741 aatctaaaac atactttgtg aagtattcct ttaactgggt ttcaggaaga gtgattagtg
     1801 tctcgacgat atcgggctgt tgtaacttgc caaataaaca gcgtaaaagt ttcaattgct
     1861 gctgcggatt ttccacaatc aacgcaataa ccaacgatac cgccacgtcg ttatcatcat
     1921 ccgcttgctg aaaatgaact ttatttgttg gccttaacag ataaatggct gacgacttag
     1981 catgaatcgc ctcacaatgc ggtattgcaa tagcgtgctg ctcaagcatt atcccggtag
     2041 ggaattctgc ttctctggca attaatgcct gtggccaggt atcatgaacc acacctttgg
     2101 cgagcatctc attaccgata tgggttaaaa cttcgctacg atcgacaaaa gaaattccgc
     2161 tacgaacaaa caggttagtc atataccgtc cttattccgc acagccgtag cgataggcgc
     2221 gcaaaacatc ataaatttta tccataatca gctgatgcgg tattgctgat aattccccgg
     2281 actgaatgcg ttcaaattgt ttgggaagat actgactaat catgcccagt gggatgtcca
     2341 cgccttcaag attcaccatc atcgtttcga cgctattttt aatccgacta tgcggccagt
     2401 aataacgaat acgatccgac aggctgtaac gaatatccag taatgaatcg ttaaaacccg
     2461 tacgataata ttttttccag tattgcggtt cgtcgagcat cacttcttca attaccgcca
     2521 ggcaaccgct gcgattttca ggggcgataa gttcctgctc aatttgtgcc agtgcaaata
     2581 tcgcctcgcg taaagcaaag gttaatgcgg gaccgacttt caatattgca aagtgatcgc
     2641 ggactaattc ccaataagcc gtccgggtct ggtaatcggt agaatgtgct tcataaacca
     2701 ttcgggtgtt ttctatccat tgcgccagcg gctgcgcttc ctgcggctga taatggataa
     2761 tattgctgtg atcaaattcc acgcccggct gcaccacgat ggcaatcacg cgtgttaacg
     2821 cctctgtcag cccacgggca ataaaggcct tttgatgcgt acgtaaagta ttggcggcat
     2881 cttcaacatg ggtgatgtgt actgactgaa tggcgctggc ctcaccgccc ggaaccggaa
     2941 cttcggtgcc aatgacatag ctcagttgct cacgctggca atctgtcgcc acactttccg
     3001 cagcaaagca aagcacagca gctcgttccg caaccgtttc tggtgctaac ggtatgggat
     3061 cccccgcgca ggacattgac gcatcaagat gaattttact gaagccggca cgaacatatt
     3121 cctttaccag ctcgacggat ttttccatcg ccgcatccgc attttcttgc tgccagcagt
     3181 ttggccccag atgatcgccg ccgagaataa tgcgttcgcg tgcaaaccca actttatcgg
     3241 caatcgtaaa aacaaattcg cgaaagtctg ccggtgtcat tccggtataa ccgccaaatt
     3301 gattgacctg gtttgacgtt gcttcaatca gcactttgcg cgtgctgttg cgatcaaatg
     3361 ccagcgccgc ttcgataacc aacggatggg cagaacagac tgaacatatg ccgatatgtt
     3421 caccagcttt atgccgggca attaacgttt tcatgttttt tccttgttaa atggcaggtg
     3481 cgttatgccc tgccctcgca gccacaatcg gcaatcactt tgctcaccac atcgcgcatt
     3541 gcggatttag ccgactgcaa ataatcccgg ggatcggtcg cttcagggtg ctcggtcagg
     3601 taatttttta acgcctgcga gaaggcattt ttcagctccg ttgcaacgtt gattttgcat
     3661 atccccagtt tgatggtttg ctgaatatcc ttagtcgata accctgacgc gccatgcagc
     3721 accagcggta agttcaccca ctggcgaatg ttctccagtc tagaaaaatc aagcgccggt
     3781 gcgctggcat acatcccatg agccgtgccg atggcgaccg ccagggaatc aattccggtt
     3841 gcctcggcaa attcacgcgc ctgagcgggg ttggtgtaca acgcatcggc ttcattgact
     3901 tgcacatcat cttcctggcc gccaagttgc cccagctccg cctcgacgct gacatcaaag
     3961 cgatggcaaa aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa aggcaaatgc
     4021 gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct tctgagcgat atcgtcaaat
     4081 ttcgtgtgat ggtcgagatg aattgccagt ggatggtgat attgcttcgc catcgcgctg
     4141 accagcgcca acagattttc tgtaccagca tgagtaaatg tgctaggcgt tccggcgatg
     4201 atgaccggcg catgcaggtt ggcagcggtt tctaccacca cttgcatcgt ttcgagattg
     4261 tgaatattga atgccggaac cgcataaccg ccgcgctgtg cgttgttcag catctgcttt
     4321 gtcgatacca cgtacatttt catatcctgt cgttttcttt cgatttcaaa atataatgaa
     4381 attatttgtt tttaaatgtc gagataacga tcacaaaaac gacaatatga aaattgttcg
     4441 aggagtgaaa ggcaaaaaaa cggcctcccg atagggaagc cgtagcaaag tgcgcgtgtt
     4501 tttatgccgg atgcggtgta aacatacgtg gcagtgctat gtaggcatga taaggcgctt
     4561 ccgcgtcgca tcaggcatag gttgccggat gcagtgtaaa cgccttatcc gtcctacggg
     4621 gtggtgctgt tcgtatgagg cgtatcaggc aatagtaatt ttgctatcga gataaacgtc
     4681 ctgcacggca ttaatcagtt tcacgccgtc agccatcgat ttcttgaacg ccttacgtcc
     4741 aagaatcagc cccattccgc ctgcgcgttt gttgataacc gcagtacgca ctgcatcgct
     4801 gaggtcagtt tcaccgcctg cagcaccgcc ggagtttatc aacccagccc ggcccatata
     4861 gcagttagct aactgataac gcaccagatc aatcgggttt tcgctggtta acttgctgta
     4921 cacgcgatcg tcggtataac cgtaattaat cgctttatag ccgccgttat tttccgccat
     4981 tttttgtttg acgatatctg cgccaatggt cgccgccaga tggtttgcct gaccggtcag
     5041 gtcggcggaa acatggtaat caacgccatc tttcttaaag gcggagttac gcaaataggc
     5101 ccacagcacg gtcaccatgc ccagctcgtg cgcacgttca aaagccgcag aaatttcttc
     5161 aatctggcga cgtgactctt ccgagccaaa atagatagtc gcaccaaccg ccaccgcgcc
     5221 catgttgaac gcctgctcca cgctggcata cagcgtttga tcgtaggtat tcgggtaact
     5281 tagcgtctcg ttgtgattaa gtttgacgag aaatggaatg cgatgcgcgt aacgccgcga
     5341 taccgacgcc agcacgccgt aagttgacgc cacacagtta cagcccgctt cgatcgccag
     5401 ttcaacaatg tttttcgggt caaagtagag cgggttagca gcaaatgaag ctccggcaga
     5461 gtgctcaacg ccctggtcaa cgggcagaat agaaagatat cctgtgccag ccagacgccc
     5521 ggtgttgtac aacgtctgca tattacgtaa caccgctggc gggcgattat tgtcaatcat
     5581 cacgcggtct acgtagtcat gtccggggag ataaagctgg tcagaaggaa tagtcataca
     5641 atggtgctgt aaaaggttgt cggcgtcttt gccaagcaac tgcgcaatat ctgtcattac
     5701 tatgctcccg taaattccga ttggatatcg gctatggatt gtcctggccc gccttttgcg
     5761 ggcaatcata atcctggtcg ttacgggcga gtttttccat cttttggatg ttttttcagc
     5821 gttttctgtt ggctcgattc atcagaaaaa atgttaccgc ggtcaagttt tcaccgcaaa
     5881 ggtatttaaa aggtattatt aagtggtatt gtcatcgcgt accttacatt acctgtcatg
     5941 aaggaattaa aagatgaaaa caacagcaaa gctgtcgttc atgatgtttg ttgaatggtt
     6001 tatctggggc gcgtggtttg tgccattgtg gttgtggtta agtaaaagcg gttttagcgc
     6061 cggagaaatt ggctggtcgt atgcctgtac cgccattgcg gcgatcctgt cgccgattct
     6121 ggttggctcc atcactgacc gctttttctc ggcgcagaaa gtgctggcgg tattgatgtt
     6181 cgctggtacg gtgctgatgt atttcgctgc gcaacagacc acttttgccg ggttcttccc
     6241 gttactgctg gcctactcgc taacctatat gccgaccatt gcgctgacta acagcatcgc
     6301 ttttgccaac gtgccggatg ttgagcgtga tttcccgcgc attcgtgtga tgggcactat
     6361 cggctggatt gcctccggtc tggcatgtgg tttcttgccg caaatgctgg ggtatgccga
     6421 tatctcaccg actaacatcc cgctgctgat taccgccgga agttctgctc tgctcggtgt
     6481 gtttgcgttt ttcctgcccg acacgccgcc aaaaagcacc ggcaaaatgg acattaaagt
     6541 catgctcggc ctggatgcgc tgatcctgct gcgcgataaa aacttcctcg tctttttctt
     6601 ctgttcattc ctgtttgcga tgccactggc gttctattac atctttgcca acgggtatct
     6661 gaccgaagtc ggcatgaaaa acgccactgg ctggatgacg ctcggccagt tctctgaaat
     6721 cttctttatg ctggcattgc cgtttttcac taaacgcttt ggtatcaaaa aggtattatt
     6781 gcttggtctg gtcaccgctg cgatccgcta tggcttcttt atttacggta gtgcggatga
     6841 atatttcacc tacgcgttac tgttcctcgg tattttgctg catggcgtaa gttacgattt
     6901 ttactacgtt accgcttaca tctatgtcga taaaaaagcc cccgtgcata tgcgtaccgc
     6961 cgcgcagggg ctgatcacgc tctgctgcca ggggttcggc agtttgctcg gctatcgtct
     7021 tggcggtgtg atgatggaaa agatgttcgc ttatccggaa ccggtaaacg ggctgacttt
     7081 caactggtcc gggatgtgga ctttcggcgc ggtgatgatt gccattatcg ccgtgctgtt
     7141 catgattttt ttccgcgaat ccgacaacga aattacggct atcaaggtcg atgatcgcga
     7201 tattgcgttg acacaagggg aagttaaatg aaaacagaac gtattctcgg tgctctttat
     7261 gggcaggcgt taggggatgc gatggggatg ccctccgagc tttggccacg cagccgcgtt
     7321 aaagcacact ttggctggat tgaccgtttt ctgcctggac caaaggagaa taacgcggcc
     7381 tgttatttta accgcgccga attcaccgac gatacctcga tggcgctgtg tctggcggat
     7441 gcgttgctgg aacgtgaagg caagatcgat ccggatctga ttgggcgtaa tatcctcgac
     7501 tgggcgctgc gtttcgacgc cttcaacaaa aacgtgctag gtccgacctc gaagattgcg
     7561 cttaacgcca ttcgcgacgg taaacccgtt gctgaactgg aaaacaacgg cgtgaccaac
     7621 ggcgcagcga tgcgcgtctc gccattaggt tgtttgcttc cggcgcgcga tgttgattcc
     7681 tttattgatg atgtggcgct ggcgtccagc ccgacccata aatccgatct ggcggttgca
     7741 ggtgcggtag tcatcgcatg ggcgatttct cgtgccattg acggagaaag ctggtcagcg
     7801 attgttgatt cactgccttc aattgcgcga catgcacagc aaaaacgcat cactaccttc
     7861 agcgcctcac tggcagcacg tctggagatt gcgctgaaaa ttgtgcgcaa tgccgacggc
     7921 accgaatccg ccagcgaaca gctttaccag gtcgttggcg caggtaccag cactattgag
     7981 tccgttccgt gcgccattgc gctggttgaa ctggcacaaa ccgacccgaa ccgttgcgcc
     8041 gttctctgcg ctaaccttgg cggcgacaca gacaccatcg gtgctatggc gacggcgatt
     8101 tgcggcgcgt tgcatggcgt taacgctatc gatcctgcgt taaaggcgga actggatgcg
     8161 gtaaatcagc ttgatttcaa ccgctatgcc acggcgctgg cgaaatatcg tcaacaacgg
     8221 gaggcggtat gagcggcgct cgattacaca cgctgctgcc tgaattaacc acgcgtcagc
     8281 cggtgatggt cgtcggcgcg gcggtcattg acgtgatcgc cgacgcttat gccctcccct
     8341 ggcgtgggtg cgatatcgaa ctgaaacagc agagcgttaa cgttggcggc tgcgcactga
     8401 atattgccgt ggcgttaaag cgcctcggca tcgaagcggg taataccttg ccgctcggtc
     8461 agggggtatg ggcggagatt attcgcaacc ggatggcaaa agagggctta atcagcctga
     8521 tcgataacgc cgaaggtgat aacggctggt gtctggcgct ggttgagcct gatggcgaac
     8581 gcacttttat gtcattcagc ggtgtggaaa atcaatggaa tcaccaatgg ttggcacgat
     8641 taaccgttgc gcctggcagc ctgctctatt tttccggtta tcaactggcc tcgccctgcg
     8701 gcgaattgtt agtggaatgg ctggaaaagc tgcaagacgt gacgccgttt atcgattttg
     8761 gcccacgtat tggcgatatc ccggacgcat tactggcgcg gatcatggcc tgtcgacctt
     8821 tagtatcact caatcgtcaa gaggctgaga ttgccgccga acgttttgct ttatccgcag
     8881 agataacaac acttggcaaa caatggcagg agaaatttgc cgcgccgttg atcgttcgtc
     8941 tcgataaaga aggcgcatgg tatttcagca acgacgcttc tggctgcatt ccggcatttc
     9001 caacgcaagt tgtcgacacc attggggcgg gcgacagtca tgccggtggc gtacttgccg
     9061 ggctggcctc tggtctgcca ctggcggatg ccgtattact gggcaatgca gtggcgtcgt
     9121 gggttgtcgg gcatcggggc ggtgattgtg cgccaacgcg cgaggaacta ctcctcgcac
     9181 acaaaaacgt atagatcgct gcgacagtgg ctaatgctgt attcaatagg ccgttgctgt
     9241 tggtcaagcg ccacttgctt gatcaccagc accggtattt tgctatctaa ctgaatatgt
     9301 gactgaaact cggcatctgg catccgggcg ctaacgcggg aacgtgtacg ttgtgggtaa
     9361 atgtgctggc tgcggaaata gtcataaaga gaaattccga tggcatcaac atcatgaatt
     9421 aaatgcgccg gaacccacga ttcctcaatc gataccgctt cttcatcgac atagcgaata
     9481 cgcttaagca aaaagacatc acttcccgct tcgaccgcca gctgctgcgc gacttcttcg
     9541 ggacatttca cgacacgctt gttaacccat agcgtatcgg gctttttccc gcgcaatacc
     9601 acctgctgag aaaaacctcg cgcttctttc aacgagtatt cgaagatgtt gttgatctgc
     9661 gtgccgtaac cacgcgaacg cgtcactacg ccctcttctt ccagcgcctg catcgccttg
     9721 cgcaccgtaa tgcgcgaaac gccggttaac tgacttaaat cacgctcacc gggcaaaata
     9781 ttgccatgct ccagcacccc gctgcgcacg gcatttttta ccgtttcggc aaacttaata
     9841 taaagcggcg tgttatccgc cgctaaaata cgctcgttca gttgggcaat cagctgggta
     9901 tgcgcttgtt ccatttgtct ttttcctgcc agggagtctg gtgggagtat atcactgctt
     9961 cttatttaag gcgtttcttt aatcccatca acgaatgcct gcaactcctc caccgtgcca
    10021 ttaaacacat taaaatccac cggaccattg atgccatcta cctgtccacg gtcggaatgc
    10081 tgccagaagc gccaggccat gccgtcattg tccggacgac gttgataata gtgagccacc
    10141 caccacggat attcattgaa atagcccgcc agattggtgt gataaaaaac ggctcctgag
    10201 taaataatcg gctttttccc cgtacttttt tcgaccattt ttagccactg acttacccgc
    10261 ttgcgtaatt cttttgccga taattttccc cgttcttcta cgtccagtac cgcagggaaa
    10321 tcgccttgtg agaaatccac cgtttgcaga aataatcgcg cctgaactga agcggatacc
    10381 gacggggaga aataatgata cgccccgcgc agcaggccat tttcgcggct tagttgccag
    10441 ttacgcgaaa aatagggggc caccagcttt tcgccttccg tagccttaat aaaagcaaac
    10501 tgtaagcgga taccgttgtc gcgcattttt gccacccgct gccagtcgat ccgctcctgc
    10561 cagcgcgaaa catctattcc gtgaatggtg taagtggcag gaatgcgaat agcgaaagat
    10621 ttaaccgggc gataaccgta gaagtggatg tagtctttaa tctgaactgc tgtcgaatag
    10681 aaaaaattca ccgtctgacg tggacaaatt gcaacgatgg aaattagccc aagagcgcat
    10741 aataaagcgg tgaatttttt tcgactggtg attctcagct gcattggttt catccctgaa
    10801 tgtcagtgcc aaagactgac aataaccaaa gcagctatag tacggtgctc accaccaggc
    10861 gtggaagtgg tgaaccggac caataccgtg accaacttcc agcgtgtcgg cctgggctaa
    10921 cgccgaggaa agccagcttt ttgcctcctg tacggtgtca gcccagtttg tatggcgcgg
    10981 gcgtagtgca gccaacgccg cagagagtgt acaaccagtg ccgtgggtgt ttttggtcat
    11041 aatgcgcggt gcggtaaacc gttgttcacc ctcgcgggta aacagccagt ccgggctttg
    11101 ctcatcatcc agatgaccac ctttcattag cactgcgcca cagcccatcg ccaacagcga
    11161 tcgcccttgt tccagcattt cctgttcggt gcgcgcgtgt ggcgcgtcga gcaaggcggc
    11221 agcttcgggc aagtttggcg ttattaatga aacctgtggc aataatcgac tgcgcagcgt
    11281 agcaaccgcc gaaggtgaaa gcagcgggtc gccgcttttt gccagcataa cggtgtcgag
    11341 taccacgttt tggatctgat aacgttgcaa ccgttctgcc accgcttcaa caatatcggt
    11401 ttccgccaac ataccgattt tagtggtatc gattcgcaca tcgctgaaca ccgaatcgag
    11461 ctgggcggcg acaaaatcag gctcaatgcg atacaccgac tgtacgccac gggtattttg
    11521 cgccaccagt gcagtaataa ctgagcaacc ataagcgcca agtgccgaga aggttttaag
    11581 atcggcctga atccccgcac caccactcgg atcagtaccg gcaatcgtca gagcgttaat
    11641 tcgtttcatg cctgcacctc ctgcgtcaat tgccagagcg catcaaggaa atgtggaaca
    11701 aaactgcctg gcccctcgct tctggcgact gcgcgctctc cggcttgttt catccagtga
    11761 caggcagatg cgacattttc cagcgtatcg cctggtaacg cacagcaggc agcgacaacc
    11821 gccgataatg cacagccagt tcctaccact ttggtcatta acggatcgcc accgtgaatg
    11881 ccaacggcgc gatggccatc ggtaacataa tccacctcgc cagtgaccac gacgattgcg
    11941 ccagtttccc gtgccagtgt ttgtgcagcg ggtatcgcgt tagctgcggc gtcagtggta
    12001 tccactcccc gtccaccatt agcaatgcca gctaatgcca tgatttccga agcattacca
    12061 cgtatcgctg ccggtttaaa agataaaagt tcatgacaaa aatggcggcg ataatcgagc
    12121 gcaccaaccg ctactggatc aagcgtccag ggtgtttgag agctttttgc ttgctcaacg
    12181 gcagcacgca tcgcctgagc gcgtggctgc gtcagtgtgc caacgttaat caacaaggca
    12241 ctggcgatag ccgcaaactg actggcctct tcggtttcga taaccatcgc tggcgatgca
    12301 ccgagcgcca gcaaggtatt ggcggtaaag gtttgcacca catcattggt catgcagtgc
    12361 acaagagggg aatgttggtg aaaaaggtgt aacgcgtgcg cagattgcgc tgaacccagc
    12421 aggtcgactt gcatagtttg ctcctgccat aacgtgaaga agcaatgacc tggtggtccg
    12481 tgacttccct acgctggcat tatccagatc aggtgatacg ggtatttctc agccttcacg
    12541 cagaagggca ccccgagtcg tttggttgcg atgataaatc gcagaggagg atggtaatgt
    12601 ccagcgcacg cgttgtaaac gaggaaaaac ggcagtttta caatcgcgtt aataaattat
    12661 ttgatatatg aatccagcac cttcagaacg acatccagat cttcttcacg ttttagctca
    12721 tccccctggt gaacgatgtg ttccgtcaga tgacctttaa tcacttcccg catcagaccg
    12781 tttaccgcgc cacggatagc agcaatctgt tgtaaaactg cagcgcattc gtgcggctcg
    12841 tcgagcattt tcttgagcgc cacgacctgg ccctgaatct tactggcacg cgctttcagt
    12901 ttctgtttat cacggattgt atgagacatg gcaacacctg gttaacaaga atatgaaaaa
    12961 tcatagcact attaatctac tggggggtag tatcaggtac tgggggggag tagaatcaga
    13021 ttgccgaatt aatactaaga attattatca tgaccgaatt tacaactctt cttcagcaag
    13081 gaaacgcctg gttcttcatc cccagcgcca tcttacttgg tgcgcttcat ggcctggaac
    13141 cagggcactc aaaaacgatg atggcggcgt ttatcatcgc catcaaaggc accattaaac
    13201 aagcggtgat gctcggactg gcagcaacta tttcgcatac cgcagtggtc tggttaattg
    13261 cctttggcgg gatggtgatc agcaagcgct ttactgctca atcagcagaa ccgtggctcc
    13321 agctgatttc cgcagtgatc attattagca ccgcgttctg gatgttctgg cgtacctggc
    13381 gcggcgaacg caactggctg gagaatatgc acgggcatga ttatgagcat catcatcacg
    13441 atcacgaaca tcaccacgac catggacatc atcaccatca cgaacatggc gagtatcagg
    13501 atgcccatgc acgagcccat gccaatgaca ttaaacgacg ctttgatggt agagaggtca
    13561 ccaactggca aattttgtta tttggcttaa ccggtggcct tatcccctgc ccggcagcaa
    13621 ttaccgtgct gttgatatgc attcagttga aagccctgac actgggcgca acactggtcg
    13681 tcagtttcag cattggcctg gcgttaacgc ttgtcaccgt aggcgttggc gcagcaatca
    13741 gcgttcagca ggtcgcaaaa cgctggagcg gatttaacac tctcgctaaa cgcgccccct
    13801 atttttccag tctgttgatt ggcttagtcg gtgtgtatat gggcgtacat ggcttcatgg
    13861 gcataatgcg ataactcaat cctttaggct tgcatgttat cttcagccca ggatgattct
    13921 taaatcagct atttccgctg actctctgct cgctaaggat gcttttaggg catcctttca
    13981 tttacacttt ttacgaaatc atgggatcac taacaaaata tcgcttgtca gttatattgt
    14041 atggcaggaa agatatgcga ctgatattac agatccccaa agtggagagt ttatgaccat
    14101 taaaaataag atgttgctgg gtgcgctttt gctggttacc agtgccgcct gggccgcacc
    14161 agccaccgcg ggttcgacca atacctcggg aatttctaag tatgagttaa gcagtttcat
    14221 tgctgacttt aagcatttca aaccaggcga taccgtccca gagatgtacc gcaccgatga
    14281 gtacaacatt aagcagtggc agttacgtaa cctgcccgcg cctgatgccg ggacgcactg
    14341 gacctatatg ggtggcgcgt acgtgttgat cagcgacacc gacggtaaaa tcattaaagc
    14401 ctacgacggt gagatttttt atcatcgcta aaaaaagccc cctcatcatg agggggaaat
    14461 gcagacacct tgttattttt tattattagc cacttgctcg tcttgcttgt tattagtcgt
    14521 atttcacgtt gattaatgcg gttgcctcca gtgcgccaga tttaatttta tttgtatcgt
    14581 agacgtagta actggctgtt atcggaattg aaaccgttgt atctgtaagt tttgactttg
    14641 ccgcaatttc ttttaaattt gtgtatcctt tactgctgtt agtgatattt aaaagtgtga
    14701 ctggcgaacc attactttca agaataaatc ctacgccatt atcttccgca cctttcagta
    14761 ttatgttgct gttggttaac aagtcagaac ttgataaata cacttgcatt ttccgtgatg
    14821 tttgattttg ttgacctaat aaatttttac aacgcagaat aatatcctct tttttactat
    14881 ttattgttgc tttgttacct gtcgctggta attgtgaaac gggaatactg tctaaagtaa
    14941 ttaataaatc gtccggtttg caggttgtgg ttatcgggtt gtatgacaat gtcagattaa
    15001 aggaatagac acctgcattt tgtgccaatg catttctgta gctatttaca catgcatccc
    15061 atgacttgcc acctaataaa cacagtagaa attgtactgc ggcactcccg cgtgtcgatg
    15121 atgaagcact ggataatgtc gcaactccat taatcatcac tgaaccgccg ccccccgcag
    15181 aaacattcac ttttgcatta ctggccgcag gtgtcaccgt taccgaataa ccactggtga
    15241 aactggtctg attatttgac aacgtgatgt tgtttttatt ggtccattca aaatgtaaat
    15301 ttaatttaat ggtatcgtta taaagtccaa caataatatc cgacgggctc ataggggtga
    15361 tagtatttgt actcacgcat gacgaatcat taaaattatg ctcatatgtc gccatttgtg
    15421 tttgagtaag tgcaaaatcc ccctgaacaa ccaaattgtg ctgaacagat aactcactat
    15481 aacatgccgc atagctcaca ggcataacca tcatcattaa tataaatagc attatgcgaa
    15541 tttccataga accacctgac tattactggc aaatataatt tctactttca tcaatctctt
    15601 taccgaaggt gattgtgcat gaaagtcctt gttgcttatc aattgccaca ttaaccgatg
    15661 gcggtacttc attggtgcga ataaataact ggcttccctg gccgacaacg ccaatattat
    15721 gaccatggat atcattgact tcataaccaa acattaatgg ttgaccatcc gctcttaacg
    15781 ctttaataaa ccagggcttg cgctgatcgg tatcaaaatt aaccagtaca accgcgccgc
    15841 gataaggggc ggcaattttc cggttgccac gtaattctgc ttcgctatcg ctttgcgaca
    15901 catccagcat caggtgattt tcccgataag gtgtcattcc gtcgtatatc accactccat
    15961 tacggtttgt tgtgcgatat ttttgcccat tgacataagc atctttaatt cctggcgcat
    16021 tcatcacagc aaacgtttcg gaaagacggt tcgccagatt aacgccaccc gaccaggcga
    16081 caatgccccc tgaaacactg gctccagcct gtcgataagt actcgactga ctataactgc
    16141 cattcactgt cgcaaccggc gcgttccagg tcaaattcgc cccagctgtc gtttcatttc
    16201 cctgatgttg atgactcagg ttgacaccat aattgaactg atcccgactc cctactgttc
    16261 ctgataatcc cgtattattt gaggcaaacc cctgatcatc aaacgtcgtt gagttagaca
    16321 tatatatttg ccgacgaggc gtcgaaacgt catcacccca atcaaaggga atcgatataa
    16381 aaatattaaa acgtttctct tcatgatgat tctcgtcata agcctggctt gccgcgaggg
    16441 tatagcttat ccgtcgcagg ttgttggaat aactcaactg ataatcctta ctactgccgc
    16501 tacgccccca gtaatctcgc cataacgtac ttaatgacac agacccccaa ccttctggca
    16561 atgactggct catattggcg gaaaagctat ttttgcgacc aaaatcattc tggtaataat
    16621 cggcaatgtc atagacatcg ttttcatcac ggcgataatt atctttattg tttgcccaaa
    16681 cgtgatcgtt aaatgtccgg taatcacgcg acgaataacg ccaggccgcc agaccaaaac
    16741 gcgtcgacgt ttggctcaca aatttgttgt aggcaatttg ataactttgc ccgtcaaaca
    16801 catcgccgtt gtcttgttta ctatgcgact tcgtggcatc gacggaaatg gcaccaatgc
    16861 gtgtattcca gccagtcccc aaagtaaagg catagtaatt attagcaacc atcgtgccgc
    16921 catacagcgt caataagtta ttaaaaccat actgataacc tgcctggaca aaatcacttt
    16981 gcttgctcgc accttcaata tgactacggc ccgctgcaaa atcatatttt gatacgccag
    17041 gttgcaacat attaggtact gccgcataag gcaccagata ggtggttacc gaaccgtccg
    17101 cctctttcac gctgacatca agatctgctc caccaccggc caactgcaaa tctgtaatcg
    17161 caaacgggcc aggaggaacc tctttctgat aaaccacaaa accattctgt tcaatagtta
    17221 ccagcgcgtt actctgagca atcccctgca cccgtggcgt aaaattttgt ttcgagttag
    17281 gcaacatctg catatcacga aacaaccgca caccgctgaa gcgaacagaa tcaaaaatat
    17341 cgcttgatgt gtacatatca cccacgcgaa gcgtgccgag aagttgggca aatccacgtt
    17401 ccagatacag ggtattgctt ttccacaccc ctggattgtt atttgtttta ctgaaactgg
    17461 catcagaatg cagttgccac cccagtaaat ttaacccgct gttaaaacgt acatatgtac
    17521 tcttactatt acccgacgct ttatagtcac tgtaatactg actcacataa taagaggtat
    17581 aaaacgcatt aataccccgc tcccagtttt ccggtggaac atagccactt tccagttctt
    17641 ccacccaggc ctgcgggaca ctgaaatcga gacgaaaaac cccgatatcc caggtatagc
    17701 tcccaccctg aacaagttgc tcaaatgtta aacattgctt accgctggcg aagttatcgc
    17761 tattaatgcc taaccgcttg ataacttcta ttgataaaca tgtttcttgc gggttgtctt
    17821 taacaataat ctcatatttc ccgcgccatt gcttattgac ataaatatcg atgtcatact
    17881 gccccggtaa gggttgatta tcatcaagac gaatatttgc aacctgctgg tctttcattc
    17941 cacctatcat aaaatgggta tcaaaggttt cttcagctgc ataagcttca atgccgagca
    18001 ataacgctac gattgctgat gcaagtgggg tcattctcaa cataggcagc tcctgcaatt
    18061 aaattttgtc actaatatag ttgccgtggt catcgataat ggtcagatac cagttatttg
    18121 cattattact tttgacatta acactctgac tttctaaggg ggcaatcata atagtttcat
    18181 aattgacttt aacattatta gctttgacat ccgaaatcgt cacccaatta gctgagtcat
    18241 tttttatcac caatccattg ccactgcgat ttaccagtaa ctttttaaag gtcgctttat
    18301 ttaccggagc aatacccgct ggccggtaaa acaacttaat tctgttttgc atcgcaaact
    18361 tcagtgcatt tttgccttct tgctctggac tatttggtgg aatatccaga acattcagat
    18421 aaaaaatgct ttctttatta gtgggcagtt tattcggcat aattttgatt tttacttgct
    18481 gcccggaatt tgcccctatt tttgccactg gtggcgttaa catgaaagga acctgaattt
    18541 tttctggtgg taacgacgta tcgccatcat caatccacgc ctgcagcagc gaagaacggt
    18601 ttccctggtt catcaactgc accataactt ctttattttc tgccgggtaa ataatacgcg
    18661 tcccgtagat aacaattccg gcatgtgcag gaaggaccat agaaaaaatg agtaaagata
    18721 ataatccttt catcttaatg cctcttaaat taaaaagccg ccatggggat agcggccatg
    18781 tttattacct ggagaaactt gcaattttat ttattaatcc tgcgccacac gcataaccag
    18841 agaagataac acatcacctg attgcactga gttatctgtt agtttcaggt attttgcttt
    18901 aaacggaata gcatattgac cttccgctgt tttataactc tcaatattaa aaggtgcaga
    18961 aacatccagg acatcgctgt tatcacctaa atagagatag taacccacgg tatcgttgct
    19021 actggctatc gccttatcgt tgccggttat tttgtcgttg gtaatcaacg agagttttac
    19081 accagcaact tctttcgggc agctaatttt taacatgctg ctggttacac cgactgcatc
    19141 atcataggta gaatatttag ctttaccttc aaagacagtg gttggtacag tcggcatatt
    19201 aatacttact gaatcaccac ccgcttcgaa tttacacgga gattctgcaa tatttccttt
    19261 aatggttaat gttccggtat caacgtctgc agcaaatact ccagcgctca taaaaaaaga
    19321 agaaaag
//
LOCUS       DAOFQD010000089        19221 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.089, whole genome shotgun sequence.
ACCESSION   DAOFQD010000089 DAOFQD010000000
VERSION     DAOFQD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.089"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            426..992
                     /gene="mepS"
                     /locus_tag="QBF46_003239"
     CDS             426..992
                     /gene="mepS"
                     /locus_tag="QBF46_003239"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDQ6675389.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1173..2729
                     /locus_tag="QBF46_003240"
     CDS             1173..2729
                     /locus_tag="QBF46_003240"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDQ6675390.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            2811..4625
                     /locus_tag="QBF46_003241"
     CDS             2811..4625
                     /locus_tag="QBF46_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ6675391.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            4626..5720
                     /locus_tag="QBF46_003242"
     CDS             4626..5720
                     /locus_tag="QBF46_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDQ6675392.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPLHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5720..6745
                     /locus_tag="QBF46_003243"
     CDS             5720..6745
                     /locus_tag="QBF46_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6675393.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            6747..8336
                     /gene="yejF"
                     /locus_tag="QBF46_003244"
     CDS             6747..8336
                     /gene="yejF"
                     /locus_tag="QBF46_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDQ6675394.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(8340..8684)
                     /locus_tag="QBF46_003245"
     CDS             complement(8340..8684)
                     /locus_tag="QBF46_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675395.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(9017..10207)
                     /gene="bcr"
                     /locus_tag="QBF46_003246"
     CDS             complement(9017..10207)
                     /gene="bcr"
                     /locus_tag="QBF46_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDQ6675396.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(10235..10930)
                     /gene="rsuA"
                     /locus_tag="QBF46_003247"
     CDS             complement(10235..10930)
                     /gene="rsuA"
                     /locus_tag="QBF46_003247"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDQ6675397.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11080..12840
                     /locus_tag="QBF46_003248"
     CDS             11080..12840
                     /locus_tag="QBF46_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDQ6675398.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEDRFRRAHELRG"
     gene            12965..13249
                     /gene="rplY"
                     /locus_tag="QBF46_003249"
     CDS             12965..13249
                     /gene="rplY"
                     /locus_tag="QBF46_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDQ6675399.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(13388..14395)
                     /gene="yejK"
                     /locus_tag="QBF46_003250"
     CDS             complement(13388..14395)
                     /gene="yejK"
                     /locus_tag="QBF46_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDQ6675400.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            14577..14804
                     /locus_tag="QBF46_003251"
     CDS             14577..14804
                     /locus_tag="QBF46_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDQ6675401.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            14824..16584
                     /gene="pbgA"
                     /locus_tag="QBF46_003252"
     CDS             14824..16584
                     /gene="pbgA"
                     /locus_tag="QBF46_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDQ6675402.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFAAYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            16659..16735
                     /locus_tag="QBF46_003253"
     tRNA            16659..16735
                     /locus_tag="QBF46_003253"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16693..16695,aa:Pro,seq:ggg)
     gene            complement(16836..>19221)
                     /gene="yejO"
                     /locus_tag="QBF46_003254"
     CDS             complement(16836..>19221)
                     /gene="yejO"
                     /locus_tag="QBF46_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HDQ6675403.1"
                     /translation="FGVSSNTEIKGGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTT
                     LNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGG
                     AIKASTRVMEAFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLE
                     VMDGGTVTGVDKKAGGKLIVSTNVLEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVME
                     DTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINN
                     GRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSK
                     ADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFIT
                     LTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTT
                     GGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPL
                     VFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSR
                     FSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDR
                     FANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYT
                     LSNGMRADVGNTRILRAEVGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNQVKVNDD
                     GKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT         4702 a         5011 c         4655 g         4853 t
ORIGIN      
        1 tccaaaacaa ataactcaca gtaattaaca tcatcagggt tatttttata gtgaggataa
       61 tcctgatgat gcgcaccgtg ctttcatcta tcgaacgcaa aaatcattct ctaagtaaat
      121 gaatggattg catgcgtttc actcaattgt actttaattg accaaccccg cttattaact
      181 ttctgtatca ctttttctta taaaaaatca tgtaaaaccg ctcgccaaga ccgcaccaat
      241 cgggtaatct cgaactcgtt ttgcctcggc ggtagattat cctcacagca tataattttg
      301 tgcgttagtc cacagatttg gccttaagga attgtttcaa catgcccagg taattagtct
      361 cgtgtcgctt ggcatttttt tataacgata tttgtcgtta aggacttcaa gggaaaacaa
      421 acaacatggt caaatctcaa ccgattttga gatatatctt gcgcgggatt cccgcgattg
      481 cagtagcggt tctgctttct gcatgtagtg caaataacac cgcaaagaat atgcatcctg
      541 agacacgtgc agtgggtagt gaaacatcat cactgcaagc ttctcaggat gaatttgaaa
      601 acctggttcg taatgtcgac gtaaaatcgc gaattatgga tcagtatgct gactggaaag
      661 gtgtacgtta tcgtctgggc ggcagcacta aaaaaggtat cgattgttct ggtttcgtac
      721 agcgtacatt ccgtgagcaa tttggcttag aacttccgcg ttcgacttac gaacagcagg
      781 aaatgggtaa atctgtttcc cgcagtaatt tgcgtacggg tgatttagtt ctgttccgtg
      841 ccggttcaac gggacgccat gtcggtattt atatcggcaa caatcagttt gtccatgctt
      901 ccaccagcag tggtgttatt atttccagca tgaatgaacc gtactggaag aagcgttaca
      961 acgaagcacg ccgggttctc agccgcagct aataaaccgt ttggatgcaa tcccttggct
     1021 atcctgacga gttaactgaa agcactgctt aggcagtgct tttttgtttt cattcatcag
     1081 agaaaatgat gtttccgcgt cttgatccag gctatagtcc ggtcattgtt atcttttaaa
     1141 tgttgtcgta atttcaggaa attaacggaa tcatgttcat acgcgctccc aattctggac
     1201 gtaagctcct gcttacctgc attgtagcag gcgtgatgat tgcgatactg gttagctgcc
     1261 ttcagttttt agtggcctgg cataagcacg aagtcaaata cgacacactg attatcgacg
     1321 tacaaaagta tctcgatacc tattttgccg acctgaaatc cactactgac cggcttcagc
     1381 cgctgacctt agatacctgc cagcaggcta accccgaact gaccgctcgc gcggcgttta
     1441 gcatgaatgt ccgtacgttt gtgctggtga aagataaaaa aacattctgt tcatctgcga
     1501 ccggtgagat ggacattcca ctcaatgaat tgattcctgc gctcgacatt aataaaaatg
     1561 tcgatatggc gatcttaccc ggcacgccga tggtgccgaa caaacccgca atcgtcatct
     1621 ggtatcgcaa ccctttgctg aaaaatagcg gcgtctttgc cgctctgaat ctcaacctga
     1681 cgccttctct cttttatagt tcacggcagg aagattacga tggcgtcgcc ctcattattg
     1741 gcaatactgc gctatctacc ttttcttcac gtttgatgaa cgttaatgaa ttaaccgaca
     1801 tgccggtccg tgaaactaaa attgcgggca ttcctctgac cgttcggctt tatgcggatg
     1861 actggacatg gaacgatgtg tggtacgcat ttttactggg cggcatgagt ggaactgtcg
     1921 ttggcctgct ctgctattac ctgatgagcg tacgtatgcg ccccggcaga gaaatcatga
     1981 ccgccatcaa gcgcgaacaa ttttacgtgg cgtatcaacc ggtggtggat acacaagctt
     2041 tgcgagtaac gggcctggaa gtactgctac gctggcggca tcctgtcgcg ggagaaattc
     2101 ccccggatgc cttcattaac tttgccgaat cgcaaaagat gattgtgccg ctgactcagc
     2161 acctgtttga gttaattgcc cgcgatgccg cagaattaga aaaagtactg ccggtaggcg
     2221 tcaaatttgg cattaacatt gcgccggacc atctgcacag cgaaagcttt aaagcagata
     2281 tccagaaact gctcacttcc ctgcccgcac accatttcca gattgtgctg gaaattaccg
     2341 agcgcgatat gctgaaagag caagaagcca cacaactctt cgcctggctg cactcggtcg
     2401 gcgtagaaat tgctattgat gacttcggca ccgggcacag cgcgcttatc tatcttgagc
     2461 gttttacgct cgattatctg aaaattgacc gtggatttat caacgccatc ggtacggaaa
     2521 cgatcacttc acccgtactt gacgcggtgc tgacgctggc gaaacgcctc aatatgctga
     2581 cggttgctga aggggtcgaa acgccggaac aggcgcgatg gctaagcgaa cgcggcgtta
     2641 atttcatgca aggctactgg attagtcgcc ctttaccgct ggacgatttt gtccgctggc
     2701 tgaagaaacc gtatacgccg cagtggtaag gtgtgcttac gtcccttatt attcatagtg
     2761 aaagcatgcc ggattgcggc taatgatgag taaaaggaaa tccgttgcag atgattgtgc
     2821 gcatactgct gctgtttatc gctctgttca cctttggtgt gcaggcgcag gctatcaagg
     2881 aaagctatgc ctttgccgtg ctgggcgaac cccggtacgc gtttaatttc aaccattttg
     2941 attatgtgaa ccccgccgcg ccaaaaggtg ggcagataac gttgtcagcc ctcggcacct
     3001 tcgataattt caaccgctat gcactgcgcg gcaacccggg cgcacgcacc gagcagctgt
     3061 acgacacgct atttacgact tccgatgacg aaccaggcag ttattacccg ctgattgctg
     3121 aaagcgcacg ctatgctgac gattattcct gggtggaggt cgctattaat ccgcgcgccc
     3181 gttttcatga tggttcgccc attactgccc gcgatgtaga gtttactttt caaaaattta
     3241 tgaccgaagg cgtgccgcaa tttcgtctgg tctacaaagg caccaccgtc aaagccattg
     3301 caccgttaac cgtgcgcatt gagttagcta aacccggcaa agaagatatg ctgagtctgt
     3361 tttcgctgcc ggtatttcca gaaaagtact ggaaggatca caaacttagc gacccgctcg
     3421 ccacgcctcc gcttgccagt ggtccgtacc gcgttacgtc ctggaaaatg gggcaaaata
     3481 ttgtctattc ccgcgtgaaa gattactggg cagcaaactt accggtaaac cgcggacgct
     3541 ggaatttcga caccattcgc tacgattatt acctcgatga taatgtcgcc tttgaagcgt
     3601 ttaaagcagg tgcctttgat ttgcgtatgg aaaacgacgc caaaaactgg gccacgcgtt
     3661 ataccggtaa aaatttcgat aaaaaataca tcatcaaaga tgagcaaaag aacgaatcag
     3721 cccaggatac gcgctggctg gcgtttaata tccaacgtcc ggtattcagc gatcgccggg
     3781 tgcgggaagc tatcactctc gcctttgact ttgaatggat gaacaaggcg ttgttttaca
     3841 atgcctggag tcgcacgaac agttattttc agaataccga atacgcggcc agaaattacc
     3901 ccgacgccgc ggagctggtg cttctggcac caatgaaaaa agatctaccg ccagaagtct
     3961 tcacacaaat ctaccagccg ccggtatcca aaggcgatgg ctacgatcgt gacaacctgt
     4021 taaaagccga caaacttctc aacgaagcgg gctgggtgct gaagggtcag caacgcgtta
     4081 atgccacaac gggtcagcca ctcagctttg aattattgct tcccgcaagc agcaatagtc
     4141 agtgggtatt gccgttccag cacagcctgc aacggctggg tatcaacatg gacattcgca
     4201 aggtggataa ctcgcaaatc accaaccgca tgcgcagtcg cgactatgac atgatgccgc
     4261 gcctatggcg ggcgatgccg tggcccagtt ccgatttaca gatttcctgg tcatcggaat
     4321 atatcaattc cacttataat gcccccggcg tgcaaagccc ggttatcgac tcgctgatca
     4381 accaaattat tgccgcgcag ggaaataaag aaaaattatt gccgttgggg cgagcactgg
     4441 atcgcgtatt aacgtggaat tattacatgc tgccaatgtg gtacatggcg gaagaccgtc
     4501 tcgcctggtg ggataaattc tcccaacccg ctgtacgccc tgtttacagc ctgggtatcg
     4561 atacctggtg gtatgacgtt aataaagcgg ccaaactgcc gtcagccaga caacagggag
     4621 agtagatggg cgcttacctg attcgccgtc tgttgctggt gatcccaaca ttatgggcga
     4681 ttatcaccat caactttttc atcgtgcaaa ttgcgcctgg cggtccggtc gaccaggcca
     4741 tcgccgccat tgagtttggt aatgccggag tattacccgg cgcaggcggt gaaggcgttc
     4801 gtgccagcca tgcgcaaacg ggtgtcggca atatcagcga cagtaattac cgtggcggac
     4861 gcggattaga tccagaagtg atcgctgaga tcactcatcg ctacggtttc gataagccgc
     4921 tccacgaacg ttacttcaaa atgctctggg actacatccg ctttgatttt ggcgatagcc
     4981 tgtttcgcag cgcctcggtg ctgacgctga ttaaagacag tctgccggtt tccatcaccc
     5041 tcggattatg gagcacgctg attatctatc tggtgtcgat tccgttaggc attcgcaaag
     5101 ccgtttataa cggcagccgc tttgacgtct ggagtagcgc atttatcatc atcggctacg
     5161 ccattccagc ctttttgttc gccatcctgc tgattgtctt cttcgcgggc ggcagctatt
     5221 tcgacctgtt ccctctacgc ggcctggttt ccgctaactt tgattcgctg ccgtggtatc
     5281 agaaaatcac ggattatctg tggcatatca cgctgccggt gctggcgaca gtgattggcg
     5341 gctttgcggc tctgaccatg ctgacaaaaa actcattcct tgatgaagtg cgtaagcaat
     5401 acgtggtgac cgcccgagcg aaaggggtaa gtgaaaaaaa tattctctgg aaacatgtgt
     5461 tccgcaacgc catgctgctg gtgattgccg gttttccggc gacgtttatc agcatgtttt
     5521 ttaccggctc gctgctgatt gaggtgatgt tttcactcaa tggtcttggc ttactgggct
     5581 acgaagcgac cgtctcgcgc gattatcctg taatgtttgg taccttgtat attttcaccc
     5641 tgattggcct gctgctgaat attgtcagtg atatcagcta tacgctggtt gatccgcgta
     5701 tagattttga gggacgttaa tgtcgcgact cagccccgtc aatcaggccc gttgggcgcg
     5761 ttttcgccat aaccgtcgcg gctactggtc gttatggatt ttcctcgttt tgtttggttt
     5821 gagtttgtgt tctgaactta tcgctaacga taaaccgttg ctggtgcgtt atgacggcag
     5881 ttggtatttc ccgttgttga aaaactacag cgaaagcgat tttggcggcc cgctggcaag
     5941 tcaggctgat tatcaggacc cgtggctgaa acaacggctg gaaaataacg gctgggtact
     6001 gtgggcaccg attcgctttg gtgctaccag tatcaacttt gctaccgata agcccttccc
     6061 ttctccaccc tcccggcaaa actggctggg aacggatgcc aacggcggcg atgtgctggc
     6121 acgcattctc tatggcacgc ggatctcggt tctgtttggc ctgatgctga ctctctgttc
     6181 cagcgtgatg ggcgtgctgg cgggggcgct acaaggctat tacggcggta aagtcgatct
     6241 ctggggacaa cgctttattg aagtatggtc ggggatgccg acgctgtttt tgattatttt
     6301 actttccagc gtcgtacagc ctaacttctg gtggctgctg gcaattactg tcttgtttgg
     6361 ctggatgagt ctggtcggcg tggtgcgggc ggagttttta cgtactcgta atttcgacta
     6421 cattcgtgcg gcacaggcgc ttggcgtcag cgatcgcagt atcatcctgc gtcatatgtt
     6481 gcctaatgcc atggtcgcga ccctcacctt tttaccgttt attttatgta gttcgataac
     6541 caccctgacc tcgctcgatt tcctcggctt cggtctaccg ctcggttcac cgtcactcgg
     6601 tgaactgctg ttacaaggga aaaataacct tcaggccccg tggcttggga tcaccgcctt
     6661 cttgtcggtg gcgatattat tgtctttgct gatctttatt ggtgaagccg tccgcgacgc
     6721 atttgatcct aataaggcgg tgtagcatga cgcaaactct gttagcgatt gaaaatttgt
     6781 cggtgggttt tcgccatcag caaaccgtac gtacagtagt caatgatgtt tcactacaga
     6841 ttgaggctgg cgaaacgctg gcgctggtgg gtgagtcagg ttcaggcaaa agcgttaccg
     6901 cgctgtcaat tttacgcctg ctcccttccc cgccggttga atatctctcc ggcgatattc
     6961 gttttcatgg cgaatcgctg cttcatgcca gcgatcaaac gttgcgcggt gtacgcggta
     7021 ataagatcgc catgattttt caggaaccga tggtgtcgtt aaatccattg cataccctgg
     7081 aaaaacagct ttatgaagtg ctttcactcc accgcgggat gcgtcgggaa gcggctcgtg
     7141 gcgaaattct taactgcctt gatcgcgttg gtatccgcca ggcggcaaaa cggctgacag
     7201 attatccgca tcagctctcc ggcggcgaac gccagcgggt gatgattgcg atggcgctgt
     7261 taacgcgacc ggaattatta attgccgatg aaccgaccac cgcactggac gtctctgtcc
     7321 aggcgcagat tttacagctg ttgcgcgaac tgcaaggcga gctgaatatg ggcatgctgt
     7381 ttattactca taacctcagc attgtcagaa aactggccca ccgcgtggcg gtaatgcaaa
     7441 acggtcgctg tgtcgagcaa aataacgccg ctacgctatt tgcctcaccc actcatcctt
     7501 acacacaaaa gctactcaac agtgaaccgt caggcgaccc ggtgccgttg ccagaacctg
     7561 cctcaacgtt gctggatgtt gaacagcttc aggttgcctt ccccattcgc aaagggattt
     7621 tgaagcgcat tgtggatcat aatgtggtgg tgaaaaacat cagttttacg ctacgagcgg
     7681 gtgaaacact gggtttagtg ggcgagtccg gttccgggaa aagtacgacg ggactggcgc
     7741 tgctgcgact gattaattct cagggcagca tcatctttga cggtcagcca ctgcaaaatt
     7801 taaatcgccg ccagctgtta cctattcgtc atcgcattca ggtggtattt caggatccaa
     7861 actcctcgct caacccacga ctcaacgttt tgcagattat tgaggaaggc ttacgggttc
     7921 accagccgac gctttctgcc gcacaacgcg aacaacaagt gatagccgtg atgcatgaag
     7981 tgggattaga tcctgaaaca cgccaccgtt atccggcgga gttctctggt ggtcagcgac
     8041 aacgtattgc gattgccagg gcattaattc ttaagccctc gctgatcata cttgatgaac
     8101 cgacatcatc actcgacaaa acggtacagg cgcaaatatt gacgctattg aaatcattgc
     8161 aacaaaagca tcaactggcc tatttgttta tcagccacga tttgcacgtt gtccgcgcgt
     8221 tatgtcatca ggttatcgta ctgcgacaag gggaagtagt ggaacaagga ccgtgcgcgc
     8281 gcgtgtttgc cacaccgcag caggagtata cgcgtcagct actggcgttg agctgacgct
     8341 taaaaaggat tgtagtttga aaagggttcg gcaattgcca caccaaaatt tttcaatcgg
     8401 catgttgcag caaactcgtc ctggcgattt acaaacaggc acggctcacc ttcgcactcc
     8461 agcaccgaac atggaacttc gatgtcgctt aacgcttggc ttagtttgta catcaccgac
     8521 catgcattat caccatccgg gctaagcaat ttaagcgcca ccaggctgtt gtcaccgcac
     8581 ggtccatttt gcggaatcgg ttcaaccttc gaacctgcga aaccagcaga ccagcgatag
     8641 ttctgcggca gtcgatggac gattgagagt tgtaatgaag tcacttattt tccccggaag
     8701 tacattcact tcacaatttg tttacatcaa ttttaacaca tcatcaacaa tccgtcttta
     8761 tacagatcgt aaagattgat gcctcgcctg cgtggcctct atggctctga tttaagtaat
     8821 atctgcgggc gtggtcacgt ttgcggggct atatgtaccc gtttctgcga tctaactcaa
     8881 cctttttaac tacaatgatg tgacttttta cataaattga ttttacataa aataaacata
     8941 tatcggggaa atgatagatt tgtggttgac atgcatcaac aaaggaagcc ttttaccttc
     9001 ctcgttgtgc aatagatcac cgttttttcg gccgactggc gtacagacag aagagaatgg
     9061 agctggttgc gcagaatgca attgaccaaa tcatcggcca tgcagagtta aaggtcgcaa
     9121 gagaaagcaa tgcgccaaca attgccccta tgccaaaacg gaaggttcct gccagcgaag
     9181 atgccgttcc cgccatatgg ggaaactcat caagaatgac cgccatcgca ttggatgaca
     9241 ccatcgacac gcaacccaca aacgccgcaa cgccaaccac cagcgaccag aatcccagcc
     9301 ccagcagcgc actgatgacc atccacgctg ccataataaa ttgtatccac aaccccgagc
     9361 ggaacatatt taacgcgcca atgcggcgga cgaagcggct gttaaagatg gtcatcacga
     9421 acagaaaaac aatgtttagc gcaaagtaat aaccaaagtt ttccggcgcg acgtggttaa
     9481 tttcaatata aacaaacggt ccggcgctta agaatgagaa catcccggca aagctgaaac
     9541 cactggcaag catgtagctc aggacacgtt tatggcggaa cagcgccgca aagttaccaa
     9601 tagtggtacg aatgtgaaat ggctgacgac gctccggtgg taaggtttct ttaatcagga
     9661 agaaaatcat tgccgaagcc agaatcgccg ctaatgccag gatccagaag atgtaatgcc
     9721 agctcagcca caccagcacc cagccgccaa ctatcggtgc catcagcggt gcaatggttg
     9781 tcaccagcat gacaaacgac atcatccgcg agaactcttc tttcgggtaa atatcgcgca
     9841 tcagggcgtt aatgaccacg ctggccgcag ccgcagccag cccgtggaag aaacgcatca
     9901 caatcagctg atcgatggtg tttgccaacg cacacgccac cgcggcggcg gcaaacacca
     9961 gcgtaccgcc gagcaccacc ggcttacgcc cgaagctgtc tgccatcggc ccgtagatta
    10021 actgccccaa cgcaaagccc agaatataag tactgagggt catctgcgta ctgcccgccg
    10081 gtacgccaaa ctgcgctgaa attaccggta gcgcgggcag atacatatca atcgacagcg
    10141 gcatcaacat ggccagcagg ccaaggataa aaacaatagc aaacgacgaa tgctgtcggg
    10201 tggtcacaac gggctcctga aagtcattga aaagttagac gacgctggca atttcttctt
    10261 cagttaacgg acgatattca ccgggggcta aatcagcatc cagcgtaata ccgccaatac
    10321 gttcacgatg cagctcaacc acgtggttac ccacggcggc gaacatgcgt ttcacctgat
    10381 gataacgccc ttcgctgatg gtcagacgaa cctgcgttgg ggtaatcact tccagcaccg
    10441 caggcttagt gagatctttt tcgttatgca gctgcacgcc tttagcaaat tgctctgccg
    10501 tatcgtcagc tacaggtgat tccagtgtca ccagataggt cttctcgcaa tgatggcgcg
    10561 gagaagtaat gcggtgcgac cactgaccat catcagtcat cagcaccaga ccggtggtat
    10621 caatatccaa ccgccccgcc gcatgcagtt tccacgctac cggttcatca agaaaataga
    10681 gcaccgttgg gtgatcaggg tcgtccgtgg agcaaacata gccctgaggc ttattgagca
    10741 tgaagtaacg tggaccgtgt tgctgcgcca gcgggttgcc atcgtaagcg acatcatgtt
    10801 cagggagcag tttgaacgct gcattacgga cgatttcgcc atcgacggtg acacgattgc
    10861 cgcggatttc acgcccggca atagcacggc taacgccgag ttgctgtgcg ataaatttat
    10921 caagtcgcat gtgtgtgatt ttgcctgtaa aaaacggagg tcgggtaatg acccgaaaat
    10981 ctgaactgtt gtctgcccag tatagcggtc taataacttc cctcaaggga aaaagaaccg
    11041 tggcatacta ttagcagaat aatctaccta cgcgagacca tgatttttac actccgccca
    11101 tatcagcaag aagccgtgga tgccacgctc aaccattttc gtcgtcataa aacccctgcc
    11161 gttatcgtgc tgcccaccgg cgcaggtaaa agcctggtga tagcggaact ggcacggctg
    11221 gctcgtggtc gcgtactggt gctggcacac gttaaagaac tggtggcgca aaaccatgca
    11281 aagtatcagg cgctggggct ggaagccgat atttttgccg ccgggctaaa gcgcaaagag
    11341 agccacggta aagtggtatt tggcagcgtg cagtctgtcg cccgtaatct tgatgccttt
    11401 cagggtgaat tttcgctgtt gattgtcgat gaatgtcacc gtattggtga cgatgaagag
    11461 agccagtatc agcaaatcct cactcacctg acaaaagtga atccccactt acgcctgctg
    11521 gggctgactg ccacgccttt tcgattgggc aaaggctgga tctaccagtt tcattatcac
    11581 ggcatggtac gcggcgatga gaaagccctt ttccgtgact gcatttatga gctgccgctg
    11641 cgttatatga ttaaacacgg ctatctgacg ccgccagaac gactggatat gccagtagtg
    11701 caatacgatt tcagccgctt gcaggcacag agtaatgggc tgttcagcga agccgatctc
    11761 aaccgcgagc taaaaaaaca acaacgtatt accccgcaca tcatcagcca gattatggag
    11821 tttgctgcaa cgcgcaaagg ggtgatgatt ttcgccgcca ccgttgaaca cgcaaaagag
    11881 attgtgggat tactacccgc cgaagatgct gcactgatta ctggcgacac ccccggcgct
    11941 gagcgcgatg tgttaattga agattttaaa gcccagcgtt ttcgctatct ggtcaacgtc
    12001 gcggtactga ccaccggatt tgacgccccg cacgtcgatc ttatcgccat tctgcgccct
    12061 accgaatcag tgagtcttta ccaacaaatt gtcgggcgcg gtctgcgtct cgctccgggc
    12121 aagactgatt gtttaattct tgattatgcg ggtaatcctc acgatctcta cgcgccggaa
    12181 gttggtacac cgaaaggcaa aagtgacaac gttccggtac aggttttctg ccctgcctgc
    12241 ggttttgcca acaccttttg ggggaaaacg accgccgacg ggacattgat tgaacacttt
    12301 ggtcgccgct gtcagggatg gtttgaagat gacgacggtc atcgcgaaca gtgtgacttc
    12361 cgtttccgtt ttaaaaattg cccgcaatgt aacgcagaaa atgatattgc cgctcgccgc
    12421 tgccgcgaat gtgacacggt actggttgat ccggatgata tgttaaaagc ggcgctacga
    12481 ctgaaagatg cgctggtatt acgctgtagc ggcatgtctt tgcagcatgg gcacgacgag
    12541 aaaggcgaat ggttgaaaat cacctattac gatgaagacg gcgcggatgt gagtgagcgt
    12601 ttccgtctgc aaacgcccgc ccagcgaact gctttcgagc agctttttat ccgcccgcat
    12661 acgcgcacac cgggcatccc tctgcgctgg atcaccgccg ccgatatcct cgcccagcaa
    12721 gccttactgc gacatccgga ttttgtcgtc gcccgcatga aaggtcagta ctggcaagtg
    12781 cgtgaaaaag tgttcgatta cgaagatcgt tttcgtcggg cgcacgaatt acgcggttaa
    12841 tggtagtttt gattgatgta taaggcgatt gagtatagaa tctcgcccgc ttttgcatac
    12901 gcaaagcaga tcacttacct gttgctgggt cgcctgtagc aggattaatt taagagagaa
    12961 agaaatgttt actatcaacg cagaagtacg taaagagcag ggtaagggtg cgagccgccg
    13021 cctgcgtgcc gctaacaagt tcccggcaat catctacggt ggcaaagaag caccgctggc
    13081 tatcgagctg gatcacgaca aagtcatgaa catgcaagct aaagctgaat tctacagcga
    13141 agttctgacc atcgttgttg acggtaaaga aatcaaagtt aaagctcagg acgtacagcg
    13201 tcacccgtac aaaccgaagc tgcagcacat cgacttcgtt cgcgcttaat tgctgaataa
    13261 gttgtaaaaa accccgctcc ggcggggttt tttgtatctg cagattatgc ctgattacgg
    13321 tattgctatt ttttgcaggc cagataaggc gttcacgccg catctggcat tcagaacaca
    13381 aaaccgatta attgccgcca gacgtccggc gctgcaattg gtcgcgcaaa ttcggcggcg
    13441 tgcctttaat ggtcaaggta tcggttgctg gatcccagaa gatgcgttca cctaacagca
    13501 tcgcatcaaa gttaatagtc aagccaccgc cgctaccggc aaacttcgtc aactgacgca
    13561 gtgtgctccg gtctgccggg aagctctctt ccagttcgta gcctttttca gccgcaaact
    13621 ccgtgaagct cacttcgctt acgcctgcca gctctttcga cagcgactcc agttcgatct
    13681 cttcgcctgc ctgcagctgc tcattacagt agctataaac ctgctggcgc acgttctgtc
    13741 gctctgcttt atccagttgt gcttcggcag tgaaatcatc aaccgcttgc aacagcccac
    13801 ggttttgcgc tttcgcgttt aacccttcgc tggccccgag gaaatccata aagaaatccg
    13861 ccactttgcg ccctactcgc cctttcagga aagtgagata gcgggtcgac tctggattgg
    13921 tttcccattc ggttaaatcg atacgcgcaa caatatccgc atgattgata tcaagataat
    13981 gggttgggtt gatgtcgaga ttttcgttaa cgcgcatact gctcaggttg ctcaggactg
    14041 ccaccagcag atactccacc gccagataac ggtagtggca aaacagcacg aatccaccgt
    14101 cagcaaaagg atatttcgct aattcgtcac gcaggcgacc ggttgccgcg cggctaaatg
    14161 ccagaaagtc ctcttcgccc tgacgctgta agcgcagcgt ctgcgccagt tcactctctt
    14221 cgctaaacaa gccgtaagct ttatttttgg cgctatagac ccgatgcagt tctgccacca
    14281 tctcaacgac ggtttctgtc ggttccagca atgaatcgcg caacaccagc tcaaggttct
    14341 gctcatcacg cttgataagc tggtgcagag caatctggtt gatatccaga ctcatgataa
    14401 actctccttt aagaccgggc ggtattcaac caccgccggt taagcgacgc aataaaagat
    14461 ggcggtcatt ttacagaagg cggcgtaacg agaatgattt aacgcataaa aaagcagaaa
    14521 aaaaaccgtt gctacggtaa tatgttgccc tttcatcaac taactgattt cgatttatgc
    14581 cacaaatttc ccgctacagt gatgaacagg ttgaacaatt gctcgcagag ctgctcaacg
    14641 tactggaaaa acataaggct ccgaccgacc tttccctgat ggtgttaggc aacatggtca
    14701 ctaaccttat caacaccagc attgccccgg cccaacgcca ggcaattgcc aactcttttg
    14761 cccgcgcctt acagtcctct atcaacgaag acaaagcgca ctaagggaaa cagataacag
    14821 gttatggtaa ctcatcgtca gcgctaccgt gaaaaagtct cccagatggt cagttggggg
    14881 cactggtttg cactgttcaa tattctgctt tcgctcgtca ttggcagccg ttacctgttt
    14941 atcgccgact ggccgaccac gcttgctggt cgcatttatt cctacgtaag cattatcggc
    15001 catttcagct tcctggtgtt cgccgcctac ttgctgatcc tcttcccgct gacctttatc
    15061 gtcggctccc agaggctgat gaggtttttg tccgtcattc tggcaacggc gggaatgacg
    15121 ctattactga tcgatagcga agtctttact cgtttccatc tccatcttaa tcccatcgtc
    15181 tggcaactgg ttatcaaccc agacgaaaat gagatggcgc gcgactggca gctgatgttc
    15241 atcagcgtgc cggttatttt attgcttgaa ctggtgtttg cgacgtggag ctggcaaaag
    15301 ctgcgcagcc tgacgcgtcg tcggcgcttc gcgcgtccgc tggccgcatt cttatttatc
    15361 gcctttatcg cctcgcatgt ggtgtatatc tgggccgatg ccaacttcta tcgccctatc
    15421 accatgcagc gcgctaacct gccgctttcg tacccgatga cggcgcgacg ttttcttgag
    15481 aagcatggcc tgcttgatgc gcaggagtat caacgccgtc ttattgagca aggtaatcca
    15541 gacgccgttt ccgttcagta tccgttaagc gaactgcgct atcgcgatat gggcactggg
    15601 cagaatgtgc tgttgattac tgtcgatggc ctgaactact cacgcttcga gaagcagatg
    15661 cctgcgctgg caggttttgc tgagcaaaat atttcgttca cgcgccatat gagctccggc
    15721 aacactacag acaacggcat ctttggcctg ttctatggca tctcgccgag ctatatggac
    15781 ggcattctgt cgacccgtac gcctgcggca ttaattactg cgcttaatca gcaaggctat
    15841 cagctggggt tattctcatc agatggcttt accagcccgc tgtatcgcca ggcattgttg
    15901 tcagatttct cgatgccgag cgtacgcacc caatccgacg agcagaccgc cacgcagtgg
    15961 atcaactggc tgggacgcta cgcacaagaa gataaccgct ggttctcgtg ggtctctttc
    16021 aatggtacta acattgacga cagcaatcag caggcatttg cacggaaata tagccgggcg
    16081 gcaggcaatg tcgatgacca gatcaaccgc gtgctcaatg cactgcgtga ttctggcaaa
    16141 ctggacaata cggtggtgat tatcactgcc ggtcggggta ttccactgag cgaagaggaa
    16201 gaaacctttg actggtccca cggtcatctg caggtgccat tagtgattca ctggccaggc
    16261 acgccggcgc agcgtattaa tgctctgact gatcataccg atctgatgac gacgctgatg
    16321 caacgcctgc tacatgtcag cacacctgcc agcgaatatt cgcaaggtca ggatttgttc
    16381 aaccctcaac gccgtcatta ctgggttacc gcagcggata acgatacgct ggcaattacc
    16441 accccgaaaa agacgctggt actgaacaat aacggtaaat accgcactta caacttacgt
    16501 ggtgaaagag tgaaagatga aaaaccacag ttaagtttgt tattgcaagt actgacagac
    16561 gagaagcgtt ttatcgctaa ctgattaatt ataaatcagt tagcgaaata tcttacttgc
    16621 aatcggtgtg gaaaacggta gtattaacag ccacgagtcg gcacgtagcg cagcctggta
    16681 gcgcaccgtc atggggtgtc gggggtcgga ggttcaaatc ctctcgtgcc gaccaaaaat
    16741 cccaagaaaa ccaaccgtaa aggttggttt ttttatatct gcatttaatt cgataaacag
    16801 accgcgacac atcacggcct gtttattttc tgttatcaga acgtccagac cacacccgcc
    16861 tgggtattcc acggcgattc tacccctgcg ccattgccat agctgactga caaatgaccg
    16921 cttaacgtcg gggtaaacga tgaccttatc ccagcctgat aaacgccacg ggttccagcc
    16981 acatcattat taaatttgcc atcgtcatta actttcacct ggttagaatc ggcgtattcc
    17041 tgacgcacgg cggctttcag ccagggttcc agcgtcgtac cgttttgcag gtccatgtga
    17101 tagcttaccg ccgttcccac ttcagcgcgt aatatccggg tatttcccac atcagcgcgc
    17161 atgccgtttg ataacgtgta gtcctgacca tctgtggtaa agccggtaaa ggccagatag
    17221 ggtctaacac tccacaatcc gtcaacccaa cggaacccgc tctcaacatg agcacccgcg
    17281 ccgttactat tgtaatcgcc aaacgctgtt gccccattac tcatcttgcc atggatggtg
    17341 ttggcaaaac ggtcaacttt caccacccca tcaacatagg caccgttctg atgctcccaa
    17401 ccggcataag cccccagggt atagctatcg atattacctt ttccgccgcg atcaaaacca
    17461 atatcagaat gagagtaacc aaagaacaag ccgcgaattg tactgctttc ttcacgggag
    17521 aaacggctat cgataccgag cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca
    17581 tcagtggtca cgttgttgcg ggtgttaatt gccgaactcc acatcgccgt atcgtaacta
    17641 acgcctttta cgctaccaag acgctcacgc acggtgtcca gttctgcatc aaataccagc
    17701 ggttgtgcgg ccgccatatt cagcacatca gtggttgaag gggtaatttg cgcgcgattc
    17761 tctgccagac tccagctatg gttgccatta tccagcaagg tatattcata cgtaccgata
    17821 tcaacaacgc ctccggcatt gcccaacgta aatgcagcat caccgccgcc cgttgttacc
    17881 agtgtaaggc tatctcctgc tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa
    17941 tcacctgttg cctgaccggt gacgttgagc tgatcgctct gatgattagc catatcggta
    18001 cgcatataaa aattgccgtt tcccgacagg gtattggtgg tcaacgtaat gaaattttcc
    18061 gcacttgccg tcactgatga acgagtcact ggctcatcca tcataatcac atttgcgtca
    18121 gacatcgcaa ggttggctaa gttgaggagg gtgttttggt tcgtcgtagt gatatcggca
    18181 atgatggtcc atacgctatt ttcgagcgaa acgtccgctt tgctggtatc cacggcacca
    18241 tgcgttctaa aagaagcgcc ctcagaaacc actaccttac ccaccaacat agcgggtgca
    18301 taatttattt gcggcttcat gacctcaaga atgccatcat tccctctgac tgacacgtta
    18361 accattgtgc cagcccagac attagcgcgg ccattgttga taaccatatt ttcagagatg
    18421 gctttagagg aatacgtgat acttccattc tggtctgatc gaccgagatc atataccgca
    18481 tttgcctgca ctttcgtacc agtatccttt cccagcgatt gcatagtggc atgctcatca
    18541 aggatagtat caatggcctg cgtgtcctcc ataacaatga gcccggaacc atcatccagt
    18601 tcataatttt ttgacacacc atcttttata ctaaattggc ctttactgtt tgtaccactc
    18661 acttccagca cattcgttga gacaattaat tttccgcctg cttttttatc aacgccagtt
    18721 acagtcccgc catccataac ctccagtaag ccgccactat ctacagtagt attataagcg
    18781 aagtcatttt cttcaactcg caaacttccg ccgttttcca acagcatatt gttagcaata
    18841 ccattcttga tttcgaactg accgagacgg tttgttccga atgcctccat gacccgcgtg
    18901 cttgctttaa tagcaccgcc tgctttctga tctaccgcaa tcgctaatcc cccctcttta
    18961 acctccagta atcctccctt ttcgatagcg gcaaggacgg taatcccatc tttttcaacg
    19021 attaagcgcc cgcctttaat cgtgggatca ttcgctgcgc cataaacctg tagcacacca
    19081 ttattgaggg tagtctggtt tgccgcgcca cccatttgaa caatttgata accgtcattt
    19141 aatactgagt attctgcggt accattcatt tcaatgtact gataccctcc tttaatttca
    19201 gtattactac ttacaccaaa t
//
LOCUS       DAOFQD010000090        18755 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.090, whole genome shotgun sequence.
ACCESSION   DAOFQD010000090 DAOFQD010000000
VERSION     DAOFQD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.090"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..340
                     /locus_tag="QBF46_003255"
     CDS             <1..340
                     /locus_tag="QBF46_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDQ6675404.1"
                     /translation="VTNSESALIIKMNDYGDLQINILFTSRQMIIETFICPVSSISNP
                     DEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALD
                     LAEITEEYSH"
     gene            359..1060
                     /locus_tag="QBF46_003256"
     CDS             359..1060
                     /locus_tag="QBF46_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDQ6675405.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQQAVTTST
                     VGASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGDSNKS
                     SAQDVLARLQRQQGE"
     gene            1111..1770
                     /locus_tag="QBF46_003257"
     CDS             1111..1770
                     /locus_tag="QBF46_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDQ6675406.1"
                     /translation="MSGFYQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIALGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1788..2186
                     /locus_tag="QBF46_003258"
     CDS             1788..2186
                     /locus_tag="QBF46_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDQ6675407.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2196..2834
                     /locus_tag="QBF46_003259"
     CDS             2196..2834
                     /locus_tag="QBF46_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDQ6675408.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            2837..4000
                     /locus_tag="QBF46_003260"
     CDS             2837..4000
                     /locus_tag="QBF46_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDQ6675409.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPMPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4084..5709
                     /locus_tag="QBF46_003261"
     CDS             4084..5709
                     /locus_tag="QBF46_003261"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDQ6675410.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLPEQIQNDLLLRATGGVCV"
     gene            complement(5826..6101)
                     /gene="yjfN"
                     /locus_tag="QBF46_003262"
     CDS             complement(5826..6101)
                     /gene="yjfN"
                     /locus_tag="QBF46_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDQ6675411.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6250..6579)
                     /gene="bsmA"
                     /locus_tag="QBF46_003263"
     CDS             complement(6250..6579)
                     /gene="bsmA"
                     /locus_tag="QBF46_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDQ6675412.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6761..7510
                     /gene="yjfP"
                     /locus_tag="QBF46_003264"
     CDS             6761..7510
                     /gene="yjfP"
                     /locus_tag="QBF46_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDQ6675413.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLISETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7507..8262)
                     /gene="ulaR"
                     /locus_tag="QBF46_003265"
     CDS             complement(7507..8262)
                     /gene="ulaR"
                     /locus_tag="QBF46_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDQ6675414.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8370..9434)
                     /gene="ulaG"
                     /locus_tag="QBF46_003266"
     CDS             complement(8370..9434)
                     /gene="ulaG"
                     /locus_tag="QBF46_003266"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDQ6675415.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9789..11186
                     /gene="ulaA"
                     /locus_tag="QBF46_003267"
     CDS             9789..11186
                     /gene="ulaA"
                     /locus_tag="QBF46_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDQ6675416.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11202..11507
                     /gene="ulaB"
                     /locus_tag="QBF46_003268"
     CDS             11202..11507
                     /gene="ulaB"
                     /locus_tag="QBF46_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDQ6675417.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            11517..11981
                     /gene="ulaC"
                     /locus_tag="QBF46_003269"
     CDS             11517..11981
                     /gene="ulaC"
                     /locus_tag="QBF46_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDQ6675418.1"
                     /translation="MKLRDSLAENKSIRLQAEANTWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            11995..12645
                     /gene="ulaD"
                     /locus_tag="QBF46_003270"
     CDS             11995..12645
                     /gene="ulaD"
                     /locus_tag="QBF46_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDQ6675419.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12655..13509
                     /gene="ulaE"
                     /locus_tag="QBF46_003271"
     CDS             12655..13509
                     /gene="ulaE"
                     /locus_tag="QBF46_003271"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDQ6675420.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKKLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13509..14195
                     /gene="ulaF"
                     /locus_tag="QBF46_003272"
     CDS             13509..14195
                     /gene="ulaF"
                     /locus_tag="QBF46_003272"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDQ6675421.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAGDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14324..14599)
                     /locus_tag="QBF46_003273"
     CDS             complement(14324..14599)
                     /locus_tag="QBF46_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ6675422.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14926..15321
                     /gene="rpsF"
                     /locus_tag="QBF46_003274"
     CDS             14926..15321
                     /gene="rpsF"
                     /locus_tag="QBF46_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDQ6675423.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15328..15642
                     /gene="priB"
                     /locus_tag="QBF46_003275"
     CDS             15328..15642
                     /gene="priB"
                     /locus_tag="QBF46_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDQ6675424.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15647..15874
                     /gene="rpsR"
                     /locus_tag="QBF46_003276"
     CDS             15647..15874
                     /gene="rpsR"
                     /locus_tag="QBF46_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDQ6675425.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15916..16365
                     /gene="rplI"
                     /locus_tag="QBF46_003277"
     CDS             15916..16365
                     /gene="rplI"
                     /locus_tag="QBF46_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDQ6675426.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(16436..17230)
                     /locus_tag="QBF46_003278"
     CDS             complement(16436..17230)
                     /locus_tag="QBF46_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDQ6675427.1"
                     /translation="MTLPTTIYSFPAYSPRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRHTVEIHKLDLSGWQAFNKLATRCNAYDGITMNGDNSFGWNHEAPLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYFVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(17501..>18755)
                     /locus_tag="QBF46_003279"
     CDS             complement(17501..>18755)
                     /locus_tag="QBF46_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6675428.1"
                     /translation="WHMIALVCFITVINYLDRTALGIAAPTIMETTGITKEQYSWIVS
                     AFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLAFMRALMGF
                     SEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQFAFI
                     VSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHILSQ
                     RNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCLASG
                     FVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQCLS
                     TVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALAFFD
                     IIGAIALWTLIKVKDEEPQVQLATH"
BASE COUNT         4586 a         4456 c         4937 g         4776 t
ORIGIN      
        1 tgtgacaaat agcgagagcg cattaattat taaaatgaat gattatggcg atttgcaaat
       61 caatattctt tttacttccc gccaaatgat tatcgaaacc tttatttgtc cggtgagtag
      121 tatcagcaat ccagatgaat ttaatacctt cttattaaga aatcagaaga tgatgccgtt
      181 gtcatcggta gggatctcca gcgtacaaca ggaagagtat tacattgttt tcggggcgtt
      241 atcgcttaaa tcttctcttg aagatatcct gcttgagata acctcgctgg tagataacgc
      301 attggatctg gctgaaatta cagaagaata ttcacactaa ttacaaggac gggtatttat
      361 gggaatttta aaaagtttat ttacgctggg gaaatcgttt atctcccagg cggaagagtc
      421 cattgaagaa acccagggtg tgcgcatgct ggaacagcat attcgtgacg ctaaagctga
      481 actcgataaa gccggaaaat ctcgcgttga tctgctggct cgggtgaaat taagtcacga
      541 taagctgaaa gatttacgtg agcgcaaagc cagtctggaa gcccgtgcgc tggaagcgtt
      601 aagcaagaac gttaatccgt cgttgattaa cgaagttgct gaagaaatcg cgcgccttga
      661 gaatctcatt accgctgaag agcaagtgtt gtcgaatctg gaagtctccc gtgatggcgt
      721 ggaaaaagca gttacagcaa cagcgcagcg tatcgcccag tttgagcagc aaatggaagt
      781 cgttaaagcc actgaagcca tgcagcgtgc acaacaacag gctgtaacga cctctaccgt
      841 tggcgcatct tccagcgttt cgacagcggc agaatcctta aaacgcctgc aaacgcgtca
      901 ggccgaacgt caagctcgcc tggatgctgc cgcacagttg gaaaaagtcg cagacggtcg
      961 cgatcttgat gaaaagctgg cggaagccgg aattggcgat agcaataaaa gtagcgccca
     1021 ggatgtatta gcaagactgc aacgccaaca gggcgagtaa tttttttgcc agcctcactg
     1081 caaggctggc aactatttta aggataaaat atgtctggtt tttaccagcg tctgtttggc
     1141 aaggataata agcctgctat cgctcgcggt ccgctgggac ttcatctcaa tagtggcttt
     1201 acgctcgaca cgttagcgtt tcgtttgctg gaagatgaat tgctgattgc gctgccgggt
     1261 gaggaattta cggtagctgc tgtcagccac atcgctctgg gcggcggtag tcagattttc
     1321 cgctactaca cttcaggcga tgagtttctg caaatcaata ccaccggcgg cgaagatatt
     1381 gatgacattg atgatatcaa gctctttgtc tatgaagaga gctacggtat cagtaaagag
     1441 agtcactggc gcgaggcgat caacgccaaa acgatggggg caatgacctt aaactggcag
     1501 gaaaaacgct ggcagcgatt ttttaacagc gaagaaccgg gaaatatcga acccgtctac
     1561 atgctggaaa aagtagaaaa tcaaaatcat gctaaatggg aagtccataa ttttaccatg
     1621 ggctaccagc ggcaagtgac cgaagatact tacgaatatt tgctgctaaa tggtgaggaa
     1681 tcttttaacg atctcggcga gccagagtgg ttattttcgc gcgcgttggg tgtcgatatc
     1741 ccgctgacat cacttcatat tattggttaa ttacaaggac gtttacaatg catatactgg
     1801 attcacttct tgcctttagt gcctattttt ttattggcgt ggcgatggtg attatttttc
     1861 tgtttatcta ctccaaaatt acaccgcaca acgaatggca attaatcaaa aacaataata
     1921 ccgcggcgtc actggcattc agcggtacat tgttgggtta cgtgatcccc ttatccagtg
     1981 cggcaatcaa tgcggtgagt attccagact atttcgcctg gggcgggatt gcactggtga
     2041 ttcagttact cgtttttgct ggtgtcagac tttatatgcc cgcattaagc gaaaaaatta
     2101 ttaatcacaa taccgcagca ggaatgttta tgggaaccgc cgcgctggct ggcggtattt
     2161 ttaacgcagc ttgtatgaca tggtaatgga cgatcatggc cagaaaacgc aaatcaagaa
     2221 ataacagtaa aattggtcac ggagcgatca gtcgcattgg tagaccgaat aatccttttg
     2281 aaccgcgtcg caatcgctac gcacaaaaat acttaacgtt ggcgctaatg ggcggtgccg
     2341 ctttttttgt attgaaaggt tgtggcgata gtggcgatgt cgataacgac ggcgacggaa
     2401 cgttttattc gactgtgcag gattgtattg atgacggtaa taatgccgac atttgcgcgc
     2461 gtggctggaa caacgccaaa gcagcatttt atgccgatgt tccgaagaat atgactcagc
     2521 agaattgtca gtctaagtat gaaaattgtt actacgacaa cgttgaacag agttggatcc
     2581 cggtcgtttc tggatttttg ttaagccggg ttattcgtaa agatcgcgat gagcagtttg
     2641 tttataacag cggcggttcc tcttttgctt cgcgccccgt ctggcgcaac acttctggtg
     2701 attactcctg gcgctccggt tctggcaaaa aagagtctta ctcttcaggc ggctttacca
     2761 ccagaaaagc gtcgaccgtt tcgcgcggcg gctatggtcg ttcttccagc gcccgtgggc
     2821 attggggagg ctaatcatgc tgagacacaa cgttcctgtg cggcgggatc tggaccaaat
     2881 cgccgctgac aacggtttcg actttcatat catcgacaat gaaatctatt gggacgagag
     2941 tcgggcttac cgttttactc tgcgccagat tgaagagcag atcgaaaaac cgactgcgga
     3001 actgcatcag atgtgccttg aggtggtgga tcgcgcggtt aaagacgaag agatcctgac
     3061 gcaactggcg atcccgccgt tgtactggga tgtgatcgct gaaagctggc gcgcacgcga
     3121 tccttcgctg tatggccgga tggattttgc ctggtgtggc aatgcgccgg tgaagctgct
     3181 ggagtacaac gccgatacgc cgacctcgtt gtatgagtcg gcttatttcc agtggttgtg
     3241 gctggaggat gcccggcgca gcggcattat tccgcgtgat gccgatcagt acaatgccat
     3301 tcaagaacgc ctgatttcgc gctttagtga gctttacagt cgggagccgt tttatttttg
     3361 ctgctgtcag gacaccgacg aagacaggag taccgtgctg tacttgcagg actgcgccca
     3421 gcaggcaggg caggagtcgc ggtttatcta cattgaagat ctcggtttgg gcgtcggcgg
     3481 cgtactgacc gatcttgatg ataatgtcat ccagcgtgca tttaagctgt atccgctgga
     3541 gtggatgatg cgtgacgata atggtccgct gctgcgcaag cgtcgcgagc aatgggtgga
     3601 gccgttatgg aaaagtatct tgagtaataa agggctaatg ccgctgcttt ggcgcttctt
     3661 ccctggtcat cctaaccttc ttgcatcctg gtttgagggt gaaaaaccgc agattgccgc
     3721 tggcgaaagc tatgtgcgta aaccgatcta ctcgcgcgaa ggcggaaatg tcaccatttt
     3781 cgacggccag aataacgtcg ttgaccacgc tgatggtgat tacgccgatg aaccgatgat
     3841 ctaccaggcg tttcaaccta tgccgcggtt tggcgatagt tatacgctga ttggtagctg
     3901 gattgtcgat gatgaagcgt gcggtatggg tatccgcgaa gataacacgc tgatcactaa
     3961 agacacttcg cgttttgttc cgcattacat tgctggctaa gaatgtttta gcaatctctt
     4021 tctgtcagga atccgtggca gtgaccatac taatggtgac tgccattgat ggagggagac
     4081 acagtgcact ggcaaactca cactgttttt aatcaaccta tacccttaaa taacagtaat
     4141 ttatacctgt ctgatggcgc gctctgcgaa gcggtaacgc gtgaaggtgc tggctgggat
     4201 agcgattttc tcgccagtat tggtcagcag ttaggaacgg ctgaatccct tgaactgggg
     4261 cggctggcga atgtgaatcc gcctgaatta ttgcgctacg atgcgcaagg acgccgtctg
     4321 gacgatgtgc gttttcaccc cgcctggcac ctgctgatgc aggcactatg taccaatcgg
     4381 gtgcacaatc ttgcctggga agaagacgct cgctccggcg catttgtggc gcgcgcggcg
     4441 cgttttatgt tacatgcgca ggttgaggct gggtcgttat gtccaataac gatgaccttt
     4501 gccgccacgc cattgttgtt acagatgtta cccgcgccgt ttcaggactg gaccacgccg
     4561 ctgttgagcg atcgctacga ttctcactta ttgccaggtg ggcaaaaacg cggtttgttg
     4621 attggcatgg gaatgacgga aaagcagggc ggttccgatg tcatgagcaa caccacccgc
     4681 gcagagcgtc tggaggatgg ctcttatcgg ctggtggggc ataaatggtt tttctcggtg
     4741 ccgcaaagcg atgcgcatct ggtgctggcg cagaccgcgg gcggtctgtc ctgctttttt
     4801 gtgccgcgct ttttgcctga cgggcaacgc aacgcgattc gcctcgagcg gctgaaagat
     4861 aagctgggta atcgctctaa cgccagttgt gaagtggagt ttcaggatgc cattggctgg
     4921 ttattggggc aggaagggga aggaattcgt ctgatcctga aaatgggggg gatgacgcgt
     4981 tttgattgcg ccctgggtag ccatgccatg atgcgccgtg cattttcgct ggcgatttat
     5041 catgcacatc aacgccatgt ttttggtaat ccattgatcc aacagcccct tatgcgtcat
     5101 gtcttaagtc gcatggcact tcagcttgaa gggcaaacgg cgttgctgtt tcgtcttgcg
     5161 cgagcgtggg accggcgtgc cgatgccaaa gaagccctgt gggcgcgttt atttacgcct
     5221 gcggcaaaat ttgtgatctg caaacgaggt atgccgtttg tggccgaagc gatggaggtg
     5281 ctgggcggca ttggttattg cgaggagagc gagctgccgc ggctttaccg ggagatgccg
     5341 gtaaacagta tttgggaagg ttccggcaat attatgtgcc tggatgtgtt gcgcgttctc
     5401 aataagcaag cgggcgtata cgacttattg tcggaagcat ttgtggaagt gaaagggcag
     5461 gatcgctatt ttgatcgcgc ggttcgtcgt ttacagcagc agctgcgtaa gcctgctgaa
     5521 gaactggggc gagagattac tcatcagcta ttcctgctgg gctgcggtgc gcaaatgttg
     5581 aaatatgcct ctccgccaat ggcgcaggcg tggtgtcagg tgatgttaga tacgcgcggc
     5641 ggcgtacggt tgccagagca gatccagaat gatttattgc tgcgggcgac ggggggagtg
     5701 tgtgtgtaag cgtatacgac tgatgcgacg ctggtttcga ttaactcaat gaaatatgtg
     5761 aaaattgcag gccggacaag gcgctcgcgc cgcatccggc attgttcatt tgctgtagat
     5821 gacactcacg catacaggat cgcctgtaca cgccagttat caggctgctg gtcttcgtac
     5881 atggtaataa tgcggtacca tgacgcacct tgttcatcgg cctttaacgc cacgatacgt
     5941 tccacgtcct gcggatcccc tgaaatattg ctaactgaga tctgacctat ttcattcagg
     6001 cccgtcacgc agtcagcact ggcgaattct gcagactgtg ccgtggcgct cgcaggataa
     6061 gtcactaatt gcagcgaggg tgaggcaagt aattgtttca tcgtcagctc catttttcgt
     6121 ttccccacat gttgcagggc attccatcgc ggtaataaac gccctggctt ccagccggta
     6181 atttattgtg cacaggtaaa cgcataggga cgcaaagcaa tgtaaagctg actatgtaag
     6241 ttgtctgatt tatttacggt acaaaatggc ttgtgaatac cactgtcctg tcacgatggt
     6301 ttcgtccacc atgacgacaa cgtaataatc agcttttgca gcgacagctt tcgctctgat
     6361 ttctgctaat gcgtcatccg gagaaccccg aaccatcgtg cttacgctac ctattcgctg
     6421 taacccttgc gtctggtcgc ggcgaatctc ttgcggatgg tcggttactg gcggtgctgg
     6481 ctgtggcgta ccttgcagtg cgctacaggc acttaacatc aacaccaata ataaactggc
     6541 aaaccggtaa ataacgctat tacgtttcct gctaaccata gtgtagtgcc ttattaattt
     6601 aactttgggg gaatgttccc gaattgttac ctggtacgcg attttcgaat gaaaacgtga
     6661 tgaaagcgta acccacatct caacattatg aactaatacc gccagcagaa acttgtgcta
     6721 aggatttctt catgtcatac cagacaaaaa ggagagacag atgattgaaa tagaatcacg
     6781 cgagctggca gatattcccg ttcttcatgc ttatcctgtc gggcaaaaag ataccccgtt
     6841 accgtgcgcg attttttatc acggctttac ttcgtccagt ctggtgtata gctattttgc
     6901 cgttgcgctg gcgcaggctg gtttgcgggt gatcatgccg gatgcgcccg atcacggtag
     6961 tcgttttagt ggcgacgcag cgcggcggtt aaatcaattc tggcaaatct tgctacaaag
     7021 tatgcaggaa ttcactactt tacgtgcggc aatagccgaa gaaaactggc tgcttgatga
     7081 ccgtctggca gtcggtggcg cgtcgatggg cgcgatgacg gcactgggaa ttaccgctcg
     7141 ccaccccacg gtgaaatgta ccgccagcat gatgggatcg ggctatttta catcgctcgc
     7201 ccgttcactg tttccaccgc tgatatctga aacggcagca cagcagaatg aattcaataa
     7261 cattgtcgcg ccgctggcag agtgggaagc gacaaaccac ctggaacaac ttggtgacag
     7321 acctctactg ctgtggcatg gccttgacga tgacgtcgtg cctgccgacg aatcactacg
     7381 tttgcagcag gccttaagcg agacgggacg agataaactg ctaacctgtt catggcagcc
     7441 aggcgtgcgt caccgcatta cgcctgaggc gttagatgct gccgtgacat ttttccgcca
     7501 gcatctttaa acacgcagaa tgctgacccc ttgcgcttcc agttgttgca ggatttccgg
     7561 gttagcattt ttgccggtga tcagcatatc gatttgatcg gcacggctaa aaagcattcc
     7621 cgcgcgttcg ccaatcttac tgctatcaac cagtaccacc agtttcccta ccacgctcag
     7681 catcttctgc tctgccattg ccgtcagcat atcggtttta tacaaccctt cagctgtcag
     7741 tcctttgccg ctggtaaaca tccagtgccc ggcatagaga ctgttttcgc tgccctgcgg
     7801 gcttaaggtg atggactgac ttttgttgta ctgtccgccc ataatgatca cgctgtcatg
     7861 ttcctgatcg atcaggtagt ttgccagcgg tagataatta gtgatgattt gcactggctt
     7921 gccacacatt tcccgcccaa gcagaaacgc ggtggagccg cagttgatga ctacgctttc
     7981 gcccggatta accagctgcg aggccgcttt agcaatacgt actttttcat cgtgattctg
     8041 cgcctgatgc agattcatcg gcgtccagcg tgggcgctgc tgggtaatag cttctgcgcc
     8101 attgcgcact tttttcagtt tgccgctttc gtcaagttta ttgatatcgc gtcgtgcagt
     8161 ggcaggcgaa attcccagac gctcaacgac tttctcaacg gtcacaaagc ccaattgtgc
     8221 gagcatttcc aggaggattt gatgtctttg tgcttcagtc atgagctatt ccgataaaat
     8281 ttgatttttt tagatgatat ttgaaatagc caggaaatac tacgccggat agcgcgaact
     8341 actccacgct atccggtata aacgacagat tacaggaacg acttgaacgg cagatccggt
     8401 tcaatagtga agcaatcatc gaaaccacgc ggatagtggt actcgaagtt gtctttatcc
     8461 agcggccagg taaatttgcc gcccacctgc cagataaacg gcttgaagcc atacttcaga
     8521 cgatcttttt tcatctccca cagcacgcgg atttcttgcg gatcggcctg gaagtttgac
     8581 cagatgtcgt ggtggaacgg gataaccact ttcgcattca gggcttcacc catacgcagc
     8641 atatcggcgc tggtcatttt gtcggtaata ccgcgtgggt tctcaccgta cgatcctaac
     8701 gccacatcga tctgatgttc gttaccgtgc ttcgcataat agttagagta gtgggaatcg
     8761 ccactgtgat acagggaacc gccaggggtt ttgaacaggt agtttactgc gcgatcgtcc
     8821 atgccatctg gcagtacgcc agccgctttt tgatcggcag gtagggtgat cagtgcagta
     8881 cggtcgaaag cgtcaagcgc atgaatttca atgtctttca cttttacaac atcgcccggt
     8941 ttgaccacga tgcaacgctc tttcggtacg ccccagccaa tccacaaatc aacgcaggtt
     9001 ttcggcccaa tgaacggtac gtcatcagca cagttctgca tcacggcagc agcgacgtta
     9061 acgtcgatat gatcgttgtg atcgtgagtc gccagtaccg cgtcgatctg gcgaatcgca
     9121 aacggatcaa gaacaaacgg ggtggtgcgc aggtttggct gcagtttttt cacgccagcc
     9181 atgcgctgca tctggtgacc ctgtttcatt aacgggttac cgtgactttg tttgccagtg
     9241 ccgcaccaga aatcaacgca aacgttggtg ccaccttctg atttcagcca gatcccggtg
     9301 cagcctagcc accacatcgc aaatgtgcca ggggcgacct gctcttgttc aatttcttca
     9361 ttcagccagc taccccactc cgggaaagtg ctcaggatcc aggattctcg ggtgatactt
     9421 ttcactttac tcatcgccat ttaccttcat gatagttcaa ttcgaatcaa tatgtgattg
     9481 gttttgatta atcctgacac tattttttca ggaaggcaat gaccattttt tgactttttg
     9541 ccagggaagt tgttgttgat tttgagtatg gaaagattta atggaatgtg taattcaatt
     9601 aactaaatga atttaaatga ataatcgttt cgttgtgtga atcccactct atccatgtgg
     9661 aattatttgc gggtcgcgtc acatttaatc ataaataatc ttgttgtgat tacttttgaa
     9721 aattagagtg agtgcacaac attccgggtg tgtggaatac ccggttacct cttcttcagg
     9781 agatcgttat ggagatcctc tacaacatct ttaccgtgtt ttttaaccag gtcatgacca
     9841 atgccccgtt gttgctgggt attgtgacct gtctgggcta catcctactg cgcaaaagtg
     9901 tcagcgttat tattaaaggc acgattaaaa ccataattgg tttcatgttg ttgcaggcag
     9961 ggtccggcat cctcaccagc accttcaaac cggtggtggc gaaaatgtcc gaagtctacg
    10021 gcattaacgg cgcaatttcc gatacttacg cctcgatgat ggcaaccatc gaccgcatgg
    10081 gcgatgccta tagctgggta ggttacgcgg tgttgttagc gctggcgctg aacatctgtt
    10141 acgtgctgct gcgtcgcatt accggtattc gtaccatcat gctgactgga cacatcatgt
    10201 tccagcaggc gggactgatt gccgttacgt tgtttatttt cggctattcc atgtggacga
    10261 ccattatctg cacggcgatt ctggtttcgc tctactgggg cataacctcc aacatgatgt
    10321 acaaaccgac tcaggaagtg acggacggct gcggtttctc catcggtcac cagcaacagt
    10381 ttgcatcatg gattgcctat aaagtagcgc cgttcctcgg caaaaaagag gaaagcgttg
    10441 aagacctcaa actgccaggc tggctgaata ttttccacga caacatcgtc tccacggcga
    10501 ttgtcatgac catcttcttt ggtgccattc tgctctcctt cggtatcgac accgtgcagg
    10561 cgatggcagg caaagtgaac tggacggtgt atatcctgca aaccggtttc tccttcgcgg
    10621 tggcgatctt catcatcact cagggtgtgc gcatgtttgt ggcggaactc tctgaagcgt
    10681 ttaacggtat ctctcagcgc ctgatccctg gcgcggttct ggcgattgac tgtgcggcta
    10741 tttatagctt cgcgccgaac gccgtggtct ggggctttat gtggggcacc atcggtcaac
    10801 tgattgcagt tggcatcctg gtcgcctgcg gctcttccat tctgattatc cctggcttta
    10861 tcccgatgtt cttctctaac gccaccatcg gcgtgttcgc taaccacttc ggcggctggc
    10921 gtgcggcact gaagatttgt ctggtgatgg ggatgatcga aatctttggt tgcgtctggg
    10981 cggtgaaact caccggtatg agtgcctgga tgggcatggc ggactggtcg attctggcac
    11041 caccgatgat gcagggcttc ttctccatcg gtatcgcctt tatggccgtc atcattgtca
    11101 ttgcactggc ttatatgttc ttcgctggcc gcgcgctgcg cgcagaagaa gatgcagaaa
    11161 aacaactggc agaacagtct gcttaataag gagttttgat tatgaccgta cgtattctgg
    11221 ctgtgtgtgg caacggacaa ggcagttcca tgatcatgaa aatgaaagtg gaccagtttt
    11281 taacccaatc aaacattgac cacacggtaa acagctgcgc ggttggcgaa tacaaaagcg
    11341 agttgagcgg cgcggatatc atcatcgctt ctacgcacat tgcgggcgaa atcaccgtga
    11401 ccggcaacaa atatgtggtt ggcgtgcgca acatgctctc tcctgctgac tttggcccga
    11461 aactgctgga agtgatcaaa gcgcatttcc cgcaggatgt gaagtaagga cgcgccatga
    11521 aattacgtga ttcgctggct gaaaataaat ctatccgcct gcaggccgaa gcaaatacct
    11581 ggcaggaagc ggtgaaaatc ggtgttgacc tgctggtagc ggcagatgtg gtagagccgc
    11641 gttactacca ggcgattctg gatggcgttg aacagtttgg tccttatttc gttatcgcac
    11701 cgggactggc gatgccgcac gggcgtccgg aagagggcgt taagaaaacc ggtttctctc
    11761 tggtaacgct gaaaaagccg ctggagttca accacgaaga taacgatccg gtggatatcc
    11821 tcatcaccat ggcggcggtc gatgccaaca ctcaccagga agtgggcatc atgcagatcg
    11881 ttaacctgtt tgaagatgaa gagaatttcg accgtttacg tgcctgccgt accgagcagg
    11941 aagtactgga tttaattgac cgcaccaacg cggcagctta agaaggaatt gaagatgtca
    12001 ttaccaatgt tgcaagtcgc gctggacaac cagactatgg atagcgccta cgaaaccact
    12061 cgcctgattg ccgaagaagt cgacattatc gaagtgggca ccattctgtg cgtgggcgaa
    12121 ggcgtgcgtg cggttcgtga cctgaaagcg ctttacccgc acaaaatcgt actggcagac
    12181 gccaaaattg ccgatgcggg caaaatcctt tctcgtatgt gcttcgaagc caacgctgac
    12241 tgggtgaccg tgatttgctg tgcggatatc aacaccgcca aaggtgcgct ggacgtggca
    12301 aaagagttta acggcgacgt gcagatcgag ctgaccggct actggacctg ggaacaggcg
    12361 caacagtggc gcgacgcagg cattcagcag gtggtttatc accgcagccg tgacgcacag
    12421 gccgcaggcg tggcgtgggg cgaagctgac attaccgcga tcaaacgtct ttccgatatg
    12481 ggcttcaaag taaccgttac tggtggcctg gcgctggaag atctgccgct gttcaaaggc
    12541 attccgattc atgtgtttat cgctggtcgc agcatccgtg atgccgcttc tccggttgaa
    12601 gccgcgcgtc agttcaaacg ttccatcgcc gaactgtggg gctaaggagc ggatatgttg
    12661 tccaaacaaa ttccgcttgg catctatgaa aaagcgctcc ccgccgggga gtgctggctg
    12721 gaacggctgc aactggcaaa aaaattaggc ttcgattttg tcgagatgtc ggtagatgaa
    12781 acggacgatc gcctgtcgcg cctcgactgg agccgcgagc agcgtctggc gctggtgaat
    12841 gcgattgttg aaaccggcgt gcgcgtgccc tccatgtgcc tttctgctca tcgtcgtttc
    12901 ccgctgggca gcgaagatga cgcggtacgg gcgcaggggc tggagattat gcgtaaagct
    12961 atccagttcg cccaggacgt tggcattcgc gtaatccaac tggcgggcta tgacgtttac
    13021 tatcaggaag ccaataacga aacgcgtcgt cgtttccgtg acggcctgaa agagagcgtt
    13081 gagatggcaa gccgcgcgca ggtgacgctg gcaatggaga tcatggatta tccattgatg
    13141 aactccatca gcaaggcgct gggttacgcg cactatctca acaacccatg gttccagctc
    13201 tacccggata tcggcaacct gtcggcgtgg gacaacgacg tgcagatgga attgcaggcc
    13261 ggaattgggc atatcgtcgc ggtgcatgtg aaagacacca aaccaggcgt cttcaaaaat
    13321 gtgccgtttg gcgaaggcgt agtggatttc gaacgttgct tcgaaacgct caaacagagt
    13381 ggctattgcg ggccgtacct gattgagatg tggagcgaaa cggcggaaga cccggcagca
    13441 gaagtggcga aagcgcgtga ttgggtgaaa gcgcgcatgg cgaaagcggg catggtggag
    13501 gcggcataat gcaaaagctg aaacagcagg tatttgaagc caacatggat ctgccgcgtt
    13561 acgggctggt gacctttacc tggggcaacg tcagcgccat cgaccgcgaa cgcgggctgg
    13621 tggtgatcaa gcccagcggc gtcgcctacg aaaccatgaa agcaggcgat atggtggtgg
    13681 tcgatatgag cggcaaggtg gtggaagggg agtatcgccc gtcttccgac accgcgacgc
    13741 atcttgagct ctaccgccgt tatccgtcgc ttggcggcat tgtccatacc cactccacgc
    13801 atgccaccgc atgggcgcag gcggggctgg cgatcccggc attaggcacc acgcacgctg
    13861 actacttctt tggtgatatc ccgtgtacgc gtggattaag cgaagaagag gtgcagggtg
    13921 agtatgaact gaacactggc aaagtgatta tcgaaacgct gggcaacgcc gagccgctgc
    13981 atacgccggg aattgtggtg tatcagcacg ggccgtttgc ctgggggaaa gatgcccacg
    14041 atgcggtgca taacgcggtg gtgatggaag aagtggcgaa aatggcgtgg attgcgcgca
    14101 gtataaaccc gcaactgaat cacatcgaca gctatcttat gaataaacac ttcatgcgta
    14161 agcatggtcc caacgcttat tacgggcaga agtaaaacac gcgctgcgga aatttccttc
    14221 ttcgggagat aactggtctg attccgcagc cgtttttcaa aaaaagcccc ctgcgaaggg
    14281 ggcaaagcaa actatggcaa tgtttcgttg gttatacctg gtgctagcga taaatatccg
    14341 cgctggcgtg catgttgccg ttactccccg gttcccgcat cagaatcacg tggtagtacg
    14401 ttgcgccctg cgcatctgtc tcttcattta atgcctgacg cgcttcgctt tcagtggcga
    14461 aattatgatt gatataaatc acgcctaagc tttgcacatc atccatattt ctggcctgat
    14521 ggttattaat ttcaatggct gcccatgtat ttgcacttag caaaagcaca gccagaaggg
    14581 ctaaaacacg actgaacata gatacctcct cgacggctga ctttgtgtgc tctcctctgt
    14641 gatgatcttc tgatttaatt ttaatcaatg ataaagaagt tgatggtgac catttctgat
    14701 gcagttgttc aaaaaaacac catgatgaag tgtgatgaac tccaaatcag cggtttagag
    14761 gttatttgcg aaaggggaga tttatttcgg ctctgccctt gagtttagcg gggtatgtca
    14821 gtactataac gcgtcatttt tcagccgacc tttaacacgt tccttgcctc cccgggattc
    14881 ggctgaccca gacaggaggc tgaataatcc gtaaggagca attcgatgcg tcattacgaa
    14941 atcgttttta tggtccatcc tgaccagagc gaacaggttc cgggcatgat cgagcgctac
    15001 actgctgcca tcactggtgc agaaggcaag atccaccgtc tggaagactg gggccgccgt
    15061 cagctggctt acccgatcaa caaactgcac aaagcacact acgttctgat gaacgttgaa
    15121 gctccgcagg aagtgatcga tgagctggaa actaccttcc gcttcaacga tgccgttatc
    15181 cgcagcatgg ttatgcgtac caagcacgct gttaccgaag catctccgat ggttaaagcg
    15241 aaagacgagc gccgtgagcg tcgcgatgat ttcgcaaacg aaaccgctga tgatgctgaa
    15301 gctggggatt ctgaagagta atttctgatg accaaccgtc tggtgttgtc cggcaccgtg
    15361 tgcaggactc cccttcgaaa ggtcagtcca tcaggaattc ctcactgcca gttcgtgctt
    15421 gagcatcgtt ctgtgcagga ggaagccggc tttcaccggc aggcgtggtg tcaaatgccc
    15481 gttattgtta gcggacacga aaaccaggcc attactcaca gtataacggt cggcagtcgc
    15541 ataaccgttc aggggttcat ttcatgccac aaggcaaaga acggtctgag caaaatggtt
    15601 ttgcatgccg agcagattga attgatagat tctggagact agccatatgg cacgttattt
    15661 ccgtcgtcgc aagttctgcc gtttcaccgc ggaaggcgtt caagagatcg actataaaga
    15721 tatcgctacg ctgaaaaact acatcaccga aagcggtaag attgtcccaa gccgtatcac
    15781 cggtacccgt gcaaaatacc agcgtcagct ggctcgcgct atcaaacgcg ctcgctacct
    15841 gtccctgctg ccgtacactg atcgccatca gtaatcggtc acggtccatt aatacgactt
    15901 tgagaggata aggtaatgca agttattctg cttgataaag tagcaaacct gggtagcctg
    15961 ggtgatcagg taaacgttaa agcgggctat gctcgtaact tcctggtacc gcagggtaaa
    16021 gctgttccag ctaccaagaa aaacattgaa ttcttcgaag cacgtcgcgc tgaactggaa
    16081 gctaaactgg ctgaagttct ggcagctgct aatgctcgcg ctgagaaaat caatgcactg
    16141 gaaactgtta ccatcgcgtc taaagctggc gacgaaggta aactgttcgg ttccatcggt
    16201 actcgcgaca tcgctgacgc tgtaactgca gctggcgttg aagtggctaa gagcgaagtt
    16261 cgtctgccga acggcgttct gcgtaccact ggcgaacacg aagtgagctt ccaggttcac
    16321 agcgaagtat tcgcgaaagt gatcgtaaac gtagtagctg aataattcgt tattcaacga
    16381 gacgtaaaaa cgccgaccat tggtcggcgt tttgctttct atttttcgtc aggtattagt
    16441 ttcgcaagta gatccattca ccattctctt gtgtccataa gtcaggatgg tttgctggcg
    16501 ttagatacat acgagcgcaa ctgatgtctc gctcctgcgg cactcgctcg aaacccaaac
    16561 ccgagtagat tgcggctgaa cttgataagg aacgcagacc aattttcaaa tcatggggta
    16621 aatcagaata cgcgtttagc aatgcagcaa tgatgaatcc gagggcatta gaaacgcctg
    16681 taacagtgcg aagttggtcg atataaagat cgattgttgg tagttcagat gaccagcaat
    16741 taggcatttg ggcaggaagg gttacaaagt atcctatcgt ttggccggta tagtctggag
    16801 tactataacc aggtcccaaa acaacctcgt gtattgtgta tttactgttg ggttcgaagg
    16861 attcaacatc ttgcaataaa tatttcagtt ctgcggttaa cctcgccccc gcgtaagctt
    16921 tattgtattt ctgggcgtga atattatcta atggagcttc atggttccat ccaaaagaat
    16981 tatcaccatt cattgttatt ccatcgtaag cattacacct cgtcgctaac ttattgaatg
    17041 cttgccagcc tgagagatct agtttgtgga tctcaacagt atgtctgcca tcgcaattat
    17101 ggtctcttcc acgatttgaa agtaatgtag cgcgtgcgta ttgatgaaat tttaatttta
    17161 ctggtttgtc ggtggacgaa aaacggggtg aatatgcagg aaatgaataa atagtggttg
    17221 gtaatgtcat tgtatcgttg cctgtttaat gtttttataa atatctcagc tgaatgttta
    17281 cagaataaag aatcgatatt aataaaaata agtttgtttt ctgaagtagt gaaaatagcg
    17341 gatggtgaaa gatattaata tcaaaaatcg ctcattgtgg atataacgat gcagcaggga
    17401 ggggggatat gattgacaac gcgttggtaa gcgaggttcg ataactcaag cttctttaaa
    17461 aacgccataa cctcaattat agcgttttta tttaaaataa ttaatgggta gctagctgta
    17521 cttgcggttc ttcatcttta actttgataa gcgtccacaa cgcaatagca ccaataatgt
    17581 caaagaaagc tagggcaata aagaacggcg cgaagccgat aatgtgaacg aatgccccga
    17641 tgaacaggtt gaaaatcaac tgaccgctcc acgcacaggc tcctgccata cccactgcgg
    17701 tagctacttc gtcttttttg aacagatcgc cacccagagt tgctgccaca gtagaaagac
    17761 attgatgcga gaacgcgcca atactaatca gtaatacggc aatgtaggga ttttcgacaa
    17821 tactcaccag accaatcgtc atcataatga cggctgcaat agtgaaggta attcttcgtg
    17881 agttaattaa actcacgccg cgatcgtgga agaacttcgc gacaaaacca ctggctaaac
    17941 agccgagatc gcccagcagg aaaggcagcc agacgaacat ggcaatttct ttcaggctaa
    18001 aatgcagcgt ttcgacgaag aaaatcggca cccagaagtt aatggttccc catgccgggt
    18061 ctgcgagaaa acgcgcgatg ccaatccccc agaaattacg ttggctgagg atatgcttga
    18121 ttgacgtttt ttctttctta tctgttttaa gatgctgttc ttgccctgat tcaatatagt
    18181 ggcgctcttc atcagaaagg cgtttggcat ctttcggatc tttataacaa aagaaccaaa
    18241 ataaagccgc gagcaaagca aggctacctg aaacaataaa cgcaaattgc cagctatgaa
    18301 acataatgca ccaggcaatc aacggtggag caagcatcgc gccgagtgag gtgcccatat
    18361 tgaaaacacc tgtcgccacg ccacgctctt ttgccgggaa ccatgttgat gcggttttta
    18421 cacccgccgg aattgccgac gcttcgctga aacccatcag ggcgcgcata aatgccagac
    18481 ctgaccatgt tccggttagt gcatggccca ttgtcgccag gccccaaatt gcggcacata
    18541 tcgcaaaact taacttcaga cccacggtat caataaagaa gcccattacc ggttgcccta
    18601 atgtataggc caactggaat gcactgacaa tccatgaata ttgttctttg gttattccgg
    18661 ttgtctccat aatcgttgga gccgcaatac ctaacgccgt tctgtccaga taattgatta
    18721 cagtgataaa gcatacgagg gcaatcatat gccat
//
LOCUS       DAOFQD010000091        18736 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.091, whole genome shotgun sequence.
ACCESSION   DAOFQD010000091 DAOFQD010000000
VERSION     DAOFQD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.091"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..333
                     /gene="nrdR"
                     /locus_tag="QBF46_003280"
     CDS             <1..333
                     /gene="nrdR"
                     /locus_tag="QBF46_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDQ6675429.1"
                     /translation="TFEVAELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDD
                     VEMAINHIKSQLRATGEREVPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFG
                     EEIARLED"
     gene            337..1440
                     /gene="ribD"
                     /locus_tag="QBF46_003281"
     CDS             337..1440
                     /gene="ribD"
                     /locus_tag="QBF46_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDQ6675430.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVMPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            1529..1999
                     /gene="ribE"
                     /locus_tag="QBF46_003282"
     CDS             1529..1999
                     /gene="ribE"
                     /locus_tag="QBF46_003282"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDQ6675431.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            2019..2438
                     /gene="nusB"
                     /locus_tag="QBF46_003283"
     CDS             2019..2438
                     /gene="nusB"
                     /locus_tag="QBF46_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDQ6675432.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            2516..3493
                     /gene="thiL"
                     /locus_tag="QBF46_003284"
     CDS             2516..3493
                     /gene="thiL"
                     /locus_tag="QBF46_003284"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDQ6675433.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            3471..3989
                     /gene="pgpA"
                     /locus_tag="QBF46_003285"
     CDS             3471..3989
                     /gene="pgpA"
                     /locus_tag="QBF46_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDQ6675434.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(4043..5017)
                     /gene="yajO"
                     /locus_tag="QBF46_003286"
     CDS             complement(4043..5017)
                     /gene="yajO"
                     /locus_tag="QBF46_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDQ6675435.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(5197..7059)
                     /gene="dxs"
                     /locus_tag="QBF46_003287"
     CDS             complement(5197..7059)
                     /gene="dxs"
                     /locus_tag="QBF46_003287"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDQ6675436.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(7084..7983)
                     /gene="ispA"
                     /locus_tag="QBF46_003288"
     CDS             complement(7084..7983)
                     /gene="ispA"
                     /locus_tag="QBF46_003288"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDQ6675437.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(7983..8225)
                     /gene="xseB"
                     /locus_tag="QBF46_003289"
     CDS             complement(7983..8225)
                     /gene="xseB"
                     /locus_tag="QBF46_003289"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDQ6675438.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8431..9879
                     /gene="thiI"
                     /locus_tag="QBF46_003290"
     CDS             8431..9879
                     /gene="thiI"
                     /locus_tag="QBF46_003290"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDQ6675439.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDNPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(9933..10523)
                     /gene="yajL"
                     /locus_tag="QBF46_003291"
     CDS             complement(9933..10523)
                     /gene="yajL"
                     /locus_tag="QBF46_003291"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDQ6675440.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(10486..11397)
                     /gene="panE"
                     /locus_tag="QBF46_003292"
     CDS             complement(10486..11397)
                     /gene="panE"
                     /locus_tag="QBF46_003292"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDQ6675441.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            11565..12056
                     /gene="yajQ"
                     /locus_tag="QBF46_003293"
     CDS             11565..12056
                     /gene="yajQ"
                     /locus_tag="QBF46_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDQ6675442.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILSAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(12184..13548)
                     /locus_tag="QBF46_003294"
     CDS             complement(12184..13548)
                     /locus_tag="QBF46_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6675443.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(13697..14587)
                     /gene="cyoE"
                     /locus_tag="QBF46_003295"
     CDS             complement(13697..14587)
                     /gene="cyoE"
                     /locus_tag="QBF46_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDQ6675444.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(14599..14928)
                     /locus_tag="QBF46_003296"
     CDS             complement(14599..14928)
                     /locus_tag="QBF46_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDQ6675445.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(14928..15542)
                     /locus_tag="QBF46_003297"
     CDS             complement(14928..15542)
                     /locus_tag="QBF46_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDQ6675446.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(15532..17523)
                     /gene="cyoB"
                     /locus_tag="QBF46_003298"
     CDS             complement(15532..17523)
                     /gene="cyoB"
                     /locus_tag="QBF46_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDQ6675447.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAALIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(17545..18492)
                     /gene="cyoA"
                     /locus_tag="QBF46_003299"
     CDS             complement(17545..18492)
                     /gene="cyoA"
                     /locus_tag="QBF46_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDQ6675448.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         4553 a         4933 c         4926 g         4324 t
ORIGIN      
        1 acctttgaag tggcggagct ggttatgccg cgtgttgtaa aaagcaacga cgtgcgtgaa
       61 ccgtttaatg aagagaaatt gcgtagcgga atgctgcggg cgctggaaaa acgtccggtg
      121 agttccgatg acgtcgaaat ggcaatcaat catattaaat cgcagctgcg cgccaccggt
      181 gagcgcgaag tgccgagcaa gatgattggc aatctggtga tggagcaatt gaaaaagctc
      241 gataaagtcg cctatatccg ttttgcctct gtctaccgca gtttcgaaga tatcaaagaa
      301 tttggcgaag agatcgcgcg cctggaggac taagccgtgc aggacgagta ttacatggcg
      361 cgggcgctaa agctggcgca acgaggacgt tttaccaccc accccaaccc gaatgtcggg
      421 tgcgtcattg tcaaagatgg cgaaattgtc ggtgaaggtt accaccaacg agcgggtgaa
      481 ccacatgccg aagtacacgc gttgcgtatg gcgggtgaaa aagccaaagg cgcaacggcg
      541 tatgtgacac tcgaaccctg tagccatcat ggccgtacgc caccgtgctg tgacgcactc
      601 atcgccgctg gcgtagcgcg cgtggtcgca gcgatgcagg atcctaaccc gcaggtcgct
      661 gggcgtggac tttaccgtct gcaacaggct ggcattgacg tcagccacgg gctgatgatg
      721 agtgaagccg agcaattgaa taaaggcttt ctcaagcgga tgcgcaccgg ctttccttat
      781 attcagttaa aacttggcgc atcgcttgat ggccgcacgg cgatggcgag cggcgaaagc
      841 cagtggatca cttcgcctca ggcgcggcgc gacgtacaac gacagcgcgc acaaagtcat
      901 gccattttaa ccagcagcgc cacggtgctg gcggatgatc cagccttaac agtgcgttgg
      961 tctgaactgg atgaacaaac tcaggcgctc tacccgcaac aaaatctccg ccagccagta
     1021 cgtattgtga ttgatagcca aaatcgcgtg atgccggaac atcgcattgt gcagcagccc
     1081 ggcgaaacct ggttcgcgcg tacgcaggaa gattctagtg agtggccgga aacggtgcgt
     1141 accttgctga ttccagagca taaaggtcat ctggatctgg ttgtactgat gatgcaactg
     1201 ggtaaacagc aaattaacag catctgggtg gaagcggggc caacgctcgc tggcgcattg
     1261 ctgcaggcgg gtttagtcga tgagctgatt gtctatatcg cacctaaact attaggcagc
     1321 gacgcccgcg gattatgcac gctgccaggg cttgagaaat tagccgacgc cccccaattt
     1381 aaattcaaag agatacgtca tgtaggcccg gatgtttgcc tgcatttagt gggtgcatga
     1441 tctcccggct cgaaagggaa gcagcgcacg aaatattatg ctaaaatccg cccccctgcg
     1501 gggccatact cgaacccgaa ggaataaaat gaacattatt gaagctaacg ttgctacccc
     1561 ggacgctcgc gtcgccatca ccatcgcgcg tttcaacaac tttatcaatg acagcctgct
     1621 ggaaggtgca attgacgcac tgaaacgtat cggtcaggta aaagatgaaa acattaccgt
     1681 tgtttgggtg cctggtgcct atgagctgcc gctggcggcg ggtgcactgg ctaaaaccgg
     1741 taaatacgac gcggtgattg cgctgggtac ggttattcgt ggtggcactg cccactttga
     1801 atatgtcgct ggtggtgcaa gcaacggcct ggcgcatgtt gcccaggaca gcgaaattcc
     1861 ggttgctttt ggggttctga ccactgaaag cattgaacaa gcgatcgaac gtgctggcac
     1921 caaagctggc aacaaaggtg cagaagctgc actgaccgcg cttgaaatga ttaatgtatt
     1981 gaaagccatc aaggcctgaa attagtaagg ggaaatccgt gaaacctgct gctcgtcgcc
     2041 gcgctcgtga gtgtgccgtc caggcgctct actcctggca gttgtcccag aacgacatcg
     2101 ctgatgttga ataccagttc ctggctgaac aggatgtaaa agacgttgac gtcctgtact
     2161 tccgtgagct gctggccggg gtggcgacta ataccgcata cctcgacgga ctgatgaagc
     2221 catacctgtc ccgcctgctg gaagaactgg gacaggtaga aaaagcagta ctgcgcattg
     2281 cgctgtacga actgtctaaa cgtagcgatg tgccatacaa agtggccatt aacgaagcga
     2341 tcgaactggc gaaatcgttc ggcgcagaag acagccataa gttcgtcaac ggcgtactcg
     2401 ataaagcagc acctgtgatt cgccctaaca aaaagtgata tccaggccgg tagattcacg
     2461 gaagaccgtt ccatgatcgc cggccttttc ttttttacct gctgaggcat aacgtatggc
     2521 atgtggcgag ttctccctga ttgcccgtta ttttgaccgt gtaagaagtt ctcgtcttga
     2581 tgtcgaactg ggcatcggcg acgattgcgc acttctcaat atccccgaga aacagaccct
     2641 ggcgatcagc actgatacgc tggtggcggg taaccatttc ctccctgata tcgatcctgc
     2701 tgatctggct tataaagcac tggcggtgaa cctaagcgat ctggcagcga tgggggccga
     2761 tccggcctgg ctgacgctgg cattaacctt accggacgta gacgaagcgt ggcttgagtc
     2821 cttcagcgac agtttgtttg atcttctcaa ttattacgat atgcaactca ttggcggcga
     2881 taccacgcgt gggccattat caatgacgtt gggtatccac ggctttgttc cgatgggacg
     2941 agccttaacg cgctctgggg cgaaaccggg tgactggatc tatgtgaccg gtacaccggg
     3001 cgatagcgcc gccgggctgg cgattttgca aaaccgtttg caggttgccg atgctaaaga
     3061 tgctgactac ttgatcaaac gtcatctccg tccatcgccg cgtattttac aggggcaggc
     3121 actgcgcgat ctggcaaatt cagctatcga tctctctgac ggtctgattt ccgatctcgg
     3181 gcatatcgtg aaagccagcg actgcggcgc acgtattgac ctggcattgc tgccgttttc
     3241 tgatgcgctt tctcgccatg ttgaaccgga acaggcgctg cgctgggcgc tctctggcgg
     3301 tgaagattac gagttgtgtt tcacggtgcc ggaactgaac cgtggcgcgc tggatgttgc
     3361 tctcggacac cttggcgtac cgtttacctg tatcgggcaa atgaccgccg atatcgaagg
     3421 gctttgtttt attcgtgacg gcgaacctgt cacgtttgac tggaaaggat atgaccattt
     3481 tgccacgcca taaagatgtc gcgaaaagtc gcctgaagat gagtaatccg tggcatctgc
     3541 ttgctgtcgg attcggaagt ggattaagcc cgatcgttcc tgggacgatg ggctcgctgg
     3601 cagcgattcc gttctggtat ctgatgacct ttttgccctg gcagctctac tcgctggtgg
     3661 tgatgctggg gatctgtatc ggcgtctatc tttgtcatca gaccgcaaaa gatatgggcg
     3721 tgcacgatca cggcagcatt gtctgggacg aatttattgg tatgtggatc acgctcatgg
     3781 cgctgccgac caatgactgg cagtgggttg ccgccgggtt tgtgattttc cgtattctgg
     3841 atatgtggaa accgtggccg atccgctggt ttgatcgcaa tgtgcatggc ggcatgggga
     3901 tcatgataga tgatattgtc gccggggtga tctccgctgg catcctgtac tttattggtc
     3961 atcactggcc gctgggcatt ctgtcgtagt tgtgcaccga tgcctgatgt gacgcttgtt
     4021 gcgtctcatc aggcctggac tcttatttaa atcctactac cggatgcggt ttatacggcg
     4081 tttccagttc ggcaatctgt tccggcttca aagtgatatc caccgcgttc aatagctcat
     4141 caagctgttc ttcccgcgat gtaccgataa tcggtgcggc aatgcccggt ttactcaaca
     4201 accaggccag cgcaacttgt gctcgtgttg caccgagttc ttcactgacg cccgttaacc
     4261 gctctgcgat ctgcgcatca ttttcatcgc tttctttata gagatttttc cccacctcat
     4321 cagacaccag tcgtgcggta gtttctcccc acggacgcgt cagtcgcccc cgcgccagcg
     4381 ggctccacgg aatcaccgcc acgccctcct gataacacag tggcagcatc tcgcgctctt
     4441 cttcgcgata aatcagattg tagtgatcct gcatactgac aaactgcgcc cagccgtgct
     4501 gtttttggag ttccagtgcc tgagcaaact gcgaagcgtg cattgatgac gcgccgatat
     4561 aacgcgcttt cccggctttt accacgtcgt tcagggcttc cagcgtctct tcgatcggcg
     4621 tgttgtaatc ccagcgatga atttgcagga tatcgacata atccatgccg agacgtcgca
     4681 ggctgtcgtc gatagagcgc aaaatttgtg cacgggataa tccttccggt aaatcaccaa
     4741 cgcgatggaa cactttggtg gcaacgacca cgtcttcacg acgggcgaaa tcccgcagtg
     4801 cgcgaccaac gatctcttcg ctgctaccat cggaatagct attggcggta tcaaagaaat
     4861 ttatgccgcc ttccagtgcg cgtttaatta tgggacggct gctctcttcc ggcagtgtcc
     4921 atgcgtgatt accacgatct ggctcgccaa aggtcataca gccgaggcaa agtcgggaaa
     4981 cgcgaaggtc ggtttttcct aaggggttgt attgcatgct gccactcctg ccatactcgt
     5041 catacttcaa gttgcatgtg ctgcggctgc attcgttcac cccagtcact tacttatgta
     5101 agctcctggg gcttcactcg tttgccgcct tcctgcaact cgaattattt agagtctatg
     5161 aataatttgt taagcatagc aggagtggag tagggattat gccagccagg ccttgatttt
     5221 ggcttccata ccggcggcat cgaggccgag ttcggcgcgc atttcttcct gagttccttg
     5281 cggaataaag aagtccggca gaccaatgtt cagcacgggt actggtttac gatgggccat
     5341 cagcacttcg ttcactccgc tacctgcgcc gcccataatg gcgttttctt ctacggtgac
     5401 cagcgcttca tggctggcgg ccatttccag aattaacgct tcatcaagcg gtttcacaaa
     5461 acgcatatcg accagcgtgg cgttcagcga ttcggcgact ttcgccgctt ctggcatcag
     5521 cgtaccaaag ttaaggatcg ccagtttctc accacgacgc ttcacaatgc ctttgccaat
     5581 tggtagtttt tccagcggcg tcagttccac gccgaccgcg ttgccacgcg ggtagcgcac
     5641 cgctgacggg ccatcgttat agtgatagcc ggtatagagc atctggcgac attcgttttc
     5701 atcgctcggg gtcataatga ccatttccgg tatgcagcgc aggtaagaga gatcaaaagc
     5761 accctgatgg gtttgaccgt cagcaccaac aatgcccgca cggtcgatgg cgaacaggac
     5821 cggaagcttt tgaatcgcca cgtcatgcag cacctgatca taggcgcgtt gcaggaaagt
     5881 ggagtaaatc gcgacaatgg gtttgtaccc accaatcgcc agaccggcgg caaaggtcac
     5941 cgcgtgttgc tcggcgattg ccacgtcgaa gtaacgatcc gggaatttac gtgaaaactc
     6001 gaccatgccg gaaccttcac gcatcgccgg agtaatcgcc atcagcttgt tgtctttcgc
     6061 tgccgtttcg cacaaccagt cgccaaagat ttttgaatag ctcggcaaac cgccgctact
     6121 tttcggcaaa caaccgctgg agggatcaaa tttaggcacg gcgtggaaag tgatggggtc
     6181 tttttctgcc ggttcataac cacgaccttt tttggtcatg atatgcagga actgcgggcc
     6241 tttcaggtcg cgcatgttct tcagcgtggt gataagcccc agcacatcgt gaccgtcaac
     6301 cgggccgatg tagttaaagc ccagctcttc aaacaacgtg ccaggcacta ccatgccttt
     6361 aatatgttct tcggtacgtt tgagcagctc tttaattggc ggaacgccag agaaaacttt
     6421 tttcccgcct tcgcgcagcg aagagtaaag cttaccggaa agcagctgtg ccagatggtt
     6481 attgagcgcg ccgacatttt cggaaatcga catttcattg tcgttgagga ccaccagcat
     6541 atcaggacgg atatcgcccg cgtgattcat cgcttcaaac gccatgccag cggtaatcgc
     6601 gccatcgcca atgacacaga cggtgcggcg atttttgcct tctttctcgg cagcaaccgc
     6661 aataccaatt ccggcactga tggaggttga tgaatgcccg acgcttaata cgtcatattc
     6721 gctttcgccg cgccacggga acggatgcag accgcctttc tgacggatgg tgccgatttt
     6781 gtcgcggcgt ccggtcaaaa ttttatgcgg ataagcctga tgccccacat cccaaatcaa
     6841 ttggtcaaac ggggtgttgt agacatagtg cagcgccacg gtcagttcga ccgtgcccag
     6901 cccggaggcg aagtgcccgc tggaacggct cacgctgtcg agtaaatagc ggcgcagttc
     6961 gtcgcagagt ttcggtaaac tctctttcgg caacagtcgt aactcctggg tggagtcgac
     7021 cagtgccagg gtcgggtatt tggcaatatc aaaactcatc aggggcctat taatacttat
     7081 tgtttattta ttacgctgga tgatgtagtc cgctagcgct tccagtgccg aggtatcgag
     7141 tgattgttca gccagttgtt tcagcgactg gcgggcatcg tcgatcagat cccgggcttt
     7201 cttccgggct tgctcaagac ccagaagtgc agggtaggta cttttaccaa gttgctggtc
     7261 ggcaccctgg cgttttccca acgttgcagt atctcccacc acatccagga tgtcatcctg
     7321 aacctggaag gcaaggccga tgctctctgc gtacttgtcg agtactggca gagcacgacg
     7381 ccctttatct ccggcgctta atgcaccaag gcgaacggcg gcgcgaatca atgcgccggt
     7441 tttatgacga tgaatacgct cgagtgcgtc cagaggtacg tgtttgcctt ccgcatctaa
     7501 atctaatgcc tgaccaccgc acattccggc aataccgctg gcgctcgcca gttcagaaat
     7561 catcgaaatt ctgtcgcgat ccgacacttc cggcatatcg gcatcgctta gaatcgagaa
     7621 cgccagcgtt tgtaaagcgt caccagcgag aatcgcgttt gcttcgccaa acttcacatg
     7681 gcaggtcggc aaaccgcgac gcagatcgtc atcatccatc gccggtaaat catcatgaat
     7741 taatgagtaa gcatggatac actctacggc agcagcgggt gcgtccagcg tgtttgtgct
     7801 aacgccaaac atatgaccgg tggcataaac caggaaaggt cgcaggcgct taccacctaa
     7861 taatgcgcca tactgcatgg tttcgaccac gggagtgttc tgaaagggca gtggggcgat
     7921 aaaacggctc agcgcctggt tggcctgctt aacgcaggct tcgagttgct gcggaaagtc
     7981 cattactcat tgtccggtgt aaaaggggtt agagaggcgt cttcattgtc agacagcaga
     8041 atttgcacgc gctgttcggc ttgttgtaat ttggcctgcc cctgacgtgc cagctgcacg
     8101 ccgcgttcga actcgttcag cgcctcttcc agcggcaggt cgccactttc cagacgggtt
     8161 acaatctgtt ccagctcgct cagcgccttt tcaaagctgg cgggcgcctc atttttcttc
     8221 ggcataatga atgtctgact ctcaatattt ttcgccccgt catggtaacg gactcagggc
     8281 aaatagcaaa taacgcgcaa tggtaaggtg atgtgcgcag caaagcgatg ttagtggtat
     8341 acttccgcgc ctggatgcag ccgcaggtgt gggctgctgt atttttccct atacaagtcg
     8401 cttaaggctt gccaacgaac cattgccgcc atgaagttta tcattaaatt gttcccggaa
     8461 atcaccatca aaagccaatc tgtgcgcttg cgctttataa aaatccttac cggaaacatt
     8521 cgtaacgttt taaagcacta tgatgagacg ctcgctgtcg tccgccactg ggataacatc
     8581 gaagttcgcg cgaaagatga aaaccagcgt ctggctattc gcgacgctct gacccgtatt
     8641 ccgggtatcc accatattct cgaagtcgaa gacgtgccgt ttaccgacat gcacgatatt
     8701 ttcgagaaag cgttggttca gtatcgcgat cagctggaag gcaaaacctt ctgcgtacgc
     8761 gtgaagcgcc gtggcaaaca tgattttagc tcgattgatg tggaacgtta cgtcggcggc
     8821 ggtttaaatc agcatattga atccgcgcgc gtgaagctga ccaatccgga agtgactgtc
     8881 catctggaag tggaagacga tcgtctcctg ctgattaaag gccgctacga aggtattggc
     8941 ggtttcccga tcggcaccca ggaagatgtg ctgtcgctca tttccggtgg tttcgactcc
     9001 ggtgtttcca gttatatgtt gatgcgtcgc ggctgccgcg tgcattactg cttctttaac
     9061 ctcggcggcg cggcgcatga aatcggcgta cgtcaggtgg cacattatct gtggaaccgc
     9121 tttggcagct cccaccgcgt gcgctttgtc gctattaatt tcgaaccggt cgtcggggaa
     9181 attctcgaga aaatcgacga cggtcagatg ggcgttatcc tcaaacgtat gatggtgcgt
     9241 gccgcatcta aagtggctga acgttacggc gtacaggcgc tggtcaccgg cgaagcactc
     9301 ggccaggtgt ccagccagac gctgaccaac ctgcgcctga ttgataacgt ctctgacacg
     9361 ctgatcctgc gtccgctgat ctcttacgac aaagagcaca tcatcaacct ggctcgccag
     9421 attggcaccg aagactttgc tcgcacgatg ccggaatact gcggtgtgat ctccaaaagc
     9481 ccgacggtga aagcggttaa atcgaagatt gaagcggaag aagagaagtt cgacttcagc
     9541 attctcgata aagtggttga ggaagcgaat aacgttgata tccgcgaaat cgcccagcag
     9601 accgagcagg aagtggtgga agtggaaact gtcaatggct tcggcccgaa tgacgtcatc
     9661 ctcgatatcc gttctatcga tgaacaagaa gataatccac tgaaagttga agggattgat
     9721 gtggtttctc tgccgttcta taaactgagc accaaatttg gcgatctcga ccagaacaga
     9781 acctggctac tgtggtgtga gcgcggggtg atgagccgtc tgcaggcgct ctatctgcgc
     9841 gagcagggct ttaacaatgt gaaggtatat cgcccgtaat ttgtggttgt aacgtcgcat
     9901 ctggtcagat gcgacgctta ccgcatccga cactactcgt aataattata aatccctgcc
     9961 gccatcacca gttgtgatgc cacttcatgg gctttctcgc gcccaaccag caggtcgata
    10021 attttcagac caaagtcgat agctgtaccc ggcccctggc tggtcagcaa ttttacccgt
    10081 gcatcccaga cgacgcgctt gtcctgccat tgttcagcgg gaattttgtc tttcagcgtt
    10141 gggaagccgg tcatattgcc aatcgggaag atatcgtgcg gcaccagcac ggtggctggc
    10201 gcggcgcaaa tagccgcgac gatacgcccg gaacggtgga actgtttaac ggtttcaacc
    10261 agcagggtac tatcgcgaaa acactctgcg cctttaatgc caccaggcag tacgatcacg
    10321 tcatattcgc catcagccac ctcgaccaga ggcgcatccg ccaacagctt cacgccgcgc
    10381 gagcaggtaa ttgccaggtt gccatcgctg gctacgctgg cagtggtgac tttgataccg
    10441 ccgcgaacca agaggtcgat cgtggtgacg gcttcagtct cttcactacc aggggcgagg
    10501 caaaccagtg ccgatgcgct catattcact ctcctttctt tttaccattt caaacaggcg
    10561 ggtgttttcc ggtacggcaa tcccatgcgc gcgggcgcgg cgtaagagaa aaccattgat
    10621 atagtcgatt tcggtgtggc gcagcgcgcg gatatcctgc aacatcgacg agatattttc
    10681 cgctgtggca tcaatcacct gcatcacgta atcacgcaaa tcttccgctg aagtatgatg
    10741 cccttcgcgt tcgatcactg ccgcgacttc ttcgcatatt tgcataattt cttgcggatg
    10801 atgacgtaat tcaccgttcg ggcaattcca gatggcagtc agtggattaa tcacgcagtt
    10861 gacggccagc ttgcgccaca gctcggcgcg aatattgtta tgccaggcaa cgtcaggcaa
    10921 cacggtttgc aaaatatccg ccagataact gtaatcgcca tcctgttgcc gtgccggacc
    10981 gatatgcgtg attccgtttg ccacatgaat aatgacattg ccgtcgcggc gggcggcatg
    11041 ggtggtggtg cccatcagta atggctgctg aatgttttgc aattcttcga tggtgcccat
    11101 gccgttgtga attaacagta ttggcgtagt tacaggcagt gtggacgcga ggcttttgac
    11161 ggcatcggaa acctgccatg ctttcagcgt caccaggagc agatcgctgg tggcaagaaa
    11221 atcgggatcg ttggcggtca gcgattcgtt aaatatcgaa ccatctgtct caaccagatt
    11281 cacactacaa taaggttgcg gtacgcgcag ccagccctga acttcgtgac cctgtttaca
    11341 aagtgctgta agccataatt gccctaaggc accgcatccc aatacggtaa ttttcattgt
    11401 tcctcctcac ccgcaaccac tccgggtgtt caataaggct atcccttaat tgtgcatgct
    11461 gttgcgacta tgcacaatta agggatacgt cctggtgcag gactgttggt tatttaactt
    11521 tgcgggtatt atgcttcgca tcaaaaatga agggagagga aaagatgcca tctttcgata
    11581 ttgtctctga agttgatctt caggaagcac gtaacgcggt cgataacgcg agccgcgaag
    11641 tggagtcccg ttttgacttc cgtaacgttg aagcctcatt tgagctgaat gacgccagca
    11701 aaaccatcaa agtgttgagc gagtccgatt tccaggtcaa tcagttgctg gatattttga
    11761 gtgccaagct gctgaagcgc ggcattgaag gcagttcact ggatgtaccg gaaaatatcg
    11821 ttcatagcgg taaaacctgg tttgtggaag cgaaactgaa acagggcatt gagagcgcca
    11881 ctcagaaaaa aatcgttaag atgatcaaag acagcaaact gaaagtgcag gcgcaaattc
    11941 agggcgatga gatccgcgtg actggcaaat ctcgtgatga tttgcaggct gtcatggcga
    12001 tggtgcgcgg tggcgatctg ggtcagccgt tccagttcaa aaacttccgc gattaatcgc
    12061 gacaacctgc gctttgttca tgtcagatgc agcatgaacg cctggttcgg cctacaaagg
    12121 tttgtaaatc aggagcttag taggcctgat aagtgtagcg cagcaggcaa tttcgcctcg
    12181 cttttatgcc tggcgaattg cctgttcaac atcaaagcga ttcgtcactt tgctgtcgat
    12241 tttcacataa gctgaatgtt cttcttctgc aatcaacact tctttgatgc cttcagtttc
    12301 tagcaaacgc acttttaacg cctcgtttgc ggcaatgttc gccgggattt caatgcgcaa
    12361 actgctgaca tacggcggtt ctttcatggt actggcgact gccagccaca ctgcggccag
    12421 cattgcgcca gcgagaaata cgccctgacc gtcaaacatg ccgtcaatcc agccgcccag
    12481 tgaaccgcca atcgccacgc caagaaactg gctggtggag taaacaccca tcgccgtacc
    12541 tttgtaacct gctggcgact ctttactgat aagtgagggc aggagggctt ccatcaaatt
    12601 aaacgccaca aagaaaagct gcacgccgac caccagttgc cagaactgcg tttgcgcgtt
    12661 ccacaacaca atttccgcaa ccacgatcaa cccgacgcag aagacaaaga cttgcttcat
    12721 tttgcgctta acttcagcgt agataatgaa aggcacgacc gagccaaagg cgattagcat
    12781 tgtcgccaga tagaccttcc agtgttcagc cgccgggaac cctgcatcag ccagttgtcc
    12841 gggcagggca acaaacgtcg acatcagcaa aatatgcaga cacataatgc caaagttgag
    12901 tttcagcagc cgcggttccg ccagcacttt actgaaactg cctttcacca ttccggactc
    12961 acgattaagt acgtgagtgc tactgttggg cacaacccaa atggtcaacg caatgccggt
    13021 cgttgccaga atagcgatca tccagaacag cgcgtgcagc ccaagtttgt gagtgatgat
    13081 cgggccaagc accatcgcaa tggcaaaggt aatgccaaag ctcacgccga taaacgccat
    13141 tgctttggtg cggttttgtt cgcgcgtgag atcggaaagc agcgccataa cggcagcggc
    13201 aatcgcaccg gagccttgta gcgcccgacc cagaataatt ccccagatgg agtcagagag
    13261 cgcagcgata acgctaccgg cggcaaacac tgccagccca ccgacaatta atggtttgcg
    13321 accaatgcgg tcggaaagca ggccaaacgg aatctgaaaa acggcctgag tcagaccata
    13381 aataccaatg gcaataccga ttaatgcttc gctggcacct tgcagagcca tgccgtacgt
    13441 ggtcagaacc ggcagaacca tgaacatgcc cagcatgcgc aacgagaata cggtccctaa
    13501 accccaggtc gcgcgccgct cacctggcgt cattttataa tcgttcatta ccacctctgt
    13561 tttaaattcg cgactagtgt aaagcggcaa acgaatgggg taaacatgcg gattttttag
    13621 taaatatttc gagagggata tgtttctaat gctaagaaaa aaggtgccgt agcacctttt
    13681 taatagagag gttgtgttac cacacagcag ccagcagcgt atgcgagtcc ggcaccataa
    13741 aatcaacgga catcatcacc gagagggcag tgatggcgat gatagagaag ccgaacagct
    13801 tgcgcgccca gattctgtca tcagcaactt tataaccgcg cagggccata cctaaccacc
    13861 agacgctcac cgccgcggcg accaccagat atttatatcc agcgtaaccg ccaagagaga
    13921 gcatcagcgt ggcaacggca aaggcgatga tatacagcgt gatgtgattc ttcgccactg
    13981 aaatgccttt taccactggc aataccggaa tgtttgccgc ctggtaatcc ttaaagcgga
    14041 aaatggcgat ggcataggag tgaggcatct gccacaggct gaagatagcc agcaggatcg
    14101 ctgcgccgct atcgaactcg ccggttactg cacagtagcc aatcaccggc ggcgcagcgc
    14161 cggagagcga accaatcaat gtgccgtaga ctgagtggcg tttcatgtac aggctataaa
    14221 cgccgacata aaccacaaag cccatcaccc ccagccagca ggccagcgga ttcgcgccaa
    14281 accacagcag cataaagcca gcaataccca gcaaggtggc gtacaccagc gagacagcag
    14341 gagagatcag gcctttaacc agcacccgat tcttcgtcct ttccatcttt ctgtcgatat
    14401 ccctgtcgat gtagttgtta aacacacaac ccgacgccac aaccagtgac accccaacca
    14461 gcgtgtagat aaacagggga taatcaatgc tgccctttga ggccagcagg aatcccccaa
    14521 tcaccgagat caggttgcca aagatgatgc ctggtttcgt tacttgcagg tattgcttaa
    14581 acatcataac cgccgctctt agtgcatcat catgttgtag ttgaggttcc acataatcca
    14641 gatggagcct acaaccagga tagcgatgat tagcacggtg aagacaaacg ccgtcatgtt
    14701 ccagccttca tctgatttgg tattcatgtg caggaagcac accagatgca ccagaacctg
    14761 taccactgcc attgccagga ttgttcccag aattacggcc ggagaggcag cccctgtcat
    14821 caccatccag aacggaatca ccgtcagaat gatcgacagg ataaagcctg tcatgtaggt
    14881 ttttacgctg ccatgggacg cgccgctgtg atcggtagaa tgactcatta catcgccccc
    14941 atcagataaa caacagtgaa cacacagatc caaaccacat ccaggaagtg ccagaacagg
    15001 ctcaggcaca tgatgcgggt acggttagtg ctggtcaggc cgcgacgagc gatttgtacc
    15061 atcagcaccg ccatccagat aagaccggaa gtgacgtgca gaccgtgcgt gccgaccagc
    15121 gcaaagaacg ctgacaggaa gccgctgcga tccggaccca tgccgttaac aatcaggtga
    15181 tggaattcgt agatttccat cccgataaat ccggcgccaa acaaccaggt caacgccagc
    15241 caggagataa cctggctttt gttgtttttg tacatggcga tagccgccat gccgtaggtg
    15301 atggagctga acaacagcaa gaaagtttca accagaacga acggcagttc gaaaatgtcc
    15361 ttacctgtcg ggccgcctgc ggtgccgttc accagaacgg cataggtagc aaacaagata
    15421 gagaacagaa tgcagtcgct catcaggtag atccaaaatc cgaagatttt ggttccgcct
    15481 gcatcgtggt gcccgtgttc gtgcgcgtgg acagtcgcgt gcgtcaaagt atcagttgcc
    15541 atttttcagc cctgccttag taatctcatc gaaatgctgg ttttccagtt tttcgatttc
    15601 tgccaccggc acgtagtaat ccacgtcctc gtcgaagctt ttcacgatcc aggtgatgat
    15661 catgcctgcg aagccaacaa tcgccagcca ccagatatgc cagatcatgg cgaaaccgaa
    15721 gatggtggag aaagctgcaa tgacgatacc tgcaccgctg tttttcggca tatgaatttc
    15781 ttcatagtgg tcaggctttt tatacgcttc gcctttctct ttcatttccc agaatgcatc
    15841 acgttcgtga acgtgcggca ctacggcaaa gttatagaac ggaggcgggg aagaggttgc
    15901 ccactccagc gtacggccac cccacgggtc gccagtcagg tcacggttct ggtcgcggtc
    15961 gcgaatagaa acgtacatct gaataacgag gcagagaata cccagcgcaa tcagtgctgc
    16021 accgctggct gcaatcatca gcatggtgtg gaactgcggg tcaatctgct ggctcaaacg
    16081 acgggtcatg cccatgaagc ccagcgcata cagcggcata aaggcaacga agaagccgat
    16141 gatccagaac cagaacgcgc gtttacccca ggtttcgttc agtttgaaac cgaacgcttt
    16201 aggccaccag taggtcatcc ctgcgaagca gccgaagacc acgccgccga tgatcacgtt
    16261 atggaagtgc gcaatcagga acaggctgtt atgcagaacg aagtccgcgc ccggtactgc
    16321 cagcagcacg ccagtcatac cgcccaccga gaaggtgacg ataaaaccga tagtccacag
    16381 catcgcagaa tggaacacga tgcggccctg atacatggtg aacagccagt tgaagatctt
    16441 caccccggtc gggatggcga taatcattgt ggtgatacca aagaaggcgt ttacgttcgc
    16501 gcccgcaccc atcgtaaaga agtggtgcag ccaaacgatg aacgacagca cggtgatgca
    16561 gacggttgcc cataccagcg aggtataacc aaacagacgt ttacgcgaga aggttgccgc
    16621 aatttcggag aacacaccga aaacaggcag gatcaggatg taaacttccg ggtggcccca
    16681 ggcccaaatc aggttgatgt acatcatcat gttgccaccc atatcgttgg taaagaaatg
    16741 ggtgcccaga tagcgatcca gggtcaacaa cgcgacggta accgtcagaa ttgggaagga
    16801 agcaataatc agtacgttcg cgcacagtga tgcccaggta aatactggca tcttgaacat
    16861 ggtcatgccc ggtgcgcgca tcttcagaat ggtaacgaag aagttgatac cggtaagcgt
    16921 cgtacctata ccggatagct ggagactcca tatccagtaa tcaacaccga ctcccggact
    16981 gtactctatt cccgatagcg gtggataggc cagccagccg gtctgcgcaa attcgcccac
    17041 gccgagagaa acgttaacca gaatcacacc aacaacagta aaccagaagc ttaagttgtt
    17101 gaggaacggg aacgcaacgt cacgcgcgcc gatctgcagc ggaaccacca ggttcatcag
    17161 accgataacg aaaggcatcg ccacgaagaa gatcataatc acgccgtgcg cggtgaagat
    17221 ctgatcgtag tggtgaggtg gcaggaagcc cgcttcaccc gccgaggcaa gagcctgctg
    17281 gctacgcatc ataatggcgt cagcaaaacc acgcagcaac atcacaatcg ccacgatgat
    17341 atacatgata ccgaggcgtt tatggtcgac ggaggtcagc cactctttcc acaggtaggt
    17401 ccacttaccg aagtaagtga tcaggccaac gagcgccaga cctcccaaaa taatgccagc
    17461 gatcgtaacc atgacgatag gttcatggaa cgggactgca tcaagtgata attttccgaa
    17521 catctttatt cttcctcaac ccctttaatg ggcggattcc gcgtggctca tgtccatgcc
    17581 ttccatacct tcgtgtgcac tgtgctcacc ttctggctgg gtcatgtcca tgctcttacc
    17641 gtgagccata aacttgttaa tcacatcggc aaacaagtct ggtttcacgt tggagaaata
    17701 ttccacctgg ttgtattcgc taggcgcggc cagtttttcg aacgcagcca tgtcagacat
    17761 ggtgttcggc gactgtttcg cttttgcgac ccattggtcg aattcggcgc gatccggtgt
    17821 tgcaatagct ttgaacttca tgcctgagaa gcccggcccg ctgtagctgg cggagatacc
    17881 gtcataagta ccgggttcgt tggcgatcag atgcaggcga gtctgcatac cggccatggc
    17941 ataaatctgg ctacccagac gcggaatgaa gaaggagttc atcacggagt tggaggtcac
    18001 tttgaagtac accggagtgt tcgccgggaa agcgatttca ttcacggtag caatgccctg
    18061 ttccgggtag atgaagaacc atttccagtc catggaaacc acttcgatgg taatgggctt
    18121 ctcgtcgtgt gccagcggct tgctaggctc aagagcgtga gtggttttcc aggtcagtac
    18181 cgcaaggaag atgatgatta agataggtac cgtccagacc acagcttcca ctttattgga
    18241 gtgtgaccag ttcgggctgt acttagcatc tttattgctc gcacgatact tccaggcgaa
    18301 accaacagcc atcaagattg cgggaataac gacaatcaac atcaggccaa atgccgtcag
    18361 tatcagtgaa cgttgctcca gaccaatctg tcctttggga tctaacagcg cagaattaca
    18421 gccactgagc aatacagtgc ctgcaaataa tgacaaccat cccaaacttt tattgtattt
    18481 cctgagtctc atttaacgac ctcaattcca cgggatctgg tggcgtttaa agtgtgtggg
    18541 cattttacgg gaaggttaca ttactgtaaa catgattaaa ttcctgttac ttagatttgg
    18601 ctgtcaggtc acatatgttg caaaacatat caagttgatt ttaagaagct gttgcaaaag
    18661 ggcgttataa cgaaagggga gtgcagaagg agtttggacg ggcaattggt ataaccaatc
    18721 tggaataaaa caattg
//
LOCUS       DAOFQD010000092        18625 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.092, whole genome shotgun sequence.
ACCESSION   DAOFQD010000092 DAOFQD010000000
VERSION     DAOFQD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.092"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..580)
                     /gene="rsmG"
                     /locus_tag="QBF46_003300"
     CDS             complement(<1..580)
                     /gene="rsmG"
                     /locus_tag="QBF46_003300"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDQ6675449.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPAL"
     gene            complement(644..2533)
                     /gene="mnmG"
                     /locus_tag="QBF46_003301"
     CDS             complement(644..2533)
                     /gene="mnmG"
                     /locus_tag="QBF46_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDQ6675450.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2912..3355)
                     /gene="mioC"
                     /locus_tag="QBF46_003302"
     CDS             complement(2912..3355)
                     /gene="mioC"
                     /locus_tag="QBF46_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDQ6675451.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     PASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWVNLLK"
     gene            complement(3445..3903)
                     /gene="asnC"
                     /locus_tag="QBF46_003303"
     CDS             complement(3445..3903)
                     /gene="asnC"
                     /locus_tag="QBF46_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDQ6675452.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4055..5047
                     /gene="asnA"
                     /locus_tag="QBF46_003304"
     CDS             4055..5047
                     /gene="asnA"
                     /locus_tag="QBF46_003304"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDQ6675453.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5052..6503)
                     /gene="viaA"
                     /locus_tag="QBF46_003305"
     CDS             complement(5052..6503)
                     /gene="viaA"
                     /locus_tag="QBF46_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDQ6675454.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6497..7993)
                     /gene="ravA"
                     /locus_tag="QBF46_003306"
     CDS             complement(6497..7993)
                     /gene="ravA"
                     /locus_tag="QBF46_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDQ6675455.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8216..10084
                     /gene="kup"
                     /locus_tag="QBF46_003307"
     CDS             8216..10084
                     /gene="kup"
                     /locus_tag="QBF46_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDQ6675456.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10251..10670
                     /gene="rbsD"
                     /locus_tag="QBF46_003308"
     CDS             10251..10670
                     /gene="rbsD"
                     /locus_tag="QBF46_003308"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDQ6675457.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10678..12183
                     /gene="rbsA"
                     /locus_tag="QBF46_003309"
     CDS             10678..12183
                     /gene="rbsA"
                     /locus_tag="QBF46_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDQ6675458.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12188..13153
                     /gene="rbsC"
                     /locus_tag="QBF46_003310"
     CDS             12188..13153
                     /gene="rbsC"
                     /locus_tag="QBF46_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDQ6675459.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13178..14068
                     /gene="rbsB"
                     /locus_tag="QBF46_003311"
     CDS             13178..14068
                     /gene="rbsB"
                     /locus_tag="QBF46_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDQ6675460.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14194..15123
                     /gene="rbsK"
                     /locus_tag="QBF46_003312"
     CDS             14194..15123
                     /gene="rbsK"
                     /locus_tag="QBF46_003312"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDQ6675461.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15127..16119
                     /gene="rbsR"
                     /locus_tag="QBF46_003313"
     CDS             15127..16119
                     /gene="rbsR"
                     /locus_tag="QBF46_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDQ6675462.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16085..17512)
                     /gene="mdtD"
                     /locus_tag="QBF46_003314"
     CDS             complement(16085..17512)
                     /gene="mdtD"
                     /locus_tag="QBF46_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDQ6675463.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLYLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17535..18227)
                     /locus_tag="QBF46_003315"
     CDS             complement(17535..18227)
                     /locus_tag="QBF46_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDQ6675464.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4490 a         4619 c         4872 g         4644 t
ORIGIN      
        1 ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg ggcaacaaag
       61 cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc tcaccaggaa
      121 gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta attacgccat
      181 caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca atattctcca
      241 gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg ctatccaaca
      301 gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt cctggtccgg
      361 tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg ctatcgagaa
      421 tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac gctttgttcc
      481 atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg gtaagcgaaa
      541 tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc tgttcttgat
      601 gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg ctacgacgca
      661 gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc gtgacgccag
      721 aaatacgcga agcttggccg atagaggctg gtttgtgatc gttaagtttg gcgatcactt
      781 cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg gtgttctcgt
      841 tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg tatttaacct
      901 gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac ggcgtcagcg
      961 tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca ctggcttcac
     1021 gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc gacggggtta
     1081 cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc tcgttaaagc
     1141 gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc aaacgcagat
     1201 ccgcattatc ttcacgtagc atcagacgat attctgcgcg cgaagtaaac atacgatacg
     1261 gttctttggt tcctaaagtg cacaggtcat caactagtac gccgagatac gcctgagaac
     1321 gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga ccggccagca
     1381 aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca gcaaagaaca
     1441 gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg aagaagtcat
     1501 actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc atagagcgga
     1561 cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga taaatttcat
     1621 tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtcg gcgaagcgca
     1681 tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc acccctgcgt
     1741 acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg ttggtatgag
     1801 tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac gagaataccg
     1861 gcattgggtt atcgccatgc tgttgcgcca gtacgctaaa gtcgatggtt cgagcatcaa
     1921 tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca cgcaaacggc
     1981 gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtaatta tccagaccga
     2041 tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggcttta gcacggaact
     2101 tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata agatcttcaa
     2161 ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt accgcctgac
     2221 ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct ttgcttgcgt
     2281 ttagtatcct aaactggata cccgcctgat cgatcgcttt cgccatcaga ccgccgagtg
     2341 catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg ttgcagctca
     2401 tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc atacgcgccg
     2461 cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gataatgacg tcaaaaggat
     2521 ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt gaagcccggg
     2581 ccgtggattc tactcaactt tgtcggcttg agaaagaccc gggatcctgg gtattaaaaa
     2641 gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat cgcactgccc
     2701 tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca ttaactgtga
     2761 atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat acacaactca
     2821 aaaactgaac aaccgttgtt ctttggataa ctaccggttg atccaagctt cctgacagag
     2881 ttatccacag tggatcgcac gatctgtata cttatttgag taaattaacc cacgatccca
     2941 gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc ttcagtgttt
     3001 cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg atagccccac
     3061 aaaaggtgtc atattcacga ctgccaatac cgattgcgcc aaagcggact gcagaaagat
     3121 cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga atatctccgg
     3181 caccgtgggt ggagctgata accagccaga tccctgaggc aggtaaatct tctaacagcg
     3241 gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc aggtgttctg
     3301 ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct gccataaacc
     3361 gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc tacctgtgga
     3421 aaagtatggg attaaaaaag ccgatcaggg cttgatggta cgcatgatcg ggttttgcag
     3481 gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt tgataagtac
     3541 atgctggaga gcgtcgatcg aacggcacat cacttttata aagatgctgt agtggccggt
     3601 tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg cggaagggta
     3661 gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac cgagctgctt
     3721 cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt tctctactcg
     3781 aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt aagcggtgcg
     3841 cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca gataattttc
     3901 cataggattt tcttatgcgg attgatgatt cattctattt tagccttctt ttttaatgaa
     3961 tcaaaagtga gttaggcttt ttattgaatg attattgcat gtgtgtcggt ttttgttgct
     4021 taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca ttgccaaaca
     4081 acgtcaaatt agcttcgtga aatctcactt ttctcgtcaa ctggaagaac gtctggggct
     4141 gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg ataacttgtc
     4201 gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc agttcgaagt
     4261 ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact tcagcgcggg
     4321 cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc tttctccgtt
     4381 gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg gtgagcgtca
     4441 attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag caaccgaagc
     4501 tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc acttcgtaca
     4561 cagccaggag ttactgtctc gttatccgga tcttgatgcc aaagggcgtg agcgggcgat
     4621 agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga gcgatggtca
     4681 tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag agctgggcca
     4741 tgcgggtctg aacggcgata ttctggtgtg gaacccggta ctggaagatg cgtttgagct
     4801 ttcctccatg gggatccgtg tagatgccga cacgctgaag catcaactgg cgctgaccgg
     4861 tgacgaagat cgcctggagc tggagtggca tcaggcgctg ctgcgcggtg aaatgccgca
     4921 gaccatcggc ggcggtatcg gccagtctcg tttgactatg ctgctgctgc aactgccgca
     4981 tatcggccag gttcagtgtg gagtatggcc agctgctgtt cgcgagagcg tcccttctct
     5041 gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc ggtatcaaag
     5101 cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga catcgccacg
     5161 gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt cacgtcgtca
     5221 ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa ccattccctg
     5281 ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc gccacgaaac
     5341 tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag ctcataacgg
     5401 acgatctcgg tggaaaatag cataatatag cagcgccggt tttctgcgag agcaatgcgc
     5461 atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat tgagccggaa
     5521 gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt atgtaccacc
     5581 ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt gagcaactgt
     5641 ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag tgtcgccagt
     5701 tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac ctgctcagga
     5761 accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc gttgcgcggt
     5821 attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag ttccggctgt
     5881 tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt aagctgaccg
     5941 gcgctcatat cccacagacg accagctgca gtattgttat ctgcgagaat cggttcaagt
     6001 tgtccgctca gcgtcatgcg ttcctgaact tcactcaaca gttgttcgcg ttcttcttct
     6061 aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca acgctggaga
     6121 aaaagcgtgt gtaacgctga agtgatcgtg ctgttagcat caaccaactg tcgggcttgt
     6181 tctgcccatg gagaattcag acgatgcagt aagtccagga tctgtggtag ctgcacaata
     6241 aactgtggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc ggtgagttcc
     6301 ggcgggactc gggcatcttt cagccgactg cgcagcgctt cacgccagcg gggaacatca
     6361 tcagtgattg ccgccttcag gcgtgggaat ttttcaaaga agaccgccag ctgcggtgag
     6421 gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc cagcatcaca
     6481 ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg acatcctgta
     6541 ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt tgctgttcgc
     6601 tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccagttgt tgttttattt
     6661 caccaggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct tgtaaactta
     6721 catcgcgaac gacaagatgt tgggcgctat caacttccag attcagtttc tgggcaaagc
     6781 cgataccgtt cagtttaccg cgaatttcac cacctttgct cagccactgt tccagcgcgc
     6841 tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt ttttgcagca
     6901 gtagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga cgacggctga
     6961 aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc tgctggagtt
     7021 gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt tgccaggcgt
     7081 gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc gcgtcatacc
     7141 acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga ccgctaaaaa
     7201 aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag acataaggcg
     7261 catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat acatgatcgg
     7321 gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct gtgacctgca
     7381 aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg gagcggaaat
     7441 tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca tataacgctt
     7501 ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga cgcatcggga
     7561 ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg agcaaggtat
     7621 taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct gcttccggca
     7681 ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga atagaaaggg
     7741 gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac gcgcgggcat
     7801 tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct ggcgggccaa
     7861 ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg gcgtggctac
     7921 gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc gctaataaat
     7981 gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa attgcgagta
     8041 tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gggtattttt ctttgagcca
     8101 ggttaatgtg gccgcattta ggagtacgat tttgccgtta atcgtgcata ctgtgcgctt
     8161 ttttgtgggc caagggacta agcacacatt tcatatttca acgaaagact agtctatgag
     8221 cactgataat aagcaatcat tgcccgcgat taccctcgcg gcgattggag ttgtctacgg
     8281 cgatattggt accagcccgt tatatacact tcgtgaatgt ttgtccggcc agtttggttt
     8341 tggcgttgaa cgcgatgccg tgtttggctt tttatcgctg atcttctggc tgctaatctt
     8401 tgtggtttcc attaaatatc tcaccttcgt gatgcgggca gataacgccg gtgagggggg
     8461 gatcctgacg ttgatgtcgc ttgccgggcg taatacgtcg gcgcgaacca catcaatgct
     8521 ggtgattatg gggctaatcg gcggcagctt tttctatggt gaagtcgtca taacacccgc
     8581 tatttcggtg atgtcagcca ttgaaggtct ggaaatcgtc gccccgcagc tggatacctg
     8641 gatagttccc ctctcaatta tcgttctcac tttattattt atgattcaaa aacatggcac
     8701 ggctatggtc ggtaagctgt ttgcgccgat catgctgacc tggtttttga ttctggcagg
     8761 actggggtta cgtagcatta ttgctaaccc ggaagtgctg catgcgctga atccaatgtg
     8821 ggcggtgcat ttcttccttg aatacaaaac ggtttctttt attgcattag gggcagtggt
     8881 gttgtcgatt acgggggtcg aggcgctgta tgctgatatg gggcactttg gtaagttccc
     8941 tattcgcctg gcgtggttca ccgtcgtatt accttccttg acccttaatt acttcggtca
     9001 gggagcgctg ttgttaaaga acccggaagc gattaagaac ccgttcttcc tgttggcacc
     9061 ggactgggcg ctgatcccgc tgctgatcat cgccgcactg gcgacggtaa ttgcctcgca
     9121 ggcggttatc tctggcgtct tctcattgac gcgtcaggcg gtacgtctgg gatatttgtc
     9181 gccgatgcgc attattcaca cctccgaaat ggagtcaggg caaatctata ttccgtttgt
     9241 gaactggatg ctctatgtcg cggtcgtgat tgtgattgtc agctttgagc actccagcaa
     9301 cctggcggcg gcgtacggga ttgcggtgac cggaaccatg gtgctgacgt ctattctctc
     9361 gactaccgtg gcacgtcaga actggcactg gaataagtat tttgttgcgc tgatcctgat
     9421 tgctttcctt tgtgtcgata ttccattgtt caccgctaac ctcgataaac tgctctccgg
     9481 cggctggttg ccattgagcc tcggtactgt gatgtttatc gtgatgacca cctggaagag
     9541 cgagcgtttc cgcttgctgc ggcggatgca tgaacatggt aactctctgg aagcgatgat
     9601 tgcttcgctg gagaaatcac cgcctgttcg cgtgcccggg accgcggtgt atatgtcgcg
     9661 tgcaatcaac gtcattccct ttgcgctgat gcataacctt aaacataaca aggtattgca
     9721 tgagcgggtg attctgttaa ctctgcgtac cgaagacgcg ccatatgtcc ataatgtccg
     9781 tcgggtacag attgaacaac tgtcgcccac tttctggcgc gtggtggcaa gttatggttg
     9841 gcgagaaacg ccaaacgtgg aagaagtttt ccaccgctgc ggtctggaag gattaagttg
     9901 ccggatgatg gaaacctcct tctttatgtc gcatgagtcg ttgatcctcg gcaaacgccc
     9961 gtggtatttg cgtctgcgcg gcaagctgta cttgctgctg caacgtaatg cgctgcgtgc
    10021 accagatcaa tttgaaattc cgccaaacag ggttatcgaa ctgggtactc aggtcgaaat
    10081 ctaacgccag acgcctcctt tctccataag ggggcgtttt tgttttcatg gttaatcacc
    10141 atgtaaaacg tttcgaggtt gatcacattt ccgtaacgtc acgatggttt tcccaactca
    10201 gtcaggatta aactgcgggt cagcgaaacg tttcgctgat ggagaaaaaa atgaaaaaag
    10261 gcaccgttct taattctgat atttcatcgg tgatctcccg tctgggacat accgatacgc
    10321 tggtggtgtg tgatgctggt ttacccatcc ccaaaagtac aacgcgtatc gatatggcat
    10381 taacccaggg tgtaccttct tttatgcagg tgctgggcgt cgtcacaaat gaaatgcagg
    10441 tcgaggctgc cattatcgcg gaagagatca aacaacataa tccgcaactc cacgaaacgt
    10501 tgctcactca ccttgagcag ctgcaaaaac accagggaaa taccattgaa attcgttaca
    10561 ccacgcatga acaattcaaa caacaaaccg cagaaagtca ggcggtaatt cgcagcggag
    10621 aatgttctcc gtatgcgaat atcattctct gtgctggcgt gacgttctga ggccgtcatg
    10681 gaagcattac ttcagcttaa aggcatcgat aaagccttcc cgggcgtaaa agccctctcg
    10741 ggcgcagcgt taaatgtcta tccgggccgc gtgatggcgc tggtgggcga aaacggcgcg
    10801 ggtaaatcca ccatgatgaa agtgcttact ggtatctatg ctcgcgatgc cggcacgctt
    10861 ttatggctgg ggaaagaaac gacatttacc gggccgaaat cttcccagga agccgggatt
    10921 gggattatcc atcaggaact gaacctgatc ccgcagttga ccattgccga aaacattttc
    10981 ctcggtcgtg agtttgttaa tcgctttggc aaaattgact ggaaaaccat gtatgccgaa
    11041 gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagct ggtgggcgat
    11101 ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt tgagtcgaaa
    11161 gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga atccctgttc
    11221 cgcgtcatcc gcgaactaaa atcgcaaggc cgcggtattg tctatatctc ccaccgcatg
    11281 aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgacgggca atttattgct
    11341 gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt gggtcgcaag
    11401 ctggaagatc aatatccgca cctggacaaa gcgccgggag atatccgcct gaaagtcgat
    11461 aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg cgaaattctt
    11521 ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct ctacggcgca
    11581 ctaccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac ccgttcaccg
    11641 caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg tgacggttta
    11701 gtgttgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta cttcagccgc
    11761 gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt cattcgtctg
    11821 tttaatgtga aaacaccgtc gatggaacag gcaattggtc tgctttccgg tggcaatcag
    11881 caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtgttgat ccttgatgaa
    11941 cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat taaccagttc
    12001 aaagccgatg gcttgagcat cattctggtg tcatcggaga tgccagaagt attaggcatg
    12061 agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac tcgtgagcag
    12121 gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag
    12181 taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat
    12241 ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga cgttaagccc
    12301 gaactttttc accatcaata acttattcaa tattctccag caaacctcgg tgaacgccat
    12361 tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atcgacttgt ctgtaggttc
    12421 tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag tcaatgcgct
    12481 ggtggctgtc gctgctgctc tcgcgttagg tgccgcaatt ggtgcggtaa ccggggtgat
    12541 tgtagcgaaa ggtcgcgttc aggcgtttat cgctacgctg gttatgatgc ttttactgcg
    12601 cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta ctgagaacgc
    12661 cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc cagtctggat
    12721 catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc tggggcgtta
    12781 catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca acgtcaataa
    12841 aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg ggatcattga
    12901 agtggcgcgt ctctcctccg cacaacccac ggcggggact ggctatgagc tggatgctat
    12961 tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac
    13021 gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt taggtgtttc
    13081 ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa
    13141 caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa aactggctac
    13201 cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg caaaagacac
    13261 catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga aagatggcgc
    13321 gcagaaagag gcggataagc ttggctataa cctggtggtg ctggactccc agaacaaccc
    13381 ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa ttctgctgat
    13441 taacccgacc gactccgacg cagtaggtaa tgctgtgaag atggctaacc aggcgaacat
    13501 cccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc acattgcttc
    13561 tgataacgta ctgggtggca aaatcgccgg tgattacatc gcgaagaaag cgggtgaagg
    13621 tgccaaagtt atcgagctgc aaggcattgc tggtacatcc gcagcccgtg aacgtggcga
    13681 aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc agccagcaga
    13741 ttttgatcgc actaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt
    13801 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcgcg cactgcaaac
    13861 tgccggtaaa tcggatgtga tggtcgtcgg atttgacggt acaccggatg gcgaaaaagc
    13921 ggtgaatgat ggcaaactag cagcgactat cgctcagcta cccgatcaga ttggcgcgaa
    13981 aggcgtcgaa accgcagata aagtgctgaa aggcgagaaa gttcaggcta agtatccggt
    14041 tgatctgaaa ctggttgtta agcagtagtt ttaatcaggt tgtatgacct gatggtgaca
    14101 taaatacgtc atcgacagat gaacgtgtaa tataaagaaa agcagggcac gcgccaccct
    14161 aacacggtgg cgcattttat ggacatcccg aatatgcaaa acgcaggcag cctcgttgtt
    14221 cttggcagca ttaatgctga ccacattctt aatcttcaat cttttcctac tccaggcgaa
    14281 accgtaaccg gtaaccacta tcaggttgca tttggcggca aaggcgcgaa tcaggctgtg
    14341 gctgctgggc gtagcggtgc gaatatcgcg tttattgcct gtacgggtga tgacagcatt
    14401 ggtgagagcg ttcgccagca gctcgccact gataacattg atattactcc ggtcagcgtg
    14461 atcaaaggcg aatcaacagg tgtggcgctg atttttgtta atggcgaagg tgagaatgtc
    14521 atcggtattc atgccggcgc taatgctgcc ctttccccgg cgctggtgga agcgcaacgt
    14581 gagcgtattg ccaacgcgtc agcattatta atgcagctgg aatcaccact cgaaagtgtg
    14641 atggcagcgg cgaaaatcgc ccatcaaaat aagactatcg ttgcgcttaa cccggctccg
    14701 gctcgcgaac ttcctgacga actgctggcg ctggtggaca ttattacgcc aaacgaaacg
    14761 gaagcagaaa agctcaccgg tattcgtgtt gaaaatgatg aagatgcagc gaaggcggcg
    14821 caggtactgc atgaaaaagg tatccgtact gtactgatta ctttaggaag tcgtggtgta
    14881 tgggctagcg tgaatggtga aggtcagcgc gttcctggat tccgggtgca ggctgtcgat
    14941 accattgctg ccggagatac ctttaatggc gcgttaatca cggcgttgct ggaagaaaaa
    15001 ccattgccag aggcgattcg gtttgcccat gctgccgctg cgattgccgt aacacgtaaa
    15061 ggcgcacaac cttccgtacc gtggcgtgaa gagatcgacg catttttaga caggcagagg
    15121 tgacgcttgg ctacaatgaa agatgttgcc cgcctggcgg gcgtttctac ctcaacagtt
    15181 tctcacgtta tcaataaaga tcgcttcgtc agtgaagcga ttaccgccaa agttgaagcg
    15241 gcgattaaag aactcaatta cgcgccatca gccctggcgc gtagcctcaa actcaatcaa
    15301 acacatacca ttggcatgtt gatcactgcc agtaccaatc ctttctattc agaactggtg
    15361 cgtggcgttg aacgcagctg cttcgaacgc ggttatagtc tcgtcctttg caataccgaa
    15421 ggcgatgaac agcggatgaa tcgcaatctg gaaacgctga tgcaaaaacg cgttgatggc
    15481 ttgctgttac tgtgcaccga aacgcatcaa ccttcgcgtg aaatcatgca acgttatccg
    15541 acagtgccta ctgtgatgat ggactgggct ccgttcgatg gcgacagcga tcttattcag
    15601 gataactcgt tgctgggcgg agacttagca acgcaatatc tgatcgataa aggtcatacc
    15661 cgtatcgcct gtattaccgg tccgctggat aaaactccgg cgcgcctgcg gttggaaggt
    15721 tatcgggcgg cgatgaaacg tgcgggtctc aacattcctg atggctatga agtcactggt
    15781 gattttgaat ttaacggcgg gtttgacgcc atgcgccaac tgttatcaca tccgctgcgt
    15841 cctcaggccg tctttaccgg aaatgacgct atggctgttg gcgtttacca ggcgttatat
    15901 caggcagagt tacaggttcc gcaggatatc gcggtgattg gctatgacga tatcgaactg
    15961 gcaagcttta tgacgccacc attaaccact atccaccaac cgaaagatga actgggggag
    16021 ctggcgattg atgtactcat ccatcggata acccagccga cccttcagca acaacgatta
    16081 caacttactc cgattctgat ggaacgcggt tcggcttaga tttacgctgt cttttgatca
    16141 aattattacc atcggttgtt ttcagaagca tgaacattgc tgctgaagca acagtaataa
    16201 tgcccatcgt gataaacgta tagtggaatt gttcgacagt cgttgtgcct tccatccctt
    16261 cataaacgcg aaggacggcc gcacttacag caacgcctaa actaatcgac agttgctgcg
    16321 tgaccgccag aacactgtta ccgctgctgg cgttgtcatc ggtcagatcg gcaagtgtga
    16381 tggtattcat cgcggtaaac tgcgtcgaca tagccatccc taatataaac aacggcaaga
    16441 tcagcatcca tatcgccatt gccggtgatt gcaaagagaa ctgagcgatc attagcccaa
    16501 taatcaccgt gatcccaact aacgtatggc gatagcccag acgacgtaag acttgggtaa
    16561 ccatcgattt tgcaataatg gaacctaacg ctgtcggtgc catcatacag ccggcaataa
    16621 acgcctgata accaaatcct acctgtaaca tcaatggcat aaggaacggt acacagccgg
    16681 tccccagacg ggttgcaata ttgcctacga taccgatcga gaaagtgcgg gttttaaata
    16741 aatataatga aattaatggg tttggcgtgt gtcgcgcatg gagaatatag agaagcagta
    16801 acccgatgct ggtgacaatt accgtcaagg caatccagct ggcgacaatc ttttccccga
    16861 atagctctat tccgcttgag aagagaacaa ggctgaggcc aaacagcaaa aagccagtga
    16921 tatcgaatct gcgtcgtgcg gtggtgaaat tgggcatatg tttgcgcgcg taaagaaggc
    16981 ccgcaatacc tatggggata ttgattaaaa atatccagtg ccaggttgcc caggtgacca
    17041 gcacgccgcc aagaacgggg cctaaaattg gccccaccag acccggcatg gcgacaaaat
    17101 tcaatactgg aagaagttca ttacgaggat aagcgcgcag taaggccagc cgagcaacag
    17161 gcatcatcat tgcgccgcct atcccctgaa taacccggaa gacaaccagc tgtggtagcg
    17221 aattagaaag tgcgcaggcc agagaaccca atgtgaacag actcacggca agggtaaaaa
    17281 tgcgacgcgt accgaagcga tcggctagcc atccgcttac cggaataagc atcgccaccg
    17341 tcagcgtata actgatgatg gctgattgca tcgcgagagg agaacgatta aggctatgag
    17401 cgattgcggg taaggcggta ttcagaatag tggcatcaag tgcctgcatg aagaaggcca
    17461 tcgccgcgat ccacggcaaa cccgccatac tgcgcttctt tttatcgctc attcaatgtc
    17521 ctgttatcgg gttgtcactt atcaggtgag cgtagcagcg cctgacaagc tttaaatgcc
    17581 gcgtcgccat cgctttggac aatcgcatcg acaatcgcct ggtgcagatc cagctttatc
    17641 actgtgtcgc tggtaattga cgtgaagtaa gtgtgataaa ccgaatggaa tagcgaggcg
    17701 aatgaggtca aaaacggatt ggcgctcatt tcatagatat gctcatgcca ggccatatct
    17761 acttcgatcc agcgttcacg gcgaaagttc tcttttaatg ccgccatttc ggccattaac
    17821 gtattgagat gcgccttctg ttccgcggtg ccaaccgttg cagccagtag gcaagcttgc
    17881 ggctccagac agatacgcat aaccagaaag tgatcgatga cctgatgaaa gttctcttct
    17941 gtcatccacc aggtaagcaa ttcctgatca agaaaattcc agtttgattg tggcatgacc
    18001 cgggtaccaa ttcgcggtcg cggtaaaacc atcccttttg ccgttaacgt tttgaccgct
    18061 tcgcgtaccg ctgtacgact cactccaaat tgctcgccca gctcaatctc accaggcaaa
    18121 atggtgccgg gttcatattc accttttaag atgcgttgcg ccagcttctc agccagaaca
    18181 tacgaaaggt ttttctgtgc agctaactgt tgtgcgctta aaggcattac taatcttcct
    18241 ttttcttttt attcctcctt agtatgccac caggaagtgt gattacggtt gcaaaaacgg
    18301 caaattgctt gttttatggc acattaacgg ggcttttgct gaaaaaatgc gcggtcagaa
    18361 aattatttta aatttcctct tgtcaggccg gaataactcc ctataatgcg ccaccactga
    18421 cacggaacaa cggcaaacac gccgccgggt cagcggggtt ctcctgagaa ctccggcaga
    18481 gaaagcaaaa ataaatgctt gactctgtag cgggaaagcg tattatgcac acccccgcgc
    18541 cgctgagaaa aagcaaagcg gcactgctct ttaacaattt atcagacaat ctgtgtgggc
    18601 actcgaagat acggattctt aacgt
//
LOCUS       DAOFQD010000093        18413 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.093, whole genome shotgun sequence.
ACCESSION   DAOFQD010000093 DAOFQD010000000
VERSION     DAOFQD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.093"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            330..2753
                     /gene="pgaA"
                     /locus_tag="QBF46_003316"
                     /pseudo
     CDS             330..2753
                     /gene="pgaA"
                     /locus_tag="QBF46_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2762..4780
                     /gene="pgaB"
                     /locus_tag="QBF46_003317"
     CDS             2762..4780
                     /gene="pgaB"
                     /locus_tag="QBF46_003317"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDQ6675465.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIRDAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEYSTAWYPKND"
     gene            4773..6098
                     /gene="pgaC"
                     /locus_tag="QBF46_003318"
     CDS             4773..6098
                     /gene="pgaC"
                     /locus_tag="QBF46_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDQ6675466.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6100..6513
                     /gene="pgaD"
                     /locus_tag="QBF46_003319"
     CDS             6100..6513
                     /gene="pgaD"
                     /locus_tag="QBF46_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDQ6675467.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6563..7351)
                     /gene="phoH"
                     /locus_tag="QBF46_003320"
     CDS             complement(6563..7351)
                     /gene="phoH"
                     /locus_tag="QBF46_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDQ6675468.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7959..9230)
                     /gene="efeB"
                     /locus_tag="QBF46_003321"
     CDS             complement(7959..9230)
                     /gene="efeB"
                     /locus_tag="QBF46_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDQ6675469.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGRALLQV"
     gene            complement(9236..10363)
                     /gene="efeO"
                     /locus_tag="QBF46_003322"
     CDS             complement(9236..10363)
                     /gene="efeO"
                     /locus_tag="QBF46_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDQ6675470.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10421..11251)
                     /locus_tag="QBF46_003323"
     CDS             complement(10421..11251)
                     /locus_tag="QBF46_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDQ6675471.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHVQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11793..13301)
                     /gene="putP"
                     /locus_tag="QBF46_003324"
     CDS             complement(11793..13301)
                     /gene="putP"
                     /locus_tag="QBF46_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDQ6675472.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFSSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13460..13669)
                     /locus_tag="QBF46_003325"
     CDS             complement(13460..13669)
                     /locus_tag="QBF46_003325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675473.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13724..17686
                     /gene="putA"
                     /locus_tag="QBF46_003326"
     CDS             13724..17686
                     /gene="putA"
                     /locus_tag="QBF46_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDQ6675474.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKVNIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVTPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDSQLKAALTQPLNAL
                     REWAANRPELQALCTQYGEQAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17726..18364)
                     /gene="rutR"
                     /locus_tag="QBF46_003327"
     CDS             complement(17726..18364)
                     /gene="rutR"
                     /locus_tag="QBF46_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDQ6675475.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         4669 a         4751 c         4526 g         4467 t
ORIGIN      
        1 cgtagcgcag cctttcatca ggactttcga aaaatccgaa atcatgcatc ggaatttact
       61 gatttaatta ttttaatcct aatttatttt gaaaaaggca ttgggattta tgccgtattc
      121 ctgaagatcc tcatcattgg aatggatttt cgggcgagaa aaggatttta tatggacact
      181 ctgctcatca tttcttcttc tcatcatcaa caattcacgt ctctcttccg cgtttaataa
      241 cggattatga ggtgcaaaaa tatctttctt ttcagttacc tgtaattaga tacagagaga
      301 gattttggca atacatggag taatacagga tgtattcaag tagcagaaaa aggtgcccga
      361 aaaccaaatg ggctttgaaa cttcttactg ccgcattttt agcagcgagt cccgcggcga
      421 agagtgctgt taataacgcc tatgatgcat tgattattga agctcgcaag ggtaatactc
      481 agccagcttt gtcatggttt gcactaaaat cagcactcag caataaccaa attgctgact
      541 ggttacagat tgccttatgg gccgggtaag ataaacaggt tattaccgtt tacaaccgct
      601 accgtcatca gcaattacca gcgcgtggtt atgcagctgt cgccgtcgct tatcgtaacc
      661 tgcaacaatg gcaaaactcg cttacactgt ggcaaaaggc gctctctctg gagccgcaaa
      721 ataaggatta tcaacgggga caaattttaa ccctggcaga tgctggtcac tatgatactg
      781 cgctggttaa acttaagcag cttaactctg gagcaccgga caaagccaat ttactcgcag
      841 aagcctatat ctataaactg gcggggcgtc atcaggatga attacgggcg atgacagagt
      901 cattacctga aaatgcatct acgcaacaat atcccacaga atacgtgcag gcattacgta
      961 ataatcaact tgctgccgcg attgacgatg ccaatttaac gccagatatt cgcgctgata
     1021 ttcatgccga actggtcaga ctgtcgttta tgcctacgcg cagtgaaagt gaacgttatg
     1081 ccattgccga tcgcgccctc gcccaatacg ctgcattaga aattctgtgg cacgataacc
     1141 cagaccgcac tgcccagtac cagcgtattc aggttgatca tcttggcgcg ttattaactc
     1201 gcgatcgtta taaagacgtt atttctcact atcagcgatt aaaaaagacg gcgcaaatta
     1261 ttccgccctg ggggcaatat tgggttgcat cggcttatct caaagatcat cagccgaaaa
     1321 aagcacagtc aataatgacc gagctctttt atcacaagga gaccattgcc ccggatttat
     1381 ccgatgaaga acttgcggat ctcttttaca gccacctgga gagtgaaaat tatccgggcg
     1441 cgctaactgt cacccaacat accattaata cttcgccgcc tttccttcgg ttaatgggca
     1501 cgcctacgag catcccgaat gatacctggt tacaggggca ttcgtttctc tcaaccgtag
     1561 caaaatatag taatgatctt cctcaggctg aaatgacagc cagagagctt gcttataacg
     1621 caccaggaaa tcagggactg cgcattgatt acgcgagtgt gttacaagcc cgcggttggc
     1681 ctcgtgcagc agaaaatgaa ttaaaaaaag cagaagtgat cgagccacgt aatattaatc
     1741 tggaggttga acaagcctgg acagcattaa cgttacaaga atggcagcag gcagctgtct
     1801 taacgcacga tgttgtcgaa cgtgaaccgc aagatcccgg cgttgtacga ttaaaacgtg
     1861 cggttgatgt acataatctt gcagagcttc gtatcgctgg ctcaacagga attgatgccg
     1921 aaggcccgga tagtggtaaa catgatgtcg acttaaccac catcgtttat tcaccaccgc
     1981 tgaaggataa ctggcgcggt tttgctggat tcggttatgc cgatggacaa tttagcgaag
     2041 gaaaagggat tgttcgcgac tggcttgcgg gtgttgagtg gcggtcacgt aatatctggc
     2101 tcgaggcaga gtacgctgaa cgcgttttca atcatgagca taaacccggc gcgcgcctgt
     2161 ctggctggta tgattttaat gataactggc gtattggttc gcaactggaa cgcctctctc
     2221 cccgcgttcc attacgggca atgaaaaatg gtgttacagg caacagtgct caggcttatg
     2281 ttcgctggta tcaaaatgag cggcgtaagt acggtgtctc ctgggctttc actgattttt
     2341 ccgacagtaa ccagcgtcat gaagtctcaa ttgagggtca ggaacgcatc tggtcttcac
     2401 catatttgat tgtcgatttc ctacccagtc tgtattacga acaaaataca gaacacgata
     2461 ccccatacta caaccctata aaaacgttcg atattgttcc ggcatttgag gcaagtcatt
     2521 tgttatggcg aagctatgaa aatagctggg agcaaatctt cagcgcaggt gttgatgcct
     2581 cctggcaaaa acattatggc acggatgtcg tcacccaact cggctacggg caacgcatta
     2641 gctggaatga cgtgattgat gctggcgcaa cgctacgctg ggaaaaacga ccttatgacg
     2701 gtgacagaga acacaactta tacgttgaat tcgatatgac attcagattt taaggataaa
     2761 tatgttacgt aatggaaata aatatctcct gatgctggtg agtataatta tgctcaccgc
     2821 gtgcatcagc cagtcaagaa catcatttat accgccacag gatcgcgaat ctttactcgc
     2881 cgagcaaccg tggccgcata atggttttgt ggcgatttca tggcataacg ttgaagacga
     2941 agctgccgac cagcgtttta tgtcagtgcg gacatcagca ctgcgtgaac aatttgcctg
     3001 gctgcgcgag aacggttatc aaccggtcag tattgctcaa attcgtgatg cacatcgagg
     3061 aggaaaaccg ctaccggaaa aagcagtagt gctgactttt gatgacggct accagagttt
     3121 ttatacccgc gtcttcccaa ttcttcaggc cttccagtgg cctgctgtat gggcccccgt
     3181 cggcagttgg gtcgatacgc cagcggatga acaagtaaaa tttggcgatg agatggtcga
     3241 tcgagaatat tttgccacgt ggcaacaagt gcgagaagtt gcgcgttccc ggctcgttga
     3301 ggtcgcttct catacatgga attctcacta cggtattcag gctaattcca ccggcagctt
     3361 aatgcctgta tatgtaaatc gtgcatattt tactgaccac gcacggtatg aaaccgcagc
     3421 agaataccgg gaaagaattc gtctggatgc tgtaaaaatg acggaatacc tgcgtacaaa
     3481 ggctgaggta aatccacacg tttttgtttg gccttatggc gaagcgaatg gcatagcgat
     3541 agaggaatta aaaaaactcg gttatgacat gtttttcacc cttgaatcag gtttggcaaa
     3601 tgcgtcacaa ttggattcca ttccgcgagt attaatcgcc aataatccct cattaaaaga
     3661 gtttgcccag caaattatta ccgtacagga aaaatcacca caacggataa tgcatatcga
     3721 tcttgattac gtttatgacg aaaacctcca gcaaatggat cgcaatattg atgtgctaat
     3781 tcagcgggtg aaagatatgc aaatatcaac cgtgtatttg caggcatttg ctgatcccga
     3841 tggtgatggg ctggtcaaag aggtctggtt tccaaatcgt ttgctaccaa tgaaagcaga
     3901 tatttttagt cgggttgcct ggcaattacg tacccgctca ggtgtaaaca tctatgcgtg
     3961 gatgccggta ttaagctggg atttagatcc cacattaacg cgagtaaaat acttaccaac
     4021 aggggagaaa aaagcacaaa ttcatcctga acaatatcac cgtctctctc ctttcgatga
     4081 cagagtcaga gcacaagttg gcatgttata tgaagatctt gccggacatg ctgcttttga
     4141 tggcatattg ttccacgatg atgctttgct ttcagattat gaagatgcca gtgcaccggc
     4201 tatcacggct tatcagcaag caggctttag cgggagtctg agcgaaattc gacaaaaccc
     4261 ggagcaattt aaacagtggg cccgctttaa aagtcgtgcg ttaactgact tcactttaga
     4321 acttagtgcg cgcgtaaaag ccattcgcgg tccacatatt aaaactgcac gaaatatttt
     4381 tgcacttccg gtaatacaac ctgaaagtga agcctggttt gcacagaatt atgctgattt
     4441 cctaaaaagc tatgactgga ccgctattat ggctatgcct tatatggaag gtgtcgcaga
     4501 aaaatcggct gaccaatggt taatacaatt gaccaatcaa attaaaaaca tccctcaggc
     4561 taaagacaaa tctattttag aattacaggc acaaaactgg cagaaaaatg gtcagcatca
     4621 ggctatttct tcgcaacaac tcgctcactg gatgagccta ttacaactga atggagtgaa
     4681 aaactatggt tattatcccg acaattttct gcataaccaa cctgaaatag accttattcg
     4741 tcctgagtat tcaacagcct ggtatccgaa aaatgattaa tcgcatcgta tcgtttttta
     4801 tattatgtct ggtgttatgc atacccctat gcgtagcgta ctttcactct ggtgaactga
     4861 tgatgaggtt cgttttcttc tggccgtttt ttatgtccat tatgtggatt gttggcggcg
     4921 tctatttctg ggtctatcgt gaacgccact ggccgtgggg agaaaacgca ccagctcccc
     4981 agttgaaaga taatccgtct atctccatta tcattccctg ttttaatgag gagaaaaacg
     5041 ttgaggaaac catacacgcc gctttagcac agcgttatga gaacattgaa gttattgccg
     5101 taaatgacgg ttcaacagat aaaacccgtg ccatcctgga tcgcatggct gcacaaattc
     5161 cccatttgcg ggtcattcat ctggcgcaaa accaggggaa agccattgcg cttaaaaccg
     5221 gagctgccgc ggcgaaaagt gaatatctgg tgtgcattga tggcgatgcg ttattagacc
     5281 gcgatgcggc ggcatatatt gtggaaccga tgttgtacaa cccgcgtgtg ggtgccgtaa
     5341 ccggtaatcc tcgtattcga acacgttcta ccctggtggg taaaattcag gttggcgagt
     5401 attcctcaat tattggtttg atcaagcgaa cccagcgtat ctatggaaac gtatttaccg
     5461 tttccggtgt tattgccgca tttcgtcgca gcgccctggc agaagtgggt tactggagtg
     5521 acgatatgat caccgaagat attgatatta gctggaagct gcagttgaat cagtggacga
     5581 ttttttacga gccacgggcg ctgtgctgga tattaatgcc tgaaacgtta aaagggctgt
     5641 ggaaacagcg cctgcgctgg gctcagggcg gtgcagaagt attcctcaaa aatatgacaa
     5701 ggttgtggcg caaagaaaac tttcgaatgt ggccgctgtt ttttgaatac tgcctgacga
     5761 caatatgggc cttcacctgc ctggtcggtt tcattattta cgcagtccaa cttgccggtg
     5821 taccgttaaa tattgaattg acacatatcg ctgcgacaca tactgccgga atattattgt
     5881 gtacgttatg tttactgcaa tttattgtca gcctgatgat cgagaatcgc tatgagcata
     5941 atctgacttc atcgcttttc tggattattt ggttcccggt tattttctgg atgctgagcc
     6001 tggcaacgac attggtatca tttacacgag tcatgttgat gcctaaaaag caacgcgccc
     6061 gttgggtaag tcccgatcgc gggattctga gaggttaata tgaacaattt aattattacg
     6121 acccgacaat caccagtacg tttactggtt gattatgttg ccacaaccat cttgtggaca
     6181 ttatttgcgt tgttcatatt cttattcgcc atggatctgc tgacgggtta ttactggcaa
     6241 agcgaggcca gaagccgact tcagttctat tttttgctgg cagtggcgaa tgccgtcgtg
     6301 ttaattgtct gggcgctgta caataagctg cgttttcaaa aacagcagca tcatgcagcc
     6361 taccaatata cgccgcaaga atatgcagag agcttagcaa tacctgatga gctctatcag
     6421 caactacaaa aaagccacag gatgagcgta cacttcacta gccaggggca aataaaaatg
     6481 gttgtttcag aaaaagcgct agtccgggca taaacaccca aaacaaagcc cggttcgccc
     6541 gggctctgca ccgataacac acttaactgt aggcatgcag cgtacgttgg caaagtgccg
     6601 aacgtacgca atcctcttta ccgaatcgga tgatcccgac catttcatct tcttcgaaac
     6661 gttccagcgc gtcacttaat ccggagcgca caccgcgagg taaatcgcat tgtgtgatat
     6721 caccgttgac gataaccgtc acgttctccc cgaggcgggt taaaaacatt ttcatttgcg
     6781 cggcagtcac attctgcgcc tcgtcaagaa tgacgactgc attttcaaag gtacgtccac
     6841 gcatataggc gaacggcgcg atttccacct tccctatttc cggtcgcagg cagtactgca
     6901 taaaggaagc ccctaagcgc cggaccagca cgtcgtagac cgggcgaaaa tagggagcaa
     6961 acttttctgc gatatctcca ggtaagaagc caagatcttc atcggcttgc agaactggac
     7021 gggtgacgat aatcctgtcg acatccttat gtatcagggc ctctgccgct tttgctgcgc
     7081 tgatccaggt ttttccgcac ccggcttcgc ccgtagcgaa tatcagctgc ttactctcaa
     7141 tagccttcag atagtgcaat tgcgcttcat ttcgcgcgag gatgggcgag gtatcgcgac
     7201 tgtcgcgggc cataccaatg gcttctacgc cgctcatctg cacaagcgag gtgaccgatt
     7261 cttcttcacg ctgcttatgg ctgcgcgaat cccgtctcag cacacgtttt gcctcgcgac
     7321 gagctttgat cactgctttt tgtcttccca tggagagcac cttgagttgt ttgtattcat
     7381 cacacgcgcc gttggcagcg cgattatgcg cacgaacatc agagggttgg cttccttgta
     7441 agccatagtt tgcttttgga taaaatgccg aaaaacggct acgcacaccg tttgcggcgt
     7501 cggtaacacg agtaaagaga ggaagtgaga aaaatacaga gtgacgaagt tgctaccgga
     7561 ggagaaatct ccgcgagtgg tggagcgttg cttaacaaaa cattgtccag aacaggacgt
     7621 taccatccgc gatctccata gtgactgact atcactgccg ggaacttccg ctgctactta
     7681 ataattacaa cagaactcgc attcattgca acaatatatt cacttatatt taacgataaa
     7741 cacccatttc ggtgacatta gtttctactg gaaagatgac agagtgatga cagtgatgga
     7801 aaaagctgtg tgctttcagc aggattcgca gggctggtaa aatgtgcctc gtgtttgccg
     7861 gattcggcgc gaacgcctta cccgacctac aagtgattaa taatttgtgc acaaaactca
     7921 tgcccgataa acctgcgcat cgggcatatt tgcgtcgttc aaacctgcaa tagcgccctt
     7981 cccagataat cgttcgcgtc cttcactccc ggcagcgcaa aaaaataacc gccgccgata
     8041 ggtttaacgt attcctccag cgcttcgcca ttgagccttt tttgtactgt caggaagcct
     8101 ttttccagat cgtgttggta gcagacaaac agcaacccca tatccagttg cccggagttg
     8161 gtgacgccca gtgaataact gtagccacga cgcagcatca ggctggactc actctccgcc
     8221 gtgcggggat tcgccagccg gatatggctg tccagcgcga tcaccttccc ttccgggtcg
     8281 ctggcgtaat caggcacatc atgctcatgc tgcattccca gcggcgcacc ggtttgctta
     8341 tcacggccaa aaatcgtctg ctgttctttc agcggcgttc tgtcccaaaa ttccactcga
     8401 aactgaatca agcgtactgc ctgatagctg ccaccgattg tccacgcagg ctcctgctga
     8461 tctgccgtta cccacaccac tttttgcatc aacttatcat tctggctatc gggattggca
     8521 gtgccgtctt tgaaacccag caaattaatc ggcgtctctt tgcctttact acgcgccgcg
     8581 tgatcggaaa taaacccttc ccgcttccag cgcacactga gcaaatccgg cgtgtgtttg
     8641 atgatatcgc gcagcgcatg gataaccgtg tcctgggtgt tggcgcaaat ctgtagcaac
     8701 acatcaccat gacataacgc cgcatccagc gagtcgttgg ggaaacgcgt catcttctgc
     8761 agcttttttg gcatctgtgg cgcaaggcca aagcgctcat caaacaatga gtgacccacc
     8821 gataacgtga tggtgagatt atcgggcgca atgtagccgc caagaatgcc ggaatcgagt
     8881 ggtggcaggc gcggatttgg cgtttctggt gctgctccgc cctgagtcag aaaagcaaaa
     8941 cgctgagtca acaagcgaaa caaccgctca agatcggctt tatcgctggc aagcacatca
     9001 aacgccacca gcatcattgc ggcctgttgt ggcgtcagga tccctgcctg atgctcacca
     9061 taaaacggct gtttctcatt gcgagcatcc ggtgaaagcg tacccggcgc actttgcgtt
     9121 ttttgtgcat gagcgaccgg acaacttccc gccagcgcca gtgcacctat cactttcagt
     9181 aaacgtcggc gtgacggttc attcacgccg ttttcatctt tatactgcat aacgcttaat
     9241 ccagtcccag cacaccgcga agttgcgcca gatcttccgc cagcgcagta atcggtcctt
     9301 tcagtgcatt ccggtcggca tcggtcaatt tgtcgtaggt ttcaaaaccg tctttagtac
     9361 ggtatttcgc cagaatggta tcgacctttt taaagttggc atcgactttt gccagcagtt
     9421 ccgggttggc tttttgcagt tgtggacgca gcaaatcgac aattttctgc gagccttcaa
     9481 cgttagcctg gaaatcccac agatcggtgt ggctgtagcg atcttcttca ccgctaattt
     9541 tgctggctgc cacttcctca atcagtccgg ctgcgccgcc gaccactttt gaaggtggga
     9601 aagccagttc actgatgcgt ttttgcaaat cgaccacatc ggtataaagc tgctcagcgt
     9661 actgatccat ccctttggtg gtgttgtcgc caaacaatgc tttttccaga cggtggaaac
     9721 cagtgaattt tgggtcggcg gctttttgct cgtaatcatc ttcacgggcg tcaatgctgc
     9781 catccagatc ggagaacagt tcagcaatcg gttcaatacg ctcatagtgc tggcgcgtcg
     9841 gtgcatacag tgctttcgct ttttcgatat cgcctgcttt aatcgcgtcg gtaaaggctt
     9901 tggtgtcggt caccagctgc gtggtttccg ccatgacata cgctttatat gcagtaattg
     9961 caccaccaag acttaacagc gcatcacttt gcgccgcatc cgccgttgcc tcacctttga
    10021 cgatcaactt ccctttcggg ttagtcagca gaccgcaggt catatcgtat tcgccaggct
    10081 gtaaattcgc cgtcattttc tggctaaagc caggggcgat attttcccgc tcttccacca
    10141 ccatcacgcc tttgaggatc tcccactcca gcgccttctg gctgtggttc tgaataatga
    10201 actgtgtttt cccggcgtta accgtaatgg tcatcggttc gcactgctta tccgtcacgg
    10261 tcactttgac ctgcggcaca tcagcggcgt tagccataaa agcagaagaa aacagcgcag
    10321 ccacgctcaa ctgcaatgcg ttacggcgga agttaatggt catgacgaga ctatcccttt
    10381 aaagtatgtt gtaactaagc aagagtttgc gtcgtatttg ttacgcggag cgagacgctg
    10441 tcgcccctgc gcgtggtggc agagcaaatg ccaccagcgc cgggatgaga taaataaacc
    10501 agacggcgac ttcgctgacg ctcggcgctt cctgatagcc aaaaatccct tccatcagcg
    10561 tgccaaacag cgagtgagtt gagagcaccg cactcatatc gaaggcgatt tcctgaacgt
    10621 ggttccacaa tccggcttca tgaaatgcgc gaatggcacc agctgccagc cctgcggcga
    10681 cgaagagaat aaacaggctg gtccatttaa aaaatgcacc aagattgagg cgaataccgc
    10741 cccagtagag caggaagcct agcaccacgg cagtagcaag accgagcatt gcacccagcg
    10801 gcggccagat cccgacatct tgttgaaatg ccgccagcag gaaaaagacc gactccagcc
    10861 cttcccttgc aacggcaaaa aagaccatca tcaccagcgc ccagccatga tgatttccac
    10921 gctgcaatgc gctatcgact gcctgttcca gttgcacttt gacgttgcgc gacactttgc
    10981 gcatccagaa aaccatccag gtaaggatca ccacggcgat caccgccacg ataccttcaa
    11041 acagttcctg ttctttttgc ggaaattcgc cggtggtttc gttaatgaag atacccaagc
    11101 ccaggcacaa cgcagcggca agcaacacgc caatccacat cacaccaatc catcggcctc
    11161 gctgggtacg cttaagatag ctggcaatca aactgacaat cagcgcggct tcaagtcctt
    11221 cgcgcaacat aatgagaaac ggaacaaaca tgccacccac ccttaaacgt ttttctgagt
    11281 caatctatgc aaagaaaagt aaatcgcagt gatagcgatt atcattacgg taaagaaaaa
    11341 tacaagcgga atgtttataa aatgatgttg aaatgtaaac aaagagaggt aatgccaggg
    11401 gatataaacg attattccag gtacaaagat gggattgttt acaacgagcc atgtgttctt
    11461 gccggatgcc gcgtaaacgc cgtatccagc cgacaaaact gtccagactc aatgcatcag
    11521 agcttccatg taaaactgat aagcgggtgt atcaagcaat ttcgtccatc cgcgattatc
    11581 aaatctaact ttttgcccgg aacataataa cagctcgggt gttggtggcg tcaaaatcgc
    11641 tgaagcgttc cccgaaggcc gggacagcag ggggcggcgg cgattggccg ccccctgcgc
    11701 gctccctgca ccattcaatt tatatggcga ggcacattaa gggagcgcaa cccgctgctg
    11761 aagccaaaca aaaccgccgc aggctaagtc ccttagcttt cctgcaaccg tgacggcgga
    11821 gccgaatgat agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct
    11881 ttacccagca aactaaacac tacaatccca atactgctga agataaagcc cggaataatt
    11941 tcgtacagtc ccagccagcc gaactgtttc cagacgataa ccgtcagcgc cccgatgatc
    12001 atccccgcca gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc
    12061 ggaccaaacg ccgcgccaaa gcctgcccac gcatagctca ctagccccag cacacggttt
    12121 tccgggtttg ccgccagcgc aatggccacc agcgccacca ccagcaccat cacacgacct
    12181 acccacacca gctctttctg gctggcatgt ttacgcagaa acgctttgta taaatcttcg
    12241 gtaatcgcac tggagcacac cagcagctgg caacttaagg ttgacattac cgccgccaga
    12301 attgccgaca gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata
    12361 aacacgcgct cagcattctg gtttaccgca cctgctaccg ccgggtgctc attaaagtaa
    12421 gcaataccaa agaagccgac agctaccgcc cctgccaggc agaggatcat ccaggtcata
    12481 ctgatacgac gcgcatggac aatgctgtgg tgagagtctg ccgccataaa acgcgccagg
    12541 atgtgcggtt gcccgaagta acccagcccc caacccatca gtgagataat ggcgacaaag
    12601 ttcagccctt tgagcatatc aacgttttcg atgctctttt gtttgatcac ttccagcgag
    12661 tcaccaaagc caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc
    12721 atcaggctgg cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg
    12781 taaaggatcg tcgccgcagc tcccgcccac agagccgttt cgtagctcat gccaaaggta
    12841 ctttcaaaca gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc
    12901 aaaatcacca gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa gcgcccggtg
    12961 aaataatccg gcaatgttaa ggcgttattg ttgtattcgg tatgcacacg caaccgcccc
    13021 gccaccagct tccagttgat ccacgcgcct aatgtcaggc caatggcgat ccaactttcg
    13081 gaaatcccgg aaagaaaaac cgcgcccggc aaccccatta acagccagcc gctcatatcc
    13141 gacgcacccg ccgataatgc cgtcacgaat ggcccaaggc tacgaccgcc cagaatatag
    13201 tcgtcaaagt ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata
    13261 tagacacaaa atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt
    13321 attatcggca atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt
    13381 atgttttacc agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac
    13441 acaccattta cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc
    13501 tcacattttt tgcggttgca cctttcaaaa atgttaactg ccgcagagaa aaagtctgag
    13561 ttattttttt aatccctgtc atatcgattt cttttattaa catttcattc atttttaagc
    13621 ttgctacgca tgtcacattt aacatggttg cacaaagttg caacatcatg gatatttcac
    13681 gataacgtta agttgcacct ttcagaacaa caggagtaat ggcatgggaa ccaccaccat
    13741 gggggttaag ctggacgacg cgacgcgtga gcgtattaag tctgccgcga cacgtatcga
    13801 tcgcacgcca cactggttaa ttaagcaggc gattttttct tatctcgaac aactggaaaa
    13861 cagcgatact ctgccggagc tacctgcgct gctttctggc gctgccaatg agagcgatga
    13921 agcaccgact ccggcagagg aaccacacca accattcctc gactttgccg agcaaatttt
    13981 gccccagtcg gtttcccgcg ccgcgatcac agcggcctat cgccgcccgg aaaccgaagc
    14041 ggtttctatg ctgctggaac aagcccgcct gccgcagcca gttgctgaac aggcgcacaa
    14101 actggcgtat caactggccg ataaactgcg taatcaaaaa aacgccagtg gtcgcgcagg
    14161 tatggttcag gggttattgc aggagttttc gttgtcatcg caggaaggcg tggcgctgat
    14221 gtgtctggcg gaagcgttgt tgcgtattcc cgacaaagcc acccgtgatg cgttaattcg
    14281 cgacaaaatc agcaacggta actggcaatc acacattggt cgtagcccgt ctttgtttgt
    14341 taatgccgcc acctgggggc tgctgtttac tggcaaactg gtttccaccc ataacgaagc
    14401 cagcctctcc cgctcgctga accgcattat cggtaaaagc ggtgaaccgc tgatccgcaa
    14461 aggtgtggat atggcgatgc gcctgatggg cgagcagttc gtcactggcg aaaccatcgc
    14521 ggaagcgtta gccaatgccc gcaagctgga agagaaaggt ttccgttact cttacgatat
    14581 gcttggcgaa gctgcactga ctgccgcaga tgcacaagcg tatatggttt cctatcagca
    14641 ggcgattcac gccattggta aagcgtccaa tggtcgtggc atctatgaag gtccgggcat
    14701 ttcaatcaag ctgtcggcgc tgcatccgcg ttacagccgc gcccagtatg accgggtaat
    14761 ggaagagctt tacccacgtc tgaaatcact caccctgctg gcgcgtcagt acgatattgg
    14821 tatcaacatt gacgccgaag aggccgatcg tctggagatc tccctcgatc tgctggaaaa
    14881 actctgtttc gagcctgagc tggcaggctg gaacggtatc ggttttgtta ttcaggctta
    14941 tcaaaaacgc tgcccactgg tgattgatta cctgattgat ctcgccaccc gcagccgtcg
    15001 ccgtctgatg attcgcctgg tgaaaggcgc gtactgggat agtgaaatca agcgtgcgca
    15061 gatggacggc cttgaaggct atccggttta tacccgcaag gtgtataccg acgtttcata
    15121 tctcgcctgt gcgaaaaagc tgctggcggt gccgaatcta atctacccgc agttcgcaac
    15181 gcacaacgcc catacgctgg cagcgattta tcaactggct ggtcaaaact actatccggg
    15241 tcagtacgag ttccagtgcc tgcatggtat gggcgagcca ctgtatgagc aggtcaccgg
    15301 gaaagttgcc gacggcaaac ttaaccgtcc gtgtcgtatt tatgctccgg tcggtaccca
    15361 tgaaacgctg ttggcgtatc tggtgcgtcg cctgctggaa aacggtgcta acacctcgtt
    15421 tgttaaccgt attgccgaca cctctttgcc actggatgaa ctggtcgccg atccggtcac
    15481 tgctgtagaa aaactggcgc aacaggaagg gcaaactgga ttaccgcatc cgaaaattcc
    15541 cctgccgcgc gatctttacg gtcacgggcg cgacaactcg gcagggctgg atctcgctaa
    15601 cgaacaccgg ctggcctcgc tctcttctgc cctgctcaat agtgcactgc aaaaatggca
    15661 ggccttgcca atgctggaac aaccggtagc ggcaggtgag atgtcgcccg ttattaaccc
    15721 tgcggaaccg aaagatattg tgggctttgt gcgtgaagcc acgccgcgtg aggtagaaca
    15781 ggcgctggaa agtgcggtta ataacgcgcc aatctggttt gccactcctc cggttgaacg
    15841 cgcggcgatt ttgcatcgcg ctgccgtgct aatggaaagc cagatgcagc aactgattgg
    15901 tattctggtg cgtgaagccg gaaaaacctt cagtaacgcc attgccgaag tgcgcgaagc
    15961 ggtcgatttt ctccactact atgccggaca ggtgcgggat gatttcgcta acgaaaccca
    16021 ccgtccatta gggcctgtgg tgtgcatcag tccgtggaac ttcccgctgg ctattttcac
    16081 cgggcagatt gctgccgcac tggcggcagg taacagcgtg ctggcaaaac cggcagaaca
    16141 aacgccgctg attgccgcgc aagggatcgc cattttgctg gaagcgggtg taccgccagg
    16201 cgtggtgcag ttgctgccag gtcaggggga aaccgtgggc gcgcaactga cgggtgatga
    16261 tcgcgtacgc ggggtgatgt ttaccggttc aaccgaagtc gctacgttac tgcagcgcaa
    16321 tatcgccagc cgcctggacg ctcagggtcg ccctattccg ctcatcgctg aaaccggcgg
    16381 catgaacgcg atgattgtcg attcttcagc actaaccgaa caagtagtaa tagatgtgct
    16441 ggcctcggcg ttcgacagtg cgggtcagcg ttgttcggcg ctgcgcgtgc tgtgcctgca
    16501 agatgagatt gccgaccaca cgttgaaaat gctgcgcggc gcaatggccg aatgccggat
    16561 gggtaatccg ggtcgcctga ccaccgatat cggtccggtg attgatagcg aagcgaaagt
    16621 caatattgag cgccatattc agaccatgcg tagcaaaggc cgtccggtgt tccaggcggt
    16681 gcgggaaaac agtgaagatg cccgtgaatg gcaaagcgga acctttgtca ccccgacgct
    16741 gatcgaactg gatgactttg ccgaattgca aaaagaggtc tttggtccgg tgctgcatgt
    16801 ggtgcgttac aaccgtaacc agctaccaga gctgatcgag cagattaacg cttccggtta
    16861 tggtctgacg cttggcgtcc atacgcgtat tgatgaaacc atcgcccagg tcactggctc
    16921 ggcccatgtt ggtaacctgt acgttaaccg taatatggtg ggcgcagtgg tgggtgtgca
    16981 gccattcggc ggcgaagggt tgtccggcac cgggccaaaa gcaggcggtc cgctctatct
    17041 ctaccgtctg ctggcgaatc gcccggaaag tgcgctggca gtgacgctcg cgcgtcagga
    17101 tgcagagtat ccggtcgatt cgcagttgaa agccgcattg actcagccgc taaatgcact
    17161 gcgggaatgg gcagcaaatc gtccagaatt gcaggcgtta tgtacgcaat atggcgagca
    17221 ggcgcaggca ggaacacaac gattgctgcc agggccgacg ggtgaacgca acacctggac
    17281 gctgctgccg cgtgagcgcg tattgtgtat cgcggatgat gagcaggatg cgctgactca
    17341 gctcgccgcc gtgctggcgg tgggcagcca ggtactgtgg ccggatgacg cgctgcatcg
    17401 tcagctagta aaggcattgc catcggcagt cagcgaacgt attcaactgg cgaaagcgga
    17461 aaatataacc gctcaaccct ttgatgcggt gatcttccac ggtgattcgg atcagcttcg
    17521 cgcattgtgt gaagccgttg ctgcgcggga tggcgcaatt gtttcggtgc agggttttgc
    17581 ccgtggcgaa agcaatatcc ttctggaacg gctgtatatc gaacgttcgc tgagtgtgaa
    17641 taccgctgcc gctggcggta acgccagttt aatgacgatc ggttaaacag gtagccggag
    17701 gatgttacac cctcctccgg catctttaac gtggtcgaat cccctcaata ataatccgct
    17761 gcacgttttc aaccgtttga ttgaaaaata cctcatcgcg caacgttgcg cctgtcaccg
    17821 cctccacctg aggggcgaaa tcggcgtaat gttgagtgga agcccaaatc ataaaaatca
    17881 aatgctgcgg atcaatcggc gcgagtttgc cgcttttgac ccaaccggca atcagcgccg
    17941 atttctcatc aattaatgcc ttcaaatcgc ccgtcagttc atccattaac agcggcgcgc
    18001 ctgccagcat ctccatacag aacaggcgcg aagcctgcgg ataatcgcgt gagacttcca
    18061 gcttcagacg gatgtactct ttgatcgccg ccagcggggc gaaatcttca cgaaacgctt
    18121 ttaacggtgc cagccagata tcgagaatct gccgcagcac ggcaatatac agcgcctctt
    18181 ttgacggaaa gtaatacagc agattggttt ttgaaacacc cgccaactct gcgatctgct
    18241 ccagccgtgt gccgtgaaaa ccgaattgtg aaaaagtgtc cagtgctgcg ctaagaatcg
    18301 cttttttctt cgcgcttact gcgcgcgaac gtttacccgt tgttttcact gcgccttgcg
    18361 tcatgcgctc tcccctcttg tagattggca acaggatagc aaaacccccg ccc
//
LOCUS       DAOFQD010000094        18403 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.094, whole genome shotgun sequence.
ACCESSION   DAOFQD010000094 DAOFQD010000000
VERSION     DAOFQD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.094"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..368)
                     /locus_tag="QBF46_003328"
     CDS             complement(<1..368)
                     /locus_tag="QBF46_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDQ6675476.1"
                     /translation="MNIKQPQYIRSALALAVCIGLSGPVLAQSAASPSAAAPSVAPKA
                     AQPQVDDKAAQEAEKKRSELTQDAITALTKTQEALTLLDANKTKEALAALELATGKLE
                     LVLARDAKLALAPVDVRVIT"
     gene            complement(390..848)
                     /gene="hsp20-GI"
                     /locus_tag="QBF46_003329"
     CDS             complement(390..848)
                     /gene="hsp20-GI"
                     /locus_tag="QBF46_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock protein sHSP20-GI"
                     /protein_id="HDQ6675477.1"
                     /translation="MSALTPWDPFRELDELQNRLATMLGRTPQRQGARTGNEALTTAD
                     WAPMVDISEDENAFLLKLDLPEVPKDAVRVSAENGVLTISGERKLEKEEQGKKFHRIE
                     RAYGRFVRSFVLPDNVDPTKVTASMKDGVLEVRLVKAEQAKPKQIEISVN"
     gene            complement(927..2756)
                     /gene="ftsH"
                     /locus_tag="QBF46_003330"
     CDS             complement(927..2756)
                     /gene="ftsH"
                     /locus_tag="QBF46_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDQ6675478.1"
                     /translation="MEKKDQWNIGYWIVAGLLLLTLQNYWQAAKTVEPVPYSEFEKAL
                     AEGRVAEVLVSDRTVTGHLKSPDSRGKTTIVATRVEPDLAERLSKYDVPYARVVESTW
                     LRDVLSWILPAVAFFGVWFFLFRRFAEKQGMGGFLSIGKSRAKVFMEKNTGVTFADVA
                     GVDEAKAELVEIVDFLKNPQEYGRLGARIPKGVLLVGPPGTGKTLLAKAVAGEAAVPF
                     FSISGSEFVEMFVGVGAARVRDLFEQARGQAPAIIFIDELDALGRARGVGGPIGGHDE
                     REQTLNQLLTEMDGFDSSVGLIILAATNRPEILDQALLRAGRFDRQVLVDRPDKKGRL
                     DILKVHVKKVTLAQDIDLEQVAALTTGFSGADLANLVNEAALAATRRKASAVELQDFT
                     AAIERIVAGLEKKNRVLNPKERETVAYHEMGHALVALALPGTDPVHKISIIPRGIGAL
                     GYTLQRPTEDRFLMTRADLEHKIAVLLGGRAAEKLVFGELSTGAADDLARATDIARDM
                     ITRFGMDEGLGYIAFEAQRPRFLDTPELAHGGCRVAESTQARIDQAIRDIVMGVFERA
                     YRILDTNRAVLERCARELLARETLDENDILQLTQGLVLDRNEK"
     gene            complement(2784..4214)
                     /gene="cls"
                     /locus_tag="QBF46_003331"
     CDS             complement(2784..4214)
                     /gene="cls"
                     /locus_tag="QBF46_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDQ6675479.1"
                     /translation="MSDPNGLTWAATLVSLAVAIPTAGHAVIYKRDPRSATLWVLLIA
                     LLPLGGSLLYGLFGINRYQRRARRLFPGADPAVRQDLSPTIPEAVSPPLAGLAHLVGR
                     ATGQSLTGGNRIEPLVDGEQAYPAMLAAIESARYSVALTSYIFDSQGIGAQFVDALRR
                     AHERGVQVRVLIDDVYARWTPRSAYRALQRAGVPAATFNPTLIPARLHAAHLRNHRKL
                     LVIDGETGFTGGLNIFSPYWRPDAPDQACHDLHFRLRGPVIAHLMRCFTDDWCDTTGE
                     RLSKGYWGEPPVTADEQGTSWARGIEAGPDEALDRMRWTFMGALSAAKHSVRIWTPYF
                     VPDQPMIAALSTAALRGVRIDVLTPANGDHPTVQWAARAHYWQVLEHGVRIFERPGPF
                     DHSKLMLVDGAWCCLGSANWDARSLRLNFEFNVEVYDTALSTRLSSLFDAARDASGEI
                     SAKALRARPLAIRLRDGVARLFTPIL"
     gene            complement(4231..7080)
                     /gene="clpK"
                     /locus_tag="QBF46_003332"
     CDS             complement(4231..7080)
                     /gene="clpK"
                     /locus_tag="QBF46_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock survival AAA family ATPase ClpK"
                     /protein_id="HDQ6675480.1"
                     /translation="MARKQCQVCGQPATVRVEANLNGRHSTMLLCDDHYRQLARQQKR
                     TVSPLEALFGSRSGLFEDFLGSDFFRIGDDSPSMAADTDEVVDASFGEPAPAGTGTAR
                     RRGSGLASRISEQSEALLQEAARHAAEFGRAEVDTEHLLLALSDSDVVKTILGQFKIK
                     VDDLKRQIESEAKRGEKPFEGEVGVSPRVKDALSRAFVASNELGHSYVGPEHFLIGLA
                     EEGEGLAANLLRRYGLTPQALRQQVSKVVGKGAEDGRAETPTNTPELDKYSRDLTKMA
                     REGKLDPVIGRAQEIETTIEVLARRKKNNPVLIGEPGVGKTAIVEGLAQRMVAGEVPE
                     TLRDKRLVELNINAMVAGAKYRGEFEERVQKVLKEVTEHQGEMILFIDEVHTIVGAGQ
                     GGGEGGLDVANVFKPMMARGELNLIGATTLNEYQKYIEKDAALERRFQPVMVPEPTVA
                     QTMMILRGLRDTFEAHHKVSITEDAIIAAAELSDRYITARFLPDKAIDLLDQAAARVK
                     LSATARPVAVQELESELHQLRREQDYMASRKQYDKAAELGKRIEAKETELKQLVEEWE
                     RERASGSAEVKAEHVAQIVSRLTGIPVNELTVEEREKLLHLEQRLHERLVGQDEAVRA
                     VADAVRLSRAGLREGDKPVATFLFLGPTGVGKTELAKALAESIYGDEGALLRIDMSEY
                     GERHAVARLVGAPPGYVGYDEGGQLTEKVRRKPYSVLLLDEIEKAHPDVYNILLQVFD
                     DGRLTDGKGRVVDFTNTIIIATSNLGSDIIQRRLKAGGAAGEEYEKTKSEVMDVLRGH
                     FRPEFINRIDEIIVFHALGKEEIRHIVGLQLDRVARNAASQGVTLTFDQTLIDHFAEE
                     GYKPEFGARELKRLIRSELETALAREMLGGGIGKGDHANARWDDKAERVVFERQEPPA
                     KSAEPEKPDAADVAEAPPGDASKPARKKKSADGES"
     gene            complement(7178..7747)
                     /locus_tag="QBF46_003333"
     CDS             complement(7178..7747)
                     /locus_tag="QBF46_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003847761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDQ6675481.1"
                     /translation="MDIDFKKLAPWNWFKKEQEEQQSTASLPVQRNDLPVAGGPVSPI
                     LQLHREIDRLFDDTFRGFGFPALAMPRWPSDWPGLLKPALDIQETDKQYKISLEVPGV
                     EEKDIHITLDNDVLLVRGEKRQEQESKDGGFHRVERSYGSFQRALNLPTDANQDTIKA
                     AFKNGVLTITMEKREASAPKQGRSIPING"
     gene            complement(7783..8064)
                     /locus_tag="QBF46_003334"
     CDS             complement(7783..8064)
                     /locus_tag="QBF46_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6675482.1"
                     /translation="MTARQQAIFTLDELATYLKVGKRTLYRLAAHGEIPAFKVGGTWR
                     FRQSEIDRWINDQIQAGRKKEVIRTDEQPKSAKHPVLPGRAIHSRRQTE"
     gene            complement(8292..8555)
                     /locus_tag="QBF46_003335"
     CDS             complement(8292..8555)
                     /locus_tag="QBF46_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675483.1"
                     /translation="MGHGLRRRCREGVLAGRILLNYVVWGNGGFSARLWNAIRSDDWA
                     IPHIGLSSLGEIVGWARPDEFPPRNMRTSKGLRALGYNVRIGV"
     gene            8570..8833
                     /locus_tag="QBF46_003336"
     CDS             8570..8833
                     /locus_tag="QBF46_003336"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6675484.1"
                     /translation="MPPQRRTYSKSFKAQVIQECSEPGASIANIALGYSLNANLVHKW
                     IRLHTQKTTAIQPAFIPLPSQMLGAGSHAQVRCCDHEFDPVGP"
     gene            8790..8942
                     /locus_tag="QBF46_003337"
                     /pseudo
     CDS             8790..8942
                     /locus_tag="QBF46_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(9233..9334)
                     /locus_tag="QBF46_003338"
                     /pseudo
     CDS             complement(9233..9334)
                     /locus_tag="QBF46_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(9426..11048)
                     /locus_tag="QBF46_003339"
     CDS             complement(9426..11048)
                     /locus_tag="QBF46_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6675485.1"
                     /translation="MNRTVFRQIPNGSRLLAVGNFFRAKTPAAQWQLAALFERNGQLQ
                     LQRFAQEMSCVLAVGREFLGEEGAPYRPSGFKKTVVLPAIDTWRERQLGECPRLARRL
                     AANPEISGQRCFVFEVDGLTVWLPKFELARKMFFHAAFIVRAAFAPNGLDMAFTIYRE
                     GDVVHIHTPPKTGAPSQLLKIKGYRDHFSWLLLNQDVRRSFESIWQSLNQRQERVLQE
                     SAYARWQFDFQAPTSLAGTTMDVRGPFDPESNELLVWEIEKLQGLRFSHRGDIFFHHP
                     ALKLPVRGENTGCVSVPSGAKELEVDADEEPTAAKEQRLIHLPVEGVTFDAQLRTKIA
                     YNGERANGHGKKVDDEQCPSRESQLFGVADPVSEGSIASGEFQQLGEGEGDGDEHERL
                     ADRFSLLREILEQITQESGVALVALEVRPLPPVPRCSYQMIDHEKSRCYLLVRFKLQN
                     GDQRYLLEIDTSDNRKTMSTRIMGFKAGVEAGKCIDRILRGTVKGSLRWPGTMAKYCE
                     PLHSVHHPKESSSGANHARVFDWKQRIRAALG"
     gene            complement(11041..12543)
                     /locus_tag="QBF46_003340"
     CDS             complement(11041..12543)
                     /locus_tag="QBF46_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDQ6675486.1"
                     /translation="MYFPRLYPDELLYSGIARCRVHLGIGSHKTLLHMLFGDSKVAAI
                     TDLPTHLTALANNTGLDAEGMILNHTLFPLYAPFIPPERRTDLFQAMLAPDRPTIGLS
                     GASTALVKWPDWLRYCPICFEDMAARYGEPYWRRSWQIQGIDACPEHGCQLLNSPIPF
                     RRAQRHEFHTASPLFLPRDLRVSPVGEEAIRLAKAAMQLLALEEVQSPGYGRWTNLYR
                     YLATECGARRGRQVRAEVIWEKILASHQRDWLAANGLLTSGEPPPWLLAMFRKHRKGF
                     SALQHLIVWTSLRPGQHAGELISEAKTRQVDLASEWSVQLLPAEFEQKQQYRAIWLQA
                     LANHGGAKAARQDGAGACYAWLYRHDRHWLMVANQVRQRRQGNNSHIDWRARDIRLVR
                     LLIRIGRGSEEDLGLPRRSRNWFLQQLPHRASVEHRLDQLPLCCTFLDRYAESVGEYQ
                     IRRLTAAMLKDVQTGITSRRWELEKRCGLEKSRVAPLTTAFIRLIGRWIE"
     gene            complement(12545..14155)
                     /locus_tag="QBF46_003341"
     CDS             complement(12545..14155)
                     /locus_tag="QBF46_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017781028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ6675487.1"
                     /translation="MTEEFQRGVIPLARYQEQQLPEYQDNPLISALPPIPDLQEVVDL
                     LQQLPGFEPQEALLDGRLRAHAIARLLHGFFQPLSHHLELESKISLMIRQGYIGRNPA
                     SGAWYAHLQNGYRRVEEEDLDAAVYQSVSSTANSLSLFGCSGCGKTRTLERILGMYPQ
                     ALHHPDYDITQLVYLKIDCPIDGDLDELCLSFFNEVDKILGTHYSRSHGRKKLGTKRL
                     LASMCQLANLHTLGVLIIDEVQNLNEARSGGAEKMHNFFVSLVNTIGVPIIQVGTHRA
                     SKFFQRTFRAARRVSGLGSIRWDRLPRDEHWKRLLKRLWKYQWLRNAAPLDDELEATM
                     YDLTQGVMDIVIKLFALAQIRAIVTGVECIKPLLLRRVFEDEFKPVHPMLAALRSGRP
                     ELIAKYDDLLMPEIEGRLLTLTRSLDSVPQPKPPAPPADDKAKRLAALLEGMEIPRDI
                     AIPMADELLAENPDIPLAALIHKATAYLAQDAPKRPGISRVKRTEWGCLSEEDLRRRY
                     ADGPESEAYDRFKQAGLILDLRPLLRAS"
     gene            complement(14148..16208)
                     /locus_tag="QBF46_003342"
     CDS             complement(14148..16208)
                     /locus_tag="QBF46_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017781027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6675488.1"
                     /translation="MGELRKRLLWSGTEQAIWIDIDSDTALPEPISIVELERLIMERE
                     LESIADPFEETVLREVEEGSLDQQKRDEAWGMLADFVHNPQLFVRRPRGLIVRGIMER
                     HGVTNQTVYRLLRRYWQRGMCRNALLPDYVNSGARGKRRKPNQAKLGRPRVVMEGKGS
                     NVTPDIERIFRRVIEERLLKEKHPSIPDAYAAGLNLLRAVLTELPTSELPTLGQFRYF
                     YGREYHFTDTLPCRVSAVDFAKDFRPLSSTSTTEALGPGYRYQIDATIADIYLVSEHD
                     RSLIVGRPVVYMVIDVFSRMVVGMYVGFEGPSWVSAMVALANTVADKVEYCRQYSVEI
                     DASDWPVKGLPDVVLADKGELNGTKVEAFSQAFGVRIENAPARRGDAKGIVERYFRTV
                     QERFKPYASGVVEDTTSRKRGGHDYRLDASLTLPEFTQIIIAGILYHNNFHTLSKYDR
                     TSGMPGDLPAIPVMLWNWGLANLTGRLRTAPEELVRINLLPHESATVSELGIRLFGCF
                     YTCQEAIREGWFHRGQGRRPTGVTVAYDPRSADHIYLRPSNSLKDYWVCDLADRSRRF
                     RGMTFWDVWLLSREERRSDTNAAAEALVERGNLLKQIESIVAQAEDASPLKTGMTTKD
                     LGTQIRENKQQEKRQERQKTAFKPDKAQQEKPAEVIPLRGEKQEDYAFPDLSDLIFKE
                     EEDD"
     gene            complement(16222..17049)
                     /locus_tag="QBF46_003343"
     CDS             complement(16222..17049)
                     /locus_tag="QBF46_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HDQ6675489.1"
                     /translation="MSGTFKGLTQAQIDRRLKDGRGQGQGSDYKPFIYTRDVSSLGRS
                     HRLPGSKTRRLHHLLSDLELAIFLTLDRSPHVTDIREQFPMRVEDTVRIAEELGLPHG
                     CYKGTPQVLTSDFLVDFEDPQRPSIAIQAKYSTDLQKPEVIERLELERRYWQEKGIPW
                     VIVTEREVSKVAFANIQWLYPAHAEGDIAQDDLAHYQQLFLLEFQRHPDRKLTAIAQG
                     LDTSGQLEAGQALYWLRQLLARHCFLFDLDTPYRELKPKDLVANSHQMHQELSSVAR"
     gene            17146..17964
                     /locus_tag="QBF46_003344"
     CDS             17146..17964
                     /locus_tag="QBF46_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HDQ6675490.1"
                     /translation="MNLYLHPTRVGETTAKVEQAAIETFIARAKSPCLGGYPWLENNE
                     QPKGTVAVYPEKIGYVEYIDMVKLGSLLSNDPRHVYLVAQPGSFIHPSMPVLYLSSGQ
                     ESSICTDLLETIIVSDVRSFAQDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVR
                     ILSAYAQNKIDETEVIYPSVHVAPLQIKDLLEDFFLPVARDGASMREIQIRVLKGLSM
                     LSKGWPGMFAEAAHILASETLEHATRTGHIAHDEYLIKSIYYNLFSGENPNKKP"
     gene            18218..18403
                     /locus_tag="QBF46_003345"
                     /pseudo
     CDS             18218..18403
                     /locus_tag="QBF46_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT         3565 a         5694 c         5316 g         3828 t
ORIGIN      
        1 tgggtgatga cgcgtacatc gaccggcgcc aaagcaagtt tggcgtcgcg tgccaatacc
       61 agttccagct ttccggtggc cagttccagc gcagcgagcg cctccttggt cttatttgca
      121 tcaaggaggg tcaaagcctc ctgggtcttg gtgagcgccg tgatggcgtc ctgagtgagc
      181 tcggaacgct ttttttcggc ttcctgggcg gccttgtcgt ctacttgtgg ctgcgccgcc
      241 ttgggtgcga cagagggcgc tgcagcgctt ggacttgccg ccgattgggc caaaaccggt
      301 ccactcagtc cgatgcagac cgccagggca agtgcggaac ggatgtattg gggctgtttg
      361 atattcatga tgttggactc caattgaagt taattgactg aaatttcaat ctgcttcggc
      421 ttggcttgct cggccttgac aagccgcact tccagcacgc cgtctttcat cgaagccgtc
      481 accttggtcg gatcaacgtt gtcaggtaag acaaagctgc gcacaaagcg gccatacgca
      541 cgttcgatgc ggtggaattt cttgccctgc tcctcttttt ccagtttgcg ctcgccgctg
      601 atggtgagca cgccgttttc cgcgctgacg cgcacggcat ccttggggac ctccggcaga
      661 tccagcttga ggaggaatgc gttctcatcc tcgctgatgt ccaccattgg tgcccagtcc
      721 gccgtggtca gggcttcgtt gccggtacgg gcgccctgtc gctggggtgt ccgtcccaac
      781 atcgtcgcca ggcggttttg caattcatcc agttcccgga aggggtccca cggagtcaat
      841 gcagacatat cggttctcct tgaacaatgc cggcgaattg gatgacgcac cgaacagagc
      901 cgccggctca ctctgaatag cgcctactac ttttcgtttc tatctagaac aagtccttgg
      961 gtcaattgaa ggatgtcgtt ctcgtcgagc gtttcccgcg ccagcagttc gcgcgcacaa
     1021 cgctccagca ccgcgcggtt ggtgtcgagg atccggtagg cgcgctcgaa cacgcccatc
     1081 acgatgtcgc ggatggcctg atcgatgcgc gcctgggtcg attcggccac ccggcagccg
     1141 ccgtgggcca gttccggtgt atcgagaaag cgtggccgct gcgcctcgaa ggcgatgtag
     1201 cccagtcctt cgtccatgcc aaagcgggtg atcatgtcgc gggcaatgtc ggtggcccgt
     1261 gcgaggtcat cggcagcccc ggtggacagc tcgccgaaca cgagcttctc ggccgcacga
     1321 ccgcccagca gcacggcgat cttgtgttcc agatcggcgc gcgtcatcag gaagcggtct
     1381 tcggtggggc gttgcagcgt gtagcccagc gcgccgatgc cgcgcgggat gatcgagatc
     1441 ttgtgcacgg ggtcggtgcc gggcagcgcc agcgccacca gcgcatggcc catctcgtga
     1501 taggccacgg tttcccgctc cttgggattg agcacgcggt tcttcttctc caggcccgcc
     1561 acgatgcgct cgatggcggc ggtgaaatcc tgcaactcca cggcggacgc tttgcgccgg
     1621 gtcgcggcca gcgcggcctc gttgaccagg ttcgcgaggt ctgcacccga aaagcccgtg
     1681 gtcagcgcag ccacctgttc gagatcgata tcctgagcca gcgtcacctt cttgacgtgg
     1741 actttcagga tgtccagccg tcccttcttg tcgggccggt ccaccagcac ctgacggtcg
     1801 aagcgaccgg cgcgcagcag cgcctggtcg aggatttcgg ggcggttggt ggcggcgagg
     1861 atgatcagcc cgaccgagct gtcgaagccg tccatctccg tgagcagctg gttgagggtc
     1921 tgctcgcgct cgtcgtggcc gccgatgggg ccgccgacgc cgcgcgcgcg gcccagcgca
     1981 tcgagctcgt cgatgaagat gatggccggc gcctgcccgc gggcctgctc gaacaggtcg
     2041 cgcacgcgcg ctgcacccac gccgacgaac atctcgacaa actctgatcc ggagatggag
     2101 aagaagggca ccgccgcctc gccggcaaca gccttggcca gcaatgtctt gccggtgccc
     2161 ggtgggccga ccagcaacac gcccttggga atgcgcgccc cgagccgtcc atactcctgc
     2221 ggattcttga ggaaatcgac gatctcaacc aactccgcct tggcttcatc gacgccagcg
     2281 acatcggcaa aggtcacgcc agtgttcttc tccatgaata ccttggcacg gctcttgccg
     2341 atgctcagga agccacccat gccctgcttc tcggcgaagc ggcggaacag gaagaaccag
     2401 acgccgaaga aggccaccgc cggcagaatc caggagagca catcacgcag ccaggtgctt
     2461 tccaccaccc gcgcataggg cacgtcgtac ttggacagcc gctcggccag gtcgggttcg
     2521 acacgggtgg ccacgatggt ggtcttgccc cggctgtccg gcgatttcag gtgcccggtg
     2581 accgtgcggt ccgacaccag cacttcggcg acgcgcccct cggccagcgc cttctcgaat
     2641 tcgctgtagg gcacgggctc gacggtcttg gccgcctgcc agtagttctg cagcgtcagc
     2701 agcaacaggc cggcgacgat ccagtagcct atgttccatt gatctttctt ttccatgggc
     2761 aatgttcctc gcaagtcgtg tcgctagaga atgggggtga acagacgggc caccccgtcg
     2821 cgcaagcgga tggccagcgg gcgggcgcgc aacgccttcg ccgatatctc gcccgacgca
     2881 tcacgagcgg catcaaaaag ggatgacagc cgcgtagaca acgcggtgtc gtacacctcc
     2941 acattgaact cgaagttgag gcgcaaactg cgtgcatccc aattggccga acccaggcag
     3001 caccacgctc cgtctaccag catcagcttg ctgtggtcga acgggccagg ccgttcgaag
     3061 atgcgcacac cgtgctccag cacctgccag tagtgggcgc gcgcggccca ttgcaccgtg
     3121 ggatggtcgc cgtttgcggg cgtcagcacg tcgatacgca cgccgcgcaa cgcggccgtg
     3181 cttagcgccg cgatcatcgg ctgatcgggg acgaagtaag gtgtccagat gcgcaccgaa
     3241 tgcttcgcgg cgctcaaagc gcccatgaag gtccagcgca tccggtccag ggcctcatcg
     3301 ggaccggcct cgatgccgcg cgcccaagag gttccttgct catcagccgt tacgggcggt
     3361 tcgccccagt aacccttgct gagccgctcg cccgtggtat cgcaccaatc atcggtgaag
     3421 caccgcatca gatgcgcaat caccggcccg cgcagacgaa agtgcaaatc gtgacaggcc
     3481 tggtccggcg catcgggccg ccagtacggg ctgaagatgt tcaatccgcc ggtgaaaccc
     3541 gtctcgccat cgatcaccag cagcttgcgg tgattgcgca gatgcgcggc atgcaggcgc
     3601 gcgggaatca atgtagggtt gaacgtcgcc gccggaacgc cggcgcgctg caaggcgcgg
     3661 taggcgctgc ggggcgtcca gcgggcatac acgtcgtcga tcagcacccg cacttgcacg
     3721 ccgcgctcat gagcccggcg cagcgcatcg acgaactgcg ccccgatacc ttggctgtcg
     3781 aagatgtacg aggtcaaggc gacgctgtac cgcgccgatt cgatggcggc gagcatggcc
     3841 gggtaggcct gctcgccatc gacaagcggc tcgatgcggt tgccgccggt cagcgactgg
     3901 ccggtggcgc gtcccaccag gtgggccaga ccggcaagtg gcggcgatac agcctcgggt
     3961 atcgtgggcg agagatcctg ccgaacagca ggatctgccc ccgggaagag ccgccgcgcc
     4021 cgccgctggt aacggttgat gccgaacagc ccatacagca gcgaaccgcc cagcggcagc
     4081 aaggcgatca gcagtaccca cagcgtggcc gatcgagggt cacgcttgta gatgaccgca
     4141 tgccccgctg tggggatagc gaccgccagc gacacgaggg tggctgccca tgtcagccca
     4201 ttggggtcgg acacgacagc ctcccatcgc tcaagattcg ccatccgctg acttcttctt
     4261 gcgcgcaggc ttgctcgcgt cgccaggcgg tgcctcggcc acgtccgcgg catcgggctt
     4321 ttcaggctca gccgacttcg cgggtggctc ctggcgctcg aagaccaccc gttcggcctt
     4381 gtcgtcccag cgggcgttgg cgtgatcacc cttgccgata ccgccaccca gcatctcgcg
     4441 cgccagcgcg gtttccagct cgctgcggat cagccgcttg agctcacgcg cgccgaactc
     4501 gggcttgtag ccttcctccg cgaagtggtc gatcaaggtc tgatcgaagg tcagcgtcac
     4561 gccctggctg gcggcgttgc gggccacacg atcaagctgc aggccgacga tatggcggat
     4621 ctcctccttg cccagcgcat ggaagacgat gatctcgtcg atgcggttga tgaactcggg
     4681 gcggaagtgt ccgcgcagca cgtccatcac ctcggacttg gtcttctcgt attcctcgcc
     4741 ggcggcgcca ccggccttca gccgacgctg gatgatgtcc gagcccaggt tcgaggtggc
     4801 gatgatgatg gtattggtga aatccaccac ccggcccttg ccgtcggtga gccgcccgtc
     4861 gtcgaacacc tgcagcagga tgttgtagac gtcggggtgc gctttttcga tctcatcgag
     4921 cagcaacacg ctgtagggct tgcgacgcac cttctcggtg agctggccgc cctcgtcata
     4981 gcccacataa cccggaggcg cgcccaccag gcgtgccacg gcatggcgtt ccccgtactc
     5041 ggacatgtcg atgcgcagca gcgcaccttc atcgccatag atggactcgg ccagcgcctt
     5101 ggcgagctcg gtcttgccca cgccggtcgg cccaaggaac aggaaagtcg ccaccggctt
     5161 gtcgccttcg cgcaggcccg cgcgcgacaa ccgcacggca tcggccaccg cgcgcaccgc
     5221 ttcgtcctgg cccaccaggc gctcatgcag ccgctgctcc agatgcagca gcttctcgcg
     5281 ttcttccacc gtcagctcgt tgaccggaat gccggtcagg cgcgagacga tctgcgcgac
     5341 atgctcggcc ttgacttcgg cgctgcccga ggcgcgctcg cgttcccatt cctcgacgag
     5401 ctgcttgagt tcagtctcct tggcctcgat gcgcttgccc agctcggcgg ccttgtcgta
     5461 ctgcttgcgc gaggccatgt agtcctgctc acgccgcaac tggtgcagtt cggactccag
     5521 ctcctgcacc gccaccgggc gagccgtggc cgacagcttc acgcgtgcgg ccgcctggtc
     5581 aagcaggtcg atggccttgt caggcaaaaa gcgcgcggtg atgtagcggt ccgacaactc
     5641 ggcggcggcg atgatcgcat cctcggtgat gctgaccttg tggtgcgcct cgaaggtgtc
     5701 gcgcaggccg cgcaggatca tcatggtctg cgctaccgtc ggctcgggca ccatcaccgg
     5761 ctggaaacga cgctccagcg cggcgtcctt ctcgatgtac ttctgatact cgttgagcgt
     5821 ggtggcgccg atcaggttca gttcgccgcg cgccatcatc ggcttgaaca cgttggccac
     5881 gtccagcccg ccttcgccgc caccctggcc tgcaccgacg atggtgtgca cttcatcgat
     5941 gaagagaatc atctcgccct ggtgctcggt cacttccttg agcaccttct gcacgcgctc
     6001 ctcgaactcg ccgcgatact tggcgcctgc caccatggcg ttgatgttga gctcgaccag
     6061 acgcttgtcg cgcagcgtct cgggcacttc accggcgacc atgcgctgcg ccagcccttc
     6121 gacaatggcg gtcttgccga cgccgggctc accgatcaac accgggttgt ttttcttgcg
     6181 ccgggccagc acctcgatgg tggtctcgat ctcctgcgca cggccgatga ctggatcgag
     6241 tttgccctcg cgcgccatct tggtgaggtc gcgcgagtat ttatccagct cgggcgtgtt
     6301 ggtcggcgtc tcggcgcggc catcctcggc ccctttgccg accaccttgc ttacctgctg
     6361 gcgcagcgct tgcggcgtga ggccgtaacg gcgcagcagg ttggccgcca aaccttcgcc
     6421 ttcctcagcg agcccgatca ggaaatgctc cggcccgaca taagagtggc cgagttcgtt
     6481 ggaggccacg aaagcgcggc tgagcgcgtc cttgacccgg ggcgacacgc cgacttcgcc
     6541 ctcgaacggc ttctcgccgc gcttggcttc ggattcgatc tgtcgcttga ggtcatcgac
     6601 cttgatcttg aactgtccca ggatggtctt caccacgtcg ctgtcggata gcgccagcag
     6661 caggtgttcg gtatcgactt cggcgcgccc gaactctgca gcgtgtcggg cggcctcctg
     6721 caatagggcc tcggactgtt cgctgatacg gctggcgagc ccactgccgc gacggcgcgc
     6781 agtgcccgta ccggccgggg cgggttcgcc gaacgaggca tcgaccacct cgtcggtatc
     6841 ggccgccatg gacggcgaat cgtcaccgat gcggaagaag tcgctgccga ggaagtcttc
     6901 gaacaagccg ctgcgcgagc caaacaaggc ttccagcggc gagacggtgc gcttttgctg
     6961 gcgcgccagt tggcgatagt gatcgtcaca caacagcatg gtgctgtggc gaccattgag
     7021 attggcttcc acccgcaccg tggcgggctg gccgcagacc tggcattgtt ttctggccat
     7081 gctcatgctc cttgagagga ctgatttgac gaggcgccga aggtcacggc gcctcgccat
     7141 tcacttggtt tttctgtaga gaacaagccg atcggcgtca gccgttgatc gggatcgagc
     7201 ggccctgctt gggcgcactg gcctcgcgct tttccattgt gatcgtgagt acgccgttct
     7261 tgaaagcggc cttgatcgtg tcctggttgg catcggtcgg caggttcaga gcgcgctgaa
     7321 agctgccgta ggagcgctcc acccggtgga aaccgccgtc tttgctttcc tgctcctgac
     7381 gcttttcacc gcgaaccagc agcacgtcgt tgtcgagcgt gatatggatg tctttttcct
     7441 cgacgccggg aacctccaaa gaaatcttgt actgcttgtc ggtctcctgg atgtccagcg
     7501 ccggcttcag caggcccggc cagtccgacg gccagcgtgg catggccagc gccggaaagc
     7561 cgaagcctcg aaacgtgtcg tcgaagaggc ggtcgatctc gcggtgcaat tgcaggatgg
     7621 gactgacggg cccacccgct accggcaggt cattgcgctg caccggcagc gaggcagtgc
     7681 tctgttgttc ttcctgctcc ttcttgaacc agttccaggg agccaacttc ttgaaatcaa
     7741 tgtccatgtc acacctccag aacaaaaatt gaagactctc gctcactccg tttgcctgcg
     7801 gctatggata gcgcgcccag gcaacacggg atgtttcgct gacttcggct gctcatctgt
     7861 acgtatcacc tccttctttc tgcctgcttg gatctgatca ttgatccatc gatcgatttc
     7921 actttgacga aaccgccacg tcccgccgac cttgaaggcc ggaatctccc cgtgcgcggc
     7981 gagccggtag agcgtccgct tgccgacctt taagtaggta gccaactcgt cgagtgtgaa
     8041 gatcgcctgc tggcgcgctg tcataccacc cccacaagtt ccgtcgtcac gcggcctcgc
     8101 ctgaggaagt tcttgcaaga ctttgcaaga tattgcgcct aatatgccaa aagtcaagag
     8161 tcaacatcca gcgatctgct ggccgattga tgtgactcaa atcttcaagg acggtatgcc
     8221 agggcgggcc ggtccggcgc aggggacggt gggttgatcg ggaccttgcg ggcgccgccc
     8281 acggccgcag atcaaacacc gatcctcacg ttgtaaccga gcgcccgcag ccccttgctc
     8341 gtgcgcatgt tccttggcgg aaactcgtct gggcgcgccc agccgacgat ttccccgagg
     8401 ctgctcaggc caatgtgcgg aattgcccag tcgtcgctgc ggatcgcgtt ccagagtcgg
     8461 gccgaaaaac ctccgttgcc ccagacaacg tagttcaaca gtatccgtcc tgccaacaca
     8521 ccttcccgac accgccgccg aaggccatgg cccatcaagc ggattttcca tgccccctca
     8581 gcgccgtacc tacagcaaat ctttcaaggc ccaggtcatt caggagtgtt ctgaacccgg
     8641 tgcctccatc gcaaacatcg ccttgggcta cagcctcaat gccaacctcg tccacaaatg
     8701 gattcggctg catacccaga aaaccacggc tatccagcca gcgttcatcc cgttgccttc
     8761 tcaaatgctc ggcgcgggat cgcatgctca agtgcgctgc tgcgatcatg agtttgatcc
     8821 agtcggcccg taataatggg cacgatccgt atgcctatct caaggatgtg ctgacacggt
     8881 tgccgacgca gcgggcgagt gagattgcag agctgctgcc gcataggtgg catccggcat
     8941 agtcgcgcaa gacggtctgc ccgcacgctt acgttcaaca gctcaagcac cgtccgccct
     9001 tcgcgggatc gttgccgcca gagccacacg ccgaactttt ccaccttgtc gtcgccggcc
     9061 tgcggtcagt caggcaagcc caggtgcttg ttttcctgct tgatcgcgtg gtctcggatc
     9121 gcgtgcaccc gggaaacggc atccacgaac tgccaccatc tcttagcttg gcggggtcaa
     9181 gccagccggg atgcccggac ggttacgctg gtccgcgccc agttgcagca ccaaggtcgg
     9241 ccgcagtgat gaagcgggtc ttgatgccgc actgcgccgc ccgatagcgc agtgcgatgg
     9301 ccagatgcgt cttgccgact cccaaggggc ctatatcgcc ttggcacggc tccgggcagt
     9361 ttcggggagg ctgcacgcag tgcgcgacaa gatgtatctc agccgaactg cggtgtattc
     9421 tgcaatcagc ctagggccgc gcgtatccgc tgcttccagt cgaacacccg ggcatggttg
     9481 gccccagatg agctttcttt cgggtgatgc acagagtgca aaggctcaca gtacttcgcc
     9541 atggtgcctg gccaacgcaa cgagcctttc actgttcccc tcaagatcct gtctatgcat
     9601 tttcctgcct caacacctgc tttgaagccc atgatccgcg ttgacatggt cttccggttg
     9661 tctgaggtat ctatttctag aaggtagcgt tgatcgccgt tttgcagctt gaaccttacc
     9721 agcagatagc agcgtgattt ttcgtgatca atcatctggt agctgcaacg tggaacgggc
     9781 ggcagaggcc tcacttccag tgctaccagc gcaactccag actcttgggt gatttgttca
     9841 agaatctctc ggagcagcga gaagcgatct gcaagccgtt catgctcgtc cccatcaccc
     9901 tctccctcac ccaattgttg gaattctcct gacgctatgc tgccctcgct cacaggatca
     9961 gccacgccga agagttggct ttctctgctc gggcattgct cgtcgtcgac cttcttgcca
    10021 tgaccgttgg cacgctcccc gttgtaggcg atcttggtgc gcagctgagc atcaaacgtg
    10081 actccctcaa cgggcagatg aatcaacctc tgctccttgg cagcagtcgg ctcttcgtca
    10141 gcatccacct ccaactcttt cgctcccgat ggcacggata cacagcctgt gttctctccc
    10201 ctgacgggta acttgagagc agggtgatgg aaaaaaatat caccgcgatg gctaaaccgg
    10261 agcccctgaa gcttttcgat ctcccagacc agcaattcgt tgctttccgg atcaaagggg
    10321 cctcgcacat ccatggtggt acccgcgagg gaagtgggtg cctgaaagtc gaactgccac
    10381 ctggcatacg ccgactcttg cagtacccgc tcttgccttt gattgaggct ttgccagatt
    10441 gactcgaagg atcgcctcac atcctgattc agcagcagcc aactgaaatg gtctctgtat
    10501 cccttgattt tgagaagctg acttggggcc cccgtcttcg ggggcgtatg gatatgaaca
    10561 acatcgccct ctctgtagat cgtgaaggcc atgtccaagc cattcggtgc gaaggctgca
    10621 cgaacgatga aggctgcgtg gaagaacatt tttcgtgcca gctcgaattt cggcagccag
    10681 acggtcagac catcgacctc gaacacaaag catcgctgtc cagatatctc aggatttgca
    10741 gcaagccttc tggccagacg tgggcactca cccaactgcc tttcccgcca ggtatctatt
    10801 gcaggaagca ccaccgtctt cttgaaacca ctgggacgat aaggagcccc ttcttcgcca
    10861 agaaactcgc gaccaacggc caagacacac gacatctcct gtgcgaaccg ctgcagctgc
    10921 agctgaccgt tacgctcaaa gagagccgcc aactgccact gggccgccgg agtcttggcc
    10981 ctgaaaaaat ttccaacggc cagcaacctg gatccgttcg ggatctgacg aaatactgtc
    11041 ctattcaatc catcttccta tcaatcgaat gaacgcagtg gtcagtggtg ccaccctgct
    11101 tttttccaat ccacaacgct tttccagctc ccagcgccta gacgttatgc ctgtctgtac
    11161 atccttcagc atcgctgcag tcaggcgacg gatctgatac tcgccgacgg actcggcata
    11221 gcgatccagg aaggtgcagc aaagaggcaa ttgatcgaga cggtgctcga ctgatgcccg
    11281 atggggtagc tgctgcagga accagttgcg actcctgcgc ggaaggccca ggtcttcttc
    11341 gctacctcta ccaatccgga tcagcaggcg caccaacctt atgtcacgcg ctcgccagtc
    11401 gatgtggctg ttgttgccct gtcggcgctg cctgacctgg ttcgcaacca tcagccagtg
    11461 tcggtcatga cgatagagcc aggcgtaaca tgcccctgct ccgtcttgcc tggctgcctt
    11521 ggctccaccg tgattagcca gtgcttgcag ccaaatggct cggtactgct gcttttgctc
    11581 aaactcagca gggagcagct gcactgacca ctcggaggca aggtcgacct ggcgagtctt
    11641 cgcctcgctg atcagctcgc cagcatgctg gccagggcgc aggctggtcc agacgatcag
    11701 atgctgcagc gcactgaatc ccttgcgatg cttcctgaac atcgccaaca gccaaggtgg
    11761 aggctctccg gatgtcagca gaccgttcgc agcaagccaa tccctttgat gggatgccag
    11821 gatcttctcc cagatgactt cagccctgac ctgccggcct cgcctggcgc cgcactctgt
    11881 agccaggtac cggtacaggt tggtccagcg tccgtagcca ggcgattgca cctcttcgag
    11941 cgccagcagc tgcatagcag ccttggcgag cctgatagcc tcttcgccca cgggactcac
    12001 gcgaagatca cggggcagaa acagtggaga ggctgtatga aactcgtgcc gctgcgctcg
    12061 acggaacggg ataggtgagt tcaggagctg acagccatgc tcgggacagg catctatccc
    12121 ttgaatctgc cagctacgcc gccagtaggg ttcaccgtag cgcgcagcca tatcttcaaa
    12181 gcagatcggg cagtaacgca gccaatcggg ccacttgacc aaggccgtcg aggccccaga
    12241 cagaccaatc gtcggccggt cgggagcaag catggcctga aaaagatcgg tacggcgctc
    12301 cggcggaatg aaaggggcat agagcgggaa cagcgtgtga ttcaggatca tgccttccgc
    12361 atccaagccc gtgttgttcg ccaacgcagt caggtgtgtg ggcagatcgg tgatcgcggc
    12421 aaccttgctg tcaccgaaaa gcatgtgcag caaggtcttg tggctgccta tccccagatg
    12481 tacccggcac cgggcaatac cgctgtaaag cagttcgtcc gggtagagcc tggggaagta
    12541 catgtcagct cgctctcagc agaggccgta gatcgaggat caagccagcc tgcttgaatc
    12601 tgtcataggc ctcgctttca ggcccatcgg cataacgtcg gcgcagatcc tcctcggata
    12661 ggcaccccca ctccgtccgc ttaactctgg aaatgccggg cctcttggga gcgtcctgcg
    12721 cgaggtaggc cgtggccttg tgaatcaggg ccgccagcgg gatgtccgga ttctccgcca
    12781 acaattcatc ggccatggga atggcgatgt ccctcgggat ttccatacct tcgagcaggg
    12841 cggccaaccg ctttgccttg tcatcagccg gaggggccgg tggtttaggt tgggggacgc
    12901 tatccagaga gcgagtcagg gtcagcaacc gtccctcgat ctctggcatc agcaggtcgt
    12961 catatttggc gatcagctcg ggcctaccgc tccgcaaggc cgccagcatc ggatggaccg
    13021 gtttgaactc gtcctcgaaa accctgcgca gcaacagcgg cttaatgcac tcgacacctg
    13081 tcactatcgc ccggatctgc gccagcgcga acagcttgat gacgatgtcc atgacccctt
    13141 gggtgaggtc gtacatcgta gcctccagtt catcatccag cggcgccgca tttcgcagcc
    13201 actgatactt ccaaagccgc ttcaacagac gcttccaatg ctcgtcccga ggcaacctat
    13261 cccagcggat gctgcccaac ccggagaccc gccgagcagc ccggaacgtg cgctggaaga
    13321 acttgctggc ccggtgggtg ccgacctgaa tgatcggaac accgatggtg ttcaccagcg
    13381 acacgaagaa attgtgcatt ttttcggcac caccggagcg tgcctcgttg aggttctgaa
    13441 cctcgtcgat gatcagcact ccgagcgtgt gcagattggc cagctggcac atggatgcca
    13501 gcagacgttt ggtacccagc ttcttgcgtc cgtggctacg gctgtagtgg gtacccagga
    13561 tcttgtctac ttcgttgaag aagctcaggc acagctcatc caagtcaccg tcaattgggc
    13621 agtcgatctt caggtagacc agttgggtga tgtcgtaatc agggtgatgg agagcttggg
    13681 ggtacatgcc cagaatccgc tccagcgtgc gcgtcttgcc gcaaccggag cagccgaaca
    13741 gcgacaggct gttggccgta gaggagacac tctggtacac cgccgcatcc agatcctcct
    13801 cctcgacacg gcgatagcca ttctgcaggt gcgcatacca ggcaccgctg gccggattcc
    13861 gaccgatgta cccttgacgg atcatcaggc tgatcttgct ctccagctcc aagtgatggc
    13921 tcagcggctg gaagaagccg tgcaacaggc gggcaatcgc atgtgccctg agccgaccat
    13981 ccagaagcgc ctcctgcggc tcgaaaccgg gaagctgctg caatagatcg accacctcct
    14041 gcagatcagg aatcggaggc aatgcgctga tgagcggatt gtcctgatac tcgggaagct
    14101 gctgctcctg gtaacgcgcc aggggaatga ctccgcgctg gaattcttca gtcatcctct
    14161 tcctccttga atatcaggtc actgagatct gggaaggcat aatcttcctg cttctcgccc
    14221 cgcagtggga tcacctcggc tggcttttcc tgctgtgctt tatccggttt gaatgctgtt
    14281 ttctggcgtt cctggcgctt ttcctgctgc ttgttctcgc ggatctgggt tcccagatcc
    14341 ttcgtggtca tacccgtttt gagcggactg gcatcctctg cctgggcaac gatggactcg
    14401 atctgcttca gaagattgcc tctctctacc aaagcttccg ccgccgcgtt ggtatcgcta
    14461 cgccgttcct ctctggacag cagccagaca tcccagaagg tcatccccct gaagcgtcga
    14521 ctgcggtcgg caagatcgca aacccagtag tccttgagac tattggacgg ccgcaggtag
    14581 atgtggtcgg cgctacgcgg atcgtatgcg accgttaccc ctgtcggccg gcgcccctgg
    14641 cctcggtgaa accaaccctc cctgattgct tcctggcagg tgtagaagca gccgaacagt
    14701 ctgattccca gctccgaaac cgtggccgac tcatggggca agagatttat ccgcaccagc
    14761 tcctcgggcg cagtacgtaa ccggccggtc aagttggcca agccccagtt ccacagcatg
    14821 accgggatgg ctggcagatc gcccggcata ccagaggttc tgtcgtattt gctcaaggta
    14881 tgaaagttgt tgtggtagag gatgccggca atgatgatct gggtgaactc gggcaaggtc
    14941 agactggcat caagtctgta gtcgtggcca ccccgcttcc ggctggtggt gtcctcgacc
    15001 acaccgctgg catagggctt gaaacgctcc tgcaccgtcc ggaaataacg ctcgacgatc
    15061 cccttggcat ctcctcgcct ggccggtgca ttctcgatgc gaacgccgaa ggcttgggaa
    15121 aatgcttcca ccttggtgcc attcagttcg cccttatcgg ccagcaccac atccggcaag
    15181 cctttgaccg gccagtcgct ggcatcgatc tccacgctat actggcggca atactcaacc
    15241 ttgtcggcga ccgtgttggc caatgccacc atggcactga cccaggaagg tccctcgaat
    15301 ccgacgtaca taccaacgac catgcggctg aacacgtcga tcaccatgta aacgacaggc
    15361 ctgccgacga taaggctgcg atcatgttct gaaaccagat agatatcggc gatggtggca
    15421 tcgatctggt agcggtatcc tggccccagt gcctcggtcg tcgatgtact gctgagcggt
    15481 cggaagtcct tggcaaaatc cactgcggac accctgcatg gcaaggtgtc ggtgaagtga
    15541 tattcgcgcc catagaagta acggaactgc cccaatgtag gcagttcgga ggtcggcagc
    15601 tccgtcagaa cagcacggag cagattcagc ccggcggcgt aggcatcagg aatggacgga
    15661 tgtttttcct tgagcaaacg ttcctcgatg acccggcgaa aaatgcgctc gatgtccggc
    15721 gtgacattgc tccctttccc ctccatcacc actctgggcc tgcccagctt ggcctggttc
    15781 ggctttctgc gcttgccacg ggcgcccgag ttgacatagt ccggcaagag ggcattcctg
    15841 cacatgcctc gctgccagta gcgcctcagt aacctataca ccgtctgatt ggtgacacca
    15901 tggcgctcca tgatgcctct gacaatgagc cctctggggc gccgcacgaa aagctggggg
    15961 ttgtgcacaa aatccgccag cattccccaa gcttcatcac gcttctgctg atcaagggaa
    16021 ccttcctcaa cctcccgtag gactgtttcc tcgaagggat cggcaatgct ctccagctcc
    16081 cgctccatga tcagacgctc cagctctaca atcgatatcg gttcgggcaa cgccgtgtcc
    16141 gaatcgatat cgatccagat tgcctgctcg gtaccggacc aaagcaggcg ctttcgcagt
    16201 tcccccatcc ggaacacctg attaacgggc aacactgctc agctcctgat gcatttgatg
    16261 agagttggca accaggtctt tcggcttcag ctcccggtaa ggagtgtcaa gatcgaaaag
    16321 gaaacagtgc cgggccagca actggcgtag ccaatagagc gcctgaccgg cctccagctg
    16381 gccggaggta tccagtcctt gggcaatagc ggtcagcttc cggtctgggt gacgctgaaa
    16441 ctcgagcaga aacagctgct ggtaatgggc gagatcgtcc tgagcgatgt cgccttcagc
    16501 atgggcagga tagagccatt ggatattggc aaatgccacc ttggatacct cgcgctccgt
    16561 aacgatgacc cagggaatac ctttttcctg ccagtagcgc ctttcaagct caagcctctc
    16621 gataacttca ggcttctgca ggtcggtact gtacttggcc tgaatcgcga tgctcgggcg
    16681 ctgaggatct tcgaaatcca ccagaaagtc gctggtcagt acctgcgggg tacccttgta
    16741 acaaccgtgg ggcaggccaa gctcctcggc gatgcgcacc gtatcttcga cccgcatcgg
    16801 aaactgttca cggatatcgg tgacatgggg agaccgatcc aaggtcagga agatggccag
    16861 ttcaagatcg gatagaagat ggtgtaaacg ccgggtcttg ctgccaggca ggcggtggga
    16921 gcgacccagc gaggacacgt cccttgtgta gatgaacggc ttgtagtctg atccctggcc
    16981 ttgaccacgc ccgtccttga gccttctatc gatctgggcc tgggtcagcc ccttgaatgt
    17041 tccagacatg agaaagcctg cccccgctgt gtcgatgacc tcaacatagc ggatgcaggc
    17101 tcagaatgaa actttattta tacggatgat actttattta tacggatgaa cctttatttg
    17161 catcccacaa gggtaggtga gacaacagca aaagtagaac aggcggcgat cgagacattt
    17221 attgcgagag caaaaagtcc atgtcttggc ggatatccat ggcttgagaa caatgaacaa
    17281 ccaaaaggaa ctgttgccgt atatccggag aaaattggct atgttgaata tattgacatg
    17341 gtcaagctcg gcagtctgct gagcaatgat cctcgtcatg tatatcttgt ggcgcagcca
    17401 ggcagtttca tacatccatc gatgccagtt ttgtacctga gttctggtca ggagtcatca
    17461 atctgtaccg atttacttga aacgatcatt gtctcggatg tgcgttcatt tgctcaggat
    17521 ccccgattct gtctcagcgt catggccgaa atagcctgca gggccctttc gcccgcagta
    17581 aacgatcccg gtactgcgat tgatgtcatt ggcagaggtg ttcgaatact ctccgcttac
    17641 gcgcagaata aaatagatga aactgaagtg atatatcctt cagtacatgt tgcgccactt
    17701 cagatcaaag acctactgga agactttttc ttacctgttg cacgtgatgg tgcgagtatg
    17761 agggagattc agataagagt ccttaaagga ctgtctatgc tgagtaaggg ttggcctggg
    17821 atgtttgctg aagccgcaca tatactggca tctgaaacat tagagcatgc aactcgtact
    17881 ggtcatatag cccatgacga atatcttata aaatcaattt attataattt attcagtggc
    17941 gaaaatccta ataaaaaacc atagttgcgg gacaaaggat ttgtcctctg gcaaggtgaa
    18001 agcaaagcat tccagtgaga atgagcgtct actttgagct gtgatttcaa ctgatggacg
    18061 caacacaatt attgaactca cagcgtgaag cggacgtagc taattttgca cagccctcga
    18121 ttagaaaatt taagaatcca gccttcaatg gtgtcatggc gacaggcaat accccagtcc
    18181 agtggcatat gaagccacat tgtcgctgcc gggaatttgt ttcacccatt atcgcaccgg
    18241 agaacaggca gaagacgaag cagcgcttat ggcagcgatc cttgcagatg caatgaatgc
    18301 cggagccgaa cgaatggcac agagttcacg ggatgtcacc attcaccgga tgatggtcat
    18361 ggtggagcgt catttacgcg ctgcaatcta tgcatctacc acc
//
LOCUS       DAOFQD010000095        18363 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.095, whole genome shotgun sequence.
ACCESSION   DAOFQD010000095 DAOFQD010000000
VERSION     DAOFQD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.095"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            145..1365
                     /locus_tag="QBF46_003346"
     CDS             145..1365
                     /locus_tag="QBF46_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDQ6675491.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLSSMPVKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDVPSWRRICKVLLNNDYWCRALSFSPTKSKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1338..1967
                     /locus_tag="QBF46_003347"
     CDS             1338..1967
                     /locus_tag="QBF46_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDQ6675492.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNDQLVPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1968..3128)
                     /gene="ybdL"
                     /locus_tag="QBF46_003348"
     CDS             complement(1968..3128)
                     /gene="ybdL"
                     /locus_tag="QBF46_003348"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDQ6675493.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQVIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3238..4326
                     /gene="hcxA"
                     /locus_tag="QBF46_003349"
     CDS             3238..4326
                     /gene="hcxA"
                     /locus_tag="QBF46_003349"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDQ6675494.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDNANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4336..4533)
                     /locus_tag="QBF46_003350"
     CDS             complement(4336..4533)
                     /locus_tag="QBF46_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDQ6675495.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4709..6814)
                     /gene="cstA"
                     /locus_tag="QBF46_003351"
     CDS             complement(4709..6814)
                     /gene="cstA"
                     /locus_tag="QBF46_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDQ6675496.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6995..7408)
                     /gene="entH"
                     /locus_tag="QBF46_003352"
     CDS             complement(6995..7408)
                     /gene="entH"
                     /locus_tag="QBF46_003352"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDQ6675497.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7411..8157)
                     /gene="entA"
                     /locus_tag="QBF46_003353"
     CDS             complement(7411..8157)
                     /gene="entA"
                     /locus_tag="QBF46_003353"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDQ6675498.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8157..9014)
                     /gene="entB"
                     /locus_tag="QBF46_003354"
     CDS             complement(8157..9014)
                     /gene="entB"
                     /locus_tag="QBF46_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDQ6675499.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9028..10638)
                     /gene="entE"
                     /locus_tag="QBF46_003355"
     CDS             complement(9028..10638)
                     /gene="entE"
                     /locus_tag="QBF46_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDQ6675500.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGALGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10648..11823)
                     /gene="entC"
                     /locus_tag="QBF46_003356"
     CDS             complement(10648..11823)
                     /gene="entC"
                     /locus_tag="QBF46_003356"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDQ6675501.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12012..12968
                     /gene="fepB"
                     /locus_tag="QBF46_003357"
     CDS             12012..12968
                     /gene="fepB"
                     /locus_tag="QBF46_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDQ6675502.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12972..14222)
                     /gene="entS"
                     /locus_tag="QBF46_003358"
     CDS             complement(12972..14222)
                     /gene="entS"
                     /locus_tag="QBF46_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDQ6675503.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            14333..15337
                     /gene="fepD"
                     /locus_tag="QBF46_003359"
     CDS             14333..15337
                     /gene="fepD"
                     /locus_tag="QBF46_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDQ6675504.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15334..16326
                     /gene="fepG"
                     /locus_tag="QBF46_003360"
     CDS             15334..16326
                     /gene="fepG"
                     /locus_tag="QBF46_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDQ6675505.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16323..17138
                     /gene="fepC"
                     /locus_tag="QBF46_003361"
     CDS             16323..17138
                     /gene="fepC"
                     /locus_tag="QBF46_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDQ6675506.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17135..18268)
                     /gene="fepE"
                     /locus_tag="QBF46_003362"
     CDS             complement(17135..18268)
                     /gene="fepE"
                     /locus_tag="QBF46_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDQ6675507.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKSFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4320 a         5204 c         4736 g         4103 t
ORIGIN      
        1 tataacttca gaaagctact ataactttca cctatttatt cacggcgcga atgaaaaata
       61 aaacactctg ctttacagtg tttactttat aattaacatt cttatctcac tgatcaccct
      121 gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc gctcaatatt
      181 ctggaagctg cacgagagcg tattacctgg acactcaaca ccctaccccg cgtttgtgtc
      241 tcattttctg gcggcaaaga ctctggttta atgctgcatc tgacagccga acttgcccga
      301 caaatgggca aaaaaatctg cgttttgttt atcgactggg aggcgcaatt ctcttgcacc
      361 attaactatg ttcagtccct gcgcgagttg tacaccgatg tcatcgaaga gttttactgg
      421 gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat accaacccga atggcagtgc
      481 tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag atgcgataac cgatcctgac
      541 tttttctcct tttaccagcc aggcatgacc ttcgaacaat ttgtacgtga gtttgccgaa
      601 tggttttcac aaaaacgtcc ggcggcgatg atgatcggca tccgtgcgga tgagtcctac
      661 aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt ttgccgacga taaaccctgg
      721 actacggccg caccaggcgg tcatagctgg tacatttacc ccatttacga ctggaaagtg
      781 gctgatatct ggacctggta tgcaaatcat caaagtctct gcaacccact gtataaccta
      841 atgtatcagg caggcgttcc tctgcgtcat atgcgaattt gcgaaccttt tggcccggag
      901 caacgacaag gattatggct ctatcacgtt atcgaaccgg atcgctgggc tgctatgtgc
      961 gcacgagtca gcggggtaaa aagtggcggc atttacgccg ggcatgacaa ccatttttat
     1021 ggccatcgta aaatcctcaa gccggaacat ttagactggc aggagtatgc attattgcta
     1081 ctcagcagca tgccggtgaa aacagctgaa cattaccgta ataaaatcgc catttatttg
     1141 cactggtatc agaaaaaagg catcgaggtt ccacaaaccc aacaagggga cattggtgca
     1201 aaagatgtcc catcctggcg tcgaatatgc aaagttttac tcaacaacga ttactggtgc
     1261 cgggcattat catttagccc cacaaaatcg aagaattatc agcgttataa cgaacggata
     1321 aaaggaaagc gtcaggaatg ggggatacta tgcaacaacg attaacgcag gatttaacgc
     1381 aatttctcgc cagcttgccg gaagacgatc gcatcaaagc gattaatgaa atccgcatgg
     1441 cgatccatca ggtaagtcct tttcgggaag aaccggtaga ttgtgttttg tgggtcaaaa
     1501 atgatcagct tgtgccgaat gattacaacc caaacaacgt ggcaccaccc gaaaaaaaac
     1561 tgctaaaaaa atccattgaa atagatggtt ttacccaacc cattgtggtc acgcatacgg
     1621 ataaaaatgc cctggaaatc gtggatggtt ttcaccgcca tgaaattggt aaaggctcat
     1681 catcgttaaa attacgctta aaaggttatt tgccggtgac ctgtctggaa ggtacgcgta
     1741 atcaacgcat cgccgcgact attcgtcata atcgcgctcg tggacgccat caaatcaccg
     1801 ccatgtcgga aatcgtccgt gaacttagcc agttgggatg ggacgataat aaaattggca
     1861 aagagctggg aatggacagc gacgaagtgc tgcgcctgaa gcaaattaac ggcctgcaag
     1921 agttgtttgc cgaccgtcaa tattcccgcg cctggacggt taaataacta aagctggcgc
     1981 aggcgttcag ctgctgccag caacgtcgat tccttcttgg caaaacagag acgaatcagt
     2041 ttatggggga agggatcggc gcaaaatacc gacagcggga tcgccgctac gccgtgctcc
     2101 tgcgtcagcc actggcaaaa ctcaacatca tccagggtag ataccgcgct gtaatccacc
     2161 agcaaaaagt atgtaccttc acacggtaaa atctccagcc ggctttcatt taaggcattt
     2221 accagaatat cgcgcttctg gcgataaaag tccggtaacg caagataatg ctcaggttct
     2281 gcacgtagca tatcggcaag tgccagctgt gccggggtat ttacagaaaa ggtcagatac
     2341 tgatgtacct tgcgaatttc ggcactgatg ggcgctggcg caacgcaata acccactttc
     2401 cagccggtca tatgataggt cttgccaaac gatgacaccg ccaccgcccg ctcacgcaac
     2461 tgcggatgcg ccagcacact ggcatggccc tgctgtgaaa agttgatgtg ctcgtagact
     2521 tcatcgctaa tgacaaaaat ctcgtgcccg gcaattacct gccacaaagc ggcgaaatca
     2581 gcctgctgcc agacagtagc actggggtta tgcggggtgt tgaggatcac cagtcgggtg
     2641 cgctcgctta acaatgcggc aaattcctgc cagtcaacgc gaaaatgcgg tggttgcagt
     2701 gcaatacgtt taactattcc tcccgaaagc gcgatggcag gggcgtaact gtcatagctg
     2761 ggatcaaaac aaatcacttc atcgccattg cgcaccaatg cggtaatcgc cgcgtataac
     2821 gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catctggttg atagccatac
     2881 aaacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt catgggcgcg
     2941 tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata gcgcggacca
     3001 tcaaaatcag gaaagccttg cgacagatta atcgcctggt gttgctgcgc cagcgcgctc
     3061 atctgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat cagagggtta
     3121 tttgtcattt tttattgtgc ctgacgaagt tgcgtgttga aggcactata acacgatgct
     3181 agtatttggc aatcaagacg tttagatgtc taaataaaac aataaggaca acacaacatg
     3241 cctcacaatc ctatccgcgt ggtcgtcggc ccggctaact acttttcaca tccaggaagt
     3301 ttcaatcatc tgcacgattt tttcactgat gaacagcttt ctcgcgcggt gtggatctac
     3361 ggcgaacgcg ccattgctgc ggcgcaaacc aaacttccgc cagcgtttga actgccgggg
     3421 gcaaagcata ttttgtttcg cggtcattgc agcgaaagcg atgtacagca actggcggct
     3481 gaatccggtg acgatcgcag cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc
     3541 gcgaaagccc tcgcccgccg tctcggtctg ccatttgttg ccgctccgac gatcgccgcc
     3601 acctgcgccg cctggacacc gctctccgtc tggtataacg atgccggaca ggcgctgcat
     3661 tatgagattt tcgacaacgc caattttatg gtgctggtgg aaccagagat tatcctcaat
     3721 gcgccgcaac aatatctgct ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg
     3781 gtggtgctgg ctccgcaacc agaaacgttg ccgctaaccg tgcggctggg gatcaataac
     3841 gcgcaggcca ttcgcgacgt cttgttaaac agtagcgaac aggcgctgag cgatcagcaa
     3901 aatcaacagt taacgcaatc attttgcgat gtggtggatg ctattattgc tggtggtggg
     3961 atggtcggtg gtctgggcga tcgttttacg cgtgtggcgg cggcccatgc cgtgcataac
     4021 ggtctgactg tcctgccgca aaccgagaag tttctccacg gcaccaaagt cgcctacgga
     4081 attctggtgc aaagcgcctt gctgggtcag gatgatgtgc tcgcgcaatt aactggagcg
     4141 tatcagcgtt ttcatctgcc gactacgctg gcggagctgg aagtggatat caataatcat
     4201 gcggagatcg acaaagtcat tacccacacc ctgcgtccgg tggaatccat tcattacctg
     4261 ccggtcacgc tgacaccaga tacgttacgt gcagcgttcg aaaaagtgga atcgtttaaa
     4321 gcctgacgta catatttagc agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg
     4381 ccgctcacgg aaaaactcct cgtaggtcat cggcgtttga tcgggatggt taacccgcat
     4441 atgttcgaca tagttgtcgt aatcaggcat accaatcatc agcttcgccg cctgacctaa
     4501 atattttccg gcttttgcca gtgaatcaaa catcttggcc tccgctaacc atgttgctga
     4561 aagagtgccg gatgcggcgt gaacgcctta tccggcctac aaatgctatc aacgcgctcg
     4621 gtcagtcccc tcgccccatc ggggagaggg tgagggggaa ccctcggcgc catgccaaca
     4681 ttcgccaaca ttctccctca ctctgacttt agtgtgcgcc ttttgcctgc gccacgatct
     4741 cctcgacatt ttccggcatt ggctcatacg gcgtttcttt cgccgttggc ttcggctctt
     4801 tcaatgccgc aagtgccgtc ttaatcgaga acagtgccag aaccaccacg accaccataa
     4861 agaagatggt taacccggca tccagacggt tgttgaacac cagttgcgcc agctgtgact
     4921 cagtatattg cgacggaatg ttgccgctgt cgatcattgc ctggaactta ttagcaatgg
     4981 ccaggaagcc gactttcgca tccgggctaa acgctttctg ccagccagcg gtcagggtac
     5041 aaatcagcag ccaggccgtt ggtaccagcg ccacccaggc gtaacgttga cgtttcattt
     5101 tgaacagcac cacggcacag agcatcagcg ccatccctgc cagcatctgg ttggcaatac
     5161 caaacagcgg ccacagagtg ttaataccgc ccagcggatc gaccacaccc tgatggagga
     5221 agtagcccca cgccagtacg cacagcgccg ttgccagcag gttagcaggc agtgaatcgg
     5281 tccgcttcag gccaggagac accacgccca gcagatcctg caacataaag cgcgcagcac
     5341 gcgtacctgc atccaccgcc gtcagaataa acagtgcttc aaacaaaatg gcgaagtgat
     5401 accagaacgc cacatccatc atgccgccca gcgcgccgtg cagaatgtag gccatcccca
     5461 ccgccagcgt cggcgcaccg cccgcacggg aaatgatcga ctgttcaccc acttcgctgg
     5521 caatctggtt tagcgtatct ggtgtaatgc taaagcccca gctactcacc acctgcgcgg
     5581 cagaagcgac cacatccgcc gtccctgccg gagccagcac cgccatcggg ctgttcatgg
     5641 caaaatacac gcccggatcg atgatacagg cggaaaccag cgccataatc gccacgaagg
     5701 attccattaa catcccaccg tagccgataa agcacgcctg cccttcgttc gccagcatct
     5761 tcggcgtggt cccagaagag atcagcgcat ggaagccaga caccgcgcca caggcgatgg
     5821 tgataaacag gaacgggaac aggttaccgg tccataccgg gccagtgcca tcgacaaatt
     5881 tggtcagcgc aggcatggtc agcgtcgggc gcataatcaa aatgcctacc gccagaccga
     5941 cgatagtccc gattttcagg aaggtagaga ggtagtcacg cggggccagc agtaaccaca
     6001 ccggcagcac cgccgccaca aaaccgtaac ccaccagcat ccaggtcaac tgcacgccgg
     6061 taaagtcaaa gtacggtgcc caggtcggac tttctgccac ccagccgcca gagataatgg
     6121 cgaaaatcag gaataccaga ccgatgaccg acacttcgcc aatacgcccc ggacgcagat
     6181 agcgcaggta gatccccata aacagcgcca gcggaatggt gaacgcaacg gtatatgttc
     6241 cccacgggct atgagtcagg gctttcacca cgatcatcgc cagcactgca aggataatga
     6301 ccatgatcat aaagcaggcc accagcgcaa tcaccccggc ggttggcccc atctcttctt
     6361 tgaccagctc acccagcgag cgaccgtcac ggcgcgtaga aacaaacagc accatgaaat
     6421 cctgcaccgc accggcgaga accaccccag cgagcagcca gatcatcccc ggcaggtagc
     6481 ccatttgcgc cgccagtacc ggccccacca gcggacctgc tccggcaatg gccgcaaaat
     6541 ggtgaccgaa cagcactttc ttgtccgtcg gcacatagtc cagcccgtcg ttatggcgca
     6601 ccgctggcgt catacgcgtc gggtcaaccg ccagcacatt tttggcgata tacagcccat
     6661 aaaaacggta agcgatcaga tagatacaga ccgacgccac cacaatccac agcgcgttga
     6721 tctgttcccc acgatttaaa gcaatgtatc ccagagcaaa tgctcccatt acagagagca
     6781 ctgtccagac gaggtatttc cctgatttgt tcatagttgt tatccgtgtg cgtgtccata
     6841 gagatgttac attttgtttc tataacatct ttgtacaatt taacaacaca gcaaccatgt
     6901 aaatcggagt tgaaaccaat atttaactta acaatgttaa ctcgatcact ccgttaaccg
     6961 agttttcatg caatctcatc taattctgtc gggttcatcc caaaactgcc gtacccagcc
     7021 gacaagtgca gcaacgccgc ccctgttcat cgaaaacgac gatttcccag ctctgatttt
     7081 gccgaccaag atgcagcggc tggcagacgc cgcgtacctt tccctcagac accgggcgat
     7141 ggtgtgttgc attaagttct gtgcctacca cacactgtcc gtcgcgggtc atcataaatc
     7201 cggccatcga tcccagcgtt tccgccagcg ccgccgacgc gccgccatgt agtaaaccga
     7261 acggctgatg agtacgggta tcaaccggca tttcggcttc cagcacatca tcgcccagac
     7321 gggtatacac aattcccaga tgcgccacca ttgtgttatc gctggtggcg ttcagttcgt
     7381 cgagcgttaa atggcgtttc cagatcatgc ttatgccccc agcgttgagc cgccatcgac
     7441 cacaatatcc tgtagggtga tatggctggc gaggtcagag gcgaggaaca aaatcgtgtt
     7501 ggcgatctct tgtggacggg cgattttccc cagcggaatg ccgagtttaa actgctcgcc
     7561 aaagccgcga atacgctgtt cttcggcgtc atcgctcacc cacagcgtgc gttgcatatc
     7621 ggtgtcggtg gagccaggcg aaaccacatt acagcgcacg ccgctacccg ccagttccag
     7681 cccgacgctc aacgccaggc ttttcagcgc cgctttcgat gcgccataag cactcatgcc
     7741 aatacgcggc gtgtgcgcgg cgtcggacgc cacagtgaca atcgccccgc cccgctgacg
     7801 gcgaaactgg ttcatggttt gctggaacag gttaaacgca ccgccgacgt taaccgcaaa
     7861 agtctgctgc cagtcctctt tactgagctg atcggtcgcg cccatgcgca aaattcccgc
     7921 cgcattgacc agcacgtcca gtcgctccgt ttcagctaac agtcgctgac atacctgcgc
     7981 gacctgcgca gcatcggcaa catccatcac ttcggtcgca aagggatatt gctcctgagc
     8041 gaacgcttga tcaaaaccgg taactttcgc tcccgcttca acaaacgcca gcgccgtggc
     8101 gtagccgata cctttacctg cgccggttac ccagacattt ttaccgctga aatccattat
     8161 ttcacctcgc gggagagtag cttccaccag gcgtcgatgg tcgggttttt cgccagcatg
     8221 acaaagtcga tatcgccatg cactttgcgc cagcgcgccg ccagcgccat catgcgcacc
     8281 gaatccagac cgtagtcgat caggttgtcg tcatcgaacg gttcatcgga ctcgtccagc
     8341 aacggcagga taacctcacg cagcgccgct ttgctggcgg ggacaggtgc tggcagtaat
     8401 tcttcagtca tcaccacccg gccagaccgc ccggccacat atttcagcga catcaaatgc
     8461 tcgtcacggc tgaaatcggc cagcgcatcc gccaccataa acggtttaat atcgcgcata
     8521 aatgcgtcgg ttgcggtggt catacagcca atgtgggcat ataccccggt aataatcagc
     8581 tggttacgtc cactctcttt gagcatttgc tccagcggag aacgatgaaa cgcgctgtag
     8641 cgccacttca ccagcaccgt gtcgtcggca tctggcgtca ggcgatccac caccttttgc
     8701 tgttccggcg agcgggtcag gcccggcccc cacatatcat tcaacagcgc ccgatcttca
     8761 tcgctctgct ctttcggctg ggcggtgtaa taaaccggga tattgtgctg tttgcagtag
     8821 tcgcgcagcg cagcaatatt cgcgatcacc tgctccatca tcgggcagtt ctcgccccag
     8881 aagctgacaa aatagtcctg catatcatgg attaacaacg cggcacgttg cggttcaaag
     8941 gcccagtcaa ctttattctg cggaatatcg tgagactccg gcagtgcgta agcctgtaat
     9001 tttggaatag ccatcgtgtt ctctccttca ggctgatgcg cgtgacgcca gccactgacg
     9061 taattgtttt ttatcgactt tcccgaccgc cgtcagcgga agtgaatcca cacactccac
     9121 gcgatccggt aatttaaatt cggcaatacc ctgttcacgc aggaaacgac gcacctgcac
     9181 cgcgcgcagc ggctctttta ccaccagata agcacagctt ttttcgccca tcagctcatc
     9241 ttccatgctc accagtgcgg catagatcac cgccggatgg cgcagcagca ggttttcgat
     9301 ctcttcggca gcgatcttct cgccgccacg gttgatctga tctttctcgc gcccctgcac
     9361 ggtgatgtaa ccctctggat caatagagat cagatcgccg gaacagtaaa aaccgttggc
     9421 atcaaaggca ctggcattgt gctgcggact tttgtaataa ccgcggaagg tgtacggccc
     9481 gcgcgtcatc aggcgtccaa cttccccttg cggcagtgga tttccttcag catcagcaac
     9541 ccatacttca tcgtccggac acattgggta accctgggta tggataattt tctccgcgct
     9601 atcatcaagt cgggtgtagt tcaccagccc ttccgccatg ccaaacacct gctgcaacag
     9661 gcagccaatc tcagcgggaa tacgcgccgc aagggtggct gaaagacgtg cgccgccgac
     9721 ctgtaacagt ttcagcgagg caagctgcgc ccggctttca ccttcggtca gcgcctgcaa
     9781 ccacaggctg actgccggtg gcaccagcgc ggtgacgttc acctgatgtt tttcaatcaa
     9841 tgggaagcaa agcgtggcgc tgggatcggc cgccagaaca accgttcctc cggcaagaaa
     9901 gacgcccagc gctcccggcg aactcatggc gtagttatga gccgccggga tcgcgcacag
     9961 gtagcgtgtc tgttgtgtga actgacaaat ctcgacgcta cgacgcacgc tgtagtagta
    10021 gtcgttatga gtgcgcggga tcagtttcgg tgtgccggtg gtgccgccgg aaagctggaa
    10081 ataggccact tcatcagcag gtgatggcgt ggcagtaaaa ccctctgccg gatggttaat
    10141 cgcatcctgc aagttatgct caccgctgtc gttgtgcagt tgcaccacgc gaatggaaga
    10201 atgctctgca acaaatgtgt tgaggaaatc atccccgcta aacagcgcat gttggcgatc
    10261 ggcaatcagc aatgcgggtt caatctggct ggcataggcg ttcagttcac tacgctgatg
    10321 gctgaacaac gccagcaccg gcgcaacgcc cagtttcagc agtgcgaaaa aggtaatgta
    10381 aagttcagcg acgttaccca gttgtaccag cgcggtttca ccaggtttaa tgccctgacg
    10441 acgtaaacta cacgcgaggt tatccgccgc ctgattcagc tcccggtaac tcaactgtcg
    10501 ctcgccgtcg ataaccgcga tgctgtcact cgcagcgtgg cgagtcagaa tgtcggtcag
    10561 cggcaaatcc tgccagtagc ctttttcccg atagcgacgg gcaaactctt ccggccagcg
    10621 ggtgaatgga atgctcatcc tcgctcctta atgcaatcca aaaacgttca acatggtaga
    10681 aagtttgacg cctgtttcgc gccactcacc caacggtgac gacgcaggca caatccccgc
    10741 tccggcaaac agacgcacct gattttcccg cagcttcgcg cagcggatgg tcaccaccca
    10801 ttcgccgtta ccttcgctgt cacaccaacc cacaatgccg ccaaacagtt cgcggtcgaa
    10861 tggctccagt tcagcaataa cctgggtcgc ggcctgatgc gggaaaccgc tcagcgcggg
    10921 ggttggatgc agcagacagg ccagagtcag tgcgttttct tgcgaattcg ctttaccttc
    10981 aaagggagtt gcgagatgcc acagcgtcgg cgtggtaatc aattgtggag aggagggaac
    11041 gtgtaactca ctactgcgtt cgcgcagtac ctctttcatc gcctgagtca ccagttcatg
    11101 ttcatggcga tctttttctg acgccagcag acgattaccc gcttcgcgat cgagcacttc
    11161 atccggctga cgacgcgcgg aaccggctaa cggaatggag ctaaaacgct caccgtcttt
    11221 acgtagcagc agttccgggc tggcccccag cagaacgcca ccatctgcca gcggaacatg
    11281 gaagttgtaa ctaaccgggt tttgcgcaat caaccgttcc agcaatacgc cactatcaat
    11341 ggcggcgtca gtggtgatat caatcaaccg tgacaacacc actttgtcga cctgcggcgt
    11401 ggcggtaagt gcggcggcgc gggcaaccat ctgttcaaac gtggtttgct ccggaattgc
    11461 ctggcgttcc accacattca gcgactggct gcgggtgaaa cggcgtgcgg aagcttgttt
    11521 ttcctgacgg gagaacgact gccaggattc aggaatatac agcgacgaag gctgacgtgg
    11581 atcgaaggga atcgccccga ccatcaccgg atttttgatg ccctgcgctt tggcatcggc
    11641 aaacagcgcg gcgagttttt gctggaaggg actgtcgggc gaatccccgt tcacagccgg
    11701 ttcatcgaag cgggcgaaac atcctgacgt cgtaaaactg cggtacggcg acataaagaa
    11761 aaagcgattg ggcgcaagtg ttgccatggt ctgctgtact tcctcagcca gtgacgtatc
    11821 catatcatcc tccacaaaat gataaaggct ttaataatga ttatcattta tattttcggt
    11881 cgctaaccta aaagcaaacg cgcactatgt caactcttga ggtaacgcaa tctgcaactc
    11941 ggggttgcat ctggttgtgg ttacaatgaa aatgagaagc attaacttca ttaataacag
    12001 gaagctgatt tgtgagactc gccccgctct accgcaatgc ccttctatta acaggacttt
    12061 tgctttcagg aatagcagca gttcaggccg ccgactggcc gcgtcagatt actgacagcc
    12121 gtggcactca taccctggaa agccagccgc agcgtattgt ttccaccagc gtcaccctga
    12181 ccggctcact gctggcgatt gatgctccgg tgatcgccag cggcgcgacc acgccgaata
    12241 accgcgtcgc ggatgaccag ggctttttac gccagtggag caaggtggcg aaagaacgca
    12301 aactgcaacg gctctatatc ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg
    12361 atctgatttt aattagcgca actggcgggg attcggcgct ggcgctgtat gatcagcttt
    12421 ccaccatcgc tccgacatta atcatcaatt acgacgacaa aagctggcag tcgctgttaa
    12481 cgcaacttgg cgaaattacc gggcatgaga aacaagcggc agagcggatt gcacagtttg
    12541 ataagcaact ggcggcggcg aaagagcaaa tcaaattacc gccgcagccg gtcactgcca
    12601 ttgtctatac cgccgctgca cacagtgcca atctctggac gccggaatca gcacaagggc
    12661 agatgctgga acaactcggc tttacgctgg cgaagttgcc cgcaggctta aacgccagcc
    12721 aaagccaggg caaacgccat gacatcattc agcttggtgg ggaaaatctg gctgctgggt
    12781 taaacggtga atcgctattc ctgttcgctg gtgatcagaa agatgccgat gctatttatg
    12841 ctaatccgct gctcgcgcac ctgcctgcag tacaaaacaa gcaggtttat gcgctgggaa
    12901 ccgagacgtt ccgtctggat tactacagcg ccatgcaagt gctggaacga cttaaggcgc
    12961 tgttttaagc attaaccgtc agacgctgtc acctgcggcg gcgtctggcg aaaacgtcgc
    13021 aactccacca gcaccagcaa taacaacacg ccgataatca acaaaccaaa accgctcgcg
    13081 cttgcggaag caaccggtgt catcatcgcg cccaaaccgc ccagcagcgc cgcgcctatg
    13141 gcatcgcccg tcacgttttg tgccgtccac aaaccgttaa tccgccctaa catcgcttcc
    13201 ggggtttgcg tttgcagcat tgtgtattgc agcaacgagc tgaccgcact caaccagccg
    13261 aacagcgcca gacaaaccac gcctaaaatc cacatcggca tcaggccaaa cagaccaatg
    13321 gcgaggaacg atcccagcgt ggagagcagc atcaataacc ctggtcgcgc actatgtgcc
    13381 agcttcccgc tggttaacgc accaatagcc gcgccgagcg ggatcgccgc gtagagaaaa
    13441 ccaatctgtg ccgccgacat ctgccagttg tcagccagcg ccggatacag tacccgcacc
    13501 gcgctcgcca tcgtcaataa accacccagc agcgcaatcc ctcctaccag cgggctggcg
    13561 agcagaaaac gaaatcctgc cagtaatgat ttcaacggat gctcacgcgg ctgcggtggc
    13621 ggtggcagtg ccggaaggct taacaacggt agcaaggtaa taaacgtacc cgccgccgcc
    13681 agcccgtagt tccaggccac gccgccagtc gccagcagta aaccgccaat catgggcgaa
    13741 atcaccgacc ccagacgcac ggtcaacatg gtgatcgccc cggcctgcat taagttttca
    13801 cgccctacca gtgctggtgt cgccgccaat agcgccgtaa cgccaagcga tgcgaaaaaa
    13861 ccatcccata aaccaagtaa atagattgcc agcaatgacg gctccggcag cagtgcatta
    13921 aggcacagtc caatgaagcc aatgccacag gtgccgcgcg ccagcaaaat cacttttttg
    13981 cgctcatagc gatccgccag cacaccgcca accatcaggc caacaaacat cgcgccgccg
    14041 gtcagcgtca ccgaaagccc cacctgccag gtagaatgcg tcatcatctg gatctgcacc
    14101 ggcaccgcga cgccgagcaa acccagagac acaattgaga taaaacgagc gaggaatact
    14161 gcgcgaaacg ccgggtgcgt tttcaacagg ctgaggttaa gcagccagga ttgtttattc
    14221 attacaatgc cttgccatca aatattttaa atacctattc ttaggctgca catgctaaca
    14281 tatccaaata agatcgataa cgataattaa tttcattatc atggaagttc gtatgtctgg
    14341 ttctgttgcc gtgacacgcg ccattgccgt gcccggattg ctgttattac tgattatcgc
    14401 gacggcatta agcctgctca ttggggcaaa atcactcccc gcttccgtag tgctggaggc
    14461 cctctccggc acctgccaga gcgccgactg caccatcgtg ctcgacgcgc gactgccgcg
    14521 cacccttgcc ggtttacttg caggcggcgc gcttggcctt gccggggcgt tgatgcaaac
    14581 cctcacccga aatccacttg ccgaccccgg cttgcttggc gtgaatgccg gagccagctt
    14641 tgccattgtg ctgggtgcgg cgctgtttgg ttactcatcc gcgcaggaac aactggcgat
    14701 ggccttcgcc ggggcgctgg tggcctcgtt gattgttgcc tttaccggca gccagggcgg
    14761 cgggcagtta agtccggtgc gtttaaccct ggcgggcgtg gcgctggcgg cggtgctgga
    14821 agggctgacc agcggcatcg ccctgcttaa tcctgacgtc tacgatcagt tgcgtttctg
    14881 gcaagccggt tcgctggata ttcgcaatct acatacctta aaagtggtgc tgatcccggt
    14941 gctgatcgcc ggagcaactg cgctattact gagtcgcgcg ctgaacagtt tgagcctcgg
    15001 cagtgacacc gcgacggcgc tgggcagtcg cgtggcgcgc acacagttga ttggtctgct
    15061 ggcgattacc gtgctttgtg gtagtgcgac ggcggtagtt ggcccgattg cctttatcgg
    15121 cctgatgatg ccgcacatgg cgcgctggct ggtaggtgcc gatcatcgct ggtcgctgcc
    15181 cgtcacgcta cttgctaccc ctgccctgct gctgtttgcc gatatcatcg ggcgggtgat
    15241 tgtgcccggc gaactgcgcg tttctgtggt cagtgcgttt attggtgcac cggtgctgat
    15301 cttcctcgtg cgacgtaaaa cgcgaggtgg tgcatgattt acgtctctcg ccgattactc
    15361 atcacctgtt tgctgctggt ttccgcctgt gtggttgcag gtatctgggg attacgcagc
    15421 ggtgccgtca cgctggaaac ctcgcaggta ttcgccgcgc tgatgggcga tgcgccgcgc
    15481 agtatgacga tggtggtcac cgaatggcgt ttaccacgcg tgctgatggc gctgttgatt
    15541 ggcgcagcac tgggcgtcag tggcgcgatt tttcagtcgc tgatgcgtaa cccgctcggc
    15601 agccctgacg taatgggctt taacaccggg gcgtggagcg gcgtgctggt ggcgatggtg
    15661 ctgtttggtc aggacctgac ggctatcgcg ctgtcagcaa tggtgggcgg cattgtcact
    15721 tcgctgctgg tctggctgct cgcctggcgc aacggcatcg acacctttcg gttgattatt
    15781 atcggtatcg gcgttcgcgc catgctggtg gcctttaata cctggctgtt gctgaaagcg
    15841 tctttagaaa cggcgctaac agcaggtttg tggaatgccg gatcgctcaa cggcctgacg
    15901 tgggcaaaaa cctcgccttc cgcacccatc attatattga tgctcattgc cgccgcctta
    15961 ctggtacgcc ggatgcgctt gctggaaatg ggcgatgata ccgcgtgtgc gctgggcgtc
    16021 agcgtcgaac gttcgcgtct gttaatgatg ctggttgcag tggtgcttac cgctgcggca
    16081 acagcgcttg ccgggccgat ttcctttatt gctttagtcg caccgcacat tgcccgacgc
    16141 attagcggca ccgctcgctg ggggctaacc caggcggcgc tatgcggggc gctgttactg
    16201 ctggcggccg atctctgcgc ccaacaactg tttatgccgt atcaacttcc ggttggcgtc
    16261 gttaccgtca gcctcggcgg tatttacctt atcgtcttgt taattcagga gtctcgcaaa
    16321 aaatgaccga atcagtagcc cgtttgcgcg gcgaacagtt aaccctggga tatggcaaat
    16381 ataccgttgc ggaaaatctg actgtagaaa tacctgatgg tcacttcacg gcaattatcg
    16441 ggccaaatgg ctgcggtaaa tccacgttac tgcgtacctt aagccgcctg atgacgcctg
    16501 ctcatgggca tgtctggctg gatggcgagc acattcaaca ttacgccagt aaagaggttg
    16561 cacgccggat tggtctgttg gcgcaaaatg ctaccacgcc gggcgatatc accgtgcagg
    16621 agctggtggc gcgtggacgt tatccgcatc aaccgctgtt tacccgctgg cgcaaagagg
    16681 atgaagaagc ggtaacgaaa gcaatgcagg ccacgggaat aactcatctg gcagatcaaa
    16741 gcgtggatac cctttctggc ggacaacgcc agcgagcgtg gatcgcgatg gtgctggccc
    16801 aggaaacggc aattatgctg ctcgacgaac ccacgacctg gctggatatc agtcatcaga
    16861 ttgatttgct ggagttgtta agcgaactga accgcgaaaa aggctatacc ctggcggcgg
    16921 tgctgcacga tcttaatcag gcctgtcgtt acgccagcca tttgattgca ttgcgggaag
    16981 ggaaaattgt tgctcaggga gcgccgaagg agattgtcac tgctgaactg attgagcgca
    17041 tttatggtct gcgctgcatg atcattgacg atccagttgc cggaacgccg cttgtggtgc
    17101 cgctcggacg aacggcacct tcaaccgcaa atagttaaac taagtggtct gccatcatgg
    17161 catcctgttt tctggatgcc atcgcatagc gcaacagcac gccaccacaa gccaccatcc
    17221 cgccgatcaa cgcggaaagg atcacaataa tcgccttacc cggaccgtct tttttcactg
    17281 gcaatgacgg gcttaactga tatttaaacg gcgtaaaatt cacatcgttg acatgtgctt
    17341 ttgttaattg ctcgacaaga tactgccgat tacgtaattc accgttcagt tccgcaacgt
    17401 cagtgaccgc tttttctatt tccagtttgc gttcaatacc gtctgcaccg agagaaatag
    17461 aaaaatcggg gtcatcttta acggcctgac cattactgta aacgggcttt ttaattcctg
    17521 ccgcgttggc aatgtcgagt gaataattga ggcgctgaat gtttgcatca agttgatttt
    17581 tcgttttaat gcgatcctga gccagttttt ctttttcaaa ctgggttttg atctccagtt
    17641 tattacggac gttttctagc gactctttca ccaccaacgt agagatataa tcgatatacc
    17701 cgctcaaaac ggtctgcgcc tcttcactgg ttggcgcggt aaaacttagc gtccaggagg
    17761 tatacagtga cggttcatct tttttcttac tggcattgtc atcaaccgct ttcatttttt
    17821 cgctcaatgc gacaattgcg cgatgcaaat ccagttcgtc gattttcgcc tcttttaatt
    17881 ggtccatcac ataaggtgat gaacgcaggt actcttccag caagctaacc gactgaaact
    17941 tcttgataaa caggttaaat gcttctgtac gatcaatttt gatatccaga tccagcacac
    18001 gaagcttagt gaatgatttc tccaactctt gccactgaac aggttctgga ggcgtgacaa
    18061 ccgccgcgct agtccatttt tgcggcagga tgaaagagat cagcaagcct gcgcaggcaa
    18121 acgcaaaaac gaccgccatg accgtttttt tggcccgcca taaaactgag attagattaa
    18181 gtaaatcaat ttcattatta ctgggcgacg ccagaggata atcgggaaaa tgagcgtcac
    18241 ttccctgttt aatattcagt gatgacatgg ggtataacct gaaaatgagt ccaatagggg
    18301 gaaatctcca cggcgctcac tttaccagtt ggtgataatt atccgaagct gaagtttgta
    18361 aat
//
LOCUS       DAOFQD010000096        18325 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.096, whole genome shotgun sequence.
ACCESSION   DAOFQD010000096 DAOFQD010000000
VERSION     DAOFQD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.096"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            21..800
                     /locus_tag="QBF46_003363"
     CDS             21..800
                     /locus_tag="QBF46_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDQ6675508.1"
                     /translation="MEHKPTIRVLEILEALAAENQGVSLTHLSEKTGILKGTIFPILK
                     TLVEQRYISYDANTQLYNLGISCSILSRAFLDRSYWLKMVYKEMNNIVKECNEVCQMG
                     ILDGADVLYINKVQAAQTVQLVSHIGTRLPAIYSALGKAIICEYSDQQIRQLYPDGFV
                     PMTSYSVTTLEQLRQQLEDARVNGYAFDNREINEETVCYAVALKQRNKTLAAISISIP
                     VFRATKEKIADVTRILLNARSRIEQELNTLQDINIGIDSHH"
     gene            complement(877..1608)
                     /gene="rlmB"
                     /locus_tag="QBF46_003364"
     CDS             complement(877..1608)
                     /gene="rlmB"
                     /locus_tag="QBF46_003364"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDQ6675509.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1788..4229)
                     /gene="rnr"
                     /locus_tag="QBF46_003365"
     CDS             complement(1788..4229)
                     /gene="rnr"
                     /locus_tag="QBF46_003365"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDQ6675510.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFCGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(4268..4693)
                     /gene="nsrR"
                     /locus_tag="QBF46_003366"
     CDS             complement(4268..4693)
                     /gene="nsrR"
                     /locus_tag="QBF46_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDQ6675511.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLMELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4898..6196)
                     /gene="purA"
                     /locus_tag="QBF46_003367"
     CDS             complement(4898..6196)
                     /gene="purA"
                     /locus_tag="QBF46_003367"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDQ6675512.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(6300..6497)
                     /locus_tag="QBF46_003368"
     CDS             complement(6300..6497)
                     /locus_tag="QBF46_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDQ6675513.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6579..7583)
                     /gene="hflC"
                     /locus_tag="QBF46_003369"
     CDS             complement(6579..7583)
                     /gene="hflC"
                     /locus_tag="QBF46_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDQ6675514.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7586..8845)
                     /gene="hflK"
                     /locus_tag="QBF46_003370"
     CDS             complement(7586..8845)
                     /gene="hflK"
                     /locus_tag="QBF46_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDQ6675515.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8931..10211)
                     /gene="hflX"
                     /locus_tag="QBF46_003371"
     CDS             complement(8931..10211)
                     /gene="hflX"
                     /locus_tag="QBF46_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDQ6675516.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(10288..10596)
                     /gene="hfq"
                     /locus_tag="QBF46_003372"
     CDS             complement(10288..10596)
                     /gene="hfq"
                     /locus_tag="QBF46_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDQ6675517.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10682..11632)
                     /gene="miaA"
                     /locus_tag="QBF46_003373"
     CDS             complement(10682..11632)
                     /gene="miaA"
                     /locus_tag="QBF46_003373"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDQ6675518.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11625..13472)
                     /gene="mutL"
                     /locus_tag="QBF46_003374"
     CDS             complement(11625..13472)
                     /gene="mutL"
                     /locus_tag="QBF46_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDQ6675519.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(13482..14819)
                     /gene="amiB"
                     /locus_tag="QBF46_003375"
     CDS             complement(13482..14819)
                     /gene="amiB"
                     /locus_tag="QBF46_003375"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDQ6675520.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14838..15299)
                     /gene="tsaE"
                     /locus_tag="QBF46_003376"
     CDS             complement(14838..15299)
                     /gene="tsaE"
                     /locus_tag="QBF46_003376"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDQ6675521.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(15271..16818)
                     /gene="nnr"
                     /locus_tag="QBF46_003377"
     CDS             complement(15271..16818)
                     /gene="nnr"
                     /locus_tag="QBF46_003377"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDQ6675522.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEATLAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16817..17956
                     /gene="queG"
                     /locus_tag="QBF46_003378"
     CDS             16817..17956
                     /gene="queG"
                     /locus_tag="QBF46_003378"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDQ6675523.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPIEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17939..17992)
                     /locus_tag="QBF46_003379"
     CDS             complement(17939..17992)
                     /locus_tag="QBF46_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675524.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(18226..18301)
                     /locus_tag="QBF46_003380"
     tRNA            complement(18226..18301)
                     /locus_tag="QBF46_003380"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18266..18268),aa:Gly,seq:gcc)
BASE COUNT         4213 a         5177 c         4577 g         4358 t
ORIGIN      
        1 tggtaaggga aatagtcatt atggaacata agcctaccat ccgggtgtta gagatactgg
       61 aagcactggc agcggagaat cagggtgttt cattaactca cctttcggaa aaaacaggca
      121 tcctgaaagg aacgattttt cccatcctca aaacgctggt tgaacagcgg tacatcagtt
      181 atgacgctaa tacccagctt tataatctgg ggatctcctg ctcgattctg tcccgtgctt
      241 tcctcgacag atcgtactgg ttaaaaatgg tttacaaaga gatgaacaac attgtcaaag
      301 agtgcaatga agtttgtcag atggggatcc tggacggtgc agatgtttta tatatcaata
      361 aggtgcaggc ggcacaaacc gtacaactgg tttcacatat tgggactcgt ctgcctgcta
      421 tctattctgc gctgggaaaa gccattatct gtgaatacag tgatcaacaa attcggcaac
      481 tctaccctga cggttttgtt cccatgacat cgtatagcgt tacgaccctg gaacaattgc
      541 gccaacaact tgaggatgcc agagtaaatg gctatgcatt tgataacagg gaaatcaatg
      601 aggagacggt atgttatgct gttgccttaa aacagcgaaa taaaacgctt gccgccatca
      661 gcatttcaat acccgtattt cgtgcgacaa aagaaaaaat tgccgatgtc acgcgtatcc
      721 tgctgaatgc acgttcgcgt attgaacagg aactcaacac cttacaggat atcaatatcg
      781 gtattgattc ccaccattaa gggtattttc cattcgaagc ccgatacgta aaaattatcg
      841 ggctaacaaa atatccattg aatggatagc gatgaactag ctgcgctggc gcaccgcttc
      901 aaataagcaa attccggtcg caaccgaaac gttcagggaa gaaacgcttc cagccatcgg
      961 gatgctgatc aactcatcgc aatgttcacg agtcaggcga cgcatacctt caccttccgc
     1021 acccatcacc agcgccaggc gtccggtcat tttgctctga tagagtgtat gatccgcctc
     1081 gcctgccgta ccgacgatcc agatattctc ttcctgcaac atacgcatgg tgcgcgcaag
     1141 gttagtcacc cgaatcagcg gaacgctttc tgccgcgccg caggctactt ttttcgccgt
     1201 agcgttgagc tgtgcggagc gatctttcgg cacaatcacc gcatgaacgc ctgcggcgtc
     1261 cgcactacgc aggcacgcgc cgaggttgtg cggatcggtt acgccgtcga ggatcagcag
     1321 gaacggttga tcgagcgaag cgatcagatc cggcagatcg ttttcctgat actgacgtcc
     1381 tggcttcacg cgggcgataa tgccctgatg cacggcaccg tcgcttttct cgtcgagata
     1441 ttggcggttt gccaactgga taaccacgcc ctgagactca agggcgtgaa tcagcggtaa
     1501 cagacgttta tcttcacggc cttttaaaat aaagacttcc tgaaaacgtt caggggcgcg
     1561 ctccagcagg gcctgcactg cgtggatgcc gtaaatcatt tcgctcatta atgtactcgt
     1621 tgtttacgtt ttgcctgatg cactacgttt atccggccta catgatctct gcaatatatt
     1681 gaatttgcgt gattatgtag gccggataag gcgttcacgc cgcatccggc atgaacaaag
     1741 cgcattttgt cagcaatcta accctcttct tttaaagagg gtattgatca ctctgccact
     1801 tttttcttcg ccgcacgctt cgctttggtc gctgcagcta ttttctgcgt tttcgccgat
     1861 ggctttttcg cttttctcgc gtctttcttc gccgctttcg gcttcgtttt tttctcaccg
     1921 cagaaggcgc tgtctggctc aaagtttacc tttttaccga cctgacgacg cttgccgcct
     1981 tttttacctg catcgccttt tttcgctttc tcgcgcgccg ttttaccgac gttgcgcggt
     2041 gcgcgttcgc tggagatcag gctaaagtcg attttgcgct cgtccatatt aaccgcttcg
     2101 acgcgaactt ccacgcgatc gcccaggcga taagtctggc cgctggattc ccccatcagg
     2161 cgttgcccta cctggtcaaa gcgatagtag tcattgtcca gcgaagagac atggaccaga
     2221 ccatcaatga acaagtcgtc cagacggacg aagaagccaa agccagtgac gctggaaatt
     2281 acgcctttaa agacgttacc tacctggtcg agcatgaagt cacacttcag ccagtcagcc
     2341 acatcgcgcg ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg acccagttgc
     2401 agcatctctt ccatcgaata atggtagccg ccggtttcag tggtgttgcc ctgatgcccc
     2461 tgctctttcg ccagcagata tttaatggcg cggtgcagcg tcaggtctgg ataacgacga
     2521 atcggcgaag taaagtgcgc ataggactgc aatgccaggc caaagtgacc acggttttct
     2581 ggatcgtaaa tcgcctgttt catcgagcgc agcagcatgg tttgcagcat ttctgcatca
     2641 ggacgatcgg caaccgactc cagcagctcc gcgtagtcac gcggttccgg cttgttaccg
     2701 cccggcagtt ccagccccag ctccgccagc actgaacgga aagaggtaat cgcttcggtg
     2761 ctcggcttgt cgtgaatacg gaacagtgcc ggttctttcg ctttctcaac gaaacgcgcc
     2821 gccgagatat tcgccagaat catgcactct tcaattaatt tgtgcgcgtc gttacgctgg
     2881 gtctgttcga tacgttcaat acggcgttca gcgttgaaaa tgaacttcgc ttcttcgctc
     2941 tcaaatgaga tcccaccgcg ttcttcacgg gctttatcca gcactttata gaggttatgc
     3001 aactcttcga gatgcttaac cagcggggcg tactgctcgc gcagatcctg atcgccctgc
     3061 agaatatgcc agactttggt gtaggtcaga cgcgcgtgag agctcatcac cgcttcgtag
     3121 aatttgtagc ccgtcaggcg gcctttcgac gaaaccgtca tctcgcacac catacacagg
     3181 cggtctacct gcgggttgag cgaacacagg ccgttagaga gcacttccgg cagcatcggg
     3241 ataacctgcg aagggaagta caccgacgtg ccacggttac gcgcttctct gtccagcggc
     3301 gttggcggac gcacatagta gctgacgtcg gcaatcgcga cccataaacg ccagccgccg
     3361 ccgcgttttt tctcgcagta aactgcatcg tcaaagtcac gggcgtcttc gccatcaatg
     3421 gtgaccagcg gtaaatcgcg cagatcaaca cggcccgctt ttgcttcttc cggcacttct
     3481 tctttcagcc cggcaacctg ttgctcaaca gcctgcggcc agatgtacgg aatttcatgg
     3541 gtacgcagag cgatatcaac cgccatgccg gtgcccatat tgtcgcccag cacttcgacg
     3601 attttaccca ccgctttggt gcggcgagtc ggacgctgag tcagttcgac tacgaccaca
     3661 aagcccatcc gcgcgcccat gatctgatcg ggcgggatta agatatcgaa gctcagacgg
     3721 ctatcgtcag gaaccacaaa gccgacgccc gcttcagtaa agtagcgacc aacaatctgg
     3781 ctggtttttg gcaccagtac gcggacaata cgcgcttcac gacgaccttt acggtcagcg
     3841 cccagcggct gcgccagcac ctgatcgcca tgaatgcagg ttttcatctg ctcgctggag
     3901 agatacaaat catctttacg cccttcaacc cgcagaaagc cgtagccatc acggtggcca
     3961 ataacggtac ctttcaccag gtcgaggcgt tccggcagcg catagcactg acgacgagtg
     4021 aagaccagtt gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc ttcaagctgc
     4081 tcttcgcctt caatgtgcag ttctaccgcc agctcatcac ggctggccgg tttttcacgt
     4141 ttggttaaat gttcgaggat aaattcccga ctagggatgg gattcgcgta tttttcagct
     4201 tcgcgttcct ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc tctgatgaag
     4261 attttcgtca ctccaccagc aataatttat aaagcggttg attctcttca accaaatcgg
     4321 caagcgtgta gttatccagt tccataagaa aactttgcac ggccttagaa agcgcctgtt
     4381 tcaaccgaca ggcaggtgta atgtggcaaa actcactgct gcaattcacc agcgataagg
     4441 gctccagctc gcgcaccaca tcaccaatac gtatcgcact cgccgattta cccaggcgaa
     4501 tgccgccatt ttttccacga acagcagtca cgtagccggc acgactaagt tgattgatta
     4561 ttttgaccat atgattacgg gagacgccgt agacgtcagt cacttcagaa atactggtca
     4621 tccgcccttc tggcaatgac gccatgtaga tcagcgcacg taatccgtaa tcagtgaaac
     4681 tcgttaactg cacatcaacc tcaaaaggga aatcgggaaa aaatacattt atattgatga
     4741 taaaccagcc acaagctgtg tcgctaattt atttcagttc ggggaaggaa aaaagcgagg
     4801 atttaacact gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta tcacactgtt
     4861 agcccggcag gcaaaatatc tgccaggcgt accagaatta cgcgtcgaac gggtcgcgca
     4921 gaatcatggt ttcagtacga tccggaccgg tagagatgat atcgatcggc acaccagtca
     4981 gctcttcaat acgcttgata tagttcagcg ccgcctgcgg caggccgcta cgatctttca
     5041 cgccgaaggt ggattcagac cagcccggca tggtttcgta aatcggctct acacctttcc
     5101 agtcgtcagc tgccagcgga gtggtagtca cttcgcgacc atccggcata cggtaagcca
     5161 cgcagagttt aacctctttc aggccatcca gaacgtccag tttagtcagg cagaagccag
     5221 acagggagtt cagctgtacc gcacgacgaa cggcaacggt gtccagccag ccggtacgac
     5281 gacgacgccc cgtagttgcg ccgaattcgt taccctgctt gcagaggaac tcgccagttt
     5341 catcaaacag ttcggtcggg aacggacctg cacctacacg agtggagtaa gctttgagga
     5401 tacccagaac gtaatcaaca taacgcgggc ccaggccgga accggtcgcc acgccaccag
     5461 cagtggtgtt ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc agcagcgtac
     5521 cctgcgcacc ttcaaacatg acgaaatcgc cacgctgacg cgcctggtcg agcaggtcag
     5581 aaacgtcaac caccatagaa gtcaggatgt cggcaacagc catcgtatca tccagaactt
     5641 tctggtaatc aaccgcttca gctttgtagt agttaaccaa ctggaagttg tgatattcca
     5701 tcacttcttt cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca acacgcagac
     5761 cgcgacgtgc tactttatct tcataagcag gcccgatacc acgaccggtg gtgccgatcg
     5821 ctttcgcgcc acgcgctttc tcacgcgcgt tatccagcgc aacgtgataa tcaaggatca
     5881 gcggacatgc ttcagacagc agcagacgct cacgaacggg gatgccacgg tcttccagtt
     5941 ctttcatctc tttcatcagc gcggccggag acagcacaac accgttaccg atgatgctgg
     6001 ttacattctc gcggagaata cctgatggaa taagatggag aacggttttt tcaccgttga
     6061 ttacgagagt atggcctgcg ttgtgaccgc cctggtagcg tacaacatat ttagcccgtt
     6121 cagtcagaag atcgacgatc ttacctttac cttcgtcacc ccattgggtg cccagtacga
     6181 cgacgttgtt acccattttt cagaatcacc gtttgcttaa aaatggattc taccatcgct
     6241 ttttcagagt tacagcactt tttgcactca aaaatgacct cgatcagtct gttttttgtt
     6301 cagccaatcg ttttcctcaa catgtagtag accacaacgc ccgcaaccac aagtccaccg
     6361 ccaaaacgac gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat cttcttccat
     6421 gccttcgggt aaagcatcgg ccctaaacct tccagtacca aaaccagggc aagcgccagc
     6481 cagattgtcg aattcattca ttatccttat agaaaaagaa aaccaccgac atccctgagg
     6541 atgcggtggc tttattgacc tgtaccgcag tcgttatatt aacgcgttgc ggaagtcggc
     6601 gtcttcatat agcggaagaa atcgctatcc gggctcatga ccatcacgtc ctgattgcca
     6661 gagaagctgt tctcataagc acgcaggcta cggatgaatg cgtagaagtc cggatcttta
     6721 ctgaatgcat cagcaaacag tttggctgct tcggcatcgc cttcaccacg catgatacgg
     6781 ccctgacgct cagcttctgc cagcgttctg gtcacttcat agtcggcagt cgcgcgcagt
     6841 ttttccgctt cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc acgctcggcg
     6901 cgcatacggt tgtagatcgc ttcagacact tcggtcggca ggttgatctg cttgatacgc
     6961 acatcgacaa cttcaatacc cagcgccgcc atactgttcg ggttgatgac cggaactttg
     7021 cccttcgtct cagccgttac gcgctctgcc gcttcggcaa tggcgttatc tgctgccggg
     7081 gtagtaactt catcttctgt acccgcagaa ccggagttca gcgcgtcacg tacttcgagg
     7141 gtcagacgac cacgggaatc ggtgacgatg tctttcacgt ccaggcgacc aatttcagaa
     7201 cgcagacggt cagagaactt acgtttcaac agcacttccg cttgcgaaat gtcgccacca
     7261 cccgttgcca ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta agaatcgacg
     7321 atcaggtctt tcttctcttt ggtcacaaag cggtcggcct ggttgtccat ggtctgaata
     7381 cgtgcgtcga gcattttcac cgtttcaatg aacggtatct tgaaatgcag acccggctca
     7441 taaaccagag gtttgttgtc atcgtcacgc agtaccttac caaaacgcag cgtaataccg
     7501 cgctcacctt ctttgacgac aaagacagac atgtaaagca ctaccagcac gatgatgata
     7561 atcgcgataa ctgacttacg catcgttatt ccccctgacg ctggtagtcg ttacgctgcg
     7621 cgttggcgcg gcgttggtcc ataatatcgc cctgactggt ggacgacgtg ttgcttgctc
     7681 cactggttgt ggaggaagag gctggcggca aacgcagcag attgctggca ccgttatcgc
     7741 tcttcgccgc aggggcgtta ccacctttca gcatctggtc taacggcaga accatcaggt
     7801 tgccaccttt atcgttaacc agcactttgc gggtgttacc caacactttt tccatcgtct
     7861 cgatatacag acgctcgcga gtaatttccg gcgcggcttt atattccggc agaagtttag
     7921 caaagcgcgc cacttcaccc tgagcttcca ggatggtctg ggccttgtac gcacgcgcct
     7981 cttcgaggat acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg gtatacgctt
     8041 ctgcttcacg aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg tcaaacgccg
     8101 cttttacttc ttccggcgga cgagcagcct ggaagttgac gtccagcagc gtgataccca
     8161 tgtcatacgg acggatagtc tcttccagtt cgcgctgagt atcgctacga atcacggtac
     8221 gaccttccgt cagaatgcgg tccatggtgt atttaccgat aaccccgcgc agggcgctgt
     8281 cggtagcctg acgcagactg tcatccgggc tggtcacgct atacagatat ttttccggat
     8341 tggtaacgcg gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc gacgtcagca
     8401 tcacaccaga agcggccagt tcacgcacgg cttccacgtt caccggtttg acttcgtcga
     8461 taaacgtcgg tttccagttc agacccggct caaccagatg gctgaattta ccaaagcgtg
     8521 ttaccacgcc gcgttcggct tctttaatgg tatagaaacc actggccgcc cagataatga
     8581 caatcgctgc cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc gggccttgcg
     8641 atgaactgcc accgccagat ccggtgcctt taccgccgcc cagaccaccg agctttttgc
     8701 tcagtttgcg gaagatatca tctaaatcag gtggcccttg atcgcgaccg cctttgtttc
     8761 catttccctc agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg tcttgtccgt
     8821 tattaccggg ctgattccac gccatgtttg tgctccatat ttgttatgcg gtgatccctg
     8881 ttgtctttcg acgccagagg atatgactcc acgcttcaga cgctacgccg ttagatcagg
     8941 taatcgatca acgccggttc ttgtttacag aggcgacgcc agtcaacgat cggcatacga
     9001 acttgcagac ttacgctgcc gtcctcctcc atccactctt tttctattgc ctgaagctga
     9061 taaaaacgac ttctcagacg tccttcctgc ggcggcaaac gcaatgtatg ctgcgccacc
     9121 tcgccggaaa gccgctccgt caaagcctga aaaagctgtg gtatccccgc tccggtctgt
     9181 gcggaaagcc agacacggat cggtttgttc tcttcgtccc gatcaatacg cggttcgaaa
     9241 tcttccagca tatcgatctt gttcatcacc agcagggttg ggatctcgtg agcgtcgatc
     9301 tcttcaagaa ccgtattcac cgcttcgatg ttttcttgta cacgtacatc cgccgcatca
     9361 atgacgtgca gcagtaatgt ggcttgccgc gtctcttgta acgtggcttt aaatgctgcc
     9421 accagatcgt gcggcaggtg gcgaataaac cctacggtat ctgcaagtac ggtttcaccg
     9481 acatctgcaa cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa ctggtctgcc
     9541 gcgtagaccc gcgcttcggt gatgcgattg aaaagggtag atttaccggc gttggtatat
     9601 cccaccagcg aaacagtagg aacgtcggct ttgatacgcg attgccgccc ctgctcacgc
     9661 tgcttttcaa ctctttccag gcgcgactgt atctgcacga tgcgattacg caacaaacga
     9721 cggtcggttt cgagctgggt ttcacccgga ccacgcaaac ctatcccgcc tttctgtctt
     9781 tcaaggtggg tccagccacg caccaggcgc gtagccagat ggcgcagctg cgccagctca
     9841 acctgcaact taccctcatg ggtacgcgca cgttgggcga aaatatctaa aataaggccg
     9901 gtgcggtcga taacacgaca ctcgcacaaa cgctccaggt tacgctcttg cgccgggctc
     9961 agggcatggt caaaaagaac gaccgaagca cccgtcgctt tgacagcttc cgcaatttca
    10021 actgctttac cttcacctac aaaatacttt gggtgcggcg ctttacggct accggtaatc
    10081 acctgcaatg cttcgacacc ggcggaagag accagagatt caaactcctg gaggtcttcc
    10141 atatctttgt cttgcgtaaa atagatgtgt accagtaccg cctgctcacc agcatcataa
    10201 cggtcaaaca agcgtataac cctctaaata gatcagcggg gaacgcagga tcgctggctc
    10261 cccgtgtaaa aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga
    10321 agtattctgc gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt
    10381 actgtgatga gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat
    10441 ctggctgacc gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg
    10501 cagcttaata ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt
    10561 caggaacgga tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat
    10621 ttgtactttg aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca
    10681 ttcagcctgc gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt
    10741 cactgtcaag ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca
    10801 actgtctcgt ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa
    10861 ggtaagacca catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat
    10921 ctcctcgggc aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa
    10981 aacgctgctc aatgcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat
    11041 gcacctgata cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg
    11101 aaatgaaaaa aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg
    11161 ctgccgcaac cggatctacc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg
    11221 ccgcctgttg ctcaattctg gcccgcactt ccgggtctgc cgacggtagc ggcgacaacc
    11281 cttccagcaa tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg
    11341 ccgcggtgat atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag
    11401 cctgcgacgg atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt
    11461 tcggcttcgc cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca
    11521 actctactgg taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg
    11581 gccccatcaa aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg
    11641 gcttttatcg ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca
    11701 agttgcggac ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac
    11761 tgcgcatgtt cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat
    11821 acggactgct tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg
    11881 cgtaagggta aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata
    11941 cccaattccg ccagggcaga ctgcgctttt tctaatgccg atttttcttc gccagaaact
    12001 tttagccgca acggaataag cagcggctga gcgcaaactg gcgcttcacc cggcgtcagt
    12061 tgtacctgac gcagccaacg ttctgccact ggcaaggcta aaagtgaaat gttgccgtcg
    12121 cgctccagca acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg
    12181 ttcgccgcaa gtgcaggttc ctgcggttcc ggcgctttta atttttgcat cggcgcgggc
    12241 gtttgcaaaa gctggcgata cacttcacct tgctgtttct ggtagcctgg ctgcgcattc
    12301 ggccaggggg cagccggacg actgcctgat gctggcgcag gagtgtagcg cggagctacc
    12361 ggctcacgag ctgccggttc tgcaaagtga ttgcgccccg ccgccacgcg gttttccgga
    12421 atggaacgcg gtgcaggttg gggttcatcg tccagcggta gcggcgtttc cagttgctgt
    12481 tgtagcacgc tcagcacgcc ctgatagata aaatcatgca ccagacgcga ctgatggaaa
    12541 cgcacttcgt gtttggcggg gtgcacgttg acgtccacct gatgcggatc gatctccaga
    12601 tacagcacaa atgccggttg ctgatcggcc cccagtttgt cttcgcaggc ctggcggatc
    12661 gcgtgattga tcaggcgatc gcgcatcatg cgaccgttca cgtagcaata ctgaatttct
    12721 gccagtgcgg gcgtggtgtg atttggatcg gccacccagc cgcgtagcgt gagatcgccg
    12781 tgttgccatt caatcgccag cgcttgttca agaaaagcgg tgccgcaaat cgcgcctaag
    12841 cgccgttctt tttgcccgcc ttccggcact gcgcggtact gacgcacaat tttaccgtta
    12901 tgcgacaggt tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcggat gatctcatca
    12961 atgtggttaa attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag
    13021 aacagatcca gcacctccag cgtcgtcccc acaggatgcg ccgccggttt taccgtcacg
    13081 ttcatatcgc gcccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa
    13141 gtgagcgtca ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg
    13201 ctgataatgg cttcgagatc gtccagagag gcgattttac tggtggcatg acgcgccagc
    13261 gccagcgcca gctcgtcttt tttgataccg cagccgttat cacgaatgcg gataagtttc
    13321 gccccaccgc gttcgatatc aatatcgata cgtgtcgcac ctgcatcgag gctgttttcc
    13381 accagttctt tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc
    13441 gccagttgtg gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga
    13501 tctggcgtcg tcaccgtact ggccgtttgt gccgttgccc cctgcggcgc agattgcatc
    13561 ggatgcgcaa ggaaataatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg
    13621 taatcgtcgc tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc
    13681 agtactgatg ggatatccgg cgaacgcaga acgccaaggc tggcatgctc tggccgacgt
    13741 ttatgtattt cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct
    13801 acccgctggg aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcagac
    13861 tgactgttcg ccagcacatc acccgccccg cccagcagct ccgactgttt ctcgtgctgc
    13921 tccagccagc tggccatttc gctgttggcg cgacggttag agagcaccca tacggaagcg
    13981 ccagtcgcac tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt
    14041 tgcttacgtg ccacatcgct gcgccccatc accgagataa agtaatcccc gtcacgggtt
    14101 aaaacgcctt taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg
    14161 atggtgacat ttttctcccg cgtaccaccg gggccgatag cgccagggtc ctgaccgccg
    14221 tgtccggcat caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc
    14281 ggacgcgtta ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac
    14341 ggattgcgcg ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc
    14401 gcaaccacgg gcggtggagg aggcggtggc ggcacatcgg cgttaatcgt aaagacgaca
    14461 gtgtaattgc tgccattctg ccgcttcacc gcttcggttt taccgttttc ggtaagatcg
    14521 accaccagcc gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc cttcaccaga
    14581 ttattgccgc tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc
    14641 gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac
    14701 gttatccgcg cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc
    14761 accggcgtgc acagcagcag cagcgtcgct accaaccaat ttctgatgcg atacatcatc
    14821 ccgccacctt tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa
    14881 accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc
    14941 gggtcaggaa gaacacctgt accttgttgt ggccactcca ccaggcagat ggcatcgttg
    15001 gcaaaataat cgcgaatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa
    15061 tcaaagtgat aaaccattaa attgtcgagc gtatagggtt cgaccagcgt ataagtgggg
    15121 cttttgacat taccctgatg acccagagct tgtaaaaagc cccggctaaa ggtggtttta
    15181 cctgcgccta aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc
    15241 cgctcgccca ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg
    15301 gtttttatca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc
    15361 cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgcagcac cgtgcgcgac
    15421 acagcccgca caggcggcat catacggcga cagtttttgc ccaagcaatg cgccaataat
    15481 accagagagc acatcgccca tgccgccgct cgccatgcct gcatttccgg catcaataat
    15541 gcctaaagcg tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc
    15601 gccataacgt tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac
    15661 ggaacagcct aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg
    15721 cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg
    15781 aaaattctca actttttgca gtgctttttt cccccactct tgctggccca ggccgggacc
    15841 aatcaccacc acatcggccc attccaggct ttcggtaaga gagtccatcg tcagttcatg
    15901 caccatcaat tccggtcgtg cagtcagcag cggcgcaatg ttttcactgc gggtcagtac
    15961 tcggactaaa ccagcaccag cacgcagcgc cgcttccccc gtcatacgaa tagcccccgc
    16021 cgtgccgtga tcgccaccga taattaccag ccgcccgtga tcgcctttat gcgaagtcgg
    16081 gcgacgcggt tttagccagt gagaaagttg ttctgctgaa aaccgctgaa ttttcgtctc
    16141 ctgacctgcc agccaactat ccagccccag tgagtcaaaa tgcagttgtc cggtaacatc
    16201 ccgcgctttt ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc
    16261 gttgatcacc gcgcctggcg tagcgccagt ttcagccagc aggccggaag ggatatcaac
    16321 cgccacaatc ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg
    16381 gggcgcttgc cgcaaaccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg
    16441 ccagacaata ttcgaagcat ggatctcgcc acccgcgttt aaccatgctt cgcgtgccag
    16501 cgtggcctct tccggcaacg gtttgtcgct ctcctgggcc aacaacgtga cctcaatgcc
    16561 gaccgctttg gccagtcgcg cgaccacgta gccatcgccg ccattattac catgaccgca
    16621 cagcaccagc cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc
    16681 gccagcgcga agcatcagct catagagtgt gagccccagc acatctgccg cctcgcgttc
    16741 tccgcggcgg atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg ggtttttctt
    16801 cattgtatgg tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa
    16861 acagtggggg ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga
    16921 gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat
    16981 ggcacgtcac ggtatgctgc gcgctcgccc tcatgagtta ttgcccggta cgctgcgcgt
    17041 gatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa
    17101 ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg
    17161 caaccgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag
    17221 accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg
    17281 ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct tctttttagg
    17341 cgaattgctg gtcgatattc cgctgcccgt ggatcaacca atcgaggaag gatgcggcaa
    17401 atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga
    17461 tgctcgccgc tgtatctctt atctcaccat cgaacttgaa ggggcgatcc cggaagagtt
    17521 gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg
    17581 gaatcgctat tcacaactca ccacagaaga ggatttcagc ccgcgtaagc cgctacacgc
    17641 accggaactc attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg
    17701 atcggcgatt cgtcgtattg gtcacctgcg ttggctgcgt aatatcgccg tagcattagg
    17761 caatgcccct tgggatgaaa cgattttgac agcgctggaa agtcgtaaag gtgagcaccc
    17821 acttctcgat gagcacatag cgtgggcgat tgcgcagcaa atagagagac gaaatgcgtg
    17881 catagtcgaa gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg aaaaagggtt
    17941 accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc
    18001 aagacgaata aaatcgtcaa gtgatcgact cgacaaaacg aaatcttaat ttttaactta
    18061 tctatcaata ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta
    18121 cagcctgagg ctgtggataa ctctgtttac aaaagtttgt cagagacaac gaaaaccagc
    18181 aataacgcat cgcatcgctg tggatatttt attgagagaa gaatttggag cgggaaacga
    18241 gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg
    18301 cttgggtggt ctgtgcctta cagca
//
LOCUS       DAOFQD010000097        18040 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.097, whole genome shotgun sequence.
ACCESSION   DAOFQD010000097 DAOFQD010000000
VERSION     DAOFQD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.097"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            255..836
                     /gene="mdaB"
                     /locus_tag="QBF46_003381"
     CDS             255..836
                     /gene="mdaB"
                     /locus_tag="QBF46_003381"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDQ6675525.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            867..1181
                     /locus_tag="QBF46_003382"
     CDS             867..1181
                     /locus_tag="QBF46_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDQ6675526.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1229..3121)
                     /gene="parE"
                     /locus_tag="QBF46_003383"
     CDS             complement(1229..3121)
                     /gene="parE"
                     /locus_tag="QBF46_003383"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDQ6675527.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3150..3731)
                     /gene="yqiA"
                     /locus_tag="QBF46_003384"
     CDS             complement(3150..3731)
                     /gene="yqiA"
                     /locus_tag="QBF46_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDQ6675528.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3731..4558)
                     /gene="cpdA"
                     /locus_tag="QBF46_003385"
     CDS             complement(3731..4558)
                     /gene="cpdA"
                     /locus_tag="QBF46_003385"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDQ6675529.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4583..5005)
                     /locus_tag="QBF46_003386"
     CDS             complement(4583..5005)
                     /locus_tag="QBF46_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDQ6675530.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5006..5635)
                     /gene="nudF"
                     /locus_tag="QBF46_003387"
     CDS             complement(5006..5635)
                     /gene="nudF"
                     /locus_tag="QBF46_003387"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDQ6675531.1"
                     /translation="MLKPDSLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5840..7321
                     /gene="tolC"
                     /locus_tag="QBF46_003388"
     CDS             5840..7321
                     /gene="tolC"
                     /locus_tag="QBF46_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDQ6675532.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7469..8140
                     /locus_tag="QBF46_003389"
     CDS             7469..8140
                     /locus_tag="QBF46_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDQ6675533.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8146..9306
                     /locus_tag="QBF46_003390"
     CDS             8146..9306
                     /locus_tag="QBF46_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDQ6675534.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9344..10132)
                     /gene="ygiD"
                     /locus_tag="QBF46_003391"
     CDS             complement(9344..10132)
                     /gene="ygiD"
                     /locus_tag="QBF46_003391"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDQ6675535.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10275..11048
                     /gene="zupT"
                     /locus_tag="QBF46_003392"
     CDS             10275..11048
                     /gene="zupT"
                     /locus_tag="QBF46_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDQ6675536.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAVVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11106..11276)
                     /locus_tag="QBF46_003393"
     CDS             complement(11106..11276)
                     /locus_tag="QBF46_003393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675537.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11538..12191)
                     /gene="ribB"
                     /locus_tag="QBF46_003394"
     CDS             complement(11538..12191)
                     /gene="ribB"
                     /locus_tag="QBF46_003394"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDQ6675538.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12295..12443)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12565..12855
                     /gene="ubiK"
                     /locus_tag="QBF46_003395"
     CDS             12565..12855
                     /gene="ubiK"
                     /locus_tag="QBF46_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDQ6675539.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
     gene            13139..13690
                     /locus_tag="QBF46_003396"
     CDS             13139..13690
                     /locus_tag="QBF46_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDQ6675540.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQSDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            13750..16248
                     /locus_tag="QBF46_003397"
     CDS             13750..16248
                     /locus_tag="QBF46_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6675541.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVIIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLSTLINNKRTPIHLDLQWVLIDNLTAVCVTPEQLTL
                     LGFTDEFIEKTQQTLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLATGVGFNLNSLGSLSFDVTRSDAQLHNQNKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVSMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEDDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNNISHTASWYDSSDRNNSWSVSASGDNDEFKDMEASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIIGVIRLPDGSHPPLGISVKDETSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPKSSNLTTGTVILPCIS"
     gene            16270..17019
                     /locus_tag="QBF46_003398"
     CDS             16270..17019
                     /locus_tag="QBF46_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDQ6675542.1"
                     /translation="MRYLNTKNIIAAGVLLSCMNSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKKLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPNEQIQIRMITQEKIAQL
                     PKDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMTYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGVTMLPQTQSLMVLPFSHADTQFKNVPSTFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            17021..17272
                     /locus_tag="QBF46_003399"
     CDS             17021..17272
                     /locus_tag="QBF46_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675543.1"
                     /translation="MRYLLTVIAFIMGFSALPVWAMNCYAEHEGGNTVVIGYVPRIAI
                     PSDGKKGDKIWQSSEYFMNVFCNNALPPHHQEKNTHLHG"
     gene            17278..>18040
                     /locus_tag="QBF46_003400"
     CDS             17278..>18040
                     /locus_tag="QBF46_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675544.1"
                     /translation="MMLLASGQDFYNQNSYTLGVTYNGVDYDSTSTRAIAAPECIDVK
                     GAGIYSNTYKNPAVCSGGPEPQLSVTFPVRVQLYIKLAKNANKVNKKLVLPDEYIALE
                     FKGMSGTGTIDTDKNLTFRIRGLNNIHVLDCFVNVDLEPADGVVDFGKINSRTIKNTS
                     VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDIGNGLLLKIFHNDGTATE
                     FNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVE"
BASE COUNT         4847 a         4503 c         4200 g         4490 t
ORIGIN      
        1 gacatatata ttctccatga ttattttttc cgaaaagctc ctgtatcatt tgcataatac
       61 atctctcctg tgggaactta tgggaagttg atttcagtat tgtcattttc agaaaatatt
      121 tatcctgcat cggtgagtca gagtaagatc agacttttgc taaattcgca aaagactttg
      181 cacattttgc taatttcacc gtaccgctct gtgacgtact atagtcggca aacgtctcac
      241 cttgaggtta aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa ttcgcccact
      301 ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg
      361 ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa
      421 actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt
      481 ggacagtgaa aaaatatatt gatgatgtat tcaccgaagg tcatggaacg ctgtatgcca
      541 gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg
      601 gcaaaaaata tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag
      661 atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc
      721 aattccttgg catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg
      781 atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag
      841 agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc
      901 ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac
      961 tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt
     1021 tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc
     1081 ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg
     1141 tgctggagat gaatatccgc atcctgcaac ctgggattta aacctgcctt gtttgcccgg
     1201 ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct
     1261 cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg
     1321 cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg
     1381 gatcaagcgt ggtttcgcgc aattgcatcg ggttcatttc cccgagacct ttaaaacgct
     1441 ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccttct ttctcttctt
     1501 ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga
     1561 cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca
     1621 gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac
     1681 gaagctggct cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa atatcatgca
     1741 cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt
     1801 tcagtggcat gatcgcctga tattcgcgat cgcgcgcctg cttggcagat ccgcctgcag
     1861 agtcaccttc cacaaggaac agttcggtac ggttaagatc ctgcgcggta cagtcagcca
     1921 gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttc ttagccgcac
     1981 gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt
     2041 tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcact
     2101 gacgcgaaga gagacgctct ttggtctgcc cggcaaactg cggatcctgc atttttactg
     2161 acagcacata ggcgcagcga tcccagatat cttccgccga caactttaca ccgcgcggca
     2221 gaatgttgcg gtattcacag aactcacgca tcgcgtccaa caggccctga cgcagaccgt
     2281 taacgtgcgt accgccctgc atcgttggga taagattgac gtagctttcg gtcagcagtt
     2341 caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatca ccagcgaaat
     2401 taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat
     2461 cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg
     2521 taatctcaac gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa
     2581 cagaaaatcg cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg
     2641 tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa
     2701 aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg
     2761 cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag
     2821 agaatttacc gcccgcgtgc aaacggcaaa gaatcagttc aaccgccggt acaccctctt
     2881 ccgggtgaat atccaccggc atcccgcgcc catcgtcaat cacttctaac gactggtcag
     2941 catgtaagat tacgtccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat
     3001 caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc
     3061 ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca
     3121 tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg
     3181 atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc
     3241 tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc
     3301 tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga
     3361 tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt ctcgttctga
     3421 ccgagatagt ccgtcagcag ttcaaacggg cgtaccgccg ggtttaccac cactgcgggc
     3481 agcataaaac attgcgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata
     3541 cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa
     3601 ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag
     3661 ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga
     3721 agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc
     3781 gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg
     3841 cgatggtgtc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg
     3901 ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca
     3961 gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca
     4021 gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca
     4081 acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc
     4141 tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct
     4201 caccaataaa cacgcgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg
     4261 cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc
     4321 cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga
     4381 caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc cagcaccgcc tggtagcttt
     4441 cccaggtgtt tacccctaac agggcttcgt gcttttgtgc aaacaggtga gtatcggtaa
     4501 tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat
     4561 ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc catcgctcca tgtgctaaac
     4621 agtatcgcaa ccagtccgct aaaaactggt taatttgatg cttttcgtcg cgttgatgca
     4681 actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca
     4741 cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac
     4801 tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa
     4861 tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat
     4921 tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag
     4981 gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca
     5041 gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc
     5101 actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca
     5161 gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct
     5221 cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca
     5281 gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcaa
     5341 tcatcccggc aaccatctcc agtagccagg gggtttcgct ggtgtcgtac gcggcaatcc
     5401 gaatctgctc aatcagcaca acttcatcac gcactgggtc aaagggtagc aagactgcgg
     5461 cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac
     5521 gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa
     5581 taatttctac atcgtttttg ccaaatgtaa cgggcaggct gtctggctta agcattgtta
     5641 atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga
     5701 gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg
     5761 cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc
     5821 ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc
     5881 tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct
     5941 tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa
     6001 tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa
     6061 cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtcct tgcagttaac
     6121 tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg
     6181 gattcaggac gtcacgtatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc
     6241 ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc
     6301 gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac
     6361 cgacgtgcag aacgcccgcg cacagtacga taccgtgctg gcgaacgaag tgaccgcacg
     6421 taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga
     6481 actggctgcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct
     6541 gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct
     6601 ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgactctgg atttaacggc
     6661 ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac
     6721 ccagtatgac gatagcaata tgggccagaa caaagttggc ctgagcttct cgctgccgat
     6781 ttatcagggc ggaatggtta actcgcaggt gaaacaggca cagtacaact ttgtcggtgc
     6841 cagcgagcaa ctggaaagtg cccatcgtag cgtcgtgcag accgtgcgtt cctccttcaa
     6901 caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca
     6961 aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt
     7021 gttggatgcg accaccacgt tgtacaacgc caagcaagag ctggcgaatg cgcgttataa
     7081 ctacctgatt aatcagctga atattaagtc agctctgggt acgttgaacg agcaggatct
     7141 gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc
     7201 gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt
     7261 cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg
     7321 atgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat
     7381 ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg
     7441 tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc
     7501 gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt
     7561 ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga
     7621 cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc
     7681 gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga
     7741 atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca
     7801 ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat
     7861 ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa
     7921 cccagccagt ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc
     7981 ccagccaggc cgcaccatga ccgtaccgaa gacggcaatg gcaccaaaac cggcgaccac
     8041 cactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg
     8101 tagtgcaacc ggtacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt
     8161 attaccgagc gcccggactg gcgtgagaaa gcccacgaat acggtttcaa ttttcacacc
     8221 atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt
     8281 gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa
     8341 gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacacac ctggagtttt
     8401 gtgcgccagt catggctgac gcaccagcca tcgctttatt cgcgtcttga tctggcgtgg
     8461 gatggcactg gtgaacctaa acttctggaa aataacgccg atacgccaac gtcactatac
     8521 gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg
     8581 gagggcagcg accagtttaa cagtctgcaa gaaaaactga tcgatcgctt cgttgagctg
     8641 cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat
     8701 cgcggaacca ttcagtattt gcaggactgc gcaacggaag ctgaaattgc tactgagttc
     8761 ctctacatcg atgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag
     8821 gtaatttcca acctgttcaa actgtatccg tgggaattta tgttgcgtga gatgttctca
     8881 accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc
     8941 aacaaggcac ttctaccgct actgtgggag atgttcccga atcacccgaa cctgctgccc
     9001 gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc
     9061 tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa
     9121 ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc
     9181 gacagctata tgctgattgg tagctggctg gtgaacgatc aacccgccgg aattggcatt
     9241 cgtgaagacc gtgcattgat cacccaggat atgtctcggt tttatccaca tatttttgtt
     9301 gaataagcca cgataccgga tggcactcgc catccggtaa ttgttagcct atctgcaccg
     9361 acagcatact taaactgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc
     9421 catcccacgc acctaacaca tacaacaacg gcagatagtg ctctggcgtt gggttcgata
     9481 acgcgccacc ttcatggtcg agataattca ccagaggatg ctgctctacc ggcccttgcc
     9541 acgtcagatt cgctttcaca tactcattaa acgacgtcgc ccacggatac ggtgaactat
     9601 caccgtgcca cttcactgtg cgcaggttat gcaccacgtt gccactggcg accaacatta
     9661 ttccttcatc tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt
     9721 tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca
     9781 gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg
     9841 gcgctaacag ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg
     9901 tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg
     9961 cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc
    10021 gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt
    10081 tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat
    10141 ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac
    10201 ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt
    10261 ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt
    10321 ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt
    10381 cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg
    10441 cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc
    10501 ttggctattt tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa
    10561 aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca
    10621 tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg
    10681 agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tattcctgaa ggtctggcgg
    10741 ttgcaggccc ggtttatgca gcaacgggtt ctaaacgcac ggcaattctg tgggcgggga
    10801 tttctggatt ggcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga
    10861 tttccccggt ggtcatggcg gcaatcatgg cggtggttgc aggaattatg gtggcgctct
    10921 cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg
    10981 gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa
    11041 ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga
    11101 aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc
    11161 gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg
    11221 aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt
    11281 ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc
    11341 ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa
    11401 aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc
    11461 atagcaggga aaaataaaaa ccgaagcctg ctacaacttc ggtttcttga ttaaggcagt
    11521 aaattaagca gcggttttca gctggcttta cgctcatgtg cctgacggta tgccaccaag
    11581 tcttcaatag tcacgagcgc catgttgtgt ttattggcaa actcaatgca ctctggtgca
    11641 cgcgccatcg tgccatcgtc attagtcagc tcacacagca caccagccgg tttaaagcct
    11701 gccagcgtca tcaaatcaat agtcgcttca gtatgaccgc cacgcgtcag tacaccacct
    11761 gcctgcgcgc gaagtgggaa aacgtggccc gggcgattca gatcagaagg ttttgcgcca
    11821 tcggcaatcg ctgcgcgaac ggtcgtaata cgatcagcgg cagaaacacc ggtagtcaca
    11881 ccttcagccg cttcaatggt cacggtaaaa ccggtgccat aggcgctggt gttattttct
    11941 accatcattg gcagatcaag ttgtttacgg cgatcttcag tgatgcacag gcaaacaata
    12001 ccgctaccgt ggcgaatggt cagcgccatc tgctcaacgg tcatggtttc tgccgggaag
    12061 atcatatcgc cttcgttttc acggtcttca tcatcaagca ccattacacc gcgtccttca
    12121 cgcagcgcag ccagtgcatt ttcaacacgt tcgaaaggcg taccaaaaga ggaaagtagc
    12181 gtctgattca tggtaaaaaa acctcactaa aattatggtt accagaatca gggcagtctt
    12241 aggagtggcg gcatatagcc aaaataacgt gagcgggtcc atgcccgaca gaatcgttac
    12301 tctctcccat ccggactcta accgtcggcc ccggaattac accggatctg ctgtcctttg
    12361 agttcgcact caaagcgctc gcgggctttc aaccaagttg atttaccgcc ggtggggaat
    12421 ttcgccccgc cctgagaata agcggattca ctataacgct aatgattagc ggcagcaacg
    12481 catagcttca cataattctg gtttatgact tacccttatc gcactacaat ggcactcaac
    12541 acctatcagt acagggaaac tacaatgatt gacccgaaaa aaattgagca aatcgctcgc
    12601 caggttcacg aatcaatgcc taaaggaatc agggagttcg gggaagatgt ggagaaaaaa
    12661 atccgccaaa ccctacaagc gcagctgacg cgcctcgacc ttgtaagccg tgaagagttc
    12721 gacgtccaaa cgcaagtcct gttacggact cgtgagaaac tggcgctgct tgaacaaaga
    12781 atcagcgaac tggaaaatcg cagtactgag atcaaaaagc agccagatcc agagactctc
    12841 cccccaacgc tgtaaacatt tgtctttaat gcactgtaaa ttatgaatta atcctctaat
    12901 ttacagtgct ttctattacc ttatcgcgta accccattca gaaaagcatc acaaaaatga
    12961 atgagtgcaa ttgaaaatat aattgttatg tattctttaa gttttactta atcgatattt
    13021 agataaacta attttataaa aatcacaaaa caaaatataa tattttaatg attggtagtc
    13081 cttaaataac agttactaat cattctcttt tttacttatc tttatataaa ggaatattat
    13141 gtctgctttt aaaaagtcac ttcttgttgc tggtgtagca atgattcttt ccaataacgt
    13201 gtttgctgat gaaggtcacg gtattgttaa atttaaaggt gaagttatct ctgctccatg
    13261 ttctattaaa ccaggtgacg aagatttaac tgtaaatctg ggtgaagtag cagataccgt
    13321 actgaaaagc gatcagaaat ctttggcaga acctttcact attcacctgc aggactgcat
    13381 gctgagtcag ggtggaacta cttattcaaa agcgaaggtt actttcacta cagcaaatac
    13441 catgacgggt cagtctgatc ttctgaaaaa taccaaagaa actgaaatcg gcggcgccac
    13501 tggcgttggc gtacgtattc tggacagcca gagcggcgaa gtaactttag gtactccggt
    13561 agtaattacg ttcaacaata ccaacagcta tcaggaactg aatttcaaag ctcgtatgga
    13621 atccccaagc aaagatgcca ccccgggcaa cgtttacgct caggctgact acaagattgc
    13681 ttacgagtaa tcgcatttgg ccgggttcac ccggccacat cattaacgga ttaatgataa
    13741 gtggatcaga tgtataaaaa attaaaatta accacaataa gcgaattgat taaaaatatt
    13801 tattgttcat tatccgttat catcattggt tgtgcgtcag cttatgccgt tgaattcaac
    13861 aaagatttaa tcgaagccga agatcgtgaa aacgttaacc tttcccaatt tgaaactgat
    13921 ggccaattac ccgtcggcaa atattcacta agcactctga ttaataataa gaggacgcca
    13981 atccaccttg acctccaatg ggtattaatt gataacctaa ctgcagtttg cgtgacacca
    14041 gagcaattaa cattattagg atttactgat gaattcattg aaaaaactca gcaaaccctg
    14101 atcgatggtt gttaccctat cgaaaaagaa aaacaaatta caacttatct cgataaaggg
    14161 aaaatgcaat tatccatatc tgcacctcag gcatggttaa aatacaaaga tgcaaactgg
    14221 acgcctcctg aactttggga tcatggtatt gctggggcat ttcttgacta caatttatat
    14281 gcctctcatt atgcaccaca tcagggcgat aattcgcaaa atataagttc ctatgggcag
    14341 gctggggtta atcttggggc ctggcgcctg cgtactgatt accagtacga tcagtcattt
    14401 aacaatggca aaagccaggc gaacaacctg gattttccgc gtatttattt gtttcgccca
    14461 atcccagcaa ttaatgcaaa actaactata ggtcaatacg atactgaatc ctctattttc
    14521 gactctttcc atttttctgg cgtttcgttg aaaagcgatg agaatatgtt gccgcctgat
    14581 ctacgtggtt acgcaccgca aatcacgggt gttgcacaaa cgaatgcaaa ggtcactgtc
    14641 tcacagaaca accgtattat ttatcaagaa aatgttcctc ctggcccatt tgctattact
    14701 aatttattca atacattaca ggggcaactg gacgtcaagg ttgaagaaga ggacgggcaa
    14761 gtcacgcaat ggcaagttgc atctaatagc attccttatc tgacgcgtaa agggcagatt
    14821 cgctacacta ctgcaatggg gaaaccgacc agcgttggcg gcgattcctt acaacagccc
    14881 ttcttctgga ccggtgaatt ctcatggggt tggctgaaca atgtatccct gtatggtggt
    14941 tcagtattaa caaaccgtga ttatcaatcc ctggctaccg gagttggttt taaccttaac
    15001 tcgttgggct cattatcctt tgatgtcacg cgatctgatg ctcagttaca taatcagaat
    15061 aaagaaacgg gttatagcta ccgtgctaac tattcaaaac gttttgaatc taccggtagc
    15121 cagctcactt tcgctggcta ccgtttctct gataaaaact ttgtgtcgat gaatgaatat
    15181 atcaatgaca ctaaccatta cacgaattat cagaatgaaa aagagagtta tattgtcacg
    15241 tttaaccagt atcttgaatc attgagatta aatacatacg taagtctggc tcgtaatact
    15301 tactgggacg ccagcagtaa tgtgaattat tcattatcac ttagccgcga ttttgatatc
    15361 ggcccattaa aaaacgtatc cacctcacta acatttagcc gaataaactg ggaagatgac
    15421 aaccaggatc aactgtacct aaatatttca attccctggg gaaccagtag aacattgagc
    15481 tatggtatgc aacgaaatca ggataacaac atttcgcata ctgcttcgtg gtatgactct
    15541 tccgatcgaa ataattcctg gagcgtttct gcttcaggcg acaatgacga atttaaagat
    15601 atggaggcgt cactgcgcgc cagttatcag cataataccg agaacggtcg tctctatctc
    15661 tccggtacat cacagcggga cagctactat tctctgaatg ccagttggaa cggttcattc
    15721 actgcaactc gccacggcgc cgctttccat gactatagtg gtagtgctga ctcgcgtttt
    15781 atgatcgacg cagacggcgc tgaagatatt ccgttgaata ataaacgcgc ggtaactaat
    15841 cgttatggca tcggagttat tccatcagtc agcagttaca taacaacatc attaagcgtt
    15901 gatacccgaa atctgccaga aaatgtggat atcgaaaact cggttatcac caccacctta
    15961 accgagggtg ctattggcta cgccaaactt gatacccgca agggctacca aatcataggg
    16021 gttattcgcc tgccagatgg tagtcaccca ccactgggga ttagcgtaaa agatgaaacc
    16081 agccacaaag aattaggact ggttgctgat ggcggttttg tatacctcaa cggcattcag
    16141 gatgacagca aacttacttt acgctggggt gacaaatctt gctttattca accacccaag
    16201 agcagcaact taaccaccgg aacggttatt ttaccgtgta ttagctaaaa ttaattcagg
    16261 atgaaactaa tgcgctactt aaatacaaag aatatcattg cagcaggagt attactctcc
    16321 tgcatgaaca gcattgcctg gggtgccatc atcccggatc gtacccgtat aataatgaat
    16381 gagtcagata aaggcgaagc attaaaactt actaatcaaa gtaaaaaact gccctatctg
    16441 gcccaaacat ggatagaaga tacgaaaggc aataaatctc gtgactttat tgttacggtt
    16501 cctcctatgg tgcgtttaaa tcccaatgag caaattcaga ttagaatgat aactcaggaa
    16561 aaaattgctc aacttcctaa ggacagagaa actctgttct attttaacgt gcgagaaata
    16621 ccgccaaaaa cagataaaaa aaatgtgatg caagtgacta tgcagcacgc tttgaaatta
    16681 ttctggcggc caaaagccat tgagttagaa gacgatgggg taatgactta cgaaaaagtt
    16741 gaaatcatcc gtagaaatga tggttcgatc cgctttaaca ataaaatgcc ttatcacgtt
    16801 acgctgggtt atatcggaac aaatggggtc acgatgttac cgcaaacgca aagcttaatg
    16861 gtcctccctt ttagccatgc agatacgcag tttaaaaatg ttccatcaac ttttcaggtt
    16921 ggttatatca atgattttgg tggcttaagt ttttatgaaa taaactgccc aacagtgaat
    16981 aattcttgta atgtttccgt agccaaacga gataaataaa atgcgctact tattaactgt
    17041 aattgcattt attatgggtt ttagtgcgtt acctgtatgg gcgatgaact gctatgctga
    17101 acatgaaggg gggaataccg tagtcatagg ctacgtacca agaattgcta tccccagcga
    17161 tggtaaaaaa ggtgataaaa tctggcaaag cagtgaatat tttatgaatg tcttctgtaa
    17221 taatgcacta cccccccatc accaggagaa gaatacccat ctgcatgggt aaatataatg
    17281 atgttgttag catcaggtca ggacttttat aatcaaaact catatactct tggtgtaacc
    17341 tataatggag tcgattatga ttcaacctcc acacgagcta tcgcagcacc agagtgcatt
    17401 gatgttaaag gcgcagggat atacagtaat acctataaaa accccgctgt ctgtagcggt
    17461 ggtcctgaac ctcaattgtc agtaactttt ccagtacgtg tacagctgta tattaagctg
    17521 gctaaaaatg ctaataaagt aaataaaaaa cttgtattac ctgacgaata tatcgccctg
    17581 gagtttaaag gtatgagtgg cacaggaact atagacacag acaaaaattt gaccttcaga
    17641 attcgtgggt taaataatat tcatgtcctt gactgctttg ttaatgttga tctggaacca
    17701 gctgatggcg ttgtcgactt tggtaaaata aattcccgaa caattaaaaa taccagcgtg
    17761 agtgagacgt ttagcgtagt catgaccaaa gatccgggtg cggcctgtac tgagcagttt
    17821 aatattttag ggagtttttt cactacggat attttgagtg attatagcca tctggatata
    17881 ggtaatggtc tgctattgaa gatatttcat aacgatggaa cagcaacgga atttaaccgc
    17941 ttctcacaat ttgcttcttt ttcatcgtct agtgcgcctt cggtcaccgc accattcagg
    18001 gcagaactga gtgcgaaccc ggcagaaacg gttgttgagg
//
LOCUS       DAOFQD010000098        17449 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.098, whole genome shotgun sequence.
ACCESSION   DAOFQD010000098 DAOFQD010000000
VERSION     DAOFQD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.098"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            202..456
                     /gene="bssS"
                     /locus_tag="QBF46_003401"
     CDS             202..456
                     /gene="bssS"
                     /locus_tag="QBF46_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDQ6675545.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            571..1689
                     /gene="solA"
                     /locus_tag="QBF46_003402"
     CDS             571..1689
                     /gene="solA"
                     /locus_tag="QBF46_003402"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDQ6675546.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1737..1850
                     /locus_tag="QBF46_003403"
     CDS             1737..1850
                     /locus_tag="QBF46_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDQ6675547.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2111..2677
                     /locus_tag="QBF46_003404"
     CDS             2111..2677
                     /locus_tag="QBF46_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDQ6675548.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2681..3256
                     /locus_tag="QBF46_003405"
     CDS             2681..3256
                     /locus_tag="QBF46_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDQ6675549.1"
                     /translation="MKKSLLGLTFASLMCSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEINPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3298..4350)
                     /locus_tag="QBF46_003406"
     CDS             complement(3298..4350)
                     /locus_tag="QBF46_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDQ6675550.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARRACEQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPACAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4575..5495
                     /gene="lpxL"
                     /locus_tag="QBF46_003407"
     CDS             4575..5495
                     /gene="lpxL"
                     /locus_tag="QBF46_003407"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDQ6675551.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5667..6893
                     /gene="mdtG"
                     /locus_tag="QBF46_003408"
     CDS             5667..6893
                     /gene="mdtG"
                     /locus_tag="QBF46_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDQ6675552.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDVGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6976..7350
                     /locus_tag="QBF46_003409"
     CDS             6976..7350
                     /locus_tag="QBF46_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDQ6675553.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7351..7578)
                     /locus_tag="QBF46_003410"
     CDS             complement(7351..7578)
                     /locus_tag="QBF46_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDQ6675554.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7751..10294)
                     /gene="mdoH"
                     /locus_tag="QBF46_003411"
     CDS             complement(7751..10294)
                     /gene="mdoH"
                     /locus_tag="QBF46_003411"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDQ6675555.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWERED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYFSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10287..11822)
                     /gene="mdoG"
                     /locus_tag="QBF46_003412"
     CDS             complement(10287..11822)
                     /gene="mdoG"
                     /locus_tag="QBF46_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDQ6675556.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12216..13373
                     /gene="mdoC"
                     /locus_tag="QBF46_003413"
     CDS             12216..13373
                     /gene="mdoC"
                     /locus_tag="QBF46_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDQ6675557.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13381..14862)
                     /gene="clsC"
                     /locus_tag="QBF46_003414"
     CDS             complement(13381..14862)
                     /gene="clsC"
                     /locus_tag="QBF46_003414"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDQ6675558.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14804..15337)
                     /gene="ymdB"
                     /locus_tag="QBF46_003415"
     CDS             complement(14804..15337)
                     /gene="ymdB"
                     /locus_tag="QBF46_003415"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDQ6675559.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVEANSFTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15432..15743)
                     /locus_tag="QBF46_003416"
     CDS             complement(15432..15743)
                     /locus_tag="QBF46_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6675560.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTVQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15864..16196)
                     /gene="csgC"
                     /locus_tag="QBF46_003417"
     CDS             complement(15864..16196)
                     /gene="csgC"
                     /locus_tag="QBF46_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDQ6675561.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16255..16710)
                     /gene="csgA"
                     /locus_tag="QBF46_003418"
     CDS             complement(16255..16710)
                     /gene="csgA"
                     /locus_tag="QBF46_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDQ6675562.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16751..17206)
                     /gene="csgB"
                     /locus_tag="QBF46_003419"
     CDS             complement(16751..17206)
                     /gene="csgB"
                     /locus_tag="QBF46_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDQ6675563.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4391 a         4426 c         4120 g         4512 t
ORIGIN      
        1 aataattgca caatatgcta aaaaaatatg aatgacatgc tgttttattg ttcatataaa
       61 tcttaaaagc agaaatttat ctgttccccc ctttaaaaat tcggtgaata cccttactta
      121 ttggtatact gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt
      181 aacacgaatt agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc
      241 gtagggtggg acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac
      301 cagaccccaa ataagtccga gcaggaaggg actgaagttg gtcagacgct ctggttaacc
      361 actgatgttg ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc
      421 ggtgatttcc cggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa
      481 ggcacctcac cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc
      541 tgcttaaacc atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc
      601 ggttccgtag gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg
      661 accgacgccc atatgccacc gcatcaacac ggcagccacc acggcgatac gcgattaatt
      721 cgccatgctt atggtgaagg cgaaaagtat gtcccactgg tactccgcgc gcaaacactg
      781 tgggatgagc tctcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt
      841 aacctcggcc cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg
      901 caactcaacg tcgaaaagct cgacgcgcaa gggattatgg cccgctggcc agaaatacgc
      961 gtcccggaca actacatcgg cttatttgag actgattccg gttttttgcg cagcgaactg
     1021 gcgattaaaa cctggatcca actggcgaag gaagcgggct gtgcgcagct gttcaactgc
     1081 ccggtcaccg caattcgtca tgacgatgat ggcgtaacta ttgaaacggc tgacggtgag
     1141 tatcaggcga aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag
     1201 ctgcctgtcc agcccgtacg caaagtattt gcctggtatc aggccgatgg tcgctatagc
     1261 gtgaagaata aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt
     1321 tttccggcag aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat
     1381 tcagcggatg aacgtgttcc gtttgcggaa gtggtcagcg atggttcgga agccttcccg
     1441 ttcttgcgca atgtcttgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat
     1501 gataattcgc ctgacgaaga ttttattatc gataccctac ccggtcacga taatacactg
     1561 ctcattaccg gcctgagcgg gcacggtttt aaatttgcgt ctgttttagg ggaaatagct
     1621 gccgattttg cgcaagacaa aaaaagcgat tttgatttaa cgccattcag gctttcccgc
     1681 ttccaataat catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc
     1741 gtcggctgtt gcactatctc atcaataata ttcgcgagca tctgatgctt tatctttttc
     1801 tctggggatt gttggcaatt atggatctga tctatgtgtt ttatttttag ttttccgcac
     1861 tgacataaac tgacaaatat ttaatctccc tttcatttag cgtgaaaaat tgaatgagat
     1921 tattcctgct tctacagcac tcaccagaca tctctgatgc tgaacccact aaacttatga
     1981 agattctttc attattaatt caaaattatt gaaataaaaa taaatatcat tcaatttgtt
     2041 gtttttgtgt tgcaattcat tctccgacac gccattttct ttctctcgca aattcgtatt
     2101 ggatgcactt atgtcattca caaatacccc tgaacgctat ggtgttatat ctgcggcctt
     2161 ccattggcta agcgcaatta ttgtctacgg catgtttgct ttaggtctgt ggatggtcac
     2221 gctcagttat tacgatggct ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat
     2281 tttgttaatg atggggctgg ttattcgcgt tctgtggcgt gtcatatctc caccgccggg
     2341 tccgctgccg agttattcgc ccatgactcg tcttgccgcg aaagctggac atctcgcatt
     2401 gtacctattg ctgttcgcca tcggcatcag cggctatctg atctcaactg ccgatggtaa
     2461 accgatcagc gtttttggct ggtttgacgt ccccgcgacc cttgccgacg ctggcgcaca
     2521 ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag tactgtccgt
     2581 tatgcacgga tttatggccc tgaagcatca tttcatcgat aaagacgaca ctctgaagcg
     2641 catgctggga aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg
     2701 tttaaccttc gcgtccctga tgtgctctgc cggttcagcg gttgccgccg attacaaaat
     2761 tgacaaagaa ggtcagcacg cctttgttaa tttccgcatc cagcaccttg gctatagctg
     2821 gttatacggc acctttaaag atttcgacgg tacttttacc tttgatgaaa taaatccggc
     2881 tgccgataaa gtgaatgtga caattaacac caccagcgtc gatactaatc acgccgaacg
     2941 cgataaacat cttcgcagtg cagatttcct caataccaca aaatatccac aggcaacatt
     3001 cacctccacc agcgtgaaga aagacggtga cgaactggat attaccggcg atctgacact
     3061 gaatggcgta accaaacccg tcacgctgga agcgaaatta attggtcagg gcgacgaccc
     3121 gtggggcggt aaacgtgcag gctttgaggc cgaaggcaaa attaagctca aagacttcaa
     3181 tatcaagaca gatttaggtc cagcttctca ggaagtggat ctgattattt cagtggaagg
     3241 cgtacagcag aagtaagcgt gaaatgttgc cggatggcac gcatccggca atgatattta
     3301 ttctgttgga tcaggaatgc ccagtgttgt attcagacgt ccacgagact tattaaagat
     3361 cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct
     3421 ttcttcgcag caaatctcac tacaacaacc tttgtatttt tccgcacatg ctggacactg
     3481 aataaacagc aggtggcagc catcattttt acagttggta tggctgtcgc acggcgcacc
     3541 gcactggtgg caatgcgcga taatctcatc cgatatacgt tcgcccatcc gctcgtcaaa
     3601 aacaaaattt ttgccaataa aacgcaccgg caagccctgc tcgcacgccc tacgggcgta
     3661 ttcaataatt ccaccctcga tatgccagac tttattgaat ccgttatgtt tcatccaggc
     3721 actggccttt tcacaacgaa tgccgccggt gcagtacatg acgatttttt tatctttatg
     3781 tgcctgcatc atctcgactg cttttggcag ctgctcacgg aaggtatctg ccggaatttc
     3841 gagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt cgataaacag
     3901 tgcatcggga tcgtcaagca tggcgttcac ttccgccgct tgcagatact caccaacatt
     3961 gctggcatca aagtgaggat cgtcaatacc gtcggcaacg atacgatcgc ggaccttcat
     4021 gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg atattcaggc gtaagccatc
     4081 taaagccgag tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac
     4141 gctgatttgc gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa cattcagcgc
     4201 ggtaaacagc tgatataaag cgtcacgggt cgccttagga tcggcgatgt ggaaatactt
     4261 gtaaaacgaa atggtggttc gcggttcgct ctcagccaac attttggctt ttagcgcgtc
     4321 gttggaaatg cggttgtgta acactggcat ggtgtacggt tcctgcgaga tgggaaagta
     4381 aaaatccgcg gcatgatata gcaattatcg ataattaaca tccacacatt ttacgctaca
     4441 tttgcgcatt aaaaattatt tgttatttac aagcgcggca atttcgccca gtcttcagcc
     4501 acaaattttg gttgcgggcg aaaaaatgcg acaatacata caattgcccg aataggttga
     4561 aaaacaggat tgatatgacg aatctaccca agttctccac cgcactgctt catccgcgtt
     4621 attggttaac ctggttgggt attggcgtac tttggttagt cgtgcaattg ccctacccgg
     4681 ttatctaccg cctcggttgt ggattaggaa aactggcgtt acgttttatg aaacgacgcg
     4741 caaaaattgt gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa caagaacgcc
     4801 gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa accggcatgg
     4861 cgtggttctg gccggaccgc cgaatcgccc gctggacgga agtgatcggc atggaacaca
     4921 ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt tggcatccat tttctgacac
     4981 tggagctggg tgcgcggcag tttggtatgc aggaaccggg gattggcgtt taccgcccga
     5041 acgataatcc actgattgac tggctacaaa cctggggccg tttgcgctca aataaatcga
     5101 tgctcgaccg caaagattta aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct
     5161 ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt tttcgtcccg ttgtttgccg
     5221 ttgagcaggc tgcgaccacg accggaacct ggatgctggc acggatgtcc ggcgcatgtc
     5281 tggtgccctt cgttccacgc cgtaagccag atggcaaagg gtatcaattg attatgctgc
     5341 cgccagagtg ttctccgcca ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag
     5401 tggtcgaaaa atgcatcatg atggcaccag agcagtatat gtggttacac cgtcgcttta
     5461 aaacacgccc ggaaggcgtt ccttcacgct attaaatctc ccatgccgga tgcttcagaa
     5521 tggcatccgg cattaccaca gcaaatcccc ctgatttagc gataaaagct ctctggattg
     5581 cgccccctgg aagtcgggcg cataattagt gtgcttatct tttcttctta tgttcaccgc
     5641 gcctggcgca ccaacagcgg attgctatgt caccctgtga aaatgacacc cctataaact
     5701 ggaaacgaaa cctgatcgtc gcctggctag gctgttttct taccggggcc gccttcagtc
     5761 tggtaatgcc cttcttaccc ctctacgttg agcagcttgg tgttaccggc cactccgccc
     5821 tgaatatgtg gtccggtatt gtcttcagca ttacattttt attttcggcc atcgcctcac
     5881 cgttttgggg tggactcgcc gaccgtaaag gccgaaaact catgctatta cgctctgctc
     5941 tcggcatggg catcgtaatg gtgttgatgg gactggcaca aaatatctgg cagtttttga
     6001 tcctacgggc gcttcttggg ttacttggcg gatttgtccc caacgctaat gctcttatcg
     6061 ccacacaagt accgcgtaat aaaagcggct gggcgctggg tacgctctcc acaggcggcg
     6121 ttagtggtgc gttgctcggc ccaatggctg gcggcctgct cgccgatagc tacggcttac
     6181 gtccggtatt ctttattacc gctagtgtgc tcatactctg ctttttcgtc accctgtttt
     6241 gcatcagaga aaaattccag ccggtcagca aaaaagagat gctgcacatg cgggaagtgg
     6301 tgacatcact taaaaacccg aaactggtac tcagcctgtt tgtcactacg ttaatcatcc
     6361 aggtggcgac gggctcaatt gcccccattc tgacgctgta tgtccgcgaa ctggcgggta
     6421 acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc ggtgccaggc gtggcggctc
     6481 tgctaagtgc accacgactc ggcaaacttg gcgatcgaat cggacccgaa aagatcctga
     6541 ttacagcgct gatcttttct gtactgctgt tgatcccaat gtcttacgtt cagacgccat
     6601 tgcaacttgg gattttacgt tttttgctcg gtgccgccga tggtgcacta ctccccgccg
     6661 tacagacatt gttggtttac aactcgagca accagatcgc cgggcgtatc ttcagctata
     6721 accaatcgtt tcgtgatgtt ggcaacgtta ccggaccatt gatgggagca gcgatttcag
     6781 cgaactacgg tttcagagcg gtatttctcg tcaccgctgg cgtagtgtta ttcaacgcag
     6841 tctattcatg gaacagtcta cgtcgtcgtc gaatacccca ggtatcgaac tgatttttcg
     6901 cctttcatac ttgcaaaagc ggagaatcag ctatcctttt ccctgaaacc tcatcaactc
     6961 aaagggagaa tcgtgatgac catgtacgca acgcttgaag aagccattga cgctgcacgc
     7021 gaagaatttc ttgcagacaa ccccggcatc gacgccgaag atgcgaatgt gcaacagttc
     7081 aatgcccaaa aatacgtttt gcaggacggc gacatcatgt ggcaagttga gttttttgcc
     7141 gacgaagggg aagaaggtga atgtttacct atgcttagcg gtgaagccgc gcaaagtgtt
     7201 tttgatggcg actatgatga gatagagata cgccaggagt ggcaggaaga gaatacatta
     7261 catgaatggg acgaggggga atttcagctt gagccaccgc tggataccga ggaaggacgc
     7321 gcagcagctg atgagtggga tgaacgttaa tcactcatac gggccatgat gaatgtcgat
     7381 cggcagcagt aaagtatcaa aaaccaggga gaatggcaga tcaaggattg tgacataacg
     7441 ccaggcggag tcacgcacat cccattgcac accgggataa tattggttgc catgcccctg
     7501 ccccggtata gtgcgactaa taatgctgcc acagccactc agcaaggtca ccatgatgct
     7561 caccacaatt aaacgcattt gatacctctt ccgtaaaaaa ataccggcat aacgccggta
     7621 tttttattgg ctcgttgttt ttgccggatg cggcgtgaac gccttaccca acctacaaaa
     7681 tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag cgcatcaggc
     7741 aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga ttgagcttta
     7801 tcccttcgta ataactcacc catgaaaaat atctctccgg ggaattccag acgcggaaat
     7861 gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg cgattcagct
     7921 tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt tccagcacct
     7981 tactggcgcg gtgacgtgcg gtcgccattg cggttgccag agcgttaaat gacgggttaa
     8041 acaccgcgtg cataaaacca tcatcaaggg agcgttgacg attcatctca aggaagcgat
     8101 cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac agtttccagc
     8161 gtttggttcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac ggtgacagga
     8221 tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccaac cacgccatac
     8281 caacagccca cactaaccct aacagcagct gtgagccgtg gcgtttgaac gcttcacccc
     8341 aggaagtgga gtcatcatca cgctgcggtg aattccacac aacttcccag ccaaggaacg
     8401 cgctgacgac gaagaccgta tggaacagca tgcgtaccgg agccagcagc acggaaaaca
     8461 gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct ttcgttcctt
     8521 tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc atggtcgaag
     8581 caaaaagtgc aatcgccagc tcaggacgcc actgcggcca taccgggaac aactgccgtg
     8641 gttgcaggaa gtattgcggt tcggtcaacg catggactac ctgcaatgca gtagagagcg
     8701 cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc aggaacaccg
     8761 cacggtgaac cgggtgcata cccttcacca ggaacagacg gaagttcatc aggttaccgt
     8821 ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat tcttcataag
     8881 aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc aacgccgctt
     8941 ccacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc agcggagcca
     9001 gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc gttatgtccc cagtagtgcg
     9061 actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg
     9121 cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc gacgactgaa
     9181 tgatcccggc gttcgggttg gcttccatca ggcgcaccag cccgcacaaa caatcaccgg
     9241 tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc cagcgacggc
     9301 agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg cgatagaaaa
     9361 tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc tctgcgacgc
     9421 agatatccgg gttataactg tcactaagaa tgtagacatc aaagtgtttg gcattcccgg
     9481 tggcttttac tgattcccac gttgcacgca ggccagcaaa aacacggttc acgtcttcgt
     9541 tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca tcgccaactg
     9601 ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag cccattaacg
     9661 ccgtccagaa tccggcggac acccaacaga acagtaccgc aaagaggatc aggataccgg
     9721 tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga ccaaccatat
     9781 ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata taccaggtcg
     9841 cgacgacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg gtaccgacgg
     9901 tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga tagcgcggcg
     9961 tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta cgccacgggt
    10021 cgggaaacat cgaggatcgt tttgcttctg gcatcgcctt cagctgatcg cgcccttcgt
    10081 cgtctttaat taactgtcca tcagcaagtg agtctggcca ggcttgttcc agacgcgcct
    10141 ttaccgagcc ttgcggggaa tcatcctccc gctcccaggt gcggtgttcg gcatccagcg
    10201 cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt gcggcgatgg
    10261 gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta gctccaggtt
    10321 tcactcaacg tctgatcggc attcaccagc gcagctcgca tttcagtggt tttcttggca
    10381 tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt atagcgcacc
    10441 gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac cggggtatcc
    10501 tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat agtaccgtca
    10561 ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc
    10621 catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat ggtgtattta
    10681 aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc gacgatgtta
    10741 tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt gccccactcc
    10801 cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag atcttcaaag
    10861 cgggagaaat cacgaccgcg ctgcaacaga ccaaagcctt gcgggttttc catggagaag
    10921 ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc gccattaccg
    10981 gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt tgcaggcgac
    11041 ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag tttgccgact
    11101 ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc tggcataact
    11161 acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca atgcataaat ggttaaacgt
    11221 ttatcagttg gttttggacg ctcgatccag aactctttga agcgtggaaa ttcttcaccc
    11281 gatggcaagg cggtatcaat tgccagaccg cgggcagaaa ggccataaac ctgacctgca
    11341 ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt tttatctttg
    11401 ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac cgtgtctttg
    11461 tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg tttgactgcg
    11521 gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg atggtagaac
    11581 tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt aaactggatc
    11641 tgctgatagt ccgcgtattt catatcgcgg aaaacggagg gcaagttgct tttgggcgcc
    11701 tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat actgaaagcc
    11761 cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg cattttcatc
    11821 atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct tatatcgatc
    11881 cgatctattt taatggataa ccagccattc cgacaacaga atcccggtat cgttcagcac
    11941 gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc ttctcctaaa
    12001 gacagaagca tggacttcgt gtagggtttg ccagcaaatt tatttatcaa ccgtcttata
    12061 gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct tgaagcctgc
    12121 gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata acccgacagg
    12181 gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc gcgcaacgtg
    12241 aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct tttcatattt
    12301 ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca ttatggctga
    12361 ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt atatccggct
    12421 acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa gtacgtgtcg
    12481 aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattgccg caatttatta
    12541 tgctgcaata cgtcaaaggg aaagcggaaa gttggcctgg gctgtcattg tatgacaaat
    12601 ataatacgtt agcctgggaa ttaatatcac acctgtggtt tttactggtg ttagtggtca
    12661 tgacgacgct gtgcgtatgg atatttaaac gcatcagaaa taatttagaa aattctgata
    12721 aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttgtgc ctcggcatcg
    12781 gttatgcggt aataagaaga acgattttta ttgtgtatcc gcccattctg agtaatggca
    12841 tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc ctcggcgcac
    12901 tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt ggctgtaccc
    12961 tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc agtggcgatg
    13021 cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggtctgtgg atggtgaatg
    13081 tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg acttattttg
    13141 ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt ttcggcgcat
    13201 acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg atattcgtag
    13261 tagggattgc gataattctg tatgaaattc atttgcgcat cccgttactg aagtttttgt
    13321 tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt taagccacgc
    13381 ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc gcttccagaa
    13441 actggtggcg ggttcttttt tgagaataat ctctttcttt gcatgacgat caacccagtt
    13501 gatccgtccc cacctgtcca gacggagctg ccaggccgca tcatactggc tctgaataaa
    13561 gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca tttcagtatt
    13621 gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt taccatcgat
    13681 gctaaaggtt ttagcatgca gactggcacc ggaattacct gttatgccgc gatcgtgtaa
    13741 cgtactactt tgttcacgcg tcggcttgag ttcatataat tccacgccat aacggagcaa
    13801 ttttttgcgc caccgcgcgt atccggcatg gacgacagca acatcgttag cggcaagaga
    13861 attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt gcgccacacc
    13921 tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg gtgagcccat
    13981 gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct cccctttcgc
    14041 cggatcatca cttaataaac gtgttttcgc ccagataagc ggcaacgttc catcaaccag
    14101 atgatttata aacggactgg attccatttg gcgtaagtaa cgatgcgtca tggcatcgtt
    14161 atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga catccagcac
    14221 ctgctgtaag ggtgaaaccg atttgcaata ccagtaacgg gcgaaatcat cggcaacgtc
    14281 ctctaccacg ggtcctattg ccataacatc taaatccgaa aaaagtggct cttcccctgc
    14341 cccaaaatag gcatcaccaa tatttcgtcc tcccaccagg gtaaccacgc catcgacagt
    14401 gaaactttta ttgtgcatac ggcgattgag acgggaaaag tcggtgatat aaccaagcgg
    14461 acgcagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcggg gatggctgtc
    14521 aagcaagcgt aaaatatcgt caagtccggg cgtattgttg tcgtccagca acaaacggac
    14581 gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgac ccgacatatc
    14641 gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg ccagacgata
    14701 ccgggctgca aacgcatcca gacttttctc cagaggaaaa aggccacact gaccgggatg
    14761 ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca tctccttgtt
    14821 gagtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca aagtatacct
    14881 gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt ttttactgcg atttcagccg
    14941 ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaaagcc actgacgtaa
    15001 agctgtttgc ctccaccagt cgtaggctat tgagataggc atcctgcaaa agctggtctt
    15061 cattttgttc accaccacgc cagactggcc cgacggtgtg cactacggct ttagcaggaa
    15121 gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc tgttgcctga
    15181 cttttaaaca agcatccagc agggccggac ccgctgcgcg atgaatggcc ccatcgacgc
    15241 cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg gccagtttgg
    15301 taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac accttcttgt
    15361 ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag caacgtgagt
    15421 tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg tttatcaaga
    15481 tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat taacgctttg
    15541 ctggagtagg ttttgcccgg aatagaacca ttctgtgggc agttcatttc tacgggtgat
    15601 gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg gatcacgccc
    15661 ccttgcacgg tgcttccagc aatggcaata aaaggaaaaa acaaactgcc gagcataaga
    15721 gagtttaaaa aaggtctgaa catttattct atttcctcaa tgattagtca tccttgaggg
    15781 ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct ccagattcat
    15841 cttatgctcg atatttcaac aaattaagac ttttctgaag agggcggcca ttgttgtgat
    15901 aaatgaagtg actgtccatc agaaacagta acaactattt tcacccgatc gtccggggaa
    15961 atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga aagggtcttt
    16021 tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat ttgtactctg
    16081 cacagacaag attgagtaag agtgacttca ggaataatgg tatacacatc cccttgctgg
    16141 gtcgtattaa aggttatctg actggaaagt gccgcaagga gtaataacgc attcatattc
    16201 ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga tgtattagta
    16261 ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg aggagttaga
    16321 tgcagtctgg tcaactgcag caccgttgcc accaccgaac tgtttaaccg tcatttcaga
    16381 atttttgccg ttccactgat caagagtagc gctgttaccg aagccacgtt gggtcagatc
    16441 gattgagctg tcatccgagc cctgaccaac atctgcacca ttaccgccgc catgctgggt
    16501 aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt taccgccacc
    16561 gtactggtaa atgttcagct cagaatttgg gccgctatta ttaccgccac caccgtggtt
    16621 accgccgccg ccgtactgag gaacaacacc tgccagagcg ctaccggaga atacgattgc
    16681 tgcaattgct gctactttta aaagtttcat gtaaaacccc catcggattg atttaaaagt
    16741 cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg cgactgtctc tgcactacaa
    16801 ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt attaccagaa cctttctgga
    16861 taatcatcgc agtattacca taagcacctt gcgaaatact ggcatcgttg gcactgcccg
    16921 cctgatcaat atatgcaagg ttataatctc ctgtctggtc aatctttgcc cggttgctac
    16981 taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc ctgccgtaac tgagcactat
    17041 tattagtccc agcttgacca attatggctg cctgattaaa tgaagactta ctcaattcat
    17101 ttaccgcgaa gttatattct gaattagcta aatcataacc tgctgcggct gcaatcccag
    17161 gcgcacccag tattgttaac atcataaata acaatttgtt tttcatgttg tcaccctgga
    17221 cctggtcgta catttaagaa attaaatcat ttcaacttgg ttgttaacgc aacctgtatt
    17281 ttgttaacgc tgcgttacga tggaaagtat gtctgcggaa atatttttaa taactcaccc
    17341 gcgcgttgta tttttctttt ttagttcata ccaaagtatt aaaacccacg taaatacgct
    17401 gttatctacg caaaaaaata ttttgttttc ttttaaatct ccgttttcc
//
LOCUS       DAOFQD010000099        17440 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.099, whole genome shotgun sequence.
ACCESSION   DAOFQD010000099 DAOFQD010000000
VERSION     DAOFQD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.099"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            337..2238
                     /locus_tag="QBF46_003420"
     CDS             337..2238
                     /locus_tag="QBF46_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDQ6675564.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            2266..2727
                     /locus_tag="QBF46_003421"
     CDS             2266..2727
                     /locus_tag="QBF46_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HDQ6675565.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDKGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            2747..4480
                     /locus_tag="QBF46_003422"
                     /pseudo
     CDS             2747..4480
                     /locus_tag="QBF46_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            4478..6553
                     /gene="nrfB"
                     /locus_tag="QBF46_003423"
                     /pseudo
     CDS             4478..6553
                     /gene="nrfB"
                     /locus_tag="QBF46_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            6540..9512
                     /gene="nfrA"
                     /locus_tag="QBF46_003424"
     CDS             6540..9512
                     /gene="nfrA"
                     /locus_tag="QBF46_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDQ6675566.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLVRLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            9513..10403
                     /locus_tag="QBF46_003425"
     CDS             9513..10403
                     /locus_tag="QBF46_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDQ6675567.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            10586..11347
                     /gene="envY"
                     /locus_tag="QBF46_003426"
     CDS             10586..11347
                     /gene="envY"
                     /locus_tag="QBF46_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDQ6675568.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(11392..11468)
                     /locus_tag="QBF46_003427"
     tRNA            complement(11392..11468)
                     /locus_tag="QBF46_003427"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11432..11434),aa:Arg,seq:tct)
     gene            11711..12343
                     /gene="fimZ"
                     /locus_tag="QBF46_003428"
     CDS             11711..12343
                     /gene="fimZ"
                     /locus_tag="QBF46_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDQ6675569.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(12346..12861)
                     /gene="sfmF"
                     /locus_tag="QBF46_003429"
     CDS             complement(12346..12861)
                     /gene="sfmF"
                     /locus_tag="QBF46_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDQ6675570.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTKEVDANGNVTLNFFANYRALASGVQPGVA
                     KADAIFMINYN"
     gene            complement(12872..13879)
                     /gene="fimH"
                     /locus_tag="QBF46_003430"
     CDS             complement(12872..13879)
                     /gene="fimH"
                     /locus_tag="QBF46_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDQ6675571.1"
                     /translation="MKIICRLLLAMACLWLTNISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(13892..16501)
                     /locus_tag="QBF46_003431"
     CDS             complement(13892..16501)
                     /locus_tag="QBF46_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDQ6675572.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSKQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(16532..17224)
                     /gene="fimC"
                     /locus_tag="QBF46_003432"
     CDS             complement(16532..17224)
                     /gene="fimC"
                     /locus_tag="QBF46_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDQ6675573.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
BASE COUNT         4474 a         4451 c         4406 g         4109 t
ORIGIN      
        1 atatttctta tgcaatcatt cattttaata atgttaattg gattatttat tgctgtgaat
       61 aaaaatatat tatgaatatt ggtttaactt tgcgtttttg ttactcgttg tgatatgcgt
      121 acctgtcctg aataacgcca agaaacaaga ggttgagctt gtgattattt ttttgactga
      181 acagacaccc gtttctgtaa agtaatattt aaacaaaatt ctcacataac catagcattt
      241 tctgttcctt ccctaataac gtctgaagtc gtcccgctta tcacattatc cgcagttgta
      301 tggtacggat cattattcat tttactggag gttgctatgt caaccggatt acgtttcaca
      361 ctggaagtgg acggcctgcc gccggatgct tttgcggtgg tttcctttca tctgaaccag
      421 tcactctctt cgctcttttc cctcgatctc tccctggtca gccagcagtt tctctccctt
      481 gaatttgcgc aggtgctgga caaaatggcc tacctgacga tatggcaggg cgatgaagta
      541 cagcgccggg tgaaaggcgt ggtgacctgg tttgaactcg gggagaacga caaaaaccag
      601 atgctgtaca gcatgaaggt gcacccgccg ctgtggcgtg ccggtctgcg ccagaacttc
      661 cgtatcttcc agaacgagga catcaaaagc atcctcggca cgatgttgca ggaaaacggg
      721 gtgaccgaat ggagtccgct gttcagcgag ccgcatcctt cccgtgagtt ttgtgtccag
      781 tacggtgaga ctgattacga ttttctgtgc cggatggcgg cggaggaagg catcttcttt
      841 tatgaggagc atgcttacaa aagtaccgac cagagcctgg tgctgtgcga cacagtccgc
      901 catctgcccg aatcttttga aatcccatgg aacccgaaca cccgtaccga ggtgagcacc
      961 ctctgcatca gccagttccg ctacagcgca caaatccgcc cttcttccgt ggtgaccaaa
     1021 gactacacct ttaaacgccc cggctgggcc ggacgttttg aacaggaagg ccagcaccag
     1081 gactaccagc gcacgcagta tgaagtgtat gactaccccg gacgtttcaa gagcgcccac
     1141 gggcagaact ttgcccgctg gcagatggac ggctggcgaa acaacgcaga aaccgcgcgg
     1201 ggaatgagcc gctcgccgga gatatggccg ggacggcgaa ttgtgctgac ggggcatccg
     1261 caggcgaacc tgaaccggga atggcaggtg gtggcaagtg aactgcacgg cgaacagcca
     1321 caggcggtgc caggacggca gggagcgggg acggcgctgg agaaccattt tgcggtgatc
     1381 ccggcagaca gaacatggcg accacagccg ttgctgaaac cgctggtcga cggcccgcag
     1441 agcgctgtcg tgacaggacc ggcaggcgag gaaatcttct gcgacgaaca tggtcgcgtg
     1501 cgggtgaagt tcaactggga ccgttataac ccggcagacc aagacagttc gtgctggatc
     1561 cgtgtggcac aggcgtgggc aggcaccggt tttggccacc tggcgatacc gcgtgtgggt
     1621 caggaggtga ttgtagactt cctcaacggc gatccggacc agccgatcat tatggggcgc
     1681 acctaccacc aggaaaaccg cacccccggc agcctgccgg gaacaaagac gcagatgacc
     1741 atccgctcca aaacgtacat gggcagcgga tttaatgagc tgaagtttga tgatgcgaca
     1801 gggagagaac aggtctacat ccacgcgcag aagaacatgg ataccgaagt gctcaacgac
     1861 cgtaccacca ccgtaaaaca cgatcaccgc gaaaccgtaa aaaatgacca gacggtcacg
     1921 atccaggaag gtaaccgcct tcttacggtg gaaaaaggcc acaagatcac cggagtactg
     1981 aaagggtctt tatctgagga tgtctttcag gacagaggca cgattgccgg ttcggtgcat
     2041 gttgacgctg taaacaatgg tggcgaaggc gacggtatac aggcttatac ggcgattaag
     2101 gaaattttgc tggctgtgga ggaaagcaaa attgcgctga cgccggatgg cattcagcta
     2161 caggtcgggg aatcgacggt aatcaggctg tcgaaggatg gcatcaccat cgtgggcggt
     2221 tctgttttca tcaactgatt cactcagatt atcagggagt attttatgca gttcactttt
     2281 aatgaaggcc acattcaact gccgtctcaa tggcaggatc agtcaatgca ggtcctggtg
     2341 tccacagata acagtggcat caacctggtc attacccgcg aagccgttcc acagggaacc
     2401 ctgacaccag agctgtacca ggaaacgctg gcgctgtacc agggcaaact tgatgggtac
     2461 accgaacatg cctgccggga aatcactctg gcagaggccc cggcctggct gctggattat
     2521 tcctggaacg gtccggaaga taaggggaat caggggcgga ttagccagat agccgtattc
     2581 cagcggcgtg gagatacgct gctgaccttc actttcagca cctccttatc cctgaagaac
     2641 agtcaaaaga cgatgctgct ggaggtgata aagagcttca ccccgttgcc accggagaat
     2701 gacatccaaa aggaccagcc gcgctgacat aaacgaggga taacacatga gtgaaggacc
     2761 aggcgggcca cagggagcga ccgcaggcgg tacgctggca atgcgaatgc tgtcacagca
     2821 ggcgatggtc gccagccaga tgaaacgggc agccaacgac aaagccattg cacagatgct
     2881 ggcagcaaag aagtccggcc cacctgccgc caggctgggc gatgaaattc agcataaaag
     2941 ttttctgggg gcactggcag gggccgtgct gggggcgata gtgaccatcg cagaaggttg
     3001 cctgattatg gccgcctgcg ccaccggccc ttatgcgctg gttctgctgc ctgcgctgat
     3061 gtatgccagc tataaggcga gtgattatgt ggaggagaaa cagaaccagc ttgagtcatg
     3121 gataaacagc ttttgtgaca cggacggcgc catcaatacc ggttctgaaa atgtaaacat
     3181 taacggaaag cccgctgcca gggccgccgt cacccttccc cctcctcccc cacctggagc
     3241 aatacctgaa gtcccacagg gggaaccctc atggggtgat attgccactg acctgcttga
     3301 atcggcagcg gaaaaagcag taccactggc gaaggcctgg gggaacgctg ttatcaccct
     3361 gacggacagc aatgccggtt ttatggatcg cgtcagcgcc ggcgcatcgc ttctgtttcc
     3421 cgccggtccg gtattaatgg agtttgccac catggtgggc gggcgtggcg aaatcaaaaa
     3481 agatgtggat ttcccggaag ccggtgagga cacggcgctc tgcgacaagg agaacaaacc
     3541 accgaggata gcccagggca gcagcaacgt ctttatcaac aatcagcctg ccgcgcgcaa
     3601 gggcgacaaa ctggagtgca gtgcggcaat cgtggaaggt tcgccggacg tctttattgg
     3661 cggtgagcag gtcacatatc tggatatcca gctggagttc ccgccatggc agagaatgat
     3721 cctgggagga ataacgatag ccagctatct tctgccgcca gcaggactgc tgggaaaact
     3781 ggggaatctg gcgagactcg gcaaactggg aaacctgctg gggaaaagcg ggaagctgct
     3841 gggcgcaaag ctcggcgcgt tgctggggaa aacaggtaag tcgttaaaaa gtattgccaa
     3901 taaagtcatc agatgggtaa cagatcctgt cgatccggta accggcgcat actgcgacga
     3961 acgtaccgac tttacgctgg gccagaccct ccccctctcc ttcacccgtt tccacagttc
     4021 tgtactgccg ctgcatggcc tgaccggcgt gggctggagc gactcctgga gcgaatacgc
     4081 ctgggtgcgt gaacagggaa accgggtgga tatcatcacc cagggagcca cgctgagatt
     4141 tgccttcgac ggtgacagtg atacggcggt taacccgtat cacgcccagt acattctgcg
     4201 ccgccgcgac gattacctgg agctgttcga cagggatgca ctgagcagcc gcttctttta
     4261 tgacgccttt ccgggaatgc gtctgcgcca cccggtgact gacgatacca gcgatgaccg
     4321 cctggcacac agccccggtg accggatgta tatgctgggc gggatgagtg acaccgccag
     4381 caaccgcatc acgtttgagc gcgacagcca gtaccggatc acgggtgtca gtcacaccga
     4441 cgggatccgg cttaaactga cgtaccatgc cagcggctac ccgcgaatga gttaccgcga
     4501 actgtataaa ccagatgaaa aaccgttagc gattatggtt ccggcatgga atgaaacggg
     4561 cgtcatcggc aatatggccg agctggcggc gaccacgctc gactacgaaa actatcatat
     4621 ctttgttggc acctacccca acgaccccga tactcagcgt gatgttgacg aagtgtgcgc
     4681 tcgcttcccg aatgtgcata aggtagtctg cgcgcgtcct ggccccacca gcaaagccga
     4741 ctgtctgaac aacgtgctgg acgccatcac ccaatttgag cgtagcgcca atttcgcttt
     4801 tgctggtttt attctgcatg acgccgaaga tgtgatttca ccgatggaat tgcgtctgtt
     4861 caactatctg gtcgagcgta aagatctgat tcagatcccg gtgtatccgt tcgaacgcga
     4921 atggacgcac ttcaccagca tgacttacat tgatgagttt tcagagctgc atggcaaaga
     4981 tgttccggtg cgtgaagccc tcgccggaca agtgcccagc gcaggcgtcg gcacctgttt
     5041 cagccgccgc gccgtgaccg ctctgttagc tgacggtgac ggtattgctt tcgacgtgca
     5101 gagtctgact gaagattacg acattggttt ccgcctgaaa gagaaaggta tgacggaaat
     5161 ttttgtccgt tttccggtgg tggacgaagc caaagaacgc gagcagcgta aatttttaca
     5221 gcacgcacgg acgtcaaaca tgatctgcgt gcgcgaatat ttccccgata ccttttcgac
     5281 tgcagttcga caaaaatctc gctggatcat cggcattgtt ttccagggct ttaaaaccca
     5341 caaatggacc tccagcctga cgctgaacta ctttctctgg cgcgaccgca aaggggcaat
     5401 cagtaacttt gtcagcttcc tcgcgatgct ggtgatgctc cagcttttgc tgttgctggc
     5461 gtatgaaagt ttgtggcccg atgcctggca tttcctttct atttttagcg gcagcgcatg
     5521 gttaatgacc ctgctgtggc taaactttgg cttgatggtt aaccgcatcg tgcagcgggt
     5581 gattttcgtc actggctact acggtctgac gcaggggctg ctatctgtcc tgcgtctttt
     5641 ctggggcaac ctgattaact ttatggccaa ctggcgcgcg ctaaaacagg tacttcaaca
     5701 cggcgatcca cgtcgcgtcg cgtgggataa aacaacgcat gacttcccca gcgtcactgg
     5761 cgatacccgc tcgttgcgcc cgttaggtca aattctgctg gaaaatcagg tcatcactga
     5821 agaacaactc gatacagcac tgcgtaatcg cgtcgaaggt ctacgcctgg gcggttcaat
     5881 gctgatgcag gggctgatta gcgccgagca gctggcacag gcgctggcag agcaaaacgg
     5941 cgtggcgtgg gaatccatcg atgcctggca gatcccttcc tcgctgattg ccgaaatgcc
     6001 ggcctccgtg gcgctgcatt atgcagtact gccgctgcgt ctggaaaatg atgagttaat
     6061 tgtcggcagt gaagatggta ttgaccctgt ttcgctggcg gccctgacgc gtaaagtcgg
     6121 acgcaaagtg cgttacgtca ttgttctgcg gggacaaatt gtcacggggt tacgtcactg
     6181 gtatgcacgc cgacgcggtc acgatccgcg ggcaatgttg tacaatgcgg ttcagcatca
     6241 gtggctcacg gaacagcaga ccggtgaaat ctggcggcaa tatgtgccgc atcagttcct
     6301 gttcgccgaa atactgacca cgctcggtca tattaatcgt tcagcaatta acgtgttgtt
     6361 attgcgccat gaacgcagtt ctctgccgct cggcaagttt ttggtcaccg aaggcgttat
     6421 cagccaggaa acgttggatc gcgtcctgac aattcaacgc gaattacaag tttcgatgca
     6481 atcactatta ctcaaagcag gtttaaacac agaacaggtt gcgcaactgg agtccgaaaa
     6541 tgaaggagaa taaccttaat cgcgtcatcg gatggtctgg tttactgctg acgtctttat
     6601 tgagtaccag cgcactcgca gacaatatcg gcaccagcgc agaagagctg gggctgagcg
     6661 attatcgcca ttttgttatt tatccccgtc tcgataaggc gctgaaggca cagaaaaata
     6721 acgacgaagc aaccgccatc cgcgaatttg aatatataca ccagcaggtg ccggataata
     6781 ttccgctgac tttatacctt gcggaagcct atcgccattt tggtcatgat gaccgggcgc
     6841 ggctgttgct ggaggatcaa ctgaaacgtc acccaggaga tgcccgactt gagcgcagtc
     6901 tggcggctat tccagttgaa gtgaaaagcg ttacgactgt tgaagaactg cttgcccagc
     6961 aaaaagcgtg cgatgctgcg ccgaccctgc gttgtcgcag tgaagtcggg cagaatgccc
     7021 tgcggctggc acagttacct gtcgccagag cgcaactgaa cgatgcgacg tttgctgcat
     7081 cgccggaagg aaaaacgctg cgaaccgatc tgctgcaacg ggcaatctac ctgaaacaat
     7141 ggtcccaggc agatacgcta tacaatgaag cacgccagca gaacacatta agcgcggcag
     7201 aacgccgtca gtggtttgac gtgcttcttg ccgggcagct ggacgatcgg atcctggcac
     7261 tgcaatcaca ggggatcttc accgatcctc agtcatatat tacttacgcg accgcgctgg
     7321 cttatcgtgg cgaaaaagca cgcctccagc attatctcat tgaaaataag ccgctgttta
     7381 ccacggacgc acaagagaaa agttggctct atctgttatc taaatacagc gccaaccccg
     7441 ttcaggcatt ggcgaattat acggtgcagt ttgccgataa ccgccagtat gttgttggcg
     7501 cgacgctacc ggtgctgtta aaagaaggtc agtacgacgc agcgcaaaaa ctgctcgcca
     7561 ccctccccgc caatgaaatg cttgaggagc gttatgctgt cagcgtggcg acccgtaaca
     7621 aggctgaatc tctgcgtctg gtacgattgc tgtatcagca agaaccggca aatcttaccc
     7681 gcctggatca actgacctgg caactgatgc agaacgagca gtcacgcgaa gctgccgatt
     7741 tattgctgca acgctatcct ttccagggcg atgcgcgtgt cagccagact ttaatggcgc
     7801 gactggcgtc tctgctggaa agtcatcctt acctggcaac tccagcgaag gtggcgattt
     7861 tatcaaaacc cttgccgctg gcggcgcaac gtcagtggca aagccagttg ccgggtattg
     7921 cagataattg cccggcaata gttcgcttgc tgggcgatat gtcgccttcc tacgatgccg
     7981 ccgcctggaa ccgtctggca aagtgttatc gggacacgct acccggtgtg gccttgtatg
     8041 catggcttca ggccgaacaa cgacaaccga acgcctggca acatcgtgcg gtagcctatc
     8101 aggcgtatca ggttgaggac tacgccaccg cactggcggc ctggcagaaa atcagtcttc
     8161 acgacatgag caatgaggat ctgcttgctg ctgccaatac cgcccaggcg gcaggaaatg
     8221 gtgcagctcg cgatcgctgg ctacaacagg cagaacaacg tggactggga aacaatgccc
     8281 tctactggtg gctgcatgcg caacgttaca ttcctggtca gccggaactc gcactgagcg
     8341 atctcacgcg ctcaatcaat attgcgcctt ctgccaacgc ttacgttgcg cgggcgacaa
     8401 tttatcgcca acgtcataat gtcccggcgg cggtgagtga tttgcgcgcc gcgctggaac
     8461 tggaaccgaa taatagcaac atccaggcag cgcttggtta cgccttgtgg gatagcggtg
     8521 atatcgcaca gtcgcgggaa atgctcgaac aggcgcataa agggcttccg gacgatccgg
     8581 cactgatccg acaactggcc tacgtgaacc agcgtctgga tgacatgcct gcgacgcagc
     8641 actacgcccg gctggtgatt gatgacattg ataatcaggc gctgataacc ccactgaccc
     8701 cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc ggtcgccgct
     8761 ggacgttcag tttcgattct tctatcggct tgcgttccgg ggcaatgagc accgctaaca
     8821 ataatgtcgg cggcgcagcg ccagggaaaa gctatcgtag ctacgggcaa ctggaagccg
     8881 agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt tatagccgcg
     8941 tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg atgtccggca
     9001 ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc gaacagcagt
     9061 tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct gcgcgccagc gcctcattct
     9121 ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg tttgcccaaa
     9181 acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg acggcagatt
     9241 atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct tacgctcacg
     9301 ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc ggagtcgggg
     9361 tccgctggaa tatctggacc ggcgagacgc actacgacgc ctggccgcac aaagtcagtc
     9421 tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga gagcgcaaca
     9481 acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc gtattgctga
     9541 cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg caaccacaaa
     9601 accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag ttacgtttgc
     9661 aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattc acccagccag
     9721 aacagcgcgc gttattgttt aagcgtgccg cagctgcgca acaggctggc ctgaagctga
     9781 ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg tccgcagcgc
     9841 tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga ttatggagcg
     9901 ccgcgcctgg cgtaacgccg gatggctggt acatcagcgc ggaaattgac gacctgaact
     9961 ggcgcagcga agctgcccgt cagcctttgc taacatggtt aaacaacgcg cagcggctga
    10021 ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga aacatgtcgc
    10081 ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggcgttaat gtctgggtac
    10141 aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat ctacaggcca
    10201 gcgccgattg ccaaagtccc gcccctgcca gcggcattgt ttatgaactg tttgtcgccg
    10261 gcaaaggcaa aacctttaca gcgaaaccga aaccggatgc agagatcgcc tcgctgttag
    10321 cgaaacgttc ctcttgcggt aaagacactc tctatttctc tctgcgctat ttgcccgtcg
    10381 cgcacggcat tctcgagtat taaatctcct ccaggtaagt cgggtacgac ctggcttacc
    10441 tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcgtgc taaagaaaga
    10501 agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc tttgagcgga
    10561 gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt gcgtggtgat
    10621 cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta cccttccgaa
    10681 aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa cgttaaatca
    10741 cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt ttttaaataa
    10801 gaacttaacc tgtgtaaagc cctggtcgcg gctggctacc ccggttatcg cttgtcatag
    10861 ccgtacaccg gaagtgttcc ggctagccgc caaccacagc aagcagcaac ccagcaaacc
    10921 ctgcgaagcg gagttgacgc gcgcattgct ttttaccgta ttgtctaact ttcttgagca
    10981 atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg acagcgtctg
    11041 ccgcattatt caaagcgata ttcagcacta ctggaatctg cgaattgtcg ccagttcgct
    11101 atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca gctatagcca
    11161 gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg ataacaaaaa
    11221 tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcgtacttta tctctgtttt
    11281 taaggcgttt tacggcctga caccgttgaa ttatctcgcc aaacagcgac aaaaagtgat
    11341 gtggtgaaga gcaaagcgga acggataaga cggggttaaa tgaagaagaa atggcgcgcc
    11401 ctgcaggatt cgaacctgcg gcccacgact tagaaggtcg ttgctctatc caactgagct
    11461 aagggcgcgt tgataccgca atgcggtgta atcgcgtgaa ttatacggtc aacccttgct
    11521 gagtcaatgg cttttgatct ggttgctgaa caagtgaacg accgcgtctg attttctgat
    11581 ttatttcgct atagcggcaa acaaacgcac accgctgcgc gtctgaatca agaaaacccg
    11641 tattttcatg tatcaaagta caatttaccg ccctaacgga aaattgtccg ctcctatgag
    11701 actggtaact atgaaaccaa cgtcggtgat cattatggat actcatccta tcatcagaat
    11761 gtctattgaa gttctgttgc aaaaaaacag tgaattgcag attgtcctga aaacggatga
    11821 ttaccgcata accatcgatt atctccgaac ccgtcctgtt gatttaatca ttatggatat
    11881 agacttgccc ggtacagacg gttttacctt cctgaaaagg atcaaacaaa tccagagtac
    11941 agtgaaagta ttatttttat catcgaaatc agaatgcttt tatgctggca gagcgataca
    12001 agctggtgct aacggttttg tcagtaaatg caatgatcag aatgatattt ttcatgccgt
    12061 tcagatgatc ctctccggat acacgttttt tcctagcgaa acgctcaact atataaaaag
    12121 caataaatgt agtacgaata gttcaacggt cactgtgcta tctaatcgtg aagtgaccat
    12181 attacgttat ctggttagcg gattatctaa taaagaaatt gccgataagt tattacttag
    12241 caataaaaca gttagtgcgc ataaatctaa tatttatggc aagctaggtt tgcattcaat
    12301 tgtagagctt atcgactacg ccaaattata cgaattaata taatattaat tataattgat
    12361 cataaatatc gcatccgctt tcgccacacc tggctgaaca ccgctggcta acgctcgata
    12421 attggcaaaa aagtttagtg tgacattgcc atttgcatct acttccttag tcgggctcgc
    12481 ctcccccagt gcgagccggg agcgatcgct attacgtaat tcgatggcga cggtttgcgc
    12541 cattgcggga tcatccagag ccagcaggtt ggtatcggat gccggcgttc ccgtaaataa
    12601 aatcgcaact gaacccggag gacatccctc cagccgcagg ctaaaaggga cgagtgccgt
    12661 ggtatcgcca gcgttcagta attgtgtcgt aggccatctg cctaaatcta ccgtcttatc
    12721 aatatccgca gtgtttacgg tacaggagaa atcaacaacg ttaccgtgca aattgatatt
    12781 aatcgttccc aaagggtcag ctgcccatcc actggaactc cacagtaacc cgcagaaaca
    12841 gctaaagagt actcttctca ttctccgtac ctcattgata atcgacgcgt aaatacccca
    12901 gcgcgctaaa cggcccttcg gtcggttttt gaccggtaat actgataggc caggcgcgaa
    12961 gtgtgacatt ggctgccgca gctgcatcca gacggaaagg aataacgcta ttgagatcgt
    13021 taggcgtgat cggcgtatcg ttctgatcgg cgacaataaa acctaaatcc tgattgtccg
    13081 acaccatcgc ctgaccagaa acggcactgg cttccagacg cattgttaaa taagcctgcg
    13141 cagcaacatt cgtacatttg accgcaatgc tcttggtttg cggcatgaca ccagcaggtc
    13201 gattacccgg ccctgccgca ctaaataacg atgcgccgat atcaccaaaa tcaaattcaa
    13261 caatctgccc ggcatttaat tcgcagtttt gcggtacttc aacccggcca ccaaaactaa
    13321 tggtataaac aggtgtaacc aacggatcgt aaggcgttga tgtcacatac acggtaaaca
    13381 tcacctgtct ggggatctcc accatgttaa taaagggacg aataaccttt aaacgaaata
    13441 ttaaacgaga atccatcacc ccgaatggaa ggttttgcga aacgttagga tctgttccca
    13501 tccgaatata attttgcggg gggaactgaa tatccataac actatcaacc agactcatcg
    13561 cacctaatag ataatcattt aattgcatat atttataatt atcgatagtt tcctgaacaa
    13621 taaagttggt gacataacta cggtatgtat aattcaccag cgtgccgggt ggacaaattg
    13681 ctgacacacc tacccagccg gatttttccg gtaaaacaac aatctgccct ggctggttat
    13741 tggtgctatt aaagacattt gagagatcat agtgttcatc ttctgctacg ccagtactat
    13801 ttgcacaaac agtagcccag gatatgtttg ttaaccacaa acatgccatc gccaataata
    13861 atctgcagat tatcttcatt gataatttcc tttatgagcc aggatgtgtg caaactgccg
    13921 aaataactgt gacggcttgc tgtaagcttt gcttcggcaa tacatagtgc gcaatgcagc
    13981 gtgagttcgc gccgtctccc cactgaacca gtaattcacc agacaatggc gcaccactta
    14041 aataaacttg cccgtcatca cccaccatac tggttattcc ggtactgttt tcccgtacca
    14101 gtgatccaaa cggcaccggt tttctgccct gggtaacggt aatgagcgcc cgcacgccta
    14161 tgcgggtatc aaaattggca cgaaccaacg cgccttgcgt cggcacaacg ctgctaatat
    14221 ttttttcaac atcgatggaa ttccccatcg tattggtatc aagcgcgata cggttatacc
    14281 gataaaccgt ggcatacggc atcaccgcat agccgcgcca gtcggttaaa atgccagttt
    14341 gattttcaat gcgtacaccg cctgcgccag gcgctttaat caaaacattg gtatccccta
    14401 aaggctggct cagcgttatg ccattttcat gtccgaccac accgccagac agctgccagt
    14461 taacgtcatg ttgatcgcgg tcgtagttat acccgactcc cagcgtaccg taagcggcct
    14521 gccagtttgc cgtggcgctg cccgtatacc cattattcga ggtatcaccc tggcttacgt
    14581 gataactcag gttgtggcct tccagtaagg tcccacctac acctgccagc cagctatttt
    14641 gcccgttgct gttacggttg gcgttaaagg aggcataagc gcggtcgagc gcattttccc
    14701 gggtgtagcg acgtttggtc aaaacattga aaggcactga aacattaagt ccgacaatac
    14761 gttcgttatc ggggatccct acagattcat tccacgaaaa tgagagcgaa taactgatgc
    14821 caacccagct gctggtatac cccacctgat accacgtatc tgaatccgaa gtattccagt
    14881 atttttgatg ggtaccagaa atataaagcg agccaaagtc attaagtgat tgtgaaatat
    14941 ttaactgcaa acggtcttta cggctaaagc gtaaattgtg gtaattcacg attatcggtt
    15001 catcccgatg ctcaccgtca taatcgtaat catattcgta cccctccatt cgacgatacg
    15061 caacatcatc aagggtataa aaaccttgtg tcgaatagcg gtaacccatt aactgaaaat
    15121 tggtaccgaa ggtgttcatc gatttcgcat agaggaagcg aatggaatcc ccctcatgac
    15181 gactgtcgtc ggctaactgg ctgcgcgcat gggtcacatc cagcgacact gcgccccagt
    15241 tcccgaggtt gcgccccagt cctaataaaa aggcggtgta attggcagat aattgcgtcc
    15301 cgccgtaggc tgtaaattcc tgtggtaaac caccgagtgc cgtgccctga aaaaagaaag
    15361 gtgatgattg ctgactatta ccgctacgaa aatcgcccgc cgtcaggtca aatttgaaac
    15421 gcccttcgcg ttgtaaaatc ggcactgttg aatacggaat tgtgtaattc tgctgattgc
    15481 catcgcgctc gtcgatggtg acatccagat cgccatttga agatgtcggg tgcaaatcag
    15541 taatttcaaa agcaccgggg gaaacatagc tttgatagat aataaaacca ttttgccgaa
    15601 tcgttagctg ggccgccgta cgggcaatcc cacgtaccgt tggggcaaac ccttgctggc
    15661 tatcaggata catattatcg gaagaataaa gccgtacacc acgaaatcca acaccatcga
    15721 aaatatcact tcctgtattg ccgtctccca ttaccagttc acttttcagc ggaataatgg
    15781 cgcgctgtac ccaggtgcca atattattcc actgttttga atgatatcca tttccgcgaa
    15841 aatagttcca ggaaccattg ttgcgtaaac gccacgggcc aatattaatc ccgctgagct
    15901 cactaaaaaa atagctatcg ttaccattac ctctgttacc ggtgaaatta taattcagga
    15961 gtaaagcagg aataccttca tcccactctt caggcggaat gtaaccgtga gcgctactca
    16021 acaacgttat ctgaggaata gtgatgttca ggcgcatcgc tgcaaaatca aagttaattg
    16081 tggcatcagg tacagctttc agtaaattga tgcatttatt gttttgctgc tgggctaact
    16141 cgggaaatgc actgctatta aggccaattc gttcaagaag tacctggtta aaacagggca
    16201 ttaacccacc tgatttatca cctgtatttt ctgttgtcga ttcaaatacg atatcctgcg
    16261 aaccaatgaa ctcatcatta cgccagagat ccacccgata cacgcccgca ggttgatgat
    16321 tacctctctc aaagcgcgat aaatcagcaa cggatgtgcc attctctaat aaaaatgccg
    16381 ggttgaaata actttccgca tagcttaaaa aggagggtaa aatggcaaga gatgaataac
    16441 aaattccggc cagacaccag gtatacctct gcggaatatc cgtagtagtg ggtattttca
    16501 taattagctc tgatattgtc agatgattcg gttagtttaa gttcacttct ctgaccggag
    16561 ttactgaacc ataatcatta acggtctgaa atttcagctt tccctgcact cctgagggta
    16621 agggaatttg tgaattaact ctgggtgcag ccatagcatt ccccaacttt tggctgccca
    16681 cttgtaagtt aaccagggtg acataaaaag gtgatggatt atgaacattg atatagttac
    16741 cggatcgcga aaacttgagt gtgtccggcg cttctgcggg taattcttgt aattgaattg
    16801 gacggagaaa taatttcatg cgcgataaaa tcgccagttg caaaacattc ctgccgttca
    16861 atgcattttt atctaccgaa gggatcgtct taacattcat ccagaacaga gactcacgat
    16921 ctgctgccag cggtggaccg gtgtaaataa tacgcaaagt attttcgctt ttgggttcac
    16981 taacaaacag tggcggtgta atgatgaatg acttttcttt tacaccgctg ctgttttcaa
    17041 tccacgaatt gaccagaaag cgctcattgg tatggctatt tctaatccat accgcagtct
    17101 gtttagcatc agcgggataa ataatacgcg tggcacctaa tgcgatccct ccggcagcat
    17161 gggcgctggc cgaaatgatt aaataaaata taatgagcat caataacttt attttagtca
    17221 tcatagttaa tacgccttgt gtattcctga cggcaaaaat aaggaatata aagaaatgat
    17281 tgctttggtt tcttacaaca catcattccc ttttcattta ataaagcggc atgatacaaa
    17341 gtgaacatga tttcgattta cattgccacc ataccgcttc agacaaccgg tacattactt
    17401 tgttataaga cgtgatatat aattcaaaac aacgtggttt
//
LOCUS       DAOFQD010000100        17438 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.100, whole genome shotgun sequence.
ACCESSION   DAOFQD010000100 DAOFQD010000000
VERSION     DAOFQD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.100"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            432..677
                     /locus_tag="QBF46_003433"
     CDS             432..677
                     /locus_tag="QBF46_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675574.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGWGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAVIRQIRLIT"
     gene            complement(694..1365)
                     /locus_tag="QBF46_003434"
     CDS             complement(694..1365)
                     /locus_tag="QBF46_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDQ6675575.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            1512..1787
                     /locus_tag="QBF46_003435"
     CDS             1512..1787
                     /locus_tag="QBF46_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ6675576.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(1885..3471)
                     /gene="prpR"
                     /locus_tag="QBF46_003436"
     CDS             complement(1885..3471)
                     /gene="prpR"
                     /locus_tag="QBF46_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDQ6675577.1"
                     /translation="MAYPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDALQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLRYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            3710..4600
                     /gene="prpB"
                     /locus_tag="QBF46_003437"
     CDS             3710..4600
                     /gene="prpB"
                     /locus_tag="QBF46_003437"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDQ6675578.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            4947..6116
                     /gene="prpC"
                     /locus_tag="QBF46_003438"
     CDS             4947..6116
                     /gene="prpC"
                     /locus_tag="QBF46_003438"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDQ6675579.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            6150..7601
                     /gene="prpD"
                     /locus_tag="QBF46_003439"
     CDS             6150..7601
                     /gene="prpD"
                     /locus_tag="QBF46_003439"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDQ6675580.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGSMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPSFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            7641..9527
                     /gene="prpE"
                     /locus_tag="QBF46_003440"
     CDS             7641..9527
                     /gene="prpE"
                     /locus_tag="QBF46_003440"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDQ6675581.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            9857..11116
                     /gene="codB"
                     /locus_tag="QBF46_003441"
     CDS             9857..11116
                     /gene="codB"
                     /locus_tag="QBF46_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDQ6675582.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            11106..12389
                     /gene="codA"
                     /locus_tag="QBF46_003442"
     CDS             11106..12389
                     /gene="codA"
                     /locus_tag="QBF46_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDQ6675583.1"
                     /translation="MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(12536..13435)
                     /gene="cynR"
                     /locus_tag="QBF46_003443"
     CDS             complement(12536..13435)
                     /gene="cynR"
                     /locus_tag="QBF46_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDQ6675584.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLRLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLNMALEECAVVGGNESR"
     gene            13544..14203
                     /gene="cynT"
                     /locus_tag="QBF46_003444"
     CDS             13544..14203
                     /gene="cynT"
                     /locus_tag="QBF46_003444"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDQ6675585.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLSNL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            14232..14702
                     /gene="cynS"
                     /locus_tag="QBF46_003445"
     CDS             14232..14702
                     /gene="cynS"
                     /locus_tag="QBF46_003445"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDQ6675586.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            14708..15889
                     /gene="cynX"
                     /locus_tag="QBF46_003446"
     CDS             14708..15889
                     /gene="cynX"
                     /locus_tag="QBF46_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDQ6675587.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHTLCVVGLMIITLRFAPVRFPQLWVKE
                     A"
     gene            complement(15992..16603)
                     /gene="lacA"
                     /locus_tag="QBF46_003447"
     CDS             complement(15992..16603)
                     /gene="lacA"
                     /locus_tag="QBF46_003447"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDQ6675588.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
     gene            complement(16669..>17438)
                     /gene="lacY"
                     /locus_tag="QBF46_003448"
     CDS             complement(16669..>17438)
                     /gene="lacY"
                     /locus_tag="QBF46_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDQ6675589.1"
                     /translation="TINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHS
                     AFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYV
                     TTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLH
                     MFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQG
                     AYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA"
BASE COUNT         4054 a         4546 c         4738 g         4100 t
ORIGIN      
        1 tccgctacac tctttagttc agaaagggtt ccacctgcaa gttgttattc caccagagag
       61 tgaatcgcaa tgcaggtaat catttcatct gtatatctgt atgccaactg gcattccatc
      121 tcttttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagtag
      181 tggtttcgca gccatgcggt gtataaaaaa tgatctcatg cagatgtttt gtgaatgtgt
      241 gggttgacat tcatatgaaa aaaaaatcat aattccatca tgtttgtgtg gggtcttttc
      301 tgtatcttac gcatcgcact caagcctgac agaaaatatg ctgtaaggct catatcaaaa
      361 accgccatta gctcatcagg aagagcagac gacaaccagg attgttgtat ggtacggggt
      421 tcgaggcctc gatggcggtc caacttttca aaactcttct gaatcagatc cccttattat
      481 catccctgca aagtggaaca ttgccattgt ttggctattc aggatggggc agaccaatga
      541 aaaaagccca cacaggggag agtgggctga aatgggaagc taaagactca agtaaactta
      601 tcggaaataa ggaccacgca ttacgggggc tatcatcgcc gatggccgtg attcggcaaa
      661 tcaggttgat tacgtagaga gtaaattatc tgctcaccgc tgcgtcaccc cttcgtaaat
      721 caggcgtagc gcgaatacac caataattgc accaataacc cgactggcaa cgcgttgcat
      781 acgcccataa gcacgacgca cagcgggcaa agaaaacgcc tgactaagaa aaactcgcca
      841 gataattgat gcgagcacaa tccccgccca ggccattaaa cgtgcccatg ttggtgtttc
      901 ggcatttaat gttactgaga aaatactgat aaaaaataaa acggtttgcg ggttagagag
      961 atcggtaatt aatccgcggc gaaaaaagac ataccagggg gcgctaatcg gttgttgtag
     1021 tgtgctcatt tgcggtgttg actggcggcg catgctgcac cacgcaaacc ataagagata
     1081 agcgccgccg acgattctga taagcgaaaa aatctcctca cactgcgtaa ttagcgttgc
     1141 aagaccaaac aaacccaacc cggaataaaa tgcatcgccc agcgccacgc ccagcccggt
     1201 cagcacccct gcgcgtcgac cggaagccag gctggtttgt actaccacaa agagattggc
     1261 tcccggatta aaaaaagtaa tcacgaacag tcctacggtc aggtaaacgg catgcaaagg
     1321 atccatagtg atttcatcca taaataagtg aactaactgc atcatatttc taccaaaaat
     1381 aatcgggtgc gagagagatc acaaagtgtc ttatttccgg ttactggcgt ttatgccctg
     1441 actgaactaa ttattaatca acccaataat gtcggtgggt gatagtgtga taacaactct
     1501 ggagccgtaa tatgaaaata atctctaaaa tgttagtcgg tgcgttagcg tttgccgtta
     1561 ccaatgtcta tgccgctgaa ttgatgacta aagcggaatt tgaaaaagtc gaatcgcaat
     1621 atgaaaaaat aggtgatatt tcaaccagca atgaaatgtc gaccgcagat gcgaaagaag
     1681 atttaatcaa aaaagccgat gaaaaagggg ctgatgtgtt ggtactgacc tccggtcaaa
     1741 ctgacaataa gatccacggc acggcggata tttataagaa gaagtaattc tgaatcctat
     1801 gtaaacatcc ccgatgcgta agtttatcgg tgatcatcta ttgaaattta tgccggataa
     1861 agcgttcgcg ctgcattcgg cagttcagct tttcagccgc cgccagaacg tcgtccggct
     1921 gatgcctaaa taattcgccg ctgctgtttt atcgccatta aatttctcca gtgcctgttg
     1981 tggtgtcagc aagcgtggag cgggaatttt cgccgactcg cgcgccagtt ccggcagtag
     2041 cagctgcaaa aactgcggcg ttaaatccgg cgtcggttcc acacttaaaa acagcgccag
     2101 tcgctccatc atattgcgca gttctcgaat attgcccggc cagtcgtagc gcagcagcac
     2161 agtttcactt gcctgtaacc cctggcgtaa tgcagcagaa aatggggcgg agagcgccgc
     2221 cagagacact ttcaaaaaac tttccgccag tggcagaata tctgccaccc gctcgcgcag
     2281 tggtggcaat tgcagacgca aaatactcag ccgataaaac agatcacggc gaaactgccc
     2341 ttgccgcata tcttcttcca gattgcagtg agtggcgcta atgacccgca catccaccgg
     2401 aacaggctga tgcccgccga cgcgagtaac ctctttttct tccagcaccc gtaacaggcg
     2461 agtctgcaaa ggtagcggca tttcgccaat ctcatccaaa aacagcgtgc caccgtgggc
     2521 gatttcgaac agcccggcac gacctccgcg tcgcgagccg gtaaatgccc cttcctcata
     2581 gccaaacagc tctgcttcca gcagcgattc ggcaatcgcc ccgcagttga cggcaacaaa
     2641 cggatgcgac tttttgccct gtagcgcatc gtggcgggca aagtattccc gatgaattgc
     2701 ctgggctgcc agttctttgc ccgtccccgt ttccccctca atcaacaccg ccgcactgga
     2761 gcgggcatat agcaaaatag tctgccgtac ctgttccatc tgtggtgatt gaccgagcat
     2821 atcgcccagc acgtaacgag tacgcagggc gttgcgggtg gcatcgtgag tgttatggcg
     2881 taacgacatg cgcgtcatat ccagcgcatc gctgaacgcc tggcgcacgg tggcggcgga
     2941 atagataaaa attccggtca ttccggcttc ttctgccaga tcggtaatca gccccgcgcc
     3001 gaccaccgct tcggtgccgt tagcttttag ctcgttaatc tgcccgcgtg cgtcttcttc
     3061 ggtaatgtag ctacgttgat cgaggcgcaa attaaaggtt ttttgaaacg ccaccagtgc
     3121 cggaatagtt tcctgataag tgacaacgcc gatagaagag gtgagctttc cggcttttgc
     3181 cagtgcctgt aacacatcgt agccgctcgg tttaatcaaa ataactggca ctgacaggcg
     3241 gcttttcagg tacgcgccgt tagagccagc agcgatgatg gcgtcacagc gttcgtttgc
     3301 cagtttcttg cggatgtagg tcaccgcttt ttcaaagcca agctgaatag gggtgatatt
     3361 cgccaggtga tcaaactcga ggctgatatc gcgaaacagc tcgaataagc gcgttacaga
     3421 taccgtccag ataaccggtt tgtcgtcatt aagccgtggt ggatatgcca tagcgcaccg
     3481 caaagttaag aaaccgaata ttgggtttag ccttgtttca taattgttgc aatgaaacgc
     3541 ggtgaaacat tgcctgaaac gttaactgaa acgcatattt gcggattact tcatgacttt
     3601 atttataaca aattgaaatt aaacatttaa ttttattcag tcaattgtgg cacacccctt
     3661 gctttgtctt tattaacgca aataacaagt tgataacaaa ggatgggcta tgtctctaca
     3721 ctctccaggt aaagcgtttc gcgctgcact gactaaagaa aatccattgc agattgttgg
     3781 caccattaac gccaaccatg cgctgttggc gcagcgtgcc ggatatcagg caatttatct
     3841 ctccggcggt ggcgtggcgg cagggtcgct ggggttgccc gatctcggta tttctactct
     3901 tgatgacgtg ctgacagata ttcgccgtat caccgacgtt tgttcgctgc cgctgctggt
     3961 ggatgcggat atcggttttg gttcttctgc ttttaacgtg gcgcgtacgg tgaaatcgat
     4021 gattaaagcc ggtgcggcag gattgcatat tgaagatcag gttggtgcga aacgctgcgg
     4081 tcatcgtccg aataaagcga tcgtctcgaa agaggagatg gtggatcgga tccgcgcggc
     4141 ggtggatgcg aaaaccgatc ctgattttgt gatcatggcg cgcaccgatg cgctggcggt
     4201 agaggggctg gatgcggcga tcgagcgtgc gcaggcctat gttgaagcgg gtgccgagat
     4261 gttgttcccg gaggcgatta ccgaactcgc catgtaccgc cagtttgccg atgcggtgca
     4321 ggtgccgatc ctcgccaata ttaccgaatt tggtgccacg ccgctgttta ctaccgatga
     4381 attacgcagc gcccatgtcg caatggcgct gtacccactt tcagcgttcc gcgccatgaa
     4441 ccgcgccgct gagcatgtct acaacgtcct gcgtcaggaa ggcacgcaga aaagcgtcat
     4501 cgataccatg caaacccgca acgagttgta cgaaagcatc aactactacc agtacgaaga
     4561 gaagctcgac gacctgtttg cccgtagcca ggtgaaataa aaaacgcccg ttggttgtat
     4621 tcgacaaccg atgcctgatg cgccgctgac gcgacttatc aggcctacgg ggtgtactga
     4681 actgtaggtc ggataagacg catggcatcg catccgacaa ccgatgcctg atgcgtcgct
     4741 gacgcgactt atcaggccta cggggtgaac tgaactgtag gtcggataag acgcatggcg
     4801 tcgcatccga caaccgatgc ctgatgcgcc gctgacgcgg cttatcaggc ctacggggtg
     4861 tactgaactg taggtcggat aagacgcatg gcgtcgcatc cgacaacaat ctcgacccta
     4921 caaatgataa caatgacgag gataatatga gcgacacaac gatcctgcaa aacagtaccc
     4981 atgtcattaa accgaaaaaa tctgtggcac tttctggcgt tccggcgggc aatacggcgc
     5041 tctgcaccgt gggtaaaagt ggcaatgact tgcattaccg tggttacgat attcttgatc
     5101 tggcggaaca ttgcgaattt gaagaagtgg cgcatctgct gatccacggc aaactgccga
     5161 cccgtgacga actcgccgcc tacaaaacga aactgaaagc cctgcgcggt ttaccggcta
     5221 acgtgcgtac tgtgctggaa gccttaccgg cggcgtcgca cccgatggat gttatgcgca
     5281 ccggcgtttc cgcgctcggc tgcacgctgc cagaaaaaga ggggcatacc gtctccggcg
     5341 cgcgggatat tgccgacaaa ctgttggcgt cgctgagctc gattcttctc tactggtatc
     5401 actacagcca caacggcgaa cgcatccagc cggaaaccga tgacgactca ataggcggtc
     5461 acttcctgca tctgctgcac ggcgaaaagc catcgcaaag ctgggaaaag gcgatgcata
     5521 tttcgctagt gctgtacgcc gaacacgagt ttaacgcctc cacctttacc agtcgggtga
     5581 ttgcgggcac tggctctgat atgtattccg cgattattgg cgcgattggc gcactgcgcg
     5641 ggccgaagca cggcggggcg aatgaagtgt cgctggagat ccagcaacgc tacgaaacgc
     5701 cagacgaagc cgaagccgat atccgcaagc gggtggaaaa caaagaagtg gtcattggtt
     5761 ttggtcatcc ggtttatacc atcgccgacc cgcgccacca ggtgattaaa cgtgtggcga
     5821 agcagctctc gcaggaaggc ggctcgctga agatgtacaa catcgccgat cgcctggaaa
     5881 cggtgatgtg ggagagcaaa aagatgttcc ccaatctcga ctggttctcc gctgtttcct
     5941 acaacatgat gggcgttccc accgagatgt tcacaccact gtttgttatc gcccgcgtca
     6001 ccggctgggc ggcgcacatt atcgaacaac gtcaggacaa caaaattatc cgtccttccg
     6061 ccaattatgt tggaccggaa gaccgccagt ttgtcgcgct ggataagcgc cagtaaacct
     6121 ctacgaataa caataaggaa acgtacccaa tgtcagctca aatcaacaac atccgcccgg
     6181 aatttgatcg tgaaatcgtt gatatcgtcg attacgtcat gaactacgaa atcagctcta
     6241 aagtggccta cgacaccgca cattactgcc tgctcgacac gctcggctgc ggtcttgaag
     6301 ctctcgaata cccagcctgt aaaaaactgc tggggccaat tgtccccggc accgtcgtac
     6361 ctaacggcgt gcgcgtcccc ggaactcagt tccagctcga ccccgtccag gcggcattta
     6421 acatcggctc gatgatccgt tggctcgatt tcaacgatac ctggctggcg gcggagtggg
     6481 ggcatccttc cgacaacctc ggcggcattc tggcaacggc agactggctt tcgcgcaacg
     6541 cggtcgccag cggcaaagcg ccgttgacca tgaaacaggt gctgaccgga atgattaaag
     6601 cccatgaaat tcagggctgc atcgcgctgg aaaactcctt taaccgcgtc ggcctcgacc
     6661 acgttctgtt agtgaaagtg gcttccaccg ccgtggtcgc cgaaatgctt ggcctgaccc
     6721 gcgaggaaat tctcaacgct gtttcgctgg cgtgggtgga cggtcagtcg ctgcgcacct
     6781 atcgccatgc gccgaacacc ggcacgcgta aatcctgggc ggcgggcgat gccacttccc
     6841 gcgcggtacg tctggcactg atggcgaaaa cgggcgaaat gggttacccg tcagccctga
     6901 ccgcgcctgt gtggggcttc tacgacgtct cctttaaagg tgagtcgttc cgcttccagc
     6961 gtccgtacgg ttcttacgtg atggaaaatg tgctgttcaa aatctccttc ccggcggagt
     7021 tccactccca gacggcagtt gaagcagcga tgacgctcta tgaacagatg caggcagcag
     7081 gcaaaacggc ggcggatatc gaaaaagtga ccattcgcac ccacgaagcc tgtattcgca
     7141 tcatcgacaa aaaggggccg ctcaataacc cggcagaccg cgaccactgc attcagtaca
     7201 tggtggcgat cccactgctg ttcgggcgct taacggcggc agattacgag gacaacgttg
     7261 cgcaagataa acgcatcgac gccctgcgcg agaagatcaa ttgctttgaa gatccgtcat
     7321 ttaccgctga ctaccacgac ccggaaaaac gcgctattgc caatgccata acccttgagt
     7381 tcaccgacgg cacgcgcttt gaagaagtgg tggtggagta ccccattggt catgctcgcc
     7441 gccgtcagga tggcattccg aagctggtgg ataaattcaa aatcaatctc gcgcgccagt
     7501 tcccgactcg ccagcagcag cgcattctgg aggtttctct cgacagaact cgcctggaac
     7561 agatgccggt caatgagtat ctcgacctgt acgtcattta agcaaacagc ggtaaggcgt
     7621 aagttcaaca ggagagcatt atgtctttta gcgaatttta tcagcgttcg attaacgaac
     7681 cggagcagtt ctgggccgag caggcccggc gtattgactg gcagacgccc tttacgcaaa
     7741 cgctcgatca cagcaatccg ccgtttgccc gttggttttg tgaaggccga accaacttgt
     7801 gtcacaacgc tatcgaccgc tggctggaga aacagccaga ggcgctggcg ctgattgccg
     7861 tctcttcgga aacagaagaa gagcgcacct ttacctttcg tcagctgcat gacgaagtga
     7921 atgcggtggc gtcaatgctg cgctcgctgg gcgtgcagcg tggcgatcgg gtgctggtgt
     7981 atatgccgat gattgccgaa gcgcatatta ctctgctggc ctgcgcgcgc attggcgcta
     8041 ttcactcggt ggtgtttggt ggatttgcct cgcacagcgt ggcggcgcga attgatgatg
     8101 ctaaaccggt gctgattgtc tcggcagatg ccggagcgcg cggtggtaaa atcattccct
     8161 ataaaaaatt gctcgacgat gcgataagtc aggcgcagca tcagccgcgc cacgttttgc
     8221 tggtggatcg cgggctggcg aaaatggcgc gcgttagcgg gcgggatgtc gatttcgcgt
     8281 cgttgcgcca tcaacacatc ggcgcgcggg tgccggtggc gtggctggaa tccaacgaaa
     8341 cctcctgcat tctctacacc tccggcacga ccggcaaacc taaaggcgtg cagcgtgacg
     8401 ttggcggata tgcggtggcg ctggcgacct cgatggacac catttttggc ggcaaagcgg
     8461 gcggcgtgtt cttctgtgct tcggatatcg gctgggtggt ggggcattcg tatatcgtct
     8521 acgcgccgct gctggcgggg atggcgacta tcgtttacga aggattgccg acctggccgg
     8581 actgcggcgt gtggtggaaa atcgtcgaga aatatcaggt tagccggatg ttctcagcgc
     8641 cgaccgccat tcgcgtgctg aaaaaattcc ctaccgctga aattcgtaaa cacgatctct
     8701 cgtcgctgga agtgctctat ctggctggag aaccgctgga cgagccgacc gccagttggg
     8761 tgagcaatac gctggatgtg ccggtcatcg acaactactg gcagaccgaa tccggctggc
     8821 cgattatggc gattgctcgc ggtctggacg acaggccgac gcgtctggga agccccggcg
     8881 tgccgatgta tggctataac gtgcagttgc ttaatgaagt caccggcgaa ccgtgtggcg
     8941 tcaacgagaa agggatgctg gtggtggaag ggccgctgcc gccggggtgt attcagacca
     9001 tctggggcga cgacggccgc tttgtgaaga cttactggtc gctgttttcc cgcccggtgt
     9061 acgccacctt tgactggggc atccgtgacg ctgacggtta tcactttatt ctcgggcgca
     9121 ctgacgatgt aattaacgtt gccgggcatc ggctggggac gcgcgagatt gaagagagta
     9181 tctccagcca tccgggcgtt gccgaagtgg cggtggttgg ggtgaaagat gcgctgaaag
     9241 ggcaggtggc ggtggcgttt gtcattccga aagagagcga cagtctggaa gatcgtgatg
     9301 tggcgcattc gcaagagaag gcgattatgg cgctggtgga cagccagatt ggcaactttg
     9361 gccgcccggc gcacgtctgg tttgtctcgc aattgccaaa aacgcgatcc ggaaaaatgc
     9421 tgcgccgcac gatccaggcg atttgcgaag gacgcgatcc tggagatctg acgaccattg
     9481 atgatcctgc gtcgttggat cagatccgcc aggcgatgga agagtaggtt attgtcggat
     9541 gcgtcgcgcg gtgcatccgg cactgtgtgc cgatgcctga tgcgacgctg acgcgtctta
     9601 tcatgcctac ggacctgaac cgtaggtcgg ataaggcgct cgcgtcgcat ccgacaccat
     9661 gctcagatgc ctgatgcgac gctgacgcgt cttatcaggc ctacccactg tttttacacc
     9721 gataattttt cccccacctt tttgcactca ttcatataaa aaatatattt ccccacgaaa
     9781 acgattgctt tttatcttca gatgaataga atgcggcgga ttttttgggt ttcaaacagc
     9841 aaaaaggggg aatttcgtgt cgcaagataa caactttagc caggggccag tcccgcagtc
     9901 ggcgcggaaa ggggtattgg cattgacgtt cgtcatgctg ggattaacct tcttttccgc
     9961 cagtatgtgg accggcggca ctctcggaac cggtcttagc tatcatgatt tcttcctcgc
    10021 agttctcatc ggtaatcttc tcctcggtat ttacacttca tttctcggtt acattggcgc
    10081 aaaaaccggc ctgaccactc atcttcttgc tcgcttctcg tttggtgtta aaggctcatg
    10141 gctgccttca ctgctactgg gcggaactca ggttggctgg tttggcgtcg gtgtggcgat
    10201 gtttgccatt ccggtgggta aggcaaccgg gctggatatt aatttgctga ttgccgtttc
    10261 cggtttactg atgaccgtca ccgtcttttt tggcatttcg gcgctgacgg ttctttcggt
    10321 gattgcggtt ccggctatcg cctgcctggg cggttattcc gtgtggctgg ctgttaacgg
    10381 catgggcggc ctggacgcat taaaagcggt cgttcccgca caaccgttag atttcaatgt
    10441 cgcgctggcg ctggttgtgg ggtcatttat cagtgcgggt acgctcaccg ctgactttgt
    10501 ccggtttggt cgtaatgcca aactggcggt gctggtggcg atggtggcct ttttcctcgg
    10561 caactcgttg atgtttattt tcggtgcagc gggcgctgcg gcactgggca tggcggatat
    10621 ctctgatgtg atgattgctc agggcctgct gctgcctgcg attgtggtgc tggggctgaa
    10681 tatctggacc accaacgata acgcactcta tgcgtcgggt ttaggtttcg ccaacattac
    10741 cgggatgtcg agcaaaaccc tttcggtaat caacggtatt atcggtacgg tctgcgcatt
    10801 atggctgtat aacaattttg tcggctggtt gaccttcctt tcggcagcta ttcctccagt
    10861 gggtggcgtg atcatcgccg actatctgat gaaccgtcgc cgctatgagc actttgcgac
    10921 cacgcgtatg atgagtgtca attgggtggc gattctggcg gtcgccttgg ggattgctgc
    10981 aggccactgg ttaccgggaa ttgttccggt caacgcggta ttaggtggcg cgctgagcta
    11041 tctgatcctt aacccgattt tgaatcgtaa aacgacagca gcaatgacgc atgtggaggc
    11101 taacagtgtc gaataacgct ttacaaacaa ttattaacgc ccggttacca ggcgaagagg
    11161 ggctgtggca gattcatctg caggacggaa aaatcagcgc cattgatgcg caatccggcg
    11221 tgatgcccat aactgaaaac agcctggatg ccgaacaagg tttagttata ccgccgtttg
    11281 tggagccgca tattcacctg gacaccacgc aaaccgccgg acaaccgaac tggaatcagt
    11341 ccggcacgct gtttgaaggc attgaacgct gggccgagcg caaagcgtta ttaacccatg
    11401 acgatgtgaa acaacgcgca tggcaaacgc tgaaatggca gattgccaac ggcattcagc
    11461 atgtgcgtac ccatgtcgat gtttcggatg caacgctaac tgcgctgaaa gcaatgctgg
    11521 aagtgaagca ggaagtcgcg ccgtggattg atctgcaaat cgtcgccttc ccgcaggaag
    11581 ggattttgtc gtatcccaac ggtgaagcgt tgctggaaga ggcgttacgc ttaggggcag
    11641 atgtagtggg ggcgattccg cattttgaat ttacccgtga atacggcgtg gagtcgctgc
    11701 ataaaacctt cgccctggcg caaaaatacg accgtctcat cgacgttcac tgtgatgaga
    11761 tcgatgacga gcagtcgcgc tttgtcgaaa ccgttgctgc cctggcgcac cgtgaaggca
    11821 tgggcgcgcg agtcaccgcc agccacacca cggcaatgca ctcctataac ggggcgtata
    11881 cctcacgcct gttccgcttg ctgaaaatgt ccggtattaa ctttgtcgcc aacccgctgg
    11941 tcaatattca tctgcaagga cgtttcgata cgtatccaaa acgtcgcggc atcacgcgcg
    12001 ttaaagagat gctggagtcc ggcattaacg tctgctttgg tcacgatgat gtcttcgatc
    12061 cgtggtatcc gctgggaacg gcgaatatgc tgcaagtgct gcatatgggg ctgcatgttt
    12121 gccagttgat gggctacggg cagattaacg atggcctgaa tttaatcacc caccacagcg
    12181 caaggacgtt gaatttgcag gattacggca ttgccgccgg aaacagcgcc aacctgatta
    12241 tcctgccggc tgaaaatggg tttgatgcgc tgcgccgtca ggttccggta cgttattcgg
    12301 tacgtggcgg caaggtgatt gccagcacac aaccggcaca aaccaccgta tatctggagc
    12361 agccagaagc catcgattac aaacggtgat cggctgggct gtgtgttgac aaagcgagcg
    12421 tagtttatgc cggatgcggc gtgaacgcct tatccggcct acgtagagca ctgaactcgt
    12481 aggcctgata agcgtagcgc atcaggcaat tccagccgca gacctgtgtc agcggctacc
    12541 gtgattcatt cccgccaaca accgcgcatt cctccaacgc catgttcaaa aatgccttcg
    12601 cggcggctgt ctgccagcta tttttccgcc gcaacaaaac cgccgttctc tccagtagtg
    12661 gcggggcaag agaaatcgct ttaagcccgt catgttgtgt ggcaatcgct gctggtaaca
    12721 atgtggaaag ggaagtgcgg cgaatcagct ccagaaccgc gctaattgag ttcgcctcaa
    12781 tgaccacctg tggatgcagc cccgctttct cgcagtagtg gtcaatttgc tctctggtgg
    12841 caaattccgc gctgagcagt accagttttt catcatgcaa gcgactcaac gccacctgtt
    12901 cacaggcagc cagcggatga tgttgcgcca cgactaacgc taaactttct gtcagtaaag
    12961 gaattgcctc cagctccggc gaatgcacag gcgcgaaggc aatcccaacg tccaactcgt
    13021 cgcggcaaag cagatcttcg attttctcct gcgacatttc ctgtagccgg agtgtgatgc
    13081 cggggtagcg cgcatagaaa tccgccatta aagggccgat aaagtagctt gtaaaggtgg
    13141 gagtgacggc gatacgcagc gatcctcgcg tcagatcggc aacatcatga atcgcccgtt
    13201 tacccgcccc cagttcctgt aacgcccggc tggcgtactg tcgccagact tctcctgcat
    13261 cagtgagacg aatcgttcgc ccgctacggt caaacagcgg cacgcctaaa ctctcctcta
    13321 actggcgaat ctgctgggaa agcgcaggct gagagacgtg caacgcactg gcggcacggg
    13381 tgaagctgcc atgttcagcc acggcaagaa aatagttgat atgtcgagag agcatttgcc
    13441 acctataagt aaatccaata gaactcatca taaatgagac ttttacctta tgacaatcgg
    13501 cgagtagtct gcctctcatt ccagagacag acagaggtta acggtgaaag agattattga
    13561 tggattcctt aaattccagc gcgaggcatt tccgaagcgg gaagccttgt ttaaacagct
    13621 ggcgacacag caaagcccgc gcacactttt tatctcctgc tccgacagcc gtctggtccc
    13681 tgagctggtg acgcaacgtg agcctggcga tctgttcgtt attcgcaacg cgggcaatat
    13741 cgtcccttcc tacgggccgg aacccggtgg cgtttctgct tcggtggagt atgccgtcgc
    13801 tgcgcttcgg gtatctgaca ttgtgatttg cggtcattcc aactgtggcg cgatgaccgc
    13861 cattgccagc tgtcagtgca tggaccatat gcctgccgtc tcccactggc tgcgttatgc
    13921 cgattcagcc cgcgtcgtta atgaggcgcg cccgcatccc gatttaccgt caaaagctgc
    13981 tgcgatggta cgtgaaaacg tcattgctca gttgtctaat ttgcaaactc atccatcggt
    14041 gcgcctggcg ctcgaagagg ggcggatcgc cctgcacggc tgggtctacg acattgaaag
    14101 cggcagcatc gcagcttttg acggcgcaac ccgccagttt gtgccactgg ccgctaatcc
    14161 tcgcgtttgt gccataccgc tacgccaacc gaccgcagcg taacttttta taatttatca
    14221 ggagatccac catgattcag tcgcaaatta accgcaatat tcgtcttgat cttgccgatg
    14281 ccattttgct cagcaaagct aaaaaagatc tctcatttgc cgagattgcc gacggcaccg
    14341 ggctggcaga agcctttgtg accgcggctt tgctgggtca gcaggcgctt cctgccgacg
    14401 ccgcccgcct ggtcggggcg aagctggatc tcgacgaaga cgccattcta ctgttgcaga
    14461 tgattccact gcgtggctgc attgatgacc gtattccaac cgacccaacg atgtatcgtt
    14521 tctatgaaat gttgcaggtg tacggcacaa ccctgaaagc gttggttcat gagaagtttg
    14581 gcgatggcat tatcagcgcg attaacttca aactcgacgt taagaaagtg gcggacccgg
    14641 aaggtggcga acgtgcggtc atcaccttag atggtaaata tctgccgacc aaaccgttct
    14701 gacagccatg cgcaaccatc aaaagacgtt cacgatgctg ctggttctgg tgctgattgg
    14761 tcttaatatg cgaccactgc tcacctccgt cggtccactg ctaccgcaat tgcgccaggc
    14821 gagcggaatg agctttagcg tggctgccct gttgaccgct ctgccggtgg tcaccatggg
    14881 cgggctggcg ctggccggaa gctggcttca tcagcatgtc agcgaacgtc gcagcgtcgc
    14941 catcagtctg ttgctgattg ccgtcggtgc attgatgcgt gagctttacc cccaaagtgt
    15001 gctgctgctt agcagcgcac tgcttggtgg ggtggggatc ggtatcattc aggcggtgat
    15061 gccttcagtg attaaacggc ggtttcagca acgcacgcca ctggtgatgg ggctgtggtc
    15121 tgcggctctg atgggcggcg gtggacttgg tgccgccata acgccctggt tagttcaaca
    15181 tagcgaaacc tggtatcaaa cactcgcctg gtgggcgctg cctgccgttg ttgcgctctt
    15241 tgcctggtgg tggcaaagcg cccgcgaggt cgcctcttcc cacaagacaa caaccactcc
    15301 ggttcgcgtg gtattcactc cccgcgcgtg gacgctgggt gtttacttcg gtctgattaa
    15361 cggcggttac gccagcctga ttgcctggtt acccgctttc tatattgaga ttggtgccag
    15421 cgcgcagtac agcggttcct tactggcatt gatgacgctt gggcaagccg caggagcttt
    15481 gctgatgcct gctatggctc gccatcagga tcggcgcaaa ctgttaatgc tggcgctggt
    15541 gttacaactg gtggggttct gcggctttat ctggttgccg ctgcaattgc cggtattgtg
    15601 ggcgatggtg tgtgggttag gtctgggcgg cgcgtttccg ctctgtttgc tgctggcgct
    15661 cgatcactct gtgcaaccgg ctattgctgg caagctggtg gcgtttatgc agggaatcgg
    15721 ttttatcatc gccgggcttg ccccgtggtt ttctggcgtg ctgcgtagta tcagcggcaa
    15781 ttacctgatg gactgggcat ttcatacgct gtgcgtcgtt gggctgatga tcataaccct
    15841 gcgttttgca ccagtacgtt ttccgcagct gtgggtcaaa gaggcatgat gcgacgcttg
    15901 ttcctgcgct ttgttcatgc cggatgcggc taatgtagat cgctgaactt gtaggcctga
    15961 taagcgcagc gtatcaggca atttttataa tttaaactga cgattcaact ttataatctt
    16021 tgaaataata gtgcttatcc cggtcgttta tttcgcgaat aacccgacaa ggaacgccag
    16081 ccgccacgac gtttggtgga atgtcttttg tgacgatact acccgcgcca ataacagaat
    16141 tatccccgat ggtgacgcct ggattaataa ccacatgact tccgatccag acgttattgc
    16201 caatcgttat cggaaaagag tacatctcgc cgttttttct caattcatgg tgtacagggt
    16261 gtcccgtaac ggaaagagta acgttgggtg caatcagtac gttatcaccg attgttaccg
    16321 tgtagtcatc gacaatggtt aaattgaaat ttgcataaaa attgcggcct atatggatgt
    16381 tggaaccgta agagaaatag acaggcggtt ctacccaggc gttttcccct accgtggcaa
    16441 acatttcttt aatcaggctt tctctttttt caacttctga tggatgcgag tgattaaact
    16501 catacattaa cgttttccca cgaagtcttt tttccggtaa gccttcgcac atatcggtaa
    16561 atagcttgcc tgctcttatt ctttcggtca ttggcatgtt caatgcgatc actccgttat
    16621 gatatgttgg tcggataagg cgctcgcgcc gcatccgaca ttgattgctt aagcgacttc
    16681 attcacctga cgacgcagca gggaaagcgg gccggggccg ctaagcgtga acacggaaat
    16741 taaggtgaag cccagcgcca ccagacccag caccagataa gcgccctgga aaccgatgct
    16801 ttcatacata ttgcccgcca gtacagacat aaaaatcatc gccagttgct taaagaagca
    16861 gaaacagacc agataaatcg tcgctgaaaa acgcacttca aactggctgg taatatattt
    16921 aaagcagccc accagcagga acggtacttc aaacatatgc agcgttttca gaataaccac
    16981 ttccagcgct gaggtggcga acgatgagcc aataatacgt acagacataa tagtgccagc
    17041 cagcagcagg gcgtttttcc caccgatgcg attaatgatc agtggcgcaa agaacataat
    17101 cgaggcgtta agtaattcgc ccattgtcgt tacgtagcca aatacccgcg taccctgttc
    17161 accggtagca aagaacgaag taaagaaatt agcaaactgt tggtcaaaaa catcgtaggt
    17221 gcaggaaaca ccaataacat acagtgacaa aaaccacagt tttggctgtc tgaacagttc
    17281 cagtgccagc ttaaggctaa atgccgaatg gttggcacct accgcattgg caaccgtggc
    17341 agaagagggc gcatccgttt tggcgaaaaa gagtaaaacg gcgaggatga gtgcacagcc
    17401 agagcccagc cagaaaacaa actgattatt gatggtga
//
LOCUS       DAOFQD010000101        17361 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.101, whole genome shotgun sequence.
ACCESSION   DAOFQD010000101 DAOFQD010000000
VERSION     DAOFQD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.101"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(450..656)
                     /locus_tag="QBF46_003449"
     CDS             complement(450..656)
                     /locus_tag="QBF46_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDQ6675590.1"
                     /translation="MNTTNQSLIRLPEVLKRTGFGKAWIYRLISEGRFPAPVKIGVRA
                     VAFVESEVDEWIQSVIETSRIKVA"
     gene            complement(823..1632)
                     /locus_tag="QBF46_003450"
     CDS             complement(823..1632)
                     /locus_tag="QBF46_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675591.1"
                     /translation="MKVVSPYEELKSWFSLENYEQLKSLTIKELHTELAFREAIFDSV
                     NFGWEDDNQNLRSILEGNPILSRQDNNHGHFGKGQRHVAQVSFGDIKKLENKLIMFSM
                     DFFEENGDFKKELKKKPITKTMRDFFLNQNSSKMYVSFDLGMSSDEEIITALQTMLPD
                     LRKEYEIDPVKTEKIGLAKIRKLVDYNIIPMMDLLIWSKFKKVKISNMVLSRVLYPDF
                     TQEIRGEDHIKDTDRPVAEKSLNGETTRSLEYFISKNSHLLNIPISEFGTF"
     gene            complement(1744..2994)
                     /locus_tag="QBF46_003451"
     CDS             complement(1744..2994)
                     /locus_tag="QBF46_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6675592.1"
                     /translation="MALTDIKVRTTKPSDKPFKLTDGQGMHLLINPNGSKYWRLQYRF
                     DGKQKVLALGVYPMVSLGEARRKRDEAKKLVSDGIDPSEKKKADKIEQSEALTFESVA
                     RDWHKACKRKWSDSHSERVLKSMEDGLFTAIGKRKISELNTRDLLAPIKAVEASGRLE
                     VASRLQQRTTAVMRYAVQNGLIDYNPAQEMAGAIAVAKRTHRPALPFERFSELLERID
                     NFKGRKLTKLAVKLTLLIFIRSSELRFARWSEIDFENAMWTIPSEREPLPGVKHSHRG
                     SKMRTPHLVPLSCQALAVLTEIKEISGDHELVFIGDHDFRKPMSENTVNKALRSMGYD
                     TTVEVCGHGFRAMACSALIESGLWSKDAVERQMSHQERNSVRAAYIHKAEHLDERKLM
                     LQWWADYLDILRIRQVSPFDFKKT"
     gene            complement(3184..3268)
                     /locus_tag="QBF46_003452"
     tRNA            complement(3184..3268)
                     /locus_tag="QBF46_003452"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3232..3234),aa:Leu,seq:caa)
     gene            3464..4483
                     /gene="ahr"
                     /locus_tag="QBF46_003453"
     CDS             3464..4483
                     /gene="ahr"
                     /locus_tag="QBF46_003453"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDQ6675593.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            4613..6115
                     /locus_tag="QBF46_003454"
     CDS             4613..6115
                     /locus_tag="QBF46_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDQ6675594.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLDIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMHPTPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(6276..7358)
                     /gene="lptG"
                     /locus_tag="QBF46_003455"
     CDS             complement(6276..7358)
                     /gene="lptG"
                     /locus_tag="QBF46_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDQ6675595.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7358..8458)
                     /gene="lptF"
                     /locus_tag="QBF46_003456"
     CDS             complement(7358..8458)
                     /gene="lptF"
                     /locus_tag="QBF46_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDQ6675596.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            8725..10236
                     /gene="pepA"
                     /locus_tag="QBF46_003457"
     CDS             8725..10236
                     /gene="pepA"
                     /locus_tag="QBF46_003457"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDQ6675597.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            10370..10813
                     /gene="holC"
                     /locus_tag="QBF46_003458"
     CDS             10370..10813
                     /gene="holC"
                     /locus_tag="QBF46_003458"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDQ6675598.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10813..13668
                     /gene="valS"
                     /locus_tag="QBF46_003459"
     CDS             10813..13668
                     /gene="valS"
                     /locus_tag="QBF46_003459"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDQ6675599.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEDQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(13724..14920)
                     /locus_tag="QBF46_003460"
     CDS             complement(13724..14920)
                     /locus_tag="QBF46_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDQ6675600.1"
                     /translation="MAQVINEMDVPSHSFVFRGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGAV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            15113..15616
                     /locus_tag="QBF46_003461"
     CDS             15113..15616
                     /locus_tag="QBF46_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6675601.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(15662..16078)
                     /gene="rraB"
                     /locus_tag="QBF46_003462"
     CDS             complement(15662..16078)
                     /gene="rraB"
                     /locus_tag="QBF46_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDQ6675602.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            16240..17244
                     /gene="argF"
                     /locus_tag="QBF46_003463"
     CDS             16240..17244
                     /gene="argF"
                     /locus_tag="QBF46_003463"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDQ6675603.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRTLAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT         4554 a         4260 c         4275 g         4272 t
ORIGIN      
        1 atgtacacct aatacatcga aaactatctc actgccttgc taataaacga tcataaaaat
       61 aatgtaagtt attctgtaaa gttttaagca aaaatatgca gccaaacttt acattttcta
      121 aatatttctt aatccaagag atataggatt atccgcttcg ccaagagttt catttcatca
      181 gggcatttaa ttagcgagaa ccatgtctcg taaacatata tctgtttgga agcattaagc
      241 tcagagatcg agtctaaagg ttctctcaaa tttcctacaa ggtactgact gagccagtaa
      301 attatttctt tattaaactc gttattgggt aagtcataaa tcacttctgg gtcaaagatc
      361 ttgtaaaaca gatctccgca taaattgcac atatggttat cttcactcac aaattcttcg
      421 atattcataa aaatctctcc caggtttaat taagcaactt taattctgct tgtttcgata
      481 actgattgaa tccactcgtc aacttcactc tcgacaaaag caacggcacg aactccgatc
      541 ttcactggtg ccggaaaccg accttcacta atcagacgat agatccaggc cttgccgaaa
      601 ccagttcgtt tcaagacttc tggtaaacga attaagcttt gattggttgt attcatttgg
      661 ctgtctcctc cgcttccgtt aaggagatat tagccacaca gatttgtatc tatgaaggga
      721 atgctgataa ctgaaaagtt agatgaggga agaatgaaga gagactccgg ggaagaaaaa
      781 tattttcgcc ctccccttga caattaacac aatgatttac aactagaaag ttccgaattc
      841 tgaaatcggt atgttgagga gatggctgtt tttgctgata aaatattcca gactacgggt
      901 tgtttctcca tttaatgact tctctgctac gggcctgtct gtatctttaa tgtgatcttc
      961 gcccctaatc tcctgagtga aatcaggata caagacacga gacaagacca tatttgagat
     1021 ttttactttt ttgaattttg accaaatcaa taggtccatc attggaatga tattgtaatc
     1081 gacaagtttt cttatttttg ccagtccaat tttttctgtt tttactgggt caatttcata
     1141 ttccttccta agatctggta gcatcgtttg taaggctgtt ataatttctt catcgctgga
     1201 cattcctaaa tcaaatgaaa catacatctt gctgctattt tggtttagaa aaaagtctct
     1261 catagttttt gttattggtt tctttttcaa ctcttttttg aaatcgccat tttcttcaaa
     1321 aaaatccatg ctaaacatta ttaacttgtt ctctaatttt tttatatcac caaagctaac
     1381 ctgcgcaaca tgtctttgac ccttgccaaa atgaccatga ttattatctt gtctgcttaa
     1441 aataggatta ccttcaagga tagaccttaa attttgatta tcatcttccc atccaaaatt
     1501 tacactgtcg aaaatcgctt ctctaaaggc aagctcagta tgcagttctt tgattgtaag
     1561 tgattttaac tgctcatagt tttcgagact aaaccagctt tttaattctt cataagggct
     1621 tactactttc attaatccct ccactgtatt caaagcttga ataagttaac actgcttatt
     1681 tcaattaatt ctcccttaac aatattgata agggagaacg attaattaac tacttattca
     1741 tagttaagtc tttttaaaat caaatggtga aacttgtcgt attctaagga tgtcaaggta
     1801 atcggcccac cactgcaaca taagctttct ctcatcaagg tgctctgctt tatgtatgta
     1861 ggcagcacga actgagttcc gctcctgatg gctcatttgc cgttcaacag catctttcga
     1921 ccacaaaccg gattcaatca aggcactaca agccatagcc ctgaatccat gtccacagac
     1981 ctccacggtt gtgtcatagc ccatagacct caaagccttg tttaccgtat tttcactcat
     2041 aggctttctg aaatcatggt cgccaatgaa cacgagttca tgatcaccgc tgatctcttt
     2101 aatttccgtc aacacagcca atgcctgaca actcaaagga actaggtgtg gagttcgcat
     2161 tttagaacca cggtgcgaat gcttcactcc tggtaatggt tctcgttcag agggaatagt
     2221 ccacatagca ttctcgaaat cgatttccga ccatcttgca aatctgagtt cactggaacg
     2281 aatgaaaatg agcaatgtta gctttacggc taacttcgtg agtttcctac ctttgaagtt
     2341 atcaattcgt tctaagagtt ctgagaatcg ttcgaatggc aaagctggcc tgtgggttct
     2401 tttggctacg gcaatagctc ctgccatctc ctgagcaggg ttgtaatcta tcagtccatt
     2461 ctggaccgca tatcgcatta ctgctgttgt tctttgctgt aggcgggaag caacttccaa
     2521 tcgcccggaa gcttcaacag ccttgatagg tgcaagaaga tctctggtat taagctcgga
     2581 tatttttctt ttaccaattg cagtaaagag accatcttcc atgcttttta gaactctctc
     2641 gctgtgggaa tctgaccatt ttcttttaca agccttatgc cagtccctgg caacagattc
     2701 aaaagtaaga gcttcgctct gctcgatttt gtcggctttt ttcttctcag aagggtcgat
     2761 accatcagat accaactttt tggcttcatc tcgcttcctg cgggcctcac caagagagac
     2821 cattggataa acacccaatg ctaaaacttt ctgtttgccg tcgaaacggt actgtaatcg
     2881 ccagtacttc gagccgttag gattgatcag caggtgcata ccctgcccat ctgtgagttt
     2941 gaagggtttg tcggagggct tggtagtcct aactttgata tcggtgagag ccatcagttg
     3001 atacctccag tgttggtacc aaaatcaatc gaaccgggaa taccatcagt tataccatca
     3061 tcagttggta tatgttcaag gaattgagta gacgtgtaca gactaagaaa agccagaaac
     3121 ctgcttaact tactgttttt atggactaaa gtagacgtta gtagacttga aaaaagcttt
     3181 gtatggtgcc gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa
     3241 ctgcgtctac cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt
     3301 atacctgtcg cacgccacca tgcaagcgcc agcttgctcg cacctcgcta agtgctgaaa
     3361 ttttcagcat tgcatacagc gatgtgtaac ctttgtcaca ctccaggcac cccgccctgc
     3421 catgctctac acttcccaga caacaccaga gaaggaccaa aaaatgtcga tgataaaaag
     3481 ctatgccgca aaagaagcag gcggcgaact ggaagtttat gagtacgatc ccggtgaact
     3541 gaagccacaa gatgttgaag tgcaggtgga ttactgcggg atctgccatt ccgatctgtc
     3601 gatgatcgat aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat
     3661 tggtcgcgtg gtggcgctcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg
     3721 tgtcgggatt ggctggacgg cgcgtagctg cgggcattgc gatgcctgta ttagcggtaa
     3781 tcagatcaac tgtgagcaag gtgcggtgcc gacgattatg aatcgcggtg gctttgccga
     3841 gaagttgcgt gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc
     3901 cgccgggccg ctgttgtgcg gtggtatcac ggtcttcaaa ccactgttaa tgcaccatat
     3961 cactgccacc agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa
     4021 acttctgcac gcaatgggat gcgaggtgac agcctttagt tcgaatccgg cgaaagagca
     4081 ggaagtactg gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa
     4141 agcactggcg gggcagtttg atctcattat caacaccgtc aacgtcagcc tcgactggca
     4201 gccttatttt gaagcgctga cgtacggcgg taatttccat acggtcggtg cggttctcac
     4261 gccgctgtct gttccggcct ttacgttaat tgcgggcgac cgcagcgtct ctggttctgc
     4321 taccggcacg ccttatgagt tgcgtaagct gatgcgcttt gccgcccgca gcaaggttgc
     4381 gccgactacc gaattgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga
     4441 tggtaaagcg cgctaccgag tggtcctgaa agccgacttc tgatcccttt gccgggtgcc
     4501 tgtcgcccgg cacttaaatc tctttggaag gcgtctcgca atctgctgaa tttagcggtg
     4561 aagagtgcgc agccgttgcc tatactccag gcaggaaact ggaggaaatc tcatgagcga
     4621 acccctgtta attgcccgca cgccggacac agaactgttt ttactgccgg gaatggcgaa
     4681 ccgtcacggg ctgattaccg gcgcaacggg gacgggtaaa accgttacgc tgcaaaaact
     4741 ggcagagtca ttgtcggaaa tcggcgtgcc ggtgtttatg gctgatgtga aaggcgatct
     4801 gaccggtatc gcgcaggcag gaacggcgtc ggaaaaactg ctcgcaaggc ttaaaaatat
     4861 cggcgtcaat gattggcaac cgcatgccaa tccggtggtg gtgtgggata tctttggcga
     4921 gaaaggccat ccggtgcggg cgacggtttc ggatctggga ccgctgttgc tggcgcgact
     4981 gttgaatctc aacgatgtgc aatctggcgt gctggatatc attttccgca ttgctgacga
     5041 tcaggggctg ttactgctcg actttaaaga tctgcgggca attacccagt acatcggcga
     5101 taacgccaaa tccttccaga atcagtacgg aaatatcagt agcgcatcgg ttggtgccat
     5161 ccagcgcggg ctgttgtcgc tggaacagca aggcgcagca cacttctttg gtgagccgat
     5221 gctggatatc aaagactgga tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct
     5281 cagcgccgag aaactttatc agatgccgaa actgtacgcc gccagcctgc tgtggatgct
     5341 ttcagagttg tatgaacaat tgccggaagc gggcgatctg gagaaaccaa aactggtgtt
     5401 tttcttcgac gaggcacatc tgctatttaa cgatgccccg caggtactgc tggataagat
     5461 tgagcaggtg ataaggctta ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa
     5521 cccgtctgat attccagata atgtgctcgg ccagctcggt aatcgcgttc aacacgcttt
     5581 gcgtgctttt acgcccaaag atcagaaagc ggtaaaagct gcggcgcaaa ccatgcaccc
     5641 cacccctgca tttgatacgg aaaaggcaat ccaggaactg gggaccggcg aggcgttaat
     5701 ctcgtttctc gatgcaaaag gaagtccttc tgtggtggaa cgggcgatgg tgattgcgcc
     5761 ttgttcgcgg atgggaccgg tgacggaaga tgagcgtaat ggcttgatta atcactctcc
     5821 ggtgtatggc aagtacgagg atgacgtgga ccgggaatcc gcctatgaga tgttgcaaaa
     5881 aggcgtacag gcaagcaccg agcagcaaaa taatcccccc gcgaaaggga aagaggtagc
     5941 ggtagatgac ggcattcttg gtggattgaa ggatattttg ttcggcacta ccggaccacg
     6001 cggcgggaag aaagatggtg ttgtgcaaac aatggcgaaa agcgccgccc gccaggtgac
     6061 gaatcagatt gtgcgtggaa tgttggggag tttgctgggg gggagaaaaa ggtaagccag
     6121 gtgcgatttg tctttgcctg atgcgacgct ggcgcgtctt attatggcca caccactgca
     6181 atatattgaa ttttaatgat ttttcataaa tgaacgaagc acactatgct aacaataata
     6241 aagggagctt tcgctccctt tattcgttca ttcggttacg attttctcat taacagccac
     6301 aggctgatta agaagaagct agcgcttggc aacagtgcgc cgatgatcgg cgggatgcca
     6361 taaaccaacg tcagcgggcc gaagatctgg tccagtacgt agaagacaaa accgaagcta
     6421 ataccggtga ccacacgcac acccatcggt acgctacgca gtgggccaaa gatgaacgac
     6481 agcgccatca gcatcatcac cgccacggat agcggctgga agattttgct ccacatgttg
     6541 agctgataac gcccggcatc ctgaccgctc gacttcagat acttcacgta gttgtgcaaa
     6601 ccactgatgg agagtgcatc cgggtccagc gccaccacgc ccagtttgtc cggcgtgagg
     6661 ttggttttcc aggtgccgct caccgtctgc gaaccggtga tctgtttcgg attggtcaga
     6721 tcagattcat caacctgcga cagacgccag actttatgtt ccgggtcaaa cttcgcggta
     6781 gcggcatagc gtacggattg cagacgacga ttctcgttaa aggcataaat actgatgcca
     6841 cctaactctt cgtcaccttt aacccgctca atgtagacga agttgttgcc atctttcgcc
     6901 cataaacctt gctgggtaga gagcaacgag ccgccgtaca tcgcctgcgc acggtagtta
     6961 cgcgccatct gctcgccctg cggcgcgacc cattcaccaa tcgccatcgt cagtaagacc
     7021 agcggaatgg cggttttcat caccgacagc gccacctgca tacgggtaaa accagaagcc
     7081 tgcatcacca ccagttcgct gcgctgcgcc agcatcccaa gaccaagcaa cgccccaagc
     7141 agagccgcca tcgggaagaa gatctgcaca tctttcggca cgctcagcaa ggtatacatt
     7201 cctgcgccta acgcgtcgta actcccctgc ccggcttttt tcaactgatc gacaaatttg
     7261 ataatgcccg acagcgacac cagcatgaac agtgtcatca tgatggtggt gaaaatagtt
     7321 ttaccgatat agcggtcaag tacgccaaaa ggttgcatca caccgctcct ttacgcgaaa
     7381 aactggcgcg caggcggcgg accggcacgg tgtcccaaag gttgagaaca atcgccaaag
     7441 ccagataaat caggttaacg gtccacatcc acagcgtcgg gtccagctta cctttaccgc
     7501 cgttcgattt cagggaggtc tggatcagga agaacaacag atacagcagc atggctggca
     7561 gcatcgacag tacgcgtccc tgacgtgggt taaccacgct cagcggtacg accataagtg
     7621 ccatcataaa cacggtgaat accaacgtga tacgccagtt cagttctgcg cgagcacgat
     7681 cggtgtcagt gttccacaat gtgcgcatgt ccatctggtc ggtatcgttc gggtcgagca
     7741 ccaccgcctg gtgaccaatg atcgcctgat aatcctggaa atccgtaatg cggaaatcac
     7801 gtaacagtgc agtgccttcg aagcgcgttc cctggttgag agtgacgacc tgggagccgt
     7861 cgcgcagctg ggttaaatgt ccggaatcgg ccaccaccac agaaggacgt gcattacctt
     7921 ttggtcgaat ttgcgcgagg aacacatctt tgaaatcgct gccgtcaacg ctttcgatga
     7981 acagcaccga gctgccatta gtcgcttgct ggaattgccc ttgcgccagc gccgccatgc
     8041 cagggttcgc tttcgcttct gctaacactt catcctgatg acgcgatgac cacggtcccg
     8101 cccacatcac gttaaccgcc gcgacgattg ccgtgaatac cgcaaggatc attgccgctt
     8161 tcaccagaac cgctttgctc aggccgcagg catgcattac cgtaatttca ctttcggtat
     8221 acagtttgcc cagcgtcatc agcagcccga ggaacaggct taatggcagg ataagctgcg
     8281 ccatttccgg cacgcccaac ccgagaaggg agagcaccag attcgccgga atatcgccgt
     8341 caaccgctgc gccgaggatc ctcactaact tttgacagaa gaagatcaaa agcaagatga
     8401 agagtatcgc cagctggctt ttgagcgtct cccgcaccag atatcttatg attatcactt
     8461 taaatacgcc cgtaaaaact cgtcttttgc aggattttag cttgtttcat ggcttaaacg
     8521 tcatttattc tcttgagtcg tcgaaatcgt cgctaagata attatactca acggattcac
     8581 ctctcagatt ttgttctgac gtgccaatgc cgtaataacg ttaagattaa cacgaagtca
     8641 tcgcaacagc ggacatgagt tacgaaagct tgcaattcta tctgtagcca ccgccgttgt
     8701 ctttaagatt caggagcgta gtgcatggag tttagtgtaa aaagcggtag cccggagaaa
     8761 cagcggagtg cctgcatcgt cgtgggcgtc ttcgaaccac gtcgcctttc tccgattgca
     8821 gaacagctcg ataaaatcag cgatgggtac atcagcgccc tgctacgtcg gggcgaactg
     8881 gaaggaaaac cggggcagac attgttgctg caccatgttc cgaatgtact ttccgagcga
     8941 attctcctta ttggttgcgg caaagaacgt gagctggatg agcgtcagta caagcaggtt
     9001 attcagaaaa ccattaatac gctgaatgat actggctcaa tggaagcggt ctgctttctg
     9061 actgaactgc acgttaaagg ccgtaacaac tactggaaag tgcgtcaggc tgtcgagacg
     9121 gcaaaagaga cgctctacag tttcgatcag ctgaaaacga acaagagcga accgcgtcgt
     9181 ccgctgcgta aaatggtgtt caacgtgccg acccgccgtg aactgaccag cggtgagcgc
     9241 gcgatccagc acggtctggc gattgccgcc gggattaaag cagcaaagga tcttggcaat
     9301 atgccgccga atatctgtaa cgccgcttac ctcgcttcac aagcgcgcca gctggctgac
     9361 agctacagca agaatgttat cacccgcgtt atcggcgaac agcagatgaa agagctgggg
     9421 atgcattcct atctggcggt cggtcagggt tcgcaaaacg aatcgctgat gtcggtgatt
     9481 gagtacaaag gcaacgcgtc ggaagatgcg cgcccaatcg tgctggtggg taaaggttta
     9541 accttcgact ccggcggtat ctccatcaag ccttcagaag gcatggatga gatgaagtac
     9601 gatatgtgcg gcgcggcggc ggtttacggc gtgatgcgta tggtcgcgga gctacaactg
     9661 ccgattaatg ttatcggcgt gctggcaggt tgtgaaaaca tgccaggcgg tcgcgcctat
     9721 cgtccgggcg atgtgttaac caccatgtct ggtcaaaccg ttgaagtgct caacaccgac
     9781 gctgaaggcc gcctggtact gtgcgacgtg ttaacttacg ttgagcgttt tgagccggaa
     9841 gcggtgattg acgtggcgac gctgaccggt gcctgcgtga tcgcgctggg tcatcacatt
     9901 actggtctga tggcgaacca caatccgctg gcccatgaac tgattgccgc gtctgaacaa
     9961 tccggtgacc gcgcatggcg cttaccgctg ggtgacgagt atcaggaaca actggagtcc
    10021 aattttgccg atatggcgaa cattggcggt cgtcctggtg gggcgattac cgcaggttgc
    10081 ttcctgtcac gctttacccg taagtacaac tgggcgcacc tggatatcgc cggtaccgcc
    10141 tggcgttctg gtaaagcgaa aggcgcaacc ggtcgtccgg tagcgttgct ggcacagttc
    10201 ctgttaaacc gcgccgggtt taacggcgaa gagtaagttt gcgttttacc ctcaccctaa
    10261 ccctctccct aagggagagg gaaccgatcg agcgcaatag aaattcattg ctcatctttt
    10321 agggtgagga atgcagcaat atttaaatcc accacaagaa gccccattta tgaaaaacgc
    10381 gacgttctac cttctggaca atgacaccac cgtcgatggc ttaagcgccg ttgagcaact
    10441 ggtgtgtgaa attgccgcag aacgttggcg cagtggtaag cgcgtactca tcgcctgtga
    10501 agatgaaaag caggcttacc ggctggatga agccctgtgg gcgcgtccgg cagaaagctt
    10561 tgttccgcat aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg agatcgcctg
    10621 gccgcaaaaa cgcagcagca gcccgcgcga tatattgatt agtctgcgaa caagctttgc
    10681 agattttgcc accgctttca cagaagtggt agacttcgtt ccttatgaag attctctgaa
    10741 acaactggcg cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc tgaatacggc
    10801 aacctggaaa taatggaaaa gacatataac ccacaagata tcgaacagcc gctttacgag
    10861 cactgggaaa agcagggcta ctttaagcct aatggcgatg aaagccagga aagtttctgc
    10921 atcatgatcc cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc cttccagcaa
    10981 accatcatgg ataccatgat ccgctatcag cgcatgcagg gcaaaaacac cctgtggcag
    11041 gtcggtactg accacgccgg gatcgctacc cagatggtcg ttgagcgcaa gattgccgca
    11101 gaagaaggta aaacccgtca cgactacggc cgcgaagctt tcatcgacaa aatctgggaa
    11161 tggaaagcgg aatctggcgg caccattacc cgtcagatgc gccgtctcgg caactccgta
    11221 gactgggagc gtgaacgctt cactatggac gaaggcctgt ccaatgcggt gaaagaagtt
    11281 ttcgttcgtc tgtataaaga agacctgatt taccgtggca aacgcctggt aaactgggat
    11341 ccgaaactgc gcaccgctat ctctgacctg gaagtggaaa accgcgaatc gaaaggttcg
    11401 atgtggcaca tccgctatcc gctggctgac ggcgcgaaaa ccgcagatgg taaagattat
    11461 ctggtggttg cgactactcg tccggaaacc ctgctgggcg ataccggcgt ggccgttaac
    11521 ccggaagatc cgcgttacaa agatctgatt ggcaaatatg tcattctgcc gctggttaac
    11581 cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac cggctgcgtg
    11641 aagatcaccc cggcgcacga ctttaacgac tatgaagtcg gtaaacgtca cgccctgccg
    11701 atgatcaaca tcctgacctt tgacggcgat atccgtgaaa gtgcccaggt gttcgatacc
    11761 aaaggtaacg aatctgacgt ttattccagc gagatccccg cagaattcca gaaactggag
    11821 cgttttgctg cacgtaaagc ggtcgttgct gcggttgacg cactcggcct gctggaagaa
    11881 attaaaccac atgacctgac cgttccttat ggcgaccgtg gtggcgtggt catcgaaccg
    11941 atgctgaccg accagtggta cgtgcgtgcc gatgtcctgg cgaaaccggc agttgaagcg
    12001 gttgagaacg gcgacattca gttcgtaccg aagcagtacg aaaacatgta cttctcctgg
    12061 atgcgcgata ttcaggactg gtgtatctct cgtcagttgt ggtggggtca ccgtatcccg
    12121 gcatggtatg acgaagcggg taacgtttat gttggccgca acgaagacga agtgcgtaaa
    12181 gaaaataacc tcggtgctga tgttgtcctg cgtcaggacg aagacgttct cgacacctgg
    12241 ttctcttctg cgctgtggac cttctctacc cttggctggc cggaaaacac cgacgccctg
    12301 cgtcagttcc acccaaccag cgtgatggta tctggtttcg acatcatctt cttctggatt
    12361 gcccgcatga tcatgatgac catgcacttc atcaaagatg aaaatggcaa accgcaggtg
    12421 ccgttccaca ctgtttacat gaccggtctg attcgtgacg acgaaggcca gaagatgtcc
    12481 aaatccaagg gtaacgttat cgacccactg gatatggttg acggtatttc gctgccagaa
    12541 ctgctggaaa aacgcaccgg caatatgatg cagccacagc tggcggacaa aatccgtaag
    12601 cgcaccgaga agcagttccc gaacggtatt gaaccgcacg gtactgacgc gctgcgcttc
    12661 accctggcgg cgctggcttc taccggtcgc gatatcaact gggatatgaa acgcctggaa
    12721 ggttaccgta acttctgtaa caagctgtgg aacgccagcc gctttgtgct gatgaacacc
    12781 gaagatcagg attgcggctt caacggcggc gagatgacgc tgtcgctggc ggatcgctgg
    12841 attctggcgg agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga cagcttccgc
    12901 ttcgatatcg ccgcaggcat tctgtatgag ttcacctgga accagttctg tgactggtat
    12961 ctggagctga ctaagccggt aatgaacggt ggcaccgaag ccgaactgcg cggtactcgc
    13021 catacgctgg tgaccgtgct ggaaggtctg ctgcgcctcg cgcatccgat cattccgttc
    13081 atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc cgacaccatc
    13141 atgctgcagc cgttcccgca gtacgatgca tctcaggttg atgaagccgc actggccgac
    13201 acagagtggt tgaagcaggc gatcgttgcg gtacgtaaca tccgtgcaga aatgaacatc
    13261 gcgccgggca aaccgctgga gctgctgctg cgtggttgca gcgcggatgc agaacgtcgc
    13321 gtaaatgaaa accgtggctt cctgcaaacc ctggcgcgtc tggaaagcat caccgtgctg
    13381 cctgccgatg acaaaggtcc ggtttccgtt acgaagatca tcgacggtgc agagctgctg
    13441 atcccgatgg ctggcctcat caacaaagaa gatgagctgg cgcgtctggc gaaagaagtg
    13501 gcgaagattg aaggtgaaat cagccgtatc gagaacaagc tggcgaatga aggctttgtc
    13561 gcccgcgcac cggaagcggt catcgcgaaa gagcgtgaga agctggaagg ctatgcagaa
    13621 gcgaaagcga agctgattga acagcaggct gttatcgccg cgctgtaatc aaaacacagt
    13681 gatgaaaacg aaggccggag catgctccgg ccttttttat ctcttacaga aatggtaaag
    13741 aaggcataat tccccgtgaa atactggcta agaagccttt atctggttgt tcttctttat
    13801 tgattaaagg aaggtcatcc aaatcgccca gcaaatatgt attttttgcc tgccagtcaa
    13861 tcatccatat tttcagcagt ggataagcca gaccgcccgt aatcccggat atcaccacca
    13921 acgcacacat gcgataaagc ataccgtggt atgttaaagt tgagcgaaaa cgaatacgcc
    13981 catcattcag tgacaggttg ctcataaaat gatttcgcaa cgacaccgtt aaataactgg
    14041 tgctaacagc aatcccaaaa taatagatta actgcgcgat tatcatttta cgttgcattt
    14101 ccataaattg ctgtaaattc tcaatatcat catttgcata cacagaacta tcatacgcat
    14161 ttaatatttg atcgaagata atataaccag caacagcgag aaaaggtaat aaagctaaaa
    14221 atgcgagaat ggcatatttt atacaatacg tagtatctaa tttcaccttg aaacgatgta
    14281 taccgaaact ggtattgctc cagagaaaac tcattaccag actatataaa gtaccattaa
    14341 aaataccaat ggaaacaata ccaactattg ccatcagaac cacagatata ataacactac
    14401 ttgaactatt ggcatgtagc atctttgtcg agataaagaa aacagccccc atcccaatgg
    14461 ccattaaaat cggtaagaaa aaggttaccc accagaaccc tttcaaagag caattaaaac
    14521 taaatcttac gccgttgaga ctggtcatca aggcctgata acgcagtcct tttgctgcca
    14581 taaatataag caaaaccaac agtgacaaag tcaaaacaca gccaacaagc ggcatatctg
    14641 ctgacactgt cattaagatt gcgaaataga aaaaaacaaa aacaagacaa ctaacaaaaa
    14701 cattcccacc ggtaattcca taagaaaatc gttgtccgtt aacttccata ttagcataga
    14761 gataacgctt acatttcatt aatgcccatg gtaaatagat acctagcgtt ataatcgtta
    14821 acaacacatt caccacacaa ataagaaaat atctctcacc tgtgccacga aaaacaaacg
    14881 aatgggacgg aacatccatt tcattaataa cttgagccat aaaaattccc tgaattaaaa
    14941 aatccattga tataatttat atatacagac agtaaaatgt tctgccacgt tacaatactc
    15001 ttatcagtaa taggctggca aaaactttat tactttataa gtcgcttgcg cacttaagaa
    15061 gagagtggta ttaaacaaat gtataattac tttattttta ttgggttaaa ctatgaataa
    15121 cattgcgccg caatcacctg taatgcgtcg cctgacgctg caggataatc ccgctatcgc
    15181 ccgcgtcatt cgtcaggtat ccgccgaata cggtcttacc gctgataaag gctacaccgt
    15241 cgccgatccg aaccttgacg agttgtatca agtgtatagt cagcctggcc atgcatattg
    15301 ggtggttgag tacgatggtg aagtggtcgg cggcggtggg atagcgccat tagcaggtag
    15361 tgaatcggac atttgcgaac tgcaaaagat gtattttctt ccggctattc gcggtaaagg
    15421 gctagcgaaa aaactggcct taatggcgat ggagcaggca cgagagatgg gcttcaaacg
    15481 ctgctatctg gaaacgaccg cttttttaaa ggaagccatt gcgctttatg agcatttggg
    15541 ctttgagcat atcgactatg cgcttggctg cacgggccat gtagattgtg aagtgcggat
    15601 gctacgcgaa ctctaaaaaa atgcccgcta gtgtgtgcta tcgggcattt taatcaatta
    15661 attagtggcg aaccccgtca tcgtcttcat cgacaaaatc ttcatcgtcg ccgtcttcgc
    15721 cgttaggatc ttcaaagtaa gtgccccaac cgtcgtactc aacgtcaaat ttctccgcca
    15781 gcgtcatcag ttgttcaacc tgggcatcga tcagatcggc attcaacgcg cactcgctga
    15841 ggatgtcgca gcaaatcacg atatcaccat cttcaacttc cagctcttct ggatcggtca
    15901 cttcgtaacc gagtttaaac gcttcaactg ctgctttttc cagggtttcc agatcgtctg
    15961 cggaaagatg gtgttcgatg gtgtacagcg cgtccggatc gctgccatct tccagtaatt
    16021 cttcaataat caaacgtgtt tcttcacgct gttcttccag ttgttccggg tttgccatgg
    16081 ctcattcctc ttaatgtcct gccgatactc ttattgtcac acaccactga cattgcctcc
    16141 acctttgtga caaagattta tgctttagac ttgcaaatga ataatcatcc atataaattg
    16201 aattttaatt cattaaggcg ttagccacag gagggatcta tgtccgggtt ttatcataag
    16261 catttcctga aattactcga tttcacgcca gctgaactca acagcctgct gcagttagcc
    16321 gcgaagctga aagccgataa gaaaagcggc aaagaagaag ccaaactcac tggtaaaaac
    16381 atcgcgctca tcttcgaaaa agactcgacc cgtacccgat gctctttcga agttgccgca
    16441 tatgaccagg gtgctcgcgt tacttatctc ggcccaagcg gcagtcagat tggtcataaa
    16501 gagtcgatta aagacactgc ccgcgtgctg ggtcgcatgt atgacggtat tcagtatcgc
    16561 ggctatggtc aggagattgt cgaaacactg gcggaatacg ctggcgtgcc ggtatggaac
    16621 ggcctgacca atgagtttca ccccacgcag ctgctggcgg atcttctcac catgcaagag
    16681 catttgcccg gtaaagcgtt caacgaaatg acgctggtct atgcaggtga cgcacgtaac
    16741 aacatgggca attcgatgct cgaagctgcg gcgcttaccg gtctggattt gcgtctggtc
    16801 gcgccacagg catgctggcc ggaagctgcg ctggttacgg aatgccgcac cctggcacag
    16861 caaaatggcg ggaatattac gctgacggaa gatgtagcca aaggagttga aggtgctgac
    16921 tttatctata ccgatgtatg ggtgtcgatg ggggaagcaa aagagaaatg ggcagaacgg
    16981 attgcattac tgcgtgatta tcaggtgaac agcaagatga tgcagttgac cggtaacccg
    17041 gaggtcaaat tcctccactg cctgcccgcg tttcatgacg accagacgac gcttggtaag
    17101 aaaatggcgg aagagtttgg cctacatggc ggaatggaag tgactgatga ggtcttcgaa
    17161 tctgccgcca gcattgtttt tgatcaggcg gaaaaccgca tgcataccat caaagcggtg
    17221 atggtcgcga cgctcagtaa ataattgata tcgctataaa gatgccggga aatacaaaaa
    17281 catcccggca tcacattatt ttatgcagta atagttcaat tattccatcc atatttttct
    17341 attgctatac gtaaatcttc c
//
LOCUS       DAOFQD010000102        17196 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.102, whole genome shotgun sequence.
ACCESSION   DAOFQD010000102 DAOFQD010000000
VERSION     DAOFQD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.102"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            260..346
                     /gene="leuL"
                     /locus_tag="QBF46_003464"
     CDS             260..346
                     /gene="leuL"
                     /locus_tag="QBF46_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDQ6675604.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            439..2010
                     /gene="leuA"
                     /locus_tag="QBF46_003465"
     CDS             439..2010
                     /gene="leuA"
                     /locus_tag="QBF46_003465"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDQ6675605.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDINVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITEYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2010..3101
                     /gene="leuB"
                     /locus_tag="QBF46_003466"
     CDS             2010..3101
                     /gene="leuB"
                     /locus_tag="QBF46_003466"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDQ6675606.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3104..4504
                     /gene="leuC"
                     /locus_tag="QBF46_003467"
     CDS             3104..4504
                     /gene="leuC"
                     /locus_tag="QBF46_003467"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDQ6675607.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPTMA
                     AAAAVTGHFADIRNIK"
     gene            4515..5120
                     /gene="leuD"
                     /locus_tag="QBF46_003468"
     CDS             4515..5120
                     /gene="leuD"
                     /locus_tag="QBF46_003468"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDQ6675608.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5169..6347)
                     /gene="setA"
                     /locus_tag="QBF46_003469"
     CDS             complement(5169..6347)
                     /gene="setA"
                     /locus_tag="QBF46_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HDQ6675609.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLILFHSRLALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVIALFLTAKVKDV
                     "
     gene            complement(6449..6580)
                     /gene="sgrT"
                     /locus_tag="QBF46_003470"
     CDS             complement(6449..6580)
                     /gene="sgrT"
                     /locus_tag="QBF46_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDQ6675610.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6669..8324
                     /gene="sgrR"
                     /locus_tag="QBF46_003471"
     CDS             6669..8324
                     /gene="sgrR"
                     /locus_tag="QBF46_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDQ6675611.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8358..8457
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8488..9471
                     /gene="thiB"
                     /locus_tag="QBF46_003472"
     CDS             8488..9471
                     /gene="thiB"
                     /locus_tag="QBF46_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDQ6675612.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9447..11057
                     /gene="thiP"
                     /locus_tag="QBF46_003473"
     CDS             9447..11057
                     /gene="thiP"
                     /locus_tag="QBF46_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDQ6675613.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFIEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGLNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADVIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11041..11739
                     /gene="thiQ"
                     /locus_tag="QBF46_003474"
     CDS             11041..11739
                     /gene="thiQ"
                     /locus_tag="QBF46_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDQ6675614.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAITRQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11853..12617)
                     /locus_tag="QBF46_003475"
     CDS             complement(11853..12617)
                     /locus_tag="QBF46_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6675615.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKNPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12703..13581)
                     /gene="araC"
                     /locus_tag="QBF46_003476"
     CDS             complement(12703..13581)
                     /gene="araC"
                     /locus_tag="QBF46_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDQ6675616.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13920..15620
                     /gene="araB"
                     /locus_tag="QBF46_003477"
     CDS             13920..15620
                     /gene="araB"
                     /locus_tag="QBF46_003477"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDQ6675617.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITGLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15631..17133
                     /gene="araA"
                     /locus_tag="QBF46_003478"
     CDS             15631..17133
                     /gene="araA"
                     /locus_tag="QBF46_003478"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDQ6675618.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNGLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
BASE COUNT         4107 a         4731 c         4574 g         3784 t
ORIGIN      
        1 gttttgcaac gcgtgcattt tgtccccttt ttcctcgttg attagatgca aaaatttatg
       61 ctgaaatatg tcaaccgatg aaaagcgtcg gtagttaagc agaaattaat atcgcttact
      121 ttaaccaccg cagcacaatt agctaatttt acggatgcag aactcacgct ggcgggacgt
      181 ttttattgcg tcagggttga catccgtttt tgtatccagt aactctaaaa gcatatcgca
      241 ttcatctgga gctgatttaa tgactcacat cgttcgcttt atcggtctac tactactaaa
      301 cgcatcttct ttgcgcggta gacgagtgag cggcatccag cattaagcca gcacgcagtc
      361 aaacaaaaaa cccgcgccat tgcgcgggtt tttttatgcc cgaagcgagg cgctctaaaa
      421 gagacaagga cccaaaccat gagccagcaa gtcattattt tcgatactac attgcgcgac
      481 ggtgaacagg cgttacaggc aagcttgagt gtgaaagaaa aactgcaaat tgcgctggcc
      541 cttgagcgta tgggtgttga cgtgatggaa gtcgggttcc ccgtttcttc gccgggtgat
      601 tttgaatcag tgcaaaccat cgctcgccag gttaaaaaca gccgcgtatg cgcgttagct
      661 cgctgcgtgg aaaaagatat caacgtggcg gccgaatccc tgaaagtcgc cgaagccttc
      721 cgtattcata cctttattgc cacttcgcca atgcacatcg ccaccaagct gcgcagcacg
      781 ctggacgagg tgatcgaacg cgctatctat atggtgaaac gcgcccgtaa ttacaccgat
      841 gatgttgaat tttcttgcga agatgccggg cgtacaccca ttgccgatct ggcgcgagtg
      901 gtcgaagcgg cgattaatgc cggtgccacc accatcaaca ttccggacac cgtgggctac
      961 accatgccgt ttgagttcgc cggaatcatc agcggcctgt atgaacgcgt gcctaacatc
     1021 gacaaagcca ttatctccgt acatacccac gacgatttgg gcctggcggt cggaaactca
     1081 ctggcggcgg tacatgccgg tgcacgccag gtggaaggcg caatgaacgg gatcggcgag
     1141 cgtgccggaa actgttccct ggaagaagtc atcatggcga tcaaagttcg taaggatatt
     1201 ctcaacgtcc acaccgccat taatcaccag gagatatggc gcaccagcca gttagttagc
     1261 cagatttgta atatgccgat cccggcaaac aaagccattg ttggcagcgg cgcattcgca
     1321 cactcctccg gtatccacca ggatggcgtg ctgaaaaacc gcgaaaacta cgaaatcatg
     1381 acaccagaat ctattggtct gaaccaaatc cagctgaatc tgacctctcg ttcggggcgt
     1441 gcggcggtga aacatcgcat ggatgagatg gggtataaag aaagtgaata taatttagac
     1501 aatttgtacg atgctttcct gaagctggcg gacaaaaaag gtcaggtgtt tgattacgat
     1561 ctggaggcgc tggccttcat cggtaagcag caagaagagc cggagcattt ccgtctggat
     1621 tacttcagcg tgcagtctgg ctctaacgat atcgccaccg ccgccgtcaa actggcctgt
     1681 ggcgaagaag tcaaagcaga agccgccaac ggtaacggtc cggtcgatgc cgtctatcag
     1741 gcaattaacc gcatcactga atataacgtc gaactggtga aatacagcct gaccgccaaa
     1801 ggccacggta aagatgcgct gggtcaggtg gatatcgtcg ctaactacaa cggtcgccgc
     1861 ttccacggcg tcggcctggc taccgatatt gtcgagtcat ctgccaaagc catggtgcac
     1921 gttctgaaca atatctggcg tgccgcagaa gtcgaaaaag agttgcaacg caaagctcaa
     1981 cacaacgaaa acaacaagga aaccgtgtga tgtcgaagaa ttaccatatt gccgtattgc
     2041 cgggggacgg tattggtccg gaagtgatga cccaggcgct gaaagtgctg gatgccgtgc
     2101 gcaaccgctt tgcgatgcgc atcaccacca gccattacga tgtaggcggc gcagccattg
     2161 ataaccacgg gcaaccactg ccgcctgcga cggttgaagg ttgtgagcaa gccgatgccg
     2221 tgctgtttgg ctcggtaggc ggcccgaagt gggaacattt accaccagac cagcaaccag
     2281 aacgcggcgc gctgctgcct ctgcgtaagc acttcaaatt attcagcaac ctgcgcccgg
     2341 caaaactgta tcaggggctg gaagcattct gtccgctgcg tgcagacatt gccgcaaacg
     2401 gcttcgacat cctgtgtgtg cgcgaactga ccggcggcat ctatttcggt cagccaaaag
     2461 gccgcgaagg tagcggacaa tatgaaaaag cctttgatac cgaggtgtat caccgttttg
     2521 agatcgaacg tatcgcccgc atcgcgtttg aatctgctcg caagcgtcgc cacaaagtga
     2581 cgtcgatcga taaagccaac gtgctgcaat cctctatttt atggcgggag atcgttaacg
     2641 agatcgccac ggaatacccg gatgtcgaac tggcgcatat gtacatcgac aacgccacca
     2701 tgcagctgat taaagatcca tcacagtttg acgttctgct gtgctccaac ctgtttggcg
     2761 acattctgtc tgacgagtgc gcaatgatca ctggctcgat ggggatgttg ccttccgcca
     2821 gcctgaacga gcaaggtttt ggactgtatg aaccggcggg cggctcggca ccagatatcg
     2881 caggcaaaaa catcgccaac ccgattgcac aaatcctttc gctggcactg ctgctgcgtt
     2941 acagcctgga tgccgatgat gcggcttgcg ccattgaacg cgccattaac cgcgcattag
     3001 aagaaggcat tcgcaccggg gatttagccc gtggcgctgc cgccgttagt accgatgaaa
     3061 tgggcgatat cattgcccgc tatgtagcag aaggggtgta atcatggcta agacgttata
     3121 cgaaaaattg ttcgacgctc acgttgtgta cgaagccgaa aacgaaaccc cactgttata
     3181 tatcgaccgc cacctggtgc atgaagtgac ctcaccgcag gcgttcgatg gtctgcgcgc
     3241 ccacggtcgc ccggtacgtc agccgggcaa aaccttcgct accatggatc acaacgtctc
     3301 tacccagacc aaagacatta atgcctgcgg tgaaatggcg cgtatccaga tgcaggaact
     3361 gatcaaaaac tgcaaagaat ttggcgtcga actgtatgac ctgaatcacc cgtatcaggg
     3421 gatcgtccac gtaatggggc cggaacaggg cgtcaccttg ccggggatga ccattgtctg
     3481 cggcgactcg cataccgcca cccacggcgc gtttggcgca ctggcctttg gtatcggcac
     3541 ttccgaagtt gaacacgtac tggcaacgca aaccctgaaa cagggccgcg caaaaaccat
     3601 gaaaattgaa gtccagggca aagccgcgcc gggcattacc gcaaaagata tcgtgctggc
     3661 aattatcggt aaaaccggta gcgcaggcgg caccgggcat gtggtggagt tttgcggcga
     3721 agcaatccgt gatttaagca tggaaggtcg tatgaccctg tgcaatatgg caatcgaaat
     3781 gggcgcaaaa gccggtctgg ttgcaccgga cgaaaccacc tttaactatg tcaaaggccg
     3841 tctgcatgcg ccgaaaggca aagatttcga cgacgccgtt gcctactgga aaaccctgca
     3901 aaccgacgaa ggcgcaactt tcgataccgt tgtcactctg caagcagaag aaatttcacc
     3961 gcaggtcacc tggggcacca atcccggcca ggtgatttcc gtgaacgaca atattcccga
     4021 tccggcttcg tttgccgatc cggttgaacg cgcgtcggca gaaaaagcgc tggcctatat
     4081 ggggctgaaa ccgggtattc cgctgaccga agtggctatc gacaaagtgt ttatcggttc
     4141 ctgtaccaac tcgcgcattg aagatttacg cgcggcagcg gagatcgcca aagggcgaaa
     4201 agtcgcgcca ggcgtgcagg cactggtggt tcccggctct ggcccggtaa aagcccaggc
     4261 ggaagcggaa ggtctggata aaatctttat tgaagccggt tttgaatggc gcttgcctgg
     4321 ctgctcaatg tgtctggcga tgaacaacga ccgtctgaat ccgggcgaac gttgtgcctc
     4381 caccagcaac cgtaactttg aaggccgcca ggggcgcggc gggcgcacgc atctggtcag
     4441 cccgacaatg gctgccgctg ctgctgtgac cggacatttc gccgacattc gcaacattaa
     4501 ataaggagca caccatggca gagaaattta tcaaacacac aggcctggtg gttccgctgg
     4561 atgccgccaa tgtcgatacc gatgcaatca tcccgaaaca gtttttgcag aaagtgaccc
     4621 gtacgggttt tggcgcgcat ctgtttaacg actggcgttt tctggatgaa aaaggccaac
     4681 agccaaaccc ggacttcgtg ctgaacttcc cgcagtatca gggcgcttcc attttgctgg
     4741 cacgagaaaa cttcggctgt ggctcttcgc gtgagcacgc gccctgggca ttgaccgact
     4801 acggttttaa agtggtgatt gcgccgagtt ttgctgacat cttctacggc aatagcttta
     4861 acaaccagct gctgccggtg aaattaagcg atgcagaagt ggacgaactg tttgcgctgg
     4921 tgaaagccaa tccggggatc catttcgacg tggatctgga agcgcaagag gtgaaagcgg
     4981 gagagaaaac ctatcgcttt accatcgatg ccttccgccg ccactgcatg atgaacggtc
     5041 tggacagtat tgggcttacc ttgcagcacg acgacgccat tgccgcttat gaagcaaaac
     5101 aacctgcgtt tatgaattaa tccccttgcc cggtcaaatg accgggcttt ccgctatcgt
     5161 ccacgtcatc aaacgtcttt aacctttgcg gttaaaaata atgcgataac agaaataacc
     5221 gcaattaccc agtagacagc aaagtgcccc caactttgtg caattgctcc ctgaataacg
     5281 ccagccagaa ttaccccggt agaaatactg ttagtaaata aggtggtagc tgcccccgct
     5341 cttccaggca ttaaatcctg aaaccatagc atcccaatac ccgcaacaat gccgataaat
     5401 acagcgttaa aaagttgcag cgtcatcaac gccagacggc tatgaaataa aatcaatccg
     5461 gtgtaaaaca gtactcctgc cgccactgct atgaccatca ttcgccgctt accataacgt
     5521 ttgacatagt agccagccag aatcattgct ggtatttcca gtccagctgc cgtccccatc
     5581 aggaaacccg ccagtttgtc tggcaatcct aactcgctac tgatccacaa cggcatatca
     5641 ataatgtaca tggtgttgca ggtccacatt aacgtcgagg cgacaaataa catccgtacg
     5701 ttactatcct gccagccacc ttgcattgat aaagcatttt ccgacggcag ttctacccgc
     5761 gccacagacg gaagcataaa tgcaatcaat accagactga gtgtgaatat cccggcggca
     5821 atcgaaaaca tcaccgtaaa gccgtaattc aacgccagca taaaggccaa cggtggaccg
     5881 ataacccatg ccagagaaag ctgcgcacgc atcaccgagc taaacatcac cacttctcgc
     5941 gccgagttat ccgcatattc ccgcgccaga gcaaataact gtggcattgc cgtattggcc
     6001 agagatgcca gaagcacacc acaggtgata agcgtcagat aatgacgatt aaatgcaaac
     6061 aatagcgcat tgccgatagc catcaaacag caaaatataa tcagttttcg ccgatcgccc
     6121 tgactgtcag aacgttttgc caaccagagg cttacgccga tcccagcaat agcattcacc
     6181 gtataaaaga ggccgatcca gaaaggttgc gcgccaacct cacgactcag aaataagctc
     6241 aatgtaggag cctgtagcgc cccggccacc cccatcataa aagcgaccag cataaatgcc
     6301 gcgtaaacac cgttcatacg gcgagccatc gtcattatcc agatcatacg ttcccttttt
     6361 agcgcggcga gaataaaaaa aaccagcagg tataatctgc tggcgggtga ttttacacca
     6421 atactcagtc acacatgatg caggcaagtc aactttcaga attgcggtca tcccactgca
     6481 tcagtccttc aagcatggtt aatcgttgtg ggacgcttaa ccaacgcaac cagcacaact
     6541 tcgctgtcgc ggtaaaatag tgctgataaa actgacgcat ggggcacccc cttgcttcat
     6601 cgttaggaat tattgactta atatagggaa aataaaattg ctgtcttttg cacaggagtt
     6661 ccccttttat gccatctgct cgtctgcaac aacagttcat ccgcctgtgg caatgctgcg
     6721 agggtaaatc gcaggacaca acgctcaacg aactggcagc gttattgagc tgctcgcgtc
     6781 gtcatatgcg caccctgctc aacaccatgc aggatcgcgg ctggctgacg tgggaagcgg
     6841 aagtcgggcg tggtaaacgc tcgcgtctga cattcctcta taccgggctg gcgcttcagc
     6901 aacagcgggc ggaagacctg ctggagcagg atcgtatcga ccaactagtg cagttggttg
     6961 gcgacaaagc gactgtgcgg caaatgctgg tttctcatct gggccgcagc ttccgccagg
     7021 ggcggcacat cctgcgcgtg ctctactatc gtccgttgcg taatctgcta cctggcagcg
     7081 cattgcgccg ttccgaaacc catattgccc ggcaaatctt cagttcgcta acgcgcataa
     7141 atgaggaaaa tggggaactg gaagcagaca tcgcccacca ctggcagcaa atatcaccgc
     7201 ttcactggcg tttctttttg cgtccaggag tccattttca tcatggtcgt gaactggaaa
     7261 tggacgacgt gatcgcctct ttaaaacgaa tcaatacgct gccgctctat tcgcatattg
     7321 ctgacattgt gtcgccgacg ccctggacgc tggatatcca tctcacgcaa ccggaccgct
     7381 ggttaccgtt attgctgggg caagttccgg cgatgatcct gccgcgcgaa tgggaaaccc
     7441 tcagtaactt tgccagccat cccatcggca ccggtccgta tgcggtgatc cgcaacagca
     7501 ccaatcaact gaaaattcag gcattcgatg acttcttcgg ttaccgggca ttaatcgacg
     7561 aagttaacgt ctgggttctg ccggaaattg ccgacgagcc agctggaggt ctgatgctaa
     7621 aaggtccaca gggtgaggaa aaagagattg aaagccgcct ggaggaaggt tgctactatt
     7681 tactattcga cagccgcacc catcgcgggg cgaatcagca agtcagggac tgggtaagct
     7741 atgtgctttc tccaactaat ctggtctatt tcgctgagga acagtaccag caactgtggt
     7801 tcccggctta tggactgctc ccccgttggc accatgcccg caccataaag agcgaaaaac
     7861 cggctggcct ggaaagcctc atcctgacct tttatcagga tcacagtgag catcgggtga
     7921 ttgccgggat catgcagcag attctggcaa gtcaccaggt cacgctggaa atcaaagaga
     7981 tcagctacga tcagtggcat gaaggagaga tcgagagcga tatctggctt aacagcgcca
     8041 actttacgct gccgctggat ttttcgctgt tcgcgcactt gtgcgaggtg ccgctgctcc
     8101 aacactgtat tccgatcgac tggcaagccg acgccgcccg ctggcgcaat ggcgaaatga
     8161 acctggcgaa ctggtgccag caactggtcg ccagcaaagc aatggtgccg cttatccacc
     8221 actggctgat cattcagggg caacgcagta tgcgcggcct gcgcatgaac accctcggct
     8281 ggttcgattt taaatcagcg tggtttgcgc caccggatcc atgattgctg gtagataaca
     8341 aaatcactac actaacgccg ttctcaacgg ggtgccacgc aaacgcgtgc gctgagaaaa
     8401 tacccgtcga acctgatccg gataacgccg gcgaagggat ttgaggctcc ttctcaagtc
     8461 ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat gtcttcccct gctgttgctg
     8521 tgcacagcgc ccgttttcgc taaacccgtt ctgactgttt atacctacga ttccttcgcc
     8581 gccgactggg ggcctggtcc aaagatcaaa aaagcttttg aagccgactg taactgcgaa
     8641 ctgaaactgg tggcgctgga agatggcgtt tcgcttctca accgtctacg gatggaaggc
     8701 aaaaatagta aagccgatgt ggtgctgggg ctggataaca atctgttaga cgccgccagt
     8761 aaaaccggac tatttgccaa aagcggtgtg gcagcggatg ccgttaacgt tcccggcggc
     8821 tggaataatg acactttcgt cccgtttgat tacggctatt tcgccttcgt ctatgacaag
     8881 aacaaactga aaaacccgcc gcaaagcctg aaagaattgg ttgagagcga tcagaactgg
     8941 cgggtgattt atcaggatcc gcgcaccagt acgccggggc tgggcctgct gttgtggatg
     9001 caaaaagtct atggcgatga cgccccacaa gcctggcaaa aactggcgaa gaaaaccgtc
     9061 acggtcacca aaggctggag cgaagcctac ggcctgtttt taaaaggtga aagcgatctg
     9121 gtactgagtt acaccacctc tccggcttat cacattctcg aagagaaaaa agataactac
     9181 gccgccgcga actttagcga aggtcactat ctgcaggtgg aagtcgccgc ccgcaccgct
     9241 gccagcaagc agccggagct ggcgcaaaaa ttcctccagt ttatggtttc tccggctttt
     9301 cagaatgcga tcccaaccgg taactggatg tatccggtgg caaatgtcac gctgcctgcc
     9361 gggtttgaac aattgaccaa accggccacc acgctggagt tcacaccagc tgaagtggcg
     9421 gcacaacgtc aggcatggat tagcgaatgg caacgcgccg tcagccgtta attcccggct
     9481 ggttaattcc aggtgtaagc gccgccacgc tggtggtagc ggttgcgctc gcggcgtttc
     9541 tcgccctgtg gtggaacgcg ccgcagggta actgggtggc agtctggcag gacagctacc
     9601 tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta ctctctgtcg
     9661 tacccgcgat attcctcgcc cgcgccctct atcgcaggcg ctttccgggt cggctggcgc
     9721 tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct ggttgctgtt ttcggcattc
     9781 ttagcgtcta tggtcgtcag ggctggctgg catcgctctg ccaatcgctc ggtctggagt
     9841 ggaccttttc gccctacggc ctgcaaggca ttttgctggc ccatgtgttt tttaatctgc
     9901 caatggcgag ccgcttatta ctccaggcac tggaaaacat tcccggcgaa caacgtcagc
     9961 ttgccgccca gcttgggatg cgcggctggc attttttccg cttcatcgaa tggccgtggc
    10021 tacggcgaca aatcccaccg gttgccgcgc ttatctttat gctctgtttc gccagcttcg
    10081 ccaccgtgct gtcgctgggc ggcggtccgc aagcgaccac tatcgagctg gctatttatc
    10141 aggcgctgag ttacgactac gatcctgccc gcgcggcgat gctggcgctg atccagatgg
    10201 tgtgttgcct tgggctggtg ctgttgagtc agcgattgag taaggccatt gcgccaggca
    10261 ccacgctgct gcaaggctgg cgcgacccgg acgatcgtct gcatagccgc atttgcgaca
    10321 cggtgttaat tgtgctggcg ctgctgctgt tgctgccacc gttgctggcg gtgatcgtcg
    10381 atgggctaaa tcgccagttg ccggaagtgc tggcacaacc ggtgctgtgg caggcgctgt
    10441 ggacctcgtt gcgtattgcg ctggcggcag gtgtattgtg cgtagtgctg accatgatgc
    10501 tgctatggag cagtcgcgaa cttcgggcgc ggcagaaaat gctggcgggt caggcgctgg
    10561 agatgagcgg catgttgatc ctcgccatgc cggggattgt gctggctacc ggcttctttt
    10621 tactgctcaa caacaccatt ggcctgccgc aatctgctga cgtcattgtg attttcacca
    10681 atgcgttaat ggcgatccct tacgcgctga aggtgctgga aaacccgatg cgcgatatca
    10741 ccgcccgcta cagtatgttg tgtcagtcgc tgggcattga aggctggtcg cgcttaaaag
    10801 tggtcgagct gcgcgccctg aaacgtccac tggcgcaggc gctggctttt gcctgcgtgc
    10861 tgtcgattgg tgattttggc gtagtggcgt tgttcggtaa cgatgatttc cgtaccctgc
    10921 cgttttatct ctaccagcaa attggctcct atcgcagcca ggacggtgcg gtcaccgcgt
    10981 taattctgct actactctgt tttctgctgt ttaccgtgat tgaaaaacta ccggggcgaa
    11041 atgttaaaac tgactgatat cacctggctt tatcaccatt tgccgatgcg ttttagctta
    11101 acggtagaac gcggcgagca ggtggcaatc ctcgggccaa gcggcgcggg taaaagtacc
    11161 ctgctgaatt tgatcgccgg ttttctgacg ccagccagcg gttcgctgac tatcgacggc
    11221 gttgatcaca ccacgacgcc gccgtcacgc cgtccggtgt cgatgctgtt tcaggaaaac
    11281 aacctgttca gtcacctgac ggtcgcgcag aacatcgggc tggggctaaa tccgggattg
    11341 aaactgaacg cggcacagca ggagaaaatg cacgctatca cccgccagat ggggattgat
    11401 aatttaatgg cgcggttacc tggcgagctt tccggcggtc agcgacagcg cgtggcgtta
    11461 gcgcgttgtc tggtgcgcga acagccgatt ttattgctcg atgaaccgtt ctctgcgctc
    11521 gatccggcgt tacgtcagga gatgctgacg ctggtgagca cgagctgcca gcagcaaaaa
    11581 atgacgctat tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc cacacgctcg
    11641 gtggtggtcg ccgacgggcg catcgcctgg cagggtaaga ccaatgagtt gttgagcggt
    11701 aaggcgagtg cttcggcact attggggatt acgggttagt atgcactgtc ggatgcggcg
    11761 tgaacgcctt atccgaccta cgggtagtgg gttttgtagg cctgataaga cgcggcaagc
    11821 gtcgcatcag gcaacccgca caagactatc tcctaacccc caaccacttt acgcaaaata
    11881 tcgatataca ccggcatcaa cgggtggcga attaacacca ccagcgccac cacgccgatg
    11941 gcagaaatta acggcgtcag ccacaacaaa cgaccacggg acaaataatg actcaaacgg
    12001 tcagtcgctt taccactgcg ccataaccgc cagcacagcc agccaccaac ccacaaaaac
    12061 accgctgttg ccagcagcaa ccatttaaac tcgccactct gcattccggc tggaatatcg
    12121 atcgccgcac ccgccagaat ccccggcagg aagtaaaacg gcggccacag caggcagccg
    12181 ataatattcg gcgtaataaa ttttgccacc gggagatcca gcattcccgc caccattggc
    12241 accagcggac gcgtcgggcc aacaaagcga ccgaccagaa tggtaaacat gctgtgttga
    12301 tgcaacgcat gttcagtttt atcgagtaat gctttgtttt tcttcagaaa tgaccagcga
    12361 tgcaacggat ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc cagcaggcaa
    12421 ccgacgatcc ctgccagcca ggcgtgccag aaacttaact cgccgctgcc aatcagcgct
    12481 ccaagccccg ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc cagcgactcc
    12541 agaaaggcca ccaacaccac cgccatcaat gaatacacgg tggattgggt aataaagtgt
    12601 tccagcaatg cttgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
    12661 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc
    12721 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca
    12781 ttttttaaat actcgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
    12841 ggcgataggc atccgggtag tgctcaaaag cagcttcgcc tgactaatgc gttggtcttc
    12901 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg
    12961 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
    13021 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt
    13081 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
    13141 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg
    13201 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag
    13261 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
    13321 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
    13381 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt
    13441 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc
    13501 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg
    13561 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg
    13621 tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct cactaaccca
    13681 accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa
    13741 aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg
    13801 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg
    13861 ctttttatcg caactctcta ctgtttctcc atacccgttt ttttggatgg agtgaaacga
    13921 tggcgattgc aattggcctc gattttggca gtgattctgt gcgagctttg gcggtggact
    13981 gcgccagcgg tgaagagatc gccaccagcg tagagtggta tccccgttgg caaaaagggc
    14041 aattttgtga tgccccgaat aaccagttcc gtcatcatcc gcgtgactac attgagtcaa
    14101 tggaagcggc actgaaaacc gtgcttgcag agcttagcgt cgaacagcgc gcagctgtgg
    14161 tcgggattgg cgttgacaca accggctcga cgcccgcacc gattgatgcc gacggtaacg
    14221 tgctggcgct gcgcccggag tttgccgaaa acccgaacgc gatgttcgta ttgtggaaag
    14281 accacactgc ggttgaagaa gcggaagaga ttacccgttt gtgccacgcg ccgggcaacg
    14341 ttgactactc ccgctatatt ggcggtattt attccagcga atggttctgg gcaaaaatcc
    14401 tgcatgtgac tcgccaggac agcgccgtgg cacaatctgc cgcatcgtgg attgagctgt
    14461 gcgactgggt gccagctctg ctttccggta ccacccgccc gcaggatatt cgtcgcggac
    14521 gttgcagcgc cgggcataaa tctctgtggc acgaaagctg gggcggcctg ccgccagcca
    14581 gtttctttga tgagctggac cctatcctca atcgccattt gccttccccg ctgttcactg
    14641 acacctggac tgccgatatt ccggtgggca ccttatgccc ggaatgggcg cagcgtctcg
    14701 gcctgcctga aagcgtggtg atttccggcg gcgcgtttga ctgccatatg ggcgcagttg
    14761 gcgcaggcgc acagcctaac gcactggtaa aagttatcgg tacttccacc tgcgacattc
    14821 tgattgccga caaacagagc gttggcgagc gggcagttaa aggtatttgc ggtcaggttg
    14881 atggcagcgt ggtgcctgga tttatcggtc tggaagcagg ccaatcggcg tttggtgata
    14941 tctacgcctg gttcggtcgc gtactcagct ggccgctgga acagcttgcc gcccagcatc
    15001 cggaactgaa agcgcaaatc aacgccagcc agaaacaact gcttccggcg ctgaccgaag
    15061 catgggccaa aaatccgtct ctggatcacc tgccggtggt gctcgactgg tttaacggcc
    15121 gccgcacacc gaacgctaac caacgtctga aaggggtgat taccggtctt aacctcgcta
    15181 ccgacgctcc gctgctgttc ggcggtttga ttgctgccac cgcctttggc gcacgcgcaa
    15241 tcatggagtg ctttaccgat caggggatcg ccgtcaataa cgtgatggcg ctgggcggca
    15301 tcgcgcggaa aaaccaggtc attatgcagg cctgctgcga cgtgctgaat cgcccgctgc
    15361 aaattgttgc ctctgaccag tgctgtgcgc tcggtgcggc gatttttgct gccgtcgccg
    15421 cgaaagtgca cgcagatatc ccatcagccc agcaaaaaat ggccagtgcg gtagagaaaa
    15481 ccctgcaacc gtgcagcgag caggcacaac gctttgaaca gctttatcgc cgctatcagc
    15541 aatgggcgat gagcgccgaa caacactatc ttccaacttc cgccccggca caggctgccc
    15601 aggccgttgc gactctataa ggacacgata atgacgattt ttgataatta tgaagtgtgg
    15661 tttgtcattg gcagccagca tctgtatggc ccggaaaccc tgcgtcaggt cacccaacat
    15721 gccgagcacg ttgttaatgc gctaaatacg gaagcgaaac tgccctgcaa actggtgttg
    15781 aaaccgctgg gcaccacgcc ggatgaaatc accgctattt gccgcgacgc gaattacgac
    15841 gatcgttgcg ctggtctggt ggtgtggctg cacaccttct ccccggccaa aatgtggatc
    15901 aacggcctga ccatgctcaa caaaccgttg ctgcaattcc acacccagtt taacgcggcg
    15961 ctgccgtggg acagtatcga tatggacttt atgaacctga accagactgc tcatggcggt
    16021 cgtgagttcg gcttcattgg cgcgcgtatg cgtcagcaac atgccgtggt taccggtcac
    16081 tggcaggata aacaagccca tgagcgtatc ggctcctgga tgcgtcaggc ggtctctaaa
    16141 caggataccc gtcatctgaa agtctgccgt tttggcgata acatgcgtga agtggcggtc
    16201 accgatggcg ataaagttgc cgcacagatc aagttcggat tctccgtcaa tacctgggcg
    16261 gttggcgatc tggtgcaagt ggtgaactcc atcagcgacg gcgatgttaa cgcgctggtc
    16321 gatgagtacg aaagctgcta caccatgacg cctgccacac aaatccacgg cgaaaaacga
    16381 cagaacgtgc tggaagcggc acgtattgag ctggggatga agcgtttcct ggaacaaggt
    16441 ggcttccacg cgttcaccac cacctttgaa gatttgcacg gtctgaaaca gcttccaggt
    16501 ctggccgtac agcgtctgat gcagcagggt tacggctttg cgggcgaagg cgactggaaa
    16561 accgccgccc tgcttcgcat catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc
    16621 tttatggagg actacaccta tcacttcgag aaaggcaatg gcctggtgct cggctcccat
    16681 atgctggaag tctgcccgtc gattgccgta gaagagaaac cgatcctcga cgttcagcat
    16741 ctcggtattg gtggtaagga cgatcctgcc cgcctgatct tcaataccca aaccggccca
    16801 gcgattgtcg ccagtctgat tgatctcggc gatcgttacc gtctgctggt taactgcatc
    16861 gacacggtga aaacaccgca ctccctgcca aaactgccgg tggcgaatgc gctgtggaaa
    16921 gcgcaaccgg atctgccaac tgcttccgaa gcgtggatcc tcgctggtgg cgcgcaccat
    16981 accgttttca gccatgcgct gaacctcaac gatatgcgcc agttcgccga gatgcacgac
    17041 attgaaatca cggtgattga taacgacacc cgcctgccag cgtttaaaga cgcactgcgc
    17101 tggaacgaag tgtattacgg atttcgtcgc taagtagcca catcaggtat gtaacgcctg
    17161 atgcgacgct gacgcgtctt atctggccta cacgct
//
LOCUS       DAOFQD010000103        16795 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.103, whole genome shotgun sequence.
ACCESSION   DAOFQD010000103 DAOFQD010000000
VERSION     DAOFQD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.103"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..309
                     /gene="wcaI"
                     /locus_tag="QBF46_003479"
     CDS             <1..309
                     /gene="wcaI"
                     /locus_tag="QBF46_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDQ6675619.1"
                     /translation="DCHLVVQKRGAADAVLPSKLTNILAVGGNAVITAEAHTELGQLC
                     ETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQFINDIRG
                     "
     gene            312..1748
                     /gene="cpsB"
                     /locus_tag="QBF46_003480"
     CDS             312..1748
                     /gene="cpsB"
                     /locus_tag="QBF46_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDQ6675620.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRTAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            2029..3399
                     /gene="cpsG"
                     /locus_tag="QBF46_003481"
                     /gene_synonym="manB"
     CDS             2029..3399
                     /gene="cpsG"
                     /locus_tag="QBF46_003481"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDQ6675621.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            3454..4848
                     /gene="wcaJ"
                     /locus_tag="QBF46_003482"
     CDS             3454..4848
                     /gene="wcaJ"
                     /locus_tag="QBF46_003482"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDQ6675622.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPALCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            4850..6328
                     /gene="wzxC"
                     /locus_tag="QBF46_003483"
     CDS             4850..6328
                     /gene="wzxC"
                     /locus_tag="QBF46_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDQ6675623.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVVVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            6604..7884
                     /gene="wcaK"
                     /locus_tag="QBF46_003484"
     CDS             6604..7884
                     /gene="wcaK"
                     /locus_tag="QBF46_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDQ6675624.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNAQLNEAVSRERQAGMQMVQSVLERIGEVK"
     gene            7881..9101
                     /gene="wcaL"
                     /locus_tag="QBF46_003485"
     CDS             7881..9101
                     /gene="wcaL"
                     /locus_tag="QBF46_003485"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDQ6675625.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPQGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISNLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVIEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELTTVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            9112..10506
                     /gene="wcaM"
                     /locus_tag="QBF46_003486"
     CDS             9112..10506
                     /gene="wcaM"
                     /locus_tag="QBF46_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDQ6675626.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQIVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCENINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGRMHNITLDVRGSDCIIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQISVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            10681..11574
                     /gene="galF"
                     /locus_tag="QBF46_003487"
     CDS             10681..11574
                     /gene="galF"
                     /locus_tag="QBF46_003487"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDQ6675627.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWLELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            11946..13031
                     /gene="rfbB"
                     /locus_tag="QBF46_003488"
     CDS             11946..13031
                     /gene="rfbB"
                     /locus_tag="QBF46_003488"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDQ6675628.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNKEKLPLFIETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICNL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            13031..13930
                     /gene="rfbD"
                     /locus_tag="QBF46_003489"
     CDS             13031..13930
                     /gene="rfbD"
                     /locus_tag="QBF46_003489"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDQ6675629.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGSGDRPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEARKADIPLALNKLNAVPTTAYPTPACRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            13988..14866
                     /gene="rfbA"
                     /locus_tag="QBF46_003490"
     CDS             13988..14866
                     /gene="rfbA"
                     /locus_tag="QBF46_003490"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDQ6675630.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQLTPDGLAQAFIIGEEFIGG
                     DDCALILGDNIFYGHDLPRLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRVYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVNVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            14869..15411
                     /gene="rfbC"
                     /locus_tag="QBF46_003491"
     CDS             14869..15411
                     /gene="rfbC"
                     /locus_tag="QBF46_003491"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDQ6675631.1"
                     /translation="MNVIKTEIPDVLILEPEVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSIKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSGTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSDLAEVLYKTNQYYAPSHERNIIWNDPLLNIKWPRT
                     ALITLSDKDANGEKLELSEY"
     gene            15413..16672
                     /gene="wzx"
                     /locus_tag="QBF46_003492"
     CDS             15413..16672
                     /gene="wzx"
                     /locus_tag="QBF46_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O25 family O-antigen flippase"
                     /protein_id="HDQ6675632.1"
                     /translation="MSLLKHSIWNVAGYFIPTLIAIPAFGLIAREIGVELFGLYTLSM
                     IFIGYASIFDAGLTRAVVREIALLKNRLDDCNTIIVTSIIAVVFLGGIGGGGVFLLKD
                     HIIELLNMSPIYYVDAIKSLILLSSLIPVFLVTQILLAELEGREYFGILNIQKSLGNS
                     LIAGLPALFVLINQTLFSAIIGVAIARVICLWLSYIMSRKRITIDISFFSITVLKRLF
                     RYGGWVTISNIISPILASMDRFILSHIQGASKISFYTVPNELVTRLGIVPGSLGKAVF
                     PKLSHASSFTASYAEQKKAYILMTVIVLPLVLFIYYYAKFILTLWMGAEYAGISVEIL
                     RIMLIGYIFNCYSQISFANIQAFGKAKYTAYIHMMEFIPYLIMLYIISKECGVIGVAW
                     LWTIRVIIDFLMLFYMSYRCNNLMKKG"
BASE COUNT         4303 a         3797 c         4418 g         4277 t
ORIGIN      
        1 gattgccatc tggtggtgca aaaacgcggc gcggcagatg ccgtattgcc gtcgaaactg
       61 accaatattc tggcagtagg cggtaacgcg gtgattactg ctgaagccca cacagaactg
      121 ggacagcttt gcgaaacctt tccgggcatt gcggtttgcg tagaaccgga atcggtcgag
      181 gcgctggtgg cggggattcg tcaggcgctc ctgctgccca aacacaacac ggtggcacgt
      241 gaatatgccg aacgcacgct cgataaagag aacgtgttac gtcaatttat aaatgatatt
      301 cggggataat tatggcgcag tcgaaactct atccagttgt gatggcaggt ggctccggta
      361 gccgcttatg gccgctttcc cgcgtacttt accccaagca gtttttatgc ctgaaaggcg
      421 atcttaccat gctgcaaacc accatctgcc gcctgaacgg cgtggagtgc gaaagcccgg
      481 tggtgatttg caatgagcag caccgcttta ttgtcgcgga acagctgcgt caactgaaca
      541 aactcaccga gaacattatt ctcgaaccgg cagggcgtaa cactgcacct gccattgcgc
      601 tggcggcgct ggcggcaaaa cgtcatagcc cggagagcga cccgttaatg ctggtgctgg
      661 cggcggatca tgtgattgcc gatgaagacg cttttcgcac cgccgtgcgt aatgccatgc
      721 catatgccga agcgggcaag ctggtgacct tcggcattgt gccggatcta cctgaaaccg
      781 gttatggcta tattcgtcgc ggtgaagtgt ctgctggtga gcaggatacg gtggcctttg
      841 aagtggcgca gtttgtcgaa aaaccgaatc tggaaaccgc ccaggcctat gtggcaagcg
      901 gcgaatatta ctggaacagc ggcatgttcc tgttccgcgc cggacgctat ctcgaagaac
      961 tgaaaaaata tcgcccggat atcctcgatg cctgcgaaaa agcgatgagc gccgtcgatc
     1021 cggatctcga ttttattcgc gtggatgaag aagcgtttct cgcctgccca gaagagtcgg
     1081 tggattacgc ggtcatggaa cgtacggcag atgccgttgt ggtgccgatg gatgcgggct
     1141 ggagcgatgt cggttcctgg tcctctttat gggagatcag tgcccacacc gccgagggca
     1201 acgtttgcca cggcgatgtg attaatcaca aaactgaaaa cagctatgtg tacgccgaat
     1261 ctggcctggt caccaccgtc ggggtgaaag atttggtggt agtgcagacc aaagatgcag
     1321 tgctgattgc cgaccgtaac gcggtgcagg atgtgaaaaa agtggtcgag cagatcaaag
     1381 ccgacggtcg ccatgagcat cgggtacatc gcgaagtgta tcgtccgtgg ggcaaatatg
     1441 actctatcga cgcgggcgac cgctaccagg tgaaacgcat caccgtgaaa ccgggcgagg
     1501 gcttgtcggt acagatgcac catcaccgcg cggaacactg ggtggttgtc gcgggaacgg
     1561 caaaagtcac tattgacggt gatatcaaac tgcttggtga aaacgagtcc atttatattc
     1621 cgctgggggc gacgcactgc ctggaaaacc cggggaaaat tccgctcgat ttaattgaag
     1681 tgcgctccgg ctcttatctc gaagaggatg atgtggtgcg cttcgcggat cgctacggac
     1741 gggtgtaaac gtcgcatcag gcgctgtttg tcggatgcgg cgtgaacgcc ttatccgacc
     1801 tacggttcgg ttttgtaggc ctgataagac gcggcagcgt cgcatcaggc gctgtttgtc
     1861 ggatgcggcg tgaacgcctt atccaaccta cggttcggat ttgtaggcct gataagacgc
     1921 ggcagcgtcg catcaggcaa tgaatgcgaa accgcgatgt aaataacgac aaaaacaaaa
     1981 ttggccgctt cggtcatggc caactattgc ctgaaaaagg gtaacgatat gaaaaaatta
     2041 acctgcttta aagcctatga tattcgcgga aaactaggcg aagaactgaa tgaagatatc
     2101 gcctggcgca ttggtcgcgc ctatggcgaa tttctcaaac cgaaaaccat tgtgttaggc
     2161 ggtgatgtcc gcctcaccag cgaaacctta aaactggcgc tggcgaaagg tttgcaagat
     2221 gcgggcgttg atgtgctgga tattggcatg tccggcaccg aagagatcta tttcgccacg
     2281 ttccatctcg gcgtggatgg cggcattgaa gttaccgcca gccataatcc gatggattac
     2341 aacggtatga agctggtgcg cgaaggggct cgcccaatca gcggtgatac cggactgcgc
     2401 gatgtccagc gtctggcaga agccaatgac ttccctcctg tcgatgaaac caaacgcggt
     2461 cgctatcagc aaatcaacct gcgtgacgct tacgttgatc acctgttcgg ttatatcaac
     2521 gtcaaaaacc tcacgccgct caagctggtg atcaactccg ggaacggcgc agcgggtccg
     2581 gtggtggacg ccatcgaagc ccgctttaaa gccctcggcg cacctgtgga attaatcaaa
     2641 gtgcacaaca cgccggacgg caatttcccc aacggtattc ctaacccgct gctgccggaa
     2701 tgccgcgacg acacccgcaa tgcggtcatc aaacacggcg cggatatggg cattgccttt
     2761 gatggagatt ttgaccgctg tttcctgttt gacgaaaaag ggcagtttat cgagggctat
     2821 tacattgtcg gcctgctggc agaagcattc ctcgaaaaaa atcccggcgc gaagatcatc
     2881 cacgatccac gtctctcctg gaataccgtt gatgtggtga ctgccgcagg cggcaccccg
     2941 gtaatgtcga aaaccggaca cgcctttatt aaagaacgta tgcgcaagga agacgccatc
     3001 tacggtggcg aaatgagcgc ccaccactat ttccgtgatt tcgcttactg cgacagcggc
     3061 atgatcccgt ggctgctggt cgccgaactg gtgtgcctga aagagaaaac gctgggcgaa
     3121 ctggtacgcg accggatggc tgcgtttccg gcaagcggtg agatcaacag caaactggcg
     3181 caacccgttg aggcgattaa ccgcgtggaa cagcatttta gccgtgaggc gctggcggtg
     3241 gatcgcaccg atggcatcag catgaccttt gccgactggc gctttaacct gcgctcctcc
     3301 aacaccgaac cggtggtgcg gttgaatgtg gaatcgcgcg gcgatgtgcc gctgatggaa
     3361 gcgcgaacgc gaactctgct gacgttgctg aacgagtaat gtcggatctt cccttacccc
     3421 actgcgggta aggggctaat aacaggaaca acgatgacaa atctaaaaaa gcgcgagcga
     3481 gcgaaaacca atgcatcgtt aatctctatg gtgcaacgct tttcagatat caccatcatg
     3541 tttgccgggc tatggctggt ttgcgaagtg agcggactgt cattcctcta catgcacctg
     3601 ttggtggcgc tgattacgct ggtggtgttc cagatgctgg gcggcatcac cgatttttat
     3661 cgctcatggc gcggtgttcg ggcagcgaca gaatttgccc tgttgctaca aaactggacc
     3721 ttaagcgtga ttttcagcgc cggactggtg gcgttcaata atgatttcga cacgcaactg
     3781 aaaatctggc tggcgtggta tgggctgacc agcatcggac tggtggtttg ccgttcgtgt
     3841 attcgcattg gggctggctg gctgcgtaat catggctata acaagcgcat ggtcgcggtg
     3901 gcgggggatt tagccgccgg acaaatgctg atggagagct tccgtaatca gccgtggtta
     3961 gggtttgaag ttgtgggcgt ttaccacgac ccaaaaccgg gcggcgtttc taacgactgg
     4021 gcgggtaacc tgcaacagct ggtcgaggat gcgaaagcgg gcaagattca taacgtctat
     4081 atcgcgatgc aaatgtgcga cggcgcgcga gtgaaaaaac tggtccatca attggcggac
     4141 accacctgtt cggtgctgct gatccccgat gtctttacct tcaacattct ccattcacgt
     4201 cttgaagaga tgaacggcgt tccggtggtg ccgctttacg acacgccgct ttccggggtt
     4261 aaccgcctgc tcaaacgtgc ggaagacatt gtgctggcga cgcttatcct gctgctgatc
     4321 tccccggcgc tgtgctgtat tgcgctggcg gtgaaactca gttcacctgg gccggttatt
     4381 ttccgccaga ctcgctatgg catggatggc aaaccgatca aagtgtggaa gttccgttcg
     4441 atgaaagtga tggagaacga caaagtggtg acccaggcga cgcagaacga tccgcgcgtc
     4501 accaaagtgg ggaactttct gcgccgcacc tcgctggatg aactgccgca gtttatcaat
     4561 gtgctgaccg gggggatgtc gattgtcggt ccacgtccgc acgcagtagc gcataacgaa
     4621 cagtatcgcc agctcattga aggctacatg ctgcgccata aggtgaaacc gggcattacc
     4681 ggctgggcgc agattaatgg ctggcgcggc gaaaccgaca cgctggagaa aatggaaaaa
     4741 cgcgtcgagt tcgaccttga gtacatccgc gaatggagcg tctggttcga tatcaaaatc
     4801 gttttcctga cggtattcaa aggcttcgtt aacaaagcgg catattgata tgagcttacg
     4861 tgaaaaaacc atcagcggcg cgaagtggtc ggcgattgcc acggtgatca tcatcggcct
     4921 cgggctggtg cagatgaccg tgctggcgcg gattatcgac aaccaccagt tcggtttgct
     4981 taccgtgtcg ctggtgatca tcgcgctggc agatacgctt tctgacttcg gtattgctaa
     5041 ctcgattatt cagcgaaaag aaatcagcca ccttgaactc accacgctgt actggctgaa
     5101 cgttgggctg gggcttgtgg tgtgtgtggt ggtgtttttg ttgagtgatc tcatcggcga
     5161 cgtgctgaat aacccggacc tggcaccgtt gattaaaaca ttgtcgctgg cgtttgtggt
     5221 gatcccccac gggcaacagt tccgcgcgtt gatgcaaaaa gagctggagt tcaacaaaat
     5281 cggcatgatc gaaaccagcg cggtgctggc gggcttcact tttacagtgg ttagcgccca
     5341 tttctggccg ctggcgatga ccgcgatcct cggttatctg gtcaatagtg cggtgagaac
     5401 gctgctattt ggctactttg gccggaaaat ttatcgcccc ggtctgcatt tctcgctggc
     5461 gtctgttgcg ccgaacttac gctttggtgc ctggctgacg gcggacagca tcatcaacta
     5521 tctcaacacc aacctttcaa cgctggtgct ggcgcgtatt ctcggcgcgg gcgtggcagg
     5581 gggatacaac ctggcgtaca acgtggccgt tgtgccgccg atgaagctga acccgatcat
     5641 cacccgcgtg ttgtttccgg cattcgccaa aattcaggac gataccgaaa agctgcgcgt
     5701 taacttctac aagctgttgt cggtagtggg gattatcaac tttccggcgc tactggggct
     5761 gatggtggtg tcgaataact ttgtaccgct ggtctttggt gagaagtgga acagcattat
     5821 tccggtgctg caattgctgt gtgtggtggg gctactgcgc tccgtgggta acccgattgg
     5881 ttcgctgctg atggcgaaag cacgggtgga catcagcttt aaattcaacg tattcaaaac
     5941 atttctgttt attccggcga ttgttattgg tgggcagatg gcgggcgcaa tcggcgtcac
     6001 gcttggcttc ctgctggtgc agattatcaa caccattctg agctatttcg tgatgattaa
     6061 accggtgctc ggttccagtt atcgccagta catcctgagt ttgtggctgc cgttttatct
     6121 ctcgctgccg acgctggtgg tcagttatgt gctgggcatt gtgctgaaag ggcaactggc
     6181 gctggggatg ctgctggcgg tgcaaatagc cgcgggcgtg ctggcgtttg tagtgatgat
     6241 tgtgctgtcg cgccatccgc tggtggtgga agtgaagcgt cagttttgtc gcagcgaaaa
     6301 aatgaaaatg cttttacggg cggggtgagt tgttgttttc ggttccgaca acggttgccg
     6361 agtgcagcgt aaaacgcctt atccatccta cggttctgtg ctcgggacct gtaggcctga
     6421 taagacgcgt cagcgtcgca tcaggcactg aatgccggat gcggcgtgaa cgccttatcc
     6481 ggcctacggt tctgtgctcg ggtcttgtag gcctgataag acgcgccagc gtcgcatcag
     6541 gcaaaaaacg gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa
     6601 aaaatgaaat tacttatttt gggcaaccac acttgcggca atcgtggcga cagcgccatc
     6661 ttgcgcggct tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg
     6721 agccgctatc cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg
     6781 ttccttcaaa tgaaacaaca caacagcgcg gcgggcgttg tcgggcgcgt taaaaaagtc
     6841 ctccgtcgcc gctatcaaca ccaggtattg ctctcgcgcg tcaccgacac tggcaagctg
     6901 cgcaatattg ccatcgccca gggattcact gacttcgtgc gcctgctgtc aggttacgat
     6961 gccattatcc aggtcggcgg atcgtttttt gtcgatctct atggcgtgcc gcagtttgaa
     7021 catgcacttt gcacgtttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggt
     7081 cccttccagg atgagcaatt taaccaactg gcgaactacg tttttggtca ctgtgacgcg
     7141 ctgatcctgc gcgaatcggt cagccttgat ctgatgaaac gcagcaatat caccaccgca
     7201 aaagtggaac atggcgtcga taccgcgtgg ctggtcgatc accacaccga agatttcacc
     7261 gccagctatg ccgttcaaca ctggctggac gttgccgcac aacagaaaac ggtagcaatt
     7321 accctgcgcg aactggcacc gttcgacaaa cgtctcggca ccactcagca agcgtatgaa
     7381 aaagcctttg ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc
     7441 tccacctgta cgggcatcga cagctataac aaagacgacc gcatggtggc gctcaatctt
     7501 cgccagcaca tcagcgatcc tgcccgttac catgtggtga tggatgaact caacgatctg
     7561 gaaatgggca aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactctgcc
     7621 attatctcga tgaatttctc cactccggca attgccatca actacgaaca taaatccgcc
     7681 gggattatgc agcagttggg actaccagag atggcgattg atatccgtca tttactggac
     7741 ggcagcctgc aagcgatggt tgcggatacc ttaggccagc ttccggcgct gaatgcacaa
     7801 cttaacgaag ccgtcagtcg cgaacgtcag gcaggaatgc agatggtgca atctgtgctt
     7861 gaacgcatcg gggaggtgaa atgaaggtcg gcttcttttt actgaaattt ccgctgtcgt
     7921 cggaaacctt cgtcctcaat caaattaccg cgtttattga tatgggcttt gaggtggaga
     7981 ttgtcgcgct gcaaaaaggc gacacacaaa acacccacgc ggcatggaca aaatataacc
     8041 ttgccgccag aacccgctgg ttacaggacg agccacaagg caaagtggcg aaactgcgcc
     8101 accgcgccag ccagacgttg cgcggtattc atcgtaaaaa tacctggcag gcgcttaacc
     8161 tcaaacgcta tggtgctgag tcgcggaacc tgattttgtc tgccatttgc ggccaggtcg
     8221 caacaccgtt tcatgccgat gtgtttatcg ctcattttgg ccctgctggg gtaaccgcgg
     8281 caaaactacg cgaactgggt gtcattcgcg gcaaaattgc cactatcttc cacggtattg
     8341 atatctccag tcgggaagtg ctcaaccact acactcccga atatcaacaa ttgtttcgcc
     8401 gtggcgacct gatgttacca ataagcaatc tgtgggccgg aaggctgcaa aaaatgggct
     8461 gcccgaggga aaaaatcgcc gtatcgcgca tgggcgtgga catgacgcgt tttagcccgc
     8521 gtccggtgaa agcgcccgca acgccgctgg aaatcatctc cgtcgcacgc ttaaccgaga
     8581 aaaaaggcct gcatgtggcg attgaagcct gccgccagtt gaaagagcag ggcgtggcat
     8641 ttcgctatcg catcctcggc attggcccgt gggaacgacg cctgcgcacc ctcatcgaac
     8701 aatatcaact ggaagatgtg atagagatgc cgggctttaa accgagccat gaagtgaaag
     8761 cgatgctcga tgacgcggat gtcttcctgt tgccatcggt tacaggtgcg gatggcgata
     8821 tggaaggtat tccggtggcg ctaatggaag cgatggcggt tggcattccg gttgtttcta
     8881 ctctgcacag cggaataccg gaactggtgg aggccgataa atccggttgg ctggtgcctg
     8941 agaacgatgc tcgcgcactg gcgcaacgac tggcggcgtt tagccaactg gacaccgacg
     9001 aacttactac ggttgtcaaa cgtgcgcgcg aaaaagtcga acacgatttt aaccagcagg
     9061 tgatcaatcg agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt
     9121 aaaaaactct cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc
     9181 cctttcgctc gcgcgcttcc cgcccgacaa atcgttaaca ttaacgacta caacccacac
     9241 gactggatcg cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc
     9301 ggattcgttt gtgaaaatat caacaccggc atcttcattc ctcctggcaa aacgttgcac
     9361 atccttggaa gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag
     9421 gtgacagggg agaagggcgg ccgtatgcat aacatcaccc tggatgtgcg cggctctgac
     9481 tgcatcatca aagggttggc gatgagcggt tttggcccgg taacgcagat ttatatcggc
     9541 ggcaaaaata aacgcgtcat gcgcaacttg accatcgata acctgaccgt cagccacgct
     9601 aattacgcca ttttacgcca gggatttcat aaccagatta tcggtgccaa catcaccaat
     9661 tgtaagttca gcgacttaca gggcgacgcc attgaatgga atgtagcaat caatgatcgc
     9721 gacattttga tatccgacca tgtcatcgag cgcatcaact gtactaacgg caaaatcaac
     9781 tggggtattg gcataggact ggcaggaagt acttacgata ataattatcc ggaaaaccag
     9841 gcagtgaaaa actttgtcgt tgcgaatatc acgggatcag attgtcggca actgatccat
     9901 gttgaaaatg gtaaacattt tgttatccgt aatatcaaag ctcgtaatat cacgccggat
     9961 ttcagtaaga aagcaggtat tgataacgcc acagttgcta tttatggttg tgacaacttt
    10021 gtgattgata atattgaaat gattaatagt gccgggatgt taatcggcta tggggtaatt
    10081 aaaggcaaat atctctcgat accgcagaat ttccgagtga atgatattca actggataat
    10141 actcatcttg cttataaatt gcgcggcatc caaatctccg ctgggaatgc tgtctccttt
    10201 gtggcgctga ctaacattga gatgaagcgt gcgtcgctgg agttacacaa caaaccgcaa
    10261 catcttttta tgcgaaatat caatgtgatg caggaatcct cagttggacc cgcattgagc
    10321 atgaactttg acatgcgcaa agacgttcgc ggcgtgttta tggcgaaaaa agaaacactg
    10381 ctgtctcttg caaatgttca tgcggtgaat gaaaaaggac aaatctccgt cgatatcgac
    10441 aggattaatc accatattgt taatgtggag aagattaact ttagattgcc agaacggagg
    10501 gagtagattt gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca
    10561 atccacaacg aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat
    10621 gctggcgaaa aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata
    10681 atgacgaatt taaaagcagt tattcctgta gcgggtcttg ggatgcatat gttgcctgcc
    10741 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatc
    10801 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag
    10861 aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctctcct tgaacagcgc
    10921 gtgaagcgtc aactgctggc ggaagtgcaa tctatctgcc cgccgggcgt gactattatg
    10981 aacgtgcgtc agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc
    11041 attggtgaca acccgtttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc
    11101 gacccgctac gttacaacct tgctgccatg attgcgcgct tcaacgaaac gggccgcagc
    11161 caggtgctgg caaaacgtat gccgggtgac ctctctgaat actcggtcat ccagaccaaa
    11221 gaaccgctgg atcgcgaagg taaagtcagc cgcattgttg aatttatcga aaaaccggat
    11281 cagccgcaga cgctggactc agacatcatg gccgttggtc gctatgtgct ttctgccgat
    11341 atttggctgg aacttgaacg cacgcagcct ggtgcatggg gacgtattca gctgactgat
    11401 gccattgctg aactggcgaa aaaacagtcc gttgatgcca tgctgatgac cggcgacagc
    11461 tacgactgcg gtaaaaaaat gggctatatg caggcgtttg tgaagtatgg actgcgtaac
    11521 ctaaaagaag gggcgaagtt ccgtaaaggg attgagaagc tgttaagcga ataatgaaaa
    11581 tctgaccgga tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcgaaa
    11641 gtaatttgct gcgaatattc ctgccgttgt tttatataaa caatcagaat aacaacgagt
    11701 tagcaatagg attttagtca aagttttcca ggattttcct tgtttccaga gcggattggt
    11761 aagacaatta gcgtttgaat ttttcgggtt tagcgcgagt gggtaacgct cgtcacatcg
    11821 tagacatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gcgttaatac
    11881 ctctattaat caaactaaga gccgctaatt tcacagcatg ctctgaagta atatggaata
    11941 aaaaagtgaa gatacttgtt actggtggcg caggatttat tggttctgct gtagttcgtc
    12001 acattataaa taatacgcag gatagtgttg ttaatgtcga taaattaacg tacgccggaa
    12061 acctggaatc acttgctgat gtttctgatt ctgaacgcta tgtttttgaa catgcggata
    12121 tttgcgatgc tgctgcaatg gcgcggattt ttgctcagca tcagccggat gcagtgatgc
    12181 acctggctgc tgaaagccat gtggatcgtt ctatcactgg ccctgcggca tttattgaaa
    12241 ccaatattgt tggtacttat gtccttttag aagctgctcg caattactgg tctgctcttg
    12301 atagcgacaa gaaaaatagc ttccgttttc atcatatttc tactgacgaa gtctatggtg
    12361 atttgcctca tcctgacgaa gtaaataata aagaaaaatt acccttattt attgagacaa
    12421 cagcttacgc accaagcagc ccttattccg catccaaagc atccagcgat catttagtcc
    12481 gcgcgtggaa acgtacctat ggtttaccga ccattgtgac taactgttcg aataactacg
    12541 gtccttatca ctttccggaa aaattgattc cactagtaat tcttaatgct ctggaaggta
    12601 aggcattacc tatttatggc aaaggggatc aaattcgtga ttggctgtat gttgaagatc
    12661 atgcgcgtgc attatatacc gtcgtaaccg aaggtaaagc gggtgaaact tataacattg
    12721 gtgggcacaa cgaaaagaaa aacattgatg tagtgctcac tatttgtaat ttgctggatg
    12781 agattgtacc gaaagagaaa tcttatcgtg agcaaatcac ttatgttgcc gatcgtccgg
    12841 gacacgatcg ccgttatgcc attgatgctg agaagattgg tcgcgaattg ggatggaaac
    12901 cacaggaaac gtttgagagc gggattcgga agacagtgga atggtacctg tccaatacaa
    12961 aatgggttga taatgtaaaa agtggtgcct atcaatcgtg gattgaacag aactatgagg
    13021 gccgccagta atgaatatcc tcctttttgg caaaacaggg caggtaggtt gggaactaca
    13081 gcgtgctctg gcacctttgg gtaatttgat tgctcttgat gttcactcca ctgattactg
    13141 tggtgatttt agtaatcctg aaggtgtagc tgaaaccgta agaagcattc ggcctgatat
    13201 tattgtcaac gcagccgctc acaccgcagt agacaaagca gaatcagaac cggagtttgc
    13261 acaattactt aacgcaacaa gtgtcgaagc gattgcgaaa gcagcaaatg aagttggagc
    13321 ttgggttata cactactcca ccgattatgt tttccctggc agtggtgaca ggccatggct
    13381 ggaaacggat gcaacagcgc cgctaaatgt ttacggtgaa actaagctag ctggggaaaa
    13441 agcgttacaa gaacattgcg caaagcatct tattttccgt accagctggg tatacgctgg
    13501 taaaggaaat aattttgcca aaacgatgtt gcgtctggca aaagaacgcg aagagttggc
    13561 tgtgataaac gatcaatttg gcgcaccaac gggtgctgag cttctggccg attgtacagc
    13621 acatgccatt cgtgtcgcac tgaataaacc ggatgtcgca ggcttgtacc atctggtagc
    13681 tagtggtacc acgacctggt acgattatgc tgcgctggtt tttgaagagg cgcgcaaagc
    13741 agatattccc ctcgcactca acaaactcaa tgcggtacca acgacagcct atcctacacc
    13801 agcttgtcgt ccacataact ctcgacttaa tacagaaaaa tttcagcaga attttgcgct
    13861 tgtcttgcct gactggcagg ttggtgtgaa acgcatgctc aacgaattat ttacgactac
    13921 agcaatttaa tagtttttgc atcttgttcg tgatgatgga gcaagatgaa ttaaaaggaa
    13981 tgatgaaatg aaaacgcgta agggtattat tttagcgggt ggttctggta ctcgtcttta
    14041 tcctgtgact atggcagtca gtaaacagct attaccgatt tatgataaac cgatgatcta
    14101 ttacccgctc tctacactga tgttggcggg tattcgcgat attttgatta ttagtacgcc
    14161 acaggatact cctcgttttc aacaactgct gggtgacggt agccagtggg ggctaaatct
    14221 tcagtacaaa gtgcaactga ctccagatgg gcttgcgcag gcatttatta tcggtgaaga
    14281 gtttattggt ggtgatgatt gtgctttgat tcttggtgat aatatctttt acggtcacga
    14341 tctgccgagg ttaatggatg ccgctgttaa caaagaaagt ggtgcaacgg tatttgccta
    14401 tcacgtaaat gatcctgaac gctatggtgt cgttgagttt gataaaaacg gtactgcaat
    14461 cagcttggaa gaaaaaccgt tacaaccaaa aagtaattat gcggtaaccg ggctttattt
    14521 ctatgataac gacgttgtgg aaatggcgaa aaaccttaag ccttctgccc gtggtgaact
    14581 ggaaattacc gatattaacc gtgtctatat ggaacagggg cgtttatctg ttgccatgat
    14641 gggccgtggt tatgcatggt tagacacggg gacacatcaa agtctgattg aagcaagtaa
    14701 cttcattgca acaattgaag agcgacaagg tttaaaggta tcttgcccgg aagaaattgc
    14761 ttatcgtaaa ggctttattg acgctgagca ggttaatgta ttagcagaac cactaaagaa
    14821 aaatgcttat ggtcagtatc tgctaaaaat gattaaaggt tactaaaaat gaatgtaatt
    14881 aaaactgaaa ttcctgatgt attaattttg gaaccggaag tttttggtga tgagcgcggt
    14941 ttttttatgg aaagctttaa tcagaaagtt ttcgaagagg ctgtagggcg taaggttgaa
    15001 tttgttcagg ataaccattc taaatcaatt aagggtgtat tacgcggatt gcactatcag
    15061 ctggaacctt atgctcaagg taaattagtt cgttgtgtgg tcggtgaggt ttttgatgta
    15121 gcagttgata ttcgtaaatc gtcaggtaca tttgggaaat gggttggggt gaatttgtct
    15181 gctgagaaca agcgtcagtt gtggatacct gaaggatttg ctcatggatt tttggtactt
    15241 agtgatttag cagaagtttt atataaaacg aatcaatatt atgctccatc acatgaacga
    15301 aatattatct ggaatgaccc cttgcttaat attaaatggc caaggacagc actgattact
    15361 ctgtctgata aggatgcaaa tggggaaaaa ttagaactaa gtgagtattg aaatgtctct
    15421 cttaaaacat agtatatgga atgttgcggg ctactttata ccaacattaa ttgcaatccc
    15481 agcgtttgga ttaattgcaa gggaaattgg tgtagaacta tttggtttgt atactttatc
    15541 aatgattttt atagggtatg ctagtatatt tgatgctggg ttaacaagag ctgtagtacg
    15601 cgaaatagca ttactaaaaa acagactgga cgattgtaat acgataatag taacttctat
    15661 tattgctgtg gtatttttag ggggtatcgg aggcggggga gtgtttctgc ttaaagacca
    15721 tattattgaa ctgttaaata tgtcaccaat atactacgtc gatgcgataa agtctctaat
    15781 attattatca tctctgatac ctgtattctt agtcacgcaa atactattag cagagcttga
    15841 ggggcgggaa tattttggaa tcctaaatat acaaaaaagt ttagggaatt ctttaattgc
    15901 agggttgcct gcattatttg ttttaattaa tcaaacactt ttttctgcaa ttattggtgt
    15961 agctattgca agagttatat gcttgtggtt aagctacatt atgagcagga aaagaataac
    16021 tatcgatatt tcattttttt caataactgt tttaaaacgg ttatttagat atggcgggtg
    16081 ggtaactata agtaacataa tatctcctat attagcgagt atggatagat ttattctatc
    16141 tcatatccag ggagcatcaa aaatatcatt ctatacagtc cctaatgagc tggtaactag
    16201 gcttggaata gttccaggct ctcttgggaa agctgttttt ccaaaattaa gccatgcaag
    16261 tagttttaca gcgtcatatg cagaacaaaa aaaagcgtat atattaatga ctgtcattgt
    16321 attgcctttg gttttattta tatattacta cgcaaagttt attttaacat tgtggatggg
    16381 ggctgagtat gcagggattt cagtcgaaat attacggatt atgcttatag ggtatatctt
    16441 taactgttat tcacaaatct cttttgccaa tatacaggca tttggaaaag caaaatacac
    16501 tgcatacatc catatgatgg aatttattcc ttatttgata atgttatata taatttcaaa
    16561 ggaatgtggg gttattggtg tggcgtggtt atggacaatt cgagtgataa ttgatttttt
    16621 gatgcttttt tatatgagtt atcgttgtaa taatcttatg aaaaaagggt agcctgatga
    16681 tatatattgt agtgttaaat tggaatgggg ctatagatac cattaattgt gttaaaagtt
    16741 taatgaattt aaattatgat gattataaaa ttatcgttgt tgataactgt tctac
//
LOCUS       DAOFQD010000104        16758 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.104, whole genome shotgun sequence.
ACCESSION   DAOFQD010000104 DAOFQD010000000
VERSION     DAOFQD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.104"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(451..827)
                     /gene="rnpB"
                     /locus_tag="QBF46_003493"
     ncRNA           complement(451..827)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="QBF46_003493"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(860..2005)
                     /gene="garK"
                     /locus_tag="QBF46_003494"
     CDS             complement(860..2005)
                     /gene="garK"
                     /locus_tag="QBF46_003494"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDQ6675633.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2102..2992)
                     /gene="garR"
                     /locus_tag="QBF46_003495"
     CDS             complement(2102..2992)
                     /gene="garR"
                     /locus_tag="QBF46_003495"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDQ6675634.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3022..3792)
                     /gene="garL"
                     /locus_tag="QBF46_003496"
     CDS             complement(3022..3792)
                     /gene="garL"
                     /locus_tag="QBF46_003496"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDQ6675635.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFIET
                     KEEAELAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3808..5142)
                     /gene="garP"
                     /locus_tag="QBF46_003497"
     CDS             complement(3808..5142)
                     /gene="garP"
                     /locus_tag="QBF46_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDQ6675636.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5517..7088
                     /gene="garD"
                     /locus_tag="QBF46_003498"
     CDS             5517..7088
                     /gene="garD"
                     /locus_tag="QBF46_003498"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDQ6675637.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7237..7572
                     /gene="prlF"
                     /locus_tag="QBF46_003499"
     CDS             7237..7572
                     /gene="prlF"
                     /locus_tag="QBF46_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDQ6675638.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7572..8036
                     /gene="yhaV"
                     /locus_tag="QBF46_003500"
     CDS             7572..8036
                     /gene="yhaV"
                     /locus_tag="QBF46_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDQ6675639.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFDAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8091..8900)
                     /gene="agaR"
                     /locus_tag="QBF46_003501"
     CDS             complement(8091..8900)
                     /gene="agaR"
                     /locus_tag="QBF46_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDQ6675640.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9149..10429
                     /gene="kbaZ"
                     /locus_tag="QBF46_003502"
     CDS             9149..10429
                     /gene="kbaZ"
                     /locus_tag="QBF46_003502"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDQ6675641.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10452..10925
                     /gene="agaV"
                     /locus_tag="QBF46_003503"
     CDS             10452..10925
                     /gene="agaV"
                     /locus_tag="QBF46_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDQ6675642.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10936..11715
                     /locus_tag="QBF46_003504"
     CDS             10936..11715
                     /locus_tag="QBF46_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDQ6675643.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11705..12583
                     /locus_tag="QBF46_003505"
     CDS             11705..12583
                     /locus_tag="QBF46_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDQ6675644.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQNIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12601..13035
                     /locus_tag="QBF46_003506"
     CDS             12601..13035
                     /locus_tag="QBF46_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDQ6675645.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13032..14165
                     /gene="nagA"
                     /locus_tag="QBF46_003507"
     CDS             13032..14165
                     /gene="nagA"
                     /locus_tag="QBF46_003507"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDQ6675646.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14516..15670
                     /locus_tag="QBF46_003508"
     CDS             14516..15670
                     /locus_tag="QBF46_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDQ6675647.1"
                     /translation="MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15683..16543
                     /gene="kbaY"
                     /locus_tag="QBF46_003509"
     CDS             15683..16543
                     /gene="kbaY"
                     /locus_tag="QBF46_003509"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDQ6675648.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         4045 a         4434 c         4285 g         3994 t
ORIGIN      
        1 cctgtgcacc aaaagcaaaa acttatcgat gcgggtttga aactgatggc aaccgcaaaa
       61 atgcctgatg cgctacgctt atcaggccta cgccatctct gcaatatatt gaatttgcgt
      121 gcttttgtag gcaggataag gcgttcacac cgcatccggc atgaataaag cgcactcaac
      181 aatctgaaac ccgccggaac ggtttattac gtacatcagg tgaaactgac cgataagccg
      241 ctttcttttg ggtatagcgt cgtggacagt cattcatctt tctgcccctc caaaagcaaa
      301 aacccgccgg tttacgctgg cgggttttcg ttaaatcagg tgaaactgac cgataagccg
      361 ctttcttttg ggtatagtgt cgtggacagt cattcatctt tctgcccctc caaaagcaaa
      421 aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag ccgggttctg
      481 tcgtggacag tcattcatct aggccagcaa tcgctcactg gctcaagcag cctacccggg
      541 ttcagtacgg gccgtacctt atgaacccct atttggcctt gctccgggtg gagtttaccg
      601 tgccacggac tgttaccagc cgcgcggtgc gctcttaccg caccctttca cccttacctg
      661 atcccgcttg cgcgggccat cggcggtttg ctctctgttg cactggtcgt gggtttcccc
      721 cccaggcgtt acctggcacc ctgccctatg gagcccggac tttcctcccc tccgcccgtc
      781 tcccccgaag aggacgacga cgaagcggcg actgtctggt cagcttcggc gcgcagtata
      841 gagggtttgc gcgcccttgt caccccgcgt tgcgcattcc aatcgccagt gtcgcggcga
      901 tattacgtga agcacggcag atattgtcat aagccccgcg gaatgcttcg tccaacgtac
      961 ctatgctggt caatacgctg aagaccgcat caatgccatg ctgatgtaca acgccaacat
     1021 catcggtcag gctacccgca atgccaatca ccggtttatg gtacttcttc gccacgtttg
     1081 cgacaccaat cggtaccttc ccgtgaatac tctggctgtc aatacgccct tcaccggtga
     1141 tcaccagcgt acaatcgtga atatgttcct ccagattcag cgccgtagtg acgatttcaa
     1201 taccactttt cagttccgca ccaagaaacg ccattagcgc cgcgcccata ccacccgcag
     1261 ctcctgcacc ggggacatct ttcacatcaa catgcagcgc ttttttaatg acctcggcat
     1321 agtgagagag gttattgtcc agctcaacaa tcatcgcttc actggctccc ttttgtgggc
     1381 caaagatgcg cgatgcgccg ttatcgccca ccagcggatt ggtgacatca caagcgacgc
     1441 gaatgacgca atcttttaag cgcggatcga ggccggaaat atcaatatca ttcagagtat
     1501 taagactacc gccgccaaaa ccaatttcat tgccgttggc gtcgcataat ttcgccccca
     1561 gcgcctgtac catgcctgcg ccgccatcat ttgtagcgct gccgccaatg ccgataataa
     1621 tgtttgtcgc accgctctcc agcgcctgca ggattaactc gcctgtgccg cgtgaagtgg
     1681 tcacgagtgg atcgcgtttt tccgcaggta ccagctccag cccactggcc gccgccattt
     1741 caataaacgc ggttttgcca tcgccggaga tcccccaact ggcattcact ttctcgccca
     1801 gcggccctgt aacccaggcg tgacgttcag ccccctgggt ggctgcaatc atcgcttcca
     1861 ccgttccttc gccaccgtcg gcaaccggaa cagaaacgta ctgtgcatca ggaaaaattt
     1921 cccgaaatcc tttttctatc gcctgcgcaa cctcgctggc agataaactt tctttataag
     1981 agtctggggc gattacgatt ttcataccta tgcctgttac cacatgacgc cggagggcgt
     2041 ttctcttatt cggcctggat tccaggcccg gattgcaata cgccatccgg gcacgacgtc
     2101 attaacgagt aacttcgact ttcgccagtt tttcgtagta gcacgccagg gcgctatgat
     2161 ccgccgttcc taaaccatct gctcgcagtg cctgcatcat ctccataacc gcagctgtga
     2221 gcggcagttg tgcaccgacg ccgtgagaag tatccagcgc attcgccaga tccttaatat
     2281 gcagatcaat acggaagccc ggcttgaagt tgcggtccat caccatcggc gctttggcat
     2341 ccagcacggt actgcccgcc agtccaccgc gaattgcctg ataaaccagg tccgggttaa
     2401 cgcccgcttt agttgccagc gttaacgctt ctgacatcgc ggcaatattc agcgccacaa
     2461 tgacctgatt tgccagtttg gtgacgttac ctgcaccgat ttccccggta tgcaccacgg
     2521 aacccgccat cgctttcatc aaatcatagt atttgtcgaa aatagccttg tcgccgccca
     2581 ccatcactga cagcgtaccg tcgatggctt tcggttcacc gccgctcacc ggagcatcca
     2641 gcatatcaat gcctttcgct ttcagcgctt cgctgatttc acggcttgcc agcggtgcga
     2701 tagaactcat atcgatcaat accgtacctg gcttcgcgcc ttcaataatg ccattctcac
     2761 ccagcgccac ctctttcaca tgaggggagt ttggcagcat ggttatgatg acgtcgcact
     2821 gttcagcgat cgctttagcc gtagacgctg tttctgcacc tgcagcaatc acgtcagcaa
     2881 tagcttctgg gttacggtca gcaaccacca gcgagtaacc tgctttcaga aggtttttac
     2941 tcattggttt acccataatc cccaggccaa taaaaccaac tttcatagtc atatcaatca
     3001 tctctcttgt tgcggtggtg gttatttttt aaaggtatca gccagtttct gagtggcaga
     3061 gcggaagacg ccgagatcgc tgccgacagc cacaaacgtc gcgccccatt ccagataacg
     3121 acgcgcatcg gcttcgaccg gcgcgaggat accgctgggt ttgccgtgcg cgctggcacg
     3181 gttaaaaatg tgctgaattg ctttttgtac atccgggtgt gatgcattgc cgagatggcc
     3241 taatgccgcg gccagatcgc tggggccgac gaagatgccg tctacgcctt cggtagcggc
     3301 aatggcatcg acgttatcta cgccctgctg actttctatc tggaccagaa tagtgatgtt
     3361 cttgttcgac tgagcgaaat aatccgccac ggtgccaaac atattggcgc ggtgagaaac
     3421 ggagacgccg cgaatgcctt ccggtgggta acgggttgat gccaccgcca gctctgcttc
     3481 ctcttttgtt tctataaaag gaatcaggaa gttatagaaa ccgatatcca gaagacgctt
     3541 aataattacc ggctcgttgg tcggcactcg cactactggc gcgctggcgc tgcctttcaa
     3601 ggccattaac tgcggaataa acgtggagat atcgtttggc gcatgttcgc catccagcac
     3661 cagccagtca aacccagcca aaccaagaac ttcagtgcta atcgggttag agagtgctga
     3721 ccagcaacca atttgtacct gtttcgcagc cagtgcggct ttgaatttat tcgggaaaac
     3781 atcgttattc atcgcttata cctttgctta tttctgcaat tccatacgtt taatgtcgcc
     3841 aactacgaag aggtagcaga ccatcgccat cagcgctgaa catcccacga aaaccagtgc
     3901 tgcattgaag gagtgcagtt cacttaccag gtagccaatc accagtggag tgacaatgga
     3961 ggcaacattg ccaaagacgt taaagacgcc gccgcagagg ccaacaatct ctttcggcgc
     4021 ggtgtcagaa atcaccggcc agcccagcgc accaaatcct ttgccaaaga aagccagcgc
     4081 catcagcatg accaccagcg tggtgttgtt ggtgtagtta cataagatga tggtggaagc
     4141 cagcaacatt cccagcacaa tcggtagctt acgtgccagg gtcagggata aaccgcgttt
     4201 gatcagataa tccgagaaga cacctcccag cacgccgccc gcaaaaccac acagtgctgg
     4261 aatcgaggcg accagaccca ctttcagaat cgacatgcct ttttcctgca ccagataaat
     4321 cgggaaccag gtgaggaaga accaggtgat ggtgttgata aaatattgtc cgaaaaatac
     4381 gcccagcatc atgcggttag agagcaattg cttgatgtaa tgcagtttgg gtccgcttgc
     4441 tgccgcactg cccggctttt tgtggtccat atcgaccacc gcgccatttt cagagataaa
     4501 cttcagctct tccgcagaca tacgtgggtg atctgtcggg ttatgaatca acttgatcca
     4561 cagcgccgtc agcacaaaac caatcacccc cataacggta aagacgtgct cccagcccca
     4621 ggcgaaagtc agccagccaa gcagcggcga aaagagcgcc agcgagaaat attgcgccga
     4681 gttaaagatg gcggaggcag taccacgttc tttcgtcggg aaccaggcgg cgacaattcg
     4741 ggcgttcgcc gggaatgatg gcgcttccga gaagccgagc ataaagcgca taaagaacat
     4801 ggagatccct gcccaggcca gcgggaacat atcaacaaag ccttgcagga aggtgaatag
     4861 cgaccagaaa aagaggctgt aggtgtaaac ttttttcgag ccaaacttat caagcagcca
     4921 gccgccgggg atttgcatca gcaagtaggc ccagccaaaa gcggagaaga tgtaacccat
     4981 cgaaaccgca cttaactgca actcttttgc cacttcggta ccagcaatag acagcgttgc
     5041 acgatcggcg tagttaacgg cggtaacaat aaaaataatc agtaatatta aatagcgggt
     5101 atgcacgcct ttcttttttt cgtcaacggt gtccagaatc attttattta cctcgggtac
     5161 ttatgctgat ttttattatt atagggaagg tgttatttat gagtttcatt tatgccgtaa
     5221 cgacaatgaa ctcgggaatt agtataagca gcgcgagaat aataatcatt gtgcaaatgc
     5281 taatttaatt aatactattt aaatattatt ttgagcatat gcacataagg ttgcgcgcta
     5341 aagcacagat ttgcgcttta ccttaccggg cggcactgca atccctgaaa tgattgacat
     5401 tgatcacatt tctgcgttta aactcctgac attcttattt cacccaatga agtcatttat
     5461 ttttaaatga gaccaggtcc tcattttaat aacccctggc tggagaatat tgcacaatgg
     5521 ccaacatcga aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt cacgacacag
     5581 ataatgtggc aattattgtt aatgataatg gcctgaaagc aggaacgcgt tttccggatg
     5641 ggctggaatt aattgaacat attccccagg ggcataaagt cgcattgctg gacattccgg
     5701 ctaatggtga aattattcgt tatggcgaag tgattggtta cgccgtgcgt gcaatcccac
     5761 gcggaagctg gatcgacgaa tcaatggttg tactaccgga agcgccgccg ttacacacgc
     5821 tgccactggc aaccaaagtc ccggaaccct taccgccgct ggaaggatac acctttgagg
     5881 gctatcgcaa tgccgatggc agcgtgggca ccaaaaacct gctcggtatc accaccagcg
     5941 tccactgtgt ggcaggcgtg gtggactatg tagtaaaaat cattgaacgc gatctgctac
     6001 cgaaataccc gaacgtcgat ggcgtggtgg ggctgaatca tttgtacggt tgtggcgtgg
     6061 cgattaacgc accggcggca gttgtaccta tccgtaccat tcacaatatt tcgctgaatc
     6121 ctaactttgg cggcgaagta atggtgattg gcctgggttg tgaaaagttg cagcctgagc
     6181 gcctgctgac tggaacggat gatgtgcaag ctattccagt agaaagcgcc agcattgtca
     6241 gtttgcagga tgaaaagcat gtcggttttc agtccatggt cgaggatatt ttgcagatcg
     6301 ccgaacgcca tctacaaaaa ctgaatcaac ggcagcgaga aacctgcccg gcttcagaac
     6361 tggtcgttgg tatgcagtgc ggtggcagcg atgcgttttc tggtgtaacg gcaaacccgg
     6421 cggttggcta tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg ttttcagaag
     6481 taacggaagt gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac gaagaggtcg
     6541 gcaaacggct gctggaggag atggagtggt acgataacta tctcaatatg ggaaaaaccg
     6601 accgcagcgc caacccttcg ccgggcaaca agaaaggcgg tctggcaaac gtggtagaga
     6661 aggcactcgg ctccattgct aaatcgggta aaagcgcaat tgttgaagtg ctgtcgcccg
     6721 gtcaacgccc gactaaacgc ggattaattt acgccgcgac gccagccagc gattttgtct
     6781 gtggcacgca acaggtggct tcgggtatca cagtgcaagt gtttacgacc ggtcgtggta
     6841 cgccgtacgg cctgatggcg gtacccgtca ttaaaatggc aacccgcacc gagctggcga
     6901 accgctggtt tgatttaatg gatattaatg cgggcaccat cgctaccggc gaagaaacta
     6961 ttgaagaggt gggctggaag ttgttccact ttattctcga cgtcgccagc gggaagaaga
     7021 aaaccttctc ggatcaatgg gggctgcata accagctggc ggtgtttaac ccggcaccgg
     7081 tgacctgatt tcttttggaa cgtccctcgc aaaacatggc cttagtgcca tgtttttatt
     7141 gtttaaagcc cccacgtcca ttaataatgc atttgcatta ccttagttca agcttataat
     7201 ttgagcagaa aacaggctgt aaaaggacag tgaatcatgc ccgctaatgc tcgctctcac
     7261 gctgtactga ccactgaatc aaaggtcacg atacgcggac aaacaactat ccccgcgcca
     7321 gtgcgtgagg ccttaaaact gaagccaggc caggacagca ttcattacga aattctgcct
     7381 ggtgggcaag tatttatgtg ccgactggga gatgaacagg aggatcatac tatgaatgca
     7441 tttttgcgtt ttctggatgc agatatccag aacaacccgc aaaaaactcg tccattcaac
     7501 attcaacaag gaaagaaact tgtcgctggc atggacgtca acattgatga tgagattggc
     7561 gacgacgaat aatggatttt ccacaaaggg ttaatggttg ggcgctatat gctcatccct
     7621 gttttcagga aacctacgac gctttagttg ccgaagtcga gacattaaag ggaaaagatc
     7681 ctgaaaatta tcagagaaaa gccgccacaa agttattggc ggtagtccat aaagtgattg
     7741 aggagcatat cacggtcaat ccatcatcac cggcattccg tcatggcaag tcgttaggct
     7801 ctgggaaaaa taaagactgg tcacgggtaa aatttgatgc tggtcgttat cgtctcttct
     7861 ttcgttatag tgaaaaagag aaagtcatca ttctgggatg gatgaacgat gaaaacactc
     7921 tgcgcaccta cggtaaaaaa acagatgcct ataccgtatt cagcaaaatg ttaaaaagag
     7981 gacatcctcc tgccgactgg gaaaccctca cccgagaaac agaagaaacc cattgatggt
     8041 gttcacaatg cccgccgaac gcattcgtgt tgaccagcgg gcatcctttc tcactccccg
     8101 accagaatca cttcaacccc agcctttcgc agtccttcca ggctatccgc aggaatgcct
     8161 tcatcaacaa tgatcatgtc gatacgttga gtatcaatga tcttatgtaa actggaacga
     8221 ttgaacttac tggaatcggt gaccacgatg atccgttccg caacttcgca catccgacgg
     8281 tttaaacggg cttcatcttc attatgtgtg ctgacgccgc gctccagatc gatcgcatct
     8341 acaccaagaa acagcatatc gaagtggtaa ttttgcagcg attgctcagc ctgatcgccg
     8401 taaaaagatt gcgactgacg gcgcaaatgc ccgccggtca tcagcagctc aacgccttcc
     8461 gcttccagca acgcattagc cacgttcata ccgttggtca tcgcaattac gtcagtgtgc
     8521 ttgcgcatca gacgagcaat ctcaaaagtg gtggtcccgg aatcgaggat aacccgatga
     8581 cctggctgaa tcaactcaac ggcagctttc gcaacgctgc gtttcatcgc ggtgttcagt
     8641 gcgcttttat cttccactga tggctcgact gacggcgtcg tgctatcgca gatcaacgcg
     8701 ccaccatagg cacgcacagc gatcccctgc ttttccagaa acgccagatc gttgcggatc
     8761 gtcacagtag atacgccata caatgccgac agatcgttaa cctgcacact cccttgctgt
     8821 cgcagacgct gaatgatctg ttctcgtcgc tcgctggtgc cagtcactcg cttctcacct
     8881 gaagcgtcgg tattactcat agtaagtcct ttcgtaaaac tttcgtttca tttcgttttg
     8941 cctattaacg cctttctatt aagcaaatgc aagcccacct tgcccattga cgcaagctac
     9001 tctcgtttca gtgactttca ttatgtttct tttgtgaatc agatcagaaa actattatct
     9061 ttcgttttat ttttatctca ccatgacgca gtatcaactg aaacaaaacg aaagattaat
     9121 atcgcagtaa tctgaactgg agaggaaagt gaaacatctg acagaaatgg tgagacagca
     9181 caaagcgggc aaaacaaatg gaatttatgc cgtttgttcc gcacatccgc tggtgctgga
     9241 ggctgcaatc cgctacgcca gtgcaaacca aacgccgtta ctgattgaag caacctccaa
     9301 tcaggtagac cagttcggcg gttataccgg aatgacgccc gccgattttc gcggctttgt
     9361 ttgtcagctc gccgactcgt tgaatttccc gcaggatgcg ttgattctgg gtggtgacca
     9421 tctggggcca aaccgctggc aaaacctgcc ggccgctcag gcaatggcca atgccgatga
     9481 tttgattaaa agctacgttg cggcaggatt caaaaaaatc caccttgatt gcagcatgtc
     9541 ctgtcaggac gatccgattc ccttaactga tgacatcgtg gctgaacgcg ccgcccgtct
     9601 ggcgaaagtg gcggaagaaa cctgtcttga acactttggc gaagccgatc tggagtatgt
     9661 cattggtacc gaagtgccgg tacctggcgg cgcgcatgaa accttaagcg agctggcggt
     9721 caccacgccg gatgccgccc gcgccacgct ggaagcccat cgtcacgcct ttgaaaagca
     9781 aggtttgaat gccatctggc cacgcatcat tgccctggtg gttcaacccg gcgtcgaatt
     9841 cgatcacacc aacgttattg attatcagcc cgccaaagcg agcgccttaa gccagatggt
     9901 cgaaaactac gaaacgctga ttttcgaagc gcactctacc gattatcaaa cgccgcaatc
     9961 gctgcgccag ctggtgattg accactttgc cattctgaaa gttggcccag cgctgacctt
    10021 cgccctgcgt gaagctctgt tctctctggc ggcgattgaa gaagaactgg tgccagcgaa
    10081 agcctgttct ggtctgcgtc aggtgctgga agacgtgatg ctcgaccgcc cggaatactg
    10141 gcaaagccac taccacggtg acggcaacgc gcgtcgtctg gcgcgtggtt atagctactc
    10201 ggatcgcgtg cgctattact ggccggacag ccagattgat gacgctttcg ctcatctggt
    10261 acgtaatctg gcggattcac caattccgct gccgctgatc agccagtatc tgccgctgca
    10321 gtacgtgaaa gttcgctccg gcgagctgca gccaacgcca cgggaactca ttatcaacca
    10381 tattcaggac atcctggcgc agtaccacac agcctgtgaa ggccaataag caaaacaaag
    10441 aggaacacgc tatgccaaat attgttttaa gccggattga tgaacgcttg attcacggtc
    10501 aggtcggcgt tcaatgggtc ggatttgcgg gggcaaatct ggtgctggta gccaacgatg
    10561 aggttgccga agatccggta caacaaaacc tgatggaaat ggtactggca gaagggatcg
    10621 ccgtacgttt ctggacgctg caaaaagtta tcgacaacat tcatcgcgcc gccgatcgac
    10681 agaaaatcct gctggtttgt aaaacacccg ccgatttcct gacgctggtg aaaggtggcg
    10741 ttccggtgaa tcgcattaac gttggcaata tgcactacgc caatggcaaa caacaaatcg
    10801 ccaaaacggt ttctgtggat gcgggcgata tcgcagcatt taacgacctg aaagccgctg
    10861 gggtggaatg cttcgttcag ggcgtcccga cagagcctgc tgtggacctc tttaaattac
    10921 tttgagggat tcatcatgga aatcagcctg ttgcaggcat ttgcgttggg cattatcgcc
    10981 tttatcgctg gcctggatat gtttaacggc ctaacacata tgcaccgccc ggtggtcctc
    11041 ggcccgttgg tcgggctggt acttggcgat ctgcataccg gaattttaac cggcggtacg
    11101 ctggaactgg tgtggatggg gctggccccg ctggcgggcg cacagccgcc taacgtgatt
    11161 atcggtacta tcgtcggcac ggcgtttgcc attactactg gcgtgaaacc cgatgtcgca
    11221 gtcggtgtcg ccgtaccttt cgctgtcgca gtacagatgg ggattacctt cctgttctcg
    11281 gtgatgtccg gcgtgatgtc tcgctgcgac cggatggcgg agaacgccga tactcgcggc
    11341 attgaacgcg tgaactatct ggcactgctg gcactcggca ctttctattt tctctgcgct
    11401 ttcctgccta tctacttcgg cgcggaacat gcaaaaacca tcattgatgt cctgccgcaa
    11461 cgattaatcg acggcctcgg cgtcgcgggc ggcatcatgc cagcaatcgg ttttgccgtg
    11521 ctgctgaaaa tcatgatgaa aaacgtctac atcccctact tcatcctggg ctttgttgcc
    11581 gcagcctggc tcaagttacc ggtgctggct attgctgccg ctgcgctggc gatggcgctg
    11641 atcgacctgc tgcgtaaatc tcctgaaccg actcaacctg cggcacagaa agaggaattt
    11701 gaagatggca tctaatcaaa ccaccctgcc gaacgtcagt gaaaacgaag aaacattgct
    11761 gactggcgtc aatgaaaacg tgtatgaaga tcagaacatt ggcgcggagc tgacgaaaaa
    11821 agatatcaac cgcgtcgcct ggcgttccat gctgttacaa gcgtctttta actacgaacg
    11881 tatgcaggct tctggctggt tgtacggtct gctgcctgct ctgaaaaaga tccacactaa
    11941 taaacgcgac ctggcgcgcg ccatgaaggg gcatatgggt ttcttcaata cccatccgtt
    12001 tctggtgaca tttgttatcg gcattatcct tgcgatggag cgttctaagc aggacgttaa
    12061 cagtattcag agcaccaaaa ttgccgtcgg tgcgccgctc ggcgggattg gcgatgcaat
    12121 gttctggcta acgctactgc cgatttgtgg cgggattgga gccagtctgg cattgcaagg
    12181 ctccattctt ggcgcagtcg tctttattgt gctgttcaac gtggtacacc tggggctgcg
    12241 ttttggtctg gcgcattatg cttaccgcat gggcgtggcg gcgattccac tgattaaagc
    12301 gaataccaaa aaagtcggcc atgctgcgtc tatcgttggg atgacggtaa tcggtgcgct
    12361 ggtggcaacc tatgttcgtt taagcaccac gctggaaatc acggcgggcg acgcagtggt
    12421 taagttacag gctgatgtta tcgacaaact gatgccggcc ttcttaccgt tggtctacac
    12481 cctgaccatg ttttggttgg tacgccgcgg ctggagtccg ctgcgtctga ttgcagttac
    12541 cgtggttctc ggcatcgtcg gtaaattctg ccatttcctt taaatacaat gaggtattag
    12601 atgttaagta ttattttgac agggcatggc gggtttgcca gcggcatgga aaaagcaatg
    12661 aagcaaattc tcggtgagca atcgcagttt atcgccatcg attttccgga aacctcaagc
    12721 accgcgctgc ttacatcgca gcttgaagag gccatcgccc aactggattg tgaagacggc
    12781 attgtttttc tgacggattt gctgggcggc acgccgtttc gcgtagcttc aacgctggcg
    12841 atgcaaaaac cgggctgtga agtgatcacc ggcaccaatt tgcaactgct actggagatg
    12901 gtgctggagc gcgaagggtt aagcggtgaa gaattccgtg tacaggcact ggagtgtgga
    12961 catcgtgggc tgaccagtct ggtggacgag ttaggccgct gccatgaaga atgtccggtc
    13021 gaggaaggga tatgacacac gttctgcgcg ccagaaggct gctgactgaa gagggatggc
    13081 tcgatgacca tcagttgcgt attgctgacg gtgtcatcgc agcaatcgaa ccgattccag
    13141 tgggcgtgac tgaacgcgat gcggaactgc tctgccccgc ttacatcgac acccatgtac
    13201 acggtggtgc gggcgttgat gtaatggatg acgcgccgga tgtactcgac aagctggcaa
    13261 tgcacaaggc acgcgaaggt gtcggcagtt ggttgccgac taccgtaacc gcgccgctta
    13321 acaccattca tgcggcgctg aaacgtattg ctcaacgttg ccaacgcggc ggacctggtg
    13381 cgcaagtgct ggggagttat ctcgaaggac cgtacttcac gccgcagaat aaaggcgcgc
    13441 atccgccgga gttgtttcgc gagcttgaaa ttgccgagct ggatcaattg attgccgttt
    13501 ctcagcacac cttacgcgtg gtagcgctgg caccggaaaa agagggggca ttgcaggcca
    13561 tccgccatct taaacagcaa aatgtacgag tgatgctggg gcatagcgcg gcgacctggc
    13621 aacaaactcg cgccgcgttt gatgctggtg ccgacggcct ggtgcattgc tataacggga
    13681 tgacaggttt acatcaccgc gaaccgggaa tggttggcgc gggattaacg gacaagcgcg
    13741 cctggctgga actgatagcc gatggtcatc atgtgcatcc ggcggcgatg tcgctgtgtt
    13801 gttgctgtgc aaaagagaga atcgtgctga tcaccgacgc gatgcaggca gccgggatgc
    13861 cggatggtcg ctatacgtta tgtggcgaag aagtgcagat gcacggtggc gttgtccgta
    13921 ccgcgtccgg tgggctggcg ggcagtacgc tgtctgttga tgcggcagtg cgcaacatgg
    13981 tcgagttgac gggcgtaacg cctgcggaag ctattcatat ggcatcgctg catccggcgc
    14041 gaatgctggg tgttgatggt gttctgggat cgcttaaacc gggcaaacgc gccagcatcg
    14101 ttgcgctgga tagcgggctg catgtgcaac aaatctggat tcagagtcaa ttagcttcgt
    14161 tttgatagtt tgctccttta ttgggccttc acttcccccg taaggccttt ctttttcttt
    14221 cgttttgatc tgtgcagcgg tgtcggatgc gacgctaacg cgtcttatgc gacctacggt
    14281 acgccgcatc cagcaatcgg tgaacatagc gacgtcatta cgcaattctt taatcttccc
    14341 ttgtcttttc tttcaatgat cacagatttc gttttatttc ctttttctcc attgaacttt
    14401 cggtttattt tctatagatt ttaatcaacg aaagacatca ccaagtgaaa tgaaacgaaa
    14461 ggcaagtgaa agcgacaacg cccgacgtca agttcatcag actaaggatt gagttatgcc
    14521 aaaaaattac acccctgctg ccgccgcaac cggtacatgg actgaagaag agatccgcca
    14581 tcagcctcgc gcatggatcc gttcactcac caacatcgac gcgctacgtt ccgcgctcaa
    14641 taacttcctt gaaccgttac tgcgcaaaga gaatctgcgg atcatcctga ccggagccgg
    14701 aacctcggca tttatcggtg acatcatcgc gccgtggctc gccagccata ccggtaaaaa
    14761 cttcagcgcc gtaccgacca ccgatctggt cactaatccg atggactacc tgaacccagc
    14821 ccatccgctg ctgttgatct ccttcggtcg atccggcaac agcccggaaa gcgtcgcagc
    14881 cgtggaactg gcaaatcaat ttgtaccgga atgctatcac ctgccgatca cctgcaacga
    14941 agcgggcgct ctttaccaaa acgcgatcaa cagcgataac gcgtttgccc tgctgatgcc
    15001 cgcagaaacg cacgatcgcg gctttgcgat gaccagcagc attaccacca tgatggccag
    15061 ctgcctcgcg gttttcgcac ctgagacgat caacagccaa accttccgcg acgtggcgga
    15121 tcgttgccag gcgatcctga cctcactggg cgatttcagc gaaggtgtgt ttggttacgc
    15181 accgtggaaa cggatcgttt atctcggtag cggtggctta cagggcgcag cacgcgagtc
    15241 ggcgctgaaa gtgctggaac tgacggcggg taaactggcg gccttttatg attctccaac
    15301 cggattccgt catggaccaa aatcgctggt cgatgacgaa acgctggtgg tggtatttgt
    15361 ctccagccac ccttacaccc gtcagtatga tcttgatctg ctggctgaac ttcgccgtga
    15421 caaccaggca atgcgtgtaa tcgccatcgc cgcggaaagc agcgacatcg tcgctgccgg
    15481 tccacatatc atcctgccac cgtcacgtca ctttatcgac gttgagcagg cattttgctt
    15541 cctgatgtac gcccagacgt ttgcactgat gcagtcgctg cacatgggca atacgccgga
    15601 taccccatca gccagtggca ccgttaaccg cgtggtgcaa ggcgtaatca ttcatccgtg
    15661 gcaggcataa gaggatcgca ttatgagcat tatctccact aaatatctgt tacaggacgc
    15721 ccaggccaat ggctacgcgg tgcctgcttt taacattcat aacgccgaga cgatccaagc
    15781 gatcctcgaa gtgtgcagtg aaatgcgatc gccggtgatc ctcgccggaa cgccggggac
    15841 ctttaaacac atcgcgctgg aagagatcta cgccctgtgt agcgcctatt ccacaaccta
    15901 caacatgcca ctggcgctgc atctcgacca ccacgaatcg ctggatgata ttcgccgtaa
    15961 agtccacgca ggtgtgcgca gtgcgatgat cgacggcagc cacttcccgt ttgccgagaa
    16021 cgtgaagctg gtgaaatcgg ttgttgactt ctgccactca caagattgca gcgtggaagc
    16081 agaactgggc cgcctgggcg gtgttgaaga tgacatgagc gttgacgccg aaagtgcatt
    16141 cctgaccgat ccacaagaag ctaaacgctt tgtcgaactg actggcgtcg acagcctggc
    16201 ggtagcgatt ggtacggcgc acggcttata cagcaaaacg ccgaagattg atttccagcg
    16261 gctggcggaa attcgtgaag tggtggatgt tcctctggtg ctgcatggtg ccagcgatgt
    16321 tccggatgaa tttgtccgtc gcactattga acttggcgtc acaaaagtga acgttgccac
    16381 agaattaaaa atagccttcg ctggcgcggt taaagcctgg tttgcggaaa atccgcaggg
    16441 taatgatcct cgttattata tgcgcgtcgg aatggatgcg atgaaagaag ttgtcagaaa
    16501 taaaattaat gtctgtggtt cagcgaatcg aatttcagca taatcattag ttttttatga
    16561 tttatcccaa tgtacttccc gattaatatc ggggagtgcc ttaatggaaa aggagataac
    16621 taaaccttaa taaatacatc agtacaatat cgcaacaata atatatttaa aaaaattata
    16681 ttattcaact ttatggtgag gattacacaa tgagcagtcc aaatattctc ttaacccgta
    16741 ttgataaccg tctggttc
//
LOCUS       DAOFQD010000105        16565 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.105, whole genome shotgun sequence.
ACCESSION   DAOFQD010000105 DAOFQD010000000
VERSION     DAOFQD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.105"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            54..130
                     /locus_tag="QBF46_003510"
     tRNA            54..130
                     /locus_tag="QBF46_003510"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Pro,seq:tgg)
     gene            277..1512
                     /locus_tag="QBF46_003511"
     CDS             277..1512
                     /locus_tag="QBF46_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDQ6675649.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1596..1757)
                     /locus_tag="QBF46_003512"
     CDS             complement(1596..1757)
                     /locus_tag="QBF46_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675650.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(2436..3632)
                     /gene="hemY"
                     /locus_tag="QBF46_003513"
     CDS             complement(2436..3632)
                     /gene="hemY"
                     /locus_tag="QBF46_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDQ6675651.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIVPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(3635..4864)
                     /gene="hemX"
                     /locus_tag="QBF46_003514"
     CDS             complement(3635..4864)
                     /gene="hemX"
                     /locus_tag="QBF46_003514"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDQ6675652.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYYTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            complement(4886..5626)
                     /gene="hemD"
                     /locus_tag="QBF46_003515"
     CDS             complement(4886..5626)
                     /gene="hemD"
                     /locus_tag="QBF46_003515"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDQ6675653.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQAREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(5623..6564)
                     /gene="hemC"
                     /locus_tag="QBF46_003516"
     CDS             complement(5623..6564)
                     /gene="hemC"
                     /locus_tag="QBF46_003516"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDQ6675654.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            6951..9497
                     /gene="cyaA"
                     /locus_tag="QBF46_003517"
     CDS             6951..9497
                     /gene="cyaA"
                     /locus_tag="QBF46_003517"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDQ6675655.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(9537..9857)
                     /gene="cyaY"
                     /locus_tag="QBF46_003518"
     CDS             complement(9537..9857)
                     /gene="cyaY"
                     /locus_tag="QBF46_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDQ6675656.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            10320..10523
                     /locus_tag="QBF46_003519"
     CDS             10320..10523
                     /locus_tag="QBF46_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6675657.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            10560..11384
                     /gene="dapF"
                     /locus_tag="QBF46_003520"
     CDS             10560..11384
                     /gene="dapF"
                     /locus_tag="QBF46_003520"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDQ6675658.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11381..12088
                     /locus_tag="QBF46_003521"
     CDS             11381..12088
                     /locus_tag="QBF46_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDQ6675659.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12085..12981
                     /gene="xerC"
                     /locus_tag="QBF46_003522"
     CDS             12085..12981
                     /gene="xerC"
                     /locus_tag="QBF46_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDQ6675660.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            12981..13697
                     /gene="yigB"
                     /locus_tag="QBF46_003523"
     CDS             12981..13697
                     /gene="yigB"
                     /locus_tag="QBF46_003523"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDQ6675661.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            13781..15943
                     /gene="uvrD"
                     /locus_tag="QBF46_003524"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             13781..15943
                     /gene="uvrD"
                     /locus_tag="QBF46_003524"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDQ6675662.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16090..>16565)
                     /locus_tag="QBF46_003525"
     CDS             complement(16090..>16565)
                     /locus_tag="QBF46_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDQ6675663.1"
                     /translation="YAPLGLYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIV
                     RLDAFKASKDIQFAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQ
                     QATNFYDFACYAKAKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3785 a         4289 c         4498 g         3993 t
ORIGIN      
        1 ccctcgcacc acgactttaa agaattgaac taaaaattca aaaagcagta tttcggcgag
       61 tagcgcagct tggtagcgca actggtttgg gaccagtggg tcggaggttc gaatcctctc
      121 tcgccgacca attttgaacc ccgcttcggc ggggtttttt gttttctgtg catttcgtca
      181 ccctcccttc gcaataaacg cccgtaataa ctcattgccc cacggtatga tttcgccctt
      241 aacgtattga aggatgactt caggcaagga gtgaccatgc tgcaacaggt tccaacgcgt
      301 gcttttcatg tgatggcgaa accgagtggt tccgattgta atctgaactg tgactactgt
      361 ttttatctcg aaaaacaatc cctttaccgc gaaaagccag tcacgcatat ggacgatgac
      421 acgctggaag cgtatgtccg tcactatatc gctgccagcg aaccgcaaaa cgaagtggct
      481 tttacctggc agggcggcga accaacgtta ctcgggctgg atttttaccg ccgtgccgta
      541 aagttacagg cgaaatacgg tgctggcagg aagataagta acagcttcca gactaacggc
      601 gtgctgctcg atgataaatg gtgtgcattt ctggcagaaa atcattttct tgttgggtta
      661 tcgctggacg gtccggctga gatccacaat caatatcgcg tgaccaaagg tggcagacca
      721 acgcataagc tggtgatgcg tgccctgacg ctgctgcaaa aacatcatgt cgactataac
      781 gtgctggtct gcgtcaaccg caccagcgcg cagcaaccgt tgcaggttta tgattttttg
      841 tgcgatgcgg gagtcgaatt catccagttt attccggtgg tcgagcgtct ggctgatgaa
      901 acagctgccc gcgatggact gaaattgcat gcgcctggtg atattcaggg ggaactgacg
      961 gaatggtcgg tgcgccccga tgaatttggt gaatttctgg tggcgatatt cgaccactgg
     1021 atcaaacgcg acgtcggcaa gattttcgtg atgaatatcg aatgggcgtt tgccaatttt
     1081 gtcggtgcgc cgggtgcggt ttgccatcat cagccaacct gtgggcgctc ggtgattgtt
     1141 gagcacaacg gcgacgttta cgcctgcgat cactatgttt atccgcaata tcggctgggg
     1201 aatatgcatc agcaaacaat tgcagaaatg atcgattccc cgcaacagca ggtgtttggt
     1261 gaagataaat ttaagcaatt accggcgcag tgtcgcagtt gtaacgtgtt aaaagcgtgc
     1321 tggggaggct gcccgaaaca ccgcttcatg ctcgatgcca gcggcaaacc ggggctgaat
     1381 tatttgtgtg ccgggtatca gcgttatttc cgccatctac cgccatatct taaagcaatg
     1441 gctgatttgc tggcgcacgg tcgcccggcc agtgacatta tgcatgcgca tttgctggtg
     1501 gtgagtaagt aaaaaagcgg ccattttgtg gccgcctgcg gttgattgcc ggatgcggcg
     1561 taaacgcctt atccggccta catgatcgtg caaattcaat aaattgcagc gttctgtgga
     1621 tactggtttg ggtgatcgta tccggcaaag cctttgcgtg cggtggcagt tgacggggta
     1681 tctgctgcgc tggccatgag aggaagagcg gcggcgacag caacaacaag aagtttgggt
     1741 gaaaacgaaa attccatgca aaatgctccg gtttcatgtc atcaaaatga tgacgtaatt
     1801 aagcattgat aattgagatc cctctccctg acaggatgat tacataaata atagtgacaa
     1861 aaataaatta tttatttatc cagaaaatga attggaaaat caggagagcg ttttcaatcc
     1921 tacctctggc gcagttgata tgtaaggcag gtttattata tcgcgttgat tattgatgct
     1981 ttttttagtt ttaacggcaa ttaatatatg tgttattaat tgaatgaatt ttatcattca
     2041 taataagtat gtgtaggatc aagctcaggt taaatattca ctcaggaagt tattactcag
     2101 gaagcaaaga ggattacaga attatctcat aacaagtgtt aagggatgtt atttcccgat
     2161 tctctgtggc ataataaacg ggtagatgct cattccatct cttatgttcg ccttagtgcc
     2221 tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact gacagatgtc
     2281 gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac ttgttgtcat
     2341 acagacctgt tttaacgcct gctccgtaat aagagcaggc gtttttttat gcatcagaaa
     2401 ggtcccggag gtgcttgcct ccgggtgaga aggaactact gtggtgggtt gttctgcaac
     2461 gttaacatca aaccgtcgcg acgcatcgct gcagcttctt ccggcttgtg cagtctgtcc
     2521 agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag cgccgcgcgg
     2581 aaggcgagcg atgcttcctg ccattctccg tgcttcatca gtgactggcc cagtgtgctc
     2641 cacaacagcg ggcgatcgcc gacgtttttg atctgctggc gtagcacttt ttccagctgt
     2701 tccgggttat tggttttcag acgcgggatc ggcagcagta ggcgatcgtc atactggcgt
     2761 ttcaggccgt cgatgataat ttgctgggca gtatcatgat cgtcacattc aataagatgt
     2821 tccgccattg ccacctgcaa cgctacctga tgacgcgttt tccggctttg gtttttccac
     2881 cagttacgca aaccttcgct accgttatcg gcacgagcct gatccatcag gccaatccat
     2941 gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt cgccattgat
     3001 gggacaatat ccagcagcga actccatgca cctgtgcgga tatacgcctg ttccgccaga
     3061 cgtaatactt ccggatggcg tggcgtaact tccagcagct tatccacgcc gtggcgtgca
     3121 gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac cggaatggtg
     3181 tcattgccgg ccagctccgc tgcgcgttcc agatgttggt tggcgcgcgc ttcatcacca
     3241 cgttgttgcg cggcttcggc agccagtaga tagttcacca ccggttgttc agcgtgatcg
     3301 gcatttttcg ccatcagctt ttcaacttgc tgataatcgc cttccgccag tttcagcagc
     3361 gcctgttcgg tctgcttacg tgcacggcga cgcttacgcc cgacaaacca cccacgggtg
     3421 tgcgcgccag tgcggaagat ccgccgcagt agccactcaa tggcaaacag cactaccatc
     3481 gccagaatca atatgatcgc caggcccgtg acgctggttt cgatattgta gttgtcggtt
     3541 tggatcagca cataaccctg atgaccggca atcatcgggc caaccactat ccccgcaatc
     3601 agcaaaacaa agagtaataa cacttttagc atggttattc tccttgcggc gcggctgccg
     3661 gagtatcacc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtg
     3721 caggtgcagg tgcaggttta gcttccgttg tccctgctgc cggttgtgcc agcaggttac
     3781 gcacgcgagt ctgcatcagt ttttccagca tcgcctggct ttgcagggtt tccggaagat
     3841 ccatcgagat attttgctgg cttaactggt ccacctcgtc gaggaacgct ttggtggtgg
     3901 catcatcagt atagtagtaa gcacgtaccc aggtggagac gttctccagc gcctggcgat
     3961 aagtctcttc ctggtgacgc ggtacagctt gtgctgcgac cagcaggcga gagcgaatat
     4021 tttcgcgcag atagatatcc tgatttggcg ctaacagcgg tacggcggtg tcatcacgac
     4081 ggcgaatcgt aatgaagttg tccataaagt tctgccagct tttttgcaga ttgatacgcc
     4141 attcgctgat ggaactggaa agctcttcac cgtctgaatc catcggcgaa ccatcgctgt
     4201 cattatcggc cagacgcagg ttatctacct gatttgaaag ctgattaagc ttaaggatga
     4261 tgccgtcata atccacctgc gatactgcag aaaggctggc gatatcatcg gtaattgccc
     4321 gacgaacggt aatcagactc gggtcattca tatccgccag gctggcgtct gcacttttca
     4381 gcaacgctgc agcggtcgtg acgtcctgat cgctccacag cttccgtccg gcgagtttca
     4441 ccagaaaatc ggcctgagcc agcagccagg ttttagcatc gctgccggaa atggtggcga
     4501 ccttttgttg gacttcatcc aactgttttg ccagcgtttc ttgctgacga ttcgcctgct
     4561 taagttgtgc agcttgttgc ttaataatgc cttccagctc ggctttttgg ctctcctggg
     4621 ctttttgcaa tgccgtcagt tggttagcca gggcatcgct ggtggcggtc tgattgacgg
     4681 cctgttgttt accccagcca tacaaaccga cgcccgccgc cagagcaata gcgatagcca
     4741 ccgcgctgag aatcaatgcg gtattgttct tactcttttt ttctgttgcg acaggttgtg
     4801 acgtggtgtc cacggcctcc ctggtctctt caaccacggc ggaggttttt tcttgttccg
     4861 tcattatggc ttcctgttat gagagttatt gtaatgcccg taaaagcgca tcgttgtcag
     4921 cgttatcggc gaccttaatg tcttgccagc ccagttcccg ggcgagtttc gccaaacgct
     4981 cactgacgac caatagtcga cagtgtagta accagtgctc acgataccat tgtgggatca
     5041 gcgaccagag ttgctgcaac atttcaccgc tggtaacaac gaccatcgtc acctcgcggg
     5101 cttgccagcg catcgcttct tctgcaccat cgtaatggat tgcgcatcgt tgataacatt
     5161 cacaaaaagt gacctcagca ccgcgcgccg tcagggtatc cccaattagc tcacgaccac
     5221 cattgccacg taatatcagc gcacgtttgc ccgcaatatt ttgtaattca ggtaattgta
     5281 gcaagacttc gctgatttcc cgatcctgcg ggtagagaat cttctgtccg cttacggtat
     5341 gtagtgccag tgcggtggtg cgtccaatgg cgaaataatc aggtagtcgg ggccatttac
     5401 gatcttgctg atgcagctgt gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca
     5461 acagatcgct ctcccccagc gctgccagtt gatcagcaag ttgcggtaat tgttgacccg
     5521 gagaaaactc aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc agacggctca
     5581 ctaactcttc tccagcggga gacgggcggg tgacaaggat actcatgccg gggcgtctcc
     5641 gttatagact tcagcgagga tctcgcgcgc gccgttattc agtagctctt ctgccagcga
     5701 aatccccatt tgttcggcat tttgcggcgc accgcggcgt tcaccgcgaa taatctgcga
     5761 accgtccggc gcgccgacca gcgcacgcag ccagatttcg ccatcaataa gctcggcgta
     5821 gctaccaatt ggcacctgac atccgccttc gagacgggta ttcatggcgc gttctgcggt
     5881 aacgcgcagt gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag tgcgcgtatc
     5941 atcaaggcgg cattcaatac cgactgcacc ttgtcctacc gccggaagag aaatctcggg
     6001 tggtaacgcg gcgcgaatgc gggactccag acctaaacgt tttagtccgg ctacggcaag
     6061 aatgatggca tcgtattcgc cgttatctag tttgctcagg cgagtgccga cgttgccgcg
     6121 cagggagcgg ataatcagat ccgggcggcg ttcagccagt tggcactggc gacgtaaact
     6181 ggacgtcccg acgatactgc ctgccggtaa cgcatccaga ctgtcatagt tattggacac
     6241 aaaggcatcg cgaggatctt cacgctcaca aatagtgacc agtcccagac cttgcgggaa
     6301 ttcaaccggc acatctttca ttgagtgtac ggcgatatcg gcgcgatttt cgaggagcgc
     6361 gacttccagc tcttttacaa ataagccttt tccgcctact ttcgccagcg gcgtatcaag
     6421 aatcacatcg ccgcgcgtca ccatcggtac cagttcaacg accaggcccg gatggctcgc
     6481 catcaacttg tctttgacat agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc
     6541 aattcttaaa acattgtcta acatgcttgt taccgtcatt atcatccgtg gtccatccta
     6601 acatccttgc cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta
     6661 aaatgaggtc tggcagtgga tcctgacagg cgtttcacgc cgtcgtaata aggaatttac
     6721 agagaataaa cggtgctaca cttgtatgta gcgcatcttt ctttacggtc aatcagcaag
     6781 gtgttaaatt gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc
     6841 gaaaagtggt aacggttacc tttgacatac gaaatatccc gaatgccgcg tgttaccgtt
     6901 gatgttggcg gaatcacagt catgacgggt agcaaatcag gcgatacgtc ttgtacctct
     6961 atattgagac tctgaaacag agactggatg ccataaatca attgcgtgtg gatcgcgcgc
     7021 ttgctgctat ggggcctgca ttccaacagg tctacagtct actgccgaca ttgttgcact
     7081 atcaccatcc gctaatgccg ggttaccttg atggtaacgt tcccaaaggc atttgccttt
     7141 acacgcctga tgaaactcaa cgccactacc tgaacgagct tgaactgtat cgtggaatgt
     7201 cagtacagga cccgccgaaa ggtgagcttc caattactgg tgtatacacc atgggcagca
     7261 cctcgtccgt agggcaaagt tgttcctctg acctggatat ctgggtctgt catcaatcct
     7321 ggctcgatag cgaagagcgc cagttactac aacgtaaatg cagcctgctg gaaagctggg
     7381 ccgcctcgct gggcgtggaa gttagcttct tcctgattga tgaaaaccgc ttccgtcata
     7441 atgaaagcgg cagcctgggg ggcgaagatt gtggctccac ccagcatata ctgctgcttg
     7501 acgaatttta tcgtaccgcc gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc
     7561 cgtgcgacga agaagagcat tacgacgact atgtgatgac gctttacgcg cagggcgtgc
     7621 tgacgccaaa tgaatggctg gatctcggtg gcttaagctc gctttctgct gaagagtact
     7681 ttggtgccag cctttggcag ctctacaaga gtatcgattc cccatacaaa gcggtgctga
     7741 aaacactgct gctggaagcc tattcctggg aatacccgaa cccacgtctg ctggcgaaag
     7801 atatcaaaca gcgtttgcac gacggcgaga ttgtatcgtt tggcctcgat ccgtactgca
     7861 tgatgctgga gcgtgttact gaatacctga cggcgattga agatttcacc cgtctggatt
     7921 tagtacgtcg ctgcttctat ttaaaagtgt gcgaaaagct cagccgtgaa cgcgcctgcg
     7981 taggctggcg tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg gacgaagctc
     8041 gtctggcaat gctcgataac cgcgctaact ggaagattga tcaggtgcgt gaggcgcaca
     8101 acgagttgct cgacgcgatg atgcagagct accgtaatct gatccgcttt gcgcgtcgca
     8161 ataaccttag cgtctccgcc agtccgcagg atatcggcgt gctgacgcgt aagctgtatg
     8221 ccgcgtttga agcattacca ggtaaagtga cgctggtaaa cccgcagatt tcacccgatc
     8281 tctcggaacc gaatctgacc tttatttatg tgccgccggg ccgtgctaac cgttcaggtt
     8341 ggtatctgta taaccgcgcg ccaaatattg agtcgatcat cagccatcag ccgctggaat
     8401 ataaccgtta cctgaacaaa ctggtggcgt gggcatggtt taacggcctg ctgacctcgc
     8461 gcacccgttt gtatattaaa ggtaacggca ttgtcgattt gcctaagttg caggagatgg
     8521 tcgccgacgt gtcgcaccat ttcccgctgc gcttacctgc accgacaccg aaggcgctct
     8581 acagcccgtg tgagatccgc catctggcga ttatcgttaa cctggaatat gacccgacag
     8641 cggcgttccg caatcaggtg gtgcattttg atttccgtaa gctggatgtc ttcagctttg
     8701 gcgagaatca aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac tcgtggaacg
     8761 aagtgcgtac gctgcacttc aacggcgagc aatcgatgat cgaagccctg aaaactattc
     8821 tcggcaaaat gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc
     8881 agcatctgcg cggcttaatc cgtactcgcg tgcagcaact ggtttctgag tgtattgaac
     8941 tgcgcctttc cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc gtttctggtc
     9001 aaacctgggg gttgttcttc gaacgcctga atgtatcggt acaaaaactg gaaaacgcca
     9061 tcgagtttta tggcgctatt tcgcataaca aactgcacgg cttgtcagtg caggttgaaa
     9121 ccaatcacgt caaattaccg gcggtggtgg acggctttgc cagcgaaggg atcatccagt
     9181 tctttttcga agaaacgcaa gacgagaatg gctttaatat ctacattctc gacgaaagca
     9241 accgggttga ggtgtatcac cactgcgaag gcagcaaaga ggaactggtg cgtgacgtca
     9301 gtcgcttcta ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc atcaacttca
     9361 acctgccgca gttctatcag attgtgaagg ttgatggtcg cgaacaggtg attccgttcc
     9421 gaaccaaatc tatcggcaac atgccgcccg ccaatcagga tcacgatacg ccgctattac
     9481 agcagtattt ttcgtgatga gcgtgccgga aagcgaggct tatccggcat gcaatcttag
     9541 cggaaactga ctgtttcacc cgcttgctgt gtcgccgcct gttccagcaa atcccagaag
     9601 gtttcgccgc tgcgatcaca aatccactca tcgcctttca ggtcaaaatg gtagccgccc
     9661 tgtttggttg ccagccatac ctggtgcagc ggctcctggc ggttgataat gattttgctg
     9721 ccattctcaa aggtaatggt cagtacgccg ccgttgattt cgcagtcgat atcgctgtcg
     9781 ccatcccagt cgtccaggcg ttcttcaatg gtcagccaga gttgatcagc caggcgatga
     9841 aattcactgt cgttcattgt tgtatcctgt ttttaagtga tggcggcagt atagcggcat
     9901 ggggtcaggg cttcaaagtt tgcacctctg cggctgcgtt ccggcacgat tcatccgtca
     9961 ccggaataat gatgtctctg tgtagcgaaa gatttgtctc ttcattaggg cgcagttaca
    10021 ccacgtcttt ccctgtttct ggtaaacatt atgatcaggt ttaccgagcg agcatcctca
    10081 cgctgacgga actaaaaaag acaacaaaca aaccacattg cgatagtgca taaagccatc
    10141 ctggcgcgag gtgccgatca cgaaactacc agcaaaacat aaatccccac gagtaagcgt
    10201 tatactcgca gcatttcctc acttttcaga cttcataaag agtcgctaaa cgcttgcttt
    10261 tacgtcttct cctgcgatga tagaaagcag aaagcgatga actttacagg caatccataa
    10321 tgaaaaacgt gtttaaggca ctcactgtat tacttactct cttcagcctg acgggctgcg
    10381 gtctgaaagg tccgctctat ttcccgcctg cagataaaaa cgcaccgccg ccgaccaaac
    10441 cggtagagac gcaaacgcaa tccacggtgc cggataaaaa cgatcgcgcc actggcgatg
    10501 gtccatccca ggtgaattac taaaagtcag tttctgtacc cgcgtgattg gagtaaatga
    10561 tgcagttctc gaaaatgcat ggccttggca acgattttat ggtcgtcgac gcggtaacgc
    10621 agaatgtctt tttttcaccg gagctgattc gtcgcctggc tgatcggcac ctgggggtag
    10681 ggtttgacca actgctggtg gttgagccgc cgtatgatcc tgaactggat tttcactatc
    10741 gcattttcaa tgctgatggc agtgaagtgg cgcagtgcgg caacggtgcg cgctgctttg
    10801 cccgttttgt gcgtctgaaa ggactgacca ataagcgtga tatccgcgtc agcaccgcca
    10861 acgggcggat ggttctgacc gtcaccgatg atgatctggt ccgcgtaaat atgggcgaac
    10921 ccaacttcga accttccgcc gtgccgtttc gcgctaacaa agcggaaaag acctatatta
    10981 tgcgcgccgc cgagcagaca atcttatgcg gcgtggtgtc gatgggaaat ccgcattgcg
    11041 tgattcaggt cgatgatgtc gataccgcgg cggtagaaac gcttggtcct gttctggaaa
    11101 gccacgagcg ttttccggag cgcgccaata tcggttttat gcaagtggtt aagcgcgagc
    11161 atattcgttt acgcgtttat gagcgtgggg caggagaaac ccaggcctgc ggcagcggcg
    11221 cgtgtgcggc ggttgcagta gggattcagc aaggtttgct ggccgaagaa gtacgcgtgg
    11281 aactccccgg cggtcgtctt gatatcgcct ggaaaggtcc gggtcacccg ttatatatga
    11341 ctggcccggc ggtacatgtc tacgacggat ttattcatct atgaagcaac caggggaaga
    11401 actgcaggaa acactcacgg agcttgatga ccgggcggtt gtcgattatc tgattaaaaa
    11461 tcctgagttt tttatccgta atgcgcgcgc agtagaagcg atacgtgtgc cgcatccggt
    11521 acgcggcacc gtttcgttgg tcgagtggca catggcccgc gcacgtaatc atattcatgt
    11581 tctggaagag aacatggcgc tgttgatgga acaggctatc gccaacgaag gcctgtttta
    11641 tcgcctactc tacctgcagc gcagtctcac cgccgccagc agtctcgacg atatgctgat
    11701 gcgctttcac cgctgggcgc gcgatctcgg cctggcaggt gcgagtctgc gcctgtttcc
    11761 ggatcgctgg cgcttaggtg cgccgtcgaa ccacactcat ctggcattaa gccgtcagtc
    11821 tttcgaaccg ctgcgtattc agcgtttggg gcaggaacag cactatcttg ggccgcttaa
    11881 cggaccagag ctgctggtgg tgctaccgga agcgaaagcg gtgggatcgg tggcgatgtc
    11941 gatgctggga agcgatgctg atttgggtgt cgtgctgttt accagtcgcg atgccagtca
    12001 ctatcaacaa gggcagggaa cgcagttact tcatgaaatt gcgctgatgt tgccggagct
    12061 tctggagcgt tggattgaac gcgtatgacc gatttacaca ccgatgtaga acgctaccta
    12121 cgttatctga gcgtggagcg ccagcttagc ccgataaccc tgcttaacta ccagcgtcag
    12181 cttgaggcga tcatcaattt tgccagcgaa aacggcctgc aaagctggca acaatgcgat
    12241 gcagcgatgg tacgcaattt tgctgtacgc agtcgccgta aagggctggg agcagcaagt
    12301 ctggcgttac ggctttctgc gctacgtagc ttttttgact ggctggtcag ccagaacgaa
    12361 ctcaaagcta acccggcgaa aggtgtttcg gcaccgaaag cgccgcgtca tctgccgaaa
    12421 aatatcgacg tcgacgatat gaatcggctg ctggatattg atatcaacga tcccctcgcc
    12481 gtgcgcgacc gggcaatgct ggaagtgatg tacggcgcgg gcctgcgtct ttccgaactg
    12541 gtggggctgg atatcaaaca cctcgacctg gagtccggcg aagtgtgggt gatggggaaa
    12601 ggcagcaaag agcgccgcct gccgattggt cgcaacgctg tggcgtggat tgagcactgg
    12661 cttgatttgc gcgacctgtt tggtagcgaa gacgacgcgc tttttctgtc gaaactgggc
    12721 aagcgtatct ccgcgcgtaa tgtgcagaaa cgctttgccg aatggggcat aaaacaaggg
    12781 ctgaataatc acgttcaccc gcataaatta cgtcactcgt tcgctacgca tatgctggag
    12841 tcgagcggcg atcttcgtgg tgtgcaggag ctgctgggtc atgccaacct ctccaccacg
    12901 caaatctata ctcatcttga ttttcaacac cttgcctcgg tgtacgatgc ggcgcatcca
    12961 cgcgccaaac gggggaaata atgcgttttt accggccttt ggggcgcatc tcggcgctca
    13021 cctttgacct ggatgatacc ctttacgata accgtccggt gattttgcgc accgagcgag
    13081 aggcgcttac ctttgtgcaa aattatcatc cggcgctgcg cagcttccag aatgaagatc
    13141 tgcaacgcct gcgccaggcg gtacgggaag cggaacccga gatttatcac gacgtgacgc
    13201 gctggcgttt tcgttcgatt gaacaagcga tgctcgacgc cgggctgagt gccgaagaag
    13261 ccagtgccgg cgcacacgca gcaatgatca actttgccaa atggcgcagc cggatcgacg
    13321 tcccgcagca aacccacgac accttaaaac agctggcgaa gaaatggccg ctggtggcga
    13381 tcaccaacgg taacgcccag ccggagctgt ttggtttggg ggattatttt gagtttgtgc
    13441 tgcgcgctgg cccgcacggg cgctcaaaac cgttcagcga tatgtacttt ctggcggcgg
    13501 aaaaactcaa cgtgccgatc ggcgagatct tacatgttgg ggacgatctc accactgacg
    13561 tgggtggggc aattcgcagc ggaatgcagg cttgttggat cagaccggaa aatggcgatc
    13621 tgatgcaaac ctgggacagc cgtttactgc cacatctgga aatttcccgg ttggcatctc
    13681 tgacctcgct gatataatca gcaaatctgt atatataccc agctttttgg cggagggcgt
    13741 tgcgcttctc cgcccaacct atttttacgc ggcggtgcca atggacgttt cttacctgct
    13801 cgacagcctt aatgacaaac agcgcgaagc ggtggccgcg ccacgcagca accttctggt
    13861 gctggcgggc gcgggcagtg gtaagacgcg cgtactggtg catcgtatcg cctggctgat
    13921 gagcgtggaa aactgctcgc catactcgat tatggcggtg acgtttacca acaaagcggc
    13981 ggcggagatg cgtcatcgta tcgggcaact gatgggcacc agccagggcg gtatgtgggt
    14041 cggcaccttc cacgggctgg cgcaccgcct gctgcgtgcg caccatatgg acgccaatct
    14101 gccgcaggat ttccagatcc tcgacagtga agaccagcta cgcctgctta agcgtttgat
    14161 caaggcgatg aacctcgacg agaagcagtg gccgccgcgc caggcaatgt ggtacatcaa
    14221 cagccagaaa gatgaaggcc tgcgtccgca tcatattcaa agctacggta atccggtgga
    14281 gcagacctgg cagaaggtgt atcaggcgta tcaggaagcg tgcgatcgtg cgggtctggt
    14341 ggacttcgcc gagctgctgc tgcgcgctca cgagttgtgg cttaacaagc cgcatatcct
    14401 gcaacactac cgcgaacgtt ttaccaatat cctggtggac gaattccagg ataccaacaa
    14461 cattcagtac gcgtggatcc gcctgctggc gggcgatacc ggcaaagtga tgatcgttgg
    14521 cgatgacgac cagtcaatct acggctggcg cggggcgcag gtagagaata ttcagcgttt
    14581 cctcaatgat ttccccggtg ctgaaactat ccgtctggag cagaactacc gctctaccag
    14641 caatattctg agcgccgcta acgccctgat tgaaaacaac aatgggcgtc tgggtaaaaa
    14701 attgtggacc gatggcgcgg acggtgagcc gatttccctc tattgcgctt ttaacgaact
    14761 cgacgaagcg cgttttgtgg ttaatcgcat caaaacctgg caggacaacg gcggggcgct
    14821 tgccgagtgc gccattctct accgcagtaa cgcccagtcg cgtgtgctgg aagaagcact
    14881 gttgcaggcg agtatgccgt accgtattta cggcgggatg cgcttcttcg aacgccagga
    14941 aatcaaagat gcgctctcgt atctgcgcct gattgccaac cgcaacgacg acgcggcctt
    15001 tgagcgcgta gtgaatacac caacgcgggg tattggtgac cggacgctgg acgtggtacg
    15061 tcagacatcg cgcgatcgcc agttaacact ctggcaggca tgtcgtgaac tgttgcagga
    15121 aaaagccctc gccggacgtg ctgccagcgc cttacagcgg tttatggaac tgatcgacgc
    15181 cttagcgcag gaaactgccg atatgccgct gcatgtacag actgaccggg taattaaaga
    15241 ctccggcctg cgtaccatgt atgagcagga gaagggcgaa aaaggtcaga cgcgtatcga
    15301 aaacttagag gaactggtga cggcaacgcg ccagttcagc tacaacgaag aagacgaaga
    15361 tttaatgccg ctgcaggcgt tcctctccca tgcggcactg gaagcaggtg aagggcaggc
    15421 ggatacctgg caggatgcgg tgcagttgat gacgctacac tcggcgaaag gcctggagtt
    15481 cccgcaggtg tttatcgttg gtatggaaga gggcatgttc ccaagccaga tgtcgctgga
    15541 tgaaggcggg cgtctggaag aagaacgccg tctggcctac gttggcgtaa cccgcgcgat
    15601 gcagaaactg acgctgacct acgcggaaac ccgccgtctg tatggtaaag aggtttacca
    15661 tcgcccgtcg cgctttatcg gcgagctgcc ggaagagtgt gtggaagagg tgcgcctgcg
    15721 cgccacggta agccgcccgg tcagccatca gcggatgggt acgccgatgg tcgagaacga
    15781 cagcggctac aagctcggcc agcgcgtacg ccacgctaag tttggtgaag gcaccattgt
    15841 caatatggaa ggcagcggtg agcatagccg tttgcaggtg gcatttcagg gccagggtat
    15901 taaatggctg gtggcggcat acgcccggct ggagtcggtg taacgttgcc ggatgcggtg
    15961 ctgcgcacct tatttggcct aaaaaatcat tcatattcaa taaattgcaa cgtcatgtat
    16021 gccggatagg gcgtttacgc cgcatccggc atctgcgcca tcttcagtat ctgacacaaa
    16081 actatcgttt taacctttcc gctcgacgga aatcatggtg acaaaaggat agcgttgcca
    16141 cgggattgcc ccgcctttca tatacatatg tgaaatcgta ccatcaagat aaagcagctg
    16201 ctcaacgttc agtttcgctt tggcataaca ggcaaaatca taaaaatttg tcgcctgctg
    16261 gctcaacaaa aacacggcat tcccatgttt attaatccca acaccgttac gaattttacg
    16321 tgaggcgacg ttgggatgaa tacgcggatt aattacaccg ttttccatca acattggccc
    16381 tgactgcacc gcaaactgaa tatctttact ggctttaaaa gcatctagac gaacgatgcc
    16441 gactttatct cccgcgacat aaaacacgcc gccaggacgg ataaagaaat tcccttcacc
    16501 tgaagcgaga tttaacgcca ccttctgctg accgttttcg atgtacaaac cgagcggcgc
    16561 atagc
//
LOCUS       DAOFQD010000106        16556 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.106, whole genome shotgun sequence.
ACCESSION   DAOFQD010000106 DAOFQD010000000
VERSION     DAOFQD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.106"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            2..166
                     /locus_tag="QBF46_003526"
     CDS             2..166
                     /locus_tag="QBF46_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015386348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675664.1"
                     /translation="MSCSLLLNPLTTYARNYPCSGKKGGVSHCTSDGKFVCNDGTISK
                     SKKICTKNSR"
     gene            355..1407
                     /locus_tag="QBF46_003527"
     CDS             355..1407
                     /locus_tag="QBF46_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6675665.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAVNLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDYYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLPSGDKVAAAVGITP"
     gene            complement(1512..2048)
                     /gene="rcdA"
                     /locus_tag="QBF46_003528"
     CDS             complement(1512..2048)
                     /gene="rcdA"
                     /locus_tag="QBF46_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDQ6675666.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            2132..3340
                     /locus_tag="QBF46_003529"
     CDS             2132..3340
                     /locus_tag="QBF46_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6675667.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            3340..4155
                     /gene="ybjI"
                     /locus_tag="QBF46_003530"
     CDS             3340..4155
                     /gene="ybjI"
                     /locus_tag="QBF46_003530"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDQ6675668.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            4241..4525
                     /locus_tag="QBF46_003531"
     CDS             4241..4525
                     /locus_tag="QBF46_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675669.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(4566..5798)
                     /gene="mdfA"
                     /locus_tag="QBF46_003532"
     CDS             complement(4566..5798)
                     /gene="mdfA"
                     /locus_tag="QBF46_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDQ6675670.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            6083..6679
                     /gene="ybjG"
                     /locus_tag="QBF46_003533"
     CDS             6083..6679
                     /gene="ybjG"
                     /locus_tag="QBF46_003533"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDQ6675671.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAVV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            6737..7495
                     /gene="deoR"
                     /locus_tag="QBF46_003534"
     CDS             6737..7495
                     /gene="deoR"
                     /locus_tag="QBF46_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDQ6675672.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(7542..8744)
                     /gene="dacC"
                     /locus_tag="QBF46_003535"
     CDS             complement(7542..8744)
                     /gene="dacC"
                     /locus_tag="QBF46_003535"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDQ6675673.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDIVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRVWDFVMMKFH
                     QWFGSWFS"
     gene            8991..9617
                     /gene="gstB"
                     /locus_tag="QBF46_003536"
     CDS             8991..9617
                     /gene="gstB"
                     /locus_tag="QBF46_003536"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDQ6675674.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(9614..10729)
                     /gene="yliI"
                     /locus_tag="QBF46_003537"
     CDS             complement(9614..10729)
                     /gene="yliI"
                     /locus_tag="QBF46_003537"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDQ6675675.1"
                     /translation="MHRPFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWSLAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(10840..11223)
                     /gene="bssR"
                     /locus_tag="QBF46_003538"
     CDS             complement(10840..11223)
                     /gene="bssR"
                     /locus_tag="QBF46_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDQ6675676.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            11436..12761
                     /gene="rimO"
                     /locus_tag="QBF46_003539"
     CDS             11436..12761
                     /gene="rimO"
                     /locus_tag="QBF46_003539"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDQ6675677.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(12808..14136)
                     /locus_tag="QBF46_003540"
     CDS             complement(12808..14136)
                     /locus_tag="QBF46_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDQ6675678.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQLPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(14144..16492)
                     /locus_tag="QBF46_003541"
     CDS             complement(14144..16492)
                     /locus_tag="QBF46_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDQ6675679.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRTNDKIVVYHQEQVNGPFVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLDASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         4298 a         4132 c         3849 g         4277 t
ORIGIN      
        1 tttgtcatgt tctttgttat taaacccact aactacctat gctagaaatt atccctgctc
       61 agggaaaaag ggaggtgttt ctcactgtac ctcagatggc aaattcgttt gcaatgatgg
      121 aactattagt aaatccaaaa aaatctgtac taaaaactca cgataacttt tgcttttata
      181 tctgcgtcta aaataaaaat gagccgcagg ttaaccgcaa aagttacatg ctcacatagc
      241 aaaaagaata gccaacttca tgatggcttc agtgagatgt atggtcgtag gatttcatac
      301 attgacactg gttatacata cagtaaaaat gctctctact ggagggcatt ttttatggca
      361 gtacgaaaac tcaccacagg gaaatggctt tgcgaatgtt accccgccgg acgtagtgga
      421 cgtcgtgtgc gtaaacaatt cgccaccaaa ggcgaagctc tggcttttga gcgccacacg
      481 atggaagaaa ccgaagcaaa gccctggctg ggtgaatcag tggatcgtcg aacactgaaa
      541 gacgtagttg agctatggtt caaactacat ggtaaatctc tgacagctgg gcagcatgtc
      601 tatgacaaat tgctgttgat ggttgacgct ctgggtaatc ctcttgcaac cgatctcacc
      661 tctaaaatgt ttgcccacta tcgagataaa cgcctgacag gcgagatcta cttcagcgag
      721 aaatggaaga aaggagcaag cccggtcacc attaacctgg agcaaagcta tctaagtagt
      781 gtttttagcg aactatcccg tctgggcgag tggtcgtatc cgaacccact ggagaacatg
      841 cgaaaattca ccatcgcaga aaaagagatg gcatggctta cccatgagca gattgttgaa
      901 ttgctggctg attgcaaacg tcaggaccca attctggcac tggtagttaa gatatgctta
      961 agcacaggcg cacgctggcg tgaagccgta aatcttaccc gctcacaggt gaccaaatac
     1021 cgaattacct ttgtcagaac gaaggggaag aaaaacagaa gcatccctat cagtaaagag
     1081 ctttacgaag agatcatggc gcttgatggg ttcaatttct ttacagacta ctattttcaa
     1141 tttttatccg tgatggaaaa aacgtctatc gtgctccctc gcggtcaact cacacacgtt
     1201 ctgcgccata cgtttgcggc gcacttcatg atgtcgggtg gaaacattct cgccttacaa
     1261 aaaattctcg gacaccacga tataaaaatg actatgcgtt acgcacatct ggcaccagat
     1321 catctggaaa cggcgctccg tttcaatcct ctggcaacgc tgccaagtgg cgacaaagtg
     1381 gcggcagcgg ttggcattac cccgtaataa ccaccactga ccaccaaccc aacttattgt
     1441 ttttaatcta acttattgtt ttcattaacc cgtttacata aatgggtttt ttgttatcta
     1501 aaacttatct actaccctgc aaccctctca accatcctca aaatctcctc gcgcgatagc
     1561 ggcttacggt cggtgacaaa atgcagcgtc atcccctcaa taaacgcatc aagcgcgcgg
     1621 gcggttccgg gttcaaacca ttgttcgagc gtttgctgac tgcgctgcat ccagttttgc
     1681 attaccgttt ttaatagcgg ttttcggcta gccagcgcgt agagctggta catcagctcc
     1741 atgttatccg gcgttgcaac ctgtgagctg tagatcatat cggtgatagc ctggcatgcg
     1801 cccggagcat cactaacatc gctaaaaaat gcctgatatt gccgggacat gatctcagta
     1861 aaactgctga acgcctccag taacaactca tcaattcctg aaaaatagta ggtcatcgat
     1921 cccaacggta ccccggcaag ggtagcaatt ttgcggtgcg taacagcatg tattccgtaa
     1981 agtttcaccg cctccagcgt ggcctggata attttttctc gccgttgcgg atcgttagcg
     2041 cgacgcatgt catttcctct ctctgcaatt gtgtacaaat gtacacaacc ttgctaatgt
     2101 tgtcttccct cttcttattt tgacgtgatc tatgaccgta aattcttcac gtaatgcatt
     2161 gaaacgccga acctgggcgc tgtttatgtt cttctttttg ccaggcctgt taatggcgtc
     2221 ctgggcaacc cgtacgcctg ctatccgcga tattctctct gtctcgatcg ctgaaatggg
     2281 cggtgttctc tttggtctgt cgatcggttc gatgagcggt attctctgct cggcgtggtt
     2341 agtgaaacgc tttgggacac gtaatgtcat cctggtcacg atgtcctgcg cattgatcgg
     2401 gatgatgata ttaagtctgg cactctggct gacatcgccc ctgctctttg ccgttggtct
     2461 cggcgtcttt ggggcaagtt ttggttctgc ggaagtggcg ataaacgttg aaggtgctgc
     2521 cgttgagcga gaaatgaata agacggtttt gccgatgatg cacggttttt atagcctggg
     2581 cacgctggca ggcgcaggtg tcgggatggc actgacggcc tttggcgttc cggcaacggt
     2641 gcacatttta ttggcggcgc tggtaggtat cgcacctatt tatatcgcca ttcaggcaat
     2701 ccctgacggt acgggcaaaa atgctgccga tggcacccag catggcgaaa aaggcgtacc
     2761 tttttatcgc gatatccagt tgctgctgat aggtgttgtg gtgctggcga tggcctttgc
     2821 cgaaggttct gccaacgact ggttaccctt attaatggtt gacggtcacg gttttagccc
     2881 tacttccggc tcgctgattt atgccggttt taccctgggg atgaccgttg gacgcttcac
     2941 cggcggttgg ttcatcgacc gttacagtcg cgttgccgtg gttcgggcca gtgcactaat
     3001 gggggcgttg ggtattgggc tgattatttt tgtcgatagc gcctgggtcg ctggggtgtc
     3061 tgttgtactc tggggactgg gtgcctcgct gggcttcccg ctgaccattt ctgccgccag
     3121 cgataccggc cccgatgcac caacccgcgt cagtgtggta gcaacgaccg gttatctggc
     3181 tttcctcgtc gggccgccgc tgctgggcta tctcggcgaa cattatggat tacgtagtgc
     3241 aatgctggtt gtactggcgc tggttattct cgcggctatt gtcgcgaaag ccgtcgccaa
     3301 acccgatacc aaaacgcaga cggcgatgga gaatagttga tgagcattaa attaattgcg
     3361 gtagacatgg atggtacttt cttaagcgat caaaaaacct ataaccgtga gcggtttatg
     3421 gctcagtatc agcaaatgaa agcacaagga attcgctttg tagtcgccag cgggaatcaa
     3481 tattatcagt tgatctcttt cttccctgaa attgctaatg aaattgcctt tgtggctgaa
     3541 aacggcggct gggtagtgag cgaaggcaaa gatgttttta atggcgagct gtcgaaggat
     3601 gcgtttgcta ctgtcgtgga acatttgctg acgcgcccgg aagtggaaat tattgcctgc
     3661 ggaaaaaata gtgcctatac actcaaaaaa tatgacgatg ccatgaaaac ggtggcggaa
     3721 atgtattatc accgtctgga atacgtcgat aactttgaca acttagagga tatcttcttt
     3781 aagtttggcc tgaatctttc cgatgaactg attccacaag tacaaaaagc attacatgag
     3841 gccatcggcg atattatggt gccggtccac accggcaacg gcagcatcga tctgattatc
     3901 cccggcgtac ataaagccaa tggccttcgc caactgcaga aattatgggg aatagacgac
     3961 agcgaagtgg tggtctttgg cgatggcggt aacgatattg agatgctgcg tcaggcaggc
     4021 tttagttttg caatggaaaa tgccggtagc gcggtcgtcg cagcggcaaa ataccgcgca
     4081 ggctccaata accgtgaagg cgtactggat gtgatcgata aagttcttaa acacgaagcg
     4141 ccatttgacc aataaaaacg gcagcacgcc cggcaacaca atgcctgaat gaaggctgca
     4201 taattcgcgg aaattaaccc gctgcattat ttcaggcgaa atgattatga aaaattgtct
     4261 actgttgggc gcgcttttaa tgggctttac tggtgtggcg atggcgcaaa gtgtcaccgt
     4321 cgatgtgccg agtggataca aagtggttgt ggttcctgat tcagtcagcg ttccacaagc
     4381 ggtcagcgtc gcgactgtgc cgcaaaccgt ctatgtggct cctgcgccag ctccggctta
     4441 ccgcccgcat ccttatgttc gccatcttgc cagcgtaggt gaagggatgg ttatcgaaca
     4501 ccagattgac gaccatcacc attaattcga gaatgcctga tcgcacaaag cagtcaggca
     4561 tttttttacc cttcgtgaga atttcccatc tgtttatctt ttaaaaagat aaccatcagc
     4621 gacagccaca aaattccgtt gacaaggttg aagagattaa acagtccgtt gcccccgttc
     4681 agccaggcat gtttgctgat ttcaatacca acggtaaaga tcagcatttg cagcattccc
     4741 atcgcggcag aaaccgtacc tttactcata tcgctggcaa acagggttaa tcgcaccagt
     4801 cccgcattcg ccagaccaat accgaaagca taaatactta acccggcagt catccataaa
     4861 tacgcgtgcg atgagataac cgttgccgca gcagcgacca atagaccaat cataatcggc
     4921 cagccgccca taataatcag cgaacgtacg gtgcggcgcg aggtcagacg cgctaacagc
     4981 aagttacccg caattaacgc cccgaaaata ggcacttgca gcaagccata ttcatagctg
     5041 ctcaactgct cgccggtaat gatgataatc ggcgactggg cgatccacgc cagcaacggc
     5101 agactaacga atcccagcgc cagcgccccc gccacaaagc ggccgttctt cagcaccagc
     5161 ttatagtcac gaccgagttc tttcagtgac agtttctcgc ctatacgcgt ggcggtttca
     5221 ggcatggctc gttgcagacc gaaaaaggag atcgctgcca atgcggcaaa caaaacaaac
     5281 atcccctccc agggcagcac atggatccac gccgcgccca ccagcggacc aagtagcgga
     5341 gcaatcagcg ccacgttcgc catcagcgcg gtgatcttga tacaaaccgc ctcttcgaag
     5401 gattcctgaa ttgcggcgta tcccacagcg ccaatgaaac agaggcttat gccctgcaag
     5461 aagcgcaaca gggtgaattg ttcaatgttt tgcgccagca atattgccag acaggtgacg
     5521 ataaaccaca ccactcccgc cagcatcacc ggacggcgac caatacgatc cgacagcggc
     5581 cccagcagcc attgtaaaaa catcccgccc gccagatacg cggtcatcga agtaggaacc
     5641 caatcaatgc ccgcctgata ttgttccacc acggccaaca taccgggttg aatcatatcg
     5701 ttgccgatat aggttgaaaa ttcgtaaagc accagacaga gagggaaaag taacgcctga
     5761 cgtccaagcc tggcaccgga agctaattta ttttgcatgc aatttcttcg ccaataataa
     5821 tcgcgcagag tttaataaaa gcgcagctaa cgagaaagcg aattttgtag ctgaaaccac
     5881 ggttaagcac attcttacat tattgcgagt atagctacgc tttctttaag ttttatttaa
     5941 cctatgcccg ttacaatcac ccaccgtaaa caggccgctt gagggaaata agacgatgcc
     6001 gctttaccca gtttaacctg cactttattc tcaacgactt gcctgtattg gctccctttt
     6061 aatcactttg cgtcgggaag ttatgctgga aaatttgaat ctctctctat tctctcttat
     6121 taacgcgacg ccagactcgg ctccgtggat gatctcgttg gcgattttta ttgctaaaga
     6181 tttgattacc gtggtgccgt tgctggccgt ggtactttgg ttgtgggggc ttacagcaca
     6241 acggcaactg gtgataaaaa tcgctatcgc gctggcggtc agcctgtttg tgtcctggac
     6301 gatgggacat ctttttccgc acgaccgacc ctttgtcgaa aatatcggct ataacttcct
     6361 gcatcatgcg gcggatgact cattcccaag cgatcacggt acggtgattt tcacctttgc
     6421 actggcattt ttatgctggc atcgcctgtg gtccggctca cttttaatgg tgctggccgt
     6481 cgtcattgcc tggtcgcgcg tttatcttgg cgtccactgg ccgctggata tgctcggtgg
     6541 attgctggca ggtatgattg gctgccttag tgcccagatt atctggcaag cgatggggca
     6601 taaactctat caacgtctgc aatcgtggta tcgcgtctgt tttgcattac cgatccgcaa
     6661 aggctgggtg cgtgactgat ctctgctaaa aagtgtagta ttgagcggct cgcttcaata
     6721 actattcaga gggattatgg aaacacgtcg cgaagagcgt atcgggcagc tgctgcaaga
     6781 attaaaacgc agcgataagt tacatcttaa agacgccgcc gccctgcttg gggtttcgga
     6841 gatgacgatt cgtcgcgatc tgaacaacca cagtgcgccc gtcgttttgc tcggcggcta
     6901 tattgttctg gaaccgcgca gtgccagcca ttacctgtta agcgatcaaa aatcccgcct
     6961 ggtggaagaa aaacgccggg cggcaaaact ggctgcgacg ctggtagaac ccgatcagac
     7021 cctctttttt gactgtggca ccaccacgcc gtggattatt gaagcgattg ataatgaaat
     7081 cccttttacc gccgtttgtt attcgctaaa tacctttctg gcgctgaaag agaaacccca
     7141 ttgccgcgcg tttctttgcg gtggtgaatt tcacgccagc aacgccattt tcaaacccat
     7201 cgattttcag caaacgctga ataatttttg cccggatatc gctttttatt ctgcggcggg
     7261 cgtgcatgtc agtaaaggcg ctacctgttt taatcttgaa gagttgccgg taaaacactg
     7321 ggccatgtcg atggcgcaaa agcatgtgct ggttgtcgac cacagtaaat ttggcaaggt
     7381 gcgtccggcg cgcatgggtg acctgaaacg ctttgatatt gtggtgagcg attgttgccc
     7441 ggaagatgag tatgtgaagt acgcgcagac gcagcgcatt aagttgatgt attaatgacg
     7501 tataaccgga tgacgtttcg cgccatccgg ttatcagaag attaagagaa ccagctgccg
     7561 aaccactgat ggaatttcat catcacgaaa tcccacaccc gaccaaagaa tccgccctct
     7621 tccacatttt ccatcacgat cagcggacgc tgctcaatgg atttaccgtt aagctggaaa
     7681 tcaatggtcc cgacaacctg accttttttc agcggtgcgg taagctgcgg ttccgttaac
     7741 gtataactcg ctttcaggtt tttcagctgc ccacgcggta tggtcactga gcccgcttca
     7801 cctgccccga gattcacttc gctcttatca ccaaaccaga cgcgctgagt cacaaaggtg
     7861 gcatcaggtt taattggcgt cacggtttca aagaagcgga aaccccaggt caataatttc
     7921 tcagactcat taaaacggat acggtcagtt ttcgccccca gcactacgga gattaaacgc
     7981 atatcaccct gggtagccga agcaaccaga ttatatcccg cacctgccgt ggttcctgtc
     8041 ttcatgccat caacattcag attgctgctc cacagcagac ggttacggtt aggctgacga
     8101 attttgttga aggtgaactc tttctcttta tgaatggcgt actcttccgg cacatcgtgg
     8161 atcaatgctt tacccagcaa tgccatatcg cgcgcggtac tgaactgccc cggcgcatcc
     8221 agaccgtgca ccgtctggaa ggtagtgttg gtcagaccca gttttttggc ataaccattc
     8281 atcaaaccaa taaatgactc ctggctcccg gcaacgtaat cggccagcgc aatacaggcg
     8341 tcattaccgg actggataat cacacctttg ttcaagtctg ccaccgaaac ctgatcgccc
     8401 ggtttgagga acattaccga tgaaccacgc agtgccggat ttcccgtcgc ccaggcatct
     8461 ttaccgaccg tcactatatc ggtgagttta atcttatcgg ccttaagcgc ctgcccaacc
     8521 acatagctgg tcatgatttt agtcaggctc gcgggatcca gtttctcatc cgcgttgcct
     8581 tctgccagca ctttaccgct ggcgtaatcc attaaaatcc atgcacgcgc atccacgctc
     8641 ggcgcttcaa cggtttgttc cgccgcgaat gccgttgggg caaaaaggaa taaaaatgca
     8701 gaacccgctg caagaccacg aaggagagag gagtattgcg tcataaatgc cacccgagta
     8761 tccattcaaa aaaaatacgt tatatcgccg tatgataagt tcggtgatta ataacgcaca
     8821 cacaaactta aagaaaccgt aagttggtaa agtttttaaa gtttatgcaa tatcctgccg
     8881 cttcgctgct tctggtgtca tttttttgcc agctgttgct taaaagttaa ctttacttca
     8941 ccatcggagt cctgttcagc ctgtggctgc ctgtcggaaa aggggtgagt atgattacgc
     9001 tgtggggtcg gaataattca actaacgtaa aaaaagtatt gctgacgctc gaagaactgg
     9061 aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg gataaatcac gatgctgatt
     9121 ttctggcgat gaaccctaac gggctggtgc cgttgttgcg tgacgacgaa agtgatctca
     9181 ttctttggga atcaaacgcc attgtccgct atctggcggc acagtacggg caaaaacgcc
     9241 tgtggatcga ctcaccggca cgtcgtgcgg aagcggaaaa atggatggac tgggcaaacc
     9301 agacgctcag taatgctcat cgcgggatcc tgatgggatt agtcagaaca ccaccggaag
     9361 agcgcgatca ggccgccatt gatgccagtt gcaaagagtg cgacgccctg tttgccctgc
     9421 tcgatgcgga actggcaaaa gtaaaatggt tctctggcga cgagtttggt gtgggcgata
     9481 tcgctatcgc accgttcatc tacaatttgt ttaacgttgg cctgacctgg acaccgcgtc
     9541 caaatctgca acgctggtat cagcaactca ctgaacgccc cgcagtgcgc aaagtggtga
     9601 tgattcccgt tagctaattg cgtgggctaa ctttaagtaa ttccccactg gactcgtcgg
     9661 tgagaacgta taaataaccg tcgggtccag tgcgaacatc acgaattcgc tgccctctgt
     9721 ccgttaaaat acggccatct tctgtcactt tgtcgccgtt gacgctcatc acaatgacat
     9781 ctttatcttt cagcgcgcca ataaataatt tttgctgcca ctgggggaat ttatcgctgt
     9841 tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca ggttgctcgg
     9901 tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag ttgattcccc
     9961 aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt tcatcaccac
    10021 cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc gccattcctt
    10081 gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc tttataaaag
    10141 gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc tgtaatttat
    10201 ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa agataacctt
    10261 taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt ggcatctggc
    10321 gaaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg ccataaccca
    10381 cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag atgcggcgag
    10441 actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg tgcgcccaaa
    10501 cgtccggcac tccggaaagc ggcgcagata atccttttcc tgcttgccag tgacgcaact
    10561 cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc agtgaccagg
    10621 gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc gcccagagag
    10681 cggaagaaag acaaataagg ggcacaagga aaaagaatgg tcgatgcata aggcctccgg
    10741 attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt ttacaaaaac
    10801 ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct caagcatggc
    10861 gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt caagcttatc
    10921 ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac atactgcggc
    10981 tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat ccagatgaag
    11041 attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt tatctcgtag
    11101 atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca cgtatgcggc
    11161 gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc tgtcaacgaa
    11221 catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc tattttttga
    11281 gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac atttccttcg
    11341 cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta gaatccctgc
    11401 cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact ccccagccga
    11461 aaatcggctt tgtttccctt ggctgtccga aaaaccttgt tgattcagag cgtattctca
    11521 ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg gacatggtga
    11581 tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa gccattggtg
    11641 aagcgttgaa tgaaaacggc aaggttattg tgaccggttg tctgggggca aaagaagatc
    11701 agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc tatgagcagg
    11761 ttctggagca cgttcatcac tacgtgccga aaccgaaaca caacccattc ctgagcctgg
    11821 tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa atttctgaag
    11881 gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac ctggtgagcc
    11941 gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc gttaaagaga
    12001 ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat cgtactggct
    12061 tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag ttatcgaaac
    12121 tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac gacgtcatcc
    12181 cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag cacgccagcc
    12241 cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg gcgcgcatca
    12301 aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt gtcggcttcc
    12361 ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa gcgcgtctgg
    12421 atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat gccctgcctg
    12481 accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag ctgcagcagc
    12541 agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg attatcgacg
    12601 aagtggacga agaaggcgca attggtcgca gcatggcaga tgcaccggaa atcgacggcg
    12661 cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt gtgaaagtcg
    12721 agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag gccgggcaaa
    12781 atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc tgccgttttt
    12841 cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg taggacattc
    12901 atttgaatgt gctacaccga tactggtact taaattaatg cttcccacct ggaaatgaac
    12961 gatttctttt agaatcgata gcaactcttc cagcgttcca cccgtataaa tcaccgcaaa
    13021 ttcgtcaccg ccgaagcggt agactttacc tttatctacc gggagctgtg atttaaggat
    13081 tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt cgttaatgtt
    13141 tttgaattta tcgacgtcaa acaggcacaa ataaccgcct ttttccggca gcgcttttaa
    13201 atcttcgtca aaggattttc tgcccattgc tttggtcagg tcgtccagga acgccgagtt
    13261 atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc catccagtac
    13321 gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat taatcactct
    13381 gcgtaccaga tagaggcaac tggcacccga tatcactgcc accaatgcgg caaagaaaat
    13441 aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacat acaatccggt
    13501 cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aaaatggcgt ttttagcatt
    13561 atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca ggttctcgct
    13621 gtccatcgcc agcgattttg acagtaaaga aaaacgctcc agaccaagca aaaatatcag
    13681 actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt caaaagagat
    13741 aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg cacaataatt
    13801 tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag gaccgacatc
    13861 gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa ccaacagctg
    13921 ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc gatattcagc
    13981 aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat acacccgttc
    14041 ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa aaatcagcag
    14101 actgactgta agtacgaact tattgattct ggacatacgt aaactactct tttactaatt
    14161 ttccactttt atcccaggcg gagaatggca tgggtttctg gtacagatac ccctgagcct
    14221 gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg ccttccgcaa
    14281 tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt tttcccgcag
    14341 taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga ttaatttcat
    14401 aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc cgtacccctt
    14461 gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg atcacggttt
    14521 cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg atggtggtaa
    14581 aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata tgcagtaaga
    14641 aatcatcctt cagcgacgca cgctgactaa aatgtctgaa ttcacccatt gccgttttaa
    14701 taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta atgaacttca
    14761 gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc gcgcccagaa
    14821 tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg ccaatttcca
    14881 ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg agtttcgcgt
    14941 catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc gccagtttca
    15001 gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg gcagacttat
    15061 ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc gctattttac
    15121 gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca ttgaatgtca
    15181 cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc ctgacgattt
    15241 tattgcgttc atcaacacca aacaccgctt ccacggcttc cagaccacac agtttgatga
    15301 tccccacagc cagatactga ttattgttgg actcaataat cgccatattt ttaataaaac
    15361 cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca atttcggcat
    15421 ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg ccttcgcgaa
    15481 ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta ttaaccagct
    15541 cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat accaccagca
    15601 acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg tccaataaat
    15661 taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact gctgatgtct
    15721 ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg ctattataaa
    15781 taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct tgtttcaaaa
    15841 atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac cgccagttta
    15901 aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg atacgtagcg
    15961 taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga ttccagctgt
    16021 tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa tcggcaaaaa
    16081 taacccgccc tgactcgtcg acaaacggtc catttacttg ttcctgatga taaacaacga
    16141 ttttatcatt cgtcctccgc gaaaagagat aatatttatt atcatcaagt accagtccca
    16201 ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga atctcctctg
    16261 tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca atatctttaa
    16321 agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt tttaccgcgc
    16381 gttccgatac cagttgatag ttaatgatga aaaaaagacc aataaatgac agtgctgcca
    16441 ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc atgttaatac
    16501 atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct tgctgc
//
LOCUS       DAOFQD010000107        16533 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.107, whole genome shotgun sequence.
ACCESSION   DAOFQD010000107 DAOFQD010000000
VERSION     DAOFQD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.107"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(140..292)
                     /locus_tag="QBF46_003542"
     CDS             complement(140..292)
                     /locus_tag="QBF46_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDQ6675680.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(557..1291)
                     /gene="cysH"
                     /locus_tag="QBF46_003543"
     CDS             complement(557..1291)
                     /gene="cysH"
                     /locus_tag="QBF46_003543"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDQ6675681.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRIAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWESGMSEEETRFFGLKRECGLHEG"
     gene            complement(1366..3078)
                     /gene="cysI"
                     /locus_tag="QBF46_003544"
     CDS             complement(1366..3078)
                     /gene="cysI"
                     /locus_tag="QBF46_003544"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDQ6675682.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDKWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESQKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3078..4877)
                     /gene="cysJ"
                     /locus_tag="QBF46_003545"
     CDS             complement(3078..4877)
                     /gene="cysJ"
                     /locus_tag="QBF46_003545"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDQ6675683.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQTAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDEPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAVDEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5196..5558
                     /gene="queD"
                     /locus_tag="QBF46_003546"
     CDS             5196..5558
                     /gene="queD"
                     /locus_tag="QBF46_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDQ6675684.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5636..6907
                     /locus_tag="QBF46_003547"
     CDS             5636..6907
                     /locus_tag="QBF46_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDQ6675685.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6898..7158
                     /locus_tag="QBF46_003548"
     CDS             6898..7158
                     /locus_tag="QBF46_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDQ6675686.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYQPSGFGITYRFG"
     gene            7175..7750
                     /locus_tag="QBF46_003549"
     CDS             7175..7750
                     /locus_tag="QBF46_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDQ6675687.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIYRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7898..8758)
                     /locus_tag="QBF46_003550"
     CDS             complement(7898..8758)
                     /locus_tag="QBF46_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDQ6675688.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8755..9534)
                     /locus_tag="QBF46_003551"
     CDS             complement(8755..9534)
                     /locus_tag="QBF46_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDQ6675689.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9512..10921)
                     /locus_tag="QBF46_003552"
     CDS             complement(9512..10921)
                     /locus_tag="QBF46_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6675690.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10943..12397)
                     /locus_tag="QBF46_003553"
     CDS             complement(10943..12397)
                     /locus_tag="QBF46_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDQ6675691.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12467..13252)
                     /locus_tag="QBF46_003554"
     CDS             complement(12467..13252)
                     /locus_tag="QBF46_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6675692.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13571..14848
                     /locus_tag="QBF46_003555"
     CDS             13571..14848
                     /locus_tag="QBF46_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6675693.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14875..16353
                     /locus_tag="QBF46_003556"
     CDS             14875..16353
                     /locus_tag="QBF46_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDQ6675694.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNRDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3945 a         4329 c         4159 g         4100 t
ORIGIN      
        1 taaattaatg attttcattt gaaattcata ataataaata gagactatat attcacataa
       61 aaaatataaa ttttaaaaaa gggtgcagga gaccaaacca tcacaattca gtacgcgggg
      121 ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc ttaaactcca
      181 taccacgttc tctgatactc aactcacaca acgagtcacc taccatcagc gtgaatccca
      241 gtactgtaca acacagtacg atgattgcca caagggcata ttttgtcagc atgtcttgcc
      301 tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgctgg cctcaggttg
      361 atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt aacgccagac
      421 agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg ttcacgttta
      481 aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca gaggcgttta
      541 atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag ccaaagaaac
      601 gtgtttcttc ttccgacatg ccagattccc atttacgggt tgtatgggta tcaccgaccg
      661 ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc aggtactgat
      721 aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca cgctgaattg
      781 ccagcaccgg taaattggcg cgactgccgg attgttcacg gcgcaggcca gcaaaccagg
      841 tttgcgcgtt aagttctttc agagcccggt tcatcggttc gactttgttg atgtcattgt
      901 acttttcaat gccttcaacg ccctgctccc acagttttcc gtagcgtgct tcctgccagg
      961 ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc gttaactcgt
     1021 caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc accggaatat
     1081 ccgggcgaat ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca aagctggaag
     1141 aaagtacata ttcaccgggc agattatcca gcgcccaggc tatgcggcct tcagcgtcca
     1201 gtttttccag ttcggcgtta gtttccgcca gcgccagaat gcgatctacc ttcggcagtt
     1261 cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc ttgcctgatg
     1321 cgacgctcac gcgttcttat caggcctaca agaccgggct gatggttaat cccacaaatc
     1381 gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc
     1441 ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat
     1501 ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc
     1561 gccaagatgc aggttgtagc gacccggcgc tttacccacc aggcccactt ccgccagcat
     1621 cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac
     1681 accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctccgc
     1741 ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccattgagt tttcacgctg
     1801 cggcgtgacg gcattcatta acccgctctc tttagcgatc ttctcgatct tcgctttctg
     1861 gctttcaggt acaccggcga tgatcagatt ctggttcgcc gtaatacgga aatcgccttt
     1921 gtggatcttc gcgatctcca gcaggccggt tttcagcgga cgccccggat aatcaaggat
     1981 tcggccattt tcgataaaca gcgtcaggtg ccatttgtca tcaatgcctt ttacccaacc
     2041 aatgcgatcg ccgcgccctg taaattcata cgggcggatc ggttcgaatt tgatccccgc
     2101 gcgacgttcc acttccgctt taaatgtctc aacacccacg cgctccagcg tgtatttggt
     2161 tttggcattt ttacgatcgg tacggttacc ccaatcgcgc tgagtcgtca cgacggcttc
     2221 cgccaccgcc agggtatgct ccagcggcag ataaccaaac tcgctcgccg tgcgggcgta
     2281 ggttttctta ttaccgtgtt cgatggaaag cccgccgccc accagcaggt taaagcctac
     2341 cagcttgccg ttttcggcga ttgccacgaa gttcatgtcg ttggcatgca gatcgatatc
     2401 gttctgcggc gggatcacta ccgtggtttt gaatttacgc ggcaaataag tctgaccgag
     2461 gatcggttct tcatcggtgg tggcgacttt ttcctgatcg agccagatct ccgcatacgc
     2521 gcgggtacgc ggcagcagat gttcagagat tttcttcgcc cattcatacg cttccgcgtg
     2581 tagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagcggt
     2641 cgccagcgca tcaagaccaa ccgagtgcag catctggtgc accggtttga cgttcttttt
     2701 cagaatgccg tggaactgaa acgtctggcg gttggtcagg cgaatgctgc catagatggt
     2761 gttttcaccg gcaaatttgt cgatcgcctg ccactgttta gtggtaatca ccccacccgg
     2821 cagacgacag cgaagcagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc
     2881 gcggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttatc
     2941 gcccttaaag ccgccggtca ggccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta
     3001 gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc
     3061 tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagttca
     3121 cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga
     3181 agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca
     3241 ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gtagtttgtc ttgtacgtaa
     3301 actttttctt tttgatcgcg cgaccaggcg agatcgatac gtgtcagcac gccctctttg
     3361 acgtagcgct gccactccac ctggtagagg aaatcttccg taaagtgcgg gttgccaaag
     3421 aacagccagt ttttacccgg tgcttcatcg acagcacgtt gctgcataaa ggcgcggaac
     3481 ggcgcgatac cggtgcctgg gccaatcata atcaccgggg tttctggatt ggctggtagg
     3541 cggaagttat cgttatgttc gataaatacg cggacttcgc cctcttcttc cacgcggtca
     3601 gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca
     3661 ccaacggtga cgtgtacttc gttctcgact tccgcctgcg aggaggcaat ggaatagaga
     3721 cgcggcgtca gcgggcgcag cagattaatt agcgcttcgg catcaagctg tgccggggag
     3781 aaacgcacca tgtcaacaat cggcgtcgtc gcggcgtaat gctgtaactt cgctttatcg
     3841 cccaccagcg gcagcagtgt ttcactgcgg gtaagcgtgg cgtaattctc aacaatgttg
     3901 gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg
     3961 ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc
     4021 agtgccggat cgttctgata ccagacgccc agcgcgtcac ccggctggta acgcaggccc
     4081 gagtcaccta agtcaatttc gatatggcga acgtcttttt cagagttacg cccggtaatt
     4141 ttctggttaa cagagaggct cgccaccagc ggctcgtctt tgctgtacgg gctggtgtgg
     4201 atttcattta ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat
     4261 ttaagcgcat caaccacacg agcgcgccac tcgctggcag cggtctggta ttcaacgtcg
     4321 gcatcgacac ggtcgagcag gcgttcaccg cccagttccg ccagcttgct gtcaaaatct
     4381 ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaatgcg
     4441 gtgttttcca gcttcggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct
     4501 tccggcggtt ccccttctcc ttgcgttgac gtcaccacga taagcagttt ttcgctggcg
     4561 atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt tgccgctaat
     4621 aaatcatcac gtaatgcttc agcaacacgg cgcgcattgc cggtttgcga ggcggagata
     4681 atagttatac ccggcatttc tgcggcggga gctggcgtcg ctgcaagcgc agcaggctgc
     4741 tgattgagca cgccccagaa atagccagaa acccaggcaa gctgggtggg agttaaatcg
     4801 gtcgtggccg cctgaaggcg taccagttgc tccgggttca acggaagcaa cgcggaaggt
     4861 gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt
     4921 cagcgcccat aaaacagaag agaaggtaag gttaacgggg tgaacggtgt ggattaaaga
     4981 cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa
     5041 caaaatagat taaccaacct aatgaaaaac aaatgaattt agccaatcat taagataaat
     5101 cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact
     5161 ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt
     5221 tcaccttcga agccgctcac cgcttaccac acgtcccgga agggcataaa tgtggtcgcc
     5281 tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg
     5341 gctggattat cgatttcgct gaactgaaag cagcgtttaa gccaacttat gagcgcctcg
     5401 atcaccatta tctcaatgat attccaggcc tggaaaaccc gaccagcgaa gttttagcaa
     5461 aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag
     5521 aaacctgcac cgctggttgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat
     5581 ttcccttaag taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga
     5641 agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg
     5701 ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc gctgaaatcc ccggcagcaa
     5761 aaatctttcc ggcgggcggt tatacaccca tgcactcgcg gaactcctcc ctcagtttca
     5821 tctgaccgcg cctcttgaac gatgcatcac tcacgaaagc ctttccctgt taacgccgga
     5881 tggcgcaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc
     5941 acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccctgg
     6001 tgcgacggtg gatgcactgt atgaagaaaa cggcagggtg tgtggtgtca tttgtggtga
     6061 cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga
     6121 gcgtcacggg ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt
     6181 gctgtcgctg gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc
     6241 agcgttgctg ttcagcggcg ggatctgtga tgacttaccc ggcggcgcat ttctttatac
     6301 taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcttccctta cgcaaagccg
     6361 tgttccggca agcgagctgc tgactcgctt taaagcgcat ccggcagtgc gcccgcttat
     6421 caaaaacacg gaatcactgg agtatggtgc gcatctggtg ccagaaggtg gcttgcacag
     6481 tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg
     6541 cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg ctgactggcg cgcaggcggc
     6601 ggcacaaacg ctgataagcg cctgccagca ccgcgagccg caaaatctgt ttccgcttta
     6661 tcatcacaac gtagagcgca gcctgctgtg ggatgttcta cagcgttatc agcatgttcc
     6721 ggcgcttttg caacgcccgg gatggtaccg tacgtggcct gcgttaatgc aggatatttc
     6781 ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt tattctggca
     6841 tcatttacgt cgtcacggcc tgtggcatct ggcgggcgat gttatcagga gtctgcgatg
     6901 tctgtagccc gtaatctctg gcgcgttgct gatgcgccgc acattgttcc ggctgactcc
     6961 gttgagcgcc agacggcaga acggttgatt aacgcctgtc cggcaggtct tttttcgctc
     7021 acaccggaag gtgacttacg tattgactat cgcagttgcc tggagtgtgg cacctgccgt
     7081 ttgctgtgcg acgaatcaac actacaacag tggcgctatc agccttccgg attcggcatc
     7141 acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc tgctccgcca
     7201 gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcaa tcgattctga
     7261 atgccaattt atttccgtgt tgtacggcaa tatctgcacc atcagtaata tcgttaaaaa
     7321 gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa
     7381 taaagaagtc gttattcagt ttttgaaact ggtgaccgag gcggacggca ttatcagtac
     7441 caaagcctca atgttgaaag cggcaagagc ggaaggtttt ttctgtattt atcgcctgtt
     7501 tattgttgat tcaatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga
     7561 ttgtattgag atcctgccag gctgtatgcc caaagtgctg ggctgggtga cagagaaaat
     7621 ccgccaaccg ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat
     7681 taacgcgggt gtcgtggcgc tttccaccac gaataccggg gtctggacgt tagcgaaaaa
     7741 attactttga cgggataatc gtaaccaatt gaatttggtt tgatttgtag gccgcacgcc
     7801 acatccgaca ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaagtccc
     7861 caggccagaa ccgtaggcag gatcatccgg caacacgtta ttggcaatct gcgtgaatgt
     7921 ttgtcaccag tgcctccagc accactttcc agtcatcaac cacgccaaca tctgcctgtg
     7981 aaaataccgc agcgctggcg tcatgattaa tcgccaccac aaacttgcta ttgcgtacgc
     8041 ccgccattaa cgctgctgcg ccagaagcgc caacaacaat acacacctca ggtgccagca
     8101 gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc actcgcgccc
     8161 gactgtagcc cacttcggct cccagctttt ctgccagcat agcgatttcc tggttatctg
     8221 cctctccgcc ctgccccacc accagcaccc gtctggcctc agctaatggg tcgcgagtaa
     8281 catttttcag atcctctgtg ctaaccagcc agtccggaag cgcacccggg acaatgttca
     8341 gttgttgcat tccagagggc aacgtggcgt ttttcgctgc tcccgcctga cgcgccaggg
     8401 aaaggcatag cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt ccccagtggg
     8461 atttgcgcac gcttacggtt ggaatatcta acgaagtcac ctggcagatg cttgcaccat
     8521 gtaaacgcca ggccagccgg gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa
     8581 caacgtccgc aggcgttcgc tgccactgtt ccaccaacgc atccagcacc tgttccgcca
     8641 ctacaggctg aggttcaatc tgccagtgcg ccagggtaca accgctgaaa tcctgcgccg
     8701 ccagccagct tgctattgcg gcattctcct gatttattgt cacaattgcg atattcatgg
     8761 ctgcatcctt tggcgtaaat agtcctgcca tagcttttga gctttttctg ctaccgtttg
     8821 cccatcaatc agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca gacactgcat
     8881 cgctggcatt tcggcagcaa cagttttacg gataatttct gctttccccg cagccattcg
     8941 ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc
     9001 aggcaagcga acccggcaac aacgcaaccc atgttcagta cgctgttcga gggtgataaa
     9061 cagcgcgtcg agagtgaaac gctccacctg ggtgaagcag ggccagccca gcatttccgc
     9121 cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag
     9181 atccagcgga ttctgatgct gccattcggc aatatggcga gcgacaaatt ccggcgcaaa
     9241 gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata
     9301 gcgcagccag tgcaatgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt
     9361 accgtttttt ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag caccgagtaa
     9421 acttcgcagt agcgaaatat ccggtccgct tttaccctga gccgccgcct gccactcttt
     9481 ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac
     9541 ctcctacatt tcccgccgcc accagcggga gggctttagt ttctggtgcc catagccagg
     9601 tcacaacaaa accaaccaac aacactgtcg ccaggagcaa taaggtgact tgcattcccc
     9661 actgcgccag cacccacggc agcaggccag tacttaccgc cgcgccaagt cgactcatgg
     9721 cggtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa
     9781 tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg
     9841 ttaatgaact gccggaaggc aaacaggcca ttactaccag cgttgccgcc agcagcaaaa
     9901 aacttcccag caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg
     9961 cgcccacaat taacaacgca ttaagcatca ggctggcagt cagcgcatct tccagaccaa
    10021 tagtctgggc gatagttggc agccaggtat aaatcacaaa ccatgggatt acgaggcaga
    10081 caaagaagac gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac aaggttttga
    10141 tgtgtttatg ggtcgccgtt accacttcat cgcccagtaa aacatgggga ccaaaatagc
    10201 gatgcacgat agcgtgagct tctgcaaaac gcccctggcg cagtagccag cgtggtgatt
    10261 ctggcgttcc ccagcgtaat aacgtaatca acaacgcggg cagagctgcc gatgccagta
    10321 gccagcgcca ggcctccggg ttttcggaaa taaagtgatg tccggcaata cttgccagca
    10381 catagcctac ggtccacacc acgctgaatg cgcccagcaa aataccgcga tggcggcgcg
    10441 gggaaaattc agccagcaag gtgtgaccta ctgaataatc gcctcccaga ccaatgccaa
    10501 tcaaaatgcg cagtccaata agatgctctg gcgtggtggc aaaaaattgt aaaaacgaag
    10561 caagcgtaat cagcaaaaag ctgaaggtga agattttttg ccgaccaata tggtcggaga
    10621 tccacccaag aaccaggctg ccaaggaaca aaccaaggag cgccgagccg ccgatcattc
    10681 ccgccataaa cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg taaccaatga
    10741 cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca atgcggcagt
    10801 gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc tttacccctt
    10861 cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa cgccccgtca
    10921 tcgcggaacg ctgcctgaca cattattttt caatcggata gatagtaccc gtattcataa
    10981 tgccattagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt
    11041 ccagtttgct ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc atcgaaccgc
    11101 cgaggcgaat ggtttcttca cagatgatct tgttgagcgg attgtggtac ttgtcgattt
    11161 cctcttccgg cttacagtca acgacgttgt aatcgtagac gaagtacatg ttggtgccgt
    11221 tctgatagct atgagaggaa tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact
    11281 cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc cagcagccgg
    11341 acacttcagt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcggcagcca
    11401 ctttatccgg tccccagttc aggttgttaa accaggtttc gatcagcttg ctgtccacgc
    11461 gctggcattg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc gccttcgcaa
    11521 tgcgagggtt accttcagcc ataaagatca gcacgcattt tccgtcggca aaatgggtga
    11581 agtgttgggt gccatcttca gcgtcataca aacgagcgat cgacggacga tacccttcca
    11641 ccatgatttc acgcaggatg ttgaagccgg ttttcatgtc ttccaggata tagccgtaga
    11701 agaggttgtt ttccggggtg aatttaaaga ttttcactgt tacttcagtg atatagcaaa
    11761 gtgcaccttc gttaccgatg atgatgtggc gaatgtccgg gccagccgcg cggcgtggca
    11821 cgtttttaat acgtgtcacg gtgccatcag ccaatactgc ttccagacca acgaccatat
    11881 cttcgattgc gccgtagagt gtggagaact gcccgatact gcgggttgct accaggccgc
    11941 ccatctgcgc cagcggcttt gactgcggag aatgccccgt ggtgtaacct ttttcacgca
    12001 acgcgttttc cagcacttcc agcggaacac cacattgcgc cgtggcctgc atattctcaa
    12061 tatcaatatt aatgatttga ttcatggcgg agccgtcgag caccaccgag ttttccacaa
    12121 cggtttccag cccaccttcg gtagcggaag caccggtacg cggcacaccg ttaattttgt
    12181 gcgcattcat aaaattcagc acacgggaga cttgttctgt ggaaccgagt tttacgaccg
    12241 ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga aaacggtcaa
    12301 tactgttctt ctttaatact gtttcatcgg taattacgcg atctgcacca acaatttcct
    12361 ttagctggtc gacaatcgct gcgcgagata aagacatagt aattccttcc tgataataaa
    12421 aatggaaaaa tcattaataa gagaaaacag aaaccaccgt tatggattag cgcactaaat
    12481 aaccgccatc gaccactaat aaatgcccat tgacataatt cgatgcctga cttgcgagaa
    12541 acacggctgc acccattaaa tcctgagtat cgccccaacg gtttgccgga atatgatcaa
    12601 gaacgcgctg attggtttct ggattactgc gtgtcgccag cgtaatatct gttgcataat
    12661 agccaggggc gataccattt acctgaatat tatattgacc taattcatca caataagctt
    12721 tggtgaaccc ggcaagagca tgtttagtgg cagaatatgc gggtgaccat tgtccaccta
    12781 agtaagagaa caatgaacag atattaataa ttttaccgct tttctgcggg atcataattt
    12841 ttgccgcttc atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc atcggatccc
    12901 agtcggcacg accgaagtcc agcaccttat tcagcttaca aataccggca ttgtttacca
    12961 gaatatcaac tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt
    13021 ctgcggtgat atccacctgc atgaagtcca cctcaacacc ctgtttttca atcatttcct
    13081 ttgtttcgcc gttatctttg acgaaactag gaataaagat atttgcgcca gctttggcca
    13141 acgccatggc aaatgcctgg cctaaaccgc tattcccacc ggtaacaatt gcggttttac
    13201 ctttcagtga gaaaaaatcc attgagaacg cattgagaga ttcgattgac atagttagct
    13261 ccaatttccg taaggcaaaa aaaaagaaag gtaacttccc ccgcgagagg gaagttacct
    13321 ttctcattat ctctggtaac tggagataat ttttagcata acggcataac tgctaatgtg
    13381 atcgtaatca cagtgtgata ttcgtattgt caaatagttt taaaaagcag aagcgttatt
    13441 actctcacac aacacctctg ctttccatgt tatttcttat tgcaattact agagacctga
    13501 gaaaagtaat ccacctgaaa gggcggctta cttttctctt ttttttgtct attacaaatg
    13561 ggaatacgat atgcaacaca actcatatcg ccgttggata accctcgcga taattagttt
    13621 tagcggcggc gttagtttcg acctggctta tttacgttat atttatcaaa ttcccatggc
    13681 gaaatttatg ggattcagca ataccgagat aggtttaata atgagtacct ttggtattgc
    13741 ggctattatt ctttatgccc ccagcggcgt tattgccgat aaattttcac accgcaaaat
    13801 gattacttcc gcgatgatca ttaccggatt actgggtctg ttaatggcaa cgtatccacc
    13861 gctctgggta atgctctgta ttcaggtcgc ctttgcgata acaacgattt taatgctgtg
    13921 gtcggtgtcg attaaagccg cctcgttgct gggcgatcat agcgagcaag ggaaaattat
    13981 gggctggatg gaagggctgc gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg
    14041 ggtcttttct cgctttgcac cggatgacag caccagcctg aaaacggtca ttatcatcta
    14101 tagtgtggtt tacatcttgt tggggattct gtgctggttt tttgttagcg ataacaacaa
    14161 cctgcgcagt gccaataacg aagaaaaaca gtcattccag cttagcgaca tcctggccgt
    14221 tttgcgcatc agcaccacct ggtattgcag catggtgatt tttggtgtct tcaccatcta
    14281 cgccattctg agttactcca ccaactatct gaccgaaatg tacggcatgt cgctggtggc
    14341 ggcgagctac atggggattg tgatcaacaa aatcttccgc gcgctgtgcg gcccacttgg
    14401 cggcattatc accacctaca gtaaagtgaa atcccctacc cgcgtgattc aaatcctttc
    14461 catcatcggt ttgctggcgt tgacagccct gctcgtcacg aactctaacc cgcaatcggt
    14521 cgcgatgggg attggcctga ttttactgct gggattcacc tgttacgcct cacgcgggct
    14581 gtactgggcc tgccccggcg aagcgagaac accgtcttac attatgggca ccacggtagg
    14641 tatttgttcg gtgattggat tcctgccgga tgtcttcgtt tacccgatta tcggccactg
    14701 gcaagacacc ctgcccgctg cagaagccta ccgcaatatg tggctgatgg gcatggcggc
    14761 gctcggcatg gtgattgtct ttaccttttt gctgttccaa aaaattcgta ctgctgatag
    14821 cgcccccgca atggctagca gcaagtaagc caggaaaatt acaggagaaa aatcatgtcg
    14881 aaaaaataca tcatagggat tgatggcgga agtcagagca caaaagtggt gatgtacgat
    14941 ctggaaggta atgtggtttg cgaaggtaaa ggcttattac agccgatgca cacgccagat
    15001 gccgatactg cagaacatcc tgacgacgat ttatgggcat cattatgttt tgccggtcac
    15061 gatttgatga gtcagtttgc cgggaataaa gaagatattg tcggtattgg tctgggatcc
    15121 atccgttgct gccgtgcgtt attgaaagcc gatggcacgc cagctgcgcc gttgattagc
    15181 tggcaggatg cacgcgttac acgcccttac gaacatacga atcctgacgt ggcgtatgtc
    15241 acctcttttt cgggttatct gacgcatcgc ttaaccggcg agtttaaaga caatatcgcc
    15301 aactattttg gtcagtggcc ggtggattat aagagctggg catggagcga agatgctgcg
    15361 gtaatggata agtttaatat cccccgtcat atgctgtttg atgtgcaaat gcctggcacc
    15421 gtcctcggac atatcacacc acaagccgca ctggcgacac atttcccggc aggactgccg
    15481 gttgtttgta ccaccagtga taaaccggta gaagctctgg gggctggatt actggatgat
    15541 gaaactgcgg taatttctct tggcacttac atcgcattga tgatgaacgg caaagcactg
    15601 ccgaaagatc cggtggcgta ctggccgatt atgtcttcta ttccgcaaac attgctgtat
    15661 gaaggttacg gtattcgcaa aggtatgtgg acggtgagct ggctgcgtga catgttaggc
    15721 gagtcgttaa ttcaggatgc cagggcgcag gatctttcac cggaagattt actcaacaag
    15781 aaagcctcta gtgtgccacc tggctgtaat gggctgatga cggtgctgga ctggctgacc
    15841 aatccgtggg aaccgtacaa acgcgggatt atgatcggct ttgattccag catggattac
    15901 gcatggatat atcgttcgat attggaaagt gtggcgctaa cgctgaagaa caattacgac
    15961 aatatgtgta atgaaatgaa tcactttgcg aagcatgtga tcattactgg cggcggttcg
    16021 aacagagatc tgtttatgca aatttttgcc gacgtgttca accttccggc acgccgtaac
    16081 gccattaacg gttgtgcaag tctgggagca gcgattaata cagcggtagg tctggggcta
    16141 tacccggatt acgcaacggc tgttgataag atggttcgcg tgaaagatat ctttataccg
    16201 attgagagca atgccaaacg ctacgacgcg atgaataaag gcattttcaa agacctaacc
    16261 aaacatactg atgtgatcct gaaaaaatcg tatgaagtga tgcatgggga attggggaat
    16321 gtggattcga tccagagctg gtcgaatgcg taagtgagga aggccgggcg ggaaactgcc
    16381 cggcctgaac atacctgaat ggttatcccc gctaacgcgg ggaacataag ttcgacaact
    16441 taaaaaatga taacaagtcc ggtttatccc cgctgatgca gggaacataa taaaaacttt
    16501 aactagttcg gtttatccct gtttttacaa gga
//
LOCUS       DAOFQD010000108        16456 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.108, whole genome shotgun sequence.
ACCESSION   DAOFQD010000108 DAOFQD010000000
VERSION     DAOFQD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.108"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            54..509
                     /locus_tag="QBF46_003557"
     CDS             54..509
                     /locus_tag="QBF46_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDQ6675695.1"
                     /translation="MSMNSQPELKLSTRTEQLASSRDAAMQKFLDGMTLIAEASAICG
                     FSLFNSKIMAPNAFGLPASLAASIEEGRQQIDRKTWNNLFEETGIDRFWNHNQRAEFR
                     ESLRNAPPIASLTVIRSTLRQAVAMRSITLAEGFVDLLCQLDRRYKTNA"
     gene            678..866
                     /locus_tag="QBF46_003558"
     CDS             678..866
                     /locus_tag="QBF46_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675696.1"
                     /translation="MYDRLAVLRKTDFTGDITDPKGWKFRLFGNGNVHISVECESLHN
                     ALNDLISIYFANQIPAKG"
     gene            872..2089
                     /locus_tag="QBF46_003559"
     CDS             872..2089
                     /locus_tag="QBF46_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675697.1"
                     /translation="MTDNVNLMNGDEQTASSLSEETSKTPPLSPELLKNSPYSSIAKV
                     KYFTEIEQICLDKSISTENLDQFFKAHWLRDKMGGSFARAQEMLAAYKQYVDEVPEEA
                     RAIEIPDQIKDAFSDFTAFITWYFRLSYTAIQSDSVKIAKAEITQLRQRNAEILEELS
                     QSKEQATVLNNEKVNLINLLEQQRELSSKLEDSLQEAEETLAGTQSELQHAQNEIQLL
                     QQTVGTLNQQLSERKQELASQQEYQKQLNDENKAQQVELTALKSQNDHLQRTVSDLKV
                     SVSQLEQDLSSVQTHSSELSSSLAEKDTTLTLVRSELSTAKGENDQLRAEAQRLADES
                     LVAKKVQTDQTEELQQLRNQMISMEATLNAEKTIAESLRGTIKQLTEAMTGAVAIKPK
                     SSGASKPRNKKTT"
     gene            complement(2144..2347)
                     /locus_tag="QBF46_003560"
     CDS             complement(2144..2347)
                     /locus_tag="QBF46_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675698.1"
                     /translation="MAYILSMSEQVKLKAFLAAVLDDYKLGAISQQQAVGGITSLVDA
                     IEISDSGKISLMLSEGRKLLRTG"
     gene            complement(2385..3680)
                     /locus_tag="QBF46_003561"
     CDS             complement(2385..3680)
                     /locus_tag="QBF46_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1173 family protein"
                     /protein_id="HDQ6675699.1"
                     /translation="MGQKFAVFIAYDDEPNTKRYSAEFQTQDEYVKGWQSALKKAHHT
                     SGQKSVISCGCRGKGAKRLYVRSLPNSDTFILIKAANTGTEHDPSCVFFDLDARHTGL
                     KGYASNVVRINNEGTMSIRLGIGMTEKDPPEKSEVPSLPQIQRPEGGQASMTLSGLLS
                     LLWTEAGLNVWYPNMAGKRNDSLVRYRMLEAAKQIRSGRACIGDHLFIGVADSKSKVA
                     SEQVQLLSSAELSDKRLLLLSVLPRYDAEKHEKPLKFLPMRNFGGMPLTFFNSDGHWD
                     SVKRRFPLEYAAWKNGGKVVVFALTSPVSVTSRGISARAHQIVLMLVSDNWIPLDSSY
                     EAIVSRKLDAEHRHYVKPMRYDASASDVFPDFYLLDTRSDKPFPMEVFGMSTPAYLAR
                     KELKKAYYNQEYGAYGWWHWDATEKTEYQELPHFPEARR"
     gene            complement(3705..3875)
                     /locus_tag="QBF46_003562"
     CDS             complement(3705..3875)
                     /locus_tag="QBF46_003562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675700.1"
                     /translation="MMTYFDSAEDLTISKQRALQELAKHGVVASDIDVFFSELGEREE
                     YNAQEVLIWLGY"
     gene            complement(4004..5404)
                     /locus_tag="QBF46_003563"
     CDS             complement(4004..5404)
                     /locus_tag="QBF46_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDQ6675701.1"
                     /translation="MTALLNDETPSSIHKLLVQVASIYDVKDLAAQLNAATGSDWSRA
                     SLIRQIKGSVNECRITQEEYHYLRSLLPSRPADYDQKFFRFIDLFAGIGGLRSGFDAI
                     GGKCVFTSEWNQFSRRTYSANWYCDETEHYFNSDIRDITLSNLPDVSDDQAYASIDAS
                     IPDHDVLLAGFPCQPFSIAGVSKKNSLGRKHGFECDTQGTLFFDVARIIRAKQPAIFV
                     LENVKNLKSHDKGNTFRIIMKALDELGYDVADCDAHGSKDPKIIDGKNFRPQHRERIV
                     LVGFRRDLKLHDGFSLSGVSALYPSRRPTLGELLDAEVDSRYILSPKLWEYLYNYAKK
                     HQAKGNGFGFGLVNPLDKSVVCRTLSARYYRDGSEILLDRGWDHSIGEKDFNDPVNMA
                     RRPRRLTPRECARLMGFEQPGKVTFRIPVSDTQAYRQFGNSVIVDVFAAVASLLRSRI
                     EIAVAARLKGEFEAAS"
     gene            5655..6170
                     /locus_tag="QBF46_003564"
     CDS             5655..6170
                     /locus_tag="QBF46_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDQ6675702.1"
                     /translation="MTLLKKLLLPTLIVLFPAATLAAQSSFEAKVVKIIDGDTITALD
                     GQNTSIKIRLYGIDAPESKQAFGQKSKQALSAAIAAQNITVIDHGPDIYGRMLGTIWL
                     DGYDINASMVDSGYAWVYRFDGNAIVPNYLKFEASAQKAVKGLWVDPNPVAPWEWRQQ
                     NQKPQKTKSRG"
     gene            6176..7099
                     /locus_tag="QBF46_003565"
     CDS             6176..7099
                     /locus_tag="QBF46_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675703.1"
                     /translation="MTRQHPLLKIALNSDGHLSWPILPGETHRCPTCLRHVTTGPDGD
                     LVHSMEEGGNVCIPSASVVISKAIIEMLSAGERLYVNPIKNGNKTLAPSFIFLHTDQQ
                     LRPFINSDYQPAGASWRSDKGFRLGLFFMNERASKINKDQFDFIAVIDPLTFQSEFDA
                     TWSRGEFDDPLKALRYSIVSENLSSVWVKWPVKNLQPRKETVMADYWYDYYSPHSPAQ
                     QAACTATVTGIDGNVSGETIYMLQIALERSTVEYQLVKSLGVILLLDNTGNLVSEAQP
                     HFDIIIKACISGVRDFVLQNPRTLGFENKVA"
     gene            7155..7934
                     /locus_tag="QBF46_003566"
     CDS             7155..7934
                     /locus_tag="QBF46_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HDQ6675704.1"
                     /translation="MVQVLNTTGLNYQLEKTITEAEERIILISPYLKLSNRIKELIED
                     KNRLKVDIRIVYGKSELNSKEYEWLTNLPFVRLSFCKNLHAKLYANENQCIVCSLNLY
                     DFSQINNHEIGVLIIKEQEKDAYTSSLNEAQRLIRISTDNIQPLTTDKETTGSKESEK
                     TNKESSDFTSAFHEEPLTNQNGLITDLNTNSKLTSTNLAKNHKTDLLSLYKKLIEAGY
                     LMDVEGKYILTDAGIAAGAEAKPNRYKKGENYFLWPDNLAL"
     gene            8282..8581
                     /locus_tag="QBF46_003567"
     CDS             8282..8581
                     /locus_tag="QBF46_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675705.1"
                     /translation="MENLDVVSAVDFYLEKAFYLFGFIMLGMAWLTFRAGSTYQHFKN
                     ADKLVSEGRGSVSSGKEPEMIRRLKFQTLIWYIANCVVLALSTIAFGYLFYLLHS"
     gene            complement(8831..9463)
                     /locus_tag="QBF46_003568"
                     /pseudo
     CDS             complement(8831..9463)
                     /locus_tag="QBF46_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
     gene            9572..10780
                     /locus_tag="QBF46_003569"
     CDS             9572..10780
                     /locus_tag="QBF46_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HDQ6675706.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
                     DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
                     AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNL
                     FTHGYVLGKL"
     gene            complement(10796..10915)
                     /locus_tag="QBF46_003570"
                     /pseudo
     CDS             complement(10796..10915)
                     /locus_tag="QBF46_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(11146..12351)
                     /gene="gltS"
                     /locus_tag="QBF46_003571"
     CDS             complement(11146..12351)
                     /gene="gltS"
                     /locus_tag="QBF46_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDQ6675707.1"
                     /translation="MILDASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIV
                     AIVLLIIDKTSGYSFTFDASLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTI
                     LIAIQNTVGMSMAVMMNESPFIGLIAGSITLTGGYGNAGAWGPILADKYGVTGAVELA
                     MACATLGLVLGGLVGGPVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVI
                     ETISMLIICIVVGGYISALFKDTFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVD
                     VLGSVALSLFLAMALMSLKFGQLASMAGPVLIIIAVQTVVMVLFACFVTFKMMGKDYD
                     AVVISAGHCGFGMGATPTAIANMQTVTKAFGPSHKAFLVVPMVGAFIVDISNSILIKI
                     FIEIGTYFT"
     gene            12795..13115
                     /locus_tag="QBF46_003572"
     CDS             12795..13115
                     /locus_tag="QBF46_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDQ6675708.1"
                     /translation="MIAVIFEVQIQPDQQTRYLTLAEELRPLLSHVAGFISIERFQSL
                     ATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKD
                     KDNV"
     gene            13108..13494
                     /locus_tag="QBF46_003573"
     CDS             13108..13494
                     /locus_tag="QBF46_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HDQ6675709.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            13502..14188
                     /locus_tag="QBF46_003574"
     CDS             13502..14188
                     /locus_tag="QBF46_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDQ6675710.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHARVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(14166..14789)
                     /gene="tetR(B)"
                     /locus_tag="QBF46_003575"
     CDS             complement(14166..14789)
                     /gene="tetR(B)"
                     /locus_tag="QBF46_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDQ6675711.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            14871..16076
                     /gene="tet(B)"
                     /locus_tag="QBF46_003576"
     CDS             14871..16076
                     /gene="tet(B)"
                     /locus_tag="QBF46_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDQ6675712.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(16189..>16456)
                     /locus_tag="QBF46_003577"
     CDS             complement(16189..>16456)
                     /locus_tag="QBF46_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6675713.1"
                     /translation="IQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDD
                     VLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT         4844 a         3497 c         3512 g         4603 t
ORIGIN      
        1 tggataggcc agtgaaaatc tctcagccaa aacagtggga aaaggatcgt gaaatgagca
       61 tgaattcgca gccggaactg aagttgagta cccgtaccga acaactcgcc agtagtcgtg
      121 atgccgctat gcaaaaattt ctggacggca tgacgctgat tgccgaagcc tcagcaattt
      181 gcgggttttc actgtttaac agcaaaatta tggcccctaa tgcttttggc cttcctgcgt
      241 ccctggccgc aagcatcgag gaagggcgac agcaaataga ccgtaagaca tggaacaatc
      301 tttttgaaga aacgggtatt gaccgattct ggaatcacaa ccagcgggcg gagttccgtg
      361 agtctctccg gaacgctccg ccaattgcgt cgttaaccgt aatccgcagt acattgcgcc
      421 aggcagttgc catgcgcagc attacgcttg ctgaagggtt tgttgatctg ctatgccagc
      481 ttgatcgccg gtataaaact aatgcctagc aatttgtgat gccaaaaaaa ctggtactac
      541 gcggcatttt tcccgactcg aacatgatga gatataatgg cttttctcag gataatctct
      601 tctatttaaa cgactttgag aatgtcgttt gcatctgctc caatgcagca acaccgcctg
      661 tcggcagtgg aatgaacatg tacgatcggc ttgctgtctt acggaaaacc gatttcacgg
      721 gtgacattac cgacccgaag ggctggaagt tccgattgtt cggaaatggt aacgtccata
      781 tctcagttga gtgtgaaagc ctgcataacg cactgaatga cctgattagc atttattttg
      841 ccaaccagat cccggccaaa ggataagaat gatgactgac aatgtaaatt tgatgaacgg
      901 ggatgaacaa actgcgtcca gtttaagtga agagacttca aaaacgcccc ctctaagccc
      961 cgagttactg aagaattccc cttactcgtc aatagctaag gttaaatatt tcaccgagat
     1021 tgaacaaatc tgcctcgata agagcatcag caccgaaaac ctcgaccagt ttttcaaagc
     1081 ccactggctg cgggataaaa tgggggggtc ttttgcccgg gctcaggaga tgctggcggc
     1141 ctacaagcaa tacgtggacg aggtgcccga ggaagcacgc gcgattgaaa tccctgacca
     1201 gattaaagat gcattcagtg attttactgc gtttatcacc tggtacttcc gcttaagcta
     1261 tacggctatc cagagtgata gcgtgaaaat tgcgaaggca gaaattactc agttacggca
     1321 gcggaatgct gaaattctgg aagaactgtc acagtcgaaa gaacaggcta cagtcctgaa
     1381 taacgaaaaa gttaacctga ttaacctgct ggagcagcag cgtgaactct ccagtaaatt
     1441 agaagattca cttcaggaag cagaagaaac tctggcagga actcaaagtg aattacagca
     1501 tgcccaaaac gagatccagc ttttacagca gacagtaggg acactgaacc agcaactgag
     1561 tgagcgcaaa caggaactgg cctcgcagca ggagtatcag aagcagctga atgatgagaa
     1621 taaagcgcag caggtagagc tgacagccct caaaagccag aatgatcact tacaacggac
     1681 cgtgtctgat ttaaaagtca gtgtcagtca gcttgaacag gacctttcct cagtacagac
     1741 ccattcctct gaattgagct catctttagc cgaaaaggac acgaccctga cgctcgtgcg
     1801 gtcggagctc tcaacagcta aaggggagaa tgaccaacta cgagctgaag cacaacgctt
     1861 agctgatgag agcctggttg ccaaaaaagt gcaaacagat cagacagaag agcttcaaca
     1921 gctcaggaat cagatgattt ccatggaagc gacactcaat gcggaaaaga ccattgccga
     1981 gtctttacga ggaacaatca agcagcttac cgaagctatg accggcgcag ttgccatcaa
     2041 acctaaatcc tctggggcat caaaaccgcg aaacaaaaag acgacataac tgctcccgcc
     2101 aggaatttgc aatagactta aattcctggc gtccacaggc tctttatccc gttcgcagga
     2161 gtttacgtcc ttccgacagc attaggctga ttttccccga atcactgatc tcaatagcat
     2221 caaccaggct agtgatcccg ccaacagctt gctgttgtga aatagctccc agtttgtagt
     2281 catcgagtac tgccgccaaa aacgccttca gtttaacctg ctcactcatc gaaagtatgt
     2341 acgccatttt tagcccaatt ttaaggttgt gattcgggtt aattttatcg tctagcttcc
     2401 gggaaatgag ggagttcctg gtattcagtc ttctccgtag catcccagtg ccaccatcca
     2461 taggccccat attcctggtt gtaatacgcc ttcttaagtt cctttcgcgc aagataggca
     2521 ggtgtcgaca tcccgaaaac ttccatcggg aacggcttat cacttcgtgt atccagtaaa
     2581 taaaaatctg ggaaaacatc gctcgcactg gcgtcatacc gcataggctt aacatagtgc
     2641 cggtgttcgg catcaagttt acgggacact atcgcttcgt aggaggagtc cagaggtatc
     2701 cagttatcac tcaccagcat aagcactatc tgatgagctc gtgcagagat acctctactg
     2761 gtcaccgata cgggagatgt aagagcaaaa acaacaactt tgcccccatt tttccaggcg
     2821 gcatattcca gagggaatcg ccgcttcacg ctatcccagt gcccgtctga gttaaagaag
     2881 gtaaggggca taccgccgaa gttacgcata ggtagaaact tcagcggctt ctcatgcttt
     2941 tctgcgtcat agcgcggcag tactgatagc agcagcagtc gtttgtcgct cagttccgcc
     3001 gacgacagca gttgaacctg ctcactggcc actttgcttt tagagtccgc caccccgata
     3061 aaaagatggt cgccaataca tgctctaccc gatctaatct gctttgcggc ttccaacatc
     3121 cggtatcgaa ccagcgaatc atttcgcttc ccggccatat tcggatacca gacattaaga
     3181 ccggcttcag tccacaacaa actcagaagt ccggagaggg tcattgaagc ctggccacct
     3241 tcaggtcgct ggatttgggg cagggaagga acctcagact tttccggcgg atctttctcc
     3301 gtcatgccta ttccaaggcg gattgacatg gtcccctcat tgttgatgcg tacaacattg
     3361 ctggcataac cctttaaccc tgtatgcctg gcatcaaggt cgaaaaacac acacgatgga
     3421 tcatgctcag tgcctgtatt tgcagcctta atcaggatga acgtatcact gtttggtagt
     3481 gagcggacgt agagacgctt tgcgcctttt ccccgacatc cgcagctgat cacagatttc
     3541 tgcccggacg tgtggtgtgc tttcttcagg gccgactgcc atccctttac atactcatcc
     3601 tgtgtctgaa attcagccga gtatcgtttc gtgtttggtt cgtcatcgta agcaataaaa
     3661 actgcaaact tctggcccat gggacctcct gaaatttaaa aatactaata gcctagccat
     3721 atcaatactt cttgcgcgtt gtactcttcc ctctcaccca actcggaaaa aaatacgtct
     3781 atgtcgctgg ctacgacgcc atgttttgca agctcttgca gagctctctg ttttgaaatg
     3841 gtgaggtctt ctgctgaatc aaaatacgtc atcattacct cctgatgaag gagttgactg
     3901 aagcaggcct ttctctccct gcccaggttt ttttctgtgt aaaagttaca atattagtga
     3961 aataaaccat tacgttaacc tgcaaaatca cacagaagaa ttactatgat gccgcttcaa
     4021 actctccctt gagacgagcc gcaacggcga tctcaatacg gcttcgaagc aaactggcca
     4081 cggccgcaaa tacgtcgacg atgaccgaat tgccgaactg gcgataggcc tgggtatcag
     4141 agactggaat gcgaaaagta acttttcccg gttgctcgaa tcccataagg cgggcacatt
     4201 ctcgcggggt taaacggcgc gggcgccttg ccatatttac aggatcatta aagtcctttt
     4261 cgcctatgct gtgatcccat ccacggtcta gcaaaatttc agagccatca cgatagtacc
     4321 gtgcgctgag tgttctgcaa acaactgatt tatcaagtgg atttaccagt ccgaagccaa
     4381 atccattacc ttttgcctgg tgctttttag cgtagttata aaggtactcc cagagctttg
     4441 gagaaaggat gtaacggcta tcgacctcag cgtccagcag ctccccaagg gttggccgtc
     4501 gtgaaggata aagagccgat acaccactta acgaaaagcc atcatggagc ttcagatcgc
     4561 gcctgaatcc aaccaacacg atacgttccc ggtgctgagg cctgaagttc ttaccgtcaa
     4621 ttattttggg gtcctttgat ccgtgagcat cgcagtcggc aacatcgtag ccaagttcat
     4681 caagcgcttt catgatgatg cggaacgtat ttcccttatc gtggctcttc agatttttta
     4741 cgttctcaag aacgaaaatg gcaggctgtt tggctcggat gatccgggcg acatcaaaaa
     4801 aaagggtccc ctgggtgtcg cattcaaagc cgtgttttcg gcccaaagag ttctttttgc
     4861 tgacaccggc aatactgaac ggttggcatg ggaaacctgc gagcaaaaca tcatggtctg
     4921 ggatggatgc atcgatagaa gcataggcct ggtcatccga aacatcggga agattactga
     4981 gtgtaatgtc ccggatatct gagttgaaat agtgctcagt ctcgtcacag taccagtttg
     5041 cactgtatgt ccggcgggag aactgattcc attcgcttgt aaacacgcat ttcccgccaa
     5101 tggcgtcaaa accactccgg aggccaccga tcccagcaaa caagtcaata aaacggaaaa
     5161 atttctggtc atagtctgct gggcgtgagg ggagcaacga tcgaagataa tgatattctt
     5221 cctgggttat tcgacattca ttaactgaac ctttgatctg gcgaataagt gatgccctcg
     5281 accagtcgct gccggttgca gcattaagtt gcgcagccaa atcttttacg tcatagatac
     5341 ttgcaacctg caccagtaat ttatggatag aggaaggcgt ctcatcatta agcaatgctg
     5401 ttattgcagg ctcagttcta tcgttgtaca tccttactcc atgaaatgtg attaatttag
     5461 gacctcagac taccacgcgg tgaaaattca attttttgtt aaaggccttt attttcatct
     5521 cctgaattaa aatttttaat ccactcaaat tgcctccgtt aacggaaaat cgccgagttt
     5581 ctaattggta caataagcgc gtcaaatcac acgacttatt tgttactacg aaccatctaa
     5641 ttcagggaga acgtatgact ctgttgaaaa agttactttt gccgacatta atcgtccttt
     5701 tcccggcggc aactctggct gcccaatcgt catttgaagc gaaagtggta aaaattatcg
     5761 acggggacac aatcaccgcg ctcgatggtc agaatacaag catcaaaatc cgcttgtacg
     5821 gtattgatgc ccccgaaagt aagcaggcct ttggacaaaa gtctaaacaa gcgttgagcg
     5881 ccgcgattgc tgcacagaat attacggtaa tagaccacgg acctgatatt tacggcagga
     5941 tgctgggaac catttggctt gatggttatg acataaacgc ctcaatggta gacagcggct
     6001 atgcatgggt ataccggttt gacggtaatg ccatcgtccc aaactatctg aaattcgaag
     6061 cttcagctca gaaagcagtt aaaggcctct gggtagatcc taatcccgtt gccccctggg
     6121 agtggcgcca gcaaaaccag aaacctcaaa agacgaaaag cagggggtga ccgcgatgac
     6181 aagacagcat cctttattga aaattgcgct taatagcgat ggacatcttt cgtggccaat
     6241 tttaccagga gagactcacc gctgcccaac gtgtctgcga cacgttacta cgggcccgga
     6301 tggggatttg gtgcattcga tggaggaggg cggcaacgtc tgcatacctt cagcttctgt
     6361 tgttatttca aaggcaatta ttgaaatgct cagcgcaggc gaacgtctct atgttaatcc
     6421 aatcaagaat gggaataaaa ccctagcacc gtcatttatc tttctgcata cagaccagca
     6481 actccgccca tttatcaatt cggattatca gccagccggt gccagctgga gaagtgataa
     6541 aggtttcagg cttggccttt tctttatgaa tgagcgagcc tctaaaatta ataaagacca
     6601 gtttgatttt attgcagtaa tagacccatt gacctttcag tcagaatttg atgcgacatg
     6661 gtcccgtggt gaatttgatg accctctaaa ggcattacgg tattcaattg tttctgaaaa
     6721 cttgtcttcc gtctgggtga aatggcccgt aaaaaacctg caaccgagga aagaaacggt
     6781 gatggccgat tactggtatg actattattc acctcattcc ccagctcagc aggctgcttg
     6841 cacagcaacg gtgactggaa tagatgggaa tgtatccggc gaaactattt acatgctcca
     6901 gatcgcttta gagcggtcga cagttgagta ccagcttgta aaatcgctag gggtcatatt
     6961 gctactggat aatacaggaa atttggtatc ggaggcgcag ccgcactttg atataatcat
     7021 caaggcatgc atatcgggcg tcagagactt tgttcttcag aatcctcgga cgctgggttt
     7081 tgaaaacaag gttgcatagt tataaaaaat cttgccgttc tcaaggcatc attaaaaatt
     7141 aaaatgaggt atacatggta caagtactca acaccactgg tttgaattat caacttgaaa
     7201 aaacaatcac tgaagctgag gaaaggataa ttctcattag cccatacctt aagcttagta
     7261 atcgcattaa agagctgatc gaagataaaa atagactaaa agttgacatc agaatagttt
     7321 atgggaaatc agaactaaac tctaaagaat atgaatggtt aacaaacctg ccttttgtac
     7381 gccttagttt ttgtaaaaac ctccatgcaa aattatatgc caatgaaaat caatgtattg
     7441 tgtgcagtct aaacctttat gattttagtc agattaataa ccatgaaatt ggagtgttaa
     7501 ttattaagga gcaggagaag gatgcataca cttcatcatt aaatgaagct cagcgactga
     7561 tcagaatcag cacagataac attcagccac taacaacaga taaagaaaca acaggttcaa
     7621 aagaaagtga aaaaactaat aaagagtcat ccgacttcac ttcagccttt catgaagaac
     7681 cattaacaaa tcaaaatgga ttgattactg atttaaatac aaattcaaaa ctcacttcga
     7741 caaatctagc aaaaaatcat aaaactgatt tgttatctct atataaaaaa ttaatcgaag
     7801 ctggatatct catggacgta gaaggaaaat acatattgac tgatgctggc atagcggcag
     7861 gggcagaagc caagccaaat cgatataaaa aaggagaaaa ctattttctg tggcctgata
     7921 acctagcatt atgattttac atcacgtact gtgtccctgc gtagccatga gggacataga
     7981 agggtcaatg aaaaatagct tgtttagtac tgattcgaat actggggtca cctttaccat
     8041 ctcgcctcac ggccagggat gtaaactgtc tgtagagcct gaatatcgtc gcaaaggaac
     8101 acaaaattac gatggctggt tccctcgata ttacactaag ccacaatacg ctaaagcagc
     8161 attgacacgg tttcttgggg aacctgttaa ttggttggat tgtaatgacc ataattaggg
     8221 ctttcagaca gttagccaac aaaaaaatct ttaactcacc aacaatattg agaattacca
     8281 aatggaaaat ctggatgttg tatcagcggt agatttttac ttagaaaaag cattttattt
     8341 atttggattc ataatgttag gtatggcatg gcttactttt cgtgctggtt caacttatca
     8401 gcattttaaa aatgctgata agttagtcag tgaaggtaga ggctcagttt caagcggtaa
     8461 agagcctgaa atgatccggc ggcttaagtt ccaaacattg atctggtaca ttgctaattg
     8521 tgtggtgtta gcattatcca ctattgcttt tgggtattta ttttatcttc tacacagtta
     8581 atcaaacaat gagagatttt tattttctat aatcaggtac atatttattt tcttggagat
     8641 attgatgaac gaaaacaatc cacttctgca atctgaactg ctgttttgaa tacgttagtg
     8701 gaggtaatgc ctatggtatt agatatcatg actgttttgg cctttgcgtt tttggttctg
     8761 tataaaatag cttcacgaat catttctatg tctggtggtg cgataaggtt ctgattttca
     8821 gaaccttttt ttaaagttta ctagagtaga tttcattttg aataaacgta agatactcct
     8881 cactttgact gcctttccta aattttttct gtccttcaaa atctatatca ggtatgaaat
     8941 gtattaactg gctatcttgg taaagtgaaa gaacggaatt ttgattgagc accttagagt
     9001 ccattaattg ataaactgat tcaatacatg cacctataag aacatcacac aattgcacgc
     9061 ttgggctttc tttcgaatca atttgataga cttttttaag gtttagaggg aatgagatag
     9121 ttgctatctc ggaatgttta aactctcctt ccttatcaca attgatcaaa aagttaaggc
     9181 gttcattgta agttagcaga tttttagaac gatcatgctc aattgcatac tctccagagg
     9241 acatcatttc cgtgcgagaa attaatgcat ttaaaatagt aaatgaggca tcagtgctaa
     9301 ttccatcagt catgatggca tcaatgcaat ctgggtggtt aaatgccaaa ggaccaaaaa
     9361 actcttttaa cctttgccaa tcaagcctcc ttattttgct tattagcaaa ctcactgaat
     9421 ctaatgtttt ttctttcata gcattttgaa actgaagcag aacactgatg aatcccctaa
     9481 tgatttttat caaaatcatt aagttaaggt agatacacat cttgtcatat gatcaaatgg
     9541 tttcgccaaa aatcaataat cagacaacaa aatgtgcgaa ctcgatattt tacacgactc
     9601 tctttaccaa ttctgccccg aattacactt aaaacgactc aacagcttaa cgttggcttg
     9661 ccacgcctta cttgactgta aaactctcac tcttaccgaa cttggccgta acctgccaac
     9721 caaagcgaga acaaaacata acatcaaacg aatcgaccga ttgttaggta atcgtcacct
     9781 ccacaaagag cgactcgctg tataccgttg gcatgctagc tttatctgtt cgggcaatac
     9841 gatgcccatt gtacttgttg actggtctga tatccgtgag caaaaacggc ttatggtatt
     9901 gcgagcttca gtcgcactac acggtcgttc tgttactctt tatgagaaag cgttcccgct
     9961 ttcagagcaa tgttcaaaga aagctcatga ccaatttcta gccgaccttg cgagcattct
    10021 accgagtaac accacaccgc tcattgtcag tgatgctggc tttaaagtgc catggtataa
    10081 atccgttgag aagctgggtt ggtactggtt aagtcgagta agaggaaaag tacaatatgc
    10141 agacctagga gcggaaaact ggaaacctat cagcaactta catgatatgt catctagtca
    10201 ctcaaagact ttaggctata agaggctgac taaaagcaat ccaatctcat gccaaattct
    10261 attgtataaa tctcgctcta aaggccgaaa aaatcagcgc tcgacacgga ctcattgtca
    10321 ccacccgtca cctaaaatct actcagcgtc ggcaaaggag ccatggattc tagcaactaa
    10381 cttacctgtt gaaattcgaa cacccaaaca acttgttaat atctattcga agcgaatgca
    10441 gattgaagaa accttccgag acttgaaaag tcctgcctac ggactaggcc tacgccatag
    10501 ccgaacgagc agctcagagc gttttgatat catgctgcta atcgccctga tgcttcaact
    10561 aacatgttgg cttgcgggcg ttcatgctca gaaacaaggt tgggacaagc acttccaggc
    10621 taacacagtc agaaatcgaa acgtactctc aacagttcgc ttaggcatgg aagttttgcg
    10681 gcattctggc tacacaataa caagggaaga ctcactcgtg gctgcaaccc tgcttactca
    10741 aaatctattc acacatggtt acgttttggg gaaattatga ggggatctct cagtgctcag
    10801 catgtttgag tgctttactc gccgcgggtt gagaaatatg taaaacttca gccgcacgcg
    10861 ttaaggaacc acaagtcatt acagcataaa agatatcgag atgtcttaat ttcattccat
    10921 gtagcctact gattatttat ttctacggac tattctaacg aataaagtat aaataatcag
    10981 atatactcgt cataattcaa attttggttt agttgtcacg agaattaatt cgtaaacgtg
    11041 caagctaaat agattgacta ggggaatata cggggtaaga aagcggctcg gcatactgcc
    11101 ctataataaa ttgttaggag aagtacgccg agtagtgtgt agctattagg taaagtatgt
    11161 accaatttca ataaatattt taattaagat actgtttgaa atatcaacaa taaaggcacc
    11221 gaccatagga acgacaagga aagctttatg tgatggtcca aatgcttttg tgactgtttg
    11281 catattcgca attgctgttg gtgttgctcc cataccaaag ccacagtgac ccgcgctgat
    11341 cacgacagca tcataatctt tgcccatcat tttgaaggtg acaaagcagg caaatagcac
    11401 catgacaaca gtttgtacag caatgataat taatactggc cctgccatgc ttgccaattg
    11461 accaaatttt aatgacatta acgccattgc caagaaaagc gataaagcaa cgctacctaa
    11521 tacatcgacg gtcggctcaa acacttcgtg tttaaataca tgagtcagtg tattacggat
    11581 aataataccg acaaataaac accagacaaa agtaggcagt tgcagaaaag tatctttaaa
    11641 caatgcactg atatagccac caacaacaat acagataatc agcattgaaa tggtttcaat
    11701 aacgttattt gcattgattt ttcttttgac gcttggttgc tcaaaagctt caacgatagt
    11761 gtcgcgctct tgctcggttg ttttaggaat agagaccttt ttcaaaagat gacgggcaac
    11821 agggccgcca actaaaccac ccaacactaa tccaagtgtt gcacaagcca tcgctaattc
    11881 aacggcgcct gttacaccat atttatcagc gagaataggg ccccatgctc cggcattacc
    11941 ataaccacct gttagagtaa ttgaacctgc aattaagcca ataaatggac tttcattcat
    12001 catgacagcc atactcatgc cgacagtatt ttgaatggcg attaggatcg ttactgcaat
    12061 agttaataga actaacggct ttcctccttt aatcagtcga gaaaagtcag aacttagccc
    12121 gatagaggaa aagaatgtga gcattaataa actttgcaat gaagcatcaa aagtaaacga
    12181 ataacctgat gttttatcaa taattaacag aacaattgca acaataaagc caccaacgac
    12241 ggcttctggt atgtggtttt tttgtaggaa cggggtaaat tttacgacaa acattcctat
    12301 gagtagcgcg atacatgcga ctaataatgt ataactggca tctaggatca tttttttacc
    12361 tctattagtt taccagaatt ttcactataa caatttaaca atagtgaaaa atgaagttac
    12421 tttggttaac taatctttaa caatgagttt tattagggta tccataactt ttagttaata
    12481 gggtcataat tttagtgtga tcttaatcat gaaatattaa aaagattaaa ataacaacaa
    12541 tggtatttaa tgatgggtaa acctaaatac catgcgggat ccgcactatt ttcggtgata
    12601 gcaaaaaata aaaaagtata aataataaac aaaattataa gagaaggaga acatgataag
    12661 cgcaggccga gcaatttaaa gaagtgtgat atacaacata aaacagcagc atgacctttc
    12721 tatatttcgt tgcttatcga agtgttaatt ttcgggtgga ttactctgtt tgttgattaa
    12781 taacggagaa aaatatgatt gctgtaatat ttgaggtgca aatacaaccc gaccaacaaa
    12841 ctcgctattt gactttagct gaggagttaa gaccactatt aagtcatgta gctggtttta
    12901 tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg ctatcttggt
    12961 gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg aaagctcaac
    13021 aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat attactcgcg
    13081 aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt agataatcaa
    13141 ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg attggaaggg
    13201 ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga acaaggaaag
    13261 gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg gacaccatta
    13321 attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg agtattggcg
    13381 gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact gatattaata
    13441 acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa gtgaacttgc
    13501 gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg cgataggggc
    13561 ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg ggcgagcttg
    13621 gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga gcagtcattt
    13681 atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc atcgttattt
    13741 tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga tctccttaaa
    13801 ccatggcgta catgccagag tttccacgcc agtgcattta cgaaaagcac gtacttgcta
    13861 tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc aacagcaatg
    13921 gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc atgaaatggg
    13981 aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag attggagtga
    14041 acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt atgaatctaa
    14101 agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa aaggttatgc
    14161 tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat gatcaattca
    14221 aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat agcttgtcgt
    14281 aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc gacttgatgc
    14341 tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag tgcatataat
    14401 gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc gagagtttca
    14461 tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg atgacttagt
    14521 aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct ttccccttct
    14581 aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa
    14641 gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag cttctgggcg
    14701 agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa tgcgctgtta
    14761 atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt gacactctat
    14821 cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa atgaatagtt
    14881 cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc cttatcatgc
    14941 cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct aaccactttg
    15001 gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg cttggaaaaa
    15061 tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc gcatcgctgg
    15121 attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt
    15181 cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat accacctcag
    15241 cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt ggtttaatag
    15301 cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc ttttttatcg
    15361 ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt gaaaccaaaa
    15421 atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat tcggtataca
    15481 tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg caattgatag
    15541 gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga tggaatagca
    15601 tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc caagcctttg
    15661 tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc ggatttattg
    15721 cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta gttttccctg
    15781 ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga gtgatgtcta
    15841 tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc cttaccaatg
    15901 caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca ctaccaattt
    15961 gggatggctg gatttggatt attggtttag cgttttactg tattattatc ctgctatcga
    16021 tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt gcttagttat
    16081 ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa cccaagaggg
    16141 cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt atttatcttt
    16201 caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat cggcaagctc
    16261 ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta agtttttaag
    16321 cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga caatggcgtt
    16381 taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac caatgatagg
    16441 catcacttct tggata
//
LOCUS       DAOFQD010000109        16286 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.109, whole genome shotgun sequence.
ACCESSION   DAOFQD010000109 DAOFQD010000000
VERSION     DAOFQD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.109"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..95
                     /locus_tag="QBF46_003578"
     ncRNA           <1..95
                     /ncRNA_class="other"
                     /locus_tag="QBF46_003578"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(279..956)
                     /gene="narI"
                     /locus_tag="QBF46_003579"
     CDS             complement(279..956)
                     /gene="narI"
                     /locus_tag="QBF46_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDQ6675714.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(956..1666)
                     /gene="narJ"
                     /locus_tag="QBF46_003580"
     CDS             complement(956..1666)
                     /gene="narJ"
                     /locus_tag="QBF46_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDQ6675715.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHS
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1663..3201)
                     /gene="narH"
                     /locus_tag="QBF46_003581"
     CDS             complement(1663..3201)
                     /gene="narH"
                     /locus_tag="QBF46_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDQ6675716.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3198..6941)
                     /locus_tag="QBF46_003582"
     CDS             complement(3198..6941)
                     /locus_tag="QBF46_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDQ6675717.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7457..8848)
                     /gene="narK"
                     /locus_tag="QBF46_003583"
     CDS             complement(7457..8848)
                     /gene="narK"
                     /locus_tag="QBF46_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDQ6675718.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFCGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9187..10983
                     /gene="narX"
                     /locus_tag="QBF46_003584"
     CDS             9187..10983
                     /gene="narX"
                     /locus_tag="QBF46_003584"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDQ6675719.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10976..11626
                     /gene="narL"
                     /locus_tag="QBF46_003585"
     CDS             10976..11626
                     /gene="narL"
                     /locus_tag="QBF46_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDQ6675720.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11627..13021)
                     /gene="ychO"
                     /locus_tag="QBF46_003586"
     CDS             complement(11627..13021)
                     /gene="ychO"
                     /locus_tag="QBF46_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDQ6675721.1"
                     /translation="MSRFVPRIISFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13207..13560
                     /gene="ychN"
                     /locus_tag="QBF46_003587"
     CDS             13207..13560
                     /gene="ychN"
                     /locus_tag="QBF46_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDQ6675722.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13604..14299)
                     /gene="chaC"
                     /locus_tag="QBF46_003588"
     CDS             complement(13604..14299)
                     /gene="chaC"
                     /locus_tag="QBF46_003588"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDQ6675723.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14457..14687)
                     /gene="chaB"
                     /locus_tag="QBF46_003589"
     CDS             complement(14457..14687)
                     /gene="chaB"
                     /locus_tag="QBF46_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDQ6675724.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14957..16057
                     /gene="chaA"
                     /locus_tag="QBF46_003590"
     CDS             14957..16057
                     /gene="chaA"
                     /locus_tag="QBF46_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDQ6675725.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         3940 a         4368 c         4072 g         3906 t
ORIGIN      
        1 aaggttcgac ttgagcgcca gcgagagagc gttgccgcag gcaacgaccc gaagggcgaa
       61 gcgcgcagcg ctgagtaatc cttcccccac caccatcgct tccttaaata atcaaaacct
      121 gttactccga gctaattttg cccgatggca ccacgtttac cgggcctaca atatcctccc
      181 ctgtaggtcg gataagcgaa gcgcatccga caactcaccc cgcaccgtgc ccataaaaaa
      241 accccgccga agcagggtct atgtgaacta aaattcgctt agtgacgagc gcgcaccagc
      301 tggtacttac gtgtcagata ctccaccggt acgctccaga tgtgtaccag acgcgagaac
      361 gggaacagca ggaataacgt catccccagc accaggtgca gacggaagat aaacgccaca
      421 ccatcgaggt gttgagaagc gccaccgtgg aaggtcacca ccgactgcgc ccagccaacc
      481 agtttcatca tctcgctacc gtccatatgc tgagcggaga acggaatggt caacaggccc
      541 agcgcgcact ggataacgag cagcgacagg atcaggatat ccgctccggt agtggttgca
      601 cgcacgcgtg ggctgaacag acgacgcttc agcaacagca cgccgccaat cagacacagc
      661 acgccgctgg caccaccagc aaacatcgcc attttctgtt tcacttcaat cggcagccag
      721 gcttcataca tccagtgcgg cgtcagcata ccgaagaagt gaccaacaaa aatccccaga
      781 ataccgatat ggaacaggtt cgacgccagg ttcatccctt tgcgatccag catctggctg
      841 gacgccgcgc gccaggtgta ctgcccgtag tcataacgca gccagctacc aatcaggaag
      901 accgccccgg ctatgtacgg gtagatatca aagaagaaca tattcaggaa ttgcattagt
      961 gctgtcctcc ggtggtgata ttcagatatt gcggcgcgac ggcaccggca aagcgacgct
     1021 gatgagcagt gattgctgaa tcaccgcagc ctttgtcagc aaagaattta acctgctctt
     1081 cttcccagac agcatccagc gcctgcggcg tatcatcgcg cgcttcgtcg gcaatttttt
     1141 ccgccacttt gtcgctgtcg atagcggtat tggccaattt cagcagcaga tcaaacatca
     1201 cggcataacg gctttcacgc tgttgcagac gcgcgctcag caatgccaga atcggcgcga
     1261 tatctttcaa accttccacg gcttcgcttt gcggcagctg cgacaggtac tccagataca
     1321 gcggcagatg gtccggtaat tcgcggctgt ttaactgcaa actgtgctgc tcgtactgcg
     1381 ccagcaggtc caccatcgcc tggccgcggt cacgggattc accgtgaaca tgttcaaata
     1441 gcaacagtga cgtggcgcgg ccacggtcga acagttcgct gtactgggcc tgggcatcga
     1501 gcggatccat cgccgttaaa tcgcgcagga aaatgcccag cgcatgggca tcttctttcg
     1561 acagattttt cgacgcagca atcgcctcaa acatctcttg ttgatgctgc cataaggcag
     1621 catccggata ttcaaggaga cgcgatacaa tcacgagttc gatcatggat gcggctccgt
     1681 tttgctggtc acatcgatgg catcgatacg acggctgttg aacagattga atttggtatc
     1741 tgaaccgtgg cagccatcac caaaggtaaa gccgcagcca tttttctccg ggaaggcttc
     1801 ccgtgccagt tcacgatgac tactcggcac cacaaagcga tcttcgtagt tagcaatcgc
     1861 cagataacgg tacatctcct gtgcctgggc ttcggtcaga ccgacctctt ccagcgcacg
     1921 ggtatcaact ttaccgtcaa cggtttcagc acgtttgtaa tgacgcatcg ccagcatacg
     1981 tttcagtgcg cgcagtaccg gtttggtatc accggcggtc agcagattcg ccagatactg
     2041 taccggaata cgcaagcttt cgacgtctgg cagaatgccg ttgctaccca gctcacccgc
     2101 gtctgctgca gactgaatcg gagacagagg cggcacgtac cagaccatcg gcagtgtgcg
     2161 atattctgga tgcagcggca gcgccagttt ccattccatt gccattttat aaaccggcga
     2221 ctgctgtgcg gcttcaataa cgctcagcgg aataccgtct ttaatcgcct gctcgatgac
     2281 tttcggatcg ttcggatcga ggaacacctc cagctgacgc tggtaaagat ctttctcgtt
     2341 ctcggtgctg gctgcacgtt caatcgcgtc ggcatcgtac agcagcacgc caagataacg
     2401 gatacgaccg acacaggttt ctgagcacac ggtcggctga cccgcttcaa tacgcggata
     2461 gcagaagatg cacttctcag acttaccgct cttccagttg aagtagattt ttttgtacgg
     2521 gcatccggtg atgcacatac gccagccacg gcatttatcc tggtcgatca ggacgatgcc
     2581 atcttcttca cgcttgtaaa tcgcaccgct cgggcaggtc gccacacatg ccgggttcag
     2641 gcagtgttcg cacaggcgcg gcaaatacat cataaaggtg ttttcgaact ggctatacat
     2701 cgccttctgg atgttgtcga agttcttgtc tttcgccagt ttgtcaaact caccacccag
     2761 gtcatcttcc cagttcggcc ctttttcgat tttcgccatc cgttccccgg taatcagcga
     2821 acgcggacgg gcaatcggct gcgatttgct gccttccggc gcggtatgca ggttctgata
     2881 gtcaaaatcg aacggctcgt aataatcgtc gatccccggc agatgcgggt tagcgaagat
     2941 tttacccagc aacatggcac ggttacccat gcgcggctgc agtttgccgt tgattttacg
     3001 gatccagccg cctttgtatt tttcctggtt ttcccagtca gtcgggaagc cctgacccgg
     3061 cttggtttcc acgttgttga accacgcgta ttccacgcct tcacggctgg tccagacgtt
     3121 tttacaggta actgaacagg tgtggcagcc gatgcactta tcgagattca gcaccatgcc
     3181 gacttgtgaa cgaattttca ttttacgctc tcctgtacct ggtcattgcc ttcgccatct
     3241 aaccagtcaa tgttcttcat cttacgcact acaacaaact catcgcggtt agaacctacg
     3301 gtgccgtagt agttaaagcc gtatgccaga tgggcatagc cgccgatcat atgcgtcggt
     3361 ttcggcgtga tacgggtgac cgagttatgg ataccaccac gctgttgggt aatttccgaa
     3421 ccaggcaggt taacgatacg ttcctgcgcg tggtacatca tggtcatccc tgccggaaca
     3481 cgctggctga caaccgcacg ggcagtcaga gcaccgttgc tgttgaatac ttcaatccag
     3541 tcgttatcgg cgatacccag atctttggca tcggcttcac tcaaccagac caccggacca
     3601 ccgcgaccta aagtcagcat cagcaggttg tcgctgtagg tggagtggat accccacttc
     3661 tggtgcggcg tcaggaagtt gagcgctttt tccgggttgc cgttggattt ctggcctatc
     3721 acttctttca ccgaacgggt gtcgatcggc ggacgataaa ccagcaggct ttcaccgaaa
     3781 tcacgcatcc actggtgatc ctgatacagt tgctgacgac cagagagcgt acgccatggg
     3841 atcagctcgt gaacgttggt gtaaccggcg ttgtaagaaa cgtgttcatc ttccagacca
     3901 gaccaggtcg ggctggagat aattttgcgc ggctgtgcct gaatatcgcg gaagcggatc
     3961 ttctcgtctt ctttattcag cgccagatgc gtatggtcac gaccggtaaa ttcgctcagg
     4021 gcagcccagg ctttcacggc tacctgaccg ttggtttccg gtgccagtgt caggatcatc
     4081 tctgccgcat caattgcggt gttcagcatc ggctggcctt tcgccggacc ttccgctttg
     4141 gtgtagttga gcttacgcag cagatccatc tcgctctggg tgttccaggc aatcccttta
     4201 ccgccattac cgattttctc catcagcggg ccgatagagg taaagcgttc gtaagtcgcc
     4261 ggataatcgc gctctacgac cataatgtgt ggcgcggttt tacctgggat caggtcgcac
     4321 tcgccttttt tccagtcttt cacatccagc ggctgcgcca gttcagcggc agagtcatgc
     4381 tggataggca gcgtgacgat gtcggtttct ttacccagat ggccgacgca cacttcggag
     4441 aatttcttcg cgatggcttt gtagatttcc cagtcgcttt tcgcttccca ggccggatcg
     4501 accgccgcag acagcgggtg aataaacgga tgcatatccg aagtattcat gtcgtctttt
     4561 tcgtaccagg tcgccgtcgg caaaatgatg tcggaataga gacaggtgct cgacagacgg
     4621 aagtccagcg taaccaccag atccagcttg ccttccagac cattgtcctg ccagtccact
     4681 tcttccggct tcacgccgcc ctgttgcccc agatctttac cctggatacc gtgctccgtc
     4741 cccagcaggt acttgagcat aaactcatga cctttaccgg aagaaccgag caggttagaa
     4801 cgccagatga acaggttgcg cgggtggttt ttaccgtttt ctggttgttc tgccgcaaaa
     4861 cggatggaac cctctttcag ggatttcacc gtatagtcca ccggattcat cccggctttt
     4921 tccgcttcgc cagcgatagt cagcgggtta gtgcctaact gcggtgcaga cggcagccag
     4981 cccatgcgtt ccgcacggac gttaaagtcg atcaagtgtc cggtatagcg ggatttgtcc
     5041 gccatcggtg acagcaactc ttccgccgtg acggtttcat aacgccactg gctggagtgg
     5101 ttatagaaat aagaagtgct gttcatgtga cgcgccggac gctgccagtc aagggcaaac
     5161 gccagcggct gccagccggt ttgcggacgc agtttttcct gacctacata gtgcgcccag
     5221 ccgcccccgc tctgaccgac acagccgcag aaaatcagca tgttgatcag accacgatag
     5281 ttcatatcga ggtgatacca gtggttcagc cccgcaccga cgataatcat cgaacgaccg
     5341 tgcgttttat cagcgttatc ggcaaattca cgggcgatgc gaataatctg gctgcgagaa
     5401 acgccggtaa tctgctcggc ccaggccggg gtataagctt tcacatcgtc atagctggtt
     5461 gcacagttaa cgtcgttcag gccacgttcc agaccgtagt ttgccagcgt cagatcataa
     5521 acggtggtca ccagggcggt gctgccatca gccagttgca ggcgtttcac cggcagtttg
     5581 tgcagcagca cgttttccag ttccactttg ttgaagtgtt ccgtgccgtc gccaccaaag
     5641 tacgggaagc ctacctcagc gatctcatcc tggctaccca gcaggctcag ttgcagctcg
     5701 gtttcttcgc cagttttgcc gtcgcgctgc tcaagattcc atttgccctt ctcgccccag
     5761 cggaagccaa tagaaccgtt cggcgcaacc atttcgccat tggtattaaa ggcgacagtt
     5821 ttccattccg gattgttttc ctggcccagc gcatcaacca gatcagcagc gcgcaacata
     5881 cgacctgcag cgtagtaacc gtcgcgttct tccagcatca ccagcatcgg catgtcggtg
     5941 tagcgacgca catagtcggt gaaatactgg cttgggttgt cgaggtggaa ttcacgcagc
     6001 attacgtggc ccatcgccag cgccattgcc gcatcggtgc cctgtttcgg tgccagccac
     6061 agatcgcaca gtttggcgat ttcagcgtag tctggtgtga cggcaacagt tttggtgcct
     6121 ttgtaacgca cttcagtaaa gaagtgagca tccggggtac gcgtctgcgg cacgtttgaa
     6181 ccccaggcga tgatgtagct ggagttatac cagtcagcag attccggtac gtcagtttgc
     6241 tcgccccagg tttgcggaga cgcaggaggc aggtcgcagt accagtcgta gaagcttaag
     6301 caagtaccgc caatcagcga gagatagcgt gcacccgatg cgtaagaaac catcgacatt
     6361 gccggaattg gcgagaaacc agcaacacgg tccgggccgt agtttttgat ggtgtaaacg
     6421 ttagatgcgg cgatcagttc gttcacctcc tgccaggaag aacgaacaaa tccaccgcgt
     6481 ccacgcgcct gcttaaagct tttcgcttta tcggcgtctt caatgataga agcccatgcc
     6541 tcaaccggat cgctatgcag agccttcgct tcacgccaca ttttcatcag gcgtttgcgc
     6601 atcatcgggt atttcaggcg gttggcactg taaagatacc aggagtagct ggcaccgcgc
     6661 gggcagccgc gaggttcatg gtttggcaga tccggacggg tacgcggata gtcagtctgc
     6721 tgggtttccc aggtgaccag accgtttttg acgtagattt tccagctgca ggagccggtg
     6781 cagtttaccc cgtgggtaga gcggacgatt ttgtcatgct gccaacgctg gcgatatcca
     6841 tcctcccagt cacggttggt attgagaagc tggccatgcc catcggcaaa ggtttcaccc
     6901 ttctgtttga agtagcgaaa ccggtccagg aatttactca tcggttttct cctgtgggag
     6961 cctgtcggcc ctctgataaa tcgacattgc taatgtttaa gggcaagata acgctctgaa
     7021 acagcgtggg aattgataac gatcaagagt gatggggatg aaaaataaag taaattccta
     7081 agatacccct ttaggataat taacagccac cacataacat actgtaaatt aagacgtttt
     7141 atacctgtag ttatttaaac tattaagatt tttatgcaga tgggtattaa ggagtattcc
     7201 ccatgggtaa catattaatg gctcttaatg aaatccggcg aaataaacca tataacatta
     7261 gtatgttgta actaaataac gcataaaaaa agcgcgacat catgccgcgc aaaggataat
     7321 caaatatttt actctatata attcggttta caggaaggcg gcaagtgaat gaatcccagg
     7381 agcttactaa agtaagtgac tggggtgaat gaacgcagcc gcagcacatg taacccgaag
     7441 tatgacgagt aacggtttat tttttagaat gccgaccata taccgcccag gtaatcacta
     7501 cgcaggcgat atagaaaatc aaaaatacct tcattgcgcc gactggcgaa cccgttaatg
     7561 ccagcgagct accaaacgct ttcgggataa agaagccacc aatcgcgcca atcgcagaga
     7621 tgaaacccag cgccgccgcc gtgtcggttg ccgcttcacg catcgcacgt tcgtcagaac
     7681 ccccttctgc tttcacgcga tccattgtca gtttacggaa gatcactgaa atcatctgga
     7741 aagtggaacc actacccagc ccagctgtca ggaacagcgc caggaagacc gcgaagaacg
     7801 ccatgaagct tccgccctgc ccgtcagtcg gtaaggtcag gaacagcagg ccgctgaaaa
     7861 tcgccatcag aataaagttc accagcgtga cacgagttcc gcccagacgg tcagataatg
     7921 caccacctgc agaacgcgcc agcgcaccaa taaacggccc gaagaaagcg tattgcagaa
     7981 tctgaacatc cgggaactgc gtttttgaca gcattgcaaa gcccgcggag aagccgatga
     8041 aggagccgaa ggttgccaga tacagcaggc tcataatcca cagatgaccc cgtttgagta
     8101 ccggcaactg ctccttgatg gaggctttcg aggtagcaag atcgttcatg ccaaaccacg
     8161 ccgcaatggt gaagatggca aggaacggca cccatatcca ggacgcattc gccagataca
     8221 gctcagtccc atccggctgt ttgacgccct ggctaccaaa tactgcgaaa atcgacagtg
     8281 ataccaccag cggagcaacc aactgcatga cgctgacgcc catgtttccc agaccaccat
     8341 tcagacccag cgcgccaccc tgcttctgtt tcggaaagaa gaagctgatg tttgccatac
     8401 tggatgcgaa gttcgcgcca gcaaagccgc atagcagaga gatgatgatg aagacgctat
     8461 aaggcgtgga ggtatcctgc acggcaaaac ccagccagac gcaaggaata atcagaatac
     8521 cggtgctgaa cgccgtccag cgacgaccac agaagatagg aaccataaag gagtatggaa
     8581 cacgtaataa cgcgccagaa accgaaggca gcgcagtcaa cataaatagc tgatcggtcg
     8641 taaaattaaa accgactttc ggtaagttca ccgcaacagc gctgaacaac atccatacgc
     8701 aaaacgccag cagcagacag ggaacggaaa tccacaggtt gcggctggca atacgttgac
     8761 cgcgttgttg ccagaacgca ggatcttccg gtcgccaatc tgtaatgaca gctccagtag
     8821 ccctttcggg ggcggatgag tgactcatag acacctctga tactcgtttc gatgtctgcc
     8881 accttagtgt ctggagctaa aggcaatttg atgtaaatca aacgataaga taactttatc
     8941 tatcattgat atttatcatt acccatagtg agtacagtga tttcataaaa attatgagat
     9001 ttttcacggt gctgtaaaat ccctaccctt accgatgtaa agcgactaac cacacggcaa
     9061 ataaggagta actctttccg ggtatgggta tacttcagcc aatagccgag aatactgcca
     9121 ttccagaatg tatcgtcaca ttcattaagg ttattgctca tttaaagcct gaaggaagag
     9181 gtttacatgc ttaaacgttg tctctctccg ctcaccctgg ttaatcaggt tgcgcttatt
     9241 gtgttgcttt ctactgctat tggactggca gggatggcgg tttctggctg gctggtgcaa
     9301 ggcgttcagg gcagcgccca tgcgatcaac aaagcgggat cgctgcgcat gcaaagttac
     9361 cgtctgttgg cagcagtgcc attaagcgag aaagacaagc ccttaattaa agagatggaa
     9421 caaacggcat ttagcgccga gttgactcga gcagcagaac gagacggaca actggcgcaa
     9481 ttacagggtt tacaagatta ctggcgtaat gaactgatcc ctgcactgat gcgtgcacaa
     9541 aaccgcgaaa cggtgtcagc ggatgtcagc cagtttgttg ccgggcttga tcagctggta
     9601 tctggttttg accgcaccac ggaaatgcgc attgagacag tggtactggt ccatcgggta
     9661 atggcggtat ttatggcact tttactggtg ttcactatta tctggttgcg ggcgcgactg
     9721 ctacaaccgt ggcggcaact gctggcaatg gcgagtgccg tcagtcatcg cgattttacc
     9781 caacgcgcga acatcagcgg gcgcaacgaa atggcgatgc ttggaactgc attgaacaat
     9841 atgtctgcag aactggccga aagttatgcc gtacttgagc agcgggttca ggagaaaacc
     9901 gccgggctgg agcataaaaa tcagatcctc tcttttttat ggcaggctaa ccgccgtttg
     9961 cattcccgcg ccccgctgtg tgaacgcctg tcacctgtac tcaacggctt acagaattta
    10021 accctgctac gtgatatcga attgcgggtg tatgacactg atgatgaaga gaatcatcag
    10081 gagtttacct gccagccaga tatgacttgt gatgataaag gctgccagct ctgcccgcgc
    10141 ggcgtattac ccgttggcga tcgcggcaca accctgaagt ggcggctggc tgactcacat
    10201 acgcagtacg gtattttgct ggcgacccta ccgcaggggc gtcatcttag ccatgatcaa
    10261 caacaactgg tggataccct ggttgaacaa ctcaccgcca cgctggcgct ggatcggcat
    10321 caggaacgtc agcaacagtt gatcgtgatg gaagagcgtg ccaccattgc gcgcgaactg
    10381 catgattcta ttgcccaatc tctctcttgc atgaagatgc aggtgagttg tttacagatg
    10441 cagggcgatg cgctgccaga aagcagccgc gaactgttaa gtcagatccg taacgaactg
    10501 aatgcatcct gggcgcagtt gcgtgaattg ctcaccacat tccgcttgca gctcaccgag
    10561 cctggattac gtccggcgct ggaagcgagt tgcgaagagt acagcgccaa atttggcttc
    10621 ccggtgaagc tggattatca attgccgcct cgtctggtgc cttcacatca ggcaatccac
    10681 ttgttgcaaa ttgcccgtga ggcattaagt aacgccctca aacattcgca agcgagtgag
    10741 gtcgtggtga cggtggcgca aaacgataat caggtcaaac tgaccgtcca ggataacggc
    10801 tgcggcgtgc ctgaaaatgc catccgcagc aatcactacg gcatgataat aatgcgcgac
    10861 cgtgcgcaaa gtttacgagg cgattgccgc gtccgccgtc gtgaatcagg tggcaccgaa
    10921 gtggtggtca cctttattcc cgaaaaaact ttcacagacg tccaaggaga tacccatgag
    10981 taatcaggaa ccggctacta tcctgctgat tgacgatcac ccgatgctgc gaactggcgt
    11041 aaaacagctt atcagtatgg caccagatat caccgtggtt ggcgaagcga gtaatggcga
    11101 acagggtatt gaactggcgg agtctcttga tcccgatctg atcctgttag atctcaatat
    11161 gcccggcatg aacggtctgg aaacgctgga taaactgcgc gaaaagtccc tctcagggcg
    11221 cattgtggta ttcagcgtct ctaaccatga agaagatgtg gtcaccgcac tgaaacgcgg
    11281 cgcggatggc tatctgttaa aagatatgga accggaagat ctgctgaaag cattgcatca
    11341 ggcagctgct ggcgaaatgg tattaagcga agcattaacg cctgttctgg ccgccagctt
    11401 gcgcgctaac cgtgccacta ctgagcgcga tgttaaccag ttaaccccac gcgagcgcga
    11461 tattctcaag ctgattgccc agggtttgcc gaacaagatg attgcccgcc gcctggatat
    11521 caccgaaagc acagtaaaag tgcacgtcaa gcacatgctg aagaaaatga agctcaagtc
    11581 tcgcgtggaa gcagcggtat gggtgcatca ggagcgcatt ttctgattac ggttcccagc
    11641 gcagttcgtc gtttgacaat gcgtcgaacg gttcgacaag cgttagcgtg atctcattgg
    11701 aggagacacg ctgcccctgg ttatcttcca ccaccaccga caatcgccag tgattgctcg
    11761 ccccttcccc gttctgccag tcaggcatga tcagcgtcca gccctccgcg ctgttggctt
    11821 gtgcgcctgg cgtcaaactt aatatctgcg tatcgccctg ccaaatcagt tgccgaatac
    11881 cgtaacgact gcggatttgt aatttcagcg gcactgtttc gccaggtttt agatcccacg
    11941 gcggtgtcgc cagaaacacc gttaacgttt ttcgctgtcg gtactcaaga gtcggtagat
    12001 tatttcgctg cggattatca tagcgactac cacgtaacga ctgactttcg gcaacctcgc
    12061 ccgcagaaag ttgttttttg agcggtacac caaagcggta attaagattc agcccgaggt
    12121 tattttgatt ctcgccactt tcaccctgtt tatgctgggc cgtcacagtg actaatggca
    12181 caggggtgta atttaatccc agactcaacg cgacgggatt gtgataaccc gtaccagagt
    12241 taaacaaatc aacacgatca ccaaaatact gttctacgct gacactggta ttgaggtgtt
    12301 gatagaacgg catgcgcatc cgggctgtca ggtcgtaccc gcgcgccatc cgttgttcct
    12361 gcgtggctgt ctgttcatgc catgcagcaa atggctgata aaagtttgcc gatagtcgca
    12421 aatattcgcc ccacgcttcg gcaccaaagc ccgctcgctg aagattttcg tccagcaagt
    12481 tgtcataaaa agtgttataa cccaccagcc agttgccgcg cgcccagcgt tgcccaacgc
    12541 ccacattgct caccaaccca tcatcctgct gagtaagacc aagctggctc caggtgagat
    12601 aacgatcatt atcttgtaac ggcacaaacc agcttccacg actgccggta aaatgacctt
    12661 cgttatccac tttgacatca acactggcat ttccccacgg tgatagccag gactctacgt
    12721 gctgattaac ctgttgacta agcgcgtcgc ggacttttcc caatgcgaaa gcttttgcct
    12781 gctcgccagt atccagcccg ttatcattca tactggtttc gccgaaatct ttcacaattt
    12841 cagcaaagtg tttttcccca tcatgatttt caggtgccat gcctaaatcc ggcagaccat
    12901 cattgttatt atcaaaggga tttgccgctt tttgctcgaa ggtagattgt gcgttagctg
    12961 taccgcctgc cgccagcaag agtaaataaa acgaaataat gcgaggaacg aaacggctca
    13021 aaacgtcggt aacttccggt aaaaaatgaa aaaccatagc ctttaagaat aacgattact
    13081 ggcaactttt gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaatg tgaccgattg
    13141 gtcagacgct gcagaagcag taagatagaa gatgagtgtc taattccctg aatacaggag
    13201 taaataatgc aaaaaatcgt gatcgttgcc aatggcgcac cttacgggag cgaatccttg
    13261 tttaacagct tgcggctggc cattgcgtta cgagagcagg agagcaatct ggatctgcgt
    13321 ctgttcctca tgtctgatgc ggtcacagcc gggttgcgcg ggcaaaaacc aggggaaggc
    13381 tacaacattc agcaaatgct ggagatcctt accgctcaga atgtaccggt gaaattgtgc
    13441 aaaacctgta ccgacgggcg cgggattagt acacttcctc tgattgatgg ggtggaaatc
    13501 ggtactcttg tggaactggc gcaatggacg ctgtcagccg ataaagtgct cacattttaa
    13561 tgtttgaggg ggcgtctgac gccccacgat gagggaagtt tactcaggcg aatcccggcc
    13621 gtaacacacc atccggataa ttctccgcca gcagtttttt taccgatacc agcaaatcat
    13681 tcagcccatc gtcctgcatt cccagtttga taagctcctg ttccagtgaa aacaggtatt
    13741 gtgcattggt tcccagcgga ccgctcgccg ccgcaatcaa cggcgctatg acctgagcgc
    13801 gagtgtcaga ttcatattct ggatgtcgcg ggtccataat aaacacaatg gcgtttactg
    13861 tgcgtccatc atcaagatca agctgacacc aggttggcag ataacagccg gtaatcatct
    13921 cgcgcttcca caacagggtt agttcctgct ccagcgtctc ttctggcagt cgataggcga
    13981 cgcctgtggt gcgtccgccc tctttcagtg caagcatccg tcccggctgg tgcgcagtcc
    14041 cgcgcccagc ggtcaggcgc aggcaaaacg cgcgatgcca tccaaccagt gtaccggtgc
    14101 acgactcggt aaactccagt gccggattcc acatcaacga tccataaccg aagatccaca
    14161 ccggtccctc atcaggtcga caagccagcg tcgccgccag cgaagccgcc cgttgttctg
    14221 ctgaccataa gagtgattct tcaatggcac caaatgccgt tttacaatcg gcattcatca
    14281 agaaatcacg cgttatcacc ttacacctcc gccactgcat gcttccctgc gacaactttt
    14341 tggtgtcttc cggacatttt tactgctcat tttgaaacca ctaacgggac aatatgcaat
    14401 ccgcggacca cttgcaaggc aacaccgcac gaatagcttt aactaagcta ccggttttac
    14461 gattttttat gccatttatc atcatcccct ttggcatatt catgcttcac agcagcccag
    14521 gccactttat gcgcggtttc ttcgcgactg gcgtcatcac gccgatcttc tttatcttta
    14581 tattgatccc atgcgctgtt gaacgcttct ttatagatat cctgggcatg agacggtaga
    14641 acgtgcttta cgctttccgg cagatcgctt ttcgttttat acggcatcgt tgactccttt
    14701 tacaaagttg aacattaagt gtggtcaaca atatctactc ttgccaaaac ataagaattt
    14761 tcatgtcaga ctgttcttaa gaaacgctaa aacaaacatt gtctaaacgt aatacttatt
    14821 tttatgcccc aatgagtaaa aaatcgacag cggggtaaaa attcgtaaaa tgttaccgat
    14881 atccagacac tttctgttat ttttcctacc tattttttac attttcgata aattcatctg
    14941 caaagggata accataatgt caaatgctca agaggcggta aaaacccgcc acaaggagac
    15001 ttcgcttatt ttcccggttc tggcgctggt agtgctgttc ctgtggggaa gcagccagac
    15061 actaccagtg gtcattgcca tcaatcttct tgcgcttatt ggtattttaa gtagcgcctt
    15121 tagtgttgtc cgtcatgcgg acgtattagc ccatcgcctg ggagaacctt acggttcgct
    15181 tattcttagc ctttcagtgg ttattcttga agtcagtttg atttcagctt taatggcaac
    15241 cggcgacgcc gcgccaacgc taatgcgtga tacgctctat tcaatcatta tgattgttac
    15301 cggtgggctg gttggctttt cattattgtt gggcggtcgt aagtttgcca cccaatatat
    15361 gaatctgttt ggtatcaagc agtatttaat tgcgctgttc cccctggcga taatcgtact
    15421 ggtatttcca atggctctgc ctgcggcgaa tttttcaacc ggtcaggcgt tactggtagc
    15481 attaatttct gcggcaatgt atggcgtatt tttgctgatc cagaccaaaa cgcatcaaag
    15541 tttgtttgtc tacgagcacg aagatgacag tgatgacgac gacccgcatc acggtaaacc
    15601 gtctgcccat agcagcgtgt ggcatgctat ctggttgatt atccatctga ttgccgttat
    15661 tgcagtcacc aaaatgaatg ccagcccgct ggagacattg ctcgacagca tgaatgcccc
    15721 tgtcgccttt actggcttcc tggtggctct gttgattctg tcgccggaag gtttaggtgc
    15781 gttaaaagca gtgttgaaca accaggtcca gcgcgcgatg aatctgttct ttggttcagt
    15841 gttagcaacc atttcgctta ccgtacctgt cgtcacgcta attgccttta tgacgggtaa
    15901 cgaattacag tttgcacttg gtgcgccaga aatggtggtg atggtggcct ctttagtgct
    15961 gtgccatatc tccttctcca ccggacgtac taacgtgctc aatggcgcag cgcatctggc
    16021 actgtttgcc gcctatttga tgacgatatt tgcctgacag ttatatctcc tccgatgacg
    16081 gaaatcgggg gagataagat aaattcctgc ttattgtatt tcttcatact acctggacag
    16141 ctcgttaatc agaattgcga gcctgtacca caacctgcac gaaaccactg caacagctgt
    16201 tgaaaagccg gaatgtactc tttattgcaa acatttcagg gatgaagtga agatgaattg
    16261 agacgtagta tccacaccaa gccgga
//
LOCUS       DAOFQD010000110        15598 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.110, whole genome shotgun sequence.
ACCESSION   DAOFQD010000110 DAOFQD010000000
VERSION     DAOFQD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.110"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            73..315
                     /locus_tag="QBF46_003591"
     CDS             73..315
                     /locus_tag="QBF46_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675726.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            429..1067
                     /locus_tag="QBF46_003592"
     CDS             429..1067
                     /locus_tag="QBF46_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDQ6675727.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGASWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1064..1801
                     /locus_tag="QBF46_003593"
     CDS             1064..1801
                     /locus_tag="QBF46_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDQ6675728.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1801..3897
                     /locus_tag="QBF46_003594"
     CDS             1801..3897
                     /locus_tag="QBF46_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDQ6675729.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3944..4222)
                     /locus_tag="QBF46_003595"
     CDS             complement(3944..4222)
                     /locus_tag="QBF46_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675730.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMVQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4223..4366
                     /locus_tag="QBF46_003596"
     CDS             4223..4366
                     /locus_tag="QBF46_003596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675731.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4492..4902
                     /gene="psiE"
                     /locus_tag="QBF46_003597"
     CDS             4492..4902
                     /gene="psiE"
                     /locus_tag="QBF46_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDQ6675732.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4946..6421)
                     /gene="xylE"
                     /locus_tag="QBF46_003598"
     CDS             complement(4946..6421)
                     /gene="xylE"
                     /locus_tag="QBF46_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDQ6675733.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFSIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6793..7683)
                     /gene="malG"
                     /locus_tag="QBF46_003599"
     CDS             complement(6793..7683)
                     /gene="malG"
                     /locus_tag="QBF46_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDQ6675734.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7698..9242)
                     /gene="malF"
                     /locus_tag="QBF46_003600"
     CDS             complement(7698..9242)
                     /gene="malF"
                     /locus_tag="QBF46_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDQ6675735.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9396..10586)
                     /gene="malE"
                     /locus_tag="QBF46_003601"
     CDS             complement(9396..10586)
                     /gene="malE"
                     /locus_tag="QBF46_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDQ6675736.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10951..12066
                     /gene="malK"
                     /locus_tag="QBF46_003602"
     CDS             10951..12066
                     /gene="malK"
                     /locus_tag="QBF46_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDQ6675737.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12138..13478
                     /gene="lamB"
                     /locus_tag="QBF46_003603"
     CDS             12138..13478
                     /gene="lamB"
                     /locus_tag="QBF46_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDQ6675738.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNKKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13721..14641
                     /gene="malM"
                     /locus_tag="QBF46_003604"
     CDS             13721..14641
                     /gene="malM"
                     /locus_tag="QBF46_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDQ6675739.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14870..15598
                     /locus_tag="QBF46_003605"
                     /pseudo
     CDS             14870..15598
                     /locus_tag="QBF46_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579094.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3919 a         3932 c         4060 g         3687 t
ORIGIN      
        1 agttgtgtat actcgatccc gcccgaaatg tttttgggta aatctccatt cattcaatga
       61 agggaaattg ttatgaaaaa agttctgtat ggcatttttg ccatatctgc gcttgcggcg
      121 acttctgcgt gggctgcacc tgtacaggtg ggcgaagcgg cagggtcggc agcaacgtcg
      181 gtttcggcgg ggagttcctc cgcgaccagc gtcagcaccg taagctcggc ggtgggtgtc
      241 gcgctcgcgg caaccggtgg cggtgatggt tctaataccg ggaccaccac aaccacgacc
      301 accagtaccc agtaataaag tatgtatccc caaaataatt tgagtcattg catctgtggc
      361 ttgaagtatg aagggattta ccataaccac actccggtgt ggttattctg cccctctgga
      421 gaagagtcgt gaagcgacct gcactcattc ttatctgcct gctattacag gcctgttcag
      481 ccacgactaa agagctgggc aattcactgt gggacagtct gttcggcacg ccaggcgtac
      541 agctgacgga tgatgatatt caaaatatgc cctacgccag ccagtacatg cagcttaatg
      601 gcgggccgca gttatttgtg gtgctggcct tcgctgaaga cggacaacaa aaatgggtca
      661 ctcaggatca ggctactctc gttacccaac atggccgtct ggtgaagact ctgctcggcg
      721 gcgacaacct gattgaagtg aataaccttg ctgccgaccc gctgattaaa cccgcacaaa
      781 tcgttgatgg cgcaagctgg acgcgcacga tgggctggac cgagtaccag caggtacgct
      841 acgccaccgc acgctcagtc ttcaaatggg atggcaccga taccgtcaaa gtcggcagcg
      901 atgaaacccc ggttcgcgtg ctggacgaag aagtctccac cgaccaggcg cgctggcata
      961 accgctattg gatcgacagc gaagggcaaa ttcgccagtc ggaacagtat ctcggcgcgg
     1021 attattttcc ggtgaaaacc actctcatca aggcggcaaa acaatgatta aacaaactat
     1081 tgtcgcgttg cttttgagtg tgggagcgtc atcggtcttc gcggcaggaa ccgtcaaggt
     1141 gttcagcaat ggcagcagtg aggccaaaac gctaacgggc gcagagcatt taatcgatct
     1201 ggtaggccaa ccgcggctgg caaacagctg gtggcccggt gcggtgatta gcgaagagct
     1261 ggcaacggcg gcagcattgc gtcagcagca ggcgttgctg acacggctgg cagaacaggg
     1321 cgcagattcc agcgccgacg atgccgctgc gattaacgcc ttacgccagc aaattcaggc
     1381 gttgaaagtg acgggcaggc aaaaaatcaa tcttgatccc gatatcgtcc gcgttgccga
     1441 acgcggtaac ccgccgttgc agggcaacta cacgctgtgg gtcggaccac cgccgtccac
     1501 ggtcacgttg ttcgggctta tcagccgtcc tggcaagcag ccattcactc caggtcgcga
     1561 cgtggcgagc tatctctctg accagagcct gctcagcggt gcggatcgca gctacgcgtg
     1621 ggtggtttac ccggacggac gcacgcaaaa agcgccggtg gcttactgga acaagcgtca
     1681 cgtagagccg atgcccggca gcattattta tgttggcctc gcggactccg tctggagtga
     1741 gacgcctgat gcccttaacg ccgacattct tcagactctg acgcagcgga tacctcaata
     1801 atgaaaaaaa gacatctgct tagcttactg gcgctgggca ttagcacagc ttgctacggc
     1861 gaaacatatc ctgcgcccat tggtccgtcg cagtcggatt tcggtggcgt aggattatta
     1921 caaacgccca ccgcgcgtat ggcacgggaa ggggagttga gtctgaacta tcgcgataac
     1981 gatcagtacc gttattactc agcttcagtg caactcttcc cgtggctgga aacaacgctg
     2041 cgttacaccg acgtgcgcac ccggcagtac agcagcgtcg aagcgttctc tggcgatcaa
     2101 acgtataaag ataaagcctt cgatctcaaa ctgcgtttgt gggaagagag ttactggctg
     2161 ccgcaagtgg cggttggtgc gcgggatatc ggcggtacgg ggctgtttga tgcggaatat
     2221 cttgttgcca gcaaagcctg ggggccgttc gattttacgc tcggcctggg ctggggatat
     2281 ttgggcacca gcggtaatgt gaaaaatccg ctctgttcag ccagtgataa atattgctat
     2341 cgcgataaca gctacaaaca ggcgggatct atcgacggta gccagatgtt ccacggtcct
     2401 gcctcactgt ttggcggcgt ggaataccag acgccctggc aaccgctgcg cctgaaactg
     2461 gagtatgaag gcaataatta tcagcaggat tttgccggga agctggagca aaaaagtaag
     2521 tttaacgtcg gtgcgattta tcgcgttacc gattgggccg acgttaacct tagctatgaa
     2581 cgtggcaaca cctttatgtt tggcgttacg ttgcgcacca actttaacga tctgcgcccg
     2641 tcttacaacg ataacgcccg cccgcaatat caaccgcagc cgcaggatgc cattttgcag
     2701 cattcggtgg tggcgaatca gttaacgctg ttgaaataca atgctggact tgccgatcca
     2761 cagatccagg cgaaaggcga tacgctgtat gttaccggcg agcaggtgaa atatcgtgat
     2821 tcgcgcgaag ggatcatccg tgcgaatcgg atcgtgatga acgatctgcc ggatgggatc
     2881 aaaacgatcc gcattacgga aaatcgcctt aacatgccgc aggtgacgac ggaaaccgat
     2941 gtcgccagcc tgaaaaatca tctcgccgga gagccgttgg gccacgaaac gacgctggcg
     3001 caaaaacgcg tcgagccagt ggttccgcag tccaccgagc agggctggta tatcgacaaa
     3061 tcacgctttg atttccatat cgatccggtg ctgaaccagt cggtcggtgg cccggaaaac
     3121 ttttacatgt atcagctggg cgtgatggga acggcagatt tgtggctgac ggaccatctg
     3181 ctgaccaccg gcagcctgtt tgcaaatctt gccaacaact acgacaagtt taactacact
     3241 aatcctccgc aggactcgca cttaccgcgc gtgcgtaccc atgtgcgcga gtatgtgcag
     3301 aacgatgtct atgtgaataa cctgcaagcc aactacttcc agcatctggg caacggcttc
     3361 tacggtcagg tctacggtgg ttatctcgaa accatgtttg gcggtgcggg ggcagaagtg
     3421 ttgtatcgcc cgctggacag caactgggcg tttggtctgg atgccaacta cgttaaacag
     3481 cgcgactggc gtagtgcaaa agatatgatg aaattcaccg actacagcgt gaaaaccgga
     3541 catctgaccg cctactggac gccatctttc gctcaggatg tgttagttaa agccagcgtc
     3601 gggcagtatc tggcagggga taaaggcggc acgctggaga tcgccaaacg ctttgatagc
     3661 ggcgtggtgg tgggtggcta tgccacgatc actaatgttt caaaagagga gtacggcgaa
     3721 ggggacttca ccaaaggcgt gtatgtctct gtaccgttgg atctcttctc gtctggcccg
     3781 acacgcagcc gtgcggcgat tggctggacg ccgctgacgc gtgacggtgg tcaacaactt
     3841 gggcgtaagt tccagttgta tgacatgacc agcgaccgta gcgtcaattt ccgctaagtc
     3901 atgggaaagg tgccagtttt cgcaatcact ggcaccctca ttcttaatgg cctgttgagc
     3961 catcaggaac gcagttcacc cccggtttgc gatctggtgt gtaaaggcga aaacattctg
     4021 tcttcctgtc cgtcgatatc ggttgcaggt ctggttgcct gagcgactgg accatcattg
     4081 ggctggagac attgctttgc ggtaggtcgg ctttatcagc agtaccgagc ggaccagcat
     4141 aagcaggaag aacagagact gataacatca aagcagcaaa ataaggcttc atttttacca
     4201 cctttatcag gttacgtttc atttgttcca gaggaacatt gtcgattttt cgcgcattgc
     4261 tggtggctgg gaatcacctg aatgggtgat ttttgaatta ccggctttgg tgcggtttgt
     4321 cttgccggat gcgccgccag gcgcggctta tccggcctac gggtaggtat atccggcttt
     4381 ggaggaggcg cgctccaaat ccaggttgaa caaaacatac acaaaaaata tagatctccg
     4441 tcacattttt gcgttataca ggaagctcgc cactgtgaag gaggtactgc tatgacgtca
     4501 ctctctcgtc cgcgcgtgga gtttatctcc accattttgc agaccgtact caatcttggc
     4561 ctgctgtgcc tcggcctgat tttggttgtc ttcctcggca aagaaacggt gcatctggct
     4621 gatgtgctgt tcgcgccaga acaaaccagc aaatatgagc tggtagaagg actggtggtt
     4681 tactttctct atttcgaatt tatcgcgctg attgtgaagt actttcagtc cggttttcac
     4741 ttcccgctac gttactttgt ctatattggg atcaccgcca ttgtgcgctt gatcatcgtc
     4801 gatcataaat cgccactgga tgtgttgatc tactcggctg cgatcctgtt actggtgatc
     4861 accctgtggc tgtgtaattc gaaacggctg aagcgggagt aaaaagacag cacgccgaac
     4921 tggcgcggcg tgctggacag gaagattaca gcgtagcagt ttgttgtgtt ttcttcgttt
     4981 ccggttccca gagggcttcc agctcctcaa gggttttacc tttggtttcc gggacaaatt
     5041 tccacataaa cagtgctgcc agaacgccca tacacccgta aatccagtag gagaaaccgt
     5101 tgtggaaatg ggccaccagc caggagtttt tgtccatcat cgggaaggtc caggagacga
     5161 agtagttcgc cagccactgg gccgccaccg cgattgccag cgctttacca cgaatggcat
     5221 tcgggaagat ttccgacagc agaacccagc ataccggacc ccaggacatg gcaaaggcgg
     5281 caacatagaa cagcatcgac agtagcgcca caatacccgg tgcctgagtg taaaacgcgg
     5341 taccgaggct aaacataccg attgccattc cgagcgcgcc gataatttgc agtggcttac
     5401 gaccaaactt atctaccgtc ataattgcca gaacggtgaa ggtgaggttg ataactccga
     5461 caataatggt ctgcaacagc gcgatatccg tgctggcccc cagcgttttg aatacttccg
     5521 gcgcgtagta cagcaccaca ttgatgccga caaattgctg gaagatggag agcataacgc
     5581 cgatgacaat cacgcccacg ccaaacatca gcagacgacc accggttttg cggccatgat
     5641 ccagggagtg tttaatttcc tgtactgcct gagttgcaag ggtgttgccc ataattttgc
     5701 gcaggatacc ttccgcctgt tcttgcttgc cgcgtgacat cagccagcga ggactttctg
     5761 gcacggtata cagcagcatt aagaacagca gtgcagggat acattccgag gcaaacatat
     5821 aacgccagcc gtcagtattc agccaggagg cgtcgccgga acgggcaata aaatagttca
     5881 cacagtaaac taaaagttgc ccgaaaataa tcgaaaactg gttaaatgaa accagtttcc
     5941 cgcgaatatg agctggagcc agttccgcaa tatacattgg tgaaagcatt gaggctaaac
     6001 caacgccaat accgccaata atacgataaa taacaaattc cgggacataa cctgccagat
     6061 aaacaggcac tgtgttgtcc gggtttatag aggtaaaacc aagttctggc caggcagaac
     6121 ctacaccaga aataaaaaac aggacagcag caatcttaag tgaatcacga cggccgaagc
     6181 ggttactgca ataaccaccg agggcaccgc cgatgatgca accaatcaga gcgctggcta
     6241 cgcaaaatcc taacagggag ttggcagcgg attcacttaa gttttgtgga gcaacaaaga
     6301 cggtattgag tgactcaaca gtaccggaaa taacggcggt gtcgtagcca aataataaac
     6361 cacctaatgt agcgactaag gtaatcgaaa atatataact ggaattatac tgggtattca
     6421 ttcagacctg ccttagacca ttctgatctt ttatcagtgc attgatgttg gaattgtcag
     6481 gcagttaagg caattatcat tttttacaat gcgtttacgt aatgtctttt ctgtgatctt
     6541 aattgtgata attatcaaaa attgatgaaa acaatactat tgccgtgact cagagcacga
     6601 aagagaatta tcgtaagtgg gaaaacaaat aacgtaaaaa taataagctc tatgatggga
     6661 atattaaccg gcgaacgatt cagattgcag acgaaagaaa aaaaggcgct ccgtggagcg
     6721 ccgaataaca gtcacaagtt gggataacgt aagttgaggg tgcagcggca taacattggc
     6781 agaacaacat ctttaacctt tcacaccacc tgccgtcagg ccgttgacca gccagcgttg
     6841 agccagcaag aagacgatgg tgatcggtaa tgcagacatc acggcagcgg cggcaaagtc
     6901 accccacagg tagttttgcg ggttgaggta ttgctgcatc cccacggcca gggtgtagct
     6961 gtttacgtca cgcagtaaca gcgacgcgac cggaacttca gtaatggcag cgataaacga
     7021 caggatgaat accaccgcca gaatcggtac tgacagcggc aacaggacaa ggcggaaggc
     7081 ctgccacggt gtcgcaccat ccagcgcagc cgcttcttcc agcgaactat cgatggtttc
     7141 gaaatagcct ttgatggtcc agacatgcag cgcaatccca cccagatacg cgaaaattac
     7201 gccgccgtga gtattcaggc caatgaatgg aatgtactca cccagacgat caaacaacgc
     7261 atacaacgcg accagtgaaa gtactgccgg gaacatctgg aaaatcagca ttcctttcag
     7321 cagcgtcgct ttgcctggaa agcgcatacg ggcgaaagcg taggcgcagg tggtagagag
     7381 cgccacaatg ccaatcgcgg aaatcccggc gacctttacc gagttccata gccacagcag
     7441 taccgggaat ggcggtggcg taatgcgacc atcagcctgt tccacgctaa aacctaacgc
     7501 cagtttccag tgatcccagg agatttgctc cgggatcagg ctgccggtcg caaagttccc
     7561 ctggcgcagt gagatagcga cgaccatcag cagcgggaac ataatcgccg cgataaaaag
     7621 tagcagtagc aggtgagtaa taaacaaacg tgctttttgc gatttcggtt ggaccattgc
     7681 catttttgtt atctccctta atcaaacttc attcgcgtgg ctttcaggtt cactatcgcc
     7741 agtgcaccca ccagcaggaa gatcagcgtg gcaattgctg cagccagacc gaagtcctga
     7801 cccccgccgc cttcaaaagc gatacggtag gtgtagttaa caagcaggtc ggtataaccg
     7861 gctggcgtgg tcgtgccaag acgatccggg ccgccgttgg ttaacagctg aatcagcacg
     7921 aagttgttaa agttaaaggc gaagctggcg atcatcagcg gcgtcagcgg tttaatcagc
     7981 agcggcagcg taatcttaaa gaagttctgg aacggacctg cgccatccat tgctgaggct
     8041 tcatacaaat cgtccggaat cgctttcagc aagcccatgc agaggatcat catgtacgga
     8101 taacccagcc aggtattgac gataatcagc atcgtgcggg cggtggtcgg atcgctgaac
     8161 caggcgggct tcacgccaaa cagcgcgctc aacatcatgt tgatttcacc gaagctctgg
     8221 ttaaacaatc ctttgaaaat caagattgaa atgaacgatg gcaccgcgta gggcagaatc
     8281 agcaggacgc gatagaccgc tttgccgcgc aacgcttccc actgcaccag acacgccaga
     8341 accatgccga ccgccaccgt taaaaagaca gtgatcagcg agaacaccac ggtccagacg
     8401 aaaatggcga ggaacggttt ctgaatgcct tcgtcggtga agacgcgggt aaagtttttc
     8461 cagccagtag tcacggtgta accggggctt agcttttcat caccccagtt gccgtcggcg
     8521 gtaatggact ggtaaaagcc aatttggtta ttcggacgat atttcacgcc gctctgatta
     8581 ttcgtcaacg tgccgtcacc gtcgagtgtg tagagcggct gcgtgccaga aaactggcgc
     8641 agggagctca tcatcacttt gttgccatcc ggcagaatgg cggtaatgtc actcagcgcc
     8701 tgacgattct gggtaatcac gcgtagattc gcgcgttcgc cttcgggctg ggcggtcgtt
     8761 tctttcagtt gcagtttttg ttcgccgcca aatttaaacg cgtcggagag gtaatttttg
     8821 ccggtttcgc cgtcactgag cgccagttgc cactcatcgc ccgccgggta aagaccaaag
     8881 ttataggttt tgcctgcttg ccaggagcga tctaacaaca cttcctgcgc acgttcaaaa
     8941 gtcagctggt tagtgctgct gtagttggtg aaggcaatgg cgatggtgca gaccagaggg
     9001 aagaggacga ataatcccat tccagccatt cccgggtaaa catagcgcca ggcgtaggct
     9061 ttacgattgg cgaaaatata cagccccgct gaactcaata tcagcgtggt aatggcgaac
     9121 aggtattccc cttgtgcgta cattaaaaca acaaggtaac ccaccagcag gccgagcaga
     9181 cctagcactg accatttcag cgcgtcgctt tgccaccaat gtttcttttt aatgacatcc
     9241 atggggttct tcctcattcc aggacggata aggctttcac gccttatccg acaacaactg
     9301 cctgatgcga cactgatgcg tcttatcagg cctacatgcg tttcagtttt gtaggccgga
     9361 caaggcgttc acgccgcatc cggcatttca tagcattact tggtgatacg agtctgcgcg
     9421 tctttcaggg cttcatcgac agtctgacga ccgctggcgg cgttgatcac cgcagtacgc
     9481 acggcatacc agaaagcgga catctgcggg atgttcggca tgatttcacc tttctgggcg
     9541 ttttccatgg tggcggcaat acgtggatct ttcgccaact cttcctcgta agacttcagc
     9601 gccactgcac ccagcggttt gtctttattg accgcttcca gaccttcgtc agtcagcaga
     9661 tagttttcga ggaactcttt cgccagctct ttgttcggac tggcggcgtt aataccggcg
     9721 ctcagcacgc caacgaacgg tttagatggt tgacccttga aggtcggcag taccgttaca
     9781 ccataattca ctttgctggt gtcgatgttg gaccatgccc acgggccgtt gatggtcatc
     9841 gctgtttcgc ctttattaaa ggcagcttct gcgatggagt aatcggtgtc tgcattcatg
     9901 tgtttgtttt taataaggtc aaccaggaag gtcagacccg ctttcgcacc agcgttatcc
     9961 acgcccacgt ctttaatatc gtacttgccg ttttcatact tgaacgcata acccccgtca
    10021 gcagcaatca gcggccaggt gaagtacggt tcttgcaggt tgaacatcag cgcgctctta
    10081 cctttcgctt tcagttcttt atccagcgcc gggatctctt cccaggtttt tggtgggttc
    10141 ggcagcagat ctttgttata aatcagcgat aacgcttcaa cagcgatcgg gtaagcaatc
    10201 agcttgccgt tgtaacgtac ggcatcccag gtaaacggat acagcttgtc ctggaacgct
    10261 ttgtccgggg tgatttcagc caacaggcca gattgagcgt agccaccaaa gcggtcgtgt
    10321 gcccagaaga taatgtcagg gccatcgcca gttgccgcaa cctgagggaa tttctcttcc
    10381 agtttatccg gatgctcaac ggtgacttta attccggtat ctttctcgaa tttcttaccg
    10441 acttcagcga gaccgttata gcctttatcg ccgttaatcc agattaccag tttaccttct
    10501 tcgattttgg cgagagccga ggcggaaaac atcatcgtcg ttaatgcgga taatgcgagg
    10561 atgcgtgcac ctgtttttat tttcataatc tatggtcctt gttggtgaag tgctcgtgaa
    10621 aacacctaaa cggactctag tttctttata cggcaacctc tttccatcct ccttgcccct
    10681 acgccccacc gttgctttgt gtgatctctg ttacagaatt ggcggtaatg tggagatgcg
    10741 cacataaaat cgccacgatt tttgcaagca acatcacgaa attccttaca tgacctcggt
    10801 ttagttcaca gaagccgtgt tctcatcctc ccgcctcctc ccccataaaa aagccagggg
    10861 gtggaggatt taagccatct cctgatgacg catagtcagc ccatcatgaa tgttgctgtc
    10921 gatgacaggt tgttacaaag ggagaagggc atggcgagcg tacagctgca aaatgtaacg
    10981 aaagcctggg gcgaggtcgt ggtatcgaaa gatatcaatc tcgatatcca tgaaggtgaa
    11041 ttcgtggtgt ttgtcggacc gtctggctgc ggtaaatcga ctttactgcg catgattgcc
    11101 gggcttgaga cgatcaccag cggcgacctg ttcatcggtg agaaacggat gaatgacact
    11161 ccaccagcag aacgcggcgt tggtatggtg tttcagtctt acgcgctcta tccccacctg
    11221 tcagtagcag aaaacatgtc atttggcctg aaactggcag gcgcaaaaaa agaggtgatt
    11281 aaccaacgcg ttaaccaggt ggcggaagtg ctgcaactgg cgcatttgct ggatcgcaaa
    11341 ccgaaagcgc tctctggcgg tcagcgtcag cgtgtggcga ttggccgtac gctggtggcc
    11401 gagccaagcg tatttttgct cgatgaaccg ctctccaacc tcgatgctgc actgcgtgtg
    11461 caaatgcgta tcgaaatctc ccgtctgcat aaacgcctgg gccgcacaat gatttacgtc
    11521 acccacgatc aggtcgaagc gatgacgctg gccgacaaaa tcgtggtgct ggacgccggt
    11581 cgcgtggcgc aggttgggaa accgctggag ctgtaccact atccggcaga ccgttttgtc
    11641 gccggattta tcggttcgcc aaagatgaac ttcctgccgg taaaagtgac cgccaccgca
    11701 atcgatcaag tgcaggtgga gctgccgatg ccaaatcgtc agcaagtctg gctgccagtt
    11761 gaaagccgtg atgtccaggt tggagccaat atgtcgctgg gtattcgccc ggaacatcta
    11821 ctgccgagtg atatcgctga cgtcatcctt gagggtgaag ttcaggtcgt cgagcaactc
    11881 ggcaacgaaa cccaaatcca tatccagatc ccttccattc gtcaaaacct ggtgtaccgc
    11941 cagaacgacg tggtgttggt agaagaaggt gccacattcg ctatcggcct gccgccagag
    12001 cgttgccatc tgttccgtga ggatggcact gcatgtcgtc gactgcataa ggagccgggc
    12061 gtttaagcac cccacaaaac acacaaagcc tgtcacaggt gatgtgaaaa aagaaaagca
    12121 atgactcagg agatagaatg atgattactc tgcgcaaact tcctctggcg gttgccgtcg
    12181 cagcgggcgt aatgtctgct caggcaatgg ctgttgattt ccacggctat gcacgttccg
    12241 gtattggctg gacaggtagc ggcggtgaac aacagtgttt ccagactacc ggtgctcaaa
    12301 gtaaataccg tcttggcaac gaatgtgaaa cttatgctga attaaaattg ggtcaggaag
    12361 tgtggaaaga gggcgataag agcttctatt tcgacactaa cgtggcctat tccgtcgcgc
    12421 aacagaatga ctgggaagct accgatccgg ccttccgtga agcaaacgtg cagggtaaaa
    12481 acctgatcga atggctgcca ggttccacca tctgggcagg taagcgcttc taccaacgtc
    12541 atgacgttca tatgatcgac ttctactact gggatatttc tggtcctggt gccggtctgg
    12601 aaaacatcga tgttggcttc ggtaaactct ctctggcagc aacccgctcc tctgaagctg
    12661 gtggttcttc ctcttttgcc agcaacaata tttatgacta taccaacgaa accgcgaacg
    12721 acgttttcga tgtgcgttta gcgcagatgg aaatcaaccc gggcggcaca ttagaactgg
    12781 gtgtcgacta cggtcgtgcc aacctgcgtg ataactatcg tctggttgat ggcgcatcga
    12841 aagacggctg gttattcact gctgaacata ctcagagtgt cctgaagggc tttaacaagt
    12901 ttgttgttca gtacgctact gactcgatga cctcgcaggg taaaggtctg tcgcagggtt
    12961 ctggcgtcgc gtttgataac aaaaaatttg cctacaatat caacaacaac ggtcacatgc
    13021 tgcgtatcct cgaccacggt gcgatctcca tgggcgacaa ctgggacatg atgtacgtgg
    13081 gtatgtacca ggatatcaac tgggataacg acaacggcac caagtggtgg accgttggta
    13141 ttcgcccgat gtacaagtgg acgccaatca tgagcaccgt gatggaaatc ggctacgaca
    13201 acgtcgaatc ccagcgcacc ggcgacaaga acaatcagta caaaattacc ctcgcacaac
    13261 aatggcaggc tggcgacagc atctggtcac gcccggctat tcgtgtcttc gcaacctacg
    13321 ccaagtggga tgagaaatgg ggttatgact acaacggcga tagcaaggtt aacccgaact
    13381 acggcaaagc cgttcctgct gatttcaacg gcggcagctt cggtcgtggc gacagcgacg
    13441 agtggacctt cggtgcccag atggaaatct ggtggtaata gcaaaacctg ggccggataa
    13501 ggcgtttacg ccgcattcgg caaccaacgc ctgatacgac gcttgcgcgt cttatcaggc
    13561 ctacaacggc tgtcaaatgt aggccggata aggcgtttac gccgcatccg gcataaaaac
    13621 aggttgtcat tatctgaaag gggcgaaagc ccctctgatt atcgggttta gcgcgctatt
    13681 gcctggctac cgctgagctc cagattttga ggtgaaaaca atgaaaatga ataaaagtct
    13741 catcgccctc tgtttatcag cagggttact ggcaagcgcg cctggaatta gccttgccga
    13801 tgttaactac gtaccgcaaa acaccagcga cgcgccagcc attccatctg ctgcgctgca
    13861 acaactcacc tggacaccgg tcgatcaatc taaaacccag accacccaac tggcgaccgg
    13921 cggccaacaa ctgaacgttc ccggcatcag tggtccggtt gctgcgtaca gcgtcccggc
    13981 aaacattggc gaactgaccc tgacgctgac cagcgaagtg aacaaacaaa ccagcgtttt
    14041 tgcgccgaac gtgctgattc ttgatcagaa catgacccca tcagccttct tccccagcag
    14101 ttatttcacc taccaggaac caggcgtgat gagtgcagat cggctggaag gcgttatgcg
    14161 cctgacaccg gcgttggggc agcaaaaact ttatgttctg gtctttacca cggaaaaaga
    14221 tctccagcag acgacccaac tgctcgaccc ggctaaagcc tatgccaagg gcgtcggtaa
    14281 ctcgatcccg gatatccccg atccggttgc tcgtcatacc accgatggct tactgaaact
    14341 gaaagtgaaa acgaactcca gctccagcgt gttggtagga cctttatttg gttcttccgc
    14401 tccagctccg gttacggtag gtaacacggc ggcaccagct gtggctgcac ccgctccggc
    14461 accggtgaag aaaagcgagc cgatgctcaa cgacacggaa agttatttta ataccgcgat
    14521 caaaaacgct gtcgcgaaag gtgatgttga taaggcgtta aaactgcttg atgaagctga
    14581 acgcctggga tcgacatctg cccgttccac ctttatcagc agtgtaaaag gcaaggggta
    14641 attacgcccc acagtgctga ttttgcaaca actggtgcgt ctcctggcgc accttttttt
    14701 atgcttcctt cctgggatat gagcgatttt ttatagtaac tcacttcttc ttcactaaga
    14761 atatccatta tctcaatgcc ttatcagaga ttcttttcct ttcgccggta gtgtctggac
    14821 attcaggcta cttttccagg ttattttatt tctgttatgc agaggtttta tgataagtca
    14881 tatcctaaat tctggcggca ataactcttt gatgaaacat gatgtggtgc aaggaaataa
    14941 tatagtagat cttgatttac tacgtaattt aaatggggtg ccaggtttaa acagagataa
    15001 ctttatttat atcagcaata ttttttcaaa tataaaacaa cggaacgaaa aaatcatgca
    15061 ataaatatgt ttcgtgaagt ctcaatcagt aatgatacaa taagtgtaaa attctacaga
    15121 aatgaaaaaa attgaatgcg cttgcaattt tctgatggat aaagatgcgc aggggtatat
    15181 cgacctgtct gatttggatt taacaagttg tcattttaaa ggtgacgtta tatcgaaggt
    15241 gtctttttta tcatcaaatc tacaacatgt aacattcgaa tgtaaagaaa ttggggattg
    15301 caattttact actgcaatag ttgataatgt catatttaga tgtcgacgtt tacacaatgt
    15361 gatttttatc aaagcgagtg gtgaatgtgt cgatttcagc aaaaatattc ttgatacagt
    15421 tgacttctcg cagagtcaac ttggtcatag taattttcgc gaatgtcaga ttagaaattc
    15481 aaacttcgat aattgttatc tttacgcttc gcacttcacc agagcagagt ttctgtctgc
    15541 caaagaaata tcatttatta aatcgaattt gacagctgtt atgtttgatt atgtgcga
//
LOCUS       DAOFQD010000111        15434 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.111, whole genome shotgun sequence.
ACCESSION   DAOFQD010000111 DAOFQD010000000
VERSION     DAOFQD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.111"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..362)
                     /locus_tag="QBF46_003606"
     CDS             complement(<1..362)
                     /locus_tag="QBF46_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675740.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALY"
     gene            529..3378
                     /gene="rcsC"
                     /locus_tag="QBF46_003607"
     CDS             529..3378
                     /gene="rcsC"
                     /locus_tag="QBF46_003607"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDQ6675741.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLSVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3578..4228)
                     /gene="rcsB"
                     /locus_tag="QBF46_003608"
     CDS             complement(3578..4228)
                     /gene="rcsB"
                     /locus_tag="QBF46_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDQ6675742.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4245..6917)
                     /gene="rcsD"
                     /locus_tag="QBF46_003609"
     CDS             complement(4245..6917)
                     /gene="rcsD"
                     /locus_tag="QBF46_003609"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDQ6675743.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDNWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTENVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7656..8762
                     /gene="ompC"
                     /locus_tag="QBF46_003610"
     CDS             7656..8762
                     /gene="ompC"
                     /locus_tag="QBF46_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDQ6675744.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8874..9929
                     /gene="apbE"
                     /locus_tag="QBF46_003611"
     CDS             8874..9929
                     /gene="apbE"
                     /locus_tag="QBF46_003611"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDQ6675745.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            10003..11067
                     /gene="ada"
                     /locus_tag="QBF46_003612"
     CDS             10003..11067
                     /gene="ada"
                     /locus_tag="QBF46_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDQ6675746.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            11067..11717
                     /gene="alkB"
                     /locus_tag="QBF46_003613"
     CDS             11067..11717
                     /gene="alkB"
                     /locus_tag="QBF46_003613"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDQ6675747.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            11793..13436
                     /gene="yojI"
                     /locus_tag="QBF46_003614"
     CDS             11793..13436
                     /gene="yojI"
                     /locus_tag="QBF46_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDQ6675748.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKYALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13654..15300
                     /gene="mqo"
                     /locus_tag="QBF46_003615"
     CDS             13654..15300
                     /gene="mqo"
                     /locus_tag="QBF46_003615"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDQ6675749.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMEPNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPKPVQKEVADIAL"
BASE COUNT         3769 a         3709 c         4084 g         3872 t
ORIGIN      
        1 tgatatagcg ctgaaggcgc gtaggtgatt atcgcatcca gcattttgtt gtaataacca
       61 gcaccgatgc cagggaaggc gtgagtgata ttttcggtaa tgggggcaag ttctgcattt
      121 aatgcgcgga tacgctcctc acgtggtaag tcgatgtaga gatcatagcg atcgcctagt
      181 gcctgattaa gcaggttgac cacggcagaa agttttttct ctttttcaga taagactgct
      241 gaacgtcctt ccgtttctac gatataacca atagtaagcg ttgggacaat gaccatcagg
      301 attgccatca ggatcatttg attgcgtaag cggcgtggat aaatccactt catataatgc
      361 atgctgaaat ccactagtct tgtccggtat atgacgatta tcagaggtta aggtgatgat
      421 ttctcggcgg tgtatcatat tccagagaag agagaacatt gcggtaacac gcttttaccg
      481 ctaccttaac cacactccat cggtcacctg aggcggagct tcgccccttt gaaatacctt
      541 gcttcttttc gtacaaccct gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg
      601 ctctggctgt tgattgcttt ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga
      661 gaatcggaaa ttcgtcagga atttaatctg agttccgatc aggctcagcg ctttattcaa
      721 cgcacctctg atgtgatgaa agagctgaag tacatcgccg aaaatcgctt atcggcagaa
      781 aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg
      841 ctgtttgccg actccgattg ttccgcaatg agtaacacct ggcgaggttc tctggagtca
      901 ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg cggcttacga tctaaaccgg
      961 gtatttttaa tcggcagcga taacctctgc atggccaatt tcggtctgcg tgatatgcca
     1021 gtggaacgcg ataccgcgtt gaaagctttg catgaacgca tcaataaata tcgaaatgca
     1081 ccacaagatg atagcggcag taacctctac tggatcagcg aaggtccgcg ccctggcgtc
     1141 gggtattttt acgcgttgac gccagtttat ctggcgaacc ggttgcaggc gcttttgggt
     1201 gtcgagcaga ccatccggat ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt
     1261 accattcttg atgaaaatgg tcataccctg atttcgctta ccggaccaga aagtaaaatt
     1321 aagggcgatc ctcgctggat gcaggaacgc tcctggtttg gctatacgga agggttccgg
     1381 gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg
     1441 gttgataagg tgctggaacg cattcgcatg ttgatcctta acgcaatttt gctgaatgtg
     1501 cttgccggag ctgcattgtt tactctcgca cggatgtacg agcgacgtat tttcattccg
     1561 gcggaaagcg acgccctgcg actggaagaa catgagcagt tcaatcgcaa gattgtcgcc
     1621 tccgcgccag tgggtatctg cattttgcgt accgctgatg gcgtcaatat tttaagtaac
     1681 gaactggcgc atacctatct caatatgctt acgcatgagg accgccaacg actgacgcaa
     1741 attatctgtg ggcagcaggt caattttgtt gatgtcctga ccagcaacaa taccaatctg
     1801 caaatcagct tcgtccattc gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg
     1861 gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg agatggcaca agcagcggaa
     1921 caggcgagcc agtcaaaatc gatgttcctt gccaccgtca gtcatgagct gcgaacgccg
     1981 ctgtatggca ttatcggtaa cctggatctg ttgcaaacca aagagttacc gaaaggcgtc
     2041 gatcggctgg tgacggcaat gaacaactct tccagcctgt tgttgaaaat tatcagcgat
     2101 attctcgatt tctcgaagat tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca
     2161 ccgcgtgaag tgatgaacca catcaccgcc aactatttac cgctggtggt acgcaagcag
     2221 ttaggcttgt actgctttat tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg
     2281 cgtttacagc aggtcatctc caacctgttg agtaacgcca taaaattcac cgataccggc
     2341 tgtatagttt tgcatgtccg cgctgatggc gattatctct ctatccgtgt tcgcgatacc
     2401 ggcgtgggga ttccggcgaa agaagtggtg cgcttgtttg atcccttctt ccaggtcgga
     2461 acgggcgtac agcgaaactt ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg
     2521 atcagcatga tggacggcga tatctcggta gattcagaac cgggaatggg cagccagttt
     2581 accgtgcgta ttccgttgta cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg
     2641 agtggtaaac gctgctggct gtcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc
     2701 agtttgcagc gcagcggcat cgtcgttaca acatacgaag ggcaggaacc gactcccgaa
     2761 gatgtgttaa ttactgacga ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc
     2821 ttctgtcgtc gccatattgg tattccgctg gagaaagcgc caggggagtg ggtacacagt
     2881 gtggctgccc cgcatgagct accggcattg ttggcgcgta tttatttgat cgagatggag
     2941 agcgacgatc ctgctaacgc tctgccgtcg acggacaaag cggtcagcga taatgacgat
     3001 atgatgattc tggtcgtgga tgatcatccg attaaccgac gtctgctggc agatcagttg
     3061 ggatcgttgg gctatcaatg taaaaccgcg aatgatggtg tcgatgcgct taatgtactt
     3121 agcaagaatc atattgatat cgtgcttagc gacgtcaaca tgccaaatat ggatggttac
     3181 cgcttgacgc aacgcattcg tcagttggga ctgacgttgc cggtaatcgg agtaactgct
     3241 aatgcgttgg ctgaagagaa gcagcggtgt ttggagtccg gtatggacag ctgcctgtcg
     3301 aagccggtaa cgctggatgt gataaaacag acgctgacgg tatatgccga gagggtcagg
     3361 aaatcgcggg aatcgtagga cggataaggt gtttatgccg catctggcat tcagtgcaaa
     3421 tgccagatgc gacgctgacg cgtcttatct ggcctacttt aatgtaatat gttgaaataa
     3481 tgggaatcgt aggccgcata aggcgtttac gctgcatccg gcaatcggtg caaatgccag
     3541 ataagacact aacgcgtctt atctggccta cagatgatta gtctttatct gccggactta
     3601 aggtcacaga agagagataa ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca
     3661 tcgcagattt cttctggcta ctgatggttt taatactgcg gttcagcttt ttagcgatct
     3721 cggtcaccag gaagccttcc gcaaacaggc gcagaacttc actctctttt ggcgagagac
     3781 gcttgtcacc gtaaccacca gcactgattt tttccaacag gcgagaaacg ctttccgggg
     3841 taaatttctt ccctttctgc agcgcggcga gagctttcgg cagatcggtc ggtgcacctt
     3901 gtttcagcac gatcccttcg atatccagat ccaataccgc actaagaatc gccgggttgt
     3961 tgttcatggt cagaacaatg atcgacaggc ttgggaaatg gcgcttgatg tacttgatta
     4021 aggtaatgcc atcgccgtac ttatcgccag gcatggagag atcggtaatc aacacatgcg
     4081 catccagttt cggcaggttg ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat
     4141 tcacccactc aatttgctca agtgatttgc gaataccgaa caagactatc ggatggtcat
     4201 cggcaataat tacgttcata ttgttcatgt aataggctac cttgctacag caagctcttg
     4261 acataactgt caatgtcgct gatgtatttt tctattcctg gaacatcctt ctcacgaatc
     4321 agatgttcca gcgtttcaca taactgcttg ccgggtacca gatttagcat ggcaaatacg
     4381 cctttaagac gatgagccgt ttgggctaac gcagcaaagt cactggttgc tgcttcagta
     4441 tacagcctct taacatcatc cggtactgtg tctacaaaga gcgcataata gccgctggca
     4501 tggagttgcg cattttcatc tccgcccaga ggcgattctg tcacctcttc ctgcgccagt
     4561 tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg
     4621 cacaattgac caggcccaat ttcccgtacg ccagactcat catcgcttaa aagcaagcca
     4681 gaggcagtaa gattagacgg attatccgtt aaaaagatat cataatcttg actaattaat
     4741 ctttcatcgg gtgtgataca ggttgcaccc caattttcta actggcgagt gacaatattc
     4801 cgaatttctg ccgaagtaac atccaccatt acgcagacat catccagtaa acgctcttct
     4861 tcctcttcaa cttccgggtc agctgcgagc attttgatat gcacagagta gcgtgtacca
     4921 agcccatccc gcgttttgat gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc
     4981 agccagaatg ccagcgggtc cgccttgcca tagcgatcgt tttgggtctg gttgataaac
     5041 gggaagtgca aattatccat ttcatgaatg cttacgcctt ctccggtgtc cagaatgcgg
     5101 aacgtcaggc ggtcttcgga ggactcatcc tgatcaacct caagggtgat ttttcccaat
     5161 tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga
     5221 tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa tcagcagttg cagaccttta
     5281 cgcttgatgg caggcaacac tgaagggaca acttcatcaa ttaaatcctg cacggagaac
     5341 agcaccgtct cacttttcca gctatcgtcc gcaagcatgt tcgctaactg tatttcatcg
     5401 accagccgca ccagcacatc tgcctgattc gccagttgtt tgctttccgg ggcattgagt
     5461 ttagccgcgc tctccgccag ggactgtgcg ggttctttca gcgcatcgcc aatgtttttc
     5521 ataaaggtca tccgcccctg ctggtttttc tcatacagac gctgcgcctg cttgagtttc
     5581 ttgtttacca gcacttcgcg atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg
     5641 acctggctgc ggaacatgcg gatctcatac agctcgttat tgatcgtcgc ctgaataatc
     5701 ccctgatgct gttccgccat ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga
     5761 tcggcaattt tgttacttat gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg
     5821 agcggcagca gtgagactat ctcttcattg attgcccgta aaatgcgcaa ttcgttattg
     5881 accgccgtgc tggggacgtt ttctgtactg cggctggaga aatggcggaa tgtggtatag
     5941 ccaaataacg ccagcgccag caaaccgatg ttcagcagca gtggcagcag aatgttttgc
     6001 aacgtatcca gcaataaggt gccataagga acctgccaga ccaggcgcat atcggtagag
     6061 ttgagtgccg aggagatttc aatcttcgta ctgttaaagt ggatactgac gctatccgtc
     6121 ccttctttct cattatcatt gtttcccgtt gccgtcgcgt ctggctcaag gcggaaactg
     6181 tccagcggca tacccggtgg gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt
     6241 gccagatgtc ctggctggtt aaaggtagta cgcaatgtaa agtaatgacc gttctgccag
     6301 gccaggcggc gcatgttaga aaagctttcg cgttcatcga gggcgttggc ctgttgcaac
     6361 atctctgcac gacgtgaatc aacaatgtcg ctgacggtcg attctttaaa tccggaggtg
     6421 agatctttaa ggggtagggt tgagatcagc accagactgt tatcctgacc attcaggtaa
     6481 tacatcgacc acggtacatt ttctgcgccc cacaatgtat ccagataagt ggacatccgc
     6541 tgagtcatct caagcgttga gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg
     6601 cgtggtttct ccagatagta gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg
     6661 gaggaggggg aggtcgtcgc ggcaatgttg tcgtagatct gccaggtcac gtaacgccag
     6721 ttatcgacgc gcttttggat agcatgggta atgtcgacaa tctggtaact tttatctttc
     6781 agccaggcgt taacggcgct ttgtaccatt acacccatcg tcaccagtaa cacaatgatc
     6841 aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt
     6901 gtctctttct gacgcattcg tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag
     6961 tataaagggt acagtgtgaa attccagact cttacctgat tgatgattct ctattctccc
     7021 gtgatattta tcatcctgca ttgggggaaa tagatgtaca agcgccattt tgctgtacaa
     7081 ataacctaca aaaagcccaa cgaaattaat tgcaaataaa cgaagattgc tggaaattat
     7141 gctgatgtta attatttgtg aaatagttaa caagagttat agtttttctg tggtagcaca
     7201 gaataatgaa agtgtgtaaa gaagggtaaa aaaaaaccgg atgcgaggca tccggttgaa
     7261 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
     7321 cgggagatat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
     7381 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt
     7441 cgccattccg caataatctt aaaaagttcc cttgcattta cattttgaaa catctatagc
     7501 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
     7561 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
     7621 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
     7681 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
     7741 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacaacaagt
     7801 cagaagacgg cgaccagacc tatgtacgtc ttggtttcaa aggcgaaact caggttactg
     7861 accagctgac cggttacggc cagtgggaat atcagatcca gggcaatacc tctgaagaca
     7921 acaaagaaaa ctcctggacc cgtgtggcat tcgcaggtct gaaattccag gatgtaggtt
     7981 ctttcgacta cggtcgtaac tacggcgttg tttacgacgt aacttcctgg accgacgtac
     8041 tgccagaatt cggtggcgac acctacggtt ctgacaactt catgcagcag cgtggtaacg
     8101 gcttcgcgac ctaccgtaac actgacttct tcggtctggt tgacggcctg aactttgctg
     8161 ttcagtacca gggtaaaaac ggtagcgtaa gcggcgaagg catgaccaac aacggtcgtg
     8221 gtgctctgcg tcagaacggc gacggcgttg gcggatctat cacttatgat tacgaaggct
     8281 tcggtatcgg tggtgcgatc tccagctcca aacgtactga tgatcagaac agcccgctgt
     8341 acatcggtaa cggcgaccgt gctgaaacct acaccggtgg tctgaaatac gacgctaaca
     8401 acatctacct ggctgctcag tacacccaga cctataacgc aactcgcgta ggttccctgg
     8461 gttgggcgaa caaagcacag aactttgaag ctgttgctca gtaccagttc gacttcggtc
     8521 tgcgtccgtc tgtagcatac ctgcagtcta aaggtaaaaa cctgggtacc atcgctggtc
     8581 gtaactacga cgacgaagat atcctgaaat atgttgatgt tggtgctacc tactacttca
     8641 acaaaaacat gtccacctac gttgactaca aaatcaacct gctggacgac aaccagttca
     8701 ctcgtgacgc tggcatcaac actgataaca tcgtagctct gggtctggtt taccagttct
     8761 aatctcgatt gatatcgaac aaagggcctg cgggcccttt tttcattgtt ttcagcgtac
     8821 aaactcattt ttttggtgta ctcttgcgac cgttcgcatg aggataatca cgtatggaaa
     8881 taagctttac ccgcgtggca ctgctggctg ccgcgctctt ctttgttggt tgcgatcaaa
     8941 aaccacaacc cgccaaaacc cacgctactg aagttaccgt tcttgaaggc aaaaccatgg
     9001 gtaccttctg gcgtgccagc atcccgggca ttgatgccaa acgcagtgcc gaacttaaag
     9061 aaaagattca gacccagctg gacgccgacg atcagctgct ttcgacctat aaaaaagatt
     9121 ccgcgctgat gcgctttaac gactcgcaaa gtttgtcgcc gtggccagta agtgaagcga
     9181 tggccgaaat cgtcactacc tcgctgcgca ttggcacgaa gaccgatggc gcgatggata
     9241 taaccgtcgg gccgctggtg aatctgtggg gctttggccc ggaacaacag ccggttcaaa
     9301 ttccgagcca ggaacagatc gatgcgatga aagccaaaac cggcttacag cacctgacgg
     9361 tcattaatca gtcgcatcag caatatctgc aaaaagacct gccggattta aatgtcgatc
     9421 tctctaccgt cggcgaaggt tatgcggcgg atcacttggc acgcttgatg gagcaggaag
     9481 ggatttcccg ctatctggtg tcggtgggcg gcgcgctgaa cagccgtggt atgaacggtg
     9541 aaggccagcc gtggcgggta gcgattcaaa aaccaaccga taaagaaaac gcggttcagg
     9601 ctgtggtgga tatcaacggt catgggatta gcacctctgg cagctaccgt aactattacg
     9661 aactggacgg caaacgtctt tcccatgtta tcgatccgca aaccgggcgt cccatcgaac
     9721 acaatctggt atccgtgacg gtgattgctc cgacggcgct ggaagccgat gcctgggata
     9781 ctggcttgat ggtactcggg ccggagaaag ccaaagaggt tgttcgccgg gaagggctgg
     9841 cggtttatat gatcaccaaa gaaggcgata gctttaaaac ctggatgtca ccacagttta
     9901 aaagcttcct tgtcagcgaa aaaaattaaa gcgcaagatt gttggttttt gcgtgatggt
     9961 gaccgggcag actaaaggct atccttaacc agggagctga ttatgaaaaa agccacatgc
    10021 ttaactgacg atcaacgctg gcaatctgtc ttagcccgcg acccgaatgc cgacggcgaa
    10081 ttcgttttcg ccgtgcgtac cacaggcatc ttttgccgtc cgtcttgccg cgccagacat
    10141 gctttgcggg aaaacgtctc cttctacgca aatgccagcg aggcactcgc cgctggcttt
    10201 cgcccctgca aacgttgtca gccagaaaaa gccaatgccc agcaacatcg gttggataaa
    10261 atcacccacg cgtgtcgact gctggaacag gaaacgcctg taacgttgga agccttagcc
    10321 gaccaggtgg cgatgagtcc atttcatcta catcggttgt ttaaagcgac taccggaatg
    10381 acgcctaaag cctggcaaca ggcctggcgc gctcgccgtt tgcgcgaatc gctggcgaaa
    10441 ggggagagcg tgacgacgtc tattcttaac gccggattcc ccgacagcag cagttactat
    10501 cgcaaagctg acgaaacgct gggcatgacg gctaaacaat tccgtcacgg tggcgaaaat
    10561 ctggcggtgc gttacgcgct ggctgattgt gagctgggtc gttgcctggt ggcagaaagc
    10621 gagcggggga tttgcgcgat attgctgggc gatgatgacg cgacactaat cagcgagttg
    10681 cagcagatgt ttcccgctgc cgacaacgcg cctgccgatc tgatgtttca gcaacatgtg
    10741 cgtgaagtga tcgccagcct caatcaacgc gatacgccgc tgacgttacc gctggacatt
    10801 cgcggcactg cttttcagca acaagtctgg caggcactgc gcacgatacc ttgcggtgaa
    10861 accgtcagtt atcagcaact ggctaacgcc atcggcaaac cgaaagcggt acgggccgtt
    10921 gccagcgcct gtgccgccaa caagctggct atcataatac cctgtcatcg ggtggtccgt
    10981 ggtgatggca cactttccgg ttaccgctgg ggcgtgtcgc gtaaagcgca actgctgcgc
    11041 cgcgaagctg aaaatgagga gaggtaatgt tggatctgtt tgccgatgct gaaccgtggc
    11101 aagagccact ggcggctgga gcggtaattt tgcaccgttt tgcttttaac gctgcggagc
    11161 aactaatccg cgatattaat gacgttgcca gccagtcgcc gtttcgccag atggtcaccc
    11221 ccgggggata taccatgtcg gtggcgatga ccaactgtgg gcatctgggc tggacgagcc
    11281 atcggcaagg ttatctctat tcgcccattg atccgcaaac aaataaaccg tggcccgcca
    11341 tgccacagag ttttcataat ttatgtcaac gtgcggctac ggcggcgggc tatccagatt
    11401 tccagccaga tgcttgtctt atcaaccgct acgctcctgg cgcgaaactg tcgctgcatc
    11461 aggataaaga cgaaccggat ctgcgcgcgc caattgtttc tgtttctctg ggcttacccg
    11521 cgatttttca atttggtggc ctgaaacgta atgatccgct caaacgtttg ttgttggaac
    11581 atggcgatgt ggtggtatgg ggcggtgaat cgcggctgtt ttatcacggt attcaaccgt
    11641 tgaaagcggg gtttcatcca ctcaccaccg actgccgcta caacctgaca ttccgtcagg
    11701 caggtaaaaa agaataaaaa taagaattat tattgctgtg cgcgcgaaga tgtttaaact
    11761 gcgggctgta attcattgtc cgggttttct gcatggaact tcttgtactt gtctggcggc
    11821 agtatcgctg gccatttatc agtgtgatgg cgctaagcct cgccagtgcg gcattaggca
    11881 ttggcttaat tgcttttatc aatcagcgcc ttatcgaaac ggcggatacc agtctgctgg
    11941 tgttgccgga gtttcttgga ttattactgc tgttgatggc agtcactctc ggatcgcaac
    12001 tggcgctcac cactttgggg catcacttcg tttaccgact gcgtagtgaa tttatcaagc
    12061 ggattctgga tacccacgtc gagcgcattg aacaactcgg tagcgcctcg ttgctggcgg
    12121 gattaaccag cgatgtgcgc aatatcacca ttgcttttgt gcgtcttccg gaactggtgc
    12181 aggggatcat tctcactatc ggttccgcgg cgtatctgtg gatgctgtcg ggcaaaatgt
    12241 tgctggtaac ggctatctgg atggcgatca ccatctgggg tggttttgta ctggtggcgc
    12301 gggtgtacaa acatatggcg accctgcgtg aaaccgaaga caagctgtac acggattttc
    12361 aaaccgtact cgaagggcgc aaagagctga ctctgaaccg ggaacgcgcc gagtatgtgt
    12421 ttaacaacct ctacatcccc gatgcgcaag agtatcgcca tcatattatt cgcgcagaca
    12481 ccttccatct tagcgccgtg aactggtcaa acatcatgat gctgggcgct atcggcctgg
    12541 tgttctggat ggcgaacagt ctcggttggg ctgataccaa cgttgctgcg acctattcgt
    12601 tgacgctgtt attcctgcgt acgccgctgc tttcggcggt tggcgcattg ccgacgctgc
    12661 tgacggcgca ggtggcgttt aacaagctga acaaatacgc gctcgcgcct ttcaaagctg
    12721 agtttccgcg cccgcaggct tttcccaact ggcaaacgct ggagctgcgt aacgtgacgt
    12781 ttagctatca ggataacgcg ttttccgttg ggccgattaa cctcaccatt aaacgtggcg
    12841 agctgctgtt tctgattggt ggcaacggta gtggaaaatc gacgctggcg atgttgctga
    12901 cgggcttgta ccagccacaa agcggcgaaa tcttgctgga tggcaaacct gtcagtggcg
    12961 aacaaccgga agattatcgc aaactgtttt cggcagtgtt taccgatgtc tggctgtttg
    13021 atcaactgct ggggccggag ggtaaacccg ctaacccgca actggttgag aagtggctgg
    13081 cgcagctgaa aatggctcat aagcttgagt taagcaacgg gcgtattgtt aacctgaagt
    13141 tatcaaaagg gcagaaaaaa cgcgtggcgc tgttgctggc gctggcagaa gaacgcgata
    13201 ttatcctgct ggatgaatgg gcggcggatc aggatccaca cttccgtcgt gagttttatc
    13261 aggtgttgct gccgctgatg caggagatgg gtaaaactat tttcgctatc agtcatgatg
    13321 atcattactt tatccacgcc gaccgcctgc tggaaatgcg caatgggcaa cttagcgagc
    13381 tgacgggcga agagcgcgat gccgcttcgc gtgatgccgt tgcccggacg gcataatatt
    13441 acgacgctga cctggcggac tgctgccgtc aggtcaatat gctaaaaatc ccttcatttc
    13501 taataaccct ataattaatc aacgaaatta taatgtttct aaaattagaa tataatttat
    13561 aaacattatt taaatgttgt tacttaagtg ttaccgttga tgccgcgcaa atctcacctg
    13621 caataaagcg actaaaagta aggcattaac aagatgaaaa aagtgactgc catgctcttc
    13681 tcgatggccg tggggcttaa tgccgtttcg atggcggcaa aagcgaaagc gtccgaggag
    13741 caggaaactg atgtactgtt gattggcggc ggcattatga gcgccacgtt ggggacctat
    13801 ttacgcgagc tggagcctga atggtcgatg accatggtgg agcgcctgga gggtgtcgcg
    13861 caggagagtt cgaacggctg gaataacgcc ggaaccgggc attctgcact gatggaaccg
    13921 aactacaccc cgcaaaacgc cgatggcagc atcagtattg aaaaagcagt cgccattaac
    13981 gaagcatttc agatttcccg ccagttctgg gcgcaccagg ttgagcgcgg cgtgctgcgt
    14041 actccgcgtt catttatcaa taccgttccg catatgagct ttgtctgggg cgaggataac
    14101 gttaatttcc tgcgcgcccg ttacgccgcg ttgcaacaaa gctcgctgtt tcgcggtatg
    14161 cgttactctg aagatcacgc gcagatcaaa gagtgggcac cgttagtgat ggaagggcgc
    14221 gatccgcaac agaaagtggc agccacgcgt acggaaattg gtaccgatgt gaactacggc
    14281 gagatcaccc gccagttaat tgcttccttg cagaagaaat ctaacttctc gctgcaactc
    14341 agcagcgaag tccgcgccct aaagcgtaat gacgataaca cctggaccgt taccgttgcc
    14401 gatctgaaaa atggcactgc acagaacatt cgtgccaaat ttgtctttat cggcgcgggc
    14461 ggtgcggcgc tgaagctgtt acaggaatcg gggattccgg aagcgaaaga ctacgccggt
    14521 ttcccggtgg gcggacagtt ccttgtttcg gaaaacccgg acgtggttaa tcaccatctg
    14581 gcgaaggttt acggtaaagc atccgttggc gcaccaccga tgtcggttcc gcatatcgat
    14641 acccgcgttc tggacggtaa acgcgtagtg ctgtttgggc catttgccac cttctcaacc
    14701 aaattcctca aaaacggttc attgtgggat ctgatgagtt ccaccaccac ctctaacgtg
    14761 atgccgatga tgcacgtcgg gctggataat ttcgatctgg tgaaatatct ggtgagtcag
    14821 gtgatgttga gtgaagagga tcgttttgaa gcgttgaaag agtactatcc gcaggcgaaa
    14881 aaagaggact ggcgtttgtg gcaagcgggg cagcgcgtgc agattatcaa gcgtgatgcc
    14941 gagaaaggtg gcgtactgcg tctgggtact gaagtcgtca gtgaccagca agggaccatt
    15001 gccgcgctcc tgggggcatc gccaggggcg tcaaccgccg cgccgattat gttggatctg
    15061 ctggaaaaag tatttggcga tcgcgtttcc agcccgcaat ggcaggctac gttgaaagcg
    15121 atcgttccgt cttatggacg caagctgaac ggtgatgtag cggcaacaga acgcgagttg
    15181 cagtacacca gcgaagtgct ggggctgaaa tacgacaagc cgcaggcagc agatagtacg
    15241 ccgaaaccgc agttgaaacc gaaacccgtt caaaaagaag tggcggatat tgcgttgtaa
    15301 tgatgtgcca catccggcat ggtatgccgg ggatgtggcg tatgctgata agacgcgcca
    15361 gcgtcttatc aggcaaccgg ctcgggcgtt agatgtcgga tgcggcgtaa ccgccttatc
    15421 caacctacgg ggtg
//
LOCUS       DAOFQD010000112        15287 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.112, whole genome shotgun sequence.
ACCESSION   DAOFQD010000112 DAOFQD010000000
VERSION     DAOFQD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.112"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..979
                     /locus_tag="QBF46_003616"
     CDS             <1..979
                     /locus_tag="QBF46_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDQ6675750.1"
                     /translation="VSLSALWRNYWGRSGNAKDYQFSYSNSWQRISYTFSASQSYDEN
                     DKEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQL
                     NYGIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWSGGI
                     NITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDPIIPHRENHLVLDIANSE
                     SETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNI
                     GVVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            992..1951
                     /locus_tag="QBF46_003617"
     CDS             992..1951
                     /locus_tag="QBF46_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDQ6675751.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNAFRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVVYN"
     gene            complement(1989..2888)
                     /gene="nhaR"
                     /locus_tag="QBF46_003618"
     CDS             complement(1989..2888)
                     /gene="nhaR"
                     /locus_tag="QBF46_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDQ6675752.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2954..4120)
                     /gene="nhaA"
                     /locus_tag="QBF46_003619"
     CDS             complement(2954..4120)
                     /gene="nhaA"
                     /locus_tag="QBF46_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDQ6675753.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            4706..4858
                     /gene="mokC"
                     /locus_tag="QBF46_003620"
     CDS             4706..4858
                     /gene="mokC"
                     /locus_tag="QBF46_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDQ6675754.1"
                     /translation="MKQHKAMIVALIVICITAVVAVLVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(4962..6092)
                     /gene="dnaJ"
                     /locus_tag="QBF46_003621"
     CDS             complement(4962..6092)
                     /gene="dnaJ"
                     /locus_tag="QBF46_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDQ6675755.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGCVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(6181..8097)
                     /gene="dnaK"
                     /locus_tag="QBF46_003622"
     CDS             complement(6181..8097)
                     /gene="dnaK"
                     /locus_tag="QBF46_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDQ6675756.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8474..8878
                     /locus_tag="QBF46_003623"
     CDS             8474..8878
                     /locus_tag="QBF46_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDQ6675757.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            8904..9617
                     /gene="msyB"
                     /locus_tag="QBF46_003624"
     CDS             8904..9617
                     /gene="msyB"
                     /locus_tag="QBF46_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDQ6675758.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            9766..10332
                     /gene="satP"
                     /locus_tag="QBF46_003625"
     CDS             9766..10332
                     /gene="satP"
                     /locus_tag="QBF46_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDQ6675759.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(10367..10954)
                     /gene="mog"
                     /locus_tag="QBF46_003626"
     CDS             complement(10367..10954)
                     /gene="mog"
                     /locus_tag="QBF46_003626"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDQ6675760.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(11069..12022)
                     /gene="tal"
                     /locus_tag="QBF46_003627"
     CDS             complement(11069..12022)
                     /gene="tal"
                     /locus_tag="QBF46_003627"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDQ6675761.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            12301..13731
                     /locus_tag="QBF46_003628"
     CDS             12301..13731
                     /locus_tag="QBF46_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDQ6675762.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFTLLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMRQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            13801..14577
                     /gene="yaaA"
                     /locus_tag="QBF46_003629"
     CDS             13801..14577
                     /gene="yaaA"
                     /locus_tag="QBF46_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDQ6675763.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDNFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(14730..15026)
                     /locus_tag="QBF46_003630"
     CDS             complement(14730..15026)
                     /locus_tag="QBF46_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDQ6675764.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
BASE COUNT         3823 a         3816 c         3645 g         4003 t
ORIGIN      
        1 tgtatcatta agtgcgcttt ggcggaatta ctgggggcga agtggaaatg ctaaagatta
       61 ccaattcagt tattccaata gctggcaacg catcagttat actttctctg ccagccaatc
      121 ctatgatgaa aatgacaaag aagaggagcg ttttaatctg tttatctcca ttcctttcta
      181 ctggggggat gatattgcca aaacacgtca ccaaattaac ttatcgaatt cgacctcatt
      241 ttccaaagat ggctattcct ccaacaatac tggaattact ggcatagccg gtgaacatga
      301 tcagttaaat tatggtatat atgttaatca gcaacaacaa aataatgata catcgcttgg
      361 tacgaattta agctggagaa ctcccatcgc cataatagat ggcagctata gtcattctaa
      421 aaacgcctgg caaagtggtg gaagtattag tagtggatta gttgtctggt ccggtggtat
      481 taatatcact aaccagttat ccgatacatt tgcaattctg gatgcgcctg gattagaagg
      541 cgcgcatatt aatggacaaa aatacaaccg aacaaacagc aaaggccagg ttgtttacga
      601 cccgattata cctcatcgtg aaaaccatct ggtacttgat atagcaaaca gtgaaagtga
      661 aacagaattg cagggcaatc gtcaaattat tgcgccttac cgtggagcag tttcttatgt
      721 gcagtttaca actgaccaac gtaagccctg gtatatacag gcactgcgtc ccgatggttc
      781 gccattaacc tttggctatg acgtactgga tctccaggaa aacaatattg gagtcgttgg
      841 ccagggtagt cgccttttta ttcgcgtaga tgaaattcca actggcataa aagttgctct
      901 caatgatgaa cagaatttat tctgtactat tacttttcaa cacgttatcg atgaaaacaa
      961 aacatatata tgccagtaaa aggagtttac tatgaagtgg cttttattaa tcaccttgag
     1021 tctatattct tttattgtgc aaagtgctcc ctgtggattg accaatgtcg gggaacaacg
     1081 tggaacatat atactcaagc cactgagtat gaaaggtaat ctcaccgccg aaaaaattga
     1141 aaacaccgga aatatcattg agtgtgcaga cttacaagag acgaccaacg ccattaaagt
     1201 tcatttttta acacagaacg cttttaggtt taaagtcgga agtcgtcatt atattattaa
     1261 tttccccttt gaatctgacg gtaaaaaaat agagcttcca ggaaggtcat cttataattt
     1321 agaagaaatg ctatctgcga taaatttcac cattccatac tctatcgcat ctgtatcaga
     1381 tagttccaac accgcagata tttttccagg aactcctttt cctttgttgt tcgaaattga
     1441 agtgagttca tgtacaggga ataatactaa ttgtaatagc gtccgcttta attataattt
     1501 aaatactatt ttacaggtaa atttaatgac ctgtggtttt gaagatcaat ccatcgacac
     1561 tggacgattt gtactgtctc gtatcaataa tgaaaattat cagtttcatc atataagatt
     1621 tgactgcatc cagggcgagc aaggtgaatt agtttttgaa cccagtgatg ttgaatatta
     1681 ctttgaacca gtgacgatac aggagtcagg aaccagcata ttaaaaaatg agttagaaaa
     1741 cagtgaggac ggtgcgggtc aagtaggctt tcaactctcc aatgatggta cgaatgaaat
     1801 ccagtatggt aaaagcaatt actactcttt tcagcatccc catgaaggaa gtaatcagat
     1861 tcctctattt attcgcccgc gtacatatgg aaataatgtc tcaagcgggc aaataatgag
     1921 ccgagtcaaa atcgtggttg tgtacaacta atctatctcc tccactttaa ctttggggga
     1981 gctgccgatt aacgcgccgc tggactaaaa agcgcagaat aatccgtatt gcagattcgc
     2041 tgtaccgccg ggtgctgaat catccgctca gcaaaaatag catggtactc ttccatcaca
     2101 ttctcgacgc gaccaatttc tacgacagtt ttatcggcat aaaagtcata tgcataaagc
     2161 gttggggcaa cgaagattgc attgtgcatt gcaccaaaag ctttcatcaa cgcggcatca
     2221 tcaaactcgc cgaggatttc tacgtttaat ccctgggagt taaaccagtt aagcaatttg
     2281 cgccctaaca ttgaacgtcg cccaggaatc aaaagtcgcc gttcttccag acaagccggg
     2341 aacggttttt ctggtggtgg atttgtacac cagaaactca cgccacattc gccaattctc
     2401 acggagaaca ggccttcctg ctgcgtagag tctatcggac agtcggaaat gatcatatcc
     2461 agtttatgct ggcttaattg ctccagtagc atttcgtggg tggattcgaa gcagcgaaga
     2521 tgaatcggct cgccttctac cactgcagca ttcagcacgc tactgaccag gcgtttggaa
     2581 agtgcatcag ccacgccaac gtcaaacaat aaattggatt ctttgcgata gttcacaata
     2641 tccagcattt cctggcttaa ggtgaacatt ttatcggcat agcgatagac cagttctccc
     2701 agctcgctgg gttcgagacc acgtcccttg cgcttaaata atttgccttg caggcgctct
     2761 tccagagccc ggatctgtcc ggtaatggtt tgtggagtta aataaagcgc ctccgctgcg
     2821 ccaaccacgg aaccttcttt atagacatgc cagaagtaat acaagtggtt gtaattgata
     2881 tgagacatgc tcatttctct ccctgataac aatgaaaagg gagccgttta tggctccccg
     2941 gtaaaccgtc ctgtcaaact gatggacgca aacgaacgcg taaccagctg tatccaatta
     3001 ccgccgaaga gatagaaccg accaggatac cgagtttcgc ccagttaatc agttctggat
     3061 ctacgctacc aaaggccagg ctggcaataa agatagacat agtaaaaccg ataccgcaca
     3121 ggatccccac cgccataatt tgctgataag tcgttccctc aggcagatgc gccagtttca
     3181 aacgcagcgc caaccagcag aacagactaa tccccagcgg tttgccaatc agcaagccag
     3241 cgatgatccc caatggcaga atggaggtca agccatccag cgtgacacct tgcagtgaaa
     3301 cgccagcatt agcaaatgca aacagcggca aaatcagata cgccacccac gggtgtaaca
     3361 catgctccag tcgcttcgct ggagaacgcc catgcttctc tttcaaagga ataaagaagc
     3421 cgacaattac ccccgccagg gttgcgtgaa cccccgattt caacaccgca gtccacaaca
     3481 ctacgccaac cagaatatag acgcccgtgc ggcgtacacc acacagattc aataccgcga
     3541 gtaccgcaat tgctacagcc gcgacgccaa gagaggccat cgataagtca ttagtgtaga
     3601 acaatgcgat gataatgatg gccccaagat cgtcgataat agccagagcc atcaaaaaga
     3661 tcttcagcgc taacggaaca cgacttccca acagcgccag cacaccaagt gcaaaggcaa
     3721 tgtcagtagc cgccgggatc gcccaccctt cgcgggtaat cggatcggca tagttaaagg
     3781 ccagatagag taatgccgga acaatcatcc caccaatagc ggcgataact ggaaatgcgg
     3841 cctggcgtaa gctggctagc gatccttgca tcagttcacg tttaacttcc agaccgacta
     3901 acaggaaaaa taccgccatc agcgcgtcat ttatccataa cagcatgttt ttgttgattt
     3961 cgagtgaacc aacccggagc tgaaccggcg tctccagaaa gtcgtgatac catccactgg
     4021 ttgcgccgct gttggccatc atcatcgcca ggatagcggc aatgataaga ataatgcctc
     4081 ccgaggcatc actgctaaag aatcgatgca gatgtttcac tttttatttc tctttcaggt
     4141 gaatagatcg tttttactat tttaccccgc acgaatcatc gttaaaatca gattatagtg
     4201 taggaataat tcgtttttta cgaaccattg aaggatttgt gccatcagcg atgagatgag
     4261 tttttgacgg ctgtaagcgc taacgggtga ccctaagccc tgataatcat agcttaaggt
     4321 caggatttct gagggattaa ttaggaaaag taataattaa tttcttgtca gtcggcgtta
     4381 tttgttagga caatatttca aaaataatcc agcttgtcgt caataattgc gataaagaga
     4441 taaattaaag ttaaataatt aaatcattaa aaaattacgc cacaaaaatg cgaggcgtct
     4501 tcaggattag aatcgataaa acagaatggg ttaaagagag cgatataata gcggcgggtg
     4561 cttgaggcta tctgtctcag gcattagctg aacggcagat agagaaaagc cccgagtgat
     4621 attttactat caacccgagg ccccctatat gctgaacaca tgtagagtgc ctcttactga
     4681 ccgtaaggtc aaggagaaga gagcaatgaa gcagcataag gcgatgattg tcgccctgat
     4741 cgtcatctgt atcaccgccg tagtggcggt gctggtaacg agaaaagacc tctgtgaggt
     4801 tcacatccga actggccaga cggaggttgc tgttttcacg gcttacgaat ccgagtaaga
     4861 gcaacggcgg ggagtgatcc ccgccacttt tcaggtgctc gcatatcttc aacgcaccct
     4921 aattttaccc aagcctgccc acgggcaggc ttttggggag gttagcgggt caggtcgtca
     4981 aaaaacttct tcacaccatc aaagaagctc tttgagcgcg ggctgttgtg ctcaccggtt
     5041 gggccaccga agctttcttg cagctcttgc agcagctgtt tctgcttctc gttcaggcct
     5101 accggtgttt cgacgacaac gcggcacagt aaatcaccct gtgcgccacc gcggacagac
     5161 ttgacgcctt taccgcgcat acggaacagc ttaccagtct gggtttcgcc aggcactttc
     5221 agtttgacgc aaccatcaag ggtcggtact tcgatttcgc cacccagcgc cgccatagcg
     5281 aagttgatcg ggacttcgca atacaggttg ttgccttcac gctcgaaaat cgggtgctgt
     5341 ttaacctgaa cctgaacgta cagatcgcct gccggtgcgc catgctcgcc cgcttcacct
     5401 tcgcccgcaa gacggatgcg gtctccagtg tccacccctg ccgggatttt aacggacagc
     5461 gttttgctgc gctcaacacg accatgacca tgacatttgt tgcacggatc tttgatcagc
     5521 gtaccgcggc cctgacagtg tggacaggtc tgctgcacgg caaagaaacc ctgacgcatc
     5581 tgcacctgac cagaaccatg acaggtcgga caggtctgcg gctgtgtacc tggttttgca
     5641 ccgctaccgt ggcaaacgtc acactcttcc agagtcggaa tgcggatctc tttggtcacg
     5701 ccacgtacag cttcttcgag ggtgagctcc atgttatagc gtaaatcagc accgcgcgcc
     5761 gcacgttgac gaccacgtcc gccgccaaaa atatcgccga aaacgtcacc aaaaatatcg
     5821 ctgaagtctg cgccgccgcc aaaaccgccg ccgcccatac cgccttgctc aaacgcagca
     5881 tgaccatact gatcatatgc cgcacgtttt tgcgagtcgg tcagaacttc ataagcttcc
     5941 ttgatctctt taaatttcgc ctcggcctct ttgtcaccct ggttacggtc cgggtggtat
     6001 ttcatggcca ggcgtttgta ggcctttttg atttcacgct cttccgctgt tttggaaacg
     6061 cctaaaatct cgtaataatc ttgcttagcc atctttttta aattgcccct agatgaatgc
     6121 acgggcggag aggaaattcc ccttcgcccg tgtcagtata attacccgtt tatagggcga
     6181 ttattttttg tctttgactt cttcaaattc agcgtcgaca acatcgtcat ctttcgcgtt
     6241 gtttgcagaa gcatcagcac cggcagtctg ctgctgggca tgttgctgct gggcgatttc
     6301 catcagtttc tgggaaacct gtgccagctc ctgcattttc gcttcgatag cggctttgtc
     6361 ttcacctttc agagcagttt ccagtgcagt cagtgcagac tcgatagcag ttttgtcgtc
     6421 agccggcagt ttgtcgcctg cttcttcaac ctgcttacgg gtgctgtgca gcagatggtc
     6481 gccctggttg cgagtctgta ccagctcttc aaacttacgg tcagcttcgg cgttagcttc
     6541 tgcgtcgcgt accattttct ggatttcatc ttcgttcaga ccagaagacg ccttgatggt
     6601 gatcttctgc tctttaccgc tgtttttatc tttcgcggaa acgtgcagga taccgtcagc
     6661 atcgatatcg aaggtaactt cgatctgcgg catgccgcgc ggtgccgggt tgataccatc
     6721 caggttgaac tgacccagag atttgttatc agccgcacgt ttacgttcac cctgcagcac
     6781 atggatggtt accgcagact ggttgtcttc agcggtagag aacacctggc tgtgcttggt
     6841 cgggatagtg gtgtttttcg cgatcagcgt cgtcatcaca ccgcccatgg tttcgatacc
     6901 cagagacagc ggggtaacgt ccagcagcag tacgtctttc acgtcaccag tcagaacacc
     6961 accctgaaca gcagcaccga ttgctacagc ttcgtccggg ttaacgtctt tacgcggctc
     7021 tttaccaaag aactcagcaa ctttcttctg aaccattggc atacgagtct gaccaccaac
     7081 gaggataacg tcgtcgatat cagatacgga caggccagcg tcctgcagtg caactttcag
     7141 cggctcaatg gaacggttta ccagatcttc aaccaggctt tccagtttcg cacgagtcac
     7201 tttgatgttc atgtgtttcg gaccggtcgc atctgcagtg atgtacggca ggttaacgtc
     7261 ggtctgctga gcggaagaca gttcgatttt cgctttttct gccgcttctt tcaggcgctg
     7321 cattgccagc ggatcgttgc gcaggtcaat gccctgatct ttcttgaatt cttcaaccag
     7381 gtagttgatc agacggctgt cgaagtcttc accacccagg tgggtatcac cgttggtagc
     7441 cagaacttcg aaggtttttt cgccgtcaac ttcgtcgatt tcgataatag aaatatcgaa
     7501 agtaccacca cccaggtcat aaaccgcgat agtacggttg ccagtacctt tgtccagacc
     7561 gtaagccagc gcagctgcgg tcggttcgtt gatgatacgt tttacttcca gaccagcgat
     7621 acggcctgcg tctttggttg cctgacgctg agcatcgtta aagtatgccg gtacggtgat
     7681 aacagcttca gttaccggtt cacccaagta atcttcagcg gttttcttca tttttttcag
     7741 cacttcagca gagatctgcg gcggtgccat tttctggcct ttaacttcga cccatgcgtc
     7801 gccgttatca gcagcaataa ttttgaacgg catgatggaa acatcacgct gtacttcttc
     7861 gtcctggaag cggcgaccaa tcaggcgttt aatcgcaaac agggtgtttt gcgggttcgt
     7921 cactgcctga cgtttagccg gctgaccaac cagagtttca ccatcctggg tataggcaat
     7981 gatagaaggc gtggtgcgat cgccttcggc gttctccagc acacgaggag tggtgccatc
     8041 cataatcgct acacaagagt tggtagtacc caggtcgata ccaattattt tacccatcta
     8101 aacgtctcca ctatatattc ggtcatcatg tggttgtgag tctgtaatag gggcgaaacg
     8161 tctggtttca actgcccaat ttcatttttt tgcagactca ctcactgcgg ttgactacta
     8221 aatggggtcg taacccacgt catcaagggg gagatgcaaa aattttttgt gcttttgcgc
     8281 agaaatttcg cgatatagca ggacaagacg tgaaactgac agcaatttta ttcatataaa
     8341 gaatgaatcg cagccaagac attcatcaaa aaattgtaat aaaaagaaaa cattacgcac
     8401 ctgaatcttc tctttatcag cagtaaacta gtgggtatcc atccccctac ctctccccac
     8461 taagagaatc cttatgaaat ccgtttttac gatttccgcc agcctggcga ttagtctgat
     8521 gctgtgctgc acggcgcagg caaacgacca taaaatcctc ggcgtcattg cgatgccacg
     8581 taacgaaacc aacgatctgg cgctgaaact tcctgtttgc cgaattgtta aacgcataca
     8641 actctctgcc gaccatggcg atttacagtt aagcggtgca tcggtttatt tcaaagccgc
     8701 ccgtagcgcc agtcagagcc tgaatattcc ttcagaaata aaagaagggc aaaccactga
     8761 ctggatcaac attaacagcg ataacgacaa taaacgctgc gtctcaaaaa tcaccttttc
     8821 cggtcatacg gtgaactcat cggatatggc cacgctgaaa attatcggcg acgactaacg
     8881 ccagaatata gaagccacaa aaaatgaatg ttaattacct gaatgattca gatctggatt
     8941 ttctccagca ttgcagtgag gaacagttgg caaatttcgc ccgattgctc acccataatg
     9001 aaaaaggcaa aactcgcctc tccagcgtac tgatgcgcaa cgaactgttt aaatcgatgg
     9061 aagggcatcc cgagcaacat cgccgcaact ggcagctgat tgctggagaa ttacagcatt
     9121 ttggtggcga tagtatcgcc aacaaactgc gcggacacgg taaattgtat cgggccattt
     9181 tgctcgatgt ttcaaaacga ttgaagctga aagccgacaa agagatgtct acgtttgaga
     9241 ttgagcaaca gttactggaa caatttctgc gtaacacctg gaagaatatg gacgaggaac
     9301 ataaacagga gtttctgcac gcggtcgatg cgagggtgaa tgagctggaa gagctgctgc
     9361 cgctgctgat gaaagacaaa ttattggcaa aaggcgtgtc gcatttgctt tccagccaac
     9421 tgacccgcat tttacgcacc cacgcagcaa tgagcgtact tgggcatggt ttgctgcgcg
     9481 gcgcggggct gggaggccct gtaggtgcgg cactaaatgg ggttaaagcg gtcagcggca
     9541 gcgcctatcg cgtgacgatt ccagccgtac tgcaaatcgc ctgcctgcgc cggatggtta
     9601 gcgccactca ggtctgatac cggaaaccgg ggaaaatttt cattttcccc tcaaaagatc
     9661 gtagacactg ccccactggc tgattattat gccgcgccct gaaaacacta cagttattca
     9721 gggaaattat ttcaccattc attcgatgat gatttttgag gaattatggg caacactaag
     9781 ttggctaatc cggcaccgct gggcctgatg ggcttcggca tgaccaccat tctgcttaac
     9841 ctgcacaacg tgggttattt cgctctggac ggtattattc ttgccatggg cattttctac
     9901 ggcggcatcg cgcaaatttt tgctggtctg ctggagtaca aaaaaggcaa cactttcggt
     9961 ttaaccgcat tcacctctta cggttctttc tggctgacgc tggttgcgat tctgctgatg
    10021 ccgaaactgg gtctgaccga tgcgccaaat gcacagttcc ttggtgtcta cctgggtctg
    10081 tggggcgtat ttacgctgtt tatgttcttc ggcacgctga aaggcgcacg cgttctgcaa
    10141 ttcgttttct ttagcctgac cgtgctgttt gccctgctgg cgatcggtaa cattgccggt
    10201 aacgccgcaa tcatccactt tgccggctgg attggtctga tctgcggtgc cagcgcaatc
    10261 tatctggcga tgggtgaagt actgaacgag cagtttggtc gcaccgttct gccgattggt
    10321 gaatcccact aattgttttg agatcccccc gctcgggggg atttttttat tcgctaacgt
    10381 cgcgtcttgc actcttcggt ctgaatgcag caaccacttc tggtgccgtt tcaacgtatg
    10441 gcccttccag caactgaatg cagtacggta cgctggcaaa aataccgtgt accacaacgt
    10501 tacccgcagc gtccttcaca ccttccagcg tctctttaat agacttcggc tgaccgggta
    10561 agttaaggat cagcgcctgt ttgcgaatca caccaacctg acgcgaaagg atcgcagttg
    10621 gtacaaaatg caggcttatc tggcgcatct gttcaccaaa gccaggcatc tcgcggtccg
    10681 ctactgccag cgtcgcatcg ggcgttacgt cacgacgcgc cgggccagtt ccgcccgtgg
    10741 tgagcaccag atggcaactc atttcatcca ccagctcaca caacgtttgc tcgatgatcg
    10801 cctgctcatc ggggattaag cgggtttcca gttcaaacgg cgtggttagc gccgatgtca
    10861 gccattcttc cagcgcaggg atgcctttat cctgataaac gccgctggat gcgcgatcag
    10921 agatggaaac taagccaata cgtaaagtat tcatcaagat ttttccgttt aaacagtctc
    10981 gttaaacaga atgatacact gcgaagggag tgacagacag gcttcagaag aggtagcgtg
    11041 accgacttcc cggtcacgct aagaatgatt acagcagatc gccgatcatt ttttccagtt
    11101 tttcctggtc aatagcaaac ttacggatac cttccgccag tttatctact gccattggat
    11161 cctggttgtg ctgccacagg aactcggact cagtgatacg cgccggacgc gctttcactt
    11221 cgccggtgta agacagttta cgttcgatag ccccttcgct ctccgccagc tctttcagca
    11281 gtgccggtgc gatggtcaga cggtcgcagc ctgccagttc cagaatttcg ccgatgttac
    11341 ggaagcttgc gcccataacc actgtttcat aaccgtgctc tttgtagtac tggtagattt
    11401 cagatacaga aaccacgccc ggatcttctg ccggagcgta ctctttctta tcagtattcg
    11461 ctttgtacca gtcaagaata cggccaacaa atggcgagat caggaacacg cctgcttccg
    11521 cacaagcacg agcctgagcg aaggagaaca gcagggtcag gttacagttg atgccttctt
    11581 tttccagctg ttctgcagca cggataccct gccaggtaga agccagtttg atcagaatac
    11641 gatcgttgct aatacctgca tcgttgtaga gtttgatcag gcgttttgct ttcgcaattg
    11701 acgcttcggt gtcataggaa agacgcgcat caacttcagt tgagatacgg cccggaacca
    11761 gtttcaggat ttccagacca atatttactg ccagtttgtc ggtcgcgtcc acgatctgct
    11821 gcgcgcgatc gttgctctgc tgtttcgccc aggcgacagc atcatcaatc aacttacggt
    11881 attccggaat ctgcgctgcg ttaagaatga gagaagggtt ggttgtggca tcctgcggtt
    11941 gatacagctt cattgccgcg atgtccccag tgtcggccac tacggtggtg tactgacgaa
    12001 gggaggtcaa tttgtccgtc atgatagtat ttctctttaa acagcttgtt agggggatgt
    12061 aaccggtctg ccctgatgat atcacgacgc aacgtcagcg caaccgccga caagacggtt
    12121 atcgccaggc gagactgttt cggatttctg acactgcgtt gatatcgcct gccattttta
    12181 tacaaaacct catgtatgct acgcagaagt tatcaagtac ctcgtagcgt atatacttct
    12241 taaacaattg gtaacgttta cacaggaaag tcatcgcgac cggcaataag agggatatgc
    12301 atgccagatt ttttctcctt cattaacagc gtcctttggg gatcggtaat gatttacctg
    12361 ctcttcggcg caggttgttg gttcactttt cgcaccggat ttgtgcagtt tcgctacatc
    12421 cgccagtttg gcaaaagtct taaaaatagc attcatccac agccaggcgg tttaacctca
    12481 tttcagtcat tgtgtaccag tcttgcggcg cgcgtgggta gcggcaacct ggccggcgtt
    12541 gcgctggcta ttaccgccgg tggacctgga gccgtctttt ggatgtgggt tgccgcgttt
    12601 atcggcatgg cgacctcgtt tgccgaatgt tctctggcac aactttataa agaacgtgac
    12661 gtcaatgggc agtttcgtgg cggaccggca tggtatatgg cgcgcgggct ggggatgcgc
    12721 tggatgggcg ttctgttcgc cgtctttttg ctcatcgcct atggcataat tttcagcgga
    12781 gttcaggcga acgccgttgc ccgcgccctg agtttttctt ttgattttcc cccgctggtg
    12841 acaggcatta ttctcgctgt ctttactctg ctggcaatca ctcgcggtct tcatggcgtc
    12901 gcccggctca tgcaggggtt tgtcccgttg atggcgataa tctgggtact gaccagcctg
    12961 gtgatttgcg taatgaatat cgggcaactt ccccacgtca tttggtctat ttttgagagt
    13021 gcttttggct ggcaggaagc ggcaggcggc gcggcgggat ataccttaag ccaggcgatt
    13081 actaacggtt ttcagcgcag tatgttttcc aatgaggcgg gaatgggttc gacgccaaac
    13141 gcggcagcgg cagcggcgtc ctggcctccg catccggcag cgcaaggaat tgtccagatg
    13201 attggcattt ttatcgacac cctggtcatc tgtacggcaa gcgccatgct gatattactg
    13261 gcgggtaacg acacaaccta catgccgctg gaaggtattc agcttatcca gaaggcgatg
    13321 cgggtgctaa tgggttcctg gggtgctgag tttgttaccc tcgtggttat tctgtttgcc
    13381 ttcagctcca tcgttgccaa ctacatttat gccgaaaaca atctcttctt tttacgcctg
    13441 aacaacccta aagcgatctg gtgtttgcgg atctgcacct tcgcaacggt catcggcggc
    13501 accttgctaa gtcttccgct gatgcggcaa ctggcagata tcataatggc ctgcatggct
    13561 attaccaatt tgaccgccat tttactgctc tcgcctgtgg ttcataccat tgccagtgat
    13621 tatctacgcc agcgtaaact cggcgtgcgc ccggtgtttg atccgttgcg ttatccggat
    13681 atcggtcgcc agctttctcc ggacgcgtgg gatgatgttt cgcaggagta atcacaacta
    13741 tcgatcaact cattctcttt ttttgctaaa gtcggcataa atttcctgca aggactggat
    13801 atgctgattc ttatttcacc tgcgaaaacg cttgattacc aaagcccgtt gaccaccacg
    13861 cgatatacac tgccggagct gttagacaat tcccagcagt tgatccatga agcgcggaaa
    13921 ctgacgcctc cgcagattag cacgctgatg cgcatcagcg acaaactggc gggtatcaac
    13981 gccgctcgct ttcatgactg gcagccagat ttcacgccgg agaatgcccg ccaggctatt
    14041 ttggcgttta aaggtgatgt ctacaccggc ttgcaggccg aaactttcag cgaagacaat
    14101 ttcgattttg cccaacagca tttgcgaatg ctttccggct tgtatggcgt actgcgcccg
    14161 ctcgatttaa tgcagcctta tcgtctggaa atggggatcc gtcttgagaa tgcccgaggg
    14221 aaagatctgt atcaattctg gggagatatc atcaccaaca agctgaacga ggcgctcgca
    14281 gcacaaggcg ataatgtggt gattaatctg gcgtcagatg agtattttaa atcggtgaag
    14341 ccgaagaaat tgaatgccga gattatcaag ccggtgttcc tcgatgagaa gaacggcaaa
    14401 tttaagatca tcagcttcta cgctaagaaa gcacgcggtc tgatgagtcg ttttattatt
    14461 gaaaatcggc tgaccaaacc agagcaactg actggtttta atagcgaagg ttacttcttt
    14521 gatgaagctt cctccagcaa tggagaactg gtgtttaaac gctacgagca gcgttaagtt
    14581 tgcagtcaat gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc atgcagattc
    14641 aatatattgc agaattcacg taggcctgat aagacgcgcc agcgtcgcat caggcgctga
    14701 atgccggatt gttacccggc atttgtcatt tagcggtgat gtttgcctgg accatgaccg
    14761 ccgtgatgat catgatgagc tttcttatgg tgacgcggcg gtggcggcgg tccgtgtggg
    14821 tgccagcgat tgcctcgcca ttcataatgt tgtttccacc agccgtggtc gcgccagtga
    14881 ccgccatccc agtaatagcc acgattatca cgatcgccta tctgtaattt tactgacggg
    14941 actaacgtaa tttccgcagc ctgcgctgcc atgggagcga ccagaaccag ggaaagtgcg
    15001 agtacgatag attgcatctt tttcacaggt tactcctttt atcattgccc gttatgggtc
    15061 gatatccgga gattacgcga tgagaacggc ggttgttatt ctccgcaatc ctaatatcta
    15121 agttaccgca tttattgaga atttctcctt tttccctgtc gtttctctcc atattttctt
    15181 cacaaaaaag ccgggctgca taaaagcaaa cccggcctga ttgagataat gaaaagatat
    15241 tactgatgat tcatcatcaa tttacgcaac gcagcaaaat cggcggg
//
LOCUS       DAOFQD010000113        14900 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.113, whole genome shotgun sequence.
ACCESSION   DAOFQD010000113 DAOFQD010000000
VERSION     DAOFQD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.113"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..511)
                     /locus_tag="QBF46_003631"
     CDS             complement(<1..511)
                     /locus_tag="QBF46_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675765.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIR
                     LEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPN"
     gene            complement(724..2394)
                     /gene="betA"
                     /locus_tag="QBF46_003632"
     CDS             complement(724..2394)
                     /gene="betA"
                     /locus_tag="QBF46_003632"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDQ6675766.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2408..3880)
                     /gene="betB"
                     /locus_tag="QBF46_003633"
     CDS             complement(2408..3880)
                     /gene="betB"
                     /locus_tag="QBF46_003633"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDQ6675767.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3894..4481)
                     /gene="betI"
                     /locus_tag="QBF46_003634"
     CDS             complement(3894..4481)
                     /gene="betI"
                     /locus_tag="QBF46_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDQ6675768.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKTRANSLTRHFITQHLPTD"
     gene            4610..6643
                     /gene="betT"
                     /locus_tag="QBF46_003635"
     CDS             4610..6643
                     /gene="betT"
                     /locus_tag="QBF46_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDQ6675769.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLLIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            6949..7023
                     /locus_tag="QBF46_003636"
     CDS             6949..7023
                     /locus_tag="QBF46_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675770.1"
                     /translation="MSDILLIVLNIVFIVIAIILVIIC"
     gene            7518..8606
                     /gene="pdeL"
                     /locus_tag="QBF46_003637"
     CDS             7518..8606
                     /gene="pdeL"
                     /locus_tag="QBF46_003637"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDQ6675771.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(8648..9580)
                     /locus_tag="QBF46_003638"
     CDS             complement(8648..9580)
                     /locus_tag="QBF46_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6675772.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(9672..10169)
                     /locus_tag="QBF46_003639"
     CDS             complement(9672..10169)
                     /locus_tag="QBF46_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDQ6675773.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGVISEYGGQKLSEATT
                     KRDGY"
     gene            10427..11032
                     /locus_tag="QBF46_003640"
     CDS             10427..11032
                     /locus_tag="QBF46_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDQ6675774.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            11072..11935
                     /locus_tag="QBF46_003641"
     CDS             11072..11935
                     /locus_tag="QBF46_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDQ6675775.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYLGLQRGKNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            11925..13472
                     /gene="fdrA"
                     /locus_tag="QBF46_003642"
     CDS             11925..13472
                     /gene="fdrA"
                     /locus_tag="QBF46_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDQ6675776.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIGSAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLDRGETPVDEQGLQFARGTKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIETI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            13472..14889
                     /locus_tag="QBF46_003643"
                     /pseudo
     CDS             13472..14889
                     /locus_tag="QBF46_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
BASE COUNT         3534 a         3742 c         3936 g         3688 t
ORIGIN      
        1 cgttcggata caatgaccag ttcctggtca ggtatatcat ttttgtataa agctcactgt
       61 taattgctga aggcgtttgt atctcatcga gaacttgcct gccattatga tattctgacg
      121 cataccattt gataatagag gcctgcacgc gagatatttc ctcttcagca gttggtaaag
      181 ctctcttatc tcctgaccat tccagcctta tttctctgtt cttgacatct gggctggtgt
      241 gaaaaaatgt agtaattcgc tgaataacat cttcagtcat tgtagtctga caggcaaaat
      301 tattattgct atcacagcta acaagtatac ctggcgttgt ctggtttatt tttaggctgt
      361 aaatcacttt tatattgtct ttattgatat acagaaacaa cagcaccata gccatacata
      421 tagccataat aaaaaggata acacgaatct ttttcttcag ataaaataaa atctcaatca
      481 gatcgatatc ctgtttgatt tgttcacgca taatatatcc agaaaataaa atctgtcgca
      541 gataaggttg tattaatagt ctgtatcagg aatgttcggg ttaaatatca gcaaaaagcc
      601 cgcatcatga atactggata tgaagcatga gagttacctc agtgtttata taaggattcg
      661 gtccccctct ctggaacggt aactctcaat ctgatcggtt cctgcgttag ttcacatcac
      721 gactcatttt ttcgctctca ccggcatccc atttgccaca aaatatcccg ccgtgctcct
      781 cggcagcgct tcctgtccac gaatcatatc cgctattttc tcgccaatca taattgtcgt
      841 ggcgttcaaa ttcccggtga taatctgcgg cataatcgac gcatccacca cacgcaggcc
      901 ttctaacccg tgtacgcggc cttcgccgtc aaccacggac atctcgtcgt aacccatttt
      961 gcaggtaccg cacggatgga aggcggtttc ggcgtggtta cgcacgaact catcgagctg
     1021 ttcatccgtc tggcattcga caccggggct gatttcgcgg ccacgatact gatccagcgc
     1081 gggttgatgc atgatctcgc gggtgatgcg aattgcgtcg cggaactcct gccagtcctg
     1141 ctcgtgcgac atgtagttaa acagaatcgc cggatgctgg tgcgggtcgc gggatttaat
     1201 ccgcacatgc ccacggcttg gcgagcgcat tgagccgacg tggcactgga aaccgtgctc
     1261 tttcactgca ttcgagccgt tatagttaat cgctactggc aggaaatggt actgaatatt
     1321 cggccacgca aattcctcac ggctgcgaat aaatccacct gcttcaaagt ggttgctggc
     1381 accaacgcca gtgccgccaa acagccactc cgcaccgatt ttcggctggt tccaccactg
     1441 cagggcaggg tagagggaaa ccggttcttt gcactcatat tgcagataca tctccagatg
     1501 atcctgaaga ttttcgccga cgccgggtaa ttcatgcacc agcggaatat caaactccgc
     1561 cagcagttca gcgttgccga cgccggagcg ttgcaggatc tgcggtgagg caatcgcgcc
     1621 tgcacataac agcacttctt tgttggccgt tgcgcgggtt gggatggtgc tgtcgccttc
     1681 cagccattcg acgcccaccg cgcgtttgcc gtcaaaaatg atgtgatcgg tcatagcgtg
     1741 agtacgaatg gtcaggttag gacgcgattt ggcctgatcg agatagccac gcgcggtgct
     1801 ggcgcgacgg ccctgcggcg tgacggtgcg atccatcgga ccaaaacctt cctgctgata
     1861 accgttgaga tcgtccgtgc gcgggtagcc cgcctgcacg cccgcttcaa tcatcgcttc
     1921 aaacagcgga ttgacgccgg gtttggaggt agtgacgctc accgggccat caccgccgtg
     1981 atagtcgttt tcacccatat cgcgagtctc ggccttgcgg tagtagggca ggcagtcgag
     2041 gtagctccag ttctccagac cgggttcttg cgcccagtta tcgagatcca gcgcattgcc
     2101 acggatgtag cacatgccgt tgatcagcga cgatccaccc agacctttac cgcgtccgca
     2161 ctccatgcgg cggttattca taaacggttc aggttccgtt tcataggccc agttgtagcg
     2221 tttaccctgt agcgggaatg ccagggcagc gggcatctgg gtgcggaagt caaagcgata
     2281 gtccgggccg cccgcttcaa gcagcagcac ggaggtattc ggatcttcag tcagacgggt
     2341 agcgagaacg ttgccggctg agccggcacc aataatgatg tagtcaaatt gcaaataaac
     2401 ctcctggtta gaatatggac tggaatttag ccatctcaac ctggatggac ttcacctggg
     2461 tgtaactctg gagcgtcatc acgccgttct cgcgaccaat gccggagtgt ttgtagccgc
     2521 caacgggcat ctctgccggg gattcgcccc aggtgttgat ccagcaaata cccgcttcca
     2581 gctgatgaat gacgcgatgc gcgcggttca ggtccgctgt cacgatgccc gccgccaggc
     2641 cgtagtcggt atcgttagcg cggcgaatga cttcgtcttc cgactcgtag gtcagaatgg
     2701 acatcactgg cccgaagatc tcttcacgca cgatggtcat atcgtcgctg caatcggtga
     2761 acactgtcgg tgcaacccat gcgccgttat cgaagccatc gcctttcagt acatcgccgc
     2821 cgcacagtac gcgcgcgcct tcctctttgc ctttggcgat atagcgcagc acgttatcgc
     2881 gatgcgggaa gctgaccagc gggccgaagt tagtttgcgg atcgaaaacg tcgcccgcgc
     2941 gaatgcgctc aacgcgcgcc agaattttct gctcaaatgc ggctttgcat ttcgccggaa
     3001 cgaagacgcg ggtgccattg gtacacacct gaccggagct gaagaagttt gccatcatgg
     3061 cgatatcggc ggcgagatcg agatccgcat catcgaaaac gatcagcggt gatttaccgc
     3121 ccagttccat ggtcacttct ttcagggaag aggccgccga gttagccatc acttttttgc
     3181 cgctggcgac accgccggta aatgacactt tggcaatgcc cggatgctcg gtcagatatt
     3241 gcccggtctc cgcgcccacg cccggcaaca cgttaaatac gccgtccggc aggcccgctt
     3301 cgctgtaaat ttcagccagc tttaacgcgg taagcggggt aacttcgctc ggtttgaaaa
     3361 tcattgcgtt gcctgccgcc agcgccgggg cggatttcca cagggcaatc tggatcgggt
     3421 agttccatgc gccaatccct gccactacgc ccagcggttc gcggcgggta tacacaaagg
     3481 acgtttcacg caacgggatc tggctgcctt ccagcgccgg gatcagcccg gcgtagtact
     3541 ccagcacgtc cgcaccggta acgatatcga cggttgaggt ttccgaatat gcttttccgg
     3601 tgtcgagggt ttccagtttt gcgagttcgt cattgcgttc acgcagaata tcaacggccc
     3661 gacgcagaat acgcgagcgc tccatggcgg tcatcgacgc ccagattttt tgcccctgct
     3721 gggcgctttt cacggcgcga tcgacatcct cgcgcccggc ggcctgcacg gtcgccagca
     3781 cgttaccgtt ggccgggtta atggtctcga aggtgcgacc gctggtggcg gaggtataac
     3841 caccatgtat ataaagctgc tgttctgcca ttcgggacat cacgtctcct cggttaatcg
     3901 gtgggtagat gctgagtgat aaagtggcgg gtcagggaat tagcgcgggt tttatccagc
     3961 ggtttgccgc tcagagccgc gcgcagccat aatccatcaa tcagcgcggc caggccgtag
     4021 cccgcttcct gtgcctgttc gcgcggcaat tcgcgacgaa actcgctcac cagattcgac
     4081 agcaagcggc ggctgctgac ctgctgtaaa cgatagagca tcggctgatg catactgctg
     4141 gcccagaacg ccagccaggc tttcatcgcc gcactgctca cctgcgtttc atcgaagttt
     4201 ccgccaacaa tcgcctgtaa tcgctgctct gcactgccct gcggaagtgc atgtaatcga
     4261 ttcaaaaccg cgtcacgcag ctgactggtg atatcgcgca tggttgcttc cagcagacca
     4321 tttttgtccc tgaaatagtg gctgatgatc cccgtagaaa cgcctgcacg gcgggcgatc
     4381 tgcgcgatcg ttgcatcgtg catgcccact tcatttattg cttccagtgt ggcgtcgatc
     4441 agttgtctgc gccggatcga ctgcatcccc aatttgggca ttttcgccac tccattcatc
     4501 agcggtgttt atctattaaa gcggttattg attggacgtt caatataaaa tgtgtcttaa
     4561 ttgttacgaa tttgatttta aatagtaaca ataacagtgg ggatactgga tgacagacct
     4621 ttcacacagc agggaaaagg acaaaatcaa tccggtggtg ttttacacct ccgccggact
     4681 gattttgttg ttttccctga caacgatcct gtttcgcgac ttctcggccc tgtggattgg
     4741 ccgcacgctg gactgggttt ctaaaacctt cggttggtac tatctgctgg cggcaacgct
     4801 ctatattgtc tttgtggtct gtatcgcttg ttcgcgtttt ggttcggtga agctcgggcc
     4861 agaacaatcc aaaccggaat tcagcctgct gagttgggcg gcgatgctgt ttgctgccgg
     4921 gatcggtatc gacctgatgt tcttctccgt agccgaaccg gtaacgcagt atatgcagcc
     4981 gccggaaggc gcgggacaga cgattgaggc cgcgcgtcag gcgatggtct ggacgctgtt
     5041 tcactacggc ttaaccggct ggtcgatgta tgcgctgatg ggcatggcgc tcggatactt
     5101 tagctatcgt tataatttgc cgctcaccat ccgctcggcg ctgtacccga tcttcggtaa
     5161 acggattaac gggccgatag gtcactcagt ggatattgca gcggtgatcg gcactatctt
     5221 cggtattgcc actacgctcg gtatcggtgt ggtgcagctt aactatggct tgagcgtact
     5281 gtttgatatt cccgattcga tggcggcaaa agcggcactg atcgccttgt cggtgatcat
     5341 cgccactatc tcggtgacat ccggtgtcga taagggcatt cgcgtgttat cggagcttaa
     5401 tgtcgcgctg gcgctgggat tgatcctgtt cgtgttgttt atgggcgaca cttcgttcct
     5461 gcttaatgca ctggtgctga atgttggcga ctatgtgaat cgctttatgg gcatgacgct
     5521 caacagtttt gccttcgacc gtccagttga gtggatgaat aactggacgc tcttcttctg
     5581 ggcatggtgg gtggcatggt cgccgtttgt cggcttgttc ctggcgcgta tctcgcgtgg
     5641 gcgtaccatt cgccagttcg tgctgggcac gttgcttatt ccgtttacct tcacgctgtt
     5701 atggctctcg gtgttcggca atagcgcgct gtatgaaatc atccacggcg gagcggcatt
     5761 tgccgaggaa gcgatggtcc atccggagcg cggcttctac agcctgctgg cgcagtatcc
     5821 ggcgtttacc tttagcgcct ccgtcgccac cattactggc ctgctgtttt atgtgacctc
     5881 ggcggactcc ggggcgctgg tgctggggaa tttcacctcg cagcttaaag atatcaacag
     5941 cgacgccccc ggctggctgc gcgtcttctg gtcggtggcg attggcctgc tgacgctcgg
     6001 catgctgatg accaacggga tatccgcgct gcaaaacacc acggtgatta tggggctgcc
     6061 gttcagcttt gtgatcttct tcgtgatggc ggggttgtat aaatctctga aggtagaaga
     6121 ttaccgccgt gaaagtgcca accgcgatac cgcaccgcga ccgctggggc ttcaggatcg
     6181 cctgagctgg aaaaaacgtc tctcgcgcct gatgaattat ccgggcacgc gttacactaa
     6241 acagatgatg gagacggtct gttacccggc aatggaagaa gtggcgcagg agctgcggtt
     6301 gcgcggcgca tacgtggagc taaaaagcct gccgccggaa gagggacaac agttggggca
     6361 tctggatttg ttggtgcata tgggcgaaga acaaaacttt gtctatcaga tttggccgca
     6421 gcaatactcg gtgccgggct ttacctaccg cgcacgtagc ggtaaatcga cctactaccg
     6481 gctggaaacc ttcctgttag aaggcagcca gggcaacgac ctgatggact acagcaaaga
     6541 gcaggtgatc accgatattc ttgaccagta cgagcggcac cttaacttta ttcatctcca
     6601 tcgtgaagcg ccgggccata gcgtgatgtt cccggacgcg tgattgttat tgcataaaac
     6661 cgcgccatgt ctgcatatgg cgcggtttct cttacctctt gatacgttat atctgtacgg
     6721 ttaagccctt agtatcgatt gatgattgcc aggcaacaga taataaaaaa gaaaagaact
     6781 attgcagccc aaaacctacg tttggactgt tgtgaatgtt caataagctt agtcttattt
     6841 aatgtaaata ttggtgctaa tttgaaatga cgctttattc atggggaacg cgtagcgcaa
     6901 atcaactaaa atcaatgtaa ccgtttgttg tttaaggttt aattgtctgt gtctgatatt
     6961 ttattgattg ttttaaatat tgtttttatt gtcattgcta taatattggt tatcatttgc
     7021 tgaatggatt cagtcttaat gggtgggttt ttaagggaca ggcatagagt aatgatacgt
     7081 atgcataacc aacatcttta ctcattatgt cattgaatgt tgacgctatg tgtttatgag
     7141 ggagaggtat tttcagttga tctggattgt taaattcata taatgcgcct ttgctcatga
     7201 atggatgcca gtacgtagtg ggaaattata aatattgaaa tagtccaact acttctttat
     7261 taccaaaaat gagtatctga attttaatat tgcattcttg cgtgattatc tcctgagttt
     7321 gtcttgtgat taccttttta aggtatttag cgtaactgtt tttgagcgag catcagaggt
     7381 aaagataatc tttttgatag tgatgtggga tgttatacgt atggcatcgc taatgtttat
     7441 ggttacccct tatgtgtgct caggaatcca caggtaatca ctcatactga acagcgataa
     7501 aagataaagg tgtgttcatg aattcatgtg attttcgtgt ttttctgcaa gagttcggta
     7561 caacggttca tttgtcattg cctggtagcg tatccgagaa agaacgactg ctactcaagc
     7621 tgctgatgca gggaatgtct gtaacagaaa tatcacagta cagaaatcgc agtgcaaaga
     7681 caatttcaca tcaaaagaaa cagctctttg agaaactggg gattcagagc gatattactt
     7741 tctggcgcga tattttcttt cagtacaatc cggagatcat atccgccacg ggtagtaata
     7801 gtcatagata tattaatgat aatcactatc accatatcgt cacgcctgaa gccatcagtc
     7861 tggcgttgga aaaccacgaa ttcaaaccgt ggatccaacc ggttttctgc gcgcagactg
     7921 gggtactgac gggctgtgag gtgcttgtcc gctgggaaca tccacaaacg ggaattatcc
     7981 caccggatca gtttattcct ctggcggagt catccggtct tattgtcata atgacccgcc
     8041 aactgatgaa acagactgcg gatattctga tgccggtaaa acatttgctg ccggataatt
     8101 tccatattgg tatcaacgtc tcggcgggtt gttttttggc cgcgggattt gaaaaagaat
     8161 gcctgaatct ggtcaagaaa ttaggaaacg ataaaatcaa actggttctc gagctaacgg
     8221 aacgtaaccc tattccggta acgccagaag ccagagcgat atttgacagc cttcatcagc
     8281 acaacattac ctttgcgctg gatgactttg gtacgggtta tgcgacctat cgttatctgc
     8341 aggcgtttcc ggtcgatttt attaagatcg ataagtcatt tgtgcaaatg gcgagtgtcg
     8401 acgaaatctc cggtcatatt gtggacaata ttgtcgaact ggcgcgtaag cctggtctga
     8461 gtatcgtagc ggaaggggta gagacccagg agcaggcgga tttaatgatc ggtaaaggcg
     8521 ttcacttttt gcagggctat ttgtactccc cgccagttcc gggtaataaa tttatctctg
     8581 aatgggtaat gaaagcaggt ggttgatgta aaccgctatt cacagcgcat cgggaggttg
     8641 gcagcgatta attctcccga tgcagtaaat cctgatagat ccccgttaat atgccatttg
     8701 gcgcaaattg ttttttaatc cactgcgtca cctgacccgt agcagaatgc tgagtcgcca
     8761 gcaacatgcg cgaatcctgg cgcggattat ggatttgtcg ggtaaccagc agggattgcg
     8821 ccatcgcctc acggaccatg taatccggca aaaaaccaat tccttcacct aaaatctggc
     8881 actgacattt ggtgttgaaa tctggcacca gaatcgactc ctgcccatgc agcaaccagc
     8941 ccactttttt attaatcgta tgcgcggtgt cctccaccat aatattaggg tagagacgca
     9001 gttggctttc ggcgatgggt tccggcacaa aggctaacgg gtgatccggg gcgatagcaa
     9061 aagcccagcg aatcgcgcca atttcagtgt aatcaatacc gccgccgtcc agtagtgtat
     9121 cgggtgcgcc gatggcgata ttggcctgat tattaataat cgcatcccag acgccgttat
     9181 acacttcggt ggtgacggta atctggcagg taggaaactg ctttttcagc acctgtaata
     9241 agcgcgcggt gtgtttgggc gtataaagca gctgattaat acaaatgcgc acacgcgctt
     9301 ctatgccctg cgaaatagta tctatgctac gtttgatggc ataaaaatca ttcagcaggt
     9361 cggtagcttt gcggaaaaaa taacgcccgg actccgttaa ctcaatgctg cgggtactgc
     9421 gagtgaacag caccacatcc agccccgtct ccatacgctt aatggtgtag ctaatggcgg
     9481 aagtggttaa acccaactct tctgcggctt tactgaagct gccaaaacgc gcagcggtag
     9541 taaatgccaa cagattctct tcggtaaaaa ttgagttcat taaaccattc tcgccattat
     9601 ctactatgaa tttctccagc tatgactgta gaggtatcgg ttaaagatag ataatcattt
     9661 ttgaataact tttaataccc gtcgcgttta gtcgtcgctt cgcttaattt ttgtccacca
     9721 tattctgaaa ttacccccac gcagttaccc gccagaaaac ccagtaacgt caccgtccag
     9781 ttcatcccgc tggcaaaaaa cagcgtcatg ccgagaaagc caccgggaat aaaggacagt
     9841 aaccagaaac gcccttgcca gaccactacc gccgagaagg gtacggtagt aattacgctc
     9901 gcccagaacg cggacagtcc acttacagag gccagccaac cagcagtcag cgcggcgaaa
     9961 aacgcccaca ccatcccgga ataattaacc gccagacttc tgataaaccc cgatttaccg
    10021 ccgcctgcgg cataaaaact gcaaaaggcg acgaagccaa tggttcctaa taattcccat
    10081 ccttgagaga gcccgacatg tgaagacact aactgccaaa gccctgcaca aataccgacg
    10141 gtgacacccg ttgccgtgag tccgttcatc ttgttcccct ctggttagac atggacgaaa
    10201 gctactctta catgtgcaga ctatagagaa ataaaacctg attgagtgaa agggtatcgg
    10261 gtcaaagaaa caaatattgc actaccgcac actgcgaaaa gattgttgaa tattattcaa
    10321 caaaaaggcg agatacggcg aattaaattt aagcgggttc ttttatcaga ttatcgttgc
    10381 tattctcaat tcatcgaaaa cagccacaaa aaccagggga atgtgaatga gtataaaaaa
    10441 tctacctgcc gactatttat tggctgcaca acagggtgat atcgataaag taaaaacctg
    10501 ccttgcgctg ggtgtcgata ttaatacctg cgatcgtcag gggaaaacgg caattacgct
    10561 ggcaagttta tatcagcaat atgcttgcgt tcaggcatta attgatgccg gagcggatat
    10621 taataagcaa gatcatacct gtttaaatcc ttttttaatt agctgtctga acgatgattt
    10681 aacgctacta cgaattattt taccggctaa acccgatctt aattgcgtaa cccgttttgg
    10741 cggtgtcggc ctgacgcctg cctgtgaaaa aggccattta agtattgtaa aagagctttt
    10801 ggcgcatacg gagattaacg ttaaccagac caaccatgta ggctggacgc cgctactgga
    10861 agcgattgtg cttaatgatg gtggtattaa acagcaggcg attgtgcagt tattgctgga
    10921 acacggtgcc agcccgcatc tgaccgataa atatggcaaa acgccactgg aactggcgcg
    10981 ggaacggggc tttgaagaga ttgcgcagtt actgattgcc gcaggtgcat aaaccgggag
    11041 gcttgctatc aacacaccag aaagacggtg tgtgtgggcg ctaactgcgg atgcggattt
    11101 tctggcgcag cgggggcaag gacaggttga acaggtcttt gccagagcgg taaatatcgc
    11161 actcccggct cgccagcagt tgctgacgct gctttgtgaa gagtacgaca atgcgccaaa
    11221 cagttgtcgg ttggcactca ctcactttga tgatctgttc cggcatggtg ataaggttca
    11281 gtttgacgat caaggtatta cggttggtca acatcttcat atagagatga gtcgttgtcg
    11341 gcgttggctg tccccaacct tgcaaatgac cgctgtgaat tttcacctta tcgcctggct
    11401 acagtggcac gacattattc atcagcacct gggggaaaat gaaaccctgt ttaattatcg
    11461 cggcgataat ccgttttatc aggcgttaaa taaagaatta catattaaac gacgggcagt
    11521 tattcaggcc gtaaacgata aacaaaatat cgcctcagcg gtcgccagta tgatggggtt
    11581 agggattggc cttacgccat cagccgacga ttatttaaca ggtctggcgc ttattttatt
    11641 tattcccggg catccggcgg aaaaatacaa agaggaattt tatctcggtc tgcaacgcgg
    11701 caaaaataat accacattat taagtgccat aacgctggaa gccgcattac aacaacgctg
    11761 ccgggaaaat attcatcgtt ttattcacaa cattatttat gacatccctg ggaacgcaac
    11821 tcaggcaata gaaaaaatta aacatattgg ctccagttcc ggctgcgaca tgctgtatgg
    11881 catggccgat ggttgtgcgc tgagccaaac ctacggaggg aattatgtca gttaaaatag
    11941 tcattaaacc gaatacctat tttgattctg tctcgctgat gtctatctcc acgcgtgcaa
    12001 ataaactcga cggcgtcgag caggcatttg tggcgatggc gaccgaaatg aataaaggcg
    12061 tgctgaagaa tttaggactg ctgacgccgg agctggagca ggcgaaaaac ggcgacctga
    12121 tgattgtcat caatggtaaa tcgggtgcgg acaacgagca gttgctggtg gagattgaag
    12181 aactgttcaa caccaaagcg caaagcggct cgcacgaggc gcgttacgcc actattggca
    12241 gcgccaaaaa gcatatcccg gaaagtaacc tggcggtgat ttcggtcaac ggtctgtttg
    12301 ccgctcgcga agcgcgtcag gcgctgcaaa acgatctcaa cgtgatgctg ttttccgata
    12361 acgtctcagt tgaagatgaa ctggcgctca agcaactggc ccacgaaaaa gggctgctga
    12421 tgatggggcc agactgtggc acggcgatta tcaacggcgc ggcgctctgt tttggtaacg
    12481 ccgtgcgtcg cggcaacatc ggtattgttg gcgcatccgg caccggcagt caggagttga
    12541 gcgtccgcat tcatgaattt ggcggcggcg tttcgcaact gattggcacc ggcgggcgcg
    12601 acctgagcga gaaaatcggc ggcctgatga tgctcgacgc catcgggatg ctggaaaacg
    12661 atccgcaaac tgaaatcatt gcgcttatct ccaaaccgcc tgcgcctgcg gtggcccgca
    12721 aagtgctgga acgtgcgcgc gcctgccgca agccggtggt cgtctgcttc ctcgatcgtg
    12781 gcgaaacgcc agtggatgag caggggctac agtttgcccg cggcaccaaa gaggcagcgc
    12841 taaaagcggt gatgctctcc ggcgtgaaac aggaaaatct cgacctgcat acgcttaacc
    12901 agccgttgat tgcggatgtg cgtgcgcgtc tgcaaccgca gcagaaatac attcgtggcc
    12961 tgttctgcgg cggcacgctg tgcgacgaaa ccatgttcgc ggtgatggaa aaacatggcg
    13021 atgtctacag caacattcag cccgatccgg aattccgcct gaaagatatc aaccgcagca
    13081 tcaaacacac cttcctcgac tttggcgatg acgacttcac caacggcaag ccgcacccga
    13141 tgattgaccc caccaaccgc atcagtcgct tgatcgaaga ggcgcgcgat ccagaagtgg
    13201 cggtgatcgt gatggatttt gtgctcggat ttggatcgca tgaagatccg gtcggctcca
    13261 ccatcgagac gatcaaagaa gcgaaagcga tcgccgctgc cgaaggacgc gagttgatca
    13321 ttctcgccta tgtgctgggt accgatcttg atacgccatc gttagaacaa caaagccaga
    13381 tgctgcttga tgccggagtg attctggcga gcagcagcac caataccgga ttgctggcgc
    13441 gtgaatttat ctgcaaaggg gaggaagcct gatgagccag tcactgttta gccaaccatt
    13501 gaacgttatt aacgtcggca tcgccatgtt tagcgatgac ctgaaaaagc agcatgtaga
    13561 agtgactcaa ctcgactgga cgccgccggg gcagggcaat atgcaggtgg tgcaggcgct
    13621 ggataacatt gccgattcgc cgctggcgga caaaatcgcc gccgctaacc agcaggcgct
    13681 ggagcgtatt atccagtcgc atccggtgct gattggtttt gatcaggcga ttaacgtggt
    13741 gccgggcatg acggcgaaaa ccattcttca cgccgggccg ccgatcacct gggaaaaaat
    13801 gtgcggcgcg atgaaaggcg cgctcaccgg agcgctggtg ttcgaaggac tggcgaaaga
    13861 tctcgacgag gcggctgaac tggcggcttc cggggagatc accttctcgc cgtgtcacga
    13921 gcacgactgc gtgggatcga tggcgggtgt tacctcggcc tcgatgttta tgcacatcgt
    13981 aaaaaacaaa acctacggca acatcgctta taccaacatg agcgagcaga tggcgaagat
    14041 tttgcgtatg ggcgctaacg accagagcgt gattgaccgc ctgaactgga tgcgtgatgt
    14101 gcagggacca atactgcgcg acgcgatgaa aattatcggc gaaatcgatc tgcgcttaat
    14161 gctggcgcag gcgctgcata tgggcgatga gtgccataac cgcaataacg ccgggacgac
    14221 gctgctgatt caggcgctga cgccggggat tattcaggcg ggttattccg tcgagcaaca
    14281 gcgcgaagtg tttgagtttg tcgccagcag cgactacttc tccggcccga cgtggatggc
    14341 gatgtgtaaa gcggcgatgg atgcggcgca tggcatcgaa tacagcccgt ggtcaccacc
    14401 atggcgcgta acggcgtcga gttcggcctg cgggtcagcg gcctgccggg gcaatggttt
    14461 accggcccgg cgcagcaggt gatcggcccg atgtttgccg gttataagcc ggaagattcg
    14521 gggctggata tcggcgacag cgccatcacc gaaacctacg gtattggcgg atttgctatg
    14581 gcgaccgcgc ccgctatcgt cgcactggtg ggcggcacgg tggaagaagc tattgatttc
    14641 tcccgtcaga tgcgcgaaat caccctcggt gaaaacccca acgtcaccat tccgctgctc
    14701 ggttttatgg gcgtgccgtc ggcaatcgac atcacccgcg tgggtagcag cggcattctg
    14761 ccggtgatca acaccgccat cgcccataaa gatgcgggcg tcggcatgat tggcgcgggc
    14821 attgtgcatc caccttttgc ctgcttcgag aaagccattc ttggctggtg cgaacgttac
    14881 ggcgtctgac ctacatgttc
//
LOCUS       DAOFQD010000114        14650 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.114, whole genome shotgun sequence.
ACCESSION   DAOFQD010000114 DAOFQD010000000
VERSION     DAOFQD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.114"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..369
                     /gene="puuP"
                     /locus_tag="QBF46_003644"
     CDS             <1..369
                     /gene="puuP"
                     /locus_tag="QBF46_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDQ6675777.1"
                     /translation="PALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFN
                     HFWRRKGMNKSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYL
                     IRRYRKVPLYDGDRTPVSET"
     gene            503..748
                     /locus_tag="QBF46_003645"
     CDS             503..748
                     /locus_tag="QBF46_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDQ6675778.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAECTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            1061..2704
                     /gene="sapA"
                     /locus_tag="QBF46_003646"
     CDS             1061..2704
                     /gene="sapA"
                     /locus_tag="QBF46_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDQ6675779.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            2701..3666
                     /gene="sapB"
                     /locus_tag="QBF46_003647"
     CDS             2701..3666
                     /gene="sapB"
                     /locus_tag="QBF46_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDQ6675780.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            3653..4543
                     /gene="sapC"
                     /locus_tag="QBF46_003648"
     CDS             3653..4543
                     /gene="sapC"
                     /locus_tag="QBF46_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDQ6675781.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            4543..5535
                     /gene="sapD"
                     /locus_tag="QBF46_003649"
     CDS             4543..5535
                     /gene="sapD"
                     /locus_tag="QBF46_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDQ6675782.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            5537..6343
                     /gene="sapF"
                     /locus_tag="QBF46_003650"
     CDS             5537..6343
                     /gene="sapF"
                     /locus_tag="QBF46_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDQ6675783.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            6411..6764
                     /locus_tag="QBF46_003651"
     CDS             6411..6764
                     /locus_tag="QBF46_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDQ6675784.1"
                     /translation="MIDKIKSNARDLRRNLTLQERKLWRYLRSRRFSDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            7132..7920
                     /gene="fabI"
                     /locus_tag="QBF46_003652"
     CDS             7132..7920
                     /gene="fabI"
                     /locus_tag="QBF46_003652"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDQ6675785.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            8064..9191
                     /locus_tag="QBF46_003653"
     CDS             8064..9191
                     /locus_tag="QBF46_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDQ6675786.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFTDESIRWRSSYEV
                     EKLPEDYTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSLNEYKKINTLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            9259..11193
                     /gene="rnb"
                     /locus_tag="QBF46_003654"
     CDS             9259..11193
                     /gene="rnb"
                     /locus_tag="QBF46_003654"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDQ6675787.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDEMVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(11270..11374)
                     /locus_tag="QBF46_003655"
     CDS             complement(11270..11374)
                     /locus_tag="QBF46_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675788.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            11428..13414
                     /gene="pdeR"
                     /locus_tag="QBF46_003656"
                     /pseudo
     CDS             11428..13414
                     /gene="pdeR"
                     /locus_tag="QBF46_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            13562..13735
                     /locus_tag="QBF46_003657"
     CDS             13562..13735
                     /locus_tag="QBF46_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675789.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            13825..14574
                     /locus_tag="QBF46_003658"
     CDS             13825..14574
                     /locus_tag="QBF46_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6675790.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSTLTVHVVDI"
BASE COUNT         3446 a         3697 c         3761 g         3746 t
ORIGIN      
        1 ccggcactca acgtcattat ggtcgggatt gtcgcgttgt cggcgctgtt cttcgattta
       61 gttaccgcca cagcgttaat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
      121 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
      181 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaacctc
      241 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttatgg
      301 tatctgatcc gccgctatcg caaagtgccg ttgtatgatg gtgacagaac gccggtgagt
      361 gaaacgtaat agtcggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
      421 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
      481 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
      541 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaatgca caccgctggc
      601 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
      661 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
      721 gtttttgaat caatggggta atgagtaatg atttaattgc agacgatcgg tcagtatcgt
      781 tgtccggata aagcgttcac gccgcatctg gctaacaatg acacgcactg cagcgtgctt
      841 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
      901 ttatagagaa atcacgggta cgaatgttga acagatggcg acgtcacctg ttcaacgacc
      961 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
     1021 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
     1081 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
     1141 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
     1201 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
     1261 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
     1321 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
     1381 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
     1441 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
     1501 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
     1561 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
     1621 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
     1681 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
     1741 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
     1801 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
     1861 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
     1921 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
     1981 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
     2041 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
     2101 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
     2161 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
     2221 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
     2281 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
     2341 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
     2401 cttccgtcct ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
     2461 cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcggcgcg
     2521 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattgccca ttttgccgct
     2581 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
     2641 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
     2701 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
     2761 ttcgttggct tcagcttgag ctatttcacc cctcatgcgc cgttacaagg cgcgtcactg
     2821 tggaatgcct gggtgttctg gtttaacggc ctgatccact gggattttgg tgtttccagc
     2881 attaacggtc aaccgattgc cgaacagtta aaagaggtct tcccggcgac gatggaactg
     2941 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
     3001 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
     3061 atcccggtgt tctggctggc gcttctgttg acgctgtttt gttcactcac gctaggttgg
     3121 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
     3181 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
     3241 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
     3301 ctgatgcgta tcagcactat cgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
     3361 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
     3421 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
     3481 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
     3541 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
     3601 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
     3661 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
     3721 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcggggctgg ctgtactgtg
     3781 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
     3841 attgccgccg tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
     3901 cggacgcgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
     3961 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
     4021 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
     4081 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
     4141 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
     4201 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
     4261 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
     4321 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
     4381 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
     4441 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
     4501 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
     4561 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
     4621 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
     4681 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
     4741 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
     4801 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
     4861 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
     4921 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
     4981 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
     5041 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgacc
     5101 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
     5161 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
     5221 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
     5281 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
     5341 agcgcgatgc cgcataaaag tcgcctcaat acgctacccg gcgctatccc actgctggaa
     5401 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
     5461 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
     5521 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
     5581 ccgctaccgg accggctggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
     5641 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
     5701 gctggcaaaa atgctggcgg gaatgataga gcctaccagc ggcgagttat tgattgacga
     5761 tcatccgctg cattttggcg attattcctt ccgcagtcag cgtattcgca tgatttttca
     5821 ggatccttcg acctcgttga atccccgtca acgtatctcg caaattctgg attttccatt
     5881 gcgtctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
     5941 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccgggca
     6001 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
     6061 tgaagccctc gcttcactgg atatgtcgat gcgttcgcag ttgattaatc tgatgctgga
     6121 attacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
     6181 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
     6241 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccggtcattt
     6301 tggcgaggca ttgacggcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
     6361 ccagattatt cacctcattc tgacaaaaga ttaaatcttg tcagaatgag gtgatagata
     6421 aaattaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
     6481 ggcgatatct tcgcagccga cgttttagtg atttcaaatt tcgccgtcaa catccagtgg
     6541 ggagctacat tctcgatttt gcttgctgct cggcgcgtgt agtcgttgag ctggatggtg
     6601 ggcagcatga tttagcagtt gcctatgatt ccaggcgcac tagctggctt gagtcgcagg
     6661 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag acggtgctgg
     6721 agaatattct gcaggaactg aaccgccggt caccctctcc ctgaaagagc gagggggcag
     6781 accgagccga atagctgttg tggtgaaaac atggagacgg tgctggagaa tattcggcaa
     6841 ggtctgaacc gtcccagcca tcgccatgaa agggttaggg gctgtatgag cctgtttgtt
     6901 gctggggtaa caatatttgc acaatacggt cccctcgccc ctctggggag agggttaggg
     6961 tgaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggc aactatagct
     7021 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
     7081 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
     7141 ctttccggta agcgcattct ggtaaccggt gttgccagca aactatccat cgcctacggt
     7201 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
     7261 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
     7321 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
     7381 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
     7441 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
     7501 ttcgttgcaa tggcaaaagc ttgccgctcc atgctaaatc cgggttctgc cctgctgacc
     7561 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
     7621 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
     7681 gttaacgcca tctctgctgg tccgatccgt actctggcgg cttccggtat caaagacttc
     7741 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtaccgt tactattgaa
     7801 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
     7861 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
     7921 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
     7981 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
     8041 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
     8101 atcatgaaac gcaatccagt actgcggagt atgacgttct gcctctggtc ccggaagccg
     8161 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagaaacg ctggccccct
     8221 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga attgttccct gaccagctta
     8281 ccgtgacccg ttcacagact ttcaccgctt atgaacgctt gtctacggcc ctgacggttg
     8341 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
     8401 ccgggcctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
     8461 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
     8521 tcggtcttac tgcctgggac tgtgtaatca ttaaccaaat cattggattt attggctttc
     8581 aggcgcggac cattgcgaca tttcaggctt atcttggaca tccggtacgc tggttacccg
     8641 gtctggagat acaaaactat gccgacgcgt cactgtttac tgatgaatca atacgctggc
     8701 gaagcagcta tgaggtggaa aaactacctg aagattacac aaaaagttca actgcagaac
     8761 tttgccaact ggctgaaaca ctctctctcc accctatttc actttccctt cttgaaaagt
     8821 tgttaaacag cacacgggtt aatacacagc cggataatca gcttgcggcg ttgttatgcg
     8881 cacggataaa tggcagccct gcttgttttg ccgcctgtat ggattcatta aatgaatata
     8941 aaaaaatcaa cacccttctg cgcaagggcg aaaatgaaat taaccaatgg gctgaccgtc
     9001 attctgttga gcacgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
     9061 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
     9121 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
     9181 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
     9241 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
     9301 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
     9361 ttcctggaag tcgacgcgca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
     9421 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
     9481 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaggttca gggcaaaaat
     9541 gaccgtctgg ccatcgttcc tgatcatcca ctcttaaaag acgccattcc ttgccgcgca
     9601 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
     9661 catccgctga aaggcgatcg ttctttctat gcagaactga cacaatacat cacttttggt
     9721 gacgatcact ttgtaccgtg gtgggttacc cttgcacgcc ataatctgga aaaagaagca
     9781 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgaccgcg
     9841 ctggattttg tcaccatcga cagtgccagc acagaagata tggatgacgc ccttttcgct
     9901 aaggcgttgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
     9961 attgctgaag gcagtaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    10021 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    10081 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    10141 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    10201 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    10261 gaacaagtcc gtttgttagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    10321 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    10381 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    10441 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    10501 gtgcatatgg gctttgatcc ggcaaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    10561 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    10621 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    10681 gctgaaatta gcactgaacc cggtcctcac tttggcctcg ggctggaagc atacgccacc
    10741 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    10801 atcaaaggcg aaactgcgac gcgtccacag gatgaaatca ctgtccaaat ggccgagcgt
    10861 cgccgtctca atcggatggc agaacgtgat gttggtgact ggttatacgc acgcttcctg
    10921 aaagacaaag ccgggaccga cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    10981 ggcatgcgtg ttcgtttggt tgataacggc gctatcgcct ttattcctgc acctttctta
    11041 cacgctgtgc gcgatgaaat ggtttgcagc caggaaaacg gcaccgtaca aattaaaggt
    11101 gaaacggttt ataaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    11161 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttccttatga
    11221 atgggccgtt tatttccccg ctctaccttc atcatatccc ggcagttttt taactgactt
    11281 tcgtttgaaa actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaaacaa
    11341 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    11401 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    11461 aactttctcg gatcgcacaa ttcatactgg cggttgacgg aaagcagcga tgttttgcgc
    11521 ttttctacca ccgaaaccac agaacctgaa cgtatgttgc agttatctgc cgaacaggct
    11581 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    11641 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    11701 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    11761 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tcattctcga cagccggggg
    11821 gaatattcaa cgcttcaatc gcttatgtga agattacaca gggttgaaag aacacgacgt
    11881 cattgggcaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    11941 caataaccgt gtattttttc gaagcggcaa tgcgtatgaa gtcgaactgt ggataccaac
    12001 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    12061 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcgctcagga
    12121 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    12181 gcaggagtta atcgatcacg ctattaatca ggcaaataac aataaagttg gggttgtgta
    12241 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    12301 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    12361 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    12421 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    12481 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    12541 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    12601 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    12661 caacttgcgt aaagcactgg aaaacgatca gttggttatt cactatcaac cgaaaatcac
    12721 ctggcgtggc gaagtgcgca gtctggaagc actagtacgt tggcagtcac ctgaacgtgg
    12781 gttgattcca ccgttggact tcatttccta cgccgaagag tcagggctaa ttgtgccttt
    12841 aggccgttgg gtgattctcg atgtcgtacg ccaggtggcg aagtggcggg ataaaggcat
    12901 taacctgcga gtggcggtaa acatttctgc acgtcagctc gccgatcaaa ccattttcac
    12961 cgccctgaaa caggttctcc aggaactgaa ttttgaatac tgcccaatag atgttgaact
    13021 gacagagagt tgtctgattg agaatgatga actggcactg tctgttattc aacaatttag
    13081 ccaactaggt gcgcaagtgc atctggacga ttttggtacc ggctactctt ctctttcgca
    13141 actggcacgc tttccgatcg atgccatcaa acttgaccag ggttttgttc gagatattca
    13201 caaacagcct gtctcgcagt cactggtccg ggcgatcgtc gctgtggccc aggcattgaa
    13261 tcttcaggtg atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa
    13321 cgggatcaat gagcggcaag gatttttgtt tgccaaaccg atgcccgccg tcgccttcga
    13381 acgttggtat aaacgctatc tgaagcgcgc ataagtagat gaatatatat acttagcgtt
    13441 atattccgtc attatatatc gtattccagg atgataatta gccgttgctg ttaaacactt
    13501 ctcttttagc ggcaattttt gcattattca ttttccccct tttgctataa ggatgtcctt
    13561 gatgtctgaa tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct
    13621 tgtggcaggc gattaccact ctgcgatcca taatctcgag atacttaaag cagaactctt
    13681 gcgccaggtt gctgaatcta cgccagacat cccgaaggca ccgtgggaga tttaacaatc
    13741 gtgccttcag gttttcctat ttgaggatgc ccggcgcgtc agtatttttt tatttagtat
    13801 tataacgtta taagaattac agcgatgaat tcccgacaac aaactattct acagatggtc
    13861 attgaccagg gtcaggttag cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt
    13921 accattcgcc aggatctcaa caccctcgaa aaactgagtt acctccgccg tgcacatggc
    13981 tttgcagttt cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg
    14041 aagcgtgaac tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac catctttatc
    14101 gaaaatggta gcagcaatgc cctgctcgcc cggactctgg gcgaacagaa gaaaaatgtc
    14161 actatcatca cagtcagcag ctacattgcg catttgctga aagacgcgcc ttgtgaagtc
    14221 attttgctcg gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc tttgacacgc
    14281 cagtgcatcc agcaggtaca tttcagcaaa gcatttattg gtattgatgg ctggcaacct
    14341 gaaactggat tcaccggtcg cgatatgatg cgtaccgatg tggtcaatgc cgtgctggaa
    14401 aaagagtgcg aagcgatagt cctgactgac agctcgaaat ttggtgctgt acattcatac
    14461 tccatcggtc ccgttgagcg attcaatcgc gtgattaccg attcgaaaat acgcgccagc
    14521 gatctgatgc atcttgagca cagtacactc accgttcacg tcgttgacat ttaaccattt
    14581 cccttctcta cggatgattt gcagtttggc aaatcatccg ctctaagatg attcctggtt
    14641 gataattaag
//
LOCUS       DAOFQD010000115        14481 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.115, whole genome shotgun sequence.
ACCESSION   DAOFQD010000115 DAOFQD010000000
VERSION     DAOFQD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.115"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..414)
                     /gene="exuT"
                     /locus_tag="QBF46_003659"
     CDS             complement(<1..414)
                     /gene="exuT"
                     /locus_tag="QBF46_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDQ6675791.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGY"
     gene            897..2309
                     /gene="uxaC"
                     /locus_tag="QBF46_003660"
     CDS             897..2309
                     /gene="uxaC"
                     /locus_tag="QBF46_003660"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDQ6675792.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            2324..3811
                     /gene="uxaA"
                     /locus_tag="QBF46_003661"
     CDS             2324..3811
                     /gene="uxaA"
                     /locus_tag="QBF46_003661"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDQ6675793.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            3894..4445
                     /locus_tag="QBF46_003662"
     CDS             3894..4445
                     /locus_tag="QBF46_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDQ6675794.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(4450..5694)
                     /gene="sstT"
                     /locus_tag="QBF46_003663"
     CDS             complement(4450..5694)
                     /gene="sstT"
                     /locus_tag="QBF46_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDQ6675795.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANRALRN"
     gene            complement(6310..7275)
                     /gene="alx"
                     /locus_tag="QBF46_003664"
     CDS             complement(6310..7275)
                     /gene="alx"
                     /locus_tag="QBF46_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDQ6675796.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(7558..8544)
                     /locus_tag="QBF46_003665"
     CDS             complement(7558..8544)
                     /locus_tag="QBF46_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDQ6675797.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(8623..9306)
                     /locus_tag="QBF46_003666"
     CDS             complement(8623..9306)
                     /locus_tag="QBF46_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDQ6675798.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWNDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPPKE"
     gene            complement(9383..9886)
                     /locus_tag="QBF46_003667"
     CDS             complement(9383..9886)
                     /locus_tag="QBF46_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDQ6675799.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9972..11108
                     /gene="rlmG"
                     /locus_tag="QBF46_003668"
     CDS             9972..11108
                     /gene="rlmG"
                     /locus_tag="QBF46_003668"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDQ6675800.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARQINCTFNEPQLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            11393..11707
                     /gene="higB"
                     /locus_tag="QBF46_003669"
     CDS             11393..11707
                     /gene="higB"
                     /locus_tag="QBF46_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HDQ6675801.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            11704..12120
                     /gene="higA"
                     /locus_tag="QBF46_003670"
     CDS             11704..12120
                     /gene="higA"
                     /locus_tag="QBF46_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDQ6675802.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(12165..14183)
                     /gene="fadH"
                     /locus_tag="QBF46_003671"
     CDS             complement(12165..14183)
                     /gene="fadH"
                     /locus_tag="QBF46_003671"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDQ6675803.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSAFQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVAEDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPTGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT         3594 a         3831 c         3772 g         3284 t
ORIGIN      
        1 gtaacctacc gcgatggaac gctctttcgc cggaaaccat tcggagctgg ctttcagacc
       61 cgccgggatc atcgctgctt ccgcggcacc gactgcacca cgagcaatcg ccaggccacc
      121 ccagctacca gccagcgcgg ttgcaccaca gaatacggcc cacagcacag caaacattgc
      181 ataaccgatt ttcgtaccca gcacatccag tacatagccc gctaccggtt gcatgaccgt
      241 ataagcagca gaataggctg cgatgatata ggaatactgt tgggtggaga tgtttaactc
      301 ttccatcaga gttggcgcag ctgccgccac agtgttacgc gtcaggtaac caagcacggt
      361 gccaagcgtc accagtgcga tcatatacca acgtaaccct ttaattttac gcatgtaaaa
      421 cctcatcgtt ttgttatatc cgcaggaagc gcggccatca tcccgccaga ggctgaagat
      481 gttagttacg accgggtaac cgaaaaaaaa ccggcacgcc ccgaacgttg ccatgaaagt
      541 ttcgtgctaa ttcggcttcc gtaccggtta acccgattca tctaacaaat gaatgaatcg
      601 gcaatttgca ttccgtcgac ttatgatttg cgacggcaga aagataactt gtcatacaac
      661 tttaaaaggt gagagccatc acaaatgtgc gaatatttgt agggacatta cctgacgaca
      721 gcgaggccag tactggcgcg gcctgcagag agatttacca ctttgagagt aatcttttta
      781 actacgttta ttgatctaac tcacgaaaat atcttcggac tctggaaatt ggtgtgataa
      841 ctttgtcagc atcgcaccat aagcaagcta gctcactcgt tgagaggaag acgaaaatga
      901 ctccgtttat gactgaagat ttcctgttag ataccgaatt tgcccgccgt ctgtatcacg
      961 actacgcaaa agaccagccg attttcgatt accattgcca tttgccgccg cagcagattg
     1021 cggaagacta tcgttttaaa aacctgtatg acatctggct gaaaggcgat cactacaaat
     1081 ggcgcgctat gcgtaccaac ggtgtggccg agcgtctgtg taccggtgat gcgtctgacc
     1141 gtgaaaaatt tgacgcctgg gcggcgactg ttccgcacac tatcggcaac ccgttatacc
     1201 actggacgca cctcgaactg cgccgtccgt ttggtatcac tggcaaattg ctttctccgt
     1261 caactgccga tgaaatctgg aacgaatgta acgaactgct ggcgcaggat aacttctccg
     1321 cgcgcggcat catgcagcag atgaacgtga aaatggtcgg caccaccgat gacccgatcg
     1381 attctctgga gcatcacgca gagatcgcca aagacggctc tttcaccatc aaagtgctgc
     1441 cgagctggcg tccggacaaa gccttcaaca tcgaacaggc gacctttaac gactacatgg
     1501 cgaagctggg cgaagtttcc gataccgaca ttcgccgctt tgctgacctg caaactgccc
     1561 tgaccaaacg tctggatcac ttcgccgctc acggctgtaa agtgtctgac cacgcgctgg
     1621 atgtggtgat gtttgctgaa gcgaacgaag cggaactgga cagcattctg gcgcgccgtc
     1681 tggctggcga aaccctgagc gagcacgaag tggcgcagtt caaaactgcg gtactggtgt
     1741 tccttggtgc cgaatatgca cgtcgcggct gggtacagca gtaccacatt ggcgcgctgc
     1801 gtaataacaa cctgcgtcag ttcaaactgc tggggccgga tgtaggcttt gactccatca
     1861 acgaccgtcc gatggcggaa gagctgtcta agttgctgag caagcagaat gaagaaaacc
     1921 tgctgccgaa aaccattctc tactgcctga acccgcgcga taacgaagtg ctgggcacca
     1981 tgatcggtaa cttccagggc gaaggtatgc cgggcaaaat gcagttcggt tccggctggt
     2041 ggtttaacga tcagaaagac ggtatggaac gtcagatgac ccaactggcg cagctcggtt
     2101 tgctgagccg ctttgtcggt atgctaactg acagccgtag cttcctgtca tacacccgtc
     2161 acgaatactt ccgccgcatt ctgtgccaga tgatcggtcg ctgggtggaa gcgggcgaag
     2221 caccggcgga catcaacctg ctgggcgaga tggtgaaaaa tatttgcttt aacaatgcgc
     2281 gtgactactt cgccattgaa ctgaactaag gtctggggtt gatatgcaat acatcaagat
     2341 ccatgcgctg gataacgtcg cggtcgcttt agcggatttg gctgaaggca cagaagtcag
     2401 tgtcgataac caaactgtta ggctgcgcca ggatgttgct cgtggacata aatttgcgtt
     2461 aacgaatatc gcaaaagggg ccaacgtcat caaatatggc ctgccgattg gttatgcatt
     2521 ggcggatatt gcggcggggg aacacgttca cgcccacaat acgcgcacga atctgagcga
     2581 tctggatcag tatcgctatc aacctgattt tcaggatctg cctgcgcaag cggcagatcg
     2641 tgaagtgcag atctatcgtc gcgctaacgg cgatgtcgga gtgcgtaatg agctgtggat
     2701 cctgccaacc gtgggctgtg tcaacggcat cgcgcggcag atccaaaatc gtttcctgaa
     2761 agagaccaac aacgccgaag gtactgacgg cgtgttcctc ttcagccaca cctacggctg
     2821 ctcacagctg ggcgacgatc acattaatac ccgcaccatg ctgcaaaaca tggtgcgcca
     2881 cccgaacgca ggcgcagtgc tggtgattgg tctgggctgt gaaaacaacc aggttgccgc
     2941 attccgtgaa acgctgggcg atatcgatcc tgaacgcgtt catttcatga tctgccaaca
     3001 gcaggatgat gagatcgaag ccggaatcga gcatttgcat cagctgtata acgtgatgcg
     3061 caacgataaa cgcgagccag gcaaactcag cgaactgaag tttggtctgg agtgcggtgg
     3121 ttctgacggt ctttctggta ttactgctaa cccgatgctg gggcgtttct ctgactacgt
     3181 gattgctaac ggcggtacta ccgtactgac cgaagtgccg gagatgtttg gcgcagagca
     3241 gttgctgatg gaccattgcc gcgacgaagc aacgtttgaa aaactggtca ccatggtcaa
     3301 cgacttcaaa cagtacttta ttgcccatga tcagccgatc tatgaaaacc catcgccggg
     3361 gaacaaagcg ggcggtatca ccacgctgga agacaaatca cttggctgta cccagaaagc
     3421 gggttccagc gtcgtggttg acgtgctgcg ttacggcgag cgtctgaaaa cgccagggct
     3481 gaacttgtta agtgcgccgg gtaacgatgc cgtagcgacc agcgccctgg cgggtgcggg
     3541 ctgccatatg gtgctgttca gtactggtcg tggcacgccg tatggtggat ttgtgccgac
     3601 ggtgaaaatc gccaccaaca gtgaactggc ggcgaagaaa aaacactgga tcgactttga
     3661 cgcgggtcag ttgatccacg gtaaagcgat gccgcagttg ctggaagaat ttatcgacac
     3721 catcgttgag tttgccaacg gtaagcaaac ctgtaacgag cgtaacgact tccgcgaact
     3781 ggcgatcttc aaaagcggcg taacgctata aagtcgaaat gagttgttaa taaaaaacgg
     3841 cgtttcataa tgtgaagcgc cgtttgcttt tccatacctg ctaaggattt atcatgaccg
     3901 cgtattggct ggcccagggc gtgggtgtca tcgcctttct gattggtatc acaacatttt
     3961 tcaatcgtga cgaacgtcgc ttcaaaaagc agctttcggt ctatagcgcc gttattggcg
     4021 tacatttttt tcttctgggc acctatcccg ctggtgccag tgccatcctt aatgccattc
     4081 gtacattgat taccttacgc acgcgcagct tatgggtaat ggcgattttt attgtgctga
     4141 ctggcggaat tggcctcgcg aagttccatc atcctgtcga actattgccg gttatcggca
     4201 cgattgtcag tacctgggcg ctattccgct gtaaagggct gaccatgcgc tgcgtaatgt
     4261 ggttttcaac gtgttgctgg gtgattcaca acttctgggc ggggtcgata ggcggcacga
     4321 tgattgaggg tagttttctg cttatgaatg gcctgaatat cattcgtttc tggcggatgc
     4381 agaaaagggg aattgatccg tttaaagtag agaaaacccc tcccgccgta gacgaaaggg
     4441 gttaaacaat taattacgca gggctctatt tgccagacga tcgtcttccg cctggcaagc
     4501 tgccgcagtg aacagcacgt cagttgaaga gttcagcgcg gtttcgcaag agtcctgcaa
     4561 tacgccgatg ataaagccga cggcaaccac ctgcatggcg atatcgttcg aaataccgaa
     4621 catattacag gccagcggga tcagcagcag agaacccccc gccacgccgg atgcgccaca
     4681 ggcacacaga gaagccacca cgctcaacag cagcgccgtg ggcagatcga ccgggatacc
     4741 cagcgtatta accgcagcca gcgtcaacac ggtaatggtg attgctgcgc ccgccatatt
     4801 gatggtggca cccagcggaa tagaaacgga ataggtatcg cgatccagat tcagcttttc
     4861 acacagtgcc atattcaccg gaatgttcgc cgcagagctg cgggtgaaga aggcatacac
     4921 gccgctttcg cgcaggcaca gcagcaccag cgggaacggg ttacgacgaa ttttccacca
     4981 caccagcaat gggttaacca ccagcgccac cagtaacata cagccaacca gcacgaccag
     5041 cagttgcgcg tagccccaca gtgtggagaa accggtggtt gccagggtag aagaaaccag
     5101 accaaaaata ccaatcggtg cgaagcgaat gaccagtttc accataaagg tgacggcatt
     5161 cgacatatcg ttgaccaggt ttttggtggt ctcgttaccg tgacgcagtg cgaagccgag
     5221 gccgatcgcc cacaccagaa tcccgatgta gttacctttc agcagcgcgt cgatggggtt
     5281 ggaaaccatg ctcattacca gcccgcgcat cacttcgaca atgcctgacg gcggcgaaat
     5341 atcacccgcg ctactggata aatgcagggt agaagggaag gcaaagctga agactactgc
     5401 ggccagagca gcagagaagg tgcccagtag atagaggaac aaaatagggc ggatattggt
     5461 tttctgaccg tgctggtggt tagcaataga tgccattacc agcatcaaca ccaggatggg
     5521 ggcaacagct ttcagtgcgc cgacgaacaa agtacctaac agaccaacag cttccgccgc
     5581 gggttttgag atccatgcca gaagaatccc cagaacaagg ccgaccagga tttgttttac
     5641 caggctgcca tgagccagac gccggaatag ccccggtgaa cgttgcgtag tcatttttcg
     5701 atcctttcat tgtgttatcg cacatccctg gtgcgttctg tcgacgcatc tttcaggatg
     5761 taacaaagtg tttgcaaggt cgagtataag gaaagttaca gcttcgggaa gcgaaaaatg
     5821 ctggatttta aagagaggtc atgttttgca acttaattgt tacatttgtt tgacataaac
     5881 agagggaagg actggctact aaggctgatt agataacata tgggcaaaac tatttggaat
     5941 atgagaacgg ttgcgctcct ttaatgtgct atgccatata aagtcactgg cgcagggagc
     6001 gcgcaggggg cggccaatcg ccgccgcccc ctgcaccccc aggctctggc gaacaaaatc
     6061 gccgctgcgc gattccctca gcttatccct tgcggctacc gggtcgggcg caagggaacg
     6121 tccctgtaag atgcgccctc gccgcgcatc catgcgcgtc gccccggcct ccaaggaacg
     6181 cttcggcgat tttgacgcca ccaacacccg tgcggtcttt ttgtaaagag ctacaagccg
     6241 attacataat taatagctca cctttgtctg gaatgagtta tctctaggaa tggcttaata
     6301 tacagcgaat cattctcccc gctgcttatc atgccgataa ttcacccagg cgttgataat
     6361 aaacgtcatc accagaatgc caaacaccac gcccagcgag acggcgattg gaatatggta
     6421 gaagtcgaca atcagcatct tgataccgat aaacaccagg atcaccgcca ggccatattt
     6481 gagcatcgag aaacgctctg ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag
     6541 gatcgcaaac aggtttgagg tcagcacaat gaacgggtca gtggtcacgg cgaagatagc
     6601 cggaatgcta tccacggcaa aaatcacgtc gctcaattcc accagaatca gtaccagcat
     6661 cagcggtgtg gcgtacagca atccattctt acgcacaaag aaatgctcgt tgtcgatggt
     6721 gtcggtcatg cgcaaatgac cgcgtagcca gcgcaccagc ggcttgtcgc caatgcctga
     6781 ttcatcttca tgggcgagcg ccatcttcac gccggtaaac agcaggaagg caccgaagat
     6841 atacaggatc cagtcgaact gcgaaatcag ccagctgcct gtgaagatca tgatggtacg
     6901 cagaacaatc gccccgagca cgccatacac cagcacgcga cgttgtaatg ccgccggaac
     6961 ggagaaatag ctgaacaaca tcagccagac aaagacgtta tcgaccgcca gcgatttctc
     7021 aatcagataa cccgtgagaa aggccagtgc ctgtggatcg gcaacggcgc gaccttcggt
     7081 ttgcaccaga taccaccaga aagcggcgtt aaacagtaac gacagcgtca cccagaccag
     7141 cgaccaggcc gcagcctgtt tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa
     7201 caggtcgata gccagcataa tggcgacaac cacagcgaat ccgccccata gcaacggcgt
     7261 gccgacagta ttcatagaag ttccttacac ataaaaaaaa cggccaacgt cggaagacgt
     7321 cagccgctgc tttttatgca tagacctcgc cttccggcaa ggtctcactt acaacaaaaa
     7381 aggaatacgt ctgcgtattc ctttcgggtt gcccggtgac cggatgtggt tttcacacat
     7441 cgtaatgacg atcgaccggc aatgaagtta ctcccctttg caggtaacaa agtatgacag
     7501 accattcagc cagtcaatgg tctgttacat accttttaca ctgttttact ttggcaatta
     7561 tagttttacg ctatctgccg gaaaaatcac cccagtctgg cggcggatct cggtcagcag
     7621 tttggctgtg atgcgactga ccgccagccc tggatgatcc accagatgct catccaccag
     7681 ggtagcgaac agctctgctt cgtagagcat ggtattaata tgctgcggct gggtgagatc
     7741 ctgcatctgg ctgccacgcg gcacgaagca cactttctgg cattcagaca gtttttcaat
     7801 caccagcgat cccgcttcgc cctgaatctc gcttgccagg acagaatcac tgacctttga
     7861 gtgctgcaag gtgacgctga aatcaccgta atccatcacc accacgccgt gggcatctac
     7921 accacttgcc agtaaactgg cggttgcctg cacgcttttc ggctcaccaa ataacgctac
     7981 cgccgacgcc aggcagtaaa agccgatatc cataattgaa ccgttagaga atgccggatt
     8041 aaaggtgttg ggattctcac catccaggta acgttgatac cgcgaggaat actggcaata
     8101 gttgaaaaag actttacgca atttgccgac tttcggcagc gcctggcgca gcaaatgaaa
     8161 gttcggcagg caggcggttt taaatgcctc aaacagcacc acctgatttt cccgcgcaca
     8221 ggcaatggcg gcatccactt ccgccagatt cgacgccagt ggtttctcgc aaatcacatg
     8281 aattttatgg ctaaggaaaa gttgtgtctg ggaaaagtgc agggcattcg ggctggcaat
     8341 atacaccgcg tcaatggcat cgctttccgc catcgcttcc agcgaggtaa acagatgctc
     8401 gacagaaaaa tcattggcga agtgctgggc ctgttcaagg ctgcgggaat atacggcggt
     8461 taacttgtat ttaccgctct catgggcggc ctcgacgaac tggcgagtga tccagttcgt
     8521 accaatcaca gcgaaacgta tcataacgcg tggatactcc ataaagggat tctgccgtca
     8581 atgtagcacg cctttttcat cttcgacgtg cgcagatacg cattattctt tcggcggaca
     8641 gccatgctca ctaaacggcc caatcatgac cgatggcccg agatatttcg gctgacgatg
     8701 gaggattttt gcgtcaatca ccgcgctgac gcgagcgagt ttctcccgca gttgagtata
     8761 aaaaccgtgc ttcatattca ggtctggcgc gttaaacccg atagcgtcga tgccgtactg
     8821 ttttgccagc cagatggcgc gttggttatg aaattcttgc gagataatgg tgatatggtt
     8881 ttccccaaac acctttttgg cacgcaccac tgaatcaagc gttgagaatc cggcatagtc
     8941 gcagaagatc actttcgccg gtacaccttt agcgatcaaa gcctgctgca tacctgatgc
     9001 ttcatcgtaa tttttacgtc cgttatcgcc actcaccagt agccatttca cttttccggc
     9061 gtgatacaac gctgctgcgg tatcaatacg tcgggtaaag tagcgattac ccggcctcgc
     9121 ccccagcaat aaacccacgt tacgcgccgg aacggcgttg acatcgttcc aggtcagctg
     9181 cttgctagcg ttgaccataa cgcgatcggc aataaaaatg gtcgctcctg ctataagcaa
     9241 caataaacag ccaatcttaa gagtccggcg agaaaaacaa atacggagaa gaaggcgggg
     9301 ctgcatggtc gttatcctta acgaaagggg ggaggtcgat tttaggcgct aaagaaagaa
     9361 aagataacgc ctgacatcat tttcagattt tgtcctgccc aagcgacaac tgggttagcc
     9421 acagtcgggt gtcgaactca agctggtggt actgcggctc catatggcag cacaattgat
     9481 agaacgcttt gttatgctct ttctccttca ggtgtgccag ctcgtgcacc acgatcatgc
     9541 gcaaaaacgg ttccggcgcg ttgcgaaaca cggtagcgac gcggatctct gcttttgctt
     9601 tcagcttgcc gccctgcaca cgcgaaacgg cagtatgtaa cccgagcgcg ttttttagta
     9661 catggatctt attgtcatac atcactttat tgatcggcgg ggcattgcgc agaaactgat
     9721 ttttaagatc ctgagtatat tgccagaggg ctttatcggt ggcgtagtcg tgtgttcccg
     9781 gataacgttt tgccagcaca tcgcccagac gctgttcgtt aatcagcgtt cgcacctggg
     9841 aaagtagctg ctcgggataa ccctgaagat aagtaagatt gctcataacc gcccacgaaa
     9901 aagagaaaaa gggtatactc acgcaccctt ttccaggggc aacgtcgaaa ttttatcatt
     9961 caggagggcc gatgagccac ttagacaacg gtttccgttc actgacacta caacgttttc
    10021 cggcgacgga tgacgttaac ccactacagg cgtgggaagc ggcggatgaa tatttgctgc
    10081 aacagttgga cgacacagaa atccgcggcc cggtgttgat cctgaatgat gcctttggtg
    10141 cgttaagttg tgcgctggcg gaacataagc catacagcat tggcgactca tacatcagcg
    10201 aactggcgac gcgcgagaat ttacgcctca acgggattga tgagtcgagc gtgaagtttc
    10261 tcgacagcac cgccgactac ccgcaacagc cgggggtggt gctgatcaaa gtgccgaaaa
    10321 cactggcatt gctggaacaa caactgcgtg cgctgcgcaa agtggtcacg tcggatacac
    10381 gtattattgc cggtgccaaa gcccgtgaca ttcacacttc cacgctggaa ctgttcgaaa
    10441 aagtgctcgg cccaaccacc accacgctgg catggaaaaa agcgcgccag attaattgca
    10501 ctttcaatga accgcagctg gccgatgcgc cgcagaccgt tagctggaag ctggaaggta
    10561 ctgactggac tatccacaac catgcgaatg tcttctcccg caccgggctg gatattggcg
    10621 cacgcttctt tatgcaacat ctgccagaga atctcgaagg tgagattgtc gatctcggct
    10681 gtggtaacgg cgttattggc ctgacgctgc ttgataaaaa cccgcaggcg aaagtggtgt
    10741 ttgtcgatga atcaccgatg gcggttgctt ccagccgttt gaacgttgaa accaacatgc
    10801 cagaggcgtt ggatcgctgc gagtttatga tcaacaacgc gctctccgga gtggagcctt
    10861 tccgctttaa tgctgtgctc tgcaacccgc cgtttcatca gcaacatgcg ctgaccgata
    10921 acgtcgcctg ggagatgttc caccacgccc gccgctgcct gaaaatcaac ggcgaactgt
    10981 atatcgttgc caaccgtcac ctggattact tccataaact gaagaagatt ttcggcaact
    11041 gcaccaccat cgccacgaat aataaatttg tggtgctgaa aacagtgaag ctggggcgtc
    11101 gtcggtaaga ttgggagtta cctgccggat gcggcgttaa cgccttatcc ggcctacgtt
    11161 cagcacgtta ctcccaggcc ggataagacg cggcaagcgt cgcatccggc aatgtctgcc
    11221 cagttctgga atcgcgcttc cataaaacaa aaaatccctc tcaccatcct ctacattctc
    11281 ttgtttagcg tttttctacg tttattcttc cgtcacacag ataaattcat ccgttgccaa
    11341 tctgtcaacg gatgttatca tattgccaat ttgtcaacgg acgtgatgaa gaatgcacct
    11401 gataactcaa aaagcattga aagatgctgc ggaaaaatac ccgcaacata aaacggagtt
    11461 ggtggctctg gggaacacga ttgctaaggg atatttcaaa aaacctgagt cattaaaagc
    11521 agtattccca tctctggata acttcaaata tctggataag cattatgttt tcaatgttgg
    11581 gggcaatgaa ttacgtgttg tagcaatggt cttttttgaa tcgcaaaagt gctacatacg
    11641 tgaagttatg acgcataaag aatacgattt ctttaccgct gttcatcgta ctaaggggaa
    11701 aaaatgattg cgattgccga catcttgcaa gcaggagaaa agctaactgc ggtggcacct
    11761 tttctggcgg gtattcagaa cgaggaacaa tacacccagg cgctggaact ggtagatcat
    11821 ctgctgctca acgatcctga aaaccccttg ctggatctgg tgtgtgcaaa aataaccgcg
    11881 tgggaagaat cagcgcccga atttgcggaa tttaatgcca tggctcaagc catgcctggc
    11941 ggtatagccg tgattcgtac ccttatggat caatatggtt taaccctttc cgatctgccg
    12001 gaaattggca gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa attaacgctg
    12061 gaacacgcta aaaaattggc aacgcgattc ggcatttctc ccgccttgtt tattgattaa
    12121 cgcgacgtgc cggatgcgtt gcatccggcg cttaaaataa cgatttaaat ctccagcgcc
    12181 agccgtgttc cctgggcaat tgcccgtcgt gcgtccagct ccatagccac atcgcagccg
    12241 ccaattaaat gcacagtttt cccgctatca atcagcggtt gcgccagcgc gcggtttggc
    12301 tcttgccctg cgcagatcac cacattgtcc actgctaata cctgcgtttc gccgttgatc
    12361 accacatgca gcccgtcatc gtcaatcttc tgataactta cgcctgggat cattttcaca
    12421 ccccgcgaga gcagggtggt gcgatggatc cagccggtgg ttttgcctaa cccctgtccc
    12481 ggtttgctgg ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg gatctgcatt
    12541 ccctgcgggc ttaagccacc agcctgttgt aggctgctgt cgatccccca ctcattacag
    12601 aacccggcga tattctggct ggtggattcg cccggctgac ttaaatacat cgccgtatca
    12661 aaaccaatcc cgccacaacc gatgatggca accttgttgc ctaccggcgc tttgtcgcgc
    12721 agtacatcga gataactcaa taccttcgga tgatcgatcc cgtcgatagg cggaacgcgc
    12781 ggcacgatcc cactggcgag gatcgtttca tcgaacgcct gtaactgatc cgccgtcacg
    12841 gtgtgattga gttttagcgt cacgcccgtt acttcgatca tccggcggta gtagcgcagc
    12901 gtttcgtaaa actcctcttt gccggggatc tgtttggcaa tattaaactg cccgccgatc
    12961 tcgctgtgcg catcaaacaa tgttacctga tgcccacgcg ccgccgcgtt aatggcaaac
    13021 gccagcccag tcggtcccgc accgaccacc gccagatttt ttttctgcac ggcgggaagg
    13081 attggcattt tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt gactttgcca
    13141 acgaagatct gatcgaggca ggcctgattg cagccaatgc aggtgttgat ctcatcggct
    13201 cgccctgatt gcgcttttga cagcagttcc gcatcagcga gaaatggtcg cgccatcgat
    13261 accatatcgg catcgccgcg cgagagaata tcctcggcaa cctgcggatc gttaatccgg
    13321 ttggtggtca ccagcggcag cgagacgtga cctttcagtt tgcgcgtgac ccagctaaat
    13381 gcaccgcgcg gcacgggcgt ggcgatggtc ggaatacgcg cttcatgcca gccaatgcca
    13441 gtattaataa tggttgcgcc agccgcttcg atggcctgcg ccagttctac cgtttcggca
    13501 aaagtgccgc cgccttcgac caggtcgagc atcgacaaac ggtagataat aatgaagtca
    13561 ttgccgacgc gttcgcgcac cgcactcacc acttcaacgg caaaccgcat ccggttgtgg
    13621 taatcgccgc cccactggtc actacgctga ttggtgcgca gcgtcagaaa ttcgttgatc
    13681 aaataccctt cggaacccat cacctctaca ccgtcgtatc ctgcctcccg cgccagttgc
    13741 gcgcagcggg cgaaatcgtc gatcagttgc aggatctctt catgggtgag ttcgtgagga
    13801 acgaaacggt tgatgggggc ctgaaatgcg gagggagcga ccagatgcgg ttggtagctg
    13861 tagcgcccgg tatgcaaaat ttgtaaggca attttgccgc cttcctgatg tacggcttcg
    13921 gtaatggtgc gatggtgtgg gatctggctg gcgtcattga gcattgcgcc accttccatg
    13981 ccgacgcctg ttaaatctgg tgcaatgccg ccgctaacaa tcagcgccac gccgtgacgg
    14041 gcgcgttcgg cataaaacgc agccagccgt tcagcaccgt ccgggtattc ctccagcccg
    14101 gtgtgcattg aacccatcaa cacgcggttt tttaacgtgg taaaacctaa atccagcggg
    14161 gcgaacagcg acgggtagct cataaaattg tccagtatgt aaaataattg ttatgtggtc
    14221 ggatgagttc taatttagcc gtcgccggag aaaagggaaa aggggagtgt ggggtttgtg
    14281 atgggattca aaaataatac gcccattgga aatgccggat gcgacgcttg tcgcgtttta
    14341 tccaggttac ggaattgcac atgtagggcg gataaggcgt ccacgccgca tccgccagtg
    14401 gcgcggtgca gttgccggat gcaacgcttg ccgcgtttta tccaggttac ggaattgcac
    14461 atgtagggcg gataaggcgt c
//
LOCUS       DAOFQD010000116        14460 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.116, whole genome shotgun sequence.
ACCESSION   DAOFQD010000116 DAOFQD010000000
VERSION     DAOFQD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.116"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..754
                     /gene="dcuC"
                     /locus_tag="QBF46_003672"
     CDS             <1..754
                     /gene="dcuC"
                     /locus_tag="QBF46_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HDQ6675804.1"
                     /translation="AIAHFFWQRYLDKKEHISHEMLDVSEITTTVPAFYAILPFTPII
                     GVLIFDGKWGPQLHIITILVICMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANV
                     VMLLVAAGVFAQGLSTIGFIQSLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAP
                     FYAFVEMIPKLAHSSGINPAYLTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVV
                     KRTSVPVLVGLVIVIVATELMVPGTAAAVTGK"
     gene            complement(795..1475)
                     /gene="dpiA"
                     /locus_tag="QBF46_003673"
                     /gene_synonym="citB"
     CDS             complement(795..1475)
                     /gene="dpiA"
                     /locus_tag="QBF46_003673"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDQ6675805.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(1444..3102)
                     /gene="dpiB"
                     /locus_tag="QBF46_003674"
     CDS             complement(1444..3102)
                     /gene="dpiB"
                     /locus_tag="QBF46_003674"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDQ6675806.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKSVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            3482..4540
                     /gene="citC"
                     /locus_tag="QBF46_003675"
     CDS             3482..4540
                     /gene="citC"
                     /locus_tag="QBF46_003675"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDQ6675807.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            4555..4851
                     /gene="citD"
                     /locus_tag="QBF46_003676"
     CDS             4555..4851
                     /gene="citD"
                     /locus_tag="QBF46_003676"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDQ6675808.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            4848..5756
                     /gene="citE"
                     /locus_tag="QBF46_003677"
     CDS             4848..5756
                     /gene="citE"
                     /locus_tag="QBF46_003677"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDQ6675809.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            5767..7299
                     /gene="citF"
                     /locus_tag="QBF46_003678"
     CDS             5767..7299
                     /gene="citF"
                     /locus_tag="QBF46_003678"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDQ6675810.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            7303..7854
                     /gene="citX"
                     /locus_tag="QBF46_003679"
     CDS             7303..7854
                     /gene="citX"
                     /locus_tag="QBF46_003679"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDQ6675811.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDCEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELELSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            7829..8707
                     /gene="citG"
                     /locus_tag="QBF46_003680"
     CDS             7829..8707
                     /gene="citG"
                     /locus_tag="QBF46_003680"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDQ6675812.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            8758..10221
                     /gene="citT"
                     /locus_tag="QBF46_003681"
     CDS             8758..10221
                     /gene="citT"
                     /locus_tag="QBF46_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDQ6675813.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            10335..11141
                     /gene="rna"
                     /locus_tag="QBF46_003682"
     CDS             10335..11141
                     /gene="rna"
                     /locus_tag="QBF46_003682"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDQ6675814.1"
                     /translation="MKAFWRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            11371..11781
                     /gene="rnk"
                     /locus_tag="QBF46_003683"
     CDS             11371..11781
                     /gene="rnk"
                     /locus_tag="QBF46_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDQ6675815.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12012..13250)
                     /locus_tag="QBF46_003684"
     CDS             complement(12012..13250)
                     /locus_tag="QBF46_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDQ6675816.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEDCRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            13471..13899
                     /gene="uspG"
                     /locus_tag="QBF46_003685"
     CDS             13471..13899
                     /gene="uspG"
                     /locus_tag="QBF46_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDQ6675817.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14020..>14460)
                     /locus_tag="QBF46_003686"
     CDS             complement(14020..>14460)
                     /locus_tag="QBF46_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDQ6675818.1"
                     /translation="GIVEHVTLLEFAPEMKADQVLQDKLRSLKNVDIILNAQTTEVKG
                     DGSKVVGLEYRDRVSGDIHNIELAGIFVQIGLLPNTNWLEGAVERNRMGEIIIDAKCE
                     TNVKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYLIRTKTA"
BASE COUNT         3417 a         3852 c         3587 g         3604 t
ORIGIN      
        1 ggcgatcgcc cacttcttct ggcaacgtta tctggataaa aaagagcaca tctctcatga
       61 aatgttagat gtcagtgaaa tcaccaccac tgttcctgcg ttttatgcca ttttgccgtt
      121 cacgccgatc atcggtgtac tgatttttga cggtaaatgg ggtccgcaat tacacatcat
      181 cactattctg gtgatttgta tgctgattgc ctccattctg gagttcctcc gcagctttaa
      241 tacccagaaa gttttctctg gtctggaagt ggcttatcgc gggatggcag atgcgtttgc
      301 taacgtggtg atgctgctgg ttgccgctgg ggtattcgct caggggctta gcaccatcgg
      361 ctttattcaa agtctgattt ctatcgctac ctcgtttggt tcggcgagta tcatcctgat
      421 gctggtattg gtgattctga caatgctggc ggcagtcacg accggttcag gcaatgcgcc
      481 gttttatgcg tttgttgaga tgatcccgaa actggcgcac tcttccggca ttaacccggc
      541 gtatttgact atcccgatgc tgcaggcgtc aaaccttggc cgtacccttt cgcccgtttc
      601 tggcgtagtc gttgcggttg ccgggatggc gaagatctcg ccgtttgaag tcgtaaaacg
      661 cacctcggta ccggtgcttg ttggtttggt gattgttatc gttgctacag agctgatggt
      721 gccaggaacg gcagcagcgg tcacaggcaa gtaaatagta atgccggagg caagtttcct
      781 ccggctttat catgtcaccc actgtggtat atgcgttgtg gtctgccaac tttgccgtga
      841 acaatttcag caataatcag atggcggctg gcgcaatatt caagataacg cctggcagtg
      901 gtgcggctga tggtcagtgc ctgcgccacc gtttctgccg tatgttgcac accaggctct
      961 ttaaacagtt ttcgcaccgc gtttagcgtc aaggggtcaa tgccggtcgg tagctcgtcc
     1021 ttaggttcac cgcgagcata agcattaaac atctcatcaa tttgcttctg gctggcgcta
     1081 tcaatacttt ccagcatatg tttacgctgg cggaaacggg ttagcgtttg ccccagccgt
     1141 tcataggcaa tgggcttaat gagataatca aatacaccac aacgtacggc ttcagacacc
     1201 gtttccatat cgctggctgc agtggtaaac accacgtcgc cgggataatg cgcctgcacc
     1261 agttcatgca ataaattaat ccctctgccg tcaggaagat agttatcgag caagattagc
     1321 cccggcttaa aacgctcgat catcattcgg gcctgcgcca ggtttcccgc cagtaatatc
     1381 tgactgaatc cgggaatgtg acgaatatat tccgcatgca tctctgccag cggcgtttcg
     1441 tcctcaacga tcaatagggt taatggagct gtcattaggt ttcactttcg gaatatagat
     1501 tgaaaataag gtaccgcagg gatcattatc ttcgagagtg ataacaccac cgcagcgcgt
     1561 tacgtagctg gcaatcaagt acaacccaat gccatgttcg ccgggctcgt cagcacgcgt
     1621 actgaccccc tgctcaaata ttttgtctcg tagagactct ggaacgccgc agccctgatc
     1681 ggcgacttca atcaccacat catcgccttc atcgctgagg aataattcaa cgctcttgtt
     1741 tccttcatcg ctacgcaggc tggcttcgaa ggcgttatca agtaaattgc ccacaatggc
     1801 tgcaaactcg gtgctatcca gtcctggcgg cagttgcgaa agctggctac cggggacaat
     1861 gatcattttt agccccagtt cccgggcgcg ctgcacttta ccaaaaagta gccccgccac
     1921 ctggcgatcg gcaaacgcct cgcgcaggct gtcaataagc tgttgctggg cctgagactc
     1981 cccctgcacc atcgccagca cgcgatcata ctctttcatc tgcaacagac cattgagcgt
     2041 cgacatccaa ttgagatgct cgtgtcgcaa tgtacgaagg ctctcaacgt attgttttat
     2101 ttgcgtcagt tgcgcattga gggtggaaat ttcgtcttta ctgcgaaagc tgataatggc
     2161 ccccagcaaa tcatcacctg aacgaatagc ttcccgattg gcaataacgc tcagaccgtt
     2221 aaagttagcc accacatcct gacgtttttc atcaatctgt tcggtaaaga aatcggcggg
     2281 cctgaccact tcagcaatgg gtttacccaa ccattgccgt ccgggagagc tcagccccag
     2341 catctttctt gcgttacgat tgatggcggt aatgtaacca tgcggatcca ccgcaatcag
     2401 cccttcataa accgaactaa acagcgcctc ttgctgacgg accacgcgtg cgatttgctt
     2461 tggctccatg cccatcattt gccgacggat atgcgctgcc aggaaccacg acagcaacat
     2521 cagaatccct aacagcacga caaacacgcc tgccatcggt aataaaaact cagcccgcca
     2581 gctatcgatt ttactcacca ggtagccaat cgacaccacg ccgatgactt ttccatcgtc
     2641 atcaaagatt ggcgttttgg cgcgcatcgc catccccatt gaccctttcc cggtaatgaa
     2701 gtagctctcc cctttctcca gcgcgccctg tttggtgaac tgcataggat aaccaatttt
     2761 ctccggatta ggatggtaaa ggcggatcga gtgccggtcc ccaatcacca cataatcaaa
     2821 atcggtatcc ctttgtaatt tgttagcgat ggtcgccagc cgtttgtagt cacgcgtttt
     2881 caccgcagag atgacactgt cattggaggc aataattttc gcctgattca ttgccatgtc
     2941 gcgtacatga agcgttaaat agtcctcaaa actggccgta aaatattgcg ccagggctgc
     3001 aatgacaaat attgagaaca ccagaatcag caaaaagata cgcagcggaa atgccagtct
     3061 ttggaaaaat gcaaactgtt tattctcgtt aagctgcaac attgttttcc ttgcgggttg
     3121 tcccgatcct ctttgacgga tgtaattaaa atgttgaatc aaaatacata gatattaatt
     3181 atgatcgtgg caaattttat tcattaaatc aattaaatca cttaaacctg caggtcattt
     3241 cgtaattaca ttaattaaaa aacttaaaac cattaaataa ataaaaaacc actaactcta
     3301 tgtgaaataa atcaaaattt cacgctgcaa tactccttag gatgtatagc gaaaggagaa
     3361 aaagatatac ctcgatcacc cccctttctc ccaagtgaaa ataaaaggtt atcagtttgc
     3421 aacattgaac aacattcgtt gcaaatcgat aacaacatgc accttcagga tactatttat
     3481 tatgttcggc aatgatattt tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga
     3541 aatcgcccaa ttcctgcatg aaaatgattt gagcgttgac accacagtcg aagtatttat
     3601 taccgtaacc cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa atattattaa
     3661 atgcgttgct atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat tagccactga
     3721 actgataaac ctcgcctatg agcggcacag cacgcatctg tttatttata ccaaaaccga
     3781 atacgaggcg ctgttccgcc agtgcggttt ttccacgctg accagcgtac ccggcgtgat
     3841 ggtgctgatg gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt
     3901 tcgtcatcca gggaacaaga ttggctgcat tgtgatgaac gccaatccct ttacgaatgg
     3961 tcaccgttat ctgattcaac aagctgcggc acagtgcgac tggttgcatc tgtttttagt
     4021 caaagaagat tcttcacgct tcccctatga agaccggctg gatctggtgt taaaaggcac
     4081 cgccgatatt ccacgcctga ctgtgcatcg cggctccgaa tacatcatct cccgcgctac
     4141 gttcccttgc tacttcatta aagaacagag cgtcattaac cattgttaca ccgaaattga
     4201 tctgaagatt ttccgtcagt acctcgctcc cgcactgggt gtaactcacc gctttgtcgg
     4261 tactgaaccc ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct actggctgga
     4321 aacgccgact atctccgcac cgcccatcga actggttgaa attgagcggc tgcgttacca
     4381 ggagatgccg atatccgctt cccgggtacg tcaactgctg gcgaaaaacg atctcacggc
     4441 tatcgcgccg ctggtccctg cagtcacgct gcattatttg cagaacctgc ttgagcactc
     4501 ccgccaggac gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa
     4561 ataaaccagc ccgccgttgc aggcaccctt gagtctgggg atgtgatgat acgcatcgcc
     4621 ccactcgata cgcaggatat cgacctgcaa atcaacagca gcgttgagaa acagtttggc
     4681 gatgcaattc gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag
     4741 ctgaatgtcg atgacaaagg cgcactggac tgcattttac gtgcacgact ggaagccctg
     4801 ctggcacgcg ccagcggtat cccggctctg ccatgggagg attgccaatg atttccgctt
     4861 cgctgcaaca acgtaaaact cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg
     4921 ccgcgatggt cagcaactcc ttcatctacc cggctgatgc cctgatgttt gacctcgaag
     4981 actccgtggc attacgtgaa aaagacaccg cccgccgcat ggtttatcac gcgctgcaac
     5041 atccgctgta tcgcgatatt gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg
     5101 gtgttaacga cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa
     5161 ccgataccgc tcaggatgtt ctggatattg aaaaagagat cctgcgtatc gaaaaagcct
     5221 gtggtcgtga acccggcagc accggcctgc tggcggcgat tgaatctccg ctgggcatta
     5281 cccgcgcagt ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag
     5341 aagactatgt gcgcaacctg cgtacagaac gctccccgga aggaactgaa ctgctgttcg
     5401 cacgctgttc cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct
     5461 attccgacgc taacaacgaa gccggattcc tgcaagaagc cgcccacatc aaacagctgg
     5521 gctttgacgg caaatcgctg atcaacccgc gtcagattga tctgctgcac aacctctacg
     5581 caccgaccca gaaagaagtg gatcacgccc gccgcgtcgt agaagccgct gaagccgccg
     5641 ctcgcgaagg cctcggcgtg gtttccctga acggcaagat ggtggacggt ccggttatcg
     5701 atcgcgcccg tctggtgctc tcccgtgcag aactttccgg catccgcgaa gaataaggca
     5761 atcaaaatga cgcagaaaat tgaacaatct caacgacaag aacgggtagc ggcctggaat
     5821 cgtcgcgctg aatgcgatct tgccgctttc cagaactcgc caaagcaaac ctaccaggct
     5881 gaaaaagcgc gtgatcgcaa actgtgcgcc aacctggaag aggcgattcg tcgctctggt
     5941 ttacaggacg gcatgacggt ttccttccat cacgctttcc gtggcggtga cctgaccgtc
     6001 aatatggtga tggacgtcat cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc
     6061 tccctgagtg attgccatgc gccgctggta gaacacattc gccagggcgt ggttacccgc
     6121 atttatacct ctggcctacg cggtccactg gcggaagaaa tctcccgcgg tctgctggcg
     6181 gaaccggtgc agatccactc tcacggcggt cgtgtgcatc tggtacagag cggcgaactg
     6241 aatatcgatg tggctttcct cggcgtcccg tcctgtgatg aattcggtaa tgccaacggc
     6301 tacaccggta aagcctgctg cggctccctc ggctatgcaa tggttgatgc cgacaacgca
     6361 aaacaggttg tgatgcttac cgaagaactg ctgccttatc cgcataatcc ggcaagcatt
     6421 gagcaagatc aggttgattt aatcgtcaaa gttgatcgcg ttggcgatgc tgcaaaaatc
     6481 ggcgctggtg ctacccgtat gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct
     6541 gcggatgtga ttgtcaactc tggctacttc aaagaaggtt tctccatgca aaccggcacc
     6601 ggcggcgcat cgctggcggt aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt
     6661 cgcgccgact tcgcccttgg cggtattacc gcgacgatgg ttgacctgca cgaaaaaggt
     6721 ctgatccgca aactgctgga tgtgcagagc tttgacagcc atgctgcgca atcgctggcc
     6781 cgtaacccca atcacatcga aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc
     6841 gcatcggttg atcgtctcga cgtggtggta ctgagcgcgc tggaaattga cacccagttc
     6901 aacgttaacg tgctgaccgg ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc
     6961 gataccgcaa ttgcctctgc gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt
     7021 ccgactctgg tggataacgt gctgacctgc atcaccccgg gctccagtgt cgatattctg
     7081 gtcacagacc acggtatcgc agttaacccg gcacgtccgg aactggcaga acgtctgcag
     7141 gaagcgggca ttaaagtggt ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc
     7201 ggtgaaccac agccgattga attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat
     7261 ggctcggtga tcgatgttgt gcatcaggtg aaggaataag ccatgcacct gcttcctgaa
     7321 ctcgccagcc accatgcggt atcaattccc gagctgctcg tcagccggga tgaaaggcaa
     7381 gcacggcaac acgtctggct caagcgccat cctgttccac tggtctcctt taccgtggtt
     7441 gcgcctgggc cgattaaaga ctgcgaggtc acacgccgaa tttttaatca tggcgtgaca
     7501 gccttgcgtg ccttagccgc aaaacagggc tggcaaattc aggagcaggc tgcactggtt
     7561 tccgccagcg ggccggaggg catgttgagc attgccgccc cggctcgcga cctcaagctc
     7621 gccaccattg agcttgaact tagtcatcct ctcgggcggt tatgggatat cgatgtcctg
     7681 acgcccgaag gcgaaattct ctcccgccgc gactattcac tgccgcctcg ccgctgcctg
     7741 ttgtgcgagc aaagcgcagc cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta
     7801 ctcaaccgca tggaggcact gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac
     7861 aaagcttgcg acgtcattaa tcgatgagta cgccctcttg ggctggcgcg ccatgctgac
     7921 tgaagtcaat ctgtcaccga aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa
     7981 agatatggcg ctggaagatt tccaccgcag cgcgctggcg attcagggct ggttaccccg
     8041 tttcattgaa tttggtgcct gtagtgcaga aatggcaccg gaagcggtac tccacggatt
     8101 acgcccaatt ggtatggctt gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac
     8161 gcataaaggc agcatttttt ctttagggct gctatgtgcg gcaattggcc gtttgcttca
     8221 actcaaccaa ccggtaacgc caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg
     8281 cctgaccgat cgagaactgc gtaccaataa ttcacaactg acggcaggtc aacggttgta
     8341 ccagcagctt ggccttaccg gcgcacgcgg tgaagccgaa gcgggttatc cgctggtgat
     8401 caatcacgcc ttgccgcatt acctcactct gctggatcag gggttagatc ctgaactggc
     8461 attgctcgat accttgctcc tactgatggc gatcaacggc gataccaacg ttgcatcgcg
     8521 cggtggcgag gggggcctgc gctggctaca gcgcgaggcg caaacattat tgcaaaaagg
     8581 gggcattcga acccccgccg atctcgatta tctccggcag ttcgacaggg agtgtatcga
     8641 acgaaacctc agtccaggcg gcagtgccga cctgctgatc cttacctggt ttttagcaca
     8701 gatttaatta tttaagcact tgataaattt ggaaatatta attttcggag aacccgtatg
     8761 tctttagcaa aagataatat atggaaacta ttggccccac tggtggtgat gggtgtcatg
     8821 tttcttatcc ctgtccccga cggtatgcca ccacaggcat ggcattactt cgctgtgttt
     8881 gtggcaatga ttgtcggcat gatcctcgag ccaattccgg caacagcgat cagttttatt
     8941 gcggttacta tttgcgttat tggcagtaat tacctgctct ttgatgccaa agaattagct
     9001 gacccagcgt ttaatgcgca aaaacaggcg ctgaaatggg gtctggctgg tttttccagc
     9061 accacggtat ggctggtatt tggcgcattt atttttgcat tagggtatga agtttccggg
     9121 ttaggtcgtc gcattgccct tttcctggtg aaattcatgg gcaaacgcac gctgacgttg
     9181 ggttatgcga ttgtcattat cgacattctg ctggcaccgt ttacaccgtc caacaccgcg
     9241 cgtaccgggg gtacggtttt tccggtcatt aaaaacctgc cgccgctgtt taaatcattc
     9301 cccaacgatc cgtccgcgcg tcgtattggc ggctatttga tgtggatgat ggtcattagt
     9361 accagtctga gttcgtccat gtttgtcacc ggtgcggcac caaacgtgct gggtctggag
     9421 ttcgtcagca aaattgccgg tatccagatt agctggttgc agtggttcct ctgcttcctg
     9481 ccggttgggg ttatcttgct tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg
     9541 gaaatcacac acagtgaaga agtggcaacc tgggcgggtg atgaactgaa aaccatgggt
     9601 gcgctgacac gcagagagtg gacactgatt ggccttgtat tgctcagctt aggtttgtgg
     9661 gtttttggca gtgaagtcat taatgctact gcagttggtc tgctggcagt ttcgctaatg
     9721 ctggccctgc acgttgtgcc ttggaaagat attacccgct ataacagcgc atggaacacg
     9781 ctggtcaacc tggcaactct ggttgtgatg gctaacggcc tgactcgttc tggttttatt
     9841 gactggttcg ccggtaccat gagtacgcac ctggaaggat tctcaccaaa cgcaacggtg
     9901 attgtactgg ttctggtgtt ctactttgca cactacctgt ttgccagcct gtctgcgcac
     9961 accgcaacca tgctgccggt tattctggcc gttggtaaag gtattccggg cgtaccaatg
    10021 gaacaactgt gtatcctgct ggtgctgtct atcggtatca tgggctgtct gacgccgtat
    10081 gcaaccggtc ctggggtgat tatttacggc tgtggctatg tgaaatcaaa agattactgg
    10141 cgtcttggcg caatcttcgg ggtgatttac atctctatgt tgctgttggt tggctggccg
    10201 attctcgcca tgtggaacta atacgttcgc caaacgctga ataaatcatc acgcccgcca
    10261 ggtaagccca cctggcgggc ttttttatga tttaatagat agtattcagt gtatgtatga
    10321 gttccacacc cattatgaaa gcattctggc gtaacgccgc gttgctcgcg gtttctctgc
    10381 ttcccttatc ttctgccaac gccgtagcgt tgcaggcaaa acagtatggc gattttgatc
    10441 gctatgtcct ggccctctcc tggcaaaccg gattttgcca gagtcaacac gatcgaaatc
    10501 gtaacgaacg agatgaatgt cgcctgcaaa ccgaaacgac caacaaagct gattttctga
    10561 ccgtacatgg tctgtggcca ggattgccta aatcggttgc tgcccgtggt gttgatgaac
    10621 gccgctggat gcgcttcggt tgcgctactc gcccaatccc gaatctacca gaagcacgcg
    10681 ccagccgaat gtgttcatcg ccggaaaccg ggttatcact ggaaacggcc gctaaactaa
    10741 gtgaagtcat gccaggagct ggcggacgtt cctgcctgga acgctacgaa tatgccaaac
    10801 acggtgcctg ctttggtttt gatccggacg catacttcgg tacgatggta cgcctgaatc
    10861 aagaaattaa agagagcgaa gccggaaaat tccttgcgga taattacggt aaaacagtga
    10921 gccgccgtga ctttgacgcc gcctttgcca aaagctgggg aaaagagaac gtgaaagcag
    10981 ttaagctaac gtgccagggt aaccctgcgt atttgactga aattcagatc tcgatcaaag
    11041 ctgacgccat caacgctccg ctttctgcaa actcattctt gccacaacca cacccaggca
    11101 actgtggcaa aacctttgtg attgataaag tgggttatta atcatctgat ttacaaaaca
    11161 cgctaattcg tcttccataa accgtgacga ttaagaatta tacataatgc gctgcattga
    11221 ggaattccct gaatgtgacg caaatcactt caaagagtga gcaacagtca gtatcatcat
    11281 gtgagttaaa ccctcgccgc ctgacggtga gggttttctt ttgggtatgt ttatctgcga
    11341 cactcgcagt accgacgaca tggagtaaaa atgtccagac caactatcat cattaacgac
    11401 ctggatgccg aacgcatcga tattctgctg gagcaacccg cctatgctgg tttgccaatc
    11461 gccgacgcgt taaacgcaga gttggatcgc gcccaaatgt gttcgccaga agagatgcca
    11521 cacgacgtgg tgacaatgaa cagccgggtt aaattccgca atcttagcga tggcgaagtg
    11581 cgtgtgcgca cgctggtgta tccggcaaaa atgaccgata gcaatactca gctttccgtt
    11641 atggctccgg taggtgccgc actgctgggg ctgcgcgttg gcgattccat tcactgggaa
    11701 cttccgggcg gcgttgcaac ccaccttgaa gtgctggaac tcgaatacca gccagaagct
    11761 gctggcgact acctgcttta atcccatctg cacaagcgat aactcgcttt gcccagagga
    11821 tgcactcgca tcctctttac gcaaaatcag caatttttcc tggttctcac tgttcacttt
    11881 tgctgcaaaa ttattccgtt tcgtttgtgg gaataaccat ggcagaagca ttttacatcc
    11941 tgataggatt tctgattatg gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc
    12001 actcctgacg atcatattgt tccccccggc atcgcattca ccagacctga aaccgccttc
    12061 tgcgccgcct ctgcgctttg tgcaccgggt accagaatca ccttacggca atcctcttca
    12121 cgtttttcga aaatctcata tccccgggcg gcctcttcaa acggcatata gtgggtaaca
    12181 atttcttctg gtttcagtaa tcctttctca attaacggta ataattctcc cagccatgcg
    12241 tgaacgtggg tctgtcccat tttaaacgtc aaccctttat caaaggcgtc gccaaacagg
    12301 aaaccgtgaa taaatccagc gtagacgccc ggtacgctaa caatgccgcc acgcctgacc
    12361 gccgcaatac actggcgcaa cgctttaccg ctgctgccct ccagtttcag gttagtcagc
    12421 accgtttccg tggtgctgcc tttcgcttca aaaccgacgg cgtctattac tgcatccacg
    12481 ccccggtgac ctgccgtttg ttcaataatt gactgtgccg gatcgctatc ttcatcgaaa
    12541 ttaatcggga tcgcgccgta gcggtcagcg gcgaaacgca agcggtaggg atgatgatca
    12601 acaacaaaaa tctgttccgc accgagcaac cgtgcacagg cgattgtcaa caatcccact
    12661 ggaccggcac catagactgc aacgcttgaa ccttgttgga tctgcgcatt ttttgctgcc
    12721 tgccatgccg ttggcagaat atcagaaagg aaaagggctt tatcatctga aagcaaaggc
    12781 ggtactttaa acggccccac attcccttta gggacgcgga catattccgc ctgcccacca
    12841 ggaacgccgc catacaggtg actataacca aacaatgccg cgggagctgg tatctgtttt
    12901 ttattgagcg cagcgccttt acccgcattg gtattttcgc aggcggcata ttgctgtaat
    12961 cgacagaaaa aacagtcgcc acaagcaatg acaaacggaa ttaccacccg gtcgcctttt
    13021 tgcaaatttt ttacgtcctt tccggtttca acgacttccc ccataaattc atgaccaaaa
    13081 atatcgccat gtttaacctg gggtattttg cctcgataaa gatggaggtc agagccacag
    13141 atcgccgttg ccgtaatacg cagaataata tcatctgcct gttcaatgcc cggatcggga
    13201 acattttcta cctgaacgtg atgtgggccg tgataagtca atgctttcat gcaacctcca
    13261 gagacagttc aaaaaagagt cagtctttaa gggtagctat cacagcggga aaacgggcgg
    13321 taccgcaggt tttgggagaa ttcctggtgt tcagtgagaa tatattaatc acagaaaccg
    13381 ttcgtaagga aaaagaacca gacaaatatc agggataaat attataatca tggcgaatga
    13441 tttgttcatg attaacaggg agaaagggtt atgtataaga caatcattat gccagttgat
    13501 gtatttgaaa tggaattgag cgacaaagct gttcgccacg ctgaattcct cgcccaggat
    13561 gacggagtta ttcatctact tcacgtacta cccgggtcag ccagcctgag cctgcaccgt
    13621 tttgccgctg atgtgcgtcg ttttgaagag catctgcaac atgaagcaga agaacgtctg
    13681 caaacgatgg tcagccactt caccatcgat ccttcccgca ttaaacaaca tgtccgtttt
    13741 ggtagcgtgc gggatgaagt caatgagttg gcagaagaac tgggggctga tgttgtagtt
    13801 attggttctc gcaacccatc gatttcgacc catctgttag gttctaacgc ctcgagcgtg
    13861 atccgccatg ccaatctgcc ggtgctggtt gtgcgttaat tgccagaata aatatcccgc
    13921 cctgcccgga gccatcccgg cagggctttt taatgccata aaaaagccgc caggtttgac
    13981 cccggcggct aagcaattgc aggtgaatct tacttcttct tatgcagttt tggtgcgaat
    14041 caggtagtca aaagcactca gagaggcttt ggcaccttcg ccagtggcga tgatgatctg
    14101 cttgtacgga accgtcgtac agtcacccgc tgcgaatacg cctttgacgt tggtttcgca
    14161 tttcgcatca atgataatct cacccatgcg gttacgttcg actgcgcctt ccagccagtt
    14221 ggtgttcggc agcagaccaa tctggacgaa aataccggcc agttcgatgt tgtgaatatc
    14281 gccgctgaca cgatctcgat attccagacc aacgactttg ctgccgtcgc ctttcacttc
    14341 cgtggtttgc gcattcagaa taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag
    14401 cacctggtcg gctttcattt ctggcgcaaa ttccagcaga gttacgtgct caacgatacc
//
LOCUS       DAOFQD010000117        14288 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.117, whole genome shotgun sequence.
ACCESSION   DAOFQD010000117 DAOFQD010000000
VERSION     DAOFQD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.117"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            95..451
                     /locus_tag="QBF46_003687"
     CDS             95..451
                     /locus_tag="QBF46_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDQ6675819.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(502..1398)
                     /gene="yhaJ"
                     /locus_tag="QBF46_003688"
     CDS             complement(502..1398)
                     /gene="yhaJ"
                     /locus_tag="QBF46_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDQ6675820.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            1503..2204
                     /locus_tag="QBF46_003689"
     CDS             1503..2204
                     /locus_tag="QBF46_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDQ6675821.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2227..2391
                     /locus_tag="QBF46_003690"
     CDS             2227..2391
                     /locus_tag="QBF46_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675822.1"
                     /translation="MSKKLAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLVEDEATA"
     gene            complement(2452..3762)
                     /locus_tag="QBF46_003691"
     CDS             complement(2452..3762)
                     /locus_tag="QBF46_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDQ6675823.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3790..5121)
                     /locus_tag="QBF46_003692"
     CDS             complement(3790..5121)
                     /locus_tag="QBF46_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDQ6675824.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5396..6760)
                     /gene="tdcG"
                     /locus_tag="QBF46_003693"
     CDS             complement(5396..6760)
                     /gene="tdcG"
                     /locus_tag="QBF46_003693"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDQ6675825.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6832..7221)
                     /locus_tag="QBF46_003694"
     CDS             complement(6832..7221)
                     /locus_tag="QBF46_003694"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDQ6675826.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7235..9529)
                     /gene="tdcE"
                     /locus_tag="QBF46_003695"
     CDS             complement(7235..9529)
                     /gene="tdcE"
                     /locus_tag="QBF46_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDQ6675827.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9563..10771)
                     /gene="tdcD"
                     /locus_tag="QBF46_003696"
     CDS             complement(9563..10771)
                     /gene="tdcD"
                     /locus_tag="QBF46_003696"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDQ6675828.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10797..12128)
                     /gene="tdcC"
                     /locus_tag="QBF46_003697"
     CDS             complement(10797..12128)
                     /gene="tdcC"
                     /locus_tag="QBF46_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDQ6675829.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12150..13139)
                     /gene="tdcB"
                     /locus_tag="QBF46_003698"
     CDS             complement(12150..13139)
                     /gene="tdcB"
                     /locus_tag="QBF46_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDQ6675830.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13238..14176)
                     /gene="tdcA"
                     /locus_tag="QBF46_003699"
     CDS             complement(13238..14176)
                     /gene="tdcA"
                     /locus_tag="QBF46_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDQ6675831.1"
                     /translation="MSTILLPKTQHLIVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3688 a         3827 c         3340 g         3433 t
ORIGIN      
        1 aaacctttat aacatccgaa aaagaaagat acgaatagca tttacacaat acccggaata
       61 gactttcatc acttttaatt aaagggatgt ttttatgcag tggtatttgt ccgtattaaa
      121 aaattatgtt ggtttctctg gtcgcgcacg tcgtaaagag tactggatgt ttactctgat
      181 taacgccatc gtcggcgcta ttatcaatgt cattcaattg attttaggtc tggagcttcc
      241 atatctgtct atgctttatt tgctggcaac cttccttccc gtccttgcgc ttgctatccg
      301 tcgcttacac gataccgacc gttcaggtgc ctgggccttg ctcttttttg ttccgtttat
      361 tggctggctc gtgcttctgg tttttttctg cacagaaggt acttctggca gcaatcgtta
      421 cggaaacgat ccgaagtttg gttcaaatta atcttagaat tggggcgata tttcgcccct
      481 ttttattaac aataataaat attattttcc gttaaaaagt ttgggaattt cccgcagaca
      541 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
      601 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatagg
      661 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
      721 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
      781 agcggtatcc gccaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
      841 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
      901 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacaatatc
      961 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     1021 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     1081 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     1141 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccaaca acatcctccc
     1201 gacattggtg aatttggtac gatggcccga gcggtcaaac agcaccacat ccagctcttc
     1261 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgccgc
     1321 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     1381 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     1441 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     1501 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     1561 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     1621 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     1681 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gatagcgaag
     1741 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     1801 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     1861 acgcctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atgggcaagc
     1921 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc cagcaagtgt
     1981 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     2041 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     2101 cggcgctgac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     2161 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     2221 atgatgatga gtaaaaaatt ggccaaaaag cgccagccgg tgaagcccgt ggtggcgaaa
     2281 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     2341 atcgtcgcgg atgctgaaac gcgtttagtc gaggatgaag ctaccgcgta aagacaagtt
     2401 gccggatgcg gcgaaaacgc cttatccggc acaataccca aaacctggcg tttatctggc
     2461 cttgctcgcc ataatctcga taatctgccg atcggtttgc tgcatcgaat ggcttgctaa
     2521 cgcacacagg ttggcaatcg actgctcaac atcatgcgcc acgatccctt cattgccggt
     2581 cacggcggta tcatccagcg ccattaacac cgctttccac gcagccgaag cactggtcga
     2641 aaccttcatc gcacagctgt tcgacgctcc atcgcaaatc atgccgctga catcgccgat
     2701 catactgctg atcgccatcg agatagtttc ataacgccca tccaccagcc atgccatccc
     2761 ggcggcggcc cccattgctg cggtcgttgc ggcacaaagt gcagacaaac gcggtaactg
     2821 gttatggatg taaattgcgc tcaaatgcga aagcatcagc gcacgcgcca gccgttcatc
     2881 atccgctccg aagtgttctg ctaccaccac cacaggcatt gttgcggtga tcccctggtt
     2941 acccgagcca gagttactca ttgccggaag cgtagcgccg cccatacgcg catcggatgc
     3001 cgcgctggta cgaatcacaa tggatgaaga gagatctttc gccagcaaac cgcgcgcgca
     3061 ctgtttttcc agcgtcgcgc caatatgcag cccccactta ccgctcaaac cttcctgcga
     3121 taacgcacaa tttagcttcg cagaatcgag aataaagcgg atcgccgcaa acgggacttc
     3181 attgacgaac ttcaggatct cagccagcgt cgttctggaa agcaccgtca gcggagactc
     3241 ttgctcgccc tctgccacac acgcctgctg ggtaaacacc acaccatcgt gcgtctcaat
     3301 atgcacaatg ttggtatgcc caccgacgat ggtgacacac gcccacttct caccgttcca
     3361 gactttggcg cgtgagaaga ggatttcatt gcaaggttcc tggatcttaa cggagacttt
     3421 ccccgccgcc agcagtgctt tggcatcggc aattgcctgc gcagttgcgt ctttcagcac
     3481 ttccagcccg gcgttggcat ttccacctaa cgcccccagc gccgccgcaa tcggcagccc
     3541 caccattccc gtgccgggaa cggtgacgcc cagaccgttc ttcatcagat ttggcgaaac
     3601 ccaggcttct acacgttcaa ccggaccttc cagttctgcc gcagcaaccg ccgccgccag
     3661 cgccagtgaa atcggttcag tacatcccag cgccggtttt acttcctcct gaacggcgag
     3721 gatgtaacgc tgccataacg gatttaaagt cgaatcaaac atattaaaaa ccttaaaatt
     3781 tcaggtaaat caagaaaatg ccaggaacgg agaaacacaa agcaacaaac cagtgacgat
     3841 aatcaggtac agagacatcc ctttgtattt gtgcaatgcc ggtactttgt aaaccagcca
     3901 cgccgggatc aggcacccta ccatgccgaa aatcgggcta cagatagagg tgaagctcaa
     3961 caccggtgcg ttcagtacga tggcgctcca ggccagcaaa atggcgaaaa tcatgatgcc
     4021 gcgctgaaca agattttcgt taatcttctc ggcaggcatc ttgcgacgca ggatgttcat
     4081 tacgatccct tgcgttgctt cgcgaaagcc taaatagacg ccaaagaatg cagtcatgac
     4141 agcaaagata ttgagaatga cgctgaccac tttcacccac gctgcgccgt caccgctaat
     4201 aaactgtgcg gcaatcgcca gcgcggaaat attctgctcg taggctttta ccgcttcgtc
     4261 atgtcccatc gccagcgtga acgacacggc gtagaaaaag acggtgacaa acaaaatgcc
     4321 aaacgcgata ttcattgccc gcaatgcttt atgccgcgcc acttcaatgg atttttcccg
     4381 cgagcgataa gagatcacca tcgggcttaa cgtctgaata aacagaatcg acgtcagggt
     4441 aaacggcagc gtaataatgg cgtttttcac cagcagcccc agcggcggta gcgaaccgac
     4501 gttatacaga tgccacattc ccaccatcga cacgcccagc gccgcgacca ccagcagctt
     4561 ggtcagcacc atgccggtcg aaattttgaa taacaatttc tcgccgcgtg aggagatcgc
     4621 caccagaatg caaatcagta ccagaccata aaagggactg tctgacagca acccttccgt
     4681 cacgccgaag gtatgcaggt aggaagcact atcgttggtg atggcggtgg aatagacgaa
     4741 catccaaatc accagcatta cgaaatagag cgcacctaac aggatgcccc agtttttacc
     4801 taaataaccg ctaatgacac tcgggtaatc tttacattct ggtgattctg ccagcgtatt
     4861 aataaacaac cgctgaaaca gatacattgc cgggtaacca atcaccgatg agagcaaaaa
     4921 tacccacaat cccatcaaac cgacctgcac cgggagaaaa acaatccccg cgccaatcgc
     4981 catcccgata ctcataatca cccagccggt gtcagtactg tcgaatttga tcgcttctcg
     5041 ccactcgctc tcactcattc cagcacgacc cgccggggtc gaagcgtctg caatgacgcc
     5101 tttattcgat gcaatttcca taatttctcg ctcaaaattt tgtagggctt attatttttt
     5161 tccgagccgc atcaaggcga tatgcggtct acgtttttgc aggcgagtta ttagaataaa
     5221 gaaatgatac gcgcgaggtc agagaaaatc ttcacaatca agactttgaa ataacggaga
     5281 tggataagaa ttttctacta aattaatcgc aagagaatat tcattctcta tttgcggcag
     5341 atcacaaaaa aaaggtgcac atttgtgcac ccaaggatga aagctgacag caatgtcagc
     5401 cgcagaccac tttaatggcc agtcctccgc gtgatgtttc gcggtattta tcgttcatat
     5461 ctttgccggt ttcatacatc gtctcgatca ctttatcgag tgaaacacgc ggtgccgagg
     5521 tgcggcgcat cgccatccgc gcggcgttta ctgctttcac ggcattaatg gcattacgtt
     5581 caatgcacgg gatttgtacc tgtccggcaa ccggatcgca ggtcagccca aggttatgct
     5641 ccatcgcgat ttccgccgca ttgcatacct gcgccggact gccgcccagt agttcagtta
     5701 accctgccgc cgccattgaa caggccacgc caatctcccc ctgacagccg acttccgcgc
     5761 cagagatgga ggcgttcatt ttatacagcg cgccaatagc ccccgcggcc agaaaatagc
     5821 gggcaattga ccgctcgttt accggacgac ggaacttatc gtaataagcc agtactgccg
     5881 gaataatgcc gcacgcaccg ttagtcggtg ccgttaccac gcgcccgcca gctgcgtttt
     5941 cttcactaac cgccagcgcg tacatgttga tccagtcgat gacattcatc ggatcgttag
     6001 agatgttatc gctggaaacc agctgacgac gcagcgctac ggcacggcgc ggcacattga
     6061 gcggaccagg cagcacgcct tcagtgttca tcccacgttc aataccgtca tgcatcactt
     6121 gccagatacg ggcaaaaccg gcgtcaattt ccgctttgct gcgcagcgct agctcgttgt
     6181 gcatcatcag accagatata gacaggccgt tgtaatcaca cattttcagc agttcacctg
     6241 ctgagtggaa atcgtaaggt acggacgttt cgacatcgtg cgacaggccg aagtgttctt
     6301 cttcgacaat aaacccgccg ccgacagagt aataggtttt acttaatagc tcttcctgtc
     6361 ccttccaggc agtgatccgc attccgttct catggcgagg caacatttcg ggatggaaga
     6421 taatgttctt tgctacagga aaatcaacaa tatgcgcacc agatgccact ggcagccgcc
     6481 cgctgcgcgt tactaactct ataaatgcag ggatctcatc aatgacaaca tcctgcggac
     6541 tgtttcctgc cagtcccatg atgatggcga catccgtggc atggcctttg cccgtcagtg
     6601 acaacgaccc gtacagatcg accacaatat ggctcgtcgc ggttaataag ccgctacttt
     6661 ccagccgatc aataaaactt tttccggcat tcattggccc cacggtatgc gaactggagg
     6721 gaccaatccc aattttgaaa atatcgaatg cactaatcat atccacaccc tcggattgcc
     6781 gttcagtgaa gtggagcgga acgaccttac gaccgtcccg ctcacgaggc tttacgcact
     6841 acgtactgcg atggcttcaa tttccagctt cacatctttc ggcaaacgcg cgacctgcac
     6901 acagctccgg gtcggatagg tcgcctgatg ctcatcgaag aactgcttat agacttcgtt
     6961 gatggtggca aaatcattca gatcggtgat aaacacggtc atcttgatga tatcgcccac
     7021 gctcagcccg gcagcaacca cgatcgcttt gacgttttcg aggcttaaac gcgcctgatc
     7081 ttgcacatca gccgggatct caccggtctg tgggcaaacc ggtatttgcc cggaggtgaa
     7141 gaccatgctg cctaaatcaa cgccctgaac ataagggccg attgcgcctg gggcacgttg
     7201 cgtttcgata atctttttca tacatcctcc ggcgtcagag cgcctgggta aaggtacgtg
     7261 aaataacatc ctgttgctgt tcacgggtca gtgcgttgaa gcgcacggcg tagccagaga
     7321 cacggattgt caggttagga tatttttccg ggtgctcgat ggcatccagc agcatttccc
     7381 gattcattac gttgacgttg aggtgttgac cgccttcgac atccgcttcg tggtggaaat
     7441 acccatccag caggccgaca aggttggttt tacgtactgg atcttctttg cccagcgccg
     7501 caggaacgat tgagaaggtg tacgagatcc catctttggc gtaggtgaac ggcagtttcg
     7561 ccaccgacgt caatgaggcc acggcacctt tgcggtcacg accatgcatc gggttagcgc
     7621 ccggcgcgaa cggtgttccg gcgcgacgac cgtccggcgt attaccggtt ttctggccgt
     7681 acaccacgtt agaagtgata gtcagaatcg actgggtagg gacggcgttg cgataggttg
     7741 gcagcgcttt aattttcttc ataaagcgtt caaccaggtc gcaggcaatg ctgtctacgc
     7801 gctcgtcgtt gttgccgtac tgcggatatt caccgtcgat ttcaaagtcc accgccaggc
     7861 cgttttcgtc acggattggt ttcacgcggg catatttgat ggcagacagg gagtccgtcg
     7921 ccaccgacag gcccgcgatg ccgcatgcca tagtgcgata gacatcacga tcgtgcagcg
     7981 ccatcagcga agcttcgtag ctgtacttgt cgtgcatgta gtgaatgata ttcagcgcgc
     8041 tgatgtactg caccgccagc cagtccatga agtgatcgag gctgtccatc actttgtcgt
     8101 aatccagcac gtcgtccatc agcggtgctg ttttcggccc gacctgaatc ttcagcttct
     8161 cgtccacccc gccgttaatt gcgtagagca gcgttttcgc caggttagcg cgtgcaccaa
     8221 agaactgcat ttgcttacca atcaccattg ggctgacgca gcaggcaatc gcgtaatcgt
     8281 cgctgttgaa gtcagtacgc atcagatcgt cattttcata ctgcaaggaa gaggtgacga
     8341 tcgacacctg cgcggcatat tttttgaagg caatcggtaa ttcttccgac caaagaatgg
     8401 tcaggttagg ctccggtgcc ggccccatag tgtgcagggt gtgcaaatag cggaaggagt
     8461 ttttggtcac cagcgtacga ccgtccagcc ccatcccgcc gatcacttcc gtcgcccaga
     8521 ttgggtcgcc ggagaacagc gaatcaaatt ccggcgtacg caagaagcgc accatacgga
     8581 tcttcataat gaagtgatcg atcagttcct gtgcctgctg ctcattgagt acgcctgctt
     8641 taaagtcgcg ttcaatgtag atatcgagga acgatgccgt gcgccccagc gacatcgcgc
     8701 cgccgttctg tgatttcact gccgccagat aagcgaagta gagccactgc accgcttcct
     8761 gcgcattctg cgccgggcga gagatatcaa agccatattt cgccgccatt tcctgaatct
     8821 gcaacagcgc atgacgatgc tctgccagct cctcacgcag acggatggtg gcttccagat
     8881 cctcgccttt ttccagacga gactggagat cggcaaattg cagttcgcgt tcacgcacca
     8941 gataacgaat gccatacagc gctacgcggc gatagtcacc gataatgcgc ccacgtccat
     9001 aaccatctgg taaaccggtc agtacgccag atttacggca gcgcagcata tccggtgagt
     9061 aaacatcaaa tacgccctgg ttatgggttt tacgcagatc ggtaaacaga tattcaaatt
     9121 cactgtccat ttctcggcca taggcgtgga atgaactttt aatcatatta atgccaccga
     9181 acgggtgtag cgcacgtttc aacggcgcat ccgtttgcag gccaacaatt ttttccagcg
     9241 gctggttaat atatcccgca tcatgagcgg taattgtggt ggcaatattg gtatcgaaat
     9301 caaccggcgc gtgggttgca ttttcgatac ggatgccttc cattactttt tcccacaatt
     9361 ccgtggtggc aggcgtcgct tcggcgagga aagattcatc gccttcatac ggtgtatagt
     9421 tatgttgaat aaaatcgcgg acattaattt cgtttttcca gtccgtacct ttaaagccaa
     9481 gccatgcgtc ggcgtacagc ttatcgctgg tatcaatatc taccttcatg aaaaataatc
     9541 tctctacaat acttcaacta aattatgcaa attctgcggg cgcgttaact ttgcctaaat
     9601 gaatggcatc caaagcaatc attttttctt cgttagtcgg aataacggca caaatgacac
     9661 gcgcattttc actggaaaca attcgctctc caaaagagtt agatcgatta ttcatttctg
     9721 tatcaatcac tacgcctaat acagccaaat gttccatgac cagacgacga attaaacttg
     9781 aattctctcc tattccgccg gtgaatataa ttccatccag gcgacgtaat gaagctgcgt
     9841 gtccggcaat atgacgggca attcggtgaa caaaggtttt aattgccagt tgcgcgcgtt
     9901 cgtgaccttc atgccaggct ttttccagaa cacgtaaatc cgaagaaaga ccggaaatac
     9961 ctaataatcc cgactcttta ttcactacgc gttccaggtc acccaggctc tggttggttt
    10021 ggctggcgac ccaggacatc gcaccaaagt cgacatcgcc actgcgggta cccatcatca
    10081 agccttccag cggcgtcatt cccattgaag tatcaacact ctgaccgttg cgaaccgcgc
    10141 agattgacgc gccattgcca agatgcgcca caaccaggcc ggagtcatct tccgccagat
    10201 tcagcagcga atgtgcgcgc tgggaaacat agcggtgcga cgtgccgtgg aaaccataac
    10261 ggcgtacacc taactcttca taatatttcc acggcaggcc gtataaataa gcttccggag
    10321 ccatcgtctg gtggaaactg gtatcaaata ccgccacctg agttacgccc ggaaataatt
    10381 gctgcgccga ttcaatacca cttaaattgg cgtaattatg caggggtgcc agtggagaaa
    10441 cgcgacggat attatcaatg acttcatcgg taataatggc ggactcggta aaaatactgc
    10501 cgccgtgagc gatgcggtgg ccaattaagg ccacgctgtc atttaaattc cgtttttcca
    10561 gttcaaatgc aattgccttc aatgcacctt cgtagctgtg gtgagccagc ggtgctggct
    10621 ctcccccatt tacggataag aatgcatttt ccgagttaat accgtcggca atacctgaca
    10681 ttaatacttc acagtcgctg gcatcgagta cggaaaactt aatcgaagac gaaccacagt
    10741 taataaccaa aacaaccgga aattcattca tctcttttct catcctgagt tacggattaa
    10801 aacagtttgt atacgatgtt caggatggtc agcagaccaa tcacggtaac aaacacgtta
    10861 tccagacgac cacggtattt cgccagagac ggcgctttac ggatggcata catcggcaac
    10921 aggcacagca gggatgcgat aatcggtgcg cccatggctt caatcaggtc gaggatgttc
    10981 gggttggcgt aggcaacaac ccaggtggag cccatgatga agatcatgct gagagtattc
    11041 agtttaccca gcgacacttt ggttttgtca cctttataac cgaacttcag aatcagacca
    11101 ttcaagcctt ccagcgtccc cagatagtga ccgaagaaag atttgaagat agccacgagt
    11161 gcgatgatgg aagccgcata ttccagtgta atcgcgaacg ttgttttggt accggtcatg
    11221 gacgcaaagt ggttagccag ataagaaagc actggaatat tctgcgcttt ggcttccgcc
    11281 atgttggccg gagacagagt aaacaggcag ctaaaggcaa agaacatcac cactgcaacc
    11341 atcagcatgc tggcacgaga aatgatttgg gaacatttac gttcggtgaa gtcgcgaccg
    11401 aagtctttct catactcttc acgtttagaa accacgaagg aagagacgat tggcgagaag
    11461 ttaaaggaga aaaccatgat ggaaatcccc agccagacag tgatcaggat accgtcatga
    11521 ccggttaacg acagcgaacc gaggtcaacc tggtcgataa ctgcagagtt ccagtaaggg
    11581 atcagcgaca aagaaatcag caccaggctg gcgataaacg gccataccag gtagctcatc
    11641 actttaacca tcagatcctt accaaaccag atgacgaaag ccatcagcag caacaggaac
    11701 agcgccacaa agccgcgatt cagcggtgca aagccgagct ggttttccca gaacgtcata
    11761 aaggtattgg taatagtaac gccataaatc cacagcagtg ggcaaatcgc gaagaagtac
    11821 aggaacgtga taaccacgcc gccagtttta ccaaaatgct cttccaccgt ttccgtaatg
    11881 ttgccggaag ggttagagcc agaaagacac agacgcgcca gcgcccggtg gcaataaaac
    11941 gcgatggggt atgccaatac caacatcaga agaatcggga tcagtccgcc aaaacctgcg
    12001 cggataggga agaacagcac cccggcgccg attgccgtac caaacaagcc taacgtccat
    12061 gtggtatctg atttacgcca ggacgattgt tttgtctggc tggatacaat gctatctgaa
    12121 gtactcatat cctatcctca acgaattaat taagcgtcaa cgaaaccggt gatttgagag
    12181 acgcgagaaa gatcgatatt gccgccggaa ataatactga cggtttttct gttttgaata
    12241 tattggtcta atttaccgct taataatgca gcacatgcca gagcgcctgc gccttcggtg
    12301 acgactttat tgcgctgaat taaggcaatc atactgtttc tgatttcgtc ttcgctgacc
    12361 agcacgatgt catcgactaa ttcacgaacg atttcgtaag ttaaattacc cgggcgggag
    12421 acatcacaac catccgccag ggtgccggta gttcggtgcg tggttatttc tccggagtgg
    12481 aaagaagccg ccatgccgtg aacgttttca gactgtacgc caataacacg aatggtcggg
    12541 ttaatagatt taattgccac cgcaatacca gcaattaaac cgccaccacc aattggcaca
    12601 atcacgttat cgacatcata gagatcttcc ataatttcca gaccaatcgt tccctggcca
    12661 gcaatcactt tcggatcatc gtaaggtggg ataaaaatac ggccttccat ttcgacaatt
    12721 tcgctcactt tagcgatagt gtcgttgaag ttatcaccat gcagaacgac ttctgcggag
    12781 tagtcgcacg ttgccgctac tttggatttt ggcgcacctt ttggcatcac cactttaccg
    12841 tcgataccca gcatcgcgca ggagagggaa accccttgcg catggttgcc cgcagaacag
    12901 gccaccacgc ctttgcgttt ttccgcatcg gtcagtgaac ttaatttatt aaatgcgcca
    12961 cgaattttaa atgaacccgt acgctgcata ttttcaaact tcaggaatat ttcacctttg
    13021 caacgttcac taaaatagtt ggagcgaggc atgcctgttt tataaattcg cccagccagt
    13081 cgttgtttcg cttcaataat gtcatcaata gcaaccggca gatcgtatgt aatatgcatt
    13141 ataaaacctc ttcgcctgaa ttaaatatgt aggtaaccgt aaccgacacc tgcaagacag
    13201 acaggtggat tatttatgtt taaataaaaa caaataacta accaacttct attaattgcc
    13261 ttcgtctaca cccattataa gatgaatact ctttggctaa ttccaccaaa accgatgctg
    13321 cttttttaat acgataattt ttcgaccata ccgcggcata ttgtgccaca ggtaatgttt
    13381 cttcaaccgg aatagtaata aattgattag aaccaaaagg tgacgtcata tcacaaggaa
    13441 ttacagttaa gaaatcagca ttgagaacaa gattataaat tgtcacgact gagtcggttt
    13501 taacgatgtt ttcaatactg atgccatttc tttgtaacgt agtaagcagt tcgctgtagt
    13561 accccatatt agtttgtggc aacacccact gttcgttctt caacgactcc agcgtggtgg
    13621 tgccggtgca tgttcgggac ttactggcta ccagcacaaa ctcggactcg aacagcggct
    13681 caacatgtaa atcctgaagc ttcatttctg cacttaacgt accaatcgca aaatccagtc
    13741 gaccgtcgcg gattgccggt aagaacgaag acagttgcgc ttcatacata gaaacctgcg
    13801 ctttcgggaa cacctctttg aacttgttga tcatccctga cataaaagta aaaccaatca
    13861 atgaaggaaa accaaatgag acttccacca ccgcctcaga agacataccg cttatctcat
    13921 taaccatatt tttcatttca cgggtaatgg attcggaacg ggagagtaac aattgaccgg
    13981 caggtgttaa tgttacaccg gtatttttcc gcaccactaa ttccacacca aaataatctt
    14041 caatatcgtt aatgatttta ctgacggccg gttgagttaa ccctaattct tttgcagccg
    14101 agccgataga accacttcta atgacttcct gaaagactat caggtgctgc gttttcggaa
    14161 gaagaatagt gctcataacg acctacgtta attacctcat tgacggcatg aagtgtatca
    14221 aaatgaaatg aacaggatat gtgcgaccac tcacaaatta actttcaata ctttccagag
    14281 tatcgtta
//
LOCUS       DAOFQD010000118        14218 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.118, whole genome shotgun sequence.
ACCESSION   DAOFQD010000118 DAOFQD010000000
VERSION     DAOFQD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.118"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(66..554)
                     /gene="eco"
                     /locus_tag="QBF46_003700"
     CDS             complement(66..554)
                     /gene="eco"
                     /locus_tag="QBF46_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDQ6675832.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            962..1456
                     /gene="napF"
                     /locus_tag="QBF46_003701"
     CDS             962..1456
                     /gene="napF"
                     /locus_tag="QBF46_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDQ6675833.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            1446..1709
                     /gene="napD"
                     /locus_tag="QBF46_003702"
     CDS             1446..1709
                     /gene="napD"
                     /locus_tag="QBF46_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDQ6675834.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1706..4192
                     /gene="napA"
                     /locus_tag="QBF46_003703"
                     /pseudo
     CDS             1706..4192
                     /gene="napA"
                     /locus_tag="QBF46_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
     gene            4199..4894
                     /gene="napG"
                     /locus_tag="QBF46_003704"
     CDS             4199..4894
                     /gene="napG"
                     /locus_tag="QBF46_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDQ6675835.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4881..5744
                     /gene="napH"
                     /locus_tag="QBF46_003705"
     CDS             4881..5744
                     /gene="napH"
                     /locus_tag="QBF46_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDQ6675836.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5741..6190
                     /gene="napB"
                     /locus_tag="QBF46_003706"
     CDS             5741..6190
                     /gene="napB"
                     /locus_tag="QBF46_003706"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDQ6675837.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            6200..6802
                     /gene="napC"
                     /locus_tag="QBF46_003707"
     CDS             6200..6802
                     /gene="napC"
                     /locus_tag="QBF46_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDQ6675838.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6821..7438
                     /gene="ccmA"
                     /locus_tag="QBF46_003708"
     CDS             6821..7438
                     /gene="ccmA"
                     /locus_tag="QBF46_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDQ6675839.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            7435..8004
                     /locus_tag="QBF46_003709"
     CDS             7435..8004
                     /locus_tag="QBF46_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDQ6675840.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMALTL
                     LLGTPTLGFLGAPGVALTVGLKRGGVLLSILVLPLTIPLLIFATAAMDAASMHLPVDG
                     YLAILGALLAGTATLSPFATAAALRISIQ"
     gene            8046..8783
                     /gene="ccmC"
                     /locus_tag="QBF46_003710"
     CDS             8046..8783
                     /gene="ccmC"
                     /locus_tag="QBF46_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDQ6675841.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8780..8989
                     /gene="ccmD"
                     /locus_tag="QBF46_003711"
     CDS             8780..8989
                     /gene="ccmD"
                     /locus_tag="QBF46_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDQ6675842.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8986..9465
                     /gene="ccmE"
                     /locus_tag="QBF46_003712"
     CDS             8986..9465
                     /gene="ccmE"
                     /locus_tag="QBF46_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDQ6675843.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            9462..11405
                     /gene="ccmF"
                     /locus_tag="QBF46_003713"
     CDS             9462..11405
                     /gene="ccmF"
                     /locus_tag="QBF46_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDQ6675844.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            11402..11959
                     /gene="dsbE"
                     /locus_tag="QBF46_003714"
     CDS             11402..11959
                     /gene="dsbE"
                     /locus_tag="QBF46_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDQ6675845.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11956..13008
                     /gene="ccmH"
                     /locus_tag="QBF46_003715"
     CDS             11956..13008
                     /gene="ccmH"
                     /locus_tag="QBF46_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDQ6675846.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKTDPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(13043..13690)
                     /gene="narP"
                     /locus_tag="QBF46_003716"
     CDS             complement(13043..13690)
                     /gene="narP"
                     /locus_tag="QBF46_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDQ6675847.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            14010..>14218
                     /locus_tag="QBF46_003717"
     CDS             14010..>14218
                     /locus_tag="QBF46_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675848.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKE"
BASE COUNT         3042 a         3597 c         4110 g         3469 t
ORIGIN      
        1 cgttggatgt cggatgcggc gtgaccgcct tgtctgacct acggggtgcc gcacttcacg
       61 gcagtttagc gaactaccgc gttgtcaatt ttctcttccg ccttccagac gcggtacttc
      121 acatctacat tgtctggcgt atacaccacg atcggcagct tgctgttgta acgcagcatt
      181 ccagcatcgc ccagatacgc ggtgacaaat ttcttctctt tcttgccatc cgggcaggcc
      241 atcatcgtcg aaaccgggga actgacttta tcaaaaacat aataatcata gccccagcct
      301 tccagcgttt tgttttccag cttcccgccg agacgatgca aattgcaatc gacttccagc
      361 gtctgaccga ttaacagttc tactttcagg gtagattcat cttcttgcgg ggttaactga
      421 atcacctgac gcttcatccc tttttcagct tgtggataag gcgcgatttt ttccagtggc
      481 tggacgcttt ctgccgccca ggcggaagtg gtagcgaaag cagcaaacaa tactgcaggt
      541 agaatggtct tcattttttt gctttccttc ttcatttata ggttttcacc gatgatgtcg
      601 ctgaaggtta acatattcat cagatttatt aatttgcttt atgaataact taaagaacat
      661 tcagacaatt ttacctgctc ttttagtgtt aaattctaat gagagagcaa accagcagaa
      721 atatctctgg tgaattaatc gctatataaa tatatttata atcatttgaa atgtgagcaa
      781 aagcccgttt ttgccacgct tcccgccact cttttgatcc tgctacaggt tttaccccga
      841 tcggggtatg catctttgac acatccttta atatcttagc ggctataaaa atggcttatt
      901 aattatgcgg tttatttggt cgctctcaat tttcagagcg cgttaatgat ggaaggtcaa
      961 tgtgaagatt gatgcatccc gtcggggcat actcactggt cgctggcgca aagccagtaa
     1021 cggtatccgt ccgccctggt ccggtgatga atctcatttt ctgacccatt gcacccgctg
     1081 cgacgcctgt attaatgctt gtgaaaacaa cattctgcaa cgcggcgcgg gcggctatcc
     1141 gagcgttaat ttcaaaaata acgagtgcag cttctgttac gcctgcgcgc aggcttgccc
     1201 cgaatcgcta ttttctccgc gccacaccag ggcctgggat ttgcagttca ccattgggga
     1261 cgcgtgcctg gcgtatcagt ctgttgaatg ccgccgctgt caggatagct gtgagccaat
     1321 ggcaattatc tttcgcccga cgctctccgg gatctaccag ccgcaactta atagccaact
     1381 ttgcaacgga tgtggagcat gtgcagccag ctgtccggta tcagccataa ctgcggagta
     1441 tctccatgca cactaactgg caagtttgca gcctggtcgt gcaggccaaa agcgaacgaa
     1501 tttcagacat cagcacccaa ctgaacgcct ttcccggctg tgaagttgct gtcagcgacg
     1561 cgccgagcgg tcagttgatt gtggtggtgg aagcagaaga cagcgaaacg ctgatccaaa
     1621 ccattgagtc agtacgcaac gtagagggcg tgctggcggt gtcgctggtt tatcaccagc
     1681 aggaagagca aggtgaggaa acaccatgaa actcagtcgt cgtagcttta tgaaagctaa
     1741 cgccgttgcg gccgctgcgg cggctgccgg tctcagcgtg ccgggcgttg cccgcgccgt
     1801 tgttggtcag caggaagcca tcaaatggga taaagcgccg tgccgtttct gcggtactgg
     1861 ttgcggcgtt ctggtcggaa cgcagcaggg acgtgtggtg gcctgtcagg gcgacccgga
     1921 cgcaccggtt aaccgtggcc tgaactgcat taagggctat ttcctgccca aaatcatgta
     1981 cggtaaagac cgtttgacgc agccgctgct gcgtatgaaa aacggtaaat atgacaaaga
     2041 aggcgaattt accccaatca cctgggatca ggctttcgat gtgatggaag agaaattcaa
     2101 aaccgccctg aaagaaaaag ggccggaata gatcggtatg ttcggttctg gtcagtggac
     2161 tatctgggaa ggttatgccg cgtccaagct gtttaaagcg ggcttccgtt cgaacaacat
     2221 cgacccgaac gcacgtcact gtatggcgtc ggcagtagtt ggctttatgc gtacctttgg
     2281 tatggatgag ccgatgggct gctatgacga catcgagcag gctgacgcgt ttgtgctgtg
     2341 gggcgctaac atggcggaga tgcacccgat cctctggtca cgcatcacta accgtcgtct
     2401 ctctaaccag aacgtcaccg tggcggtgct ttctacctac cagcatcgta gcttcgagct
     2461 ggcggataac ggcatcatct ttacgccgca atctgacttg gtgatcctga actacatcgc
     2521 caactatatc attcaaaaca atgcgataaa tcaggacttc ttcagcaaac acgttaacct
     2581 gcgcaaaggg gcgacggaca tcggctacgg tttacgtccg acccatccgc tggaaaaagc
     2641 agcgaagaat ccaggttctg acgcttccga accgatgagc tttgaagatt acaaagcctt
     2701 cgttgctgag tatacgctgg aaaaaaccgc tgaaatgacc ggcgtgccga aagaccagtt
     2761 agaacaactg gcgcagctgt atgccgatcc gaacaagaaa gtcatctcct actggacgat
     2821 gggcttcaac cagcataccc gtggtgtgtg ggccaacaac ctggtctaca acctgcacct
     2881 gctgaccggc aaaatttccc agccgggttg cggtccgttc tccctgaccg ggcagccttc
     2941 cgcgtgtggt actgcgcgtg aagtgggcac ctttgctcac cgtctgcctg cggacatggt
     3001 ggtgactaac gagaaacatc gtgatatctg cgagaagaag tggaatatcc cgagcggcac
     3061 cattccggcg aaaatcggtc tgcatgcggt agcacaagac cgtgcgctga aagacggcaa
     3121 gctgaatgtt tactggacca tgtgtaccaa caacatgcag gccgggccga acatcaatga
     3181 agagcgtatg ccgggctggc gcgatccgcg caacttcatc atcgtctccg atccgtatcc
     3241 gacagtcagt gcgctggccg ccgacttgat cctgccgacc gcaatgtggg tagagaaaga
     3301 gggcgcttac ggtaacgccg aacgccgtac tcagttctgg cgtcagcagg tacaggcacc
     3361 gggcgaagcg aaatcggatc tctggcagtt agtccagttc tcccgccgct tcaaaactga
     3421 agaagtatgg ccggaagagc tgctggcgaa gaaaccggaa ctgcgtggca aaacgctgta
     3481 cgaagttctg tatgccaccc ccgaagtgag caaattcccg gtatccgaac tggcggaaga
     3541 tcagctgaac gatgaatccc gcgagctggg cttctatctg caaaaagggc tgttcgaaga
     3601 gtacgcatgg tttggtcgcg gtcacggtca cgatctggca ccgttcgatg actaccacaa
     3661 agcgcgcggt ctgcgctggc cggtggtgaa cggtaaagaa acgcagtggc gttacagcga
     3721 aggtaacgac ccgtacgtga aagcgggcga aggctacaag ttctacggta aaccggatgg
     3781 caaagcggtg atcttcgcgc tgccgttcga accggcggcg gaagcaccgg atgaagagta
     3841 cgacctgtgg ctctctaccg gacgcgtcct ggagcactgg cacaccggca gtatgactcg
     3901 ccgtgtgccg gaactgcacc gcgccttccc ggaagcggtc ctgtttattc acccgctgga
     3961 tgcgaaagcg cgcgatctgc gccgtggcga caaagtgaaa gtggtttctc gccgtggcga
     4021 agtgatctcg attgttgaaa cgcgcggtcg taaccgtccg ccacagggcc tggtgtacat
     4081 gccgttcttc gacgccgcac agctggttaa caaactgacg ctggatgcga ccgatccgct
     4141 ctcgaaagag acggacttca agaagtgcgc ggtcaaactg gagaaggtgt aagccattat
     4201 gtcccggtca gcgaaacctc aaaatggtcg ccgccgcttt ctgcgcgatg ttgttcgcac
     4261 agcaggcggg ctggctgccg tgggtgtggc gctggggtta caacagcaaa ccgcacgcgc
     4321 atctggcgtg cggttgcgcc cgcccggagc cataaacgag aacgcctttg ccagtgcctg
     4381 tgtgcgttgc ggtcagtgtg ttcaggcttg cccttacgac accttaaaac tggcgacgct
     4441 ggcctctggt ctgtcggcgg gcacgccata ttttgtcgca cgggatattc cttgcgaaat
     4501 gtgtgaggac attccgtgcg ccaaagtgtg cccgagcggt gcgctggatc gtgagatcga
     4561 atcgatcgac gacgcgcgga tggggctggc ggtactggtg gaccaggaaa actgtctcaa
     4621 ctttcagggg ctgcgctgcg atgtttgtta tcgcgaatgc ccgaaaattg atgaggccat
     4681 caccctggag ctggagcgca acacgcgtac cggtaagcac gcccgctttc tgccgacggt
     4741 tcacagcgac gcctgtaccg gttgcggtaa gtgcgaaaaa gtatgcgtgc tggaacaacc
     4801 ggcaatcaag gtgttaccgc tgtcactggc gaaaggggag ttaggtcacc attaccgctt
     4861 cggctggctg gaggggaaca atggcaaatc gtaaacgtga cgccgggcgc gaggcgctgg
     4921 agaaaaaagg ctggtggcgc agtcaccgtt ggctggtgtt acgtcgcctt tgtcagttct
     4981 tcgtgctggg gatgtttttg agcggtccat ggtttggtgt gtggatcttg cacggcaact
     5041 acagcagtag cctgttattc gacaccgttc cgctgaccga tccgctgatg acgctgcaaa
     5101 gtcttgccag tggtcatctg cctgccacgg tggcgttgac cggtgcggtg attatcaccg
     5161 tgctctacgc cctggcggga aagcgattat tttgcagctg ggtctgcccg ctgaacccga
     5221 ttaccgactt agcgaactgg ttacgcaggc gctttgacct caatcagtct gcgacgatcc
     5281 ctcgccacat tcgctacgtg ctgttggtgg tcattctggt gggatcagcc ttaactggca
     5341 cgctcatttg ggaatggatc aaccccgttt ctctgatggg gcgtagcctg gtaatgggct
     5401 tcggcagcgg cgcgctgctt attctcgcgc tgtttttatt tgatctactg gtcgttgaac
     5461 acggctggtg cgggcacatt tgtccggttg gcgcgctgta tggcgtgctg ggtagcaaag
     5521 gtgtgattac cgttgcggcc accgatcggc agaaatgtaa ccgctgtatg gattgttttc
     5581 atgtttgccc ggaaccgcat gtgctacgtg ccccggtgct ggatgaacaa agcccggtgc
     5641 aggtcaccag ccgcgattgc atgacgtgcg gtcgctgcgt ggatgtctgt tctgaggatg
     5701 tatttacaat aactacacga tggagttcgg gagcgaaatc atgaaaagcc atgacctgaa
     5761 gaaagcgctg tgtcaatgga cggcgatgct ggccctggtg gtaagcggcg cggtttgggc
     5821 ggctaatggc gtcgatttta gccagtcgcc ggaagtatcc gggacacagg aaggggccat
     5881 tcggatgcca aaagagcagg aacggatgcc gctgaactat gtgaatcagc cgccaatgat
     5941 cccgcatagc gttgaaggtt atcaggtaac gaccaatacc aaccgctgct tgcagtgcca
     6001 cggtgtcgaa agctatcgca ccactggcgc gccgcgtatc agtcctactc actttatgga
     6061 cagcgacggc aaagtgggcg cggaagtggc tccgcgtcgt tatttctgtc tgcaatgtca
     6121 cgtaccgcag gccgataccg cgccaatcgt ggggaatacc tttaccccat caaaaggtta
     6181 cgggaaataa gaggttatta tgggaaattc tgaccgtaag cctggtctga ttaagcgcct
     6241 gtggaaatgg tggcgtaccc ccagccgtct ggcgctgggg acgctgctgt tgatcggttt
     6301 tgttggcggc atcgtcttct ggggtggctt taacaccggg atggaaaaag ccaataccga
     6361 agagttctgc attagctgcc acgaaatgcg caacacggtg tatcaggaat acatggattc
     6421 cgtgcactac aacaaccgta gcggcgtccg tgcgacctgt ccggattgtc acgttccgca
     6481 cgagtttgtg ccgaagatga tacgcaagct caaagcaagt aaagagctgt atggtaaaat
     6541 ttttggcgtt attgacacgc cgcagaaatt tgaagctcat cgtctgacga tggcacagaa
     6601 tgagtggcgg cgcatgaagg acaataactc gcaggagtgc cgtaactgtc acaacttcga
     6661 gtatatggat acaaccgccc agaaatcggt tgccgcgaag atgcatgacc aggcggtgaa
     6721 agatgggcaa acctgtattg attgccataa agggatagcg cacaagctgc ccgatatgcg
     6781 tgaagtcgag ccaggttttt aacagggtta ttgcgtgggt atgcttgaag ccagagagtt
     6841 actttgtgag cgggatgaac gaaccttatt tagtggcttg tcatttacgc tgaacgcagg
     6901 agagtgggta caaatcaccg gtagcaacgg cgcggggaag acaacgcttc tccgtttgct
     6961 gacggggttg tctcgccctg acgcaggcga ggttctctgg caagggcagc ccttgcatca
     7021 ggtacgcgac agctaccatc aaaacctgtt atggataggc catcagccgg ggatcaaaac
     7081 ccggctgacg gcgttagaaa atctgcactt ttatcatcgc gatggcgata ccgcacaatg
     7141 tctggaagcc ctggcgcagg ccgggcttgc cggattcgaa gatattcctg taaatcagct
     7201 ctcggccggg caacaacgcc gcgtcgcttt agcgcgtctg tggctgaccc gtgccacgtt
     7261 atggatcctc gacgagcctt ttaccgcgat tgacgttaac ggcgtcgatc gtctgaccca
     7321 gcgtatggcg cagcatacgg agcagggggg gattgtgatt ctgactaccc accagccgct
     7381 caacgttgct gaaagtaaaa ttcgccgcat ttcactgacg caaacgaggg ccgcatgatg
     7441 ttctggcgca ttttccgtct tgagctgcgt gtagcgtttc gccatagcgc cgaaatcgcc
     7501 aacccgctgt ggttcttcct gattgtaatt accctttttc cgctcagtat cggtccggag
     7561 ccgcaactgc tggcgcgtat tgcaccgggc attatctggg ttgctgcgct gctttcatcc
     7621 ttgctggcgc tggaacgact gttccgtgac gatttgcagg acggcagtct tgaacaattg
     7681 atgttgttgc cgttaccctt gcccgccgtt gtgctggcga aggtgatggc gctgacgctg
     7741 ctgctgggaa cgcctacgct tggctttctc ggtgcaccgg gtgtggcgtt gacagtggga
     7801 cttaagcgcg gtggtgtgct gctcagcata ctggtgttac cgctgactat cccattactc
     7861 atctttgcca ccgccgcgat ggacgcggct tccatgcatt tgcccgttga cgggtatctg
     7921 gcaattttag gcgcgttgct ggcaggcacc gcgacattaa gtccttttgc gacggcggca
     7981 gcgttacgaa tcagcattca ataacgctgg attactgatt tttgttcgag tctggtatcg
     8041 aaactatgtg gaaaacactg catcaactgg cgatcccacc acggctgtat caaatctgtg
     8101 gctggtttat accgtggctg gcaattgcca gtgtggtcgt gcttaccgtc ggctggatct
     8161 ggggattcgg ctttgctccg gctgattatc agcagggaaa tagctaccgc attatctacc
     8221 tgcatgtgcc tgcggcgatc tggtcgatgg gcatttatgc atcaatggca gtggcagcgt
     8281 ttattggcct tgtctggcag atgaaaatgg ccaacctggc ggtggcggcg atggccccca
     8341 ttggtgccgt gtttaccttt attgccctgg ttaccggctc tgcatgggga aaaccgatgt
     8401 ggggcacctg gtgggtatgg gatgcacgtc tgacttctga actggtgctg ctgtttttgt
     8461 atgtgggtgt gattgccctg tggcacgcct tcgacgaccg ccgtctggcg ggccgtgcgg
     8521 caggtatcct ggtgctgatt ggcgtggtga atctgccgat tattcattac tccgtggagt
     8581 ggtggaacac cctgcatcag ggatcaacgc ggatgcagca aagtatcgat ccggcgatgc
     8641 gttcgccgct gcgctggtcg atttttggct tcctgctcct gtctgccacg ctgacgctga
     8701 tgcggatgcg taatttgatt ttgctgatgg aaaaacgccg tccgtgggtg agtgaactga
     8761 tactgaaaag aggccgtaaa tgacccctgc atttgcttcc tggaatgaat ttttcgcaat
     8821 gggcggttac gccttttttg tctggctggc ggtggtgatg accgttattc cgctggtggt
     8881 tttggtcgtg cactcggtga tgcaacatcg cgcaattctg cgtggtgtgg cgcaacagcg
     8941 ggcgcgtgag gcgcgtttac gtgctgcgca acagcaggag gctgcatgaa tattcgccgt
     9001 aaaaaccgct tgtggattgc ctgtgccgtg ttggcagggc tggcgctgac tatcggtctg
     9061 gtgctatatg cgctgcgctc gaatatcgat ctcttttata cgccggggga aattctctac
     9121 ggcaagcgtg aaactcagca aatgccggaa gtcggtcagc gtctgcgcgt tggcgggatg
     9181 gtgatgccgg gtagcgtgca gcgcgatccc aattcgctaa aagtaacttt caccatttac
     9241 gacgctgaag gttcagtaga tgtctcttac gaaggcattt tgccggatct gttccgtgaa
     9301 gggcagggcg ttgtggtgca gggcgaactg gaaaaaggca atcatatcct cgcaaaagaa
     9361 gtgctggcga aacacgacga aaactacacg ccgccagaag ttgagaaagc gatggaagcc
     9421 aatcaccgtc gcccggcgag tgtttataag gacccagcat catgatgccg gaaattggta
     9481 acgggctgct gtgtctggcg ctaggaattg cgctgctgct gtccgtgtat ccgctatggg
     9541 gcgtagcgcg cggagatgcg cgcatgatgg cgtcttcccg cttgtttgcc tggctgctgt
     9601 ttatgtctgt ggctggcgca tttctggtac tggtcaatgc cttcgtggtc aacgacttca
     9661 ccgtcaccta tgttgccagc aactccaata cccagcttcc ggtgtggtat cgcgtggcgg
     9721 ctacctgggg cgcgcatgaa ggctcgctcc tgctgtgggt gctgctgatg agcggctgga
     9781 cctttgcggt agcgattttt agtcagcgta ttccgctgga tattgtggcc cgcgtactgg
     9841 cgataatggg gatggtcagc gtcggctttt tgctgttcat tctctttacc tctaacccgt
     9901 tctcacgcac gttgccgaac ttcccgattg aagggcgcga tcttaacccg ctgttacagg
     9961 atccggggct gatcttccat ccgcctctgc tctatatggg gtacgtgggg ttctcggtgg
    10021 cgtttgcttt tgccattgct tctttgctga gcgggcgtct ggacagcact tatgcgcgtt
    10081 ttactcgccc gtggacgctg gcggcgtgga ttttcctgac gctcggcatt gtgctcggtt
    10141 ccgcatgggc ctattacgaa ctcggctggg gcggctggtg gttctgggat ccggtagaaa
    10201 acgcctcgtt tatgccgtgg ctggtgggga ctgcgctgat gcactcactg gcggtcactg
    10261 aacaacgcgc cagtttcaaa gcgtggacat tactgctggc aatcagtgcc ttctcgttgt
    10321 gtctgctggg gaccttcctc gtgcgttccg gcgtgctggt atcggtacac gcgtttgcgt
    10381 ctgatccggc gcgcggtatg tttatcctcg cctttatggt gctggtgatt ggcggttcgc
    10441 tgctgctgtt tgccgcgcgt ggacacaaag ttcgctcacg cgtaaacaat gcgctgtggt
    10501 cgcgggaatc tttgctgtta gcgaacaatg ttttgctggt cgctgcgatg ctggtggtgt
    10561 tgctggggac gctgctgccg ttggtgcata agcaactggg actgggcagt atttcgattg
    10621 gcgaaccgtt cttcaacacc atgtttacct ggctgatggt gccgtttgcg ctactgcttg
    10681 gtgtcggtcc tctggtgcgc tgggggcggg atcgcccgcg taagatccgc aatttattga
    10741 ttatcgcctt catctctacg ctggtgctgt cgctgctgtt gccgtggctg ttcgaaagca
    10801 aagttgtggc gatgacggtg ctcggcctgg caatggcctg ctggattgcg gtgctggcaa
    10861 ttgcggaagc tgcgctacgt atttcacgcg gcacgaaaac caccttcagt tattggggga
    10921 tggtggcggc tcaccttggg ctggcagtga caattgttgg cattgccttt agccagaact
    10981 atagcgttga gcgtgatgtg cgcatgaagt ccggcgatag cgtcgatatt catgaatatc
    11041 gcttcacctt ccgtgatgtc aaagaggtga ctggcccgaa ctggcgtggc ggtgtggcga
    11101 ctatcggcgt aacgcgcgat ggcaagccgg aaacggtgct gtatgcggaa aaacgttatt
    11161 acaacactgc cgggtcgatg atgaccgaag cggcaattga cggcggcatc acgcgtgacc
    11221 tgtacgccgc gctcggtgaa gagctggaaa acggcgcgtg ggccgtgcgt ctttactaca
    11281 aaccatttgt tcgctggatt tgggcgggcg ggctgatgat ggcgttgggc ggactgctgt
    11341 gtctgtttga tcctcgctat cgtaagcgcg tgagtccgca aaaaactgcg ccggaggccg
    11401 tatgaagcgc aaagtattgt taattccgtt gattatcttc ctggcgattg ccgcggcgct
    11461 gctgtggcag ctggcgcgta atgccgaagg ggatgatccg accaatctgg aatcggcgct
    11521 cattggcaag cctgtgccga agtttcgtct cgaatcactg gacaatccgg ggcagtttta
    11581 tcaggcggat gtgctgactc agggcaaacc agtactgctt aacgtctggg cgacctggtg
    11641 tccgacctgc cgtgcggaac atcaatatct gaatcagctt tcagcgcagg gcattcgcgt
    11701 ggtcggcatg aactataaag acgatcgcca gaaggcaatc agctggctga aagagctggg
    11761 caatccttac gcgctaagcc tgtttgatgg cgacggcatg ttagggctgg atctcggtgt
    11821 ctatggcgcg ccagaaacct tccttattga cggcaacggt atcattcgct atcgccatgc
    11881 gggcgatctc aatcctcgcg tctgggaaga agagatcaag ccgctgtggg agaaatacag
    11941 taaggaggcc gcacaatgag gtttttattg ggcgtgctga tgctgatgat ctccggctca
    12001 gcactggcga ccatcgacgt gttgcagttt aaagatgaag cacaggaaca acagttccgt
    12061 cagctcactg aagaactgcg ctgcccgaaa tgccagaaca acagcattgc cgattccaac
    12121 tcgatgattg ccaccgacct gcgtcagaaa gtgtatgaac tgatgcagga aggtaaaagt
    12181 aaaaaagaga ttgtcgatta tatggtggcg cgttacggca acttcgtcac ttacgatccg
    12241 ccgttaacgc cgctgaccgt gctgctgtgg gtgcttccgg tagtggctat tggcattggc
    12301 ggttgggtca tatacgcccg ctcgcggcgt cgggtacgcg tagtgccgga agcgtttcct
    12361 gaacaaagcg tgcaggaagg taagcgtgcc ggatatattg tttatctgcc gggtattgtg
    12421 gtggcgttaa ttgtggctgg cgtcagctac taccagactg gcaattatca gcaggtgaaa
    12481 atctggcagc aggccacggc acaggctccg gcgttgctgg acagggcgct ggatccgaaa
    12541 accgatccgc tcaacgaaga agagatgtcg cgtcttgcgc tggggatgcg tactcaactg
    12601 caaaaaaatc cgggagatat agaaggctgg attatgttgg gccgcgttgg catggcgctg
    12661 ggtaacgcta gtatcgccac cgatgcatac gctactgcgt atcgcctcga tccgaaaaac
    12721 agcgatgccg cactgggtta tgctgaagcg ttgacacgtt catctgatcc caacgacaac
    12781 cgcctcggtg gtgaactgct gcgccagttg gtgagaagcg accacagcaa tatccgtgtg
    12841 ttaagcatgt atgcgtttaa tgcctttgag cagcagcgat ttggcgaagc cgttgccgcg
    12901 tgggagatga tgttgaaact cttacctgcc aacgatactc gccgtgcggt gattgaacgt
    12961 agtatcgcgc aggcgatgca acatttgtcg ccgcaggaga gtaaataaaa agaggcccga
    13021 tggtgatgcc atcgggctat ttttattgtg ccccgcgttg ttgcaggaac agaatggtgg
    13081 ctgccacgcg tgagcggaca ttgagtttgc gcagcagatt gcgaatatgt acttttactg
    13141 tctgctctga aatattcaac accgaggcaa tctgtttatt tgacagcccc tgtgccagct
    13201 cgtgcagaac atccagctcg cgctccgtca gcacgctgaa gggatcttct tccgcgccaa
    13261 acatttcacg ttcacgtaag tactgattga cgcgttcgct aaagactttg ctgcctttcg
    13321 ctccagcacg aattgcttcc agcaatactt ccgggtcgct gtctttcaac agataaccgt
    13381 ctgcgcctgc gtctatcagc gcaaagacat cgctggaggc atctgatacg gtcaggataa
    13441 taatttgcgc ggtaacgcca tccctgcgca aggcattgag ggtatccagg ccactcatac
    13501 ctttcatatt gagatccagc aagatcacgt cgatatccag tctattcgcc agatcgatcg
    13561 cgctcgcgcc gtcgcccgct tcggcgacca cttcaaagcc aggatcaagc tccagtaact
    13621 gacgaacacc gcgtcgcata agtggatgat catccacaat catcacctga aaaggcgttg
    13681 cttcaggcat agtagtctcc tgaggtttta ttagaatcat tatgtttgag cgtagcgttg
    13741 gtaacgctac caggcgcgtg aatgttaccc cattttatgt atggacatgt agtgaaaact
    13801 acaaccttgt cgttcgcttt ggcatcattt tcattataaa gacggaattt tgcatcagct
    13861 tctcatcaag aaaaacaact aaaaaatcaa tcacaatact tgtgcatttc tttaaacagt
    13921 ttttaaaaac tgactccggg tatggagcta tgggtatttt ccgtacccaa tgcttttaac
    13981 agcaattaat ttcataggat gaaagctcaa tgcatcaatc tggttctgtt tctctttgtc
    14041 gttccgcaat atctgttctg gtggctacag cgttatattc acccatagca ttggcatcaa
    14101 cagttgagta tggtgagaca gttgatggtg ttgtcctgga aaaagatatc cagctggttt
    14161 atgggaccgc caataatacg aaaatcaatc ctggcggaga acagcatata aaagaatt
//
LOCUS       DAOFQD010000119        14110 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.119, whole genome shotgun sequence.
ACCESSION   DAOFQD010000119 DAOFQD010000000
VERSION     DAOFQD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.119"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            64..354
                     /locus_tag="QBF46_003718"
                     /pseudo
     CDS             64..354
                     /locus_tag="QBF46_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            378..1713
                     /locus_tag="QBF46_003719"
                     /pseudo
     CDS             378..1713
                     /locus_tag="QBF46_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            2045..3433
                     /gene="narU"
                     /locus_tag="QBF46_003720"
     CDS             2045..3433
                     /gene="narU"
                     /locus_tag="QBF46_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDQ6675849.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            3515..7255
                     /gene="narZ"
                     /locus_tag="QBF46_003721"
     CDS             3515..7255
                     /gene="narZ"
                     /locus_tag="QBF46_003721"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDQ6675850.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGSIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNNPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            7252..8796
                     /gene="narH"
                     /locus_tag="QBF46_003722"
     CDS             7252..8796
                     /gene="narH"
                     /locus_tag="QBF46_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDQ6675851.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAALRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            8796..9491
                     /gene="narW"
                     /locus_tag="QBF46_003723"
     CDS             8796..9491
                     /gene="narW"
                     /locus_tag="QBF46_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDQ6675852.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            9488..10168
                     /gene="narI"
                     /locus_tag="QBF46_003724"
     CDS             9488..10168
                     /gene="narI"
                     /locus_tag="QBF46_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDQ6675853.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            10247..11140
                     /locus_tag="QBF46_003725"
     CDS             10247..11140
                     /locus_tag="QBF46_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDQ6675854.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(11236..12081)
                     /gene="nhoA"
                     /locus_tag="QBF46_003726"
     CDS             complement(11236..12081)
                     /gene="nhoA"
                     /locus_tag="QBF46_003726"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDQ6675855.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDNQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVPDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            12254..12823
                     /locus_tag="QBF46_003727"
     CDS             12254..12823
                     /locus_tag="QBF46_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDQ6675856.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEHNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(12820..13053)
                     /gene="pptA"
                     /locus_tag="QBF46_003728"
     CDS             complement(12820..13053)
                     /gene="pptA"
                     /locus_tag="QBF46_003728"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDQ6675857.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSMNA"
     gene            complement(13153..>14110)
                     /locus_tag="QBF46_003729"
     CDS             complement(13153..>14110)
                     /locus_tag="QBF46_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc5 family transposase"
                     /protein_id="HDQ6675858.1"
                     /translation="YGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKD
                     VIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLILGQIKTDEKSNEITAIPELLNML
                     DIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPAH
                     DSYAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKELE
                     MTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGI
                     RHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLTGSGLS"
BASE COUNT         3531 a         3525 c         3470 g         3584 t
ORIGIN      
        1 aatgacgccg tttaaaattc gtttagaaaa tacgtttaac gataattaaa aggattctgt
       61 taaatgaaat taaaaatagt tgcggtggtt gtaactggtt tgttagctgc gaacgtagca
      121 cacgctgccg aagtctataa caaggatggt aataaactcg acctttatgg caaggttacc
      181 gctctacgtt attttactga tgataagcgt gacgatggtg ataaaactta tgcccgtctc
      241 ggctttaaat gagaaacgca aatcaatgat caaatgattg gttttggtca ctgggaatat
      301 gattttaaag gctataacga tgaagccaac tgctcgcgcg gcaagaatct ctgattgagt
      361 taagtgccca tcacaatatg atcactgacc ttatattaca caatcatccc aggatgaaaa
      421 caatcacttt aaacgacaac catattgcac atttaaacgc caaaaacact acaaaactgg
      481 aatatttaaa cttaagcaat aacaatttac tgccaaccaa tgacattgaa caactaatat
      541 catcaaaaca tctttggcat gtattagtta acggcatcaa caatgatcca cttgcacaaa
      601 tgcagtactg gactgcagta agaaatataa ttgatgacac taatgaagtg accattgatt
      661 tatcaggact taatttaacc actcaaccac cagggctgca aaacttcacc tctatcaatc
      721 ttgataataa ccaactcaca cattttgatg caaccaacta cgatagactc gtaaagctaa
      781 gtctgaatag taatgctctt gagtcaataa attttcctca aggcagaaat gtaagtatta
      841 cacatatatc tatgaataat aatgctctca gaaatattga tatagatagg ctttcatcag
      901 ttacttattt tagtgcggca cataatcaac tagagtttgt gcaattagaa tcttgcgaat
      961 ggctgcaata cctgaatctc agccataatc aattaactga tattgttgca ggtaataaaa
     1021 atgaactctt actgctggat ctatcccata ataaactaac aagtttacac aatgacttat
     1081 ttcccaactt gaatacgtta cttattaaca acaatttgct ttctgaaatt aaaatattct
     1141 atagcaactt ctgcaatgtt cagacattaa acgctgctaa caaccagttg aaatatataa
     1201 atcttgattt cctgacttat cttccatcta tcaaaagttt aagactggac aataataaaa
     1261 taacccacat tgatactaat aatacatccg atattggaac tttattcccc ataataaaac
     1321 agagcaaaaa cttaaatttt ttaaatgttt ctgggaagaa caattgccct actatgcagc
     1381 tcatgttatt taatttgttt tccccagcac ttaagcttaa tactggcctg gcaattcttt
     1441 cgcctggtgc atttgaagtt cactctgacg gaatagatgt ggataacgaa ttgtttcact
     1501 atcctattaa aaatagcata taccccatat aatatacaca cttacaagac agaggaagtt
     1561 gtaaaccaga ggaatataaa agttaaaaac atgtccttag atgaaataaa caatacttac
     1621 tgtaataacg attattacaa tcaggcaata agagaggaac cgatagacct tctggacaga
     1681 tcgttttcct ccagctcatg gccttttgag tgaataaatt attgccaatt ctataagtgt
     1741 cttgtcttgt cttgtcttgt taagaatata gttaatatac aaccctttgg attcatcatc
     1801 tgaagggttg aatcatgaca tgcgtacata gcttaacacc cgtttaatgg ttagcatgtt
     1861 agttacattt agtaacacat atctggctac ttaatcccca ttctgccccc gcggttacgc
     1921 tttcttatct attctttcaa tcagcacttc acccaattca tccttaattc taataactgg
     1981 ctattgcgcc ttacctcgtg ttttgtcttt ttttcattac caatgtgtgc atgtgaggaa
     2041 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttgcgcgact ggaaaccaga
     2101 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaatc tctggatatc
     2161 agtcagttgt ctacttcttg ccttctgtgt ctggatgcta tttagcgcag ttaccgttaa
     2221 tctcaataaa attggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2281 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2341 acgccgatgg acggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2401 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2461 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2521 caaacaaggg agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2581 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2641 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2701 gctactggcg attgccacga tcgccgcatg gtcagggatg aatgatatcg ccagttcacg
     2761 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     2821 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     2881 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttatcgg
     2941 tgccatcgcg cggtcggttg gtggtgctat ttccgataag ttcggcggcg tgcgggtgac
     3001 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3061 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgactgc
     3121 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3181 ttatcgggta aagatgaaag gcggtagtga tgagcaagct cagaaagaag ccgtcaccga
     3241 aacggcggcg gctctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3301 gcaggcgttt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3361 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3421 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3481 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3541 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3601 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3661 ccacggtgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3721 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3781 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     3841 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaagcaaca
     3901 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     3961 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4021 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4081 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4141 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4201 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4261 catcatcgcc tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4321 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4381 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4441 aatgggccat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4501 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4561 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4621 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4681 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4741 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt
     4801 ggccttcccc tactttggca gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     4861 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     4921 ggtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     4981 tgaaaacagt gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5041 aattaccggc gtgccgcgcc agtatattga aaccatcgct cgtgaatttg ccgatactgc
     5101 ccataaaacg catgggcgct caatgattat cctcggcgct ggcgtcaacc actggtatca
     5161 catggatatg aactaccgtg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5221 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5281 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccaaatga acagcacctc
     5341 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5401 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5461 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5521 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5581 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5641 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5701 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacggaat
     5761 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     5821 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgttc tgcccaccgc
     5881 cacctggtac gaaaaagacg atatgaacac ctcggatatg catccgttta ttcatccgct
     5941 ttctgcggcg gtcgatccgg cgtgggaatc acgcagcgac tgggaaatct acaaaggtat
     6001 cgccaaagca ttttcgcaag tgtgcgtggg tcatcttggc aaagaaaccg acgtggtatt
     6061 acaaccccta ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6121 gcgcaaaggc gaatgcgatc tgattccagg caaaaccgcg ccgaatattg tggcggtgga
     6181 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6241 tggcaatggc ggtaaaggga tttcgtggaa tacgcaggat gaaatcgatt tcctcggcaa
     6301 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6361 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6421 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6481 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6541 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6601 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6661 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6721 cgtcagtgag atgcgccaga taccgccaaa cggcttcccg gaaaaagcac ttaacttcct
     6781 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     6841 gctctctcgc ggtggaccga ttgtctggat cagcgaaacc gatgcccgag aactgaccat
     6901 tgtcgataac gactgggtgg aagtatttaa cgccaacggc gcgctgactg cccgcgcggt
     6961 ggtcagccaa cgtgtaccgc cgggcatgac catgatgtat cacgcccagg aacgcattat
     7021 gaataatcct ggttcggaag taactggcat gcgcggcggc attcataact cggttacccg
     7081 cgtttgcccg aaaccaacgc atatgattgg cggttacgcg cagctggcct ggggctttaa
     7141 ctactacggc accgtcggat cgaaccgcga tgagttcatc atgatccgca agatgaagaa
     7201 cgttaactgg ctggatgatg aaggtcgcga tcaggtacag gaggcgaaaa aatgaaaatc
     7261 cgttcacaag tcggcatggt gcttaacctc gacaaatgta tcggctgcca tacctgttcg
     7321 gtgacctgta aaaacgtctg gaccgggcgc gaaggcatgg agtacgcatg gtttaacaac
     7381 gtcgaaacca aaccgggcat tggttatccg aaaaactggg aagatcagga agagtggcaa
     7441 ggcggctggg tccgcgatgt gaatggcaag atccgtccgc gtctgggcag caagatgggc
     7501 gtaataacca aaatcttcgc caacccggtg gtgccgcaga ttgatgatta ctacgaacct
     7561 ttcactttcg actacgaaca tttgcatagc gcaccggaag gcaaacatat ccctactgct
     7621 cgcccgcgct cgctgattga cggtaagcgg atggacaaag tgatctgggg gccaaactgg
     7681 gaagaactgc tgggcggcga gtttgaaaaa cgtgcccgcg accgcaactt cgaggccata
     7741 caaaaggaga tgtacgggca gtttgaaaac accttcatga tgtacctgcc gcgcctgtgc
     7801 gaacactgcc tcaatcccag ttgcgtggcg acctgcccaa gcggcgctat ctacaaacgc
     7861 gaagaagacg gcattgtgct gattgatcag gataaatgcc gtggctggcg tttgtgcatt
     7921 agcggttgtc cgtacaaaaa aatctacttc aactggaaaa gcggcaagtc agaaaaatgt
     7981 atcttctgtt atccgcgaat tgagtcaggc caacccaccg tgtgctcaga aacctgcgtg
     8041 ggtcgcatcc ggtatctggg cgtgctgctt tacgacgccg accgcatcga ggaagcggcg
     8101 agcaccgagc gcgaagttga cctctatgaa cgtcagtgcg aagtgttcct cgatccacac
     8161 gatccctcag tgatcgagga agccctgaaa caaggtattc cacaaaacgt gattgaagct
     8221 gccctgcgtt cgcctgtcta caaaatggcg atggactgga aactggcgct accgctgcac
     8281 cctgaatatc gcaccctgcc gatggtctgg tacgttcctc cgctgtcgcc gattcagtcc
     8341 tacgcagatg cgggcggttt gccgaaaagc gaaggcgtgc tgcccgccat cgaaagcctg
     8401 cgtattccgg tgcaatatct cgccaatatg ttgagtgccg gcgataccgg tccggtactg
     8461 cgggcgctga aacggatgat ggcgatgcgc cactatatgc gttcacaaac cgtggaaggc
     8521 gttactgata ctcgtgccat cgacgaagta ggcctgagcg tcgcccaggt cgaagagatg
     8581 tatcgttacc tcgccattgc caactatgaa gatcgttttg tcatcccgac gagccatcgg
     8641 gaaatggcgg gcgatgcctt cgcagaacgc aacggctgcg gttttacctt tggcgacggt
     8701 tgtcacggct cggacagcaa attcaacctg ttcaacagta gccgtatcga tgccatcaac
     8761 atcaccgaag tgcgcgacaa agcggagggc gaataatgca gatcctcaaa gtgatcggcc
     8821 tgttgatgga gtatccggac gagctgttgt gggaatgcaa ggaggacgcg ctggcgttga
     8881 tccgccgcga cgcgccgatg cttacggatt tcacccacaa cctgcttaac gcgccgctgc
     8941 tggataaaca ggccgaatgg tgcgaagtgt ttgaccgtgg acgtaccaca tcgctgctgc
     9001 tgttcgaaca tgttcatgcc gagtcccgcg atcgcggcca ggcaatggtg gacctgctgg
     9061 cggagtatga aaaggtcggc ctgcaactgg attgtcggga actgcccgat tatctaccgc
     9121 tgtatctgga gtatttaagc gtactgcctg acgatcaggc gaaagaagga ttgctcaatg
     9181 ttgcgccgat cctcgccctg cttggcggtc gcttaaaaca acgcgaggca ccgtggtacg
     9241 cgttgtttga tgctctgctg caactggcgg gaagctccct ttcaagtgac agtgtcacaa
     9301 aacaagtaaa cagcgaagag cgcgatgaca cccgccaggc gcttgatgca gtgtgggaag
     9361 aggaacaggt gaagtttatt gaagataacg ccacggcgtg tgacagctcg ccgcttaatc
     9421 aatatcagcg acgctttagc caggatgtcg cgccgcaata tgtcgacatc agtgcgggag
     9481 gtgggaaatg attcagtatc tgaacgtctt tttttacgat atctacccgt acatttgtgc
     9541 gacggtgttt ttcctcggca gctggctgcg ctacgactac gggcagtaca cctggcgcgc
     9601 ctcctcaagt caaatgctcg ataaacgcgg gatggtgata tggtcgaatc tgttccatat
     9661 cggcattttg gggattttct tcgggcactt gttcggcatg ttaacgccgc actggatgta
     9721 cgcgtggttt ttgccagtgg cagcgaaaca actcatggcg atggtgctcg gcggtatttg
     9781 cggtgtgttg acgttgattg gcggcgcagg actgctgtgg cgcaggctga caaatcagcg
     9841 cgtgcgtgcc acttccacta cgccggatat catcatcatg agcattttgc tgatccagtg
     9901 tctgttgggg ctaagtacca taccgttttc agcacagtat cctgatggta gcgaaatgat
     9961 gaagctggtg ggttgggcgc aaagcattgt cactttccgt ggtggatcgt cagagatgct
    10021 caacggcgta gcgttcgtct tccgcctgca tctggtgttg ggaatgacca tcttcctgct
    10081 cttcccgttc acccgactgg tgcatgtgtg gagcgcgccg tttgagtact ttactcgtcg
    10141 atatcaaatt gtgcgttcgc ggcgataatt tcaccctggc cggatagccc ttcgctctcc
    10201 ggccacttgc aggctatgat agttctccag tcacaggaga acgttaatga aaccgcaggt
    10261 gtaccacgtc gatgctttta cctcacaacc gtttcgcggc aattctgccg gtgttgtttt
    10321 ccccgccgat aatcttagcg aagcgcaaat gcagcttatc gcccgcgagt taggccattc
    10381 ggaaactgct tttctgctgc acagcgacga cagcgatgtg cgcatccgct actttacgcc
    10441 aacggttgaa gtgccgattt gcggtcacgc gacagtagct gcgcactatg tacgtgcgaa
    10501 ggtgttaggt ttaggaaatt gcacgatctg gcaaacatcg ctggcaggaa aacatcgcgt
    10561 gactatcgaa aagcacaacg atgattatcg tatttcgctg gaacaaggta cgccgggctt
    10621 tgaactgccg ctggagggtg aagtacgcgc ggcgattatc aacgcattgc atcttactga
    10681 agacgatatt ctgccgggtt tgccgatcca ggtcgcgacg accgggcatt ctaaagtgat
    10741 gatcccattg aagccggaag tggatatcga cgctctttcg cctgacctta atgcgctgac
    10801 cgctatcagt aaacagattg gctgcaatgg tttcttcccg ttccagatcc gtccaggcaa
    10861 aaacgaaacc gatggtcgca tgttctcgcc tgcgattggt attgtggaag atccggtgac
    10921 tggcaatgcc aatggcccga tgggtgcatg gttggtacat cacaacgtat tgccccacga
    10981 tggcaaggtg ctgcgtgtta aaggccatca ggggcgcgca ttggggcgtg acggcgtgat
    11041 tgaagtgacg gtgacaattc gtgataacca accggaaaaa gtcaccattt ctggcgcggc
    11101 ggtgattttg tttcatgccg aatgggcaat taaactctga aatcgttgcc tgatgcgctt
    11161 cgcttatcag gtctacaacc gagctaatgt atgccggata aggcgtttac gccgcatccg
    11221 gcaacctgac ataaattatt ttcccgcctc cgggtgcgta tcaaacgccg ccatcaccag
    11281 cgctaactca tccacggtaa agccatgttt cgcatcatcc acgcccagac caaactgttc
    11341 ttgcatcaca gcatataatg acgccacatc cggtagattt cgctgctcca ccgcgtgccc
    11401 attttcataa tgggtaaaat gaaaattggt cagtgtcagc ttgccgccgt ccggcaaatg
    11461 gcggcacatc agcaaatgat ggcgaaaatg cgactgcggc cagtgcgccg accagaaatt
    11521 gcccatcaca taatcgcttt gttgctgctc gcagagatca aaacggtaca tcgactgcca
    11581 gtgctgatga tgattaaact gcaacaccca atcatcaccc tcctgcaaca accgatactc
    11641 tccgtgtggc gtggtctgca cgagatcggg aactaaacga atcggcgcgg ttagcgtctg
    11701 cccaccgaaa ccgacatcag caatccattt ttcctcttcc agttccacca acagcaaacg
    11761 atgggtgcgc ggcggtaatg ctggcggatt tgataacact acgcgcccta acaagctgcg
    11821 aacgttaaac cccagctcgc gtaacacccg ctcaaacacg ccattctgct caaaacagta
    11881 accgccacga cgggctatca ccagtttctc ttccagcgat tgattatcaa gctgtatttc
    11941 cctcggcagc aaaacgtcga ggttttcaaa cggaatggtg caattgtgtt tcaggtgcaa
    12001 tgcacgaagc gtatcaatat tgaccgcagc agctcccgac cagttaatac gggcaaaata
    12061 gtgattcaga atgggcgtca tcaggcgttt cccttagtgg ttttctcaac ttataacctg
    12121 gaatagacgc cgcaccggct attttgtgcg atcgcggaca tgcatcacgt tcgcaaatcg
    12181 ccaatcccgt taatttcgtt acttattgcg ttacggacta cgcttaaaaa aaccttcttc
    12241 gcgaggcgat accatgagcc gattcatccc catagaatta caccatgcca gccgtctgtt
    12301 aaatcatggc ccaacggtct tgatcaccag tttcgatgag caatcccagc ggcgtaacat
    12361 tatggctgca gcctggtcaa tgccggtgga gtttgaacct ccacgcgtgg cgattgtggt
    12421 agataaatcg acatggacca gggagttgat tgaacataac ggtaaatttg gcatcgttat
    12481 cccgggcgtt gcagcaacta actggacgtg ggcggtggga agtgtgtcgg ggcgtgatga
    12541 agataaattt aattgctatg gcattccggt tgtgagaggc ccggtatttg gtttgcctct
    12601 ggtcgaggaa aaatgtctgg cgtggatgga gtgtcgattg ctacctgcga cttctgcgca
    12661 agaagaatac gacacgctgt ttggcgaagt agtatcagca gcggcagacg cacgggtatt
    12721 tgtcgaaggc cgctggcagt ttgatgatga taagctcaat acgttgcatc atttaggtgc
    12781 tgggacgttt gttaccagcg gcaagcgtgt tacggcgggt taagcattca tgctgtaacc
    12841 aggtttcttt atcaaagcct ccatttgggg cgcgatttcg gcatcccaga tagcttgcca
    12901 agattctggt tgaatctgct gtagagcaat gcttatcgaa ctgtctttac tgttcagatg
    12961 acgaataata acgtcggtaa tatctgcagc aagtgctgct ttttgttgtt cgtccagttc
    13021 acgcggaaaa catttaatgt cgatgtgcgg catgattacg ctccttttga aagatgtcag
    13081 taaaattaac acacattgtg aatgtcatgt gtccggtaaa gcgtttatac gacaataatg
    13141 cccagggcaa gattacgaaa gcccgctccc cgtaaggact gacgccaggt agtttctgtc
    13201 catggctgct tttcgcatct tacgtcttaa ccctgccttg aataccttat cattcgtcaa
    13261 aatattaata gcaatgtgcc gtatccctga aaataattct gctgcatttc ctcttcttat
    13321 tttgcagtcg tcttcattca ttaccacgtc cagacgccag tgcagcttat tctccacatg
    13381 ccagtggttt cggattgctg tggcgaactt ctctgcggtt aaatcagcag aactgatata
    13441 atatctgacc gtcatttcga gctctttctt ttgttctgct attatggacc gaaaggagac
    13501 tgccacgcat aatttcttca gccccttcca ttcaaacgtg aaatcaataa gttcatcagg
    13561 gacatcgcaa acaatatgaa gacggatttc ttctctgccg tgactctttt cactcattgc
    13621 gtaactgtca tgcgctggat tatttaattc tttcagcgga aatttttcct caaaggcttt
    13681 atttagccgc ccctggtttc cttttaccgc gaataaataa tcacctccct gtttttgtat
    13741 cttctctgca atatctttct ggcaacccat cgcatcagtt gtgatgattt ttcctttaat
    13801 atccagcatg ttaagaagtt caggaatggc tgtaatctca ttagatttct catccgtctt
    13861 aatctgcccg agaatcagac tgtgcattgt tgagaacgca ctaatgacat gaatcgctcc
    13921 cctgcggcga ctcttgtcat aagagtgccg gagcgttttt ccatcaattg caatgacgtc
    13981 tttatcatct gaagaatggc agtcacgcat ccagttaata aagcactcgt gaaattttgc
    14041 aggactgata caggatacaa ctctggcaat agtatcgtga acaggaatac cattttcaaa
    14101 atcaccatat
//
LOCUS       DAOFQD010000120        13397 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.120, whole genome shotgun sequence.
ACCESSION   DAOFQD010000120 DAOFQD010000000
VERSION     DAOFQD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.120"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            6..1940
                     /gene="yegI"
                     /locus_tag="QBF46_003730"
     CDS             6..1940
                     /gene="yegI"
                     /locus_tag="QBF46_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDQ6675859.1"
                     /translation="MKVFTSTDELTTLGRELGKGGEGAVYDIEEFVDSVAKIYHTPPP
                     ALKQDKLAFMAATADAQLLNYVAWPQTTLHGGRGGKVIGFMMPKVSGKEPIHMIYSPA
                     HRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDSDS
                     FQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTENHDNFGLALLIFHVLFGGRHP
                     YSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQAF
                     TESGVATGRPTAKAWVAALDSLRQQLKKCIVSAMHVYPAHLTDCPWCALDNQGVIYFI
                     DLGEEVITTGGNFVLAKVWAMVMASVAPPALQLPLPDHFQPAGRPLPLGLLRREYIIL
                     LEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRAKM
                     DYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQKF
                     LEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKAS
                     CERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMPLM
                     EPLRQAAEKLAQAQADLSRC"
     gene            complement(1953..3305)
                     /gene="yegD"
                     /locus_tag="QBF46_003731"
     CDS             complement(1953..3305)
                     /gene="yegD"
                     /locus_tag="QBF46_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDQ6675860.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLCRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLTRILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            3439..4287
                     /gene="alkA"
                     /locus_tag="QBF46_003732"
     CDS             3439..4287
                     /gene="alkA"
                     /locus_tag="QBF46_003732"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDQ6675861.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDMARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRLYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(4396..7713)
                     /locus_tag="QBF46_003733"
     CDS             complement(4396..7713)
                     /locus_tag="QBF46_003733"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDQ6675862.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAISMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNIESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWNCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            8031..8672
                     /gene="udk"
                     /locus_tag="QBF46_003734"
     CDS             8031..8672
                     /gene="udk"
                     /locus_tag="QBF46_003734"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDQ6675863.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERIKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            8764..9345
                     /gene="dcd"
                     /locus_tag="QBF46_003735"
     CDS             8764..9345
                     /gene="dcd"
                     /locus_tag="QBF46_003735"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDQ6675864.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            9367..11220
                     /gene="asmA"
                     /locus_tag="QBF46_003736"
     CDS             9367..11220
                     /gene="asmA"
                     /locus_tag="QBF46_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDQ6675865.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRNLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEIIQLQGKLNQGVVSLPGTLD
                     ATASHPRIVFHPRLENVEIGSILNAFNYPISLTGKMSLAGDFSGADIDAEAFRRNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDHFSTDLTLDNGVVT
                     LDDMQGESPMLALSGAGTLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11494..13077)
                     /locus_tag="QBF46_003737"
     CDS             complement(11494..13077)
                     /locus_tag="QBF46_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDQ6675866.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
BASE COUNT         3245 a         3479 c         3523 g         3150 t
ORIGIN      
        1 caaatataaa agtatttaca tcgacagatg aattgaccac tcttggccgt gaactgggca
       61 aaggtggtga aggcgcggtt tatgatatcg aggagtttgt cgatagcgtc gccaagattt
      121 atcacacgcc gccacccgcc ttaaaacagg acaaacttgc ctttatggct gcgacagctg
      181 acgcgcagtt gttgaattat gtcgcctggc cgcagacaac gcttcacggt ggacgaggcg
      241 gaaaagttat cggttttatg atgccaaaag tttctggtaa agaaccgatt catatgatct
      301 atagcccggc acatcgtcgc cagagttacc ctcattgtgc gtgggatttt ctactctatg
      361 ttgcgcgcaa tattgcttca tcttttgcta cggttcacga gcacgggcac gtcgtgggtg
      421 acgtaaacca gaacagtttt atggtaggtc gcgacagcaa agtggtactg attgatagcg
      481 actcctttca aattaacgcc aatggcacac tgcatttatg cgaagtcggc gtgtcgcatt
      541 ttacgccgcc agagctacaa accttgccat catttgtcgg ttttgaacgc acagaaaatc
      601 acgataattt tggccttgcg ttgctaattt ttcacgtctt gtttggtggg cggcatcctt
      661 attccggtgt gccgcttatc tctgatgcgg gtaatgcgct ggagacggat attacccatt
      721 tccgttatgc ctacgcgtca gataatcagc gacgtggttt aaaaccgccg ccacgatcta
      781 ttccgctatc gatgttaccg agtgatgttg aagccatgtt tcagcaggca ttcacggaaa
      841 gtggcgtggc aaccgggcgt ccgacggcta aagcgtgggt agcggcactg gactctctac
      901 gccaacagtt aaagaaatgt atcgtttcag caatgcatgt ttaccccgct catttgactg
      961 actgcccgtg gtgtgcgctg gataatcaag gtgttatcta ttttattgat ctcggcgaag
     1021 aggtcattac caccggcggg aattttgtgc tggcgaaagt ctgggcgatg gtgatggcgt
     1081 cagtagcacc gccagcattg caactgccat tacccgatca tttccaaccg gctggcaggc
     1141 cgcttccttt aggcctgtta cggcgcgaat acatcattct gcttgagatc gcactgtcag
     1201 cattatcgct gctgctttgc ggccttcagg cagaaccgcg ttatattatt ttggttcctg
     1261 tgctggcggc tatctggatt attggcagtc tgacaagcaa agcgtacaaa gcagaagttc
     1321 agcaacgccg tgaggcattt aatcgtgcga aaatggacta tgaccattta gtccgccaga
     1381 tccagcaggt aggcggcctg gaaggtttta tcgccaaacg gacgatgctc gaaaaaatga
     1441 aggacgaaat tctcgggtta ccggaagaag agaaacgcgc tctggcagca cttcatgaca
     1501 ctgcaaggga acggcagaag cagaagtttc tggagggatt ttttattgat gttgcctcta
     1561 ttcccggcgt tggccctgcg cgtaaagcgg cgttacggtc ttttggtatt gaaacagccg
     1621 cagatgttac ccgtcgtggc gttaagcaag tgaaagggtt tggcgaccat ctgacccagg
     1681 cggttatcga ctggaaagcg agctgtgaac gccgttttgt gttcaggccg aatgaagcga
     1741 taacgccagc agacagacaa gcggtaatgg cgaaaatgac cgccaaacga catcggctgg
     1801 aatcggcgtt gacagtcggc gcgacagagt tgcagcgatt ccgccttcat gctccagcac
     1861 ggaccatgcc gttgatggaa ccgttacgtc aggcagcaga aaaactggct caggcgcagg
     1921 cagatttaag ccgctgctga gccatttttc aattaacgaa acaccacttc cgcccagcgt
     1981 gccagcccgg cggtgacgga gccaaagtca tcgccgcctg caatcggaat gcccggcaac
     2041 tgttctgcca gcgctttttt aatcagcgga gaacgggcgc taccgccggt caggtagata
     2101 acgtccggtt tttcctgggc gttatccagg gccagttgca cctgttccag aatccgcgtc
     2161 agtggctggc tgagggcgct ttccagccct cgctggctaa tcagcgtagc cagttcattg
     2221 ctgataaacg gcagtgaggc gcgggtttcc gctacgcttg aaagagcaat tttgcactct
     2281 tctgcgctac gcaccaggcg atagcttaaa cgctgacgcc agactttctg taacagggcc
     2341 actttttccg gttcgcgggc atcgcgtacc agatcgttaa gcagacgacc gttggcacta
     2401 ctgtagaaat cactctgcgc aggtacgtcg ttgatggcaa ccgcattcca ccacggcagg
     2461 atcggcaggg cgatgccttt ttcggtttcg ccacccatac ccagcagtgg catcaggttt
     2521 ttaaacgcca gggcgatatc cagatcgtta ccgccaatac gacaaccact gtgacccagc
     2581 aggctggctt cacgatcgag acgcgaacgc cactgcggtc ccatcagcag caatgaacag
     2641 tcagtcgtac caccgccgat atccaccacc agcacccgtt tttcttcctg caaggtggct
     2701 tcgtaatcca gcccagccgc gaccggctcg tactggaata ccacgtccct gaatccggca
     2761 cgcttcgccg cgcgttccag aatcccttgc gcctgggcgt ttgcttcatc gccgcccagc
     2821 ccctggaagt tgatcgggcg accaatcacc gcctgagtaa tcgcttctgg cagctgcgcc
     2881 tgcgcctgct ggcgaatgtg caacatcatt gcgcagacca gatcctcaaa cagcgctacc
     2941 tgctgcggtt ttaagccgct ggcaccgagg aacgattttg gtgatttcac aaaccacact
     3001 tcttctggat catcaatgta ctgtgccagt gaggaaagac cgaactgcac gcttttcgcc
     3061 gtaacatcga tatcttcttc gcggttataa cgaatcgccc gacataacag cgcctgcgtt
     3121 tcatcgtcgt ctgccggaac atcatgatgg cggtacagcc attcacttac tgcttcacgc
     3181 gttggcgcgc aaagcattga aggcagcagc gtgctgtcgt tttccatttt cagtaattgc
     3241 ggtttaccgt cacgcatgac cgccactgaa cagtttgctg taccgtaatc aaaaccaata
     3301 aacacgaatt aatccccatg ccggtgaaga aggggcgtga ctttagcgaa atgttgccgt
     3361 cgcgacaacc ggaatatgaa agcaaagcgt agcgtctgaa taacgtttat gctgaaagcg
     3421 gatgaataag gagatgcgat gtataccctg aactggcagc cgccgtatga ctggtcgtgg
     3481 atgttgggat ttctcgccgc ccgtgcagtg agtggtgtgg aaacggtcgc tgacgattat
     3541 tatgcccgta gtctggcggt gggcgaatat cgcggcgtgg tgactgctat tccggatatg
     3601 gcccgtcata ctctgcacat aaatttaagt gcaggtttag aacctgttgc cgctgagtgt
     3661 ctggcgaaaa tgagccgcct gcttgatctg caatgtaacc cgcagattgt taacggtgcg
     3721 ttgggcaagt taggcgcggc gcggcccgga ttgcgtttac ccggctgtgt tgatgctttt
     3781 gagcagggtg tgcgggcgat tttaggccaa ctggtgagcg tggcgatggc ggcaaaattg
     3841 actgccaaag tggtacagct ttatggcgaa cggctggatg attttccgga gtatatctgc
     3901 ttcccgacgc ctcagcggct ggcagcagcc gacccgcagg cattaaaagc gttaggtatg
     3961 ccgttgaaac gggcagaggc gctgattcat ctggcaaatg cggcgctgga gggcacctta
     4021 ccaatgacaa taccgggcga tgtggagcag gcgatgaaaa cgctgcaaac ttttccgggt
     4081 atcgggcgct ggacggcgaa ttatttcgcc ttgcgcggct ggcaggcgaa agatgttttt
     4141 ctgccggatg attatctgat taaacagcga tttccgggga tgacaccggc acaaatccgc
     4201 ctttatgccg aacgctggaa gccctggcgt tcttatgcgc tgttgcatat ctggtatacg
     4261 gaaggctggc aaccagacga agcatgacgt tggtcgccgg atgcggtgcg agcgccttat
     4321 ccggcctgga gaatgagcga tttgtaggca tgataagacg cgttagcgtc gcatcaggcg
     4381 atggggaagc acgcctcagt taatcgcgaa ataactacta ttcaccagca aatccagcgg
     4441 ttgggcatcg gcaatcacat caccataaat cagatcgacg ccgatgccag aaagcgtatc
     4501 catcactaag ggtaaaacga ctggcccggc gatggttttc atcccgagtc gctgagcgtg
     4561 cccctgaata atcgtaatca gcatctcatc catcaaatta ccctgcacgt tggcgcataa
     4621 ctcaccatca agtagcaggt aatctgccat attcgctttc agcgagttga agatttgcag
     4681 atcgcggccc acctggctga gcactatccg acatcccgcc agccgcagtt tttgcacgct
     4741 ttctgcgtga tctaaaatcg cttcagccgg aataatcaga tgtaataacc gtggtggtag
     4801 agggctattt tccagctgct caagcagatc attcaccagc gtggcgctac tcaaaccggc
     4861 aacggaaaga gggagggcga tgcttatgcc tttactggca accgctttgg cggcctgctg
     4921 gaaaaaatcg tggaataccc gtcggtcaag agcatggcta agtgccggat cgctgaagct
     4981 acgacgaaat gtttgttcat caataatctc gccttcgcaa ttccagagct taagtgaaat
     5041 cagccacaaa ttacgtgctt ccgggatccg tggcgaagcg acaccgtggg cgatcatcat
     5101 caactgattc tctttaatca tccgccactg ttcatcaagc gacatcgctg cccgctcgct
     5161 atgtgcggca gcttgctgcg gttcgtaaat cgtcacccgg ccccggccac catttttgga
     5221 ggcataacag gcgatatcag cctgcgacat cacttcagcc gcctgatgat tgttgtcatc
     5281 aatcaaggta atcccggcac tggcacctac ccgatgtaca cgtccttccc atataaagtg
     5341 ataatcattc acggcactga taatgcgtgt agcgataaaa cgcgcacttt cgatattaca
     5401 gtctggcaac agtagaccaa attcatcacc accgagtcgc gccagcacgt cgctggagcg
     5461 cagcatactc agcatcaacg acgccagttc gcgcagtaaa gcgtcacccg ccgcatgccc
     5521 ggcgctgtca ttcaccgctt taaagcgatc aagatcgata aacaccaggg catgtcgctg
     5581 atgtgtactg tttaccgttt gcagcaggat acgcagttgt ttctcaaaac tggcgcggtt
     5641 ggcgagatgc gtcagtgcat catgggaggc gctgtagctc agctggcgca gcattttgcg
     5701 tgattcggtg acgtcctgaa tcaccagaac cgaaccaata ttgctgccgt ccagagtact
     5761 taacggcgta atactgtaat gcacgtcgta gctgccgccg ctccgacagt gcaacaccac
     5821 atcttgttca atcgcggaac gtgaggtgtc ggcactgtaa atgttctcca ttaatggtcc
     5881 gttgtcgcca aaagtaatat gcaacaccgt caggagcgga acacctaacg cttcttcctg
     5941 cgtccagccg ctcatcttct ccgccactgg attcataaag gtaattttca tcgccatatc
     6001 aatacagacc acggcttcac cgatggaatc aagcgtaatg tgcaggcgct ctttttcctg
     6061 aaacaatgcc tcgttaagct gtttaacctc ggtcatatcc atattaatgc cgagcaggcg
     6121 ttcgacttcg ccttctttat tcagtacccg gttggcgagg gcgcggatat ggcgaatacc
     6181 gtcttttacg gtaatgcgaa attccagctt aaagggcgag cgtgattgca acgaatcacg
     6241 aatcactttt tcggcatgct ggcgatcttc cggcagcacg cactcgtacc acacctgcca
     6301 gttcggtttg atatgcggag gaatttcata cagctcgaac atccgcttat cccagctaaa
     6361 aatattcggc ttcagctccc actcccagat gccaatcccg cccgcttcgt tagccagcgt
     6421 gatgcgctcc atcagttgct gattcacctg ttcggtgcgt tttagctcgt taatgtcttc
     6481 aatctgagcg ataaaataga gcggcgtgcc atccgtgtgg cgcaccagtg agacggcaag
     6541 caacgcccag acaacatcgc cattgcggtt gtagtagcgt ttttccattg aataggtgtt
     6601 tatttcaccg cttatcagct tttcaacctg ttggagatct ttattgagat cctccggcca
     6661 ggtcagttgc tgaaaggtga gtccgcgcag ctcttcctga ctgtacccga gaaactggca
     6721 gagcgctttg ttggtttgca gccattgtcc ctcggtgccc actaacgcca tactgatagc
     6781 ggaatattcc atggcgttcc gaaaacgggt ttcgctttcg gaaatgtgtt tgcgttccgc
     6841 acgaaaggca tacatcacca tcgtcatgat gttggcgggc agcaggatca gcaaaaacgg
     6901 tagccacggc atatggctca tcaggtatgt acgcggcgta gcaagcaggg agggatccgc
     6961 ggccatcatc agcgacacca tcatcaccgt ggtaaggaag atcaaaaagg cttccattcg
     7021 tggcaggcgc acggcgctcc acatcaacag cacaataatg aaagtaaaag gccacggcag
     7081 gtacaacata gaaagccagc ttaacgtcag cgtgatggct aacgtgagca gtgactcaaa
     7141 aagtaaccgt gggttgcgat ggcgcagcag atagtgtggc ttaaataaca atcccagcgg
     7201 caccagtgcc agagcgccga tggattctga cagtacccat atcaaaaatg ccctgagagg
     7261 atcgtctccg ggcgtcagca ggacaaccag aacacccccc aacagaggcg gaacaatggc
     7321 gctgccgagt gccagacgca gccagtcagc cagattttgc aaggggttat accacggcag
     7381 caatttacgt agcagcactg ccccgaccac ggcttcaaca atattgatgg tcgtccaggt
     7441 catgttcagc gagctggtgg aaaaaagcag gatggatgcg gcgatatttc ccagcgaaca
     7501 gctcagcgca attcccggcc acatgcgccc ggcatggcga taaaacgcca ccatcatgat
     7561 ggacgtcggg aaccacagtg gggcgagttg ggtgccaaac tgcgaaagct caagcgagaa
     7621 aagggtaaag ataaacgaga ctaaacctaa actgaccagg tgaagcagcg ggtggggcag
     7681 ggcaattaat acatgctgtg attgtttgct cattaccgct ttatccgcaa gaccaggtcg
     7741 ccagtattcg tttctgtgtt tatgctagta caaaagtttt acattttata tgcgaattgc
     7801 tcataaagtg acgtaaaggc ggataatttg cgcaactgcg tataacattt tttaccttgt
     7861 ataaaactga tcaacgtaat ttgccaccgg gaattcttcg tcaacaatta actctgagcg
     7921 cgcggcaacg ctattcgact ggtatcaggc ggatgaaatc cctataattg ccgcgtttgg
     7981 cgcttcgtcg cccccttcct aacatccagg ttaatcaggt cgctaaatat atgactgatc
     8041 agtctcatca gtgcgtcatt atcggtatcg ctggcgcatc ggcttccggc aagagtctta
     8101 ttgccagtac cctttatcgt gaattacgtg agcaagtcgg tgatgaacac atcggcgtaa
     8161 ttcccgaaga ctgctattac aaagatcaaa gccatctgtc gatggaagaa cgcattaaga
     8221 ccaactacga ccatcctagc gcgatggatc acagcctgtt gcttgagcat ttacaagcgt
     8281 tgaaacgcgg ctcggcaatt gacctgccgg tttacagcta tgttgaacat acgcgtatga
     8341 aagaaacggt gacggttgag ccgaagaaag tcatcattct cgaaggcatt ttgttgctga
     8401 cggatgcgcg tttgcgtgac gaacttaact tctccatttt cgttgatacc ccgctggata
     8461 tctgcctgat gcgccgcatc aagcgtgacg ttaacgagcg tgggcgttca atggattccg
     8521 tgatggcgca atatcaaaaa accgtgcgcc cgatgttcct gcaattcatt gagccttcta
     8581 aacaatatgc ggacattatc gtgccgcgcg gcgggaaaaa ccgcattgcg atcgatatat
     8641 tgaaagcgaa aataagtcag ttctttgaat aagcttgata aattgtgtac cgttcagtga
     8701 taacctggta tgcccttgac gtaattggcg ttaagggagt gatgcgcgaa aggagaaaat
     8761 gccatgcgtc tgtgtgaccg agatattgaa gcctggcttg atgaaggccg tttgtcgatc
     8821 aacccacgtc cgccagtgga gcgtattaac ggcgcgacgg tggatgtacg cctgggcaat
     8881 aaatttcgta ccttccgtgg tcacacggca gcgtttatcg atctgagcgg tcccaaagat
     8941 gaagtgagcg ccgcgcttga ccgcgtgatg agcgatgaga tcgttctcga cgagggcgag
     9001 gcgttctatc ttcacccagg agagctggcg ctggcggtga cgctggagtc ggtgacgctg
     9061 ccagccgatc tggtgggctg gctggacggg cgttcctctt tggcgcgtct ggggcttatg
     9121 gtgcacgtca ccgcgcaccg catcgatccg ggctggtctg gctgcattgt gctggagttc
     9181 tacaactccg gtaagctgcc gctggcgctg cgtccgggca tgttaattgg tgcgctgagc
     9241 tttgagccgc tttccggccc ggcggcgcga ccttacaacc gccgtgaaga tgcgaaatat
     9301 cgcaaccagc agggcgcggt agccagccga atcgataaag actaattcag gcccattgag
     9361 gataccatga gacgatttct gacgacgctg atgatactcc tggtcgtgct ggtggccggg
     9421 ttatctgcgt tagtgttgct ggtgaatccg aatgatttcc gcgactatat ggtcaagcaa
     9481 gttgctgcac gtagcggtta tcaattgcag ctcgacgggc cactgcgttg gcacgtctgg
     9541 ccgcagctta gtatcctctc cgggcgaatg tctctcaccg cccagggcgc aagccagcca
     9601 ctggttcgcg ccgacaacat gcgtctggac gtggcgcttt taccactact gagtcatcaa
     9661 ctgagcgtta agcaggtgat gctaaaaggg gcagtgatcc aactgacgcc gcagacggaa
     9721 gcggtgcgca gtgaagacgc tccggttgca ccgcgcgaca ataccttgcc ggatctgtca
     9781 gacgatcgcg gatggtcgtt tgatatatcc agtcttaagg tggcggacag cgtgctggtg
     9841 ttccagcatg aagatgacga gcaggtgaca atccgcaata tccgcctgca aatggaacaa
     9901 gatccccaac atcgtggctc atttgagttc tccgggcggg ttaatcgcga tcagcgcaat
     9961 ctcacaatat cccttaacgg tacggtggat gcttctgatt atccgcatga tttaacggcg
    10021 gctattgaac aaattaactg gcagttgcag ggtgccgatt taccaaaaca aggtattcag
    10081 gggcagggga gtttccaggc ccagtggcag gagtcacata aacgcctttc atttaaccaa
    10141 atcagcttga ccgccaatga cagcacgctg agcgggcaag cacaggtcac gctgacagag
    10201 aaacctgaat ggcagctgag actgcaattc ccgcaactga atcttgacaa cctcatcccg
    10261 cttaatgaaa ccgcgaatgg tgaaaacggt gccgcgcagc aggggcagag ccaatcaacg
    10321 ttgccgcgcc cggttatttc ttcgcgtatt gatgaaccgg cctatcaggg actgcaaggc
    10381 tttacggctg atattttgtt gcaggccagt aacgtgcgct ggcgcggaat gaattttaca
    10441 gatgttgcca cgcaaatgac caacaagtcg ggtttgctgg agattatcca gttgcagggc
    10501 aaacttaacc agggtgttgt gtcgttacct ggtacgctgg atgccacagc ctctcacccg
    10561 cgtatagttt tccatccacg gctggaaaat gtcgagattg gttccattct gaatgccttt
    10621 aactacccca tttcgctgac cggtaaaatg tcactggcgg gggatttctc tggtgctgac
    10681 atagatgccg aagcatttcg ccgtaactgg caagggcagg cgcatgttga gatgaccgac
    10741 acacgtatgg aagggatgaa cttccagcag atgattcagc aagcggtaga gcgtaatggc
    10801 ggtgatgtga aggccgctga aaactttgat aacgtgaccc ggcttgatca cttctctacc
    10861 gatttgacgc tcgacaatgg tgttgtgaca cttgatgaca tgcagggcga gtcgcccatg
    10921 ctggcgcttt ccggggctgg tacgttgaat ctggcagatc aaacctgcga cacccagttt
    10981 gatattcggg tcgtgggtgg ctggaacggg gaaagtaaac tgattgattt cctgaaagaa
    11041 acgccagtac cgctgcgggt ttatggcaac tggcagcaac tcaattacag cctgcaagtg
    11101 gatcagttac tgcgcaaaca tctacaggac gaagcgaaac gtcgcctgaa tgactgggcc
    11161 gagcggaata aagattcccg caatggcaaa gatgtgaaga agttgctgga gaagatgtag
    11221 gttaccaggt gaccagtcct gtaggcctga taagacgcgt aaagcgtcgc atcaggcgtt
    11281 cgcggcggca ccattatatt ctgaatatgt aagatcgaag gctgttccgt tcacttgatg
    11341 ttcctcacta ctatggaacg ctcaagccgg tgaacgtgcc agggtggctc aatctccacc
    11401 accctggcaa cccgggctcc cggcggtaaa tcgccgctgg cgcggttccc tcatcttatt
    11461 cctttcggct atcgggtaaa atgaccgata ccatcacacc tcgtactcca tctcgccatc
    11521 cttacgcagc ggtataatct gcaccttctg cacgcgatgg ctttctacct gcaacgtttt
    11581 aagcaaataa tcccccacct gaacttcttc gccaggtttt ggaatacgct gcaaatactc
    11641 catcaacagc ccggcaatgg tgtgatattc acgtttttca tccagcggca gcggcacata
    11701 ttgcaccaga tcttccagcg gcatatgacc attcgccgtc caggaaccgt ccgcattctt
    11761 ctgaatatca tggcgggcgt cgatctcttc cacttcgttc ggtaagttac cggcaatggt
    11821 ttcagtgacg tcacttaatg tcacaatccc ttccaccgag ccaaactcat ccaccacaaa
    11881 agcaaagtgc gtgcgggcat tacggaactg ctctagggca ggtaacaacg gcaaggtttc
    11941 cgggaacacc agcggctggc gaatcaacac ccgcaggttg agcggttcgc cgcgcagaga
    12001 ctgttgtagc aggtcgataa cgtgaacaac accgagcaaa tcttctgcgt catcaccgtc
    12061 ggtaaccacc agccgcgtat gctgatttcg ctccagcaac tggcggattt cttcttccgg
    12121 cgcgttgaga tcgatatgct caatatcgtg gcgcgacgtc ataatgctgc tgacggtacg
    12181 ctggttaaga ttaagtaccc gctcaatcat ccgccgttcc tgcggattaa agatctgctg
    12241 gtttccatga tccaccagca tggacgcggt ttcggcgtcc agttccgcat cttctttttg
    12301 cccgctaagc aggcgcatta ccgcttcggt agtccgctgg cgtagcgtct gattagcgga
    12361 aagaaaacgt cgccggttaa agatagccaa ctgattgagt gcctcgatca tcaccgagaa
    12421 accaatggca gcgtacaggt agcctttcgg aatgacgaag ccgaaacctt ctgccaccag
    12481 gctaaagcca atcatcaaca ggaagcttaa gcagagaata acgatggtcg ggtggctatt
    12541 aacgaactgg gttaacggct tgctggccat caacatcagg ctgatagcga taaccacggc
    12601 ggccatcatg accagtaaat ggtcgaccat cccgacggcg gtaatcaccg agtcgagtga
    12661 gaagatggcg tccagtacca caatttgtgt caccaccccc cagaacttcg cacctttgcg
    12721 ttgtgtggga ttattgctgt ctttcccttc cagccgttcg ttcagctcca tcgtggcttt
    12781 gaacaacagg aagaaaccac caaacagcat gattaagtcg cgggcgctaa aggtaaacga
    12841 gcggaagctg aacagcggtt gcgtcagggt gaccagccag gagattgacg ccagcagtaa
    12901 caggcgcatt aacatcgcca gcagcagccc ggtaacccgt gcgcggtcgc gctgcttcgg
    12961 cggtagtttt tcggcgagga tggcaataaa gaccaggtta tcaatgccga ggaccagttc
    13021 gatcacaatc agcgtgatta acccggccca gatagacgga tcggcaatcc attccatagt
    13081 aagtacaaaa cctattcgtt gtatgacaaa tgtcacaatt cgatcatgga taaatcgggg
    13141 caaaaatgca atgttgcagc actatttatc tcatattatt tgagggtaat aacatcacat
    13201 tatccctgaa ttaaaagtgg taataataaa acattattta agaaatatcg catgtggaga
    13261 cgcaatatta aatattgtca acctaaagaa actcctaaaa aacatattga atgacactta
    13321 atataattct taaaaataac caattgccga attgttacct tgcctgctat tccgttagct
    13381 gtaacacttc ctcctgc
//
LOCUS       DAOFQD010000121        13394 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.121, whole genome shotgun sequence.
ACCESSION   DAOFQD010000121 DAOFQD010000000
VERSION     DAOFQD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.121"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            62..848
                     /locus_tag="QBF46_003738"
                     /pseudo
     CDS             62..848
                     /locus_tag="QBF46_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            complement(989..1762)
                     /gene="uxuR"
                     /locus_tag="QBF46_003739"
     CDS             complement(989..1762)
                     /gene="uxuR"
                     /locus_tag="QBF46_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDQ6675867.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1977..3437)
                     /locus_tag="QBF46_003740"
     CDS             complement(1977..3437)
                     /locus_tag="QBF46_003740"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDQ6675868.1"
                     /translation="MTTIVDSNLPVARPSWVHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3518..4702)
                     /gene="uxuA"
                     /locus_tag="QBF46_003741"
     CDS             complement(3518..4702)
                     /gene="uxuA"
                     /locus_tag="QBF46_003741"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDQ6675869.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5042..6385
                     /gene="gntP"
                     /locus_tag="QBF46_003742"
     CDS             5042..6385
                     /gene="gntP"
                     /locus_tag="QBF46_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDQ6675870.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6559..7461)
                     /gene="fimH"
                     /locus_tag="QBF46_003743"
     CDS             complement(6559..7461)
                     /gene="fimH"
                     /locus_tag="QBF46_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDQ6675871.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSLAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7481..7984)
                     /gene="fimG"
                     /locus_tag="QBF46_003744"
     CDS             complement(7481..7984)
                     /gene="fimG"
                     /locus_tag="QBF46_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDQ6675872.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGTTQGTIQAVI
                     SITYTYS"
     gene            complement(7997..8527)
                     /gene="fimF"
                     /locus_tag="QBF46_003745"
     CDS             complement(7997..8527)
                     /gene="fimF"
                     /locus_tag="QBF46_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDQ6675873.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8537..11173)
                     /gene="fimD"
                     /locus_tag="QBF46_003746"
     CDS             complement(8537..11173)
                     /gene="fimD"
                     /locus_tag="QBF46_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDQ6675874.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINSGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQYINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSADMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMNNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11240..11965)
                     /gene="fimC"
                     /locus_tag="QBF46_003747"
     CDS             complement(11240..11965)
                     /gene="fimC"
                     /locus_tag="QBF46_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDQ6675875.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQVAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12002..12541)
                     /locus_tag="QBF46_003748"
     CDS             complement(12002..12541)
                     /locus_tag="QBF46_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6675876.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12606..13160)
                     /gene="fimA"
                     /locus_tag="QBF46_003749"
     CDS             complement(12606..13160)
                     /gene="fimA"
                     /locus_tag="QBF46_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDQ6675877.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGAELTLDGATFSVQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         3268 a         3470 c         3206 g         3450 t
ORIGIN      
        1 actacagaaa aatttatcta tttttctaag catcagtgaa atactcataa aggtaactat
       61 tatgtctatg ccattaagca atgcccttca gagttaaatt attacagata atcacttcct
      121 tcatcatcca aaagtcgaca gtgagttaac acgtaaacat gaacgtgcac gcatggatac
      181 ggaaaatatc tatcttccgc ctcttgctcg tggcaataac cataattacg atggaaaaag
      241 cgttgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      301 agaaacatgc agagaattca atggtattac aacaacagga cgtatgctct atcgaaactt
      361 gaaaatcacc tcagctttag acgaaatata tggtggtata tgtaaaaagg cgcatgctgc
      421 cacagagtta gcaaaaggat tacggctaaa tttatttatg aaaggccctt tcgatcctgt
      481 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      541 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      601 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      661 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      721 aataattggt atagaagcct atcccgggtg atgaaaatgc accactcaaa cgtatgactc
      781 tgcaaccgtc atctcttcca gaactgtttg aattaaaaaa tggtgaatgg aattatattg
      841 gtaaataact accaggaaga atgagtacta acattcaggt tatacagata gctaaacaat
      901 cccctgcaat ttacaaagta atactcattg cgacttctta atttaagaaa gcgcaaggaa
      961 cgtttaccct tgcgcttatt ataataagtc aggcgtcgac tttatccagc ggccatgaat
     1021 caaacagata gccatcaaaa taaatatcgt caacgttcga gaattccagc agacgttgct
     1081 taacgttttc cagatgctgc cacattgcca gcttcgccgc tcgggcatct tttttgatta
     1141 acgcggcgag gatctgtttg tgatcgccca accactcttt gcgatacagg ctgtcatcca
     1201 gatggctgtg caactggatc cacattggat tgttttcccg ccactgccag gactgacgga
     1261 acagctccac cagcatgctg ttatgcgttg cttcggcaat agcgagatgg aactgcatgt
     1321 caccgctttc gctgctgccc ggcgcactgg aagccagttc acgctcttcc agttgcaatg
     1381 cctgacgcat tttgacgata tcttcgcggg tagcctgcag ggcggcaaac tcggcgatgt
     1441 tgctctcaag aagctgtcgc gcctgcaaca gctcaaaagg acccgcatca ttgcaaacgt
     1501 tggcatccgg actgtctgtg ttgtggctgc ctgagctatc aagaacatag ataccggcac
     1561 cccggcgcac ttccaccagc cctttgatct ccagcatgat cagcgcttca cgtaccaccg
     1621 tccgcgtgac atcaagcatt tctgcaattt cacgctccgg cggcaaccgt tcgccaggat
     1681 tgtacggcgt ctttatgatc agatcgcgga tcatcgcccc gacttcctgg taaggtcttt
     1741 gcgcagaggt ggcagatttc atggtgttgt gtccgttagg tgcggttaat caaatttttg
     1801 ccggatgcgg cgtgaacgcc ttatccggcc tacaacgggc aattttacgg cacccgttaa
     1861 tctcaaacta taccattagc actggaagtt ggcacgtaca ggtgggcaaa aaaacgaaaa
     1921 ctggtcaacc aaatatcagt ttgattgacc agtaagtctg tagggtaatc agttagttac
     1981 agcgcagcca cacactcgcg cgcaccgcgt tcgcacagct gctgatatgc cgccgtcacc
     2041 gcgccaacaa agtcggcatt ctgcggcaga tcatcggcaa aaataccgct caggcccagc
     2101 agcgctttca cgcggtctgc gccctgatac tgcgcgttaa tcttctggaa ctcggccagc
     2161 atcgggtcga ccacgtcaat ggcattaccc tgctcatcca cgccctgggt gtaacgcatc
     2221 cagccagcca cgcccagcgc caggtgacgc cagctgccgc cgttttgcag gtgcagacgc
     2281 accggatcca gcagacgctg cggtaacttc tggctgccgt ccatcgcaat ctgccaggta
     2341 cggtgacgca gagacgggtt gctgaaacgc tcgatcagca gcgtcgcata ggcgttcagg
     2401 tccgtacctt ccggcatcga cagcgttggc gcttgttcct gcatcatcag ggcaaaggcc
     2461 gctttgcgat aatccgggtt agtcatggtg tcggcaatag tttcatagcc gccgaggtaa
     2521 ccgaggtatg ccaggaaaga gtggctgccg ttcagcatac gcagcttcat catttcgaac
     2581 ggcacaacgt ctgcaacgaa ctgtgcgccc actttatccc agtccggacg accattaacg
     2641 aagttatctt caatcaccca ctgacggaac ggttcgcagg caatggcgca cgggtcataa
     2701 acacccagct ggtcagcaat ttcctgtaag gtttctggcg tcgccgccgg aacgatgcgg
     2761 tcaaccatgg tgcacgggaa ggtgacgttc tcttcaatcc atgccgccag ctgcgggtca
     2821 cgcgcctgag ccagccccag taccgcgacc ttcgccacat gaccgttttc acgcacgtta
     2881 tcgcaggaca tcaccgtaaa cgctttcagc cctttttcac gacgcagacg cagggcttcg
     2941 acgatgtaac caatcgcaga cttcggcgca gtcgggtttt ccagatcgtg cttgatcagc
     3001 gggttattga gatccaactg accgcttgcc gcatcagcgc agtagccttt ttccgtgacc
     3061 gtcagagaga caatcgccgt ctgcggacgc gccatcgcgt tgagaatacc ttcgcagccg
     3121 tcgatttccg gatgcagcgc ttctttcatc gaaccgataa ttttcagttc ggtgctttcc
     3181 gcgccttttt ccgccacggt gtacagcagt tgctgttttt tcaggttttc aatcagcaca
     3241 cggtcgttac ctggcatcag gttaacttcg cagatacccc agtcgctgtc ggtgctttcc
     3301 agcagatgat gggtatacag cgcctggtgc gcgcggtgaa acgccccgca accgagatgc
     3361 acaatgcgtg attccagacg agaatgcacc catgacgggc gggcaaccgg cagattgctg
     3421 tcaacaatag tagtcatcgt aaactccaga atccgtcttc agagagtgac ggcaaggaag
     3481 aaggaaccgg cacggcgcgc agccatgccg gtggatatta acggctaaag aaagcgcgct
     3541 ggatcgccag ttcgacaccg cgaacttcgg ccaggccttt cagacgacca attgcggagt
     3601 aacctgggtt agttttcttc ttcaggtcgt ccagcatctg atgaccgtgg tccggacgca
     3661 tcgggatcag gtcttctttg ccttccgctt tacgacggtg ttcctcttca acaatcgctt
     3721 tcaccacttc gtacatatca acgtcaccgt tcaggtgcgc cgcttcgtgg aaggttttcg
     3781 ggttatcttc acgcatggtg gagcgcagat gggtgaagta aatacgcgga ccgaactgct
     3841 tgatcatatc aaccagatcg ttgtcagcac gcacgccgta ggaaccggtg cacatggtga
     3901 aaccgttcgc catgctgttt acggtatcaa ccatccactg catatcttca atggtggaaa
     3961 caatgcgcgg caggccgagg atcgggcgcg gcggatcgtc cgggtgaaca gccatacgga
     4021 cgccaacttc ttcggcaacc ggaataatcg ctttcaggaa gacggcaaag ttttcgcgca
     4081 gtttggcttt gtcgatatct ttatatagct ccaggtgctt acggaactgg tcgagggtgt
     4141 acccttcttc cgcgcccgga agaccagcaa tgatgttacg ggtcagacgc gctttgtctt
     4201 cgtcgctcat agtggcgaag cgaacagctg cctgagcaat ttcttcttcg gtgtaatccg
     4261 cttccgcgcc tggacgtttc aggatatgca tttcgaatgc agcgaattcg atctggtcaa
     4321 agcgcagagc tttggagccg tctggcagca cgtattcgag gtcagtacgg gtccagtcga
     4381 gcaccggcat gaagttgtag cacacggtgc gaatgccgca ctgcgccagg ttgcgcagag
     4441 tctgctgata gttagcgatc cactgctcat agttgccagt gtgggttttg atatcttcgt
     4501 gaattggcac gctttctacg acagaccata ccaggcctgc gtcttcaacg atcgccttgc
     4561 gtttgaggat ctcttcaacg gaccatactt cgccgttcgg gatatggtgc agcgcggtaa
     4621 ccacgccagt tgcgcccgcc tgacggacat cagctaaaga aaccggatcg tttgggccgt
     4681 accagcgcca ggtctgttcc atgatttttc ctctttcaca tagtgggttc cacagcaaag
     4741 agacgcggaa ccctggagtt gtttaggtat ccggatgaac aaccagaccg cagccgtggc
     4801 gttgcatcgg atgagatggc gtataagttc taccttttgg taatacatgt caacccgaat
     4861 tctaaaattg gttaaccaca tcacaagaat ttcactctcc tggttgttgc tgcaaattgc
     4921 agcaaagggt aatcgatggt tattgatcag aggatgtgac attcatcgca acaatggttg
     4981 accaatttac ataacatgtc gcccaaaata acaacggttc aaccaggtaa ggtaaaacgt
     5041 tatgcatgtg cttaacattc tctgggtggt attcggcatt ggtctgatgc tggtactgaa
     5101 tttgaagttc aaaatcaatt caatggtggc tttgttggtg gcggcgctgt ccgtcgggat
     5161 gctggcgggc atggatttga tgtcgctgct gcacaccatg aaagcgggct tcggcaacac
     5221 gctgggggaa ctggctatca tcgtggtgtt cggtgcggtc atcggtaaat tgatggtcga
     5281 ctccggcgcg gctcaccaga tagcacatac gctgctggcg cgtctcggtc tgcgctatgt
     5341 acagctgtcg gtgattatca tcggcctgat ttttggtctg gcgatgttct atgaagtggc
     5401 ctttatcatg ttagcgccgc tggttattgt cattgccgcc gaagctaaaa ttccgttcct
     5461 gaaactggcg atcccggcag tagcagctgc cactaccgca cattcactgt tcccaccgca
     5521 gccgggtcct gtggcgctgg tgaatgctta tggcgcagat atggggatgg tttatatcta
     5581 tggcgtactg gtgacgatcc caagtgtaat ctgcgcaggt ctgatcctgc cgaagttcct
     5641 cggcaatctt gagcgcccaa cgccatcatt cctgaaagca gatcaaccgg tagatatgaa
     5701 caatctgccc tctttcggcg tttcgattct ggtgccgctg atcccggcga tcattatgat
     5761 ctccaccacc atcgccaata tctggctggt aaaagatacc cctgcctggg aagtggttaa
     5821 ctttatcggt tcctcgccga ttgcaatgtt tattgcgatg gtggttgcat tcgtactctt
     5881 tggcaccgca cgtggtcatg acatgcagtg ggtgatgaac gcttttgaaa gtgcggtgaa
     5941 aagcattgca atggtgattc tgatcatcgg tgcgggtggc gtgttgaagc aaactatcat
     6001 cgacaccggc attggcgaca ccatcggcat gttgatgtcc cacggcaata tctcgcccta
     6061 cattatggca tggctgatca ctgtgctaat tcgtctggcg acgggtcagg gtgtcgtttc
     6121 ggcgatgacc gccgccggga ttatcagtgc tgcaatcctt gatccagcaa ccggtcagct
     6181 ggttggcgtg aatccggcgc tgctggtgct ggcgacggct gcgggttcca acaccctcac
     6241 ccacattaat gatgcctcat tctggctgtt caaaggttac tttgacctgt cggtaaaaga
     6301 cacgttgaaa acctggggac tgctggagct ggtcaactcc gtggttgggc tgattattgt
     6361 gttgattatc agcatggtag cgtaaaagaa caccagagcc tgccaatggc gggctctgtt
     6421 cggattgtcg gcaaagtgcg ctttgtttat gccagatgcg acgctaacgc gtcttatctg
     6481 gcctacaaag ggctaacgtg caggttttta gcttcaggta atattgcgta cctgcattag
     6541 caatgccctg tgatttcttt attgataaac aaaagtcacg ccaataatcg attgcacatt
     6601 ccctgcagtc acctgccctc cggtacgtgc gtaatttgcc gttaatccca gacttaccgc
     6661 cgaagtccct actgctccta acgataccgt gttattcgct ggaataatcg taccgttgcg
     6721 cgtcaactgt acgccgacgc cctgcgctag tgaaaacgac gcggtattgg tgaaaatcga
     6781 gttgcccgca tctgcggttg tgccggagag gtaatacccc aggttttggc ttttcgcaca
     6841 ataaacggta agaggaattg gcactgaacc agggtagtcc ggcagagtaa cggtgacatc
     6901 acgagcagaa acatcgcagc cgccagtagg caccaccaca tcattattgg cgtaaatatt
     6961 ccacacaaac tggaaatcat cgctgttata gttgttggtc tgtcgcaaaa taagcacggc
     7021 aattaatgag ccagctttaa tcgccacccc gcccgcactg ctcacaggcg tcaaataaag
     7081 cgccaccggc cacggcttat ccgttctcga attataaaca acccgcggcg tttcgctggt
     7141 ggtcggaaat ggatagctac tgccactata ttttacggtc ccggaaaaat tagataacac
     7201 gccgccataa gccgagcctc gttgcagtgt gacatagtct gtaatggttt ccggataatc
     7261 gttatggcaa aagatttgcg tcgaaagatc tacgaccagg ttttgcccca cattcacgac
     7321 aggcgcaagg tttacataaa cattagcgct gccaccacca atagggatag cggtaccatt
     7381 ggcggtttta caggcgaatg accaggcatt taccgaccag cccatcagca gtacagcaaa
     7441 cagggtaata actcgtttca ttacaatcat ctcttcgggt tcagctgtag gtataggtga
     7501 tgctaatcac tgcctgaatg gttccctgag tggttccgcc atttaccgtt aatgctctga
     7561 cctgtaacgg gaagtgcgct gattgtgagg aatcatccac ctgaactgtt ttggttgcgc
     7621 cagtattcaa tgtgttgcca ctgtcatcct gtagctctaa ctggatgttt tgcgcggtcc
     7681 cctggttttt ataatatccg gtactgtcgg ctgccccgct gaagctggca gtgaccctcg
     7741 acgttcccac cggacaatta gtcaactcaa gcgcaacatc atgccaggcc gatgccgccc
     7801 cggcagacat cagactgaaa gaataaagat cgccgagatc aaccgtggca ttggtggtgg
     7861 aaactgtgca cggtttggcg acgaccttac cgttcaccgt gatggtgaca tcggctgcct
     7921 gtatcgtcgc acttgcgagc gccaatattg ccgccaatac atacccacgt ttgcaccatt
     7981 tcatgagcat ctccagttac tgatattcaa gagtgaaggt agccgtggca ttgatatgcc
     8041 ccgcagtgac aggcacctgt gtcgccatta gccgggcgta aaaattcagc gtatttggtt
     8101 tacccggcgt cagggtcgtc cacgaaatcg cggacgatgg agcattaagg ggtatctgat
     8161 tttgctgctc attcagaagc tgtattccca gtcccgaagc cgctgacacc gtattttcaa
     8221 gtgcaagcag gttggcattg tggctatctg caacgccggt aaacccaact tttacggcag
     8281 aaacggcatt accacagggt gacagcaaaa tacgaaatgg aacgacagga gtcgtcgcgc
     8341 caatgttgtt aaattgcttc gccgcgtttt ccatcagatc aacagtaaaa ttggttgatt
     8401 cagcggccac actacagccg ttatctctga catagccgcg gatagtaatc gtgctatccg
     8461 cagccaaagc gtgactcacc gccagccaca aaaaagcgca cagaagataa aaaggtttgt
     8521 ttctcatcac gcccccttaa cgacattcag ctgatagctg ggttaataac tgctgctgac
     8581 tctctggtgg cagttgataa ttggcgacac agtgagcatt ttcctcttct ccccatttca
     8641 cctgaacttt tcccgctaaa ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa
     8701 tgccgctact ctggctactc tctgatgtca ccatcgcccc gaacggcagc ggcttattat
     8761 tgtgggttag cgtcatgagc agttttatcc caacgcgcgc tttaaactct gctcgcacga
     8821 tcgccccacg agtgggaaca acgttagcga ccgcgttatc taaatcgacg ttatcagcca
     8881 gggtattggt atccagcgcc actctatttt cccgatattc agtggcataa ggcagcacgg
     8941 cataaccgcg ccagtcggta cgcacccccg tctggttttc gacttttgca tcttttgcgc
     9001 caggcgcttt aacaagcacc accgtatcgt ttaacggctg ccccagcgtt acgccattgg
     9061 catgagccag taccccaccg ctgactccgt aatagagctg cttaatatca tcgctatggc
     9121 tgtaaccgat attggcattg ccgtaaccac cgcgataatt cagcgtggcg tagcctgtac
     9181 ttccgctatt accatcgcct cccccggcat agccggtttg cacgctatag ctgaggttgt
     9241 tgtcttccag caacgtaccg tatacaccag ccagattgtt cattcgaccg ttgagatcgt
     9301 gtgacatgct gtagctggca ctggcatgtc gccactgaga tttactgtca gaacgcagcc
     9361 agtggctgaa aggaatattg acgttaagcg ctaacatctg atcccgtcct ttttgccagg
     9421 cgtttttcgt caggctatag ctgagcgtcc agttgatatc ttcgaacgca gtatttaatc
     9481 cagcctggaa ttgctcatcg acattactcg ttccccaata agtttgatgg ctaccactca
     9541 aatacagtgt tgatgtgcgc ccgagttgct gagtaacggt gagctgtaat ttcccgcgtt
     9601 tgttataagc gaggttgtaa tagtcggtga atttcggctt aacctgaata actccatcct
     9661 gtgtttcgat gttgtagcca ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc
     9721 cgctggtcga ataacggtaa cccactaact gaatattcgt gcctgactca ttgagcgatt
     9781 tgttatagag aaaacgcacc gattgtccgt catgctgact gtcatcggga agtgtagaat
     9841 tagcctgcgt catatccgca gacagagcac ccaacgcccc catgtttttc ccgataccga
     9901 aattaaaagc acgataacga tccgccagtt gcgttccacc atatattgtc cagccagccg
     9961 gaaggccgtg gagtaatgta ctctggaaaa agcggggttt ttcctgctgc gcatttccac
    10021 tacggtattc tcctgccgta atggaataac gagtatgccc ttcacgttgc aaaagcggga
    10081 ctgacgaata gggtacggta aaaatctgcg tgctgccgtc agcctcttta atcgttacct
    10141 gcaagtcacc actattacct gcagcataga tatcgttgat ggtaaaaggc cccggcggca
    10201 ccgtactatt ataaatgtca tacccatttt gtttaatagt gacctgtgca gtaccacgag
    10261 caataccgtg gatcaccggg gcaaatcctc tttggctatc gggtaacata ttgtcatctg
    10321 aggccaattg tgcgccgcga aagttaatac catcgaaaat atcgccctga gtataaccat
    10381 cacccagcgt cagccgggaa cgtaacggta ttatgtctcg ctcaagccag gtattgatat
    10441 actgccattt atttttgcta cctgatgatc tgtcgctact gttataactc caggtggtat
    10501 tgtcgcgtaa acgccacgca ccaatattta acccactctg taggtttaaa tatgcataat
    10561 ggctgttacc cccaatccga ttctgtacac tatttccgct gaaattataa ttgagcaatc
    10621 cggaattaat accaggatcc cataactcag gaggaatata accacgcgcg cgattactca
    10681 taaatgcctg agggatcgtc aggttgagtc gctgctgacc aacatccaga tgcgcagtag
    10741 cgtcatggat cattgaggtt aatggcacgc aggcatcatc cgccagcaga ttcataccgg
    10801 agacagaagc cgtattcagc cccatacttg caagttgcgc gcgtgtcagg cagggaacaa
    10861 tcccttgttc actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat
    10921 tcaaatagat atcgacacga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg
    10981 ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg cggattaaaa tagagttcgg
    11041 cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac aaagagccgg acaaaaaaac
    11101 cagccaaacg atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat
    11161 tcagatatga cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag
    11221 gcgaaaaatt ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat
    11281 aatcatttat tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc
    11341 tttcgcccat tggaggcacc aatgcatttt caagaacccg ggttccggca ttcaactctg
    11401 ttaccgtcag gtaatagggt gtcgggttaa tcagagtcag agaattcgcg ctacgacgaa
    11461 atcttaattt ttctgcgacc tgatcgggtg gcaacgctaa tttagccggg cgatagtaca
    11521 gtttaatgcg gctgataatt gcgagctgta gcgtattctc agtcaatttt gatttatcca
    11581 ttgacggaat cgctttaacg ttcatccaga ataaactttc ccgatcctgt ggcaattggt
    11641 tatttgttgc atcaagaata cgtaaggtat tctctttttt tcccttcatc gcaaacagag
    11701 gtggcgtcac gataaaacga ccatccttta caccatcggc attttccacc catgattgaa
    11761 ttaaataggt gctattttca tcattatttg tcacggcaag ttgcacttgt ttttgccctg
    11821 ccggataaat tacgcgggtc gcacctaagg ccactcccgc ttcagcgcgt ccggcaacca
    11881 tcattgccat gaacatcagg atacctgcca gcaagcagaa tgttatttcc tgcgatttcc
    11941 ttacattgac gtttttatta ctcactgtcc tgttcctgtt atcacattat ctgctgaaca
    12001 attactgata ggttaaagag aaccaggcct gggcattggc gatgccgcca gtaacccgac
    12061 gcccggtagc acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt
    12121 tccagtttgc tggaggacga ttaatcggta cgaggtttcc ttcatcatca aataacgcta
    12181 cgccaatgtt tgtggctatc cctggcccct ctcccacgga aagcacatcc ggatttttac
    12241 catccgcgac accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc
    12301 gtaaatgaat aacaaaaggc accggtgcgc tatcttcccc taccgcatga aaccggttac
    12361 tgctgatttg ccccatatta accgtcattt gtttatctcc ggcttcaatc cggcaagttt
    12421 ccgcaataat gacgccctga aattgcatat ttccgccggg caacgtggta ttccatttat
    12481 ttccggccag agcaaacatt ggcagcaacg acgctaacag aaataatctc ttccttttca
    12541 tcgttatttt tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ctgaacctgg
    12601 gtaggttatt gatactgaac cttgaaggtc gcatccgcat tagcagcacc cggggttgcc
    12661 tcgccgattg cataataacg cgcctggaac ggaatggtgt tggtaccgtt attcaaggtt
    12721 gtttgtacac taaatgtcgc accgtccagc gtcagctcag cacccgttct gtccagtatc
    12781 tgcacaccaa catttgttgc gctacccgca gctgaactct gcagagccag tactttagga
    12841 tgagcactgt caatcgcagt ccccaagaag gcaacagcgg cttttgtggc aacactggta
    12901 tcgcaatcat tcagctgaat gttaaaaccg acagcagagc tggttgctcc agtctgcttc
    12961 agactagcgg tacgaacctg gcccaactga acggtttgat caacagagcc tgcatcaact
    13021 gcgcaagcgg cgttaacaac ttccccttta aagtgaacgg tcccaccatt taccgtcgtg
    13081 ggtgtagttt cagccagagc cgctgtagaa ctcagggaca gagccgacag aacaacaatt
    13141 gccagagttt taattttcat actgctttcc tttcaaaaaa ctatttctaa atcgacatgg
    13201 gcagtcgttc tgtacacttt gttttgtcaa cgagtttgac tgccaacact gcacagtttc
    13261 cccccaaaag atgaaacatt tggggccatt ttgactcata gaggaaagca tcgcgcacaa
    13321 actttttcag tttatttgtt ggcttgatat tctattgtta tctttattta tagattttta
    13381 tattgcatga ggtg
//
LOCUS       DAOFQD010000122        13391 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.122, whole genome shotgun sequence.
ACCESSION   DAOFQD010000122 DAOFQD010000000
VERSION     DAOFQD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.122"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            86..1195
                     /gene="livK"
                     /locus_tag="QBF46_003750"
     CDS             86..1195
                     /gene="livK"
                     /locus_tag="QBF46_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDQ6675878.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1243..2169
                     /gene="livH"
                     /locus_tag="QBF46_003751"
     CDS             1243..2169
                     /gene="livH"
                     /locus_tag="QBF46_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDQ6675879.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2166..3443
                     /gene="livM"
                     /locus_tag="QBF46_003752"
     CDS             2166..3443
                     /gene="livM"
                     /locus_tag="QBF46_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDQ6675880.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3440..4207
                     /gene="livG"
                     /locus_tag="QBF46_003753"
     CDS             3440..4207
                     /gene="livG"
                     /locus_tag="QBF46_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDQ6675881.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4209..4922
                     /gene="livF"
                     /locus_tag="QBF46_003754"
     CDS             4209..4922
                     /gene="livF"
                     /locus_tag="QBF46_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDQ6675882.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPCATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5319..6635
                     /gene="ugpB"
                     /locus_tag="QBF46_003755"
     CDS             5319..6635
                     /gene="ugpB"
                     /locus_tag="QBF46_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDQ6675883.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6733..7620
                     /gene="ugpA"
                     /locus_tag="QBF46_003756"
     CDS             6733..7620
                     /gene="ugpA"
                     /locus_tag="QBF46_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDQ6675884.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7617..8462
                     /gene="ugpE"
                     /locus_tag="QBF46_003757"
     CDS             7617..8462
                     /gene="ugpE"
                     /locus_tag="QBF46_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDQ6675885.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8464..9534
                     /gene="ugpC"
                     /locus_tag="QBF46_003758"
     CDS             8464..9534
                     /gene="ugpC"
                     /locus_tag="QBF46_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDQ6675886.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPMVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            9531..10274
                     /gene="ugpQ"
                     /locus_tag="QBF46_003759"
     CDS             9531..10274
                     /gene="ugpQ"
                     /locus_tag="QBF46_003759"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDQ6675887.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10261..10701)
                     /locus_tag="QBF46_003760"
     CDS             complement(10261..10701)
                     /locus_tag="QBF46_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDQ6675888.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10821..12563
                     /gene="ggt"
                     /locus_tag="QBF46_003761"
     CDS             10821..12563
                     /gene="ggt"
                     /locus_tag="QBF46_003761"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDQ6675889.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGIVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12601..12885)
                     /locus_tag="QBF46_003762"
     CDS             complement(12601..12885)
                     /locus_tag="QBF46_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HDQ6675890.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(13085..13240)
                     /locus_tag="QBF46_003763"
     CDS             complement(13085..13240)
                     /locus_tag="QBF46_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675891.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT         3001 a         3378 c         3801 g         3211 t
ORIGIN      
        1 acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca aagcaacaca
       61 acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca tcgcagggat
      121 gattgcactg gcaatttcac acaccgcttt agccgacgat attaaagttg ccgttgtcgg
      181 cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg cgcgtcaggc
      241 gattaaagac attaatgcca aagggggaat taaaggcgat aagctggttg gcgtggaata
      301 tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg ttaatgacgg
      361 cattaaatac gttattggtc atctgtgttc ttcttctacc cagcctgcgt cagatatcta
      421 tgaagacgaa ggtattctga tgatctcgcc gggggcgacc aacccggagc tgacccaacg
      481 cggttatcaa cacatcatgc gtactgccgg gctggactct tcccaggggc caacggcggc
      541 aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg acaaacaaca
      601 atatggcgaa gggctggcac gttcggtgca ggacgggctg aaagctgcta acgccaatgt
      661 tgttttcttc gacggcatta ccgccgggga gaaagatttc tccgcgctga tcgcccgcct
      721 gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa tggggcagat
      781 gctgcgccag gcccgttccg ttggcctgaa aacccagttt atggggccgg aaggtgtggg
      841 taatgcgtcg ctgtcgaata ttgctggcga tgctgccgaa ggcatgttgg tcaccatgcc
      901 aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga aagcagacaa
      961 gaaagatccg tccgggcctt atgtctggat cacttacgcg gcggtgcaat ctctggcaac
     1021 agcccttgag cgtaccggca gcgatgagcc gctggcgctg gtgaaagatt taaaagcaaa
     1081 cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc ttaagggatt
     1141 tgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca agtgatcatc
     1201 ccaccgcccg tgaaaaacgg gcgggtttag aaaggttacc ttatgtctga gcagttttta
     1261 tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacctacgc gctgattgcc
     1321 atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca cggcgaggtt
     1381 tacatgatcg gcagctacgt ctcatttatg atcatcgccg cgctgatgat gatgggcatt
     1441 gataccggct ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat tgccagcgcc
     1501 tacggctgga gtatcgaacg ggtagcctat cgcccggtgc gtaactctaa gcgcctgatt
     1561 gcgctcatct cggcaatcgg tatgtccatc ttcctgcaaa actacgttag cctgactgaa
     1621 ggttcgcgtg acgtggcgct gcccagtctg tttaacggtc agtgggtggt ggggcatagc
     1681 gaaaacttct ctgcctctat taccaccatg caggcggtga tctggattgt taccttcctc
     1741 gccatgctgg cgctgacgat tttcattcgc tattcccgca tgggtcgcgc ctgtcgtgcc
     1801 tgcgccgaag atctgaaaat ggcgagtctg cttggcatta acaccgaccg ggtgattgcg
     1861 ctgacctttg tgattggcgc agcgatggcg gcggtggcgg gcgtactgct cggtcagttc
     1921 tacggcgtca ttaaccccta tatcggcttt atggccggga tgaaagcctt taccgctgcg
     1981 gtgctcggtg ggattggcag cattccaggg gcaatgattg gcgggctgat cctggggatt
     2041 gcggaggcgc tctcttctgc ctatctgagt acggaatata aagatgtggt ctcattcgcc
     2101 ctgctgattc tggtgctgct ggtgatgccg accggtattc tggggcgccc ggaggtagag
     2161 aaagtatgaa accgatgcat attgcaatgg cactgctctc tgccgcgatg ttctttgtcc
     2221 tcgctggcgt ctttatgggc gtgcaactgg agctggatgg caccaaactg gtggtcgaca
     2281 gcgcttcgga tgtccgttgg cagtgggtgt ttatcggcac ggcggtggtc tttttcttcc
     2341 agcttttgcg acctgctttc cagaaagggc taaaaagcgt ttccgggccg aagtttattc
     2401 tgcctgcgat tgatgggtcc acggtgaagc agaaactgtt cctcgtggcg ctgctggtgc
     2461 ttgcggtggc gtggccgttt atggtgtcac gtgggacggt ggatatcgcc actctgacca
     2521 tgatctacat tatcctcggt ctcgggctga atgtggttgt tggtctttct ggtctgctgg
     2581 tgctggggta cggcggtttt tacgccatcg gcgcttacac ctttgcgctg ctcaatcact
     2641 attacggctt gggcttctgg acttgtctgc cgattgccgg gttaatggca gcggcggcgg
     2701 gcttcctgct cggctttccg gtgctgcgtc tgcgtggtga ttatctggcg atcgttaccc
     2761 tcggcttcgg cgaaattgtg cgcatattgc tgctcaataa caccgaaatt accggcggcc
     2821 cgaacggaat cagtcagatc ccgaaaccga ctttcttcgg ccttgagttc agccgtaccg
     2881 cccgtgaagg cggttgggac acgttcagta atttctttgg cctgaaatac gatccctccg
     2941 atcgcgtcat cttcctgtat ctggtggcgt tgctgctggt ggtactaagc ctgtttgtca
     3001 ttaaccgcct gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt gaagatgaga
     3061 tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgaccgct tttaccatca
     3121 gtgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgccagggc tttgtcagcc
     3181 cggaatcctt cacctttgct gaatcggcgt ttgtactggc gatagtggtg ctcggcggta
     3241 tgggatcgca gtttgcggtg attctggcgg caattttgct ggtggtatcg cgcgaattga
     3301 tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg ctgatgatga
     3361 tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg aaaaacggcg
     3421 cagcgaaagg agagcaggca tgagtcagcc attattatct gtgaacggcc tgatgatgcg
     3481 cttcggcggc ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc aggagatcgt
     3541 ctcgttaatc ggccctaacg gtgccggaaa aaccacggtt tttaactgtc tgaccggatt
     3601 ctacaaaccc accggcggca ccattttact gcgcaatcag cacctggaag gtttaccggg
     3661 gcagcaaatc gcccgcatgg gcgtggtgcg caccttccag catgtgcgtc tgttccgtga
     3721 aatgacggtg attgaaaacc tgctggtggc gcagcatcaa caactgaaaa ccgggctgtt
     3781 ctctggcctg ttgaaaacgc cttctttccg tcgcgcccag agcgaagcgc tcgaccgcgc
     3841 cgccacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg cgagtaacct
     3901 ggcctatggt gatcaacgcc gtctggagat tgcccgctgc atggtgacgc agccggagat
     3961 tttaatgctc gacgaaccgg cggcaggtct taacccaaaa gaaaccaaag agctggatga
     4021 gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg aacacgatat
     4081 gaagctggtg atgggaattt cggaccgaat ttacgtggtc aatcagggga cgccgctggc
     4141 aaacggtacg ccggagcaaa tccgtaataa cccggacgtg atccgtgcct atttaggtga
     4201 ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac tacggcaaaa
     4261 tccaggcgct gcatgaggtg agcttgcata tcaatcaggg cgagattgtc acgctgattg
     4321 gcgcgaacgg ggcggggaaa accaccttgc ttggtacgtt atgcggcgat ccgtgcgcca
     4381 ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg gcgaaaatca
     4441 tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg atgacggtgg
     4501 aagagaacct ggcgatgggc ggcttctttg ccgaacgtga ccagtttcag gagcgcataa
     4561 agtgggtgta tgagctgttt ccacgtcttc atgagcgtcg tgttcagcgg gcgggcacca
     4621 tgtccggcgg tgaacagcag atgctggcga ttggtcgcgc gctgatgagc aacccgcgtt
     4681 tgttgttgct tgatgagcca tcgctcggtc ttgcgccgat tatcatccag caaattttcg
     4741 acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag cagaacgcca
     4801 accaggcgct aaagctggcg gatcgcggct acgtgctgga aaacggccat gtggtgctct
     4861 ccgataccgg cgacgcgctg ctggcgaacg aagcagtgag aagtgcgtat ttaggcgggt
     4921 aataacacgt tgattgataa ggagtcaaaa gactcctttg agacaggtga caaatgtaaa
     4981 attgcctgat gcgctgcgct tatcaggcct actgggtgag ttgcaatata ttgaatttgc
     5041 acgatcttgt aggccggata aggcgtttac gccgcatctg gcatgaaacg atgagtaatc
     5101 tgtagagttt gattcagacc ttctattttt ccgcttatcc gtgccccatc tcccattttc
     5161 cctcacccac gccgtcaccg ccttgtcatc tttctgacac cttactatct tacaaatgta
     5221 acaaaaaagt tatttttctg taattcgagc atgtcatgtt accccgcgag cataaaacgc
     5281 gtgaattcgc gcattcggta caacaagaga gataaacgat gaaaccgtta cattatacag
     5341 cttcagcact ggcgctcgga ctggcgttaa tggggaatgc acaggcagtg acgaccattc
     5401 cgttctggca ttctatggaa ggggaactgg gtaaagaggt ggattctctg gcccaacgtt
     5461 ttaacgccga aaatccggat tacaaaattg taccgaccta taaaggcaac tacgaacaga
     5521 atttaagcgc ggggattgcc gcatttcgta ccggcaacgc tccggctatt ttgcaggttt
     5581 atgaagttgg caccgccacc atgatggcat cgaaagccat taaaccggta tatgacgtgt
     5641 ttaaagaggc ggggattcaa ttcgatgagt cgcagtttgt gccgacggtt tccggttact
     5701 actccgacag caaaacgggc cacttactct cccagccatt caacagctcg acccccgttc
     5761 tctattacaa caaagacgcc ttcaagaaag caggattaga cccggaacaa ccgccgaaaa
     5821 cctggcagga tctggcggac tatgccgcga aactgaaagc ctccggtatg aagtgcggct
     5881 acgccagcgg ctggcagggc tggatccaac tggaaaactt tagcgcctgg aacggtctgc
     5941 cgtttgccag caaaaacaac ggctttgacg gcacggacgc ggtgctggag ttcaacaagc
     6001 cggagcaggt gaaacacatc gccatgctcg aggagatgaa caagaagggc gacttcagct
     6061 acgtcggtcg taaggatgaa tccaccgaga agttctataa cggtgattgc gcgatgacca
     6121 ccgcctcttc cggttctctt gccaacattc gcgagtacgc caaatttaac tacggcgtag
     6181 gcatgatgcc ttacgacgcc gatgcgaaag atgcgccaca aaacgccatt atcggcggag
     6241 ccagcctgtg ggtgatgcag ggtaaagata aagaaacgta taccggtgtg gcgaagttcc
     6301 tcgatttcct cgcgaagcca gaaaacgctg ccgagtggca tcagaaaacc ggttatctgc
     6361 caatcaccaa agcagcgtat gacctgaccc gtgagcaggg cttttatgag aaaaacccag
     6421 gggcggatac cgcgacgcgt cagatgctga ataagccgcc gttgccgttc accaaagggc
     6481 tgcgtctggg caacatgccg cagatccgcg tgattgtgga tgaagagctg gagagcgtgt
     6541 ggaccggtaa gaagacacca cagcaggcac tggataccgc cgttgagcgt ggaaatcagt
     6601 tgctgcgccg ctttgagaaa tcgacgaagt cttaatcagt gtaatgtcgg atgcgtttcg
     6661 cttatctgac ctggcatcgc gtgtaggccg gataagcgaa gcgcatccgg cacagttcag
     6721 gaattaaccg taatgtcatc atcccgtccg gtgttccgct cgcgctggct gccttatctg
     6781 ctggtcgcgc cgcagctcat catcaccgtt atctttttta tctggcctgc gggcgaagcg
     6841 ttgtggtact cgctacaaag cgtcgatccg tttggtttct ccagccagtt tgtcggcctg
     6901 gataacttcg tcacgctgtt ccatgacagc tactatctcg actccttctg gacgacgata
     6961 aaattcagca cctttgtcac cgtcagcggt ttgctggtat cgctgttctt tgcggcgctg
     7021 gtggagtaca tcgtgcgcgg cagccgtttc tatcaaacct taatgttgct gccttacgcc
     7081 gtggctcccg ccgttgccgc cgtattgtgg atcttcctgt ttaaccccgg tcgcgggctg
     7141 atcacccatt ttctcgcgga gttcggctac gactggaacc acgcgcaaaa cagcggtcag
     7201 gcaatgtttc tggtggtgtt tgcctcagta tggaagcaaa tcagctacaa cttcctgttc
     7261 ttctatgccg cgctgcaatc cattccccgt tcgttgatcg aagccgcagc catcgacggt
     7321 gcagggccga ttcgccgctt ctttaagatt gcgctgccgc ttatcgcccc ggtgagtttc
     7381 ttcctgctgg tagtgaacct ggtgtatgcc ttcttcgaca ccttcccggt gatcgacgcc
     7441 gccacgtccg gcgggccggt tcaggccacc acgacgctga tttataagat ctaccgcgaa
     7501 ggttttaccg gactggatct ggcttcgtct gccgcacagt cggtggtgtt gatgttcctc
     7561 gtcatcgtgc tgacggtggt gcagttccgc tatgttgaaa gcaaggtgcg ttaccaatga
     7621 ttgagaaccg tccgtggctg acgatattca gccataccat gctgatcctc gggatcgcgg
     7681 tgatcctctt cccgctgtac gtggcgtttg tcgcggcgac gctggataaa caggccgtct
     7741 atgccgcgcc gatgacgctc atccccggca cacatctgct ggaaaacatc cacaacatct
     7801 gggtgaacgg ggtaggcacg aatagcgcgc cgttctggcg gatgttgctt aacagctttg
     7861 tgatggcgtt cagcattacg ctcggcaaaa ttaccgtctc gatgctctcg gcatttgcca
     7921 ttgtctggtt tcgttttccg ctacgtaacc tcttcttctg gatgattttt atcaccctga
     7981 tgctgccggt tgaagtacgt atcttcccga cggtggaagt catcgccaac ctgcagatgc
     8041 tcgacagcta cgccggttta acgctgccgc tgatggcctc ggcgaccgct actttcctgt
     8101 tccgccagtt ctttatgacg ctgccggatg agctggtgga agccgcgcgg atcgacggcg
     8161 catcgccaat gcgcttcttt tgcgacatcg tttttccgct ctccaaaact aatctggcgg
     8221 cgctgtttgt gatcaccttt atctacggct ggaatcagta tttgtggccg ttgttgatta
     8281 ttaccgatgt ggatctcggc accaccgtgg cagggatcaa agggatgatc gctacaggcg
     8341 aaggcaccac ggaatggaac tcagtgatgg cggcgatgtt gttaacgctt atccctccgg
     8401 tggtgattgt tttagtgatg cagcgtgcct tcgtgcgcgg cctggtcgat agtgagaaat
     8461 aagatggcag gactgaaatt acaggcagta accaaaagct gggatggtaa aacccaggtc
     8521 attaaaccgc tgacccttga tgtggcggat ggcgaattta tcgtgatggt cgggccgtct
     8581 ggctgcggga aatcgacgct gctgcgcatg gttgccgggc tggagcgggt gacagaaggc
     8641 gatatctgga tcaacgacca gcgcgtgact gaaatggagc caaaagatcg cgggattgcg
     8701 atggtgttcc agaactacgc gctttatccg catatgagtg tcgaagaaaa catggcgtgg
     8761 gggctgaaaa ttcgcggcat gggcaagcag caaattgccg agcgcgttaa agaagcggcg
     8821 cgcattctgg agctggacgg tctgctcaaa cgtcgcccgc gcgagctttc cggcggtcag
     8881 cgccagcgtg tggcgatggg ccgcgcgatt gtgcgcgatc cggcggtgtt cctgtttgat
     8941 gagccgctct ctaacctcga tgccaagctg cgcgtgcaga tgcgtcttga actgcaacag
     9001 ttgcaccgtc gcctgaaaac gacttcactc tacgttactc acgatcaggt tgaagcgatg
     9061 acgctcgccc agcgagtaat ggtgatgaac ggcggcgttg ccgaacagat tggcacacca
     9121 gttgaagtct acgaaaagcc cgccagcctg tttgtagcga gttttatcgg cagtccggcg
     9181 atgaacctgc tgacaggccg cgtgaataac gaaggcacgc atttcgaact ggacggcggt
     9241 attgagctgc cgctaaacgg tggctaccgt cagtatgccg ggcgtaaaat gactctcggc
     9301 attcgcccgg aacatattgc gctaagctcg caggcagaag gcggcgtacc gatggtgatg
     9361 gacacgctgg agatcctcgg cgcagataac ctggcgcacg gacgctgggg cgagcagaag
     9421 ctggtggtgc gactggcgca tcaggagcgc ccgacggcag gcagcacgct gtggctgcat
     9481 ctgccggaaa atcagctgca tctttttgat ggtgaaacag gacaacgagt atgagtaact
     9541 ggccttatcc ccgcatcgtc gctcatcgtg gcggcggtaa gctggccccg gaaaacaccc
     9601 tggcggcaat cgacgtcggg gcaaaatacg gtcataagat gatcgaattt gacgcgaagt
     9661 tatcgaaaga tggcgagatc ttcctgctcc atgacgacaa tctcgaacgc accagcaacg
     9721 gctggggcgt cgcgggtgaa ctgaactggc aggatttact gcgcgtggat gcgggcagtt
     9781 ggtacagcaa agcgtttaaa ggtgagccgc taccgttgct ttcgcaggtg gcggaacgct
     9841 gtcgtgaaca cgggatgatg gcgaatatcg aaatcaaacc caccactggc actggaccat
     9901 taacgggcaa aatggtggcg ctggcggcac gccaactgtg ggccggtatg acgccgccgc
     9961 tgctgtcatc gtttgagatt gatgctttag aagccgcaca acaggcagta ccggaactgc
    10021 cgcgcggttt gttgctggat gagtggcgcg acgactggcg cgaactgacc gcgcggctgg
    10081 gctgcgtctc tattcatctc aatcataagt tactcgataa agtgcgagtg atgcagttga
    10141 aagacgccgg actgcggatt ctggtttata ccgtcaataa accccagcgc gcggcagagt
    10201 tgctgcgctg gggcgtggat tgcatctgta ccgatgcgat tgacgtgatt ggtccgaact
    10261 ttacggccca atagttttca acggaatgtc aggctgcggc gtgctgggct gattcagcat
    10321 gtcgccgttt ctcctctccg gcagcatatg ctgctgattc aacgaactat ccggattacg
    10381 gttgctgttt aacatcccgc cgttggtgtt gggcagcatt tgctgccggg cgggatttcg
    10441 ctcccccggc tgtgactgca acacccgctg agaattattg tttatctggt tttctaaatg
    10501 ctgctgttgc aactgagttt gcgttttcag ttgctgattc agcatccctt tttgctggat
    10561 ttgctgagtc tgcatctggg tttgcatccg ctgctggctg ggtatctgat accccggctg
    10621 gttagggttg ttcagagtat taatgggctg tgcaaagccg acaaacggca ggagtgccgt
    10681 aagaatcaga agtcgtttca tcgcgtatcc tcctctgaag atatccttta agtttactcg
    10741 cttcccgaca aaacgatgat taattcagag ttatatacca ggcttagctg gggttgcccc
    10801 ttaatctctg gagaataacg atgataaaac cgacgttttt acgccgggtg gccattgctg
    10861 ctctgctctc aggaagttgt tttagcgccg ccgccgcgcc tcctgcgccg cccgtctcgt
    10921 atggtgtgga ggaagatgtc ttccacccgg tacgcgcgaa acagggaata gtagcgtctg
    10981 tggacgccac tgccactcag gtgggggtgg atatcctcaa ggagggcggg aatgccgttg
    11041 atgccgccgt ggcggtgggc tacgcgctgg cggtaacgca tccgcaggca gggaatctgg
    11101 gcggtggtgg ttttatgtta atccgctcga aaaatggcaa taccacggct atcgatttcc
    11161 gcgaaatggc acccgccaaa gcgacccgcg atatgttcct cgatgatcag ggcaacccgg
    11221 acagcaaaaa atcactcact tcgcatctgg cttccggcac accgggtacg gtagcaggtt
    11281 tctcgctggc gctggataaa tacggcacca tgccgctgaa caaagtcgtg cagcccgcgt
    11341 ttaaactggc acgcgatggt tttatcgtta acgacgcgct ggctgacgat ctcaaaacct
    11401 acggtagcga agtgttgccg aatcacgaaa acagtaaagc tatcttctgg aaagagggcg
    11461 agccgctgaa aaagggcgac acgctggtgc aggcgaacct ggcaaagagc ctggagatga
    11521 ttgctgaaaa cggcccggac gaattctata aaggcacgat tgcggaacag atcgcccagg
    11581 agatgcagaa aaacggtggc ttgatcacta aagaagattt agcggcttat aaagcggtcg
    11641 aacgcactcc gataagcggc gattatcgcg ggtatcaggt ttactccatg ccaccgccat
    11701 cctccggcgg gatccatatc gtacaaatcc tcaatattct ggaaaacttc gatatgaaga
    11761 aatacggctt tggcagcgcc gatgcgatgc aaatcatggc agaagcggag aaatacgcct
    11821 acgccgaccg ctcggaatat cttggcgacc cggattttgt caaagtcccg tggcaggcac
    11881 tgaccaataa agcctatgcc aaatccattg ccgatcaaat tgatatcaac aaagcgaagc
    11941 cgtccagtga gattcgccct ggcaagcttg cgccttatga gagtaatcaa actacccatt
    12001 actctgtagt ggataaagac ggtaacgcgg tggcggtgac ctatacgctg aacaccacct
    12061 tcggtacggg tattgtcgcg ggcgagagcg gtattctgct taataaccag atggatgatt
    12121 tctccgccaa accgggcgta ccgaacgttt acgggctggt gggcggtgat gccaacgccg
    12181 tcgggccgaa caaacgcccg ctgtcgtcga tgtcgccgac cattgtggtg aaagacggta
    12241 aaacctggct ggttaccggt agcccaggcg gtagccggat tatcaccacg gtgctgcaaa
    12301 tggtggtgaa tagcatcgat tatggcatga acgtcgccga agcgaccaat gcgccgcgtt
    12361 tccaccatca gtggttgccg gacgagctgc gtgtcgaaaa agggtttagc ccggatacgc
    12421 tcaagctgct ggaagcaaaa ggtcagaaag tggcgctgaa agaggcgatg ggcagtacac
    12481 aaagcattat ggttgggccg gacggtgagt tgtacggcgc atccgacccg cgctcggtgg
    12541 atgatttaac ggcggggtac taaggttagc ggccctcttc gtgggaagag ggctattttg
    12601 tcagggcaag ccgaaggtag ccttttttat ttcgtaatcc tgtagatatt cttccagcgg
    12661 ctttgccgtt cccagagaat gaatttcacc actgtcttta tcaataataa aaggtgcgtt
    12721 accagctaag cgcgcggcct catctccagt ttcgagaaat tctcgtgctt cgaaacagaa
    12781 ataccagccc tggctaaagc gtccatgtag agtaatgacg accgggagat ctgcatcatc
    12841 aaggtaatgg ttcgctttcg cgaatgcgtc gtgataagta atcataatta taataaatat
    12901 tattgttgag tgttatatta ttattcctgt gtgatacatt gagcaaagac gcgttcatct
    12961 tcgtcaaaga tattattaaa tgctggtttt gaaaaaagtt catcggataa gatatagtca
    13021 cgaaataagt atctgctaat attttatgat ttctttttca gaacgtcgaa cgggattttc
    13081 ttatttaaga tatcagaatc ttatgattcg ggtaaaattc taaataaatc aacatgtcat
    13141 taaaatcatc atagccatta gctatttcgg ctaaaataga gactacatgt cttcggtcca
    13201 tctcacttaa ggagtgtagt tccgttgtaa gtttttccat agcttgcact gctaaatttc
    13261 gaacaaggaa ttttctgctg gtaatctcta aaaagatggc atggtttaca atgatttttg
    13321 tttccttttg attattatga acaactgtcc atgatttcgt ttaagaatga agagaaatca
    13381 ctaaacgaac t
//
LOCUS       DAOFQD010000123        13321 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.123, whole genome shotgun sequence.
ACCESSION   DAOFQD010000123 DAOFQD010000000
VERSION     DAOFQD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.123"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..307
                     /locus_tag="QBF46_003764"
     CDS             <1..307
                     /locus_tag="QBF46_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675892.1"
                     /translation="SYKDLFNIAGGYNKVLQPETYEQVIYPAIQILCSPKVDFLKLNY
                     WFIDDFHDPVELEIEEVIEAEISQSLANPFTGELMYDYKSFVFPFFTLDESKSKDII"
     gene            307..972
                     /locus_tag="QBF46_003765"
     CDS             307..972
                     /locus_tag="QBF46_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675893.1"
                     /translation="MLKMSAAMSLADIDNDIFLQRINASITRVRFALQSYDNFKQFVR
                     IELDSAVKEIEENSNKLNFDLTEDQLTLILLANIKNKDMGIEAFHENNQRGHCDITIK
                     LKDYIWHGEAKKHTSSYSYLFKGYAQLTERYSTGTVDSASGGLIIYTRNRKCNEMMSN
                     WKAHLDKFAPRIHACKSITITPCQKNPLVFYSQHVHTVTQLNYEVIHYPVNLYHEPVD
                     PDL"
     gene            complement(1042..2076)
                     /locus_tag="QBF46_003766"
     CDS             complement(1042..2076)
                     /locus_tag="QBF46_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ6675894.1"
                     /translation="MGKSKKNRAAATKQIQLKSQTTAEAFSFGDPVPVLDRRELLDYV
                     ECVQMDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIAIEPALAKYTRRGLDLDTYWFVQYGMTTLPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPSNVGGFGDVEKASRVFVRNELIPLQKRLQELNDWLGEEVM
                     RFEDYNLGIE"
     gene            complement(2076..3842)
                     /locus_tag="QBF46_003767"
     CDS             complement(2076..3842)
                     /locus_tag="QBF46_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDQ6675895.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRSKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            3985..4818
                     /locus_tag="QBF46_003768"
     CDS             3985..4818
                     /locus_tag="QBF46_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDQ6675896.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            4835..5893
                     /locus_tag="QBF46_003769"
     CDS             4835..5893
                     /locus_tag="QBF46_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDQ6675897.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSDN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMVGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAETGA"
     gene            5897..6547
                     /locus_tag="QBF46_003770"
     CDS             5897..6547
                     /locus_tag="QBF46_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDQ6675898.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLAATGFDADLLLQTLELTDGMDM
                     PDQSRARLHKAIGAVLSERNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            6643..7107
                     /locus_tag="QBF46_003771"
     CDS             6643..7107
                     /locus_tag="QBF46_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDQ6675899.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGAKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            7107..7310
                     /locus_tag="QBF46_003772"
     CDS             7107..7310
                     /locus_tag="QBF46_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDQ6675900.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            7314..7529
                     /locus_tag="QBF46_003773"
     CDS             7314..7529
                     /locus_tag="QBF46_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675901.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            7510..8022
                     /locus_tag="QBF46_003774"
     CDS             7510..8022
                     /locus_tag="QBF46_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDQ6675902.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSEEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKIITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDATVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            8024..8401
                     /locus_tag="QBF46_003775"
     CDS             8024..8401
                     /locus_tag="QBF46_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675903.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQRIFPVLAASGVWVACLLT
                     WAVIPVALAALIKNGPLWQELRASLLKTITRKENVFTSWVMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            8398..8826
                     /locus_tag="QBF46_003776"
     CDS             8398..8826
                     /locus_tag="QBF46_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HDQ6675904.1"
                     /translation="MNRLLLVVLTLLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSTLLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRKRPALTGGMAYRQWLSASDAVSAGSGNAAH"
     gene            8922..9353
                     /locus_tag="QBF46_003777"
     CDS             8922..9353
                     /locus_tag="QBF46_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6675905.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNAVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAIAEPDAPEEMWTVKRG"
     gene            9346..9792
                     /locus_tag="QBF46_003778"
     CDS             9346..9792
                     /locus_tag="QBF46_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDQ6675906.1"
                     /translation="MAELQKVDDWLSALLANLEPASRSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSGYEPRRVTARSKKGRIKRQMFAKLRTTKYLKTAASTDSASVQFEGKVQRIA
                     RIHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
     gene            9861..10439
                     /locus_tag="QBF46_003779"
     CDS             9861..10439
                     /locus_tag="QBF46_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDQ6675907.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGDLETNWIN
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAIYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITMKTSEFVLEADHTRINSEVVINGGVT
                     QGGGVMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            10436..10795
                     /locus_tag="QBF46_003780"
     CDS             10436..10795
                     /locus_tag="QBF46_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDQ6675908.1"
                     /translation="MTLYSGMNNTSGKAITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVELTGRRNN
                     GVPVSLSVSTGAENGND"
     gene            10782..11690
                     /locus_tag="QBF46_003781"
     CDS             10782..11690
                     /locus_tag="QBF46_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDQ6675909.1"
                     /translation="MAMIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVTRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            11683..12288
                     /locus_tag="QBF46_003782"
     CDS             11683..12288
                     /locus_tag="QBF46_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDQ6675910.1"
                     /translation="MNSLLPRGSTSLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGEAPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            12285..>13321
                     /locus_tag="QBF46_003783"
     CDS             12285..>13321
                     /locus_tag="QBF46_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6675911.1"
                     /translation="MSTKFYTLLTDIGAAKLVSAAALGVPLKITHMAVGDGGGTLPTP
                     DAKQSALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRKYVDDKALELK
                     VYADDQMAKHLAAPDPHSQYAAKESPTFTGTPKAPTPATGNNTTQVATTAFVQAALTA
                     LINDAPATLDTLKEIAVAINNDPKFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAAMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTHLSGKDVASL"
BASE COUNT         3156 a         3424 c         3819 g         2922 t
ORIGIN      
        1 aagttataag gatttattta atatagctgg tggttataat aaggttttac agccagaaac
       61 gtatgagcag gttatctatc cggccataca aattctttgt agcccaaagg ttgatttttt
      121 aaaactcaat tattggttca ttgatgattt tcatgaccct gtagaacttg aaatagagga
      181 agttattgag gcggaaatca gtcaatcttt agcaaaccca tttacagggg agcttatgta
      241 tgactataag tcattcgtat ttcctttttt tacattagat gaatctaaat caaaggatat
      301 tatctgatgt taaaaatgtc tgcagcaatg agtttggctg atattgataa tgatattttt
      361 cttcagcgta ttaatgcttc tattactcgt gttagatttg cacttcaatc gtacgataat
      421 ttcaaacaat ttgtcaggat tgaacttgat tctgctgtaa aggaaataga agagaattca
      481 aataagttaa acttcgattt aacagaagat cagcttactt taattttatt ggccaatatc
      541 aaaaataaag atatgggcat tgaagcattt catgaaaata atcagcgcgg acattgtgat
      601 attacaataa aactaaagga ttatatttgg catggcgaag ccaaaaaaca tacatcttca
      661 tatagttatt tatttaaagg gtatgctcaa ctcactgaac gatatagtac agggaccgta
      721 gacagcgcaa gtggtggatt gattatctat actcgtaata gaaaatgtaa tgaaatgatg
      781 agcaactgga aggcgcattt agataaattc gcaccaagaa ttcatgcctg taaatctata
      841 acgattactc cttgtcaaaa aaatccattg gttttttata gccagcatgt tcacacagtt
      901 actcaactga attatgaagt tattcattac cctgtaaatc tatatcatga gcctgtagat
      961 ccggatcttt aagttttaat gctcatgtct tggtgatttt aaactaaaga ataaaagcct
     1021 gtttgtacag gcttttaaat atcattcaat acctaaatta taatcttcaa aacgcatgac
     1081 ttcttctcca agccagtcgt tcaattcctg aaggcgcttc tgcagaggta tcaactcgtt
     1141 gcgaacaaag acgcggctgg ctttctccac atccccaaaa cccccaacat tactcggcat
     1201 aatccccatc atttgcggcg gcacacgatg tgctgccatc atgtcatccc gactcacgtt
     1261 cttgatattc aaaaactcat ccttcgctgc gacctctgac aacgggataa tctgaagtcc
     1321 gtcctttttg ccgttaggcg agtacataaa cagattgcgg aagttgccag ggcctttggc
     1381 gcttttcatt gcgttgcgga ggttgttcac atcttcctgg ttctgcgcgg catcggtcat
     1441 atacatgatg aagcctgcat ggctaccgtt aatgtaatac ttgcggcgga acagcgtggc
     1501 ggactcgttg agcagggctg acggaatggc agaaagataa ccgggcaggc cgtagatctc
     1561 ctggttgatg tccggttcca tcagatgaaa aatgctgcct ttcgtgaact gatacggcag
     1621 ggttgtcata ccgtattgca caaaccagta ggtatccagg tctaatccgc gtcgggtgta
     1681 ttttgcaagc gcaggctcaa tagcgataac ttcaccgaat cggttcgtgc gtttctccag
     1741 gtaggcgtta ccaaatacca gatagtcctg cacaaaacgc gaaaaagcct gctggctgag
     1801 cagcggatga gggatatagg tactggtcag aatgttgcac tttactgcaa tcggggaact
     1861 gtgatgcacg gcggcgcgga aggtgcgcgc cagtccgtca aagctgacgg gcggctcata
     1921 ccaacggtcc atctgtacgc attccacata atccagcagt tctcggcggt ccagaacagg
     1981 aacgggatcg ccgaagctga atgcttcggc tgtagtttga cttttaagct ggatctgttt
     2041 cgtcgccgca gcgcggttct tcttactctt tcccatcaaa aaatctccac aatattgctg
     2101 gtattggcgg attcgccctg cagcggttcg ttaaacagtg cgtgcatcgt tgcccaggcc
     2161 agatcggcgt ggctggcttc ttcgctgcgg ctggcttcat aggtcgggcg gttgccactg
     2221 gcggtggtgg cgcgacggat tgccataaag gactgcgcta tgtcggtgtg tccggcgtca
     2281 aactccagac ggcggtggct gataatgtcg taggccttga gtaccagggc gtttttaacg
     2341 ttggggttgt agacaaactc ccggacggca ggaaaaaacg ctttcacgtt ctcgtaaacc
     2401 ccgtgaccaa cgccggttga gtcgataccg atataggtca cgttgtactg ttcggtcagt
     2461 tttttgatgg cgtcagcctg ggcgcggaag tccattccgc gccactggtg acgctcaaga
     2521 atgcggaact taccgcccgg cacggctggc ggcgccacca ccacgcatcc ggcgctgtcg
     2581 ccgttctgcg tacctttcgc cgggtcatat ccgatccaca cttcgcgcca gccaaacggg
     2641 cgcagggcca gtgcatgaaa gtcggtccag acttcccaac tgtccaccat gcacgcctgc
     2701 agctcgctga gtgggaacac agacgcgaga tcgtccacga actcacacat cagcaggttc
     2761 tggtattcgt ccgggctgta ctccatgcgc aactggtcga ggtcgaacag gttacagccg
     2821 ccgcgcaccg catcttccac agtgactatc tggcggtact gcccgtctgc gcacagcagg
     2881 ccgggggcca gattgctgtg ggacaggtcg atgtccacct tatcggcttt gctgcgccct
     2941 cggttgaaca gcgcaccgga ccagaacgga taagcactgt gtgtcaggct ggatggcgtg
     3001 gaaaaatagg tttgtcgcca ttttttgtga atagccatac cggaagccac tttgcgcagc
     3061 tcctggaatt tcggtatcca gaaatattca tccagataca ggttgccgtg gtaactctgg
     3121 gccgtgcggg cattggtgcc gaggaagtac agcgtggccc cgttaggaag caccatcgga
     3181 tcgcctttca gctccacctc cacttctttg gcgaagtcga tgatgtactg cttaaagacg
     3241 tgggcctgag ccttgctggc ggaaaggaaa atctggttac gcccggttag cagggcgtca
     3301 atcagggctt cacgggcaaa atagaaggtc gcgccgatct ggcgtgactt cagcaggttg
     3361 cggatgcggt tggtttttcc ggcttcccac cagtggcgct ggtagttgaa catggaggaa
     3421 tggaagattt cttccaactt ctcaatctgt tcatcggtga aaacgttctt ttccggctga
     3481 cggcgcgggc ctttgttgcg gttggcgacg ttagggttta agtcggcttc gttgccgcca
     3541 ttgttaaact tgccgatccg cgcatggcgc tccgactggc gcgccagcag gtcaatctct
     3601 ttgaaatctt tcccttcttt gtgctccttc ataatgagct ggcagtagcg tgcggcggtg
     3661 gtgagctgca tctgatcaag cggcccatag tcaccccact tgtcgcgttt tttccagctg
     3721 tgaacggttg caactttctc gcccagcatt tcagcaatgc gggctacgcg gtatccctga
     3781 aagtacagca gcatggcctg ccgacgggga tcgagatctg cgggtgtcag tgtggtgttc
     3841 atggcacaaa cctacagcct tgaatgaagg ctttccccgc ctgcggtttg tgtggttgtc
     3901 ggtacaaata ccgcgcattg tttcactgcc cccatcaccg caaccataag gctccagtaa
     3961 gttttttcta acggagcacg gctcatgaca gtgaaagcaa agcgttttcg catcggggtg
     4021 gaaggtgcca ccaccgacgg acgcgaaatc cagcgtgaat ggctggaaca gatggcagcc
     4081 agctataacc cggcggtgta taccgcgctg attaaccttg agcacatcaa gtcttatctg
     4141 ccggacagca cctttaaccg ctacggcaag gtgacggcgc tgtttgctga agaaatcacg
     4201 gaaggtccgc tggcaggcaa gatggcgctg tatgctgacg ttgagccaac ggagtccctg
     4261 gtggaactgg tgaaaaaagg ccagaaatta ttcacctcta tggaagtcag cccgaagttc
     4321 gctgatacgg gcaaagccta cctggtcggc ctggctgcca ctgatgaccc tgccagtctg
     4381 ggcactgaaa tgctgacatt cagcgccagt gcagcccata acccgctggc aaaccgcaag
     4441 cagaatcccg ccaatctctt taccgccgca gaggaaacgg tgatcgaact ggaagaagtc
     4501 caggacgaca aaccgtccct gtttgcccgc gtcacggcgc tgtttaccaa aaaagagcag
     4561 tccgatgacg cccggttctc tgatgtgcat aaggccgtgg agctggtcgc cactgagcag
     4621 cagaacctga gcgcacgcac cgaaaaatcc ctgtctgagc aggaagaacg cctgtctgag
     4681 ctggagactg ccctgcaggc acagcaaacc gcctttaacg aactggtgga caagctgagt
     4741 catgaagaca gccgccagga ctaccgccag cgtgcaacag gcggtaacgc ccccgctgac
     4801 actctgacca attgctgatg gagcacaaaa cctgatgaag aagaataccc gctttgcttt
     4861 taacgcttac ctgcagcagc tggcgcgtct gaacggtgtg gcagttgaag aactgtccag
     4921 caagttcacc gtagagccgt ctgtacagca gacgctggaa gaccagatcc agcagtccgc
     4981 cgctttcctg acgctgatta acgtcacgcc agtgactgag cagtccggtc agctgctggg
     5041 gctgggagtt ggcagcacca ttgccggaac cactgacacc accgcgaaag agcgtgaacc
     5101 tgtcgatccg acgctgatgg tcgatgtgga atataaatgc gagcagacca actttgacac
     5161 ggtgctgacc tacgcgaagc tggacctgtg ggcgaagttt caggatttcc aggtgcgcat
     5221 ccgtgacgcc atcgtgaaac gtcaggcact ggaccgcatc atgatcggct ttaacggcgt
     5281 gaagcgtgcg aaaacctcca accgtagcga taacccgctg ctgcaggatg tgaacaaagg
     5341 ctggctgcag aaaatccgtg aagatgcacc ggatcacgtc atgggcagca ccaccacggg
     5401 cggtgaaacc acaccgggtg cggtgaaagt cgggaaaggt ggcgaatatg ccaacctgga
     5461 cgccgtggtg atggatgccg tcaatgagct tatcgatgtg gtctaccagg acgatgacga
     5521 tctggtggtg atttgcggtc gtgaactgct gtctgacaag tatttcccgc tggtcaacaa
     5581 agagcaggaa aacagtgaaa aactggctgc cgatatgatc atcagtcaga aacgcatggt
     5641 tggcctgcag gccgtgcgtg cgccgttctt cccgccgaat gcactgctga tcacccgtct
     5701 ggataacctg tccatctact ggcaggaaga cacccgccgc cgctcagtta tcgacaaccc
     5761 gaaacgtgac cggattgaaa actttgaatc cgttaacgaa gcctatgtgg ttgaggacta
     5821 ccgctgcgcc gcactggtgg aaaacatcca gattggcgac ttcagcgccg cagcagcaga
     5881 aaccggagcg taattcatga gcctgagtcc cgcacggcag catcgcctgc gcgttcaggc
     5941 tgaacaggcc gcccgcgagg gcggcagcgt tcgccacgcg tcgggctatg acctgatgct
     6001 gctgcaactg gcggaagacc gccgccgtct caagggcgtt cagtccacgg tcaaaaaagc
     6061 ggaaatcaag gtggagctgc tgccgaagta cgccgcttgg gcggagggtg tcctggctgc
     6121 cggaggcgct caacaggatg acgtgctgat gtacgtgatg ctgtggcgca ttgatgccgg
     6181 agattatgcc ggggcgctgg agatcgggcg tcacgccctg cgtcatggct gggtgatgcc
     6241 gttaggtaac cgcaacgtgc agaccgtgct ggcagaggaa atggcagatg cagcccagag
     6301 cgcaatgctt gccgccaccg gctttgatgc cgatctgttg ctgcagaccc tggagctgac
     6361 agacggtatg gatatgccgg accagtcacg ggcgcgtctg cataaagcga ttggcgctgt
     6421 cctgagtgaa agaaatccgg cgtctgccct taatcatctc aaccatgcgt tacagctcga
     6481 tccccgctgt ggcgtgaaaa aagacaaaca gcagctggag cgcagactgc gcaatgacag
     6541 ccgctgacag aacgtgcccc cgcgcacggg cggcacgggg tggcgaaagg cactgccaca
     6601 tcaaaacccc gtccaccgcc ctctatttca ggagaaagca gcatgaagtt tgttgcgcca
     6661 gaacaggcac cggaacaggc ggaaatcatc aggaatacgc cgttctggcc tgatgtggac
     6721 ctgtcggagt ttcgcagtgt gatgcgcact gacggcacgg tgacgcagcc gcgtttaaaa
     6781 caggttgctc tgtcggcaat ttcggaggtc aacgcagagc tgtatgagtt tcgcagacgc
     6841 cagcagatgc tggggtatgc ctcgctggca gaagtcccgg cggaacaact ggacggcaaa
     6901 agcgagcgca ttcagcacta tttcaacgcg gtttattgct gggcacgcgc catgctcaac
     6961 gaacgttacc aggactatga cgccacggca tccggtgcga agcgaggcga ggaactggcg
     7021 gaagcaagcg gtgatttatg gcgtgacgcc cgctgggcca tcagccgggt gcaggatgcg
     7081 ccgcactgca cagtggagct tatctgatga aagtgcgtgc gcatcagtat gacacggtgg
     7141 acgcactttg ctggcgtcat tacgggcgca cgcagggtgt cacggagcag gtactgaagg
     7201 caaatccggg gcttgccgaa tacggcccct ttttacctca cgggctgcag gtggagctgc
     7261 cggacattcc gacaaccacc accgtgcaga ccgtccagct atgggactga attatgacgc
     7321 ttgagcgaat cagcgccttt atcacgtatt gcatcgccgt cgtgctggcc tggctgggcg
     7381 atttgtccat caaggatgcc tcaacgctgg gcggcctgat gatcggtgtg ctgatgctgg
     7441 ctatcaactg gtactacaaa cacaaagcct accagcttct gcgcgacggg cagatctcgc
     7501 gggaggacta tgaatccatc aatcgttaaa cgctgccttg tcggggccgt gctggctatt
     7561 gctgccacgc tgccggggtt tcagcagctt cacacctccg aggagggact gaaactgatc
     7621 gccgattatg aaggctgtcg tctgcagccg tatcagtgca gcgcgggtgt ctggaccgac
     7681 ggcattggta atacgtcggg cgtcattccc ggcaaaatta ttacggaacg acaggcagct
     7741 gaagggctga tctccaacgt gctacgagtg gagcgggcgc tggaaaggtg tgtgaagcaa
     7801 cagccgccgc agaaggtgta tgacgctacg gtgtcgtttg ccttcaacgt ggggacgggc
     7861 aatgcctgta gttccacgct ggtgaaattg ctcaatcagc ggcgctgggc ggatgcatgc
     7921 cgacagttgc cgcgctgggt ttatgtgaaa ggtgttttta atcaggggct ggataaccgc
     7981 cgtgcgcggg agatggcctg gtgtttacag ggagcaaact gaaatgaaaa agaaattaat
     8041 cagcggactg tttctgatgt tatggatggc gctgttaatc gcagcaatgg tgtatccgca
     8101 gaggattttt ccggtactgg cagcgtccgg cgtttgggta gcctgtttgc tgacatgggc
     8161 ggtaattccg gtagcactgg ctgcgttaat taagaatggc ccgctctggc aggagttaag
     8221 ggcatctttg ctgaagacaa ttacccgaaa agaaaacgta tttaccagtt gggtgatgcg
     8281 attgctgatt gttgtaagtc tcgcctggac ggggtgggct attaccctgg tcttttatct
     8341 actgaccgtt attgccttct ggatcacccg taatcagatg gcgcaacagg tagcagcatg
     8401 aaccggttgc tgctggttgt gctgacgtta ttactggcgg cgctgggctg gcagacgtgg
     8461 cggctggctg atgccagcca gaccatcagc acgcaggcag acgagctgca gagcaaaagc
     8521 caggcactgg caaagagcaa tagccagctt atcagcctgt ccattctgac tgaaaccaat
     8581 aaccgggagc aggcgcggct ctatgccgaa gcagaacaga ccagcacgct gctgagacaa
     8641 cgacaacacc ggatcgagga actgaaacgt gagaacgagg atttacgccg ctgggctgat
     8701 actcctttgc ctgctgacat tatccggctg cggaaacgtc cggcactcac cggaggtatg
     8761 gcttaccgtc agtggttgtc cgcgagtgac gccgtgtcgg ctggatcagg caacgccgcg
     8821 cactaacggt gatctgaacg cgttgctgga tgaaacggag gccgcctggg cggtctgtgc
     8881 agacaaagtg gacatgatta ttgcgtgtca ggagcgaaac agtgaacaaa ccacaatccc
     8941 tgcgccacgc cctcaataaa gcggtgcctt atgtccgcaa taacccggac aaactgcatc
     9001 tgtttgtgga taacggttcg ctggttgcca cgggggccag ctccatgtcg tgggagtacc
     9061 gttacacact gaacgcggtg attgaggatt tcagcggcga ccagaatctg ctgatggccc
     9121 cggttttgct gtggctgagg gataaccagc ccgatgccat caataacccg gcgttacggg
     9181 aaaagctatt cacctttgag gtggatattc tgcgcaacga tgtctgtgat atcagcctga
     9241 acctgcaact gacggaacgt gtgctggtca gcactgacgg cagtgtgtcg agcgttgaag
     9301 ctatagcgga acctgatgca ccagaagaaa tgtggacggt gaaacgtggc tgaactgcag
     9361 aaggtggacg actggctgag tgccttgctg gcgaatctgg aaccagcctc gagaagccgc
     9421 atgatgcgcc agctggcgca ggaacttcgc cggacacagc agcagaatat caggatgcag
     9481 cgcaaccctg acggcagcgg ctatgaaccg cgacgggtaa cagcacgcag taaaaaaggc
     9541 cgtatcaaac gtcagatgtt tgcaaagctg cgcaccacaa aatacctgaa aactgccgcc
     9601 agcaccgact ctgccagcgt gcagtttgaa ggcaaggtgc agcgcattgc ccgcattcac
     9661 cattacggcc tgcgagatcg cgtcagtcgt aagggaccgg aggtgcgtta cgcagagcgt
     9721 cgccttctgg gtgtaaatga tgatgttgag gcaatgaccc gcgacatgat tctgcaatgg
     9781 ctggcggggt gatctttgta tcagcactga tacaagttgc agcactgccg cctttcttcc
     9841 cctgatggca acctttccct atgaacgcac aattaaccga aatcatgcgc cttatcacca
     9901 acctgatccg cacaggggta gtcaccgaag tggacaggga aaactggctt tgccgggtga
     9961 aaacgggcga ccttgaaacc aactggatta actggctgac gctgcgcgcg ggtaatgccc
    10021 ggacatggtg gcgaccatcg gaaggtgagc aggtggtgct actgagtctg ggcggcaatc
    10081 tggaaacagc ctttgcgctg cccgccatct attcgaatca gttcgcgcca ccgtcgacgt
    10141 cggcagacgc ctgcgtgaca gaacatcctg acggtggctg gtttgaatac gaacctgcca
    10201 ccgggcgttg gtatgtaagg ggcatcaaat caatggtcat tgaggccgct gacaacatca
    10261 ccatgaaaac cagtgagttt gtactggagg ctgaccacac gcgcattaac agcgaagtgg
    10321 tgatcaatgg tggcgttacc cagggcggcg gagtgatgag ttctaacggg attgtggttg
    10381 atgcacatca gcatactggc gtcctgaaag gcggcgacac aaccggaggc ccggtatgac
    10441 gctttatagc gggatgaaca ataccagcgg taaagccatt actgatattg accatctgcg
    10501 ccagtcggtg cgggacattt tgctgacgcc gcagggtagc cgcattgccc gtcgggaata
    10561 tggttccctg ctgtcggcac tgatagacca gccacaaaat ccggcattac gcctgcaggt
    10621 catgtcggca gtgtatgtgg cgctgagtcg ctgggagcca cggctgacgc tggattccat
    10681 caccatcaac agcaactttg acggttctat ggtggtggag ctgaccgggc ggcggaataa
    10741 cggtgtgcct gtgtcccttt ccgtatcaac aggagcagag aatggcaatg attgaccttt
    10801 cgcagttgcc tgcaccgcag attgtggatg tgccggactt tgagacgctg cttgccgaac
    10861 gcaaggcaga atttgtggcg cttcatccga aagatgagca ggaagccgtg atccgcacgc
    10921 tggaactgga atctgaaccc gtcactaaat tgttgcagga gaacgcttac cgtgagttgc
    10981 ttctgcgcca gcgcattaac gaagccgcac aggcggtgat ggtggcttac gcgatgggcg
    11041 gcgatcttga ccagctcgct gccaactaca acgtgacacg cctgacggtg acgcctgctg
    11101 ataatgatgc tgtgccgccc gttgcagctg tgatggaaag cgatgaagcg ttacgcctgc
    11161 gtgtgcctgc agcctttgaa gggctttctg ttgcggggcc aactgcagct tatgaatttc
    11221 atgcccgaag cgccgacggt agggtggcgg atgccagtgc aaccagcccg gcacctgcag
    11281 aggtggtgct gactgtcctt agccgcgaag gcgatggaac tgcagaaaaa gacctgctgg
    11341 acgtggtgga aaaagctctg aacagtgaga acgtccgccc ggtggctgac cgtctgacgg
    11401 ttcgcagcgc agaaatcatc ccgtatcgcg tggaagccac catttttctc tatccgggac
    11461 cggaagcaga gccggtaatg gcagcggcaa aagccagcct gcagaagtac atcgccagtc
    11521 agacgcgtct tggtcgggat attcgccgta gcgccatctt tgccgccctg catgttgagg
    11581 gtgtgcagcg tgtggagctg gcttctcctc tggcggatgt ggtcctgaac aaaacacagg
    11641 cggcatcatg tacgcagtgg agcgtaacca acggaggaac ggatgaatag tctgctgcca
    11701 cggggttcaa cttcactgga gcgccgactg gcgcaaacct gtagcgggat ttctgatctg
    11761 caggtgccgc tgcgtgactt gtggaatccg gctacctgtc cggtcagctt cctgccttat
    11821 ctcgcctggg cgttctctgt ggatcgctgg gacgagggct ggacagaaag cgtcaaacgc
    11881 caggtagtga aggatgcttt ttatattcat cagcataaag gaaccaccag tgccgtgcgg
    11941 cgggtggtgg aaccgttcgg attcctgatc cgcattattg agtggtggca aaccggagag
    12001 gctccgggca cgtttcgcct ggatatcggc gtgcaggacc agggcatcac tgaagatacc
    12061 tatctggaac ttgagcgact gataagcgat gccaaaccat gtagccgcca catgatcggc
    12121 atgtccatca atctgcagac cagcggcccg cattgggtgg gagccgccag ctatcttggc
    12181 gaagaaatca cgatctatcc gtatatcaac gaaacaatta tttctggcgg caccgcgcat
    12241 gaaggcgggg cggtccatgt tattgacaca atgagagtga atccatgagc acaaaatttt
    12301 ataccctgct gacggatatt ggcgcggcga aacttgtcag cgccgccgcg ctcggtgtgc
    12361 cgctaaaaat tacccatatg gcggtgggcg atggtggcgg aacattgccg acgccggacg
    12421 caaagcagag tgcactggta aatgagaaac gtcgggctgc gctgaatatg ctctatatcg
    12481 acccacagaa cagcagccag attattgctg aacaggtgat ccctgaaaac gagggcggtt
    12541 ggtggatacg tgaagtgggc ctgtttgatg agtcaggggc attgattgcc gtgggcaact
    12601 gcccggaaag ctataagccg caactggctg aaggcagcgg gcgtacccag accgtgcgca
    12661 tggtgctgat taccagcagc acggacaata tcaccctgaa aatcgaccct gctgtagtgc
    12721 tggcaacccg caagtatgtg gatgacaagg cactggagct gaaggtgtac gcggatgatc
    12781 agatggcaaa acatcttgcc gcaccggacc cgcattcaca gtatgcagcc aaagaaagtc
    12841 ctacgtttac cgggacaccc aaagcgccaa cgccagcgac ggggaataac accacgcagg
    12901 ttgcgaccac cgcatttgtt caggcggcac tgacggctct tattaatgat gcgccagcca
    12961 cgctggacac gctgaaagaa atagccgtag ccattaacaa tgatccgaaa ttcagtacca
    13021 ccattaacaa tgcgctggca ctgaaagcgc cgctgtcgag tccggcactc accggaacgc
    13081 caaccgcacc gactgcggca cagtcggtca acaatacaca gattgccacc acggcatttg
    13141 tgaaatcggc gattgcggca atggtgggtt ctgcacctgc ggcactggat acactgaacg
    13201 aactggcggc ggcgctgggg aatgacccga actttgccac gacaatgctt aatgcactgg
    13261 caggtaaaca accgctggac aatacgctga ctcatttgag tggaaaggat gttgctagtc
    13321 t
//
LOCUS       DAOFQD010000124        13212 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.124, whole genome shotgun sequence.
ACCESSION   DAOFQD010000124 DAOFQD010000000
VERSION     DAOFQD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.124"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            429..1502
                     /locus_tag="QBF46_003784"
     CDS             429..1502
                     /locus_tag="QBF46_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675912.1"
                     /translation="MRKSYTYGIPFGLHRETGRFLDITEVSRGSACNCICPGCRTDLI
                     ARQGEVKLWHYSHSTDLLGDCDGLMEAIRGKIIEIIQEKQLLGFPHLLAGYDGGTVPL
                     DEVSGSGSMFGATADLFVKVNDLCVAVFLDIDRSVAGKLTFDHLHASEMVAALRIDLP
                     DIEYEISQVQLGRRDGTYSECIEKIIIDATESREWLYHPMMQELGTEPLKVYEGREPA
                     EAGPLLQRLSDCPLKLPDNLPQSMNVLIRMQQATIVCLCRLTVRASDIAQTDEFPLFM
                     RYFRLHCLDTSEHVNYEKLLNWQDMITKSKEGQLLTVEERDYLQEIVRIGLYNHRLKS
                     VCLTACEDSRVGESEGPNNYSLL"
     gene            complement(1759..2235)
                     /locus_tag="QBF46_003785"
     CDS             complement(1759..2235)
                     /locus_tag="QBF46_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675913.1"
                     /translation="MEKTQSPAERFYESIYYSDESLEEDYLAELRNFSSDHWDTALRA
                     ARLSAAVKRFKTSEMLRFILEFVVPENAQEDAPDLTPLAAKRLCNSLFGRSGSQSILV
                     YVFGQAGRVHRSATCSPKTIEAIAALYRSDAERYWNSTLATIERVKHTYRAKIRNS"
     gene            2915..3307
                     /locus_tag="QBF46_003786"
     CDS             2915..3307
                     /locus_tag="QBF46_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675914.1"
                     /translation="MDDKEQFTSLVAKHASRFTEEQLAGYDACSQYGECVSPSYEVFR
                     GYRTRHTLDEFLELAISLNAIHPDEYLTDMLLKPHEVIGALADEGDQLNNATPVYFFP
                     DTGVYAAAVSETRVLDAWLCWPCYPANW"
     gene            3720..3923
                     /locus_tag="QBF46_003787"
     CDS             3720..3923
                     /locus_tag="QBF46_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675915.1"
                     /translation="MFSTEKKSEHLGQIIVIDPALLGQGIEVGPVVVFDLGHASKGVA
                     DLMRDAEKLDGLSPTTIANRLSR"
     gene            4329..5135
                     /locus_tag="QBF46_003788"
     CDS             4329..5135
                     /locus_tag="QBF46_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ6675916.1"
                     /translation="MREPRPRHTLQVVCVPSLNIQDLGLTSFHSWLLSKGYDTTNARN
                     NRTCWAREGGWHLKRCRHLATGEDHFWFIAFNGTGGDIFPVKTQQDYRAAYRKLEAYG
                     YAPAVIEQLNTGVAYNLAYSRTPLKRPESATSELKRKPDVNIQGEPCERFVTERSSAA
                     QNKFKTLLIENFAGRCAVTGWVNGGVLVAAHIEHGTRYNPSNGILLTPTMHALFDADL
                     MGIDPSTLTVHFKPGIEAGELFEGKTLNPLVYDLDTDRLAARWADFIGAE"
     gene            5253..5660
                     /locus_tag="QBF46_003789"
     CDS             5253..5660
                     /locus_tag="QBF46_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675917.1"
                     /translation="MQNCLSGYLKATIVILSADLTGCDLANGAAESGVYQKYTNGPFK
                     EPTIVMSPGWRMDDHGREALIFGSSHCPDVNGNNTSEGGCVLIEEHSATVAVTVVDAT
                     NQSRRQETWTIERKKDRVIVKRPDNSFVMPWGK"
     gene            5707..6417
                     /locus_tag="QBF46_003790"
     CDS             5707..6417
                     /locus_tag="QBF46_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ6675918.1"
                     /translation="MCVERPDGAFDIYTGRFIQFHQIDNSRRKIVEISDPDLVGMTYS
                     IPMVFNGGKFSGQGVTCLSLPAVTLIKVTFPSRTYQEGQEQITMSSPDQALFSEDVRK
                     NCAGTCVLTGVRGRQRTEAAHIKPRHAGGEPDVTNGILLRSDIHTLFDNWHFSIDPDS
                     MKARFSPDVLFVDKDLLQLEGKQIDFSRLQMPINIEHLRHHWNKFFNRHVNPFIKNAL
                     NKKINNYEKRPVNSNIHY"
     gene            6487..6671
                     /locus_tag="QBF46_003791"
                     /pseudo
     CDS             6487..6671
                     /locus_tag="QBF46_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050163.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7185..7589)
                     /locus_tag="QBF46_003792"
     CDS             complement(7185..7589)
                     /locus_tag="QBF46_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDQ6675919.1"
                     /translation="MSEALKSLNNIRTLRAQGRELPLEILEELLEKLSVVVEERRQEE
                     SSKEAELKARLEKIESLRQLMLEDGIDPEELLSSFSAKSGAPKKVREPRPAKYKYTDV
                     NGETKTWTGQGRTPKALAEQLEAGKTLDDFLI"
     gene            8210..8563
                     /gene="arsR"
                     /locus_tag="QBF46_003793"
     CDS             8210..8563
                     /gene="arsR"
                     /locus_tag="QBF46_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDQ6675920.1"
                     /translation="MPEIASLQLFKILSDETRLGIVLLLREMGELCVCDLCTALEQSQ
                     PKTSRHLAMLRESGLLLDRKQGKWVHYRLSPHIPSWAALVIEQAWLSQQDDVQAIARK
                     LASANCSGSGKAVCI"
     gene            8611..8973
                     /gene="arsD"
                     /locus_tag="QBF46_003794"
     CDS             8611..8973
                     /gene="arsD"
                     /locus_tag="QBF46_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HDQ6675921.1"
                     /translation="MKMLTVFDPAMCCSTGVCGSDVDQVLVNFSADVQWLKGRGVQIE
                     RYNLAQQPMSFVENEKAKAFLEASGAEGLPLLLLDGETVMAGRYPKRAELARWFGIPL
                     EKVGLASTSCCGGKTSCC"
     gene            8991..10742
                     /gene="arsA"
                     /locus_tag="QBF46_003795"
     CDS             8991..10742
                     /gene="arsA"
                     /locus_tag="QBF46_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010999951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter ATPase subunit ArsA"
                     /protein_id="HDQ6675922.1"
                     /translation="MKFLENIPSYLFFTGKGGVGKTSISCATAIRLAELGKRVLLVST
                     DPASNVGQVFDQTIGNTIQPVTAVSGLSALEIDPQDAAQQYRARIVDPIKGLLPDDVV
                     NSISEQLSGACTTEIAAFDEFTGLLTDASLLTRFDHIIFDTAPTGHTIRLLQLPGAWS
                     SFIESNPDGASCLGPMAGLEKQREQYAHAVEALSDPERTRLVLVARLQKSTLQEVART
                     HDELSAIGLKNQYLVINGVLPASEAERDALAAAIWQREQEALANLPAGLSDLPTDNLY
                     LQPLNMVGVSALKGLLNEHAEITSLPEQSPQNKPENMSLSVLVDDIARSEHGLIMLMG
                     KGGVGKTTMAAAIAVSLADKGFDVHLTTSDPAAHLSTTLNGSLKNLQVSRINPHDETE
                     RYRQHVLETKGRDLDEAGKRLLEEDLRSPCTEEIAVFQAFSRVIREAGKRFVVMDTAP
                     TGHTLLLLDATGAYHREIARKMGDKGHFTTPMMQLQDQERTKVLLVTLPETTPVLEAA
                     NLQSDLERAGIHPWGWIINNSLSIAQTQSPLLCQRALQERPQIEVVKNQHASRIALVP
                     VMAAEPTGIEKLRELVV"
     gene            10791..12080
                     /gene="arsB"
                     /locus_tag="QBF46_003796"
     CDS             10791..12080
                     /gene="arsB"
                     /locus_tag="QBF46_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDQ6675923.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALISGVVHFG
                     DIPVVWNIVWNATATFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTYIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFTEYASVMVPVDIAAIIATLVMLHLFFRKDIPPTYDLALLKAPAKAI
                     KDLATFRTGWIVLILLLVGFFVLEPLGIPVSAIAAVGAVILFAVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADKGLWAATFGTGFLTAFLSSI
                     MNNMPTVLVGALSIDSSTATGVIKEAMIFANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTITWGYYFRTGIVMTLPVLFVTLAALALRLSFTL"
     gene            12093..12518
                     /gene="arsC"
                     /locus_tag="QBF46_003797"
     CDS             12093..12518
                     /gene="arsC"
                     /locus_tag="QBF46_003797"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDQ6675924.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGIEPTVILYLETPPSRDEL
                     LKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDQLIDFMLQHPILINRPIVVTPLG
                     TKLCRPSEVVLDILPDAQKAAFTKEDGEKVVDDSGKRLK"
     gene            complement(12549..12953)
                     /locus_tag="QBF46_003798"
     CDS             complement(12549..12953)
                     /locus_tag="QBF46_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HDQ6675925.1"
                     /translation="MSDVKAFNNCMSVFWRQHEAEFSRFLTSKTGDSEKAADPEKTKV
                     IIVTLAETTPVLEATNLQQDLRRAGIEPWAWVVNNSLAAAQPSSPFLKIRANRELPLI
                     SDVEEQYAKRIALTALQSEEPVGIDLLEDMAK"
     gene            complement(12962..>13212)
                     /locus_tag="QBF46_003799"
     CDS             complement(12962..>13212)
                     /locus_tag="QBF46_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6675926.1"
                     /translation="TILSAVAQAERRRILERTNEGRQEARLKGIRFGRKRIIDRNSVL
                     ALHQQGTGATDIARRLSIARSTVYKILEDESRVNLSKI"
BASE COUNT         3331 a         3055 c         3344 g         3482 t
ORIGIN      
        1 cataaaacac tatcagtaag ttggagtcat tacccagtaa tgcgacaaat gcaatgaaag
       61 aagtagcgaa agtccttttt tcagcccgaa gtgacccgga gctcatgaaa tttataatgg
      121 attatattaa taaccacaaa ctgcattgaa ggtaattgcg gtgtataaaa caaagccatt
      181 ttcggccaaa aaatgagtca gctgaagaaa cgctcgttaa ttccgaatcc gtccagatta
      241 atatggcaca ctgctggtgg aggttttggc aatcggaaaa gtaacttttc acccgtgtct
      301 aatcatcatc gttttatcaa tccgctcagt tacggtatta aaatctcacg aaaaatagtc
      361 ttcttatcga agtgtcatgc tgtcttcgcc cgatatagtg aaggtatgta ttcagcgggg
      421 gcactgacat gcgaaagagc tatacctatg ggattccttt cggactccat cgcgaaaccg
      481 gacggtttct ggacatcacg gaagtcagca gaggaagcgc atgcaactgc atctgtcctg
      541 ggtgtcgtac ggatttgata gccagacagg gagaggtcaa actttggcac tatagccaca
      601 gtaccgatct cctcggtgat tgtgatggcc tgatggaggc gattcgggga aaaattattg
      661 aaattattca ggaaaaacaa cttctaggat tcccgcatct tctggccggt tacgatggag
      721 gcacggtccc acttgatgaa gtcagcggca gcggaagcat gttcggcgca acggcagatc
      781 tgtttgtgaa ggtgaacgat ctctgcgtag ctgtgtttct ggacattgac cgcagtgtgg
      841 ccgggaaact gacgttcgac catctccatg cctcggagat ggtagctgca ttgcgcattg
      901 atctgccgga tatagaatac gaaatcagtc aggttcagct tggtagacga gatggtacct
      961 acagtgaatg tatcgagaaa atcatcattg atgcgacgga gtcacgcgaa tggttgtatc
     1021 accccatgat gcaggaactg gggaccgagc ccctgaaggt atatgagggc cgcgagccag
     1081 cggaagcagg accacttcta caacgccttt ccgactgccc cctgaagctg ccggacaact
     1141 taccccagag catgaatgtg ctgataagaa tgcagcaggc caccattgtg tgtttgtgtc
     1201 ggctcaccgt cagagcttca gatatcgccc agaccgacga attcccgctg tttatgcgtt
     1261 acttccggct gcattgcctc gacaccagtg agcatgtgaa ttacgagaaa ttgcttaact
     1321 ggcaggacat gattacaaaa tcaaaggaag ggcagttact aacggtggag gaaagggatt
     1381 atctccagga aatcgtccgt attggtctct ataaccaccg gcttaaaagt gtctgcctga
     1441 cggcctgcga agacagtagg gtgggagaat ctgaaggacc aaacaattac tcattgctct
     1501 gaagagggat gggaaattat ggggggacat ggaagcgtga agagaataaa cctgttcgcc
     1561 ttaaacagaa acaggctgtt catttcttac atgtgaacag agtacctatc atgatcagac
     1621 cggtaatata aattggcaca gccatcaaaa ccaatcccag tggttcttct ttggtaaaag
     1681 catcctgaat aagtccgtct tcgcttaaag ctaatggacg catacattag cattaaggcg
     1741 taaggtctaa taactggatc aggagtttcg gatttttgcg cggtaggtgt gtttgaccct
     1801 ttcaattgtc gcaagagttg aattccagta gcgttcagcg tcagaacgat aaagagcagc
     1861 gatagcctct atggttttgg gggaacaggt tgcactgcga tgcacacggc ctgcctggcc
     1921 aaagacatag acaagaatgg actggctacc agaccggccg aacagggagt tgcaaagccg
     1981 cttcgctgcg agcggtgtca gatcgggggc gtcttcctga gcgttctcgg gaacaacgaa
     2041 ttccagaatg aagcgaagca tctccgatgt cttgaaacgc tttaccgccg cagacaaacg
     2101 ggcggcacgc agtgcagtgt cccagtggtc gctgctgaaa ttgcggagct ccgcgaggta
     2161 atcctcttcc aggctttcat cagaataata aatgctttcg taaaagcgct ctgcgggtga
     2221 ttgcgtcttt tccattggag aaaccatttt gcctaatgta tgcatacgtt agcatgtagt
     2281 gaaattaaaa caaggtaaag acaaagaacc gcattcaggc gtttcccgga gtaagcaggt
     2341 gggctgacaa ttcggctgcg gtaatatcaa gagctctgtt aattgagcgt taaatgcctc
     2401 ccgctccagt ccggattgga tgagaaagat cagggccttt ttcagaacgg gcggaatact
     2461 cgcaatgtcg aggagaatgt tgccctcaat aacatgatcg caattttcaa aaaggtgggc
     2521 gtaactcatt acgtgtattc catttatcat tttcaaatcg tgataaaaat tagattacca
     2581 gaacgtcaga agcaaaaaaa aagcattaag cagaaatgta tggtatccat ccttcctcca
     2641 gctacccttg cgccctgata attttatcct gggggagggg gcattgatca ttacataccc
     2701 gatcagaaaa gccgggaacc agaatgtcac ccggatatcg aagatgcaac gattcaatat
     2761 tagtgtattt agcgtctcct ggcagcaaag tcggtcggcc ggaactggta aactgtattc
     2821 cacctcgccc gggctggcag gcgactgacg tcttgtcggt tcatagccgg tggtcgcgtc
     2881 tgacaaaaca ggcccctaca gtgtaaggat tgtgatggac gataaagagc agtttacgag
     2941 tctcgtggca aagcatgcct ccagattcac cgaagagcag ctggccgggt atgatgcctg
     3001 ttcccagtat ggagaatgcg tctcgccttc atacgaagta ttccgggggt atcgtacccg
     3061 ccacaccctg gatgaatttc tggagctggc catatcgctg aatgccatcc acccggatga
     3121 atatttaacg gatatgctgc tgaagcctca tgaggtgatc ggtgctctgg ccgatgaagg
     3181 cgaccagttg aacaacgcca ccccggttta tttcttcccg gataccggcg tctatgcagc
     3241 ggccgtcagt gaaacccgtg tgctcgatgc ctggctttgt tggccatgct acccggcgaa
     3301 ctggtaatgc gtccgttgga attgcaccgg caggatacga atttaaccga ccccgtggcg
     3361 ggagggttat cacaattgca tgtcctcatc cctaaagtcc cctgccagac ccatacgtgc
     3421 tgtcaggctt cgcctgattt gagatcagtg caaatacttc agccgataac tttttgtttt
     3481 ctttttattt tctccctgtt tcctcaaaat tcgcgtgcgg cctggtcagg gcacgattgt
     3541 cacatagggt gtattgaagc gggcaggatg gtcgggtggg cacggcctgg gtttgagtcg
     3601 gacactatgt gggatgagga catgcaaaat attcgcttta tctgtgaaaa ctgttgtcta
     3661 cgggcagtca ccagttcaag ttacaccatc cacgttagct tttaataaga gtggttacga
     3721 tgttcagtac tgagaaaaaa tcagagcatt taggtcagat catagtgatt gatcccgcgc
     3781 ttcttgggca gggtatagag gttggcccgg tcgtagtgtt tgatttgggg cacgccagca
     3841 aaggggttgc ggacttgatg cgcgatgctg aaaagcttga tggtttatcc ccaacgacaa
     3901 tagcaaacag actttcacga tgaccgaaca aggtcgattc gggcaaggcg gcggaggggc
     3961 acatctccca atgataatga ggttatgcaa cctgttagtc accggcttgc ctgatgttat
     4021 gattgacatc tcttttgacc ggtctgtagt tgagcattaa ttcgtgagga atacggtatc
     4081 agcggataaa agtagcatgt ttgctatcgc ctccagctgt gaaaaccctc ggtgggtgca
     4141 agaagttgag agccgtgggg ggatgacccg taaggaatgg gctgactttg tggatcgaaa
     4201 ttttaaccct cctaaataga gtaaattccg attggcattg ttgcaggaat tatggtgccg
     4261 cagcttattg ggtgtttgtc aattgccggg aagtattcgc tggcatatca cttaaacggg
     4321 agtagtaagt gagagaacct cgtccacgcc atacattaca ggtagtctgt gtcccgtctc
     4381 tgaatataca ggatctgggg ctaaccagtt ttcatagctg gctcttatca aaaggctatg
     4441 acacgaccaa tgccaggaac aaccgtactt gctgggcaag ggaaggtggc tggcacctga
     4501 agcgatgcag acatctggca acgggcgaag atcatttctg gtttatcgca ttcaacggta
     4561 ccggcggcga catttttcct gtgaaaaccc agcaggacta ccgtgctgcc taccgcaagc
     4621 ttgaagcgta tggatatgca ccggccgtta ttgaacaatt gaacacaggc gttgcctata
     4681 atcttgctta ttcacggaca ccgttaaaac gccctgagag cgccaccagc gaactgaagc
     4741 gaaaacctga cgtgaatatt cagggtgagc cttgcgaacg ctttgtgacc gaacggagca
     4801 gcgcagcgca gaataaattc aaaaccctgc tcattgagaa cttcgccggt cggtgtgcag
     4861 tcactggatg ggttaatggt ggtgtgctgg tggcggctca tatcgagcat gggacgcgct
     4921 acaacccttc gaacggcatc ctgttgacgc cgacaatgca cgcattgttt gacgcggatc
     4981 tgatgggtat agacccttca acactcaccg ttcattttaa accgggcatc gaggcgggag
     5041 agctgtttga aggaaaaacg ctcaatccgt tggtgtatga tctggatacc gaccggctgg
     5101 ctgctcgctg ggctgacttt ataggtgccg agtgattgat gggaaattca gcctataacc
     5161 gatggcagtg ttttcgggat ggacaccact tactgatggg ggctggctgc acgccacctt
     5221 tatattgaaa tccaacagag ttgatacaac aaatgcaaaa ttgcctttcg ggatatttaa
     5281 aagcgaccat tgttatttta tctgcggacc tgacaggttg cgacctggca aatggggctg
     5341 cggaaagtgg agtgtatcag aaatacacga atggaccttt caaggaaccg actatcgtga
     5401 tgagtccggg ctggcggatg gatgaccacg gcagggaagc actaatattc gggagcagcc
     5461 actgcccgga tgtaaacggc aataatacgt ctgagggcgg ctgcgtcctc attgaggagc
     5521 actctgcgac tgtcgctgtc actgttgttg atgcgacaaa tcagtcccgt cgccaggaaa
     5581 cctggacgat tgaacgtaaa aaggatcgag ttatagtgaa gcgcccggat aacagttttg
     5641 tgatgccctg gggaaaatag ccaagataca agatgaccac aaaaatgaat aagccgcaat
     5701 gttgttttgt gtgttgaacg tcctgatgga gcctttgaca tttacactgg ccgttttata
     5761 cagttccacc aaattgacaa cagtagaaga aaaattgttg aaataagtga tcctgacctg
     5821 gtggggatga cttattcaat cccgatggtt tttaacggag ggaagttcag tggtcaaggg
     5881 gttacctgtc tctcgctccc cgcagtaaca ttaataaaag taacttttcc ttcccgaact
     5941 tatcaggagg ggcaggaaca aatcacaatg tcgagcccgg accaggcact attttctgaa
     6001 gatgtccgga aaaactgcgc cggtacgtgt gttcttaccg gcgttcgagg cagacaaaga
     6061 actgaggctg cgcacataaa accccgtcat gctggtggtg agccggatgt gacaaacggg
     6121 attctactgc gaagtgacat ccataccctc tttgacaatt ggcacttttc gatcgaccct
     6181 gattcaatga aagcccgttt cagcccggat gtgctcttcg tcgataaaga cttactacaa
     6241 ctggaaggca agcaaattga tttttcccgc ctgcaaatgc ctatcaatat tgaacacctt
     6301 cgacatcact ggaacaaatt ttttaaccgt catgtcaatc cattcataaa gaatgcgcta
     6361 aataaaaaaa tcaacaatta tgaaaaacgg ccagttaaca gcaacataca ttattaggct
     6421 atgttagcct aatggcattc tgaaagtctg cccaattaga ctgaagttca ataagaagga
     6481 atgaaaatga acttgaaaag tattgttccg aaggctgctg gccttgctgc atggattgta
     6541 ggaatcattt atagtggttt tgctctttct gttctgtggg ggtggttttt tgtgcctctc
     6601 ggtgtggtgc cgatcacaac tccatgggct attggaattt actgcttggc aggcataata
     6661 agaggcaata gagtaaaaat cccgaaagaa caagatatgc catgtacgct tcaattaacg
     6721 atgatatatg tcggcatcac ctttttccta attgtcggtt atatctgcca tcaaataact
     6781 tagaactgat ttagaattaa atttgataaa gcttactaca aagtagtttt gtcaatgttc
     6841 aacctgttta tcaaacctac ggtgactttt tatgactggg aatggtatca atactgtacg
     6901 cataaacaac gaagtgaaac acatctcaga gttagatccg gagatctctt tttcttgaat
     6961 ggaataaact gaaaagtgaa aataacgaac tgtatcgctg cataaaggaa gcaaattctg
     7021 gttggcgtgg ttttgtcctt cgtttaatag gggtttatct acctgatggc aaaacaattg
     7081 tcattaaacc ctagattatt tgaatattcc taaacatagg cttaataaga aaagccactg
     7141 aaaagtggct tttgaactga gctgttcgcg attttaattt caacttagat gaggaaatca
     7201 tccagtgttt taccggcttc aagttgttcg gccagtgctt taggggtacg cccctggcca
     7261 gtccaggttt tcgtttcacc attaacatca gtgtatttat atttcgcagg gcgcggttcg
     7321 cgaacttttt taggtgctcc ggactttgca gagaaagaac tcagtaactc ttcagggtca
     7381 attccatctt caagcattaa ttgacgcagg gattcaatct tctccagacg cgctttcagc
     7441 tcagcttctt ttgaagattc ttcctgacgt cgttcttcaa caacaacgct taatttttcc
     7501 agcagttctt caaggatctc taacggcagt tcacggccct gcgcacgaag agtacgaatg
     7561 ttgtttaatg atttgagtgc ttcggacata tgacctcact aagagtaata aataaaatca
     7621 atagaatact ttgcgatatt acactctttt attattttga gccagctaga cgcgtaagat
     7681 tttctgggcg catgatatca atcattggta cctaaagaca ttaaccaccc cgcgtatgga
     7741 aacaaatgca ttatcacttg ccagttcctc tgtagtgcca actccgcaag attagataaa
     7801 atcgccgtct ttctcatgcg attattactt tcggttactg gagagacctt ttaatattga
     7861 ttttcttcct cttgatttct tagttgtttg tccttttcgt taatatggaa agggtcgtat
     7921 gtgttggtat tataggggtc tgagggccaa tggaacgaaa acgtacgcta aggaaataac
     7981 caattgttat aattgatatt attcattctt ctctatgaac tgaaagcccg cactaagcaa
     8041 tgttttagcc tcaaatgact tttttaccta cccaactaac gtaacgatcg cacatcaggt
     8101 cgtttttcaa ccgcatagtg acacctgcac tcacatgcga gaaaacacat atgattttga
     8161 cttcttgttt tggtggagag acaataccca ttcatagcag gagtacataa tgccagaaat
     8221 tgcatcccta caacttttca aaatactatc tgatgaaacc cgcctgggta tcgtgctact
     8281 gctcagggag atgggggagc tgtgcgtatg cgatctctgc acagcgctgg aacaatcaca
     8341 acccaagacc tcccgccatc tggcgatgct tcgggaaagt ggcctgttgc tggatcgcaa
     8401 gcaggggaaa tgggttcatt atcgcttatc cccgcatatt ccttcctggg ctgccctggt
     8461 gattgagcag gcctggttaa gtcaacagga cgacgtacag gccatcgccc gtaagctggc
     8521 atccgccaac tgctctggca gcggtaaggc tgtttgtatc taaaaatttt gcctgtacat
     8581 atatgttttt acaaatatga tgtgttaaag atgaaaatgt taacggtgtt tgatccggcg
     8641 atgtgctgca gtaccggcgt ttgtggttca gatgtcgatc aggttttggt caatttttct
     8701 gctgatgtgc aatggctgaa agggcgtggg gttcagatcg aacgttataa cctggcgcag
     8761 cagcctatga gcttcgttga gaacgaaaaa gcgaaagcat tccttgaggc ttctggagct
     8821 gaagggcttc cgctgctgtt gcttgatggt gaaacggtga tggccgggcg atacccaaaa
     8881 cgcgctgaac tggctcgctg gtttggtata ccgctggaaa aggtgggttt agcttccacc
     8941 agttgctgtg gtggtaaaac ttcctgttgt tgagtatgtc aggaggacat atgaaattct
     9001 tagagaatat cccgtcttac ctgtttttca ccggtaaggg aggtgtaggc aaaacttcga
     9061 tttcctgcgc aacggctata cgtctggctg aactgggaaa gcgtgttctg cttgtcagta
     9121 ccgacccggc ttccaatgtc ggtcaggtat tcgatcagac tatcggtaac actatccagc
     9181 ccgtgactgc tgtgtctggt ctttcggctc tggagattga cccgcaggac gccgcccagc
     9241 aataccgcgc cagaatcgtt gaccctatta aaggtctttt gcctgatgac gttgttaaca
     9301 gcatcagtga acagctttca ggagcttgta cgacagagat cgctgcattc gacgagttta
     9361 ctggcttact gactgacgct tctctgctga cccgctttga tcacatcatt tttgatactg
     9421 ccccaactgg ccacactatt cgccttcttc agcttcccgg agcctggagc agcttcatcg
     9481 aaagtaaccc ggatggtgct tcctgtcttg gtccaatggc tgggctggaa aaacaacgcg
     9541 agcaatatgc tcatgcggtt gaggcattat ccgatcctga acgaactcgc cttgtgctgg
     9601 tcgcgcgatt gcaaaaatca acactgcagg aagtcgctcg tactcatgac gaattatcag
     9661 ctattggcct taaaaatcag tatttagtca ttaacggtgt cctgcctgcg agcgaagcag
     9721 aacgtgatgc actggccgct gcaatatggc aacgggagca agaggcgctg gcaaatcttc
     9781 ctgccgggtt atctgatttg ccgacagaca acttatacct gcagccgctg aacatggttg
     9841 gtgtatccgc attgaaaggc ctgctcaacg aacacgctga gataacatcg cttcctgaac
     9901 aaagcccaca gaacaagcca gagaatatgt cgttgtctgt cctggttgat gatatcgccc
     9961 gcagtgaaca cggtttgatt atgctgatgg gcaaaggtgg tgtgggcaaa accacaatgg
    10021 ctgcggctat cgccgtcagt ctggcggata agggttttga tgtgcatctc accacctcag
    10081 atcctgctgc acatctcagt acaacgctca atggcagcct caaaaatctg caggtcagcc
    10141 gaatcaaccc tcacgatgaa actgaacgct atcgtcagca tgttcttgag acgaaaggca
    10201 gggatctgga tgaggctggg aaacggctgc tcgaagagga tttacgttct ccttgcacag
    10261 aagagattgc ggtgttccag gcgttttcac gcgtaattcg tgaagctggc aaacggtttg
    10321 tggtcatgga tacggcacct accgggcata cgctattact gcttgatgct accggggctt
    10381 atcaccgtga gattgccagg aagatggggg ataaaggtca ttttaccact ccgatgatgc
    10441 agcttcagga ccaggaacgt accaaagttt tgctggtcac tctgcctgaa accacacctg
    10501 tactggaagc ggcaaacctg cagtccgatc ttgaacgtgc ggggattcat ccctggggct
    10561 ggattatcaa taacagcctt tcgattgcac agacgcaatc gccgctgctt tgccagcgcg
    10621 ccctacaaga gcgtcctcaa atcgaggttg tgaaaaacca gcatgcaagc cgcatagcgt
    10681 tagtaccggt aatggctgca gagcccactg gcatcgagaa actcagagag ctggttgttt
    10741 aatttttttt gtatatacag ggcggcaaag ccgtcctgtc tggaggattt atgttactgg
    10801 caggcgctat ttttgtcctg accatcgttt tggttatctg gcaaccgaag ggattaggga
    10861 tcggctggag tgcgacgctg ggcgcggtac tggcgctgat ctctggcgta gtccattttg
    10921 gtgatatccc tgtagtgtgg aatattgtct ggaatgcgac ggcgaccttt attgccgtga
    10981 ttattatcag ccttctgctc gatgaatcgg gctttttcga atgggcggcg ctgcacgttt
    11041 cacgttgggg taacggccgc gggcgtttgc tgtttaccta tatcgttttg ctcggcgcag
    11101 cggtggcggc actgtttgcc aacgatggtg cggcattgat tctgacgcca attgttatcg
    11161 ccatgctgct tgcattaggt ttcagcaaag gcactacgct ggcatttgtc atggccgccg
    11221 ggtttattgc cgatacggcc agtctgccgc ttatcgtgtc caacctggtt aacatcgttt
    11281 ctgcagactt ctttgggctg ggtttcactg agtatgcgtc ggtaatggtg ccggtggata
    11341 tcgcagccat tatcgccacg ctggtgatgc tgcatctgtt cttccgcaaa gatattccgc
    11401 caacttacga cctggctctc ctgaaagcac cggcaaaagc gattaaagat ctggcaacct
    11461 tcagaaccgg ctggatagtg ttgatccttc ttctggttgg ctttttcgtc cttgagccgt
    11521 tgggtatccc ggtcagtgcg attgctgctg taggggctgt cattctgttt gcggtggcga
    11581 agcgaggcca tgccattaac actggcaaag tgcttcgcgg tgcgccatgg cagatcgtca
    11641 tattctctct gggaatgtat ctggtggtct atggtctgcg taatgccgga ctaacagaat
    11701 acctctccgg tgtgttgaac gtgctggcag ataaaggact ttgggccgcg acgtttggta
    11761 ctggcttcct gacggctttc ctctcttcca tcatgaacaa tatgcctacc gtgctggttg
    11821 gtgctctctc tattgatagc agcactgcaa caggcgttat caaagaggcg atgatttttg
    11881 ccaacgtgat tggctgcgat cttggaccga aaattacacc tattggtagc ctggcaacgt
    11941 tgctctggct gcatgtcctt tcacagaaga acatgaccat cacatgggga tactatttcc
    12001 gcaccgggat cgtcatgact ctgcctgtgc tgtttgtaac gctggccgcg ctggcgctac
    12061 gtctctcttt cactttgtaa tgagatactg atatgagcaa catcaccatt tatcacaacc
    12121 cagcctgcgg cacgtcgcgt aatacgctgg agatgatccg caacagcggt atcgagccga
    12181 ccgttattct ttaccttgag actccacctt cacgcgatga gctgctcaaa ctcattgcgg
    12241 atatgggtat ttccgtccgg gcgttgctgc gtaagaacgt cgagccgtat gaggaactgg
    12301 gtcttgcaga agataaattt actgacgacc agctcatcga ctttatgcta cagcatccga
    12361 ttctgatcaa ccgtccgatc gtagtgacgc cactgggaac taaactttgc cgtccttcag
    12421 aggttgttct cgatatcctt ccagatgccc agaaagcggc tttcaccaag gaagacggtg
    12481 aaaaagtcgt tgatgattca ggtaaaagac tgaaataaag acgaaggggg catttgcccc
    12541 cttcattatt acttcgccat atcttccagc aggtcaatac caaccggctc ttcgctctgt
    12601 agcgctgtta atgcaatacg ctttgcatac tgctcttcca catcagagat gagaggcagc
    12661 tcgcggttcg ccctgatctt caggaaaggc gaagacggtt gagcagctgc caggctgtta
    12721 ttaacgaccc atgcccacgg ctcgatgcca gctcggcgta gatcctgctg caggttagtg
    12781 gcttccagta caggtgtcgt ttcggcaagc gtcacgataa tcaccttcgt cttctccggg
    12841 tctgctgctt tttcgctatc acctgtcttc gatgtcagaa agcgagagaa ttcggcttca
    12901 tgctgtcgcc agaacacact catgcaattg ttgaatgcct ttacatcact catgttcgtc
    12961 ctcaaatttt gctcagatta acccggctct catcttccag aattttataa acagtggaac
    13021 gggcaatact gagccggcgg gcaatatctg ttgcaccagt tccctgctga tgaagtgcca
    13081 gtacactatt tctgtcgatg atgcgctttc ggccaaaccg gatacctttc agcctggcct
    13141 cctgccgtcc ttcgttcgta cgctccagaa tcctcctgcg ttcggcctgg gctacagctg
    13201 aaagaatggt ca
//
LOCUS       DAOFQD010000125        13209 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.125, whole genome shotgun sequence.
ACCESSION   DAOFQD010000125 DAOFQD010000000
VERSION     DAOFQD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.125"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            167..1666
                     /gene="pitB"
                     /locus_tag="QBF46_003800"
     CDS             167..1666
                     /gene="pitB"
                     /locus_tag="QBF46_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDQ6675927.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1715..2407)
                     /locus_tag="QBF46_003801"
     CDS             complement(1715..2407)
                     /locus_tag="QBF46_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDQ6675928.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRNMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2581..3294
                     /locus_tag="QBF46_003802"
     CDS             2581..3294
                     /locus_tag="QBF46_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDQ6675929.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            3326..4084
                     /locus_tag="QBF46_003803"
     CDS             3326..4084
                     /locus_tag="QBF46_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675930.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4130..5479
                     /locus_tag="QBF46_003804"
     CDS             4130..5479
                     /locus_tag="QBF46_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDQ6675931.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSAVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5479..6303
                     /locus_tag="QBF46_003805"
     CDS             5479..6303
                     /locus_tag="QBF46_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675932.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            6315..6875
                     /locus_tag="QBF46_003806"
     CDS             6315..6875
                     /locus_tag="QBF46_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDQ6675933.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6906..7976
                     /gene="lptF"
                     /locus_tag="QBF46_003807"
     CDS             6906..7976
                     /gene="lptF"
                     /locus_tag="QBF46_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDQ6675934.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLIDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7973..9052
                     /gene="lptG"
                     /locus_tag="QBF46_003808"
     CDS             7973..9052
                     /gene="lptG"
                     /locus_tag="QBF46_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDQ6675935.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSMGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQH"
     gene            complement(9087..10259)
                     /locus_tag="QBF46_003809"
     CDS             complement(9087..10259)
                     /locus_tag="QBF46_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDQ6675936.1"
                     /translation="MGLYDKYARLAGERLQFSDNRLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10259..10507)
                     /locus_tag="QBF46_003810"
     CDS             complement(10259..10507)
                     /locus_tag="QBF46_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDQ6675937.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10539..11453)
                     /locus_tag="QBF46_003811"
     CDS             complement(10539..11453)
                     /locus_tag="QBF46_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDQ6675938.1"
                     /translation="MNQTVAVTGATGFIGKYIINNLLARGFHVRALTRTARAHVNDNL
                     TWVCGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLATNVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11450..13180)
                     /locus_tag="QBF46_003812"
     CDS             complement(11450..13180)
                     /locus_tag="QBF46_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDQ6675939.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHDNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIELLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         3178 a         3262 c         3425 g         3344 t
ORIGIN      
        1 acctgtgttt tgattatttc taaaggtttt gaatgaatgc ttattgtctg atacacaaga
       61 aataacactc tttttatcgt taaaaaatga tatttcactt tgcccatgcc gttaaagtca
      121 ctgataatgc gtccgttcgt aaattcaaaa tggcgtaatc taatatatgc taaatttatt
      181 tgttggcctt gatatataca cagggctttt gttattgctt gctctggcat ttgtgttgtt
      241 ctacgaagca atcaatggtt ttcatgacac ggcgaatgcg gtggcaaccg ttatttatac
      301 tcgtgccatg caaccacaac ttgctgtggt gatggcggca ttttttaact ttttgggcgt
      361 gttattgggc ggacttagcg ttgcctatgc cattgtccat atgttgccaa ccgatttgtt
      421 gctgaatatg gggtcaaccc acggcctggc gatggtcttt tccatgctgc tggcggcgat
      481 tatctggaac ctgggaacgt ggttcttcgg tttaccggcc tccagttcgc acaccttgat
      541 tggtgcgatt atcggcatcg gtttaaccaa cgcgctgtta accggctcat cggtgatgga
      601 tgcgttaaac ctgcgtgaag tgaccaaaat tttctcctcg ctgattgttt cccctatcgt
      661 cggcctggtc attgcgggag gcctgatatt cctgctgcga cgctactgga gcgggacgaa
      721 aaagcgtgac cgtattcacc gcattccgga agatcgcaaa aagaaaaaag gcaaacgtaa
      781 accgccattc tggacgcgta ttgcgctgat tgtttccgct gcgggcgtgg cgttttcgca
      841 cggcgcgaac gacggacaaa aagggatcgg cctggtaatg ctggtactgg tggggattgc
      901 ccctgctggc ttcgtcgtca atatgaatgc gtccggctat gaaattaccc gtacccgcga
      961 tgccgttacc aacttcgaac actacctgca acagcatcct gaactgccgc agaagttgat
     1021 tgcgatggaa cctccattgc ctgcagcatc gactgatggc acgcaagtaa cagagtttca
     1081 ctgtcatccg gcaaatacct ttgatgcgat tgcgcgcgtt aaaacgatgc tgccaggcaa
     1141 tatggaaagt tacgagccgt taagcgtgag tcagcgcagc cagctgcgcc gcattatgct
     1201 gtgcatctct gatacctccg cgaagctagc gaaactgcca ggcgtcagta aagaagacca
     1261 gaacctgctg aaaaaacttc gcagcgatat gttaagcacc attgagtacg ctccggtgtg
     1321 gatcatcatg gcggtagcac tggcgctcgg cattggcacc atgattggct ggcgtcgtgt
     1381 agcgatgacc atcggtgaga agattggtaa gcgcggcatg acgtatgcgc aaggcatggc
     1441 ggcacaaatg acggcggcag tgtctatcgg tcttgccagt tatattggga tgcccgtctc
     1501 cacaacacac gtcctctcgt ctgcagttgc agggacgatg gtggtggacg gcggtgggtt
     1561 acagcgtaaa acggtaacca gcatcctgat ggcgtgggta tttactttac cggcggcaat
     1621 ttttctttct ggtgggctgt actggatagc attgcagttg atttaacctt cctgaaaatg
     1681 cccggtcctg gcgatcgggc atttccattt ttgactagtg ataaccacgc gcggtcataa
     1741 aatccgtaat cgctttttct gcatcaacca acacctgttc cagtggctga ttggcatcga
     1801 tatcaaccag ttgtgcacca ccaaaggtta actgtggcgt tatggcaatc ttcctcgcca
     1861 gcgattcccg tttatggtcg ggtttacgtg cacaggcaac ttcaaggtca acattgagtt
     1921 tgatgaccag atcaggctta tggctcgcca tccagtgaaa cgctttacgt tcctggcttg
     1981 ccagccatga gacaaaacga ccaccttcaa cgttaggtgg gaacaccgta ccatcgtaag
     2041 cgccaggaat ttggtcctga ggataacggt cggttagaac aattaacccg cgacgacgac
     2101 aggcaagcat attacgaaag cgcagtaagc gacgggcgac aaacgctgta attaccagcg
     2161 ccggaactgg tccaggcaat ttttttgctg ttttcacctg atttcgttca attgttttat
     2221 gtaaggattt tcccatcaac ggtaattttg tcactgcacg accgacattt ccggcctgtt
     2281 ttcctaaatg aactctttcg gcagcaccat atttttcgac aacggtaata agatgttcac
     2341 acaccgttga cttgcctgaa ccatcgctac caataacggc aattaatgga ggtgttattg
     2401 actgcatgaa tatatccttt aatttaaata tccattaaaa atatttattt ggttaatatg
     2461 tttttatgaa agcgtaattc aggtcaatgt cacaattaac cattgtcaca ataaggttga
     2521 acggatattc tgagcacgga ccacgtaata ttcaatacat tatttactgc cgggtttttc
     2581 gtgaatcaac gtaatatgtc aataattaat tccacgccag tgcgtgttat tgccattgta
     2641 ggatgtgatg gttcaggtaa atcgaccctc acggcaagcc tggtaaatga actggcagca
     2701 agaatgccaa cagaacacat ttatctcggg caatcgtccg ggcgaattgg cgaatggatt
     2761 tcacagctcc ctgttattgg cgcacctttt gggcgttatc tgcgaagtaa agcggcacat
     2821 gtgcacgaaa agccctcaac accgcctggc aatattactg cactggttat ctatctgctt
     2881 tcctgctggc gggcgtacaa gtttcgcaaa atgttgtgta aaagccagca aggctttctg
     2941 ctcatcaccg accgctaccc gcaggttgaa gtgccggggt ttcgctttga tggcccgcaa
     3001 ttggcaaaaa ccacgggcgg taacggttgg ataaaaatgt taaggcagcg cgagctgaag
     3061 ctgtaccaat ggatggcatc ttatttgccc gtattgttga ttcgtcttgg cattgatgaa
     3121 caaaccgcgt ttgcgcgtaa acctgaccac caactggcag cgttacagga aaaaatcgcc
     3181 gttacgccgc aactgacatt caatggcgca aagatccttg aactggatgg gcgacacccc
     3241 gccgatgaaa ttctgcaagc gtcactacgc gcaattcacg ccgcgctttc ctgatcacca
     3301 aaatggatag gggatgattt aacgaatgga tgcactacaa actcaaactg ttaatagtac
     3361 aaccgcgccg cagcccaact acattccggg gctgattgcg gtggtcgggt gtgatggcac
     3421 cggtaaatcc acactgacca ccgacctggt gaaatcgctg caacaacact ggcaaaccga
     3481 gcggcgctat ctggggctgc tctccggcga agacggcgac aaaatcaaac gattgccgtt
     3541 ggttggcgtc tggctggaac ggcgactggc ggccaaatcc tcgaaaaccc aaagcatgaa
     3601 aaccaaatct ccggcgctat gggcggcggt gattatgtac tgcttctcgc tgcgaagaat
     3661 ggcgaatcta cgcaaggttc agcgactggc gcaaagtggc gttctggtgg tcagcgatcg
     3721 cttcccgcag gctgaaattt cgggctttta ttatgatgga ccggggattg gcgtcgaacg
     3781 tgcgaccggg aaaatcagca tgtttctggc gcagcgcgaa cggcgtttat accaacaaat
     3841 ggcgcaatat cgcccggaat taattattcg cctgggcatt gatattgaga ctgccatctc
     3901 ccgcaagcct gaccatgact atgccgagct gcaggacaaa atcggtgtca tgtcgaagat
     3961 tggctataac ggcacaaaaa ttcttgagat tgattccaga gcgccctaca gcgaagtgct
     4021 ggaacaggca cagaaggcgg tttctctggt tgccatcgtt tctgaccgcc gaagtttaac
     4081 ttaaggctgg cgggatactt tttactatca acgataaggt caggctgaat tggcgggttt
     4141 taacatcaaa cattggtttg cagatggcgc gtttcgcacc attattcgca atagcgcctg
     4201 gttaggctcc agtaatgtcg tgagcgcttt gctgggtctg ttggcactct cgtgtgccgg
     4261 taaagggatg acgcccgcca tgtttggcgt actggtgatt gtgcaatcgt acgccaagtc
     4321 gatcagcgat tttattaagt ttcagacatg gcagttggtg gtgcagtacg gaacgccagc
     4381 cttgaccaac aataatccgc agcaatttcg caatgtcgtc tcattttcct tctcgctgga
     4441 tatcgtcagc ggcgcggtgg cgattgtcgg cggcattgcc ttactaccat tcctttccca
     4501 ttcattaggt ctggatgacc aaagtttttg gctggcggcg ctgtattgca cgctcattcc
     4561 ttcaatggct tcctccacgc cgaccggcat tctgcgtgcg gtagatcgct tcgatttaat
     4621 tgctgtacag caggcgacga aaccttttct gcgcgcagcg gggagcgtcg tagcctggta
     4681 ttttgacttt ggttttgcgg gttttgttat tgcctggtac gtgtcgaatc tggttggcgg
     4741 caccatgtac tggtggtttg ccgcgcgcga attacgccgc cgaaatatcc ataacgcctt
     4801 caaattgaat ctgtttgagt ctgcccgaca cattaaaggc gcgtggagtt ttgtctggtc
     4861 aaccaacatt gcccactcca tctggtcggc gcgtaactcg tgcagcgcag tgttggtggg
     4921 gatcgtgtta ggacccgctg ccgccgggtt atttaaaatc gccatgacat tcttcgacgc
     4981 cgccggaacg ccagcgggtt tgctgggtaa aagtttttac ccagaggtga tgcgtttaga
     5041 tccgcgcacc accagaccgt ggttgttagg tgtgaagtcc ggtttactgg cgggcggaat
     5101 cggtattctg gtggcgctcg cggtgttgat cgtcggcaag ccgctcattt cgctggtgtt
     5161 tggcgttaag tatctcgaag cgtatgacct gattcaggtg atgctgggcg caattgtgat
     5221 ttcgatgctg ggcttccctc aggagtcatt gctattgatg gcgggaaaac agcgcgcttt
     5281 tctcgtggcg caaaccatcg cctcaatcgg ctacatcgta ttgctgttca tgttctgtca
     5341 tctgtttggt gtgctgggag cagcgtttgc ttacttcggt gggcaatgtc tggacgtggc
     5401 gctctcgtta attccaacct taaaggcatt ttttcagcgc cattccttgc tttataacgc
     5461 agccggagag aaatcctgat gatgagaaaa tatttcccgc ttgaggcgag cgagcgtcta
     5521 tttgttgcga ttgaagaaga tgatgtcgtt gatgcacagg ttagtttgcc gccgactatt
     5581 gcactaagtt gtaccaccga aatcattcat gataattacg cgttatgcct gcaattctgg
     5641 ctcaatggcg ttgatcgtca ggaattactg cgtttagttc gcaaacaagc caaaggcgat
     5701 gagctgactg ctgatgagcg caaacaattt aaatacatgc gagcgcgcta caagcacctg
     5761 cgttttgcgc aacggctgta tttaaagaag catcaggccg gatttttatt cggcaaaacg
     5821 accgtttttc tggggcgttt tcaggacggc tttcgtaacg gtaagaaaaa tattgtgtcg
     5881 tattacggca acctgttacg gatttattta agctctccgg tctggtcgtt ggtgaactat
     5941 tcattacgcc atagccagtt agaaagcgta agcagtttta ttgcctatcg gcaaaagcaa
     6001 atgcataccc tgaaagagat tattgccaaa ccgcgactaa ccggtaggga atttcatgat
     6061 gtgcgaaaaa tcatcagcca gcaggtttct tattacgaca ccttaagatc gcttgatcct
     6121 gaaaataaag aggcgttaca gatatcccgc ttcctcgccg ccattaatgg cctgatggga
     6181 gataaacacg acgacatggt ggcggatgat atggaaaacc gccagtcgta tgatgcaccg
     6241 gtggcgctgg atagcgatat tcgtcagcgc ttagagctgc tgatttcgcg ttttccgcta
     6301 tagccggagg aatgatgtca tctcgtttga ttattgcgtt gattatcatg ctcttagccc
     6361 cgggtgtgca ggcacacaat ttcgtcactg gcaaaacggt tacccctgtt tatatccagg
     6421 aaggcggcga gctgcttctg aatagcgacg acgaaattca ctaccaaaag tggaacagca
     6481 cacaattagc gggaaaagtc cgcattattc agtatatcgc cgggcgtaag tcggcgaaga
     6541 aaaagaactc actgttaatt aaagcggttg aagcggcaaa ctttccgcag gatcgttttc
     6601 agcccaccac cattgtgaac accgatgatg ccatctttgg tactggctat tttgttgttg
     6661 gcaaaatcga aaaaaataaa cgccgctatc cgtgggcgca attcgttatt gacggcaatg
     6721 gacagggacg cgttgcctgg cgcttgccgg agcagagttc aaccattctg gtgctgaata
     6781 aagccgggca aattcagtgg gcaaaggacg gcagcctgac gccggaagag gtagatcatg
     6841 tcattgcgtt ggcgcagaaa ctgatcaatg aataaaaaac tcaaaggatt gagggcgtta
     6901 gtgagatgaa gctggttgaa cactacatca tgcgtggtac gcgccgtctg gtgctgatta
     6961 tcgtcggttt tctgatcttt attttcgcca gctactccgc acagcgttat ctgaccgaag
     7021 cggcaaatgg gacattagct cttgatgttg tgctggatat cgttttctac aaagtactga
     7081 ttgcactgga gatgttgtta cctgttggtc tgtatgtgtc agttggcgtg acgctagggc
     7141 agatgtatac cgactcggaa attaccgcta tctctgcggc ggggggcagt ccgggacgct
     7201 tgtacaaagc cgttctttat ctggcgatac cgctaagtat ttttgtcacc cttctgtcga
     7261 tgtatggtcg tccgtgggct tatgcgcaga tttatcaact ggagcagcag tcgcagtcgg
     7321 agctggatgt tcgccagttg cgggcaaaga aatttaacac taacgataac ggacgaatga
     7381 tcctttcgca gacggttgat caggataata atcgcctgac tgacgcgctg atttatactt
     7441 ctactgccaa tcgaacccgc attttccgcg cccgttcggt tgatgtggtt gacccatcac
     7501 ccgagaaacc gaccgttatg ttgcacaacg gcactgccta tcttatcgat catcaggggc
     7561 gtgatgacaa cgaacagatc taccgtaatc tgcaattaca tctgaatccg ctggatcaaa
     7621 gccctaacgt caaacgcaaa gcgaaatcgg tcacggagct ggcgcgctcc gtctttccag
     7681 ccgatcatgc cgaactgcaa tggcggcaaa gccgtggcct gaccgcattg ttgatggcgc
     7741 tgctggccat ttcattaagt cgggtaaaac cgcggcaagg gcgattttca acgttattgc
     7801 cactgacgtt gctgtttatt gccatttttt atggcggtga cgtctgccgt acgctggtgg
     7861 ctaacggcgc gattcccctc attcctggtt tgtggttagt acccggactc atgctaatgg
     7921 gcctgctgat gctggtcgca cgcgacttct ctttgctgca gaaattttcc cgatgaatgt
     7981 tttcagccgc tatttaatcc gtcatctctt tctcggtttt gccgccgccg cagggctatt
     8041 gctgccgctt tttaccacct tcaacctgat taacgaactg gatgatgtca gcccgggcgg
     8101 ttatcgctgg actcaggcgg tgctggtggt gctaatgacc ttaccgcgca cactggtcga
     8161 actttcgcca tttatcgcct tattgggagg gattgtcggc ctggggcagt tatcgaaaaa
     8221 cagtgagctt accgccattc gcagcatggg gttttctatc ttccgtattg cactggtggc
     8281 gctggttgca gggatattgt ggactgtttc gttaggcgcg atagatgagt gggtagcgtc
     8341 gccattacag caacaggcgt tgcaaatcaa atcgaccgcc accgcgttgg gggaggacga
     8401 tgacattacc ggcaatatgc tgtgggccag gcgtggtaat gaatttgtga cggtgaaatc
     8461 gctgaacgag cagggccagc cggtgggcgt ggagattttt cattatcgcg acgatctctc
     8521 gctcgaatcc tacatttatg cacgcagtgc caccattgaa gacgacaaaa cgtggatcct
     8581 gcatggtgtg aatcataaaa aatggctgaa tggcaaagaa acgctggaaa cattagataa
     8641 tcttgcctgg caatcggcct tcaccagtat ggatcttgaa gagttatcaa tgccgggcaa
     8701 tactttttct gtccgtcagc ttaatcatta catccattat ttgcaggaaa ccgggcaacc
     8761 cagcagcgaa taccgccttg cactgtggga aaaactgggg caacccatcc tgaccctggc
     8821 gatgattttg ctggctgtgc cgtttacctt tagcgccccg cgctcgccag ggatgggtag
     8881 ccgtctcgct gtaggtgtca tcgttggctt actcacctgg atcagctatc aaatcatggt
     8941 caatctggga ttgctatttg cgttgagcgc acctgttacc gcgctcggtt taccgatagc
     9001 gtttgtgctg gtggcgttga gcctggtgta ttggtatgac agacaacatt aaacccgccg
     9061 tggcgcaaca ccacggcggg ctcacattac tgcttcagag tagcaaatgc ctgaatgatg
     9121 gcatcgattt gctcacgact gtgagcagcg tttacgctac agcgcagcag cgtgatgcct
     9181 gcaggcgcgg caggtggcag gataagattc acataaacgc ccagcgaaat aagctcgcgc
     9241 cagatgcgta aaccgtcctc tttcgaaccg attattaccg gcactaccgg gctaatatgt
     9301 gaacttaact cataacccag tttttgcaga ccgtcataca gatggttggc attatccatc
     9361 agcttttgcc gcagctctgg ttgtgtggca atagttctta acgacgagcg cacggtcgca
     9421 atgcaggacg gagaaggaga ggcggtaaaa atatacgggc ggctgccgta gcgcagcact
     9481 tccatcgctt ccgaaccgac agcaaaaccg ccaatcgagg ccagactttt actgaaggtg
     9541 ccaacgataa tatccacatc atcttcaacg ccgacagctt cagccagtcc gcgccccgtt
     9601 gcgcctaaca cgccaaacga atgcgcttca tcgacaatca aataaccacc gagacgacgt
     9661 ttgatatcaa caatttccgc taacggcgca acgtcaccga gcatgctgta gataccctca
     9721 acgatgataa tcgcctcttt cgcccgttca ccgaggcgaa ccatgcgccg ttccagatct
     9781 ttagcatcgt tatggcggaa acgaataatt tccgcgccac cgagagaaca ggcgtcataa
     9841 atactggcgt ggctgtcggc atccagtaac accacggcgt tatggtcggc aagtgcgctg
     9901 atgacgccta aattagcggt ataaccggtt gaaaaaacga tagcggtagg gcgattgaaa
     9961 aactcggcaa tctctttctc taacgccagg tgcggcgcat aactgccgtt tgccatacga
    10021 gaaccggtgg tgccggtgcc ttgggccgct aacgctgcct gaccttcggc gatggcatca
    10081 tgattaaagg ttaaaccgag gtaattattg gtcccggcca gaatcacttt tttattgccg
    10141 atccggcctt cggtggcgga atacacttca tcaatacagg tgccaaacgg tgttaacctg
    10201 ttgtcactaa attgcagtcg ctcgccagca aggcgcgcat atttatcgta tagccccatt
    10261 atttattctc cagccatgga agtagagttt ccattaattg cgcaggggtt ttgacatcca
    10321 gcagaatatt aataggaatg gaaatatcga atctgtcttc cagcatcatt aagagatcca
    10381 tcactttgat cgactccagg ccaagatcgt tgacgagatc gctgtcgggc ttaatttcca
    10441 caccgttttc gaccaaatcc tgtaaacagg aaaggatgta atccattact atttcacgat
    10501 ttaccataga aacgttaaca cacctttaaa caagacaatc aaaatagccc gttgcgtaat
    10561 gcgtgttcca ggctaatccc gggaaaccaa ttaatatctt cagaaatacg gttattactt
    10621 gccgaccagt cggcgtgggt taattcacga attttcgagc gggtcagcat aggttcttta
    10681 cctgccaggc gactcaacgc ggtactgata tccgcaaggc aggtgaggac cggcagagga
    10741 ataccaacca ttcgcacgga accgcaacgg acgttggtgg caagttgctg tatgcgttgc
    10801 cagtcatagc cgccagcgac gccatcgcat aattcatagg tttgcgtctg tacagtttcg
    10861 gcgcttaacc actgacccac tgcttgcgca aaatcggtga cgtgcaggaa agagagctgt
    10921 gtatctggtg caccaagtcg tggcagcagg ccgcgcagca tccagtcaaa cagcggtttt
    10981 aactctttat cgccgggacc atacacggct gtcgggcgaa aaacgcccag cgtaatttcg
    11041 tcagccattg cagtcagccg ttgttcggcg acgtgtttgg aatttgcgta ccaggagagc
    11101 tcgggatggc gcgccgccag cgaagagata aacagaaaac gttggcaaaa gccgctctct
    11161 tttgctgctt gcatcaggcg caggctgccg tcaacgttac agtgggtaaa aatctcttct
    11221 ttgtgtccgc gcacttgccc ggcgcaatgg accaccgcgc tggctccggc aaccagttcg
    11281 ctaagcgaat gtgtatcttc cagcgaaccg cacacccaag taagattatc attgacgtga
    11341 gcgcgggcag tacgcgtcaa tgcgcgaacg tgaaagccgc gggcgagcag gttattgata
    11401 atatatttac cgataaaccc ggtagcgccc gtcaccgcga cagtttgatt catgccaggg
    11461 attcctgcac atgaagactg gcagcataag ccttctgata acgttttttc gcttccgcac
    11521 gggcaggctt gccggaggac gttcggggaa tactgtgggg cggcaacagc tcgatagccg
    11581 cggtcacgcc aaattcgctt tggatccgtg ccgccagcgc gtggataagc tgcccgcgac
    11641 gttcttcgtc gctgatccga cactggatct gcaaaatgat tttttcctgg gcggtaacaa
    11701 aagcaatcgc atcgccagaa tgaatttccg gttcttgttc cgcaatatat tcaatatcct
    11761 gcggccagat attacggcca cgaataataa tcagatcttt aatgcgtccg gtgacataca
    11821 gataaccgtc cagcagataa ccgaggtcgc cggtgtctaa ccagcccgtc gcggcaatct
    11881 cgtcttgcga agcctggtcg ccaaagtaac cgctcatcag actgggaccg gagatgcaaa
    11941 tatggcctac cacacgttcc gcgaccggca tacctgcttc attgcggatt tcaataccat
    12001 gttccggcaa cgctttgccg cagttgacga aagtcgatac ggcgcgtgtc tctgcacccg
    12061 gcgcgacggc tttaccctga tattcgagga tgtcgcgatc cacttcgtta accacaaccc
    12121 cggaggcttc atcagagaag ctgacagcca gcgcattttc tgccagtccg tagcacggca
    12181 tgaaagtttt attgtcaaag ttaacctgac ggaaacattc agcgaattga tggagttgtt
    12241 ctgcggagat gggttctgca ccaataccag cgacgcgcca gcaggaaaga tccagttcag
    12301 cgagatcttt ttcattcacg cggcgctggc acaattcata gccaaacggc ggcgcaacgg
    12361 aaacggtgcc gcgattttta ctgatcaatt taagccattg cagaggacgc atggcaaaat
    12421 cctgagtgcg caaataatct actgaaagct gcgtggcgac gggggtcagg agaaagccga
    12481 ccagtcccat atcatggtag aaaggcagcc aggagacgca gcggtcgcca gggcgtaatt
    12541 taatgccgtc gtggcttata gcacgtagat tagccatcac ttcgcgatgg gtgataatga
    12601 cgccacgggg aaaacgggtg ctgccggagg tgtactggag gtaggcgata tcgttcggaa
    12661 ctggacgctg gagcgcaaca tcggcttccg gtaatgcctt aaaccaggcg tggcttaaaa
    12721 catgtaattc ggggttgtca tgcgtcgcgg cattgaccag tggcaaccac tcatcaccag
    12781 tgataatggc tgcgggctgg caacttgcca gtaaaccctg caatttggcg ctccaggaat
    12841 cccgctgacc aacgcccatt ggaatcgcca acgggacggc gactaagccg gcatactggc
    12901 aggcaaaaaa agcctctacg aacccgctac ttgtttcggc aatcagtgcc acgcgatcgc
    12961 cttttttcag gttcagcgat aacaaccgct tcgcaccagc ttcggcacgt gcttttaacg
    13021 tctgatattc cagttgatct tcaagttggc aacgtctgtc ataaaaattc attccggcgc
    13081 tactcagagc ggcgtagtcc aaagcatcaa ccagcgttgg aaaatcggca tagcgcatag
    13141 gtagggaatg cgtaaagatt ttattagaca tatacaccac gaaaaaattg gatatccaaa
    13201 ataaaatga
//
LOCUS       DAOFQD010000126        13199 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.126, whole genome shotgun sequence.
ACCESSION   DAOFQD010000126 DAOFQD010000000
VERSION     DAOFQD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.126"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..265
                     /locus_tag="QBF46_003813"
     CDS             <1..265
                     /locus_tag="QBF46_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDQ6675940.1"
                     /translation="VRRTVTMLPSFIVILMGLDPTRILVMSQVLLSFGIALALVPLLI
                     FTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
     gene            complement(406..732)
                     /locus_tag="QBF46_003814"
     CDS             complement(406..732)
                     /locus_tag="QBF46_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDQ6675941.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(847..2103)
                     /locus_tag="QBF46_003815"
     CDS             complement(847..2103)
                     /locus_tag="QBF46_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDQ6675942.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            2307..3272
                     /gene="glk"
                     /locus_tag="QBF46_003816"
     CDS             2307..3272
                     /gene="glk"
                     /locus_tag="QBF46_003816"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDQ6675943.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            3491..3817
                     /locus_tag="QBF46_003817"
     CDS             3491..3817
                     /locus_tag="QBF46_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDQ6675944.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            3839..5086
                     /locus_tag="QBF46_003818"
     CDS             3839..5086
                     /locus_tag="QBF46_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDQ6675945.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWETSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            5101..6186
                     /gene="ypdF"
                     /locus_tag="QBF46_003819"
     CDS             5101..6186
                     /gene="ypdF"
                     /locus_tag="QBF46_003819"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDQ6675946.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            6186..7223
                     /gene="ypdE"
                     /locus_tag="QBF46_003820"
     CDS             6186..7223
                     /gene="ypdE"
                     /locus_tag="QBF46_003820"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDQ6675947.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWVETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            7248..9743
                     /gene="ptsP"
                     /locus_tag="QBF46_003821"
     CDS             7248..9743
                     /gene="ptsP"
                     /locus_tag="QBF46_003821"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDQ6675948.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGPLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRMIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGVAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLMLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPKFAGLFRTQLRAILRAASYGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9746..10603)
                     /locus_tag="QBF46_003822"
     CDS             complement(9746..10603)
                     /locus_tag="QBF46_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6675949.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(10616..11350)
                     /gene="ypdB"
                     /locus_tag="QBF46_003823"
     CDS             complement(10616..11350)
                     /gene="ypdB"
                     /locus_tag="QBF46_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDQ6675950.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(11365..13062)
                     /gene="ypdA"
                     /locus_tag="QBF46_003824"
     CDS             complement(11365..13062)
                     /gene="ypdA"
                     /locus_tag="QBF46_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDQ6675951.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3098 a         3407 c         3605 g         3089 t
ORIGIN      
        1 ggtgcgtcgt acagtcacca tgttgccgtc atttattgtc attctgatgg gattagatcc
       61 gacacggatt ctggttatga gtcaggtact gttaagtttt ggtatcgctc tggcgctggt
      121 tccactgctg attttcacca gtgacagcaa gttgatgggc gatctggtga acagcaaacg
      181 cgtaaaacag acaggctggg tgattgtggt gctggtcgtg gcgctgaata tctggttgtt
      241 ggtggggacg gcgctgggat tgtagttgaa tgagcgtcgc atctggcact attggcggat
      301 gcggcgtaaa cgccttatcc gccctacgcg ttaaagagcc ggtttgtagg cctgataaga
      361 cgcactagcg tcgcatcagg cattgtgctc cattggcgga cgcgtttaat ggcggtgccc
      421 atgaccccgg ccttttccgc gatgatcgtc acgatcgcgc cagccttcac gatagccacg
      481 ctcatacgct ttacgcttat cccagccacg gtggtagcca ttatcatgac gccaccaacg
      541 gtttttgcgc cactcataat tgcgatgcca gtagtcacgg tcgcgccagt gaccaccgtc
      601 ccagtaatta ccgtaatgat cgcgatcgcc aatttgtaat ttgattgatg gcagtagggt
      661 gatttcacct gcgtttgctg caagcggggt aaatgccatc agggcggccg ccagaaacag
      721 tgacctgaac attgttattc tccttcacgc tcgaagccgt cagcggcctg ttaacgcaat
      781 attacgggga ggtaaagccc cgtatcatcg ccataactct taaatcataa aggagagcat
      841 tttttgctaa acgttatcgt ggggtggctg ttgcttcgga cgattgacca tcatcatcgg
      901 cttacctgct aataacagcc atgccggaag catgacgata cagagcagcg gtagcaatgt
      961 ggtattgggt acaacgactg ccgccataaa aagacttaac cagccatcgc gtgttaccac
     1021 cagcacgatg ccgagaatag cgcaagaaac ggttattgcc gctggtacgg cgggaacgtg
     1081 ctcatgcagc atcaacccta atgccacgcc gacaaacact gccgggaaga ttcgcccacc
     1141 gcgaaagcca ctggcggcag caacgaccag ggcggcaagt ttaattaccg ccagcaaaaa
     1201 gtaatcgctg gtgctgaaag cctgatttgc caccatctgc tgcatctcat ccagcccttt
     1261 aaacagcgaa actggtccac caataacccc cagaataccg agaataaatc cgccaatacc
     1321 cagcacgagc accggatttt tcatttgatg catcatcgcg tgcaaccgtg gtaagcacca
     1381 tacggcgacc atccctgctg cgatggcaat cgccgcgaca attgcaccgc tgagaatatc
     1441 ggtcatttcc atctgtccgt aatgagcaat gggcagtgaa aaatgaggat ggaaaaataa
     1501 tccggtagta agtgcaccag ctgctgccgc cattaacggc gcaaagagac gatcccatag
     1561 cggaacttca ctactgccat ttaaggtttg cgaaaatatc aacgccgccg caacaggagt
     1621 gccaaacagt gcgccgatgg ttccggcaga ggctaaaata gtccactcca ttcggttgac
     1681 gcgcggtaac agacgagcgc caatggcaac cgcaagggcg atattgacgg tcatgatcgg
     1741 atgttccggc cccaggctga cgccgccagc aagaccgaga attaatgcta cgataagtcc
     1801 aggtagcgca gagggcggaa ccggtgcgcc gatcagcggt tcacaggcgg ggtctggtcc
     1861 ggcatgaccc tggctgaaac ggataaccaa ccccaccgca atacccgtta gcgttaatac
     1921 accgatgatc cagaggggtg aatcctgggc tatccccaga gttcccggca gtcgttgcca
     1981 gagcaaattc tgtaataccg aggcgatttt catcaccaca atcagaataa gactggacgc
     2041 aatcccaatt gccacggcgg ggagcgataa taacaacatg gttctggctc gcggatggag
     2101 catgttctat tccttatgcg gggtcagata cttagtttgc ccagcttgca aaaaggcatc
     2161 gctgcaattg gtgctgaaac gataaagtaa ttgtgtgacc cagatcgata tttacaggga
     2221 gcctgccttt ccggcgttgt tgttatgccc ccaggtattt acagtgtgag aaagaattat
     2281 tttgacttta gcggagcagt tgaagaatga caaagtatgc attagtcggt gatgtgggcg
     2341 gcaccaacgc acgtcttgct ctgtgtgata ttgccagtgg tgaaatctcg caggctaaga
     2401 cctattcagg gcttgattac cccagcctcg aagcggtcat tcgcgtttat cttgaagaac
     2461 ataaggtcga ggtgaaagac ggctgtattg ccatcgcttg cccaattacc ggtgactggg
     2521 tggcgatgac caaccatacc tgggcgttct caattgccga aatgaaaaag aatctcggtt
     2581 ttagccatct ggaaattatt aacgatttta ccgctgtatc gatggcgatc ccgatgctga
     2641 aaaaagagca tctgattcag tttggtggcg cagaaccggt cgaaggtaag cctattgcgg
     2701 tttacggtgc cggaacgggg cttggggttg cgcatctggt ccatgtcgat aagcgttggg
     2761 taagcttgcc aggcgaaggc ggtcacgttg attttgcgcc gaatagtgaa gaagaggcca
     2821 ttatcctcga aatattgcgt gcggaaattg gtcatgtttc ggcggagcgc gtgctttctg
     2881 gccctgggct ggtgaatttg tatcgcgcaa ttgtgaaagc tgacaaccgc ctgccagaaa
     2941 atctcaagcc aaaagatatt accgaacgcg cgctggctga cagctgcacc gattgccgcc
     3001 gcgcattgtc gctgttttgc gtcattatgg gccgttttgg cggcaatctg gcgctcaatc
     3061 tcgggacatt tggcggcgtg tttattgcgg gcggtatcgt gccgcgcttc cttgagttct
     3121 tcaaagcctc cggtttccgt gccgcatttg aagataaagg gcgctttaaa gaatatgtcc
     3181 atgatattcc ggtgtatctc atcgtccatg acaatccggg ccttctcggt tccggtgcac
     3241 atttacgcca gaccttaggt cacattctgt aaatccttcc ttttatatcg ggaggtaact
     3301 ctcccgataa tcttttaaat catacagttt attcaatttt tctttgtgtc ccctcacaag
     3361 gccgacctgc gtcacacttc cgtacagcgg gattaattct ccagtaaatg cattatttgt
     3421 ctggtaacgg cgatttgttt tgcacgttca taatttcact cgtcaactat cactgaacga
     3481 ggaattaacg atgagtaaga aactgattgc cttatgtgcc tgcccgatgg gcctggctca
     3541 cacctttatg gccgctcagg cgctggaaga agcggcggta gaagccggtt atgaagtgaa
     3601 aattgaaacc cagggcgcgg acggtatcca gaatcgcctg acggcgcagg atatcgccga
     3661 agcgaccatc atcatccact ccgtggcagt taccccggaa gataacgaac gtttcgaatc
     3721 acgcgacgtt tatgaaatca ctttgcagga cgcaattaaa aacgctgcgg gcatcatcaa
     3781 agaaatcgaa gagatgattg cctctgaaca acagtaatat tcgacaggga taattgttat
     3841 ggccattaaa aaacgcagtg caaccgttgt gcctggcgca tccggtgcgg cagcggcagt
     3901 taagaatccg caggcctcta aaaccagctt ctggggtgaa ctcccgcagc atgtgatgtc
     3961 agggatttca cgcatggtgc cgaccttaat tatgggcggt gtgatcctcg ctttcagcca
     4021 gctgattgct tatagctggc ttaaaattcc tgctgagatc ggcatcatgg atgctcttaa
     4081 tagcgggaaa ttctccggtt tcgacctctc cttacttaaa tttgcctggc tgtcgcagtc
     4141 cttcggcggc gtgttgtttg gttttgccat tccgatgttt gctgcttttg tggcgaactc
     4201 tatcggtggc aaactggcgt tcccggctgg ttttatcggc gggttgatgt ctacccagcc
     4261 gacgcaactg ctgaacttcg atcccagcac tatgcaatgg gagacctctt cgccggtgcc
     4321 gtccaccttc attggtgcgc tgattatttc tatcgtcgcg ggttaccttg tgaagtggat
     4381 gaaccagaaa atccagttac cggatttcct gttagcgttc aaaaccacat ttttgctccc
     4441 gattctttcc gccatttttg tcatgctggc gatgtactac gtcatcaccc cctttggtgg
     4501 ctggatcaac ggcggtatcc gtactgtgct gactgccgca ggtgagaagg gcgcgctgat
     4561 gtacgcaatg gggatcgccg cggcgacagc aatcgacctt ggtggcccga tcaacaaagc
     4621 cgcaggtttt gttgccttca gctttaccac tgaccacgta ttaccggtca ccgctcgttc
     4681 tatcgctatc gttattccgc cgattggtct ggggctggcg accattatcg accgtcgttt
     4741 aaccggcaaa cgcctgttca acgctcaact ttatccgcag ggtaagaccg ccatgttcct
     4801 ggcgtttatg gggatcagtg agggtgcgat tccgtttgcg ctggaaagcc ccatcaccgc
     4861 cattccgtcg tatatggtcg gcgctattgt cggctcaacc gccgctgtct ggctgggcgc
     4921 agtgcaatgg ttcccggagt ccgctatctg ggcatggccg ctggtcacta acctgggcgt
     4981 ctatatggcg gggatcgcgc tgggggcagt cataactgcg ctgatggtgg tgttcctgcg
     5041 tctgatgatg ttccgtaaag gcaaattgtt aatcgatagc ctgtaagaag gaaaatccag
     5101 atgacattac tcgcttcgct gcgcgactgg cttaaggcgc aacaactgga tgcagtgctt
     5161 ctctcctcac ggcagaacaa acagccgcat ctggggatct ccaccggatc aggttatgtg
     5221 gtgattagcc gtgaaagtgc gcacattctg gtggattcgc gctattacgt tgaggtggaa
     5281 gcccgtgcgc aaggctacca gctgcatttg cttgacgcga cgaacacgct taccactatc
     5341 gtcaatcaaa tcattgccga tgaacagttg caaacgctcg gttttgaggg ccagcaggtg
     5401 agttgggaaa ccgcgcaccg ctggcagtct gaactcaatg cgaaactggt tagcgccacg
     5461 ccggatgtgc tgcggcaaat caaaacgcca gaggaggtgg agaaaatccg cctcgcctgt
     5521 gggattgctg atcgcggtgc agagcatatt cgccgcttta ttcaggcggg gatgagcgag
     5581 cgcgagatag ccgctgaact ggagtggttt atgcgccagc agggcgcaga aaaagcctct
     5641 tttgacacca ttgtcgccag tggctggcgt ggggcgctgc cgcacggcaa agccagcgac
     5701 aagattgttg cagcgggcga gtttgtcact ctcgatttcg gtgcgctgta tcagggctac
     5761 tgctctgata tgacgcgcac cttgctggtg aatggcgaag gggtgagcgc cgaatctcac
     5821 ccgctgttta acgtctatca aattgtcctg caggcacagc tcgcagcaat ctccgcgatt
     5881 cgccccggcg tgcgctgcca gcaggttgac gatgccgcgc gccgggtcat tacagaagca
     5941 ggttatggcg actatttcgg tcataacacc ggtcacgcta tcggcattga agttcatgaa
     6001 gatccgcgtt tttcaccgcg ggacaccacg acgctacagc caggcatgtt actgaccgtg
     6061 gagccgggga tttatttgcc agggcaaggg ggcgtgcgca tcgaagatgt tgtgctggtt
     6121 accccgcaag gcgcagaagt gctctacgcc atgccgaaaa cagtgttgct cacgggagag
     6181 gcataatgga tttatcgcta ttaaaagcgt tgagcgaggc agatgcgatc gcctcctcgg
     6241 aacaggaagt gcggcagatc ctgctggaag aagcggatcg cctgcaaaaa gaagtgcgat
     6301 ttgatggtct gggatcggtg ctgatccgcc tgaatgaatc gacaggtccg aaggtgatga
     6361 tctgtgcgca tatggacgaa gtgggattta tggtgcgcag catctcccgc gaaggggcga
     6421 ttgatgtgct gccggttggc aacgtacgca tggctgcccg ccagctgcag ccggtgcgca
     6481 tcaccacccg tgaagagtgc aaaattccag gcctgcttga cggcgaccgg caggggaatg
     6541 acgtcagcgc catgcgcgtg gacattggtg cgcgctccta tgacgaagtg atgcaggcgg
     6601 gaattcgtcc cggcgatcgc gtcacgtttg ataccacttt tcaggttctc cctcaccagc
     6661 gagtgatggg gaaagccttt gatgaccgcc tcggttgcta tctgctggtg acgttactgc
     6721 gcgaactgca cgacgccgaa ctgcctgcgg aagtgtggct ggtggcaagt tccagcgaag
     6781 aggtgggatt acgcggcggg caaactgcca cccgcgcggt gtcgccggac gtcgccattg
     6841 tgcttgatac cgcctgctgg gcgaaaaact ttgattatgg cgcggctaac catcgccaga
     6901 ttggtaacgg gccgatgctg gtgttaagcg acaagtcgct gattgcgccg ccaaaactta
     6961 ccgcctgggt cgaaaccgtg gcggcagaaa ttggcgtgcc gttgcaggca gatatgttca
     7021 gcaacggcgg cacggacggc ggggcggtgc acttaaccgg caccggcgtg cccacagtgg
     7081 tgatggggcc agcaacccgc catggacatt gcgccgcatc gattgccgat tgccgcgaca
     7141 ttttgcagat gcagcaactt ttatctgccc ttattcaacg tcttacgcgt gagacggttg
     7201 ttcaactgac ggatttcaga tgatctcctg attaacccgg agcggttatg ttaacgattc
     7261 aatttctctg tcctttgcct aacggtctac atgctcgtcc ggcgtgggaa cttaaagaac
     7321 agtgcagcca gtggcaaagc gaaatcactt ttattaacca tcgccagaac gcaaaggcag
     7381 atgcgaaaag ctcgctggcg ctgattggca ccggccccct atttaatgac agttgcagcc
     7441 tgaacattag cggcagcgat gaagagcagg cgcggcgcgt gctggaagag tacatccagg
     7501 tgcgctttat cgacagcgac agcgttcagc ctacgcaggc agaactgacg gcgcatccgc
     7561 tgccgcgttc attaagccgc cttaacccgg atttactgta cggcaatgtg ctggcaagcg
     7621 gcgtcggcgt gggtacgctg accctgttac agagcgacag cctggacagt taccggatga
     7681 tcccggccag tgcgcaagat tccacccggc tggagcacag cctggcaacg cttgccgagc
     7741 aactgaacca gcaattgcgt gagcgtgacg gcgaaagcaa aactatcctc agcgcccatt
     7801 tgtcgctgat tcaggatgat gaatttgcag gcaatatccg tcgcctgatg acagaacaac
     7861 atcaggggct gggtgtagcg atcatcagca atatggagca ggtttgcgcc aaactttctg
     7921 cttctgccag tgattatctg cgcgaacgtg ttagcgacat tcgcgatatt agcgaacagt
     7981 tgctgcatat cacctggccg gaactgaagc cgcgcaacaa tctgatgctg gaaaaaccga
     8041 ctattctggt ggccgaggat ttaaccccaa gccagttttt gagcctcgat ttaaaaaatc
     8101 ttgcaggcat gattctggag aaaaccgggc gcacctcgca taccctgatt ctggcccgcg
     8161 cctcggcgat cccggttttg agcggcctgc cgctggatgc gatcgcgcgc tatgccgggc
     8221 aacctgcagt acttgacgcc cagtgcggcg tgctggcgat taacccgaat gacgcggtga
     8281 gcggttatta tcaggtcgcg cagacgctgg cggataaacg ccaaaaacaa caggcgcagg
     8341 ctgccgcgca gctggcttat tcccgtgata acaagcgtat tgatatcgcg gctaatattg
     8401 gcaccgctct ggaagcgcct ggcgcgtttg ccaacggcgc ggaaggcgtc gggctgttcc
     8461 gtaccgaaat gctctatatg gatcgcgaca gcgcgccgga cgagcaggag caatttgaag
     8521 cctaccagca ggtgctactg gcggcgggcg acaagccgat tatcttccgc acaatggaca
     8581 tcggaggcga taaaagcatt ccttatctga atattcccca ggaagagaac ccgttcctcg
     8641 gctatcgcgc ggtacgtatt tacccgaaat ttgctggcct gttccgcact caactgcggg
     8701 ccattttgcg cgccgccagt tacggcaacg cccagttgat gatcccgatg gtccacagcc
     8761 tcgatcagat cttatgggtg aagggcgaga tccaaaaagc gatcgttgag cttaagcgcg
     8821 atggcctgcg tcatgcagag acgattacgc tgggtatcat ggtggaagtg ccgtcggtgt
     8881 gctacatcat cgatcacttc tgcgatgagg tcgatttctt cagtatcggc tccaacgata
     8941 tgacccagta tctgtatgcg gtcgatcgta ataacccgcg cgtatcgccg ctatataacc
     9001 cgattacgcc atcgttcctg cgcatgttgc agcaaatagt taccactgcg catcagcggg
     9061 gcaaatgggt aggcatttgc ggtgaactgg gcggtgaaag ccgttatctg ccgctactgc
     9121 ttgggctggg cctggatgag ctgagtatga gtagcccgcg tattccggcg gtgaaaagcc
     9181 agcttcgtca actggatagc gaggcgtgtc gggaactggc gcgtcaggca tgtgaatgcc
     9241 gcagtgcgca ggaaattgaa gcgttactca ccgcctttac gccggaagaa gacgttcgcc
     9301 cactgctggc gctggagaat atctttgttg atcaggattt tagcaataaa gagcaggcga
     9361 tccagttcct gtgcggcaac ctcggcgtta acgggcgcac tgaacatccg tttgagctgg
     9421 aagaagatgt ctggcagcgg gaagagattg ttaccaccgg cgttggtttt ggcgtagcga
     9481 tcccgcacac caaatctcag tggatccgtc attccagtat cagcattgcc cggctggcga
     9541 aaccgattga ctggcagtca gaaatgggcg aagtcgaact ggtgatcatg ctgacgctgg
     9601 gtgctaacga agggatgaat catgtgaaag tcttctcgca gctggcgcgt aaactggtga
     9661 ataaaaactt ccgccagtcg ttgtttgccg cgcaagatgc acaaagtatc ctgacgctgc
     9721 tggaaacaga attaaccttc tgacgttagc cctgaaaacg ggcgctgtac tctcccggcg
     9781 tcagaccaaa ctgacgccgg aaaacgcgac aaaaatagtc gctatccgga aaaccgcaac
     9841 gctgtgccac ttcatgaatt gacagatgat atttctgcaa aatcatcctc gccttcgcca
     9901 ttcgcaccca acgcacatac tcgataaaac gcatcgttcc atgctgagca aacagttttg
     9961 acagatgatt gggcgtgata ttaaaaaact gcgccacgct ctcgcgggtg agcggctggg
    10021 cataattatc ctgaacccag ttacaaatgc tgtgatagag aaaatcgccg cgcggttgcg
    10081 aattacccgg cggcatattc accacgttgc ggcaaagatg cagcaaactt aacaccagtg
    10141 gctgaatgat attctgctcc agcggcgaac ggctaagatg agtgagggcg gtaagcatcg
    10201 cttcaccttc accgcgttgc aggctgggaa gctggatctg gcgagcagga tgcaacaacg
    10261 acgtggtgcg gctatcgtaa aacgataacc caagccaggt cggtgcgaac accaggctta
    10321 acagcatcac cggtttgttg ttgatcggta aattagccgc gcgtgccgga ataaacagca
    10381 tttctccttc caccattcgc tgctgctttg cttccagcag gttgccgtat tcgccgcgca
    10441 gcacgatatc cagacgggga aaatcaatac ataaactgcc caccggtaac gaggcaggct
    10501 ggctggcaaa ccagacgcgc cccagtagtt gcgggttaag caccaggccg ctgaacagat
    10561 cggcgaaaaa ttgttgatcg gcgggcaagc ctggtgcttt catgaaagtt cccgattaaa
    10621 gatgcattaa ctggcgaaat tctttcactt tgctgcggct gaccggcact tcaaaatcca
    10681 gatctttcag tcgcagaatg taggtgttat taaaccacgg ttcgatttcg cgtattttgt
    10741 tcagattgac acaaaatgag cgatggcagc ggaaaaaatg cgacggcggc agtttgctgc
    10801 aaaattcggt aatgttcatc ggcattacgt aggattcacg ccgcgtatag acaaaggtca
    10861 ttttctcgtg cgcttcggcg taatagatat cgttaattgg cgtgacgatt attcgctcat
    10921 ctttcaccag attaatggtg tcattctcac gcgttaccgt cgcggcaggc gtactgctgg
    10981 tctgctgttg ttgccaggcc gcttccagtt tttgcagcat cccggtaata cgtgactcct
    11041 gatacggttt gagaatgtag tcaaacgcct ccagttcaaa cgcttctacc gcatgttctt
    11101 tccacgcggt gatgaacaca ataaacggtt tatgggcgaa ctggctgatg ttttgcgcca
    11161 gcaacacgcc atccagcgac ggaatattga tatccagaaa aatggcgtcg acgcggttat
    11221 gctgcaaaaa cttcaacacg tccagaccgt cgtcaaaggt gccgacaatc tccatctggc
    11281 tgtgctcttt aattagccag ctcagttcct gttgtgccag gaattcgtct tcaacaatga
    11341 tgactttcac aatatcactc cggctcaaag caataacgta gcctgtgagg cgactggggt
    11401 gcgttggtta ggaatgtaaa acgcaatttc cgtccccggc tccaggcggc ggatatgcag
    11461 cccctcgcca tacaataact tcacgcgatg atggacattc agcaggccaa ttttattgcc
    11521 cggcatttca ttcgcttcga cccgctcaat cacctttgga tcgatgccgt gcccggtatc
    11581 tcgcaccgca atgcgtaccc gatttccgca ctctgcaacg ctgatggtga caacgccttt
    11641 acctttgcaa ggctgaatac cgtggacaat ggcgttctcc accaacggct ggatcagcag
    11701 gctgggaatg cagcaattca cctcttcatc aatatcatag ataaccgtca gcttgtcacc
    11761 aaagcgggcc tgctcaatgg caatatagtc tttaatttga tacagctctt ttttgatatc
    11821 gatttgctca tcgtctttta attcaatgtt atagcgcaga taacgcgaca gattaaagat
    11881 caactggcga gcggtatccg gattcagacg gattgacgat gaaatagcgt tcagagcgtt
    11941 aaacagaaaa tggggattaa ttttgctttg cagggcgcga agctctgcct tatttgccat
    12001 ttcacgtagc tgctcggcgc gtgaaacctc cagttgcgtg gagatgattt gcgacagacc
    12061 gaccgccatt tcctgtaatg acgaggtgat ctgatgcgcg tggcagtagt aaattttcag
    12121 cgttccggtc acgacccctt tctcccacaa tgggatcacc agcatggaat gaatctctgg
    12181 tgtgcggtgg gcttcatcat tgtttttaat gatgattttt ccgtaattca tcgcctgacg
    12241 ggtagtcggg ctaatgaagt catcgccatt ctgatagtta tgttcaccca cgccaacata
    12301 ggccagcaca tgatcggtat tagtaatcgc caccgcatcg gcgtgaatgt catcgcggat
    12361 aatttcgcag accttgcgta atgactcgct attgacatgg cgaaacagcg gtagcgtttt
    12421 gttggcgata tccagcgcca gcttggcctg ccgcgcggcg ctggcctctt tttcgccctc
    12481 aacgctttgc accagaagca caataaagcc gatacagacg ctaccgagga tcattggaat
    12541 gccgatttta gagacgatat cgatccccaa cgcggtagtt ggtgcccaga caattaccag
    12601 aatcatggtc aacgtctcac acaacatgcc gccgagaata ccgacgcgcc agcgctgtgc
    12661 tttggggatt ttcaggttga tccagcccga tatacaaccc gccagaatgc tggtgataaa
    12721 gcaggggatc gccgtcacgc cgccgatatc aattaaatac cggtgaatac ccgcaatcac
    12781 gccggtaatg atgcctaccc acgggccaaa cagaatcccg ccggacatca ccgcgataat
    12841 acgtacgttc accagcgagc cttctacggg aacgccggac caggtgctga acagggcgaa
    12901 cagcgagaaa atggcggtaa cggcgagcaa ttcttttggg gagtgcgccg acttgtgcaa
    12961 cagttcgcga aacaggcgga tacggatgag aaagaacagg cagataagca ttaacgctgc
    13021 ccgatcgaag accgccagca gcatgttgaa tatttcgtgc acgggtgact ctgaataagg
    13081 attaaacctc tatgataggt aacctgaagg ctgatgacca gcaggccgtt tttgaggaaa
    13141 aaccacaatt ttaaaagggg attgatagat aaatggtgta ttagcaatgc atctcttga
//
LOCUS       DAOFQD010000127        13105 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.127, whole genome shotgun sequence.
ACCESSION   DAOFQD010000127 DAOFQD010000000
VERSION     DAOFQD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.127"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(18..2774)
                     /gene="barA"
                     /locus_tag="QBF46_003825"
     CDS             complement(18..2774)
                     /gene="barA"
                     /locus_tag="QBF46_003825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDQ6675952.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMGDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            2831..4132
                     /gene="rlmD"
                     /locus_tag="QBF46_003826"
     CDS             2831..4132
                     /gene="rlmD"
                     /locus_tag="QBF46_003826"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDQ6675953.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            4180..6414
                     /gene="relA"
                     /locus_tag="QBF46_003827"
     CDS             4180..6414
                     /gene="relA"
                     /locus_tag="QBF46_003827"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDQ6675954.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAELKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            6492..6740
                     /gene="mazE"
                     /locus_tag="QBF46_003828"
     CDS             6492..6740
                     /gene="mazE"
                     /locus_tag="QBF46_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDQ6675955.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            6740..7075
                     /gene="mazF"
                     /locus_tag="QBF46_003829"
     CDS             6740..7075
                     /gene="mazF"
                     /locus_tag="QBF46_003829"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDQ6675956.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            7146..7937
                     /gene="mazG"
                     /locus_tag="QBF46_003830"
     CDS             7146..7937
                     /gene="mazG"
                     /locus_tag="QBF46_003830"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDQ6675957.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            8165..9802
                     /gene="pyrG"
                     /locus_tag="QBF46_003831"
     CDS             8165..9802
                     /gene="pyrG"
                     /locus_tag="QBF46_003831"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDQ6675958.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            9890..11188
                     /gene="eno"
                     /locus_tag="QBF46_003832"
     CDS             9890..11188
                     /gene="eno"
                     /locus_tag="QBF46_003832"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDQ6675959.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(11248..12120)
                     /locus_tag="QBF46_003833"
     CDS             complement(11248..12120)
                     /locus_tag="QBF46_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDQ6675960.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSDGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(12134..12274)
                     /locus_tag="QBF46_003834"
     CDS             complement(12134..12274)
                     /locus_tag="QBF46_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675961.1"
                     /translation="MSEENKENRFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            12413..13084
                     /gene="queE"
                     /locus_tag="QBF46_003835"
     CDS             12413..13084
                     /gene="queE"
                     /locus_tag="QBF46_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDQ6675962.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT         3394 a         3102 c         3398 g         3211 t
ORIGIN      
        1 actcgacaag acatccatta cccgagaatt ttgctggctt cgcgcgcgac attatccatc
       61 tcgtccaaca gttccagcag ctccggctcc aaatcttctt ctttagtacc gctacgtagc
      121 tgttgttcga tcagttggca gagattcttc atacgcggca caccgctata gccgcaactg
      181 ccatgcagtt tatgaatcaa atcaaccagg ccttccgggt tttctccaac cagctgttcc
      241 tcaactttgt tgcgaacttc aggcaggaaa tcgagtaaca tttgcagcat atcgcgcgct
      301 aaatcggttt ttcctgctgc ctggcgtagt gccagttgcc agtcgagggt cgcattcggg
      361 ttcaccacaa tttcgttgac ttcgggcgtc acgacgcgag aggaaatacc gctgccaggc
      421 ttgtagcgca acaacaaatt atgcaatcgc tcttcttcaa tcggtttcgc cagataatcg
      481 cccatccctg cgccaagcag cttctctttt tgcccggcca ttgcatgcgc cgttaccgcg
      541 ataaccggcg tttgttgctg atgcgggagt tggtggatga gttcgcaggc ccgaatgcca
      601 tccatgtccg gcatttgaat atccattaag atcaaatcga acggcatctg tttcgcccgt
      661 tcaaccgcct gatgcccgct atcgcaaagt tccacatgtt gcaccatatc ttccagcaat
      721 gcgccgataa gtttcaggtt agcggggttg tcatcaaccg ccatgactgt cattgccagc
      781 ttactttcat cggttacagg caaaagcgtg ttttgtttgt gatgacaaaa ttccgtcagg
      841 gcaggcaaca ggcgtgtagg tgttaatggt ttcagcagac acgcgccgat accatcttgc
      901 ttgagttttt cagcattgac ttgtgcatgg caaggaagtg ccagcatcag gaaatcggtc
      961 atcgataccg ctttcgctaa tcgctcatgt tgcattgtta gcggctcgcg gaatgtcacc
     1021 gcgatgccta acaacatcat gtcgtaatgc gcgggaggca gcgcggagaa cgttgggcta
     1081 taaaccactt ccagcggcgt ttcacttaaa atatccagcg tgcattgcgc tgctgcggag
     1141 tttggttcga cataggccag gcgcttacct gcgaggcact gggtggatgg cccttcgata
     1201 ataatgttcg ggttcagatc gagattaatg tggaaccaga aagttgaacc gcgattcggc
     1261 tggctatgga acgaaatatc gccgcccatt tcattaacca gtttttgtgt aatcaccagc
     1321 cccagcccgg tgccaccatg acggcgggaa atactggcat cagcctgtcg gaaggcctgg
     1381 aataagcgcg attgatcgcg ttcaggaata ccaatgccgg tatcccgaat ctgcacttca
     1441 atctgcactt tggtattact cagcgcacgt ttttctacca gaatatcaat gttgccattc
     1501 tcggtgaatt taattgcatt ccccaccagg ttagtgatga tttgctgtaa tcgcagcggg
     1561 tcgccgatca cgttatcagg cacgtcgctt ttaatattga gcgtcagttc caacccttta
     1621 tcgtgagaag aatgtgccag cagagtaacg acttcatcca gcgtgctgcg tagtgggaat
     1681 ggaatacttt ccagaatcag cttacctgct tccagtttcg agaagtcgag aacatcatta
     1741 ataattgcca gcaaattatt tgccgaacgt tcaatcgtat tcaggtgatc gcgctgcgtt
     1801 ggtgttaatt ctgttttcag cgtcaggcgg gtaaagccaa taacaccatt cagtggtgta
     1861 cgcagctcgt gtgacatatt tgccagaaac tcggatttaa tacgcgccgc ttcctgggcg
     1921 cgctttttcg ccagatccaa ctcaacgttc tgaatttcca tctgctccag cgtttcacgc
     1981 agatcggacg tcgcctggtc gatattgtgc tgcatctctt cgtgataagc agccagcgac
     2041 attgccatcg agttgatacc gtttttcagc atatccagct cgccgagcat aaatccttcc
     2101 acccggctgt cgagttgccc gcgacggatg cggtcgacgg tattcaccat gttgcgaatc
     2161 ggaccggtta catcgcgcat taagcgccag ccaaaaataa gcgcaatacc gatacaaaac
     2221 agcatcatca cgctggaaat aaagatctct ttatattgct gcaagcgaac cgatttaaga
     2281 tccagctcca gcgcaatata tcccaacata ttttgactat ttttggcatc gctactgggc
     2341 gattcgtcgg gggagtaact ctcagaaata atcggcgtgc ggaggatcat aatatcgccg
     2401 tcacgagtga cagtgagctg gcgaggaaac ggcacgttgc tgccgagctg cattgatgag
     2461 ggatcaagat gaaaattgga ggtgacaaag agtcggttat tttcatcata aaccgaaatc
     2521 gcgcgaacaa tatcggaatg gcgacgatgc agtacgctta ttaactgacc gatagattcg
     2581 cgattttgca ggctcatgcc atattcagta gaaactgcaa gcggctcaat aatgctggca
     2641 ccggcatctt ccagttgacg ctgcaagtcg ttatagcgat gcacgacgaa aaagatactc
     2701 agcaataaac caataaggac ggtcggtgcc aggatcagaa tcatcatgcg tgcgcgcagg
     2761 ctgtagttgg tcatggagtt ccgttatggg acaattaagg tcacactgtt aaattgagaa
     2821 agtctcgcca atggcgcaat tctactctgc aaaacgacgc acgacgacgc gtcagatcat
     2881 aaccgtttca gtcaacgacc tcgactcttt tggtcagggc gtggcgcgac ataacggcaa
     2941 agcgttattt attcctggat tactgccaca ggaaaacgcg gaagttactg ttactgaaga
     3001 taaaaaacag tacgcccgcg ctaaagtcgt gcgccggtta agcgatagcc cggaacgcga
     3061 aacgccacgc tgccctcatt ttggcgtatg cggtggctgt cagcaacaac acgccagcgt
     3121 ggatttacag cagcgaagca aaagtgcggc actcgcccga ttaatgaaac acgaagtctc
     3181 tgaagtgatc gccgatgttc cctggggcta tcgccgtcgc gcgcgtttaa gtttgaacta
     3241 cttaccgaaa acacagcaac ttcagatggg ctttcgcaaa gcgggctcca gtgacattgt
     3301 cgacgttaaa caatgcccca ttttagtgcc ccaacttgaa gcattgctgc ccaaagtcag
     3361 ggcatgcctg ggcagcttac aagctatacg ccatcttggt catgttgaac tggtacaggc
     3421 aaccagcggc acgctgatga ttttgcgcca taccgcaccg ctaagttcgg cagatcgcga
     3481 aaaactggaa cgcttttcgc attctgaagg cctggatctg tatctcgccc ccgatagtga
     3541 gatactcgaa accgtctctg gtgagatgcc ctggtatgac tcaaacgggt tgcgcttaac
     3601 ttttagcccg cgcgatttta ttcaggtcaa tgcgggtgtg aaccaaaaaa tggtagcgcg
     3661 tgcgttggaa tggctggatg tgcaacctga agatcgcgta ctggatctgt tctgcggtat
     3721 gggcaacttt acactgccat tggcgacaca agctgccagt gtggtgggtg tagaaggtgt
     3781 tccggcgctg gtggaaaaag gccagcagaa tgcgcgtctt aatggcttac agaatgtgac
     3841 gttttatcat gaaaatcttg aagaagatgt cacaaagcag ccgtgggcga aaaacggctt
     3901 cgataaagtg ttgctggacc ccgcgcgagc aggtgccgca ggtgttatgc agcaaattat
     3961 aaaactggaa cctattcgta tagtttatgt atcctgtaat cctgcaacgc tggctcggga
     4021 tagcgaagcg ttattaaaag caggatatac cattgcgcga ctggcgatgc tggatatgtt
     4081 cccacacacg ggacatctgg aatcgatggt acttttctcg cgcgttaaat agttacgatt
     4141 tgccgatttc ggcaggtctg gtccctaaag gagaggacga tggttgcggt aagaagtgca
     4201 catatcaata aggctggtga atttgatccg gaaaaatgga tcgcaagtct gggtattacc
     4261 agccagaagt cgtgtgagtg cttagccgaa acctgggcgt attgtctgca acagacgcag
     4321 gggcatccgg atgccagtct gttattgtgg cgtggtgttg agatggtgga gatcctctcg
     4381 acattaagta tggacattga cacgctgcgg gcggcgctgc tgttccctct ggctgatgcc
     4441 aacgtagtca gcgaagatgt gctgcgtgag agcgtcggta agtcggtcgt taaccttatt
     4501 cacggcgtgc gtgatatggc ggcgatccgc cagctgaaag cgacgcacac tgattctgtt
     4561 tcctccgaac aggtcgataa cgttcgccgg atgttattgg cgatggtcga tgattttcgc
     4621 tgcgtggtca tcaaactggc ggagcgtatt gctcatctgc gcgaagtaaa agatgcgccg
     4681 gaagatgaac gcgtactggc ggcaaaagag tgcaccaata tctacgcgcc gttggcgaac
     4741 cgtcttggga ttgggcaact gaaatgggag ctggaagatt actgcttccg ttatctccac
     4801 ccgaccgaat acaaacgcat cgcaaaactg ctgcatgaac gccgtctcga ccgcgaacac
     4861 tatatcgaag agtttgtcgg ccatctgcgc gctgagatga aagctgaagg cgttaaagct
     4921 gaagtgtatg gtcgtccgaa acacatctac agtatctggc gcaaaatgca gaaaaagaac
     4981 ctcgccttcg atgagctgtt tgatgtgcgt gcggtacgta ttgtcgccga gcgtttacag
     5041 gattgttatg ccgcactggg gatagtgcac actcactatc gccacctgcc ggatgagttt
     5101 gacgattacg tcgctaaccc gaaaccaaac ggttatcagt ctattcatac cgtggttctg
     5161 gggccaggtg gcaaaaccgt tgagatccaa atccgcacca aacagatgca tgaagatgca
     5221 gagttgggtg ttgctgcgca ctggaaatat aaagagggcg cggctgctgg cggcgcacgt
     5281 tcgggacatg aagaccggat tgcctggctg cgtaaactga ttgcatggca ggaagagatg
     5341 gctgattccg gcgaaatgct cgacgaagta cgcagccagg tctttgacga ccgagtgtac
     5401 gtctttacgc cgaaaggtga tgtcgttgat ttgcctgcgg gatcaacgcc gctggacttc
     5461 gcttaccaca tccacagtga tgtcggacac cgctgcattg gggcaaaaat tggcgggcgc
     5521 attgtgccgt tcacctacca gctgcagatg ggcgaccaga ttgaaattat cacccagaaa
     5581 cagccgaacc ccagccgtga ctggttaaac ccaaacctcg gttacgtcac aaccagccgt
     5641 gggcgttcga aaattcacgc ctggttccgt aaacaggacc gtgacaaaaa cattctggct
     5701 gggcggcaaa tccttgacga cgagctggaa catctgggga tcagcctgaa agaagcagaa
     5761 aaacatctgc tgccgcgtta caacttcaat gatgtcgacg agttgctggc ggcgattggt
     5821 ggcggggata tccgtctcaa tcagatggtg aacttcctgc aatcgcaatt taataagccg
     5881 agtgccgaag agcaggacgc cgccgagctg aagcaacttc agcaaaaaag ctacacgccg
     5941 caaaaccgca gtaaagataa cggtcgcgtg gtagtcgaag gtgttggcaa cctgatgcac
     6001 cacatcgcgc gctgctgcca gccgattcct ggagatgaga ttgtcggctt cattacccag
     6061 gggcgcggta tttcagtaca ccgcgccgat tgcgaacaac tggcggaact gcgctcccat
     6121 gcgccagaac gcattgttga cgcggtatgg ggtgagagct actccgccgg atattcgctg
     6181 gtggtccgcg tggtggctaa tgatcgtagt gggttgttac gtgatatcac gaccattctc
     6241 gccaacgaga aggtgaacgt gcttggcgtt gccagccgta gcgacaccaa acagcaactg
     6301 gcgaccatcg acatgaccat tgagatttac aacctgcaag tgctggggcg cgtgctgggt
     6361 aaactcaacc aggtgccgga tgttatcgac gcgcgtcggt tgcacgggag ttaggccgaa
     6421 atttgctcgt atctacaatg tagattgata tatactgtat ctacatatga tagcggtttg
     6481 aggaaagggt tatgatccac agtagcgtaa agcgttgggg aaattcaccg gcggtgcgga
     6541 tcccggctac gttaatgcag gcgctcaatc tgaatattga tgatgaagtg aagattgacc
     6601 tggtggatgg caaattaatt attgagccag tgcgtaaaga gcccgtattt acgcttgctg
     6661 aactggtcaa cgacatcacg ccggaaaacc tccacgagaa tatcgactgg ggagagccga
     6721 aagataagga agtctggtaa tggtaagccg atacgtaccc gatatgggcg atctgatttg
     6781 ggttgatttt gacccgacaa aaggtagcga gcaagccgga catcgtccgg ctgttgtcct
     6841 gagtccgttc atgtacaaca acaaaacagg tatgtgtctg tgtgttcctt gtacaacgca
     6901 atcaaaagga tatccgttcg aagttgtttt atccggtcag gaacgtgatg gcgtagcgtt
     6961 agctgatcaa gtaaaaagta tcgcctggcg ggcaagagga gcaacgaaga aaggaacggt
     7021 tgccccagag gaattacaac tcattaaagc caaaattaac gtactgattg ggtagtgtta
     7081 ctaactcact tctattctgg tcacaggttt ccacctgaca gacccgcaat tttcaggaca
     7141 ttctaatgaa tcaaatcgac cgtttgctca ctattatgca gcgcctgcgc gatccggaaa
     7201 acggctgccc gtgggataaa gagcagacat ttgccaccat tgcgccttac acccttgaag
     7261 aaacctacga agtgctggac gccatcgccc gtgaagattt tgacgatctg cgcggtgaac
     7321 tcggcgatct gctgttccag gtggtgtttt acgcgcaaat ggctcaggaa gaagggcgct
     7381 ttgactttaa tgatatttgc gctgcgatta gcgataaatt agagcgtcgc catccgcatg
     7441 tttttgctga cagttctgcc gaaaacagta gtgaagtgct tgcccgttgg gagcaaatca
     7501 aaaccgaaga gcgcgcgcag aaagcgcagc attcggcgct ggacgatatt cctcgtagtt
     7561 taccggcttt aatgcgtgcg caaaaaatcc agaaacgttg cgccaacgtt ggcttcgatt
     7621 ggacgacgct tggtccggta gtcgataaag tctacgaaga gatcgacgag gtgatgtacg
     7681 aagcgcggca ggctgttgtc gaccaggcta aactggagga ggaaatgggg gacctgctgt
     7741 ttgccacggt caatctggct cgtcatttag ggacaaaagc ggaaatcgca ttgcaaaaag
     7801 cgaacgaaaa atttgagcgt cgttttcgcg aagtggagcg tattgttgcc gcgcgtggac
     7861 tggaaatgac aggtgttgac ctcgaaacaa tggaagaagt ctggcaacag gtaaaacggc
     7921 aggaaattga tctctaaggg aattacgcgg tcaagcgcca tttgtgtcat tttttaaatg
     7981 acaagcgctt gatttgcgtc aaaaacattt accccaaagg ggctattttc tcactcctga
     8041 tttcaatagt gcgctggcga agaggaggga taatgaaagt ttgtggcaca ggtcatgttc
     8101 gggtatactg ctttcccgtc ctggttattc catcgtcttt tcaacctaac ttctcaggtt
     8161 cagcatgaca acgaactata tttttgtgac cggcggggtc gtatcctctc tgggtaaagg
     8221 cattgccgca gcctccctcg cagccattct tgaagcccgt ggcctcaatg tgaccatcat
     8281 gaaactggat ccttacatca acgtcgatcc aggtactatg agcccaatcc aacacgggga
     8341 agtgttcgtt actgaagacg gcgctgaaac cgacctggac ctggggcact acgagcgttt
     8401 cattcgtacc aaaatgagcc gccgcaacaa cttcaccacg ggtcgtatct actctgacgt
     8461 tctgcgtaaa gaacgccgcg gtgactacct cggcgcaacc gtgcaggtta ttccgcacat
     8521 cactaacgca atcaaagagc gcgtgctgga aggtggcgaa ggtcatgacg tagtactggt
     8581 agaaatcggc ggtacagtag gtgatatcga atccttgccg ttcctcgaag cgattcgcca
     8641 gatggctgtt gaaattggcc gtgagcacac tctgtttatg cacctgacgc tggtgccgta
     8701 catggcagcg tctggtgaag tcaaaaccaa accgactcag cactctgtaa aagagctgct
     8761 ctccatcggt atccagcctg acatcctgat ttgtcgttca gatcgcgctg ttccggcgaa
     8821 cgaacgtgcg aagattgcat tgttctgtaa tgttccggaa aaagcggtta tttctctgaa
     8881 agacgtcgat tccatctata aaattccggg cctgttgaaa tctcaggggc tggacgatta
     8941 tatttgtaaa cgattcagct taaactgccc ggaagcgaat ctgtccgaat gggaacaggt
     9001 tatcttcgaa gaagcgaacc cggtaagtga agtcaccatc ggtatggtcg gcaagtacat
     9061 tgaactgccg gatgcttata aatcagtgat cgaagcactg aaacacggtg ggctgaagaa
     9121 tcgtgtcagc gtcaacatca aactgatcga ttcacaagat gttgaaacgc gcggcgttga
     9181 aatccttaaa ggtctggacg caatcctcgt acctggcggt ttcggctatc gtggcgtaga
     9241 aggcatgatt acgaccgcgc gttttgcgcg tgagaacaat attccttatc tgggcatttg
     9301 cctgggtatg caggtggcgt taattgatta cgctcgccat gttgccaaca tggagaacgc
     9361 caactctacg gaatttgtgc cagactgtaa gtacccggtt gtggcgctga ttaccgagtg
     9421 gcgcgatgaa aacggcaacg ttgaagttcg tagcgagaag agcgatctcg gcggtaccat
     9481 gcgtctcggc gcacagcagt gccagttggt tgacgatagc ctggttcgcc agctgtacaa
     9541 tgcgccgaca attgttgagc gtcatcgtca ccgttacgaa gtcaacaaca tgctgttgaa
     9601 acagattgaa gatgcaggtc tgcgcgttgc aggccgttcc ggggatgatc agttggtcga
     9661 gatcatcgaa gttccgaatc acccgtggtt cgtggcttgt cagttccatc cggagtttac
     9721 ttctactcca cgtgatggtc acccgctgtt tgctggcttt gtaaaagccg ccagcgagtt
     9781 ccagaagcgt caggcgaagt aagtaaaaaa gttagaacgg caacgcgtac cctgggtacg
     9841 cgttgtttgt ctggagtttc agtttaacta gtgacttgag gaaaacctaa tgtccaaaat
     9901 cgtaaaaatc atcggtcgtg aaatcatcga ctcccgtggt aacccgactg ttgaagccga
     9961 agtacatctg gagggtggtt tcgtcggtat ggcagctgct ccgtcaggtg cttctactgg
    10021 ttcccgtgaa gctctggaac tgcgcgatgg cgacaaatcc cgtttcctgg gtaaaggcgt
    10081 aaccaaagct gttgctgcgg taaacggccc gatcgctcag gcgctgattg gcaaagatgc
    10141 taaagatcag gctggcattg acaagatcat gatcgacctg gacggcaccg aaaacaaatc
    10201 caaattcggc gcgaacgcaa tcctggctgt atctctggct aacgccaaag ctgctgcagc
    10261 tgctaaaggt atgccgctgt acgagcacat cgctgaactg aacggtactc cgggcaaata
    10321 ctctatgccg gttccgatga tgaacatcat caacggtggt gagcacgctg acaacaacgt
    10381 tgatatccag gaattcatga ttcagccggt tggcgcgaaa actgtgaaag aagccatccg
    10441 catgggttct gaagttttcc atcacctggc aaaagttctg aaagcgaaag gcatgaacac
    10501 tgctgttggt gacgaaggtg gctatgcgcc gaacctgggt tccaacgctg aagctctggc
    10561 tgttatcgct gaagctgtta aagctgctgg ttatgaactg ggcaaagaca tcactctggc
    10621 gatggactgc gcagcttctg aattctacaa agacggtaaa tacgttctgg ctggcgaagg
    10681 caacaaagct ttcacctctg aagaattcac tcacttcctg gaagaactga ccaaacagta
    10741 tccgatcgta tctatcgaag acggtctgga cgaatctgac tgggacggtt tcgcatacca
    10801 gaccaaagtt ctgggcgaca aaatccagct ggttggtgac gacctgttcg taaccaacac
    10861 caagatcctg aaagaaggta tcgaaaaagg tatcgctaac tccatcctga tcaaattcaa
    10921 ccagatcggt tctctgaccg aaactctggc tgcaatcaag atggcgaaag atgctggcta
    10981 cactgcagtt atctctcacc gttctggcga aactgaagac gctaccatcg ctgacctggc
    11041 tgttggtact gctgcaggcc agatcaaaac tggttctatg agccgttctg accgtgttgc
    11101 taaatacaac cagctgattc gtatcgaaga agctctgggc gaaaaagcac cgtacaacgg
    11161 tcgtaaagag atcaaaggcc aggcataaga ctgactttat ctgatttaaa aatgccagcc
    11221 tccgggctgg cattttttat ggttgagtta ccagcggccg gatgcgccgc cacctccaga
    11281 agaaccgccc ccgccgctaa atccgccgcc acttgagcct ccatcggaac cgctgttgtc
    11341 tgaatgataa cttttacctg atgctctttt ctgaagtaca cataaacagg taaagaccat
    11401 aaatatggtg aaagtaaaaa agaaggaaaa tacagcaatg gaaaaaattt tatcggagaa
    11461 caggaataaa tctaaaagat agattgctga aatacagact ccacttttac atgcgcgaca
    11521 aaaattactg ccttgatgaa aaaaccagaa agggaagaac atcactgcaa atacccaaaa
    11581 aatagcaaag taataatgat cactggaaga cgctgattca ctttctgaag gattagtcgg
    11641 atattggtgt tgtgaagtga gttggttatt caaagcgttt atagctagct ctaatccctg
    11701 agcaaatttt tgttgtttaa atgcgggtat catgttgcta cggatgatat ctccagccag
    11761 agcatcggtt accttttctt ccagcccata acctacctgg atgcggacag tacgatctga
    11821 ccaggcaaca atgatcagta ttccatcatt acgtttggca tctcctaaac gccaattgtc
    11881 aaaaactctt gtagcatatt gttcaattgt ctcgtctttg gttgtgggaa caattaatac
    11941 agcaatttgc acttgggtat tattgaaaat agattctaac ttgtgcgtca gttctttctt
    12001 ttcttgtgag tttaagctac tcgtaagatc cgtaacttgt tgctgtaatt gtgggacaat
    12061 agttggttgg gctgcaacaa aggaacacac gaaagtgaac attagtatga aatagcgcat
    12121 tataaatttc tcattaaatc tggataaata aattgacggc ggcatcggta attttagatt
    12181 cgttatcatt aaaaactaac acagaaaaaa taaatataat tttggtgaat gttttgacat
    12241 gattaaatct attttcttta ttttcttctg acataatacg ttactccctt taattattaa
    12301 cgatatgttt cgcatttttc tttaaatctg cctgtaacag aatgttaata agacatgatt
    12361 gttgtggcag gatctgcaat aattaacaat tatccacttc acagagaatg ctatgcagta
    12421 cccgattaac gagatgttcc agaccctgca aggtgagggt tactttaccg gcgtccccgc
    12481 catttttatt cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca ccaaacacac
    12541 ctgggaaaag cttgaggatc gggaagtctc ccttttcagc attctggcga agaccaaaga
    12601 gagtgataag tggggggcgg cgagcagtga agacttgttg gctgtcatta gtcgccaggg
    12661 atacaccgcg cggcatgtgg tgattacggg tggtgagcct tgcattcatg atttgctacc
    12721 actgactgat ctgctcgaaa agaacggttt tagctgccag atcgaaacca gtggtactca
    12781 tgaggtacgc tgcacaccga atacctgggt taccgtatca ccaaagctga acatgcgcgg
    12841 cggctatgaa gtgttgtcac aggcactgga gcgagccaac gaaatcaagc atccagtggg
    12901 gcgcgtacgt gatattgaag cactggatga actactggca acgctgaccg atgataaacc
    12961 gcgagtcatt gcactgcagc cgattagcca aaaggatgat gccacacgtt tgtgcattga
    13021 aacctgcatt gcgcgtaatt ggcgtttgtc gatgcaaaca cataaatatc taaatattgc
    13081 ctgattaaac atttataagc gttat
//
LOCUS       DAOFQD010000128        13099 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.128, whole genome shotgun sequence.
ACCESSION   DAOFQD010000128 DAOFQD010000000
VERSION     DAOFQD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.128"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            61..378
                     /locus_tag="QBF46_003836"
     CDS             61..378
                     /locus_tag="QBF46_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDQ6675963.1"
                     /translation="MQPVFFIKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEGIDKET
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            447..776
                     /gene="yjdP"
                     /locus_tag="QBF46_003837"
     CDS             447..776
                     /gene="yjdP"
                     /locus_tag="QBF46_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDQ6675964.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(923..1681)
                     /gene="phnP"
                     /locus_tag="QBF46_003838"
     CDS             complement(923..1681)
                     /gene="phnP"
                     /locus_tag="QBF46_003838"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDQ6675965.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTMEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNEVIHSPRV
                     ILTHIIHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(1683..2117)
                     /gene="phnO"
                     /locus_tag="QBF46_003839"
     CDS             complement(1683..2117)
                     /gene="phnO"
                     /locus_tag="QBF46_003839"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDQ6675966.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRMGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2104..2661)
                     /gene="phnN"
                     /locus_tag="QBF46_003840"
     CDS             complement(2104..2661)
                     /gene="phnN"
                     /locus_tag="QBF46_003840"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDQ6675967.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2661..3797)
                     /gene="phnM"
                     /locus_tag="QBF46_003841"
     CDS             complement(2661..3797)
                     /gene="phnM"
                     /locus_tag="QBF46_003841"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDQ6675968.1"
                     /translation="MIINNVKLVLENEVVSGSLEVQNGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDVQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(3794..4474)
                     /gene="phnL"
                     /locus_tag="QBF46_003842"
     CDS             complement(3794..4474)
                     /gene="phnL"
                     /locus_tag="QBF46_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDQ6675969.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(4585..5343)
                     /gene="phnK"
                     /locus_tag="QBF46_003843"
     CDS             complement(4585..5343)
                     /gene="phnK"
                     /locus_tag="QBF46_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDQ6675970.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQEVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5340..6185)
                     /gene="phnJ"
                     /locus_tag="QBF46_003844"
     CDS             complement(5340..6185)
                     /gene="phnJ"
                     /locus_tag="QBF46_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDQ6675971.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(6178..7242)
                     /gene="phnI"
                     /locus_tag="QBF46_003845"
     CDS             complement(6178..7242)
                     /gene="phnI"
                     /locus_tag="QBF46_003845"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDQ6675972.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPYFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7242..7826)
                     /gene="phnH"
                     /locus_tag="QBF46_003846"
     CDS             complement(7242..7826)
                     /gene="phnH"
                     /locus_tag="QBF46_003846"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDQ6675973.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(7823..8275)
                     /gene="phnG"
                     /locus_tag="QBF46_003847"
     CDS             complement(7823..8275)
                     /gene="phnG"
                     /locus_tag="QBF46_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDQ6675974.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8276..9001)
                     /gene="phnF"
                     /locus_tag="QBF46_003848"
     CDS             complement(8276..9001)
                     /gene="phnF"
                     /locus_tag="QBF46_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDQ6675975.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(9022..9801)
                     /gene="phnE"
                     /locus_tag="QBF46_003849"
     CDS             complement(9022..9801)
                     /gene="phnE"
                     /locus_tag="QBF46_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDQ6675976.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(9907..10923)
                     /gene="phnD"
                     /locus_tag="QBF46_003850"
     CDS             complement(9907..10923)
                     /gene="phnD"
                     /locus_tag="QBF46_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6675977.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(10948..11736)
                     /gene="phnC"
                     /locus_tag="QBF46_003851"
     CDS             complement(10948..11736)
                     /gene="phnC"
                     /locus_tag="QBF46_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDQ6675978.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSYIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTCVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(11869..12312)
                     /gene="yjdN"
                     /locus_tag="QBF46_003852"
     CDS             complement(11869..12312)
                     /gene="yjdN"
                     /locus_tag="QBF46_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDQ6675979.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(12570..12905)
                     /locus_tag="QBF46_003853"
     CDS             complement(12570..12905)
                     /locus_tag="QBF46_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDQ6675980.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         2601 a         3694 c         3959 g         2845 t
ORIGIN      
        1 ggtgttttat atatttatta tatatattgt acttcggcaa atactgagca caattaatca
       61 atgcaaccgg tgttttttat taaaaaacgt accggagcaa tgggcggtca tgtcgctggc
      121 accgcaggat tctgtgttgc tgtgccagcc ttttattcat ggtcaatgag tgaggggatt
      181 gataaagaaa ccaggtcgtc agagcgcact gattatccgc attacgccag tgaagttgca
      241 ctggcttttg gttcacgagt ggttggcctc gaactggcaa aaatgattgt ggatgcgtgg
      301 ctgggcgcac agtacgaagg cggtcgtcat caacaacgcg tggaggcgat tacggcaata
      361 gagcagcgga gaaattgaga ttcatccact acttgcatgg atgagtaatg actaatgtgg
      421 atagagtttc tttttgaggt tggctaatga aacgctttcc gctatttctt ttattcaccc
      481 tgctcacgct gtccaccgtt ccggcccagg ccgatattat cgacgacacc attggcaata
      541 ttcagcaggc gattaacgac gcttataacc ctgaccgtgg gcgagactat gaagattcgc
      601 gcgacgacgg ctggcagcgt gaagtgagcg acgatcggcg cagacaatat gacgaccgcc
      661 gccgccagtt tgaagatcgc cgcaggcagc tggacgatcg ccagcgccag cttgatcagg
      721 agcgtcggca gctagaagat gaagagcgga gaatggaaga tgagtatggg cgatgaggtt
      781 gggtatggag caggcatcgc ccctcacccc aatggggaga gggagaaaac cagcgcaata
      841 ttcaatatcg agcacaatcg gtcccctcgc ccctctgggg agagggttag ggtgaggggg
      901 ccagcgcgca cattccctct catcacgcca ccccaatctc catcccatca aaccccgcct
      961 caaaacccgg cggcagcgca ttctccatca gccacgcatc aaactggtgg atgatatggg
     1021 tcagaatcac ccgtggcgag tggataacct cattcagcgc aagcacggta tttaaatcac
     1081 agtgattacg cggcgcatcc gcgcgcggcg ggtgactgca atccatcacc attacctgcg
     1141 gctgattatt gcgtaaaaat ttcagcgttt tttccggcaa gcctgcggtg tcagacagcc
     1201 acgccacccg gctgtgtgcc gtttccagca gataaccgaa ggtcagtttt gagtggttga
     1261 gcggcagggg cgtgacctgt aacccctgca aatcaaacac cacaaacggt tccatcgtgt
     1321 ggctgaaatc aagcaggccc ggatgtttaa acagatcgtc gcagccctgt tcatccggcg
     1381 ggccgtaaac cgggatcgga tcgccaacgc cccagcgcag cggaaacagc ccctggacgt
     1441 gatccatatg ataatgcgtc agcaaaaact gctggaacga tccgggcgac cagcgatcgg
     1501 cgagatcgtg cagcccggcg tcgatcaggg tgattgcgtc gttaaacttc actacgccgc
     1561 tgcatggctg gcggcgatac tgcggtgagc gccgcgctcg gtcgcaggcc gcgcactcgc
     1621 agccccatgc cggaacgccc tgtgcgccgc cggtgccggt gagcgtgagg gtcagactca
     1681 tgttacagcg ccttggtgaa gcggaagtga ctctgctcgt agccttctcg cagatagaaa
     1741 cggtgcgcgt cgtggcgctt cacgttggtc gaaagctcgg tcatttcggc cccggcctgg
     1801 cgggcttctt cttctgccca tgccagtaat ttactgccga cattcagacc gcgcgcctgc
     1861 ggcatcacca ccagttcctg aatttcgccg atccagttga catgatgcag atgaaactgc
     1921 aaatgcaggc cgatcatgcc gacaacttcg ccatccagca gcgccagatg gtagcgcatg
     1981 ttcgggtcgc gcagattggc gttaaagccc atgcgaaacg cgtggtggtc aaactccgcc
     2041 tgttttagtt cacaaatcag cgcgtaaacc gcgtcggtat cgtactgcgt ggccgggcga
     2101 agctcacaag caggcatggt gtttctcctt ctgatgaatc agcgtcagca gcgtgtcgac
     2161 cgactggcgc aggctgccgt cattattgag cgtatggcaa tcctgtggag tatagcgggc
     2221 ggcgcgcgcc agccgggcgt taatttcact ggcgttttcg cggccgcggt tttccagacg
     2281 ctggcgcagg atctccggcg aaacctgtaa gcaaatgggc agcagcgccg attgatagcg
     2341 cgcccgcgcc tgcggcagat gggcgcgtga gccgttgacc agcacgtcga atccggcgtg
     2401 cagccagaga tcaatctcga cgccgacgcc ataatacaga ccgttagcgt gccagcttaa
     2461 ggccagtagg ttctgccccg cgcgggtaaa aaactcctgc tcgctcaggg cgatatggtt
     2521 ttcgcttccg gcgctggcgt cgcgcgtgat gtagcgatgc gccaccagta gctgagtttg
     2581 ttcccgcagg cggagttccg ccagcaggct atctttcccg gagccggacg gccccattaa
     2641 ccaaatcagt tttcccatca tcagaacacc cttttaccct gacgccagac gtggtcgata
     2701 tgaatgtgat tgcccttgcg atgcgccagc accaggtcgg cgcgtttgcc ctcgccaatc
     2761 accccgcgat cctggagatt aagcgcctgc gctggatttt tagtcaccag cttcaccgcc
     2821 tgcggcagcg taaagcggtt gctctcgtca tcggcgacgc gaaatgccgc atcgagcagg
     2881 ctggcggggt agtagtcgga agagaggata tccagcaggc caagctgcgc cagttcactg
     2941 gccgccacgt tgccggagtg cgagccgccg cgcacaatat tcggcgcgcc catcagcacg
     3001 ttcatgccat gcttgcgcga ggcttccgcc gcttcgaacg tggtgggaaa ttcggcgatc
     3061 acgctgccaa gctggtgtga ttcggcaacg tgggcgtgcg tggcgtcatc gtggctggcg
     3121 agggcaattt gtcgcgcgcg gcacagggcg gcgatggatt cgcgatttgg ttgcgaccag
     3181 cgtgcggcaa gcgccagctg ttcctcttcg tactgctgca tctgcacatc ggtgagggag
     3241 tatttgccct gataatattc acggtacttc tcgcggttgg cgaactggcg ctggcccggc
     3301 gagtggtcca tcagcgaaac cagcgtcacc ggctcgcgct gcaccagttt ttcaaacagc
     3361 ggcagggtgg tgtgatgcgg cagttcgcag cgcaggtgca ggcggtgctc ggcgcggttg
     3421 acgccgcgtt tctgcgtctc ttcgatggcg ttgatcatct tctccagatt ctccagccga
     3481 tcgccgccgt cgcgcacgtc gccaatcgcc accgcatcca gcacggtagt gatgccgctc
     3541 gccaccatca gcgcatcgtg gctgctcatc gccgagtggg caggccagtc aactttcggg
     3601 cgcggggtga agaatttatc cagattatcg gtatgcagct caatcagccc cggcagcagc
     3661 cagccgcctt cgccgtccat cgcctccggc aggcggctct ggctttcggc aaaggcgcgg
     3721 atttcgccgt tctgcacctc cagcgaaccg cttaccacct cgttttccag caccagctta
     3781 acgttattga taatcatgaa gaggctccca ttgggtgcag gcggtcggcg acgtcattac
     3841 gtacagcttc gtcatggaag atgcctacga tggctgcgcc acgggttttg gcttcgcgaa
     3901 tcagttccac caccgcggcg ctgtttttgg cgtccagcga ggcggtaggt tcgtcgagca
     3961 gcagaatcgg gtagtcgacg ataaagccgc gggcgatgtt gacgcgctgc tgttcgccgc
     4021 cggagaaggt tgatggcgcc aggtgccaca ggcgttccgg cacgttcagg cgggtgagca
     4081 gacgcgcggc tttagcggcg caggcttcac gcggtacgcc ggtatcgagc agcggctgca
     4141 tcaccacttc cagtgctgag atacgcggga tgacgcgcag aaactggctc acccagccga
     4201 cggtggtttt gcggatttcc accactttgc gcgctggcgc ggtgaccagg tctacccact
     4261 cgtcaccgtg tttgatctgg atttgacctt cgtcgggtag atagttggcg tacagcgagc
     4321 gtagcagagt tgatttgccg ctgccggaat ggccgtggag caccacgcat tcgcccgcgt
     4381 tgacggtgag cgaggcgcga ttgaggacgg gcaggcgcac gccgttttgc tggtgcagga
     4441 tgaaggtttt actgacgttt tgtacgttaa tcatttttcg cctcgtggtt cgggtgtcgg
     4501 atgcgacgct gacgcgtctt atccgaccta cggggagcgc atttgtaggc cggataaggc
     4561 gtttacgccg catccggcac cggctcaatt ctgcaaaacc gatgacacca gcagctgtgt
     4621 atacggatga tgcgggtcgt cgagcacgcg gtcggttaac ccactctcca cctcttgccc
     4681 ctgcttcatc accagcaaac ggtccgccag caggcgggcg acgcctaaat catgggtgac
     4741 aatcaccacc gcgaggttca gctccaccac caggccgcgc agcaggtcga gcaggcgggc
     4801 ctgcaccgac acatccagcc cgccggtcgg ttcatccata aacaccagct tcggatgcgt
     4861 caccaggttg cgggcaatct gcaaacgctg ctgcataccg ccggaaaagg tggtcggcag
     4921 gtcgtcgatc cggttggcgg gaatctccac ctcttccagc cacttctgcg cggtggcacg
     4981 aatatcgccg taatgacgcg ccccggtcgc catcagccgc tcgccgatat tgccgcctgc
     5041 cgagacctgg cggcgcaggc cgtcgagcgg atgctgatgc accacgcccc attcggtgcg
     5101 cagcaggcga cggcggtcgg cctcgctcat gccatagagg gaacggtttt catagtgaat
     5161 ctccccctgc tgcggcgtca gacgcgcgga gatcgacttc agcagcgtgg tcttcccgga
     5221 gccggactcc ccgacaatgc ccagcacttc ccccggccat aaatcaaaag agacatcact
     5281 aaagcctttg cccggcgcgt aaaggtgggt caggttattg accgaaagta acggttgatt
     5341 cattggcttt tcgcctcgct ctgttggcgg caataatcgg tatcggagca gacaaacatg
     5401 cggtttccgg cgtcatccag caccacttca tcaagatagc tgtgggtcga accgcagatg
     5461 gcgcacggct catcccactg ctgaacggtg aacgggtgat cgtcgaaatc gagactttcc
     5521 acgcgggtaa acggcggcac cgcatagatg cgcttttcgc gccctgcgcc gaacagttgc
     5581 agggcgggca tcatgtccat ttttgggtta tcgaatttcg ggatcggcga cgggtccatc
     5641 acgtagcgcc catttacctt caccggatag gcgtaggtgg tggcgatatg accgaagcgg
     5701 gcaatatctt catacagttt cacctgcatc acgccgtact cttccagcgc gtgcatggtg
     5761 cgggtttccg tttcgcgcgg ctcgataaag cgcagcggtt cggggatcgg cacctggaag
     5821 ataatgatct gatcttccgt cagcggcgtt tcgggaatgc ggtggcgcgt ctggataagc
     5881 gtcgcatcat ccgtacgttc agtggtgttc accccggtca cgcgcttaaa gaagttgcga
     5941 atcgacacgg cgttggtggt gtcatccgcg ccctggtcaa tcaccttcaa cacgtcgctt
     6001 tcgccaatca cgctggcggt gagctgtatg ccgccggttc cccagccgta tggcatgggc
     6061 atctcgcgcc cgccaaacgg cacctgataa ccggggatcg ccaccgcttt taagatggcg
     6121 cggcggatca tgcgtttggt ctgctcgtcg aggtaggcaa agttgtagcc gctcagatta
     6181 gccatggttc tgctcctgtt gcagacgttt gagtagctcc agttcggcct ggaaatcgac
     6241 gtagtgcggg agtttgaggt gcgagacaaa gcctgcggct tcgacattgt cggcatgtgc
     6301 cagcacgaac tcttcatcct gcgccgggcc tgtcgcgtgc tcgccgtact ccggggcttg
     6361 cagagcgcgg tcaaccagcg ccatcgccat cgctttgcgc tcgctcatgc cgaacaccag
     6421 cccgtagccg cgcgtgaagt acggcggttc atccggcggg tcgataaaac cgttaaccat
     6481 ttcacactcg gtcatcagca gttcgccgac gtttaccgca aatcccagct cttccggcac
     6541 aatcgatacg tcgatatagc cgctgcggat ctcgcccgcg aacgggtggt tgcgcccgta
     6601 accgcgttgg gtggagtagg ccagcgccag caaatagcct tcgtcgccgc gcatcaactg
     6661 ctgcaaacgg gaagaacgtg agcaggggta aaccggcggc gtgcgggtga tgtcatccgg
     6721 ctgtgcgccg ctatcctctt caaacttcgc cagcccctga cgcgccagca ggctgaaaac
     6781 gtgcggcgac ggctgttgtt cgctgtcggc ggtggtcagc gttggtgctt caccgtttgc
     6841 caacagggtg aaatcgagca ggcgatgggt gtagtcgtag gtagggccaa gcaactggcc
     6901 gccgggaatg tctttataaa ccgccgaaat acgccgttcg agacgcatcc cggtggtgtc
     6961 gagcggctcg cttaccgcca gcttcgccaa cgtggtgcgg taggcgcgca gcaggaaaat
     7021 cgcttcaacg ttatcgccgc tggcctgttt cagcgccagc gccgccagtt cgcggtcggc
     7081 aatgccgcct tcggtcatca cgcgatctac cgcgaggtta agctgctgtt caatctgggc
     7141 gacgctcagt tcaggcaaat cggtatcgcc ccggcgtcgg ctctcttgca gggcgtgggc
     7201 ggcgtcgatc gccttttcgc ccccttttac ggcaacgtac atcagcacac ctccacatgc
     7261 gtggttcgcg gaatagccag caggcgttcg ccgcaggtca ggatcaggtc gatgccgagc
     7321 gggaacgggt gcgggcgctc ggtgagttcg tgcagaatgc actccggcag ctgcggagcg
     7381 atcattcgtt cttcggcaat acccgcgccg gtgagacgca acatgcgccc gccgctcagg
     7441 ctggcgacct gtaaaatcag cgtcgcgccc gcttccggcg caacggcggt gccggtggaa
     7501 agggcgttga gctgttcgct ggaaatcgcc tcatccgtca ccgcgaaggt cgcctgttcc
     7561 ggctggctga ccagcggcgc gttggtatga aaacgcaggc tctggttgac gatatcgtta
     7621 tttaatgggg tagaaagcca caccggcgtg tcgttatcgg ccagcgtcag cagcacgctg
     7681 gtggtggcga tattcagcgg ttgccagccg cgtttgagct gatgcagggc gacaatcacg
     7741 cccggctcgc tcatggcctt taacaggcga cgaaaactgt gctgggcatc ctgcacggga
     7801 agcataaaag cggtttccag ggtcatgcgt tgtctccgcg aaccatcgta aagaagtcga
     7861 cccggctggc gttcacttcg gcctggcgtg cggcaatgcg tgccatacgg tcagcgtcca
     7921 gcggggcaat aagggtttct gataagtttt gaaagtggcg agattgctgc atcagcgcgt
     7981 caatcagcgc gcagcgttcg gcgtgctgtt tatcacgccc cagcacccag ctgtagccga
     8041 gcgtgccgtc agtcaggcgc acggcggcgc gggtcagcgt ggcgtcgccg gcaaaaaaac
     8101 gttcgccggt gccgcccatc cgcgcctgaa tctgtaccag gccagtttca gcggcgcgga
     8161 tcacctcata gtcggcggtg atgtttagcg cgttcaggcg tgctgccagt tcagccggtt
     8221 ggctgtgcgc cagcacggac atccagtgct ggcgggtcgc ggtatctgcg tgcattcagt
     8281 gctccatagt gaattcaatc atgtcggcgc gcgtcaggct gacggagtac tccgccgggc
     8341 tgctttcacc gtcacggtgg ttaagggtgc gcacgcacag cagcggcgac atattcggga
     8401 tttcaagacg ctggcactct ttggcctggg cgcggcgggc gctgatccgc gtctggctgc
     8461 ggcgcagcgc aattccggtt tgctcgcgca gaaaatcgtg cagcgagccg ctgtcgaagc
     8521 gttgcagcgt cggccagagg gtgaggtccg cgaagtagtg gtcgattaaa cagagcgcga
     8581 cgccgttgac ccgacgcagg gtgcgcaggt ggatgacgtt ctccccctcg gtaatcccca
     8641 gtgcgtcagc gacgtggccg gacgcggggc gcaataccga aagcagtttt tcgctggtgg
     8701 gatggctgcc ctgatccagc agattctggc taaaacgcgc ctgggcgttg agcgggtaat
     8761 cgaacgggcg catcagcacc agcacgccga cgccctgacg gcgctgtacc cagccttttt
     8821 ccaccagttg gtcgatggcg cggcgcaggg tgtggcgatt cacctcaaag cgcgctgcca
     8881 gttgctgctc ggcgggaaga tagtcgccgc agcggtagtg ttgacgaagc tcctgctcaa
     8941 gttttgcggc tatctcttga tagcgtgttg ggtagctggt cggatgtgta gacaagtgca
     9001 tagatatcaa tgcctcgctt atcagataaa gtgcttacgc aaccgttgag agaggaaatc
     9061 cagcaggctg accgtgacga tgataagcac catcagggcg caggtttgtt ggaactggaa
     9121 accgcgaatc gcttcccaca gggtgacgcc gatcccgccc gcgccgacca tgccgacgac
     9181 ggtcgccgag cggacgttgg attcgaagcg atagagggag taggagatca gcagcggcat
     9241 cacctgcggc agcacgccgt agaggatctc ttcgagcttg ttggcaccgg tggcgcgaat
     9301 gccttccacc gggccgggtt caatcgcttc caccgcttcg gaaagcagtt tggagagcac
     9361 gccggtggtg tgaatgaaca gcgccagcac gccagcgaac gggccgaggc cgacggcgac
     9421 cacgaacagc atggcgaaga ccatttcgtt aatggcgcgg caggcgtcca tcaggcggcg
     9481 aacgggctgg taaacccacc acggcaccag gttttcggcg ctcatcaggc caaaggggat
     9541 ggagagaacc accgccagcg cggtgcccca gacggcgatt tgcagcgtga cggccatttc
     9601 ggtgaggtaa tcctgccact ggctgaaatc gggcgggaag aagtcggcgg cgaacgtcgc
     9661 catgttgccg ccgtctttga tcagcgtaag cggggccatt tccgcgccct gccacgagac
     9721 gaccaacacg gcgagtacaa cggcccagct cagaagcgag aaccagctgc gcttgggtgg
     9781 ggcgatggtg atggtttgca tgtttggctc cggtctgtag gccggataag gtgcttgcac
     9841 cgcatccggc atcaacgcct gcacattgtc ggatgcgacg ccggggcgtc ttatccgacc
     9901 tacgatttac tgcaccgctt tactcaccga actcatcgcg cttagcgcgt tgttcaggcg
     9961 gtccaggtca tccagctgcg cctgaatctc ggtggttttt gccagcttgt cctgctcatt
    10021 cagtccttta ttgcttttca cgccctgcat ctctttaaac agcgcgagct ggcgaatcgg
    10081 caccagttgc aggtcgctgg aagcgcggaa tggcgcccag cccaggcgtt ccagcaccgc
    10141 tttttcttcc ggcgttttgc cgtagttcat aaagaagtcg tagatcttgt ctttggtggt
    10201 ttcggaaaga ttcttgcgcc agacgatcgg atcgcctggg atcagcggcg acttccagat
    10261 caccttcagt tctttcagct tctctggcgc ggaggttttc agcttgtcga ggttttcggt
    10321 gttgttggtg gcaacatcca cctgcttgtt ggcgacggcc agcgcgttgg tttcatgccc
    10381 ggcgttgacg gtgcgcttaa agtcgctggc ggagatattg tttttggcaa agacgtagta
    10441 accggggacg aggaagccag aggtggagtt aggatcgcca ttgccgaagg tgagatcttt
    10501 ccgcttcgcc agcagatcgt tcaggttgtt gatcggacta tctttgttga cgatcaacac
    10561 gctccagtaa cccggcgatc cgtccgccgc gacggtctga gcgaagacct ggccgttggc
    10621 gcgatccacc gcttccattg ccgacaggtt gccgtaccag gcgatatcca ctttattgaa
    10681 gcgcattccc tggataatgc ctgcgtagtc tggggcaaag aaggcgttca ccttcacgcc
    10741 cagcttcttc tccatatcct gtaagaatgg cgtccattgc ggtttcaggt tttgctgtga
    10801 ttccgttgaa ataatgccga aattcagcgc cttttcctgc tcttcggcgt gtgccgggct
    10861 taacagggtg ctgaggctga acatgctggt gaaggccagc gaggcaatta tcttagcgtt
    10921 cattcgtttt cctcaatgat ggggatgtca ggcagctttc gcgttctctt cgatgcgatt
    10981 aatgctgcgg tagagatggt caaaacgttc gttatcaaac tgttggctgc tgccgtcgta
    11041 gaaaacgtgc ccctggcgca gggcgacgat gcgttcgcag tagcgcaggg cgtaatccac
    11101 ctgatgcagc gtgacgacca cggtgatgcc gtcgttctga ttgatgtcgc gcagggtgtc
    11161 catcacgatg cgggcggatt ccgggtccag cgaggcgatg ggttcatcgg ccagaatcac
    11221 cttcgcctgc tgcatcagcg cgcgggcaat cgccacacgc tgctgctgtc cgccggagag
    11281 ggtggaaacg cgttgatggg caaaatgcac catgccaacg caggtcagcg cctgtaacgc
    11341 gcgttgtttc tgctcgcggg taaaccagct aaaacaggtg cgccagaacg gcgtgctgcc
    11401 gagcgcgcca atcagcacgt tctccagtac gctcaggcgg ttcaccaggt tgaattgttg
    11461 gaagatgtag ccggtgttgg cgcggctttt gcggatatcg cgcgccagac ggccttcgcg
    11521 ctggactgtg cggcccagca gctcgatata gctgccggcg gatttatcgc cggtaatcaa
    11581 accgcttaag tgacgtaaaa gggtggattt gccggaaccc gacggcccaa gcagagccac
    11641 catttcaccg tgatgaatgt tcagatcaac cgcatgcagc gcctgatgct gattgaaggt
    11701 tttggcgagc ttctcgacac ggataatcgt ttgcatgatg cagcctccct aaaaaagtgg
    11761 ctccatcgtg gcggattatt gtgacgattt ggttaattaa aagtgactaa cagatgaaga
    11821 gttaacggga attcgatgac agtgcgggag ggcgggcctc ccggcgggtt attgcgttgg
    11881 ttgttgtttg acgacattaa tcatccacgg tacgccaaat ttatcggtga ctttgccaaa
    11941 gccatgcgcc cagaaagttt cctgccaggc catttcgatt tttccgttag cggcaagatt
    12001 gtcaaaccag cgttttcctt cttcgacctg ttgcgaatcg agcaccagcg taaagccgga
    12061 gtagctggct tttcctgacg gcatggcatc gctcatcatg atgtcgcttc cggcaatgcg
    12121 cacgttggca tgagcgatgg cggtatcggg aaattgcatt ccggaagggc agttctcggc
    12181 gctgtcctgc gctgattttg gcatttcgcc gaagctgatt ttatagagca gttccgcgcc
    12241 caacgtacgt tgataatagg caatcgcgtc ggaacagtta ccggcaaaag agaggtaggg
    12301 acttaacggc atgatggtga cctcagttaa gagaagccag ttaagtgtag ttcgaaattt
    12361 atacagatga gaggcggcct gataagacgc gcgagcgtcg catcaggcag tcggcactgt
    12421 tgccggatgc ggcgtaaacg ccttatccgg cctacggagg gtgcgggaat ttgtaggcct
    12481 gataagacgc gcaagcgtcg catcaggcat ctggcatcgt tgccggatgc ggcgcgagcg
    12541 ccttatccgg cttaccgatc acaatacaat cagttctttt tcacaaactc agatttcagt
    12601 ttcatcggac caaaaccgtc gattttgcaa tcgatgttat ggtcgccttc aaccaggcgg
    12661 atgtttttca ctttggtgcc aattttcagc atcgaagagc tacctttcac cttcagatct
    12721 ttaatgatgg taacgctgtc gccgtcagcc agcagattgc cgttagcatc tttaacgatc
    12781 agctcgtcgc tttcctgtgc aggttctgcg tcgttccatt cgtaggcaca ttccgggcag
    12841 atgtacatgc cgttatcttc gtaagtgtat tcggagttgc attttgggca gtgtggtaat
    12901 gacatgtgga tttcctcaaa agtcagcagg gcaaaagcgc caaaaaacgg cagattgccg
    12961 aaaaaggccg caattataca caaaatccct gagtttgtcg ggggtactgc tgattttaga
    13021 cgacgatcgt tttcatttga aaggaaacgc aaaaagaaaa taatcttttt gcctccattt
    13081 attatttaag ataaggtta
//
LOCUS       DAOFQD010000129        13087 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.129, whole genome shotgun sequence.
ACCESSION   DAOFQD010000129 DAOFQD010000000
VERSION     DAOFQD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.129"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(104..1753)
                     /gene="pgi"
                     /locus_tag="QBF46_003854"
     CDS             complement(104..1753)
                     /gene="pgi"
                     /locus_tag="QBF46_003854"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDQ6675981.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2278..3627
                     /gene="lysC"
                     /locus_tag="QBF46_003855"
     CDS             2278..3627
                     /gene="lysC"
                     /locus_tag="QBF46_003855"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDQ6675982.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3880..4152
                     /locus_tag="QBF46_003856"
     CDS             3880..4152
                     /locus_tag="QBF46_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDQ6675983.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     DYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4285..5157)
                     /gene="rluF"
                     /locus_tag="QBF46_003857"
     CDS             complement(4285..5157)
                     /gene="rluF"
                     /locus_tag="QBF46_003857"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDQ6675984.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5369..6058
                     /gene="pepE"
                     /locus_tag="QBF46_003858"
     CDS             5369..6058
                     /gene="pepE"
                     /locus_tag="QBF46_003858"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDQ6675985.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6149..7780)
                     /locus_tag="QBF46_003859"
     CDS             complement(6149..7780)
                     /locus_tag="QBF46_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDQ6675986.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(8000..11683)
                     /gene="metH"
                     /locus_tag="QBF46_003860"
     CDS             complement(8000..11683)
                     /gene="metH"
                     /locus_tag="QBF46_003860"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDQ6675987.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDGFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            11883..12707
                     /gene="iclR"
                     /locus_tag="QBF46_003861"
     CDS             11883..12707
                     /gene="iclR"
                     /locus_tag="QBF46_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDQ6675988.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3028 a         3507 c         3361 g         3191 t
ORIGIN      
        1 tcggcatcga cctgtaggcc tgataagacg cgaccgcgtc gcatcaggca tcggttgccg
       61 gatgcggcgt gaacgcctta tccggcctac atatcgacga tgattaaccg cgccacgctt
      121 tatagcggtt aatcagacca ttggtcgaac tatcgtggct gctgatttct ttatcatctt
      181 tcagctctgg cagaatacgg ttcgccagct gtttacccag ttccacgccc cactggtcga
      241 aggtgaagat gttcaggatc acgccctgag taaagatttt gtgctcatac agcgcaatca
      301 acgcacccag gctgaacgga gtgatttcac gcagcaggat ggagttggtc gggcggttac
      361 cttcgaatac tttgaacggc accacgtagt caagcgttgc cggatcttta ccctgatcac
      421 gatattcctg ctcaaccact tcgcgggatt taccaaacgc cagcgcttcg gtctgggcga
      481 agaagttaga cagcagtttc tggtgatgat cagagagcgg gttatgggtg atagccggag
      541 cgatgaaatc gcacggtacc attttggttc cctggtggat cagctggtag aacgcgtgct
      601 gaccgttagt gcctggttca ccccagataa tcgggccagt ctggtaatcc acaacgttac
      661 cgttacggtc aacatactta ccgttggact ccatattgcc ctgctggaag tacgccgcga
      721 aacggtgcat atactggtca tacggcagaa tcgcttcagt ttccgcacca aagaaattgt
      781 tgtaccagat gccaatcagc gccagcagta caggcaggtt tttctcggca ggcgtggtgg
      841 agaaatgctt gtccatcgcg tgtgcgccgg aaagcagttc aacgaagtta tcaaagccga
      901 tggagagaac aatcgacagg ccaatcgctg accacaaaga gtaacggccg ccaacccagt
      961 cccagaactc gaacatgttg gcagtatcaa taccaaactc gccaacggct ttggcattgg
     1021 tggaaagcgc cgcaaagtgt tttgcaacgt gtttttcatc acctgccgct ttcaggaacc
     1081 agtcacgcgc gctatgggcg ttggtcatag tttcctgagt ggtgaaggtt ttagatgcta
     1141 ccaggaacag cgtggtttcc gggtttactt ttttcagcac ttccgcgatg tgagtcccat
     1201 cgacgttaga aacaaagtgc atgttcaggt ggtttttgta cggacgcaga gcttcggtca
     1261 ccatgtatgg gccgaggtca gaaccgccga tcccgatgtt cactacgtca gtgattgctt
     1321 tgccggtata acctttccac tcaccggaaa taatcgcttc tgagaaggtt ttcatcttct
     1381 ccagcaccgc gttgacttcc ggcattacgt ctttgccatc aaccaaaatc ggggtattgc
     1441 tacggttacg cagcgctacg tgcagcacgg cgcggttttc agtgcggttg atcttctcgc
     1501 cagagaacat cgacttaatc gcgcccgcca gatcgcactc tttcgccaga tcctgtaatt
     1561 tcgccagcgt ctcttcagtg atgcggtttt tggagtaatc caccagcatc tgatcgtcga
     1621 aggttgcgga gaacttagaa aaacgatcgc cgtctttagc aaaaagatcg gcgatcgtaa
     1681 cgtctttcat ttcatcgaag tgtttctgta gtgcctgcca ggcagcggtc tgcgttggat
     1741 tgatgttttt cattagcaat actcttctga ttttgagaat tgtgactttg gaagattgta
     1801 gcgccagtca cagaaaaatg tgatggtttt agtgccgtta gcgtaatgtt gagtgtaaac
     1861 ccttagcgca gtgaagcatt tattagctga actactgacc gccaggagtg gatgaaaaat
     1921 ccgcatgacc ccatcgttga caaccgcccc gctcaccctt tatttataaa tgtactacct
     1981 gcgctagcgc aggccagaag aggcgcgttg cccaagtaac ggtgttggag gagccagtcc
     2041 tgtgataaca cctgaggggg tgcatcgccg aggtgattga acggctggcc acgttcatca
     2101 tcggctacag gggctgaatc ccctgggttg tcaccagaag cgttcgcagt cgggcgtttc
     2161 gcaagtggtg gagcacttct gggtgaaaat agtagcgaag tatcgctctg cgcccacccg
     2221 tcttccgctc ttcccttgtg ccaaggctga aaatggatcc cctgacacga ggtagttatg
     2281 tctgaaattg ttgtctccaa atttggcggt accagcgtag ctgattttga cgccatgaac
     2341 cgcagcgctg atattgtgct ttctgatgcc aacgtgcgtt tagttgtcct ctcggcttct
     2401 gctggtatca ctaatctgct ggtcgcttta gctgaaggac tggaacctgg cgagcgattc
     2461 gaaaaactcg acgctatccg caacatccag tttgccattc tggaacgtct gcgttacccg
     2521 aacgttatcc gtgaagagat tgaacgtctg ctggagaaca ttactgttct ggcagaagcg
     2581 gcggcgctgg caacgtctcc ggcgctgaca gatgagctgg tcagccacgg cgagctgatg
     2641 tcgaccctgc tgtttgttga gatcctgcgc gaacgcgatg ttcaggcaca gtggtttgat
     2701 gtacgtaaag tgatgcgtac caacgaccga tttggtcgtg cagagccaga tatagccgcg
     2761 ctggcggaac tggccgcgct gcagctgctc ccacgtctca atgaaggctt agtgatcacc
     2821 cagggattta tcggtagcga aaataaaggt cgtacaacga cgcttggccg tggaggcagc
     2881 gattatacgg cagccttgct ggcggaggct ttacacgcat ctcgtgttga tatctggacc
     2941 gacgtcccgg gcatctacac caccgatcca cgcgtagttt ccgcagcaaa acgcattgat
     3001 gaaatcgcgt ttgccgaagc ggcagagatg gcaacttttg gtgcaaaagt actgcatccg
     3061 gcaacgttgc tacccgcagt acgcagcgat atcccggtct ttgtcggctc cagcaaagac
     3121 ccacgcgcag gtggtacgct ggtgtgcaat aaaactgaaa atccgccgct gttccgcgct
     3181 ctggcgcttc gtcgcaatca gactctgctc actttgcaca gcctgaatat gctgcattct
     3241 cgcggtttcc tcgcggaagt tttcggcatc ctcgcgcggc ataatatttc ggtagactta
     3301 atcaccacgt cagaagtgag cgtggcatta acccttgata ccaccggttc aacctccact
     3361 ggcgatacgt tgctgacgca atctctgctg atggagcttt ccgcactgtg ccgggtggaa
     3421 gtagaagaag gtctggcgct ggtcgcgttg attggcaatg acctgtcaaa agcctgcggc
     3481 gttggcaaag aggtattcgg cgtactggaa ccgttcaaca ttcgcatgat ttgttatggc
     3541 gcatccagcc ataacctgtg cttcctggtg cccggcgaag atgccgagca ggtggtgcaa
     3601 aaactgcata gtaatttgtt tgagtaaata ctgtatggcc tggaagctat atttcgggcc
     3661 gtattgattt tcttgtcact atgctcatca ataaacgagc ctgtactctg ttaaccagcg
     3721 tctttatcgg agaataattg cctttaattt ttttatctgc atctctaatt aattatcgaa
     3781 agagataaat agttaagaga aggcaaaatg aatattatca gttctgctcg caaacgaatt
     3841 cccgtacact gaactctctg gctcactgag ggcaccatca tggcactccc ccgtattacc
     3901 caaaaagaga tgactgaacg cgagcagcgt gaacttaaaa cactgctgga tcgggcgcgg
     3961 attgcgcatg gtcgcgtatt gaccaactcc gaaaccaaca gcattaagaa agattacatc
     4021 gataaattaa tggttgagcg tgaggctgaa gcgaaaaaag cccgccagtt gaagaaaaag
     4081 caggcttata aaccggatcc tgaagcgtca tttagctggt cagcaaatac gtcgacgcgc
     4141 ggcaggcgct aatccacaat caacgcctga gattttcact cgtcagtgtg aacacaattt
     4201 tcccctcacc ctaacccttt tcccgaaggg gcgaggggac tgtccgggca cttttgggcc
     4261 gttttcatca gtctgaggcg ttaatcagcg ccccttcttt ttacgccccg gcgaggtaaa
     4321 acgcttaccg ttggacgccg ggcgaccgcc tttttccgcc gttttttcca tcttaacgac
     4381 tggacgtttg atgcccgctg ttttcggttt ggccttcgct ttaggtttta cctcggaaga
     4441 ggaattttca atgagcttaa agaggtcgat taactcatcg tcggttaaat cgcgccattc
     4501 ccccagcgga atgccgctta agctaacgtt catgatgcgc gtgcgttcca gctttttcac
     4561 ttcatagccg aaatgctcgc acatgcgccg gatctgacgg ttcagcccct gcaccagggt
     4621 aatgcggaag acaaacggcg cttctttttt gactttgcac tttttagtca ccgtcccgag
     4681 gatcggcacc cccgccccca taccacgaat aaattcatcg gtaatcggtt tatcgaccgt
     4741 caccagatac tctttctcat gatcgttacc cgcacggagg atcttattca ccagatcgcc
     4801 gtgattggtg aggaaaatca gcccctggga gtctttatcc aggcggccaa tcgggaacac
     4861 gcgtttgctg tggttaacga aatcgacaat gttatcgcgc tcgccatctt cggtggtgct
     4921 gacaataccg acgggcttgt tcagggcgat aagtaccaaa tcttcggctt cccgaggctc
     4981 aatcaactga ccgtttactt tcacaacgtc gccgggtttc acctgatcgc caatggtggc
     5041 tcgcttgcca ttaaggaaca cattgccttg ctcgatatag cgatccgctt cgcggcgtga
     5101 gcaaattccg ctttcgctga tgtatttatt taaacggact gatgagtcgg gcagcataaa
     5161 ttctcctgta aaaaacggaa tataccgtac ctttgggttg ataaaaaata gtcatgagcg
     5221 aactatttgt tatttccatt ggcttgaatt cgttacctgc tcaaatcact ttccagaagc
     5281 aatgtgcgac atcgtgagcc atgtcagtaa gtggttaaat tcgcagtggt agcatgcctc
     5341 ccgtgatttc ttactaaccg gagaagtaat ggaactgctt ttattgagta actcgacgct
     5401 gccgggtaaa gcctggctgg aacatgcact gccgctaatt gctgaacagt tgcagggtcg
     5461 ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag acctgggatg attacacagc
     5521 gaaaacggct gcggttctcg ctccgctggg tgtttctgtc accggtattc atagcgttgt
     5581 cgatcccgtt gccgcgattg aaaatgctga gatcgtgatt gtcggcggcg ggaatacttt
     5641 ccagttgctg aaacagtgcc gcgagcgcgg gctgctggca ccaattactg acgtggttaa
     5701 acgtggcgct ctgtatattg gctggagcgc aggcgctaac cttgcttgcc caactattcg
     5761 taccaccaac gatatgccga ttgtcgatcc gcaaggtttc gatgcgctaa atctgttccc
     5821 gctgcaaatc aacccgcact tcaccaacgc gctgccggaa ggccataaag gtgaaacccg
     5881 tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa ctgacgatta ttggtctacc
     5941 ggaaggtaac tggatcacag tgagtaaagg tcacgctacg ctgggtggcc cgaacaccac
     6001 ttatgtgttt aaggctggtg aagaagcggt tccgctggaa gctggtcacc gtttttaagt
     6061 gaattgccgg atgcatgatc cggcatcttg ttcatagtgc ctgatgcgac gcttgacgcg
     6121 tcttatcagg cctacaggat tcacaatatt aatactcgtc ccgtccttca tcttcatccg
     6181 gctgttccag cacactgtaa gccaccgaac aaaacagcga gttcaggcgc tgcatatctc
     6241 ccagtaagcc taaatgtagc gaactggttt caatgctctg cacgttttgc tgatgcaggc
     6301 gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg aaaacgatgt ttgctgcgac
     6361 gcaaacgacg agcgctggtg acatcgccag agaagaacac cgacattgcc agctttaaat
     6421 tactgagcaa ttgctcatag agcgcatcca gttccttcaa cccatcaagc gaaaatgccc
     6481 gccgtgctgc cagtgatttg tcagcaattt cgctgcccat gcgctcgacg atatcggagg
     6541 cctgttcaag gttgagcgac atttcgatga tctctgccca gcggcgcgac tcttcctccg
     6601 ccagctcttc ttttggcatc cgcgccagat acagcttaat ggcggtgtag agaacgttga
     6661 tatcatctgc cagcttacgc agctctttct cctgccgtgg ctcaccgtgc atcactttat
     6721 tcaacccttc catcatctgt tccatggcgt cgccaatgcg cagggtttcg cgcgcggcgt
     6781 tcgccagagc aagcgtgggc gtatccagcg cgctgacatc cagatgttta ggccgtagct
     6841 gggtatccag ttccggttca tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa
     6901 caaatggcag catgaccagg caacgtacaa ggttgtagaa gacgtggaaa tagatcacca
     6961 gttccgcttt tggcaatgac aacttcccca ttgtctctgc cagcaaatgg acaaacggca
     7021 ggataatcag gctacccacc agcttaaaca gcagactacc cagcgcgaca cggcgggctg
     7081 cggcattggc ggcactgttg ttgagcattg ccagcagacc ggaaccgagg ttagcaccaa
     7141 tcaccagaca gagcgccacg gggaaggaga taatgcctgc ggcggtcaga gtcgcggtca
     7201 gcagtacagc agcaaggctg gagtagctga taatggcgaa catcgcgcca atcagcgcat
     7261 ccagcagaat atcgccggtc agcgaggcaa agatcacctg aacgccgttt gcctgggtga
     7321 tcggcgttac ggcctgcaca atcaactcca gcgctagcaa aatcagccca agaccaatac
     7381 cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga
     7441 taaaaataag taacggtgac agccaggata agtcgaaggt gagaatacgc gccattagcg
     7501 ccgtcccgac atctgcaccc agcacaatga ccagagccgg tgcgagggct accagatcct
     7561 gagcgacaaa cgaggtcacc agcatggtgg tggcattact gctctgtacc agtgcggtaa
     7621 cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac gctccggcta aggacagtac
     7681 gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac aatatgagtc ccccagacca
     7741 gcagggcgac ggcagaaagc aggtgaagca gcgttaacac gagttcaggt tctccttatc
     7801 gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg aaacacgctg actggcatct
     7861 ttactgtgcc ttttgacatt cgtttttccg gctaagatta ttccagcctg tttccagtat
     7921 aagtgtttca tattaataaa gagacagggc actcattgag cgccctgttt gttgtaagga
     7981 aaagtgacag atttgtgaat cagtccgcgt cataccccag attcggtgcc agccagcgct
     8041 caacttcggt aacgctcata cctttacggc gggcataatc ttcaacctga tcgcgctgaa
     8101 tttgtgctac agcgtagtac ttgctgtccg ggtggctgaa gtaccaaccc gaaaccgatg
     8161 caccgggcca catggcgaaa gattctgtga gtttcatgcc agtgtgtttt tccacttcca
     8221 gcagctccca gatggtggct ttttccgtat gttccgggca ggccggatag cccggtgccg
     8281 gacggatgcc ctggtagttt tcgcggatca gctcttcgtt gctgaggttc tcgttcggcg
     8341 catagcccca gtagacttta cgcacacgct catggagata ctccgcaaag gcttcggcta
     8401 aacggtcggc aagcgctttc accatgattt tgttgtaatc atcgtgctgc gcttcaaagg
     8461 catcagccag tgcgtcctct tccagcccgc cagtcacggc aaatgcgccg atgtaatctg
     8521 ctttaccaga aagcttcggc gcaacgaagt cagcgagaca gtagttagcg aagcctgttt
     8581 tttcggtctg ttgacgcaga tggtggctga cgttgatcac atgggtacgc gtttcgtcac
     8641 ggtagatttc aatgtcatcg cccacacggt ttgccgggaa caggcccacc acgccacgcg
     8701 gattcagcgt tttctcggcg cttaatttat ccagcatgtc gttggcgtct ttaaacagcc
     8761 gctgcgcctc aacgcccacc acttcatctt ccagaatgcg cggatacttc ccggccagcg
     8821 accaggtcat aaagaacggt gtccagtcga tgtaattacg cagcgtttcg atgctggctt
     8881 cgacttcctg cacgccgaga cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaaag
     8941 cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg tgtgcgcggt ttcttgcgcc
     9001 cgtgctgaat acgtacggtt tcgtactcct tgcgggtacg agcgacaaaa ccatcacgct
     9061 gggtatcgga aagcagcgcc gccaccacac caacggtacg cgaggcattc tgcacataca
     9121 ccgtcgggcc gctgtagttc tgctcgattt tcaccgccgt gtgcgctttt gaggtcgtcg
     9181 cgccgccaat caatagcgga atagtgaagc cctgacgctc catctctttc gccacgttaa
     9241 ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc aatcaaatca gcattcactt
     9301 ctttggcggt acggagaatt ttttccgcag gcaccatcac accgagatcg acaatttcgt
     9361 agttgttaca ttgcagcacc acgccaacga tatttttacc gatgtcgtgg acatcgcctt
     9421 tcacggtggc gatcaccatc ttgccgttgg ttttaccctg ctctttgctg gcttcaataa
     9481 acggttcgag atacgccact gcctgtttca tgacgcgcgc cgatttgacc acctgtggca
     9541 ggaacatttt cccttcgcca aacagatcgc cgaccacgtt cataccgtcc atcagcgggc
     9601 cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc ttcttcggta tcctgctcga
     9661 taaactcggt aatgccttta accagcgagt attccagacg tttattcact tcccacgagc
     9721 gccactccgc ctgctgggcg ttagcggtgt cgtcggtttt gctgccgcga tatttctcgg
     9781 caagctccag taaacgctca gtgccatcgt cgcgacggtt aagaatcaca tcttccaccg
     9841 cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag ttgcccggcg ttgacgatcc
     9901 ccatatccat gccattgcga atagcgtagt agaggaacac cgcgtgaatg gcttcgcgca
     9961 ccggatcgtt gccacggaat gagaaagaaa cgttagatac accgccggaa atcagcgcgt
    10021 gcggcagttc gcgtttgata tcttcacacg cgccgataaa gtcctgcgcg tagttgttgt
    10081 gctcttcaat gccggttgcg accgcgaaga tgttcgggtc gaagatgata tcttctggcg
    10141 ggaagccaac ctcttcggtg aggattttgt acgcccgacg gcaaatctcg attttccgtg
    10201 cgcgggtatc ggcctgtccc tgttcgtcaa aggccattac caccactgcc gcaccgtaac
    10261 gacgcaagag cttcgcgtga tggataaagg cgtcgacgcc ctctttcatc gagatagagt
    10321 taacaatgcc tttgccctgg atacacttca gacctttttc aatgacatcc cattttgagg
    10381 agtcgatcat aatcggcacg cgagcgatat ccggttcacc ggcaatcaga ttgagaaaac
    10441 gcaccatcgc cgcttcggcg tcgagcatcc cttcatccat gttgatatcg ataatctgcg
    10501 caccgttttc cacctgctgg cgggccacat ccagtgcctc gctgtatttc tcttctttga
    10561 tcaggcgctt gaacttagcg gaaccggtga cgttggtgcg ttcacccacg ttcacaaaca
    10621 ggctatcttc gccaatgttc agcggctcca ggccggacaa acggcaggct acggggattt
    10681 ccggcagttt gcgcggcgct aatccttcta ctgcacgact catcgctgca atatgttgtg
    10741 gcgtggtgcc acagcagccg ccgacgatat tgagaaaacc cgcttgcgcc cattcacgta
    10801 tctgttttgc catcgtgtcg gcgtcgagat cgtactcacc aaaggcgttg ggtagcccgg
    10861 cgttcgggtg tgcggtgacg tagcattccg caatccgtga cagctcctgc acgtactggc
    10921 gcagttcatc gggccccagc gcacagttca ggccaaaggt cagagcttcg gcgtggcgca
    10981 atgagttgta aaatgcttcg gtggtctgcc cggagagcgt gcgcccggag gcgtcggtga
    11041 tggtgccgga gatcataatc ggcagctcaa cgcccagcgc ttcaaactcc gttttcaccg
    11101 caaaaaccgc cgctttggcg ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg
    11161 cgccaccttc caccagcgct ttggtggact ctcgataagc cgccaccagc ccgtcaaaag
    11221 tgatattacg aaatgccgga tcatttacgt ccggagaaat agacgccgtg cggttggtcg
    11281 ggccgagaac accggcaacg tagcgcggtt tctccggcgt gcgcgcggtc cactcatcag
    11341 cacaagctcg cgccagtttc gccgccgcaa agttgatttc cgccgacagg gattccatct
    11401 ggtaatccgc catcgcaatg gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc
    11461 ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc cggtttgctg agcaccagca
    11521 ggtcgttgtt gcctttgaga tcgcatggcc agtcggcaaa gcgttcacca cgaaaatcgg
    11581 cttcgttcag tcgatagctc tggatcatgg tgcccatacc gccgtccagc accagaatac
    11641 gttcatttaa ctgcgcacgc agttgttcca ctttgctgct cacacttgct cccgacacgc
    11701 tcaacccaga tttaataaaa attcaacaaa ccatactggc ataaacgcat ctgtggtaaa
    11761 agcgaccacc acgcaacatg agatttgttc aacattaact catcggatca gttcagcaac
    11821 tattgcatta gctaacaata aaaatgaaaa tgatttccac gatacagaaa aaggaggctg
    11881 tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc cgccgttgcc accgcaccag
    11941 cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt actggagtgg attgccgaat
    12001 ccaatggcag tgtggcactc acagagctgg cgcaacaagc cgggttaccc aattccacga
    12061 cccaccgcct gctaaccacg atgcaacagc agggtttcgt gcgtcaggtc ggcgaactgg
    12121 gacattgggc aatcggcgca catgccttta tggtcggcag cagctttctc cagagccgta
    12181 atttgttagc gattgttcac cctatcctgc gcaatttaat ggaagagtct ggcgaaacgg
    12241 tcaatatggc ggtgcttgat caaagcgatc acgaagcgat tattatcgac caggtacagt
    12301 gtacgcatct gatgcgaatg tccgcgccta tcggcggtaa attgccgatg cacgcttccg
    12361 gtgcgggtaa agccttttta gcccaactga gcgaagaaca ggtgacgaag ctgctgcacc
    12421 gcaaagggtt acatgcctat acccacgcaa cgctggtgtc tcctgtgcat ttaaaagaag
    12481 atctcgccca aacgcgcaaa cggggttatt catttgacga tgaggaacat gcactggggc
    12541 tacgttgcct tgcagcgtgt attttcgatg agcaccgcga accgtttgcc gcaatttcaa
    12601 tttccggacc gatttcacgt attaccgatg accgcgtgac cgagtttggc gcgatggtga
    12661 ttaaagcggc gaaggaagtg acgctggcgt acggtggaat gcgctgactt tttctggcgg
    12721 gcagaggcaa tattctgccc atcatacctg agtggcaata gaataagggt gtctgttaat
    12781 cgcattgacg ccaaaataac ttaatgtcat acacttcaca cacttttatt aactcagcat
    12841 tatttttaaa catcaaacca cttaatcatt aacaaaatcg caagatctca ttccaccgaa
    12901 acttttacgg ctgcattatg agattgagcg atttatgata tttctttagt tgtaatcata
    12961 ttaaattttt tcactgttct gatagttaaa attcaagaca tcaataaaca atgagatatt
    13021 taaatgatta ctcgcatccc tcgtagttct ttctctgcaa atattaataa tacagcccag
    13081 acaaatg
//
LOCUS       DAOFQD010000130        13037 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.130, whole genome shotgun sequence.
ACCESSION   DAOFQD010000130 DAOFQD010000000
VERSION     DAOFQD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.130"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            336..8316
                     /locus_tag="QBF46_003862"
                     /pseudo
     CDS             336..8316
                     /locus_tag="QBF46_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(8578..9630)
                     /locus_tag="QBF46_003863"
     CDS             complement(8578..9630)
                     /locus_tag="QBF46_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDQ6675989.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQKYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            9945..11261
                     /gene="shiA"
                     /locus_tag="QBF46_003864"
     CDS             9945..11261
                     /gene="shiA"
                     /locus_tag="QBF46_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDQ6675990.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFDHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            11363..12817
                     /gene="amn"
                     /locus_tag="QBF46_003865"
     CDS             11363..12817
                     /gene="amn"
                     /locus_tag="QBF46_003865"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDQ6675991.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         3548 a         3183 c         3098 g         3208 t
ORIGIN      
        1 tcgagtccag tcagaggagc caaattctaa aaattcgctt ttttagcgca atgtcactga
       61 ccttagttga acattgtttt ttaacggata gcgggttttt aacatcttaa gcgccctcga
      121 cctttatggt tgagggcgtt ttgctatgaa cgccatcacc attttcccct cgattataaa
      181 acttgagtta ttcagtagtc tcccctcttg caactcacac tcaaaactgc ctaacgaaaa
      241 gttattaatt ttcaatcata ttgctatcag gatttacatt ttttcgctgt gctagaaagg
      301 gcgcatttat gttagctcgt tcagggaagg taagcatggc tacgaagaag agaagtggag
      361 aagaaataaa tgaccgacaa atattatgcg ggatgggaat taaactacgc cgcttaactg
      421 cgggtatctg cctgataact caacttgcgt tccctatggc tgcggcagca caaggtgtgg
      481 taaacgccgc aacccaacaa ccagttcctg cacaattcgc cattgcaaat gccaatacgg
      541 taccctatac ccttggagcg ctggaatcgg cccaaagcgt tgccgaacgc ttcggtattt
      601 cggtggctga gttacgcaaa ctcaaccagt ttcgtacgtt tgctcgaggt tttgataatg
      661 tccgccaggg tgatgaactg gatgtcccgg cacaagttag tgaaaaaaaa ttaaccccgc
      721 cgccgggtaa tagcagtgac aacctcgagc aacagatagc cagtacttca cagcaaatcg
      781 ggtctctgct cgccgaagat atgaacagcg agcaagcggc aaatatggcg cgtggatggg
      841 cctcttctca ggcttcaggc gcaatgacag actggttaag ccgcttcggt accgcaagaa
      901 tcacgctggg cgtggatgaa gattttagcc tgaagaactc ccagttcgat tttctccatc
      961 cgtggtatga aacgcctgat aatctctttt tcagtcagca tactctccat cgtactaacg
     1021 agcgtacgca gattaacaac ggcttgggtt ggcgtcattt cactcccaca tggatgtcgg
     1081 gcatcaactt ctttttcgac cacgatctta gtcgttacca ctcccgcgcc ggcattggtg
     1141 cggagtactg gcgcgactat ctaaaattaa gcagtaacgg ctatttgcga ctgaccaact
     1201 ggcgcagcgc acctgaactg gacaacgatt atgaagcacg cccggccaat ggctgggatg
     1261 tacgcgcaga aggctggcta cccgcctggc cgcaccttgg cggtaaactg gtctatgaac
     1321 agtattatgg cgatgaagtg gccctgttcg ataaagacga tcggcaaagt aatcctcatg
     1381 ccataaccgc tggacttaac tataccccct tcccgctgat gaccttcagc gcggagcaac
     1441 gccagggtaa acagggcgaa aatgacaccc gttttgccgt cgattttacc tggcaacctg
     1501 gcagcgcaat gcagaaacag cttgacccga atgaagtcgc tgcacggcgt agccttgcag
     1561 gcagccgtta tgatctggtg gatcgcaaca acaatatcgt tctggaatat cgcaaaaaag
     1621 aactggttcg cctgaccctg acagaccccg tgacagggaa gtcaggagaa gtgaaatcac
     1681 tggtttcgtc gctacaaacc aaatatgccc tgaaaggcta taacgtcgaa gccaccgcac
     1741 tggaagctgc cggtggcaaa gtggtcacaa cgggtaaaga tattctggtt accctgccgg
     1801 cttaccggtt caccagtacg ccagaaaccg ataacacctg gccgattgaa gtcaccgccg
     1861 aagatttcaa aggcaatttg tcgaatcgtg aacagagcat ggtggtcgtt caggcaccta
     1921 cgctaagcca gaaagattcc tcggtatcgt taagtaccca aacattgaac gcggattccc
     1981 attcaaccgc cacactgact tttattgcgc atgatgcagc aggtaatcct gttgtcgggc
     2041 tggtgctctc gacgcgtcac gaaggtgttc aggacatcac cctttctgac tggaaagata
     2101 atggtgacgg aagctatacc cagatcctga ccacaggagc gatgtctggc acgctgacgc
     2161 tgatgccaca gctgaatggt gtggatgcgg ctaaagcccc cgccgtggtg aatatcattt
     2221 ctgtttcgtc atcccgaact cactcgtcaa ttaagattga taaggaccgt tatctctccg
     2281 gcaatcctat cgaggtgacg gtagaactga gagatgaaaa tgacaaacct gttaaggagc
     2341 aaaaagagca actgaatacc gcaatcagca tcgacaacgt gaaacctggt gtcactacag
     2401 actggaaaga aaccacagat ggcgtctata aggcaaccta taccgcctat accaaaggca
     2461 gtgggcttac tgcgaagcta ttaatgcaaa gctggaatga agatttgcat accgctggat
     2521 ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc agcaataatg
     2581 gtgtgctcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct gatgaaggaa
     2641 gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg gcaacttcct
     2701 ttaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactatt gatctgaaaa
     2761 gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg aaacaaacgt
     2821 taatcgtcag ttttgtcggc gactcgagta ccgcacaggt tgatctgcag aagtcgaaaa
     2881 atgaagtggt cgctgacggc aatgacagcg ccacaatgac cgctacagtt cgggatgcaa
     2941 aagcaacctg ctcaatgacg tcaaggtcac cttcaatgtt aattcagcag cagcgaaact
     3001 gagccaaacc gaagtgaata gccacgacgg gatcgccaca gctacgctga ccagtttgaa
     3061 aaatggtgat tatagggtta cggcctctgt gagctctggt tcccaggcta atcaacaggt
     3121 gaattttatt ggtgatcaaa gtactgctgc cctgaccctc agtgtgcctt caggtgatat
     3181 caccgtcacc aacacagctc cgcaatatat gactgcaacc ttgcaggata aaaatggcaa
     3241 cccactaaaa gataaagaaa tcaccttctc tgtgccaaac gacgtcgcaa gtaagttctc
     3301 gattagcaac ggaggaaaag gcatgacgga tagtaacggg gttgcaatcg cctccctgac
     3361 cggcacgtta gcgggcacgc atatgatcac ggctcgtctg gctaacagca atgtcagcga
     3421 tacacagcca atgacgtttg tggcggataa agacagagcg gttgtcgttc tgcaaacatc
     3481 gaaagcggaa atcattggga atggcgtgga tgagacgacg ctgacagcaa cagtgaaaga
     3541 tccttttgat aacgtggtta aaaatctgtc agtagccttc agcacctccc ccgcagacac
     3601 acaactgagc ctgaacgcgc gtaatactaa tgagaacggt attgctgaag ttacccttaa
     3661 gggcacggtt ttgggtgttc atacagccga agccacactg cctaacggca acaacgatac
     3721 gaagacagtc aatattgcgc ccgataccag caacgcgcag gtcaccctga acatccctgc
     3781 tcaacaggtg gtgacgaata acagcgacag tgtgcagctg acggcaacgg tgaaagatcc
     3841 gtcgaatcat cctgtggcgg gaataacggt gaacttcacc atgccacagg acgtggcggc
     3901 aaactttacc ctcgaaacta acggtattgc catcactcag gccaatggcg aagcgcatgt
     3961 caccctcaaa ggtaaaaaag cgggcacgca tacggttacc gcaacgttgg gtaacaataa
     4021 tgccagcgat gcgcaaccag tcacattcgt ggcggataag gacagcgcag ttgtcgttct
     4081 gcaaacatcg aaagcggaaa tcattgggaa tggcgtggat gagacgactc tgacggcaac
     4141 agtgaaagat ccttttgata acgcagtaaa agatctacag gtcaccttca gtaccaaccc
     4201 ggcagatact caacttagtc agagcacaag caatactaac gacagtggtg ttgctgaagt
     4261 tacccttaag ggcacggttt tgggcgttca tacagtcgaa gccacacttc ctaacggcaa
     4321 cggctataca acgacagtca atattgcgcc cgataccagc aacgcgcagg ttacgctgaa
     4381 catccctgct caacaggtgg tgacgaataa cagtgacagc gtgcagctgg cggcaacggt
     4441 gaaagatccg tcgaatcatc cggtggcggg aataacggtg accttcacca tgccacagga
     4501 cgtggcggca aactttaccc tcgaaaataa cggtattgcc atcacccagg ccaatgggga
     4561 agcgcatgtc acgctcaaag gtaaaaaagc gggcacgcat acggttaccg caacgctggg
     4621 taatacaata ccagtgattc gcagccagta acgtttgtgg cggacaaaac ctcggctcag
     4681 gttgtcctgc agatgtcaaa agatgagatc acaggtaatg gcgtcgataa cgcaacgcta
     4741 actgcaacgg ttaaagatca gttcgacaat gaggtgaata atcttccggt aacattcagc
     4801 tcagcctctt caggactcac cctgaccccg ggagtaagta ataccaatga gtctggcatc
     4861 gcgcaggcca ctctcgcagg cgttgccttt ggtgagcaga cggtcactgc atcactggct
     4921 aataatggtg ccagcgacaa caaaactgtg cattttattg gcgacacagc agcggcaaaa
     4981 attatcgagt tgacgcctgt cccagacagc ataatcgccg gtaccccgca gaacagctcc
     5041 ggcagcgtca tcaccgccac agtcgttgat aataatggct ttccggtgaa aggtgtgact
     5101 gtgaacttca ccagcagaac aaactctgcc gaaatgacga atggcggcca agccgtaacg
     5161 aacgaacagg gtaaggctac cgtcacttat accaataccc gctcctcgat agaatcagga
     5221 gcgagaccgg ataccgttga ggccagtctg gaaaatggta gctccacgct tagcacatca
     5281 attaatgtca acgctgatgc gtctacggca catctcacct tgctacaggc actttttgat
     5341 acagtctccg caggcgacac taccaatctg tatattgagg tgaaggataa ttacggcaac
     5401 ggtgtacccc agcaggaggt aaccctcaga gtatcaccaa gtgaaggcgt gacccccagt
     5461 aataacgcta tatatactac caaccacgac ggcaattttt acgcaagctt taccgctaca
     5521 aaagccgggg tttatcaagt gacggcaacc ctcgaaaatg gcgattcgat gcaacaaaca
     5581 gtgacctatg tgccgaacgt cgcgaatgcc gaaatcacgc tggcagcctc gaaggatccg
     5641 gtgattgccg acaataacga tctcacgaca ctaacagcaa cagtcgctga tacagagggc
     5701 aatgcgatag ccaacactga agtaacattt actctgccgg aagatgtgaa ggcgaacttc
     5761 acgctgagcg atggcggtaa agcgattact gatgctgaag gcaaagcgaa agtcacgctg
     5821 aaaggtacaa aagcaggcgc tcatactgtt acagcatcga tgactggcgg taagagtgag
     5881 cagttggtgg tgaactttat tgcggatacg ctcagtgcgc aggttaatct taacgttacc
     5941 gaggacaact ttatcgccaa taacgttggg atgaccacac ttcaggcaac agtgactgat
     6001 ggaaacggca acccgttagc caatgaggcg gtgacattca cgctaccggc agacgtgagc
     6061 gcaagcttca ctctcggaca aggcggttcc gccattactg atatcaacgg caaggctgaa
     6121 gttacactga gcggtacaaa atccggcacc taccccgtga cagttagcgt gaacaattat
     6181 ggtgtcagtg atacgaaaca ggtgactttg attgccgatg ctggtaccgc aacactagcc
     6241 tccttaacct ctgtatactc attcgtcgtc agcacgaccg agggcgcgac catgactgca
     6301 agcgtcactg acgctaacgg caacccggta gaaggcataa aagttaattt ccgcggaacc
     6361 tccgtcacgc taagcagcac cagcgttgaa acggatgatc agggtttcgc tgaaattctt
     6421 gtgacaagca ccgaagtcgg actgaaaaca gtttcagcct ctctggcaga taaacctact
     6481 gaagtcatat cgcgattact gaatgcaaaa gcagatatta attctgcaac gattaccagt
     6541 ctggagatac ctgaaggtca gctaatggtc gcacaagacg tagcagttaa agctcacgtc
     6601 aacgaccagt ttggcaaccc gattcttaat gaatctgtaa cattcagtgc agagccacca
     6661 gagcacatga ccatcagcca aaatattgtc tctactgata cgcatggtat agccgaggtc
     6721 tccatgacgc ccgaaagaaa cggttcgtat atggtgaaag catccctggc gaatggagcc
     6781 tcacttgaga aacaactgga ggctattgat gaaaaactga cactcacggc gtccagtccg
     6841 cttatcggtg tctatgcccc tacaggcact actctgacgg caacgctaac ctctgcaaat
     6901 ggcactccag tggagggtca ggtcatcaac tttagcgtaa cgccagaagg ggcgacgtta
     6961 agtggcggaa aagtgagaac taactcttca ggtcaggctc cggtcgttct gaccagcaat
     7021 aaagtcggta catatacggt gactgcatcg ttccataacg gcgtaacaat acagacacag
     7081 acaaccgtga aagtcactgg caactcaagc accgcacatg ttgctagctt tatcgctgat
     7141 ccatcgacta tcgccgccac caacagtgat ttaagtacct taaaggcaac ggttgaggat
     7201 ggcagtggta acctgatcga aggtctcact gtgtacttcg ccttaaaaag cggctctgcc
     7261 acattaacgt cattaacagc ggtgaccgat caaaacggaa tcgcgacaac aagcgtgaaa
     7321 ggagcgatga caggtagcgt cacggtaagc gcagtcacga ccgctggtgg aatgcaaaca
     7381 gtagatataa cgctggtggc aggcccggca gacgcctcgc agtccgtcct taagaacaat
     7441 cggtcctcac tgaaagggga ctttaccgat agtgctgagc tacatcttgt tttgcacgat
     7501 atatcaggca atccgatcaa agtttctgaa gggatggaat ttgtgcaatc aggtactaac
     7561 gtgccctata tgaaaattag cgcaattgat tacagtcaaa atatcaacgg tgattacaaa
     7621 gccaccatta caggcggcgg agagggtatc gcaacgctga tccctgtatt gaatggtgtt
     7681 catcaagctg gtctgagtac cacaatacaa ttcactcgcg cagaagacaa aataatgagc
     7741 ggtacagtat cagtcaatgg tactgaccta ccgacaacta cattcccttc gcaggggttc
     7801 accggggcgt attatcagtt gaataatgac aactttgccc caggaaaaac ggcggctgat
     7861 tatgagtttt caagctctgc ctcctgggtc gatgttgatg ctaccggtaa agtgacattt
     7921 aaaaatgtcg gcagcaattg ggaaaggatt acggcgacgc caaaatcagg aggccctagc
     7981 tatgtatacg aaatccgtgt gaagagttgg tgggtgaact ccggcgatgc tttcatgata
     8041 tacagccttg ctgaaaattt ttgcagtagc aatggctaca cacttccccg tgcagaccat
     8101 ttaaaccata gtcgttcccg aggcatcggg tcactgtaca gtgaatgggg agatatgggg
     8161 cattacacga ctgaagctgg ttttcaatca aatatgtatt ggtcatctag tcccgcaaac
     8221 tcaagcgaac aatacgtagt ttccctggca acaggtgatc aaagcgtatt tgaaaagctt
     8281 gggtttgctt atgcgacatg ttataaaaac ctgtgatttt ccgttataat ttcttaaaga
     8341 gtgctccatt cggagcactc ttttttacat tccctctata gagcttgccc ttctctcaca
     8401 gaaaatgaat aaataaaact gctgcaccgc aagaatcgcg ttcgatgtga ctatgatcgt
     8461 atccggtgag tacagctttg atccaaaggc tggttttctg tgatgattga ccagcctcag
     8521 agtattgatg acaccaccgt tcataacaga tcggcattac tatgcataag tactgtctca
     8581 tattctcgcc aaaacacttt ttaatgcctt tgcgaattca tccagactta tattgtcaat
     8641 tttttgccca acgacgactg actccgaaac atcggccatt gcgggccatc gcgttttgta
     8701 atcagcataa attgcaacaa cgggagtatt ataggcactt gccatatgta atattgaggt
     8761 gtcaggagtt attgcgatat aagcatcttt tatgattgct gctgaacgtt taatcgaggg
     8821 tgataaggat aaacggtaga cattattata acaatccact aataccctgg ctttatcttc
     8881 cccttttggt ccatgaacga tcactattgg tatatctgtt tcactttgaa tttttgctat
     8941 tagattttct gcaatcgata atgaaaatgt acgttcctgc gagctacctt ctaaattaag
     9001 cgcaatgtaa gatcctaacg atcgcatttc ctcgcgcacc tcatcgagta catcctcact
     9061 tagtggcaat tcatatattg gcctgactgc tggaaaaccc gcctcacgca tcataaatgc
     9121 ccatgtcatg ggtacaggag cccggagatt ctgatccatc cgggacgcgt tcttgcacaa
     9181 aggggagtag cacttcatgg ttaaaccaac aacctgaaaa ttcgtttttg ctttcaactg
     9241 actgataaat atcatcgttt tcaggttctt tttacgcatc gcctctatac aaagatccgg
     9301 cgtaccgtat ttctgtgtta tgttctttgc taaatctttt atttctttta atgttgcgtg
     9361 atcctgcata gtcattgtga ctaatgttaa tttagtcttt tcaagtttaa gtgcattaaa
     9421 cacttctaaa ttaattgtcg acgtaacaat taaaagatgc ttaattttat gcaattcaag
     9481 cgcccgaata acaggaaaga tggccatagc atcgccaatc tggtcgggaa tatggatgac
     9541 aacaaagtct gttttttcaa tattgaaatt ataagcttta taatcgtagt aactaaatgc
     9601 aatacgtctc aacaatgatg ctaaaaacat acctaacctc gcctccctac tggttataat
     9661 gcaatgcagt ctatcagact catcagggtg ccattttgtg catatgcgga cttttatgtt
     9721 tcatatctct aacctgtggg tcctctgctt aatccttaaa caacaccagc aactcctgcg
     9781 ctttcatctt ccatcgaatt tttcatgttg ccgctaatca gccataaaat catttgcaga
     9841 tgcgctctgt cgaggtagtc tcataaggtt cgtttataga tcgacggcaa tgtgagttac
     9901 cttttccata ctaattataa aaagacagta caaacaggat cattatggac tccacgctca
     9961 tctccactcg tcccgatgaa gggacgcttt cgttaagtcg cgcccgacga gctgcgttag
    10021 gcagcttcgc tggtgccgtc gtcgactggt atgatttttt actctatggc atcaccgccg
    10081 cactggtgtt taatcgcgag tttttcccgc aagtaagccc ggcgatggga acgctcgccg
    10141 catttgctac ctttggcgtc ggattccttt tccgtccgct cggcggtgtc attttcgatc
    10201 actttggcga ccgactggga cgtaagcgca tgttaatgct gaccgtctgg atgatgggca
    10261 tcgcgacagc cttgattggt attcttcctt cattctcgac cattgggtgg tgggcaccta
    10321 ttttgctggt gacactgcgt gccattcagg gatttgccgt cggcggcgaa tggggaggcg
    10381 cggcgttgct ttccgttgaa agtgcaccga aaaataaaaa agccttttac agtagcggtg
    10441 tacaagttgg ctacggtgta ggtttactgc tttcaaccgg actggtttca ttgatcagta
    10501 tgatgacgac tgacgaacag tttttaagct ggggctggcg cattcctttc ctgtttagca
    10561 tcgtactggt actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg
    10621 aacaacagca acattatcaa gctgccgcga aaaaacgcat cccggttatc gaagcgctgt
    10681 tacgacatcc cggtgctttc ctgaagatta ttgcgctacg actgtgcgaa ttgctgacga
    10741 tgtacatcgt tactgccttt gcacttaatt attcaaccca gaatatgggg ctaccgcgcg
    10801 aacttttcct taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt
    10861 ttgcctggct tgccgatcgt tttggtcgcc gtagggttta tatcacaggc gcgttaatcg
    10921 gaacgttgag cgcatttcct ttctttatgg cgcttgaagc acaatccatt ttctggatag
    10981 ttttcttctc cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac
    11041 cgatgtttac cgaaatgttt ggtgccagtt atcgctatag tggcgctgga gtcggttatc
    11101 aggttgccag tgtggttggc ggtggattta caccttttat tgccgctgca ctcatcactt
    11161 actttgccgg gaactggcat agcgtcgcca tttatttgct ggctggatgc ctgatttccg
    11221 caatgaccgc tttgttgatg aaagacagtc aacgcgcttg atagcctggc gaagatcatc
    11281 cgatcttcgc cttacacttt tgtttcacat ttctgtgaca tactatcgga tgtgcggtaa
    11341 ttgtatggaa caggagacac acatgaataa taagggctcc ggtctgaccc cagctcaggc
    11401 actggataaa ctcgacgcgc tgtatgagca atctgtagtc gcattacgca acgccattgg
    11461 caactatatt acaagtggcg aattacctga tgaaaacgcc cgcaaacaag gtctttttgt
    11521 ctatccatca ctgaccgtaa cctgggacgg tagcacaacc aatcccccca aaacgcgcgc
    11581 atttggtcgt tttactcacg caggcagcta caccaccacg attactcgcc ctactctctt
    11641 tcgttcgtat cttaatgaac aacttacgtt gctgtatcag gattatggtg cgcatatctc
    11701 tgtgcaaccc tcgcagcatg aaatccctta tccttatgtc atcgatggct ctgaattgac
    11761 acttgatcgc tcaatgagcg ctgggttaac tcgctacttc ccgacaacag aactggcgca
    11821 aattggcgat gaaactgcag acggcattta tcatccaacg gaattctccc cgctatcgca
    11881 ttttgatgcg cgccgcgtcg atttttccct cgcacggttg cgccattata ccggtacgcc
    11941 agttgaacat tttcagccgt tcgtcttgtt taccaactac acacgttatg tggatgaatt
    12001 cgttcgttgg ggatgcagcc agatcctcga tcctgatagt ccctacattg ccctttcttg
    12061 tgctggcggg aactggatca ccgccgaaac cgaagcgcca gaagaagcca tttccgacct
    12121 tgcatggaaa aaacatcaga tgccagcatg gcatttaatt accgccgatg gtcagggtat
    12181 tactctggtg aatattggcg tgggaccgtc aaatgctaaa accatctgcg atcatctggc
    12241 agtgctacgc ccggatgtct ggttgatgat tggtcactgt ggcggattac gtgaaagtca
    12301 ggccattggc gattatgtac ttgcacacgc ttatttacgc gatgaccacg ttcttgatgc
    12361 ggttctgccg cccgatattc ctattccgag cattgctgaa gtgcaacgtg cgctttatga
    12421 cgccaccaag ctggtgagcg gcaggcccgg tgaggaagtc aaacagcggc tacgtactgg
    12481 tactgtggta accacagatg acaggaactg ggaattacgt tactcagctt ctgcacttcg
    12541 ttttaactta agccgggccg tagcaattga tatggaaagt gcaaccattg ccgcgcaagg
    12601 atatcgtttc cgcgtgccat acgggacact actgtgtgtt tcagataaac cgttgcatgg
    12661 cgagattaaa cttcccggcc aggctaaccg tttttatgaa ggcgctattt ccgaacatct
    12721 gcaaattggc attcgggcga tcgatttgct gcgcgcagaa ggcgaccgac tgcattcgcg
    12781 taaattacga acctttaatg agccgccgtt ccgataataa gttctgaacc ttcttatcag
    12841 aaatagatgt gaaggagtaa gtaagacacc tggcaaatag cctgcaataa tcgtgggctg
    12901 tttgcttcct tgggcggata cgagttttat tatcgtctta atgatttcca catattaaaa
    12961 gcaagtatgc tttcaaaaca caattataaa aaatcccgcc aacaatataa gtttttataa
    13021 aattaaatat aagatta
//
LOCUS       DAOFQD010000131        12980 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.131, whole genome shotgun sequence.
ACCESSION   DAOFQD010000131 DAOFQD010000000
VERSION     DAOFQD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.131"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            204..308
                     /locus_tag="QBF46_003866"
     CDS             204..308
                     /locus_tag="QBF46_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDQ6675992.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            619..2817
                     /gene="speF"
                     /locus_tag="QBF46_003867"
     CDS             619..2817
                     /gene="speF"
                     /locus_tag="QBF46_003867"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HDQ6675993.1"
                     /translation="MSELKIAVSRSCPDCFSTHRTCVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERIPAEYLSRISGVFEPSEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2814..4133
                     /gene="potE"
                     /locus_tag="QBF46_003868"
     CDS             2814..4133
                     /gene="potE"
                     /locus_tag="QBF46_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDQ6675994.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            4209..4358
                     /locus_tag="QBF46_003869"
                     /pseudo
     CDS             4209..4358
                     /locus_tag="QBF46_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            4482..5132
                     /locus_tag="QBF46_003870"
     CDS             4482..5132
                     /locus_tag="QBF46_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HDQ6675995.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKDLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(5173..5667)
                     /locus_tag="QBF46_003871"
     CDS             complement(5173..5667)
                     /locus_tag="QBF46_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6675996.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     GLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(5881..7521)
                     /gene="pgm"
                     /locus_tag="QBF46_003872"
     CDS             complement(5881..7521)
                     /gene="pgm"
                     /locus_tag="QBF46_003872"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDQ6675997.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(7547..8092)
                     /gene="seqA"
                     /locus_tag="QBF46_003873"
     CDS             complement(7547..8092)
                     /gene="seqA"
                     /locus_tag="QBF46_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDQ6675998.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            8277..9041
                     /gene="ybfF"
                     /locus_tag="QBF46_003874"
     CDS             8277..9041
                     /gene="ybfF"
                     /locus_tag="QBF46_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDQ6675999.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            9181..9474
                     /gene="ybfE"
                     /locus_tag="QBF46_003875"
     CDS             9181..9474
                     /gene="ybfE"
                     /locus_tag="QBF46_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDQ6676000.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            9614..10144
                     /gene="fldA"
                     /locus_tag="QBF46_003876"
     CDS             9614..10144
                     /gene="fldA"
                     /locus_tag="QBF46_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDQ6676001.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            10354..10440
                     /gene="uof"
                     /locus_tag="QBF46_003877"
     CDS             10354..10440
                     /gene="uof"
                     /locus_tag="QBF46_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDQ6676002.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            10433..10879
                     /gene="fur"
                     /locus_tag="QBF46_003878"
     CDS             10433..10879
                     /gene="fur"
                     /locus_tag="QBF46_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDQ6676003.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(10964..11290)
                     /locus_tag="QBF46_003879"
     CDS             complement(10964..11290)
                     /locus_tag="QBF46_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676004.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(11340..12746)
                     /gene="chiP"
                     /locus_tag="QBF46_003880"
     CDS             complement(11340..12746)
                     /gene="chiP"
                     /locus_tag="QBF46_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDQ6676005.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         3217 a         3129 c         3196 g         3438 t
ORIGIN      
        1 gcaggtttaa tttcttaacg caaatataaa acacgaaatt acaacattta agtaacaaaa
       61 cagcatctaa tatggcgata ttcataatta atgatttatt atctggggta tgatcgtttt
      121 ttgttgatct tcttcacaga ttatagccat ttcatggata gaataactct accttcaact
      181 gacacagcaa gaggtaaagg taaatggaaa ataataaccg cttaatgcct catataaggc
      241 ggacaaccca tatcatgaag tttgcccatc gtaatagttt tgactttcat ttctttaatg
      301 cccgctagtc ttctgactaa agggcacccc aacgtacagg tctccctgac tttaagcatt
      361 acaggttaat acctgtattc ctcggtgctc atatactgct aaccctttta aactctaaat
      421 aattcgagtc gcagcacttg caacttgagg tatgacgagt atagccagtt accgggctgg
      481 tctgggttat tgcatctgca aaaagcaaac tactgattta tttatcagcg gtggagcttt
      541 gctttttttc cggcgtgatc gatttctcct ttgagaaatt gaggacctgc tattacctga
      601 aataaagaga tgaacaaaat gtcagaatta aaaattgccg ttagtcgttc ttgcccggat
      661 tgtttttcca ctcatcgtac atgcgtgaat attgacgaaa gtaattatat tgacgttgcc
      721 gccattattt tatcggtcaa tgatgtggaa cgtggaaaac tcgatgaaat agacgctact
      781 ggctatggca ttcctgtttt tattgcaacg gaaaatgaag aacgtatacc agcagaatat
      841 ctttcacgta tttctggtgt cttcgaacct agcgaagcac gtaaagaatt ttatggtcgc
      901 cagttagaaa ccgctgccag ccattatgaa actcaactgc gcccaccttt cttccgcgca
      961 ctggtcgatt atgtcaatca aggtaacagc gcgtttgatt gccctggtca tcagggcggc
     1021 gaatttttcc gtcgccatcc ggcggggaat cagtttgtgg aatactttgg tgaggcgctg
     1081 ttccgtgccg acttgtgcaa cgccgacgta gcgatgggcg atctgctgat tcacgaaggc
     1141 gcgccatgca ttgcacagca acatgcggca aaagtgttta atgccgataa aacctacttc
     1201 gttttaaatg gcacttcatc ttctaacaaa gtggttttaa acgccctgct aacaccgggt
     1261 gatctggtgc tgtttgaccg caataaccac aaatctaacc accacggagc gttgctacag
     1321 gctggtgcaa caccggttta tctggaaacg gcacgtaacc cgtatggctt tatcggtggc
     1381 attgatgcgc actgttttga agaaagttac ctgcgtgagc tgatcgcgga agtcgcaccg
     1441 cagcgggcaa aagaggctcg tcctttccgc ctcgctgtga ttcagttagg cacctacgac
     1501 ggtacgattt ataacgcccg ccaggtggtg gataaaattg gtcatctgtg tgactacatc
     1561 ctgtttgact cagcatgggt cggctatgaa cagtttattc cgatgatggc ggactgttcg
     1621 ccgctgttgc tggatcttaa tgagaacgat ccgggtattc tggttacgca atctgtgcat
     1681 aaacaacagg ctggtttttc tcagacttca caaattcata aaaaagacag ccacatcaaa
     1741 gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat gcacgcctcc
     1801 accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat gcatgaaggt
     1861 gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc ccgcaaactg
     1921 atcctcgata actgtcagca tattcgtccg ttcgtacctg aactggtgga tggtaaaccc
     1981 tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca gtttgtacca
     2041 ggggaacact ggcattcttt tgaaggctat gcagagaatc aatactttgt cgatccttgc
     2101 aaactgttgc tgacaacccc aggtattgat gcacgtaacg gcgaatatga agcgttcggt
     2161 gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc
     2221 gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa gcttcagcaa
     2281 cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa
     2341 gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac cctgcgtcag
     2401 ttgtgtcaag aaatgcatga tttgtatgcc cgtcacaacg tgaaacaact gcaaaaagag
     2461 atgttccgta aggaacactt cccacgcgta agcatgaatc cgcaagaagc caactacgcc
     2521 tatttacgcg gtgaagtgga gctggttcgt ctgccggatg cagaaggccg tatcgctgcc
     2581 gaaggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga aatctggggt
     2641 ggtgctgttt tgcgttactt cagcgctctg gaagaaggga tcaacctgct gccaggtttt
     2701 gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc
     2761 tatgtcatca agcctcgtga tgcgcaaagc accctgttga aaggggaaaa attatgagtc
     2821 aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat actgacgatg gtcaacatga
     2881 tgggctccgg tatcatcatg ctgccgacaa agcttgccga agttgggaca atttcaatta
     2941 tctcctggct ggtgacagcc gtaggctcaa tggcactggc atgggcattc gcaaaatgcg
     3001 gtatgttcag ccgtaaatca ggtggtatgg gcggttatgc cgagtatgct ttcggtaaat
     3061 ccggtaactt tatggcgaac tatacctacg gcgtctcact gctgattgct aacgtcgcga
     3121 ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg tcgccagtgc
     3181 agattggtct tgcgaccatc ggggtgctgt ggatttgtac cgtggctaac tttggtggtg
     3241 cgcgcattac cgggcaaatc agtagcatta ccgtgtgggg ggtcattatt ccggtggtag
     3301 gcctgtgcat tatcggctgg ttctggttta gcccgacgct ttacgttgat tcctggaatc
     3361 cgcatcatgc accgttcttc agtgcagtag gttcttccat cgctatgacg ctgtgggctt
     3421 ttctcggtct ggagtctgcg tgtgcgaata ctgatgtagt ggaaaacccg gaacgtaatg
     3481 tgccaatcgc ggtactcggc ggtacgttag gtgcggcggt gatttatatc gtctccacca
     3541 acgtgattgc agggattgtg cctaatatgg agctggcaaa ttcaacggca ccatttggcc
     3601 tggccttcgc acagatgttc acaccagaag tgggtaaagt cattatggcg ctgatggtga
     3661 tgtcctgctg cggttcgctg cttggctggc agttcaccat tgcccaggtg tttaaatctt
     3721 catctgatga aggctacttc cctaaaattt tctcccgtgt aaccaaagtg gatgcaccgg
     3781 tgcagggaat gttgaccatt gtgattatcc agagcgggtt ggcgctgatg acgattagcc
     3841 cgtcgctaaa cagtcagttc aacgtgctgg ttaacctggc cgtggtgacc aatatcattc
     3901 cgtatattct gtcaatggcg gcattagtca ttattcagaa ggtcgctaat gtgcccccct
     3961 caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc gatgtatagc ttttatgcgc
     4021 tgtactcatc cggggaagaa gccatgctgt acggttccat cgtgaccttc cttggttgga
     4081 cactgtatgg tctggtatca ccacgctttg aactgaaaaa taaacacggt taataaaaca
     4141 aagtaaaaaa gggcgattga atgatcgccc tttctgtttg ttggcaaata gttcgttatt
     4201 tatgccgcat ccggcagatg tagggtctaa acgcgatcga acacagcaac tatttgcgta
     4261 attatatggg gcatctgcga caaaaactgg aacaggatcc ggcccggcct cgccatttta
     4321 ttactgaaac cggtattggg tatcggttta tgctttgaaa aaagtggcaa tgtatggata
     4381 aagtcatttt ttattcaaat ataaggaact taatatttaa aaaatgttcc atacaattcc
     4441 tcatcataaa tgacaccgta gcacacaagg agcaagtagc gatgcgtgaa atgaatcagt
     4501 tagtaaatac tgaagattca gcctggccaa taatccagaa ctggttaaaa gacgccacaa
     4561 accataccga actcttaccg gttaataaag atttagccga aaccgcactt tatcaattac
     4621 aggtgacaac aaaatcacca atgggtgcgc ttgtctatgg ctccggaggt ctgctgattg
     4681 ataatggttg gttacgcata gcaggatctg gtcacccccg gttgccacga gatccagtaa
     4741 gttggacaca acgccctgaa ttcgcaggcg tgcgggcttt acccatcgct gacgatgtcg
     4801 cagggggaat ttttgctctt aatggtggcg atcttgggga agatactggc tgcgtttatt
     4861 attttgcgcc agatacgctg aattgggaaa gtctggaagt tggatattct gagtttcttc
     4921 agtgggcatt atccggtgat ttagatacct tctatgaaaa tgtacgatgg cagcaatggc
     4981 gagaagacgt aataaaatta tcggcaacgg aagccttcac gttctaccct ttcttatggg
     5041 tacaaagtga agaagcaaga acaagaaaag ttatttcgct tactgaactc tgggaaatgc
     5101 agtatcaaat gaaagaaaca ttcacgcaat aagacatgtt caggtccggg tttcatttcc
     5161 ggactcacta ttttaatgtg tatttatcgc tttcaattct tgctggcaat tttctgactg
     5221 gcagcgtcgt aactcaataa tcccgacatg catcatcgag ccaaaaaatc cgctttcgct
     5281 gacataatcc tcattgacta tcggtcgagt gccgggcttg ccctggcaaa cccgcataaa
     5341 aaatgaggtg tagcattgcc aaccgggatt taaaccgacc ttttcttgtg ctgacatatt
     5401 atgaagataa gtaagttctt tttcgttccc gcctgacaac tgccagcggc gggtattatc
     5461 aggtaagtta tcaccacggc aatttgtctt acaccgggaa ggtgcaatat ttaccgcaat
     5521 gctacccgtt aatcctctgg ttttatcttt aggggtcagt aaccagtcgc cagctaatga
     5581 tgattgttca ggttgggcgc atgcagtcag cagtaatgtg gcggtaatta gtgacactat
     5641 gacgaccaga ctcctgttgg tcttcattgg gatttccttg agctactcag tttgttgaca
     5701 aagtgttcgt tgtttttgcc ggatgcggcg tgaacgcctt atccggccta caaaatcgct
     5761 taaattcaat atattgcacc atcctcgtag gcctgataag cgtagcgcat caggcaattc
     5821 tgtgtttgtc atacgtaaaa aagggcgatc ttgcgaccgc ccttttttta ttaaatgtgt
     5881 ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct tctttctcaa tctgcttgcg
     5941 atgttcttca ccgaggaagc tttcgcagta gatcttatat gcgtcttccg tgcctgacgg
     6001 acgcgcggcg aaccagccgt tgtcagtcat cactttcaga ccgccgatag aagcaccgtt
     6061 gcccggtgcc gccgtcaggc gcgcggtgat cgggtcgcct gccagggtgc tggcgctcac
     6121 catttccgga gacagcttag acaacgccgc tttttgtgcg gaagtcgcgg ctgcctgcaa
     6181 acggttgtag ctcggcgcac caaagcgttt tgccagttcg ttgtagtgtt cctgcgggtt
     6241 cttaccggtg acagcggtga tttccgccgc cagcagacac ataatgatgc cgtctttgtc
     6301 ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc cctgcgctct cttcgccgcc
     6361 aaagccgaag ctgccgtcga acagaccatc aacaaaccat ttgaaaccta ccggtacttc
     6421 taccagctta cggcccaaat cgttgaccac acggtcgatc atcgcagatg aaaccagcgt
     6481 tttacctaca gcaacatctt tgccccactg cggacgatgc tggaacaggt agttgattgc
     6541 caccgccagg tagtggttcg gattcatcaa acctgccgga gtgacgatac cgtgacggtc
     6601 ataatccggg tcgttagcaa acgccagatc gaacttatca cgcagtgcca gcaggcccgc
     6661 catcgcacac tcggaggagc agtccatacg gatcgcgccg tctttatcaa ggtgcataaa
     6721 gcggaaggtt tgatcgacct gatcgttaac gatagtcagg ttgaggttgt aatactcgcc
     6781 aatacgcttc cagtattcga taccggaacc gcccagcgga tcaacgccaa gcgtcaggcc
     6841 cgctttctga atcgccgcca tatcaacgat atcggccagc ccttccacga acggctgcac
     6901 cagatcctgc tctttcacat gaccggatgc catcgcttcg tcgagggaga tacgcttcac
     6961 gcctttcagg ccatcggcca tcagtgcgtt ggccctgtct tccaccactt tggtgacgtt
     7021 ggtatcagcc gggccaccat ttggcggatt gtatttgatt ccaccatctt ccggcgggtt
     7081 atgggacggt gtaatcacga taccgtctgc cagcgggcca ccttttttat tgtgaaccag
     7141 gatggcattg gaaacggcag gcgtcggagt gaatccattg ttttcctgca caatgacatc
     7201 aacgccgttc gctgccagca cttccagtac ggaaatgaac gcaggctcgg acagggcgtg
     7261 agtatcttta cccacatagc aagggccagt gatgccgttt ttcgcacgtt cttcagcaat
     7321 tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg tggcgcgctg cactgccacg
     7381 gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc cctgcttctg gtttcagtac
     7441 ataatattgc gccgtcagtt gggcgacgtt aatcaaatca ctctgttgtg caggttggcc
     7501 tgcacgattg tggattgcca ttgctttgtc ctttgtctgc aacgttttag atagttccgc
     7561 aaaccttctc aatcaattcc gccgggaatt gcatcgactg catgatgtgt tcgatcatgc
     7621 tgcatttacg gccggtgttg gtgttggtga tcacccaata cggcgtgcct ggcacatgtt
     7681 tcggcttggt ctgattacca tttttcagca gcgtttgttc atctgccgca aagtaaacgc
     7741 gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc ctgggcgtca agagaatata
     7801 gtgtagacaa cagcagcata aagcgattga ccgctcgctt ttgctctgcg tattcgtccg
     7861 aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat cgttttgacc ggcttcgctt
     7921 cgacgatagc aggtgacgca acgcgaacct ctttcgtcac cggagcagca ggctgtgatg
     7981 cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc gctctcgccg atatgcttag
     8041 tgtggctggc aatatagctg tagagttcat catcaacttc aatcgttttc atcttaatcc
     8101 agtgcagtgt cttagctgaa tacaagtcgc caggagtata tgaataaatc ccggcagcgg
     8161 atagcgtcaa gcctggcttg cagaaaaact cggaataaac cttatttgtt gcagccctgt
     8221 ggcacaatgg tcaacataat accctaaccc gacaacgcga aaaaaagaac tttgccatga
     8281 aattgaatat ccgcgcgcaa actgcacaaa accagcacaa taattctccc atcgttcttg
     8341 tccatggtct gtttggtagc ctcgacaacc ttggcgtact ggctcgcgat ctggtaaacg
     8401 atcacaatat catccaggtt gatatgcgta accacggtct ttcaccgaga gagccggtaa
     8461 tgaattaccc ggcgatggcg caggatcttg ttgatactct ggatgcacag cagatcgaca
     8521 aagcaacatt tatcggtcac tccatgggcg gtaaagcggt aatggcactt acggcactgg
     8581 cccgcgatcg catcgataaa ctggtggcga tcgatatcgc gccggtcgac tatcacgtac
     8641 gccgtcatga tgagattttt gcggccatca acgcagtcag tgaatcggac gcacaaactc
     8701 gccagcaagc agcagcaata atgcgccagc atcttaatga agaaggggtg attcagtttc
     8761 tgctgaaatc ttttgttgac ggggagtggc gctttaacgt gccggtattg tgggatcagt
     8821 atccgcatat tgtggggtgg gagaaaatcc cggcatggga tcaccctgcc ctgtttatcc
     8881 ctggcggcaa ttctccgtat gttagcgagc agtaccgtga tgatttactg gcacaatttc
     8941 cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg ggtccatgct gaaaaaccgg
     9001 atgcggtatt acgcgctatc cgtcgctatc tcaatgatta acgcaactga ttaaaaaccc
     9061 agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac tcatcgcgct gatgtattat
     9121 ggcgcgctat ccatccgggc agaggcctgg ctgattgttt cccccgaagt caccaagatc
     9181 atggccaaag aacaaacgga ccgtacgaca ttagatctgt tcgcgcacga gcgtcgaccg
     9241 ggacgaccga aaactaatcc gctttcgcgc gatgaacagc tgcgtattaa taaacgcaac
     9301 cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg aactgaagct gaacgcggaa
     9361 gctgtcgagg cgctgaacga gctggcggaa tcgcgcaata tgagccgtag cgaactgatc
     9421 gaagagatgt taatgcagca actggcggcg ctgcgtagtc agggcatcgt ttaaatttcc
     9481 actttcatgt agcacagtgt gcagtcctgc tcgtttgctg attccgcaag actgcctgtt
     9541 ctgctatgat tgcctttatc cgtgggcaat tttccacccc catttcaata agtttcaaga
     9601 ggttatttca ctcatggcta tcactggcat ctttttcggc agcgacaccg gtaataccga
     9661 aaatatcgca aaaatgattc aaaaacagct tggtaaagac gttgccgatg tccatgacat
     9721 tgcaaaaagc agcaaagaag atctggaagc ttatgacatt ctgctgctgg gcatcccaac
     9781 ctggtattat ggcgaagcgc agtgtgactg ggatgacttc ttcccgactc tcgaagagat
     9841 tgatttcaat ggcaaactgg ttgcgctgtt tggttgtggt gaccaggaag attacgccga
     9901 atatttctgc gatgcattgg gcaccatccg cgacatcatt gaaccgcgcg gtgcaacgat
     9961 cgttggtcac tggccaactg cgggctatca tttcgaagca tcaaaaggtc tggctgatga
    10021 cgaccacttt gtcggtctgg ctatcgacga agaccgtcag ccggaactga ccgctgaacg
    10081 tgtagaaaaa tgggttaaac agatttctga agagttgcat ctcgacgaaa ttctcaatgc
    10141 ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta atagataatg ccaatcaaaa
    10201 taattgctac aaatttgtaa cttttgctgt tgtacctgta caatgtcccg gtgttcaagt
    10261 ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat taacaatatt tgccagggac
    10321 ttgtggtttt catttaggcg tggcaattct ataatgatac gcattatctc aagagcaaat
    10381 tctgtcactt cttctaatga agtgaaccgc ttagtaacag gacagattcc gcatgactga
    10441 taacaatacc gccctaaaga aagctggcct gaaagtaacg cttcctcgtt taaaaatcct
    10501 ggaagttctt caggagccgg acaaccatca cgtcagtgcg gaagatttat acaaacgtct
    10561 gatcgatatg ggtgaagaaa ttggtctggc tacggtatat cgcgtactga accagtttga
    10621 cgacgctggt atcgtcaccc gccacaattt tgaaggcggt aaatccgtat ttgaactgac
    10681 acagcaacat caccacgatc acctgatctg cctcgactgc ggcaaggtta tcgaatttag
    10741 tgatgattcc atcgaagcgc gtcagcgtga aattgccgca aaacatggca ttcgcctgac
    10801 taaccacagt ctctatcttt acggtcactg tgccgaaggc gattgccgcg aagatgaaca
    10861 tgcgcacgaa ggcaaataag ccagcctgaa tgagaaaaag ccaacctgcg ggttggcttt
    10921 tttatgcaag ggaaataaga aaagcgagag ttacagctct cacttatttg ttattactgc
    10981 gaatttcctg ccaaacttta tcacaatcgg ctttcaccgc ctgatcattt ccggtttgcg
    11041 ttgcacgcag acactgctga taatccagta cgcggacact ttcctggtcg gcaaaatgct
    11101 gatgttgttt ctctttcttc agcacgttta acacgctctg gcaggcttca attttttctg
    11161 gcgaaccttg tgcggtgttg atacaggcgc tgtatgcctc tttcagacga ctgtcttcct
    11221 gtggcgcggt tgattgcgca caagctacca gccccgatgc cattatggcg atgagaatca
    11281 gttttttcat tgaaaattcc tcaaacgtgc ggcaggcaca aaacctgccg cgtcgtgcat
    11341 cagaagatgg tgaatggtgc gattaccata aatttcacgt cacgctcatc ctggaagatg
    11401 ttaccgtaac cgccgcccca gcttgggatg tcggagtggt tgtcgtattc ggtgaagtgc
    11461 agtttgaaca tcgtgccttt ggcgcgaccg tcctgaatgg tgtagaccgc atccaggctg
    11521 taggcagact cttcaatagt ccggtttttg tcgtagtacg catccggatt gctctgccag
    11581 gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa gcctggaaga
    11641 ttccagtttt tcaggtcata catcgcaccg aagaagaccg ctttttcgcc gttggcgttg
    11701 aagtcagaac ggttatccca ccagatatcc aggcgaccgt ttgaggaagc gtaggttgga
    11761 gtcatacgtt gcaggaagta tccctgctga ccgtcagcct taacccaggt gccttcgagg
    11821 cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca acgcttgcag ccaggcggtg
    11881 ccgtcataaa gatcgttgac gctgcgatcg tcaactttat cgcgggtacc gtagaactgg
    11941 tagctggtgg ttaacgggct accggcgata tcaaatttgt agctggcttt ggcaaaatat
    12001 tgatcgatat acccttccgc ctgaccaaat gctgcttcca gtacgaagtt atttttgaag
    12061 tcgtatttcg ccccaaggga gtgcagataa tcaactttgg tggttttatc gttctgataa
    12121 aactcatcca tttccagatg ccatggtgct ttgtattcat tggtccacat gtaggagaaa
    12181 ctcaacgcac cagcatcgcc gtaatcaaaa ttcgccccgg cttccgcccc ctgataagta
    12241 cctggcataa agctccagtg cggcgctaac agcgtttgac cagttggctg aatgtaacct
    12301 gccctcgccc aaaccggacc gtatttaaat ttggccgcag ctttatacag gcttataccg
    12361 cttttgtcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa cgcgatttcg
    12421 ttcgggtggg agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc agcaatatca
    12481 aggccgaaca tatcagcagc ataaccggac tgaaaatcga ggttggcatt ccaggtggag
    12541 tgagaaaggt tggttttgta tttgtcgccg tcggtaacat ctttacggtc gcgttcacgc
    12601 tgccagtaat atataccgcc ggttaaggtt gaatcgtcga tgaatccttc ggcgcgagcc
    12661 tccggcattg ccataaagcc cgacattgct gtaacaccgg cgatagccag cgccagcgta
    12721 ctacgtttgc cactaaacgt acgcatgggt taatcctctt tgacgtataa attgctgcac
    12781 caaaggtgaa cagcgaaaaa ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga
    12841 ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg
    12901 taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt aagtttgcgg
    12961 ttgaaaggct tgcagaagaa
//
LOCUS       DAOFQD010000132        12939 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.132, whole genome shotgun sequence.
ACCESSION   DAOFQD010000132 DAOFQD010000000
VERSION     DAOFQD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.132"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..130
                     /locus_tag="QBF46_003881"
     CDS             <1..130
                     /locus_tag="QBF46_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDQ6676006.1"
                     /translation="CLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(137..976)
                     /gene="ycjM"
                     /locus_tag="QBF46_003882"
                     /pseudo
     CDS             complement(137..976)
                     /gene="ycjM"
                     /locus_tag="QBF46_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(1189..1503)
                     /gene="pspE"
                     /locus_tag="QBF46_003883"
     CDS             complement(1189..1503)
                     /gene="pspE"
                     /locus_tag="QBF46_003883"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDQ6676007.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1578..1799)
                     /gene="pspD"
                     /locus_tag="QBF46_003884"
     CDS             complement(1578..1799)
                     /gene="pspD"
                     /locus_tag="QBF46_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDQ6676008.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1808..2167)
                     /gene="pspC"
                     /locus_tag="QBF46_003885"
     CDS             complement(1808..2167)
                     /gene="pspC"
                     /locus_tag="QBF46_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDQ6676009.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2167..2391)
                     /gene="pspB"
                     /locus_tag="QBF46_003886"
     CDS             complement(2167..2391)
                     /gene="pspB"
                     /locus_tag="QBF46_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDQ6676010.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2445..3113)
                     /gene="pspA"
                     /locus_tag="QBF46_003887"
     CDS             complement(2445..3113)
                     /gene="pspA"
                     /locus_tag="QBF46_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDQ6676011.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3280..4257
                     /gene="pspF"
                     /locus_tag="QBF46_003888"
     CDS             3280..4257
                     /gene="pspF"
                     /locus_tag="QBF46_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDQ6676012.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4377..5642)
                     /gene="puuE"
                     /locus_tag="QBF46_003889"
     CDS             complement(4377..5642)
                     /gene="puuE"
                     /locus_tag="QBF46_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDQ6676013.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAVEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5680..6960)
                     /gene="puuB"
                     /locus_tag="QBF46_003890"
     CDS             complement(5680..6960)
                     /gene="puuB"
                     /locus_tag="QBF46_003890"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDQ6676014.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKNYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTVKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6962..8449)
                     /gene="puuC"
                     /locus_tag="QBF46_003891"
     CDS             complement(6962..8449)
                     /gene="puuC"
                     /locus_tag="QBF46_003891"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDQ6676015.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="QBF46_003892"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="QBF46_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDQ6676016.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="QBF46_003893"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="QBF46_003893"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDQ6676017.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="QBF46_003894"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="QBF46_003894"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDQ6676018.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="QBF46_003895"
     CDS             11851..12015
                     /locus_tag="QBF46_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676019.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..>12939
                     /gene="puuP"
                     /locus_tag="QBF46_003896"
     CDS             12005..>12939
                     /gene="puuP"
                     /locus_tag="QBF46_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDQ6676020.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASV"
BASE COUNT         2944 a         3444 c         3334 g         3217 t
ORIGIN      
        1 atgtcttgcc caatatactc aacatgaact agatctggtt gctgctcagc taaacaacag
       61 accgagaaag acactgaagt tcaaaacacc gaaagagata attgaaaggg gtgttgcatt
      121 gacagattga atctacaatg ttgtctttat gcggaacatt ggtctcggta atgatcactg
      181 tacctggcgc aacgttatca ataatcgacc gtaacagttt gataatcaga tgtgtttttt
      241 ccagatggat gcagcttgtt cccggctctt tccacataaa gccaacggca tccaggcgga
      301 catactcagc acctttcgca aggtaacaga gtaaaacatc caccatcgcc agcaacactt
      361 cagggctacg gtaattcagg tcaatttgat cgtcactaaa ggtggtccat aaatggcgcg
      421 ttgaatgatc gcgcatctgg aatggcgtta ataacggtaa cgcacgcggg cgagtgacgg
      481 cgctgagatc ggtttgcggg tcaacggcaa taaaaaaatc ttcaaaacct ggatgctgtt
      541 gtaaatagtt tttaaaccat tcactttttg ccgacatatg gttgcagaca aaatcaaaca
      601 ttaaatgact gcattcaccg agttgctgaa tatcctgcca ctcccccgct tcactggcga
      661 cctgatgata atcaattacc gaaaagccat catcagatga ccatggataa aacggcaaca
      721 aatgaacatg tgaaaaaatg ctttgcagcc attgatggta aaactgatta aatgtgggta
      781 atggttttaa atcattgctg tgaaattgat cggcataggt aattaacacg acatcacttt
      841 catcccagtg ttttttacgt cgctgtgtaa ttaatcgttt cgcactctca agacgcgtta
      901 caagtttctg taaatgagtt gcggtaaatg ttccaccgta gatttcgtcc aggtaatccg
      961 taattttctg tttcatgttg cctttccgtg gtagcggtcc catgaaacga gagactactc
     1021 cgcagaaaat aaactgtcaa ttgagcacaa gggttttagt cacgcaggaa atcatttttt
     1081 accagggaaa aagcgtattt gtgagctgac gcaaataatc tatggggatg taaataaggt
     1141 gtgacagaaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt aacctttgac
     1201 cttcggcatt gcgatgtctt tcaggccacc ggcgttctca acgtgggtat atcccatctc
     1261 gctaaggatc tcttttgctt gccctgactg gcgtccggca ttgcaataca ctttcacggt
     1321 gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca ggggaatatt
     1381 gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat cgatccagtg
     1441 ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa
     1501 catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga gatttaacaa
     1561 tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc agcccgactt
     1621 aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc aactgatttt
     1681 atcgcccagc ccgccacacc cgccgggcca tagcgcagtg cggtaagaag taccagcttg
     1741 cctgctaatt tgaaaccagg ctttaccttt tgcccggcct gttgccagcg agtattcata
     1801 actttcctca cagttgacgg aaacggctac gtaacgtgaa agtatcggaa gtgacataac
     1861 gttccatctc gcgtaaacgc gtttcacttg ccgccagttc acggtcgact tcatccagca
     1921 attcgctgct ggaaggtagc tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg
     1981 caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca
     2041 gcaccaccag gatacgcacc agttttaccg gtacatcaaa atagttggca atcccggcgc
     2101 agacgccgcg gaccatgccc tgctgtggaa tacgccataa ttttttattg agattaatgc
     2161 ccgccattag cgatccctcc agttcggatg ttctgcatca agaatagatt ccagcgcctg
     2221 aatacgttcg cgcatccgtt ttgcttcatc agccagttgc gctaatcgct gctgctcact
     2281 ttgcgacaat tcactgcgac cagaacgatt gctgtaatgc agccataacc agatcggtaa
     2341 aacaaacagc acaaaaatgg ttaacggaat agccagaaat agcgcgctca taagtactcc
     2401 ttagacgatg agcggcggcg cgttcggacg ccgcctggat gttattattg attgtcttgc
     2461 ttcattttgg cttttaattg tgccagttgt tcgctgattg catcatcggc tttcagttcg
     2521 gcaaactgat cgtccagcga tttttgttta ccgaagctgt ggctttctgc ttccgcttcc
     2581 atctggtcaa tacgacgttc gaaagattcg aaacgagcca ttgcttcatc cagtttgcca
     2641 ctgtccagct gacgacgcac atcgcgcgac gagtttgccg cctgatgacg taacatcaat
     2701 gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca gctcgccaat ctctttcttc
     2761 atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt ccagggactt aatcagatcg
     2821 gtcagtttct gtttttcaat taacgctgca cgtgccagat cctctctctc tttcagcagc
     2881 gccagttcgg ctttttcctg ccattcaact tcacgcgccg acgcttgttc aatacggcga
     2941 gtcagctgtt tcttttctgc caacgcgcgc gccgaagtag aacgtacttc aaccagtgta
     3001 tcttccatct cctggatcat cagacgaacc agtttctgtg gatcttccgc tttctctaac
     3061 agagcgttga tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg
     3121 tcctcttgat ttctgcgttc aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca
     3181 attttttatc tttttgattt ataaagatct gattgaagaa tcaacagcaa catgccagga
     3241 tgagttagcg aattacacta acaagtggcg aatttcatca tggcagaata caaagataat
     3301 ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg
     3361 ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagct gattgccagc
     3421 cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg
     3481 ttaaatgaaa atctgctgga ttccgaactg tttggtcacg aagcgggggc gtttaccggt
     3541 gcgcaaaaac gtcatccagg gagatttgaa cgtgccgacg gcggtacgct atttcttgat
     3601 gaactcgcta cggcaccgat gatggtgcag gagaaattat tgcgcgtgat tgagtacggt
     3661 gaactggagc gcgttggcgg cagccagcca ttgcaggtga atgtgcggtt ggtatgcgcg
     3721 acgaatgccg atctcccggc gatggtcaat gaaggcactt ttcgcgctga cctgctcgac
     3781 cgactggctt ttgatgttgt acaactgcca ccactgcgcg agcgcgaaag cgacataatg
     3841 ttgatggcag aacactttgc catccagatg tgtcgggaaa tcaagctgcc tctgttcccg
     3901 gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg aaatattcgt
     3961 gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga ttatccgctt
     4021 gatgacatca ttattgatcc ctttaaacgg cgtccgcctg aagacgctat cgccgtttca
     4081 gaaaccacct cgcttccaac actgccgctg gatttacgtg agtttcagat gcagcaggaa
     4141 aaagagttgc tgcaactcag tttgcaacaa gggaaatata accagaagcg cgcggctgaa
     4201 ttactggggt taacttatca tcagttccgc gcgttgttga aaaagcacca gatttagcga
     4261 agttgagcaa tacaacttgc cggatgcggc gtgaatgctt tattcggcat acataatcaa
     4321 tacgtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt gggctattaa
     4381 tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc cgggatggtc
     4441 agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag gagcagcccc
     4501 tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc gcccgtttgc
     4561 ggatcgttaa actctaccgc aatcatcgac cccaggccgc gtaccgcagc aatggccgga
     4621 acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg attcgcgcgt
     4681 tcgcagagtg attctttgtc gataatattg agcaccgcgt gcgcggcagc caccgccagc
     4741 gggttaccgg cgtaggtgcc gccaagcccg cccggcgcgg gtgcgtccat aatattcgcg
     4801 ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat cgtcattaaa
     4861 tccggcttat cggcgtaatg atccatggca aacagcttac cggtacgcgc aaagccgctt
     4921 tgcacttcat cagcaatcat cacaataccg tgctcgtcgc acaggcggcg aatagcggca
     4981 accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc gaaaataatc
     5041 gccgccacct gcttcgcttc gatgtctgat ttaaacaagc gttcgatggc gtcgagggag
     5101 tcctgtgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac cgaaccaggg
     5161 aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat ggtcatatac
     5221 gtacgaccgt gaaagccgcc gctaaacgca atcacgccag ggcgtccggt atgggcgcga
     5281 gcaattttca ccgcgttttc caccgcttcc gcaccggtgg tgaagaacgc ggttttggcc
     5341 tgcccgctca ccggggcaag ggcgttgatt ttctccgcca gggtgacgta gctttcatac
     5401 ggcacaatct gatacgcggt gtgggtaaac tgttgcagtt gctgctccac cgccgcgacc
     5461 agatcagggt ggcgatgtcc ggtattcagc accgcaatgc ctgcggcgaa atcgatgtac
     5521 tcgttgccct caacatcctt cagcgtggcg ttttcagccg actgggcgaa gaagttacac
     5581 atcacgccaa ccccgcgcgg ggtggcagaa agacgacgct gatggaattc attgttgctc
     5641 atgtttcaca ccttttgact cttcacggtt aatcggaaat taaacgccca gacgatcgcg
     5701 caggctgtaa tacgccgcgc ccatcgcggt aaacggcaca cgcagcgtgc gcccgccggg
     5761 gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgt tcggcgtcgc cgcgcagcag
     5821 ttcggcaatc aaacgtccgg ctagatgagt acaggtcacg ccgtggccgc tgtagccctg
     5881 catgtaatag atgttggtat cgaggcgacc aaactgcggc atacgcgaca gggtcagcag
     5941 gaagttgccc gtccagcggt aatcaatttt cacgcccttc agctgcggga aggttttcag
     6001 cagtttcggc accacaaggc gctcaacgtc atccgggtcg cgcgcgccgt agaccacgcc
     6061 gccgccgtac agcaggcggt tgtcggcggt aagacggtag taatcaagca gatagttaca
     6121 gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg acagccgttc
     6181 ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gccagttccg gctctacttt
     6241 atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct gacctttgac
     6301 agttcgcaca acggctggcg tggtgtgctg gatctgcgtc acggcagaaa gttcatacac
     6361 gcgcccgccg ttgaggcgga tggcgtccgc ttcgccaatg gcgaggttta gcggatgaat
     6421 atgcccaccg ctgtgatcca gcagcgcacc agtgtagcga tcgctggcga cctcgcggcg
     6481 aatagcgttg gcgtccagca gctccagctg tttattgccg tagcgttccc agttctcttt
     6541 ctgctcttcg agtgtggcga gttgtttatc attcatcgcc acaaacagac cgccgggacg
     6601 atagtcgcag tcaatctgat aacgtttgat acgttcgcgg atgatctcgc caccctcgaa
     6661 catcatgctg ccgagcatac gggcggtgtc catgccgtag tttttttcga tcacgtcgat
     6721 gtcgcggctg taggagttca caagctgccc gccattgcgt ccgcttgcgc cgaagccgat
     6781 gcgtgaggct tcaagaacca ccacgtcaaa gcccgcttcc gccaaatgca gcgcggaaga
     6841 gagcccggta tagccgccgc cgaccacgca aacgtcgcag gaaatcgact cattcagcgt
     6901 gtcgaatggc gcgtatttat tcgcactggc ggcgtagtaa ctgctggtat gttcggtcat
     6961 ttcaggcctc caggcttatc cagatggttt tcagttcggt gaatttttca agggcatgca
     7021 gggatttgtc gcgaccgttg ccgctctgct tatagccgcc aaacggcacg gtcatatcgc
     7081 cgtcgttgta gttattgacg aagacggaac cagccttcag gcggcggctc atgcggtgcg
     7141 cgcgggagag gtcgcgcgtc cataccgccg cgccaaggcc gtactggctg tcgttggcaa
     7201 gctgtagcgc ctgttcttct gatgtgaaac gcgtgaccac cagcaccgga ccgaaaatct
     7261 cttcgcggct taaggacgca ttcgggtcta catccacaaa gatggtcggg ccgatggcgg
     7321 cagccagctc agcgttacga ccatccagca gcagttgccc tttgctttcg ccttcccgaa
     7381 taaagctatg gaccgagtcg gcgtgggcgc agtcgattaa ggtgcccatg gtggttgcgg
     7441 gatcaagtgg atggcccggc tgccagtttt gcgcctgctg ttttaacagg gctaagaatt
     7501 catcggcgat gctctcttcc agcaacaaac gcgttccggc gatgcacacc tggccctggt
     7561 tgtagaagat gcctgcggcg gtggcgcttg ccgctttttg cagatccgga caatcggcga
     7621 aaacgatgtt ggcgcttttg ccgcccgctt ccagccagac gcgtttcatg ttgctgtcgc
     7681 ccgcatcttt cagcagctgt ttcccggtac gggttgaacc ggtaaaggca atggcgtcga
     7741 tatcgttatg acgcgacagc gcctgccccg cttcatgacc aaaacccgtc accacgttca
     7801 acacaccatc cggcaagcct gcttctttcg ccagccccgc gagacgaatc gcactgagcg
     7861 gtgatttttc agacggtttc aggacaacgc tgttccctgc tgccagcgcc gggccaagct
     7921 tccagcaggt cagcagcagc gggaagttcc acggcacgat ggcggcaatc acgccgaccg
     7981 gttcacgcac gatcatcgcc agctcatggc tactggtggt cgccacttcg ccatacactt
     8041 tgtcgatcgc ttcggcgtac cagcgaatgg cgcgcgccgc gccgggaata tcatcacgca
     8101 gactgtgacg aatcggtttg ccggtgtcga gagtttccag cagtgccagc tcttcggcgt
     8161 gggcttccat taaatcggcg agtttattca gtaccgcttt acgtttagcc ggagaagaga
     8221 gtgaccagtc gccgcgttca aatacgccgc gtgctgcgct caccgcacgg tcgatatcga
     8281 cgctcttgcc gcgggcaatt ttcgccagcg gtgcctgggt gaccggatca acggtttcaa
     8341 aggtttcatt ttccgccgca gcagtatatt caccgttaat aaataagcgg ttttcaatgg
     8401 cgagacttaa cgctttatcc tgccagtaag ccagatgatg aaaattcatt atgactcctg
     8461 tttcacgtct atcagatata tgcagtttgt gaacgtcagg gcgcagagat ccccgggccg
     8521 tatgcccgtt tatttctggc gacattcttt atatacactt catccttcaa gccgcctctg
     8581 ccttggctgc gttcgctccc ccgaatcact tacctgagta agctcatcgg gattcgctca
     8641 cttgccgcct tgatgcaact tgaatgattt tgtgtataga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg acaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactaccag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtggg ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttagtttgca tacagattcg aatggtggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agttggagaa agggtgttat ttcccggcct
    10501 cggtgtttgc aatggatatt ctgggcaacg tagtagaaga agcgggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtact tgtgttcctg ttctcggttc cttaacccct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggtcgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 ggtatctgca accgccctgc gcgcccggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaat cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttacagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcttgcga tgatgcgctg gcactaaaac
    11041 gcctggtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc ggaatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggcgacc gtcataatca ccgcgtggaa tatcgcgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattccccat gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgcgact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 tgtgttcgat acttttggca ttgtgtcagg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggtg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcatgtcgg
    12301 ctttatggtc ggctggtcat cgctgctgga ttatctcttt ttgccgatga ttaacgtctt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaattt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtagt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggggcgacg attgtctgtt tctccttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtctacgg tggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagat ccggatgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt ccagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacgtta gcgtctggcc tggcttcaca tgccagcgt
//
LOCUS       DAOFQD010000133        12739 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.133, whole genome shotgun sequence.
ACCESSION   DAOFQD010000133 DAOFQD010000000
VERSION     DAOFQD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.133"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..289)
                     /locus_tag="QBF46_003897"
     CDS             complement(<1..289)
                     /locus_tag="QBF46_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676021.1"
                     /translation="MTTSDVIALGALAVSIYTIISSEIRSRKNEREQKVIKDEQDRLR
                     KLLLEKETKSAINDMKAELGARLVKIARNNYRLKVFNRGKVEARNIEIHFPD"
     gene            complement(289..564)
                     /locus_tag="QBF46_003898"
     CDS             complement(289..564)
                     /locus_tag="QBF46_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676022.1"
                     /translation="MKLNPEKYNRKITLLCPVCGGSEMEHTEELEDVKCVGCGKEFTN
                     DELIQENGVSIDAHVNEVKEELSKDIQKQFNDILKKAFKGSKNIRIK"
     gene            complement(1072..1779)
                     /locus_tag="QBF46_003899"
     CDS             complement(1072..1779)
                     /locus_tag="QBF46_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDQ6676023.1"
                     /translation="MARCTAPVRGHSSSAAAAACPACRNRGSYSRGSYGYDSYSSYPS
                     SGNSGGGSYGRSGGGSSSSTKPRWSKSSSSVSYTTAQVQSLTPIRQVVEKLAAEQPDL
                     RDVFLCHAWDDRQGIAKELHDLLEAAGVKVWFSEKDLALGVPMMRAIDKGLANSRIGL
                     VLVTPALLSRLPKEGVADKELSALLAGNQLVPIVHNTTYEALRNVSPLLASRSGLDTG
                     EDTLAVVATKIAELITV"
     gene            2158..2691
                     /locus_tag="QBF46_003900"
     CDS             2158..2691
                     /locus_tag="QBF46_003900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676024.1"
                     /translation="MPGTKCVTVLTARGITEILKTGGSKAWRLNATHAGKCSYVVCVQ
                     NSKIDWGTQEAKHHHAFMIGRISGISRIPDHPKKRWIINIDSYAEIDIPDQWDGNRNP
                     VSYRNLEDMGIDLDTLDFKPVGHIVSGIKQTSGDEYDEYEEVDVSDRPLSLKEAKAGL
                     ALHFGVNEDDIQITIKG"
     gene            2697..3281
                     /locus_tag="QBF46_003901"
     CDS             2697..3281
                     /locus_tag="QBF46_003901"
                     /inference="COORDINATES: protein motif:HMM:NF019843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDQ6676025.1"
                     /translation="MESFSVEHWLPQATAGDSQARFYLGKIFSCGLGVHQDYKLAIYW
                     FQLAAEQGHDDAQFNLGWVYHLGLGGYKHVQKASEWYQKAALQGHAEAQFHLGRLYAF
                     SDLRNDGLAVEWFLCAAKQGYSSAQFKLGWMYHLGMGVKQSDRQAFHWFIRAAEQDHA
                     GAQASLGEMYKHGWGVEQDETMAQYWFRLARTMP"
     gene            3596..4834
                     /locus_tag="QBF46_003902"
     CDS             3596..4834
                     /locus_tag="QBF46_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004132572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDQ6676026.1"
                     /translation="MTATNQFAVHVGLDWADKKHDVCVQFKNGERVFHVIEHTAEALD
                     AWLTELHQKVKGRIAIALELKKGPVVYALQKYPFITVFPVHALSLARYRQAFSPSGAK
                     DDPQDAELALELMLRYPQKIKAIEPDNADIRLLQQLVEQRRQLVEDKRRFVNRLINTL
                     KQYYPQPLEWFSHRGSLLLCELIMRWPSLQQLKRARHDTIRNFLNAKGGRAMALTEQR
                     VTNIDNAIPLTTDPSVIEANALMATALATQIKVVSEIIKTYDERIETLFDTLPDAGLF
                     KSLPGMGPCMGPRMLAALGDNRDRFNSAEEIQNYAGIAPVTERSGQKSWVHWRWQCAK
                     FVRQTFVEWAAKTVNSSYWARLYYQGQRDKGKSHQSAIRALAFKWIRIIYRCWKTRTR
                     YDEAKYLLALEARNSPLLRP"
     gene            complement(4897..6051)
                     /locus_tag="QBF46_003903"
     CDS             complement(4897..6051)
                     /locus_tag="QBF46_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDQ6676027.1"
                     /translation="MPNEFWIAGAGSGKTQKVIEDAIEVIKAGGRVLVVTYTMNNQAE
                     LRSRFVELYGKHSDDFVVKGLFSFYLEDLVRPYQTAIFPDRITSTMFTEHNPHLIPGT
                     RKWHPKRGEKINGALNPLHYLTPCKTKAYTGLLAKLATRISASTKNAPANRLKEIYQR
                     IFFDEVQDLVGWDFDVIKALSKAMRNSICCVGDFRQTIYTTTFGHKAPETPEQKIQYF
                     KDLNFSPFSLAKNRRCIQEICDLADTIHPGIYEQTQSEVGEVPAEMAHHCGCFLIKPS
                     QVDDYLDIFKPQVLRWSSPMGKGYLPPGLTCHTFGSCKGLGFDRVLIIPPEKHLKYLG
                     GDLGVFDKDKTEESRNKLYVAITRARYSLAFVVEEEHLTNIKLPHWARPL"
     gene            complement(6044..7702)
                     /locus_tag="QBF46_003904"
     CDS             complement(6044..7702)
                     /locus_tag="QBF46_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6676028.1"
                     /translation="MRVKSIKLTNFKKFKDEHFEFNDDVNIFVGDNNAGKSTILEALE
                     IVLNYSYRGRPFNSEFTPDIFNKDAVQLFLASDKSATHLPVLAIEAFIEGVPEYRGTN
                     NFLKADAQGITVLVRFDDTLKDVYADHLLTNPHITSIPIEFYKLEWLDFGWNPVKAVA
                     KKFRALYIDPTRIHPTLGKNQYISTILNTALKKEELVKLTLNYRENQQVFNNSGEVRT
                     VNTGLDTDHLITEKKLSIAASTLPAGSIQTSLQLEVDDVPFQFIGKGEQSNVQIKLAI
                     QNKSKDIDLVMMEEPENHLSHTNLNKLVHYIENQRGDKQLFLTTHSSYVLNKLSIDKI
                     CLVQSGYKRLHKLAPAVVKTLKRLPGYDTLRVALSHKVILVEGPSDELVLKKIYLRKH
                     NRLPEQDGIDIIVVRGVGFDTFISVGMEIGTRINVLRDNDGDYDENVVKVRADYAAYP
                     NIKLISSAKNEEFSLEPAMIYANGTDLVTLDAFAKVVLSTQTFNLYDKVVDLDKRRDF
                     LIDWFRSVQGNGKGARKVDSAIKLFDGTLNFRYPPFLDEVFDFA"
     gene            8001..8240
                     /gene="relB"
                     /locus_tag="QBF46_003905"
     CDS             8001..8240
                     /gene="relB"
                     /locus_tag="QBF46_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HDQ6676029.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            8240..8527
                     /gene="relE"
                     /locus_tag="QBF46_003906"
     CDS             8240..8527
                     /gene="relE"
                     /locus_tag="QBF46_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HDQ6676030.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            8599..8754
                     /gene="hokD"
                     /locus_tag="QBF46_003907"
     CDS             8599..8754
                     /gene="hokD"
                     /locus_tag="QBF46_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDQ6676031.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            9213..9491
                     /locus_tag="QBF46_003908"
     CDS             9213..9491
                     /locus_tag="QBF46_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676032.1"
                     /translation="MAHDTKLHNSDDSAVFASRCGRRFHAFKSDWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRLWR"
     gene            9493..10541
                     /locus_tag="QBF46_003909"
                     /pseudo
     CDS             9493..10541
                     /locus_tag="QBF46_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            10555..11307
                     /locus_tag="QBF46_003910"
     CDS             10555..11307
                     /locus_tag="QBF46_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDQ6676033.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(11585..11674)
                     /locus_tag="QBF46_003911"
     CDS             complement(11585..11674)
                     /locus_tag="QBF46_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676034.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(11729..11941)
                     /gene="cspF"
                     /locus_tag="QBF46_003912"
     CDS             complement(11729..11941)
                     /gene="cspF"
                     /locus_tag="QBF46_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HDQ6676035.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            12242..12457
                     /gene="cspB"
                     /locus_tag="QBF46_003913"
     CDS             12242..12457
                     /gene="cspB"
                     /locus_tag="QBF46_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HDQ6676036.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT         3417 a         2795 c         3016 g         3511 t
ORIGIN      
        1 tgtcagggaa atgaatttct atattcctag cttcaacttt gcctctgtta aatactttta
       61 acctgtaatt atttctggct atttttacca agcgagcacc aagctcagcc ttcatatcat
      121 tgatggctga ctttgtttct ttttccagaa gcaacttacg taacctatct tgctcatctt
      181 tgataacctt ttgttctctt tcatttttcc ttgatctaat ctcggatgat atgatggtgt
      241 agattgatac tgcaagcgca cctaaagcga tgacgtcact tgtcgtcatt attttatcct
      301 tatattttta cttcccttaa atgctttctt cagtatgtcg ttaaactgct tctgtatatc
      361 cttcgacaac tcttctttta cttcattaac atgcgcatca atacttacgc cattctcttg
      421 gattaactca tcgttagtga attcttttcc gcatcctacg catttgacgt cctccaactc
      481 ttcggtatgc tccatttctg accctccgca tacagggcat aatagagtaa tttttctatt
      541 gtatttctca ggattcaatt tcatatattt ccacctttct tatttgccat ttttaggaat
      601 gctatggact taaaaattgg ggggtactta atttatcctt acttatcata acactgaagc
      661 tattaccttg taccgaagat ttcctcagaa cgtgactgta atgtcttcaa cgcatcgagc
      721 atatcgtcgc cgttaagtga acgttgggat ttattctcag ttggttttct tttccgccgc
      781 caggggccat ctccggcagg gagagcctga gatgtcaaag ctcagtgaaa atgtctaatc
      841 actggctcat tagaaatgtc catatgttga aaaggctgct gcgaaaatcc ctcccgcagc
      901 ggcctattta gcataacgtc attatgcgaa cctactatct tcctgcgtta cttgccgcaa
      961 gtaggacagg tatttgcatc accaaaacgc atagtacatt tgtttatact gggggcgggc
     1021 cgtctgcggg aaggctcatc accagaacaa ggtaataatt gtgagcgatt actaaacagt
     1081 aatcagttct gcgatttttg tggcaacaac tgccagcgta tcctctcctg tatccagacc
     1141 acttcgggat gcgagtaaag gactgacgtt acgaagtgct tcatacgttg tgttatgcac
     1201 gataggaacg agctggttac cagccaataa tgctgaaagt tctttatcag caacgccttc
     1261 tttaggcagg cgactcagca atgcaggggt taccaatacc aagccgattc gtgaattggc
     1321 taaaccttta tcgatggcac gcatcatagg aacaccgagg gcgagatcct tttcactgaa
     1381 ccatactttg acacctgctg cttcgagtaa atcatgtagt tctttggcta tgccttgtct
     1441 gtcatcccag gcatggcaaa gaaacacgtc acgcaaatct ggctgttcag cggcaagttt
     1501 ctcaaccact tgtcgaatcg gcgtgagtga ttgtacttgg gcagtcgtat aagatactga
     1561 agaacttgat tttgaccagc gaggcttcgt actactgctg gaaccgccgc cactcctgcc
     1621 atagcttccg ccgccgctat tgcccgaaga ggggtaagaa gaataactat cataaccgta
     1681 gctaccacga ctatagctac cacgattgcg acatgctgga caggcagccg ctgctgctga
     1741 tgagctgtgt ccacgtacag gagctgtaca tcttgccata ctcgggttcc ccttattatt
     1801 ctggaatata tctgctgtga tagtaagagc agaacagtaa tgagggttct tggttataaa
     1861 accaactggc gtgttttcag aagtttgcac tccatcacac tgctaaggga cgttatggta
     1921 aataccattc tgcaataact atccatgtat agatcatcct ttatctccgt atggcataac
     1981 ccatctgaat ccattaagca tgacaaaacc ttctttataa atagcacttt cagtagtact
     2041 tgatgtagaa aattgattag catttgtgtt tttacttgat atatccgcaa tatactgata
     2101 ttaattaaag cgtaaattat ctaaacctag attttcaata tgttataggt ggttcaaatg
     2161 ccaggtacta agtgtgttac tgttcttact gctcgtggaa ttactgagat tttaaaaaca
     2221 ggtggaagca aagcctggag actcaatgca acccatgcag gaaaatgttc gtatgttgtt
     2281 tgcgttcaga actcgaagat tgattgggga acccaggagg caaagcatca ccatgctttt
     2341 atgattggcc gtatttctgg gattagcagg attccagacc atcccaagaa gcgttggata
     2401 atcaacattg attcttatgc tgagattgat atccctgacc aatgggatgg aaaccggaat
     2461 ccggtttctt accgaaacct tgaggatatg gggatagatc ttgatactct cgattttaaa
     2521 cctgttggtc atattgtatc tggaataaaa cagactagtg gtgacgaata cgatgagtat
     2581 gaagaagttg atgtcagcga tagaccttta agcctgaaag aagctaaggc tggtctagca
     2641 cttcattttg gcgtcaatga ggatgacatt cagatcacta ttaaggggtg acctttatgg
     2701 aatcattctc tgttgagcat tggttaccac aggctactgc tggagatagt caggctagat
     2761 tttatttggg gaagatattc tcatgcgggc tgggtgttca tcaggattat aagctggcta
     2821 tatactggtt tcagcttgca gcggagcaag gccatgatga tgcccagttt aatcttgggt
     2881 gggtatatca tttggggtta ggtggatata aacatgtcca aaaagcgtct gaatggtatc
     2941 agaaggccgc tctgcaaggt catgcagagg ctcagtttca tctaggaagg ctgtatgctt
     3001 tctctgattt aagaaatgat gggcttgcag ttgaatggtt tctctgtgcc gcgaagcaag
     3061 gctatagtag tgcgcaattt aaacttggct ggatgtatca tctcggtatg ggcgttaagc
     3121 aaagtgacag gcaggctttc cattggttta ttcgggctgc tgagcaagat catgcagggg
     3181 ctcaggcatc tcttggtgag atgtataagc atgggtgggg agtcgagcaa gacgaaacga
     3241 tggcacagta ttggtttcga ctcgcaagaa caatgccata gcatcaagcc cgccctgcgg
     3301 gcttttttat gtctgcgcat aatgcggcgt tatggtaaat agcctatgct aatgtccgct
     3361 aagagcgaaa tatagtcatt cgtaccctga gaattacact ttgaagtgat tcagggtatg
     3421 cgcttactgg ttacgaatat tgagcgagaa tcatgtgatg atcgccgctt ttctttcgaa
     3481 ccagtaggtg ctccacgttc gctaacgaat actcagggca tgcagataaa ctgctggcta
     3541 tatttctttc gaagagcgtg gaatgcatac caatccccga tgaaaggagt tggtgatgac
     3601 tgcgactaat caatttgctg tacatgttgg tctggactgg gcagataaaa aacacgatgt
     3661 ctgtgttcag tttaaaaacg gtgaacgcgt attccatgtg attgaacata cagcagaagc
     3721 gcttgatgcc tggcttactg agttacacca gaaggtaaaa ggcaggatcg ctatagctct
     3781 tgagctgaag aagggacccg tggtgtatgc tcttcagaaa tatccgttta tcaccgtttt
     3841 ccccgtccac gctttgtccc tcgctcgtta ccggcaagcc ttctcaccca gcggcgctaa
     3901 agatgaccca caggatgctg agctggcatt agagttaatg ctacgttacc cccaaaagat
     3961 aaaagccatt gaacccgaca atgcggatat tcgcttactt cagcaactgg ttgaacagcg
     4021 tcgtcagctg gttgaagata aacgccgttt tgtgaaccgg ctaatcaaca cgcttaaaca
     4081 gtactatcct cagcccctgg agtggttctc acatcggggt agcttgctac tgtgcgaact
     4141 gattatgcgg tggcccagtt tgcaacaact gaaacgcgcc aggcacgata cgatacgcaa
     4201 ctttctgaat gccaaaggtg gtcgcgcaat ggcccttacc gagcaacgtg tgacgaatat
     4261 agataacgcg atcccattga ccacagatcc gagcgttata gaggctaatg ctttgatggc
     4321 tacagcactg gctacacaaa ttaaagtcgt gagtgaaatc atcaaaacct atgacgaacg
     4381 aatcgaaacg ctgtttgaca cattgccaga tgcggggctg ttcaaatcac ttccgggcat
     4441 ggggccgtgt atgggcccac gaatgcttgc tgcacttggt gataatcgtg accggtttaa
     4501 cagcgccgaa gaaattcaaa actatgcagg tattgcacca gtgacagaac gaagcggcca
     4561 gaaatcctgg gttcactggc gatggcaatg tgccaagttc gtcaggcaga cctttgttga
     4621 atgggcagcc aaaacggtta actcatcata ctgggccaga ctttattacc agggccaacg
     4681 agacaaaggg aaatcgcatc aatcagccat ccgggctctg gcgtttaaat ggataaggat
     4741 catttaccgc tgctggaaga ccagaacccg ttatgacgaa gcgaaatatt tgctggcact
     4801 cgaagcgcga aactcgccct tacttaggcc ataaaaagct tgtcgaatgt ctcagggcgt
     4861 gaagcggaag ttaaatggtt agacgtgagc atcgacttac agaggtctag cccaatgcgg
     4921 cagctttata tttgtaagat gctcttcctc aacgacaaac gccaggctgt aacgagctcg
     4981 ggtaatggcc acgtacagct tgtttctaga ttcctccgtt ttatctttat cgaacacgcc
     5041 aagatcgcca cccagatact tgagatgttt ctccggtgga ataattagta cgcggtcaaa
     5101 accgagacct ttacatgagc cgaaggtgtg acaggtgaga cctggtggca aatagccttt
     5161 tcccatgggc gatgaccatc gcaaaacttg aggtttgaat atatcgagat aatcgtccac
     5221 ctgagatggt tttatgagaa aacagccgca gtgatgggcc atctcagcag gaacttctcc
     5281 cacctctgat tgcgtttgtt cataaatacc gggatgaatg gtatccgcga gatcgcagat
     5341 ttcctggata catctgcgat tcttcgccag cgagaacgga ctaaaattga ggtctttgaa
     5401 gtattgaatc ttttgctcag gtgtttccgg tgccttgtgg ccgaacgtgg tcgtgtaaat
     5461 agtttgacga aaatcgccta cacagcaaat agagttacgc attgccttac taagtgcttt
     5521 gataacgtca aaatcccatc caaccaaatc ctgaacctca tcaaaaaaga tcctctgata
     5581 aatctctttc aatctgttag cgggggcatt ctttgttgaa gcggagattc ttgttgccag
     5641 cttcgccagc agtccagtgt aagctttggt tttgcacggc gtcagataat gaagaggatt
     5701 caacgcccca ttaatttttt cccctctttt gggatgccat ttcctcgtac caggtatcag
     5761 gtgagggtta tgctcggtga acattgtaga agtgattcga tctggaaata tggccgtttg
     5821 ataaggtctc actaagtctt ctaggtaaaa tgaaaacagg cctttcacaa caaaatcatc
     5881 gctgtgtttg ccgtacaatt ccacaaagcg agaacggagc tctgcctggt tgttcatggt
     5941 gtaggtgacg accaacacgc gtccgcccgc ctttatgact tcaatggcat cctcaatgac
     6001 tttttgggtt tttcctgatc ctgcaccggc aatccagaat tcattaggca aagtcgaaca
     6061 cctcatcgag gaaaggtgga tacctgaaat tcagagtgcc atcgaacagc ttgatagctg
     6121 aatcgacctt gcgagcgccc ttaccgtttc cctggacgct tcgaaaccag tcgatcagga
     6181 agtcgcgtct cttatctaag tcgactactt tatcgtagag gttaaacgtc tgtgtcgata
     6241 acaccacttt ggcgaaagcg tccagcgtaa ccaagtcggt tccattggca tatatcatcg
     6301 cgggttcaag cgaaaactcc tcatttttgg cagaggagat aagtttgata ttgggatagg
     6361 ccgcataatc ggcacgcact ttcacgacgt tttcatcata gtcgccatcg ttatctctga
     6421 ggacgtttat ccgggtacca atttccatac caacggagat gaacgtatca aacccgactc
     6481 cgcgcacgac gatgatatca ataccatcct gctcgggcag gcggttgtgc ttgcggaggt
     6541 aaatcttctt gagtactaac tcgtctgacg gtccctcgac aagtatgact ttgtgcgaca
     6601 gtgccacgcg caatgtgtca tagcccggta agcgctttaa ggttttgacc actgcagggg
     6661 caagcttatg aagacgtttg tatcccgact gaaccaggca gattttatca atgctcagct
     6721 tgttgagcac atacgagctg tgagtggtaa ggaagagttg tttgtcgccc cgctgatttt
     6781 caatgtagtg aaccagctta ttgaggttag tgtgagaaag gtgattttcg ggctcctcca
     6841 tcatcaccaa atcgatatcc ttcgacttgt tctgaatcgc gagcttgatc tgcacattac
     6901 tctgctcacc cttgccaatg aactggaaag ggacatcatc tacttcaagt tgaaggctgg
     6961 tctgaataga tcccgcaggt aaagtgcttg cggcaatgga cagctttttt tctgtgatca
     7021 agtggtccgt atccagaccg gtgttgactg tcctgacctc acctgagttg ttaaaaacct
     7081 gctgattctc acgataattg agggtcagct taaccagttc ctctttcttt aaagcagtat
     7141 taagtatggt agagatgtac tgattcttac ccagagtggg gtggatacga gttgggtcga
     7201 tgtataacgc cctgaatttc ttcgctacgg ctttaacggg attccatccg aaatccagcc
     7261 attccagctt gtagaattct atcgggatac tcgtaatgtg tgggtttgtc agtaagtggt
     7321 cggcgtaaac gtccttgagc gtgtcatcaa agcgcaccag caccgtgatc ccttgagcat
     7381 cggctttaag gaaattattt gtaccccggt attcaggcac cccctcaatg aatgcctcta
     7441 ttgccaagac aggcaagtgc gtggccgatt tatctgaagc caaaaaaagc tgcactgcat
     7501 ccttattaaa aatgtctggc gtaaactcgc tgttgaaggg gcggccccga tagctgtaat
     7561 taagtacgat ttccagggct tccagaatag tacttttacc ggcgttgtta tcacctacaa
     7621 agatatttac gtcatcgttg aattcaaaat gctcatcttt gaatttcttg aaattggtaa
     7681 gcttaatcga tttaacccgc actctaagtc cctcaagata ctgtcaaatg atacccctta
     7741 aagacattaa ctcaccgata tacctgtgtc catatgcagg ctcgttgtcc ccttaaatcc
     7801 tgcaggcact gaagtggtgg tcgacataaa taggccaaga tatgagagct gttcttggac
     7861 aacttaaagt ctgctcttgg cacaaagcgg acagtgatca ccgttcttac gactactttc
     7921 tgacttactt cgtgacttgc cctaagcatg ttgtagtgcg atacttgtaa tgacatttgt
     7981 aattacaagg ggtgtaagac atgggtagca ttaacctgcg tattgacgat gaacttaaag
     8041 cgcgttctta cgccgcgctt gaaaaaatgg gcgtaactcc ttctgaagcg cttcgtctca
     8101 tgctcgagta tatcgctgac aatgaacgct tgccgttcaa acagacactc ctgagtgatg
     8161 aagatgctga acttgtggag atagtgaaag aacggcttcg taatcctaag ccagtacgtg
     8221 tgacgctgga tgaactctga tggcgtattt tctggatttt gacgagcggg cactaaagga
     8281 atggcgaaag ctgggctcga cggtacgtga acagttgaaa aagaagctgg ttgaagtact
     8341 tgagtcaccc cggattgaag caaacaagct ccgtggtatg cctgattgtt acaagattaa
     8401 gctccggtct tcaggctatc gccttgtata ccaggttata gacgagaaag ttgtcgtttt
     8461 cgtgatttct gttgggaaaa gagaacgctc ggaagtatat agcgaggcgg tcaaacgcat
     8521 tctctgaacc aaagcatgac atctctgttt cgcaccgaag gtgacacttc tgctttgcgt
     8581 tgacaggaga agcaggctat gaagcagcaa aaggcgatgt taatcgccct gatcgtcatc
     8641 tgtttaaccg tcatagtgac ggcactggta acgaggaaag acctctgcga ggtacgaatc
     8701 cgaaccggcc agacggaggt cgctgtcttc acagcttacg aacctgagga gtaagagacc
     8761 aggcgaggga gaaatccctc gccacctctg atgtgtcagg catcctcaat gcacccgcac
     8821 ttaacccgct tcggcgggtt tattttatct gtaaatattt ttataaaaat aatgcccaca
     8881 cacagcataa aacaaaaagt atcacagata aaaaaggagc gtaatgtgca gatttgttgt
     8941 tttccatatt tactcacttt aacataatca atattgatat ggttgttgtt ttggtggttt
     9001 caaacgagat gttatggtga tctggtaaaa ttgcataaca ttaaaattta atttatctaa
     9061 tcgcttttaa taataagcgt tgtgtttatc ccaacaatct gttgtttgac ttttattcca
     9121 ttaatgtagg ggctttacac tggaaccagt ttatttatac tttatacgcc agcctgaaca
     9181 actggcacct gctgcgccag cagagacaac cgatggcgca cgataccaaa ttacacaatt
     9241 ctgatgattc tgccgtcttt gccagcaggt gcggacggcg ttttcatgca ttcaaatcgg
     9301 actggttcca gcatccacca tgcactgaag agcaggctga atggataatt cagtgttacc
     9361 gcaggcgcgg atacgaggtt aaaaaagccc ttagcctcga ctaccgtcac tggataatct
     9421 cagtcagatt gccttactcc gaacgcccac cgcgtccgtc ccgtacattc cagcaacgcc
     9481 tctggaggta acgtgcgggt attacttcga cctgttctgg taccggaact cgggctggtg
     9541 gtcgttaagc cgggccgtga atccatgccg gtattccaca atacccgggt actggtggag
     9601 ccggaaccga aaagcatgcg taatctgccg tccggggtcg ttcctgccgt tcgccagccg
     9661 ctggtggaag acaaaacatt gctgccgttt ttcagtaaca cacgggtaat tcgtgctgct
     9721 ggtggtgctg gtgcattgtc tgactggctg ttgcgccata ttaaatcctg ccagtggcca
     9781 cacggcgatt atcatcacag cgaaaccgtt attcaccgat atggtaccgg cgcaatggtg
     9841 ttgtgctggc actgcgacaa ccagctgcgt gaccagacat ccgaatcact cgagcaactt
     9901 gctcatcaaa acctgtcagc atggatgatt gacgtcatcg gtcacgcaat aagcggtacg
     9961 caggagcgtg aattatctct ggctgaattt cctggtgggc ggtccgcaat cagctggcgg
    10021 acgcgctacc ggaagcggta ttacgtcgct cgctgggatt accagcggaa aaaatctgct
    10081 cggtgtaccg cgaaagcgac atcgtaccgg gagagcagac cgccaccagc atattgaaac
    10141 agcgcacaaa aaatcttgca ccgttgcctc acgcccacca gcaacaaaac ccatcacagg
    10201 aaaagacggt ggtaagcatc accgttgatc cagagtctcc ggaatctttc atgaagctgc
    10261 ctaaacgtca ccggtgggtt aaggagaaat acacacgctg ggttaagaca cagccgtatg
    10321 cttgctgcgg tatgccagcc gacgatccgc atcatctgat tggtcacggg cagggcggaa
    10381 tgggaacaaa agcacatgat ctctttgtgt tgcctttgtg cagaaagcat cacaacgagc
    10441 tgcatacgga tacagtggca tttgaagaga agtatggctc ccaactggag ctgatatttc
    10501 gttttatcga tcgcgcgctg gcaattggcg tactggcgta agtggagaac gagcatgaac
    10561 cttgaagcct taccaaaata ttactcccca aaatctccaa aattgagcga tgacgctcca
    10621 gcgacaggca ccggttgttt aacaattacg gatgtaatgg cagcgcaggg gatggtgcag
    10681 tcgaaagcac cacttgggtt ggccttattt ctggcaaaag ttggtgttca ggaccctcag
    10741 tttgcgattg aaggcctgct aaattacgcg atggcactgg ataacccgac attgaacaaa
    10801 ttgagtgaag aaatccggtt acagattatt ccttacctcg tgagttttgc ctttgctgat
    10861 tactccaggt ctgcggcaag taaggctcgc tgtgagcatt gttcaggtac gggattttat
    10921 aatgtattgc gcgaagtggt gaaacactac agacgcgggg aatctgtaat caaggaagaa
    10981 tgggtgaagg aactatgtca gcattgccat ggtaagggcg aagccagcac agcgtgcaga
    11041 gggtgtaagg gtaaagggat tgttctggat gaaaaaagaa cccggtttca tggcgtaccg
    11101 gtatataaga tttgtgggcg ttgtaatgga aaccggttta gtcgtttacc gaccacgctg
    11161 gcacgacgtc atgtccagaa gctggtacca gacctgaccg attatcagtg gtataagggg
    11221 tatgcggacg tcattggtaa actggtaaca aagtgctggc aggaagaagc atacgcggaa
    11281 gcgcaattga ggaaggtgac gagataaatg atttttgctg aagatggcga catgatgttt
    11341 gcatttttca aaaaatatgg ataaaatttt ttcaacgatg ggctttgtat acccgacgtt
    11401 aagaaaaagt agaaaacccg ctgatgagcg ggttttgtgc tttaaatggg gcaatggtaa
    11461 tgttgaatct catcccggga ctcatgtctg ttaacttatt atttagctgg tgacttggtt
    11521 atttgcctga tgtttaaaat gttttcttcc agtacaatgt ccctaaacac aatgagtctg
    11581 cttattatat tattagcaga gctattacgg ccaaagtaca gcataagctt ttaaagccaa
    11641 tcaaccagtc atcaagacag acggggttat tcataaaaac tctccatgtg tgatccgatg
    11701 gggcctgaaa ttaaagcttt aatatagctc atgaaaggta aacattggca gctgaagggc
    11761 cacgcagacc atttatccgg caaaattcca cgcgtaatcc ggtggtaatt tcttctgcat
    11821 cgcggagatt gagcgctgaa acatgaagct ggacatcgat acgaccatcg gatggggtga
    11881 taagaccctt gccgcttttg ccgtcaaagg ttttgacaat tcctgtcatt ttacgggaca
    11941 aaaaaattcc ttaatactga taacttggcg cactatacac acgttcctga agaaagctat
    12001 agttttttga tggggttgaa gatggctgga tgtctaaaat aaacattgct tcatatgttc
    12061 aactatgcgt taatgattgc gtcggtttga agaacagacg atatacgaag tagtttacta
    12121 aagcagttct catttcaggt gttattcact tattccttct ttgagtctct ccaattaagt
    12181 acgaagtcgt ttctgttatg caaaccattt atgccgaaag gctcaagtta aggaatgtag
    12241 aatgtcaaat aaaatgactg gtttagtaaa atggtttaac gctgataaag gtttcggctt
    12301 tatttctcct gttgatggta gtaaagatgt gtttgtgcat ttttctgcga ttcagaatga
    12361 taattatcga accttatttg aaggtcaaaa ggttaccttc tctatagaga gtggtgctaa
    12421 aggtcctgca gcagcaaatg tcatcattac tgattaaaat tcatcgctcg tctgtatacg
    12481 ataacgaaga aggctgatgc ctgagtagag atacggacag agtagtgaat attggatttc
    12541 tttaataaaa attaaggagg tccaatacat gaaacaatgg ccagcatatt tggcaaaatc
    12601 ttaatcagga aaagtatgct aaccattgtg gtgaagtgca ggtttgctgc atgaatagtt
    12661 ttacagcaga agctaactgc tggcatggca aaacaaagtg cgtaagtgga tgactcccac
    12721 aaaaagcacc acaatctca
//
LOCUS       DAOFQD010000134        12733 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.134, whole genome shotgun sequence.
ACCESSION   DAOFQD010000134 DAOFQD010000000
VERSION     DAOFQD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.134"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            303..1442
                     /gene="wza"
                     /locus_tag="QBF46_003914"
     CDS             303..1442
                     /gene="wza"
                     /locus_tag="QBF46_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDQ6676037.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1448..1891
                     /gene="wzb"
                     /locus_tag="QBF46_003915"
     CDS             1448..1891
                     /gene="wzb"
                     /locus_tag="QBF46_003915"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDQ6676038.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1894..4056
                     /gene="wzc"
                     /locus_tag="QBF46_003916"
     CDS             1894..4056
                     /gene="wzc"
                     /locus_tag="QBF46_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDQ6676039.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFAVC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4149..4988
                     /gene="wcaA"
                     /locus_tag="QBF46_003917"
     CDS             4149..4988
                     /gene="wcaA"
                     /locus_tag="QBF46_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDQ6676040.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTVLNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            4991..5479
                     /gene="wcaB"
                     /locus_tag="QBF46_003918"
     CDS             4991..5479
                     /gene="wcaB"
                     /locus_tag="QBF46_003918"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDQ6676041.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5476..6693
                     /gene="wcaC"
                     /locus_tag="QBF46_003919"
     CDS             5476..6693
                     /gene="wcaC"
                     /locus_tag="QBF46_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDQ6676042.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCEKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6668..7888
                     /gene="wcaD"
                     /locus_tag="QBF46_003920"
     CDS             6668..7888
                     /gene="wcaD"
                     /locus_tag="QBF46_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDQ6676043.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAMEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            8014..8760
                     /gene="wcaE"
                     /locus_tag="QBF46_003921"
     CDS             8014..8760
                     /gene="wcaE"
                     /locus_tag="QBF46_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDQ6676044.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVPGLKKW
                     RYDLEYKISSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8776..9324
                     /gene="wcaF"
                     /locus_tag="QBF46_003922"
     CDS             8776..9324
                     /gene="wcaF"
                     /locus_tag="QBF46_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDQ6676045.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9352..10473
                     /gene="gmd"
                     /locus_tag="QBF46_003923"
     CDS             9352..10473
                     /gene="gmd"
                     /locus_tag="QBF46_003923"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDQ6676046.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10476..11441
                     /gene="fcl"
                     /locus_tag="QBF46_003924"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10476..11441
                     /gene="fcl"
                     /locus_tag="QBF46_003924"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDQ6676047.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11444..11923
                     /gene="gmm"
                     /locus_tag="QBF46_003925"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11444..11923
                     /gene="gmm"
                     /locus_tag="QBF46_003925"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDQ6676048.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11920..>12733
                     /gene="wcaI"
                     /locus_tag="QBF46_003926"
     CDS             11920..>12733
                     /gene="wcaI"
                     /locus_tag="QBF46_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDQ6676049.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGTATVWRCPLYVPKQLSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNIIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGG"
BASE COUNT         3169 a         3174 c         3411 g         2979 t
ORIGIN      
        1 accttatatt cggaagttaa tttccgcaat aaccctttct catcaacgat tgtatgcaga
       61 gagggtttga aaaattaagc taaatcagtg tattggtagc taaaaagcca ggggcggtag
      121 cgtgtctgga tgcctgaaag accagtctga attattctgt caatagcctg cggatgaaat
      181 caattttttt aggactgatg ccagttaaat ttttattcaa ttaactgcaa ggtaaatgtg
      241 catggcgaca ttgctattaa tagtgcacag gataattact ctgccaaagt gataaataaa
      301 caatgatgaa atccaaaatg aaattgatgc cattattggt gtcagtaacc ttgataagcg
      361 gttgcacagt acttccgggc agcaatatgt cgacgatggg caaagacgtc atcaaacagc
      421 aggacgctga tttcgatctc gacaaaatgg tgaatgttta tccgctgacc ccgcgcctga
      481 ttgaccaatt acgcccacgc ccgaatgtag cgcgccccaa tatgacgctg gaaagtgaga
      541 tcgcgaatta ccagtatcgc gtcgggccgg gggacgttct taatgtcacc gtctgggatc
      601 acccggaact caccacgcca gccggtcagt accgcagctc cagcgacacc ggcaactggg
      661 tacagcctga cggcactatg ttttacccgt atatcggcaa ggttcacgta gtcgggaaaa
      721 cgctcgctga aatccgcagt gatattaccg ggcgcttagc gacgtacatc gctgacccgc
      781 aggtggacgt taatatcgcc gccttccgct cacaaaaggc ctatatctca ggtcaggtga
      841 ataaatccgg tcaacaggcg atcaccaacg taccactgac cattctcgac gccatcaacg
      901 ccgcaggtgg cctgaccgac accgctgact ggcgcaacgt ggtgctaaca cacaatggtc
      961 gtgaagaacg catttctttg caggcgctga tgcaaaacgg cgacctcaat cagaaccgac
     1021 tgctttatcc cggcgatatt ctctacgtgc cgcgtaatga tgatctgaaa gtatttgtga
     1081 tgggtgaagt gaagaaacag agcaccctga aaatggactt tagcggcatg accctgactg
     1141 aagctctggg caatgccgaa ggcatcgaca tgaccacctc caacgccagc ggcatctttg
     1201 tcattcgtcc gttgaaaggc gagggcgggc gtaacggcaa gattgccaat atctaccagc
     1261 tggatatgtc cgatgcgacg tcgctggtga tggcgacaga attccgcctg caaccttatg
     1321 acgtggtgta tgtcaccacc gccccggttt cccgctggaa ccgtctgatc aatcagttgc
     1381 tgccaactat tagcggtgtc cgttacatga cggatacagc cagcgacatt cataactggt
     1441 aatcgtcatg tttaacaaca tcttagttgt ctgtgtcggc aatatttgcc gttccccgac
     1501 ggcggaacgc ttactgcaac gttatcaccc ggagctgaaa gtggagtccg ctggactcgg
     1561 cgcgctggtc ggtaagggcg ctgatcctac cgctatcagc gtcgccgcag aacatcaact
     1621 gtctctggaa ggtcactgtg cacggcaaat cagccgccgt ctgtgtcgca actatgacct
     1681 gattttgacc atggaaaagc gccatatcga acgcttatgc gagatggcac ctgagatgcg
     1741 cggcaaagtg atgctgtttg gtcactggga taacgaatgt gaaatccccg atccgtatcg
     1801 caaaagccgg gaaacgtttg cagcggtgta cacattactt gaacggtctg cccgccagtg
     1861 ggcgcaggca ttgaacgcag agcaggtata agaatgacag aaaaagtaaa acaacatgcc
     1921 gctccggtaa cgggcagtga tgaaatcgat attggtcgcc tggtcggcac cgtcattgaa
     1981 gcgcgctggt gggtgattgg catcaccgcc gtatttgccg tctgtgccgt ggtttacacc
     2041 ttcttcgcca cgccgattta tagtgccgac gcactggtac aaatcgagca aaacagcggc
     2101 aattcgttag tgcaggatat cggatcggcg ttagccaaca aaccgcctgc atcggacgcc
     2161 gagatccagt tgattcgttc gcgcctggtg cttggtaaaa cggtggacga tctcgacctc
     2221 gatattgcgg tgagcaaaaa cacgttcccg attttcggtg cgggctggga tcgcctgatg
     2281 ggacgtcaga acgaaacggt gaaagtgacc acctttaacc gcccgaaaga gatggcggat
     2341 caggtgttta cgcttaatgt gctggacgat aaaaactaca ccctgagcag cgatggcggc
     2401 tttagcgccc gtgggcaagc gggccagata ctgaaaaaag aaggcgtcac gctgatggtt
     2461 gaagccattc acgccagccc gggcagtgag tttaccgtca ccaaatactc cacgctgggg
     2521 atgatcaacc aactgcaaaa cagcctgacg gtaacggaga acggcaaaga cgcaggcgta
     2581 ctgagcctga cttataccgg tgaagatcgc gaacagatcc gcgacattct taacagcatc
     2641 gcccgtaact atcaggaaca aaatattgag cgcaaatcgg cggaagcgtc gaaaagcctc
     2701 gctttcctcg cgcaacagtt accggaagta cgtagccgcc tcgatgttgc cgaaaacaaa
     2761 ctgaatgcct tccgtcagga taaagattct gttgatctgc cgctggaagc gaaagcggtg
     2821 ctcgattcga tggtgaacat cgacgcgcag ttgaacgaac tgacctttaa agaggcggaa
     2881 atctccaagc tgtacaccaa agttcacccc gcgtaccgca cgctgctgga gaaacgtcag
     2941 gcgctggaag acgaaaaagc caaacttaat ggtcgcgtaa cggcgatgcc gaaaacccag
     3001 caggaaattg tccgtctgac ccgcgatgtc gagtctggtc agcaggtcta tatgcaactg
     3061 ctgaataaag agcaggagct gaaaatcacc gaagccagca ccgtcggcga tgtgcgcatt
     3121 gttgacccgg caatcaccca gccaggtgta ctgaaaccga agaaagggct gattatcctt
     3181 ggggcgatta ttcttggcct gatgctctct atcgtgggtg tgctgctgcg ctcgttgttt
     3241 aatcgcggca tcgaaagccc gcaggtgctg gaagaacacg gtatcagcgt ctatgccagc
     3301 atcccgttgt cggaatggca gaaagcgcgc gatagcgtca aaaccatcaa agggattaaa
     3361 cgctataaac agagccagct actggcggtg gggaatccaa ccgatctggc gattgaagcc
     3421 atccgcagtc tgcgtaccag tttgcacttc gcgatgatgc aggcacagaa caatgtgttg
     3481 atgatgaccg gggttagccc gtcaatcggt aaaacctttg tctgcgccaa cctggcggcg
     3541 gtgatcagcc agaccaataa acgggtgttg ttgatcgact gcgatatgcg caaaggctac
     3601 acccacgagc tgctgggcac taataacgtt aatggcctgt cggaaattct gattggtcag
     3661 ggcgatatta ctaccgctgc taaaccgacc tctattgcca aatttgacct gatcccgcgc
     3721 ggtcaggtgc cgccaaatcc ttctgaactg ttgatgagcg aacgctttgc cgaactggtg
     3781 aactgggcga gtaaaaacta cgacctggtg ttgattgata cgccgccgat tctggcagtg
     3841 accgatgcgg caattgttgg tcgtcatgtc ggaaccacgt taatggtggc gcgttatgcg
     3901 gtcaacacat tgaaagaagt ggaaaccagt ctgagccgct ttgagcaaaa tggtattccg
     3961 gtgaaagggg tgattctgaa ctccatcttc cgccgcgcca gcgcgtatca ggattatggc
     4021 tattacgaat acgaatataa gtcggatgcg aaataacgac gcctgcattc gcaccgcccc
     4081 gtaggccgga taaggcgctc acgccgcatc cggcaagcaa accagctcat aagccgggag
     4141 aacaacctat gaaagacaat ccgctgatct caatctatat gccaacctgg aaccgccaac
     4201 aactggcgat tcgggcgata aaatcggtcc tgcgccagga ctacagcaac tgggagatga
     4261 tcatcgtgga tgattgctcc acctcctggg aacaactgca acagtacgtc actgtcctca
     4321 acgatccgcg tatcacttac atccacaacg acattaacag cggggcgtgc gcggtgcgta
     4381 accaggcgat tatgctggcg caaggggaat acatcaccgg gatcgatgat gatgatgaat
     4441 ggacgcccaa ccgcctgagc gtcttcctcg cccataaaca gcaactggtc actcacgcct
     4501 ttttgtacgc taacgactac gtttgccagg gcgaagtcta ttcccaacca gcaagtttgc
     4561 cgctgtatcc caaatcgccg tattcacgcc gcctgttcta taaacgcaat atcattggca
     4621 atcaggtctt tacctgggcg tggcgtttca aagaatgttt gttcgatacc gaactgaaag
     4681 cggcacagga ttacgacatc ttcctgcgga tggtggtgga gtatggcgaa ccgtggaaag
     4741 tggaagaagc gacgcagatc ctgcatatca atcacggtga aatgcagatc acctcgtcac
     4801 cgaaaaaatt ctccggatac ttccactttt accgcaagca caaagacaaa ttcgaccgtg
     4861 ccagtaaaaa atatcagctg tttaccctct accagatccg caataaacgt atgacctggc
     4921 gcactttgct gacgctgctg tcggtgcgta acggcaaacg tctggctgat ggtattcggg
     4981 ggcgctaaag atgctggaag atctacgcgc caacagctgg agtttacgcc cgtgctgcat
     5041 ggttcttgcc tatcgcgtcg ctcatttttg ttcggtgtgg cgcaaaaaga acgttctcaa
     5101 caatctgtgg gcggccccgc tgctggtgct gtatcgcatt atcaccgaat gctttttcgg
     5161 ttatgaaatc caggctgccg cgaccattgg ccgccgcttt actatccatc acggttacgc
     5221 cgtagtgatc aataaaaacg tggtagcggg ggatgatttc accattcgcc acggcgtcac
     5281 tatcggcaat cggggggccg ataacatggc atgtccgcac attggcaacg gcgtcgaact
     5341 cggtgccaac gtcattattc ttggtgatat cacgcttggt aacaacgtca ccgtgggcgc
     5401 gggcagcgtg gtgctcgact ctgtcccgga caacgcgctg gtggtgggag aaaaagcgcg
     5461 agtgaaggta attaaatgaa tattctgcaa tttaatgtgc gactggcgga aggtggggcg
     5521 gcaggtgtgg cgttagatct ccaccagcgt gcgctgcaac aggggctggc gtcacatttt
     5581 gtctacggct acggcaaagg cggcaaagag agcgtcagcc atcagaacta tccgcaggta
     5641 atcaaacata cgccgcggat gaccgcaatg gcgaatattg ccctgtttcg tctgtttaat
     5701 cgcgatctgt tcggcaattt caatgagtta tatcgcacca ttacccgcac cccggggccg
     5761 gtggtcctgc atttccatgt gctgcatagc tactggctaa atcttaagag cgtggtgcgc
     5821 ttttgcgaaa aagtgaaaaa ccataaaccg gacgtcactc tggtctggac gctgcacgac
     5881 cactggagtg taaccgggcg ctgcgccttt actgacggtt gtgaaggctg gaaaacgggc
     5941 tgcgagaaat gcccgacctt aaataactat ccgccggtga agattgatcg cgcacaccag
     6001 ctggtggcgg gcaaacgcca gttattccgt gagatgctgg cgctgggttg tcagtttatt
     6061 tcccccagcc agcatgtggc tgatgctttc aatagtctgt acggtccagg gcgttgccgg
     6121 atcatcaata atggtattga tatggcaacc gaagcgattc tggcggactt gcctccggtg
     6181 cgcgaaaccc agggcaagcc gaaaatcgcg gtggtggcgc atgacctgcg ttacgacggc
     6241 aagactaacc agcaactggt gcgtgagatg atggcgctgg gcgacaaaat cgaactgcat
     6301 acctttggta agttctcgcc gttcaccgct ggcaacgtgg ttaatcacgg ctttgaaact
     6361 gacaagcgca agttgatgag cgcgctcaat cagatggatg cgctggtatt cagttctcgc
     6421 gtcgataact acccactgat tttgtgtgag gcgttatcaa ttggcgtgcc ggtgattgcc
     6481 acccatagcg acgcggcacg tgaagtgttg caaaaatccg gcggtaaaac cgtcagcgaa
     6541 gaagacgtgc tgcaactggt gcagttaagc aaaccggaaa tcgcgcaggc gatatttggt
     6601 accacgctgg ctgagttcag ccaacgcagc cgcgcggcct acagtggaca acagatgctg
     6661 gaggagtatg tcaacttcta tcagaatctg tagctacctg ctgctgccgc tgatttacct
     6721 gctggttaac gtcaaaatcg cccagcttgg cgaaagtttc cccatcacca tcgtcacttt
     6781 tttacctgtt ttgttactgc tgtttttaga acgcatcagc gttaaaaaat tgatgattgc
     6841 cttagggatt ggcgcgggac tcacggcgtt taactacctg tttggtcagt cgttggatgc
     6901 cagtaaatac gtcacttcaa ccatgctgtt tgtctatatt gtgatcatta ttggcatggt
     6961 gtggagtatt cgctttaaaa cgatttcgcc acacaaccat cgcaagattt tacgtttctt
     7021 ttatctggtg gtcgggctgg tggtggcgct ggcggcgatg gagatggcgc aaattatcct
     7081 caccggtggc agcagtatta tggagtcgat ttcgaaatat ctgatttaca gcaacagcta
     7141 tgtgctgaat ttcatcaaat tcggcggcaa gcgcacgaca gcactttatt tcgaaccggc
     7201 atttttcgct ctggcattaa tctcaatttg gctcagcatc aaacagtttg gtatcaaaac
     7261 gcctaaaacc gatgctatga ttctcgcagg gataatatta tccggatcgt tttcaggggt
     7321 tatgaccttt atcctgtttt atttgctgga gtgggcattt caatatctga ataaagaggc
     7381 gattaagaaa aagttaccgt tagcattgat ttctctggct gtattcctgg ttggtgtggt
     7441 aatcgcgttt ccttatattt ccacccgtct gggcgattta ggtacggaag gatcgtcatc
     7501 gtattatcgt attgtcggtc cgctggtgat ggtcggttat tctttgaccc atattgacgg
     7561 tgtagtcaga tttggctcac tttatgaata tgtcgcatca ttcggaatat ttaacggtgc
     7621 ggatgtcgga aaaaccatag acaatggttt gtatctgctg attatttatt tttcctggtt
     7681 cgcggtgttt ttatcgctgt ggtacatggg gaaagtgata aaaatgatga tcaacgcttt
     7741 tggtgataac cgcaattttc gcgtgcaatt atatcttttt actccggttt cgctgttttt
     7801 taccggttcg atatttagcc cggaatatgc atttttaatc gtctgtccgt ttattttgcg
     7861 aaaagcgtta aatatacacc ctaaataatt cgagttgcag gaagttggtg acgcaacgaa
     7921 tccccagaag cgtacacgag tacgtgactg gggtgagcga ggaaaaccaa cgcacatgcc
     7981 gcttgaagta tgacgggtat tacgaggtaa aacatgttgc ttagcataat cactgtcgcg
     8041 tttcgtaacc tcgaagggat agtcaaaaca cacgcctcgc tggcgcatct ggcgcaggcg
     8101 gacgatatca gcttcgaatg gattgttgtc gatggcggtt ccaatgacgg cacccgtgag
     8161 tacctggaaa atctcaatgg tatctataac ctacgctttg tcagcgagcc agataacggt
     8221 atctacgacg ccatgaacaa aggcattgcg atggcacaag gcaagttcgc gttgtttctc
     8281 aactcgggcg atatttttca tcaggatgcc gcatattttg tccgtaagtt aaaaatgcaa
     8341 aaagataacg tgatgatcac cggcgatgcg ctgctggatt ttggcgacgg gcataaaatt
     8401 aaacgtagcg ccaaaccagg ttggtatatt tatcacagcc tgcccgccag tcatcaggcg
     8461 atatttttcc cggtacccgg tttgaaaaaa tggcgttatg acctggaata taaaatttct
     8521 tctgattacg cgctggcagc caaaatgtat aaagcaggtt atgcatttaa aaaactcaat
     8581 ggcctggtgt ctgaattttc tatgggtggg gtatctacca ccaataatat ggaattgtgt
     8641 gctgacgcca aaaaagtcca acggcaaata ttacatgtgc ctggcttctg ggcagaatta
     8701 tcctggcatt tacgccaacg tactacctca aagacgaaag ccttatataa caaaagctga
     8761 atataaggga aaacgatgca agatttaagc ggtttctcgg tgccgaaagg gttccggggc
     8821 ggcaacgcta ttaaagtgca attgtggtgg gcagtacagg caacattatt tgcctggtcg
     8881 ccacaagtat tgtatcgctg gcgggcattt ttattacgtt tattcggcgc aaaaatagga
     8941 aaaaacgtag ttattcgtcc gtcagtaaaa attacctatc cgtggaaatt aaccttaggt
     9001 gattacgcgt gggtcggcga tgacgtcaat ttatataccc tcggtgaaat aaccattggc
     9061 gcacattcgg tgatatcgca aaaaagttat ttatgcaccg ggagccacga ccatgcaagt
     9121 caacatttca ccattaacgc cacgcctatt gtgattggcg agaaatgctg gctggcaacc
     9181 gatgtttttg ttgccccagg cgtcacgatt ggcgacggca ccgtcgtggg tgcgcgaagc
     9241 agtgttttta aatcgcttcc ggcaaatgtg gtttgccggg ggaatcccgc agtggtgata
     9301 cgcaaacgcg ttgaaactga ataaattcaa aaaaatacag aggaataaga catgtcaaaa
     9361 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
     9421 gaaaaaggtt acgaggtgca tggtattaag cgtcgtgcat cgtcattcaa caccgagcgc
     9481 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
     9541 gacctgagtg atacctccaa cctgacgcgc attttgcgtg aagtgcagcc ggatgaagtg
     9601 tacaacctgg gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgca
     9661 gacgttgatg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     9721 aagaaaaccc gtttctatca ggcttccacc tctgaactgt acggtctggt gcaggaaatt
     9781 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     9841 gcctactgga tcaccgttaa ctaccgcgaa tcctatggca tgtacgcttg taacggtatt
     9901 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgaaa aatcacccgc
     9961 gcaatcgcca acatcgccca ggggctggag tcgtgtctgt acctcggcaa tatggattcc
    10021 ctgcgtgact ggggccatgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
    10081 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagttcgtg
    10141 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
    10201 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgtgaaacc gggtgatgtg
    10261 attatcgccg ttgatccgcg ttacttccgt ccggctgaag ttgaaacgct gctcggtgac
    10321 ccgaccaaag cgcacgaaaa actgggctgg aaaccagaaa tcaccctcag agagatggta
    10381 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
    10441 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
    10501 ctggtcatcg cgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
    10561 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gcggtgcatg
    10621 atttctttgc cagcgaacgt attgaccagg tctatctggc ggcggcgaaa gtgggcggca
    10681 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
    10741 acatcattca cgccgcgcat cagaacgacg tgaacaaact gctgtttctc ggatcgtcct
    10801 gtatctaccc gaaactggca aaacagccga tggcagaaag cgaattattg cagggcacgc
    10861 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
    10921 cttacaaccg ccagtatgga cgcgattacc gctcagtcat gccgaccaac ctgtatggcc
    10981 cgcatgacaa cttccacccg agtaattcgc atgtgatccc tgcattgctg cgtcgcttcc
    11041 acgaggcgac ggcacagaat gcaccggacg tggtggtatg gggcagcggt acaccgatgc
    11101 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
    11161 atgaagtctg gctggagaac acccagccga tgctgtcgca cattaacgtc ggcacgggcg
    11221 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggcc
    11281 gggtggtttt tgatgccagc aaaccggatg gcacgccgcg caaactgctg gatgtgacgc
    11341 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
    11401 accagtggtt ccttgagaat caagaccgct ttcgggggta atgatgtttt tacgtcagga
    11461 agactttgcc acggtagtgc gctccactcc gcttgtctct ctcgacttta ttgtcgagaa
    11521 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
    11581 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
    11641 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
    11701 gcacttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
    11761 ttttcgcttc agagtcgctg aagaagatct gttactgccg gatgaacagc atgacgatta
    11821 ccgctggctg acgccggacg cactgctcgc cagtgacaat gttcacgcta acagccgcgc
    11881 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
    11941 attaactact cgccggagtt aaccggtatc ggcaaataca ccggagagat ggtggaatgg
    12001 ctggcggcac aaggtcatga ggtgcgggtt attaccgcac cgccttacta cccgcaatgg
    12061 caggtgggcg agaactattc cgcctggcgg tacaaacgag aagaggggac cgccacggtg
    12121 tggcgctgcc cgctgtacgt accaaaacag ctgagcaccc tgaaacgcct gttgcatctc
    12181 ggcagttttg ccgtcagcag ttttttcccg ctgatggcgc aacgtcgctg gaagccggag
    12241 cgcattatcg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgtct gctggcgaaa
    12301 ctctcgggcg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
    12361 ctgggccttg ccggaaaagg caaaggcggc aaagtggcgc agctggcgac ggcgttcgaa
    12421 cgtagcggac tgcataacgt cgataacgtc tccacgattt cgcgttcgat gatgaataaa
    12481 gccatcgaaa aaggcgtggc ggcggataac atcattttct tccccaactg gtcggaaatc
    12541 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
    12601 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
    12661 aacgttattg aagctgccga tcgcctgcgc gatgaaccgc tgatttttgc cattgtcggg
    12721 cagggcggcg gca
//
LOCUS       DAOFQD010000135        12650 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.135, whole genome shotgun sequence.
ACCESSION   DAOFQD010000135 DAOFQD010000000
VERSION     DAOFQD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.135"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            130..1227
                     /gene="ompC"
                     /locus_tag="QBF46_003927"
     CDS             130..1227
                     /gene="ompC"
                     /locus_tag="QBF46_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDQ6676050.1"
                     /translation="MKKITVALSTVAASVLMAMSAQAAEIYNKDGNKLDLYGKVNAKH
                     YFSSSASDDGDKTYARMGFKGETQINDQLTGFGRWEYEFKGNRSETEGSGKDKTRLAF
                     AGLRFGDYGSLDYGRNYGVAYDVGAWTDVLPEFGGDTWTQTDVFMTKRATGVATYRNN
                     NFFGLVDGLNFALQYQGKNDSAARDEVESNGDGFGLSATYDYEGFGVAATYAKSDRTD
                     GQVSYAKDSPLPLNASGKTAEVWATGLKYDANNIYLATTYSETQNMTVFGDDHIANKA
                     KNFEAVAQYQFDFGLRPSIAYLHSRGENIGAFGNQDLVEYIDVGATYYFNKNMSTFVD
                     YKINLNDESDFTKKSGVATDNIVAVGMTYQF"
     gene            complement(1461..1953)
                     /locus_tag="QBF46_003928"
                     /pseudo
     CDS             complement(1461..1953)
                     /locus_tag="QBF46_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2123..2680
                     /locus_tag="QBF46_003929"
     CDS             2123..2680
                     /locus_tag="QBF46_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDQ6676051.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGSAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAVFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLVNE"
     gene            complement(2972..3991)
                     /locus_tag="QBF46_003930"
     CDS             complement(2972..3991)
                     /locus_tag="QBF46_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HDQ6676052.1"
                     /translation="MTDVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKTMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYGKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEKTAGIRDDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESLIKVGARNLPVTKKIKEVPQAANVQAPRKTSIITFNNS
                     INPFK"
     gene            complement(3984..5693)
                     /locus_tag="QBF46_003931"
     CDS             complement(3984..5693)
                     /locus_tag="QBF46_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ6676053.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDAAQALCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPGFIKEFEVSGNLAGFSGDYLKG
                     ASGKMVFADPWAIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     END"
     gene            5769..12014
                     /locus_tag="QBF46_003932"
                     /pseudo
     CDS             5769..12014
                     /locus_tag="QBF46_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(12006..12140)
                     /locus_tag="QBF46_003933"
     CDS             complement(12006..12140)
                     /locus_tag="QBF46_003933"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6676054.1"
                     /translation="MRAKERLPRKHYSPEFKMELVRLALEEEGSIAALARKHDVNDKH
                     "
BASE COUNT         3189 a         3052 c         3303 g         3106 t
ORIGIN      
        1 tttgtgatcc tgattacgaa aatatctcga tatttattta aaaaacttga aatgtaaatc
       61 aaaaaaagat aaacatcttc cctaagtgac ttcttttctg ttgaaaagtt caatctaaag
      121 gaaaaaaaaa tgaaaaaaat tacagtggca ctttctacgg ttgcagcatc tgttctgatg
      181 gcaatgtctg cacaagcggc agaaatctat aacaaagacg gtaacaaact ggatctgtac
      241 ggcaaagtta atgcaaagca ttacttctct tcttcagcta gtgatgacgg ggataaaact
      301 tatgctcgta tgggtttcaa aggtgaaact cagattaatg accagttaac cggttttggt
      361 cggtgggagt acgaatttaa aggtaaccgt tctgaaactg aaggttctgg taaagataaa
      421 actcgtcttg cgtttgcagg gcttcgcttt ggcgattatg gttcgctcga ctatggtcgc
      481 aactacggtg tagcttatga tgttggcgct tggactgacg tgctgccgga gtttggtgga
      541 gatacctgga ctcagactga tgtatttatg accaagcgtg ctactggcgt cgcgacctat
      601 cgtaacaata atttcttcgg cttggttgat ggtctgaact ttgctttgca gtatcaaggt
      661 aaaaatgata gtgctgcccg cgacgaagtt gaatctaatg gtgatggctt tggtctgtca
      721 gctacttacg attatgaagg atttggcgtt gctgcaacct atgcaaaatc tgaccgtact
      781 gatgggcagg tatcctatgc taaagacagc cctctgcctc tgaatgcctc tggtaaaact
      841 gctgaagtat gggctacggg cctgaaatat gatgcaaaca acatttacct ggctacaact
      901 tattctgaaa ctcagaatat gacagtcttc ggtgatgacc atatcgcgaa caaggcgaaa
      961 aactttgaag ccgttgctca ataccagttt gatttcggcc tgcgtccgtc catcgcttac
     1021 ctacactctc gcggtgaaaa tattggtgcg ttcggtaacc aggatctggt tgaatacatc
     1081 gacgtgggcg cgacttatta ctttaacaag aatatgtcca ctttcgttga ctataagatc
     1141 aacctgaacg atgaaagtga ttttaccaaa aaatctggcg ttgcaaccga caacatcgtt
     1201 gctgttggta tgacctacca attctaattt tggtaggtaa gaatatgcgg gaagggagtg
     1261 atgtcactgc ccgcatatag gtggcaccct catgccacct ttgaagaggc aataaaattg
     1321 cctcttctca atttaacttc atgttattta ttaccttttt attaatttga aactctattg
     1381 ttggggcgct ttgttgcgcc cattttttta caccaattag gtaaagttat ttttaagtaa
     1441 tcgagcaact ttcagccctc tcaaaatgga atatcgtctt caaagtccat tggaggttcg
     1501 ttcgctattg gcgttgctct gaggtttacc gccaccgctg tattgctggt ggttttgagg
     1561 ttggtttgac tgcccccagc catttgagaa ttgtgaatcg tcgcggcgag cgccgatcat
     1621 ttgcatggtg ccgccctggc tgacgataat ttccgtcgtg taacgttcta caccggcgtc
     1681 atctgtccac ttacgggttt taagtttccc ttcgatgtag acctgagaac cttttcgtaa
     1741 atattcactc gcaatttcag caagttttcc gaacaaaatg actttatgcc attctgtttg
     1801 ctctttctgt ttgcctgttt gcttgtcgcg ccatgattca ttcgttgcga tgctgagtct
     1861 tccgactgct ccgccattta gtatatacct gatatctggg tcttgtccca gggtaccaat
     1921 caggatgact ttatttacac cgcgttgtgc cacttttctt acccaacaaa ataaattaat
     1981 tagagcaata atgtatatct ttgaaacgtg gctaacaagt gatttgcatt atcctgtgcc
     2041 ttctaaaggg atcgagtcag tcggtaatgg ctgtgaatgg gtgtttgtcc tggagcgtaa
     2101 aaaattcgct tgtgaggtct ttatgaaggg aaaaacagcc gcaggaggcg gtgcaatttg
     2161 cgctatcgcg gtgatgatta ccatcgtgat gggtaatggc aatgtgcgaa ccaaccaggc
     2221 ggggcttgag cttatcggaa gcgctgaagg ttgccgacgt gatccataca tgtgcccggc
     2281 aggggtatgg actgacggga tcggtaatac acacggggta acgccgggtg tgcgaaaaac
     2341 agaccagcaa atcgccgctg attgggaaaa gaatatcctg atcgctgaac gctgtattaa
     2401 ccagcacttc cggggcaaag acatgcccga taatgccttc agtgcaatga caagcgcggt
     2461 attcaatatg ggatgcaata gcttacggac ctactacagc aaagcgcgag gcatgcgagt
     2521 agaaacgtcc atccacaagt gggcgcagaa aggggaatgg gtgaatatgt gtaaccatct
     2581 ccctgatttc gtgaacagta acggcgtgcc cctgcgaggc ttaaagattc gccgtgaaaa
     2641 agaacgccag ctttgcctga cgggactggt caatgaataa actccggcag ctccgccgac
     2701 tttcgacaat gaagttatcg ctggcggcga tagttttcga ctcgattttc atggcggtat
     2761 atgtgctcaa tgagtcgtgg ccactggaac cgctattata tgccgggctt cggctgtgcc
     2821 tgacattttt gagcatggct gcaagattga tgcagcagaa agaaaccgct tcagattgtc
     2881 cacgccgcgc ggtgcgcaaa tatatggcac gcaggcgaaa gcgataatag ttaacgatac
     2941 ccccggcagc cgccggggtt atttttggtg gttatttgaa cggattgatt gaattattaa
     3001 acgtgatgat gcttgtctta cgcggtgcct ggacgttagc cgcttgcgga acctccttaa
     3061 ttttcttggt gacaggcaag ttgcgtgctc caactttgat cagagattcg aaaagtgtgg
     3121 caacgatttt tgcatcacca ggttctttga gtcggaatgc gtctttttgg gcggcggaga
     3181 cgaatatcgg gaggttatcc agttcgtctt gcattgctgc cagcacatcg tcgcggatac
     3241 ccgcagtttt ctccagcaaa gcgattcgcg cttcagcatc tgcgatcttg gccattgctt
     3301 cgaggtggcg gccctggctt tcgagtagtg cggtttccag ttctgccgta cgctctgtcg
     3361 cctccaccat catttccagt tcagccattt tgccgtaatg ggatataacg gcctgcactg
     3421 actcgtcgga gtacccatgc gccgccaggg actctgccag taaggattta gaatccgcgc
     3481 tttcaaacat tccggcgctg gcaggatgat ccagactgat atagttcggc gttgtcacat
     3541 aatccacacc atggaagctg gtggttacag cgattttccc ggactcacgc ccgccagtgg
     3601 cccagctcca gccaccagct cggctttcga tcatcgcggc gacaatttta cccggctctg
     3661 tgttaagaat ttcctgtgta tgggtaacga tgccgttgtc gtcaacagat atagccactg
     3721 tgcggcacgc tggaacattg tcgattacga ccgggcgacc ttccaccatg atcacgctgg
     3781 tttctggtac ttccagtttg ccggtcagct gtcggcgacc gtgaccgtaa tagccgaaaa
     3841 gctcaccaag gcgtaaacct tcctgagttt ccttgctttc aagcatggtc tttaccgcgc
     3901 ttaatacata ctgtcgcccg ttctggcgac cttttctagc attactatag agacaaaagc
     3961 ggtcagtgac cgttttcaaa acatcagtca ttctcgtttc cctctttaaa gaccgattca
     4021 aggatttgcg ccagttcctg tggcggtgtt ttgatgatgg aatccatcag gtgatcgtcg
     4081 tcctcgcttt tcgctttcag ttcgttcacc agtgcttcag agattttttc gtcaatctcc
     4141 agcacatcgc tgaacaggta acgtttgaat gcatcggaat tggcgaggac gctgttattg
     4201 ctgacggcat cgaggatttg cgtaacgatg gtggcgtagt tcgcctgcga gtcgcggtta
     4261 tcgttgtgct cttgttgcag agcggtatta acggagtgga attcgatttt gtacgggcga
     4321 tcaccttccg ggtatacctt gccgtacttg aaagcaagat gaatatcgat agcccgctga
     4381 atgaactctt ctacgccctg ctggatccat gaggcgcgca tggcggcctg aattgccgtg
     4441 cgcaggaatc caccttcacc aagcccgccg gacatttgat ctgcccaccc caggagggtg
     4501 taatcgaggc caagtgctgc cgccagctgg cgcatatagg tgagaatgtc ttcaatgccg
     4561 ttgatatcag cctggatggt ctgagtatca atagtcatct gtcccttgcc gtcgcccata
     4621 ataggcagca gggtattggt caccgtaggc atgttattcg cgccgcgtgc gcgcttttcc
     4681 atcaggtcag ctgctcgttt aagcgtctga gtaatggtgc gcgaataatc ggctgctttt
     4741 accggatcca gactattcat cgccaggccg atgattcggt caattttcga cgcattaaaa
     4801 cgcgttgcct tcagcgagcg gatcgccgaa cgcagattca tgtacggctc gtaggcgtat
     4861 tcgagcaagc tggtcccgta attctgggtt tcaatcggcg tgcgctcttc cggattatcc
     4921 agcaagctgt atgctttatg gccagtgtgc acaggcataa ggtttgactt aggccgccag
     4981 taggggattt tcatagggat aatggcccac ggatcggcga aaaccatttt ccctgacgcg
     5041 cccttcagat aatcgccgct aaatcccgcc aggtttccgc tgacctcgaa ctctttgatg
     5101 aagcccggaa gggtgtaata ggagcactca aaagacgtga tccctatgcc ttctttggcg
     5161 tatggcctga cataagccac cccaaataca gacatgataa atgcccaccc ggcgacctct
     5221 ttgttgatgg ttcgcccgat gtcgttcatc agctcgtcac acaatgcctg cgcggcgtca
     5281 tagtcactat cgtttccgtt atgtaccggc acgatagaga aggtttgtcc ggtcttctta
     5341 tcgaaagaga gcgcgtgcgt aatatggatg ttcagcgcgg tggcgatcgt gctgtaaacc
     5401 gccatctctt cgagtagcgg atagcgttgc aagcgatctt ccggcagttg aacttcatca
     5461 aagataaagc gacttccgtc caccagccca tcgccagcca tgccactatc gcccggtttg
     5521 ccgcctaaga agccggacag ttgtaccggt gcccctgcgc gagaaaacaa atacccactt
     5581 ccgccgtgca cagccagcgc ggacaggagg atgttgtccc gttctccgtt gtctttaaaa
     5641 acccccgcca gcgccttcct gaccgaggat agcgtgattt tattgtctgc caagattgca
     5701 ccttaattag aataattcgc atcgtgtttg aacggaattt aacactagtc acttgttaag
     5761 gattaccaat gaacaagcta tctatggggg tgtttcgctg ttcaagtgtc agcgaaatat
     5821 tgaaatacat tagggcaata acatctcacc gagcgccgat tagatacggc gtggaaaagg
     5881 tggaaggcaa aagctatgac cgactacgcc gggaggcgaa tcagaaggcg atagatttgc
     5941 ttaattcgct ggtggacggc gcgacactga cagatgaaca gcgccagatc ctggctgggt
     6001 acaccggtga aggcggcatt ggcgggtccg tctccgaata ttacacacca aagccgatcg
     6061 ctgaaggtgt ctgggagatc atgaagctct acggcgcgga cgtaggtaac actctggaac
     6121 catcggcggg aaccggcgtt tttaatgaga caaaaccggt tggtacggtg atgaccgcga
     6181 ctgagatcag cagtgtttcc ggtcgtataa accagttgtt acacccggaa gacagcgtac
     6241 agatttcccc gttcgaacag ctggctataa gcacgcctaa cgattcattc gaccatgttg
     6301 tgggtaacgt tccgttcggc ggtcgtgata acacacgcaa catcgataag ccttacgcag
     6361 aagaaacgga catgggttct tacttcatgc tccgcatgct ggacaagata aagccaggcg
     6421 gattcatgtg cgtgattgtg ccgccgtcca ttgtttcagg ttcaaacatg aagcggttac
     6481 gcctgcgcct atcacggaaa gctgaatttc ttggtgccca ccgcttgcct accggtactt
     6541 ttgacgcaaa cgggaccagt acggtcgttg acgtggtgct gatgcgcaaa catccggcgg
     6601 agatggctga gaaaatcccc ctggtggatg aaggcactct tgaatcggca aatgtgcttt
     6661 ggccaacgtt tatttctggc aagtggtttg aaaaggatgg ccgccggttt gttcatggca
     6721 ctcaggagaa gggattccag gggcgtattg aggttcgtgc cgacggtcag attgataacc
     6781 aggctcttaa agcgaagctg attcatcgtt tcgaaagccg tatcgactgg tctttgctcg
     6841 atatggctga accgtcaccg accgcagacg ttgttggtga aggggaaatg cgcctgatta
     6901 atggtgtatg gcaaaaatat gctggtggtc gctggattga agctgatgcc gggaaggaac
     6961 ttaagatcga tgctgccagt tatggcgcgg atagctggga ggctcttcag cgtaacctga
     7021 ctacaacaga aggccgtctc ggcatgacat ttacccagat ggcaaatgtc cgcgataagt
     7081 acaccacatc aatcagcgac gatatggtgc agctggtgga ctggattaac agccagcctg
     7141 aaaaataccg tgaacgcttg tatcgtgggg cgatgattgg ccggatgtta attgaatatc
     7201 aggatatgaa ggccgccggg catagtgctg aacaaatcga acagcagcgc ctttctctgg
     7261 tatcccgttt gcaggcagag attgaccgtt ttggtaaccc cggtcgcggt ccgatagcga
     7321 aattatcggg gagcggtgcg cgcgcctggt ttgctttccg tggtgcaatt aagctggatg
     7381 gcactatttc tgacgagctg acaggaaaac tggttacgca tgattccagc gccagttatg
     7441 actccaccag ctatcaggac accctgcgtt atctctacag tgatctcact cgcgatccaa
     7501 tccagctcga tgatttccgc cttgcgttta ccggcgaact gccagccagt gatgacgagt
     7561 tgcttaattt attggccagc acccctggta ttgcggtttc accgtatggc gggattgttc
     7621 cgttcgcccg cgccaccagc ggcgacatta acgagatagt agctccaaaa caggaattcc
     7681 ttgccacact ccccgacggt ccagtaaaga acaacgtcct taatcagctg gcagcgatcg
     7741 aagagaagcg catcaagacg ccagcagaga atatccggtt taagctcaat agccgttggt
     7801 tcgaccgttc cgtcattctg gaatttttgc aggaaaacgg ctatccggat ctgcgctatg
     7861 tgcagtcagt gcagctggaa ggcgacgaaa tggtttctga cacctatcac ggtggtgatg
     7921 gtctgttcgt cgggcaccga tacggtgtcg tccagcgcaa ggataaagaa acaggcgaga
     7981 tccgctacga gtgggaccgt aaatcaggtg aaaacgcgac cgggttcccg gcacaactgg
     8041 aaaagtatct caatggtgca cgtattggtg gcaaagatag cgcgacggcg aacggctacc
     8101 gcgagcagat ggcactgctt gaggaccagt tcaataagtg gatcaagacg cacgatcgct
     8161 acgatgagct ggttgccaaa tacaacgatg ttttcaatag caatattccg tatgaacatt
     8221 ctggcgatcc gcttgggttg aagggattaa gcggtaagcg ccagccattt gattaccaga
     8281 atagcgaggt gcgccgactg tccgaagatg ggcgcggcat cctgggcttc ggcaccgggc
     8341 tgggtaaaac cacgaccgcg ctggcgcttg aggcgttcaa ctatgagaac ggtcgctcca
     8401 cccgtactgc gtatgtagtg cctaaatcag tgctggaaaa ctggtattac gaagcaaaag
     8461 aattcctgag tgaagaggca ttcagtaact acctgttcgt cggtcttgat gtgctgatgg
     8521 atggcgatca gattcgccag gtgccggtgc tcgatgagaa cggtaaacct gttcttggta
     8581 ctgatggcac tccagttatg cgcgatgccc ttaaactggc agatgaagcc actatcacgg
     8641 cgcgtatgaa cgcgatcccg cactcaaatt accgtgcagt cgtgtttacc aaagaacaat
     8701 acgcccgcat tccgctacgt gatgacaccg tagatgagca tgcacaggat atgctttatg
     8761 acttcgttgc tgccgggcgc gtagccagcg caatgaactc cgactcccac cgcaaagagg
     8821 ccgcgcgtcg ccgggtattg tcggagtatt cagataccgg taccgaaaaa gcagagaagt
     8881 atccgtactt tgaggatatg ggcttcgaca gcgtgattgc tgacgaaggc cacaactacc
     8941 gcaatagcta taaaaatggt cgcgaagcgt cacagctggc ctatctgccc accagcgcgg
     9001 tggcgcaatc ggcgcgagat atggcaatca aaaacgcgta cctgatgaaa aagaatggcg
     9061 ggcgcgggcc ggttctcctg actgcaacgc cagtcgttaa cactccgatc gatgcataca
     9121 acatgctttc tcatgttctg ccgaaggaat actggcagaa gatggggatc tacggtcctg
     9181 atgacttcgt taaattcttc ggcaagacca ggctggaaac ggtacagaaa atcagcggtg
     9241 aagttgaaga aaaaatggcg ctggtgggct ttgaaaacct tgatgcgctg cgcggcatat
     9301 tccatcgctg gacaacgctt aaaacggcgg aagacgttaa ggataccgtg gagatcccgg
     9361 agctggacga acaccagcag gatgcaccac ttactgaaga acaactggcg gcgtatgaag
     9421 aattacgcca gcaggcggaa gcggcggcca aagccaacaa tggcgtaacg acctcggtcg
     9481 atgaagacgg agtgattgag cacgagaaag cccgtccgat cttctcaata atcagggata
     9541 tggaccgcgt atgtactgac atggacctgt actatcgccg gatcacctat cgtttcctgc
     9601 cggagtacgc cgatgcagtg cagcaactgg cggacagttt gcctaaacaa gccaccagcg
     9661 aagacgacga cagtgatgat tcaatcacgc agcaatcgca atactccctg atagataagg
     9721 gcgagtttat tcagttgcag gttccggaag cgtttgagca ggaagtgaat aagcgcctgg
     9781 ccaggtttgg cattgacgaa cagaccgtaa ctcaccccgt tacacccaaa tacgcgaagc
     9841 tgatcgccac gctgaaggag tttttcccgg aaggtaagca aatcatcttc acggacgaaa
     9901 aaacgcagca ccagaagctc aagcgcatta tctgcaatgc tcttaacctt gaaccttcaa
     9961 aggtggggat cctgaatgct cagacggttg ccgaggcagg taaaaccggt aagaaactga
    10021 aagcggttaa accgccgaaa gagttaccgg atgaaccaac agatgcacag atagcgaaat
    10081 acaacgagca aatggctctg tatgacgcct atatcgcgca gcaaaatgaa atgtcgttgg
    10141 gcggtctgga aaagattgca gccgacttcc aggagggccg gactccgatc atcatctgca
    10201 acaaaaaggc agaggtgggt atcaacctgc atcgaggaac gacggatatc caccacctga
    10261 cgttgccgtg gactccagcc agtatcgcac aacgtaacgg tcgcggtgcc cgagttggct
    10321 ccaaccgtgc aagcgttcgc gttcattact actgcggcaa ggggtctttc gatgaatacc
    10381 gactgaagac gctgaagcgt aaagcaggct ggatctccga tatcctccgt tcagataagt
    10441 cagaaatgga gaacgccgat gccaacgata tgatcgaaat gcagatgtat accgctaagg
    10501 atgatggcga acgtctggca atgatgcagg ttcaaatgga taaggcgaaa gccgcgcaac
    10561 gcgctcgcca gaaagaacag gctactatcg accttcagaa ctacatcaag gcgcagcacg
    10621 cagctggcga ggatgtggag gtacttaccg ctgaattaga gcgaaacaaa gcggaacttg
    10681 aaaagaccac cgccgaggta gctaaattca aacaggcggc aatggccaaa gcagctgata
    10741 acgcagactg gaagacccgc tggggtagcg tccatcacac agaccgtacg ttgttagcac
    10801 agtatcgcgc gtcgttgaaa agcgccattc agcgcaaggc taatatctct caagccatct
    10861 cccgctatga gaaattattg aaccgtactc agaaggccgc gacggatatc aaacgcctgc
    10921 gcccgctggt ggaggatgca ataaataaag gcattctgga tgttgatcct gatctggtta
    10981 accatgcgag tgagttcctt gttatcggcg atcgctcatg gcgtgtaggc caatactacg
    11041 attgtgccgg tgatatcgtt cgcattaagt cgctggactt cgacagccag cgcgcagacg
    11101 tggagatcat ctttaccttc aaaggcacca aatcgggtaa ctgggatgtg aagacgctgg
    11161 ataaacaggt agatgtgact cccgatgaag atgctgttat gcagaaaatc agtggtggcg
    11221 tctccatcgc cgggattaac ggcatcattt cctgtgacga tttctaccgt ttccagcagc
    11281 gcggcatgat caaaatcact gactcatacg gcgttcagac tacagagtca ggctatagca
    11341 ttgattttgt tggtacctat acggacccac tgaagcatgc ggtttacccg gatcgccgtg
    11401 acggcgcgct gaagtcgtca attgctaaat gggtgcttgg tatgatgtcg gaagggaata
    11461 accgccaggt ccgtttggca gaagtattcc tgactgaact gtttggctcc aattatagcg
    11521 atgtaatcgc gtcatacgga gatacgttat cccctgaagc aattcaggag aaaatagcgg
    11581 atgcgatcgc cagaatgccg gagaaaacaa gccagggggc tactcgtaac ggggattctg
    11641 aacttgagat caccaatgcc attttcggta ccaatgagtt ccgggcgtca gattttgagg
    11701 tcaccacagc acagtttggc cccattggca tttacagcaa taaagacgag atcaagcagg
    11761 caatggacgc agcaagcgcg cgcattgcag cagaacggaa agccaatctg aatcatgcag
    11821 tcgccgcgct gactcaatcg tgggtaacag caatcaggga ggccgccacc acagggaaaa
    11881 tcacaccggc aattgcggat gtcgtaaaca acggctctaa atttatggat gcctatcaaa
    11941 tggatgcggt gcagttgcca tcagcctatg gccaactcag ctatcgcatg acctacaacc
    12001 tggtatcaat gtttatcatt gacgtcatgt ttccgggcca gtgcggcaat actgccttct
    12061 tcttcaagag ccagcctgac cagttccatt ttgaattcag gggaatagtg tttccgggga
    12121 agtctttctt tagcgcgcat gaggtgtcca cctgcaaaat aagtgggtgc ttaagttaca
    12181 ggatcctgga ttcgggacca gacggtactc agacgacgct tacctgtgtt gttctaaggt
    12241 gagcatatca cctctgttca ggtggccaaa ttcagtaaac cacttcagta ctcaatgaat
    12301 ttcaggactt caagattcgg gtgatccggt acagtgtatt tctgatcccc tataatctga
    12361 gcaaatggtg aaagactcga aacatccttc atcattaact cagccacatc attctcccta
    12421 ctggagaaga tgcctaaaac tctttgtata cttaggaata gcgccctgaa attcatcaaa
    12481 taatccttat tggcgtttac cagatagttc cgcagcaata tatgcctgca cgcttttgta
    12541 ctgttttacc ttttcgataa acttatcagg actgtaagaa ccttttacac ggcgcgacac
    12601 gttaagtgat cctacccacg taatatggac acaggcctaa gcgaggttct
//
LOCUS       DAOFQD010000136        12456 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.136, whole genome shotgun sequence.
ACCESSION   DAOFQD010000136 DAOFQD010000000
VERSION     DAOFQD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.136"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            1110..6062
                     /locus_tag="QBF46_003934"
     CDS             1110..6062
                     /locus_tag="QBF46_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6676055.1"
                     /translation="MWDGGLQEQEVVAIEKIKAAFSDNVSKPDKPVRGGSIGEQLQSY
                     GYTGNGMFPWKGYAGFRFVEAKKEGEFDLVIVTHCNVIIVELKDWNHQPVTARGETWY
                     KGGKNMGRSPVSVTRGKKFTLDNKLKRLAERFTNKGYVPIVHFFVVMTGNADFSELPE
                     EQLHHTISLTDFLKFADRNTFNNYFRPHPGSQVLNKDFPLFDNLFLGPQTAPKALRVN
                     GFEAKDMIFEHPKKVYREYLAKSEISANSEALLRVWNFRNIPGAKAYTPEGRTEIVSR
                     EREVLQHINHQNRDLYNHCLRSLTSFQKDEVTAEYSEVYELPPGHVRFNEFIGKYGKS
                     FSDQDRLNVVKLLIAKFSDLHEMKIAHRDIADHSLWISPSKEVALSNFISAYHRPVGT
                     VGDYRKLLSVGAVEVKDMLDGGELTPFQQDVHALGLVAWHLLSGMRMSPKSLETVQES
                     MLNSQHWYSDVLLDAVAAKFTSATDFFDALKQAEPAGEALPTFDDTELDPYRHPINHS
                     RQYREDDDFLVETSDKEVYISGGRLVKAWLNVGGQGDDSSINFQVLKFLKQIEQLSLV
                     KPTYLPEIREFGIASKSSSLYMVTDQIQGDTWDKMVVPDDEKIDLIDKFVASVEHLHG
                     LGVSHGDIHPGNVMLRTEGRSLFLIDIPDFSPSGDEPKNHSYSPEYIDNCTSFERDNY
                     AVMKMSCELLGLSWGDESDLYPTIANAIRAELEDPIFGFKDLGRFKKAVDSYDQASTQ
                     DLIEITAGNADEIISVLPDNGHLYVKVEPNRKEPAEVKVTFTGIGGSFSAMFSKEQKT
                     LLYGFNPRARSSIRKQDIDESQFEIGTGIRITPGSPQDMSALTVLLNKEESFARAIEL
                     ITASEDIQSQDPLTLQLKDAFARLDKRVLEPALKEVLEIPTVKLWRAILDTETESYPN
                     IEVSGEVIPVADAHGELLIPYSADVDPLGAFRSSDDVEALQVDQEGVERFIGEVSLKK
                     SELKEIRLVRVRSAAYRLKDSDIVFFRTKQDRASYQKRKRALERLIDRESVLPDLIDL
                     FDPSCKQDAQNYGVTVSDADFARYDREDQHGNKISLNEQQRKAFNILVNNGPLSLLQG
                     PPGTGKTEFIAAFVHYLIEKQNTKRILLVSQSHEAVNTAAERIRKHCSRLGTELDIVR
                     FSNREGAVSPGLKDVYSNAITTEKRELFNAEIKYRVEALSEAIGLEPGFISGVVLAEL
                     RLFKQIDHLEKLLYQVNDLTDPKEINELKDIAVELNFSIRSRLSQEYGINLDKDAKVS
                     AAKDILISRLCTEYGVRPDEARRVKALAKISRDMQDAMSGERVNLDEFYSRSRQLVAG
                     TCVGIGQGHIGIQENIYDWVIIDEAARSIASELAIAMQSARRVLLVGDHLQLPPLYSD
                     AHKAALARKLGINNSRTEIDEVLRSDFARAFNSTYGAQASAALLTQYRMAPPIGELVS
                     KTFYEGKLRNGERAIPDVYQHAPEALRNVVTWLDTASLGHRAHHLGDRGTSIYNRSEA
                     DEIISVLKQVSENEEFVAQLSKLVNKDEAAIGVICMYAEQKRLLRQKFNQEIWSDGFK
                     DIVKIDTVDSYQGKENRIIILSLTRSDKQHSPGFLRAPNRVNVAMSRAMDRLLIVGNA
                     DIWKGNNKELPLGSVVRYMTERGQDAGYVFLSAQQGGKKK"
     gene            6059..7486
                     /locus_tag="QBF46_003935"
     CDS             6059..7486
                     /locus_tag="QBF46_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676056.1"
                     /translation="MMSDNKVTYHEVDFLLPAQKFNIQFSYISQKGLPFIREFVLRLV
                     HVASMSKAQISTYFGLSHCETEEAISDLVQRGELTLSAEGRLTLTDKSNGYFSEVGEM
                     PHLSTVQDSGGTLSFDLATFSCFSNQNIQDHWRGGILLKVDDNNVSQSEKLVEKHFQY
                     QFNQILDKGYLSHLQVQGGKEQPSIYTVNSVNKLRQMPLRLTTEFKMDNDGKAVERED
                     YEHLNSSECVHELMAIEISRLLRPNNTMSIVKSMVSLNDENTLKLFDSKTNQLNPAFI
                     KDMQALEEYSASGRTTFLGPIYAKNNWERLLRALAPVLSQRIKDKADHGGSHFIWIAP
                     SDPYWSKSERFISSLSDFIHKSSTKDKRLYKPVLYVPVSDDVDSRAARQWKHELGPFH
                     EHTKGLVEGLLDGNVEILHLEDELSVVIYHISQPETLPVTMPLGFITTNKESVKIIGS
                     LITEYVHSSSGFDKPHDCGLISSMV"
     gene            complement(7653..8762)
                     /locus_tag="QBF46_003936"
     CDS             complement(7653..8762)
                     /locus_tag="QBF46_003936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676057.1"
                     /translation="MNIPLDESLQSFLLRQQLVFADVFDPKGVVSGDGQWFSKPYAHH
                     EVSYVFHRFDDLFLLETSDIGKKIGRMGHGLFDTPSAYCHNLPSIFLSGERSITYKSD
                     KKIRIKFCQKCITQAIIDFGFGYFKHDWIQARRCHEHNTPLYRLQLTSYNNALINIKQ
                     VMNGELPRNAVEIVDSYQRNNPTFAEHSISRPEFIFPIRAMKCVGEVIGVIILNNIRK
                     LPSFDGLFDIKAWLLLGEDLLLWGKDNLRFMKATLAVENAIVLLSKCDDFNYELQQAV
                     EYVKVIISPRKQFSEIIMVPKFRYCASCLECRTCAAVGEDTENIRDKVDLHFLFQHST
                     SFKRYVFDNNIILLTGNHVWSPFRVKRDILNEKSA"
     gene            complement(8874..10262)
                     /locus_tag="QBF46_003937"
     CDS             complement(8874..10262)
                     /locus_tag="QBF46_003937"
                     /inference="COORDINATES: protein
                     motif:HMM:NF018259.1,HMM:NF027308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDQ6676058.1"
                     /translation="MRLPSTLPDETLYSRLVRSLTFIGLPRSMFLTTMFGNDRASIHP
                     WLTSNLSILAENSGEDAQILFREQTLFPLFSFFLPHYCKRIYCDALALHSASRACQLT
                     NFREYERITLKYCPMCARENMEMYGVTYWHLGHQIPGIEACYLHKIWLYHIPMPPRPH
                     IQVELLPSLESHDRHCNFQAYAFANYSVSKLKAITKGEAEFTDYFRGFKNIGYITDAG
                     RVRRKSIATALYRLASQITEMNNGLLPKSDDDYSYFSNLLKGKSDQHPFKHLLIGFFL
                     EHVDLFCKGKIAELEYKKPDSSASSDKCKKLLSQGLSMAEASRRTGKSRCFIKALAIR
                     SNISLNLRPRKITVSVKENILLLAQKGFHRKEIARRWGVSVGSVETLISSVPGLVNYR
                     KAARIESKCRRHKIRIIRWLSEHPQAIRQQCRKECGAAFFWLYRHKPTWIENILPVAT
                     SPVQHRKVKHKL"
     gene            complement(10650..11222)
                     /locus_tag="QBF46_003938"
     CDS             complement(10650..11222)
                     /locus_tag="QBF46_003938"
                     /inference="COORDINATES: protein motif:HMM:NF025056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDQ6676059.1"
                     /translation="MAIIRKSEILRRYNATQTTLLEEASERRWAANSLQKAFESFSVS
                     EHYDIFLSHAYSDARIVMQIRSMLIEKGYSVYVDWIEDEHLDRGKVSEFTATVLRNRM
                     NNCSSLIYLTSNSAEKSVWMPWELGYMDARTGRVSVAPIMENDDEEFDGREYLGLYPY
                     LDLTLESFYIHSGAGSWVTFGDWMQGKKPK"
     gene            complement(11486..12055)
                     /locus_tag="QBF46_003939"
     CDS             complement(11486..12055)
                     /locus_tag="QBF46_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017898977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676060.1"
                     /translation="MAAIFLSASIPVIGRGQYYETADPFLIQCAVRELTIATVRRHKI
                     VWGGHPAITPMIWSICEDLGVDYTNSVILYQSRFFEGRYPEENQRFDNVVYTDPVPGD
                     IQGSLQLMREQMLSRKDLIGAVFIGGMDGVEVEYNIFRRFHPGAFILPVAAPGGAALN
                     LARDNGYFSGRELEDIDFATLFSRYLVDQ"
     gene            complement(12134..>12456)
                     /locus_tag="QBF46_003940"
     CDS             complement(12134..>12456)
                     /locus_tag="QBF46_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDQ6676061.1"
                     /translation="KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAE
                     SFFQLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSSDQMSPTEYENQYYQR
                     LGSV"
BASE COUNT         3685 a         2586 c         2634 g         3551 t
ORIGIN      
        1 catgtgaaat gacaataaag tttgcccgta agcctacaat taaacaataa agtttgcccg
       61 taaggacaag tagttatgct atagaacctt ctatcacata gttgcaacac ttctgttaat
      121 tgtgatgcac acaaaagtgc cgcatactcg ttatatcggt aacctactga gtatggtgta
      181 cgctatgact aaaaaatgtc caaaaacatc ccatgttggg tcaaattcca ggttgcatca
      241 tcatgtccat gggaaggaag gcatccagac tgtagtacaa gtacaggatg actccgttga
      301 aaaagaaata aggaccaagg caacaaacga cctgagaatt gagtatagtt ttggcttaca
      361 aagactcaat agcatcgctg aagaaaatgg tttgttatca gatgattacc gaacattctg
      421 acatatgatc caatagttag aaaatttcat tatgatatac taaccttagc tatccccagg
      481 ctgtagcgcc cttccgagag gtgttggccc ccttcttctg tttcgcactt tctttcttgt
      541 ttgcggctcg cctagactat ccatcgtagt cgttctcagc cccggatttt gttgtcctga
      601 acaaaggcac gaagcctacc caacgagacc gtatttgttt ctgggccgaa tcagaattga
      661 taattgtcaa cgaatacttt ttttatcata gtgttgtagt tattgttatt gcgatggcgg
      721 tatgttgacg taatttttac gggataggac acgctacaag ggagtgctca ctcggcgtac
      781 atttacgtag ttagggctga cggaacacga accagatgtg taaatcgctt ttaggacata
      841 tcgataacac attgccatca ttttattaac tgtacgagtt tgacttagta ttgaaggcat
      901 ccaaatttct gataggaagc tctgacctat agattgaatc tcactagtga gttagccgac
      961 atttcagtca attgattatg gcaaaaagct cttaattacg gaaaacatat aggaaattca
     1021 gacatattat ccgttactta caagaataac agcctaatta tctgaacaaa ttagatagaa
     1081 atctaagggg aattccgttg gaagtagaga tgtgggatgg tggcttgcaa gagcaagaag
     1141 tggtagcaat cgagaaaata aaggctgctt tttcagataa cgtatctaag cctgacaaac
     1201 ctgtacgtgg aggctcaatt ggcgagcagc tacaaagcta tggctatact ggcaacggaa
     1261 tgtttccctg gaaaggttat gcaggttttc gttttgttga ggcaaaaaaa gaaggtgaat
     1321 tcgatttagt cattgttacg cattgtaatg tgatcattgt tgaattgaag gactggaatc
     1381 atcagcctgt aacagcacgc ggagagacct ggtataaggg cggcaaaaac atggggcgct
     1441 cgccagtaag cgtaacccgt ggaaaaaagt tcacgctgga caacaaactc aagcgtctgg
     1501 cagagcgttt tacgaacaaa ggctatgttc caattgtaca tttctttgtt gtgatgactg
     1561 gcaatgcgga cttcagtgag ttaccggaag aacaacttca ccatacaatc tcgttaacag
     1621 attttcttaa atttgctgat cgcaacacat tcaacaacta cttcagaccc catccaggtt
     1681 cccaagttct gaacaaagat tttccccttt ttgataacct tttcttagga cctcagacag
     1741 cgcccaaagc tctgagggtt aatggcttcg aagctaaaga tatgattttc gagcatccta
     1801 aaaaggtgta tcgggaatac ctagcaaaat cagagatatc ggcaaatagc gaagcattac
     1861 tcagagtatg gaatttccgg aatatcccag gggcgaaagc gtacacgccg gaaggaagaa
     1921 ctgagatcgt ttcacgtgag cgtgaagtgt tacagcacat caatcaccag aatcgggacc
     1981 tttataacca ctgcctgagg tcactaacca gttttcagaa agatgaggtg acagctgagt
     2041 acagcgaagt ctatgagctt ccgccaggtc atgttcggtt taacgaattc atcgggaagt
     2101 atggtaagtc gttttctgac caagaccgtt taaatgtagt caaactactc attgcaaagt
     2161 ttagcgatct ccatgagatg aaaattgctc acagagatat cgccgatcac agcctgtgga
     2221 tttcaccttc aaaagaagtc gccctgtcaa actttatttc ggcataccat cgaccagttg
     2281 gaacggtcgg agattatcgt aagcttttat cagtgggtgc tgtcgaggta aaagacatgc
     2341 ttgatggggg cgaacttacg ccttttcagc aggatgttca tgccctgggt cttgtagcct
     2401 ggcacttatt aagtggtatg cgaatgtcgc caaagagtct ggagactgta caggagagta
     2461 tgttaaacag tcagcactgg tattcagatg tattactgga tgctgttgct gcaaaattta
     2521 cttccgcgac ggatttcttt gatgccctta aacaagctga acctgctggc gaggcgctcc
     2581 caacatttga tgatactgag cttgaccctt acaggcatcc tattaaccac tctcgtcagt
     2641 atcgggaaga tgatgatttt ctggtagaaa catctgataa agaagtctat atctcgggtg
     2701 gaaggctcgt gaaggcgtgg ctaaatgttg gtgggcaagg agacgattca tcaataaact
     2761 tccaggtttt gaagttttta aaacagatcg aacagctttc tttagttaaa ccaacgtatc
     2821 tgcctgaaat ccgtgaattt ggtatcgcat ctaaatcatc gagtctttac atggtcactg
     2881 accagataca aggagatacc tgggacaaaa tggtggtccc ggatgatgaa aaaatcgatc
     2941 tcattgataa gtttgtcgct tcagttgagc atttacacgg tcttggtgtt tctcacggag
     3001 acatccaccc aggcaatgtg atgcttagga cggaggggcg ctcgcttttc ctcatcgata
     3061 tccccgactt ttcaccgtcg ggtgacgaac ctaaaaacca cagctacagc cctgaatata
     3121 ttgataattg cacttccttt gaacgcgata actatgcagt gatgaagatg tcctgcgagt
     3181 tgttaggttt gtcctggggg gatgaatcag acctttaccc aacgattgcg aatgccatcc
     3241 gggctgaact ggaagatcct atttttgggt ttaaagattt aggccggttt aaaaaagcgg
     3301 tagacagtta tgaccaagcg tcaacacaag atctgataga gattacagca ggaaacgccg
     3361 atgaaataat ctctgttctg cctgataacg ggcatctgta tgtcaaagtt gagccaaatc
     3421 gtaaagagcc agctgaagta aaagtcactt tcaccggaat tggtggcagt ttttctgcaa
     3481 tgttcagcaa agaacagaag acgctgcttt atggctttaa tccaagggct cgttcatcaa
     3541 ttcgtaagca agatattgat gaaagccagt tcgaaattgg tacgggtatc aggattactc
     3601 caggcagccc gcaggatatg tcggcgttga cagtattgct taataaagaa gaatcttttg
     3661 cccgggcaat cgagttgatt accgcatctg aagacattca gtcgcaggac ccgcttactc
     3721 ttcagttaaa agatgccttt gccagactgg ataagcgggt gcttgagcct gctttaaaag
     3781 aagtgctgga aatcccaacg gtgaaactgt ggcgtgcaat cctggatact gaaacagaat
     3841 cctatccgaa cattgaagtc agtggggagg taatacctgt tgcagatgct cacggggagc
     3901 tactcattcc ttactctgca gacgttgatc ctcttggcgc atttagaagt agtgatgacg
     3961 ttgaagcctt gcaggtcgat caggaagggg ttgagcgatt tataggtgag gtgtcactta
     4021 agaagtcaga gctcaaggag attcgattgg ttagggtacg ttcagcagcc tataggttga
     4081 aagattctga tattgtcttc ttccgcacaa agcaggatcg ggcgtcttat cagaagcgta
     4141 aacgagcgct ggaacgcctc atcgatcggg agagtgtgct acctgactta attgacctgt
     4201 tcgacccatc ctgcaaacag gatgctcaaa attatggtgt cactgtcagc gatgccgatt
     4261 ttgccagata cgaccgtgaa gaccaacacg gaaacaagat cagtctgaat gaacagcaaa
     4321 gaaaggcgtt taacatattg gttaataatg gacctctctc cctgttgcag ggaccaccag
     4381 ggacaggtaa aactgagttt atcgcggcct ttgtccacta cctgattgag aaacagaaca
     4441 ccaaacgcat tttattagtt agtcagtcac acgaggctgt gaatacagca gccgagcgca
     4501 tcagaaagca ctgttctcga cttggtacag agctggatat cgtcaggttc agtaaccgtg
     4561 aaggggctgt ttctcctggg ctgaaagatg tctattcgaa cgcaatcaca acagagaaac
     4621 gcgaactgtt caatgctgaa attaagtacc gcgttgaagc gctgagtgaa gccattggtc
     4681 tggagccagg gtttatttcg ggtgtcgtgc ttgcggagct caggctgttc aagcaaatag
     4741 atcatcttga gaaattgctc tatcaggtta acgatttaac cgatccaaaa gagatcaacg
     4801 aactgaaaga tattgcagtc gaactcaact tttccatccg ctccagactg agtcaggagt
     4861 atgggattaa cctggacaaa gacgcaaagg tctctgcagc aaaagacatt ctgatctcaa
     4921 ggctatgtac ggagtatggt gtcagacctg atgaggctcg acgagtaaag gcattagcta
     4981 aaatatcccg ggatatgcag gatgctatgt cgggagagcg agtgaacctt gatgagtttt
     5041 attcacgttc caggcagcta gtggctggca catgcgtcgg tattggacag gggcatattg
     5101 gtatccaaga gaatatctac gattgggtga ttattgatga agctgcgcgc tcgattgcga
     5161 gtgaactggc cattgcgatg cagtcggcga gacgcgtcct gttagttgga gaccatctgc
     5221 agcttccacc attgtactca gatgctcaca aggcggcatt agcgaggaaa ttgggtatca
     5281 ataattctcg cactgaaatt gatgaggtat tacgaagtga ctttgctcgc gccttcaact
     5341 ctacttatgg tgcacaagct agtgcagctc tgctgaccca atatcgtatg gctcctccaa
     5401 ttggtgaact tgtatcaaag accttctacg aagggaagct tcgaaatgga gagcgagcaa
     5461 ttcctgatgt ctatcagcat gcaccagaag ctttacgtaa cgtagtgact tggctggata
     5521 ctgcctcctt gggtcatcgg gcccaccacc ttggagaccg tggaacaagt atttacaacc
     5581 gaagtgaagc tgacgagatt atttctgtac ttaaacaggt gtcagagaat gaagaattcg
     5641 tcgctcagct ttcaaagctc gttaataaag atgaagcagc aatcggagta atttgtatgt
     5701 atgccgaaca aaagcggttg ctacgtcaga aattcaatca ggaaatctgg agcgatggct
     5761 tcaaggatat tgttaaaatt gatacggttg acagctatca gggcaaggaa aatcgaatta
     5821 tcattttatc tctgacgcgt tcggataagc aacatagccc aggtttctta cgcgcaccaa
     5881 accgtgtcaa tgtagcaatg tcacgagcaa tggatcgctt attgatagtt ggtaatgccg
     5941 atatatggaa gggaaataat aaagagctgc cattaggctc cgttgttcgc tatatgactg
     6001 aacgaggtca ggacgctgga tatgtgttcc tctccgctca acagggaggg aaaaagaaat
     6061 gatgtctgat aacaaagtaa cctatcacga agttgacttc ttactgcctg cacagaaatt
     6121 taacattcag ttctcatata tttcccagaa aggactacca tttatccggg aatttgttct
     6181 gaggcttgtt catgtagcct caatgagcaa agcacaaata tcaacttatt tcggcttgag
     6241 ccactgtgag acggaggagg caatctcaga tcttgtacag cgcggcgaac ttacgctctc
     6301 cgcggaaggt agactgacct taactgacaa atcaaatgga tatttttctg aggttggaga
     6361 gatgccacac ctttcaactg tccaagatag tggtggtaca ttatcatttg atttagccac
     6421 ttttagttgc ttcagcaacc aaaatatcca agaccactgg agggggggga tattattaaa
     6481 ggttgatgac aacaatgttt ctcaaagtga gaaattggtt gagaaacact tccagtatca
     6541 gtttaatcag attctagata aaggctatct ttctcactta caagttcagg gcggaaaaga
     6601 acagccttct atttatacag ttaattctgt taataaactc cggcaaatgc cgttacgttt
     6661 aacaactgag tttaaaatgg ataacgatgg taaagctgtc gagcgtgaag actatgagca
     6721 cttaaatagc tctgaatgtg ttcatgagtt aatggctatt gaaatatcaa ggttattacg
     6781 acctaacaat acaatgagta ttgttaaatc catggtatca ctcaacgatg aaaatacact
     6841 taagctattt gactcaaaaa ctaatcagct taatccggcc tttattaagg atatgcaggc
     6901 cttggaagaa tactctgcct caggtagaac aactttctta ggcccaatat acgctaaaaa
     6961 taattgggaa aggctgctga gagctttggc tccagtgctc agtcagcgaa tcaaagataa
     7021 agctgatcat ggcggtagtc atttcatatg gatagcacca tcagatccat actggtcaaa
     7081 gagcgaacgc tttatctcta gcctgagtga ttttatccac aagtcgagca cgaaagataa
     7141 gcggttatat aaacctgtgc tatatgtacc cgtctcagat gatgttgatt ccagggcagc
     7201 taggcaatgg aagcatgaac taggtccgtt ccacgagcac actaaagggc ttgttgaagg
     7261 attgttagat ggtaatgtag agatccttca tctcgaggat gaattatctg ttgttatcta
     7321 tcatatttct caaccagaaa ctttgccagt gacgatgcca ttgggattta taacaactaa
     7381 caaagagtca gttaaaatta ttggatcgct aattactgaa tatgtgcata gtagttctgg
     7441 ttttgataaa ccgcatgact gtggattgat ctcttcaatg gtttagaaac tgttatttga
     7501 ttgacaatga aagggctatt tactgccctt tcatttatta ttgaacacga ttgtgttctc
     7561 ccaactcctc tttcatttca gctaaaagta taaaaattta gaataggctt ggacaaacca
     7621 aattattatg ggctaaatat tatttagcat tactatgctg acttttcatt taatatatct
     7681 cttttcaccc taaatggtga ccaaacatgg ttgccggtta gtaaaattat attattatca
     7741 aaaacatatc gcttgaatga tgtggagtgt tgaaatagaa aatgaagatc gactttgtct
     7801 ctaatatttt ctgtatcctc cccaacggca gcacaagttc gacattccaa gcaagacgca
     7861 caatatctaa atttaggaac cattataatt tcagaaaatt gttttctagg agagataatt
     7921 actttaacat actccacagc ctgttgaagc tcataattaa aatcatcaca tttactcagc
     7981 aacactatgg cattttcaac agccagagta gccttcataa accgtaaatt atcttttccc
     8041 cagagaagca aatcttcgcc taacagcaac catgccttga tatcgaataa gccatcaaaa
     8101 cttggtaatt ttcttatatt atttaaaatg ataaccccaa taacctcacc gacacacttc
     8161 attgctctaa ttggaaatat aaactcaggt cttgatatag agtgctctgc gaatgtagga
     8221 ttatttcttt gatatgaatc gacgatctca actgcattcc taggaagttc tccattcata
     8281 acctgcttaa tattaataag agcattatta tacgaagtca gctgaagacg ataaagtggt
     8341 gtattgtgtt catgacaccg tcgggcctga atccagtcat gcttaaaata accaaaccca
     8401 aaatcaataa ttgcttgcgt aatacatttt tgacagaatt ttattcttat ttttttatct
     8461 gatttatatg ttatagatcg ctctccagag agaaatatgg atgggagatt atgacaataa
     8521 gctgatggtg tatcaaatag accatgtccc atcctaccta tttttttccc gatatctgag
     8581 gtttcaagta aaaaaagatc atcaaatcta tggaagacat atgaaacttc atgatgtgca
     8641 tagggtttac taaaccattg accatcacct gatactaccc ctttagggtc aaagacatct
     8701 gcaaaaacca actgttgtct aagaaggaag ctttgcaatg attcatctaa tggaatattc
     8761 acagattttt tctcacagta tttataccta atctcattat tatttttatg tgtttatatc
     8821 agatatattc gttgaatggg tagtattatt tacacatcaa ataaaatcaa cgtctaaagt
     8881 ttatgtttca ctttcctatg ttgaactggt gatgttgcta caggtaatat attttctatc
     8941 caagtaggtt tatgtcggta aagccaaaaa aaggcagcgc cacactcttt tctacattgc
     9001 tgccgtattg cttgaggatg ctcacttaac catctaatta ttcttatttt atgacgccgg
     9061 cattttgatt caattctagc agctttccga taattgacta gaccaggaac actggaaata
     9121 agagtttcta cactaccaac agaaactccc caacgtctag ctatttcttt tcgatgaaat
     9181 cctttttgtg ccaatagcaa aatgttttcc tttaccgaaa cagttatttt ccttggtcga
     9241 agattcaacg aaatatttga ccttatagct aatgccttta taaaacatct actttttccc
     9301 gtacgtcgac tggcctcagc catcgataat ccttgagaaa gcaatttctt acatttatca
     9361 ctagaagctg atgaatctgg tttcttatat tctagttctg ctatttttcc cttgcaaaac
     9421 aaatcaacat gttctagaaa aaaaccaatc aagaggtgtt taaaaggatg ttggtcagac
     9481 ttccccttca ataaattcga gaaataacta taatcatcat ctgatttagg tagaagccca
     9541 ttattcatct ctgttatttg acttgctaat cgatacagcg cagttgcaat tgatttccta
     9601 cgtacacgtc cagcatctgt tatataacct atatttttaa atcccctgaa ataatctgtg
     9661 aattccgcct ctccttttgt tatcgcttta agctttgaca ctgaataatt tgcgaaagca
     9721 tatgcttgaa agttacaatg cctatcatgc gattcaagtg atggtaataa ttccacttga
     9781 atatgcgggc gaggtggcat tggaatatga tataaccata tcttgtgtaa ataacaggct
     9841 tcgataccgg gtatctggtg acctaagtgc caatatgtta caccatacat ttccatattt
     9901 tctctcgcac acatagggca atattttaat gtaattcttt catattctct gaaattagta
     9961 agctgacatg ctctacttgc cgaatgtaat gctaatgcat cacaatagat tcgcttacag
    10021 taatgcggta aaaaaaaact gaataaggga aagagtgtct gttctctaaa aagtatttgc
    10081 gcatcttcac cactattttc agccaatatt gaaagattcg aagtcagcca aggatgaata
    10141 cttgccctat cgttaccaaa catagtagta aggaacatac tacgaggtaa accaataaaa
    10201 gttaaactac gaactaaacg actatataaa gtttcgtctg gcaatgtaga aggaagcctc
    10261 atacctacct ccaccatagt tagaaatatg ccttacaagg taagatcacg gattttactt
    10321 ttaagataaa atgaatcgcc acggataatc tagacacttc cgagcgtctc ctccgggaac
    10381 ataatcagta tatcggaata tctctaaaag tgaatgggcc gttttgccgg gatgcttaca
    10441 agcgggcgtt tcccagaagc tgatgtacgc cacgctccgg aagtttggag cccgcatcaa
    10501 tgaagcactt gcgctcaaag agctgaaatt tattcttcat ccgccttacc tggttataga
    10561 gccaggtacc caaaggcagt gggctggaaa gcgctagttg gtgagcgccg ttgttttttt
    10621 gtgcaacggc ttgttaggca tatcatgttt tacttcggtt ttttcccttg catccaatct
    10681 ccaaacgtaa cccatgaccc ggcgccgcta tgtatataaa agctttctag agttagatct
    10741 aaataagggt acaggcctag gtattctctc ccatcaaact cttcatcatc attttccata
    10801 ataggcgcaa ctgatactct tccagttcgc gcatccatat atcctaactc ccatggcatc
    10861 caaaccgatt tttctgctga attagaagtt aaatagatta gagatgagca attattcatt
    10921 ctattacgca gtacggttgc agtaaattcg cttacttttc ctctatctaa atgctcatct
    10981 tctatccagt caacatatac ggaataacct ttttcgatta gcatgcttct aatttgcata
    11041 actattcgcg catcagaata agcatgagaa agaaaaatat cgtaatgctc agaaacggag
    11101 aaagattcaa atgctttttg caggctattt gctgcccagc gcctttcact ggcctcctct
    11161 aataatgttg tctgtgtggc attgtagcga cgaagtatct cagatttacg aattatagcc
    11221 attattcttc tcgtctaaat tttgcatcca tctacctaaa gggtaacgac atgaagctag
    11281 tatttggtga ttatacctct ctatgatttt gatttatgga tacgaattga ttattcaggc
    11341 acatatggaa aatgctttgt aacccaagcg tatccatcgt tattggcagc tccttccgag
    11401 gtatatcgca ttaacatcta tatgatgata agaaaccaaa atggccctcg cttggtttac
    11461 aaaaatacag gatcgtacaa gacaactatt gatcaactaa ataacggcta aagagcgtgg
    11521 cgaaatctat atcttcaagc tctctcccgc tgaaataacc attatcccgt gcaagattaa
    11581 gcgccgcacc gcctggcgcg gcaactggaa ggatgaatgc cccaggatgg aaccgcctaa
    11641 aaatgttgta ttcgacctca acaccatcca tccccccaat gaaaactgca ccgataagat
    11701 cttttctgga gagcatctgc tcgcgcatta gttgcaagct accttgaatg tcgccgggca
    11761 cggggtcggt ataaaccacg ttgtcaaatc gttgattctc ttccgggtag cgaccttcga
    11821 agaagcggct ttgatacagg atgacagagt tagtgtaatc aaccccaaga tcttcacaga
    11881 tgctccaaat cataggggta attgctggat gaccgcccca tacgattttg tgcctgcgaa
    11941 cggtcgcaat agtcagttca cggacagcgc actggataag aaatgggtca gcggtctcgt
    12001 aatattgccc tcgtccaatc acggggatac ttgctgagag gaaaatcgcc gccatttcta
    12061 taccctccaa tctgcaaatt gccaactcgc ctcgcaagcc ttaacccatc gcgtatgaat
    12121 cgccacggat aatctagaca cttccgagcc gttgataata ctggttttca tattctgtcg
    12181 gtgacatctg atcgctggaa ccatgccgac gcttactgtt ataaaacatt tcgatgtaat
    12241 caaaaatatc gctgcgggct tcttcccgcg ttccgtagat ctttttcttt atccgttcgc
    12301 gtttcaacaa ctggaaaaag ctttctgcaa ccgcattatc atggcaatta ccgcgacggc
    12361 tcatgctgcc ctccaggccg tgtgatttca ggaacgactg ccactcatgg cttgtgtact
    12421 gactgccctg atccgaatga accagcacct gttttt
//
LOCUS       DAOFQD010000137        12399 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.137, whole genome shotgun sequence.
ACCESSION   DAOFQD010000137 DAOFQD010000000
VERSION     DAOFQD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.137"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            310..1542
                     /gene="mdtM"
                     /locus_tag="QBF46_003941"
     CDS             310..1542
                     /gene="mdtM"
                     /locus_tag="QBF46_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDQ6676062.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRVGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPLQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNSGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            1583..2863
                     /locus_tag="QBF46_003942"
     CDS             1583..2863
                     /locus_tag="QBF46_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDQ6676063.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIEKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRIAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            2979..4130
                     /locus_tag="QBF46_003943"
     CDS             2979..4130
                     /locus_tag="QBF46_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDQ6676064.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QELPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEILRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPHPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            4140..4907
                     /locus_tag="QBF46_003944"
     CDS             4140..4907
                     /locus_tag="QBF46_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676065.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            4904..5161
                     /locus_tag="QBF46_003945"
     CDS             4904..5161
                     /locus_tag="QBF46_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDQ6676066.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            5226..6086
                     /locus_tag="QBF46_003946"
     CDS             5226..6086
                     /locus_tag="QBF46_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDQ6676067.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            6154..7332
                     /locus_tag="QBF46_003947"
     CDS             6154..7332
                     /locus_tag="QBF46_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6676068.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWWALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(7345..7899)
                     /gene="kptA"
                     /locus_tag="QBF46_003948"
     CDS             complement(7345..7899)
                     /gene="kptA"
                     /locus_tag="QBF46_003948"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDQ6676069.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            8149..8832
                     /locus_tag="QBF46_003949"
     CDS             8149..8832
                     /locus_tag="QBF46_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676070.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            8829..9290
                     /locus_tag="QBF46_003950"
     CDS             8829..9290
                     /locus_tag="QBF46_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDQ6676071.1"
                     /translation="MTTQVRKNIMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            9303..10475
                     /gene="iadA"
                     /locus_tag="QBF46_003951"
     CDS             9303..10475
                     /gene="iadA"
                     /locus_tag="QBF46_003951"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDQ6676072.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            10540..11451
                     /gene="hypT"
                     /locus_tag="QBF46_003952"
                     /gene_synonym="qseD"
     CDS             10540..11451
                     /gene="hypT"
                     /locus_tag="QBF46_003952"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDQ6676073.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVMPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(11444..11836)
                     /gene="iraD"
                     /locus_tag="QBF46_003953"
     CDS             complement(11444..11836)
                     /gene="iraD"
                     /locus_tag="QBF46_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDQ6676074.1"
                     /translation="MMRQSLQAVLPEISGNKTSPLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDESLTFDLVWDNGGWRSATLENVS"
     gene            complement(11833..11916)
                     /gene="idlP"
                     /locus_tag="QBF46_003954"
     CDS             complement(11833..11916)
                     /gene="idlP"
                     /locus_tag="QBF46_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDQ6676075.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2841 a         3110 c         3175 g         3273 t
ORIGIN      
        1 caccttaaaa tcaccatcat acactaaatc agtaagttgg cagcattacc aaattttaat
       61 tacacttaag gtgtatattt tctatgcaag ccatcaatta aagaggtgta atgtgctgat
      121 gactatttgt aatcgttata cattctgacc tggagtcaga aggtatttct ctgtctgtgt
      181 attcgcaggc agtgtggttg attacatgaa attcagtaca tttgtccttc tcgcctgcct
      241 ttcgtcatta ccgacggtat tgaatttcgt tttccccatt ggggttctcc ggacaaggag
      301 ttgtttgtta tgccacgttt ttttgcccgc catgccgcca cgctgttttt cccgatggcg
      361 ttgattttgt atgactttgc tgcgtatctg tcgacggacc tgatccagcc agggatcatt
      421 aatgtggtcc gtgattttaa tgccgatgtc agtctggccc cggctgccgt cagtctctat
      481 ctcgctggag gcatggcatt gcagtggctg ctggggccgc tttccgacag ggttggccgc
      541 aggccggtgc tgattaccgg ggcgctaata tttacccttg cctgcgctgc gacaatgttc
      601 acaacgtcta tgacacagtt tcttatcgcg cgtgcaattc agggcaccag tatctgtttt
      661 attgccaccg ttggttatgt cacggtgcag gaggcgttcg gacagacaaa agggatcaag
      721 ttgatggcga ttatcacctc catcgtactg attgcgccga ttattggccc gctttccggc
      781 gcagctctga tgcactttgt gcactggaaa gtcctttttg ccatcattgc ggttatgggt
      841 tttatctcat ttgttggctt actgctggcg atgccagaga cggtgaagcg cggcgcggtt
      901 ccatttagcg ccaaaagcgt cttgcgcgat tttcgtaatg tcttttgcaa tcggctgttc
      961 ctctttggcg cagcaaccat ctctttaagc tatatcccca tgatgagttg ggtggctgtc
     1021 tcgccggtga tcctgatcga cgcgggcggc ttaacaactt cgcagttcgc ctggacacag
     1081 gttccggtgt tcggcgcggt gattgtcgcg aatgccatcg tggcgcgttt cgttaaagat
     1141 ccgaccgaac cgcgctttat ctggcgtgcc gtaccccttc aactggttgg cctcgcgctg
     1201 ttgattatcg gcaatctgct gtcaccgcac gtctggctgt ggtcggtgct gggcaccagt
     1261 ctgtatgctt tcgggattgg tttgattttc ccgaccttat tccgctttac gctgttttcc
     1321 aataacttac cgaaagggac cgtctccgca tcgctgaata tggtgatcct gatggtgatg
     1381 tctgtctcgg tcgaaatcgg ccgctggctg tggtttaaca gcggtcgctt gccgtttcat
     1441 ctgttagccg ttgtggcagg cgttatcgtc gttttcaccc tggccggatt gcttaatcgc
     1501 gtgcgccaac atcaggctgc cgagctggcg gaggagcggt gatttttgcg cgatcctgcc
     1561 gtcaggctct attcttaaca ttatgaataa actcattgaa ctcagacgcg ccaaaatgtt
     1621 ggcgctctct ttactgctta tcgccgctgc tacctttgtc gttacgctgt ttttgccgcc
     1681 caatttttgg gtgagcggcg tgaaggcgat tgctgaagcg gcgatggtcg gcgcgctggc
     1741 ggactggttt gcggtggtgg cgctgtttcg ccgcgtgccg attccgatca tttctcgtca
     1801 tacggcgatt attccgcgta ataaagaccg gattggcgaa aatctcggcc agttcgtgca
     1861 ggaaaaattt ctcgataccc aatcgctggt ggcattgatt cgacgtcacg aaccggcgtt
     1921 gttgattggc aactggttta gtcagccaga aaacgcccgc cgcgttggtc agcatctgtt
     1981 gcagatcatg agcggttttc ttgaactaac cgatgatgcg cgtattcagc gcctgcttaa
     2041 gcgcgcggtc caccgggcga ttgataaagt cgatctttct ggcaccagtg cgttgatgct
     2101 ggagagtatg actaaaaacg atcgtcatca ggtgctgctg gatacgctga tcgcacagtt
     2161 gatcgccctt ctccagcgcg ataaatcgcg caagtttatt gcccagcaaa ttgttcgctg
     2221 gctggagagc gagcatccac tgaaagccaa aattttgccc actgaatggc tgggcgaaca
     2281 tagcgcggag ttggtttctg acgcggtgaa ttctttgctt gatgatatca gccgcgatcg
     2341 tgcgcatcag atccgccatg cgtttgatcg cgctaccttc gccctgatcg agaaactgaa
     2401 aaacgatccg gaaatggcgg cgcgagccga tgccgtaaaa agctatctga aagaagatga
     2461 agcttttaac cgctatctca gtgaattgtg gggggattta cgggaatggc tgaaagcgga
     2521 tatcaacagt gaagattctc gtgtgaaaga acgtatcgcg cgggctggtc aatggtttgg
     2581 cgaaacgtta attgccgatg atgccttgcg ggcgtcgtta aatggtcatc tggaacaagc
     2641 cgcacaccgc attgcgcctg agttttccgc attcctgact cgccatatca gcgacacggt
     2701 aaaaagctgg gacgcacggg acatgtcgcg gcaaatcgag ttaaatatcg gcaaagatct
     2761 ccagtttatc cgtgtcaacg gtacgctggt tggcggttgt attggactta ttttgtattt
     2821 gctgtcgcag ctcccggcct tgttccccct cggcaattta tagaaatcca tgaaaaacta
     2881 agtggctaac cgcgagagag atcaaataaa ccacactaaa agaatggtaa taatgtggcc
     2941 tcttttcatt atctcccgtg gtacggggaa ggaaaatcat gtcacttgtc accgatctac
     3001 ccgccatttt cgatcagttc tctgaagctc gccagaaagg ctttctcacc gtcatggatc
     3061 tcaaggagcg cggcattccg ctggttggca cctactgcac ctttatgccg caagagctcc
     3121 cgatggcagc cggtgcggtt gtggtttcgc tatgttccac ctctgatgaa accattgaag
     3181 aagccgagaa agatctgccg cgcaacctct gcccgctgat taaaagcagc tacggcttcg
     3241 gcaaaaccga taaatgcccc tacttctact tttctgatct ggtggtcggt gaaaccacct
     3301 gcgacggcaa aaagaaaatg tatgaataca tggcggagtt taagcccgtt catgtgatgc
     3361 aactgccaaa cagcgttaag gacgatgcct cgcgtgcatt atggaaagcc gagatactgc
     3421 gcttgcaaaa aacggtagaa gaacgttttg ggcacgagat tagcgaagat gctctgcgcg
     3481 atgccattgc gctgaaaaac cgcgaacgtc gcgcactggc aaacttttat catttggggc
     3541 agttaaatcc tccagcgctt agcggcagcg acattctgaa agtagtctac ggcgcaacct
     3601 tccggttcga taaagaggcg ttgatcaatg aactggatgc aatgaccgcc cgcgttcgtc
     3661 agcagtggga agaaggccag cgactggacc cgcacccacg tattttaatc accggctgcc
     3721 caattggcgg cgcagcagag aaagtggtgc gcgcgattga agagaatggc ggctgggttg
     3781 tcggttatga aaactgcacc ggggcgaaag ccaccgagca atgcgtggcg gaaacgggtg
     3841 atgtgtacga cgcgctggcg gataaatatc tggcgattgg ctgctcctgt gtttcgccga
     3901 acgatcagcg cctgcaaatg ctcagccaga tggttgaaga atatcaggtc gatggcgtag
     3961 ttgatgtgat tttgcaggcg tgccatacct acgcggtgga atcgctggcg attaaacgtc
     4021 atgtgcgcca gcagcacaac attccttata tcgctattga aacagactac tccacctcgg
     4081 atgtcgggca gctcagcacc cgtgtcgcgg cctttattga gatgctgtaa ggagtggcag
     4141 tgacatattc gattggcatt gattccggct caaccgccac caaagggatc ttactggctg
     4201 acggcgtgat tacgcgccgt ttcctcgttc caacaccctt tcgcccggca acagcaatta
     4261 ctgaagcctg ggaaactctg cgcgaagggt tagagacaac gccgtttctg acgctcaccg
     4321 gctacggacg gcaactggtg gattttgccg ataaacaagt gacggaaatc tcctgtcacg
     4381 ggctgggcgc acggtttctt gagccagcaa cgcgcgcggt aatcgacatc ggtggtcagg
     4441 acagcaaagt gattcagctt gatgatgacg gtaacctgtg cgatttcctg atgaatgaca
     4501 aatgcgcggc gggcaccggg cgtttcctgg aggtgatctc gcgcacgctt ggcaccagcg
     4561 tcgagcaact cgacagcatt accgaaaatg tcataccgca cgccatcacg agtatgtgca
     4621 cagtgtttgc tgaatcagaa gtgatcagcc tgcgctctgc gggcgtagcg ccagaagcga
     4681 ttctcgccgg agtgattaac gcgatggcgc ggcggagcgc caatttcatt gctcgtctct
     4741 cctgtgaagc gccgattctg tttactggtg gcgttagcca ctgtcaaagg tttacccaca
     4801 tgctggaaag ccatctggga atgccggtac aaactcaccc tgatgcgcaa ttcgctggtg
     4861 caattggcgc ggcggtgatc ggtcagcggc agagaaaacg cgcatgaaag agtatctgtt
     4921 tttatttcac tccacggtcg gcgtcataca aacccgcaag gcgttgcagg cagcgggcat
     4981 gacttttcgc gtcagcgata ttccccgtga tttacgcggc ggctgcgggt tatgcatttg
     5041 gctgacctgt ccgcctggcg aggaaattca atgggtgatc cctgggcata cggaatcagt
     5101 ttattgtcag caggatggtg gctggcgctg cattgcacat tacgggattt ctccgcgtta
     5161 aggattcatt aagatcgcta cgtatacttc tcgatatctt attgatttgc cttaaaggga
     5221 ttaccgtgac aaaaagtatt tcgttaagta aacgcatctt cgttattgtg attctttttg
     5281 tcattgtggc agtgtgtact ttcttcgtgc aatcttgcgc ccgtaaaagc aatcatgcag
     5341 catcatttca gaattaccac gccaccattg acggcaaaga aattgcaggc ataacgaata
     5401 atatctcttc gctgacctgg tcggcacaaa gcaatactct ttttagcact atcaacaaac
     5461 ctgccgccat tgttgaaatg accaccaatg gcgatttgat ccgcacaatt cctctggatt
     5521 ttgttaagga tctcgagacg atcgaataca ttggcgataa tcagtttgtc attagcgatg
     5581 agcgcgatta tgccatttat gtgatttcac tgaccccaaa ctcggaagta aaaatcctca
     5641 aaaaaataaa aatcccgctg caggaatccc caaccaattg cggttttgaa ggattggcct
     5701 attctcgcca ggatcacact ttctggtttt ttaaagagaa aaacccgatt gaggtctaca
     5761 aagttaacgg gctgttaagt agcaatgaat tgcacatcag caaagacaag gcactacaac
     5821 ggcaatttac tttagatgat gtgtccggcg cagaatttaa ccaacaaaaa aatacgcttc
     5881 tggtgctgtc tcatgaatct cgcgcactcc aggaagtaac gctggctggc gaggttatag
     5941 gtgaaatgtc actaaccaaa ggcagtcgag gtttgtcgca taacatcaaa caggccgaag
     6001 gggttgcgat ggatgcctca ggtaatattt atatcgttag cgaaccaaat cgtttttatc
     6061 gctttacccc tcaatcatcg cattaacagg ctggcatgtt tcaccttttt gttattaact
     6121 tgttaacaac aaaaatgaca aacaaggtta aacatgcctt cgccctcgca tcccgtagaa
     6181 cgcttttctt tcagcaccgc gcttttcggg atgctggttc tgaccttagg tatgggttta
     6241 ggccgcttcc tttatacgcc tatgttgccc gtcatgatgg cggaaggatc gttttcattt
     6301 agccagctct cgtggattgc cagcggcaac tatgcggggt atctggctgg cagtctgcta
     6361 ttttcgtttg gcgcatttca ccagccatcg cgcctgcgcc catttctgtt ggcttctgcc
     6421 ctggcgagcg ggttgttgat cctcgcaatg gcatggttgc tgccatttat tctggtgtta
     6481 ttgattcgcg tcctggcggg tgtcgccagc gccggtatgc tgatttttgg ttcgacgctg
     6541 attatgcagc acacgcgcca tccttttgtg ctggcagctt tgttttctgg cgttggcatt
     6601 ggcatcgcac tgggcaatga atatgttctg gcaggcctgc attttgacct ctcctcgcaa
     6661 acgttatggc aaggcgccgg ggcgctttct ggcatgatgc tgattgcact tacgctttta
     6721 atgccctcga aaaaacacgc catcacacca atgccattgg caaaaacgga gcaacagata
     6781 atgagctggt ggttactggc tattctgtac ggcctggcgg gatttggtta tatcatcgtc
     6841 gcgacttacc tgccgctcat ggcgaaagat gcaggctcac cattgttaac cgcccatctc
     6901 tggacgttag tcggcttatc aattgtgcct ggttgctttg gctggctatg ggcagcaaaa
     6961 cgttggtggg ctttgccctg cctgacggcg aatttgctgg tgcaggctat ctgtgtgctg
     7021 cttactctcg ccagcgactc gcctctcttg cttatcatca gcagtcttgg ttttggcggt
     7081 acctttatgg gcacgacatc tctggtgatg actatcgccc gccaactgag cgtaccggga
     7141 aatctcaatc ttttaggctt tgtgacgctc atttacggca ttggacaaat cctcggcccg
     7201 gcgctgacca gtatgctcgg caatggaaca tcggcgcttg ccggcgccac tctctgcggc
     7261 gcggcggcgt tgtttattgc tgcgcttatc agcacagtac aattatttaa gctgcaagtg
     7321 gtcacttcat gaggagtgtt tcccttacca ctcaagaaat tcgaccgcaa ccgttgatgt
     7381 cagccagacc ccgttttccg cttgccagaa agggaggcct cgtttcgcca tttcctgtgc
     7441 tttgacggtt aaaataaccg gcgatccgtg tcttgctccc actttgcgtg ccgttgcttc
     7501 atcagcagag agatgtacgt agtgacgctc acctgcaatc agcccctgtt tttttatctc
     7561 gtcaaggaaa cggcttgccg taccgtggta gagaaactgt ggcggtgttt tttcggcaaa
     7621 ggaaatagcg acctgcgaag tcgaatgccc ctgaaccgcg cggatgcacc taccatcact
     7681 ggaataacta aaacgctttt tatcgctggt ggcaaccacg gtatccagca atgcacgggt
     7741 taaccgcttg cccgcttttt gggcacaaag tatgagctta tcgatatccg cccatccttc
     7801 acggtccagt acgataccaa tcgcctccgg tttgtgtcgg aggacaaaac ttaaaaactt
     7861 gctcgtatcc gccagctctt tttcgttgta ctttgccatc gcgttttcgt ccctggatta
     7921 tctgtttttt atttactatg tcattgtcac tgggtttatg aattttctgc aactcaatcc
     7981 tgcgtagaac cttttccaca cagcgtaatt ttaccgctat gcaaaaaacg catagtcaga
     8041 aacatacttg cattttcccc atacccacat ggctgataag gtttttcagc tccaggaatt
     8101 ttcctggctc aagcaacaca caaacacaaa aaaacaatgg ggattgttat gacacaacaa
     8161 ggggatgctg tagccgggga gctggcgaca gaaaaagtgg gcattaaagg gtatctggct
     8221 ttttttctca ccataatctt tttttccggt gttttttccg ggacagacag ttggtggcgc
     8281 gtttttgatt tttccgttct gaacggatcg tttgggcaac tgcctggtgc gaacggagcc
     8341 accacctcgt ttcgcggcgc gggtggcgcg ggggctaaag atggctttct ctttgcgctg
     8401 gagctggcac cgtcagtcat tctctctctg gggattattt caattaccga tggtcttggc
     8461 ggattacgcg ccgcgcagca gttaatgacg ccagtgctaa aaccgttgtt gggcattcca
     8521 gggatctgct cgctggcgct gatcgctaac ctgcaaaaca ccgatgccgc cgcaggtatg
     8581 accaaagagc tggcgcagga aggtgaaatt accgaacgcg ataaagtgat tttcgccgca
     8641 tatcaaacca gcggtagcgc cattatcacc aactacttct cttccggcgt cgcggtgttt
     8701 gcctttctgg gcacgtccgt aatcgtacct ttagcagtca tcctggtgtt taaatttgtc
     8761 ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac gtcgcaaccc aacgcaagga
     8821 gcgcaagcat gacaactcag gtccgcaaaa acatcatgga tatgtttatc gatggtgcgc
     8881 gccgtggctt tactatcgcc acgaccaacc tgctgccaaa cgtggtgatg gcgtttgtca
     8941 tcattcaggc gctgaaaatt accggcctgc tcgactgggt ggggcatatc tgtgaacccg
     9001 taatggcgct gtggggatta cccggtgaag cggcaaccgt ccttttagcc gcattaatga
     9061 gtatgggtgg cgcagtcggt gttgccgcca gtctggcaac cgctggcgca ttaaccggtc
     9121 acgatgtcac cgttttactg cccgcgatgt atttaatggg caacccggtg caaaatgtcg
     9181 gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta cccgcatatc attacggtct
     9241 gcgtgattaa cgcattgctg tcgatctggg ttatgcagct tattgtttaa caaggagtta
     9301 ccatgattga ttataccgca gccggtttta ccctgctgca gggagcgcat ttgtatgcgc
     9361 cggaagatcg gggaatttgc gatgtcctcg tcgctaacgg caaaattatc gccgttgcca
     9421 gtaatatccc ttctgacatt gtaccggact gcacggttgt cgatctcagc gggcagatcc
     9481 tctgcccagg ttttattgat caacacgtcc atttgattgg cggtggcggc gaagcaggtc
     9541 ccacgacgcg cacgccggaa gtggcgttaa gtcgtctgac ggaagcaggc gtcacgtcag
     9601 tggttggtct gctgggcacc gactctatct ctcgccaccc ggaatccctg ctcgccaaga
     9661 cccgtgcgct caatgaagaa ggcatcagcg cctggatgct gaccggcgct tatcatgtcc
     9721 cttcccgcac tattacgggt tccgtggaaa aagacgtggc gattatcgat cgcgtgattg
     9781 gcgtgaaatg cgccatctct gatcaccgtt ctgccgcacc ggacgtttat cacctggcta
     9841 atatggcggc ggaatcccgc gttggcggtt tgctcggcgg taaacctggc gtcaccgtgt
     9901 tccacatggg cgacagcaaa aaggcgttac agcctgtcta tgacctgctg gaaaactgcg
     9961 atgtgccaat tagcaagttg ctgccgaccc acgttaaccg caacgttccg ttgtttgagc
    10021 aggcgctgga gttcgcgcgc aaaggcggca ccatcgatat caccagcagc attgacgaac
    10081 cagtcgcccc tgccgaaggt attgcccgcg ccgttcaggc gggtattccg ctggcacgcg
    10141 tcaccctcag ctccgacggc aacggtagcc agccgttctt cgatgacgaa gggaatttaa
    10201 cccatatcgg tgttgccggt tttgaaacgt tgctggaaac cgtgcaggtg ctggtcaaag
    10261 attatgattt cagtatcagc gatgccctgc gcccgctcac cagtagcgta gccggtttcc
    10321 ttaacctgag cgggaaaggc gaaattctgc caggcaatga cgcagactta ctggtcatga
    10381 cgccagaatt acgcattgag caggtatacg ctcgcggcaa gctgatggtc aaagacggca
    10441 aagcctgcgt gaaaggaacg tttgaaacgg cataaacggt tgtcagccgc ccgaatggac
    10501 gtagaatgcc cgcaagggcg gctgacagag taaaacgtaa tggatgactg tggtgcggtt
    10561 ttgcataata ttgaaactaa atggctttat gattttctga ccctggaaaa atgccgcaat
    10621 ttttcccagg cagcagtcag tcgcaacgtc tcgcaaccgg cattcagccg ccgcatccgt
    10681 gcgctggaac aggcgattgg cgttgaattg tttaaccgcc aggtgatgcc gctgcaactc
    10741 tcggaacaag gtaaaatatt ccattcgcag atccgccatc tgttgcaaca gttagagagc
    10801 aaccttgcag aactccgtgg tggcagcgat tacgcgcaac gcaaaattaa aatagccgct
    10861 gcacactctc tttccctcgg gctgttaccg tccattatca gccagatgcc gccgctcttt
    10921 acctgggcaa ttgaagctat tgatgtcgat gaagcggtcg ataaactgcg tgaagggcaa
    10981 agtgactgta ttttttcctt tcacgacgaa gatttgctgg aagcgccgtt tgaccacatt
    11041 cgcttatttg aatctcaatt gttccccgtt tgcgccagtg acgaacacgg agaagcactt
    11101 tttaacctcg cgcagccaca ctttccgtta ctgaattaca gccgcaactc ctacatgggg
    11161 cgattgatta atcgcaccct gacgcgccac agtgagttaa gtttcagcac cttttttgtc
    11221 tcttcgatga gcgagctttt aaagcaggtt gccctcgacg gctgtgggat tgcctggcta
    11281 ccggaatacg ccatccaaca agaaattcgc agcgggcagc tcgttgtact gaatcgggac
    11341 gaactggtga tcccgattca ggcctatgca taccggatga atacccgcat gaatccagtt
    11401 gccgaacgct tctggcgtga actgcgcgag ctggagattg tgcttagctg acattctcca
    11461 gcgtcgcact gcgccaaccg ccattatccc agaccagatc aaacgttagc gattcatcgt
    11521 cgtgatacag cattatctca aggctgatcg ccagagtgtg gcagtacgct tctttcaaat
    11581 taacctgcaa cgccgcaatc cgtggctcat gaaataacag tgcagaacgc agctgacggc
    11641 aataccagct ctggagatgt ttgtcgctcg ggttatgcac ttgccactcg ggcatgcctg
    11701 aaacaagtaa tgacggtaat gccgaatgag gagaattaaa aagagtgagg agatcgctgc
    11761 atactgattt acgcagcggt gacgttttat tgcctgaaat ttcaggtaaa acagcctgaa
    11821 gtgattggcg catcattttg cgcactcctg acgtttagca acatatgcgg cttgccctga
    11881 acaccgggca ccactgtatt gatgctcatt ttccatagcc attccctttt gttcattaca
    11941 tccatgtcag tctgagacag gcctgaatcc tgtcagcaaa gtataaaacc ttacttgcct
    12001 atgaatatct actgactttt caggaaaaaa gtcatgattt gataaaaatc atcaaactca
    12061 aaaaaaaacc gctagtatat tctgaatatt ccgaatagtt acgtagatga aaatacttgt
    12121 caccaccgct acttaattcc ggtgggaaat tatttatttc cgcataatca gctatgtatt
    12181 atttaaaaca attagtttgt aaataatata aattgataac atatcaaata catcaaaaat
    12241 accattcaca ggcattatta atatagacat caatatagca acatcaaatt ccaattagta
    12301 ataagatatg cttttaaatt gcatgcaaat aattttaaat gagcgttttt tgatacttta
    12361 gagtgtttcc caccatttaa tatcactgtg tagagttga
//
LOCUS       DAOFQD010000138        12365 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.138, whole genome shotgun sequence.
ACCESSION   DAOFQD010000138 DAOFQD010000000
VERSION     DAOFQD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.138"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            1..2101
                     /locus_tag="QBF46_003955"
                     /pseudo
     CDS             1..2101
                     /locus_tag="QBF46_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2437..3414
                     /gene="csiD"
                     /locus_tag="QBF46_003956"
     CDS             2437..3414
                     /gene="csiD"
                     /locus_tag="QBF46_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDQ6676076.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTFIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNIDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            3434..4702
                     /gene="lhgO"
                     /locus_tag="QBF46_003957"
     CDS             3434..4702
                     /gene="lhgO"
                     /locus_tag="QBF46_003957"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDQ6676077.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRFDNCGKMLVATSPLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQD
                     RGGEIIYNAEVSALNEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4725..6173
                     /gene="gabD"
                     /locus_tag="QBF46_003958"
     CDS             4725..6173
                     /gene="gabD"
                     /locus_tag="QBF46_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDQ6676078.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6187..7467
                     /gene="gabT"
                     /locus_tag="QBF46_003959"
     CDS             6187..7467
                     /gene="gabT"
                     /locus_tag="QBF46_003959"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDQ6676079.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            7705..9105
                     /gene="gabP"
                     /locus_tag="QBF46_003960"
     CDS             7705..9105
                     /gene="gabP"
                     /locus_tag="QBF46_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDQ6676080.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9126..9788
                     /gene="csiR"
                     /locus_tag="QBF46_003961"
     CDS             9126..9788
                     /gene="csiR"
                     /locus_tag="QBF46_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDQ6676081.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9789..10238)
                     /gene="kbp"
                     /locus_tag="QBF46_003962"
     CDS             complement(9789..10238)
                     /gene="kbp"
                     /locus_tag="QBF46_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDQ6676082.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10322..10480)
                     /locus_tag="QBF46_003963"
     CDS             complement(10322..10480)
                     /locus_tag="QBF46_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDQ6676083.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10663..10962
                     /locus_tag="QBF46_003964"
     CDS             10663..10962
                     /locus_tag="QBF46_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6676084.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10972..11496
                     /gene="ygaP"
                     /locus_tag="QBF46_003965"
     CDS             10972..11496
                     /gene="ygaP"
                     /locus_tag="QBF46_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDQ6676085.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11543..11947)
                     /gene="stpA"
                     /locus_tag="QBF46_003966"
     CDS             complement(11543..11947)
                     /gene="stpA"
                     /locus_tag="QBF46_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDQ6676086.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         3126 a         3033 c         3144 g         3062 t
ORIGIN      
        1 accctcaagg attaaaagac agagaaatta tatcaacttt tcacgcagaa aaagttatta
       61 atgatctgtt aaaaaacgat tataaaattt ccctttctcc agatataact taccgagagt
      121 tgaataaagc agcacagcgt agcattacag cgccagacag gataggagaa tgaaaaacat
      181 aggtttatca acgagataca atgattgaaa gaggtgataa cagcggtgtt tatcagtatg
      241 gtcgtgctga acatttcacc cacattatat ctgacaaacc ttccccaaaa gataaatatg
      301 ttgcatatgc tattaacatt cctgactatg agctggcagc cgatgtatat aatattaacg
      361 tgacgtcacc ttccggacag caagaaacat ttaaaatatt aatcaatcca gaacatctac
      421 ggcaaacact tgagcgtaaa tctcttactg ctgttcagaa atcacaatgt gaaatcatca
      481 cccccaaaaa acctggcgaa gcgattcttc atgcttttaa tgccacctac cagcagatca
      541 gagaaaatat gtctgaattt gcacgttgcc attatgggta tatacaaatc cctccagtga
      601 caactttccg tgccgacgga ccagaaactc ccgaagaaga aaaaggttac tggtttcacg
      661 cttatcaacc cgaagatctt tgtaccatcc acaatccaat gggagatttg caggatttta
      721 tcgcattggt taaagatgct aaaaaatttg gtatcgatat cattcctgat tataccttta
      781 actttatggg aatcgggggt agtggtaaaa atgacctgga ttatccctct gctgatatac
      841 gagcgaagat cagtaaagat atagaaagtg gtatccctgg ctattggcaa ggtcaggttt
      901 tgattccatt tactatagat ccagtaacaa aagaacgtaa acaaatccat ccagaagata
      961 tacatctcac tgcaaaagac ttcgaagcaa gtaaagataa tatttctaag gatgaatggg
     1021 aaaacctcca tgcattaaaa gaaatcgttt aaatggaatg cctaaaacaa cccccaaaag
     1081 tgaccaggtt attatgctgc aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg
     1141 aggtttacgt tacgatgcag caaaacactc aaaacatgaa caaatagaaa gatcaataac
     1201 cccaccgctt aaaaattata atgagcgatt acacaatact aacttattta acccaaaata
     1261 tcatgaaaaa gtcgttatga attacatgga atatctggta acttgtcagt tggatgaaga
     1321 acaaatgtca tcgctgcttt atgaaagaga tgatttaagc gccattgatt tttcattgct
     1381 catgaagacg ataaaagctt tttcatttgg tggcgatctc caaacccttg catcaaaacc
     1441 gggttccaca atctcaagca tcccatcaaa aagacggata ttgattaaca ttaaccacga
     1501 ttttcctaac aatggcaatc ttttgaatga ctttctattt aaccatcaac aagatgaaca
     1561 attagcaatg gcatatatgg ccgctctccc gttcagcagg cctttagttt actgggatgg
     1621 ccaagtatta aaatcaacga ctgaaattaa aaattatgat gggtcgacgc gtgttggcga
     1681 tgaggcgtgg cttaataaag gttgctctac ctatcagcag ctctacaatg aattccacgc
     1741 attatatata gataaagcag gaatatggag cgcatttgag ggtgtatttg caactaaaaa
     1801 cgttctggcc tttagtcgtg gggattctgt gaacattaat cactctcctc atgatggact
     1861 aattataata aataaaggaa acgaagaagt tgaaggtgcc tggcctaaca aattgcaacc
     1921 tggaaaatat aaaaacatgg ggagtaatag cgttaacatt attattatta atacccgaaa
     1981 aattatcccc cctggtaaag catttatgct tagaggcgga actctaaata tcaatattcc
     2041 tgggcgtagc gctcttcttt tagggaaaac aggagaaccg ctgaactatc tctatttgta
     2101 atttaatgtt atatctgccc cgctaaaacg gggcagataa tatgtttagt ttactaacgg
     2161 tcattttgca ttgaagccat ttactgtttt ttatcgacca gataatctgt tctctgatgg
     2221 taactccccc taacctattg ctttagttat tcatttcctg tctcactttg ccttaatacc
     2281 ctacgttaaa tgttactaat ttattgcttt tgatcacaat aagaaaacaa tatgtcgctt
     2341 ttgtgcgcat ttttcagaaa tgtagatatt tttagattat ggctacgaaa tgagcatcgc
     2401 catgtcaccc tacatctcat aagaggatcg cttctgatga atgcactgac cgccgtacaa
     2461 aataacgctg tcgattcagg ccaggactat agcggattca ctttcatccc gtcggcgcaa
     2521 tccccgcgtc tgctggaact taccttcacc gaacagacga ccaaacagtt tctcgaacag
     2581 gttgccgaat ggcccgtaca ggcgctggag tacaaatcct ttctgcgttt tcgggtaggc
     2641 aaaattcttg acgatctgtg tgcgaatcag ctgcaaccat tgctgttgaa gaccctgcta
     2701 aaccgtgctg aaggtgcgct gttgatcaat gcggtgggta tcgatgatgt ggcgcaggca
     2761 gatgagatgg tgaagctggc aacggcggta gcgcatctga ttggtcgctc aaatttcgat
     2821 gcgatgagcg gtcagtatta cgcgcgtttc gtggtgaaaa atatcgataa ctcagacagc
     2881 tatctgcgtc agccgcaccg cgtgatggaa ctgcacaacg acggcacgta tgtcgaagag
     2941 atcaccgatt acgtgctgat gatgaaaatc gatgagcaaa acatgcaggg tggaaattcg
     3001 ttgctgctgc atctcgatga ctgggaacat ctggaccact atttccgcca tccactggcg
     3061 cgtcgcccga tgcgctttgc cgcgccgccg agcaaaaacg tcagcaaaga tgttttccat
     3121 ccagtgttcg acgttgatca acagggtcgc ccggtgatgc gctatatcga ccagttcgtc
     3181 cagccaaaag attttgaaga aggcgtgtgg ttgagcgagc tttccgatgc cattgaaacc
     3241 agcaaaggca ttctttctgt acccgttccc gttggcaaat tcctgttgat aaacaacctg
     3301 ttctggctgc acggtcgcga ccgctttact ccgcacccgg atctgcgccg tgaactgatg
     3361 cgtcagcgtg gctatttcgc ttacgcctct aaccactacc agacgcatca gtaagcgcaa
     3421 aggaattgag cggatgtatg attttgtgat tattggcggc ggcatcatcg gcatgtcgac
     3481 cgccatgcaa ctgattgatg tctatccgga tgcccgcatt gcgttgctgg aaaaagagtc
     3541 cggcccggcc tgtcaccaga cgggccacaa cagcggcgtg atccatgccg gggtctatta
     3601 cacgcccggt agcctgaagg cacagttttg cctggcggga aaccgcgcca ctaaagcctt
     3661 ttgcgatcaa aacggcattc gctttgacaa ctgcggcaag atgctggtcg ccacgtctcc
     3721 gctcgaaatg gaacggatgc gcgccttatg ggaacgcaca gcggcgaacg gtatcgagcg
     3781 cgaatggcta aacgccgacg aactgcgcga gcgcgaaccg aatatcaccg ggctcggcgg
     3841 tatttttgtg ccgtccagcg gcattgtcag ctaccgcgaa gtgacagcgg cgatggcaaa
     3901 aatctttcag gacagaggcg gcgagattat ttataacgcc gaagtcagcg cccttaatga
     3961 gcataaaaac ggcgtggtga tacgtacccg tcagggcggt gaatatgaag catcgacgct
     4021 gattagctgt tccgggctga tggctgaccg gctggtgaaa atgctcggac tcgaaccggg
     4081 ctttatcatc tgcccgttcc gtggcgagta tttccgcctt gcgccggagc ataaccagat
     4141 tgttaaccac ctgatttacc ccatccccga cccggcaatg ccgtttttgg gcgtgcatct
     4201 cacccgcatg atcgacggca gcgtgaccgt cgggccaaac gcggtgctgg ctttcaaacg
     4261 tgaaggctat cgcaagcgcg acttctcatt tagcgacacg ctggaaattt tgggctcgtc
     4321 ggggattcgc cgggtgctgc aaaaccatct acgctcagga ctgggcgaga tgaaaaactc
     4381 gctgtgcaaa agcggctatc tgcggctggt gcaaaagtat tgtccccggc tttcgttaag
     4441 cgatctccag ccctggcccg ccggtgtgcg ggcgcaggcg gtatcgccgg acggcaagct
     4501 gattgacgat tttctgtttg tcaccacccc gcgcacgatc cacacctgca atgcgccctc
     4561 cccggcagcg acatcagcaa ttcctattgg tgcgcatatt gtcagcaagg tacaaacgct
     4621 gttggcaagc cagagtaacc ccggacgcac gctgcgagcg gcacgtagtg tggatgcctt
     4681 acacgccgca tttaatcaat aacctttgaa aacaggacgt aacaatgaaa cttaacgaca
     4741 gtaacttatt ccgccagcag gcgttgatta acggggaatg gctggacgcc aacaatggcg
     4801 aggtcatcga cgtcaccaat ccggcgaacg gcgacaagct gggtagcgtg ccgaaaatgg
     4861 gcgcggatga aacccgcgcc gctatcgacg ccgccaaccg cgccctgcca gcctggcgcg
     4921 cgctcaccgc caaagaacgc gccaccattc tgcgcaactg gttcaatttg atgatggagc
     4981 atcaggacga tttagcgcgc ctgatgaccc tcgaacaggg taaaccgctg gctgaagcga
     5041 aaggcgaaat cagctacgcc gcttccttta ttgagtggtt tgccgaagaa ggcaaacgca
     5101 tttatggcga caccattccc ggtcatcagg ccgataagcg cctgattgtt atcaagcagc
     5161 cgattggcgt taccgccgcc atcacgccgt ggaacttccc ggcggcgatg attacccgta
     5221 aagccggtcc ggcgctggcg gcaggctgca cgatggtgct gaaacccgcc agtcagacgc
     5281 cgttctctgc gctggcgctg gcggagctgg cgattcgcgc gggcattccg gctggcgtat
     5341 ttaacgtggt caccggttcg gcgggcgcgg tgggtaacga actgaccagc aacccgctgg
     5401 tgcgcaaact gtcgtttacc ggttcgaccg aaattggccg ccagttaatg gaacagtgcg
     5461 cgaaagacat caagaaagtg tcgctggagc tgggcggcaa cgcgccgttt atcgtctttg
     5521 atgatgccga tctcgacaaa gccgtggaag gcgcactggc ctcgaaattc cgcaacgccg
     5581 ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga cggcgtgtat gaccgctttg
     5641 ccgaaaaatt acagcaggcg gtgagcaaac tgcacatcgg cgacgggctg gataaaggcg
     5701 tcaccatcgg gccgctgatc gatgaaaaag cggtagcaaa agtggaagag catattgccg
     5761 atgcgctgga gaaaggtgca cgtgtggttt gcggcggtaa agcggatgaa cgcggcggca
     5821 acttcttcca gccgaccatt ctggtggacg ttccagccaa cgccaaagtg tcgaaagaag
     5881 agacgttcgg ccccctcgcc ccactgttcc gttttaaaga tgaagccgat gtgattgcgc
     5941 aagccaatga caccgagttt ggccttgccg cctatttcta cgcccgtgat ttaagccgcg
     6001 tcttccgcgt gggcgaagcg ctggagtacg gcatcgtcgg catcaatacc ggcatcattt
     6061 ccaatgaagt ggccccgttc ggcggcatta aagcctcggg tctgggtcgt gaaggttcga
     6121 agtatggcat cgaagattac ttagaaatca aatatatgtg catcggtctt taactggaga
     6181 acgcgaatga gcagcaataa agagttaatg cagcgccgca gtcaggcgat tccccgtggt
     6241 gttgggcaaa ttcacccgat tttcgctgac cgcgcggaaa actgccgggt gtgggacgtt
     6301 gaaggccgtg agtatcttga tttcgcgggc gggattgcgg tgctcaatac cgggcacttg
     6361 catccgaaag tggtggctgc ggtggaagcg cagttgaaaa aactgtcgca cacctgcttc
     6421 caggtgctgg cctacgagcc gtatctggag ctgtgcgaga ttatgaatca gaaggtgcca
     6481 ggcaattttg ccaagaaaac gctgctggtc accacaggtt ctgaagcggt ggaaaacgcg
     6541 gtgaaaatcg cccgcgccgc caccaaacgt agcggcacca tcgcttttag cggcgcgtat
     6601 cacgggcgca cgcattacac gctggcgctg accggcaagg tgaatccgta ctctgcgggc
     6661 atggggctga tgccgggtca tgtttatcgc gcgctttatc cttgcccgct gcacggcata
     6721 agcgaggatg acgctatcgc cagcatccac cggatcttca aaaatgatgc cgcgccggaa
     6781 gatatcgccg ccatcgtgat tgagccggtt cagggcgaag gcggtttcta cgcctcgtcg
     6841 ccagccttta tgcagcgttt acgcgctctg tgtgacgagc acgggatcat gctgattgcc
     6901 gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt ttgcgatgga gcagatgggc
     6961 gttgcgccgg atcttaccac ctttgcgaaa tcgatcgcgg gcggcttccc gctggcgggc
     7021 gtcaccgggc gcgcggaagt aatggatgcc gtcgctccag gcggtctggg cggcacctat
     7081 gcgggtaacc cgattgcctg cgtggctgcg ctggaagtgt tgaaggtgtt tgagcaggaa
     7141 aatctgctgc aaaaagccaa cgatctgggg cagaagttga aagacggatt gctggcgata
     7201 gccgaaaaac acccggagat cggcgacgta cgcgggctgg gggcgatgat cgccattgag
     7261 ctgtttgaag acggcgatca caacaagccg gacgccaaac tcaccgccga gatcgtggct
     7321 cgcgcccgcg ataaaggcct gattcttctc tcctgcggcc cgtattacaa cgtgctgcgc
     7381 atccttgtac cgctcaccat tgaagacgct cagatccgtc agggtctgga gatcatcagc
     7441 cagtgttttg atgaggcgaa gcagtagcgc cgctcctatg ccggaggcga cgctgcgcgt
     7501 cttgtccggc ctacggggat ccaggtcgga taaggcgttt acgccgcatc cgacaatctg
     7561 tacgtgaaca ggaagaaatc tatgttggcc gggtaaggcg gagccgctct ccggcaaaaa
     7621 gaatcaataa caattatacg cgtgacccgg cgcgggaaat gtcggggcgc tctcccaagt
     7681 gacacacttt cgagaggatt caggatgggg caatcatcgc aaccacatga gttaggcggc
     7741 gggctgaagt cacgccacgt caccatgttg tctattgccg gtgttatcgg cgcaagtctg
     7801 tttgtcggtt ccagcgtcgc catcgccgaa gcgggcccgg cggtattact ggcctatctg
     7861 ttcgccgggc tactggtggt tatgattatg cggatgttgg cggaaatggc ggttgccacg
     7921 cccgataccg gttcgttttc cacctatgcc gataaagcca ttggtcgctg ggcgggctat
     7981 accatcggct ggttgtactg gtggttttgg gtactggtta tcccgctgga agccaacatc
     8041 gccgccatga tcctgcactc atgggttcca ggcattccca tctggttatt ttccctcgtc
     8101 attaccctcg ccttaactgg cagtaactta ttaagcgtta aaaactacgg cgaatttgag
     8161 ttctggctgg cgctgtgcaa agtcatcgct atcctggcct ttattttcct tggtgcagtc
     8221 gcaattagcg gtttttaccc gtatgccgaa gtgagcggga tctcaagatt gtgggatagc
     8281 ggcggcttta tgcccaacgg tttcggtgcg gtattaagcg cgatgttgat caccatgttc
     8341 tcgtttatgg gcgcagaaat tgtcaccatt gccgccgcgg aatccgacac gccggaaaaa
     8401 catattgtcc gcgccaccaa ctcggttatc tggcgtattt ctatcttcta tttatgttct
     8461 atttttgtcg tcgtggcatt aattccgtgg aatatgcccg gactaaaagc cgtcggttct
     8521 tatcgctcgg tactggaatt gctcaatatt ccccatgcga aattaatcat ggactgcgtg
     8581 atattacttt ccgtaaccag ttgcctgaac tcggcgctgt ataccgcgtc aaggatgctc
     8641 tactccttaa gccgtcgcgg tgatgctccc gcggtaatgg gtaaaatcaa ccgcagtaaa
     8701 accccgtacg tggcggtgtt actctccacc ggcgcggcat tcttaacggt ggtggtgaac
     8761 tattacgcgc ctgcgaaggt atttaaattt ctgatcgaca gctccggcgc tatcgccctg
     8821 ctggtttatt tagtcatcgc cgtttcacag ttgcggatgc gcaaaattct gcgagcagaa
     8881 ggaagcgaaa ttcgcttgcg aatgtggctt tatccgtggc tcacctggct agtcatcggc
     8941 tttattacct ttgtgttggt agtgatgcta ttccgtccgg cgcaacagtt agaagtgatc
     9001 tccaccggct tattagcgat agggattatc tgtaccgtgc caattatggc tcgctggaaa
     9061 aagctggtat tgtggcaaaa aacacccgtt cataatacgc gctgatcatg atcaggagtc
     9121 acaccatgac cattacgtct ctggatggct atcgctggct gaagaacgat attattcgcg
     9181 gtaattttca accggatgaa aaattacgaa tgagtttgct gacatcgcgt tatgcacttg
     9241 gcgttgggcc gttacgggaa gctctttcgc aactggtggc ggaacggctg gtcacggtgg
     9301 tgaatcaaaa agggtatcgg gtggcgtcta tgtcagagca ggagctgctc gatattttcg
     9361 acgcccgcgc caatatggaa gcgatgttag tgagtctggc gattgcccgc ggtggcgatg
     9421 agtgggaggc agacgttctc gcaaaagcgc atctgctgag taagcttgag gcctgtgacg
     9481 ccagcgagaa aatgcttgat gagtgggatc tgcgtcatca ggcgtttcat acggcaattg
     9541 tggcgggctg tggttctcac tatttgctgc aaatgcgtga acggttgttt gatctggcgg
     9601 cgcgttatcg atttatctgg ctgcggcgaa cggtgctttc ggtggaaatg ctggaggata
     9661 aacacgatca gcaccagacc ctgactgcgg cggtactggc gcgagatacc gcgcgcgcca
     9721 gtgagttaat gcgccagcat ttactgacgc caattcccat tatccagcag gcgatggctg
     9781 gcaattaact actcttccgg aatacgcaac acttgccccg gataaatttt atccgggctt
     9841 tttagcatcg gtttattcgc ttcgaagatt ttattgtaca gattagcgtt accgtagacc
     9901 tgtttggaaa tggcactcag agtgtcgcca gacttaacgg tataaaactg gctggcagtg
     9961 gctggtgtcg ccgttttcac ctgatcatcg acactggcaa taccggaaat attccccacc
    10021 gcaacaagga ttttctcctt cgcctcctga ctcaggccgt caccagtgac cgtcgctttg
    10081 ccgtcggcaa tttgaatatt cactttatcg gcatccggta taccggtttt gttcagatgc
    10141 tcctgcacct tcttcgcctg atcgtcttta tcgtgctgac ctgtaaccgc gtcccagagt
    10201 ttttctccgg catctttcac aaaattgaac agacccatag ctacctcatt cgttaacgga
    10261 aacactcaga aagtgtagca gaggaacgcg gcgcgttaac gccgcgggga tgagcacagg
    10321 tttaatcgcg ggtttgcacc cagaacgcgt gaatcaaacc ggggatatac ccaagcagtg
    10381 tcaacagaat attaataatg aacgcccaac cgaacccttt accgagcagc acgccgagcg
    10441 gcggcagaat gatggtgatg acgattctcc agaaacccat atgtactccc tataagaaaa
    10501 ttactcattg tttaaaaaga gattttatct cttaagcgta gtaattttag cggagcctgc
    10561 cagtttcagc ttacgctttt actctcattt acgctgctga ggctggtata tgttgcattt
    10621 ttagtttaga gtttacttaa tttagaaaat acttaaataa atatgactga actcgcgcaa
    10681 ttacaggcca gtgccgaaca ggcagcggcc ttattgaaag caatgagcca ccctaaacga
    10741 ttgctgattc tgtgcatgct tagcggttcc cccggcacca gcgcgggaga gctgacgcgc
    10801 attaccggac tgagtgcctc tgcgacatca cagcatctcg ctcgtatgcg ggacgaaggg
    10861 cttatcgaca gccaacggga tgcccaacgc attctatatt ccattaaaaa tgaggcggta
    10921 aatgccatta tcgccaccct gaaaaatgtc tattgtccgt aaggagtcac tatggctttg
    10981 acaaccattt cgccgcatga tgcacaagaa ttaatcgcac gcggcgcaaa gttaatcgat
    11041 attcgtgatg ctgatgaata tcttcgtgaa catattcctg aagcagatct ggctccatta
    11101 tccgtgctgg aacagtcagg tcttccggct aaattacgtc acgagcaaat tatattccac
    11161 tgccaggcag gtaaacgcac cagtaataat gccgataaat tagcagcgat tgccgccccc
    11221 gcagaaatct ttttactcga agatgggatt gatggctgga aaaaagcagg attgccagta
    11281 gcggtaaata aatctcaacc cttgccgtta atgcgccagg tgcagatcgc tgcgggtggt
    11341 ttaatattaa tcggcgttgt actgggttat accgtaaata gcggtttctt cttattaagt
    11401 ggctttgttg gtgccgggtt actgtttgca ggaatcagcg gtttttgtgg aatggcaagg
    11461 ttgttagata agatgccgtg gaaccaacga gcttgagaag cgacgccgga cgcgccctag
    11521 cagcgacatc cggcctcagt aattagatca ggaaatcgtc gagagattta ccttctgcca
    11581 gcgcctgagc aattggcttc ggtgtacggc cctgaccggt ccaggtttta gtttcaccgt
    11641 taacatcggt gaatttatat ttcgccggac gcggctggcg ttttttacca gcgcgtggtg
    11701 cagcagcaga gctattaccc aataactctt ccgggttaat tccgtcagct ttcatcagct
    11761 ccagccaggt gctaattttt tcctggcgct ctgccagttc acgctgctgc tgttcttctt
    11821 cttcacgtct ttctttagtg acaaccctga atttttcgag catttcttca agaacgtcaa
    11881 tggagaattc gcgagccatc gcacggaggg tgcgaatgtt atttaaactt tgtaacatta
    11941 cggacataat aaagaaaacc ttttaacgcc aaaacaaaaa aagtatttcg tcgcgtagtg
    12001 taagcgatca cttattatat ttccacaaaa tttaatctat ttacagtcct gcgcgagatt
    12061 atttcagctg ttattttctg aaactctcag aataatttca agattatata ttagccattt
    12121 tgatgagtaa tttagattat tggtgaacag agcggcgctc tctgctcgct aattccgcaa
    12181 gcctgacaaa accaaagtga ataaaaatgc aatataacag aataaaagaa aacatccatc
    12241 actggtgctt tctgctgtta atgtacattg tctggcataa aaaatgctga aaatgaccca
    12301 gctactacat acaccgaatt ctctacgctt atagcgttac ctcaccccca aactttcatt
    12361 agtga
//
LOCUS       DAOFQD010000139        12299 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.139, whole genome shotgun sequence.
ACCESSION   DAOFQD010000139 DAOFQD010000000
VERSION     DAOFQD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.139"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..882)
                     /locus_tag="QBF46_003967"
     CDS             complement(<1..882)
                     /locus_tag="QBF46_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6676087.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSA"
     gene            complement(928..1752)
                     /locus_tag="QBF46_003968"
     CDS             complement(928..1752)
                     /locus_tag="QBF46_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6676088.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(1835..2089)
                     /gene="yoeB"
                     /locus_tag="QBF46_003969"
     CDS             complement(1835..2089)
                     /gene="yoeB"
                     /locus_tag="QBF46_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDQ6676089.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(2086..2337)
                     /gene="yefM"
                     /locus_tag="QBF46_003970"
     CDS             complement(2086..2337)
                     /gene="yefM"
                     /locus_tag="QBF46_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDQ6676090.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            2620..2670
                     /gene="hisL"
                     /locus_tag="QBF46_003971"
     CDS             2620..2670
                     /gene="hisL"
                     /locus_tag="QBF46_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDQ6676091.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    2646..2769
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            2816..3715
                     /gene="hisG"
                     /locus_tag="QBF46_003972"
     CDS             2816..3715
                     /gene="hisG"
                     /locus_tag="QBF46_003972"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDQ6676092.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            3721..5025
                     /gene="hisD"
                     /locus_tag="QBF46_003973"
     CDS             3721..5025
                     /gene="hisD"
                     /locus_tag="QBF46_003973"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDQ6676093.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDMVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            5022..6092
                     /gene="hisC"
                     /locus_tag="QBF46_003974"
     CDS             5022..6092
                     /gene="hisC"
                     /locus_tag="QBF46_003974"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDQ6676094.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQLSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            6092..7159
                     /gene="hisB"
                     /locus_tag="QBF46_003975"
     CDS             6092..7159
                     /gene="hisB"
                     /locus_tag="QBF46_003975"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDQ6676095.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            7159..7749
                     /gene="hisH"
                     /locus_tag="QBF46_003976"
     CDS             7159..7749
                     /gene="hisH"
                     /locus_tag="QBF46_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDQ6676096.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            7749..8486
                     /gene="hisA"
                     /locus_tag="QBF46_003977"
     CDS             7749..8486
                     /gene="hisA"
                     /locus_tag="QBF46_003977"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDQ6676097.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            8468..9244
                     /gene="hisF"
                     /locus_tag="QBF46_003978"
     CDS             8468..9244
                     /gene="hisF"
                     /locus_tag="QBF46_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDQ6676098.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            9238..9849
                     /locus_tag="QBF46_003979"
     CDS             9238..9849
                     /locus_tag="QBF46_003979"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDQ6676099.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPQALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(9946..10926)
                     /gene="wzzB"
                     /locus_tag="QBF46_003980"
     CDS             complement(9946..10926)
                     /gene="wzzB"
                     /locus_tag="QBF46_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDQ6676100.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVPDLQEALIGRFSSA
                     FSALAETLDNQKEPEKLTIEPSVKNQQLPLTVSYVGQTAEDAQMKLAQYIQQVDDKVN
                     QELEKDLKDNLALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNSK"
     gene            complement(11073..12239)
                     /gene="ugd"
                     /locus_tag="QBF46_003981"
     CDS             complement(11073..12239)
                     /gene="ugd"
                     /locus_tag="QBF46_003981"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDQ6676101.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQADKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         3044 a         3189 c         3140 g         2926 t
ORIGIN      
        1 cgcactattt gctaaaattt catcccgcca ccagccgctg gcaagccctg cggtctgttc
       61 attccagcca atccagacgg gttcaaagag agtttgggcg accactttct tctcgattaa
      121 cgcgccacta tcgagaaacc gttgcgccag ataacggggt aaatagccgc agccgaggcc
      181 gctaatttgc agctccagct tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc
      241 gagaagttgt gaggccgtag aagcgcctgc ctgcgcagta tcccccacca caatcgcccg
      301 atatcgctta ataatacgac ggtttaacgg ctcttcttcc aaggctaacg gatgatgagg
      361 cgcgaccgca aagacttgtt ccagatcgcc caaccgtgaa aaaccaaact cactggaaga
      421 tggtggctca tgcatcgcgc caacaataat atccgctcgc ccctgagtca gggcatccca
      481 ggaaccaccg agtacgccat tgataaactt caggcgcgtc acgctatgat gttgataaaa
      541 agcttcaatc agtggcgcaa gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag
      601 ctcgttttcc cagccttcat gcagtttgat cgcctgcttt tccagttctc gcacggtatg
      661 cagaacttcc cgccctttct ctaataacat ttttccggta cgggtgaatt tagcgcggtg
      721 accgctacga tcaagcaatt gaatattaag gtcgctttcc agcttgtgaa cggtgtaact
      781 cagagcggac ggcgtcttat agagtttggc cgacgccgcc gcaaaactgc cttctttttc
      841 taaggcatcg aggatcatca acacatccag cagtggtttc atacacgccc ccttgcagtg
      901 catgcggcgt cctgctggcg tgatcgttta ctccagcggc attaccagcg gatcgggata
      961 ctggtattca aatcccagtt cattacaaat ccgactgcca tcaataatct tacctttgcc
     1021 gctgtccaga ctatttctga actgcggagg ctccagcccc agtaaacggg ccatctgcgg
     1081 atagaaaaca ttacgcgcag ggtgagctgg cgcacatata ttatagatgt gtccgccttt
     1141 aggtgcctgt aacaacagag tgatagcgcc aataacatct tctaaatgga ctaaattaac
     1201 accatgttca ccatcaggcg cggtttttcc ggcaaagaag cgtccgggat gacgtcccgg
     1261 tccgaccagc cccgcaagac gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca
     1321 gtcttcgagt tcttctaata ctcgtccact gttggttacc ggattacgcg gggtagtttc
     1381 tttcaccgtg ccttgcgcgt cgccatagac agatgttgag ctggtaaaaa taattcgggg
     1441 aatacgatgg gccagcgcgc tatccactaa ctcttgtacc gcttgtaaat agaactcatc
     1501 gccggggccg ctacgacgtg ccggaagcgt aatgaccagc gcgtcggcat ccatcagggc
     1561 atccagatcg tcagaatcgc aaactaactc aggctccatg cgaagcagat agctatcaat
     1621 gccactcatt cgggccgctt cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca
     1681 gcctcgcgct gaaagtgaca tcgccagcgg catgcctaac caccctaacc cgacaattgc
     1741 gacctttttc atccgttatc tcctgataca ccttagatct ataaggctac gctagcgtat
     1801 caaaactgac aattcattct atgaatgaat ctgttcaata atgataacga catgctgcaa
     1861 tgagcagtga atcgtcggta accgcgtata ccagacggtg ctcctctgta atgcgtcggg
     1921 accagaaacc tgacaaatta tgtttcaggg gttctggctt ccccttacct tcaaatggcg
     1981 ttctgcgggt atctttgata agttcattga tctttttaac aattcgctta tctgtttcct
     2041 gccagtacag ataatcatcc catgattcct cagaccagat tagtttcact caataatgtc
     2101 cttttctgtt cctttgcctg atttcaggct atcgattgag tccatcaatc tccgggcgtt
     2161 agcgggggag cgcagtagat aagccgtctc ttccagcgag ttgtattctt cgagtgacat
     2221 cagaacacaa gcctctccat tctgacgagt aataaggatc ggggcatgat cttcaacggc
     2281 tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg tacgcatgtc
     2341 aatctcctct tttgtacagt tcattgtaca atgatgggcg ttaattaact atttattaat
     2401 tagtttgtag atcaaggtat tgtcagtgag acgaaaatcc aggcttcgct atttttggtg
     2461 ccgtcagctt agaggacagt cctcttagcc ccctcctttc cccgctcatt cattaaacaa
     2521 atccattgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag tggtttaggt
     2581 taaaagacat cagttgaata aacattcaca gagactttta tgacacgcgt tcaatttaaa
     2641 caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
     2701 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
     2761 cttttttatt gcgcgattga taacggttca gacaggttta aagaggaata agaaaatgac
     2821 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
     2881 tgaattgctg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
     2941 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gacgacattc ccggtctggt
     3001 aatggacggc gtggtagatc ttgggattat cggcgaaaac gtgctggaag aagagctgct
     3061 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     3121 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggcc cgctctcctt
     3181 aaatggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     3241 aggcatctct tttaaatcct gcttactgaa cggttctgtc gaagttgccc cgcgcgccgg
     3301 actggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag caaacggcct
     3361 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atacaacgcg atggcgaaat
     3421 ggaagaatcc aaacagcaac tgatcgacaa actgctgacc cgtattcagg gtgtgattca
     3481 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgtctgg atgaagtcat
     3541 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg accaacagcg
     3601 cgtagcgatg cacatggtca gcagtgaaac cctgttctgg gaaacgatgg aaaaactgaa
     3661 agcgctgggt gccagttcaa ttctggtcct gccgattgag aagatgatgg agtgatcgcc
     3721 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     3781 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcactgttaa cgatattctc
     3841 gataacgtga aagcacgcgg tgatgacgcc cttcgggaat acagcgcgaa gtttgataaa
     3901 accacggtta acgcgctgaa ggtgtctgct gaggagattg ccgccgctag cgaacgcctg
     3961 agcgacgagc taaaacaggc gatggcggtg gcagtaaaaa atattgaaac cttccacact
     4021 gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg ccagcaggtc
     4081 acgcgcccgg tcgcttcagt tgggttgtat attcctggcg gctccgcccc gctcttctca
     4141 acggtattaa tgctggcgac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     4201 tcaccgccgc cgattgccga tgagatcctt tatgcggcgc agctgtgcgg tgtgcaggac
     4261 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     4321 ccgaaagtgg acaaaatctt cgggccagga aacgcctttg tcaccgaggc aaaacgtcag
     4381 gtgagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgttg
     4441 gtcattgccg acagcggcgc aacgccggat ttcgtggctt ctgatttgct ttctcaggct
     4501 gaacacggcc cggactcaca ggtgatttta ctgacgcccg ctgctgatat ggcgcgtcgc
     4561 gttgccgaag ctgtcgaacg ccaactggca gaactgccgc gtgccgaaac cgcccgtcag
     4621 gcactgagcg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt agagatctcc
     4681 aaccagtacg gcccggagca cctgatcatc cagacccgca acgcccgcga tatggtcgat
     4741 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggtgat
     4801 tacgcctccg gcaccaacca cgttctgccg acttacggtt acaccgccac ctgttccagc
     4861 ctcgggctgg cggatttcca gaagcggatg accgtgcagg aactgtcgaa agtaggtttc
     4921 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     4981 aatgccgtta ctttgcgtgt taacgccctt aaggagcaag catgagcacc gtgactatta
     5041 ccgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg cgtcgtctgg
     5101 gcggtaatgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttcagc
     5161 ttactcagca aacgctcaac cgctacccgg aatgccagcc gaaagcggtg attgaaaatt
     5221 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     5281 gtattgaact actgattcgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     5341 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggcgtcgag tgccgcacag
     5401 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     5461 taaaagtggt ctatgtttgc agccccaaca acccaaccgg gcaactgatc aatccgcagg
     5521 attttcgtac cctgctggag ttaacgcgcg gtaaggcgat tgtggttgcc gatgaagcct
     5581 atatcgagtt ttgcccacag gcatcgctgg ctggctggct ggcggaatat ccgcacctgg
     5641 ctattttgcg cacactgtcg aaagcttttg ctctggcggg ccttcgttgc gggtttacgc
     5701 tggcaaacga agaagtcatc aacctgctga tgaaagtgat cgccccctac ccgctctcga
     5761 cgccggttgc cgacattgcg gcccaggcgt taagcccgca ggggatcgtc gccatgcgcg
     5821 aacgggtggc gcaaattatt gcagaacgcg aatacctgat tgccgcactg aaagagatcc
     5881 cctgcgtgga gcaggttttt gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     5941 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     6001 aactctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     6061 tcattgacgc cttacgtgcg gagcaagttt aatgagtcag aagtatcttt ttatcgatcg
     6121 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     6181 cgcctttgaa ccgggcgtga tcccggaact gctgaagctg caaaaagcgg gctacaaact
     6241 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcagg cggattttga
     6301 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     6361 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     6421 ggtagagcgt tatctggctg agcaagcgat ggatcgcgcc aacagttatg tgattggcga
     6481 tcgcgcgacc gatattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     6541 cgaaaccctg aactggccga tgattggcga gcaactcact aaacgagacc gttacgccca
     6601 tgtagtgcgc aacaccaaag agacgcagat tgacgttcag gtgtggctgg atcgcgaagg
     6661 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     6721 ccacggcggt ttccgtatgg aaatcaacgt caaaggcgac ctctatatcg acgatcacca
     6781 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgataa
     6841 acgcggtatt tgccgctttg gttttgtgct accgatggac gaatgccttg cccgctgcgc
     6901 gctggatatc tctggtcgcc cacacctgga atataaagcc gagtttacct accagcgcgt
     6961 gggcgatctc agcaccgaaa tgatcgagca cttcttccgt tcgctctcat acaccatggg
     7021 cgtgaccctg cacctgaaaa ccaaaggtaa aaacgatcac caccgtgtag agagcctgtt
     7081 caaagccttt ggtcggaccc tgcgccaggc catccgcgtg gaaggcgata ccctgccctc
     7141 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caatctgaac
     7201 tcggtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     7261 gtcgtgttgc tggccgataa actgttttta cccggcgttg gcaccgcaca agcagcgatg
     7321 gatcaggtcc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     7381 ggcatctgct tagggatgca actgctgggg cggcgtagcg aagagagcaa cggcgtcgat
     7441 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccgctgccg
     7501 catatgggct ggaaccgcgt ttacccgcag gcaggcaacc gcctgtttca ggggattgaa
     7561 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cggttaatcc gtggaccatc
     7621 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     7681 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agctgctgaa aaacttcctg
     7741 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     7801 agggcgatta cggcaaacaa cgtgattacg gtaacgaccc gctgccgcgc ttacaggatt
     7861 acgccgcaca aggtgccgaa gtgttgcatc tggtggatct gaccggagca aaagatccgg
     7921 ctaaacgtca aatcccgctg attaaaaccc tggtggcggg agttaacgtt ccggtgcagg
     7981 ttggcggcgg cgtgcgtagc gaagaagacg tggcggcgtt actggaagcg ggcgttgcgc
     8041 gcgtggtggt cggctccacc gcggtgaaat caccagaaat ggtgaaaggc tggtttgaac
     8101 gttttggtgc cgatgcctta gtgctggcgc tggatgtccg tattgacgag caaggcaaca
     8161 agcaggtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     8221 aaacctattt gcccgtcggc ctgaaacatg tgctgtgtac cgatatctcg cgcgacggca
     8281 cgctggcagg ctctaacgtc tctttatatg aagaagtgtg cgccagatac ccgcaggtgg
     8341 catttcagtc atccggcggc attggcgaca ttaacgatgt tgctgccctg cgtggcactg
     8401 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     8461 ccatagcatg ctggcaaaac gcataattcc gtgtctcgac gttcgtgatg gtcaggtggt
     8521 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccgc tggcaaaacg
     8581 ctacgctgaa gaaggtgcag acgaactggt gttctacgat atcaccgctt ccagcgatgg
     8641 ccgtgtggta gataaaagct gggtatctcg cgtagcagaa gtgatcgaca tcccgttctg
     8701 cgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     8761 taaaatctcc attaactccc ctgcactggc ggacccgacg ttaattactc gcctggcgga
     8821 tcgctttggc gtgcagtgta ttgtggtcgg tattgatacc tggtacgacg gcgaaaccgg
     8881 taaatatcat gtgaatcaat ataccggcga tgaaagccgc acccgcgtca ctcagtggga
     8941 aacactcgac tgggtagagg aagtgcaaaa acgcggtgcc ggagaaatcg tcctcaacat
     9001 gatgaatcag gacggtgtgc gtaacggtta cgacctcgag cagctgaaaa aagtgcgtga
     9061 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctcga
     9121 agccttccgc gatgccgacg ttgacggcgc gctggcagct tccgtattcc acaaacaaat
     9181 aatcaatatt ggtgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     9241 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggact tatgccggtg
     9301 attgtgcaac acgcggtatc cggtgaagtg ttaatgctgg gctatatgaa cccgcaagcc
     9361 ctggacaaaa ccatcgaaag cggcaaagtc accttcttct cgcgcactaa acagcgactg
     9421 tggaccaaag gcgaaacgtc gggcaatttc ctcaacgtag taaatattac gccggactgc
     9481 gacaacgaca cgttactgtt gctggcgaat cccatcggcc cgacctgcca caaaggcacc
     9541 agcagctgct tcggcgacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     9601 ctcgccgaac gtaaatctgc cgacccggaa acctcctata ccgccaaact gtatgccagc
     9661 ggcaccaagc gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagcg
     9721 acagtacatg accgctttga gctgaccaac gaggcatctg atttgatgta tcacctgctg
     9781 gtgctgttgc aggatcagga tctggactta acgacggtga ttgagaacct gcgtaaacgg
     9841 catcagtgag ttgcggggta agcggatgcg atattgtcgt cgcatccggc aaaaaaaacg
     9901 ggcaaggtgt caccaccctg cccattttct ttaaaaccga aaagattact tcgagttgta
     9961 attacgcaga gcattacgcc ccaacacaat ccccgcgcca accatgccgc ccagcagcac
    10021 cgccagaatc aaggtaattg cctttttcgg gctatcgcga cgaataggta acgtcggttt
    10081 catcacataa cggtaagcat gaatatcaag atcgtcaacc tttaagtttt cgatatccag
    10141 caggttttga cgagtctgat agtagtttgg cgaaaacacc aacggacgag tcgcctcatg
    10201 cttaatcatc gactccagcg cttcgctccc caaaaggaac aacgtatctt gtgtgatatc
    10261 ttcgccagtc tgttgaatct gcggttttgt cacctgcgcc tgattcgcat actgcaacgc
    10321 ttcctgaatc tgacggatac gcagatcttt ctgctcctgc gcaaccactt cctgagttct
    10381 taaagagtcc tgcaagtttt tccgtcccaa agcaaggttg tccttgagat ccttttctag
    10441 ctcttgattt actttatcat caacttgctg aatgtattgg gccaatttca tttgtgcgtc
    10501 ctctgcagtt tgcccaacat aagaaacagt caatggtaat tgctggttct ttacagaagg
    10561 ttcgatggta agtttttctg gttctttctg attatccagc gtttctgcta atgcagagaa
    10621 ggcagaactg aagcgaccaa ttaacgcctc ctgtaaatcc ggcactttag gtgcagcctg
    10681 accatagata acattcatgg cattgttata gccagcaatt tgccccacgt caggctgagt
    10741 gataattgct gttgacgtcc atttctcctt cgctaccgcc aaatacccaa tagccagggc
    10801 aatagcaaca atgacagaga tgataattgt catcttgcca cgccacaact gcaccagtaa
    10861 atcaatcaaa tcaatctgtt ctgggtcatg gttttgccca gaaacattat tattttctac
    10921 tctcatcatt accctaacgg acgaaataat tttaagtcgc catagtttag cgataatcgc
    10981 catgaatgct ataggataaa tgataaaaat aatgaattat tacaaaggga acataagaga
    11041 atcagggcga aaatcgccct gataacagga tgttagtcac taccaaagag atcgcgggtg
    11101 tagactttgt ctgccacatc cttaagctct tctgccatac ggttggaaat aatgacatcg
    11161 gcttgttgtt tgaaggtggc gagatcacgt tccagccgag agttgaagaa tgaatcttct
    11221 ttcatcaccg gttcgtagat gatcacttca acgcctttcg ccttgatacg tttcataatc
    11281 ccctgaatag aagacgcacg gaagttatct gaaccgctct tcataatcag acgataaata
    11341 cctaccactt gcggcttgcg tgacaaaatg gcatcggcaa taaaatcttt acgcgtgcgg
    11401 ttagcatcga caattgccga gatcaggtta ttcggcacag actggtagtt cgccagtaac
    11461 tgcttggtat ctttcggcaa gcaataacca ccataaccaa acgacggatt gttgtaatgg
    11521 ttgccaatac gtgggtcgag acaaacgcct tcgattattt gacgggaatt cagacctaaa
    11581 ctttctgcgt agctatccag ttcgttaaag tacgccacgc gcatcgccag gtaggtgttt
    11641 gcaaaaagtt taatcgcttc tgcttcagtg gagtcggtaa acagggtcgg gatattttgc
    11701 ttaatcgcgc cttcctgtaa cagcgcagca aaacgttctg cgcgttctga acgctcaccg
    11761 atgacaatac gtgaaggatg gagattatcg taaagggctt taccctcacg gagaaattcc
    11821 ggagagaaaa taatattttc agtacgatat ttcttatgca tcgctgcggt aaaaccaacg
    11881 ggaaccgttg atttgatgac cataaccgca taaggattta tctcaactac gtctttaatt
    11941 actgattcta cactggatgt attgaaataa ttagttttag gatcatagtc ggttggagtg
    12001 gcgatgatga cataatcagc atcccggtag gcttcatttt tatctaatgt ggcattaaag
    12061 tgtattttat ctgcttgcaa aaactgctga atttccttat caacaatagg agatatccga
    12121 tcattcagca tagcaacgcg tgacggtaaa atatctaatg ccacaacctc atgattttgt
    12181 gcgattagaa gcccgtttga caagcctaca tagccagtac cggaaatggt gattttcatt
    12241 tcatgctctc agaattaact taactgtgaa tcatgatgtt tttagcatcc tgataagag
//
LOCUS       DAOFQD010000140        12193 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.140, whole genome shotgun sequence.
ACCESSION   DAOFQD010000140 DAOFQD010000000
VERSION     DAOFQD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.140"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..330
                     /locus_tag="QBF46_003982"
     CDS             <1..330
                     /locus_tag="QBF46_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDQ6676102.1"
                     /translation="PGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQ
                     QQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALD
                     REGVNNG"
     gene            323..1387
                     /gene="proW"
                     /locus_tag="QBF46_003983"
     CDS             323..1387
                     /gene="proW"
                     /locus_tag="QBF46_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDQ6676103.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            1445..2437
                     /gene="proX"
                     /locus_tag="QBF46_003984"
     CDS             1445..2437
                     /gene="proX"
                     /locus_tag="QBF46_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDQ6676104.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            2729..3913
                     /locus_tag="QBF46_003985"
     CDS             2729..3913
                     /locus_tag="QBF46_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6676105.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4037..4774
                     /gene="ygaZ"
                     /locus_tag="QBF46_003986"
     CDS             4037..4774
                     /gene="ygaZ"
                     /locus_tag="QBF46_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDQ6676106.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            4764..5099
                     /gene="ygaH"
                     /locus_tag="QBF46_003987"
     CDS             4764..5099
                     /gene="ygaH"
                     /locus_tag="QBF46_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDQ6676107.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALTYG
                     LVWKVMAII"
     gene            5190..5720
                     /gene="emrR"
                     /locus_tag="QBF46_003988"
     CDS             5190..5720
                     /gene="emrR"
                     /locus_tag="QBF46_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDQ6676108.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            5847..7019
                     /gene="emrA"
                     /locus_tag="QBF46_003989"
     CDS             5847..7019
                     /gene="emrA"
                     /locus_tag="QBF46_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDQ6676109.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7036..8574
                     /gene="emrB"
                     /locus_tag="QBF46_003990"
     CDS             7036..8574
                     /gene="emrB"
                     /locus_tag="QBF46_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDQ6676110.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(8638..9153)
                     /gene="luxS"
                     /locus_tag="QBF46_003991"
     CDS             complement(8638..9153)
                     /gene="luxS"
                     /locus_tag="QBF46_003991"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDQ6676111.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(9303..10859)
                     /gene="gshA"
                     /locus_tag="QBF46_003992"
     CDS             complement(9303..10859)
                     /gene="gshA"
                     /locus_tag="QBF46_003992"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDQ6676112.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(10932..11360)
                     /locus_tag="QBF46_003993"
     CDS             complement(10932..11360)
                     /locus_tag="QBF46_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6676113.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(11357..11923)
                     /gene="yqaB"
                     /locus_tag="QBF46_003994"
     CDS             complement(11357..11923)
                     /gene="yqaB"
                     /locus_tag="QBF46_003994"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDQ6676114.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         2754 a         3249 c         3158 g         3032 t
ORIGIN      
        1 cctggcttcg gcccacgttc ggcgctgaaa ttattgcagg atgaagaccg cgaatatggc
       61 tacgttatcg aacgcggtaa taagtttgtc ggcgcagttt ccatcgattc gcttaaaacc
      121 gcgttaacgc agcagcaagg tcttgatgcg gcgctgattg atgcgccgtt agcagtcgat
      181 gcacaaacgc ctcttagcga gttgctctct catgtcggac aggcaccctg tgcggtgccc
      241 gtggtcgacg aggaccaaca gtatgtcggc atcatttcga aaggaatgct gctgcgcgct
      301 ttagatcgtg agggggtaaa taatggctga tcaaaataat ccgtgggata ccacgccagc
      361 ggcggacagt gccgtgcaat ccgcagacgc ctggggtaca ccggcgactg caccgactga
      421 cggcggtggt gctgactggc tgaccagtac gcctgcgcca aacgtcgagc attttaatat
      481 tctcgatccg ttccataaaa cgctgatccc gctcgacagt tgggtcactg aagggatcga
      541 ctgggttgtt acccatttcc gtcccgtctt ccagggcgtg cgcgttccgg ttgattacat
      601 tctcaacggt ttccagcaat tgctgctggg tatgcccgca ccggtggcga ttatcgtttt
      661 cgctctcatc gcctggcaga tttccggggt cggaatgggc gtggcgacgc tggtttcgct
      721 gattgccatc ggcgcaatcg gtgcctggtc gcaggccatg gttaccctgg cgctggtgtt
      781 aaccgccctg ctgttctgta tcgtcattgg tttgccgctg gggatctggc tggcgagaag
      841 tccgcgagcg gcgaaaatta ttcgtccact gcttgatgcc atgcagacca cgccagcgtt
      901 tgtttatctg gtgccaatcg tcatgctatt tggtatcggt aacgtgccgg gcgtggtggt
      961 gacgatcatc tttgctctgc cgccgattat ccgtctgacc attctgggga ttaaccaggt
     1021 tccggcggat ctgattgaag cctcgcgctc attcggtgcc agcccgcgcc agatgctgtt
     1081 caaagttcag ttaccgctgg cgatgccgac cattatggcg ggcgttaacc agacgctgat
     1141 gctggccctt tctatggtgg tcattgcctc gatgattgcc gtcggcgggc tgggtcagat
     1201 ggtacttcgc ggtatcggtc gtctggatat ggggcttgcc accgttggcg gcgtcgggat
     1261 tgtgatcctc gccattatcc tcgatcgcct gacgcaggcc gttgggcgcg actcacgcag
     1321 tcgcggcaac cgtcgctggt acaccactgg ccctgttggt ctgctgaccc gcccattcat
     1381 taagtaactc tgcacttgcc cggtgacgcc gggcattatc accctgccaa aaaaaggaat
     1441 aacaatgcga catagcgtac tttttgcgac agcgtttgcc acgcttatct ctacacaaac
     1501 ttttgctgcc gatctgccgg gcaaaggtat tactgttaat ccagttcaaa gcaccatcac
     1561 cgaagaaacc ttccagacgc tgctggtcag ccgtgcgctg gagaaattag gctataccgt
     1621 caacaaaccc agcgaagtag attacaacgt tggctacacc tcgcttgctt ccggcgatgc
     1681 aaccttcacc gccgtgaact ggacgccact gcatgacaac atgtacgaag ctgccggtgg
     1741 cgataagaaa ttttatcgtg aaggggtatt tgttaacggc gcggcacagg gttacctgat
     1801 cgataagaaa accgccgacc agtataaaat caccaacatc gcacaactga aagatccgaa
     1861 gatcgccaaa ctgttcgata ccaacggcga cggtaaagcg gatttaaccg gttgtaaccc
     1921 tggctggggc tgcgaaggtg cgatcaacca ccagcttgcc gcgtatgaac tgaccaacac
     1981 cgtgacgcat aatcagggga actacgcagc gatgatggcc gacaccatca gtcgctacaa
     2041 agagggcaaa ccggtgtttt attacacctg gacgccgtac tgggtgagta atgagctgaa
     2101 gccagggaaa gatgtggtct ggttgcaggt gccgttctcc gcactgccgg gcgataaaaa
     2161 tgccgatacc aaactgccga atggcgcgaa ttacggcttc ccggtcagca ccatgcatat
     2221 cgttgccaac aaagcctgga ccgagaaaaa tccggcggca gccaaactgt ttgccattat
     2281 gcagttgcca gtggccgata ttaacgccca gaacgccatt atgcatgacg gcaaagcctc
     2341 agaaggcgat attcagggcc acgttgatgg ctggatcaaa gcccaccagc agcagttcga
     2401 tggctgggtg aatgaggcgc tggcagcgca gaagtaattt ttattcaggc ggataaggcg
     2461 tttttgcctc atccgccgct ctgtacacaa tgcctgatgc gacgctgacg cgtcttatca
     2521 tgcctacaaa atacactcat tccgtatggc ggataaggcg ttttcgccgc atccgccgtt
     2581 ctgtgcacaa tgcctgatgc gacgctggcg cgtcttatca tgcctacaaa ccacatcgca
     2641 catttcaaca agctatcttt catcccatat tcatcaacat ccgctattat tgatttccag
     2701 cttaatcatc acctgatgaa caaaaataat gactaaacct aatcatgagc ttagcccggc
     2761 gctgatcgtg ctgatgtcta tcgccaccgg tctggcggtc gccagtaact attacgctca
     2821 gccattgctc gacaccatcg cgcgtaactt ttccctttcc gccagttcgg caggctttat
     2881 tgttaccgcc gcgcaattgg gctatgcagc aggtctgctg tttcttgttc ccctcggtga
     2941 tatgtttgaa cgccgccgcc tgattgtctc gatgacctta ctggcggcag gcggcatgtt
     3001 aatcactgcc agcagtcagt cgctggcgat gatgatcctc ggtacggcat taaccggttt
     3061 attctctgtc gtggcacaaa ttctggttcc gctggcagcg acgctggctt caccggacaa
     3121 acgcggcaaa gtggttggca ctattatgag cggtctgctg ttggggatct tgctggcacg
     3181 gacagttgcc ggattgctgg cgaatctcgg cggctggcgc accgtctttt gggttgcttc
     3241 ggtgttaatg gcactgatgg cgctggcatt atggcgtggt ctgccacaaa tgaaatcaga
     3301 aacccacctc aactacccac agttgttggg ttccgttttc agtatgttta tcagcgataa
     3361 aattctgcgt acccgcgcgt tgctgggctg cctgaccttt gccaatttca gcattctctg
     3421 gacctcaatg gcctttttgc ttgccgctcc accttttaac tacagcgatg gtgtaattgg
     3481 tctgtttgga cttgcgggag ctgccggagc gttgggcgct cgtccggcgg gcggttttgc
     3541 cgataagggc aaatcgcacc acaccacaac tttcggtctg ctgctgctat tactttcatg
     3601 gctggcgatc tggtttggtc acacttccgt actggcgttg attatcggaa tcctggtgct
     3661 ggatctcacc gtgcagggcg tgcatatcac taaccagacg gtaatttatc ggatacatcc
     3721 tgatgcgcgt aatcgcctga ccgcaggtta catgactagc tactttattg gcggtgccgc
     3781 cggttcgcta atttcagcct cagcctggca acatggcggt tgggctggcg tttgtctggc
     3841 tggcgcgacg attgccctgg ttaacttact ggtctggtgg cgaggttttc atcgtcagga
     3901 agccgcaaat taagcaatca gcaaccgttt tcgtggcttt acacttataa gggtgttaag
     3961 aagcccatca gtctgataag gttaagatat tcattcagtc tatttataat attaacaatc
     4021 gttaagcgta cactctatgg aaagccctac tccacagcct gctcctggtt cggcgacctt
     4081 catggaagga tgcaaagaca gtttaccgat tgttattagt tatattccgg tggcctttgc
     4141 gttcggtctg aatgcgaccc gtctgggatt ctctcctctc gaaagcgttt ttttctcctg
     4201 catcatttat gcaggcgcga gccagttcgt cattaccgcg atgctggcag ccgggagtag
     4261 tttgtgggtt gctgcactga ccgtcatggc aatggatgtt cgccatgtgt tgtatggccc
     4321 gtcactgcgt agccgtatta ttcagcgtct gcaaaaatcg aaaaccgccc tgtgggcgtt
     4381 tggcctgacg gatgaggttt ttgccgccgc aaccgcaaaa ctggtacgca ataatcgccg
     4441 ctggagcgag aactggatga tcggcattgc cttcagttca tggtcatcgt gggtatttgg
     4501 tacggtaata ggggcattct ccggcagcgg cttgctgcaa ggttatcccg ccgttgaagc
     4561 agcattaggt tttatgcttc cggcactctt tatgagtttc ctgctcgcct ctttccagcg
     4621 caaacaatct ctttgcgtta ccgcagcgtt agttggtgcc cttgcaggcg taacgctatt
     4681 ttctattccc gtcgccattc tggcaggcat tgtctgtggc tgcctcactg cgttaatcca
     4741 ggcattctgg caaggagcgc ccgatgagct atgaggttct gctgcttggg ttactggttg
     4801 gcgcggcgaa ttattgcttc cgctatttgc cgctgcgcct gcgtgtgggt aatgcccgcc
     4861 caaccaaacg tggcgcggta ggtattttgc tcgacaccat tggcatcgcc tcgatatgcg
     4921 ctctgctggt tgtctctacc gcaccagaag tgatgcacga tacacgccgt ttcgtgccca
     4981 cgctggtcgg cttcgcggta ctgggtgcca gtttctataa aacacgcagc attatcatcc
     5041 caacactgct tagtgcgctg acctacgggc tcgtctggaa agtgatggcg attatataac
     5101 ccacaagaat catttttcta aaacaataca tttactttat ttgtcactgt cgttactata
     5161 tcggctgaaa ttaatgaggt catacccaaa tggatagttc gtttacgccc attgaacaaa
     5221 tgctaaaatt tcgcgccagc cgccacgaag attttcctta tcaggagatc cttctgactc
     5281 gtctttgcat gcacatgcaa agcaagctgc tggagaaccg caataaaatg ctgaaggctc
     5341 aggggattaa cgagacgttg tttatggcgt tgattacgct ggagtctcag gaaaaccaca
     5401 gtattcagcc ttctgaatta agttgtgctc ttggatcatc ccgtaccaac gcgacgcgta
     5461 ttgccgatga actggaaaaa cgcggttgga tcgaacgtcg tgaaagcgat aacgatcgcc
     5521 gctgcctgca tctgcaatta acggaaaaag gtcacgagtt tttgcgcgag gttttaccac
     5581 cgcagcataa ctgcctgcat caactctggt ccgcgctcag cacaacagaa aaagatcagc
     5641 tcgagcaaat cacccgcaaa ttgctctccc gtctcgacca gatggaacaa gacggtgtgg
     5701 ttctcgaagc gatgagctaa cgcgtcatct cgctcaaaaa tccagattta taaaagaaaa
     5761 aatgactggc cagcatcgca acatgctggc ctttttggca agcaggtcgg ctcagccgat
     5821 gagttaagaa gatcgtggag aacaatatga gcgcaaatgc ggagactcaa accccgcagc
     5881 aaccggtaaa gaagagcggc aaacgtaagc gtctgctcct ccttctcacc ttgctcttta
     5941 taattattgc cgtagcgata ggcatttatt ggtttttggt actgcgtcac ttcgaagaaa
     6001 ccgatgacgc atacgtggca gggaatcaaa ttcaaattat gtctcaggtg tctggcagcg
     6061 tgacgaaagt ctgggccgat aacaccgatt ttgtaaaaga aggcgacgtg ctggtcactc
     6121 tcgacccgac agatgctcgc caggcgtttg aaaaagccaa aactgcactg gcttccagcg
     6181 ttcgccaaac ccaccagctg atgattaaca gcaagcagtt gcaggcgaat attgaggtgc
     6241 agaaaatcgc cctcgcgaaa gcacaaagcg actacaaccg ccgtgtgccg ctgggcaatg
     6301 ccaacctgat tggtcgcgaa gagctgcaac acgcccgcga cgccgtcacc agtgcccagg
     6361 cgcaactgga cgtcgcgatt caacaataca atgccaatca ggcgatgatt ctggggacta
     6421 aactggaaga tcagccagcc gtgcaacagg ctgccaccga agtacgtaac gcctggctgg
     6481 cgctggagcg tactcgtatt gtcagtccga tgaccggtta tgtctcccgc cgcgcggtac
     6541 agcctggggc gcaaattagc ccaacgacgc cgctgatggc ggtcgttcca gccaccaata
     6601 tgtgggtgga tgccaacttt aaagagacgc agattgccaa tatgcgtatc ggtcagccgg
     6661 tcactatcac cacggatatt tacggcgatg atgtgaaata caccggtaaa gtggttggtc
     6721 tggatatggg cacaggtagc gcgttctcac tgcttccagc gcaaaatgcg accggtaact
     6781 ggatcaaagt cgttcagcgt ctgcctgtgc gtatcgaact ggaccagaaa cagctggagc
     6841 aatatccgct gcgtatcggt ttgtccacgc tggtgagcgt caataccact aaccgtgacg
     6901 gtcaggtact ggcaaataaa gtacgttcca ctccggtagc ggtaagcacc gcgcgtgaaa
     6961 tcagcctggc acctgtcaat aaactgatcg acgatatcgt aaaagctaac gctggctaat
     7021 ccagaggtgc gtgtgatgca acagcaaaaa ccgctggaag gcgcgcaact ggtcattatg
     7081 acgattgcgc tgtcactggc gacattcatg caggtgctgg actccaccat tgctaacgtg
     7141 gcgatcccca ctatcgccgg gaatctgggc tcatcgctca gccagggaac gtgggtaatc
     7201 acttctttcg gggtggcgaa tgccatctcg atcccgctta ccggctggct ggcaaagcgc
     7261 gtcggggaag tgaaactgtt cctttggtcc accatcgcct ttgctattgc gtcgtgggcg
     7321 tgtggtgtct ccagcagcct gaatatgctg atcttcttcc gcgtgattca ggggattgtc
     7381 gccgggccgt tgatcccgct ttcgcaaagt ctattgctga ataactaccc gccagccaaa
     7441 cgctcgatcg cgctggcgtt gtggtcgatg acggtgattg tcgcgccaat ttgcggcccg
     7501 atcctcggcg gttatatcag cgataattac cactggggct ggatattctt catcaacgtg
     7561 ccgattggcg tggcggtggt gttgatgaca ctgcaaactc tgcgcggacg tgaaacccgc
     7621 accgaacggc ggcggattga tgccgtgggg ctggcactgc tggttattgg tatcggcagc
     7681 ctgcagatta tgctcgaccg cggtaaagag ctggactggt tttcatcaca ggaaattatc
     7741 atccttaccg tggtggcggt ggtggctatc tgcttcctga ttgtctggga gctgaccgac
     7801 gataacccga tagtcgatct gtcgttgttt aagtcgcgca acttcaccat cggctgcttg
     7861 tgtatcagcc tcgcgtatat gctctacttc ggcgctattg ttctgctgcc gcagttgttg
     7921 caggaggtct acggttacac ggcgacctgg gcaggtttgg cctctgcgcc ggtagggatt
     7981 attccggtga tcctgtcgcc gattatcggc cgcttcgcgc ataaactgga tatgcggcgg
     8041 ctggtaacct tcagctttat tatgtatgcc gtctgcttct actggcgtgc ctataccttt
     8101 gaaccaggta tggattttgg cgcgtcggcc tggccgcagt ttatccaggg gtttgcggtg
     8161 gcctgcttct ttatgccgct gaccaccatt acgctgtctg gtttgccacc ggaacgactg
     8221 gcggcggcat cgagcctctc taactttacg cgaacgctgg cggggtctat cggcacgtcg
     8281 ataaccacga ccatgtggac caaccgcgag tcgatgcacc atgcgcagtt gactgagtcg
     8341 gtaaacccgt tcaacccgaa tgcccaggcg atgtacagtc aactggaagg gcttgggatg
     8401 acgcaacagc aggcatcagg ctggattgcc cagcagatca ccaatcaggg gctgattatt
     8461 tccgccaatg agatcttctg gatgtcagcc gggatattcc tcgtcctgct ggggctggtg
     8521 tggtttgcta aaccgccatt tggcgcaggt ggcggcggag gcggtgcgca ctaagtacaa
     8581 ctaagccagt tcatttgaac tggctttttt caattaattg tgaagatagt ttactgacta
     8641 gatgtgcagt tcctgcaact tctctttcgg cagtgccagt tcttcgttgc tgttgatgcg
     8701 tacgtcacgt tccagaatgc tacgcgcaat atcctgcgct tcctgcaacg agtgcatctg
     8761 gtaagtgcca cactggtaga cgttcagttc cgggatctga ttctgatcct gcactttcag
     8821 cacgtcttcc attgccgctt tccaggcatc agcaacacgc tgctcatctg gcgtaccaat
     8881 cagactcata taaaaaccgg tgcggcagcc cattggcgag atatcgataa tctctacacc
     8941 attaccgtta agatggttac gcataaaacc agcaaacagg tgctccaggg tatggatccc
     9001 tctttctggc atcacttctt tgttcggcac gcagaagcgc agatcgaaca cggtgattgc
     9061 gtcgccatgc ggggtgttca ttgttttcgc cacccgaact gcaggcgctt ccatccgggt
     9121 atgatcgact gtgaagctat ctaacaacgg catttagcca cctccggtaa tttttttaaa
     9181 aattttctga actctttctt cccaggcgag tctgagtata tgaaagacgc gcatttgtta
     9241 tcatcatccc tgaattcaga gatgaaattt tggccactca cgagtggcct ttttcttttc
     9301 tgtcaggcgt gtttttccag ccacaccgca aacggttcgg tatcagcggc ttccatttcc
     9361 tgctgacggc gttcagacgc ctcgcgctcg gctacaaaat cctcttcgcg cagaatttcc
     9421 agcggctctt cacgcagcag attacggtag gcttctgcaa atgctttgcc tgttccgcca
     9481 ataccagtat caatcataga ccttaagata cgggcagaga aagtcagatc gggattatcg
     9541 aagcaggcaa ccagttcatc acacactttc tgatacgctt cgccgccgtt aatactatcc
     9601 agcgtttgcg cgacgcgttt cagatcgcgg aacagatctt tacccacctg cggtaacggg
     9661 aactgtgcgg tttcgcagcc gatacccagc gtcagacccg gtttgcgacc ttcgaggatc
     9721 acccggttcc agttaacgcg tgtacaggca agttcgctac tgctcatttc cggtgcatca
     9781 gccagcgcac accagaccat aaacaggtcg aggaatcgca cctgctgttc atctacacca
     9841 atcggcgaga acgggttgat gtccagcgaa cgcacttcaa tatattcaat gccgccacgt
     9901 aacagcgcat cagaaggcga ctcgccgctg cgggtaacgc gttttggacg aatcggcgcg
     9961 tacagttcgt tttcaatctg caacacgttg ctgttgattt gcagcctctt accgtctttc
    10021 tcaataccaa tcttcgcgta ctcttccgat ggcgttttga ttgcctgttt aaggcccgct
    10081 acgtactcgt aaagatcgtt gaaggtaata ccaagattgc tttgcgattt attggtatag
    10141 ccgagatcgc tcaaacgaag agaggtcgca tacggcaggt aatacatacc gcactcggtt
    10201 ttctcaaacg gcagcgacgt tggttttcct tgcaggaaag aagaacaaat cgccggagat
    10261 gcaccaaaca gataaggaat gacccaaccg aaacgatagt aattgcggat aacgcggaaa
    10321 tagcccgcag aaattttctc tttggcatca gcgcccgaga tatcaccgca cttcgcttgc
    10381 cagaatgcca ttggcaaaga gaaattgtag tgcacgccgg aaatggtttg catcagcgcg
    10441 ccgtagcgat ttttcagccc ttcacgatac agcgttttaa agcgtccggt gttagaagtg
    10501 ccgtactgtg ccagttcgat gtcctgacct tctgcgatgt agcatggcat acttaacggc
    10561 cacatccgct catcgcccat attgcgcgcc gtataacgat gcagatcgcg cataaaggtc
    10621 agcatatgtt caatatcacc atccactggt gtaatgaatt ccagcaatgc ttccgcaaaa
    10681 tcggtagtaa tccatttgtg cgtcagtgcg gaacctaatg cttcaggatg acctgttgtt
    10741 gccagtgtgc catcagcatt aacacgcaaa gtttcgcgct ccagcccacg ctgtatcccc
    10801 tttaacgcct gaggatgttt ttccagccag gccagcgcct gtgatacgtc cgggatcaaa
    10861 ttgacctccc gcctgtcaaa atcgttttaa ttagcataac tgtaatggtg accatatgtg
    10921 caggcctaca attagtgcca ccacatcatg ccctgaacgg tcgctgctgc aactgcaaca
    10981 tagcgtaacg ctttaccaag gcacaaaaaa aagattaccg gtccccacga gatgcgcatc
    11041 catcccgcta acagacacag taaatcgcca accacgggca tccagcttaa taatagcgtg
    11101 actgcaccat agcgtttcag ccagccggta gctttctctt gccagcgcga tgttttacgc
    11161 aatggaaaga aacgcccaag gataacgtta gttaaccctc caaggctatt acccattgtt
    11221 gctgttaaaa ctaaaaccca gggatgactg atcccggaaa gcaacattgc caccagcacg
    11281 acttcggagt tgccgggtaa tagtgtagcg ctgagaaaac tactggcaaa caacgagaaa
    11341 agcgataacg cttcactcac agcaagcgaa catccacggc gtccatgcct gctgcacggg
    11401 ccgcctgaat accgaaatcg gcatcttcaa agaccacaca ctgcgtcggt tgcacgccca
    11461 tacgctgcgc gcacaacaaa aatgtgtctg gcgcgggttt atggtgtttg acgtgatcgg
    11521 cagcgacgac ggcgtcaaaa taatggcgta atcccaggtg cgccagcaat gcctcagcga
    11581 tggcgctttc actccccgtt cctacagcca ttgggcgacg accatgccaa cttttcacca
    11641 catcaacaag aggaagcggt tcgacgctat ccagcagcat acttcttact gcttctgttt
    11701 tttcacgcgc taacgcatgc gggtcgagat cggcctgatt cagctcaata attgcctgag
    11761 caatacgcca ggtgggcgat ccattaagcg caatcatcgc ctgaatatcg tactgaagac
    11821 cgtagtgccc taatacttcg cgccacgctt tacggtgcgt aggctccgta tccaggattg
    11881 tgccatccat atcaaaaatt aaacctgcat aacgctcgta catggtcttc tcgcaagtcg
    11941 aaaaatatga cgctacttta gcgtaattgc ttgattttgt cgctgatggc gaaggagaag
    12001 aaaattcagg aaatggataa aggagttatt tagaaatgag atatttttga aggaaatttt
    12061 ttggagaaga tggtgcatcc gggaggatga ctcactgacg ttcgcccttc gggccgttgc
    12121 tggcgcaacg ttatccttcc tggtgcctgc ggttaatacc gctatctctt cgataccttc
    12181 attgctgaag gtt
//
LOCUS       DAOFQD010000141        11915 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.141, whole genome shotgun sequence.
ACCESSION   DAOFQD010000141 DAOFQD010000000
VERSION     DAOFQD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.141"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            121..618
                     /gene="ftnA"
                     /locus_tag="QBF46_003995"
     CDS             121..618
                     /gene="ftnA"
                     /locus_tag="QBF46_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDQ6676115.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(656..895)
                     /locus_tag="QBF46_003996"
     CDS             complement(656..895)
                     /locus_tag="QBF46_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDQ6676116.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1086..2297
                     /gene="tyrP"
                     /locus_tag="QBF46_003997"
     CDS             1086..2297
                     /gene="tyrP"
                     /locus_tag="QBF46_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDQ6676117.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGVVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2359..3024)
                     /gene="yecA"
                     /locus_tag="QBF46_003998"
     CDS             complement(2359..3024)
                     /gene="yecA"
                     /locus_tag="QBF46_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDQ6676118.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3220..3306)
                     /locus_tag="QBF46_003999"
     tRNA            complement(3220..3306)
                     /locus_tag="QBF46_003999"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3270..3272),aa:Leu,seq:taa)
     gene            complement(3319..3392)
                     /locus_tag="QBF46_004000"
     tRNA            complement(3319..3392)
                     /locus_tag="QBF46_004000"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3358..3360),aa:Cys,seq:gca)
     gene            complement(3447..3522)
                     /locus_tag="QBF46_004001"
     tRNA            complement(3447..3522)
                     /locus_tag="QBF46_004001"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3487..3489),aa:Gly,seq:gcc)
     gene            complement(3674..4222)
                     /gene="pgsA"
                     /locus_tag="QBF46_004002"
     CDS             complement(3674..4222)
                     /gene="pgsA"
                     /locus_tag="QBF46_004002"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDQ6676119.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4279..6111)
                     /gene="uvrC"
                     /locus_tag="QBF46_004003"
     CDS             complement(4279..6111)
                     /gene="uvrC"
                     /locus_tag="QBF46_004003"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDQ6676120.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKRYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6108..6764)
                     /gene="uvrY"
                     /locus_tag="QBF46_004004"
     CDS             complement(6108..6764)
                     /gene="uvrY"
                     /locus_tag="QBF46_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDQ6676121.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7060..7236
                     /locus_tag="QBF46_004005"
     CDS             7060..7236
                     /locus_tag="QBF46_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676122.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7223..7447
                     /locus_tag="QBF46_004006"
     CDS             7223..7447
                     /locus_tag="QBF46_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDQ6676123.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7514..8236)
                     /gene="sdiA"
                     /locus_tag="QBF46_004007"
                     /pseudo
     CDS             complement(7514..8236)
                     /gene="sdiA"
                     /locus_tag="QBF46_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416426.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(8466..9218)
                     /gene="yecC"
                     /locus_tag="QBF46_004008"
     CDS             complement(8466..9218)
                     /gene="yecC"
                     /locus_tag="QBF46_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDQ6676124.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(9215..9883)
                     /gene="tcyL"
                     /locus_tag="QBF46_004009"
     CDS             complement(9215..9883)
                     /gene="tcyL"
                     /locus_tag="QBF46_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDQ6676125.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9898..10884)
                     /gene="dcyD"
                     /locus_tag="QBF46_004010"
     CDS             complement(9898..10884)
                     /gene="dcyD"
                     /locus_tag="QBF46_004010"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDQ6676126.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10989..11789)
                     /gene="tcyJ"
                     /locus_tag="QBF46_004011"
     CDS             complement(10989..11789)
                     /gene="tcyJ"
                     /locus_tag="QBF46_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6676127.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT         2941 a         3117 c         2780 g         3077 t
ORIGIN      
        1 gcgttttatt cccattcgta tttttaataa ttgaagttta tattttacct tttgcaaata
       61 ataaaataac aaattatagt gacgccacag caacaaatat aacctttgtg gagcactatc
      121 atgctgaaac cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct
      181 tcactgcttt atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc
      241 gcgttcctgc gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac
      301 ctgactgata ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat
      361 tcctcacttg atgaattatt ccaggaaacc tataaacacg aacaattaat cacccagaaa
      421 attaacgaac tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg
      481 caatggtatg tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa
      541 ttaagcctgg caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc
      601 ctcgacacac aaaactaatg ctctccggcg gcaggtttac tgccgccgtt tcaaatcagt
      661 gacggcaaat cttactctga tcggtcgaaa aaccgtcttt cgaaggtata aatttgcctt
      721 ttgctgccag aaacgctacc agctctcccg ctgtcatccc ttctgccgag caggtgtgaa
      781 aacgtgcctg ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca tgcgtatatt
      841 gctcgcctga ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgtcc
      901 ctcctcaaaa aaagcctagc gtagcgattg ccgcttatga agactttgcg ccagcgcagg
      961 actgaatgct ttttattgta catttatatt tacaccatat gtaacgtcgg tttgacgaag
     1021 cagccgttat gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag
     1081 aaagcgtgaa aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc acaattggcg
     1141 caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt acgttaatct
     1201 tgttgattgg gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc
     1261 agcatgttcc ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg
     1321 gtcaatggct gacgggcttc agtatgatgt tcttaatgta tgctctgact gcggcataca
     1381 tcagcggtgc cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt
     1441 cggcaaccgc tggcgtgctg ttgttcactt ttgttgccgg tgtcgtggtt tgtgttggaa
     1501 catcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg
     1561 tgatgctggt attgctacta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc
     1621 aacaggggct ggctctgtca gcaatcccgg tgatttttac ctcatttggt tttcacggta
     1681 gcgtgccgag tattgtcagc tatatggatg gcaacgttcg taagctacgc tgggtgttta
     1741 taaccggtag tgcgatcccc ctggtggcat atattttctg gcaggtggca acgcttggca
     1801 gcattgattc aacaaccttt atgggattgc tggctaatca tgctggatta aacgggctgt
     1861 tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcagtg catttatttg
     1921 ctgatttagc cctcgccacg tcatttctcg gcgttgcgtt aggcttattt gattatctgg
     1981 ctgatttatt tcagcgttca aataccgttg gtggacggtt gcaaactggt gcaattacct
     2041 ttctgccgcc gttggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt
     2101 acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcgctgttg acctggcaaa
     2161 gcagaaagca caatcctcag gcgggttacc gggtgaaagg tggtcgtccg gcgctggtgg
     2221 tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcagggttgt
     2281 taccagaagt ggggtgatca gatagcctca aattccttat tgggtgccag aattaacgct
     2341 gacacccaat ttgacctctt aatgcaggca gcactgctta aatttcttac cactaccgca
     2401 cgggcaagga tcgttacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt
     2461 ttcctgcgga tgcgccatcc agtacgcatg tagatcaagc gccgccagtc gaatggcatc
     2521 tacgctctct tcaaacgctt ctggcgacat tttctctacc cgctcgaagt tttcctcagt
     2581 accgtgcagc gcaatcgcct ccagcgctgg ttttaacgaa tcgggcaacg ttgaccagtc
     2641 agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct
     2701 gccatcaact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg
     2761 ctctgcggta tcggccatat gttgaaaagc cagattcata aagcgcgtca tctctttctc
     2821 tgacgcccag cgcggcacat agtcagcccc accccacacg gcaaccagcc actgtgccgg
     2881 ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc
     2941 aaggatggcg tggtcagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact
     3001 ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc gccagcacct
     3061 atcttacatg ccggtccgta tcagagatac tttttgagtg gctttgctgg tgattaaaaa
     3121 ttaaggaggg tgtaacgaca agtttcaggc acaaaaaaac cacccgaagg tggtttcacg
     3181 acactgctta ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg
     3241 aacccgcaca gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat
     3301 ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc
     3361 aatccgctac ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat
     3421 ccgcttgagg ccgatgtctt ttaaactgga gcgggaaacg agactcgaac tcgcgacccc
     3481 gaccttggca aggtcgtgct ctaccaactg agctattccc gcattcatca agcaatcagt
     3541 taatcacttg attttattat cgtctggcaa tcagtgccgc cgttcgatgc gttgcattct
     3601 acttacctgg cgcgatgagt caacgatatt tttcaccact tttgatcgtt tgctgaaaat
     3661 tacgccgaaa cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa tattgcaaca
     3721 ttgaccacag agtcagtacc gcagccacaa agaaaagtgc aataccggcg tactcaaccc
     3781 aaatgttcgg acgccacagc agccaggcca acgccaccat ctgggcagtg gttttcactt
     3841 tcccaatcca ggagacagcc acgctactgc gtttacccaa ctccgccatc cattcgcgta
     3901 gcgcagaaat aataatttca cgcgcgatca tcgttgccgc cggtagggtc acccaccagc
     3961 tgtgataatg ctcggttacc agcaccatgg cgatagccac gagaacttta tctgctacag
     4021 ggtcaaggaa agcaccaaac cgggtactct ggttccagcg gcgtgccaga aaaccatcga
     4081 accagtcagt caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc gaccaggtga
     4141 caggcagata aaagaccaat acaaagaatg ggataaggat gacacggaac agtgtaagca
     4201 acgtagggat attaaattgc ataatgacgg gtaactatct gttgtcagta agattacccc
     4261 tatgttgcta cagagacatc aatgtttcaa cgaccagaag atcttttctg ccagaccttg
     4321 cgaaataccc ggcacttttg caatttcctc gacgctggcg ttacgtaaac cttgcaaacc
     4381 gcccatatat ttcaacaaca tttgccgacg ttttggcccg acgccttcaa tggtttccag
     4441 ggaactggta tttttgactt tcgcccgttt tttacggtgc ccgccaatcg cgtgatcatg
     4501 tgattcatcg cgaatatgct ggataacatg cagcgcgggt gaatctggcg gcaaactaaa
     4561 tccctcacct tccggctcaa agaacagcgt ttccagtcca gccttacgat ctgctccttt
     4621 ggcaacgcca agtagcagcg gatgattttt atcccatgag acatccagtt cggcgaagac
     4681 atttttcgcc tgcgcaagct ggcctttgcc gccgtcgata aggatcacat ccgggatctt
     4741 actgtcgtca atggctttac cataacgccg acgcagcacc tgattcatcg ccgcataatc
     4801 atcgcccggc gtaatgccag taatgttata gcgccgatac tccgcacgca gcgggccgtt
     4861 agcatcaaac accacacagg aggcgacggt ttgttcaccc atggtatggc tgatgtcaaa
     4921 gcactccatc cgcttcactt ccggcaattt caacacgctg gcaagcgcgg tcagccgctg
     4981 gtgaacggta gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat tggtgcgcgc
     5041 gagtttcaga taacgcgccc gatcgccgcg aggtttggtt tgaacattaa tcttgcgtcc
     5101 cgccagttct gaaagggaat cggcgagcag cgttttatcg ctaagattaa aatcgagcag
     5161 gatctcaccc ggtaaggtgc gcatctggct gccttgtaaa tagaactgac ctacgaaggt
     5221 ttctaccacc tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc tgcggctgcc
     5281 gagcactttg ccctgacgaa tgaacaatac gtggacacaa gccatgcccg catcgaacgc
     5341 cacaccaata acgtcgaggt cgtcgccggt attggaaacg aattgttttt cggtgacgcg
     5401 tcgcaccgcc tgaatttggt cgcgaatacg tgccgcttct tcaaactcca gattctggct
     5461 ggcagtttcc atacgactaa tgagttgcgt aagcacctga tcatctttgc cagacaaaaa
     5521 caggcgcaca tactcgacct gctgagcgta ttcttcttca ctcaccagtc cttcaacgca
     5581 cggtcccaga cagcgcccta tctgatattg cagacacgga cgcgagcgat tgcgataaac
     5641 actattttcg cactggcgaa tggggaaaat cttttgcagt agcgccagtg tttcacgtac
     5701 ggcatagcca ttcgggaacg ggccgaaata ttcacctttg gcatgcttcg caccacgatg
     5761 catcgccaga cgcgggtggg tatcgccact caggaagata aaaggataag atttatcatc
     5821 gcgtagcaaa acgttgtaac gcggctgata gcgtttgatg tagttgtgtt ccagcaacag
     5881 cgcttcggtt tctgtatgag taaccgttac atcaatttgc tggatctggg cgaccagcgc
     5941 ttcggttttg cgcgaagcga ggttgctacg gaaatagctg gaaagccgtt ttttcaggtc
     6001 tttcgctttg ccgacataga taaccgtacc accagcatcg tacatgcgat aaacgcctgg
     6061 ctggctggtt acggttttta aaaacgcttt tgcgtcaaac tgatcactca ctgacttgat
     6121 aatgtctccg cattacacag accatggcga attgccaggt gagtcagctc aacatcgcca
     6181 tgaatgttta gtttactgaa catacgatag cggtagctgt tcaccgtttt cggactgaga
     6241 ttgagctgtt ctgagatctc attgaccttc tggcccttgg tgatcatcag cataatctgc
     6301 aattcacgtt cagacaaact ggcaaatggg ctttctgttt tttctggttc gatctggctt
     6361 aacgccattt gttgagcgat gtcagaagca atgtaacgct gccctgaata gacagaacga
     6421 atcgcactca cgacttcctg cggagccgcg cctttgctga ggtagcccgc agcaccggcc
     6481 tgcatgactt tcgctggtaa agggttttct gtatggacgg taagcatgat gattttgaca
     6541 tcagctgtgg aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc cggcatactc
     6601 atgtccatta gcaccacgtc aacggcattt gtccggcacc acttaacggc gtcttcaccg
     6661 cacgatgcct caccgacgac ttttataccc tttatatctt ccagaatgcg tcgtatccct
     6721 gcgcgcacca gttcgtggtc atcaacaagt agaacgttga tcaaaggaat atctccagaa
     6781 atagggataa cgctactgat agttagtcat tcgtatatta gcggttttta ttgcaacttt
     6841 gaaacgttaa aaatggtggg tattcgattt ttctctcgtt tttgtcaatt cgattgtcca
     6901 cagttatggt cacgcccgtc accaacggct taccggatcg tttcacagta agccctacga
     6961 tgaaatgtga caaaaattga ctttattcag caaaaatgaa aatcagcccg ctgaatatgt
     7021 aacattaatt aacccgaaca atacgggcaa aaaacattca tgataaaaat atttattggt
     7081 cattatatta acgtatttta tagcactgcc gatatcacgc tcaaaaaaca accactgcta
     7141 tttttagcaa agcttatggt atactccgcc gccttaacat ttttcaccgc aaattttcat
     7201 tgcaacatga cgaggaaaat aaatgagtac gcctgatttt tctactgccg agaataatca
     7261 agaactggca aatgaagtct cctgcctgaa agcgatgctg acgctgatgc tgcaggcgat
     7321 gggacaagct gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg cgctgatcga
     7381 agacgaaacc caggctgcag tattttccaa aacggttaag caaattaaac aagcctaccg
     7441 tcagtaatat aaaaccggct gagagcgtgc ctttcagccg gtttttgcat ctggcacgca
     7501 gaaagaaaag cgatcaaatt aagccagtag cggccgcgta acaggccacc tgggtcttat
     7561 ttggtgcatt gatttttttc tgcatgtttt tctgatggaa attgaccgta ttctcagaga
     7621 ttgacaaaat catcgctatc tctgctgatg tcttcccttc cgccgtccat ttcaaaattt
     7681 ctttttcgcg cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca
     7741 tcagagccat cagactttcg cgcaccagta actgcatttt taattgcagt tcatcactaa
     7801 gaatgggtat ttcgcgcgcg ctgctacggg aaaaggacaa aaagcccagc gcccggtttg
     7861 gcagcattaa atactgagtg acaccgcggc gtaaaccatg tgcgcgcgcg gcttcccata
     7921 acggctgtgc ttcgctgaat aagtcatcat tccacattaa atggccctga ctaaagtttt
     7981 cagggttcag caccggatca attgcgagaa agttttttgc ctgataataa ctaacccacg
     8041 cctcagggta attggtgtag aaagccactt taggtcgcgt gaatggtacc gggtggcgaa
     8101 cacataacga atagtaatcg tactccagct actgagcctg aaactctatt tcatggtaga
     8161 cctcttctgc ggcctccatc ctctgaaaac gcaacagcat cgtgcgacgc cagctgaaaa
     8221 aatccttatc ctgcatagta aaccgcaacg cccctgagag tgagtatcat ttataatgat
     8281 aattcaaact atcacataaa taagatttat atataattta tattattcag gcaatgaatt
     8341 acttttgcaa gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca
     8401 gttttatcag gcttgaaatg gcgtccagcc ccgacaggtg aatcgtcggg gctgattttt
     8461 tcttattatt gcagcagaaa cttctcgagg aactggcggg tgcgaggctg ctcgggatcg
     8521 gcaaataacg cttttgcggc cccctgctcg actatccgcc cctggtccat aaagatcgcc
     8581 cggtccgcaa catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt
     8641 tcctgcgcca gctgacggat ggtgttcagg acttcgccca ccagctctgg atccagcgcc
     8701 gacgttggct cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg tgcaatcgca
     8761 acacgttgct gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc
     8821 ccaactttcg ccaacagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg
     8881 acgatcaccg gcccttcaat aatgttttcc agcaccgtac gatgcggaaa caaattaaag
     8941 ttctggaaga caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt
     9001 aatgaacgtg cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttccggttgt
     9061 tccagcagat ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt
     9121 gccaccactt cgccaggctt tacctcgagg tcgataccgt gcagcaccgt ctgaccgtga
     9181 aattttttca ccaggttctt aacttcaatg gcactcattt tggttctctc tcctggcgat
     9241 taagttgatt ctcaaaatgg ttctgcaacg tcgataatac tgtcgccatg atccagtaga
     9301 tgagcgaagc cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg
     9361 cctggcggaa cagctccggc acctggattg tcgcggccag cgacgtatct tttaccagac
     9421 tgataaagct gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc
     9481 gacgcatggt ctgccacggc gtcataccaa tactggcagc ggcttcccac tgacctttat
     9541 caatagaaga aatggcggcg cgcagcgttt cggcggcata ggcggcagtg ttcagcgaca
     9601 gaccaatcat tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat
     9661 agatcataaa cagttgtgcg atgagcggcg taccacgaaa aatagagata taaaaacgcg
     9721 ccagccagcg cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagta
     9781 gtaagccaaa aaacatgccg ccgatactga gttgcagggt atacccagcc cctttcaaca
     9841 ggaacggcaa ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta
     9901 aacgtgggga tgataggcga acagcgcagg cgcgccgccg gtatgaataa acagaatcgg
     9961 cccttcatct ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc
    10021 ggtatacaca ggatcaagca gaatgccttc cagacgcgcc agcaatttca ctgcttccat
    10081 gccttcgtcg ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc
    10141 cgctgatgcg gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg
    10201 caattgatcg gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag
    10261 gtgttccagc ccaacagcca atccggcgtg agttccggca ctgcccgatg cgaccaccac
    10321 cgacgaaata ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata
    10381 acccagcgcg cccagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg
    10441 tgcttcgact cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcgtcgca
    10501 catttcaatc tgggtattga acagatccag caacaaacga ttgccgttgg ttaaatagtt
    10561 ttctgcggtt gtgccaatag gattttccag cagcgccacg cagtgcagac cgagtttcgc
    10621 cgcaactgcg gcagtctggc gcacatggtt agactggatc gcgccggcag taatcagcgt
    10681 atcggcacct tcacgcagag catctgctgc gagaaattcc agcttacgta atttattgcc
    10741 gcccattgcc atgggggtga cgtcatcccg tttgatgaaa atttcccgtc ctagataatc
    10801 agaaaagcgc ggcagatatt cgagcggcgt tggcgcgccg ataaactcca accgtggaaa
    10861 acgggttaaa ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat
    10921 tatgcacgct gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat
    10981 tatgctgatt atttggtcac atcagcacca aaccattttt cggaaagagc ttgcagagtg
    11041 ccgtcttttt gcatttccgc aattgcatca ttcactgctt tcagcaggtc ttcatttcct
    11101 ttacgcagcg ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta
    11161 tcgttggttt tcttcaccag atccagcgcc gccagacggt caacgaggat cgcatcgata
    11221 cggcctacgc gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgacg
    11281 ccctgaacat tctgacgcag ccactcttca tagttggtgc ccagaccgac acccactttt
    11341 ttgcctttca gatcagcagc cgttttaatg gtgccttcgt tacctttttt caccagcgcc
    11401 tgaataccag aaatggtgta cggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa
    11461 atggtgacct ggttaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg
    11521 tcccatttgg tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca
    11581 aattccactt caaaaccggt taatttgccg tcatccccct gaaaactgaa cggcggataa
    11641 gtgccttcca gccctaccag cagcgtgccg cgctctttga ctttattaag cagaccttca
    11701 tctgcaaaac ttttaacgct catgcccgca accagcgcca cggccatcac acccatcaat
    11761 gcctgacgtc ccagatgtgc taatttcata ttcaccccga atgttgttat gtctgtttgc
    11821 agtgtagagc cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa
    11881 tatatatcag aagcaggctg gctggaggat ttctg
//
LOCUS       DAOFQD010000142        11913 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.142, whole genome shotgun sequence.
ACCESSION   DAOFQD010000142 DAOFQD010000000
VERSION     DAOFQD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.142"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(34..315)
                     /locus_tag="QBF46_004012"
     CDS             complement(34..315)
                     /locus_tag="QBF46_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDQ6676128.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(578..1687)
                     /gene="apbC"
                     /locus_tag="QBF46_004013"
     CDS             complement(578..1687)
                     /gene="apbC"
                     /locus_tag="QBF46_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDQ6676129.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1819..3852
                     /gene="metG"
                     /locus_tag="QBF46_004014"
     CDS             1819..3852
                     /gene="metG"
                     /locus_tag="QBF46_004014"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDQ6676130.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLANELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3993..7787
                     /locus_tag="QBF46_004015"
     CDS             3993..7787
                     /locus_tag="QBF46_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDQ6676131.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIEWDYANNVIIILPSGIAPSYLEQYSRFELR
                     LRKHLSLTEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYSMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARSWLLKYPEHAITGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDNALLHLGEMLRFPQEEALYPGLLQVKDACTADSLAEFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDASGSRLKDLPKPNKSDDESRADEAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPMVRHLTRRLIWGVYSADNQLQACFRVAEDNSYST
                     ADDDLFTLPEGDISLGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELIRWAGRKCPSGRVMGLANKGWIKGTPQDAGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAERYGWGSNSTQEAQFSVIDAITASELINDIEAL
                     FE"
     gene            7797..11429
                     /locus_tag="QBF46_004016"
     CDS             7797..11429
                     /locus_tag="QBF46_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDQ6676132.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALTEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQAQSVLKSYLSAPPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATEPARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLFVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVAHVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11490..11843
                     /locus_tag="QBF46_004017"
     CDS             11490..11843
                     /locus_tag="QBF46_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676133.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRKLQNIFNQ
                     LIEYYFIKGGSGFPP"
BASE COUNT         3113 a         3052 c         2923 g         2825 t
ORIGIN      
        1 ctttctaaat gcaattaaaa tcaggttgcc aaattaatcg tagcgattta acacgattcg
       61 acgtttgcta tcgctacctg caacaaccac ttctgtcgta acacctttga cgggcgaaga
      121 aacctgatga cgggtgtcaa cattatacgt taactgacca caatgctgtt gtggttgact
      181 attaaccata gccatactgc atgttggggg actgatcctg ccattaatcg ttaatgtggc
      241 agtctctgaa gagaatgcgg gagaagccaa tccataggca agaaaaataa ttcctgacag
      301 ccaatactta ttcattgaac gttatccctg tagtaaaggt tatgcctgga tagaatgagt
      361 gcataacaaa ctatagctgt acatccacta cacagccacg aaggatgata atgaagcatt
      421 gcctgtacga tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa tattcgcagt
      481 attaaataag tgttcattaa tgcggcaaaa acaattacac cgcatcaatg taacatctct
      541 gcggtgtatg aagaataacc agaatggtta ataggcgtta gaccgcgcgg aaggaaatct
      601 cgcctggaat gacttcaccc tgccagtaga gctgagctgc aacgcggtca gccagctggc
      661 ggtagatagc ggtaaattcg ctctccggac ggctgataac cgttggcgtg cctttatcga
      721 gatcctcacg taaggagatg tgtaacggca tctgccccag cagctgtgta tggtattttt
      781 cagccagttt ctctgcacca ccggtaccga aaatcggttc atggtgacca cagttactgc
      841 aaatatgcac actcatgttc tcgacaatac cgagcaccgg aacctcgact ttctcgaaca
      901 tcacaatgcc ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg accacgactg
      961 caccagttac tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg cctggcggca
     1021 tatcaagcac cagatagtcg agatccggcc acaaggtttc ctgcaacatc tgcattaacg
     1081 ccttgctcgc catcggtcca cgccatacca tcgcattgtc gtcagtgacc agataaccaa
     1141 ttgaattggt tgccaggcca tgagacataa tcggtgccat atgggtaccg tccggcgaag
     1201 ttggacgctg attttctgcg cccagcatgg tggggattga tgggccgtag atatcggcgt
     1261 ccagaatgcc gactttcgcc ccttccgccg ccagtgccag cgccaggttt accgccgtgg
     1321 aggatttacc cacgccgcct ttaccggaac tgaccgcaat gatgttcttc acgccgttaa
     1381 tacccggctg attcttcaca cgttttaagg tggcgatatt gtgcgacagt ttccagtcga
     1441 tagctttcgc cccggtgata cgcagcagtt cggcgctaca ttgctctttt aactcttcga
     1501 acgcgctatg ccagacgaat ggcataacca gttccacatg caacgtgtcg tccatccagg
     1561 cgacatggtg caacgctttc agcgtggtga ggttatgctt cagggttggg tgctgaaaat
     1621 tggccagtgt cccggcgacc attgcgcgca aggcttctgg tgatttggcc tgggattgtt
     1681 cgttcatccc gactcctttt ttgtataaat aaaccatcag ctgatagttt acctgaagaa
     1741 tatagagaag tacttactta acattttccc atttggtact atctaacccc ttttcactat
     1801 taagaagtaa tgcctactat gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg
     1861 ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca ggctgatgtc
     1921 tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc
     1981 cacggtacac cgatcatgct gaaagctcag cagcttggta tcaccccgga gcagatgatt
     2041 ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag ctatgacaac
     2101 tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta ctctcgcctg
     2161 aaagaaaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc
     2221 atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa
     2281 tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaact gatcgagccg
     2341 aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt cttctttgat
     2401 ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt gcaggagcag
     2461 gtggcaaata aaatgcagga gtggtttgaa tctggcctgc aacagtggga tatctcccgc
     2521 gacgcccctt acttcggttt tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg
     2581 ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa gcgcggcgac
     2641 agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta ccacttcatc
     2701 ggtaaagata ttgtttactt ccacagcctg ttctggccag caatgctgga aggcagcaac
     2761 ttccgcaagc cgaccaacct gtttgttcat ggctatgtga cggtgaacgg cgcgaagatg
     2821 tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt tgacgctgac
     2881 agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac
     2941 ctggaggatt tcgttcagcg cgtgaatgcc gatatcgtta acaaagtggt taacctggcc
     3001 tcccgtaacg cgggctttat caacaaacgt tttgacggcg tgctggcaaa cgaactggct
     3061 gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga agcgtgggaa
     3121 agccgtgaat ttggtaaagc tgtgcgcgaa atcatggcgc tggctgatct ggctaaccgc
     3181 tatgtcgatg aacaggctcc gtgggtagtc gcaaaacagg aaggtcgcga tgctgacctg
     3241 caggcgattt gctcaatggg catcaacctg ttccgcgtgc tgatgactta cctgaagccg
     3301 gtactgccga aactgaccga gcgtgcagaa gccttcctca atacggaact gacctgggat
     3361 ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaggcgct gtataaccgc
     3421 atcgatatga aacaggttga agctctggtg gaagcctcta aagaagaagt gaaagcagct
     3481 gccgcgccag ttactggccc actggcagat gatccgattc aggaaaccat cacctttgac
     3541 gacttcgcca aagttgacct gcgcgtagcg ctgatcgaaa acgcagagtt tgttgaaggt
     3601 tctgacaaac tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa tgtcttctcc
     3661 ggtattcgtt ctgcctaccc ggatccgcag gcactgattg gtcgtcacac cattatggtg
     3721 gctaacctgg caccacgtaa aatgcgcttt ggaatctccg aaggcatggt gatggctgcc
     3781 ggtccaggcg ggaaagatat cttcctgcta agcccggatg ctggtgctaa accgggtcat
     3841 caggtgaaat aatccccctt caaggcgctg catcgacagc gccttttctt tataaattcc
     3901 taaagttgtt ttcttgcgat tttgtctctc tctaacccgc ataaatactg gtagcatctg
     3961 cattcaactg gataaaatta cagggatgca gaatgagaca ctttatctat caggacgaaa
     4021 aatcacataa attctgggcg gtggagcaac agggaaacga gttgcatatc agttggggaa
     4081 aagttggcac caaagggcaa agtcagataa aaagtttttc agatgctgcg gcagcggaaa
     4141 aagcggaact taagctgatt gcggagaagg tgaagaaggg gtatgtggag caagcgaagg
     4201 ataattcttt gcaaccttcc caaacggtaa cgggctctct caaggtagcg gacttatcca
     4261 ccattattca ggaacaaccc tctttcgtag cagaaacccg tgcgcctgac aaaaatacag
     4321 atgctgtttt accgtggctg gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac
     4381 acaccacgtt aagtcatcgc cgctttcccg gagttcctgt tcagcaagca gacaaattga
     4441 cccaattacg tcgcttagcc tgtagtgtgt cgcaacggga taataaaaca gccacatttg
     4501 acttcagcgc ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc agtcagatcg
     4561 acggcctgaa aacaacacag ttaccatcac cagtaatggc tgtactcacg gcacttgaaa
     4621 tgaaatgcac aagatataaa gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg
     4681 gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt gaatgggatt
     4741 atgcgaataa tgtcattatt attctgccgt ctggcattgc acctagctac ttggagcaat
     4801 attccagatt tgaattacgc ctacgtaaac atttatcact gacggaagag tctctctggc
     4861 aaaaatgtgc acaaaaactt attgccgcaa ttccacatat tccagaatgg cggcaaccat
     4921 taattgcttt gttattaccc gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac
     4981 tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact gatgagcaca
     5041 ttcttgcctc attagaaaaa tatcacgaac catatgccat ttttgatgat tactattgtg
     5101 gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg ccccgatttg
     5161 ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg
     5221 cattgacact gcttatacgt gtagccgggc aaactaaacg ctgtcacgat cggatgacga
     5281 aagccattgc tgcgttccca catgcagcaa tggcggcact gacggaactt cttgggcaaa
     5341 aagaagagaa cagttggcgc attatgctaa tgacaatgct tatctcacaa ccagcactgg
     5401 cagaacaggt cattccctgg ctctcgacac ccgcagttgc cgtactgaaa tcatgccagc
     5461 aacaactgac acagccctca aaccatgcca gcgccgatct actgccagcc gtagtagtct
     5521 cccctccctg gctttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat
     5581 taggcattga gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat
     5641 atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg
     5701 caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag
     5761 ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt
     5821 cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg gagaaagcag
     5881 taaaagcatg gaatgccctt accctatctc cacataccga taccgaatac tccatgttac
     5941 attttggact caaagggtta cctgggttag taaactcact tgcacgctat ccacaagaag
     6001 ccttgcccat cacgaattac ttcgcagcga gtgagctggc acctgccgtc gcccgcgcct
     6061 tcaacaaact caaaacccta cgacaagacg cccgtagctg gctgctaaaa tacccggaac
     6121 atgccataac cggcctgcta cctgcggcgc tcggcaaagc cggtgaagcc caggataacg
     6181 cccgcgctgc cttgcgtatg cttaccgaaa acggtcatca gccattactg caagaaatcg
     6241 cccggcgtta taaccagccg gaagtaacag acgcggtgaa cgctctgctt gcgctcgatc
     6301 ccttagataa tcacccgaca aaaatcccca ctcttccggc cttttatcag ccatcgctct
     6361 ggacgcgccc ggtattaaaa gcaaacgccc aatcactgcc agataacgcc ctcctccacc
     6421 tcggtgaaat gctccgcttc cctcaggaag aggctctata tccgggatta ttgcaggtga
     6481 aagatgcctg taccgccgac tcactggctg aatttgcctg ggatctgttt accgcctggc
     6541 agaccgctgg cgcgccgtcg aaagaaagct gggcgttcac tgcgttaggc gttctcggta
     6601 acgatgacac cgcccgcaaa ctgacgccat taatccgcgc ctggcctggc gaatctcagc
     6661 ataaacgtgc caccgttggg ttggatattc tcgctgctat cggtagtgat atcgccctga
     6721 tgcagcttaa cggcatcgcc cagaaactga aattcaaagc attacaggag cgggcaaaag
     6781 aaaaaattgc cgacattgcc gaaagtcgcg aactcacggt ggcggagctt gaagatcggt
     6841 tagcaccgga tctcggtctg gatgataacg gttcgctgct gctggatttc ggcccacgtc
     6901 agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgcgatgct tccggcagcc
     6961 gcctgaaaga cctgcccaaa ccaaacaaaa gcgatgatga atcgcgggct gatgaggcgg
     7021 ttaaccgcta caaactgctg aaaaaagatg cgcgtaccgt cgccgcccag caggtggcaa
     7081 ggcttgaatc cgccatgtgc ctgcgccgcc gctggtcacc agaaaacttc cagctcttcc
     7141 tggttgagca cccgatggtt cgccacttaa cccgacgtct gatttggggc gtttatagcg
     7201 ccgacaacca gctacaggcg tgctttaggg tagcggaaga taacagctac agcaccgctg
     7261 acgatgatct tttcaccctg ccggaaggcg atatctctct tggtattcct cacgttctgg
     7321 aaatatcacc gacggatgct gccgcctttg gtcagctttt tgccgactac gaactgctac
     7381 caccgttccg tcagcttgac cgtaacagtt acgccctgac agaagccgag cgcaatgcca
     7441 gcgaattgat ccgctgggca ggcagaaaat gcccgagtgg tcgggtaatg gggctggcga
     7501 ataaaggctg gataaagggc accccgcagg atgcaggctg gatcggctgg atgatcaaac
     7561 ctctcggtcg ctggtcgtta atcatggaaa tcgatgaagg ctttgcggta ggcatgtcgc
     7621 cagccgaact cagcgctgag cagctcttaa gcaagctgtg gctatgggaa ggcaaagcag
     7681 aaagatatgg ctgggggagt aattcaacac aggaagcgca gttctccgta atcgatgcca
     7741 tcaccgccag cgagctaatt aacgatattg aagccctgtt tgaataagga aagagcatgg
     7801 acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat aaaaaaggca
     7861 aaacgccgct cagtcatcgt cgctggccgg gcgaaccagt gtccgttatc actggaagtc
     7921 tcatccagac attgggtgat gaattgctac aaaaagctga gaagaaaaaa aacattgtct
     7981 ggcgttatga gaatttttca ctggagtggc agtccgccat cacgcaggcc atcaacttga
     8041 tcggcgaaca caaaccctca atcccggccc ggacaatggc ggcgctagcc tgtatcgcgc
     8101 aaaatgacag ccaacagttg ctcgacgaaa tcgtccaaca agaggggctg gaatatgcga
     8161 ctgaggtggt gattgcacgc cagtttattg cgcggtgtta tgagagtgat cctctggtag
     8221 tgacattgca gtatcaggac gaggattatg gctatggtta tcgctcagaa acctataacg
     8281 aattcgatct ccgactgcgt aagcatctct ctctggcaga ggaaagctgc tggcagcgtt
     8341 gcgccgacaa actcattgcc gcactaccag gaataaccaa agttcgccgc ccttttattg
     8401 cgctgatcct cccggaaaaa ccagaaatag ccaatgagtt ggtaggcctt gaatgcccgc
     8461 gaactcattt tcattctaag gagtggttaa aagttgttgc taatgacccc acagcggtga
     8521 gaaaactcga acactactgg agccaggata tatttagcga tcgagaagcc agctacatgt
     8581 cgcatgaaaa ccacttcggc tacgcggcct gcgccgccct tttgcgcgaa caaggactgg
     8641 cagccattcc gcgcctcgcg atgtatgccc ataaagaaga ttgcggcagt ctgctggtac
     8701 aaattaacca tccgcaagtc atccgcacct tgctactggt ggctgataaa aacaaaccca
     8761 gcctgcaacg tgtagctaaa taccataaaa acttccccca tgcgacgctc gccgcactgg
     8821 cagaactgct ggcgttaaca gaaccaccag cccgccctgg ttatccaatc atcgaagaca
     8881 aaaagctgcc tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc
     8941 tgatggcatc gcagccacaa ctggcagcag aagtgatgcc gtggttaagt actcaagccc
     9001 agtcagtgct gaagagttat ttatcggcac cgcccaaacc ggttattgat ggcaccgata
     9061 acagcaatct gccagaaatc ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag
     9121 ctccacgtct tgatttggca ccgctcgaat taactccgca agtttactgg caaccaggcg
     9181 aacaagagag gcttgccgcc actgagcctg cccgttattt cagcacggaa tctcttgcgc
     9241 aacgcatgga acaaaaaagt ggacgagttg tattacagga actgggtttt ggggatgatg
     9301 tatggctgtt tctgaattat atactccccg gaaaactgga tgctgcacgc aattcactct
     9361 ttgttcagtg gcattactac caggggcggg ttgaagagat cctgaatggc tggaactccc
     9421 cggaagcaca attagcagaa caggcgctcc gcagcggtca catagaagcg ttaattaaca
     9481 tatgggaaaa tgacaactac tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt
     9541 tattggcaca gttgccgcgt gagatggctt tgaccttctg gctgcgtatc aatgagaaaa
     9601 agcatctgtt cgcgggtgag gactattttc tcagtatcct cggattggat gcgctaccag
     9661 gtctgctgtt ggctttttca catcgtccaa aagaaacatt tccgttaatt ttaaattttg
     9721 gcgcaacaga actggcgctg cctgttgccc acgtctggcg acgtttcgcg gcgcagcgtg
     9781 atctggctcg ccagtggatt ttacaatggc cggaacatac ggctagtgca cttatccctc
     9841 ttgtctttac caaacccagc gataatagcg aagccgcatt acttgccctg cgtttactgt
     9901 acgaacaggg acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag cgtacagatg
     9961 tatggtctgc cctggagcag ttgcttaaac agggtccaat ggacatttac ccggcacgca
    10021 ttccaaaagc ccctgatttc tggcatccgg caatgtggtc caggcctcga cttatcacta
    10081 ataatcagcc tgttaccggt gacgctctgg aaattatcgg cgaaatgctg cgctttaccc
    10141 aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc
    10201 tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag
    10261 acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca cgggatctga
    10321 cgacacagat cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga
    10381 acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat atatcgcaac
    10441 gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggtg gtcgcagaaa
    10501 atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg ggccttgatg
    10561 atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa
    10621 acctcaaccc ggttatcttt gatcagcaaa acgttcgcca gaaaagcgtt ccccgtttgc
    10681 gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa
    10741 aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaaacggcc ctacgtacca
    10801 cccgacgctg gtcgctggca gattttcatt ctctgtttgt taatcatccc tttacccgtc
    10861 tggttaccca gcgattaata tggggggttt atccggcaaa tgaaccgcgt tgtttactca
    10921 aagcctttcg tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattgacc
    10981 tgcctgcgga cgctctgatt ggcattgccc acccgttaga aatgacagca gaaatgcgca
    11041 gtgaatttgc acagcttttt gccgattacg aaattatgcc gccttttcgc cagttgtcgc
    11101 gccgcacggt gctgctcaca cctgacgagt caaccagtaa cagcctgact cgctgggaag
    11161 gtaaatccgc taccgttggg caacttatgg gaatgcgata caaaggctgg gagtcaggct
    11221 atgaggacgc atttgtctat gacctgggtg agtaccggct ggtccttaag ttttcacccg
    11281 gttttaacca ctacaatgtt gatagcaaag cgctaatgag cttccgttct cttcgagtgt
    11341 accgtgacaa taaatccgtc acttttgccg aacttgatgt gtttgatttg agtgaggcgt
    11401 taagcgcacc tgacgtcatt ttccattaaa acacagcggg cagtgatgca actgcccgtt
    11461 atcaacacga cttaccagcg gggaaagcga tgatcgtgca aaaagagctg gttgctattt
    11521 acgattatga ggtccctgta cctgaaaatc cgttttcctt cagacttgag atccataaat
    11581 gctctgaatt atttacaggt tccgtctatc gactggagcg attccggcta cgtccaacat
    11641 ttcatcaacg tgatcgagaa gatgctgacc cgctaataaa tgatgcgttg atttatataa
    11701 gagatgagtg tattgatgag cgaaaattac gaggtgaatc acctgaaact gtaatagcaa
    11761 tttttaatcg taaactacag aatatattca accaactaat agaatattac tttattaaag
    11821 ggggaagcgg ttttccccct tagatgcgtt tcaatttgcc cgttacttca acaaatcaat
    11881 caaccgtaac agagccacta atagctccag tac
//
LOCUS       DAOFQD010000143        11905 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.143, whole genome shotgun sequence.
ACCESSION   DAOFQD010000143 DAOFQD010000000
VERSION     DAOFQD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.143"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..178
                     /locus_tag="QBF46_004018"
     CDS             <1..178
                     /locus_tag="QBF46_004018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676134.1"
                     /translation="NKSELAWNENKKWKKNQESLLLNGVEIPIVSLDYEIEINQEIGN
                     YNAFKDTCYLENYR"
     gene            737..1867
                     /locus_tag="QBF46_004019"
     CDS             737..1867
                     /locus_tag="QBF46_004019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676135.1"
                     /translation="MARTYVKQTVQNNEQLFDSLVINAIDFLESSIDDLNIRPKNSIV
                     DFYTAIELFLKARLMLEHWTLILDDPSKANKQKFSVGDFNSVYFNDAVQRLKTIIGIK
                     LDDNILDEFRTLGAHRNQIVHFAHTGYSSTQANKAGVVAQQWSSWHHLYNLLTVEWKD
                     EFIKFKEEFERVHKRMLQQKEFLSTRFNELSKEIDVLRLKKIKIVKCRQCGLEAGKVN
                     DTHKWGDDYECLVCESQGVAVEQVTDTLPCPNCKVPFKFFSSDINKCPQCAYEITTNI
                     LIKLCRDVYVTGDDWWEEGGECVAGCHVCQHAPNSVFYIEGQWSCVSCFDRGWSAVNC
                     PHCDEYVTGDMETIKYFACYKCEDEQRKYILAEAGISESTFK"
     gene            complement(2275..2358)
                     /locus_tag="QBF46_004020"
     CDS             complement(2275..2358)
                     /locus_tag="QBF46_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDQ6676136.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            3098..3451
                     /gene="arsR"
                     /locus_tag="QBF46_004021"
     CDS             3098..3451
                     /gene="arsR"
                     /locus_tag="QBF46_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDQ6676137.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            3505..4794
                     /gene="arsB"
                     /locus_tag="QBF46_004022"
     CDS             3505..4794
                     /gene="arsB"
                     /locus_tag="QBF46_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDQ6676138.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            4807..5232
                     /gene="arsC"
                     /locus_tag="QBF46_004023"
     CDS             4807..5232
                     /gene="arsC"
                     /locus_tag="QBF46_004023"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDQ6676139.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            5861..7084
                     /locus_tag="QBF46_004024"
     CDS             5861..7084
                     /locus_tag="QBF46_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676140.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLAQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFLHKEHPTPKLYKDGQYLDKQIEG
                     LFSTLLTINDNLLRAKAEIASTIIKFLEARITNLSYNDILKYQQEFQKQCYKQVKAFT
                     TLSRYNKIQTWEEMSEYQFEVFQYETLNPKKMSHTPYLKRPLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            7332..7898
                     /gene="slp"
                     /locus_tag="QBF46_004025"
     CDS             7332..7898
                     /gene="slp"
                     /locus_tag="QBF46_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDQ6676141.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            8054..8584
                     /locus_tag="QBF46_004026"
     CDS             8054..8584
                     /locus_tag="QBF46_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6676142.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(8626..9273)
                     /locus_tag="QBF46_004027"
     CDS             complement(8626..9273)
                     /locus_tag="QBF46_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDQ6676143.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(9337..9663)
                     /gene="hdeB"
                     /locus_tag="QBF46_004028"
     CDS             complement(9337..9663)
                     /gene="hdeB"
                     /locus_tag="QBF46_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDQ6676144.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(9779..10111)
                     /gene="hdeA"
                     /locus_tag="QBF46_004029"
     CDS             complement(9779..10111)
                     /gene="hdeA"
                     /locus_tag="QBF46_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDQ6676145.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10366..10938
                     /gene="hdeD"
                     /locus_tag="QBF46_004030"
     CDS             10366..10938
                     /gene="hdeD"
                     /locus_tag="QBF46_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDQ6676146.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            11737..>11905
                     /gene="gadE"
                     /locus_tag="QBF46_004031"
     CDS             11737..>11905
                     /gene="gadE"
                     /locus_tag="QBF46_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDQ6676147.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEE"
BASE COUNT         3501 a         2468 c         2488 g         3448 t
ORIGIN      
        1 gaacaaaagt gaattagcgt ggaatgaaaa caaaaaatgg aagaaaaatc aagaatctct
       61 tttattaaat ggtgttgaaa tacctattgt cagcttagat tatgaaattg aaataaacca
      121 agaaattggc aattataatg catttaaaga tacttgctat ctggaaaatt atagatagcg
      181 ttcacctcaa aggatatgac tgtaaggata tatctgtaat ccttactcag ctgagatagt
      241 cgccatcatg ctaacaggag atttctgaaa atatatctga agtttcaaat ggtcatatac
      301 agattattgt aaaagaatat cacataatat cctgctgatc ctgacctttt accgataatc
      361 gagttgatgc agaggacagc agttctggta ggcgagtaga cacgtactac ggtcaagtca
      421 ttctgtaaca gcgctttctg ctgaccaaat tggctatgca ctattctggc aatcaaccac
      481 tcaagcagct actattgatg attactcagc caatcttatg ttgaaatgca tttatacaag
      541 aggcggagta cagcatttgt gggtcatcaa ccatttcaac gcaagtaaca gagcaaaaag
      601 taaatgagga tggagaagaa aggttgtgta agcctgatca gataattcgg ctcaaaaatg
      661 tcgtgggttg tgacaaaata ttgtctctaa aggtataatg ttcaaaattc gtagggcaat
      721 ataatcaagg ggcattatgg cacgcacata cgttaagcaa acagtacaga acaatgaaca
      781 actttttgat tctttagtga taaatgccat agatttcctt gagtcatcaa ttgatgattt
      841 aaatattagg cccaagaatt cgattgttga tttctataca gccattgaac ttttcttaaa
      901 agcaaggtta atgctcgaac actggacttt aatattagat gaccctagca aagctaataa
      961 acaaaaattt agcgttggtg actttaattc tgtgtatttt aatgacgcag ttcaacgtct
     1021 caaaaccatt attggcatta aacttgatga caatatcctt gatgagttca gaacgttagg
     1081 tgcgcatagg aaccaaattg ttcactttgc acacactgga tattcaagca ctcaagctaa
     1141 taaagcaggg gttgtagctc aacaatggtc ttcatggcac catttatata atttactcac
     1201 tgttgaatgg aaagatgaat ttattaaatt taaggaagag tttgagcgtg tgcataagag
     1261 aatgctgcaa caaaaagagt tcctcagcac ccgattcaat gagctttcaa aggagattga
     1321 cgtcttaaga ttaaaaaaaa ttaaaatagt taagtgccgt caatgtggcc tggaagccgg
     1381 taaagttaat gatactcata aatggggaga tgattatgaa tgtcttgttt gcgaatcgca
     1441 gggcgttgcg gtagagcaag tcaccgatac tttgccttgc ccaaactgta aagtaccatt
     1501 caaatttttc agttctgaca tcaataaatg tccacaatgt gcttatgaga tcaccacaaa
     1561 tatcttaata aaattgtgtc gcgacgtata tgtaacgggt gatgattggt gggaggaagg
     1621 tggcgaatgt gtagctggtt gtcacgtatg tcagcacgct ccaaattcag ttttctatat
     1681 tgaaggtcag tggagttgtg tttcatgctt tgatagagga tggtctgcag ttaactgtcc
     1741 acattgtgat gagtatgtga ctggtgatat ggaaacaatc aaatatttcg catgttataa
     1801 atgcgaagat gagcaacgga aatatattct agctgaagct ggaatttcag agtcaacttt
     1861 caagtaagtt tgctatttct cgcccttaga actatcagga atctacggct tgatacatag
     1921 gcataaggta cgttcacctc tggaaggcgg ccatgttgcc cttttgaaga acccaccgat
     1981 ggggaaggaa ctcggtgaag tggactgact tcacgccggg gaacgctcct ccccatagac
     2041 agactttatc gatcttctaa gagaaaggga ttctggaaaa catcagtggt ttgtcactca
     2101 aaatctcttt tctctgaaaa atctggaaaa attgttaaca ttagcctaac ccaacactgg
     2161 catcgccttc cagaatttct acgctgtaac agtgtggtag tacagggtga caatgcgtta
     2221 aatgttaaag ggctaagagt agtgtgctct tagcccttaa ttacgtttcc gctatcagtt
     2281 cagaagctga agcagaaagc ggatcagttc cagcagcgca attaacgccc ctagaacgat
     2341 gattgcttta tcaatcaccc gttttctcca tgcgatggag tgagaatgca tccgcttact
     2401 catccactgc ctgtcacggc gcatgtctca ttgttagata agaactctct cactccggcc
     2461 agagcatcag ttaacggcac cacccgtact tctgaccagg actttgaaag cgtttatgcg
     2521 cattgccaga gtgaaaatgc ctcagagcta actggataat catacagtac atgcaggtta
     2581 taaaaccagc acgtccttgc aatagtttca gtatggtatt agcattgatg cgttagatga
     2641 tggctatctc actccagtca gagccaccaa ctcagggctg gaaagtaaaa aaccgacgca
     2701 aagtcggttt ttttacatcc ggattcggac aaggcttaat atgacgatga cccagtgaaa
     2761 gtatataaat cgtcactgcg atatataccg aagtgctccc tccgccagct gaagaaatcg
     2821 ctaattcttg caatgttagc cactggctaa tagtattgag ctgttagata agaactctct
     2881 cactccagcc agagccacca actcagggct ggaaagtaaa aaaccgacgc aaagtcggtt
     2941 tttttacgtc ctgattcaga cctcctttca aatgaatagc caactcaaaa ttcacaccta
     3001 ttaccttcct ctgcacttac acattcgtta agtcatatat gtttttgact tatccgcttc
     3061 gaagagagac actacctgca acaatcagga gcgcaatatg tcatttctgt tacccatcca
     3121 attgttcaaa attcttgctg atgaaacccg tctgggcatc gttttactgc tcagcgaact
     3181 gggagagtta tgcgtctgcg atctctgcac tgctctcgac cagtcgcagc ccaagatctc
     3241 ccgccacctg gcattgctgc gtgaaagcgg gctattgctg gaccgcaagc aaggtaagtg
     3301 ggttcattac cgcttatcac cgcatattcc agcatgggcg gcgaaaatta ttgatgaggc
     3361 ctggcgatgt gaacaggaaa aggttcaggc gattgtccgc aacctggctc gacaaaactg
     3421 ttccggggac agtaagaaca tttgcagtta aaaatttagc taaacacata tgaattttca
     3481 gatgtgtttt atccaggagg cattatgtta ctggcaggcg ctatctttgt cctgaccatc
     3541 gtattggtta tctggcagcc gaaaggttta ggcatcggct ggagtgcaac gctcggcgca
     3601 gtactggcgt tagttacggg cgtggtccat ccgggtgata ttccggtggt gtggaatatc
     3661 gtctggaacg cgacggctgc gtttatcgcc gtcattatca tcagcctgct gctggatgag
     3721 tccggctttt ttgaatgggc ggcgctgcac gtctcacgct ggggtaatgg tcgtggtcgc
     3781 ttgctgttta cctggattgt cctgctcggt gctgccgttg ccgccctgtt tgccaatgat
     3841 ggcgcggcgc ttattttgac accgattgtc atcgccatgc tgctggcttt agggttcagt
     3901 aaaggcacta cgctggcgtt cgtgatggcg gccggattca ttgccgatac cgccagcctg
     3961 ccgcttattg tctccaacct ggtgaatatc gtttccgctg atttctttgg cctcggcttt
     4021 cgcgaatacg cctcggtgat ggtgccggtg gatatcgccg cgattgttgc cacgctggtg
     4081 atgttacatc tctattttcg caaagatatt ccgcagaact acgatatggc gctgctgaaa
     4141 tctcccgcag aagcgatcaa agatcctgct acgttcaaaa ctggctgggt tgttttactg
     4201 cttctgctgg tgggattttt cgtcctggaa ccgctcggca ttccggtgag cgccattgca
     4261 gctgtgggcg cgctgatatt atttgtcgtc gctaaacgcg gtcatgcgat taatacgggt
     4321 aaagtcctgc gcggtgcccc ctggcagatt gtcatcttct cgctcggcat gtatctggtg
     4381 gtttatggcc tgcgcaatgc cggattaacg gaatatcttt ctggcgtact caacgtgctg
     4441 gcggataacg gcctgtgggc cgcgacgctc ggcaccggat tcctcaccgc cttcctctct
     4501 tctattatga acaatatgcc gacggtactg gttggcgcgt tgtccattga tggcagcacg
     4561 gcatctggcg ttatcaaaga agcgatggtt tatgccaatg tgattggctg cgatttggga
     4621 ccgaaaatta ccccaattgg tagcctggct acgctactct ggctgcacgt actttcgcag
     4681 aagaatatga ctatcagctg gggatattac ttccgtacag ggattatcat gaccctgcct
     4741 gtgctgtttg tgacgctggc tgcgctggcg ctacgtctct ctttcacttt gtaatgagat
     4801 actgatatga gcaacattac catttatcac aacccggcct gcggcacgtc gcgtaatacg
     4861 ctggagatga tccgcaacag cggcacagaa ccgaccatta tccattatct ggaaactccg
     4921 ccaacgcgcg atgaactggt caaactcatt gccgatatgg ggatttccgt acgcgcgctg
     4981 ctgcgtaaaa acgtcgaacc gtatgaggag ctgggccttg cggaagataa atttactgac
     5041 gatcggttaa tcgactttat gcttcagcac ccgattctga ttaatcgccc gattgtggtg
     5101 acgccgctgg gaactcgcct gtgccgccct tcagaagtgg tgctggaaat tctgccagat
     5161 gcgcaaaaag gcgcattctc caaggaagat ggcgagaaag tggttgatga agcgggtaag
     5221 cgcctgaaat aaagcggcga tatccccccc acaggttgtt agaaaagtgc gctttattta
     5281 tgtcggatgc gacgctggcg cgtcttatcc gacctacaaa gttatgaaaa ttcgatgaat
     5341 agtatgttat ttgtaggcct gacaggcgta gcgcatcagg cgattttgct atttacacag
     5401 tactccctgc gtgagattcc aattatcgcg tccagcatgg tgtatcagtg agctgcttag
     5461 ttatcagcga tacagcgcag tagtttaaag acgtactgga ttatgattta tcagtggttt
     5521 acacaacaaa ttattaaata attataagat accttaaccc attttattat tacacatcta
     5581 aaacatcaca aaaaaaatca cttcacacac tatcagagaa cataataact ttttaaaatg
     5641 agcgcatttt gatagttgtt aacgatttcc tttacgatac tatcactacc ctttttttac
     5701 acacaaatat taccgggtgg agataaaagg gaaatcaaag gtaattatat caggaagata
     5761 taacgcatta catttattgt gtgtagaaga aaatccattc ctgttctaat tatccaattt
     5821 aaacatctta gcataaaaca aatgatgaat aaggaatttt atgtcaattg actttacccc
     5881 aggtataata aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgc
     5941 taaaacgcca gacacaccga agtggccttg cgataattgg gaagaacaac aacccattaa
     6001 ttccacattc tctggagaag gatatatctc tgatcaatat gatttagcgc aacaccaact
     6061 tcaacaaatt aatgcgtgcc acacgaacac tacatataca aatgcagact actcaaaagt
     6121 tgtggctcaa cttgtaagtc ttattactaa cattgaaacg ataagttcga cacaacttac
     6181 gcaacagaca caatcaatac ttaatcagat taataacatt agatatgaaa aaaacaaaag
     6241 tgctgaatgt cgaatcattg ttgtcgctaa tcctaaaccc gacaaggcaa tcataactaa
     6301 aattagcgta gaagagggaa tacccataac attcagtgtc caaaccatgt tttcagatac
     6361 taattttatt gctgaacaac gagctgactt accaactaat ataaaagata ttcaaagcct
     6421 gtatcaaaaa atgacgaagt tatatattga gcacagtgaa aataaaaaca ggatgaaagt
     6481 ctttgccgga accaacttta ttgatttcaa tatgacagga cagaacctgt ccgggttcgt
     6541 tctaaccttg tcaaggtttt atttcgaaga tctacttaat ataaatttca ctgacgcaaa
     6601 tttaggtgac actatttttt tacataaaga acaccctacc cccaaattat ataaagatgg
     6661 acaatatctt gacaaacaaa tcgaaggttt attttcaaca ttattaacca ttaatgacaa
     6721 ccttttacga gcaaaagcgg aaattgcttc gacaataatt aaatttttag aggccaggat
     6781 aaccaatctt tcatacaatg acatattaaa atatcagcaa gaattccaaa aacaatgtta
     6841 taagcaagtt aaagccttta cgacactctc tcgatacaat aaaattcaaa catgggaaga
     6901 aatgtctgaa tatcaattcg aagtatttca atacgagacg cttaacccca aaaaaatgtc
     6961 acacacgcct tatttaaaga ggccacttcc taatgaaaaa gatataaatt atggggtgga
     7021 aatagaaata ccatctggca aaagaattcg cttatcaaat cattatcaga atataatacc
     7081 ataatattga ttattagcac tttataatta ttgaataaaa atagatttta tgtaatttta
     7141 aaacaaagga ttatattatg gggcgatgga tactcatgcc acgccccaaa atcaatggaa
     7201 ttgaactaaa caaaagggat ataaacatca gacaggttta cgttactatc aggcatatcg
     7261 cctcagaatc agatgaaaac tataaagaaa tatctattat ggttttaata tttgttgata
     7321 aggatagtaa catgaacatg acaaaaggtg cactcatcct cagcctttca tttttgcttg
     7381 ccgcatgtag ttcaattccg caaaatatca aaggcaataa ccaacctgat attcaaaaaa
     7441 gttttgttgc tgttcataac cagccggggt tatatgttgg tcaacaagcg cgctttggtg
     7501 ggaaggttat caacgttatc aatggcaaaa cggatacgtt gttagaaatc gctgtattac
     7561 cgttggatag ctatgcgaag cctgatattg aagccaacta tcagggccga ctgctcgcca
     7621 gacaaagcgg cttccttgat ccagtgaact atcgtaatca ctttgttacc atcctcggca
     7681 ccattcaggg tgaacaacct ggctttatca ataaagtccc gtataacttc ctggaagtga
     7741 atatgcaggg catccaggtg tggcatttga gagaagtggt taataccacc tataacctgt
     7801 gggattacgg ctatggtgca ttctggccgg aaccgggctg gggtgcgcct tactacacca
     7861 atgcggtgag tcaggtaaca cctgagctgg tcaaataaca ccaccgaaag atgcagatgc
     7921 tttctcagca tctgcatcat gcattacatc aaattaatac acagtaagct aactattatt
     7981 agtataagcc ctgtcctgtt aattaccttt ggcaaactga ttataaagtt aatgtccgca
     8041 ccaggagtcg gttatgtttc ttataattac cagggatacg atgttcttca ccgcgatgaa
     8101 aaacattctg agtaaaggta atgtcgttca tatacagaac gaagaagaga tcgacgtaat
     8161 gttgcatcag aatgccttcg tcattattga tacattaatg aataatgtat ttcattctaa
     8221 ttttctcact caaattgaac gattaaaacc tgtccatgtc attattttct ccccctttaa
     8281 tattaaacgc tgcctgggga aagtgccggt gacctttgtt ccgcggacta tcactatcat
     8341 tgattttgtc gcactcatca atggcagtta ctgctctgtg cctgaagcgg ctgtgtcact
     8401 ttcgcgcaag caacatcagg ttctgagctg cattgcgaat caaatgacaa cggaagatat
     8461 tctggagaaa ctgaaaatat cgctaaaaac gttctactgc cataaacaca atatcatgat
     8521 gatcctcaat cttaagcgga tcaatgagct ggtacgccat cagcatattg attatctggt
     8581 gtgaatttca ggcttacggt gagtctggct acgctgccac acagattagc taattgaaac
     8641 gcctttcacc cctgccatac cttttaataa tcgcaacagg tcttctatcg acgttgtggc
     8701 atgaagctgg atatcaatgg cgaccacttc atgatcttca ttttcctgca acgaaactaa
     8761 atcagttttt attttttgct gcttaaacca gtcgagcatt gataccgtat taccgttcac
     8821 taatgtcagc tgtaaatggt aatttttgtt catcaggcgg aaggttaatt gatggaagac
     8881 ttccagcacc aacaaggtca tcactgaacc ataaataccc agttcgtaca taccgctgcc
     8941 aataaccata cctatggcgg cggtcaccca gatatccgct gccgtcgtca gacctacaat
     9001 attttggtta cgaacgagaa tgttacctgc accgataaac ccgacgcccg tcaccacctg
     9061 agcagcaata cggctggggt cgagtccgac gtgatctaaa gacagcacgt cagcaaaacc
     9121 atatttcgaa acaatcataa acagggcgct tcccatgcca attaatacat gtgtgcgtaa
     9181 ccctgctcct ttgccgcgca tttgcctttc catgccaata gcgccacagg caattgccgc
     9241 gagtataaga cgtataataa attctgctgt catataaaaa tgcaaaaagg agcagcaaga
     9301 tggctcaact tgctactcct ttttacttgt acctcattaa ttcggcaagt cattagatgc
     9361 ttgatttttg aaggtataca aatttttctg cgggtttttc ttacagtatt cgatcacttt
     9421 aggaatttga gtgagatcgg tttcatttaa agtaacggta tcgccacctt tatatactgt
     9481 ttcttcatgc agcatccacc atgcaaccgg ggtcattgct tttggattca gatcaataaa
     9541 ttcctggcag gtcatatctt tagcggattc attggctgcc agcgcagatt gtgcgttcac
     9601 cagtgacaaa gccgctacag cgcccataaa aataaacgct ttacggagag atgaaatatt
     9661 cattttgtaa cccattcaat atagagatta tatatgaaca ggaggaatag ctgcttttaa
     9721 agaagatatt ccggcctgtt caatatgcaa ggaagtacga tgtaacgcat tcccggaatt
     9781 acatatcttt cttaattttg tcccattcgc ctttaacttt atctttaaag ttggcttgtt
     9841 tatcctgagt acaagcctga acgatagctg gggttacggt tgcaataccc tgaacatcta
     9901 aaaccgcatc ttctggttta tctttgttgt tcagcgcttc agcaaaacca actgcagttg
     9961 gctggaagga ttcgtccaca gccaggaaat cttcacaggt ccaggagttg accggttttt
    10021 tgttatcagc tgctttttgc gcatccgctg cattgctcac aactggcaga agaagcagac
    10081 caccaagaat aacgcctaat acttttttca tcgtaatatc ctcaactata aagtgaaaga
    10141 gccgtcacga atcaatttcg acactgaggt tataacctgg ttttctgtat atgtcatgtt
    10201 gatggaaaat atcaaaatca gatattttta tttcaataca atgagttaca gatgcatcag
    10261 atactgcaat taggaaattt ttattaaatc gactgcattc ttagacgcgt ttttggcata
    10321 gattgatagc aggggatttt cttcttaatt ttatagggtg gttctatgtt atatatagat
    10381 aaggcaacaa ttttgaagtt tgatctggag atgcttaaaa aacatcgcag agcaatccag
    10441 tttattgccg tgctgctgtt tatcgtcggg ttgctgtgta tcagtttccc gttcgtctct
    10501 ggcgatattt taagcacagt agtgggtgca ttattaatct gctcgggtat tgcgcttatt
    10561 gtcgggttat tcagcaaccg cagtcataat ttctggccgg tattatccgg tttcctcgtc
    10621 gcagtcgcct atttattgat cggctatttc ttcatccgcg caccggagct gggcattttt
    10681 gccattgcgg catttattgc cggtttgttc tgtgttgcag gggttattcg cctgatgagt
    10741 tggtatcgtc agcgttcaat gaaaggcagc tggctacagc tcgttattgg tgtgctggat
    10801 atcgtcattg cctggatatt ccttggtgca acgccgatgg tgtctgtaac gctggtttcc
    10861 acactggtgg gaattgaact gatatttagc gccgccagcc tgttcagctt cgccagtttg
    10921 ttcgttaagc agcaataatt accccggttg tcacccggat catagtcact tgatgtgact
    10981 atgatccgat taatactctc tccgctacgc agtgttgtag atcaattgcg cactatcatt
    11041 gaaataatta cctgctagtg attatttcaa cctactgaat ttcatctaat ttttttcact
    11101 ccatggaaaa ttagccattt caaacattat catggctgat attttccgta gtcaggttta
    11161 atgttttaaa agtgctgtgg gaaagtgaac aaagagttcc gtaagcgttg atgctatggg
    11221 cggttaaata agtaatccgg gttcattttt ttgcaactgg cgttgattac attgcataaa
    11281 tatccgtgtc tccagacgct atataaaaac ctgaagacat gaatgcgtta tttactcagg
    11341 taatttcaat gcgttaaaag aaagctggca atccaattgc cagcttaagt cgaaacaagg
    11401 agactcgata tttaaatcgg attacatttt aactttagta atattcttca gagatcacaa
    11461 actggttatt gataacttat tcttgggcag taatccgcaa acgttaactt tttgtttgct
    11521 atttacaagc tgataacaac caggaatctt acttaggatc aatatatgga gtgcgtgatg
    11581 gataaatctg aagtattgat tagtgttaat agacgtatta gttcacgaag ggtaaagttc
    11641 ttataggcgt ttactatatt gaacaacgat tcggacaagg atgtaaataa tgaaaaggat
    11701 gacatgttcg aaacgataac ggctaaggag caagttatga tttttctcat gacgaaagat
    11761 tcttttcttt tacagggctt ttggcagttg aaagataatc acgaaatgat aaaaatcaat
    11821 tccctgtcag agatcaaaaa agtaggcaat aaacccttca aggttatcat tgatacctat
    11881 cacaatcata tccttgatga agaag
//
LOCUS       DAOFQD010000144        11758 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.144, whole genome shotgun sequence.
ACCESSION   DAOFQD010000144 DAOFQD010000000
VERSION     DAOFQD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.144"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..2870)
                     /locus_tag="QBF46_004032"
     CDS             complement(<1..2870)
                     /locus_tag="QBF46_004032"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDQ6676148.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKAEPTGDSYQSESDLERELIQDL
                     QNQGYEFISVKSQSAMLSNVREQLQSLNGVMFNDSEWRRFTEQYLDNPSDGILDKTRK
                     IHIDYICDFIFDDGRLENIYLIDKKNLMRNKVQIIQQFEQTGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAAN
                     KSATYKPLRIATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSYFK
                     TNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMTVVSELEQRFPDPTSIESEKEKKDFVKLFGEYLRAENILQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQTIRLPADRKIQDYRSAYNDIRDW
                     QRREKEAEKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHNRQNKGKGEMIEEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIEAFFTFA"
     gene            complement(2992..4197)
                     /locus_tag="QBF46_004033"
     CDS             complement(2992..4197)
                     /locus_tag="QBF46_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDQ6676149.1"
                     /translation="MSEMSYLEKLLDGVEVEWKTLGQTCKIETGKLNANAAVDDGEYM
                     FFTTAKETSKIDKFRWDTEALLIAGNANVGEVKHYIGKFEAYQRTYVLTNFDENVSVR
                     FLYFVLSHSLKKYLEERTNSAAMTYIVLSTLENFPIPIPCPDNPEKSLAIQSEIVRIL
                     DKFTALTAELTAELNMRKKQYNYYRDQLLSFKDGEVEWKTLGEIGDFTYGYAAKAMDS
                     GDARFVRITDINKDGKLSKENPMYVELNEENEKYTLDKNDLLMARTGATFGKTMIFEE
                     DYPAVYAGFLIKLNLNKTIINAKYYWHFAQSDFFWEQANKLVSGGGQPQFNANALKQV
                     RVPIPYPSHPQKSLDEQGRIVDILDKFDAIAASITEGLPREIELRQKQYEYYRDLLFS
                     FPKPETASN"
     gene            complement(4194..5750)
                     /locus_tag="QBF46_004034"
     CDS             complement(4194..5750)
                     /locus_tag="QBF46_004034"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDQ6676150.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTNV
                     NKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDNREIIDIGELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            5933..6154
                     /locus_tag="QBF46_004035"
     CDS             5933..6154
                     /locus_tag="QBF46_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDQ6676151.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            6154..6534
                     /locus_tag="QBF46_004036"
     CDS             6154..6534
                     /locus_tag="QBF46_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDQ6676152.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVAATLRRLYGSAE"
     gene            6539..6718
                     /locus_tag="QBF46_004037"
     CDS             6539..6718
                     /locus_tag="QBF46_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HDQ6676153.1"
                     /translation="MARKYNKLSREALKMLLDGVSRRKVKQYLVSKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            6746..7789
                     /locus_tag="QBF46_004038"
     CDS             6746..7789
                     /locus_tag="QBF46_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDQ6676154.1"
                     /translation="MKAIITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDSLVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSDGH
                     DLSIQELCWWAFMCNMMHLMPEEVCRISINKMKAATQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGVITSHSSSVR"
     gene            7878..8330
                     /locus_tag="QBF46_004039"
     CDS             7878..8330
                     /locus_tag="QBF46_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HDQ6676155.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSLLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVVRQHRHYGNATSARI"
     gene            8417..9610
                     /locus_tag="QBF46_004040"
     CDS             8417..9610
                     /locus_tag="QBF46_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDQ6676156.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTGTADSRKPNKPVRKPLKSMIIDHANDQRAGDHIVAETAEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEAWKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEERQTAVGGPSLEDLDKVARERAA
                     NRRADAALWIEQRREEIADIVDTGGYGDVDTEGVSNDPWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            9610..11094
                     /locus_tag="QBF46_004041"
     CDS             9610..11094
                     /locus_tag="QBF46_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDQ6676157.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDLIIES
                     VQEQGSKTSVSSGHGTGKSDMTSIMIMLFIIMYPGARAIIVANKIQQVMTGIFKYIKI
                     NWATATSRFPWLADYFVLTETAFYEVTGKGVWTVVPKGFRLGSEEALAGEHADHLLYI
                     IDEASGVSDRAFGIITGALTGQDNRILLLSQPTRPSGYFYDTHHKLAKRPGNPDGVYT
                     AITLNSEESPLVTPEFIKMKLAEYGGRDNPMYMIKVRGLFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWLACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKATADLMYEYYGITVQRIRWGKKMHSREDKS
                     LYFDKRAYANVQAAEAVKSGRMRLDKGNETIEEASKIPVGINSAGQWKVMSKEDMKKK
                     LNLHSPDHWDTYCFAMLADYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            11091..11655
                     /locus_tag="QBF46_004042"
                     /pseudo
     CDS             11091..11655
                     /locus_tag="QBF46_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817943.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
BASE COUNT         3054 a         2681 c         2801 g         3222 t
ORIGIN      
        1 gcaaacgtaa agaatgcctc aatgatactg gctttgtctg gtaaatcatc caggttcgtt
       61 tgctgaataa aatcgaccac caggccctct ttcgcccggt tccccaggct tgaacgaatt
      121 aagcgtttga cctcttcgat catttcgccc ttgcctttat tttgtctgtt gtgttcgaaa
      181 atcagtccaa ggatataatc caggtttatt tcctgagact tcagcaaatc gacctcaaaa
      241 accacgtcat cccagtcagt ggttgatttc tcttttttct cagcttcttt ctcacggcgc
      301 tgccagtcgc gaatatcgtt ataggcagaa cgataatcct gaatcttgcg atcagcaggg
      361 agacgaattg tttgcaattc agcgaacttt tcatcatcca cataatgttc tgctttgaat
      421 ttttctaccg caacaggatc gctaagatcg atttgttgca gggcttttag cgtggcaaat
      481 tcatcatagt tttgcaggat gttctcggca cgcaggtatt cgccaaacag tttaacgaag
      541 tctttcttct ctttttcact ttcaatactg gtagggtcag ggaaccgttg ttccagttct
      601 gaaactactg tcatgaagcc gcgtttagct tcaccagtgg cagcatcagt aaagccttcc
      661 atatactctg cataactctt ttctaaaacc acatttttgg tatttttgtc accaaacagc
      721 gttatggcat caatggttga gcgttccaga tcccggaacg tgacaatatt accgaaggtt
      781 ttagtagcgt cataaatgcg gttggtgcgg gaaaatgcct gcatcaggcc gtgaaaacgc
      841 aagtttttat cgacgaatag cgtgtttaat gttggagcat cgaagccggt taaaaacatt
      901 cccacgacaa ttaacagatc gatatcctga tttttaaccc gttgggctaa atcacgatag
      961 tagttctgaa aaccgttact gtcggtgctg aagttagttt taaaatagct gttatactca
     1021 cgaattgcag catccagaaa ctctttagca ctgctgtcca ttgcgctggt atcaaaagtt
     1081 tcatcggaaa tttcaccaat ggcattttgt tcttcattgg cggcaaagga gaagattgtc
     1141 gcaatacgca gcggtttata ggtagccgat ttattagcgg cttcttcttg taaccgttta
     1201 aacgtcgcat aataggcttt cgcggcatcc acgctgctca ctgccaacat agcattaaaa
     1261 ccttttgagc cagggaaggt acggtgggtt ttctggcgga aattattcag aatatattgc
     1321 gtgatttcct gaatacgcat gggatgaaga aacgcctgct gattttcagc cgcactcagt
     1381 tttttctcgt cagtttctgt ctctaaagac ttaaactgtg gccgcacatc gttgtagtcc
     1441 accttgaatt tgagcacttt ttcgtcacga atcgcatcgg taattacata cgaatgcaat
     1501 tcacgaccaa atacgctggc ggttgtttct gagcctaagg cgttttccgg gaaaataggg
     1561 gtgccggtaa aaccaaactg ataatagcgt ttgaatttct tcttcaggtt tttctgcgct
     1621 tctccaaact ggctgcggtg gcattcatca aatataaaca ccacttgctg attatataca
     1681 ggcaggtcgc tttctgcttt catcaggtta ttgagtttct gaatagtagt gacgataatt
     1741 ttgttatcgt ccttatccag atttcgttta aggcctgcgg tattttccga gccgttgacg
     1801 ctgtctggcg aaaaacgctg atattccttc atggtctggt aatcgaggtc tttcctgtcg
     1861 accacaaaga agactttatc aataaagtcc agctctgttg ccagacgcgc ggctttaaag
     1921 ctggtgaggg ttttaccaga accggtagtg tgccagatat acccaccgct ttccggtttt
     1981 gaccagttct tcgctgtaaa ggaactctta attttccaca gaatgcgctc ggtggcggca
     2041 atctggtacg gtcgcatcac cagtagcgtc tgactactgt caaaaacgct gtagttcacc
     2101 agaacattca gcagagtatg tttctggaaa aaggtagcgg taaagtcttt gaggtcttta
     2161 atcagcgtgt tgtctgattt cgcccaattc atggtgaagt caaaactgtt tttatcgcgc
     2221 tttgtcgtgt tggcaaaata acgggtatcg gtgccgttag aaatgacaaa cagttgcaga
     2281 tacttaaaca gggaattttc gctgttaaaa ctctctttac tgtaacgatg tatctggttg
     2341 aaagcctcac gaatcgccac cccgcgtttt ttcagttcga tttgcaccag cggtaaacca
     2401 ttaaccagga tcgtgacgtc ataacggtta gcatgagaac ccgtctgttc aaactgctgg
     2461 ataatctgca ccttattgcg catgagattc tttttatcta tcaaatagat gttctcaagt
     2521 cgcccgtcat caaaaataaa gtcgcagata tagtcgatat ggattttacg ggttttatcc
     2581 agaataccat cgctggggtt gtccagatac tgctccgtga agcgccgcca ctcgctgtca
     2641 ttaaacatca caccattgag gctctgaagc tgttcccgaa cattggacag cattgccgac
     2701 tgtgatttta cggaaataaa ttcataaccc tgattttgca ggtcctgaat cagttcacgt
     2761 tccaggtccg attcgctctg gtagctgtcg cctgttggct cagctttgat gtacttatca
     2821 agaacgataa agttattgga ttcagcaatg gtgtgtgtct gatgagtcat agcgcatcct
     2881 ttgtgccgtc tggcaagggc cggaaggggg gtaatggtga cttccggcgc gtaaaaaata
     2941 gtctatatac tgaccgggtg ttaaggtggt ccgatcggta gcaatgatca attaattact
     3001 ggcagtttca ggtttcggga aactgaacag taaatcacgg tagtattcgt attgtttctg
     3061 gcgcaactcg atttcacgcg gaagaccttc ggtgatggaa gctgcgattg catcaaactt
     3121 atccagaata tccacaatac gcccctgttc gtctaaagat ttttgagggt gagatgggta
     3181 tggaataggt actctgactt gtttcagtgc attagcatta aactgtggtt gcccaccacc
     3241 agatactaat ttattagcct gttcccaaaa aaaatcactt tgtgcaaagt gccagtaata
     3301 tttggcatta ataatcgttt tatttaggtt caactttatt aaaaaaccag cgtatactgc
     3361 tggataatct tcttcaaaaa tcattgtttt accaaaggtt gccccagttc ttgccatgag
     3421 taaatcattt ttgtctaagg tatatttctc attttcctca tttaattcaa cgtacattgg
     3481 gttttctttc gatagcttcc cgtctttatt tatgtcagta attctcacga aacgggcatc
     3541 tcctgaatcc atggctttgg ctgcatatcc atatgtaaaa tctccaattt cccccaaagt
     3601 cttccactca acctcaccat ctttaaaact caacaactgg tcgcgatagt agttgtactg
     3661 ttttttacgc atgttaagct cagcggtaag ctcagcggta agtgcagtaa atttatccag
     3721 aatccgaacg atttcagact ggatggcaag ggatttttcc ggattatccg ggcaggggat
     3781 ggggataggg aaattctcta aggttgatag cacgatatat gtcattgccg cgctatttgt
     3841 tctttcctcg agatatttct tcaggctatg gctaagcaca aaatacaaaa atctaacact
     3901 tacattttcg tcgaaatttg tcagaacata ggtgcgctga taagcctcaa acttgccgat
     3961 gtaatgcttc acctcaccaa cattcgcatt gcctgcaatc agcaatgcct ctgtatccca
     4021 gcggaattta tcaattttgc ttgtctcttt ggctgtcgtg aagaacatat actctccatc
     4081 atcaacagcc gcattcgcat taagctttcc tgtttcaatt ttgcaagttt gcccaagtgt
     4141 cttccactca acctcaaccc catccagcaa tttttccaga taactcatct cgctcatttc
     4201 tgcacctcgc agccttcaat ttcagccaca attgcatcaa tatctttacg caactggtcg
     4261 attttgctga ccgtggtttt cagctctgca ttcagctcac cgatatcgat aatttcgcgg
     4321 ttatctttcg cttccacata gctgctcacc gacaggttat agtcattcgc gacaacagtc
     4381 tcaaacgcaa cagatttcgc cagatgagca acatcttcct tgctggcaaa tacctgcata
     4441 atctgttcga tatgggcatc ggtcaggata ttgttgttgg tttctttttt gaacagttcg
     4501 ctggcatcaa taaactgaac tttggtatcc gttttatgtt tagacagcac cagaatgttt
     4561 acagcaatag tggtgccaaa gaacaggttc ggtgccagtg aaatcacggt ttcgacatag
     4621 ttattatcga ccagatactg acggattttc tgctccgcgc cgccacggta aaaaataccc
     4681 gggaagcaga caatcgcagc acgacctttg gccgaaagat agttcagcgc atgtagtaca
     4741 aacgcaaagt cagctttgga tttgggggcc aggacgccag ccggggcaaa acgttcatcg
     4801 ttaatcagcg tcgggtcatc gctgccaatc catttcaccg aatacggcgg gttagaaacg
     4861 atggcatcaa acggtttttc atctctgaag tgtggctcag tcagtgtatt acccagcttg
     4921 atatcaaact tgtcgtagtt gatgttgtgc aaaaacatgt tcatacgtgc caggttatag
     4981 gttgtatggt tgatttcctg accaaaaaag ccttcttcga tgatatggtt atcaaactgc
     5041 tttttagcct gcaacaacag tgagccggaa cccgctgccg ggtcgtagat tttgttaacg
     5101 ttggtctgcc cgtgcatagc cagttgtgca atcagcttgg agacgtgctg cggtgtaaag
     5161 aactcaccgc ctgacttacc ggcatttgcc gcatagttag aaatcaggaa ctcataggca
     5221 tcaccgaaca ggtcaatctg atgttcgttg aagtcaccaa gttttaaccc ttcaacccct
     5281 ttcagaaccg cagccaggcg ggcattttta tctttaacgg tgttacccag gcggttactg
     5341 gtggtatcga aatcagcaaa caaacctttg atgtctgctt ctgaagggta gccgtaagca
     5401 gaactttcga tagcaacgaa gatgctgttt aaatctgcgt tcagtctgtc attggtattt
     5461 gctttcgcag ctacgttgca gaaaagctga ctggggtaga tgaagtagcc tttagttttg
     5521 atggcatcgt ctttaatgtc atcagtaatt acgctgtcat ccagtttcgc ataacagata
     5581 ctgtcatcac cggcttcaat ataactggaa aaattttcgc tgataaaacg gtagaaaagt
     5641 gcgcccagaa cgtattgctt aaaatcccat ccatcgaccg aacccctgac atcgttagca
     5701 atttgccaga tttgacgatg aagctctgca cgttgttgaa tacttgtcat tttcatccac
     5761 ttatttcagg cttatgtaat tggcggtgat tctacagcaa cttggatgct ttagcagttc
     5821 ggacattagg ctacgaatga cctgcctaga ggtttgttaa gccgcaaagt gctggtgctt
     5881 tatgcctgtg aagtttataa ttgtgtacac ataacgagta cacgaggtgt ttatgcaatc
     5941 cattaacttc cgtaccgcgc gcggcaacct ttctgaagtg ctcaacaatg ttgaagccgg
     6001 ggaagaggtt gaaatcaccc gcagaggtcg tgagccagca gtaattgtca gcaaggctac
     6061 tttcgaagcc tacaaaaaag cggcgctgga tgctgaattt gcatccctgt ttgacaccct
     6121 ggactccacc aacaaggaac tggttaaccg ataatgaggc atatatcacc ggaagaactt
     6181 attgcgcttc atgatgcgaa tataagccgc tacggcggcc tgccgggaat gtcagatccg
     6241 ggtagggcag aggccattat cgggagagtt caggccagag ttgcctacga agagatcacc
     6301 gaccttttcg aagtctccgc cacctacctg gtggctacgg cgagagggca tatattcaat
     6361 gatgccaata agcgtaccgc gctaaacagt gcgctgctat ttctacgccg taacggggtg
     6421 caggtatttg attcacctga actggcagac cttactgtag gcgctgcgac tggcgagata
     6481 tctgtatctt ctgtcgccgc cacgttacgt agattgtatg gttctgcgga gtagattaat
     6541 ggcacgcaaa tacaacaaat tgtcccgtga agcgttaaag atgcttcttg atggcgtgag
     6601 tcgccgcaag gtaaagcaat acctggttag taagcaaatt ggtgccagga ccgctattgc
     6661 tgtgttatgc cgtcaggaaa tggttgtgct taaacagaga atgccgggca gcagataaag
     6721 cccaatcagt gatgaaaggt gtgatgtgaa agccattatt actccctttg tacaaaaaga
     6781 gcttggcgtc gccacattca aagtggatca ggaagtcaga aagctggtgg aggctggccg
     6841 taaatttatt atggagccgg tgccgcgtga gttaatcgag cacatggacg acggcctcgt
     6901 tgtttccgag caaaccatgg caacaaatga ggcgttgcag ccgtttttta acagcgatga
     6961 actgtttcgc cgtattggtg gaattgactc gctggtggcg tggttgcgca ggaaagaggg
     7021 gcaatgccag gccgcagatc gtagttggtg tgacaaccat attgtccacg ctgaacgaga
     7081 caatagcgcg gtgttgttgt gctggcatca cgataaccat taccggatgc gtggttttaa
     7141 tgagctgaaa gaaacgctgc acaataatcg cgttaactgg atactggatg tcgcccgtca
     7201 ggaaatgggg ctttcagatg gccatgattt aagtattcag gaactgtgct ggtgggcttt
     7261 catgtgcaac atgatgcacc tgatgccgga agaagtttgc cgtatatcaa taaataagat
     7321 gaaagccgca acgcaggata gcggacctct gaaagaggcg gatattcgcc cgtatgacga
     7381 tcgcgctaca gcatatgttc agatgatgga agaacgcgcc gcgccgatgc gtgcaaaagt
     7441 atgccctgtg gatgttgact ccgaccctgg catggcgcat ttcaaaatac caaaactgca
     7501 atcgctaaaa ttacctgagt acatggactt tgttgcttcc cgtccatgct gtgggtgcgg
     7561 agcggcggga gctggcgctc acattacgcc ttatatcgtt cgtcatagtc gattatgcgc
     7621 gcatgacatt tacgcaattc ctctgtgcca gtcatgccag cgtgatattg agcgtgaccg
     7681 cgataattgg gagaagacgc acggtaggct ggcgatgcat caacgattgt tctttgatta
     7741 cgcgcttgga gtcggcgtta tcacaagtca ttcgtcgagt gttagataaa attgctctaa
     7801 tgtattgcta tttctttagt tgagggtatt atattccacg ttgattagtt gacatgggct
     7861 aatcagtagg tgacaggatg ttacttaact ggcagggacg ccacttcatg gaaataaatc
     7921 actcacgaat aacatcgtac gagattgcgg attacatgat ccgcactaaa tctcttctat
     7981 cagcgaaaga actcgcagca attcttgaaa aggaataccc gcatctggat gtcgataagc
     8041 gcgatgttta tctgcgctta aaggctatcg ctgtgtctaa gtattcgtct gttttgattg
     8101 atgacagtac acgcccacgt agatttcaga tccactctct gaatcctgaa ttctttcgcc
     8161 gcagccgcgc tccgcgccgg tttgatgaaa aactccagaa cgaactctat atgacgcagg
     8221 acgaaaagga acgccgggag caccagcctt gggtaatggc gcgtcaactt ttcaataagg
     8281 tggtccgtca gcaccgtcat tacggtaatg ccacatccgc acgtatctga ttgattgctt
     8341 gcccgttccg ggccttttga catgtgactt tcgttaccct cgcgtcaaaa agagttttat
     8401 acgaaaggaa gcataagtga cctgggacga tcacaagaag aattttgctc gcctggcgcg
     8461 agatggtggt tacaccatcg cacaatatgc cgccgagttt aatctcaacc caaacaccgc
     8521 acgtcgttat ctccgtgcat tcaaagaaga caccggaaca gcggatagcc gtaagccaaa
     8581 taagcctgtc aggaaaccac taaaaagcat gatcattgat cacgctaatg atcaacgtgc
     8641 aggtgatcac attgtggctg aaacggctga aaaacaaaga gttaatgctg ttgtcagtgc
     8701 cgcagtcgag aacgctaagc gccaaaataa gcgcataaat gatcgttctg atgatcatga
     8761 cgtgatcacc cgcgcccacc gtaccttacg tgatcgcctg gaacgcgaca ccctggatga
     8821 tgatggtgaa cgctttgaat tcgaagctgg cgattacctg atagataacg ttgaagcgtg
     8881 gaaggccgcg cgcgctatgt tgcgtcgttc cggagctgat gttctggaaa ccactcttct
     8941 ggaaaagtct ctttctcatc tccttatgct ggagaacgcc cgggatacgt gtattcgcct
     9001 ggtgcaggaa atgcgcgatc agcaaaaaga cgatgatgaa ggtactccgc ctgaataccg
     9061 tatcgcgagc atgctaaaca gctgttccgc gcagataagc agcctgatca acaccattta
     9121 cagcatccgg aataactatc gaaaagaaag ccgggaggcg gaaaagcacg ctttgtctat
     9181 ggggcaagct ggcattgtta agctggcata cgaacgaaag cgtgaaaata actggtcagt
     9241 gctggaagca gctgaattca tcgaggcgca tggcgggaaa gtgccgcccc tgatgctgga
     9301 gcaaatcaaa gccgatctgc gtgctcctaa gaccaatacc gatgatgagg aaaggcaaac
     9361 agccgtcggt ggcccttctc ttgaagatct ggacaaagtt gcgcgagaac gggccgccaa
     9421 ccgccgcgcc gatgccgcat tgtggattga gcagcgtagg gaagaaatcg ccgatatcgt
     9481 tgatacaggc ggttatgggg atgttgatac tgaaggtgta tcaaacgacc catggctgga
     9541 acaagacctg gacgaagacg aggaggaaga cgaagaagtc acccgcaagc tatacgggga
     9601 tgatgattaa tggccagaag ttgcgtaacg gatccacgtt ggcgcgagct ggtggcgcta
     9661 tatcgttatg actggattgc ggccgctgat gttttgttcg gcaaaacacc tacctggcag
     9721 caggatctga ttattgagtc tgtgcaggaa cagggtagca agacatctgt ttcgtctggt
     9781 cacggtaccg ggaaatcaga catgacttct atcatgatca tgttgttcat aatcatgtat
     9841 cccggtgccc gcgccattat cgttgcgaac aaaattcagc aggtaatgac cggtatattc
     9901 aagtacatca agataaactg ggctactgcc accagccgtt ttccatggct tgctgattat
     9961 tttgttctga cagaaaccgc tttctatgag gttactggta aaggtgtatg gactgtagta
    10021 ccgaagggct ttcgtctggg aagtgaagaa gctctcgccg gtgaacacgc agatcatctt
    10081 ctgtatatta tcgatgaagc ctccggtgtc agtgatagag ctttcggtat catcaccggt
    10141 gctcttaccg gacaggataa ccgcatctta ttactgtcac agcctacacg cccaagcggc
    10201 tatttctacg atactcacca taaactggcc aagcgtcctg gtaacccaga tggcgtttat
    10261 acggcgatca cgcttaacag tgaggaatca ccgctggtaa cgccagaatt tatcaaaatg
    10321 aagctggcgg agtacggcgg gcgtgataac cctatgtaca tgattaaggt gcgcggccta
    10381 ttccctaaat cacaggacgg cttccttctt ggacgtgatg aggttgaacg tgcaacgcgg
    10441 cggaaagtta agattgccaa aggatggggc tggcttgcat gtgtggacgt tgctggtggt
    10501 acgggacggg ataagtcagt tatcaatatc atgatggtgt ccggccagcg aaataaacgc
    10561 cgtgtaatca actatcgaat gctggaatac acagacgtta cagaaacgca gcttgccgcc
    10621 aaaattttcg cagaatgtaa tcctgagcga ttccccaata tcaccatagc gatagatgga
    10681 gatggcctgg gtaaagcaac agcggatctg atgtacgagt attatggtat taccgtacag
    10741 cgtatacgct ggggtaaaaa gatgcatagc cgtgaagata agagcctgta ctttgataaa
    10801 cgtgcttatg ccaacgttca agccgcagag gccgtaaaat ctggtcgtat gagactggat
    10861 aagggtaatg aaactattga ggaagcgtcg aaaatccctg tagggattaa ctccgcaggt
    10921 caatggaagg tgatgagtaa ggaggatatg aagaaaaaac tcaacctgca ctcaccagac
    10981 cattgggata catattgttt cgctatgctg gcggattatg ttccccagga tgaagtgctt
    11041 agcgtcgaag acgaagcgca ggttgatgag gctctggcat ggcttaatga atgaatattt
    11101 gctctaataa attgtgtttt ttaactaccg atgttacatt gagcctgacc tcttgcgcct
    11161 tgaggcattt tcggtttatg cttatcaggc acctcattaa aacgggtgcc gggattggcc
    11221 tcccgcttaa gtctaaggcg atacagacgc cgctcgcgtc ttttttttgt atcggcgtac
    11281 acgcacacct ctacaatggt gggctgtatg gggctacctt cgggtaggct ggttaccttg
    11341 gacgccagta aggccaactc cgtacagtcc accgccagca agattggtct cttctgcggt
    11401 ggttacatac caacgtctaa ggaggctgcc aatatggcta ctatccctac cccaactcat
    11461 cctgaattta tctggcgctt ttactcctgc caaaaacgtc actatcactt cgttattgca
    11521 ccaacagaag atgaggcccg ctctcagctt cctgacgccc catgtatttt ctctgcccgt
    11581 ttttccactg attcacgcaa ttctctcagt tactggtgcc tccctgttaa cgcttctgct
    11641 caggagggac tatgagaacg tcattagtca cccgtgaaga gatgatcgag gcaattgaac
    11701 agcacactgc ctgtatcagt accagggata taccaggcgt tattgccaac tacttcat
//
LOCUS       DAOFQD010000145        11593 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.145, whole genome shotgun sequence.
ACCESSION   DAOFQD010000145 DAOFQD010000000
VERSION     DAOFQD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.145"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            50..496
                     /gene="ivy"
                     /locus_tag="QBF46_004043"
     CDS             50..496
                     /gene="ivy"
                     /locus_tag="QBF46_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDQ6676158.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(539..2983)
                     /gene="fadE"
                     /locus_tag="QBF46_004044"
     CDS             complement(539..2983)
                     /gene="fadE"
                     /locus_tag="QBF46_004044"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDQ6676159.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALVKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            3223..3801
                     /gene="lpcA"
                     /locus_tag="QBF46_004045"
     CDS             3223..3801
                     /gene="lpcA"
                     /locus_tag="QBF46_004045"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDQ6676160.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4007..4774
                     /locus_tag="QBF46_004046"
     CDS             4007..4774
                     /locus_tag="QBF46_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDQ6676161.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(4745..5485)
                     /locus_tag="QBF46_004047"
     CDS             complement(4745..5485)
                     /locus_tag="QBF46_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDQ6676162.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            5786..6535
                     /locus_tag="QBF46_004048"
     CDS             5786..6535
                     /locus_tag="QBF46_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDQ6676163.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRISRLAEPFWQDHFLGARRILTEETIL"
     gene            6711..7208
                     /gene="rayT"
                     /locus_tag="QBF46_004049"
     CDS             6711..7208
                     /gene="rayT"
                     /locus_tag="QBF46_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HDQ6676164.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHT
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(7432..9144)
                     /locus_tag="QBF46_004050"
                     /pseudo
     CDS             complement(7432..9144)
                     /locus_tag="QBF46_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            9116..9901
                     /gene="lafU"
                     /locus_tag="QBF46_004051"
     CDS             9116..9901
                     /gene="lafU"
                     /locus_tag="QBF46_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDQ6676165.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQYGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9972..11027
                     /gene="dinB"
                     /locus_tag="QBF46_004052"
     CDS             9972..11027
                     /gene="dinB"
                     /locus_tag="QBF46_004052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDQ6676166.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            11024..11476
                     /locus_tag="QBF46_004053"
     CDS             11024..11476
                     /locus_tag="QBF46_004053"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6676167.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESCWKEKLAKSQVWVAVINAQPVGFISRIERHIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQIVKQQHVECRGAWFTNFYMRYKPQH"
BASE COUNT         2744 a         3095 c         3061 g         2693 t
ORIGIN      
        1 caggctttag gaggttaata acatgggcag gataagctcg ggaggaatga tgtttaaggc
       61 aataacgaca gtcgccgctc tggtcatcgc caccagtgca atggcgcagg atgatttaac
      121 cattagcagc cttgcaaagg gcgaaaccac caaagctgca tttaatcaga tggtacaagg
      181 gcataagctg cctgcctggg tgatgaaagg cggtacttat actcccgcac aaaccgtaac
      241 gttgggagat gagacgtatc aggtgatgag cgcgtgcaaa ccgcatgact gtggctcgca
      301 acgtatcgct gtgatgtggt ccgagaaatc taatcagatg acggggctgt tctcgactat
      361 tgatgagaaa acgtcgcaag agaaactcac ctggctgaat gtgaacgatg cgctttcgat
      421 tgatggtaaa acggtgttgt tcgcggcgtt gaccggcagc ctggaaaacc atccggatgg
      481 ctttaatttt aaataattag cggataaaga aacggagcct ttcggctccg ttattcattt
      541 acgcggcttc aactttccgc actttctccg gcaactttac cggcttcgtc gccagctctt
      601 ccggatcaaa gtcatcaacg ttaatactgc gcagacggct ttcttcagct ttcaccagaa
      661 tagcggcttc atctttatca atcagcccct tcaccagcgc gttgtgcgcc agttcatcca
      721 gacgggtaaa cggcaggttt ttacccagct ctttacagat ccgctgatga attgggtcgg
      781 cggcaatcac atccaccagc gcctcttcca gcaagccaac cggattatgc tcgctcggcg
      841 tcaggtactg accgcgacca atgcgggaac gggtggcgtt cggcacttgt aaaatcttcg
      901 ccactttatg atccagcttg tcagaaggtg ccagataatg acgtccggtc gggaagatca
      961 ccacattcag cagcccggca accacgcggt tcgggaagtt ttgcagtaaa tcatccatcg
     1021 cctgttcagc ctgatacagc gcatcttgta cgccccagtg caccagcggc aggtcggctt
     1081 cattacggcc ttcgtcgtca taacgcttca gcacggcaga ggcgaggtag agctggctta
     1141 aaatatcccc cagacgggcc gagatgcgct cgcgacgttt caggctgccg cccagcactg
     1201 ccatcgagac atcagaaagc agggcgaggt tggcgctcag gcggttcagg tgctgatagt
     1261 agcgtttagt ggcatcgccg gttggcgtgc tgctggttaa accgcgcgtc aggcccagcc
     1321 agaagctgcg aactttgttg ctaccgacgt gaccgatatg tttgaacaac agtttatcga
     1381 acgcgttgac gtcattgttc ttcgccgctt ccatctcttc cagcacgtac ggatggcaac
     1441 gaatcgctcc ttgtccgaag atcatcatgc tgcgggtcag aatgttagcc ccttcaacgg
     1501 tgatggcaat cggtgcgccc tggtaagcac gcgccaggaa gttgctttgc ccgagcataa
     1561 tgcctttacc gccggtaata tccatcgcat caataatcga ctgctgcccg cggtgggtac
     1621 agtgatactt aacgatagcc gacagcacgg caggtttttc gccgagcata atgccgtagg
     1681 taatcagcga tgccgcagca tccatcacgt aggcattacc ggcaatacgc gccagcggct
     1741 cttcaatccc ttccatctta ccaatagaga ttttgaactg acggcgaatg tgagcatacg
     1801 cgccggttgc cagcgctacc gatttcacgc cgccggttga gttggaaggc agggtgatgc
     1861 cgcggcctac cgagaggcac tccaccagca tccgccagcc ttgcccggcc attttcggcc
     1921 cgccgatgat gtaatcgatc ggcacgaaga catctttacc gcgcgtcggt ccgttctgga
     1981 acggtacgtt cagcgggaag tggcgacgac caatttccac gcccggcgtg gtggttggga
     2041 tcagcgcaca ggtaatgcct aaatcttctg caccgccgag taatttttcc gggtcggaga
     2101 gtttaaacgc cagcccaagc acggtcgcaa tcggtgccag cgtaatgtag cgtttgttcc
     2161 aggtcagacg catccccagc acctgctggc cctgccattc gcccatgcag acaatcccgg
     2221 tgtccggaat cgcgcccgca tcggaacccg cttccgggct ggtcagtgca aagcagggga
     2281 tctcctgacc acgcgccaga cgcggcagat agtgatcttt ctgctcgtca gtgccgtaat
     2341 gttgcaacag ttcgcccggg cctaatgagt ttggcacgcc gacggtaatc gccaggatcc
     2401 cgctcacgcc ggagagtttt tgcagcacgc gagactgggc ataagccgag aactccagcc
     2461 cgccgtactc ttttttgatg atcatcgcga agaaacgatg ctctttaagg tacgcccaca
     2521 actccggcgg cagatccgcc agctcatggg tgatctggaa atcattcgcc atccggcagg
     2581 cttcttctac cgggccgtcg agaaacgctt gctcttcggc ggtcaggcgc ggctgcggat
     2641 agttatgcag ctttttccag tccggcttgc cctggaacaa gtcgccctcc caccaggtgg
     2701 tgcccgcatc aatcgcttct ttctcagtgc gcgacatcgg cggcatcacc ttacggaaac
     2761 cgcgaaatac cggcgcggaa atcatcgact tacgcatagg cgcaaagtta aatggcacga
     2821 ggataatggc cagaggcacc agtacccacg ccgaccacag accagcaacg ccgagggcgg
     2881 ctgtccaggc gagcaaaatc agactgctga taaataagct cacgcggtga tagaacaacg
     2941 cgccgagcag gacaaccgta gcgagaatac tcaaaatcat cataacgaaa agccccttac
     3001 ttgtaggagg tctgaccact tgtgatgata tggttgtagt ggatgtaaaa acatttagca
     3061 atgtgtttac aatataatta caacaaagct cacattgttg ctgtttttat ccgcacttca
     3121 ggtcaaaaag tcctggtcat agcacctgcc cgtacttctc gcttttggcg gtatccggta
     3181 cactgcattt tgtctattac atttatgctg aaggatatcc tcatgtacca ggatcttatt
     3241 cgtaacgaac tgaacgaagc ggcggaaacg ctggctaact ttttaaaaga tgacgccaat
     3301 attcacgcca ttcagcgcgc ggcggtcctg ttagcagaca gctttaaagc cggtggcaaa
     3361 gtgctttcct gcggcaacgg cggttcccat tgcgacgcta tgcactttgc cgaagagttg
     3421 accggtcgct accgtgaaaa ccgtccgggc tacccggcga ttgctatttc tgacgttagt
     3481 catatttcct gcgtcggtaa tgatttcggt ttcaatgata ttttctcccg ctacgttgaa
     3541 gcggtaggtc gcgaaggcga tgtactgctg gggatctcca cctccggtaa ctctgcaaac
     3601 gtgatcaaag cgatcgcagc ggcgcgtgag aaggggatga aagtgatcac cctgaccggt
     3661 aaagacggcg gcaaaatggc tggcacggcg gatatcgaaa ttcgcgtacc gcactttggt
     3721 tatgccgacc gcattcagga gattcacatt aaagtgatcc atatcctgat ccagttgatt
     3781 gaaaaagaga tggttaagta agtctggcgt aggccggata agacgtttac gccgcatccg
     3841 gcatttgtgc gctgatgcct gatgcgacgc tgacgcgtct tatcatgcct acaaatctgt
     3901 acgcgaaccg taggccgaat aatgcgttca cgccgcatcc gacctgaaaa ttcttaaatc
     3961 aatcttcgcc gggggccatg cgctcccgct gttgtggagg ttacccatgt gcgaattgct
     4021 cgggatgagc gccaacgtcc ctaccgatat ctgctttagt ttcaccgggc ttgtacagcg
     4081 tggtggtgga accgggccac ataaagatgg ctggggcatt accttttacg aaggtaaagg
     4141 ctgtcgcaca tttaaagatc cacaacccag ctttaattcc cccatcgcca aacttgtcca
     4201 ggactacccg ataaaatcct gttcggtggt ggctcatatt cgccaggcta atcggggcga
     4261 ggtggcgctg gaaaatactc acccgtttac ccgcgagtta tgggggcgta actggactta
     4321 tgcccataac gggcaactga cgggctacaa atcactggaa accggcaact tccgcccggt
     4381 cggtgaaacc gacagcgaaa aagccttttg ctggctcctg cataaattaa cgcagcgtta
     4441 cccgcgcacg ccgggcaaca tggcggcagt gtttaaatat atcgcctcac tggcggatga
     4501 actgcgggcg aagggcgttt tcaacatgct gctttcggac ggtcgctatg taatggcgta
     4561 ttgctcgact aatttacact ggatcacccg ccgcgcgccg tttggcgtgg caacgttgct
     4621 ggatcaggat gtggaaatcg acttcagctc gcagaccaca ccgaatgatg tggtcacggt
     4681 gattgcgaca cagccgctga cgggcaatga aacctggcaa aaaattatgc caggcgaatg
     4741 gcgcttattt tgcctcgggg agcgtgtagt ttgatgccag ttgtggctgc accacttcgt
     4801 ggcttaacgg cttgctgacc acgtaacggc cattgaccac agaaacggtc ggtggcttac
     4861 gggtttgctc aaagtagtcg tagcccggct tcagctgctc ccagaagtcc ttaaagttgg
     4921 aatatttatg gcgcttcata ttggcgtcgg tcatgcggaa tgggtaaata ctcacctgca
     4981 cgctcggctg accaaacacc agcgcaccag taacgaactg gaagatctca tcaataccct
     5041 gattggtcat cgcgtagcag ccgatggaaa cacaatcgcc gtggatcatc agatatttcc
     5101 cttcataacc atgcgcacgg tcataggcat tggggaaacc aatattaatc gctttgtagt
     5161 aacggctgtc tggttttaac tgattacgct ggacgctata aaacccttcc gggcttttga
     5221 aatcgccctg acgctgtttt ggccctaagc cgccggaata tttacagatt ttatagctgt
     5281 cgagcagttg atattgctcg cccattttga cgtagagatc gagcgtacgt tcttccttga
     5341 agatctggat gtagacaggg gatcccatca actgctgctt atactctttg ctcactggcg
     5401 tggtcgagct actgctgccc agcaaaccgg caaacgaaac gcacgggatc aacaacatcg
     5461 caagaattaa tgcgatttta cgcatactgc ttattccttg ataaaacggt tacacacgcc
     5521 aggacggcaa aatggatccc aaatcggaat agtctggatt tggaaggctc acattatcac
     5581 caaaagagtt ttacgcaagc ctgtcggcgc agggtttaca aatttagcac aggggcagtt
     5641 ttcaatagtc cgaaaagcgc atgtatcatt tatttcctaa gattatgaaa cctcaatagt
     5701 aatatgcaaa taagcaatta ataatcccgt ttactcctcc ggtcttttaa aattctcacc
     5761 ttcattcagc cggtttatgt ctttcatgtc ttcgttcttg ctcggcagat ttctgcatcc
     5821 aggcgtgttt tcgttatgcg ttttgctccc tttattcgcc agcgccacca catcacatat
     5881 ctctttcagc tacgccgccc gccagcggat gcaaaaccgt gcgcgtttat taaaacagta
     5941 ccaaacccat ctgaaaaaac aggccagcta tattgtggaa ggcaatgccg aaagcagaag
     6001 ggcgctacgc cagcacaacc gggagcagat aaaacagcat ccagaatggt ttcctgcgcc
     6061 gctcaaggcg agcgaccgac gctggcaggc gctggcggag aacaatcact ttttaagcag
     6121 cgaccatctg cataacatca ccgaagtggc gattcaccgt ctggagcaac agcttggcaa
     6181 gccttacgtc tggggcggca cgcggcccga tcaaggcttc gactgtagcg ggttggtttt
     6241 ttatgcctac aacaagatcc ttgaggcgaa gctcccgcgc acggccaatg agatgtatca
     6301 ctatcatcgg gcaacgattg tggcgaataa cgacctgcgc cggggagatt tgctgttttt
     6361 ccatatccac agccgcgaga tagccgatca tatgggcgtg tatttgggcg atgggcaatt
     6421 tatcgagtcg ccacgtaccg gcgaaaccat tcggataagc cgattagccg aacctttctg
     6481 gcaggaccat tttttgggcg cgcgcaggat tttgacggaa gagacgattt tgtaggccgg
     6541 ataaggcgtt tacgccgcat ccggcggttg tgcgcagatg cctgatgcga cgctgacgcg
     6601 tcttatcatg cctacaaatc tgtgcgtttt tgcgagccgc tttcctgtta ttccatcacc
     6661 tcattgccga cctttccttt tcttcttacc gttgagaaaa aggagtcgcc atgtctgaat
     6721 atcgtcgtta ttacatcaag gggggaacct ggtttttcac ggtgaattta cgcaatcgtc
     6781 gaagccaact cttgaccacc cagtaccaga tgctccgtaa cgccattatt aaagttaagc
     6841 gagacaggcc ttttgaaatc aacgcctggg tcgttttgcc agagcatatg cattgcatat
     6901 ggacattacc tgaaggcgat gatgattttt cctctcgctg gcgggaaatt aaaaagcaat
     6961 ttacccatgc ttgtggattg aaaaatatct ggcaaccacg tttttgggag cacaccatcc
     7021 gcaataccaa agattatagg catcatgttg attatattta tataaatcca gtaaagcatg
     7081 gttgggtaaa gcaagtgagt gattggccat tctcaacgtt ccatcgcgat gtcgcgcgag
     7141 ggttatatcc catcgattgg gcgggggacg taacggatat aaatgctggc gagcgtatca
     7201 ttttataatt atgcgcagat gcctgatgcg acgctggcgc gtcttatcgt gcctacaaac
     7261 ctgtgccgga tcggtaggcc ggataaggcg tttacgccgc atccggcggt tatgcgcaga
     7321 tgcctgatgc gacgctgacg cgtcttatca tgcctacaaa cctgtgcctg aacagtagga
     7381 cggataaggc gttcacgccg catccggcag ttgtgcacca atgccggata cttacatcaa
     7441 cgcccccata atcttcaact ccagctcgtc cggcacttcg ttatacgaca gcacatgcag
     7501 ccccggtgca aacaaccttg cgtagcgcgc cagcaaagga cgcagctgag gcggcaccag
     7561 cagcaccggg tctttccccg ccgctttcat ctgctccttg acctgtggca tcgtactctg
     7621 gaactggttg agcatattcg ggtcgactgg cacgctgtca agcatcactt tcccgccctg
     7681 ttgcgcctga ttcaccacat tggtcagcag attttccagt tcattattca gcgtatacac
     7741 cgtcagctcc tgcttgcgaa cgaacggatg ggtaatgctg cgccgcagcg ccaggcgcac
     7801 atcagctgcc agcagaatat gatctttggt caccgcgcta ctggcgacca gcacggtggc
     7861 gatggtgacg atatcgcgca gggaaacgcc ttcggtcagc agcgcccggt acactttcag
     7921 caactggctg taattgagcg ccgcgcttaa atcttccgcc aggcgcggtg ccatcgacgc
     7981 caaacggtta tgcaactgcg taatatcatc atagttaaac aaatcaggaa tatagctgcg
     8041 cacaatcttg ttcacatggg tagcaatcac gctggcgctg tcgatcacct gataccccat
     8101 attcagcgcc ttcgctttct gcgcaggctg gatccaggtc accggcatcc cgtacgccgg
     8161 gtcgttcccc agcacgccgt ctatctcgcc gtaggtttcg ctggagggca gcgccatcag
     8221 tttatccgcc ggaatatccg cttcatcagc cttaataccg ttgatgaaaa tggcgtactg
     8281 gctgggcttg aggcggaaat tttcccgaat gcggatctcc ggcagcagca cgccgttgcc
     8341 gtcggatatc acctgtcgca cgccgcggat ccgctgggtg agcgggttgc cctgggcttt
     8401 gtccaccagc gccaccagtt tgtagccgag gcttaaactg ataggctcaa tcagcggaat
     8461 agtctcccaa ctgacctgtt gctcgctggt ttcggtgatg gtgcgggtca gcgtttcgag
     8521 gcttttctcc tccgcctccg ccgcctgcgg gcgtttgctc atccgccagc cggtaaagcc
     8581 aagcagggcg ctgaacagca aaaacggcag gtgcggcatc cccggtacca ccgccagcac
     8641 gaacatgatc ccggtagcgg tgtagagcac cgacgggctt gccagcagct ggtggcgcac
     8701 gtcatgggcg atatcgccgt tgtcgctgac gcgggtgacg ataatcgccg ccgcggtgga
     8761 gagcagcagg gaagggatct gcgccaccag cccatcgccg atggtcatta gcacatattg
     8821 ctggaaggcg gcgtcggcgc tcaggttgta tttgaagatc ccgatacaga cgccgccgat
     8881 caggttgatt gccagaatca tcatcccggc gatggcatcc ccgcgcacaa actttgacgc
     8941 cccgtccatc gcgccgtaga aatcggcctc gctggcgaca tctttacgcc gggtttgcgc
     9001 ctgcgcctgg ttgatcaacc cggcgttaag gtcggcgtca atcgccatct gtttgccggg
     9061 catcgcgtcc agggtaaagc gggcagaaac ctcggaaata cgctcggccc ctttggtgac
     9121 gacgataaag ttgattgtca acagcgtcag taaatccgag cgtgaaagca ttatcgccgc
     9181 gctgcacggg cagtcgattt tcagtggcgg cggattgtcg ccgttaaata aaatcagccc
     9241 gtctcatccg ccaaaacctg ccaccgtggc ggtgccggaa gagacggaga aaaaagcgcg
     9301 agacgtcaac gagaaaacag cgctgctgaa gaagaaatcc gccaccgagc ttggcgagct
     9361 ggcaaccagt atcaacacca ttgcccgtga tgcgcatatg gaagcgaatc tggagatgga
     9421 gattgttccc cagggattgc gcgtgctgat taaagatgac cagaaccgca atatgtttga
     9481 acgcggcagc gcgaagatta tgccgttctt taaaacgctg ctggtggagc tcgcgccagt
     9541 gttcgactcg ctcgataata aaattattat taccgggcat accgatgcga tggcctacaa
     9601 aaacaatatc tacaacaact ggaacctttc gggcgatcgc gcgctttcgg ctcgtcgggt
     9661 gctggaagag gccggaatgc cggaagataa agtgatgcag gtaagcgcaa tggcggacca
     9721 gatgctgctg gacgcgaaga atccgcaaag tgcgggtaat cggcgcatcg agattatggt
     9781 gctgaccaaa agtgcgtccg atacgctgta tcaatacttt ggtcagtatg gggataaagt
     9841 ggtgcagccg ctggtgcaaa agctggataa gcagcaggtg ctttcgcagc gaacgcgcta
     9901 aatgctgaat ctttacgcat ttctcaaacc ctgaaatcac tgtatacttt accagtgttg
     9961 agaggtgagt aatgcgtaaa atcattcatg tggatatgga ctgctttttc gccgcagtgg
    10021 agatgcgcga caatcccgcc ctgcgcgata tccctattgc tattggcggc agccgcgaac
    10081 gtcggggggt gatcagcacc gccaattatc ccgcgcgtaa atttggcgta cgtagcgcta
    10141 tgccgacagg gatggcgctc aaattatgcc cgcatctcac cttgcttccg gggcgctttg
    10201 acgcctacaa agaagcctca aatcatatcc gtgaaatctt ctcgcgctac acctcgcgca
    10261 ttgaaccgtt gtcactggat gaggcttatc tcgacgtcac cgatagcgtc cattgccacg
    10321 gttctgcgac cctcatcgcc caggaaatcc gccagacgat tttcagcgag ctgcaactga
    10381 cggcgtctgc gggcgtggca ccagtaaagt ttctcgccaa aatcgcctcc gacatgaata
    10441 aacccaacgg ccagtttgtg attacgccgg cagaagttcc ggcattttta caaaccctac
    10501 cgctggcaaa aattcccggc gtcggcaaag tctcggcggc aaaactggaa gcgatggggc
    10561 tgcggacctg cggtgatgta caaaagtgtg atctggtgat tctgcttaaa cgctttggca
    10621 aatttggccg cattttgtgg gagcgtagtc aggggattga cgagcgcgac gttaacagcg
    10681 aacggttacg aaaatccgtc ggcgtggaac gcacgatggc ggaagatatt catcactggt
    10741 ctgaatgtga agcgattatc gagcggctgt atccggaact tgaacgccgt ctggcaaagg
    10801 taaaacctga tttactgatt gctcgccagg gggtgaaatt aaagttcgac gattttcagc
    10861 aaaccaccca ggagcacgtc tggccgcggc tgaataaagc tgatctaatc gccaccgcgc
    10921 gtaaaacctg ggatgaacgc cgcggcgggc gcggtgtgcg actggtgggg ctacatgtga
    10981 cgctactgga tccgcaaatg gaaagacaac tggtgctggg attatgaaca acatacaaat
    11041 aagaaactat cagcctggcg attttcagca actatgcgct atttttatta gagcggttac
    11101 gatgaccgcc agtcagcact attctccaca acaaattgcc gcctgggcgc agattgacga
    11161 atcttgctgg aaggagaaac tcgcgaaatc acaagtgtgg gttgcggtca ttaatgcaca
    11221 accggttggt tttatttccc gcattgaacg tcatatcgat atgttatttg ttgaccctga
    11281 atacacccgc cgcggggttg ccagcgcctt gttaaaacct ttgattaagt ctgaatccga
    11341 acttacggtg gacgccagca taaccgcaaa accctttttt gaacgttatg gttttcagat
    11401 agttaagcag cagcacgttg aatgccgggg agcgtggttt actaattttt atatgcgata
    11461 taaaccgcaa cattaaatcc agcttgcaat gaaaataacg cccgcctggt atgtgcgcgt
    11521 tttcttattc cacaaactgc aaggaggtaa atcatgacaa atcctttatc aatgactctt
    11581 tgcagacctt tcc
//
LOCUS       DAOFQD010000146        11547 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.146, whole genome shotgun sequence.
ACCESSION   DAOFQD010000146 DAOFQD010000000
VERSION     DAOFQD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.146"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(55..1812)
                     /locus_tag="QBF46_004054"
     CDS             complement(55..1812)
                     /locus_tag="QBF46_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDQ6676168.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     HVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISRLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQEEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(1828..2724)
                     /locus_tag="QBF46_004055"
     CDS             complement(1828..2724)
                     /locus_tag="QBF46_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDQ6676169.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRSPERKGDEVTLRIRTWWG
                     IVICFSMVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFISLTGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHTAWLLMLPT
                     INIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(2724..3329)
                     /locus_tag="QBF46_004056"
     CDS             complement(2724..3329)
                     /locus_tag="QBF46_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDQ6676170.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRSLIFGLWGL
                     AVAIYPQWMQWNNLLWGIASILLIWTAINRCRSVLLMSAER"
     gene            complement(3500..5716)
                     /locus_tag="QBF46_004057"
     CDS             complement(3500..5716)
                     /locus_tag="QBF46_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDQ6676171.1"
                     /translation="MQLILNNFFSAATAEDKQQVEQAINSSVNLVPFGLSASDWKVHR
                     GDLVVEGNIESNQKLIVLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGS
                     INANGLVFIDSYYDNPSTIKGSINARGIFINDIIAPVVVSSTNSEFMVRASDKNDTEN
                     VKKALMIINPDAYHWGLINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELV
                     QELQMLDEGKVAAFEGRDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMAR
                     YMPDNEILELTDQQLGYQPVVLGLLNREPLSVEIMTRMSRLPDGVVPLNLALRENLPL
                     DIVMTLAKRDWDMIIQELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQL
                     ANDSSNNVVTSLAFKLAEMKHHGQLLRMTPQESDKVAVYLYQKFENDDDLIGALFLAL
                     PDNLQFNFVKRMEKKSPAYFCCRDMQIIHSDAALQRLLTRFNDPEGWSNLAKNQYLST
                     AMKQKIWQRALSHRKNNPKADSAAYETSADMILSELISYGEVDDQMLLNATSLIRSED
                     WDFLESALISWDNLPAVVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALD
                     PDALAQLDVLAKQPDRIWWSTLAKSNLTFFKFGALNNRHTPPAVLAAEIDPEWWIVAM
                     NNPRFPVDVLKARLKRDPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(5871..6731)
                     /gene="pdxI"
                     /locus_tag="QBF46_004058"
     CDS             complement(5871..6731)
                     /gene="pdxI"
                     /locus_tag="QBF46_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDQ6676172.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(6940..>11547)
                     /locus_tag="QBF46_004059"
     CDS             complement(6940..>11547)
                     /locus_tag="QBF46_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDQ6676173.1"
                     /translation="GIQIDGDQAIVNNEGESTITNGGTGTQINGNDATANNSGKTTVD
                     GKDSTGTKIAGNIGIVNLDGSLTVTGGAHGVENIGDNGTVNNKGDIVVSDTGSIGVLI
                     NGEGATVSNTGDVNVSNEATGFSITTNSGKVSLAGSMQVGDFSTGVDLNGNNNSVTLA
                     AKDLKVVGQKATGINVSGDANTVNITGNVLVDKDKTADNAAEYFFDPSVGINVYGSDN
                     NVTLDGKLTVVSDSEVTSRQSNLFDGSAEKTSGLVVIGDGNTVNMNGGLELIGEKNAL
                     ADGSQVASLRTGYSYTSVIVVSGESSVYLNGDTTISGEFPLGFAGVIRVQDKALLEIG
                     SGATLTMQDIDSFEHHGTRTPELTYADSGAKIVNKGTVEIQNLGFAFVTGENTTGINS
                     GTISLLQNGKDPAPSPIVLLATNGGSATNAGTITGKVTEQHSVFNKYSTGTSNSFIFN
                     NDVSSITGLVAQSNSTIINTDSGIIDLYGRGSVGMLAIADSTAENQGKITLDSMWVDA
                     NDTTAMRDIASNSAIDFGTGVGVGTDSYSGAGKNATAINQLGGVITIYNAGAGMAAYG
                     ASNTVINQGTINLEKNGNYDDSLAANTLVGMAVYEHGTAINDQTGVININVGTGQAFY
                     NDGTGTIVNYGTICTFGVCQSGNEYNNTDDFTSLIYTGGDTITRSGETVTLNKSAAVT
                     DKLAGNVVNSGTLSGDQITVSSGLLENTSGGIINNLVKLDKGAVIKNAGVMTNNVDVS
                     GGILNNAGEMTAQITMNAGADSSLVNNTGTINKIVQNAGVFNNSGSVTGRMMSAGGVF
                     NNQTDGAIMRGAALTGTAVANNEGTWNLGSSSEGNNTGMLEVNNNSAFNNRGEFILDN
                     DKNAVHINQSGTLYNTGHMNISNSSHNGAVNMWGGNGRFINDGTIDVSAKSLVVSANN
                     AGDQNAFFWNQDNGVINFDHDSASAVKVTHSNFIAQNDGIMNISGTGAVAMEGDKNAQ
                     LVNNGTINLGTAGTTDTGMIGMQLDANATADAVIENNGTINIFANDSFAFSVLGTVGH
                     VVNNGTVVIADGVTGSGLIKQGDSINVEGMNGNNGNSSEVHYGDYTLPDVPKPNTVSV
                     TSGSDEAGGSMNNLNGYVVGTNVNGSAGKLKVNNASMNGVGINTGFTAGTADTTVSFD
                     NVVEGSNLTDADAITSTSVVWTAKGSTDASGNVDVTMSKNAYTDVATDASVNDIAKAL
                     DAGYTNNELFTSLNVGTTAELNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVG
                     NGLAFNVVAKGDPRAELGNNTEYDMLALRKTIDLSESQTMSLEYGIARLDGDGAQKAG
                     DNGVTGGYSQFFGLKHQMSFDNGMNWNNALRYDVHNLDSSRSIAFGNTNKTADTDVKQ
                     QYLEFRSEGAKTFEPSEGLKVTPYAGVKLRHTLEGGYQERNAGDFNLNMNSGSETAVD
                     SIVGLKLDYAGKDGWSASATLEGGPNLSYAKSQRTASLAGAGSQHFNVDDGQKGGGIN
                     SLTSVGVKYSSKESSLNLDAYNWKEDGISDKGVMLNFKKTF"
BASE COUNT         3045 a         3038 c         2428 g         3036 t
ORIGIN      
        1 aacggggcca gcaacaataa ccagccagcg ccttgtagca atacgttccg acgttcagtt
       61 atcacgacgc accgccatcg aaaccgtaaa aataccccac tcatcaatcc gttgtgtgca
      121 tttatcaaat ccggcatcac gcacgagtga atccatctcc tcttgcgaac gcacgcgcat
      181 gacccacggt ttaccatctt tatgactggt taacaccccg gcaatcagct ccagttgtgg
      241 gtgccacggc tgcccggtgt agaggagaat gccgcccggt tcgatggcat tggcaagacc
      301 tgcgagtgag ttttttacct gctcattttc gggaaaaagc tcatacaggc cagagacaat
      361 cgccagcgta ggccgcggag ttaacgcgct gagttcctcc gggttaaacg catcgccctg
      421 ttcaaaacgc acccgcccag acattccccg ttgagcaatc atctcttgcc cctgtgcaac
      481 atttaactcg ctgtaatcac gtaacaaaat atcgcttacg gcaggctcgt ttgccagcgc
      541 atccagtaca tagcgcccat gccctgcggc aatgtcaacc acgcggacgg ctaaaccttt
      601 ggcgtggaga tcggcaacgg cttgtttaat cagtatttgt aaatgggttt tgcgctggcg
      661 aataccgcgc cagccaacac tgttcaggta gattttgtcg actaaacgcc cgaatgcgtt
      721 actaccttgc ggttgatttt gatagacata atccagcgaa ctgcctgaat caaagccggt
      781 gctcattccg agatgcaggc ccgcagagtg cgccccgaac aatttcatcg ccttacgcat
      841 aaagcgatac gccaaatcaa ccggcgataa tggcacgggt ccaccagaaa ggagccgcca
      901 tcgatccgct gatggtccag tgcagtcttc atgctgataa tcaaattttt gcgatttgtt
      961 agcatataag cgactaataa agctttgcat tttttcaaat gccagcgccc tgttctcttc
     1021 acccaacgtg tcgtgataaa agcctggcag cagatgcagc tctttgagag ggctacgtaa
     1081 acgctgataa aaatcgatct gcggttggcg atgcaccaca tagtcatcgc ctgatatcag
     1141 aagttgcgtg gggagcgtaa tcgccgccgc atcgctaaca atacgttcag acgttttata
     1201 gagatcgagc aagatattga cggcaatcgc ccgtgtgatc agcggatcat tattgaaact
     1261 cgccacccgt tgccgatcgt gggtcaaata gcgtcctttc acatacgaat taataaaaaa
     1321 caaaccacgc agacgatgcc ataacgccag cgcaggacgt gccagcggca catacaattt
     1381 aaccttaaag gccggagaag ccagcaccag cccgcgaatt gcaggcgcat aatcatgaac
     1441 ccaagttgca accagcactg cgccgacgct ttgcgcgatc acaaccacct cttccagtcc
     1501 gacatggctg tcgctggcag caaaacggac aaattcatcg acatcctgca ctgaacgcgc
     1561 gagagatggg ctataaccac gcggccccga agtttttcca tgccctcggg catcccaggc
     1621 ataaaaagca gtatctggca tcgccagttc atcaacgata tgttgtagac gaccagaatg
     1681 ttcatgaccg cgatgaaata agacgatgac ctttttcgcc ccgggctgta aagcgggcca
     1741 gtgccgataa aacaacgctg tattatcact ggtggtaaaa aaatgttccc cagggatgcg
     1801 tgaattttcc attttctcta ttttccttca gtaacagcag tagcgaataa aataaaaaaa
     1861 taccggggcg gtgaaaatta atgagtcaat cctgtcaagg agtccgccat gtcctggtaa
     1921 tagttttcca ctatctttaa caccaatatc tcgtttgatg gctgacatca ctacatcgcc
     1981 gcaaaaacca ctaataccaa ttaacaaacc cgctaataat gcctgtaatg tatttagcgg
     2041 tgtcagcaac ggtccaataa tcagtgacgc aatcatggtg gtgattacgc cccccaccag
     2101 accttccagc gtttttcctg ggctgacttt ggggaccact tttcttctgc cgcaggattt
     2161 tccccataaa tactgtgcaa tatcgtttga ctcggttaac gccaaaagaa ataacaccag
     2221 taacgccccg ccctggatat ttatggttgg caacattaat agccaggcgg tatgactcaa
     2281 ggcgaaaacg gtcattatcc agccccaaaa aatggcgctc acagtatgca gaaaaccgga
     2341 gggatcaccc acaaaaactc gccaggtggc taatatcaga aacccagtca aagatataaa
     2401 taacagaaac agctcaaagc aattaaaacc gataagcaaa tagttaagag gaataaccca
     2461 atataaccaa cgcggaaaat gtacggatat aagcgtacaa tattctttca atgccagaaa
     2521 acttatcagc gcaaaaaacg tcaacgtcat ccagcgtgga cctgaaatca ccattgaaaa
     2581 acaaatgacg ataccccacc aggtacgaat acgtaatgta acttcatccc cttttctctc
     2641 cggtgaccgc catacccgaa aacggtttat cagcgttgct aaaattaaca acgcgaaaag
     2701 tgttgccaga gatttttcca gcattatctt tcagcactca tcaggagcac actccgacaa
     2761 cgattaatcg cagtccagat aagcagaatt gaagcaatgc cccataaaag attattccat
     2821 tgcatccact gtggataaat ggcaacggcc agcccccaca gaccaaatat taacgagcga
     2881 tcacttttgc caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa
     2941 ccgcaaaact cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt
     3001 tccggtaaaa ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca
     3061 ttcaaaatcg cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc
     3121 gcattgagcg ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt
     3181 gccaccaaca ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg
     3241 tgattcgctg taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga
     3301 aagagcggtt ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat
     3361 ggtgcatcaa ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat
     3421 atatcaaggt attggggact cactgtcagg aatgattatc cccaattaat caaatagtta
     3481 agaaatatta cgttgtggat caatacaact catcaagttt tctcatcgct tgttcccgta
     3541 ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa
     3601 gctctaacgc cagcaaagga tcacgtttca gcctcgcctt taatacatca actggaaaac
     3661 ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc agtactgcag
     3721 gcggtgtgtg gcggttgtta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca
     3781 gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat
     3841 cggggtcgag tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt
     3901 cctgcctcag aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct
     3961 tgagaacaac agcgggtaaa ttatcccaac taattaatgc actctctaaa aagtcccagt
     4021 cttccgaacg tatcagagag gtggcattta atagcatctg gtcatcaact tcaccgtagc
     4081 tgataagctc agacaaaatc atatcagcgc tggtttcata tgcagctgaa tctgctttcg
     4141 gattattttt ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gccgtgctca
     4201 gatactgatt tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc
     4261 gttgtaaagc agcgtcagag tgaattatct gcatatcccg acagcaaaag taggcagggg
     4321 attttttctc catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata
     4381 acgcacctat tagatcatcg tcattctcga atttttggta taaatagact gcaactttgt
     4441 cactctcttg tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga
     4501 acgctaagct tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt
     4561 aggtgagttg tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc
     4621 catcaataat ggattcaggc aataaccacg catctttata aagctcctga atgatcatat
     4681 cccaatctct tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa
     4741 gattcaacgg aacgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga
     4801 gcggttcacg gttgagcaat cccagaacca caggctggta acccaattgc tgatcggtca
     4861 actccagaat ctcattgtca ggcatatatc tagccatact ttcaatcgtt tgttcatcat
     4921 tgctattaat aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc
     4981 gcattatggc aagatcaaat gtcgcaatgt ctcgtccttc aaatgcagca actttgcctt
     5041 catccagcat ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt
     5101 cttttttcat ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt
     5161 ctttcagagc atcttcatca tttattagac cccaatgata tgcatcagga tttattatca
     5221 tcagcgcttt ttttacattt tcagtgtcat ttttgtcact tgcacggacc atgaattcac
     5281 tatttgtcga agacacaaca acaggtgcaa ttatgtcatt gataaatatc ccacgcgcat
     5341 taatacttcc cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccat
     5401 ttgcatttat cgaaccggta attaacagtg gcgaatctgt aactatattt gttgccgtca
     5461 cgttaccgag aataacccat ggattagaaa gagagaaagt ggaaatattt cctttgactg
     5521 tcaaattgcc aagaacaatc aatttttgat tactctctat attaccttct accactaaat
     5581 cgccacgatg cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac
     5641 tgttgatagc ttgttcaact tgttgtttat cttcagcggt agccgcagaa aaaaaattgt
     5701 tcaatatcaa ctgcatttgc aacacatcga agttccgcct gtactggaag actcagcata
     5761 aacaggggaa gtaaaactat ccagccgcaa ttcctggcct taaacatatg cactccatgc
     5821 cattaaaaaa gaaacaaaag ggaaatgctt ctttcccttt tgtatattcg ttattctcgc
     5881 gaaataccat ccaatgtaga caacacttcc tcagaaagat gcaatttttc agcagccata
     5941 ttctcccgta aatgcgcaac agaagacgtc cctgggatca gcaaaatatt cggtgaacgt
     6001 tgtaacagcc acgccagcgc cacctgcatt ggtgttgcac ccaggctcgc tgcaacatcc
     6061 gacagcgtag aggattgtag cggtgtaaag cccccaagcg ggaagaacgg cacgtaggca
     6121 atgccgtcac gagccaaagc atcaatcatt gcgtcatcag cacgatgagc aatgttgtat
     6181 tcgttctgca cgcagacgat ttcggtaatc ttacgcgcct ctgcaacctg cgtcggtgta
     6241 acgttgctca gaccaatatg ttttaccagg ccttgttgtt gcatctctgc cagcacggtc
     6301 aggctggctt caatcgagcc ttccgctggg ccatgaccat cccccatcat aacgcgcagg
     6361 ttaaccacat ccagcacgtc caggccgaga ttacgtagat tatcgtgcac cgctttttgc
     6421 agctctgccg gagaaaaggc aggcaaccag gatgcgtcct ctccacgccg cgcaccaatt
     6481 ttagtgacaa ttgtcaggtc gtcagagtaa ggataaagtg cttcgcggat aatctgattg
     6541 gtgacgtgcg gaccataaaa gtcgctggta tcaatatgat tgacgcccaa tgccagcgcc
     6601 tcacgcagca cggttatagc gacgtggcga tctcgtgggg ggccaaaaac tccaggacct
     6661 gccagttgca tcgcgccata accaagacgg ttaacggatt ttgtaccgag agtaaatgta
     6721 ttgctgctca tcgcaccctg ccctgtaata aaacggatta agaaaaatca gtatagaaac
     6781 gaaaagagat aaataatgcg aaggcgtaaa caagtttact ggatgagttg ctctgttatc
     6841 ctgaaagaca ccaaaacaaa acgcgtcaag taccgcatcc ggcaataaaa aaaccgcaac
     6901 ctgtaccagc gttgcggttt aggtatcaca tgctaaaaat tagaacgttt tcttgaagtt
     6961 cagcatcacg cctttatcgc tgatgccatc ctctttccag ttgtacgcat ccagattcag
     7021 cgaactttct ttgctgctgt acttcacgcc gacgcttgtc aggctattga tgccgccgcc
     7081 cttctgaccg tcatcgacgt taaagtgctg actgcctgcg cctgccaggc ttgccgtacg
     7141 ctggctcttc gcgtagctca ggttcggccc gccttccagc gtagcgctag cgctccagcc
     7201 gtctttacct gcgtagtcca gtttcagccc gacgatgctg tccaccgccg tttcgctgcc
     7261 actgttcata ttcaggttaa agtctccggc attgcgctcc tgatagccac cttccagtgt
     7321 gtgacgcagt tttacacccg catatggcgt aaccttcagt ccttcgctcg gttcgaaagt
     7381 cttcgcccct tcgctgcgga actccaggta ctgctgtttc acgtcggtat cagccgtttt
     7441 gttcgtgttg ccaaatgcaa tcgagcggct gctgtcaagg ttgtgaacgt cgtaacgcaa
     7501 ggcgttattc cagttcatgc cgttatcgaa cgacatctga tgtttcaggc caaaaaactg
     7561 gctataaccg cctgtaacgc cattatcacc cgctttctgc gcaccatcac catcgagacg
     7621 agcgataccg tactccagac tcatcgtctg gctttcgctc aggtcgatag ttttacgcaa
     7681 tgccagcatg tcgtattcgg tattattacc taactcggca cgcggatcgc ctttcgcgac
     7741 aacgttgaac gccagaccgt tacccacttt cggcgcggca tctgccagca tactaaagcg
     7801 gttgcttaac acgcgcgctt cgcggaatac cgtggtcgcc tggctaccgc tgacctgttt
     7861 cagagcactg ttcagttcag cagtcgtgcc gacgttcagg ctggtaaaca gttcgttgtt
     7921 ggtgtaaccc gcatccagtg ctttcgcgat gtcattcacc gaggcatctg ttgccacatc
     7981 ggtgtaggca tttttgctca tggtgacgtc aacgttaccg ctggcatcgg tgctgccttt
     8041 ggcagtccat accacggacg ttgaggtgat ggcgtcagcg tcggtcaggt tgctaccttc
     8101 cactacgtta tcaaaactca cagtggtgtc tgccgtacca gcggtaaagc ccgtgttaat
     8161 ccccacgccg ttcatgctgg cattgttaac cttcagcttc ccggcgctgc cgttaacgtt
     8221 ggtaccgacg acatagccgt tgaggttgtt catgctgcca ccagcctcat cacttcccga
     8281 cgttacacta accgtattgg gcttcggcac atccggcaac gtatagtcgc cataatgcac
     8341 ttcgctgcta ttaccgttgt taccgttcat accttcaaca ttgatgctgt cgccctgctt
     8401 gatcagtcca gaacccgtaa ccccatcggc aatcaccacc gtgccgttgt taaccacatg
     8461 acctactgta cccagtacgc taaatgcaaa cgagtcattg gcgaagatat tgatggtgcc
     8521 gttgttttcg attaccgcat ccgccgtggc gttggcatcg agttgcatac cgatcatccc
     8581 cgtgtcagta gtgcctgcgg taccgaggtt gatggtgcca ttgttaacca gctgcgcgtt
     8641 cttatcacct tccatagcca cagcaccggt gccgctgatg ttcatgatgc cgtcattctg
     8701 ggcaataaag ttgctgtggg tgactttcac ggcactggcg ctgtcgtgat cgaagttgat
     8761 gaccccgtta tcctggttcc agaagaaggc attctgatcg ccggcattat tagcgctgac
     8821 taccagtgac ttcgcagaaa catcaatcgt tccgtcattg ataaaacgac catttccgcc
     8881 ccacatatta acggctccgt tgtgggaaga attactgatg ttcatgtgac cggtattata
     8941 aagcgtaccg gactggttga tgtgcacagc attcttgtcg ttatcaagaa taaactcgcc
     9001 gcggttattg aaagctgaat tattattaac ttccagcatc ccggtgttgt taccctcact
     9061 actacttccg aggttccagg ttccttcgtt atttgccact gcagtacctg tcagcgcagc
     9121 acctctcata atcgccccgt cagtttgatt attaaagacc ccgccagccg acatcatccg
     9181 ccctgttaca ctgccactat tattgaatac ccccgcgttc tgcacgattt tattgatggt
     9241 tccggtgttg ttcactaacg aactatcagc accagcattc atggtaattt gcgcagtcat
     9301 ttctccggca ttattgagga ttccaccgct aacatcgacg ttattcgtca tcaccccggc
     9361 atttttaatg acggcaccct tgtcaagttt tactaagtta ttgatgatgc caccgctggt
     9421 attttccaga agaccgcttg ataccgtaat ttgatcaccg gaaagcgttc cgctattaac
     9481 aacattccca gccagcttat cagtcacagc agcagattta tttagcgtta cagtttctcc
     9541 gcttcgtgta atcgtatcgc caccggtata gatcagtgag gtgaaatcat ctgtattatt
     9601 gtactcattc cccgattggc acacgccgaa agtgcagatt gtaccatagt taacaattgt
     9661 tcctgtgcca tcgttataaa acgcctgacc agtaccaaca ttgatattga taacacccgt
     9721 ctggtcgttg atagcagtac catgctcata aacagccatc cctaccagag tatttgctgc
     9781 cagactatcg tcataattac catttttttc gaggttaatc gtcccctggt taataactgt
     9841 attgctggcg ccataggccg ccatacctgc gccggcgtta taaatagtta taacaccgcc
     9901 caattggtta attgctgttg catttttccc tgcaccacta taactatcag taccaactcc
     9961 cacacctgta ccgaagtcaa tggcgctgtt gctagctata tctcgcattg cggtagtgtc
    10021 atttgcatct acccacatag aatccagtgt aattttaccc tgattttctg ctgttgaatc
    10081 tgctatagca agcatgccga cactaccacg accatacaaa tcaatgatgc cgctgtcagt
    10141 attgataatt gtgctattcg attgagcgac taaccctgtt atgctactga catcgttatt
    10201 aaaaataaat gaattcgatg tgcccgttga atacttgtta aatacgctat gttgttccgt
    10261 cactttacct gtgatcgtac ctgcattagt ggcgctccct ccgttagtag ccagtaaaac
    10321 aatgggagac ggtgccggat ctttaccatt ttgtaataac gagatcgtgc cactatttat
    10381 acctgttgta ttttcaccag taacaaaagc aaaacctaaa ttctgaattt caacagtacc
    10441 tttattaaca attttcgcac cggaatcagc ataagtaagt tctggggttc ttgtcccatg
    10501 atgttcaaaa ctgtcaatat cctgcattgt tagcgtagcg ccactgccaa tttccagcaa
    10561 agctttatcc tgtacccgaa taaccccggc aaaccccaga gggaattctc cgctgattgt
    10621 cgtatctcca ttcagatata ccgacgactc accactaacg acaataacgc tggtataact
    10681 atatcctgtg cgcaaggaag caacctgcga cccatctgca agcgcgtttt tctctccaat
    10741 cagttcaagt ccaccattca tattaacggt attgccatcg ccaatcacaa ccagacctga
    10801 cgttttctct gcgctgccat caaataaatt actctgacga gaagtaacct cactgtctga
    10861 tacaacagtt aactttccat ccagcgtcac gttattatca ctgccgtaaa cgttgatacc
    10921 cacggatgga tcgaagaaat attccgccgc attgtctgcg gttttatcct tatcaaccag
    10981 aacgttacca gtgatattca ctgtattcgc atcgccagaa acgtttatgc ccgtcgcttt
    11041 ctgcccgacc acttttagat cttttgccgc cagcgtcacg ctgttattgt tgccattaag
    11101 atctaccccg gtcgagaaat cgccaacctg catactgcct gccagcgaaa ccttcccact
    11161 gttggttgtg atgctgaacc ctgtcgcttc attgctaacg ttaacatcac ccgtattgga
    11221 tactgttgcc ccctcaccgt tgatgagcac gccaatcgat ccagtatcgg aaacaacaat
    11281 atctcctttg ttgttaaccg taccgttgtc accaatgttc tcaacaccat gcgcaccgcc
    11341 tgtaacagtc aggctaccat ccagatttac aatgccgata ttgcccgcga ttttggtacc
    11401 cgtggaatct tttccatcaa cagtggtttt tccactgtta ttcgcggtgg cgtcgttacc
    11461 gttgatctga gtgccggtgc cgccattggt gatagtgctc tcgccttcgt tattgacgat
    11521 ggcctggtcg ccgtcaatct ggatacc
//
LOCUS       DAOFQD010000147        11543 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.147, whole genome shotgun sequence.
ACCESSION   DAOFQD010000147 DAOFQD010000000
VERSION     DAOFQD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.147"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..389)
                     /locus_tag="QBF46_004060"
     CDS             complement(<1..389)
                     /locus_tag="QBF46_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="catalyzes th removal of D-alanine and attachment of
                     the murein lipoprotein to the peptidoglycan tetrapeptide
                     chain; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDQ6676174.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQMILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGR"
     gene            complement(451..1530)
                     /gene="cobT"
                     /locus_tag="QBF46_004061"
     CDS             complement(451..1530)
                     /gene="cobT"
                     /locus_tag="QBF46_004061"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDQ6676175.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMLGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(1542..2285)
                     /gene="cobS"
                     /locus_tag="QBF46_004062"
     CDS             complement(1542..2285)
                     /gene="cobS"
                     /locus_tag="QBF46_004062"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDQ6676176.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(2282..2827)
                     /gene="cobU"
                     /locus_tag="QBF46_004063"
     CDS             complement(2282..2827)
                     /gene="cobU"
                     /locus_tag="QBF46_004063"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDQ6676177.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            3015..3185
                     /locus_tag="QBF46_004064"
     CDS             3015..3185
                     /locus_tag="QBF46_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDQ6676178.1"
                     /translation="MIGVVTKADLASMEQISLVKCWLREAGAHNVLVTNAVNNHGVTE
                     LFALLHTEDVCR"
     gene            complement(3286..3615)
                     /locus_tag="QBF46_004065"
     CDS             complement(3286..3615)
                     /locus_tag="QBF46_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDQ6676179.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(3787..4845)
                     /locus_tag="QBF46_004066"
     CDS             complement(3787..4845)
                     /locus_tag="QBF46_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDQ6676180.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(5043..5516)
                     /gene="sbmC"
                     /locus_tag="QBF46_004067"
     CDS             complement(5043..5516)
                     /gene="sbmC"
                     /locus_tag="QBF46_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDQ6676181.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(5635..6801)
                     /gene="dacD"
                     /locus_tag="QBF46_004068"
     CDS             complement(5635..6801)
                     /gene="dacD"
                     /locus_tag="QBF46_004068"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDQ6676182.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            7010..8437
                     /gene="sbcB"
                     /locus_tag="QBF46_004069"
     CDS             7010..8437
                     /gene="sbcB"
                     /locus_tag="QBF46_004069"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDQ6676183.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFNVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAIIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(8480..8707)
                     /locus_tag="QBF46_004070"
     CDS             complement(8480..8707)
                     /locus_tag="QBF46_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDQ6676184.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(8721..9779)
                     /gene="yeeE"
                     /locus_tag="QBF46_004071"
     CDS             complement(8721..9779)
                     /gene="yeeE"
                     /locus_tag="QBF46_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDQ6676185.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHVSGLNQTLQHYSTEHNSIAETFNLSVWPLIAVL
                     LVITFWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(9958..11316)
                     /gene="plaP"
                     /locus_tag="QBF46_004072"
     CDS             complement(9958..11316)
                     /gene="plaP"
                     /locus_tag="QBF46_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDQ6676186.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(11306..11368)
                     /gene="yoeI"
                     /locus_tag="QBF46_004073"
     CDS             complement(11306..11368)
                     /gene="yoeI"
                     /locus_tag="QBF46_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDQ6676187.1"
                     /translation="MGQFFAYATVITVKENDHVA"
BASE COUNT         3148 a         3067 c         2635 g         2693 t
ORIGIN      
        1 cgcccggcct gaccgattcc aataggaaag acttctacag tattactgtc tggtgggtag
       61 taataaagac gcatctcagc gacgttaacc acaatccctt tacgaacagt ggcgggcaaa
      121 atcatttgct gcggaatggt gagttgcgag ccagacttcg gcaaaaaaac atcagcgccc
      181 gggttcgctt caagcatgtt acttaaccct tgcccgtatt gtgcggcaaa agtctccagc
      241 ggctgggtat tgtgatcagg aacagttaca gtaaacgact gccccactaa acggctaccc
      301 tctggaggta atggataagt taccgccagg ctagtatggc tggcaaaaag cagagcaaat
      361 gagcaaagaa tatttacacg acgcatcatg tccctttcct aagtcgcgaa agctatccgt
      421 taagtatagc ttttatcaga cttttcgttt ttaactgttc aaatcagaag tcgtattccc
      481 cggtagaaca atattactgg cagcaagttc gcccatgttg ttatatatcg cacaggcagc
      541 ttcgatgatg ggcatcgcca gagctgcgcc actcccctca cctaaacgca tctccatatt
      601 gagataaggc tccagcccca aatgcgacag cgcaatacgc gccccttttt ctgccgacag
      661 gtgagaagga atgagatacg gtttgattgc aggagacatc tggcaggctg cgagcgcggc
      721 agcataagaa agaaatccat ccagcaacac gggtaaaccg caggaagcag cgcctaacat
      781 cacgccagcc attccgacca aatcaaatcc acccactttt gccaggacat caacgccatc
      841 ctgcggattt ggttgattca acgtaatcgc ccggcgcaca acatcaattt tattagccag
      901 tttatctgtc ggcaggtttg cgccaatccc aaccacttct tcaggagccc ggccagtgat
      961 tgtgctgact attgccgctg ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa
     1021 cagcgtaaca ccgttttttg ccagctcccg cgtataacat atgacgtcca aaagcaactt
     1081 ttctgcctga tggcgactca ttgccggagc tgaagcaata ttgccactac ctcgagcaac
     1141 acgcatgttg ataagcccgg ggataggctc agcagtatca ataccaacat caattacgta
     1201 gacgttagcg cccgcttgtg ctgccagcac acacacgccg gttgttccgc gggtcatatt
     1261 ttcagcctga atggctgtca cttcttttgg ggaaatagcg accccttcct cccagacgcc
     1321 gtgatcggca cacatgacca gtaccgcttt tttgcccaca tgcggaacgc cattcaaccc
     1381 cagcattcct gccagttgta tggcaagcgc ctccagcctt cccaggctac caacaggttt
     1441 gagtaacccg tcgatatgcc gttgtgcacg cgacatagcg gcaggatcga tagcagggat
     1501 cgtattcagt aaatcggcaa gtgtttgcat gtcacgtctc tttatagcag tgccagcagg
     1561 aagaccagtt caccaagctc tatagcagct cccagcgtat cgccggtttg cccgcccagc
     1621 gtacgtttga gaagctgacc gaggatgaaa atcgccacca tcgtcaccac catagcagcc
     1681 acaccatgca tacccggcaa taatacggca gcaaaaatag cagctaagcc gagtgtgacg
     1741 caggtttgtc gcccatcaat tttgccaatg aacacattgc caagtccttc ttcccgggca
     1801 taacgatgac gatacatcaa taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc
     1861 agcgatgcaa ggatcggttc gccacgcaga gccaactcgc ttaataccag aatctttgcc
     1921 agtacaacaa aaatcaatgc cagaccgccg tgggtgccta aacggctatc acgcatgatt
     1981 tccaacatgc gatcgcggct acgtgcagaa aatacgccat cacaggtatc ggcgaggccg
     2041 tccaggtgga atcccccggt catcagcgcc agcacaagta cgctaaacag tgccgccagt
     2101 ggtacgccac accatgcctg cagcaccatg aagaccagcc cgctaatcgc gccaagcaat
     2161 aatccaatta aaggaaaagt aataataccg cgagaataat gctcgaaatc cagtccctgg
     2221 gaccagcgac gcggtacagg cagacgcgta ataaaagaga gcatcgccca aaataattta
     2281 ctcatttgat ttttactcca atacccgaaa ccaccaacca tacttcattt gccgcagcgg
     2341 ccagctgctg atttacccgc ccggcaatat cacgaaaatg tcgtgccaga cgactctccg
     2401 gcacaatccc cattcccact tcgttagtca ctaatacaac ctttgcggga caacgttggc
     2461 aggcagcaat caacgactga atctcagcat taatcgcctg ttccatcgcc tgataatccc
     2521 attcatcagg gtctttatcg ccgccataat caaacaacag attagtcacc attgtggtaa
     2581 cgcattccag caacacagct tcatgcgggt taatgtctgc atgaattaat tcatcaagat
     2641 gttgccaacg ctccaccgtg cgccagtgcg ccggacggcc ttgccgatga tgttctatcc
     2701 gtgcagccat ctcatcatca aggatttgcg aggtggcgat atacagaacc tgtgaagagt
     2761 ccccaataag cgcctctgcg tggcgactct tcccgctccg tgctccacct gtcaccaaaa
     2821 tcatcatacc gactcctgat tcaattgtat gatggtattc attgaacaga acaatggatc
     2881 ccccaccgtt gctgaccgca aaacatttac gcgattaata ctgcgcgtaa tataattaaa
     2941 aatccagcat tctcaatcca ttacgaaaga agatggcatt gtggaatagc cagacttcct
     3001 cgtgttaacg tccgatcatt ggagtagtca ccaaagcaga tttagccagc atggaacaaa
     3061 tatctttggt gaaatgttgg ttacgggaag ccggagcgca caacgtgtta gtaaccaatg
     3121 cggttaataa ccatggagtt acagaacttt ttgccttgct gcatacagaa gatgtctgtc
     3181 gctaatttcg acaggttctc agatgagaat gatgacggtg atacacagaa aagaatcaat
     3241 gagcaatgag ggttgccggg caaccctcat tgaataaaac aggaattact tcgcttcgcc
     3301 gtttttcatt tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag
     3361 ctcggcattt ttgatgatgt aatcgtcaac acgatcttca tagtcacctt tcatgctggc
     3421 gatgatgccc tggattgctt caacgctcat ccccggcttg atgtaatcgc tcaggttgtc
     3481 cagcagcagg acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg
     3541 ttgcagtttg ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg
     3601 cttggtagtt tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc
     3661 gctattcgtt agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt
     3721 ttcactatca taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt
     3781 caggaattat ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc
     3841 cgtctccatg ttcagccaca cataaccata gattggtgtt tccacaacct tcaggtcatg
     3901 gtggttattg agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc
     3961 aaaaaccggc tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg
     4021 catcatatgc tgggtatcac gaagtttttg cgcgttcaat aacacgaaat ggctggggcg
     4081 cgtggcccag tacgcattga tttgcaactc cagcatacaa accagattgc gattaatggt
     4141 ctggatacct tcatatatcg attttggaat acgagtttct ttgctggcgg gcgcaatcag
     4201 cccacgcatt ttcacggcat cggtcaggag tttttgtaga tggctttcca gacgtgggcg
     4261 ttcgagtaag ttcggtgaga atgcagattg ataaacccga ttatactcgg tcagactttt
     4321 cgccagttga atgcgccagt ggatgaacgc ccgttgtggc cagataccgg taaacaacat
     4381 tgcgagtaaa gagccgagga tcacatcgcc gcttcgccat aacgccgtat caatttcacc
     4441 tgtcggggaa cccacaacaa ttgccagcgt caccccaatc aataatcctt gatacggttt
     4501 cttgcccagt gccagccaac cgcaaagaaa catagccgcc gcgcaccaga ctaacatcag
     4561 cggtaacgaa attaactcca gttgcagagc gataagacct aatatcgaac ccaacaccgt
     4621 accgccaata cgttcgaagg ctcgagggac aacgttaccc cagaacgaga tgggccccat
     4681 aatcaccacc atggtgacca gcggccaggt actttccggg atagtaaaca ggcggataat
     4741 gagaaaagtg agcaggaatg ccagcgcgac ccgagtacca tgcacaatgc ggtaatggcg
     4801 gtatacccgg atttcaaacg ggcttaatga cttatcggca cgcacgcaga aactccgtaa
     4861 tgaaaattaa aagcgctcat aactcatctg tcggccagaa ggcgcgcctc tgaattatcc
     4921 gacggacaaa ccgggaacag tgtaatttcc cggcaggagc gaaaaaattg aaaggcgcat
     4981 cttactcttt tcgctttcaa aaaaagagtg gttatcgcgt taacacaccg ccctgagatg
     5041 gattagtgat gttttggctg caccgcaaca tacatttcga tatcccagta cccatcttcc
     5101 gcgccattgt tcaaataaac ctcaaaacag ggctttggta acatttcata agcactgtct
     5161 tgcaagaggc tattaaagaa ctgataccag ggtttagcaa aatcatcacc gactacacga
     5221 gccaccgcaa ccgcatactg accgcctgaa atttctgtca gaatgacgcc ctcactgttt
     5281 tcggggagcg taaagttatt cggtaccgtc acgacggtgt cgcagcgtaa tttttcggcg
     5341 ggtgtttcat ctggattgtc gtaatagaca gcaacccact ccttcggcac aatattttta
     5401 ctatctaccc acatcatcaa ctgctcaaaa cctttcttta ccgtctgctc ccacgggcca
     5461 acgagatgga aacctgcaac ggtacgttta tcttcctgct taatctcgta gttcatgacg
     5521 cctccattaa tactgttttt atatacagta tagttgcaat attaaaacca caaggaatga
     5581 gtgttgatta tgcgagcaga ctcgcactcc tgccagtctg ctgcaaaaga aaggtcaggc
     5641 cttatggtgg aaataatcac tcaggcgaga aaacatgctg ccttccccga cagattccag
     5701 ggtaaccagc ggccagtgcg ccacctgttt atcacggtcg taaagttcaa tttcccctac
     5761 ccgctgatgg gcgctaattg gcgcggtgag ttctttacca tcaagggtat atttggcttt
     5821 gatatgtgga atttcggctt tcggtagtac catccagaac tcttgttctg ttcccagggc
     5881 gatattttct ttatcgccat accatatgcg ttccgtaccg acctttttcc cacggtgcaa
     5941 aatttgcacc gtagtaaagt tttgttgccc ccaacgcagt aattttcttg cctcttcctc
     6001 acgacctttt gcgctgtcag cacccattac cactgcaatg agacgacgct gcccatctac
     6061 agccgaagca atgagattaa acccagcacc agaagtatga cccgttttca ggccgtcaac
     6121 attcatggtt ttatcccaca ataacccgtt acggttttgc tgggtgatac cgttccaggt
     6181 gaggcttttc tcactgtaca tatgataaaa ctcgggctcg ccgtggatga tagcacgaga
     6241 aagcacagct aaatcataag ccgagctatg ctgacctggc gcatccaggc catgcactgt
     6301 ttcaaaatgc gtatccttga gatgcagctt ctcggcatag ttgttcatca tttcaacaaa
     6361 ctgccgttgc ccaccggcaa tatagtcagc cagtgcaaca caagcgtcat ttccggaatc
     6421 cacaattaaa ccacggctta aatcacgtac cgatacgcga tcgccctctt tcaaaaacat
     6481 cagtgaagaa ccgacaaaca ccggattatc tttcgcccac gcatcgcgcc ccacggtgac
     6541 aatatcgtct ggcgtaatgc gatgactatc gatagcgcga tccacgacat aacccgtcat
     6601 cagctttgtc aggctggcgg gattgcgctg ttgatgctca ttacccgcgg tgaggatctg
     6661 accggtggtg taatccatca gtacccagga cccggcatga atctctggag gctgaggtga
     6721 aaaaggaatg ttttccgccg caaaaccaga cgataagtta aaaacgaaca aagaagcagc
     6781 aataataaga cggcgtttca acagcaaacc ctcaggagtt tcaaatagct gttcttttta
     6841 cggaaatact tatgaactgg ctggaataaa gtgcaagaaa atgtgactac cctctcattt
     6901 ttatctgaca tgatctgttg ccactcgctg ccaaattgtg gcgctaaagc tgattagcac
     6961 ggtgatattt gatactctgg cagacagcag aaataacgga tttaacctaa tgatgaatga
     7021 cggtaagcaa caatctacct ttttgtttca cgattacgaa acctttggca cgcaccccgc
     7081 gttagatcgc cctgcacagt tcgcagccat tcgcaccgat agcgaattca atgtcatcgg
     7141 cgaacccgaa gtcttttact gcaagcccgc ggatgactat ttgccccagc ctggagcagt
     7201 attaattacc ggtattaccc cgcaggaagc acgggcgaaa ggagaaaacg aagccgcgtt
     7261 tgccgcccgt attcactcgc tttttaacgt accgaagacc tgtattctgg gctacaacaa
     7321 tgtgcgtttc gacgacgaag tcacacgcaa cgttttttat cgtaatttct acgatcctta
     7381 cgcctggagc tggcagcatg ataactcgcg ctgggatttg ctggatgtta tgcgtgcctg
     7441 ttatgccctg cgcccggaag gaataaactg gcctgaaaat gatgacggtc taccgagctt
     7501 tcgccttgag catttaacca aagcgaatgg tattgaacat agcaacgccc acgatgctat
     7561 ggctgatgtg tacgccacta ttgcgatggc aaagctggta aaaacgcgtc agccacgcct
     7621 gtttgattat ctctttaccc atcgcaataa acacaaactg atggcgttga ttgatgttcc
     7681 gcagatgaaa cccctggtgc acgtttccgg aatgtttggg gcatggcgcg gcaacaccag
     7741 ctgggtggca ccgctggcgt ggcatcctga aaatcgcaat gccataatta tggtggattt
     7801 ggcaggagac atttcgccat tactggaact ggatagcgac acattgcgcg agcgtttata
     7861 taccgcaaaa accgatcttg gcgataacgc cgccgttccg gttaagctgg tgcacatcaa
     7921 taaatgtccg gtgctggccc aggcgaatac gctacgcccg gaagatgccg accgactggg
     7981 aattaatcgt cagcattgcc tcgataacct gaaaattctg cgtgaaaatc cgcaagtgcg
     8041 tgaaaaagtg gtggcgatat tcgcggaagc cgaaccgttt acgccttcag ataacgtgga
     8101 tgcacagctt tataacggct ttttcagtga cgccgatcgc gcagcaatga aaattgtgct
     8161 ggaaaccgag ccgcgtaatt taccggcact ggatatcact tttgttgata aacgaattga
     8221 aaaactgttg ttcaattatc gggcacgcaa cttcccgggg acgctggatt atgccgagca
     8281 gcaacgctgg ctggagcacc gccgccaggt cttcacgccc gagtttttac aaggttatgc
     8341 agatgaattg cagatgctgg cacaacaata tgccgataac aaagagaaag tggcgctgtt
     8401 aaaagcgctt tggcagtacg cggaagagat cgtctaattt gaagcctccg ccgctggtac
     8461 ggcggaggtc aatgttgagt caggcttttt gaacggtgat actccacgct gcatcgccaa
     8521 tctgctgata atcggtgatg gcatgccctt cttctgccgc ccactgtggg attgcttcag
     8581 ttgcctgggt gcaatcaaac tcaattacca actcatcgcc gctggccatt tctgccagtg
     8641 ctgcttttgc ttctatgagt ggaaacggac agacctgcgt caccacatcc agctttttaa
     8701 tcgccatctt tacaccttac ttaatttgcc gcagcagttg ccagtcgcgc cttacgctgc
     8761 ggtcgaacat acacaagcca ggacgcagtc cagactccga gaatcataaa tacaaggcca
     8821 atccagccct gccaggtcat cattgcagtc ataaccagtc cattaccgat agagcaacca
     8881 cctgcaatac tggcaccgaa gcccatcagt acaccaccta acccgctacg taatgttgtt
     8941 tgtgcatcag ctgcgcgaac gcggaactca cggctcgctt tggctgcaat aaatgacccc
     9001 acgaagatcc ctaacaccag gaaaacgccc cagttaatgt atttcacgtc acccgcgacc
     9061 agaaattgca gaatattggc cgttggggaa gtgattccta acccaaacat gcgtccggtt
     9121 gcttcactca ggggccaggc taaaagcgca atcaaaccga tgagtacagc agtgacaaag
     9181 ggatgccagc gtttttcaaa cagaatatga gcgatcccgg ttcggcgcgg cggtaaggtc
     9241 gcgactttga gttttggctt cttcaactct ttcatcacca cccagaaagt tatcaccagc
     9301 aaaacggcaa tcaacggcca cacagacaga ttaaacgtct cggcaatgga gttatgttca
     9361 gtactgtagt gctgcaaggt ttgatttaaa ccactgacat gtggagaacg catcaccgca
     9421 ctcatcacca tataagtgaa aagcgcgatc caactgccga tcaatccctc acccgcgcga
     9481 taccaggtcc cggtggcaca tccacccgcc agaacaattc ccagcccgaa gatataccca
     9541 cctataacag taccaagcca cgggaacgcc ccggcttcgt aagtcagtag gcccgcctga
     9601 attaacgcaa aaacccccac gctttgtacc gaaattgcaa tcagcaaggc gtaaaacatg
     9661 cgattatttt tcgcgatata catatcgcga aaaccacctg tcaggcaaaa acgcccacgc
     9721 tgcatgacaa atcccagcag agcaccacaa attagcccgc ttaatatcat tgaaaacata
     9781 aattaattaa ccagatgaat gttaatgggg aaattattca tgactggtgg aatgcagacc
     9841 aataaccaaa ttgtctaaat tagaacaaat ggttattaat gaggatgtcg ataaaaaaga
     9901 ataaaaaaac cggagccaaa tattcattcg actccggttt attattagaa aagatgatta
     9961 cgctacgtct tcgtactgcg gaaccgggtt acggaaactc ttggtgacgc aagccaggta
    10021 aatcagaccg atagccgccc agatcagacc cagaaccatt gagctttctt ccaggttaac
    10081 ccatagcgca ccaacggtca gcgcaccaca catcggcagg aacagatact ggaagtgatc
    10141 tttcagcgtc ttgttacgct tctcacggat ccagaactgc gagatgaccg acaggttaac
    10201 aaaggtaaac gccaccagcg caccaaagtt gatcagcgcc gtagccatta ccaggtcgaa
    10261 gttgattgcc agcaaggcaa tcgcgccaac caggatgatg ttcatcgccg gagtacgcca
    10321 tttcgggtgt acataaccga agaagctttt cgggaacacg ccgtcacgac ccattacgta
    10381 catcagacgc gctacgcctg catgcgccgc cataccggac gccagtacgg taatggtgga
    10441 gaagatcagc gcgccaacct ggaacgcttt acccgcaacg tacagcatga tttcaggctg
    10501 tgacgcatca ggatctttaa agcgagagat atccgggaag tacagctgca ggaagtaagt
    10561 tgcaaagatg aagatcatgc cgccaatcag cgcggtcagg aaaatcgcac gcgggatcac
    10621 gcgctctgca tctttggttt cttccgacag gttgctgatg ccgtcaaagc cggtaaagga
    10681 gaagcacagg attgtcgccc cggtaatcat cgggataaca tgcgcatcgc cagaccagaa
    10741 cggacgagtg ctcgccagcg taccagcgcc ttcaccttca aatacgccat aaacaaccat
    10801 gcccagaatc accgcgatca gcactacctg caacacgacg attacagtgt tgaagttcgc
    10861 tacggatttc agactacgca ggttaaaggc ggtcataaag gccaccagcg ccaccacaaa
    10921 catccacgat gggatggaag gcaccagagc ttcaaaatag attttcgcca gcagaatgtt
    10981 gatcatcggc gcgaacagat agtcgagcag agaagaccaa cccaccataa agccgacagt
    11041 cgggctaatg gatttctggg cgtaagtgta tgcagagcca gcagaaggat agcggcgaac
    11101 cagcttcccg tagctcagag ccgtaaacag gatcgcaatc aatgcgaacg cataggctgt
    11161 cggcacatga ccatccgtga ggcctgaaac gataccaaaa gtatcaaaca gcgtcatcgg
    11221 ctgcatatag gcaagaccca tcattacaac cggaactaac gtaagcgttt tacgcaattc
    11281 cacgcgagag gtgtttggag taacgttatg cgacatggtc attctccttt acggtgataa
    11341 ccgtcgcgta agcaaaaaat tgccccattt ttttggattc ctcagcgaca acaactgtcg
    11401 atttttagta aatatctatc cggtacgaag cccggcctct tggtatgaat gattggtttg
    11461 aagcaaaaaa taaccgacgc tgatgaaacg tcggttttta gtcatttttt gacagcggcg
    11521 cattgtgcct aaatgggggg gaa
//
LOCUS       DAOFQD010000148        11473 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.148, whole genome shotgun sequence.
ACCESSION   DAOFQD010000148 DAOFQD010000000
VERSION     DAOFQD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.148"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            58..2055
                     /gene="betT"
                     /locus_tag="QBF46_004074"
     CDS             58..2055
                     /gene="betT"
                     /locus_tag="QBF46_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDQ6676188.1"
                     /translation="MSENDTIQKKSKSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVSFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGERIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLAVLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMHQGAADLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLIGIVAIALLIAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMGDVIQPAMTLVQEELNKQG
                     TISHISDTDDERIRLEVDLGNELNFIYEVRLRGYNSPAFAMAAMDNDEQQAEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2119..2622
                     /locus_tag="QBF46_004075"
     CDS             2119..2622
                     /locus_tag="QBF46_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HDQ6676189.1"
                     /translation="MISRWQWILRQTLKKLWFRATLFAMVAIITALLSILFRSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALIISTLLRWIQHLTSL
                     GRVVGCK"
     gene            complement(3129..4280)
                     /locus_tag="QBF46_004076"
     CDS             complement(3129..4280)
                     /locus_tag="QBF46_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDQ6676190.1"
                     /translation="MTYPDPYSRPAPDRFIRRWLVITGCIAALMLLWQFLPAIEAWFS
                     PHETQERTVTPRGDLAADEKTTIELFEKSRGSVVYITTAQLVRDVWSRNVFSVPRGTG
                     SGFIWDDAGHVVTNFHVIQGASSATVKLADGRDYQAALVGASPAHDIAVLKIGVGFKR
                     PPAVPVGTSADLKVGQKVFAIGNPFGLDWTLTTGIVSALDRTLSGDASGPAIDHLIQT
                     DAAINPGNSGGPLLDSAGRLIGINTAIYSPSGASAGIGFAVPVDTVMRVVPQLIKTGK
                     YIRPALGIEVDEQLNARLQALTGSKGVFVLRVTPGSAAHRAGLVGVEVTAGGIVPGDR
                     VISIDGIAVDDVTTLQARLDDKNVGDVVVLLVERAGKTREMLVELQPGV"
     gene            complement(4300..5262)
                     /locus_tag="QBF46_004077"
     CDS             complement(4300..5262)
                     /locus_tag="QBF46_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HDQ6676191.1"
                     /translation="MISRDLRHAQNVGARHRWLNRLQTALLVLTLLGIAAVAGSLLLG
                     DGGLWLALAAAGFTLLLEPAAASGLTLRLYGARPLHPDEAPDLWAVLRELAARAGLPA
                     VPVPHYVPSGVVNAFATGSKHHAAIALTDGLLRSLTPRELTGVLGHEIAHIANEDLRV
                     MGLADSISRLTHLLALLGQLAIVLSLPALLLGVTEVNWPALLLLAVAPQLALLAQLGL
                     SRVREFDADRLAAELTGDPHGLASALAKIERVSRSWRAWLLPGWGNPEPSWLRTHPAT
                     AERIERLLELAPPPAMPPFPSARFVPEVTVSPRPPRWRTGGLWR"
     gene            complement(5249..5737)
                     /locus_tag="QBF46_004078"
     CDS             complement(5249..5737)
                     /locus_tag="QBF46_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HDQ6676192.1"
                     /translation="MNEKQPTLTKTFRAQWAVMTFYERFEQVIALALSAIIAVIIVVS
                     LLQLISIVFSLLIIDAFNPLDHKVFQAVFGMIMTLLIAMEFKHSIVRVALRRDSIIQV
                     KTVILIGLIALARKFVILDPETGPAKVAALAGATLALGAAYWLLRERDDRTAGESSDD
                     QS"
     gene            complement(5749..5991)
                     /locus_tag="QBF46_004079"
     CDS             complement(5749..5991)
                     /locus_tag="QBF46_004079"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDQ6676193.1"
                     /translation="MLLEAASIEDAQVWAERFRQDVAATALVCGEVSISYSASMGVAP
                     LDVRLNSSEQVMQQADSALYQAKRQGRNQVMVANVN"
     gene            complement(5882..6598)
                     /locus_tag="QBF46_004080"
     CDS             complement(5882..6598)
                     /locus_tag="QBF46_004080"
                     /inference="COORDINATES: protein
                     motif:HMM:TIGR00229.1,HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HDQ6676194.1"
                     /translation="MGFWEWDLATDSFRLSARSLAMFGLTSDEEIGATHAAWQARIHP
                     EDLPVVLAKVRAYQENPSERLECEYRVRHRSGVWIWVLDRGRWLGDTGRQLMVGTLLD
                     ISSRKEMEQRLLRMAITDPLTGLSNRRAFNERLQLEWARLKRSPEIQAALVLCDIDHF
                     KRINDTYGHGCGDEVLKHFASRLREHVRATDMAARIGGGGIRCVVGSGIDRRRPSMGG
                     AISSGRCGDSAGVWRGVDQL"
     gene            complement(6768..8474)
                     /locus_tag="QBF46_004081"
     CDS             complement(6768..8474)
                     /locus_tag="QBF46_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDQ6676195.1"
                     /translation="MQDLLGTTLILLAACSLSVVATAAFRVPALLGYLAVGALLGPSV
                     SGVIAPGEALTFISELGVALLLFMVGLEFSLGHFWLTRKTVLASGSLQMVVVAAPLTL
                     VLIGLGQPAQSAALLGAAAAMSSTALVSRQLADQGELTTRHGRSAIAVLVFQDLASVP
                     LLALLAIWARGDSPKIESVLLEVLGVLVLFAAAAVASRRLLHGLLGWVARQGHEESFV
                     LVSLCVVVAAAAAAHAVGVSAALGAFLAGMVLGESDFRHHMESHLKPFRDVLSGLFFV
                     TIGLQLDTAQIVAAPLAVLGWLLALVPLKMGLNFLALRATRLSALDAWRAGIVLGHGG
                     EFALLLLGMVMQQHLIAATMVQPMLVALVLSMGLAPLLIRHHDRWARAFSRSGALGQP
                     PQAEEGEVAERARSLRDHVIICGADDVGLLLSRTLRLAGIPHLLLESDRQRVEAGRAM
                     GAPVSYGDASRLDTLAAAGLAHARLVVLTFARPQTALRIAQAVLDRRLSLSVVVATER
                     VTDAQLLRNLPNVRLYPLYLAAGLGLAEQVLLMLGINADQVDHRIEELRQALSQSGGD
                     RP"
     gene            complement(8478..8918)
                     /gene="trx-GI"
                     /locus_tag="QBF46_004082"
     CDS             complement(8478..8918)
                     /gene="trx-GI"
                     /locus_tag="QBF46_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance system thioredoxin Trx-GI"
                     /protein_id="HDQ6676196.1"
                     /translation="MKNELHIVCPHCQSINSVPAAKLADRPNCGRCQQPLFTGEPIEL
                     TTATFSRHVERSDLPLLVDFWAPWCGPCKMMAPQFQQAARQLEPGIRLAKVNTEAEPH
                     LAAQFGIRSIPTLALFQGGREIARQAGAMGSQDIVRWASAQVGR"
     gene            complement(8908..9513)
                     /locus_tag="QBF46_004083"
     CDS             complement(8908..9513)
                     /locus_tag="QBF46_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676197.1"
                     /translation="MQPTINRRKEIRKIDFGLLKHLFAVLDHEVANSRQFGEFISLDA
                     PVEADAQDVAEAMVEEEVFEFYQPDDTLRLADIIADSQHPDAATIAEEQELVDRSSEF
                     AFAFDLLKDMPRLWRRIFLLIRVDGLDASSVSEILLIPSKEDTLQRWLMQAEAFIAAH
                     LEEAGVSKNGNDWLQGVDWSALSVTRSEATSPQSKQANHEK"
     gene            complement(9470..10162)
                     /locus_tag="QBF46_004084"
     CDS             complement(9470..10162)
                     /locus_tag="QBF46_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676198.1"
                     /translation="MIKKGQPMKPPVKTSFRGRDVAYLVISIVLVGVGYAAYRGLYFV
                     GENVPFAQEMILVFLGAAATIYLTAVLLNRQTELELRKEGQVLLFQQKHDTYMRVIEK
                     VAEIVERERHDFALIDELRVISHKLAVVGSAPVVEAFRRVLDLLVDGLEDGQLNGNDA
                     EQVMHAVAEVTIAMRSDISSDNDPEALRSAAETIMANSRKIERLDDIEHSKSKPDANA
                     ANNKQKKGDSKD"
     gene            complement(10343..11254)
                     /locus_tag="QBF46_004085"
     CDS             complement(10343..11254)
                     /locus_tag="QBF46_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDQ6676199.1"
                     /translation="MHFLNLQVTQAELLSGLISVSQTVAQIVLVMTVAWLVSAVLSRA
                     ITRISKRTRTSKRSADEVKRIETSLRLIRFVSRLVVWALAVTISLSILGISVAPILTT
                     AGVSGIAVGFAAQSLIKDYFSGLVLLLEGQLRIGDIVDIGGVAGQVEEMTLRYVRLRQ
                     LNGDVVYVPNGAITNLTNKTTDYSMAVIDVGVAYRENLQEALNVMESVARELASETTW
                     STRILAEPEVFGVETLGDSSVILRLRLKVLPGEQWSIRREYLHRIKQRFDELGIEIPY
                     PHLTLYAGQDKDGKAPAFRIEAPAGAQ"
BASE COUNT         2466 a         3359 c         3215 g         2433 t
ORIGIN      
        1 ctttgagatg tagtaccctc tatacagtgc ggattttaat taatggaata aatgattatg
       61 agtgaaaatg atacaatcca aaagaagtct aaaagccaga ttaacaaagc ggtattcttt
      121 acatctgctt tgttaatttt ccttcttgtc gcctttgccg ctgtattccc ggatgtcgcc
      181 gacaaaaatt ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc
      241 cttgcagtgg ccctgattct actgagtgtc tcgttcctgg gactctcacg ctacggtgat
      301 atcaaactgg gtccggacca tgcgcagcct gattttagct atcactcctg gtttgcgatg
      361 cttttctcgg cagggatggg gatcggcctg atgttctttg gcgttgctga acctgtgatg
      421 cattatcttt cacctcctgt cggcactcca gagaccgttg cggcagcgaa ggaagcaatg
      481 cgcctgacgt tcttccactg gggcctgcac gcatgggcaa tctatgccat tgtggcactg
      541 atcctggcct ttttcagtta ccgtcacggt ctgcctttaa cgctgcgttc cgcactttat
      601 cccattattg gcgagcgcat atacggacct gtaggccatg cggttgatat tttcgccgtt
      661 attggcaccg tctttggcgt tgcgacatcg ctggggtacg gtgttttgca ggtgaatgct
      721 ggtttgaatc atcttttcgg ggtgcctatt aatgaaacgg tgcaggtgat tctaatcgtt
      781 gtcatcactg gtttagcgac gatttcggtg gtgtccggcc tggacaaggg aatacgtata
      841 ctgtctgagc tcaacctggg cctggcagtg ctgctgctgg cgctggtcct gtgtctggga
      901 ccaaccgtgc ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttctgaactg
      961 gtgagtaaaa cttttaacct ttatgcgtat gaacctaagt cgagcaactg gctggggggc
     1021 tggacattac tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc
     1081 gcaagagtct cgcgcggtcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc
     1141 gccggcttca cgctcatgtg gatgacggtg tttggtaaca gcgcaatcta tctcattatg
     1201 caccaggggg cggctgatct cgccaatacc gttcagcagg atgtcgcgct ggccctgttt
     1261 aatttcctgg agcatttccc gttttcttcc gtgctgtcat ttattgcaat ggcgatggtc
     1321 attgtcttct ttgtcacctc ggctgactcc ggggcaatgg ttgttgatac gctggcatcg
     1381 ggtggcgtcg ctaacacgcc tgtatggcag cgaattttct gggcctcgct catagggatt
     1441 gtggcaatcg cactgctgat tgcgggggga ctgagcgcgc tgcaaaccgt gacaatagcg
     1501 agcgcattac ctttctcagt gattttactg atatccatat acggattatt aaaagccctg
     1561 cgtcgggatt tgaccaagcg tgaaagcctg agcatggcga ctattgctcc gactgcagcg
     1621 cgtaacccga ttccatggca gagacgcttg cgcaatatcg cttatctgcc aaagcgctct
     1681 cttgtgaaac gctttatggg cgacgttatc cagcccgcca tgacgctggt tcaggaagaa
     1741 ctgaataaac agggaacgat aagccatata agtgatactg atgacgaacg aattcggctt
     1801 gaagtcgatt tgggcaatga gctgaacttt atttatgaag tgagacttcg tggctataac
     1861 tcaccggctt tcgccatggc tgcaatggac aatgacgagc agcaggctga gcaacatcga
     1921 tattatcgcg ctgaggtcta tctgaaagaa ggcggtcaaa actatgatgt gatgggctgg
     1981 aatcaggaac agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg
     2041 cacctcgttc gttaatacaa aaatgccgtc ctgccgggcg gcatttattt tcccgaactt
     2101 aatacaagga tatgctgaat gatttcacgc tggcaatgga ttctcaggca gacacttaaa
     2161 aagctatggt tcagggcaac gttatttgca atggtagcaa taataacggc ccttttatct
     2221 attcttttta gatcaatgat cccagagtca gtatcagtga aggttggtgc ggaagcagtt
     2281 gataatattc tgaacatact ggcatcgagt atgctggcgg tgaccacatt ttcactgagt
     2341 atcatggtca ccgcttacgg ttcagccact actaatgtga cgcccagagc cacgcgttta
     2401 gttgtagaag acgtaaccac acaaaatgta ctggccactt ttatcggttc ttttctcttc
     2461 agcctggtag ggattattgc cctcaatatg ggagcctacg gagaaagggg tagagtcatt
     2521 ttattcattg taacactggt tgtcattgcc ttaataatca gcacattgct tcgctggata
     2581 cagcatttga cctctctggg cagggttgtg ggatgcaaat aaaatttcat cctaacccta
     2641 aaaacagaga gaaacgtcgc gcgctctcct attcataaaa aatccatttt atatcagtat
     2701 gttgtgagca cttacacccg agactggatc gttttgcctt tcatgggaga atgattaagg
     2761 gcgctaaagt atcatcctaa ttttcgccca ggctcgaaaa atacggcaaa tggtgaaaat
     2821 aaagattcat ccttttccga tgttgcccgt tccaacacgt aagcagtcag tcagaaaaaa
     2881 cacatagatc caatccctag cgagcattga gctctctcat ccgtgccaac tgctccaacc
     2941 cttggacgcc ctaaatgctt acccaaaaaa atcaacacct tcttttaaaa acacaatcaa
     3001 aaatgactcc cttatatcca tttaacctaa acagccttcc ccccactgca ctagctaggg
     3061 ctcgtagttc ggcgcaaggc tgaagtcgct tggctgcttc gctgtgcaga tccatgaccc
     3121 actttccatc aaactcccgg ttgtagttcc acaagcatct cgcgagtctt gccggcccgc
     3181 tccactaaca agaccacaac atctccaacg ttcttgtcgt ctagccgggc ctgtaatgtg
     3241 gtgacgtcgt cgacggcgat accgtcaatg ctgataacgc gatcgccggg cacgatgccg
     3301 cctgcggtga cctcgacgcc gacgagcccg gccctgtgcg ccgccgagcc cggcgtcacg
     3361 cgcaatacga atacgccctt actgccggtc agcgcctgca gacgcgcgtt gagctgctca
     3421 tccacctcga tgcccagcgc cggacggatg tacttgccgg tctttatgag ttgcggcacc
     3481 acgcgcatga cggtatcgac cggcaccgca aagccgatgc cggccgaggc gccagacgga
     3541 ctgtagatgg cggtattgat gccgatcagc cgcccagccg aatcgagcag cgggccaccg
     3601 gaattgccgg ggttgatagc ggcgtcggtc tggatcaggt ggtcaatggc cgggccactg
     3661 gcgtcgccgg aaagggtgcg gtcaagcgcc gagacgatgc cggtggtgag cgtccagtcg
     3721 agcccgaagg gattgccaat ggcaaagacc ttttgcccca ccttgagatc ggcactggtg
     3781 cccaccggca ccgccggcgg gcgcttgaag ccgacgccaa tcttgagtac cgcgatgtcg
     3841 tgcgcaggac tggcgccaac gagcgcagcc tgataatcgc gaccgtcggc cagtttgacc
     3901 gtggcagacg atgccccctg gatcacgtgg aagttggtca ccacgtggcc ggcatcgtcc
     3961 cagatgaagc cggagccggt gccgcgcggc acggaaaaga cattgcgcga ccagacgtca
     4021 cgcactagtt gtgccgtggt gatgtaaacc accgacccgc gcgatttctc gaacagctcg
     4081 atggtggttt tttcgtcggc ggccaggtcg ccgcgcggcg tcaccgtgcg ctcctgcgtt
     4141 tcgtggggac tgaaccaggc ttcgatggcg ggcaggaact gccacagcag catgagtgcg
     4201 gcgatgcagc cagtgatgac gagccagcgc cggatgaatc gatccggcgc cgggcggctg
     4261 taggggtcgg ggtaggtcac gtcattctcc atgttgaaat cagcgccaaa ggccgccggt
     4321 gcgccagcgt ggcggacgtg gtgatacggt cacctcgggg acgaaacggg ccgatggaaa
     4381 cggcggcatc gcgggcggcg gagcaagttc cagcaagcgc tcaatgcgtt cagccgtcgc
     4441 cggatgcgtg cgcaaccagg agggttccgg attgccccat ccgggcagca gccaggcgcg
     4501 ccaggagcgg ctcacccgct cgatcttggc gagcgccgag gccagcccgt gcgggtcgcc
     4561 ggtcaattcg gcagcgagcc ggtcggcgtc gaattcgcgc accctggaca agcccaactg
     4621 agccagcaag gccagctgtg gcgcgaccgc cagtagaagc aacgcgggcc aattgacttc
     4681 cgtgactcca agcaatagcg ctggcaagct gagcacgatc gcaagctgcc ccaacaaggc
     4741 cagaagatgg gtgagccggc tgatcgaatc ggccaagccc atgacacgca aatcctcgtt
     4801 cgcaatatgc gcgatttcgt ggccgagcac gccggtcaac tcgcggggcg tgaggctacg
     4861 cagcaggccg tcggtcagcg cgatggccgc gtgatgcttc gaaccggtgg cgaaggcgtt
     4921 gacgaccccg ctgggcacgt agtgcggtac cggcacggca ggcagcccgg cccgcgccgc
     4981 cagttcgcgc aacacagccc aaagatcggg cgcttcatcc gggtgcaggg ggcgtgcgcc
     5041 atacagccgc aaggtcagcc cggaggcggc cgccggttcg agcagcaggg tgaaccccgc
     5101 ggccgcaagc gccagccaaa gcccgccgtc acccaacagc aggctgccag cgacggcggc
     5161 aatcccgagc agggtcagca ccaacagcgc ggtttgcagg cgattgagcc agcgatgccg
     5221 cgcgccgaca ttctgcgcgt ggcgcagatc acgactgatc atctgagctt tccccggctg
     5281 tccggtcatc gcgctcgcgc agcagccagt aagccgctcc gagggccagc gtcgcgcctg
     5341 cgagcgccgc gaccttggcg gggccggtct ccgggtcgag gatcacgaat ttacgcgcca
     5401 gggcgatcag cccgatcagg atcaccgtct tgacctggat gatgctgtcg cgccgcaagg
     5461 ccacccgcac gatggaatgc ttgaactcca tggcgatgag cagcgtcatg atcatgccga
     5521 acacggcctg gaagaccttg tgatcgagcg gattgaacgc atcaatgatc agcaggctga
     5581 agacgatcga gatcagctgc agcagcgaga ccacgatgat cacggcaatg attgcggaca
     5641 gcgcgagcgc gatcacctgt tcgaagcgct cgtagaacgt catcactgcc cattgggcgc
     5701 gaaatgtttt cgtcagcgta ggttgctttt cattcatcgc catttccctc aatttacatt
     5761 ggcaaccata acttggttgc gcccttgtct ttttgcctgg tagagcgcgc tgtccgcctg
     5821 ctgcatcacc tgctcggaag agtttaagcg gacgtcgagg ggagccaccc ccatgctggc
     5881 gctatagctg atcgacacct ctccacacac cagcgctgtc gccgcaacgt cctgacgaaa
     5941 tcgctccgcc catacttggg cgtcttctat cgatgccgct tccaacaaca cagcgaattc
     6001 ctcccccccc aatccgtgca gccatgtcgg ttgcgcgaac atgttccctc aaccgtgaag
     6061 caaagtgctt gagcacctca tcgccgcatc cgtgcccata ggtatcattg atgcgcttga
     6121 agtgatcgat atcgcagagc acaagtgctg cttgtatctc cgggctgcgt ttgaggcgcg
     6181 cccattccag ctgtaatcgt tcgttaaaag cccgccggtt cgacaagccg gtcaaggggt
     6241 cggtgatcgc catgcgcagc aaccgctgct ccatttcctt gcgagaagag atgtcgagca
     6301 gagtgcccac catcaattgc ctgcccgtgt cacccagcca acgaccgcga tccagcaccc
     6361 aaatccaaac gccggatcga tgacgaacgc ggtactcgca ctccaatcgc tccgacggat
     6421 tttcttggta agcgcgcacc tttgccagaa cgaccggtaa atcctccgga tgaatacgcg
     6481 cttgccatgc ggcatgggtg gcgccaatct cctcgtcgga ggtcaagccg aacatcgcca
     6541 gactgcgggc agaaagcctg aagctgtcgg tggcaagatc ccactcccag aatcccaact
     6601 ctgcgccttc cagggcgatg gcgatccgtt cgttgtcgtc gattttcgct tgttggcgat
     6661 gcttcagctc gtcacgcctg tggcgattgg tacgagcaaa acgccataac gcaaccccat
     6721 gccaacattg gcaagtagcg acaccatcag ccagatggtc ggcccgttca tggtctatcc
     6781 ccgcctgact gactgagtgc ctgccgtaat tcctcgatcc ggtggtcgac ttggtcggcg
     6841 ttgatgccca acatcaacag tacctgctcg gccagtccca gtcccgcagc caggtacaac
     6901 gggtacagtc tgacattggg cagattacgc aaaagctggg catccgttac gcgctcagtg
     6961 gccaccacca cggacaagct caggcggcgg tcgagaaccg cctgagcgat acgcagggcc
     7021 gtctgcggac gggcaaacgt cagcaccacc aggcgggcat gggccaatcc ggcggcggcc
     7081 agggtgtcga gccgactggc atcgccatac gacaccggcg cccccatagc acgccccgct
     7141 tcgacccgtt ggcggtccga ttccagcagc aaatgcggta tgcctgccag gcgcagcgtt
     7201 cggctcagca gcagcccgac gtcatccgcc ccacagatga tcacatggtc gcgcaacgac
     7261 cgcgcccgct cggcgacttc gccttcttcg gcctgcggtg gctgaccgag tgcgcccgag
     7321 cggctgaaag cccgcgccca tcggtcatgg tgtcggatca ggagcggtgc caaccccatg
     7381 ctcaacacca gcgccaccag catcggttgc accatggtgg cggcgatcag gtgctgctgc
     7441 atgaccatgc ccaggagcag cagcgcaaac tcgccgccat gacccagcac gatgcctgcg
     7501 cgccaggcgt cgagcgccga caggcgggtt gcgcgcagcg ccagaaagtt cagccccatc
     7561 ttgagaggca ccaaggccaa cagccaaccc agtaccgcca gcggtgcggc cacgatctgc
     7621 gcggtgtcca gctgcaggcc aatggtcacg aagaacaggc ccgacagcac atcacgaaag
     7681 ggcttgaggt ggctttccat gtggtgccgg aagtcgctct cgcccaggac catgccggcg
     7741 agaaaggccc ccagggcggc ggatacgccg acggcgtgtg cagcagcggc tgccgccacg
     7801 accacgcaca aggagaccag cacgaaggat tcttcgtggc cctgccgcgc cacccagccc
     7861 agcaggccat gcaacagacg acgcgaggca accgccgctg ccgcgaacaa caccagcacg
     7921 cccaacactt cgagcagcac gctctcgatc ttcggcgagt cgccgcgcgc ccagatcgcc
     7981 agcagggcca gcaggggcac gctggccagg tcctgaaaga ccagcacggc gatggcactg
     8041 cggccgtggc gggtggtgag ctcgccttgg tcggccagct gtcggctgac cagcgccgtg
     8101 gacgacatgg ccgctgcggc gccgagcagt gcagcgctct gagctggctg acccaacccg
     8161 atcagcacca gggtcagtgg cgcggccacg acgaccatct gcaagctgcc ggacgccagt
     8221 acggtcttgc gagtgagcca gaagtgcccc agcgagaatt ccagtccgac catgaacagc
     8281 agcagggcca cacccagttc ggatatgaaa gtcaacgcct ctcccggcgc gatcacaccg
     8341 ctgaccgagg ggcccagcaa ggcgccgacg gcgaggtaac ccagcaaggc gggtactctg
     8401 aacgccgccg tcgccaccac cgagaggctg cacgccgcca gcaggatcag ggtagtgccg
     8461 agcaaatcct gcatcggtca gcgtcccacc tgtgcagagg cccagcgcac gatgtcctgc
     8521 gagcccatgg cgcctgcctg gcgggcgatc tcacgtccgc cctggaacag ggcgagcgtc
     8581 ggaatgctgc ggataccgaa ctgcgcggcc aggtggggct cagcctcggt gttcaccttt
     8641 gccagcctga tcccgggttc gagctggcgc gcggcttgtt gaaactgcgg cgccatcatc
     8701 ttgcacggcc cgcaccaggg tgcccagaaa tcgaccagca gcggcagatc gctgcgctcc
     8761 acgtggcgcg agaacgtcgc cgtcgtcaac tcgatgggct cgccggtgaa caagggctgc
     8821 tggcagcggc cgcagttggg acgatccgcg agcttggcgg ccggcacgct gttgatggac
     8881 tggcaatgtg ggcagacgat gtggagttca tttttcatgg ttcgcttgct tggactgcgg
     8941 tgaggttgcc tcgcttcggg tcacagacaa tgccgaccaa tcaacgcctt ggagccaatc
     9001 gttcccgttt ttgcttactc cggcctcttc cagatgagca gcgatgaatg cttcggcctg
     9061 catgagccac ctttggagag tatcttcctt actaggaatc agcaggatct cagagacgct
     9121 gctggcatcc agcccgtcca cacggataag cagaaaaatg cgccgccaca aacgcggcat
     9181 gtccttcagc aggtcgaatg caaaagcgaa ctcgctcgac cgatcaacta gctcctgctc
     9241 ttccgcgatc gttgcggcat cgggatgctg actgtcagcg atgatatcgg ccagcctgag
     9301 tgtgtcatcg ggctgataaa attcgaagac ttcctcttcc accatggcct cggcgacgtc
     9361 ctgggcatcc gcttcgaccg gcgcgtccag agaaatgaat tcgccaaatt gccggctgtt
     9421 tgccacttcg tgatccagga cggcgaacag atgtttcaga agaccgaagt caatctttcg
     9481 aatctccttt cttctgttta ttgttggctg catttgcgtc tggcttggac tttgagtgct
     9541 cgatgtcgtc cagccgctcg atcttgcgtg agttcgccat gatcgtttct gcagccgacc
     9601 gaagcgcttc agggtcgttg tcgctgctga tgtccgaacg catcgcaatc gtgacttcag
     9661 ctacggcatg catcacctgt tctgcgtcat tgccgttcaa ctggccatcc tcaagtccat
     9721 caacaagcag atcaagaacg cgtcggaacg cttcgacaac tggcgcgctg ccgacaacgg
     9781 caagcttgtg gctgatgacc ctgagttcat caatgagcgc aaaatcatga cgttcacgct
     9841 ccacgatttc agcaaccttt tcgatgacgc gcatatatgt gtcatgcttt tgttgaaaaa
     9901 gaagaacttg cccttctttt cttaactcaa gctcagtctg gcgattgagg agcacagctg
     9961 tcagatagat agtggcagct gcacccagga acactaggat catctcttgg gcaaaaggaa
    10021 cgttttcacc aacaaagtat aagccgcggt aggctgcgta acctacgccc actaaaacaa
    10081 tggaaattac caggtaggcg acgtccctgc cgcgaaagct cgtctttacc ggtggcttca
    10141 tgggttggcc tttcttaatc atggctgtcg ttgcctagcg aacgggtgtc aggggcaggt
    10201 tcggtctcct tttcgggccg caagagaata ggggttggtc cagagccaaa tacattgaac
    10261 ccatcccgca gacagcgctc gaaaatcgtt tcatcccagt tcgagaaatc tctttccact
    10321 tcatatgtat attttggccg tgtcattggg caccagccgg agcttcgatc cgaaaggcag
    10381 gggcttttcc atccttgtcc tggccggcat aaagcgtcag atgcggataa gggatttcga
    10441 tgccaagttc gtcgaagcgc tgttttatcc tgtgcagata ttcgcgccgg attgaccatt
    10501 gctcacctgg caagaccttg aggcgaagac gcaggataac cgaagagtca cccaacgttt
    10561 cgaccccaaa gacctctggc tcggctaata ttctcgttga ccaggttgtt tccgaagcta
    10621 gctcacgggc gacactctcc ataacgttca atgcctcctg aaggttttcg cgataggcga
    10681 caccgacatc gattacggcc atcgaatagt ccgttgtctt atttgtcaga ttggtgatgg
    10741 ctccgttggg cacatagacc acgtcaccat tgagttgacg caggcgcaca tatcgcaacg
    10801 tcatctcttc gacttggcct gccacaccgc caatatccac gatatccccg atgcggagtt
    10861 gcccttccag caacaagacc aaacccgaaa aatagtcttt tatcaggctc tgcgcagcaa
    10921 accccactgc gatgccgctt acccccgcag tcgtcaggat cggtgccaca gaaatgccaa
    10981 ggattgagag ggaaatggtc acggccaagg cccacacgac cagccgactg acgaaacgga
    11041 tcagacgaag agatgtttca atgcgtttga cctcatctgc agatctcttg gaagtgcggg
    11101 ttcttttgct gatgcgtgtg atggcccgcg aaagcactgc ggacacgagc cacgccacgg
    11161 tcatgacgag cacaatctgt gcaactgttt gggaaacgga aataaggccg gataggagct
    11221 ctgcctgtgt gacctgcaaa ttcaaaaaat gcatttatat gcactcccta ttattctggg
    11281 gtaagcggat taaatggttg atcttacctt tgcatgagag tgagacagac cggcatctca
    11341 tcggattatc tggttgatgc ttggcggcgg caaaaactga gtgcaacacc tgacctttcc
    11401 ggtacggtgc tgcccccatg tcttataccg aaccgctttt tcttccggca cggattgcca
    11461 ttccacctca gct
//
LOCUS       DAOFQD010000149        11463 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.149, whole genome shotgun sequence.
ACCESSION   DAOFQD010000149 DAOFQD010000000
VERSION     DAOFQD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.149"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1380
                     /locus_tag="QBF46_004086"
     CDS             <1..1380
                     /locus_tag="QBF46_004086"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDQ6676200.1"
                     /translation="FADIQPMTDEFGWSRRNAFIQPQVDAVMLEGLSRFPNVRCLFSR
                     ELEAFSQQNGEVTLNLKAPDGQRETVKAQWLVACDGGGSNVRRSLNVPFEGKIAPNQW
                     IVVDIANDPLSTPHVYLCCDPVRPYVSAALPHAVRRFEFMVMPGETEAQLSEPQNMRQ
                     LLSKVLPNPDNVELIRQRVYTHNARLAERFRIDRVLLAGDAAHIMPVWQGQGYNSGMR
                     DAFNLAWKLALVINGKADDALLDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWHGAVR
                     DGVSWLLNYIPPVKRYFLEMRFKPMPQYHAGALVREGDAKTSPVGKMFIQPKVTLESG
                     EVTLLDNVIGPNFAVIGWGCNPLWGMSEAQIQQWQALGTRFIQVVPDTQIHTDQDNHD
                     GVTRIGDTQNRLRAWFAQHNAALVVMRPDRFVAAIAIPQTLGSTLNKLASVMTLTRAT
                     ADIPVEKVA"
     gene            1382..2335
                     /gene="mhpB"
                     /locus_tag="QBF46_004087"
     CDS             1382..2335
                     /gene="mhpB"
                     /locus_tag="QBF46_004087"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDQ6676201.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQAVLDEVNGVIASARARIAAFEPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELLVPTDLAEACAHAVMKSG
                     IDLAVSYNMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FASSLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKQLPENERELRQQRVIS
                     AAEKFVVDQNTLHPLNPVWDNRFMSLLEQGRLQGLDAVSNEELSAMAGKSTHEVKTWV
                     AAFAAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSATTQN"
     gene            2364..3230
                     /locus_tag="QBF46_004088"
     CDS             2364..3230
                     /locus_tag="QBF46_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDQ6676202.1"
                     /translation="MTYQPQTEAATSRFLEVQEAGETLRVHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVQAGYRVILLDCPGWGKSDGIVNRGSRSDLNARILKSVVDQ
                     LDIQTVHLLGNSMGGHSAVAFTLSWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYREPTIENLKLMMEIFVFDTSDLTEALFKARLNNMLSRRDHLENFVQSLEANPKQFP
                     DFSPRLGEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIYRDCGHWAQWEHADA
                     FNQLVLNFLTRP"
     gene            3240..4049
                     /gene="mhpD"
                     /locus_tag="QBF46_004089"
     CDS             3240..4049
                     /gene="mhpD"
                     /locus_tag="QBF46_004089"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDQ6676203.1"
                     /translation="MTNRNLEQLAADLRQAEEKGEAIAPLRELIGVDNAEAAYAIQRI
                     NVQYDVARGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPNTDTTFDELYNAIEWVLPALEVVGSRIRDWSIKFVDTVADNAS
                     CGVYVVGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPINAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIDGIGSVAATFSAAVTKGSQS"
     gene            4046..4996
                     /locus_tag="QBF46_004090"
     CDS             4046..4996
                     /locus_tag="QBF46_004090"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDQ6676204.1"
                     /translation="MSKRKAAIIGSGNIGTDLMIKILRHGVHLEMAVMVGIDPESDGL
                     ARARRMDVATTHEGVTGLIAMPEFADIDIVFDATSAGAHVKNDAALRAVKPGIRLIDL
                     TPAAIGPYCVPVVNLAANVDQQNVNMVTCGGQATIPMVAAVSRVAKVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSKAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSEAAS
                     EVEVEASINEMAVAVQAYVPGYRLKQRVQFEVIPEDKPVNLPGIGQFSGLKTAVYLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARQAGEAA"
     gene            4993..6006
                     /gene="dmpG"
                     /locus_tag="QBF46_004091"
     CDS             4993..6006
                     /gene="dmpG"
                     /locus_tag="QBF46_004091"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDQ6676205.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAYQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAQQAKLMENYGA
                     TCIYVVDSGGAMNMSDIRARFRALKAVLKPETETGMHAHHNLSLGVANSIAAVEEGCD
                     RVDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCEVAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NRK"
     gene            6076..7287
                     /gene="mhpT"
                     /locus_tag="QBF46_004092"
     CDS             6076..7287
                     /gene="mhpT"
                     /locus_tag="QBF46_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDQ6676206.1"
                     /translation="MTTRTSASYRLTLTVGLCFLVALMEGLDLQAAGIAAVGIAHAFA
                     LDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIASVALFGLFSLATAISWDFS
                     SLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAAALGFSG
                     LASAWQTVFWVGGVIPLLLVPLLMRWLPESQVYTQQTQVAAAPLRALFAPATASATLL
                     LWLCYFFTLLVVYMLINWLPMLLVEQGFRPSQAAGVMFALQIGAASGTLILGALMDKL
                     RPVVMSLLIYTGMLASLLALGTVSSLSGMLMAGFVAGMFATGGQSVLYALAPLFYSTQ
                     IRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILLAGLAVFILMSR
                     KSQMPIPASGA"
     gene            7562..8965
                     /gene="mmuP"
                     /locus_tag="QBF46_004093"
     CDS             7562..8965
                     /gene="mmuP"
                     /locus_tag="QBF46_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HDQ6676207.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            8952..9884
                     /gene="mmuM"
                     /locus_tag="QBF46_004094"
     CDS             8952..9884
                     /gene="mmuM"
                     /locus_tag="QBF46_004094"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HDQ6676208.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIRKVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVAFLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
     gene            complement(9993..11039)
                     /gene="fbpC"
                     /locus_tag="QBF46_004095"
     CDS             complement(9993..11039)
                     /gene="fbpC"
                     /locus_tag="QBF46_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDQ6676209.1"
                     /translation="MTQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVPRAELKARVKEALAMVDLEGFEDRFVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFDITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPATFSDGYVDIYGYHLPRPLHFGTQGEGMVGVR
                     PEAITLSDRGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDVGEQYYL
                     EIHPYGMFVLADAA"
     gene            complement(11051..11398)
                     /locus_tag="QBF46_004096"
                     /pseudo
     CDS             complement(11051..11398)
                     /locus_tag="QBF46_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         2269 a         3082 c         3553 g         2559 t
ORIGIN      
        1 tttgctgata tccagccgat gaccgacgaa tttggctggt cgcgtcgtaa tgcgtttatt
       61 cagccgcagg tggacgcggt gatgttggaa gggttgtcgc gttttcccaa cgtgcgctgc
      121 ctgttctccc gcgagctgga ggccttcagc caacaaaacg gcgaagtgac gctaaatctg
      181 aaagcgccag acggtcaacg tgaaacggtg aaggctcagt ggctggtggc ctgcgacggc
      241 ggcggcagca acgtcagacg ttctctgaac gtgccgtttg aaggtaaaat cgcgccgaat
      301 cagtggattg tggtggatat cgccaacgat ccgctcagca cgccgcatgt gtatttatgc
      361 tgcgatccgg tgcgcccgta tgtctctgcc gcgctgccgc acgcggtgcg ccgttttgag
      421 tttatggtta tgccggggga aaccgaagcg cagcttagcg agccgcagaa tatgcgccag
      481 ttactgagca aagtgctgcc gaacccggac aacgtcgagc tgatccgcca gcgcgtgtat
      541 acccataatg ctcgtctggc cgaacgtttt cgtattgacc gcgtgctgct ggcgggcgat
      601 gcggcgcaca ttatgccggt ctggcagggt cagggataca acagcggcat gcgcgatgct
      661 ttcaacctgg catggaaact ggcgctggtg attaacggta aagccgacga tgcgctgttg
      721 gatacctacc agcaggaacg ccgcgaccac gccaaagcga tgatcgacct ctccgtgacc
      781 gctggcaacg tacttgcgcc gcccaaacgc tggcacggcg cggtacgcga tggggtttcc
      841 tggctgttga actatattcc gccggtcaaa cgctacttcc ttgaaatgcg gtttaagccg
      901 atgccgcagt accacgccgg ggcgctggtg cgtgaaggcg acgcgaaaac ttcaccggtc
      961 ggcaagatgt ttatccagcc gaaggtgacg ctggaatcgg gcgaagtgac gttgctcgac
     1021 aacgtcatcg gaccgaattt tgcggtgatt ggctggggct gtaacccact atggggcatg
     1081 agcgaggcgc agatccaaca gtggcaggcg ctggggacgc gttttatcca ggtcgtgccg
     1141 gatacgcaga tacataccga tcaggataac cacgacggcg ttacccgcat aggcgatacg
     1201 caaaaccgcc tgcgcgcgtg gtttgcccag cacaacgccg cgctggtagt catgcgcccg
     1261 gaccgcttcg tggccgccat cgccattccg caaaccctgg gcagcaccct gaataaactg
     1321 gcgtcggtga tgacgctcac tcgcgctact gccgacattc ctgttgaaaa ggttgcctga
     1381 tatgcacgct tatcttcact gtctttccca ctcgccgctg gtgggctatg tcgacccggc
     1441 acaggctgtg ctcgacgagg ttaatggcgt gatcgccagc gctcgggcac gtattgcggc
     1501 gttcgaacct gaactggtcg tgctgtttgc ccccgatcac tacaacggat ttttctacga
     1561 cgtgatgccg ccgttctgtt taggcgttgg cgctacggcg atcggcgatt ttggtagcgc
     1621 ggcgggggaa ttactggtgc cgacagatct ggctgaagcc tgcgcccacg cggtaatgaa
     1681 aagcggtatc gatctcgccg tgtcctacaa catgcaggtc gatcatggct tcgcgcagcc
     1741 gctggagttt ttgctcggcg gcctggataa ggtgccggtg ctgccagtgt ttattaacgg
     1801 cgtcgcaacg ccgctgccgg gctttcagcg tacccgcatg ctcggtgaag cgattggccg
     1861 tttcgccagc tcgctgaata agcgcgtgct gtttttaggc tccggcgggc tatcgcatca
     1921 gccgccggta ccggagctgg cgaaggctga tgcccatatg cgtgaccgcc tgctcggcag
     1981 tggcaaacag ttacctgaaa acgagcgcga gctgcgccag cagcgggtga tcagcgccgc
     2041 cgagaagttt gtggtcgatc aaaacacgct acacccgctt aaccctgtct gggataaccg
     2101 ttttatgtcc ctgcttgagc aggggcgttt gcaggggctg gacgcggtca gtaacgaaga
     2161 gctgtccgcg atggcgggca aatcaacgca cgaagtgaaa acctgggttg ccgcctttgc
     2221 cgcctttgcc gccatttctg cttttggcaa ctggcgcagc gaaggccgtt attaccgccc
     2281 gatcccggag tggatcgccg gattcggctc cctgagcgcc actacacaga actaaaacag
     2341 aactgaatat gcaggagaac atcatgactt accaaccgca aaccgaagcc gctaccagtc
     2401 gttttctgga agtgcaggaa gcaggcgaaa ccctacgcgt gcatttcaat gactgtgggc
     2461 agggcgatga gaccgtcgtg ctgttgcatg gctctggtcc tggcgcgacg ggctgggcaa
     2521 acttcagccg taatatcgat ccgttggtac aggccggata ccgggtgatc ctgctggatt
     2581 gtcctggctg gggcaaaagc gatgggatcg ttaaccgcgg ttcgcgatcc gatctcaacg
     2641 cccgcatcct gaaaagcgtg gtcgatcagt tggatattca aacagtccac ctgctgggta
     2701 actcgatggg cggtcacagt gctgtggcgt ttaccctgag ctggccggag cgcgtgggca
     2761 aactggtatt aatgggcggc ggaacgggcg gcatgagcct gtttaccccg atgccgaccg
     2821 aaggtatcaa gcgcctgaat cagctgtatc gtgagccgac catcgagaac ctgaagctga
     2881 tgatggagat ctttgtcttc gacaccagcg acctgaccga ggcgttgttt aaggcgcgcc
     2941 tgaataacat gctgtcgcgt cgcgatcacc tggaaaactt tgtccagagt ctggaggcca
     3001 acccgaaaca gttcccggat ttcagcccgc gtctgggtga aatcaaagcc cagaccctga
     3061 ttgtctgggg acgcaatgac cgctttgtgc cgatggatgc cggtctgcgc ctgctttctg
     3121 ggattgccgg ttctgaactg cacatctacc gcgactgcgg gcactgggcg cagtgggaac
     3181 atgcggatgc ctttaaccaa ctggtgctga atttcctcac acgcccttaa ggaacggaca
     3241 tgacgaatcg taatcttgag cagctagccg ccgatttacg tcaggctgaa gaaaaagggg
     3301 aagccatcgc cccactgcgt gaattgattg gtgtggataa cgccgaagcg gcctacgcca
     3361 ttcagcgcat caacgtgcag tacgacgtgg cgcgcggccg tcgcgtggtc gggcgcaaag
     3421 tggggctgac gcacccgaaa gtgcagcaac agctgggtgt cgatcaaccg gacttcggta
     3481 cgctgtttgc tgatatgtgt tatggcgata acgaaaccat cccgttttcc cgtgtgctgc
     3541 aaccgcgtat tgaagccgaa attgcgctgg tgctgaaccg cgatctgccc aataccgaca
     3601 ccacttttga cgagctttac aacgccatag agtgggtact tcccgcgctg gaagtggtcg
     3661 gcagccgtat ccgcgactgg tcgatcaaat ttgttgatac cgtggcggat aacgcctcct
     3721 gtggcgtgta tgtggtcggc ggtccggcac agcgtccggc aggattagat ctgaaaaact
     3781 gcgcgatgaa aatgacgcgc aacaacgaag aggtctccag cgggcgcggc agcgaatgcc
     3841 tgggacatcc gatcaacgcc gccgtctggc tggcgcgcaa aatggccagc ctcggcgagc
     3901 cgctgcgtgc cggagacatt attctcaccg gagcgttagg tccgatggtg gcggtgaatg
     3961 cgggcgatcg tttcgaagcc catatcgacg gtattggttc agtcgccgcc accttttcag
     4021 cggcggttac aaaaggaagt cagtcatgag taagcgtaaa gccgccatta tcggctccgg
     4081 caacatcggt accgatttga tgataaaaat tctgcgtcac ggcgtgcatc tggaaatggc
     4141 tgtgatggtt ggtatcgatc cagaatctga tggcctggcg cgtgctcgac gcatggacgt
     4201 tgccaccacc catgaagggg tgacagggct tatcgccatg ccggaattcg ccgatatcga
     4261 catcgtgttt gacgccacca gtgcaggcgc gcatgtgaag aacgacgccg cgctacgtgc
     4321 cgttaaacct ggcatccgcc tgatcgacct gacgcccgcg gcaatcggtc cttactgcgt
     4381 gccggtggtg aatctggcag ccaacgttga tcagcagaac gtcaatatgg tgacctgcgg
     4441 cggacaggcg accatcccaa tggtggcggc agtttctcgc gtggcaaaag tccattacgc
     4501 cgagattatc gcctcgatcg ccagtaaatc ggcggggccg ggtacccgcg ccaatatcga
     4561 cgagtttacc gaaactacct cgaaggcgat tgaagtggtc ggcggcgcgg caaaaggcaa
     4621 agccattatt gtccttaacc cggccgaacc gccgctgatg atgcgtgaca cggtgtatgt
     4681 gctgagcgaa gcggcctctg aggttgaagt tgaagcgtcg attaatgaga tggcggtggc
     4741 ggtacaggcc tacgtgccgg gctatcgcct gaaacagcgt gtccagtttg aagtcatccc
     4801 ggaagataaa ccggtgaatc tgccgggtat cggccagttc tctgggctga aaaccgcggt
     4861 gtatctggaa gtggaaggcg cggcgcacta tttacccgcc tacgcgggca acctcgacat
     4921 tatgacctcc agcgcgctgg cgaccgctga gaagatggcg cagtccctcg ctcgtcaggc
     4981 aggagaagca gcatgaacgg taaaaaactc tatatctctg acgtgaccct gcgcgacggt
     5041 atgcacgcta ttcgtcatca atattcgctg gagaatgtgc gccagatagc caaagcgctg
     5101 gacgacgcac gggttgattc tattgaagtg gcccacggcg acggtttaca aggttccagc
     5161 ttcaactacg gctttggtgc acacagcgac ctggagtgga ttgaagcggc ggcggacgtg
     5221 gtgaagcatg ccaaaattgc cacgctgttg ctgccgggta tcggcactat tcatgacctg
     5281 aaaaatgcat atcaggccgg cgcacgggta gtgcgcgtgg cgacccactg cactgaagcg
     5341 gatgtgtcgg cccagcatat tcagtatgcg cgtgagcttg gcatggatac cgtcggtttt
     5401 ctgatgatga gccatatgac cacaccggag aacctggcac agcaggcgaa gctgatggaa
     5461 aactacggcg cgacctgtat ttatgtggtc gattccggcg gcgcaatgaa catgagcgat
     5521 attcgcgccc gcttccgcgc actgaaagcg gtgctgaagc ctgaaacaga aaccggaatg
     5581 catgcgcatc acaacctgag tcttggggtg gcgaactcga ttgccgcagt agaagagggg
     5641 tgtgaccgtg tcgacgccag cctggcaggg atgggcgcag gcgcgggcaa cgcaccattg
     5701 gaggtattta tcgccgctgc ggataagctt ggttggcagc acggaaccga tctctacgca
     5761 ctgatggacg ccgccgacga tttagtgcgt ccactacaag accgcccggt gcgggtggat
     5821 cgtgaaacgc tggcgctggg ctatgccggg gtgtactcca gttttctgcg tcattgcgaa
     5881 gtggcggcag cacgttatgg cctgagcgca gtagatattc tggtggaact gggcaaacgt
     5941 cggatggtgg gcggccagga agatatgatc gtcgacgtag cgctggacct gcgcaaccgc
     6001 aaataaaaaa tgattacgcg gctggcaacg tatcagccgc gattcacctg catcgtgacc
     6061 tcagagggac atcttatgac gacccgtacc tccgcatctt accgcctgac gttgaccgtt
     6121 gggctttgct ttttagttgc cctgatggaa gggctggatt tacaggccgc cggtatcgct
     6181 gccgtcggta tcgcccacgc gtttgcgctg gataaaatgc agatgggctg gatcttcagt
     6241 gcgggcattc tcgggctgct gcccggcgcg ctggtgggcg gcatgctggc cgaccgctat
     6301 ggccgcaagc gtattctcat tgcctctgtg gcgctgttcg gcctgttctc gctggcgacg
     6361 gcgatttcct gggatttctc atcgctggtg tttgcccgtc tgatgaccgg cgtcgggctg
     6421 ggcgcggcgt tgccgaatct gatcgcgctg acttccgagg cggcgggccc gcgttttcgc
     6481 ggtacggcgg tgagcctgat gtactgcggt gtgccgattg gcgcggcgct ggcggcagcg
     6541 ctcgggttta gcggcctggc ctccgcctgg caaacggtgt tctgggtagg cggggtgatc
     6601 ccgctgctgc tggtgccgct gttgatgcgc tggctgccgg aatctcaggt ttatacccaa
     6661 caaacgcagg tggccgccgc accgttgcgc gccctgtttg cgccagcgac ggcgagtgcc
     6721 acgctgctgc tgtggttgtg ctatttcttc accctgctgg tggtgtatat gctgatcaac
     6781 tggctgccga tgctgctggt ggagcagggc tttcggccat cgcaggcggc aggcgtaatg
     6841 tttgctctgc aaattggcgc ggccagcggc acgttaattc tcggggcgtt aatggataaa
     6901 ctgcgcccgg tggtgatgtc gctgctgatc tacaccggga tgttggcttc actgctggcg
     6961 ctgggtacgg tgtcgtcatt gagcggcatg ctaatggcgg gatttgtggc ggggatgttc
     7021 gccaccggcg ggcaaagtgt gttgtatgcg ctggcgccgc tgttttacag cacgcagatc
     7081 cgcgccacag gcgtgggaac cgccgtcgca gtcgggcgtc tgggggcgat gagcggtccg
     7141 ctgctggcag ggaaaatgtt agcgctcggt accggaacgg taggggtgat ggcggcttct
     7201 gcgccgggca ttttgctggc aggcctggcg gtatttattc tgatgagccg caaatcacag
     7261 atgccgatcc cggccagcgg tgcttaagca ttcatccggt tgtgggagtg gggagatttg
     7321 attcccactt ccgggttaac gccgtcagtc aactgtggtg ccaaagttta gataaaacat
     7381 ggcaacggag gcgttatgag ctggcggccc tttttgtatc tgattattaa tccccacccg
     7441 ctattaagcg cccggcgcgg gcatctgcgt ctggtgcagg gttgactttg cattctgtta
     7501 acaaacgcgg tataacaaac cttctttgga tgtttagatg tccatacgtt tagaaggtta
     7561 tatgcaaaca acacaacaaa atgcgccact gaagcgcaca atgaaaacgc gtcacctgat
     7621 tatgctttcc ttgggcggcg tgattggcac aggattattc ttcaataccg ggtacatcat
     7681 ttccaccact ggagcggcgg gaacgctgct ggcctatctg attggtgcgc tggtggtctg
     7741 gctggttatg cagtgtctgg gcgagctgtc ggtcgcgatg ccggagaccg gagcgtttca
     7801 cgtttatgcc gcgcgctatc ttggtccggc taccgggtat accgtggcct ggctttactg
     7861 gctgacctgg accgtggcgc tgggttcgag ctttaccgcc gctggattct gtatgcagta
     7921 ctggtttcca caggtgccgg tatgggtctg gtgcgtggtg ttctgcgcga ttatttttgg
     7981 tctgaatgtt atctccacgc gcttttttgc cgaaggggag ttctggttct cgctggtcaa
     8041 agtggtcact atcatcgcct ttatcatcct cggtggggcg gcgattttcg gctttattcc
     8101 gatgcaggat ggctcgcccg cgccggggct gagtaatatc acggcagaag gctggttccc
     8161 gcacggtggc ttaccgattt tgatgactat ggtggcagtg aactttgctt tttcgggtac
     8221 cgagcttatc ggcattgccg ccggtgaaac ggaaaacccg cgcaaagtta tcccggtagc
     8281 gattcgtact accatcgcgc gactgattat tttctttatc ggcaccgtgt ttgtgctggc
     8341 agcgctgatc ccgatgcagc aggcgggcgt ggagaaaagc ccgtttgtgc tggtatttga
     8401 gaaagtaggg atcccgtacg ccgctgatat ttttaacttc gtgatcctga cggctattct
     8461 ttctgcagcg aactccgggt tatatgcctc cgggcgcatg ctgtggtcgt tgtcgaatga
     8521 acgtacgcta ccggcctgtt ttgcgcgagt aacgaaaaac ggcgtgccac tgacggcgct
     8581 gtcggtcagt atgctcggtg gtgtgctggc gctgttttcc agcgtggtgg ccccggacac
     8641 ggtatttgtt gcgctgtcgg caatctccgg gtttgcggtg gtagcggtgt ggctgagtat
     8701 ctgcgcctcg cattttgttt ttcgtcgccg tcatctgcaa caaggtaagg cattgagtga
     8761 attacattat cgcgcgccgt ggtatccgct ggtgccagta ttaggttttg tgctgtgcct
     8821 ggtggcctgt gttgggctgg cattcgatcc agcgcagaga attgcgttgt ggtgcgggtt
     8881 accgtttgtt gcgttgtgct atggtgctta tttccttact caaccccgaa acgcaaaaca
     8941 ggagccagaa catgtcgcag aataatccgt tacgcgctct tcttgataaa caggatatcc
     9001 tactgctgga tggcgcgatg gcgacggagc tggaagcgcg agggtgtaac ttagccgaca
     9061 gcctgtggtc agccaaagtg ctggtagaaa acccggagct tatccgcaaa gtgcatcttg
     9121 attactaccg ggcgggggcg caatgcgcga tcactgccag ctatcaggcg acgccggcgg
     9181 gcttcgccgc gcgaggtctg gatgaagcgc agtcgaaagc gctgattggc aaaagcgtgg
     9241 agctggcgcg taaagcccgt gaagcgtatc tggcagagaa cccgcaggcg ggaacgcttc
     9301 tggtggcggg atccgtcggg ccttacggcg cgtatctggc ggatggctct gaataccgtg
     9361 gcgattatca ttgtagcgtt gaggcatttc aggcgtttca tcgcccgcgc gtggaagcct
     9421 tgctggatgc cggggccgat ctgctggcct gcgaaaccct gccgaatttt tccgagattg
     9481 aggcgttggc cgagctgttg accgcatatc cgcgtgcgcg ggcgtggttc tcatttaccc
     9541 tgcgcgacag cgaacacctg agcgacggta cgccgctgcg tgacgtggtt gcgttcctgg
     9601 cgggttatcc gcaggtggtg gcgctaggca ttaactgtat tgcgctggaa aacaccaccg
     9661 ctgcgttgca gcatttacac ggtttaacgg tgctgccgct ggtggtgtat ccgaactcgg
     9721 gcgagcatta cgatgccgtg agcaaaacct ggcatcatca cggtgaacat tgcgcgcagc
     9781 tggcggatta tctgccgcag tggcaggccg ctggcgcacg gttgattggc gggtgctgtc
     9841 gcaccacgcc tgcggatatc gccgcgttaa aagcgcgaag ctgagggttt atcgggtcta
     9901 catcgttcat tgtaggcctg ataagcgcag cgcatcaggc attgccggat ggcggcacaa
     9961 gcgccttatc cggcctacaa aaccacaaaa cgttatgccg catccgccag aacaaacatg
    10021 ccgtacggat ggatttcaag ataatactgc tcgccgacgt ccggttgcag acgcgtagcg
    10081 ttgacctgca ataatatctc ctgcccgtgc cattccaccg tcacttcata ctgcggcccc
    10141 atataggcga catggcggat cacgcagcgc tggctctctt cgccgcgatc gctgagcgtg
    10201 atcgcttccg ggcgcacacc gaccatccct tcaccctgtg taccaaagtg cagcgggcgc
    10261 ggcagatgat agccgtagat atcaacgtat ccgtcgctga aggttgccgg gaacaggttg
    10321 gcatcgccca taaagctcgc cataaagcgg gaggcgggct ggcggtaaag atcctgcggt
    10381 gagccgatct gcatgatgtg tcccttgttc atcaccagca cagtatcaga aaccgcaaag
    10441 gcttcgctct gatcgtgggt gacgtacagc gaggtgatat caaactgctt ttgcaactcg
    10501 cggatcttgt cgcgcatgct gcgacgcagg ttggcgtcga ggttactcaa cggctcatca
    10561 aacagcagca ctttcggctt gaggatcagc gcgcgggcca gcgccacgcg ctgctgctgc
    10621 ccgccggaga tctgatcgac aaagcggtct tcgaatcctt ccagatccac catcgccaac
    10681 gcctctttga cgcgggcttt cagctctgcg cgcggtacgc cgagcatttt caggccataa
    10741 ccgacattct ctcccagcga catatgcggg aacagggcat aggactgaaa caccatacag
    10801 atatcgcgct gctgaataga gcgatgggtg acgtcttcgc catcaatgaa aatttgccct
    10861 tcgctcggtt tttccagccc ggcaaccagg cgcaaaatag tggttttgcc gcagccggac
    10921 gggccgagca gcgtcaccat ttgcccctgc gggatggtga ggttgatatt gtcgattacc
    10981 gtattactgc caaatcgttt agtgacgttg cgcagttcaa cgaaattttt ctgagtcata
    11041 gtgcgctcca ttacgcctgg tttttggctt ttgaacggga ggtacgtgat tcaccgatca
    11101 gccagtcaaa gatgaaaata atcgccagca tcaccacgat cagaatggaa ccgtaggcaa
    11161 tcgctacacc gtattcgcca tcttccacgc ggttgaggat gtaagccgtc gctacgcggg
    11221 tatccggcgt gacgaggaac acaatggcgc tgacggtggt aatggcgcgc acaaagctgt
    11281 aaatcagcgc cgagaggatc gccgggcgca gcagcggcag caggatgtgc gtaatggtac
    11341 gcagggaacc ggcgcgcagg ctgagtgagg cttcatcgag cgatttatcg atttggcccg
    11401 gtaatgactc caacttattg atagtgtttt atgttcagat aatgcccgat gactttgtca
    11461 tgc
//
LOCUS       DAOFQD010000150        11454 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.150, whole genome shotgun sequence.
ACCESSION   DAOFQD010000150 DAOFQD010000000
VERSION     DAOFQD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.150"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..11007)
                     /locus_tag="QBF46_004097"
     CDS             complement(<1..11007)
                     /locus_tag="QBF46_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HDQ6676210.1"
                     /translation="MTTIKNRRLRPRAHRFALESRQLFDGAALAETAIHEASASDLVH
                     HESATEPAKALDIPHAATADAASEKPGSAASEVYVIDQHISGWQSLVDQVPQGSQVVI
                     IDENSSGLSQLADALKGETNISAIHILSHGASDAITLGTDVLTADNLSAWSSQLSAIG
                     ASLSDSGDILLYGCDVSAQDNAFITRFAELTQADVAASSDMTGSAALSGDWVLENHTG
                     SIEAKTFAFDYDGLLAGPEVTAPEKGITVAEPSSLNAAGADTATLSGWQVSTTDASET
                     VIVTVSLSNSAIGKLSDSQTSNASLTMTGTASEAQAWLNSITFTSADVELGNTGASGK
                     LDVTVRDSAGVSTSKSIDVTVTPSNDPVSVADATQNVPEIESGGSVITTVTLNAIDAE
                     VTAGTQQPVQIVYGLTDLPQYGYLTLNGDRMGVGSIFTQQDLIDGKVKYVHTATGADQ
                     NAADGFTAVINDGATPKAQSDTVHVTLNITPQNQAPTLSGSGVVFEGQPNNAVNTGNV
                     GQYIEADGGGDPGDTTLTLTITALPTHGTLWFNGVAVTVGQTFDYADRNLLTYANDGS
                     ENQGTDSFGVRVTDQGGGTGVPASTDGTITLTVRPVDDDPTLDNVTLHAQVDDLANAV
                     TLTSAMIGASDVDSTDNHLSFIVDDSKLTHGYLTLDGYRLQNGGTFTMQDIVDGKVQY
                     VQYASASASGDTDSFNFVVVDNTAALRWNADGTTYSRIGGVYTPGTETLTSFTFTIGL
                     AVVTGGGPGTIPDRVTTITNRDSTWAGVDFTSGNARGEVEEGGTLVLNGTSGDFSSVP
                     GLSYTATGVDASQVVYTFLGFSNGARGGELQKNVGGSWVTLGEYATFTQADLDSGNIR
                     FQHDGGETFVYAAAFSVSAGLTTLDANGNPVTDIWTPTLNIYVTPVNDLPVVTGSSST
                     VIAEGETAYLTTGQIAISDPDDANSSNGLEESATIKDGSASANNYAYNNDATDANALK
                     FTIKTLPDGGTLQYYNGSQWVDVTVGQTLNASLLTASASTTGLRFVSDGSEVRSTTFT
                     VSATDRWGATSASDATVKIQITNVNDAPQIAKDPTQADPTVTAGSPNLIGGTPVNNPL
                     YVTEGSYGQITSAMLQAYDSDSSSMQVQYTITGAPTHGTLAYSKDGVNFSPIGKGSSF
                     TQQDVANGYIYYLNDGTEGSGNSEPATPDDKFTFTISDGDKEQTGNAFWIYTRPTDDA
                     PEVTAPSGIKNIASATATSNALPGFSVKDGDLTDGVTDGESDYLQVTVRLLHADGSAF
                     VASEYSDVTLGYATGSGATISTAHGGQHDYLVLSGTRAQVNAALAGLTVTFRTDRNAT
                     YQVQTIADDRLRDASGTLTAGANGGTLNQPATPGRGAAATAVDATEYNWYSASVPEKS
                     GNLSAASVTIRASEINDPGTLTVTNLDRTVYEDQASFIGGNIVVQDTESAAFGTPVTV
                     TITAPSGTLGIGGADVNTSATASAAGSQPVTISGDNSGTLTLTGRASDIEALLNDAAL
                     GLTWQSGANVNHDLNGAAAGDVTITLHLDDGDSHIGDTVQADPADQTLNLTIVPVNDA
                     PVVSATGGTVYLNSDPAVVDGTKVGGFVISDPDYTDGGNIADGETDFMQVTVRITDAS
                     GVPLTAAQYSNAGVSDITISSGNTTSGVTIDSVYNGSESALVIRGTLSQVNAYLAALT
                     VSMTGSALSNADNAWRVEVIADDRLRDASGNLTSANSANGGQNDAVTGVAAVPTTAID
                     PYGALPSGLSQNVSTAWRSVFPSDVNDPATITVTDKSVNEGSTTVDLPAITVADPDAD
                     SASLTVTVTLPGGFTFKTIGGTGGTVDAGGVNTTSFTLTGTLSQINSRLANLTVNLPT
                     NAGVTAADWNGTFNVTVVVNDNANHGSRPTTLTGDSNNPNANPGDISYADDTSAALVT
                     TRTFSVTVKPSNDAPVVLGGATQTLAPVSEDTSAAAITGDTIANLFAGNFSDPNDQVN
                     NASITATGGTSADSFWGVAITNLSVNSAQGEWQYSTDGGTTWAAIGSRTDANALLLDA
                     TAKVRFVPAADFFGTPAALKVRLVETNNNGDTTATTGSLTSGQTVSTATNGSSSRYSA
                     AMVTLSTSVTNVNDRPTISATPLTMQEDTPVGKTLTSILGSYSDAADNQTGIAGGGNA
                     SGAIGYVAIVGNTTDASLGHWEYSTNGTTWTALGTSYSETNALVLLASTQLRFVPLAD
                     YNGAVPGGLTVRVSDSTSSADTGVSAAGSTQDLQSAFNSGQTSHWSTAQSISMSITAV
                     ADAVSDTAATHSGEAKTIDVLGNDSFSNSDKAITAVTQGVYGSVVIDNGKVIYTPNTT
                     SYVGSDTFTYTVTSGGVTETATVTIQMNNAAPVTQGENVSTPEDTTLTSSAAQNLLLN
                     DSDPDGDTLSILNYSVSGVGTVFTAGQTADLTGYGKLTINADGSYTFVPVADWNGTVP
                     TVTYRVVDGRTNGATTAQLKITVTPVKDAFDDSLTTHAGTPVTIDVFANDKFSNGDKA
                     ITGKTNGAYGTVSISNGQVIYTPTSGYVGTDTFTYTVTSGGKTETATVTLTLVNDAPQ
                     VGLAKTGSTAEDTTLTNGENLLTGASDLNGDALTVKSFTVGNTTWAAGQTATLAGYGA
                     LTLNADGSYTFVPVADWNGNVPQVTYTITDGRTNGDVTSRLDISVTPVQDAFNDSQTG
                     HGRDPLVIDVFNNDSFANSDKAITATTNGAHGTVTVSNGKVVYTLNDGYVGTDSFTYT
                     VTSGGKTETANVTVTLTNGAPTGVVGKTASTAEDQTLTSTALQNLLNGASDPDGDTLK
                     VSAFTVGATTWAAGQTATLAGYGALTINADGSYTFVPVADWNGSVPQVTYEVTDGRDN
                     GVQTAALDITVTPVQDAHDDSRTAHAGAPVTVDVLTNDGFTNGDKAITGVTDGAHGTV
                     SISNGQVVYTPTSGYVGNDSFTYTVTSGGIEETATVYLTLTNSAPTLPHWTVINREDS
                     PIGIPGLLLDTSSDADGDTLTVTDFTVNGTTWQSGETANLAGYGSLTITSDGSVTFIP
                     VADWNGNVPVVTYTATDGRDNGSVTSTLTIQYDAIPDAVDDNATTHAGVAKVIDVLAN
                     DRFSNSDKAVTAVTQGNNGSVAIENGKVRYTPNAGFNGTDSFTYTVTSGGVTETATVN
                     VTIANIAPVAHDDFTWTAEDTTLTRSAATGLLANDSDDDNDALAITQFSTGTQIYAAG
                     QTATLAGMGDLTINADGSYTFVPVADWNGAVQPILYQVSDGNGGFSVATLLLTVNPIA
                     DAVNDSVSTHADVPVVIDALDNDSFSNSDQTITGVTAAAHGTVTIENNTLVYRPNAGY
                     VGSDTFDYTVTSGGKTETATVTVDVTNVAPRLTSVPTISTPEDTTLNSTAAQNLLTNA
                     HDDDGDALAVTEFTVGGTRYLAGDTATLAGYGDLTIRADGSFTFVPVADWNGVPPAAT
                     YTISDGKDGGVSTATLAFSVTPVADAKDDTASTHAGVAVTIDALHNDSFSNADKAIID
                     VDQGQHGSVTIENGQLVYRPVAGYVGQDTFTYTVESGGVRETANVTVTLTNSVPVATG
                     ETVASPEDYEARGDLLLNDRDADGDPLFIAGFTVGGQTYQPGDTARLPGVGELTIERN
                     GGYRFTPVADWNGTAPVVTYTVSDGNDGGTATATLAITVTPVADVKDDSATTHAGVPV
                     TIDAIGNDRFVNPDQAITGVTQGAHGSVA"
BASE COUNT         1822 a         3312 c         3831 g         2489 t
ORIGIN      
        1 agcgacggag ccgtgcgcgc cctgggtcac gccggtaatg gcctggtccg ggttaacgaa
       61 gcggtcgttg ccgatcgcat caatcgtcac cggcacgcct gcgtgggtgg tggcgctgtc
      121 atctttcacg tccgctaccg gggtgacggt gatggcgagc gtcgcggtag cggtgccgcc
      181 gtcgttgccg tcgctgaccg tgtaggttac tactggcgcc gtgccgttcc agtcggccac
      241 cggcgtaaag cggtagccgc cgttgcgctc aatggtcagc tcgccgacgc ccgggagccg
      301 cgcggtatcg cccggctgat aagtctggcc gccgacggtg aagcccgcga taaacagcgg
      361 atcgccgtcc gcgtcacggt cgttgagcag caggtcgccg cgcgcctcgt aatcttccgg
      421 cgaagccact gtctcgcccg ttgcgaccgg cacgctgttg gtcagggtga cggtgacgtt
      481 ggcggtttcg cgaacgccgc cgctctccac ggtgtaggtg aaggtatcct ggccgacata
      541 gcctgccaca ggacgataaa ccagctggcc gttttcaatg gtcaccgagc cgtgctggcc
      601 ctgatccaca tcgataatcg ctttgtcggc attgctgaaa ctgtcgttat gcagtgcatc
      661 gatagtgacc gcaacacccg catgggtgct ggccgtatcg tccttcgcgt ccgccaccgg
      721 cgttacgcta aaggcgagcg tggcggtgga gacgccgccg tctttgccgt cgctgatggt
      781 gtaggtcgcg gcaggcggca cgccgttcca gtcggccact ggcacaaagg tgaagctgcc
      841 gtcggcgcgg atcgtcaaat cgccatagcc tgccagcgtc gcggtgtcgc ccgcgaggta
      901 gcgggtgccg ccgacggtaa actccgtcac ggcgagggcg tcgccatcgt catcgtgggc
      961 gttagtcagc aggttctgcg ccgcagtgct gttaagcgtg gtgtcttccg gcgtggaaat
     1021 cgtcggtacg ctggtcagac gcggcgccac gttggtgacg tccacggtaa cggtcgcggt
     1081 ttcggttttg ccgccgctgg tgacggtgta gtcgaaggtg tcgctgccga catagcccgc
     1141 gttcgggcgg tagacgagcg tgttgttctc aatcgtcacc gtgccgtggg cggcagcggt
     1201 gacgccggta atcgtctggt cgctgttgct gaagctgtcg ttgtcgagcg cgtcgatcac
     1261 caccggcaca tcggcatggg tgctgacgct gtcgttgacc gcatccgcga tcgggttgac
     1321 ggtaagtaag agcgtcgcca cgctaaatcc gccgttgccg tcgcttacct ggtaaaggat
     1381 cggctgcacg gcgccgttcc agtcggcgac tggcacaaag gtatagctgc cgtcggcgtt
     1441 aatggtcagg tcgcccatgc ctgcaagcgt cgcggtctgg cccgccgcgt aaatctgcgt
     1501 accggtactg aactgcgtga tggcaagagc atcattgtca tcatcgctgt cgttagccag
     1561 caacccggtg gcggcgctgc gcgtcagggt agtgtcttcc gcagtccacg taaaatcatc
     1621 gtgcgccacc ggcgcaatgt tggcgatggt gacattaacg gtcgcggttt ccgttacgcc
     1681 gccgctggtg acggtatagg taaagctgtc agtgccgtta aagcccgcgt tcggggtata
     1741 gcgcaccttg ccgttttcaa tcgccaccga gccgttattg ccctgggtga cggcggtaac
     1801 ggctttgtcg ctgttgctga atctgtcgtt cgccagcacg tcgatgactt tcgccacgcc
     1861 cgcgtgggtg gtggcgttgt cgtcaacggc gtcagggatc gcatcgtact gaatggtgag
     1921 cgtcgatgtg acgctgccgt tatcccggcc gtccgttgcc gtgtaggtca ccaccggtac
     1981 gttgccgttc cagtcagcca ccggaataaa cgtcacgctg ccgtcagaag taatagtcag
     2041 gcttccgtac cccgccagat tggccgtctc gcctgactgc caggtggtac cgttgacggt
     2101 aaaatcggtc accgtcaggg tgtcgccgtc ggcgtcgctg ctggtatcta ataaaaggcc
     2161 agggataccg atcggcgagt cttcacggtt aatcactgtc cagtggggca gcgtcggcgc
     2221 gctgttggtc aacgtcagat aaaccgtggc ggtctcctcg ataccgccgc tggtgacggt
     2281 ataggtgaag ctgtcgttgc cgacgtagcc gctggtcggg gtgtaaacca cctggccgtt
     2341 gctgatgcta accgtaccgt gcgcgccgtc ggtgacgccg gtaatcgctt tgtcgccgtt
     2401 ggtgaagccg tcgttggtca ggacatcgac cgtgaccggg gcgcccgcat gggctgtccg
     2461 gctgtcgtca tgggcgtcct gcaccggcgt gacggtaata tcgagggcgg cggtttgcac
     2521 gccgttgtcg cgcccgtcgg tgacctcata agtgacctgc ggcacgctgc cgttccagtc
     2581 ggcgaccgga acaaaggtat agctgccgtc ggcgttgatg gtcagcgcgc catagcccgc
     2641 aagcgtcgcg gtctggcctg cggcccacgt cgttgcgccc accgtaaatg cgctgacttt
     2701 cagcgtatcg ccgtccgggt cgctggcgcc gttcagcaga ttctgcaaag ccgtgctggt
     2761 cagcgtctgg tcttccgctg tgctggcggt tttgccgaca accccggtcg gcgcaccgtt
     2821 cgttaacgtt acggtgacgt tcgcggtttc ggttttgccg ccgctggtaa cggtataagt
     2881 gaaactatcg gtgccgacat agccgtcgtt cagggtgtaa accaccttgc cgttgctgac
     2941 ggtcaccgta ccgtgcgcgc cgttggtggt ggcggtaatg gctttgtcgc tgttggcgaa
     3001 gctgtcgtta ttaaagacat caatcaccag cgggtcgcgc ccgtggccgg tctggctgtc
     3061 gttaaacgca tcctgcaccg gcgtaacgga gatatcgagc ctcgaggtga cgtcgccgtt
     3121 ggtgcgaccg tcggtgatgg tgtaggtcac ctgcggcacg ttgccgttcc agtcggcgac
     3181 cggaacaaag gtatagctgc cgtcggcgtt gagggtcagc gcgccgtagc ccgcaagcgt
     3241 ggcggtctgg ccggcggccc aggtggtatt gcccaccgta aacgatttca ccgtcagcgc
     3301 gtcgccgttc aggtcgctgg cgcccgtcag cagattttcg ccgttggtaa gcgttgtgtc
     3361 ttccgccgtg ctgccggttt tagccaggcc gacctgcggc gcgtcgttga ccagagtgag
     3421 ggtgaccgtc gcggtttcgg ttttgccgcc gctggtgacg gtataggtga aggtatcggt
     3481 gccgacatag ccgctggtcg gcgtgtaaat cacctggccg ttgctgatgc taaccgtacc
     3541 gtaagcgccg ttggtcttgc cggtaatcgc tttatcgccg ttgctgaatt tatcgttggc
     3601 gaaaacgtcg atggtcaccg gcgtacccgc gtgggtcgtc aggctgtcgt caaaggcgtc
     3661 ttttaccggc gtgacggtta tcttcaactg cgccgtggtc gcgccgttgg tgcggccatc
     3721 aaccacccgg taggtcaccg tcggcaccgt accgttccag tcggcgaccg ggacaaaagt
     3781 atagctgccg tcggcgttga tggtcagttt gccatacccg gtgaggtcgg cggtctggcc
     3841 tgcggtaaac accgtgccga cgccgctgac ggagtagtta agaatgctca gcgtatcgcc
     3901 atcagggtcg gaatcgttca gcagcaggtt ttgcgcggcg gagctggtca gcgtcgtatc
     3961 ttccggcgtg ctgacgtttt caccctgggt gaccggggcg gcgttattca tctgaatcgt
     4021 gaccgtcgcg gtttcggtca cgccgccgct ggtgacggta taggtgaacg tatcgctgcc
     4081 gacgtagctc gtggtgttag gggtataaat caccttgccg ttgtcgataa ccacggaacc
     4141 ataaacgccc tgggtgaccg ccgtgatcgc tttatcgctg ttgctgaagc tgtcgttgcc
     4201 cagcacgtcg atggttttcg cctcgcccga atgggtggcg gcggtgtcgc tcaccgcgtc
     4261 cgccaccgct gtaatgctca tgctgatgct ctgcgccgtg ctccagtggc tggtctggcc
     4321 gctgttgaag gcgctttgca gatcctgcgt gctgcccgcg gcgctgacgc cggtatcggc
     4381 ggaactggtg ctgtcggaga cgcgcaccgt caggccgccc ggcaccgcgc cgttgtagtc
     4441 ggccagcggc acaaatcgca gctgggtcga ggcgagcagc accagcgcgt tggtttcgct
     4501 gtagcttgtg ccaagcgcag tccaggtcgt gccgttggta gagtactccc agtggccgag
     4561 gcttgcgtcg gtggtgttgc cgacgatcgc cacatagccg atagcgccgc tggcgttgcc
     4621 gccgccagca attcccgtct ggttgtcagc ggcgtcgctg tagctgccaa gaatagaggt
     4681 cagcgttttg ccaactggcg tatcttcctg catggtcagc ggcgtcgcgc taatcgtcgg
     4741 acggtcgttg acgttagtga cgctggttga gagggtgacc atcgctgcgc tatagcgtga
     4801 actactgccg ttggttgccg ttgatacagt ctggccgctg gtcagcgacc ctgtcgttgc
     4861 ggtggtgtcg ccgttgttgt tggtttccac cagacgtact ttcaaggcag caggcgtccc
     4921 gaagaagtca gcggcaggta caaagcggac tttggccgtt gcatccagca gcagggcgtt
     4981 ggcgtcggtg cggctgccaa tggcagccca ggtcgtgccg ccatcggtgg agtattgcca
     5041 ttcgccctgc gcgctgttga ctgacaggtt agtgatagcc acgccccaga aactgtcagc
     5101 gctggtgccg ccggttgccg taatggacgc attgttgacc tgatcgttcg ggtccgagaa
     5161 gttaccggca aacaggttgg caatggtgtc gccggtaatc gctgcggcgc tggtgtcttc
     5221 gctcaccggg gccagcgtct gggtagcgcc gcccagcacg accggggcat cgttcgacgg
     5281 tttcacggtc acgctaaagg tacgggtggt gaccagcgcg gcggaggtgt catcggcgta
     5341 gctgatgtcg cccgggttcg cgttcgggtt attgctgtcg ccggtaagcg tggtcgggcg
     5401 gctgccgtgg ttggcgttat cgttcaccac tacggtgacg ttaaaggtgc cgttccagtc
     5461 ggcggcggtg acgcccgcgt tggtcggcag gttcaccgtc aggttagcga ggcggctgtt
     5521 gatctgcgaa agcgtgccgg taagtgtgaa gctggtggtg ttgacgccgc ccgcatcaac
     5581 ggtgccgccc gtcccgccaa tcgttttaaa ggtaaagccg cccggcagag tgaccgtcac
     5641 cgtcaggctg gcgctgtcgg cgtccgggtc cgccacggta atggcgggca ggtcgacggt
     5701 cgttgagcct tcgttgactg acttgtccgt caccgtgatg gtcgccggat cgttgacgtc
     5761 gcttgggaag acgctgcgcc atgcggtact gacgttctgg ctcaggccgg aaggcagcgc
     5821 gccgtaaggg tcgatagccg tggtcggcac cgccgcgacg ccggtgacgg catcgttctg
     5881 gccgccgttg gcgctgttgg cgcttgtcag gttgccagag gcgtcgcgca ggcgatcgtc
     5941 ggcaatcact tccacacgcc aggcgttatc ggcgttcgac agggcggagc cggtcatgga
     6001 gacggtcagc gccgccaggt acgcgttcac ctggctcagc gtgccgcgga tcaccagtgc
     6061 ggactcgctg ccgttataga cgctgtcaat cgtcacgccg gacgttgtgt tgccggagct
     6121 aatggtgatg tcggatacgc cggcgttact gtactgcgcg gcggtcagcg gcacgccgct
     6181 ggcgtcggtg atgcgcaccg tcacctgcat aaaatcggtt tcgccgtcgg cgatattgcc
     6241 gccgtcggtg taatccgggt cggaaattac aaagccgccg actttggtgc cgtcgactac
     6301 cgccggatcg ctgttgaggt aaaccgtacc gcccgttgca gagacaaccg gcgcgtcgtt
     6361 caccggcaca atggtgaggt tcagcgtctg gtcggccggg tcggcctgca cggtgtcgcc
     6421 gatatgcgag tcgccgtcgt ccagatgcag ggtgatggtg acatcgcccg ccgctgcgcc
     6481 gttcaggtcg tggttgacgt tggcgccgct ctgccaggtg aggccgagcg ccgcgtcgtt
     6541 cagcagcgct tcaatgtcgc tggcgcgacc ggtcagcgtc agcgtgccgg agttgtcgcc
     6601 ggaaatcgta accggctggc tgccggcggc gctggcggtg gcgctggtgt tcacgtccgc
     6661 gccgccgatg ccgagggtgc cggacggcgc ggtgatggtg acggtcaccg gcgtgccgaa
     6721 ggcggcggat tcggtgtcct gcaccacgat attgccgccg ataaagcttg cctgatcttc
     6781 ataaacggta cggtccaggt tggtcacggt cagcgtgccc ggatcgttga tttccgaggc
     6841 gcgaatggtg acggacgccg cgctcaggtt gccgcttttt tccggtacgg aggcgctgta
     6901 ccagttgtac tcggtggcgt cgacggcggt ggccgccgcg cctctgccag gcgtcgcggg
     6961 ctggttcagc gtgccgccgt tagcgcctgc cgtcagcgtg ccgcttgcgt cgcgcaggcg
     7021 gtcatcggcg atagtctgca cctgataggt ggcgttgcgg tcggttctga aggtcaccgt
     7081 caggcccgcc agcgccgcgt tcacctgggc gcgggtgccg ctcaagacca gatagtcgtg
     7141 ctggccgccg tgtgcggtag agatcgtcgc gccgctgccg gtcgcgtaac ccagggtcac
     7201 gtcgctgtat tcgctggcca cgaaggcgct gccgtccgca tgcagcagac gcacggtcac
     7261 ctgcaaataa tcgctctcgc catcggtcac gccgtctgtc agatccccat ctttgaccga
     7321 gaagcccggc agggcgttgc tcgtcgccgt ggcgctggcg atgttcttga tgcccgacgg
     7381 cgcggtcact tccggcgcgt cgtcggtcgg gcgggtgtag atccagaacg cgttgccggt
     7441 ctgctctttg tcgccgtcag aaatcgtgaa ggtgaactta tcgtccgggg tggcgggttc
     7501 gctgttgccg ctgccttcgg tgccgtcgtt caggtagtaa atgtacccgt tcgccacatc
     7561 ctgctgggtg aaggaggagc ctttgccgat ggggctgaag ttcacaccat ctttggaata
     7621 ggccagggtg ccgtgggtcg gcgcgccggt gatggtgtac tgcacctgca ttgagctgct
     7681 gtcggaatcg taggcttgca gcatggcgct ggttatctgg ccatagcttc cttcggtgac
     7741 gtacagcggg ttgttgactg gcgtgccgcc aatcaggttc ggcgagcctg cggtcaccgt
     7801 cgggtcggcc tgggtcgggt ctttggcgat ctgcggcgcg tcgttgacgt tagttatctg
     7861 gattttcacc gtcgcgtcgc tggcggaggt ggcgccccag cggtcggtgg ccgatacggt
     7921 aaaggtggtg gagcgcactt ccgagccgtc gctgacgaag cgcagcccgg tggtcgaggc
     7981 gctggcggta agcagcgagg cgttgagcgt ctggccgacg gtaacgtcaa cccactgcga
     8041 gccgttgtag tattgcagcg tgccgccgtc cggcagcgtt ttgatggtga acttcagggc
     8101 gttggcgtcg gtcgcgtcgt tgttataggc atagttgttg gcgctggcgc tgccgtcttt
     8161 gatcgtcgcg ctctcttcca gcccgttgct gctgttggcg tcgtccgggt cgcttatcgc
     8221 tatctggccg gtggtcaggt aagcggtttc gccttcggcg atgacggtgg aggaggagcc
     8281 ggtcaccacc ggcagatcgt tcactggcgt gacgtagatg ttcagcgtcg gcgtccagat
     8341 atcggtcacc gggttgccgt tggcatcgag cgtggtcaga cccgccgaga cagagaacgc
     8401 cgcggcgtag acaaaggtct ctccgccgtc gtgctggaag cgaatattgc cgctgtcgag
     8461 gtcggcctgc gtaaaggtgg catattcgcc gagcgtcacc cagctgccgc cgacattttt
     8521 ttgcagctcg ccgcctcttg cgccgttcga gaaaccgagg aaggtataga ccacctgcga
     8581 ggcgtccacg cccgtggcag tgtagctcag gcccggcacg ctggagaagt cgccgctggt
     8641 gccgttcagc acgagggtgc cgccttcttc tacttcgccg cgggcgttgc cggaggtgaa
     8701 gtccacgccc gcccaggtgg agtcgcggtt ggtgatggtg gtgacgcgat ccgggatcgt
     8761 gccgggaccg ccgcccgtga ccaccgcaag gccgatggta aaggtaaagc tggtcagcgt
     8821 ttcggtgccc ggcgtgtaca ccccgccgat gcggctgtag gtcgtgccgt cggcgttcca
     8881 gcgcagcgca gcggtgttgt ccaccaccac aaagttgaag ctgtcggtgt cacccgacgc
     8941 gctggcgctg gcgtactgca cgtactgcac tttgccgtcg acgatatcct gcatggtgaa
     9001 ggtgccgccg ttttgcaggc ggtagccgtc cagcgtcagg taaccgtggg tgagcttgct
     9061 gtcgtcaacg ataaagctga ggtggttatc ggtggaatcc acgtcgcttg cgccgatcat
     9121 cgcagacgta agggtgacgg cgttcgccag atcgtccacc tgcgcgtgca gcgttacgtt
     9181 gtccagagtc gggtcgtcat ccaccgggcg cacggtcagg gtaatggtgc cgtcggtgct
     9241 ggccggcacg ccggtaccgc cgccctggtc ggtgacgcgt acgccaaagc tgtcggtgcc
     9301 ctggttttca ctgccgtcgt tagcataggt cagcagattg cgatcggcat agtcaaaggt
     9361 ctggccgacg gtgacggcga cgccgttaaa ccagagcgtg ccgtgggtcg gcagggcggt
     9421 aatggtgaga gtcagcgttg tgtcgcccgg atcgccgccg ccgtccgcct ctatatactg
     9481 gccgacgttg ccggtgttga ccgcattgtt gggctggcct tcaaagacca cgccgctgcc
     9541 gctaagggtc ggcgcctggt tctgcggggt aatgttcagc gtgacgtgga cggtatcgga
     9601 ctgcgccttc ggcgtcgcgc cgtcgttgat gaccgccgta aagccgtcgg cggcgttctg
     9661 gtctgcgccc gtggcggtgt ggacatactt caccttgccg tcgatcaaat cctgctgggt
     9721 aaagatagag ccgacgccca tccggtcgcc gttcagcgtc agatagccat actgcggcag
     9781 gtcggtcaga ccatagacaa tctgcacggg ctgctgggtg cccgccgtta cttccgcatc
     9841 gatggcgtta agggtcacgg tggtgatgac gctgccgccc gattcgatct ccggcacgtt
     9901 ctgggtggcg tcggccacgc ttaccgggtc gttcgacggg gtcacggtca catcgatgga
     9961 tttgctggtg ctgacgcccg cgctgtcgcg caccgtgacg tccagcttgc cgctggcgcc
    10021 ggtgttgcca agctcgacgt cagcggaggt aaaggtgatg ctgttaagcc aggcctgcgc
    10081 ttcgctggca gtgccggtca tggtcagcga agcgttgctg gtctggctgt cgctgagttt
    10141 gccgatggcg ctgttgctca ggctcaccgt gactatgacg gtttcgctgg cgtccgtggt
    10201 gctgacctgc cagccgctga gcgtggcggt atcggcgcct gcggcgttaa ggctgctcgg
    10261 ctcggcgacg gtgatgcctt tttccggcgc ggtgacttcc ggcccggcca gcaggccgtc
    10321 gtaatcaaag gcgaaggttt tcgcttcaat gctgccggta tggttttcca gcacccagtc
    10381 gccggaaagc gccgcgctgc cggtcatgtc gctggaggcc gccacgtccg cctgagtcag
    10441 ttcggcgaag cgggtgataa aggcgttatc ctgggcggag acgtcgcagc cgtaaagcag
    10501 gatgtcgccg ctgtcgctaa ggctcgcgcc gatagcgctg agctgtgaag accacgccga
    10561 gaggttgtcc gccgtcagca catcggtgcc aagcgtaatg gcgtcggacg cgccgtggct
    10621 cagaatatga atggcggaaa tattggtttc gcctttcagc gcgtcggcaa gctggctcag
    10681 acccgagctg ttttcatcga taatcaccac ctggctgccc tgcggcacct ggtccaccag
    10741 cgactgccag ccggagatat gctggtctat tacatagact tcgctggcgg cgctgcccgg
    10801 tttttcgctt gcggcgtcgg ctgtggcggc gtggggaata tccagcgcct tcgcgggttc
    10861 ggtggcgctt tcatggtgca ccagatcgct tgccgatgcc tcatggatgg cggtttcagc
    10921 cagagcggct ccatcaaaaa gctggcgaga ctcaagagca aaacgatgcg cacgcggacg
    10981 taaacgacgg ttctttattg tagtcatagg tttactgctc ttaaaaaata gagtgactgg
    11041 ataaatattc tgagttcagc gttgccagga gcgtacacca tgtcgccagg ccagaggagg
    11101 gcaaacaggg gatatattca tcgaaattat cgaatggacg cttagcgctc gaagaataaa
    11161 taaatcttaa aggacaaaca agacagcgcc ctgccagccc tgaaaaaacc aatacgacga
    11221 aaggcttaac cattagctta gcatttgaat actttgaaaa caattcgctt ttagtaacaa
    11281 ttaagcaacg ctgaaaaccg aaaaggtaaa gcaaaagttc ggcgcattag aattaaatac
    11341 taacttcatg aaaaattcag gttattattt tattaattcc cgttaccgca ttagtgaatt
    11401 atgatttttt ataaattgcg caaaaaaaag agcaaaatct gcttggcctc tcaa
//
LOCUS       DAOFQD010000151        11272 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.151, whole genome shotgun sequence.
ACCESSION   DAOFQD010000151 DAOFQD010000000
VERSION     DAOFQD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.151"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            33..425
                     /locus_tag="QBF46_004098"
     CDS             33..425
                     /locus_tag="QBF46_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676211.1"
                     /translation="MSDKVTVKQTINKATSIYKIEQITVGKPGSEQYRRAFELADQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHE
                     DGGIGYQFLYEGDELKCYEHGLLIESVE"
     gene            761..1645
                     /locus_tag="QBF46_004099"
     CDS             761..1645
                     /locus_tag="QBF46_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDQ6676212.1"
                     /translation="MPEESKGFLSVEEVAGNTGEIHSLKPNNNSTIQPIALLRLGVFV
                     PTLKSTNVALRRGSSVTTNTTNATEELSSLKIVEQEGYEGIEIHGPRLDMDTDFKVWV
                     GITSALFDYAPDDDGIITLPFSEFADRCGYPRKRLSKAFRKSIDDSLTRIQQTVVKFR
                     FPAAKGHLNNINVNLLAYSSLNTELDVIEIQPQKQLSELYYVDYKRILKLKMLDKLGR
                     KETAKVLYTFFEALPANPAPVSIERLRARLNLKSSVSVQNSVIRKAMKDLEAIEYLKF
                     SEIKNGRKIGFQIHKRNP"
     gene            1938..2747
                     /locus_tag="QBF46_004100"
     CDS             1938..2747
                     /locus_tag="QBF46_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDQ6676213.1"
                     /translation="MQEEKQHYLYVLVPENGDTFKIGISCGPLARFKGLQVSPDFALS
                     RVYRGTRLAMVNLERALHATFFPWNAPWGKSAGGGHTEWFTRECLDKVLAHIEYLNDM
                     WGGILERIKSNDLLQRPVDAACSFEKELDVTSIVTFKDDAGMRDVAYVSISGYEPDAI
                     RAQCELLKAMFMLRTKYPWDTGRVCFPMEELTATIDSQLYHDNPEKFFSLLAGNGLNC
                     VSGLGRSRIQHASLFGPFFYDRHGYFEAELPALTRAIDTIDFERLFAALSK"
     gene            2916..4112
                     /locus_tag="QBF46_004101"
     CDS             2916..4112
                     /locus_tag="QBF46_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6676214.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            4129..5130
                     /locus_tag="QBF46_004102"
     CDS             4129..5130
                     /locus_tag="QBF46_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDQ6676215.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     gene            5353..7059
                     /locus_tag="QBF46_004103"
     CDS             5353..7059
                     /locus_tag="QBF46_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676216.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDSFDIALPVNQSFDV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            7120..8709
                     /locus_tag="QBF46_004104"
     CDS             7120..8709
                     /locus_tag="QBF46_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676217.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYEDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPVIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSKISTEAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGNICSDRLIDGFFDVKPTLTYTEALSAVEDTGLLGTDYVSCAVYHFPFSCKD
                     KWTQSRVVFGLSGAAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            8719..9534
                     /locus_tag="QBF46_004105"
     CDS             8719..9534
                     /locus_tag="QBF46_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676218.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEPPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKERRKAQTGAAKLARKKSMKLRRKRLG"
     gene            9570..10151
                     /locus_tag="QBF46_004106"
     CDS             9570..10151
                     /locus_tag="QBF46_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676219.1"
                     /translation="MAPIPYGVYSQADGVSPYLKVTLTNSQYQVTGYISQGAAMNMAQ
                     NWEAPFTGMSMGSVAGAFSGFAQVGTETTSVARWNSLMVWEGGTPPTFTLPVTFIALF
                     DPFTEVSGAIAALSAMISPELKDASIGGRIPERVTLNIGRRINIIDVAIQDISFDLDA
                     PRDSNGHFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            10163..10672
                     /locus_tag="QBF46_004107"
     CDS             10163..10672
                     /locus_tag="QBF46_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDQ6676220.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTFAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIDWD"
     gene            10832..>11272
                     /locus_tag="QBF46_004108"
     CDS             10832..>11272
                     /locus_tag="QBF46_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDQ6676221.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFEL"
BASE COUNT         2943 a         2612 c         2786 g         2931 t
ORIGIN      
        1 cgagctatta gcggtaacta aaagggattt ttatgtctga taaagtaaca gtaaagcaaa
       61 ctatcaacaa agcgacttca atctacaaaa ttgagcaaat cactgttggc aagccaggat
      121 ctgagcaata ccgtcgtgct ttcgagcttg ccgatcagct tggtttaaaa cacccggatt
      181 gcattgagca tgtatttccg acctatgctg atgagcaatg tactcatgtt cttaccgaag
      241 aggatttttt cagcactgaa gaacgagaag gcgttgatcg ctgcattggt gtgatttgct
      301 cttcagtgag ttatgagtta ttccctaatg tccatgaaga tggtggtatt ggataccaat
      361 tcctgtacga aggcgatgag cttaaatgtt atgaacatgg tcttcttatc gaaagcgtag
      421 aataatatga cttccttcca accggctttg ttggccggtt tattcaactt atccacagca
      481 tagatccaat aaacagatcc taaagagaac ctaggaagat ccaaagaaga tcccggatcg
      541 ctgtaagccg cgtcattact ggcctgaaat gggatcaaca ttgactatac gcgattttat
      601 gttgactgtg cacgatttat tgttgactgc acgcgattta ttgttgacta tacgcgacag
      661 aaacattgac tgtaagcgat tttagagtct gaatattcac agctgttgat aacagccatc
      721 caaattgacg tcaggccgcg ccacacatgg agaaaccgcg atgccggaag aaagtaaagg
      781 cttccttagc gttgaagaag ttgcaggaaa tacaggagaa atccacagcc tgaagcccaa
      841 taacaacagc actatacaac ccatcgcttt gttgcgctta ggtgtgtttg tgccaacctt
      901 aaaatctacc aatgtggcgc tacgtcgcgg atcgtcagtt actacaaaca caacgaacgc
      961 aaccgaagaa ctatcaagcc tcaaaattgt tgagcaggaa ggctatgagg gaattgaaat
     1021 tcatggtcca cgcctggata tggatactga ttttaaggtg tgggtgggca taacctccgc
     1081 gttgtttgac tacgcacctg atgatgacgg cataatcacc ctgccattct ccgagtttgc
     1141 cgatcgatgc ggctatccac gtaagcgcct ttcaaaggcg ttccgtaaaa gtattgatga
     1201 ctctctgaca cgcattcagc agacagttgt caaattccgc ttcccggcgg caaaaggtca
     1261 tctcaataac attaacgtca acttgttggc atatagcagc ctgaataccg agcttgatgt
     1321 tatcgagatc cagccgcaga aacagctatc tgaactttac tatgttgact ataagcgaat
     1381 cctgaagctg aagatgctgg ataaactagg gcgcaaagag acggccaagg tactgtatac
     1441 attctttgag gctctacccg ccaacccggc acctgtcagc attgagcgcc ttagagcgag
     1501 gcttaatctc aaatcatccg ttagcgtgca aaatagcgtt atcagaaaag ccatgaaaga
     1561 tttggaagct attgaatatc ttaaattttc agagataaaa aacggcagga aaatcggctt
     1621 ccagatccat aagcgcaatc cataatattg actatatgcg atagcgagaa gttgactata
     1681 ggcgacattc gttgacgctg gtggattttt gctggcgtca atattctgca agtcgctatt
     1741 gagatggctt ttaggggcat ttcatcgcgt atagtcaacg tttctcccga caatatctta
     1801 catagtcgat ctttggtgga gttaaatcga ctacagtcaa cttttgactg tagtcacatc
     1861 gcgcatagtc aactattcac attaaccttc gcgcatagtc aacatttgcg cggttctcat
     1921 caagcagtgg tattgatatg caagaagaga aacaacacta cctctacgtt ctggtgccag
     1981 agaacggaga tacttttaaa atcggcattt catgtggtcc attggcacgg tttaaagggc
     2041 tacaagtgag tcccgatttt gcgctttcac gggtctatcg tggtacgcgt ttggcaatgg
     2101 ttaatcttga gcgggcttta cacgcaacct ttttcccctg gaatgcgccg tgggggaaaa
     2161 gcgccggtgg cgggcatact gaatggttta cacgagagtg tcttgataag gttttggctc
     2221 atatcgaata tctaaatgat atgtggggag ggattctcga gcgcattaag tcgaatgatt
     2281 tacttcagcg tccagtagat gctgcttgct ctttcgaaaa agagctggat gttacttcta
     2341 tcgtgacttt caaagatgac gcaggaatga gggacgtggc ttatgtctcc atatctggct
     2401 atgaaccgga cgcgatccgc gctcaatgtg aattgctgaa agcaatgttt atgcttcgga
     2461 ctaaatatcc ctgggataca gggcgggtgt gcttccctat ggaagagtta accgccacca
     2521 ttgattccca gctttaccac gataatccag agaagttttt tagcctttta gccggtaatg
     2581 ggcttaactg tgtgtccggg ctggggcgaa gtagaatcca acatgcctcg ctcttcggtc
     2641 ccttctttta cgatcgacac gggtactttg aggctgaact tccggcgctt acgcgtgcta
     2701 tagatactat cgatttcgaa cgattattcg ctgctcttag caaataacac tgatgcccct
     2761 gaacggggct tttttgtgcc ctccttgtaa ctctcaatcg tgcaaaatga accaaacatg
     2821 cagagaatgc tatgtacaag catctacgca tacattatta ttttatgcag catttttaat
     2881 taaattcaaa aatacagcat aaaggatgac tttcgatgag tgattccagc cagcttcaca
     2941 aggttgctca aagagcaaac agaatgctca atgttctgac tgaacaagta cagttgcaaa
     3001 aggatgagct acacgcgaac gagttttacc aggtctatgc gaaagcggca ctggcaaaat
     3061 tgcctctact gactcgagcg aacgttgact atgccgtgag tgaaatggaa gaaaagggtt
     3121 atgttttcga taaacgccct gctggctctt caatgaaata tgcgatgtca attcagaaca
     3181 tcattgacat atatgaacat cgcggagtgc caaaataccg ggatcgctac agcgaagcgt
     3241 atgtgatttt catctccaat cttaaaggcg gtgtgtcaaa aactgtatcg acggtttctc
     3301 tggcgcatgc aatgcgtgct caccctcatc ttctgatgga agatttaagg attctggtta
     3361 ttgaccttga tccgcaatct tcagcaacga tgtttttaag ccataaacac tctattggta
     3421 tcgtaaacgc aacatctgca caggctatgt tgcagaatgt aagccgtgaa gagctgttag
     3481 aggagtttat tgttccttct gttgtacctg gggttgacgt tatgcctgcg tcgattgacg
     3541 atgcctttat tgcatccgat tggagagagc tgtgcaatga gcatctaccg ggtcagaaca
     3601 tccatgctgt cctgaaagaa aatgtgattg ataagctgaa gagcgattat gactttatcc
     3661 tcgttgatag tggtcctcac cttgacgcct tcctgaaaaa tgctttggcc tcggccaata
     3721 tactgtttac acctttgccg cctgcaacgg ttgatttcca ctcatcgctt aaatacgttg
     3781 cccgccttcc tgagttggtg aaactcattt cggatgaagg ctgcgagtgc cagcttgcga
     3841 ctaacattgg ttttatgtcc aagttgagta acaaggcaga ccataagtat tgccatagcc
     3901 tggctaaaga agtgttcggt ggggatatgc ttgatgtcgt cctccctcgc cttgacggtt
     3961 ttgaacgctg cggcgagtct tttgacactg ttatttcagc taacccggca acgtatgttg
     4021 gtagtgctga tgcattgaag aacgcgcgaa ttgccgcgga agattttgct aaagcagttt
     4081 tcgaccgtat tgaatttatc agatctaact gaggagtaag aaacccccat gtcaaagaaa
     4141 aacagaccaa caattgggcg aacccttaat ccttcaatat taagcggatt tgatagttct
     4201 tcagcctctg gcgatcgagt cgagcaggta ttcaagttat caactggtcg ccaggccaca
     4261 tttattgaag aggtaatacc tccgaaccag gtagaaagcg atacctttgt tgatcagcat
     4321 aacaacgggc gtgaccaggc atctcttacg ccaaaatcat taaaaagtat ccgaagcact
     4381 attaagcatc agcaatttta ccctgcaata ggtgttagac gggctacagg gaaaattgaa
     4441 attttggatg gttcccggcg tcgagcttct gccatcttag agaacgtagg gttgcgggtt
     4501 ttagtcacgg accaggagat cagcgttcag gaagcgcaaa atttagcgaa agacgttcag
     4561 acagcattgc agcacagcat tcgagaaata ggtctgcgtt tgatgcgaat gaaaaatgat
     4621 gggatgagtc agaaggatat tgcagccaaa gaagggctgt ctcaggcgaa ggtcacgcgt
     4681 gctctccagg cagcgagtgc tccggaagaa ttagtcgccc ttttccctgt gcagtcggaa
     4741 ttaacctttt cggactacaa aacgctttgt gctgttggcg acgaaatggg gaacaagaat
     4801 ttagagtttg atcagcttat tcaaaacata tccccggaaa taaacgacat cttatccatt
     4861 gaagaaatgg ccgaagatga agttaaaaat aaaatcctgc gcttgataac aaaggaagcc
     4921 tcactactca cggataaagg ttctaaagat aagtccgtag ttactgaatt atggaaattt
     4981 gaggacaagg atcgctttgc aaggaagcgc gtgaaaggcc gtgcattttc ttatgagttt
     5041 aatcgactct caaaagagtt acaggaagaa ctcgacagga tgattgggca tatccttaga
     5101 aagagcctcg ataaaaagcc gaagccttaa actttcgcca ttcaaatttc accattaaat
     5161 tactgttttt aaagtaaatc cttctaaaat ttcaaggtga aatcgccacg atttcacctt
     5221 ggattttacc ttttcccccc tcctcccgaa aaaaaaaaat ttcttgtcac gagaaagtca
     5281 acaagtgact ttcaataaaa tctcttccga aaagggattc acacaagtgc cttgtgttta
     5341 aggaagagta aattgagtaa cttacgcgaa taccagaatc gtattgcaga tatcgcaaaa
     5401 cgctctaaag ctgtgcttgg ctgggcaagc actgcgcagt tcggtactga taaccaattc
     5461 attaaagatg atgccgcgcg tgccgcatct atccttgaag ctgcacgtaa agacccggtt
     5521 tttgcgggta tctctgataa tgccaccgct caaatcgcta cagcgtgggc aagtgcactg
     5581 gctgactacg ccgcagcaca taaatctatg ccgcgtccgg aaattctggc ctcctgccac
     5641 cagacgctgg aaaactgcct gattgagtcc acccgcaata gcatggatgc cactaataaa
     5701 gcgatgctgg aatccgtcgc agcagagatg atgagcgttt ctgacggtgt tatgcgtctg
     5761 cctttattcc tcgcgatgat cctgcctgtt cagttggggg cagctaccgc tgatgcgtgt
     5821 accttcattc cggttacgcg tgaccagtcc gacatctatg aagtctttaa cgtggcaggt
     5881 tcctcttttg gttcttatgc tgctggtgat gttctggaca tgcaatccgt cggtgtgtac
     5941 agccagttac gtcgccgcta tgtgctggtg gcaagctccg atggcaccag caaaaccgca
     6001 accttcaaga tggaagactt cgaaggccag aatgtaccaa tccgaaaagg tcgcactaac
     6061 atctacgtta accgtattaa gtctgttgtt gataacggtt ccggcagcct acttcactcg
     6121 tttactaatg ctgctggtga gcaaatcact gttacctgct ctctgaacta caacattggt
     6181 cagattgccc tgtcgttctc caaagcgccg gataaaggca ctgagatcgc aattgagacg
     6241 gaaatcaata ttgaagccgc tcctgagctg atcccgctga tcaaccacga aatgaagaaa
     6301 tacaccctgt tcccaagcca gttcgttatc gcggctgagc atacggtaca ggcggcgtat
     6361 gaagcacagc gtgaatttgg tctggacctg ggttccctac agttccgcac cctgaaggaa
     6421 tacctgtctc atgaacagga tatgctgcgt cttcgcatta tgatctggcg tactcttgcg
     6481 accgactcct ttgacatcgc tttgccggtt aaccagtcct ttgatgtatg ggcaaccatc
     6541 attcgtggca aattccagac tgtatatcgc gacattattg agcgcgttaa atcttctggt
     6601 gcgatgggga tgtttgctgg tgctgatgca gcatctttct tcaaacagtt gccgaaggat
     6661 ttcttccagc cagccgaaga ctatatccag actccgtatg ttcactacat cggtaccctg
     6721 ttcggtaacg tgaaagtgta cgaagtacct gctggtattt gtaagaattt aacgacagag
     6781 aacattcagt tcagctcgat ggatgtgctg tgctacgtcc gtgatgaaaa tccgggtaaa
     6841 gcaggcttcg tgactggtga tgctgtcccg gctatcccgt tccagcatcc gaccactccg
     6901 gcgctggtca accgtaccac gctgtggggt tcggctatca acgatatgca cccacgcaac
     6961 ggcgctgatt acttcactcg tgtaacgctg acaatggcca aaaaaggcgg gcttaacttt
     7021 ataagcggcg acacgattga tgccggtgac tctgagtaat caggggaagt tctccgttta
     7081 acatagcgcc cccgtgcggg gcgcataaca gggaaagtta tgtctcaata ttcaattcaa
     7141 cagtcattag gtaatgcatc cggcgtcgcg gttagcccga tcaatgccga tgcgacgtta
     7201 tctacaggtg ttgcattaaa tagcagcttg tgggctggta ttggcgtatt tgcgcgtggc
     7261 aagccgttta ctgttcttgc ggttactgag tccaattacg aagatgttct cggcgaaccg
     7321 ctgaagccgt cttccggctc acagttcgaa ccaattcgcc atgtatacga agctattcag
     7381 caaacgtctg gttatgttgt ccgtgctgtt ccggatgatg cgaagttccc ggttattatg
     7441 ttcgatgaat caggcgaacc ggcttacagt gcgttgccat acggttctga aattgaactt
     7501 gatagcggcg aagcctttgc tatctacgtt gatgatggtg atccgtgtat ttcacctacc
     7561 cgtgagttaa ccattgaaac ggcaacagcg gacagcgcag gtaatgaacg cttcctcctg
     7621 aaactgaccc agacgacttc gctcggcgtg gtaacgaccc tggagacaca cactgtgtct
     7681 ttggcggaag aagcgaaaga tgacatgggc cgcttgtgtt atctgcctac ggctctggaa
     7741 gcccgttcta aatatctgcg cgcggttgtt aatgaagagc tgatttcgac cgcgaaagta
     7801 acaaataaaa aatcactggc gttcactggc ggtaccaacg gcgatcagtc gaaaatctcc
     7861 accgaagcct acctgcgtgc ggttaaggtg ctgaacaatg cgccgtacat gtacaccgct
     7921 gttctcggcc tgggttgcta tgacaatgcg gcgatcaccg cgttaggtaa tatctgttct
     7981 gatcgcctga ttgatggctt ctttgatgtc aaaccgacat tgacgtatac ggaagcgctc
     8041 tctgctgttg aagataccgg tttacttggt accgattatg taagctgtgc tgtctatcac
     8101 ttcccgttct cctgcaaaga caaatggacc caatcccgtg tggtcttcgg tctgtctggc
     8161 gcggcgtatg cggcgaaagc tcgtggcgtc aagaaaaact ctgatgtcgg cggttggcat
     8221 tactcaccgg ctggtgaaga acgtgccgtc attgctcgtg cgtcaattca accgctgtat
     8281 ccggaagata ccccggacga agaagcaatg gttaagggcc gcctcaataa agtatctgtt
     8341 ggcacctctg gccagatgat catcgacgat gctttaactt gctgcacgca ggataactat
     8401 ctgcacttcc agcacgtccc atccctgatg aatgcaatca gccgtttctt tgtccagtta
     8461 gcccgccaga tgaagcatag ccctgacggt attactgccg ctggtctgac taaagggatg
     8521 accaaacttt tggatcgctt tgtcgcctcc ggcgctctgg tggctcctcg tgatcctgat
     8581 gctgacggta cagaaccgta tgtgctgaaa gttacgcagg cggaattcga taaatgggaa
     8641 gtagtctggg cctgctgccc gactggcgta gcccgtcgta tccagggcgt accgctgctt
     8701 attaagtaag ggaatacaat gagcaaaaac ttttttcaat ccggggcatt tttggggaat
     8761 ggactgtccc gtttcgcttt gaactctgat cctgtgcagc tgatggagtc tgcccgagca
     8821 agcgccgaac cgccaacaga tccggttatt aataataatc cggaaccggc ggcacagact
     8881 aacgataacg ttccatctgc cccggctcct gagcaaatcc tggaagggaa agacggtaaa
     8941 gaatggaccg tcgaacaggc gcaccagatg attctggaag ctgcaaatcg aagtgctatg
     9001 cagaatgcgt tgagtgatgc ggccgacgcc gttttcgcct gggctgatag cggtgatctg
     9061 actttcgact cccttgatgg tttcgttcag gctatcgctg gtatctctga tgacgacgac
     9121 tccgaagtta cagaagaaca ggacgatgcc tataacgaag catgggcaaa tgttgctgac
     9181 ttcctcgcag catgcggtgt agatgatgac ctgatcgaag cactggctga cgatgaagac
     9241 gacgacgcag ccgctgatgt tggtgcctct atcgctggtt tagatagcga cgaccgtgac
     9301 gaactggaag cggcgtttgt tgttgctggc acttctgatg aaatgctgac tgaagcattt
     9361 aagaaggttg ttcgtaacgg tgagatcaaa ctcatccgta aacgcctgcg taaaaaacgt
     9421 ctgactgcgg ctcaaaaatc ggcgctgaaa aaagagcgtc gtaaagccca gaccggcgcg
     9481 gcaaaactgg cccgcaaaaa gtcaatgaaa ctgcgccgta agcgcctcgg ctaaaggagg
     9541 aggccggaga actccggcct ttaacttgaa tggcacctat accttatggg gtttacagcc
     9601 aggctgacgg tgtatcgcca tatctgaaag ttactttaac gaactctcag taccaggtta
     9661 ccggatatat cagccaggga gcggcaatga acatggccca gaattgggaa gcgccgttta
     9721 ccggtatgtc catgggatct gttgctggtg ccttcagtgg ttttgcgcag gttggtactg
     9781 aaacaacgtc ggttgcccgt tggaacagct taatggtttg ggaaggggga acaccgccga
     9841 ctttcacgct gccagtaact ttcatcgctt tgtttgaccc attcacggag gtttcaggag
     9901 ctatcgccgc attgtcagcg atgattagcc cggaacttaa agatgccagt attggtggtc
     9961 gaatcccgga gcgtgtgacg ctaaacattg gtcgccggat caacatcatt gatgtcgcta
    10021 tccaggacat aagtttcgat ctcgatgcgc ccagggacag caatgggcat ttcctgaaaa
    10081 acaccgtcaa cctccagttg accggttctt cgatatataa cagctccgat attgttcggg
    10141 cgttccagta aaaggatttt atatggggca caataacact aagggaaacc gtaaatttat
    10201 taagggccgc tatactgcca acgcggccaa aggcgaacga ctggtatctt ctgaattcca
    10261 gctcactttt gcaggccatg aagatatcag cgtactggtt cgcacgtcgc aaattcctga
    10321 aatgacccgc gaggatgtgg aggactatgg tccgaatggt gtgaagttca accagcacgg
    10381 tccaatccgt aactccgggg aaatccaggt ccagtgcgtg gagactatcg aaggcgatat
    10441 tcttcagttc attaaagatc gcattgcggc gaaggactat gttgatatca cgatggctgc
    10501 tacccctgaa tccaaatctt ccggggttaa cgctgtgaca aaagctgcta caacaattga
    10561 aatgttggac tgcaaaatct acagtgatgc aatcgacttt agtaccgaag atgtgactgc
    10621 cgctgtgcgc ccgtcacttc gtatcgtcta caactggatt gattgggatt aagagtcatc
    10681 ccttgtattt taaagctcct tcgggagctt ttttatttgg agaggaaagg gtgcattgag
    10741 gatacctgac acacgaagag tggcggggat ctctcccata agcgctaact taagggttgt
    10801 ggtattacgc ctgatatgat ttaacgtgcc gatgaattac tctcacgata actggtcagc
    10861 aattctggcc catattggta agcccgaaga actggatact tcggcacgta atgccggggc
    10921 tctaacccgc cgccgcgaaa ttcgtgatgc tgcaactctg ctacgtctgg ggctggctta
    10981 cggccccggg gggatgtcat tacgtgaagt cactgcatgg gctcagctcc atgacgttgc
    11041 aacattatct gacgtggctc tcctgaagcg gctgcggaat gccgccgact ggtttggcat
    11101 acttgccgca caaacacttg ctgtacgcgc cgcagttacg ggttgtacaa gcggaaagag
    11161 attgcgtctt gtcgatggaa cagcaatcag tgcgcccggg ggcggcagcg ctgaatggcg
    11221 actacatatg ggatatgatc ctcatacctg tcagttcact gattttgagc ta
//
LOCUS       DAOFQD010000152        11115 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.152, whole genome shotgun sequence.
ACCESSION   DAOFQD010000152 DAOFQD010000000
VERSION     DAOFQD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.152"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1073
                     /gene="opgB"
                     /locus_tag="QBF46_004109"
     CDS             <1..1073
                     /gene="opgB"
                     /locus_tag="QBF46_004109"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDQ6676222.1"
                     /translation="KQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNYL
                     GLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGSH
                     FRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELAL
                     STDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRYK
                     ADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITAK
                     AYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGNI
                     LGHSPRKLGIGMVEIKVVEREG"
     gene            complement(1213..2235)
                     /gene="lgoD"
                     /locus_tag="QBF46_004110"
     CDS             complement(1213..2235)
                     /gene="lgoD"
                     /locus_tag="QBF46_004110"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDQ6676223.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            2374..3288
                     /locus_tag="QBF46_004111"
     CDS             2374..3288
                     /locus_tag="QBF46_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDQ6676224.1"
                     /translation="MSRSQNLRHNVINQVIDDMVRGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQH
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            3502..4863
                     /locus_tag="QBF46_004112"
     CDS             3502..4863
                     /locus_tag="QBF46_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6676225.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(4912..6576)
                     /gene="tsr"
                     /locus_tag="QBF46_004113"
     CDS             complement(4912..6576)
                     /gene="tsr"
                     /locus_tag="QBF46_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDQ6676226.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMESASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKNVTPATPRKMAVADSGENWETF"
     gene            6953..9103
                     /gene="btsT"
                     /locus_tag="QBF46_004114"
     CDS             6953..9103
                     /gene="btsT"
                     /locus_tag="QBF46_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDQ6676227.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            9153..9356
                     /locus_tag="QBF46_004115"
     CDS             9153..9356
                     /locus_tag="QBF46_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDQ6676228.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9367..10323
                     /gene="yjiA"
                     /locus_tag="QBF46_004116"
     CDS             9367..10323
                     /gene="yjiA"
                     /locus_tag="QBF46_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDQ6676229.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(10357..10659)
                     /locus_tag="QBF46_004117"
     CDS             complement(10357..10659)
                     /locus_tag="QBF46_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDQ6676230.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(10646..10933)
                     /locus_tag="QBF46_004118"
     CDS             complement(10646..10933)
                     /locus_tag="QBF46_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDQ6676231.1"
                     /translation="MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIEN
                     GEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIISARKADRKERNRYEHG"
BASE COUNT         2609 a         2979 c         2714 g         2813 t
ORIGIN      
        1 ataagcagga tcgcaataac ctgttttttg tcattcgtgg cgacaagccg cagcaagaga
       61 cgctggcagt gaagcgtaac acgatggata acggcgcgac ggtgctggac attctcggtg
      121 gcgataacta tctcggactt ggtcgtagca gtttatccgg gcagtcaatg tcggaaatct
      181 tcctcaatat caaagagaaa acattggcgt ggaagccgga tatcatccgc ctgtggaaat
      241 tccctaaaga gatgaaagag ttcaccatcg accagcagaa aaacatgatt gccttctcgg
      301 gtagccattt ccgtttgcca ctgctgttgc gggtttcaga caaacgcgtg gaaccgctgc
      361 cggaaagcga atactcagca ccgctgcgtt tccagctggc cgatttcgct ccacgcgaca
      421 atttcgtctg ggttgaccgt tgctataaga tggcacaact ctgggctccg gaactggcac
      481 tctccaccga ctggtgtgtc tcgcaagggc agcttggcgg tcagcaaatt gttcagcatg
      541 ttgacaaaac aacatggcag ggcaaaacgg catttaaaga tacggtcatc gacatggcgc
      601 gttataaagg caatgtcgat acgctgaaaa ttgttgataa cgatattcgc tacaaagccg
      661 acagtttcat cttcaacgtc gccggtgcgc cagaagaggt gaaacagttt agtgggattt
      721 cccgtccaga gtcgtggggc cgctggtcca acgcgcagct gggcgatgaa gtaaaaatcg
      781 agtacaagca tccgctgccg aagaaatttg acctggtgat taccgccaaa gcatacggca
      841 ataacgccag ccgtcctatt ccggtacgcg taggcaatga agaacaaact cttgtgctgg
      901 gcaatgaagt gaccaccacc acactgcatt tcgataaccc aaccgatgcc gacacactgg
      961 taattgtgcc gccggaacct gtctcaacca acgaagggaa tatcctcgga cactcgccgc
     1021 gtaagctcgg gatcggcatg gtggaaatta aagttgtgga acgtgaaggg taatttctga
     1081 ctgatgtgta ggtcgcataa ggtgcgacct acacatcttc tccggcctac aaaacatcat
     1141 tgagaaacag aaaaaaaaca gcgcccaaca cttccccgga cgctgcatgg caactttaat
     1201 aatctcgcct tatcagaaag taattacacc cttaatcaac tcacgattgt taatcacatc
     1261 gcgctcgtag gtttctgcca gcgtggcgaa cggatagcga tgggttaaca tcatgtcggc
     1321 agtgattttt ccttccgcca tcagtcgacc gactttagca aaatcttccg gcgtggcgtt
     1381 gcggctgccc atcatcgtcg tttctttttt atggaattcc ggatcggaga actgcaactc
     1441 acctttaaac aggccgacaa ataccaccgt gccgccgtga cgaatcaaat tcacggtgtt
     1501 attcatcgca tgttgattac ctgtcgcgtc gatcactttc tgcgccagcg aaccaccaaa
     1561 ctgcgcccgt agctgcgcgt caaaatcttc ggctgacggg tccagtaaag gtaattccag
     1621 acgcgttgcc acatgttcac ggcgcgccgg actggtatcc gccaccacca cctgtgcgcc
     1681 atcggcttta gcgattgccg ccgcgcccag accgattggc cccgccccga ccaccagcac
     1741 ctgctcgccg ggagcaatgg ctgcgcgacg caccgcatga gcgctaatag cgaaaggttc
     1801 aatcaatgct gccgcctgcg ggtcaatacc gtctgcgggc aaaatgttcg ccaccggcac
     1861 gctcaaatac tcactaaaac cgccatcctg atgcacgcca atgactgaaa ttttttcaca
     1921 gcaattggta cgcccgcttt tacacgccgg gcattgctga caggcaacat aagggatcac
     1981 agcaacttgc tgaccatttt taagatcagc aatattttta cccagcccaa caatctcccc
     2041 acatatttca tggcctaaaa cacgtggata actaaaaaat ggttgatttc caccccaggc
     2101 atgaatatcg gtaccacaaa tcccgacaga cttaattttt attaatgctt cattgtcacc
     2161 cggaataggt atctcgcgtt gtttccagac taattctttc ggctgctggc aaattaaaac
     2221 attcatcgta gacatgattc ggctctccat tttttgttgc cttagatatc aataaaaatg
     2281 aaaaatggca gcggaacgga aaatctgttt tgtgaaaaca cacgcataaa aatgttttaa
     2341 atcgggtttt aatggactca caaggaggaa aatatgagtc gttcacaaaa tttacgccac
     2401 aatgtgatta accaggtcat tgatgatatg gtgcggggtc atatcccttc cccacttcca
     2461 tcgcaaagtg cgctggcgga aatgtacaac atcagccgca ctacagtgcg tcatattctc
     2521 agccacttac gcgaatgcgg cgtcttaacg caggttggaa acgactatgt gattgcgcgt
     2581 aaacctgacc atgatgacgg tttcgcctgt actaccgctt caatgagtga acaaaacaaa
     2641 gtgtttgagc aggctttttt cacgatgata aaccagcacc agttacgccc tggggaaact
     2701 ttttctgaac tgcaactggc tcgggccgcg ggtgtcagcc cggttgtagt aagagaatat
     2761 cttttaaaat tcggccgtta taatcttatt caaagcgaaa agcgcggcca gtggagcatg
     2821 aagcaattcg atcaatccta cgccgagcag ctgtttgaac tacgcgaaat gctcgaaaca
     2881 cattcacttc agcactttct caacctgcct gatcatgatc cccgctggct gcaggcgaaa
     2941 accatgctcg aacgtcatcg gctactgcgt gacaacatcg gcaatagctt tcgcatgttc
     3001 tcgcaacttg acagagattt tcactcctta ctgctttcag ctgccgacaa tatctttttt
     3061 gatcaatctc ttgagatcat ctcagtcatc tttcattttc actatcaatg ggacgaaagc
     3121 gatcttaaac agcgcaatat cattgccgtc gatgagcata tgaccattct cagcgctctg
     3181 atttgccgca gtgaccttga tgccaccctt gcgctccgta accatttgaa ttcagctaaa
     3241 caatcaatga ttcgatccat taatgagaac acgcgttatg ctcattaagt acggattaaa
     3301 aacagcaaac tattagcggt gcacggtttt atcaaaagat aaataccggg aaattcccgc
     3361 ttacctatgc tcacaatcag tcgactaaaa atgtttttgt gagcggtagt aaagtcctaa
     3421 aactttaacc tgccgttaat gtcaaccttg cgtaatgcac aattacgtaa acaattaaaa
     3481 ttataaatac aggagtccgg cgtggaaaaa gaaaatatca ccctcgatcc gcgttcttca
     3541 tttactccat cttcgtcggc agatattccc gtgccaccag atggattagt tcaacgcagt
     3601 acccgaatta aacgcattca aaccaccgcc atgttgttat tattttttgc ggcggtaatc
     3661 aattatctcg accgcagttc gctgtcggta gcaaatttaa cgattcgtga agaattggga
     3721 ttaagtgcca ccgaaatcgg cgctttgctc tccgtgtttt cactcgctta cgggattgcg
     3781 caacttcctt gcggcccact attggatcgt aaaggcccac gcctgatgct gggactgggg
     3841 atgttcttct ggtcactgtt ccaggccatg tctggcatgg tgcacagctt tacgcagttc
     3901 gtgttggtgc gtatcggtat ggggattggt gaagcgccga tgaacccatg tggcgtaaaa
     3961 gtcattaacg actggttcaa catcaaagag cgcggacgcc cgatgggctt cttcaacgca
     4021 gcttctacca ttggcgttgc cgtaagccca ccgattctgg cggcgatgat gctggtgatg
     4081 ggctggcgtg ggatgtttat taccattggt gtgctgggga tttttctcgc cattggctgg
     4141 tatatgctct atcgcaaccg cgagcacgta gaactgactg ccgttgaaca agcttatctc
     4201 aatgcaggta gcgtcaatgc ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg
     4261 ttccgtaacc gcacaatgtg gggaatgatg ctcggattca gtggcatcaa ctacactgcg
     4321 tggctgtatc tggcctggct tcctggttac ctgcaaacag cctataacct ggatttaaaa
     4381 agcacagggt tgatggcggc tatccctttc ctgtttgggg ctgccgggat gctggtcaac
     4441 ggttacgtta ctgactggct ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc
     4501 tgcattattg ccgggatgtt ctgttctgcc gcctttacgc tggtcgtacc gcaagcgaca
     4561 acatccatga cggcggttct gctgattggc atggcactgt tctgtattca ctttgccgga
     4621 acatcctgct ggggcttgat ccacgtcgca gttgcttctc gcatgacggc gtcggtgggc
     4681 agtatccaga actttgccag cttcatctgc gcctcttttg cgccgatcat tactggtttt
     4741 attgttgata ccacccactc attccgtctg gcactaatca tctgcggttg cgtcaccgca
     4801 gcgggagcac tggcgtacat cttcctggtt cgtcagccga tcaacgaccc gcgtaaagat
     4861 taaactccct atgcccgata acattttgct tatcgggcat ttttatggcg attaaaacgt
     4921 ttcccagttc tccccgctat ctgccactgc cattttacgc ggcgtagctg gcgtcacgtt
     4981 ttttaccaca gccgatgttt cacgctgctg ttgctgctgt tgctgaatcc ggaacactgc
     5041 cacagcttcg gtcaggcgac tggcctgctc ttccagcgcg gcggcggcag cggcagattc
     5101 ttccaccagc gcagcgttct gttgagttac ccggtccatc tcagcaaccg ctaagccaac
     5161 ctgatcgata ccacggctct gctcatcaga agcagaagca atttcgccca taatgtccgt
     5221 cacgcgggtc acggcactga caatctccgc cattgtttcc cctgcgcttt cgaccagcgt
     5281 agagccaaca tctactttcc ccaccgagtc ttcaatcagg cttttaattt cacgagccgc
     5341 ctgagcgctg cgctgggcca gattacgcac ttcacccgcg accaccgcaa aaccgcgccc
     5401 ttgctcaccc gcgcgcgctg cttcaaccgc cgcgttcaaa gccagaatat tggtctggaa
     5461 ggcaatgccg tcaattacgc tgataatatc ggcgattttc tgcgaactgg tggagatatc
     5521 gcgcatagtc tgcaccacgt tatctaccac tttgccgccg cgttgcgccg tttcagaagc
     5581 acttaacgcc aggtggctgg cctggcgcgc attttcggcg ttctgtttca ccgttgcggt
     5641 cagttgctcc atgctggctg ccgtctcttc cagcgaagcg gcctgttgct cggtgcgcga
     5701 agagagatcg ttattgccgg tagcgatttc gctggcaccg ctatagatgg cattggcccc
     5761 gttgcgcaca tcaccgacgg tacgcatcag ctctccctgc atatggcgca aactctctgc
     5821 cagttgcccc atctcattag agccatccac ctcaatcggt ttcaccagat cgccgcctgc
     5881 aatatgacga atgctgtcaa tcaggcgatt cattggcgct accaacgagg ctttaatacc
     5941 gaaccagacg gcgaagatga ctgccagtac gacgatcatc acgcccacca gaatccacat
     6001 cgcctggctg taggaggcat tattatcgct gacggcgata tcatagagcc gatcgttttg
     6061 ctccatgtaa gccacatact gcttctcgaa accgtcctga tatccctggg tcggctgatc
     6121 aaagaactcg ttgattttgc ctgcacctaa cagttggatc agctccgcca gcgcattgtg
     6181 ataaatatcg taattacgtt tgatctctgc cgctgcggcg gtgctctgac gcgggtcacg
     6241 cggcaacgct tcgtaatccg cccagttttt ttccgcctgt ttcagcgaaa tactggcact
     6301 ctccatcagc tcagcaacgg ttgaaccgct accaatatta ttctgatcca tcatgtagcg
     6361 gatacccgcg cggttgaggg tgttacgcgt ctgcaacaac gcgacccagc tgccattcag
     6421 cgtggattgc tgctggcgaa tggtttgtaa aacagtgaaa ttttctttgt cattctttaa
     6481 ggcattaaag aacagaccgc ctgatgtcag ttgtaaaagg ccaaaaacgg ccaaaaccag
     6541 cagtaagctg gtcacaattt tgatacgttt taacatggtt tctctttcct gtggaccgat
     6601 gcaagatttt cggccaggaa aggaaaaact ttatgaatat cccgcggaga ttacgccgag
     6661 taattttatt cacactctga atttaaaaag ccattattta cataaattac tcacaatata
     6721 aatatgtgat atgaatcaca tatttatcgt cacttaaacg acgcctttgc cgctcaaccg
     6781 caaaactgac cgcttacatc cctaaaataa ccactcagtt atttacctta ctttacgcgc
     6841 gcgtaactct ggcaacatca ctacaggata gcggtcaatt tacctcctca aacgcaacgc
     6901 aaacctagaa cggcttcggc caactattaa tcaatacatg ccaggtttta ctatggatac
     6961 taaaaagata ttcaagcaca taccctgggt gattctcgga atcatcggtg cattctgcct
     7021 cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg tggtcgcctc
     7081 tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg tacatcgccc agaaggtgat
     7141 gaaactcgac cccacgcgcg cgacgcctgc ggttattaac aacgacggtc tgaactacgt
     7201 tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg gtgctggtcc
     7261 gctggtgggt ccggttctcg ccgcgcagat gggctacctg cctggcacgc tgtggctgct
     7321 ggcgggggtc gtgctggccg gtgcggttca ggactttatg gtgctgttta tctcctctcg
     7381 ccgtaatggc gcatctcttg gtgagatgat caaagaagag atgggaccag taccggggac
     7441 tatcgcgctg tttggctgtt tcttaatcat gatcatcatc ctcgccgtcc tggcgctgat
     7501 tgtggttaaa gccctggccg aaagtccgtg gggtgtcttc accgtttgct caaccgtacc
     7561 gattgcgctg tttatgggta tctacatgcg ctttatccgt ccggggcgtg tgggtgaagt
     7621 ctctgtcatt ggtatcgtgc tgctggttgc ctctatctac ttcggtggcg tgattgctca
     7681 cgatccgtac tggggtccgg cactgacctt taaagacacc accattacct tcgcgctgat
     7741 tggctatgcg tttgtttccg cactgctgcc ggtgtggctg atcctcgcac cgcgcgacta
     7801 tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg gcgctgggta tcgtggtgct
     7861 gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt gacggtactg gcccgctgtg
     7921 gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc tgtggtgcgg tatctggctt
     7981 ccacgcgctg atctcttccg gtacgacgcc gaaactgctg gctaacgaaa ccgacgcgcg
     8041 tttcattggc tacggcgcaa tgctgatgga gtccttcgtg gcgattatgg cgctggttgc
     8101 tgcgtccatc atcgaaccgg gtctttactt cgcgatgaat accccgcctg cgggccttgg
     8161 catcaccatg cctaacctgc atgaaatggg tggcgagaac gcgccgatca tcatggcgca
     8221 gctgaaagac gttaccgcac acgcggcggc gaccgtcagc tcctggggct tcgtgatttc
     8281 gccagagcag atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc tgaaccgtgc
     8341 aggtggtgcg ccgacgctgg cggtaggtat cgctcatgtg ttccacaaag tgctgccgat
     8401 ggctgacatg ggcttctggt atcacttcgg tattctgttc gaagccctgt tcatcctgac
     8461 cgcgctggat gcgggtaccc gttctggccg ctttatgctg caagacctgc tgggtaactt
     8521 catcccgttc ctgaaaaaaa ccgactctct ggttgccggt atcatcggta ctgcgggctg
     8581 tgtgggtctg tggggctacc tgctgtatca gggcgtggtc gatccgctgg gcggcgttaa
     8641 gagcctgtgg ccgctgttcg gtatctccaa ccagatgctg gcagccgtag cgctggtact
     8701 gggcaccgtt gtgctgatta agatgaagcg cacccaatac atctgggtaa ctgttgttcc
     8761 ggctgtatgg ctgcttatct gcaccacctg ggcgctgggt ctgaaactgt tcagcaccaa
     8821 cccgcagatg gaaggcttct tctacatggc aagccagtac aaagagaaga ttgctaacgg
     8881 tactgacctg acggcgcagc agattgccaa catgaaccac atcgttgtga acaactacac
     8941 caacgcgggc ctgagtattc tgttcctgat tgtggtgtac agcatcatct tctacggttt
     9001 caaaacctgg cttgcggtgc gtaacagcga caaacgtact gacaaagaaa caccgtacgt
     9061 tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac taaccgtgtt tagccccgct
     9121 tcggcggggc tttgttctta tcagagtgaa ctatgtttgg taacttagga caggcaaaaa
     9181 aatatctcgg ccaggcggca aagatgttga ttggcattcc agactacgac aactacgtcg
     9241 agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa ttcttccgcg
     9301 aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg tatgcgctgt tgttaatgga
     9361 gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc gggaaaaacc
     9421 accctgctgc gccatattct taacgaacaa cacggctaca agattgccgt gattgaaaac
     9481 gaattcggcg aagtctccgt tgatgatcaa ctaattggcg accgcgccac gcagatcaaa
     9541 acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggaaga cgcactactc
     9601 gacctgctgg ataatctcga caagggcaat attcagtttg accgtctggt cattgaatgc
     9661 accggcatgg ccgatcccgg cccgattatt cagacctttt tctcccatga gattttatgc
     9721 cagcgttacc tgctggacgg tgtgattgcg ctggtggatg cggtacatgc cgacgagcag
     9781 atgaaccagt tcaccatcgc ccagtcccag gttggctacg ccgaccgtat tctgctgacc
     9841 aaaaccgacg tcgcaggcga agcagaaaaa ctgcgtgaac gcctggcgcg catcaacgcc
     9901 cgtgcaccgg tctacaccgt cacccacggc gacatcgacc tgggcctgct gttcaacacc
     9961 aacggtttta tgctggaaga gaacgtcgtc agcaccaaac cgcgtttcca ctttatcgcg
    10021 gataaacaaa acgatatttc gtcgattgtg gtggaactgg attacccggt agatatcagc
    10081 gaagtttccc gggtgatgga aaacctgctt ctggaatcgg cagataaact gctgcgttac
    10141 aaagggatgc tgtggattga cggcgaacct aaccgcctgc tgttccaggg cgtccagcgc
    10201 ctctacagcg ccgactggga ccgcccgtgg ggcgatgaaa aaccgcatag cacaatggtg
    10261 tttatcggga ttcagttgcc ggaggaagag attcgggcag cgtttgcggg gttgaggaag
    10321 taattttgga tgatggccgc catgtggcgg cctgaattat tttttgttct gatcgcgcag
    10381 catcgcttca cgtaaaattg cgttcaggcg agtttgatag cctttacctg gtcgcttcaa
    10441 ccattccatc acatcagcat caatacgcac cgaagcctga gttttcaatg gccgataaaa
    10501 cttaccgcgt tcggcgttag accattgctc atcgctggaa ggtgggatat cactgtaatc
    10561 aatatcttcg tcggatttat tcgtcagcgc cttcagttcg gcctcatgct gcacatcgag
    10621 tgtagaggag gtccctcgtt tatgtttaac catgctcata gcgattcctc tctttacgat
    10681 ctgcttttcg tgcactgatg attcgaataa tttcatttcc gctttcgaaa cggatggtat
    10741 gcgcaaccag aataaccacg atgccgtaaa ccaggccgat tgtctgccag cgatactcac
    10801 cgttttctat gcgttcttgc tgtgacaaat gttgtggatc atcaaacaat aacaccgcat
    10861 cttcaaagcg gatgccatgt ttcacccgat tacttttggc cttgttttca tcccattcaa
    10921 actccattgg cataattgcc atccctaatt gtatatacaa aaatgtatct acaattattc
    10981 tgcaattata gcgttgatgt tggaaatagc atcacgtttc gaataatcaa ccatgaaatt
    11041 gacaatgagt aagttggact tgattcatcc aatagaattt ctcgcctgac atggcacact
    11101 aaacacctcg ctgtc
//
LOCUS       DAOFQD010000153        11104 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.153, whole genome shotgun sequence.
ACCESSION   DAOFQD010000153 DAOFQD010000000
VERSION     DAOFQD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.153"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="QBF46_004119"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="QBF46_004119"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDQ6676232.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="QBF46_004120"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="QBF46_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDQ6676233.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3745..4164)
                     /gene="atpC"
                     /locus_tag="QBF46_004121"
     CDS             complement(3745..4164)
                     /gene="atpC"
                     /locus_tag="QBF46_004121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDQ6676234.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4185..5567)
                     /gene="atpD"
                     /locus_tag="QBF46_004122"
     CDS             complement(4185..5567)
                     /gene="atpD"
                     /locus_tag="QBF46_004122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDQ6676235.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNCNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5594..6457)
                     /gene="atpG"
                     /locus_tag="QBF46_004123"
     CDS             complement(5594..6457)
                     /gene="atpG"
                     /locus_tag="QBF46_004123"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDQ6676236.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6508..8049)
                     /gene="atpA"
                     /locus_tag="QBF46_004124"
     CDS             complement(6508..8049)
                     /gene="atpA"
                     /locus_tag="QBF46_004124"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDQ6676237.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8062..8595)
                     /gene="atpH"
                     /locus_tag="QBF46_004125"
     CDS             complement(8062..8595)
                     /gene="atpH"
                     /locus_tag="QBF46_004125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDQ6676238.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLVFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8610..9080)
                     /gene="atpF"
                     /locus_tag="QBF46_004126"
     CDS             complement(8610..9080)
                     /gene="atpF"
                     /locus_tag="QBF46_004126"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDQ6676239.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9142..9381)
                     /gene="atpE"
                     /locus_tag="QBF46_004127"
     CDS             complement(9142..9381)
                     /gene="atpE"
                     /locus_tag="QBF46_004127"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDQ6676240.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9428..10243)
                     /gene="atpB"
                     /locus_tag="QBF46_004128"
     CDS             complement(9428..10243)
                     /gene="atpB"
                     /locus_tag="QBF46_004128"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDQ6676241.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10252..10632)
                     /gene="atpI"
                     /locus_tag="QBF46_004129"
     CDS             complement(10252..10632)
                     /gene="atpI"
                     /locus_tag="QBF46_004129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDQ6676242.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2650 a         3022 c         2749 g         2683 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgacg tggtaagcca gcagctgcag
      121 cggaacggtg tagaagattg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca actgaccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacaacaata accggcatat cggcatcaat tagcgccagc ggaccgtgtt tcagttcgcc
      361 agcagcgtag gcttcagcgt gaatgtaaga gatctctttc aacttcaatg cgccttccag
      421 cgcgattggg tactgatcgc cacggcccag gaacagcgcg tgatgtttgt cagagaaatc
      481 ttctgccagc gcttcaatgc gtttgtcctg agacagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacga tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgggtg gtgaaggctt tagtggatgc
      661 cacgccaatt tccgtaccgg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggccagcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttgc gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg tccggtaagg gtgtttttga tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaatg cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatcc ccaggccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccac gcagctgcgg gatagcacgc agaacggcct cacgcagagt
     1441 cccgccttgt ttcagctccc aattcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttagctc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcattca cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccagtg ccgccatgca gaggatgttc
     1681 ttccgctgcc tgtgccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttctgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccatgt gaccgggtta gccggccaga atcacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accaacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgacca gctttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc gacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgggcaaacg ggccaatggt acaggccgct gccagattcg catcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagttagcgt accacgcaga tcaaaacgcg ctggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactgataaa cacgctccag acgggagagt
     2701 tgcaggcggt tattcacgcc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttggc aatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccgttttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcagacgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagttc
     3181 aggttgtcgt ctttcagcgc ctgttttagc agatcgccgc cgtgaccgta caccaggtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagcgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttaatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacaggaa aacagggggg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg ggtcacacgc gcaaaaaaaa gccagcctgt ttccagactg
     3721 gcttttgtgc ttttcaagcc ggtgttacat cgcttttttg gtcaactcga taacgcgcag
     3781 ctgcgcgatc gctttggcca gttccgcaga cgcctgagcg taatctacgt cgccgtgaga
     3841 gctgctaatg tgctcttcag ccttacgttt cgcttccatg gctcgcgctt cgtcgagatc
     3901 ctggccgcga attgcggtgt cggccagaac ggtcacgttg ccaggctgca cttcaagaat
     3961 gccgccagac agatagataa actcttcgtg accgtgctgt ttcacgatgc gaatcatacc
     4021 aggcttaatg gcggtgagca gcggtgcgtg gccagggtag atccccagtt caccttcgct
     4081 acccgttacc tggattttct cgaccagacc agagaacatt tgttgctctg cgctgacgac
     4141 gtccaggtgg taagtcattg ccatatcacc ctccgattaa ggcgttaaag ttttttggct
     4201 ttttccacag cttcttcgat ggaaccgacc atgtagaacg cctgctccgg caggtgatcg
     4261 tattcgcctt ccatgatgcc tttaaagcca cggatggtgt ctttcaggga gacgtattta
     4321 cccggagaac cggtgaatac ttctgccacg aagaacggct gggacaggaa gcgctggatc
     4381 ttacgagcac gcgctaccac cagtttgtct tcttcagaca gttcatccat acccaggatg
     4441 gcgatgatgt ctttcagttc ctgataacgt tgcaggatgg actgaacgcc acgcgcggtg
     4501 tcgtagtgtt cctgaccaac caccagcggg tccagctgac ggctggtgga gtccagcggg
     4561 tcaacggccg ggtagatacc cagagacgcg atctgacggc tcagtaccac ggttgcgtca
     4621 aggtgcgcaa aggtggttgc cggagacggg tcagtcaagt catccgcagg tacgtatact
     4681 gcctgtacgg aggtgataga accggttttg gtggaggtga tacgctcctg cagaacgccc
     4741 atctcttccg ccagggtcgg ctgataacct accgctgaag gcatacggcc cagcagtgcg
     4801 gatacttccg taccggccag ggtgtaacga tagatgttgt caacgaacag cagaacgtca
     4861 cgaccttcgt cacggaattt ctcagccatg gtcagaccgg tcagagcaac gcgcagacgg
     4921 tttcccggcg gctcgttcat ctggccatac accagggata ctttgtcgat aacgttggag
     4981 tcggtcattt cgtggtagaa gtcgttaccc tcacgagtac gttcacctac gcccgcaaac
     5041 acagagtaac cggagtgctc gatcgcgatg ttacgaatga gctccatcat gtttacggtt
     5101 ttacctacac ccgcaccacc gaacagacca actttaccgc ccttagcgaa cggacacatc
     5161 aggtcgataa ctttgatacc ggtttccagc agttcctgag agtttgacag ctcttcgtag
     5221 gaaggtgctg cgcggtgaat cgcccaacgc tcttcttcac cgatctcgcc tttcatgtcg
     5281 accggttcac ccagtacgtt catgatacgg cccagagtcg ctttacctac cgggacttcg
     5341 atcgggtgtt cgaggtcttt tacatccaga ccgcgacgca gaccgtcgga ggaacccatt
     5401 gcgatggtac gcacgatacc gccgccgagc tgctgctgaa cttccagcac cagacgctca
     5461 ttacaatttt gcacctcaag agcatcgtac acgcgcggta cggcatcctg agggaattcg
     5521 acgtcaacta cggcgccgat tacctggaca atctttccag tagccatctt aaatcctcta
     5581 cgaaataacc tgtttaaacc gcggcggccc ccgagacgat ctcggtgagt tcctgagtaa
     5641 tgctggcctg acgagctttg ttgtatacca actgcagctc tttaatcagg ctgccgccat
     5701 tgtcggtcgc ggctttcatc gccaccatac gggcggcctg ctcgctggcc aggttttcaa
     5761 ccacgccctg ataaacctga gattcgacat aacgacgcag cagggtatcc agcaacgcct
     5821 tcggatcggg ttcgtacagg taatcccagg atttatgttt cagatcatca tcatctgatg
     5881 ccggtaacgg cagcagctgg ctgatggtcg gaacctgaga catggtgtta ataaatttgt
     5941 tgctgacaat gtaaagcttg tccagacggc cttcgtcgta ggcctgcaac atcactttta
     6001 ccggaccgat cagttcggac agggaagggt tatcccccat gccggtgacc tgggcaacaa
     6061 cattgccgcc cacggagttg aagaacgaca cgcctttcga gccgatcatt gcgaggtcgc
     6121 attgaacgcc tttgtcggtc caggtcttca tttccgccag cagttttttg aacaggttaa
     6181 tgttcaaacc accgcacaaa ccacggtcgg tcgacaccac caggtagccc acgcgtttaa
     6241 cgtcgcggtc ttccaggtaa gggtgcttgt attccagatt accgtgtgca aggtgaccaa
     6301 tcactttgcg catggtttct gcataaggac ggctggccgc catgcgatcc tgcgatttac
     6361 gcattttgga agcggcgacc atctccatcg ctttagtgat cttttgcgtg ttctggacgc
     6421 ttgcgatctt actacgtatc tcttttgcgc cggccatgag cttctcctca atgccttgcg
     6481 gcctgcccta aggcaagccg ccagacgtta ccaggattgg gttgctttga aggaatcgag
     6541 gatgcctttc agcttgcctt cgatttcgtc gttgtagcca ccggtctggt tgatctcttg
     6601 catcaacgga gcgtgatcac ggtcgacgta agccagcaga gcggcttcga agctgccaat
     6661 tttcgacagt tcaacatccg ccaggtaacc acgttctgct gcgaacagaa ccagagactg
     6721 ctgcgcaacg gacatcggcg catactgttt ctgtttcagc agttcggtca ctttctgacc
     6781 gtggtcaagc tgcttacgtg ttgcatcgtc aaggtcggat gcaaactgag agaacgctgc
     6841 cagttcacga tactgtgcca gagcggtacg gataccaccg gacagttttt tcatgatctt
     6901 ggtctgtgct gcaccaccaa cacgggatac ggaaataccc gggttaaccg caggacgaat
     6961 accggcgttg aacaggttgg tttccaggaa gatctgacca tcggtaatgg agattacgtt
     7021 ggtcggaacg aacgcagaaa cgtcacccgc ctgagtttcg ataatcggca gtgcggtcag
     7081 agaaccggtt ttccctttca cttcaccttt ggtgaaggct tcaacgtatt cggcgttaac
     7141 acgtgcagca cgctccagca gacgagagtg gaggtagaaa acgtcgcccg ggaatgcttc
     7201 acgtcctggc ggacgacgga gcagcaggga gatctgacgg taagcaacag cctgtttaga
     7261 caggtcatcg taaatgatca gcgcatcttc accgcggtca cggaagtatt cgcccattgc
     7321 gcaaccggca tacggtgcca ggtattgcag tgcagcggat tcagacgcgg ttgctaccac
     7381 aacgatggtg ttagccagtg cgccgtgctc ttccagttta cgtaccacgt tagaaatggt
     7441 ggacgctttc tggccgatag cgacatagat acatttgata ccggaatcgc gctggttgat
     7501 gatggcatcg atagccagtg cggttttacc tgtctgacgg tcaccgatga tcaattcacg
     7561 ctgaccacga ccgattggga tcatggagtc aacggcttta taaccggtct gtaccggctg
     7621 atctacggac tgacgttcga taacgcccgg agcgattgct tctacagcag agaagccgtc
     7681 gtgatccagc ggacctttac cgtcgattgg tgcacccaga gtgttaacca cacggcccag
     7741 caggccacgg ccaaccggaa cttccaggat acggccagta cacttaactt tcatgccttc
     7801 ggcaaggtca gcgtacggac ccataacaac cgcacctaca gagtcgcgct cgaggttcag
     7861 tgcgatagcg taacggttac ccggcaggga gatcatttca ccctgcatac aatcggccag
     7921 gccgtgaatg cggataacac cgtcacttac agaaacaata gtaccttcgt tgtgagcttc
     7981 actcacaaca ttgaactgag caatgcgctg cttgatcagt tcgctgattt cggtggaatt
     8041 cagttgcatg ctccagtccc cttaagactg caagacgtct gcaaggcgct caagacgacc
     8101 gcgtacgctg ccatcaatga ccatatcacc cgctcggatg ataacgcctg ccattacaga
     8161 cttatcgatt ttgcaattca gcttaacttt gcgtgacaga cgtttttcca tcgcagcaga
     8221 aattttcgcg agctgttgtt cactcagtgc ggcagcggaa atgacgtcta cctcagcggt
     8281 agcctcactc acggcacgaa ggtgaataaa ctgctccaga acatccggga gcgcgttaag
     8341 acgaccattt tcagccatta cccgaatcag gttctgaccg ttttcgtcca gttgctcacc
     8401 acaaactgcg ataaacgact cggcgagcgt ttctggcgca agcgcgccag agagaagctc
     8461 tgccatttgt tcgtttttgg ttacctcggc ggcaaacacc agcatgtcct gccagcgttc
     8521 tacactttgg tgttcgacgg caaagtcaaa agctgctttg gcgtaggggc gagctaccgt
     8581 aataaattca gacatcagcc cctccctcct tacagttcag cgacaagttt atccacgatg
     8641 tcgctgttag cagcttcatc cacggaacgt tcgatgatct tctcggcgcc agcaacagcc
     8701 aggatagcaa cttgcttacg cagctcttca cgggcacgtt tacgctcggc ttcaatttct
     8761 gcctgcgcct gggccacgat tttagtacgt tcctgttctg cctcagcttt cgcttcgtcc
     8821 agaatctgcg agcggcgttt gttcgcctgc tcgatgatta cctgggcttc cgctttcgct
     8881 tttttcagct ggtcggtcgc gctggccttt gcaaggtcaa ggtccttatg tgctcgttct
     8941 gcggaagcaa ggccgtcagc aatttctttt tgacgttttt cgatggctgc cattaatggc
     9001 ggccatacgt acttcatgca gaacagaacg aacaggacaa acgcgatggc ctggccgagg
     9061 attgttgcgt taagattcac agcacaatgc ctctatttag ttaacgttct gatattgctc
     9121 tttaaataaa agcaacgctt actacgcgac agcgaacatc acgtacagac ccagacctac
     9181 agcgatcatc gggatagcat ccaccagacc cataacgata aagaactgag tacgcagcag
     9241 aggaatcaga tcaggttgac gcgctgcgcc ttccaggaat ttacccccga ggatgccgat
     9301 accgatcgca gcaccgattg ccgccagacc catcatcaca gcggcagcca tgtacagcag
     9361 atccatattc aggttttcca tgacagtctc cagtttgttt cagttaaaac gtagtagtgt
     9421 tggtaaatta atgttcttca gacgccatcg acagatagac gatcgtcaga accatgaaga
     9481 tgaaggcttg cagcgtaatg atcaggatgt ggaaaatggc ccacggcaca ttcaggatcc
     9541 actgtgacca ccacggcaac agaccagcaa tcagaatgaa aatcagctca ccggcataca
     9601 tgttaccgaa cagtcgcaaa ccaagtgaaa ctggtttgga cagcaggctt accccttcaa
     9661 ggattaagtt gacaggaatg aacgcccagt gattgaacgg ctgcagcgtc aactctttcg
     9721 tgaagccgcc gatgcctttc attttgatgc tgtagaacag aatcaggata aatacgccca
     9781 atgccataga cagcgttacg ttcacgtccg cagacggaac cacacgcagt gcaggcagac
     9841 ccagtacatg ttcagcaatg tacggcagca ggtcgatagg cagtaaatcc atcaggttca
     9901 tcaggaatac ccagacgaag atcgtcaggg ccagcggagc aatcagcttg cttttgccat
     9961 ggtacatgtc tttcacgcta ccattaacaa agccgatcac cagctcaatc gcggtctgaa
    10021 acttacctgg cacaccgctg gtcgcctttt tggctacgct acggaataaa accaggaaca
    10081 acagacccag caccaccgag aagaacatgg agtcaatatt gattgtccag aaggtggctg
    10141 gggggttttg tggatccacc agcgagaatg tacgcaggtc cagctgaagg ttattcaggt
    10201 ggtgtcctat gtaatcctgc ggcgtcatat tttctgaagc catgatgcct tttacccttt
    10261 gttgttaatt acagccggtg ccagtatctg aaccaccagc accaaaaccc acgtaacgat
    10321 cagcggcaag aataccgcct ttaaaaccgc caacgccacc accagtaaca ccaacatcgc
    10381 cagaactttg aaagcttcgc caaatgcgaa tgtccaggcc acccggcctt tcgctggtgt
    10441 atgcgcctgg tgacgccagg caaatatcat aaacaaaacg ttaggcagaa agactgccag
    10501 gcccccgctt attgcagaga cgccccagaa ggggtctttg aggctgaaca gcaatccact
    10561 tgctatcacc accagtaact gaacgagcag aagcttccga gcaacgtttc gactcacgag
    10621 cgacacagac atcacgtttt tcactcctgc tcccttcgag gtatgccgcg tgtcgtataa
    10681 aactttcttt aaggcttaga gtcaagcatc aaaaagcggt caaattatac ggtgcgcccc
    10741 cgtgatttca aacaataagt agccaaaagg tgaataaatg tttaaatatt tttccagtgc
    10801 atacaattgc gacttttctg ctaaccctgt tcgatcatga aaaactgtaa ataacgcgta
    10861 aacactggtg ataaagcgtg cttcagatca catattgcgc atgttcgcgc acagcatatt
    10921 tatttacttg gcaaatgatg cctttgcaag tttatgatat ttcagtctaa aaacagatac
    10981 tgttttaata aatgacattt acacaacaaa aaccacccat tgacattttt aataatgttt
    11041 taacagccaa tgatggttct tagcgccgat ttttagcaga ctgatatttt cactaatgac
    11101 ttat
//
LOCUS       DAOFQD010000154        11081 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.154, whole genome shotgun sequence.
ACCESSION   DAOFQD010000154 DAOFQD010000000
VERSION     DAOFQD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.154"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="QBF46_004130"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="QBF46_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ6676243.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="QBF46_004131"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="QBF46_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDQ6676244.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="QBF46_004132"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="QBF46_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDQ6676245.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="QBF46_004133"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="QBF46_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDQ6676246.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="QBF46_004134"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="QBF46_004134"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDQ6676247.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIILIDYTDFVRLTVKHPSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="QBF46_004135"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="QBF46_004135"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDQ6676248.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="QBF46_004136"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="QBF46_004136"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDQ6676249.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIADGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRAWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /locus_tag="QBF46_004137"
     CDS             complement(4523..5245)
                     /locus_tag="QBF46_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6676250.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="QBF46_004138"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="QBF46_004138"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDQ6676251.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATAADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /locus_tag="QBF46_004139"
     CDS             6445..6663
                     /locus_tag="QBF46_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDQ6676252.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="QBF46_004140"
     CDS             complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="QBF46_004140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDQ6676253.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7397..7597)
                     /locus_tag="QBF46_004141"
     CDS             complement(7397..7597)
                     /locus_tag="QBF46_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDQ6676254.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="QBF46_004142"
     CDS             complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="QBF46_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDQ6676255.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="QBF46_004143"
     CDS             complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="QBF46_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDQ6676256.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10094..>11081
                     /locus_tag="QBF46_004144"
     CDS             10094..>11081
                     /locus_tag="QBF46_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDQ6676257.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILD"
BASE COUNT         2718 a         3042 c         2670 g         2651 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg cgtatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgttcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggggtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgagaatga tgtcgttcga aatttgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt accgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtctc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgtcag ccagggtgag cgatgtaatg tgtgcagcat aaacgcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag acccaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tggcagcagc tgaaaaacgc catcagctat
     4021 aaagaagaca gccagatcgt cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccggcctgcg gctgtaccat gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgccccaag tccgcttaag
     4201 gtaaatccct gctgaaggtt tgacgaagcc agccccagtc ttccggcctc cgtttcatca
     4261 acaacgccac ggcgtaatgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcagttgtt gccaggcccg tacgaggtca aattcgtcac ttgccggaga ggtcaattgg
     4381 ttagcgttat agaccccttc ccgatagaaa aagacgctgc ttaactcatg gccatctgct
     4441 atcagcgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg gaaatcaccg ctcttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcatcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacattg cgatcggcat tcacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaggtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacatccagc agctctacgt caaacaccag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc aacccgggat aacaccgtcc
     5641 agacggaaag aaagcggttc accacgggtg taagagttgt cgaactcttt accgtcgatc
     5701 agcgtacctt tgtagttcac tacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgta gagtctgttc gatctcttgg
     5941 tcggagagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct tttagagagt tttccatgta acgacccagc
     6061 gaggcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgctgc tttgctgtca
     6121 gcagctgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagcag cgttacttta aacagtgatt tcatccatat ctccaggatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaagcacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtgacc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagtta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgaag aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca ctttttcggt attagtggca
     6721 accgcaaccg ccgttgcctt taccgccaca gcagccttcg ccaccgtgtt catgaccgtg
     6781 atcgtggcca tgaccgccgc agcaaccgtc gtggtcgtga tcgtggtggt gatcgtgcgc
     6841 gccgtgaacg tgaccatgag ccagttcttc ttcagtcgct tcgcgaatcg ccacaacttc
     6901 aacgttgaat ttcaggttct gaccggccag catgtggtta ccatcaacca cgacgtgatc
     6961 gtcttcaacc gcagtgattt caaccggtac cggaccctgg tcggtttcag ccaggaaacg
     7021 catacctacc tgcagttcat caacgcccat aaatacgtct ttaggaacac gttgcaccag
     7081 gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc
     7141 aacttcatga ccttccagcg ccgtttccag gccagagatc agggaaccgt gaccatgcag
     7201 gtagtccagc ggcgcactca ccggagactc atcaaccaac acaccgtctt ctgtacgtac
     7261 ctgataggcc aggctgacca ccaggtcttt tgctactttc atgatatctc ctgagcatgg
     7321 gaagaatagt ggcgcagatt gtagcggaat tctgcagccg tgtactcact agcttaaaaa
     7381 aactcggcgc atatcgctag tccggatgaa aaatcccgat cacttgctca tctttgcgaa
     7441 cgtgatcgcg ggcttctttg tctgcttctc gcatctgatg tccgcactta acacattcaa
     7501 caatatcaat attattttcg cgccacatcg ccattgaatc ctgcgcctga caggccgggc
     7561 attttgcgcc cgcaataaaa cgttttcgga ttgccatctt taccctctac tcaaattcat
     7621 cccagccgtc cagctggcgt cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt
     7681 cgcgtcgggc ttccctggcg cgagaaattt gtacggtatc ggcatgcatt gggatgagct
     7741 ctcgcagcat ccgcatatcc aggcggcgaa aatgcagttg cgctcgctgt gcctgatgcg
     7801 gatgcatgcc aagcgtgacc agcgtcttgc gccccagctc taacgcactg gagaatgttt
     7861 cacgggaaaa ctgcgtcacc cctgcctgta ataactcatg cgcttccaca cgtccgcgcg
     7921 ctcgcgcaag aatatgcaaa tgcggaaagt gctgttggca tatttccacc agcttcatgg
     7981 tgtcttccgg ctcgttacag gtaatgacga tagactcagc ggcctctgca cccgcagaac
     8041 gtaaaagatc gacctgcgtg gcgtcgccgt aataaacttt gtagccgtat ttacgcatca
     8101 ggttaacggc gctgatatcc cgctccagca cggtaatgcg cattttattt gccatcagca
     8161 aacgaccaat cacctgacca aaacgcccga agcccacgac aatgacctgg ggtttatcat
     8221 cgttgaccca cggtttttcg tcttcttctt ccggtccgtt aaactggcgg gatagccatt
     8281 tatccaccag cttcatcagc aacggcgtgg tcatcatgga aagcgtcacc gtcaccagca
     8341 acaacgccat ctggtcgccc tggaataagc gttgtgaaga agcggtagaa aagaggacaa
     8401 aggcaaactc accaccctga ctcaacacgc cagcaaactg catccgctct gagctacgca
     8461 cgccatacaa tcgcgccagc agatacagca cgagaatttt caccgccacc agcacaacca
     8521 cgcttatcac tacccacaac agatgggtat aaagcacccc gaggttgagt gacatgccga
     8581 cagagataaa gaacaaaccg agcagcaagc ctttgaaggg atcgatagcc gtttccagtt
     8641 catggcgata ttcactttcc gccagcagca cgcccgcaat aaatgtaccg agcgccatcg
     8701 acagccccag cgcatccata aacaatgcgg aacccaacac cagcagcagc gtcgcggcgg
     8761 tgaacacttc ccgcacgcca gaagctgcaa taaagcggaa taccggacgc agtaaatagc
     8821 gcccaccaat cagcatgccg acaaacgcca gcaccttcat gccgatcttc atccagtcga
     8881 aatgttcgtc tgccgacccc gccaacaacg gcactaacgc cagtgctggg attactgcca
     8941 gatcctgaaa cagcagaacc gaaaacccga gctggccgga ttcgctgcga ttcatccctt
     9001 tctcacgcat caattgcaac gccattgcag ttgaagacat cgcaaggcca atgccaccga
     9061 ccaccgccgc ctgccaggcg aaatccgtca gcatcaataa tcccgccagc aacgccgcgc
     9121 ttaacagcac ctgtgccgcg cctacgccaa aaatcgaacg ccgcagttgc caaagtttgg
     9181 agggattcaa ctcaaggccg atgataaaca tcaggaatac cacgccgagt tccgaaaagt
     9241 ggaggatctc atcgacgtcg ctaataaacc ccagccccca cgggccaatt gcaatccctg
     9301 ccagcaaata tcccaacaca gcgccaatac ccagccgcga tgccagcggc accgcagcca
     9361 ccgccgcgaa gagaaacagc actcctgcga gtaaaaaatc ggaaccttcc atcagcggcc
     9421 tcctggagac agcggatttg ccagccagtc accgtaggct ctggcgtggc tcgccagctc
     9481 ctgtgcgctt tgccgtctcg cccagtaaat aatgatggga cttaaccaat gcatccggca
     9541 catgcccgcc gccagttcaa aggggcgcag cacatcgctc atcgggtagc gattcagcgc
     9601 gtcataacgg taagcacttt ccggctcgcc ggtggtaatc acgctacgcc agtactttcc
     9661 cgccagttgg tttcctcccg gcccgctggc aaaaccacga cttaataccc ggtccagcca
     9721 ctctttcagt agcgccgggc agctataggt ataaagagga tgttgaaaga caatcacctc
     9781 gtgctcgcgc agtaatgcct gctcacgggg gatatcaata aaaaaatcgg gatagtgcgc
     9841 gtaaaggtcg tgcacggtaa cattgctgag ctgcgtggcc ggtttaagca gtacccggtt
     9901 tgccaccgag tcctgagatt ccggatgggc atacagcagc aaaactttcg ctggctgaga
     9961 catcatcccc ctcccggtat ggtttttgtg tatagtcgct gttttgggct accattgcgc
    10021 ccggtgcggc agctcgccca tacattacat tatcataatg ataagttaac atagtctgaa
    10081 catacggcac cttatgattg ttttctcctc gttacaaatt cgtcgcggcg tgcgcgtcct
    10141 gctggataat gccaccgcca ccatcaaccc tgggcagaaa gtcggcctgg tgggtaaaaa
    10201 cggctgtggt aaatctactc tgctggcatt gctgaaaaat gaaatcagcg ccgacggcgg
    10261 cagctacacc tttccgggaa gctggcaact ggcgtgggtg aatcaggaaa cgccggcgtt
    10321 accgcaagcg gcgctggaat atgtcattga cggcgaccgt gaatatcgtc aactagaagc
    10381 gcagctacac gacgccaacg aacgtaacga cgggcacgcc attgcgacca ttcatggcaa
    10441 gctggatgct attgacgcat ggagtattcg ctcccgtgct gccagcctgc tgcacggcct
    10501 cggtttcagc aatgaacaac tggagcgccc ggtaagtgat ttttccgggg gctggcgtat
    10561 gcgtcttaac cttgcccagg cgctgatttg ccgttcagac ttgctgctgc tcgacgaacc
    10621 gactaaccac ctcgatctcg atgccgttat ctggctggaa aaatggctga agagctatca
    10681 gggcacgctg atcctgatct ctcacgaccg cgacttcctc gatccgatcg tcgataaaat
    10741 tattcatatc gaacaacaaa gcatgttcga gtacaccggc aactacagtt cgtttgaagt
    10801 acagcgcgcc acccgtctgg cgcagcaaca agcgatgtac gaaagccagc aggaacgcgt
    10861 agcgcatctg caaagttata tcgaccgttt ccgtgccaaa gccaccaaag cgaagcaggc
    10921 ccagagccgc attaagatgc tcgagcgtat ggagctaatt gcccccgcgc acgtcgacaa
    10981 cccgttccgc tttagcttcc gcgcgccgga aagcctgcca aatccgttac tgaagatgga
    11041 aaaagtcagc gcgggctatg gcgatcgcat tattctcgac t
//
LOCUS       DAOFQD010000155        11068 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.155, whole genome shotgun sequence.
ACCESSION   DAOFQD010000155 DAOFQD010000000
VERSION     DAOFQD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.155"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            122..1264
                     /locus_tag="QBF46_004145"
     CDS             122..1264
                     /locus_tag="QBF46_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ6676258.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1271..2035)
                     /gene="glcC"
                     /locus_tag="QBF46_004146"
     CDS             complement(1271..2035)
                     /gene="glcC"
                     /locus_tag="QBF46_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDQ6676259.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2286..3785
                     /gene="glcD"
                     /locus_tag="QBF46_004147"
     CDS             2286..3785
                     /gene="glcD"
                     /locus_tag="QBF46_004147"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDQ6676260.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEVLTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3785..4837
                     /gene="glcE"
                     /locus_tag="QBF46_004148"
     CDS             3785..4837
                     /gene="glcE"
                     /locus_tag="QBF46_004148"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDQ6676261.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVTIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLKEAMSEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMIDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4848..6071
                     /gene="glcF"
                     /locus_tag="QBF46_004149"
     CDS             4848..6071
                     /gene="glcF"
                     /locus_tag="QBF46_004149"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDQ6676262.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALDKE"
     gene            6076..6480
                     /locus_tag="QBF46_004150"
     CDS             6076..6480
                     /locus_tag="QBF46_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDQ6676263.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6502..8673
                     /gene="glcB"
                     /locus_tag="QBF46_004151"
     CDS             6502..8673
                     /gene="glcB"
                     /locus_tag="QBF46_004151"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDQ6676264.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWRNFDQIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLKNGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDSAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSSAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9028..10710
                     /gene="glcA"
                     /locus_tag="QBF46_004152"
     CDS             9028..10710
                     /gene="glcA"
                     /locus_tag="QBF46_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDQ6676265.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPMPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2589 a         2765 c         3080 g         2634 t
ORIGIN      
        1 atcattaata aatactattt attcgtcgtt tgtattcatt gggttgatta ttaaggtgcc
       61 gttcgttgct gttgcaacct gtttgtcacc gttaattatt tgttacccac agaacagaac
      121 tatggaatgc gatctgctga tgattaaaat tgaaaaagta attaataaaa atgaccttaa
      181 ggcatttatc gcttttccgt catcacttta tcccgacgat ccaaactgga tacccccttt
      241 attcattgaa cgcaacgagc atttgtctgc gaaaaatccg gggacggacc atatcatctg
      301 gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg cgcaaataga
      361 taccttgcac cgcgagcgtt acggcaaaga taccggtcat ttcggcatga ttgacgccat
      421 tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt tgaagtcaca
      481 aggtgcaagt aagatcagcg ggcctttcag cctgaatatc aatcaggaaa gcggattact
      541 gattgaaggt tttgacacac caccctgtgc gatgatgcca cacggcaaac cgtggtatgc
      601 cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt ggtggatgca
      661 gcgaaccgat ctcactttct ctccggcgct aaaaaaactg atggatcagg tgcgcaaaaa
      721 ggtgaccatt cgctgcatca atcgtcagcg gtttgccgaa gagatgcaga tcctgcgtga
      781 gattttcaac tctggctggc agcacaactg gggatttgtg ccgtttaccg aacatgaatt
      841 cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac gatatgatct atatcgctga
      901 gattgattct gcaccctgcg cgtttattgt cggcttgccg aacatcaacg aggcgattgc
      961 cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc gtttgaaagt
     1021 cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt atcagttcag
     1081 ccgcatcggc ccggtgattg cgctgttatt gattgaagcc ttacgcgatc cgtttgcccg
     1141 ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag gcatgaataa
     1201 catgctggaa cgcattgggg cggagccgta caagcgttac cgattgtatg aaaagcagat
     1261 ttaataccag ctaactcagg ttcatctcca gcggcaccgc cgagcgaatc aaatggtggc
     1321 cttccagctc gatatcgtgg agattctttt tcacggtccg cacatgatcg cgtgctgcgc
     1381 gctgagcgac gtgcggcagc cgctgcaaca ccgcgttgta gatcctcgca tgctggcgat
     1441 cgatctgctt tttttgctgt ggtcgatggt agagattatt tacgctggca aacactgaat
     1501 taaacatcag atcggtcaat gattgcagcg taaacaccag cacctgattg tgagaagcct
     1561 gacaaatggc gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc gaaatctctt
     1621 tgttgttctc actggcggcg agcatttttt cataacagcg ggttatcaca acaaaatcag
     1681 cctgcgttcc cagcgttgcc gccagccttg ccgattcgcc ctccagtaat gcgcgaacgt
     1741 cgagcagatc gtacagcgtt cgcggctgcg tactgaacag atggatcagc gggctggtgt
     1801 cctgcacccg attaagccgt gcgacacgag aatcgcgacc ctgcgccgtt tcaataattc
     1861 cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga gcgtgagaag ccgagctttt
     1921 cacacagtcg acgttccgag ggaagtggct gaccgacctt cagtacgccg tcgataatta
     1981 accgttcgat actctctgca accacttcgc aaatagggcg acgttcatct ttcattcccg
     2041 gacctcgtgc acaggtagga ccaatttttc tgttcttaac tcgcaaaaca cgcacatcac
     2101 gtaagtgatt atgttacatc aatttaacat tgagttaacc aaggcaaggt cacagagctg
     2161 gaaaaaaaat ggtctgaccg gtagctaaag agatagacga aaacgaaaag cccgcttaat
     2221 aactgttcac agaagcagcg cgcaaaaatc agctgccaca caacacaaca aagcgaagcc
     2281 tactcatgag catcttgtac gaagagcgtc ttgatggcgc tttacccgat gtcgaccgca
     2341 catcggtact gatggcactg cgtgagcatg tccctggact tgagatcctg cataccgatg
     2401 aggagatcat tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt ccattactgg
     2461 ttgttctgcc taagcagatg gaacaggtga cggcgattct ggctgtctgc caccgcctgc
     2521 gtgtgccggt ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg ctgccgctgg
     2581 aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat cctcgacatt aaccccgttg
     2641 gtcgccgcgc gcgcgtgcag ccgggcgtgc gtaacctggc gatctcccag gccgttgcgc
     2701 cgcataatct ctattacgcg ccggatcctt cctcacaaat cgcctgctcg attggcggca
     2761 atgtggcgga aaatgccggc ggcgtccact gcctgaaata tggtctgacc gtacataacc
     2821 tgctgaaaat tgaagtgcaa acgctggatg gcgaggtgct gacgctggga tcggatgcgc
     2881 tggattcgcc tggttttgac ctgctggcgt tgttcaccgg atcggaaggt atgctcggcg
     2941 tgaccaccga agtgacggta aaactgctgc cgaagccgcc cgtggcgcgg gttctgttag
     3001 ccagctttga ctcggtagaa aaagccggac ttgcggttgg tgacatcatc gctaatggca
     3061 ttatccccgg cgggctggag atgatggata acctgtcgat ccgcgcggcg gaagatttta
     3121 ttcatgccgg ttatcccgtc gacgccgaag cgattttgtt atgcgaactg gacggcgtgg
     3181 agtctgacgt acaggaagac tgcgagcggg ttaacgacat cttgttgaaa gcgggcgcga
     3241 ctgacgtccg tctggcacag gacgaagcag agcgcgtacg tttctgggcc ggtcgcaaaa
     3301 atgcgttccc ggcggtagga cgtatctccc cggattacta ctgcatggat ggcaccatcc
     3361 cgcgtcgcgc cctgcctggc gtactggaag gcattgcccg tttatcgcag caatatgatt
     3421 tacgtgttgc caacgtcttt catgccggag atggcaacat gcacccgtta atccttttcg
     3481 atgccaacga acccggtgaa tttgcccgcg cggaagagct gggcgggaag atcctcgaac
     3541 tctgcgttga agttggcggc agcatcagtg gcgaacatgg catcgggcga gaaaaaatca
     3601 atcaaatgtg cgcccagttc aacagcgatg aaatcacgac cttccatgcg gtcaaggcgg
     3661 cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta caccgctgtg
     3721 ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc tttccctgaa ctggagcgtt
     3781 tctgatgcta cgcgagtgtg attacagcca ggcgctgctg gagcaggtga atcaggcgat
     3841 tagcgataaa acgccgctgg tgattcaggg cagcaatagc aaagcctttt taggtcgccc
     3901 tgtcaccggg caaacgctgg atgttcgttg tcatcgcggc attgttaatt acgacccgac
     3961 cgagctggtg ataaccgcgc gtgccggaac gccgctggtg acaattgaag cggcgctgga
     4021 aagcgcgggg caaatgctcc cctgtgagcc gccgcattat ggtgaagaag ccacctgggg
     4081 cgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt cggtccgcga
     4141 ttttgtcctc ggcacgcgca tcattaccgg cgctggaaaa catctgcgtt ttggtggcga
     4201 agtgatgaaa aacgttgccg gatacgatct ctcacggtta atggccggaa gctacggttg
     4261 tcttggcgtg ctcactgaaa tctcgatgaa agtgttaccg cgaccacgcg ccaccctgag
     4321 cctgcgtcgg gaaatcagcc tgaaagaagc catgagtgaa atcgcccagt ggcaacttca
     4381 gccgctaccc attagtggct tatgttactt cgacaatgcg ttgtggatcc gccttgaggg
     4441 cggcgaagga tcggtaaaag cagcgcgtga actgctgggg ggcgaagagg ttgccggtca
     4501 gttctggcag caattgcgtg aacaacaact gccgttcttc tcgttaccag gtaccttatg
     4561 gcgcatttcc ttacccagcg atgcgccgat gatagattta cccggcgagc aactgatcga
     4621 ctggggcggg gcgttacgct ggctgaaatc gacagccgat gacaatcaaa tccatcgcat
     4681 cgcccgcaac gctggcggtc atgcgacccg ctttagtgcc ggagatggtg gctttgcccc
     4741 gctatcggct cctttattcc gctatcacca gcagcttaaa cagcagctcg acccttgcgg
     4801 cgtgtttaac cccggtcgca tgtacgcgga actttgagga gcaggctatg caaacccaat
     4861 taactgaaga gatgcggcag aacgcgcgcg cgctggaagc cgacagcatc ctgcgcgcct
     4921 gtgttcactg cggattttgt accgcaacct gcccaaccta tcagcttctg ggcgatgaac
     4981 tggacgggcc gcgcgggcgc atctatctga ttaaacaggt gctggaaggc aacgaagtca
     5041 cgcttaaaac gcaggagcat ctcgatcgct gcctcacttg ccgcaattgt gaaaccacct
     5101 gtccttctgg tgtgcgctat cacaatttgc tcgatatcgg gcgtgatatt gtcgagcaga
     5161 aagtgaaacg cccactgccg gagcgaatgc tgcgcgaagg attgcgccag gtagtgccgc
     5221 gtccggcggt cttccgtgcg ctgacgcagg tagggctggt gctacgaccg tttttaccgg
     5281 agcaggtcag agcaaaactg cctgccgaaa cggtgaaagc taaaccgcgt ccgccgctgc
     5341 gccataagcg tcgggtttta atgctggaag gctgcgccca gcctacgctt tcgcccaaca
     5401 ccaacgcggc aactgcgcga gtgctggatc gtctggggat cagcgtcatg tcagctaacg
     5461 aagcaggttg ttgtggcgcg gtggactatc atcttaatgc gcaggagaaa gggctggcgc
     5521 gggcgcgcaa taatattgat gcctggtggc ccgcgattga agcaggtgcc gaggcaattt
     5581 tgcaaaccgc cagcggctgc ggtgcgtttg tcaaagagta cgggcagatg ctgaaaaacg
     5641 atgcgttata tgccgataaa gcgcgtcagg tcagtgaact ggcggtcgat ttagtcgagc
     5701 ttctgcgcga ggaaccgctg gaaaaactgg caattcgcgg cgataaaaaa ctggccttcc
     5761 actgcccgtg taccctacaa catgcgcaaa agctgaacgg cgaagtggaa aaagtgttgc
     5821 ttcgtcttgg atttacctta acggacgttc ccgacagcca tctgtgctgc ggttcagcgg
     5881 gaacatatgc gttaacgcat cccgatctgg cacgccagct gcgggataac aaaatgaatg
     5941 cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa cattggttgc cagacgcatc
     6001 tggcgagcgc cggtcgtacc tctgtgcgtc actggattga aattgtagaa caagcccttg
     6061 ataaggaata acaaaatgaa aactaaagtc attcttagcc agcaaatggc gagtgcaatt
     6121 attgccgcag gtcaggaaga ggcgcagaaa aataactggt ctgtttccat tgctgttgct
     6181 gatgacggcg gtcatctgct ggcattaagt cgcatggacg attgcgcgcc gattgcggct
     6241 tacatctccc aggagaaagc gcgtactgcc gcgctggggc gtcgtgaaac taagggctat
     6301 gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg cgccgttact gacgtcgctg
     6361 gaaggcggcg taccggttgt tgtggatggg caaattattg gtgccgtggg cgtttctggt
     6421 ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag cagcggtgtt ggcgaaataa
     6481 gcgaaaacga ggagataaac aatgagtcaa accataaccc agggccgttt acgcattgac
     6541 gccaatttta aacgttttgt ggatgaagaa gttttaccgg gcgtagaact agacgctgcc
     6601 gcgttctggc gcaattttga tcagatcgtt cacgatctgg ccccagaaaa tcgtcagttg
     6661 ctggcagaac gcgatcgcat tcaggcggcg cttgatgagt ggcatcgcag taatccgggg
     6721 ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg aactgggcta cctggtgccg
     6781 caaccggagc gcgtgacggt ggaaaccacg ggcattgaca gcgaaatcac cagccaggcg
     6841 gggccgcagc tggtggttcc ggcaatgaac gcccgctacg cgctgaacgc ggcgaacgct
     6901 cgctggggct cactgtacga tgcgttatac ggcagcgaca tcatcccgca ggaaggggcg
     6961 atggtcagcg gctacgatcc gcaacgcggt gagcaggtta tcgcctgggt tcggcgtttc
     7021 ctcgatgaat ctctaccgct gaaaaacggc agctatcagg atgtggtggc gtttaaggtg
     7081 gtcgataaac aattacgcat ccagttgaaa aatggtaaag aaaccacgtt acgtaccccg
     7141 gcacagtttg tcggttaccg tggcgattcc gctgcgccga cctgcatttt gctgaaaaat
     7201 aacggcctgc atattgaact gcaaattgat gccaacgggc ggattggcaa agacgatccg
     7261 gcgcacatca acgatgttat cgtcgaagcg gccatcagta ccattctcga ctgcgaagat
     7321 tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt accgcaatct gctgggcctg
     7381 atgcagggga ctctgcaaga gaaaatggag aaaaacggtc ggcaaatcgt gcgtaaactg
     7441 aatgatgatc gtcagtacac cgccgccgat ggctctgaaa tttctctgca cggacgctcg
     7501 ttgctgttta tccgcaacgt gggtcatttg atgaccattc ctgtgatttg ggacagcgaa
     7561 ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga ctggcgcgat tgccctctat
     7621 gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct atattgtgaa accgaaaatg
     7681 cacggtccgc aggaagtggc gttcgccaac aaactgttta cccgcattga gacaatgctc
     7741 ggtatggcac cgaataccct gaaaatgggc attatggatg aagaacgtcg gacctcgctg
     7801 aacttgcgta gctgtatcgc tcaggcgcgc aaccgcgtgg cgttcatcaa taccggtttc
     7861 ctcgaccgta ccggcgatga aatgcattcg gtgatggaag ctggcccgat gctgcgtaaa
     7921 aaccagatga aatcgacgcc gtggatcaaa gcctacgaac gtaataacgt gctttccggc
     7981 ctgttctgtg ggctgcgcgg taaagcgcaa atcggtaaag gcatgtgggc aatgccggac
     8041 ctgatggcag acatgtacag ccagaagggc gaccaactgc gtgcaggggc aaacacagcc
     8101 tgggttccgt caccaaccgc tgctacgctc catgcgctgc actaccacca aaccaacgta
     8161 cagagcgtgc aagccaacat tgcccagacc gagttcaatg ctgaatttga accgctgctg
     8221 gacgatctgc tgactattcc ggtggctgaa aacgctaact ggtcggtgga agagatccaa
     8281 caagagctgg ataacaacgt gcaggggatt ctggggtacg tggtgcgctg ggtggagcag
     8341 gggattggtt gttcaaaagt gccggatatt cacaatgtgg cgctgatgga agaccgcgca
     8401 acgctgcgca tctccagcca gcatatcgcc aactggttac gtcacggtat tttgactaaa
     8461 gaacaggtgc aggcgtcgct ggagaatatg gcgaaagtgg ttgatcagca aaacgctggc
     8521 gatccggctt atcgtccgat ggcggggaat ttcgctaact cgagtgcttt taaagctgcc
     8581 agcgatttaa tcttcctcgg cgtgaaacag ccaaacggtt ataccgaacc gttattacac
     8641 gcctggcgtt tacgcgaaaa agaaagtcat taatagcgtc aaaaaagccc cggcaagttt
     8701 tattgtcggg gctatgatag taactgcggt agtacattta tcccggaaat aaccggagcg
     8761 attatcaggg tttaacaata ttaaataccc gtagcgcatt actagcgggc ctaaaacaac
     8821 aatatggaca tcttaacgat actctgcgcc tgcgtgtgcg gaacagaaac ccttacccga
     8881 tatacttgtt atgccagagg tagcaattgc gttagcagcg tactgttgct gtaaagtata
     8941 acgcctatat tttacagttc agtcctcttt tgcttattac aaccttaagt atatcaagca
     9001 tataaagata ataagagact gaacaatatg gttacctgga cccaaatgta tatgccgatg
     9061 ggagggctgg ggctatccgc tctggtcgcc ctgatcccga taatattctt cttcgttgca
     9121 ctcgcggtat tacgtctgaa aggacatgtc gctggagcaa taacccttat attatctatc
     9181 ctgattgcaa tattcgcctt taaaatgccg attgatatgg catttgctgc tgcgggctat
     9241 ggctttattt atggattatg gccaattgcg tggattattg tcgcggcggt gttcctgtat
     9301 aaattaaccg ttgccagcgg gcagttcgat attatccgca gctcggttat ctccatcacc
     9361 gacgatcagc gtttgcaggt gttactgatt ggtttctcct ttggtgcgtt gctggaagga
     9421 gcggctggct ttggtgcgcc ggtggcgatt accggtgcgc tgctggtggg cctgggcttc
     9481 aaaccgttat acgcggcggg gctgtgtctg attgccaata ctgcgccggt ggcgtttggt
     9541 gcgttgggcg tgccgattct ggtcgccggt caggtaacgg gaatcgatcc gttccacatt
     9601 ggcgcaatgg cgggacgtca gttaccgttc ctgtcggttc ttgtgccgtt ctggctggta
     9661 gcaatgatgg acggctggaa aggggtgaaa gagacgtggc cagcggcgct ggttgctggg
     9721 ggaagcttcg ctgtcactca gttctttacc tctaactata ttggtccgga actgccggat
     9781 attacttcgg cgctggtgag tatcgtctca ctcgctttat tccttaaagt ctggcggccg
     9841 aaaaataccg aaacggcaat cagcatggga caatccgcag gtgcgatggt ggtaaataag
     9901 ccatcttctg gcggtcccgt gccttcagaa tatagtctgg ggcaaatcat tcgtgcgtgg
     9961 tcaccgtttt taatcttaac ggtgctggtc accatctgga ccatgaagcc gtttaaagcg
    10021 ttatttgctc cgggcggcgc gttttattca ctggtgatta atttccagat ccctcatttg
    10081 catcaacaag tgttgaaagc ggcacccatt gtcgcccaac caatgccaat ggatgcggtg
    10141 tttaaattcg accctctctc ggctggcggc accgctattt ttattgcggc gattatctct
    10201 atcttcatcc tcggcgtggg catcaagaaa ggtattggcg tctttgccga aacgctaatt
    10261 agtctgaagt ggccgatatt gtcgattggc atggtgctgg cgttcgcctt cgtcaccaac
    10321 tattccggca tgtccaccac gctggcgctg gtactggcag gtacaggcgt gatgttcccg
    10381 ttcttctcac cgtttctcgg ctggctgggc gtattcctta ccggctcgga cacctcctct
    10441 aacgccctgt ttggttcact gcaatcgacc acggcgcagc aaatcaacgt ctctgacact
    10501 ctgctggtgg cagcaaatac cagcggcggc gtaactggca agatgatctc cccgcaatct
    10561 atcgccgtgg cttgcgccgc gacgggcatg gtgggccgag aatctgaact gttccgctac
    10621 accgtgaagc acagtctgat ttttgccagc gttatcggca ttatcaccct gctgcaggcg
    10681 tatgtgttta ccgggatgtt agtctcgtaa atcaataaag ccggatgatc gttaatatca
    10741 tccggcttca tttacattat gctgtcttat taattgaaaa ttatttctcg ctttattatc
    10801 catgcagcaa taatgttttg cgttcagata acctgctaaa taattaagag cgtgagaaca
    10861 tctatttttt tatcacgcaa aacaagtctg tattaattat gttcgcttga tgagcaaaca
    10921 ccgttctatc tgttgctttt gcaatcttaa tcgttaaaac taccaacaaa tttatatagt
    10981 tacgttgtga aatgttgtta attttttgtt tattgaaatt tcgtaaaaat aaccagccag
    11041 acatcaaata ggggttgttc ttataaat
//
LOCUS       DAOFQD010000156        11045 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.156, whole genome shotgun sequence.
ACCESSION   DAOFQD010000156 DAOFQD010000000
VERSION     DAOFQD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.156"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(293..1588)
                     /locus_tag="QBF46_004153"
     CDS             complement(293..1588)
                     /locus_tag="QBF46_004153"
                     /inference="COORDINATES: protein motif:HMM:NF017116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676266.1"
                     /translation="MSNHEENSGAVADNVTPITAAKVGRPSLPKKYPTTAFPVELEIN
                     YRKGRYSSSLENVVAAMRCHSVTGIQLALDEFFAEEGAFVNGQWYPLSDNLVTELRLR
                     LAAADFEKVTKDNARDAMFYVCGENKFDSAQRWLAAQEWDGKPRVDMFFERCFKCQGD
                     PEYLAAVSLYTWTALAGRVMNPGIKADMVPLLYGRQGARKSTGVRLMSPFPDSYSSFN
                     LNDKDDSLVRRMRGCLIGELEELRGLHGRDSESVKAFFSAAHDEWVPKFKEFKVRNPR
                     RVVFIGTTNNKQILNDLTGNRRYLPMTSGDVDCAAIEKNRSQLWAEALHLFNENGVMW
                     EAAETLARKVHDEFRVEDPWEDEIADLLKIGVDDCEPTSFVPTKALFAHLGMYLSHTS
                     NAQNQRLNAIMTRLGYEKGQRRIKGATVRGYFRTGSSTL"
     gene            complement(1824..2114)
                     /locus_tag="QBF46_004154"
     CDS             complement(1824..2114)
                     /locus_tag="QBF46_004154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676267.1"
                     /translation="MGWPLRVVRFVVLFVLRSVMRFVVRVEFDVVQLSCAEGRRRGVA
                     LGACGQFLWTSILRRGWPVDKSRRLRGSKIGAGSRLSLRDRYVLAQGVGVTV"
     gene            2113..2601
                     /locus_tag="QBF46_004155"
     CDS             2113..2601
                     /locus_tag="QBF46_004155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676268.1"
                     /translation="MREKTRTLTVRLPVSLIERIEQRAEKTKVTRTDAVIEALVAGLG
                     TKPEGMDTKLLTAIEALRSEVRALAGGGKDARQSAPQTARQSAPQNARQIVPRGADPR
                     FQAVSDEADQLIVSMTGENATLPAIAQALNAAGHTTATGQEWNTSRVRDYRHKLRERG
                     LI"
     gene            complement(2619..3077)
                     /locus_tag="QBF46_004156"
     CDS             complement(2619..3077)
                     /locus_tag="QBF46_004156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676269.1"
                     /translation="MKSKTELRAAATARALEVIASEMGGWSLDGFAHGSLPNFSPLPR
                     QQTQEGSVVLERPPFDYTWAGTAAFTDRANRALQVKLPASRERNYIWLCAVEREAVAT
                     ALIVESFNVTGCAAFAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAAA"
     gene            complement(3308..4480)
                     /locus_tag="QBF46_004157"
     CDS             complement(3308..4480)
                     /locus_tag="QBF46_004157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676270.1"
                     /translation="MAFDNSHLEDAGLFWRAVSSAPAPVDRDEWLKMARGLTVWASLC
                     GEDGRAALAAWSERIGRGDRHHASQFGHLVRIGTDGLKNPEAMAQTFLQFSGVEWNRQ
                     ASVQSDADRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKT
                     ALAAAGGVQLPALSTDLGVGGMFRSFNPRAYWSDCLLIPVYKFDAARQLYVDGLEAIC
                     GRCSAEGFQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFA
                     LHHLSRGKVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDER
                     EPVPTIPGAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
     gene            complement(4467..4676)
                     /locus_tag="QBF46_004158"
     CDS             complement(4467..4676)
                     /locus_tag="QBF46_004158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676271.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYW
                     LAGNPEIKRFVADQRRPGRAADGVR"
     gene            5032..6321
                     /locus_tag="QBF46_004159"
     CDS             5032..6321
                     /locus_tag="QBF46_004159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676272.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCS
                     LRVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCP
                     RCSRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGW
                     QVWYGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHREPLRWNGGDG
                     HAGEVELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSD
                     CKAAPLVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFTQNETRS
                     LAKIPVILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLTLSVLAAVGR
                     WDYINDIIRRIDRDRGDAATLIALADHIQTNRPERDL"
     gene            6318..6959
                     /locus_tag="QBF46_004160"
     CDS             6318..6959
                     /locus_tag="QBF46_004160"
                     /inference="COORDINATES: protein motif:HMM:NF016255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676273.1"
                     /translation="MTEPTRPILTIKRRNRTVYVPPEKATKSEPQPAERTTSKAKVQP
                     KAKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKA
                     FLKEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSY
                     QQSLIDQCMRYDLDGNPTVVVTPEEAEQAKARLTEIKAFRRKARQAKGEKKGA"
     gene            complement(6979..7164)
                     /locus_tag="QBF46_004161"
     CDS             complement(6979..7164)
                     /locus_tag="QBF46_004161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676274.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRA
                     APTWRWVRREQPRSTGR"
     gene            complement(7157..7519)
                     /locus_tag="QBF46_004162"
     CDS             complement(7157..7519)
                     /locus_tag="QBF46_004162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676275.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSV
                     TERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFQRKRTRELVASDPDVAAAGEAL
                     EQRANGVNLSETFGGGDE"
     gene            complement(7512..8084)
                     /locus_tag="QBF46_004163"
     CDS             complement(7512..8084)
                     /locus_tag="QBF46_004163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676276.1"
                     /translation="MSAFKLLCDRLRNEATLAKSDGAGAFKKQSLDPDLKGGKKGKKP
                     AGELEEDEAIASLHRKRMNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTF
                     APIGAPVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAA
                     RAPAFAEVRRQVQAGGMTLSPAARGIDWNA"
     gene            complement(8084..8743)
                     /locus_tag="QBF46_004164"
     CDS             complement(8084..8743)
                     /locus_tag="QBF46_004164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676277.1"
                     /translation="MTEIKTECPDAAWLRTTLAEMAGDAFPVYDLPSLRVDPNSSTQL
                     SALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVS
                     PDAGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRV
                     QRIELLLKLAMDEATTLAEADGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEG
                     V"
     gene            9003..9590
                     /locus_tag="QBF46_004165"
     CDS             9003..9590
                     /locus_tag="QBF46_004165"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012464.1,HMM:NF014814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6676278.1"
                     /translation="MNKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVP
                     PRERPALGILLNQLLQEGDTLVIYAIDRIGRNARDTLELVEELEKRKITLVSIREGVD
                     LSSAVGQAVLGVMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVM
                     RRAMGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
     gene            complement(9671..9925)
                     /locus_tag="QBF46_004166"
     CDS             complement(9671..9925)
                     /locus_tag="QBF46_004166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676279.1"
                     /translation="MLLTPVRLSLWSCLPCVKADDADGAVSPCLVAEGSLVLASAVGG
                     AMTRLAKLADLAVFDAQARVAGRGGLLYALHVIKPLTGGW"
     gene            10143..10610
                     /locus_tag="QBF46_004167"
     CDS             10143..10610
                     /locus_tag="QBF46_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6676280.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRRWASEK
                     AAAPSNVLSIEEMRKRAAAAVEATPQAAIRRLKNHFVQQQFDLLQRHAKDLQALRSAS
                     LQAMLEKDATMVKAISGLMTALLKAQERERLIYEIKPGTEADIMREGMNRKQQ"
BASE COUNT         2512 a         3691 c         3149 g         1693 t
ORIGIN      
        1 aaaaaaaata tttaattgtg taattagtga gcgatttacc cctatttacc ctattaattc
       61 gttttttata tttaattaga aaagtgggtg tgacgggtgt gacacttaat taaatactaa
      121 gtaaataaag ggattagttg tcacaggcac gggtgtgacg tgggtgtgac gggtgtgaca
      181 ggtgggtgtg acgggtggga caaaaaatgc aggtgtcaca cccgggtgtg acggtgggtg
      241 tgacacataa aaaaggggag cattaagctc ccctggaagg tgaaagtgac cgctacaggg
      301 tcgatgaacc agtgcggaag tacccgcgaa cggttgcacc tttgattctt cgctgccctt
      361 tctcgtagcc gagacgcgtc attatcgcgt tcaggcgctg gttttgagca ttgctggtat
      421 ggctcaggta catcccaagg tgagcgaaca gcgccttagt cggaacgaag ctggtcggct
      481 cgcagtcgtc gaccccgatc ttgagcaggt cggcgatctc gtcctcccat ggatcctcaa
      541 cgcggaactc gtcatgtacc ttacgcgcca gagtttcagc ggcctcccac atcaccccgt
      601 tctcgttgaa caggtggagc gcctcagccc acagctggga gcggttcttc tcgatagccg
      661 cgcagtccac gtccccggag gtcataggca aatagcggcg gttacccgtc aaatcattga
      721 ggatctgctt gttgtttgtg gtaccgatga acacgacacg gcgggggttt cgtaccttga
      781 actctttaaa cttcggcacc cactcgtcgt gggctgcgga gaagaacgcc ttcaccgatt
      841 cggagtcgcg cccatggagg ccgcgcagct cctccagctc gccaatcagg cacccacgca
      901 tacggcgcac taaggagtcg tccttgtcgt tcaggttgaa gctggagtag gagtcaggga
      961 acggcgacat gaggcgaacg cccgtcgatt tgcgggcacc ctgccgcccg tacagcagcg
     1021 gaaccatgtc ggccttgatg ccgggattca tcacgcgccc tgcaagcgcc gtccaggtgt
     1081 agaggctgac cgccgccagg tactcgggat ccccctgaca tttgaagcac cgctcgaaga
     1141 acatatcgac gcgaggcttc ccgtcccact cctgagccgc cagccagcgc tgggcgctgt
     1201 cgaatttgtt ctcaccgcag acgtagaaca tcgcgtcgcg ggcattgtcc ttggtcacct
     1261 tctcgaagtc ggcagcagcg aggcgcagac gcagctcggt caccaggttg tcggacagcg
     1321 ggtaccattg cccgttcacg aacgccccct cttcagcgaa gaactcgtcg agcgcgagct
     1381 ggatgccggt gacggagtgg cagcgcatag cggcgacaac gttctcaagc gaggatgagt
     1441 agcgcccctt cctgtagttt atctccagct ccacgggaaa cgcggtggtc gggtattttt
     1501 taggcagcga ggggcgtccc accttagcgg cggtgatcgg cgttacgttg tcggcaacag
     1561 cgccggaatt ttcttcatgg tttgacaatt ttacgtcctc aaaattgaac agccaatctc
     1621 aaggagcctt acaacggcgg gactttacga ggaaaacacc ctttgcacgc gtcattatga
     1681 tcgcgtataa ttcacgctgg cttcacaccc aacatgagtt attatgtgtt ttccacggtg
     1741 accgccgtga aagctccaaa ctcaaaacag ctttgaagaa aacccgtctc cccaaggcgg
     1801 gtttttcttt ttcagccgcg ccatcatacc gttacaccca ccccctgcgc cagtacatag
     1861 cgatcccgca gcgataaacg cgatcccgcg ccgatcttag agccgcgcag cctccgcgac
     1921 ttatccacgg gccagccccg ccgcaaaata cttgtccaca gaaactgtcc acaggcaccc
     1981 aaggcgacgc cacgccgtcg cccctcagcg cacgacaatt gcactacgtc aaattcgacg
     2041 cgcacgacaa aacgcattac actccgcaag acaaacagca cgacaaaacg cacgacacgg
     2101 agaggccagc ccatgcgaga gaaaaccaga accctaaccg ttcgactgcc cgtcagcctg
     2161 atcgagcgca tcgagcagcg agcggagaaa accaaagtaa cccgcacaga tgccgtcata
     2221 gaggcgcttg tcgccggact cggcacgaag ccggaaggga tggacaccaa acttttgacc
     2281 gccatcgagg cgctgcggtc agaggtcagg gcgctggccg gaggcgggaa agatgcacga
     2341 cagtctgcac cacaaacagc acgacaaagc gcaccacaaa acgcacgaca gatagtcccc
     2401 cggggagccg atccccgctt ccaggccgtc agcgatgagg ccgaccagct catcgtcagc
     2461 atgaccgggg agaacgccac cctgcccgcg atcgcccagg cgctgaacgc agccgggcac
     2521 accaccgcca ccgggcagga gtggaatacc agccgcgtcc gggactaccg ccacaagctg
     2581 cgcgagcgcg gcctgatttg acgcagaggg gcgacggctc acgccgccgc cgcatccagc
     2641 cacggcagca tcgccgccct gatcagctcc acgtcggcct cgtccatggt cagcagcacc
     2701 atgccatcca caggaggcaa cccggcgaac gccgcgcagc cggtcacgtt gaacgactcc
     2761 actatcagcg cggtggccac agcctccctt tcgaccgcgc acagccagat gtagttacgc
     2821 tcacgcgacg ccggtagctt cacctgcagc gcacggtttg cacgatcggt aaacgcagcg
     2881 gtacccgccc aggtgtagtc gaacggcggg cgttcgagca ccacagagcc ctcctgcgtc
     2941 tgctggcgcg gcagcgggga gaagttcggc agcgagccgt gagcaaaccc gtcgagcgac
     3001 caacccccca tctcagaagc gatcacctca agggcgcgag ccgtcgcggc agcgcgtaat
     3061 tcagtctttg atttcatgtg ccctccttac gggcgaaaaa aagcccgaca ccaaaacggt
     3121 gccgggcttg agtgtgctgc tggaaaccaa accagctgca ggatagcgtc acgaccgcag
     3181 cggcggtgcg tcgctttaat ccgtctatgc aaaaaagcgt cgatagtgtc tcgaaagtcg
     3241 gtcgcaaaat ccggggcagc ggcagcagga ggtgtgtccc gaaaggcgca ccattcggac
     3301 acgtcaatca acgccgctca agccgggcat gacgaaccgc ataccggagc agctcacgtg
     3361 cccgatcttc cccttcctca accagcacgt cgaaccagtc gaggttatcc cggggagtga
     3421 accgatcgag gccgtcgaac gacacaaccg ccgcgcctgg aattgtcggg accggctctc
     3481 gctcatcgcc ataggccgct ttatgcgcca ggcggtacgc ttcgccgtga tccggtacca
     3541 gcatcacatc aacatctggc cagaaccgac gcagcgacgc cacgcagctg ccgaacccgg
     3601 cgatcgagaa cgcagcgcag accgccacct tgccacggct caggtggtgc agggcgaacg
     3661 ccgtagcgaa cccctcacag acgaacagag gcgagtaggg cgcgtgcggg gagccgtcga
     3721 gcacgtccat gaaccccggc atcgggtaga agccgccctg cttgcgggta cccgccggag
     3781 agaacttcga gccagtgagc tggaaaccct cagccgagca acgcccgcag atggcctcca
     3841 ggccgtcaac atacagctga cgggcggcgt cgaacttgta aaccggaatc aggaggcaat
     3901 cagaccagta agcacgcgga ttgaacgatc gaaacatccc gccaacacca agatctgtgc
     3961 tgagggcggg cagctggacg ccacccgcag ccgccagagc ggtctttttg cgccagtatg
     4021 gggtagacgc aggatcccct cccttcgcca gcagcttgac cgcagcacgc gcagcagcat
     4081 cacccttctt gtcgcgttca gcctttgcct tcgctgtggc ctcacgggac aaccgcagcc
     4141 gttcagcgcg atcggcgtcg gactgaacag aagcctgtcg gttccactcc acgccggaga
     4201 actgcaggaa ggtctgcgcc atcgcctcgg gatttttcag accgtcggtg ccgatacgca
     4261 ccaggtgacc gaactggctc gcgtggtgcc ggtcacctcg cccgatccgt tcagaccagg
     4321 ccgcaagagc ggcgcgtcca tcctcgccgc agagagacgc ccacacggtc agaccgcgag
     4381 ccatcttcaa ccactcatcg cgatccaccg gagccggggc agaggagaca gcacgccaga
     4441 acaggccagc atcctcgagg tgagagttat cgaacgccat cagcagccct ccccggacga
     4501 cgctggtcgg caacgaagcg tttgatctcg ggattgccag ccagccagta atgcccgcca
     4561 cggagggagc gccctgccgg agtcggcacg atcccccgcg aacttagccg cgagagcgtg
     4621 cgcagcgaca cgttcagcag cgcagcgagg tcagcggtat agattcgttt tttcattggg
     4681 agtaccagat aggtgccccc ctcagcttaa cgggcatcgg ggggcgggag tgacgattat
     4741 cgataaagcc gcccttgagg acttaccgca tagcggcagg acagcagccc ttctcgctta
     4801 ctcgcacctg ccaaggacga agcgcgcatc ctcgtttaca accccgcctc gttggggagg
     4861 agggaaagcg gcaccgggcg atccggtttc atgtttccca actgctcgtc aggaatcagt
     4921 aagacaagcg taacacaatt aacgccaaag tggcgttttt tgtgagatac taaaaaagac
     4981 gcacagccag tgctggcgct gtctggcgac catttcgacg cagggggagc agtgaacgac
     5041 gataacgaca cgaacgaatc gacgcagccg aacccggcag cacttgaacg cgtaagcgcg
     5101 actgaaaaat tccccgagca gctccccggc gttttgttcg tcacctgccc cggcggatgc
     5161 tccctccggg tcgaggcatg ccgacagatt tatgagcaga gccacctcga tcgcacgatt
     5221 gaaaaccccc acctcgagtg ccaccggtgc cccactggcc tcgcgctttg ggtcggtacc
     5281 gaacagcccc ctgacgcacc actgaccacc ccggcagaca cccgtaagct ctgcccccgt
     5341 tgcagccgga aagacaaccg cctgatcgcc cccaataagc ccggttcgag cctgtgcgtc
     5401 tcctgctgga accgcgaacg cgaagccacc atcggagcca acgcacgcgg gagagtcaac
     5461 cgcaccccac cgttcgtctc gccgttcctg atcggcatcg gcgatggctg gcaggtctgg
     5521 tacggcatcc accagggcga ggcgctcgtc cgcgccatgc gagccaaccc gggcgcacgg
     5581 ttccacgatc gccgaccagg cccggtcggg caaacacgcc gcgacggttt cgtgtactac
     5641 tgccacgaac accgcgagcc cctgcgctgg aacggtggcg acgggcacgc cggagaggtg
     5701 gagcttgctt gtcccaggtg ccagccatgg gcggcacccc tcagcgtcgc ccccacgttt
     5761 cagacggtca gcttccagac accgcatgac gccgcgcacc agtaccggaa cgccaccctg
     5821 ccagacaacg cggtctggac agcgtcggtc tgctccgatt gcaaagccgc gcccctggtg
     5881 atcagcaagg caccggtcaa gggcaagccg cgacgggaga tcgtcgtctc ctgcccgtcc
     5941 tgcgactcca gcgcatcagc caggcccgga ggcaggacat ggacagacga gcacatcgcg
     6001 gtgcccgagt tcacgcagaa cgagacgcgc agcctggcga agatccccgt tattttgccg
     6061 cagagggagc agccgcagct gctcaaaaga ccagggacag ggcgcggtcc aggacgcccc
     6121 cgcaagcgca tccccctgac cctcgaccgg gtaccagaga gtcggttcaa ccgcctcacc
     6181 ctttccgtcc ttgccgccgt cgggcgctgg gactacatca acgacatcat ccggcgcatc
     6241 gatcgggacc gcggcgacgc cgccaccctg atcgcccttg ccgaccacat ccagaccaac
     6301 agacctgaga gagatttatg accgaaccca cccgcccgat cctgaccatc aagcgacgca
     6361 accgcacggt ctacgtcccg ccagaaaagg ccacgaaatc tgagccacag ccagccgaac
     6421 ggactaccag caaggcgaag gtacagccaa aagcgaaagc cccgcagaag cccgcacaga
     6481 agcccacagc ggcaaagccg aaaaagacgc ccgagcaggt cgccgagggg atccgcagac
     6541 aggaggagaa cgaccgccgc cagcaggaca tcgcgatcgc caacaagtcc aaaaaggcgc
     6601 tggccaaccg ggtaaccaaa gcattcctga aagagcactg gcctgactta ctgacccggg
     6661 gagttgaccg tcgccccatg gcaaagggca tcatcgagca gatcctgacc cgaggcgcag
     6721 agctggacat gccgccagag gcagaccgca aagccgtccg tcgcatcctg ggcattgtca
     6781 cgggtaccag ctcataccag cagagcctca tcgaccagtg catgcgctac gacctggacg
     6841 gcaacccaac cgtcgtcgtc acgccagagg aggccgaaca ggccaaagcc cgcctgacag
     6901 agatcaaggc attccggcgc aaggcacgcc aggcgaaggg cgaaaaaaag ggcgcataac
     6961 gcgccccgtc tcaccgtttc accgccctgt cgaccggggc tgttcccgtc tcacccagcg
     7021 ccacgtaggc gcagcacgac gctctgcagc acggcgagca gctgcgacac gttgagcctg
     7081 tccccttgcc cgtcgggcag caatgacccc gtcggggatc accacctcag gaatgaccag
     7141 gcgcgagaca gcaaattcac tcatcgccac ccccgaacgt ctcggacagg ttcacgccgt
     7201 ttgcacgctg ctccagcgcc tcgccagccg cagcaacatc gggatccgaa gcaaccagct
     7261 cccgggtgcg cttgcgctgg aaccgcagtc ccagcgcgta cagagacgca tccgaacgga
     7321 cgaaagcatc cgtctggcca cccgcagcga gcgatccccg gaacctgcca agcgcccgcc
     7381 gctcagtgac cgatcgatcg cccgggtgga gccgcagaac gggcaacagc cagatcagct
     7441 ccagccgttg cgagcgcgtg atcctgctgg acagcggcat caacacctcg ccctgcaggt
     7501 gacgcggcag cttacgcatt ccagtcaata ccacgcgcag ccggtgacag cgtcatgccg
     7561 cccgcctgaa cctgacggcg cacctcggca aacgccggag cacgcgccgc gtctggcatc
     7621 ccacgagagg acgtcaacac gccgtcaagt aacaccaggt cagccggtgc cacacccgcc
     7681 gctttcatct cgttcaggaa cgccgcgtcc tggatggact gcgcacgcgc acggtgaacc
     7741 gcttcggtcg gagacgccac cggagccccg atcggcgcga acgtctgcgc tgcgccgcct
     7801 gtctccacca ctgtctcgac caccgacgac agcggaggga ggccaaagtc atcagcgccg
     7861 gtggatttgg tcagcgtctt gcaaccttta acgtcgttca tgcgcttacg gtggagagac
     7921 gcgatcgcct cgtcctcctc cagctccccg gcgggctttt tgcccttctt gccgcccttc
     7981 aggtcgggat ccagagattg ctttttgaaa gcgccggccc cgtcagattt tgccagcgtc
     8041 gcctcattgc gaagccggtc acacaggagt ttgaaagcag acattacaca ccctccatgc
     8101 cacggttacg gcgcaggatt tcaatagcgc cctgcgtggc gaaaatttgg ttaagacggc
     8161 ggtcaggcgc gatcacggta cggtacaggc cgcgcccgcc gtcggcttca gcgagcgtcg
     8221 tcgcctcgtc catcgccagc ttgagaagca gctcgatacg ctgcacgcga acggagtgcg
     8281 cccagttgaa cagcgccagc gcagccgccg cgtcacggcg atcggcatcc tgcagcaggg
     8341 tgtcacgctc ccgggccacg tcggcctcag cagcctcagc aacaaccagc tcggcctcga
     8401 tgtccggcag caggaacgac gcaacacccg cgtcggggct caccggctcg ccagcacgca
     8461 gcgcagcctt gccgtcggcg atcaagcccc gcagccgttc ggcctcggtc ttgagccctt
     8521 ccaccactcg agcggcgtcc agccgtcgcg cctctacgtc ggaagcggcc tgacgcattt
     8581 cacgcgcagc ctgacgatca gcgagcgccg aaagctgagt ggaggagttc gggtctaccc
     8641 gcagggacgg gaggtcatac acagggaacg catcacccgc catttcagca agcgtggtac
     8701 gcagccaggc cgcgtccggg cattcggttt tgatttcggt catggtttgg ttctcagcaa
     8761 gttatcagca aagcggcaag aaacgccgct cagacagcat gccgtcacga cgcagcgcag
     8821 acatcaacgc agagggcgca gggctctcgc ccagcgcggg gagccgcctg gaggccgtcg
     8881 ctaatcccct acatttcagg acacccaaca gaacgacgaa aaagacactt tagcgataga
     8941 ctttcgatac agttgtggca tgttgtgtcg aaagagacgt atcggtcaga ggagagaaaa
     9001 aagtgaacaa aacatacggt tacgttcgcg tatcaaccga cgaacaaacc agcaacagcc
     9061 agcgagacgc catcatcgcc cggttcccaa ccgcctccat ccttgccgac gacgggatca
     9121 gcgggacggt accaccacgc gagcgccctg ccctcggcat cctcttgaac cagttgctgc
     9181 aggagggcga caccctggtc atttacgcga tcgatcgcat cggcaggaac gccagggaca
     9241 ccctcgagct ggtcgaggag ctggagaagc ggaagatcac cctggtcagc atccgcgagg
     9301 gcgtcgacct gagcagcgcc gtcggacagg cggtactcgg cgtaatggcc agcttcgccc
     9361 agatggaacg caagctcatc ctcgaacgcc aggcagcagg tatcgaggca gcaaagagcc
     9421 gaggccagaa gttcggacgc ccgaccaaga tcggagcgaa ggaagccggg gagattgtca
     9481 tgcgccgcgc catgggagtg ccgatcgcca ccatagccaa acagctaaac ctaagccgga
     9541 gcgcgattta ccgccatctt gagaaggtcg acgcggtcgc gccggagtaa gagagagatc
     9601 gcggcggtgg tactcgctaa agtcaccgcc gcaacccagg cccggggaag tgtgagagcc
     9661 aacccgggca tcaccaacca cccgttagag gtttaatgac atgcagagca tacaacagac
     9721 cacccctgcc cgctacccgg gcctgagcat cgaacacggc gagatccgcc agtttggcaa
     9781 gccgcgtcat tgcgccgcca accgccgagg ccagtaccag actaccctcc gcgaccaggc
     9841 agggcgatac cgcaccgtca gcatcatcag cctttacgca tgggaggcat gaccataacg
     9901 aaagccttac aggtgtaagc agcaagcaat aacaaaagcc ttacgccatt acgaaccact
     9961 tacaggggcg cttccgcgcc cccttcacca gaacgcacca gagccattct aagcgcctca
    10021 gccacaaaaa cacccaaccg cacgaccacg cacgcacaaa cgccgtgtac cccccaaaaa
    10081 cccccgctat tttcagcgcg tgacgtttca atacgggaga gaaaccaacc ggagcaggaa
    10141 ccatgaagaa atacaccacc gaacagaaag cccaggcgct caggctactg gagcaggacg
    10201 gcgcaaccag tgccaccgtc gcacgcacca tggggatccc accccgcacc gttcgccgat
    10261 gggcgagcga gaaggccgca gcacccagca acgtcctcag catcgaggag atgcggaaac
    10321 gcgccgccgc cgccgtcgag gccacaccac aggccgcgat ccgacgcctt aaaaaccact
    10381 ttgtacagca gcaatttgac ctgctacagc gccacgcaaa ggacttgcaa gccctccgca
    10441 gcgcatcgtt acaggccatg ctggagaagg acgccaccat ggtcaaagcg atcagcggcc
    10501 tcatgaccgc cctgctcaaa gcgcaggaac gcgaacgcct catctacgag atcaagccgg
    10561 gcaccgaggc cgacatcatg cgcgagggca tgaaccgcaa acaacaatga ccgccatgac
    10621 cgaaatgacc gccatgtgac cgtttttgtg accgccaaaa cccgggagcc cacggaatac
    10681 gcggatccgc acgttacgca attgttaccg ctcgcgtgcg cgagaagccc cgttttgatg
    10741 cccggacgaa ccaaacgcgc agctgcagca acgcattcca cccataacgc agagggcgcg
    10801 agggcaagcc ctcacgcaga accaacagac gaccacccag cgcgagcgac agctcgtgcc
    10861 ctcagcgcca ccccaaccac ccgatccacc ccctaaccac taccacccac caccccggca
    10921 cgacgaacct atccaccgat cgaccaagac aagcaaagtt catccccgaa ggggtgcagc
    10981 tgcggcgcgt caatggctcg atgtcacacc cgtcacaggc gtaacggcaa agattcataa
    11041 atagt
//
LOCUS       DAOFQD010000157        10988 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.157, whole genome shotgun sequence.
ACCESSION   DAOFQD010000157 DAOFQD010000000
VERSION     DAOFQD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.157"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            127..888
                     /gene="adiY"
                     /locus_tag="QBF46_004168"
     CDS             127..888
                     /gene="adiY"
                     /locus_tag="QBF46_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDQ6676281.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1025..2362
                     /gene="adiC"
                     /locus_tag="QBF46_004169"
     CDS             1025..2362
                     /gene="adiC"
                     /locus_tag="QBF46_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDQ6676282.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2466..4109
                     /gene="eptA"
                     /locus_tag="QBF46_004170"
     CDS             2466..4109
                     /gene="eptA"
                     /locus_tag="QBF46_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDQ6676283.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLRKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4106..4774
                     /gene="basR"
                     /locus_tag="QBF46_004171"
     CDS             4106..4774
                     /gene="basR"
                     /locus_tag="QBF46_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDQ6676284.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4775..5875
                     /gene="pmrB"
                     /locus_tag="QBF46_004172"
     CDS             4775..5875
                     /gene="pmrB"
                     /locus_tag="QBF46_004172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDQ6676285.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKMQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5851..5940)
                     /gene="pmrR"
                     /locus_tag="QBF46_004173"
     CDS             complement(5851..5940)
                     /gene="pmrR"
                     /locus_tag="QBF46_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDQ6676286.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6052..7554)
                     /gene="proP"
                     /locus_tag="QBF46_004174"
     CDS             complement(6052..7554)
                     /gene="proP"
                     /locus_tag="QBF46_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDQ6676287.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7818..8696)
                     /locus_tag="QBF46_004175"
     CDS             complement(7818..8696)
                     /locus_tag="QBF46_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDQ6676288.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLLEKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGEHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8693..10921)
                     /gene="crfC"
                     /locus_tag="QBF46_004176"
     CDS             complement(8693..10921)
                     /gene="crfC"
                     /locus_tag="QBF46_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDQ6676289.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIKKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALGVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQGTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2759 a         2863 c         2691 g         2675 t
ORIGIN      
        1 tgtaatatta gtaaaactcg ttatttttat gcatgtttat attcatcata caattatata
       61 accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc actcaggagt
      121 acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga aaaagatgtc
      181 tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat ggcgttatta
      241 aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt tgctcatatt
      301 agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc gcaaatacca
      361 gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc agacgtcttt
      421 cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa ggaacgaaca
      481 cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt cctttcatta
      541 atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat tgaaagcgat
      601 attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag cccaagtctg
      661 ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac cacctgccgc
      721 atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca ggtatcacag
      781 tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt ctacggtatg
      841 acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt tttaacctta
      901 acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa ttcaactatc
      961 gaatgcaggt gtatgatatg acgtaattta ttgtcacgaa gctcgccttc gcaggagttt
     1021 aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca ccctgatggt
     1081 gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg cctctactgg
     1141 cgggattgcc atttatggat ggttggtgac gattatcggt gcactggggc tctcgatggt
     1201 atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt acgcccgccg
     1261 ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg cctgctggat
     1321 cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct tcccgattct
     1381 gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct tcgtcctgct
     1441 gaacattgtc ggtccgaaaa tgatcacccg tgtgcaggca gttgccaccg tgctggcgct
     1501 gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg aaacctatat
     1561 ggcggcatgg aacgtcagcg gcctgggcac cttcggtgca attcaaagta cccttaacgt
     1621 tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg tggtgaaaaa
     1681 cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg ccgtttgcta
     1741 tgtactttct accaccgcga ttatggggat gatccctaat gccgcactgc gcgtttctgc
     1801 ttcgccattc ggtgatgccg cacggatggc gttgggtgac accgccgggg ccattgtttc
     1861 cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc tggcgggtca
     1921 aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc gtgtaaataa
     1981 agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca tcttccagct
     2041 cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg tctcggtcat
     2101 ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg gacacggtca
     2161 ctttggtaaa gcacgcccgg catatctggc agttactacc attgccttcc tctactgcat
     2221 ctgggccgtg gtggggtccg gagcgaaaga ggttatgtgg tcatttgtca ccctgatggt
     2281 catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt atcccttaga
     2341 tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc caaccttaag
     2401 aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc gatggaaaca
     2461 ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc tggctattgt
     2521 tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag gtgttgcagg
     2581 cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg gtcgtcgctt
     2641 tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat cgaccactgg
     2701 cctgcctgtt tattctggtt ggcgcggctg cacaatattt cataatgact tacggcatcg
     2761 tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccggcagaa agttatgcgc
     2821 tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct gcgctgattg
     2881 cctgctggat aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt ttccgtggag
     2941 ccaatattct ggtttctgta ctactgattt tgctggtcgc cgcactgttt tataaagact
     3001 acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc tctaacagca
     3061 ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg ctggtgcgaa
     3121 ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa aatttgacca
     3181 tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc tacccgcgtg
     3241 aaactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc gcatcttgcg
     3301 gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt gagcattaca
     3361 aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga gcgggcatca
     3421 acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc gtgcctcacc
     3481 agaacgtcac cgcgctgaat ctgcctggtc agtgcatcaa cggcgaatgc tatgacgaag
     3541 tactgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc gtgattgtct
     3601 tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg cagttcagga
     3661 aatttacccc aacctgcgac accaatgaga tccagacctg tagcaaagag caactggtga
     3721 acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg attaatctgc
     3781 tgaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac cacggtgaat
     3841 cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc ccggatagcc
     3901 aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaaacgg tatcaggttg
     3961 accagaactg cctgcgaaaa caggcgcaaa cgcaacacta ttcacaagac aatttattct
     4021 ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggctgcg gatgatattc
     4081 tgcaaacttg caggagagtg agtgaatgaa aattctgatt gttgaagacg atacgctgtt
     4141 attgcaggga ttgattctgg cggcgcaaac cgaaggctac gcgtgcgatg gcgtgacaac
     4201 cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg tactggattt
     4261 agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga aaaaatacac
     4321 cctgccagta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg ccgggctgga
     4381 tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac acgcccgtat
     4441 ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg ttggcaatct
     4501 gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga ttctgacgcc
     4561 caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg tgcatcggga
     4621 aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca ccctggaagt
     4681 gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg tgcgcggctt
     4741 tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcatt ttctgcgccg
     4801 accaatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt tggtgtttga
     4861 gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc tgtttgagca
     4921 ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc gcgaggcggt
     4981 cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta tctgctatca
     5041 ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg aagcgcgcac
     5101 cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg aagcggtggt
     5161 ttcggcgtta aacgatctgg tcagtcgcct gaccagcacg ctggataacg aaaggttgtt
     5221 taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt tgcatctgga
     5281 actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc ttgatcagat
     5341 gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat tttcttccgg
     5401 taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg acgaactcag
     5461 taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg ccgacatcac
     5521 cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag aaaacgccca
     5581 tcgttacagc ccgcaaggca gcaacattat gattaagatg caagaagatg acggagcggt
     5641 catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg agttgagtaa
     5701 agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa gtattgtcag
     5761 ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag agacttccgg
     5821 cacgcgggcc tgggtacggc tgaagaaaga tcagtacgtg gcaaaccaga tataaagaaa
     5881 gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac ggtttttcat
     5941 tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag tgaaccttaa
     6001 gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag cttattcatc
     6061 aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg caatctcgtg
     6121 gtcaatatca tcgattttct gctcgatatt atcgtaatgc tcgacgagaa tttccttcgc
     6181 ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg ccgtctcttt
     6241 catggttacg ccggtgatta aaccaaccac cgccactacc atcaggtaat aggcaggcat
     6301 catcagattc tgcgagcttt cgaccagcca ggccgccagc gttggcgtca gaccggcaac
     6361 cagcaccgaa atattaaatg ccgccgccag cgcgctgtat cggatatgcg tcgggaacat
     6421 cgctggcaag gtagaagcca taacgcccgt aaagcagtta aggatcaccg ccagcatcag
     6481 taacccggca aaaatcagac cgatgacgtt actgttaatc agaataaacg ccgggatcgc
     6541 caacacaaac agggcaacac taccaagtag cacaaacgga cgacggccaa aacggtcact
     6601 cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga taataatcag
     6661 caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca tataggtcag
     6721 caacatgtag taagtcacgt tggtggcaat aaccagacca atacatgtca acaggctgcg
     6781 ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca aaccttcgcg
     6841 gtcgccctgt tccagtttat caacatgctg ctgaaacgcc ggagtttctt ccagcgcatg
     6901 gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa tacgccagcc
     6961 ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca cgcccgcacc
     7021 cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc cacgtttacg
     7081 gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccgc cgaccgagaa
     7141 accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa tcgtgtcgta
     7201 ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag tgatagcgag
     7261 gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagcccgc caagcggtcg
     7321 aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc tggggtcagc
     7381 ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac caaaatcaaa
     7441 ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt taccgtcatc
     7501 aataatggtg acatcacgaa gggtaatggg ttttactttt ttccttttca gcatagcttt
     7561 cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg gccgcaaaag
     7621 gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg cttacaccct
     7681 ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt aatgaccttt
     7741 ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt ttaggactca
     7801 ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgag cattgccccc
     7861 gccagacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg ccagcgttgg
     7921 gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag ccaacttaaa
     7981 aaatcagcat gctccccgcc cgtcggaacg gtcgaagagg caaaccactg atggtaatag
     8041 tgacgcgtgg tcggtgccgc attgatactc tctgccagat gttgtgtcgc tttctcgagc
     8101 aaatcaccca gcaacaacgc caccgcttgc gggcttccca gacggcaggc atcgccaaat
     8161 gccccccagc gcaactcctc cagaacgata aaacagcgcc caggcaacga ccaggcgtta
     8221 tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc ctgcaccagt
     8281 aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa ccactcatac
     8341 aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg atccattttt
     8401 tgcagcagcg ccagatgctg cgcgatctgc gtcagtgcca gatgaccggg atccagcatc
     8461 gacgccagtt tttccaccaa tgtcagacgc aggctaagcg gcgcatttaa catgccgccg
     8521 ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcatcagtcg ttcacgaaac
     8581 tgctgctgat gggcttgtgg aaggcgggca tcatcaccct gcgccagatc cactaaaaag
     8641 cggggataaa ccgactccag cacgcgaccg gggccgtcaa gtaacgtctt ggtcaatatc
     8701 gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgggcgtcg gtgtaaatcc
     8761 agccgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc accgattcat
     8821 tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattccgtc aaacatgaag
     8881 cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc tgctctgcca
     8941 gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct cgttgcaaca
     9001 cactgtaatc aaccgtttct gtcggtgatt tctcatttcc ccgtccccag tcgggctgat
     9061 tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgccctgc tcgtccgttg
     9121 cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg ggaactacgc
     9181 cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat tccattttga
     9241 cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg gattccagct
     9301 cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt aacagtgtct
     9361 cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag gcggctaact
     9421 gcgcattcag cgcatcttgt tgttgacgca gaaagtggtt ggcggaggtc agggccagct
     9481 cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat agctgcaaac
     9541 tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt aacccgtgcg
     9601 cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta tgcgcagcag
     9661 atcgcagagc atacaacgag gcgttagcgt aagcggcatg aagcaacgcc tgaattggct
     9721 gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt tcgaggtccg
     9781 ccagatcgtc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct tccacccagc
     9841 gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc gcccgattcg
     9901 ccaggtagcc ccacatcgac gacaccggaa atatctgctg tggcgtaata cagcctttca
     9961 tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta cgatcctgtt
    10021 gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc aaaatcgcct
    10081 cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc gccagtaccg
    10141 ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc ccggcttcat
    10201 ttggcccggg ggtatccagt aacgtcaact gaccgggata actctccagc cccgccagat
    10261 ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca gaaaaaggaa
    10321 aatcgacgcc cagcgccttc gccagtcgca ctaaatcatt caaacttttc agacaatgaa
    10381 aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg atccgctgca
    10441 taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta atatcgcaat
    10501 cacgcaggcg ctgttgtaat tttttaatta aacaatcgat tggcgcgaca tgtgaaaaat
    10561 gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc gcagtcattg
    10621 ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg gttgatttcc
    10681 ctgctttcat ggtaccgaca atcgccagca ccatttccag tcgggaaatt ttacgcaact
    10741 cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt aaagccagtt
    10801 gtggcgcatc gtctccgggt acagagagag gcattttttc cagtaactgc aactgttggc
    10861 gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc tgtgtgtaca
    10921 tagaaaattc ttccttaaag caaattttat tacttttatt tagccagatt gtttttgagt
    10981 tctgtttt
//
LOCUS       DAOFQD010000158        10941 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.158, whole genome shotgun sequence.
ACCESSION   DAOFQD010000158 DAOFQD010000000
VERSION     DAOFQD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.158"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            63..5483
                     /locus_tag="QBF46_004177"
     CDS             63..5483
                     /locus_tag="QBF46_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HDQ6676290.1"
                     /translation="MNRIYRVIWNCTLQVFQVCSELTRRVGKKSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGGNENSKGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGF
                     GTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKEYSLVGVQDGSHGVINVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGFITNATKVEFGSGEGVFVINHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIANPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDMNVMPGG
                     ALRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5706..5972
                     /gene="hipB"
                     /locus_tag="QBF46_004178"
     CDS             5706..5972
                     /gene="hipB"
                     /locus_tag="QBF46_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDQ6676291.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPESTEQQNLEW"
     gene            5972..7294
                     /gene="hipA"
                     /locus_tag="QBF46_004179"
     CDS             5972..7294
                     /gene="hipA"
                     /locus_tag="QBF46_004179"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDQ6676292.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK"
     gene            complement(7580..8527)
                     /locus_tag="QBF46_004180"
     CDS             complement(7580..8527)
                     /locus_tag="QBF46_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676293.1"
                     /translation="MPRYKKGIRNNCFHQNYTHDVLFPGATFRTRHNGECAILGRSDD
                     KSRRGYYVVEFKDSGIIKEAYGSHIKTGSVSDEAFPSSEEERRKLLMTPKYYGVGYIG
                     NGCHSTIENTRTHQRTRAFILWHNMLARCYMTTKGKQYFKGYKGVTVCERWHNFQNFC
                     NDLPKLHGYNKWKDNPGEYELDKDYSHRRIYSADTVAFISTEENAREAGLRRVAMKIP
                     SGHYHEINKIRDEILMEAEDELKNNQINYEVVLDGNMKVILCETPYGTVLFWPLTKKI
                     QRNCYMIDGDVQVYVYYLRWLILQWENRNPDINCIATTC"
     gene            complement(9340..10431)
                     /locus_tag="QBF46_004181"
     CDS             complement(9340..10431)
                     /locus_tag="QBF46_004181"
                     /inference="COORDINATES: protein motif:HMM:NF018252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676294.1"
                     /translation="MKRLILAATVISASLLPFTCSANADSLGINKDCDNHPVSQYGMD
                     TITAFSQYKTESKKFKDALTAFCNKGKTMGDMGFEAANAHSLAEARKWTDSSLPGGNE
                     EQKKFSAAMGSLLHYAMLNGFSGFDYPRPPEQKEKPVSSFNPNEICDDISSKAAKYST
                     SNLPPQVQISLAEWNQIKGEFQMVCFAGIMAGNNRQSVDSKILAGMNDIGKEVYLSAY
                     RSGSEYPKLRSKNNTEKNDAAARKKEAAKEKADAAGKQSATNKTKQADDYKSRNVHLD
                     SPDFQRFQSSLRNEFMRRIGDAKRFSGKECSINVWMSQKGEVLSARKESGDEELCSVV
                     LQASKGMRLSPMSNEVYGVFRNPSLNFKF"
     gene            complement(10712..>10941)
                     /locus_tag="QBF46_004182"
     CDS             complement(10712..>10941)
                     /locus_tag="QBF46_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676295.1"
                     /translation="INSCLSQNLIPAYLQSQFTSLKTMLETGIPTIRNKNAGHGQGAD
                     IKEVPEELASYMLHLTATNLLFLLKCEKNIK"
BASE COUNT         2946 a         2347 c         2798 g         2850 t
ORIGIN      
        1 atttttaatg tctggcgact catctctttc gtggctgtat tgtttgtata tggagcgttt
       61 atatgaatag aatctatcgc gtgatatgga attgcaccct acaggtattt caggtctgct
      121 cggaattaac tcgcagggta ggtaaaaaat cgacggttaa tttgcgtaaa tcctctggac
      181 tgacaacgaa attcagtaga ttgacgctgg gtgttttgct ggcactaagc ggttcagcgt
      241 ctggtgcgag tctggaagtt gataatgatc agattaccaa tattgatact gatgttgctt
      301 atgatgccta cctggttggc tggtatggca ctggagtgct taatattttg gctggcggta
      361 atgcctcctt aaccacgatt actaccagcg tcattggtgg taatgagaac tcgaagggca
      421 ccgttaatgt tttgggtggc acctggcgat tgtatgatag cggaaataat gcaaggcctt
      481 taaatgtggg tcaatccgga acggggacgc tgaatattaa acagaaaggt cacgttgatg
      541 gaggatattt aaggctaggt tcctcgacag gaggcgtcgg gacggtcaat gttgagggag
      601 aggactctgt tctgacgacc gaattattcg aaatagggag ctatggcaca ggctcattaa
      661 atattacgga taagggttac gtcacgagtt caatagtcgc cattttaggc tatcaggcgg
      721 gcagtaatgg ccaagttgtc gttgaaaagg gcggtgagtg gctaataaaa aataacgatt
      781 cctcaattga atttcaaatt ggtaatcaag gaacggggga agcgactatt cgcgagggtg
      841 ggctgattac ggctgaaaac acgattatcg gtggcaatgc caccggtttc ggaaccctga
      901 atgtgcagga tcaagactct gtcatcacgg tacgcagact ctataatgga tatttcggaa
      961 atggcacagt caatatttcc aataatggac tgattaataa caaagaatat tcattggtgg
     1021 gcgttcagga cggttcccac ggtgtcatca acgtgaccga taaagggcat tggaatttcc
     1081 tcggaacagg cgaagctttc cgctatatct atatcggtga tgctggcgac ggtgaactta
     1141 atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac tgcggggatg aaagaaacag
     1201 gcacaggcaa cattactgtt aaggataaga actccgttat cactaatctc ggaactaatc
     1261 ttggttatga cggccacggc gaaatgaata tcagtaatga ggggcttgtt gtcagcaacg
     1321 gaggaagctc actcggttat ggagaaaccg gcgtcgggaa tgtcagcatc accacggggg
     1381 ggatgtggga ggtcaataag aatgtctata ccaccattgg tgttgcgggt gtcggaaacc
     1441 tcaatattag cgatggcggt aagtttgtat cgcaaaatat tacttttttg ggcgataaag
     1501 caagcggtat cggcactctg aacctgatgg atgcgacatc atcgttcgat actgtgggta
     1561 tcaatgtcgg taattttggt agcggtatcg taaatgtcag taatggtgcc acccttaatt
     1621 caacgggcta tggatttatc ggaggaaatg cctccggtaa ggggatagtt aatatttcaa
     1681 cggatagtct ttggaattta aagacgtctt ctactaacgc ccaattgcta caggtcggtg
     1741 tattaggcac gggtgaactg aatattacca ccggaggtat agttaaagcg cgtgatacac
     1801 agatagctct caatgacaaa agtaagggcg acgtgagggt ggatgggcag aactctcttc
     1861 ttgaaacatt caatatgtac gtagggacat ctggtacggg tacgttaacc ctgacgaata
     1921 gcggcacgct gaatgtcgaa ggtggagaag tttacttagg tgtttttgaa cctgctgtag
     1981 gaacgctaaa cattggtgca gctcacggtg aggcggcggc agatgccgga tttatcacca
     2041 acgcgacgaa agtagagttt ggctctggcg aaggtgtttt tgtcattaat catactaata
     2101 acagtgatgc cggctaccag gtcgatatgc tgattacagg tgacgataaa gacggaaaag
     2161 tgatccatga tgcaggccat acggtgttca atgcagggaa tacttatagc ggtaaaacgc
     2221 tggtcaatga cggcctcctg accattgcgt ctcatacggc agatggggta acgggcatgg
     2281 ggtcgagtga agtaaccatt gcaaaccccg gtacgctcga cattctcgca tcaacgaaca
     2341 gtgcaggaga ttacacgctg accaatgcgc tcaaaggcga tggtttgatg cgagtgcagc
     2401 tgtcatcctc cgacaagatg tttggcttta cccatgcaac agggactgaa ttcgccggtg
     2461 ttgcccaact gaaagacagt accttcactc tggaacgcga caacaccgct gcgcttactc
     2521 acgcgatgtt gcagtctgac agtgaaaata ccacatcggt aaaagtggga gagcaatcca
     2581 ttggtggact ggccatgaat ggcggtacgc tcattttcga tacggatatt cctgctgcga
     2641 cgcttgcaga gggatatatc agcgtcgata cgctggttgt cggcgcgggt gactacacct
     2701 ggaaaggccg taactatcag gtaaacggga cgggcgacgt gcttatcgac gtgcctaaac
     2761 cgtggaatga tcccatggcg aataaccctc tgacgacgct caatttgctg gaacacgacg
     2821 atagccatgt cggcgttcaa ctggtgaagg cgcaaacggt tattgggtcg ggtggctcat
     2881 taacgttacg tgatttacag ggcgacgagg tggaagcgga caaaacgtta cacattgcgc
     2941 aaaacggaac ggtggtcgcc gagggtgatt atggattccg cctcacgacc gcaccaggtg
     3001 atggtttgta cgttaactat gggctgaaag cgctgaacat ccatggtggg caaaagctga
     3061 cgttagccga acatggcgga gcctatggcg caacggccga tatgtcggca aaaatcggtg
     3121 gtgaagggga tctggcaatc aatacggtgc gacaggtttc gctttccaac ggtcagaacg
     3181 actatcaggg ggcaacctac gttcagatgg ggacattacg taccgatgcg gatggtgcac
     3241 ttggcaacac ccgggaactg aacatcagca acgcggccat cgtcgatctt aatggatcga
     3301 cgcagacggt agagacattc accgggcaga tgggttcgac tgttttgttc aaagaggggg
     3361 cgctgacggt aaataaaggt gggatcagtc agggtgaact gacaggtggc ggaaacctga
     3421 atgttacagg gggaacgctg gctatcgagg ggcttaatgc acgctacaat gcgttaacca
     3481 gcattagccc aaatgcggaa gtcagcctcg ataatactca ggggttaggc agaggaaata
     3541 ttgccaatga cggtctgtta acgctaaaaa acgtgactgg cgaactgcgt aatagcataa
     3601 gcgggaaggg tatcgtgagc gcaaccgcca ggacagatgt agagctggat ggcgataata
     3661 gccgctttgt ggggcaattc aacattgata caggcagcgc gctcagcgtc aacgagcaga
     3721 aaaacctggg tgatgcttcc gttatcaata atggcctgct caccatctcc actgagcgta
     3781 gctgggcgat gacgcacagt atcagcggta gcggtgatgt gacaaaactg ggtaccggga
     3841 tcctgactct taacaacgat tccgcggcgt atcagggtac gacggatatc gtgggggggg
     3901 aaattgcttt cggttccgac tctgccatta atatggcaag tcaacacatt aatatccata
     3961 acagcggtgt gatgtcggga aatgtcacca ctgcaggtga tatgaacgtt atgcctgggg
     4021 gggcactgcg tgtcgctaaa accactatcg gcggcaacct ggagaatggc ggcacggttc
     4081 aaatgaatag cgaagggggg aaaccgggga atgtactgac cgttaacggc aactataccg
     4141 gaaacaatgg cctgatgacg ttcaacgcga cgctgggcgg cgataattcg cccaccgata
     4201 agatgaacgt gaaaggcgat acccaaggga acactcgcgt tcgggttgat aacattggcg
     4261 gcgtcggtgc acaaacggtc aacggtattg aactcattga ggttggcggt aattctgcag
     4321 gtaacttcgc gctgaccacc ggaactgtcg aagctggggc ttacgtctac acgctggcta
     4381 aagggaaggg gaatgacgag aaaaactggt atctgaccag taaatgggac ggcgtaacgc
     4441 cagcggatac acccgatccc atcaataatc cccctgttgt ggatccggaa ggcccatcag
     4501 tttatcgccc ggaggccgga agctatatca gcaacattgc cgcagccaac tcgctgttta
     4561 gccatcgttt acacgaccgt ctgggtgagc cgcagtatac agattcactg cattctcagg
     4621 ggtcggcaag cagtatgtgg atgcgtcatg tcggagggca cgaacgttca agggccggtg
     4681 acggtcagct aaatactcag gctaaccgct atgtattgca gctaggcggc gatttggcgc
     4741 agtggagtag caacgcgcag gatcgctggc atcttggcgt gatggcaggc tacgccaatc
     4801 agcacagtaa tactcagagt aatcgtgtgg gttataaatc ggatgggcgc atcagcggtt
     4861 acagcgctgg gctgtacgcg acctggtatc agaacgatgc gaataagacc ggcgcttatg
     4921 ttgacagctg ggcgctgtat aactggtttg ataacagcgt cagttccgat aaccgttctg
     4981 ctgacgacta tgattctcgc ggtgtgacgg cctctgttga gggtgggtat acctttgaag
     5041 cgggaacatt tagcggcagc gaagggacgc tgaatacctg gtacgtccag ccacaggcgc
     5101 aaatcacctg gatgggtgtg aaagattccg accatacccg gaaagacgga acgcgcattg
     5161 aaacggaagg cgacggaaat gtgcaaacgc gacttggggt gaaaacctac ctgaatagcc
     5221 atcaccagcg tgacgatggt aaacagcgtg agttccagcc ttacattgaa gcgaactgga
     5281 tcaacaatag caaagtctac gccgtgaaga tgaatggtca aaccgtaggc cgtgaaggtg
     5341 cgcgtaatct cggtgaagta cgtaccgggg ttgaggcgaa agtaaataac aaccttagcc
     5401 tgtgggggaa tgtcggtgtg caactaggtg ataaaggcta tagcgatact cagggcatgc
     5461 tgggagtgaa atatagctgg taaaccgtat aagccgcatg tcgagatggc atgcggctta
     5521 atattgccga cttcaaacgg cgcatcaacg ccttatttaa atcctccttt ttatccgcga
     5581 tcgcggatat cgcagcgttt atcccgtaga gcggataaga tgtgtttcca gattgactta
     5641 tcctcactaa aggataaaac ttataatatc cccttaagcg gataaacttg ctgtggacgt
     5701 atgacatgat gagctttcag aagatctata gcccaacgca attggcgaat gcaatgaaac
     5761 tggttcgcca gcaaaatggc tggacgcaga gcgagctggc gaaaaaaatt ggtattaagc
     5821 aggcgacgat ttccaatttc gaaaacaacc ctgacaatac cacgctcacg acatttttta
     5881 agattttaca gtcgcttgaa ctctcaatga cgctatgcga cgcgaaaaat gcctcgccag
     5941 aatcaacaga acagcaaaat ctggagtggt aatgcctaaa cttgtcactt ggatgaacaa
     6001 ccagcgggta ggcgagttaa cgaagttagc caacggcgcg cacaccttta agtatgcacc
     6061 ggagtggtta gcaagccgtt atgccagacc gttgtcactt tcgctgccat tgcagagggg
     6121 gaatatcacc tctgatgccg tatttaactt cttcgataac ctgttacccg atagcccgat
     6181 tgtacgtgac cggatcgtta aacgttatca tgccaaatcc agacaaccgt ttgatttatt
     6241 gtcagaaata gggcgagaca gcgttggtgc cgtgacgtta atacccgaag acgaaaccgt
     6301 aacgcatccg ataatggcat gggaaaagct tactgaagcc agacttgaag aagtattaac
     6361 ggcttataaa gcagatatcc cgctaggcat gattagagaa gaaaatgact ttcgcatctc
     6421 ggttgctggc gcacaggaga agacagcact gctcagaata ggcaatgact ggtgcattcc
     6481 gaaaggaata acgccgacga cgcacatcat taaattaccg attggcgaaa tcaggcagcc
     6541 caatgcgacg ctcgatctca gccaaagcgt tgataatgag tattactgtc tgctgctggc
     6601 gaaagaactt gggttgaatg ttccggacgc agaaatcatt aaagcgggaa atgtgcgcgc
     6661 gttagcggtc gaacgttttg acaggcgttg gaatgctgag cgaacggttt tacttcgctt
     6721 gccacaggag gatatgtgtc agacattcgg tttaccttca tcggtgaaat atgaatcaga
     6781 tggaggccca ggcatcgcgc ggatcatggc ttttttgatg gggtccagcg aggcgctgaa
     6841 agatcgctat gattttatga aattccaggt cttccagtgg ttgattggcg caacggacgg
     6901 tcatgcaaaa aacttctccg tatttattca ggctggcggc agttatcgac tcacgccatt
     6961 ttacgacatc atttcagcat ttccggtcct tggcggtacg ggaatacaca tcagcgatct
     7021 caaactggca atggggctta acgcatccaa aggcaaaaaa acggcaatcg ataaaattta
     7081 tccgcgacat tttttggcga cagcaaaggt gctgagattc ccggaagtgc agatgcatga
     7141 aatcctgagt gactttgcca gaatgattcc agcagcactg gataacgtga agacttcatt
     7201 accgacagat tttccggaga acgtggtgac ggcagttgaa agcaatgtgt tgaggttgca
     7261 tggacggtta agccgagaat acggtagtaa gtgagatatg gggtttggtc attgttaatg
     7321 agcatgacaa tcatgaccgc caacgactgg attcttcctt ggtttatatt agtgcgcata
     7381 atgtgttgaa catgttaaat gactgcacga agtgctggca tttagccttc tgcaatgaac
     7441 tgccagacgt tgtcacttgt tttctggcat agaaaatcac tgcaacagcg gaattacaga
     7501 tacatcctga atgttcgttt ttcttattaa tagatagcat tgtgaaatgc cggttatttt
     7561 atgactattt atagcatcat caacaggttg tagcaataca gtttatgtcg ggatttctgt
     7621 tttcccactg aagtatgagc cagcgcaggt aataaacata aacctgtaca tcaccatcaa
     7681 tcatgtagca attacgctga atcttttttg tgagaggcca gaacaacact gtgccataag
     7741 gagtttcaca caggattact ttcatattcc cgtcaagaac aacttcataa ttaatttgat
     7801 tgttttttaa ttcatcttca gcctccatta atatctcatc tcgaatttta tttatttcat
     7861 gataatgccc ggaaggaatc ttcatggcaa cgcgtcgtaa tccagcttcc ctggcatttt
     7921 cttctgtgct gataaatgcc accgtgtcag cagaataaat ccggcgatgt gagtaatcct
     7981 tatcgagctc atactctccc ggattatcct tccatttgtt gtagccatgc aattttggaa
     8041 ggtcattgca gaagttctga aagttatgcc atcgctcgca tacagtgaca cctttgtagc
     8101 ctttaaaata ctgcttccct ttggttgtca tgtagcacct tgccagcatg ttatgccaga
     8161 gtatgaatgc tctcgttctt tggtgtgttc gggtgttttc aatagttgaa tgacatccat
     8221 taccaatata tccgacgccg taatattttg gtgtcatcag caactttcgt cgttcttcct
     8281 ccgatgatgg aaaagcttcg tctgatacag aaccagtttt aatatgcgag ccataggctt
     8341 ccttgatgat gccactgtct ttgaattcca cgacataata gcctcgcctt gatttatcat
     8401 cagagcggcc aagaatggcg cattctccat tatgtcgggt tctgaatgtt gcgccgggga
     8461 acaatacgtc atgagtataa ttttgatgaa aacaattatt acggatacct tttttgtagc
     8521 ggggcatgat atttctcctg cgtgatttaa ctgaaaggga agggagcaaa aagaatcggg
     8581 gcgggtcctt ctttttcgat tcaacttcag ccaggcactc atgccggaga gatggattat
     8641 atttatgtaa tcacttcaaa aaataaaata taaaattttt caaacaatga attgtgacag
     8701 atgatggtac aaaccgtcag gcgctctgtg tggcatcatt aatacggcac atacaaatgc
     8761 gtcaggttgc agccggatgc gttcagatgg ctgtacagat gcaataaaca gggcggaaca
     8821 gggggttgcc agtgtgagag ggcgcatatg cctgacaatt acagggctgt ttatttatac
     8881 agtatctggt attttgctta catcgtgacc gctgactgcc tgtatgcagt tttggggtga
     8941 ttttggcctt agtcatggct ttgcattacc actgactctg gcgttgcgca tggtgcatta
     9001 cagggagcgc actcagggtc aggactgcgc cggaatcacc cgatagggtg ggtaaataaa
     9061 aaaataaata taaccgtttt acagatttaa atatctgtgc taccatcgcc aaatcgccaa
     9121 ccggggttaa ttctcttata tattctgtta ttctcttata tagacttgct gtttaattgt
     9181 tattatcggt tttctgtact tatctgcatc tgtctttgat gtttcaatat tatcccaata
     9241 ttaagccctc cgcaggagag ccatgcggct tgagctgaca ataataacaa aaagaaaata
     9301 agaatcccca caaaagtgaa gactcttacc cggcatgaac tagaatttaa agttgaggga
     9361 tggattacgg aacactccgt aaacttcatt actcataggg ctaagcctca ttcccttcga
     9421 tgcctgcaat accacagagc aaagctcttc atctccggat tccttcctgg ctgacagaac
     9481 ctcacctttc tgtgacatcc agacatttat tgaacattct tttcctgaaa aacgctttgc
     9541 atcaccaatt cgccgcataa attcatttct gagtgaagac tgaaagcgtt gaaaatcggg
     9601 ggagtcaagg tgaacattcc tggatttata atcgtcagcc tgctttgttt tgttggtggc
     9661 actttgtttt ccggctgcat ctgctttctc ctttgcagcc tctttcttcc ttgctgcagc
     9721 atcatttttt tctgtgttgt ttttgctgcg aagctttggg tattccgaac cagaacgata
     9781 agcacttaaa tacacctctt tgccaatatc attcattcca gccaatattt ttgagtcaac
     9841 cgactggcga ttgttacccg ccataattcc cgcaaaacac accatctgaa actcgccttt
     9901 gatttgattc cactcagcta atgaaatctg tacttgaggg gggagattcg aggttgaata
     9961 tttggctgct ttagatgata tgtcatcgca tatttcattt ggattaaatg agcttactgg
    10021 tttttctttt tgctcgggcg gcctgggata gtcaaaacca gagaatccat tcagcattgc
    10081 atagtgaagc aggctcccca tggctgctga aaattttttc tgctcttcat ttcccccggg
    10141 aagactggag tcagtccatt tccttgcttc agcaagagag tgagcattgg cagcttcaaa
    10201 tcccatgtcg cccatggttt tgcctttatt gcagaacgca gtcagtgcat ctttaaattt
    10261 tttcgattcc gtcttgtact gactgaatgc ggtaatggtg tccatgccgt actggctgac
    10321 aggatggtta tcgcagtctt tattgatacc cagtgagtcg gcatttgccg aacaggtaaa
    10381 tggcagcaga cttgcactta ttacagtcgc agcaagtatt aatcgtttca tttgaatcag
    10441 ttcctgaggc gaacattcag aacactcaac aagcccagtg aaggccgcaa gacatttcaa
    10501 ccgttaggcg aaccaaccag actcagcaca tccatattct gcgagaacaa ggatgcccac
    10561 aaatcactgg attctccctt ggtttatgtt tgtcaaagct cagtgaaaat gtctaatcac
    10621 tggctcatta taaatgtcca tatgttgaaa aggccgctgc gaaaatccct cccgcagcgg
    10681 cctctttagc ataacgtcat tatgcgaacc atcacttaat gttcttctcg cattttaaga
    10741 gaaatagtaa atttgtagca gtaagatgaa gcatatagct tgccaattct tccggaacct
    10801 ctttaatatc agctccttgc ccatgacctg catttttgtt tctgatggtt ggaattcctg
    10861 tttccagcat tgttttcaga cttgtgaact ggctttgtaa atatgccgga attaaatttt
    10921 gacttaaaca gctatttatt a
//
LOCUS       DAOFQD010000159        10848 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.159, whole genome shotgun sequence.
ACCESSION   DAOFQD010000159 DAOFQD010000000
VERSION     DAOFQD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.159"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..452)
                     /gene="fliZ"
                     /locus_tag="QBF46_004183"
     CDS             complement(<1..452)
                     /gene="fliZ"
                     /locus_tag="QBF46_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDQ6676296.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTN"
     gene            complement(498..1217)
                     /gene="fliA"
                     /locus_tag="QBF46_004184"
     CDS             complement(498..1217)
                     /gene="fliA"
                     /locus_tag="QBF46_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDQ6676297.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1537..3324)
                     /locus_tag="QBF46_004185"
     CDS             complement(1537..3324)
                     /locus_tag="QBF46_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HDQ6676298.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSSLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGSGTIANKAATISDLTAAKMDAATNTITTTNNALTASKALDQ
                     LKDGDTVTIKADAAQTATVYTYNASAGNFSFSNVSNNTSAKAGDVAASLLPPAGQTAS
                     GVYKAASGEVNFDVDANGKITIGGQKAYLTSDGNLTTNDAGGATAATLDGLFKKAGDG
                     QSIGFKKTASVTMGGTTYNFKTGADADAATANAGVSFTDTASKETVLNKVATAKQGKA
                     VAADGDTSATITYKSGVQTYQAVFAAGDGTASAKYADKADVSNATATYTDADGEMTTI
                     GSYTTKYSIDANNGKVTVDSGTGTGKYAPKVGAEVYVSANGTLTTDATSEGTVTKDPL
                     KALDEAISSIDKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMS
                     KAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            3590..4996
                     /gene="fliD"
                     /locus_tag="QBF46_004186"
     CDS             3590..4996
                     /gene="fliD"
                     /locus_tag="QBF46_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDQ6676299.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            5021..5431
                     /gene="fliS"
                     /locus_tag="QBF46_004187"
     CDS             5021..5431
                     /gene="fliS"
                     /locus_tag="QBF46_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDQ6676300.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            5431..5796
                     /gene="fliT"
                     /locus_tag="QBF46_004188"
     CDS             5431..5796
                     /gene="fliT"
                     /locus_tag="QBF46_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDQ6676301.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLLLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            5874..7361
                     /gene="amyA"
                     /locus_tag="QBF46_004189"
     CDS             5874..7361
                     /gene="amyA"
                     /locus_tag="QBF46_004189"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDQ6676302.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(7395..7808)
                     /locus_tag="QBF46_004190"
     CDS             complement(7395..7808)
                     /locus_tag="QBF46_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6676303.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            7995..9200
                     /gene="yedE"
                     /locus_tag="QBF46_004191"
     CDS             7995..9200
                     /gene="yedE"
                     /locus_tag="QBF46_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDQ6676304.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            9197..9430
                     /gene="yedF"
                     /locus_tag="QBF46_004192"
     CDS             9197..9430
                     /gene="yedF"
                     /locus_tag="QBF46_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDQ6676305.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            9539..10210
                     /locus_tag="QBF46_004193"
     CDS             9539..10210
                     /locus_tag="QBF46_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDQ6676306.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(10195..>10848)
                     /locus_tag="QBF46_004194"
     CDS             complement(10195..>10848)
                     /locus_tag="QBF46_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6676307.1"
                     /translation="TPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARL
                     QRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVS
                     NMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYT
                     SQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGCQQTG"
BASE COUNT         2613 a         2746 c         2728 g         2761 t
ORIGIN      
        1 ttgttggtgc tggtggtggg cagccacggg gcgaggattt catcaaggaa accgtcctgc
       61 agcggatcga gggaaatatt ttgctcgtgt agatgattcc ccaaacggcg caaacgaacg
      121 acatattcac gcaccgtacc tgggctcatt tcagtttgct caaaaagaaa ttgcttaaag
      181 ccgataatat cgaaaaaatc actctgcgtt ttgcaatgta aatcaccgca aaatcggcac
      241 aatgccgccc atgatttttg ttccgtttgc cagccatttt catcaagcag cgcgtccagg
      301 cgggaaatct cgattttatt cactattttg ccgtcgcgaa cgagggtaat gcgatcgagt
      361 aatttacggc aatgcgcgca atgagtctgg ctgtgtttaa agtctttaag atagcggctt
      421 aatggccgtc ttttcaggtg ctgcaccatc attaagaact cctggtagtc aaaattaaag
      481 tgcggcattt actgacgtta taacttaccc agtttagtgc gtaaccgttt aatggcctgg
      541 ctgtgtaact gactgacccg cgattccccg acctccagca ccgcgccaat ctctttgaga
      601 ttcagctctt cctggtaata gagggttaat accagttttt cgcgctccgg caacgtttcg
      661 atagcttcca tcacccgctg gcgcagatta ctgtccagca gttgttgtag cgggttttct
      721 cgctgatgat catcagtaac cagttcgatg ctatcgccgt gctcttcgcg ccactcatcg
      781 taggagaaga gctggctgtt attggtgtcg agcaacattt ggcgataatc ggcaatatcg
      841 atccctaaac gttccgctac ctcagtttcc gtggcgttgc ggccaagttc ctgctccagt
      901 tgccctattg cctgtgccac ttcacgcgcg ttgcgtcgca cgctgcgcgg cacccagtca
      961 cggctgcgaa gttcatccag catagcgcca cggatacgct gcactgcgta agttgtaaat
     1021 gccgttcctt gtagggcgtc atagcgttcg acggcattaa gtaacccaat gccgcccgcc
     1081 tgtagcagat cgtcaagttc cacgctcgcg ggcagtcgaa cctgcaggcg caatgcttcg
     1141 tgacgcacca gcgggacata acgctgccac agcgagtgtt tatccattac accttcagcg
     1201 gtatagagtg aattcacgat aaacagccct gcgttatatg agttatcggc atgattatcc
     1261 gtttctgcag ggtttttaat cggacgatta gtgggtgaaa tgaggggtta tttgggggtt
     1321 atcggtaaat tgcgggcaga aaaaaccccg ccgttggcgg ggaagcacgt tgctgacaaa
     1381 ttaccattca tgttgccgga tgcggcgtaa acgccttatc cggcctacaa aaatgtgcaa
     1441 attcaataaa ttgcaattca ccttgtaggc ctgataagcg cagcgcatca ggcaatttgg
     1501 cgttgccgtc agtctcagtt aatcagttta cgacgattaa ccctgcagca gagacagaac
     1561 ctgctgcggt acctggttgg cttttgccag cacggagtta ccggcctgct gaatgatctg
     1621 cgctttcgac atgttggaca cttcggtcgc atagtcggcg tcctgaatac gggactgcgc
     1681 ttcggacagg ttggtagtgg tgttgttcag gttggtgact gcggaatcca gacggttctg
     1741 gatagcaccc agggaagaac ggaatttgtc gatggagctg atagcttcat ccagagcttt
     1801 cagtggatct tttgttactg tgccttcgct agttgcatct gttgttaaag taccattagc
     1861 actaacatat acttcagccc ctactttcgg cgcatattta cccgtaccag ttccagaatc
     1921 aacagttacc ttgccgttgt tagcatcgat tgaatacttc gtggtgtatg aaccaattgt
     1981 agtcatttca ccatcagcat cagtgtatgt tgctgttgca ttagaaacgt cagctttatc
     2041 ggcatatttt gcgctagcag taccgtcacc tgcggcaaat acagcctgat acgtctgaac
     2101 gccagattta taggtaattg ttgcggatgt atcaccgtca gctgcaactg ctttgccttg
     2161 tttagctgta gccactttat ttaaaacggt ttctttgcta gctgtatcag tgaacgatac
     2221 ccctgcgtta gcagttgcag catcagcatc agcacccgtt ttaaagttat aagttgttcc
     2281 ccccatcgtg actgatgcag tcttcttaaa cccgattgat tgaccatcac cagctttctt
     2341 gaataaacca tcaagcgtag ccgcagtcgc accaccagca tcgtttgtag ttaagttacc
     2401 atcactagtt aaatatgctt tctgtcctcc gattgtgatt ttaccattcg catcaacatc
     2461 aaagttcact tcaccgcttg ctgctttata aacaccacta gcagtttgcc cagccggcgg
     2521 gagaaggcta gctgctacat cacctgcttt tgctgaagta ttattcgata cattactgaa
     2581 tgagaagtta ccagctgatg cattgtatgt ataaaccgtg gcagtttgag cagcatctgc
     2641 tttgatagta acagtgtcac catctttcag ttgatcaagc gcctttgatg cagtcagcgc
     2701 attatttgtt gtagttatag tattagttgc agcatccatt ttcgctgctg tcaggtcgct
     2761 aatggtcgcc gctttattgg ctatcgtacc ggaaccattc acgttaaaac cattcagccc
     2821 cagcgtatct gagtcaattt tcttcagatc aatcgtgata gtctggccgt cattcgcacc
     2881 aacctgaatt ttcattgaac cgtctttcgc cagtacgttc acgccgttga actgggtctg
     2941 gccagatacg cggtcaattt cgtccagacg ggatttgatt tcgtcctgaa tggagtccag
     3001 atccgaatcg gagttagtcc cggtagaagc ctgaaccgtc agttcacgga tacgctgtaa
     3061 gttgttgttg atttcggaca gcgcgccttc ggtggtctgc gcaacggaga taccgtcgtt
     3121 ggcgttacgg gccgcctgag tcaggccttt aatgttagag gtgaaacggt tagcaatcgc
     3181 ctgacccgct gcgtcatcct tcgcgctgtt aatacgcaag ctagaagaca gacgctcgat
     3241 agaactcgac agcgcagact ggttcttgtt gatattattt tgagtgatca gcgagaggct
     3301 gttggtatta atgacttgtg ccatgattcg ttatcctata ttgcaagtcg ttgattacgt
     3361 attgggtttc cacccgtcgg ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca
     3421 acctttagaa tttttttcaa aaacagccat tttttgttag tcgccgaaat actcttttct
     3481 ctgcccctta ttcccgctat taaaaaaaac aattaaacgt aaactttgcg caattcaggc
     3541 cgataacccc ggtattcgtt ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat
     3601 ttcatcgctg ggagtcgggt caggtctgga tttaagttca atccttgata gcctcaccgc
     3661 cgcgcaaaaa gcgacgctaa cccccatttc aaatcagcaa tcgtcgttta ccgctaaact
     3721 tagcgcctac ggtacgctga aaagcgcgct gacgactttc cagaccgcca atactgcatt
     3781 gtctaaagcc gatctttttt ccgccaccag cactaccagc agcaccaccg cattcagtgc
     3841 caccactgcg ggtaacgcca tcgccgggaa atacaccatc agtgtcaccc atctggcgca
     3901 ggcgcaaacc ctgaccacgc gcaccaccag agacgatacg aaaacggcga tcgccaccag
     3961 cgacagtaaa ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag
     4021 cgcggctaac tcatcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcaggcgt
     4081 aagcgcaagc atcattaacg tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga
     4141 caccggcctt gacaatgcga tgacactctc ggtcagcggt gatgatgcgc tacaaagttt
     4201 tatgggctat gacgccagtg ccagcagcaa cggcatggaa gtctcggtcg ccgcccagaa
     4261 tgcgcagctg acggtcaaca acgtcgccat tgagaacagc agcaacacca tcagcgacgc
     4321 gctggaaaac atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctgaccat
     4381 cactcaggac acctccaaag cgcaaacggc gattaaagac tgggtgaatg cctataactc
     4441 gctaatagat accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag
     4501 ccaaagctct agcaatggcg cactgctcgg cgactccacg ctgcggacga ttcagacgca
     4561 gttgaaatcg atgctgagta ataccgtcag ttcttccagc tataaaacgc tggcgcagat
     4621 tggtatcacg accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc
     4681 tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac
     4741 cggcatcaca accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat
     4801 taaagccgct accgatggcg ttagtaagac cctgaataaa ttaactaaag actacaacgc
     4861 cgccagcgat cgcattgatg cgcaggtcgc gcgctacaaa gaacaattta cccaactgga
     4921 cgttttaatg acctcgttaa acagcacaag cagctactta acgcagcagt tcgaaaacaa
     4981 tagtaattcc aagtaatcat tattcatcgg gagacaggtc atgtacgcgg caaaaggcac
     5041 ccaggcctat gcacaaattg gcgtcgaaag cgccgtaatg agcgccagcc agcagcagct
     5101 ggtcaccatg ctatttgatg gagtgctgag cgcactggtt agagcgcgcc tgtttatgca
     5161 ggataacaat cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa
     5221 cggactgcgg gtgagtcttg atgaagagag caaagacgaa ttaacccaaa acttgattgc
     5281 tctttatagc tatatggtca ggcgcttgct gcaagccaat ttacgcaacg atgtctccgc
     5341 agtcgaagaa gtggaagcat taatgcgcaa tattgccgat gcctggaaag agtcgttact
     5401 ctccccttct ttgattcagg acccagtctg atgaaccatg caccgcattt gtatttcgcc
     5461 tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg
     5521 gacgaactca tcgccagcga aatggcgtat gtgaatgcgg tgcaggagat tgcacatttg
     5581 actgaagagg ttgacccgtc caccacgatg caggagcagc tccgcccgat gctgctcctg
     5641 attctcgaca acgaaagcaa ggtaaagcag ttattacaga ttcgtatgga tgaactggcg
     5701 aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc
     5761 ggctttgtgc tggctccgca agataacttc tcgtgaatct taacgagcta atggctcgcg
     5821 aataatccga ttacggctac gcttcaaatg ttccccttga atggagtcga agaatgcgta
     5881 atcccacgct gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg
     5941 aactggccga gcgcgccgac ggttttaatg atattggtat caatatggtc tggttgccgc
     6001 ccgcctataa aggcgcatcg ggcgggtatt cggtcggcta cgactcctat gatttatttg
     6061 atttaggcga gtttgatcag aaaggcagca tccctactaa atatggcgat aaagcacaac
     6121 tgctggccgc cattgatgct ctgaaacgta atgacattgc ggtgctgttg gacgtggtag
     6181 tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg
     6241 atgaccgtac gcaaattgac gaagaaatca ttgagtgcga aggctggacg cgttacacct
     6301 tccccgcccg tgccgggcaa tactcgcagt ttatctggga tttcaaatgt tttagcggta
     6361 tcgaccatat cgaaaatcct gatgaagatg gcatatttaa aattgttaac gactacaccg
     6421 gcgaaggctg gaacgatcag gttgatgatg aactcggtaa tttcgattat ctgatgggtg
     6481 agaatatcga tttccgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg
     6541 tgatggaaca aacacaatgc gacggttttc gtcttgatgc ggtcaaacat attccggcgt
     6601 ggttttataa agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg
     6661 tggcagagta ctggtcgcat gaagtcgata aactgcaaac gtatattgat caggtggaag
     6721 gcaaaaccat gctgttcgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg
     6781 gacgcgacta cgacatgacg cagattttca ctgggacgct ggtggaagcc gatccttttc
     6841 acgccgtaac cctcgttgcc aaccatgaca cccaaccgtt gcaagccctc gaagcgccag
     6901 tcgaaccgtg gtttaaaccg ctggcgtacg ccttaatttt gttgcgggaa aatggcgttc
     6961 cttcggtatt ctatccggac ctctacggtg cgcattacga agatgtcggt ggtgacgggc
     7021 aaacctatcc gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc
     7081 agcgtttcgc ccacggtgta cagacgttat ttttcgacca tccgaactgc attgccttta
     7141 gccgcagtgg caccgacgaa tatcccggct gcgtggtggt catgtcgaac ggggatgatg
     7201 gcgaaaaaac cattcattta ggagagaatt acggcaataa aacctggcgt gattttcttg
     7261 ggaaccggca agagagcgta gtgaccgacg aaaacggcga agcaaccttc ttttgcaacg
     7321 gcggcagcgt cagcgtgtgg gttatcgaag aggtgattta aattcatccc cggcggcaag
     7381 ccggggagat ttcattacgg cagtggcgtc ggtaacggcg ctttatccag cgcggcggca
     7441 cactctgccg tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatagtgtta
     7501 ccgtcacgct caacctcgta cagctcacgt tttacggtaa cgttagtgag gtcatcagac
     7561 atcaatgtca acttacccgg cacggcaatc acccgctgcc actgacggca atccagggta
     7621 tcgccctctt tagtgacaat caggctgcct atcgcttccg ggctgaccag cgcacgttgc
     7681 ggccctttgg tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg
     7741 ttatagtttt cgacttcggc acagccagcc agcaacatga gcgcgcctgc aattgcgagt
     7801 tttttcatca ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gccctgatgt
     7861 cgccagcctt tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta
     7921 ccccgaaaat aaatttgcat gatgtatgat tcgcgactct tgtttcaaac cttctgagtt
     7981 cagaggctac attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg
     8041 cacccatccc tgcggtcatc gcggcgggta ttctctccac ttactatttt ggcattactg
     8101 gcactttttg ggctgtcacg ggtgaattta cccgttgggg cggtcagctc ctgcagctgt
     8161 ttggcgtcca tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat
     8221 taacccgcat cgacgggatg atgatcctcg gcatgtttgg cggctgcttt gccgcagcgc
     8281 tgtgggccaa caatgtcaaa ctacgcatgc cgcgcagccg tatccgcatt atgcaggcca
     8341 ttattggcgg cattatcgcc ggttttggcg cgcgtctggc aatgggctgt aacctggcgg
     8401 cgttctttac cggcattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg
     8461 ccattggttc atggtttggc gcacgcttta ccctgctgcc catcttccgt attcccgtga
     8521 aaatgcagaa agtttctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc
     8581 gttttcgtct cgggatgctg gtctttttcg gtatgctggg ctgggcgctg ctcacggcga
     8641 tgaaccaacc aaaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg
     8701 aacgtgcgca aatctgcttt acttcggcgt tccgcgatat gtggatcacc ggacgtaccc
     8761 atatggcgaa agcaatcatt atcggtatgg cggtgagtgc catcgggatc ttcagttacg
     8821 tacagttagg cgttgaaccc aaaatcatgt gggcgggacc aaacgcggta attggtggtt
     8881 tactgtttgg ttttggcatc gtgctggcag gcggctgcga aaccggctgg atgtaccgcg
     8941 cggtagaagg ccaggtgcac tactggtggg tcggtctggg caatgtgatc ggctcgacga
     9001 ttctggcgta ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca
     9061 acctgctgaa aacctttggc ccgatgggtg gcctgctggt gacatatttg ctgttgttta
     9121 ctgcgctgat gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga
     9181 ctgctaagga gatcgcatga aaaatatcat tcctgattac cgtcttgata tggtgggtga
     9241 accctgccct tatccggcag tcgcaaccct tgaggcgatg ccgcagttaa aaaaaggtga
     9301 gatcctggaa gtggtgagcg actgtccgca gtcgatcaat aatattccac tggatgcgcg
     9361 taatcacggc tatacggtgc tggatattca gcaagacggg ccaaccattc gttatttaat
     9421 tcagaagtaa tccctcattc ccgctgtggc caacagcggg aacatcgtta cctctgtatc
     9481 acatagattt tacccgcccc ctctcctatt cttttcagta tgatctcagg aggttgctat
     9541 gtgtggacgc tttgcccaat cccaaacgcg tgaagattac cttgcgcttc tcgcggaaga
     9601 tattgaacgc gatattccct acgatcccga acccattggc agatacaacg tcgcgccggg
     9661 aaccaaagtt cttctgctca gtgaacgtga tgaacacctt catctggatc cggttttctg
     9721 gggatatgct cccggatggt gggataaacc gccgctgatt aacgcccgcg tagaaactgc
     9781 agccaccagt cgtatgttta aaccgctctg gcaacatggt cgggcaatct gttttgccga
     9841 tggctggttt gagtggaaaa aagaaggcga caaaaaacag ccttatttta tctatcgcgc
     9901 tgatggacaa cctgttttta tggccgcgat aggtagcaca ccctttgagc gcggtgacga
     9961 agccgaagga tttttaattg tcactgcagc agcagaccag ggtctggtag atattcatga
    10021 ccgccgcccg ctggttctgt cgccagaagc tgcacaggaa tggatgcggc aagagattgg
    10081 cggtaaagaa gcatcagaaa ttgctaccaa cggctgtgtg ccagcaaacc agttcacctg
    10141 gcacccggta tcgcgcgcgg taggtaatgt caaaaaccag ggcgcggagt taattcaacc
    10201 tgtttgttga catcctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc
    10261 gccatttaca tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga
    10321 caggcggttc tctttcgctg tatgaccaca gtacgcgcaa cgctggcttg tatacgctgg
    10381 cggaacagca agcacctgac cgccacgcca gagctgcttg tactcaagct ggcggtgcat
    10441 ttcataccag ccctgatcca gtatcgaacg gtttaaacct gattttgccc ggacattgcg
    10501 ccccggctgg ctgaccgtac ccgctgctga ctttgacatg ttgctgacct tcaaatcctc
    10561 aatgactatc attgcgtggt ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct
    10621 gcggatattt gcgatacagg aatgcagtcg ctgtattttg cgtttctgct tctgccagtt
    10681 gttgctgaat ttgaccttgc ggcttaactg tcgctgaagt ctcgccagcg tcttctggtt
    10741 tttctgaaaa ctgtttacag gctcaaagac tgtgccatct gacagcgtgg cgagtttagc
    10801 cacgccagca tccagcccga ccattgatgc tgaagggtga gccggagt
//
LOCUS       DAOFQD010000160        10794 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.160, whole genome shotgun sequence.
ACCESSION   DAOFQD010000160 DAOFQD010000000
VERSION     DAOFQD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.160"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(68..487)
                     /locus_tag="QBF46_004195"
     CDS             complement(68..487)
                     /locus_tag="QBF46_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6676308.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQIRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            567..821
                     /locus_tag="QBF46_004196"
     CDS             567..821
                     /locus_tag="QBF46_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6676309.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            818..1240
                     /locus_tag="QBF46_004197"
     CDS             818..1240
                     /locus_tag="QBF46_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676310.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQDALSTAIDAHDDAVQAVYRKAHFSGGGSPSDSVVVH"
     gene            1318..2106
                     /locus_tag="QBF46_004198"
     CDS             1318..2106
                     /locus_tag="QBF46_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676311.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVETNGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTKTDQKALPEEEKEEDINTYLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            2113..2859
                     /locus_tag="QBF46_004199"
     CDS             2113..2859
                     /locus_tag="QBF46_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ6676312.1"
                     /translation="MKNIAAGGILERIRRLAPPHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCQVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            2882..3643
                     /locus_tag="QBF46_004200"
     CDS             2882..3643
                     /locus_tag="QBF46_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HDQ6676313.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAPAELPEETTTATVAKISESDLTATIEQRGPLTADELATL
                     FGTTSRKVASTLAMAISKARLIRVNQNGKFRYCMPGGNLPAEPKAASVAETDGKAFPQ
                     LAGVALPVQEAATQEDIKTETVADIVQSLPSFTETQADNLILPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            3659..4081
                     /locus_tag="QBF46_004201"
     CDS             3659..4081
                     /locus_tag="QBF46_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDQ6676314.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRILSFYQGDVRYLLK"
     gene            4422..6419
                     /gene="betT"
                     /locus_tag="QBF46_004202"
     CDS             4422..6419
                     /gene="betT"
                     /locus_tag="QBF46_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDQ6676315.1"
                     /translation="MSENDTTPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPIGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLAVLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGVVAIALLIAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDIIQPAMTLVQEELNKQG
                     TISHISDAAEDRIRLEVDLGNELNYIYEVRLRGYNSPTFALAALDNDEQQSEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            6483..7760
                     /locus_tag="QBF46_004203"
     CDS             6483..7760
                     /locus_tag="QBF46_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HDQ6676316.1"
                     /translation="MISRWKWMLKQTFKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAKVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYVDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSTPVLYLSQGQESSISNDLLETIIVSDVRSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSAYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPGIFAEAAQTLAFETL
                     EHATRADHIDSDRYLIKSIYYNLFSGEDSNKKP"
     gene            complement(7891..8772)
                     /locus_tag="QBF46_004204"
     CDS             complement(7891..8772)
                     /locus_tag="QBF46_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6676317.1"
                     /translation="MSHLSHLRTFLEAYRSGSFSKAAELLGITQPAASQHIQSLETLV
                     GKRLFIRQARGVTATEAADELARSVSPFIDGLEEKLSSFRSGSVRGGTINLAGPPDFI
                     HSRLGTVMAPLMKEGYRLRFHTGNKQRIYSLLHDGSADLAVTASMPDDRLYGYAHLLT
                     ERMLLVHAPAMSEIIGSEPGAETLASLPLIAYDEELPLIRALWASMFQRAPEMQATFT
                     IPDLRIIRDLVCDGHGWTVLPDYHCDNDLKSGRLISVTPADLAPTNHLYLVWNKRTAN
                     NSRTAYVRDYILKTFPR"
     gene            complement(8769..9461)
                     /locus_tag="QBF46_004205"
     CDS             complement(8769..9461)
                     /locus_tag="QBF46_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003916403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDQ6676318.1"
                     /translation="MLTRENMLNGTFVRVHEALLPANMLWDAEKIRASMNSTLEERPT
                     GAPVWIFAYGSLMWNPVLAIEEMQRARLKGWQRSFCIRLISGRATPDVPGRMLSLDEG
                     GITEGIAFRLPESTLINELEIVWTREMITGLYRPVWADVSLGNGEEIQALAFVSDRLH
                     PHYEQDNLTATVALSIASARGDIGTNADYVWKLQATLGALGIRDDYVSELALALHEAP
                     CNDKLHQRGASL"
     gene            complement(9473..10672)
                     /locus_tag="QBF46_004206"
     CDS             complement(9473..10672)
                     /locus_tag="QBF46_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6676319.1"
                     /translation="MPFVIYVFTISAFALGLAEFVPIGLTDVMAQGLCVGVEQTGAAV
                     TTYALGATFAAPVLSALTASWSRKNVMLTTAVVFTAGSLAAAFAATLPQLLLARFIAG
                     LGHGLFLAAASSTAARLAGPGKAGRAVAVVFGGFTLAMAIGVPLSTWLGGVVSWRPVL
                     GAIAAFGAFGFLGLLFGMKDPVRSVPGEHSDSAMQNIGMLFNRKLLAGALVTVLAYAG
                     SFTAYTFIAPILTQVTGVTGATVSLFMLVYGITAAVGNILGGKLTDSMGVNRANILII
                     SGIVVVVLGMWLLSSSPVSMGILVALLGMVTFAAVPALQARLIGVAEQHAPHAHGVAA
                     GLNIAGFNSGIALGSVLGSITISHAGIVYTGISGAIVSLLGLLLLLAQIRRSHSRKFQ
                     QVKIVGQ"
BASE COUNT         2838 a         2580 c         2737 g         2639 t
ORIGIN      
        1 taaaaaaatg agacgggata aagtgaagca caaacccgga gggggacgct accggattta
       61 tgctgattta agaggctttt tgttttttct ttcgtgctaa ctcttcgtaa attgcattgt
      121 acttctgttt tttctcctca agagttttta aaagttcatc tgtctcactg tcagggagct
      181 cgtccagaag gtcaatgatg attttttgtc ttggatttaa ctcctgatag aaacgtatct
      241 gtccactttc ttctgtatcc tctcccaaaa gataggttgg tgttgttcca atgagtgttg
      301 ctaattccct taatttctct cggcgaggaa ttgtttcacc attaaaccat ttgctaaccg
      361 cttttggtgt taatttcatt cgacgggcaa tttctgcctg ccttccatgt tgttcataac
      421 cagcgttttc acaggctagc gcaagcctac tggcgaactc tttacgcgct ttatcttcat
      481 gaaccataag ttcaatgata ttcgctcttg aatgtactgt cagttctgtt atggcatgta
      541 ctcaaagttc acattgtgag gatgatatga accagaaaac acttgaagat gtaatcaaaa
      601 ctgttcgcgt ttctgttgtg gccgacgttt gtggtgtcag ccaaagagca atctacaaat
      661 ggatggataa cggaaaattg cctcgcacag aatataccgg cgaaacaaat tacgctgaaa
      721 aaatcgctca tgcatcaaac ggattatttt ctgccgatgc aattttaact attggcagaa
      781 ataaaactac tacgaaaaag ctgatgggag ttgattcatg aaaatcaagc atgagcacat
      841 cgaatcagtg ttgttagccc tggcagccga aaaagggcag gcgtgggtcg ctaacgcaat
      901 tactgaagaa tatctgcgcc aggggggcgg cgaattgccc ctggtaccag gcaaggactg
      961 gaacaatcag cagaatatct atcatcgttg gttgaaaggt gaaacgaatg cgcaaaggga
     1021 aaaaattcag aaactgatcc ctgcggttct ggcaattctt cctcgcgagc tgcgtcaccg
     1081 actctgcatc ttcgataccc tggaacgccg tgcattactg gcggcacagg atgcactgag
     1141 tacggcaatt gatgcgcatg atgatgcagt ccaggccgtt taccgtaaag cacatttcag
     1201 cggcggcggt tctcctagcg attctgtcgt agtgcattga ttgaaattaa tcgtaccgaa
     1261 ctgttttgtt cggtatcagt taaatgtaac gctgcgagcg ttacaaggtg aaaacaaatg
     1321 gcttcaaact ggataaagct cgaagttatt acgccggata agccggaaat attcaggctt
     1381 gctgagattc tgaatattga tccagatgcc gcattaggga aagtcattcg cttctgggca
     1441 tgggcggatc aacaaatgat agacggtaac gcagagtgta acgctcgcgg cgttacaaaa
     1501 agtgcaatag atcgcatcac ttttatggct ggttttgctg atgcgttaat tcaggttgga
     1561 tggctggtcg aaactaatgg tgtgttgtcg cttcctaact ttgagcgcca taacgggaaa
     1621 agctctaaaa aacgggcggt tacaaacgag agagtaacaa aaatacgaga actgaaacga
     1681 aaaggtaacg ctgccagcgt tacaaaaacg gatcaaaaag cgttaccaga ggaagagaaa
     1741 gaggaagata taaatactta tctcccccta aatccccctc gccaaaaacg agcgtctaaa
     1801 aaattcgaac cggaggctat cgagctgcct gactggttgc cggaaacact ctggcatgag
     1861 tgggttcagt tcaggcaggc attgcgaaaa ccgattcgaa cggagcaggg cgctaacggg
     1921 gcgatacggg agctggaaaa attccgccag cagggttttt cacctgagca ggtgattcga
     1981 cacagcatcg ccaatgaata ccagggcttg ttcgcgccaa aaggtgttcg acctgagacg
     2041 ttactccgac aggttaacac cgtctcgtta ccggatagtg cgatcccgcc aggcttcagg
     2101 gggtaactga ccatgaaaaa tattgcggca ggcggcattc ttgaacgtat ccgcagactg
     2161 gccccgccac atgtaaccgc cccattcaaa acggtagcgg agtggcgcga gtggcaactt
     2221 tccgaaggcc agaaacgttg tgaggagatc aaccgtcaga atcgtcagtt gcgggtggaa
     2281 aaaattctga atcgctctgg catccagcca ttgcaccgca aatgctcgtt ttcgaattac
     2341 caggtgcaga acgaagggca gcgatacgcg ttgagtcagg cgaaatccat cgctgatgaa
     2401 ctgatgaccg ggtgtacaaa ttttgcgttc agcggaaaac ctggtaccgg aaaaaaccat
     2461 ctggcggcgg ctatcgggaa tcgcctgctg aaagatggcc agacagtgat tgtggttacc
     2521 gtggctgatg tcatgagtgc cctacacgcc agctatgacg acgggcaatc aggcgaaaaa
     2581 tttttgcggg aactgtgcca agtggatctg ctggttcttg atgaaattgg cattcagcgc
     2641 gagacaaaaa acgagcaggt ggtgctgcac cagattgttg atcgccggac agcgtcgatg
     2701 cgcagcgtgg ggatgctgac aaacctgaat tatgaggcca tgaaaacatt gctcggcgag
     2761 aggattatgg atcgcatgac catgaacggc gggcgatggg tgaattttaa ctgggagagc
     2821 tggcgtccga atgtcgtcca gccaggaatt gcgaagtaat ttttaccggg agaaaaattt
     2881 aatggagact gtttttgacg cactgaaagc aatgggaaaa gccacatcca tagaacttgc
     2941 tgcgcgactt gatatcagcc gtgaagaagt gctgaacgaa ctatgggaac tgaaaaaggc
     3001 tggtttcgtt gataaaagcg cgtacacctg gcgtgtggct gataacaacg ttcagcagga
     3061 acagccagcg ccggcagaac tgccggaaga aactaccacg gcaacagtcg cgaaaatttc
     3121 agagagcgat ttaactgcga cgattgaaca acgtggtcca ctaactgcgg atgaactggc
     3181 tacgttgttc ggtaccacat cccgcaaagt ggcttcaacg ctggcaatgg caatcagcaa
     3241 agctcgtctg attcgcgtta atcagaacgg taaatttcgt tactgcatgc cgggcggtaa
     3301 tttaccagca gagccgaaag ctgcatcggt agcggaaaca gatggtaaag cctttcctca
     3361 gctagccggt gttgcgttac cagtacagga agctgcaaca caggaagata ttaaaacaga
     3421 aactgtggcg gacattgtgc agtcgttgcc atcgtttact gaaacgcaag cggataacct
     3481 gattttacca tcgctgcata tggcaaaccg tgaactgcgt cgggcgaaaa atcatgtcca
     3541 gaagtgggag cgagtctgcg ccgcgctgcg ggagctgaac aagcaccggg atattgttcg
     3601 acagattact gattcttccc gccgtgttgt atcggaaaag tgattgccgg aggcgcttat
     3661 ggcaaaagta tttacacaag aagagcggga aaaaattaaa gggcaggttg ttgaactagt
     3721 acgccggagt gggcgcgaga cgttacggca actggaagcc aagacaggtg cgacaagata
     3781 tctgatgagc gttctcgcca gagagctggt tgccagtggc gatgtataca actctggtta
     3841 cgggttattc ccgtctgaac aggctcgtaa ggactggcaa aatgcccgca aaaaactatc
     3901 tagggcaaag ctgaagaaac cggttgtggt tgatccggac cttatctggt cattacctga
     3961 cggagaaata cgtcgctacg acagtcgcct aaacataatc tgtcgcgagt gccggaagag
     4021 cgaagttatg cagcgcatat tgtcgtttta tcagggggat gttcggtatt tattgaagtg
     4081 acgagattaa agtgcattag ttcagatgca aattgacatt ttgtggcaca gggtagagct
     4141 agcgtggttg tccgctttgt gccaagagca gacgctggta attcgtgcct aatatacttc
     4201 acttaactta tatcccagct ttgatacaga tcatacaaat taaatgcccc agtaattggc
     4261 aggctgagta acttaaaact ccttagaata taggatgatt tgtatcggca ctcattccac
     4321 catgcattcc gattaacccc gcatagccag ttgaactttg ctactctgtg agaggtagta
     4381 ccttctatcc agtgcggatt taattaatgg aataaatgat tatgagtgaa aatgatacaa
     4441 ccccaaagaa gtctacaagc cagattaaca aagcggtatt ctttacatct gctttgctaa
     4501 ttttccttct tgtcgccttt gccgccgtat tcccggatgt cgccgacaaa aattttaaac
     4561 tacttcagca acaaatcttc acgaatgcca gctggttcta catacttgct gtggccctga
     4621 ttttactgag cgtcacgttc cttggactct cacgttacgg tgatataaag ctgggcccag
     4681 accatgcgca gcctgatttc agctaccact cctggttcgc gatgcttttt tcggcaggga
     4741 tggggatcgg cctgatgttc tttggcgttg ccgaacctgt gatgcattat ctttcgccac
     4801 ctgtcggcac tccagagacc gttgcggcag cgaaggaagc catgcgcctg acgttcttcc
     4861 actggggtct gcacgcatgg gcaatttatg ccatcgtggc gctgattctg gcctttttca
     4921 gttaccgtca cggtctgcct ttaacgctgc gttctgcgct ctatcccatt atcggcgatc
     4981 gcatttacgg gcctataggc cacgcggttg atatcttcgc cgtgataggc actgtctttg
     5041 gcgttgcaac atcgctgggt tacggtgtat tgcaggtgaa tgccggtttg aaccatcttt
     5101 tcggcgtgcc catcaatgaa acggtacagg tcattttgat cgtggtcatc acggggttag
     5161 cgacgatttc ggtggtgtcc ggcctggaca agggaatacg catcctgtct gagcttaatc
     5221 tgggcctggc ggtgttgctc ctggcgctgg tcttgtgcct gggtcccact gtgcttctgc
     5281 tgaagtcatt tgtggaaaat acgggtggtt atctgtcgga actggtgagt aaaacgttca
     5341 acctttacgc gtatgaaccc aagtcgagca actggctggg gggctggaca ttattatact
     5401 ggggatggtg gctttcatgg tcgccgtttg taggtatgtt cattgcacgc gtttcccgcg
     5461 gcaggacaat tcgtgagttc gtgactggcg tgttgtttgt tcccgccggc tttacgctca
     5521 tgtggatgac ggtgtttgga aacagcgcca tctatctcat catgaaccag ggcgccactg
     5581 acctcgccaa taccgttcag caggatgttg cgcttgccct gtttaatttc ctggagcatt
     5641 tcccgttctc ttccgtgctg tcattcattg caatggcgat ggtcatcgtc ttctttgtaa
     5701 cgtctgctga ttcgggggca atggttgtgg atactctggc atcaggtggg gtggcaaaca
     5761 cgcccgtctg gcagcgaatt ttctgggcat cgctcatggg cgttgttgct attgccctgc
     5821 tgatcgcagg ggggctgagt gcgttgcaaa ccgtaacgat agcgagtgca ttgcccttct
     5881 cagttatctt attaatatcc atatacggac tgttaaaagc cctgcgtcgg gatttgacca
     5941 agcgtgaaag ccttagcatg gcgactattg ctcctacggc tgcacgtaac ccaattccct
     6001 ggcagagaag gttacgcaat atcgcgtatt tgccgaagcg ctctcttgtg aaacgtttta
     6061 tggacgacat tatccaaccc gccatgacgc tggttcagga ggaacttaac aagcagggaa
     6121 cgataagcca catcagtgac gccgccgagg atcgtattcg ccttgaagtc gatttgggca
     6181 acgagctgaa ttatatatat gaagtgaggc ttcgcgggta taactcaccg accttcgcgc
     6241 tcgctgcgct ggataatgat gagcagcaga gtgaacaaca tcgatattat cgcgctgaag
     6301 tctatctcaa agagggcggc caaaattatg atgtaatggg ctggaatcag gaacagctga
     6361 ttaatgacat actggaccag tacgaaaaac acctgcactt cctgcatctg gttcgttaac
     6421 agcaacatgc cgccacagag gcggcattta tttatcccgg ctgcaatatg agggaatgca
     6481 gaatgatttc acgctggaaa tggatgctga agcagacatt taaaaaacta tggttcaggg
     6541 caacgttatt cgcaattgtc gcaataataa cggccctttt atcaattctt tttaaatcaa
     6601 tgatacctga gtcggtttcc gtgaaggttg gtgcggaagc agtcgataac attctgaaca
     6661 tactggcatc gagtatgctg gcagtgacca cattttcgct gagcatcatg gtcacagcct
     6721 acggttcagc cactactaat gtgactccca gagctactcg tttagttgtt gaagacgtca
     6781 ccacacaaaa tgtactggcc accttcatcg gttcttttct cttcagtctg gtagggatta
     6841 ttgccctcaa tatgggagct tatggagaaa gggggagagt cattttattc attgtaacac
     6901 tggttgtcat tgccttaatc ctcatcacat tgcttcgctg gatacagcat ttgacctctc
     6961 tggggagggt tggtgagaca acggcaaaag tagaacaggc ggccatcgaa acatttattg
     7021 cgagagcaag aaatccctgt ctcggcggat atccatggct tgagaacaat gaacagccga
     7081 aaggaacggt tgcagtttat ccgaaaaaga ttggctatgt tgaatatgtt gatatggtga
     7141 aactcagcaa gctgctgacc aatgatcccc gtcatgtata cctcgtggcg cagccgggca
     7201 gtttcataca tccgtccacg ccagttttgt acctgagtca gggccaggag tcatcaatca
     7261 gcaacgattt acttgagacg attattgtct cggatgtacg ttcatttgct caggaccccc
     7321 gattttgtct tagcgtcatg gccgaaatag cctgccgagc cctttccccc gcagtgaacg
     7381 atcctggaac cgccattgat gtcattggca gaggtgtacg tatactttcc gcttacgcgc
     7441 agaataaatc tgatgaaata gaagtgaaat atccttctgt acatgttgca ccacttcaga
     7501 ataacgatct acttgaagac tttttctcac ctgttgcgcg cgatggtgcc agtatgaggg
     7561 aaattcagat aagggtactt aaaggactgt cgatgctgag taagggttgg cctggaattt
     7621 ttgctgaagc cgcacaaacc ctagcatttg aaacattaga gcatgctact cgtgctgacc
     7681 atatagattc tgacagatat ctaataaaat caatttatta taatttattt agtggcgaag
     7741 attctaataa aaaaccatag ttgcggagca agcgatacgg ttgttggcaa gtcaagggca
     7801 aagcattcgc gtgagagtgg gcgtctgctt tgtgccagaa gcggacatac gtaacttatg
     7861 atacgcatcg ccaatcatcc gtttcaaaca tcaacgcgga aacgttttaa gaatgtaatc
     7921 ccgaacatag gcggttcggc tgttattagc cgtccttttg ttccagacca gataaagatg
     7981 attagtcgga gcgaggtctg caggtgtcac tgaaatcagg cgaccacttt tcaggtcatt
     8041 gtcacagtga taatccggca gcactgtcca cccgtggccg tcacagacca agtcgcggat
     8101 aatacgtaaa tccggaatgg tgaatgtagc ctgcatctca ggggctctct gaaacatgga
     8161 ggcccagagc gctctaatca gtggcaactc ttcgtcataa gcaatcagcg gtaatgaggc
     8221 aagcgtttca gcaccaggtt ctgatccgat gatctctgac atggcgggag catgaacaag
     8281 gagcatgcgt tcagtcagta gatgtgcata gccgtacaga cggtcatcag gcatagatgc
     8341 ggtaacagcg agatcggcgc tcccgtcatg caaaagggaa taaatacgtt gtttattgcc
     8401 ggtatgaaag cgtaggcgat acccctcctt cattagtgga gccattactg tcccaagccg
     8461 ggaatgaata aaatctggcg gtcctgccag attaattgtt cccccgcgta cagagcctga
     8521 ccggaaagag gacagctttt cttccagacc atcaatgaag ggtgaaactg agcgggcaag
     8581 ctcatccgca gcttcggttg ccgtcacgcc tctggcctgc ctgataaaca gacgcttacc
     8641 aaccagagtc tcaagagact ggatatgctg tgacgccgcg ggctgagtga tgcccagtag
     8701 ttctgccgcc tttgaaaagg agccagagcg ataggcctca agaaacgttc gcagatggct
     8761 cagatggctc atagtgaagc gcctcgctga tgaagtttat cgttacaggg agcctcatgc
     8821 agagcaaggg ccagttcact cacgtaatca tcccgtattc ccaacgcacc cagagtggcc
     8881 tgcagtttcc atacataatc tgcattcgtg ccaatgtctc cacgggcaga tgcgatgctc
     8941 aacgctacgg tcgctgtaag attgtcctgt tcataatgcg gatgcagtct gtctgatacg
     9001 aaagccagcg cctgaatttc ctcaccgttt cctagcgaaa cgtctgccca gaccggacgg
     9061 taaagaccgg ttatcatctc gcgcgtccag acaatttcca gctcattaat aagagtcgat
     9121 tccggcagtc tgaacgcaat accttcggta attccacctt cgtcgagcga taacatcctg
     9181 ccggggacat caggagtcgc tcgtccggat atcaaacgaa tgcagaaact gcgctgccat
     9241 cctttcagac gggcccgttg catttcttca atcgccagca cgggattcca catcaatgac
     9301 ccgtaggcaa atatccagac aggagcaccc gtcgggcgct cctccagagt agagttcatt
     9361 gatgcgcgta ttttttcagc gtcccagagc atattcgccg ggagcagcgc ttcatgcacc
     9421 ctgacaaatg tgccattaag catattttca cgggtaagca tataacctcc ctttactggc
     9481 ctacgatttt aacctgttga aatttacgac tgtggctgcg acggatttgt gcaagcagca
     9541 acagcaggcc gagcagagaa acaatcgcac cgctgatacc ggtatacacg ataccggcgt
     9601 ggcttatagt aatgctgccc agtactgatc ccagcgctat acctgagttg aagcccgcaa
     9661 tattcaggcc ggcagcaaca ccatgcgcat gaggagcatg ttgctcagcg acaccaatca
     9721 gacgggcctg cagtgccggc actgcggcaa atgtcaccat acccagcagc gcaaccagaa
     9781 tgcccatgct caccggtgag gatgacagca gccacatacc aagcacgaca acaacaatgc
     9841 cgctaatgat gagtatattg gcacgattaa cccccatgct gtcggtaagt ttcccaccaa
     9901 gaatattacc taccgcagct gtaataccgt aaaccagcat aaacagactc accgttgctc
     9961 cggtaacccc tgtcacctgc gtcagaatag gtgcgataaa ggtgtaagcc gtaaacgagc
    10021 ctgcatacgc cagcaccgta accagcgcgc cggccagcaa cttacggtta aacagcatgc
    10081 cgatattctg cattgctgag tcactatgtt caccgggcac gctgcgtacg ggatctttca
    10141 taccgaacaa cagtccaagg aatccgaacg cgccaaaagc ggcaatagcg ccgagaactg
    10201 gacgccatga gaccaccccc cccaaccatg tactcagcgg tacgccgatt gccatcgcca
    10261 gagtaaagcc accaaatacc acagccaccg ctctgcctgc tttacccggt ccagcaagac
    10321 gagctgcggt actggatgcc gcagccagaa acaggccgtg tcccagacca gcaatgaacc
    10381 gcgctaacag gagctgaggt aatgtggctg caaacgcggc cgccagacta ccggcagtaa
    10441 acacaacggc agttgtaagc attacatttt tccggctcca gcttgctgtc agcgcagaca
    10501 gtacgggtgc cgcgaacgtt gcccccagtg cataggttgt aaccgctgcc ccggtctgct
    10561 ccacacccac acacaacccc tgagccataa cgtctgtcag cccaatgggg acaaattccg
    10621 cgagtccgag cgcaaaggca ctgatagtaa acacataaat aacaaatggc atgactggct
    10681 cctgatttaa gtaaggagtg cagtctgatt tagaaaaaca gataaataaa ataatgtaaa
    10741 cttcaggcgg gataaaatta tttatatcta aggcaaagat tcaatattcc caca
//
LOCUS       DAOFQD010000161        10552 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.161, whole genome shotgun sequence.
ACCESSION   DAOFQD010000161 DAOFQD010000000
VERSION     DAOFQD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.161"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..282
                     /gene="eutS"
                     /locus_tag="QBF46_004207"
     CDS             <1..282
                     /gene="eutS"
                     /locus_tag="QBF46_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDQ6676320.1"
                     /translation="AHLIAHPGEELAKKIGVPDAGAIGIMTLTPGETAMIAGDLALKA
                     ADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLNYTLCEMTKS"
     gene            295..774
                     /gene="eutP"
                     /locus_tag="QBF46_004208"
     CDS             295..774
                     /gene="eutP"
                     /locus_tag="QBF46_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDQ6676321.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            749..1449
                     /gene="eutQ"
                     /locus_tag="QBF46_004209"
                     /pseudo
     CDS             749..1449
                     /gene="eutQ"
                     /locus_tag="QBF46_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
     gene            1446..2249
                     /gene="eutT"
                     /locus_tag="QBF46_004210"
     CDS             1446..2249
                     /gene="eutT"
                     /locus_tag="QBF46_004210"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDQ6676322.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGD
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            2246..3262
                     /gene="pta"
                     /locus_tag="QBF46_004211"
     CDS             2246..3262
                     /gene="pta"
                     /locus_tag="QBF46_004211"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDQ6676323.1"
                     /translation="MIIERCRELALRVPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3301..3594
                     /gene="eutM"
                     /locus_tag="QBF46_004212"
                     /pseudo
     CDS             3301..3594
                     /gene="eutM"
                     /locus_tag="QBF46_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
     gene            3701..3988
                     /gene="eutN"
                     /locus_tag="QBF46_004213"
     CDS             3701..3988
                     /gene="eutN"
                     /locus_tag="QBF46_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDQ6676324.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4000..5403
                     /locus_tag="QBF46_004214"
     CDS             4000..5403
                     /locus_tag="QBF46_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDQ6676325.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5414..6250
                     /gene="eutJ"
                     /locus_tag="QBF46_004215"
     CDS             5414..6250
                     /gene="eutJ"
                     /locus_tag="QBF46_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDQ6676326.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6240..7427
                     /gene="eutG"
                     /locus_tag="QBF46_004216"
     CDS             6240..7427
                     /gene="eutG"
                     /locus_tag="QBF46_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDQ6676327.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7543..8769
                     /gene="eutH"
                     /locus_tag="QBF46_004217"
     CDS             7543..8769
                     /gene="eutH"
                     /locus_tag="QBF46_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDQ6676328.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            8766..10169
                     /gene="eutA"
                     /locus_tag="QBF46_004218"
     CDS             8766..10169
                     /gene="eutA"
                     /locus_tag="QBF46_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDQ6676329.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
BASE COUNT         2326 a         2754 c         3179 g         2293 t
ORIGIN      
        1 gcgcatctca tcgcgcatcc cggcgaggaa ctggcgaaga aaatcggcgt tccggatgct
       61 ggcgcaatcg gcattatgac gctaactcct ggcgaaacgg cgatgatcgc cggtgattta
      121 gcattgaaag ccgccgatgt gcatatcggt tttctcgacc gttttagcgg cgcgctggtg
      181 atttatggct cggtgggtgc agtagaagaa gcgttatcac aaaccgtcag tggtttaggt
      241 cgcttattaa attatacgct ttgtgaaatg accaaaagtt aatacgaggc attcatgaaa
      301 cgaattgcgt ttgtgggtac cgtcggggca gggaaaacaa cgttatttaa tgcgttgcag
      361 gggaattata ccctcgccag aaaaacacag gccgtggaat ttaatgataa gggcgatatt
      421 gatacgccgg gtgaatattt tagccatccc cgctggtatc acgccttaat taccacgcta
      481 caggatgtgg acatgctgat ctatgtccac ggcgcgaatg acccggaaag tcggttgcct
      541 gccgggttgc tggatattgg cgtcagcaaa cggcaaatcg ccgttatcag taaaacggac
      601 atgccggatg ctgacgtcgc cgcaacgcga aagttattgc ttgagactgg cttcgaagag
      661 ccgattttcg agctaaacag ccacgacccg caaagcgtgc agcaactggt ggattatctg
      721 gcatctctca ccaaacagga ggaagcaggt gaaaaaactc atcacagcga atgatattcg
      781 tgaagcacac gcgcgcggcg aacaggcaat gtccgtggtt ctgcgcgcca gcattattac
      841 cccggaagcc cgtgaagtgg cggatttact gggttttacc atcaccgaat gtgatgaatc
      901 gatcccggta acggcgtccg tgcccgccag cgtgccagcc gataaaaccg aaagccagcg
      961 catccgcgaa accatcattg cccagctgcc ggaaggccag tttaccgaaa gcctggtcgc
     1021 gcagctgatg gaaaaagtga tgaaggaaaa cagtcgctgg agcagggcgc aatgctgccg
     1081 agcttcaaat cggtgaccgg caaaggcggc atcaaagtga ttgatggcag cagcgtcaag
     1141 tttggtcgtt ttgatggcgc acaaccgcat tgcgtgggct tgactgatct tgtgaccgga
     1201 gacgacggca gcagcatggc cgccggtttt atgcagtggg agaacgcatt cttcccgtgg
     1261 accctgaact acgacgaaat cgacatggtg ctggaagggg agctgcatgt tcgccacgaa
     1321 ggccaaacca tgatcgccaa agcaggcgac gtgatgttta tcccgaaagg ctccagcatc
     1381 gaatttggta cgacatccag cgtgaaattc ctgtatgtcg cctggccggc taactggcaa
     1441 tccctatgaa agatttcatc accgaagcat ggctaagagc gaaccatacg ctcagcgaag
     1501 gcgcagagat ccatctccct gcggacagtc gcctgacgcc ctctgcccgg gagttgctgg
     1561 aaagccgcca tctgcgcatc aagtttattg acgagcaggg ccgcctgttt gttgacgatg
     1621 aacagcagca gccgcagccc gttcatgggc tgaccagtag cgatgaacat ccgcaggcgt
     1681 gctgcgaact gtgccgccag ccggtggcaa aaaagccgga tacgctgact cacctgtcgg
     1741 cggaaaaaat ggtcgccaaa agcgatccgc gtctgggttt tcgcgcggtt ctcgacagca
     1801 ccattgcgct ggcagtgtgg ctgcaaattg aactggcgga accgtggcag ccgtggctgg
     1861 cggatatccg ttcgcgtctt ggcaacatta tgcgcgccga tgcgctgggg gaaccgctgg
     1921 gcgatcaagc gattgtcggg ctttctgacg aagatctgca tcggctttct catcagccgc
     1981 tgcgctatct cgaccacgat catctggtgc cggaagccag ccacgggcgc gatgctgcgt
     2041 tgctcaatct gctacgcacc aaagtgcggg aaaccgaaac cgtagcggcg caggtcttta
     2101 tcacccgcag ctttgaagta ttacgcccgg acattttgca ggcgctgaac cgcctttcca
     2161 gcacggtcta cgtgatgatg atcctgagcg taacgaagcc gccgctaacg gtgaagcaga
     2221 tccaacaacg actgggggaa acgcaatgat tattgaacgt tgtcgtgaac tggcgttgcg
     2281 agtgcccgcc agagtggttt ttccggatgc gttagatcaa cgtgtgctga aagctgcgca
     2341 atatttacat caacaaggtc tggcaacgcc cattctggtc gccaatccgt ttgaacttcg
     2401 tcagtttgcg ctcagtcacg gcgtggcgat ggacgggcta caggtgattg atccgcatgg
     2461 caacctcgca atgcgggaag aatttgctca tcgctggctg gcccgcgcgg gcgaaaaaac
     2521 gccgccggat gcgctggaaa aacttaccga cccgctgatg ttcgccgcag caatggtcag
     2581 cgccggtaaa gcggatgtct gtatcgcggg caacctctct tccacggcga atgtgctgcg
     2641 tgccggatta cgcattatcg gcttgcagcc aggctgtaaa acgctctctt ccattttcct
     2701 gatgctgcca cagtacagtg gccccgcgtt gggctttgcc gattgcagcg tggtaccaca
     2761 gccgacggcg gcgcagctgg cggatatcgc gcttgccagc gccgaaacct ggcgcgccat
     2821 caccggagaa gagccgcgcg tggcgatgct gtcgttttcc agtaacggta gcgcccgtca
     2881 cccttgcgtt gccaatgtcc agcaggcgac agaaatcgtc cgtgagcgcg caccaaagct
     2941 ggtagtcgat ggggagttgc agtttgacgc cgccttcgtg ccggaagtgg cggcgcaaaa
     3001 agcgcctgcc agcccgctac agggcaaggc caatgtgatg gtttttccgt cgctggaagc
     3061 cggaaatatt ggctacaaaa tcgcacaacg actcggcgga tatcgtgccg tcgggccatt
     3121 gatacaagga cttgccgcgc cgatgcacga tctctctcgt ggttgtagcg tgcaggaaat
     3181 tatcgagctg gcgctggtgg cagctgtgcc gcgtcagaca gaagtaaacc gtgaaagcag
     3241 tttacaaaca ctggttgaat gaacggtccc gttctggacc ctgataaaga ggaaagcaca
     3301 atggaagcat aaggaatgat cgaaacccgg ggcctggttg cactgattga ggcttctgac
     3361 gcgatggtga aagctgcgcg cgtcaagttg gttggtgtta agcagattgg cggcggcctg
     3421 tgtactgcca tggttcgtgg tgatgttgca gcctgtaaag ccgcgaccga tgctggtgcc
     3481 gcagcggcac agcgtatcgg cgagctggta tctgtacacg ttatcccgcg tccgcacggc
     3541 gacctggaag aagtgttccc gatcggcctg aaaggcgata gcagcaacct gtaatcaccc
     3601 tgtgtgacgt cgattcgggc gtcacgctct ctcctttttc attttacctt ctgcggaggg
     3661 tagggggagc ttttacctga aatacgccac ggaggcgggt atgaaactgg cagtcgtcac
     3721 tggacaaatt gtttgtaccg tacgccatca cggactggcg catgacaaat tgctgatggt
     3781 ggaaatgatt gatccacatg gtaatcctga cgggcaatgc gccgtcgcta tcgacaatat
     3841 tggcgcggga accggggagt gggtgttact ggtgagtggc agttccgccc gccaggcgca
     3901 taaaagcgaa acgtcaccgg tcgatctgtg cgtgattggc attgtcgatg aggtggtgtc
     3961 tggcggtcag gtaattttcc acaaataagg cagaacatca tgaatcaaca ggatattgaa
     4021 caggtggtga aagcggtact gctgaaaatg caaagcagtg acacgccgtc cgccgccgtt
     4081 catgagatgg gcgttttcgc gtccctggat gacgccgttg cggcagccaa agtcgcccag
     4141 caagggttaa aaagcgtggc aatgcgccag ttagccattg ctgccattcg tgaagcaggc
     4201 gaaaaacacg ccagagattt agcggaactt gccgtcagtg aaaccggcat ggggcgcgtt
     4261 gaagataaat ttgcaaaaaa cgtcgctcag gcgcgcggca caccaggcgt tgagtgcctc
     4321 tctccgcaag tgctgacggg cgacaacggc ctgaccctaa ttgaaaacgc accctggggc
     4381 gtggtggctt cggtgacgcc ttccactaac ccggcggcaa ccgtaattaa caacgccatc
     4441 agcctgattg ccgcgggcaa cagcgtcatt tttgccccgc atccggcggc gaaaaaagtc
     4501 tcccagcggg cgattacgct gctcaaccag gcgattgttg ccgcaggtgg gccggaaaac
     4561 ttactggtta ctgtggcaaa tccggatatc gaaaccgcgc aacgcttgtt caagtttccg
     4621 ggtatcggcc tgctggtggt aaccggcggc gaagcggtag tagaagcggc gcgtaaacac
     4681 accaataaac gtctgattgc cgcaggcgct ggcaacccgc cggtagtggt ggatgaaacc
     4741 gctgacctcg cccgtgctgc tcagtccatc gtcaaaggcg cgtctttcga taacaacatc
     4801 atttgtgccg acgaaaaggt actgattgtt gttgatagcg tagccgatga actgatgcgt
     4861 ctgatggaag gccagcacgc ggtgaaactg accgcagaac aggcgcagca gctgcaaccg
     4921 gtgttgctga aaaatatcga cgagcgcgga aaaggcaccg tcagccgtga ctgggttggt
     4981 cgcgatgcgg cgaaaatcgc ggcggcaatc ggcctgaacg tgccgcaaga aacgcgtttg
     5041 ctgtttgtgg aaaccactgc agaacatccg tttgccgtga ccgaactgat gatgccggtg
     5101 ctgcccgtcg tgcgcgtcgc caacgtggcg gatgccattg cgctggcggt gaaactggaa
     5161 ggcggttgcc accacacggc ggcaatgcac tcgcgcaaca tcgaaaacat gaaccagatg
     5221 gcgaatgcta ttgataccag cattttcgtt aagaacggac cgtgcattgc cgggctgggg
     5281 ctgggcgggg aaggctggac caccatgacc atcaccacgc caaccggtga aggggtaacc
     5341 agcgcgcgta cgtttgtccg tctgcgtcgc tgtgtattag tcgatgcgtt tcgcattgtt
     5401 taaggagtaa cagatggcgc acgacgaaca atggctcacc ccacgtctgc aaacggcggc
     5461 aacgctgtgt aaccagacgc ctgccgcgac ggaatctccg ctgtggctgg gggtggatct
     5521 gggcacctgc gatgtggtgt cgatggttgt cgaccgcgac ggtcagccgg tggcggtatg
     5581 cctcgactgg gccgatgtgg tccgcgacgg catcgtctgg gatttcttcg gcgctgtcac
     5641 cattgttcgt cgccatctcg acacgctcga gcagcaattc ggtcgccgtt ttagccatgc
     5701 ggcgacttca tttcctccag gtacggaccc gcgtatttcc atcaacgtgc tggagtctgc
     5761 tggactggaa gtgagccatg tgctggatga gccaaccgca gttgcggatc tgctgcaact
     5821 ggacaacgcc ggtgtagtgg atatcggcgg cggcactacc ggcattgcca tcgtgaaaaa
     5881 gggcaaagtg acgtactcgg cggatgaagc caccggcgga catcacatct ctctgactct
     5941 cgccggaaat cgccgtattt cgctggaaga ggcggagcag tacaagcgcg gtcacggtga
     6001 agagatttgg cctgcggtga aaccggtgta cgaaaaaatg gcggacattg tcgcccggca
     6061 tattgaaggg cagggcatta ctgatttatg gctggcgggc ggctcctgta tgcaaccggg
     6121 cgtggcagaa ctgtttcgca aacaattccc ggcgttacag gtgcatttac cgcagcacag
     6181 tttgtttatg accccgctgg cgatcgccag tagtgggcga gagaaagcgg aggggctata
     6241 tgcaaaatga attgcagacc gcgctctttc aggcgttcga taccctgaat ctgcaacggg
     6301 taaaaacatt tagcgttcca ccggtgacgc tttgcggtcc gggctcggtg agcagttgcg
     6361 gacagcaagc gcaaacgcgt gggctgaaac atctgttcgt gatggcagac agctttttgc
     6421 atcaggcagg gatgaccgcc gggctgacgc gtagcctggc cgttaaaggt atcgccatga
     6481 cgctctggcc atgtccggtg ggcgaaccgt gcattaccga cgtgtgtgca gccgtggcgc
     6541 agttgcgtga gtcaggttgt gacggggtga tcgcatttgg cggcggctcg gtgctggatg
     6601 cagcaaaagc cgtggcgttg ctggtgacga accccgatag cacgctggcg gaaatgtcag
     6661 aaaccagcgt tctgcaaccg cgcctgccgt tgattgccat tccaacgacc gccggaaccg
     6721 gctctgaaac caccaacgta acggtgatta tcgacgcggt gagcgggcgc aagcaggtgt
     6781 tagcccatgc ttcgctgatg ccggatgtgg cgattctcga cgccgcgttg accgaaggtg
     6841 tgccgtcgca tgtcacggcg atgaccggca ttgatgcgtt aacccatgcc attgaagcat
     6901 acagcgccct gaacgctaca ccgtttaccg acagtctggc gattggtgcc attgcgatga
     6961 ttggcaaatc gctgccgaaa gcggtgggct acggtcacga ccttgccgcg cgcgagagca
     7021 tgttgctggc ttcatgtatg gcgggaatgg cgttttccag tgcgggtctt gggttgtgcc
     7081 acgcgatggc gcatcagccg ggcgcggcgc tgcatattcc acacggtctg gcgaacgcca
     7141 tgttgctgcc aacggtgatg gaatttaacc ggatggtttg tcgtgaacgc tttagtcaga
     7201 ttggtcgggc actgcgaact aaaaaatccg acgatcgtga cgctattaac gcggtaagtg
     7261 agctgattgc ggaagttggg attggtaaac gactgggcga tgttggtgcg acatctgcgc
     7321 attacggcgc atgggcgcag gccgcgctgg aagatatttg tctgcgcagt aacccgcgta
     7381 ccgccagcct ggagcagatt gtcggcctgt acgcagcggc gcaataaatg ccggatgcga
     7441 cgcttgccgc gtcttatccg gcctacggga ttgcacatgt agggcggata aggcttcacg
     7501 cagcatccgc taataaataa caactaacag ggagtaaagg cgatgggaat taacgaaatc
     7561 atcatgtaca tcatgatgtt ctttatgctg atagctgccg tagacaggat cctgtcgcag
     7621 ttcggcggtt ctgctcgttt cctcggtaag ttcggtaaaa gtatcgaagg atcaggcggt
     7681 cagttcgaag aaggctttat ggcaatgggc gctctgggtc tggcgatggt cggtatgacc
     7741 gcgctggcac cggtactggc tcacgtactc gggccggtaa ttatcccggt ttacgaaatg
     7801 ctcggcgcaa acccgtcaat gttcgccgga acgctgctgg cgtgcgatat gggcggcttc
     7861 ttcctcgcca aagagctggc gggcggcgac gtagcagcgt ggctatactc tgggttaatt
     7921 ctcgggtcga tgatggggcc aacgattgtg ttttccattc cggtggcgct cggcattatc
     7981 gaaccttctg accgtcgtta tctggcgctc ggcgtgctgg cgggcattgt gaccattccg
     8041 attggttgta tcgctggtgg tctggtagct atgtactccg gtgttcagat caacggccag
     8101 ccggtggaat tcactttcgc cctgatcctg atgaacatga tcccggtgat cattgttgcg
     8161 attctggtgg cgctggggct gaaattcatc ccggaaaaaa tgatcaacgg cttccagatc
     8221 ttcgccaaat tcctcgttgc attgatcacc ctcggtcttg ccgctgcggt agtgaaattc
     8281 ctccttggtt gggaactgat ccccggtctg gatcctatct ttatggcccc tggcgataaa
     8341 cccggtgagg tgatgcgcgc cattgaagtt atcggttcta tctcctgcgt tctgttaggg
     8401 gcgtatccga tggtgctgct gctgacccgc tggtttgaaa aaccgctgat gagcgtcggt
     8461 aaagtgctga atatgaacaa catcgcggca gccggcatgg tggcaacgct tgccaacaac
     8521 atcccgatgt tcggcatgat gaagcagatg gatacccgcg gcaaagtcat caactgcgcc
     8581 ttcgccgttt ccgctgcttt cgccctgggc gaccacttag gcttcgccgc tgccaacatg
     8641 aatgccatga tcttcccgat gattgtcggc aagttgatcg gcggcgtaac ggcgattggc
     8701 gtggcgatga tgctggtgcc aaaagacgac gcgaccgcgg ctaaaaccga agcggaggca
     8761 caatcgtgaa cactcgccag ctactgagcg tcggtatcga tatcggcacc accaccaccc
     8821 aggtgatttt ctcccacctg gaactggtta accgtgcggc ggtgtcgcag gtgccgcgct
     8881 acgaattcat caaacgcgaa attagctggc aaagcccggt gttctttacc cctgtcgata
     8941 aacagggtgg tttaaaagaa gcggaactga aatcgctgat tttagagcaa tatcaggctg
     9001 cgggcattgc gccggaaagc gttgattctg gtgccatcat catcaccggt gaaagcgcga
     9061 aaacccgcaa tgctcgcccg gcggtgatgg cgctctctca gtcgctgggc gattttgtcg
     9121 ttgccagcgc cgggccgcac ctcgaatccg tgatcgccgg tcacggagcc ggggcacaaa
     9181 ccctttctga acaacggctg tgtcgggtac tgaatatcga catcggcggt ggcaccgcga
     9241 actacgccct gttcgatgcc ggaaaaatca gcggcactgc ctgcctcaac gtcggtggtc
     9301 gcctgctgga aaccgacagc caggggcgcg tggtttacgc tcataaaccg gggcagatga
     9361 ttgtggatga gtgcttcggc gcaggcaccg acgcccgttc gctgaccggc gcgcagctgg
     9421 tgcaggttac ccggcggatg gcagagctga ttgtcgaagt gattgacgga acgctttcgc
     9481 cgctcgcgca ggcattaatg caaaccggtt tgctgcccgc aggtgttacg cccgaaatca
     9541 ttacgctttc tggcggtgtg ggcgaatgtt atcgccacca gcccgccgac ccgttctgtt
     9601 ttgccgatat tggcccgctg ctggcaacgg cgctgcatga ccatccgcgc ctgcgtgaga
     9661 tgaacgtgca gtttccggcg caaaccgtac gcgccacggt gattggcgcg ggtgcacata
     9721 ccctttcgct ctctggcagc acaatctggc tggagggcgt acaactgccg ctgcgcaatt
     9781 tgccggtggc gatcccgatt gatgaaacgg atctggtgag tgcctggcaa caggcgctgc
     9841 ttcagctgga tcttgatcct aaaactgacg cgtacgtgct ggcgcttccc gcctcgctgc
     9901 ctgtgcgtta cgccgcggta ctgacggtca tcaacgcgct ggtcgatttc gtcgcgcgtt
     9961 ttccgaatcc gcatcccctg ctggtggtgg ccgggcagga ctttggtaaa gctctgggca
    10021 tgttgttgcg cccacagcta caacaactcc cgttggcagt cattgacgaa gtgattgtcc
    10081 gcgcggggga ctatatcgac attggtacgc ctctttttgg cggatcggtt gtgccggtga
    10141 cggtgaaatc actcgcattt ccttcctgaa tacggtagtt atcaggtatg gtgcttggtg
    10201 gctgggtgtt agtgggatta tatccggcag tgtatccggt taaggtgatt ggtctggcaa
    10261 gatgattgga tgcttttgtg gtgaaaaatg ttttggtgtt ttagcataac cctgaagaaa
    10321 gaataaaaat aaaaaggaaa aaaccaaaac cagaaaagaa gagaaccgga aagagaagaa
    10381 aagagaaaat gaaaaataaa aaccaaaaaa gaaagaatac ttaaatcttc gcatgttctt
    10441 cgggtggtac gcaagtttgt tagtctgacc gcgcaataaa ttttcgctta gaaaataata
    10501 agttacataa tattctgctt ttttagataa atataaccag aaaaaatcat tg
//
LOCUS       DAOFQD010000162        10485 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.162, whole genome shotgun sequence.
ACCESSION   DAOFQD010000162 DAOFQD010000000
VERSION     DAOFQD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.162"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            77..1228
                     /gene="yiaY"
                     /locus_tag="QBF46_004219"
     CDS             77..1228
                     /gene="yiaY"
                     /locus_tag="QBF46_004219"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDQ6676330.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1393..2931
                     /gene="aldB"
                     /locus_tag="QBF46_004220"
     CDS             1393..2931
                     /gene="aldB"
                     /locus_tag="QBF46_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDQ6676331.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3150..3305)
                     /locus_tag="QBF46_004221"
     CDS             complement(3150..3305)
                     /locus_tag="QBF46_004221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676332.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            3476..3799
                     /locus_tag="QBF46_004222"
     CDS             3476..3799
                     /locus_tag="QBF46_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDQ6676333.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3805..4941
                     /locus_tag="QBF46_004223"
     CDS             3805..4941
                     /locus_tag="QBF46_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ6676334.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4938..5912)
                     /locus_tag="QBF46_004224"
     CDS             complement(4938..5912)
                     /locus_tag="QBF46_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6676335.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            6036..6776
                     /locus_tag="QBF46_004225"
     CDS             6036..6776
                     /locus_tag="QBF46_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDQ6676336.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(6906..8876)
                     /locus_tag="QBF46_004226"
     CDS             complement(6906..8876)
                     /locus_tag="QBF46_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDQ6676337.1"
                     /translation="MNISEVDLRKLTVSDPFLGQYQQLVRDVVISYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDRVFGPDEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFAYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGNGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(8887..10287)
                     /locus_tag="QBF46_004227"
     CDS             complement(8887..10287)
                     /locus_tag="QBF46_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6676338.1"
                     /translation="MTSTPITTADIANGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVVVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
BASE COUNT         2575 a         2577 c         2758 g         2575 t
ORIGIN      
        1 aataatactg atcatatttt cgaatgaaaa tcctcagtaa gctgcccgcc cttttttaca
       61 ctttcaggag tgtgttatgg catcttcaac tttctttatt ccttctgtga atgtcatcgg
      121 cgctgattca ttgactgatg caatgaatat gatggcagat tatggattta cccgtacctt
      181 aattgtcact gacaatatgt taacgaaatt aggtatggcg ggtgatgtgc aaaaagcact
      241 ggaagaacgc aatattttta gcgttattta tgatggcacc caacctaacc caaccacgga
      301 aaacgtcgcc gcaggtttga aattacttaa agaaaataat tgcgatagcg tgatttcctt
      361 aggcggtggt tctccgcatg actgtgcaaa aggtattgcg ctggtggcag ccaatggtgg
      421 tgatatccgt gattatgaag gcgttgaccg ctctgcaaaa ccgcagctgc cgatgatcgc
      481 catcaatacc actgcgggta cagcatcaga aatgactcgt ttctgcatca tcaccgacga
      541 agcgcgtcac atcaaaatgg cgattgttga taagcacgtg actccgctgc tttctgtcaa
      601 tgactcctcg ctgatgatcg gtatgccgaa gtcactgacc gccgccactg gtatggacgc
      661 cttaacgcac gctatcgaag cgtatgtttc tattgccgcc acgccgatca ctgacgcttg
      721 tgcactgaaa gccgtgacca tgattgccga aaacctgccg ttagccgttg aagatggcag
      781 taatgcgaaa gcgcgtgaag caatggctta tgcccagttc ctcgccggta tggcgttcaa
      841 taatgcttct ctgggttatg ttcatgcgat ggcgcaccag ctgggcggtt tctacaacct
      901 gccacacggt gtatgtaacg ccgttttgct gccgcatgtt caggtattca acagcaaagt
      961 cgccgccgca cgtctgcgtg actgtgccgc tgcaatgggc gtgaacgtga caggtaaaaa
     1021 cgatgcggaa ggtgctgaag cctgcattaa cgccatccgt gaactggcga agaaagtgga
     1081 tatcccggca ggcctacgcg acctgaacgt gaaagaagaa gatttcgcgg ttctggcgac
     1141 taatgccctg aaagatgcct gtggttttac taacccgatc caggcaactc acgaagaaat
     1201 tgtggcgatt tatcgcgcag cgatgtaatc atcatttcca caacggctgg caaattgtca
     1261 gccgcttttt caactatctc tgtaaccctt gcccgtaaat tcgtgatagc tgtcgtaaag
     1321 ctgttaccga ctggcgaaga tttcgccagt cacgtctacc cttgttatac ctcacaccgc
     1381 aaggagacga tcatgaccaa taatccccct tcagcacaga ttaagcccgg cgagtatggt
     1441 ttccccctca agttaaaagc ccgctatgac aactttattg gcggcgaatg ggtagcccct
     1501 gccgacggcg agtattacca gaatctgacg ccggtgaccg ggcagctgct gtgcgaagtg
     1561 gcgtcttcgg gcaaacgaga catcgatctg gcgctggatg ctgcgcacaa agtgaaagat
     1621 aaatgggcgc acacctcggt gcaggatcgt gcggcgattc tgtttaagat tgccgatcga
     1681 atggaacaaa acctcgagct gttagcgaca gctgaaacct gggataacgg caaacccatt
     1741 cgcgaaacca gtgctgctga tgtaccgctg gcgattgacc atttccgcta tttcgcctcg
     1801 tgtatccggg cacaggaagg cggtatcagt gaagttgata gcgaaaccgt ggcctatcat
     1861 ttccacgaac cgttaggcgt ggtggggcag attattccgt ggaacttccc gctgctgatg
     1921 gcgagctgga aaatggctcc cgcgctggcg gcgggcaact gtgtggtgct gaaacccgca
     1981 cgtcttaccc cgctttctgt actgctgcta atggaaatcg tcggtgattt actgccgccg
     2041 ggcgtggtga acgtggtcaa cggcgcaggt ggggaaattg gcgaatatct ggcgacctcg
     2101 aaacgcatcg ccaaagtggc gtttaccggc tcaacggaag tgggccaaca aattatgcaa
     2161 tacgccacgc aaaacattat tccggtgacg ctggagctgg gcggcaaatc gccaaatatc
     2221 ttctttgctg atgtgatgga tgaagaagat gcctttttcg ataaagcgct ggaaggcttt
     2281 gcactgtttg cctttaacca gggcgaagtt tgcacctgtc cgagtcgtgc tttagtgcag
     2341 gaatctatct acgaacgctt tatggaacgc gccatccgcc gtgtcgaaag cattcgtagc
     2401 ggtaacccgc tcgacagcgt gacgcaaatg ggcgcgcagg tttctcacgg gcaactggaa
     2461 accatcctca actacattga tatcggtaaa aaagagggcg ctgacgtgct cacaggcggg
     2521 cggcgcaagc tgctggaagg tgaactgaaa gacggctact acctcgaacc gacgattctg
     2581 tttggtcaga acaatatgcg ggtgttccag gaggagattt ttggcccggt gctggcggtg
     2641 accaccttca aaacgatgga agaagcgctg gagctggcga acgatacgca atatggcctg
     2701 ggcgcgggcg tctggagccg caacggtaat ctggcctata agatggggcg cggcatacag
     2761 gctgggcgcg tgtggaccaa ctgttatcac gcttacccgg cacatgcggc gtttggtggc
     2821 tacaaacaat caggtatcgg tcgcgaaacc cacaagatga tgctggagca ttaccagcaa
     2881 accaagtgcc tgctggtgag ctactcggat aaaccgttgg ggctgttctg atataagaag
     2941 ctggtcgcat tgggtattca ttgcctgatg cgacgcttac gcgtcttatc atgcctacgg
     3001 gaacctgacc gtaggccgga taaggcgtac acgccgcatc cggcataaac aaagcgcacg
     3061 ttgttacaat ttacgtttta aacctacgtg cgcaataacc cagactacgc caacagaagt
     3121 gcgtttctgg ggcgtgtttt gtcaggagtt taatctcgcg tttcgatggt gagcgggcga
     3181 accaggccgt aaatcaccat tggtgatccg aggccaatca gcaataaacg gtagataatt
     3241 gaaggtttaa tacgcttaat ctgtactgtg gatgttttac tggagttatt cattagtttg
     3301 gccaatagtt ccactatttc aatatgataa acctcgtcct gttgaaaaac cagacagcac
     3361 atttaagaaa gtatataaaa aacaatatgt cgttatataa ataaggaaat tgcactgcat
     3421 catggcattt cataatatgc gttaattgtt cttttttata ttctttgagg gaaatatgtt
     3481 cctggactat tttgcactgg gagtgcttat ttttgtattt ctggtgatct tctatgggat
     3541 cattatttta catgatattc cctacctgat tgccaaaaaa cgtaatcatc ctcatgccga
     3601 cgccattcat gttgctggtt gggtgagtct ttttacgtta catgttatct ggccgttttt
     3661 gtggatttgg gccacgcttt accgcccgga gcggggatgg ggaatgcaaa gccatgattc
     3721 atccgttatg caactgcaac agcgcattgc cgggctggag aaacagctcg ccgacatcaa
     3781 atcctcttct gccgagtaat acttatggat ttactgatta ttttgaccta tgtggctttt
     3841 gcatgggcaa tgtttaagat cttcaaaatt cccgtaaata aatggaccat tcccacagcg
     3901 gccctgggtg gaatatttat tgtcagcggt ctaattctgt taatgaacta taaccatccg
     3961 tataccttta aagcgcaaaa agcggttatt tctattcctg ttgtcccaca ggtgactggt
     4021 gtggtgattg aagtgacgga taagaaaaat acgctgatta aaaaaggtga ggtgctattt
     4081 cgactggacc cgacgcgtta tcaggcgcgg gtggatcggc tgatggcgga tatcgttacc
     4141 gcagaacata aacagcgggc gttgggcgca gagttagatg agatggcggc gaatactcag
     4201 caggcaaagg ccacgcggga taaattcgct aaagagtatc agcgttacgc acgtggcagc
     4261 caggcgaaag taaacccgtt ttcagaacgc gatatcgatg tggcgcggca aaattatctg
     4321 gcgcaggaag cctccgtgaa gtcatcggcg gcggaacaaa aacagatcca gagccagctg
     4381 gatagcctgg tgttgggtga acattctcaa atcgccagcc tgaaagcaca gctcgcggaa
     4441 gcaaaatata accttgagca gacgatagtg cgtgcgccga gcgatggtta tgtgacccag
     4501 gtactgattc gtccgggtac ctatgccgcg tcgctgccgc tacgtccggt gatggtgttt
     4561 atacccgatc agaaacgaca aatcgtggcg cagttccgtc agaactcctt gctgcgactg
     4621 gctcctggcg acgatgcgga agtggtgttt aatgctctgc ctggtaaggt attcagcggt
     4681 aagctggcag ccattagtcc agccgttccc ggcggagctt atcagtcgac cggcacctta
     4741 cagacgttaa acacagcgcc gggttcagat ggcgttatcg cgaccattga actggatgag
     4801 cacactgatt tgagcgcttt accagacggt atttacgccc aggtggcggt ttactctgat
     4861 catttcagcc atgtctcggt gatgcgcaaa gtgctattac gcatgaccag ctgggtgcat
     4921 tacctttatc tcgatcatta atcttcgctt tccgccgctt gttcaggccg cagttgctgc
     4981 gactgagcaa gctggtggcg aatttcacgc agcatttcat ccagcacata ctggacccgc
     5041 cagggctggt attcccgttt acggaagata gccaccagat ccagccacca ttcatagggt
     5101 tgctgaaagc agggcaccag cgtgccgtct tgcaggtcgc tctgcacact tttgtctggc
     5161 gcaaacacaa tccccaggtg atttcgtgcc agctccagcg cagattgcgt gttgtcgcaa
     5221 acgtaatttc ccttcacgcg ataatcccgc acctctttac tcccggcgac gttgaagcgc
     5281 cagatattcg cgtcgtcgat catcatcgag tcgatcaaaa tacaggagtg atgttcgagt
     5341 tcgtcaggac ggctaatggg atgtttctca agatagcgct ggctggcata agcggttacg
     5401 gcatattggg taataaaact ggcaaccagc gattcatctt ttggtggagc gtagctgatt
     5461 aaaacatcac aatcatcagg aaattcgacg ccttcataaa aggcattacg ctcaagattg
     5521 caggttttta gcgataaggt gatatcaccg atatctttaa ttttatcgat tacatgtttg
     5581 gataaatagg taattatgcc ggttggggcg tagatggtga cccgaccacg tttctcatgc
     5641 ttataatctg caataaaatt attaagctgc tcgtttctgt ccagcatgtc gttgatgtac
     5701 ggtaacagcg cggtaccaaa aggtgtgagc atcagctgcc gggtggttcg gtcaaagact
     5761 tttaaaccca cttttgattc aaaatcagca agatatttgc tgacgttggc ctgtgcgatg
     5821 ccaagtacag tcgcggcatg gctgatattt tcactggcag cgattaccga gataattttt
     5881 aactcccggt acttaagttg taattttgtc atcgcaacag cccaattata tataatttta
     5941 tatattacta tataaatatg aatcttgcac cggagaaatt gtaagcatta ctatgccgac
     6001 tcttttttat cttattttaa aatggaatac cgaacatgtt aattaaccgc aatattgtgg
     6061 cgttatttgc gttgcctttt atggcaagcg caactgcttc tgaattatcc attggtgccg
     6121 gtgcggctta taatgaatcg ccttatcgcg gttataatga aaatacaaag gcaattcccc
     6181 tgattagtta tgaaggtgat tctttttatg ttcgtcagac cacgttaggt tttattctgt
     6241 cgcaaagtga aaaaaatgaa cttagcctga ccgcatcctg gatgccgctg gaatttgacc
     6301 ctgccgataa tgacgattat gccatgcaac agcttgataa gcgtgatagt acggctatgg
     6361 cgggggttgc ctggtatcac cacgagcgtt ggggaactgt gaaagcctct gcggctgcgg
     6421 acgttctgga taacagcaac ggctgggtgg gggagctatc ggttttccac aaaatgcaga
     6481 taggtcgcct gtcgctgaca cctgcgctgg gcgtgctcta ttatgacgag aatttcagtg
     6541 actattacta tggcatttca gagagtgagt cccgtcgtag cggtctggca agttattccg
     6601 cgcaggatgc ctgggtgccc tatgtcagcc tgacggcaaa atacccgata ggagagcacg
     6661 tcgtattgat ggcgagtgca ggatacagcg agctgccgga agagattacc gacagcccga
     6721 tgattgatcg taatgagagt tttacctttg tcaccggcgt gagctggcgt ttttaattca
     6781 ccggtggatg tcggtgcggc tggcctacgc tctcgcaccg acttgtatgc ctgataagac
     6841 gtgttaagcg tcgcatcagg caatgtgctc cttacgttgg atgcggcgcg agtgccttat
     6901 ccaacctacg gatggcaatg cttttcctca ttcacccaga tccgcatttc gccttcaccc
     6961 cggttggccc agctaaacca cgggataaag gtcagagttt gcgtctggcg tttggctggc
     7021 gcgctgtcgt aatgccacag cggttgctgc tctggattgc tctgttcata ccggtaaccc
     7081 ggtgcctgga ttaagatctt atggcggaac aatccattac cttcaaatgt cgtaaatggc
     7141 gcatcggcgg gtagccacag attatgcagt gactcgccat tgtcggcctg ttccaggcaa
     7201 tacaccagcg ggccgcgctg aatcgccact tttccggcga cgtgacgcac cagcgggtta
     7261 ccgtaaacgc ggcgtaccgg catcggcaaa gtcagattca gcgtatcgcc ctcctgccat
     7321 tcgcgggtaa tgtgcaaata ccctttacga atatcctgct cgacctcttc cccattcaat
     7381 atgatctgcg gctgtgtgca ccagtccggc agacgtaaag ccagcgtatg acgtaccggc
     7441 tggggcgatt caaccgcaat cgtcacctgc tcctgccacg gatagttccc gctaacccgc
     7501 aggcgcagcg tgccattttc taccggcact tccatgctgt ttcctgcata tatgttgata
     7561 tacaacgcat cttcacgcgg cgtgtagaga taatgaccaa tcgaggtcag cacgcgggcg
     7621 atatttggcg gacaacaagc gcagccaaac caacgctggc ggatcggttt aacgtgatcg
     7681 tagatatggt tgaatttcag cgattttgga tgtacttcca gcggattcac atagaagaaa
     7741 tgtttgccat ccagcgccat gccgccgagc acggtgttgt acagcgcgcg ctccatcaca
     7801 tcggcatatt gactgtcgcc ttccatttcc agcattcgcc gggcgaacat catcaggccg
     7861 atggaagcac aactttcggc gtaaaccgtg tcattcggca gatcgtaatc gctagtgaac
     7921 gcttcgccgc tgctttgcga gccaatgccg ccggtaatat ataactgacg ctgggccata
     7981 ttgttccaca gcctcaggca gtcctgacgc ttgctgtcat cgtgacttaa acgcgcgaga
     8041 tgcgcgacgc cggtcatcag gtaggcaaaa cgtaccgcgt gaccgatggc ggtttgctgt
     8101 tgcgcaaggg gcaaatgtgc ttggctgtag gctttgtctt tcaccatcca cgccgggccg
     8161 taggtgtgcc agtgcgaggt ctgcccgcgc ttttcatatt cctggtcgta atagtgcggt
     8221 tgcgcaccac gctgttcgac aaaatagttc gtcagcgcca ggtagcgcgg ctcttcggtg
     8281 acttcgtaga gacgcatcag cgccagttca atttccggat gaccgggata accctgcagc
     8341 ttgtcttcat ctggaccaaa tacgcggtcg atatgatcgg ccagacggca aaccacctcc
     8401 agcaagcgtc gttttcccgt ggcctggaag aaggcgactc cggcttcaat cagatgacct
     8461 gcgcagtaaa gttcatgaca ctccgccaga ttgctccagc gttcttcggg tgcttttacc
     8521 gtaaagtaag tattgagata gccgtcttcg cattgggcgg aggcgatcag ttcgattacc
     8581 tcgtcggcgg ttttttccag ttcggcgtcc ggcttctggc acagcgacca ggctaccgct
     8641 tccagccatt tggcgacgtc gctgtcctga aacaccatcc cgtaaaattc accctcctga
     8701 agtccggcgg caatgcgaaa gttttcaatc gcatggctgg gttccgcttc tgggatacga
     8761 tcgttcaagg catcccactg ataagaaatc accacgtcgc ggaccagttg ttggtactga
     8821 ccgaggaacg gatcgctgac cgtcagttta cgcagatcga cttccgaaat gttcatcttg
     8881 tgctccttaa ggctgtacat gacgctgacg caggtcggta gctatgcgcg acatcatagg
     8941 attattgagt ttgcaaaagc ggatcgcccc tgccagcagc aggtggaata gcgcaggcag
     9001 caatgtttcc atcgccgtga tcccgccaag cgaactggcg gtctggtttt ccacgccagg
     9061 ctggtaggcg acaaaaataa acagcaggct gatgatcccg gcgctggagg cccaggccag
     9121 cttgatgaaa aacagattga aggcgaagtt catgccggaa gaacgtacgc gggttttcca
     9181 ctcgccgtag tcatcggcaa aggccatcaa tgagaagtgc aatggcaatg tgaagccaag
     9241 aatcacgcca ttaccgagga tcaccgccag ccacagcgtt tgataagccg ggccggaggg
     9301 caggaaccac atcaacaccg ccagtgcagc gaggagcagg ttggtgtagt aataaatttt
     9361 gacggtatcg aaacgccgcg ttaacgggct gacaaccacc gagccgataa tggaggcgaa
     9421 ggtgaccatg gtgaagaaca gcgacgtgta gcccgtactg ccttgtaaga cgtaggtaat
     9481 gaagtacata tacccaccgc cgcgaatatt aaagacgtta atcagcagga aagacatgac
     9541 cagcatcagc agcagttggt cgttgttccg cagcccggca agatgttcgc gcagggtaaa
     9601 tttccccatt gtggagagcg gcacccgttc acgaacccag aaaaaacagc acaggaacat
     9661 caccacggca atggcgcaca atacgccgac gcccagttga tagccgcgtg cagcgttgcc
     9721 ctgaccgaag agatctacca tccacggtaa gcctaccgaa accagaaatc ccgctacgcc
     9781 gcagagaacg aatcgccagg actggcagga gatcacttca ttgtggcggg tggtcatggt
     9841 gttgatcagc gcgcagtacg gtacgttgat ggcggtataa ccgactgaaa ggagcaggta
     9901 agtaccgaac gcccaggcga tttttactcc catgctggca tctggcacgg tgaacgtcag
     9961 gatgccgata atgccgatgg gaatggcaat ccatagctgc cacggacgaa agcgccccca
    10021 tcggctttgc gtccggtcgg caatgacgcc catcaccggg tcggagatgg catcaaacac
    10081 gcgcagtgca ataaacagcg tgccaaccag cgccggagtt aatccaaaaa cgtcggtgta
    10141 gaaaaaggtg agaaaattca tgatcaggca agttattacg gtgccgcctg cgtcacccag
    10201 gccgtagcct attttttccc gtacagataa cgcatcgtca attttaccgt tagcgatatc
    10261 agcggtagta atcggtgtag aggtcataga tattcctcgt gaatagggag caatttttag
    10321 ttttattgaa atgacctgat taattctgcc gtgaacggga atatcaacaa ggcgagaaaa
    10381 gagtaaaaaa aggtgacgat tccgacctga atttaaaagt gtgaaatgga tcaggtgaaa
    10441 ttaatgtttt tgattaataa tcaaaaggta atgtgtttaa tcatt
//
LOCUS       DAOFQD010000163         9817 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.163, whole genome shotgun sequence.
ACCESSION   DAOFQD010000163 DAOFQD010000000
VERSION     DAOFQD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.163"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            19..591
                     /locus_tag="QBF46_004228"
     CDS             19..591
                     /locus_tag="QBF46_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HDQ6676339.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            639..1370
                     /locus_tag="QBF46_004229"
     CDS             639..1370
                     /locus_tag="QBF46_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDQ6676340.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1395..3917
                     /locus_tag="QBF46_004230"
     CDS             1395..3917
                     /locus_tag="QBF46_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6676341.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKLVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3928..5001
                     /locus_tag="QBF46_004231"
     CDS             3928..5001
                     /locus_tag="QBF46_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6676342.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            5249..6289
                     /gene="pstS"
                     /locus_tag="QBF46_004232"
     CDS             5249..6289
                     /gene="pstS"
                     /locus_tag="QBF46_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDQ6676343.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6376..7335
                     /gene="pstC"
                     /locus_tag="QBF46_004233"
     CDS             6376..7335
                     /gene="pstC"
                     /locus_tag="QBF46_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDQ6676344.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7335..8225
                     /gene="pstA"
                     /locus_tag="QBF46_004234"
     CDS             7335..8225
                     /gene="pstA"
                     /locus_tag="QBF46_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDQ6676345.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8408..9181
                     /gene="pstB"
                     /locus_tag="QBF46_004235"
     CDS             8408..9181
                     /gene="pstB"
                     /locus_tag="QBF46_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDQ6676346.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9196..>9817
                     /gene="phoU"
                     /locus_tag="QBF46_004236"
     CDS             9196..>9817
                     /gene="phoU"
                     /locus_tag="QBF46_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDQ6676347.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQ"
BASE COUNT         2508 a         2273 c         2507 g         2529 t
ORIGIN      
        1 tatatcaatg gagtttttat gaagcgtaat attataggcg gtgcattcac tctggcatct
       61 ctaatgctgg ccgggcatgc actggcagaa gatggtgttg ttcacttcgt cggtgaaatt
      121 gtcgacacta cttgtgaagt tacctccgat acagccgatc aaattgtccc actgggtaaa
      181 gtcagtaaaa atgcattttc aggtgtaggt agtctggcgt cgccacagaa gttcagtatt
      241 aagcttgaaa attgcccggc aacgtacact caagcagccg ttcgttttga tggtacagaa
      301 gcgcctggcg gcgacggcga cctgaaagtg ggtacgccgc ttacagcagg caaccctggt
      361 gattttaccg gtacaggaca agcgattgcg gcaaccggcg ttggtattcg tatttttaac
      421 cagtccgata attcgcaggt taaactttat aacgactctg cttataccgc tatcgatgct
      481 gaaggcaagg ctgaaatgaa gtttattgca cgctatgtgg caaccaatgc gaccgtaacg
      541 gctggtacgg cgaacgcgga ttcacaattt actgtcgaat ataagaaata atttatcaag
      601 ggccttttta ttcagttggc tttggccagg gagtcgttat gtccagaaaa agttgtttaa
      661 tcaggctctt taaaacaaca gtttcatgta agttatttac cgcgattata ctcagcgttt
      721 ttttaggcca accagcatta acttatgcag gcgttgtcat tggtggtacg cgggtggttt
      781 acttaagtaa taatgctgat aaatctattt ctgtatttag caaagaagaa aaaatacctt
      841 atctcatcca ggcttgggta gatccattta ataaagagga taaaagcaaa gcgccgttta
      901 ccgttattcc gccagtatca cgattagaac cttcgcaaga aaaaatatta cgcattattc
      961 acaccaaagg tgtgtcattg cctgatgatc gcgagtcagt cttctggctg aatattaaaa
     1021 atattccgcc atcagcgagc aacaaagcga cgaactcgct ggaaatagca gtaaaaacgc
     1081 gtatcaagct gttctggcga ccagcaaata tccgccttat tcccgaagat gctgcaccga
     1141 aagtgaaatg gcgccgggaa gggcgaaatc tgatagcaga gaatcccaac ccaattcata
     1201 tcagtgtgat ggatgtcatc gttgatggcc atgatgtgcc cctcaatatg atccgtcctt
     1261 ttgaaacgct cacgttacct ttacctgcta attctgcggg aggccaaatg acctggcgat
     1321 ttattaatga ttacggcgca gtcagtgacc cgataaaaat gacgctgtaa gcaagggata
     1381 aggataggcg tattatgatg acgaccagaa tagtggttgg cctcacggca gggacgtgtc
     1441 tgattttctc gcaaaacctg atggccgagg tcagtgtatt caatccggcg cttctggaaa
     1501 tcgaccatca atccggagtc gatattcgcc agtttaatcg ggcaaacctg atgcccccag
     1561 gtgtttatag cgttgatatt tttatcaacg gtaaaatgtt tgaacgtcag gatgtgacat
     1621 ttgttcagga taatccagat gctgatctgc acgcttgctt tattgccatt aaaaaaacac
     1681 tgtcctcctt tggcataaaa gttgatgcgc tcaaatcgtt caatgatgtg gatgagacgg
     1741 tttgcctcga tcctgctcca cgtattgaag gctcatcctg gcagtttgac agtgataaat
     1801 tgcagctgaa tatatccatt ccccaaatct acatggacgc gatggcttat gattacatca
     1861 gccccacgcg ttgggatgag gggattaacg cgctcaccat caactacgat ttttctggtt
     1921 cacatacact acgttcagat tatggttcac aagagacaga taccagttat ctcaatctgc
     1981 gcaatggact gaatattgga ccgtggcggc tacgtaatta cagtacttta aacaccagcg
     2041 atggccgtgc ggaatacaac tccattagta cctggataca gcgcgatatt gccgcgttaa
     2101 gaagccagat tatgattggt gatacgtgga cggcgagcga tattttcgac agtacgcaaa
     2161 ttcgcggcgc gcgtttgtat actgataacg atatgctacc cgccagccag aatggctttg
     2221 ctcctgtggt tcgtgggatt gcaaagtcca acgccaccgt catcattcgg cagaatggct
     2281 acgtgattta tcagtctgcc gttccacaag gtgcttttga gatcaccgat ctcaacaccg
     2341 ccagtacagg tggcgatttg gacgtaacca tcaaagaaga agacggtagc gaacaacgat
     2401 tcacccaacc ttatgcttca ttggcgattc ttaaacgtga aggtcagaca gatgttgatg
     2461 tcagcgtggg tgaattgcgc gatgaagacg gatttacacc ggacgtcctt caggcgcaaa
     2521 tacttcatgg tttttcccac gggatcactt tatatggagg tatgcaggct gctgaaaatt
     2581 atggttctgc agctctgggt gtcggtaaag atcttggcgc tttgggcgca atttctttcg
     2641 atgtgacaca tgctcgtgcg aattttagcc atgatgatac agaaacgggt cagtcatatc
     2701 gctttctcta ttcaaaacga tttgacgaca cagacactag cttgcgcctg gttggctatc
     2761 gttactccac cgagggctac tataccctca atgagtgggc atcgcggcgc aacagccctg
     2821 aagacttttg ggaaacaggt aaccgacgta gtcgcgtgga gggaacgcta acgcagtcgt
     2881 tggggagaga ttatggcaat ttatacctga cattaagccg gcaacaatac tggcataccg
     2941 atgatgtcga acgattaatg caatttggct acagcagtag ctggaagcgt ctctcgtgga
     3001 acgtctcctg gagttattcc aatactgcca gacaggggac ggggaacaac catgccagtg
     3061 ataacaccag tgagcagatc tacatgctct ctttatctgt tcctttatcg ggctggtggg
     3121 gtaatagtta cgccacctat tctgtttcgc aaaacgataa ttccggtagc tcacatcaac
     3181 tcggactcag cggtacggcg ctggaaagaa ataacctttc atggaattta atgcagtcct
     3241 ataacagtca tgatgatgag gttggcggta atatgtccct gacctatgat ggctcttatg
     3301 gcacggtgaa cggcagctat aactacagcc aaaattccca gaggctgaat tatggtatca
     3361 gagggggaat tctggcacac agcgaagggg taacgttaag tcaggagtta ggtgaaacta
     3421 ttgctcttgt taaagcacct ggggccgccg ggttagaaat agataatatg cgcggtgctg
     3481 cgacggactg gcgcggctat acggtcaaga cacagctaaa cccttatgat gaaaatcggg
     3541 tagcaatcag cgataactat ttctcgaagt cgaatataga acttgataat accgtcgtta
     3601 cgatggttcc cacgcgtggt gcagtggtta aagcggagtt tgtgactcat gtgggttatc
     3661 gcgttctctt cagagtgtta aatgcaaatg gtaaactggt accttttgga gccattgctg
     3721 cgatacaaga tgcaagtttg gcagattcag gaattgtcgg tgaccgtggc gaactttatc
     3781 tttctggtct accagaaaaa ggacaggtta cgttatcctg gggagaaaac gcctcaacaa
     3841 aatgcatctt caattattca ctttcgacac cagaaagtga gagcggatta attgaacagg
     3901 gtgtgacatg tcattaaaag gatttttatg aacaagtata tcaaacagtg gtgctttgct
     3961 gtgtttatgc tctcgttaag tagtgtagca cttgcagctc ctaaaggtat ctgtacccct
     4021 gataatggcg tatttcacag tacgcttgat ttctcgggat atcttattac cgcgaacgaa
     4081 aataaggttg gaacgacatt taacaccact gtaactaatg gatcctcata tcctggacgc
     4141 tgtcattgtg atactggtaa cgtaggagaa ttcccctata tctattatac gtcaaaaata
     4201 aaccaggcat taacttatgc tggggttcac tctaatatta attattatga tcttaatccg
     4261 aaccttgatg ttgggattgc tatagatatt cttggggttg ggtatgtgaa tgcacctttt
     4321 gaatatcatg ctaacaaccc gtctggtaat acaaagtaca attgcaaccg cattgaacct
     4381 ttaagtatat ctagtggggc taaagcgata gtatattttt atatcaagaa gacatttgca
     4441 ggaaaattga ttattcctga aacaaagata gtgacattgt atggaacaat tagccgtgac
     4501 accccggtgg attactcaca gccgatggct gatgtttata ttcggggcga tattacagct
     4561 ccgcaaagtt gcgaaataaa taatttacag ccagtttatt ttgattttaa agaaatacca
     4621 gctgcagatt tttcatctgt tgttggaagt gcggtaacaa cacataaaat tactaaaacg
     4681 gtcactattg agtgtgaaaa cctggggata ctgaataccg atgatatcag tacgtctttt
     4741 tatgctaccg aacccaatac ggacaattca atggtcgtaa cttcaaactc taacgtgggg
     4801 ataaaaattt acgacaaaaa taataaggaa atcaaagtga acggtggtga gttgccaaca
     4861 gacatgggta aatcaacagt gtatggtgaa aaatcaggta gtgtaacttt ttcagctgct
     4921 cctgcgagcc ttactggagc tcgcccagcc ccggggcaat ttactgcaac agcgacgata
     4981 actgttgaga ttgtgcgcta ataggtctga caacgattgt tcatcacatc gttgtcatct
     5041 cacccatttc tttatcactt ttaaatgcat catgacaaaa agatgacatg tgggattttc
     5101 atcttcacat ttaaaatcag taagttattt ggtaattcct ttctgtcata aaaatgtcat
     5161 attccttaca tataactgtc acctgtttgt cctattttgc ttctcgtagc caacaaacaa
     5221 tgctttatga atcctcccag gagacattat gaaagtaatg cgtaccaccg tcgcaactgt
     5281 tgtcgccgcg accttatcga tgagcgcttt ctctgtgttt gcagaagcaa gcctgacagg
     5341 tgcaggtgca accttccctg cgccggtgta tgccaaatgg gctgacactt accagaaaga
     5401 aaccggtaat aaagttaact accagggtat cggttcttcc ggtggcgtaa aacagattat
     5461 cgctaatacc gttgattttg gtgcctctga cgcgccgctg tctgacgaaa aactggctca
     5521 ggaaggtctg ttccagttcc cgaccgtgat tggcggcgtg gtgctggcgg ttaacattcc
     5581 agggctgaag tctggcgaac tggtgctgga tggtaaaacc ctcggcgaca tctacctggg
     5641 caaaatcaag aagtgggatg atgaagccat cgccaaactg aatccgggtc tgaaactgcc
     5701 ttcacaaaac attgctgtag tacgccgcgc agatggctcc gggacttcct tcgtcttcac
     5761 cagctacctg gcgaaagtga acgaagagtg gaaaaacaac gttggtactg gctctaccgt
     5821 aaaatggccg atcggtctgg gcggtaaagg taacgacggt atcgccgcgt tcgttcagcg
     5881 tctgccgggt gcaattggtt atgttgaata tgcttacgcg aagcagaaca acctggcgta
     5941 caccaaactg atctccgctg atggtaaacc ggttagtccg accgaagaaa acttcgctaa
     6001 tgcagcaaaa ggtgcagact ggagcaaaac cttcgctcag gatctgacca accagaaagg
     6061 cgaagatgca tggcctatta cctctaccac gttcattctg atccacaaag atcagaagaa
     6121 accagaacaa ggcacagaag tgctgaaatt cttcgactgg gcgtacaaaa ccggggctaa
     6181 acaggcgaac gacctggatt acgccagcct gccggatagt gtagttgaac aggttcgcgc
     6241 tgcgtggaag accaatatta aagacagtag cggtaagccg ctgtactaat aaaactccag
     6301 gccgggtacg gtgttttacg ccgcatccgg cattacaaaa tgactttgta aacgcgttta
     6361 actgaagagt aacttatggc tgcaaccaag cctgctttta acccaccggg taaaaagggc
     6421 gacataattt tcagcgtgct ggtaaaactg gcggcgctga ttgtgctatt gatgttgggt
     6481 ggcattattg tctctctgat catctcctcc tggccgagca ttcagaaatt tggtctggct
     6541 ttcctatgga ccaaagagtg ggatgcaccg aacgatatct acggggcgct ggtgccgatc
     6601 tacggtacgt tggtgacttc gtttatcgcg ctgctgatcg ccgtcccggt gagtttcggt
     6661 atcgccctgt tcctgactga gcttgcgcct ggctggctga aacgcccgct gggtatcgcc
     6721 attgagctgc tggcagccat tccaagtatc gtttacggca tgtggggcct gtttatcttt
     6781 gcgccgctgt tcgccgttta ctttcaggag ccggtcggca atatcatgtc gaatatcccg
     6841 attgttggcg cgctgttctc tggccccgca tttggtatcg gtatcctcgc ggcaggcgtg
     6901 atcctcgcca tcatgattat tccgtacatt gcggcggtaa tgcgtgatgt gttcgaacaa
     6961 accccggtga tgatgaaaga gtcggcctac ggtattggct gcaccacctg ggaagttatc
     7021 tggcgtatcg ttcttccgtt caccaaaaat ggtgttatcg gcggcatcat gctggggctg
     7081 ggccgcgcgc tcggtgaaac catggcggtg acctttatca tcggtaacac ctaccagctc
     7141 gacagcgcct cgctgtatat gccgggcaac agtatcacct ctgcgctggc gaacgaattt
     7201 gcggaagcgg aatccggtct gcacgttgcc gcactgatgg aactgggcct gatcctgttt
     7261 gtgattacct tcatcgtcct cgccgcatcg aagtttatga ttatgcgcct ggctaagaat
     7321 gagggggcac gctaatggct atggttgaaa tgcaaaccac tgcggcgctg gctgaatctc
     7381 gccgcaaaat gcaggcgcgt cgccgcctca aaaaccgtat tgcgctgacg ctctcgatgg
     7441 cgacgatggc cttcggcctg ttctggctga tctggatttt aatgtccacc atcactcgcg
     7501 gtatcgacgg tatgtcgctg gcgctgttca ctgaaatgac gccgccgccc aatacggaag
     7561 gtggtggtct ggcgaacgcc ctggcgggta gcgggctgtt aattttgtgg gccacggtat
     7621 tcggtacgcc gctgggcatt atggcgggga tttatctggc ggaatatggt cgtaaatcct
     7681 ggctggcaga agtgattcgc ttcattaacg acattctgct ctccgcgccg tcgattgtgg
     7741 ttggtctgtt tgtttacacc atcgtggtgg cgcagatgga gcacttctcc ggctgggcgg
     7801 gcgtgattgc cctggcgctg ttgcaggtgc cgattgttat ccgcaccacc gagaacatgc
     7861 tgaaactggt gccggacagc ctgcgtgaag cggcttatgc gctgggtaca ccgaagtgga
     7921 agatgatctc cgcgattacg ctgaaagcgt cggtgtccgg gatcatgacc ggtatcttgc
     7981 tggcgattgc ccgtattgct ggtgaaaccg cgccgctgct gtttaccgcg ctctccaacc
     8041 agttctggag cacggacatg atgcagccga tcgccaacct gccggtgacg atctttaagt
     8101 ttgcgatgag tccgtttgcg gaatggcagc aactggcctg ggccggggta ttgatcatta
     8161 ccctgtgcgt actgctgctg aacattctgg cgcgcgttgt tttcgcgaag aataaacacg
     8221 gttgatattg ctgacacggt tttcccctca ccctaaccct ctccccagag gggcgagggg
     8281 accgaccgag cgcctttttg actctgtaca cggttaacac tttgccggat gcggcgtgaa
     8341 cgcctgatcc ggcctacggt aagcctgatt agcgaagtgc atcaggcacg atgaggaaaa
     8401 gattgcaatg agtatggttg aaactgcccc gagtaaaatt caggttcgta atttgaactt
     8461 ctactacggc aaattccatg ccctgaaaaa catcaacctg gatatcgcta aaaaccaggt
     8521 aacggcgttt atcgggccgt ccggctgcgg taaatcgacg ctgctgcgta ccttcaacaa
     8581 aatgtttgaa ctgtacccgg agcagcgtgc ggaaggtgaa attctgcttg atggcgacaa
     8641 catcctgacc aactctcagg atatcgcact gctgcgtgcg aaagtgggca tggtgttcca
     8701 gaaaccgacg ccgtttccga tgtccatcta cgacaacatc gcttttggcg ttcgtctgtt
     8761 tgagaagctc tcccgtgccg acatggacga gcgcgtgcag tgggcattga ccaaagccgc
     8821 attgtggaac gaaaccaaag ataaattgca ccagagcggt tactctctct ctggtggtca
     8881 gcaacagcgt ctgtgtattg cgcgtggtat cgccattcgc ccggaagtgc tgctgctcga
     8941 cgaaccgtgt tcggcgctcg accctatctc taccgggcgt attgaagagc tgatcaccga
     9001 actgaagcag gattacaccg tggtgatcgt cacccacaac atgcagcagg ctgcgcgttg
     9061 ttccgaccac acggcgttta tgtacctggg cgaattgatt gagttcagca acacggacga
     9121 tctgttcacc aagccagcga agaaacaaac agaagactac atcaccggtc gttacggttg
     9181 attcaggagt gcgttatgga cagtctcaat cttaataaac atatttccgg ccagttcaac
     9241 gccgaactgg aaagtatccg cacgcaggtg atgaccatgg gcggcatggt ggagcagcag
     9301 ctttctgatg caatcaccgc gatgcataac caggacagcg atctggcgaa gcgcgtgatc
     9361 gaaggcgaca agaacgtcaa catgatggaa gtggcgatcg atgaagcctg cgtgcgcatt
     9421 atcgccaaac gtcagccgac ggcgagcgac ctgcgactgg ttatggtgat cagtaaaacc
     9481 attgccgagc tggagcgtat tggcgacgtg gcggacaaaa tctgccgtac tgcgctggag
     9541 aaattctccc agcagcatca gccgttgctg gtaagtctgg agtcgctggg ccgtcatacc
     9601 atccagatgc tgcacgacgt gctggacgcg ttcgcgcgga tggacattga cgaagcggta
     9661 cgtatttatc gtgaagataa aaaagtcgat caggaatacg aaggtattgt tcgtcaactg
     9721 atgacctaca tgatggaaga ttcgcgtacc attccgagcg tacttactgc gctgttctgc
     9781 gcgcgttcta tcgaacgtat tggcgaccgc tgccaga
//
LOCUS       DAOFQD010000164         9161 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.164, whole genome shotgun sequence.
ACCESSION   DAOFQD010000164 DAOFQD010000000
VERSION     DAOFQD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.164"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(209..709)
                     /locus_tag="QBF46_004237"
     CDS             complement(209..709)
                     /locus_tag="QBF46_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6676348.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(837..1676)
                     /gene="sul1"
                     /locus_tag="QBF46_004238"
     CDS             complement(837..1676)
                     /gene="sul1"
                     /locus_tag="QBF46_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDQ6676349.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(1670..2017)
                     /locus_tag="QBF46_004239"
     CDS             complement(1670..2017)
                     /locus_tag="QBF46_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDQ6676350.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(2181..2972)
                     /gene="aadA1"
                     /locus_tag="QBF46_004240"
     CDS             complement(2181..2972)
                     /gene="aadA1"
                     /locus_tag="QBF46_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HDQ6676351.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGRIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(3065..3538)
                     /locus_tag="QBF46_004241"
     CDS             complement(3065..3538)
                     /locus_tag="QBF46_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HDQ6676352.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3695..4813
                     /locus_tag="QBF46_004242"
     CDS             3695..4813
                     /locus_tag="QBF46_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HDQ6676353.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDGVVCGR
                     GRNPTLRLWPTIFSGKIDVFPGDRRSRLISSMTSVAPA"
     gene            complement(4785..5621)
                     /locus_tag="QBF46_004243"
     CDS             complement(4785..5621)
                     /locus_tag="QBF46_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDQ6676354.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5621..6424)
                     /locus_tag="QBF46_004244"
                     /note="aph(3'')-Ib"
     CDS             complement(5621..6424)
                     /locus_tag="QBF46_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDQ6676355.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(6485..7300)
                     /gene="sul2"
                     /locus_tag="QBF46_004245"
     CDS             complement(6485..7300)
                     /gene="sul2"
                     /locus_tag="QBF46_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDQ6676356.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(7609..8460)
                     /locus_tag="QBF46_004246"
     CDS             complement(7609..8460)
                     /locus_tag="QBF46_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDQ6676357.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRPKAAG"
     gene            complement(8447..>9161)
                     /locus_tag="QBF46_004247"
     CDS             complement(8447..>9161)
                     /locus_tag="QBF46_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6676358.1"
                     /translation="KSMLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHR
                     LHALGAHLSAEERQAVADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRR
                     FHIEEENASGPMAQVIGRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVL
                     VDNIRWQSYLSSMTSAEAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVL
                     KPAVLERQRKSKGVPRGEA"
BASE COUNT         1973 a         2685 c         2600 g         1903 t
ORIGIN      
        1 aaacagaccc gcaaactgaa aatcgagaca taattctgac aacaaggggt gggtcaaaat
       61 tcaatgcaaa tcccgggtca aatttgggtg caaatcaaca gatatcgaca acctctcgcg
      121 caaccaagac atcgcggtcg gactgcaagt gatcttgaag ccacgggccc gtcccacccc
      181 gacatggacc tcgatgcccg aacggacgtt agatttcgag ttctaggcgt tctgcgatga
      241 aggttggatc ccagccggga ttgaaagtgt cgacgtgggt gaatccgagc cgctcgtata
      301 ggccacgcag gttcgggtgg cagtcgagcc gcagcttggc gcacccctgc gttcgcgcgg
      361 catggcggca agcctcgatc agcgcggagc tgacaccccg gcccgcatgt gtccgtcgca
      421 ccgcgagctt gtgcagatat gcggcctccc ccttgagggc gtcgggccag aactcgggat
      481 cctcggccga caaggtgcaa cagccgacga tgccgtcgct gcaactcgcg actaggagct
      541 cggatctcag gacgaaggtc tccgcgaatg tccggtcgat ccgcgcgacg tcccaggcgg
      601 gcgttccctt ggcggacatc cacgccgcag cgtcgtgcat cagccgcaca acctcgtcga
      661 tatcacccga gcaggcgacc cgaacgttcg gaggctcctc gctgtccatt cgctcccctg
      721 gcgcggtatg aaccgccgcc tcatagtgca gtttgatcct gacgagccca gcatgtctgc
      781 gcccaccttc gcggaacctg accagggtcc gctagcgggc ggccggaagg tgaatgctag
      841 gcatgatcta accctcggtc tctggcgtcg cgactgcgaa atttcgcgag ggtttccgag
      901 aaggtgattg cgcttcgcag atctccaggc gcgtgggtgc ggacgtagtc agcgccattg
      961 ccgatcgcgt gaagttccgc cgcaaggctc gctggaccca gatcctttac aggaaggcca
     1021 acggtggcgc ccaagaagga tttccgcgac accgagacca atagcggaag ccccaacgcc
     1081 gacttcagct tttgaaggtt cgacagcacg tgcagcgatg tttccggtgc ggggctcaag
     1141 aaaaatccca tccccggatc gaggatgagc cggtcggcag cgaccccgct ccgtcgcaag
     1201 gcggaaaccc gcgcctcgaa gaaccgcaca atctcgtcga gcgcgtcttc gggtcgaagg
     1261 tgaccggtgc gggtggcgat gccatcccgc tgcgctgagt gcataaccac cagcctgcag
     1321 tccgcctcag caatatcggg atagagcgca gggtcaggaa atccttggat atcgttcagg
     1381 tagcccacgc cgcgcttgag cgcatagcgc tgggtttccg gttggaagct gtcgattgaa
     1441 acacggtgca tctgatcgga cagggcgtct aagagcggcg caatacgtct gatctcatcg
     1501 gccggcgata caggcctcgc gtccggatgg ctggcggccg gtccgacatc cacgacgtct
     1561 gatccgactc gcagcatttc gatcgccgcg gtgacagcgc cggcggggtc tagccgccgg
     1621 ctctcatcga agaaggagtc ctcggtgaga ttcagaatgc cgaacaccgt caccatggcg
     1681 tcggcctccg cagcgacttc cacgatgggg atcgggcgag caaaaaggca gcaattatga
     1741 gccccatacc tacaaagccc cacgcatcaa gcttttgccc atgaagcaac caggcaatgg
     1801 ctgtaattat gacgacgccg agtcccgacc agactgcata agcaacaccg acagggatgg
     1861 atttcagaac cagagaaaga aaataaaatg cgatgccata accgattatg acaacggcgg
     1921 aaggggcaag cttagtaaag ccctcgctag attttaatgc ggatgttgcg attacttcgc
     1981 caactattgc gataacaaga aaaagccagc ctttcatgat atatctccca atttgtgtag
     2041 ggcttattat gcacgcttaa aaataataaa agcagacttg acctgatagt ttggctgtga
     2101 gcaattatgt gcttagtgca tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg
     2161 cttgaacgaa ttgttagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag
     2221 tgaacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga
     2281 taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
     2341 cagtcggcag cgacatcctt cggcgcgatt ctgccggtta ctgcgctgta ccaaatgcgg
     2401 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
     2461 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
     2521 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
     2581 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
     2641 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
     2701 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
     2761 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
     2821 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
     2881 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
     2941 gtcgatactt cggcgatcac cgcttccctc atgatgttta acgcctggca cagcggatcg
     3001 caaacctggc gcggcttttg gtacaaaagg cgtgacaggt ttgcgaatcc gttgctgcca
     3061 cttgttaacc cttttgccag atttggtaac tataatttat gttagaggcg aagtcttggg
     3121 taaaaactgg cctaaaattg ctggggattt caggaaagta aacatcacct tccggctcga
     3181 tgtctattgt agatatatgt agtgtatcta cttgatcgat caggcttttg tatatctccc
     3241 caccacctga aacaatgaca tgatccgtta ttttctttag gttggttaaa gcatctttaa
     3301 ttgatggaaa gatcactacg ttctcattgt cagatgtaaa acttgaacgt gttacgaccg
     3361 catactttcg gttgggtaat gctcccattg attcaaaagt cttgcgtcca accaacagcc
     3421 attggttata ggtaatagct ttaaacagga gctgttcacc tttggcactc catggaatat
     3481 cagggccatt cccgataact ccattcttcg atatagctac cattagtgat agtttcacaa
     3541 ttcttcctca gaggttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
     3601 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
     3661 catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
     3721 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta
     3781 cagcttacga accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg
     3841 tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc
     3901 gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt
     3961 ctacggcaag gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc
     4021 gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct
     4081 ggaaggcgag catcgtttgt tcgcccagct tctgtatgga acgggcatgc ggatcagtga
     4141 gggtttgcaa ctgcgggtca aggatctgga tttcgatcac ggcacgatca tcgtgcggga
     4201 gggcaagggc tccaaggatc gggccttgat gttacccgag agcttggcac ccagcctgcg
     4261 cgagcagctg tcgcgtgcac gggcatggtg gctgaaggac caggccgagg gccgcagcgg
     4321 cgttgcgctt cccgacgccc ttgagcggaa gtatccgcgc gccgggcatt cctggccgtg
     4381 gttctgggtt tttgcgcagc acacgcattc gaccgatcca cggagcggtg tcgtgcgtcg
     4441 ccatcacatg tatgaccaga cctttcagcg cgccttcaaa cgtgccgtag aacaagcagg
     4501 catcacgaag cccgccacac cgcacaccct ccgccactcg ttcgcgacgg ccttgctccg
     4561 cagcggttac gacattcgaa ccgtgcagga tctgctcggc cattccgacg tctctacgac
     4621 gatgatttac acgcatgtgc tgaaagttgg cggtgccgga gtgcgctcac cgcttgatgg
     4681 ggtcgtttgc gggagagggc gaaatcctac gctaaggctt tggccaacga tattctccgg
     4741 taagattgat gtgttcccag gggataggag aagtcgcttg atatctagta tgacgtctgt
     4801 cgcacctgct tgatcgcggc cgcgatagct agatcgcgtt gctcctcttc tccatccgcg
     4861 ttccaagctg cggaaaggca cccataagcg tacgcctggt cgagcaggcg acgcggatcg
     4921 acgtccagcg cacgagagaa tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa
     4981 aggtcgtctc tgtcagccgg atcgtagaac atattggcgg cgccaaagcc cacttcaccg
     5041 accagaccga cgggatctat caccagccag ccgcgactgg agaacatgat gttttcatga
     5101 tgcagatcgc catgtagccc acgcagttcc gaggcattgc tcatcatttg atcggctata
     5161 atcgccgcgt ggacgtagtc agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga
     5221 aacaaagctg caaagcgatc ccggatcggg agaagggcag aaggcagggg ttcctcagat
     5281 gcggcataca gcttcgccat tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc
     5341 tcggcaacga tgtgagagag cattcgctcc ccggcatatt cgagcaacat cagattgttc
     5401 tcacgaccga gcaaccggac tgctcccctc ccattgcgcc ataccagata gtcggccccg
     5461 cgcagttcat cagcaatgtc ttctataggt ttcaatccct tgacgattgc aggagtcccg
     5521 tctggcaatg aaactttcca aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc
     5581 gaaacgtgcc aatgagcagg aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt
     5641 ccaatcgcag atagaaggca aggcgttcgc ggtcgggggc ttcgatcccc aatacattga
     5701 ataggacagc gaaggcgcgc tctgcttcat ctggcgctgc ccagttctct tcggcgttag
     5761 caatcatgag tgccaaatcg gcatagcgat ctgctgttcc gagccgccca aggtcgatca
     5821 gacccgtgca ttgaagagtt ttagggtcca ccatgaagtt cggcatgcag ggatcaccat
     5881 ggcaaacaac catatcggtg cgctcttggt cgagccgcac cggtagctct cgttcgacac
     5941 gagccaaaag atcgagctgc ggcgtactct tgtcctcgtc cggtaagaag tcgggattga
     6001 cggcattgcg ggacaccaca tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa
     6061 acggacattg atcaaccgat aggctgtgaa cagcgccaag ttgctgcccc attgacggcc
     6121 acgctttgag caaatccgct ccagacagat cagccgccgg tactcccgga attgccgtta
     6181 tcaccaagca tgcaccctcc tgttcctcct gccagttgat gacctcgggg caagccacac
     6241 ctcgaccttt gagccaaatg aggcggtcac gctctccagc gagctcaccg cggcgggaag
     6301 caggtgcgat tttcgcgaag gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt
     6361 ctccgcctct gacaggcaac cagtcagaat gcgattcacc aaaaaaaata ttagttcgat
     6421 tcaatggagg ttccttcagt tttctgatga agcgcgaata tagagaaata tcccgaatgt
     6481 gcagttaacg aattcttgcg gtttctttta gcgccgccaa taccgccagc ccgtcgcgca
     6541 aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc tgcggcggcg gcaagctctg
     6601 cagcgagtgt cgcggccccg acatcccccg gaccacggcc tgtgagcgcg cgcagaaagg
     6661 atttgcgcga aacagacaga agcaccggca aatcgaagcg cagccgcaat tcatcgaacc
     6721 gcgccagcac cgagagcgag gtttcgggag cagcccccag aaaaaacccc atgccgggat
     6781 caaggacaag gcggttgcgt ttgataccgg cacccgtcag cgccgcgatg cgcgcgtcaa
     6841 agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc ctcgcgccga tctgcctgcc
     6901 cgtcttgcac cgaatgcata acgacgagtt tggcagatga tttcgccaat tgcggataga
     6961 acgcagcgtc tggaaaaccg cgaatatcat tgagataggc cacaccacgc gacaaggcat
     7021 aggcttgcgt cgcgggttga taactgtcga gcgagacggg aatgccatct gccttgagcg
     7081 cgtccagcac cggcgcgata cgctcgattt ctgtgtcgga cgaaacaggc gcggcgtcgg
     7141 ggttgctgga tgccggaccg aggtcgatca catctgcccc ctcggccatc agcttacgcg
     7201 cctgcgcaat ggctgcgtct ggcgccagat accggcctcc atcggagaaa ctgtccgagg
     7261 ttatgttgac gatgccgaaa atgatgagcg atttattcat gggggcttct ataataataa
     7321 taatcgagca tgagtctcat acggatgctc gggtcgaaag ggaatcccca ggcgagtaac
     7381 ctgtttgcgg tgatccatta gctgcaggag cagaatagca tacatctgga agcaaagcca
     7441 ggaaagcggc ctatggagct gtgcggcagc gctcagtagg caatttttca aaatattgtt
     7501 aagccttttc tgagcatggt atttttcatg gtattaccaa ttagcaggaa aataagccat
     7561 tgaatataaa agataaaaat gtcttgttta caatagagtg ggggggggtc agcctgccgc
     7621 cttgggccgg gtgatgtcgt acttgcccgc cgcgaactcg gttaccgtcc agcccagcgc
     7681 gaccagctcc ggcaacgcct cgcgcacccg ctggcggcgc ttgcgcatgg tcgaaccact
     7741 ggcctctgac ggccagacat agccgcacaa ggtatctatg gaagccttgc cggttttgcc
     7801 ggggtcgatc cagccacaca gccgctggtg cagcaggcgg gcggtttcgc tgtccagcgc
     7861 ccgcacctcg tccatgctga tgcgcacatg ctggccgcca cccatgacgg cctgcgcgat
     7921 caaggggttc agggccacgt acaggcgccc gtccgcctcg tcgctggcgt actccgacag
     7981 cagccgaaac ccctgccgct tgcggccatt ctgggcgatg atggatacct tccaaaggcg
     8041 ctcgatgcag tcctgtatgt gcttgagcgc cccaccacta tcgacctctg ccccgatttc
     8101 ctttgccagc gcccgatagc tacctttgac caccatggca tcagcggtga cggcctccca
     8161 cttgggttcc aggaacagcc ggagctgccg tccgccttcg gtcttgggtt ccgggccaag
     8221 cactaggcca ttaggcccag ccatggccac cagcccttgc aggatgcgca gatcatcagc
     8281 gcccagcggc tccgggccgc tgaactcgat ccgcttgccg tcgccgtagt catacgtcac
     8341 gtccagcttg ctgcgcttgc gctcgccccg cttgagggca cggaacaggc cgggggccag
     8401 acagtgcgcc gggtcgtgcc ggacgtggct gaggctgtgc ttgttcttag gcttcaccac
     8461 ggggcacccc cttgctcttg cgctgcctct ccagcacggc gggcttgagc accccgccgt
     8521 catgccgcct gaaccaccga tcagcgaacg gtgcgccata gttggccttg ctcacaccga
     8581 agcggacgaa gaaccggcgc tggtcgtcgt ccacacccca ttcctcggcc tcggcgctgg
     8641 tcatgctcga caggtaggac tgccagcgga tgttatcgac cagtaccgag ctgccccggc
     8701 tggcctgctg ctggtcgcct gcgcccatca tggccgcgcc cttgctggca tggtgcagga
     8761 acacgataga gcacccggta tcggcggcga tggcctccat gcgaccgatg acctgggcca
     8821 tggggccgct ggcgttttct tcctcgatgt ggaaccggcg cagcgtgtcc agcaccatca
     8881 ggcggcggcc ctcggcggcg cgcttgaggc cgtcgaacca ctccggggcc atgatgttgg
     8941 gcaggctgcc gatcagcggc tggatcagca ggccgtcagc cacggcttgc cgttcctcgg
     9001 cgctgaggtg cgccccaagg gcgtgcaggc ggtgatgaat ggcggtgggc gggtcttcgg
     9061 cgggcaggta gatcaccggg ccggtgggca gttcgcccac ctccagcaga tccggcccgc
     9121 ctgcaatctg tgcggccagt tgcagggcca gcatggattt a
//
LOCUS       DAOFQD010000165         9128 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.165, whole genome shotgun sequence.
ACCESSION   DAOFQD010000165 DAOFQD010000000
VERSION     DAOFQD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.165"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(40..660)
                     /gene="sodA"
                     /locus_tag="QBF46_004248"
     CDS             complement(40..660)
                     /gene="sodA"
                     /locus_tag="QBF46_004248"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDQ6676359.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            945..1979
                     /gene="rhaT"
                     /locus_tag="QBF46_004249"
     CDS             945..1979
                     /gene="rhaT"
                     /locus_tag="QBF46_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDQ6676360.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKSLIIHNVLLSTLGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(1976..2842)
                     /gene="rhaR"
                     /locus_tag="QBF46_004250"
     CDS             complement(1976..2842)
                     /gene="rhaR"
                     /locus_tag="QBF46_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDQ6676361.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(2898..3734)
                     /gene="rhaS"
                     /locus_tag="QBF46_004251"
     CDS             complement(2898..3734)
                     /gene="rhaS"
                     /locus_tag="QBF46_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDQ6676362.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTAGREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4022..5491
                     /gene="rhaB"
                     /locus_tag="QBF46_004252"
     CDS             4022..5491
                     /gene="rhaB"
                     /locus_tag="QBF46_004252"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDQ6676363.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARFERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKACEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            5488..6747
                     /gene="rhaA"
                     /locus_tag="QBF46_004253"
     CDS             5488..6747
                     /gene="rhaA"
                     /locus_tag="QBF46_004253"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDQ6676364.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            6989..7813
                     /gene="rhaD"
                     /locus_tag="QBF46_004254"
     CDS             6989..7813
                     /gene="rhaD"
                     /locus_tag="QBF46_004254"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDQ6676365.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            7823..8137
                     /gene="rhaM"
                     /locus_tag="QBF46_004255"
     CDS             7823..8137
                     /gene="rhaM"
                     /locus_tag="QBF46_004255"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDQ6676366.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            8438..8884
                     /locus_tag="QBF46_004256"
     CDS             8438..8884
                     /locus_tag="QBF46_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDQ6676367.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            8895..>9128
                     /locus_tag="QBF46_004257"
     CDS             8895..>9128
                     /locus_tag="QBF46_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS subunit IIBC"
                     /protein_id="HDQ6676368.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRAR"
BASE COUNT         2178 a         2379 c         2414 g         2157 t
ORIGIN      
        1 atacggcgta aacgcctcat tgcagcaggc ggcaaatgat tattttttcg ccgcgaaacg
       61 tgctgctgct tcgtcccagt tcaccacgtt ccagaactct ttgatgtagt ccggacggcg
      121 gttctggaat ttcaggtagt aagcatgttc ccacacatcc aggcccagaa tcgggaagcc
      181 ggatgcgcca gaaatggctt cacccatcag cggggagtcc tggttagcag tagaaaccac
      241 cgccagttta tcgcctttca gcaccagcca tgcccagccg gaaccaaagc gggaagctgc
      301 cgctttctca aattctgctt tgaagttatc aacggagccg aagtcacgtt cgatagccgc
      361 tttcaggtca ccctgcaggg tggtgccttt tttcagacct ttccagaaca ggctgtggtt
      421 agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg tctgctggca gctggtccag
      481 tttggtgatc agctcttcaa ccggcaggtt ggcaaattct ggcaggcttt ccagcgccgc
      541 gttggcgttg tttacgtagg tctgatggtg tttggtgtgg tggatttcca tggtctgctt
      601 atcgaagtgc ggttccaggg catcgtaagc atacggcagg gatggcaggg tatagctcat
      661 attcatctcc agtattgtcg ggcggccgat tgttaatgcc gcgtaagcag ttggttcatt
      721 atagttaatt aaatgatatt gaaaatgatt atcaatgccg tacttttcgt aagggtatgg
      781 ttttgcagga aaatgcccga gatgtgaagc aaatcaccca cttaatgccg tgattgccag
      841 taaatcgaca acggcggcaa caggcgaaag gttaatcgac agcacgattt ttacactcat
      901 ctcgtcggag atgtgacgcg acgaaaaatg atgaggataa gaagatgagt aacgcgatta
      961 cgatggggat attttggcat ttgatcggcg cggccagtgc agcctgtttt tacgctccgt
     1021 tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc agtcggtggg attgtttcgt
     1081 ggattattct gccgtgggcc atcagcgccc tgttactacc gaatttctgg gcgtattaca
     1141 gctcgtttag tctctctacg ctactgcctg tttttctgtt cggcgctatg tgggggatcg
     1201 gtaatatcaa ctacggcctg accatgcgtt atctcggcat gtcgatggga attggcatcg
     1261 ccattggcat tacgttgatt gtcggtacgc tgatgacgcc aattatcaac ggcaatttcg
     1321 atgtgttgat tagcaccgaa ggcggacgca tgacgttgct cggcgttctg gtggcgctga
     1381 ttggcgtagg gattgtaact cgcgccgggc agttgaaaga gcgcaagatg ggcattaaag
     1441 ccgaagagtt caatctgaaa aaagggctgg tgctggcggt gatgtgcggc attttctctg
     1501 ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca tgaagccgct gccgcacttg
     1561 gcgtcgatcc actgtatgtc gctctgccaa gctatgttgt catcatgggc ggcggcgcga
     1621 tcattaacct cggtttctgt tttattcgtc tggcaaaagt gaaggatttg tcgctaaaag
     1681 ccgacttctc gctggcaaaa tcgctgatca ttcacaatgt gttactctcg acactgggcg
     1741 ggttgatgtg gtatctgcaa ttctttttct atgcctgggg ccacgcccgc attccggcgc
     1801 agtatgacta catcagttgg atgctgcata tgagtttcta tgtattgtgc ggcggtatcg
     1861 tcgggctggt gctgaaagag tggaacaatg caggacgccg tccggtaacg gtgttgagcc
     1921 tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg catcggcatg gcgaattaat
     1981 ctttctgcga attgagatga cgccactggc tgggcgtcat cccggtttcc cgggtaaaca
     2041 ccaccgaaaa atagttacta tcttcaaagc cacattcggt cgaaatatca ctgattaaca
     2101 ggcggctatg ctggagaaga tattgcgcat gacacactct gacctgtcgc agatattgat
     2161 tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg
     2221 cctcatcaca aaatttatcc agcgcaaagg gacttttcag gctggccgcc agccgggtaa
     2281 tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat
     2341 ggcgattcag caacatcacc aactgcccga acagcaactc agccatttcg ttagcaaacg
     2401 acacatgctg actactttca tgctcaagct gaccgataac ctgccgcgcc tgcgccatcc
     2461 ccacgctacc taagcgccag tgtggttgcc ctgcgctggc gctaaatccc ggaatcgctc
     2521 cctgccagtc aagattcagc ttcagccgct ccgggcaata aataatattc tgcaaaacca
     2581 gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg aatataaaag agatcgccac
     2641 gggtaatgcg ataaggacga tcgttgagta catgcagacc attaccgcgc cagacaatca
     2701 ccagctcaca aaaatcatgt gtatgttcgg caaagacatc ttgcggataa cggtcagcca
     2761 cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg
     2821 ccaccgtggc tacctcggcc agagaacgaa gttgattatt cgcaatatgg cgtacaaata
     2881 cgttgagaag attcgcgtta ttgcagaaag ccatcccgtc cctggcgaat atcacgcggt
     2941 gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct gaatccgcag
     3001 cgataggcga tgtcagtaac gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc
     3061 agtcgcaggc ggttcaggta acgctgaggc gtcagtcccg tttgctgctt aagctgccga
     3121 tgtagcgtac gcagcgaaag agaaaattga tccgccacgg catcccaatt cacctcatcg
     3181 gcaaaatggt cctccagcca ggccagaagc aagttgagac gtgacgcgct gttttccagg
     3241 ttctcctgca aactgctttt acgcagcaag agcagtaatt gcataaacaa gatctcgcga
     3301 ccggcggtcg agggtaaatc attttcccct tcctgctgtt ccatctgtgc aaccagctgt
     3361 cgcacctgct gcaatacgct gtggttaacg cgccagtgag acggatactg cccatcctgc
     3421 tcttgtggca gcaactgatt cagcccggcg agaaactgaa atcgatccgg cgagcgatac
     3481 agcacattgg tcagacacag attatcggta tgttcataca gatgccgatc atgatcgcgt
     3541 acgaaacaga ccgtgccacc ggtgatggta tagggctgcc cattaaatac atgaataccc
     3601 gtgccatgtt cgacaatcac aatttcatga aaatcatgat gatgttcagg aaaatccgcc
     3661 tgcgggagcc ggggttctat cgccacggac gcgttaccag acggaaaaaa atccacacta
     3721 tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt aaccacgagg
     3781 tcaggttctt accttaaatt ttcgacgaga aaccacgtaa aaaacgtcga tttttcaaga
     3841 tacagcgtga attttcagga aatgcggtga gcatcacacc accacaattc aacaaattgt
     3901 gaacatcatc acgttcatct ttccctggtt gccaatgacc cattttcctg tcagtagcga
     3961 aaaggtcgcg aattcaggcg ctttttagac tggtcgtaat gaaattcagc aggatcacat
     4021 tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca tccagtgggc gcgtgatgct
     4081 ggcgcgtttc gagcgtgaat gccgtagcct gacgctgcgc gaaatccatc gttttaacaa
     4141 tgggctgcat agccagaacg gttatgtcac ctgggatgtg gatagcctgg aaagtgccat
     4201 tcgccttgga ttaaacaagg cgtgcgagga agggattcgt atcgatagca ttgggattga
     4261 tacctggggc gtggactttg tgctgctcga ccaacagggt cagcgtgtgg gcctgcccgt
     4321 tgcttatcgc gatagccgca ccaatggcct gatggcgcag gcacagcaac aactcggcaa
     4381 acgcgatatt tatcaacgta gcggcatcca gtttctgccc ttcaatacgc tttatcagtt
     4441 gcgtgcgctg acggagcaac aacctgaact tattccacac attgctcacg ctctgctgat
     4501 gccggattac ttcagttatc gcctgaccgg caagatgaac tgggagtaca ccaacgccac
     4561 gaccacgcaa ctggtcaata tcaatagcga cgactgggac gagtcgctac tggcgtggag
     4621 cggtgccaac aaagcctggt ttggtcgccc gacgcatccg ggtaatgtca taggtcactg
     4681 gatttgccca cagggtaatg agattccggt ggtcgccgtt gccagccatg ataccgccag
     4741 cgcggttatc gcctcgccgt taaacggttc acgcgccgct tatctctctt ctggcacctg
     4801 gtcattgatg ggttttgaaa gccagacgcc atttaccaat gacacggcgc tggcagccaa
     4861 catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg ctgaaaaata ttatgggctt
     4921 atggctgctt cagcgagtgc ttcaggagcg gcaaatcaac gatcttccgg cgcttatcgc
     4981 cgcgacacag gcacttccgg cctgccgctt catcatcaat cccaatgacg atcgctttat
     5041 taatcctgag gcgatgtgca gcgaaattca ggctgcgtgt cgggaaacgg cgcaaccgat
     5101 cccggaaagt gatgctgaac tggcgcgctg cattttcgac agtctggcgc tgctgtatgc
     5161 cgatgtgttg catgagctgg cgcagctgcg cggtgaagat ttctcgcaac tgcatattgt
     5221 cggcggcggc tgccagaaca cgctgctcaa ccagctatgc gccgatgcct gcggtattcg
     5281 ggtgatcgcc gggcctgttg aagcctcgac gctcggcaat atcggcatcc agttaatgac
     5341 gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc gtcagcacta ccgcgaatct
     5401 gaccaccttt acccctaatc ctgacagtga aattgcccac tatgtggcgc agattcactc
     5461 tacacgacag acaaaggagc tttgcgcatg accactcaac tggaacaggc ctgggagctg
     5521 gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg aggcgctgcg ccaacttgat
     5581 cgtttacccg tttcaatgca ctgctggcag ggcgatgatg tttccggttt tgaaaacccg
     5641 gaaggttcgc tgaccggtgg gattcaggct actggtaatt atccgggcaa agcgcgtaat
     5701 gccagtgagc tgcgtgccga tctggaacag gctatgcggc tgattccggg gccgaaacgg
     5761 cttaatttac atgccatcta tctggaatca gatacgccag tctcgcgcga ccagatcaaa
     5821 ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca atcagctcgg tctggatttt
     5881 aacccctcct gcttttcgca tccgctaagc gccgatggct ttacgctttc ccatgccgac
     5941 gacagcattc gccagttctg gattgatcac tgcaaagcca gccgtcgcgt ttcggcctat
     6001 tttggcgagc aactcggcac accatcggtg atgaacatct ggatcccgga tggtatgaaa
     6061 gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc tggcagcact ggatgaggtg
     6121 atcagcgaga agctaaaccc tgcgcaccat atcgacgccg ttgagagcaa attgtttggc
     6181 attggcgcgg agagctacac ggttggctcc aatgagtttt acctggggta tgccaccagc
     6241 cgccagacgg cgctgtgcct ggacgccggg catttccacc cgactgaagt gatttccgac
     6301 aagatttccg ccgccatgct gtatgtgccg cagttgctgc tgcacgtcag ccgtccggtt
     6361 cgctgggaca gcgatcacgt agttctgctg gatgatgaaa cccaggcaat tgccagtgag
     6421 attgttcgtc acgatctgtt tgaccgggtg catatcggcc tggatttctt tgatgcctct
     6481 atcaaccgca ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc cctgctgcgt
     6541 gcgttgctgg aacctaccgc cgagctgcgc aagctggaag cggcgggcga ttacactgcg
     6601 cgtctggcgc tactggaaga gcagaaatcg ttgccgtggc aggcggtctg ggaaatgtat
     6661 tgtcaacgtc atgatacgcc agcaggtagc gaatggctgg agagcgtgcg ggcttatgag
     6721 aaagcgattt tgagccagcg tgggtaaaca ctgccggatg cggcgcgagc gccttatccg
     6781 gcctacaggt cgaatgacca tgcgtgctgc caccctcacc ccggccctct ccctgaaagg
     6841 gcgagggaga aaaacatagc ctcgtgcggc aacggagctg aacaccgctc cggacagtcc
     6901 cctcgcccct acggggagag ggttagggtg aggggaacaa gccgcaccat gtctaaacaa
     6961 ggcaacctaa tttacaggaa cacagaacat gcaaaacatt actcagtcct ggtttgtcca
     7021 gggaatgatc aaagccacca ccgacgcctg gctgaaaggc tgggatgagc gcaacggcgg
     7081 caacctgacg ctacgcctgg atgacgccga tatcgcacca tatcacgaca atttccacca
     7141 acaaccgcgc tatatcccgc tcagccagcc catgccttta ctggcaaata caccgtttat
     7201 tgtcaccggc tcgggcaaat tcttccgtaa cgtccagctt gatcctgcgg ctaacttagg
     7261 catcgtaaaa gtcgacagcg acggcgcggg ctaccacatt ctttgggggt taaccaacga
     7321 agccgttccc acttccgaac ttccggctca cttcctttcc cactgcgagc gcattaaagc
     7381 caccaacggc aaagatcggg tgatcatgca ctgccacgcc accaacctga tcgccctcac
     7441 ctatgtactg gaaaacgaca ccgcggtctt cactcgccaa ctgtgggaag gcagcaccga
     7501 gtgtctggtg gtattcccgg atggcgttgg cattctgccg tggatggtac ccggcacgga
     7561 cgaaatcggc caggcgaccg cgcaagagat gcaaaaacat tcgctggtgt tgtggccctt
     7621 ccacggtgtg ttcggttccg gaccgacgct ggatgaaacc ttcggtttaa tcgacaccgc
     7681 agaaaaatca gcacaagtat tagtgaaggt ttattcgatg ggcggcatga aacagactat
     7741 cagccgtgaa gagttgatag cgctcggcca gcgtttcggc gttacgccac tagccagtgc
     7801 gctggcgctg taaggagcaa acatgatccg caaagccttt gtcatgcagg ttaaccccga
     7861 cgcccacgaa gagtatcagc gtcggcataa ccccatctgg ccggaactgg aagcagtgtt
     7921 gaaatctcac ggtgcgcata actacgccat ttacctcgac aaagcacgta atctgctgtt
     7981 tgccatggta gagattgaat ctgaagaacg gtggaatgcg gtagccagta ctgatgtttg
     8041 ccaacgttgg tggaaatata tgaccgatgt tatgcccgct aacccggata acagcccggt
     8101 gagtagcgag ctgcaagaag tgttttacct gccgtaattg cccaatgtgc cggagatgcc
     8161 tccggcacaa tattttaaat ttcgatttga aaaattaaac atattacatc tcccggtaag
     8221 ttatatttcc ctgatacatt gtgagtaaat cacaaaaata atgaataacc cattaatgat
     8281 tcatgtggtt tatttaaata accattatgt gcattactcc gcaaatctga cctttcactc
     8341 tgtctcaaat tgtcattgtt taccgacata tcgcctctca tcacttaatc gctccaccat
     8401 tacaattgat taaaatatat tttatggagt ccgattaatg gcagctctta ctgcaagctg
     8461 tattgacctg aatattcagg gcaatggcgc ttattccgtt ctgaagcagt tggcgacaat
     8521 agcgttacaa aacggtttta tcaccgactc acaccagttc ctgcaaaccc tgctgctgcg
     8581 cgaaaaaatg cactctactg gatttggttc cggtgtcgcc gtgccgcacg gtaaaagcgc
     8641 ctgcgttaaa caaccgttcg tattattcgc ccgcaaagcg caggctattg actggaaagc
     8701 cagcgatggc gaagacgtca attgctggat ctgcctcggc gtgccgcaaa gcggcgaaga
     8761 ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt atccaccagg attttattca
     8821 tcaactgcaa cagggcgacg ccgaccaggt gcttgccttg ttaaatcaaa ccctcagctc
     8881 ataaggaagt ggcgatggag tcatccttac gtattgtcgc gatcaccaac tgccccgccg
     8941 ggatcgctca cacctacatg gtggcggaag ccctggaaca gaaagcccgt tctctcggtc
     9001 ataccattaa agttgaaact caaggatcaa gcggcgtcga aaaccgctta tccagcgaag
     9061 agattgccgc tgccgattac gtcattctcg ctaccgggcg tggcctgagc ggtgatgatc
     9121 gcgcgcgg
//
LOCUS       DAOFQD010000166         8927 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.166, whole genome shotgun sequence.
ACCESSION   DAOFQD010000166 DAOFQD010000000
VERSION     DAOFQD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.166"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..534)
                     /gene="pabA"
                     /locus_tag="QBF46_004258"
     CDS             complement(<1..534)
                     /gene="pabA"
                     /locus_tag="QBF46_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDQ6676369.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGH"
     gene            complement(566..1168)
                     /locus_tag="QBF46_004259"
     CDS             complement(566..1168)
                     /locus_tag="QBF46_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDQ6676370.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(1158..1325)
                     /locus_tag="QBF46_004260"
     CDS             complement(1158..1325)
                     /locus_tag="QBF46_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDQ6676371.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(1430..2002)
                     /gene="ppiA"
                     /locus_tag="QBF46_004261"
                     /gene_synonym="rotA"
     CDS             complement(1430..2002)
                     /gene="ppiA"
                     /locus_tag="QBF46_004261"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDQ6676372.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            2273..3454
                     /gene="tsgA"
                     /locus_tag="QBF46_004262"
     CDS             2273..3454
                     /gene="tsgA"
                     /locus_tag="QBF46_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDQ6676373.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            3716..6259
                     /gene="nirB"
                     /locus_tag="QBF46_004263"
     CDS             3716..6259
                     /gene="nirB"
                     /locus_tag="QBF46_004263"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDQ6676374.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVLCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            6256..6582
                     /gene="nirD"
                     /locus_tag="QBF46_004264"
     CDS             6256..6582
                     /gene="nirD"
                     /locus_tag="QBF46_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDQ6676375.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            6708..7514
                     /gene="nirC"
                     /locus_tag="QBF46_004265"
     CDS             6708..7514
                     /gene="nirC"
                     /locus_tag="QBF46_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDQ6676376.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFSLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            7533..8906
                     /gene="cobA"
                     /locus_tag="QBF46_004266"
     CDS             7533..8906
                     /gene="cobA"
                     /locus_tag="QBF46_004266"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDQ6676377.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDADLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         2072 a         2325 c         2355 g         2175 t
ORIGIN      
        1 atgtccttgt tcgctaagaa tactttctgg atggaactgc acaccttcca gatcccactg
       61 gcgatggcga atccccataa tctcgcgggt ttcgctccag gccgtcacgt caaagcacgc
      121 tggtaatgag tcaggttcca ccaccagcga atggtagcgt gtcacggtaa gtggatttgc
      181 cagcccccga aatacgccct caccgttatg tgtaatcggc gaggttttgc cgtgcatgac
      241 ctttgcggcg cgcacaactt taccgccaaa tgcctgcgcc attgcctgat gaccgaggca
      301 gacgccaaga atcggcaagc gcccggcata gtggcgaata acgtcaaggg agatcccggc
      361 ttcatctggc gtacaggggc caggtgagat gacaattttt tgtggtttaa gggcgtcgat
      421 atccgccagc gtcaacgcat cgttgcgctt aaccagcaca tccgccccca gttcacaaaa
      481 gtactggtag aggttccagg taaaagaatc gtagttatct ataagcagga tcatggcggc
      541 tccggtacaa aagaaccgcg ctattttact cagattcccc ggcttcgctt accactttgc
      601 taaatatcat ctgcagtgcg gtgagatccc ccattgcccc actctgattt gcctgattcc
      661 aggcctcttt ttcgataccc tgccagctca gttgatatcc ggcatgaatc gccagttgct
      721 cgaagaagat ccgctgtgcc agaccacttc ccacccggaa gggatgcagc acgttgattt
      781 cacaatagta atgcgccagc cgctcgacga acttcgcttt ctccaggcca accagataac
      841 cttcttcctc cagatcctgc atcagggcat tgccctcttt ttcgatataa gcaaagtggc
      901 agaacggcgt atcaccctga taaatatcaa cttcacgcag ttgccccgcc cagtcgaaaa
      961 tatcctgata cagctggcga tggatagtgc gcaaatgcgg taaaccgcgc accagcggac
     1021 caagctcaat ggtcgcagca cgcagcgccg tcatttcgta agcggcctgt tccagccgct
     1081 gctgctggtg gatgttcagc cggttacgca tgatatcaag gccaggataa agatacggat
     1141 cgcgcccttc gccgaattta tcgctcatag tgcctcctca gctcttcaag gcgcgctaaa
     1201 gcctctgcgg cagtaagagt gactaaaggc atctcgacgc cttcaagacg gcgactggcc
     1261 tggaaattac gattacgctg aagctcccag agacgggact tttgcttatc ggtgagtttc
     1321 ttcacttgat gcctccctga acgtgtccat ttgccacaag tataagcggc aaatccgggt
     1381 tacgccggga gagcagaagc gcgggcaaga ttgcccgcgc gaaaaatcat tacggcagga
     1441 ctttagcgga aaggataact accggttttg acggcacatt ctggtacgga ccaacgtcat
     1501 gagtcggcac ctgggaaatc ttatcggcaa cgtccatgcc tttcaccact ttaccaaata
     1561 ccgcgtaacc gaaatcacgc tgaccatggt caaggaaggc gttatcggca acgttgataa
     1621 agaactggct ggtggcgctg tctttgtcag cggtacgtgc catcgcgatg gtgccacgcg
     1681 tgttgcgcag gccgttatcg gcttcatttt tgattggcgg gtttggtttt ttctgctgca
     1741 tctgctcggt gaaaccgccg ccctgaatca taaagccagg aatgacgcgg tgaaaggtag
     1801 tgttgttata aaaaccgctg ttcacataat cgacaaagtt ttgcacagac actggcgctt
     1861 tttgtttatc cagctccagt tcgatgttac cagctgaggt tgtcaacaat acgtgcgggt
     1921 cccctttcgc tgccattgct gcgggagaaa gagcagaaag agcgaaaaca gcagccatcg
     1981 ccgccagggt cgatttgaac atgagatttc cttaacaaac agagcagaaa aaaagcaagt
     2041 gaagagattc taaatagcct tcaagaacaa ggccatccct ttacctaatt ttacgtatct
     2101 gaaatatatg taacctgacg atcacttttt attccgtgat caaaatcacc tcttaaaatg
     2161 caatttagca accgattgca ataaaacatt taaacagatc acaaaatcac ctaaaatcgc
     2221 ccgctcgcgg catatcccgc tatgccattt tatttttgaa cgcaggccaa tcatgactaa
     2281 cagcaatcgc atcaagctca catggattag ctttctctcc tacgcactga ctggtgcgtt
     2341 ggttattgtc accgggatgg tgatgggaaa tatcgccgat tatttcaatc tgcctgtttc
     2401 cagtatgagt aataccttca ccttcctcaa cgccggcatt ttaatctcta tcttcctcaa
     2461 cgcctggctg atggaaatcg tcccgttgaa aacgcagtta cgttttggct ttctcctgat
     2521 ggtgctggcg gttgccggtt tgatgttcag ccacagcctg gcgctgttct cggcggcgat
     2581 gttcattctc ggggtggtca gcggcatcac catgtcgatt ggtacattcc tggtaacaca
     2641 aatgtatgaa gggcgtcagc gcggttcccg cctgttattt accgactcct tcttcagtat
     2701 ggctgggatg attttcccaa tgatcgccgc gtttctactg gcgcgcagca ttgagtggta
     2761 ctgggtttat gcctgcatcg ggctggtgta tgtcgctatt tttattctga ccttcggctg
     2821 tgagttcccg gcgctgggca aacatgcgcc aaaaacggat gctccggtag agaaagaaaa
     2881 gtgggggatc ggcgtactgt ttctctctgt tgcggcgttg tgctacatcc tcggtcagtt
     2941 aggttttatc tcctgggtgc ctgagtatgc caaaggcctg ggcatgagcc tgaacgacgc
     3001 gggcacgctg gtgagtaact tctggatgtc atacatggtc ggcatgtggg cgttcagctt
     3061 tattcttcgc ttctttgatt tgcaacgcat tctgaccgta ctggctggtc tggctgcgat
     3121 tctgatgtac gtctttaaca ccggaacacc agcacatatg gcgtggtcaa ttctcgctct
     3181 gggcttcttc tccagcgcga tctataccac catcatcacc ctgggttcac agcagaccaa
     3241 agtaccgtcg ccaaaactgg ttaactttgt cctgacctgc ggaaccatcg gtactatgtt
     3301 gacctttgtg gttaccggcc cgatcgttga acatagcggt ccgcaggcgg cactgcttac
     3361 agcaaacggt ctgtacgctg tcgtctttgt gatgtgcttc ctgttaggtt tcgtcagccg
     3421 tcaccgtcag cataacaccc tgacctctca ttaattgctc atgccggacg gcactatcgt
     3481 cgtccggcct tttcctctct tcccccgcta cgtgcatcta tttctataaa cccgctcatt
     3541 ttgtctattt tttgcacaaa catgaaatat cagacaattc cgtgacttaa gaaaatttat
     3601 acaaatcagc aatatacccc ttaaggagta tataaaggtg aatttgattt acatcaataa
     3661 gcggggttgc tgaatcgtta aggtaggcgg taatagaaaa gaaatcgagg caaaaatgag
     3721 caaagtcaga ctcgcaatta tcggtaacgg tatggtcggc catcgcttta tcgaagatct
     3781 tcttgataaa tctgatgcgg ccaactttga tattaccgtt ttctgtgaag aaccgcgcat
     3841 cgcttatgac cgcgtacacc tctcgtctta cttctctcac cacaccgccg aagagctgtc
     3901 gctggtgcgc gaaggcttct acgagaaaca cggcatcaaa gttctggtcg gcgaacgcgc
     3961 tatcaccatc aaccgtcagg agaaggtgat tcactccagc gccggacgta ccgtttttta
     4021 tgacaagctg atcatggcaa ccggttccta cccgtggatc ccgccaatca aaggttctga
     4081 tactcaggac tgctttgtct atcgcactat tgaagacctc aatgccattg aatcctgcgc
     4141 ccgtcgcagc aaacgcggtg ccgttgttgg tggcggcctg ttaggtctgg aagccgcagg
     4201 cgcgctgaaa aacttaggta ttgaaaccca cgttatcgaa tttgccccta tgctgatggc
     4261 agaacagctt gatcagatgg gcggcgagca gctgcgtcgc aaaatcgaaa gtatgggcgt
     4321 gcgcgttcac accagcaaaa acacccttga gattgtgcag gaaggtgttg aagcgcgtaa
     4381 aaccatgcgt tttgccgacg gcagcgaact ggaagtcgac tttatcgtct tctctaccgg
     4441 tatccgtccg cgcgataagc tggcaaccca gtgtggtctg gacgttgctc cgcgtggggg
     4501 tattgtcatt aatgattcct gccagacttc cgatccggat atctacgcca tcggtgaatg
     4561 cgcaagctgg aacaaccgtg tatttggtct ggtagcacct ggctacaaaa tggcgcaggt
     4621 cgccgttgac catattctcg gtagcgaaaa cgcctttgaa ggtgctgacc ttagcgccaa
     4681 gctgaaactg ctgggcgtag acgtaggcgg tattggtgat gcgcacggtc gcacgcctgg
     4741 cgcacgtagc tacgtttacc tcgacgaaag taaagagatc tacaaacgcc tgattgtcag
     4801 cgaagacaac aaaaccctgc tcggtgcggt actggtgggc gataccagcg actacggtaa
     4861 cctgctgcaa ctggtgctga acgctatcga actgccggaa aacccggatt ccctgatcct
     4921 gccagcacac tcgggtagcg gcaagccgtc tatcggtgtt gataaactgc cggacagcgc
     4981 gcaaatctgc tcctgcttcg acgtcaccaa aggtgatctg attgctgcca tcaacaaagg
     5041 ctgccacaca gttgcggcgc tgaaagctga aaccaaagcg ggtactggct gcggtggctg
     5101 tatcccgctg gtcactcagg tactgaacgc ggaactggcg aaacagggca tcgaagttaa
     5161 caacaacctg tgcgaacact ttgcttattc gcgtcaggaa ctgttccatt tgatccgcgt
     5221 tgaaggcatt aaaaccttcg aagaactgct ggcgaaacac ggcaaaggct acggttgtga
     5281 agtttgtaaa ccaaccgtcg gttcgctgct ggcctcctgc tggaacgaat acattctgaa
     5341 gccggaacat actccgctgc aggattctaa cgacaacttc ctcgctaaca tccagaaaga
     5401 cggcacctac tcggtgatcc cgcgttctcc gggcggtgaa atcaccccgg aagggctgat
     5461 ggcggtaggt cgtatcgcgc gtgaatttaa tctctacacc aagatcactg gctcccagcg
     5521 tctggcgatg tttggcgcac agaaagacga tctgccggag atctggcgtc agctgattga
     5581 agccggcttc gaaaccggtc atgcctatgc gaaagcactg cgtatggcga aaacctgcgt
     5641 gggtagcacc tggtgccgct acggcgttgg cgacagcgtc ggcctcggcg tggaactgga
     5701 aaaccgctac aaaggcatcc gtacgccgca caaaatgaag ttcggtgtct ccggctgtac
     5761 ccgtgaatgt tcagaagctc agggtaaaga cgtgggtatt atcgccactg aaaaaggctg
     5821 gaacctgtat gtttgcggta acggcggcat gaaaccgcgt catgcggatc tgctggcggc
     5881 ggatatcgat cgcgaaacgc tgatcaaata tctcgaccgc ttcatgatgt tctacatccg
     5941 tactgccgac aaactgacgc gtaccgcacc gtggttagaa aacctcgaag gcggcatcga
     6001 ttacctgaaa gcagtgatca ttgacgacaa actggggctg aacgcacatc tggaagaaga
     6061 gatggcgcgc ctgcgtgaag cggtactgtg tgagtggact gaaacggtca atacgccgtc
     6121 tgcgcagact cgcttcaaac acttcatcaa cagcgacaag cgtgacccga acgtgcagat
     6181 ggtgccagag cgcgaacagc accgtccggc aacgccgtat gaacgtatcc cagtaactct
     6241 ggtggaggac aacgcatgag ccagtggaaa gacatctgca aaatcgatga catcctgcct
     6301 gaaaccggcg tctgcgcgct gttaggtgac gagcaggtcg cgattttccg cccgtatcac
     6361 agcgatcagg tgtttgcgat cagcaacatc gacccgttct tcgagtccag cgtgctgtca
     6421 cgcggactga ttgcggaaca ccagggcgag ctgtgggtcg ccagcccgct gaaaaaacag
     6481 cgttttcgct taagcgacgg cttgtgcatg gaagacgaac agttttccgt caaacattac
     6541 gaagcgcgag tgaaagacgg cgtggtgcag ctgcgcggtt aatgttttaa cgggaggcgc
     6601 aatgcctccc ctttttgcat ggtcctgtaa taatcttcgg tatattgcag gacatttttt
     6661 aaactttttg ttttattttt tgtttttatt ttttaaagga taatcaaatg tttacagaca
     6721 ctattaataa gtgtgcggct aacgctgcgc gcattgcacg cctgtcggca aataacccgc
     6781 tcggcttttg ggtcagctcc gccatggcgg gcgcgtatgt gggtcttggg atcatcctga
     6841 ttttctcgct cggtaatttg ctcgatccat ccgtacgccc tttggtgatg ggcgcgacct
     6901 ttggtatcgc tttaacgctg gtgattatcg ccggttctga actgttcacc ggacacacca
     6961 tgttcctcac ctttggggtg aaagcgggca ccatcagcca cgggcaaatg tgggcaatcc
     7021 tgccgcaaac ctggctgggt aacctggtcg gttccgtctt cgttgccatg ctctatagct
     7081 ggggtggcgg tagcctgctg ccggtagata ccagcatcgt tcactccgtc gcgctggcta
     7141 aaaccactgc accggcaatg gtactgttct tcaaaggtgc attgtgtaac tggctggttt
     7201 gtctggcaat ctggatggcg ctgcgcactg aaggggcggc gaaatttatc gctatctggt
     7261 ggtgtctgct ggcatttatc gcgtccggct acgagcactc catcgctaac atgacgctat
     7321 tcgccctctc ctggttcggc aaccacagcg aagcctacac gctggcgggt attggtcata
     7381 acctgctgtg ggtgacgctg ggtaatactt tatcgggtgc cgtattcatg ggattgggtt
     7441 attggtatgc tacgccgaaa gcgaatcgtc cggttgcgga caaatttaat caaactgaaa
     7501 cggctgccgg ttaattacta aggggttttt acgtggatca tttgcctata ttttgccaat
     7561 tacgcgatcg cgactgtctg attgtcggcg gtggtgatgt cgcggaacgc aaagcaaggt
     7621 tgctgttaga cgcaggcgct cgcttaacgg tgaatgcatt agcgtttatt ccacagttca
     7681 ccgcatgggc agatgcaggc atgttaaccc tcgtcgaagg gccatttgat gaaagccttc
     7741 tcgacacctg ctggctggcg attgcagcga cggatgatga cgcgcttaac cagcgcgtca
     7801 gcgaagccgc tgaagctcgt cgcatcttct gtaacgtggt cgatgcgccg aaagctgcca
     7861 gcttcattat gccgtcgatt attgaccgct caccgctgat ggtggcggtt tcctctggcg
     7921 gtacttctcc ggttctggcg cgcctgttgc gcgaaaaact cgaatcactg ctgccgctgc
     7981 atctgggcca ggtagcgaaa tacgccgggc agttacgcgg tcgggtaaaa caacagttcg
     8041 ccacgatggg tgaacgccgc cgcttctggg aaaaactgtt cgttaacgac cgtctggcgc
     8101 agtcgctggc gaacaacgat cagaaagcca ttactgaaac caccgaacag ttaatcaacg
     8161 aaccgctcga tcatcgtggt gaagtagtgc tggttggcgc aggtccaggc gatgctgatc
     8221 ttctgacgct gaaaggtctg caacaaattc agcaggcaga tgtggtggtc tacgaccgtc
     8281 tggtttctga cgatattatg aatctggtac gccgcgatgc tgatcgcgtt ttcgtcggca
     8341 aacgcgcggg ataccactgc gtaccgcagg aagagattaa ccagatcctg ctgcgggaag
     8401 cgcaaaaagg caaacgcgta gtgcgtctga aaggcggcga tccgtttatt tttggccgtg
     8461 gtggcgaaga gctggaaaca ctgtgtaacg cgggtattcc gttctcggtg gttccgggta
     8521 ttaccgcagc ttctggttgc tctgcctatt cgggtattcc gctcacgcat cgcgattatg
     8581 cccagagcgt gcgcttaatt accggacact taaaaaccgg tggcgagctg gactgggaaa
     8641 acctggcggc agaaaaacag acgctggtgt tctatatggg gctgaatcag gctgcgacta
     8701 ttcagcaaaa gctgattgaa cacggaatgc caggcgaaat gccggtggca attgtcgaaa
     8761 acggaacagc agtcacgcag cgcgtgattg acggtacgct cacacaactg ggcgaactgg
     8821 cgcagcaaat gaatagtcca tcgctaatta ttattggtcg ggttgttggc ctgcgcgata
     8881 aactgaactg gttctccaac cattaattta atcagggcca gagaatt
//
LOCUS       DAOFQD010000167         8867 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.167, whole genome shotgun sequence.
ACCESSION   DAOFQD010000167 DAOFQD010000000
VERSION     DAOFQD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.167"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..503)
                     /locus_tag="QBF46_004267"
     CDS             complement(<1..503)
                     /locus_tag="QBF46_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HDQ6676378.1"
                     /translation="MDFNSGGKKAIERLVEAYGFGTRQALCDHLGVSKSTMATRYMRD
                     IFPADWVIQCALETGTSLNWLTTGHGSKQASANTNTIEVEKYLLSDGALQKDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEITIRDITRLPGGRIFV
                     EGGNRAF"
     gene            649..852
                     /locus_tag="QBF46_004268"
     CDS             649..852
                     /locus_tag="QBF46_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDQ6676379.1"
                     /translation="MTPNISITLNTPHVTIERYSELTGLSIDTINDMLADGRLPRHRL
                     RKDKKREKVMINLAALTVDALSA"
     gene            917..1426
                     /locus_tag="QBF46_004269"
     CDS             917..1426
                     /locus_tag="QBF46_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HDQ6676380.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            1434..1730
                     /locus_tag="QBF46_004270"
     CDS             1434..1730
                     /locus_tag="QBF46_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HDQ6676381.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWITGEDGKRWHPCHSQDELL
                     SELTTRKRRKSKCMRQKVKWFISFVTEGRVIQYLKMICSVAIRHCRAMAVTFSR"
     gene            1848..2189
                     /locus_tag="QBF46_004271"
     CDS             1848..2189
                     /locus_tag="QBF46_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HDQ6676382.1"
                     /translation="MAINGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            2257..2490
                     /locus_tag="QBF46_004272"
     CDS             2257..2490
                     /locus_tag="QBF46_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HDQ6676383.1"
                     /translation="MRNIETLTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            2490..2717
                     /locus_tag="QBF46_004273"
     CDS             2490..2717
                     /locus_tag="QBF46_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDQ6676384.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCVECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            2714..3571
                     /locus_tag="QBF46_004274"
     CDS             2714..3571
                     /locus_tag="QBF46_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDQ6676385.1"
                     /translation="MSTILKWAGNKTAVMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PCYLVADINPDLINLYKKVAADCESFISRAKVLFEIANREVAYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLKAGDVVYCDPPYDGTFSGYHADGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKVKRSIGVAAGESKSATEIIAVSGPRCWVGFDYSRGVD
                     SSAVYGVRA"
     gene            3568..5982
                     /locus_tag="QBF46_004275"
     CDS             3568..5982
                     /locus_tag="QBF46_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDQ6676386.1"
                     /translation="MSHADMSNCCGFNEAAASFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFNESRDPVQREIEQDKLISRAKLAHEQQR
                     FNPDMVILADVSAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLNQHEKDESADGDTRTDITCMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITEYQRQQLVYELKSRGFDGSEKEVDLLLCGGSIPSGAGLRIFYRNQRLQEDD
                     KWRNMY"
     gene            6136..6324
                     /locus_tag="QBF46_004276"
     CDS             6136..6324
                     /locus_tag="QBF46_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676387.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRSHEYSRSMDVIS"
     gene            complement(6392..6691)
                     /locus_tag="QBF46_004277"
     CDS             complement(6392..6691)
                     /locus_tag="QBF46_004277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676388.1"
                     /translation="MKRITFSFTLVAIIIALAGCALPDKDGDFGAYVHACQQYAYGKS
                     YAFENRDFAYKVCKDAAKLWSDEVPGYIIRQIQLHPEIPSEEIKYAAMAGSLGNN"
     gene            complement(6800..7678)
                     /locus_tag="QBF46_004278"
     CDS             complement(6800..7678)
                     /locus_tag="QBF46_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676389.1"
                     /translation="MAMSLSCPLILLGLALFFYDWDRDMKKLTGVIAFALLLTACDKP
                     KIDASSDQSMKESIQKVRESLPADKKAQFDDAVKVVAFSQINMRELMQAGTSSGDVYE
                     TKIKSALEGKTGDEVINYAQTIRLEREKREKEQALQEIKELEAKQTSATQAAEKMKAF
                     KVERSRFYFQKEDYGNDQPILDISVENGTDKAVARVFFKGVIASPGRSVPWFSDVFNY
                     KISGGLEPGEKANWKLAPNRYSDWGKLKVPADAIFTVTVIGLEDADGKSIYGDAEFSE
                     RDADRLNQLRDKYLSK"
     gene            8314..>8867
                     /locus_tag="QBF46_004279"
     CDS             8314..>8867
                     /locus_tag="QBF46_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676390.1"
                     /translation="MTGSVPVVLSESELKSILTLTERFTWNVARPIIASLGLPTGRGR
                     EATNEKILESLIDLKSKDRQKFDGIMANVNDLILGQVVYGEKAIFSLTVDNSVIKTLN
                     DRFSFQWNLMNQPSLLVDSILDDVQLQNAVKNQPELVNYSIQNTQSILVFSSVRELVV
                     REKIPPSALAQYKQFDEIIAKRKE"
BASE COUNT         2370 a         1963 c         2162 g         2372 t
ORIGIN      
        1 tcgaaggctc tgtttccacc ctcgacgaag attcttccac cgggtaaacg agtaatgtca
       61 cggatcgtta tttcgccatc aatacttatt acccatttac catcacgtat atcatcaaat
      121 tctttatcac aaataaattc agaattgtta tctgtgatga caaaaggttt tttaaacgta
      181 gagggtagaa atcccttatc aaaaatataa aaaccgtctt tctgcaatgc tccatcagac
      241 aataaatatt tttctacttc tatagtattt gtatttgctg atgcttgctt tgaaccatgc
      301 cctgttgtta gccaattaag cgaggtgccc gtttcaaggg cgcactggat tacccaatct
      361 gctggaaaaa tatcacgcat atagcgcgtt gccatggtgc tcttagaaac acctaaatga
      421 tcacagagag cctgacgggt accgaaccca tatgcttcaa ctaaacgttc tatggctttc
      481 ttaccgccgc tattgaaatc cacaagtcct ccaaagaaat ccaaaattcg ttgacagatt
      541 ccaaaagcga tcttaaagtt gaaccagaag tgttcttttg gagccttcac tactaatcac
      601 gacaaacaac ggctcgccac aagccatacc tagaaggaat gttgccttat gacacctaac
      661 atttcaatta ctctgaatac accacatgtc acaatcgaac gttatagcga actgactggc
      721 ctttctattg atacgattaa cgacatgttg gctgatggcc gactacctcg tcatcgtctt
      781 cgtaaagaca aaaaacgtga aaaggtaatg attaacctgg ctgctctgac tgttgatgct
      841 ttgtctgctt aatagacgtc tattttcgca ataagacgct gagttcgatt ttgcgataag
      901 ttcggagttg aaaaccatgt ttgattacca agtttccaaa catccacatt ttgatgaagc
      961 ctgtcgtgca ttcgcactgc gccacaatct ggtgcaactg gcagaacgtg cgggcatgaa
     1021 tgtgcagatt ctgcggaaca agctgaaccc agctcagcct catttattaa ccgcaccaga
     1081 aatctggctg cttaccgatc tgactgaaga ttcaacgctg gtagatggtt ttctggcaca
     1141 gattcattgt ctgccatgtg taccgattaa tgaggtggca aaagagaaac tgccacatta
     1201 cgtcatgagt gcaaccgcag agatcgggcg tgttgctgca ggtgcggtat ctggcgatgt
     1261 aaaaaccagt gcaggtcgtc gtgatgctat cagcagcatt aactctgtaa cacgactgat
     1321 ggcgttggct gctgtttcat tgcaggcccg tttacaggct aaccctgcga tggcgagtgc
     1381 agttgatacc gtgactggcc tcggtgcttc attcggtttg ctgtgaggtg cttatgctga
     1441 cgaaagaacc atcatttgca tcgctgctgg taaaacaaag cccggcaatg cactacggtc
     1501 acggctggat cacgggtgag gatggaaaac gctggcatcc atgtcattca caagatgaat
     1561 tgctgtctga attgaccacg aggaaacgga gaaagtccaa atgtatgcgg cagaaagtga
     1621 agtggtttat cagtttcgtt acagagggga gagttattca gtacctgaag atgatttgct
     1681 ctgttgctat ccgtcattgt cgggcgatgg cagttacttt ttcgcgttaa aggatgggac
     1741 gtttttacgg ggagagcagg ttaaagagac gatacgaaaa aatgtatctc ctcttgagcg
     1801 ttaccgtaag aacaaagaac gatagctgcg tttgggggat atgaagtatg gcaattaatg
     1861 gcgctgcagc aactgttcca ttaagccccg gtgaacgcct gaatggactt aatcacattg
     1921 cggagttaag ggcgaaagtt tttggcctga atattgagtc agagcttgag cggtttatta
     1981 aagatatgcg tgatccacgg gatatcaata atgaacaaaa taaacgggca ctggctgcca
     2041 tattctttat ggcaaaaatt ccagctgaac gtcatagcat cagcattaat gagctgacca
     2101 ctgacgaaaa gcgggagctg attaaagcaa tgaatcattt tcgtgcagtg gtgagcttat
     2161 ttcccagacg gctaaccatg ccgaattaac caactaatga aattaatggc gtaaacccgc
     2221 cgggcatccc tttatctaaa ttcaggagaa ttgattatgc gtaatattga aaccctcacg
     2281 actaaaaccg gaccggatga cgcagggctt aatattttac tgacagaggc tcgtctggaa
     2341 gaacgccggg caagggctga agcaatggca gctcgccttg atagcctggc gtgtcatatc
     2401 tcatcccgtc agctaaacca cgtggaagcg gcagaactgc tgcgtgtgac tgctgaagca
     2461 atccagaacg aagcgcagga gatccactaa tggctgatgc aatggatctc gtacagcagc
     2521 gcgttgaaga agaacgccag cgccatatcc gtgctgcccg tgccaaaacg ccgggcgtgt
     2581 cccgcgtgct ttgcgttgag tgtgaagcgc caattccgcc agcacgccgc cgtgccattc
     2641 cgggtgtgca gctttgcatt acctgtcagg aaatcgcaga gctgaaaggc aaacattaca
     2701 acgggggggc tgtatgagca ccatcctgaa atgggcggga aataaaaccg ccgtaatgtc
     2761 cgaactgaaa aaacatcttc ctgctggtcc gcgactagtt gaacctttcg cgggttcctg
     2821 tgctgtgatg atggagacgg attatccctg ctatctggtt gcggatatta atcctgattt
     2881 aatcaacctc tataaaaagg ttgccgctga ttgtgaatcg tttatatctc gcgccaaagt
     2941 tttatttgag atcgcaaaca gggaggtggc ttattacaac ataaggcagg agtttaatta
     3001 ctcaactgaa attactgatt tcatgaaagc ggtatatttc ctgtatctca atcgtcacgg
     3061 ttaccgtggg ttatgtcgct ataacaagag cgggcatttc aacattccct acggtaatta
     3121 taaaaatccg tatttccctg aaaaagaaat tcgcgctttt gcagaaaaag cccagcgagc
     3181 aacgtttatc tgcgccagct ttgatgaaac gctggcgatg ttgaaggcgg gggatgtggt
     3241 gtattgcgat ccgccttatg acggtacgtt ttccggctat cacgctgacg gctttactga
     3301 agatgaccag tatcacctgg catccgttct tgaacatcgg tcatcagaag gacatccggt
     3361 cattgtttct aacagtgaca catccctgat ccgttcgctg tatcgcaatt ttactcacca
     3421 ctatatcaag gtaaaacgca gcatcggcgt agcagctggt gagagtaaat cagcaacaga
     3481 aatcattgct gtttccgggc cgcgctgctg ggtgggattt gattattcgc gtggcgtgga
     3541 tagttctgcc gtgtacggag tacgtgcatg agccatgccg atatgagcaa ctgctgcggc
     3601 tttaacgagg ctgccgcatc gttctcatgg aacagcccga aaaaggccat taacccttat
     3661 ctggacccgg cggaagttgc gccggtttct gcgctttcaa acctgatcac tctgtacgct
     3721 gccgataacg agcaggaaca actgcgccgc gaggcactga gtgatcaggt ctgggagcgt
     3781 tatttcttta atgaatcccg tgatcctgtc caacgcgaaa tagagcagga taagctcatt
     3841 agccgggcaa agttggcgca tgagcagcag cgttttaacc cggacatggt cattctggca
     3901 gacgtcagcg cccagccctc ccatatcagc aagccgctga tgcaacgtat tgaatacttc
     3961 agcagcttgg gcaggccaaa ggcttattcc cgctatttac gtgagacgat taagccatgt
     4021 ctggaacgac tggagcatgt acgcgacagc cagctatctg catcttttcg ttttatggca
     4081 agccatgtag ggctggacgg cctgctgatt ctgcctgaaa tgagtcagga tcaggtgaaa
     4141 cgcctgtcta cccttgtcgc tgcgcatatg agcatgtgcc ttgatgcagc ttgtggtgat
     4201 ttgtatgcca ccgatgacgt taagccagaa gaaatccgca agacatggga aaaggtggca
     4261 gcggaaaccc tgcgtctgga tgtcatcccg cctgcgtttg agcaactccg tcggaaaaga
     4321 aaccgccgta aacccgtgcc ctatgaactc attccgggtt cgctggcgcg tatgttgtgc
     4381 gccgactggt ggtaccggaa attatggaaa atgcgttgcg aatggcggga agagcagttg
     4441 cgtgctgtct gcctggtcag caaaaaagca tctccctatg tcagctatga agccgtgatg
     4501 cataaacgtg agcagcgccg caagtcactg gagtttttcc gttctcatga actggtgaac
     4561 gaagagggcg acacgctgga tatggaagac gtggtaaacg ccagcagcag caacccggcg
     4621 caccgccgca atgagatgat ggcctgtgtt aaaggtctgg agcttatcgc ggaaatgcgc
     4681 ggtgactgcg ccgttttcta caccattacc tgtccgtcac gtttccattc cacgctcaat
     4741 aacggcagac caaacccaac ctggacaaat gcgacggtaa gacaaagcag cgattatctg
     4801 gtcggcatgt ttgctgcatt tcgtaaggcg atgcacaaag ccgggttgcg ctggtatggc
     4861 gtgcgggtgg ctgagccgca tcatgacggt acagttcact ggcacctgtt gtgtttcatg
     4921 cgcaaaaaag accgccgcgc cattactgca ttgttgcgta agtttgccat ccgtgaagac
     4981 cgcgaggaac tgggtaataa cactggtcca cgctttaaat ctgagctgat aaacccgcgc
     5041 aaaggaacgc cgacaagcta catcgcgaaa tacatcagta agaacattga cgggcgtggt
     5101 ctggctggcg agatcagcaa ggaaacgggt aaatccctgc gtgataacgc tgaatacgtt
     5161 aatgcctggg cgtctctgca tcgtgttcag caattccgct tctttggtat tccgggacgt
     5221 caggcttacc gtgaacttcg cttgctggct ggtcaggcgg caaggcaaca gggtgacaaa
     5281 aaagcaggtg cgccggtact ggataacccg cgtcttgatg ccattctggc tgctgctgat
     5341 gctggttgtt ttgccaccta catcatgaag cagggcggcg tactggttcc ccgcaaatat
     5401 cacctcatca gaaccgctta tgaaatcaac gaagagccaa ccgcctatgg cgatcacggt
     5461 attcgtattt atggcatctg gtcacccatt gcagagggca agatctgcac tcatgcagtg
     5521 aagtggaaaa tggttcgtaa ggccgttgac gttcaggagg cggcagccga ccagggcgct
     5581 tgcgcccctt ggactcgtgg caataactgt ccccttgctg aaaatttgaa ccaacatgag
     5641 aaagatgaat cagcagatgg ggataccaga acggacatta cctgcatgga tgacaaggaa
     5701 ttgcacgatt acctgcacag tatgagcaaa aaagagcgcc gggaactggc agcaagattg
     5761 cgcctggtta aaccgaaacg gcgtaaagac tacaaacagc gaattacaga gtatcagcgt
     5821 cagcagctcg tctatgaact gaagtccaga ggatttgatg gcagcgagaa agaggtcgat
     5881 ttgctccttt gcggtggcag tattccgtca ggagcaggcc tgcgtatctt ctatcggaac
     5941 cagcgtttgc aggaagatga taagtggcgg aacatgtatt aatcacgcgg gttaacaatt
     6001 cgtgctctta ataataccag gcatatcagg ctgatgaacg taaaaaaacg ttttacatca
     6061 gtaagattat tatatactgt aaatataaac agtggttata tgtacagtat tgcgtatggt
     6121 gttataggag gaaagatgca ggactatttt ttggagtctt tgaagctcca gcgcattgat
     6181 ttttttctta agcttgtagc ggctagtgag tgtagtgatg aagagaaggg gctagctctg
     6241 cagtgggttt ctgaattgac tgatgagttg atggcgaaaa ttcgaagcca tgaatacagt
     6301 aggtcgatgg atgtaatcag ttgagcttgt ttcgttagga cttgataagg gaaccgaaaa
     6361 ggggcattaa gcccctttct cataagttga tttagttgtt ccccaaagag ccggccatgg
     6421 ctgcatattt aatttcttcg ctgggtattt caggatgtag ttgaatttgc ctgatgatat
     6481 agccaggaac ttcgtcactc cacagtttcg ccgcgtcctt acagacctta tacgcgaagt
     6541 ctctattctc aaaggcatag gattttccgt atgcatattg ctgacaggca tgaacataag
     6601 ctccgaagtc gccgtcttta tctggcaaag cacatccagc taaggctatg atgattgcta
     6661 ctaacgtaaa ggagaaagtt atacgtttca tggtctcttt ctaagcgttt agcattattt
     6721 aactaagggg tatctgccgt gatttggtag tccctgatat atattctcag ggactatgct
     6781 gaagtagctt taattttgat tatttagaaa ggtacttatc tctaagttga ttcagtctgt
     6841 cagcatcgcg ttctgaaaac tcggcgtcac catagatcga tttcccatct gcgtcttcaa
     6901 ggccaatgac tgtgacggta aatatagcat cagccggaac tttaagtttt ccccaatcag
     6961 agtatctatt aggggctaat ttccagttgg ctttctcacc tggttccaga cctccagaaa
     7021 ttttatagtt gaagacgtca gagaaccaag ggacgcttct tcctgggctg gcaataactc
     7081 ctttgaagaa tactctggca accgctttgt ctgttccgtt ttcaacgcta atatccagaa
     7141 ttggctgatc attgccgtaa tcctctttct ggaaataaaa acgggaacgt tcgaccttga
     7201 aggctttcat tttttccgca gcctgtgttg cagaggtttg tttggcttcc agttctttta
     7261 tttcttgcag agcctgttct ttctctctct tctcacgctc aaggcgaata gtttgtgcgt
     7321 aatttataac ctcatcacca gttttacctt ccaacgctga cttaattttt gtttcgtaga
     7381 cgtccccaga agacgttcca gcctgcatta gctctctcat gttgatttgg ctaaaggcaa
     7441 cgactttaac tgcatcatca aactgagctt ttttatctgc aggtaaggac tctcttactt
     7501 tttgaatgga ctctttcatc gactgatcgc ttgatgcatc gatcttcggt ttgtcacaag
     7561 cagtaagcag cagagcaaaa gcaattacgc cagttaattt cttcatatcc ctatcccaat
     7621 cgtaaaaaaa caaggctaat cctaacagga ttagcgggca tgacaaactc atagccatat
     7681 aggtgtgctg tataaattag cgggatgaga aagcctgcac agaccctcat gttgaaactt
     7741 gattttagct caaaagagct gacactaaat aactgcgcgg aataatgttc cactttcggt
     7801 tagcccttgg cccttggctc ttgctgggta aggcgtgggg ccgttttctt cgcctgatcc
     7861 tgctgtatct ggcctacagt atgttgtcct tacgcgacat gccacatgtc agtaaacggc
     7921 aggtgtagca gtattagacg cagctattta gtgtgtatta agctggctca tatttatgac
     7981 tggtttcaag gtttgaccga ttttcatgtc atgcatgtct atgctgcatg agttcgcatg
     8041 atcgtttgag gatcggtttg gctaagacat gccagaactg gcgggctttt gctcatgtca
     8101 tgcaggtgca tgaaaactac tacataaagc gggcaggcgt ggcggggata cgagcgcgcg
     8161 ctcgcttagt tttgtaacac tctgagtaag gtcgcttttt agaagcaaat ggcttgcaaa
     8221 ttttttcaac attgaggtaa tttgttaggt atcgaaggat aaattaagac aagagcaaat
     8281 tcttattgct ttttggatga ctaggtgcaa gttatgacag gtagtgtccc agtagtgctt
     8341 tctgagtctg agttaaagtc gatcttgacc ttaactgaac gtttcacatg gaacgttgca
     8401 aggccaatta ttgcgtcttt agggctgcca acaggtaggg ggcgtgaagc aacaaacgaa
     8461 aaaatactag aaagcctcat tgatcttaaa agtaaagata ggcaaaagtt tgatggcatt
     8521 atggcaaacg taaatgacct aattttgggt caggtcgttt atggtgaaaa agcaatattt
     8581 agtttgactg tagataatag tgtaattaaa acactaaatg acagatttag tttccaatgg
     8641 aatttgatga atcaaccatc attattggtt gattcgatcc ttgatgatgt ccaattgcaa
     8701 aatgctgtaa aaaatcaacc tgaacttgtt aattattcta ttcaaaacac ccagtcaata
     8761 cttgtttttt catctgtcag agaattagtt gtaagagaaa aaattccccc gtcagcactt
     8821 gcgcaataca agcagtttga tgaaattatt gcaaagcgaa aagaaaa
//
LOCUS       DAOFQD010000168         8824 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.168, whole genome shotgun sequence.
ACCESSION   DAOFQD010000168 DAOFQD010000000
VERSION     DAOFQD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.168"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..731
                     /locus_tag="QBF46_004280"
     CDS             <1..731
                     /locus_tag="QBF46_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HDQ6676391.1"
                     /translation="TLPDNAMILKSVLAVKLVMQLKILNIVNKNFIENMKKIFSHCPY
                     IKDPIIRSYIHSDEDNKFDDFMRQHRFSEVNFDTQQMIDFINRFNTNKWLIDKNNNFF
                     IQLIDQALRSTDDMIKANVWHLYKEWIRSDDVSPIFIETEDNLRTFNTNELTRNDNIF
                     ILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTQETLFSSKS
                     HGKYALFPIFTASWRAHRIMNKGV"
     gene            954..1451
                     /gene="ubiC"
                     /locus_tag="QBF46_004281"
     CDS             954..1451
                     /gene="ubiC"
                     /locus_tag="QBF46_004281"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDQ6676392.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1464..2336
                     /gene="ubiA"
                     /locus_tag="QBF46_004282"
     CDS             1464..2336
                     /gene="ubiA"
                     /locus_tag="QBF46_004282"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDQ6676393.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2491..4914)
                     /gene="plsB"
                     /locus_tag="QBF46_004283"
     CDS             complement(2491..4914)
                     /gene="plsB"
                     /locus_tag="QBF46_004283"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDQ6676394.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEQVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5085..5453
                     /gene="dgkA"
                     /locus_tag="QBF46_004284"
     CDS             5085..5453
                     /gene="dgkA"
                     /locus_tag="QBF46_004284"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDQ6676395.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5563..6171
                     /gene="lexA"
                     /locus_tag="QBF46_004285"
     CDS             5563..6171
                     /gene="lexA"
                     /locus_tag="QBF46_004285"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDQ6676396.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6244..7569
                     /gene="dinF"
                     /locus_tag="QBF46_004286"
     CDS             6244..7569
                     /gene="dinF"
                     /locus_tag="QBF46_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDQ6676397.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPVVGVWCYLLDGMFIGATRATEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7685..7894
                     /locus_tag="QBF46_004287"
     CDS             7685..7894
                     /locus_tag="QBF46_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDQ6676398.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7936..8451)
                     /gene="zur"
                     /locus_tag="QBF46_004288"
     CDS             complement(7936..8451)
                     /gene="zur"
                     /locus_tag="QBF46_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDQ6676399.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2087 a         2105 c         2339 g         2293 t
ORIGIN      
        1 atacattgcc agataatgca atgatattga aatccgttct ggccgtaaaa ctggtgatgc
       61 aattaaaaat acttaatatt gttaataaaa actttattga gaatatgaag aaaatattta
      121 gccattgtcc ttatataaaa gatcccatta tacgcagtta tatccattct gatgaagata
      181 acaagttcga tgattttatg cgtcaacatc gattcagtga ggtgaatttc gatacccaac
      241 agatgatcga ttttattaac agatttaata cgaataaatg gctaattgat aaaaataaca
      301 atttttttat ccaacttatc gatcaggcct tacgatcaac ggatgatatg atcaaagcaa
      361 atgtttggca tctttataaa gagtggattc gtagtgatga tgtttcacct atatttatag
      421 aaactgaaga taatttaaga acctttaaca cgaatgaatt aacacgaaac gataatatct
      481 ttatcctgtt ctcctcagtc gatgatgggc cagttatggt ggtaagctcc cagcgcttac
      541 atgatatgtt gaatcctaca aaagatacca attggaattc cacgtatatc tacaaatcca
      601 gacatgagat gttgcctgtt aatcttactc aggaaacact tttcagctcc aaatctcatg
      661 gtaaatatgc gcttttccca atttttactg cgagttggcg agctcatcgt ataatgaata
      721 agggtgttta agtaaaggaa aacatcaccg ttcctggcat cctggacggt gatgccccta
      781 cggttgccct cgccagcacg ggcatcggta aagcgtaagg ttcaacatcg ttttaccact
      841 tcatgcgatt gttgcgtttt tgttgcgtat tagatcactt aatttgcttt acatctcccg
      901 taaacacttt tctgcgatac aatgccttta cgttatgtaa cggagagttc ggcatgtcac
      961 accccgcgtt aacgcaactg cgtgcgctgc gctattttaa agagatccct gccctggatc
     1021 cgcaactgct cgactggctg ttgctggagg attccatgac aaaacgtttt gaacagcagg
     1081 gaaaaacggt aagcgtgacg atgatccgcg aagggtttgt cgagcagaat gaaatccccg
     1141 aagaactgcc gctgctgccg aaagagtctc gttactggtt acgtgaaatt ttgttatgtg
     1201 ccgatggtga accgtggctt gccggtcgta ccgtcgttcc tgtgtcaacg ttaagcgggc
     1261 cggagctggc gttacaaaaa ttgggtaaaa cgccgttagg acgctatctg ttcacatcat
     1321 cgacattaac ccgggacttt attgagatag gccgtgatgc cgggctgtgg gggcgacgtt
     1381 cccgcctgcg attaagcggt aaaccgctgt tgctaacaga actgttttta ccggcgtcac
     1441 cgttgtacta agaggaaaaa aatatggagt ggagtctgac gcagaataag ctgctggcgt
     1501 ttcatcgctt aatgcgtacg gataagccaa ttggcgcgtt actgctgctc tggccaacat
     1561 tatgggcgtt gtgggtggcg acaccgggcg ttccccagct ctggatcctg gcggtgtttg
     1621 tcgcgggtgt ctggctgatg cgcgctgccg gatgtgtggt gaatgattat gctgaccgca
     1681 agtttgatgg tcatgttaag cgcacggcga accgaccact tcccagcggc gcggtaacag
     1741 agaaagaggc gcgcgcgctg tttgtcgtgc tggtactgat ttcgttttta ctggtgctga
     1801 cgctgaatac gatgaccatt ctgttgtcga ttgccgcgct agcgctggcg tgggtgtacc
     1861 cgtttatgaa gcggtatacc catctaccgc aagtggtgct gggcgcggcg tttggctggt
     1921 cgattccaat ggcttttgcc gctgtgagtg agtcggtgcc attgagttgc tggttaatgt
     1981 tcctcgccaa tattctctgg gcggtggctt acgacacgca gtatgcgatg gttgaccgcg
     2041 atgatgatgt gaagattggc attaaatcca cggcaatcct gttcggccaa tacgataaat
     2101 tgattattgg tattttgcag attggcgtac tggcactgat ggcgatcatc ggtgagttaa
     2161 atggcttagg ctggggatat tactggtcaa ttctggtggc tggcgcgctg tttgtttatc
     2221 aacaaaaact gattgccaac cgcgagcgtg aagcctgctt taaagcattt atgaataata
     2281 actatgttgg tctggtactg tttttagggc tggcaatgag ttactggcat ttctgatgat
     2341 gtaaaaaagc cggatgatca tccggctttc ttctgggttg cctgatgcgc tgcgcttctc
     2401 aggcctacac aacagatcgc aatttattga atttgcagat catggtaagc cggataaggc
     2461 gttttcgccg catccggcaa ttctctctga ttacccttcg ccctgcgtcg cactctcaat
     2521 cgtcaaacgc acgtctgatg taatcaactc cgccagtaac tgataaacct tcatcgtttc
     2581 tgccggttcg gcgtcgccgc tatcgctgat atacccttca tcacgcagcg tcagcaccag
     2641 agaactgaac accgccttgt cgaagaactc cggcgcgttg atgccgtgca gcacggagag
     2701 acgttgcgcg acagtgcggc tctctttctc cagcgtaccg cggttaatcg acgggttggc
     2761 gctcaacagc cagaaggtaa tggcataacg ttgcagcgtt tcgcgcgcgc ctgcggccag
     2821 cagctgtagt gtgcgagaat gcgccgggtt gatatgcaac tcatcatctt gcagggtaat
     2881 cagcccctga cgttgcatct catttgccag cgcatcgata acgtccggca actcgtcgcg
     2941 atcccagcgc aggaacagct ccgctttcag cattgggtaa agcacattga cgtgctccat
     3001 caatacgtcg cgggagatgt ggcgatgctg ggtgacaatt gccgccatca gcgaaggcag
     3061 caccaacata tgcgcaatgt tgttgcgata gtaggtcatc aacaccgctt gctcgcgcgg
     3121 cagaatgatg atgtcgccga tagtgtcttt ctcgacttca aacttgttca tttgcagcgc
     3181 gtgatcgata agctcgctgg cgctggctga aggaacggta gagtccgtgg agtagggcac
     3241 gttgcgcatc agatccaggt agcagttgag ttgctcggtt aactgctcgc gggtgagtga
     3301 gcgctgacgt gatgccagta gcgcagtaca gcacaggttc atggcgtttg ccgcgcctgc
     3361 gttgttaatg cgtaccatca gatcggcagc aatattattg accgtcggcg ttaaccatgc
     3421 cggacgcacc gcttcgatgg gatcgataga ttcacgccag tcaggtacat gctggttaag
     3481 gtaggtcatc aacggcattg gttcaccgaa gttgacgtaa ccctgaccga gattacgcag
     3541 cttgcttaaa ccgcgcagca tctgcggcag gctctctttc tctttcgtcg cgccgcgcag
     3601 ttctttggcg taagtaccca cttccatgac ttgctcataa ccgatataga tcggaatcag
     3661 cgtaatcgga cgcgtgccgc cacgcagcat cgcctgaatg gtcatcgaca gcgtaccagt
     3721 tttcggatcc agcaaacgcc ccgtacggga acgaccgcct tccacgaagt actcgacgga
     3781 ataaccacgg ctgaacagtt cgccgagata ctcccggaaa acggtggaat aaagtttatt
     3841 gcctttaaac gtacggcgaa taaagaacgc ccccagacgg cggaaaatcg gcccggcagg
     3901 ccagaaattc aggttgatcc cggcggcgat atgcggcggc accagcccct ggtgatacag
     3961 cacgtaagaa agcagcaggt agtccatgtg actgcggtgg caaggcacat ataccagctc
     4021 atgaccgtcg tgggccagct ggcgaacgcg ctcagcgtta tggacgttga tgccctggta
     4081 aagtcggttc caggtgaagc ccagaatacg gtcagtcagg cgaatcatct cgtaagagaa
     4141 attcgccgca atctcttcca tcagtgcaat cgcgttctgc tgcgcttttt catgggagat
     4201 ttttttgctg cgcgcttcat cttctaccgc tttggcaatg gcgcgggagg cgagcagctt
     4261 attaaacaga tcctgacgag caggcaaacg tgggcctacg gcagccagac gttgacgggc
     4321 aaagtgcata cgcgccacgc gcgccagttt ctgagcgata gttttatccg tgccgtgttc
     4381 atccgccata cggcgcagcg aaactgacgg cgagaaacgc acaaaactgt cgcgaccgag
     4441 ccacagtaca gcgaaaaatt tctgtacgcc gttaagcata cgcagcggcg ggttcacttc
     4501 gcctttttca cgccccggcg cgcgaccaaa catcaccgac actggcacca tctgcacatc
     4561 cagatttggg ttgctacggt gcaaatcgag atagtcgtgg aacagcttaa tagactcttc
     4621 tttcggcgtg taataggtga acacacgcgg cccgccgtga atgaacacat agcgcggcag
     4681 tagtgtgccg tcgatttcca gcggctctaa cgggtcaggc aagtcatgtg ccagacactg
     4741 ggcgcgcaac gtcagcaaat ctgctttcga gttgtacggt aaaacgtaca taattggacg
     4801 agaggtatcc agccccagtt ccggggcagg atctgccgga atagacttgc tttttaccag
     4861 gatgcttaat ggtaaattca gtaatttgta gtaaattcgt ggccagccgg acataaacga
     4921 tgtaaagcct ctggttaata atgcaaatgc gcggcaagga tagcagaaag tcatgggaaa
     4981 ttctgtggta tccgctcatg tttcgcgcgg cgctacgcaa acccgaatca tcggatttaa
     5041 cggtacactg atattgacgc tcataatgta aaaaggttct ttcaatggcc aataatacca
     5101 ctggattcac ccgaattatc aaagctgctg gctattcctg gaaaggttta cgcgctgcat
     5161 ggatcaacga agcggcattc cgtcaggaag gcgtagcggt attgttggcg gtggtcatcg
     5221 cctgctggct ggatgtggac gcgattaccc gcgtgctgct tatcagctcc gtgatgctgg
     5281 tgatgattgt ggaaatcctc aatagcgcca tcgaagcagt ggttgaccga attggctctg
     5341 aataccatga gctttccgga cgcgcaaaag atatgggatc cgctgcggtg ctgattgcca
     5401 ttatcgtcgc cgtgattacc tggtgcattc tgttatggtc gcattttgga taacccttcc
     5461 agaattcgat aaatctctgg tttattgtgc agtttatggt tccaaaatcg ccttttgctg
     5521 tatatactca cagcataact gtatatacac ccagggggcg gaatgaaagc gttaacggcc
     5581 aggcaacaag aggtgtttga tctcatccgt gatcacatca gccagacagg tatgccgccg
     5641 acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc caaacgcggc tgaagaacat
     5701 ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt ccggcgcatc acgcgggatt
     5761 cgtctgttgc aggaagagga agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa
     5821 ccacttctgg cgcaacagca tattgaaggt cattatcagg tcgatccttc cttattcaag
     5881 ccgaatgctg atttcctgct gcgcgtcagc gggatgtcga tgaaagatat cggcattatg
     5941 gatggtgact tgctggcagt gcataaaact caggatgtac gtaacggtca ggtcgttgtc
     6001 gcacgtattg atgacgaagt taccgttaag cgcctgaaaa aacagggcaa taaagtcgaa
     6061 ctgttgccag aaaatagcga gtttaaacca attgtcgttg accttcgtca gcagagcttc
     6121 accattgaag ggctggcggt tggggttatt cgcaacggcg actggctgta acatatctct
     6181 gagaccgcga tgccgcctgg cgtcgcggtt tgtttttcat ctctcttcat caggcttgtc
     6241 tgcatggcat tcctcacttc atctgataaa gcactctggc atctcgcctt acccatgatt
     6301 ttctccaata tcaccgttcc gttgctggga ctggtcgata cggcggtaat tggtcatctt
     6361 gatagcccgg tttatttggg cggcgtggcg gttggtgcaa cggcgaccag ctttctcttt
     6421 atgctgttgc tgtttttacg catgagcacc accgggctga ctgcgcaggc ttatggtgcc
     6481 aaaaatcctc aggcattagc ccgtgcgctg gtgcaaccgt tgctgttggc gttgggggct
     6541 ggggcgttaa ttgcgctgct gcgtacgccg attatcgatc tggcgctgca tattgttggc
     6601 ggtagtgagg cagtcctgga acaggcgcgg cgctttcttg aaatccgctg gttaagcgca
     6661 ccggcgtcgc tggcgaatct ggtattactc ggttggttac tcggcgtgca atatgcccgt
     6721 gcgccagtaa ttttgttagt ggtcggcaat atcctcaaca ttgtgctgga tgtctggctg
     6781 gtgatggggc tgcatatgaa cgtgcagggc gcggcgctgg cgacggttat tgcggaatat
     6841 gcaacattgc tgattggtct gctaatggtg cgtaaaatcc tcaaactacg cggaatctcc
     6901 ggcgaaatgc tgaaaactgc ctggcgagga aacttccgtc gcttgctggc gcttaaccgc
     6961 gatatcatgc tgcgttcgct gttgttgcaa ctctgtttcg gcgcgatcac cgtacttggc
     7021 gcgcgactgg ggagtgacat tatcgctgtt aacgcggttc tgatgacgct actcaccttt
     7081 accgcctatg cgctggatgg ttttgcctac gcggttgaag cgcactccgg tcaggcatac
     7141 ggtgcgcgcg acggtagcca gttgctggat gtctggcggg cagcgtgccg ccagtcgggg
     7201 atcgtagcgt tactgttttc ggtggtttat ttgctggctg gggaacacat cattgcgttg
     7261 ctgacgtcgt taacccagat tcagcagctg gctgaccgct atcttatctg gcaggtgatt
     7321 ttgccggtgg ttggcgtctg gtgttatctg ctggacggca tgtttatagg cgcaacgcgt
     7381 gccaccgaaa tgcgtaacag tatggcggtg gccgccgcag gttttgcgct gacgctcctt
     7441 acgctgccgt ggctgggtaa tcatgctttg tggctggcat taaccgtctt tctggcgttg
     7501 cgcgggcttt ctctggcggc tatctggcgg cgtcactggc gcaatggtac ctggtttgcc
     7561 gcaacgtgac ggttaaaaat tctgaataaa taatcctaag ccaaattgct gactacactt
     7621 aatctcacgt tcagaagaaa agtgaacgta ctctcattca caacctaacg atgaggtctt
     7681 gattatgaat aaagatgaag ccggcggtaa ctggaaacag tttaaaggta aagtgaaaga
     7741 gcaatggggc aaactgaccg atgatgatat gacgatcatt gaaggtaaac gtgatcaact
     7801 ggtcggtaaa atccaggaac gttatggtta tcagaaagat caggcagaaa aagaggtcgt
     7861 ggattgggaa acccgcaatg aatatcgctg gtaattaatc cctcctgccc gacgtgtaca
     7921 aggatgtacg ccctcttaac gcggtttctt tttcacctgc acagagtgat catgctggca
     7981 ctgttcagga tgacgacacg cttccacttc cacgcatgcc gcacagagcc catgtgcttc
     8041 aatcacatta tgccgcaggg caaaccccat ttttgccgcc agcgtatgca taatgtcttc
     8101 cacgccttct gcacactctt ctttcaccgc gccgcagcga tcgcaaataa acatggctga
     8161 cgtatgcgtg ggctggtcga acagatgaca gagcacataa ctgttggtgg attccacctt
     8221 atgcacaaaa ccttgctcaa gcagaaaatc cagcgcgcga taaaccgttg gcggcttggc
     8281 ttgcggttca gcttcgcgca gtaaatcaag cagatcataa gcgctgatag cgccatcctg
     8341 cagactcatc aggcgcaaca cttccaggcg ctgtggggtc aggcgcacat tacgctgcgc
     8401 gcagattttt tcagcctgcg ctaataactc ctgcgttgtg gtcttttcca tcgggcacct
     8461 caaagtgggg gttaagaatg tccttacttt accatgttcc aggaaaaaca ccgagaaccc
     8521 ttattgttgc cgtaatgttg attttctgtt ttgtaggtaa ggtgttgtgt tgccttgtcg
     8581 taccattatc aacacgataa taattaataa ttcattttta aataaattgc ttcaagttaa
     8641 ctcgctattt atttaactgg gtttaaacgc atacctgtag ttcagaaatt atgagcgatt
     8701 tttgatagtg accagatggc aaaagaatta taaggaggac acggtacgac aaaaaaggta
     8761 gatgaatcat gttgaagata atacccggtg caactggata ctttaataaa acactcaatt
     8821 caaa
//
LOCUS       DAOFQD010000169         8673 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.169, whole genome shotgun sequence.
ACCESSION   DAOFQD010000169 DAOFQD010000000
VERSION     DAOFQD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.169"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(234..425)
                     /locus_tag="QBF46_004289"
     CDS             complement(234..425)
                     /locus_tag="QBF46_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDQ6676400.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            443..595
                     /locus_tag="QBF46_004290"
     CDS             443..595
                     /locus_tag="QBF46_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676401.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            777..3020
                     /gene="pdeF"
                     /locus_tag="QBF46_004291"
     CDS             777..3020
                     /gene="pdeF"
                     /locus_tag="QBF46_004291"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDQ6676402.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAGYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(3059..4600)
                     /gene="ppx"
                     /locus_tag="QBF46_004292"
     CDS             complement(3059..4600)
                     /gene="ppx"
                     /locus_tag="QBF46_004292"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDQ6676403.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDGSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4605..6671)
                     /gene="ppk1"
                     /locus_tag="QBF46_004293"
     CDS             complement(4605..6671)
                     /gene="ppk1"
                     /locus_tag="QBF46_004293"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDQ6676404.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6842..7480)
                     /gene="purN"
                     /locus_tag="QBF46_004294"
     CDS             complement(6842..7480)
                     /gene="purN"
                     /locus_tag="QBF46_004294"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDQ6676405.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7480..8517)
                     /gene="purM"
                     /locus_tag="QBF46_004295"
     CDS             complement(7480..8517)
                     /gene="purM"
                     /locus_tag="QBF46_004295"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDQ6676406.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
BASE COUNT         2174 a         2107 c         2106 g         2286 t
ORIGIN      
        1 agggatagat taaatttatt aaaattcacg cagggaataa tttataaaaa tatatcatta
       61 caaaatatat attaatttta tatttatttc gcagccaaac aggataaaca tcagtcagga
      121 caaagacgta aaatttaaga tgaaagaata tctgccgctt tgataattcc aaagagcaaa
      181 agcatccgac agcgaagtgc cgttatgttc tgtgaaagtg gagaaggtaa ccgctaacgt
      241 tgtaccggtg attcgacgga gagtgtggat tgctgagctt cttttttgct ctgatggtgc
      301 tgccaggcac cgacggacga gtagataaaa cggccaaaga agaagataaa gctgatgagc
      361 agtacgatac gggtcatgcg actgttaaat cggtgtcgtt ttcgcatact ggttgcctga
      421 ctcacaaaag gttccttgaa gtatgtccca cgccgtggac ggtacttaca ttaaggcaca
      481 acaggacaaa atggtcaatt cttcgttatg taaaaaagcg tcagtggata catattttaa
      541 tgttatggaa gttaatttaa ttatttacaa tgatgatgta ataatgatga attaacatga
      601 taaacgtaat aattcattaa tcagggttat tttgtttgat atatatcaat tggtactttc
      661 atatgacact tagaatcaat gctctctata gtgatgaata atcatcattc gaagtcaggt
      721 gggatgcctg tctgaataca cctccttcag gatgtggggg attctgctga gcatctatga
      781 aactgaatgc aacttatata aaaatacgtg ataaatggtg ggggcttccg ctgttcctgc
      841 cttctttaat cttgcccatt ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg
      901 aggtttttct cttttatctg cctctggcac tgatgatcag catgatgatg tttttcagct
      961 gggcggcatt gccagggatc gccttaggga tttttgtccg caaatatgca gagctgggtt
     1021 tttacgaaac gctatcatta acggctaatt ttattatcat tatcattctc tgttggggcg
     1081 gttacagggt ctttactccc cggcgtaaca acgtttcaca tggtgatacc cgtttaattt
     1141 cccagcgtat attctggcag attgtgtttc ctgcaacgct gtttctgata cttttccagt
     1201 ttgctgcatt tgtaggatta ctggcgagca gagaaaatct ggtcggtgtc atgcctttta
     1261 acctcgggac tttaatcaat tatcaggcct tactggtggg taatctgatc ggtgtcccgc
     1321 tgtgctactt catcattcgg gtagtgcgaa atccatttta tttacgtagc tattattcgc
     1381 aattaaaaca gcaggttgat gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg
     1441 cattaggtgc tttattgttg ctgttatgca tgccgttaaa tgaaaaaagc acaattttta
     1501 gcaccaatta taccttgtca ttattgctgc ccctgatgat gtggggagcg atgcgctatg
     1561 gttataagct gatttcgctg ctctgggcgg tcgtgttgat gatcagcatc cacagctatc
     1621 aaaattacat tcccatttat cctggctata ccacgcagct gaccataacc tcctccagtt
     1681 atctggtatt ctcttttatt gtcaattata tggctgtact ggcaacccgt cagagagcgg
     1741 tagtcagacg cattcagcgg cttgcgtatg tggacccggt ggttcatctg ccaaatgttc
     1801 gcgccctgaa tcgcgcgttg cgtgatgccc cctggtctgc gctttgttat ttacgcatcc
     1861 ctggcatgga aatgctggtt aagaactatg gcatcatgct gcggattcaa tacaagcaaa
     1921 aactttctca ctggctgtca cccttgctgg aaccgggtga agatgtttat cagctttcgg
     1981 gtaacgatct cgcgctgcga ctgaatacag aatcgcacca ggagcgcatt accgcactgg
     2041 atagccatct caagcaattt cgtttctttt gggatggcat gccgatgcaa ccgcagattg
     2101 gcgtcagtta ctgctatgtg cgctcgccag tgaatcatat ctacctgctg ctgggagagc
     2161 taaatacggt cgccgaactt tccatcgtga ccaacgcccc ggaaaatatg cagcgtcgcg
     2221 gggcaatgta tttgcaacgc gaattgaaag ataaagtcgc gatgatgaat cgactacagc
     2281 aggcgctgga acacaaccat tttttcctga tggcccagcc gattaccggt atgcgtggtg
     2341 atgtttacca tgaaattctt ctgcgcatga aaggtgagaa tgatgaactg atcggccccg
     2401 atagcttctt gccggtcgcg cacgaatttg gtttatcgtc gagtatcgac atgtgggtca
     2461 ttgagcatac gctgcaattt atggctgaaa acagagcgaa gatgcccgct caccgttttg
     2521 ctattaatct gtctccaacc tcggtatgtc aggctcgttt tcctgttgaa gtcagtcagt
     2581 tgctggctaa atatcagatt gaagcgtggc aacttatttt tgaagtcacc gaaagtaatg
     2641 ctctgaccaa tgttaagcag gcgcaaatca ccttgcagca tcttcaggaa ttaggctgcc
     2701 agattgcgat tgatgatttc ggcaccggct acgccagcta tgcacggctt aaaaatgtga
     2761 atgccgatct gcttaaaatt gacggcagtt ttatccgcaa tattgtgtca aatagtctgg
     2821 attatcagat agtggcatcg atttgccacc tggcgcgaat gaagaaaatg cgggtagtgg
     2881 cagggtacgt tgaaaacgaa gagatccgcg aggcggtgct ctctttgggg atcgattata
     2941 tgcagggtta tcttattggt aagccgcagc cgttaattga tacgttgaat gaaatcgaac
     3001 ccattcgcga aagtgcctga ataatgcggg ccgacatttc tcgtcggccc gcaaagcatt
     3061 aagcggcgat ttcaggtgta ctttcttctt caattttcaa ccgccagcca gccacgcctt
     3121 cccagtattc ttgctccttt tccagatcaa gcagtaccaa cgcattctga ctaaaccagt
     3181 catgcgggaa acgcagtgtc cagtgactgc catcagtaat cagtgtcaat gttggcggtg
     3241 tggtggttgc ctgacgttga ttgttgagta atacgccaag gcgcaatagc tgaatcagtg
     3301 gcaggaactg tttcttctta aacaaggtaa agcgcggcag atcgtcgagt ttaatcgctt
     3361 tacggtgata gcgcaccagt gtcgccatca tcagctgctg ttcctgatta aaacccggca
     3421 agtcactgtt ttgcagaata taagcggagt ggcgatgcaa accgctgtgg ttgatattca
     3481 acccgacctc atgcagcatg gcggcccatc gcagtagcgc ctccagttgc ggatgcgcca
     3541 gcttcggttg ctgttcccgc cactgttcgt acatttgcat agtggtatcc agcacccgtc
     3601 gggcctgttc gctgtcgatg tgatactggt tggcgaggct gctggcggtg cgactacgca
     3661 catcctgatg acggaaacgt ccttccattt cgtacagtac gccttcgcgt aacgccccgt
     3721 cagaaaggcg cagttcacgg atggctaaag catcaaacac accgcataaa atcgccagtc
     3781 caggaacgaa gactgttttc cgctcttcgg aaagacccgg taaactcagc gatgcgaaat
     3841 tacggtgacg taaaacttct tttaccagtt tttccagacg ttccggggta attatcccgt
     3901 ctttctcgcc catttccatt aacacttcat gggcggcttt tatggtaccg gaagcgccca
     3961 ttgccacgtt ccagccctga atacggaatt gccaggttaa agtttccagt ttttgtgctg
     4021 ccgccatacg agcgcgctga aaattctctt tattgatgac cccgccaggg aagtaaagct
     4081 gggcaaagct gacacaaccc atccggcggc tttcaacgag aatcggttcg aaattttcac
     4141 caatcaccag ttccgtagat ccgccgccaa tatcaataac cagtttgcga cctttctccg
     4201 gttgggtatg ttccacgccc ataaaaatca gacgggcttc ttcattaccg gaaataattt
     4261 caatcgggta gggaatgacc ttttccgcgc gtttcagaaa gtcagtggcg ttcagcgcct
     4321 gacgcagcgt atgggtgccg actatacaga cgctggcagg agaaaaccct tgtagccgtt
     4381 cggcaaacag cgacagacag ttcaaaccgc gcgtcattgc ctcttcactc aacatattat
     4441 ctggccccag gccgtccgcc agatgcaccc gctgtttcag gcgaccaata atctgcatgg
     4501 caccatctac cacacgggct atgaccatgt gaaaactgtt tgaaccaaga tcgaccgcag
     4561 caaactcctg cggacgaggg gatttatcgt gtattggcat agggttattc aggttgttcg
     4621 agtgatttga tgtagtcgta aatcgccaac tgcgcccgta ctttgcggcg attgccgcgg
     4681 ggaacgtagc gattactgag ttctttatcg atataacgtg ctttgaccgt atcgctgaac
     4741 aatatgtcga tgatgtccag cacccgctgc ttcaggcgcg gatcgagcag cggtgtcgcc
     4801 acttcaatac gataatcaat attgcgcgtc atccagtcgg cggaagaaag gtagaccttt
     4861 ttatcgccgc cattttcaaa aatataaacc cggtcatgtt caaggtaacg gtcaacaata
     4921 ctgatggcac gaatgttgtc gctaatgcct tccagattgg ggatcagcga acacattccg
     4981 cgaaccagca gattaaccgg tacgccggag ctggaggccg catacagacg atcaaccagg
     5041 cctttatcga caaggttatt tagcttcagg gtgataccac tgggcagccc ttgctgcgcg
     5101 ttggcgattt cgcggtctac catttcatat aacagacggc gagagttttg cggtgacacc
     5161 attaaataat caaatgtcac cgggcggtat gggttttcaa taaagttaaa tacccgccgt
     5221 acttcgttgg tgatacgcgc atctgcggtc agcaacgaat agtcagtata aagacgcgcg
     5281 gttttttcgt taaagttccc ggtcccgatg tgtgcgtaac ggaccacttc accgttttct
     5341 ttacgtgaaa tcaggaacag tttggcgtga attttcagcc ccggcgcaga gaagataacg
     5401 tgcacgcctg cttctgtcag gcgcttcgcc cagtgaatgt tggcttcttc gtcgaaacgc
     5461 gcctgtaact caaccaccac ggtcactttc ttgccgttgt gcgcagcgtg gatcatcgag
     5521 tcgatgatac gtgaatcttt tgccacacgg taaatgttga ttttaatcgc cagcacgctc
     5581 ggatcgaacg aggcctgacg cagcagttcc agcacatgct caaaggtgtg ataaggataa
     5641 tagagcaaca catcgcgttc gcgaatggca tcaaaaccat tgcggaactg ggctttatca
     5701 aaccaaatgt ggcgtaaacg cggcagtggt ttgttcacca gattggcttt gccgacattg
     5761 gggaaattaa taaagtcttt aaaattatga taacgaccgc cggggacgat ggagtcgtag
     5821 cgggaaatag tcagtttttc gcgtaacact tcaaccagcg cattgggcat atcgcgctga
     5881 taaacaaaac gcaccggctc agcagttaaa cgctgcttga gactggaaga catcaactcc
     5941 atcaggctgg cttccatctc atgcactaaa tcgtattcgg catcgcgggt catcttcatt
     6001 gaataggcat tcagcgcgtc ataatcaaag aagcctttga aaatatcatc gaggcagtaa
     6061 cgcagaatgt tatccagaag aatcatcggt ttgcgtcgac gcggcgcttc tggcggtaaa
     6121 ttcacaaagc gtggcacttt atctgatggg atctccagca gcgcgtaacg gatggtatcg
     6181 ccacggataa tttccaccgc caaataggtg taatcatctt tcaggaactg cactaagtca
     6241 gtgtcaggat tgattaaaat cggcgtaatg tgctgacgca gatactgctt aaaataatga
     6301 cgaagccagt tttgttgatt gacggagagc tggcgttcat taatcaggaa gatctggtta
     6361 cgcgccatct ccagcagcag ctcgttgtag aggccgtcga attcctgatc ggctttcagc
     6421 acccgggact gaattttgcc cagtaaatgg cgggaatgag agttggagcc ttgttcttcg
     6481 ctaataatga tgcgtcgctt cagttcagcg aagcggactt tatagaactc atcaaggtta
     6541 ttggaataga tccccaggaa acgcatcctt tcaatcagcg ggttagattt gtccgccgct
     6601 tcctgaagca cgcgttcatt gaacgataac caactgagct ctttttcgat gtatagcttt
     6661 tcctgaccca ttaccacttt tactccgttt tattcacggg acactgcctg gatattatgg
     6721 cgagcaatat tggcagatgt ccaactatgc cagaaaaggg ggaaaaaaac tgctggagct
     6781 tgcgatttac aggtgaattg aaaaacgcca gcggcagagc tggcgcttta actacggggc
     6841 attactcgtc ggcagcgtag ccctgcggcg gcagacgttg accatccagc cacgcggcgt
     6901 tttcgtgcat tttcagacga ccatcggcaa accagctaat caccagtgga taaatagcgt
     6961 gttcctgggt ttgcacgcgg gcggtgatgt catcttccgt atcaccagca aataccggga
     7021 ctttcgcctg taaaataacc gggccaccgt ccagttcatc ggtgacgaaa tgcaccgatg
     7081 taccgtgctc ttcatcgcca ttttccagcg cctgacgatg ggtgtgtaat ccgggatatt
     7141 tcggcagcag agaagggtga atgttcagca aacgcccggc atagtgggag acaaacgccg
     7201 ggctgagaat gcgcataaaa ccagccagca cgaccacatc gggtgcgtac atgtcgattt
     7261 catgaatcaa ctcccggtca taggcttcac gactgtcaaa cgcgctggcg atgagcgtat
     7321 gcgttgcaat acccgcctgg cgggcgcgtt caaggccgaa cgcgtcggcc ttattgctga
     7381 aaactgcccg tacggtgcct ttaattttgt tggttttaca ggcgtcaata attgcctgta
     7441 aattacttcc gttgccggaa ataagcacca caatattcat tattcgataa ccacgcgttg
     7501 ttcggaatca gaggctttga tgataccgat tttccacgcg ttttcaccgt tggcattgag
     7561 cagggcgagg gctttgtcca cttccggagc aggcagggca ataatcatcc cgacgccgca
     7621 gttgaaggtg cgatacattt catggcgttc aacgttacct gccgtttgca gccagttgaa
     7681 cacttccggc cactgccagg aagattcatc aatcactgcc tgggtattat ctggcaatac
     7741 gcgcggaatg ttttcccaga agccgccgcc ggtcaggtgc gcaatggcat gcacatcgac
     7801 cttttcaatc aactccagca ctgacttaac gtaaatgcgg gtcggtgcca gcagatgatc
     7861 ggctaatggc ttaccatcaa gttcggtggt ttgcggatca caaccgctga cttcaagaat
     7921 tttgcgcacc agcgaatagc cgttcgagtg cggaccgctg gaaccgagtg caatcagaac
     7981 atcgccgtcg ctgactttag agccgtcgat gatttctgat ttttctacca cgcccacgca
     8041 gaaacccgcg acatcgtaat cttcaccgtg atacatcccc ggcatttctg ccgtttcgcc
     8101 acccaccagt gaacagcccg attgcagaca accttccgcg atgccgctga tcaccgctga
     8161 agcggtatca acatccagtt ttccggttgc gtaatagtcg aggaaaaaca gcggttctgc
     8221 accttgcacc accaggtcat taacgcacat ggcgaccaga tcaataccaa tggtgtcgtg
     8281 acgttttaag tccattgcca gacgcagctt ggtacctacg ccgtcagtgc cggaaaccag
     8341 cacgggttca cgatattttt gcggcaatgc acacagcgca ccgaagccgc ccagaccgcc
     8401 catcacttcc ggacgacgcg ttttcttcac tacgcctttg attcttccaa ccagagcatt
     8461 acccgcgtca atatcaacac cggcatcttt gtagctaaga gaggttttat cggtcactgc
     8521 ttgggtcccc acgcgttact tgcggtagaa aaataaaatt cggcgcaatt ctaacaggga
     8581 aagcaaacgt ttgcgagact gctttacaca acctttttgc acgtcttttc cccagacgcg
     8641 cggcgaaaga agacttgtgc cagggcaaag gtt
//
LOCUS       DAOFQD010000170         8668 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.170, whole genome shotgun sequence.
ACCESSION   DAOFQD010000170 DAOFQD010000000
VERSION     DAOFQD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.170"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(309..572)
                     /gene="rpsT"
                     /locus_tag="QBF46_004296"
     CDS             complement(309..572)
                     /gene="rpsT"
                     /locus_tag="QBF46_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDQ6676407.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            675..893
                     /locus_tag="QBF46_004297"
     CDS             675..893
                     /locus_tag="QBF46_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDQ6676408.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            901..1842
                     /gene="ribF"
                     /locus_tag="QBF46_004298"
     CDS             901..1842
                     /gene="ribF"
                     /locus_tag="QBF46_004298"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDQ6676409.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1885..4701
                     /gene="ileS"
                     /locus_tag="QBF46_004299"
     CDS             1885..4701
                     /gene="ileS"
                     /locus_tag="QBF46_004299"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDQ6676410.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4701..5195
                     /gene="lspA"
                     /locus_tag="QBF46_004300"
     CDS             4701..5195
                     /gene="lspA"
                     /locus_tag="QBF46_004300"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDQ6676411.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5320..5769
                     /gene="fkpB"
                     /locus_tag="QBF46_004301"
     CDS             5320..5769
                     /gene="fkpB"
                     /locus_tag="QBF46_004301"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDQ6676412.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5771..6721
                     /gene="ispH"
                     /locus_tag="QBF46_004302"
                     /gene_synonym="lytB"
     CDS             5771..6721
                     /gene="ispH"
                     /locus_tag="QBF46_004302"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDQ6676413.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6787..7701
                     /gene="rihC"
                     /locus_tag="QBF46_004303"
     CDS             6787..7701
                     /gene="rihC"
                     /locus_tag="QBF46_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDQ6676414.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVKCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTMVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAP"
     gene            7868..>8668
                     /gene="dapB"
                     /locus_tag="QBF46_004304"
     CDS             7868..>8668
                     /gene="dapB"
                     /locus_tag="QBF46_004304"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDQ6676415.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKEDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     GHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDV"
BASE COUNT         1979 a         2182 c         2458 g         2049 t
ORIGIN      
        1 tgtataggaa tttaattcaa aaatagagtg gtcttaagtt taatacctca atgtgtatca
       61 caatatccac attctttgta ggggagcctg gagattgagt agatattctt gctcagaatg
      121 tatcagccga tggttctacg attcttaagc cacgaagagt tcagatagta taacggcatg
      181 tctgcaagaa cctgcatttt ttgactatct ggcaaccggc agtgtgttct ctcacgcatc
      241 acaaaagcag caggcataaa aaaacccgct tgcgcgggct ttttcacaaa gcttcagcaa
      301 attggcgatt aagccagttt gttgatctgt gcagtcaggt tagccttatg acgtgcagct
      361 ttgtttttgt ggatcagacc tttagcagcc tgacggtcca cgatcggttg catttcgtta
      421 aatgctttct gtgcagcagc tttgtcgcca gcttcgatag ctgcgtatac tttcttgatg
      481 aaagtacgca tcatagagcg acggcttgcg ttgtgcttac gagccttttc agactgaatg
      541 gcgcgcttct tagctgattt gatattagcc aaggtccaac tcccaaatgt gttctatatg
      601 gacaattcaa aggccgagga atatgccctt ttagccttct tttgtcaatg gatttgtgca
      661 aataagcgcc gttaatgtgc cggcactcgt tacgtagtga tggcgcagga ttctaccagc
      721 ttgcggggtg tgaatacagc ttttccgcga taaaaattgc agcaggcggt cactttcttc
      781 ccgtgatttg cgccatggca atgaaaagcc acttctttct gatttcggta ctcaatcgcc
      841 ggttaacctt gaccgctgta caaggtatac tcggacgatt ttcactgttt tgagccagac
      901 atgaagctga tacgcggcat acataatctc agccaggccc cgcaagaagg gtgtgtgctg
      961 actattggta atttcgacgg cgtgcatcgc ggtcatcgcg cgctgttaca gggcttgcag
     1021 gaagaagggc gcaagcgcaa cttaccggtg atggtgatgc tttttgaacc tcaaccactg
     1081 gaactgtttg ctaccgataa agccccggca agactgaccc ggctgcggga aaaactgcgt
     1141 taccttgcag agtgtggcgt tgattacgtg ctgtgcgtgc gtttcgacag gcgtttcgcg
     1201 gcgttaaccg cgcaaaattt catcagcgat cttctggtga agcatttgcg cgtaaaattt
     1261 cttgccgtag gtgatgattt ccgctttggc gctggtcgtg aaggcgattt cttgttatta
     1321 cagaaagctg gcatggaata cggcttcgat atcaccagta cgcaaacttt ttgcgaaggt
     1381 ggcgtgcgca tcagcagcac cgccgtgcgt caggcccttg cggatgacaa tctggctctg
     1441 gcagagagtt tactggggca cccgtttgcc atctccgggc gtgtagtcca cggtgatgaa
     1501 ttagggcgca ctataggttt cccgacggcg aatgtaccgc tgcgccgtca ggtttccccg
     1561 gtgaaagggg tttatgcggt agaagtgctg ggcctcggtg aaaagccgtt acccggcgtg
     1621 gcaaacatcg gaacacgccc aacggttgcc ggtattcgcc agcagctgga agtgcatttg
     1681 ttagatgttg caatggacct ttacggtcgc catatacaag tagtgctgcg taaaaaaata
     1741 cgcaatgagc agcgatttgc gtcgctggac gaactgaaag cgcagattgc gcgtgatgaa
     1801 ttaaccgccc gcgaattttt tgggctaaca aaaccggctt aagcctgtta tgtaatcaaa
     1861 ccgaaatacg gaaccgagaa tctgatgagt gactataaat caaccctgaa tttgccggaa
     1921 acagggttcc cgatgcgtgg cgatctcgcc aagcgcgaac ccggaatgct ggcgcgttgg
     1981 actgatgatg atctgtacgg catcatccgt gcggctaaaa aaggcaaaaa aaccttcatt
     2041 ctgcatgatg gccctcctta tgcgaatggc agcattcata ttggtcactc ggttaacaag
     2101 attctgaaag acattatcgt gaagtccaaa gggctttccg gttatgactc gccgtatgtg
     2161 cctggctggg actgccacgg tctgccgatc gagctgaaag tcgagcaaga atacggtaag
     2221 ccgggtgaga aattcaccgc cgccgagttc cgcgccaagt gccgcgaata cgcggcgacc
     2281 caggttgacg gtcaacgcaa agactttatc cgtctgggcg tgctgggcga ctggtcgcac
     2341 ccgtacctga ccatggactt caaaactgaa gccaacatca tccgcgcgct gggcaaaatc
     2401 atcggcaacg gtcacctgca caaaggcgcg aagccagttc actggtgcgt tgactgccgt
     2461 tctgcgctgg cggaagcgga agttgagtat tacgacaaaa cttctccgtc catcgacgtt
     2521 gctttccagg cagtcgatca ggatgcattg aaagcaaaat ttgccgtaag caacgttaac
     2581 ggcccaatct cgctggtaat ctggaccacc acgccgtgga ctctgcctgc caaccgcgca
     2641 atctctattg caccagattt cgactatgcg ctggtgcaga tcgacggtca ggccgtgatt
     2701 ctggcgaaag atctggttga aagcgtaatg cagcgtatcg gcgtgaccga ttacaccatt
     2761 ctcggcacgg taaaaggtgc ggagcttgag ctgctgcgct ttacccatcc gtttatgggc
     2821 ttcgacgttc cggcaatcct cggcgatcac gttaccctgg atgccggtac cggtgccgtt
     2881 cacaccgcgc ctggccacgg cccggacgac tatgtgatcg gtcagaaata cggcctggaa
     2941 accgctaacc cggttggccc ggacggcact tatctgccgg gcacttatcc gacgctggat
     3001 ggcgtgaacg tcttcaaagc gaacgacatc gtcgttgcgc tgctgcagga aaaaggcgcg
     3061 ctgctgcacg ttgagaaaat gcagcacagc tatccgtgct gctggcgtca caaaacgccg
     3121 atcatcttcc gcgcgacgcc gcagtggttc gtcagcatgg atcagaaagg tctgcgtgcg
     3181 cagtcactga aagagatcaa aggcgtgcag tggatcccgg actggggcca ggcgcgtatc
     3241 gagtcgatgg ttgctaaccg tcctgactgg tgtatctccc gtcagcgcac ctggggtgta
     3301 ccgatgtcac tgttcgtgca caaagacacg gaagagctgc atccgcgtac ccttgaactg
     3361 atggaagaag tggcaaaacg cgttgaagtc gatggcatcc aggcgtggtg ggatctcgat
     3421 gcgaaagaga tcctcggcga cgaagctgat cagtacgtga aagtgccgga cacattggat
     3481 gtatggtttg actccggatc tacccactct tctgttgttg acgtgcgtcc ggaatttgcc
     3541 ggtcacgcag cggacatgta tctggaaggt tctgaccaac accgcggctg gttcatgtct
     3601 tccctaatga tctccaccgc gatgaagggt aaagcgccgt atcgtcaggt actgacccac
     3661 ggctttaccg tggatggtca gggccgcaag atgtctaaat ccatcggcaa taccgtttcg
     3721 ccgcaggatg tgatgaacaa actgggcgcg gatattctgc gtctgtgggt ggcatcaacc
     3781 gactacaccg gtgaaatggc cgtttctgac gagatcctga aacgtgctgc cgatagctat
     3841 cgtcgtatcc gtaacaccgc gcgcttcctg ctggcaaacc tgaacggttt tgatccagca
     3901 aaagatatgg tgaaaccgga agagatggtg gtactggatc gctgggccgt aggttgtgcg
     3961 aaagcggcac aggaagacat cctcaaggcg tacgaagcat acgatttcca cgaagtggta
     4021 cagcgtctga tgcgcttctg ctccgttgag atgggttcct tctacctcga catcatcaaa
     4081 gaccgtcagt acaccgccaa agcggacagt gtggcgcgtc gtagctgcca gactgcgcta
     4141 tatcacatcg cagaagcgct ggtgcgctgg atggcaccaa tcctctcctt caccgctgat
     4201 gaagtgtggg gctacctgcc gggcgaacgt gaaaaatacg tcttcaccgg tgagtggtac
     4261 gaaggcctgt ttggcctggc agacagtgaa gcgatgaacg atgcgttctg ggacgagctg
     4321 ttgaaagtgc gtggcgaagt gaacaaagtc attgagcaag cgcgtgccga caagaaagtg
     4381 ggtggctcgc tggaagcggc agtaaccttg tatgcagaac cggaactgtc ggcgaaactg
     4441 accgcgctgg gcgatgaatt acgatttgtc ctgttgacct ccggcgctac cgttgcagac
     4501 tataacgacg cacctgctga tgctcagcag agcgaagtac tcaaagggct gaaagtcgcg
     4561 ttgagtaaag ccgaaggtga gaagtgccca cgctgctggc actacaccca ggatgtcggc
     4621 aaggtggcgg aacacgcaga aatctgcggc cgctgtgtca gcaacgtcgc cggtgacggt
     4681 gaaaaacgta agtttgcctg atgagtcaat cgatctgttc aacagggcta cgctggctgt
     4741 ggctggtggt agtcgtgctg attatcgatc tgggcagcaa atacctgatc ctccagaact
     4801 ttgctctggg ggatacggtc ccgctgttcc cgtcgcttaa tctgcattat gcgcgtaact
     4861 atggcgcggc gtttagtttc cttgccgata gcggcggctg gcagcgttgg ttctttgccg
     4921 gtattgcgat tggtattagc gtgatcctgg cagtgatgat gtatcgctcg aaggccacgc
     4981 agaagctaaa caatatcgct tacgcgctga ttattggcgg cgcgctgggc aacctgttcg
     5041 accgcctgtg gcacggcttc gttgtcgata tgatcgactt ctacgtcggc gactggcact
     5101 tcgccacctt caaccttgcc gatactgcca tctgtgtcgg tgcggcactg attgtgctgg
     5161 aaggtttttt gccttctaga gcgaaaaaac aataataaac cctgccggat gcgatgctga
     5221 cgcatcttat ccggcctaca gattgctgcg aaatcgtagg ccggataagg cgtttacgcc
     5281 gcatccggca aaaatcctta aatataagag caaacctgca tgtctgaatc tgtacagagc
     5341 aatagcgccg tcctggtgca cttcacgcta aaactcgacg atggcaccac cgccgagtct
     5401 acccgcaaca acggtaaacc ggcgctgttc cgcctgggtg atgcttctct ttctgaaggg
     5461 ctggagcaac acctgttggg gctgaaagtg ggcgataaaa ccaccttctc gttggagcca
     5521 gatgcggcgt ttggcgtgcc gtcaccggac ctgattcagt acttctcccg ccgtgaattt
     5581 atggatgcag gcgagccaga aattggcgca atcatgcttt ttaccgcaat ggatggcagt
     5641 gagatgcctg gcgtgatccg cgaaattaac ggcgactcca ttaccgttga tttcaaccat
     5701 ccgctggccg ggcagaccgt tcattttgat attgaagtgc tggaaatcga tccggcactg
     5761 gaggcgtaac atgcagatcc tgttggccaa cccgcgtggt ttttgtgccg gggtagaccg
     5821 cgctatcagc attgttgaaa acgcgctggc catttacggc gcaccgatat atgtccgtca
     5881 cgaagtggtg cataaccgct acgtggtcga tagcctgcgc gagcgtgggg ctatctttat
     5941 tgagcagatt agcgaagtgc cggacggcgc aatcctgatt ttctccgcac acggtgtttc
     6001 tcaggcggta cgtaacgaag cgaaaagccg tgatttgacg gtattcgacg ccacctgccc
     6061 gctggtgacc aaagtgcata tggaagtcgc ccgcgctagt cgccgtggcg aagaatctat
     6121 tctcatcggt cacgccgggc acccggaagt ggaaggcacg atgggtcagt acagcaaccc
     6181 ggaaggggga atgtatctgg ttgaatcacc agacgatgtg tggaaactga cggtcaaaaa
     6241 cgaagagaaa ctctccttta tgacccagac cacgctgtcg gtggatgaca cgtctgatgt
     6301 gatcgacgcg ctgcgtaaac gcttcccgaa aattgtcggt ccgcgcaaag atgacatctg
     6361 ctacgccacg actaaccgac aggaagcggt acgtgccctg gccgaacagg cggaagttgt
     6421 gctggtggtc ggttcgaaaa actcctctaa ctccaaccgt ctggcggagc tggcccagcg
     6481 tatgggcaaa agcgcgtttt tgattgatga tgcgaaagat atccaggaag agtgggtgaa
     6541 agaggttaaa tgcgtcggcg tgactgcggg cgcatcggct ccggatattc tggtgcagaa
     6601 tgtggtggca cgtttgcaac agctgggcgg tggtgaagcc attccgctgg aaggacgtga
     6661 agaaaacatt gttttcgaag tgccgaaaga gctgcgtgtc gatattcgtg aagtcgatta
     6721 agtcattagc agcctaagtt atgcgaaaat gccggtcttg ttaccggcat tttttatgga
     6781 gaaaacatgc gtttacctat cttcctcgat actgaccccg gcattgacga tgccgtcgcc
     6841 attgccgctg cgatttttgc ccccgaactc gacctgcaac tgatgaccac cgtcgcgggt
     6901 aatgtctcgg ttgagaaaac cacccgcaac gccctgcaac tgctgcattt ctggaatgcg
     6961 gagattccgc tcgcccaggg agccgctgtg ccgctggtac gcgcaccgcg tgatgcagca
     7021 tctgtgcacg gcgaatcggg aatggctggc tacgactttg ttgagcacaa ccgaaagcca
     7081 atcgggatac cagcgtttct ggcgattcgt gatgccctga tgcgtgcacc agagcctgtt
     7141 accctggtgg ccattggccc gttaaccaat attgcgctgt tactttcaca atgcccggaa
     7201 tgtaaaccgt atattcgtcg tctggtgatc atgggcggtt cttctggtcg cggcaactgt
     7261 acaccaaatg ccgagtttaa tatcgctgcc gatccagaag cggctgcctg tgtcttccgt
     7321 agcggaatcg aaatcgtcaa gtgcggtctg gatgtcacca atcaggcaat attaacccct
     7381 gactatctcg ctactttgcc ggagttgaat cgcaccggaa aaatgctcca cgccctgttt
     7441 agccactacc gtagcggcag tatgcaaagc ggtctgcgaa tgcacgatct ctgcgccatc
     7501 gcctggctgg tgcgcccgga cttattcacc ctcaagccgt gttttgtcgc ggtagaaact
     7561 caaggcgaat ttacctcagg cacgacgatg gttgatatcg acggttgcct gggcaaacca
     7621 gccaatgtac aggtagcatt ggatctggat gtaaaaggct tccagcagtg ggtggctgag
     7681 gtgctggctc tggcgccgta acctgtcaca tgttattggc atgcagtcat tcatcgactc
     7741 atgcctttca ctgatatccc tccctgttta tcattaattt ctaattatca gcgtttttgg
     7801 ctggcggcgt agcgatgcgc tggttactct gaaaacggtc tatgcaaatt aacaaaagag
     7861 aatagctatg catgatgcaa acatccgcgt tgccatcgcg ggagccgggg ggcgtatggg
     7921 ccgccaattg attcaggcgg cgctggcatt agaaggcgtg cagctgggcg cggcgctgga
     7981 gcgcgaagga tcttctttac tgggcagcga cgccggtgag ctggctggtg cagggaaaac
     8041 aggcgttacc gtgcaaagca gcctcgatgc ggtaaaagaa gattttgatg tgtttatcga
     8101 ttttacccgt ccggaaggta cgctgaacca tctcgctttt tgccgccagc atggcaaagg
     8161 gatggtgatc ggcactacgg ggtttgacga agccggtaaa caggcgattc gtgacgccgc
     8221 cgccgatatt gcgattgtct ttgccgccaa ttttagcgtt ggcgttaacg tcatgcttaa
     8281 gctgctggag aaagcagcca aagtgatggg tgactacacc gatatcgaaa ttattgaagc
     8341 acatcatgga cataaagttg atgcgccgtc aggcaccgca ctggcaatgg gagaggcgat
     8401 cgcccacgcc cttgataaag atctgaaaga ttgcgcggtc tacagtcgtg aaggccacac
     8461 cggtgaacgt gtgcctggca ccattggttt tgccaccgtg cgtgcaggtg acatcgttgg
     8521 cgaacatacc gcgatgtttg ccgatattgg cgagcgtctg gagatcaccc ataaggcgtc
     8581 tagccgcatg acatttgcta acggcgcggt aagatcggct ttgtggttga gtggtaagga
     8641 aagtggtctt tttgatatgc gagatgtg
//
LOCUS       DAOFQD010000171         8543 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.171, whole genome shotgun sequence.
ACCESSION   DAOFQD010000171 DAOFQD010000000
VERSION     DAOFQD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.171"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            1..859
                     /locus_tag="QBF46_004305"
                     /pseudo
     CDS             1..859
                     /locus_tag="QBF46_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            1222..2259
                     /gene="dusA"
                     /locus_tag="QBF46_004306"
     CDS             1222..2259
                     /gene="dusA"
                     /locus_tag="QBF46_004306"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDQ6676416.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2393..2635
                     /gene="pspG"
                     /locus_tag="QBF46_004307"
     CDS             2393..2635
                     /gene="pspG"
                     /locus_tag="QBF46_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDQ6676417.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2801..3784)
                     /locus_tag="QBF46_004308"
     CDS             complement(2801..3784)
                     /locus_tag="QBF46_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDQ6676418.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3867..5282
                     /gene="dnaB"
                     /locus_tag="QBF46_004309"
     CDS             3867..5282
                     /gene="dnaB"
                     /locus_tag="QBF46_004309"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDQ6676419.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5335..6414
                     /gene="alr"
                     /locus_tag="QBF46_004310"
     CDS             5335..6414
                     /gene="alr"
                     /locus_tag="QBF46_004310"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDQ6676420.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6667..7860
                     /gene="tyrB"
                     /locus_tag="QBF46_004311"
     CDS             6667..7860
                     /gene="tyrB"
                     /locus_tag="QBF46_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDQ6676421.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(8363..8543)
                     /locus_tag="QBF46_004312"
                     /pseudo
     CDS             complement(8363..8543)
                     /locus_tag="QBF46_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2140 a         1971 c         2151 g         2281 t
ORIGIN      
        1 atatatatgg taaatctatt gattatgcag cgctgcgtca tcgggatata attattgcaa
       61 aaatagattt gtttattcaa cgaatcacgc ataatttatg gcatgcacgt aaaaaaatgt
      121 gtttttaatt gagcaaataa atgatctcaa aatgtgggtc aataagtata ttgacgattg
      181 cactgatgag gatttaaacg atcgtgactt tattgcatca gttgttgacc gggctatttt
      241 tcatttcgcg attaatagta tatgtaatcc tggggataat aaagatgcaa tgcccattga
      301 acaatgtact tttgatgtag aaactaagaa tgaccttccc tccacggttc agctatttta
      361 tgaggaatct aaggataatg aacctttagc gaatatacat tttcaagcaa taggttctgg
      421 ttttttaacg tttgttaatg cctgccagga acatgatgac aacagcttaa aattatttgc
      481 ttcgctgtta atttcacttt catattctag tgcctacgca gatttatcag aaacagtgta
      541 tattaatgaa aataatgaga gctacctgaa agctcagttt gaaaaattat ctcaacgtga
      601 tatgaagaag tacctgggag agatgaagcg tctggcagat gggggagaaa tgaattttga
      661 tggctatctg gataagatgt cacatctggt gaatgaagga acgctcgatc ctgatatttt
      721 aagcaaaatg cgagatgctg caccacaatt aattagcttc gcgaagtcgt ttgacccaac
      781 ctcaaaggaa gagattaaaa tacttacaga cacttctaaa ttaatttatg atttgttcgg
      841 ggttaaatcg gagaaataat atgtgaagtt cttcgatagt atggaaggca ttatataaaa
      901 ggaaccaata tttattgggt tcttttttct ctatcaatgc tattagcagg gagatatatc
      961 accagagttt aatgtgtgat tttttattta tcgtcgaacc tggattgttt atcattggcc
     1021 ttaacaaagt taacggctaa taagattatt tccatcactt cgtgagagct tcatgccttg
     1081 agaggatctc aattttcttt tgcaatgaga caggcgcttc ctgttgttat ggtatagtac
     1141 cccgctattg agcctcctga acagtgatgc tgaataacat aaccccatga tatatcgata
     1201 aaataatctc tacatttgaa aatgcacggt aattctgaaa tgcaaaaaat caaccaaacc
     1261 agcgcaatgc ctgaaaaaac tgacgttcac tggagtggtc ggtttagcgt tgcaccaatg
     1321 ctcgactgga cggacagaca ttgccgctat ttcttgcgtc tgctttcccg caatacgttg
     1381 ctgtatactg aaatggtaac tacaggggcg attattcacg gtaaaggtga ttacctggcg
     1441 tatagtgaag aagaacatcc ggtagcgttg caactcggcg gtagcgatcc ggcggcgctg
     1501 gcgcagtgtg cgaagctggc agaagcgcgt ggatatgatg agatcaacct gaatgtcggc
     1561 tgcccgtctg atcgggtgca gaacggcatg tttggtgcgt gtctgatggg taatgcgcag
     1621 ctggttgccg actgcgtgaa agcgatgcgc gatgtggtgt cgattccagt gacggtgaaa
     1681 acgcgtattg gcatcgatga ccaggacagc tatgaatttc tctgcgattt catcaacact
     1741 gtttccggca aaggcgagtg tgagatgttt atcatccacg cacgtaaagc ctggctttcg
     1801 gggttaagcc cgaaagaaaa ccgtgaaatc ccgccgctcg attatccgcg tgtgtatcaa
     1861 ctgaagcgtg actttccgca tctgacaatg tcgattaacg gtggtatcaa gtcgctggaa
     1921 gaggccaaag cacacctgca acatatggat ggcgtgatgg tcgggcgcga ggcgtatcag
     1981 aatccgggta ttctggcggc ggtagaccgg gagatctttg gttcctcgga taccgatgcc
     2041 gatccggtgg cggtagtgcg cgccatgtat ccgtacattg agcgtgaact cagccagggg
     2101 acgtatctcg gccatattac ccggcatatg ttgggcttgt tccagggtat tcctggcgcg
     2161 cggcagtggc ggcgttattt aagtgaaaat gcccataaag cgggtgcaga cattaatgtg
     2221 ctggaacacg cgctcaaact ggtggcggat aagcgttaac ttttcaccaa aaagtagtca
     2281 aattcaccac gccctgcgca ccgtcgcggg gcgttttgct gttaaatcaa tagattattt
     2341 ttggcatgat tcttgtaatg ccagcaagag atttcatatt tgggagagca tcatgctgga
     2401 actacttttt gtgattggct tttttgtcat gctgatggtc accggcgttt cgttgctggg
     2461 cattatcgcc gcgctggttg tggcgacggc cattatgttc ctcggcggta tgctggcatt
     2521 gatgattaag ttgctgccgt ggttactact ggcgattgcg gtggtgtggg ttattaaggc
     2581 gattaaagca ccaaaagtgc cgaaatatca gcgttatgac cgctggcgtt actaagggat
     2641 tgtgcggatg atcacaacct aaggttttat ccttagaaca aaataggaat tgataatcaa
     2701 atctgtcact attgcgcctc taacagattc atcgtgctgt accctacata cagccgaact
     2761 ataaaaagaa agggcttccc aggtggaagc cctatttctt ttatggaatc agcaggctgg
     2821 aaccttgcgt cgcccggctt tccagaatct catgcgcacg ctgcgcatcc ttcagcggat
     2881 atttctgctg ctcggcgaca tcgaccttaa tcacaccgct ggcaatcaaa gagaacagtt
     2941 cattactggc ctcggttaat tcctcccgcg tggtgatata gccttgcagg gaagggcgtg
     3001 tcacatacaa cgagcctttt tgattgagaa tgcctaagtt cacaccggta accgcacctg
     3061 atgagttgcc aaaactgacc attaagccgc ggcgttgcag gcaatccagc gaccgttccc
     3121 aggtgtctct gcccacggaa tcgtacacta cgcgcacttt cttaccgccg gtgatctctt
     3181 tcaatcgctc gaccagattc tcttcacgat agttaataac ctgccacgcg ccggctttta
     3241 gcgcgctctg cgctttttgc gcggttccta cggtgccgat aagtttcgcg cccagggctt
     3301 ttgcccactg gcaggcaatt aagccaacgc cgccagccgc tgcgtggaac aggaactgtt
     3361 catcgggttt aatttcatag gttttgcgca gcagataata aaccgttaag cctttcagga
     3421 aagatgctgc agcttgctca aaagaaattg ccgcaggcag aatcgccgct ttatccgcat
     3481 taatgttatg cacagagctg taagcgccta acgccgactg cgcatagact acacgatcgc
     3541 ctgccttaat atgctttaca ccactgccga ctttactcac gatgcctgcc gcttcggtac
     3601 ctaatccgct gggtagtgat ggcggcgggt aaaggccgct gcggatatac gtgtcgataa
     3661 aattgatgcc gatggcttta ttttcgacct ggatttcatt ctctgccgga tcggcaggag
     3721 tgaactctac ggcttgaagt acttccgggc caccgtgctt gtgaaattca attcgtgttg
     3781 ccatgtgtcc tccagaacgt atcgtcaggg tctgcttcat atggtaaagt ttcgacccat
     3841 tctttatctc ggtaactcca ttcactatgg caggaaataa acccttcaac aaacagcagg
     3901 ctgaaccccg cgaacgcgat ccacaagttg ccgggctgaa agtgcctccg cactcgatcg
     3961 aagcggagca gtcggtgttg ggcggtttaa tgctggataa cgaacgctgg gacgatgtag
     4021 ccgagcgtgt ggtggcagac gatttttaca cccgcccaca ccgccatatc tttactgaaa
     4081 tggcgcgttt gcaggaaagc ggtagtccta tcgatctgat tacccttgcg gaatcgctgg
     4141 aacgccaggg gcaactcgat agcgtcggtg gttttgctta tctggcagag ctgtcaaaaa
     4201 atacgccaag tgcggcgaac atcagtgctt atgctgacat cgtgcgtgaa cgtgccgttg
     4261 tccgtgagat gatctcggtt gcgaatgaga ttgctgaagc cggttttgat ccgcaggggc
     4321 gtaccagcga agatctgctg gaccttgctg aatcccgcgt ctttaaaatt gccgaaagtc
     4381 gtgcaaacaa agacgaaggg ccgaagaaca tcgccgatgt gctcgacgca accgtggcgc
     4441 gtattgagca gttgtttcag cagccacacg atggcgttac cggagtaaac accggttatg
     4501 acgatctcaa caaaaaaacc gctggcttgc agccgtcgga tttgatcatc gtcgccgcgc
     4561 gtccgtcgat gggtaaaaca acatttgcga tgaacctcgt cgaaaacgcg gcgatgttgc
     4621 aggataaacc agtacttatc ttctcgctgg agatgccatc agaacagatc atgatgcgtt
     4681 ctctggcgtc gctgtcgcgc gttgaccaga ctaaaatccg taccgggcag ctcgatgatg
     4741 aagactgggc gcgcatttcc ggcaccatgg gtattttgct cgaaaaacgc aatatctata
     4801 tcgatgactc ttccggcctg acgccaacgg aagtgcgttc ccgcgcacgc cgtattgccc
     4861 gtgaacacgg cggcatcggg cttatcatga tcgactacct gcaactgatg cgcgtaccgg
     4921 cgctttccga taaccgtacg ctggaaattg cagaaatctc ccgctcgctg aaagcactgg
     4981 cgaaagaact gaacgtgccg gtggtggcgc tgtcccagtt gaaccgttct ctggaacaac
     5041 gtgccgacaa acgcccggtc aactccgacc tgcgtgaatc tggctctatc gagcaggatg
     5101 cggacttgat catgtttatc tatcgtgatg aggtgtatca cgaaaacagt gatttaaaag
     5161 gcatcgcgga aattattatc ggtaaacaac gtaacggccc aatcgggacg gtacgcctga
     5221 cctttaacgg tcaatggtcg cgcttcgaca actatgcggg gccgcagtac gacgacgaat
     5281 aataattatt ttatgaatta ggtaattaaa gcaaacactt atcaaggaac acaaatgcaa
     5341 gcggcaactg ttgtgattaa ccgccgcgct ctgcgacaca acctgcaacg tcttcgtgaa
     5401 ctggcacctg ccagtaaaat ggttgcggtg gtgaaagcga acgcttatgg tcacggtctt
     5461 cttgagaccg cgcgaacgct ccccgatgct gacgcctttg gcgtagcccg tctcgaagaa
     5521 gctctgcgac tgcgtgcggg gggaatcacc aaacctgtac tgttactcga aggctttttt
     5581 gatgccagag atctgccgac gatttctgcg caacattttc ataccgccgt gcataacgaa
     5641 gaacagctgg ctgcgctgga agaggctagc ctggacgagc cggttaccgt ctggatgaaa
     5701 ctcgataccg gtatgcaccg tctgggcgta aggccggaac aggctgaggc gttttatcat
     5761 cgcctgaccc agtgcaaaaa cgttcgtcag ccggtgaata tcgtcagcca ttttgcgcgc
     5821 gcggatgaac caaaatgtgg cgcaaccgag aaacaactcg ctatctttaa taccttttgc
     5881 gaaggcaaac ctggtcaacg ttccattgcc gcatcgggtg gcattctgct gtggccacag
     5941 tcgcattttg actgggtgcg cccgggcatc attctttatg gcgtctcgcc gctggaagat
     6001 cgctccaccg gtgccgattt tggctgtcag ccagtgatgt cactaacctc cagcctgatt
     6061 gccgtgcgtg agcataaagc cggagaacct gttggttatg gtggaacctg ggtaagcgaa
     6121 cgtgataccc gtcttggcgt agtcgcgatg ggctatggcg atggttatcc gcgcgccgcg
     6181 ccgtccggta cgccagtgct ggtgaacggt cgcgaagtac cgattgtcgg gcgcgtggcg
     6241 atggatatga tctgcgtaga cttaggtcca caggcgcagg acaaagccgg ggatccggtc
     6301 attttatggg gcgaaggttt gcccgtagaa cgtatcgctg aaatgacgaa agtaagcgct
     6361 tacgaactta ttacgcgcct gacttcaagg gtcgcgatga aatacgtgga ttaatcgttc
     6421 tgtaatattt gattgtctgt gccggatgcg gcgtgaatgc cttatccggc caataaaatc
     6481 ctaaaaattc aataagttga tgttctttca tgctcttata aaggtcgtgc ctctggcgga
     6541 tgtacgtttg tcatgagtct cactctgttg ctaattgccg ttcgctcctg aacatccact
     6601 cgatcttcgc cttcttccgg tttattgtgt tttaaccacc tgcccgtaaa cctggagaac
     6661 catcgcgtgt ttcaaaaagt tgacgcctac gctggcgacc cgattcttac gcttatggag
     6721 cgttttaaag aagaccctcg cagcgacaaa gtgaatttaa gtatcggtct gtactacaac
     6781 gaagacggaa ttattccaca actgcaagcc gtggcggagg cggaagcgcg cctgaatgcg
     6841 cagcctcatg gcgcttcgct ttatttaccg atggaagggc ttaacagcta tcgccatgcc
     6901 attgcgccgc tgctgtttgg tgcggaccat ccggtactga aacaacagcg cgtagcaacc
     6961 attcaaaccc ttggcggctc cggggcattg aaagtgggcg cggatttcct gaaacgctac
     7021 ttcccggaat caggcgtctg ggtcagcgat cctacctggg aaaaccacgt agcaatattc
     7081 gccggggctg gattcgaagt gagtacttac ccctggtatg acgaagcgac taacggcgtg
     7141 cgctttaatg acctgttggc gacgctgaaa acattacctg cccgcagtat tgtgttgctg
     7201 catccatgtt gccacaaccc aacgggtgcc gatctcacta atgatcagtg ggatgcggtg
     7261 attgaaattc tcaaagcccg cgagcttatt ccattcctcg atattgccta tcaaggattt
     7321 ggtgccggta tggaagagga tgcctacgct attcgcgcca ttgccagcgc tggattaccc
     7381 gctctggtga gcaattcgtt ctcgaaaatt ttctcccttt acggcgagcg cgtcggcgga
     7441 ctttctgtta tgtgtgaaga tgccgaagcc gctggccgcg tactggggca attgaaagca
     7501 acagttcgcc gcaactactc cagcccgccg aattttggtg cgcaggtggt ggctgcagtg
     7561 ctgaatgacg aggcattgaa agccagctgg ctggcggaag tagaagagat gcgtactcgc
     7621 attctggcaa tgcgtcagga actggtgaag gtattaagca cagagatgcc agaacgcaat
     7681 ttcgattatc tgcttaatca gcgcggcatg ttcagttata ccggtttaag tgccgctcag
     7741 gttgaccgac tacgtgaaga atttggtgtc tatctcatcg ccagcggtcg catgtgtgtc
     7801 gccgggttaa atacggcaaa tgtacaacgt gtggcaaagg cgtttgctgc ggtgatgtaa
     7861 tgcaggaaag caggctggag ttacccagcc tgcagtgaaa ttaaactgtc gtcgctttca
     7921 ctatttcttt gtagatgatt tttttgatgc cgttgttcta cgtgagagat aataaaacgt
     7981 tgtcagttct tttattgtta agtttatccc aattatctgg aattcctttc cctatttttt
     8041 cgtgtggagt aataatttct gcacatctaa ccattattaa atttaataat aagtatcggg
     8101 tttgttacga tagaatgttt ctatatcctc aagaatatgc tttgccttat tacctgtgac
     8161 tttctgagat tcaattatag tatcgttaaa ttctaatgtt aaagagagct cttttttccg
     8221 ctttgtgtaa gtttaacctt atattgatta ccaatatcaa ataaaaaggt cctgtaatga
     8281 gaataatcac ctttaacctc ctcggcaaaa cagcaaagcg tgcaaatata cataaagtct
     8341 ttcttacccc tgattttgtt aactaaatca ataaatgcgt gaaaaacttt acttgcaata
     8401 gaacttgata cttcttctcc aggtctacaa aaatcttttg caatttttaa ctgtgtttct
     8461 atgtataaaa agaaatcttt ggcataagag aaatctttgg cataagagat atctttatta
     8521 tttagtgcat ctagctcaac agg
//
LOCUS       DAOFQD010000172         8441 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.172, whole genome shotgun sequence.
ACCESSION   DAOFQD010000172 DAOFQD010000000
VERSION     DAOFQD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.172"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(220..296)
                     /locus_tag="QBF46_004313"
     tRNA            complement(220..296)
                     /locus_tag="QBF46_004313"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Asp,seq:gtc)
     gene            complement(429..1160)
                     /gene="dnaQ"
                     /locus_tag="QBF46_004314"
     CDS             complement(429..1160)
                     /gene="dnaQ"
                     /locus_tag="QBF46_004314"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDQ6676422.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DDELAAHEARLDLVQKKGGSCLWRA"
     gene            1225..1692
                     /gene="rnhA"
                     /locus_tag="QBF46_004315"
     CDS             1225..1692
                     /gene="rnhA"
                     /locus_tag="QBF46_004315"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDQ6676423.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1689..2411)
                     /locus_tag="QBF46_004316"
     CDS             complement(1689..2411)
                     /locus_tag="QBF46_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDQ6676424.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2445..3200
                     /gene="gloB"
                     /locus_tag="QBF46_004317"
     CDS             2445..3200
                     /gene="gloB"
                     /locus_tag="QBF46_004317"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDQ6676425.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3272..4630
                     /gene="mltD"
                     /locus_tag="QBF46_004318"
     CDS             3272..4630
                     /gene="mltD"
                     /locus_tag="QBF46_004318"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDQ6676426.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YCIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4679..5449)
                     /locus_tag="QBF46_004319"
     CDS             complement(4679..5449)
                     /locus_tag="QBF46_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDQ6676427.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHAFGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGFFTSDIIMVDAHKAA"
     gene            complement(5527..6327)
                     /locus_tag="QBF46_004320"
     CDS             complement(5527..6327)
                     /locus_tag="QBF46_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDQ6676428.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6568..7482
                     /locus_tag="QBF46_004321"
     CDS             6568..7482
                     /locus_tag="QBF46_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6676429.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7479..8282)
                     /gene="dkgB"
                     /locus_tag="QBF46_004322"
     CDS             complement(7479..8282)
                     /gene="dkgB"
                     /locus_tag="QBF46_004322"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDQ6676430.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2268 a         2015 c         2038 g         2120 t
ORIGIN      
        1 tacattcatt taatcaatat aaatttattc tctggaaata tatttaagct gaatggttcg
       61 atatcattat tgaattttaa tgaatgagac aacaagcgag aatcacgcac ttacgccaga
      121 gtaaaatcat tgaacagaga aatggtggaa ttcaaataca aaaaaaccgc aaaattaaaa
      181 atcttgcggc tctctgaact cattttcatg agtgaatagt ggcggaacgg acgggactcg
      241 aacccgcgac cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccgcgt
      301 tgtgttccgt tgggaacgag gcgaatatta cggattgcct cgaccttcgt caacggtttt
      361 tctcatcttt tgaatcgttt gctgcaaaaa tcgcccaagt cgctattttt agcgcctttt
      421 acaggtattt atgcccgcca gaggcagctt ccgcccttct tctgcaccag atcaagacgg
      481 gcttcatgtg ctgcaagctc gtcatctgtc gcaaaaacaa cgcgtaactt acttgcctga
      541 cgtacaatgc gctgaattgt tgcttcacct tgttgctgtt gtgtctctcc ttccatcgca
      601 aaagccatcg acgtttgacc accggtcatc gccagataaa cttccgcaag gatctgggca
      661 tcgagtaatg ccccgtgtag cgttcgttta ctgttatcta tttcgtagcg ggcacataac
      721 gcatcgaggc tgttgcgctt accaggaaac attttcctcg ccaccgcaag gctatcggtg
      781 accttacaga aggtattggt cttcggaata tcgcgcttaa gcaacgaaaa ctcgtagtcc
      841 ataaagccga tatcgaacgc tgcgttatgg atcaccaact ccgcgccgcg gatatagtcc
      901 atgaactcat cggctacttc ggcaaacgtg ggcttatcga gcaaaaattc atcggcaata
      961 ccatgtacgc caaaggcttc cggatccacc agccgatcgg gtttgagata aacatggaag
     1021 ttattgcccg tcaggcgacg gttcaccact tcaacggcac caatctcaat gatcttgtgg
     1081 ccttcatagt gcgcaccaat ctggttcata ccggtggttt cggtatcgag aacgatctgg
     1141 cgtgtaattg cagtgctcat agcggtcatt tatgtcagac ttatcgtttt acagttcgat
     1201 tcaattacag gaagtctacc agagatgctt aaacaggtag aaattttcac cgatggttcg
     1261 tgtctgggca atccaggacc tgggggttac ggcgctattt tacgctatcg cggacgcgag
     1321 aaaaccttta gcgcaggcta cacccgcacc accaacaacc gtatggagtt gatggccgct
     1381 attgtcgcgc tggaggcgtt aaaagaacat tgcgaagtca ttttgagtac cgacagccag
     1441 tatgtccgcc agggtatcac ccagtggatc cataactgga aaaaacgtgg ctggaaaacc
     1501 gcagacaaaa aaccagtaaa aaatgtcgat ctctggcaac gtcttgatgc tgccctaggg
     1561 caacatcaaa tcaaatggga atgggttaaa ggccatgccg gacacccgga aaacgaacgc
     1621 tgtgatgaac tggctcgtgc cgcggcgatg aatcccacac tggaagatac aggctaccaa
     1681 gttgaagttt aagcctgtgg tttacgacat tgccgggtgg ctccaaccgc ctggcgaatt
     1741 cgtggcttgt ttttactctg tttcatcgga tttagcgtta aaggaatagt ccgtttccgg
     1801 gcaacaataa gttgtaagca accaagcgca ggaatatgcg cattcaatag ttttcctccg
     1861 tgtttgttcc acgggagaac gtggaaacgg ctggcgtgta gcacttcaaa attcaacaaa
     1921 gagagccagt ccagctgccg catcagagta aacatccggc tgttataggg cgaggttttg
     1981 cgcaataccg gcacaagttt gcgtaatccc attaaactga tgggattgaa gccactaatg
     2041 accagccagc catcatcaat caatacccga tcggcttcac gcaataaacg atgcggatcg
     2101 gtgcaccacg gcaatgtatg tgccagtaga caaacatcaa cggatttatc ggcaaaagga
     2161 agatgaagtg ggtccgcctg tacctggacg ggcattcctt gcgcagaaac attcacttga
     2221 tgagaaaccg cgcacgcttc gcaattgatt tctgcgctta aattgccaat cttaagcaga
     2281 tgaaaaccat acattttagt gaaccacggg ttgagctggc gctccagcgc cttgcgataa
     2341 agctctcccc agggcaaatc gccccagcaa tcaggagcca cgacagtttg agggactctt
     2401 gccggtttca tcacaacctt ccgtttcaca ctgagaggta atctatgaat cttaacagta
     2461 ttcccgcctt tgatgacaat tacatctggg ttttgaatga tgaagcaggt cgctgcctga
     2521 ttgtcgatcc cggagacgca gagccagtat taaacgccat tgccgccaat aactggcaac
     2581 cagaggccat atttctcacc caccatcacc acgatcacgt tggcggcgta aaagaactgg
     2641 tggaaaagtt tccgcaaatt gtggtgtatg gtccacaaga gacacaagat aagggaacaa
     2701 cacaggtagt caaagatggc gaaactgcct tcgttttagg gcatgaattt agtgtaattg
     2761 ccacaccggg tcacacttta ggacatatct gttacttcag caaaccttat ctattttgcg
     2821 gcgacacgct gttttctggt gggtgtggtc ggttgtttga agggacacca tcacaaatgt
     2881 atcaatcact taaaaagtta agtgcgttac ctgacgatac attggtatgt tgtgctcatg
     2941 aatatacgtt atcaaatatg aagtttgctt tgagtattct tccgcacgat ttgtccataa
     3001 atgattatta tcgtaaagtt aaggagttac gggcaaaaaa tcaaataaca ctacccgtaa
     3061 ttctgaaaaa tgagcggcaa attaatgttt ttttaagaac agaagatatt gatttaatta
     3121 atgtaattaa tgaagaaaca ttattgcaac aacctgaaga acgttttgca tggttaaggt
     3181 caaagaaaga taggttctga taaaactttc ttgtcatcgg ctccgttcgc cgttatgatc
     3241 ggtcgtcttt taagcaacta ttgacacaca catgaaggca aaagcgatat tactcgcctc
     3301 tgtcctgctc gtgggttgcc aaagtaccgg caacgttcaa cagcacgcac agagcctttc
     3361 tgcagctggt caaggggaag cagcaaagtt tacaagtcag gcacgatgga tggacgatgg
     3421 gacgtctatc gcgccagatg gtgacttgtg ggctttcatt ggcgacgagc taaagatggg
     3481 aattccggaa aatgaccgga ttcgcgaaca gaaacagaaa tatttacgca ataagagcta
     3541 tctccacgat gtaactttac gggcagagcc gtatatgtac tgtatagccg ggcaagttaa
     3601 aaaacgtaac atgcctatgg aactggtact actacccata gtggagagcg cttttgatcc
     3661 tcacgcaacg tctggcgcca atgccgcagg catctggcag atcattccga gcacggggcg
     3721 caattatggt ttgaaacaga cccgcaatta tgacgcgcgt cgcgatgttg ttgcttcaac
     3781 aactgccgcg ctgaacatga tgcagcgtct gaacaagatg tttgacggcg actggcttct
     3841 gaccgtagcg gcttataaca gcggcgaagg tcgggtcatg aaggcaatta aaacgaacaa
     3901 agcgcgtggg aaatccacgg acttctggtc gttaccgttg ccgcaggaaa cgaagcagta
     3961 cgtgcctaaa atgctggcat tgagtgatat tctcaaaaac agcaagcgtt atggcgtacg
     4021 tctgccaacg accgatgaaa gccgtgctct ggcgcgtgtg cacctgagca gcccggttga
     4081 aatggcgaag gttgcagata tggcggggat ttccgtcagc aagctgaaga cattcaacgc
     4141 tggcgtaaaa ggctccacgc tgggcgcaag tggcccgcag tacgtgatgg tgccaaagaa
     4201 gcatgcagat caactgcgtg aatctctggc ttcaggcgaa attgctgctg tacagtcgac
     4261 gctggttgcc gacaatacgc cgcttaacag ccgtgtttac accgtacgct ctggcgacac
     4321 gctttcaagt atcgcttcac gtctcggcgt aagcaccaaa gatttgcagc agtggaacaa
     4381 actgcgcgga tctaagctga agccaggcca aagtttgacg attggtgcag gcagtagcgc
     4441 acagcgactg gcaaacaaca gcgatagcat tacgtatcgt gtgcgcaaag gcgattcgct
     4501 ttcaagcatt gctaaacgcc acggcgtgaa catcaaagat gtgatgcgct ggaacagcga
     4561 cactgcgaat ctgcaaccag gcgataagct gacgttgttt gtgaaaaaca acaacatgcc
     4621 agattcctga caaaccagat aataaaaaag gcaccgattc ccccggtgcc ttttttattt
     4681 atgccgcttt atgtgcatct accatgatga tatcactggt gaaaaagcca tcattctgca
     4741 aggcaaaata cgttctcacc tctgtcgatg cgctctgttg gtaaatgcga atagcgtcta
     4801 ctaacgcttc tggcgtacgc attctcgcga cccatgaaga aaattccagc ggtaacttat
     4861 ctgtaattaa attatcaact atcagattgg cttcattgat taacgtcaac cactcaccgc
     4921 tggcgtagtt tcgtacgtga gaggtatcgc gtaatgcttc taccgtctgt aaccagatgt
     4981 cgcgcactgg gtgacccgga gacattacgt ccatcacaat cagcctaccg ccaggtttca
     5041 atatcctatt cacttctcgc agtgctgcac caacatcatg ccagtgatgg gcagaataac
     5101 ggctgataac aatatcaaat gcgttatcgg caaatggcag actttcggca tatccctggc
     5161 gggtggtgat atttttcagt tgccgggctt cggcagcttg tgccacgaca tccagcatat
     5221 gggcagataa gtcatacgcc accaccgtgc tcacgttttg cgcagcgaca aagctggcat
     5281 gccctgctcc gcagcccata tcaagtacac ttgcaccagg ataatcagcc agacgcaccg
     5341 ccaggcgctg taaatctcgt ccgaatgcat gtacggtact ggtcagatat tcgcaggctt
     5401 gagagctaaa ctgcttttct acgtggtcat ggtgggattg tgttgtcatt gcgctgtcct
     5461 tttgttgttt gagtataaaa ggacagcgcc aaacgttggt ctgccctgtg gcagacctga
     5521 cataccttat ttatcaggct tgccgggact gaattcaacg agtagcggat tgtgatcgga
     5581 agcgcgcgta accaatacag aagcttcgct gacgttcaga ccacggtaga aaacaaaatc
     5641 gagcgggcgg ccaaacgccc gacggcgctg atcatcggta aaacgcacct ggcgcagcga
     5701 catttcccgc gcaaagcgat ataacgcgtt catccttcta cggctccagg cattgaaatc
     5761 tcccgccata atgaccgggc cgctgtggtg agctatctga tcaccaatag gaagtaactg
     5821 cttactatag acatccacgc ccagactgaa gttgacggcg tgtatattaa ccaccatcaa
     5881 caggcgggtg tcaggtaatg gatagaccgt caccagtgcc gacttcgcca gacgcaaaat
     5941 gggttctcgt tcacgtaacg ggcagcaata cactggatgc gccgccgaaa gggtcattac
     6001 accggaagga tgttgtggca gcacgaaagc gggtacctga tcggcggcaa gatagttagc
     6061 ggtcgcaaac tgtactaact ctggcgttgt ctgcgcttcc tgcaataaca ccagatgtgc
     6121 atctttgccg tagttcttta ataccgacaa ccattcagcg cgttgctgtt tgtatatgtt
     6181 ccacaccagg atccgaatac gctcttcggt acttaacggt tccccaggtg gtaatgcctg
     6241 gccgatgctc gcaaaagacc ccggcggtaa gatcctttcc gcaggttgtc cggcaacata
     6301 gcgcatggca taggtgtttt ttcgcacttt tgactttgaa acctctatta cgtcaaccct
     6361 tccgcactcg ggagggttct actgttatag ggattttagc tcactctttc aacgagcaat
     6421 tgtatattgt tatgtaagca agtgcttaca ccggtcagaa ttttgctcat tatggagaac
     6481 attaaatcgc atgacatatc acgcatcctg atggtttaat gacaaataaa ttcaaaatat
     6541 ctcttctatt ttgcaacagg agcaaaaatg aaagccacgt cggaagaact cgccattttt
     6601 gtttcggtcg tcgaaagcgg cagctttagc cgggcagcgg aacaattagg gcaagcaaac
     6661 tcagcggtaa gccgggcggt gaaaaagctg gagatgaaac ttggcgttag cctgcttaat
     6721 cggaccacgc gacaacttag cctgacggaa gaaggcgagc gttattttcg tcgcgtacag
     6781 tcaattttgc aggagatggc agcggcagaa tcagaaatta tggagacgcg taatacaccg
     6841 cgtggactgt tacggatcga tgccgcaact ccagtggtgc tgcactttct gatgccgtta
     6901 attaagcctt tccgtgaacg ctatccggaa gtcactttgt cgctagtctc ctccgaaacg
     6961 attattaatt tgatcgaaag aaaagtggat gtcgcgatac gcgctggtac gttaacggat
     7021 tccagcttac gtgccaggcc gttatttaac agttatcgaa aaattatcgc ctcccccgat
     7081 tatatttccc gctacgggaa gccagaaacg atcgacgatt taaagcaaca tgtttgcctg
     7141 ggattcactg aacccgcttc cctcaatacc tggccgatag cctgtagtga tggacaatta
     7201 catgaggtga agtacggttt gtcatccaat agtggggaaa cactgaaaca gctttgcctg
     7261 agtggcaacg ggattgcgtg tttgtccgac tacatgatcg acaaagaaat cgctcgcgga
     7321 gaattggtgg agttaatggc agataaagtg ttgccagtgg aaatgccatt cagtgcagtc
     7381 tattacagcg accgtgcggt aagtacgcgc atccgggctt ttatcgattt ccttagcgag
     7441 catgtaaaaa cagctcccgg aggagctgtc agagaggctt aatcccattc aggagccaga
     7501 ccttccgggc taaccaggcg gtcgttgcaa tccagtgcgg cgatcgcttt tttatcttcg
     7561 gcatcaagct gtaaattttg tgccttaaga ttactttcca ggtttttacg tttagtagaa
     7621 gaaggaatta ctgagtaacc ttcccccata gcccacgcca gaatcacttg tgccggagtc
     7681 gcattgtgtt tagctgcaat acgagcaata acctcatctt tcagtgcctt accataagcc
     7741 agcgtcatat aggaagtgat atgaataccg tgctgtttag cccaggcaac cactttacgg
     7801 ttttgcagat aaggagagag ttcaatctgg ttggtagcga tgttttcagc gccaacagca
     7861 gcaatcgctt tttccatcaa tgggatcgtg aagttggaaa taccgatctc acgcgtcagc
     7921 ccttgttttt tggcttccag cagcgcctgc ataaactctt caacagagac ttcatcgttt
     7981 ggtgacggcc agtggattag cgtcagatca acataatcgg tacgcaattt ttgcaggctc
     8041 tctttcagac tcgggatcaa tttgtctttg ctgagatttt caatccagat tttagtggtg
     8101 atgtagagtt catgacgtgg tacgccactt tctgcaatcg cctgacctac tgcggcttcg
     8161 ttatcataga tttgtgcggt atcaattgcg cgataaccaa gttcaagcgc cgttttcaca
     8221 gatgaaataa caacgtcgtc tttcagacgg aaagtaccta aaccaaatgc agggatagcc
     8281 ataatatgcc tcttttaatg ctattttcgt taactgaggt gattatgggc ttcagactgt
     8341 tgcggaaaaa gagcgtaaaa cgcagaggat ttttgcgatt ctagcaataa tagatatcca
     8401 gacgaaaaaa aacctcgatc gttaactcga ggcttcttaa t
//
LOCUS       DAOFQD010000173         8227 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.173, whole genome shotgun sequence.
ACCESSION   DAOFQD010000173 DAOFQD010000000
VERSION     DAOFQD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.173"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1374
                     /locus_tag="QBF46_004323"
     CDS             <1..1374
                     /locus_tag="QBF46_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HDQ6676431.1"
                     /translation="FVIKFSTSVLQADKKEITIINKNENTTLTQTIAPIFEEYLMEIL
                     PQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAAQYINDIYQN
                     VDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSRPEGRCVAACGDFGLVMKAYFDKM
                     ESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSESKED
                     IKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDEITPPLSPAT
                     MNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNVINTIFNALPTLFNTFK
                     FDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSNHARLFGFTAEDIMDFW
                     QHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTDNPRYIAEKNYMEALLKK
                     ASPHTVR"
     gene            complement(1343..3079)
                     /gene="aceK"
                     /locus_tag="QBF46_004324"
     CDS             complement(1343..3079)
                     /gene="aceK"
                     /locus_tag="QBF46_004324"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDQ6676432.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLHVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDINYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3360..4664)
                     /gene="aceA"
                     /locus_tag="QBF46_004325"
     CDS             complement(3360..4664)
                     /gene="aceA"
                     /locus_tag="QBF46_004325"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDQ6676433.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4694..6295)
                     /gene="aceB"
                     /locus_tag="QBF46_004326"
     CDS             complement(4694..6295)
                     /gene="aceB"
                     /locus_tag="QBF46_004326"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDQ6676434.1"
                     /translation="MTEQVTTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6564..7493)
                     /gene="metA"
                     /locus_tag="QBF46_004327"
     CDS             complement(6564..7493)
                     /gene="metA"
                     /locus_tag="QBF46_004327"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDQ6676435.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQDQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7650..8093
                     /locus_tag="QBF46_004328"
                     /pseudo
     CDS             7650..8093
                     /locus_tag="QBF46_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         2097 a         2051 c         2030 g         2049 t
ORIGIN      
        1 tttgtcatta aattttccac gtccgtctta caagctgata agaaagaaat tacgataatt
       61 aataaaaacg aaaacacgac acttactcaa accattgccc caatatttga agaataccta
      121 atggaaattt tacctcaacg ctcagacact cttgataaac aagaattaaa cctaaaatca
      181 gatagaaaag aaaaagaatt cccaagaatt aaacttaatg gtcaatgtta ttttccgggg
      241 cgaccccaaa accgtatagt atgccgacac attgctgcac aatatattaa tgatatttat
      301 cagaatgttg attacaaacc ccatcaagat gattactctt cagctgaaaa atttctcacg
      361 cacttcaaca aaaaatgcaa aaaccagact ttggcgttgg tttccagccg tcctgagggg
      421 cgttgcgttg ctgcctgcgg tgatttcggg ctagttatga aagcatattt tgacaagatg
      481 gaatcaaatg gcatcagtgt tatggcagcc atattactgg tggataacca tgctttgacg
      541 gtccggctaa gaataaagaa cacaactgaa ggatgtaccc attacgtggt ttcggtttat
      601 gatcctaatg taactaacga taaaataaga attatgagcg aaagcaaaga ggatattaaa
      661 cactattctc tgatggattt tatgaatgta gattatagcc tcctgaaatg gtcaaatgat
      721 catgttatta accaatctgt tgcaataatt ccagcacttc cgaaagaaca gctattgatg
      781 ttaaaaggat ctgtggatga aataacccct ccattatcac cagcaacgat gaatttgcta
      841 atggcaattg gtcagaatca ccaacttacg caactgatga ttcagctcca gaaaatgcca
      901 gaactacata gaacagaaat gttgactgcc tataatagtg gacatatgaa cgttattaat
      961 actattttta acgcattacc cactctgttt aatacgttta aattcgataa aaaaaatatg
     1021 aagcccctcc tcctggcaaa taattctaat gaatatcccg gtttgttttc agcgatacag
     1081 cataaacaac aaaatgttgt agagacggtt tatcttgctt tatctaacca tgcacgcctg
     1141 tttggattta ccgctgaaga tattatggat ttttggcaac acaaagcccc acaaaaatac
     1201 tctgcctttg agttggcttt tgaattgggt caccgggtta ttgctgaatt aatccttaat
     1261 acattaaata agatggctga aagctttggc tttacggata accctcgata cattgcggag
     1321 aaaaattata tggaagcttt actcaaaaaa gcatctcccc ataccgtacg ctaaatcttt
     1381 gcctgcgccg atacgcataa acatcttcca catgcccttc acgtatgcgg ttttgtagtg
     1441 cgcgccagta atcagcgcgg aacaggtcgg cgtgcatctc ttcaaacagc ggaccaatac
     1501 gcgggtcggc gcatagccag tggcgaaact cttccgggaa aacatcgccc ggcgagacgc
     1561 tgtaccacgg ttcgctggca agttcgtctt ccggatagcg cggcggcggg atgtcgcgga
     1621 aattcacttc cgtcatgtag caaatttcat cgtaatcata aaaaaccaca cgcccgtgac
     1681 gggtgacacc gaagttttta aacagcatgt cgccaggaaa aatgttagca gcggcaagct
     1741 ggcgaatagc gttaccgtat tcttcaatgg cgtcgcgcaa ctgctgacct tccacttgtt
     1801 ccagccagat attgagcggc accatccgcc gctcgatata gagatggcga attacaatct
     1861 gctcaccaag atcggtgatt ttttccgctg cttcctggag cagtaattcc attaatgccg
     1921 gggaaatatg ccgcttctcc agcacaaagt tttcaaactc ctgggtgtcc gccattcggc
     1981 ccacacggtc gtgctctttc accagttgat agcaggcacg aacgtgagcg gcagacatct
     2041 ctttctgcgg cgcgaacttg tctttgatga ctttgaatac ccgatcaaag cccggcagcg
     2101 taaataccaa catcaccatt ccacgaatac ccggcgcttc aatgaactgc tcattacagc
     2161 cctgtagata aacgagatat tcgcggtagc tttcggtttt ggcgtgcttc tggcagccga
     2221 tagccatata caattcagca gtggttttac ctggcagaat ttcccgtagc cactcgacca
     2281 gcgctgcggg cagcggtgca taaaccataa aataagaacg cgcaaagcca aaaacaatgc
     2341 tcgcttcggc ggtcgtcgtc aggcaggtat caataaataa ctcgccgtcg tccgtctggt
     2401 ggatcggcag caaaaatggc aatgtgccgg aaggcgtgat cagtttgcct accagccagg
     2461 cggctttatt gcggtaaaac agttcgttcg ccacctgtaa atgactttcc gcgaggttgt
     2521 ctgtccccag cgtttccgtc agatggcgaa taatgtaatt gatatcacgg cttttattct
     2581 gccagcgcag gcgcagcggc aggtcgctga taacgcgcat cagcagagat tcccagccgt
     2641 gatcggggtg aaagtctttc gccagcgggc ggggaatggt acgaaagcgg cgctctggct
     2701 gggagctaaa gataaaaagc cgctcgggag taagcgagcg gtggtcaaat aaccgacagt
     2761 acacggagtt aaaaaagctc tccgcaatct cgaagcgcgg gtaatccggc aacagccgcg
     2821 tgtaatgctc tttaacgtgc agtaaaaatg ccgcgtccgt actttggccg ttagtaatgc
     2881 agcgcagttg ctccacgacc agaccaacgt gatgatcgta aagatggata cggtttttca
     2941 tcgcctgctg gacagcatgc cagtcggcct gttcgaaacg ctgctgcgcc ccggaagtca
     3001 cttcgaggaa tcgaccatac tgagcgtcga agccttgcaa aatggtttgt gcaatcagta
     3061 attccaggcc acgcggcatt tagcgccctc atcaggagca gagaattgcc ggatgcggcg
     3121 tgaacgcctt atccagccta cgttcggcaa cggttgtagg catgataaga cgcgcaagcg
     3181 tcgcatcagg catcgtgcac cgattgccgg atgcggcgta aatgccttat ccggcctacg
     3241 ttcggcaacg gctgtaggca tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg
     3301 attgccggat gcggcgtgaa cgccttatcc ggcctacagt cagcaacggt tgttgttgct
     3361 tagaactgcg attcttcagt ggagccggtc agcgcggtga ctgaagacgt gccgccctga
     3421 ataatagtcg tcactttatc gaagtaacct gtacccactt cctgctggtg agatacgaag
     3481 gtatagccat ctttcgcggc ggcaaattcc ggctgctgca ctttctcaac gtagtgcttc
     3541 ataccctcgc cctgggcata ggcgtttgcc aggtcaaaca tgttgaacca catgctgtgg
     3601 atacctgcca gggtgatgaa ctggaacttg tagcccatat ccgacagctg ctgctggaag
     3661 ctggcaatag ttttgtcgtc gaggtttttc tgccagttga acgacggcga gcagttataa
     3721 gccagcagtt tgcccggata tttcgcgtgg atagcttgtg caaagcgacg cgccagttcc
     3781 agatccggcg tggaggtttc acaccagacc aggtcagcat atggcgcata cgccaggcca
     3841 cggctgatcg cttgctcaat gcccgcatga gtacggaaga agccttcact ggtacgctcg
     3901 ccagtaataa attcactgtc atacgggtcg caatcggagg taatcagatc cgccgcatcg
     3961 gcatcggtac gagcaaccag cagggttgga acgcccgtca cgtcagctgc aagacgcgcc
     4021 gcgaccagtt tctgaatagc ttcctgagtt ggcaccaaaa ctttgccgcc catgtgaccg
     4081 catttcttca ctgacgccag ctgatcttcg aagtgaactg ccgctgcacc ggcttcaatc
     4141 atcgctttca tcagttcaaa ggcattcagg acaccgccaa aaccggcttc cgcatcggca
     4201 acgatcggca ggaagtaatc gacatagcgc ggatcgcccg gctcaatgcc tgcggaccat
     4261 tggatctgat cggcacgacg gaaggtgttg ttgatccgct ccaccacagc tggcaccgag
     4321 tttgccggat agagcgactg atccggatac atgctggccg ccaggttagc gtccgccgct
     4381 acctgccatc ccgacagata gactgcttca atacccgctt tcgcctgttg cagcgcctga
     4441 ccgccagtca gtgcaccgag gctgttgatg tagccttttt tcgactcgcc atgcagcaga
     4501 cgccacattt tcgccgcgcc cagttgcgcc agggtgcatt caggattgac tgaaccgcgt
     4561 aatttcacca catcttccgc gctgtatggg cgagtaatgc cttcccaacg cggttgagtc
     4621 cactctttct gtaattcttc aatttgttgt gtacgggttt tcatgtgcag atgctccata
     4681 gttatgtggt ggtttacgct aacaggcggt agcctggcag ggtcaggaaa tcaattaact
     4741 catcggaagt ggtgatctgt tccatcaagc gtgcggcatc gtcaaaacgc ccctgggaga
     4801 aacgttcttc gcccagttcg ctggcaatga ctttcatctc ttcacccagc atctggcgga
     4861 acaaggcttt ggtcaccggt ttgccattgc tcaacgtttt ttgatgatgg atccactgcc
     4921 agattgaggt acgggaaatt tcagccgtcg ccgcatcttc catcaggcca taaatcggca
     4981 cgcagccgtt gccggagatc catgcttcga tgtactgcac tgccacgcga atgttggcgc
     5041 gcataccttc ttcggtgcgt tcaccatcac aaggtgccag cagctgatcg gcagtaatcg
     5101 gcgcgtcttg ttcgcgcatc acttcaagct gatttttacg ggagccgaga atgtcgttga
     5161 ataccgccat tgccgtatcc gcaaggcctg ggtgagcaat ccatgtgcca tcgtgaccgt
     5221 tattggcttc cagcgcttta tccgctttta ctttgttgag cacctggtta ttgcgctctt
     5281 catctttgct cggaataaac gctgccatgc cgcccatcgc aaaagcaccg cgtttatggc
     5341 aggttttaat caacaggcgt gagtaagcat tcaggaatgg tttatccatc gtcactgcct
     5401 gtctgtctgg caggacgcga tcgggatagt ttttcaacgt tttgatatag ctgaagatgt
     5461 aatcccaacg accgcagttc agaccaacaa tatggtcacg cagcgcgtga aggatttcat
     5521 ccatctggaa cacggcgggc agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg
     5581 gcagattaaa gcgatcttct gcatagctga agacttcgct ccaccaggcc gcttcctgcc
     5641 aggactgggt tttcggcaga tagaaatagg gaccgctgcc ctttgccaac agtgcctgat
     5701 agttgtggaa gaagtagagc gcaaaatcaa acaggctacc ggggattgcc tcaccacgcc
     5761 aggtgacatg tttttccggc aagtgcagac cgcgtacccg acaaatcaaa accgctggat
     5821 tgggcttgag ctggtaaatt ttgcctgctt cattggtgta actgatggtg ccgttaaccg
     5881 catcgcgcag gttaatttgc ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt
     5941 cgaaatcggc cataaagact ttcacattgg cgttcagtgc gttgatcacc atcttgcgct
     6001 caaccgggcc agttatctcg actcgacgat cttgtaagtc cgcgggaata ccacgaattt
     6061 tccagtctgt atcgcgaatg gaagctgttt ccgaaataaa atcaggcaac gttccgttat
     6121 caatatcttg ctgctgctga atacgcgccg ccagaagttt attgcgttgt ggcgtaaaat
     6181 gcgtcaccag ctcagtcaga aattctaccg cttcggcagt aagaatttgc ttctcctgct
     6241 cgccatacgg ccttgtgaaa gccagttcat cggttgttgt tacctgttca gtcatcgtgc
     6301 agctcctcgt catggatccg aaaacttccc cactgtgaac gaaggatcgt tgtgcgcttt
     6361 tcgttcagca caactaagac tactcattta aaatgcaaaa tcaaaaacaa tttccatttt
     6421 aattaaaaat acttgataac ttattgataa caatatgaat aaaatttatt cacctcctga
     6481 ggtgcgtttc ggaaataaaa aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct
     6541 taacgatcga ctatcacaga agattaatcc agcgttggat tcatgtgccg tagatcgtat
     6601 ggcgtgatct gatagacgta atagttgagc cagttggtaa acagcaaatt accgtgacta
     6661 cgccagctcg ctcgcggttt gttttgcgga tcattgtgcg ggaaatagtt atacggtaca
     6721 tccgggtcta gtccggcttc cacatcgcgg aaatattcct gcgccagcgt ttgcgcatca
     6781 tattcgggat ggcccgtcac aaaggcaatt cgcttatctt tgctggcaaa cagatatgca
     6841 tccccttctt ccgtctctgc cagaatttcc agatcggtgt aatcacgaat caacgctgcc
     6901 ggaaagtcag catagcgcga atgcggtgcc aggaatgaat catcaaagcc acgcgtcaga
     6961 agcgcatgag gatggagaat atgatgctcg taaacgccag agagtttgtc ggtgcgagtt
     7021 tgcttaggaa tgccgtagag gatattgagc gcggcctgta ccgcccagca gacaaacagc
     7081 gtcgaggtga cgtgatcttt cgaccactcc agcacctgtt tgatctgcgg ccagtaagcg
     7141 acatcattaa actccaccag gcccagcggc gcaccagtta caatcaaacc gtcaaagttc
     7201 tgatcctgaa tatcttcaaa gttacagtag aagttgttca gatgctctgc gggcgtgttg
     7261 cgcgattcac gggaatcgat gcgcaacagc tgaatatcga cctgcaaagg tgagtttgaa
     7321 agcaggcgca gaaactgatt ttcagtttca atcttcttcg gcatcaggtt aaggatcaga
     7381 accttaagtg gacgaatttc ctgaccagac gcacgagaag ttgtcatcac aaagacgttt
     7441 tcttcacgca agaaattgac ggcgggtagc tcgtccggca cacgaatcgg cataacctga
     7501 ttacctcact acatacgctt atacgtttag acatccagat agctgaagat aaccagaaaa
     7561 tttgccaatg tcgagcctgc atgttgaagg tgagaaagtt tcaaggaaaa gactacgtta
     7621 gaatataaga atgactaaaa ggagagaaaa tggttattag tattcgccgc tcacggcatg
     7681 aggaagggga ggaactcgtt gcgatttggt gtcgttctgt cgatgccact cacgattttc
     7741 tatcagcaga gtatcggacc gagctggagg acctagttcg ttccttcctg ccggaagcgc
     7801 cgttgtgggt cgcggttaat gagcgggatc agccggttgg atttatgttg ctaagtgggc
     7861 agcatatgga tgcgctgttt atcgatcctg atgtgcgcgg ctgcggcgta ggtcgggtgc
     7921 tggtggagca tgcgctctcg atggcaccgg aactgacaac caacgttaat gagtaaaatg
     7981 agcaggcggt tgggttctat aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg
     8041 acgatttggg gaaaccgtat ccgttgctga atctggcgta tgtgggggaa taagccgccc
     8101 ctgccgtgcc tgcccaggct ggagaagacg cgccagcatc gcacccggca tttttttcac
     8161 caacgcaaaa aggccatcct gacggatggc ctttcgcttg atttgatgcc tggcagttcc
     8221 ctactct
//
LOCUS       DAOFQD010000174         8106 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.174, whole genome shotgun sequence.
ACCESSION   DAOFQD010000174 DAOFQD010000000
VERSION     DAOFQD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.174"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..124
                     /gene="lar"
                     /locus_tag="QBF46_004329"
     CDS             <1..124
                     /gene="lar"
                     /locus_tag="QBF46_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="seems to be involved in restriction alleviation and
                     enhancement of modification by EcoKI, but not in
                     restriction alleviation of type III restriction systems;
                     Rac prophage; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDQ6676436.1"
                     /translation="YYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV"
     gene            117..326
                     /gene="rcbA"
                     /locus_tag="QBF46_004330"
     CDS             117..326
                     /gene="rcbA"
                     /locus_tag="QBF46_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HDQ6676437.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSSRIALYSN"
     gene            405..620
                     /gene="xisR"
                     /locus_tag="QBF46_004331"
     CDS             405..620
                     /gene="xisR"
                     /locus_tag="QBF46_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HDQ6676438.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            622..1857
                     /locus_tag="QBF46_004332"
     CDS             622..1857
                     /locus_tag="QBF46_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6676439.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            1909..2844
                     /gene="ttcA"
                     /locus_tag="QBF46_004333"
     CDS             1909..2844
                     /gene="ttcA"
                     /locus_tag="QBF46_004333"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDQ6676440.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2973..4346)
                     /gene="dbpA"
                     /locus_tag="QBF46_004334"
     CDS             complement(2973..4346)
                     /gene="dbpA"
                     /locus_tag="QBF46_004334"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDQ6676441.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4824..5807)
                     /gene="zntB"
                     /locus_tag="QBF46_004335"
     CDS             complement(4824..5807)
                     /gene="zntB"
                     /locus_tag="QBF46_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDQ6676442.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            6062..7294
                     /gene="dgcM"
                     /locus_tag="QBF46_004336"
     CDS             6062..7294
                     /gene="dgcM"
                     /locus_tag="QBF46_004336"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDQ6676443.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMFQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFRRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7315..7878)
                     /gene="smrA"
                     /locus_tag="QBF46_004337"
     CDS             complement(7315..7878)
                     /gene="smrA"
                     /locus_tag="QBF46_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDQ6676444.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         2297 a         1950 c         1911 g         1948 t
ORIGIN      
        1 atattaccga gcgaagtgta acggatgcga atcccgaacc ggttatggtg gaagtgaaaa
       61 agaagcactc gaaagatgga ataaacgaac cactggaaat aataatggag gtgttcatgt
      121 ataaaattac cgccactatt gaaaaggaag gtggcactcc tactaactgg acaagatatt
      181 caaaatctaa actaacgaaa tcagaatgcg aaaaaatgct ctcaggtaaa aaagaagcag
      241 gcgtttccag agagcagaaa gtaaaattga taaattttaa ttgcgagaaa cttcagtcct
      301 cgagaattgc attgtattca aattaaaact tcatagctga ttattaataa tcaacatcgg
      361 gcgtcaattt cagtctaaca ttggcgcctg ccagaggtga tgcgatggca caagtaatct
      421 ttaatgaaga gtggatggtt gaatacggcc tgatgcttcg cactggtctg ggggccagac
      481 aaattgaagc ataccgccag aactgttggg tggagggctt ccacttcaaa cgagtatctc
      541 ctttaggtaa gccagacagc aaacgaggga ttatctggta caactatcca aagataaatc
      601 agtttatcaa agactcatga tatgtctaaa ttaccaacag gtgtcgagat tagaggtaga
      661 tacattcgca tctggttcat gtttcgagga aaacgatgtc gggaaacatt aaaaggctgg
      721 gagattacaa acagtaatat taaaaaggcc ggaaatttaa gagcgctgat agttcatgaa
      781 ataaactccg gtgaatttga gtatttaaga cgttttcccc agtccagcac tggggcaaaa
      841 atggtgacaa cgagagtcat aaaaacgttc ggagagcttt gtgatatctg gacaaaaatt
      901 aaagagacag agttaacaac aaacacaatg aagaaaacga aatcacaatt aaaaacactc
      961 agaataataa tttgtgaaag taccccgata tcacatattc gttatagcga tatcttaaac
     1021 taccggaatg aactgctgca tggagaaacg ctttacctgg ataatccaag atccaacaaa
     1081 aaaggaagaa ccgtgcgcac agttgataac tatatcgccc tgctctgttc gctgttgcgt
     1141 tttgcgtatc agtcgggatt tatatcaacc aaaccatttg aaggagtaaa aaaattacag
     1201 cgaaacagaa taaagcctga tccgttatct aaaacagaat tcaatgcatt aatggaaagt
     1261 gaaaaaggac agagccagaa cttgtggaaa tttgccgttt actcaggact tcgtcacggg
     1321 gaactggcag ctctggcgtg ggaggatgtg gatctcgaaa agggaatagt gaatgtcaga
     1381 agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg gatccgaaca
     1441 gtaacactac tgcagcctgc tcttgaagca ctgaaggagc aatacaaact gaccgggcat
     1501 catcgcaaaa gcgaaatcac cttttatcat cgggagtacg gcagaaccga aaagcaaaaa
     1561 ctgcattttg ttttcatgcc cagggtgtgt aacggaaaac aaaaacctta ttactcggta
     1621 agcagtttgg gggcaaggtg gaatgcagca gtaaaacgtg ctggtattcg ccgccgtaat
     1681 ccgtaccata cgcggcatac ttttgcctgc tggctgttga cggcaggagc gaacccggca
     1741 tttatagcca gccaaatggg gcatgaaact gcgcagatgg tgtatgaaat ttacggtatg
     1801 tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt atcgtagttg
     1861 caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat gcaagaaaat
     1921 caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg tctgcgtcgt
     1981 aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg catcatggtt
     2041 tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa tttgcagcaa
     2101 agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca accgggcttc
     2161 ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa gattgttgaa
     2221 gagaatactt acggtatcgt gaaagagaag attccagagg gcaaaaccac ttgctcactg
     2281 tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg ggcgacgaag
     2341 atcgcgttgg gtcaccatcg tgacgatatc ctgcaaacgt tgttcttaaa tatgttctac
     2401 ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa acatatcgtt
     2461 attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga tgcaaaagcg
     2521 ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg tcaggtgatt
     2581 gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac gatgttcagc
     2641 gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga tttcaaaggc
     2701 attacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg cgaagagatc
     2761 ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga tgagttacgg
     2821 ctgaatgtgg ttgaagtgaa ataaccagga tagcgcccga tgcgcaagcg tatcgggcta
     2881 ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatgcc gaagaagatg
     2941 tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac ccggcacgtt
     3001 tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac agcctgacgg
     3061 accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc gccatcaagc
     3121 ccgatatctc ctgtcagcgc acctaataca tcacccgggc gcattttggc ttttttccca
     3181 ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact attagctggc
     3241 ggcgtttgcc agtttagttt tatctgcaac atgtcagaaa tgatattggc ccgctgtgct
     3301 tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgcgc tgtacgaccg
     3361 attcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac cagctcaagc
     3421 gatttaatat ccagaccacg cgcagcaaca tcagtcgcga ccagtacacg ggcgctaccg
     3481 ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc gtgtaatgac
     3541 aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc ttttttggta
     3601 ttgcaaaaca ccacgcaaga agatggctga tgcaagctta ataaccgttg caacagagga
     3661 attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa agcatctgtt
     3721 gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattgc agcgatggct
     3781 tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg ctggcgcaaa acggatgaca
     3841 tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc catcaccagc
     3901 gtattcaacg catccagtga taccgtgcct ttttgcaggt gatccagcaa acgccccggc
     3961 gttgccacaa taatatgcgg cgcatgttgc aacgaatcac gctgcatacc gaacggttga
     4021 ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg acgcaattca
     4081 cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg ggtttgaaat
     4141 agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt gccgctgcca
     4201 gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc ggcggcctgc
     4261 accggcgtca tggttaaata acccaactca ttaaggttcg tgagttgggc gggaggcaaa
     4321 acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg caaagttaac
     4381 aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt tcttcgtcgg
     4441 gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga acaggatcgc
     4501 caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata tttccctccg
     4561 gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa aaaagccgac
     4621 tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa aaaggaagta
     4681 agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa gggagatatt
     4741 gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt tttgtataca
     4801 gaaagccgga aaaattgctt ttgttacaac catttactac gatgcaacca taaagcaaca
     4861 ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg ccacccgccg
     4921 ccagggatcc caccaaggtt gacgccaaat aaccctgtca gaaaggtact gggtaaaaag
     4981 accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg catcacctga
     5041 gcgatttcat ccgccatcac accagtccgt gctatacagg cgtcgatttc gtcaaggccg
     5101 cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct catccacggc
     5161 aaacgttcac tggcaagacg agcatagaca tcacgttgcg gtgccatata gcgacgcatc
     5221 acgattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg ctgatcaagg
     5281 agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc gctggaatga
     5341 tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg acccgtgccc
     5401 tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg ggtcgaaaca
     5461 attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg ttcatcggtg
     5521 ctgccgttta tacagcgcaa tgtaataagc gtgccttcac cgagacggct gactcggggc
     5581 cgtgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg tgttgtcgcc
     5641 agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg atgcgcttca
     5701 tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag catccaggca
     5761 aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt gcctccatca
     5821 tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct catcacccca
     5881 ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca aaatgctttt
     5941 ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat tatctgatca
     6001 tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata atgggatctc
     6061 aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct ggatcgtttc
     6121 gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga tgcaagacct
     6181 cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag aactgccgaa
     6241 atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg ttcagcgtaa
     6301 agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag aaacagaacc
     6361 ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg gggttaaaag ccagtcgctc
     6421 ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga ctaactctgc
     6481 accaatgttg ttgattgacc cgtcacgaga tggacaaatc gtcgatgcta acctcgccgc
     6541 gctcaatttc tatggttata accatgaaac gatgttccag aaacatacct gggaaataaa
     6601 tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac ccggcggtca
     6661 taaacctttg aattttgttc ataaactggc ggatggatct actcgtcatg tgcagaccta
     6721 tgccggaccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc atgatattac
     6781 tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg cgatgaccgg
     6841 attactgaat cggcgacagt tttatcacat tacggaacca ggccaaatgc agcatctcgc
     6901 catcgctcag gattacagct tgttgctcat cgacaccgat cgttttaaac acattaacga
     6961 tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca ccctcgaaag
     7021 ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg tcttattgct
     7081 accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg taagcgtggc
     7141 aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg cgcatcacga
     7201 aggaaatgaa agcatcgatg aactgtttag acgcgttgat gatgctttgt atcgggcgaa
     7261 aaatgatggt cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc gagatcaacg
     7321 actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt tacgtagtgc
     7381 gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg cctgaacatc
     7441 atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat gcgatttatc
     7501 atcccgccct ttaccatgaa taatcagcac gttacgcaaa ccatccgcca gggcttgttg
     7561 aataaaactg aacaccattt tgcggcactc ttccaccggc tggcgcaaaa gattcaggct
     7621 cgcctgttgc ggatatttac cactacgcag cttatccagc accccatgtt gcaacccttc
     7681 ccgccgaaac tccagcggct gacttagcgg gataatgtcg agaaatccgg tagtgaggaa
     7741 attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg ttggatgcca
     7801 gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa gaaacagcga
     7861 tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat gataaccaga
     7921 ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa atattgccga
     7981 tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa tgtaacttat
     8041 attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac cgttataaca
     8101 cagtca
//
LOCUS       DAOFQD010000175         8084 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.175, whole genome shotgun sequence.
ACCESSION   DAOFQD010000175 DAOFQD010000000
VERSION     DAOFQD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.175"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            260..763
                     /gene="dps"
                     /locus_tag="QBF46_004338"
                     /gene_synonym="pexB"
     CDS             260..763
                     /gene="dps"
                     /locus_tag="QBF46_004338"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDQ6676445.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1167..1913
                     /gene="glnH"
                     /locus_tag="QBF46_004339"
     CDS             1167..1913
                     /gene="glnH"
                     /locus_tag="QBF46_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDQ6676446.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2052..2711
                     /gene="glnP"
                     /locus_tag="QBF46_004340"
     CDS             2052..2711
                     /gene="glnP"
                     /locus_tag="QBF46_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDQ6676447.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2708..3430
                     /gene="glnQ"
                     /locus_tag="QBF46_004341"
     CDS             2708..3430
                     /gene="glnQ"
                     /locus_tag="QBF46_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDQ6676448.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3691..5916
                     /gene="ybiO"
                     /locus_tag="QBF46_004342"
     CDS             3691..5916
                     /gene="ybiO"
                     /locus_tag="QBF46_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDQ6676449.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(5913..6839)
                     /gene="rlmF"
                     /locus_tag="QBF46_004343"
     CDS             complement(5913..6839)
                     /gene="rlmF"
                     /locus_tag="QBF46_004343"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDQ6676450.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7115..7375
                     /locus_tag="QBF46_004344"
     CDS             7115..7375
                     /locus_tag="QBF46_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ6676451.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSSQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7640..>8084
                     /locus_tag="QBF46_004345"
     CDS             7640..>8084
                     /locus_tag="QBF46_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDQ6676452.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTE"
BASE COUNT         1965 a         2065 c         2019 g         2035 t
ORIGIN      
        1 ttctcgctac ttttcctcta caccgtcttt atatatcgaa ttatgcaaaa gcatatttat
       61 tccgaaaatt cctggcgagc agataaataa gaattgttct tatcaatata tctaactcat
      121 tgaatcttta ttagttttgt ttttcacgct tgttaccact attagtgtga taggaacagc
      181 cagaatagcg gaacacatag cctgtgctat acttagtctc gttaattact gggacataac
      241 atcaagagga tatgaaatta tgagtaccgc taaattagtt aaatcaaaag cgaccaatct
      301 gctttatacc cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag agttgctgaa
      361 tcgccaggtt atccagttta ttgatctttc tttgattacc aaacaagcgc actggaacat
      421 gcgcggcgct aacttcattg ccgtacatga aatgctggat ggcttccgca ccgcactgat
      481 cgatcatctg gataccatgg cagaacgtgc agtgcagctg ggcggtgtag ctctggggac
      541 cactcaagtt atcaacagca aaaccccgct gaaaagttac ccgctggaca tccacaacgt
      601 tcaggatcac ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg acgtacgcaa
      661 agcgattggc gaagcgaaag atgacgacac cgcagatatc ctgaccgccg cgtctcgcga
      721 cctggataaa ttcctgtggt ttatcgagtc taacatcgaa taaatccatc gctgatggtg
      781 cagaacttta gtacccgata aaagcggctt cctgacagga ggccgttttg ttttgcagcc
      841 cacctcaacg cacttattta gtgcatccat ctgctatctc cagctgatta agtaaatttt
      901 ttgtatccac atcatcacac aatcgttaca taaagattgt tttttcatca ggttttacgc
      961 taaataatca ctgtgttgag tgcacaattt tagcgcacca gattagtgcc ccagaatggt
     1021 gcatcttcag ggtattgccc tataaatcgt gcatcacgtt tttgccgcat ctcgaaaaat
     1081 caaggagttg caaaactggc acgatttttt catatatgtg aatgtcacgc aggggatcgt
     1141 cccgtggata gaaaaaagga aatgctatga agtctgtatt aaaagtttca ctggctgcac
     1201 tgaccctggc ttttgcggtt tcttctcatg ccgcggataa aaaattagtt gtcgcgacgg
     1261 ataccgcctt cgttccgttt gaatttaaac agggcgataa atatgtgggc tttgacgttg
     1321 atctgtgggc tgccatcgct aaagagctga agctggatta cgaactgaag ccgatggatt
     1381 tcagtgggat cattccggca ctgcaaacca aaaacgtcga tctggcgctg gcgggcatta
     1441 ccatcaccga cgagcgtaaa aaagcgatcg atttctctga tggctactac aaaagcggcc
     1501 tgttagtgat ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat ctcgacggga
     1561 aagtggttgc tgtgaagagc ggtactggct ccgttgatta cgcgaaagca aacatcaaaa
     1621 ctaaagatct gcgtcagttc ccgaacatcg ataacgccta tatggaactg ggcaccaacc
     1681 gcgcagacgc cgttctgcac gatacgccaa acattctgta cttcatcaaa accgccggta
     1741 acggtcagtt caaagcggta ggtgactctc tggaagcgca gcaatacggt attgcgttcc
     1801 cgaaaggtag cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc ctgcgcgaga
     1861 acggaactta caacgaaatc tacaaaaaat ggttcggtac tgaaccgaaa taataacgct
     1921 acacctgtaa agcgcactgg cagttccctc tcccctctgg ggagaggatt agggtgaggg
     1981 gcgcaaaccc gctccggggc cattaattac cctgaatttg attatttaca ccacggtaac
     2041 aggaacaaca tatgcagttt gactggagtg ccatctggcc tgccattccg cttctgattg
     2101 aaggtgccaa aatgaccctg tggatttcgg tcctcggtct ggctggcggt ctggtaatcg
     2161 gattgctggc aggttttgca cgcaccttcg gtggttggat agccaaccac gtcgcgctgg
     2221 tctttattga agtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt atctatttcg
     2281 ccctgccgat ggcgtttaac gacttacgca tcgacccatt tactgcggcg gtggtcacca
     2341 tcatgatcaa ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg ctgtctatcc
     2401 acaaaggttt tcgtgaagca ggactggcgc tcggtctttc acgttgggaa accattcgct
     2461 acgtcatttt accgctggca ctgcgtcgta tgctgccgcc gctgggtaac cagtggatca
     2521 tcagcattaa agacacctcg ctgtttattg tgatcggcgt ggcggaactg acccgtcagg
     2581 ggcaagaaat tattgccggt aacttccgcg cccttgagat ctggagcgcc gtggcggtgt
     2641 tctatctgat tattaccctg gtgctgagct ttattctgcg tcgtctggaa agaaggatga
     2701 aaatcctgtg attgaattta aaaacgtctc caagcacttt ggcccaaccc aggtgctgca
     2761 caatatcgat ttgaacattg cccagggcga agtcgtggtg attatcgggc cgtccggttc
     2821 cggtaaatcg accctgctgc gctgcatcaa caaactggaa gaaatcacct ccggcgatct
     2881 gattgtcgat ggcctgaagg ttaacgatcc gaaagttgac gagcgcctga ttcgccagga
     2941 agcaggtatg gtgttccagc agttttacct cttcccgcat ctgacggcgc tggaaaacgt
     3001 catgtttggc ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa aactggcgcg
     3061 tgagctgctg gcgaaagtcg gtctggcaga acgtgcacat cactaccctt ccgaactttc
     3121 tggtggtcaa cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc cgaaaatgat
     3181 gctgtttgat gaaccgactt ccgctcttga cccggaactg cgccatgaag tgctgaaggt
     3241 tatgcaggat ctggctgaag aagggatgac gatggtgatc gtgacccacg aaatcggttt
     3301 tgccgagaaa gtagcttcgc ggctgatctt tatcgacaaa ggccggattg cggaagatgg
     3361 cgatccgcag gtgttgatca agaacccgcc gagccagcgc ttgcaggaat ttttgcagca
     3421 cgtctcttaa taagacacat tgcctgatgc gctacgctta tcaggcctac aggatatctg
     3481 gcaacttatt aaaattgcat gaacttgtag gacggataag gcgttcacgc cgcatccggc
     3541 aaaaaagccc gcacgttgtc agcaacctgc ttaatatccc ttcctccctt tcacccgaaa
     3601 gggaggcaca ccagattcct ctcatttaaa atcgcccctc cctcagcatc tatacttatc
     3661 tttttgctct gttttctcac tggaggagtc atgcggtgga tcctgttcat cctcttctgc
     3721 ctgctgggcg cacctgccca cgcggtatcc atacccggcg ttacaaccac aacgacaacg
     3781 gactcaacga ctgaaccggc cccggaaccg gatatcgaac aaaaaaaagc ggcctatggc
     3841 gcactggcgg atgtgctgga taatgacacc tcgcgtaaag agttgatcga ccagttgcgc
     3901 accgtcgccg cgacgccacc tgctgaacca gtcccgaaga tcgtgccgcc gacgctggtt
     3961 gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc gccattatgg tgaagccctt
     4021 tccgcccgct tcgggcaact ttatcgcaat atcaccggct ccccgcataa gccgtttaat
     4081 ccacaaacct tcagcaatgc gctgacccat ttttcaatgt tagcggtatt agtgtttggt
     4141 ttttactggc tgattcgcct gtgcgcactg ccgctgtatc gcaaaatggg tcagtgggcg
     4201 aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc cggcgatgat tatcggggcg
     4261 tttattatcg acctgctgtt actggccctg acattatttg tcggccaggt attaagcgac
     4321 aacctgaatg caggcagtcg caccatcgct ttccaacaaa gtttgtttct caatgccttt
     4381 gccctgattg aatttttcaa agccgtacta cgcctgattt tttgcccaaa cgtggcggag
     4441 ctgcgcccgt tcacgattca ggacgagagc gcccgttact ggagtcgtcg cctgagctgg
     4501 ttaagcagtt tgataggtta tggcctgatt gtggccgtgc cgattatctc taatcaggtg
     4561 aatgtacaga taggtgcgct ggcgaacgtc atcattatgc tgtgcatgac cgtctgggcg
     4621 ttgtacctga tctttcgtaa taaaaaagag attacccagc atttgctcaa ctttgcggag
     4681 cattcgctgg cctttttcag cctgtttatc cgcgcctttg cgctggtgtg gcactggctg
     4741 gcaagcgcct attttatcgt gctgtttttc ttttcgttgt tcgatccggg caacagcctg
     4801 aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta ttggtatcgc agcgtttgtt
     4861 tccggtatgt tttcccgctg gctggcaaaa accatcactc tctcgccaca tactcagcgt
     4921 aactatccag agttgcaaaa acggctgaat ggctggcttt cggcggcgct gaaaacggcg
     4981 cgtattctga ccgtctgcgt ggcggtaatg ttgctgttga gcgcatgggg attgttcgat
     5041 ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg tagatatcct gatccgtatc
     5101 gcacttattc tttttttctc ggcggttggc tggacggtgc tcgccagttt gatcgaaaac
     5161 cggctggctt cggatattca tggccgcccg ctacccagcg cccgtacgcg taccctgctg
     5221 acgctgttcc gtaacgcgct ggcggtgatt atcagtacca tcaccatcat gattgtgttg
     5281 tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg ccggggcatt aggtctggca
     5341 atctcgtttg gttcgcaaac gctggtgaaa gatattatca ccggggtatt tattcagttt
     5401 gaaaacggca tgaacactgg agatttggtg actatcgggc cgttgaccgg cacagtggaa
     5461 cggatgtcga ttcgctccgt gggcgtgcgc caggataccg gggcgtatca catcattccg
     5521 tggtcttcga taaccacctt tgctaacttc gtccgcggta ttggttcggt agtggcaaac
     5581 tacgatgttg atcgccatga agatgctgat aaagccaatc aggcactgaa agatgcggta
     5641 gcggaattga tggaaaacga agaaattcgc gggctgatta ttggtgaacc gaactttgcc
     5701 gggattgtcg gcttaagcaa taccgcgttt acactgcgtg tttcgttcac cacgctgcca
     5761 ctcaaacagt ggacggtccg ctttgccctc gacagccagg tgaaaaaaca tttcgacctg
     5821 gcaggtgttc gcgcgccggt gcagacttat caggtgttgc ctgctccggg cgcggccccg
     5881 gctgaaccgc tgccgccggg ggagccaacg ctttaacgct ggcgattgac aaaacggcgg
     5941 cgctgctcgt cgttcataaa ggtccaggca ataaagcgac tctgcttttg cccctgggcc
     6001 atctcttttt ttaccacctt caccgcaccc acgtccgtca gggcacgata caacggcggt
     6061 aagttttcac cacgagaaac cagcgaggta aaccacatca cctgcttcgc gaagcctttg
     6121 ctctcttcaa tcatcttttt gataaaggcg acttcaccgc cttcacacca caactcctgt
     6181 tgctgaccac caaagttcag tgcatcgtct ttgttcagcc ccaggttacg gcgtttacgc
     6241 tcactacctg cccgcgccgc agcggcggaa tcgtggaatg gcgggttaca caaggtcgcg
     6301 tcgtattgct cgtttttatg gatgatgccg ttaaaaatcg ccccactctc tttttgccga
     6361 cgcagacgaa tggcgcggtt aagccccgga ttagcactga tgatcgcctg cgcactgctt
     6421 aacgcctggc tgctggtttc actaccggta aaacgccagc catattcatg tacgccaatc
     6481 agcggataaa tacagttcgc accaacgccg atatcaagaa tgctggcatt cgctggaatt
     6541 gtaccgcttg cttctgccag taaatcggca aggtgatgaa tataatccgc ccggcccggt
     6601 actggtgggc agagaaaacc gtcggggata tcccagttcg ctacggcgta aaaatgggcc
     6661 aacaacgcct tattgagcgc cttcaccgcc agcggattgg caaagtctac gctttgctcc
     6721 ccggcgggtg taagcgtgag gaattgcctg agttcaggat tgacctgaca aagcgtggcg
     6781 agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc ccggtttctg ggcggacata
     6841 gcattctcct ttaagcagcg gcgtaagata cccgcataac gcgacagggt aaatatctgg
     6901 ctgcgtttat cattccccaa aaccttaaca tggctatggt ttatggcaat tcatcataaa
     6961 aatcagcatt atgcatgtca ttgatttgtc ttattttgta aaaatatatc gttcaaaaaa
     7021 taagcgtatc tctgtcaatt ttttgcgtca ttttcatcgc ccaggcgacc tacacttatc
     7081 aggcactacc cgttgtattt atgaggatgt cattatgaaa aagtgcctca cactactgat
     7141 tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc gcgcaaccga tgagtaacct
     7201 ggacagcagt cagctacggc ccgccggtac cgtttcggca actggcgcat caaacctaag
     7261 cgatctggag gataaattgg cagaaaaagc gcgcgaacaa ggcgcgaaag gttatgtcat
     7321 taattccgca ggcggaaatg accagatgtt cggtactgca accatctaca aataacccca
     7381 ccaacgtaac tgcattcgcc tctggatgca gttttcgcct gttttgtaat caaaaaacat
     7441 caaatatgaa cgcaatgtaa ataaatgtat ttctttttcg cgcaatgggt gatagaaaat
     7501 cgcgctaagt gataatgctt atcaaaatta ttatcacttg cacgaacact atcacgagat
     7561 taacagtggc atcgcatccg cagagaggct ttctcgtggc agtgaaaatt tcaacatata
     7621 agaaaaagtc acctgcaaaa tggaaaacaa tcgcaatttc cctgccagac aatttcattc
     7681 gctcacgttc tttgccggtc tttgtattgg catcacgcct gtggctcagg cactcgccgc
     7741 cgaagggcaa actaacgcgg atgacacgct ggttgtcgaa gcatcaacgc cttcgcttta
     7801 tgcgccacaa caatctgccg atccgaaatt ctcgcgtccg gtagcggata ctacccgcac
     7861 gatgacggta atttctgaac aagtgattaa agatcagggc gcaaccaacc ttaccgacgc
     7921 gctcaaaaac gtccccggcg tgggtgcgtt ttttgcgggt gagaacggta actccaccac
     7981 tggcgacgcc atttatatgc gtggtgccga tacctctaac agtatttata ttgatggcat
     8041 tcgcgatatc ggcagcgtct cgcgcgacac cttcaatacc gagc
//
LOCUS       DAOFQD010000176         8026 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.176, whole genome shotgun sequence.
ACCESSION   DAOFQD010000176 DAOFQD010000000
VERSION     DAOFQD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.176"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            47..1825
                     /locus_tag="QBF46_004346"
     CDS             47..1825
                     /locus_tag="QBF46_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDQ6676453.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1865..2344)
                     /gene="xdhC"
                     /locus_tag="QBF46_004347"
     CDS             complement(1865..2344)
                     /gene="xdhC"
                     /locus_tag="QBF46_004347"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDQ6676454.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2341..3219)
                     /gene="xdhB"
                     /locus_tag="QBF46_004348"
     CDS             complement(2341..3219)
                     /gene="xdhB"
                     /locus_tag="QBF46_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDQ6676455.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHTEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3230..5527)
                     /gene="xdhA"
                     /locus_tag="QBF46_004349"
     CDS             complement(3230..5527)
                     /gene="xdhA"
                     /locus_tag="QBF46_004349"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDQ6676456.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPIS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5942..6697
                     /locus_tag="QBF46_004350"
     CDS             5942..6697
                     /locus_tag="QBF46_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDQ6676457.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSITRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6776..6849
                     /locus_tag="QBF46_004351"
     tRNA            6776..6849
                     /locus_tag="QBF46_004351"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6808..6810,aa:Gly,seq:ccc)
     gene            7083..>8026
                     /locus_tag="QBF46_004352"
     CDS             7083..>8026
                     /locus_tag="QBF46_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676458.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAGNDSNLLV"
BASE COUNT         2061 a         1888 c         2022 g         2055 t
ORIGIN      
        1 aacgtcttca atgatcgact tttaagtaag tctggaaggt aaagctatgg agcttgctac
       61 tacgcagtca gtattgatgc aaattcaacc gacaattcag cgttttgcca gaatgcttgc
      121 cagcgttttg cagcttgagg ttgagatcgt tgatgaaaac ttgtgtcgcg tcgccggaac
      181 gggcgcgtat gggaagtttc ttggtcgcca gttgagcggc aactcacgcc tgctccgcca
      241 cgtcctggaa acgaaaactg aaaaagttgt gacacagtct cgcttcgatc ccctttgcga
      301 aggttgcgat agtaaagaaa attgccgcga aaaagcattt ctgggtacgc ctgtcatttt
      361 acaggatcgt tgtgttgggg tgataagttt gattgccgtt acccacgagc aacaagagca
      421 tatcagtgat aatttacgcg aattttctga ttatgttcgc catatatcca ccatttttgt
      481 ttcgaaactt ctggaggatc aggggccagg agataacatc agtaaaatat tcgcgaccat
      541 gatcgataat atggatcagg gcgtattagt tgttgatgat gaaagtcggg ttcagtttgt
      601 taatcagact gccttaaaaa cacttggtgt tgtacaaaat aatattattg ggaaacctat
      661 ccgtttcaga ccattaacat ttgagagtaa ttttactcat ggacatatgc agcatattgt
      721 ttcgtgggac gataaaagtg aattaatcat tggtcaattg cataacattc agggccgaca
      781 attattttta atggcatttc accaatcgca taccagtttt tctgtagcaa atgcacctga
      841 tgaaccacat attgaacaat tggttggcga gtgccgtgtt atgcggcaat taaaacgact
      901 cattagccgt attgcaccca gcccatccag cgttatggtg gttggtgaaa gcggcacggg
      961 taaagaagtc gtcgcccgag caatccataa gttgagcgga agacggaata aaccctttat
     1021 tgctatcaac tgtgccgcga ttccggagca gcttctggaa agcgaactgt tcggttatgt
     1081 taaaggcgca tttactggcg cttctgccaa cggtaaaaca gggttgattc aggcggcgaa
     1141 tacgggcacg ctgtttctcg atgaaatagg tgatatgcca ttaatgttgc aggctaaatt
     1201 actgcgcgct attgaggcgc gtgaaattct gccgattggt gccagtagcc caatacaagt
     1261 cgacattcgc atcatttctg caactaatca gaatttggcc cagttcattg ccgaaggtaa
     1321 attccgcgaa gatctcttct accgacttaa tgttatcccg ataactctgc caccgctgcg
     1381 tgaacgtcag gaagatattg aactattggt gcattacttt ttacatctgc atacccgtcg
     1441 tctgggatcg gtttatcctg gcattgctcc cgatgtcgtc gaaatattgc gtaagcatcg
     1501 ttggcccgga aacctgcgcg agttaagcaa tttgatggaa tatctggtta acgtggttcc
     1561 ttcaggtgaa gttatcgaca gcacgctatt gccgccaaat ctgctgaata atggcacaac
     1621 ggagcaaagt gatgtaacag aggtcagtga ggcgcacctg tcactcgatg atgcgggcgg
     1681 cacggcgctg gaggagatgg aaaagcaaat gatccgcgag gcgctttcac gtcataacag
     1741 caagaagcaa gttgctgatg aactgggcat cggcattgct acgctctatc gcaagattaa
     1801 gaaatatgag ttgttaaaca cataacctca gtgaatccgg cctgaattca ggccggatat
     1861 ccttttactt cgttttctcg caatccagaa ctgtatttac aatcatctga taccccgtgc
     1921 agcgacaaag atttcccgcc agtccgcgac gaatttccgt aatggttaat ggcttctcgc
     1981 gtggtttcgc cagcattgcc gtggtagcca taatcaggcc aggcgtacaa aacccgcact
     2041 gcactgcgcc ggatttcgca taagcctgct gaacatgaga aagttttccg cctttcgctt
     2101 caccttccag cgtgcggatc tcttttcctt cagcccaggc ggcaaggtat aagcaactgt
     2161 ctattgctgt gccgtcgacc aacaccgtac aggcaccaca ttcacccacg cagcaccctt
     2221 gtttgacact tagcagtcct tgttcgcgga gtaattccga gagcggcgtg cctggtgcgg
     2281 cgtgaagctg aaaaggcatc ccgttaatgg tgcattcgat ggtaattgtt tcgctgtgat
     2341 tcattgcaat tttccccccg ccgcggcgac ggcttcgcta atcacttttt tggtcatcgt
     2401 ctggatgaga tgcagacgaa actctttact ggcccgccat gaagaacgcg gggcgacatc
     2461 ttgcaggaca gattcgctga tagcttccag cgtttgcagg tttaatggcg cattttgtgc
     2521 agtctgttcg gtatgttggc agcgaatcgg cgttggcgcg gcaacaccaa atgccaggcg
     2581 taattcgctg aaattgccgt tatccagtcg gcaatgtgcg gcgcagccaa tcgttgaaat
     2641 atccattgcg tcgcgcatgg catatttaaa atgcgcgctg cccgcgtgtt ctttcggctg
     2701 tggcggaaaa tgaaaggcga cgaggatttc gtcatgctca agagacactt tgcccggccc
     2761 ggtgtgaaag ccattaatcg ggacgaaacg aacaccgcgc ggggagtgga tctccagttt
     2821 cgcgtcataa attagcgttg gcgtggcaga atctgcgctg gtggcaccgt tgcaaatatt
     2881 tccaccgtag gtagcgacgt tacggatctg cggtccagca atggacgtgg ccgcagcaca
     2941 taacgccggg agatgacgtt gagttatagg atcttctatt agctgggtaa atgtcgttgc
     3001 agagccgata cgtagcgagc catcttccgc cagcgtaatt ccccgcagct ccgccagatt
     3061 atgaatatca acaatatggc gataacggtc attgtgatgg tggagctgaa tcagtacgtc
     3121 agtgccaccg gcgagcagtt tggcctgcgg gttgtcagcc agcaggttga tggcatcggc
     3181 aagggttgct gcgcgatggt aagaagcaaa atcaaacatg atgttatcct caaatcaatc
     3241 ctgccagatg gaactcttca tataaccgtt ttggcgtcag cggcagtgta ttgattgcaa
     3301 caccggtagc catcttcacc gcgttacgaa tagcagcggc aacaggaatt attggtggct
     3361 cacccagtga cttatgtccg tatgcggatt gcggctcatt gatttcgacg aacgcgcttt
     3421 ccagttgtgg cagatccggc atggtcggca ttttgtaatc cagcagattg gggttacgga
     3481 ccacgccgct tttagcatcg atgatcatct cttcaaatag cgcccagcca atgcccattc
     3541 ccattccgcc gtgtacctga ccttctgcca gcagtggatt aagaatatgc cctgaatcat
     3601 gaacgttgag gatgcggttg atggtgactt tgcacagcgc aatatcgacc gtcagatcaa
     3661 caaaggtaca gccaaacgcc ggtgggttag tggtggtttt gatggagctt tcagcagaga
     3721 gctgcccgcc gcgttcaggg tggtagaaag cgtccatcgc caaatctttt aacgacatta
     3781 acggctcttc cggtcgttca accagcacga tatggccttt tatcagggtc agattcatcg
     3841 ctgactgatg tagcatgact gcggcgtgag cgatgatttt ctcttttaat aatagtgccg
     3901 cactgcgcag cgcaggcgcg gcaacatagc tctggcgtga ggcaaatgcg ccgggatcga
     3961 acggcgtaac gtcggtatct tgagttgaaa taacgcgaac gtcgctgatc ggaaccccca
     4021 cggtttctgc caccatttgc gagaagacgg tgtcggcacc ctgaccgatt tccgtcgcgc
     4081 cgctttgcac gttgatggtt ccatcctgat tcatcagaag gcgcgcgcct gctatttcta
     4141 cgccgacagg ccaggtgtta gaggtgtagc taaaacaggc gacgccaacg ccgcggcgca
     4201 aattgccttg ctggttctgg cattctgcac ggcgtttttc ccattcaaag attttccggc
     4261 ctttttcaag acactccggc aaccctgcgc tgtaaatacg tttgcccgtg agcggattag
     4321 catctccttc gcgggcggcg ttgcgtaaac gaatttcaac aggatcaata cctaacgctg
     4381 tcgcggcgtc atcaagcata gactcaacgg caaatacgac ttgtggcgcg ccataaccac
     4441 gcatcgcacc agccgagggg aggttggtat agcaggtctt tgaactgtaa gcgtaggcac
     4501 aacgaggata aaggtaagcg actttattcc cccccgcaga agcgatggag tgcccgtgag
     4561 atgcataagc gccggtgtta gacagaacat ccagactata acctttcaat gttccgtcgc
     4621 ggttcacgcc catttgcccg tcaatggtaa aagcgtggcg ggtacgggtt gcgaggaaac
     4681 actcttcacg gctaagggaa actttcaccg gaatgccgcc aagcttgctg gtcaggaatg
     4741 ccgccattgg ctcttccagt acatcctgtt tattaccaaa accgccaccg acaaatggtt
     4801 tgatgactcg tacgcatgac cagggaatat ccagcgcctg accaaccacg cggcgaacaa
     4861 tgtgcgggat ctgggtgctg gaaacgatgg taattcgcga gtcatcctcc atccacgcca
     4921 gcgatgttac gctttccatg tgacaatgtt gaataacggg ggtctgatag tgcccctgta
     4981 cctggtagtc ggcggcatcg attgtttgtt ggacattacc cgtcgacatc gtgctttgtt
     5041 tcagtaaatt gccaccgtta tggattggtg cagcgtcttc tgccagcgcc gcttctggcg
     5101 tggtgataac gggtaattct tgccactcaa tgctgaccaa ttgcgccgct ttttctgccg
     5161 tgagttcatc gcgggccacg acgatggcaa cggcgtcacc atgatgacga acatggcgag
     5221 ttagcagtgc gcgatcggcg gtatcgcgct tgttttcgtc aagtgtccag gcatgcccag
     5281 ctgtagcgaa tggaatatca ggcacatctt cccaggtaaa aatcgccagt acgcccggca
     5341 aacttctggc ttgttcatca ttaatactta cggcataacc atgtgcgata gggctacgta
     5401 catatttcgc ataacacatg cccgccataa cataatcgtc agtatatcgt gcccgcccgg
     5461 tgaccttagc aatggcatcg acgcgcatgc atgattcacc cgtagcggtt gcttcccgcg
     5521 cttccataga aatcccctcg atttgaacat ttggttctta tggcaaagat ttagtctaag
     5581 caggcaagat ttacgccaga aacgacgata agcgttaggg gaggtaggaa gtgtgatgac
     5641 gttaaaaaaa cacagatatg atacgaaaca ggactctgaa taggggcagt gatacgtatc
     5701 aatatgagtg gcgataaaat caatgtgata aattgggatt attacgcatc aggatgggaa
     5761 atagggtaaa aagtcaggaa gttcgatacc tctacagcga atattttgct gatgatactt
     5821 ccctttgcca aatgaaatta acctctttct ctattaccac gtttttccag aagcaagaga
     5881 ttgggtatca agaggctggc tgctatgata aggcgtcttg ttttttaagc gaggaaagat
     5941 tttgagtgcg ggacgcctga ataaaaaatc tctgggtatc gtgatgttgt tatcggttgg
     6001 actgcttttg gcgggctgtt cgggtagcaa atcatccgat acaggaacgt attccggctc
     6061 cgtttacacc gtgaaacggg gggatacgct atatcgtatt tcgcgcacca cgggaaccag
     6121 cgtaaaagaa ctggcgcgac tgaacggcat ttccccccct tacaccattg aagttggtca
     6181 gaaactaaaa ctgggtgggg cgaaaagtag cagtattaca cgtaaatcaa ccgccaaatc
     6241 aacgaccaaa accgcatcgg ttacaccgtc atcagcggta ccgaaatcat cctggccgcc
     6301 agtagggcaa cgttgttggt tatggccaac gacagggaaa gttatcatgc cgtattcgac
     6361 agcagatggc ggcaataaag ggattgatat ctcagctcca cggggtacac ctatttacgc
     6421 cgcgggtgca ggaaaggtgg tgtatgtggg caaccagctg cgtggctacg gtaatctcat
     6481 catgattaaa cacagtgaag attacattac ggcttacgcc cataatgaca cgatgctggt
     6541 aaataatggg caaagcgtga aggctgggca aaaaatcgcc actatgggga gcacggatgc
     6601 ggcatctgtt cgcctgcatt tccagattcg ttaccgtgca acggcaattg atccgctacg
     6661 ttacttgccg ccgcagggca gcaagccaaa atgctgatgg cgaattaatc agcagtcagc
     6721 agcgcggcac ttgctaagga gagcgtaagg tttataatgc cttacgcatc tcgaagcggg
     6781 cgtagttcaa tggtagaacg agagcttccc aagctctata cgagggttcg attcccttcg
     6841 cccgctccaa tttatcttca cctcatcaat ctgccatttt tgctgtatct gattttctat
     6901 attatcttaa ttcaatcgct tcagactttt cgcaatcgcc gtaatatttc cccattttgt
     6961 tgtaaataaa aagcgtttac gcaatgcaat ccttttgtaa caacccaggt aaaacacctt
     7021 attttcaagt tgttaaataa gtggttatct acgcttttaa caaacgattg atagaatatt
     7081 ttatgaaagg gaaatctgca ttaactcttc tgctcgctgg tatattttcg tgcggcacat
     7141 gccaggcgac aggggcagaa gtgacgagtg aatctgtatt taacattctt aattccacgg
     7201 gggcagcaac agataaaagt tatttatcgt taaatccaga taagtaccct aattatcgtc
     7261 ttctgattca ctcagccaaa ttaaaaaatg aaataaagag ccactatacc aaagacgaaa
     7321 ttcaagggtt gttaacatta acagaaaaca cacgaaagtt aacgttgacg gagaaaccct
     7381 ggggaacatt tattcttgct tctactttcg aagatgacaa aacagcggca gaaacccact
     7441 atgatgctgt ctggctgagg gatagtttat ggggctatat ggcattagta tcagatcagg
     7501 gaaatagtgt agccgcaaaa aaggttctgc tcaccctgtg ggattatatg tctacgcccg
     7561 accagattaa gcggatgcag gacgtcatta gcaaccccaa acgactggat ggagttcccg
     7621 ggcaaatgaa tgctgtgcat atccgatttg acagcaactc ccccgtgatg gcagatgtgc
     7681 aggaagaagg caagccgcag ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc
     7741 ttgatctcct tatacaagca atcgacactg gtacaattaa cgctgaagat tggcaaaaag
     7801 gtgaccgatt aaaatcggtt gcgctgctga ttgcttatct ggataaagcc aatttctatg
     7861 ttatggaaga ttccggagca tgggaagaag atgcccgatt aaacacatct tccgttgcac
     7921 tggtgacctc tggactggaa cgtttgtcga acttactctc gaagaaagac tctgtttttg
     7981 tctctgattt actacgcgaa gctggtaatg actccaactt attggt
//
LOCUS       DAOFQD010000177         7983 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.177, whole genome shotgun sequence.
ACCESSION   DAOFQD010000177 DAOFQD010000000
VERSION     DAOFQD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.177"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..206)
                     /locus_tag="QBF46_004353"
     CDS             complement(<1..206)
                     /locus_tag="QBF46_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDQ6676459.1"
                     /translation="MSLTDIKAKNAKPLEKEYKLTDGFGMFLRVTPKGSKYWQMAYRF
                     EGKQKLFSIGVYPAVSLSDARQRR"
     gene            complement(387..462)
                     /locus_tag="QBF46_004354"
     tRNA            complement(387..462)
                     /locus_tag="QBF46_004354"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(427..429),aa:Asn,seq:gtt)
     gene            complement(563..1360)
                     /gene="mtfA"
                     /locus_tag="QBF46_004355"
     CDS             complement(563..1360)
                     /gene="mtfA"
                     /locus_tag="QBF46_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDQ6676460.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            1454..1543
                     /locus_tag="QBF46_004356"
     tRNA            1454..1543
                     /locus_tag="QBF46_004356"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1488..1490,aa:Ser,seq:cga)
     gene            complement(2113..2643)
                     /locus_tag="QBF46_004357"
     CDS             complement(2113..2643)
                     /locus_tag="QBF46_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDQ6676461.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(2986..3636)
                     /gene="zinT"
                     /locus_tag="QBF46_004358"
     CDS             complement(2986..3636)
                     /gene="zinT"
                     /locus_tag="QBF46_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDQ6676462.1"
                     /translation="MAIRLHKLAVALGVFMVSAPAFSHGHHSHGKSLTEVEQKAANGV
                     FDDANVQNRKLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(3893..4528)
                     /gene="msrQ"
                     /locus_tag="QBF46_004359"
     CDS             complement(3893..4528)
                     /gene="msrQ"
                     /locus_tag="QBF46_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDQ6676463.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLALLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(4529..5533)
                     /gene="msrP"
                     /locus_tag="QBF46_004360"
     CDS             complement(4529..5533)
                     /gene="msrP"
                     /locus_tag="QBF46_004360"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDQ6676464.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(5642..6055)
                     /gene="hiuH"
                     /locus_tag="QBF46_004361"
     CDS             complement(5642..6055)
                     /gene="hiuH"
                     /locus_tag="QBF46_004361"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDQ6676465.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQIGKPAAD
                     VTVTLEKKSDNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            6188..6859
                     /gene="hprR"
                     /locus_tag="QBF46_004362"
     CDS             6188..6859
                     /gene="hprR"
                     /locus_tag="QBF46_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDQ6676466.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            6859..>7983
                     /gene="hprS"
                     /locus_tag="QBF46_004363"
     CDS             6859..>7983
                     /gene="hprS"
                     /locus_tag="QBF46_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDQ6676467.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSF"
BASE COUNT         2277 a         1663 c         1800 g         2243 t
ORIGIN      
        1 tcacggcgtt gtcttgcgtc agaaagagaa actgcagggt aaacaccaat agagaagagt
       61 ttttgtttcc cttcgaagcg gtaagccatt tgccagtatt tcgaaccttt aggggtaaca
      121 cgaaggaaca taccaaagcc atcagtaagc ttgtattcct tctcaagggg ttttgcattt
      181 tttgctttaa tatcagtcag tgacatgaaa acaccccctc atttgttggt aaaagggaaa
      241 tcgaaccagc attaccagca tttttaccat caaaatgata tggcttcgag tggtttttaa
      301 tgaacgaggg tgaacgactg agaggggata accaattgat agaaatgcag aaagcagacg
      361 ttagtgaacg tctgctttct tgaaaatggc tcctctgact ggactcgaac cagtgacata
      421 cagattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgtg aacggggcgc
      481 atattactta gcggtacctt gtctgtcaac actaaatttc ataagtcatt tcaattggtt
      541 aattaatctg caaagttgtt aattaatgaa cattcgtcgc cgaaaacgag tctgtatcat
      601 tagtgcgatg cagtctctgc aaaggatctt gttgataaaa ttggcagaaa cgttgccaca
      661 atgaagggaa acgaggagca aaaagttctg gggcgctaaa gaaatattca gaaagtacgg
      721 caaaacattc agcaggatca ctggcagcat aagcatcaat gctcgccgca ttctcgccaa
      781 ccaattcgat ttcttcctga atgttgttca ttgcagcatg aagatcgtgt tcccagccag
      841 caacctcacg caacggaata aagggaactc cgctggcgcg atcgccgtta cgggtgtcca
      901 gcttatgagc gacttcatga ataatcaggt taaaaccaga tgcatcaaaa gaatcttgta
      961 tatccaacca gttcaaaacg ataggccctt gctgccagct ctgacctgac tgaacaatac
     1021 gttggttatg caccagaccg atatcgtctt cccattcatc atcgaccaca aatggcgcag
     1081 gataaattaa gacttcatga aaaccatcca gccattccag tcctaactcc agaacgggta
     1141 ggcaaaatag aagtgctatc cggcagcttc ttaatgaatc cagttcaaag ccctgtaaag
     1201 gaacaagccg cttttgctgt aaaaaacgtt cggcaagagt gactaatttg ctttgttcct
     1261 gttccgtcag acccgttaaa agggggatcg atagtgcttc ctgccaggga agggcagttt
     1321 gatgtgctga ttcttgtact ttccagggcc acttaatcat cgttttgctc gcaaactcgt
     1381 cacttgaaca aaattgcacg gacagggact gttaaaatgc caaatttcct ggcatcatgg
     1441 caaccatctg aacggagaga tgccggagcg gctgaacgga ccggtctcga aaaccggagt
     1501 aggggcaact ctaccggggg ttcaaatccc cctctctccg ccactttatc aatgacttat
     1561 ctcctgactt cccgccttgc ttttcctaaa cagaacaatc gtagaatatt cttgaagggt
     1621 tagatcgtca ctgttttctg ttcgatactg tgacattcag cacttgatcc gctatggatc
     1681 tgacaggaag ctttcgagcg aaaatgtgca gttattcagt cgttttctta tcgctcacca
     1741 ttattctttt agatattcat cctatgaaac tgccgcaaag ttggtggtgg gaactgaagt
     1801 tgcgtagaga aggggtcaat aattgagtca aaaaaggaac gcggccgtca gattaaagcc
     1861 gcgggacaaa gtccataatt tccaagagtt gttgaatttc ccagcttttt tgaaagcctt
     1921 tgtatcatac taacaagtaa aaaaggttta aataccaaaa ttaacctgta tttttgataa
     1981 gtcttaccat tgtacgattt acacagtttc tgctcctcct tgttgatagt ggaaagggtg
     2041 cattgtgcac ttgcgtaaac atttatgacg cgttgatgtc catggttcac atttccttgt
     2101 caggggatcg acttatttgc ggcgaggcat cattcgtaac agagtattat ctttctgaat
     2161 gtagtgatga aagagtgctg cgccagcgtg agctgcgatt aaaaagtaac ctatattcgc
     2221 ccaggtttcg tgaatatttt ttatcagtgc tttaatttcg ctgtttgggg taacaaaggg
     2281 agacacattg aaaccaagga aactccacga ttttccactg taagccatta aagcaatccc
     2341 cagcagagga agtgcaagga aggttatata aagcatgatg tgcattagcg aagccgcttt
     2401 catttgccag gcaggtggcg gtggtacaat agaaggatca ctataacggt gttttataat
     2461 caggcgtgaa aacattaaca cccaaacgaa tatacccgca ttgtaatgtg tttctcgcat
     2521 cagcagataa gtactactac ctttaggaaa ccagccacgg agttccatcg cggcataggt
     2581 tattgcaacg aaaagcagcg ttatccaatg taaataaatt tgagtttttg agaatcggtt
     2641 catttttgta gctcctgtaa tttttacagt caagctatcc cacgaacctt aaaaattcct
     2701 tatacagatg tggttttaag aatttgttat ttaaaaaatt gatagtgcac attgtaacca
     2761 gccaatataa gcaatggcgt gagcacaggt atctttctct gttggccgta ttgtgtatgg
     2821 aatccgttat tggttaactc aatgcgggtt agcttattaa tatacagaca cgtcatagtg
     2881 atgagcgtgt ctgtagctag cttgcgttca gtggcgtttg tggatggacc agtggcagtt
     2941 atgatgactt actaaagcgg catcgaggcg ttatcatgag aatactcaat gagacatcat
     3001 ttcctcgacc acttcttcgc tactcaactg atatggataa tacgttggcc agttttccat
     3061 ttcatttaat aaagattgct gtgaatcatt acccataaaa atgtgaaagt gagatgattt
     3121 tcgtggtgca attatatgat cgctaaattg tatatattta ggggctttgc tttcaggatc
     3181 tttacattcg aataagtaac gaacgccttt cttgcctgat ttataggtga gtattttgta
     3241 tccatcgtaa tcatatttac aggatgttgt ttcattattt ctatggaatt caacaatgcc
     3301 gtcctcaatg ccaatcatct cgatatctgt tgcataacct ttgtgataat aatctttaat
     3361 ttcagcaaat gttttagttt tatctgcatc cgctttcttc tgaaagacgg ggtcaagttt
     3421 gccactttgc agtaaaggat aaacggattg ccagactcca tcccagtcac tcagctttcg
     3481 gttttgtaca ttggcatcat caaaaacacc attagcagct ttttgttcga cctctgttaa
     3541 ggatttgccg tgtgagtgat gaccatgcga aaaggcagga gcgctaacca taaagacacc
     3601 taaagcaaca gccagtttgt gaagacgaat cgccaaaaca gtttcctcca gagtcattgt
     3661 atatgatgtg taatgttata ttgtaacata taacaaaatg caacaactct taggagacaa
     3721 gagaatagtt ctctcaggta tgattatcta tcttattgaa ataacataaa ttatgttggt
     3781 aatttgaggt tgatgacaaa cgaaacataa cctgatgtga tctgcgtagg ccggataggg
     3841 cgctcgcgcc gcatccggca gggataacgc gtactttgtc ggcaatatga agttatacag
     3901 ataatttgtt gtgaacctgc ttgcgtaacc ggttaaaaag ggaacgcaac ttcttatacc
     3961 gtaaggctaa aagcagtaaa gccagcccag cgtagatgag cggctgcggt gaaataatct
     4021 tcactgacca cagataatgt attggggcca ggatcgcgac aagatagacg aaattatgca
     4081 actgttgcca gtgcttgccg agttttcgct gcatcgcctg ggttgaagta aacgctaaag
     4141 caagcaaaat aacccagctg ataattccta acgttaaata aggccgggta attaactcct
     4201 tgcctaataa cgccagatta ttcacgccca gctccagcaa tgcgtaactg gttaaatgca
     4261 gtgtcgccca ggcaaagcac cataagccta acaggcggcg agtgcgtatc aataacggct
     4321 gttttgcgta gcgtgccaga ggtgtgatta acaaggtcgc cagcaaaaat ttcagtgcag
     4381 tgcgaccggt aaaatgctga atatctttca ccggatcggc acccagtcca ccgtgattga
     4441 tcgcccagac cagccagaga aacggcaaca atccggcaag atgcaggcta actttcagcc
     4501 atgtcacctg ttttgctgtc agacgcattc agaaattctc ccgcaaatcc aggccacgat
     4561 acagcgatgc cacctggtcg gcgtaaccat taaacagtag cgttggctgg cgctgtacat
     4621 cgaggatgcc gcctgaacca ataaatcgtt cggtagcctg tgaccagcgc gggtgatcaa
     4681 catgcggatt aacgttggcg taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg
     4741 ttggcggacg ctcgcgggtc agcttaatac tgacgatcga tttaatccct ttaaagccat
     4801 atttccacgg cacaatcagt cgcaccggcg cgccattttg tggcggtaac gccttgccat
     4861 aaacacccac ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc aatccttcga
     4921 cataaggata tttcagcccg ccgccgataa agcggtcctg ctggcctggc atctgttccg
     4981 gtgcataaat tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg gcaagcgcca
     5041 gcaatttgtg cagcggaaaa ccaatccacg gcaccaccat cgaccatgct tctacgcagc
     5101 gcatacgata aatacgctct tccagcggaa agcgacgggt taaatcatcg tgatcgaggg
     5161 tcaatggttt tgccacttcg ccgctgattt tcagtgtcca tggatcggtt ttcaggctac
     5221 cagcattagc ggcgggatcg gctttatcca gcccgaattc atagaagttg ttataaccgg
     5281 agactttatc tactggcgtc agtggcaggt tattttgcca ggcggcaggc ttgctgaact
     5341 ccagcggttt tccggcgggg gcgggcgggc gatcgttccc tttaaaccag ctaagcagat
     5401 cggcatgcgc agcgtgaggc aaagaaagtg cagctgcgct gatgcccagt gcttttaaca
     5461 cctgccgacg cttcataaag aataccgact cggccgtaac atctgattct tttaaaaatt
     5521 gattcttttt cataagccat cacacttttt tgtcgttagc cagcaagagc cgtttactgt
     5581 taatctgtca gggagtgtcc tccctaacag tagcagagat ctgcttttac atatttatgc
     5641 tttaactgcc acgataggtt gaatacccat attggctaag taataaaggc acatgataat
     5701 gctcgttcac tttattaata tgaaattcaa cggggatctc agggaagaaa ctttcaagat
     5761 tttgtttctt gaaatagtcc ccggttttaa atacgacacg gtaatcgcca gtagtcgcag
     5821 tttgctcggg ccacagtgcc ttaattcgtc catccttatc tgttttggcg gtattaagtt
     5881 gtaaccagcc gttgtccgat ttcttttcaa gagtgactgt cacgtcggca gcgggttttc
     5941 ctatttgctg gttcaaaatg tgcacgctaa gaatgttttg ttgtgcggca taaaccaaag
     6001 aaggtaatga aaatgctgcc gttgctaccg agagtactaa ataacgcttt aacatgttta
     6061 tatccttgtc atgtgaatga gttttgagga tagcgaaaaa atgtccaggg taaccttaca
     6121 gcagcattac aattttgtaa tgaaccaggc tgtttctata acatatgatt tatggcatat
     6181 tattttcatg aagattttac ttattgaaga taatcaaagg acccaggaat gggtaacaca
     6241 agggctttcc gaagcgggtt atgtcattga tgccgtttct gatggcagag atgggcttta
     6301 tcttgcgctg aaggatgatt atgcattgat cattctggat attatgcttc cgggtatgga
     6361 tggctggcag atcttacaaa cgttaagaac ggcaaagcaa acccctgtta tttgccttac
     6421 tgcaagggat tctgtcgatg acagagtcag aggactggac agtggagcaa atgattatct
     6481 ggtaaaacct ttttcatttt ctgagttgct ggcaagggtt cgggcacaat taaggcaaca
     6541 tcacgctttg aattcaacat tagaaatcag cggcttaaaa atggactctg ttagtcaaag
     6601 tgtgagcagg gacaatatca gtattacact gacgcgcaag gagtttcagt tactttggct
     6661 actggcctcc agagctggcg aaattatacc cagaacggtt attgcgagtg aaatttgggg
     6721 aatcaacttt gatagtgata ccaatacggt ggatgtcgcc attcgcaggc tccgcgcaaa
     6781 agttgatgat ccttttcctg aaaagctaat tgccacaatc cgtgggatgg gatattcgtt
     6841 cgtagcggta aaaaaataat gaaaagattg tctataaccg tccgtttaac cttgcttttt
     6901 atattgctac tgtctgttgc tggtgctgga attgtctgga ctctctataa tggcctggca
     6961 agtgagttga aatggcgcga tgatacaaca ctcattaacc ggacagcaca gatcaagcag
     7021 ttgttaattg atggggtaaa tccagatacg ttacctgtgt actttaaccg gatgatggat
     7081 gttagtcagg atatcttgat cattcatggt gatggcatca ataaaattgt taaccggaca
     7141 aatgtcagtg atgacatgtt aaataacata cctgctagtg agacaatcag cgcagctggc
     7201 atttacagaa gcattattaa tgacactgag attgatgctt tacgaattaa tattgatgaa
     7261 gtttcgccat cattaacggt tactgtggct aaattggctt cagccagaca taacatgctt
     7321 gaacagtata aaattaatag cattataatt tgcattgtcg ccattgtact ttgctcagta
     7381 ttaagtccgc tgttaatcag aacgggatta cgagagatca aaaagttgag tggtgtaacg
     7441 gaagcgctga attataacga tagccgagag cctgttgagg ttagcgcatt accgagagaa
     7501 ttaaaacctc ttgggcaggc gttgaataaa atgcatcaag ccttagtcaa agattttgaa
     7561 cgcctaagtc aatttgctga cgatctcgct catgaactta gaacgcccat taatgcatta
     7621 ctgggtcaga atcaggttac gctcagtcaa accagaagta tcgctgaata tcaaaaaaca
     7681 attgccggta acattgaaga gttggaaaat atttcgcggt taacagagaa catactgttt
     7741 cttgcccggg cagataaaaa caatgttttg gtgaaactgg actcgctttc tctcaataag
     7801 gaagtcgaaa atttgttgga ttatcttgaa tatctttcag acgagaaaga gatttgcttt
     7861 aaggtcgagt gcaatcagca aatctttgcg gataaaattt tactgcaacg aatgttatcg
     7921 aatcttattg ttaatgccat tagatattca ccagaaaaat cgcgtattca tataaccagt
     7981 ttt
//
LOCUS       DAOFQD010000178         7868 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.178, whole genome shotgun sequence.
ACCESSION   DAOFQD010000178 DAOFQD010000000
VERSION     DAOFQD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.178"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(277..762)
                     /gene="sixA"
                     /locus_tag="QBF46_004364"
     CDS             complement(277..762)
                     /gene="sixA"
                     /locus_tag="QBF46_004364"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDQ6676468.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(965..3109)
                     /gene="fadJ"
                     /locus_tag="QBF46_004365"
     CDS             complement(965..3109)
                     /gene="fadJ"
                     /locus_tag="QBF46_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDQ6676469.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDDEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVKHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(3109..4419)
                     /gene="fadI"
                     /locus_tag="QBF46_004366"
     CDS             complement(3109..4419)
                     /gene="fadI"
                     /locus_tag="QBF46_004366"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDQ6676470.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDFNKARIMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4600..4884)
                     /locus_tag="QBF46_004367"
     CDS             complement(4600..4884)
                     /locus_tag="QBF46_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDQ6676471.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5256..6596
                     /gene="fadL"
                     /locus_tag="QBF46_004368"
     CDS             5256..6596
                     /gene="fadL"
                     /locus_tag="QBF46_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDQ6676472.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6961..>7868
                     /locus_tag="QBF46_004369"
     CDS             6961..>7868
                     /locus_tag="QBF46_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676473.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEVNGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIDSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVINELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISNLFA"
BASE COUNT         2098 a         2007 c         1793 g         1970 t
ORIGIN      
        1 agtttcaatt acatcctgcc tgattattca actctatttt ctgtattatc tgtattaata
       61 aatgataatg agtgtcaaac agacattatt catttcaaca tgttactttg ggcaaaatca
      121 agatacgcgc agaaaaataa tttccgaatg aaattagaaa gtgttttatt tatatatggg
      181 gagagtcttc gatagtagtg aggttgacag atatccgctg cctgatgagc aattatgccc
      241 aatgaatcta cctcattggg cataatttgg aactcatcag atagcttttg ccatcttcag
      301 attacacggg ctcatctgcc agttgaatgt gccgtttccg ctttcatcaa gcgttacgct
      361 ggcaatggcc gaggtggtaa acataggcgg cgtttcgccc ggacataact cggcaaccag
      421 atacccgact aacggtaagt gagagatcac cagtaccgaa gcaacacctt cattggtcaa
      481 cgcctgcaaa taggcactga ccagaccaac atcgccgcag ggcgttaact ccggcagaac
      541 ttctgcactg gaaggcaggt tcagacaatc ccctacttct tccagtgttt gctcggctcg
      601 caggaacggg ctcaccagaa cgcgttcgat ttccactttt tgacctttta gccagttcgc
      661 catcaggcga gattcgtcac aaccattagt ggtcagagga cgaacggaat cactggcggc
      721 atcgagggct gcgtcgccgt gacgcatgat aaaaacttgc atattgcacc gcttttgtta
      781 accagattcg cccgttttct ttcatctgaa accagagaaa gaaaacggtg gccggcattg
      841 tgccttatcc attcaccgaa tgaaacgctg ttttttacct caatggcgta agtatagtca
      901 atatgcgttt acattttgag caacactccg ccattcagcg cggattcata tagctttgac
      961 cttcttattg caggtcagtt gcagttgttt tccaaaaact ttccccacgc ttgctcatct
     1021 cgactaaacg ctcgcaaggg gtaaaacggg aaccatactg cgtggccagt cgttgcatta
     1081 ttgcaaccac ttcgcccgcg ccgagagaat cgatatagcg gaacggtcca ccgagaaatg
     1141 gcggaaaacc aatgccaaat accgcaccaa tatccccgtc acgcacgcta cggataacct
     1201 gctcatcaac acaacgtact gcttcattca gcatcaacat cacacaccgt tcagcaacct
     1261 gcggtgcgga gagtcgcccc tgcccttgtg cgccaatgag cgggtaaatg gcaggatcga
     1321 cctgtttttt gcttttacgc cctttctgac cataaagata gaaaccccga ccattttttc
     1381 tgcctttgcg atcgtcgttc aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt
     1441 ctccataagc ggcttccagt acaggaataa ttttagtccc ggtgtcgatt cctacctcat
     1501 ccaaaagttg gattggacct accggaaaac caaatttcac tagcgcggca tcaatgtgct
     1561 ttacccgttc accttcggtc agcatgcgga tagcttcatt aatgtaaggc gctaagatgc
     1621 gattgacgta aaacccggct ttgtcacgca cgacaatggg cgttttgccc tgttttttcg
     1681 ccagttttac cgtagtggcg atggtttgcg ctgatgtacc cgcatgagga ataatctcca
     1741 ccagcggcat tttttccacc ggactgaaga aatgcaggcc gataacttgc tcaggtcgcg
     1801 cggcgtgagc ggcgatatca ccaatcggta gagatgacgt attcgaagca aagatggtat
     1861 gagcggcgca attttgctca acttccgcca ccatctgttg tttcaattcg agattttcaa
     1921 acaccgcttc aataatcaga tcgcgatggg caaagccgcg atagtccgtc gttccggaga
     1981 ttaatgccag ctgtttgtca cgttcgctgg ctttgagatg acgacggcga actttgccct
     2041 ccagctgatc ccaactgtac ttcagcgcat gatttatgcc ctgcgggttg atatctttaa
     2101 ttctgaccgg aagccccgct ttacaagcag tgacataagc aataccgccg cccatcaagc
     2161 caccacctaa aattcccacg ctgtttaatg gcgcaggcgg cgcatcacta ccgggatctt
     2221 tcttcacttc cgtactggca aaaaagatac tacgcaaagc ttgcgattgt ggcgtcatcg
     2281 ccagttcgcc aaacgcccgt gcttcatcgt catagccact gctggtgccc tgcgctaatc
     2341 ccgtttcaac aacctccagg atgcgttctg tcgccggata attgccttga gttttgtgtt
     2401 ctgttttctt gccgaccatt ttgaacagca gcgcacgacc taacggcccc gccagaatac
     2461 gctcgcgtac aggtagaggg cgggaagatg ggcgatcctt cttcgccagc tcaacagcgg
     2521 cttccagcag aatggagtgc ggaacaacgt catccaccag ccccagcttt aatgcctgtt
     2581 tcgcccgaag ttgttttccg gtgaggatca tctctaatgc tgtgctgacg cctatcagac
     2641 gcggtaaacg ctgggtgccg cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac
     2701 cgagcaccgt tttaggatcg tcagtacaaa cgcgaccgtg gcacgccagc gccaactcca
     2761 gcccaccccc caggcaagcg ccatgaatag ccgcgataac cggaatgggc aaagcatgaa
     2821 tctccgccat caactgttgg ccctgccgcg ccagtgcttc cgcttcttgc gccgttttgc
     2881 agttgccgat catattgatg tctgctccgg cgataaagtt atccggttta gcggagataa
     2941 acaccacgcc gcgcaactct ttgttttcac ggagttgctt aataatggcg cgcacctgcg
     3001 aggcaaactc cgccttcagg gtattcattt tctcacccgg tacgtcgatg gtgataacgg
     3061 caatgttgtc aagacgaaca ttaagggtaa acgctgatgt catttccatt attccgcctc
     3121 cacaaccatt gccgcgccaa gcccacccgc agcacaggcg gtcactaaac caaatccacc
     3181 gccgcgacgg cgaagttcat gcaatgtctg ggtaatcatc cgtgcgccgg tcgccgcgaa
     3241 gggatgcccg taagcaatcg aaccgccaag cacgttaaat ttactatcgt ccacttcgcc
     3301 agtggcatgt gcacgcccca gtacatcacg agcaaaacgt tcactaccca gcaactgaat
     3361 attcgccagt gtctgagctg caaaggcttc gtgcatatcg atcaatgtca gatcgctcat
     3421 cgtcaaaccg gcacgctcca gcgccagcgg tgttgaccag gctggaccga gcaacatgtc
     3481 ctgccagaca tcaatcgcag taaatgcgta gctgcgcaga taccccagcg gcaccagccc
     3541 taattctttc gcccgggatt cagtcatcag gatcaccgct gccgcgccat cggtcagcgg
     3601 cgtactgttt gccgccgtta ccgttccgtg tttgcgatca aacgccgggc gcagctttgc
     3661 gtaatcggca agcgaggaat taccgcgaat attgttgtct tcgacaagcg gttgtttata
     3721 aggagggata aaggcagtca tcacctcttc tttgagtttt ccgtctgacc atgcctgagc
     3781 ggcacgctga tgcgaacggt gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt
     3841 tttcgccatt tgctctgcgg tgtcacccat ccgcaagccg gtagaatatt ctgctaccgc
     3901 aggcggtacg ggcattaagt cgcgcaaacg caggcgagag aagagtttca ggcgctggct
     3961 catgattcga gctttgttga aatcaaccag cacgcgcgcc agttttttac tgacgccaat
     4021 tggcaatacg gaagaggaat ctgccccacc ggcaatcccc gctcgaatag ttcccgccat
     4081 caggctttct gcgacgtttg caactgcctg gaaactggta gcgcaagcgc ggctgacgct
     4141 gtaagcatcg gtatgcacat tcattcccgt accgagaaca atttcacgcg caatgttggg
     4201 ggcttcaggc atttgtacga cctgaccaaa gaccagttgt tcaatcactt cagcggggat
     4261 ctcgctgcgt gccagcagtt cgcctaccac catcttccct aaatcaaccg cgggaatgcc
     4321 atgaaaagcc gtcgcctggc gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg
     4381 atcgccctgg cgggtaacca gcggtaaaac ctgacccata acactcccct gtaaaaaaat
     4441 aaataaagtg gtctgacctg atcatagtct taaccatttt tttacattta gccaagcgga
     4501 gaaaagggaa agtgggagct atgacacaga gagaaaagaa gaagagaaaa gaaaacgccc
     4561 ctgccagcaa ctgacagggg cgtacgtacc cgagaggaat taacgcagac ccagctggaa
     4621 gatcagcatt tcagcttcgc aggcaaaagt gaaatcgata tccaggcgca caccgtcaga
     4681 ctcttcagtg aaagtcgggg agattttgca cggttcggat tccacgctac gggctttctc
     4741 agtcagcgcc gccagcgttt gttctgcttc tgcgcggttt gcaaacacgc ggctgtaaga
     4801 cgcggtgcag tcggagttgt ccataatggt gccaacatcc atacagcagc aaaccggggt
     4861 ttcatcagca ctacatttac tcatcgttga tttcctctgt atgtgcaccc aaggtgccag
     4921 ataaacgttg tggatatttt acgcttccag aaagtgctgc tccagttgtt aattctgcaa
     4981 aatcggataa gtgaccgaaa tcacacttaa aaatgatcta aaacaaaatt cacccgaatc
     5041 catgagtgcg ccacctccaa attttgccag ctggatcgcg tttcttagat catatttgaa
     5101 aaaagataga aacatacttg caacattcca gctggtccga cctatactct cgccactggt
     5161 ctgatttcta agatgtacct cagaccctac acttcgcgct cctgttacag cacgtaacat
     5221 agtttgtata aaaataaatc attgaggtta tggtcatgag ccagaaaacc ctgtttacaa
     5281 agtctgctct cgcagtcgca gtggcactta tctccaccca ggcctggtcg gcaggctttc
     5341 agttaaacga attttcttcc tctggcctgg gccgggctta ttcaggggaa ggcgcaattg
     5401 ccgatgatgc aggtaacgtt agccgtaacc ccgcattgat taccatgttt gaccgcccga
     5461 cattttctgc gggtgcggtt tatattgacc cggatgtaaa tatcagcgga acgtctccat
     5521 ctggtcgtag cctgaaagcc gataacatcg cgcctacggc atgggttccg aacatgcact
     5581 ttgttgcacc gattaacgac caatttggtt ggggcgcttc tattacctct aactatggtc
     5641 tggcaacaga gtttaacgat acctatgcag gcggctctgt cgggggtaca accgaccttg
     5701 aaaccatgaa cctgaactta agcggtgcgt atcgcttaaa taatgcatgg agctttggtc
     5761 ttggtttcaa cgccgtctac gctcgcgcga aaattgaacg tttcgcaggc gatctggggc
     5821 agttggttgc tggtcagatt atgcaatctc ctgctggcca aactcagcaa gggcaagcat
     5881 tggcagctac cgccaacggt atcgacagta ataccaaaat cgctcatctg aacggaaacc
     5941 agtggggctt tggctggaac gccggtatcc tgtatgaact ggataaaaat aaccgctatg
     6001 cactgaccta ccgttctgaa gtgaaaattg acttcaaagg taactacagc agcgatctta
     6061 atcgtgcgtt taataactac ggtttgccaa ttcctaccgc gacaggtggc gcaacgcagt
     6121 caggatatct gacgctgaac ctgcctgaaa tgtgggaagt gtcgggttat aaccgtgttg
     6181 atccgcagtg ggcaattcac tatagcctgg cttacaccag ctggagtcag ttccagcagt
     6241 tgaaagcgac ctcaacgagt ggcgacacgc tgttccagaa acatgaaggc tttaaagatg
     6301 cttaccgcat cgcgttgggt accacttatt actacgatga taactggacc ttccgtaccg
     6361 gtatcgcctt tgatgacagc ccagttccgg cacagaatcg ttctatttcc attccggacc
     6421 aggaccgttt ctggctgagt gcaggtacga cttacgcatt taataaagat gcttcagtcg
     6481 acgttggtgt ttcttatatg cacggtcaga gcgtgaaaat taacgaaggc ccataccagt
     6541 tcgagtctga aggtaaagcc tggctgttcg gtactaactt taactacgcg ttctgataac
     6601 gcgttcgctt ggataaagtc acctgctata caggtgactt taactccccc acttcaccga
     6661 aagtagtgtc ctcattgctt gcctcctgag ttttgcaaac agcctgatgg cagcttgccc
     6721 tttcaaatca gtaagtggta tccacagaca aacttgaagg gaaacgcata ttttcaggcg
     6781 ttctgctcgt ccttctcaaa gagtttactt ttctgcattt ccaggatact cccccccctg
     6841 gctattgtgc gctcatacac tcaaattaaa gataggttct aaataaatga gcgttttttg
     6901 atagtctatt tcattaggta atatatattt gtaacaaatc aatcaaaatg gaataaaatc
     6961 atgctaccat ctatttcaat caacaatacc agcgcagctt acccagaatc catcaatgaa
     7021 aataacaatg atgaagttaa tggattagta caagagttca aaaacctttt taatggtaag
     7081 gaaggaataa gcacctgtat taaacatcta cttgagctta taaaaaacgc catacgagta
     7141 aacgacgatc cttatagatt taatattaat aattcctcag ttacttatat tgatattgac
     7201 tccaatgata cagaccatat tactattggt atcgacaacc aagaaccaat agaattacct
     7261 gcgaactata aagacaaaga actcgtccgt actatcatta atgacaacat agttgagaag
     7321 actcatgata tcaataacaa ggaaatgatc ttcagcgcat taaaagaaat atatgatgga
     7381 gatcctggtt ttatcttcga taagatatca cacaaactca gacatacggt aacggaattt
     7441 gatgagagcg ggaaaagcga accaacggac ttatttacct ggtacggtaa agataaaaaa
     7501 ggcgactctc tcgctattgt aattaaaaat aaaaacggaa atgattactt atctctcggt
     7561 tactacgatc aggacgacta ccacattcaa agaggaattc gtattaatgg tgatagtctc
     7621 acccaatatt gtagtgaaaa cgccaggagt gcttcagcgt ggtttgaaag cagtaaagct
     7681 atcatggcag aatcatttgc aacaggttcc gatcatcagg ttataaacga gctcaacggg
     7741 gaaagactga gagaacctaa cgaagttttt aaacgtttag gtcgagcaat aagatataat
     7801 tttcaagtgg acgatgcaaa atttagacgc gataatgtaa aagaaataat ttctaattta
     7861 ttcgctaa
//
LOCUS       DAOFQD010000179         7819 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.179, whole genome shotgun sequence.
ACCESSION   DAOFQD010000179 DAOFQD010000000
VERSION     DAOFQD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.179"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1199
                     /gene="argD/dapC"
                     /locus_tag="QBF46_004370"
     CDS             <1..1199
                     /gene="argD/dapC"
                     /locus_tag="QBF46_004370"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDQ6676474.1"
                     /translation="TRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEYVDFAGGIA
                     VTALGHCHSALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERVVFMNSGTE
                     ANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDGFGPKPADI
                     IHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQHQALLVFD
                     EVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAFHPGSHGST
                     YGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSDIRGMGLLI
                     GAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEGMQRFAHAV
                     AKVIGA"
     gene            complement(1265..3355)
                     /locus_tag="QBF46_004371"
     CDS             complement(1265..3355)
                     /locus_tag="QBF46_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDQ6676475.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSHKLRDSKA"
     gene            complement(3406..4038)
                     /gene="crp"
                     /locus_tag="QBF46_004372"
     CDS             complement(3406..4038)
                     /gene="crp"
                     /locus_tag="QBF46_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDQ6676476.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            4340..4744
                     /locus_tag="QBF46_004373"
     CDS             4340..4744
                     /locus_tag="QBF46_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDQ6676477.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            complement(4799..5668)
                     /locus_tag="QBF46_004374"
     CDS             complement(4799..5668)
                     /locus_tag="QBF46_004374"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDQ6676478.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(5722..5940)
                     /locus_tag="QBF46_004375"
     CDS             complement(5722..5940)
                     /locus_tag="QBF46_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDQ6676479.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            complement(5934..6956)
                     /locus_tag="QBF46_004376"
     CDS             complement(5934..6956)
                     /locus_tag="QBF46_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDQ6676480.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            complement(6956..>7819)
                     /locus_tag="QBF46_004377"
     CDS             complement(6956..>7819)
                     /locus_tag="QBF46_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDQ6676481.1"
                     /translation="KSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADESP
                     IQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPNLL
                     LLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPFDG
                     DLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIARLE
                     KEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQEQ
                     LEQMLLEGQSN"
BASE COUNT         1762 a         2021 c         2121 g         1915 t
ORIGIN      
        1 ttacacgcgc gactttcgat gaagtgatcc tgccgattta tgctccggca gagttcattc
       61 cggtaaaagg tcagggcagc cgaatctggg atcagcaagg caaggagtat gtcgatttcg
      121 cgggtggcat tgcagttacg gcgttgggcc attgccattc tgcgctggtg aacgcgttaa
      181 aaacccaggg cgaaactctg tggcatatca gtaacgtttt caccaatgaa ccggcgctgc
      241 gtcttgggcg taaactgatt gaggcaacgt ttgccgaacg cgtggtgttt atgaactccg
      301 gcacggaagc taacgaaacc gcctttaaac tggcacgtca ttacgcctgt gtgcgtcata
      361 gtccgttcaa aaccaaaatt attgccttcc ataacgcttt tcatggtcgc tcgctgttta
      421 ccgtctcggt gggcgggcag ccaaaatatt ccgacggctt tgggccgaaa ccggcagaca
      481 tcatccacgt tccctttaac gatctccacg cagtgaaagc ggtgatggat gatcacacct
      541 gtgcggtggt ggttgagccg atccaaggcg agggcggcgt gacggctgcg acgccagagt
      601 ttttgcaggg cttgcgcgag ctgtgcgatc aacatcaggc attattggtg tttgatgaag
      661 tgcagtgcgg gatggggcgg accggcgatt tgtttgctta catgcactac ggcgtgacgc
      721 cggatattct gacctctgcg aaagcgttag gcggcggctt cccgattagc gccatgctga
      781 ccacggcgga aattgcttct gcgtttcatc ctggttctca cggttccacc tacggcggta
      841 atcctctggc ctgtgcagta gcgggggcgg cgtttgatat catcaatacc cctgaagtgc
      901 tggaaggcat tcaggcgaaa cgccagcgtt ttgttgacca tctgcagaag atcgatcagc
      961 agtacgatgt gtttagcgat attcgcggta tggggctgtt gattggcgca gagctgaaac
     1021 cacagtacaa aggtcgggcg cgtgatttcc tgtatgcggg cgcagaggct ggcgtaatgg
     1081 tgctgaatgc cggaccggat gtgatgcgtt ttgcaccgtc gctggtggtg gaagatgcgg
     1141 atatcgatga agggatgcaa cgtttcgccc acgcggtggc gaaggtgatt ggggcgtaaa
     1201 taagacgcat caggcaaaag atgtggatgc ggcgtgaatg ccttatccga tggtttaacc
     1261 tgtattacgc cttcgaatcc cgcaacttgt gactcagcca aatcccgtga tgcggtcgct
     1321 gacgccatgc catcgacgaa atggtgtgca tggtgttcag atgaccaata acccgctgta
     1381 aatgctgctc cagcgtacct gccgcgcctt cgtgcggctg catctccggc gaatccatga
     1441 tattggcatc gccagaactc cccggttcgt catattccag tcgctgctga caacgctgaa
     1501 tggcgatttc acaagactgt aaatactctt gtgccagttc aggtggcaat gcccggtgtt
     1561 cccgcgccag cgtggtcatg gcattaatat gctcaacaat aaactggctg tgcgttaccc
     1621 acagtttcat atctgccaga taatggctgt taaacgccgg ttcctgcatc gcctgattca
     1681 atgagttata cagagtgtta tgtgcctgat ttacccgcat tcgctgccag gccagtggcg
     1741 taggttgcgg atcctcgcta agaatcaagc gaatcgcttc ctgataggct tctaaagcgt
     1801 catgggcgtt tttacgcaat aacccgctct gccactgcgg ccacagccag acagtaccgc
     1861 cgaaagcaat taaacaacca ataatggtat cgataagacg cggaaggatg tattgctcgc
     1921 cgttcaacca caatagttgc agggtataca ctgcggtaat agtaaaaccg accgtcgccc
     1981 agccgtagtt tttgcgcaat atcaggtagc tggcaagggt ggtaatcagc atcaaggtca
     2041 gggtgtaacc ttcgggaatt ttaaagtgca gcgccacgcc cgcaatgatt aacccgacca
     2101 cggttcccac ggagcgattc acaatcctca gacgggttgc gccatagcca ttttgcgtca
     2161 ccagcaatac cgtcatcagg atccagtacg acttcggcag atgcagcgcg gtgcccatca
     2221 ggctagcaac gcttaacatc acactgagtc gtccggcatt gcgtagcgcc ggagacttta
     2281 gtgacaaata acttttcagc gccggaagta acggcattcg ccgctgttta tcggccagta
     2341 agtcacgggc atagagcggt ttttgggtgc gcagcacgcg ggcgatgcgg ctgaaatgcc
     2401 aatagcagaa ttgcccaacc ggattatccg ggtgttggcg ggcgattttt tccagtgcgc
     2461 caatttgctt ttccatcgta aaacgcgttg gcaggcgatg gtaaagaatg tcatcagcca
     2521 gcacgcgcag gcgagcggca acggtttgcg cattccagcg gataacttct tccgcatggc
     2581 tacgctcgac cagtttttgc acctcttccg gctgatgcaa actgaccgaa atatgttcct
     2641 gcaaatccag cgcctcctgg aaaatacgca gcatccgctt gtagtcagta ttattttgcg
     2701 cggaaagcat atgcatttgc tgatagcact gggtaattag atcgaccgct ttttgctggc
     2761 gcaccagcag cggcggcagc gctttttcag ggtcggtgtg ctgggtaagc aggctgtatt
     2821 tggcttcaca ataatctgcc agttcacggt aaagcagact tagtgactcg cgcagcggct
     2881 gttcgcgcca gatccagaac caaaaccagt taaacaatcc gtaccagaga gtgcccaacg
     2941 catagatgag caatggttcc cagaccggca tgtatcccgc cagactgagg gtaaaaatgg
     3001 cggcgagcag cgatgcggga agcaattttg cgtgcaatgg ccccagctca gcagtgacgc
     3061 caagtaccag cgttaatccg gtcagcaaaa agggcagggg aacatctttt gccagtagta
     3121 gctgtgtcag taagctacag gtggcaaaca gcgacgcacc aatgattaag cgtttgaaaa
     3181 aacgtttatg aggcgtatca aggcccgcaa tattgcaaca ggcaggaacg agggagaaga
     3241 gcagaccgaa tcgtaattcg ccaagcatta acccaacggc cacgggcaaa catagcacca
     3301 gcgtttgtcg tagtgcatag ttaatatcgg ggtgataaat cagtctgcgc cacatcgggg
     3361 gaaacaaaat ggcgcgctac caggtaacgc gccactccga cgggattaac gggtgccgta
     3421 aacgacgatg gttttaccgt gtgcggagat caggttctga tcttcaagca tcttcagaat
     3481 gcgtcccacg gtttcacgag aacagccgac aatctgaccg atttcctgac gggtaatttt
     3541 gatttgcata ccgtccgggt gagtcatagc gtctggttgt tttgccagat tcagcagagt
     3601 ctgtgcaatg cggcccgtca cgtcgaggaa cgccaggttg cccactttct ctgaagtgac
     3661 ttgcagacga cgcgccatct gtgcagacag acgcatcaga atgtccgggt ttacctgaat
     3721 caattggcga aattttttgt acgaaatttc agccacttca caggcggttt tcgcacgtac
     3781 ccatgcgcta cgttcctggc cctcttcaaa caggcccagt tcgccaataa aatcaccctg
     3841 attcagatag gagaggatca tttctttacc ctcttcgtct ttgatcagca ctgccacaga
     3901 gcctttaacg atgtagtaca gcgtttccgc tttttcaccc tggtgaataa gcgtgctctt
     3961 ggatgggtac ttatgaatgt ggcaatgaga caagaaccat tcgagagtcg ggtctgtttg
     4021 cggtttgcca agcaccatgc gcggttatcc tctgttataa gctttctcca gagccagata
     4081 acgccgctgt ctctggattg ccgaaatatg cttcccgcta cctgggaagg ggctatcaac
     4141 tgtactgcac ggtaatgtga cgtcctttgc atacatgcag tacatcaatg tattactgta
     4201 gcatcctgac tgttttagca tagctttcgc tttgtgtctc ctggtgtctc gcttcagcat
     4261 gacccaggtc gccttccgtt gcgcgatttg gttagtacgc gtactctgtc aggaaaattg
     4321 acgcagtgga gtagcaaaaa tgcaagcgcg agtgaagtgg gtcgaagggt taacttttct
     4381 gggcgaatcc gcctctggtc atcagatttt aatggacggc aactcaggcg ataaagcacc
     4441 aagtccgatg gaaatggtgt tgatggcggc gggtggttgc agtgccatcg atgtggtttc
     4501 gatcctgcaa aaagggcgtc aggatgtggt cgattgtgaa gtaaaattga cctctgaacg
     4561 ccgcgaagag gcaccacgcc tgtttacgca cattaatctg cattttatcg tcaccggtcg
     4621 cgacctgaaa gacgcagcgg ttgcgcgtgc ggttgatctc tctgccgaga aatattgttc
     4681 agtggcgttg atgctggaaa aagcggtgaa tattactcac tcgtatgaag tggttgccgc
     4741 gtgacttaac tatcccgttg tgaagccgga tagtatttta tccggcgtaa tagcagagtt
     4801 actcgatttt ctttccttcc atcaatcgtt gcaccagcgg cagcataatt aattccattg
     4861 ccagacccat tttgccgccc ggtaccacta acgtattgat gtgggaaatg aatgagcctt
     4921 gcaacatcgc cagcagccag gggaaatcga tcccttccag attacgaaaa tggatcacca
     4981 caaagctttc atcgagcgac gggatacctt ttgccgcgaa cgggtttgaa gtgtcgacgg
     5041 tgggaacgcg ctggaagtta agatgggtgc gggaaaactg cggtgtgatg tagttgatat
     5101 agtcttccat tgaacgcact actgagtcca tcactgcttc tcgtgagtgc ccgcgctcgc
     5161 tggtgtcgcg gatcagtttt tgaatccact caaggttaac gataggcacc acgccgacca
     5221 gtaagtccac atgctgcgca acgttatgct gtggcgtgac tacgccgccg tgtaaacctt
     5281 cataaaacag tacatcagtg ggttccggta aaggttgcca gggggtgaat gtccccggta
     5341 cctgattcca cggtacggct tcgtcgtagg tatgcagata tttgcgagat ttccctttgc
     5401 cgctctgacc gtattcaatg aaggtttgtt ccagcaggcc gaagtcgtta gcctcggggc
     5461 cgaagtagct gatatgccgc ccggcgtcgc gcgctttgcg gatcgccatg tccatttccg
     5521 ggcgggtgta acggtgaaaa ctgtcgcctt ccacctcagc tgcatgcaga tttaactgcg
     5581 cgaatatttt acgaaacgcg aggctggtgg tggtggtccc cgcgccgctg gatcctgtta
     5641 ccgcaatgac cggatgtttg gcagacatag taactccatg acttagctat cattattatc
     5701 tcgtccgccg acggttgcag gttattcgcg aaactgactg cgcggcataa tattgaccgt
     5761 ttcgtgcagc tccgaccata ccagcaccgc ttctccgcac tgtagctggc gtttgacgtc
     5821 ggcgactttc tgttcaagtg tacgctcatg ttcaccataa tcggtgcctt cacgtaacac
     5881 aaagctttca attaaatttt ccagcgtttc gggggagagg tcttgccacg gaatcaacat
     5941 gatttcgcct ccagatacgt tgttaaccag tcaggaatgc gtgactccag ccacatttgc
     6001 ggatgaagta atgtaccgcc aataaagcca acatgaccgc catgttcagt cagttgatac
     6061 tccacctgcg gggggagact ttccggtttc gggatcacct gatgatccat aaacggatcg
     6121 tctttggcgt gaataatcag cgtcggtttg gcgatccggt tcagcatcgg catggcgcta
     6181 cactgacgat aatagtcgat agcgtcggcg tagccgtgaa ttctggcggt gatgagatcg
     6241 tcaaattcac ggatgcgacg taccgatttt aactgcgcga gattaatcgg cagcgttccg
     6301 gggtaggctg ccagcttgcg cgcggcattg gcttttaaca ggttcagcaa gtaacgctga
     6361 taaacgcggg aaaagccctt ttccatatga tagctacagg cttccagcat aaacggcgca
     6421 gagacaatca ccgccgcatc aaccgggaga tcattgcctt cctttgccag caaacaggcc
     6481 agcatattac cgccgagcga atagccgacg gcagccgttg gcgcatgacc aaattcgcgt
     6541 tgcagccagc gtaaaaacca actggcgtct tcggtttcgc ccgaatggta aatgcggtgc
     6601 atacggtttg gttcaccgct gcatccgcga aaatgcatca ccacgcccag ccagccgcgc
     6661 ttttgcgccg cctcaaccag accgtgggcg taagggctgt tgagactgcc ttccagcccg
     6721 tgaaacacca ccagacgagg tttatgttgc gcctgtgcag ggttttcact ccacgcaaga
     6781 tcgacaaaat cgccgtcggg caactccagc cgctgccagt acggggtgaa tttcacctga
     6841 cgacgaaaca gacgcggcag catggtttgc agatgacaat tgctaaagcc gcgcatgggg
     6901 atgaattcag cactgctgct gaattcattg gcatcggtcg tcgttatctg cgccatcagt
     6961 tgctttggcc ttccagcagc atctgctcaa gctgctcctg ggcttccagc catgccattt
     7021 cgcactcttc caggccggat ttggcgctgg cttgctgttg caggcaggcg gtcaactccg
     7081 ctttacggct ctggtcatac agttcgctgt cgccgagttt ctcttccgcc tgcgccagtt
     7141 gcgcgttcag cttctccatc tctttttcca gacgggcaat ctctttacgc agtggctggg
     7201 tttgcgcacg cagctcagct tcccgacgct tctgatcttt acgtgcctgg gcgctgttcg
     7261 cattctcttt tggcgcttcg tcggtctggt tttcctgctt ttgtacgtcg ctcaaccact
     7321 gttgataatc ttccagatcg ccgtcgaacg gttcgacttt acgatcgtga accaggtaga
     7381 gatcgtcagt ggtggaacgc agcaaatgac ggtcgtgcga aacgacaacc agcgcgcctt
     7441 caaactcgat taatgcttcg gtgagtgcct gacgcatgtc gaggtcaagg tggttagtcg
     7501 gttcgtcgag cagcagcaga ttcggccgct gccagacaat taatgccagc accaggcggg
     7561 ctttttcccc accggagaag cggcgcgttt cttcggttac tttatcgccc tggaaaccaa
     7621 agccgccgag gtagtcacgc agtttttgtt ccagctcctg cggcgctaaa cgtgccagat
     7681 gttgaatagg tgattcgtcg gcgcgcaggt attcaagttg atgctgggcg aagtagccga
     7741 gtttgatccc tttcgccaga ccaatttcac cgctgactgg cgcaagttca ccggctaaca
     7801 gtttgattaa tgtcgattt
//
LOCUS       DAOFQD010000180         7765 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.180, whole genome shotgun sequence.
ACCESSION   DAOFQD010000180 DAOFQD010000000
VERSION     DAOFQD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.180"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            59..148
                     /gene="kdpF"
                     /locus_tag="QBF46_004378"
     CDS             59..148
                     /gene="kdpF"
                     /locus_tag="QBF46_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDQ6676482.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            148..1821
                     /gene="kdpA"
                     /locus_tag="QBF46_004379"
     CDS             148..1821
                     /gene="kdpA"
                     /locus_tag="QBF46_004379"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDQ6676483.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1844..3892
                     /gene="kdpB"
                     /locus_tag="QBF46_004380"
     CDS             1844..3892
                     /gene="kdpB"
                     /locus_tag="QBF46_004380"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDQ6676484.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWITVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAEDKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3901..4473
                     /gene="kdpC"
                     /locus_tag="QBF46_004381"
     CDS             3901..4473
                     /gene="kdpC"
                     /locus_tag="QBF46_004381"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDQ6676485.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4466..7150
                     /gene="kdpD"
                     /locus_tag="QBF46_004382"
     CDS             4466..7150
                     /gene="kdpD"
                     /locus_tag="QBF46_004382"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDQ6676486.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEDENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7147..>7765
                     /gene="kdpE"
                     /locus_tag="QBF46_004383"
     CDS             7147..>7765
                     /gene="kdpE"
                     /locus_tag="QBF46_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDQ6676487.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQD"
BASE COUNT         1571 a         2037 c         2336 g         1821 t
ORIGIN      
        1 tctttgcagc cagaattcta cccttccggt atcactttta ggccactgga ggtgcactgt
       61 gagtgcaggc gtgatagccg gcgtattgct ggtgttttta ttactgggtt atctggttta
      121 tgccctgatc aatgcggagg cgttctgatg gctgcgcaag ggttcttact gatcgccacg
      181 tttttactgg tgttaatggt gctggcgcgt cctttaggca gcgggctggc ccggctgatt
      241 aatgacattc ctcttccagg tacagcgggc gttgaacgta tactctttcg tttacctggc
      301 gtctctgacc atgagatgaa ctggaagcaa tatctttgtg ccattctcgg cctgaacatg
      361 ctggggctgg cggtgctgtt ttttatgttg ctcggtcagc actatctgcc gcttaatcca
      421 cagcagttgc cagggctgtc gtgggatctg gcgctgaata ccgccgtcag ctttgtcacc
      481 aataccaact ggcaatctta tagcggtgaa accacgttga gctatttcag ccagatggcg
      541 ggcttaacgg tgcaaaactt tctttctgcc gccagcggga ttgcggtgat ttttgccctc
      601 atccgtgcgt ttacccgcca gagcatgagc acgctcggga atgcctgggt cgatctgcta
      661 cgcatcacgt tatgggtgct agtccctgtg gcgttgttga ttgcactgtt ttttattcaa
      721 caaggtgcgc tgcaaaactt tctgccttat caggctgtga ataccgttga aggagcgcaa
      781 cagctgttac ccatggggcc tgtagcttct caggaagcga tcaagatgct cggtactaac
      841 ggcggtggct tctttaatgc caactcgtcg catccgtttg aaaacccaac cgcactgacc
      901 aacttcgtgc agatgctggc gatcttcttg atcccaacgg cgctgtgctt tgcctttggt
      961 gaagtgacgg gcgatcgccg ccaggggcgc atgttgctgt gggcgatgtc agtgattttt
     1021 gtcatctgcg taggcgtggt gatgtgggca gaagttcagg gtaatcctca tctgctggcg
     1081 ctcggcgcag acagcagcat caatatggaa ggtaaagaga gccgtttcgg cgtgctggtc
     1141 agtagcctgt ttgcggtcgt gactacggcg gcttcctgtg gcgcggtgat tgcgatgcat
     1201 gattcgttta ccgctctcgg tggcatggtg ccgatgtggc tgatgcaaat tggtgaagtg
     1261 gtgttcggcg gtgtcggttc tggtctttac ggcatgatgc tgtttgtcct gctggcggtg
     1321 tttattgccg ggctgatgat tggtcgtaca ccggaatatc tgggtaaaaa aatcgacgta
     1381 cgtgagatga aactgactgc actggcgatt ctggtcaccc cgacgctggt gctgatgggc
     1441 gcggcgctgg caatgatgac cgacgccgga cgtagcgcca tgctcaaccc tggcccgcat
     1501 ggttttagcg aagtgctgta cgccgtgtca tccgccgcta acaacaacgg cagcgccttt
     1561 gccggattaa gcgccaactc tccgttctgg aactgtttac tggcgttctg catgtttgtc
     1621 ggtcgcttcg gggtgattat cccggtgatg gcaattgccg gttcgctggt gagtaaaaag
     1681 agccaacccg ccagctccgg cacgctgcca acgcacggcc cgctgtttgt tggcctgtta
     1741 atcggcaccg tgttgctggt tggcgcactg acctttatcc ctgccctggc gcttggtccg
     1801 gtggcggaat atctctcctg atgatattga gtgagcactg aatatgagtc gtaaacaact
     1861 ggcgctattc gaaccaacac ttgtcgttca ggcgctgaaa gaagcggtga aaaaattaaa
     1921 cccgcaggcg caatggcgca atccggtgat gtttatcgtc tggatcggca gtctgctgac
     1981 cacctgtatt agcatcgcga tggcaagcgg tgcgatgcct ggcaatgcgc tgtttagcgc
     2041 ggccattagc ggctggctgt ggatcaccgt actgttcgct aatttcgccg aggcgctggc
     2101 agaaggccgc agtaaagcgc aggccaacag tctgaaaggg gtgaaaaaaa ctgcctttgc
     2161 ccgcaagctg cgtgagccga aatatggcgc tgcggaggac aaagttcccg ccgatcaact
     2221 gcgtaaaggc gatatcgtac tggtagaagc tggcgatatt atcccctgcg atggtgaagt
     2281 cattgaaggg ggcgcatcgg tcgatgaaag cgccatcacc ggggaatcgg caccggtgat
     2341 ccgtgaatcc ggcggcgatt ttgcctccgt caccggcggc acgcgtattc tttctgactg
     2401 gctggtgatt gagtgtagcg ttaaccccgg cgagacattt ctggatcgga tgatcgcgat
     2461 ggtggaaggc gcacagcgac gtaaaacgcc gaacgagatt gccctgacca tcctgctgat
     2521 tgccctgact atcgtctttt tactggcaac cgccacgctg tggccgtttt ccgcgtgggg
     2581 cggtaatgca gtcagcgtaa cggtactggt ggcgctgctg gtctgtctga tcccaaccac
     2641 tattggcggc ctgttgtcag cgatcggcgt cgccgggatg agccggatgc taggcgcgaa
     2701 tgtgattgcc accagcggac gtgcagttga agcggcaggt gacgttgacg ttctgctact
     2761 ggataaaacc ggcaccatca cactcggtaa ccgtcaggcg tcggagttta tccctgcgca
     2821 gggcgtggat gaaaaaacgc tggctgacgc cgcacaactg gcttcgctgg ctgatgaaac
     2881 gccggaaggc cgcagtattg tgatcctcgc caagcaacgt tttaacctgc gcgagcgcga
     2941 tgtgcagtcg ctccatgcca cctttgtacc gtttactgcg caaagccgga tgagcgggat
     3001 caacatcgac aaccgcatga tccgtaaagg ttctgtcgat gccattcgcc gccatgttga
     3061 ggctaacggc ggtcacttcc ctgccgatgt tgatcaaaaa gtcgatcagg ttgcgcgtca
     3121 gggggccacg ccgctggtgg tggtggaagg ttcccgtgtg ctgggcgtta ttgcgctgaa
     3181 agatatcgtc aaaggcggta ttaaagagcg cttcgcccag ctgcgcaaaa tgggcattaa
     3241 aacggtgatg attaccggcg ataaccgtct gactgccgcc gccattgctg cggaagcggg
     3301 cgtcgatgat tttctcgccg aagcgacacc ggaggccaag ctggcattga ttcgtcagta
     3361 tcaggcggaa ggccgtttgg tggcgatgac cggcgacggc accaacgatg ctccggcgct
     3421 ggcgcaggca gatgtcgcgg tggcgatgaa ctccggcacc caggcggcga aagaggcggg
     3481 caatatggtc gatctcgact ctaacccgac caagttgatc gaggtggtgc atattggcaa
     3541 acagatgctg atgacccgtg gctcgctgac caccttcagc attgccaacg atgtggcgaa
     3601 atacttcgcc attattccgg cggcgttcgc ggcaacgtat ccgcagttaa atgcgctgaa
     3661 catcatgcgc cttcattcgc ccgactccgc cattctcagt gcggtcattt tcaacgcctt
     3721 gattatcgtc tttttgattc ctctggcgtt aaaaggcgtg agttataaac cgcttaccgc
     3781 ttctgccatg ttgcgccgta acttatggat ttacggtctg ggtgggctgc tggtgccgtt
     3841 tattggtatc aaagtcattg atttactgct gaccgtttgc ggtctggtgt gaggtttacc
     3901 atgcgtggct tacgtccggc attatcgaca tttatctttc tgttattgat tactggcggc
     3961 gtttacccgc tgctgaccac cgtgctgggg caatggtggt ttccctggca ggccaacggt
     4021 tcgttgattc gtgaaggtga tacggtgcgg ggttcggcat taatcgggca gaattttacc
     4081 ggcaacggct attttcaggg tcgcccgtcg gcaacggcag aaatgcccta taatccacag
     4141 gcttctggcg ggagcaatct ggcggtcagt aaccctgagc tggataaaca aatagccgca
     4201 cgcgttgctg cattacgggc cgctaacccg gatgccagta cgaacgttcc ggttgaactg
     4261 gtgacggcat cggcaagcgg gctggacaat aatatcaccc cgcaagcggc ggtctggcaa
     4321 atcccacgcg tggcgaaagc gcgtaatctc agcgttgaac agctcacgca actgatcgca
     4381 aaatacagcc aacaaccgct ggtgaaatat atcggccagc cggtcgttaa cattgttgaa
     4441 ctcaatctgg cgctggataa acttgatgaa taacgaaccc ttacgtcccg accccgatcg
     4501 tctgctggaa caaactgccg cgccgcatcg ggggaagctg aaagttttct tcggtgcctg
     4561 tgcaggcgtc gggaagacct gggcgatgct ggcagaagcc cagcgactgc gggcgcaagg
     4621 gctggatatt gtggttggcg tggtagaaac ccatgggcgg aaagataccg ccgccatgct
     4681 tgaagggctg gctgttctgc cgcccaaacg ccaggcgtac cgtgggcggc atatcagcga
     4741 gtttgatctc gatgccgccc tcgcccgccg tccggcgctg atcttaatgg acgaactggc
     4801 gcacagtaat gcgccaggtt ctcgtcatcc taaacgctgg caggatatcg aagaactgct
     4861 ggaagctggc attgatgttt tcacaaccgt caacgttcag catctggaaa gtctgaatga
     4921 tgtggtcagc ggcgtcaccg gaattcaggt acgggaaacc gtgcccgatc cttttttcga
     4981 tgccgccgac gacgtagtgc tggtggactt gcccccggac gatctgcgcc agcggctgaa
     5041 agaaggcaaa gtctatattg ccgggcaggc ggagcgcgcc attgaacatt ttttccgcaa
     5101 aggcaatctg atcgccctgc gtgaactggc actgcgccgt actgccgatc gcgtagatga
     5161 gcaaatgcgc gcctggcggg ggcatcctgg tgaagagaaa gtgtggcaca cgcgcgacgc
     5221 gatcctttta tgcatcgggc ataacaccgg cagtgagaaa ctggtccgcg cggcggcgcg
     5281 gttggcctcg cgcctgggta gcgtctggca cgcggtgtat gttgaaaccc ctgccctgca
     5341 tcgcttaccg gaaaaaaaac gtcgggcaat tctcagcgcc ttacgtctgg cgcaggagct
     5401 gggcgcggag acggcaacac tttctgatcc agcggaagaa aaagcagtag tgcgttatgc
     5461 ccgtgaacat aatctcggca agattattct cggtcgcccg gcctcgcgcc gctggtggcg
     5521 tcgggaaacg tttgctgacc gactggcgcg catcgccccc gatctcgatc aggtgctggt
     5581 cgcgcttgat gaaccacccg cccgcacgat taacaacgcg ccggatagcc gctcttttaa
     5641 agacaagtgg cgggtacaga ttcagggatg cgtggttgcc gccgcgttat gcgccgttat
     5701 cactttaatt gccatgcagt ggctgatggc gtttgatgcc gccaacctgg tgatgctgta
     5761 tctgcttggc gtggtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgctac
     5821 cgtcattaat gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgctgt
     5881 ctctgatgtg caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg
     5941 gaaccttact gcgggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac
     6001 acggcactta tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc
     6061 cgccaccagc gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc
     6121 cgatgacaac ggtaaattgc agccgttaac acatccgcaa ggaatgacgc catgggacga
     6181 tgccatcgcg cagtggagtt ttgataaagg cctgcctgcg ggtgcgggca ccgacacgtt
     6241 acccggcgtg ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acggactggt
     6301 ggtggtggaa ccggggaatc tgcgtcagtt gatgatcccg gagcagcagc gcctgctaga
     6361 gacgtttacg ctgttagtcg ccaatgcgtt tgagcggctg acgctgaccg ccagtgaaga
     6421 acaggcgcgg atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cggcgctttc
     6481 gcatgattta cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga
     6541 tctggcaagc gaaggatcgc cccacgcccg ccaggccagc gagattcgtc agcatgtgct
     6601 gaacactacc cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt
     6661 taatttgaag aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgct
     6721 ggaaccgggt ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca
     6781 cgttgacggg ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata
     6841 tgcgggtgcg caggccgaaa ttggtatcga tgcccacgtt gaggacgaaa atctacaact
     6901 ggatgtctgg gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa
     6961 gtttgctcgc gggaataaag agtcggcagt accgggtgta gggcttggac tggcaatttg
     7021 tcgggcgatt gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg
     7081 tgcctgtttt cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga
     7141 ggatatgtga caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc
     7201 acggcgctgg agggcgacgg gatgcgcgtc tatgaggccg aaacgctgca acgcggcttg
     7261 ctggaagcgg ctacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt
     7321 gatgggattg agtttatccg cgacctgcgc cagtggagcg cggtgccggt gattgtgctt
     7381 tccgcacgca gcgaagagag cgacaaaatc gccgcgctgg atgccggagc ggatgattat
     7441 ctgagtaagc cgtttggcat tggcgaattg caggcccgtc tgcgcgtcgc attacgccgc
     7501 cactctgcca ccaccgcgcc cgatccgctg gtgaaatttt ccgatgttac cgtcgattta
     7561 gccgcccgcg tgatccatcg cggtgaggaa gaggtgcatc tcaccccgat tgagtttcgc
     7621 ctgctggcgg tgctgctgaa taatgccggg aaagtgctca cccagcgcca gttactcaat
     7681 caggtgtggg ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat
     7741 ctgcgacaaa aactggaaca ggatc
//
LOCUS       DAOFQD010000181         7764 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.181, whole genome shotgun sequence.
ACCESSION   DAOFQD010000181 DAOFQD010000000
VERSION     DAOFQD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.181"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1252)
                     /locus_tag="QBF46_004384"
     CDS             complement(<1..1252)
                     /locus_tag="QBF46_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676488.1"
                     /translation="MQTAATLPPAGGTALAQPQPLPPLRDELKIHPAGNNRDGSPAWH
                     LADPVRNLYFRLGWLELEMLRRWPLGAPQAIADSISQETTMTVDEQDVLQFLGFLQQQ
                     QLLRRGAFKARGSLWRRILHGYLFIRLPLVRPEKALRRMLPWVEWLFSAPFLLLTAVA
                     ALAGVVLAARQWDNVEAALHGALSWNGALAFAVALILSKCWHELGHAFMATRYGVRVG
                     HMGVALLVMWPMAYTDTGESWKLSQSRHRLSIASAGIMAELMLAAWATLFWSFAPEGN
                     VKGALFFLATTAWVLTVAVNASPFMRFDGYFILSDTLDYPGLHERAGNWAKRWLRWHL
                     LGLEDPIPDNVSPAFARFLTLFAFATWVYRLLLFVGIALVVYNAFFKALGLVLFFIEI
                     VTFVVQPMFRELKIWRARRREIAPA"
     gene            complement(1254..2603)
                     /locus_tag="QBF46_004385"
     CDS             complement(1254..2603)
                     /locus_tag="QBF46_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDQ6676489.1"
                     /translation="MSIALFYSLAARVKAARSPEELGFVMCNDTRSLVEYRQAALLAV
                     SATGRAQLAAHSGLSDTDRNTPYALWLASVACDIAPRCAALPETARVMPLSPEMLSES
                     LAREWDEWLPGHVWVLALTGPDGKLHALLLLARDTPWPTQLGVESPEYALLQLANLYG
                     YAWWALVARPSRLQRLLPATVRRRAGLVLLLLLLVMLIPIREYTLVPAEVISLNSEVI
                     AAPSDGVIHQMKVPPNTPVVAGQVLAELDDTTLKNRLAIAQAELATATVNMHQASQQA
                     IEDPNARAELALTEGKWREQKVQVASLQRELEKLSIRARSSGVFVYTDPDDWAGRPVQ
                     TGERIGLLADPTKLGVRAWAPAAESTNLNPHAAMTVFLKVAPLHPLTARLDYAGYETV
                     EAPNGVASYLLRGTVTDPSSAARIGLQGTARVSGDWSLLGYLIFRRPLATLRAWCGF"
     gene            complement(2600..3481)
                     /locus_tag="QBF46_004386"
     CDS             complement(2600..3481)
                     /locus_tag="QBF46_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDQ6676490.1"
                     /translation="MNNVNEGWRSVRALMLLTGLALCASAFAADPTPAAAGAVATPDA
                     AVAAAPESAPDATADNGAAPVRFLVMANQESPLSSVVAGRVSQVNVELGDTVKAGKLL
                     VTLDCSELKARRDAADAEYSAAQIKYEAKAKLQGLHSAGALEVGLAAADVNRTRSQIK
                     IFEAQLAQCRFTAPFDGRVARIYVKEGQGVGAGAQVIDLVSNGTRKARLNVPSGWTTW
                     LAPGAQLDAVVGETGKHYPMTVTHISGRVDAVSQTIEIEAEFGSDAQDVLPGMSGRAT
                     PVCPKAAAQCQSGTTNS"
     gene            complement(3496..5415)
                     /locus_tag="QBF46_004387"
     CDS             complement(3496..5415)
                     /locus_tag="QBF46_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDQ6676491.1"
                     /translation="MMTTLTAVTPTAVRSAPLLAPVAALVSALLLSGCGTIKPEPLTQ
                     DEIGARVSADRAAMYRDQQPVSGPLTLSDAMARALKYNLDYRLKMMETALSRGILDIS
                     QQDMLPKLMTDAGYRWRDNDSGGTSVGIEDRVVSLRPSTSEEREHLLADATFSWDVLD
                     FGMSYYRAKQQADNVNIAEERRRKVLQNIVQEVRDAYWRALGAQRLLSEAQPLAEEIQ
                     TALEKTREAERAGILPPVEGLEYQRALLDAVALLNQKRQEMQMAKSELAALLNLPSTT
                     DFTLQDVANPALSGVPSQLDTLEQMALERRPELREEDYKTRIDVLETKRQIASLFPNL
                     NLFGGISYDSNSYLYNNSWVQGGVGVSMNLFKLLSAPAISRTNDARLATDNARRMALS
                     MAVLTQVRVSVERYKLAVYDYQIAQESARVDQRLASISRAGSDNSLSSDLESLRTQAR
                     SIVSRFQEAASYAQAQSAYGRVLNSVGIDLLPEKVTSSDLPTLSREINQSLVAGEKQV
                     FTQSADAVVSRHPVSVTLGRLPAGVNKSAIYQSVTHVLAANQLVQGNGADSVKLQMNL
                     NLERASGSQRGVWEMAVYNAQGAQVAYQKYTSYLPNDVNTRSVSALAEAAALSVVSDL
                     HKAAGDTGVMTASNP"
     gene            complement(5492..>7764)
                     /locus_tag="QBF46_004388"
     CDS             complement(5492..>7764)
                     /locus_tag="QBF46_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HDQ6676492.1"
                     /translation="VSVVMTNTVPVADGEIVTTPEDTAIGGELLTNDRDPDGDPLHIA
                     GFTVGGQTAQPGDTVQLAGVGALTVNRDGSYRFTPVADWNGTAPVVTYTVSDGNDGGT
                     ATALLVITVTPVVDVKDDRATTHAGDPVTVDALGNDRFVNPDQAITGVTQGAHGSVAI
                     ENGQLVYTPNAGYVGQDTFTYTVTSGGVTETAQVTLEVTNTPPVAVADKASTLPETPV
                     SGNLLTNDRDADSDPLHVAEITVGGATYAPGDIITIPGQGTLVVNRDGSYLFTPASGW
                     SGFTPVLNYTLSDGNDGGTATGELRLLVNPVAEAWVKEAGLVDTASGAQTTTGAMAVL
                     SLEPVESLTIGGQTLTLAQLQALSAQAPVDIATPDGVLSLTGFQVDGEGRATLQYRFT
                     LTQAVNQPGESTTREEIRFSVNGQQTRAPGLLRVNILNDAPVAAADDNSIDQDRGQQA
                     ASGNVFSNDAIGADGAAAGGPVSAISSVNLNRAGAVGGVSLGEFGALTLDARGNYSYV
                     LNRSNSRVASLDANATLSEVFTYTITDADGNTSQAQLTIVIHGVTPPQSVRTGDQHFP
                     SYYTNYELSLDQPYSPGLFILPAIYGLYSDQFSRKVELNRKITELGRGMNDNGTPVLE
                     DGILFTRWVNTTLQRSVVNTFAATGIGSQLLGDHFSHFSLNKSVQPAPVLENAPERPP
                     LNERINERTTVQQERGEKTPDAKQVHAAAPGVVIVPQAAARPGAPSLAAQVDALARNR
                     VAAPEPVTVGGATPHR"
BASE COUNT         1261 a         2487 c         2625 g         1391 t
ORIGIN      
        1 gcgccggagc gatttcccgg cggcgcgcgc gccagatttt gagttcgcga aacatcggtt
       61 gcaccacaaa agtgactatc tcaataaaaa acagcaccag cccgagcgcc ttaaaaaagg
      121 cgttgtagac caccagcgcg atgccgacga acagcagtag ccggtaaacc caggtggcga
      181 aggcgaaaag ggtgaggaag cgggcaaagg cgggcgagac gttatccggg atcggatcct
      241 caagccccag caggtgccag cgcagccagc gcttcgccca gttgcccgcc cgttcgtgca
      301 ggccagggta gtccagcgta tcgctgagaa taaagtagcc gtcaaagcgc ataaacgggc
      361 tggcgttcac cgccaccgtc agcacccagg cggtggtggc gagaaagaac agcgcgcctt
      421 taacgttgcc ttccggcgcg aagctccaga acagcgtcgc ccaggccgcc agcatcagct
      481 cggccataat gcccgcgctg gcgatggaga gccggtggcg ggactgcgac agcttccagc
      541 tctctccggt atcggtgtag gccatcggcc acatcaccag cagcgccacc cccatatggc
      601 cgacgcgtac gccgtagcgg gtcgccataa aggcgtgccc cagttcgtgc cagcatttgg
      661 agaggatcag cgccaccgcg aaggccagcg ccccgttcca gctcagggcg ccgtgcagcg
      721 cggcctcgac gttatcccac tggcgcgcgg ccagaacgac gcctgccagc gcggcgacgg
      781 cggtcagcag tagaaacggc gcgctgaaaa gccactccac ccacggcagc atgcggcgca
      841 gggctttctc cgggcgcacc agcggcaggc ggataaacag gtagccgtgc agaatacgcc
      901 gccagagcga gccgcgcgct ttgaacgcgc cgcggcgcag cagctgttgc tgctgtaaaa
      961 agcccaggaa ctgcaacaca tcctgttcgt ccaccgtcat ggtggtctcc tggctgatgc
     1021 tgtcggcaat cgcctgcggc gcgccgagcg gccagcggcg cagcatctcc agctccagcc
     1081 agcccaggcg gaaatagagg ttgcgcaccg ggtcggccag atgccaggcg ggcgagccgt
     1141 cgcggttgtt gcccgccggg tgaattttta gctcgtcgcg cagcggcggc agcggctgcg
     1201 gctgcgcaag agctgtcccg cccgcaggcg ggagcgtcgc ggctgtttgc atatcagaat
     1261 ccacaccagg cgcgcagcgt cgccagcgga cgacggaaaa tcagataacc gagcaggctc
     1321 cagtcgccgg agacgcgggc cgtgccctgc aagccgatac gggcggcgct ggacgggtcg
     1381 gttacggtgc cgcgcagcag gtagctcgcg acgccgttcg gcgcttccac cgtctcgtaa
     1441 ccggcgtaat ccagccgcgc ggtcagcggg tgcagcgggg cgaccttaag aaatacggtc
     1501 atcgccgcgt gcgggttcag gttggtggat tccgccgcag gcgcccaggc gcgcacgccg
     1561 agcttcgtcg ggtccgccag caggccgatg cgctcgccgg tctgtaccgg acggcccgcc
     1621 cagtcgtccg ggtcggtgta gacaaacacg ccgctgctgc gggcgcggat cgacagcttc
     1681 tccagctcgc gctgcaacga ggcaacctgc accttttgct cgcgccattt gccttcggtc
     1741 agagccagtt ccgcacgggc gttcgggtct tcaatcgcct gctgagacgc ctgatgcata
     1801 ttgacggtgg cggtcgccag ctccgcctga gcaatggcga gacggttttt tagagtcgta
     1861 tcatccagct ccgccagcac ctgtccggcc acgaccggcg tgttcggcgg caccttcatc
     1921 tggtgaatca ccccgtcgct cggcgcggcg atcacttcgc tgttaagcga aatcacctcg
     1981 gcgggcacca gcgtgtattc ccgaatcggg atcagcataa ccagcagcag aagcagcaac
     2041 acgagaccag cgcgtcggcg caccgtggcg ggcagcagcc gttgcaggcg agaaggccgc
     2101 gccaccagcg cccaccaggc gtagccgtac aggttcgcca gctggagcag ggcgtattcg
     2161 gggctttcga cgccaagctg cgtcggccac ggggtatcgc gggccagcag cagcagggcg
     2221 tgcagcttgc cgtccgggcc ggtcagcgcc agcacccaga cgtggcccgg cagccattcg
     2281 tcccactcgc gcgccagcga ctcgctcagc atttccggcg acagcggcat cacgcgggca
     2341 gtttccggca gcgcggcgca gcgcggcgcg atgtcgcagg caacggaggc aagccacagc
     2401 gcgtaggggg tattgcggtc ggtgtcgctc aggccggagt gcgccgcaag ctgggcgcgc
     2461 ccggtggcgg aaacggcgag cagcgccgcc tggcgatact ccaccagcga gcgggtatcg
     2521 ttacacatca caaaacccag ctcttcgggg ctgcgcgccg ctttcacgcg cgccgcgaga
     2581 gaatagaaca gggcgatgct cacgagttgg tcgtcccgct ctggcactgc gccgccgctt
     2641 tcgggcagac tggcgtcgcg cggccgctca tgccgggcag tacgtcctgg gcgtcgctgc
     2701 caaattccgc ttcaatctca atcgtctggc tcacggcgtc cacgcggccg ctgatgtggg
     2761 tgacggtcat cgggtaatgc ttgccggttt cacccaccac cgcgtcaagc tgcgcgccgg
     2821 gcgcaagcca ggtggtccag cctgacggca cgttgaggcg cgccttgcgg gtgccgttgc
     2881 ttacaagatc gataacctgc gcgcctgcgc cgacgccctg gccctctttg acgtaaatac
     2941 gcgccacgcg gccgtcgaac ggcgcggtaa agcggcactg cgccagctgc gcttcaaaga
     3001 ttttgatctg gctgcgggtg cggttcacgt cggcggcggc aaggccaacc tccagcgcgc
     3061 ctgcggaatg cagaccctgc aatttcgcct tcgcttcata tttgatctgc gcggcgctgt
     3121 actcggcgtc tgccgcgtcg cgacgggctt tcagctcgct acagtccagc gtcaccagca
     3181 gtttgcctgc cttgaccgta tcgcccagct caacgttgac ctgcgacacg cgcccggcca
     3241 ccacgcttga tagcgggctt tcctggttgg ccatcaccag aaaacgcacc ggcgcggcgc
     3301 cgttgtcggc ggtggcgtca ggcgcgctct caggcgctgc ggcaacggcc gcatccggcg
     3361 ttgcgactgc ccccgccgca gcgggggttg ggtcggcggc aaaggcgctg gcgcacagcg
     3421 ccaggcccgt cagcagcatg agcgcgcgga cgctgcgcca gccttcgttc acgttgttca
     3481 tacctgttcc tgttattaag ggttacttgc cgtcatcacg ccggtgtcgc ctgccgcttt
     3541 atgcaggtcg gacaccacgg agagcgcggc ggcttctgcc agcgcgctga ccgaacgggt
     3601 gttgacgtca ttcggcagat agctggtgta tttctggtag gcgacctgcg cgccctgggc
     3661 gttatagacc gccatttccc acacgccgcg ctgcgagcct gacgcgcgtt caaggttgag
     3721 gttcatttgc agtttcacgc tgtcggcgcc gttgccctgc accagctggt tggcggccag
     3781 cacatgggtg accgactgat aaatcgcgct cttgttgacg cccgctggca ggcggcccag
     3841 ggtgacgctg accggatggc gggaaactac cgcgtcggcg ctctgggtga agacctgctt
     3901 ctcgcccgcc accaggctct ggttgatttc acgcgacagg gtcggcagat cgctgctggt
     3961 caccttttcc ggcagcaggt cgatgcctac cgagttcagc acgcggccat aggcggactg
     4021 cgcctgcgcg taggaggcgg cttcctggaa gcgggagacg atggagcgcg cctgggtgcg
     4081 cagggattcc aggtcgcttg agagcgagtt gtcggaaccg gcgcgggaga tgctcgccag
     4141 acgctggtcg acgcgggcgg actcctgggc tatctggtag tcgtacaccg cgagcttgta
     4201 gcgctcaacc gagacgcgca cctgagtcag caccgccatg ctcagcgcca tacggcgggc
     4261 attgtcggtg gccaggcgcg cgtcgttggt gcggctgatg gccggcgcgc tcagcagctt
     4321 gaacaggttc atggaaacgc ccacgccgcc ctgcacccag ctgttgttgt agaggtagct
     4381 gttggagtcg tagctgatgc cgccgaacag attcaggttc gggaacagcg aggcgatctg
     4441 gcgtttggtt tccagcacgt cgatgcgggt tttgtaatct tcttcacgca gctctgggcg
     4501 gcgttccagc gccatctgct caagcgtatc aagctggctt ggcacgccgg aaagcgccgg
     4561 attggcgacg tcctgtaagg tgaagtcggt ggtggacggc aggttcagca gggcggccag
     4621 ctcgcttttc gccatctgca tctcctggcg cttctggttc agcagcgcca ccgcgtcgag
     4681 cagggcgcgc tggtactcca ggccctcgac cggcggcaga atgcctgcgc gctccgcttc
     4741 gcgggttttc tcaagcgcgg tctggatctc ttcggccagc ggctgcgctt cgctcagcag
     4801 acgctgtgcg cccagcgcgc gccagtacgc gtcgcgcacc tcctggacaa tattctgcaa
     4861 taccttgcgg cgacgctctt cggcgatgtt gacgttatcc gcctgctgct tcgcgcggta
     4921 gtagctcatg ccgaaatcga gcacgtccca ggagaaggtg gcgtcggcca gcaggtgctc
     4981 gcgctcttca gaggtggacg gacgcaggct gacgacccgg tcttcgatac cgacgctggt
     5041 gccgccggaa tcgttgtcgc gccagcggta gccggcatcg gtcatcaact tcggcagcat
     5101 atcctgctgg gagatatcca ggatgccgcg cgacagggcg gtttccatca tcttcaggcg
     5161 gtagtcgagg ttgtatttca gcgcgcgggc catggcgtct gacagggtca gcggaccaga
     5221 gaccggctgc tggtcgcggt acatggcggc gcggtcggcg ctgacgcgcg cgccgatttc
     5281 atcctgggtc agcggttcag gtttaatggt gccgcaaccg cttaacagca gcgcggaaac
     5341 cagtgcggcg acaggggcga gcagaggcgc agaacgtacg gcagtcgggg ttacggcagt
     5401 taaagtcgtc atcattgtta tgcttaaagt gttgagttaa caatccggcg gcgcggcgcc
     5461 gccggtgtaa aaggtttttg gagttgcgcg attaacgatg cggtgtggcg cctcccacgg
     5521 tgaccggttc cggcgcggcg acgcggttac gggccagcgc gtcgacctgc gccgccagtg
     5581 acggcgcgcc tggtcgggcg gcggcttgcg gcacgatcac cacgcccggc gcggcggcgt
     5641 gaacctgctt cgcatcaggg gttttctcgc cgcgctcctg ctgcacggtg gttctttcgt
     5701 tgatgcgctc gttaagcggc ggacgttccg gcgcgttttc cagcacgggc gcaggctgta
     5761 cggatttatt cagcgagaag tggctgaagt gatcgcccag cagctgcgaa ccgataccgg
     5821 tggcggcgaa ggtgttcacc acgctgcgtt gcagcgtggt gtttacccag cgggtaaaga
     5881 gaatgccgtc ttccagcacc ggcgtgccgt tgtcattcat gccgcggccc agctcggtaa
     5941 ttttgcggtt cagctccacc ttgcgagaga actggtcgct gtacaagccg tagattgcgg
     6001 gcaggataaa cagacccggc gaatacggct gatcgagcga cagctcgtag ttggtgtagt
     6061 agctcgggaa gtgctgatcg cctgtgcgta ccgactgcgg cggcgtcacg ccgtgaataa
     6121 cgatggtcag ctgcgcctgg ctggtattgc cgtcggcgtc ggtaatggtg taagtgaaca
     6181 cctcgctcag ggtggcgttg gcgtccagcg acgccacgcg gctgttgctg cggttcagca
     6241 catagctgta gttgccgcgg gcgtcgagcg tcagggcgcc gaactcgccc aggcttacgc
     6301 cgccgaccgc gcccgcgcgg ttgaggttca cggaggaaat cgcgctcacc gggccgcccg
     6361 ccgcggcgcc gtctgcgccg atagcgtcgt tgctaaagac gttgcccgac gccgcctgct
     6421 ggccgcgatc ctggtcgatg ctgttgtcgt ccgctgccgc gaccggcgcg tcgttgagaa
     6481 tattgacgcg cagcaggccc ggcgcgcggg tctgctggcc gttgacggag aaccgaatct
     6541 cttcgcgggt ggtggattcg cccggctggt taaccgcctg ggtcagcgtg aagcggtatt
     6601 gcagcgtcgc gcggccttcg ccgtccacct ggaaaccggt gagcgacagc acgccgtccg
     6661 gcgttgcgat atccactggc gcctgggcgc tcagcgcctg gagctgcgca agcgtcaggg
     6721 tctggccgcc aatggtcagg ctttccaccg gctccagcga cagtacggcc atcgcgcccg
     6781 tggtggtttg cgcgccgctg gccgtatcga ccagacctgc ctctttcacc cacgcctcgg
     6841 ctacggggtt caccagcagg cgcagttcgc ccgtggcggt gccgccgtcg ttgccgtcgc
     6901 tcagggtgta gttcaacact ggcgtaaagc cggaccagcc gcttgccggg gtaaacagat
     6961 agctgccgtc gcggttaacc accagcgtgc cctggcccgg gatcgtaatg atgtcccccg
     7021 gcgcgtaggt cgcgccgccg acggtgatct ccgccacgtg cagcgggtcg ctgtccgcgt
     7081 cgcggtcgtt ggtcagcaga ttgccgctga ccggcgtttc cggcagggtg ctcgctttat
     7141 ccgccaccgc gaccggcggg gtgttggtga cctcaagcgt gacctgggcg gtttcggtga
     7201 cgccgccgct ggtgacggta taggtgaagg tgtcctggcc gacgtagccc gcgtttggcg
     7261 tataaaccag ctgcccgttt tcaatagcga cggagccgtg cgcgccctgg gtcacgccgg
     7321 taatggcctg gtccgggtta acgaagcggt cgttgccgag cgcgtcaacg gtgaccgggt
     7381 cgcctgcatg ggtggtggcg cggtcatctt tcacatccac gaccggcgta acggtgataa
     7441 cgagcaacgc ggtagcggtg ccgccgtcgt tgccgtcgct gaccgtatag gtcaccaccg
     7501 gcgccgtgcc gttccagtcg gcgactggcg tgaagcggta gctgccgtcg cggttaacgg
     7561 tcagcgcgcc gacgcccgcc agctggacgg tgtcgcccgg ctgagcagtc tggccgccga
     7621 cggtaaagcc cgcaatgtgc agcgggtcgc cgtccgggtc gcggtcgttg gtcagcagtt
     7681 cgccgccaat agcggtgtct tccggcgtgg tgacgatttc accgtcggcc accggcacgg
     7741 tattggtcat cacgacgctc acgg
//
LOCUS       DAOFQD010000182         7582 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.182, whole genome shotgun sequence.
ACCESSION   DAOFQD010000182 DAOFQD010000000
VERSION     DAOFQD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.182"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..770
                     /locus_tag="QBF46_004389"
     CDS             <1..770
                     /locus_tag="QBF46_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HDQ6676493.1"
                     /translation="LPTDNARVADEFFSRFVELQDLLSGGLSEKDEDGLIGTSFSPSS
                     IFYLIDQLSNLRTKSVQLSQLGPFYQYIPNVDLILCTDMDTEPADFVLSSKDKLIYVH
                     IKCGAAGKPESSAGSICEVGSQAIKNIHYLISNDKNLQFANLTRLRNPWPKLGGNKHN
                     IELDSRIRLFEGTFNINHDINDVLEKINKRRASSLVRKEIWIVVGNGFSLSHFKSQFN
                     PSVVKKSQESMQSYQLIDSWLLQSKSLGIDLKFFVSP"
     gene            1088..1762
                     /locus_tag="QBF46_004390"
     CDS             1088..1762
                     /locus_tag="QBF46_004390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676494.1"
                     /translation="MTLNEKPDSSMKIEKTKTLPPAEGERRAMRGYMGQYERAGAAIY
                     AELERGQLEWIGVADRSAGIADDLVLGFNGLIVGHQFKTSRFPGTFTVQTLLVGADGL
                     LKPLVCAWQNLCSANPTSHVEIRLVVNDYPSVNDAPGMEAPAHSAAFLDEFEHFPKRT
                     LEEWRYSNWGGLVEILFQHSCLGGDDFERFFHALRIIHGSAADFIQFHKLSAEQARLA
                     SDIAKI"
     gene            1952..7150
                     /locus_tag="QBF46_004391"
     CDS             1952..7150
                     /locus_tag="QBF46_004391"
                     /inference="COORDINATES: protein motif:HMM:NF024793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ6676495.1"
                     /translation="MALIGPPGSGKSTLLQTTLATEYNTRVVRYLAFIPGAAQGVGRG
                     EADDFFEDISAQLRSSGLPGLRLRDSSQFERREQFGELLKQAGERYQRDTVRTIIIVD
                     GLDHIPREELPAHSLLGELPLPAAIPLGVTFILGTQRLELRHLKPAVQEQAGHPDRLV
                     TMHPLERVAVARMADVLGLDSTISRVKLYELSHGHPLAANYLIKALLSADEQDTSCIL
                     AGGMEFNGDIESVYASAWREIANDPDVMHVLGFIARIEAPMPLKLLATIVDAQAIERT
                     LKTVRHLLKETSKGWTVFHNSFRLFVLSKPKITLGSIDETYSQHIYRELAELSRHAPE
                     HSLQSWLTLRYLARSGERDELLVLATPAYFRHQFAHGRSCSEIDSDIHLALIAARSTY
                     DGVIATRLLLCREEISRRTQALEYANELPRAMLKVGDIDAAISFVQDFPNAGYEVVDL
                     LLEQGDFDRAKELFEHLEPLSHLHTPRFEHYGDSHNLQEFKKWAKRVFHFRDSEQIKQ
                     AIDYLTVEGLKHATSVSTDENITSIREQLKWTVVEAIVNWQADVNIQDTCNQYGIHEQ
                     EIPVLMTQAGFTARDRGNNILALELFKTAMALSDFNDVSNGGRRSIALFYATSGCTDL
                     ASILFENLFAPAISMGDNELESTKALTLAAMEHAQLCVLLGKSLPDVVTSTHAILRPL
                     QTHASETGRLLGLSIINASCIPSGNIQMVCRMVMRYVMQLNSHSGNDTYQAQLALTAT
                     SPLICTLIKISALRGKVEYNSVLNEIDNVMPALILKGNTLLRREIALAMYQADGDRER
                     AAARLEHMVNELVENTPSEQLETLSVLANSFAAIGDVDRALNLLASIHDHCLGYALAA
                     RKDPLYSVWKDILILANAADPEHRAQRIGQLIRQVDGMKETEGASAAYRLTEVLINEA
                     MRMNAHSGYTVAQKLSNWGLIPWPNQVNELVIGMLERRPEMVFLCTPIWCGLCLPFYI
                     EPYYRDPTHVGNYIDVAANAAGPSSIAKLVSILLPAIQVHSRAHERLTLLNRLSKAAL
                     RHGYTDNQLDNAITRWTSEAPEARRSYTPQKYDEASTLDELQQAFESNDSEPEYHAPY
                     RFCELAKSAALDKVVKMYERWHCLQSDARCRFLVAERLVNAGDTTLARKLVDDYDTSS
                     DREMSWSQWLGGNRFRLFRARKLLDGAAIHHEAYEDFISSIVAGKESTMSLLTDMADI
                     LPVICESPDWPAVWSILAEQMSFTREHRIGEIFEFENENITDEELLAELLHFSLRLPI
                     TEARQHAEKTALILAEHSTGGQIVFEHTITRLLTGPLDEPLQALQVLLLLKQNHFAAE
                     IGDLVSGLTNHRDIAVAEAACLLAQYWQLPISIDFHPLPLTYRLALDGDPDHEHALLD
                     PVSGAMRIEVDLGWTQMLRPVARRLAELADCEEMNIRQRAATFIQQWGGLAAFGPGAT
                     KKTESQLRTLSMQISYLKPHAYIGILALRHVAGELSLAGLLSPRDKPSLLEQLDAALP
                     PAPRPEMQIRPTGIRRPLKVKDAPWSEAEEMWTNLVEEDVKPWRDRADEFVIAEVSQF
                     KMHDTRRAEYQVYRISAPQIHISDAKFMAWYQSLPAVVWLGQMIPLDEDLAPTIVRRV
                     VSSIGTMSSPGYAIALCPNIQMHLGWHECSEMPNIYTDQNSKVVARLVNWRDAGPVDI
                     DDDYIWGEGCYLTLSKAGLIQVQTLFGELTVRNFASRSVRQLRQGEAQMIKTAQNLFS
                     TESPRV"
     gene            7195..7443
                     /locus_tag="QBF46_004392"
                     /pseudo
     CDS             7195..7443
                     /locus_tag="QBF46_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7453..>7582)
                     /locus_tag="QBF46_004393"
     CDS             complement(7453..>7582)
                     /locus_tag="QBF46_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDQ6676496.1"
                     /translation="CLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT         2135 a         1684 c         1777 g         1986 t
ORIGIN      
        1 ctctccccac agacaatgca agagttgcag atgaattttt ctcgagattt gtagaattac
       61 aagatttgtt gtcaggtgga ttatcagaaa aagatgagga tggattgatc ggaactagtt
      121 ttagcccctc atcgatattt tatttgatag atcaactgtc aaacctgaga actaaatctg
      181 tgcaactatc tcaattggga ccattttatc aatatatccc taatgttgat ttgattcttt
      241 gtacggatat ggatacagaa ccagctgatt ttgttctttc ttccaaagac aaacttatct
      301 atgtacacat aaaatgcggt gccgcaggaa aacctgaatc ttcagcaggt tcgatttgcg
      361 aagtcggtag ccaagcgata aaaaacatac attacttgat tagtaatgat aaaaatttac
      421 aatttgccaa tttaactagg ctgagaaatc cttggccaaa attaggaggt aataagcata
      481 atatagaatt agacagtaga ataagattgt ttgagggaac tttcaatatt aatcacgaca
      541 taaatgatgt cttagaaaag ataaacaaac gaagagccag ttcattagta agaaaagaaa
      601 tatggatcgt tgttggtaac ggattttcat tgagtcattt taagtcacaa tttaatcctt
      661 cagtagtgaa aaaatcacaa gaatcaatgc aatcatatca acttatagac tcatggctat
      721 tacaatccaa aagtcttggt atagatctta aattttttgt ttctccataa gaaatttcgc
      781 gacagagatc cttaacggtg cgtcgagctt cgacggaatt cagaataatg atggtctggt
      841 gttcggtgaa tcgtgctttg cgcatggcaa tctcctatca gaacaaaacc agtatgccgg
      901 atgatctcta aaagtgaatg gaccgatatg cagggatgct tacagtgggt cttcgacctt
      961 tataagcata gtaaagaata gaatatgcca atgtacgata atctgtgcac tctattacct
     1021 gcgcaaaaaa gtacaccaga attgtttgtc tggtttggca aatcgagatc attaggcggc
     1081 atagcgaatg actttaaatg aaaagcccga ttcatcaatg aagattgaga aaacaaaaac
     1141 cttgccccca gcagagggcg agcgccgggc aatgcgtggc tatatgggcc aatatgaaag
     1201 agccggtgca gccatttatg ctgaattaga gcgtgggcaa ttggagtgga taggcgtggc
     1261 ggaccgcagt gcgggtatcg ctgatgattt agtacttgga tttaatggcc ttatcgttgg
     1321 gcaccagttc aaaacgtccc gtttcccagg tacatttaca gtacagacac tcttagtagg
     1381 ggctgatggt ctgcttaagc cattagtttg cgcctggcaa aatctttgta gtgctaaccc
     1441 aacgtctcac gtagaaattc gtttagttgt caacgactat ccatcagtaa acgacgcccc
     1501 cggaatggaa gctccagctc acagtgctgc cttccttgat gagtttgaac attttcccaa
     1561 acgcacgctt gaggaatggc gctacagtaa ctggggcggt ttagtcgaaa tattgtttca
     1621 acattcttgc ctaggtggcg atgatttcga gagatttttt catgcgttgc gcataattca
     1681 tggctcagca gcagatttta tacaatttca taaactcagt gcagaacaag cgagactggc
     1741 gtctgatata gcaaaaatat aacctcagtt ggtctccgat aaacgagata gggaccgatg
     1801 gtcctgtgaa gaactattaa atgaactagg gtggaaagat ccgaccaaaa cacgccacgt
     1861 acatcgcttt cccatcggtg ctcacgtcca acgcaaccgc gatacggaac tacaacttct
     1921 ccagacgata cgcaacacaa tccagggcta tgtggcattg attgggcctc caggttcggg
     1981 gaaatcgacc ttgctacaga caaccctggc taccgaatat aacactcggg tcgtgcgcta
     2041 tctggctttc ataccgggag ctgcgcaagg tgtaggacgc ggggaagctg atgatttctt
     2101 cgaagacatt tctgcccagt tacgcagcag cgggctgcct gggcttcgcc ttcgagacag
     2161 cagccaattt gaaaggcgcg aacaattcgg tgaactgctc aaacaagctg gcgagcgtta
     2221 tcaacgtgat acagtaagaa ccatcattat tgttgatggg ctggatcata tcccccgcga
     2281 agaactacca gcccattcgc tgttaggcga attgccgctg cctgcagcca tccctttggg
     2341 cgtgacattt atacttggta cccagcgact ggaactcagg catctcaaac ccgcagtaca
     2401 ggaacaggct gggcatccgg atcgtctcgt aacaatgcat ccacttgaga gagtggcggt
     2461 cgccaggatg gcagacgttt tgggtcttga ttcaaccatt tcgcgtgtaa aactttatga
     2521 acttagccac ggtcatccgc tggcggccaa ttatctcatt aaggcactgt tatcggcaga
     2581 tgaacaggac acatcatgca tcctcgccgg agggatggaa tttaatggcg atattgaatc
     2641 agtttacgca tccgcctgga gagaaatcgc aaacgaccct gatgttatgc atgttctggg
     2701 tttcattgcc cgtatcgaag ctccgatgcc gctgaaattg ctggcaacaa tcgtagatgc
     2761 tcaggcgata gagcgtacct taaagaccgt ccggcattta ctcaaggaaa cctcaaaggg
     2821 gtggactgta ttccataaca gcttccgtct atttgtgctc tccaaaccaa agataacact
     2881 gggcagtata gatgaaacct attcacaaca tatttatcgt gaattagctg aactatctcg
     2941 tcatgcacca gagcattcat tacagtcctg gctaacactg cgctatctcg cccggtcagg
     3001 agagcgtgat gaacttctgg tgctcgcaac gccagcatat tttcgacacc agtttgcaca
     3061 tggacgttcc tgttcagaga ttgattcgga catccacttg gctctgattg ctgcacgttc
     3121 cacgtatgat ggtgtaattg ccacacggtt gctactttgc cgtgaagaga tatccagacg
     3181 aactcaagca ctggagtatg ctaatgaact tccgcgcgcg atgttaaaag ttggcgatat
     3241 tgatgcggcg atctctttcg tccaggactt tcccaatgcg ggctatgaag ttgttgacct
     3301 gcttttggaa cagggtgatt ttgaccgcgc gaaagaactg tttgagcacc ttgagccatt
     3361 gtctcacttg catactccca gattcgagca ctatggggac tcgcataatc tacaagaatt
     3421 caaaaaatgg gcaaaacgag tttttcactt ccgcgactct gagcaaatta agcaggcaat
     3481 agactatttg accgttgagg ggttaaaaca cgccacaagt gtatcaacag atgaaaatat
     3541 tacctctatt cgcgaacagt taaagtggac agtggtcgag gcaattgtta actggcaagc
     3601 agacgttaat attcaggata cctgcaatca gtatggcatt catgagcaag agataccggt
     3661 tttgatgact caggctggat ttactgctag agacagagga aataatatct tagcattgga
     3721 attatttaag actgccatgg cattgtctga ttttaatgat gtttctaatg gcgggcgaag
     3781 atcgattgca ttattttatg ccacctcggg ttgcaccgat ctggcttcaa tattattcga
     3841 aaaccttttt gcacctgcaa tttcgatggg agacaatgaa ttagaatcaa caaaagcact
     3901 gactcttgca gccatggaac atgcgcaact ttgcgttttg ctcggcaaat ccctgcccga
     3961 cgtagtcacc tccacacacg ctatcttacg accgctgcag acacatgctt cagaaacggg
     4021 acgcttgttg gggctgtcca taataaatgc atcatgcatt ccttctggaa atattcaaat
     4081 ggtctgtcgc atggtgatga gatatgtaat gcaactcaat agccattctg gaaacgatac
     4141 ctatcaggct caattggcat tgacagctac atcaccactg atttgtacat taattaaaat
     4201 ttctgcgctg cgtggtaagg tcgaatataa ttcagtacta aatgaaattg ataatgtaat
     4261 gcctgcttta atattaaaag gcaatacact actccggcgt gaaatagcat tggcaatgta
     4321 tcaggctgac ggtgaccgtg aaagggcggc cgccagactt gagcatatgg taaacgagtt
     4381 ggtagaaaat acacctagcg aacaactcga gaccctgtca gttctggcaa acagctttgc
     4441 tgcaattggt gatgttgacc gggcactaaa cttacttgct tcgatacatg accactgttt
     4501 aggctacgct ctggcagcgc gtaaggaccc tttatactct gtctggaaag acatattgat
     4561 tttggccaat gcggcagacc cagaacaccg tgctcaacga ataggtcagt tgatacgaca
     4621 ggttgatggt atgaaggaaa ccgagggagc atctgccgca tatcgtttga cagaagtgtt
     4681 aatcaatgaa gcaatgcgta tgaatgcgca cagtggttat accgtggcac agaaactcag
     4741 caactggggg ctgattccat ggccaaatca ggtaaatgaa ctggtaattg gtatgctaga
     4801 acgccgtcct gaaatggtgt ttctctgtac accaatttgg tgcgggctat gccttccatt
     4861 ctacattgaa ccctattatc gtgaccctac acatgtaggc aattatattg acgttgctgc
     4921 aaatgcagcg gggccttcat cgattgccaa actggtatca attctattac cggcaatcca
     4981 ggttcatagt cgagctcacg agcgactcac actattaaat cgcctgagca aggcggcatt
     5041 gagacacggt tataccgata accaacttga taatgccatt actcgatgga cttcagaggc
     5101 ccccgaagcc cgccgctcct acacgccaca aaagtacgac gaggcttcaa cccttgacga
     5161 acttcaacag gcatttgaat caaatgattc agaacctgag tatcatgcgc cttatcgttt
     5221 ttgtgagctt gcaaagtccg ccgcattaga caaggtggtg aaaatgtatg agcgctggca
     5281 ttgcctgcag tcggatgcac gttgtcgttt tttggttgca gagcggctag ttaatgcggg
     5341 ggacactacg ttagccagaa aattagttga tgattacgat accagtagtg accgggagat
     5401 gtcatggagc caatggttag gaggaaatcg attccgtctc ttccgcgcgc gtaagctact
     5461 cgatggcgca gcaattcatc atgaagcata tgaagacttc atcagttcaa ttgtggcggg
     5521 gaaagaaagc accatgtcgt tgctaacaga tatggccgac attcttcctg tgatctgtga
     5581 gtcgccagac tggcccgccg tttggtctat cttagcagag cagatgtctt tcactcgcga
     5641 acaccgtatt ggtgaaattt tcgaatttga gaatgaaaat ataaccgacg aagagttact
     5701 cgcggaattg ctccattttt cattacgatt gcctatcacc gaagctcgac aacatgcaga
     5761 gaaaactgca ctaattctgg cggaacattc aacaggaggg caaatcgtat ttgagcacac
     5821 tatcacacga ctcctgactg gcccccttga tgaaccactc caggcattgc aagttttgct
     5881 tttgctaaaa cagaaccact ttgcagctga aattggtgat ttagtctctg gccttacgaa
     5941 tcatcgtgat atagctgttg ctgaagctgc gtgcttgcta gcacaatatt ggcagctacc
     6001 tatatcgatt gattttcatc cgttgccgtt gacatatcga ttggcactcg acggagaccc
     6061 tgatcatgaa catgctctgt tagatcctgt gagtggggcg atgcgtattg aagtcgactt
     6121 aggatggacg caaatgcttc gtcccgttgc aaggagactt gcagagctgg ctgattgtga
     6181 ggaaatgaac atacgccagc gtgccgcaac gtttatccag caatggggag ggctggcagc
     6241 ctttggccct ggagcaacaa aaaagaccga atctcagtta cgcacactct caatgcaaat
     6301 ctcctatctt aagccccatg cttacattgg catactggca cttcgtcatg tcgctggaga
     6361 gctgagcttg gcaggcttgc tctcaccaag ggataaacca tcgctactgg aacaattgga
     6421 tgcagcactt ccgccagcac ctcgccctga aatgcaaatc cgaccaactg gcattaggcg
     6481 accgctgaaa gtcaaggatg ccccgtggag tgaagctgaa gaaatgtgga caaatttggt
     6541 tgaagaggat gtaaaaccct ggagagatcg tgccgatgaa ttcgtaatag ccgaggtttc
     6601 acaattcaaa atgcatgata cccggcgagc tgaataccag gtctatcgaa ttagcgcacc
     6661 tcaaattcat atttctgatg ccaaattcat ggcatggtat caaagtttgc ccgcagtcgt
     6721 ttggctgggt caaatgatcc cactcgacga agacctcgca ccgacaatag tcagacgtgt
     6781 agtcagttcc atcgggacaa tgtcttcgcc gggatatgcc attgcattat gccctaatat
     6841 ccagatgcat ctgggatggc atgaatgcag cgagatgcct aatatttata ccgaccagaa
     6901 ttcaaaagtc gtagcaagat tagtgaattg gcgagacgcc ggaccagttg atattgatga
     6961 tgattatata tggggggaag gttgctatct gacgctttcc aaagcaggct tgatacaagt
     7021 ccagactctg tttggtgagc tcaccgtacg taatttcgca agcagatctg ttcggcaatt
     7081 acgacaaggc gaagcgcaaa tgataaagac agctcagaat cttttcagta ctgaatcgcc
     7141 acgggtttaa cagacacctc ggagtcattt aagatggctt aaagagaggt gcccatgagc
     7201 ggtaagcgtt atcccgaaga gtttaaaatt gaagcagtca aacaggttgt tgatcgtggt
     7261 tattctgttt ccagcgttgc aacacgtctc gatatcacca cccacagcct ttacgcctgg
     7321 ataaagaaat acggtccgga ttcttccaat aataaagaac agtcagatgc tcaggccgag
     7381 attcgccgtc tccagaaaga gctgaagcgg gttacagacg aacgggatat attaaaaaaa
     7441 gccgctgtag attcaatctg tcaatgcaac acccctttca attatctctt tcggtgtttt
     7501 gaacttcagt gtctttctcg gtctgttgtt tagctgagca gcaaccagat ctagttcatg
     7561 ttgagtatat tgggcaagac at
//
LOCUS       DAOFQD010000183         7581 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.183, whole genome shotgun sequence.
ACCESSION   DAOFQD010000183 DAOFQD010000000
VERSION     DAOFQD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.183"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1137)
                     /gene="pdeC"
                     /locus_tag="QBF46_004394"
     CDS             complement(<1..1137)
                     /gene="pdeC"
                     /locus_tag="QBF46_004394"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDQ6676497.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAENEGMIEQI
                     TDYVIDNVFRDLGACLATHADRYVSINLSASDFHTSRLIARINQK"
     gene            1567..1848
                     /locus_tag="QBF46_004395"
     CDS             1567..1848
                     /locus_tag="QBF46_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDQ6676498.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(1947..2483)
                     /gene="ssb1"
                     /locus_tag="QBF46_004396"
     CDS             complement(1947..2483)
                     /gene="ssb1"
                     /locus_tag="QBF46_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDQ6676499.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            2737..5559
                     /gene="uvrA"
                     /locus_tag="QBF46_004397"
     CDS             2737..5559
                     /gene="uvrA"
                     /locus_tag="QBF46_004397"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDQ6676500.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(5594..5950)
                     /locus_tag="QBF46_004398"
     CDS             complement(5594..5950)
                     /locus_tag="QBF46_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDQ6676501.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(5954..6370)
                     /locus_tag="QBF46_004399"
     CDS             complement(5954..6370)
                     /locus_tag="QBF46_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDQ6676502.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(6481..7194)
                     /gene="aphA"
                     /locus_tag="QBF46_004400"
     CDS             complement(6481..7194)
                     /gene="aphA"
                     /locus_tag="QBF46_004400"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDQ6676503.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1903 a         1896 c         1894 g         1888 t
ORIGIN      
        1 tttctgattg attcgcgcta tcaaccgtga cgtatgaaaa tcggaggccg acaggttaat
       61 agaaacatag cgatcggcat gtgttgccag gcacgcgccc agatcgcgga agacgttatc
      121 aataacataa tcagttatct gttctatcat cccctcattt tctgccagcg gaataaactc
      181 tgccggattc attatttgcc cctgctcacc aggccaacgt aacaacgctt cagcgccgat
      241 acatttttct gttttgatat cgattattgg ctggtaataa agacaaagtt gatgtttttc
      301 gagggcgcgt tgcaatttac gtttgggaga taaatagttt tgtcgaatac gtagccagag
      361 taatagcaga acaagactcc ccaaaatacc ggcgggcaac gcaaaaataa gatgattata
      421 aaaatgggtt ataagacgtt gatatgaagt cgcaacaatg gctgcaattg ggcgttttgt
      481 cgaataaact gtcgcatata aatagccatt tctttgtacg gttaaatcct tcaaatgaat
      541 cagcggagaa aacgttgctg ccgaggcctc tttgcttaac gagaaaaagg ttttcgtcac
      601 cgtatcatac acaccccatt gcaatgtcgg atcatcagac atcacttcac tccagaagag
      661 agggttgata accgccacat aatttccccg ctgcatatag gtcattttat agccagagaa
      721 aaaaggcgta tcgcggtaat aatagataga aacgttaggt tcacgcttat aatcggccgg
      781 tgcaatcgta tagccgttta caggcgctat cagcgatgag cataaaaaat ggttatcacg
      841 ggcatagatc aattcattaa tataaagata gccacgaata atattcaaca ttcgcttttg
      901 atgggctgga gtgcaaactt gcccctgata gcgttcagct gcatcgctca ctaaatctgc
      961 ctggcgaatg accagctcgg atttatccag tgccagttga gcaaaggttc ggagctgatt
     1021 attcacttct gattttgccc aaaggaaggc aatccatagc gaaagaatga tgggaaagag
     1081 aactaaaaag ataatgcccg gcaacgccag taattggtgt cgtgcacgat gactcattgc
     1141 tatcaatttc ctgcctgttt atacccggcc attgcaaggt gaaaaccctg ggtaccgacg
     1201 tctttagata aatcttacgg tataaatagc acttttgcct gatttgaaat tgataccttt
     1261 ctaatcgtca cctggatgaa aataattcat tgatattcaa aattaatttc ctacaggaat
     1321 gcagaggcgg cgggaagata tgcccttaat gccactgaaa aaccacataa tctgtacagc
     1381 aataaaagtc acggcctaat atggctaaca aacaatcaat tgtgatatag ttcacaaaat
     1441 taatgaaaca aacagagtgt ttcatttttg tgttctgcaa gccccgcgat agccagagtg
     1501 taccacgcct cccgtgaaca acgccgcgct gtccagggca tcggctcttt tcagaggaat
     1561 tgtttgatgg ctaccctcac cactggcgtg gttcttcttc gctggcaact tcttagtgcc
     1621 gtaatgatgt ttctggccag cacactcaac atccgttttc gtcgatctga ttatgtcggg
     1681 cttgcggtga tcagcagcgg tctgggcgtt gtttccgcct gttggttcgc aatggggttg
     1741 cttggcatca caatggcgga catcaccgct atctggcata acatcgagtc ggtgatgata
     1801 gaagagatga atcagacacc gccacaatgg ccaatgattt tgacctgata cgtagaagcc
     1861 tccataacgg aggcttcttt tttatggctg gcagatgctt taatcatcca ccttaaaaca
     1921 atataaccta ttgttttaat gacaaatcag aacggaatgt catcatcaaa gtccatcggc
     1981 ggctcgttag acggcgctgc cggagcggac tgctgcgggc gagactgcgc gccgccgctg
     2041 aactgattgc caccctgcgg ctgctgaggc tgaccccaac cgccctgcgg ctgaccacca
     2101 ccgatattac cacctgccgg agcgccacca ccctgacgac cacccagcat ctgcatggtg
     2161 ccgccaacgt tcaccacgac ttctgtggtg tagcgatcct gaccggattg atcggtccat
     2221 ttacgggtac gcagctgacc ttcgatataa acctgagaac ctttacgcag atattcgctg
     2281 gccacttctg ccagtttgcc gaacagcaca acgcggtgcc attcagtctg ttctttcatc
     2341 tcgccggtcg ctttatcacg ccaggattcg gaagtagcca gcgtaatgtt ggcaactgcg
     2401 ccaccatttg gcatgtagcg tacttccggg tcctgaccca gattaccaac gagaataacc
     2461 ttgtttacgc ctctgctggc catgttcgtg tctcctgaaa aaaatcgttc tgaataagtg
     2521 taaacgcgcg attgtaccat taccaatagc gcttttacta tgttgtgacc tcggttccgg
     2581 gaaacaaacc tggccagaca ttgttacaca acactccgga taatgcattc caatactgta
     2641 tattcattca ggtcaatttg tgtcataatt aaccgtttgt gatcgccggt agcaccatgc
     2701 caccgggcaa aaaagcgttt aatccgggaa aggtgaatgg ataagatcga agttcggggc
     2761 gcccgcaccc ataatctcaa aaacatcaac ctcgttatcc cccgcgacaa gctcattgtc
     2821 gtgaccgggc tttcgggttc tggcaaatcc tcgctcgctt tcgacacctt atatgccgaa
     2881 gggcagcgcc gttacgttga atccctttcc gcctacgcgc ggcagtttct gtcactgatg
     2941 gaaaagccgg acgtcgatca tattgagggg ctttctcctg ccatctcaat tgagcagaaa
     3001 tcgacgtctc ataacccgcg ttctacggtg gggacaatca ccgaaatcca cgactatttg
     3061 cgtttgttgt tcgcccgcgt cggcgaaccg cgctgtccgg accacgacgt cccgctggcg
     3121 gcgcaaaccg tcagccagat ggtggataac gtgctgtcgc agccggaagg caagcgtctg
     3181 atgctgctcg cgccaatcat taaagagcgc aaaggcgaac acaccaaaac gctggagaac
     3241 ctggcaagcc agggttacat ccgtgctcgt attgatggcg aagtctgcga tctttccgat
     3301 ccgccgaaac tggaactgca aaagaaacat accattgaag tggtggttga tcgcttcaag
     3361 gtgcgtgacg atcttaccca acgtctggcc gagtcgtttg aaaccgcgct agagctttcc
     3421 ggtggtaccg cggtagtggc ggatatggac gacccgaaag cggaagagct gctgttctcc
     3481 gctaatttcg cctgcccaat ttgcggctac agtatgcgtg aactggagcc gcgactgttt
     3541 tcgtttaaca acccggcagg tgcctgcccg acctgtgacg gcctcggcgt acagcaatat
     3601 ttcgatcctg accgcgtgat ccaaaacccg gaactgtcgc tggcaggtgg tgcgatccgt
     3661 ggctgggatc gccgcaactt ctattatttt cagatgctga aatcgctggc agatcactat
     3721 aagttcgacg tcgaagcgcc ctggggcagc ctgagcgcga acgtgcataa agtggtgttg
     3781 tacggttctg gcaaagaaaa cattgaattc aaatacatga acgatcgtgg cgatacctcc
     3841 attcgtcgtc atccgtttga aggcgtgctg cacaatatgg agcgccgtta taaagagacg
     3901 gagtccagcg cggtacgtga agaattagcc aagtttatca gcaatcgccc atgcgccagc
     3961 tgcgaaggaa cgcgtctgcg tcgggaagcg cgccacgtgt atgtcgagaa tacgccgctg
     4021 cccgccatct ccgacatgag catcggtcat gcgatggaat tcttcaacaa tctcaaactc
     4081 gcaggtcagc gggcgaagat tgcggaaaaa attcttaaag agatcggcga tcgtttgaaa
     4141 ttcctcgtta acgtcggcct gaattacctg acgctttccc gctcggcaga aacgctttcc
     4201 ggcggtgaag cccagcgtat ccgtctggcg agccagattg gtgcgggcct ggttggcgtt
     4261 atgtacgtgc tggacgagcc atctatcggc ctgcaccagc gcgataacga gcgcctgttg
     4321 ggtacgctta tccatctgcg cgatctcggt aataccgtga ttgtggtgga gcatgacgaa
     4381 gacgcaattc gcgccgctga ccatgtgatc gatatcggtc ctggtgcggg tgttcacggc
     4441 ggtgaagtgg tcgcagaagg tccgctggaa gcgattatgg cggtgcctga atcgttgacc
     4501 gggcagtaca tgagcggtaa acgcaagatt gaagtgccga agaaacgcgt tccggcgaat
     4561 ccggaaaaag tgctgaagct gacaggtgca cgcggcaaca acctgaagga cgtgacgctc
     4621 acgctgccag tcggtctgtt tacctgcatc acaggggttt caggttccgg taaatcgacg
     4681 ctgattaacg acacactgtt cccgattgcc caacgccagt tgaatggtgc gaccatcgcc
     4741 gaaccggcac cgtatcgcga tattcagggg ctggagcatt tcgataaagt gatcgatatc
     4801 gaccaaagcc caattggtcg tactccacgt tctaacccgg cgacctatac cggcgtgttt
     4861 acacctgtgc gcgaactgtt tgcgggcgta ccggaatccc gtgcgcgtgg ttatacgcca
     4921 ggacgtttca gctttaacgt ccgtggcggg cgctgcgaag cctgtcaggg cgacggtgtg
     4981 atcaaagtgg agatgcactt cctgccggat atctacgtgc cgtgcgatca gtgcaaaggt
     5041 aaacgctata accgtgaaac gctggaaatt aagtacaaag gcaaaaccat ccacgaagtg
     5101 ctggatatga ccatcgaaga ggcgcgtgag ttctttgatg ccgtacctgc actggcgcgt
     5161 aagctgcaaa cgttgatgga cgttggcctg acgtacattc gactggggca gtccgcaacc
     5221 accctttcag gcggtgaagc ccagcgcgtg aagctggcgc gtgaactgtc aaaacgcggc
     5281 accgggcaga cgctgtatat tctcgacgag ccgaccaccg gtctgcactt cgccgatatt
     5341 cagcaactgc tcgacgtact gcataaactg cgcgatcagg gcaacaccat tgtggtgatt
     5401 gagcacaatc tcgacgtgat caaaaccgct gactggattg tcgacctggg accagaaggc
     5461 ggcagtggtg gcggcgagat cctcgtctcc ggtacgccag aaaccgtcgc ggagtgcgaa
     5521 gcttcgcata cggcacgctt cctcaagccg atgctgtaat cgttaaggcc gctttctgag
     5581 cggccttttc ctttcagagt tgtaccagca atttacgctt ttcttccggt agtaaattca
     5641 ctgcctgctg ataagacgca tccaccagat aatagatttg cgaatccggc agcgaaccgt
     5701 cgagatacac ggtgctccag tgcgctttat tcagatggcg gctaggacgc acatcgctgt
     5761 gttgctgacg tagcagctcc gccagctccg ggctggtttt cagcgaaaca gctgggcgat
     5821 tttctacttc tttcaccatc gcaaacagta catcttccac tttgatctgc gtcgctttcc
     5881 agtcgttatg cacgctctgt tctgcgcctg gttttgccat gcaatattgt agcaactccg
     5941 aaatggtcat tttttactcc ccttgtagtg tcgcgatgat gcgacgcgat ccgccgtgga
     6001 tgcgatgttc ccccagccag atgccttgcc aggtgccggt ctgaatacgc cctttatgta
     6061 acggcaatac aagcgatgtt cccagcattg aggatttgat atgagaaggc atatcgtctg
     6121 ctccctcata gtcatgctca taatttccgt tgtcgggaac ggtgcggagg aaaaaacgct
     6181 ccatgtcgtg gcgtacggtg ggatcgcagt tctcattaag tgtcagagag gcggaggtat
     6241 gttgcagcaa cagatgcagt aagccgatgt taacgcgcgg catatcagcc agctgattca
     6301 gaatttcatc cgttaccaga tgaaacccac gagatttggc actaagcgtg agcgtctttt
     6361 gataccacat gtgctgctcc ttgataaaac tctcttaatc agtttgcagc aagacagcga
     6421 aaggataaag gtgtgattaa aaaaacagca ttcgggagag catcacgctc tcccgctctg
     6481 tcagtattct gaattgacga tcacctcttc accaaacgca cccgcttgtg gcaagggttt
     6541 gtaggtagag ttggaggcgc gcagaatgcg gataccacga gcgccgacat cgcgtgcggc
     6601 ggtaatatcg ttatcggaat cgccataaaa aattcggata tttttatcct gcagccattg
     6661 cgattttgta ttttgccctg gtttatcgcc cgcaaagatt accggattca tgttggtggc
     6721 aggaatatga aaattatccg ccagcgtttt tgaaaccgtt tctgttttcg tcgggctacg
     6781 accagtcaca aagaagatcg cgtcaccgcg gcgtacatgc atatcaatca gctggcgagc
     6841 gacctctttt ggaatgctga attcatccca gccattattc attttttccc agaacacagg
     6901 atttttcaga taatcttcgc tttctggcga gaaggttttt ttgccgcgcc agaaccccgg
     6961 actggaaaaa agtaccgtgt catcgatatc aaaccccacc gccattggcg gacgccctgc
     7021 gaggctattt tcaatttgtg cgaccgaaac ccaatgaatt ggtgcctgtt cagcaagcct
     7081 ggcaacgtta gtcccagggt taagcggtga aggagatgag gccagggcaa cagcggaact
     7141 gtttagagcg aacaataagc aaacggcact gattgcctgt gtgatcttgc gcatattttt
     7201 ccctaaatag tcagtatgtt gaaacttttt attgtgagat ttgttgcaaa aaccatctga
     7261 ccataacgcc agctgccggg gaaaggaagg attatctgcg gtttttgtga tgaaaagcag
     7321 gatgattata tttaacccag taaatacgtt cctgtctttt tttgtataaa aaatgtttat
     7381 tactcgatgt aaggatacat taattttacg tttattatga tgaatcctcg gcaaatgagc
     7441 gaaatttgat agaacattta tctgctgtac gatttaatga acttaatgat acagcattac
     7501 aaaaacaatc gaagtttata aagatgattt ctgattgacc agccccccta ccctacaatg
     7561 gtgattaatt ttattactcg c
//
LOCUS       DAOFQD010000184         7570 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.184, whole genome shotgun sequence.
ACCESSION   DAOFQD010000184 DAOFQD010000000
VERSION     DAOFQD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.184"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="QBF46_004401"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="QBF46_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDQ6676504.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="QBF46_004402"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="QBF46_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDQ6676505.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="QBF46_004403"
     CDS             complement(2659..5772)
                     /locus_tag="QBF46_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDQ6676506.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQVADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="QBF46_004404"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="QBF46_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDQ6676507.1"
                     /translation="MNRRRKLLIPLLFCGAMLIACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="QBF46_004405"
     CDS             7014..7292
                     /locus_tag="QBF46_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676508.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLIS"
     gene            complement(7293..>7570)
                     /gene="gadE"
                     /locus_tag="QBF46_004406"
     CDS             complement(7293..>7570)
                     /gene="gadE"
                     /locus_tag="QBF46_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDQ6676509.1"
                     /translation="PIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLIL
                     KNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF"
BASE COUNT         1952 a         1775 c         1821 g         2022 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca agagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggtttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcaa cattttatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcta cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaataagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcgat
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtagttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatctcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg
//
LOCUS       DAOFQD010000185         7567 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.185, whole genome shotgun sequence.
ACCESSION   DAOFQD010000185 DAOFQD010000000
VERSION     DAOFQD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.185"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            17..235
                     /locus_tag="QBF46_004407"
     CDS             17..235
                     /locus_tag="QBF46_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDQ6676510.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            453..695
                     /locus_tag="QBF46_004408"
     CDS             453..695
                     /locus_tag="QBF46_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676511.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1025..1954)
                     /gene="fdhE"
                     /locus_tag="QBF46_004409"
     CDS             complement(1025..1954)
                     /gene="fdhE"
                     /locus_tag="QBF46_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDQ6676512.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1951..2586)
                     /gene="fdoI"
                     /locus_tag="QBF46_004410"
     CDS             complement(1951..2586)
                     /gene="fdoI"
                     /locus_tag="QBF46_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDQ6676513.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2583..3485)
                     /gene="fdoH"
                     /locus_tag="QBF46_004411"
     CDS             complement(2583..3485)
                     /gene="fdoH"
                     /locus_tag="QBF46_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDQ6676514.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3498..6548)
                     /gene="fdnG"
                     /locus_tag="QBF46_004412"
     CDS             complement(3498..6548)
                     /gene="fdnG"
                     /locus_tag="QBF46_004412"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5961..5963),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDQ6676515.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFTLDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6742..>7567
                     /gene="fdhD"
                     /locus_tag="QBF46_004413"
     CDS             6742..>7567
                     /gene="fdhD"
                     /locus_tag="QBF46_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDQ6676516.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRL"
BASE COUNT         1712 a         1965 c         2051 g         1839 t
ORIGIN      
        1 aggtaaggtg gtggtaatgg cgatgaatac ggtttttctt catttatcag aagaggcaat
       61 taaacggctg aacaagcttc ggggctggcg taaggtttca cgatctgcaa ttttacgcga
      121 agcggtagag caatatctgg aacgtcagca attcccggtg cgtaaagcaa agggaggcag
      181 gcaaaagggg gaggtagttg gtgttgatga tcagtgtaag gagcataagg aatgacaaaa
      241 atttttcatt cttgaatata aaaaacagat gcccttattc tggtattaat acaaggctgt
      301 tttacttgaa cttataataa ctgcaactgt tacatcatat ctggaaaacg cctcgcaaaa
      361 ataaaaaatg atgcgtaaat gagctttctt taaagattcg gcaagggtac ttttctctcc
      421 atagattcaa aaatgatatt ggcgagatat ttatgaactc tctggcaggt atagatatgg
      481 gcagaatttt actcgattta tcgaatgagg tgattaagca acttgatgat ctcgaggtgc
      541 agcgtaatct tcctcgtgcg gacctattaa gggaagcggt agatcaatac ctgataaatc
      601 aatcgcaaac agcaagaacc agtgttcctg gcatctggca agggtgtgag gaagatggtg
      661 tcgaatatca gcgtaagctg cgcgaggaat ggtaatggga aaaaatgatg tcaatcagat
      721 tgctgacaac gtgcgcgttg ttcatgccgg atgcggcgtg aacgccttat ccggcctaca
      781 aagtcgtata aattcaatgt attgcagttt attggtaggc ctgataagcg cagcgcatca
      841 ggcaatctta cgcttgtcat cagtctcatg cccataacct tattgatgtg gtggtgggta
      901 aattgaggcc aaaccggcac tggcggcata gcttgaggtg gtaacgcgga atacgagaga
      961 tggtgtcgtc acaccctatg agctgtaatc cgggcgacct gcgccacccg gaaaacgtca
     1021 gagattactc tccttcaccc ggaaacagga acgggttgat ggaactgcgg gcatagcctt
     1081 cttgctccat tcgtgcgtcc agtaccagag aggcgaggtc atctgctacg gcttcgattt
     1141 ttggatcttt ttcctgatag agaattttca ggtaagtgcc gcagtcatcg cagctttcgg
     1201 ctttaatcgc ggcctgttcg tcatccagcg accagtaatg cagtttgccg ctctgctcgc
     1261 agttgctgca ttttacgcgc actacgtgcc attcagtttc acacaggttg cagtgcaggt
     1321 aacgtagccc ctgagtggtg ccaatttgca ccatgctgga caccggcata gaaccacaaa
     1381 ccgggcaata ttgacgttgc tcgccgtatt cagcgcgggc tttgccgggg atcagattgg
     1441 ccatctgcgc ccagtagagc gacagtgcag cccagataaa cggcgcttta tcgctgctga
     1501 cggacgagaa atcagaggca aacagcgcgc tggccatatc ttccagctcc tgagtcgatg
     1561 ccttctccag attctcaatc actgccagcg ccgggccgct catttcaggt ttcagctcag
     1621 caatcagcgc catcagcagc ttttgccagt gcttatcacg cggcagaacg tgaatatcca
     1681 gcgggggctt gccttgtgcg ctggcttctt taatgcgcgc agtcagatcc atctccagcg
     1741 gatggtcgta cagcaccact tcctgggcgt gggcgataag cgcagcaaag cgcaggtaat
     1801 cacccagagg attattttct gccagctcgc gcagacgctc ggcgcggcgg ttgtataaat
     1861 tcttgagccg agggaacaat aacggcggaa tcatatccgc cgtacgtttc tcgcttgaac
     1921 ccagctcatc ttgcgggatt atgcgaatac tcattcagct ttcttttccg ttgtcttgcg
     1981 gacctcacgg taccagcgcg ggtgatgttt cttcgcccag gcgctggtaa cccatccttc
     2041 caccatggcg gtaatcgtgc ctttcaccca aagggcggcg tagatatgca ccatgataac
     2101 cacaattaac gccactgcgg caaatgaatg cagcattaac gcgaatcgga tcaccgggat
     2161 tgagaaagca ggcgcaaaat aaggacgcca gataatcacg ccgctcacca gcagcagaac
     2221 caggaaaata atcgccgccc agaaaacgca tttctgaccg aagttataac gcccggtgtc
     2281 acctacttcc tcgttgacga cgatcttacg aatattcttc gcccaaaaga tatcatcccg
     2341 attgattagg ttgtgatgcc agtaacggaa aaacatgatg atgaacgagg caaacataac
     2401 cacgccgaca aacgggtgca gaattcgcgc cagctgcggt gtgcccatga tttgcatcaa
     2461 ccagttgaag gacgggaaca aaaagcccag cccgctcacc gccgccagga tgaagcagaa
     2521 ggcggtgatc cagtggttga tacgttccgg cgcggtgtag cgcacgatgg tgtcacgtcg
     2581 tttcatttgc gctcctcgtc tttctcttcg tgcagattat tctcttcctc atccgcacgg
     2641 ttcggaccga cacccacgta gtggaagata ctggccgcaa aggtagccgc aaagccaaca
     2701 gccgcgagcg gtttccagat gcctttccag aatttcacgg tttcgctgat ttccgggttc
     2761 tccggcaagc catgatacag atttggcttg tcagcatggt gcagcacgta catgacgtgt
     2821 gtaccaccga cgcctgccgg atcgtacaga cccgcattgt cgtaaccgcg ggttttcagc
     2881 tcagcaacgc gctcgctcgc cagcgttttc atcgactctt tcgtaccgaa gtgaatcgcg
     2941 cccgttgggc aggtcttcac gcaggctggt tcttgcccaa ccaccacgcg gtcaacgcac
     3001 agcgtacatt tgtagacgcg gttgtcttcc gggttgaggc gcggaatgtc gaacggacag
     3061 cccgcaatgc aataaccgca gccaatgcac tgctcggact ggaagtcgac gataccgttg
     3121 gcatactgaa taattgcacc ttccgccggg cacgctttca ggcagcctgg atcggaacag
     3181 tgcatacagc cgtctttgcg gatcagccat tccagtttgt cgttctgctc cacttccgag
     3241 aagcgcatta ccgtccacga tttggcgctt aaatcattgg ggttgtcgta caccccaatg
     3301 ttattgccga cggtatcgcg aatgtcgttc cactctgaac acgccacctg acaggcttta
     3361 cagccgatac aggtggtaac gtcgatgagt ttcgccactt cttcctggaa gtcccgcgcc
     3421 tgaggcgcgg gggtcagacc gttagtcgcg gaacgacgaa tgatatcttg agattgataa
     3481 gccatatgtc gtctccgtta caccttttcc acattcacaa ggaaggactt aaactccggc
     3541 gtctgcgtgt tcgcatcacc aacgaatggc gttaaagtat tggcaataaa gcctttttta
     3601 gctacgcctt catagcccca gtgaatcgga ataccgatgg tatcgatatc tttgccgttc
     3661 gctttcagcg tgcgaatacg tttggtcacc accgctttgg ctttgatata gccacggttg
     3721 gaggagactt tcacggtatc gccctgggca atgccaagtt tattcgccag cgactccccg
     3781 atttccacaa actgctctgg ctgcaaaatc gcgttcaaca gcgcgtgttt ggtccagtag
     3841 tggaagtgct cggtcagacg ataggtggtt ccgacatacg ggaacttatc ggctttaccc
     3901 aatgcttcgg cgtcgtcttt aaagatacgt gcagccgggt tcgagataac gtttggatgc
     3961 agcgggttag ttcccagcgg cgtttcaaac ggctcgtagt gttccgggaa cggaccttcc
     4021 gccatcttat cgagggtaaa cagacgcccc atgccttcct gctgcatgat aaacggcccg
     4081 acgccgctac ccggaggcgc tgcgctgtag tccggaatat cccagccggt ccacttagtg
     4141 ccgtcccatt tcagcaactg acgcttcgga tcccacgggt taccctgcgg atctgcggag
     4201 gcgcggttat acagaatgcg gcggttaagc ggccatgccc atgcccagcc cagcgtgtta
     4261 ccgaggccag acggatcggc gttatcacgg cgtgccatct ggttgccttc cggcgtccag
     4321 ctaccggcga aaatccagca gccacaggag gttgtaccgt catcgcgcaa ttgggcgaac
     4381 gaactaagtt gttggccttt cttgacgata accgcaccgg ttgccggatc ggtaatatcg
     4441 gccagcgcct taccgttgct ctccatcgcc acttcttctg aagatggctc atgcggaatg
     4501 gcgtagttcc atgtcatgtt cagcacctgg tccgggttcg cgccaccctg ttcggcatac
     4561 atcttgcgca agcgcaggaa gataccggag aggatctcgc cgtcagtcag cgcaatcccc
     4621 ggggcgtccg cacctttcca gtgccactgt aaccagcgac ctgagttaac gatagaaccg
     4681 ttctcttccg cgaagcaggt cgatggcaga cggaacactt cggtctggat cttcgacgag
     4741 tcaacttcgt tcagctcacc gtggttctgc cagaagttag aggtttcagt gttcagtggg
     4801 tcgatggtga cgaggaactt cagtttcgac agacaaccga tcactttgtt tttgttcggg
     4861 aatgaggcaa cagggttaaa gccctggcag atatagccat tgaccttgcc ctctttcatc
     4921 atctcgaagt attgcaggac gtcgtagcct ttatcccact tcggcaacca gtcaaagccc
     4981 cagctatttt ccgccgtcgc tttatcacca aagaaggcct tcatcataga gacgaagaat
     5041 ttcgggtagt tgccccagta gtttacctgg ccttccagca gcggttttgg cgtgttggcg
     5101 gtaaggtagg tttgcagatc ggtctgcttc tcgcttggca gcgtcatgta acctggcagg
     5161 ctctgcgaca gcagccccag gtccgtcagc ccctgaatat tggagtgacc gcgcagggcg
     5221 ttaacgccgc cgcctgccat ccccatattg ccgagcagca gctggatcat cgccatcgta
     5281 cgaatgtttt gcgcaccaac ggagtgttgc gtccagccga gggcatacag gaacgaggca
     5341 gttttatcgt gagcactggt ttctgcgatg tattcgcaga ctttcaggaa cgcgtctttt
     5401 ggcgtaccac agatgttttc aaccacatct ggcgtgtagc gggaaacgtg ctgtttcagc
     5461 aagttccaca cgcagcgcgg atgttgcagc gtggtatcgc gtttggcgaa gccgttttcg
     5521 tccagttcat aagtccagga ggatttatcg tacttgcgtt tttccgcgtc gtagccggtg
     5581 aacaggccat cttcaaagcc gtaatcctca cgcacgatca ggctggcgtt ggtataggct
     5641 tcggtgtatt cgcggttgaa tttttcattg ttcagcaggt acagcaatac gcctgacagg
     5701 aaagcaatgt cagtaccgga acgaataggg gcatagtagt cagctaccgc cgccgtacgc
     5761 gtaaagcgag gatcgatcac aatcagcttc gcgccgttgt gaattttggc ttccatcgcc
     5821 cagcggaacc cgaccgggtg agcttcagcg gcgttaccgc ccatcaccac gacgaggttg
     5881 gcgttcttga tgtcgaccca gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga
     5941 cttgctaccg ttggtccgtg tcagacacgc gcctggttgt cgaccgcgag catacccagc
     6001 gcgcgggaga atttttgcgt taaatagccg gtttcgttac tcgacgcgga agcacacagc
     6061 atcccggtgg agagccagcg gttaacagtc acgccttcgg cgttttgcgc aatgtagtta
     6121 gcatcgcggt cttctttcat cagtttggcg atgcgatcaa acgcctcttc ccaactgatt
     6181 tgctgccatt tatcagaacc tggcgcacgg tattccggaa acttcagacg gctttcggag
     6241 tggatgaaat ccaccaggcc agcgcctttc ggacaaagtg caccgcggtt gaccgggtga
     6301 tccggatcgc cttcgatatg gaagatagat gctttggcgt tttttgctcc gtcaccgagg
     6361 ctgtacatca acagcccaca gcctacggaa caataggtgc aggtattacg ggtttcgcgg
     6421 gtgcgcagca gtttatactg ccgggtttcc gcgagtgcta cgctgggtgc aaaacccagt
     6481 gccgctgccg tggtgcctgc cataccgcca gcgcagatct taaagaactg ccttctgctg
     6541 acctgcatgg attgctcctt gtttcgacat tgtcacgtcc catttacatt cgcttgctgc
     6601 gtgtgcaggg agtgggagtt atttttcttt gcggaagggg ccgcaaaggt ccagaattgg
     6661 ctcaatttcc ctccatccag gaagggtttg taacagaata ccataatgtt ggtgtgtgtg
     6721 ttcttatctg gttaagagaa agtgaaaaaa acacagcgaa aagaaatcga aaatgtgaca
     6781 aatatcacag gtgttcgtca aattgagtta tggcggcgtg atgatttaca acacccacgg
     6841 ttagatgagg tcgcggaaga agttcccgtt gcgctggtct acaacggcat ttcgcatgtg
     6901 gtgatgatgg cgtcgcccaa agaccttgag tactttgcgc tcggtttttc gctttccgag
     6961 gggattatcg aaagtccgcg cgatatcttc ggcatggatg tcgttccttc ctgtaatggt
     7021 cttgaagtac aaattgagct ttccagccgc cgctttatgg ggttgaagga gcgccgccgg
     7081 gcgctggcgg gacgtacggg ctgtggcgta tgcggcgtgg agcaacttaa tgacatcgga
     7141 aaaccggtgc agccgctacc gttcacccag acgtttgatc tcaacaaact ggatgatgca
     7201 ttacgtcatc tcaacgattt tcagccagtg gggcaactga ctggttgtac tcacgccgct
     7261 gcctggatgt tgccatctgg cgaactggtc ggcgggcatg aagacgtggg tcgccatgtg
     7321 gcgctggaca aactgttagg ccgccggtca caagaagggg aaagctggca gcaaggtgcg
     7381 gtactggttt ccagtcgtgc cagttatgaa atggtgcaaa agtcggcgat gtgcggcgta
     7441 gagattttgt ttgcggtgtc tgccgcgacc acgcttgctg tagaagtggc tgagcgctgt
     7501 aatctgacac tggtaggttt ttgtaaaccg gggcgggcaa cggtttatac gcatccgcag
     7561 cgtttga
//
LOCUS       DAOFQD010000186         7496 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.187, whole genome shotgun sequence.
ACCESSION   DAOFQD010000186 DAOFQD010000000
VERSION     DAOFQD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.187"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(20..622)
                     /locus_tag="QBF46_004414"
     CDS             complement(20..622)
                     /locus_tag="QBF46_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ6676517.1"
                     /translation="MNKAVLNNELIAIKAGDITIYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPVTHKAGYAICRSADLNSWEYVPDHRGEIVYNTETGDAKEITALGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHRAAVDAAEAQRQSLIDTAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEE"
     gene            complement(622..1098)
                     /locus_tag="QBF46_004415"
     CDS             complement(622..1098)
                     /locus_tag="QBF46_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDQ6676518.1"
                     /translation="MVIKKVGLGEGSALPVGVPVPWPSATPPTGWLKCNGAAFSAEEY
                     PELAKAYPTNKLPDLRGEFIRGWDDGRGIDTNRSLLSSQGDAIRNIIGALVDVRFNTY
                     PSDSGVFTTSVIGDASSDSIKGGYAKRVTFDASRVVPTANENRPRNIAFNYIVRAA"
     gene            1154..1702
                     /locus_tag="QBF46_004416"
     CDS             1154..1702
                     /locus_tag="QBF46_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6676519.1"
                     /translation="MIYGYARVSTNHQDTELQLTALKSAGCEKIFEEHASGRKSNRPV
                     LKRLIATMQPGDELVVWKLDRIGRNVLHALLMFQQLQEKGINFRSITDGVDLKTASGR
                     YNFRNILSAAQYESDLNSERTLAGLAVARAKGRVGGRRPKFTDEQWREMGERMATGES
                     RQSVSKTYGVGLSTLYKKFPAS"
     gene            complement(1821..2117)
                     /locus_tag="QBF46_004417"
     CDS             complement(1821..2117)
                     /locus_tag="QBF46_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDQ6676520.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            2341..3060
                     /gene="tonB"
                     /locus_tag="QBF46_004418"
     CDS             2341..3060
                     /gene="tonB"
                     /locus_tag="QBF46_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDQ6676521.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(3100..3498)
                     /gene="yciA"
                     /locus_tag="QBF46_004419"
     CDS             complement(3100..3498)
                     /gene="yciA"
                     /locus_tag="QBF46_004419"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDQ6676522.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3603..4142)
                     /locus_tag="QBF46_004420"
     CDS             complement(3603..4142)
                     /locus_tag="QBF46_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDQ6676523.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4172..4915)
                     /locus_tag="QBF46_004421"
     CDS             complement(4172..4915)
                     /locus_tag="QBF46_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDQ6676524.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(4943..5032)
                     /locus_tag="QBF46_004422"
                     /pseudo
     CDS             complement(4943..5032)
                     /locus_tag="QBF46_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5272..5910
                     /gene="ompW"
                     /locus_tag="QBF46_004423"
     CDS             5272..5910
                     /gene="ompW"
                     /locus_tag="QBF46_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDQ6676525.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(5970..6476)
                     /locus_tag="QBF46_004424"
     CDS             complement(5970..6476)
                     /locus_tag="QBF46_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDQ6676526.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQNIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(6522..7022)
                     /locus_tag="QBF46_004425"
     CDS             complement(6522..7022)
                     /locus_tag="QBF46_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDQ6676527.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLAINNVNK
                     KAENKA"
     gene            complement(7108..7287)
                     /locus_tag="QBF46_004426"
     CDS             complement(7108..7287)
                     /locus_tag="QBF46_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDQ6676528.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         2030 a         1742 c         1746 g         1978 t
ORIGIN      
        1 gcaccagata ttgaatggcc tactcctccg gcagttcagg ccagatgaca tccggcgcgg
       61 tgctggtatc tgttgccgtc accgcgtcaa tgtaatccag cacagcgtta agccgggttg
      121 tttctgcctg cgtcagtttc cgcccggcct gtaatttcag ttgaatcaga ctaatggaag
      181 ccattgcagt atcaatcagt gactgacgct gtgcttctgc cgcgtctact gcggcgcgat
      241 gctgtgcctc agtatcggtc acccatttct caccatccca tttatcgtat ggcgttaacg
      301 gggcgatagt ggttgtattt tcggggtaat cacccagagc tgtgatttct ttggcgtctc
      361 ccgtttcggt gttatagacg atttcaccgc gatggtctgg cacatattcc catgagttta
      421 aatctgccga acggcagatt gcataaccag ctttatgtgt aactggtgca tctaaacaag
      481 aacatgccgg tataccgacg ccaaccgcaa gatattcagt tgatgtggaa atatattccc
      541 gcgtttcacc atcataatta taaatggtaa tgtctcccgc ttttatggca atgagttcgt
      601 tatttaatac ggctttattc atcaggcagc cctcacgata taattaaagg caatgttacg
      661 aggtcggttt tcgtttgcag ttggaacaac tctggaagca tcgaatgtta ctcgctttgc
      721 ataaccacct ttaattgaat cagatgaagc atctccgatg acgctggttg taaaaacgcc
      781 agaatcagaa gggtaggtat taaacctgac atccactaat gcaccaatta tatttcgaat
      841 ggcatctccc tgtgacgaaa gcaagctacg gttagtatct attcctcttc cgtcatccca
      901 gccacgaatg aactcaccac gtaaatcagg caatttattt gtcgggtaag cctttgccag
      961 ttccgggtat tcttcagcag aaaaagccgc accgttgcat ttcagccagc ctgttggcgg
     1021 agtggcggaa ggccacggaa caggcacacc aacgggtaat gccgaacctt ctcccaaacc
     1081 aacctttttt ataaccatca aaaatctggt gatgcttcgc cgtttctcct gttttcataa
     1141 caggagaaat cccatgattt acggttatgc ccgagtatca acaaaccacc aggacactga
     1201 attgcaacta acggcgctca agtcagcggg ttgtgagaaa atttttgaag agcatgccag
     1261 cgggaggaaa tcgaatcggc cggttctaaa acggctgatc gccactatgc agccggggga
     1321 tgaactggtg gtctggaagc tggacaggat aggccgcaac gttctgcatg cgctgttgat
     1381 gttccagcag ttacaggaaa agggtatcaa cttccgcagt attaccgatg gcgtggatct
     1441 caaaacagcc agcggccgct ataactttcg taacatcctt tccgcagcac aatatgaatc
     1501 tgatcttaat agcgaacgta ccttagcagg gctggccgta gccagggcaa aagggcgagt
     1561 tggtggtcgc aggcctaagt tcacggatga gcaatggcgg gaaatggggg agcggatggc
     1621 aaccggtgaa tcacgacaaa gcgtatcaaa aacgtatgga gtagggctct caactctgta
     1681 taaaaagttt ccagctagct gataacggga aagaaacaga gaagggcaca aatattgtgt
     1741 actttaatgt gccctttaat ttattgattg gtggttgaat tgtccgtaat tttttgattt
     1801 aagtgcaaat ttctaataaa ttagaacact ttcttaaatg gtttcactga aacgtgttca
     1861 tagactcctg ccgctacgta cgggtcagca tcggcccagg cctgagctgc ttccagcgat
     1921 tcaaattcag caataacggt tgagccagta aatcccgcag cccctggatc gttactgtct
     1981 accgctggca ttggaccagc tgtcaacaaa cgaccttcat catgcagtaa ctgtaaacgt
     2041 gctaaatgtg ccggacgaac ggaaaggcgt ttttcgaggc tatcagcttt gtcttgagcg
     2101 taaataacat acaacacggg cacaactcct tgttcgggaa agttgtaaag tacgttattt
     2161 gaaagggcga agatctgcaa cggaaagatg atgtctttgt taaggccatg cataaagtaa
     2221 gggtaattac gccaaaaatg acattttcac tgatcctgat cgtcttgcct tattgaatat
     2281 gattgctatt tgcatttaaa atcgagacct ggtttttcta ctgaaatgat tatgacttca
     2341 atgacccttg atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat
     2401 ggtgctgttg tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg
     2461 cctgcgcagc cgatttctgt cacgatggtt acgcctgctg atctcgaacc gccacaagcc
     2521 gttcagccgc caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa
     2581 ccgccaaaag aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg
     2641 aagccggtga aaaaggtaca ggagcagcca aaacgcgatg tcaaacccgt agagtcgcgt
     2701 ccggcatcac cgtttgaaaa tacggcaccg gcacgcctga catcaagtac agcaacggct
     2761 gcaaccagca agccggttac cagtgtggct tcaggaccac gcgcattaag ccgtaatcag
     2821 ccgcagtatc cggcacgagc acaggcattg cgcattgaag ggcaggttaa agttaaattt
     2881 gacgttacgc cggatggtcg cgtggataac gtacaaatcc tctcagccaa gcctgcgaac
     2941 atgtttgagc gtgaggtgaa aaatgcgatg cgcagatggc gttatgagcc gggtaagcca
     3001 ggcagtggga ttgtggtgaa tatcctgttt aaaattaacg gcaccaccga aattcagtaa
     3061 gcagaaagtc aaaagcctcc gaccggaggc ttttgactat tactcaacag gtaaggcgcg
     3121 aggttttcct tcaggatcaa ccgcgacata cttaaataat gcttctgtcg ctttatagcg
     3181 ttgcccgatt ggttcagacg ctactttttt cacccacact tcaatattaa tgctgaccga
     3241 tgtcgtccct ttctggacac agcgtgcata gcagcacacc acatcgccga ccgcaaccgg
     3301 ccgtaagaaa gtcattcctt caacccgcac agtcactacg cgaccgtggg caatttcttt
     3361 tgccagaata gcgccgccaa tatccatttg tgacattaac caaccaccaa agatgtcacc
     3421 attggcattg gtatcggcgg gcatggctaa agtacgtaaa acaagatcgc cctgagggac
     3481 gttatgtgtt gtagacatgg taaaaccgac ttaaaaggaa aatcacaggc gcgatgctac
     3541 tatgattttc ctgaggagaa cagaggagat acagcgccag ccccgaagga ctggcagtct
     3601 ggttaggatt tatcttcctg cggcatgtgg cggtagatat agataccgct taacaatgtg
     3661 aagattaagg taagggcggt caggccaaag actttaaagt tgacccaaat attttgcggc
     3721 agccagaatg cgatgtagat gtttgccaga ccgcaaagga taaagaaaac agcccaggcc
     3781 agattcagct tcgaccatac cggttgcggc agcgtgagtt ctttacccag catccgctga
     3841 attagcggct ttttcatcac ccattggctg actaacaggg ctcccgcaaa tagagcataa
     3901 atgactgtaa ccttccattt aataaactca tcattgtgga agaacagcgt caagccaccg
     3961 aagacgacca ccagaacaaa agtgatcagg gccatcttct caaccttacg aaagcgaacc
     4021 cagctataga taagcacaat cgccgtggcg acgatcagcg ccgcagtagc cgcatagatg
     4081 tcataaatct tgtaaaacgc gaaaaagaca accagcggta aaaaatcaag aaactgcttc
     4141 attttacgat tccgtaatca aatgcttaag gttattggcg aatcaacata tacaggcgga
     4201 acagatagat gagcaatatg gctgaaatca ggttgctcaa ggtgttcgcc agtacggcac
     4261 caatttccgg ggttaatgcg gcaaaagaag aggcaaaaag cagcaacagt gtttttgcca
     4321 gcaaccagct cagtactgcg ggtgccacca gacgcatatt cgcccaagtc agccgcatac
     4381 tgctacgcat cgaggcaaaa acgcccattt tgtcctgtac caacatcacc ggtgccaggg
     4441 ccagtaaaat ggccatgata attcccggaa cgaccaccag catgatgcca atctgtacta
     4501 aaagggtagt cagaaaaatc agaataaata actttggcaa tatcggcgca ctggcaccaa
     4561 tagctcgcag cgcactgact ctctgacccg cagacaccag ctggataatt aatattacgc
     4621 ctccggcgag aatggcgtta ccgattaatc ctgaaaaagt ggacgccgct gaagcctgca
     4681 gcaaaatttg ttgctgttcc ggtgacatat tctgaaccag gtcgaacaac ccactactgc
     4741 cgctaacggg cacgccgtca ttgagctgcg caagctgtgc atcactgggt gagaaaacat
     4801 gccctaacac cactgtgata aacgcacata gcaacgatac caacagaatg gtcataaatt
     4861 gattacggaa gaaatttccg gtgtcacggt atacggactg cgccgtgata gacatgcact
     4921 ctccttgagt tttatgcagg tgttaattag cgggcaattg taccctggtt aagcctcggg
     4981 tggcagcatc aggtcttgta tggaaaagca tatctttgta aagcggaggt aattgctggc
     5041 cttcaggcat gggcaaatcg cggcaagtgg cgaccgagtt aatatttgcg tagcgaaaat
     5101 atttaaaaat tgatttaaat cacattaacc aggattctca atgcaacttt taaattaatc
     5161 cagatcaata aagggtgaat tatcatatgt aatgtgatct atgtaggatc atttgttact
     5221 ccaatgtagg tatattcgtc acgtttttat aaccataacg acggagcgga tatgaaaaag
     5281 ttaacagtgg cggctttggc agtaacaact cttctctctg gcagtgcctt tgcgcatgaa
     5341 gcaggcgaat tttttatgcg tgcaggttct gcaaccgtac gtccaacaga aggtgctggt
     5401 ggtacgttag gaagtctggg tggattcagc gtgaccaata acacgcaact gggccttacg
     5461 tttacttata tggcgaccga caacattggt gtggaattac tggcagcgac gccgttccgc
     5521 cataaaatcg gcacccgggc gaccggcgat attgcaaccg ttcatcatct gccaccaaca
     5581 ctgatggcgc agtggtattt tggtgatgcc agcagcaaat tccgtcctta cgttggggca
     5641 ggtattaact acaccacctt ctttgataat ggatttaacg atcatggcaa agaggcaggg
     5701 ctttccgatc tcagtctgaa agattcctgg ggagctgccg ggcaggtggg ggttgattat
     5761 ctgattaacc gtgactggtt ggttaacatg tcagtgtggt acatggatat cgataccacc
     5821 gccaattata agctgggcgg tgcacagcaa cacgatagcg tacgcctcga tccgtgggtg
     5881 tttatgttct cagcaggata tcgtttttaa ttccgcacaa aaacgacccc gtaatatacg
     5941 gggtcaataa ggacatggta taaagggtat tatttcttcg cttctacgcc atcagtttca
     6001 gagcgaatta aaaatttctc agttgtttgc ggaatattct gaatcagcca gtcggccatt
     6061 tgcttttcct catttaaaat cgcttcgatg gttggaattg aagctgtatc accggcattt
     6121 tttgctgctg ctaatagtga ggtgtaacag gcgatttcaa attgctcgaa gacatatccg
     6181 ctaatagagc ctttgactat ttcatcagaa gggaatatac caccgattga ctgcccaagc
     6241 gcagccattt tactcatgga atctttaatg actgaacgtg aaatgtcatt acgatcaaga
     6301 atagtttcca gttgaacaat ctggtttttg gtttcactaa gatgttgttc aatacgagcg
     6361 cgtagttcag gataattatc tatacggctg gccatggatt caagcataga ttcggcttgc
     6421 ttttccattg cgtgggcgtc acgtaaccag tcatgataat gttcaatacg attcatgaaa
     6481 atgtcctctt tataaaatga ctaaaagtta aaattcatat ttcaggcttt attttcggct
     6541 ttcttattta cgttattaat ggccagatca gtcagtttga tgtcggtggc cttttcttct
     6601 tccagggttt ctttcagaag cttcgctgct ttacggtaac ctaattgttc agccagcgtc
     6661 gctaatgtcc cgtaactggc aatctcataa tgctcgactt tctgtgctgc ggcaatcagt
     6721 gcggcatcac gcacttcgtt tttctcggta ctttcgatga cctcattagc ttcttcaata
     6781 agaccttcca ttgccacaca tttcatgcgc ttaattttca ggttcgattc ggactccaca
     6841 acttgatcaa tacgttcaat ctgtccatga gtttcctcga ggtgcgcatg aaaagcctga
     6901 cttaattttt catttgatgt tgctcttgcg agttttgcca gtgcccgggt taattgtttt
     6961 tctgcgctgt aggtatctga aagcaggtga ataaatacat cttcaatggt cttcatattc
     7021 atatttttct ccagtgaaat cacttcgagc catacggccc gcagatgcaa ttatctttat
     7081 cagctgatat taataattaa cggtgaatca ggatttgcct gatttattac caccgctttg
     7141 ttgaccgcct tttttacccg cttcagatgc gcgttgcgga tcatttttaa aattaccgcc
     7201 gctatgctga ccgcctttac ggcctgcgtc ggatgccttc tcacggtctt cggcgaaatt
     7261 tcctgaacca ccacgatgtt cggccatgtt atttctcccg ttgcgttgca ttgtttcatt
     7321 aatatgagtg ttgtgtgtcg acactcatta aaattagtcg ctaatgagaa ttagtcaaat
     7381 taagcgcaac gagaagatag agggaaaata tattttgagg aacattctgg atatattaac
     7441 aattacctga ggaataagtg acttagagaa aattattgat tttactggtg ttatgt
//
LOCUS       DAOFQD010000187         7481 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.188, whole genome shotgun sequence.
ACCESSION   DAOFQD010000187 DAOFQD010000000
VERSION     DAOFQD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.188"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(42..626)
                     /locus_tag="QBF46_004427"
     CDS             complement(42..626)
                     /locus_tag="QBF46_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="HDQ6676529.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLS
                     RLIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMI
                     HEMRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKA
                     PLADISRCDGKPCVKVDTRATYGDKENTWMIIKK"
     gene            complement(639..1736)
                     /gene="mobA"
                     /locus_tag="QBF46_004428"
     CDS             complement(639..1736)
                     /gene="mobA"
                     /locus_tag="QBF46_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="HDQ6676530.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGN
                     MPAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDR
                     HAYQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKA
                     RFGETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAG
                     QVQRFDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKP
                     EPAQEDTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMER
                     LAEKQRQQEKEQQRLAEQTRQKPDKGWSFSR"
     gene            1902..2369
                     /gene="traD"
                     /locus_tag="QBF46_004429"
     CDS             1902..2369
                     /gene="traD"
                     /locus_tag="QBF46_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HDQ6676531.1"
                     /translation="MSKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQD
                     IKTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAG
                     LVDSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
     gene            2962..3912
                     /locus_tag="QBF46_004430"
     CDS             2962..3912
                     /locus_tag="QBF46_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDQ6676532.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     gene            3920..4333
                     /locus_tag="QBF46_004431"
     CDS             3920..4333
                     /locus_tag="QBF46_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676533.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTS
                     ELLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHI
                     DSLKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
     gene            4580..4897
                     /locus_tag="QBF46_004432"
     CDS             4580..4897
                     /locus_tag="QBF46_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDQ6676534.1"
                     /translation="MFNVILHDDVPNELAGLPPVVRAKMIRLIDKLKTNATALREPDS
                     KPLRDGLFELRTMGTDIARGLYVYQKGRNIYLLRVFIKKTQKTPSGEISMAFERLKEM
                     LNE"
     gene            4890..5177
                     /locus_tag="QBF46_004433"
     CDS             4890..5177
                     /locus_tag="QBF46_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6676535.1"
                     /translation="MSKVKGIAWEHVRNELMSNPEVQAAYEAEERKERLQAMLAEWRH
                     HAGLTRAQVAERMGVTPPTVSRMESNVIKASIETLARYARACGVKHPQITL"
     gene            complement(5515..7140)
                     /locus_tag="QBF46_004434"
     CDS             complement(5515..7140)
                     /locus_tag="QBF46_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011638903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase
                     MCR-4.6"
                     /protein_id="HDQ6676536.1"
                     /translation="MISRFKTLSVNQFTFITALFYVAIFNLPLFGIVRKGIEKQPEVD
                     PLFIASMPLFLTFALSFLFSIFTVKYLLKPFFIVLTLLSSSVFFAAYQYNVVFDYGMI
                     ENTFQTHPAEALMYVNLASITNLLLTGLLPSYLIYKADIHYQPFFKELLHKLAFMLLM
                     FVGIGIVAFFYYQDYAAFVRNNSELRRYIVPTYFVSSASKYLNEHYLQTPMEYQQLGL
                     DAKNASRNPNTKPNLLVFVVGETARSMSYQYYGYNKPTNAHTQNQGLIAFNDTSSCGT
                     ATAVSLPCMFSRMGRADYDPRRANAQDTVIDVLSHSGIKVQWFDNDSGCKGVCDQVEN
                     LTIDLKSDPKLCSGQYCFDQVLLNKLDKILAVAPSQDTVIFLHIIGSHGPTYYLRYPP
                     EHRKFIPDCPRSDIQNCSQEELINTYDNTILYTDFILSEVVNKLKGKQDMFDTAMLYL
                     SDHGESLGEKGMYLHGAPYSIAPKEQTSVPMLAWVSNDFSQDNQLNMTCVAQRAEQGG
                     FSHDNLFDSLLGLMNVKTTVYQSQLDIFAPCRY"
BASE COUNT         2222 a         1646 c         1643 g         1970 t
ORIGIN      
        1 taattcacga caacaataac ggcgcaatgt gcgccgttat gttatttttt gataatcatc
       61 caggtattct ctttatcacc atacgtagct cgggtgtcca ccttaacgca gggctttccg
      121 tcacaacgcg atatatcagc cagcggggct ttcttctgcc agaccgcttc catccgttgc
      181 atttcagcaa tctggctgta cccgtcattc ataaaccacg taaatgccgt cacgcaggca
      241 gccatgacga aaaatatcgt cagcacgaga taaatcgcgg atttccacgt atagcgcgac
      301 atctcgcgtc gcatttcatg gatcatcgcc ttcgccgtat cggtagcctg attaagcgct
      361 tctgtcgccg gtttctgctg tgctaatccg gcttgtttca gctctttctc aacctgaatg
      421 agcgcggcat gaacagattg cctgacggtt tcagtcatat taccggacgc gctgtccagc
      481 tcacgaatga gcctgctcag cgtgtcactc tgctgctgta attgtgctga ggcgtcctga
      541 aactgctcag tcagagaggt aacacgcttt tccagcgcct gatgatgccc gatgagggca
      601 gcgatttgtt taatttcgtc gctcatgaaa aatcctgtct atcgtgaaaa tgaccagcct
      661 ttatccggct tctgtcgtgt ctgttcggcg agtctctgct gttctttctc ctgctgacgc
      721 tgtttttctg ccagtcgctc catttcctga tgtatcccct gaaactccgc cattgtctcg
      781 ttcacaaggg actgaaggtc gatttcgtcc tgtacgtcct tcagcgcatc actgaccagt
      841 gcgttcagct tctccggctc tttctcaaaa tcgaaaaccc tgccggaagt gtcttcctgc
      901 gcaggttcag gcttctgctc ttgttttatc gtcaggttat cccgctcgct gatggcttcc
      961 cgtagcgagg tggtcacgtc aatcacactg tcacgttcat cacgagactg ctccgcctgc
     1021 ctttcagcgt ccctgcgctc aaggatggcc tgtaactgcg cagtatcgaa ccgctgaacc
     1081 tgtcccgcgc caagatgccg ctcaggctca cgctcaatgc cctgagcttt gagtgagcgt
     1141 gcatcaacgc ggtcggtatg ctgataccgt tcaaggtgct tattctgtag gtcagcccag
     1201 cgttcccgct gggcgacgag gtgctctttg cgttctgtcg gcgtttcccc gaaacgtgcc
     1261 tttttcgcac cgccgcgctc aggggctttg ctgttagctc gcttaaaata ctgctgcggg
     1321 tcacggtcga taccgtcatt catccgctca gagaacatga tatgcgcgtg cggctgctcg
     1381 ccgccggcta tcgccgcttt cggattatga atggcgaact gatacgcatg acggtcgccg
     1441 atttcctgcc ggacaaaatc gcggaccagt tcaaggcgtt gttcgggttt cagctcacgc
     1501 ggcagggcaa tctcgatttc acggtaggta cagccgttgg cacgttcaaa cgtgtcagcg
     1561 gctttccaga actccgtcgg tttatgcgct gcccaggcag gcatattgcc ggactccttg
     1621 tgctcaaggt cggagtcttt ttcacgggca tactttccct cacgcgaaat ataatcggca
     1681 tggggagagg cactgccttt cccgccggtt tttacgctga ggtgatagga ggccatcgtg
     1741 tttttatccc gctgaaggcg cgcaccgttt ctgaacgaag tgaagaaacg tctaagtgcg
     1801 ccctgataaa taaaagagtt atcagggatt gtagttgaat ttgacctcca ctgccatcaa
     1861 tgagcataat tcccccgtta aaactcagag ggtaaacggg catgagtaaa agcgaaaaac
     1921 aggaacaatg ggcagcagaa agagtgcagt atattcgcgg cttaaagtcg ccgaatgagc
     1981 aacagaaact tatgctgata ctgacggata aagcagataa aacagcacag gatatcaaaa
     2041 cgctgtccct gctgatgaag gctgaacagg cagcagagaa agcgcaggaa gccagagcga
     2101 aagtcatgaa cctgatacag gcagaaaagc gagccgaagc cagagccgcc cgtaaagccc
     2161 gtgaccatgc tctgtaccag tctgccggat tgcttatcct ggcgggtctg gttgacagta
     2221 agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc agtctgaatg
     2281 acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag gaactgctga
     2341 acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc tgagaagccc
     2401 gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa aaggcgcctg
     2461 tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg aatgtcacga
     2521 aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca gcgatttgcc
     2581 cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt gtagaggagc
     2641 aaacagcgtt tgcgacatcc tttttaatac tgcggaactg actaaagtag tgagttatac
     2701 acagggctgg gatctattct ttttatcttt ttttattctt tctttattct ataaattata
     2761 accacttgaa tataaacaaa aaaaacacac aaaggtctag cggaatttac agagggtcta
     2821 gcagaattta caagttttcc agcaaaggtc tagcagaatt tacagatacc cacaactcaa
     2881 aggaaaagga ctagtaatta tcattgacta gcccatctca attggtatag tgattaaaat
     2941 cacctagacc aattgagatg tatgtctgaa ttagttgttt tcaaagcaaa tgaactagcg
     3001 attagtcgct atgacttaac ggagcatgaa accaagctaa ttttatgctg tgtggcacta
     3061 ctcaacccca cgattgaaaa ccctacaagg aaagaacgga cggtatcgtt cacttataac
     3121 caatacgctc agatgatgaa catcagtagg gaaaatgctt atggtgtatt agctaaagca
     3181 accagagagc tgatgacgag aactgtggaa atcaggaatc ctttggttaa aggctttgag
     3241 attttccagt ggacaaacta tgccaagttc tcaagcgaaa aattagaatt agtttttagt
     3301 gaagagatat tgccttatct tttccagtta aaaaaattca taaaatataa tctggaacat
     3361 gttaagtctt ttgaaaacaa atactctatg aggatttatg agtggttatt aaaagaacta
     3421 acacaaaaga aaactcacaa ggcaaatata gagattagcc ttgatgaatt taagttcatg
     3481 ttaatgcttg aaaataacta ccatgagttt aaaaggctta accaatgggt tttgaaacca
     3541 ataagtaaag atttaaacac ttacagcaat atgaaattgg tggttgataa gcgaggccgc
     3601 ccgactgata cgttgatttt ccaagttgaa ctagatagac aaatggatct cgtaaccgaa
     3661 cttgagaaca accagataaa aatgaatggt gacaaaatac caacaaccat tacatcagat
     3721 tcccacctac ataacggact aagaaaaaca ctacacgatg ctttaactgc aaaaattcag
     3781 ctcaccagtt ttgaggcaaa atttttgagt gacatgcaaa gtaagtatga tctcaatggt
     3841 tcgttctcat ggctcacgca aaaacaacga accacactag agaacatact ggctaaatac
     3901 ggaaggatct gaggttctta tggctcttgt atctatcagt gaagcatcaa gactaacaaa
     3961 caaaagtaga acaactgttc accgttacat atcaaaggga aaactgtcca tatgcacaga
     4021 tgaaaacggt gtaaaaaaga tagatacatc agagctttta cgagtttttg gtgcattcaa
     4081 agctgttcac catgaacaga tcgacaatgt aacagatgaa cagcatgtaa cacctaatag
     4141 aacaggtgaa accagtaaaa caaagcaact agaacatgaa attgaacacc tgagacaact
     4201 tgttacagct caacagtcac acatagacag cctgaaacag gcgatgctgc ttatcgaatc
     4261 aaagctgccg acaacacggg agccagtgac gcctcccgtg gggaaaaaat catggcaatt
     4321 ctggaagaaa tagcgctttc agccggcaaa ccggctgaag ccggatctgc gattctgata
     4381 acaaactagc aacaccagaa cagcccgttt gcgggcagca aaacccgtac ttttggacgt
     4441 tccggcgggt ttttgtggcg agtggtgttc gggcggtgcg cgcaagatcg cttatgttaa
     4501 atacagcaga aaaaccataa aattacccta taacgcaaat tgctttttat gctaatattt
     4561 cgcaatggag ggcgaaccag tgtttaacgt catattgcac gatgatgtac caaatgagct
     4621 ggctggctta ccgccagtag tcagagcgaa gatgattcgc ctgattgata aacttaaaac
     4681 caacgctact gcattacgag agccagatag caaaccactc cgggatggat tatttgagtt
     4741 aaggacgatg gggacggata ttgccagagg tctttatgtt tatcagaaag gcaggaatat
     4801 ctatctactt agagtcttta ttaaaaagac tcagaaaacc ccatcgggtg aaatatctat
     4861 ggcctttgaa cgtcttaagg agatgctaaa tgagtaaagt taaaggtatc gcttgggagc
     4921 atgttaggaa tgaattgatg tctaatcctg aggttcaagc agcctatgaa gcagaggagc
     4981 gcaaggaaag acttcaagct atgctcgctg aatggcgtca tcacgcagga ttaacaagag
     5041 cccaggtggc agaacgcatg ggtgttactc cacctaccgt ttctcgcatg gaatctaatg
     5101 tgattaaggc tagcattgaa acactggctc gatacgcaag agcatgcggt gttaagcacc
     5161 ctcagatcac gttgtaattt tcaaggccgc tttaagcagc cttttgatgc gtgcaagatc
     5221 gcttatgttg aacaaggagt ttttttgcaa aaaaccatca tttcacacat atgagataat
     5281 cctaagggat ataaataggc taattttgcc caacgcgcca aaaatgtaaa gttagcattt
     5341 acagcttgta agccattgtt ctagtttcac aacggcaaat tgatgttaag tctaaaagac
     5401 cgtgataact cattgtggaa gattgagagc taaatgtaac aatagaggac attgttagtc
     5461 caagatttaa ggggtgaata tgtcacccct aagataaaac atttacagcc atatctaata
     5521 cctgcaaggt gcaaaaatat cgagttggct ctgatagacg gtggttttta cattcataag
     5581 tcctagcaaa ctgtcgaaca aattgtcgtg ggaaaagccg ccctgttctg ctcgctgtgc
     5641 aacacaagtc atgttcaact gattatcttg gctaaagtca ttagataccc aagccagcat
     5701 tggtacgcta gtttgttctt tcggtgcaat actatagggc gcaccatgta aatacatgcc
     5761 cttttcaccc aaagactcac catggtcaga gagatacagc attgcagtat cgaacatatc
     5821 ctgcttacct tttaatttat tcaccacttc actgagaata aaatccgtat atagaatagt
     5881 gttgtcgtag gtgttaatca gttcttcttg actgcaattt tgaatatcac tgcgcggaca
     5941 atccggtata aatttacgat gctctggcgg gtatctaaga taataagttg gtccatgact
     6001 accaatgata tgcaaaaaaa ttactgtatc ttgacttggt gctactgcca gaattttatc
     6061 taatttgttg agcaatactt ggtcaaaaca atattggcca gaacacagct tcggatcact
     6121 cttcaaatct atcgtgagat tttcaacctg atcacacaca cctttacagc cagaatcatt
     6181 atcaaaccac tgtactttta taccactatg acttaacaca tcaatcactg tgtcttgagc
     6241 attagcacgg cgaggatcat agtctgcccg ccccattcgt gaaaacatac agggtagaga
     6301 caccgccgtg gccgtgccgc atgagctagt atcgttaaac gcaatcagcc cctgattttg
     6361 ggtatgagca ttggttggct tgttatatcc ataatattga tagctcattg agcgcgcagt
     6421 ttcacccaca acaaacacta ataagttagg tttagtgttc gggttacgac tggcattctt
     6481 cgcatctagg ccaagttgtt ggtattccat gggcgtctgc aaatagtgct cattgagata
     6541 tttagatgca ctactgacaa aataggtagg gacaatgtaa cgccttaact cactgttgtt
     6601 tcgaacaaat gcagcataat cttgatagta aaaaaaggcg actatcccaa tgccaacgaa
     6661 cattagcagc ataaaggcta atttatgcaa taactcctta aaaaagggct gataatgaat
     6721 atcggcctta taaataagat atgacggtaa tagcccagtc agcagtagat tggtaattga
     6781 tgcaagattt acatacatca atgcttcagc aggatgtgtt tgaaacgtgt tttctatcat
     6841 gccgtagtca aacacgacat tgtattgata ggctgcaaaa aatacacttg aggaaagtaa
     6901 cgtcaatacg ataaaaaagg gcttcagcag gtatttgacg gtaaaaattg aaaacaaaaa
     6961 actcagcgca aatgttaaaa atagcggcat agatgcgatg aaaaggggat caacttctgg
     7021 ttgtttttca attccttttc gcactatacc aaagagcggt agattgaaaa tggcaacata
     7081 aaacaacgca gtgatgaaag tgaattggtt aaccgataac gtcttaaatc tagaaatcac
     7141 aaaaacccct tttttgctaa aaaaaacaat actagcttga cctcattaaa ataaccttat
     7201 gattaaaagg ttattttaat gagtcaccaa caaacttaac agataagacc gttttaggtg
     7261 aattgagccc aattcaccta aaagttacat gtaaacaatt aagtaaataa acttcaatta
     7321 cttataagtt tacttgtaat ttcttgtgtg ctcttcacag aattcagtgc ccgataaata
     7381 gaggcttctc caaggtgata ctccttggca agctgcccaa tggtggcccc ctcagctctt
     7441 ttatcataaa tagcctgttt taaggtatcc gtgagtttta c
//
LOCUS       DAOFQD010000188         7381 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.189, whole genome shotgun sequence.
ACCESSION   DAOFQD010000188 DAOFQD010000000
VERSION     DAOFQD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.189"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(389..1525)
                     /locus_tag="QBF46_004435"
     CDS             complement(389..1525)
                     /locus_tag="QBF46_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ6676537.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPSTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1528..1890)
                     /locus_tag="QBF46_004436"
     CDS             complement(1528..1890)
                     /locus_tag="QBF46_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDQ6676538.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2427..4340
                     /gene="mtlA"
                     /locus_tag="QBF46_004437"
     CDS             2427..4340
                     /gene="mtlA"
                     /locus_tag="QBF46_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDQ6676539.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4570..5718
                     /gene="mtlD"
                     /locus_tag="QBF46_004438"
     CDS             4570..5718
                     /gene="mtlD"
                     /locus_tag="QBF46_004438"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDQ6676540.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5718..6305
                     /gene="mtlR"
                     /locus_tag="QBF46_004439"
     CDS             5718..6305
                     /gene="mtlR"
                     /locus_tag="QBF46_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDQ6676541.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6317..6526)
                     /locus_tag="QBF46_004440"
     CDS             complement(6317..6526)
                     /locus_tag="QBF46_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676542.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6811..7173
                     /locus_tag="QBF46_004441"
     CDS             6811..7173
                     /locus_tag="QBF46_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDQ6676543.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT         1908 a         1846 c         1847 g         1780 t
ORIGIN      
        1 aatacgataa ttaaaagtga agaagaaaat agttgtatac atatattcca tgaccctaca
       61 ttgtgatcta tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc
      121 atcatcgcaa tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg
      181 aacaactcat atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa
      241 gacggataaa aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt
      301 aaagaaaaat catcgcagct aatgaagtaa tatgcaaaaa taattagtcc ttacaaggag
      361 caaagttcca taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccagc
      421 tggtcattct tagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa
      481 ccgccacctg ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt
      541 caatggttcc cagcacaccg tccgtgccgg gcacgaccgt taatgattgc aataccccct
      601 gcgcctgata agaaccgcct ggcacgacag gtaaaatact ggtcagtttg ccgtggaaca
      661 cctgcccagg tagcgcgtta aacaccactt ctgcatcatc accaggtttc agacgtaaca
      721 gcgagttttg ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aaaaccatca
      781 ccggacgcaa cggcaaggca gctgcgtatg tgcttgggcg gatcagtacc tgagtgacgt
      841 agccattgct tggtgcgcga atgacagtct gctcaagatt atattttgct tcagtaagtt
      901 gcgctcttaa gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct
      961 ggatctgcgc ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt
     1021 gccgcgcatc gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca
     1081 gataacgttg ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg
     1141 cctgcgcttc tgtgagctgc gcgcgcagag tctttatatt atgcgtcgcc gtcatcaggt
     1201 cagcctgaag cctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct
     1261 cacccttttg aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct
     1321 gtggcgtgat ggggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt
     1381 agttcatcaa caaaatcaaa ccactcacca gaaacacgcc acccagcgcc gccgtcgcca
     1441 gcgtccactg attcaccgga atacgaaaga ttttaaagac cgcccacgcc agcgccacgt
     1501 aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt
     1561 tttctcggcg gcgagttgat gctccagccg ggaaattcgg tcagaaagtg cggcgatttc
     1621 cgggtcagtc gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg
     1681 ttgatagagc gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct
     1741 cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt tggcaatcag
     1801 ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag
     1861 cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt
     1921 gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg
     1981 cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct tagttacata
     2041 acgattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa
     2101 atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacacta
     2161 ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt tacacaagcg
     2221 ttttgtgatg aacgtcacgt caattacctc tctaccccct atatttatgt gattgatatc
     2281 acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac
     2341 aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga
     2401 cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct
     2461 ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta
     2521 tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg
     2581 tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg
     2641 taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg
     2701 cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta
     2761 agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata
     2821 acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc
     2881 cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg
     2941 acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg
     3001 ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat
     3061 caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca
     3121 tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc
     3181 tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg
     3241 cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg
     3301 tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt
     3361 acttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta
     3421 ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta
     3481 tgcaggacat gaaagctgag tctaaaggcg catctccgct gtctgctggc gatgtgacta
     3541 acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttccagtg
     3601 cgatgggcgc aggcgttctg cgtaagaaaa ttcaggatgc aggtctgtcg cagatttctg
     3661 ttactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg
     3721 acctgaccga acgcgctatg cgccaggttc cgcaggcaca gcatatttcg ctgaccaact
     3781 tcctcgacag cggcctgtac accagcctga ccgaacgtct ggttgctgcc caacgccaca
     3841 cggcaaacga agagaaagta aaagacagcc tgaaagacag ctttgacgat tccagtgcta
     3901 acctgttcaa gctaggcgcg gagaacatct tcctcggtcg caaagcggca accaaagaag
     3961 aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggaatacg
     4021 ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg
     4081 tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtc gtgttctgcc
     4141 agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt ctggtgattg
     4201 gtattgctgc ccgtaacaac gagcacattc aggttatcac cagcctgacc aatgcactgg
     4261 atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac
     4321 tgctggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg tggggttaat
     4381 tgcctgatgc gctacgctta tcaggcctac aggatgcatc acaatttgtt gaatttgcac
     4441 gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc tcctcacgga
     4501 gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag
     4561 gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt
     4621 aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt
     4681 gatgccctga atgcccgtca tagctatcag gtacatgtgg ttggtgaaac cgagcaggta
     4741 gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt tgatctgatt
     4801 gctcaggttg atttagtcac taccgccgtt ggcccggttg tgctggaacg tattgctccg
     4861 gcaatcgcca aagggctggt gaaacgtaaa gaacaaggta atgaatcccc gctgaacatc
     4921 atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt gatgaacgcc
     4981 ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga ttccgccgtt
     5041 gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt gacggtagaa
     5101 accttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc gaacatccca
     5161 ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt caccctgaac
     5221 acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac cattcgtgac
     5281 gcgattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgca
     5341 gtattgatca agcgctacgg ctttgacgct gacaagcatg cggcgtacat ccagaaaatt
     5401 ctcggccgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagcca
     5461 ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac tgctcggtac gctggaatat
     5521 ggtctgccac ataaaaacct gattgaaggt attgccgctg caatgcactt ccgcagtgaa
     5581 gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc gcaggcggcg
     5641 ctggcacaga tttccggtct tgatgccaac agcgaggttg tatccgaggc ggtaaccgct
     5701 tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg aataacagat
     5761 tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc
     5821 gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag ctcctgaccg
     5881 aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg
     5941 ctgtagaacc gttactcgac ggcgatggtc cgctgggcga tctttctgtg cgtttaaaac
     6001 tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggaa ctgctgatgg
     6061 cattgcgtga agagctaaat cacgacggca acgagtacgc ctttaccgac gacgaaatcc
     6121 ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca cagtttgaac
     6181 cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa
     6241 caatggtcct ttcactgact gagctgattt ccaaaatcag cttaaaaaaa gcctttcaaa
     6301 agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat
     6361 actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac ggaatcgcgt
     6421 tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc atgaaatcta
     6481 cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt
     6541 attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg gtaaagatga
     6601 cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt atcttttttc
     6661 tatagatttc ttaattaatc aacgaattgc gtagtttttg agcatgttaa tgattacgtt
     6721 acagaactat tacaatgtca ttagttgtga acaaagcacc tggtcgcgca tactaggggc
     6781 tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa
     6841 cgtctcagcg atcgcctgga cgccatccgc caccagcagg ccgatctttc gctggttgaa
     6901 gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct
     6961 cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag cacaaaaact gatgaagatg
     7021 ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt
     7081 gttcgcggac tggtggttgt gcacccaatg accgcactgg gccgtgaaat gggcctggaa
     7141 gagatgaccg ggttttcaaa gaccgcgttt taagcccctc agacgactgg tacgatcgta
     7201 tcaacccgca tctgacttgc gttagctggt caatgcgggg actctcctgt ctactcgcct
     7261 aatgaccaaa caattcactc cccttacgtc gatttaccgc tggttgatat tctgaaaatg
     7321 catctttacc gaaataacca cctataaaca ataaaaatat atagaagata tcatttatta
     7381 a
//
LOCUS       DAOFQD010000189         7172 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.190, whole genome shotgun sequence.
ACCESSION   DAOFQD010000189 DAOFQD010000000
VERSION     DAOFQD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.190"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            179..790
                     /gene="ruvA"
                     /locus_tag="QBF46_004442"
     CDS             179..790
                     /gene="ruvA"
                     /locus_tag="QBF46_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDQ6676544.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            799..1809
                     /gene="ruvB"
                     /locus_tag="QBF46_004443"
     CDS             799..1809
                     /gene="ruvB"
                     /locus_tag="QBF46_004443"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDQ6676545.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1956..2741)
                     /gene="znuB"
                     /locus_tag="QBF46_004444"
     CDS             complement(1956..2741)
                     /gene="znuB"
                     /locus_tag="QBF46_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDQ6676546.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2738..3493)
                     /gene="znuC"
                     /locus_tag="QBF46_004445"
     CDS             complement(2738..3493)
                     /gene="znuC"
                     /locus_tag="QBF46_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDQ6676547.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3572..4504
                     /gene="znuA"
                     /locus_tag="QBF46_004446"
     CDS             3572..4504
                     /gene="znuA"
                     /locus_tag="QBF46_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDQ6676548.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4520..5842
                     /gene="mepM"
                     /locus_tag="QBF46_004447"
     CDS             4520..5842
                     /gene="mepM"
                     /locus_tag="QBF46_004447"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDQ6676549.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5962..6933
                     /gene="lpxM"
                     /locus_tag="QBF46_004448"
                     /gene_synonym="msbB"
     CDS             5962..6933
                     /gene="lpxM"
                     /locus_tag="QBF46_004448"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDQ6676550.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
BASE COUNT         1889 a         1755 c         1843 g         1685 t
ORIGIN      
        1 cactgaatat gtaatattaa aatatttgct tccaaaataa cctgtagaat aaattatact
       61 gtgccatttt tcagatcatc gagacacctc gcaagttttc ttcatccttc gctggatatc
      121 tatccagcat ttttttatca tacagcatta tctttgattc attacgcagg agcgtcatgt
      181 gataggcaga ctcagaggca tcatcattga aaaacaaccc ccgctggtgt taattgaagt
      241 gggcggcgta ggctatgaag tgcatatgcc gatgacctgt ttttatgaac tccctgaagc
      301 gggacaggaa gcgatcgttt tcacccactt tgtggtgcgt gaagacgcgc agctgctgta
      361 cggttttaac aataaacaag agcgcacgtt gttcaaagag ttgatcaaaa ccaacggcgt
      421 cggcccgaag ttggcgctgg cgatcctctc cggaatgtca gcgcagcagt tcgttaatgc
      481 cgttgagcgt gaagaagtgg gggcgctggt gaaactgccg ggtattggca aaaaaaccgc
      541 cgaacgcttg attgttgaaa tgaaagaccg atttaaaggt ctgcatggcg atctctttac
      601 gccagccgcc gacctggtac tcacatcacc tgccagcccg gcgaccgacg atgctgaaca
      661 ggaagcggtt gccgcgctgg tggcgctggg ctataaacca caagaagcaa gccgcatggt
      721 gagcaaaatc gctcgccctg acgccagcag tgaaacatta attcgcgaag ccctacgcgc
      781 cgcgttatga ggtaaaggat gattgaagca gaccgtctga tttctgccgg taccactttg
      841 ccggaagatg tggcagatcg cgccattcgc cctaaattac tggaagagta tgttggtcag
      901 ccgcaggttc gttcacagat ggagattttc atcaaagcag cgaaactgcg cggcgatgcc
      961 ctcgatcatc tgttgatttt tggtcctccg gggttgggta aaactacgct tgccaacatt
     1021 gtcgccaatg aaatgggcgt taatttacgc acgacttctg gtccggtgct ggaaaaggcg
     1081 ggcgatctgg cagcgatgct cactaacctt gaaccgcatg acgtgctgtt tattgatgag
     1141 atccaccgtc tatcgccagt tgttgaagaa gtgctgtacc cggcaatgga agactaccaa
     1201 ctggatatca tgattggtga aggtccggcg gcacgctcca ttaaaattga tttgccgccg
     1261 tttaccctga ttggtgcaac cacgcgcgca ggttcgctga catcaccgtt gcgcgaccgt
     1321 tttgggattg tgcaacgtct ggagttttat caggtgccgg atctgcaata tatcgtcagt
     1381 cgcagcgcac gctttatggg gcttgagatg agtgatgacg gggcgctgga agttgctcgt
     1441 cgcgctcgcg gtacgccgcg cattgccaac cgtctgctgc gtcgagtgcg tgatttcgcc
     1501 gaagtgaagc acgatggcac catctcggca gatatcgctg ctcaggcgtt ggatatgttg
     1561 aatgtcgatg ctgaaggttt cgattatatg gaccgcaagt tattgctggc ggtaatcgat
     1621 aagttctttg gtgggccggt aggtctggat aaccttgcgg cagccattgg cgaagaacgt
     1681 gaaaccattg aggatgtgct ggaaccttat ttgattcagc aaggcttttt gcagcgtaca
     1741 ccgcgtgggc gtatggcgac gacgcgggcg tggaatcact ttggcataac gccgccagaa
     1801 atgccgtaag tcggattggt cgctcgcacc gcatccgaaa ttggcagtgt gcctgatgcg
     1861 acgctattcg cgtcttatcc ggccagccag agatagcgaa ccgtaggtcg gataaggcgc
     1921 tcgcgccgca tccgacaaat gtgttcagcg atagattagc tggcctgctt tttcatcata
     1981 ctgagaataa ataacagtgc cgcacatagc accaccgacg gaccagccgg cgtatcgtaa
     2041 aatgcggaaa aggttaaacc gccagtcact gccaccatcc ccaccaaaac agcgacacca
     2101 gccatctgtt ccggcgtacg ggcaaagcga cgcgcagtag ctgcaggaat aatcagcaac
     2161 gaagtaataa tcaacgcacc gacaaatttc atcgctacac caatcgtcaa tgccgtcacc
     2221 agcatcaaca acaatttcac tcgctgtaat ttcacaccat caacaaacgc cagatccggg
     2281 ctaatcgtca tcgacagcaa attgcgccat tgccagaaca aaatagccac cacgatgacc
     2341 acgccaatcg caatagagat gagatcttct ggcgtcactg ccagtaaatc accgaacagg
     2401 taagccatca aatcaacacg aatgttagac atcagactca cgaccaccag gcccagcgac
     2461 agggcactgt gcgccataat ccctaataat gtgtcgatcg ccagctgtgg acgcttctcc
     2521 agccatacca gaccgcccgc cagcagcagc gtaacggcaa ttaccgcata gaatggattc
     2581 acgtccagca acaaaccaaa cgcgacacca agtaatgagg catgagccag cgtatcaccg
     2641 aaataagaca tacgacgcca gactacaaac gaacccagcg gacccgcggc acaggcgagc
     2701 atgatcccgg ctaaccaacc gggaaataat aattcaatca tgagcgatca tttccccgac
     2761 gcaaaacaat tcgtccctgt aaatcgtgac gatgattatg atgatggcga tagataccca
     2821 gttgttcagc accacgagga ccaaacattg aaataaactc cggatgcagg gaaacaactt
     2881 ccggtgtgcc ggaacaacaa atgtggtgat tcaggcacag cacttcatcg gtttttgcca
     2941 tcaccagatg cagatcgtga gaaaccatta aaacgccaca atccagttcg cgacgcaact
     3001 ggtcaataag gtcatataac gccacctggc catttacatc cacgccttga gtgggttcat
     3061 ccagcactaa taattgcggt cgatttaaca atgctcgcgc taacagtaca cgctgtgttt
     3121 cgccaccaga gagcttttgc atcggtgcgt taatcaggtg cccggcctgg acacgtttca
     3181 gtgcaggcaa aatatcttct ttatgtgtac cagggcgtaa gcgtaaaaaa cggtttacgg
     3241 tcagtggcaa cgtggtgtcg agatacagct tctgcggtac atagccgatg cgcagttttc
     3301 cgttgcgctt gataacccct tcatcgggtg ttaccagccc gagcactacc cgtaccagtg
     3361 tcgacttacc tgcgccattt ggtccaagta aagtcaaaat ttttccaggt ttaagttcca
     3421 gcgacacatc agagaggacg cggcgttggc caaaagaaac cgagacattt tccagggaaa
     3481 ccagacttgt catgttaatt ttagtcttgc agtagtcatg aaatgttata atatcacact
     3541 tctcatattc attacgatga ttggtcgcat tatgttacat aaaaaaacgc ttcttttcgc
     3601 agcattatcc gccgctctct ggggaggtgc aacacaggcc gcagatgccg ccgttgtcgc
     3661 ttcgcttaaa cccgttgggt tcatcgcttc tgccattgct gatggtgtaa cagaaacgga
     3721 ggttttactg cctgacggcg cttcagaaca tgattattca ctgcgtccat cggatgtaaa
     3781 acgcttacag aacgcggact tagtcgtttg ggttggcccg gagatggaag cgtttatgca
     3841 aaaaccggta agcaaattac cagaagcgaa gcaggtaacg attgcgcagc ttgaagatgt
     3901 gaaaccgcta ctgatgaaaa gtattcacgg cgatgatgat gatcacgacc acgcggaaaa
     3961 aagtgacgaa gatcaccatc acggcgattt caacatgcat ctttggcttt ccccagagat
     4021 agcgcgggct acagcggttg caatccatgg aaaattagtg gaacttatgc cgcaaagtcg
     4081 agccaaactt gacgccaacc tgaaggattt tgaggcacaa ttagcctcaa ccgaaacgca
     4141 ggttggtaac gagctcgcgc cgctcaaggg gaaaggttat ttcgtttttc acgatgctta
     4201 cggctatttc gaaaaacagt ttggactgac accgcttggt cattttaccg ttaaccctga
     4261 gattcaacct ggcgcgcagc gtttacatga aataagaaca cagttggttg agcaaaaagc
     4321 aacctgcgtt tttgctgagc cacagttcag gccagcggtc gttgagagcg tcgcacgagg
     4381 gacatccgtt cgtatgggaa cgttggatcc cctggggacg aatatcaaac tgggtaaaac
     4441 aagctattca gaattcctga gtcaattagc caaccagtat gcgagctgcc tgaaaggaga
     4501 ttaatgagga agtgattacg tgcaacagat agcccgctct gtcgccctgg cgtttaataa
     4561 tttaccgcga ccacaccgcg ttatgttggg gtcgctcacc gttcttactc tggccgtcgc
     4621 tgtctggcgg ccctatgttt atcaccgcga tgccacgcca attgtcaaaa ccattgagct
     4681 ggaacagaac gaaattcgtt cgctcttacc tgaagccagt gagccgattg atcaagctgc
     4741 acaagaagat gaagccattc ctcaggacga actggatgac aaaatcgccg gtgaagcggg
     4801 cgtgcatgaa tatgttgttt ccactggcga tacgctaagc agcattctca atcagtatgg
     4861 tattgatatg ggtgatatca cccaactggc tgcggccgac aaagaattgc gtaacctgaa
     4921 aatcggtcaa caactctcct ggacattaac cgcggacggc gaactgcagc gcctcacctg
     4981 ggaagtgtct cgtcgtgaaa cccgaaccta tgaccgtact gccgctaacg gttttaaaat
     5041 gaccagcgaa atgcagcaag gagagtgggt taacaatctg ctgaaaggta ccgtcggggg
     5101 gagctttgtt gccagcgcca gaaacgccgg tttaaccagc gccgaagtga gcgcagtgat
     5161 taaagccatg cagtggcaaa tggatttccg caaactgaaa aaaggcgatg aatttgcggt
     5221 attaatgtct cgagaaatgc ttgatggtaa acgtgagcaa agccagctgc tgggcgtacg
     5281 tttgcgttca gaaggtaaag attattacgc tatccgcgct gaagatggca aattctacga
     5341 tcgtaacggt actggtctgg cgaaaggatt cttgcgattc ccgacggcga aacagttccg
     5401 tatctcgtct aactttaacc cgcgtcgtac taatccggtg accggtcgcg ttgcaccaca
     5461 cagaggtgtt gatttcgcca tgccacaagg tacgccagtg ctttcagtgg gtgacggtga
     5521 agtggtggtt gccaaacgca gtggtgcagc aggttattat gtagctattc gtcatggtcg
     5581 cagctacacc acgcgttata tgcacttgcg caagattctg gtgaaaccgg gacagaaggt
     5641 gaaacgtggc gaccgtatcg cgctttccgg taataccgga cgttcaaccg ggccgcatct
     5701 gcactatgaa gtatggataa accagcaggc cgtaaacccg ctgacggcaa aactgccgcg
     5761 taccgaaggg ctgaccggct ccgatcgtcg cgaattcctg gcacaggcca aagagattgt
     5821 gccgcagcta cggtttgatt aattaacatc cattcgcagc cggtacgcag tcagtaccgg
     5881 ctttttttat ttggtgcggg gcaagttgcg ccgctacact atcaccagat tgatttttgc
     5941 cttatccgaa actggaaaag catggaaacg aaaaaaaata atagcgaata cattcctgag
     6001 tttgataaat cctttcgcca cccgcgctac tggggagcat ggctgggcgt agcagcgatg
     6061 gcgggtatcg ctttaacgcc gccaaagttc cgtgatccca ttctggcacg gctgggacgt
     6121 tttgccggac gactgggaaa aagctcacgc cgtcgtgcgt taatcaatct gtcgctctgc
     6181 tttccagaac gtagtgaagc tgaacgcgaa gcgattgttg atgagatgtt tgccaccgcg
     6241 ccgcaagcga tggcaatgat ggctgagttg gcaatacgcg ggccggagaa aattcagccg
     6301 cgcgttgact ggcaagggct ggagatcatc gaagagatgc ggcgtaataa cgagaaagtt
     6361 atctttctgg tgccgcacgg ttgggccgtc gatattcctg ccatgctgat ggcctcgcaa
     6421 gggcagaaaa tggcagcgat gttccataat cagggcaacc cggtttttga ttatgtctgg
     6481 aacacggtgc gtcgtcgctt tggcggtcgt ctgcatgcga gaaatgacgg tattaaacca
     6541 ttcatccagt cggtacgtca ggggtactgg ggatattatt tacccgatca ggatcatggc
     6601 ccagagcaca gcgaatttgt ggatttcttt gccacctata aagcgacgtt gcccgcgatt
     6661 ggtcgtttga tgaaagtgtg ccgtgcgcgc gttgtaccgc tgtttccgat ttatgatggc
     6721 aagacgcatc gtctgacgat tcaggtgcgc ccaccgatgg atgatctgtt agaggcggat
     6781 gatcatacga ttgcgcggcg gatgaatgaa gaagtcgaga tttttgttgg tccgcgacca
     6841 gaacaataca cctggatact aaaattgctg aaaactcgca aaccgggcga aatccagccg
     6901 tataagcgca aagatcttta tcccatcaaa taaaaaacag cctctcaaat tattgagagg
     6961 ctgtacttga taaaaaatct caatgagtgt tctcaccatt ttgatttttt agattagtac
     7021 gctattgagt taatcgtagc gtgataattt caaaaggttt gaattcaaga ttgcaatatt
     7081 gattgcaata aaaactgtca tctaatttat tttcttggag atcgacatat tgaacgtagt
     7141 gataattaca tacccacgaa agcatggctg aa
//
LOCUS       DAOFQD010000190         7122 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.191, whole genome shotgun sequence.
ACCESSION   DAOFQD010000190 DAOFQD010000000
VERSION     DAOFQD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.191"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(374..4147)
                     /gene="rhsD"
                     /locus_tag="QBF46_004449"
                     /pseudo
     CDS             complement(374..4147)
                     /gene="rhsD"
                     /locus_tag="QBF46_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
     gene            complement(4215..4529)
                     /locus_tag="QBF46_004450"
     CDS             complement(4215..4529)
                     /locus_tag="QBF46_004450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676551.1"
                     /translation="MRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCG
                     KARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMR
                     EY"
     gene            complement(4539..6323)
                     /locus_tag="QBF46_004451"
     CDS             complement(4539..6323)
                     /locus_tag="QBF46_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDQ6676552.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIG"
BASE COUNT         1384 a         2190 c         1856 g         1692 t
ORIGIN      
        1 ataactcacc gagcacttgt aggcttatag gcgcatttgt attcatagag gggttgctaa
       61 acccgcacgc ctcaaaaatc atgttcgtcc ggctaaaaca attatttgca acaggaagag
      121 gcccgaggtt aatagttttt agttttttat taaggtctat tgccttattc atcatacatt
      181 gttcttcttc atctgttgta tctattatcc aaatccaggt atctctgcgg ggttgcatct
      241 ttttaaaata atcagagaca gaggctccca tctcatcccc agttcccgaa ctcattaatc
      301 catatcgatt tgccgccgca gcatgcccaa aaggattatc aggaacgggg ccgttaccta
      361 ttatcatagg actaagccct aatggatcaa cgtgttgcac tggattcagc ggatacgcat
      421 acagactcca tccccccgcc agcccaatcg ggtcctgggt gatataccgc ccctgcaacg
      481 gatcgtagta ccggtgacgg ttataatcca gccccgactc ctcatcgtac tgctgccctg
      541 gcagacggta tggctgatac aggtaatacg ggttctcttc attcagctgg ttgccccatt
      601 catcgtattc tgcactccac acagtattgc cgtcttcgct gatgagcgcc agcggcaggc
      661 cccggtggtc gcagtggtaa aaatgaactt ttcgcgccgg tgtgtattcc ggctccacct
      721 gtctggccag ttgctccacc gtcagcccgc actgcgcaag ccacacccgg ctttcactgc
      781 tcacgcggtc tgcccggatt tcttcctcca gtctgtccag cagccgcacc agttcagccg
      841 gaaataccac gccgtgcccg tcctcgctcc cttcctgctg cagcttctcc gccaggctgc
      901 ggcgctgcgc tttctcccgc tcaccgttgt ctgtttcgat gcggatgagc ggcgcgaagc
      961 tccccggctg atataccgtc tggatacgcg tggtgtcggt ctgtaccgtc gtcagccggt
     1021 cgccgtccca cccgtaccac gtcacctccg gtttacgcga cagcgacatc caccccgtca
     1081 ggtcacgctc ccgccgccat acccgtttcg ccattcgccg tcccagcggg tcgtaaaggt
     1141 agcggctctc gaccagtggc tcgccatgct gtatccgcgt gtagaacacc agacggtgct
     1201 ggctgtcgta gtggtagtgg tgggtccgct cgtcgtccgt ccggataaca cccgccggga
     1261 taaggtccgt cttctccgtc agtctgccgt attcatcata gtggtaaaca tagtgcgcat
     1321 cctctgcgat gcggttatcc ggccacgccg tgagggtact gtccgggtgc agctccgggt
     1381 ccggcaggcg gtttcccgcc gggtcggtgg catacgggat gcggatgtcc aggtccggtg
     1441 cgagggtacg cacgctctcc agcctgcccg tggcgctgta gccgtattcc cgcgtctgtc
     1501 gcgggccgct gatgcgcacc aggtcgccgt tgtcattcca cccgtagtca cggtcataca
     1561 ccaggctgtt caggtgctgg ctctgtaact ggcctgcggg ggtgtatgtg ctggtcagtt
     1621 tatatgcggc attactgcct gccatgctgc caaagctgcg caccgtctcc cggtgcatgc
     1681 ggtcgcgcgt gtactccagc agcggcgtgt cgccaagctt catgcccgcc agataaccgc
     1741 tgccgtaggt cagccactcc accggcggca ggctgtccgg cgtgacgcgg tttgccagcc
     1801 cctgttcgtt gtaggcgtgt gttgtctcat gctgccacag cagttccccc gtctccgggt
     1861 tctccaccgt ctgccgttcg ccggtcaggc ggcccttatc gtcgtagccg tagtggacag
     1921 cgacacggtg gccttcgctc aggtggctga tgtctgtcag ccagccgtgg tcatcatact
     1981 gccactgctc tgccggttcg ccgttcaccg tgcggtgagt gatacggtcc gattcatcgt
     2041 agtaccagag ggtgacaagc ccctcatctt cactctgcgt gagttttccg gtcaggtcat
     2101 aatgataacg ttgcgtccgc ccgtcaaagc cgccctgctg taccagccgg tccagcgcat
     2161 cgtagctgaa gtcgctgtgg ctgccgttct cgttagtcag actgatgaca cgtccggcag
     2221 catcgtactc catactgcgc gtcagcccgc cctgcgtggt gctgaccgcc tttccccacg
     2281 catcgtactg tgtctcgctc cggttgccgt ccggggtgat aacggcagtc aggtcgcctg
     2341 cggcgttgta ctcatactgc gtttcacggc cctgtgcgtc tttcaccgag gttaaccggc
     2401 cacggttgtc atagtggcgg taaaggctga taccttcctc gcggtgaacc gccgtcatct
     2461 ggccgaagcg gtcgtattca tagcgggtct ggtagcccga gcagtcagtg aacgccagca
     2521 actgcccgta gcggctccac gtcatctgcc gggtgctgcc cgtcgcatcc gttgtcgtcg
     2581 ccggtaactc actgtgcgcg tcatcgtagc ggtagcgtac tgtctccccg ctgcgcgatg
     2641 tctccgatac cagcctgccc ggttcatcat attcccggcg gctctccagc ccgtccgggg
     2701 acaccaccgc cgtcagctgg ttcccgtcgt tatagtaaaa tttcgtctcc cgcccgtccg
     2761 gcgtggtgat gtccgtgata tcgcctgaca ccacattcag accgtactct gtccgccgtc
     2821 ccgccgcgtc cgtctgcgcc gtgagccttc ctgccgcgtc atacccgctg tgcgtgacgc
     2881 tgccgtccgc cagttctttt ttcaccaccc gcttcagccc ggccccgcct tctgtatgca
     2941 gcacctcacg ccggttcagg ctgtccgtga cggtgatgcg atcctgctca tactggtagc
     3001 ggtaactcag gcctgccggg ttaagttgct ccaccacccg ccccgcatcg tcgtagcggt
     3061 agcgcatctc cggcctgcct gcataacggt gcgccaccat ccggcccggg tgctgcgcgt
     3121 catacgtgaa agcgcgcacc tgcgtattgc tgcggtcata taccgccagc agttcaccgg
     3181 cttccgtata tgtgtaccgc accagtggcg cagcgggcag gctctccggg tatgccgggt
     3241 cgtgcatcag ccacaccgcc gaaaggcgga tacccctgtc ggggccgtat tcggtaccgg
     3301 gcagtgtgtc ggggaacgct gaggctgaga gagggcggga actgtcagaa gaagatagcg
     3361 aagaggtgcg ggcctcttcc gcacgctgcg cctgcgtggt cagcaccaga cggaactccc
     3421 gcccggcacc gtccgtcacg ccggtgattt ccccggtcag gtcaccggcg gcctcacgcc
     3481 ggtacgtcag cgtccgcccg aagcggtccg ccagccccgt cagctcccgg tacggcggca
     3541 gcggcgctgg cagcacgtcc tcagcacccg gcacccgctc tgaccagccg agtatccacc
     3601 acggcccctg tgcgctgttg gtcgccaggt aaagatgcgg gcttaaccgg atatccggcg
     3661 gcagcgcccc ccacagccgc gccagcgtat ggccgtccgg ctgtgctgcc ttaccaccgc
     3721 gcaccagcca cattgactca ctgcggctgt acaccgcctc ccccggcagc agcggctcaa
     3781 agtgaatgct ccgcccgccg ttgtcgttga gtatcagtcc gtcatcacgt agctgtaagc
     3841 ggatatcaga aggcgctttc cagccggggc cgaaaacgcc caccggtgca ggcgtcttcg
     3901 tccggtagct gctgtaggtg cgggagagaa tgaacggcag cgggccgggc agcgcaaggt
     3961 ccgtctcgcc gggcagcacc ttcgccccaa gcagcggatt taccgggttg cccgaagtca
     4021 tcccgcccgg acacaccgag cacgccacgc cggtgggcgc gccaattctt acacctgccg
     4081 aaccctggac aatgggaccg ccatactgag tcatatctcc ctgacgcgcc gctggttttc
     4141 cgctcatgat tcaaattcct tcttctgttt atttttaatg ctggtttgtt actgaattcc
     4201 gaacaggtac gggatcagta ctctctcata tccggcgtat cctgcttttc atcaggggtc
     4261 ttcggtgggg atttcgaggc tgcacagttc cagttaatca gcagtgagtc accattaacc
     4321 tgctccttac cctgcaaatg aatttttgtg ccgttgagaa aaatttgtcc gtccttcgtc
     4381 agcaccagcc ttgccttacc acagcagagc tccagatgct caccggcgga gtaaatcgcg
     4441 gtctgcccgg tatcatcctt tttcgtttta ccaacggtga aggtaacgtt atttccgact
     4501 ttgacgtcat aacccagccc gaccctcaac gatttatgct aaccgatctg cgaggactgc
     4561 atcagtgcca ccgaggtgtt cataatgcca cctaccgtcg tctgatacgc cacgcccacg
     4621 gtcagcgcac gaacaagtcc aatcgtttcc acctgaacag aacccacttt aattatctgg
     4681 ttactgccca ccgtctctat ctggttaaca cccaccgtct gtatctgatt gttggtaacc
     4741 gcaatcatct gattgttgcc aatggtttca gcatggttgt ttatcacatc agtggtgcgg
     4801 ttattcagca cctcggtgtt catgttcttc tgcgcgtgga tgtagacctg ttctttccct
     4861 gtcgcatcgt caaacttcag ttcattaaac ccgctgccct tataggtttt cgaacgaatg
     4921 gtcatctgcg tcttcgtccc cggcaggctg ccgggtgtgc ggttttcctg gtggtaggta
     4981 cgccccataa taatcggctg gtccgggtcg ccgttgagga agtccacaat cacctcctga
     5041 cccactcgcg gtatcgccag gttgccaaag ccggtgcctg cccacgcctg tgccacacgg
     5101 atccagcatg aactgtcctg gtttgacggg ttatagcggt cccagttaaa tttcacccgc
     5161 acgcggccat gttcatcaca gaagatttcc tcgcctgccg gtcccgtcac gacggcgctc
     5221 tgcgggccgt ccaccagcgg tttcagcaac ggctgtggcc gccatgtccg gtctgccggt
     5281 attaccgcaa agtggttatt cagcgtggta cctgaaccac tgcgtcccgg caccgcctgc
     5341 ggctgctcgc cgtgcagttc acttgccacc acctgccatt cccggttcag gttcgcctgc
     5401 ggatgccccg tcagcgcaat tcgccgtccc ggccatatct ccggagaacg gcttgttccg
     5461 cgcgccactt ctgcgttgtt tcgccagcca tccatctgcc agcgggcaaa gttctgcccg
     5521 tgggcaccct tgaaacgtcc ggggtagtca tacacttcat actgtgtgcg ctggtaatcc
     5581 tggtactggc cttcctgatc aaaacgccct gcccagccgg gtcgtttaaa ggtgtagtcc
     5641 ttggtcacca cggaagaagg gcggatttgt gcgctgtagc ggaactggct gatgcagagg
     5701 gtgctcacct cggtacgggt gttcggattc caggggatct caaaggactc cggcagataa
     5761 cgcacggtgt cgcacaggac caggctctgg tcggtacttt tttgcgcgtg ctcctcataa
     5821 aagaagatgc cttcctccgc cgccatccgg cacaggaaat cgtaatcggt ttcgccgtac
     5881 tggacacaaa actcacggga aggatgcggc tcgctgaaca gcggactcca ctcggtcaca
     5941 ccgttttcct tcaggatcgt agcgaggatg ctttcgatgt cctcattctg gaagatacgg
     6001 aagttctggc gcagccctgt gcgccacagc ggcgggcaca ccttcatgct gtacagcatc
     6061 tggtttttgt cgttctcccc cagttcaaac caggtcacca cacctttcac ccggcgctgt
     6121 acgtcatcgc cctgccatac cgtcaggtag gccattttgt ccagcacctg cgcaaattca
     6181 agggaaagaa actgctgact gaccagagaa aggtcgaggg aaaaaagtga agagagtgac
     6241 tgggtgagat gaaaggaaac caccgcaaac gcgtccggtg gcaggccgtc cacttcaagt
     6301 gtgaaacgta atccggttga catagcaacc tccgtgaaaa tgaataataa tccgcacata
     6361 ttgcatgtac aacatgtcgg gcggatgaga taagcgagtc gacatctggc gttaccgggg
     6421 aatatcagaa aaatgtcatg gttatgtgat gatttagtct gggattttac ttcagagaaa
     6481 cgggtgtctg tttagtcaac aaaataatca caaacgcaac ggatgggttc tcgacgttat
     6541 tcacgacagg ctcgtacatt acagcgagta ataaaaaacc tgatatgcaa agtcaaacca
     6601 atattcataa tgtcttttta ttcacaacaa taaataattc aataacaatc attgaaatga
     6661 atgatgattt gtataaggaa tatacgctac cgccccaccc tgataacatg atctgcacat
     6721 tgccatctta acgtcgttaa acactggctt aatagtggat atttcaaatt aggaatttat
     6781 taaattcacc tgatgaatat cataagcaaa tagcatagaa aataatgaca attcccccga
     6841 ggggaccatt tccaaagcac tgcaccacca ccaggtgccc ggatgatctt gcttcagtgg
     6901 taagagtacc tgattcattg ttcccaaaat gatgttttcc ccggccccgc caataatgtg
     6961 cagctctcac gtgataatct attgattagg ttatattaag aatcaatatt taaaatacat
     7021 ccattttctt gaataaatca acatgtttat ttatcaattg ctgttttagc tctgactcat
     7081 caagctctcc atatttgtcc tacagataat tgcgaataaa ta
//
LOCUS       DAOFQD010000191         7121 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.192, whole genome shotgun sequence.
ACCESSION   DAOFQD010000191 DAOFQD010000000
VERSION     DAOFQD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.192"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..648
                     /locus_tag="QBF46_004452"
     CDS             <1..648
                     /locus_tag="QBF46_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDQ6676553.1"
                     /translation="VVQTNWMKKGVLDQCDKISSERVLVIKPDIKTFNNTKFDVDKDV
                     SVKTLLYPATPLTYKNHLVILKALVILKQKYFIDDLKFQVTFEKNRYKNFDKFVQLNN
                     LSKNIDYLGVLSYSKLQKKYMAASFIVFPSYIESYGLPLIEAASLGKKIISSDLPYAR
                     DVLKNYSGVDFVIYNDEDGWARALFNVLNGNSKLNFRPYEKDSRSSWPQFFSILK"
     gene            657..1691
                     /gene="fnlA"
                     /locus_tag="QBF46_004453"
     CDS             657..1691
                     /gene="fnlA"
                     /locus_tag="QBF46_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     4,6-dehydratase/5-epimerase"
                     /protein_id="HDQ6676554.1"
                     /translation="MFNGKILLITGGTGSFGNAVLRRFLDTDIKEIRIFSRDEKKQDD
                     MRKKYNNQKLKFYIGDVRDYSSILNASRGVDFIYHAAALKQVPSCEFHPMEAVKTNIL
                     GTENVLEAAIANRVRRIVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKSGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIQTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDM
                     GDYYRVPPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKKLLLKLPFIRAI
                     RSGEDYELDS"
     gene            1693..2799
                     /locus_tag="QBF46_004454"
     CDS             1693..2799
                     /locus_tag="QBF46_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HDQ6676555.1"
                     /translation="MKILVTGASGFIGRNLVFRLKEAGYNELITIDRNSSLADLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNSNVTQQIVDVLKKNNKNTPIMLSSSIQAECDNA
                     YGKSKATAEKIIQQYGETTNAKYYIYRLPNVFGKWCRPNYNSFIATFCHRIANDETIT
                     INDPSAVVDLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLITEDVGNGFTRALYSTWLSYLSPEQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIVIRGSACFKFENIVTGERYEFNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            2796..3926
                     /gene="wecB"
                     /locus_tag="QBF46_004455"
     CDS             2796..3926
                     /gene="wecB"
                     /locus_tag="QBF46_004455"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDQ6676556.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIIHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDDVLEQEKPEAMLVLGDTNSCI
                     SAIPAKRRKIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMPQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDVPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESERVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            3926..5134
                     /locus_tag="QBF46_004456"
     CDS             3926..5134
                     /locus_tag="QBF46_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDQ6676557.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDIT
                     LQAIYSVSMIDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWRALKHLIQHDTFDGI
                     VYYSPSIFWGDLVKKIKQRCQCPSYLVLRDMFPQWVIDAGMLKAGSPIEKYFRYFEKK
                     SYQQADRIGVMSDKNLEIFRQTNKGYPCEVLRNWASMTPVSASDDYHSLRQKYDLKDK
                     VIFFYGGNIGHAQDMANLMRLARNMMRYHDAHFLFIGQGDEVDLIKSLAAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLADSALRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            5125..5532
                     /locus_tag="QBF46_004457"
     CDS             5125..5532
                     /locus_tag="QBF46_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HDQ6676558.1"
                     /translation="MRLVDDNILDELFRTAVNSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEKNGEIQKQFIVGDGTGISVVEFSPGD
                     IHSVKCLSPNALMLEIKEGPFDPLKAKAFSKWL"
     gene            5645..7051
                     /gene="gndA"
                     /locus_tag="QBF46_004458"
     CDS             5645..7051
                     /gene="gndA"
                     /locus_tag="QBF46_004458"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDQ6676559.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         2072 a         1341 c         1589 g         2119 t
ORIGIN      
        1 gtagtgcaaa cgaattggat gaaaaaagga gtgttggatc aatgtgataa gattagttcc
       61 gaaagggtcc ttgttataaa acctgatatc aaaacattta ataatactaa atttgatgta
      121 gataaggatg tatctgttaa aacactctta tatccagcga caccacttac ctataaaaat
      181 catttggtca ttctgaaggc gttggttatt ttaaagcaaa agtattttat agatgatctg
      241 aaattccaag tgacttttga aaagaatagg tacaaaaatt ttgataagtt tgtgcaatta
      301 aataacctaa gcaaaaacat tgattatctc ggtgttcttt catactcgaa attgcaaaaa
      361 aaatatatgg cggcatcttt tattgttttt cctagctata tagaatcata tgggttacca
      421 ctcatcgaag ctgctagttt aggaaaaaaa atcattagta gtgatcttcc ttatgcccgt
      481 gatgttttaa agaattatag cggcgtagat tttgtaattt acaatgatga agatggctgg
      541 gctagggcgt tgtttaatgt tttaaatggc aattcgaagc tcaattttag gccttatgaa
      601 aaagatagtc gttcatcttg gccacagttc ttctctattt taaaataagg tgtattatgt
      661 ttaatggtaa aatattgtta attactggtg gtacggggtc tttcggtaat gctgttctaa
      721 gacgttttct tgatacagat atcaaagaaa tacgtatttt ttcccgggat gaaaaaaaac
      781 aagatgacat gaggaaaaaa tataataatc agaagcttaa gttctatata ggtgatgttc
      841 gcgattattc gagtattctc aatgcttctc gaggtgttga ttttatttat catgctgcag
      901 ccctgaagca agtgccttcc tgcgaattcc accccatgga agctgtaaaa acgaatattt
      961 taggtactga aaacgttcta gaagctgcaa tagctaatcg cgttaggcga attgtatgtc
     1021 tgagtacaga taaagccgta tatcctatta atgcaatggg catatctaaa gcaatgatgg
     1081 aaaaagtcat tgttgcaaaa tcacgtaatc ttgatagttc aaaaacagtt atctgcggaa
     1141 cacgttatgg gaatgtaatg gcttcacgtg gatcggtcat cccattgttt gttgatctaa
     1201 tcaaatctgg taaaccattg accattaccg atcccaatat gactcgtttc atgatgacgc
     1261 ttgaggatgc tgttgatctg gtcctttatg ccttcgagca tggaaataac ggtgatattt
     1321 tcgttcagaa agctcctgcg gcaacaattc aaacattagc cattgcactt aaggaattgc
     1381 taaatgccca tgaacatcca atcaatatta ttggaactcg acacggggaa aaactttacg
     1441 aagcgttatt gagccgagag gaaatgatag cagcggaaga tatgggtgat tattatcgtg
     1501 ttccaccaga tctccgcgat ttgaactatg gtaaatatgt ggaacatggt gaccgtcgta
     1561 tctcggaagt ggaagattat aactctcata atactgagag attagatgtt gagggtatga
     1621 aaaaattact gctaaaactt ccttttatcc gggcaattcg ttctggtgaa gattatgagt
     1681 tggattcata atatgaaaat tttagttact ggtgcttcag ggtttatcgg ccgtaatttg
     1741 gttttccgcc ttaaggaggc tggttataac gaacttatta cgatagatcg taactcttct
     1801 ttggcggatt tagagcaggg acttaagcag gcagatttca tttttcacct tgcaggagta
     1861 aatcgtcctg tgaaggagag tgaatttgaa gagggaaata gcaacgtaac tcaacagatt
     1921 gttgatgttc tgaaaaaaaa taataaaaat actcctatca tgctgagttc ttccatccag
     1981 gctgaatgtg ataacgctta tggaaagagt aaagcgactg cggagaaaat cattcagcag
     2041 tatggggaaa cgacaaatgc caaatattat atttatcgct tgccgaatgt attcggtaag
     2101 tggtgtcgcc caaattataa ctcctttata gcaactttct gccatcgcat tgcaaatgat
     2161 gaaactatta caattaatga tccttcagca gttgttgatc tggtgtatat agatgacttt
     2221 tgttctgaca tattaaagct attggaagga gcgaacgaaa ctggttacag gacattcggt
     2281 ccaatttatt ctgttactgt tggtgaagtg gcgcaattaa tttaccgatt taaagaaagt
     2341 cgccaaacat taatcaccga agatgtaggt aatggattta cacgtgcatt gtactcaaca
     2401 tggttaagtt atctgtctcc tgaacagttt gcgtatacgg ttccttctta tagtgatgat
     2461 agaggggtat tctgtgaagt attgaaaacg aaaaacgcgg gccagttttc gttctttact
     2521 gcgcatccag gaattactcg ggggggacat tatcatcatt ccaaaaatga gaaatttatt
     2581 gtcatccgag gaagtgcttg tttcaaattt gaaaatattg tcacgggtga gcgatatgaa
     2641 tttaatgtct cctcagatga ttttaaaatt gttgaaacag ttccggggtg gacgcatgac
     2701 atcactaata atggctcgga tgagctagtt gttatgcttt gggcaaatga aatatttaat
     2761 cgttctgaac cagatactat agcgagagtt ttatcgtgaa aaaattgaaa gtcatgtcgg
     2821 ttgttgggac tcgtccagaa attattcgac tctcgcgtgt ccttgcaaaa ttagatgaat
     2881 attgtgacca ccttattatt catactggcc aaaactacga ttatgaattg aatgaagttt
     2941 ttttcaaaga tttgggtgtt cgcaaaccag attattttct taatgccgca ggtaaaaatg
     3001 cagcagagac tattggacaa gttatcatta aagttgatga tgtccttgaa caggaaaaac
     3061 cagaagctat gttagttctt ggcgatacta actcctgtat ttcagcaata ccagcaaagc
     3121 gtcgaaaaat tccgatcttc catatggaag cggggaatcg ttgttttgac caacgcgtac
     3181 cggaagaaac taacagaaaa atagttgacc acactgctga tatcaatatg acatatagcg
     3241 atatcgctcg tgaatatctt ctggctgaag gtgtaccagc cgatagaatt attaaaaccg
     3301 gtagcccaat gtttgaagta ctcactcatt atatgccgca gattgatggt tccgatgtac
     3361 tttctcgcct gaatttaaca cctgggaatt tctttgtggt aagtgcccac agagaagaaa
     3421 atgttgatac ccctaaacag cttgcgaaac tggcgaatat acttaatacc gtggctgaaa
     3481 aatatgatgt cccggtagtt gtttctactc atcctcgcac tcgtaaccgc atcaatgaaa
     3541 acggtattca attccataaa aatatcttgc ttcttaagcc attaggattt cacgattaca
     3601 accatctgca aaaaaatgca cgtgctgttt tatcggatag tgggactatt acagaagagt
     3661 cctccattat gaacttccct gcactcaata tacgagaagc gcacgaacgc ccggaaggct
     3721 tcgaagaagg ggcagtaatg atggtcggtc ttgaatctga gcgcgtttta caggcattag
     3781 aaattattgc aacacagcct cgtggagaag tacgcttact tcgtcaggtc agtgactata
     3841 gcatgcccaa tgtttcagat aaagttgtgc gtattatcca ttcatatact gactacgtta
     3901 aacgggttgt ctggaaacaa tactaatgaa acttgcatta atcattgatg attatttgcc
     3961 ccatagcaca cgtgttgggg ctaaaatgtt tcatgagtta ggccttgaat tgttgagcag
     4021 aggccatgat gtaactgtaa ttacgcctga catcacatta caagcaatct attctgttag
     4081 tatgattgat ggtataaagg tttggcgttt caaaagtgga cctttaaagg atataggtaa
     4141 ggctaagcgt gccataaatg aaactctttt atcttttcgt gcatggcgtg cattaaagca
     4201 cctcattcaa catgatacat ttgatggtat cgtttattat tccccctcta ttttttgggg
     4261 agacttggtt aaaaaaataa aacagcgatg ccagtgccca agctacctgg tcctaaggga
     4321 tatgtttcca cagtgggtca ttgatgcagg tatgttgaaa gccggttcgc caattgaaaa
     4381 atatttcagg tattttgaaa aaaaatcata tcagcaggct gaccggatag gggtaatgtc
     4441 tgataagaat cttgagatat ttcgtcagac caataaaggt tatccgtgtg aagttttacg
     4501 taattgggcc tcaatgactc ctgtgtctgc cagcgatgat tatcattcac ttcgtcaaaa
     4561 atacgatcta aaagataaag tcattttttt ctatggcggt aatattgggc atgctcagga
     4621 tatggcgaac ttaatgcgcc ttgcgcgtaa tatgatgcgt tatcatgatg ctcatttcct
     4681 gtttataggg cagggtgatg aagttgacct gataaaatct cttgctgcag aatggaattt
     4741 aactaatttc actcacctac cttcagtgaa ccaggaagag tttaaattaa ttttatctga
     4801 agttgatgtc ggcctgttct ccctttcatc tcgccattct tcacataatt tccctgggaa
     4861 attactaggg tatatggttc aatcaatccc gatccttggg agtgtgaatg gcggcaatga
     4921 tttaatggat gtaattaata agcacagggc cggtttcatt catgttaatg gtgaagatga
     4981 taaactgttc gaatctgcac aattgcttct tgcagattcg gctttaagaa agcagttagg
     5041 tcagaacgct aatgtgttgt taaaatctca attttcggtt gaatcggcgg cacatactat
     5101 cgaagtccga ctggaggcag gagaatgcgt ttagttgatg acaatattct ggatgagctt
     5161 tttcgcacag cagtaaattc tgaacgtttg cgcgctcatt atttattgca cgcatctcat
     5221 caggagaagg ttcaacgttt acttattgca tttgtacgcg acagctatgt tgagccccat
     5281 tggcatgagt taccgcatca gtgggaaatg tttgtcgtca tgcaagggca attagaagtt
     5341 tgtttgtatg agaaaaatgg tgagatacaa aaacagttta ttgttggaga cggtactgga
     5401 ataagcgtcg tggaattttc cccaggtgat atacatagtg tcaaatgcct gtcaccgaat
     5461 gcccttatgt tggagataaa ggaaggacca tttgacccac tgaaagctaa ggctttttct
     5521 aagtggttat agggcgatac accacccttt attcttctat cttattctat acatgctggg
     5581 ttaccatctt agcttcttca agccgcgcaa ccccgcggtg aacaccccct gacaggagta
     5641 aacaatgtca aagcaacaga tcggcgtcgt cggtatggca gtgatggggc gcaaccttgc
     5701 gctcaacatc gaaagccgtg gttataccgt ctctattttc aaccgttccc gtgaaaagac
     5761 ggaagaagtg attgccgaaa atccaggcaa gaaactggtt ccttactata cggtgaaaga
     5821 gtttgttgaa tctctggaaa cgcctcgtcg catcctgtta atggtgaaag caggtgcagg
     5881 cacggatgct gctattgatt ctctcaagcc atacctcgat aaaggtgaca tcatcattga
     5941 tggtggtaac accttcttcc aggacacaat tcgtcgtaac cgtgagctgt ctgcagaagg
     6001 ttttaacttt atcggtaccg gtgtttccgg tggtgaagaa ggtgcgctga aaggaccttc
     6061 catcatgcct ggtgggcaga aagaagccta tgaactggtt gctccgatcc tgaccaaaat
     6121 cgccgccgtt gctgaagatg gcgaaccgtg cgttacctat attggtgccg atggcgcagg
     6181 tcactatgtg aagatggttc acaacggtat tgaatacggt gatatgcagc tgattgctga
     6241 agcctattct ctgcttaaag gtggcctgaa tctctctaac gaagaactgg cgcagacgtt
     6301 taccgagtgg aataacggtg aactaagcag ctacctgatc gacatcacca aagacatctt
     6361 cactaaaaaa gatgaagacg gtaactacct ggttgatgtg atcctggatg aagcggctaa
     6421 caaaggtacc ggtaaatgga ccagccagag cgcgctggat ctcggcgaac cgctgtcgct
     6481 gattaccgag tctgtgtttg cacgttatat ctcttctctg aaagagcagc gtgttgccgc
     6541 atctaaagtt ctctctggcc cgcaagcgca gccagctggc gacaaaggtg agttcatcga
     6601 aaaagttcgc cgtgcgctgt atcttggcaa aatcgtttct tacgctcagg gcttctctca
     6661 gctgcgtgca gcgtctgaag agtacaactg ggatctgaac tacggcgaaa tcgcgaagat
     6721 tttccgtgct ggttgcatca ttcgtgcgca gttcttgcag aaaatcaccg atgcttatgc
     6781 cgaaaatccg cagatcgcta acctgctgct ggctccgtac ttcaagcaaa ttgccgatga
     6841 ctaccagcag gcgctgcgtg atgtcgttgc ttatgcagta cagaacggta tcccggttcc
     6901 gaccttcgct gctgcggttg cctattacga tagctaccgt gccgctgttc tgcctgcgaa
     6961 cctgatccag gcacagcgtg actatttcgg tgcgcatact tataagcgca ttgataaaga
     7021 aggtgtgttc catactgaat ggctggatta atctgattta accaacaata aaattgaggc
     7081 ccggcgtata ttgcaccggg cttttttttg ccaattatct t
//
LOCUS       DAOFQD010000192         7043 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.193, whole genome shotgun sequence.
ACCESSION   DAOFQD010000192 DAOFQD010000000
VERSION     DAOFQD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.193"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..873
                     /gene="exuT"
                     /locus_tag="QBF46_004459"
     CDS             <1..873
                     /gene="exuT"
                     /locus_tag="QBF46_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDQ6676560.1"
                     /translation="SSIGAMIAPPLVVWAIVMHSWQMAFIISGALSFIWAMAWLIFYK
                     HPRDQKHLTDEERDYIINGQEAQHQVSTAKKMSVGQILRNRQFWGIALPRFLAEPAWG
                     TFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFADLGCILGGYLPPLFQRWFGVNLIVSR
                     KMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCIGGFAHQALSGALITLSSDVFGRNEVA
                     TANGLTGMSAWLASTLFALVVGALADTIGFSPLFAVLAVFDLLGALVIWTVLQNKPAI
                     EVAQETHNDPAPQH"
     gene            1003..1779
                     /gene="exuR"
                     /locus_tag="QBF46_004460"
     CDS             1003..1779
                     /gene="exuR"
                     /locus_tag="QBF46_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDQ6676561.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2124..2786
                     /locus_tag="QBF46_004461"
     CDS             2124..2786
                     /locus_tag="QBF46_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6676562.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2790..3173
                     /gene="mzrA"
                     /locus_tag="QBF46_004462"
     CDS             2790..3173
                     /gene="mzrA"
                     /locus_tag="QBF46_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDQ6676563.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3320..3688
                     /locus_tag="QBF46_004463"
     CDS             3320..3688
                     /locus_tag="QBF46_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDQ6676564.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADRQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3726..4031
                     /locus_tag="QBF46_004464"
     CDS             3726..4031
                     /locus_tag="QBF46_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDQ6676565.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4034..4438
                     /locus_tag="QBF46_004465"
     CDS             4034..4438
                     /locus_tag="QBF46_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDQ6676566.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4428..4727
                     /locus_tag="QBF46_004466"
     CDS             4428..4727
                     /locus_tag="QBF46_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDQ6676567.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4913..5305
                     /locus_tag="QBF46_004467"
     CDS             4913..5305
                     /locus_tag="QBF46_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDQ6676568.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5375..6361
                     /gene="yqjG"
                     /locus_tag="QBF46_004468"
     CDS             5375..6361
                     /gene="yqjG"
                     /locus_tag="QBF46_004468"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDQ6676569.1"
                     /translation="MGQLIDGIWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            6654..7019
                     /locus_tag="QBF46_004469"
     CDS             6654..7019
                     /locus_tag="QBF46_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDQ6676570.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1695 a         1735 c         1819 g         1794 t
ORIGIN      
        1 tcttcgattg gtgcgatgat tgcgccgccg ctggtggtat gggcaatcgt aatgcacagc
       61 tggcagatgg catttatcat ctccggtgca ttgagcttta tctgggcgat ggcatggctg
      121 attttctata aacatccgcg cgaccagaag catctgaccg atgaagaacg cgactatatt
      181 attaatggtc aggaagccca gcaccaggtg agcacggcga agaaaatgtc cgttggtcag
      241 atcctgcgta accgtcagtt ctggggtatc gcgctgccgc gtttcctggc agaaccggcc
      301 tggggtactt ttaacgcgtg gatcccgctg ttcatgttta aagtttacgg ctttaacctg
      361 aaagagatcg cgatgttcgc ctggatgccg atgctgtttg ctgacctcgg ttgtatcctc
      421 ggtggttacc tgccgccgct gttccagcgt tggtttggtg tgaacctgat cgtttcccgt
      481 aagatggtcg taacgctggg tgctgtgctg atgattggcc cgggtatgat cggtctgttc
      541 accaacccgt atgtcgcaat tatgctgctg tgtatcggtg gttttgccca ccaggcactg
      601 tctggcgcgc tgattacgct ctcttctgac gtgttcggtc gtaacgaagt ggcgacggcg
      661 aacggtttaa ccgggatgtc cgcatggctg gcaagtacgc tgtttgcgct ggttgtcggt
      721 gcactggctg acaccatcgg cttcagcccg ctgttcgcag ttctggcagt atttgacctg
      781 ctgggtgcgc tggttatctg gaccgtattg cagaacaaac cggcgattga ggtggcgcag
      841 gaaacacata acgatcccgc accgcaacat taacgtttcg tgtaagtttt agtttccctt
      901 caaagccgcc ttctcaggcg gctttttcat cactgcgagt agagctaaac tcgcaaaagt
      961 ggtataacaa atatagtctg ccgtatcatg cctggagcgc atatggaaat cactgaacca
     1021 cgccgtttgt atcaacaact tgccgctgac ctgaaagagc gcatcgaaca gggcgtctat
     1081 ctggtgggtg ataaactgcc tgccgagcgc tttattgccg atgaaaagaa cgtcagccgt
     1141 acggtagttc gtgaagccat catcatgctg gaagttgaag gctatgtcga agtgcgtaaa
     1201 ggttcaggta ttcacgtggt ttccaaccag ccgcgccatc agcaggctgc ggacaataat
     1261 atggaattcg ccaattacgg tccgtttgag ttactccagg ctcgccagct catcgaaagt
     1321 aatattgccg agttcgcggc aactcaggta acgaaacagg acatcatgaa attgatggcc
     1381 atccaggagc aggcacgcgg tgaacaatgc ttccgtgatt ccgagtggga tttgcagttc
     1441 catattcagg tagccctggc gacgcagaac tccgccctgg cggctatcgt tgaaaaaatg
     1501 tggacccagc gtagccataa cccgtactgg aaaaaactgc acgaacatat tgattcccgt
     1561 accgtcgata actggtgtga tgaccacgat caaatcctca aagcgctgat tcgcaaagat
     1621 cctcatgctg ctaagctggc aatgtggcag cacctggaaa acaccaagat catgttattt
     1681 aacgaaacca gcgacgactt cgagttcaat gccgaccgtt atctgttcgc cgaaaacccg
     1741 gtagtgcatc tcgatacggc caccagtggc agtaaatgaa atacctgtcc gacaggcgct
     1801 gcgacgcgcc tgtttgtctc gtcggtaagc gaaaagtata aagtgtcagc ctgtgtaaat
     1861 cctctcgcca ccctcccctg cattcagcaa aatcagactc cacggacatg taattttgat
     1921 aacgaacaac gttgaccttt gttacaatta gattcaattt gaatttatgt ttttgaatgc
     1981 tttcttatct cacgatttaa cagggaatag ttcaggctgt gttgatgtat caaacccgca
     2041 gaacatacca aaacagcaat aacattgcgg tagtgcatct tttaaaacca gcgtggcgtt
     2101 aaccgattca ccaggaataa tgaatggaac ttttgaccca attgctgcaa gccctgtggg
     2161 cgcaggattt tgaaaccctg gccaatccat cgatgattgg catgttgtat tttgtcttgt
     2221 ttgtaatttt gttccttgaa aacggcttgc ttccggcggc ctttttaccg ggcgacagtt
     2281 tactggtatt ggtcggcgtg ttgattgcga aaggcgcgat gggctatccg caaacgattc
     2341 tgctgctgac cgttgccgcc agcctcggct gctgggtcag ctatattcag gggcgatggc
     2401 tgggcaatac ccgcaccgta caaaactggc tatctcattt acccgcgcat tatcatcaac
     2461 gcgcacacca tctttttcat aaacacggtt tatcggcgct gttaattggt cgctttattg
     2521 cgtttgtcag aacactactg ccgacgattg ccgggttatc agggctgaat aacgcgcgct
     2581 ttcagttttt caactggatg agcggtctgc tgtgggtatt gatcctgaca actctgggtt
     2641 acatgctcgg caaaacgccg gtatttttaa agtacgagga ccagctgatg tcatgcctga
     2701 tgctgctccc ggtggtgctg ctggtttttg gcctggcagg ttctctggtc gtgttatgga
     2761 aaaagaaata tggaaatcgg gggtaaggga tgcaaatacc tcgcatgtcg cttcgccagc
     2821 tagcctggtc cggcgctgtt ttacttctgg tcggcacgct gttgctggcc tggtccgcgg
     2881 ttcgccagca agagtctacg ctggcgattc gtgccgttca tcaaggcaca acgatgccag
     2941 acggtttttc aatctggcat caccttgacg ctcatggcat tcctttcaaa agtatcaccc
     3001 ccaaaaacga cactctgtta attacctttg actccagcga ccagagcgcc gctgcaaaag
     3061 cggtcctcga cagaacattg ccccaaggct acatcattgc gcagcaggac aataacagtc
     3121 aggctatgca gtggctgacc cggctacggg ataactctca tcgcttcgga taacttcctg
     3181 ttatccgaaa cagatcactc actttggtga tttcaccgta actgtctatg attaatgagg
     3241 cggtggttaa cactaccgct tcgttgtctg gacaatcgtt cctttgtaat aggtccgaat
     3301 cacaatggaa ggttcaagaa tgaaataccg catcgcttta gctgtttctc tttttgctct
     3361 tagtgctggt agttatgcca ctaccctgtg tcaggaaaag gagcaaaata tccttaagga
     3421 gatcagctat gccgaaaaac accaaaacca gaatcgtatt gacggtctga ataaagccct
     3481 gagtgaagtc cgggccaact gttcagatag ccagctgcgt gccgatcgtc agaagaaaat
     3541 cgcaaagcag aaagatgagg tggcggaacg ccagcaagat ttagccgagg cgaagcaaaa
     3601 aggcgatgcc gataagattg ccaaacgcga acggaaactg gcagaagcgc aggaagagct
     3661 gaaaaagctg gaagcgcgcg actactaact cacaatagtc actacttact cacctggaga
     3721 aaactatgtc gaaagaacac actacggaac atctgcgtgc tgagttgaaa tccctttccg
     3781 atacgctgga agaggtgctt agctcatctg gcgagaagtc gaaagaagag ttgagtaaga
     3841 ttcgtagcaa agcggagcag gcactgaaac agagccgtta tcgcctgggt gaaaccggtg
     3901 atgccatcgc caaacaaacc cgtgtcgcgg cggcgcgtgc cgatgagtat gtgcgtgaaa
     3961 atccatggac gggcgtgggc attggcgctg caatcggtgt agtgctcggc gttctgctgt
     4021 cgcgtcgtta attatggcgg acactcatca cgcgcaaggg cccggtaaaa gcgttctggg
     4081 catcgggcag cgaattgttt ccatcatggt tgaaatggtg gaaacacgtc tgcggctggc
     4141 ggtggttgag ctggaagagg aaaaagctaa tctctttcaa cttttactga tgctgggcct
     4201 gacgatgctt ttcgctgcat ttggtcttat gagcctgatg gtgctaatta tttgggcggt
     4261 tgatccgcaa tatcgcctga atgcgatgat tgccaccacc gtggtgttgc tgctactggc
     4321 actgattggc ggtatctgga cgttacgtaa atcgcgtaaa tccaccttgc tgcgccatac
     4381 acgccatgag ctggcaaacg atcggcagct gctcgaggag gagtcccgtg agcagtaaag
     4441 tcgaacgtga acgacgtaag gcgcaactgc ttagccagat ccagcaacaa cggctggatc
     4501 tttctgccag tcgtcgtgaa tggctggagg caacaggcgc ttacgatcgt cgctggaata
     4561 tgctgctaag tctgcgctcc tgggcgctgg ttggcagtag cgtgatggcg atctggacga
     4621 ttcgccatcc taatatgctg gtccgctggg ccagacgcgg ttttggcgta tggagcgcct
     4681 ggcgtctggt taaaacgacc ctcaagcagc aacagcttcg cggttaactt ccctctggcc
     4741 ggagccattc cggccttacc catcaaattt tttgaagatt tttgacagtt ttccttgcta
     4801 acaatcatca ttcaccacgt ttatgattct ctccatcgac agcaacgacg ctaataccgc
     4861 gcccttgcac aaaaaaacaa tcagcagcct gagtggcgca gtggagagta tgatgaaaaa
     4921 attagaagat gttggtgtac tggtagcacg cattttaatg ccgattctgt ttattaccgc
     4981 tggctgggga aaaattactg gctacgcggg tacccaacaa tatatggaag caatgggcgt
     5041 cccgggtttt atgctgccgc tggtgattct gcttgagttc ggtggtggtc tggcaatcct
     5101 gttcggtttc ctgactcgca ccactgccct gtttactgcg ggctttacgc tgctgacggc
     5161 atttttattt cacagcaact ttgctgaagg cgtcaactcg ctgatgttca tgaaaaacct
     5221 gacaatttct ggcggattcc tgctgctggc aattaccggt ccgggcgcgt atagcatcga
     5281 ccgcctgctg aataaaaagt ggtaagcacg cgctatactt aacgataaaa aacgaggagg
     5341 aagctcctcg tttttgctat tggaggagag aaaaatgggt caactgattg acggcatctg
     5401 gcatgacacc tggtacgata ccaaatctac cggcggtaaa tttcaacgtt cagcttccgc
     5461 atttcgtaac tggctcactg ccgatggcgc tcctggcccc actggcacag gcggttttat
     5521 cgcagagaaa gatcgttatc atctctatgt ttcactcgcc tgcccgtggg cgcaccgcac
     5581 gctgatcatg cgcaaactca aaggactgga accgtttatt tccgtttccg tagtgaaccc
     5641 gctgatgctg gaaaacggct ggacctttga tgacagtttt ccgggagcaa ccggagacac
     5701 gctctatcaa catgaatttt tgtatcagct ttatctccac gccgatcccc actacagcgg
     5761 acgagttact gttcccgtgc tgtgggacaa aaagaaccac accatcgtca gcaacgaatc
     5821 agcagaaatc atccgcatgt ttaataccgc gtttgatgcg ctgggcgcga aagcgggtga
     5881 ttactaccca ccagccctgc aaacgaaaat tgacgaactt aacggctgga tttatgacac
     5941 cgttaacaac ggcgtgtata aagctggttt tgccaccagc cagcaagcct acgacgaagc
     6001 ggtggagaaa gtgtttgaat cgctggcgcg actggaacag attttaggtc agcatcgtta
     6061 cctgaccggc aaccagctaa ccgaagccga tattcgcctg tggaccacgc tggtccgttt
     6121 tgatccagtg tatgtgaccc acttcaagtg tgataagcac cgcatcagcg attaccttaa
     6181 tctgtatggc ttcctgcgcg atatctacca gatgccggga attgccgaaa cagtcaattt
     6241 cgatcacatc cgtaatcatt acttccgcag ccataagacc atcaacccta cggggattat
     6301 ttcaattggt ccgtggcagg atctcgatga accgcatgga cgagatgttc gcttcggtta
     6361 aaaaaaggca ccttacgagg tgcctctgat aattagctaa gtgcatgttg ttcatgtcgg
     6421 atgcggcgtg aacgccttat caaacctaca aaacatttat attcaatatg ttgcaatgac
     6481 cgcgcaggcc agataagcgt agcgcatctg gcgattttgc gcttgtcatc agtcttagca
     6541 cgccttcata gccctttatt aattccttga acgaatattt actgccattt cattcacgtc
     6601 tattcttaat ttgctgcttt aaaaacaagt gattgagcaa aattgaggca aaaatggact
     6661 ggtatctgaa agtactaaaa aattatgtcg gtttccgggg tcgtgcgcgg cgcaaagagt
     6721 actggatgtt tattctggtc aacatcatct ttacgttcgt gctggggctg ctggataaaa
     6781 tgttaggctg gcaacgcgct ggtggcgaag gcatcctgac gacaatctac ggtattctgg
     6841 tgtttttacc gtggtgggca gttcagttcc gtcgcctgca cgacaccgac cgctcggcgt
     6901 ggtgggcact gctattctta atcccgttta tcggctggct tattatcatc gtctttaact
     6961 gccaggcggg tacgccaggc gagaaccgct ttggaccaga tccaaaactg gaaccttaaa
     7021 acaaatctgc tcgccggatg tct
//
LOCUS       DAOFQD010000193         6846 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.194, whole genome shotgun sequence.
ACCESSION   DAOFQD010000193 DAOFQD010000000
VERSION     DAOFQD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.194"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..147)
                     /gene="cas6e"
                     /locus_tag="QBF46_004470"
     CDS             complement(<1..147)
                     /gene="cas6e"
                     /locus_tag="QBF46_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDQ6676571.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRR"
     gene            complement(129..875)
                     /gene="cas5e"
                     /locus_tag="QBF46_004471"
     CDS             complement(129..875)
                     /gene="cas5e"
                     /locus_tag="QBF46_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDQ6676572.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVVPTPEAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(886..1941)
                     /gene="cas7e"
                     /locus_tag="QBF46_004472"
     CDS             complement(886..1941)
                     /gene="cas7e"
                     /locus_tag="QBF46_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDQ6676573.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKKEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1953..2489)
                     /gene="casB"
                     /locus_tag="QBF46_004473"
     CDS             complement(1953..2489)
                     /gene="casB"
                     /locus_tag="QBF46_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDQ6676574.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2486..4048)
                     /gene="casA"
                     /locus_tag="QBF46_004474"
     CDS             complement(2486..4048)
                     /gene="casA"
                     /locus_tag="QBF46_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDQ6676575.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLDKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4146..6845)
                     /gene="cas3"
                     /locus_tag="QBF46_004475"
                     /pseudo
     CDS             complement(4146..6845)
                     /gene="cas3"
                     /locus_tag="QBF46_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT         1657 a         1861 c         1620 g         1708 t
ORIGIN      
        1 ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca gccactgatg
       61 catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa gctggccggt
      121 atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg cccctgattg
      181 acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg gcggcgtaat
      241 attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc ctgaagtttc
      301 gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc attacataag
      361 gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag cggatgactt
      421 tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa ctgttcgagg
      481 ctgtaaggtg cttcgggggt tggtaccacc gccaccatcc accaggcgtc ggtataatag
      541 tcgcgtcggg agataattgc gctcaaaaga tcagggtcgc tcaactcttc gcgacggctg
      601 aaataacgcg ctttacgcac ctcttttggc atctggactg tgtgataatc ccgtgcccag
      661 cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc gtttaagcgt
      721 tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa tgctgagcgc
      781 gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca ggacgccatc
      841 ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg cgcagataaa
      901 gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg cgcattcggt
      961 cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct gcaccgccac
     1021 atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag agcgtggttg
     1081 atcggtgcct ttttctgcca gcgcccagga ggcgtaggca cggctggcaa agctgttctg
     1141 tttgccggtt ggggagactt taagtgcggc ttccgtaaag gcacgtatgg tctgattagc
     1201 taacgcttcg tcaccgccaa ggttttcgac cagcagatct ttatcaatgc agatataggt
     1261 gtagaacagt gcagaaccga atccggtttc accaagatgc ccggcaccgg catcttcaga
     1321 agcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg tttcactgac
     1381 accaaatgcg tgcgctacct ggcaggcggc ttcaacatta aactcgggtt tattcgccag
     1441 catacgacca aacatggcaa tatctaccgc catacggtct ttgcgtaaca acgcgagatc
     1501 ttcctttttt ggcgcgcgct tttcttctgc aagctgatgg gccagcgctt ttacggcgtc
     1561 aaattctgcc gggctgatat ggactaactg ttcagtttcg gcgttagtga gcggatcttt
     1621 tggttttttg tcgtttttag ctttcccaag ataatctgca atttttgccg cccattcgat
     1681 ggcttttttc tcttcgattc ctttctcaat caggatagtt gccgcctcac gcgcaatacg
     1741 cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag aagtgcgcca
     1801 cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca gcaccacagt
     1861 ttttggcgca ccggtatcat cacggttaag gttggcagca gggtaagcgg ttaacaaatg
     1921 aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt atcggcgtcg
     1981 ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg ccgctgctgg
     2041 cggcggtgat gattgagcag gtcgtcgctc tcctggcacc aacggaagac cccctctgcc
     2101 agagagtcaa gattgacgga accgtttaat aatctgactg cgcggcgtaa ctggcgcagt
     2161 aattcatccg gtgtttttac tgccgacaga cgagtaaaac gcccctttga cattaccgcc
     2221 gccagctgcg cggcaaaagg ctgccgttca tcaatagctt taacattggc gctaactgcg
     2281 gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt tatttgtctg
     2341 ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt cgtactacgc
     2401 cgcagactgg cacgttcgcc acgtttctcc tgcaattcct cgtgccattt gcgcagcgtg
     2461 gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc tccctggcgg
     2521 ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg gtcatgacgc
     2581 gttccagatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa tcctgacgtg
     2641 cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc tgaccttctt
     2701 caatttggcg cactaacctc agaaagcgat gctgagtttt gttccagaag tcgatatcca
     2761 caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct ttcaatgcac
     2821 tccgtaacag acttagaatc cgtgaagctg tttgcgcagc cagccgcagc ttcggtatct
     2881 gaccttcttt tttatcgagc agcagcggga aatggtgttc gtaccaacag cgcgctttca
     2941 tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg agactgctgg
     3001 cattgaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgat ttacccgttt
     3061 cgataaggcc taaccagtca cgccagatta aaccgcccgg ttgtggttta acggagtaaa
     3121 actcgccacc ctctttaagt ggtacacggt aaggcgttaa gggatgctgc cacatggcat
     3181 aattcgcacc gtaatttttg gtcgtcatca ggctcaggag cgcgtcgctc tgctcaccgc
     3241 aaatatcgca gttgccgact gtcgtggtat taaaatcaat acgaatacgg cgaggcattc
     3301 cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg gccagttcgc
     3361 tggtgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg ggtagcggta
     3421 aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag ggagtttgtt
     3481 gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg ccgcgtaaac
     3541 cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac agagctaacg
     3601 cagaacaatg tgggcacagg tattccgtta caccacgctt gataaagtgg tctttattaa
     3661 acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga actttatctc
     3721 cggtgagcgc ctcgaaatcc tgcataaatg aaggggaatc tggcccaaac tggaaagcat
     3781 gttctaatga ctgcaatgct tcccgtagct tttcagcttc cagtccgtct tcccagatat
     3841 catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc agcaaaaact
     3901 gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca ttttcatccg
     3961 ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg ggcaaccacg
     4021 gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga ccatcccagg
     4081 tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt cctgtcgcag
     4141 acatctcaac caaccttgcc aatcaatcct tccgtagccg aatatccgca actttcgtca
     4201 tcgttgacta aaatcacgtt tgccatctcc ggatcttgtc gctgttcaat gcaccacagc
     4261 ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca ccagcttcgg
     4321 cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg cttcaccaca
     4381 ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg tgtcgacagt
     4441 ttttccggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt cagcacaaac
     4501 tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc gctgcgggca
     4561 aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag ccgggctgcg
     4621 tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca gattcgccca
     4681 tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc gtcgccagga
     4741 gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc gtcctttccg
     4801 tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc tgctcgctga
     4861 atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaat atccacgctt
     4921 tgctcaatta cttgtgtaca aacaatgact tgcgacgctc tctgtaaact acaatattta
     4981 ccaaagcgtg ccagcgtttc cgtttcaatt cgctggcgat cgctaaaagc aaagcggcta
     5041 tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg acgatgaacc
     5101 ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg acttaccgcc
     5161 gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct gacgctacgc
     5221 tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt gacatgcgtc
     5281 agccaggggt aatcatcctt ttccaggaac ggagcttctt gctggccctc tgtaccccgc
     5341 gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga aagcaaaata
     5401 acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca cgacatgtaa
     5461 gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc cagaagccgc
     5521 agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt gccgacgcca
     5581 atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca tccctgactg
     5641 aacgtttgtt catccggttc ttctgacccg actaaatcac cggaccagag tgattcatta
     5701 aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg ggattctggc
     5761 gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc cgttgccatt
     5821 gttggcagac caaaaaacag cccctgagct ttccccgcag ctatcaacct gtgcgccagg
     5881 ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa taactgcggt
     5941 ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg attctcaata
     6001 aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag aggaaagacg
     6061 gttaaggcgt tttgagcctg aattaaagtt ttctgccagt aacctttaat atccattggg
     6121 tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag cacgacggtt
     6181 gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc atcccagaat
     6241 gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc tcgtgctgca
     6301 gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg ccgaccatga
     6361 tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg cggagagagc
     6421 gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa ccagtagccc
     6481 agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat cttgagttca
     6541 gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg ccagcacagc
     6601 aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg acgaaaataa
     6661 tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc tacatccagc
     6721 gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg tcaggttttt
     6781 ccccagaaat caaaaaaagt cacaatattt ttatccttca gtaaacttaa aggatattta
     6841 cgcata
//
LOCUS       DAOFQD010000194         6792 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.195, whole genome shotgun sequence.
ACCESSION   DAOFQD010000194 DAOFQD010000000
VERSION     DAOFQD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.195"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(283..852)
                     /locus_tag="QBF46_004476"
                     /pseudo
     CDS             complement(283..852)
                     /locus_tag="QBF46_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(848..1542)
                     /locus_tag="QBF46_004477"
                     /pseudo
     CDS             complement(848..1542)
                     /locus_tag="QBF46_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019082309.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
     gene            1974..2837
                     /locus_tag="QBF46_004478"
     CDS             1974..2837
                     /locus_tag="QBF46_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDQ6676576.1"
                     /translation="MNNSEGLKSFQQSLAGLPQWVSERILQQINQLTHYEPVIGIMGK
                     SGAGKSSLCNALFASEVSPVSDVAACTREPLRFRLQVGDCYMTLMDLPGVGESGTRDT
                     EYAALYREQLSRLDLVLWLIKADDRALAVDEHFYHQVIGEAYRHKVLFVISQSDKAEP
                     TSGGGPLSTAQKQNISRKICLLHELFQPVHPVCAVSVRLQWGLRVMAERMIKCLPREA
                     TSPVVSQLQPSFRTTVVREQARSDFGETVGALLDSISAFPLIPTPVRAVIQAVRTTVV
                     SVARAVWDFFF"
     gene            2929..3750
                     /locus_tag="QBF46_004479"
     CDS             2929..3750
                     /locus_tag="QBF46_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDQ6676577.1"
                     /translation="MTRLASRFGAANLIRRDRPLTREELFRVVPSVFSEDKHESRSER
                     YTYIPTISLLDSLQREGFQPFFACQTRVRDPGRREHTKHMLRLRREGQITGIQVPEII
                     LLNSHDRTSSYQMLPGLFRAVCQNGLVCGESFGEVRVPHKGDVVSQVIEGAYEVLGIF
                     DRVEEKRDAMQSLLLPPPAQQALAKAALTYRFGEDHQPVTESQILSPRRWQDESNDLW
                     TTYQRIQENLIKGGLSGRNAKGGRSHTRAVRGIDGDVKLNRALWVMAEALLTQLQ"
     gene            3967..4668
                     /locus_tag="QBF46_004480"
     CDS             3967..4668
                     /locus_tag="QBF46_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDQ6676578.1"
                     /translation="MTQAERRHDRLAVRLSLIISRLVAGETLSVRKLAAEFGVSVRTL
                     RRDFRERLMYLDLEYQSGYCRLRTAGSETQMVPDVLIFAHRSGMAGLFPGFDRRLVNA
                     LLMCDESPCVIAPASPAPSPSGALSFWRLIQAITGRRRVTLIAEGQRCERLAPCRLLI
                     HQQAWYLVAEHDGHIAVFSLDEIHLVQPLQETFRRNDSLCRLVEDPGFIQSLPHFRFI
                     QQSLLTFVPADSPPE"
     gene            4709..4945
                     /locus_tag="QBF46_004481"
     CDS             4709..4945
                     /locus_tag="QBF46_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676579.1"
                     /translation="MPVIAIIAIVVIVIILNKTGVSDSLAALTLATVAALLTGGGAAG
                     AASVALTPFVGVPVGIFVGIYVFAKVVRLISGKK"
     gene            4945..5388
                     /locus_tag="QBF46_004482"
     CDS             4945..5388
                     /locus_tag="QBF46_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676580.1"
                     /translation="MKCKTLPLLAMVATTLFLSACDDRSDDLKAISKFKDLTPPRFSD
                     VVSRQDDVSEEWSQVGFSSGLTLQVLRTRESPDGCEGGSYYYLVDMQEKTIQPLMNAL
                     CIADNIKLEYQEVTDPYTKEKYFEYAHDGKLMGQLLIPSNPDNQE"
     gene            5411..5878
                     /locus_tag="QBF46_004483"
     CDS             5411..5878
                     /locus_tag="QBF46_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676581.1"
                     /translation="MTILSLSRFMLAGVLLASFNASAIPGFWQQGYGQGNTEYSVTEA
                     SGKTFTINCTGNPDQNGFYQHSVFLTLADDKMVSSHDDDTTITVVMDHQQYIIPSSLG
                     WRNGDNAWFDFISNISEAGQFDVYVNDHKAGTFTADRKNAEKVLSTLGDCSND"
     gene            5955..6194
                     /locus_tag="QBF46_004484"
     CDS             5955..6194
                     /locus_tag="QBF46_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HDQ6676582.1"
                     /translation="MTQSVLLPPGPFTRRQAQAVTTTYSNITLEDDQGSHFRLVVRDT
                     EGRMVWRAWNFEPDAGEGLNRYIRTSGIRTDTATR"
     gene            6292..6750
                     /locus_tag="QBF46_004485"
     CDS             6292..6750
                     /locus_tag="QBF46_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDQ6676583.1"
                     /translation="MTTQTQHDLAPANQPEFELTVTPVPDEQRIDFWPQYFGAIPQWL
                     LLEPHIFAWMDRFCEGYSGGIWSFYTLSNGGAFMSPEPDNDETWRLFNCLNGNDAQMS
                     AEAAGIAVCLIAYSHHACRTECDAMTAHYYRLREYAMQHPEAHAILRIID"
BASE COUNT         1488 a         1884 c         1839 g         1581 t
ORIGIN      
        1 agtggagcgg gccggcaaga ctcgcgagat gcttgtggaa ctacaaccgg gagtttgatg
       61 gaaagtgggt catggatctg cacagcgaag cagccaagcg acttcagcct tgcgccgaac
      121 tacgagccct agctagggat ggtctgaagt agccctgtat ttccggctga cttcagccac
      181 tgctgatttt gaaagcggtg aaccgatcaa aaacgatcgg atcacccgct catttcggtg
      241 ctttcagccg tcgcgagcac cttgcgacgg ccattttccg cattacaggc gcacctctcc
      301 tgcgcttgtc agcaaatgtc ggcgggccat ccataggttc gacagggcaa acagcgtcac
      361 cagttgtgca gtgttcctca ccagccctcg gaaacgtacc ttggtgtagc cgaactgccg
      421 cttgatcacg cgaaacgggg gctctacctt cgctcgcact tgcgccttgg ccttctcgat
      481 cttgcgcttg gctttgtaca gggcgctgcg tttatcgagc atcttgtagg tgctgcggcg
      541 tgctgcgacc tgccagatca cttctcggtc ggcatgctcg gagcgctttt cgacgccggt
      601 gtagccggca tcggcgcaga cgacgttctc gtcgccgtgc agcagcttgt cgacctgggt
      661 gacatccgcc acggtggctg ccgtgcccac cacgctgtgt accagccccg attcatcatc
      721 aacgccgatg tgcgccttca tgccaaagta atactggttc cccttcttgg tctggtgcat
      781 tttcgggtcg cgcttgccgt cctggttctt ggatgaactg ggcgcgtgga tcagcgtggc
      841 atcgacgata gtccatcttg ataatatcgg gccgctcaac gctcgggtac ggcacgaaat
      901 ccagctcgac gatgcccgtc cactggatgt cgttatcctg tcgttcgttg gcttgtggct
      961 cctcgtcgag caatgttggt gcttcgatgc gggtgacgac gaaatcccga aactctctac
     1021 tcttgcgatc aaagggacgt gccagcgcat gccggtatca accaaagcaa agggcacaat
     1081 tacccgctcg gactcgccgc tgctcatcga gtagtagagg atggcgacgg gtcgcttagc
     1141 gtggatgcct cggcaaatcg tggctaacac atccatcttg ggaatgctta gggctgcggg
     1201 ggattcgcat ggtagcagcg cctgcattga gccgttcacg ccatcgccaa aaccgagaga
     1261 cagcgccgac agaacgcgca gcgatgcgtg ctcgaacaac ggtgagaact cctgcccgat
     1321 gcgataaatc ttgttgctac catcgaaggt gatgttatgc ggcgcaattt cccggtacaa
     1381 agccaaatcg cgtgtcgccc ctgccggagc cacaccgaag cgctcaatca gatcagggcg
     1441 tccgatctca ccgaagaaat agagccgaaa atcgatgtag gccaacctct cgcgctggac
     1501 caaactcaag tcctcaagcc gcttagaaag ggaaccaagc atgaatcatc actataggga
     1561 aaaaatgaat atttaatacg gtaatccata aagtgccatg attataatca aattgatgat
     1621 aatcagcatt aatacttatg taacagcaag aatttatgtc ttaaacgtag acagtcatcg
     1681 tatgggctga tgtttctata ccgttaaatt gatgccatcc aactataaaa ccttgctgcc
     1741 caatgcccta tagctccctc aggcaatttg cccttatcca ctgttctggt ctggggatga
     1801 gggatatcag aataacctaa cgactcaggg actgggatca caggcaaccg tggctcgtcg
     1861 cttaccttaa ttcaaattat tttcagatag atatcattct tctgaacata cccgattaac
     1921 ggcagaatct cacgattctg ccgtttttgt ataatttttc agaggagtag ctaatgaaca
     1981 attctgaagg tttgaagtcg tttcagcaat cgcttgctgg cctgccacag tgggtgtctg
     2041 aacgtatatt gcagcagata aatcagttaa cccattacga gccagtaatc ggcattatgg
     2101 gtaagagcgg tgccggtaaa tcctcactct gcaatgccct gtttgccagt gaggtatcac
     2161 cggtcagcga tgtcgcggcc tgtacacgtg agccactgcg ctttcgactg caggttggcg
     2221 actgctatat gacgctgatg gatctacccg gcgtgggcga aagtggcacg cgcgataccg
     2281 agtatgctgc gctgtaccga gagcagcttt ctcgcctcga cctggtactg tggctgatta
     2341 aggctgatga ccgggcactg gcagtggatg agcactttta tcatcaggtg attggggagg
     2401 cataccggca taaagtgctg tttgttataa gccagtcgga caaggccgaa cccaccagcg
     2461 gtgggggacc gttgtccacc gcgcagaagc agaatatcag ccgcaaaatc tgcctgctgc
     2521 atgagctgtt ccagcccgta catccggtgt gtgctgtgtc ggtccgtcta caatgggggc
     2581 ttagggtgat ggccgagcgg atgattaagt gcctgccacg tgaggccacc agcccggtgg
     2641 tgtcgcaact ccagccttcc tttcgcacga cggtagtccg ggaacaggct cgaagcgatt
     2701 ttggtgaaac cgtcggtgct ctactcgata gcatcagtgc ctttcctctg atacccacac
     2761 ctgtgcgggc cgtcattcag gccgtgcgca ccaccgtggt ctctgtggcc cgcgccgtct
     2821 gggatttctt cttctgagta tttaatccat ccctgttatt tccctgaccc tgtcgccgat
     2881 gcgacgggga tcccctttat ttgttttccc gttatgagga gtctgcttat gacccgtctg
     2941 gcttcgcgct ttggcgcagc aaaccttatc cgtcgcgacc gtccgttaac ccgtgaagag
     3001 ctgtttcgcg tagtgcccag tgtgttcagt gaggacaaac acgagtcccg tagtgagcgt
     3061 tatacctata tacccaccat ctccctgctc gacagcctac agcgagaagg cttccagcca
     3121 ttctttgcct gtcagacccg cgtgcgtgac ccgggtcgtc gtgaacatac aaagcatatg
     3181 ctgcgtctgc ggcgggaagg gcagatcacc ggtatacagg tgccggaaat tattctactc
     3241 aactctcacg atagaaccag ttcgtatcag atgttgccgg gactatttcg tgcggtttgt
     3301 cagaacgggc tcgtctgcgg tgagtcgttt ggcgaggtgc gggtgccaca caagggggac
     3361 gtggtgagtc aggtgattga aggcgcgtat gaggtgctgg ggatttttga ccgggtggag
     3421 gaaaaacggg atgccatgca gtcgttgctg ttgccacccc cggcacagca ggcactggca
     3481 aaagccgccc tcacataccg ctttggtgaa gaccaccagc cggtgactga atcgcagatc
     3541 ctctcccctc gccgctggca ggatgagagc aatgacctgt ggaccacgta ccagcgtatt
     3601 caggagaacc tgattaaggg cgggctcagt ggccgtaatg ccaaaggagg acggtcacat
     3661 acccgtgccg ttcgcggtat cgacggggac gtgaaactta accgggcact gtgggtgatg
     3721 gcggaagccc tgctcacgca actgcagtga ccgtttaatg ttgctgcttt gttaatatcg
     3781 gacaccacct gtccgcatcg caccgtgctg acggtcctca ctcaccgagg gaaggcctgt
     3841 aacccccgct gcctctggct tttacagaaa tgaaaaatag tttctgtggt gtccataccc
     3901 tgtccgaccc cctctttaaa gtaatcacat cattttcagt cagttaactt tcacggagaa
     3961 cgtctcatga cacaggcaga acgccgccat gaccggctgg ctgtcaggct gtcactgata
     4021 atcagccgtc tggtggcagg ggaaacgctg agtgtgcgta agcttgccgc tgagtttggt
     4081 gtgtcggtgc gcacgctgcg gcgtgatttt cgtgagcggc tgatgtatct ggacctggag
     4141 tatcagtccg gatattgccg cttacgcact gcgggcagtg agacgcagat ggtgcccgac
     4201 gtgcttatct ttgcccaccg cagcgggatg gccggtcttt tccctggctt tgaccgtcgt
     4261 ctggtaaacg cactgctgat gtgcgatgag tctccctgcg taatcgcacc cgccagtccg
     4321 gctccttcgc catcaggcgc attgtctttc tggcgactga ttcaggccat taccgggcgc
     4381 aggcgggtga ctctgattgc cgaggggcag cgctgtgagc ggctggctcc ctgccggtta
     4441 cttatccacc agcaggcctg gtatctggtg gcagagcacg acggacatat cgccgtattc
     4501 tccctggatg aaatccatct ggttcagccc ctgcaggaga ctttccggcg taacgacagt
     4561 ctgtgtcgtc ttgttgaaga cccgggcttt attcagtcct taccccattt tcgctttatc
     4621 cagcagtcac tgcttacgtt tgttccggcc gacagcccac cagaatagcg taagcgttgt
     4681 tatcaacccg gcaacaggga ggagccctat gcccgttatt gccattatcg ccattgttgt
     4741 catcgtcatc attctgaaca aaaccggggt gtccgacagt ctcgcggccc tgacccttgc
     4801 cactgttgcc gcactgctga cgggaggtgg cgcagccggt gctgccagtg tcgcgctgac
     4861 accgttcgtc ggcgtgccgg tgggtatttt cgtgggcatt tatgtctttg ccaaagtggt
     4921 acgtctgatt tcagggaaaa aataatgaaa tgtaaaacac tacctctgct ggcgatggtt
     4981 gccaccactc tgtttctgag cgcctgcgat gacaggagtg atgacctgaa agctatcagt
     5041 aaatttaagg acctcacgcc tccgcgcttc agcgatgtgg tcagtcggca ggatgatgtc
     5101 agcgaagaat ggtcacaggt tggcttttca tcaggtctca ccctgcaggt cttacgtacc
     5161 cgtgagtcgc ccgatggctg cgagggcggt agttactact acctcgtgga tatgcaggaa
     5221 aaaaccatcc aaccgctgat gaatgcgctg tgtattgccg ataacatcaa actggaatac
     5281 caggaggtga cggacccgta taccaaagaa aaatactttg agtacgccca tgacggcaaa
     5341 ctgatggggc agctactgat accctcgaac cctgacaatc aggaataaaa caacgacaaa
     5401 ggagacaaga atgacaatac tttcactaag tcggtttatg ctggcgggtg tactgctcgc
     5461 gagctttaat gcctctgcta tcccggggtt ctggcagcag ggttacggtc agggcaatac
     5521 ggaatacagc gtgaccgaag ccagcggaaa gacgtttacc atcaactgca cagggaaccc
     5581 ggaccagaat ggtttctatc agcattcagt ctttcttacc cttgccgatg acaagatggt
     5641 cagttcgcac gatgacgaca ctactatcac cgtagtgatg gatcaccagc agtacattat
     5701 tccgtccagc ctgggctggc gtaacggcga taacgcctgg tttgacttca tcagcaatat
     5761 ctctgaggcc gggcagttcg acgtctacgt caatgaccac aaagcaggga ccttcactgc
     5821 ggaccggaag aacgctgaga aagttctgtc cactctcgga gactgcagca acgactgata
     5881 gtagtatctt ccccagcaaa tccaccccga cagctagcag gctgccgggg ttttctttta
     5941 tcaggagccc gaaaatgacc caatccgtgt tgctgccacc ggggcctttc acccggagac
     6001 aagcgcaagc ggtcactacc acgtacagca atatcacact cgaagacgac cagggcagtc
     6061 acttccgtct ggtggttcgt gatactgaag gccggatggt ctggcgggca tggaactttg
     6121 agccggatgc cggtgaaggt cttaaccgct atatccgcac ctcaggcatc cgtacagaca
     6181 cggccacccg ctgatcgcga agcatttacc cgcattcacc tccccgaaca cactttatat
     6241 ccccatacgc cagccatcgc cgctggcgtt tttattgacg gagacatacc catgacaaca
     6301 cagacgcagc acgacctcgc acccgctaac caacccgaat ttgaactgac cgtcacgccg
     6361 gtccccgatg aacagcgtat cgatttctgg ccacagtact ttggcgctat cccgcagtgg
     6421 ctactcctgg agccgcatat cttcgcctgg atggaccgct tctgtgaggg ctacagcggt
     6481 ggtatctggt cgttctacac cctcagcaat ggcggcgcat ttatgtcccc cgagcctgac
     6541 aacgatgaga catggcgtct gtttaactgc ctgaacggta acgatgccca aatgagtgca
     6601 gaagcagcag gtattgctgt ctgcctgatt gcgtatagcc accatgcctg ccgtacagaa
     6661 tgtgacgcga tgaccgcaca ctattaccgc ctgcgggagt atgccatgca acatccagag
     6721 gctcacgcca ttctgcgtat tatcgactga ccgaaggagc aacagatgaa acagctttcc
     6781 tttttacccg gc
//
LOCUS       DAOFQD010000195         6710 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.196, whole genome shotgun sequence.
ACCESSION   DAOFQD010000195 DAOFQD010000000
VERSION     DAOFQD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.196"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..752
                     /locus_tag="QBF46_004486"
     CDS             <1..752
                     /locus_tag="QBF46_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDQ6676584.1"
                     /translation="LGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNCN
                     GNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPITV
                     SSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEA
                     LLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAG
                     PEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1118..2185
                     /locus_tag="QBF46_004487"
     CDS             1118..2185
                     /locus_tag="QBF46_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ6676585.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2182..4917
                     /gene="rbbA"
                     /locus_tag="QBF46_004488"
     CDS             2182..4917
                     /gene="rbbA"
                     /locus_tag="QBF46_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDQ6676586.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEALPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLADLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4917..6041
                     /locus_tag="QBF46_004489"
     CDS             4917..6041
                     /locus_tag="QBF46_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6676587.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKRIGTMA"
     gene            6114..6389
                     /locus_tag="QBF46_004490"
     CDS             6114..6389
                     /locus_tag="QBF46_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDQ6676588.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6386..>6710
                     /locus_tag="QBF46_004491"
     CDS             6386..>6710
                     /locus_tag="QBF46_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDQ6676589.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYI"
BASE COUNT         1584 a         1658 c         1894 g         1574 t
ORIGIN      
        1 ttctgggcga ccagactggc gatcgattta gtaatatctg tggcgatcaa tttatcatcg
       61 atttattaaa acgcataatg agtatcaatg aaaacgttca tgtattagct ggaaatcatg
      121 aaacgaattg caatggtaac tatatgcaga acttcacgcg catgaaacca cttgatgagg
      181 atacctactc tgggataaaa gattatcctg tatgtttcta tgaccccaaa tacaaaataa
      241 tggcaaatca tcatgggata acttttgatg atcagcgaaa acgttacatt ataggaccga
      301 taacagtctc tagtgatgaa atgaccaatg ctctcgatcc ggtcgaatta gcggcaatta
      361 tcaataaaaa gcaccatgct gtaattaatg gcaaaaagtt taaaaatagc cgggctatct
      421 cttgtcgatc ctttaatcgt tatttttctg tttcgacaga ttacagacct aaacttgaag
      481 cactattagc atgctcacaa atgttaggta taaatcaagt tgttgcacat aacggtaatg
      541 gtggaagaga atgtataggt gaaacgggga cagttttagg tcttaacgcc cgtgacagta
      601 aacatgctgg aagaatgttc agcatgcata attgccaaat taaccctggt gcagggcctg
      661 agataaccac tccctggaag tcttaccagc atgaaaaaaa cagaaatgga cttatgcctt
      721 tgattagaag gcgcacaatg ttacagctct gaaccataga caacatgaat attaatgtta
      781 ctatctggtt gagactgttg ataaaacgta aaaaggaatg ctctgttcct gcaactgaac
      841 aggcgtgaaa cgcgatccaa caggatggat accgttttgt cagccagttt tatagatcac
      901 acttatgagt atgcgtgttt catttacggc caggaatgct gatgatgaca acattactac
      961 ccgttttcac taagccttcc ccgttggcgc tcaatgctct gcgcgctggt cgtatttgtc
     1021 gtttccttct tatcccggat ggaagaatcc gctgaacgtc gttcggcggc agttgtaaca
     1081 cgcgtcgttt gcttacaggg attatggcgg gccgattatg gataagagta agcgccatct
     1141 ggcgtggtgg gttgtcgggg cactggcggt ggctgccgtc gtggcgtggt ggctgttgcg
     1201 cccggcgggt gtgccggaag gctttgctgt cagcaatggg cgcattgaag cgacggaagt
     1261 ggatatcgcc agcaaaattg ccgggcgtat cgacaccatt ctggtgaaag aaggccagtt
     1321 tgttcgcgaa ggtgaagtgc tggcgaagat ggatactcgc gtgttgcagg aacagcgact
     1381 ggaagccatc gcgcaaatca aagaggcaca aagcgccgtt gccgccgcgc aggctttgct
     1441 ggagcaacga caaagcgaaa ctcgcgccgc gcagtcgctg gttaatcaac gccaggcaga
     1501 actggactcc gtagcaaaac gtcatacgcg ttcccgttca ctggcccaac gaggggctat
     1561 ttctgcgcaa cagctggatg acgaccgcgc cgccgctgag agcgcccgag ctgcgctgga
     1621 atcagcgaaa gcccaggttt cggcttctaa agcggctata gaagcagcac gcaccaatat
     1681 cattcaggcc caaactcggg tcgaagcagc gcaagccact gaacggcgca ttgccgcaga
     1741 tatcgatgac agcgaactga aagccccgcg tgacggacgc gtgcagtatc gggttgccga
     1801 gccaggggaa gtgctggcgg caggcggtcg ggtgctgaat atggtcgatc tcagcgacgt
     1861 ctatatgacc ttcttcctgc caaccgaaca ggcgggcacg ctgaaactgg gtggtgaagc
     1921 ccggctgatc ctcgatgccg cgccagatct gcgtattccg gcgaccatca gttttgtcgc
     1981 cagcgtcgcc cagttcacgc caaaaaccgt cgaaaccagc gatgaacggc tgaaactgat
     2041 gttccgcgtc aaagcgcgta tcccaccgga attactccag cagcatctgg aatatgtcaa
     2101 aacgggcttg ccgggcgtag cgtgggtgcg ggtgaatgaa gaacttccgt ggcctgacga
     2161 cctggtggtg aggttgccgc aatgacgcat ctggaactgg ttcccgtccc gcctgtcgcg
     2221 caactggcgg gcgtgagcca gcattatgga aaaaccgttg cgctgaacaa tatcactctc
     2281 gatattccgg cccgctgtat ggtcgggctg attggcccgg acggcgtcgg gaagtcgagt
     2341 ttgttgtcgt tgatttccgg tgctcgtgtc attgagcagg gcaatgtgat ggtgctgggc
     2401 ggcgatatgc gcgacccgaa gcatcgccgc gacgtctgcc cgcgcatcgc ctggatgccg
     2461 caggggctgg gcaaaaacct ctaccacacc ttgtcggtgt atgaaaacgt cgattttttc
     2521 gcccgcctgt tcggtcacga caaagcggag cgggaagtgc gaattaatga gctgctgacc
     2581 agcacggggt tagcaccgtt tcgcgatcgt ccggcaggta aactctccgg cgggatgaag
     2641 caaaaactgg ggctgtgctg tgcgttaatc cacgacccgg aactgttgat tcttgatgag
     2701 ccaacaacgg gggttgaccc gctctcccga gcccagttct gggatctgat cgacagtatt
     2761 cgccagcggc agagcaatat gagcgtgctg gtcgccaccg cctatatgga agaggccgaa
     2821 cgcttcgact ggctggtagc gatgaatgcc ggagaagtgc tggcaactgg cagcgccgaa
     2881 gagctgcggc agcaaacgca aagtgccacg ctggaagagg cgtttataaa tctgttaccg
     2941 caagcgcaac gccaggcgca tcaggcggta gtgatcccac catatcaacc tgaaaacgca
     3001 gagattgcca tcgaagcgcg cgatctgacc atgcgttttg gttccttcgt tgccgttgat
     3061 cacgtcaatt tccgcattcc acgcggggag atttttggtt ttcttggttc gaacggctgc
     3121 ggtaaatcca ccaccatgaa aatgcttacc ggactgctgc ccgccagcga aggtgaggcg
     3181 tggctatttg ggcaaccggt tgatccaaaa gatatcgata cccgccgtcg ggtaggctat
     3241 atgtcgcagg cgttttcgct ctataacgaa ctcaccgtgc ggcaaaacct ggagttacat
     3301 gcccgtttgt ttcacatccc ggaagcggaa attcccgcgc gagtggctga aatgagcgag
     3361 cgttttaaac tcaacgacgt tgaagatatt ctgccggagt cattgccgct cggcattcgc
     3421 cagcggcttt cgctggcggt ggcggtgatt catcgcccgg agatgttaat cctcgatgaa
     3481 cctacttctg gcgtcgatcc ggtggcgagg gatatgttct ggcagttgat ggtcgatctc
     3541 tcgcgccagg acaaagtgac tatcttcatc tccacccact ttatgaacga agcggaacgt
     3601 tgcgaccgta tctcactgat gcacgccgga aaagtgctcg ccagcggtac accgcaggaa
     3661 ctggttgaga aacgcggagc cgccagtctg gaagaggcat ttatcgccta tttgcaggaa
     3721 gcggcagggc agagcaacga agccgaagcg ctgccggtga tacacgacac cacccacgcg
     3781 ccgcgtcagg gatttagcct gcgccgtctg tttagctaca gccgccgcga agcactggaa
     3841 ttgcgacgcg atccggtccg ttcgacgctg gcgttgatgg gaacggtgat cctgatgctg
     3901 ataatgggtt acggcatcag tatggatgtg gaaaacctgc gctttgcggt gctcgaccgc
     3961 gaccagaccg tcagtagcca ggcgtggacg ctcaatctct ccggttcccg ttactttatc
     4021 gaacagccgc cgctcaccag ttatgacgag cttgatcgtc ggatgcgtgc gggcgatatc
     4081 acggtggcga ttgagatccc tcccaatttc gggcgcgata ttgcgcgtgg tacgcctgtg
     4141 gaactcggcg tctggatcga cggtgcgatg ccgagccgtg ccgaaacggt aaaaggttac
     4201 gtgcaggcca tgcaccagag ctggctacag gatgtggcga gccgacaatc cacacccgcc
     4261 agccaaagcg ggctgatgaa tattgagacg cgctatcgct ataacccgga cgtaaaaagc
     4321 ctgccagcga ttgttccggc ggtgatcccg cttctgctga tgatgatccc gtcaatgcta
     4381 agcgccctta gcgtggtgcg ggaaaaagag ttggggtcga ttatcaacct ttacgtgact
     4441 cccaccaccc gtagcgaatt tttgcttggc aaacagctac cgtacatcgc gctggggatg
     4501 ctgaactttt tcctgctctg cggcctgtcg gtgtttgtgt ttggcgtacc gcataaaggc
     4561 agtttcctga cgctcaccct ggcggcgctg ctgtatatca tcattgccac cggaatgggg
     4621 ctgctgatct ccacctttat gaaaagccag attgccgcca ttttcggaac ggcgattatc
     4681 acgttgatcc cggcgacaca gttttccgga atgatcgatc cggtagcttc gctggaaggt
     4741 ccaggacgtt ggatcggcga ggtttacccg accagtcatt ttctgactat cgcccgcggg
     4801 acgttctcga aagcgctgga tctggctgat ttgtggcaac tttttatccc gttgctgata
     4861 gccatcccgc tggtgatggg cttaagcatc ctgctgctga aaaaacagga gggatgatgc
     4921 gccatttacg caatattttt aatctgggta tcaaagagtt gcgcagtctg ctcggtgata
     4981 aagcgatgct gacgctgatt gtcttctcgt ttacggtgtc ggtgtattcg tcagcgaccg
     5041 ttacgccagg atcgttgaac ctggcaccga tcgccattgc cgatatggat caatcgcagt
     5101 tatcgaaccg gatcgttaac agcttctatc gcccgtggtt tttgccaccg gagatgatca
     5161 ccgccgatga gatggatgcc ggactggacg ccggacgcta taccttcgcg ataaatattc
     5221 cgcctaattt tcagcgtgat gtcctcgctg gacgccagcc agatattcag gtgaacgtcg
     5281 atgccacgcg catgagccag gcatttaccg gcaacgggta tatccagaat attatcaacg
     5341 gtgaagtgaa cagctttgtc gcgcgctacc gtgataatag cgaaccgttg gtatcgctgg
     5401 aaacccggat gcgctttaac ccgaacctcg atcccgcgtg gtttggcggg gtgatggcga
     5461 tcatcaacaa cattaccatg ctggcgattg tattgaccgg atcggcgctg atccgcgagc
     5521 gtgaacacgg cacggtggaa cacttactgg tgatgccgat aacgccgttt gagatcatga
     5581 tggcgaagat ctggtcgatg gggctggtgg tgctggtggt gtcgggatta tcgctggtgc
     5641 tgatggtgaa aggtgtgctg ggcgtaccga ttgaaggctc gatcccgctg tttatgctgg
     5701 gcgtggcgct cagtctgttt gccaccacgt caatcggcat ttttatgggg acgatagcgc
     5761 gttcaatgcc gcaactgggg ctgctggtga ttctggtgct gctgccgctg caaatgcttt
     5821 ccggtggatc cacgccgcgc gaaagtatgc cgcagatggt gcaggacatt atgctgacca
     5881 tgccgacgac acactttgtt agcctcgcgc aggccatcct ctaccggggt gccggattcg
     5941 aaatcgtctg gccgcagttt ctgacgctga tggcaattgg cggcgcattt ttcaccatcg
     6001 cactgctgcg attcaggaag aggattggga caatggcgta aggaagggaa acttgacttc
     6061 atactgtgat gtcatactat tgatgtcata aagtgaagtc ataaggacat gccatgagaa
     6121 ccaaggtaca ggctttacgg aagaaacaaa aaaatacatt ggatcaaata tttaaaactc
     6181 ctgttcctca agggattaag tggtctgata tagagtcact ggttaaagca ttaggcggag
     6241 aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc gttgcgtgtt
     6301 tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc gtgcgtgact
     6361 ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact ctatggagat
     6421 tgccgggcaa cctgcagtta tcacttacgt gccagaactg aatgcatttc gtggcaaatt
     6481 cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc tgcaaaaaga
     6541 aggggagctt tccctgcgag agtatcttga ggattgtaaa gctgcgggta ttgagccgta
     6601 tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc tgagtgaacg
     6661 ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta
//
LOCUS       DAOFQD010000196         6554 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.197, whole genome shotgun sequence.
ACCESSION   DAOFQD010000196 DAOFQD010000000
VERSION     DAOFQD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.197"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            457..1125
                     /locus_tag="QBF46_004492"
     CDS             457..1125
                     /locus_tag="QBF46_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676590.1"
                     /translation="MAASRITHLITSCTKGKHSQCGSMPELSIRSGQTPEEAMSSWAA
                     TIKRSQSASPVPALSLYAGNHWSTAKEILRTTENLELWVISAGLGFLNSRDLVDAYEA
                     TFHDLPFSHRQWWRELTNTFGKERTAKSIETLMAARPFDDYVIAASPVYIEATEDDIL
                     AGASKLNNHIAQLTVVTSGEYSGLLESYLIRSESRMMRQLSSNMVCLNIKLAQYIIGS
                     RRYS"
     gene            complement(1348..4356)
                     /locus_tag="QBF46_004493"
     CDS             complement(1348..4356)
                     /locus_tag="QBF46_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDQ6676591.1"
                     /translation="MAADFLTDKQTQNYGRYAAEPNEIQLARYFHLDERDLTFINLRR
                     GRHNRLGIALQLTTARFLGTFLPDLMQIPPGVQFYVARQLNIRYPEIISRYAQRENTR
                     WEHHGLIRQHYSYHDFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLL
                     PAASTLTRVIGEIRERATRRLWRKLAALPNRWQAAQLAGLLEIPEGQRLSVMEHLKRG
                     PVTISGPAFTEALERYTRLRSLEFSCLNFTGLPAIQLRNLARYAGMASVKYISRMPEE
                     RRLAILTAFVKAQEISALDEAVDVLDMLILNITREAKKTGQKKRLRTLKDLDRAALLL
                     ARACALLLDEDTGDDLLRKTIFSSVPVARLAESVEKVNELARPQDTNFQDEMVEQYGR
                     VRRFLPALLRDLHFRAAPDGEHTLAAIHYLAELNGSKKRILDDAPEHIISGPWKRLVY
                     DAEGRIQRAGYSLCLLERLQDALRRRDIWLENSDRWGDPRQKLLQGEEWQAQRVPVCR
                     ALGHPTNGSKASVQLAARLDETWKTVASRFDRNTAVDICNEGKHPSLTISSLDKLDEP
                     PALIRLSSRVRQLLPPVDLTELLLEIDARTGFTREFSHVSESGARAQDLHISLCAVML
                     AEACNIGHEPLIKHNIPALTRHRLSWVKQNYIRAETLVSANARLVDFQSSLALAGYWG
                     GGEVASADGMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVIPGTLRD
                     SIFVLEGLLEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRAD
                     KAANYGALDELARGCVDLSKIESHWDEMMRVAGSLKLGTIHASELIRSLLRSTRPSGL
                     AQAIMEVGRVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRY
                     REGQEDQLGALGLVTNAVVLWNTLYMQEALSHLRSTGEGPEDEHIARLSPLMHGHINM
                     LGHYTFTLPEDIMKGELRPLNLNLNNELPP"
     gene            4515..5096
                     /locus_tag="QBF46_004494"
     CDS             4515..5096
                     /locus_tag="QBF46_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6676592.1"
                     /translation="MALLGYARVSTSHQKLTSQITELNTAGVRDDRVFTDMMSGATDE
                     REGLQRLLARAEKDDIILCTKMDRLGRNTADMIRIVDTCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRQAAMIKGVKFGRKPHKKAELANELINKDVQ
                     VKEVMEKTGISRATYFRLKRKTVTNNIHLSNNK"
     gene            5173..>6554
                     /locus_tag="QBF46_004495"
     CDS             5173..>6554
                     /locus_tag="QBF46_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDQ6676593.1"
                     /translation="MSQYLKLGDDQSPVQLDPHSEVGAFKVVGHCAWAKPEDKITPDF
                     LRSRVEPWLTALFQSEHLNILIGAGLSSAIQESATGTKPQGMGWIEDLKVCKAEIDSY
                     VAKTADASGRGKGNIEDQIRSINELIKGLEILTVQNLPVPLPPPGAPPYRNLKSELVE
                     LNNELTRCLKLFSDSVSHGEKLIRDASNDLKTETFNYLVSFLMSFSSRTATRDRLHIF
                     TTNYDRIIETGAEIAGLRLIDRFVGTIAPIFRSSRLEVDYHYNPPGIRGEPRYLEGVA
                     RFTKLHGSLDWYATEGAIRRFGLPFGASSVEPFLQVETAGTANYQQLMIYPNSVKDRE
                     TSEYPYVELFRDLASATCRPNSTLVTYGYSFGDEHINRLIIDMLTIPSTHIVIIAYGD
                     PLGRIMRFINDSGRKAQISLLLGDHFGDLKKLVDFYLPKAAIDRSSIRMAELLKSRGL
                     YRATKPNNSE"
BASE COUNT         1789 a         1510 c         1562 g         1693 t
ORIGIN      
        1 ccacctctct agttaaaaaa attctcgtag cgtcatttat agtaacgcct ttactaatga
       61 ctaaagatga aaaagattag cacacagatg tcgggtgacc aacaacccgc aacccgttaa
      121 gtggcgatcg cattccctga atgcacaacc attgagctat atgactgatt aataaggttt
      181 ttatccgcaa ttacactatg aggggggcta tgtgtacgat gggatttacc cataattaaa
      241 tcccatagta cataaaatga actaaataaa gccgaaaaac gtgacggtgt cacctctggg
      301 gtgtttactt tggtatctag ttattagtaa aggcatgact aaatgtataa tggggtagtc
      361 gtttacgttt agggcttcct ctttgtcgtt atggcggatg aggggctgtt acgatagtta
      421 gacagtgatt acacacattt ggctgagggc tacaaaatgg cggcgtcgcg aattacacac
      481 ctaataacgt cctgtaccaa aggtaagcac agccagtgcg gctccatgcc agaactgagt
      541 atccggtctg gacagacacc agaagaggca atgtccagct gggcagcaac gattaaacgg
      601 tcgcaaagtg cttcaccggt accggccctg tcgctctatg ccggtaatca ctggtcaacg
      661 gcaaaagaga tattacggac aactgagaac ctggagttgt gggtgatctc ggccgggttg
      721 ggttttttga atagtcgtga cctcgtggac gcttatgaag ccacctttca tgatttaccg
      781 ttcagtcacc gacaatggtg gcgggaatta acaaatacat tcgggaaaga aaggacggcg
      841 aaatctattg agacattgat ggcagcaaga cctttcgatg attatgttat cgctgcctcc
      901 cccgtttata tcgaggcgac ggaggatgat atcctggcag gggcaagcaa attaaataat
      961 cacattgccc agttgacggt tgtaacgtca ggggaatatt ctggactact tgaatcctat
     1021 ttaattcgta gcgaatcccg tatgatgcgc caactatcaa gcaacatggt atgtcttaac
     1081 atcaaacttg ctcaatatat tattggaagt cggcgctatt catgaaccgc actgatgatt
     1141 ccataacgca actatctaca ctttatgaac ggctcggagc tgtggctcat gactggaata
     1201 ttgtaaaatt ctgctccctg aacactctga acaactggat cttgatgccg cagcaacata
     1261 tgcaatgatg ctccgcattg aacagtgggc agcaaacgtt aagaacgcat ataaggggtt
     1321 tgagggccaa tgatgtaaaa aaccacgcta aggaggtaat tcattgttta aattgagatt
     1381 taacggcctc aattccccct tcataatatc ctccggtagt gtgaacgtat aatgtcccag
     1441 catgttgata tgaccatgca tcagaggcga aagccgcgcg atatgttcat cttctggccc
     1501 ttccccagtg ctacgcaggt gcgacaaggc ttcctgcata taaagcgtat tccacagcac
     1561 cactgcgttt gtcaccagac ccagcgcacc caactgatct tcctgcccct cgcggtaacg
     1621 ttttctgatc tcaccacgct gaccgtagca aatggctctt gccacggcat ggcgaccttc
     1681 gccccggtta agctgtgtta ggatcctgcg gcgatagtcc tcatcatcaa tatagttgag
     1741 aagatacagt gtcttgttaa ctcttccgac ctccattatt gcctgagcaa gccctgacgg
     1801 tcgggtactt ctcaatagcg atcgaatgag ttctgacgca tgaattgtgc ccagcttcag
     1861 cgaacctgca acccgcatca tctcatccca gtggctttcg attttagaaa ggtcaacaca
     1921 accccgagcc agttcgtcca acgccccgta attcgctgct ttgtcagccc gccagaacac
     1981 cgcctcacca gcatccgcca gacgaggtga aaattggtat cccagtagcc agaacaagcc
     2041 aaagataatg tcactggtac ctgctgtgtc cgtcatgatt tcaaccgggt ttagtcccgt
     2101 ttgttgctca agtagtccct ccagcacaaa aatggaatcc cgcagcgttc cagggatcac
     2161 tatgccatga aaacccgaat attggtcaga gacaaagttg taccaggtaa tgccgcgccc
     2221 tgaaccaaag tattttctgt tggggccaga attcaccgtt ttcactggtg tgacaaaacg
     2281 cataccatca gcggaggcca cctcaccgcc accccagtat cctgccaacg ctaacgagga
     2341 ctgaaaatca accagccgcg cattggcgct gaccagcgtt tctgctcgga tgtagttctg
     2401 tttcacccag ctcaggcgat ggcgggtcag tgcagggata ttgtgcttga tcagcggctc
     2461 atgtccgata ttgcaggcct cagccagcat caccgcacac aggcttatat gcagatcctg
     2521 cgcgcgggca cctgactcgc tgacatggct gaattcacgg gtgaaacccg ttctggcgtc
     2581 aatctcaagc aacagttctg tcaggtcaac gggtgggagc aattgtctga ctcggctgct
     2641 cagtcgtatc agcgccggtg gctcatccaa tttatccagg ctgctgatgg tcagcgaagg
     2701 atgtttacct tcattgcaga tgtctacggc ggtattgcgg tcaaaacgtg atgccaccgt
     2761 tttccaggtt tcatccaacc gtgctgccaa ttgcaccgat gctttactgc cattggtggg
     2821 gtgacccaat gcccggcaca ctggaacccg ctgcgcctgc cattcctccc cctgaagaag
     2881 tttttggcgc ggatcacccc aacgatcgct gttttccaac cagatgtcac gacggcgtag
     2941 cgcatcctga agtcgctcca gcagacacaa cgagtaaccc gctcgctgta tccgaccttc
     3001 agcatcgtac accaggcgtt tccaagggcc tgaaatgata tgctcaggtg catcgtccag
     3061 gatccgtttt ttcgagccgt ttagctctgc caggtaatgg atggccgcga gggtatgttc
     3121 accgtctggg gccgcccgga agtgcagatc gcgcagtagt gcgggcaaaa aacgccttac
     3181 ccgtccgtac tgctccacca tttcatcctg aaagtttgta tcctgggggc gggccagttc
     3241 attcactttt tccactgact ctgccagtct ggcgacaggt acactgctga atatggtctt
     3301 tcgcagcaaa tcatcaccag tatcttcatc aagtaaaagt gcacatgccc gcgccagcaa
     3361 caacgccgca cggtcaagat ccttgagcgt cctaagtcgc tttttctgcc cggttttctt
     3421 cgcctccctg gtaatattga ggatcagcat atccagcacg tcaacggcct cgtccagcgc
     3481 cgagatttct tgcgctttca cgaacgcggt aaggatagcc agccgccgtt cttctggcat
     3541 tctgctgata tatttcaccg atgccattcc ggcatagcgt gccaggttgc ggagctgtat
     3601 ggcaggcagc ccagtgaaat tcaggcagga aaattccagg ctgcgcaggc gagtgtaacg
     3661 ttcaagtgct tcagtgaatg cggggccgct gatagtgaca gggcctcttt ttaggtgctc
     3721 catcactgag agtcgctgtc cttcggggat ttcaagtaac ccagccagtt gtgcggcctg
     3781 ccaacggttt ggcagcgcgg ccaattttcg ccataagcgg cgggtcgcac gctcacggat
     3841 ttcaccaatg accctcgtaa gggtggatgc ggctggcaac aacactttat tttgaagcaa
     3901 ccacgcggtg gcaaaatcga acatcagtcc ggggcgttca ttactgagcc atgcacgagt
     3961 atacagcaat cgcttcagtc tgaacgacca cgggaaatca ccgaaatcat gatagctgta
     4021 gtgctgcctg ataagcccgt ggtgctccca acgtgtattt tccctttgag catagcggga
     4081 gatgatctct ggatagcgga tgttaagttg ccttgcgaca taaaactgga caccgggcgg
     4141 tatttgcatg agatcgggca agaaggttcc cagaaagcgg gcggttgtga gttgaagggc
     4201 aatccccaac cggttatgcc tgcccctacg taggttgatg aaagtaaggt ctcgctcatc
     4261 aagatgaaaa taccgtgcca gttggatctc gtttggctct gcggcataac gcccgtagtt
     4321 ctgtgtctgt ttgtcggtca gaaaatcagc agccatcacg catcctttca ttatttattg
     4381 atccgtatta ttttggcggt atcagatatt ataatttttg agaccaatga aaacggccct
     4441 aaacataagt atcaaaaatc gtgatttatg ccttatgata ctacgcagta tcaatatcga
     4501 tttatgagac caaaatggca ttattagggt atgcaagagt atcaaccagc catcagaaac
     4561 tgacgagcca gattactgaa ctcaacacag cgggagtgag ggatgatcga gtgtttactg
     4621 acatgatgtc gggagcaact gatgagcgcg aagggctaca gcgactcctt gccagagcgg
     4681 agaaagatga cattattctc tgtactaaaa tggatcgcct tggacgtaat accgcagata
     4741 tgattcgcat cgtggatact tgctataaaa aaggcatcgc tattcgcttt cttgagaacg
     4801 ggcttagtac tgaaggcact atgggaaaaa tggttatcca gatattagca gctgttgccg
     4861 aggcggaacg tgaaaggatc ttggagcgaa ctaacgatgg gcggcaggca gctatgatta
     4921 agggcgtcaa atttggaaga aagcctcata aaaaggctga attagctaat gaattgatca
     4981 ataaggacgt acaagttaaa gaggtaatgg aaaaaacggg tatatcacgg gcgacgtatt
     5041 ttaggctgaa aagaaaaacg gttactaaca atattcatct ttccaataat aaataagaaa
     5101 gatgagcatt cttaaatgcc tgaacggctt ctatttagca atgtttaaac tcgaaaaaag
     5161 gatgtattca aattgagtca atatttaaag cttggagatg accaatctcc ggttcagttg
     5221 gacccacaca gcgaagttgg tgcatttaaa gtcgttggac actgcgcatg ggcaaaacca
     5281 gaagataaaa tcactcctga ctttttgcgt tcaagagtag aaccttggtt aactgccctt
     5341 tttcagtctg aacacttaaa tatacttatt ggcgctggcc ttagttcggc tattcaagag
     5401 tcagcgaccg gcaccaaacc tcaaggtatg ggatggattg aagatctcaa agtttgtaag
     5461 gctgaaatag acagttatgt agctaaaaca gcagacgcct caggaagggg aaagggtaat
     5521 attgaagatc agattcgttc aattaatgag ttgattaaag gattagagat tttaacggta
     5581 caaaacctac cggttccact acctccacca ggtgctcccc catatcgaaa tttaaaaagt
     5641 gagttagtag agttaaataa tgagctaaca agatgtctga aattattttc ggactcagtt
     5701 agtcatgggg aaaaattaat tcgtgatgca agcaatgatt taaaaacaga aacgtttaat
     5761 tatttagtga gttttttgat gagtttttca agtcgaacag caactaggga tcgattacat
     5821 atttttacga caaattacga cagaataatt gaaacgggcg ctgaaatagc aggtcttcgt
     5881 ttaattgaca gatttgtcgg taccatagcg ccgattttta gatcttcgcg attagaagtg
     5941 gattatcatt acaatccacc aggtattcgg ggtgaaccaa gatatttgga aggtgtggcg
     6001 cgctttacta aattacatgg ttcattggac tggtatgcta ctgaaggggc tataaggcgt
     6061 tttggattgc catttggagc tagctcggtt gaaccctttt tacaagtgga aacagccgga
     6121 actgcaaatt atcaacaatt aatgatttat ccaaattcag ttaaagaccg tgaaacatct
     6181 gaatatcctt atgttgagct atttagggat ttagcatctg caacatgtcg tcctaatagc
     6241 accttggtaa catatggtta tagttttgga gatgagcata taaatcggct tataattgat
     6301 atgttgacaa tcccctcaac gcatatagtc ataattgctt acggtgatcc gcttggaaga
     6361 attatgcgtt ttataaatga tagtggaaga aaagctcaaa tatctttatt gctgggagat
     6421 cattttggag atctaaaaaa attagttgat ttttatcttc ctaaagccgc gatagacaga
     6481 tcatcaattc gtatggctga gctattaaaa tcacgaggtt tatatcgtgc aacaaaacca
     6541 aataatagtg agga
//
LOCUS       DAOFQD010000197         6471 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.198, whole genome shotgun sequence.
ACCESSION   DAOFQD010000197 DAOFQD010000000
VERSION     DAOFQD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.198"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..450
                     /locus_tag="QBF46_004496"
     CDS             <1..450
                     /locus_tag="QBF46_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676594.1"
                     /translation="MDVTSVFRPEKMTNLINIKNRLVIHYLRTIYYEQNYIHPVGSIF
                     AAIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGILEDIMSIPDSSLRKRLGYEVL
                     SFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPI"
     gene            complement(475..1866)
                     /gene="tcyP"
                     /locus_tag="QBF46_004497"
     CDS             complement(475..1866)
                     /gene="tcyP"
                     /locus_tag="QBF46_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDQ6676595.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVLWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1999..2589)
                     /locus_tag="QBF46_004498"
     CDS             complement(1999..2589)
                     /locus_tag="QBF46_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ6676596.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2752..3420)
                     /gene="hxpB"
                     /locus_tag="QBF46_004499"
     CDS             complement(2752..3420)
                     /gene="hxpB"
                     /locus_tag="QBF46_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDQ6676597.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLADVKLSSLTELTAK
                     DLLG"
     gene            3567..4103
                     /locus_tag="QBF46_004500"
     CDS             3567..4103
                     /locus_tag="QBF46_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDQ6676598.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4144..5004)
                     /locus_tag="QBF46_004501"
     CDS             complement(4144..5004)
                     /locus_tag="QBF46_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDQ6676599.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSSNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLLADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5110..5400)
                     /gene="ghoS"
                     /locus_tag="QBF46_004502"
     CDS             complement(5110..5400)
                     /gene="ghoS"
                     /locus_tag="QBF46_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDQ6676600.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5501..6430)
                     /gene="pfkB"
                     /locus_tag="QBF46_004503"
     CDS             complement(5501..6430)
                     /gene="pfkB"
                     /locus_tag="QBF46_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDQ6676601.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1679 a         1605 c         1578 g         1609 t
ORIGIN      
        1 atggatgtca cttctgtgtt tcgcccagaa aaaatgacga atttgatcaa tataaaaaac
       61 aggttggtta ttcattattt aaggactata tactatgaac aaaattatat acatcctgtc
      121 gggagcattt ttgctgcgat tcaaaaaaat gagagtctcc taaagtttcc ttcgataata
      181 tcaatgctta atatcaatct tctttttaat ccactaaatc ttccgggaat gggaagtggt
      241 atacttgaag atattatgag tattccagat agttcactta gaaaacgtct cgggtatgag
      301 gttttaagct tctcgctaca ggcacattca ttgagccagg aatgtattga taagttggac
      361 atttttttcg ctgacgatct gtttaaatat gagtcagtat gcatcgccgc gatggaacat
      421 ttaaaaagta aggcaacggc ccctatatga aacggaccgt tgccagcata agaattaatg
      481 gtgtgccagt tcggcgtcgt cttcgctatc cagaatggct ttatcggttt gcttcagcca
      541 ctggctggtc agcgtgccag ctgtcatcga gccactaacg tttagcgccg tacggcccat
      601 gtcgataagc ggttcaacgg agattaacag cgccaccagg gttactggca ggcccatcgc
      661 aggcagcaca atcagtgcgg cgaaagttgc accaccaccg acaccggcaa cgcctgcgga
      721 gctaacggta acaataccga ccagcgtcgc aatccacatc gggtccagcg ggttaatgcc
      781 aaccgtaggc gcaaccatca ccgccagcat tgccggatac aaaccggcgc agccgttctg
      841 accaatggtc gcaccgaaag aggcggcgaa actggcgatg gattcaggaa cgcccagacg
      901 acgcgtttgt gcttccacat tcagtgggat agacgcagca ctggaacggc tggtaaaggc
      961 aaacgtcagc acaggccata ccttacggaa gtacttcagc ggactcacgc cattaatgcc
     1021 cagcagaatg ccatgcactg caaacataat cagcagaccg aggtaggacg cgacaacgaa
     1081 acttcccagt ttgatgatgt cttgcaggtt agaacctgca accactttgg tcattagtgc
     1141 cagaacgccg taaggggtca actgcatgac caggcgaacc agtttcatca cccagctttg
     1201 cagggtatcg atagcggtta agacgcgttc acctttcggc gcatcatcct tcagcagttt
     1261 cagcgcagct acaccgagga atgcggcaaa aattaccacg ctgataattg acgtcggatt
     1321 ggctccggtc agatcggcaa acgggttttt cgggataaag gacaagacca gctgcggaac
     1381 gctcaggtca gagactttac caacatagtt actttcaatg gcgttcagac gtgcagtttc
     1441 tgcaccaccc tgaaccagac cttcagccgt caaaccaaac aggttggtga ccagcacacc
     1501 gaccagcgcc gcaatcagcg tggtaaacaa aagcgtaccg atggtcagaa aactgatttt
     1561 gcctaactga gatgcgttat gcagacgggc aaccgcgctc agaatagagg cgaagactaa
     1621 cggcataacg atcatttgca gcagttgaac atagccgtta ccaacgatgt taaaccacag
     1681 tacagaatct ttaagtacct ggctgtcaga accataaatg gtatgcaggg caaggccaaa
     1741 aaccacaccc atcaccagac ccaccagcac ttttttcgcc agactccact gtttatggcg
     1801 ggtctgagcc agcgcaaaca gcagtacaac gaacaccacg atgttcgcaa ttaatggaaa
     1861 gttcatcccc gttctcctga tcttattatt tctatcactg cgtagcagtc atagtgttgg
     1921 cgcaagatta acagaactgt gagctggctc ttatattcaa atggaattgc ttattacaga
     1981 aaggtgattt ttgccttttt agtattcaac ctgatgctta ataagccgat gaaactggat
     2041 ctgcaaggta ttaatcattt gcgatgacca acgaacagcg ctgttttcgg gtaatgaatg
     2101 gggcatccat accgaccaga caaataatgc catgcagata aaacgttcca gttggttgcc
     2161 cttttgggga accaggatag gtaagcggaa acgccagcgg catggccaga gcaggggaac
     2221 accggcgggt gtcagcatat cggcaagtat gtggcttaaa taacccagca ccattccttg
     2281 tagcgcatca gccggaatga accagctttc aggaacctta aggtaaaagg ttgccagcag
     2341 cgcaaatacc gccagcagac tgtgggtaaa accacgatgc ccaaaagcgc gggcgatcgg
     2401 ttttgatatc cactttaatc gttgcccaag aaacgacttt gggtgatcga tgtccggtaa
     2461 caaacacgtc aggattgcgg aagggacaat atgccaccag tcaccctgtg ccagcacggg
     2521 cgtcagctcg gcatttttgg caaataccgc acaagcaata gaaaagagaa ggtgaccttc
     2581 cgccgtcatg ataaaactca ctaaactgtc gatacgtaca gtataggatt tttatacagt
     2641 gcacggaagt ggtgacggtg taactctttc tcttaaattg acgattaatc cgtcagttga
     2701 atgcaaattc agataaagaa ccaggggcag cgagatcact gcccctggtc attaaccgag
     2761 aaggtctttt gcggtgagtt ctgtcagcga tgagagtttg acgtctgcta atacaaaacg
     2821 tggatcattt tgcgcttctg gcgcaggaac gacgatggaa cgcatgcggg ctgctttaga
     2881 ggcgatcatg ccatttaccg agtcttccag cgctacgcag gtcagtgggt caacgcccag
     2941 ttttgctgcg cagtcgagat atacttgcgg atgcggcttg ctgtaaggca gtttttcagc
     3001 cgaggcgagg gcatcgaaac tgtcgcgtaa gtcaaacatg gtcaacactt tttccagcat
     3061 atgtagtggt gacgcggagg ccagccccac caataaacct tgttctttgc ataacgcaac
     3121 ggcttcgcgc acgcctggta ataatggacg tgtctcttca accagtgaaa tggcacgggc
     3181 aataacccgt tctactactt cctgacggct tggcccattc cacggttgcc gggcgtacca
     3241 aagatcgacc accatatcga tgcgtaaacc taaggtgtcc ggcagctcgt tacgacggga
     3301 gatatccacc cccaggcttg ccatcacatc cagttcggct cgatcccata aaggttctga
     3361 gtcgataagt aatccatcca tatcaaaaat tgcagcaaga atctgacgcg gggttgacat
     3421 tacaacctct cctttagagc attatgtgta aggtattaag ccgtgcttat gattaagcgc
     3481 ctatcatatc gcctttaaag aatgggcgaa aacagatatc tacaggtaaa ctaagtgaaa
     3541 cgacgcgtct taatcaagga gaactcatga cgtatcaaca agctggacgc attgctgttt
     3601 tgaaaaggat tttgggttgg gtgattttta tacctgcatt gatctctaca ttgatttctt
     3661 tgctgaaatt tatgaatacc cggcaggaaa accaggaagg cattaatgcg gtcatgctcg
     3721 actttactca tgtcatgatc gatatgatgc aggcgaatac gccttttttg aatctgttct
     3781 ggtataactc cccgacccct aattttaatg gtggcgtgaa cgttatgttc tgggtgattt
     3841 tcatcctgat ttttgtcgga ctggcgttgc aggattccgg tgcccggatg agtcgccagg
     3901 cgcgcttctt gcgggaaggt gttgaagatc aactcattct ggaaaaagcc aaaggggaag
     3961 aggggctaac gcgtgaacaa attgagtccc gtattgttgt tccgcaccat actattttcc
     4021 tgcagttttt ctcactgtat atcctgccgg ttatttgtat tgctgcaggt tatgtattct
     4081 tttctctgct tgggtttatt taatcaaaag gccgcacgga gcggccatta tcctcaaccc
     4141 atatcatgct gctaataatc tatccaatga ctgctgagca ataaccaaat gctgaccgcc
     4201 aaataatctt gcacgattta acagcgtgta gagttggtaa accggttgac gttcaaggaa
     4261 atcggcaagt agcggggata ctgactgata gccgtcatag atttgtggcg gctgttcagt
     4321 atgcagcggt aacatcgcca ggtcgcactc tcggtcaccc cagtagcagg cagggtcgaa
     4381 aatgtacggg ccatccggac ccagcgcaca gttgctggac cataaatcgc cgtgcaacag
     4441 agaaggctgc ggttgatgcg aggccagacg ctgctgaata tgctcgacga tggcgtcgat
     4501 attgccgaaa gcgatccctt tctctgctgc cagttccaac tgccagccaa tccgttgttc
     4561 agcaaaaaac gttgaccagc gacgttgcca ggtgttgggc tgtggagttg tggagagcga
     4621 gttatcaaaa tcgaggccaa attgtggttg gtcactccat tgatgtaaac gcgcaatttg
     4681 ctgaccaaga ataaatgcgc tatgcgcatc cagcggacga ggtgggagat aatccatcac
     4741 cagaaaactg tagtcacggt cagcgcctac tgcccaaacc ttaggcacgg tgacggtttt
     4801 actacgcgac agtaactcca gttggtcggc ttcggcggta aaaccgggaa gcagttccct
     4861 ttcatcacat ttgacgaaaa agtcatgtcc tgcatagcgc aaatgccatg cggcatggac
     4921 ttctccgcca ggcagttcat tacgcagttc gatttcgcct tcacctaact gctcgctcaa
     4981 aagacgactg attgcctgcc acatgatgtc tctcccatgt tgtctgccag atcataaggt
     5041 tagcgcaaga atgcggtgta aaaacacgag ctaacgcaca ccacagactg attattgccc
     5101 aaaaaccatt tattttctgt tgctattcca ttcctcccag gtcgtgatgg tgatttcagg
     5161 atctttgccg gttgcacttt gggtaagccc cgaaaccagt tctctaattt cttcttcact
     5221 ttgggtactg ataagtccaa aagtgttagt ccccagctca tggatatttc cctcatcatc
     5281 cgtcatggtg agcaaaaaac cgtcgcgagt caggtaatta ttcaactcgt taatttcagt
     5341 caatgtatcc tcatgcaaca tgacggttat gacataacgg acaagatcgc cactggccat
     5401 agttcacccc tttgttatca catgactttt gagcatagtc ggagaaacgc gttgccgaca
     5461 ggttggtggt gattccccca atgctggggg aatgtttttg ttagcgggaa aggtaagcgt
     5521 aaattttttg cgtatcgtca tgggagcaca gacgtgttcc ctgattgagt gtggctgcac
     5581 tccccgcagc tacgccaaaa cggaccatct cttcaagaga ggcattttct gccagtttca
     5641 gtgtcatcgc gccgaccatg ctgtcaccag cgccaacggt actctggctt ttcaccggtg
     5701 gtggcaccac ctgaatgcag ttttcactat caacacccag cgctccttgt ggaccaaggg
     5761 aaacgacaac ccgtttggcc ttgccgctat taacgatttc ctgcgcggct ttgcggacat
     5821 cgtctggctg ggtgagttcg cgattcacca gcgcactgag ttctttttgg ttaggcttaa
     5881 ccaactcgat gttaccaatc gccagtgctg cacttaacgc ttcgccagaa ctgtcgatga
     5941 tgcagcggat cccttgtttt tgcgcagcgg aaatcagttg ggttaatttt tccagcttca
     6001 cacctggcgg caggcttccg cttatgacca ggatggcccc ggattcaatt tccagaactt
     6061 gctcttcaag ctggcgaaac tcatcttcat ttaatgccgc gcctggcata acaaaacgat
     6121 actgctcacc gcttgcttcc acatgtacgt gtaaattctg ccgggtccag tctttggctt
     6181 ctacagtagc gacggggaca ttttcatccg ccaacagtga aaccaggtgt tcgccggtcg
     6241 cgccacccgc cgggaagatc gctgtggcac tgcctccaag atgggcaatg gcgcgggcga
     6301 cgttgatgcc gccacccccg ggttcgaaca ccggtgcggt acagcgcagt tttccttcgg
     6361 gataaatttg cggggtaatt gttgcgctat cgagagaggg cgcaagtgtc aacgtataga
     6421 tacgtaccat catttcctcc tataggctga tttcagtctg gcaccgaatc a
//
LOCUS       DAOFQD010000198         6170 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.199, whole genome shotgun sequence.
ACCESSION   DAOFQD010000198 DAOFQD010000000
VERSION     DAOFQD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.199"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            200..1294
                     /gene="ddlA"
                     /locus_tag="QBF46_004504"
     CDS             200..1294
                     /gene="ddlA"
                     /locus_tag="QBF46_004504"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDQ6676602.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHERQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1318..1530)
                     /locus_tag="QBF46_004505"
     CDS             complement(1318..1530)
                     /locus_tag="QBF46_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDQ6676603.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1790..2098
                     /locus_tag="QBF46_004506"
     CDS             1790..2098
                     /locus_tag="QBF46_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDQ6676604.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2157..3251)
                     /gene="yaiW"
                     /locus_tag="QBF46_004507"
     CDS             complement(2157..3251)
                     /gene="yaiW"
                     /locus_tag="QBF46_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDQ6676605.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3264..4484)
                     /gene="sbmA"
                     /locus_tag="QBF46_004508"
     CDS             complement(3264..4484)
                     /gene="sbmA"
                     /locus_tag="QBF46_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDQ6676606.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4836..5993
                     /gene="ampH"
                     /locus_tag="QBF46_004509"
     CDS             4836..5993
                     /gene="ampH"
                     /locus_tag="QBF46_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDQ6676607.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGTRVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5994..>6170)
                     /locus_tag="QBF46_004510"
     CDS             complement(5994..>6170)
                     /locus_tag="QBF46_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6676608.1"
                     /translation="IDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMN
                     KGKLVAINRLPSEY"
BASE COUNT         1537 a         1604 c         1523 g         1506 t
ORIGIN      
        1 catttaagcc aggaataaat tagcttcccg ttcatcattt ttgtgatctt gcgcacatct
       61 ttgagataac gcactttctt caccagtacg ttgcacgctt tgtcagcaga agattgcaac
      121 aatctttcag aaggccgcga atcccccgtt ttgtcgtggt aatgtatgca ctttgtgtta
      181 gacgggatag gtttttaaga tggaaaaact gcgggtagga atcgtttttg gtggtaaatc
      241 agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt gtcgatgcca ttgataaaag
      301 tcgcttcgac gttgtgctgc tgggcattga taaacaaggg caatggcacg tcagcgatgc
      361 cagcaattat ctgctaaatg cagacgatcc tgcccatatt gcgttgcgcc cttcggcgac
      421 cagccttgcg caggtgccag gtaaacatga gcgtcagctt atcgacgcgc aaaacggtca
      481 gccgttgccg acggtggatg tcattttccc gattgtccac ggtacgctgg gcgaagatgg
      541 ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt gtaggttctg atgttctggc
      601 ttcagcagcc tgtatggata aagatgttac caaacgtctg ctgcgcgatg ccgggctgaa
      661 cattgcgcca tttattaccc tgacgcgcgc caatcgtcac aacattagtt ttgccgaagt
      721 ggagtctaaa ctggggttac cactgtttgt aaaaccggct aatcagggct cttctgttgg
      781 tgtcagcaaa gtaaccagtg aagaacagta cgcaattgcc gtcgatctgg cgttcgaatt
      841 cgatcataaa gtgatcgttg agcaagggat caaaggtcgt gagatcgaat gcgcagttct
      901 gggcaacgac aacccgcaag ccagcacctg tggcgagatc gtactcacca gcgacttcta
      961 tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa gtggtagttc cggcagccat
     1021 tgcgccagaa atcaacgata agatccgggc gattgccgtt caggcttatc aaacgttggg
     1081 atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca gagaacgaag tggtgatcaa
     1141 cgagatcaac acactgcctg gcttcaccaa catcagtatg tatccgaagc tgtggcaagc
     1201 cagcggtctc ggttacaccg atctgatcac acgtttgatt gaactggcgc tggagcgtca
     1261 cgctgcggat aacgcattga aaaccacaat gtaatatttt aacgcccgat aatattctta
     1321 ttgggcgttt tcagtttctt catcgcgacg acgaataatc agccccgcca gccagaaact
     1381 aatcacccac gtcaccagac cgacggcata ggtctgccaa ccttttgctt caaatcccag
     1441 tagccccacc acgccattaa gaatgaatat aaggccaatg gcgaacgcat agtagtgcca
     1501 gtcacggcgg atttttacag gcagattcat ggcagctccg ttaacgaaaa acaacatcct
     1561 tgcatattct gtaacaaccg tctgtggtgt agacaacatt ttcgtaattg ttaataaatt
     1621 taacttaagg ttgccatttt gtgactcaca acgaagattc acgcttcggg aatattccca
     1681 acatcaattt accatgattc tgatattgac aatctaaatg acctgtcaga cttgccataa
     1741 gccgctggta tcacaccaga agagaagcag atgacttccg gaggttttta tggctgattt
     1801 caccctgtca aaatcgctgt ttagcggaaa atatcgcaat gcctcttcaa cgcctggcaa
     1861 cattgcctat gcgttgtttg tgctgttttg cttttgggct ggggcgcaat tgctgaacct
     1921 gttagtgcat gcgcccggcg tctatgagcg tttaatgcag gtccaggaaa caggtcgccc
     1981 acgggtggaa attggtttag gtgtcggcac cattttcggg ctgatcccgt ttttagtagg
     2041 ctgcctcatt tttgcagtgg tggcgctatg gctgcactgg cgacatcgcc gccagtaaga
     2101 tttacgttgc cagatacggc ctaaaacgcc ttatctggca tgacattcct gtttgatcac
     2161 tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg caaaccaggc cgtagtcagg
     2221 ttgcgggtga ttttcgggct ttccagttga atgccaggca acatttcgcg gggtagtgat
     2281 ttacccgttt tcgcttcggc aagttgataa actttcttgt acagcgccgt ctcctcaaaa
     2341 gagaagctgt cacctttctc taactgacgg cgaatttcgc tgtcgctcat ccccagtttt
     2401 cccgccagtt tgcgcgttgc cagttcggtt ttcccgggtt ctttactgtc gtaacgaatt
     2461 aaatcaccgt ccagtgccag cttcacgccg ctggccttac tgactgcgtt ctgaaacgcg
     2521 gcattacggc tggcgtacca gccagcgtta aaatcagcaa agcggtatat cggtgcgcta
     2581 tagctggcgg gataattcag taaatggtaa gtaccaaacc acaacccgcc gcggcgactg
     2641 aagacttcct gacgaactgt accgtccatt ttccacggat accctttggt atgctgttcg
     2701 gcaaaagcaa tgctgacctg catcggccca ccggtgcgca ccggattaag cgaaccaaac
     2761 agcgtctgcc ccattggtac catgctgatc agatcgtcaa aaatcgcgct caattgcttt
     2821 tctgtacgca ccgaatctaa ccgttcgcta taacttttgc cgtttggcga tttgattttt
     2881 agcgcggtat gaaccagaaa agcaggaatg tgcatccgtt cagcacgacg gtcaatctct
     2941 tgccaggcga tcttgcttaa gcccggaacg gccggatctg cctgataatt tgactcctgc
     3001 tgcgccaccg ccagcaccga gcagacgttt tccagcgtcg gtgccagccc ctgactttca
     3061 aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt tcacgctcgc aggcatcttc
     3121 tgccgcacga cgctcgccac atctatcgct ttttcgccct ttttcaacgg ttgcggagcc
     3181 tgactactac accccgctaa aaccagaacc gccaacaatg acagcgaaga tagatgattg
     3241 acgcgcgaca ttacttctcc tttttagctc aaggtatggg ttacttcctg aattttgtca
     3301 ccatccagct catgttcaaa gctgcgcaga cgtttgtaga tagacatcaa ctcaaccagt
     3361 gtggtccatg agttaatcag gtactggaaa gcaccgcgaa cctgaccaaa aacgttggta
     3421 atctgcgtca tcaggccgag cgtaatcgta ccggcaacaa ttgacggaaa cagcaagaac
     3481 aaaccgaaaa cgttatcgac ctgcaaatag agaatgcggg cgatgttgaa atacatatag
     3541 tgaaaataga ggcgaaaata gtttttccgt acggcgctaa acagctcgcg taccgtaggc
     3601 ggcgtcgcgc gcgtggcatc gtcttcacca taaaccagct ctttacggta ggcggcctct
     3661 actcgctggt ttttaaactc cagccccggc agtttgatcc ctaccactgc cagcaatccg
     3721 gtccccatca gcgaccagac gatagcggca atcaccagac catacggaat gtgcccgata
     3781 atcggcagct ccggcacatg cgcggagagc gttaccagca ccggcaggaa ggcgatcaac
     3841 gtcatgatgg cgttgataaa actgacgccc atattctcca gcgttgaagc aaaacgcatg
     3901 gtgtcttcct gcacacgctg tgcggcacct tcgatatgac gcagttgttg ccagttcgcc
     3961 atgtaatatt cgttcatcgc cgtacgccag cggaacacgt agtgactgac aaagaagttg
     4021 ttcagtacac tgatcaccac cgcgatcagc gcaatcccca gaaagacgcc cacttcgcgg
     4081 taaaattgtt cgatagtgac tttatgcggc gaacttagcg cggtttgaat cagatcatag
     4141 aacggtgcat accaggcgtt gacggcgacc ccgacttcca ccaaaaacca ggtgacgaag
     4201 atgatcagtg cagtaccgag tattgaccag tattgccaac ggtgtgggct gtagataaac
     4261 cagaacaatg caaaaagtcc tacgcaaaca atgtagtaag cgtaaaaaat caggaaatcc
     4321 aacgaccaga aacgcgcggc gctaatcggg atctgcccgg aagcgccggt gatacgcgcc
     4381 acccagtcac ccccaccggc ttgccagaag ataacggcaa tcaatgccca aacaaaggcc
     4441 gagagaaaaa acgttcccgg ctttgggaaa aaagacttaa acatatgcac tcctgctaac
     4501 ttcttatcgt tttaacaact gctgtgtatt aaccgcatga ccgctacaaa gcgtcagcca
     4561 aatgaaaccg cccattgtga cccatagcaa ggcgcttagt tcgccagcct gaccataaga
     4621 attgtttcga cggatttcac aaaccaaaga ttgtttacat aagcggatta acacgcgcag
     4681 gatcacaaat gcaggcattg gtgagcagga aaaagatcgt gtttacgcgc cgttacgttt
     4741 ttaacactat tattgcacta attcgccctt tgcaatctat caatgagtag tataaatacg
     4801 ctcagttacc ttcattcaat ctatggacac caccgttgaa acgtagtctg cttttttctg
     4861 ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc gcagccgatc actgaaccgg
     4921 agtttgcctc tgatattgtc gatcgttatg ccgatcatat tttttacggc agcggtgcca
     4981 cggggatggc gctggttgtt atcgacggta accagcgcgt ctttcgtagt tatggcgaaa
     5041 cgcgacctgg taataacgtt cgcccgcagc tggattcggt cgtgcgtatt gcttctctca
     5101 ccaagctaat gaccagtgag atgctggtga aattgctcga ccaggggacc gtgaagttaa
     5161 acgatccgct aagtaaatat gctccgccag gcacacgcgt accaacctac aatgggacgc
     5221 ccattacgct ggtcaatctg gcaacccata ccagcgccct gccccgtgaa cagcccggtg
     5281 gtgcggcaca tcgtccggtg tttgtctggc caacgcgcga gcaacgctgg aaataccttt
     5341 ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc gtactctaac cttgcgttcg
     5401 atctgctggc agatgcactg gcgaatgcgt ccggcaagcc ttatacccag ttgtttgaag
     5461 agcagattac ccgtccattg gggatgaaag acaccaccta caccccctca ccggatcagt
     5521 gccgccgttt aatggttgca gagcgcggtg ccagtccatg caataacacg ctggcggcaa
     5581 ttggcagcgg cggcgtctat tccacgcctg gcgatatgat gcgctggatg cagcagtatt
     5641 tgtcatctga tttttatcag cgcagtaacc aggcagatcg catgcagacg ttgatttatc
     5701 agcgcgcgca gtttacgaaa gtgattggca tggatgtccc cggcaaagcg gatgcgcttg
     5761 gtttaggctg ggtatatatg gccccgaaag agggtcgtcc ggggattatt cagaagacag
     5821 gtggtggtgg cggtttcatt acctatatgg cgatgatccc gcagaagaat attggcgcgt
     5881 ttgtggtggt cactcgttcg ccgttgacgc gctttaaaaa tatgagcgat ggcatcaatg
     5941 acctggtgac cgagctaagc gggaataaac cgctggttat ccccgcatcc tgattaatac
     6001 tctgaaggca aacggttgat agcgaccagt ttgcctttat tcatttcgat atagccgcct
     6061 ttacgcaaag cagcgagaac ttctgcgacg acagaacgcg atatgcgtgt acgttgatgg
     6121 atataattca tcacaccaat acgtgatcgc aattcttcat tccagtcaat
//
LOCUS       DAOFQD010000199         6009 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.200, whole genome shotgun sequence.
ACCESSION   DAOFQD010000199 DAOFQD010000000
VERSION     DAOFQD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.200"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1472
                     /locus_tag="QBF46_004511"
     CDS             <1..1472
                     /locus_tag="QBF46_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDQ6676609.1"
                     /translation="TDPQEAIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTI
                     SPLASLDEPDSLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDL
                     PVSISAVLMAEACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQA
                     TLPLAQIWGGGEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHG
                     IVIPGTLRDSIFVLEGLLEQETGLNPTEIMTDTAGTSELVFGLFWLLGYQFSPRLADA
                     GASVFWRMGHDANYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSL
                     LKSERPSGLTQAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHG
                     QKGEIRKRYTDGQEDQLGALGLVTNAVVLWNTMYMQAALDHLRAQGETLNDEDIARLS
                     PLCHGHINMLGHYSFTLAELVTKGHLRPLKEASEVENVA"
     gene            1761..2521
                     /locus_tag="QBF46_004512"
     CDS             join(1761..2085,2085..2521)
                     /locus_tag="QBF46_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611975.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn12 family transposase"
                     /protein_id="HDQ6676610.1"
                     /translation="MSNLFWLTDEQMARLQPYFPKSHGRQRVDDRRVLSGIIFVNRNG
                     LRWRDAPKEYGPAKTLYNRWKRWGDKGVFIQMMEGLAVPEAAEHKTIMIDATYLKAHR
                     TASSLRGKKGGAGRLIGRTKGGMNTKLHAVTDASGRPISFFITAGQVSDYTGAAALLD
                     ELPKAKWLLADRGYDADWYRDALQAKGITPCIPGRKSRKKTIKYDKRRYKRRNRIEIM
                     FGRLKDWRRVATRYDRCPMAFLSAIALAATVIFWL"
     gene            3413..3598
                     /locus_tag="QBF46_004513"
     CDS             3413..3598
                     /locus_tag="QBF46_004513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676611.1"
                     /translation="MRKDQIPDFVDEIFATGCPITAVGQDCYVLQRAICWQKTSTVLV
                     RWSIKSASDKESVTICC"
     gene            complement(3762..3953)
                     /locus_tag="QBF46_004514"
     CDS             complement(3762..3953)
                     /locus_tag="QBF46_004514"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676612.1"
                     /translation="MVIGFIGVLLVVKPGYLPFQLGHLAAIGTGLATDAAIIILRRVG
                     GKVKTTSMLGISARRLQRG"
     gene            complement(4535..5077)
                     /locus_tag="QBF46_004515"
     CDS             complement(4535..5077)
                     /locus_tag="QBF46_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6676613.1"
                     /translation="MIIWINGPFGAGKTTLAERLRDRRPKSLIFDPEEIGFVVKETVP
                     IPASGDYQDLPLWRGLTIAAVSEIRRNYSQDIIIPMTLVHPDYLTEILDGLRQIDDQL
                     LHIFLMLNEDLLRHRISNQTMHPDPNRNAEIREWRLANVARCLAARERLPSTTRVLDS
                     GAHTSDELAAMVLDRLDQRT"
     gene            complement(5090..5950)
                     /gene="aac(3)-IIe"
                     /locus_tag="QBF46_004516"
     CDS             complement(5090..5950)
                     /gene="aac(3)-IIe"
                     /locus_tag="QBF46_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IIe"
                     /protein_id="HDQ6676614.1"
                     /translation="MHTRKAITEAIRKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDKARRTWPPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRNGEVAWKTASEYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGATPIVPAHE
                     AAQRSCEPSG"
BASE COUNT         1310 a         1667 c         1682 g         1350 t
ORIGIN      
        1 cgacagaccc gcaggaagca ataaaatctc tgggtcatca gcttgatagt cgttacagac
       61 aggttgctgc acgtcttggc gaaaatgagg ctgtcgaact cgatgtttct ggcccgaagc
      121 cccggttgac aatttctccc ctcgccagtc ttgatgagcc ggacagtctg aaacgactga
      181 gcaaaatgat cagtgatctg ctccctccgg tggatttaac ggagttgctg ctcgaaatta
      241 acgcccatac cggatttgct gatgagtttt tccatgctag tgaagccagt gccagagttg
      301 atgatctgcc cgtcagcatc agcgccgtgc tgatggctga agcctgcaat atcggtctgg
      361 aaccactgat cagatcaaat gttcctgcac tgacccgaca ccggctgaac tggacaaaag
      421 cgaactatct gcgggctgaa actatcacca gcgctaatgc cagactggtt gattttcagg
      481 caacgctgcc actggcacag atatggggtg gcggagaagt ggcatctgca gatggaatgc
      541 gctttgttac gccagtcaga acaatcaatg ccggaccgaa ccgcaaatac tttggtaata
      601 acagagggat cacctggtac aactttgtgt ccgatcagta ttccggcttt catggcatcg
      661 ttataccggg gacgctgagg gactctatct ttgtgctgga aggccttctg gaacaggaga
      721 ccgggctgaa tccaaccgaa attatgaccg atacagcagg taccagcgaa cttgtctttg
      781 gccttttctg gctgctggga taccagtttt ctccacgcct ggctgatgcc ggtgcttcgg
      841 ttttctggcg aatgggccat gatgccaact atggcgtgct gaatgatatt gccagagggc
      901 aatcagatcc ccgaaaaata gtccttcagt gggacgaaat gatccggacc gctggctccc
      961 tgaaactggg caaagtacag gcttcagtgc tggtccgttc attgctgaaa agtgaacgtc
     1021 cttccggact gactcaggca atcattgaag tggggcgcat caacaaaacg ctgtatctgc
     1081 ttaattatat tgatgatgaa gattaccgcc ggcgcattct gacccagctt aatcggggag
     1141 aaagccgcca tgccgttgcc agagccatct gtcacggtca aaaaggtgag ataagaaaac
     1201 gatataccga cggtcaggaa gatcaactgg gcgcactggg gctggtcact aacgccgtcg
     1261 tgttatggaa cactatgtat atgcaggcag ccctggatca tctccgggcg caaggtgaaa
     1321 cactgaatga tgaagatatc gcacgcctct ccccgctttg ccacggacat atcaatatgc
     1381 tcggccatta ttccttcacg ctggcagaac tggtgaccaa aggacatctg agaccattaa
     1441 aagaggcgtc agaggtagaa aacgttgctt aacgtgagtt ttcgttccac tgagcgtcag
     1501 acccctatac ctatcgagat atcactctac ctctcgctgc ttgaccgagg tggaggaccg
     1561 tcgccgttgg tggctttttg gccaagcgtt tatccgcgtc gtctcgccgc ccgctccgcc
     1621 tccgcgcgaa tagcaagcag cgaaggacca agaagctcac gcgcatgagc gaccgtttcg
     1681 gcggatgccg tgtttagggt caggacccat tgatttgaac tgacggctgt gattcagatg
     1741 ggcgtatagg agcccgactg atgagtaatt tgttttggct gacggacgag caaatggctc
     1801 gtcttcagcc ctatttcccc aagagccatg gccgccagcg cgttgatgat cgacgcgttc
     1861 tgagcggcat catcttcgtc aaccgcaacg ggctgcgttg gcgtgatgcg ccgaaggaat
     1921 atggcccggc gaagacgctt tataaccgct ggaaacgctg gggcgacaag ggcgtattta
     1981 tccagatgat ggaaggtctg gccgtgcctg aagccgcaga gcacaagacc atcatgatcg
     2041 atgcgaccta tctcaaggcc caccgcacgg cttccagctt gcgggtaaaa aagggggcgc
     2101 gggccgcctg attggacgca cgaaaggcgg catgaacacc aagcttcatg ccgtaacgga
     2161 tgcgagtggt cgcccgatca gcttcttcat aacggcaggc caggtcagcg attacaccgg
     2221 tgctgccgcc ttgcttgatg aacttcccaa ggccaaatgg ttgctggccg accgtggcta
     2281 tgatgccgac tggtatcgtg acgctttaca ggcgaagggg atcactccct gcattccagg
     2341 tcgaaaatcc cgcaagaaga ccatcaaata cgacaaacgc cgctacaaac ggcgcaaccg
     2401 gatcgagatc atgttcgggc gtctcaaaga ctggcggcgt gtcgctacgc gttacgaccg
     2461 gtgcccaatg gcctttctct ccgccatcgc tctcgctgcg accgttatct tctggctatg
     2521 agcaatgagt cctgacccta gcgcatatca agccagagga gcctgaggta cttggtcttt
     2581 tagccctcat ggaactccag tgctctcggc tgcttgcccg acaagatcgt caaggcttgc
     2641 cagtgccgct cgatcgtcag gatcgtggct tgtgggatcg attgctcata cgtcgaggtc
     2701 tggagatagt tgagcgagca tgccgcatgt cgtctcccac tggatggtat cttctgcaag
     2761 cgttgatttc tgcttgtcac gcgcgggctg cgagctttcg agacaccaat tggcgagaga
     2821 tactggcgag atatgacgcg ctcttcctgt tgagccctac atacgtagtt gccctcaatc
     2881 gagcagtcgc tgtgtcgtgg gccatgaacc ccgctgcagg gatggcggtc cttaaggcga
     2941 ttgaggacga gtgggacgtg gagacctacc cgctttttca cgcgacgcgg gcggatttcc
     3001 tttcgcgtct tggtttccaa gaccaagctg cggcagccta tcaggctgcg gccgtactca
     3061 gcaccaacct acctcaaaag ttgttcttgg tcacgcgcgc tgaggaatgc ctggcagggt
     3121 ctcggacaga tgttggataa catcagtcga ttgacggtga ttgcgcgcac gtggacgcgc
     3181 atcatgacag gtatgacgag cctttgaacg tccgctggct gtgccggcgc catcatatcc
     3241 ggctgcactt ctacggtgag gacatgtttc ctatgtgcag gccgcatcac gtatgaacgg
     3301 cggctctccg gttatgcctc aacgccgctg aaagcgtcgc gcggtcagtc agccatgacg
     3361 atcatacctt attcagatca tgcgatcggc gccctgccat caggaggatg aaatgcgtaa
     3421 agatcagatc ccggatttcg ttgatgagat cttcgccacc ggctgcccaa tcaccgcagt
     3481 aggacaagat tgctatgttt tgcagagagc gatctgctgg cagaagactt cgaccgtgtt
     3541 ggtgaggtgg tcgataaaat ctgccagcga taaggaaagc gtgaccatct gttgctgaca
     3601 tcgtcgatca tttgaggagc ctcggccgtt tctacgacct gcggcttgag acactccaat
     3661 agcgactatg ggtcggtccg cgctttagaa taagggatct agaccctgtc gccaattttg
     3721 gcctgacggg ggtttccagc tacgccttca tatgtcgtgc ctcagccgcg ctgcaagcgc
     3781 cgggcactga tgcccagcat gctggtggtc ttcacctttc cgccgacgcg cctgaggatg
     3841 atgattgccg cgtccgtggc cagcccggtg ccgatggcag ccaaatgacc caattggaac
     3901 ggcagatagc ccggcttgac cacgagaagc actccgatga agccgatgac cacggcggtc
     3961 caccgccgaa ccgcgatgct gaagccacaa atcgagcttt gcgaggagtt tctttatccg
     4021 tctcaatgag ataagggcca actttagatc aagtgcgtcg tagtacggcg tgcaccttgg
     4081 gcttgatcca gctatagtgt aattgtatgc tggaaatatg tgcaaccctg gtgtctaggc
     4141 cgtgcgaagc caatcaggct cgaaagtcgt tgcgggttgt tatccgttaa aggcggcgac
     4201 tgtagccgcc gctatttggc cccttgatac catttagcat ttggagcttt aggttctata
     4261 atttaacgtt acgctacagc cgacaccccg cttgcctgag cgtttggatt tcaatcactt
     4321 agtagaccaa cttgcgggtc gacggttcgt gtcccttcaa ggcggaggct tcggcagctg
     4381 gcgtctctat gccgcggtgg tgcttttccg aaacggaagt cgcccattcg cgtcacgtaa
     4441 accgaccgtc aggcggtacg gcttaggaga ataatcgcaa agcagactga gaccaccacc
     4501 ctcgcgctac cctttgcgca ggcgtcgaag gcgatcaggt gcgctgatcg agtctgtcga
     4561 gcaccatcgc tgcaagttca tcgctggtgt gtgcaccact atcgagaaca cgggttgtgg
     4621 acggaagccg ttcccttgcg gccaagcatc gggcgacatt cgctaatcgc cactctcgaa
     4681 tctccgcatt ccgattcggg tcaggatgca tggtctggtt cgagatccgg tgacgcaata
     4741 ggtcctcgtt aagcatcaga aagatgtgca gcagctgatc gtcgatctgc cttaacccgt
     4801 cgagtatctc agtcagatag tccgggtgca cgagcgtcat tgggatgatg atgtcctgcg
     4861 agtaattcct gcgaatctcg ctgaccgcag cgatcgtaag tcccctccac aaggggagat
     4921 cctgatagtc tccgctcgct ggtatgggga ccgtttcttt caccacgaac ccgatttcct
     4981 cagggtcaaa gatcagcgat ttcggacgcc gatcgcgcag ccgctcagcg agagtcgtct
     5041 ttccggcgcc gaaaggtccg ttgatccaga ttatcattgt cgacggcctc taaccggaag
     5101 gctcgcaaga gcgctgggcg gcttcgtgtg ctggcacgat cggagtggct ccgaagtgct
     5161 tctcaagata ggtgacgccg aacgtcacga tgtcctgcgc gtcgaacagg tagcactgag
     5221 caaagcccac gacaccttct cgatggcgac cgagcttcac gtaagcattt gctatagttt
     5281 cgaccgcatc cggctttcct tcgatagcaa agcaatcgag aatgccgttt gaatcgtatt
     5341 ctgatgccgt tttccaggcg acttcaccgt ttcttccaag catcggcatc tcatacgtca
     5401 cccatcgttt gttggggata tccgcaaccg cctcggcgta gtgcaatgcg gtaacggagt
     5461 ttagcggcgc acccaacagc agggccttcc cgccaaggcg gacgaaccgc tcgacgggcg
     5521 acccttcccc caaggcgtga ccgagttcgt gaggctccgt cagcgtttca gctagcggac
     5581 caaccgcgac catcgatgca tcggggtgcg cgctgcgccg cgcgccgggg gcttgaacca
     5641 gaaattgatt cagcaggccg aacccacggt aagtcccggc cgttgcggga tcgaacggcg
     5701 gccaggtacg gcgggctttg tcatccaacc gagcgccatt cagagtctcc tcgtagggtg
     5761 atcggtccca cgacgcgtat cccatcacag tgccagtcgg cccaaccgcg gagcgtaacg
     5821 cggcaacgac cgtctccgct cctccttcga ccggaccaat cgctttaagt gaggcatgca
     5881 ccatcaacag gtcaccggtt tggactccga gttttcgaat tgcctccgtt attgccttcc
     5941 gcgtatgcat cgcgatatct cctctaaact gcaaaacact atacggggtc tgacgctcag
     6001 tggaacgaa
//
LOCUS       DAOFQD010000200         5934 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.201, whole genome shotgun sequence.
ACCESSION   DAOFQD010000200 DAOFQD010000000
VERSION     DAOFQD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.201"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            144..1013
                     /locus_tag="QBF46_004517"
     CDS             144..1013
                     /locus_tag="QBF46_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDQ6676615.1"
                     /translation="MKATNVKSEKIHAPSVQESELVVVQNQSDEIPVLEWQGVRVVTT
                     ETLARGYGTETIRIRQNHHENKVRFVEGKHFFKVEGESLRELKHRVALNYSVKIARNV
                     RSLTLWTERGAARHAKMLETDQAWAFFEKLEDSYFRQKEQQPIAIPQTLPEALRLAAE
                     LAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVSVAKGILIGNFAKVVGLKQNALFVWL
                     RENGILIASGGRKNVPFQQYINAGYFTVKEVVLDDEDGYQIRLTPQLTGKGQQWLTRK
                     LLDAGLLKPVAAE"
     gene            complement(1046..1897)
                     /locus_tag="QBF46_004518"
     CDS             complement(1046..1897)
                     /locus_tag="QBF46_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676616.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDTASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DNPESAQ"
     gene            complement(2008..2217)
                     /locus_tag="QBF46_004519"
     CDS             complement(2008..2217)
                     /locus_tag="QBF46_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HDQ6676617.1"
                     /translation="MAFIQPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQLDSQ"
     gene            2822..3043
                     /locus_tag="QBF46_004520"
     CDS             2822..3043
                     /locus_tag="QBF46_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HDQ6676618.1"
                     /translation="MGYSAAKVSTHLELEKNRGYWRAKGFDRDSCQLSLSRGEEKIER
                     TRGRWRFYDENHKQVKAEPILYTLLKTII"
     gene            3051..4082
                     /locus_tag="QBF46_004521"
     CDS             3051..4082
                     /locus_tag="QBF46_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6676619.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVASPSPTSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEDGN"
     gene            4133..4444
                     /locus_tag="QBF46_004522"
     CDS             4133..4444
                     /locus_tag="QBF46_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HDQ6676620.1"
                     /translation="MRKDFNIDGKYVVLSVSTNILSPSVIVTVKLSDRMPDIDSISVA
                     FPVKSMRSAEHFVMNATEEEARRGLTRVMGEFGELLGKVNNALSISSARSKALTASMM
                     K"
     gene            4692..5252
                     /locus_tag="QBF46_004523"
     CDS             4692..5252
                     /locus_tag="QBF46_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /protein_id="HDQ6676621.1"
                     /translation="MKTIEQKLEQRREWQKAARERAIARQREKLADPAWRESQYQKMR
                     DSIDRRIAKQKERPPASKTRKSAVKIKSRGLKGRTPTAEERTIANALGTLPCIACYMH
                     GVISEEVSLHHISGRTAPGCHKKQLPLCRWHHQHAAPAEVREKYPWLVPVHADGVVGG
                     KKEFTLLNKSEMELLADAYEMANIMH"
     gene            5441..>5934
                     /locus_tag="QBF46_004524"
     CDS             5441..>5934
                     /locus_tag="QBF46_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HDQ6676622.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VASA"
BASE COUNT         1628 a         1314 c         1479 g         1513 t
ORIGIN      
        1 tgatcaccaa acaactttac cggagaaagg acaagaacgc ggttcaccgt atcctgttgt
       61 ctgatatccg cgaatacctg ctcgaacagg gtcatctgaa ttacgcaacc gccgcagccg
      121 aatcacgcaa ggaggcacac agaatgaaag caactaacgt taaatcagaa aaaattcatg
      181 caccttcagt tcaggaatcg gagctggtgg ttgttcagaa tcagtctgat gaaattcccg
      241 ttctggaatg gcagggagtg cgtgtagtga caaccgagac tcttgctaga gggtatggga
      301 cagaaacaat ccgtattcgc caaaatcatc atgagaacaa agtacgcttt gttgaaggga
      361 agcacttttt caaagttgaa ggagaatcat tgcgcgagtt gaagcacaga gtagctttaa
      421 actactctgt aaaaattgct cgcaatgttc gctcactcac cctctggaca gaacgcggcg
      481 cagcccgcca cgctaaaatg ctcgaaaccg atcaggcatg ggcattcttt gaaaaactgg
      541 aagacagcta cttccgacaa aaagaacagc aaccgatcgc aatcccccag acgcttcctg
      601 aagccctacg cctggctgcc gaactggctg aacaaaagca acttctggaa cagaaagccc
      661 accagctaaa tcagcagctg gtggccgccg ctcctaaagt cgattttgcc gaccgggtat
      721 cagtagctaa agggatcctg attgggaatt ttgcaaaggt tgttggactt aagcaaaacg
      781 cgctgtttgt ctggttacgg gagaacggca tcctgatagc gtccggtgga cgtaaaaatg
      841 tgccgttcca gcagtacatc aacgcggggt atttcacggt gaaagaagtg gtgctggatg
      901 atgaagatgg ctaccagata cggttgacgc ctcaattaac gggtaaaggc cagcagtggt
      961 tgacgcgtaa actgctcgat gctggcttgt taaaaccggt ggcggctgaa taatggaaga
     1021 atgcccggtt gatgccgggc ataatttatt gcgcgctttc ggggttgtcg tttactggct
     1081 gccccttctt ggttttacgg ctgcgcgtaa ctgatgcggc tgacttaacc tttttctctt
     1141 cgcgagtgat ggcaatttgt ttttttacat tttcaatatc tgccaggcga tatatttttg
     1201 cttgcggcca gcggtcgcag atgatcggtt ctatggagtc ataaaggcta aattttgctt
     1261 tttcgaattc accgttgatg ataattccat cacggagagt ttcatcgcag ataaacacgc
     1321 cacacagtgg cacatggtaa ctaactgatt taccatcatt gtagttaggg ctactggaaa
     1381 tgtagtggac gcgcagcatt gtttcgctaa agccgtgtac gcgcatacgg aatttttcat
     1441 cctccgggta ctgcttcatt agctcttttg ttgcttccag gttctctatg tatttcgcac
     1501 tgtgctcatt gatccccgcg cttttttgga tgcgaatgtc cttatcaatc agatgaataa
     1561 tgcggccagc ggtcatgttg acgctgttca cagcttctgt ctgataagtt gtaaccttac
     1621 gcacaccgcg aaggatgtta ggcactggat ataaaatagt ctttgggata ttgaggtctg
     1681 ggtactgttc cagttcccgc gccattaaag tccatttatc aatttcagcc tgaatgctgt
     1741 cagtttcttt gaacggtaga acgacaaccg ggcgtacagg acgaccgtcg ctggctgtat
     1801 caacgtgttg ggcgcgtgca acagcttttt ttagaaagag atccctgaag ctgacgaact
     1861 cctggtacag ttgttcgccg tagacataat ttatcattga tcctcctcca gaattgacat
     1921 ggtcaataac gcccggctga gaaaaccggt cattactgac ctatattata gagggatcaa
     1981 acaaaaataa tagatttatt agtgcattta ttgtgagtct aactggttag ttgccatgag
     2041 atattcgatt gtgtcagtga ggtcatccag gtcgtcttgg gtgatgcggt actcctgatt
     2101 ggatatcttt gagtagtgtt cagcaatggc gcgggcagcg tcggtttcgg cagggtctac
     2161 agataaagcg ttagagcaat gtctaacgtc gtcgatggtt ggttgaatga aagccataat
     2221 tatgtctcac tgtattgaca acacagagcc tgaagctctg acctactgtt tcacccatga
     2281 tccatgctgg ggtaatctaa caacattgcg ctgtgtgtaa gatgagcaat gcatagctgt
     2341 aatgccgttg tataaggttt ccctgtttgc tcatttcctt ctgagccgct ctacaacgct
     2401 gaagacacat taaatagtga atccaaagtc gtattacgaa acggcggcaa aactataatt
     2461 tattagagca attgtcaaac aactatgaaa aacaatccag tttttagctg gtggagtggg
     2521 atttttctct caaaatttat tgctctaata attcttgatt tttgtgcgca gctggacgta
     2581 aactcctctt ctgacctgat aacttcgtat agcatacatt atacgaagtt atcttaaggg
     2641 ttattgaaca tgatcaattc acctgtaaat ccatacagtt caatacctta tcaggtcaaa
     2701 tagtgatcac ttgatcattt gatcaaggtt gcgctacgta aaatctgcga aatgttggca
     2761 gtgttagtac tccagatttc gcgtagcgca cttagcacca ccaatcaatc agaggtgaaa
     2821 aatgggatat tcagctgcta aagtgtccac tcatcttgag cttgagaaaa accgtggtta
     2881 ctggcgggca aaagggtttg atcgtgatag ttgccaactg tcattatcgc gcggtgaaga
     2941 gaaaatagaa cgcacgcgcg gtcgctggcg tttctatgac gagaaccata aacaggtaaa
     3001 ggcagagccg atcctgtaca ctttacttaa aaccattatc tgagtgttaa atgtccaatt
     3061 tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc
     3121 gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa
     3181 tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt
     3241 ttcccgcaga acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg
     3301 cagtaaaaac tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc
     3361 tgccgcgacc aagtgatagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa
     3421 acgttgatgc cggtgaacgt gcaaaacagg cactagcgtt cgaacgcact gatttcgacc
     3481 aggttcgttc actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc
     3541 tggggattgc ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag
     3601 atatctcacg tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg
     3661 ttagcaccgc aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat
     3721 ggatttctgt ctctggtgtg gctgatgatc cgaataacta cctgttttgc cgggtcagaa
     3781 aaaatggtgt tgcctcgcca tcacccacca gccagctatc aactcgcgcc ctagaaggga
     3841 tttttgaagc aactcaccga ttgatttacg gggctaagga tgactctgga cagaggtacc
     3901 tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgtgccggag
     3961 tttcaatacc ggagatcatg caagctggtg gctggaccaa cgtaaatatt gtcatgaact
     4021 atatccgtaa cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcaatt
     4081 agccattaac gcgtaaatga ttgctctaat tctttgatat ttatggtggc atatgagaaa
     4141 ggatttcaac atcgacggaa aatatgtagt gctgtctgta agcactaata ttctgtcgcc
     4201 atctgtcatt gtcaccgtaa agttgagcga taggatgcct gatatcgact cgatatctgt
     4261 tgcgttcccc gttaaaagca tgcggagtgc tgaacatttt gtgatgaatg caacggagga
     4321 ggaagcgcgg cgcgggctta ctagagtgat gggggaattt ggcgaactcc tgggtaaggt
     4381 aaacaatgcc ctttcaatca gttcagcaag gtccaaagcg ttaacagctt ccatgatgaa
     4441 ataaaaaaaa gcctggcaag gagccaggct gcacaaaaga gcgggtttgt attccgcatc
     4501 caatcaatca agaaggagta tagcacacag gtactgaagt gaaaaaatgt gattcgcgag
     4561 aaacaaaata tctaccattg ctctaattga ttgctataat ttagccgcag tttttgtcaa
     4621 ctacgaagac gttgccatta cttcactcct tgacatcatt ggcggccatt aggccgcctt
     4681 ttttttgcca tatgaaaaca atcgaacaaa aacttgaaca gcgccgcgag tggcagaagg
     4741 cagccagaga acgagcgatc gctcggcaac gggaaaagtt ggctgacccc gcctggcgag
     4801 aatcgcaata tcagaaaatg cgggattcta tcgaccgccg tatcgctaaa cagaaagagc
     4861 gcccaccagc cagcaaaacg cggaaaagtg cggtaaaaat aaaatctcgt ggcttgaagg
     4921 ggcgaacacc gacggcggag gaacggacca tcgccaatgc tcttggcact ctcccctgca
     4981 ttgcctgcta catgcatgga gtaatatctg aagaggtgtc tctgcaccat atctccggtc
     5041 gtaccgcgcc gggttgtcac aaaaagcaat tgcccctttg cagatggcac caccagcatg
     5101 cagcaccggc tgaagtaaga gaaaaatacc cctggctggt ccctgttcat gccgatggcg
     5161 tggtaggagg caagaaagaa ttcaccttgt tgaacaagtc agagatggag ttgctggctg
     5221 acgcctatga aatggcaaac atcatgcact aataaatata ttatttttaa tcttaaatga
     5281 ttgacaactg acaagtgact tgagtcagaa tcatcacacg cccggtacgg atggatccct
     5341 tttcaaatat tccatggacg gcacagtctg agtaccgggc gctaccttca gttgtattgc
     5401 taagccgccg ctggtggctt ttcttttttg taggggcgct atggataaga aaatatgcgt
     5461 tgtttcaatg agcgtcggca aaccggcgtc aatgactgct gcatggatca acaatgagct
     5521 gataatggct gagcggacca gctaccctga acgccgccgc gatatggaac tccagctgct
     5581 gcgcgaattg cgagaaaaag aggaaaaggg ttttatcgtg ctggtggaag aggaaaacag
     5641 ctttattacc ggtcgagttg gccagcgtgt aaggttgcgt gatcccttca tgaacggcag
     5701 accggtactg attgaagcaa tgcagattta caaggaattg gaacgccaga aagcgatcaa
     5761 gttaccgcgc aaggaatccg gcaaatacat ccttcaccaa agcatcttcg attccgaaca
     5821 tgacaaaaaa ggcgatgaat ttttcaacat caactggagc gaaataacga cagagcacgt
     5881 tctgacgttg ttatgctgct tcgcaacgga atataacaac gttgcaagcg ccga
//
LOCUS       DAOFQD010000201         5866 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.202, whole genome shotgun sequence.
ACCESSION   DAOFQD010000201 DAOFQD010000000
VERSION     DAOFQD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.202"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            301..951
                     /gene="csgD"
                     /locus_tag="QBF46_004525"
     CDS             301..951
                     /gene="csgD"
                     /locus_tag="QBF46_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDQ6676623.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            956..1345
                     /gene="csgE"
                     /locus_tag="QBF46_004526"
     CDS             956..1345
                     /gene="csgE"
                     /locus_tag="QBF46_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDQ6676624.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1370..1786
                     /gene="csgF"
                     /locus_tag="QBF46_004527"
     CDS             1370..1786
                     /gene="csgF"
                     /locus_tag="QBF46_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDQ6676625.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1813..2646
                     /gene="csgG"
                     /locus_tag="QBF46_004528"
     CDS             1813..2646
                     /gene="csgG"
                     /locus_tag="QBF46_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDQ6676626.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2710..3201)
                     /locus_tag="QBF46_004529"
     CDS             complement(2710..3201)
                     /locus_tag="QBF46_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDQ6676627.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3303..3857)
                     /gene="ycdY"
                     /locus_tag="QBF46_004530"
     CDS             complement(3303..3857)
                     /gene="ycdY"
                     /locus_tag="QBF46_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDQ6676628.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPAHHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3881..4618)
                     /gene="ycdX"
                     /locus_tag="QBF46_004531"
     CDS             complement(3881..4618)
                     /gene="ycdX"
                     /locus_tag="QBF46_004531"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDQ6676629.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCREVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4673..5611)
                     /gene="ghrA"
                     /locus_tag="QBF46_004532"
     CDS             complement(4673..5611)
                     /gene="ghrA"
                     /locus_tag="QBF46_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDQ6676630.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         1603 a         1429 c         1331 g         1503 t
ORIGIN      
        1 ttttttaaaa ttgtgcaata aaaaccaaat gtacaacttt tctatcattt ctaaacttaa
       61 taaaacctta agattaacat tttaatataa cgagttacat ttagtcacat gtttaacact
      121 tgatttgaga tttgtaatgg ctagattgaa atcagatgta atccattagt tttatatttt
      181 acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca gtacttctgg
      241 tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg gggtttcatc
      301 atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac taaaccttct
      361 ttgcaggcga cagctctctt gcagcacctt aaacaatcgc tggcaattac aggaaaatta
      421 cataatattc aacgttctct ggacgatatc tcttcaggct ctattattct tctggatatg
      481 atggaagcgg ataaaaagct tatccattat tggcaggata ctttgagcag gaaaaacaac
      541 aatatcaaaa tattgttgct aaatacgcct gaagattacc cgtaccgcga cattgaaaac
      601 tggcctcata tcaacggcgt tttttattcc atggaggatc aagaacgtgt tgtcaatggg
      661 ttgcaaggcg tcctgcgcgg cgaatgctac tttacgcaaa agcttgccag ctacctgatt
      721 acgcattcag gtaactatcg ttataacagc acggaatcag ccctccttac tcatcgggaa
      781 aaagagatcc tgaataagct gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg
      841 ttcatcagcg aaaatacggt taaaacgcat ctttataatc ttttcaagaa gatagccgta
      901 aaaaaccgga cacaagcggt ttcctgggca aacgataacc tcaggcgata aagccatgaa
      961 acgttattta cgctggattg tggcggcaga atttctgttc gccgcaggga atcttcacgc
     1021 cgttgaggta gaagtcccgg gattgctaac tgaccatact gtttcatcta ttggccatga
     1081 tttttaccga gcctttagtg ataaatggga aagtgactat acgggtaact taacgattaa
     1141 tgaaaggccc agtgcacgat ggggaagctg gatcactata acggtcaatc aggacgttat
     1201 tttccagact tttttatttc cgttgaaaag agacttcgag aaaactgtcg tctttgcact
     1261 gattcaaact gaagaagcac taaatcgtcg ccagataaat caggcgttat taagtacggg
     1321 cgatttggcg catgatgaat tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa
     1381 acatgcagta gttctactca tgcttatttc gccattaagt tgggctggaa ccatgacttt
     1441 ccagttccgt aatccaaact ttggtggtaa cccaaataat ggcgcttttt tattaaatag
     1501 cgctcaggcc caaaactctt ataaagatcc gagctataac gatgactttg gtattgaaac
     1561 accctcagcg ttagataact ttactcaggc catccagtca caaattttag gtgggctact
     1621 gtcgaatatt aataccggta aaccgggccg catggtgacc aacgattata ttgtcgatat
     1681 tgccaaccgc gatggtcaat tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc
     1741 gaccatccag gtttcgggtt tacaaaataa ctcaaccgat ttttaagccc cagcttcata
     1801 aggaaaataa tcatgcagcg cttatttctt ttggttgccg tcatgttact gagcggatgc
     1861 ttaaccgccc cgcctaaaga agccgccaga ccgacattaa tgcctcgtgc tcagagctac
     1921 aaagatttga cccatctgcc agcgccgacg ggtaaaatct ttgtttcggt atacaacatt
     1981 caggacgaaa ccgggcaatt taaaccctac ccggcaagta acttctccac tgctgttccg
     2041 caaagcgcca cggcaatgct ggtcacggca ctgaaagatt ctcgctggtt tataccgctg
     2101 gagcgccagg gcttacaaaa cctgcttaac gagcgcaaga ttattcgtgc ggcacaagaa
     2161 aacggcacgg ttgccattaa taaccgaatc ccgctgcaat ctttaacggc ggcaaatatc
     2221 atggttgaag gttcgattat cggttatgaa agcaacgtca aatctggcgg ggttggggca
     2281 agatattttg gcatcggtgc cgacacgcaa taccagctcg atcagattgc cgtgaacctg
     2341 cgcgtcgtca atgtgagtac cggcgagatc ctttcttcgg tgaacaccag taagacgata
     2401 ctttcctatg aagttcaggc cggggttttc cgctttattg actaccagcg cttgcttgaa
     2461 ggggaagtgg gttacacctc gaacgaacct gttatgctgt gcctgatgtc ggctatcgaa
     2521 acaggggtca ttttcctgat taatgatggt atcgaccgtg gtctgtggga tttgcaaaat
     2581 aaagcagaac ggcagaatga cattctggtg aaataccgcc atatgtcggt tccaccggaa
     2641 tcctgacaga taaaaaagcc ggaattatca tccggcattt tactgtttaa gcgaaagcgt
     2701 gagtgcccct cacgctttat ttttgatttc ctgctcacgg tgcgccgtct ttgcactacg
     2761 cgccgccagc cacaggccac tgtttttcat tgcgtaacca aatatcagcc caagcgccag
     2821 cgaaggtaac accagtttcc agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa
     2881 cgttcccgga acaaatgaaa gcagcagctg tttggcctga atacacatca gaaacgcgac
     2941 aataccggtt atgacataac cgagaatttc cagatgtggt gccagtgcac taccgtaaat
     3001 aatgaccatc gcccacacca cgccactaag cagggttgca gcggagatcg ccagcccttt
     3061 cagcccacct tgcgggcaag caaagtaggc cgtacagccc aggaagcccg cccagcttag
     3121 taagcccagg gaaacagcca cccatcccca gataccggag agaatgcctg ttgtgattgc
     3181 aatagagaga agtatgttca tgccgcgcat gatagcagaa aacgcggcca tgaatgagat
     3241 cgagcacaca ttttatgcaa caacaaatta tctgttgcag ttatgagtga cgatgatcac
     3301 atttactctt cagaatcttc ttccagctcg tcccacattg cactaatggc atcacgggtt
     3361 agcggtgcca tggtgcgcca gaaaggcgtg gttgcatggg cctccacttt gccgaggaac
     3421 gcaccacacc agggtaacag atactcactg aacagtgttt ccagtgcttc gctctcatct
     3481 tccgttgact gatcttccag ccaggaagcc gcaagcagca atgtgccgat gtgatgggct
     3541 ggcgtatccg ccagtggcat ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt
     3601 gcgtcctcaa cccatgcgct acgatatggc ggcacggcac attcatcgcc gataaacaac
     3661 gcattgtaat cggcagagac ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac
     3721 tcatcctgct ccagtggcca gttcgcagcc agtttcccct cacgaatcag ggtaaacagc
     3781 ggcaccagta aaggatcttg cggttggcgg taatagagcg aacccagcac acgacagagg
     3841 atagaaaact cgttcattaa tatatttcca ttactagtaa ttaaaggtct gcaaattccg
     3901 caatcggtgc cataccgcga gattcaagga agttcagtaa gcggcgcgga gaaacattca
     3961 aaatgcgctc tggcggaaaa tctaccgcgt cgaggatttt aagacactct tcaaattccc
     4021 ccatggtaaa cgcggtgtga gaatccgagc ctaatgccac ccaacctcca gcatcacgta
     4081 ccgctgcggc tacctcacga cagttgtctt cactgccctt acgtgagtgt aaaaatgagg
     4141 aattattgat ttccagcgcc acctgatgtt tcgcggctgc ttcagcaacg gctttcacat
     4201 ctatttcata tttgggattt ccgggatggc ttattatatg cacattgccg ctggcgatag
     4261 tggcgatcat cgcttgtgta ttggtcgctt tgtcatgtgg cgcaaaaacc ggctcatgaa
     4321 aaccggcaat aattagatcc agcgagtcaa acattttacc gctgcagtca atttcaccat
     4381 caacattttt aatgttagct tcgatgccgc gcaggatccc taccccatca accactcgcg
     4441 gccagatacg catgttaatg aagtgccagt gatgcggcgc atcttccata tccgggccat
     4501 gatcggtgat cgcaaaaagt ttaatgccct tttgtttggc ctgggcaatg taatcactta
     4561 atgtgctata tgcatgtgtg ctggcaacgg tatgcatatg aaggtcgacg ggatacataa
     4621 ctctctcctg tattcaattc ccgccaagga tagcaggaat cctgatgctt tattagtagc
     4681 cgcgtgcgcg gtcgacttgc ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc
     4741 gagaaatgta ctccacagct tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg
     4801 tcacgcgtgg atgttgccag agcggacttt caggcggtaa gggttcacga ttaaaaacat
     4861 ccaacattgc gcctttaact ttgccgctat ccagcgccgc gagcaggtca tcttccacaa
     4921 catgaacacc acgcgccagg ttgaggagat acgcgccatc cggtaatttt tcgagtaatt
     4981 gttgattaat aatgccgacg gtttcagggg tattcggtaa caaattaatc aatacccgac
     5041 attggctcag aaatgcagac agttcttccc gtccggcaaa gctttgcacg ccaggccacg
     5101 atttacgggt tcgactccag caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa
     5161 ctttactgcc cagtacgcct gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt
     5221 caggcagcgg ttgccaatgc gaactatttt gctggatgcg ataatcgtca aaacgtcgaa
     5281 accaatgcag cacctgactg acagcatatt cctgcatttg ctcgcccata ccggtatctt
     5341 ccaggcgaaa aagtggaaca gaagggttca gcatttcagg gtgtgcctgt agcttgctca
     5401 aaatagaatc aacaccggcc ccgagtgcga acaccgcttt aagatcgcgc cctgccagca
     5461 tttcaacagg aggatgccag actaaagcat aatcagcaga gtcattatct ccgcttttcc
     5521 atgctctgac tcttgcctga ggaatagctt tgcgcagtgc ctcaatccac cattgggtat
     5581 cgaacgttgg gtgataaaag atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat
     5641 ttattcgcac ttatcgttat gatctacaaa ggccaccagc ataacaaatc cgtggtcggt
     5701 ggcaaaaaaa gcagatttcg cttattaaaa ctgcatattg attgaagttt gaataaacgc
     5761 gcgatttttt aaaaaagttt gttgacctca ggtcatgatt tccctaaatt agcgcccgtt
     5821 ccagcaagac aggaacgaca atttggtgag gtgtccgagt ggctga
//
LOCUS       DAOFQD010000202         5845 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.203, whole genome shotgun sequence.
ACCESSION   DAOFQD010000202 DAOFQD010000000
VERSION     DAOFQD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.203"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1176)
                     /gene="thrC"
                     /locus_tag="QBF46_004533"
     CDS             complement(<1..1176)
                     /gene="thrC"
                     /locus_tag="QBF46_004533"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDQ6676631.1"
                     /translation="MKLYNLKDHSEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     "
     gene            complement(1177..2109)
                     /gene="thrB"
                     /locus_tag="QBF46_004534"
     CDS             complement(1177..2109)
                     /gene="thrB"
                     /locus_tag="QBF46_004534"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDQ6676632.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2111..4573)
                     /gene="thrA"
                     /locus_tag="QBF46_004535"
     CDS             complement(2111..4573)
                     /gene="thrA"
                     /locus_tag="QBF46_004535"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDQ6676633.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRICGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSFIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRSLELADIEIESVLPAEFNAEGDVATFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDVDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(4599..4718)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(4654..4719)
                     /gene="thrL"
                     /locus_tag="QBF46_004536"
     CDS             complement(4654..4719)
                     /gene="thrL"
                     /locus_tag="QBF46_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDQ6676634.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(4933..5619)
                     /locus_tag="QBF46_004537"
     CDS             complement(4933..5619)
                     /locus_tag="QBF46_004537"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDQ6676635.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         1395 a         1626 c         1421 g         1403 t
ORIGIN      
        1 ttcaccgaga atcgcttcca cgctctcttt aaatttcgcc ggatgcgcgg tgccgaggaa
       61 caagccatat tcgcctggat tcaactggtc acgcagcgca cgatacgcta cggcagcgtg
      121 cggctccgag gtgtagccca gttcttttaa ctcacgcatt gtctgttgcg tggtttcatc
      181 atccacggct gcataaccca gctctttcag ttgccagatt ttgcggcgga acaactcttc
      241 cacacgcggc cagttgtttg gctgactcac gtccatcgcg ttggataacg tcgcctgagt
      301 cgctttgggt gaccactgac catcgtgcag gaaacgtggc acggtatcgt tcacgttggt
      361 cgcagcaata aaacgtttca ccggcagacc gagtgacttc gccagcagac ccgccgtcaa
      421 atcgccgaag tttccgcttg gcaccgagac aaccagctgg ttgcgcgctt cctgcggcag
      481 ctgcgcaaca gcttcaaagt agtagcaaat ctgcgccagc aaccggctga tgttaatcga
      541 gttagctgag tttaacccca gcgccacttt cagctcttca tcatcaaacg cctgcttcac
      601 cagcgcctga caggcatcga aatcgccgtc gatggcaaca gtttcgatat tgccgcccaa
      661 tgtacagaac agtttttctt gcagtggact gattttgcct cgtggataga ggataaccac
      721 tttcacattc ggtaaaccgt agaaagcatg agccactgcc gctccggtat caccggaggt
      781 cgcggtcaga atggtcactg gcttatcgcc cgcaatatgg gtcagcattt gtgccataaa
      841 gcgaccgccg aaatctttaa atgccagcgt tggcccgtgg aacaattcca gacaaccgac
      901 atcgctttca acattggcga ccggagccgg gaaggcaaac gccgcgcgca cgcgctcttc
      961 caggatttcc tgcgggattt catcaccaat aaacgccgag aggatcttcg cactgcgggt
     1021 gacaaaatcc agcttcagca tctcatcaat ttcagtcagg ctgaattccg gcaggtcatg
     1081 cggaaaaaac agcccctgat ttttgcccaa cccctgggtt acggcttgcg caaagctgac
     1141 ctgctcgctg tgatctttca gattgtagag tttcatttag ttttccagta ctcgtgcgcc
     1201 cgccgtatcc agccggcaaa tatgaacaaa accttcctga ttttgcaggt agttcttacc
     1261 caaccagtcg gcaacgcgct gggcggtatc cggcttgtca cacagagcga acaaggtcgg
     1321 gccggagccg gagataccgc tcgctaccgc gccgatttcc gcgaccgcct gccgcgcctg
     1381 ccggaagcca ggcagtaacc gttcacggta gggttctgcg ataacatctt tcatcagctt
     1441 cgcggcaagc tcaggctgac gggaatagca ggcgtgaatg aagcctgcca gatgtcgccc
     1501 gtgcgcaatg caatcctggc ggcgatactg cgccggtaaa atagcccggg cttctgccgt
     1561 cgagacttta atccccggat acgccagcac ccacagccac tcatcaaacc ctggcacttg
     1621 ctggctgatg atgtcgtttt cttcgatcat caactgcata ccaccaagaa aacacggtgc
     1681 cacgttgtcg taatgaatgc tgccggagat tcgtccttcc agctcgccca tcaaagccag
     1741 caaacgagtg tcattaagcg gcttgccaca gtgttcattc atcgccatca gcgccgcgac
     1801 caccgaacag gcgctggagc ctaagcccga gccgatcggc atattctttt ccagagtcat
     1861 cgccactgga atttgcttgc caagctcctg gcaaaaacgc tcccagcact gataaacgat
     1921 attttcccgt ggttctgacg gcagcttatc ggcaaagcgt ccgaggttgt tgagactgaa
     1981 tgtctctgcc gcctcaaccg tgactacatc tccgagcaat gcaccatcaa cgggtgtcac
     2041 cgccgccccg agcacatcaa acccgacgct catattggca ctggaagccg gggcataaac
     2101 cttaaccatt ttagactcct aacttccatg agagggtgcg tagcagatcg gcaaagacac
     2161 cggcagctgt aacgtcatta cccgcaccgt atccgcgcag gaccagcggc agtggctgat
     2221 aatagtggct atagaaggcc aatgcgtttt cgccgttttt cactttgaac agcggatcgt
     2281 tgccatccac ttcagcaatc ttcacgcggc agacgccatc tacatcaata ttgccaacat
     2341 agcgcaaaac ttttccttca tcacgggcct tcgccacgcg cgcggcaaag agatcgtcga
     2401 gctgtgacag attcgccata aaagtcgcaa catcgccttc cgcgttaaac tctgcgggca
     2461 gcacagattc gatttcgata tccgccagtt ccagtgatcg tcctgtttca cgggcgagaa
     2521 tcaacagctt gcgcgccaca tccataccag aaagatcatc tcgcgggtcc ggttcggtat
     2581 agcccatttc tcttgccagc gtggtggcct cggagaagct catcccttcg tctaacttac
     2641 cgaagatgaa agaaagcgaa ccagaaagaa tgccagagaa tttcatcaat tcatcacccg
     2701 cgttgagcag gttttgcaag ttctcaatga ccggcaaccc cgcaccgacg ttggtgtcat
     2761 aaaggaattt acgccgtgat ttttccgccg cgtaacgcag ctgatggtag taatccatcg
     2821 acgaggtgtt ggcctttttg ttcggggtca ccacatggaa accttcgcgc aggaagtcgg
     2881 catattgatc cgccactgcc tggctggagg tgcagtcaac aatgaccggg tttagcagat
     2941 gatattcttt cacgaggcga attaagcgcc cgagattaaa cggctctttg gcttgcgcca
     3001 gttcttcctg ccagttttcc aggtttaggc catgcacatt ggtaagcaga gccttcgagt
     3061 tggcaacgcc gcagatacgt aagtcgatat gtttattctt cagccagctt tgttgacgct
     3121 tcagttgctc cagcagcgca ccgccaacgc caccgacgcc aatcacaaat acttcgataa
     3181 cctgatcggt attgaacagc atctgatgag taacgcgcac gccagtggtc gcatcatcgt
     3241 tatttaccac gacagagatt gagcgttcag aagatccctg agcaatggcg acaatgttga
     3301 tattggcgcg ggccagcgcg gcaaagaatt tcgccgagat cccacgcaag gtgcgcatac
     3361 catcacctac caccgagata atggccagcc gttccgtcac cgccagcggc tccagtaagc
     3421 cttctttcag ttccaggtag aactcttcct gcattgcccg ttcagctcgc acacagtcgc
     3481 tttgcggaac gcagaaactg atactgtatt cggaagatga ttgcgtaatc agcaccacgg
     3541 aaatacgggc gcgtgacatc gctgcaaaca cgcgcgccgc catgccaacc atccctttca
     3601 tccccgggcc ggaaacgctg aacattgcca tattattcag attggaaatg cccttgaccg
     3661 gtaattcgtc ttcatcacgg ctggcaccaa tgagcgtacc tggagcttga ggatttccgg
     3721 tatttttaat caggcaaggg atctggaact gggcgatggg ggtaatggtg cgggggtgaa
     3781 gaactttagc gccgaagtag gaaagctcca tcgcttcctg gtaggacatc gacttcaaca
     3841 acctcgcatc gggcacctga cgcgggtcgc aggtatagac cccgtcaacg tccgtccaaa
     3901 tctcgcaaca atcggcgcgt aaacaggcag ccagcaccgc cgcggagtag tcggaaccgt
     3961 tgcgtccaag taccaccagt tcgccttttt cattaccggc ggtgaaacct gccatcagca
     4021 ccatgtgatc agccggaatg cgacttgccg caatacggcg ggtggactca gcaatatcga
     4081 cggtagattc gaggtaatgc cccactgcca gcagtttttc gaccggatcg ataacggtaa
     4141 cgttgtgacc gcgcgcttct aatacgccgg ccataatggc gatcgacatt ttctcgccac
     4201 ggcaaatcag cgcagcgttg atgctatccg ggcactgccc caacaaacta atgccatgca
     4261 ggacatgttt tatttgggca aattcctgat cgacgaaagt tttcaattgc gccagcggga
     4321 atcccggctg ggcggcggcg agtcccgtca aaagttcggc aaaaatacgt tcggcatcgc
     4381 tgatattggg taaagcatcc tggccgctaa tggttttttc aatcatcgcc accaggtggt
     4441 tggtgatttt ggcgggggca gagaggacgg tggccacctg cccctgcctg gcattgcttt
     4501 ccagaatatc ggcaacccgc agaaaacgtt ctgcatttgc cactgatgta ccgccgaact
     4561 tcaacactcg catggttgtt acctcgttac ctttggtcga aaaaaaagcc cgcactgtca
     4621 ggtgcgggct tttttctgtg tttcctgtac gcgtcagccc gcaccgttac ctgtggtaat
     4681 ggtgatggtg gtggtaatgg tggtgctaat gcgtttcatg gatgttgtgt actctgtaat
     4741 ttttatctgt ctgtgcgcta tgcctatatt ggttaaagta tttagtgacc taagtcaata
     4801 aaattttaat ttactcacgg caggtaacca gttcagaagc tgctatcaga cactcttttt
     4861 ttaatccaca cagagacata ttgcccgttg cagtcagaat gaaaagctga aaaatactta
     4921 ctaaggcgtt ttttatttgg tgatattttt ttcaatatca tgcagcaaac ggtgcaacat
     4981 tgccgtgtct cgttgctcta aaagccccag gcgttgctgt aaccagtcga ccagttttat
     5041 gtcatctgcc actgccagag tcgtcagcaa tgccatgact cgttcgcgta aagcttgcag
     5101 ttgatgttgg tctgccgttg tgtcactttt cgtcggttgt tgtattaatg ttgctaattg
     5161 atagcaatag accatcactg cctgccccag attgagcgaa ggataatccg ccaccatcgg
     5221 cacaccagta agaacgtcag ccaacgctaa ctcttcgtta gtcaacccgg aatcttcgcg
     5281 accaaacacc agcgcggcat ggctcatcca tgaagatttt tcctctaaca gcggcaccag
     5341 ttcaactggc gtggcgtagt aatgatattt cgcccgactg cgcgcagtgg tggcgacagt
     5401 gaaatcgaca tcgtgtaacg attcaaccag tgtcgggaaa actttaatat tatcaataat
     5461 atcaccagat ccatgtgcga cccagcgagt ggctggctcc agatgtgctt gactatcgac
     5521 aatccgcagt tcgctaaacc ccatcgtttt cattgcccgc gccgctgccc caatattttc
     5581 tgctctggcg ggtgcgacca gaataatcgt tatacgcata ttgccactct tcttgaccaa
     5641 atgaccgcga acagggtgat cactgtcaac ttattacgcg gtgcgaattt acaaatcctt
     5701 aacgtaagtc gcagaaaaag ccctttactt agcttaaaaa aggctaaact atttcctgac
     5761 tgtactaacg gttgaattgt taaaaaatgc tacatatcct tctgtttact taggataatt
     5821 ttataaaaaa taaatctcga caatt
//
LOCUS       DAOFQD010000203         5794 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.204, whole genome shotgun sequence.
ACCESSION   DAOFQD010000203 DAOFQD010000000
VERSION     DAOFQD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.204"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            496..909
                     /gene="hns"
                     /locus_tag="QBF46_004538"
     CDS             496..909
                     /gene="hns"
                     /locus_tag="QBF46_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDQ6676636.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(1054..1962)
                     /gene="galU"
                     /locus_tag="QBF46_004539"
     CDS             complement(1054..1962)
                     /gene="galU"
                     /locus_tag="QBF46_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDQ6676637.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(2164..3177)
                     /gene="rssB"
                     /locus_tag="QBF46_004540"
     CDS             complement(2164..3177)
                     /gene="rssB"
                     /locus_tag="QBF46_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDQ6676638.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(3269..4174)
                     /gene="rssA"
                     /locus_tag="QBF46_004541"
     CDS             complement(3269..4174)
                     /gene="rssA"
                     /locus_tag="QBF46_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDQ6676639.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            4287..4745
                     /locus_tag="QBF46_004542"
     CDS             4287..4745
                     /locus_tag="QBF46_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDQ6676640.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            4795..5637
                     /gene="purU"
                     /locus_tag="QBF46_004543"
     CDS             4795..5637
                     /gene="purU"
                     /locus_tag="QBF46_004543"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDQ6676641.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         1563 a         1435 c         1277 g         1519 t
ORIGIN      
        1 acgaagcctt gcataatcct tctgagctat cattacaaca gcctcgcttg ttataagcgg
       61 gtaaatgact gctggtaact attcacaatc tttaacctgt tgcgcaagta atagccctct
      121 gttgacctcc aggagatagt gcaatactaa gtccctgctc ttattgcgac tgttctactt
      181 ttcatcattc gcttaatagg gaattctcgt aaacacgact aatacagaag actgaaaggt
      241 cgtcagccta cgataatctc cccataaaat gtgacatgaa tcaggaagtt ttaacctcac
      301 gtgctgcgaa atcatcggtg caaatagggc tatatgccgc gtcttttctg gctaatttta
      361 tgaaaagata tttattggcg gcacaaaata aagaacaatt ttgaattcct tacattcctg
      421 gctattgcac aactgaattt aaggctctat tattacctca acaaaccacc ccaatataag
      481 tttgagatta ctacaatgag cgaagcactt aaaattctga acaacatccg tactcttcgt
      541 gcgcaggcaa gagaatgtac acttgaaacg ctggaagaaa tgctggaaaa attagaagtt
      601 gttgttaacg aacgtcgcga agaagaaagc gcggctgctg ctgaagttga agagcgcact
      661 cgtaaactgc agcaatatcg cgaaatgctg atcgctgacg gtattgaccc gaacgaactg
      721 ctgaatagcc ttgctgccgt taaatctggc accaaagcta aacgtgctca gcgtccggca
      781 aaatatagct acgttgacga aaacggcgaa actaaaacct ggactggcca gggccgtact
      841 ccagctgtaa tcaaaaaagc aatggatgag caaggtaaat ccctcgacga tttcctgatc
      901 aagcaataat cttttgtaga ttgcacttgc ttaaaatccc gccatcggcg ggatttttta
      961 ttgtccggtt taagacaatt taataagaat aagctaataa aaaaacggcg tcgattgctc
     1021 aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca tctcttcttc
     1081 aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt caacgaaggc
     1141 ctgcatgtaa cctaatttat taccgcagtc atgactcttc cctttcatat gataggcttc
     1201 caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt catcaccagc
     1261 tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt aacgacccac
     1321 aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac caaccatcgg
     1381 tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat atgcggtcac
     1441 atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc ggcggatcat
     1501 ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa catcaggcaa
     1561 aataacagct accggttcat cacccactac cgggtgagca cacaataccg cgtgtcccag
     1621 gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac aaatagactg
     1681 cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca gttcaaaact
     1741 ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca caatttcagt
     1801 aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat cgacaagtgg
     1861 cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta atcccgcaac
     1921 ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc tcctggactg
     1981 ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag caccccgaag
     2041 gggctaagtt ctgaaaagga ggcgagttag cataattaag acaattactt atacgaatgg
     2101 cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg cgttatcgtt
     2161 tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata tttggcattg
     2221 ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag tgcctaacgg
     2281 aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat tcagacccgc
     2341 agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata gcggaaactg
     2401 ccccggcaga ttggcctgac ggagtaaatg gtttacctgc ttcaataacg cgcccaactc
     2461 tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa acaatgcgcg
     2521 taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa ggcaataaaa
     2581 tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt tgtccgcggc
     2641 aaccaattga cgataattaa cccggcaatg ggaaatcacc tgctgaaccg gcggttgtag
     2701 ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc agtcgcgaaa
     2761 aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac aggcaaaaac
     2821 catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt caacgcccag
     2881 acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca gaactggggt
     2941 ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc gtggcatcgc
     3001 gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg catccacccc
     3061 atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca gaagcgagcg
     3121 aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct gcgtcatgtt
     3181 ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg cctgtcagaa
     3241 ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac ggcaaaagtt
     3301 cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc gcgcgatgga
     3361 aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc ggatcacctg
     3421 ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc ataatctctg
     3481 ttgccgttgg cgctgtcacc gcccgacgtg tcgttatgct gcctaacctt tctttcagac
     3541 gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca ttaaaggaga
     3601 gcaaatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc acaatatcag
     3661 cacccaatgc acgcgtgagg gaaataggaa ttgggttaac gactgcccca tcaaccagcc
     3721 agtagccgtt atgcgcgact ggtgccatga gtcctggaat actgcatgat gcgcgaatag
     3781 caagatggag atcgccttca gtaaaccata attcacgccc agtacttaaa ttggtggcaa
     3841 cagccgcgaa gcgacgggaa caattttcga tctctgtttc cggcattatt tcgcggtatt
     3901 gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagaga tccatcaggc
     3961 gtaaaacatc ccaataactg aaagaggtca cccaatcttc cagcgcagat aatcgatcgc
     4021 atgcataggc agcgcccacc agcgaaccaa ttgaacatcc tgcaacgata tcaatttcaa
     4081 tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc gccgcgccag
     4141 atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc ccctggaatg
     4201 caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg gttaatacgc
     4261 cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt agtgctgtcg
     4321 agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct gatccagaac
     4381 atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgcagattat ttaataaaga
     4441 cctggcatcc atcttgtgga gcagcggcgt tacgtgccga attgattgcc ggatttgcgc
     4501 atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc gacaatatcg
     4561 gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca ataattgaac
     4621 gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca cgtccgcagt
     4681 ttggccgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag tgctgcggcc
     4741 aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc agcaatgcat
     4801 tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct gatcgcacgt
     4861 attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga atttgttgat
     4921 caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa tgattccacc
     4981 ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct gaatcctgcc
     5041 ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg cgatttgttg
     5101 atgaaagcca attacggcgg cctggatgtc gaaatcgcgg cagttattgg taaccacgat
     5161 actctacgtt ctctggttga gcgttttgat attccgtttg agctggtaag ccatgaaggg
     5221 ttaagccgca acgagcacga tcaaaagatg gcggatgcca ttgatgctta tcaacctgac
     5281 tacgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc acgcttcccg
     5341 aataagatca tcaatattca ccattcattc ctgccagcgt ttattggcgc acgtccttat
     5401 caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta tgtgaatgac
     5461 aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca tacctacaca
     5521 gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag tcgtgcacta
     5581 tacaaagtac tggcacagcg cgtctttgtt tacggtaatc ggacgattat tctttaatcg
     5641 ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc aaccagttca
     5701 tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc cccgcttccc
     5761 gataagggag caggccagta aaaagcatta cccc
//
LOCUS       DAOFQD010000204         5781 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.205, whole genome shotgun sequence.
ACCESSION   DAOFQD010000204 DAOFQD010000000
VERSION     DAOFQD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.205"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(109..2892)
                     /locus_tag="QBF46_004544"
     CDS             complement(109..2892)
                     /locus_tag="QBF46_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDQ6676642.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2949..5315)
                     /locus_tag="QBF46_004545"
                     /pseudo
     CDS             complement(2949..5315)
                     /locus_tag="QBF46_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(5353..>5781)
                     /locus_tag="QBF46_004546"
     CDS             complement(5353..>5781)
                     /locus_tag="QBF46_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDQ6676643.1"
                     /translation="FKGDISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSE
                     VLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQE
                     AIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1456 a         1342 c         1306 g         1677 t
ORIGIN      
        1 atcactatta gtacttgatt attattttga acgcatttat aaaattatta cataaaaata
       61 gcgaatattg ctaaaatccc cgccaacgat gtgttgacgg ggctgttatt attttggcaa
      121 taatactccg gtataagtat ttaccggatg agaaagatat tgtttaacgg cagtgttaac
      181 attctctacc gtcatttgtt tcaacaattg ctcctgctca gtccatgctg caggatcgtc
      241 atattgaata agactattta caatagtgtt cgctaattgt tgaacgctac gctgttggat
      301 atcgaggctg cgctgaacgt tttgctggta ttcattcagt tcttgctcac tgatcccttt
      361 agccagacgc ttaaccatca cttcattcgc taacgttaac agttcatcat gtcgttctgg
      421 ttgacaagta aaagccagca aatgactgat atctttggcc tgaggatcaa ccgagaggcg
      481 agaagaaacg ctgtatgctc cagatgcctg ttcacgaata ttaacacgta gatcttttgc
      541 cagtgcgacg ttaaaagcat cgagcgccat acgcgtcgcc agattaacag gtgtccggga
      601 atcataacgc ttccactgtg aaacctgtgc cacaggttca ttttgttctt ttacagtaac
      661 cgatgcgttg tccgtcgcgc gagttaatgg tttacctgcg gctaatggcg aatcagagtg
      721 tttgattgat cctaagtaac gcgtaattaa cgccacgagt ttgtcttctg agacattacc
      781 gacaatgaca aacgtgatat ccgctggaga tgaaaacaac tggcgatcgg cagccagcgc
      841 atctgcggca gtaaactgta caatctgatt ttcttgcagt aatttcgtgc ggtcatcagc
      901 atagcgcgtc tcatacatct gctgggcgaa tttctccgct ggacgctggt cgagcgtttt
      961 caacgcctga atttgagcat tttgtagcga tgcccaaata ttgtcgttaa tcgtgctgtg
     1021 ggtgattcgc tggttaatca actgaaaacc aggttcaggg ttattagtcc gcgcgctaac
     1081 ggagagcaac gtattcatgc cactgacttt actgctcatg gttaccgaat tttccgcact
     1141 ccagcgtttc aggctggaag aggagagctc accaacgccg cttccgctta ctgctttatt
     1201 tgccagcgcg ataagtgatt tttgctgcgc agggaaactt aaatcgcctt tattcgatac
     1261 ggcagtaatt tgtagctttt gctcttcacc cgcggatttt gccagaataa ccctggcacc
     1321 attggaaagt gttaatgatg tcagattctc agccagcgtt tctttgctgc taatttccgc
     1381 ctgtgggtca gcgtctactg ttaacgataa atttctgcct gggaagatgt aagccgccag
     1441 ctttttgttg gcgtactctt tttccagcgc caggatagcc gcaggagaca atgctttttt
     1501 ggcggcaagc tcattgttta ccatttgctc ccaaaatgcg tcctggttct ttcttaactg
     1561 ctgccatttt tccgccagac tttgcacggt aatttgctgc cacagacgtt tcgaaagttg
     1621 atatgtctct tccggcgaca agaacggcgt attatttaat gagctggatg ccaggcgact
     1681 ggtcagcata cgtaaatcac gttcagcttg ctgatcaacc gcatttttca gccaggtgag
     1741 gcgggtagat ttgacatcat cgagttcttc agcagaaaaa ccatgctgat caatggttgc
     1801 caactctgcc attaatgcat tcgcagcatc ctgcatatta tcgtctcgtg catttacacg
     1861 gaaaaacagc gactgataat cgggtgcaat tttaacgctg cgcgcagtgc cgccagaaat
     1921 agtcttcaac tcgcccgact gtatgcgttc ctgcagacgt tgattgaaca gctgaactaa
     1981 catgctccat tcagcttgtt cgacaaagct ttgctcatcg ttcacttgta ccattggcag
     2041 gcgataatag agtgcgatgc cgttcacccg gttttcttta tcattaatga tattaaagcg
     2101 caggtggttt tcggcttttg tcggccagac gcgattttca gctgctttgt tagccggaag
     2161 cttacttaaa ttatccttta tcagcgccag cgcttcttta ctgtcgatat cgccgaccac
     2221 gataaaggtc atattatttg gttgatacca gcgttgataa aattggcgca attgtgccgg
     2281 tgtgaccgtg gcgacagtat ccatcaggcc gataggttca cggtctaaat tacgggtatt
     2341 tgccagcagg aaagggcggc gcgcctgaga ggtgcgccat ttcgcatcct gatgggcacg
     2401 ccattcctca gtaattacgc cacgttcagc gtctacttcg agtttttcaa aggttgcggc
     2461 attactccat tcactgaaga ttgccatcac ttgttgcaga ttttgtttct gcgtagtcgg
     2521 caaactcacc tgatacaccg tttcgtcata gctggtatag gcattaacat cgcgaccaaa
     2581 acgcaggccc attgactcaa atgtttcgat gactttatta cccggccatg tttttgtgcc
     2641 gttaaacatc atatgttcta caaaatgagc cacgccgcgc tcattgtctt cttcctgcaa
     2701 tgaaccggta tgaatttgca gccataaatt tacctgatcc tttggatgag catgcggata
     2761 aatcatatat cgcaagccat tgtccagttg cccggtaatt aacttttcat cctgaggtaa
     2821 ggcggcggca atcagccgcc cggggagcaa cagagttgcc actaacgtca gtaagaaaca
     2881 gaggtttctc ataattatct ccatgcgaaa accgggcgaa tttacccggt taagtaaaat
     2941 ccgaactatt aaaatttcat gctgacatca agccagaaag tacggccact ggcgtaggtc
     3001 gccacaccgg tgttcgttgt atcaacagcg gttttgctat ccagtacatt gagaatatcg
     3061 gcactgatcg tcaggttttg ttgtttcaga aattgtggcg tccaggacaa gcgggtgtcc
     3121 caggtcaggc tgctatccag tttttcgtca acatactact tgtaatctga atattcgctg
     3181 atgtattgcg catttgtctt accaaggata atgcgagctt tacgcgcttc ttgccaggcc
     3241 agcgtattcg cccacactaa cccgctcggt tgatgcgtga aatccatgtt taaggagatc
     3301 tttaatgggt tattaaaatc tgccactgga acgctatcgt aagagaccag attaccgtta
     3361 taaaccacct ggttatcacc ggtattgctc tcctcataac cattgtttaa cgacaagttg
     3421 cccttgctct tgatatagct aaagacaatt tgtgggttaa tatctacctg gcggatatgc
     3481 aggggttcgg ccagttcaaa actgaggctg aacgaatgcg ttttggtttt gccatcgttg
     3541 ttatattcag taatggtggt tttagtcgcg ctgtcggtac gactgctttt gctgatttga
     3601 tcatgcgctt cacggtaaac atagtttgca cgcgcaataa cgttcttacc gattttctgc
     3661 tgcaatccca ttgccagttc atcgttataa ggcgttttca aatcctgata acgcgtcaga
     3721 gttttattac ctgataccga ttccgtccag ctattgcgga tatcacgtaa tcccatatca
     3781 agaatattcc cgccatagta acggttataa cctgctgtaa tcattgaggt ttgattagca
     3841 aaaatatccc attccgtcat aaagcgcggg gagatattgt ggtttgacag atagttgtca
     3901 tagtcatacc gcacgccggg cattaatgac acattacgcc agctaatgcg atccgccata
     3961 tacagcgtgt agttgtcaat tcccaggcgg cctttacctt tatggtaaat ggtatggtta
     4021 gttttctttc cggcagcatt aatcacatag gattcagact ggttatggcg ttcagtccac
     4081 gcatcggaat agatgtattc cgcgccgaag tagggttgat gcgaaacatt acccacggcg
     4141 aatttttgcc agtccaggcg tgttttgaag gtgtaattat ctacagcctg ggaaatgtgt
     4201 cctaatccgc cacgggtgca acgccctgta atatcaccat atgtacatga aagttcggtg
     4261 taccagatgt catgatcgtg acgggtataa tcactaatat gatcccaacc aacggtggta
     4321 cgcagtttgg cccatgcgag ctgcgtatcc atatcccatg ccagaccata ggatttatta
     4381 cccatttcgc gatcagactg cgggaaggtg ctggtattat aatcacggct ggagccggta
     4441 tattttaagg ttaaatcgtg ggtaaagcgg tcgctggcaa accaggtaaa tttgctcaat
     4501 gcagtatcga taacgttttt atactgtgcc cgaccggcga caatgccgtc attcgaaaca
     4561 taatccgcgc gggtgatatc agactggcgg cgcgataaac cggcggtaac gccgaagtta
     4621 tcagcgagtt cctgattaaa cgacaaggta taaaagttct ttttaaaatc tggggagtaa
     4681 taagtacttc ctgaagagcc ttgattaaat gcgctcttgt tattctcatc gatatgcgat
     4741 gttaaccagt ccgaacgcgt agtgcgataa cccaatttca ccttgctatc atcagcgttg
     4801 aagcgtttga tctttgcatc aattaccccg ccattgaagc gaccaaattc aaccggcaca
     4861 aaactgtcat aaagcgtcac attgtccagt aagctgacat caagataata cccctgtgac
     4921 atcccgctaa tattggttgc actactggca tcggactcat tcgctgggtt caggttatta
     4981 gttgcactaa taccgtcaat caaataggca ttctggtagg gcgacgcacc gtgaatagag
     5041 attttctcag ggcgaatatc tccctggttc aacgaggtac tttgcgttga atccatgcgt
     5101 accgcagggt tggttctcag cagatcgctg atattgccat tcccggtcgg taaacgctcg
     5161 atgctttcac tggtgtaatg cgtgttgccg tttacgggta caggagcata gaccgtcatc
     5221 tcttcaaaaa aatgcatgta catgtttttg tcatcgacgg cctgcgccac atcagcgcca
     5281 cataaaatga cgccaggaat aagaactcgc ttcatttatt tatatctccg tacagcaggg
     5341 cttttattgt tgttataaaa ccgtgctaat gtcacaaata tcatcagcca ggttccagac
     5401 accgggttga tgtgtaacca taatgacgcc gcttgtgggt agtttttcac gcactaaacg
     5461 cagtaagcgt atagcctctt gttcctcaag atgagaggta gtttcgtcaa gaaatatcca
     5521 tttcggacgt cgtaaaatta atcgtgccag ggcgatacgt tgtttttcgc cgctggaaag
     5581 aatatctccc cagcgatcgt ggtcatgaat acgcgcagcc aatttcccca gaccaacctg
     5641 atgcagtact tcgctcaacg atttatcgtc tacgggcagg ggaagtgctt tacaaataat
     5701 ctctttcagt aagccgcttt tgattaacgg tgtttgtgac acataccaac tgtcagcagg
     5761 agaagaaata tcacctttaa a
//
LOCUS       DAOFQD010000205         5718 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.206, whole genome shotgun sequence.
ACCESSION   DAOFQD010000205 DAOFQD010000000
VERSION     DAOFQD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.206"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            314..469
                     /locus_tag="QBF46_004547"
     CDS             314..469
                     /locus_tag="QBF46_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HDQ6676644.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(466..954)
                     /locus_tag="QBF46_004548"
     CDS             complement(466..954)
                     /locus_tag="QBF46_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein B"
                     /protein_id="HDQ6676645.1"
                     /translation="MLDVFTPLLKLFANEPLERLMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            1396..1617
                     /gene="kilR"
                     /locus_tag="QBF46_004549"
     CDS             1396..1617
                     /gene="kilR"
                     /locus_tag="QBF46_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HDQ6676646.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            1617..1787
                     /locus_tag="QBF46_004550"
     CDS             1617..1787
                     /locus_tag="QBF46_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676647.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            1862..2137
                     /locus_tag="QBF46_004551"
     CDS             1862..2137
                     /locus_tag="QBF46_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HDQ6676648.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            2239..4839
                     /gene="recE"
                     /locus_tag="QBF46_004552"
     CDS             2239..4839
                     /gene="recE"
                     /locus_tag="QBF46_004552"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HDQ6676649.1"
                     /translation="MSTKPLFLLRKAKKSSSEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDDDKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGISKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNAGKEEE
                     KAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLSDCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            4832..5641
                     /gene="recT"
                     /locus_tag="QBF46_004553"
     CDS             4832..5641
                     /gene="recT"
                     /locus_tag="QBF46_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HDQ6676650.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNSDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
BASE COUNT         1753 a         1272 c         1369 g         1324 t
ORIGIN      
        1 atacctacca acccaccccg ccccacagaa cgcagggcaa tacttcgagt taccaggcag
       61 tggtaagggg ttaagtagcc agcccgaggc gtatgaacat gacggcggga ttcaaatttt
      121 gcagtgcagc agttagttcc gccacccggc gttaagggga gagataagat ggtgcattac
      181 gaagtagttc agtatttgat ggattgttgc ggtatcactt acaaccaggc tgtgcaggct
      241 ttacgcagca acgactggga tctctggcag gcagaagtcg ctatacgtag caacaagatg
      301 tgagattcgc aaaatgcaaa aaatcgacct cggcaacaac gaatccctgg tgtgcggcgt
      361 gttccccaac caggatggaa cgttcactgc catgacgtat accaaaagca aaacatttaa
      421 aaccgaaact ggtgcgcgcc gatggttgga gaagcacaca gtaagctaac gattaaaacg
      481 tctactcctg ctgttccaga ataacttcat aaaatgggag tatttttcgg tgacgagata
      541 ataagaacag tttgcgctat cactctgatg ttgaatgatg cccttccgtt ctaatttttt
      601 cataaccggg ttacggcaag gagaagtgat aataagattt cctgttttaa ggaaatcttt
      661 aaatacagcg atttctttct cagataaacg aagcaatact cgttgctctg gtagtaatga
      721 ataatgcttt tgaatatgtg ctcgcaatct tgagaaggaa atggcgacca cgaaagaaaa
      781 ggcaaaaacg ataatctgaa agagccaagg tatttcagta taagcattga atgcgacagt
      841 aaactctttc ggtatcagcc agagagtgag accaaaaatg ataatcgtat acataagtct
      901 ttcgagtggc tcgttagcaa aaagtttcaa caatggagta aatacatcca acatatcaat
      961 aactctcaac tgtaagggta ttgaaatgtt aacacaagct ctcgctgtag gggtatagcc
     1021 gagaccaccg aagcccggag gtggtgaaat aaaaccgggc acaacacgaa ggcgcatttc
     1081 cgatatccat aaagagtcgg tcttgtctgt taaatttaaa tggtgggagt gcgcctccgg
     1141 ttgtaaataa cgacattgct gtgtgtagtc ctggcggcat cagttttttt cttgaagttc
     1201 ggctgatgtc cgcccttttt aaagtgaatt ttgtgatgcg gtgaatgcgg ctaagcgcac
     1261 gtggcacagt taaaagtcat gttagtcctt attggtttgg gtgggaaagc cgactgtaat
     1321 tgttaactgg ttgcagtcac ctggaggcac caggcaccgc atcaacaaag ttcatttgta
     1381 aaaatggaga taattatgat tgcacatcac ttcggaactg atgaaatacc acgtcagtgt
     1441 gtgactcctg gcgattatgt tcttcatgaa ggccggacat atattgcctc ggcaaacaat
     1501 attaaaaagc gaaaactata tattcgtaac ctgaccacaa aaacatgcat tactgaccgc
     1561 atgattaaag tcttcctcgg tcgtgatggt ttacctgtaa aggcggagtc atggtgatga
     1621 ctaagaaaat aaaatgtgct taccaccttt gcaaaaaaga cgttgaagaa agcaaagcta
     1681 ttgaaagaat gcttcacttc atgcacggga ttttatcaaa agacgaaccg agaaaatatt
     1741 gcagtgaagc ttgtgccgaa aaagaccaga tggcacatga actttaatta attgactatt
     1801 cgaaactgaa tttatgccag aaatggcagg tattcgctca accttaatta aggagaaaaa
     1861 catgattacc aattatgaag ccactgttgt aactaccgat gacattgttc acgaggtgaa
     1921 tctggaagga aagcgcattg gctacgtaat taaaacagaa aataaagaaa ccccattcac
     1981 tgtggttgat atcgatggtc catcaggcaa cgtaaaaaca cttgatgaag gtgtcaaaaa
     2041 aatgtgcctg gtgcatatcg gaaagaatct gcccgcagaa aaaaaagccg aatttctggc
     2101 aactctaatt gcaatgaaat taaaaggtga aatctgaaag aaatagcctg cgtatggcgc
     2161 aggctatgaa cagtgtgtat ccggcaagat cattcactga acaaaacgaa ttttaatctg
     2221 agttgaggtt aaaaaacaat gagcacaaaa ccactcttcc tgttacggaa agcgaaaaaa
     2281 tcatccagtg aacctgacgt cgtcctgtgg gcaagcaacg attttgaatc gacctgtgcc
     2341 actctggact acctgatcgt taagtcaggt aaaaaactga gcagctattt taaagctgtt
     2401 gccacgaatt ttcctgtcgt taatgacctg cccgctgaag gtgagatcga ttttacctgg
     2461 agtgaacgct atcaactcag caaagactcc atgacatggg aactaaaacc gggagcagca
     2521 ccagacaacg ctcactatca aggcaatacc aacgtcaacg gcgaagacat gactgagatt
     2581 gaggagaata tgctactccc aatttctggc caggaactgc ccattcgttg gcttgctcaa
     2641 cacggcagcg aaaaaccggt aacgcacgtt tcacgcgacg gactccaggc attacacatt
     2701 gctcgggctg aagaactacc ggctgttact gccctggctg tttcccacaa aaccagcctg
     2761 ctcgacccgc tggaaattcg cgaactccac aaactggttc gtgacactga caaagttttc
     2821 cctaatcctg gtaattcaaa cctgggactg ataactgctt ttttcgaagc atacctgaac
     2881 gctgactaca ccgatcgagg actgctgaca aaagagtgga tgaagggtaa tcgtgtttca
     2941 cacatcactc gcacggcttc cggtgctaat gctggcggcg gaaacctcac cgatcgcggc
     3001 gaaggtttcg tacacgatct gacgtcactg gcgcgcgacg tagccactgg cgtactggcc
     3061 cgttcaatgg atctggacat ctataacctt catccggcac acgctaaacg cattgaggaa
     3121 attatcgctg aaaataaacc gcccttttct gttttccgcg acaaattcat caccatgcct
     3181 ggcgggctgg attattcccg cgccatcgtg gttgcgtccg taaaagaagc accaattggg
     3241 atcgaggtca tccccgcgca cgtcactgaa tatctgaaca aagtactgac tgaaaccgat
     3301 catgccaacc ctgatccgga aatcgtggat attgcctgcg gtcgctcctc tgccccgatg
     3361 ccgcagcgag taacagaaga aggaaaacag gatgatgaag aaaaaccgca accatctgga
     3421 acaacggcag ttgaacaggg agaggctgaa acaatggaac cggacgcaac tgaacatcat
     3481 caggacacgc agccgctgga tgctcagtca caggtaaatt ctgttgatgc gaaatatcag
     3541 gaactgcggg cagaactcca tgaagcccgg aaaaacattc catcaaaaaa tcctgtcgat
     3601 gacgataaat tgcttgctgc atcacgtggt gaatttgttg acggaattag cgacccgaac
     3661 gatccgaaat gggtaaaggg gatccagact cgcgattgtg tgtaccagaa ccagccagaa
     3721 acggaaaaaa ccagcccaga tatgaatcaa cctgagccag tagtgcaaca ggaaccggaa
     3781 atagcctgca atgcctgcgg ccagactggc ggggataact gccctgactg tggtgcggtg
     3841 atgggcgacg caacatacca ggaaacattc gatgaagaga gtcaggttga agctaaggaa
     3901 aatgatccgg aggaaatgga aggcgctgaa catccgcaca atgagaatgc tggcagcgat
     3961 ccgcatcgcg attgcagtga tgaaactggc gaagtcgcag atcccgtaat cgtagaagac
     4021 atagagccag gtatttatta cggaatttcg aatgagaatt accacgcggg tcccggtatc
     4081 agtaagtctc agctcgatga cattgctgat actccggcac tatatttgtg gcgtaaaaat
     4141 gcccccgtgg acaccacaaa gacaaaaacg ctcgatttag gaactgcttt ccactgccgg
     4201 gtacttgaac cggaagaatt cagtaaccgc tttatcgtag cacctgaatt taaccgccgt
     4261 acaaacgccg gaaaagaaga agagaaagcg tttctgatgg aatgcgcaag cacaggaaaa
     4321 acggttatca ctgcggaaga aggccggaaa attgaactca tgtatcaaag cgttatggct
     4381 ttgccgctgg ggcaatggct tgttgaaagc gccggacacg ctgaatcatc aatttactgg
     4441 gaagatcctg aaacaggaat tttgtgtcgg tgccgtccgg acaaaattat ccctgaattt
     4501 cactggatca tggacgtgaa aactacggcg gatattcaac gattcaaaac cgcttattac
     4561 gactaccgct atcacgttca ggatgcattc tacagtgacg gttatgaagc acagtttgga
     4621 gtgcagccaa ctttcgtttt tctggttgcc agcacaacta ttgaatgcgg acgttatccg
     4681 gttgaaattt tcatgatggg cgaagaagca aaactggcag gtcaacagga atatcaccgc
     4741 aatctgcgaa ccctgtctga ctgcctgaat accgatgaat ggccagctat taagacatta
     4801 tcactgcccc gctgggctaa ggaatatgca aatgactaag caaccaccaa tcgcaaaagc
     4861 cgatctgcaa aaaactcagg gaaaccgtgc accagcagca gttaaaaata gcgacgtgat
     4921 tagttttatt aaccagccat caatgaaaga gcaactggca gcagctcttc cacgccatat
     4981 gacggctgaa cgtatgatcc gtatcgccac cacagaaatt cgtaaagttc cggcgttagg
     5041 aaactgtgac actatgagtt ttgtcagtgc gatcgtacag tgttcacagc tcggacttga
     5101 gccaggtagc gccctcggtc atgcatattt actgcctttt ggtaataaaa acgaaaagag
     5161 cggtaaaaag aacgttcagc taatcattgg ctatcgcggc atgattgatc tggctcgccg
     5221 ttctggtcaa atcgccagcc tgtcagcccg tgttgtccgt gaaggtgacg agtttagctt
     5281 cgaatttggc cttgatgaaa agttaataca ccgcccggga gaaaacgaag atgccccggt
     5341 tacccacgtc tatgctgtcg caagactgaa agacggaggt actcagtttg aagttatgac
     5401 gcgcaaacag attgagctgg tgcgcagcct gagtaaagct ggtaataacg ggccgtgggt
     5461 aactcactgg gaagaaatgg caaagaaaac ggctattcgt cgcctgttca aatatctgcc
     5521 cgtatcaatt gagatccagc gtgcagtatc aatggatgaa aaggaaccac tgacaatcga
     5581 tcctgcagat tcctctgtat taaccgggga atacagtgta atcgataatt cagaggaata
     5641 attcagcctg gcggtgtaat gcaccgccaa cttgaaatat tttttatgag aaaaattatg
     5701 agatatgaca atgttaaa
//
LOCUS       DAOFQD010000206         5710 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.207, whole genome shotgun sequence.
ACCESSION   DAOFQD010000206 DAOFQD010000000
VERSION     DAOFQD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.207"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            85..519
                     /locus_tag="QBF46_004554"
     CDS             85..519
                     /locus_tag="QBF46_004554"
                     /inference="COORDINATES: protein motif:HMM:NF012982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HDQ6676651.1"
                     /translation="MSKQYHALIPGHIYCGGVNDIQQMVDEDGIEVVVDLRAESTGCA
                     AHQPEVQWVHISLSDHAEEPEEQLFHKAIFAVVDAYQKGKKVAFHCGAGRGRTGTVAA
                     GVLLELKICKSLPEAVELAKSKRPVLNIKPIQYEALTLLYPA"
     gene            complement(639..1220)
                     /locus_tag="QBF46_004555"
     CDS             complement(639..1220)
                     /locus_tag="QBF46_004555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676652.1"
                     /translation="MCEVEELRRTCDIAGSLLLKSPEQELIDWIKQCTGQNLAIKECQ
                     QAFYDYFCFPQSGLFCPPYNHVFSNGLRHGDIWQFPAASFDGGAEEEDFYQKFNFAPS
                     MLNIKLPMVHKHVPGDHFGVELLFISCVLTNENLNTPDTWSILKGFICAHLPGLKGYT
                     HLLGSQTDPYIKVVAECLEECLSGLDYLLSIDS"
     gene            complement(1230..4256)
                     /locus_tag="QBF46_004556"
     CDS             complement(1230..4256)
                     /locus_tag="QBF46_004556"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012602.1,HMM:NF013717.1,HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDQ6676653.1"
                     /translation="MNRRHFLTGCAALGVGVGASIYGKVFYRAGTFSDAGEQAKSTFY
                     GQSTEPEWLLDKETGQHVINPNYMVRHSVCLQCHGECGLRAKVNRKTGKLERLQGNPY
                     HPNTLIDYKDMNIPVVDTANTPGTLCARGNAGLQTAYDPYRLTVPLKRSGPRGSNKWV
                     PIEWEKLIDEVVNGGKIFADTGDEASQNLEIKGFRALYEKRDEWIDDNNHDLGRVSNK
                     LVIQSGRIVKTRKDFQTRFAKSFGTVNNYEHTNICELSHHIATSAVYTGHNNFKADLC
                     ESEFVLYWGTAPGEDNFPMQTLGKYSAEARSKGCKIAVIDPVLPRTITDDPNMFWLAP
                     RPGTDGAIAMGMISWIIEHNRHNTDFLSYPNQSSAKNAGETCFTDASYLIITDETHPN
                     FGKFITSSEAALGDSKDSVVINLSGIPELAEKCVKAMLDYSGNVNGIGVATAFHLLKT
                     SASDYSLEYYADISGVRVDDIVNLADEFTKHGRKASTEFYRGIAQHPNGYYTGFAINQ
                     LNVLIGNLNWSGGGSLGGGGFPYDKGVYDLTAIPSLVPAQFGIKITREDGFRYEDSTE
                     FKTKVARGEPPYPAKRPWFPFTKDIFSDILPSALEGYPYTADILLWHMCTPLYSAPGM
                     GKEDIIRGICDPTKIPLIIASDIVVSDTSMYADYIIPDITYLERYVQHPMLEATMVKG
                     TAVRYPVIEPLTGKNQAGQHFCLETFFIDIASKLNMAGFGKNAIPDSNGTLWPLEKME
                     DYYLKGTANVAYSGTPVSDATEDDIKIAGLEDYQKKYSSSLKSDEWRKVLFLMSRGGR
                     FEPVTTRRNGTQLKNQFNNRIRMYNEKIAQGLNSFTGERFSGTALWQPSLTMMGKKVD
                     ELDQGKGMDFTILTRKSALQTQSRLSSNSFIREIQPTNWAEINTEDGKALGLKTGDSV
                     WVETVEGRRRCEVKLRDGVAPGVISFIVGYGHWGYGATDIDIGGKRIKGSKIRAAGIN
                     LNPIMRLDPDVWGMPLMDPIGGSSSFFNTRAKIIKA"
     gene            complement(4253..5260)
                     /locus_tag="QBF46_004557"
     CDS             complement(4253..5260)
                     /locus_tag="QBF46_004557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676654.1"
                     /translation="MMTTILNIAHQSPFGLLFINYSLLLGASGGLAVIWSFLNWGSQS
                     KTNLLPVALAFALAFGGILNVLAEVQQPGRLIYGFIYGWEHWYGSIIKYGVLILPLFC
                     LLLILQLTPLKLSVFVDKLTKILLIGTGIFLGMYSGIFMTNEHGIALWNTPLSAVLML
                     LSGLYTGFIAYSLVSKHSNEQPAVISSYCGLGVMLITMFILLLFTWWGHVFAGETISA
                     SFGLLDSNYLATQIITVGALVLACVMISVIKSKNKLHYTIIIILSAISAWGVRFLLLV
                     GGQGTSRSSAGVFTYHPEPKEYLYSVGSIFFMAGILALVILLYENFQHKYPAHTGGEI
                     K"
     gene            complement(5264..>5710)
                     /locus_tag="QBF46_004558"
     CDS             complement(5264..>5710)
                     /locus_tag="QBF46_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDQ6676655.1"
                     /translation="CVPVCPTGATFKRNDGIVLINQDICWGCGACVTACPYDARFINT
                     ETKTADKCTFCAHRIEQGLVPACVETCVGAARVFGDLNDEESEVHKLYSDHITNVLNP
                     ATGNKPQVFYIGLNNEMTVGEDIKSKTWTKEFSDVFDQELPWVNQE"
BASE COUNT         1726 a         1273 c         1069 g         1642 t
ORIGIN      
        1 atcatattta aagtcaaaca ggtttgtttg gaatgtttag caggtaaaga attcaccaat
       61 atttttcagg agctaaaaaa gtaaatgagt aagcaatatc acgccctaat acctgggcat
      121 atctactgcg gtggtgtaaa tgatatccag caaatggtag atgaagatgg gatcgaagtg
      181 gtggttgatt tacgtgctga atcgacgggt tgtgctgcac atcaaccaga ggtgcaatgg
      241 gtgcatatat cattaagtga tcatgctgaa gagcctgagg agcaactgtt tcataaagcg
      301 atttttgctg tagtagatgc ctatcaaaag gggaaaaagg ttgcatttca ttgtggtgca
      361 ggcagggggc gaacaggaac tgtagcagca ggagttttac tggaattgaa gatctgcaaa
      421 tctttaccag aggccgtaga actggccaaa tcaaaacgtc cagtattgaa tattaaacca
      481 attcaatatg aggcattaac tctcctctat cctgcctgac taaattatga atcgccacag
      541 aatactaggt aatgtgccag ccaggtaagc actgagagcc agattaatat tttagtctgg
      601 ctctatttct gaaaatatct atgtagttac attttgattc aagaatcaat cgatagtaaa
      661 taatcaagtc ctgataaaca ctcctccaga cattcagcta caactttaat ataaggatca
      721 gtttggctgc ctaataaatg agtataccct ttcagaccag gtaagtgagc acatataaat
      781 ccctttaaaa tgctccaggt atcgggggtg tttaaatttt catttgtcag cacacaactt
      841 ataaaaagca attccacacc aaaatggtca ccaggaacat gtttatgtac catcggcaat
      901 ttgatgttaa gcatagaggg agcaaaattg aatttttgat agaagtcttc ttcttctgct
      961 cccccatcaa agcttgctgc aggaaattgc cagatgtctc catgacgtaa gccattactg
     1021 aagacatgat tatagggcgg acaaaacaaa ccagattgtg ggaagcagaa atagtcataa
     1081 aacgcctgct gacattcttt aatcgcaagg ttttgccctg tacattgctt tatccagtca
     1141 ataagctctt gttcagggct tttgagtagt aaggatccag caatatcaca ggttcttctc
     1201 aactcttcca cttcacacat acttgccccc tacgctttga taattttagc tcgtgtatta
     1261 aagaaactgc tactgccacc aattggatcc ataagcggca tcccccatac atctggatcc
     1321 agtcgcataa ttggatttaa atttatcccc gcagcccgga ttttactgcc tttaattcgc
     1381 ttcccaccaa tatcaatatc agttgctccg tagccccaat ggccatagcc aacgataaag
     1441 ctgatcactc cgggtgcaac accatctctg agctttactt cacaacgcct gcgcccttca
     1501 acagtttcga cccatacact atcaccggtt ttcaaaccaa gagctttccc atcctcggta
     1561 ttaatttcag cccagtttgt cggctgtatc tctcggataa aactgttgga agacaatcgg
     1621 gactgggtct gaagtgcact ttttcgggtc aatattgtaa aatccatacc tttgccctga
     1681 tcgagctcat ccactttttt ccccatcata gtgagggatg gttgccaaag tgcagttcct
     1741 gaaaaacgtt caccggtaaa actatttaga ccctgagcaa ttttttcatt gtacattctg
     1801 atacggttat tgaattgatt tttcaattgc gtaccattcc gtctggtagt cactggctca
     1861 aaacgaccac cgcgtgacat taaaaatagt acttttctcc attcatcaga cttaagacta
     1921 ctggaatatt tcttctggta atcttcaaga ccggcaattt ttatatcatc ctcagtagca
     1981 tctgatactg gtgttccact ataagcaacg tttgcagtac ctttcagata gtaatcctcc
     2041 attttttcga gaggccatag agttccatta ctgtcaggaa tagcattttt cccaaaccca
     2101 gccatgttta atttcgaagc tatatcaata aagaaagtct caagacaaaa atgttgtccc
     2161 gcctgattct ttccagtcaa cggctcgata acgggataac gtacagcagt ccccttgacc
     2221 attgttgctt ccagcatcgg atgctggaca taacgctcca gatatgtaat gtcagggata
     2281 atataatctg catacatgct ggtatcacta acgacaatat cactggcaat aattaatggt
     2341 atttttgttg ggtcacaaat ccctcgaata atatcttctt tacccatgcc tggagcgctg
     2401 tataaaggtg tacacatatg ccacaacaga atgtcggctg tatacggata accttccagt
     2461 gctgaaggca agatatcact gaaaatatct ttagtgaaag ggaaccacgg ccttttagct
     2521 gggtaggggg gttcgccacg agcaaccttt gtcttaaatt cagtagagtc ttcataacga
     2581 aaaccatctt cacgtgtaat ttttatacca aattgagctg gcaccaaaga aggaatagca
     2641 gtcaggtcat atactccctt gtcataaggg aatccacctc ccccgaggga accaccacca
     2701 ctccagttca aattaccgat taagacgttc aactgattta ttgcaaaacc tgtatagtaa
     2761 ccgttcgggt gctgtgcaat tccacgataa aactctgtgg atgctttccg accatgttta
     2821 gtgaactcat cagctaaatt gacaatatcg tcaacgcgaa caccactgat atcggcataa
     2881 tactctagag aataatcact cgctgatgtc tttaataaat gaaatgctgt agcaactcct
     2941 atgccattaa catttccgct ataatccagc attgctttaa cacatttttc cgctaattcc
     3001 ggaatgccag agagattaat tacgacactg tctttactgt cccccaaggc agcttcacta
     3061 gatgtaataa atttcccaaa attagggtgt gtttcatcgg taataatcag gtaactggca
     3121 tccgtgaaac aagtttcacc tgcatttttg gcagaagatt gatttggata acttaaaaaa
     3181 tccgtattat ggcgattatg ttctatgatc cagcttatca ttcccattgc gatagcacca
     3241 tctgtgcctg gtcgcggagc aagccagaac atatttgggt catccgttat agttcggggt
     3301 aatactggat caataacagc aattttgcag ccctttgaac gcgcctctgc tgagtatttc
     3361 cctaatgtct gcatagggaa attatcttct ccaggcgcgg ttccccaata caaaacaaac
     3421 tctgactcac aaagatctgc tttaaaatta ttatgcccag tatatacagc actcgtagca
     3481 atgtgatggc ttaattcgca tatattggtg tgttcgtaat tattaacagt gccaaaggat
     3541 ttcgcgaagc gcgtctgaaa atctttacgc gttttaacaa tacgtccgct ttgtataaca
     3601 agcttattag atactcgccc taagtcatga ttgttatcat caatccattc atcacgtttt
     3661 tcgtataatg cccggaatcc ttttatctca aggttttgac tagcctcatc acctgtatca
     3721 gcgaaaatct ttccaccatt gaccacttca tcgatcagtt tttcccactc aataggtacc
     3781 catttattac ttcctctcgg gccagagcgt ttcaggggaa ctgtcagtcg atatgggtcg
     3841 taagctgttt gtagacccgc gttccctcgg gcacacagtg taccaggggt attggctgtg
     3901 tccaccaccg ggatattcat atccttatag tcaatcaatg tgttcggatg atatggatta
     3961 ccctgtaagc gttccagttt tccggtttta cgattcactt tggctctcag tccacactct
     4021 ccatgacact gaagacatac agaatgacgt accatataat tgggatttat aacatgctgg
     4081 ccagtttctt tatcaagcaa ccattcgggt tcagttgact gaccataaaa ggttgatttt
     4141 gcttgctctc ctgcatctga gaatgtacct gcacggtaaa atactttgcc ataaatactg
     4201 gcaccaacac ctacacctag cgcggcacaa cctgtaagaa aatgacgtct gttcatttaa
     4261 tttctcctcc tgtatgtgcc ggatatttgt gttggaaatt ctcataaagc aatatgacta
     4321 aagcaagaat acctgccata aaaaatatac taccaacaga atataaatat tcttttggtt
     4381 cggggtgata agtaaagact ccggcactgc tacgactcgt tccctgcccc ccaaccaaca
     4441 aaagaaaacg aacaccccag gcagagatag cagatagaat aatgataatt gtgtagtgga
     4501 gtttattttt agatttaatt acagaaatca taacgcaagc taaaactaac gctcccacag
     4561 taattatctg cgttgctaaa tagttagaat caagtaaacc aaaggaggcg ctaatagttt
     4621 ctcccgcaaa gacatgcccc caccaggtga atagcaataa gataaacata gtaataagca
     4681 tcacaccaag cccgcaataa gatgaaatta ctgccggttg ttcattacta tgtttagaaa
     4741 ctaaactgta ggctataaat ccagtataaa gtccactaag taacattaag actgcagaca
     4801 gtggtgtatt ccagagtgca ataccatgtt cattagtcat gaatattcca gagtacatcc
     4861 ctaaaaatat cccagttcct ataagaagga ttttcgttaa tttatcaaca aaaacagaca
     4921 gctttaatgg cgtgagttgc aggattagta gtaggcaaaa caggggtaaa atcagtacgc
     4981 catattttat tattgaaccg taccagtgtt cccaaccata tataaaccca taaattaacc
     5041 ggcctggttg ttgtacctct gctaatacat ttaatatacc tccaaacgct aatgcaaaag
     5101 ccaatgctac aggtaaaagg ttagtctttg actgactccc ccagttaagg aaggaccaga
     5161 taacagcgag acctcctgat gcacctaaca ataacgagta attaataaaa agcaagccaa
     5221 atggagattg atgtgcaata tttaaaatgg tcgtcatcat gatttactcc tgatttaccc
     5281 aggggagttc ctgatcgaaa acatcagaaa actctttcgt ccaagtctta ctttttatgt
     5341 cttctcctac agtcatttca ttgtttaacc caatataaaa tacctgaggc ttgttacccg
     5401 ttgctggatt caaaacattt gttatatgat ctgaatataa tttatgcact tcactttctt
     5461 cgtcattcag atcaccaaaa acccttgctg caccgacaca agtttcgaca caggcaggga
     5521 cgaggccctg ttctatacga tgcgcgcaaa aggtacattt atcagctgtt ttggtttcag
     5581 tattaataaa acgagcatca taagggcagg cagtaacaca ggcaccacag ccccaacaaa
     5641 tatcctgatt gataagaaca atgccatcat tgcgtttaaa cgtagcacct gttggacaaa
     5701 cagggacaca
//
LOCUS       DAOFQD010000207         5703 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.208, whole genome shotgun sequence.
ACCESSION   DAOFQD010000207 DAOFQD010000000
VERSION     DAOFQD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.208"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..693)
                     /locus_tag="QBF46_004559"
     CDS             complement(<1..693)
                     /locus_tag="QBF46_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676656.1"
                     /translation="MLIQKKRIRNIDNYIPNFDGRDIYIAHSLPDAGKTDSIGFSSQR
                     ALGEEVLPKILGPITRFNANGKSVPDKTAPKETAYRQISWTRKQWIGGGATQEVTDTR
                     EIEYKRYKRILTPPPSIELKIVENLQGEKLIISPKLSLTANNKELVAHCINLFLELFG
                     LCETVDDQLNTIVKSKSIKLNWSLLPQGQQPWSTIGSKILSAIKIRGKGNTAVVEGRL
                     ELINSKKPDFVAV"
     gene            1669..2229
                     /locus_tag="QBF46_004560"
     CDS             1669..2229
                     /locus_tag="QBF46_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6676657.1"
                     /translation="MATFGYGRVSTSQQDTENQRLELGQAGWQIDYWFADVVSGKVPA
                     MQRKAFSEMLNKIRDGETLVVAKLDRLGRDAIDVLQTVRALADRNIKVLVHQLGNTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTQAGLSRAKAEGKKLGRPPKIASEARREILEK
                     KNSGLSISALARQYGVSRATVAAITD"
     gene            2243..3088
                     /locus_tag="QBF46_004561"
     CDS             2243..3088
                     /locus_tag="QBF46_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676658.1"
                     /translation="MAYTATVIPVMIASPGDVAEERQVIREMIHEWNDINSARSKVML
                     TPIGWETHTSPELGVRPQKLINQRLLVDCDLLIGVFWTRLGSPTGNEASGTVEEIHRH
                     LNAGKPAMIYFSSKPVAPESLDREQYESLKLFKTECMQKGLIESFNDLSDFKDKVRRQ
                     LSIIISSSPYLSSLISTINNSPDANTSQSLPESNLSADALSLLKLACVDDSGTIYVIR
                     HLGGTDIQAGNQSFGGSSAREVARWEGALNELLSFDYVIERGAKGQMYYVTHKGWTFL
                     ESLNE"
     gene            complement(3149..3643)
                     /locus_tag="QBF46_004562"
     CDS             complement(3149..3643)
                     /locus_tag="QBF46_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676659.1"
                     /translation="MTGTDFLLPIAAGAAISWASYSVVAHAVSKQHISTLRATVWGAL
                     GTLSLVLGVCAFSAVGAEHQTARKDIVCADDNPECYAKTHHNPVRECKKVMDEKAKFR
                     HVWQESEAPVFNTYLWYDENAKIIQAFGQQAKVVNGLGMLAPLQYFCVFNAKTGEVIA
                     ASFE"
     gene            3884..4348
                     /locus_tag="QBF46_004563"
     CDS             3884..4348
                     /locus_tag="QBF46_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6676660.1"
                     /translation="MKKSLRIQFGERVKELRIATGLSQEAFADRCGFARSYMSRIERG
                     GSNASLDAIEVLANALSVEPWQLLAPGPSEDNDSELLVPYAADGSCFHPGLASTRDGS
                     FGVGDKAAQKRFGTFAEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            complement(5088..>5703)
                     /locus_tag="QBF46_004564"
     CDS             complement(5088..>5703)
                     /locus_tag="QBF46_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDQ6676661.1"
                     /translation="IRLPAERKIQDYRSAYNDIRDWQRREKEADKKEKSTTDWDDVVF
                     EIDLLKSQEINLDYILGLIFEHNRQNKGKGEMTEEVKRLIRSSLGNRAKEGLVVDFIQ
                     QTNLDDLPDKASIIDAFFTFAQREQQREAAALIKEENLNEEAAKRYIRTSLKREYATE
                     NGTELNETLPKLSPLNPQYKTKKQTVFQKIVAFIEKFKGVGGQI"
BASE COUNT         1471 a         1240 c         1321 g         1671 t
ORIGIN      
        1 aaccgctacg aaatctggtt ttttactatt gataagttca agtctgcctt caacaacagc
       61 ggtattgccc tttcccctga tcttaatagc tgaaaggatt tttgagccga tagtactcca
      121 aggctgttga ccctgaggca ggaggctcca gtttagtttt atagatttac ttttaacgat
      181 ggtattaagt tggtcgtcaa cagtttcgca caacccaaaa agctctaaaa aaagattaat
      241 gcagtgagcc actaactctt tgttgttagc tgtcaaactt agctttggag agatgatcaa
      301 cttctcccct tgaagattct ctacaatctt taactctatg cttggtggag gggtgaggat
      361 tctcttgtag cgcttatact caatttcacg agtgtcagta acttcttgag ttgcgccccc
      421 tccgatccat tgctttctag tccatgaaat ttgtctgtat gcagtttctt taggtgctgt
      481 tttgtcaggt actgatttgc cattagcatt gaatctggtt attggcccta aaatcttggg
      541 gaggacttct tcacctaaag ctctttgact tgagaaacca atagagtccg ttttccccgc
      601 atctggcaaa gaatgtgcaa tgtagatatc acggccatca aagttaggta tgtaattatc
      661 aatatttcga atacgctttt tctgaataag catggatcgt tccttttgat tttatcttac
      721 tgtttacata accaaccaag ccgggcacaa tcgagccgtt agaaacgata gtgaggctaa
      781 caatcgttat gagtttgcag actcatggcg gtcatgcttc atagaccgga ttcacgcagg
      841 tgactccggt ttttttatgc ccatcattta tacatgagca caacatatag ggtgtgttaa
      901 taagattacc ccctacatgt tcagcattct agttaggatt ctttgcagta caagggttga
      961 ttttgaggtt aaattgaggt tgtcaaaggg aaatttcgac caaccctacg tgccgtaagg
     1021 cttaggctgt atgtcaatgt ttcagaaaaa aaatgaaaat ttgatcgagt gctgatttct
     1081 ttagcacgag aacatgagat cgaaccaaaa tattgcaaag ttttgaacaa aacttgagct
     1141 gcctcgtgtc tccatagcca agacgaatgc agtttaaata agctaagtca gctctcaaaa
     1201 gtcctttatg acccgaacca aaaattgtac ctcttaaacc cccaccgcaa gcggctctcc
     1261 ggggtttgtg cgcgtggagt aatgcgattg tactgggtat gtgcgcacaa ccctctacaa
     1321 gccgcattct accgttagcc tatagctgaa tccccggctt gaaaaattgc acgcttatgg
     1381 ttgcccctcc ctcaactgta tagctcgccc gcgcccccgt gcgcatgtgc gtgcccgcgc
     1441 gtcaggggtc gctgagaaaa ccctaagatt ttgctcatcc gagacaaccg ttgcaggcgc
     1501 gcgttgccgg ggcacactgt tagtgtttga tggtgtcatc aatcgaccgt tttctggcac
     1561 ctcccaatcg cacgctttca cactgttgga aaaatagcct tggcttttct ggcaggccat
     1621 cctaatgttg acctcgtttt ctggcagggg tcagatgggg agataacaat ggcaactttt
     1681 ggctacggac gtgtttcgac atcgcagcag gatactgaga accaacggct ggagcttggg
     1741 caggctgggt ggcaaattga ttactggttt gccgatgttg ttagcgggaa ggttccggca
     1801 atgcaacgta aagcattttc tgaaatgctc aataaaatcc gcgatggtga aacgttggtg
     1861 gtagcgaagc ttgatcgtct ggggcgtgat gcgattgacg tcctgcaaac ggtaagggcg
     1921 ctggctgacc gtaatatcaa ggttcttgtc caccagcttg gtaacacaga tctcacttct
     1981 gccgctggga agttgctatt atctatgctc gccgcggttg cggagatgga gcgtgatctg
     2041 ctgattgagc gcactcaggc gggactatca cgagcaaaag ccgaaggtaa gaaattgggc
     2101 agacctccta aaatcgcgtc agaagcccgt agagagattt tagagaaaaa gaatagcggt
     2161 ttgagtatca gtgcgttagc gcgtcaatat ggcgtttcaa gagcgactgt agcagctatt
     2221 accgattgaa tatggagagt ttatggctta cacagcaact gttatccctg taatgattgc
     2281 ttctcctggt gatgtcgctg aagagagaca agtcattcgt gaaatgatcc atgagtggaa
     2341 tgatataaat tcagctcgtt caaaagtaat gttgacacca attggatggg aaactcatac
     2401 atcacccgaa ttaggagtta ggcctcagaa gttgattaat caaagattat tagtagattg
     2461 tgatttatta atcggtgtgt tttggactcg tctaggctca ccgactggga acgaggccag
     2521 cggaacagtc gaagaaatcc accgtcattt gaatgcggga aaaccggcaa tgatttactt
     2581 ttcctcaaag cctgttgccc ctgaaagcct tgatagagaa caatatgagt ctcttaaatt
     2641 gttcaaaact gagtgcatgc aaaagggatt gatagagtct tttaatgact tatctgattt
     2701 caaagacaaa gtccgtaggc aactttcgat aattatttct agtagccctt atttgtcttc
     2761 tcttatatct accattaaca attctcctga tgcgaataca agtcaatctt tgccagaaag
     2821 taatttgtcc gccgatgcac tcagtttgct gaaattggca tgtgtcgatg atagcggaac
     2881 aatttatgtg ataaggcatt taggtggcac ggatattcaa gcgggaaatc aatcctttgg
     2941 tggaagttcg gctagagagg tagcccgttg ggagggcgca cttaacgaac tgttatcctt
     3001 tgattatgtg attgaaagag gggcaaaagg acaaatgtat tatgttactc ataaaggctg
     3061 gacatttctt gaatctttaa atgaataatt aaatgcgagg ccattatata agtttatttt
     3121 tgatgtgagg gtgaattccc tcacattttt attcgaaaga ggccgcaatg acttctcctg
     3181 tttttgcgtt gaaaacacaa aagtattgaa gcggagctag catccccaag ccgttaacta
     3241 ccttagcttg ttgcccaaat gcctgaataa tcttcgcatt ctcatcgtac cagagataag
     3301 tattaaatac aggcgcttcg ctttcttgcc acacatggcg aaacttcgct ttctcatcca
     3361 ttaccttttt gcactccctg accgggttat ggtgcgtttt cgcatagcat tccgggttgt
     3421 catctgcaca tacgatatct tttcgtgctg tctgatgctc agcacctaca gcagaaaagg
     3481 cgcatacgcc cagcaccaat gagagagtgc ccagagcacc ccatacagtt gcgcgtagcg
     3541 tgctgatgtg ctgctttgat acagcatggg caactacact ataactggcc cagctaatcg
     3601 cggctcccgc tgcaatcggt agtaaaaagt ctgttcccgt catgattccc tcactttcca
     3661 aaccacatcg cgcctgatag cgaacgctgg tgacgcccaa ccgatgatga tcatcatact
     3721 tgttgggttt taaatgtcac cattggtgtt tgttttgaat caaaaaggat tgtcatgata
     3781 cagccgatga atgctagtga ctaagctcgc tgacgtgaga ttggattcaa aatatctcat
     3841 atggagcgtg gccttacagt ggtcaaaatt gcacattcag tgcatgaaaa aatccctgag
     3901 aatacagttt ggcgagcggg taaaagaact gcgcattgcc accggattga gccaggaggc
     3961 ctttgccgat cggtgcgggt ttgcacgtag ttatatgagc agaattgaac gtggtggctc
     4021 taatgcgtct ctggatgcga ttgaggtgct ggctaatgct ctgagcgttg agccgtggca
     4081 gttgttagcg cctggcccat ctgaagataa cgactcggaa ctgttggttc catacgcagc
     4141 cgatggttcc tgttttcacc ccggtttggc gagcacccgt gatggctcgt ttggcgtagg
     4201 tgataaagcg gctcagaagc gttttggcac gtttgctgaa gctcttgagt atcttcgtag
     4261 catggagaca gcaaaatggc gtaggcctaa tcctagcggc aactggggta tcgtatcagc
     4321 ggttcggtgg gacaaactga gaaagtagct caagtgtaag tttatgaatt ttcatgctgt
     4381 atggatgaac agtattcatt agatgctgat agtcttatgt ttcatcagcc ctttgtttct
     4441 ataagagtga ataaaacagc aaaatgcggg tgcgttacgt aggcgttacg gtggcgtttc
     4501 gctttggctc ttaatggctt aattcatata aataacattg agttaaatag ttattctgaa
     4561 caaggtctgc cggggtctaa ctggtttcaa aatccgaacg cagtggcgtt tattcaacgt
     4621 ccacatagct gacaaatgtt ctttcctccc gactcggcag acctccagaa acattaaaag
     4681 gcaggtatgg cacgttgatg ttttatgccg ccacgaacac tcggtttgat tccgtccagc
     4741 ggatcaaact taatatgtag cccctagaac aaaaaggtcc tacgattgct ctcttaacag
     4801 ctaacaggaa cgtgattaca gcaggttata gagacttagg ttcgagtccc ggccttcgca
     4861 ccacatcgta tgcaaatcga gtcgctcaag ggcggctttt tttgcgtctg ggatccatta
     4921 gttacctatc tttctcgcta ttttaactct ctcagagcca accaatttca acccatcgca
     4981 aacgaaaaat gttggtacag gcgttggtat tttcggttcg atattaattt gtattagctc
     5041 agtcctgatc tgacagctat ctgccagatc aggttcggat taatacatta tatctgcccg
     5101 cctacacctt taaacttctc aataaacgcg acgatcttct ggaaaaccgt ctgttttttc
     5161 gtcttatatt gcggatttaa cggactgagt tttggcaatg tctcgtttaa ttccgtgcca
     5221 ttttcggttg cgtattcgcg ttttaaagac gtgcgaatat agcgtttcgc cgcctcttca
     5281 ttgagatttt cttctttgat caatgctgct gcttcacgtt gctgttcgcg ttgagcaaag
     5341 gtaaaaaacg cgtcaatgat gctggcttta tccggcaaat catccaggtt cgtttgctga
     5401 ataaaatcga ccaccaatcc ttctttagca cggttcccaa ggcttgaacg aattaagcgt
     5461 ttgacctctt cggtcatttc gcccttacct ttgttttgtc tgttgtgttc gaaaatcagt
     5521 ccaaggatat aatccaggtt tatttcctga gacttcagca aatcgatttc aaaaactacg
     5581 tcatcccagt cagtggttga tttttctttt ttgtcagctt ctttctcacg gcgctgccag
     5641 tcacgaatat cgttataggc agaacgataa tcctgaatct tgcgttcagc agggagacga
     5701 atc
//
LOCUS       DAOFQD010000208         5680 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.209, whole genome shotgun sequence.
ACCESSION   DAOFQD010000208 DAOFQD010000000
VERSION     DAOFQD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.209"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..453
                     /gene="pdeC"
                     /locus_tag="QBF46_004565"
     CDS             <1..453
                     /gene="pdeC"
                     /locus_tag="QBF46_004565"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDQ6676662.1"
                     /translation="KTEQYAVRPQQIKFEVTEHAFLDVEKMTPIILAFRQAGYEVAID
                     DFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHIIELAHSLGLKTIAEG
                     VETEEQVNWLSKRGVRYCQGWFFAKAMPPQVFMQWMEQLPARELTRGQ"
     gene            complement(456..779)
                     /gene="soxS"
                     /locus_tag="QBF46_004566"
     CDS             complement(456..779)
                     /gene="soxS"
                     /locus_tag="QBF46_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDQ6676663.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            865..1329
                     /gene="soxR"
                     /locus_tag="QBF46_004567"
     CDS             865..1329
                     /gene="soxR"
                     /locus_tag="QBF46_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDQ6676664.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            1875..3224
                     /gene="ghxP"
                     /locus_tag="QBF46_004568"
                     /gene_synonym="yjcD"
     CDS             1875..3224
                     /gene="ghxP"
                     /locus_tag="QBF46_004568"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDQ6676665.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3376..5025
                     /locus_tag="QBF46_004569"
     CDS             3376..5025
                     /locus_tag="QBF46_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDQ6676666.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5179..>5680)
                     /locus_tag="QBF46_004570"
     CDS             complement(5179..>5680)
                     /locus_tag="QBF46_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDQ6676667.1"
                     /translation="PYVNLFKADLSFSKLDLINFEHADLSRVNLNKSILQNINLIDSK
                     LFFTRLTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSVLTKAWMFNTRLYRVNFDE
                     ASVQGMGISILRGEENIPINSDTLVTLQKFFEEDCATHTGMSQTEDNLHAVAMNITAD
                     IMQHAD"
BASE COUNT         1247 a         1332 c         1562 g         1539 t
ORIGIN      
        1 aaaacagagc aatacgcggt gcgtccgcag caaattaaat ttgaagtgac tgagcatgca
       61 tttcttgatg tcgaaaaaat gacgccgatt attctggctt tccgccaggc tggttacgaa
      121 gtggcaattg atgattttgg tattggctac tctaacttgc ataaccttaa atcattgaat
      181 gtcgatattt tgaaaatcga taaatcgttt gttgaaacgc tgaccaccca caaaaccagt
      241 catttgattg cggaacacat catcgagctg gcgcacagcc tggggttaaa aacgatcgct
      301 gaaggcgtcg aaactgagga gcaggttaac tggctgagca aacgcggcgt gcgctattgc
      361 cagggatggt tttttgcgaa ggcgatgccg ccgcaggtgt ttatgcaatg gatggagcaa
      421 ttacccgcgc gggagttaac gcgcgggcaa taaaactaca ggcggtggcg ataatcgctg
      481 ggagtgcgat caaactgccg acggaaaacg cgggagaagg tctgctgcga gacataaccc
      541 aggtccattg cgatatcaaa aatcggacgc tcggtggtgc gcaactcaac ggcggccagt
      601 aacaggcgac gttggcgaat gtaatcgcca agcgtctgat gcgtcaccgt gcggaacatt
      661 cgttgcaagt accattttga atagcctgat ttttttgcga ctacatcaat gttaagcggc
      721 tggtcaatat gctcgtcaat ccatgcgata agatcctgaa taattttctg atgggacata
      781 aatctgcctc ttttcagtgt tcagttcgtt aattcatctg ttggggagta taattcctca
      841 agttaacttg aggtaaagcg atttatggaa aagaaattac cccgcattaa agcgctgcta
      901 acccccggcg aagtggcgaa acgcagcggt gtggcggtat cggcgctgca tttctatgaa
      961 agtaaagggt tgattaccag tatccgtaac agcggcaatc agcggcgata taaacgtgat
     1021 gtgttgcgat atgttgcaat tatcaaaatt gctcagcgta ttggcattcc gctggcgacc
     1081 attggtgaag cgtttggcgt gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa
     1141 cagctttcgt cccaatggcg agaagagttg gatcggcgca ttcatacctt agtggcgctg
     1201 cgtgacgaac tggacggatg tattggttgt ggctgccttt cgcgcagtga ttgcccgttg
     1261 cgtaacccgg gcgaccgctt aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa
     1321 caaaactaaa gcgccacaag ggcgctttag tttgttttcc ggtctttgtc tttctctcta
     1381 tcccgctggt acacaggagg gtttcccccg acgtcaacac acctcattcg agcacgtggt
     1441 ggaggttccg gttggtgttg atgctttaat tgtatgtcac cgacgtttct tcgccagtgt
     1501 aaaagtatac tttttaaccg caatattttt gtcatctcag acgatttttt atcgcaatcc
     1561 tgaacggtat acggctcgat aacgctgcaa tcttgcgcac cgacgataac gtttgcgcat
     1621 caattgcctg gtttttcatc gtcaagacaa taaaagagaa aaaagcagca aacttcggtt
     1681 gaaaaagccg ctatgatcgc cggataatcg tttgcttttt ttaccacccg ttttgtatgc
     1741 gcggagctaa acgtttgctt ttttgcgacg cagcaaattg tcgcaaacct ggagcaggaa
     1801 gataacgttt cgctggcagg ggattgtccg ccacgcatct tgacgaaaat taaactctca
     1861 ggggatgttt tcttatgtct acgccatcag cgcgtaccgg cggttcactc gacgcctggt
     1921 ttaaaatttc acaacgtgga agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt
     1981 ttctggcgat ggtctactcg gtcatcgtcg ttccaggtat gttgggtaaa gcaggcttcc
     2041 cgcctgcggc agttttcgtt gcaacctgtc tggttgccgg actcggttct atcgtgatgg
     2101 gtctgtgggc taatctgccg ttggcgattg gttgcgccat ctccctgacg gcatttaccg
     2161 cattcagcct ggtgctgggg caacatatta gcgtacctgt cgcgctgggt gccgtgttcc
     2221 tgatgggtgt gctgtttacg gtaatttctg ccacgggtat ccgtagctgg attttgcgca
     2281 acttgccgca cggtgtggcg cacggcacgg ggattggtat cggtctgttc ctgctgctca
     2341 ttgccgctaa cggtgtcggt ctggtgatta aaaacccgct tgatggtctg cccgttgcgc
     2401 tgggtgattt cgcgaccttc ccggtgatta tgtcactggt aggtctggcg gtgatcatcg
     2461 gcctggaaaa actgaaagtc cctggtggca ttctgctgac cattatcggt atctcaattg
     2521 tcggtttgat cttcgatcct aacgtccatt tctccggcgt tttcgccatg ccttcattga
     2581 gcgatgaaaa cggcaactca ctgattggca gcctggacat tatgggcgcg ctgaatcctg
     2641 tagtcctgcc aagcgttctg gcgctggtga tgacggcagt atttgatgcc accggaacta
     2701 tccgcgctgt cgccggccag gcgaacctgc tggataaaga tgggcagatc atcgacggcg
     2761 ggaaagcact gaccactgac tccatgagca gcgttttctc tggcctggtg ggtgcagctc
     2821 cggcagcggt atacatcgag tctgcggcgg gtacggcggc gggcggtaaa accggtttga
     2881 cggctatcac cgttggcgtg ctgttcctgc tgattctgtt cctctctcca ctctcttacc
     2941 tcgttccggg gtacgcaacg gctccggcgc tgatgtacgt tggcctgctg atgctgagca
     3001 acgtggcgaa aatcgacttt gctgattttg ttgatgcgat ggcgggtctg gttacggcgg
     3061 tattcatcgt gctgacctgt aacatcgtaa caggcatcat gatcggcttt gcgactctgg
     3121 tgattgggcg tctggtttcc ggcgaatggc gcaagttgaa catcggtacg gtcgttatcg
     3181 ccgtggcgct ggtgaccttc tatgcgggtg gctgggctat ctaatctttc atcggattct
     3241 gaaaacgggt ggcaatggct gcccgttttt attttctccg cacattgtgt gagcttttgc
     3301 gatattctga aaaaatgaga attcaggcat aacgcctttc tcagatcata aaaaaacatc
     3361 gcaaacaggg aacgcatgga aatcttcttc accatactga taatgaccct cgtggtctcg
     3421 ctgtccgggg tggtcactcg tgtcatgccc tttcagatcc cgcttccgct tatgcaaatc
     3481 gccatcggtg cgctactggc gtggccgacg tttggtttgc atgtggagtt tgatcctgaa
     3541 ctctttttag tcttgtttat cccgccgttg ctgttcgctg atggctggaa aacgccgacc
     3601 cgtgaatttc tcgaacatgg tcgagagatt ttcggcctcg cgctggcgct ggtggtggtc
     3661 accgtggtcg gcattggctt ccttatttac tgggtggtgc cgggcattcc gctgatcccc
     3721 gcctttgcgc tggcggcggt actttctccg accgatgctg tggcgctctc cgggattgtt
     3781 ggcgaagggc gcatcccgaa aaaaatcatg ggcattttgc agggcgaagc gttgatgaat
     3841 gacgcctccg gcctggtgtc gttgaagttt gccgtagcag tggcgatggg gacgatgatc
     3901 ttcaccgttg gcggtgcgac ggtcgaattt atgaaagtag ccattggcgg tattctcgcc
     3961 ggttttgtgg tgagctggct gtacggtcgc tcgctgcgat tcctcagccg ctggggcggt
     4021 gatgaacccg cgacgcagat cgtcctgttg ttcctgctgc cattcgcttc ttatctgatt
     4081 gccgaacata ttggcgtttc cggcatcctc gctgcggttg ccgccgggat gaccatcacc
     4141 cgctccggtg ttatgcgccg tgcgccgctg gcaatgcgcc tgcgtgcaaa cagcacctgg
     4201 gcgatgctgg aatttgtctt taacggcatg gtgttcctgc tgttaggtct gcagctaccg
     4261 ggtattctgg agacgtcgct gatggcggca gaaatcgacc ctaacgtcga aatctggatg
     4321 ctgtttaccg atattattct gatatatgcg gcgctgatgc tggtccgttt cggctggctg
     4381 tggacgatga aaaagttcag caaccgcttc ctgaagaaga agccgatgga gtttggttcg
     4441 tggaccacac gagaaatcct gatcgcgtct ttcgccgggg tgcgtggggc gatcactctg
     4501 gccggtgtgc tctcaatccc gctgctcttg ccggatggta acgtcttccc ggcgcgctat
     4561 gagctggtgt tcctggcggc tggtgtcatt ctcttctcgc tgtttgtcgg cgtggtgatg
     4621 ttgcctattc tgctacaaca cattgaagtc gcggaccatt cgcaacaatt gaaagaggaa
     4681 cgtattgcgc gagcggcaac ggcagaagtg gcgattgtgg cgatccagaa aatggaggag
     4741 cgtctggcgg cggataccga agagaatatc gataaccagc tgcttacgga ggtcagttct
     4801 cgcgtcattg gtaacctgcg tcgtcgcgcc gatggacgta acgacgttga aagttccgtg
     4861 caggaagaga accttgagcg tcgcttccgt ctggcggcat tgcgttctga acgtgctgaa
     4921 ctgtaccacc tgcgcgccac gcgggagatc agcaacgaaa cgctgcaaaa attactgcac
     4981 gatctcgatt tgcttgaagc gttgttgatt gaagaaaatc agtaagccga aagcgacgta
     5041 accaggcccg aggtaatctg aaaaaggtgc aatcttttgc acctttaaca ggttagctcg
     5101 ggctaacatt catgattcta aaacaaataa gttatctctc ttttaaagtc atttcattgg
     5161 ctgttcttcg acttaatctc aatctgcgtg ttgcataata tctgcagtaa tattcatagc
     5221 gactgcatga agattatcct cagtttgtga catgccagta tgagtggcac aatcttcttc
     5281 aaagaatttc tgtagtgtta ccagggtatc actgttaatg ggaatattct cctccccacg
     5341 gagaatggaa atgcccattc cctggacgct agcctcatcg aaattaaccc gatagagacg
     5401 ggtattaaac atccaggctt ttgttaaaac agaacagttg aaatggcaat tgcttaaatt
     5461 ggcattatta aaattaacat tagccatatt agaatcggtg catataacca tttcgaggaa
     5521 cgtatttgtc agccgtgtaa aaaagagttt actatcaatt aagtttatat tctggaggat
     5581 tgatttgttc aggttgacgc gagacagatc agcatgttca aagttaatta aatcgagttt
     5641 agagaaacta aggtcggctt taaataaatt aacatatggc
//
LOCUS       DAOFQD010000209         5671 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.210, whole genome shotgun sequence.
ACCESSION   DAOFQD010000209 DAOFQD010000000
VERSION     DAOFQD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.210"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(291..1250)
                     /gene="nrdF"
                     /locus_tag="QBF46_004571"
     CDS             complement(291..1250)
                     /gene="nrdF"
                     /locus_tag="QBF46_004571"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDQ6676668.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1260..3404)
                     /gene="nrdE"
                     /locus_tag="QBF46_004572"
     CDS             complement(1260..3404)
                     /gene="nrdE"
                     /locus_tag="QBF46_004572"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDQ6676669.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3377..3787)
                     /gene="nrdI"
                     /locus_tag="QBF46_004573"
     CDS             complement(3377..3787)
                     /gene="nrdI"
                     /locus_tag="QBF46_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDQ6676670.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3784..4029)
                     /gene="nrdH"
                     /locus_tag="QBF46_004574"
     CDS             complement(3784..4029)
                     /gene="nrdH"
                     /locus_tag="QBF46_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDQ6676671.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4277..4606)
                     /locus_tag="QBF46_004575"
     CDS             complement(4277..4606)
                     /locus_tag="QBF46_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDQ6676672.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4758..5102
                     /locus_tag="QBF46_004576"
     CDS             4758..5102
                     /locus_tag="QBF46_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDQ6676673.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5139..5588)
                     /gene="alaE"
                     /locus_tag="QBF46_004577"
     CDS             complement(5139..5588)
                     /gene="alaE"
                     /locus_tag="QBF46_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDQ6676674.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1452 a         1403 c         1413 g         1403 t
ORIGIN      
        1 tctaccctaa catactgaga atctgaggca acccctgagg gcaaatgtgg catgaatatt
       61 gatgcgagca aatcgccgga atttcccgtg atataagggc tgagagcaaa tcgagaaaag
      121 ttgatatttt ttgtcataga tagcgaaaaa aagcctgaat attgtagttt caggcgaata
      181 atgcaacaga gtatcagtgt agatcactac agaatatttg cgtgataaaa agctatttgg
      241 cgggaattat ttccctgctg cgggtagtga tatttttgaa aataacaccc tcagaaattc
      301 cagtcttcat cttctgtttc aaccgctttc cccatcacat aagaggagcc tgaaccggaa
      361 aagaaatcgt gattttcatc ggcattcggc gaaagcgcgg cgaggattgc cggattcact
      421 tccgccattt ctgcgggaaa taacggttcg tagcccagat tcatcaaagc cttattggcg
      481 ttgtaacaga gaaacgcttt cacatcgtca gcccacgggg tttcggcgta cagctcatcg
      541 gtgtattgca actcgttgtc gtagagttcc agcaacaaat cgaaggcgaa actcttcaac
      601 tcttcacgtt gtcccagaga tatcttttcc atgtttttct gatatttata gcctatgtag
      661 taaccgtgga ctgcttcatc gcggataatc agacggatca gatctgcggt attggtcagc
      721 tttccgcggc tggaaaaata catcggcagc cagaaaccgg aatagaacaa aaaagattca
      781 agaaacacac tggcgatttt ctttttcagc ggatcatcac cgcgataatg ttgctgaata
      841 atctgagctt ttcgctgcaa cggtgcgttt tcttcactcc aggcgtaggc ggcatcgaca
      901 tctttggtct gacacagcgt cgagaaaatc gaactgtaag agcgggcatg aaccgcttcc
      961 ataaagctga tattcgataa taccgcttct tcatgaggcg tgagtgcatc gggcatcagc
     1021 gaaggcgcgc cgataacatt ttgcagcgtg tcgagcagcg tcaggccagt aaaaacgcgc
     1081 atcgtcagtt gttgttctac gacagttaat gtctgccagg caggaatatc gttcgacagc
     1141 ggcacctttt ctggtagcca gaaattgctg gtcagacgat tccacacctc cagatcttta
     1201 tcgtcagata tcttgttcca gttgatggcg ctgatacgtg agagtttcat agatattcct
     1261 taaagtgcac aggagacgca gccttcaatt tcagtgcctt ccagcgccat ctgacgcagg
     1321 cggatgtaat agagcgtttt gatacccttg cgccaggcgt aaatctgcgc tttgttgata
     1381 tcgcgagtgg tggcggtatc ggggaaaaaa agcgtcagcg acagcccctg atcgacatgg
     1441 cgagtcgctt ccgcgtaggt gtcgatgatc ttttctgcgc caatttcgta agcgtcctga
     1501 tacagcgcca gattctcgtt agtcataaac ggggcagggt agtaaacgcg tcctgttttg
     1561 ccctctttgc gtatctctac tttcgccaca atcggatgaa tactcgacgt agcatggttg
     1621 atataagaga tagaaccggt tggcggcacc gcctgaagat tctggttgta tatgccgtag
     1681 cgcatcacgt cgtcgcgcag ctgcgcccac atctcacggg taggtaacgt aataccgcta
     1741 cgggtaaaca gttcgccaac tttcgccgtt ttcggctgcc agttcccttg cagatattgg
     1801 ctaaaatatt caccactggc atagcgtgac tgtttgaacc cggcgaaggt ttcaccgcgt
     1861 tcgcgtgcca gcaacatcga ggtacgcagt gcatgccagg tgatggcata gaaatagaga
     1921 ttggtgaaat ccagtgcttc cggcgaacca taagcgatgc cttctcgcgc cagatagccg
     1981 tgtaaattca tctgccccag tccgatggcg tgcgaggcgg catttccggc ttcgatggac
     2041 ggcacgctgc ggatatgact catatctgac accgctgtta aaccacgtac ggcagtctct
     2101 accgtgcggg caaagtcggg ggaatccatg gtgtgagcaa tattcaacga acctaaattg
     2161 caggagatat catggcctgt gcgggcatag tcgagattct cgtcatactc tgaggcacag
     2221 ttgacctgca aaatctctga gcaaagattg ctcatattta tgcgcccggc gatagggtta
     2281 gcacggttta ctgtgtcttc atacatgatg tagggatagc cggactcaaa ctggatttct
     2341 gccagtcgct ggaagaaatc acgggcgttg aggtattttt tgcggatgcg ttcatcggca
     2401 accagttcgt catagtgttc gctaatggcg atatcggcaa acggcttgcc ataaactcgc
     2461 tcaacgtcat agggcgaaaa cagcgccatc tgcgcattct cttttgccag atggaaagtg
     2521 atatccggga tcaccacgcc cagcgacagt gttttaatgc ggattttttc gtcggcattt
     2581 tcccgttttg tatcgagaaa acgcagaata tcgggatgat gagcatgtaa atagactgca
     2641 cctgccccct gacgcgcgcc gagttggttg gcataggaaa atgcgtcttc cagcattttc
     2701 atcaccggaa ttacgccaga agattgattt tcaatacgtt taattggcgc gcccgcttcc
     2761 cgcagattcg acagcaaaaa tgctacgccg ccgccgcgtt tcgacagttg cagtgcggaa
     2821 tttaccgccc gaccaatcga ctccatattg tcttcaatac gcagcaaaaa acaggaaacc
     2881 agttcgccgc gctgctgctt accgcagttg aggaatgttg gcgtggctgg ctgaaagcgt
     2941 cctgacagca tttcatcggt cagttgcaac gccagcgtct catcgccttg tgccagcgtc
     3001 agcgccacca tcgtgactcg atcggcaaaa tcttccagat aacgtttacc gtcgaatgtc
     3061 ttcaacgtat agctggtgta aaacttccat gccccgagga atgtctggaa acgaaaaccg
     3121 ctggtgtgtg cgtgggcaaa cagcgtaatg acaaagtcgc gagagtagcg attaagaacg
     3181 ctttcatcat agtaaccttc gttgaccagc cagttcaggc gctgctgctg gctactgaag
     3241 gtcacactgt tcggacgcac atgcgtcgca ataaaggcgt caacggcctg gcggtcttta
     3301 tcgaactgaa tgcgacctgc gctatcgtag aggttaagca tcgcattcag cgcgtggtaa
     3361 tccatcgttt cctgcgtcag gcattctgcg gttgtcgttg ccaaaattcg gttactcctt
     3421 tacgaacgtt ttcgatatcg ctttgcgtac ccatgagttc aaaacggtac agccacggca
     3481 cgccgcattt ccgggcaatc acatctccgg cgcggccata cgcctcacca aagttgcgat
     3541 taccagaagc aataacgccg cgaagcaacg cccggttgtg ctcgtcgttt aaaaagcgaa
     3601 ttacctgtcg tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc acgatcagga
     3661 tgtaaggctc gtctacctga atccgttccc gctcattgag cgggatgcgc accgcgggca
     3721 gacctaaacg ttcgataaaa cgctgcgtgt tttcggagct gctggagaag tagacgagct
     3781 ggctcatgca ctggccgcgt gtggcgctgg atgcagacgg ttaatcatgt ccggacggaa
     3841 accagaccag ctaagatcgc cagcaatcac taccggcaac tgacgaaagc cctgagcacg
     3901 caacgcttct gccgcttcag gaacgcgatc gacattaatc atttcaaaat caaagccccg
     3961 attttccatc gcccgtttgg tggcgtggca ctgaacgcaa tcgttacgag tataaatagt
     4021 aatgcgcatg attcgtattt ccgtttgaaa tgaagatacg gcgcgatgat acgcgtcggg
     4081 ttgtctctct gttgatacag agatactaga tgtagttgaa aaaagattca accacacaat
     4141 atatagcaaa tagtggtcga aattaccctg gatatgagcg tgacggggta gggggatttt
     4201 tgtgattcac caggcaaaaa gaaaccccga agacaggctt cggggtcaaa gacgcgtatt
     4261 tattatcatt tttgcactac gatttgcgca tgcttaacag tgcgccgata aaaataccta
     4321 ccgcagctgc tgtacccacg ctacaccagg gtctttcacg aacaaaagaa tccgcgcagc
     4381 caacggcatc gcgtgcggct tgctggacgc gagtacgacc atgcattcgc gccctggttt
     4441 ctttcagcaa tgcctgagct ttactgcgtg cggcttcagc ttcccctttg gcgtcgctgc
     4501 cccaggactt caatacagat tcgaggctgt cagctaattg attgacatca ttctggatat
     4561 cctgcacgcc atcatcaacg tcgttgcggt tcggtcggtt aaacatatga tcccccatta
     4621 gaattcgatg taattttcag tgtagaccat aattttctta actgaggttc atcgcggctt
     4681 tatggacttt tctgaaagcg ttgtgaatgc gcaatcaggt aaggtaggcc cgcggttgat
     4741 aataacgagg atcaaatatg tatttacgac cagacgaggt ggcgcgcgta cttgaaaaag
     4801 tcggttttac tgtcgatgtg gtaacccaga aagcgtatgg ttaccgccgt ggggaaaatt
     4861 atgtctatgt taatcgcgaa gcgcgaatgg gacgtaccgc actggtaatt catccaacat
     4921 taaaagaacg tagttcgaca ctggctgaac ccgcttccga tattaaaact tgcgatcatt
     4981 atcaacaatt tccgctctat ttagcaggcg agcgacacga gcattacggt atcccgcatg
     5041 gctttagttc gcgtgttgcg cttgaacgtt atttgaatgg tttatttggc gaagccagtt
     5101 aaagacgcga ctggcgatgc cagtcgcgaa aagaagggtc aggcttttac ctgctggtaa
     5161 cggctgactt taaacagtcg gcggcaataa tcgaggaagt agccataaac cgcccccatc
     5221 aacatcgaaa caacgatgtt tgaactgacc gccgccataa tctgatgcca gtctgcgccc
     5281 accactaaca agatcgccac atacaccggt gactggaacg tcacataagc cagaatatcc
     5341 gccagatttt ttatccagcc cgacgggcta actttgcgtg ccgcgcgcat aaacagatca
     5401 cggtacatac cgtatggcca tgcaattaag atgttcaccg gaatcgctac caatctggaa
     5461 taaaaagact gttcgaagct cattccggag aggaaaactt caatacacat gttcacgaca
     5521 gaacagtaaa caaccatcgc gaacgtatct gcaactgcat gacgcaagcg tgactgcggt
     5581 gagaacatgc taatgctcct taataaaagc gtaatgggat gttaatggag atgaaagcag
     5641 tggatctcat tgccatctgc attttcgcat a
//
LOCUS       DAOFQD010000210         5523 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.211, whole genome shotgun sequence.
ACCESSION   DAOFQD010000210 DAOFQD010000000
VERSION     DAOFQD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.211"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(212..772)
                     /locus_tag="QBF46_004578"
     CDS             complement(212..772)
                     /locus_tag="QBF46_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDQ6676675.1"
                     /translation="MSVQENTINILHELESNALKIDDAQAAQFISQIRNARHIFLQGA
                     GRSGIAIRAFANRLLHLGFSVSVVGEILSPHTQPGDLLIIGSGSGETTSLKSLAQKAV
                     DSGVNVALVTMKADSTIGKLAQSVLVLPGSVKDNNNRVAGAFAQPMGSAFEQLCFITY
                     DAIVLQLMSELGESSATMFTRHADLE"
     gene            complement(788..1540)
                     /locus_tag="QBF46_004579"
     CDS             complement(788..1540)
                     /locus_tag="QBF46_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6676676.1"
                     /translation="MSNFAGKVVLVTGGAHGIGKGIAKQFADRGAHTVIVDYNEENGK
                     ATAAELTFGDVKSLFIKTDVSDPQALVDVREKVFATFGRIDVLILNAGVAFANKVNNI
                     SFEEWNKTIAINLSGLFNTVKAFYDDFLTNKGSIVYISSGSALSGTGGGVSYPASKAG
                     GEGLIRGLAKELGPKGVNVNAIAPRLIDTGEMMRVNYPTQADLDAVLEKIPVRRLGTI
                     DDVANLAIFLADKDNSYIQGQTILMDGGRTIA"
     gene            complement(1638..2282)
                     /gene="rpe"
                     /locus_tag="QBF46_004580"
     CDS             complement(1638..2282)
                     /gene="rpe"
                     /locus_tag="QBF46_004580"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDQ6676677.1"
                     /translation="MAKILPSIFGADILRLKDEIHFLEEEQTEILHVDLMDGTYVSNI
                     AFGPNQIAAMKKASSMIFDVHMMLANPERHIDDVINTGAEMISVHYESTPHVHYIIQK
                     IKKAGRKAGVVLNPGTPESVIEYLLDDIDYILLMTINPGQPGQTFIHKSLEKIRNTKK
                     MISARDIQIEVDGGVNLDNVKQVKEAGADLIVVGGALFNKQPRESYQALKIAVQ"
     gene            complement(2305..3558)
                     /locus_tag="QBF46_004581"
     CDS             complement(2305..3558)
                     /locus_tag="QBF46_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDQ6676678.1"
                     /translation="MEMLKNLMETITGMGATAILPLVIFILGLVFRMKPGAAIKSGIT
                     VGIGFIGLGLVVGLLNSSLQPAIEYYSKVGSGFTVADIGWPAVGAAAWVAPFAALVIP
                     VGIVLNLILVRLKLTKTLNVDIWNYMHFLVPGALAYFVFDSFIIGFSVAVALSIAALF
                     IGDLIAPRWQKYYGLEGTTCTTMIHIGWTLPFAWVVNKIIDYIPGLNKLDVDLNSVQK
                     RPGVFGEPAIIGVIVGALLGVLTKQAITTIVPMAMGVAGVMVLLPKVVGVLMEGLNPI
                     GKSAKEIMQKQMGKDAELNIGMDCALALGDPATVTVTVITIPLTMLCALVLPDIKIFP
                     IGVLMSIIYMTTMTVMASNGNVIRSIISTLLFCVVVMYLGGYVAPGATQFLAGAGVGL
                     QGQGTDFVLTGPWEILTYWLSTVLH"
     gene            complement(3579..3872)
                     /locus_tag="QBF46_004582"
     CDS             complement(3579..3872)
                     /locus_tag="QBF46_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004105500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDQ6676679.1"
                     /translation="MINILVACGSGVATSTLAADEVKSVCAEYGITAFKINKCSMAEL
                     SSEMAHADIVLTTNNYKGDIGKPHMSVAGFVTGINEGALRKKLGELLTELANG"
     gene            complement(3891..4334)
                     /gene="gatA"
                     /locus_tag="QBF46_004583"
     CDS             complement(3891..4334)
                     /gene="gatA"
                     /locus_tag="QBF46_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDQ6676680.1"
                     /translation="MISISVADGAGLQNYDAVLEHIYNTHRATNTVKESWLNAMRKRE
                     AEYPTGIELEGYSIAIPHCDSEHANQPAIYIIRLPDAIPVNRADEDGILWVRLVVNLI
                     VTDPADQLRLLKSLFNHLQNEEFYQNLLTLPVDDAKALFVSKIIQ"
     gene            complement(4662..5417)
                     /locus_tag="QBF46_004584"
     CDS             complement(4662..5417)
                     /locus_tag="QBF46_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6676681.1"
                     /translation="MYLVDERRKLILEDLMSAGTVYVSDLARKYEVTYETIRKDLTHL
                     EQKGLLIKSHGGATLKQRAIEHPFQVREKENNSYKKAIARKALDFIPPNSSMIIGTGS
                     TTFELANLLCMKSGFKIFTDSLPVASVLIESDNQVFLFGGELRRKSSSVFGGWAVSMI
                     NDIHVDLCFIGTDGFSNMTGPSTPSSSDAFLDRTIISHSDKKYILGDYTKFERSSLFK
                     ICDWSEITALITNDHADKEGIAEVAKYTSVITC"
BASE COUNT         1663 a         1286 c         1002 g         1572 t
ORIGIN      
        1 tgattatgac aacaacaaat tgtttttaat tgacattttt accacaagaa aacaataatg
       61 atggtaaata tgttggtaaa aatatggcta tatttaacaa aggcagtaat aacaggagat
      121 acagatgaag ttcgagtcca gtcagaggag ccaaattcaa aaaagcctgc tttctagcag
      181 gctttttgct ttccaagtac caactctttg gtcattccag atcagcgtgt cgggtaaaca
      241 ttgtggcact gctttcaccc agttccgaca tcaactgcag aacaattgca tcataagtaa
      301 taaagcagag ttgctcaaaa gctgagccca ttggttgtgc gaaagcgcct gcaacacgat
      361 tgttattgtc tttcacactt cctggtagta ccaacacaga ttgagctaat ttaccgatcg
      421 tcgaatctgc tttcatagtt accagagcta cgtttactcc gctatcaact gctttttgtg
      481 ccagactttt caaactggta gtttcaccac tgcctgaccc aataattaat agatctccag
      541 gttgggtatg cggggataat atttctccca caacgcttac tgaaaaacct aaatgcagca
      601 agcgattagc aaaagcgcga atagcaatgc cactgcgacc agctccctgc aaaaaaatat
      661 gccgggcatt tctgatttgc gaaataaact gtgcagcctg agcatcgtca atcttcagcg
      721 cattactctc taattcatgc agaatattaa tagtattttc ctgaacactc attttttctc
      781 tcccttttta agcaatagtc cggccaccat ccattaaaat ggtttgtccc tgaatataag
      841 agttatcttt atctgccaaa aatattgcca ggttagccac atcatcgata gtccccagac
      901 gacggacggg tattttttcc aggacagcgt ctaaatcggc ttgtgttgga tagttgaccc
      961 gcatcatctc tccggtgtct attaagcggg gagcaatcgc attgacattg acgccttttg
     1021 gtcctaactc ttttgccagc ccacgaatca atccttcgcc tcccgcttta ctggcaggat
     1081 aactgacacc gcccccagtc cccgaaagcg ctgatcccga tgagatataa acaatagatc
     1141 ccttatttgt caggaaatca tcataaaacg ccttaacggt attaaataat cctgataagt
     1201 taattgcgat agtcttattc cattcctcaa aagaaatatt gttaacttta ttggcaaatg
     1261 ccacaccggc atttaatatc aacacatcaa tgcgaccaaa ggtagcaaat accttttccc
     1321 gaacatccac taatgcctgt ggatcggaga catctgtttt aataaacaac gatttaacat
     1381 cgccaaaagt taactctgct gcagtggcct tgccgttctc ttcgttatag tcaacaataa
     1441 cagtatgtgc accacgatca gcaaattgtt tagctatccc tttgccaata ccgtgagcac
     1501 ctccggtaac taaaaccact ttacctgcaa aattactcat tacttttctc cattaaagac
     1561 attttttagc tgaggttttc ctcccgatat gaaatcgaaa acctcactta actggatgtg
     1621 aaatttttag attgtgatca ctgaacagcg atttttaatg cctgataact ttcacgaggc
     1681 tgtttgttaa ataaggcacc accaacaaca ataaggtcag cccctgcttc tttaacttgt
     1741 tttacattat caagattaac gccaccatcc acttcaattt gaatatctct ggcagatatc
     1801 atttttttgg tattacggat tttctccaga cttttatgaa taaatgtctg gcctggttgc
     1861 cctgggttaa ttgtcattaa caatatataa tcaatatcat ctaataaata ttcgattaca
     1921 gattcaggtg tcccaggatt aagcacaaca cctgctttgc gacccgcctt tttgattttt
     1981 tgaataatat aatgtacatg tggcgtcgat tcataatgta cagaaatcat ctccgccccg
     2041 gtattgatta catcatcaat atgtcgctca ggatttgcca gcatcatgtg tacgtcaaag
     2101 atcatcgaac tggctttttt cattgctgct atttgatttg ggccaaaggc aatattactg
     2161 acataagtgc catccattaa atcaacgtgc aatatttcgg tttgttcctc ttccagaaaa
     2221 tgaatttcat ctttcaggcg taaaatatcc gcgccaaata tagaaggtaa tatttttgcc
     2281 atgttaaaac tccagaatat taaatcaatg taatacggta ctcaaccaat aggttaaaat
     2341 ttcccagggg ccggttaata caaaatcagt accttgtcct tgcaagccta caccggctcc
     2401 agctaaaaat tgcgttgccc ctggtgcgac ataaccgcct aaatacatca ctacaacgca
     2461 gaataacaag gtcgagataa tcgaacgaat cacgttaccg ttgctcgcca ttacggtcat
     2521 ggtggtcata taaataattg acatcaatac gccaattggg aagatcttaa tatcaggtaa
     2581 taccagagca catagcatgg ttaaaggaat ggtaatgact gtcacggtga ccgtcgccgg
     2641 atcccccaac gctagtgcac aatccatacc gatgtttaat tcagcatctt tacccatctg
     2701 tttttgcatg atttctttgg cacttttccc tattgggtta agaccttcca tcagcacacc
     2761 gaccacttta ggtaacaata ccattacgcc agcaaccccc atcgccatgg gaacaattgt
     2821 ggtaattgcc tgtttcgtta aaactccgag taatgcgcca acgataacac cgataattgc
     2881 aggttcacca aagacacccg ggcgtttttg cacactgttt aagtcaacat ctaacttatt
     2941 taaccctggg atgtaatcaa tgattttatt tactacccag gcgaacggta gagtccagcc
     3001 gatatgaatc atcgtggtac aggtcgttcc ctcgagtcca taatattttt gccatctggg
     3061 cgcaatcaaa tcgccaataa acagagccgc aatactcaaa gccaccgcga cagagaagcc
     3121 gatgataaaa ctgtcgaaca caaaatatgc cagagcaccc ggaactaaaa aatgcatata
     3181 gttccagata tcaacattta acgttttggt taatttcaga cgcaccagaa tcaggttaag
     3241 tacgatgcca actggtatca ccaatgcggc gaaaggtgcc acccatgcag cagcaccgac
     3301 tgcaggccaa ccaatatctg ctaccgtaaa gccactacct acttttgagt aatactcaat
     3361 agctggctgt aatgaactat ttaataaacc aacaaccaga cctaaaccaa taaaaccaat
     3421 accgactgtg atgcccgact tgatcgcggc accgggtttc attctgaaca ccagacctaa
     3481 aataaatatc accagcggca gtattgcagt tgcccccatt ccggtgattg tttccattaa
     3541 atttttcaac atttccattt ttattattcc tgtcaggttt agccatttgc taattcagtt
     3601 aacaactccc ctaatttctt gcgtaatgct ccttcattaa tacccgttac aaaacctgca
     3661 acgctcatat gaggttttcc aatatcacct ttatagttat tagttgtgag tacaatatct
     3721 gcatgagcca tctcagaaga aagttcagcc atactgcatt tattgatttt aaatgccgta
     3781 ataccatatt cagcacatac tgacttcact tcatcagcag caagcgtaga tgtagcgaca
     3841 ccgctaccac aagcaacaag aatattaatc ataataaact ccagttttta ctattggatt
     3901 attttggata caaaaagtgc ttttgcatca tcgacgggaa gtgtcagtaa gttttggtaa
     3961 aattcttcat tttggagatg gttaaataat gactttaaca atcgaagctg gtcagcagga
     4021 tccgtaacaa taagattaac gacgagtctg acccacaata ttccatcttc atcagccctg
     4081 ttcacaggta ttgcgtctgg cagacggatg atataaatag ccggctgatt tgcatgttca
     4141 ctatcacagt gtggtatagc gatagaataa ccttccagtt ctattcctgt cggatattcc
     4201 gcttcgcgtt tacgcatagc attgagccag ctctctttca ccgtattcgt tgcacgatgc
     4261 gtgttataaa tatgttcaag cacggcatcg tagttctgta gtcccgcacc atcggcaaca
     4321 ctgatcgata tcatatcctt tccccctcac cccacaatgc tcacatgagc attgaacaat
     4381 cttcatatga gcagttatag cacaacgatt ctgtggataa atgacgagat ctgagaaatt
     4441 aaatccagat cacaaaaaag cacataatgg cgatattatt tgtgctcaca tgagcaaatc
     4501 gtgatttctt cgcaaaaaat gaggtgaaaa tggcaatatg cagatgatgt attcaataga
     4561 ggacgctaag caagcatcgc catggatgct cacttgatga ttgggaaatg attacatttg
     4621 gcaaagctga ttcgaaggga gaaggcgtgt cgtagtgagt tctaacaggt tattactgac
     4681 gtgtattttg cgacttcggc aatcccctct ttgtcggcat gatcgttggt tataagggcg
     4741 gtgatctccg accagtcgca gatcttaaac aaactgctgc gctcaaactt agtgtaatcg
     4801 ccgaggatat attttttgtc tgagtgcgaa attatcgtac gatccaaaaa cgcatcagaa
     4861 gaagaaggcg tcgatggacc agtcatgtta ctaaagccgt cggttccaat aaaacagaga
     4921 tcaacatgta tatcgttgat catagatacc gcccagcccc caaacactga ggaactcttt
     4981 ctacgcagtt cgccgccaaa gagaaaaacc tggttgtcag attcaatcaa cacactggca
     5041 acggggagcg aatctgtgaa gattttaaac ccagatttca tacataacag gttagccagc
     5101 tcaaaagtgg tactgcccgt accgataatc atcgaactat taggtggaat gaaatcaagc
     5161 gctttgcgag caatcgcctt cttataagag ttattttctt tttccctgac ctgaaaggga
     5221 tgctctatgg ccctttgctt taaagtcgcc ccaccatggc ttttgatcag cagccctttt
     5281 tgttcaagat gggtcaaatc tttcctgatg gtctcatacg ttacttcata ctttcgtgca
     5341 agatcactga catagaccgt acctgccgac attaagtctt caagtattaa ctttcgcctt
     5401 tcatcaacca gatacatcct tcctcctgaa atctattgcc catctgagaa aacgccaagc
     5461 atagcacaga tgatgcaaac aaaaacaaac ctaacagtgg aaagtacaaa taaacccaat
     5521 aaa
//
LOCUS       DAOFQD010000211         5299 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.212, whole genome shotgun sequence.
ACCESSION   DAOFQD010000211 DAOFQD010000000
VERSION     DAOFQD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.212"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            5..925
                     /gene="yiaO"
                     /locus_tag="QBF46_004585"
     CDS             5..925
                     /gene="yiaO"
                     /locus_tag="QBF46_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDQ6676682.1"
                     /translation="MAAQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELKLKLFPD
                     STLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTLDGKV
                     GDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLKGLKIRTNNSPMNIAAFKV
                     FGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEVQKFLSLTHHAYSPLLVVI
                     NKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAF
                     SDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            929..2425
                     /gene="lyxK"
                     /locus_tag="QBF46_004586"
     CDS             929..2425
                     /gene="lyxK"
                     /locus_tag="QBF46_004586"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDQ6676683.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSE
                     AQRDLRHPVRTLLPDMTAHQLYQKKYQRYQHLIAALQGFHARIKEHTL"
     gene            2422..3084
                     /gene="ulaD"
                     /locus_tag="QBF46_004587"
     CDS             2422..3084
                     /gene="ulaD"
                     /locus_tag="QBF46_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDQ6676684.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQINAIWGGA
                     RA"
     gene            3077..3937
                     /locus_tag="QBF46_004588"
     CDS             3077..3937
                     /locus_tag="QBF46_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDQ6676685.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            3931..4626
                     /gene="araD"
                     /locus_tag="QBF46_004589"
     CDS             3931..4626
                     /gene="araD"
                     /locus_tag="QBF46_004589"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDQ6676686.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(4658..>5299)
                     /locus_tag="QBF46_004590"
     CDS             complement(4658..>5299)
                     /locus_tag="QBF46_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6676687.1"
                     /translation="RELLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFQKHTGLTPAAWKRRYSKEHINS"
BASE COUNT         1210 a         1460 c         1520 g         1109 t
ORIGIN      
        1 ttctctggcg gcacaatctt tacgtttcgg ttatgaaaca tcacaaaccg actcgcaaca
       61 tattgcggcg aaaaaattca atgatttatt gcaggagaga accaaaggcg agctgaaatt
      121 aaaactgttc ccggacagca ctctcggtaa cgcgcaggcg atgatcagcg gcgtacgtgg
      181 cggcaccatc gatatggaaa tgtccggctc gaataacttt gccgggttat caccagtgat
      241 gaacttgctt gatgtccctt tcctgttccg cgataccgct cacgcgcata aaacgctcga
      301 cggcaaagtc ggtgatgatc tgaaagcctc acttgaaggt aaaggactga aagtactggc
      361 ctactgggaa aacggctggc gcgatgtcac caactcgcgc gcaccggtta aaacccccgc
      421 cgacctgaaa gggctgaaaa tccgcaccaa caatagcccg atgaatatcg ccgcattcaa
      481 agtctttggc gctaacccga tcccgatgcc gtttgccgaa gtctataccg ggctggaaac
      541 ccgcactatc gacgctcagg aacacccgat caacgtcgtc tggtcagcaa aatttttcga
      601 agtgcagaag ttcctttctc tgacgcacca cgcctattcc ccgcttctgg tggtgatcaa
      661 caaagcgaag tttgatggct taagtccgga gttccagcag gcgctagttt catctgcaca
      721 agaagcgggt aactatcagc gcaaactggt tgctgaagat cagcaaaaaa tcatcgacgg
      781 catgaaagaa gcgggcgtgg aagtcatcac cgatctcgac cgcaaagcct ttagcgacgc
      841 actggggaat caggttcgcg acatgtttgt taaagatgtg ccgcagggag ctgatctgct
      901 gaaagccgtg gatgaggtgc aataaaccat gacgcaatac tggctggggt tagattgtgg
      961 cggtagctgg ctgaaagccg ggctgtatga ccgcgaaggc cgggaggcag gcgtgcagcg
     1021 cctgccgctg tgcgcattaa gcccgcagcc aggctgggca gagcgcgata tggcagaact
     1081 gtggcaatgc tgcatggctg tcattcgcgc cctgcttact cattctggtg ttagcgggga
     1141 acaaattgtc ggtatcggca tctccgcaca gggaaagggc ttgtttttgc tggataaaaa
     1201 cgacaaaccg ctcgggaatg ctattttgtc ctcggaccgc cgggcgatgg aaatcgttcg
     1261 tcgctggcag gaagatggca tcccggaaaa actctacccg ctgacccgac aaaccttgtg
     1321 gaccgggcat ccggtgtcgc tgttacgctg gctgaaagag cacgaaccag aacgctacgc
     1381 gcaaattggc tgcgtgatga tgacgcacga ctacctgcgc tggtgtttaa ctggcgtcaa
     1441 aggctgtgaa gagagcaata tttccgagtc caacctctac aacatgagtc ttggggaata
     1501 tgacccgtgc ctcaccgact ggctggggat cgctgaaatc aatcacgccc tgccgcctgt
     1561 tgtcggatct gccgaaatct gcggggagat caccgctcag acagccgccc tgaccggtct
     1621 gaaagcgggt acgcccgttg ttggcggcct gtttgatgtg gtttccaccg cactctgcgc
     1681 cgggatcgaa gacgaattta ccctcaatgc ggtgatgggg acctgggcgg tgaccagcgg
     1741 cataacccgc ggtttacgtg acggtgaagc gcatccgtat gtctatggtc gctacgttaa
     1801 cgatggtgaa tttatcgttc acgaagccag ccctacctct tccggcaacc tcgaatggtt
     1861 taccgcacag tggggagaaa tctcgtttga tgagatcaat caggccgttg ccagcttgcc
     1921 gaaggctggg ggcgatctct ttttcctgcc gttcctgtac ggcagcaacg ccggactcga
     1981 gatgaccagt ggtttctacg ggatgcaggc cattcacacc cgcgcgcacc tgttgcaggc
     2041 catctatgaa ggggtggtgt tcagccatat gacccacctc aaccgaatgc gcgaacgttt
     2101 tactgatgtt cacaccctac gcgtcactgg cggcccggcg cactccgatg tctggatgca
     2161 aatgctggcg gacgtcagcg gtctgcgtat cgagctgccg caggtggaag aaaccggctg
     2221 ctttggtgcg gcccttgccg cccgcgtcgg caccggggtt tatcacaact tcagcgaagc
     2281 ccaacgtgac ttgcgacacc cggtgcgcac cctgctgcca gatatgaccg cccatcagct
     2341 ttaccaaaaa aaatatcaac gttatcagca tctcattgcc gcacttcagg gctttcacgc
     2401 ccgcattaag gagcacacat tatgagccga ccacttctgc aactggccct cgaccactca
     2461 tcacttgaag ccgcgcagcg cgacgtgacg ctgttaaaag acagcgtcga tatcgtcgaa
     2521 gcgggcacca ttctctgttt aaacgaaggg cttggcgcgg tgaaagcctt gcgcgaacag
     2581 tgcccggaca aaatcatcgt tgctgactgg aaggtcgccg acgctggtga aacgctcgcg
     2641 caacaggcgt ttggcgcagg cgctaactgg atgaccatca tctgcgccgc gccgctcgcg
     2701 acggtagaaa aaggccacgc aatggcacaa cgctgcgggg gtgaaattca gatagagctg
     2761 ttcggtaact ggacgctgga cgacgcccgc gactggcatc gtattggcgt gcggcaggcc
     2821 atttatcatc gcggtcgtga tgcacaggcc agcgggcaac agtggggcga agccgatctg
     2881 gcacgcatga aggcgctttc agatatcggc cttgagcttt ccattactgg cgggattacc
     2941 cctgctgacc tgccgctgtt taaagatatc cgcgtgaaag cgtttattgc cgggcgggca
     3001 ctggcaggcg cggcaaatcc ggcgcaagtg gctggcgatt tccatgcgca aatcaacgct
     3061 atctggggag gtgcgcgtgc gtaatcatcc gttagggatt tatgaaaaag cgctggcgaa
     3121 agatctctcc tggccggagc ggctggtgct ggcaaaaagc tgcggttttg attttgtcga
     3181 aatgtcggtg gatgaaaccg acgaacggct ctcacgtctt gactggagcg ccgcacaaag
     3241 gacttcgctg gttgccgcga tgatcgaaac aggtgttggg atcccgtcga tgtgcctgtc
     3301 cgcacatcgt cgctttccct ttggtagccg tgacgaggca gtgcgcgaac gggcgcggga
     3361 aatcatgagt aaagcgattc gcctggcgcg cgatctcggc attcgcacca tccagctggc
     3421 aggttatgac gtctattacg aagaccacga cgaaggcacc cggcaacgtt ttgctgaagg
     3481 gctggcgtgg gcagtcgaac aggcggcggc atcacaagta atgctggcgg tggagattat
     3541 ggataccgcg tttatgaact ccatcagcaa atggaaaaaa tgggacgaga tgctcgcctc
     3601 gccgtggttc accgtctacc cggacgtcgg caacctcagc gcctggggca atgatgttcc
     3661 cgccgaactg aaactgggca ttgaccgtat cgccgcgatc cacctgaaag acacccagcc
     3721 agtcaccggg caaagccccg gacagttccg tgatgtgccg tttggcgaag gctgcgtcga
     3781 tttcgttggc atcttcaaaa cgctgcatga actgaactat cgcggttctt tcctgattga
     3841 gatgtggacc gaaaaagcca aagagccggt gctggagatc attcaggcgc ggcgctggat
     3901 tgaagcgcgt atgcaggagg ctggatttat atgttagagc aactgaaagc cgacgtgctg
     3961 gcggcgaatc tggcgcttcc cgctcaccat ctggtgacgt tcacctgggg caatgtcagc
     4021 gcggtagacg acacgcggca atggatggta atcaaacctt ccggcgtcga gtacgacgtg
     4081 atgaccgccg acgatatggt ggtggttgag atagccagcg gcaaggtggt ggaaggtagc
     4141 aaaaagccct cgtccgatac gccaacgcat ctggcacttt accgtcgcta tgccgaaatt
     4201 ggtggcattg tgcataccca ctcgcgccac gccaccatct ggtcacaggc cgggctggat
     4261 ctccccgcct ggggcaccac ccacgccgat tatttttacg gtgctatccc ctgcacgcga
     4321 cagatgaccg cagaggagat taacggcgaa tatgaatatc agaccggcga agtgatcatt
     4381 gaaaccttcg aagaacgtgg caggagtccg gcacaaatcc cggcggtgct ggtgcattct
     4441 cacggcccgt tcgcatgggg taaaaacgcc gccgatgccg tgcataacgc cgtagtactc
     4501 gaagaatgcg cctatatggg tctattctcg cgccagcttg cgccgcagct ccctgcgatg
     4561 caaaacgaac tgctggataa gcactacctg cgtaagcatg gggccaatgc ctattacggg
     4621 cagtaatccc tcacgccggg gcttcatcgc cccggcacta cgaattgata tgttccttgc
     4681 tgtaacgccg cttccacgct gctggcgtta aaccagtatg tttctgaaaa atctgccgga
     4741 aatagccaac gtcattaaac ccgcacaacg cagccacttc agtgagcgat cgcgcttcgc
     4801 taattaacag cttttcggct tcgcgcactc gctggcgatg gattgcttct gttaacgtca
     4861 gatgaaacac ccgccggtag acgcgcccaa gataatcggc attgcagtac agctcgcggg
     4921 ccagaagcgc cgtagaaagc gggcgatgat attgcgtgcg gatcagtttc tgcgctttcc
     4981 aggcaagggt ggtgcctgcg ccatcggcct gttcatcaag acgcgcgtca cgggtgagct
     5041 gctgcaaaat cagcagcaca ataaactcca gcgccggagt gcgttgatgc ttttcctgtt
     5101 cgcttaagaa ctggcgaaac agggccacca gcgcctgcgg atcggcaacc ttcgtttgtt
     5161 gcggcacgct aagatgagtg agccagcgcg ggtcgctatc cggcgcttta acctcaaaat
     5221 gcagccagta aaatttgaga tcggcgggaa aatcctccac cccaacatgt tgtcgattcg
     5281 gccagagtaa taactctct
//
LOCUS       DAOFQD010000212         5291 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.213, whole genome shotgun sequence.
ACCESSION   DAOFQD010000212 DAOFQD010000000
VERSION     DAOFQD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.213"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            84..845
                     /gene="ydeO"
                     /locus_tag="QBF46_004591"
     CDS             84..845
                     /gene="ydeO"
                     /locus_tag="QBF46_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDQ6676688.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            1247..2929
                     /locus_tag="QBF46_004592"
     CDS             1247..2929
                     /locus_tag="QBF46_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDQ6676689.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2981..4138
                     /locus_tag="QBF46_004593"
     CDS             2981..4138
                     /locus_tag="QBF46_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDQ6676690.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4104..4226)
                     /locus_tag="QBF46_004594"
     CDS             complement(4104..4226)
                     /locus_tag="QBF46_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676691.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
     gene            4429..>5291
                     /locus_tag="QBF46_004595"
     CDS             4429..>5291
                     /locus_tag="QBF46_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDQ6676692.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRS"
BASE COUNT         1621 a         1093 c         1039 g         1538 t
ORIGIN      
        1 caagactgaa aataaacagc aaattataaa tatgaaatgt taaaaaagta tcgataaaaa
       61 ctttattgtt ttaaggagat aaaatgtcgc tcgtttgttc tgttatattt attcatcatg
      121 ccttcaacgc taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg
      181 tagataacgc tgttcgtacg cattttgaac cttatgagcg gcattttaaa gagatcggat
      241 ttactgaaaa taccattaaa aaatatctac aatgcactaa catccagaca gtgacggtgc
      301 ctgttcctgc gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga
      361 ttgcttatct caactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt
      421 cttgcctgtc tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc
      481 tatccgtttc cggaaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc
      541 tgaaagatat ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc
      601 aagagcaaac gacattctca cagattctat tagatgccag aatgcagcac gcaaaaaatt
      661 tgatacgcgt agaaggttca gtcaataaaa ttgccgaaca atgtggttat gccagtacat
      721 cttattttat ttatgcgttc cgcaaacatt tcggcaacag tccgaagaga gtttctaagg
      781 agtaccgttg tcaaagtcac acgggtatga atacgggcaa cacgatgagt gctttagcta
      841 tttgattatt tgctaacgag tagtcaacca cgcacgctac gtaagaatta tatggggcag
      901 ccattccctg ccccgcgttg tttttaggcg atatatttat tgaaataaat aagtgacatc
      961 catcacatat ttatgcactt gcataacctg ttgcatgatt atttatgatc tcaattcggc
     1021 attttgtcag taaaatgcaa taatttatta aatatcaata aattagttgt ttatcgtcga
     1081 gaaattactt aatagaacag aaagtaatgt caacgcttta tggactgtta tttccctttt
     1141 tttagctaaa tctgctatct ctttatgtga ctaacttcac ttacacccac ttatttctct
     1201 tcgtaaaatt acttcggaat taagtacaat aagaagagga acatttatga agtctgcatt
     1261 aaagaaaagt gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt
     1321 tgctgctcat gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc
     1381 agactttacg ccgactgaat acagtaccaa aggaaagcca aatattatcg tactaaccat
     1441 ggatgatctt ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat
     1501 ggaaaatcgt gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc
     1561 acaaaaatca acgccgacgc tcctttcatt aatggatgaa ggcgtacggt ttactaacgg
     1621 ctatgtggca cacggtgttt ccggcccctc ccgtgccgca ataatgaccg gtcgagctcc
     1681 cgcccgcttt ggtgtctatt ccaataccga tgctcaggat ggtattccgc taacagaaac
     1741 tttcttgcct gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca
     1801 cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa caaacgcgtg actatcatga
     1861 caacttcacc acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt
     1921 tatgggattc cacgctgcag gaacggcata ttacaactcc ccttcactgt tcaaaaatcg
     1981 tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt
     2041 tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc
     2101 gcacctgcca aatgataatc ctgcaccgga tcaatatcag aagcaattta ataccggtag
     2161 tcaaacagca gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat
     2221 tctcgaacaa ctgaagaaaa acggacagta tgacaataca attattctct ttacctccga
     2281 taatggtgcg gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag
     2341 tcagacctat cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca
     2401 acccggtaat tatgacaagc tgatttccgc aatggatttc tacccgacag ctcttgatgc
     2461 agccgatatc agcattccaa aagaccttaa gctggatggc gtttccttgc tgccctggtt
     2521 gcaagataag aaacaaggcg agccacataa aaatctgacc tggataacct cttattctca
     2581 ctggtttgac gaggaaaata ttccattctg ggataattac cacaaatttg ttcgccatca
     2641 gtcagacgat tacccgcata accccaacac tgaggactta agccaattct cttatacggt
     2701 gagaaataac gattattcgc ttgtctatac agtagaaaac aatcagttag gtctctacaa
     2761 actgacggat ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga
     2821 gatgcaaggc gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaggtaaa
     2881 tcaggagaag tttaacaata tcaagaaagc actaagcgaa gcgaaataac taaaccttca
     2941 tgcggcggat ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc
     3001 ctccagtttt caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca
     3061 gtttactcat gaaaaatgga tggatgacag cactctgaaa gagttcatca aacaatatat
     3121 cgcagcgtct ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg
     3181 cctggatttt ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat
     3241 ttttaatgca ttacaaacga atggcatttt attgaataat gaatggtgtg cctttctcaa
     3301 agaacatgaa tttctggttg gtatctcgat cgatggcccc caggagttac atgaccgtta
     3361 cagacgcagt aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct
     3421 gaaatcatat caagtagagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta
     3481 ccctcttgag gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga
     3541 attgctagaa accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg
     3601 tatcattgat ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat
     3661 gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt cagtttgaaa gctttgtcag
     3721 ccgttttttg gggaatgggc atacaagttg tattttccaa gagtcctgca aggataatct
     3781 ggttgttgaa agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa
     3841 aattggaaac attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca
     3901 aaaaaaacgg atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg
     3961 ttgtcctaag catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg
     4021 ttataaaatc cttttttcaa ccatggtacc ttatatgaac gccatggtag agttagctaa
     4081 gaacagagta ccgctttatc acattatgga tgttgcaaaa caaatggaga ataattaata
     4141 acacgccctc ttagctccag tcttagcctc cgtcttatga ccttaacaaa gcacccgaca
     4201 taaattcctg tcgggtgtgt cgtcatcatc atcttccctt gcgaaaatcc tggcatacgt
     4261 ggtatgcgtc agggcattag caataccctt gattatcaat agcataacca taaccactga
     4321 aaattaaaaa tttctatata tttcatattg ttaaatgaca cttaaataaa tttcatcagt
     4381 aaatcattgc catagataat cattggatgg cacaatcaat tttcgctaat gataaccatt
     4441 cccattacgt tttgtatgtt gatagcaaag tatctctgtc tgctgaagcc cttttggctg
     4501 cgtaagaata ataaaacttc agtgttgtta atcattatta tcctcgccat gatccttggc
     4561 gtggttaaaa ttcaggtctg gttaaacgac tggaataatg attttttcaa tgcgctaagc
     4621 cagaaagaaa ccgataagct ttggcagctt gtcctttggt tccctgccct gctggggatc
     4681 tttgtattaa tatccgtaaa caaaacatgg ttaattaaac tgctgactat tcgctggcgc
     4741 gaatggttaa cagattacta tcttaatcgg tggtttgcag ataaaaacta ctacttcacg
     4801 cagatctacg gtgagcataa aaatacagat aaccctgacc aacgtatcgc tgaagacatt
     4861 ctcttactga tcagcaaaac gctcagcctt tcatttggct tcatccagtc acttagcatg
     4921 ctgatcacct ttaccgttat tctttggcaa agcgcgggta cgctctcatt cactgttggt
     4981 ggaacagaat ggaatatcca ggggtatatg gtctataccg ttgtgctcat tgtgatcggt
     5041 ggaactttat ttacccataa ggtgggtaaa cgtattcgtc cgcttaatgt ggaaaaacaa
     5101 cgtagcgaag cgacatttcg aactaatctt gtgcagcata ataagcaggc agaactgatt
     5161 gcgctaagca atgctgagtc tctacaacgc caggagttgc gcgacaattt tcataccatt
     5221 aaagagaact ggcatcgttt aatgaatcgt cagcggtggc ttgattactg gcaaaatatt
     5281 tattcgcgct c
//
LOCUS       DAOFQD010000213         5057 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.214, whole genome shotgun sequence.
ACCESSION   DAOFQD010000213 DAOFQD010000000
VERSION     DAOFQD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.214"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1197)
                     /gene="opgB"
                     /locus_tag="QBF46_004596"
     CDS             complement(<1..1197)
                     /gene="opgB"
                     /locus_tag="QBF46_004596"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDQ6676693.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMN"
     gene            complement(1451..1945)
                     /locus_tag="QBF46_004597"
     CDS             complement(1451..1945)
                     /locus_tag="QBF46_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDQ6676694.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            complement(1994..2731)
                     /gene="dnaC"
                     /locus_tag="QBF46_004598"
     CDS             complement(1994..2731)
                     /gene="dnaC"
                     /locus_tag="QBF46_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDQ6676695.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            complement(2734..3273)
                     /gene="dnaT"
                     /locus_tag="QBF46_004599"
     CDS             complement(2734..3273)
                     /gene="dnaT"
                     /locus_tag="QBF46_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDQ6676696.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            complement(3380..3853)
                     /locus_tag="QBF46_004600"
     CDS             complement(3380..3853)
                     /locus_tag="QBF46_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDQ6676697.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            complement(3844..4614)
                     /locus_tag="QBF46_004601"
     CDS             complement(3844..4614)
                     /locus_tag="QBF46_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDQ6676698.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
BASE COUNT         1263 a         1262 c         1277 g         1255 t
ORIGIN      
        1 gttcatcgct aaatggtcag aagagacgac gatgacggta tctttaaacc acggtgacgc
       61 tttgattttg ttgataaacg ccgcgatatt ctcctggctg caacttaccg cgctgaatga
      121 ctggttcggc ttaccgtcaa aatcatattt tttgcggtta caggtacgcg agataaaacc
      181 atccgggtga tgggtatcga ctgtcagggt aaacagtgag aatcgctgac ctgagcggga
      241 aagctcttca aactttttcc acgcttcatc gagaacggta tcgtcgtaga atccccagtc
      301 gttgcgatag tgcgggtcgg ccaccacgct tttcagctct tctgagccgt ataagtggtc
      361 gaagccatgc gacttcagga acacatcttt accggcaaaa cgcagattcg cgccctgcac
      421 gaaatagttc tgataacccg agtttttcag gatatcgccc agacagatgt tctgcgggaa
      481 gaagctggag acagaggcgg aggcgttgcc ttcaaagggg gcaaacagcg gtatgccgca
      541 ctgagaagcc accatgcccg caatcgtgta atccgttcct ggcagctgct gcgtgtggct
      601 gaaatccagg ccttcatttt tcaacgcgcc cagttcaggc gtgagatccg ggaaagcttc
      661 gttatcaaaa taggtccgct cgagactttc gccgtagata taaaccaggt tgagtttcgg
      721 gtcagggata gttttcgacg gctctttata ataagccgca aagtccgggt cgccatcgcg
      781 tgactgggat ttcaccagtt ccgttatctg acgaaatgcc gggctggcat ccactgaacc
      841 cagcgccagt aagagtgcca gcaggctgta accaaaatgg tgcggatgat ggcgacgacg
      901 gcgcaggatc cagcccagcg caccgaacac cgccgtcagc cccagcacaa tgccgatacc
      961 cggaagaatg tatttgctga caccagcacc ggtcaggctg ttggttaagg tatagagaac
     1021 cgcgtcgtta ataccatcgc cagtaaaata gtcgctggca aacagggtga tatttaaaac
     1081 gacaaatagc cccagcaccg ttaacgtggc tgcaaaccac caggtgttac gtcccgcttt
     1141 ccatgcgtaa atcagcacag aggcgagaaa aagggcgaaa gagagtagtt ctgacaacgg
     1201 gcgatcctca ctaaaccggg ccgtttagcc aacgtttaag ctttttgaga agcacaatgt
     1261 aatggcgtcg ccatattgct gcaattctag tgtcaaaaaa atgcgatgtt gttaggaatt
     1321 agtttataaa cagacttttt tgattacggt cgcttcccgt gtaacagggg gaaaaccgtc
     1381 caggagcagg gaaaagggca ggagagagcc gcgtcagccg ggtaacagct aaaacgcggc
     1441 gtttcgcggt tcaggaaatg aagttcagcc cctgctttaa caccagatcg caggctttgg
     1501 ttttcacctt ttctgccagc ggagtcgttc cgatattatc cagattgagt tgctgaccat
     1561 ctttagtttt cagcaaaccc tgaatgccat ccagatagtt ggtgtcttct ttttgctctt
     1621 cactgttcag gcccagtttt tccagtacct gattcttgat gttttcggca tcggttaccg
     1681 acgccagctt ttgcttcgcg cagtattgca gaatgcctgc ggcattgttc atgttatctg
     1741 cgcttaaggc ctggtttccg ctgctaagca agttagttaa tgacgcgagc gaccaaccgc
     1801 cttcctgtgt cgtgctgttt tggttgccaa gttcgctggc ggcgctggag agcgcatctt
     1861 tccaggacgc agcatgcacc ccggtggaaa ttaatgcgct ggcggcaatg gcacagcaca
     1921 gaagatgttt aacagttttc atcatcatta ctcaaggtgg aattgtgtcg cagtataccg
     1981 ccagaaacgg ggattaatac tctttacctg ttacccggct acggtagcta tcccagttga
     2041 agatcaccca caaactgtta cccaggcgca tacggtccat cacgcgttcg cccagcaact
     2101 tggtcatctc ttccatatta ctgttggtca gcatcccggt tgggcgtttg gaagaagagc
     2161 ggcgatcgac gatctggttg atgatcactt tttcgtattt cgattcggtc tgcacgccga
     2221 tctcatcgat caccagcaga tcaacgttgc tcagatcgtt aagcagttgt tcttcactgg
     2281 taccgctatt cctgaaggta tctttcatcg ccgacataat atcggccacg gtgatgatca
     2341 atacggattt accgcgtagc agcagctcgt tgcagattgc cgccgccaga tggtttttgc
     2401 cggttcctgg cttaccagaa aagatgaagc tggcgatgtt gccgtcaaac tcttcgacat
     2461 actggcgcgc tttgcttaac gcattcatct gcccttcaca ctcaacgcga tagttctcaa
     2521 aggagcagtt ctgatgcagt ggacgaatac cggagcggtt aaaggtgcgc tgcattttca
     2581 tcgcccgatt ttcacgttcg agagcggcgg agcggatcgc cccttgttct ttctgccagg
     2641 ccagtagctc ttcacccgtt ttgaatgcag gtttgatatg ggcaggcatc attttttgca
     2701 ggcgttgcat caggtcgcca acgtttttca tcgttaccct ctgaatcctg gtggaatttg
     2761 gctgtcaggt tcgctgaccg tattcacatc tcgtttcggc agtccgccgt tgctggcacg
     2821 accgatttgc aggctgcgcg ccagtttttg ttgccactgc acatggtgaa agactttacc
     2881 ttccgcctgc cagtaggcaa tgaatgaggc cagttcttcg gtggtcaccg gctctcttag
     2941 cgccacgccc catagcgccg ccaggcggat aaagtcggca tcgggttgcc agtccggata
     3001 catggcgaat ttccccatgg ggacagcaac gggagcggct tgcggttcct gatagagttg
     3061 atcgtccaga gcgacatcgc ttcccggacg cgccagcttt tcttccagcg ccagcagttc
     3121 agccaggcgt gcaggcgtga cggcataaaa cgccggggca ttgttagcaa atacggcaac
     3181 cacaccgcct tcagcttttg ccagaacggt ttggtgatcg tgtaccaggg cgtcaatacc
     3241 aacgacgtcc ggggtcaaaa ctctggaaga catactgttt ctcaaatatg gaacgatgaa
     3301 cgagaaggga gataactttt atagtaacac acggaggaga ggaggctgtc tctcatccgt
     3361 gtgcttaagc gaggcaattt tatacgcgag ggcgcttgcg gtacaaccat aatccaggaa
     3421 tggaaagacc gatggataac gcaccaacaa tcgatgaagc tgtaagaaag ttggttaaca
     3481 gggtaatcat caacggttcg ctgtaaccta actggctgat ttttaccgcc gaaatcattg
     3541 cggtataagc cgatatgccc gggaacatag ggataacggc cgccacggta aacactttcg
     3601 gatgcgccag ataccagcgc gaccattgaa taccaatggt gccgaccagc atagaagcca
     3661 taaaagttga ccactcaata ttcagcccgc tggtcattaa gatcattcgt gaaccatgac
     3721 caatcgcgcc aagcagcgca caccagcgta acgcccgaac gggaacgttg aacaccatcg
     3781 caaagccgac cgcaggaatg gcggcgagga tcatatcctg cgccaacgct aacagaaatt
     3841 cgatcacacc catccgcgta gcccccaaat cgtcagtgcc atcactacgc cgacgcaggt
     3901 agccagtgtc agcagactgg cgatcgccca gcgtgccagt ccggtattga tgtggccttt
     3961 aaacatatcg gcgacggcat taatcaacgg aaatcccggc accagcagca gaacgctggc
     4021 ggccatcgcg atagtgggtg tattgctgaa agtgggaagt tgcaaaagca atccggaaat
     4081 ggtggtggcg gcgaaagcgg tgaggcaaaa gttgatctgc ggatgaagat gacgttgtgc
     4141 cagcagctgg cggatataca tcgcggccgt actggcaaag aaggtgatga cggcaccatc
     4201 ccagccacct ttattgagtt tgcagaaaca ggcgcaagaa aggccaacca ttaaggctac
     4261 cagccatctt gggtaacgta atggctgaat ttggctaaat cgtttctcta cgcctttgta
     4321 atccagcaga tggtgctccg ccagaatcac aatgtgctgg acttcagtca ccacatgcat
     4381 attaatgccg cgatcgtgat ttttacgtgt cgatgtcagg cattgcccat ctttaatggt
     4441 tgtcagcact atggcgttcg aagagataga actttcgacg ctgtccatcc ccaacgcccg
     4501 acccagtcgt gaggaaagct catcaaccaa cgcgctttcc gcaccatgtt gcaaaagaaa
     4561 taatccacac tggatacaaa gccgtgttac ggctcgctgt tgctcagttt gcataactcg
     4621 ccctttgtca tagacgtgct gcgtattgtt cagataatga gaatccattt gtatcatgaa
     4681 gcacggttgt tttgcggtag gttaagatca aaattgcgct gattaatcct ggttatcggg
     4741 acaagctcgg tgataaaaag cgaattatct ctcgtcgcaa acaaactatc acatgaatat
     4801 tatcaccata atgaatttat tgtttgtcct ttacgaacca gaataataga taaccgggca
     4861 cgttttttaa tgttaacaag ttaaaaccat cagatttcat tgaataaata ttggttgtgt
     4921 gatttttcat tctgcgggaa gggatatcat ttaattataa attaaatgaa tgtgatttct
     4981 gtttttcatt aagtattcct aacaacttaa tgtagatatt ttaaatgtct ccaggctatt
     5041 tcaccaggtt atcattt
//
LOCUS       DAOFQD010000214         5013 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.215, whole genome shotgun sequence.
ACCESSION   DAOFQD010000214 DAOFQD010000000
VERSION     DAOFQD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.215"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..218
                     /locus_tag="QBF46_004602"
     CDS             <1..218
                     /locus_tag="QBF46_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6676699.1"
                     /translation="MSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYI
                     ECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            245..523
                     /locus_tag="QBF46_004603"
                     /pseudo
     CDS             245..523
                     /locus_tag="QBF46_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            536..1039
                     /locus_tag="QBF46_004604"
     CDS             536..1039
                     /locus_tag="QBF46_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6676700.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSVVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            1099..2013
                     /locus_tag="QBF46_004605"
     CDS             1099..2013
                     /locus_tag="QBF46_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6676701.1"
                     /translation="MGKTISIKVLFGIYFLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLIRNGKILAAGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            2347..4626
                     /gene="ydeP"
                     /locus_tag="QBF46_004606"
     CDS             2347..4626
                     /gene="ydeP"
                     /locus_tag="QBF46_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDQ6676702.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMEIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKNGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4874..>5013
                     /gene="safA"
                     /locus_tag="QBF46_004607"
     CDS             4874..>5013
                     /gene="safA"
                     /locus_tag="QBF46_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDQ6676703.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     IS"
BASE COUNT         1380 a         1101 c         1199 g         1333 t
ORIGIN      
        1 gtatgagcgc aaaaggttgc tgctacgata atgcctgcgt ggaaagcttc tttcattcgc
       61 tgaaagtgga atgtatccat ggagaacact ttatcagccg ggaaataatg cgggcaacgg
      121 tgtttaatta tatcgaatgt gattacaatc ggtggcggcg gcacagttgg tgtggcggcc
      181 tcagtccgga acaatttgaa aacaagaacc tcgcttaggc ctgtgtccat attacgtggg
      241 taggatcagg gttgcattta acggtattga ggatgcagaa aataatactc tgttgaaact
      301 ggatgaagga agcaatacgg cttccggttt gggtatagaa atactggacg gaaatatgcg
      361 tccggtgaaa ttgaatgacc ttcatgccgg gatgcagtgg atcccactgg taccagaaca
      421 gaacaatatt ttgccttact ccgctcgtct gaagtcaact cagaagtccg tcaatccggg
      481 actggtgagg gcttcggcaa cctttaccct tgaatttcaa taatcagagt cagatatgaa
      541 aagacttcac aagaggttcc tgttagctac gttttgcgcg ttattcacag caactctcca
      601 ggccgccgat gtcactatca ctgttaatgg tcgggtagtc gctaaaccct gcactattca
      661 aaccaaagaa gctaacgtta atctcgggga tctttatacg cgcaatctgc aacaacctgg
      721 ttctgcatct ggctggcaca atattactct gtcattaacc gattgtccgg ttgaaacaag
      781 tgtagtgacg gcaatcgtga caggttcaac tgacaatacg ggttattaca aaaatgaagg
      841 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgcgacgt taaaaaatgg
      901 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      961 aagagctatc acggtgaatg gaaacgcaag ccagggaacg atcgaggcgc taatcaatgt
     1021 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
     1081 ttaaccttag gatatatcat gggtaaaaca atcagtataa aggtcctgtt cggtatttat
     1141 tttctattaa tggcaggtaa agtttttgcc ttcagttgta atgtcgatgg cgggtcaagt
     1201 attggagctg gtacaacctc tgtttatgtt aatctcgacc ctgtaataca gccgggccag
     1261 aatctggttg tagacttgtc tcagcatata agttgctgga atgattacgg cggctggtac
     1321 gacactgatc atataaacct ggtacaaggc tcagctttcg caggctctct acagtcatat
     1381 aagggatcgc tgtactggaa taatgtgacc taccccttcc cgttgacgac aaataccaac
     1441 gtattggata ttggtgataa gacaccgatg ccattaccgt taaaactcta tattacccca
     1501 gtcggggcgg ccggtggagt tgtcataaaa gccggggaag ttattgcccg tattcatatg
     1561 tataaaattg ccacgttagg aagcggaaat ccgcgtaatt ttacgtggaa tattatttca
     1621 aataacagtg ttgttatgcc aacaggcggc tgcactgttg atagtcgtaa tgtcaccgtg
     1681 gatctcccgg actttcctgg aagcgcagaa ataccccttg gcgtatattg ctcaagcgaa
     1741 caaaagctct cattttatct ttcgggtgcc accactgaca gtgcccgaca agtgtttgct
     1801 aacacggcac ctgatgcaac aaaagcatcg ggagtgggtg tctctctgat acgtaatgga
     1861 aaaatactgg ctgcgggaga aaatgtctcc ctggggacag taaataaaag caaagtacct
     1921 ctgggtttaa gcgcaaccta tggacaaaca ggaaacaagg tttcagccgg aactgtccag
     1981 tcagtcattg gtgtcacttt tatctatgaa taattgtgat tttgacctta gtctatgcgc
     2041 tccattcacg aaaattgcgc ggataattat tttgtgaagg ctattatcct acacctgtaa
     2101 gaaaatccgc gcacaattga agccagagtc ttatttttta tattgaaaaa tatgttaatt
     2161 taaacactat gagagatgat gcttaactta tcgtatttaa tctattgttt aacgataata
     2221 gattaaaaat aggaagagag tagtccccta ctcttatata tccatgttgg cgataatccc
     2281 tttgtatata ctgtgcatca tcgctattac aaatcctaat aattcatttc cacacaggat
     2341 aagtagatga agaaaaaaat tgaatcctac cagggtgctg ccggtggttg gggtgctgtt
     2401 aaatccgtag cgaatgcagt acgtaagcag atggagatac gccaggatgt tattgccatg
     2461 tttgacatga ataagccaga gggctttgac tgtccgggtt gtgcatggcc agatcctaag
     2521 cacagtgcgt cattcgacat ttgtgaaaac ggcgcaaaag caatcgcctg ggaagtcacg
     2581 gataagcagg taaatgcctc tttctttgct gagaatacgg ttcaatcatt acttacctgg
     2641 ggagatcacg agcttgaggc tgcgggacgt ctcactcagc ctttgaaata tgatgccgtc
     2701 agcgactgtt acaagccatt aagctggcaa caagctttcg acgaaattgg cgcacgcctt
     2761 caaagctata gtgatcccaa tcaggttgaa ttctatactt cgggccgcac ttccaatgaa
     2821 gctgcctttc tttatcagct ttttgcccgt gaatacggga gcaataactt tcccgactgc
     2881 tccaacatgt gccatgaacc gacaagcgtg ggtttggcag cgagtatcgg tgtaggtaaa
     2941 gggaccgtgt tgctggaaga ctttgagaag tgcgatttag tcatttgcat tgggcataac
     3001 cctggtacaa accaccctcg catgctgact tcgttgcgcg ctttagtgaa acggggagcg
     3061 aaaatgatcg ccatcaatcc tctacaggaa cgtggcctgg agcgatttac cgcaccgcaa
     3121 aacccgtttg aaatgctgac gaactctgag actcagttgg ccagtgccta ctataacgtg
     3181 cgcattggtg gcgatatggc gttgctcaag gggatgatgc gcctgttaat tgagcgcgat
     3241 gatgctgcaa gcgccgcagg tcggccttca ttgcttgatg acgagtttat tcaaacgcat
     3301 accgtcggct ttgacgagct acgccgtgac gttctcaatt ccgagtggaa agatatcgaa
     3361 cgtatttctg gactaagtca gacacaaatc gccgaactgg ctgacgcata tgccgctgcc
     3421 gaacgcacca ttatctgtta cggaatgggg atcactcagc acgaacatgg tacccagaac
     3481 gtacagcaac tggtcaatct gctgttgatg aaaggtaaca ttggcaagcc tggtgcgggt
     3541 atctgcccac tacgtggaca ctctaatgta cagggcgacc gaaccgtcgg tatcaccgag
     3601 aaaccgtctg cagagtttct ggctcgtctg ggtgagcgct atggcttcac cccacctcat
     3661 gcacctggac atgctgcaat tgccagcatg caagcaatat gtacggggca ggctcgagca
     3721 ttgatctgca tggggggcaa ttttgcgctg gcaatgccag atcgggaagc gagcgctgta
     3781 ccgttaacgc aattagattt ggcggtacac gtagccacta agcttaaccg ctctcatctg
     3841 ttgaccgcac ggcatagcta tattctgccg gtcctgggac gtagcgagat tgacatgcaa
     3901 aaaaacggtg cgcaggcggt aaccgttgag gattcaatgt cgatgattca tgcctcgcgt
     3961 ggcgtgttaa aacccgccgg tgtaatgctg aaatcagagt gtgcagtggt cgcgggaatc
     4021 gcgcaggcag cactacccca gagcgtggta gcctgggagt atctggtgga agattatgat
     4081 cgcattcgca atgacattga agctgtgctg ccagagttcg ccgactataa ccaacgcatc
     4141 cgtcatcccg gtggttttca cctgataaat gcagctgctg aaaggcgctg gatgacgccg
     4201 tcaggtaagg ctaatttcat taccagcaaa gggctgttag aagatccctc ttcagcgttt
     4261 aacagtaagc tggtcatggc gacagtacgc agtcacgatc agtacaacac gacgatttat
     4321 ggtatggatg atcgctatcg aggggtattc ggtcaacgag atgtggtctt tatgagtgct
     4381 aaacaagcta aaatttgccg tgtaaaaaac ggcgaaagag ttaatcttat tgcgcttacg
     4441 ccagacggta agcgcagctc acgccgcatg gatagattaa aagtggtcat ttaccctatg
     4501 gctgaccgct cactggtgac ctattttcca gaatcgaatc acatgctaac tcttgataac
     4561 cacgatccat taagtggcat tcctggttat aaaagtattc cggttgaatt agaaccatca
     4621 aattaattcg cctcctcatt tcttctgctg tcatccgcac agcagaagaa tttctcattg
     4681 actattatcg cgcaatttgc tcacattgat taaattaaac cacacactat aagatataaa
     4741 cctctgccta cagctgtaag aaactccgct cagtactgaa gtgccagtcc tatttcctct
     4801 tttctctagc ctgttatatt aagcatactg attaacgatt tttaacgtta tccgctaaat
     4861 aaacatattt gaaatgcatg cgaccacagt gaaaaacaaa atcacgcaaa gagacaacta
     4921 taaagaaatc atgtctgtaa ttgtggttgt cttattactg acccttacgt tgatagccat
     4981 tttttcggca attgatcagc tgggtatttc aga
//
LOCUS       DAOFQD010000215         4979 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.216, whole genome shotgun sequence.
ACCESSION   DAOFQD010000215 DAOFQD010000000
VERSION     DAOFQD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.216"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1172
                     /locus_tag="QBF46_004608"
     CDS             <1..1172
                     /locus_tag="QBF46_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDQ6676704.1"
                     /translation="NIDQIFSELPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSA
                     ALPFVIGGGILVALANMLVQFGLPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYI
                     ASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGK
                     ALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVG
                     AMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTR
                     QEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPG
                     LGIFDPLIGLMSPVDSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            1162..2232
                     /locus_tag="QBF46_004609"
     CDS             1162..2232
                     /locus_tag="QBF46_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDQ6676705.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIALDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            2232..3980
                     /locus_tag="QBF46_004610"
     CDS             2232..3980
                     /locus_tag="QBF46_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDQ6676706.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNSVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNCITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4030..>4979)
                     /locus_tag="QBF46_004611"
     CDS             complement(4030..>4979)
                     /locus_tag="QBF46_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDQ6676707.1"
                     /translation="SQPAAPAPEAKPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDV
                     YIKCYNKLQIPVQRSLARYADWLKDFKQGPTGEERTVYGIYGISESNLAECEKGVKSA
                     VALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKN
                     LEAFEPVAESYHAAIQEINDKRQLAELKNIEEREGRTFHYYSLAVMISAKQINNLISQ
                     DEFDAEAAMKKVSELETLVAQAKEADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPY
                     SDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNRLR"
BASE COUNT         1129 a         1269 c         1283 g         1298 t
ORIGIN      
        1 aaaatatcga ccagattttc agcgaattac cgacaaactc gcagcttttt gccgcagata
       61 gcggcgtgaa gcttggcaaa caggaagtgc aaagcggcag cgtgatgagc cacctgatgg
      121 cgggcgtctc tgcggcgctg ccgtttgtca tcggcggtgg tatcctggtg gcgcttgcca
      181 acatgctggt gcagttcggt ttgccgtata ccgatatgtc gaaaggcgcg ccgtcgttta
      241 cctgggtagt tgaatccatc ggttaccttg gcttcacctt tatgatcccc atcatgggcg
      301 cttatattgc ctcgtcgatt gccgataaac cggctttcgc tccggcgttt ctggtttgct
      361 atctggcgaa cgacaaagcg ctgctcggca cccagtcggg tgcgggattc ctcggcgcgg
      421 tggtgctggg gctggcgatt ggctatttcg tcttctggtt ccgcaaagtg cgccttggca
      481 aagcattaca accgctgctc ggttcaatgc tgatcccgtt cgttactctg ctggttttcg
      541 gcgtgctcac ttactacgtt atcgggccgg tgatgtccga cctcatgggc gggctgctcc
      601 acttcctgaa taccattcct ccatcaatga agtttgcggc agcgtttctg gtcggtgcga
      661 tgctggcgtt cgatatgggc ggccccatca ataaaaccgc ctggttcttc tgcttctcac
      721 tgctggaaaa acacatttat gactggtacg ccatcgtcgg cgttgtcgcg ttgatgccgc
      781 ccgttgcggc gggtcttgcg acgttcattg cgcccaaact gtttacccga caggaaaaag
      841 aggctgccag tagtgccatt gtggtcggtg ccacagtcgc aaccgaaccg gctatacctt
      901 acgctctggc agctccgttg ccgatgatta ccgccaatac gctggcgggc ggcattaccg
      961 gcgtgctggt gatcgctttc ggaatcaaac gtctggcacc tggtttgggt atctttgacc
     1021 cgctgatcgg cctgatgtcg ccggtagatt cgttttatct ggtgctggcg attggcctcg
     1081 cgctgaacat ttcattcatt atcgtcctga aaggattgtg gttacgccgc aaagcgaaag
     1141 ccgcgcaaca ggagcttgtc catgaacatt gagttactgc aacagttgtg cgaagccagc
     1201 gccgtcagcg gcgatgaaca ggaagttcgc gacattctga taaacacgct ggaaccttgc
     1261 gttaatgaga tcacctttga tggtctgggc agctttgttg cccgtaaggg gaataaaggt
     1321 ccaaaagttg ccgttgtcgg gcatatggat gaagtcggct ttatggtcac ccacatcgac
     1381 gagagcggtt ttctgcgctt taccaccatt ggcggctggt ggaatcagtc aatgctcaac
     1441 caccgggtaa ccatacgcac acacaaggga tttaaaatcc ctggtgtgat tggttccgtc
     1501 gcacctcatg cattaaccga aaagcaaaag caacagccgc tgtcatttga tgagatgttc
     1561 attgatattg gcgcgaacag tcgcgaagag gtggaaaagc gcggcgtgga aattggcgat
     1621 tttattagcc cggaggccaa ttttgcctgc tggggcgaag ataaagtagt cggcaaggcg
     1681 ctggataatc gcatcggctg cgcgatgatg gctgagctac tacagacagt aaataacccc
     1741 gaaattacgc tgtacggcgt cggcagcgtg gaagaagaag ttgggctacg cggggcacaa
     1801 acctcggcgg aacacattaa accggatgta gtgatcgcgc tggataccgc tgtcgcaggc
     1861 gatgttccgg gcattgataa cattaaatac ccgctgaaac tgggccaggg gccggggctg
     1921 atgctgtttg acaagcgcta cttccccaac cagaaactgg tagcagcgtt aaaaaactgt
     1981 gccgcacata acgatttacc gctgcaattt tccaccatga aaaccggagc gacggatggc
     2041 gggcgctaca acgtgatggg cggcgggcgt ccggttgttg cgctgtgcct gccaacccgc
     2101 tatctgcacg ctaacagcgg catgatttca aaagccgatt acgatgctct gctcacgttg
     2161 atacggggtt ttctgacgac cttaaccgcg gagaaagtca acgcgtttag ccagttccgt
     2221 caggtggatt aatgctaaac gaacgccagt taaagattgt cgatctgctg gagcaacagc
     2281 cgcgcacgcc tggtgagctg gcgcaacaga ctggcgtttc aggcaggacc atcctgcgtg
     2341 atattgacta tctcaacttt acccttaacg gcaaagcccg cattttcgcc agtggcagtg
     2401 cgggctatca gctggaaatc ttcgagcgcc gcagcttttt tcagttgctg caaaagcacg
     2461 ataacgacga tcggctgctg gcgctgttat tactgaatac tttcactccc cgtgcgcaac
     2521 tcgcctcggc gcttaatttg ccagaaacgt gggtagcaga gcgtctgccc cggttaaaac
     2581 agcgttatga acgcacttgt tgcctggcca gccgccctgg tttgggccat ttcattgatg
     2641 agacagaaga gaaacgcgtt atcttgctgg cgaacttgct gcgcaaagat ccgtttttaa
     2701 ttccgctggc gggcataaca cgagacaacc ttcagcattt atccacggcc tgcgacaacc
     2761 aacaccgctg gccgctcatg cagggtgatt atctctccag cctgattctg gcgatttacg
     2821 ccctgcgtaa tcaactgacc gatgagtggc cgcaatatcc cggtgacgag ataaaacaaa
     2881 tcgttgaaca tagcggtctg tttctcggtg ataattctgt aagaaccctg acgggtttga
     2941 tagagaaaca gcatcagcaa gcgcaggtaa tttcagccga tcatgtgctg gggttgctgc
     3001 aaagggtgcc aggcatcgcg tcattgaata ttattgatac gcagctggtt gagaatatta
     3061 ccgggcattt attacgttgc cttgccgcac cggtgtggat tgctgagcac cgccaaagca
     3121 gcatgaataa cctgaaagca gcctggcctg cggcctttga tatgagtctg cactttatta
     3181 cgctactgcg tgaacagctc gatattcccc ttttcgacag cgatctgatc ggtttgtatt
     3241 ttgcctgtgc gctggaacgg catcaaaacg aacgccagcc gatcattttg ctctcggacc
     3301 agaacgcgat tgccactatt aatcagctcg ccattgagcg cgatgtttta cattgtcggg
     3361 tgattattgc ccgtagctta agcgaacttg ttgccattcg cgaagagatt gagccgttat
     3421 tgatcattaa caacagccat tatttactgg atgacgcggt aaataattgc atcaccgtaa
     3481 aaaatatcat tacggctgcc ggtatcgaac aaataaaaca ttttctggcg acggcattta
     3541 ttcgccagca accggagcgt tttttctctg cccccggaag ttttcattat tcgaatgtac
     3601 gcggtgaaag ctggcaacat attacccggc aaatttgtgc gcaattagtc gcacaacacc
     3661 atattaccgc cgatgaagcg caacggatca tcgcccgcga aggcgaaggt gaaaacctga
     3721 ttgttaatcg cctcgccatc ccacattgct ggagcgaaca ggagcgacgt tttcgtggat
     3781 tttttattac cctcgcccaa ccagttgagg tgaataacga agtcattaac catgtattga
     3841 tcgcctgcgc cgccgccgat gcgcgtcacg agctaaaaat atttagctat ctggcaagcg
     3901 tattgtgtca gcatccggca gaggttattg ccgggttaac aggatatgag gcatttatgg
     3961 agttacttca caaggggtga actgtgtcgg atgcgccgca tccgacactg catttatcat
     4021 caaaaacgtt taacgcaggc gattataatc atcgaccatt tcgttgtact cgcgtaatgc
     4081 tctcgggaaa gagtcttcga ccatccagct tgagtttgcg tcctgaagtt gctctttatc
     4141 ccagtcagag tacgggactt tatctctgat gcggcgaacg tattttttag cctcgagttg
     4201 atactggcct gccgaattaa taaacgagaa attcatgccg cctttatccg cttctttggc
     4261 ctgcgccacc agcgtttcca gttcagacac tttcttcatt gccgcttctg catcaaactc
     4321 atcttgcgat atcaggttat taatttgttt cgctgaaatc atgactgcca gagagtagta
     4381 gtggaatgtt ctcccttctc tttcttcaat atttttcagc tccgcaagct ggcgcttatc
     4441 attaatttcc tgaatcgccg catgataaga ttctgctaca ggttcaaagg cttccagatt
     4501 ttttaagaat gtctggtgca gcgttttccc tttcgcaaac gcatcgtctt tataattttc
     4561 ctgggtgtaa tatttatcca tttcgttaat ggtattaccc aacgccacgg cagcatcgat
     4621 ataactcacc gccacgccat caattggttg cagcgcaggc gttaacgcca cagcactttt
     4681 tacgcctttt tcacactcag cgaggttgga ttcactaatg ccgtagatgc cataaacagt
     4741 acgctcttca ccggtaggcc cctgtttaaa atctttcagc cagtcagcat aacgcgccag
     4801 actgcgctgt accgggatct gtaacttgtt gtagcacttg atatagacat ccattttttc
     4861 gtcgaaaacc gcttgttcat cgaccaccgg ttgcgcaggt gtttcaggtt tagcttctgc
     4921 ttttgcaacg ggagcttcag taggtttcgc ttcaggagct ggtgcggcag gttgactat
//
LOCUS       DAOFQD010000216         4978 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.217, whole genome shotgun sequence.
ACCESSION   DAOFQD010000216 DAOFQD010000000
VERSION     DAOFQD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.217"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..2970)
                     /locus_tag="QBF46_004612"
     CDS             complement(<1..2970)
                     /locus_tag="QBF46_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024945956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDQ6676708.1"
                     /translation="MARRQILSLSERESLLALPDDELTLTRMAYFSEHDLALISAHRK
                     PASRFGFAVLLCYLKNVGFAPDKKIPPSDALLKHIASRLKLTGDLWPAYLSGRDTTRR
                     EHLTELYRYLGVKAFTGKIQQDGITHLLSMATRTDKGILLAEELLVWLRQNNVIIPAI
                     DVVERTCAEAMAGGDKIVFQTLNAPLTPAHRDALDRLLESSDNQPSRLTWLLQPPGKI
                     NGKNVLQHLDRLSSIESLALPEGIDRTIHQNRLLKLAREGRKMSSRDLTRFSAARRHA
                     ILVCVLEEARATLTDEVIELHERMLNSLFNKAKRTQAERLQQTGKLIQSKLRQYIDVG
                     QALSDARDSGGDPWLAIENILPWPEFVASLEETRHLARKNNFDPLHIITEKYSTLRKY
                     APRMLSALQLVATPAAQPLADALVVIKDMYRKQSRKVPATAPLEFVPESWRKVVITPA
                     GIDRQYYEFCALSELKGALRSGDIWVKGSRRYKNFDDYLIPEKDFDKLTPALPLPVSA
                     DYHEYIASRMTLLQSRLEEVNAMAALGELPDVEISDKGVKVSPLDNCVPAQVSPLAEL
                     VYSMLPRPKITEILDEVNCWTTFTRHFSHIKNDITRPDTRQLLTTILADGINLGLTKM
                     AEACPGSTKSSLEDIQAWYTRDETYSAALAELVNAQGKRPLAAFWGDGTTSSSDGQNF
                     RTGSSGRYAGQVNPKYGQEPGRQFYTHISDQYSPFYTCIISRVRDSTHVLDGLLYHES
                     DLEIREHYTDTAGFTDHVFALMHLLGFAFCPRIRDLHDKKLFIKGKADQYPALQSLIS
                     TTSLNLKEIEIHWREVLRLATSIKQGTVTASLMLKKLASYPKQNGLAKALREIGRIER
                     TLFMLDWFRDPALRRRVQAGLNKGEARNALARAVFLHRLGEIRDRKPENQSYRASGLT
                     LLTAAISLWNTIYMERAVDALKRKGMKINERLLSHLSPLGWEHINLTGDYIWKSNRIP
                     ASGKFRRLRPAKVGRSKN"
     gene            3138..3779
                     /locus_tag="QBF46_004613"
     CDS             3138..3779
                     /locus_tag="QBF46_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6676709.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQERGHKIASYYRENISG
                     TKLERPELGRLLMDSHHNDILLVEQIDRLTRLSNSDWMTLKKQIEQHELRIVSLDVPT
                     SWQALSDKDPSQADPITRAVITAINNMLIDLMAAMSHKDWLSRRQRQKQGIERAHTIG
                     KYRGKQADQERHQKVLYYRQVKKLSIRETADATGYSASQVCRIQALHKNNETA"
     gene            complement(4036..4959)
                     /locus_tag="QBF46_004614"
     CDS             complement(4036..4959)
                     /locus_tag="QBF46_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDQ6676710.1"
                     /translation="MTKTKGLPRPLTHYAWLSIATAIATIGLKGVAWKMTGSVGLLSD
                     AIESVVNLAGALMALWMLTLAALPADENHAYGHGKAEYFSSAFEGFLILLAAASIAYT
                     AVERMLTPQPLEEIGLGLLVSTVASILNFVTARILLRAGRQHNSITLEADAHHLLTDV
                     WTSVGVIFGVGLVYLTGWFWVDPIVALLVAANIVWTGYQLMSRSAAGLMDVSLPTEEL
                     KKIESLLAGYREQGLDFHALRTRQAGGRAFMTMHILVPGRWTVQYGHDWAERIENDIR
                     TALPFIHITTHVEPLEDPASMNDQTLDISDH"
BASE COUNT         1232 a         1259 c         1277 g         1210 t
ORIGIN      
        1 gttttttgac cttcctactt tagctggccg cagccgacga aacttaccgg aagccggtat
       61 ccggttgctt ttccagatat aatcgcctgt aagattgatg tgttcccagc ctagcgggga
      121 caaatgcgat agcagtcgct cgttgatctt catcccttta cgcttcaggg catcgaccgc
      181 tctttccata tagatggtat tccacagcga gatagctgcc gtcagcagcg tcagtccgct
      241 ggcgcggtag ctctgatttt ccggtttacg atccctgatt tcacccagac ggtgcaggaa
      301 taccgcgcgc gccagcgcgt tacgggcttc tcctttattc agtcccgcct gtacccgccg
      361 acgaagtgcc ggatcgcgga accagtcgag cataaacagc gttcgttcaa tacggccaat
      421 ttccctcagt gctttggcaa gtccgttttg cttggggtag ctggcaagct ttttaagcat
      481 cagggatgcc gtcacggttc cctgctttat cgaggttgcc aggcgcagca cttcacgcca
      541 gtgaatttcg atctctttca gattcaggct ggttgttgat atcagtgact gaagcgccgg
      601 gtattggtct gctttccctt tgataaacag ctttttgtcg tggagatccc tgattcgtgg
      661 acagaacgca aagcctagca gatgcatcag ggcaaagaca tgatcggtga aaccagctgt
      721 atcggtgtaa tgctccctga tttccaggtc gctttcgtga tacagcaaac catcgagaac
      781 atgggttgaa tccctgaccc gacttattat gcaggtgtaa aacgggctgt attgatccga
      841 aatatgggta taaaactgac gtccaggttc ttgcccatat ttcgggttaa cctgccctgc
      901 gtagcggcct gagctgcctg ttctgaaatt ctgtccatct gatgatgacg ttgttccgtc
      961 tccccagaat gccgccagtg gtctttttcc ctgcgcattt accagctcag caagcgcggc
     1021 tgaataggtt tcgtcgcggg tataccatgc ctgaatatct tcgagtgatg atttggtgct
     1081 cccggggcag gcttctgcca ttttggtcag gccaagattg atgccatccg caagaatggt
     1141 ggtgaggagc tggcgggtat cgggacgggt aatgtcattt tttatatgcg aaaaatgacg
     1201 ggtaaacgtc gtccagcagt ttacctcgtc gagaatttcc gtgattttag gtcgcggtag
     1261 catgctgtaa accagttctg ccagcggtga aacctgtgcc ggaacgcagt tatccagcgg
     1321 agagactttt acgcccttgt cggatatttc cacatcgggc agttcaccaa gggcagccat
     1381 ggcattgacc tcttcaagtc tggactgaag cagagtcata cggctggcaa tgtactcatg
     1441 ataatcagca gatacgggca gtggcagcgc cggtgtgagt ttgtcaaagt ccttttcggg
     1501 aatgaggtaa tcatcaaaat ttttgtagcg gcgtgagcct ttaacccaga tgtccccgga
     1561 gcgcaacgct cccttaagct cgctgagcgc acaaaattca taatactgcc ggtcaatacc
     1621 cgcaggtgta atcaccacct tgcgccagct ttcagggaca aactcaagcg gcgcagtcgc
     1681 cggaacttta cgtgactgct tccggtacat atccttgatc acaaccagcg catcagccag
     1741 aggttgtgct gccggggttg cgactaactg taaagcggac aacatgcgcg gggcatattt
     1801 ccgtaatgtg ctgtatttct cagtaataat atgcagcggg tcaaaattat tttttctggc
     1861 gagatggcgt gtttcctcca gactggcgac aaattcaggc cacgggagta tgttttctat
     1921 tgcgagccat ggatcgccac cggagtcgcg ggcatcagac agcgcctgac caacatcgat
     1981 gtactgcctc agtttggact ggatcagctt tccggtctgc tgaaggcgct cagcctgtgt
     2041 tcttttggct ttgttgaaaa ggctgttcag cattcgttca tgcagctcaa tcacttcgtc
     2101 tgtcagtgtg gctctggctt cttccagcac gcagaccagg atcgcatgac ggcgggctgc
     2161 tgaaaatcgg gtcaaatccc ggctactcat ctttcggcct tcccgcgcca gtttcagcaa
     2221 ccggttctgg tgaatggtac gatctatacc ttcaggtaat gccagcgatt caatgctgct
     2281 gagccgatca agatgttgca gtacgttctt accattgatt ttacccggcg gttgcagaag
     2341 ccatgtcagc ctggaaggct gattatctga tgactcaagt aaacgatcca gggcatcccg
     2401 gtgcgccgga gttaacgggg cattcagggt ctgaaatacg attttatctc cgccagccat
     2461 ggcctccgca caggtccgct ccacgacatc aatcgcggga attatcacgt tgttctgccg
     2521 gagccagacc agtaactctt cggccaggag aataccttta tcggtgcgcg tcgccatcga
     2581 caacaggtgc gtaataccgt cctgctgtat tttgccggtg aacgccttta cgccaagata
     2641 gcggtacagt tcggttaaat gctcacgccg ggtcgtatcc ctgccggaaa ggtaagcagg
     2701 ccagagatcc ccggtaagct ttagcctgct ggcgatatgc ttcagcagcg catcagaggg
     2761 tgggattttt ttatccggag caaagccgac atttttcagg tagcaaagaa ggacggcaaa
     2821 accaaaacgg ctggcaggtt tacggtgagc actgataagt gccagatcat gctcactgaa
     2881 ataggccatt cgggtcagcg ttaactcatc atctggcaat gccagtaatg attctctttc
     2941 cgacagagaa agaatctgcc tccttgccat atagtttccc tcataaatta ttcggttcgg
     3001 tatttttaac aagaatgctt atcgcataag ggtacacctt gacgccacaa ccaaaacgtg
     3061 acggcacacg ataaatgaca taacctatat gcgatagaat aaatgcaata acctaaatgc
     3121 gataacgcgg tgggaaaatg ttcatcagag cttatttaag ggcatcgacg gaagaccagt
     3181 tcgccgatcg ggcaaaagag atgctggagc agttcgtcca ggaacggggg cataaaatcg
     3241 ccagctacta ccgggaaaat atcagtggca caaaacttga gcgcccggaa ctagggcgct
     3301 tactgatgga cagtcaccac aatgatattt tattggtcga gcagatagac cgtctgacac
     3361 gtctcagcaa cagcgactgg atgacgctaa aaaaacagat agaacaacat gagctgcgaa
     3421 ttgtcagtct tgatgtgccc acatcatggc aggcactgtc agataaggac ccttcgcagg
     3481 ccgacccgat aacccgcgca gtaataaccg ccatcaataa catgcttatc gatctgatgg
     3541 ccgcaatgtc acataaggac tggctgagcc gccgccagcg gcaaaaacag ggaatcgagc
     3601 gggctcatac aattggaaag taccggggta agcaggccga tcaggaacgg catcagaaag
     3661 tcctgtacta ccggcaggta aagaaactga gtatccggga aacggcggat gctacaggct
     3721 acagtgcttc acaggtttgc cggatacaag cgcttcataa aaataatgaa actgcctgaa
     3781 aggctggaat aacggtgttt atttgcagtc atggcctaat agttagcgcc ctgtaacaac
     3841 ttttgtgatt agcagcctga cgcatcatct atgtaaggaa agtccgctgt gtgccagaag
     3901 cggacttttt aaaaatattg cgtgcccaac aattgggcgc aggtcacatc aagtaccctc
     3961 agaattatag agaatacctg aaatttttgt agagcgaggt ttgttaccct gcccgaccag
     4021 cgacatgtgg ttagtttagt gatcagaaat gtcgagcgtt tggtcgttca ttgacgcggg
     4081 atcttccaac ggttccacat gagtggtgat atggataaaa ggcagtgcgg tgcggatatc
     4141 attctctata cgctcggccc agtcgtgccc atattgaaca gtccatcgcc caggaactag
     4201 gatgtgcatt gtcataaacg cccgcccgcc agcctggcgt gtacgtagtg catggaaatc
     4261 aagcccctgt tcacgatatc ctgccagcag tgactcgatt tttttgagtt cttccgtggg
     4321 tagcgatacg tccatcagac ctgcagctga acgactcata agctgataac cagtccaaac
     4381 gatgttggct gcgaccagca atgcaacgat cggatcgacc caaaaccagc cggtcagata
     4441 aaccagtccg acaccaaaaa tgacaccgac cgacgtccag acatcggtca gcaggtgatg
     4501 agcatctgcc tcaagagtga tagagttgtg ctgcctgcca gcccttaaca aaatgcgagc
     4561 cgtcacaaaa ttaaggattg atgcgactgt tgaaaccagt aatccgagac caatctcctc
     4621 aagcggctgt ggagttaaca tccgctcaac tgcggtatag gcgatactgg ctgccgccaa
     4681 taggatcaga aatccttcga aagcactcga gaaatattcg gctttgccgt gcccatatgc
     4741 atggttctca tcggccggaa gcgcagccaa ggtcagcatc cagagcgcca ttagggctcc
     4801 tgcgaggtta actacggatt cgatggcatc agatagcaga ccgaccgaac cggtcatttt
     4861 ccacgccaca cctttgagtc cgatagtggc aatagccgtg gcaatggaaa gccaagcgta
     4921 gtgcgtcagc ggacgaggta acccttttgt tttagtcatt attaacttcc taaaatag
//
LOCUS       DAOFQD010000217         4974 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.218, whole genome shotgun sequence.
ACCESSION   DAOFQD010000217 DAOFQD010000000
VERSION     DAOFQD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.218"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..932)
                     /gene="tuf"
                     /locus_tag="QBF46_004615"
     CDS             complement(<1..932)
                     /gene="tuf"
                     /locus_tag="QBF46_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ6676711.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVY"
     gene            complement(1047..1122)
                     /locus_tag="QBF46_004616"
     tRNA            complement(1047..1122)
                     /locus_tag="QBF46_004616"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1087..1089),aa:Thr,seq:ggt)
     gene            complement(1129..1203)
                     /locus_tag="QBF46_004617"
     tRNA            complement(1129..1203)
                     /locus_tag="QBF46_004617"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Gly,seq:tcc)
     gene            complement(1320..1404)
                     /locus_tag="QBF46_004618"
     tRNA            complement(1320..1404)
                     /locus_tag="QBF46_004618"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1368..1370),aa:Tyr,seq:gta)
     gene            complement(1413..1488)
                     /locus_tag="QBF46_004619"
     tRNA            complement(1413..1488)
                     /locus_tag="QBF46_004619"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1453..1455),aa:Thr,seq:tgt)
     gene            1850..2800
                     /gene="coaA"
                     /locus_tag="QBF46_004620"
                     /gene_synonym="panK"
     CDS             1850..2800
                     /gene="coaA"
                     /locus_tag="QBF46_004620"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDQ6676712.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2829..3794)
                     /gene="birA"
                     /locus_tag="QBF46_004621"
     CDS             complement(2829..3794)
                     /gene="birA"
                     /locus_tag="QBF46_004621"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDQ6676713.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3791..4819)
                     /gene="murB"
                     /locus_tag="QBF46_004622"
     CDS             complement(3791..4819)
                     /gene="murB"
                     /locus_tag="QBF46_004622"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDQ6676714.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAEALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1257 a         1267 c         1130 g         1320 t
ORIGIN      
        1 atgtacactt cagattcgaa cttggtgtgc ggcttgatgg tgcccggctt agccagtacc
       61 tgaccacgtt cgatttcttc acgtttgata ccacgcagca gaacacctac gttctcacca
      121 gcacggcctt cgtccagcag tttgcggaac atttcaacgc cagtacaggt agacttctga
      181 gtctctttga taccaacgat ttcaacttct tcaccaactt tgatgatacc gcgttctaca
      241 cgaccggtaa caacggtacc acgaccggag atggagaata cgtcttcgat cggcagcagg
      301 aacggcttgt caatcgcacg ctctggttcc ggaatgtaag aatccaggaa gccagccagt
      361 tccaggattt tcgcttccca ctctgcgtcg ccttccagcg ctttcagagc agaaccacga
      421 acgatcggag tgtcgtcgcc cgggaagtcg tactgagaca gaagttcacg aacttccatt
      481 tcaaccagtt ccagcagctc ttcgtcatca accatgtcgc atttgttcag gaacacgatg
      541 atgtacggaa cgcctacctg acgacccagc aggatgtgct cacgagtctg cggcatcggg
      601 ccgtcagtcg cagcaactac caggatcgcg ccgtccatct gagcagcacc ggtgatcatg
      661 tttttaacat agtcggcgtg ccccgggcag tctacgtgtg cgtagtgacg ggtcggggtg
      721 tcgtattcaa cgtgagaagt gttgatggtg ataccacgag ctttttcttc cggcgcgtta
      781 tcgatctggt cgaatgcgcg agcagcaccg ccgtaggttt tagccagtac ggtagtgatt
      841 gcagcggtca gcgttgtttt accatggtca acgtggccga tagtaccgac gttaacgtgc
      901 ggttttgtac gttcaaactt ttctttagac atcgattgtc cctctaagac acggataaat
      961 cggtgatatc accacatcaa ccaggcaaca tgcccgactt gttgaatgca ataaacagaa
     1021 ggaaaaaaca gggaggagaa aagaagtggt gctgataggc agattcgaac tgccgacctc
     1081 acccttacca agggtgcgct ctaccaactg agctatatca gcacatcttg gagcgggcag
     1141 cgggaatcga acccgcatca tcagcttgga aggctgaggt aatagccatt atacgatgcc
     1201 cgcatcctgg aactcggcta cctgattttc attctgcact gaatatcgag agaagctctc
     1261 tttattcgag ccggtaagcg aacttatcgt ctcgggctac gccatcgcgt ggccgaaatt
     1321 ggtggtgggg gaaggattcg aaccttcgaa gtctgtgacg gcagatttac agtctgctcc
     1381 ctttggccgc tcgggaaccc caccggactt gatggtgccg actaccggaa tcgaactggt
     1441 gacctactga ttacaagtca gttgctctac ctactgagct aagtcggcat caagtagcgc
     1501 gcattctatg gagacatgcg agttcatgca actaaaaaat tgcataattt gttttattgg
     1561 tcacatttta tgcgacacga tgaagaaaca gccgaacgga gggcagatta gcacactttt
     1621 tcaacatcat tgtgctcaac aatgcgctcc tgctaaacca taattctttt tatcagatgg
     1681 aatatctgtc acattgcttt tcaacgatag cttcctggca gagatttttt cttattattc
     1741 ctccccatct ggtgttaccc tcctgcccat taacccattc aacagaactg tgacgcgcca
     1801 tggcaaatat cgctttgccg atagagctat gaccgccaga aacatgctta tgagtataaa
     1861 agagcaaacg ttaatgacgc cttacctaca gtttgaccgc aaccagtggg cagctctgcg
     1921 tgattccgta cctatgacgt tatcggaaga tgagatcgcc cgtctcaaag gtattaatga
     1981 agatctctcg ttagaagaag ttgccgagat ctatttacct ttgtcacgtt tgctgaactt
     2041 ctatataagc tcgaatctgc gccgtcaggc agttctggaa cagtttcttg gtaccaacgg
     2101 gcaacgcatt ccttacatta tcagtattgc tggcagtgtc gcggtgggga aaagtacaac
     2161 cgcccgtgta ttgcaggcgc tattaagccg ttggccggaa catcgtcgtg ttgaactgat
     2221 cactacagat ggcttccttc accctaatca ggttctgaaa gaacgtggtc tgatgaagaa
     2281 gaaaggcttc ccggaatcgt atgatatgca tcgcctggtg aagtttgttt ccgatctcaa
     2341 atccggcgtg ccaaacgtta cagcccctgt ttactcgcat cttatttatg atgtgatccc
     2401 ggatggagat aaaacggttg ttcagcctga tattttaatt cttgaagggt taaatgtctt
     2461 acagagcggg atggattatc cacacgatcc acatcatgta tttgtttctg attttgtcga
     2521 tttttcgata tatgttgatg caccggaaga cttacttcag acgtggtata tcaaccgttt
     2581 tctgaaattc cgcgaagggg cttttaccga cccggattcc tattttcata actacgcgaa
     2641 attaactaaa gaagaagcga ttaagactgc catgacattg tggaaagaga tcaactggct
     2701 gaacttaaag caaaatattc tacctactcg tgagcgcgcc agtttaatcc tgacgaaaag
     2761 tgctaatcat gcggtagaag aggtcagact acgcaaataa tttgcagggg agcgaatact
     2821 cccctttctt atttttctgc actacgcagg gatatttcac cgcccatcca gggttttatt
     2881 attccatcct gctcaagtaa taaagccccc tgtttgtcta ttccgcgtga aatgccaaat
     2941 atttctttat caccaatgat aagtttcact gggcgattaa taaaattatc cagcttttcc
     3001 cagcgcgaca gataaggtgc caatccttct tgttcgaaga gttccaacgc agcacgtaat
     3061 tcacgtatta gcatggccgc caacgtatta cgatcgagat tgatccccgc ttcctgcagc
     3121 gtgatccacc cctgattaac gacactctct tcaacacggc gcattgccat gttgatcccg
     3181 gctccaatga ctatttgcgc cgcatcgcca gttttgccag tcagctccac aagaatgcct
     3241 gccagcttgc gatcctgcag atagaggtca ttaggccatt tgacacgaac tttatctgct
     3301 cccagcttgc gtaatacttc cgccatcacg ataccgataa ccagacttaa accaatcgcc
     3361 gccgccgggc cttgttccag acgccagaac atcgacaaat ataagtttgc gccaaaaggc
     3421 gaaaaccatt tccgcccccg gcgaccacgg ccagcctgct ggtattctgc aacacaggca
     3481 tcgcccgatt taagctctcc gatacgatca agaaggtact gattcgtgga gtcaataact
     3541 ggcagcacgg ctacactacc gccatccagc tgacccaata tctgttcagc attaagtaac
     3601 tggatgggct caggcaggct gtatccttta cccggaacgg taaagacatc aacgccccag
     3661 tcacgcagtg tctgaatgtg tttattaata gccgcccggc tcattcccag cgtttcaccc
     3721 aactgctcgc cagagtgaaa ttcaccgttc gctaacaggg caatcaattt cagtggcacg
     3781 gtgttatcct tcatgaaatt gtctccactg cgctcacttc acctgatgca ccaataaagc
     3841 ggacttcagg ctcaagccag acattaaatt tttcacccac tttttgtctt acatggtgtg
     3901 ccagttgcac cacatcttcg ctttttgcat tgtcttcatt aatgagaact aacgcctgtt
     3961 gacggtgcac cgcagcccca cccatttgca tcccttttag ctggcactga tcaataagcc
     4021 aacctgctgc cagttttact gaaccatccg cctgggggta atttggtgct gttggaaatt
     4081 gtgacagtaa tgcttcagcc gtttcggcag atacaacagg gtttttgaag aaactaccgg
     4141 cattgccatt cacttttgga tcagggagtt tggtggtgcg catatgacac accgcattaa
     4201 atacttgctg tggcgttact gttgtaggat ccagacgagt taagtcacca tacgttagta
     4261 caggttgcca ctcttttggc agacgcagac ctacggctac aatggcgaag cgatcctggt
     4321 attcatgttt aaaaatactg tcgcgatagc caaaacggca ctctttggca gttaagcgca
     4381 cttgcttgcc tgtcgccagt tcaacacaat caacataagc acaaactcgc tgtaattcta
     4441 cgccataagc accaatattc tggataggtg atgagccgac acaaccagga attaatgcca
     4501 gattttccag accaggcata ccttcctgca aagtgtattt taccaggcga tgccagtttt
     4561 ctccggctcc tacatgtaaa taccacgcat caggttcatc atgaatttcg atacctttga
     4621 tccggttgat gatcaccgtg ccgcgatagt cttccagaaa aagtacatta cttccttcac
     4681 ccagaataag aacgggttgt ccttctgcgg ttgcatgctg ccaggcattg agtaattgtt
     4741 gttcgtcttc ggcacataca atgtgctgag cattatgatc aatgccaaat gtgttccagg
     4801 gttttaagga gtggttcata gctgctttcc tgatgcaaaa acgaggctag tttaccgtat
     4861 ctgtgggggg atggcttgta gatatgacga caggaagagt ttgtagaaac gcaaaaaggc
     4921 catccgtcag gatggtcttc tgcttaattt gatgcctggc agttccctac tctc
//
LOCUS       DAOFQD010000218         4880 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.219, whole genome shotgun sequence.
ACCESSION   DAOFQD010000218 DAOFQD010000000
VERSION     DAOFQD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.219"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            203..2392
                     /locus_tag="QBF46_004623"
     CDS             203..2392
                     /locus_tag="QBF46_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDQ6676715.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALEQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2442..3644)
                     /gene="nupC"
                     /locus_tag="QBF46_004624"
     CDS             complement(2442..3644)
                     /gene="nupC"
                     /locus_tag="QBF46_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDQ6676716.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            3980..>4880
                     /locus_tag="QBF46_004625"
     CDS             3980..>4880
                     /locus_tag="QBF46_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDQ6676717.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGL"
BASE COUNT         1174 a         1130 c         1264 g         1312 t
ORIGIN      
        1 caaccctcgc tgcaaagcgg gggttttttg tctctgcttt ttgccgcttt tgtaatacag
       61 tctacgtccg ggttagtgcc gcctggtgaa agcatcattg gatgaaaaat cggcaacagg
      121 ctggccccct gtttgcttcg cgatgcgaat aaacttatta tttgtgtgcc tgaaaacccc
      181 gatcagtgag agtagtgtac tcatgtttgt ggagcataac ctgataaaaa atatcaagat
      241 attcacacta gcgtttacgc tcaccgtggt acttattcag ctatcccgtt ttatttcgcc
      301 acttgccatt atccattcca gttatatctt tctggcgtgg atgccactgt gcgtaatgct
      361 gtcaatcttg tttatctttg gctggcgcgg tgtcgttccc gttttatgcg ggatgttttg
      421 caccaatctg tggaactttc atctctcttt tttacagact gcggtcatgc tcggtagcca
      481 gacgtttgtc gtgttgtgtg cctgcgcaat attacgctgg cagctgggga cgcgttggcg
      541 ttatggattg accagccgat atgtctggca acgtctgttc tggcttggtt tggtgacgcc
      601 gatcggcatc aaatgcagca tgtatcttgt ggggagcttc tttgatttcc cgctaaagat
      661 atccaccttt ttcggcgatg cggatgccat tttcacggtc gttgatttgc taagcctttt
      721 caccgctgtg ctgatttaca acatgctttt ctactatctc actcgcatga ttgtaagtcc
      781 ccactttgcg cagatattgt ggcgcaggga tatcgctccg tcgttgggca aagagaaacg
      841 cgcatttacc ttaagctggc tggcagcgtt aagcgtgctg ctacttctgt tgtgcacacc
      901 ttatgaaaac gactttattg ccggttacct ggtacccgtt ttcttcatca tctttaccct
      961 cggggtcggt aagcttcgct atccgttttt aaatctcacc tgggctgttt caacgctttg
     1021 ccttctgaat tacaaccaga actttttgca aggggtggaa accgaatatt cgctggcatt
     1081 tattcttgcg gtgctgattt cctttagcgt ttgcctgctc tatatggtgc gcatttatca
     1141 tcgcagtgaa tggcttaatc gccgctggca tttgcaggcg ctgacagatc cgttaacgct
     1201 cctacccaac tttcgtgcgt tggaacaagc gccggagcaa gaggcgggca agagtttttg
     1261 ctgcctgcgc attgataatc ttgagtttat gagtcgtcat tacggcttaa tgatgcgcgt
     1321 tcactgtatc cgctcaattt gccgtacgct gctgccgttg atgcaggaaa acgaaaagtt
     1381 gtatcaattg ccgggtagtg aactgctgtt agtgctgagc gggccggaaa cggaagggcg
     1441 actccagcat atggttaaca tcctgaatag tcggcaaatt cactggaaca ataccgggct
     1501 ggatatgggc tatggtgctg cctgggggcg ttttgatgga aatcaggaaa ccctgcaacc
     1561 cttgttgggg cagttaagct ggctggcgga gcaatcctgc gcacatcatc atgtgctggc
     1621 gctggatagc agagaggaga tggtttccgg gcagaccact aaacaggtgc tattgctgaa
     1681 taccattcgc acggcgttag agcagggtga tttgctgctc tacgcccagc caattcgcaa
     1741 caaagagggt gaaggttatg atgagatcct cgcgcgactg aaatatgacg gcggcattat
     1801 gaccccggat aagtttctgc cccttattgc tcagtttaac cttagcgcgc gttttgattt
     1861 gcaagtgctg gaatccttgt tgaagtggct ggcaacacac ccttgcgaca aaaaaggacc
     1921 gcgcttttca gtcaatttaa tgccgctcac gctgctgcaa aagaatattg ccgggcggat
     1981 tattcgtctg tttaagcgtt atcacatctc cccgcaggcg gtcattcttg agatcaccga
     2041 ggagcaggcg ttttctaacg cagaaagcag catgtacaac atcgagcagc tgcataagtt
     2101 tggtttccgg attgcgattg atgactttgg caccggatat gccaactacg aacggttaaa
     2161 gcgtttgcag gctgatatca tcaaaattga tggcgtcttt gtgaaagata ttgtcacgaa
     2221 cacgctggat gcgatgattg tgagatcaat taccgatctg gcgaaagcga agtcattgag
     2281 tgtggtcgcg gagtttgtcg agacgcaaca gcagcaggcg ctattgcata agctcggggt
     2341 gcaatatctg caagggtatt tgattggtcg cccgcagcca ttagctgatt aacaggcgta
     2401 aaaaaaccgg ggaattatcc ccggcttttt tatgtatggt cttacagcac cagtgctgcg
     2461 attgacgcag acagcacact caccagggta gagccgtaaa ccagcttcag accgaagcga
     2521 gaaaccacgt taccttgctc ttcattcagg cctttaactg cacctgcgat aatcccgatt
     2581 gaagagaagt tagcgaagga aaccaggaac acagagatga tgccttcagc acgcggagag
     2641 agcgtggaag caattttctg cagatccatc atcgcaacga actcgttgga aaccagtttg
     2701 gtcgccatga tactgcccac ttgcagtgct tcactggaag gaacacccat cacccatgca
     2761 atcggataga agatgtagcc caggatgccc tggaaggaga tgctgtagcc aaaccagcca
     2821 gtaacggtgg caaacagtgc gttcagcgcg gcgatcaggg cgataaagcc aatcagcatc
     2881 gcggcaacga taatggcaac tttgaaacct gccagaatgt attcacccag catttcgaag
     2941 aagctctgac cttcgtgcag gttggacatc tggatgtttt cttcactggc atcaacacgg
     3001 taaggattga tcagcgacag cacgataaag gtgctgaaca tgttcagtac cagcgcagca
     3061 acgacgtatt tcggttccag catggtcatg tatgcaccaa cgatggacat cgacacggtg
     3121 gacattgccg tggcagccat ggtgtacata cgattacggg agattttgcc gaggatatct
     3181 ttataggcaa taaagttttc agactgaccc agaatcaggg agctgacggc gttaaaggat
     3241 tccagtttgc ccatgccgtt gactttggag agcaggaaac caattgcgcg gatgatcacc
     3301 ggcaacacgc gaatgtgctg gagaataccg atcagtgcag agataaagac gattgggcac
     3361 agcactttca ggaagaagaa tgccaggcct tgatcattca tgctaccaaa gacgaagtta
     3421 gtcccttcgt tggcaaatcc gagcagtttt tcgaacattt cggagaagcc tttcacgaag
     3481 cctaaaccaa cgtcggagtt caggaagaac cacgccagta acacttcgat aacaagcagt
     3541 tgaataacat aacggatacg aatttttttg cggtcgctgc ttaccagcag tgcgagaatc
     3601 gcaacaacgg caagtgccag tacaaaatga aggacgcggt ccatatttgc tccaaatatg
     3661 aggcaggtta aatttccgtg cacattctat gtaacatggt taaggaaaac gagatctgac
     3721 acacactata atgacgtcct gtgacaggct tcaaaaatag tgatctgtca tacatttctg
     3781 ctatgttgtg tatggaagct gaaagttatg taaatgtgct aacattaaac ataaccataa
     3841 cattcaagtc atcaatcttt tcgtagtcat aatcctggtc tatcagagaa atcaccacaa
     3901 tccatttaaa tgaatttgat aatcattctc gtttggcata acatgaaaca tagcaaaggc
     3961 tatgttttag aggcacaaga tgacgaacta tcgcgttgag agtagcagcg gacgggcggc
     4021 gcgcaagatg aggctcgcat taatgggacc tgcgttcatt gcggcgattg gttatatcga
     4081 tcccggtaac tttgcgacca atattcaggc gggtgctagc ttcggctatc agctactgtg
     4141 ggttgtcgtt tgggccaacc tgatggcgat gctgattcag atcctctctg ccaaactagg
     4201 gattgccacc ggtaaaaatc tggcggagca gattcgcgat cactatccgc gtcccgtagt
     4261 gtggttctat tgggttcagg cagaaattat tgcgatggca accgacctgg cggaatttat
     4321 tggtgcggcg atcggtttta aactcattct tggtgtttcg ttgttgcagg gcgcggtgct
     4381 gacggggatc gcgactttcc tgattttaat gctgcaacgt cgcgggcaaa aaccgctgga
     4441 gaaagtgatt ggcgggttac tgttgtttgt tgccgcggct tacattgtcg agttgatttt
     4501 ctcccagcct aacctggcgc agctgggtaa aggaatggtg atcccgagtt tacctacttc
     4561 ggaagcggtc ttcctggcag caggcgtgtt aggggcgacg attatgccgc atgtgattta
     4621 tttgcactcc tcgctcactc agcatttaca tggcggttcg cgtcaacaac gttattccgc
     4681 caccaaatgg gatgtggcta tcgccatgac tattgccggt tttgtcaatc tggcgatgat
     4741 ggctacagct gcggcggcgt tccacttttc cggtcatact ggtgttgccg atcttgatga
     4801 ggcttatctg acgctgcaac cgctgttaag ccacgctgcg gcaacggtct ttggattaag
     4861 cctggttgct gcggggctgt
//
LOCUS       DAOFQD010000219         4769 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.220, whole genome shotgun sequence.
ACCESSION   DAOFQD010000219 DAOFQD010000000
VERSION     DAOFQD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.220"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(13..387)
                     /locus_tag="QBF46_004626"
                     /pseudo
     CDS             complement(13..387)
                     /locus_tag="QBF46_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(689..1390)
                     /locus_tag="QBF46_004627"
     CDS             complement(689..1390)
                     /locus_tag="QBF46_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676718.1"
                     /translation="MKIALVLRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILENPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKVHIWSAFWQDPEGWIARCVTTECWGEQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLISSFFERESE"
     gene            complement(1387..2064)
                     /locus_tag="QBF46_004628"
     CDS             complement(1387..2064)
                     /locus_tag="QBF46_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDQ6676719.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISFGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILAWALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQHYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            complement(2061..2687)
                     /locus_tag="QBF46_004629"
     CDS             complement(2061..2687)
                     /locus_tag="QBF46_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDQ6676720.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGTEP
                     KNTNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSAREQIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            complement(2585..3247)
                     /locus_tag="QBF46_004630"
     CDS             complement(2585..3247)
                     /locus_tag="QBF46_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDQ6676721.1"
                     /translation="MLAWRLNLQLRMNIPPRATRTVFCVGSGPSLTREDCAAIEKTGC
                     SIIAVNNSWQMFDDIYALYAGDLSWWKQYGSTIPGGRFRKVTANLAAAKSFSLEYRRY
                     CGPAEGVNSGAQAISLAAESGAEVVVLVGYDCSLQNGLHWHGAHPQALRNPTQVSISK
                     WQQQFLDTRKKHADLHILNASRSSAIQCFPRINLEAVIALLSSAVAQAPQTLLRRAEC
                     RL"
     gene            complement(3189..3344)
                     /locus_tag="QBF46_004631"
     CDS             complement(3189..3344)
                     /locus_tag="QBF46_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDQ6676722.1"
                     /translation="MSFKANYESLASIMPRNEQEADAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            complement(3411..3662)
                     /locus_tag="QBF46_004632"
                     /pseudo
     CDS             complement(3411..3662)
                     /locus_tag="QBF46_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
     gene            complement(3665..4321)
                     /locus_tag="QBF46_004633"
                     /pseudo
     CDS             complement(3665..4321)
                     /locus_tag="QBF46_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4564..4668)
                     /locus_tag="QBF46_004634"
                     /pseudo
     CDS             complement(4564..4668)
                     /locus_tag="QBF46_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1213 a         1254 c         1085 g         1217 t
ORIGIN      
        1 gagtcattac ccggcattat cagcacgctg gttggcgtca tgcgtcgcat cgtcgatcgc
       61 gtcctgttgc tggcgtttca aatgttcaat ttgcagctga agctcttcaa tttctttacc
      121 cttcagaccg agatccaact gcatattttc agctgcatct accagggagt tatggctatc
      181 agcttctgcg ttataaacat caataagctg tgcgtgaagc atctccgctg actgaaccgc
      241 attatcaaaa aaacgtgctg tgaggtcatc acaactaacg cggcgttgcg cggcccggat
      301 gttctggata atggccggga taccggcatt caggacatca gggatagata cattttcgat
      361 tgattggttt tgtgctgaag tgctcatttc aaagttccgt attagcttgt gcttcggtca
      421 tttttcctaa gtatgaaggt ggaaggacta cgcaatttgt atccagtccc tcacctatgg
      481 cagcctgtaa aattctggct aaggtgagtc tcttgttgcg atacctggtg atgacatgcc
      541 tgataccgcc ggtcagcgta acaaaggcga tcagccagta gtgatatttc cgtcggaatg
      601 gccacatagt gcaccttata gattgctcta ataaaaaacg tgatgagtgt acatcacgtt
      661 ttaaaaatat ggaattatta gagcaatatt attctgattc tcgctcaaaa aatgagctga
      721 taaggggaag ccaatcctct gacacttcgc gaggtcgcgg tttgccgtgg aaaaagatta
      781 ttcggcagtc ttttggtaat gccccattcc ccctggagta acgcgcgctc gcatatttcg
      841 aaccaggttc cacaacatcg gccttgtaac ttacaaacca tcccggatac agatcctgaa
      901 atgctggtgt atcatcaccc ataaccttcc gcaaaaaccc ctgctcaccc cagcattcag
      961 tagtaacaca acgagcaatc caaccttccg gatcttgcca gaatgcactc cagatatgca
     1021 cttttacact atttggtatc cacagggcac cgctgccacg atattgtgga tggtaaaaat
     1081 ccctaagcat ggtgaagctg gttggtggat tctctaggat tgggcgtata tcaccggcaa
     1141 taaccgtgtc caaatccaga tagaacagat catcggttat atccggtcgg aacaactcga
     1201 ttttcgccca ccagccacgg cacttttgcc actggttgat caatgggaca actttgacgc
     1261 caggtacatg taaacgcttc aggtctgtca ggcaaataat ttcatagcct tttggcagtt
     1321 gattaaccag ccactgcaca tcggaagcgt tatagtcacc accagagcga agaactaaag
     1381 caatcttcat gctgcaccat cacctttcac tttcatcaat gtcaggtttc cgcaaaatac
     1441 ggcacctgtg tcgatataat gctgattcca gaatgtcttc gggcttttca ccggagtgtg
     1501 accaaagata aaacgatctg cgcccgaaat ttcgccacca atatcatcca tcgaatcact
     1561 gatacgctcg cgcgcccaga caacgttgaa aagcggcacc tccttaccga attggtattc
     1621 attatccgga tagtcggcat gggctataac gatagtttct tgcccggtgt tcaactcaat
     1681 gatatagggc agacgcttta ccagctccac cagcgcccag gctaatattt cctgatcagt
     1741 gtccagcatg aagaaccatt gtccgccatt cattagccag ttattcacgt tgccatctgg
     1801 acttaacgca tcaatcatca gccgctcatg gttccccatc actgccctga accagggcat
     1861 ctgcaatagt tccagacatt cgacattttc agtaccgcga tcgataaggt cgccgaacga
     1921 tatcagtaaa tcctgcgccg ggtcaaaatc cacacgatgg agttcggaca tcagtctggt
     1981 gtagcaacca tgcagatcac caacaaccca gacattcctg tatttggtac cgtcgatacg
     2041 gtgataaatt gtgggtgcca tcatgtattc ttcagccatt ctttaagagt catctgcgga
     2101 atacctccca ttttcccgca tgaaacaacg tcaatctgtt cacgcgcaga ctggaataac
     2161 aaaggcaggt gacttagatt ttttggcgtg ccgccggagt gaacgcgtgg ttcttgtgta
     2221 gcgtcaacgc ccaccagggc gacatgtttg aatccgatat ggaaagccag gttcagagca
     2281 ccatatgcac tattgccgct ggcaatttca ttctcatctt cgcaaaggcc gaaatgtgcg
     2341 gaccagcgcc acgcccacca ctcgggagaa ttcgtatttt ttggctccgt gccgcgctca
     2401 gccacacgac ggaagcacag aacgccatct ctgacttcac gttctttaac atcgggtagt
     2461 gccatgcaat aacaaacacc acggcgacgg cggccacgac caacgcgccg catattgtct
     2521 ggcgatggat caagggtgaa aaaataagaa gcgcggttaa gccagtcgat ggccccattg
     2581 accgctataa tcggcactcc gcgcggcgca acaaagtttg cggcgcttgg gccactgccg
     2641 acgataataa cgcgatcact gcctctaaat ttattcttgg gaaacattga attgcactgc
     2701 tcctacttgc attcaaaata tgtaaatctg cgtgtttttt gcgggtatcc aggaactgct
     2761 gttgccattt tgaaatagac acctgcgttg gattccgtag ggcttgaggg tgcgcgccat
     2821 gccaatgaag gccgttttgc agagaacagt catagccgac taataccact acttcagccc
     2881 ctgattcagc agccagactg atagcctgcg cgccgctatt taccccttcc gccggtccac
     2941 aatatcgcct gtactccaac gaaaatgatt tcgccgccgc caggttggct gtcactttgc
     3001 ggaatctccc tcccggtatg gtggatccgt attgcttcca ccatgacaaa tcaccggcgt
     3061 ataaggcata aatgtcatcg aacatctgcc aggaattgtt aaccgcgatg attgaacagc
     3121 cagttttttc tatagcagca cagtcctcac gagtgagtga cggaccgcta ccgacacaaa
     3181 aaacagtcct agtcgccctg ggtggtatgt tcattctcag ctgcaaattc agcctccagg
     3241 cgagcattca tttcagcgat taccgggtcc actacagcat ctgcttcctg ttcattacgc
     3301 ggcatgatcg atgccagcga ttcataatta gccttgaatg acacgattat tctcccgatg
     3361 ttaatgtgcg ctatatcaaa gagcacatat gcactaatta atttattatt ttaagcagca
     3421 tacaaccact tgtcgccgtt caatacatgc tcaatagcct cacccttttt aaggcttatg
     3481 tattccagga tggcggttat cgcttgttct gcaccatacg caagaacgac gtagtaacct
     3541 tcctctctaa gcctgcgcat ccaggcgatc tgctctttcg tcggggcttt accatttggt
     3601 tctttaagct caattcgcat gccgtgataa ataccgcatg ctttatcgag actcatgtcc
     3661 ggacatccag atcagcgatt tcgtcatgta actcgagata gcgtcgggca agggacttta
     3721 atgaaatgcg ataaacgttg gttacattgc ggtattcact ggcatcaggt ctccaggaac
     3781 ccagggtcct gatgagctgc atgcgcgtca tatttctgag ctgttcacgt aattcatccg
     3841 gggcagagat aatatttgaa tggataatct ggagagcgac tctgcgggct gatattgctg
     3901 tttttcggca agtttttaat acccgcaggg actcaatcat gccatcacgc gttttgggag
     3961 taactgtcct gatgcctgag aatgctgcat gagcggcaca ttcagcatca attgtgtcac
     4021 ttttaccccg tttgcgtcgc tccatccggt ctggagcagt cacctcaaga acttctaacc
     4081 cggcgttctg aaaataacga agcaaaccgg aaccataggt acctgtgcac tcaacaccaa
     4141 ttcgctttaa tgtcccaaac gaggtcatcc atgccagcat ctgccggtaa ccttgtcgtg
     4201 ttgtggagaa atactgagtc ccaaggactt tattgttctg atctacgaca gcggcaacgt
     4261 gcaaatcttt atgtgtatcc acgccaccca caacggcagc tttggtaact ttatcaacca
     4321 tgacagagct cctgtgagga aacagagttg aaactccaga cagataaccc ggacaggaca
     4381 gtaacgagac aagccgtcag gcccttcttg agtcacgcgc atcggtgagg agatgcctcg
     4441 catgaaggcg cttccggcaa ccgacgggtc cagggcagga cacaaaaggt cgatcgctgt
     4501 gtgagtcagg atgcgggaag gtcttcactg catcagtaat cacaccaatc tggttaaacg
     4561 gcaaactaag tggtggtaaa gactgacatc ggcattatta ctatcgctgt ggaacatcac
     4621 cccgacgggc ttaccacggg tttcccatgc catttccagt gctttcatgg taagcctgct
     4681 gtccggcgag aacgacatgg cccagcccac tggttttctt gcgaacaggt cgagaacaac
     4741 ggcgaggtac gcccagcgct tacccgtcc
//
LOCUS       DAOFQD010000220         4708 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.221, whole genome shotgun sequence.
ACCESSION   DAOFQD010000220 DAOFQD010000000
VERSION     DAOFQD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.221"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(866..1891)
                     /locus_tag="QBF46_004635"
     CDS             complement(866..1891)
                     /locus_tag="QBF46_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HDQ6676723.1"
                     /translation="MIKYCCASCFGDNGLKKNIIPSLSPDFGDCSYCGTKGIEIIEPH
                     ALFDHFSSLINIYENHPDGKSLVAHLKEDWKLFSHPLLDEAHAKELLGDILDDGNVVR
                     KTFVLSDSYKSESLALWDHLCQELMYENRYFLNKSLDTDRLGELLSFLALDDFSKIWY
                     RARIYADDTAFTIDKMGAPPKRLVSHGRANPTGIPYLYLGSKPDTALSEIRPHTGDRA
                     CVAEFTLRDNLTLIDLRNPRETVSPFLLTDPGEMGKMLADIPFLERLGYELTRPVLPK
                     SAALDYIPSQYICEFIKKTGFHGVLYSSSVSEGINLALFDPLNATGGNVSIYDIDKVE
                     VSVSKQD"
     gene            complement(1893..2825)
                     /locus_tag="QBF46_004636"
     CDS             complement(1893..2825)
                     /locus_tag="QBF46_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDQ6676724.1"
                     /translation="MYYPYFRGKQFELLAIRDTANVMAEAGFTPIIEPVREALNGLER
                     TLKTIKENDGRAIVIINPACGDHSTDGQSIAQLLKNEFSKYEKISVGILLDKSMTIAK
                     VDNLLHSDLVHEVTFIHAGFDEPKALHERFGDGYKHVFISNYCSKAYRKHFSGSERVL
                     IKDGFEKRRNADHPDSEFFSDLHLNYEEEGMNGFGDFLIVGDEFSESGGPAYAVAIHL
                     TYINTKHEDEMYIYHFVSTTRDTPTDPAGKFAQSLDKLMVALNSGKSMLLETSAIKEF
                     RRLHNPSHFPGLGTVKKLSMIHHIETLADFMDGK"
     gene            complement(2832..3734)
                     /locus_tag="QBF46_004637"
     CDS             complement(2832..3734)
                     /locus_tag="QBF46_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HDQ6676725.1"
                     /translation="MMRSNELNSFQLTALSRLFSASVFSEIAKRGQSPIFARSLRETE
                     LFDHADTLGITVGEAFNEAFALLRQTGLRNEYVYRSALTHNLLLGKHSLRTACMLNEF
                     RIGSCKADLIILNGTGTVYEIKSERDSLSRLQNQITNYRKAFGKIYVIAGSEHLDDVL
                     KTTESSIGVLSLTRWNRISTIREAEEVLDFICPVTIFESLRINEAKIIASELGIKIPD
                     LPNTQMHGFLKEEFSGLPPVSVYNLMLKTLKESRSLLSLRELILNLPKSLQPAALSIQ
                     LRSKDHERLINTMQLPLNEALLWE"
     gene            4261..4674
                     /locus_tag="QBF46_004638"
     CDS             4261..4674
                     /locus_tag="QBF46_004638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676726.1"
                     /translation="MYSLVIAEIFIYKNPTISDTKAYENIYITFNPYSGTDFRSHASL
                     SDAQQYVDNENKNESKRSEAQKSDVITDTLKLKYDNSIEDTVSIDKDTSLFLEKYNKT
                     HNTHSLSKTLFTVTEKHIMNDELDSTNVLVKRNIP"
BASE COUNT         1559 a          928 c          824 g         1397 t
ORIGIN      
        1 gggcaagctg ggttccaagg ctacgtgata tttgggttca caggccaaaa catttacgtg
       61 ctagaaagct cacaatgtaa taacgcaact tatatactta acaataattg gcagcagtta
      121 tcccagctaa ctaaagccga aatactagac ggaaacttgc accatgcccg tgtcatccac
      181 cgtgtaaact gggcagcaga aatacttaaa tacttaccat aaagcgccgg acgcgcggga
      241 atgaaccccc gcgcactttc aaacataccg caatagaatc cataaagccc cgttggtggt
      301 aagacaacaa gcggagcctc tggcgactac atcacagtta tgtcagttag tgaggcttat
      361 caggatgacc atgtacccaa ggggagcgag gccgaaccgc tggcagagct tgcatatggt
      421 ctgcaagtac cgtgaccacc ataaacctgt ttatcccctg cggcgtctcg cgacagcggc
      481 agaacggggg catcgggcta aaatgcgctt cactaaaagt ttttgggatt gtttagagac
      541 agtggctacg tcatagcttt caagttgagt cagtacgtgt ttgacgcttg gaactacacg
      601 ccgctatgag ttaacatccc aattaccact atcaaccaca gcggcttttt caagggacat
      661 aacagagcat ttcttacagg caaaagagac agattttatt gtctaactac ctgtattgta
      721 aggaatttag tgacttggtg cgaaggccgg actcgaacat cgctactatc cttatgatct
      781 taagggatta aaacttacca aattgggcat gataccatca ttgataccat cagtcccgac
      841 tggtgataat cgaacaaaga gtatattaat cttgtttgct tacactaact tcgactttat
      901 ctatatcata aatacttaca ttgcctccag tcgcatttaa cggatcaaaa agagcaagat
      961 tgattccttc acttacagaa ctactataaa gtactccatg aaagccagtt ttcttaataa
     1021 attcgcatat atattgactt ggaatataat caagggctgc acttttaggt aatacaggtc
     1081 ttgttagttc ataaccaagt cgttcaagaa aaggtatatc tgcaagcatt ttacccattt
     1141 cacctggatc ggttaataaa aacggtgaaa cggtttcacg agggtttctt aggtctatta
     1201 gagtcaaatt atctcttaga gtaaactctg cgacacaagc tctgtcacct gtatgaggtc
     1261 gaatttcaga caaagctgta tctggtttag atccaagata taaatacggt atacctgtag
     1321 gatttgctct cccatgacta actaatctct ttgggggagc ccccattttg tctatcgtaa
     1381 aagcagtatc gtctgcataa atacgggctc gataccaaat tttagaaaaa tcatctaaag
     1441 caaggaaact taataactcg ccaagcctat ctgtatcaag tgatttattc aaaaaatatc
     1501 tattttcata catgagttcc tgacaaagat gatcccataa tgcgagagat tcactcttat
     1561 aactatcaga aagtacgaag gtttttctaa caacatttcc atcatcaagt atatcaccta
     1621 ataactcttt ggcatgagcc tcatctaaaa gaggatgtga aaatagcttc caatcctctt
     1681 tcaaatgtgc aactagactc ttaccatccg gatgattttc gtagatgttt atcaatgaac
     1741 taaaatgatc aaataaagca tgaggctcaa taatctcaat acctttggta ccacaatacg
     1801 aacaatcgcc aaaatctggg gaaagtgaag gtatgatatt tttctttaaa ccattatccc
     1861 caaaacagct cgcacagcag tacttgatca tattatttac catccatgaa atcggctagg
     1921 gtttctatat ggtgaatcat agacaatttt ttaactgtcc ctaaaccagg gaagtgactc
     1981 ggattatgta atcgcctaaa ctctttgata gccgacgttt caagcaacat ggatttgcct
     2041 gaatttaaag caaccattaa tttatcaagg gactgtgcga acttacctgc tgggtctgtc
     2101 ggtgtatcac gtgttgtcga aacaaaatga tagatgtaca tttcatcttc atgcttcgta
     2161 ttaatgtaag taagatgaat agcaaccgca tacgcagggc caccggactc actaaattca
     2221 tcaccaacaa ttaagaaatc accaaaacca ttcataccct cttcttcata attaagatga
     2281 agatctgaaa agaactcaga atcgggatgg tcagcatttc gtcttttttc aaagccatct
     2341 ttaattaaaa ctctttctga acccgaaaaa tgttttcgat aagctttaga acaatagtta
     2401 gaaataaata catgtttgta tccatcccca aatctttcat gtaaagcttt tggctcatca
     2461 aatccggcat gaataaaggt gacttcatga acgagatctg aatgaaggag attatcaacc
     2521 ttagcaatag tcatactctt atcaagaagt attcctacag aaattttttc atacttacta
     2581 aattcatttt ttaataattg agctatactt tgtccatcgg tagagtgatc accacatgcc
     2641 ggattaatga tgacaatagc cctaccatca ttttctttta ttgttttcag tgttcgctca
     2701 agaccattga gagcctctct aacaggctct ataattggag taaaacctgc ctcagccatt
     2761 acattagcag tgtctctaat agccaacagt tcaaattgtt tcccacgaaa ataaggataa
     2821 tacatagttt gttactccca taacaatgcc tcattaagag gaagctgcat agtattaatt
     2881 aatcgctcat gatccttaga gcgaagctga atagaaagtg ccgctggttg taaagacttt
     2941 ggtaagttga gaatcaactc tcttaaagaa agtaaacttc tgctttcctt taaagttttt
     3001 agcattaaat tatatacaga aacaggtggt aggccactaa actcttcttt taagaaacca
     3061 tgcatttggg tattcggtaa gtcggggatt tttataccta actcggaagc aataattttc
     3121 gcctcattaa ttcttaatga ttcaaagatt gttacagggc atataaagtc taaaacttct
     3181 tcagcttctc ttatcgtaga tattcgattc caacgggtta aactcaatac cccaatggaa
     3241 gattcagttg ttttcagcac atcatctaaa tgttctgaac ctgctatcac atagattttc
     3301 ccaaatgctt tacggtagtt cgttatttgg ttttgtagac ggcttaatga atccctttcg
     3361 gattttattt cgtaaaccgt tccagttccg ttcagtatga tgagatcagc tttgcaagag
     3421 ccgattctga attcattaag catacaggca gttcttaatg aatgcttccc aagcaaaaga
     3481 ttgtgtgtga gcgcagaacg ataaacatac tcattccgca agcctgtttg tctaagtaag
     3541 gcaaatgcct cattgaaggc ttcaccaacg gtaatcccaa gtgtatctgc atggtcaaaa
     3601 agctcggtct ctcggagaga acgagcaaat ataggagact gccctctttt cgctatctcg
     3661 ctgaagacgg aagcggaaaa aagtctactt aaagcagtca attgaaaaga gttaagttcg
     3721 ttacttctca tcatggtgaa tcacatagtc catcgttcag gatcttgttt ggttactgtc
     3781 aaaatcctta gcacaaccca cataaaagtc gttcatcagt gtaggaaagc tatgaaaaaa
     3841 atcttcacct taggttacca aaccagcagt gtcctgcata gaaaagtcga tagcctaact
     3901 acatgcaacc aattctagta gcctgaaata attttgtttg tgagatttaa cacaatctat
     3961 tgatcataaa agaaaaaaaa cattgaaaga ggtgatttta tgaccacata cggtaatgtt
     4021 tactataaca tagggtaaaa cacgaatttt ggtggcagac atagtatctt ttatcaaaag
     4081 aatatatgac tataatggat aaaaaatttg ttttatattc ataatagtta tatgaatctc
     4141 aactatggga tactatcccc cattggtcaa catgaagttt tatcatttca ctgaagaaaa
     4201 aaatgaccgc tacaaactat tgtggtatat aaaaaaccaa tcattattga ggatagaatt
     4261 atgtattcat tagttatagc tgaaattttc atttataaaa atccaacgat ttctgataca
     4321 aaagcatatg aaaatattta tatcactttt aacccttata gtggtactga tttccgatca
     4381 catgcatctc tcagtgatgc tcagcaatat gttgataatg aaaataagaa cgaatcaaaa
     4441 agaagtgaag ctcaaaaaag cgatgtaatc accgatactt taaaattgaa atatgacaat
     4501 tccatagaag atacagtcag tatagataaa gacacatcac tgtttttaga aaaatataat
     4561 aaaactcata atacacattc cctttctaaa acattattta ctgttacaga aaagcatatt
     4621 atgaatgatg aattagacag cactaatgtt ttagtgaaga gaaatatacc ttaaaatatc
     4681 attgatatat atgcataata tatctttt
//
LOCUS       DAOFQD010000221         4565 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.222, whole genome shotgun sequence.
ACCESSION   DAOFQD010000221 DAOFQD010000000
VERSION     DAOFQD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.222"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(1104..1988)
                     /locus_tag="QBF46_004639"
     CDS             complement(1104..1988)
                     /locus_tag="QBF46_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HDQ6676727.1"
                     /translation="MSKLSSEMKALAKKAGGSFKTVNDRIHITKRFSEHLRALNIQTQ
                     RVEQIKVRHIECYIEERLEQDIGLRTLQNEMAALRSVLRQAGRRQVVEHPRLTNKALG
                     VSGASRNGTRRAITPEHYQQVMEKARAEDEGLAAALEIARLMGLRSQEAVQSSQSLKT
                     WLKAIERGETRLKVVFGTKGGRPRYTTLLDAGAVRKAVENALPIARQRNGRLIDKPDL
                     KTALEHWHKQVIKAGLKGEFSPHSLRYAWAQDAIRHYLAQGFSEKESLALTATDLGHG
                     DGRGRWVKQVYGYRWKEE"
     gene            complement(2840..3092)
                     /locus_tag="QBF46_004640"
                     /pseudo
     CDS             complement(2840..3092)
                     /locus_tag="QBF46_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3089..3550)
                     /locus_tag="QBF46_004641"
     CDS             complement(3089..3550)
                     /locus_tag="QBF46_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676728.1"
                     /translation="MATTLTSECNLLAVPFSAATDFTDLAEYCDRVATTLIECRDPAL
                     KTALCGRLNTCLALLRPTLNEPIPPHLIDGFITNDLPDVSPGFEPDAGSLCDYCLALT
                     QILNGHALLPETETTLTGLLCELVWYFAADLNAPRWIRTADGIQVIDGRPA"
     gene            4070..>4565
                     /locus_tag="QBF46_004642"
     CDS             4070..>4565
                     /locus_tag="QBF46_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDQ6676729.1"
                     /translation="MSGKPAARQGDATMKGGPIVQGSAGVRIGAPTGVACSVCPGGFT
                     SGNPVNPLLGAKVQPGETDFALPGPLPFVLSRTYSSYRTKTPAPVGIFGPGWKAPFDI
                     RLQIRDNELILNDNGGRSIHFEHLFPGEDGFSRSELFWLVRGGVAKLNESHRLAPLWQ
                     ALPEE"
BASE COUNT         1089 a         1236 c         1137 g         1103 t
ORIGIN      
        1 cagaagatga aaagctatca atacgtgata ttttcactga aatgccaatt aatactccag
       61 aatatctcct gaccctatac tctgggacct gtaagaactg catcagatat tcagagacca
      121 gacagatata agcctgaaat tattttgctt ccaataattt caggagaaac ctatcaatgc
      181 atctgttttc aacaggcagc caccttccgt gattaacatg ttaatcacgg aaggtgaaaa
      241 gctatcaatt atcgatattt ccacggaatt accacttaat acttccaaaa aagtgcgcaa
      301 taaaataccg ggcaatcttt cctgatacac attatcactg caccagctat acagagacaa
      361 aataatctgc accagcccct gtaaatcggt tatctgcgcc agcgtcccgc cgctctacct
      421 gttcaggact gcgccagttc ctggcccttt ggttttcggc cctttcaggc caggcgctgt
      481 tacccttatc gcccccttaa cctttatgac cctttccctt tctggcgcaa ccagtttatt
      541 cggaaatgta ccatgacagt ctgcaccaga ttgtattttc accacaggat aacggtcaca
      601 gagtgcaaaa aataacatga agcaccgaag attgttaatc tatatgtagc aggttatggt
      661 gtcaaaaact gtcgcagcat tttttataaa atcatgagac attctgtgtt catacaatat
      721 atgccacagg atgcaggaaa acacagagag aattattcag atataatcct gcgccagaac
      781 taatgtgatg ccccggtgaa tttacatttt aaaattttcc cagccctgta atatcggtaa
      841 aaacgattaa aacacctctt attaatcggg gaaatttcga gtgattacag gggtaatcac
      901 cataatatcc cagtaatcct gcactgatga agcaggaaat tctgagttat tgatatttat
      961 tcaccatgaa gtaacaatat tctcattata ttgtcacgca gcaacgacag gtatccggtg
     1021 ttttatttca tccggttcct gcgccagact gatcgctgtc gttacacagt aaccgcatta
     1081 ttttgcgcca ttcatgaaat ccgtcattcc tccttccatc ggtatccata cacctgtttc
     1141 acccagcgtc cgcgaccgtc gccgtggccc agatccgtcg ccgtcagcgc cagtgactcc
     1201 ttttcgctga atccctgcgc cagataatgg cggatcgcgt cctgcgccca cgcataacgc
     1261 agcgagtgcg gggaaaattc ccccttcagt cccgccttaa tgacctgttt gtgccagtgt
     1321 tccagcgccg ttttgagatc aggcttgtcg atcagtctgc cattacgctg tctggcaatc
     1381 ggtaaggcgt tttccaccgc tttcctgact gcaccagcgt ccaggagcgt cgtgtaacgt
     1441 ggacgcccgc ctttggtgcc aaataccact ttcagccggg tttcaccgcg ctcaatggct
     1501 ttcagccacg ttttcagcga ctgtgaactc tgtaccgcct cctgcgaacg cagtcccatc
     1561 agccgcgcga tctccagcgc cgccgccagt ccctcgtcct cagcgcgggc tttctccatc
     1621 acctgctggt aatgttccgg cgtgatagcg cggcgcgtgc cgttgcgtga agcgcccgac
     1681 acccccagcg ccttattggt caggcgcgga tgctccacca cctgcctgcg tccagcctgt
     1741 ctcagcacgg atcgcagcgc tgccatttcg ttttgcagcg ttcgtaaacc aatatcctgc
     1801 tccagccgtt cctcaatgta gcattcgata tggcgaacct tgatctgctc cacccgctgg
     1861 gtctggatat tcagcgcccg taaatgttca ctgaaccgtt ttgtaatatg gatacggtca
     1921 ttcaccgttt tgaagctacc gcccgctttc ttcgccagcg ccttcatttc actgcttaat
     1981 ttgctcatga attcccctgc aacaaaaaca aacagtctcc ggcgtgtgct ttttggctgc
     2041 gccagaacga taccttcacg ccggagtggc agtttgttga ggtttaggga gtttcaggct
     2101 gtacgcggta cagactgcct gccgaagcag gggtgtctcc ggggcgtcgt gcccccctcg
     2161 gtatcgatct ttttctcctt tactctgaac tgacagcagc cccaaagggc atgtcagacg
     2221 gcggaaatat ggcgcaggcc agaactggca cggcttgcgg tggtgagttg tgcggcgtca
     2281 ctttgctttt tcgctgacgc gatcaaagtg acgcctcgtc tggtgcagag gattaacgtt
     2341 gcgtttctgg tgcagggtga cggtcacgcg gcagcaaagc ctgacggcaa tgcaggccgc
     2401 agcccgtcaa tgtaaggcgc tggttgtcag cagaagataa aacccggcaa agccagagtg
     2461 tcagcccgta aagccgggta tctggtgcag tgatggtgta tatgtacgtc agtctcagat
     2521 gcaggtatat ttgcatcccc ggcgttttca gagatcaacg ccggtaaagg atgccatgct
     2581 ggcgaagcca ggcgcttctt tcagacgtga agcgacaaag ggactgcccg gaggcagttt
     2641 gttgcaggcg ggagactgcg ccattatttc tggagcagga aggaaaaact gtttccgggt
     2701 ttaaaagatt gtttgcttac ctggtgacgg aaaaactgcc gcgcttcgct tgtcttctcc
     2761 cggcgcgctg ccagcaaaag caggcagcgc gccgggagag tctggtttta ctgtaacagt
     2821 gcgcctgacg gttcagccat caggatcggc gtttttgcat gagtcttccg gtacggttgc
     2881 cccgatcatc gatgtatccg gacggccaga taatttccgg tgatgtttcc agcgcatccg
     2941 caatcagttt tcgccgcgcg gcctggagcg ggtcagcgca tttgccagcg tggaagacac
     3001 cagcccggcc tgcgggaaac ctcagccagc gatgttccct tttttctcag gcccgcgata
     3061 atatcggcgg ggtaccagtc gggttgcatc atgctggcct cccgtcgata acctgtatgc
     3121 cgtcagcagt acgtatccag cgtggggcgt tcagatcggc ggcaaaatac cagaccagtt
     3181 cgcacagcag cccggtcagc gtggtttcgg tttccggcag cagcgcgtgg ccgttcagta
     3241 tctgcgtcag cgccaggcag taatcgcaga gtgatcctgc atcaggctca aaaccgggtg
     3301 aaacatcggg aagatcattc gtaataaacc cgtcaatcag atgtggtggg atcggctcgt
     3361 tcagcgtggg ccgcaacagt gcaagacagg tattaagcct gccacagagc gccgttttca
     3421 gcgccggatc gcggcactcg atgagcgttg tggcaacgcg atcgcagtac tccgccaaat
     3481 cggtgaaatc ggtagcggca ctaaagggaa cagcaagtaa attgcattca gaggtgagag
     3541 tagtcgccat aaggccgtct ccagtgagtg ataaatattc cagccactgg agaggctaat
     3601 ctcacgggtg gtggaaccgg acggggttag ccttaccagc ctcactgaca ctggcgcgtc
     3661 tttcgacgcc cccgcccgat cccaccattg agatgagacc gtgcgcgaca cacaaatatt
     3721 gtatcgtcag tgaggatctt caggaggcta atcccgacgc ctgattttgc aggcgcggcg
     3781 caacaataca atgttcggta atttgcagca agaggtttag cggaagcaga cgcacacgaa
     3841 aaaaacaatc acatcacgaa tattaattat gaatatattt atataaaaaa tatattgaac
     3901 aatatgtaga cactttctaa taaatatcct gccagttaat tcattccgtt gatatcagtc
     3961 aaaaaaataa tacaaaaaga gagtatttcg tttcatacag taacgttatt tatcattttg
     4021 tccggtgccg ccagcatcag gccagatgaa ccataaaagg atatcagcga tgagcggaaa
     4081 accagcggca cgtcagggcg atgccacaat gaaaggtggc ccgatagtcc aggggtcggc
     4141 aggcgtgaga attggtgcgc caaccggcgt ggcctgctca gtgtgccccg gcggattcac
     4201 ttccggcaac ccggtgaatc cgctgctcgg tgcaaaagta cagcccggcg aaaccgattt
     4261 cgcgctgccc ggcccgttac cgttcgtgct ctcccgcacc tacagcagct accggacgaa
     4321 aacccctgcg cccgtcggta tcttcggtcc tggctggaaa gcaccctttg atatccgctt
     4381 acagatacgc gataacgaac tgatactcaa tgataacggt ggcagaagca ttcattttga
     4441 gcacctgttt cccggtgagg atggtttcag ccgcagcgaa ttattctggc tggtacgcgg
     4501 cggcgtggcg aaactgaatg aaagtcaccg tcttgcccca ctctggcagg ctctgccgga
     4561 agaac
//
LOCUS       DAOFQD010000222         4508 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.223, whole genome shotgun sequence.
ACCESSION   DAOFQD010000222 DAOFQD010000000
VERSION     DAOFQD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.223"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..798
                     /locus_tag="QBF46_004643"
     CDS             <1..798
                     /locus_tag="QBF46_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6676730.1"
                     /translation="LLPYVKQRRLTTERLLLAIPPEIGALIKHYVDKAQLLSHDRMFE
                     MGVSAPAFVSQSISQAILNFSPPEYQTAVGRGEAAPPSITSTDLRHNVGHSLAMQGAS
                     AEEIAHILGHSSLVVAKHYIRATPALALIRAKALGSNPVWQNMVAMMLTGKLVPSKEW
                     EGRRVVGMVGDRLHYEIGGCARTDDECPFCEVRCCYGCLYYRPFLDGHHQGVLNSVSK
                     EVDELIAVSDSVGNALNPLISVHETTQIEIKSVIARCHLHNVRGCGK"
     gene            795..3158
                     /locus_tag="QBF46_004644"
     CDS             795..3158
                     /locus_tag="QBF46_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676731.1"
                     /translation="MKKNIENFETFIIEQKAWFEDNLAADFAESWDSFVWICGIKGSG
                     WLRGNGANLLRFDEVNRLKGIDDRHTVSEPYQLFMKAMLVLVYRGRNRSISSAVAVAT
                     LIILKRWYCALIKLTGQTHPIYLTTDVVRSAMDTLSAASRPGDTNLANYKGRCVKIQK
                     LVNHHAFTLVTLQYVSDDCYTNQTNLTRKARETISLKEKDKLDDTSTDGEDTLITIKG
                     FLNIVSLIQRVESGTEKIALNCLLLLIVTGFRSVEAFNLRQDALVKRHIVNSDFSKRL
                     RNKGLPDYFLGIRYVGVKGAGERTHWVEPLAVPLVENIFKSVKLLTAESRKHIEYLRS
                     KKFSDYLPKPISDITGELVELDDIVKYMVQSSSELRGRAGLRDKASKALEKRGFIPAK
                     VILKSGNEKEKYFTKSDLSHFLKSEFGDNSANTPCTHAWAENGKRYEIKYEELLFLFP
                     KGSLTLKRVLQLKATPLPLNNNGLNKFLGNVAGYVSVFSKYRLLEDDGRPTQLRTHIP
                     RHNINTFLAIAEISDHLQAMLMGRVDITQNQHYQHLALKERRKAASLTPLVPTVPEQS
                     AFTAVDVDSPLDMVKQSGLMTFNSTQSLETNIKANLHTFDDRNDVASFIEASSGDGLF
                     EDIAAAFEEISKNEGPLQASEMVQRHAVLHPLKLGSCMRDVNLWGCPYRMKCQALKPC
                     EHFTLTGRIDEYSTIAVKGRALNEASLAFEQYIAALPDNQLIQGNIEENLTHLDALSD
                     QLRRRSNLLQVISAQEILSGEIKVEGEIRTLAQLFALEHHKNKEEEN"
     gene            3158..3601
                     /locus_tag="QBF46_004645"
     CDS             3158..3601
                     /locus_tag="QBF46_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676732.1"
                     /translation="MRGKELDTQIEHELQLMLIEGFDKSPISAKNLHARLKSKGIING
                     GLSTLSNIERRRLIAAYVDQQLSPLNLRPKEKQQYVNRKTRQALLGRNQQLQEENKEL
                     REQLAQNTLSLIEIVKAVKINTVIPVESLLAPHLIMELHKRKKDQ"
     gene            complement(3799..4131)
                     /locus_tag="QBF46_004646"
     CDS             complement(3799..4131)
                     /locus_tag="QBF46_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6676733.1"
                     /translation="MSEKNPIPERLKEARCRAGLSQRSLGLLIGFDPASASSRMNHYE
                     KGRHVPDIDTLRRMAAELNVPLNYFFCDDQTTAELALLISRMTEEERSKLIEALKTSS
                     GDNTADKK"
BASE COUNT         1319 a          950 c          981 g         1258 t
ORIGIN      
        1 ctactacctt acgtcaaaca gagacgcctt acgaccgagc gtttgctgct tgctatacct
       61 ccggaaattg gtgcgttaat caagcattat gtggacaaag ctcagttatt atcccatgac
      121 agaatgttcg aaatgggagt gtctgcccct gcttttgtct cccaatctat aagccaagcc
      181 attctaaact tcagtccccc tgaatatcaa actgccgttg gtcgcggaga ggccgctccc
      241 ccgtcaatca catctactga tctacgtcac aacgttggtc actcacttgc aatgcagggc
      301 gctagtgcag aggaaattgc tcacattctt ggccattcat ctctggttgt agcaaagcac
      361 tacatccgtg cgaccccagc cttggcattg atccgcgcta aagcactcgg ttctaaccct
      421 gtgtggcaaa acatggtggc tatgatgctt actgggaaac ttgtcccttc aaaagaatgg
      481 gaaggtcgac gcgtggttgg tatggttggt gatcgattgc actatgagat tggtggctgt
      541 gcgagaaccg atgatgaatg ccccttctgt gaagtccgtt gctgctatgg ttgcttatat
      601 taccgccctt tccttgatgg ccatcaccag ggcgtattga atagcgtctc taaagaagtc
      661 gatgagctga tagcagtatc agatagtgtt ggtaatgctc tcaatccact aatttctgtc
      721 cacgagacga cgcaaataga aatcaagtca gtgattgccc gttgtcattt gcataacgtc
      781 agaggttgtg ggaaatgaaa aaaaatatag agaactttga gacttttatc atagaacaga
      841 aagcttggtt cgaagataat ctggcagcag attttgcaga atcatgggat agctttgtct
      901 ggatatgtgg gatcaaaggc tccggctggt tacgtggcaa tggggctaat ttattacgtt
      961 ttgatgaggt taaccggctc aaagggatcg atgatcgcca tactgtctcc gagccttatc
     1021 agttgttcat gaaagcgatg ctggtactcg tctatcgagg gagaaatcgc agtatttcct
     1081 ctgcggttgc tgtcgcaacg ctgattattt tgaaacgatg gtactgcgct ctcattaagt
     1141 taactgggca aacacatccc atctatctga ctacagatgt cgtacgtagc gcaatggata
     1201 ctctgagtgc agcttcccgg ccaggtgata ccaacctagc caactacaag gggcgttgtg
     1261 ttaagattca gaaattagta aaccatcacg ccttcacatt agtaacattg caatatgttt
     1321 ccgatgattg ttataccaat cagactaatt tgacccggaa agcccgggaa acaatctcac
     1381 taaaagaaaa agacaagctg gatgatactt ccactgatgg tgaagataca ctgattacca
     1441 ttaaaggttt cctcaatatc gtgtcgttaa tccagcgagt tgaaagcggt actgaaaaga
     1501 ttgccctcaa ttgtcttctg ttactgatcg tcactggatt ccgttctgtt gaggcattca
     1561 atctcagaca ggatgccttg gtcaaacgac atatagttaa ctctgacttc agcaaacgtt
     1621 tgcggaataa aggattgcca gattattttc ttggcatacg ctatgttggt gttaaggggg
     1681 ctggcgaacg aacgcattgg gtcgagcctt tagctgtacc tctggtcgaa aatatattta
     1741 agtcggtgaa gttactaaca gctgagtctc gcaaacatat cgaatattta cgatcaaaaa
     1801 aattttctga ttatttacca aagccaatca gtgatattac cggtgaactc gttgagcttg
     1861 atgatattgt aaaatacatg gtccagtcct catcagagct tcgggggcgt gcaggcttac
     1921 gtgataaagc ttcgaaagca ttagagaaac gtggctttat accggcaaaa gtcatcctta
     1981 aatcaggtaa tgaaaaagaa aaatatttta caaagtctga tcttagtcat tttcttaaaa
     2041 gtgaattcgg tgataacagt gcaaatactc cctgtactca tgcatgggca gaaaatggca
     2101 aaagatatga gattaaatat gaagagctat tgttcctttt ccccaaagga tcactgactc
     2161 taaagagagt attgcaacta aaagccacgc cactaccact aaataataac ggtctaaaca
     2221 aatttctagg taacgttgct ggctatgtat ctgttttcag caaatacagg ttgcttgaag
     2281 atgacggccg ccctactcag ctccgcacgc atatccctcg acacaacatt aatacttttc
     2341 tcgcaattgc agagatctca gaccacttac aagcgatgtt gatggggcgt gttgatatta
     2401 ctcaaaatca gcattatcag cacctggctc tcaaggaacg gcgaaaagca gcttctctaa
     2461 cccctttagt tccaaccgta ccggagcagt cagcatttac tgctgttgac gttgattcac
     2521 cgctcgatat ggtaaagcaa agcggtttaa tgacgtttaa tagtacccaa agtctggaaa
     2581 ccaacataaa ggcaaacctg catacctttg atgatcgtaa cgatgttgct agtttcattg
     2641 aagcgtcgtc aggggatgga ttattcgaag acatagccgc tgcgttcgag gaaatcagta
     2701 aaaatgaagg gccattacaa gcgtctgaga tggtacaaag gcatgcggta ttgcatccat
     2761 taaaactggg ctcctgcatg agagacgtta acttgtgggg atgcccttat cgaatgaagt
     2821 gccaagcatt gaagccctgt gagcatttca cgttgacagg tcgtattgat gaatactcaa
     2881 ctattgcagt caaaggtcgg gcattaaacg aggcttcgct cgcttttgaa cagtatatcg
     2941 cggcccttcc agacaaccag ttaatacaag gcaatatcga agaaaatcta actcacctgg
     3001 atgccttaag cgatcaatta cgcaggcgct caaacttgtt acaagttatc tctgctcaag
     3061 agattctgtc tggagagata aaggtggaag gtgaaattcg tactttagct caattatttg
     3121 cccttgagca ccataaaaac aaagaggaag aaaactaatg cgtggtaaag aattggatac
     3181 tcagatagag catgaactcc agttgatgtt gattgaaggg tttgataaat cgcctatttc
     3241 agctaaaaac ttacatgcca gacttaaatc aaaaggaatc attaatggtg gcttaagtac
     3301 attaagtaac attgaacgaa ggcgtcttat tgcagcctat gtcgatcaac aactatcgcc
     3361 tctgaatctt cgtcccaaag aaaaacagca gtatgtgaac cgtaagactc ggcaggcctt
     3421 gcttggtcgt aatcagcagt tacaggaaga gaataaagag cttcgcgaac aactagcaca
     3481 gaataccttg tcattgattg aaattgtcaa agcggtaaaa atcaatacgg ttataccggt
     3541 agaaagcctt cttgctccgc atttgatcat ggaattacac aaaaggaaaa aagatcaatg
     3601 atagatatag cggtatattc gcactacgaa ctcatgaata aacaccttgc tctattcgaa
     3661 agatgagcat tgcaactttc aacgcccact cattagagtc cattttctca gagggcgtct
     3721 actactgtaa tgccctgctt ggtttattgc actgcacttt tgttaccttc aaaaagttta
     3781 gcaggcgttt gtacaacatc attttttgtc agcagtgttg tcacctgaag acgttttgag
     3841 tgcttcgata agtttacttc gctcttcctc tgtcattcgg gatatgagta atgcaagttc
     3901 tgctgtcgtc tgatcatcac aaaaaaagta gttcaacggc acgtttaact ctgccgccat
     3961 ccgtcgaagc gtatcaatgt cggggacatg gcgccctttc tcatagtgat tcatacgact
     4021 actagccgat gcgggatcaa agccaataag tagccccaga gagcgctgtg atagtcccgc
     4081 cctgcatctc gcttctttaa gtcgttctgg tatcgggttt ttttctgaca ctgaatcatc
     4141 ttcatcggcc tcggatgctt agattgtcta agtatctgtc attgttgtat acttagcaat
     4201 cctaagttaa cagccttttt ttagtgatat gaatagtaga gtaaccaacc ctgagtcgta
     4261 catattttct gcaatcatct atattggcaa agataacttc accagtaacg aagtagcgaa
     4321 aattcttatc gagcgatttt cacttccgaa aaattattta aaggcaaaag cttttgctta
     4381 taaccaaatt caaggtctgg ttagaaaggg attgttaaat aaagtaagaa aattaggtgc
     4441 gtatcaatat ttatactcag ccacatctga attcaatgtc gtaaaggaaa acgtagagtt
     4501 aattgagg
//
LOCUS       DAOFQD010000223         4494 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.224, whole genome shotgun sequence.
ACCESSION   DAOFQD010000223 DAOFQD010000000
VERSION     DAOFQD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.224"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(232..990)
                     /locus_tag="QBF46_004647"
     CDS             complement(232..990)
                     /locus_tag="QBF46_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDQ6676734.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLAVHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(998..2101)
                     /locus_tag="QBF46_004648"
     CDS             complement(998..2101)
                     /locus_tag="QBF46_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDQ6676735.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHKRFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSPVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQYLEKRFNTGRTPH"
     gene            complement(2111..3292)
                     /locus_tag="QBF46_004649"
     CDS             complement(2111..3292)
                     /locus_tag="QBF46_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDQ6676736.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3360..4385)
                     /locus_tag="QBF46_004650"
     CDS             complement(3360..4385)
                     /locus_tag="QBF46_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6676737.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1231 a         1184 c         1066 g         1013 t
ORIGIN      
        1 catcaaataa aacaaaaggc tcagtcgaaa gactgggcct tttgttttat ctgttgtttg
       61 tcggtgaaca ctctcccgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
      121 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccagaca tcaaatcaag
      181 cgaaaggcca tccgaaagga tggccttttt gcttttcgaa ctaacattca attaatggat
      241 tacctgcgat aaaaatgccc tcgtacgctc tgatttagga tgcgcaaaaa attcatcagg
      301 tgcagcttgc tccactattt ccccacgatc cataaaaatt acccggtcag cgacggttcg
      361 tgcaaacccc atctcatgtg ttacacacaa cattgtcata cccgactgcg ccagcccaat
      421 catcgtatcc agcacctctt tcaccatctc aggatcgagc gccgacgttg gctcatcaaa
      481 caacataatt ttcggcttca tacacagcga acgcgcaatg gcaacgcgtt gctgctgacc
      541 acctgaaatc tgtccgggaa acttatgcgc atgttcggca attctcaccc gctctaggta
      601 atgcaccgcc agatcttcag cctctttctt aggcatcttg cgtacccaaa tcggtgccag
      661 ggtacagttc tgtaaaacgg tcagatgagg gaagagattg aaatgctgaa agaccattcc
      721 cacttcctgc ctgacgcgct caatattgcg gatatcttca ttaagttcga tgccatctac
      781 cacgatccgt ccctgttgat gttcttccag atgattaata caacgaatgg ttgtcgattt
      841 accggaacct gaagggccac acagaacgat ccgttctccc ggttgcacgg ttaaatttat
      901 atttttcaaa acatggaatt gtccatacca tttattgacg ttttccagcg taatcatcgc
      961 gttagcaggt tgcagtaaaa tttggctcat agtgtcctca atgcggtgta cgcccggtgt
     1021 taaaacgttt ttccagatac tggctatagc gcgacatgct gaaacagaag atccagtaga
     1081 tcagcgcggc gaaaacatac ccttccgtcg acatacccaa ccaggcggga tcaacggttg
     1141 cctgctgaac gctactgaaa agatcgaaca acccgataat gatcaccggg ctggtatctt
     1201 tgaagagtgc gatgatggta tttaccagcc caggaattac cagcttcaac gcctgtggca
     1261 gaataaccag cccctgagtt ttccagtaac ccaacgccag cgactctgcc gcttcatatt
     1321 gccctttagg cagcgcctgt aatccacctc gcacgacttc cgcaacatat gctgactgaa
     1381 acaggatcac gccaaccagc gcgcggatca atttgtcgat actggtgcct tctgccataa
     1441 acaacggcag catgaccgaa gacataaaca gaacggtaat cagcggcacg ccgcgccaga
     1501 attcgataaa aatgaccgat aagatacgca caatcggcat atgggagcgg cgacctaacg
     1561 ccagtaagat cccccacggt agcgcccccg caatcccaac tgatgcaata attaaagtta
     1621 gcgtcagccc gccccattgc cgggtttcaa cccgctcaag agcaaaaaaa ccgccataca
     1681 tcagccacca gacaatcagt gggtaaatca ccgcccaggc cgcaatatag cgaccgcgat
     1741 gcgggagtat tttccagaac attggtgcga tcgacacaag cccaatcagt aatgcgaggt
     1801 taattcgcca gcgttggtcg tgtgggtaaa gcccatacat aaactgacca aatcgtttgt
     1861 ggatgaagac ccaacaggca ccggcttttg tgcagtcggc acgcgttgag ccaacccagt
     1921 tagcctgcaa aaatgcccag ttcagcaacg gtgggatcag ttcccacatc aaccatatgc
     1981 agccaatagt cagcaggcta ttgctccagc tggagaacag attttttcgc acccacacca
     2041 tcgcgcggct tgagttatgg ctcgccgggc gcgggggatg agacagcaat acttttgtca
     2101 tcatgactcc ttagcgttca acgatcgcga tgcggcggtt atagatattc atcagcagcg
     2161 agatagtcag gctgataatc agatagaccg acatggtcat ggctatcgtc tcgatggctt
     2221 gccccgtctg attcagcacg gtgccggcaa acagcgaaac catatcggga tagccaatag
     2281 cggcggcaag agaggagttt ttgacgatgt tgagatactg gctggttaac ggtggaataa
     2341 tcacccgcaa tgcctgggga ataatgacct ggcgtagcgt aaccgggttg ggtaatccca
     2401 gtgaccgagc cgcttcatgt tgaccataag gcactgcctg gatcccagcg cggataatct
     2461 cggcgataaa tgcagatgta taaaccgaaa gtgccagcgt taaggctgcc agttcaggaa
     2521 ttaaaaccat cccgccgcgg aagttaaagc ctcgtagggc tgggacatcc cagtgtagtg
     2581 ctgcgccaaa aagccattgc gccagtaaag gcaaaccaat gatcaacact gcggcgatcg
     2641 gccaggtgcg gcgcagttgt ccggtcttta tctggtatgt tttattaaag cggaatagcc
     2701 caacagaaag gactatagcc ataacaacag ccaggataaa cgcaataaat ccgtctccca
     2761 gctgcggtga cggaatataa agcccacgat tgctcaaaaa ggccagatca aacgcgctca
     2821 cagcctggcg tggtccgggc aaattgcgca acacggcaaa gtaccagaag aagatttgca
     2881 gcagcggggg aatattacgg aagatctcaa tataaattgt ggaaagcttt cgtagcagcc
     2941 agttatccga aagtctcgcc agaccgataa agaagcccaa aacagaagcg aacacaatac
     3001 acaatgcaga aaccagtagc gtattgagta agccgacaat aaaaacgcgt ccgtaggtgt
     3061 cgccctgctg gtaatcgatc aaatgctgga caataccgaa gccagcgccg cgatccagaa
     3121 aggcaaaacc tgaagtaatg ccacgattat tgagattcgt tacagtgttg tgaaataacc
     3181 aaccaacaat gccgacaaca gcaacaacgg caaggatctg gaataaccag gcgcgaaccg
     3241 tagggttggc aaaagagagt gagcctttaa cggttgagcg gcgatgagac atagaaacct
     3301 cggaaagcat aacgctgaag agagcgccgc cgtagcgacg ctcgttacct cacaccgact
     3361 taacgcacgg gcggtgcgta ctgaataccg ccgttattcc agagattatt ttgcccacgt
     3421 ttaattttca gcgggctttc tgaacctacg ttacgctcaa aaatttccga gtagttaccc
     3481 acctgtttga tgatgttata ggcccattta ttatccaact tcagatcctt gccgtaatcg
     3541 ccttctttac ccagcagatg tgccatatca ggcgttgctg gattagccgc tttttcatcg
     3601 acgttctggg aattgatgcc catctcttca gcatttagca tggcgaaaag cgtccagcgt
     3661 acaatcgaga accattcatc atcgccacga cgaactaccg gcccaagcgg ttctttagag
     3721 ataacctccg gtaagacaat ccattcagcc gggttgctta atttgatgcg cagggcatac
     3781 agttgggatt gatccgaagc cagcgtatcg cagcgaccag attccagtgc cttcgctgat
     3841 tcgtcagagc gatcgaaagt cactggtgtg tacttcatat tgtttgcctt gaaatagtcg
     3901 gcgacgttga gttcggtatc agtacccgcc tgaatacaga cggtagcgcc atccagttct
     3961 ttcgcgcttt ttagccccgc tttatcgtgc gtcaggaagc caatgccgtc gtaataagtg
     4021 acgccagtaa atgccattcc catcccggca tcgcgagatg aagtccaggt cgtattacgg
     4081 gagagcaaat ccacctcccc tgactgtaaa gcggtgaagc gttcttttgc agtgagcggg
     4141 gtatatttca ctttcgtgtc gtcaccaaat acagcagcgg caacaccacg acaaatatca
     4201 acatcaatac ctgaaaattt accgtcagca tcggcataag agaacccagg taatccatca
     4261 ctgatcccgc attgcacaaa accttttttc tgtacggcat caagcgtcgc gccagcatgc
     4321 gcctgatttg caacggcaag cagcacgctg gcggcagcca gtgtggctat catcatcttt
     4381 ttcataatgc ttcctgtgag gcgaaaatta tcgttatagg acgtttggga aacgtctttt
     4441 acctgtctgt ggccatcctc ttataagcaa aagtagtgcc agcttttgcg cacg
//
LOCUS       DAOFQD010000224         4431 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.225, whole genome shotgun sequence.
ACCESSION   DAOFQD010000224 DAOFQD010000000
VERSION     DAOFQD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.225"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(124..516)
                     /locus_tag="QBF46_004651"
     CDS             complement(124..516)
                     /locus_tag="QBF46_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676738.1"
                     /translation="MKYILTVLCIFFCQTASAYPYRIYTAPEGAIVKNILTNEVIGKT
                     PVEVDVSNTEAGSTFGISMYRHENVAIKIFTVMPNAENNFTVSGPDVATMSLPGKAPL
                     NVTNDSNAAAVHIDLRPFLSEPAHHPVQ"
     gene            complement(513..1034)
                     /locus_tag="QBF46_004652"
     CDS             complement(513..1034)
                     /locus_tag="QBF46_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676739.1"
                     /translation="MIRVSAFILTLLVTGCQSVGSKIAVLPSVGFDPIMSNRTEAYTD
                     GKVTFLIESSGTDVWLLAKNGTKEFIELSDLNLGGSRCTYSSRGKQLISPSSVTIFTV
                     PTVGLLGLCYDNNDQLTFINNSFKNISQSSRDGLTLPLLFSIKYKFPGSFDSKQIVVT
                     QSFDLEFLQKEQS"
     gene            complement(1027..1866)
                     /locus_tag="QBF46_004653"
     CDS             complement(1027..1866)
                     /locus_tag="QBF46_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676740.1"
                     /translation="MLKLELIILPLLAFLGPSNITNASTKTPIPSSQLSDAKTGVNYE
                     FITIAANYVNGMWLVDGRQRPVIKTSMTKRNYLQIENDSASTPLNLVIPKIEFSIIAK
                     NGVFISKFISLDEDASGKRILWLEPGSSMTISFVNDLSSTPLQALVSVTKQKKEVIAI
                     FGPDQGKKFTLPLPSDSQQSSIDYVPSPEIALRINAPKKLQPAIAIPEDASASLLPSH
                     TYKIRAAEKVRTFKFSSFVWVGKRNNLLGGSDYFSKELKIYPGENFDISTIQKTTMRT
                     END"
     gene            complement(1866..2813)
                     /locus_tag="QBF46_004654"
     CDS             complement(1866..2813)
                     /locus_tag="QBF46_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676741.1"
                     /translation="MASEHVQTIDLTNFSGRVFFLADPHGHYSTLCNLIHSISEPDEE
                     LIVFSTGNLFDYGPEPMELMTAINTGIFDGRSVRVFSAAGAGEEMLKKLLPINKDRRT
                     YYPSTFLNERWCARGGRWHKQINRNYLEEEICKLLSTQLATVMKVLFKGNISIGICPS
                     DYTDIRQGFNNTYNALLAFNQANVNIFQSQFLYGMDHAVRPVNVSDVNLVVLGRNPVN
                     SIRKAHGLPLTNLPVLIGNCLHINTGSLYMSEISSPALLAPGIPPTTVPAITLVELIL
                     ASTPTLICHQMILNKNGIYTQNTAPLNLDSNDNNIEATL"
     gene            3187..4221
                     /locus_tag="QBF46_004655"
     CDS             3187..4221
                     /locus_tag="QBF46_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stbA family protein"
                     /protein_id="HDQ6676742.1"
                     /translation="MTELAKTKLAVAESNSTELETLKVVIDDGSKAAKLVCTNNQGDL
                     VPLLTQNSFVADFRVSHDGLIPFNYLIDGLQRFSHHSESSNALETTDVAHQYDEISRL
                     NVHHALHSSGLMPQDVHLYVTLPLSQFYTALGETNNENIQRKKDNLMKPVERYIDGKR
                     VSFNVVSVTVFPESLPAVTRADEIESIESFESSLVIDLGGTTLDVASITGQLEQISSV
                     RGFDRIGCSIVYDEIRRYLDSAKLNASNAYIHHLVDNRENKASLKVSPEDLDGVFNAV
                     NGAVAQLQEKVIKAVTQVEERPHNVFLVGGGSYLIEPAVRKHFDKSKIIMVDNPQFAL
                     SLAIADTVFA"
BASE COUNT         1280 a          848 c          970 g         1333 t
ORIGIN      
        1 gtctacgcat taccaagtcc taaaagaaat caatagcaaa aaatatctta actatctgat
       61 aatgatgggt tttaacatat acggtattgt taagcaggcc ccaaagattg gggcctagaa
      121 gtgttattga acagggtgat gggccggttc agacagaaaa ggtcttagat ctatatgaac
      181 cgctgctgca ttactgtcat tggtcacgtt taacggggcc ttacccggta aagacatagt
      241 ggcaacgtct ggaccagaaa cagtaaaatt attttctgca tttggcatta ctgtgaaaat
      301 tttaatagct acattctcat gacgatacat tgaaattccg aaagttgatc cagcttcagt
      361 attgctgaca tcaacttcaa ccggtgtttt cccgatcact tcattagtaa gtatattttt
      421 cacaattgca ccttcagggg cagtgtaaat gcgataaggg taagcacttg cagtctggca
      481 gaaaaaaata cacaaaacgg tcaatatata tttcatgatt gctccttttg caaaaattct
      541 aaatcaaatg attgagtgac tacaatttgt ttggaatcaa atgaacctgg gaatttatat
      601 ttaatactga acagtaatgg caatgtgagt ccatctcgtg aagactgtga gatgttctta
      661 aatgagttat taataaatgt taattggtca ttgttatcgt aacaaagacc aagtaaacca
      721 actgtgggca ccgtaaaaat tgtaactgaa ctaggtgata tcaactgttt tccacgtgac
      781 gaataggtgc aacgactccc gccgagattc agatcagaca actcaataaa ttctttggtc
      841 ccattcttgg cgagtaacca gacatcagtg cctgaactct caatcagaaa agtaactttt
      901 ccgtcggtgt aggcttcagt tctgttgctc attatagggt cgaaccctac cgaagggagg
      961 actgcaattt tgcttccaac cgactgacat ccagtcacaa gtaaggtaag aataaatgct
     1021 gaaactctaa tcattttcag tcctcatagt cgttttctgt atggtactga tatcgaagtt
     1081 ttctcctggg taaattttca actctttgct gaaataatct gaaccaccga ggaggttgtt
     1141 acgctttcca acccaaacga acgacgaaaa cttaaaagtc cttacttttt ctgccgctct
     1201 gatcttgtag gtatgtgaag gtaataaaga tgcactagcg tcctccggga ttgcgatagc
     1261 gggttgaagt tttttggggg cattgatacg taatgcgatt tctggagagg ggacatagtc
     1321 gattgacgat tgttgtgaat ctgacggtag gggcagggtg aattttttac cctgatctgg
     1381 gccaaaaatg gcaataacct cttttttctg ctttgttacg gacactaacg cctgtaacgg
     1441 ggtactggat agatcattga cgaatgaaat tgtcatgctg gaaccaggtt ctaaccagag
     1501 aattcgtttc ccggatgcat cttcatcaag ggagataaat tttgatataa acacgccatt
     1561 ttttgcaatg atggaaaatt caattttggg tattacaaga tttaaaggag ttgaagcaga
     1621 gtcgttttca atctgaagat agttacgctt agtcatgctt gttttaatta ctggtctttg
     1681 tcgaccatca acaagccaca ttccgttgac gtaatttgca gcaatagtaa taaattcata
     1741 gttaacccca gttttggcat cagatagctg ggacgacggt atcggggttt tagtcgatgc
     1801 atttgtaatg ttggatggac ctaaaaatgc cagcaacggc aaaattatca gttctaattt
     1861 aagcattata atgtggcctc tatattatta tcatttgaat ccaggttaag tggtgcagtg
     1921 ttttgtgtgt aaataccgtt tttattcagg atcatttggt ggcaaatcag ggtcggtgta
     1981 gatgccagga tcaactcgac cagtgttatt gccggaactg tagtaggcgg aatacctggt
     2041 gccagtagtg ctgggctact gatttctgac atgtacagag aacctgtatt gatgtggagg
     2101 cagtttccta tgagtaccgg taagttagtc agcggtagcc catgggcctt tctgatgctg
     2161 ttaactggat tccgacccaa tactacgagg tttacatcgc tgacattcac tggcctgact
     2221 gcgtgatcca tgccgtatag aaactgactc tgaaaaatat tcacatttgc ctgattaaaa
     2281 gcgaggaggg cgttatatgt attgttgaag ccctgccgta tgtctgtgta atcggatggg
     2341 caaataccta tagaaatatt gcctttaaaa agcaccttca tcacagtagc cagctgggtg
     2401 gagagcagtt tgcagatttc ctcttcaaga tagttgcggt taatttgttt atgccatcgc
     2461 ccgcctcttg cacaccaccg ttcattaaga aatgtgcttg gataataagt cctgcggtct
     2521 ttatttatgg gtaagagctt tttcagcatc tcttctccgg ccccggcagc tgagaacaca
     2581 cgaacagaac ggccgtcaaa aatccctgtg ttgatcgctg tcattaactc cataggctca
     2641 ggtccataat cgaagagatt tcctgtggag aaaacgatca actcctcatc aggttctgat
     2701 atcgaatgga taaggttaca tagcgtcgaa taatgaccat gagggtctgc cagaaagaaa
     2761 acccttcctg aaaaattagt cagatcaatt gtctggacgt gctcggatgc cataaatgcc
     2821 tctttctgaa attgcattaa tactaccgga tgaaagtcat caattttttc gataagaggg
     2881 gtaatttttg tgagtgtcca aaatggagct agaaatcatt tttcggtttc cgagccgtat
     2941 caactgtaat gcacctcagt tgttttattt ttggtataaa aacgctgagc aaaatatagt
     3001 ttttgcgtgt gttgctcagg tgtacatgaa tctagggttg tatctggatt aaaactgaag
     3061 gattcttttt taacaaggac ttacgttagt gttttgtccc gtttaatgtt gacctaaagc
     3121 aaccattggt gtataaattc cataacaaaa gtggtgggct aaataaacca atacgagggt
     3181 ttctgcatga ctgaattagc aaaaacaaaa ctggctgtgg ctgaatcaaa ctctactgaa
     3241 cttgagacac tcaaagttgt tattgatgat ggtagtaaag cggcaaaact ggtttgcact
     3301 aacaatcaag gtgaccttgt acctttattg actcaaaata gtttcgttgc agatttccgg
     3361 gtaagccatg acggacttat tccattcaat tatctaattg atggtcttca acgattctcg
     3421 caccacagcg aatcaagcaa tgctttagaa actaccgacg ttgcgcatca gtacgatgaa
     3481 atcagtcgtc ttaatgttca ccatgctcta cattcctcag gtctaatgcc acaggatgtt
     3541 catctctacg taactttgcc actcagtcag ttttataccg ctctgggtga aaccaataat
     3601 gaaaacatcc aacgaaaaaa agacaacctg atgaagccgg tggagcgtta tattgatggt
     3661 aaacgtgttt catttaatgt tgtttcagtt accgttttcc cggaatccct tccagcggta
     3721 acgcgtgctg atgaaatcga atcaattgaa tcttttgaat caagcctggt tatcgattta
     3781 ggtgggacaa cacttgatgt tgcaagcatt acaggtcagc tggaacagat ctccagtgtc
     3841 aggggttttg accgtattgg ttgctcaatc gtatatgatg aaatccgtcg ttatttggat
     3901 tctgcaaagc tgaatgcaag taacgcctat attcatcacc tagtcgataa tcgtgaaaac
     3961 aaagcctcgc tcaaggtgtc cccggaagat cttgacggcg tgttcaatgc tgttaatggg
     4021 gctgtggcgc aacttcaaga aaaagtgatt aaagcagtca ctcaagtgga agaacggccg
     4081 cataatgttt tccttgttgg tggaggttct tacctgattg aaccggcagt tcgtaaacat
     4141 tttgacaaat cgaaaatcat catggttgat aatccgcaat tcgcactttc actggctatc
     4201 gcagatactg tttttgcatg atgatggttt tgcatgatga tggttttgca aaggggagag
     4261 ttttatgact aaaaaatctg agaaggaaaa cgatcgtatt cagattagtg cgttttggtt
     4321 atcggaacga caatctccgt atgcatataa ttttttgaaa aaaaatgccc taacacatcg
     4381 tggcgagcag atttctctaa taagatctgc aatcactacg gggttagtgc t
//
LOCUS       DAOFQD010000225         4424 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.226, whole genome shotgun sequence.
ACCESSION   DAOFQD010000225 DAOFQD010000000
VERSION     DAOFQD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.226"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..521
                     /locus_tag="QBF46_004656"
     CDS             <1..521
                     /locus_tag="QBF46_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDQ6676743.1"
                     /translation="VTTVERKQEAPTWTPTPNTRREYAKRGESLPAFVPAGPDNPMGL
                     YAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKYLFDNVPVGTRVQIIDQPVKY
                     TTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLRAFINGQEVDVNLANAALQRR
                     SGMPVNINRNGF"
     gene            695..1660
                     /locus_tag="QBF46_004657"
     CDS             695..1660
                     /locus_tag="QBF46_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676744.1"
                     /translation="MTKQKKNSRWQGYDPRYIYSLVVRRYFADMKTKIEIAEELGVSR
                     FKVARLIDEAIEQDYVRFIFPKQQAMDEEIANNLRKKFHLEDAIVLSVAESWTTQEEL
                     NHKLGEVTAEYLMQSLHEDMKVGIAWGRVLSSTVSKLSKLPPLDVVQLSGVHPGIEFS
                     QGPIDLIHKIAAISQGKAHPMYVPMWVDDEELAARLAGDPAVLDTQQYYSQLDVVITG
                     IGDWKSGSSSLCKIFPDTWCEALFQQDIAADVCISLVSREGKILHSPIERLGFGISTD
                     QLQKAKKVIGVAGGEEKYEGILASLKSGLLNVLITDFDTAIKLLD"
     gene            complement(1706..2170)
                     /locus_tag="QBF46_004658"
     CDS             complement(1706..2170)
                     /locus_tag="QBF46_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDQ6676745.1"
                     /translation="MKFEILVNDVDNSISDYQTAITFAGTQLLEKGYITAEYIDACLE
                     REKSYPTGLMMANGQGIAIPHADYTLVNVNSISIVRFANDVTFGQMEDADLTVDCSIM
                     FNLAFATSDQHMSILRRLFTLFQDNAFVETCRNLPATRVAKYVEDTLAASLN"
     gene            complement(2181..2465)
                     /locus_tag="QBF46_004659"
     CDS             complement(2181..2465)
                     /locus_tag="QBF46_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDQ6676746.1"
                     /translation="MKKILVVCGNGIASSSIMVSVLQDFLKEEKIEAQVDKSSLMACT
                     SDTFNSYDLVVSSTKLDNPAITTRVIVGAGLLTGLGEDEIFAAVKEEMLK"
     gene            complement(2489..3859)
                     /locus_tag="QBF46_004660"
     CDS             complement(2489..3859)
                     /locus_tag="QBF46_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDQ6676747.1"
                     /translation="MELITQFINDLGNFIFIPVIFLVLMKILGRPLSECISSAIKVGI
                     GFIALTMTIKLMLEKMQPAVTGLAEATGSSLSAIDVGGAATAVMGFGSSMGAIIIPLC
                     VAVNIAMLVARLTDCVNVDVFNLHQNASMGAIVGVYSGSFLYGALTAALFHVWALIAA
                     DLGAKNNEKFFNLPKGVAISHPVANTYLLFAYPFNWIYDRIPGFRNLNVTAETIQKRF
                     GILGDPTMVGFIIGILLGFCGYGWKSPYHTIIASLQLGMYLAAVMLLLPRMTSIMMEG
                     LVPLSNVARKKLVKRFPDRQITVGMDTALIVGHPSVIAPALLLIPVIVILAVILPGNR
                     VMPLGDLSQFVFFIACMVPVFNGNIIRTWVTSIILFGGGLYIASWMAPATNEVFQKFG
                     TNPDASVMYSSLNPSANPFTGLFAALSHVGIIGYVMAGILLLSIGYLIKQKSRRQIET
                     DLEKAL"
     gene            complement(3863..4399)
                     /locus_tag="QBF46_004661"
     CDS             complement(3863..4399)
                     /locus_tag="QBF46_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDQ6676748.1"
                     /translation="MKTKIAIASDDVGFARKEEIKKYLIEEKNMEIVYDPVTCKEQGV
                     NNFARLADEMAGIIQRDECRLGIYICGTGIGFTCQINKHWGIRAVAVTNPYSAKRARL
                     SNNAQVIGLGCRVNDLEYSKMIVEAWLDNPFDFTTARENSKKNLLEAERNDNALLVKP
                     ADVRWNMGFRPDDEKSEG"
BASE COUNT         1238 a          972 c         1029 g         1185 t
ORIGIN      
        1 gggtgactac cgttgaacgt aaacaagaag ctccaacctg gacgccaacg ccgaacactc
       61 ggcgcgaata tgcgaaacga ggggagagtt tgcccgcatt tgttcctgcg ggtcccgata
      121 atcccatggg gctgtacgcg atttatattg gcaggttgta tgccatccat ggtaccaatg
      181 ccaattttgg tattgggctc cgggtaagtc agggctgtat tcgtctgcgc aatgacgata
      241 tcaaatatct gtttgataat gttcctgttg ggacgcgtgt gcaaattatc gaccagccag
      301 taaaatacac cactgaacca gatggctcga actggctgga agttcatgag ccgctgtcgc
      361 gcaatcgtgc agaatatgag tctgaccgaa aagtgccatt gcctgtaacc ccatctttgc
      421 gggcgtttat caacgggcaa gaagttgatg taaatcttgc aaatgctgcg ttgcaacgtc
      481 gatcgggaat gcctgtgaat atcaaccgta acggattttg acattcaaaa cgtctggttt
      541 gtcggtctgt gtaagcattc cgtcatccag aacaggttgt ttcgaatcat acaaatgata
      601 atttttttct catatgcgca tctcttatga gcataatcgt tatactcttc tgatttaaac
      661 atgtttgctg atgacatgat caatggaaaa gagaatgacg aaacagaaaa aaaacagccg
      721 ctggcagggt tacgatcctc gctacatata ctcgctcgtg gtcagacgtt attttgcgga
      781 tatgaaaacc aagattgaga ttgcagagga gcttggcgtt tcccgcttta aagttgccag
      841 attgatcgat gaggcgatcg aacaggatta cgtgaggttt atcttcccta agcagcaagc
      901 gatggatgaa gaaatcgcca ataatctacg gaaaaaattt catctggaag atgcaattgt
      961 tctttcagtt gctgaatcct ggacgacgca agaagaactc aatcacaaat tgggtgaagt
     1021 caccgctgaa tatcttatgc aatctcttca tgaagatatg aaagtgggga tcgcctgggg
     1081 acgtgtatta tcaagcacgg tcagtaagtt gagcaagttg cctcctttag acgttgtgca
     1141 gttatctggc gtacatccgg ggatcgagtt tagtcagggg ccaatagatc ttattcataa
     1201 gatcgctgcc atttcgcagg gaaaagcgca cccaatgtac gtgccgatgt gggtcgatga
     1261 cgaagagctt gctgccagac tggcaggtga tcctgcggta ttagatacac agcaatatta
     1321 ctcacagttg gatgtggtta tcaccgggat aggtgactgg aaatcaggtt cttcaagctt
     1381 gtgtaaaata tttccggata cctggtgcga agctttgttt caacaagata tcgctgcgga
     1441 tgtgtgtatc tcgttggtca gcagggaagg gaagattctt catagtccta ttgaacgtct
     1501 gggatttggc atttcgacgg atcaactaca aaaagccaaa aaagtgattg gtgtcgctgg
     1561 aggagaagaa aaatatgaag gcattcttgc ttcgctgaaa tctggacttt taaatgtctt
     1621 aattactgat tttgatacgg ccattaaact tctggattaa tagccatatc agtgaagaaa
     1681 gggcagtacc gctgattctg cccttttaat ttaaggacgc tgcgagcgta tcctctacat
     1741 atttagccac gcgcgttgca ggtaaattgc ggcaagtctc aacaaaggcg ttatcctgaa
     1801 acagtgtgaa taaacgacgc aatatcgaca tatgttgatc actggtggca aatgccaggt
     1861 taaacataat gctacaatca actgttaaat cagcatcttc catctgtccg aaagtgacat
     1921 cattggcgaa acgaacaata ctgatactgt ttacatttac cagcgtgtaa tcggcatggg
     1981 gaatggcgat accctgaccg tttgccatca ttagtcctgt cggatagctt ttttcacgtt
     2041 ccaggcaggc gtcaatgtat tctgcagtga tgtagccttt ttccaataat tgcgtaccag
     2101 caaaggtgat cgccgtttga tagtcactga tgctgttatc aacatcattc accagaattt
     2161 caaatttcat tgcacacctg ttatttcaac atctcttctt taactgcagc gaaaatctca
     2221 tcttcgccca gaccagttag taatccagcg ccaacgatga cacgagtagt aatggccggg
     2281 ttatcgagtt tggttgatga aaccaccagg tcatagctgt tgaaggtatc gctggtgcat
     2341 gccattaacg aagatttatc gacttgcgct tcaattttct cttctttcag gaagtcctgc
     2401 agtacagaga ccatgatgga agatgaggca attccgtttc cgcaaactac taagattttt
     2461 ttcatttgat tattcctctg taaaagattt aaagcgcttt ttccaaatcc gtttcaatct
     2521 ggcgacgtga tttttgttta attaagtatc caatagataa caaaaggata cctgccatca
     2581 catagccaat gattccaaca tggctcaggg cggcaaacag accagtaaat ggattcgctg
     2641 acgggttaag cgaagagtac atcacgctgg catccgggtt tgtaccaaac ttctggaaga
     2701 cttcgttggt agccggtgcc atccatgatg caatatacag accaccacca aacaaaatga
     2761 tcgaggtcac ccaggtgcga ataatgttgc cattgaaaac aggtaccatg caggcaatga
     2821 aaaacacaaa ctgagagaga tcacccagtg gcataacgcg gttgccgggc aagatcacgg
     2881 cgaggatcac aatcaccggg atcagcaata atgcaggggc gataactgat ggatggccca
     2941 caatcagagc tgtgtccata ccaacagtga tttgacgatc cgggaaacgt ttgaccagtt
     3001 ttttgcgtgc tacgttggaa agcggaacaa gcccttccat catgatagag gtcatacgtg
     3061 gcaacagaag catgactgca gcaagataca tccctaactg taggctggcg attatggtgt
     3121 ggtatgggga tttccagcca taaccacaaa agcccaacaa aataccaata ataaaaccaa
     3181 ccatagttgg atcgccgaga atgccaaacc gtttttgaat agtttcggcg gtcacattca
     3241 gattacggaa gcctgggatg cgatcataaa tccagttgaa tggataagcg aaaagtaagt
     3301 aggtattggc aaccgggtga gagatcgcaa caccttttgg caggttaaag aatttttcgt
     3361 tatttttagc accaagatcg gcagcgatca gcgcccatac atggaatagc gcggcggtca
     3421 atgcgccata caggaagcta ccagaataga cgccaacaat tgcccccatt gacgcatttt
     3481 gatgaaggtt gaaaacatca acgttaacac agtcagtcag acgggcgacc agcattgcaa
     3541 tatttaccgc aacacagagg ggaataatga tagcgcccat gctggagcca aatcccataa
     3601 ccgcagtcgc tgcgccacca acatcgatgg cactgagcga ggaacctgtt gcttctgcta
     3661 atccggtgac tgccggttgc attttttcca gcatcagttt gatggtcatg gttagcgcaa
     3721 tgaaaccaat gcctactttg atggcagacg agatacattc agaaagagga cggccaagta
     3781 tcttcatcag taccaggaag atgaccggga taaatataaa atttcccaga tcgtttataa
     3841 attgcgtgat cagttccata cattaaccct cagatttttc atcatctggt cggaaaccca
     3901 tattccagcg tacatcagcg ggcttaacca gcaatgcatt atcgttacgt tcagcttcta
     3961 gtaagttctt tttagaattt tctctggcgg tggtgaagtc gaatgggtta tccaaccatg
     4021 cttcgacaat cattttgctg tattcgagat cgttgacccg gcaacccagt ccaataactt
     4081 gtgcgttatt gctgagtcgc gcccgctttg cggagtaggg gttagtaacc gcaacggctc
     4141 ttatgcccca gtgtttgttg atctggcaag taaagccaat gccagtccca caaatgtaaa
     4201 ttcccagacg gcactcatcg cgttgaataa tgcctgccat ttcgtcggcc agacgcgcga
     4261 agttattgac accttgttcc ttacatgtga cgggatcgta cacgatctcc atgtttttct
     4321 cttctatgag gtattttttt atctcctctt tacgtgcaaa acctacatca tcgcttgcga
     4381 tagcaatctt tgttttcatt tcattttgcc tgtcgttaag taac
//
LOCUS       DAOFQD010000226         4354 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.227, whole genome shotgun sequence.
ACCESSION   DAOFQD010000226 DAOFQD010000000
VERSION     DAOFQD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.227"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..68)
                     /locus_tag="QBF46_004662"
     CDS             complement(<1..68)
                     /locus_tag="QBF46_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676749.1"
                     /translation="MASISISCPSCSATEGVVRNGK"
     gene            126..3389
                     /gene="ypjA"
                     /locus_tag="QBF46_004663"
                     /pseudo
     CDS             126..3389
                     /gene="ypjA"
                     /locus_tag="QBF46_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
     gene            3375..3574
                     /locus_tag="QBF46_004664"
                     /pseudo
     CDS             3375..3574
                     /locus_tag="QBF46_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1224 a          968 c         1115 g         1047 t
ORIGIN      
        1 cttttaccgt tacgcaccac gccttcagta gctgagcagg agggacaact gatggagatg
       61 gaagccacgg gagcacctca aaaacaccat catacactaa atcagtaagt tggcaccatt
      121 accccggcgg tattgcacat ggggttaacc aggagacggg cagtgcttta gttgccaaca
      181 cgggtgcagg gactgatatc gaaggataca acaagctctc tcacttcact attaccggag
      241 gggaggctaa ttatgttgtg ctggaaaata ccggcgaact gacggtagtg gctaaaacct
      301 cggcgaaaaa tactaccatt gatactggcg gtaagctgat tgtccagaag gaggctaaaa
      361 cagatagcac cagacttaat aatggcggcg ttctggaggt tcaggacggt ggtgaggcta
      421 agcatgttga gcaacaatcc ggcggcgcat taattgcttc cacgacctcc ggaacactta
      481 tcgaaggaac caacagttat ggtgatgctt tctacatcag gaattcagaa gctaaaaatg
      541 tagtgctgga aaacgctggc tcattaacag tcgtcactgg ttcccgcgca gttgacacga
      601 ttattaatgc caacggcaaa atggatgttt atggaaaaga tgttggcact gtactcaata
      661 gtgctggcac ccaaacaata tatgccagtg ccacttctga taaagcaaat atcaaaggtg
      721 gcaagcaaac ggtatatggt ttagccactg aagcaaatat cgaaagtggt gaacaaattg
      781 ttgatggtgg gtcaacagag aaaacacaca tcaatggtgg cacgcaaacc gttcagaatt
      841 atggtaaggc aatcaatacc gatatcgtct ctggcctaca acaaattatg gcaaacggga
      901 cagcggaagg ttccattatt aatgggggtt cacaggtagt taatgagggc ggtctggctg
      961 aaaactcggt gcttaatgac ggcggcacac tcgatgtgcg ggagaaaggc agcgcaacgg
     1021 ggatacagca gagtagccag ggcgctttgg ttgcaaccac cagggcgacg cgggtcacag
     1081 gaacacgcgc ggatggcgtc gcgttcagca tcgagcaggg tgcggcgaac aatatcctgc
     1141 tggcaaatgg cggcgtgtta accgtggagt cagacacctc ttctgacaaa acacaggtca
     1201 atatgggcgg acgggagatc gtcaaaacaa aagccactgc gacaggcacg acgctcaccg
     1261 gcggtgaaca aattgtcgag ggtgtggcga atgagacaac aattaacgac ggcggaatac
     1321 aaacagtttc agctaacgga gaggcaataa aaacaaagat caatgaaggc ggtacgctga
     1381 cagtcaacga taatggcaaa gcgacagata tcgtccagaa cagcggtgcc gctctccaga
     1441 cgagcacggc taacggtatt gaaatcagcg gtactcacca gtacggtact ttttccattt
     1501 ccggcaattt agcgaccaat atgttgctgg aaaatggcgg taatttattg gtattagcag
     1561 gtaccgaagc tcgcgactcc acggttggca agggtggggc aatgcaaaac ctgggtcagg
     1621 actccgccac aaaggttaac tctggcgggc aatataccct tgggcggtca aaagatgagt
     1681 ttcaggctct ggcccgggca gaagatctcc aggtcgctgg cggtacggca atcgtctacg
     1741 caggtacgct ggcggatgca tcggtcagtg gcgcgacagg aagcctgtcg ttaatgacgc
     1801 cacgggataa tgttacgcca gttaaactcg aaggggcggt ccggattacc gatagcgcga
     1861 cattgactct gggaaatggc gtcgatacca cgcttgccga cctgacggct gccagccggg
     1921 gcagtgtctg gcttaacagc aataattcct gtgcaggtac cagcaactgc gaatatagag
     1981 taaacagttt gctactcaac gacggtgatg tttatttgtc agcacaaaca gcagcgcctg
     2041 ccacaactaa cggtatctac aatacgctga caaccaatga actttccggt agcggtaatt
     2101 tctacctgca taccaacgtt gcaggctccc ggggcgatca actggtcgtc aacaacaacg
     2161 ccactggtaa ttttaaaatc tttgttcagg ataccggcgt cagcccacag tctgacgacg
     2221 cgatgacgct ggtgaaaaca gggggagggg atgcttcgtt tacgctgggc aataccggcg
     2281 gtttcgttga tcttgggacc tatgagtatg tcctgaaaag tgacggcaac agcaactgga
     2341 acctgaccaa tgatgtcaaa cccaacccgg accccatccc aaatccaaag ccagacccaa
     2401 aacccgatcc aaagccagac ccaaatccaa aaccagaccc tactcccgat ccaacgccga
     2461 cacccgttcc ggagaaacgc attacgcctt ctacggcagc cgtactcaat atggcagcaa
     2521 cattaccgtt ggtatttgat gctgagctaa acagtattcg cgagcggttg aacataatga
     2581 aagcgagtcc acacaacaat aatgtctggg gggcgacgta taacacccgt aataatgtca
     2641 ccaccgatgc gggtgccggg tttgagcaga cgctgaccgg aatgacagtg gggatcgaca
     2701 gccgtaatga tattcctgaa ggaattacca cgctaggcgc ttttatgggc tattcccatt
     2761 cacatatcgg ttttgatcgc ggaggacatg gcagtgtggg cagttattct ctgggcggct
     2821 atgccagttg ggaacatgaa agtggtttct atctggacgg tgtcgtgaag ctgaaccgtt
     2881 ttaaaagtaa cgtagcaggt aaaatgagca gcggtggagc cgccaatggc agttaccaca
     2941 gcaacgggct gggcggtcac attgaaaccg ggatgcgatt taccgatggt aactggaacc
     3001 tgacgccgta tgcatcgtta acggggttca ccgctgataa ccccgaatat catttatcca
     3061 atggcatgaa atcgaaatca gtcgataccc gcagtatata tcgtgaactg ggcgcaacgc
     3121 tgagttacaa catgcgtctg gggaacggta tggaagttga gccgtggctg aaggcggctg
     3181 tgcgcaaaga atttgtcgat gataaccggg tgaaagtgaa tagtgacggt aatttcgtca
     3241 attatttgtc gggcagacgt ggaatatacc aggcaggtat taaagcctca ttcagcagta
     3301 cgttaagcgg gcatcttggg gtggggtata gccatagtgc cggtgtggaa tccccgtgga
     3361 acgcggtagc tggtgtgaac tggtcgttct ggccatcaac gaaaaagccc acatctgtgg
     3421 gctttcatgt taccaggagc cgcggctcct ttgcgtatcc ttttatgtct cctcaccgtc
     3481 tggtcggtgt tttgctgaga cttctaactt cctgtttttg ttggtgttgt ccttacaccg
     3541 tccaatcatg attggctggc gtccctgtaa ttagttattc aattcactga tttatggatt
     3601 tattttgatt tttaatttta tgcgtggttc taaaaaggaa ccataactgc ggcatagttc
     3661 ttcatatgag tgaccacaac agaaataagt tatatctaca ctatttttga aggcagaata
     3721 gagcaaggaa tcgacacatt ctttgtacca atttcaaaag gctgactcat agttgtagga
     3781 tagcgggagt ttatgcggat atttcagttt atatctaaaa ctagggggga aacccataag
     3841 caccatatgt tactattgta tttctaatac taaggattgc atctatagtc tatggttctg
     3901 ctgccgattg actaatcggg attgtttttg gtataggtta cttctatacg ggcttcccgt
     3961 tgcaaccagc tttgttagcc acatgagagt taaaagggaa aacttatggc tgatggtcca
     4021 aaagatttag accggttaga tttaatgtgc aaggtcatca ctagaacaga tggatttcac
     4081 aactacgcaa acacaaaatc gactgttata ttaacattca ccaccgcaat attagcctta
     4141 attattgcta atgtaagcca tttttatgaa tttttgaaca caagtaccgt tcctcatgtt
     4201 aagttagtat tctcgatatt actatttgct gaattgctgc tactattttt tgctttgttg
     4261 cttatatcaa agactctaat acctaatact gataaaagta aaacaaaaaa catttattct
     4321 tttgtggata tttatacaaa tttcaatgct gaag
//
LOCUS       DAOFQD010000227         4324 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.228, whole genome shotgun sequence.
ACCESSION   DAOFQD010000227 DAOFQD010000000
VERSION     DAOFQD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.228"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..222)
                     /locus_tag="QBF46_004665"
     CDS             complement(<1..222)
                     /locus_tag="QBF46_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676750.1"
                     /translation="MLVFLYNSTKGKTMRLYIIGNGFDMRHGLPTGYKHFKSYVEKND
                     QDLYDAIEEYIPAGDEWNELENALGEIDYE"
     gene            875..2215
                     /locus_tag="QBF46_004666"
     CDS             875..2215
                     /locus_tag="QBF46_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDQ6676751.1"
                     /translation="MIKETVTMSHKELHRLQIIQESINRHITQEQAAARIGISIRQVK
                     RLVQRYRNEGPSGLVSHRRGKRPNNSFSTEFRATVISLLKGRYADFGPTFACEKLREI
                     HGLSLSVETLRKWMIEEGLWRERRRKIARIYQRRMRRPSYGELIQIDGSPHDWFENRG
                     PRCTLIVFIDDATSALMALRFVPAETTRAYMETLRGYLDDHGVPLALYSDRHSIFRVN
                     NPEREGELTQFTRAIKTLGIEPIHANSPQAKGRVERANQTLQDRLVKEMRLQNISDIE
                     TANAWLPTFIEAYNNRFATSPRTTDNAHLDVHHSEEELGYIFSLQAKRVLSKNLTFQY
                     KSSAFQVRSEGRGYRLRHSVVTVCENFDGEINVLYDGKALGWEKYVDGPEPIPLDDEK
                     SVHERVDNARIDLRSKYYVKPKADHPWLTRRTQSHQQVKPPKLPKKKPDPDKID"
     gene            2496..3494
                     /locus_tag="QBF46_004667"
     CDS             2496..3494
                     /locus_tag="QBF46_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDQ6676752.1"
                     /translation="MALTVTAAFNEFQRNIVNLDSSQTDRARASRDWLLGRMNTFPNN
                     DIYFPIIYPDIHTGFGSFARRTKIRPLDDIDLMFGLDGDDCVYIESDGKILITAREGT
                     VRLKYYKHDGTNFINSRKIINSFISSLNQIPQYDKADVKRNQEAATLKLKSYDWNFDI
                     VPAFITTPDSFGKTYYLIPDGNGHWKKTDPRIDKQKVTDLNVKLSGNMLNVIRVIKYW
                     QKRPTMPSMSSYLLETMLLNYFNGRPECYQWVDLEIVNVLGYLASAIFSPVYDHKGIQ
                     GNINNLSDDDKLKISIRCYLDQGKATEARNYESQDNHRASINKWQDVFGVNFPSYG"
     gene            3498..>4324
                     /locus_tag="QBF46_004668"
     CDS             3498..>4324
                     /locus_tag="QBF46_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676753.1"
                     /translation="MSQIFLRQNDSKNIELLMAMRRTYSQVKFVNWVVFFLSVVISVA
                     IVIFTTVIKKMGLFPHLAIAPYLGYYGIIVLVITIIFSTVISSMKKCAATIQEMFDCD
                     VLHIPWSELKVGKRVCREDIFKASKYYKKRNKKDEFLNWYLNKDYEANENVMALLCHA
                     KNFGWDKSQRDVMSKIYFITMLLSFLILLAYGLWSKSSLEDFLFYIVFTLPFFRHVIM
                     LYVENNKSINRIIRVKDFIEKKIQSIKISGMINDDILSHELRAIQDEVYAHRSTSNPV
                     "
BASE COUNT         1286 a          843 c          933 g         1262 t
ORIGIN      
        1 ctcataatca atttcaccta aagcattttc taactcattc cactcgtccc cagcaggaat
       61 gtattcttct atcgcgtcat atagatcttg gtcatttttt tctacatacg acttaaagtg
      121 cttgtatcct gttggtaagc catgacgcat gtcaaatcca tttcctatta tgtaaagcct
      181 cattgttttt cccttagtcg aattgtataa aaatactaac atggcctgct ccacgttgac
      241 tactatcccc tcctttgagt aatgccttca tcagatgtaa caacatttgc gagcttccac
      301 agttcgctca aagcggacta gaaggttagc ttgcgtcgga cttggcgtat ttaaagaagt
      361 gctggtggag actggttgtt gtgttccatt tctacagaac aaaatcacag aaactatacc
      421 caatagttat attgaatcaa tgatgagaca gcctcatatt tatcaggact ggtgtacgtc
      481 caatacagga ggttgtcgtg ctggttctca aatgtgcgct agctattgcg gctgtaatgg
      541 caatttattg tcttgctgtt gttcttacgg atcgcctttc tgattgattt tatattggcg
      601 aggtgacgtg agttaagtag aattgctgcg ggtgcttgag gctatctgcc tcaggcatga
      661 acaccaaagg cagatagaga aaagccccag ttaacattac gcgtcctgca agacgcttaa
      721 cattaatctg aggccaattt catgctagac acatgtaggt tagcctctta cgtgccgaaa
      781 ggcaaggaga agcaggctag tgttaacacc aagctgtaat gtcccctttg aaccattcta
      841 aaatgtcccc agacaattct ctgggggatt tttcatgatc aaagagactg ttacgatgag
      901 tcataaggaa ctccaccgac ttcagattat tcaggagtca attaatcgac atattactca
      961 ggaacaagct gcggcacgca ttggcatttc tattcggcag gttaaacgtc tggtgcaacg
     1021 gtatagaaat gaagggcctt ctggtctggt ttcccaccga cgtggaaagc gtcctaataa
     1081 ttccttttct actgaattca gagcaacagt aatttcactc ctcaaaggcc gttacgctga
     1141 ttttggacct acgtttgcgt gcgaaaaatt gcgcgagata cacggtttat ctttatccgt
     1201 tgaaactctc agaaagtgga tgatagaaga ggggttatgg cgtgaacgcc gtcgtaaaat
     1261 tgcccgtata tatcaacgcc gcatgcgacg accatcttac ggtgaactga tccagattga
     1321 tggctcacct catgactggt ttgaaaatcg aggccccaga tgtacactga tcgttttcat
     1381 tgatgatgcc accagtgcgt tgatggcgtt gcgttttgtg cctgctgaaa caacccgggc
     1441 ttacatggaa accctccggg gttaccttga tgatcatggc gtaccgctcg ctctctactc
     1501 tgatagacac agtatattca gggtaaataa cccagagcgg gaaggtgagc tgacccagtt
     1561 cactcgtgcg ataaagacac tgggcatcga gccaatccat gccaacagcc cgcaggcaaa
     1621 agggcgggta gagcgcgcca atcagacact acaggacagg ctggttaaag aaatgcggct
     1681 tcagaatatc agtgatattg aaacagcaaa tgcatggttg ccgaccttta ttgaagccta
     1741 taacaaccgg ttcgctacgt cgcctcgtac tactgataat gctcatcttg atgtgcacca
     1801 ttctgaagag gaactgggtt atatcttcag cctacaggcg aagcgcgttc tgtctaaaaa
     1861 tctcactttc cagtacaaaa gcagtgcgtt tcaggtacgc agtgagggcc ggggatatcg
     1921 acttaggcat tcggttgtta ctgtatgcga gaactttgac ggtgaaatta acgttctgta
     1981 tgacgggaaa gcgctgggct gggaaaagta tgttgatggc ccggagccta taccactgga
     2041 tgatgaaaag agtgtccatg aacgagtgga taatgcccgt attgatttac gttcaaaata
     2101 ctatgttaaa cctaaagctg accatccctg gcttacgcgc cgaacgcaaa gtcatcagca
     2161 agttaagccc ccgaagttac ctaaaaagaa gcctgacccc gataaaatag attgaaacca
     2221 agatcgattc ggttgagtgc atatccattc atagggtaga ttcttaagtc gcgtttctgg
     2281 tgttcatttt cggggggggt tgttacttgt tttaccgggg atatgccaga aacgcgctga
     2341 gtcagtctgg gctgtcaagg ctaagttcag atgtccgggg tagggcaaag ttcaaatgtc
     2401 ctccaatact cagtagtttg caggagtaaa ttcatgtgga gacagcctat aacctaatgg
     2461 taaaattagc ctttagctgc tagaagaggg atgctatggc tcttacggtg actgctgcgt
     2521 ttaacgagtt tcagcgaaat atagtaaatc tagatagctc tcagactgat cgagccagag
     2581 ccagtagaga ttggttgttg ggcagaatga acacttttcc aaataatgat atttatttcc
     2641 ccataatata ccctgatata catacaggtt ttggttcttt tgctagacgc acaaaaatcc
     2701 gccctttaga tgatatagat cttatgtttg gtctcgatgg tgatgattgt gtatacatcg
     2761 agagtgatgg gaaaatatta atcactgcgc gtgaaggaac agttcgctta aaatactata
     2821 agcatgatgg cacaaatttt attaactcta gaaaaataat aaacagtttt atctcaagtc
     2881 taaatcaaat tccgcaatat gataaggcgg atgtaaaaag aaatcaggaa gcagctactc
     2941 tgaagctaaa atcctatgac tggaattttg atatagttcc tgcattcatt actactccag
     3001 actcatttgg aaagacatat tatttaatac cagatggtaa tggtcattgg aagaagacag
     3061 atccacgaat tgacaagcaa aaagtcacag atcttaatgt taaactctca ggaaacatgt
     3121 taaatgtcat tcgtgtaata aaatattggc aaaaacgtcc tactatgcct tcaatgagta
     3181 gttacttgtt agagacaatg ttacttaact acttcaacgg tcgcccagaa tgttatcaat
     3241 gggtcgattt agagattgtc aatgttttag gttacttagc ctctgctata tttagtccag
     3301 tatacgatca caaaggcata caagggaata taaacaacct tagcgatgat gataaattaa
     3361 agatatccat ccgctgttat cttgaccaag gaaaagcaac ggaagcccgt aattacgaaa
     3421 gccaagataa ccacagagcc tccattaata aatggcagga tgtttttggt gtcaacttcc
     3481 catcatacgg atgaaaaatg agtcaaatat ttttgcgtca aaatgatagt aaaaacattg
     3541 agttactaat ggcaatgagg cggacttact cacaggttaa atttgttaac tgggtagtgt
     3601 tttttcttag tgtcgtaata tctgtcgcta tagtaatatt tactacagtg ataaagaaaa
     3661 tggggctgtt ccctcatttg gctatagctc cttacctcgg ttattacggt ataattgtat
     3721 tggtcatcac aattattttt tccacagtga tttcctcaat gaaaaaatgt gcagccacta
     3781 ttcaggaaat gtttgactgt gatgttttac atattccatg gagtgaatta aaagtaggta
     3841 agcgggtttg tagagaagat atttttaagg cttcaaagta ttacaagaaa agaaacaaaa
     3901 aggatgagtt tcttaactgg tatctaaata aagattacga agccaatgaa aacgtgatgg
     3961 cattattatg ccatgcgaaa aatttcggct gggataagtc acaacgtgat gttatgagta
     4021 aaatctactt tatcactatg ttactttcct ttttgatact tcttgcatat ggtttgtggt
     4081 ctaaatcttc actggaagat tttttatttt atattgtttt cacgcttcct ttctttagac
     4141 atgtgataat gttatacgtt gaaaacaaca aatcaatcaa tcgaattatt cgcgtcaaag
     4201 attttattga gaaaaaaata cagagcataa agataagtgg catgataaac gatgatatct
     4261 tatctcatga attaagagcg attcaggatg aggtttatgc ccatagatcg acctctaatc
     4321 ctgt
//
LOCUS       DAOFQD010000228         4223 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.230, whole genome shotgun sequence.
ACCESSION   DAOFQD010000228 DAOFQD010000000
VERSION     DAOFQD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.230"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            310..1203
                     /locus_tag="QBF46_004669"
     CDS             310..1203
                     /locus_tag="QBF46_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDQ6676754.1"
                     /translation="MKSRYITPVVTIFDEQGRIDPEQNTQIYDFIKNHVSGFVVMGST
                     GEFFSLNMENARQMIKTCSEFSRGDMKVYAGASRMDVNESISLANYAWEQGLDGVMII
                     SPYYFPLDDEAIFDFYSTIASQTPAHIFIYNFPERTGYSVSPEICLRLADKYPNIIGM
                     KDTVPDAFHTAQVINTVKKNIPHFEVYAGYDNNFAHNILSGGDGCIGGLSNIVPEFFA
                     SWMQAFTNDDLLAVAQHQQIVDQLMAVYSVNSPFIPTFKKALQLKGVIQSERCSSPFN
                     ILDDIQSRRLQEILSVTDYYK"
     gene            1254..2555
                     /locus_tag="QBF46_004670"
     CDS             1254..2555
                     /locus_tag="QBF46_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6676755.1"
                     /translation="MANITQYDNPLLNSAIQKSWKRLVPLMFIMYFVAFIDRVNVGFA
                     KDAMKLDIGLSESAFALGAGIFFAAYALFGIPANLILNKIGAQKWLSITTAIWGLLSA
                     MTGFVTSETQFIILRFLLGLGEAGFYPGILLLASIYFPNKVRGSVVGIFVLGVPLALT
                     LGSPISGALLELHGWMGRPGWFWMFVIEGLPALVLGIFAWFYLDDNPTKARFLTQEEK
                     DALNKQLESERQKTETSSVISALKNAKVWHLALIYGTIQISVYGLMFFLPSQVASLMG
                     ESLGFKQSLVAAIPWACSAFGVYYIPRIADRNPSRRVAISVMCMLAAAVGLALSAFAG
                     PVVAIGALCLSAVGFLSVQPIFWTFPPQLLTGPALAAGIGFCTTMGAFCSFLAPLIRV
                     EVDRAMDSSIAGLMALSVITIGCALLIGLLQKSQQAAKAYS"
     gene            2589..4184
                     /locus_tag="QBF46_004671"
     CDS             2589..4184
                     /locus_tag="QBF46_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDQ6676756.1"
                     /translation="MQKILRIDSNDNLIVALKDLHAGESFSWDDDNITLVTDVKAKHK
                     FATQDIPLDGIVSMYGTPVGKATRPIVKGEAITVDNIRHYAAPVTLEDVEPYHWQAPD
                     VSEWSTRTFKGYVRDDGRVGTASYWLVFPLVFCENRNVSTLTNALNDALGYTNNSLKK
                     FALNLTSGSDELIETARMFPHIEGVRCITVTSGCGGATSDCETMCDVLAAYADHPNVI
                     GMTVFSLGCEKAQQKMFKDALARRNPEFDKPALYFLQQEWDSEERMMQTALQQTFEAM
                     KAVKPTERVEVPLSCLKVGMKCGGSDGFSGISGNPAMGLVSDWLTMLGGASGLAEFPE
                     LCGAEGDMVKRCINLEDKKKFLNLMQGYEKTANFFDTTIADNPSFGNIADGLITDAIK
                     STGAAKKGGRAPIVSVCDYAEPMPDSGLSLVCTPGNDVDAVTGLVAAGCNVVIFSTGL
                     GTPTGNPIVPVLKISTNSTIARRLSDMIDFDCGPVIDGTPLEDVSRELFEKVIETASK
                     DYVVKADKLEQFDFMLWKRSLDL"
BASE COUNT         1109 a          848 c         1013 g         1253 t
ORIGIN      
        1 atgagtaatg caccattcta gggagaatta taattcaagt aaattaaccc cctcgcgttc
       61 cttgactgct gttgagccca tcagtccttt tttagcgtga tactggtcat aaaacggata
      121 aaaaaagaac tgcgattgtt ttttatgcag atctctgaca taattactcc aaatatatca
      181 atagcttatc tgtatcactt ctgtgattgg ctaaatttga ctcatttcta aggttgggta
      241 tactcatttt agtttgtatt taaaaacgtt gtttgtatat atgaacaata aaactatgat
      301 gaggtaggca tgaaaagtcg ttacataacc cccgttgtca ccatatttga tgaacaaggg
      361 cgaatcgatc ctgagcaaaa cactcagatt tatgatttta ttaaaaatca cgtgtccggg
      421 tttgttgtga tgggcagtac tggtgagttt ttttctctta atatggaaaa cgcccgccaa
      481 atgattaaaa cctgtagtga gttttcccga ggcgatatga aagtttatgc cggagccagt
      541 cgcatggacg tcaatgaatc aatatcactg gccaattacg cctgggagca aggattggat
      601 ggtgtgatga tcatcagtcc ttattatttc cctctggatg atgaagctat ttttgatttc
      661 tacagtacga ttgcaagcca aaccccggct catattttta tttataactt cccggagaga
      721 accgggtaca gcgtatcacc agagatttgt ttacgactgg cagataaata tcccaatatt
      781 attggtatga aagataccgt ccctgatgct ttccatactg cgcaggttat caataccgta
      841 aagaaaaata tccctcattt tgaagtgtat gcaggatatg acaataattt tgcccacaac
      901 atactatctg gtggagatgg ctgtattggt ggtttatcca atattgtccc tgaatttttt
      961 gcctcctgga tgcaagcatt cacgaatgac gatttacttg cagtcgcgca gcatcagcag
     1021 atagtcgatc aactaatggc cgtttacagc gtgaattctc cttttattcc cactttcaaa
     1081 aaagcccttc agttaaaggg ggttattcaa tcagagcggt gttcttcacc atttaatata
     1141 cttgatgata ttcaatcccg gagattgcag gaaattttat ctgtcaccga ttattataaa
     1201 taatataaaa cctataaata gaatatctta ccaacagaac taaggattta tttatggcta
     1261 atataaccca atacgataat ccgctgttga attccgcaat tcaaaaatcg tggaaacgtt
     1321 tagtaccctt aatgttcata atgtattttg ttgcctttat tgatcgggta aatgttggct
     1381 ttgccaaaga tgcaatgaaa ctggatattg gattatcaga gtcggcattc gctctcggtg
     1441 caggaatatt tttcgctgcc tatgcacttt tcggcattcc agctaacctg atcctcaata
     1501 aaatcggcgc acaaaagtgg ttaagtatta caactgcaat ttggggcctt ctctcggcaa
     1561 tgacgggatt tgtcaccagt gaaacacaat ttattatttt gcgctttctg ttagggctgg
     1621 gcgaggcggg gttctatccc ggaattttac tgctcgcatc catttatttt cctaacaagg
     1681 ttcgcgggtc ggtcgtggga atatttgtgt tgggggtgcc gctggcgtta acgctcggtt
     1741 cgccaatatc gggagcttta cttgaactac atggctggat gggacgtcca ggatggttct
     1801 ggatgtttgt cattgaggga ttgccggcat tagtgcttgg tatctttgcc tggttctacc
     1861 ttgatgataa tccgacgaaa gcgcgatttc ttacccagga agaaaaagac gctttgaata
     1921 agcaactgga atctgaacga cagaaaacag aaaccagttc agtgatttca gcattgaaaa
     1981 atgccaaagt ttggcatctg gcattaatct acggcaccat ccagatatca gtatatggtt
     2041 taatgttttt ccttccgtca caggttgcat cgctgatggg agaaagcctt ggctttaagc
     2101 agtcattagt cgcggcaata ccatgggcgt gctctgcttt tggtgtctat tacattccgc
     2161 gaattgctga ccgcaatcct tcccggcgtg tggccatttc ggttatgtgt atgttggctg
     2221 ctgctgtcgg ccttgccctt tccgcatttg cggggcctgt tgtggcgatt ggtgcacttt
     2281 gtttaagtgc ggttggtttc ctctcagtac agccaatatt ctggactttc cctccacaac
     2341 ttcttactgg tccggcactg gctgcgggga tcggtttttg caccacgatg ggcgctttct
     2401 gctcgttcct tgctcctctt atccgtgttg aggttgatcg ggcaatggat agcagcatcg
     2461 ccggattgat ggcgttgtct gtaattacta tcggctgcgc attattgatt ggattgctgc
     2521 aaaagagcca acaggcagca aaagcataca gttaataaga ttgaacatct gactggaaga
     2581 atatgattat gcaaaaaata ttacgtatcg acagtaacga taatctcatt gtcgcgttga
     2641 aagacctgca cgctggagaa tcgttctcgt gggatgacga caacataacg ttagtaactg
     2701 atgttaaggc taaacataag tttgcgacgc aagatattcc tctggatggc attgtttcga
     2761 tgtacggtac gccagttggc aaggccacgc gtcccatcgt taagggagaa gcgattaccg
     2821 ttgataatat tcgccactat gcagctcctg tcacactgga ggatgttgaa ccctaccact
     2881 ggcaggcacc ggatgtctct gaatggagca cccgcacttt taaaggttat gtccgtgacg
     2941 atggcagagt gggtactgcc agttactggt tagtctttcc attggtattt tgcgagaacc
     3001 gtaatgtcag tacgttaacc aatgctttga atgatgcttt gggttatacc aataactcac
     3061 tgaaaaagtt tgctcttaat ctgacgtccg gtagcgatga actgatcgaa accgcgagaa
     3121 tgtttccgca tattgaaggt gttcgctgta ttacggtcac cagtggttgt ggtggtgcaa
     3181 cttcagactg tgaaactatg tgcgatgtat tagctgctta tgctgaccat ccgaatgtaa
     3241 tcggtatgac ggtattcagt ttaggatgtg aaaaagcgca gcagaaaatg tttaaggatg
     3301 cgctggcgag acgtaatcct gaatttgata aacctgcgct ctactttctc caacaggagt
     3361 gggacagcga agagcggatg atgcagaccg ctttacagca gactttcgag gcaatgaaag
     3421 cggtgaagcc gacagaaaga gtagaggtcc ctctttcctg tctcaaagtg gggatgaagt
     3481 gtggtggttc cgatggtttt tctggtatct ccggcaaccc tgcaatgggg ctggtttctg
     3541 actggttgac aatgcttggt ggcgcttccg ggttagcaga atttccagaa ttatgtgggg
     3601 cagaaggtga tatggttaag cgttgtatta atcttgagga taaaaaaaag ttcctcaatc
     3661 taatgcaagg ctacgaaaag acagcgaact ttttcgatac caccattgcg gataacccaa
     3721 gttttggcaa catcgcggat gggttgatta ctgacgcaat aaaatctacc ggagcagcga
     3781 aaaaaggcgg gcgagcgcca attgtgagtg tatgcgatta cgctgaacct atgcccgata
     3841 gtggattatc gttggtgtgt actccgggca atgacgtgga cgcggtaacc ggattggtag
     3901 cagcaggatg taatgtggtt atcttctcga cgggtcttgg tacgcccacc ggtaacccga
     3961 ttgttccggt gttaaaaata tccaccaaca gcacgattgc cagacgattg tctgacatga
     4021 ttgatttcga ctgtggccca gtgattgacg gcactccgct ggaagatgtg agtagagaat
     4081 tatttgaaaa agttattgaa actgccagta aggactacgt cgtgaaggca gacaaactcg
     4141 aacagtttga ttttatgttg tggaagcggt cgttagactt gtagtgacta atcattagcg
     4201 tcagtgtatg tagactggcg cat
//
LOCUS       DAOFQD010000229         4221 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.231, whole genome shotgun sequence.
ACCESSION   DAOFQD010000229 DAOFQD010000000
VERSION     DAOFQD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.231"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            354..962
                     /locus_tag="QBF46_004672"
     CDS             354..962
                     /locus_tag="QBF46_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDQ6676757.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1060..2451
                     /gene="selA"
                     /locus_tag="QBF46_004673"
     CDS             1060..2451
                     /gene="selA"
                     /locus_tag="QBF46_004673"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDQ6676758.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2448..>4221
                     /gene="selB"
                     /locus_tag="QBF46_004674"
     CDS             2448..>4221
                     /gene="selB"
                     /locus_tag="QBF46_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDQ6676759.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFD"
BASE COUNT          944 a         1085 c         1240 g          952 t
ORIGIN      
        1 ccgccggggc acaccgaaca ggccacgccg gtgggggcac caatgcgcac cccggctgaa
       61 ccctgaacaa tgctaccgcc atactgcgtc atgtcgccct gacgcgctgc cggttttccg
      121 ctcatctgct catccttgtt ttatcgggtt atttgtctgc ttccaggcaa aaccctacag
      181 atttataaga aagccttaag tgagatatcc atcagataga aacaattagg aaaatattta
      241 tatcaccatg ataaatatgt gatttatttg gaagtaacaa accatcaaca ttttctgccc
      301 tttttcccat caccaggcca tactgatatt tttcctcgca caaaggactc ggcatgaaac
      361 tcgtcggtag ctacaccagc ccgtttgtac gcaaactttc tattctgttg ttagaaaagg
      421 gcataacttt cgaatttatt aatgaactgc cctataacgc ggacaacggc gtggcgcaat
      481 ttaacccgtt aggaaaagtg ccggtgctgg tgaccgaaga gggcgaatgt tggtttgatt
      541 cgccgatcat cgctgaatat attgaattaa tgaatgtcgc tccggcgatg ttgccgcgcg
      601 atccgctgga gtcgttgcgg gtgcgcaaaa ttgaggcact ggcggatggc attatggatg
      661 ccgggctggt atcggtgcgt gaacaggcgc gtccagcggc gcagcagtct gaagatgaat
      721 tgttacgcca gcgggagaaa atcaaccgca gtctggatgt gctggaagga tatctggtcg
      781 atggcacact caaaaccgat acggtcaatc tggcgactat cgccattgcc tgtgccgtcg
      841 gatatctcaa tttccgccgc gttgcgccag gctggtgtgt cgatcgccca cacttagtca
      901 aactggtcga aaacctgttt agccgcgaaa gttttgctcg caccgaaccg ccaaaggctt
      961 gatgcgcgat atgtcctcct gacccatctc acgttacaat ccgtggttat gttaaacgcc
     1021 cttctccgtg tgagagggcc ttgatcagcc aggtttccta tgacaaccga aacgcgttcc
     1081 ctctatagtc aacttccggc tattgatcgc ttattgcgcg atagctcctt cctttctttg
     1141 cgtgatactt atggtcacac ccgcgtggtg gaattgttgc gtcagatgct cgacgaagcg
     1201 cgagaagtga ttcgtggcag ccagacgctg cctgcgtggt gtgaaaactg ggcgcaagaa
     1261 gtcgatgccc ggttgacgaa agaagcgcag agcgcgctgc gtccggtgat caacctgacg
     1321 ggaaccgtgc tgcataccaa ccttgggcga gctttacagg cggaagccgc ggtggaagcc
     1381 gttgcgcagg ctatgcgttc gccagtgacc ctcgagtacg atctggacga cgctggacgc
     1441 ggacatcgcg atcgtgcgct ggcgcagttg ctgtgccgca ttacgggggc ggaagatgcc
     1501 tgtatcgtta ataacaatgc ggcggcggtg ttattgatgt tggcggccac tgccagcggg
     1561 aaagaggtgg tggtttctcg cggcgaactg gtggagattg gcggcgcgtt tcgtattccc
     1621 gatgttatgc gtcaggcagg ctgcacccta cacgaagtag ggaccaccaa ccgcacgcac
     1681 gcgaatgatt attgtcaggc ggtgaatgaa aataccgcac tgttgatgaa agtacatacc
     1741 agtaactaca gcattcaggg gttcaccaaa gcgatagatg aagcggaact ggtggcgctc
     1801 ggcaaagagc tggatgttcc cgtagtgact gatttaggca gtggctcgct ggtcgatctt
     1861 agccagtacg gtttgccgaa agagccaatg ccgcaggagt tgattgcggc gggcgtcagt
     1921 ctggtgagtt tctccggcga caagttgtta ggcgggccgc aggcaggaat tattgttggt
     1981 aaaaaagaga tgatcgcccg cctgcaaagc cacccgctga agcgtgcatt acgcgcggat
     2041 aaaatgaccc tcgcggcgct ggaagccacg ttgcgtcttt atttacaccc tgaagctctg
     2101 agtgaaaaat taccgaccct gcgcctgctt acccgcagcg cagaggtcat tcaaatccag
     2161 gcacaacgtt tacaggcccc ccttgccgca cattacggcg cggagtttgc ggtacaggtt
     2221 atgccatgtc tttcgcagat tggcagtggt tcgctgccgg ttgatcgcct gccgagcgcg
     2281 gcattaacgt ttacacccca tgatggacgc ggtagccacc ttgagtcatt agccgcccgc
     2341 tggcgtgaat tgccagtgcc ggtgattggt cgtatttatg acggacgatt gtggctggat
     2401 ttacgctgcc ttgaagatga gcaacggttt ttggagatgt tgttgaaatg attattgcga
     2461 ctgccggaca cgttgaccac ggcaaaacaa ccttattgca ggcgattact ggcgtaaatg
     2521 ctgaccgtct gccggaagaa aaaaagcgcg gcatgaccat cgatctcggc tatgcctact
     2581 ggccgcagcc ggatggtcgc gtgcctggtt ttatcgacgt tcccggtcat gaaaaatttc
     2641 tttccaacat gctggcgggc gttggtggta tcgatcacgc gctgttggtg gtggcgtgcg
     2701 atgacggtgt gatggcacag acccgtgagc atctggcgat tttgcagctg accggtaacc
     2761 cgatgctgac agtggtgctg accaaagccg atcgcgtgga cgaagcgcgt gttgatgagg
     2821 ttgaacgtca ggtaaaggag gtgctgcggg aatacggttt tgctgaggca aaactgttta
     2881 tcaccgcagc aaccgaaggt cgaggaattg atgccctgcg cgagcatctg cttcagttac
     2941 cagaacgtga acacgccagc caacatagtt tccgcctcgc gatcgaccgc gcatttaccg
     3001 taaaaggtgc cgggctggtc gtcaccggta cggcgttaag cggggaagtg aaggtaggcg
     3061 attcactatg gctaactggt gtaaataaac cgatgcgtgt acgtgcgctg catgcacaaa
     3121 accagccaac agaaaccgcc catgccgggc agcgtatcgc gcttaacatc gcgggtgatg
     3181 cggaaaaaga gcagattaac cgtggcgact ggctgcttgc cgatgcgccg ccagagccgt
     3241 tcacacgggt gattgtcgag cttcaaaccc atacgccgct gacccagtgg cagccgctgc
     3301 atattcacca cgccgccagc cacgttacgg gacgcgtttc gctgctggaa gataaccttg
     3361 ccgagctggt gttcgacacg ccgctgtggc tggcggataa cgatcgctta gtgctgcgcg
     3421 atatctcggc ccgcaacacg ctggcggggg cgcgtgtggt gatgcttaac ccgccgcgtc
     3481 gcggtaaacg taagccggaa tatctgcaat ggctggcgtc tcttgcacgg gcgcagagcg
     3541 atgccgatgc gttatctgtt catctggaac gcggcgcggt taaccttgcg gatttcgcct
     3601 gggcgcgcca gctcaacggc gaagggatgc gcgaattgct gcaacagcct ggttatattc
     3661 aggctggtta tagcttgttg aatgcgccgg ttgccgcccg ctggcagcgg aaaattctcg
     3721 acacattagc gacttatcat gagcaacatc gcgatgaacc tggtcctggg cgcgaacgtc
     3781 tgcgacgtat ggcgttgcca atggaagatg aagcgctggt actgttgctg attgaaaaga
     3841 tgcgcgaaag cggcgacatc ctcagtcatc acggttggct gcatctgcca gatcacaaag
     3901 cgggcttcag cgaagagcag caggccatct ggcaaaaagc agagccgctg tttggtgacg
     3961 agccgtggtg ggtgcgtgac ctggcaaaag agacgggaac cgacgagcag gcaatgcgcc
     4021 tgacgctgcg ccaggcggcg cagcaaggaa taattaccgc gatcgtcaaa gatcgttatt
     4081 accgtaacga tcggattgtc gaatttgcca atatgatccg cgatctcgat caggagtgtg
     4141 gttcaacctg cgcggcggat ttccgcgatc gcttaggcgt aggccgaaag ctggcaattc
     4201 agattctgga atattttgac c
//
LOCUS       DAOFQD010000230         4154 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.232, whole genome shotgun sequence.
ACCESSION   DAOFQD010000230 DAOFQD010000000
VERSION     DAOFQD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.232"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            80..583
                     /locus_tag="QBF46_004675"
     CDS             80..583
                     /locus_tag="QBF46_004675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676760.1"
                     /translation="MSLIKDIIERARENIRKVVSGAYTYKNEYLKLEDSFYQIISSII
                     SEGLDWNDDKVVSLFERIIEKKRKLTRNDGAWEQYEQLAKWLIYFGSIIEVSNTKYEK
                     TYLDAVEYSMGHMSKERNWGVSWYAYRAWETRWPAIIASNRLMIKNHIETLGTLKDAN
                     LIVKNIV"
     gene            complement(630..878)
                     /locus_tag="QBF46_004676"
     CDS             complement(630..878)
                     /locus_tag="QBF46_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modulator protein"
                     /protein_id="HDQ6676761.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGK
                     WRTVVDFKTNSVPQGVRDRAFEEWEQIIIDNALLLNAD"
     gene            complement(875..1315)
                     /locus_tag="QBF46_004677"
     CDS             complement(875..1315)
                     /locus_tag="QBF46_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDQ6676762.1"
                     /translation="MATLSDTIKPNKTYLEAVLRTALLGKTEDEYVDFFLSGLRGRLL
                     KNPRLYRSYGPYWPEIKKLLLERGYGNFGRLVDRDVRKIYRYDRPALTLIAATLYSQE
                     RFDNGQIYSAWHLLPVPEEVDDQDYEFESYDLEVEALAQAGEKT"
     gene            complement(1349..1870)
                     /locus_tag="QBF46_004678"
                     /pseudo
     CDS             complement(1349..1870)
                     /locus_tag="QBF46_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="darB domain protein"
     gene            2125..2472
                     /gene="tnpB"
                     /locus_tag="QBF46_004679"
     CDS             2125..2472
                     /gene="tnpB"
                     /locus_tag="QBF46_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDQ6676763.1"
                     /translation="MINLPAGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLMEGIDW
                     RQPKRLLTSLTML"
     gene            2522..4057
                     /locus_tag="QBF46_004680"
     CDS             2522..4057
                     /locus_tag="QBF46_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDQ6676764.1"
                     /translation="MNDTSSDDILLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNLLQQESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPTEPCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACRRC
                     DRIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYARQGVVLSRSVLSG
                     WVDACCRLLAPLDEALQHYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGHIKEAACWAHA
                     RRKIHDVHVRTPSVLTEEALKRIGELYAIESEIRGKRAEERQAVRHQKVLPLLASLEG
                     WLREKQKTLSRHSELAKAFGYALNQWPALIRYAEDGWVEVDNNIAENALRRVSLGRKN
                     WLFFGSDHGGERGASLYSLIGTCKLNGVDPERYLHYVLDVIADWPVNRVGALLPWRVT
                     LPA"
BASE COUNT          991 a         1065 c         1107 g          991 t
ORIGIN      
        1 ttattacttc tattgctttg gtataagggt aattgacaat acagaaatag attgtttctt
       61 tagtcgatta agttataaga tgtctctcat taaagacatc attgaaagag cgcgtgaaaa
      121 tatcagaaaa gttgtttcgg gggcttatac ttataaaaat gaatatctta aattagagga
      181 ttcattttat caaattatat catcaattat atcggaaggt ttagattgga atgatgataa
      241 agtcgtttct cttttcgaaa ggataataga aaaaaagcga aagttaacaa ggaatgacgg
      301 agcgtgggag caatatgaac agttggcaaa atggcttata tattttggaa gcatcataga
      361 ggttagcaat actaagtatg aaaaaacata tttagacgca gttgaatact cgatgggtca
      421 tatgagtaaa gaaaggaatt ggggagtttc ttggtatgct tacagagctt gggaaacacg
      481 atggcctgca ataattgcct caaatagatt aatgataaaa aaccacattg aaacattagg
      541 caccttgaag gacgccaacc ttatagttaa gaatatagtg taattgtggt cgataaaaat
      601 ttatcgggca gataaaactg cccgataaat taatcagcat tcaggagcaa tgcgttatct
      661 atgatgattt gctcccattc ttcgaatgcc cggtcgcgga cgccctgggg aacgctgtta
      721 gttttgaaat cgacgaccgt acgccatttc ccatccggac ggtacatacg cagagcttta
      781 cttccccctt ccctgcgcac ctcaacgtta tgcttgtcag caaactcttg taatgctcgt
      841 agcgtcccat gctttactgt gtagtatcgc tttttcaagt tttctctcca gcctgtgcca
      901 aggcttcaac ttccaaatcg taagactcaa actcatagtc ctggtcgtca acttcttcag
      961 gcactggcag taaatgccag gctgagtata tctgaccatt atcaaaacgc tcctggctgt
     1021 agagcgtcgc ggctatgagt gttagcgccg ggcggtcata acggtaaatt ttgcgaacgt
     1081 cacggtcaac gagacgaccg aaattaccat aaccgcgctc cagtaataat tttttaattt
     1141 ccggccagta tggaccatag ctgcggtaca ggcggggatt tttcagtaat cgcccgcgta
     1201 gccctgacag gaagaaatca acgtattcgt cttctgtctt tcctaacaac gctgtacgga
     1261 gtaccgcctc aagatatgtt ttattcggtt ttattgtatc agatagtgtg gccatattat
     1321 gcgacgcccg gcgaaccggg cgctcctgtt atgcgtattg ttggatgacg gccagaacgt
     1381 ccgccacgtt gtgttttgtc tcgataatcc accagttacc cgggaaatcg ctgttcttcg
     1441 ccttcgctgg cagccagcga gcgccgaatt tcgccttgat tgcgtctttc gcacggaaaa
     1501 gaacgccttt catgcctgag gcttcctgaa gcccaaatac ctcgccagcg gcgaattttg
     1561 gtgcgtacat catcttcagg tcggcagtgg atacgcgata attcagacca agagactgag
     1621 ctatgctggt ggtatcaccc tgtattgatg ataactcttc ttgtttctcg tttctggcgg
     1681 caatttcttc ctccgtgatg ttgcctaggg ccaggtttat ccgatcagcg tcttcctgtt
     1741 tctcttcatc ggtgcgcccg gaaagtaccg tgttaattct gtgcaatatc tccacatggt
     1801 tcttgcgcat gctgagcaat tccggcgtaa cctcgttaag gtccaccagc ccaaggatgg
     1861 caaggtcgga aacctgctct ttaaatggat aaggctctgg cagcgtgaag ggcgggtctg
     1921 tcggccccga aaaaactcat cgtcgcttcc tgccctgata cccgtgcagc ttcaggcggg
     1981 ctcttctctc ccaaccttcg aactaccatc ctgctctcct cccgctacct gccacataaa
     2041 atgtcgggga ggagaaataa cactgactca tccctctgct gaactcatga gcactgtcct
     2101 gcgcgaactg atgcgggggc cggtatgata aatcttcccg caggcacaaa aatctggctg
     2161 gttgccggta tcaccgatat gcgcaacggc ttcaatggcc tcgccgcaaa ggtgcagacc
     2221 gcgctgaaag atgacccgat gtccggccac gtcttcatct tccggggacg cagcggcagt
     2281 caggtaaaac tgctctggtc caccggcgat ggtctgtgcc tgctgacaaa gcgactggaa
     2341 cgtggtcgct tcgcctggcc ctcagcccgc gatggcaaag tgttcctgac gccggcgcaa
     2401 ctggcgatgc tgatggaagg tatcgactgg cgacagccaa agcggttact gacatccctg
     2461 accatgttgt agcgctcttt atcctggttg tcgcagaata agcctggtaa aatacaggct
     2521 tatgaacgat acctcttctg acgacatcct tctgctgaaa cagcgcctgg ccgaacagga
     2581 agcgttgatc cacgccctgc aggaaaagct gagcaaccgg gaacgcgaaa tagaccatct
     2641 gcaggcgcaa ctggataagc tgcgccggat gaacttcggc agtcgttccg aaaaggtatc
     2701 ccgccgtatc gcgcaaatgg aagctgacct gaacctgctg cagcaggaaa gcgatacgct
     2761 gaccggccgg gtggatgacc cggcagtgca gcgcccgctg cgtcagaccc gcacccgcaa
     2821 accgttccct gagtcactcc cacgggatga gaagcggctg ctgccgactg agccgtgttg
     2881 cccggagtgc ggtggttcgc tgagttacct gggtgaggat gccgccgaac agctggagct
     2941 gatgcgcagc gccttccggg ttatccggac agtccgtgaa aagcatgcct gccgtcggtg
     3001 cgatcgcatc gttcaggccc cggctccttc gcgccccatc gaacggggga tcgccggacc
     3061 ggggctgctg gcccgagtgc tgacctcaaa gtatgcagag cacacaccgc tgtatcgcca
     3121 gtcggagatc tatgcccgcc agggtgtagt gttgagtcgt tctgtactgt cgggctgggt
     3181 ggatgcgtgt tgtcgtctgc tggcaccgct ggatgaagcc cttcagcact atgtcctgac
     3241 cgacggcaaa ctccatgctg acgatacgcc tgtcccggtg ctgttgccgg gcaataagaa
     3301 gacgaagacc gggcgcttat ggacgtacgt tcgagacgac cgcaacgccg gatcagcgct
     3361 ggcccccgca gtgtggttcg cttacagccc ggacagaaaa ggtatccacc ctcagaccca
     3421 tcttgcaggc ttcagtggcg tactgcaggc tgatgcctac gccgggttca acgagctcta
     3481 ccgcgacggc catataaagg aagccgcgtg ctgggcccat gcccgccgaa aaatccacga
     3541 tgttcacgtc cgtaccccgt cagtcctgac ggaggaggcg ctgaaacgga tcggcgagct
     3601 gtatgccatc gagtcggaga tcagaggtaa aagagcagag gaacggcagg cagtccggca
     3661 ccaaaaagtg ctgccgctgc tggcgtcact ggaggggtgg ctgcgggaga aacagaaaac
     3721 cctctcaagg cactcagaac tggcgaaggc gttcgggtat gcgctgaacc agtggccggc
     3781 gctgatccgc tatgcagaag atggctgggt ggaggtggat aataacattg ccgaaaacgc
     3841 cctacgacgg gtcagtctgg ggagaaaaaa ctggctgttc ttcggctcgg atcatggtgg
     3901 tgagcgcggt gcgtcactgt acagtcttat cgggacgtgc aaattaaacg gcgtggatcc
     3961 ggaacgctat ctgcattatg tacttgatgt catcgccgac tggcctgtga accgggtggg
     4021 tgctctgctc ccgtggcggg ttactctgcc tgcctgacca gcttcacttc gtcaatacgg
     4081 ttctcactgc acgcttacgt tcaaaacacc gaaagagata attgaaaggg gtgttgcatt
     4141 gacagattga atct
//
LOCUS       DAOFQD010000231         4107 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.234, whole genome shotgun sequence.
ACCESSION   DAOFQD010000231 DAOFQD010000000
VERSION     DAOFQD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.234"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..2521
                     /locus_tag="QBF46_004681"
     CDS             <1..2521
                     /locus_tag="QBF46_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDQ6676765.1"
                     /translation="FLFPSGPGEIRERANQRLWKKLAALPDSWQTARVTELLDIPEGQ
                     RTSPLEQLKKGPVTVSGPAFTEALERYIRLRNLEFSRLNFTGLPAIQLRNLARYAGMA
                     SVKYIARMPQQRKLAVLTAFVKAQETAALDEAVDVLDMLILDITRAAKKTGQKKRLRT
                     LKDLDRAALILARACSLLLDEQADDAELRETIFNSIPKSRLAESVCKVNELARPQNNN
                     FHDEMVEQYGRVKRFLPAVLRDLHFQAAPAGEHTLSAIHYLTELNCSKKRILDNAPEH
                     IITGPWKRLVYDAEGRIQRAGYSLCLLERLQDALRRRDIWLENSDRWGNPREKLLQGE
                     EWQAQRVPVCRALGHPTDGHKGVQQLAVQLDETWKTVASRFEGNAEVHICHDGKHPSL
                     TISSLEKLEEPTSLHRLNSRVRLLLPPVDLTELLLEIDARTGFTREFTHVSESGARAQ
                     DLHISLCAVLMAEACNIGLEPLIKHNIPALTRHRLSWVKQNYLRAETLVSANARLVDF
                     QSTLELAGRWGGGEVASADGMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGF
                     HGIVVPGTLRDSIFVLEGLLEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLA
                     DAGEAVFWRVDKSANYGALDELARGCADLSKAEDQWDEMMRTAGSLKLGTIHASELIR
                     SLLKSSRPSGLAQAIMEVGRVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAIC
                     YGQRGEIRKRYREGQEDQLGALGLVTNAVVLWNTLYMQEALSWIRSNGEEIGVEDIAR
                     LSPLMHGHINMLGHYTFTLPEDILKGELRELNFNLNNELTS"
     gene            3126..3455
                     /locus_tag="QBF46_004682"
     CDS             3126..3455
                     /locus_tag="QBF46_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDQ6676766.1"
                     /translation="MFSVVYHPEAREEATALPVKIRVKFDRLIGKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            3436..3717
                     /locus_tag="QBF46_004683"
     CDS             3436..3717
                     /locus_tag="QBF46_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6676767.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVIALY"
BASE COUNT         1041 a         1028 c         1142 g          896 t
ORIGIN      
        1 tttccttttt ccaagcggac ccggtgaaat acgcgaacgg gcaaatcagc ggctgtggaa
       61 gaaactggcc gcgttgccgg acagctggca gaccgcccgc gtgacggagc ttctggacat
      121 tcctgagggg cagcgtactt caccgctgga gcaactgaaa aagggaccgg tcaccgtcag
      181 cggcccggca tttaccgaag cgctggagcg gtatatccgg ctgcgaaacc tggaattttc
      241 ccgactgaat tttaccggtc tgcctgctat acaactgcgt aatttggccc gttacgcagg
      301 catggcgtcg gtaaaatata tcgcgcgaat gccacagcag agaaaactgg ccgtactcac
      361 agcattcgtt aaagcgcagg agactgcggc gctggatgag gccgttgatg tgctcgatat
      421 gctgatactg gatatcactc gtgcggcgaa gaaaacgggg cagaaaaaac ggctcaggac
      481 gcttaaagat cttgatcgtg ctgcgctaat attggcgcgg gcatgttcat tgttgctgga
      541 tgaacaggct gacgatgctg aactgaggga gaccatattc aacagcatac cgaaaagcag
      601 gctggcagaa tccgtctgca aggtaaatga gctggcccgg cctcagaaca acaatttcca
      661 tgacgaaatg gtggagcagt acgggcgggt aaagcgcttt cttccggcgg tattgcgcga
      721 cctgcatttc caggcagcgc cagccggaga acatacgttg tccgccattc attatctgac
      781 cgaactgaac tgctcgaaaa agcgcatcct ggacaatgcg cctgaacata ttattaccgg
      841 cccctggaaa cgcctggtat acgatgcgga gggccggata cagcgtgccg gttactcgct
      901 ttgtctgctg gagcgccttc aggatgcact acgtcgaagg gacatctggc tcgaaaacag
      961 cgatcgctgg ggaaatcccc gcgagaagct gttgcagggg gaagaatggc aggctcagcg
     1021 ggtccccgtt tgccgggcgc tgggacatcc cactgatgga cataaaggcg tgcaacagtt
     1081 ggcggtccag ctagatgaaa cctggaaaac cgtcgcatcc cgctttgaag gaaatgcgga
     1141 ggttcatatt tgccatgacg gtaaacatcc ttccctgact atcagcagcc tggagaaact
     1201 ggaggagcca acatcgttgc atcgtctcaa cagtcgggta aggctgctac tcccgccagt
     1261 agatttgacg gaactgttgc ttgaaataga tgccagaacg ggatttacac gtgagtttac
     1321 gcatgtcagt gaatccgggg cccgggcaca ggatctgcac atcagcctgt gcgcggtcct
     1381 gatggcagag gcctgcaata tcgggctgga accgctgata aagcacaata taccggcgct
     1441 gacgcgccac cggcttagct gggtaaaaca gaattacctc cgggcagaaa cgctggtcag
     1501 cgccaatgcc cgcctggttg attttcagtc cacactggag cttgctggcc gctggggtgg
     1561 tggcgaagtg gcttcagctg atggcatgcg ctttgtcacg ccggtgaaaa ccgtcaattc
     1621 aggacctaac agaaaatatt ttggttccgg acgtggcatc acctggtaca acttcgtctc
     1681 tgatcagtac tctggattcc acggcatcgt tgtccccggc acattacgag attccatttt
     1741 tgtgctggaa ggccttctgg agcagcagac agggctgaat ccggttgaga tcatgacaga
     1801 cacagccggt accagcgaca ttatttttgg cctcttctgg ctgctgggat accagttttc
     1861 cccccggctt gccgatgccg gtgaagcggt attctggcga gtggataaat cggcaaatta
     1921 cggtgcactg gacgaactgg cacgtggttg tgccgatctg tcgaaagccg aggatcagtg
     1981 ggatgagatg atgcgaaccg ccggttcgct gaaactgggc accattcatg cttcagaact
     2041 cattcgctct ttgctgaaaa gctcgcgccc atcagggctg gcacaggcga tcatggaagt
     2101 ggggcgcgtc aacaagacgc tgtaccttct taattatatt gatgatgagg attatcgtcg
     2161 gcggatcctg acgcagctaa accgggggga aggccgccat gctgtggcga gggcgatctg
     2221 ctacgggcag cgcggtgaga tcagaaagcg ctatcgtgaa ggtcaggaag atcagctggg
     2281 ggcactgggc ctggtcacta acgcagtggt actgtggaac acactttata tgcaggaagc
     2341 cctgagctgg atacgcagta atggagaaga aatcggggtt gaagatatcg cccggttgtc
     2401 cccactgatg cacgggcata tcaatatgct ggggcattat acgtttacgc taccggagga
     2461 tattttgaag ggggaactga gagaactaaa tttcaattta aacaatgaat taacttctta
     2521 gcgtacgttt tcgttccatt ggctctcaaa ccccaaaaag tggataaccg aacagcatcg
     2581 gcgcgagcgc ctcaaaagta gctccccacg ctaaagcgcg gggcattcag aacggaatcc
     2641 ctcgatggct aacagctcgc ctgttagcca tcgagggatt ggacaaatcc ggccacgccg
     2701 gatacgtcag caaatatcgc tgaagaatta gcttacacgc tcgtaagcgg ctcttacgac
     2761 ccgctaacgc ggagatacgc cccaactgcg gcttcggcct tgttgtgacc actccgaccg
     2821 cgcacaaaag cctctgagcc gcttttagcg gaatatggct ttgcagggtg aagggatgga
     2881 taggcgatcc ctttcgatcc ctcactggta tcggcggcta accctcggtg ctacctgtca
     2941 tttcatcctg ccgccttaca tgcgctaacg cgcataaatc cccgttcctc agtggaacga
     3001 aaactcacgc taacagggta gtgaccgggt agccacgata actgccaaca aaattagctt
     3061 ataggttaat tctaccttgt ctgaaattag cctataggtt aatattaata gaaatgggag
     3121 gtcacatgtt tagcgttgtt tatcacccgg aagccaggga agaagccaca gcattacccg
     3181 taaaaatcag ggtgaagttt gaccgtctca tcggcaaact ggagtatgac gcccgattac
     3241 tgcgggagcc ggacacgaag cccctgggcg atggactttt tgagatccgc acgatgggga
     3301 cggacatagc caggggaatc tgggtatacc acaaaggcaa taccatcatc atgctccgtg
     3361 ttttcatcaa gaagtcacag aaaacgccag cgaaagaaat cgatctcgcc aaaaagcggt
     3421 tagcggaggt acttaatgaa actggtaagc cataaagaac tgcataacga gtggatgcag
     3481 gacgatgaat atcgcgctgc atggcaggaa gaagaacgta aggaaaagct cagggagctg
     3541 cttgctgaat ggcgcaagca tgacaacctg acaaaagcgc aggttgctga acgcatgggg
     3601 gttacacctc cggttatttc tcgcctggaa aacaacatca ccaaagccag catcgacaca
     3661 ctgacccgct acgctcacgc ctgcggcatc aagaaacctg tcattgccct gtactgaata
     3721 gccagccctc cggggctggc ctttttgttt cggtcagtcc caaaattttg ttaaagcaag
     3781 tttaaaatgt cacctgtttc tccaaagttg aaatgtcact tctaagccaa tctatttata
     3841 cataatcttt acatctttcc ccggtgacaa agtgctaatt ggtctgtaat gttcaatgtg
     3901 tggcaggcaa gccgccggtg acttaggcat aagttgctga tgggtcgaaa agaatctccg
     3961 agactgcctt gtgggcaaac ttgtcattgg cagggccgat ggtgagggtt gaaggtcgtt
     4021 ctggaaagct cgaaacgtgg gaacaatacc ggttacgtaa accacgaact gtccaaggct
     4081 gcggcagtat aaatacgcca gatatta
//
LOCUS       DAOFQD010000232         4103 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.235, whole genome shotgun sequence.
ACCESSION   DAOFQD010000232 DAOFQD010000000
VERSION     DAOFQD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.235"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(327..749)
                     /locus_tag="QBF46_004684"
     CDS             complement(327..749)
                     /locus_tag="QBF46_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6676768.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVI
                     DTSELIRVFGSLDGVIDAVKHDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAV
                     KARDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
     gene            complement(749..940)
                     /locus_tag="QBF46_004685"
     CDS             complement(749..940)
                     /locus_tag="QBF46_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDQ6676769.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDD
                     AEHLFRTLSSRLDALQDGN"
     gene            complement(1420..1569)
                     /locus_tag="QBF46_004686"
     CDS             complement(1420..1569)
                     /locus_tag="QBF46_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676770.1"
                     /translation="MPSEYDEQRDATDAPLPGEYDVFDLMAQVTEENLHAPVDSGPPV
                     GREAL"
     gene            2130..2273
                     /locus_tag="QBF46_004687"
     CDS             2130..2273
                     /locus_tag="QBF46_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDQ6676771.1"
                     /translation="MHEPPVQSDCCACSGNYQLESNPERYDKAPLAAATGKQDVREMY
                     LSS"
     gene            complement(2482..3210)
                     /locus_tag="QBF46_004688"
     CDS             complement(2482..3210)
                     /locus_tag="QBF46_004688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676772.1"
                     /translation="MALMVTRRYLLGGEYPSEEFVQASLEKYFFRQGFDIDTGSYIDL
                     ICRDKESRDVVWHIEVKGKTSQPGLDFRTCLGQLVQRMTKDNINYAIAVPRIKQYERL
                     IEETSILAVSKLNLHWIFVGPDGSVTIKEPDSSTMKNGNGNGNGREVSLVNGGFKRFI
                     TGDFMPTCSYGKAPVVFKSSRREMWDWLDDFVISNGVIPSIADIKEEAESRSGKWTLS
                     NVKIEYQFWKIYHLYKGHFFYQSE"
     gene            complement(3281..3580)
                     /locus_tag="QBF46_004689"
     CDS             complement(3281..3580)
                     /locus_tag="QBF46_004689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676773.1"
                     /translation="MATATNFDAWLDDVDGDYEEVMALYDSVQNVSDMGLYQCVEGGR
                     GDAWVVSSNHHPEALFLASAVARDTFLKLIRERLCGGEDVDSWYGFQRNISNDHS"
BASE COUNT         1166 a         1005 c          859 g         1073 t
ORIGIN      
        1 aaaaaaacgc caaagcgacg attcgactct agagtcaagt cgtcataagt tactgatagt
       61 gtggagtgag tcgtcgatga ctctatgacg agtcgtcggc aagtcgtaca cgaatcaccg
      121 acgacttgga ttgaccttac agaaggtaat actgcgtaat ttaataacaa ccaactgact
      181 gatattaatg agttatctgc aaaaatttaa ttttgcgtgg ggtgtggata tttttagtgt
      241 gaggagcgtt cagagatctg gcgatttttg ggaaggttca cgaacgcccc ggcatggccg
      301 gagaggaaaa ctcaagcatg gcgggatcag gattttttcc agaatttcca ccacggggaa
      361 tggggtggtg aagaaggttc gtgcttgtgc tccagaagct gcatagcctg acgaagggaa
      421 tcaatatgct catctcgagc cttaacagcc cctttaagac catcgttttc agcttcaagt
      481 aaagcgattc tatgttgtaa atcatgtaaa ccaacgctgt taatacctgt agattctgcg
      541 tcacttttta catcatgttt tacagcatcg attacaccat caagagaacc aaacactcta
      601 ataagctctg atgtatcaat aactttaata cccttcccgt gctttacaac agaggtgtaa
      661 cttaacttac cactgttaat catcttgtaa atagtagggc gactaacctt aacaagccgt
      721 gctgcttcac taatagacac tctggccatc agttaccatc ctgtaatgca tccagtctcg
      781 aggacagtgt acggaaaaga tgctccgcat catcgtggag tttctcacaa aggtcggcct
      841 cagcatcgag atcaagctcg ttaagcttct ccagcaggag cagagactga ttctggatga
      901 atttagccat gttcagcgct gttttctgtt gtttcgtcat aagggccctc agagcatctg
      961 tgctcagtgt aaaccggtaa gggggtcgga tgtacaaatg gttcagagaa cggatctgag
     1021 gtaatcctgt agttattttg tagtttatct gtagtcatcg cactaaaaat caggattaaa
     1081 tcatcaactt ttgtagtctt cctgtagttt gcctgtagtc cggaaaaata atccgttcat
     1141 gcaagcatga acgaagcagt taaagacgct ttttcgtcac caatgctcct taacctgcgc
     1201 agtacacccg gccagaatga taattttttg ccccttaaat ggtcatacac ctaacgcagc
     1261 cgcgattttc atcgcaactg catgaattaa aaggtaaaaa ctgaaatctc cgtttattca
     1321 tgtaatacgc aacgtattac aatcacctgt aattacatga aaataaagaa ataaaatgta
     1381 ataccggcgt attaccgcgt acgcgggcaa gcgaaaaagc tataacgctt cccggccgac
     1441 aggcggcccg gaatccacag gggcgtgcag gttctcttca gtaacctgcg ccattaaatc
     1501 aaaaacgtca tattcgccgg gaagcggagc atcagtcgca tcccgttgtt catcatactc
     1561 agatggcatg gggtgatatc cagtagctga gagattgtta gcgggtttcg gggcgcagcc
     1621 ctgaaccagt cacgtagcgc tagcggagtg tatactggct taatatgtta cggatgagta
     1681 acacgtaatg aaagtgcttc atgtggcagg ggaaaaatgc agcggcaggg cgtacaggag
     1741 aaaatggagg ttacggtgat atgacgcctc ctcgctcact cggtcgctga cgctcctgcc
     1801 gtgagtctgc ggcgggcgtt acctgcacac ggataagagc aaagacatac ggcatgacaa
     1861 gcggggcagc aaaaggctgc ggagctgccg tttttccata ggctccgccc ccctgacaag
     1921 catcacgaaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagatc
     1981 ccaggcgttt ccccctggta gctccctcgt gcgctctcct gttcctgcca ttcggtttac
     2041 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     2101 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct
     2161 gcgcctgttc cggtaactat caacttgagt ccaacccgga aaggtacgac aaagcgccac
     2221 tggcagcagc cactggtaaa caggatgtgc gagagatgta tttgagttct tgaagtgaag
     2281 acctaactga ggctacactg gaaggacgga tttggtgaca tgtctcgcac aagactgtta
     2341 ccacggttca taaacttgcc ggttaatgaa ccttcgaaaa accaccttgc cgggtggttt
     2401 tttcgttttc cgagaaagag attacgcttt gagcgaatga atctcaagaa gatcatggca
     2461 tttgatgaac aaatgagtca gctactcaga ttgatagaaa aaatgcccct tatacaagtg
     2521 atatattttc caaaactgat actctatttt aacattagaa agcgtccatt tcccactccg
     2581 cgattcagcc tcttctttaa tatcagcaat acttgggatc accccatttg aaataacaaa
     2641 atcgtccaac caatcccaca tttctcgccg agatgattta aatacaacag gagctttccc
     2701 atatgagcaa gttggcataa aatcccccgt aataaatctt ttaaaccccc cattcacaag
     2761 agaaacttct cttccattac cattaccatt accatttttc atcgtagagc tatcaggttc
     2821 cttaattgtc acactgccat caggcccaac aaatatccag tgtaaattca atttactaac
     2881 agcaagaatg ctcgtttctt ctattaatct ttcatactgt tttattcttg gaacagcaat
     2941 agcataatta atattatctt ttgtcattct ctgcactaat tgtccaagac aagttcgaaa
     3001 atccaaccct ggctgtgatg tttttccttt tacttcaata tgccacacaa catccctaga
     3061 ttctttatca cgacaaataa ggtcaatata agaacccgta tctatatcga acccttgtcg
     3121 aaaaaaatat ttttctaaag aagcttgaac aaattcctcg gagggatatt caccacctag
     3181 taagtacctt ctcgtcacca taagagccac aacccctacg catcctattt actataacgt
     3241 catattttaa gagttcccac aaggggaact ctctgtacga ttacgagtga tcattcgata
     3301 tatttcgttg aaatccatac catgaatcaa catcttcgcc gccacatagc ctttctctaa
     3361 tcaatttgag gaaagtatcc ctagccacag ctgatgctag aaaaagagct tctggatgat
     3421 gattagagct cacgacccaa gcatcacccc gaccaccctc aacacactga tacaacccca
     3481 tatcacttac attctgaacg gaatcataaa gagccataac ctcctcataa tcgccatcaa
     3541 catcatctaa ccacgcatca aaattagtag cagtcgccat gccaatcctc cattttttag
     3601 ttttcaggag ttaatttaca tagtgatagg ggctgttaag caaataagtt tttacccttt
     3661 ttgcctcatg ctctaaaagt gagcaagaca ggcatttacc ataacgtcgc attacacgca
     3721 ccgctcaaac gctcgagaaa cgattctggg gcgtaaccca ttaaatccta cgtcttaccc
     3781 cctgaaaacg cctcaaaatc gcgctgatgg gttttacggg catgaattgt tatgcaccag
     3841 actggctgga ggtagatttc atcctgaatt cacgccagac aactcaaaac ccgcaccaaa
     3901 cccgactgaa aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc
     3961 gcagccggaa aaccggtttt cagcacgttc aggaatcggg ttcgtcgacg gccggcaatc
     4021 acgttatggc aagtcgccgg acgactcacc acacgattca ccggcgattc gactcgtgac
     4081 ttgactctag agtcaccacg gtc
//
LOCUS       DAOFQD010000233         4010 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.236, whole genome shotgun sequence.
ACCESSION   DAOFQD010000233 DAOFQD010000000
VERSION     DAOFQD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.236"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            220..1170
                     /locus_tag="QBF46_004690"
     CDS             220..1170
                     /locus_tag="QBF46_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDQ6676774.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            1180..2562
                     /locus_tag="QBF46_004691"
     CDS             1180..2562
                     /locus_tag="QBF46_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDQ6676775.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKLHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMVVQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            2939..3988
                     /gene="yahK"
                     /locus_tag="QBF46_004692"
     CDS             2939..3988
                     /gene="yahK"
                     /locus_tag="QBF46_004692"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDQ6676776.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDNFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT          972 a         1020 c         1152 g          866 t
ORIGIN      
        1 ggatgcggcg tggacgcctt atccggccta cgagtggcgc gagaatctgt aggcctgata
       61 agcgtagcgc atcaggcatt tgtcaccatt gccggatgcg gcgtgaacgc cttatccggc
      121 ctacgagtgg cgcgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
      181 ggtcataacc ccatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
      241 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
      301 gaggcggtga aagccgtcgc cgatacggtg ctggaaatgc tggcttccga ttacgacatt
      361 gtgctgaccc acggcaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
      421 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
      481 ggcatcggct atctgatcca acaggcgctg aataaccggc tggcgcgtca cggcgagaag
      541 aaagccgtca ccgtggtgac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
      601 cccaccaagc ccatcggcgc attctttagt gacagccagc gtgacgaatt acaaaaggca
      661 aaccctgact ggtgttttgt tgaagatgcc gggcggggct atcgccgcgt ggtcgcctcg
      721 ccggaaccga aacgtattgt cgaagcccct gccattaaag cgctgatcca acaaggtttt
      781 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgacgc gggagattac
      841 caaagcgtgg acgcggttat cgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
      901 cacgccgaca ttcttgtgat caccactggc gtcgaaaaag tgtgtattca ctttggcaaa
      961 ccgcagcagc aggcgctcga tcgggtggat attgccacca tgacccgcta tatgcaggaa
     1021 gggcatttcc cgcccggcag catgttgcca aaaatcatcg ccagcctgac atttttagaa
     1081 caaggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggc
     1141 gaaacgggca ctcatattat taaaacgtaa ggacgtaaga tgaaagaaag caatagccgc
     1201 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctct
     1261 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     1321 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     1381 gaaaatggcg tggcggtgca gacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     1441 aaaattgtcg ccctgcgcga gaacaagctg catccggacg ccacgctgcc gcactatgac
     1501 gctggcggta agctgatgct gcccaccacc cgcgacatgc atattcatct cgacaaaacc
     1561 ttttacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccaccat ccaggacatg
     1621 atcaaactcg agcagaaaat gctgccggaa ctgcaaccgt acactcagga gcgggcagaa
     1681 aaactgattg atttattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     1741 gaaccggttt ccggcctgaa aaatctgcaa aatttgcagg cggtgctggc gcgacgtcag
     1801 gcgggctttg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatct
     1861 gaacctttaa tgcgtgaagc gatgcaggcg ggggcgcatt acgtcggcgg cctggacccg
     1921 accagtgttg atggcgcgat ggaaaaatcc ctcgacacca tgttccagat tgcgctggac
     1981 tacgacaaag gcgtcgatat tcacctgcac gaaaccactc cggcaggcgt ggcagccatc
     2041 aattatatgg ttgaaacggt agagaaaacg ccacagctga agggcaagct gaccatcagt
     2101 cacgcctttg cgctggcaac gctcaacgag caacaggtag atgaactggc gaaccggatg
     2161 gtggtgcaac aaatttctat tgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     2221 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     2281 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctgtacgc gcagctctat
     2341 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     2401 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgacgc cagctttgtg
     2461 ctggtggacg cctcctgttc tgccgaggcg gtggcgcgta tctcgccgag aacagcaacg
     2521 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     2581 gcaacggtga atagtaagag atttaagccc cagggagtgg ggcaatctga atatgagtgt
     2641 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     2701 tatttgtcta cgtgaaaaga tcatcagttt taccgatttt agtcttttac agatagcaaa
     2761 tatcacactt acaggcgctc gccttatggg gaacgttgct gctctgtttc attcaggctc
     2821 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     2881 tgccgcgatg acatatcagg cgttgccaaa tacacatagc taatcaggag taaacacaat
     2941 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     3001 ccggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtttgcca
     3061 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtgccggg
     3121 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaat atgcgccggg
     3181 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgaaga
     3241 cgggttggaa aactactgtg atcacatgac cggcacctat aactcgccga cgccggacga
     3301 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     3361 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     3421 caccacgtat tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     3481 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     3541 ggtggcattt accacttctg aggcaaaacg cgaagcggca aaagccctgg gggccgatga
     3601 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     3661 tttgaataca gtagctgcgc cacataatct cgacaatttt accaccttgc tgaagcgtga
     3721 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     3781 cctgatcatg aaacgccgtg cgatagccgg ttctatgatt ggcggcattc cagaaactca
     3841 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     3901 cgatcaaatt aatgaagcct atgagcgaat gctgcgcggt gatgtgaaat atcgttttgt
     3961 tatcgataat cgcacactaa cagactgaaa aaattaataa ataccctgtg
//
LOCUS       DAOFQD010000234         3923 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.237, whole genome shotgun sequence.
ACCESSION   DAOFQD010000234 DAOFQD010000000
VERSION     DAOFQD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.237"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(769..1281)
                     /locus_tag="QBF46_004693"
     CDS             complement(769..1281)
                     /locus_tag="QBF46_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676777.1"
                     /translation="MAKVDQTKANMLLSATKTMSLGANKNSIRILYGSHVAENIDFRS
                     EPQQNVKKGIRGWSRRSDNRRAFSAVIDVAVNYFGFDPIHPESSFRNIDFNRIMSVLH
                     NLGYFVCAPEVNKIIEILTSMKEIEELETCAKCKRPYIVPYTNDTHCPRCRLSSTVSA
                     IARYSQEELD"
     gene            complement(1287..2084)
                     /locus_tag="QBF46_004694"
     CDS             complement(1287..2084)
                     /locus_tag="QBF46_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trhR"
                     /protein_id="HDQ6676778.1"
                     /translation="MSESDNITRNLLGKLLRTEISIIRSYRAFLMLLPIHGSSMYQTG
                     SPLLQRRLFGTGFGAMADNAFEVETRPGSFLVPRSLGKEISWDKFFMAVVDGDTNVIR
                     EYDSDDTDFGIYNAGEKVTLLNGQEDCYNPSKIQQSRSKCVDIQNDYFMQIFFMSMLA
                     PEFVSIFFGLKSATVDAIKDVGVSSLKAINDVVLFPRIIAFTPGLGDDSATLKSKVFA
                     LAYELSADIRLGKVSDDLMELLRYDTMFTCHRQDVFNSLANKVLLKD"
     gene            2839..3324
                     /locus_tag="QBF46_004695"
     CDS             2839..3324
                     /locus_tag="QBF46_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000045622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDQ6676779.1"
                     /translation="MSNSKLIKSLELSRSLMRSQIEVLREAAEDLCTNYWIEWKERNH
                     LEINLPRPNNTLKREYLGSYAPKIELIGNAKKVTITWHKFSPFKTRSPSHMSKRISAM
                     KSGKYSKSCFVNHAFWEYEMIEKTEGVLEPYRELLEFYHTAYIELGRKIRKYSKSEVA
                     Q"
     gene            3321..>3923
                     /locus_tag="QBF46_004696"
     CDS             3321..>3923
                     /locus_tag="QBF46_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676780.1"
                     /translation="MTDVTINESKQKTPDVNARSSQTPEDSRRATQSKNEHYGMATVT
                     ITPGTPDFNRFLTARNRSVIRGFNDVSIAISSLFRTVNAVKHPELVQAIQDWFNELHD
                     ENNLMKQNLEAHIATIKVDESDPFFSSTEFSPFRFESVQLNFNNQNTMRFYKHIFEMN
                     NLLTQMYKFNSLGQLPVSDYNVMAHNIIRSLNMYIERVKKT"
BASE COUNT         1279 a          815 c          767 g         1062 t
ORIGIN      
        1 gtcgtcatac cagaaaaaac ctttcttaaa ggcttgtcct gacacaaatg gggtgtcttt
       61 gataagggtc tggtcttcat ttgcatggac gccagttata aacaaagaca tcagcactaa
      121 catctttgaa ggcagtaacg ttaatcgagt cattcaccga tctcctcatg gaatagaggt
      181 atgatggcat aaaaaaaatc accgggagtt cgataacgga gtagatttca tgcgttaaaa
      241 aaccagaaat gtgacgccga caaagcccca aaaacgacag ttcaacacag tgtcccggta
      301 aagacatggt ctgtaagaga gaaatggatt aattatatga cggtacagta atgcaaattc
      361 gcgttgtcat tcagacaaca ctatccagat tatgaacgat ccccgtactt tgctggttaa
      421 cgcataaggg aatttatact taattgaata cctataagaa acgaaattgg caggcgcatc
      481 ataaaatctg gacttttcaa agaccgtaat gaatcaaaga atggcaattc atgttagtta
      541 tacttacaga aaatgaacgt tggaaggata gagcagacaa aaaaatacgg atggtagtaa
      601 tatttaacac agtatgtttt agatacgtca gaacggaagc cgtctgaaaa taaacagcaa
      661 catgaggaac catgtgtaac ggtatgcaag ccaaagattt aatagataaa gacgacgcag
      721 tctttagatt tttggtctca gcttaaaacc actcacataa agtaatagct aatctaactc
      781 ctcctgagaa tatctcgcta ttgcagagac cgttgacgac aatctgcacc gagggcaatg
      841 agtatcattg gtatacggga caatgtaagg ccgcttgcat ttcgcacaag tttcgagttc
      901 ctctatttct ttcattgacg tgaggatctc aatgatttta ttgacctcag gggcgcagac
      961 gaaataccca aggttatgca gcacgctcat gattcggttg aagtcaatat tgcggaagct
     1021 ggattccgga tgaataggat caaaaccgaa gtaatttacc gcgacatcaa taaccgcaga
     1081 aaatgctctg cggttatccg aacgacgact ccaccctcga ataccttttt taacgttttg
     1141 ttgaggttcc gatctgaaat caatattttc tgcaacatgg cttccataaa gaattcttat
     1201 tgagttttta ttcgcaccaa gtgacatcgt ttttgtagcg ctcaacaaca tgttagcttt
     1261 agtttgatcg actttagcca tttttattaa tccttcaaga gcactttatt tgccaaagag
     1321 ttgaacacat cctgacggtg gcatgtaaac atagtgtcgt aacgcaatag ttccattaag
     1381 tcgtcactaa ctttacccaa acgaatgtcg gcagataatt cgtatgccaa ggcaaacacc
     1441 ttagatttta atgttgcact atcatccccc aaccccggtg taaaggcaat tattcgtggg
     1501 aatagaacga catcgttaat tgctttgagt gatgacacac caacatcttt tattgcatct
     1561 acggtagcag actttaatcc aaaaaatata ctgacaaact caggtgcaag cattgacata
     1621 aagaagatct gcataaaata atcattttgt atgtctacac acttggagcg tgactgctga
     1681 attttgctcg gattataaca atcttcctgc ccattcaaca aagtaacttt ttcccctgca
     1741 ttgtaaatgc cgaagtcagt atcgtcactg tcatattcac ggataacgtt agtgtcacca
     1801 tcaacaacgg ccataaaaaa tttatcccac gagatctctt tacctaaaga acgtggaacg
     1861 aggaaacttc cgggacgtgt ttcaacttcg aatgcattat cagccattgc cccaaacccg
     1921 gttccaaata gacggcgttg caggagtgga gaccctgttt gatacataga agatccgtga
     1981 ataggcaata acattaggaa tgcacggtaa ctacgaataa tcgaaatctc ggtacgtaat
     2041 agtttaccga gtaggttacg cgttatgtta tctgattctg acatgtgcgg attgtccttg
     2101 cgtcaataca attgcactaa tcttctacat gaaattaaag tttggttttt gaatgatgaa
     2161 aagtaacttt tggaatttta atactgccta tttgagtctt aaaactaacc attcatccat
     2221 tggccttact tgtaacactt tttaaccaaa ctaaccagaa ttttatcaaa aaaccctgcg
     2281 cttgctaacg aaattttaat cgcttaaaaa aatagcaaag cccgaatcgt tatcggcatt
     2341 aagcggcaat aacattagcg aaaaaagcga ttcaggatga atatttttcc gattgattaa
     2401 catgactact acatagggtc acttcttgcc gaaaagtggt cctatgaagc ctcgacatag
     2461 ggtcacttct ttgtgaaaag tgaccctatg tagccaagac atagggtcac tttttttaaa
     2521 agtggcacta tgcgcaaaaa acatagggtc acttttgttt aaaaagtggc actagatata
     2581 tgtataactt agtaagtaaa acagcacttt tcagatcacg gtctcaaaat acccatggca
     2641 gaaaaagtta cttttctgcc gcgcttaaat gcgcccatct cagccgttcc cgtgttcatc
     2701 tggagctgcg cactatctgt attcctcacg cctccgaagc cccacctcaa aattctcgat
     2761 aaaaatcacg gaaaaaagtc ctgtttgtga aaaaaataaa aaccacttcg gtatagtgat
     2821 ttcacatcag gaggtcacat gtctaattcg aaactaataa agtcactaga attatcacgc
     2881 agtttgatgc gttcacaaat cgaggttctg cgggaggcag ctgaagacct ttgcacaaat
     2941 tactggatcg agtggaaaga acggaatcat ctggagatta atttaccccg gcctaacaat
     3001 acgctaaagc gtgagtatct gggttcgtat gcgccgaaaa ttgaattaat cggtaatgcc
     3061 aaaaaagtta caatcacctg gcataagttc agcccgttta aaaccaggtc tcccagtcat
     3121 atgtcgaaac ggatttctgc gatgaaaagc ggaaaatatt cgaaaagctg ctttgttaat
     3181 catgccttct gggaatatga aatgatcgaa aaaacggagg gagtactaga accctacaga
     3241 gaattacttg agttctatca tactgcgtat atcgaactgg gacgcaaaat ccgtaaatac
     3301 tcaaaatcag aggtggcaca atgaccgacg taacaattaa tgaaagcaag cagaagacac
     3361 ctgatgtaaa tgcccgctcc tctcagactc ctgaagatag ccgaagagcc acgcaaagta
     3421 aaaatgaaca ttacggcatg gcaacagtaa ctattactcc aggcacgcca gactttaacc
     3481 gatttcttac ggcacggaat cgctccgtaa taagaggctt taatgatgtt agcattgcta
     3541 tttcatcatt attcaggacg gttaatgcgg taaaacaccc tgaattggtg caggcaattc
     3601 aggactggtt taatgaattg cacgacgaaa acaatttgat gaagcagaat ctggaggccc
     3661 acatcgccac tattaaggtt gatgaaagtg atccattctt ctcatcaact gagttttctc
     3721 cgttcagatt tgaatctgtc cagttaaact tcaacaacca gaacacgatg cgattttata
     3781 agcacatctt tgaaatgaac aaccttctga cccagatgta taaattcaac tctctgggtc
     3841 agctgccggt tagcgattac aacgtaatgg cgcacaatat tatccgaagt ctcaacatgt
     3901 atatcgaacg ggtcaaaaaa aca
//
LOCUS       DAOFQD010000235         3913 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.238, whole genome shotgun sequence.
ACCESSION   DAOFQD010000235 DAOFQD010000000
VERSION     DAOFQD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.238"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1033)
                     /gene="ahpF"
                     /locus_tag="QBF46_004697"
     CDS             complement(<1..1033)
                     /gene="ahpF"
                     /locus_tag="QBF46_004697"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDQ6676781.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTY"
     gene            complement(1278..1841)
                     /gene="ahpC"
                     /locus_tag="QBF46_004698"
     CDS             complement(1278..1841)
                     /gene="ahpC"
                     /locus_tag="QBF46_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDQ6676782.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            2213..2959
                     /gene="dsbG"
                     /locus_tag="QBF46_004699"
     CDS             2213..2959
                     /gene="dsbG"
                     /locus_tag="QBF46_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDQ6676783.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            3168..3913
                     /locus_tag="QBF46_004700"
                     /pseudo
     CDS             3168..3913
                     /locus_tag="QBF46_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1057 a          934 c          905 g         1017 t
ORIGIN      
        1 agtaggtcac gcctttggtg cgatactgat cttcgcccgg aacgttcatg ttgcgccatt
       61 ttgcaccggt cgccacgata atgctgcgtg ctttcagtac cgcgccagaa gctgtttcaa
      121 tctggtgtag accaccttca accgctgccg ggatcagttt gctggcgctc tggctgtcga
      181 tcacatcaac gtcgtattca tcaacgtgaa ctttcagtgc gcctgccagt ttctgccctt
      241 cagtcttcgg tacagaaatg tagttttcga tatcaacggt atcgaggatc tgaccaccaa
      301 aacgttcgcc catcagaccg gtacggatgc ctttacgtgc ggagtaaatt gctgccgctg
      361 cacccgccgg gccggaaccg acgattaata cgtcataagc atcacgcttg ttcagctctt
      421 ctgccgcacg tttttccgcg ccagtatcaa ttttggcaac gatttcagtc aacgtcatgc
      481 ggccctgacc aaactctttc ccgtttacga acactgccgg aacgcccatc acgttgcgat
      541 cggtgatttc gttctggaag gtgccgccgt caattgcagt gtgcttgatg cgcgggttca
      601 atacgctcat caggttcagc gcctgcacca cgtccgggca gttatggcaa gagagcgagt
      661 aataggtttc gaattcaaaa tcaccgtcaa tatggcgaat ctgctccagc agagactgcg
      721 cttctttcga cggatgacca ccggtccaca gtaacgccag taccagcgag gtgaactcgt
      781 ggcccagcgg ggagcctgca aaacgtggcc cctggttgga acctgggttg gtgatcagga
      841 aagacggctt acgcaccggc aagctgttat cttctttaaa ggtgactttg tctgacagtt
      901 ctgcgatttc agccaacagt tccttgattt ctgccgattt agcgctgtca tccagcgtgg
      961 caattaactc aacaggcttg gtcaatttct caaggtaagc cttgagttga gttttcatat
     1021 ttgtgtcgag catgtttatc tcctgggctt taaacatcat catgcaagcg gcctctgcgg
     1081 ctacctggat gcagcttgca tcatggtgcg gggaagaaaa tcgggtgccg cagcacccga
     1141 agaattaggt gaatttccga agtatttcgc gtcagagcaa ggcggctagc ctgtgaatcc
     1201 ccgggagctt acacaagtaa gtgaccgggg tgagcgggcg acgccaacgc cgctatggcg
     1261 tgaaagacga aggaaattta gattttacca accaggtcca gagacggagc cagagttgct
     1321 tcaccttctt tccatttagc cgggcaaact tcacctgggt gagaagctac gtactgtgct
     1381 gctttgattt tacgcagcag gtcagacgcg tcacggccaa tgccttcagc ggtaacttcg
     1441 attgcctgga tgataccctg cgggtcaaca acgaaggtcg cacggtcagc cagaccttca
     1501 tcttcacgca tgttgtcgaa gttacgggtc agggcgccag tcgggtcgcc gatcatcgca
     1561 tatttgattt tagcgatggt ttcagagctg ctgtgccatg ctttgtgggt gaagtgagta
     1621 tcggtagata ctgcgtatac gtctacgccc agtttctgca gttcttcgta gtggtcagca
     1681 acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag ccgggtagaa gaagaagacg
     1741 ctccagcggc cttcggtatc tttttcggtg atttcgatga attcgccgtt tttgaatgcc
     1801 tggtttttaa aaggtttaat tttggtgtta atcaaggaca tctatacttc ctccgtgttt
     1861 tcgatgagat ctaaggtaac caatttttgc cgattcggct aatgcgtttc cattatcaaa
     1921 tcgataagtt ttaccttaca accttcgtaa gacaactttg tgaactttgc accgccagcg
     1981 gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg taatctgaac taccctttgg
     2041 tatcttcagt gcctgcctgc aggctgaggt gttgccctta caaatgcaac aacgacatgg
     2101 attacaacac cctcataaac aaagggcaat cacctgatct aagctcttac ctatgacagt
     2161 gataggttat gccttttact cgacttttgc actgactgaa aaggacaaat taatgttaaa
     2221 aaagatactt ttactggctc tgcttcctgc aatcgccttc gcagaggaac ttcctgctcc
     2281 agtaaaagcg attgaaaaac agggcattac aatcatcaaa acattcgatg cccccggagg
     2341 aatgaaaggt tatctcggaa agtatcagga tatgggcgtc accatctacc tgactccaga
     2401 tggtaagcac gctatctctg gttacatgta caacgagaaa ggtgaaaacc tgagtaacac
     2461 acttatcgaa aaagaaattt acgcaccagc cggacgcgaa atgtggcaac ggatggaaca
     2521 atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc attgtctacg tcttcgccga
     2581 tccgttctgc ccatattgta aacagttctg gcagcaggcg cgcccgtggg tagattctgg
     2641 caaagtgcaa ttaagaacat tgttggttgg ggttatcaag ccagaaagcc cggcgacagc
     2701 agcggcaatt cttgcctcca aagatcccgc aaaaacctgg caacaatatg aagcctctgg
     2761 tggcaagctt aagctaaacg tgcctgcaaa cgtaagtaca gagcaaatga aagtgttaag
     2821 cgataacgag aaactcatgg acgatctggg ggcaaatgtc acaccggcta tctattacat
     2881 gagtaaggaa aatacgctac aacaggccgt ggggttgccc gatcagaaaa cgcttaatat
     2941 cattatgggg aataaataaa aggtaattca tggatggttg aactatatcc ctgcttttca
     3001 accatcctaa aaaagcatga attgctcgct ctgccctaag tggctgtgct attttagaaa
     3061 aacttaatcc tgagttaagc aaaaaaacat aatcaataaa aatatatacg caaaaaacat
     3121 tgattaagtg aatatatcat ggaagaaaaa tataaccgga gtagtgtatg gccaatctct
     3181 acgacttgaa aaagttcgac ttaaatcttc ttgtcatatt tgagtgtatt taccagcatt
     3241 taagcattag caaagctgct gaatcactat atatcacccc atcagccgtg agccagtcgt
     3301 tacaacgctt acgtgcgcaa tttaatgacc ctttattcat tcgctcagga aaaggtatcg
     3361 cgccgacgac aacagggtta aatctgcatc accatcttga gaaaaatctc agaggccttg
     3421 aacaaacaat taatatagtc aataagtccg aactaaagaa aaactttatt atctacgggc
     3481 cctcagctca tttcttgctc taataatagt atgctgatcc gctgcctacg gcaagattcc
     3541 tctgtcgaga ttgagtgcca tgatatcctc atgtcagcag agaatgctga agaactgttg
     3601 gttcaccgca aagccgatct ggtgatcacc caaatgccag tgatcagccg ttccgtcatt
     3661 tgcatgccat tgcatacgat tcgcaatact ctaatttgta gtaataggca tccacgaatt
     3721 actgacaata gcacttatga acaaattatg gcagaagagt tcacccaatt aatatctaaa
     3781 tcagcagggg ttgatgatat tcaaatggaa atagacgaga gatttatgaa tcgcaaaatc
     3841 tcatttcgtg gttcctcttt gctgacaatc attaatagca ttgcagttac agatttactg
     3901 ggaatagtcc cct
//
LOCUS       DAOFQD010000236         3890 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.239, whole genome shotgun sequence.
ACCESSION   DAOFQD010000236 DAOFQD010000000
VERSION     DAOFQD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.239"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            11..493
                     /locus_tag="QBF46_004701"
     CDS             11..493
                     /locus_tag="QBF46_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rhsA"
                     /protein_id="HDQ6676784.1"
                     /translation="MTQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWP
                     KSPAQQQADDNAAKALTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGIT
                     DRVNDIINDRFWSDGKKPDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR
                     "
     gene            514..1356
                     /locus_tag="QBF46_004702"
     CDS             514..1356
                     /locus_tag="QBF46_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDQ6676785.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1398..2342
                     /locus_tag="QBF46_004703"
                     /pseudo
     CDS             1398..2342
                     /locus_tag="QBF46_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            2354..2815
                     /locus_tag="QBF46_004704"
     CDS             2354..2815
                     /locus_tag="QBF46_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDQ6676786.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            3522..>3890
                     /locus_tag="QBF46_004705"
     CDS             3522..>3890
                     /locus_tag="QBF46_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676787.1"
                     /translation="MADKEILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDK"
BASE COUNT         1283 a          675 c          862 g         1070 t
ORIGIN      
        1 ggggaggtat atcactcagg atccgattgg gctgaagggg ggatggaatt tttatcagta
       61 tccgttgaat ccagttacga atacagatcc tctggggtta gaagtttttc ctagaccatt
      121 ccccttgcca attccatggc ccaaaagccc tgcacagcag caagcagatg ataatgctgc
      181 aaaagcattg acaaaatggt ggaacgatac agcatcacaa agaatatttg actctctaat
      241 attgaataat ccgggactag cattagatat aacaatgata gcttctcgtg gaaatgttgc
      301 agacacaggg ataactgatc gtgtcaatga cataataaat gacagattct ggagtgatgg
      361 gaaaaaaccc gacagatgtg acgtacttca ggaactaatt gattgtggtg atattagtgc
      421 taaagatgca aaaagcacac agaaagcctg gaattgtcgt cactccagac agtcaaacga
      481 taaaaaaaga tagcccttgt ggaggttcct gcaatgtcaa atacatacca gaaaagaaag
      541 gcaagtaaag agtatggttt atataatcaa tgtaagaaac taaatgatga tgaattattt
      601 cgcttacttg atgatcacaa ttccttgaaa aggatttcat ctgccagagt attacagtta
      661 agaggtgggc aagacgctgt tagattggca attgagttct gctctgataa aaattatatc
      721 cgtagagata tcggagcatt tatactcggg caaataaaaa tttgcaaaaa atgcgaagat
      781 aatgttttta atattttgaa caatatggca ttgaatgata agagcgcttg cgttcgagct
      841 acggcaatcg agtcaacggc tcagcgatgc aagaaaaacc caatttattc acctaaaata
      901 gtagaacaat ctcaaattac tgcttttgat aaatcgacta atgtcagacg tgctacagca
      961 tttgctattt ctgttatcaa tgataaagca acaattccac tattgattaa tctgttaaaa
     1021 gatccaaatg gagatgtcag aaactgggcc gcatttgcaa taaatatcaa taaatatgat
     1081 aatagtgata ttagggattg ttttgtggag atgcttcagg ataaaaatga ggaagtccgt
     1141 attgaagcaa taatcggact ttcctacaga aaagataaaa gggttttatc tgttttatgc
     1201 gatgagttaa aaaaaaatac tgtttatgat gatatcattg aagctgcggg tgaattaggt
     1261 gataaaacgc tacttcctgt tttagatact atgttgtaca agtttgatga caatgaaatt
     1321 ataacttccg ctattgataa gctgaagcgt tcatagcgtg agttgcctat gcacagtggg
     1381 ggattcccgc cggcacggtg cagatgctcg accggctgga aagtgaaatc ctggctgacc
     1441 gggtgagtga ggaaagccgc cgctggctgg catcgtgcgg cctgacggtg gcgcagatgc
     1501 aaagccagat ggacccggtg tacacgccgg cgcgaaaaat ccacctgtac cactgcgacc
     1561 atcgcggcct gccgctggcg ctcatcagca cggaaggggc aacagcgtgg tgcgcagaat
     1621 atgatgaatg gggcaacctg ctgagtgatg agaacccgca tcatctgcag cagctcattc
     1681 gcctgccggg tcagcagtat gatgaggagt ccggcctgta ttacaaccgc caccgctatt
     1741 atgacccgct gcaggggcgg tatatcactc aggatccgat tgggctgaag gggggatgga
     1801 atttttatca gtatccgttg aatccggtca taaatgtaga tccgcaaggt ttggttgata
     1861 taaatttata ccccgaaagt gatcttatcc attctgtagc tgatgagatt aatatcccag
     1921 gcgttttcac aatcgggggg catggtaccc ccacatctat tgaatccgca acgcgcagta
     1981 tcatgacagc taaagatcta gcatatctaa ttaaatttga tgggaattat aaagatggga
     2041 tgacagtttg gttattttct tgtaatacag gtaaaggaca aaattcattt gctagccaat
     2101 tagctaaaga gttacataca aatgtaatag gacctgacac gctatggacg tggtgggggc
     2161 gaggaactaa tggtaagtta aaaatggata cagtgctaac agcaccaacg aaccttaatt
     2221 caaataagga tctaatggct ataacaacaa aagaccttgg taattggata acatatgggc
     2281 catctgggca ccccatttct aatatgcaag gtacgccaga aaaacccagt gatataagat
     2341 aggttgtaga tgtatgaaag catgcttgtt actatttttt tatttttctc ttattggtca
     2401 attgcatggt gctgatgtga aaataaaaga aaacgaaagt gtgatgggat ctacagcaat
     2461 gacctatgat ttaagtgaag aaaagctgat gaaactgaag tataaatcac aacatggtga
     2521 ttcagaggca tcatttcgac tatatcaata ttactgcttt actaaaaata atattgataa
     2581 acaattgcga ttcttggaaa gatcagcatc tcaggggaat gttacagcgc agtttaatta
     2641 tggggtcttt ttatcagata caaatccaac attatcagaa tattataatt tgaatagagc
     2701 tatttattgg atggaattcg ctgtaaataa cggtaatatt gatgcaaaga gcaaacttca
     2761 agagctcaaa aagctaaagc gaatggatag aaggaagaat aaggagaatc cgtaaaagca
     2821 atagttcatc agactgccgc agcagtagta tgatgatgaa tcagggctgt actataacta
     2881 ttatcggtaa cattaaccgc agcaggggcg gtataacact caggctccga ttgggctgga
     2941 aggtaggtgg aatttttatc agtatccgtt gaacccgatt tctggaatcg atccccttgg
     3001 gattagccac ctgtttatac agcataactt taggtatgct ttcgtgtgtt tcagatatac
     3061 catgtgatga taatttttat gatgtgctta gtattccagt cgcttctggt aataatatgc
     3121 aatgtaaaaa taatccccgt tgtacacatt tataaataga tggccaatac ctcaaggcct
     3181 atggagttgg aatgttaatg acccgggagc aagcaataga aaacctgacg gcatacgatt
     3241 agtaccgtca gttaatacag gaacttataa tcgcaatgga ttttcaatac atagttgttt
     3301 aaacgctttt ggtccgtctt taggacctcg attctgttcc gagggttgta taactggttt
     3361 atctaatgac atgcaaaaat taaatgagtt aatattttca gagccagata gcacattaac
     3421 agttacggac taaaaaatat ggcaaggtta ttaacaattg tatttttttc tctatttctg
     3481 cgagtgcagt tactgcaaga caatttcaga gagagttaga tttggcagac aaagaaatat
     3541 tatgggcact aatatcagcc agtactaaag agggacgaaa agcgtgttca ctaagctatt
     3601 ttgcctgtaa ggccgcagag gctgaactcg gactggcata tatggctgcg aatgataaca
     3661 aggaatttct cacatcctta tctaatataa tgaggtacaa aatagatgct ggactttccg
     3721 aatcctatac atgctattta ttaagtaagg gaaagatcat aagaccatat ctgaaaaatc
     3781 taaatccact ccaacttgcc gctgattgca ttgaaacagt aaataaaata aaagataaga
     3841 ataaaaaaat cattgatata aattcagtta atatttgtag tgatgataaa
//
LOCUS       DAOFQD010000237         3722 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.241, whole genome shotgun sequence.
ACCESSION   DAOFQD010000237 DAOFQD010000000
VERSION     DAOFQD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.241"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            101..1339
                     /gene="alaC"
                     /locus_tag="QBF46_004706"
     CDS             101..1339
                     /gene="alaC"
                     /locus_tag="QBF46_004706"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDQ6676788.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHKNAQ"
     gene            complement(1404..1475)
                     /gene="ypdK"
                     /locus_tag="QBF46_004707"
     CDS             complement(1404..1475)
                     /gene="ypdK"
                     /locus_tag="QBF46_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDQ6676789.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1831..2751)
                     /gene="lpxP"
                     /locus_tag="QBF46_004708"
     CDS             complement(1831..2751)
                     /gene="lpxP"
                     /locus_tag="QBF46_004708"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDQ6676790.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3104..3346
                     /locus_tag="QBF46_004709"
     CDS             3104..3346
                     /locus_tag="QBF46_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDQ6676791.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FNMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3423..3698)
                     /gene="ypdI"
                     /locus_tag="QBF46_004710"
     CDS             complement(3423..3698)
                     /gene="ypdI"
                     /locus_tag="QBF46_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HDQ6676792.1"
                     /translation="MKVNLMLFSLFLLVSIMACNVFAFSISGGGSEASYKETEKTSAM
                     TTTHSTKFQPSQAILLKMREDAPPLNLTEEIPPPFPTKANYLIHPVR"
BASE COUNT          944 a          885 c          944 g          949 t
ORIGIN      
        1 cgctggacag acagaaatta atcaggctat tttcaaaaca gccaccatcg tggcagcgtc
       61 gctcggacgg tccgggcgct aacgttaatc tgaggaaatt atggctgaca ctcgccctga
      121 acgtcgcttt acgcgcattg atcgtctccc gccctatgtt tttaacatta ccgctgaact
      181 gaaaatggct gcgcgtcggc gcggcgaaga tattatcgat ttcagtatgg gtaacccgga
      241 cggtgcgact ccgccgcata tcgtcgaaaa attatgtact gtggcccagc gcccggacac
      301 gcatggttac tccacttcac gcggcattcc gcggctacgt cgcgccattt cccgttggta
      361 tcaggatcgc tacgacgttg aaatcgaccc ggaatcagaa gccatcgtca ccattggttc
      421 gaaagagggg ctggcacacc tgatgttggc gacgctggat cacggtgaca cagtgctggt
      481 gccgaatcca agttacccca ttcatattta cggcgcggtg attgccgggg ctcaggtacg
      541 ctcagtgccg ctggtggaag gtgtcgattt cttcaacgaa ctggagcgcg ccattcgtga
      601 aagttatccg aaaccgaaga tgatgatcct cggcttcccg tcgaacccaa ccgcgcaatg
      661 cgtggaactg gagttcttcg aaaaggttgt ggcactggcg aaacgctacg atgtgctggt
      721 ggtccatgac ctggcctatg ccgatatcgt ctacgatggc tggaaagcgc cgtcaatcat
      781 gcaggtgccg ggtgcacgcg atgtggcggt cgagttcttt acgctgtcga aaagctacaa
      841 catggcgggc tggcgtatcg gctttatggt tggcaacaaa acgctggtca gcgccctggc
      901 acgtattaaa agctatcacg attacggcac ctttacgccg ttgcaggtgg cggcgattgc
      961 ggcactcgag ggcgatcaac agtgcgtgcg cgacattgcc gaacagtaca aacgacgccg
     1021 tgatgtattg gttaaagggc tgcatgaagc gggctggatg gtcgaaatgc cgaaggcttc
     1081 gatgtatgtc tgggcgaaaa tcccggaacc ttacgctgcc atgggctcgc tggaatttgc
     1141 caaaaagctg cttaacgaag cgaaggtctg tgtctctcca gggattggct ttggcgacta
     1201 cggcgacacg catgtgcgct ttgcactgat tgaaaaccgc gaccgtattc gtcaggcgat
     1261 tcgtgggatt aaagcgatgt tccgtgccga cggtttacta cccgccagca gcaaacatat
     1321 tcacaaaaac gcgcaataaa caccgtcgtc agaaatgcaa acaggagcct gatggctcct
     1381 gtttttcatt gcacagtttg cttttagatc atcagggcaa aagtaccggc ccaaacgatg
     1441 accataaaag aaatgcccat aaagaaatat ttcactttac atctctccgc gtaattactg
     1501 ttacgcctga atgacttcat cacacctgat acagtgagtt gacaccgggt aaaacgacct
     1561 taaaagtcct gtattttacc gtgggcatca ccccagaatt ctgagctaaa ctgctcgaga
     1621 cggcgctaat gtacgcctaa aactgaaacg agacaagagg cattttctta tttactttta
     1681 gtaacttaca ggtgtctcat atcggcgaca gtttaatttc tgacggtaat aattagatct
     1741 tcagcgaagg aaaaaacctt aagaaaaaag ccataaaaac catgaggtta ttatggctga
     1801 tttgaggagg gaaagagtga gagcagtttg ttaaatgtac aacgacgatt ctcccaccgg
     1861 gcgcgtttta aagcgacggt ggatccagag gtactgctcc ggtgcgcgca tgatctcttt
     1921 ctcgataatc ttgttcatat aggcagcggc ttgattttca tctgccgggt agccttccat
     1981 ctctggggtg atgtacaaac gatatccgct gtaatccgct tttcttacca tcgttacggt
     2041 caacatggct gcgccagaga gacgagagag gacataggtg ccattggttg tggcgacatt
     2101 ttccaccgca aagaacggcg cgaaggagct gcctttacga ccataatcct gatcgggagc
     2161 aaaccatacc gcttcacctt tcttcagtgc accgacaatg ccgcgcagat tatttctgcc
     2221 gatcatcgct ttgttagagc gcatgcgccc acgggtctgc acccattcca tcagctgatt
     2281 attatgtgga cgataggtag ccatcattgg ctgacaaagt cccatcacgc ggccgcccag
     2341 ttccagcgac ataaaatgga cgccgacaac cattacgccg cgattttgca tttgtgcgcg
     2401 tttaaggtta tccagccctt caacatcaaa ccatttacgt acgcgactgt cgggccagaa
     2461 ccatgccatg ccagtttcta ccagcgccat gccgagtgaa cgaaagtttt cggcaatcat
     2521 cttctcgcgc tcttcagcag aatgctgcgg gaaacaaagt tccaggtttt tacgggcgat
     2581 agattcacgc cgtttcagga atggtcgcgc cattgcgcca atacgcgtgc cgagaaagca
     2641 gagaacagga taaggaagct gtacccaaag ccagagtaca ccaagcccaa accatgtgag
     2701 ccagtagcga ggatgcagaa actcgcggga aaatttgcat tgtggaaaca tagttacctt
     2761 ctgtagatac gaattgttgt atcgcagaag attccatgtc gacagcggta cactcaattg
     2821 gttgggccat catgtacgac aatggttcct gtaaagagtg taaagactag tcaggacata
     2881 accacgagga gcatgaatgc ggtatctgta ctttatcact cgctgactgc tttcagctac
     2941 tgattattaa tcgtgcaaag taaaaagttt tccccacgca atagtgttgc atttaacgtg
     3001 ctgttttcat tgatgcttag caaaaattgc cagaggttaa ggattgtctt actattaaag
     3061 tgactgaagt tacaggtttt ttagcgcggc aggaggttga gctatgattt atttatggat
     3121 gtttctcgcc ctgtgtattg tctgcgtaag cggatacatc ggtcaggtac tgaacgtggt
     3181 atcggcggtc tcttctttct tcggcatggt gatcctcgct gcactcattt attacttcaa
     3241 catgtggtta actggcggta atgaactggt gacggggata tttatgtttc ttgccccggc
     3301 ttgtggcttg atgattcgct ttatggtggg gtatggcagg cgatagtggg gcaggatggt
     3361 tcatttgggc aggtgttcac atcagcatca gacatgattg ttcacctgcc atgtgacaga
     3421 cgctatcgca caggatgaat aagataattc gcctttgtcg gaaaaggggg cggaatttct
     3481 tctgtgaggt ttaatggtgg cgcatcttct ctcattttta acaaaatcgc ctgtgatggc
     3541 tgaaattttg tagagtgagt tgtcgtcatc gctgacgttt tttccgtctc tttatagctc
     3601 gcctcactac caccaccaga aatagaaaat gcaaaaacat tgcatgccat aatagagacc
     3661 aataaaaata agctaaaaag cattaagttt actttcatag aaagttatcc tgaaaatggt
     3721 tc
//
LOCUS       DAOFQD010000238         3627 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.242, whole genome shotgun sequence.
ACCESSION   DAOFQD010000238 DAOFQD010000000
VERSION     DAOFQD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.242"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..984)
                     /locus_tag="QBF46_004711"
     CDS             complement(<1..984)
                     /locus_tag="QBF46_004711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6676793.1"
                     /translation="MDVTLCLPALYPLRYVVDHLEYRSPSTQSASLQAIKFFYDFWFM
                     KHRTTFCYSFYFSGHDPAIAIHEMADFFQYLENGRIVNSTPRSLPFKHSSSVTNAARV
                     RAVIRFIGYLIATYISPYYRNETPKELSRHASRLNTRLLICKEEFKTLERSNHRYYRV
                     IPGFRSMTGDMVENVYRIVVPNSKHKNNFLNPFPSGFIQFRNYLILRLMLNYGLRVAE
                     LLLLECTSVKASIKGDKFSLIISMPSNITDPRIHAPSLKNDYSHRVLELDKLDYEFLM
                     IYMGKIRKSSLTHNFIFASSQNPDKPLSYNAVHLIFSEIDKVFSHYYPECKS"
     gene            1327..2727
                     /locus_tag="QBF46_004712"
     CDS             1327..2727
                     /locus_tag="QBF46_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDQ6676794.1"
                     /translation="MAINDAGMFTVKEINRLKILQDVIDRNIRPGQAAEMLGITPRHC
                     SRLLKRYRQYGPLGMNNQSRGRTGNRLLPASLTDQALSIIREHYRDFGPTLAREKLEE
                     VHGLVLGKETIRRLMIKAGLWIPRRQRAPKIHQPRYRRPCTGELIQIDGCDHHWFENR
                     GRPSTALVYVDDATSRLMHLLFVKSESTFTYFEATRGYIEKYGKPMILYSDKASVFRV
                     NNKHATTGPGETQFARAMRCLNITPLCAETSQAKGRVERAHLTLQDRLVKELRLKGIC
                     TIEAANAFAEEYMADYNRRFAKEPLHDFNAHRPLALDDDLDAEFTWREPRRVSKNLTV
                     QYDKMLYLIEDNEYSRRAIGKYIDVWHYPDGHKELRLNGVLLPYSTYDRLSEVDPVAI
                     VDNKRLGHVLDVARQVQRKRDNNRSQSLPCSGDEPSRRRHAPSINKSQRSLNEDDLLE
                     AMIKLQGSSEAIFGKR"
     gene            2962..>3627
                     /locus_tag="QBF46_004713"
     CDS             2962..>3627
                     /locus_tag="QBF46_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676795.1"
                     /translation="MAIINIYSKRQRKIRGEVNDVYQYNNIPHALRVQIIKIITDSIG
                     FPSSNERYTSYRNEADKVYAYIHEILSKEYGVFSLKEFAKNDFDALVDFFLKERNTEK
                     CLDFIEICFQILVSHVAKNHYEFKDITSQSPGDAVIELNERFREHGVGYQFESEEIIR
                     VDSQLIHADVVKPTLILLSGEPLFEGANDEFLAAHEHYRHKRYKECLNDCLKSFESIM
                     KAIH"
BASE COUNT         1116 a          762 c          796 g          953 t
ORIGIN      
        1 agacttacat tcaggatagt aatgtgaaaa aaccttatct atctcagaaa aaattaaatg
       61 cactgcatta taggacaacg gtttatcagg gttttggctc gatgcaaata taaagttgtg
      121 agtcagcgag gattttctaa tttttcccat ataaatcata agaaattcat aatcaagctt
      181 gtcaagctcc aaaactcgat gtgagtaatc attttttaat gacggggcat gtattcttgg
      241 atcagttatg ttgctaggca tgctaatgat taagctaaat ttatcacctt ttatactagc
      301 tttaaccgat gtgcactcta ataataacag ctcagcgaca cgcagcccat agttgagcat
      361 taaccgcaaa atcaaataat tacgaaactg gataaaccca gaagggaagg ggtttagaaa
      421 gttattttta tgttttgaat ttggtactac tatacgataa acgttttcaa ccatatcacc
      481 agtcatactg cgaaatcccg gaatgacgcg ataataccga tgattacttc tttcaagagt
      541 tttaaattcc tctttacata tcagcaaacg agtattcagg cgggatgcat gacgagacaa
      601 ttctttaggt gtttcatttc gatagtaagg agatatatag gtagcgatta ggtagcctat
      661 gaatctaatc actgcacgta ctctggcggc atttgtcacg ctgctagaat gtttaaatgg
      721 cagtgatcgt ggtgtggagt ttactatccg accattttct aaatattgaa aaaagtcagc
      781 catctcatgt atagcgattg cagggtcatg tccggagaaa taaaagctat agcagaatgt
      841 agttctatgc ttcatgaacc aaaaatcata aaaaaactta attgcctgga gggaggctga
      901 ttgagttgag ggcgagcgat actccagatg gtcaactaca taccttaacg gataaagtgc
      961 gggtaaacag agggttacat ccatcagaat ccagcgccgt cggccatttg aacttacaat
     1021 ttgggaaagt gagtacatag atacacccaa catgcagaat gaggtgaagt aaatgtacga
     1081 tagacctaac gcaataaaag ggaggtatgt caaaattccc aacatgttga tttttgtagc
     1141 tgaaccagtg aatcatgaag taatagaccc atggaggtta tttaacataa tatacattat
     1201 gcgcaccaac attgtagagg ctcaatgcta atgtctattt gtggctcatt aggaatgtcc
     1261 aggctatagt ttctcagatc cctatgtcta gacaacgact cactttttcc tgatgaggag
     1321 ttttctatgg ctataaatga cgccggaatg ttcaccgtga aagaaatcaa tcgcctcaaa
     1381 atccttcagg atgtcatcga tcgcaatatc cgtcctggac aggctgccga aatgctgggc
     1441 atcacgccac gtcattgcag ccgtctgctc aaacgttatc gccagtatgg gccgcttggc
     1501 atgaacaacc agagtcgtgg ccgcactggt aaccgtctgt tgccagcgtc actgacagat
     1561 caggcactca gtataatccg ggagcactac cgtgattttg gccccacgct agcgcgggaa
     1621 aagctggagg aagttcacgg tctggtcctt ggaaaggaaa ccatccgacg tctcatgata
     1681 aaggccggac tctggattcc ccgcagacaa cgcgcaccga aaattcatca gcctcgttac
     1741 cggcgtcctt gtactggcga gctgatacaa attgatggct gcgaccacca ctggtttgaa
     1801 aaccggggtc gaccctctac tgccctggtc tatgttgacg atgccaccag tcgcctgatg
     1861 cacctgctgt ttgtgaaatc tgagtccaca tttacctatt ttgaagctac acgtggctat
     1921 atcgagaaat acggtaaacc gatgatcctc tacagcgata aagccagcgt attcagagtc
     1981 aataataagc acgccaccac cggccccggc gaaacccagt ttgccagggc catgcgctgc
     2041 cttaatatta ctcctttgtg tgctgaaacc agccaggcaa aaggccgcgt tgaacgtgct
     2101 catttgacac ttcaggaccg actcgttaag gagctcaggc ttaaggggat ctgtacaata
     2161 gaagcggcca atgcatttgc agaagaatac atggcggatt ataaccggcg ctttgccaaa
     2221 gaacccttgc atgattttaa cgcgcaccgg ccgctggcat tagacgatga ccttgatgca
     2281 gaatttacct ggcgagagcc tcgccgggta tcaaaaaatc tgactgtaca atacgataaa
     2341 atgctctatc tgattgagga caacgagtac agtcgaagag ccatcggcaa gtatattgat
     2401 gtctggcatt atcctgacgg acataaggag ctgcgactaa atggcgtgct gctgccctac
     2461 tccacctatg accggctttc tgaggttgat cccgtcgcca ttgtagataa caagagactt
     2521 ggccatgtgt tggatgttgc ccgtcaggtc cagagaaaac gggacaataa tcgctcacaa
     2581 tcgttacctt gttctggtga tgagccttcc cgcagacggc acgcccctag catcaacaaa
     2641 tctcagcgct cactgaacga agatgaccta cttgaagcca tgataaagct tcagggaagc
     2701 tctgaggcca tctttggtaa aaggtgaact tggcggaaac ctcgtactaa ctgaccaacg
     2761 gcgatgtgtg cgctatcttt cggctattgc aatcaaagcc agagaagatt tctgagaaat
     2821 gcaaataact tcaatgatat tatatacaaa ggaaaccgag gtaaaattcg ggtgtcacag
     2881 aaccccgaac cgcttcgcgg tgcaaagagc caaacagcaa gtatcacaga acagcatgta
     2941 aaaaaatttt gaggctattt catggcaatt attaatattt attctaagag acaaagaaaa
     3001 attcgtggcg aagtaaatga cgtatatcaa tataacaata ttccacatgc actcagggtg
     3061 caaataatta aaataataac tgattctatc ggcttcccct cttctaatga acgctacaca
     3121 tcgtatagaa atgaagctga caaagtatat gcatatatcc atgagatact ttctaaggaa
     3181 tatggtgttt tttctttgaa ggaatttgca aagaatgact ttgacgctct tgtcgacttt
     3241 ttccttaaag agagaaatac agagaaatgc ctagatttca tagaaatatg cttccagata
     3301 ctagtgagcc acgttgcgaa aaaccattat gaattcaaag acataacaag tcaaagtcct
     3361 ggagatgcag ttattgaact aaatgaaagg tttagagaac atggggttgg ttatcagttt
     3421 gaatctgaag agataataag ggtagactct caattgattc atgctgatgt cgtaaagcca
     3481 accctgattc ttttaagtgg agaacccttg tttgaaggcg ccaatgatga attccttgct
     3541 gcgcatgagc attacaggca taagcgttat aaagaatgtc taaatgattg tctgaaatca
     3601 tttgaaagta taatgaaagc aatacat
//
LOCUS       DAOFQD010000239         3458 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.243, whole genome shotgun sequence.
ACCESSION   DAOFQD010000239 DAOFQD010000000
VERSION     DAOFQD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.243"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            83..198
                     /gene="rrf"
                     /locus_tag="QBF46_004714"
     rRNA            83..198
                     /gene="rrf"
                     /locus_tag="QBF46_004714"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(302..829)
                     /gene="mobB"
                     /locus_tag="QBF46_004715"
     CDS             complement(302..829)
                     /gene="mobB"
                     /locus_tag="QBF46_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDQ6676796.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(811..1395)
                     /gene="mobA"
                     /locus_tag="QBF46_004716"
     CDS             complement(811..1395)
                     /gene="mobA"
                     /locus_tag="QBF46_004716"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDQ6676797.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1465..1734
                     /locus_tag="QBF46_004717"
     CDS             1465..1734
                     /locus_tag="QBF46_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDQ6676798.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1811..2797
                     /gene="srkA"
                     /locus_tag="QBF46_004718"
     CDS             1811..2797
                     /gene="srkA"
                     /locus_tag="QBF46_004718"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDQ6676799.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEELSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2814..3440
                     /gene="dsbA"
                     /locus_tag="QBF46_004719"
     CDS             2814..3440
                     /gene="dsbA"
                     /locus_tag="QBF46_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDQ6676800.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          882 a          834 c          856 g          886 t
ORIGIN      
        1 tgttttggcg gatgagagaa agattttcag cctgatacag attaaatcag aacgcagaag
       61 cggtctgata aaacagaatt tgcctggcgg ccttagcgcg gtggtcccac ctgaccccat
      121 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
      181 agtagggaac tgccaggcat caaattaagc agaaggccat ccgtcaggat ggcctttttg
      241 cattggcgca gaaaaaaatg ccggatgcga cgctggcgcg tcttatccag cctactcttg
      301 cttatccgtt ctgcttttgc atccactcca ccacaaaatc agccagcccc tcaacatcat
      361 taatatccag taatgcgaca tcaagattaa gcggcacatc actggctaca gcaataacat
      421 gcctgtctat cactaattct tcaggtcgat gtccggctcc atcgcgaaac agcacaatct
      481 ttgcgatctc ttcatgctta aacccttcga ccagaatcaa atccagcttt gaggtatcca
      541 ttcgacttgc gagaaactgt agatccagct cttcttcgtc tggtgtttcc gtcatcaagg
      601 cccatcgttg ctggctggca acgatggttt gtgccgcgcc agccttgcgc agctcatagc
      661 tatctttgcc tggcttatca acatccatat catggtgcgt atgcttaatc agccctggac
      721 ggatccctct ggcgcataat gccgggatca gttttttcaa aagcgtagtt tttccggtac
      781 cactccacgc ggcaaaggcg agtaacggta tcatcgtttt tcctgccatc gggcaagctc
      841 ctctggcgta ttcacgttaa caaatgcatc tttatgatcg ctgaaatcaa ccgcatgacc
      901 gccagccaga cgcataaatg ccattacccg gcgttctcct gcttgcagat attccagtaa
      961 taaaggctca atagcgcggt ttaccagagc aatagtcggg tgatcgcgtt caccgtcatg
     1021 gacccacacg acaggcgcat ctttgcgctg atgattaagc ctggctgcta aatcaggggg
     1081 aatgtaaggc gtatcgcacg ggcaaaacaa aaaccactca ccagcttcct gctgcattac
     1141 tgaaagcatt cctgccagag ggcctgggta atccgccagt gaatcttcaa tcactttcag
     1201 accgcttgcc tgatagattt cctgatgacg attagcatta accacgacgt gagagagctg
     1261 cgtcataagc gcgtcagcga catgttgcca cagcggtttg ccatttaagt ccagcaatcc
     1321 tttatctacg ccgcccattc gtctggcttt accgcctgcc agcacaacgc ctgttatcgt
     1381 cgtcatcaga ttcaccgata tcgcctcttt tattgtggga ttgaccctgc taacgtgtct
     1441 gtctcaagag taaggagcat cactatgaaa tgtaaacgtc tgaatgaagt tattgaactc
     1501 ctccagccag cctggcaaaa agagccagac cttaacctgt tgcaattttt gcagaaactg
     1561 gcgaaagagt caggttttga cggcgaactg gcggatttga cggatgacat tctgatctat
     1621 cacctgaaaa tgcgtgattc cgcgaaagat gcggtgatcc cgggtttgca gaaagattat
     1681 gaagaagact tcaaaacggc gctgttacgc gctcgcggcg taattaaaga gtaaaagctt
     1741 gtaagcggcg ccaccaaaat catcgtgaaa tgatatcctt catcattcgt aatgttttcc
     1801 ggatgatggg atgaacaaca gcgcttttac tttccagaca ctacacccgg ataccatcat
     1861 ggacgctctg tttgagcaag ggatccgggt ggattccggt cttaccccgc ttaacagcta
     1921 tgaaaaccgt gtctatcaat ttcaggacga agatcgtcga cgttttgtcg tcaaatttta
     1981 tcgccctgaa cgttggacag ccgatcaaat cctcgaagaa catcaatttg cgttgcagct
     2041 ggtaaatgat gaagttccgg tcgcagcacc tgtggccttt aacggtcaga ctttattgaa
     2101 tcatcaggga ttttatttcg ctgtttttcc aagcgtcggt ggtcgccagt tcgaagctga
     2161 taatatcgat cagatggaag cggttgggcg ttatttaggg cgtatgcacc agacggggcg
     2221 caaacagctt tttatccatc gcccgaccat cggtctgaat gaatatctca ttgagccacg
     2281 caagctgttt gaggacgcta cactgatacc ttccgggttg aaagcggcat tcctgaaagc
     2341 gacagatgag ctgatcgccg ccgttacagc acactggcgg gaagatttca ccgttctgcg
     2401 gctacatgga gactgccacg ccgggaatat tctctggcgc gatggtccaa tgtttgttga
     2461 tctggatgat gcacgtaatg gtccagctat tcaggatttg tggatgttgc tcaatggcga
     2521 taaagccgag cagcggatgc aactggaaac tattattgaa gcttatgaag agcttagcga
     2581 gttcgacacc gctgaaatcg gactgattga acctttacgc gccatgcgtt tggtttatta
     2641 tcttgcctgg ctaatgcggc gttgggctga tcccgcgttc ccgaaaaatt tcccgtggtt
     2701 aaccggggaa gattactggc tgcgacagac ggcgactttt atagaacagg caaaagttct
     2761 acaagaaccc cctttgcaat taacacctat gtattaatcg gagagagtag atcatgaaaa
     2821 agatttggct ggcgctggct ggtttagttt tagcgtttag cgcatcggcg gcgcagtatg
     2881 aagatggtaa acagtacact accctggaaa aaccggtagc tggcgcgccg caagtgctgg
     2941 agtttttctc tttcttctgc ccgcactgct atcagtttga agaagttctg catatttctg
     3001 ataacgtgaa gaaaaaactg ccggaaggcg tgaagatgac taaataccac gtcaacttca
     3061 tggggggtga cctgggcaaa gatctgactc aggcatgggc tgtggcgatg gcgctgggcg
     3121 tggaagacaa agtgactgtt ccactgtttg aaggcgtaca gaaaacccag accattcgtt
     3181 cagcatctga tatccgcgat gtatttatca atgcaggtat taaaggtgaa gagtacgacg
     3241 cggcgtggaa cagcttcgtg gtgaaatctc tggtcgctca gcaggaaaaa gccgcagctg
     3301 atgtgcaatt gcgtggtgtt ccggcgatgt ttgttaacgg taaatatcag ctgaatccgc
     3361 agggtatgga taccagcaat atggatgttt ttgttcagca gtatgctgat acggtgaaat
     3421 atctgtccga gaaaaaataa ttcaatgtaa attacata
//
LOCUS       DAOFQD010000240         3415 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.244, whole genome shotgun sequence.
ACCESSION   DAOFQD010000240 DAOFQD010000000
VERSION     DAOFQD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.244"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(175..1437)
                     /locus_tag="QBF46_004720"
     CDS             complement(175..1437)
                     /locus_tag="QBF46_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDQ6676801.1"
                     /translation="MSLNDSKIRKLKPSSRPVKLSDAHGLYLLVNPGGSRIWYLKYRF
                     NGKESRVSLGAYPLVSLAEARQQRDGIRKLLAQNINPAQQRMAEKSACSPEKCFKAVA
                     LAWHKTNKKWSADYAARILASMENHIFPAVGHLPVAALKTQDFTALLRVIENKGFLEV
                     ASRTRQQLSNIMRYAVQQGLTDSNPAQHLEGVTASPVKNHYPALPLERLPELLDRIGD
                     YRQGRELTRLAVVLTLHLFIRSSELRFARWSEIDFRHKIWTIPATREAIDKVRFSGRG
                     AKMRTPHIVPLSRQAIAILKQIQELSGHLDLVFPGDHNPYKPMSENTTNRALRLMGYD
                     TKTEICGHGLRAMACSVLVESDLWSRDTVERQMSHQERNSVRAAYVHKAEHLEARKAM
                     MQWWSDYLDVCREGYVAPYIYARQHKAA"
     gene            complement(1599..1674)
                     /locus_tag="QBF46_004721"
     tRNA            complement(1599..1674)
                     /locus_tag="QBF46_004721"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1639..1641),aa:Asn,seq:gtt)
     gene            1679..3322
                     /gene="yeeO"
                     /locus_tag="QBF46_004722"
     CDS             1679..3322
                     /gene="yeeO"
                     /locus_tag="QBF46_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDQ6676802.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT          766 a          796 c          907 g          946 t
ORIGIN      
        1 cgataagtag ttcattgatt ggttttcacc caaaattatt tggggccaaa tatttataga
       61 tgttttccga ttgataataa caaaaaaaac ggcaatatct atcaacaaaa aaaagcagat
      121 cccggtcaat caggatctgc ttttccagtt ttaatgattt acgctatttc tgattcaggc
      181 tgccttatgc tgccgcgcat aaatgtacgg cgcgacatac ccctcgcggc acacatccag
      241 ataatccgac caccactgca tcatggcctt tctggcctcc agatgctccg ccttatgcac
      301 atatgccgca cgcacgctgt tgcgctcctg gtggctcatt tgccgctcca ctgtatcgcg
      361 tgaccacaga tccgactcca ccaggacgct acaggccatt gccctgagtc catgcccgca
      421 gatttcagtt ttcgtatcat accccatcag acgcagcgcc cggttggtgg tgttttcgct
      481 cattggcttg tacggattat ggtcgccggg aaacaccaga tccagatggc cggaaagctc
      541 ctgtatctgt ttcagaatgg caatcgcctg gcgggagagc ggtacgatat gcggggtgcg
      601 catttttgcg ccacgccccg aaaaccgtac tttatcaatg gcctcgcggg ttgcggggat
      661 ggtccagatt ttgtgcctga aatcaatctc actccagcgg gcgaaacgca gttcgctgga
      721 acggatgaac aggtgcaacg tcagcaccac cgccagccgg gttaactccc ggccctgccg
      781 gtagtcgcca atgcggtcaa gcagttcagg caatcgctcc agcggtaaag cgggatagtg
      841 attcttgacg ggggaggcag ttacaccttc cagatgctgc gccggattac tgtcggtaag
      901 tccctgctga acggcatagc gcatgatgtt gctgagttgc tgccgggttc gggacgcgac
      961 ttccagaaag cctttattct cgataacccg caacaaagcc gtgaaatcct gcgttttaag
     1021 cgcagcaacg ggcaggtgac caaccgccgg gaagatatgg ttttccatac tggcgagaat
     1081 acgggcggca taatcagccg accatttttt gttggttttg tgccaggcca gcgccaccgc
     1141 cttaaaacac ttttcagggg agcaggcgga tttctctgcc atgcgctgtt gcgccgggtt
     1201 gatattctgc gccagtagct tgcggatacc gtcgcgctgt tgcctggctt ctgccagtga
     1261 gaccagcggg tatgcgccaa gactgactct ggattctttt ccgttgaaac gatatttgag
     1321 ataccagatg cgtgaaccgc ccggattgac gagcagatac agaccgtgag cgtcggagag
     1381 ttttaccgga cgggaagaag gttttaactt gcggatttta gaatcgttta aagacatttg
     1441 ggggtcactc cagaatcgaa ccaaactgac cccagatctg accaccaaat tatcccgatg
     1501 cggagagaaa accagatacg caccgggaag gattttcacg ccaacttact gaatcgtaaa
     1561 caaatagaga cgtacagata agcatgaaaa cttgaatttg gctcctctga ctggactcga
     1621 accagtgaca tacggattaa cagtccgccg ttctaccgac tgaactacag aggaatcgct
     1681 gttgaggcac atcttaacgg cgaaaaatct tttgtcaaac ccgattttta aattccccaa
     1741 ctgtttgccg tttctatcaa cagtttgttg catttgcaga caatttgttg gcgaaaatct
     1801 ttgcagcttt gctgattctc cctcattatt tgaaatgtgg tttcactttc tgcaattaag
     1861 gtcggctttg aatatctcct ctgctttacg ccaggttgtt cacggcactc gctggcacgc
     1921 taaacgcaag agctacaaag tgttgttctg gcgcgagata accccgcttg ctgttcctat
     1981 cttcatggag aatgcctgtg tcctgttgat gggggttctg agcacttttc tggtcagctg
     2041 gctgggaaaa gatgcgatgg ccggcgtggg attggcggac agcttcaata tggtcattat
     2101 ggcttttttt gctgctatcg atcttggtac tactgtcgtt gtggcattta gtctcggtaa
     2161 gcgggatcga cgacgagcga gggtggcgac gcggcagtca ttggtgatca tgacgttgtt
     2221 tgccgtactg ttggcaacgc ttattcatca ttttggcgaa caaattattg atttcgtcgc
     2281 gggtgatgcc acgacagaag ttaaagcact ggcgttgact tatctggagc tgacggtact
     2341 cagttatcca gcagctgcca tcactttgat tggtagcggg gcacttcgtg gtgcagggaa
     2401 tacgaaaata ccgctactga ttaacggtag cttgaatatt cttaatatta ttattagcgg
     2461 catattgatt tacggccttt tctcctggcc gggactggga tttgtcgggg ctgggttggg
     2521 tttaaccatt tctcgttata ttggcgcagt tgcaattttg tgggtgctgg cgattggttt
     2581 taatcctgcg ctaaggattt cgttaaagag ctattttaaa ccgctgaatt ttagcattat
     2641 ctgggaagtc atggggattg gtattccagc gagtgtcgaa tcagtgttat ttaccagtgg
     2701 tcggttatta acccaaatgt tcgttgccgg gatggggacc agtgttattg ccggaaattt
     2761 tatcgcgttt tcaattgcgg ctcttatcaa cttacccgga agtgcgctcg gctctgcttc
     2821 tacgatcatt acaggccgaa ggttgggggt agggcagata gcgcaagcag agattcagtt
     2881 gcggcatgtg ttctggcttt ccactcttgg attaacggcc atcgcctggc taacggctcc
     2941 ctttgccggg gttatggcat cgttttacac ccaggatcca caggttaaac atgtcgttgt
     3001 gattctgatt tggctaaatg ctttatttat gcctatttgg tccgcctcat gggtgctacc
     3061 cgctggattt aaaggtgctc gtgatgcccg ttacgccatg tgggtttcga tgttgagcat
     3121 gtggggttgt cgggttgtag tcggttatgt gctgggaatc atgcttggct ggggtgtggt
     3181 tggtgtctgg atgggaatgt ttgccgactg ggctgtgcgg gccgtgctgt tttactggcg
     3241 aatggttact ggacgttggc tatggaaata ccctcgaccc gagccgcaaa agtgtgaaaa
     3301 aaagccagtt gtgtcggaat aaacgacaaa atgcagatta tttcagcaaa cgatttcaaa
     3361 tttaaaaaac aggctttgac attgtgggtg ggcatcgcta atattcgcct cgttc
//
LOCUS       DAOFQD010000241         3404 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.245, whole genome shotgun sequence.
ACCESSION   DAOFQD010000241 DAOFQD010000000
VERSION     DAOFQD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.245"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(73..882)
                     /gene="murI"
                     /locus_tag="QBF46_004723"
     CDS             complement(73..882)
                     /gene="murI"
                     /locus_tag="QBF46_004723"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDQ6676803.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(916..2376)
                     /locus_tag="QBF46_004724"
     CDS             complement(916..2376)
                     /locus_tag="QBF46_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6676804.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            complement(2369..2647)
                     /locus_tag="QBF46_004725"
     CDS             complement(2369..2647)
                     /locus_tag="QBF46_004725"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676805.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVNVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            complement(2628..3113)
                     /locus_tag="QBF46_004726"
     CDS             complement(2628..3113)
                     /locus_tag="QBF46_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDQ6676806.1"
                     /translation="MKNKSGNSCYREAVNIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
BASE COUNT         1138 a          671 c          599 g          996 t
ORIGIN      
        1 ccttactcag ttggtgtcca acttttgggg tgcagttcag aaggacgggg ttttacggtg
       61 caccggataa atttaaaact gcctgatact gaatgtatga ggtttcgtaa tgctcaattc
      121 ccctagtacc gttttgtaga tctccatact tccactagta tttatttgca aagttcctat
      181 atttgaggag tttaataaat ttagctgtcc caagtgattt ttaatagaga tagcctgatc
      241 atgtgcagga ttaataaatt ttatatccgg tgcaactgct tcaaaaaaat tctgaacaat
      301 tggatagtga gtacagccga gaataatttc atttactgga tggagggaaa ttaaatggtt
      361 aacatgtttt ttgatgtcat tcaagattgc aggagcatta agattacctt cctcaattaa
      421 cattgcaaga tttttgcttg gttctccaaa tacatttatt gttgagttca atttttttat
      481 tagctctttg tgacaccccg ttttaatagt aaactcggta gcgataatac caacattttt
      541 atattgctgg cgaatcacat cttctactgc aggttcaatg atggataaga taggaaacgg
      601 aaagctctta gcatatccgc tgtattcttc ggattccaaa gttgaggata tagtattaca
      661 tgcgacagca actagtttga cattccttga ctgtaaaaaa gtcagcattt ttttagtcaa
      721 actgatgata tcatcggcag aacggttgcc ataaggtgca ttcatattat ctccacaata
      781 gataatatct tcaccaggaa gcaaattaat tatttccttt accgatgtca atccacctac
      841 acctgagtct attacaccaa ttggcgattc agcactaaac atatttaacc tcatcttttg
      901 tagcttatgg aaaatttaat tactagttct tttttcccaa gaaaatggtt cgaaatcaga
      961 gtttggatct ttccagtcag attttcggaa ggcgtaaatt aatattggaa ttaataaaaa
     1021 tagggcaact aatactaaca aataaagaac atagctggtt ggactaccag ttgggatttg
     1081 ctcgggcgga ataaaactta atacaaaagc cagaaaagac cctaagaatc caagtgttgc
     1141 ccagaaaaat aaagcagggg ctcgatatgg gcgttttgta tcaggttgag aatgtcgtag
     1201 atagatagca ctagcgaaca tgagcatata cataactaaa tagagcataa ctgttaactg
     1261 tgataaaatc tgaaaagctg cttgaactga aggcattatc acaaaagtaa cagaaagaac
     1321 tgagacaatt ataccttgta caaacattag atggtgcccc attccatgac gattggtttt
     1381 ttggaaaaac ttaggtaaat aaccagcttt cgcgatcgct aatacacctg tattaggccc
     1441 tgcaatccaa gtaaccaccc cacccagtac gccaattgct agcataaatg ccatcacaga
     1501 gccaagccat ggcactccag cccattcgaa cagcatatcg taggcaacca acaaactttg
     1561 agttagacta attttatctg taggaataat aaaggcgacc cctaaagtac tgagcaggaa
     1621 tattgcaata gtacctatag aagcgatggt aattgcaatt gggtattttt tagttggatt
     1681 atctaaatcg ttaacatgga ccgcattcat ttccatccct gcataagcaa gaaatatgct
     1741 ggcggcaaga acaatattct ggaagttagc tatattaggg aaaagatcac cccaacctaa
     1801 tgggatcacc gctggtttac cgctgaacca cagataggag aaaccaagaa taacaaggat
     1861 aatagctgga ataattgtcc ctataatacc gcaccactta gatacctgcg agaaaatacc
     1921 aacgccacgt aatgcaacac aaatagctcc ccagtaaaca aatagtacga aaaataaaat
     1981 ataccattta ttactggcaa ttgattctgc cacccccata tcaacatcaa taaatgcaac
     2041 cgagactgca ccaaatgtca gtactgttgg aaagtagggg atgacttgta gccaggtcat
     2101 aagcattcct acaaaacccc agcgagcccc aaatgcttct cctatccatc gaaatgcccc
     2161 ccctttttgt ggatatgttg tagctagctc agccgcaaca aaggcaacag gtactaaaaa
     2221 cataattgca gcaaaggtat agtaaaaaat ggcacctaag ccgtattctg cttcagaagg
     2281 tataaatcgt agacttacta cagcagttat attcatgata gcgagtgtcc ccactgtcat
     2341 tttcttactt ttgggtgcga tattagcatt agacattttt atactctcga atatttttaa
     2401 aatagaaaca tataattacg atgtaaatat aaaaactaat attaacttta acacttatct
     2461 taaaaacatt aacgcaatct gctttacaaa aataagtaat acctaaagtc gtaacgacac
     2521 caatagtgtt ttcatcgaca tgcttcaaaa cctctttagc attcattatc aggtgatgat
     2581 catcaagtgg aatttcacac aatttcacat cacagtaacg gcagaattta tgccagcata
     2641 tttgcacagg atcgcaaatc aggttcggtt gatttacctt gtacttcgcg ttttttacac
     2701 cactgccatt ttaacgccaa gcggccgagc atagcagctt cagatgaacc aatggttgag
     2761 caacctaagg tgtgtcgcga atcggggaaa ctccacgaat cggaaagcat acgcacacag
     2821 cgaccttcag gttccgccat ttgtggatat tcatctttat aaatcatgtt tttatcaata
     2881 gctaaattaa gcaactcacg gatttcatca tcaagccatg tttgacagaa agtagctaag
     2941 ttttgtcgtg aatccccatc aaggatgagc tcatcacgta tggcgctgaa tacatttcga
     3001 gatttcttct catctacacg caaatgatat ttatgcaata taaatgaaag ctcaagtgag
     3061 ctatgaatat tgactgcttc tctataacaa gaattacctg atttattttt cataataaac
     3121 gccactcaat acaatcagat taaacattac aaacacaatc tggattttgc aattttaaaa
     3181 ctatcaattt tagatatatt ttaattaata taaaaccata aacatatcaa aaaattactt
     3241 tattatggtc atggtagatg tgtttcaatg ccaacctggt ttattcctac atatgtgaga
     3301 gcatatttac aaaaaaatcg ttattgtgat ttcaacggtg atgctgaagt ggcacactaa
     3361 atttggccac ctgaacagag gtgatatgct cacctcagaa caac
//
LOCUS       DAOFQD010000242         3369 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.246, whole genome shotgun sequence.
ACCESSION   DAOFQD010000242 DAOFQD010000000
VERSION     DAOFQD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.246"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            112..2919
                     /locus_tag="QBF46_004727"
     CDS             112..2919
                     /locus_tag="QBF46_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014907318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDQ6676807.1"
                     /translation="MPVNPQIPEDDVLLKNLNNVQLKNQLPPSPVLDSHDFTVEMETG
                     TGKTYVYLRTMLELNKRYGMSKFIIVVPSVAIKEGVYKTLQITEEHFKSLYAGVPYEY
                     YLYDSAKPADVRNFATSSVMQIMVMTVGAINKKDVNKLYQSSEKTTVDDLDKPIDLIR
                     ATRPIIIVDEPQSVDGGLNGAGKTALDAMHPMFTLRYSATHVHKHHMVYRLDAIDAYN
                     QKLVKQIEVAGLEVQNASNSPYVKLVSINSKKNVISGTVLVDIADKKGNVDRSEITVY
                     DGMTLDDETGRDIYQGYRISEIRTGKEGYLTLETPSDTYYMKVGDVVGDIDPMLIQRH
                     MIRRTIREHLDKELRLNTQQIKVLTLFFIDKVDFYRQFTEEGQREPGEYARIFEEEYL
                     KAIKSPAYKDLLPSHPEHAKDVHDGYFSIDKKGAWAETSESNNAGRENAERGYNLIMK
                     NKEKLLSFSNPVRFIFSHSALKEGWDNPNVFQICSLREMGSERERRQTLGRGLRLCVN
                     QDGERVRDDSINILTVVASENYQAYAERLQSEIENETGIRFGIVEKDAFAHLICSDSS
                     QVVGIIASQEIWSELHAEGYLEKTGKITKTLKETLFDGTFTVSEKFLPLQEQIESILR
                     KSSGRLNINNADERKTIKYRKEILNSPEFEALWQRISQKTLYRLAFDEDSLIKSCTKS
                     LSEMQPVAKAMVSFSKATIKQSQSGLGVEVQSNGTTSSVIDVAEQPLPDILGVLQEKT
                     GLTRRSLSRILKESEKLADFAKNPQQFISEAITRINYCKRLSIVDGIKYYPLKGEVYA
                     QSLFMDKELTGYARNLFETSSKSVYEYVPKDSEVESRFAQELEEQDEVKLYVKLPGWF
                     KIPTPLGTYNPDWALVIEDNGEDHIYFVVETKSKHVGLGNEEDAKITCGNAHFMSLKD
                     QLINPARYMRSTNLNEVVSSI"
BASE COUNT         1048 a          696 c          710 g          915 t
ORIGIN      
        1 gccataactt cggtctgcga tctgtttgaa gggcaagaga aatgggaaag cgaaatgact
       61 gtgctcgcac cagctcaggg tgcactctca tttgaaggcg caacaggttc aatgcctgtt
      121 aacccacaaa tacccgaaga tgatgtactg cttaaaaatc tgaacaatgt tcagttaaaa
      181 aaccagcttc caccttcacc tgtgctcgac agccatgatt ttactgtcga aatggaaaca
      241 gggacaggta aaacgtatgt ttatcttcgc accatgctgg agctgaataa acgctatggc
      301 atgagtaaat ttattattgt ggttccttca gttgctatca aggaaggcgt ttataaaaca
      361 ctacaaatca ctgaagaaca tttcaaaagc ctgtatgcgg gagttccata cgaatactat
      421 ctgtatgatt cggcaaaacc tgcggatgtt cgaaattttg caaccagttc tgttatgcag
      481 atcatggtaa tgactgtggg tgcgattaac aaaaaagatg ttaacaagct ctatcagtcc
      541 agtgaaaaaa caactgtgga cgatctggat aagcccattg atttaatccg tgctacacgt
      601 ccaattatta ttgtcgatga accgcaaagt gttgatggtg gcttgaacgg tgcgggtaaa
      661 accgcacttg atgctatgca ccctatgttc accttacgct attcagcaac gcatgtgcat
      721 aaacatcata tggtgtaccg ccttgatgcc attgatgctt acaaccaaaa actggtcaaa
      781 cagatcgagg ttgccgggct tgaagtccag aatgcaagca acagccctta tgtgaaactg
      841 gtcagtatta atagtaagaa aaatgttatc agcggtactg ttcttgttga tatagccgat
      901 aaaaaaggga acgtagatcg ttcagagatt accgtatatg acggtatgac acttgatgac
      961 gaaacggggc gtgatattta tcagggatac aggataagcg agatccgaac gggcaaagag
     1021 ggttatctta cactggaaac accttctgat acctactata tgaaagtcgg tgatgtcgtg
     1081 ggtgatattg accctatgct catccagcgt catatgatca gacgtacaat ccgtgaacat
     1141 ctggataaag aacttcgcct gaatactcag caaatcaagg ttcttaccct gtttttcatc
     1201 gataaggttg atttctaccg tcagttcact gaagaaggac aacgtgagcc gggtgaatat
     1261 gcccgtatct ttgaggaaga ataccttaaa gccatcaaaa gtcctgccta taaagattta
     1321 ctcccttctc atccagaaca tgctaaagac gttcatgatg gttatttctc cattgataaa
     1381 aaaggtgcat gggctgaaac aagcgaaagc aataatgcag gccgcgaaaa tgcggaacgt
     1441 ggttataatc tcatcatgaa aaataaagag aagcttttaa gtttctcgaa tccggtgagg
     1501 tttatttttt cacactctgc tttgaaagaa ggctgggata atcccaatgt gttccagatc
     1561 tgtagccttc gagagatggg aagtgaacga gaacgccgcc agactttagg ccgtggtctt
     1621 cgcctgtgtg ttaaccaaga tggtgagcgt gtccgggatg acagcattaa cattctgacc
     1681 gtcgttgcca gtgaaaatta tcaagcctac gctgagcgct tacagtctga aattgagaac
     1741 gaaacaggca tccgttttgg catcgtcgaa aaagatgcgt ttgcccatct gatatgctct
     1801 gattcctcac aagttgtagg tatcatcgct tcacaggaga tatggtctga actacatgct
     1861 gaaggttatc tggaaaaaac cgggaaaatc accaaaacac tgaaagaaac tcttttcgat
     1921 ggcactttta ctgttagcga aaagttcctt ccccttcagg agcaaattga atctatcctg
     1981 cgtaaatcat caggacgact caatataaat aatgctgatg agcgtaaaac tatcaaatac
     2041 cggaaagaga ttttaaacag cccggaattc gaagcgttat ggcaacgcat tagtcaaaaa
     2101 acattgtatc gtctggcatt tgatgaagat tcattgatca aatcctgtac gaaatcactt
     2161 tctgaaatgc agcctgtagc aaaagcaatg gtcagtttca gtaaagccac cataaaacaa
     2221 agccagtcag gtcttggtgt tgaagttcag tcaaatggaa cgacaagctc tgtaatagat
     2281 gttgcagagc agccactgcc agatattctt ggtgttctgc aggaaaaaac tggactgaca
     2341 agacgcagtt tgagccgcat cctgaaagag agtgaaaaac ttgccgattt tgctaaaaac
     2401 ccacagcagt ttattagtga agctatcacc agaataaact actgcaagcg cttgtctatt
     2461 gttgatggta ttaaatatta tccactaaaa ggagaggtgt atgcgcaaag tctctttatg
     2521 gataaggagc taacaggata tgcccgcaat cttttcgaaa cctcgtctaa gtcagtttac
     2581 gaatatgttc ctaaagattc tgaagtcgaa agccgtttcg ctcaggaact cgaagaacag
     2641 gatgaagtta agctttatgt gaagctgccg ggatggttta aaatccccac accactagga
     2701 acttacaatc ctgactgggc tttggttatt gaagacaatg gtgaagacca tatttacttt
     2761 gttgttgaga caaaaagtaa acacgttggt cttggtaatg aagaagatgc gaaaataact
     2821 tgtggcaatg cgcattttat gtcactcaaa gatcagttga ttaatcctgc tagatacatg
     2881 cgctctacca atctaaatga ggttgtgagc agcatataaa agaagggggc caagcggccc
     2941 tttcttttta ttttttgaat taaaccaact cgatttccgt gcccttaccc gcagatagtg
     3001 gtgagtgatt atttcatgct caggtcgaga cctgttatca aaactctttc aattatagta
     3061 agtaagagtt aatctcttaa tttacaattc ttattgcaaa acatctaaca ttgtccactg
     3121 aacaatcatt cccgtcctca gtacaagcat cagacccact tgcgacccaa ccttgtgctg
     3181 ccattccatt actattaatg ttggattgac aaatatctcc ataattacta ccactagaaa
     3241 ctaaaaattg agttctctca attcttgcag aactactttg ccacaaacca ttaacacaag
     3301 ataaaatagc cccttctgac gttcttccct gtaatccatt aggagaacat ccccatccta
     3361 cattcgctt
//
LOCUS       DAOFQD010000243         3366 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.247, whole genome shotgun sequence.
ACCESSION   DAOFQD010000243 DAOFQD010000000
VERSION     DAOFQD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.247"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            74..1072
                     /gene="yiaK"
                     /locus_tag="QBF46_004728"
     CDS             74..1072
                     /gene="yiaK"
                     /locus_tag="QBF46_004728"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDQ6676808.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1084..1551
                     /locus_tag="QBF46_004729"
     CDS             1084..1551
                     /locus_tag="QBF46_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDQ6676809.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            1669..2142
                     /gene="yiaM"
                     /locus_tag="QBF46_004730"
     CDS             1669..2142
                     /gene="yiaM"
                     /locus_tag="QBF46_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDQ6676810.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            2145..>3366
                     /gene="yiaN"
                     /locus_tag="QBF46_004731"
     CDS             2145..>3366
                     /gene="yiaN"
                     /locus_tag="QBF46_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDQ6676811.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLL"
BASE COUNT          774 a          822 c          840 g          930 t
ORIGIN      
        1 tatatttgaa atcaagtttc gcatattgaa attttaagcc aaaaaagcga tcaaaaaaac
       61 aaggaagcct gggatgaaag tgacatttga gcagttaaaa gcagccttta atcgggtctt
      121 aatttcacgc ggcgttgaca gcgaaacggc tgacgcctgt gcagagatgt tcgcccgcac
      181 caccgaatcc ggcgtttatt ctcacggcgt taatcgtttc cctcgtttca ttcaacaact
      241 ggaaaacggc gatatcattc ctgatgccca acccaaacgt ataaccagcc tcggcgcaat
      301 tgaacagtgg gatgcccagc gttcgatcgg caacctgacg gcgaaaaaga tgatggatcg
      361 cgccattgaa ctggctgccg atcacggtat tggtctggtg gcacttcgta atgccaacca
      421 ctggatgcgc ggcggcagct acggctggca ggcggcggaa aaaggctata ttggcatttg
      481 ctggaccaac tccatcgccg taatgccgcc gtggggcgca aaagagtgtc gcattggcac
      541 taacccgctg atcgtcgcca ttccttccac gccgatcacc atggtcgata tgtcgatgtc
      601 gatgttctct tacggcatgt tagaagttaa ccgcctggcg ggccgtcagc tcccggtcga
      661 tggtggcttt gatgatgagg gcaatttgac caaagaacct ggcgttatcg agaagaatcg
      721 ccgcattttg ccgatgggct actggaaagg ttctggcatg tcgattgtgc tggatatgat
      781 cgctactctc ctttccgacg gcgcatcggt tgccgaagtc acccaggaca acagcgacga
      841 atacggcatt tcacaaattt ttattgccat tgaagtggac aagcttatcg acggtcccac
      901 ccgcgatgcc aagctgcaac gcatcatgga ttacgttact accgctgaac gcgctgacga
      961 aaaccaggcc atccgcttac ccggccacga atttactacc ctgctggccg aaaaccgccg
     1021 taacggtatt accgttgatg acagcgtgtg ggccaaaatc caggcgttat aaggagataa
     1081 gtcatgattt ttggacatat cgcgcagccc aatccgtgcc gtttgcccgc cgccattgaa
     1141 aaggcgctcg attttctgcg cgctaccgat ttcaacgccc tggagccggg cgttgtcgaa
     1201 atcgacggca aaaatattta tgcgcaaatt atcgatttaa ccacacgcga agcggtggaa
     1261 aaccgtccgg aagtgcatcg tcggtatatc gatattcagt ttctggcgtg gggtgaagag
     1321 aaaatcggca ttgctattga tacgggaaat aataaagtca gcgaatcact attagagcag
     1381 cgcgatatta ttttttatca tgacagtgaa catgaatcat ttatagaaat gataccgggc
     1441 agctacgcaa ttttctttcc gcaggatgtt caccgaccag gttgtatttt gcaaacagcc
     1501 tctgagatac gaaaaattgt ggttaaagtc gcattaacgg cgctgaatta atcgaataat
     1561 cgtctacgcc agaacgcctg ggtaatgtta ttgctctaac tatattaata attcaggttg
     1621 ctgcacatgc aacatgaatt atgacagaca tagcctcaag gaatagctat gaaaaaaata
     1681 ctcgaagcaa tactggcgat taatctcgcc gtactttcct gtattgtgtt tataaacatt
     1741 attttaagat atggttttca aacaagtatt ttatctgtag atgaattgtc gcgttattta
     1801 tttgtctggt taacatttat tggcgcgatt gtggctttta tggataacgc ccacgttcag
     1861 gtcacctttc tggtggagaa actctccccc gcatggcagc gacgagtcgc tctggttacc
     1921 cattccttaa tcttatttat ttgtggcgca ctggcctggg gcgcaacgct aaaaacaatt
     1981 caggactgga gcgattattc accgatcctc ggtttaccca tcggcctgat gtatgccgct
     2041 tgtctgccca ccagcctcgt tatcgcattc tttgaactgc gtcatttata tcaactcatc
     2101 acgcgtagca attctctaac gtcgccaccg caaggagctt aatcatggct gtgctgattt
     2161 ttctgggctg tctgttgggt ggtattgcta tcggtttgcc tattgcctgg gcactgttgt
     2221 tgtgcggggc ggcgttaatg ttctggctgg acatgtttga tgtccagatc atggcacaaa
     2281 cgctggtgaa cggagccgat agcttctccc tgctggcgat tccgttcttt gtgctggcgg
     2341 gtgaaatcat gaatgcgggc ggcttgtcaa aacgcattgt tgacctgccg atgaaactgg
     2401 tggggcataa accaggcggg ctgggctacg tcggcgtgct ggcagctatg attatggcga
     2461 gcctttctgg ttctgccgtt gcggataccg ccgccgttgc cgctctactg gtgccgatga
     2521 tgcgcagcgc caactatccg gtcaaccggg cggcggggct gattgcctct ggcggcatta
     2581 tcgcgccaat tattccccct tccattccgt ttattatctt cggcgtttcc agcggattat
     2641 ccatcagtaa gctgtttatg gcgggcattg cccctggcat gatgatgggc gcaacgctga
     2701 tgcttacctg gtggtggcag gccagtcgcc ttaatttacc ccgccagcaa aaagcaacga
     2761 tgcaggaaat ctggcactct tttgtctccg gtatctgggc gctgtttctt ccagtcatca
     2821 ttattggcgg gtttcgctcc ggtctgttta cccctaccga agccggggcc gttgctgctt
     2881 tctatgcgct gtttgtcgcc acagttattt accgtgaaat gaccttcgcc acactttggc
     2941 atgtactcat tggcgcggcg aaaaccacct cagtggttat gtttctggtg gcctcggcac
     3001 aagtttccgc ctggctgatt accattgctg aactgccgat gatggtttct gatttactgc
     3061 aaccgttggt cgattcaccg cgtctgctgt ttatcgtcat tatggtggct atcctgattg
     3121 tcggcatggt catggattta acgccgacgg tattaattct taccccggta ttaatgcctt
     3181 tagttaaaga ggcaggaatc gatccgattt atttcggtgt catgtttatc attaactgct
     3241 caatcggctt aattacaccg cctatcggta atgtacttaa cgttatttca ggggtggcaa
     3301 aactcaaatt cgatgatgca gtcagaggcg ttttccctta cgtcctggtt ttatattcat
     3361 tattag
//
LOCUS       DAOFQD010000244         3297 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.248, whole genome shotgun sequence.
ACCESSION   DAOFQD010000244 DAOFQD010000000
VERSION     DAOFQD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.248"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(38..799)
                     /gene="kduD"
                     /locus_tag="QBF46_004732"
     CDS             complement(38..799)
                     /gene="kduD"
                     /locus_tag="QBF46_004732"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDQ6676812.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(829..1665)
                     /gene="kduI"
                     /locus_tag="QBF46_004733"
     CDS             complement(829..1665)
                     /gene="kduI"
                     /locus_tag="QBF46_004733"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDQ6676813.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCVPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1952..3133)
                     /locus_tag="QBF46_004734"
     CDS             complement(1952..3133)
                     /locus_tag="QBF46_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDQ6676814.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          879 a          893 c          737 g          788 t
ORIGIN      
        1 tgccatgcgg cagggtcata aaagtaagaa gaatgaatta acgcgccagc caaccgccat
       61 ccacggcaat ggtataacca ttcacataat ctgaagcgct ggaggcaagg aacactaccg
      121 gccccatcag gtcactcggc agtccccaac gaccagctgg aatgcggtcg agaatttccg
      181 cgctacgttg ttcatctgcc cgcagttgtt gagtattgtt ggtcgccatg taacccggag
      241 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt cgccatcaat cgcgtcacgc
      301 ccatcacacc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
      361 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
      421 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtcct
      481 tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat
      541 caatatgacc aaactccgct accgcgcgat ccagcagtgc tggaatacca tcaatctttc
      601 gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc tgtgacctgc tcgatggttt
      661 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
      721 ccatcccctg acccagtcca gtatcacaac cagtgacgac cgcaacttta ccttcgagag
      781 aaaatgcact taaaatcata acaatacctt attcgtttat gcccacaact agcgcaaatc
      841 tttaacggcc acatggtcca tatcatcaaa gacctggttt tcaccgacca tgccccagat
      901 aaaggtataa gctttggttc cgacaccgga atggatcgac cagctcgggg agatcaccgc
      961 ctgctcgtta tgcatcacaa tatgacgcgt ttcttgcggc tgccccatca tgtggaaaac
     1021 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg
     1081 acacggcatg gtgttccaca agttacccgg agccagctcc gtcagcccca tactcaattg
     1141 gcaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag
     1201 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
     1261 cgtatgcgcg ggtacgcaat tgtaataaaa cttcgccgga gtgccggtat caatactggc
     1321 aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata
     1381 gcattggcca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
     1441 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
     1501 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
     1561 gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc
     1621 cagcgttttt gcgtgcgcac tgtggatgct ctgtcttacg tccacatcaa cctccgataa
     1681 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
     1741 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt catttttgag
     1801 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
     1861 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
     1921 agatatttat cggtaaggag gaaatgcaga gctattcgtc acgttcaatg gtcaatgcca
     1981 caccctggcc cccgccgata caaagcgttg ccagtccttt gcgggcatta cgtttcacca
     2041 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccaagtg
     2101 cgatcgcacc accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg
     2161 aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc
     2221 agcctacacg ctctaggcaa cggcgggtcg catacaccgg cgcaattccc atcaatgccg
     2281 gatctacacc aacgctggca aatgcgcgaa tgcgggccag cacgggtaaa ttcaacgctc
     2341 gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg gatgatgcat
     2401 tacccgctgt cacagaaccg agactatcaa atgaaggatt taaacgggct aaaccttctg
     2461 cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga ccgttacttt
     2521 gggtcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt
     2581 tttgttgcga gctaagtgcg taagcatcct gcaattgacg gctgatgcca tattcgcgag
     2641 ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccat aacccatcat
     2701 gcacaagact gtcaaccagc tggctattgc caagctgtgc accggtgcgg ctatcagtca
     2761 gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata tcagcctcgc
     2821 cacactgtat cgcctgagta gccagatgca gtgctttaag tccggaaccg cagacgtcat
     2881 taatcgtgat tgcagaaacg ctattaggca gaccaccttt aatagccgat tgccttgccg
     2941 gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc accgcatatg
     3001 caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt tccacggcgg
     3061 aatgacccgc taacgcaccg cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca
     3121 caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg attgttaatt
     3181 acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata tgcctgagca
     3241 gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca atagagg
//
LOCUS       DAOFQD010000245         3280 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.249, whole genome shotgun sequence.
ACCESSION   DAOFQD010000245 DAOFQD010000000
VERSION     DAOFQD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.249"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(524..1474)
                     /gene="cbl"
                     /locus_tag="QBF46_004735"
     CDS             complement(524..1474)
                     /gene="cbl"
                     /locus_tag="QBF46_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDQ6676815.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1576..2493)
                     /gene="nac"
                     /locus_tag="QBF46_004736"
     CDS             complement(1576..2493)
                     /gene="nac"
                     /locus_tag="QBF46_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDQ6676816.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGLSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            2819..2894
                     /locus_tag="QBF46_004737"
     tRNA            2819..2894
                     /locus_tag="QBF46_004737"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2852..2854,aa:Asn,seq:gtt)
     gene            3075..>3280
                     /locus_tag="QBF46_004738"
     CDS             3075..>3280
                     /locus_tag="QBF46_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDQ6676817.1"
                     /translation="MSLTDIKAKNAKPLEKEYKLTDGFGMFLRVTPKGSKYWQMAYRF
                     EGKQKLFSIGVYPAVSLSDARQRR"
BASE COUNT          912 a          786 c          695 g          887 t
ORIGIN      
        1 taaacttatc tgcggataac ggatgtgtat ttgcaataaa agatattatc aaaaatatga
       61 tggatagtgg aaagtacgat gacgccctca tttgggttga aaaattgaaa aaattgtatc
      121 ctgacacaaa taccgaactt tacatacaaa aaataacgca gaaaaaaaag caatccaaat
      181 aataattata tataattact cgcacagagt tacagtattg caagaaatat atctagcata
      241 taaagtcgcc gccagtaaag ataaagtact gtgtaatcct tcctcccata taggggtaaa
      301 aacttagcat ccagtgccct cgacttaatt tttagtattt tctctgtgaa gtgacacttc
      361 gtaggattca gttcatgttt accagaaaaa aggtggtcaa tctggtggtc ttgacaatct
      421 cgattttttg ttttagcttt ccaatcagcc agttaatggc tacggcgatc ccagtcagag
      481 gagccaaatt tctgaaaagc ccgcttttat agcgggcttt ttgctatatc tgataatcaa
      541 tttcctcttc actgttttcc atcacctgcc gcttgatatc ctcaactgac agtcctgcat
      601 tacaaagttc cagaaagcgc cagacatagt tacgctgaag ttgtcctcgc ttcagtccca
      661 accagacagt attagcatcg aaaagatgcc gcgtatccag acggattaaa ttctcttcct
      721 cttgttcgcc gctggattgt tcggcaacta atccgatccc aagcccaaga gcaacatagg
      781 ttttaatgac atcagaatcc tgcgcactta atacaatatc tgccagcaaa cctttgcggg
      841 caaatgcgtc atcaatacgt gagcgccccg taatcccctg tcggtaagtg attaacggcc
      901 acttcgctat tgattccagc gtcaatggtg taatttgcgt taagggatga tcgagtggaa
      961 caagcaaact atggtgccaa cgaaaccacg ggaaggcgac gagctgcggg tcattactca
     1021 aacgctcgct ggcgatacca atatcagctt cgccattttg caacaacgtc gcaatttcct
     1081 gtggcgtccc ctggattagc tcgagccgaa cctccgggaa aagttcgcga aaagctttaa
     1141 tgacctctgg caagctataa cgtgcctgag tatgcgtcgt tgcaatagtg agaacgccag
     1201 acgtatcgtt ggtaaacagg tctgcaagcc gacgaacatt actggcttca ttcagaatac
     1261 gttctgcaat gaccagtaat gctttgcccg gttcagtcat gcccagcagt cgcttacctc
     1321 gtcgaacaaa tatttcgatg ccaagttcat cctccagttc ccgaatatga cggctgacgc
     1381 cggactgtga ggtaaaaagc atattcgcaa cctctgtcag gttgtaatcc tgacgtgcag
     1441 cctcgcggat tatctttagt tgttggaaat tcacggtaaa ctccgggcag ttcagatttc
     1501 ccgttattgt taaagtctaa tgcccggcat aacaaataat aaaaacccgc atcttattcc
     1561 atcccgatat aacgcttagc tcaccaattg ccactgcctt ttttccatca ctggagaact
     1621 aatcactgac attaacaact ctttcactgc ctgtgcctgt ggcgataagt tcgctctggc
     1681 gggtaaattt aatgacaaag agagactcat ggaaggcgtg gtaatgcgtg acatccaccc
     1741 atttactgcg ccacataacg aacgcgcggc cgattcgggt aatactgcaa cgcccatgcc
     1801 gctggcaatc gctgcggtaa gcgtggcaat agactcaatt tcaccaatga cttttgccgt
     1861 gagtcgccgt agggaaaacg cctcatcaac acgaagtcta acagcgctgt aatcactggg
     1921 gagaaaaagg ttcatttgtg caatagcatt aacatcaacg ctttgccccg ggcaatcttg
     1981 agttcctacc aggaaaagat cttctttcag caatgcctga ctggataacc cagccacagg
     2041 ggaatgctca taaatcaccg ccatatcgag ttggtgattt atcagttttt cgttaagcac
     2101 tgcaccgcta ttttcatgaa gatagataac gatctccggg aattcagcgc gaaccgcttg
     2161 taataagggc atggtgatag atgacgcagc ggttcctggc gcaaagccaa tcgagacttg
     2221 ccccgataat gcctgaccaa cgttatgcac cgccagttgg gcctgctcac actgacgtaa
     2281 aatggcccgc gcatgggtat agagaatttt tccggcgtct gttggcgtaa cgccccgttt
     2341 tgtgcgaatc aaaagttgtt gatttaactc accttccagt gtggcaacct gctggctgag
     2401 cgctggttgt gcgatatgca atacttcagc agcctgagtc aggctaccaa tatctacaat
     2461 ttttacgaag tatttcaggc gtctgaagtt catgttgcct ccggttttta agaatcggcc
     2521 caagtgccgc cattacttac aaccagattg caagatgctt gccagtttta ttttggcgtt
     2581 gatgtacaag ctaaccaact gtcaaataag agattatgat agattcgtca ttggttcttt
     2641 ttatcagcag tcgcgttccc ctgccccata aaaggagagt atgcaccacg atggttcata
     2701 accaaaaaga ttgaaagctc accactttgt tgaaattgac agcaaacaaa caaaaaaatg
     2761 catttcaccc tttgacatca ccatgcactg ccattaatat gcgccccgtt cacacgattc
     2821 ctctgtagtt cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc
     2881 agtcagagga gccaatttaa gggaagcaga cgttcactga cgtctgcttt ctgcgtttct
     2941 atcaattggt tatccctctt cagtagttca ccctcgttca ctaaaaacca ctcgaagcca
     3001 tatcattttg atggtaaaaa tgctggtaat gctggttcga tttccctttt accaacaaat
     3061 gagggggtgt tttcatgtca ctgactgata ttaaagcaaa aaatgcaaaa ccccttgaga
     3121 aggaatacaa gctgactgat ggctttggta tgttccttcg cgttaccccg aagggttcca
     3181 aatactggca aatggcttac cgcttcgaag ggaaacaaaa actcttctct attggtgttt
     3241 accctgctgt ttctctttct gacgcaaggc aacgccgtga
//
LOCUS       DAOFQD010000246         3280 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.250, whole genome shotgun sequence.
ACCESSION   DAOFQD010000246 DAOFQD010000000
VERSION     DAOFQD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.250"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1023)
                     /gene="rspA"
                     /locus_tag="QBF46_004739"
     CDS             complement(<1..1023)
                     /gene="rspA"
                     /locus_tag="QBF46_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDQ6676818.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQE"
     gene            complement(1229..1555)
                     /locus_tag="QBF46_004740"
     CDS             complement(1229..1555)
                     /locus_tag="QBF46_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDQ6676819.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1690..2031
                     /locus_tag="QBF46_004741"
     CDS             1690..2031
                     /locus_tag="QBF46_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDQ6676820.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2066..2626
                     /gene="speG"
                     /locus_tag="QBF46_004742"
     CDS             2066..2626
                     /gene="speG"
                     /locus_tag="QBF46_004742"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDQ6676821.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(2629..>3280)
                     /locus_tag="QBF46_004743"
     CDS             complement(2629..>3280)
                     /locus_tag="QBF46_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDQ6676822.1"
                     /translation="EVTLSFTPEMASFSNEFDFDPLRGPVKDFTQTLMDEQGEVTKRV
                     SGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAETLEKRVRLQGKCQLAELPSAG
                     VSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKSNDKTLSVSATPSTDPIKKLD
                     YTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEGVKPAVERVYTIKNTIDYY"
BASE COUNT          906 a          818 c          733 g          823 t
ORIGIN      
        1 ttcctggaca ccgaaattgg ggacccacag atcaaagtgc agcgccgcag ccatgcagac
       61 tggtgacaaa tcggaaggac cgtgtgagcc agtacgtacc tgatacagcg aagcaaaatc
      121 ggcaatccgg cgcataccgg taattccgcc tgcatgggtc agcgtggtgc ggatataatc
      181 gatgagttgc tcttcaatca gttgtttgca gtcccagatg ctgttgaaga cttcacccac
      241 tgcgatgggt gtgacggtat gttggcgaat gagacggaag cattcctggt tttccgcagg
      301 cgtcgggtct tccatccaga acatgcgata atcttcaatg cttttaccaa agcgcgccgc
      361 ttcaataggc gttaagcgat ggtgcatgtc atgcagcaaa tgttcattaa aaccaaactt
      421 gttacgtacc gcgtcaaaca atttcggcat gaaatcgagg tatttctccg tcgaccacag
      481 ctgctcttcc ggccactgtc ctttggttgc gggttcataa gccagacctt tacctttcga
      541 catgccgtag gtggttttca taccagggat tccgcactgc acgcggatgg ctttgaatcc
      601 aagctcttga tgacgggcat aatcatccag agcttcatca atactgtgac cggtggtatg
      661 gcaataaacc atcacccctt cacgagacgc gccgccgagt aactggtaaa gcggcatgtt
      721 ggcagctttg gctttaatat cccacagcgc catatcaacc gctgaaatgg ccgacatcgt
      781 aaccggaccg cgacgccagt aagcaccttt atagaaaaac tgccagatat cttcgatacg
      841 gtgcgcatcg cgaccaataa gctgcggaca aaggtgatcc tgcaaataag aggccacgga
      901 aagctcacgt ccattgaggg tggcatcccc aaggcccgta ataccgtcct cagtggtgat
      961 ttttaatgtg acgaaattac gccccggaca ggtaacaaaa acttcagcct ttacgatctt
     1021 catgttcgat tccttgcatc gcttgtcgtg atgcatgaaa tctacgcaac tgagctacta
     1081 ccatacaagt ataaagatcg aaaaaagccg gagtgatcac aaaaaaaggc gtatatttgc
     1141 gctgtgaatg gttgacaaaa gatgaaatag aatacctttt gtcagctgac acttcctcgt
     1201 atcttattga taaaatggat ttatgttcct acgtgcgccc ccagcccgca acaatgatca
     1261 acattccgca aagcgcaatc aacgcacccg tccagtcata aagacttagt ttcacgccat
     1321 ccacaacgcg cagccacatc aatgccgtgc agacataaac gccaccataa gccgcgtaaa
     1381 cacgcccact cgccgcagga tgcaacgtta acaaccagac aaacagcgcc agtgaaatcc
     1441 ccgccggaag caacagccag atactggcgt ttcgttttaa ccacaaccag ggcagaaagc
     1501 atccaataat ttcacacagc gcagtagcaa aaaatagtaa cgttgtttta atcatctttg
     1561 tcacttattg acatcatgta tagttatagg gcgacataat atcatcaata taaacatcct
     1621 cctggtacgt tttgcgttcg cagtggatgg tgtagaatca cctttaatca ttcatacagg
     1681 gaatgaatta tgaaaatcac tctcagcaaa cgaatcggcc tgctcgcttt tctgctgcct
     1741 tgcgcactgg cattgagcac aactgttcat gccgaaacta acaaactggt gattgagtct
     1801 ggcgacagtg cacaaagccg ccagcacgcc gctatggaaa aagagcaatg gaatgacacg
     1861 cgcaatctgc gccagaaagt gaataaacgc actgaaaaag agtgggataa agccgacgcc
     1921 gcttttgata accgcgataa atgtgagcaa agcgccaaca tcaatgccta ctgggagccc
     1981 aatactttgc gctgcctgga ccgtcgaact ggccgcgtta ttacccccta acctgttatt
     2041 gatttaagga atgtaaggac acgttatgcc aagcgcccac agtgttaagc tacgcccgct
     2101 ggagcgtgaa gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta
     2161 ctggtttgag gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcatat
     2221 tcacgatcag agcgaacggc gctttgtggt ggaatgtgac ggcgaaaaag ccggtctggt
     2281 ggagctggtg gaaattaacc atgttcatcg ccgcgcagaa tttcagataa ttatctcccc
     2341 ggagtatcag gggaaaggtc tggcaacccg tgccgccaaa ttagcaatgg actatggctt
     2401 taccgttctc aatctctata agctgtatct gatcgttgat aaagagaatg aaaaagcgat
     2461 tcacatttac cgcaagcttg gcttttcggt tgaaggtgaa ttgatgcacg agttctttat
     2521 taatggtcaa tatcgtaatg ccattcgcat gtgtatattc cagcatcagt atctggcaga
     2581 gcacaaaaca ccgggtcaga ctctcctgaa gccgaccgca caatagcatt aataataatc
     2641 gatcgtattt ttgatggtgt aaacccgttc gacggcgggt tttactcctt catcaacaat
     2701 gattagctga cagtccaccg gattagcgtg actgtcatat tcacagctct gttttacatt
     2761 accaacccgt tgattattca gtaaagtaac cgctgtgtaa tctaattttt tgatcggatc
     2821 cgttgatggc gtggcgctga cagataatgt tttgtcgtta cttttcgtgg ttttacccag
     2881 cggataaccc tgatcatcat agcgatattc catctgcatt tgtttgctgc tggctttaat
     2941 cacgaagcca ttatcatcgg tttcccaact caccccggca gaaggtaatt ctgctagctg
     3001 gcattttccc tgtaaacgta ctctcttctc cagcgtctcg gcatcacggt aataattggc
     3061 gtccagtacc agagcgacca cggtattatt ttccagatcc agtaattcga gtgaatcaaa
     3121 acagccttct tccgacaaag tcccagaaac acgtttcgtc acttcacctt gctcatccat
     3181 taatgtctga gtgaaatctt ttaccggacc acgcagcgga tcaaaatcga attcattaga
     3241 gaaactggcc atctcagggg taaatgaaag tgttacctct
//
LOCUS       DAOFQD010000247         3196 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.251, whole genome shotgun sequence.
ACCESSION   DAOFQD010000247 DAOFQD010000000
VERSION     DAOFQD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.251"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..921
                     /locus_tag="QBF46_004744"
     CDS             <1..921
                     /locus_tag="QBF46_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDQ6676823.1"
                     /translation="KWGFYLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMG
                     LIVRAGFKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPE
                     PGKDTFQYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKAN
                     MEEAPEQVIALLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNG
                     TIEEELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYR
                     YDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(1038..2381)
                     /gene="gdhA"
                     /locus_tag="QBF46_004745"
     CDS             complement(1038..2381)
                     /gene="gdhA"
                     /locus_tag="QBF46_004745"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDQ6676824.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2617..2889
                     /locus_tag="QBF46_004746"
     CDS             2617..2889
                     /locus_tag="QBF46_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDQ6676825.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2855..>3196)
                     /gene="nudG"
                     /locus_tag="QBF46_004747"
     CDS             complement(2855..>3196)
                     /gene="nudG"
                     /locus_tag="QBF46_004747"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDQ6676826.1"
                     /translation="PAQSDQAGLWEFAGGKVELDESQQQALVRELNEELGIEATVGEY
                     VASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIPLLEA
                     FMALRAARAAD"
BASE COUNT          816 a          792 c          776 g          812 t
ORIGIN      
        1 aagtgggggt tttatttaac atgtgttgtc gctgtaatgt ttgtgttcgc agcgataaca
       61 tccaacggtt tgaatgaaag aggcctgatt accgcgggat gctcttttct ttatctatta
      121 attatgatgg ggcttattgt tcgggccggt tttaaagcaa aaaaagaaca actgcattat
      181 tatcaggcta aaggtattga gccgctcagt atcgagaagt tacaggcgct acaattgatc
      241 gcaccttatc gattctatca taagcaatgg tctgaaacgc tggagttctg gccgcgaaag
      301 cctgaacctg gcaaagatac cttccaatat catgtgcttc cttttgactc gatcgatatc
      361 ataagtaaaa gacgcgagtc tttagaggat caatggggta tcgaagatag cgaaagttat
      421 tgtgccttaa tggagcattt tctttctggc gaccatggag ccaatacctt taaagcaaac
      481 atggaggaag ccccagagca ggttatcgcc ttgttgaata aatttgctgt ttttccctca
      541 gactatatct ctgattgcgc taataatcgc tccggtaaat cctcggcgaa gctaatatgg
      601 gcggcggaat tatcatggat gatctcgata tcaagcacag cttttcaaaa cgggacaatt
      661 gaagaagaac tggcctggca ttatataatg cttgcttctc gaaaggcgca cgagttgttc
      721 gaaagcgaag aagattatca aaaaaatagt ctaatgggat ttctttactg gcatatctgc
      781 tgctatcgca gaaagttaac ggatgctgaa cttgaagcgt gttatcgtta cgacaagcag
      841 ttttgggagc actacagtaa gaaatgccgt tggcccataa gaaatgttcc ctggggagca
      901 tcatccgtta aatactcata aacgcctgaa attttgccgg gggcgctgcg cttacccggc
      961 ctacattaga gcgtaactgc aatgaattgt gcccatttgt aggcctgata agcgtagcgc
     1021 catcaggcat ttacaactta aatcacaccc tgcgccagca tcgcatcggc aaccttcaca
     1081 aaaccggcaa tgttcgcgcc ctgcacgtag ttggtttgct caccttcacc accatgctca
     1141 acacaggcat ggtggatatc cagcatgatg tgatgcaaac gtgcgtcaac tttctcggct
     1201 ttccagccca ggcgcgcagc gttttgtgcc atttccaggc ctgacgttgc tacaccgcca
     1261 gcattagccg ctttacccgg tgcaaatagt acgcctgcct gctggaacag ttcagtcgct
     1321 tcgatggtgg tcggcatatt tgccccttcg gcgacggctt taacgccatt ggcgataagc
     1381 tgatgcgcgg cgtcaacatc cagttcgttc tgggtggcgc aaggcagggc gatatcaacc
     1441 ggtacagacc acggctgttg gccttcgaga tagaccagac caaattcttt ggcgtaatct
     1501 gccactcggc catcgcggct ggctttgatt tcgataagac gtgccagttt ctctttcgtg
     1561 aatccgcttt catcaactac agtgccactg gagtctgacg cagtgatcac acgagcacca
     1621 aattccatcg ctttttcgat agcgtactgg gcgacgttgc cggagccaga aacggaaacg
     1681 cgcattcctt caaaacccat accgtggcgt tttagcattg cttctgtgaa ataaaccaga
     1741 ccgtagccgg tagcttccgg gcgaataaga ctaccgccaa atgaaaggcc cttaccggtg
     1801 aagacgcagg cggtattgtt ggagagcttt ttcatcatcc ccgccataaa gccgacttca
     1861 cgaccaccaa ccccgatatc acctgccgga acgtcggtat ccgcgcccag gtggcgatac
     1921 agttcagtca tcagcgcctg gcaaaaacgc atcacttcac cttcgctttt tcctttcgga
     1981 tcgaaatcgc tgccgccttt accaccgccc atcggcagag tagtcagggc atttttgaag
     2041 gtttgttcaa agccgaggaa tttgagaatg gaaaggttaa ctgacggatg gaagcgcata
     2101 ccgcctttgt acgggccgat ggctgagctg aactgcacac gccatgcacg gttgacctgt
     2161 acctggttgc gatcatcaac ccataccacg cgaaactgga tcacgcgctc cggttcaacc
     2221 agacgctcca gtaatgacat ctggcgatat tttggatttt gttcaagaaa aggccagagt
     2281 gtggtcatta cttcacgaac ggcttgcgcg aactcggttt gattcgggtc gcgcttttgg
     2341 acatggttga ggaatgactc cagagaatat gtctgatcca tagatataaa acccttatat
     2401 attaatacga ttgtgcttat gttgtttatg tcatgtgctt ttgcagtttt cgactatacc
     2461 atcaagaatg tgacggtaag caagaaaaaa atgacccagg aaagcaaatt aataacgaga
     2521 gtaatctcat aaccaaatgc gataactcag acgctaaagt tttgccaccc ggctaccgtt
     2581 atcagttgca gagacaataa cgttaaggag ttccctgtga gtcgagcatt gttcgccgta
     2641 gtattagcat ttccattaat tgcactggct aacccgcatt atcgtccgga cgtggaggta
     2701 aacgtaccgc cagaagtgtt cagttcaggc gggcagtcag cgcagccctg tactcagtgc
     2761 tgcgtttatc aggatcaaaa ttattctgaa ggggcggtaa ttaaagccga aggaatactg
     2821 ctgcaatgcc agcgggatga caaaaccctt agcactaatc cgctgctctg gcggcgcgta
     2881 aagccataaa cgcctctaat agtggaatgt cagcaggggc cagcggatat tgcagggcct
     2941 cttcaggtga gcaccagacc agcgcctgat gttcatgtgc ctgtaacgtc ccgtggaagt
     3001 cgggtacgtg ccaggcatga agatggataa tccggcccga aacttctcgc tgatggctgg
     3061 caacatactc acccacagtt gcttcgatgc ccaattcttc gtttaactca cgcaccagtg
     3121 cctgctgctg gctttcatcc agctcgactt taccaccggc aaactcccat aatcccgcct
     3181 gatcgctctg ggcggg
//
LOCUS       DAOFQD010000248         3110 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.252, whole genome shotgun sequence.
ACCESSION   DAOFQD010000248 DAOFQD010000000
VERSION     DAOFQD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.252"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>3110
                     /locus_tag="QBF46_004748"
     CDS             <1..>3110
                     /locus_tag="QBF46_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit F"
                     /protein_id="HDQ6676827.1"
                     /translation="LAALWLGRLCNRMDYAAGFIFMRRLGSAALTATGPVLNVLPLGI
                     HIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRDSGRAAGDEPLFGPVLNIKVFDY
                     QLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSIEILANKQRYDEPTLIQHAERLK
                     MLIAQFAADPALLCGDVDIMLPGEYAQLAQLNATQVEIPETTLSALVAEQAAKTPDAP
                     ALADARYLFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGA
                     AWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRFSDVPNLTSLCYNAPLTPQGSAP
                     LQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRLLWMQNHYPLTGEDVVAQKTPCS
                     FDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFFAEYGVTTTHFVPSMLAAFVASL
                     TPQTARQSCATLKQVFCSGEALPADLCREWQQLTGAPLHNLYGPTEAAVDVSWYPAFG
                     EELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGVAGDLYLTGIQLAQGYLGRPDLT
                     ASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRSDDQLKIRGQRIELGEIDRVMQA
                     LPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSGLPLDTSALQAQLRETLPPHMVP
                     VVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAPKAGSETIIAAAFSSLLGCDVQD
                     ADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVMVASTVAKLATIIDAEEDSTRRM
                     GFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYLDPQWSIIGIQSPRPNGPMQTAA
                     NLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQGIAARLRARGEQVAFLGLLDTWP
                     PETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGNYADAVRLLTT
                     AHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDIISPGTFEKIG
                     PIIRATL"
BASE COUNT          642 a          835 c          932 g          701 t
ORIGIN      
        1 ctggcggctt tgtggctggg gcgattgtgc aaccgcatgg actacgctgc cggatttatc
       61 tttatgcgtc gactgggctc ggcggcgttg acggctaccg gacccgtgct caacgttttg
      121 ccgttgggta ttcatattgc ggcgcaagaa acgctgccgg aactggcaac ccgactggcg
      181 gctcaactga aaaaaatgcg tcgtcatcaa cgttacgatg ccgaacaaat tgtccgtgac
      241 agcgggcgag cggcaggtga tgaaccgctg tttggtccgg tactcaatat caaggtattt
      301 gattaccaac tggatattcc tggtgttcag gcgcaaaccc ataccctggc aaccggtccg
      361 gttaatgacc ttgaactggc cctgttcccg gatgaacacg gtgatttgag tattgagatc
      421 ctcgccaata aacagcgtta cgatgagcca acgttaatcc agcatgctga acgcctgaaa
      481 atgctgattg cccagttcgc cgcggatccg gcgctgttgt gcggcgatgt cgatattatg
      541 ctgccaggtg agtatgcgca gctggcgcag ctcaacgcca ctcaggttga gattccagaa
      601 accacgctta gcgcgctggt ggcagaacaa gcggcaaaaa caccggatgc tccggcgctg
      661 gcagatgcgc gttacctgtt cagctatcgg gaaatgcgcg agcaggtggt ggcgctggcg
      721 aatctgctgc gtgagcgcgg cgttaaacca ggggacagcg tggcggtggc actaccgcgc
      781 tcggtctttt tgaccctggc actccatgcg atagttgaag ctggagcggc ctggctaccg
      841 ctggataccg gctatccgga cgatcgcctg aaaatgatgc tggaagatgc gcgtccgtcg
      901 ctgttaatta ccaccgacga tcaactgccg cgctttagcg atgttcccaa tttaacaagc
      961 ctttgctata acgccccgct tacaccgcag ggcagtgcgc cgctgcaact ttcacaaccg
     1021 catcacacgg cttatatcat ctttacctct ggctccaccg gcaggccgaa aggggtaatg
     1081 gtcgggcaga cggctatcgt caaccgcctg ctttggatgc aaaatcatta tccgcttaca
     1141 ggcgaagatg tcgttgccca aaaaacgccg tgcagttttg atgtctcggt gtgggagttt
     1201 ttctggccgt ttatcgcagg ggcaaaactg gtgatggctg aaccggaagc gcaccgcgac
     1261 ccgctcgcta tgcagcaatt ctttgccgaa tatggcgtaa cgaccacgca ctttgtgccg
     1321 tcgatgctgg cggcatttgt tgcctcgctg acgccgcaaa ccgctcgcca gagttgcgcg
     1381 acgttgaaac aggttttctg tagtggtgag gccttaccgg ctgatttatg ccgcgaatgg
     1441 caacagttaa ctggcgcgcc gttgcataat ctatatggcc cgacggaagc ggcggtagat
     1501 gtcagctggt atccggcttt tggcgaggaa ctggcacagg tgcgcggcag cagtgtgccg
     1561 attggttatc cggtatggaa tacgggtctg cgtattcttg atgcgatgat gcatccggtg
     1621 ccgccgggtg tggcgggtga tctctatctc actggcattc aactggcgca gggctatctc
     1681 ggacgccccg atctgaccgc cagccgcttt attgccgatc cttttgcccc aggtgaacgg
     1741 atgtaccgta ccggagacgt tgcccgctgg ctggataacg gcgcggtgga gtacctcggg
     1801 cgcagtgatg atcagctaaa aattcgcggg cagcgtatcg aactgggcga aatcgatcgc
     1861 gtgatgcagg cgctgccgga tgtcgaacaa gccgttaccc acgcctgtgt gattaaccag
     1921 gcggctgcca ccggtggtga tgcgcgtcaa ttggtgggct atctggtgtc gcaatcgggc
     1981 ctgccgttgg ataccagcgc attgcaggcg cagcttcgtg aaacattgcc accacatatg
     2041 gtaccggtgg ttctgctgca acttccacag ttaccactta gcgccaacgg caagctggat
     2101 cgcaaagcct taccgttgcc tgaactgaag gcacaagcgc cagggcgtgc gccgaaagcg
     2161 ggcagtgaaa cgattatcgc cgcggcattc tcgtcgttgc tggggtgtga cgtgcaggat
     2221 gccgatgctg atttcttcgc gcttggcggt cattcgctac tggcaatgaa actggcagcg
     2281 cagttaagtc ggcaggttgc ccgccaggtg acgccggggc aagtgatggt cgcgtcaact
     2341 gtcgccaaac tggcaacgat tattgatgct gaagaagaca gcacccggcg tatgggattc
     2401 gaaaccattc tgccgttgcg tgaaggtaat ggcccgacgc tgttttgttt ccatcctgcg
     2461 tccggttttg cctggcagtt cagcgtgctc tcgcgttatc tcgatccaca atggtcgatt
     2521 atcggcattc agtcaccgcg ccccaatggc cccatgcaga cggcggcaaa cctggatgaa
     2581 gtctgcgaag cgcatctggc aacgttactt gaacaacaac cgcatggccc ttattacctg
     2641 ctggggtatt cccttggcgg tacgctggcg cagggtattg cggcgcgact gcgtgcccgt
     2701 ggcgaacagg tggcatttct tggcttgctg gatacctggc cgccagaaac gcaaaactgg
     2761 caggaaaaag aagctaatgg tctggacccg gaagtgctgg cggagattaa ccgcgaacgc
     2821 gaggccttcc tggcagcaca gcagggaagt acttcaacgg agttgtttac caccattgaa
     2881 ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc gtttgacggt
     2941 aaagcgacgc tgtttgttgc tgaacgcaca cttcaggaag gtatgagtcc cgaacgcgcc
     3001 tggtcgccgt ggatagcgga gctggatatc tatcgtcagg attgtgcgca tgtggatatt
     3061 atctctccag ggacgtttga aaaaattggg ccgattattc gcgcaacgct
//
LOCUS       DAOFQD010000249         3051 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.253, whole genome shotgun sequence.
ACCESSION   DAOFQD010000249 DAOFQD010000000
VERSION     DAOFQD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.253"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..191
                     /gene="lacI"
                     /locus_tag="QBF46_004749"
     CDS             <1..191
                     /gene="lacI"
                     /locus_tag="QBF46_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="transcriptional repressor of lac operon; forms a
                     homotetramer as a dimer of dimers; binds specific sites in
                     lac operon resulting in DNA looping between the operators;
                     binds allolactose as inducer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lac repressor"
                     /protein_id="HDQ6676828.1"
                     /translation="TSVDRLLKLSQGQAVKSNQLLPVSLVKRKTTLAPNTQTTSPRTL
                     ADSLMQLARQVSRLESGQ"
     gene            314..>3051
                     /gene="lacZ"
                     /locus_tag="QBF46_004750"
     CDS             314..>3051
                     /gene="lacZ"
                     /locus_tag="QBF46_004750"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDQ6676829.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQESRSLNGEWRFAWFPAPEAVPESWLERDLPDADTVIVPSNWQMHGYDAPIYT
                     NVTYPIAVNPPYVPTENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLHAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDLHVATHFNDDFSRAVLEADVQIYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADHVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFSLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATTWSAAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASNTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTA"
BASE COUNT          701 a          838 c          858 g          654 t
ORIGIN      
        1 aaaccagcgt ggaccgcttg ctgaaactct ctcagggcca ggcggtgaag agcaatcagc
       61 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccacct
      121 ctccccgcac gttggcagat tccttaatgc agctggcacg acaagtttcc cgacttgaaa
      181 gcgggcagtg agcgcaacgc aattaatgtg agtcagctca ctcattaggc accccaggct
      241 ttacactcta tgtgtccggc tcgtatgtta tgcgaaaatg tgagcggata acaatttcac
      301 acaggataca actatgacaa tgattacgga ttcactggcc gtcgtattac aacgtcgtga
      361 ctgggaaaac cctggcgtta cccaacttaa tcgccttgcg gcacatcccc ctttcgccag
      421 ctggcgtaat agcgaagagg ctcgcaccga tcgcccttcc caagagtcgc gcagcctgaa
      481 tggtgaatgg cgctttgcct ggtttccggc accagaagcg gtaccagaaa gctggctgga
      541 gcgcgatctt cctgacgccg atactgtcat agtcccctca aactggcaga tgcacggtta
      601 cgatgcgcct atctacacca acgtgaccta tcccattgcg gtcaatccgc cgtatgttcc
      661 cacggagaat ccgacgggtt gttactcgct cacatttaat attgatgaaa gctggctaca
      721 ggaaggccag acgcgaatta tttttgatgg tgttaactcg gcgtttcatt tgtggtgcaa
      781 cgggcgctgg gtcggttacg gacaggacag tcgtttgccg tctgaatttg acctgagcgc
      841 atttttacac gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag
      901 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct
      961 gcataaaccg accacgcaaa tcagcgattt acatgtagcc actcacttta atgatgattt
     1021 cagccgcgct gtactggagg cagacgttca gatatacggc gagctgcgcg atgagctgcg
     1081 ggtgacggtt tctttgtggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg
     1141 cggtgaaatt atcgatgagc gtggtggtta tgccgatcac gtcaccctac gtctgaacgt
     1201 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcag tggttgaact
     1261 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt
     1321 acggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gcggcgttaa
     1381 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga
     1441 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca
     1501 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaacat
     1561 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctacccgc
     1621 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat
     1681 ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcactct atcgctggat
     1741 caaatctgtc gatccttccc gcccggtaca gtatgaaggc ggcggagccg acaccttcgc
     1801 aaccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt
     1861 accgaaatgg tccatcaaaa aatggctttc gttgcctgga gagacgcgcc cgctgatcct
     1921 ttgcgaatac gcccacgcga tgggtaacag tcttggcggc ttcgctaaat actggcaggc
     1981 gtttcgtcag tacccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct
     2041 gattaaatat gatgaaaatg gcaatccgtg gtcggcttac ggcggtgatt ttggcgatac
     2101 gccgaatgat cgccagttct gcatgaacgg tctggtcttt gccgaccgca cgccgcatcc
     2161 ggcgctgacg gaagcaaaat accagcagca gtttttccag ttcagtttat ccgggcaaac
     2221 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat
     2281 ggtggcgctg gatggcaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca
     2341 aggtaaacag ttgattgaac tgcctggact accgcagccg gagagcgccg gacaactctg
     2401 gctaacggtt cacgtagtgc aaccgaacgc gaccacatgg tcagcagccg gacacatcag
     2461 cgcctggcag cagtggcgtc tggcggaaaa cctcagcgtg acactcccct ccgcgcccca
     2521 cgccatcccg caactgacca ccagcgaaac ggatttttgc atcgagctgg ataataagcg
     2581 ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca
     2641 actgctgacg ccgctgcgcg atcagttcac ccgcgcaccg ctggataacg acattggcgt
     2701 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca
     2761 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acgcttgccg acgcggtgtt
     2821 gattaccacg gtccacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac
     2881 ctaccggatt gatggaagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcaa
     2941 tacgccacat ccggcgcgga ttggcctgac ctgccagctg gcgcaggtag cagagcgggt
     3001 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg c
//
LOCUS       DAOFQD010000250         3041 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.254, whole genome shotgun sequence.
ACCESSION   DAOFQD010000250 DAOFQD010000000
VERSION     DAOFQD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.254"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..191
                     /gene="lacI"
                     /locus_tag="QBF46_004751"
     CDS             <1..191
                     /gene="lacI"
                     /locus_tag="QBF46_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="transcriptional repressor of lac operon; forms a
                     homotetramer as a dimer of dimers; binds specific sites in
                     lac operon resulting in DNA looping between the operators;
                     binds allolactose as inducer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lac repressor"
                     /protein_id="HDQ6676830.1"
                     /translation="TSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRAL
                     ADSLMQLARQVSRLESGQ"
     gene            314..>3041
                     /gene="lacZ"
                     /locus_tag="QBF46_004752"
     CDS             314..>3041
                     /gene="lacZ"
                     /locus_tag="QBF46_004752"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDQ6676831.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TVRPSPQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVIVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNINESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPD"
BASE COUNT          684 a          839 c          869 g          649 t
ORIGIN      
        1 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
       61 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
      121 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaagtttcc cgactggaaa
      181 gcgggcagtg agcgcaacgc aattaatgtg agtcagctca ctcattaggc accccaggct
      241 ttacacttta tgcgtccggc tcgtatgttg tgtgaaattg tgagcggata acaatttcac
      301 acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgtattac aacgtcgtga
      361 ctgggaaaac cctggcgtta cccaacttaa tcgccttgcg gcacatcccc ctttcgccag
      421 ctggcgcaat agcgaagaag cccgcactgt tcgcccctcc ccgcagttgc gcagcctgaa
      481 tggtgagtgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga
      541 gtgcgatctt cctgacgccg atactgtcat cgtcccctca aactggcaga tgcacggtta
      601 cgacgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc
      661 cacggagaat ccgacgggtt gttactcgct cacatttaat attaatgaaa gctggctaca
      721 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa
      781 cgggcgctgg gtcggttacg gccaggacag ccgtttgccg tctgaatttg acctgagcgc
      841 atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggtag
      901 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct
      961 gcataaaccg accacacaaa tcagcgattt ccatgttgcc actcacttta atgatgattt
     1021 cagccgcgcg gtactggaag cagaagttca gatgtatggc gagctgcgcg atgagctgcg
     1081 ggtgacggtt tctttgtggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg
     1141 cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt
     1201 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact
     1261 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt
     1321 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gcggcgttaa
     1381 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga
     1441 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca
     1501 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtagatg aagccaacat
     1561 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc
     1621 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat
     1681 ctggtcgctg ggaaatgagt caggccacgg cgctaatcac gacgcactct atcgctggat
     1741 caaatctgtc gatccatccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc
     1801 caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt
     1861 gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct
     1921 ttgcgaatac gcccacgcga tgggtaacag tcttggcggc ttcgctaaat actggcaggc
     1981 gtttcgtcag tacccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct
     2041 gattaaatat gatgaaaatg gcaatccgtg gtcggcttac ggcggtgatt ttggcgatac
     2101 gccgaacgat cgccagttct gcatgaacgg tctggtcttt gccgaccgca cgccgcatcc
     2161 agcgctgacg gaagcaaaac accagcaaca gtttttccag ttccgtttat ccgggcaaac
     2221 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat
     2281 ggtggcgctg gatggcaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca
     2341 aggtaaacag ttgattgaac tgcctggact accgcagccg gagagcgccg gacaactctg
     2401 gcttacggta cacgtagtgc aaccgaacgc gaccgcatgg tcagaagccg gacacatcag
     2461 cgcctggcag cagtggcgtc tggcggaaaa cctcagcgtg acactccccg ccgcgtccca
     2521 cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg
     2581 ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca
     2641 actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt
     2701 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca
     2761 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct
     2821 gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac
     2881 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga
     2941 tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt
     3001 aaactggctc ggattagggc cgcaagaaaa ctatcccgac c
//
LOCUS       DAOFQD010000251         3006 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.255, whole genome shotgun sequence.
ACCESSION   DAOFQD010000251 DAOFQD010000000
VERSION     DAOFQD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.255"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            11..2308
                     /locus_tag="QBF46_004753"
                     /pseudo
     CDS             11..2308
                     /locus_tag="QBF46_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4092 domain-containing protein"
     gene            complement(2271..2555)
                     /locus_tag="QBF46_004754"
     CDS             complement(2271..2555)
                     /locus_tag="QBF46_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676832.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTLADRYLLPTC"
     gene            complement(2645..2971)
                     /gene="yafW"
                     /locus_tag="QBF46_004755"
     CDS             complement(2645..2971)
                     /gene="yafW"
                     /locus_tag="QBF46_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system antitoxin YafW"
                     /protein_id="HDQ6676833.1"
                     /translation="MSNPTRGLQREITLRLGARLVQEGNRLHYLADRTSITGKFSDIE
                     CRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYHNDLTCEADTFGSCGYVYLAVYP
                     TPETKK"
BASE COUNT          758 a          711 c          833 g          704 t
ORIGIN      
        1 tgcgttatta atgaataaga aatttaaata taagaaatcg cttttagcgg ctattttgag
       61 cgcaaccctg ttagccggtt gtgatggcgg tggttccaga tcttcctccg atacgccgcc
      121 tgtagattct ggaacagggt ctttgccgga agtgaaacct gatccaacac caaacccgga
      181 gccgacgcct gagccaacgc cggagcctga acctacgccg gaaccgacac ctgatccaga
      241 tccagagcca gagccagagc cagagcctgt tcctacgaaa acgggttatc tgaccctggg
      301 cggaagccag cgggtaactg gtgctaaatg taatggtgaa aacagcgatg gctttacatt
      361 tacacctggc gaggacgtta cttgcgtggc gggtaacacg acaattgcca ccttcaacac
      421 tcagtcagaa gctgcgcgta gcctgcgtgc ggttgaaaaa gtgtcgttta gtcttgagga
      481 cgcgcaagaa ctggcggctt ccgacaacaa gaaaagcaat gcggtttcgc tggtaacgtc
      541 catgaacagt tgtccggcgg atacagaaca ggtgtgcctg gagttctcct cagtgatcga
      601 gagtaaacgc ttcgactcgc tgtataagca aatcgatctg gcaccggaag agttcaaaaa
      661 gctggtcaat gaagaggtgg aaaacaatgc cgcgaccgat aaagcgccat ccactcatac
      721 ttcaccggtc gtgcccgcca ccactccggg aacaaaaccg gatctaaacg cttccttcgt
      781 gtcggctaac gcggaacagt tttatcagta tcaacccact gaaatcattc tctctgaagg
      841 tcgactggtc gatagtcagg gggatggtgt tgttggtgtc aactattaca ccaattccgg
      901 ccgtggtgta accggagaaa acggggaatt ttcctttagt tggggggaaa ccatctcctt
      961 tggcatcgac acttttgagc ttggttctgt gcgtggtaac aagtcgacta ttgcattgac
     1021 tgaactgggt gatgaagttc gcggggcaaa tatcgatcag ttgattcacc gctattcgaa
     1081 ggctggacaa aatcacacgc gtgtagttcc ggatgaagta cgcaaggttt ttgctgaata
     1141 tcccaacgtg attaacgaga ttatcaatct ctcgttatcc aatggtgcga cgctggggga
     1201 aggtgagcaa gtcgttaatc tgcctaacga atttatcgag cagtttaaga cgggtcaggc
     1261 caaagagatc gataccgcga tttgtgcgaa aaccgacggt tgtaacgagg ctcgctggtt
     1321 ctcgctgacg acgcgcaatg ttaacgaagg tcagattcag ggcgttatca acaagctgtg
     1381 gggtgtggat aaagattaca aatcggtgac gaaattccac gtcttccatg actctaccaa
     1441 cttctatggc agcaccggta atgcgcgcgg tcaggcggtg gtgaatatct ccaacgcggc
     1501 cttcccgatt ctgatggcgc gtaatgataa aaactactgg ctggccttcg gcgagaaacg
     1561 ggcctgggat aaaaatgagc tggcgtacat tactgaagcg ccttccattg tgcgaccaga
     1621 gaacgtgaca cgcgaaaccg ccaccttcaa cctgccgttt atttcgctgg ggcaagtggg
     1681 cgatggcaag ctgatggtta tcggtaaccc acactacaac agcatcctgc gttgcccgaa
     1741 cggttacagc tggaacgggg gcgttaataa agatgggcag tgtacgctca acagcgaccc
     1801 ggatgacatg aagaacttca tagagaacgt gctgcgctat ctgtcaaatg atcgctggtt
     1861 gccggatgca aaatccaata tgaccgtggg tactaacctg gacacggtgt atttcaaaaa
     1921 acacgggcag gttacaggaa atagtgctgc gttcggcttt catccggatt ttgcgggtat
     1981 ctctgttgag catttaagta gctatggcga tctcgacccg caggaaatgc cgctgctgat
     2041 cctcaacggc tttgagtatg tgactcaggt tggtaacgat ccttatgcaa tcccgctgcg
     2101 tgcagatacc agcaaaccga agctgaccca gcaggatgtg accgatttga tcgcctatat
     2161 gaacaaaggt ggatcggtgc tgatcatgga aaacgtgatg agcaatctta aggaagagag
     2221 cgcatctggc tttgtacgtc tgcttgatgc cgcaggtttg tcgatggcgc ttaacaagtc
     2281 ggtagtaaat aacgatccgc aagggtacag gcgctgaatc ccggctggtc ggtacgtacc
     2341 agtgcgtatt tttcaacgag aaagttcacc gcatcacaca gtgaaatgcc agcttcaata
     2401 tgctgctcaa tcacacgttc atcggcgaac ggtgtgtcat tcagcgtaag gccgtaatgc
     2461 tggtccagca accgggacag cagtgtctgc cagatggtga cgggagacgg gcagcgcgat
     2521 gcctcccgta cgtgtgtgtc gggtaatgtt ttcatactga agattttcct gatatgcaga
     2581 gatgaaaaat ggaaaagtgg cgtggtgaaa acaccaggcc ggagcagaag attattctga
     2641 tgatttactt tttcgtttcg ggcgtcggat aaacagccag ataaacgtaa ccacaggagc
     2701 cgaaggtgtc ggcttcgcag gttaaatcgt tgtggtacag ggtaacgcag tgggcatggt
     2761 gggggctgag ttcaccggtg gtcagcatcg attccatctg gcggataaag tgcgggaatg
     2821 tttcatccag cttccggcat tcgatgtcac tgaacttgcc ggtgatgctg gtccggtcag
     2881 ccagataatg cagtcggttg ccttcctgca ccagacgggc tcccaggcgc agtgtaatct
     2941 cccgctgcag gcccctggta gggttgctca ttacagttct ccactattgt cagttcaggg
     3001 tgatgc
//
LOCUS       DAOFQD010000252         2986 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.256, whole genome shotgun sequence.
ACCESSION   DAOFQD010000252 DAOFQD010000000
VERSION     DAOFQD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.256"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1461
                     /locus_tag="QBF46_004756"
     CDS             <1..1461
                     /locus_tag="QBF46_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676834.1"
                     /translation="KISDEQINIEAQSGYRPWTIDSGIGLLPFSELGDREFELLCYLL
                     VKNEIEDGKHKNISSISLMQGVSERGRDCVLYHNGVVSGLIQCKKMQGRITRPQALRE
                     IIKFLLFSIIDNSLLPAPDCFEYKLYVSNDIAETTNSLIHSYSTEVEVEIKSDTISKY
                     IKDVVSEYESFKIFENSLPISKVIDLLRRINVTASNATDLTLRVHKYDSLLSLFFNVK
                     TVIDLESADKVIRNALDDYGLKYLTDEDLKQLQIRISNTKEENRINLGFVDFFGYNKE
                     FFKSLKGEPFKQVIEAVASVTLSLDKYLMEFINSKINELVFENVTIKLLNPGRIHPFS
                     VGVVAPYLFQRLSIKVVSRKLPTELVTKFYEQASLSKKELIDSIAIQLFETSERVMKG
                     DFSQLVGNPEDIQFKKRIFTHIHNGFNSIEDAKTTFSRDIQIIMPTLDFIEEKIGELL
                     QEERTVVIKDASFLDNDDEIKKIAKTLSMIEPNPGA"
     gene            complement(2282..2623)
                     /gene="ykfI"
                     /locus_tag="QBF46_004757"
     CDS             complement(2282..2623)
                     /gene="ykfI"
                     /locus_tag="QBF46_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin YkfI"
                     /protein_id="HDQ6676835.1"
                     /translation="MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFC
                     NEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGFSWQEQSPYLRAADILRARQATG
                     LLRQSRNNVVR"
     gene            complement(2644..2961)
                     /gene="yafW"
                     /locus_tag="QBF46_004758"
     CDS             complement(2644..2961)
                     /gene="yafW"
                     /locus_tag="QBF46_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system antitoxin YafW"
                     /protein_id="HDQ6676836.1"
                     /translation="MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIE
                     CRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYHNDLTCEADTLGSCGYVYIAIYP
                     TQR"
BASE COUNT          917 a          545 c          655 g          869 t
ORIGIN      
        1 aaaatcagtg acgaacaaat taatatcgag gctcaatcag gatatagacc atggactata
       61 gattctggta tcggattgct tcccttttca gaattaggtg atcgagaatt tgagttgcta
      121 tgttatttgt tagtaaagaa cgaaattgaa gatggaaagc ataaaaatat atcctcaata
      181 tctttaatgc aaggagtttc tgaaagagga cgcgactgcg tgctatacca taacggtgtt
      241 gtaagtggtt taatacaatg taaaaaaatg caaggtagaa taactagacc gcaagcatta
      301 agagaaataa ttaaattttt attgttttcc attattgata atagtttgct tcccgctcct
      361 gattgtttcg aatataagtt gtatgtatca aatgacatcg ctgaaactac aaactcacta
      421 attcattctt actcaaccga agttgaggtg gaaataaagt ctgatactat cagtaagtat
      481 ataaaagatg ttgtatctga atacgagtca ttcaaaattt ttgaaaacag cctaccaatt
      541 agtaaagtta ttgatctttt gagacggata aatgtaaccg catcaaatgc aacagattta
      601 accttgcgag ttcataagta tgattcattg ttatcattgt tttttaatgt aaaaactgta
      661 atcgacttag aatctgcaga taaagttata cgtaacgcgc ttgatgacta tggtttaaaa
      721 tacttgaccg atgaagactt aaaacaactt caaattcgca tttcaaatac taaagaagaa
      781 aatcgaataa atttaggttt tgtggatttc tttggttata acaaagagtt ttttaaatcc
      841 ttgaaaggcg agccatttaa acaagtgata gaagcggttg cgtcggtaac attatcatta
      901 gataagtatt taatggaatt cataaatagt aaaatcaatg agctagtttt tgaaaacgtc
      961 acaataaaat tattaaatcc agggaggatt caccctttca gtgtgggcgt ggtggcacct
     1021 tacctatttc agagactgtc cattaaagtc gtctcaagaa agttgccaac agagttagtc
     1081 accaaatttt atgaacaagc atctctgtcc aaaaaggaat taatcgactc gatagcaata
     1141 cagctctttg aaacaagcga aagagttatg aaaggagatt tcagtcagtt agtgggaaat
     1201 ccagaagaca tacagtttaa aaagagaata ttcactcata tacataatgg attcaacagt
     1261 atagaagatg caaaaactac tttttctcga gacatccaga ttataatgcc aacattagat
     1321 tttatagaag aaaaaatagg tgagctatta caagaagagc gtactgtggt aataaaagac
     1381 gcctcttttt tggataatga tgatgagatt aagaaaatag caaaaacgct gagtatgatc
     1441 gaacccaatc caggcgcata gaataactgt agttttggta tttttatttt tagtagctgt
     1501 gttagtgtaa ttgcactaac acactccttg agctgctccc cgttgcttag gacacttcaa
     1561 tgttagtcat gaagtagtga acttccgctc atcgctcata acagacataa acttcagtta
     1621 tggcataaag gtatgcatgc tgggtgggga aagtatgaag gaaaagaaga ctgctgcgcc
     1681 gtttgtcgtc acgtttatct tcattggcta tgcaagtcgt aatacaaggt gggacaaaac
     1741 tgagacacat aaggcctcgc aatggcttgc aaggctttac atgttttgat gtggtgggac
     1801 gtgtgagcgc agtgttgatg gggtaatgct ttgaattaga agtggattct tataattcgt
     1861 aatgcgaagg tcgtaggttc gactcctatt atcggcacca ttaaaatcaa attgttacgt
     1921 aagatcttat cattctccca ccaaaaaatt atcttaatgt aacagctggt gtaagtaaat
     1981 tctatcaacg aagatcaatc ttatctactg accaaaaagg cctgataggg cttcgctcac
     2041 tatacatcct tggctgcagg tttagttgta caccactcct aaatttaatg tgttggcaat
     2101 gtgttcaata aagctcgaac aaattagctc attatgatcg gttaatactt caacttctgg
     2161 ttgcatgatt gtttgtccgt aaaaagataa cgcgcctgcc gggtagtagc aggcgcatta
     2221 cgcaataggt aaacaaggga ggaagttcag aaatgtaaat cgggaaggtt gtacgcaatg
     2281 ttcatcgtac tacgttgtta cggctttgcc gcaacaagcc agttgcctgc cgcgctcgca
     2341 gaatgtctgc agcccggaga taaggagatt gttcctgcca gctaaatccc ttcctgtcga
     2401 tacgaaccag ctcgtatttt tctaccagaa aattcacggc atcggctagg gtgataccgg
     2461 catcgatgtg ttccttaatc acagcctcat tgcagaatgg cgtgtcgttt attgtcagac
     2521 catagtgctg ttccagcaga cgtgtcagta acatttgcca gacagccacg ggtgacaggc
     2581 agggcttcac cgcccgctga gttgttgcag gtaaagtttt catgtttgct ctcgtgtagg
     2641 taattaacgc tgagtggggt aaatggcgat gtatacgtag ccgcaactgc caagggtgtc
     2701 ggcttcgcag gttaaatcgt tgtggtacag ggtaacgcag tgggcatggt gggggctgag
     2761 ttcaccggtg gtcagcatcg attccatctg gcggataaag tgcgggaatg tttcatccag
     2821 cttccggcat tcgatgtcac tgaacttgcc ggtgatgctg gcccggtcag ccagataatg
     2881 cagtcggttg ccttcctgca ccagacgggc tcccaggcgc agtgtaatct cccgctgcag
     2941 gcccctggta gggttgctca ttacagttct ccactattgt cagttc
//
LOCUS       DAOFQD010000253         2894 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.257, whole genome shotgun sequence.
ACCESSION   DAOFQD010000253 DAOFQD010000000
VERSION     DAOFQD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.257"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(72..965)
                     /locus_tag="QBF46_004759"
     CDS             complement(72..965)
                     /locus_tag="QBF46_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HDQ6676837.1"
                     /translation="MELFAITDSEIPTRIIKIDIDAPAQTVVENLFRTQRSEFINEDI
                     EEIDFCASYNVQDGEIFSINPFDDEIGIINAIERPDAVPVWDPDDVSVHYFKALFTGE
                     PASNGNPTQVWLQCFDRRQIINNEKSFFQVVTQPGNRFSVSTRPGFSLSDRLTAILVG
                     DKLLFKSFFMLRRFFNMEEYFNEATREDLDNFIGNDIFHVENAEDFMTFADSAIKKKV
                     SLIISSGILNDQPIENLIECAQKIGYQLGITNVNGDNKITMPNSKREVKQLLYFLDQG
                     YFNSIITNELMLTNSKRPIRI"
     gene            complement(975..1493)
                     /locus_tag="QBF46_004760"
     CDS             complement(975..1493)
                     /locus_tag="QBF46_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676838.1"
                     /translation="MLNGFARYALTASSVAPVCLTLAFLAYINDNYKILVSSILLAVV
                     SVVFCVFIIKQAQKYNPVTLKNLESASPADKEITNYFLTYLFPLISGPTTFMDWRLAL
                     FFYLSLFFYIKHSSSYSFNPILSIIFGFKFYEAEDDTGVSFVLLSTKPLTNAKIKGLR
                     VKKLTEHTYMIV"
     gene            1694..2107
                     /locus_tag="QBF46_004761"
     CDS             1694..2107
                     /locus_tag="QBF46_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676839.1"
                     /translation="MEKSKHDDQHRDRFRKDKNLRLLRHFCISVRLNQSELELLNNIR
                     GKHKKGEWLRLTFLNHTPTNIPLVNVEAWKMLSDISQKLNRIAIHIDGKSKDSKLTHT
                     ELFAVKRQLQELRQHLLNADIWSKPYEGNAEDQKG"
     gene            2076..>2894
                     /locus_tag="QBF46_004762"
     CDS             2076..>2894
                     /locus_tag="QBF46_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDQ6676840.1"
                     /translation="MKGMQKIKRGKSFSGVVQYALKPGSHHKSDPIVIGGNMLGDSAL
                     ELIAEFDSTKHLRQDVAKPVWHNSLRLPNGESLTAEQWSSIADDYMRRMGFGDTHLRC
                     YVLHDDSAGQHIHIIASRIDIAGGKLYLGRNENLISTRIISELEIAHGLTVTKTAPSI
                     TPKQQKRRKVSRNEQMLSERTGLPSPKEALQQILDKSLADTPDLLTFIKRLEEAKVGW
                     TANIASTGKMNGFSFEYRDIAFKASQLGKSYSWANLSNRLNNNPDHLEALRTNTP"
BASE COUNT          965 a          595 c          532 g          802 t
ORIGIN      
        1 caagagctaa ctcagcgaag ctgagtagtc ttgctttact tgctcttcga ttagtaaagg
       61 aaagcaaaat ctcaaattct tattggtcgc ttcgaattcg tcaacattaa ctcatttgtg
      121 attattgaat tgaagtagcc ctgatcgagg aaatataata gttgcttcac ttctctttta
      181 gagtttggca tagtgatttt attgtcacca ttcacattag ttatacctaa ttgatatcca
      241 atcttctggg cacactcaat tagattttca ataggctgat cgttgagtat tccagaactt
      301 attatcaaag atactttctt tttaattgca gaatctgcaa acgtcataaa atcttcagca
      361 ttttccacat gaaatatatc atttccgata aagttatcta aatcctctct agtagcttca
      421 ttaaaatact cttccatgtt aaaaaatcgc cggagcataa agaaactctt gaacaataat
      481 ttatcaccca caagaatagc agttaatcga tcactgagcg aaaaaccagg acgagtggac
      541 actgagaatc tatttccagg ctgagttact acttggaaaa aggatttttc attattaatt
      601 atttgtcgtc tgtcgaaaca ttgaagccaa acctgagtag gattaccatt cgaagctggc
      661 tcacccgtaa ataacgcttt aaaataatga actgacacat catcagggtc ccaaaccgga
      721 acagcatcag gccgttcgat tgcattaata atgcctattt cgtcatcaaa tggattgata
      781 gaaaatattt caccatcctg aacattataa gatgcacaaa agtctatctc ttcgatatct
      841 tcatttataa actcacttct ctgagttcta aatagatttt ctactaccgt ttgtgcggga
      901 gcatctatgt caatcttaat tattctagtc ggtatctcgc tgtctgtaat cgcaaaaagt
      961 tccatataat atccttatac aatcatatag gtatgttcag taagcttctt taccctcaaa
     1021 cctttgatct tcgcattggt aagaggtttt gttgacaaaa gaacaaagct caccccggtg
     1081 tcatcttcgg cttcatagaa cttgaatcca aatataatag acaaaattgg attgaaacta
     1141 tacgaagacg agtgttttat gtagaagaaa agacttaaat aaaaaaatag agctaatcgc
     1201 caatccataa aagtggtcgg cccagatata agtggaaata agtaagtcaa gaaatagttg
     1261 gttatttcct tatctgccgg agatgccgat tctaagtttt tcaatgtgac cggattatat
     1321 ttttgtgctt gtttgattat aaagacacaa aaaaccacac ttacgacagc caaaagaatt
     1381 gaggaaacaa gaatcttata attatcatta atataagcta agaatgccaa tgtcaggcaa
     1441 actggggcaa ccgaactagc agtcaaagca tagcgagcaa atccattaag catacaccac
     1501 cctccataac tgtataaaaa accagtatac atgaagtccg tggtcatagc gatcagcctt
     1561 agtgctaaat ttcatcgaac tcaatacata ttataaagtt cagattgaca aacacaattc
     1621 atttggtaga tgtattttat catcataatc aataagttaa ctcgattcac cattttttgc
     1681 ataaggaaat gctatggaaa aatctaaaca tgacgatcag catcgtgatc gatttaggaa
     1741 agataaaaat ctaaggttgt tacgccattt ttgtatcagt gtgcgtttga atcagtcaga
     1801 gctagagctt ttaaacaaca taaggggcaa gcataaaaaa ggggagtggt taagattaac
     1861 ctttttaaat cacaccccaa ccaacatccc gttagtaaat gttgaagctt ggaaaatgtt
     1921 gtcagacata tcgcaaaagc taaatcgaat tgcgatccat atcgatggta agagcaaaga
     1981 cagcaagctt actcatacag agcttttcgc tgtgaagcga caactgcaag agcttcgcca
     2041 gcacctacta aatgctgaca tatggagcaa accctatgaa gggaatgcag aagatcaaaa
     2101 ggggtaagag cttctctggt gttgttcaat atgcattgaa gccaggttca catcataaaa
     2161 gtgatccaat cgtcattggc gggaacatgt taggtgattc agcccttgaa ctgatcgctg
     2221 aattcgatag caccaaacat cttcgccagg atgtcgcaaa accagtttgg cacaactcgc
     2281 ttcgcttacc caacggtgaa tcactaacgg ctgaacaatg gtccagcatc gcagatgact
     2341 atatgaggag aatgggattc ggtgataccc atctccgttg ctatgtgctt catgacgatt
     2401 cggctggtca gcacatacac attatcgcca gcagaatcga catagctggt gggaagctct
     2461 acttgggtag gaacgaaaac cttataagca cacgaattat tagtgaactc gaaatcgctc
     2521 acggtctgac agtgaccaag acagcacctt ccattacacc aaagcaacag aagcgaagaa
     2581 aggtatcccg taatgaacaa atgctttcag aacggacagg ccttccctcc cccaaagaag
     2641 ctctccaaca gatacttgat aaaagtttgg cagatacacc tgacctttta acttttatca
     2701 aaaggctgga agaagcaaaa gtcggctgga cggctaacat tgcttctacc ggtaagatga
     2761 acggcttctc attcgaatac cgcgatatag cattcaaagc ttcgcaattg ggtaagagct
     2821 actcctgggc aaatctcagt aatcggctta acaacaaccc cgaccactta gaagctttac
     2881 gaaccaacac accg
//
LOCUS       DAOFQD010000254         2763 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.258, whole genome shotgun sequence.
ACCESSION   DAOFQD010000254 DAOFQD010000000
VERSION     DAOFQD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.258"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(159..1349)
                     /gene="aspC"
                     /locus_tag="QBF46_004763"
     CDS             complement(159..1349)
                     /gene="aspC"
                     /locus_tag="QBF46_004763"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDQ6676841.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1534..2622)
                     /gene="ompF"
                     /locus_tag="QBF46_004764"
     CDS             complement(1534..2622)
                     /gene="ompF"
                     /locus_tag="QBF46_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDQ6676842.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADIGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          685 a          682 c          667 g          729 t
ORIGIN      
        1 gccggatgcg gcacgagcgc cttatccggc ctacaaaatc gtgcaaattc aaaatattgc
       61 aggggacgcg taggcctgat aagcgtagcg catcaggcaa tgttgcgttt gtcatcagtc
      121 tcagcccgct tttcagcggg cttcattgtt tttaatgctt acagcactgc cacaatcgct
      181 tcgcacagcg gagccatgtt atctggtgtc atcccggcca catttacgcg accagaagca
      241 accgcatata cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt caggccactg
      301 aaggagaaca tgccgttctg tttgatgata aagctgaagt cgcggtttgc gcctttttcc
      361 tgcagcgtat tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc
      421 tcttgttccc aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg
      481 ccgtgtgctg gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg
      541 cgatcaacgg tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac
      601 aggccaaagt ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa
      661 gcgcgcagtc cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca
      721 aacagcggta accagccttt ctcaacggag agttgtgcca gtgtctgcca ttgttccagc
      781 gtagggtcga taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc
      841 tgagcttcat tcaggctgtt aatcagtgca tcgaagtcaa gagtgtgatt ttccgcatca
      901 taataagcgt attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc
      961 cagcttgggt tgctcaccca cacacgctta acgctggtat tttttgccag gaaatcggca
     1021 gccacgcgta gtgcgccagt gccccccgga gtctgtgccg tgcgagcacg tttgtcattg
     1081 atcagggcgc tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg
     1141 ccgtcaatgc cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt
     1201 ttcacgctgg tcagtaccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg
     1261 ttaattttgc cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg
     1321 gcaggagcgg cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa
     1381 gtccgctatc agggtaacgg gagatttaca aaattccaac tattactgat gaaaactcag
     1441 gctgtttttg caagacgtga gattgctctg gaaggtataa aaaaaacagg accaacgtcc
     1501 tgttttttcg gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc
     1561 aacggtgtcg tctgaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta
     1621 gtcaacatag gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt
     1681 caccagatca acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat
     1741 ggacggacgc agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt
     1801 gttggcgaag ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc
     1861 accgtagttc gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg
     1921 ttcagctttt ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc
     1981 accataagca ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc
     2041 gtcgccgtta gagcggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa
     2101 gttcaggcca tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac
     2161 acgaccaacg aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc
     2221 ggtgtaaccc agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaat
     2281 gtcagcgtat ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc
     2341 gccttcagag ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga
     2401 attgatttgt gtttcccctt taaaaccaag acgggcataa gttttgtcgc cgtttccgcc
     2461 gtaactgttt acaccattgt ctttagaaaa ataatgcaga ccgacagctt taccgtacag
     2521 atctactttg ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa
     2581 cagagcaggg acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg
     2641 ttttttttat gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac
     2701 tattgatgag agtttggtgt ctttatgtgt ctgcaggcat ctttccattc aaactaacgt
     2761 ttc
//
LOCUS       DAOFQD010000255         2735 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.259, whole genome shotgun sequence.
ACCESSION   DAOFQD010000255 DAOFQD010000000
VERSION     DAOFQD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.259"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="QBF46_004765"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="QBF46_004765"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDQ6676843.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2117)
                     /locus_tag="QBF46_004766"
     CDS             complement(1725..2117)
                     /locus_tag="QBF46_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDQ6676844.1"
                     /translation="MKRLRNKMTSEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLSRLDIDESA"
     gene            complement(2119..2478)
                     /locus_tag="QBF46_004767"
     CDS             complement(2119..2478)
                     /locus_tag="QBF46_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDQ6676845.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
BASE COUNT          677 a          657 c          687 g          714 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc acgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggagaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctgatccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcgga ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtgacgg
     2701 ggctatagct cagctgggag agcgcttgca tggca
//
LOCUS       DAOFQD010000256         2693 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.260, whole genome shotgun sequence.
ACCESSION   DAOFQD010000256 DAOFQD010000000
VERSION     DAOFQD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.260"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            223..882
                     /locus_tag="QBF46_004768"
     CDS             223..882
                     /locus_tag="QBF46_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HDQ6676846.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1083..1460)
                     /locus_tag="QBF46_004769"
     CDS             complement(1083..1460)
                     /locus_tag="QBF46_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6676847.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1771..>2693)
                     /locus_tag="QBF46_004770"
     CDS             complement(1771..>2693)
                     /locus_tag="QBF46_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS4321 family transposase"
                     /protein_id="HDQ6676848.1"
                     /translation="YRKKFTRPKLIEFLATCPATTIAMEACGGSHFMARKLEELGHSP
                     KLISPQFVRPFVKSNKNDFVDAEAICEAASRPSMRFVQPRTESQQAMRALHRVRESLV
                     QDKVKTTNQMHAFLLEFGISVPRGAAVISRLSTILEDNSLPLYLSQLLLKLQQHYHYL
                     VEQIKDLESQLKRKLDEDEVGQRLLSIPCVGTLTASTISTEIGDGKQYASSRDFAAAT
                     GLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVFIQKLEHQSGKLADWVRDLLCR
                     KSNFVVTCALANKLARIAWALTARQQTYVA"
BASE COUNT          689 a          633 c          627 g          744 t
ORIGIN      
        1 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
       61 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt
      121 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      181 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      241 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      301 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      361 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      421 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      481 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      541 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      601 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      661 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      721 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      781 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      841 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
      901 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
      961 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     1021 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     1081 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     1141 tgatcattta ttctgcctcc cagagcctga taaaaacggt tagcgccggg gttggatttt
     1201 tcagcgttcc agctaaggct aaggcattcc tgttcaagcg caagccgggc tataaagcgc
     1261 attatcgctt tacccgtgcc cttatttcga tcgcactgag aaacatacag ctctttaata
     1321 tgcagctgac cgctgtatcg gggcgaggga taaagaatat tgcaacatgc caggccagta
     1381 atgttattgc cgcagcgcgc tctgatcacc agggtaccgg aaagccggtt gaataacttt
     1441 ttacagagat aatccttcat caacgcttcc tgaatgatac cttcgccata ataggggtcg
     1501 tctcagaaaa cggaatctat ggtcactccc gtttttgcaa caccgatttt gacgacaagt
     1561 tggcttgctt gaatctatcc ggcgtctgaa tgggatttta ttcccgcgcc ttgatgagtt
     1621 ccgcgcctga tgaacctcca gaaaatatac ggcttcaatg agcctttccg ttttacaggt
     1681 tcctcaacag gccggtgggc cgttagtatc atcaatatca gtattcgcaa aaccagatca
     1741 gtaattcttt aaaccggtgt atttctgccg ttatgctaca taagtttgct gtcgtgccgt
     1801 tagggcccag gctattctgg ccagcttgtt tgccagagca caagtgacga caaagttgct
     1861 tttccggcac agtaaatccc tgacccaatc ggccaatttg ccagactggt gttccagttt
     1921 ttgtatgaat accctggcac attgaaccaa caaagttcgg atctttttat tacctcgctt
     1981 actaattccc agcaatgtcg tcctacctcc cgtgctgtac tgccgaggta caagccctgt
     2041 tgccgccgca aagtcacggc tgctggcgta ctgcttcccg tcgccaatct cagttgaaat
     2101 agtactcgct gtcagtgttc cgacgcaggg aatgctcagc aagcgctgtc caacctcatc
     2161 ttcgtccaac tttcgtttca actgggattc caaatcttta atctgctcaa caagatagtg
     2221 ataatgctgt tgtaatttca gcaataactg gctgaggtaa agaggcaaac tattatcctc
     2281 aagaatggta ctcagtcggc taataacggc agctcctcgg ggaacgctaa tgccaaattc
     2341 cagcagaaaa gcatgcattt gattggttgt ttttacctta tcctgaacca gggattcacg
     2401 gacacgatgc agagcccgca ttgcctgctg agattccgtt ctgggctgca caaaacgcat
     2461 agacggacgc gatgcagctt cacaaatagc ttcggcgtcg acaaagtcgt ttttattgct
     2521 tttaacgaac gggcggacaa attgtggtga tatcagcttt ggggaatgcc ccaactcttc
     2581 caacttgcgt gccataaagt gagaaccgcc acaggcttcc attgcgatgg ttgtagcggg
     2641 gcatgtcgcc aaaaattcga tcaactttgg ccgggtaaat tttttacggt aaa
//
LOCUS       DAOFQD010000257         2668 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.261, whole genome shotgun sequence.
ACCESSION   DAOFQD010000257 DAOFQD010000000
VERSION     DAOFQD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.261"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            84..809
                     /locus_tag="QBF46_004771"
     CDS             84..809
                     /locus_tag="QBF46_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDQ6676849.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            767..1444
                     /gene="bglJ"
                     /locus_tag="QBF46_004772"
     CDS             767..1444
                     /gene="bglJ"
                     /locus_tag="QBF46_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDQ6676850.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1482..2270)
                     /gene="fhuF"
                     /locus_tag="QBF46_004773"
     CDS             complement(1482..2270)
                     /gene="fhuF"
                     /locus_tag="QBF46_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDQ6676851.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHAIFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2411..2647
                     /locus_tag="QBF46_004774"
     CDS             2411..2647
                     /locus_tag="QBF46_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDQ6676852.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYILLPSCLALSAGIMLMLMNLNQG"
BASE COUNT          700 a          582 c          673 g          713 t
ORIGIN      
        1 tctattaatt tgctggaaac atttaaggaa tgaatatcct gaataaacca tcataatttt
       61 tagacagtgg atgtggagga aatatgttgc caggatgctg caaaaatgga attgttatca
      121 gtaaaatacc tgttatgcaa gcagggttaa aagaggtcat gaggactcac ttccctgaat
      181 atgaaataat atccagcgcc tctgcggagg accttacctt attacaatta cgtcgttccg
      241 gattagtcat tgctgattta gcaggtgaaa gtgaagatcc acgttctgtt tgtgaacatt
      301 attattcttt aatctcacaa tatcgggaaa ttcactgggt tttcatggtg tcacgctcct
      361 ggtattccca ggcagtagaa ctgctcatgt gccctacggc gacgttattg tctgatgttg
      421 aacccattga gaatctggtc aagaccgtac gttccggcaa tacgcacgca gagcgtatca
      481 gcgccatgct gacctccccg gcaatgactg aaactcatga ttttagctat cgctccgtca
      541 ttctcactct ttcagagcgc aaggtactgc ggctattagg taaaggatgg ggcatcaacc
      601 agatagcttc attgcttaag aaaagtaata aaactatcag cgcccaaaaa aacagtgcga
      661 tgcgtcgact ggcaattcac agcaacgctg aaatgtatgc atggataaat agcgcgcagg
      721 gtgcaagaga acttaacttg ccttctgttt atggagatgc cgcagaatgg aacacagccg
      781 aattaagaag agaaatgtcg cactcataga aaaatgcgtc atgagtagta tcggtattga
      841 gagtttattc agaaagtttg cgggcaaccc ttataagctc catacctata ccagtcagga
      901 gtcatttcag gatgccatgt cgcggatctc gtttgcggcg gtcatttttt ctttttctgc
      961 catgagaagt gagcgcagag agggattatc ttgcctgact gaactggcga ttaagtttcc
     1021 gcgtacccgg cgtttagtta ttgcggatga tgatattgaa gctcggctga ttggttcatt
     1081 gtcgccatca ccgctggacg gtgtattaag taaagcgtca acgctggaga tttttcatca
     1141 ggaactcttt ttgtcattaa atggtgtacg tcaggcgacc gaccgactga acaatcagtg
     1201 gtacattaac caaagccgga cgctaagccc gacggagaga gaaatattgc gctttatgtc
     1261 gcgtggctac tcaatgacac aaattgccga gcagcttaaa cgcaatatca aaacgatccg
     1321 cgcgcataaa tttaatgtga tgtcgaaact gggcgtcagt tctgatgcag gattgttgga
     1381 ggccgcagat attctgttat gtatgcggca ttgcgaagca agtaatgtgt tgcatcccta
     1441 ttaatccgca tgatgccggg tttacttccc cggcagtgct ttcatttcag cgtacaatcg
     1501 ccacattgct gcacatccgg taagcgataa cgctggcagc aagtgcggcg caccagcagg
     1561 ccgtcgcgca gtaccacggt acgccagagt ggattatctt caccgttcgt gagcgttttc
     1621 tcaaaaaaga tggcatggcg cagcgattca accgtcgcct cgccgagcag ttgcttcatc
     1681 tcagtgagat accagttgat caaataaccg gtattactcc agataagttt gccgttgatc
     1741 tctccggtcg cttctagtgc ctgcacaacc ggaaccagcg cctggctgat taacgtttcc
     1801 attcgctgct gcggcgaatg tggtgttgcg tttttatctt cacacacatc gacccagaaa
     1861 caggcggcac ggccggtttc gtgaaactca gcatggaaat gttccggcga cacatctaat
     1921 gctttttcct gcgtcagtag cgccagcatt aatggtggca ccatcaggcc gatataccat
     1981 tgtgcccata gtgagatcag cggtttgttc tcgcggatca tcgttggttg gttgcgatag
     2041 atatgatcgg aatagaccgc gagtagagaa cttagcgcat tcggtgatga ccattgcgcc
     2101 agcgtcatgg cgttaagcgg agcgggttca tccaggcgga taaactccag caaatgttca
     2161 cgatgttcgg cgatcgtcgc acgtacggct tgtgcaagcc cagcatcctg tggctggaga
     2221 tgcgttcgcc agatgatatc ttcatagagc ggtgcggaac gataggccat aatcgtgata
     2281 gtaatctaaa tgataatgat tgctaatcct agcgataggt ttacccgata gcaagggatt
     2341 tctctggctt gcaaatgata aaaattatca tatgatatcg gttatcatta tcaatgaaag
     2401 agatgaaacc atgttgcaac gtacgctggg cagtggctgg ggagtgttgc tgccgggatt
     2461 gctgattgca gggctgatgt atgcggattt atcgccggat cagtggcgga ttgtcattct
     2521 gatgggattg atattgacgc cggtgatgct gtatcacaaa cagttacggc attacatttt
     2581 gctaccatcg tgcctggcgc ttagtgctgg catcatgctg atgctaatga atttgaatca
     2641 gggatgaaaa atcaaggaag aaaagata
//
LOCUS       DAOFQD010000258         2656 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.262, whole genome shotgun sequence.
ACCESSION   DAOFQD010000258 DAOFQD010000000
VERSION     DAOFQD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.262"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(294..713)
                     /locus_tag="QBF46_004775"
     CDS             complement(294..713)
                     /locus_tag="QBF46_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676853.1"
                     /translation="MKYCYLIILSLTISSAQAETFPAKLTCNTGEQITLKVTGPEQRL
                     IYTSSLYGTFHGYLDVFKGLSPERQYEVLANIHGIARYRDSPLHFTLLMKRTNGEMDN
                     GSIIYRYTYLKDEQLIDEEGHCSPIKDGGFIEGSKSY"
     gene            1218..1991
                     /locus_tag="QBF46_004776"
     CDS             1218..1991
                     /locus_tag="QBF46_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676854.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAATDDDELLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASQAEVILATVRGIVRGVFIADKWLKSTRENFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(2052..2606)
                     /gene="pinE"
                     /locus_tag="QBF46_004777"
     CDS             complement(2052..2606)
                     /gene="pinE"
                     /locus_tag="QBF46_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HDQ6676855.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCVGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIASGVP
                     RQKVAIIYDVGISTLYKKFPVGDK"
BASE COUNT          769 a          569 c          575 g          743 t
ORIGIN      
        1 tgacctactc cctacattgt aacaggggat gatgataagg tgtatctttc gagctataag
       61 cacgataaat aagtgattaa aaatgactct ttaaactatt ttaatttaaa aaattaattg
      121 ttagttctaa ttatggtatg agggcatttt cggcatagtt ctaaccatat actttggtaa
      181 aaatgactgt tacggaacag accatagtaa tgcctgctat tggcacggag cgggctgcct
      241 gagcgccgtg gggtctgcaa acggtaagca taccgataac tatacgcttg gccttagtaa
      301 cttttgctcc cctcaataaa tccgccatct tttatcggac tgcaatgtcc ttcttcatca
      361 atcagttgct cgtctttcag gtaggtatat cgatagataa ttgagccatt atccatctca
      421 ccattggttc gtttcattag gagagtaaaa tgtaacgggc tgtccctata tcgagctatt
      481 ccatgaatat tggcaagtac ctcatattga cgctcaggtg ataacccttt aaatacatcg
      541 agatatccat ggaatgttcc atagagtgaa gaagtgtaga tcagtcgctg ctctggacca
      601 gtaactttaa gagtaatttg ttcacccgta ttacaggtta gcttggcggg aaatgtctca
      661 gcctgagcag aagaaatagt caagcttaaa attatcaggt agcaatattt catgtgatta
      721 gaatgtccaa aactaacatg tatactcggg attctataac tagttggatg tagacccaag
      781 atgtattgga ctattcttcg gttgtagttg gtcaaatcat atattttatg tcagcaatgt
      841 ctacttttgg cacatagcag accagagaca ctggcgtaaa gccatggagg atcggtggga
      901 ggaggtaaaa atcctctcat gcaaaaaata cgcaaaatcg ataacagttg gaaatcattc
      961 aatactcgca ctatcggaag ttcaccagcc agtcgtagca cgttcttgca tatgacgtgg
     1021 ctacgggttt cgagaccgac ccgatcatca aacgaaacat aaaattagct cacattatga
     1081 ggaaaggtat ctttttgcgc tatgtaaatt caaagggtta gcctcatttt cccgatggat
     1141 ttctcaacac tactagttgt gagcccttgc aatgttcatt aatatacgtc tcacaaataa
     1201 ttcatagata ttgcaaaatg gatattactg agtttccttc tggagtaatt gaacaccttg
     1261 gctggtatgt ataccgattg attgatccga gggacggaag caccttctat gtagggaaag
     1321 gcaaaggtaa ccgcgtattt gcccatatgc gcggtgaagt ggcagcgact gatgatgacg
     1381 agttactgag caacaagcta aagcaaatta gagaaataag attagcggga cttgaagtta
     1441 tccatgtcat ccatcgacat ggaatgactg atgaaaagac ggcgtacgaa gttgaagcag
     1501 cacttattga tgcctaccct gggttaacga atatcatgaa tggtgctggc agcaatgaat
     1561 tcggcgccgc gcatgtcaaa gagttgatag caacatatca acccgaaacc ataacatttc
     1621 atcataaagc attaatgatt tccgttaaca gaagtgcaaa ggattcagag ctttatgatg
     1681 cggttcgatt tagctggcgc attaatgtct ctcgcgccag ccaagcagaa gtcattcttg
     1741 ctactgtaag ggggatcgtt cgaggggttt tcattgctga taaatggctc aaatcaacac
     1801 gtgaaaattt ccctacgatg aaatactggg acgaggatcc ggactttgag gcaacacaaa
     1861 gttctcgcta tggttttgaa ggtcgagaag ccccacctga aatagcaaat ctttatcttg
     1921 gaaaaaaaat accagatgaa ttaagaaaaa aaggagctat gtccccggtc cgttactcac
     1981 ctaatttttg agtctttaag tgataagcat aaaccgcagc acgatcttct tgcatacgac
     2041 gtgctacggt ttcatttatc tccgaccgga aacttcttat acagtgtcga tataccaaca
     2101 tcatagatga tcgccacctt ctggcgagga acgcctgatg caattaatcg cccggcctgc
     2161 gcccattgtt ctggtgtaag tttgggacga cgtccaccaa ttcgtccctg tgcgcgagca
     2221 gcttccagtc cagcttttgt tcgttcaaca atcagttctc gttccatttc agccagggca
     2281 cccatgacat gaaaaaagaa acggcccatt ggggtactgg tatcaatact gtcagtcagg
     2341 cttcggaaat tcacgccacg ctggcgcaac tcttctatca gcgtaacaag atgccgcata
     2401 ctgcgcccta acctgtccag cttccagaca accagagtgt cacctgccga taatgtcctg
     2461 agcagttttt tcagtccggg cctttcggac ttcgtaccgc ttatcttgtc ttcaaaaatc
     2521 agttcacatc ctacacagtt caacgcatta cgttgtagat cggtgttctg gtcatttgtt
     2581 gatacgcgta catagccaat aagcatggtg gatctccctg acaaaagcag gaatgatgcc
     2641 atttgctcgt tatttc
//
LOCUS       DAOFQD010000259         2652 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.263, whole genome shotgun sequence.
ACCESSION   DAOFQD010000259 DAOFQD010000000
VERSION     DAOFQD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.263"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            628..1065
                     /locus_tag="QBF46_004778"
     CDS             628..1065
                     /locus_tag="QBF46_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676856.1"
                     /translation="MHKINYSDDIDLSKTSRRGSFNKSKIPSEAVRSYCVSVRLNVEE
                     LQLLNIKRGSYKKGEWLRMASLQKLPPVIPTINTKAWKALTDISQKLNRIATHIDCKS
                     KDSQLTHTELFAVKRQLEELRTNLLSDSIWSLPNEGYAADSQG"
     gene            1034..2182
                     /locus_tag="QBF46_004779"
     CDS             1034..2182
                     /locus_tag="QBF46_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDQ6676857.1"
                     /translation="MKGMRQIRRGKNFAGVVQYALKPGAHHKCDPVVIGGNMLGDTAH
                     EMIAEFDSTKHLRQDVAKPVWHNSLRLPDGESLSNDQWARIADDYMKRMGFSDTHVRC
                     YVLHDDPAGQHIHIIASRIDLNGGKLYLGRNENLISTRIISELEIAHGLTVTKPAPSI
                     TPKQQKRRKVSRNEQMLAERTGLPSPKEALQQILDKSLADTPDLLTFIKRLEEAEVGW
                     TANIASTGKMNGFSFEYRDIAFKASKLGKAYSWVNLQKQLNYNPDHLEALRTTKGVIP
                     APAPAPAPAPAIVLKTAERKESIGKKIAELELRLREDKRNEIVEKILQKNAAKQQKHL
                     RLTGWIPFLQRLIELLRKYGKSILHKVPTNFSEVYSTHHLKPTRKIRL"
BASE COUNT          889 a          554 c          497 g          712 t
ORIGIN      
        1 atgacagaca taaagaaaac caggtaataa agcaaaagaa attgaatgct gaacccaaaa
       61 atgatggacc ttcggcttct gttatgaata atacaccatc aaatactgca ataccagact
      121 taataactca caataacttt tattacaaat tagttctatt tgtagcgatt ggaatgacaa
      181 gtgcaccttg ggtttttatt tatttacgcg actatattta acttgataaa gaacccatcc
      241 taatgaaaaa atcactatgt aaaaatccat catgcctaac caaccagagt acttcggaaa
      301 aaaaaccaat acatttaaat tgattgaagc aacaagtgca aggaaaaaag ctatcttcat
      361 ataccctcct cattattaat aaatacagtt atagcaattt caatctcaaa gtcaacaatc
      421 cctctaaata taagcaagag ctaatttata gaagcggttg caccgttcta taaagctctt
      481 gcttttcttg cttcctttca gtcagacaat aaaagaaatc aaaacaatcc atcctataga
      541 ttgacaaaaa actcattcgt tatataagtg atatataaag catatggatt atgctaacta
      601 attaatatca tacaaaatgg agtttatatg cataagataa attatagcga tgatatagat
      661 ctatccaaaa cttcccgccg tggtagtttt aataaaagta aaattccttc agaagcagta
      721 agaagctatt gtgtcagtgt ccgattgaat gttgaagaac tgcaactcct taatataaag
      781 cgaggctctt ataaaaaagg cgagtggctt cgcatggctt cccttcaaaa gttgcctcct
      841 gttataccaa cgattaatac caaagcctgg aaagctttaa ctgatatatc gcaaaagcta
      901 aatcgaattg cgacccatat agattgtaaa agcaaggaca gtcagcttac ccatactgaa
      961 ttgttcgctg tgaagcgaca actggaagag cttcgcacga atcttttaag tgacagtata
     1021 tggagcctcc cgaatgaagg gtatgcggca gattcgcagg ggtaagaact ttgctggtgt
     1081 ggtccaatac gcattgaaac cgggagcaca tcataaatgc gatccagtcg taatcggggg
     1141 caatatgctg ggtgacaccg cccatgaaat gattgcagag ttcgatagca ccaagcatct
     1201 tcgccaggat gtcgcaaaac cagtttggca caactcgctt cgcttacctg atggtgaatc
     1261 attatcgaat gaccaatggg caaggatagc tgacgactac atgaagagaa tgggattcag
     1321 tgatactcat gttcgttgct atgtgcttca tgacgatccg gctggtcagc acatacacat
     1381 tatcgcaagt cgcatagacc tcaatggagg gaagctctac ttgggtagga atgaaaacct
     1441 tatcagcaca cggatcatta gtgaactcga aatcgctcac ggtctgacag tgaccaagcc
     1501 agccccttcc attacaccaa agcaacagaa gcgaagaaaa gtttcccgta atgaacagat
     1561 gcttgcagaa cggactggcc ttccctcccc taaagaagct ctacaacaga tacttgataa
     1621 aagtttggca gatacacctg atcttttaac ttttataaaa cgactggaag aagctgaagt
     1681 cggctggacg gctaacattg cttctaccgg taagatgaac ggcttctcat tcgaataccg
     1741 cgatatcgct ttcaaggcat ccaaacttgg aaaggcatac tcttgggtaa atcttcaaaa
     1801 gcagcttaac tacaacccag atcacttaga agctctgcga acaacaaagg gagttatccc
     1861 tgctcctgct cctgctcctg ctcctgctcc tgcaatagtt ttgaaaaccg ctgagagaaa
     1921 ggaaagtatc ggtaaaaaaa ttgcagaact ggaactacgg ctcagagaag acaaacggaa
     1981 cgaaatcgta gaaaagatcc ttcaaaagaa tgcagccaaa cagcaaaagc atctcaggct
     2041 caccggctgg attccctttt tacaacggct catagagctt ctcagaaaat atggaaagag
     2101 catcctccac aaggttccta caaacttctc agaagtctat tcaacacatc atctgaaacc
     2161 tacaagaaaa attcgtttat agacagctag ttgtaaacac ctttcgtaac ctaactcaat
     2221 tgcttagtaa taaatttgtg tttagtcaag aaaatattga tcgttattac agatcgataa
     2281 tctagctatg atagacaata actatcaagt aacaatcatc gatcggtaca agcaatttta
     2341 ctaaagcgag tcggtcatcg agaatcccta caaacagcag taaatcttcc accctggggt
     2401 tgctatgaaa aacgaaacaa ttaagtgccc gttctgcttt caggagagtc cgtttggtgt
     2461 ccgtgtctgc actggctgtc attcaactat tagatatgga gccgtttcag cctggttagc
     2521 tttactaata ggaattgtgc tgttagcgat tgcttttctt atattcgcta tcagtcaaaa
     2581 ttttatatta actgtcgtta tctattttgc gtcagcaatt tctttaagat tttttttacg
     2641 taagaaattt gc
//
LOCUS       DAOFQD010000260         2592 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.264, whole genome shotgun sequence.
ACCESSION   DAOFQD010000260 DAOFQD010000000
VERSION     DAOFQD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.264"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            352..1605
                     /locus_tag="QBF46_004780"
     CDS             352..1605
                     /locus_tag="QBF46_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDQ6676858.1"
                     /translation="MNLIDKIALVGQRMKSEQISLKESLLVSSRVSVSDDSVDGVDRL
                     IYNHCLNKKNLSDFFGKSRVTFNKILADLEEKELVGAPIYQNKNHLYTRWDVQKIMDA
                     LNYPRYSDYYCSRTIVTQNHKGGTGKSTTSGVLAVAAALDLHLNARILLIEWDPQGSI
                     GSGMIQSVAEDDVFLTAIDAILGVYEEDSDYRKYLDLGYSEEQIIEGMPFSTHLPNLD
                     VITAFPTDARFKDKYWQCSREERTELLLRFKEVILPVLKSKYDLIIIDTPPEDSPITW
                     AADEAADGILVAVSPREYDYASTTDFMLTISERFKQSPSKGENLSWFKVLAVNVDDKS
                     PYEKIVLDKLVRTVQELFMSANIKNSEAFKAAASRGRTVLDIKKSEELCSPKQLDVAE
                     ESVMAVYQQFINEIKSFSVKQGGNV"
     gene            1602..>2592
                     /locus_tag="QBF46_004781"
     CDS             1602..>2592
                     /locus_tag="QBF46_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HDQ6676859.1"
                     /translation="MSDEQHIGNDKSRYINAPKRTEVIHRSGLQGLKGQPRLKKLFTL
                     HNGRKLEAEHIIVPAEKVELETTVHPLNPRNQEALSVNAVRDILKDIEARGVDTEGVA
                     VKRNGVYLLIEGSRRRFCCIQSAKELPLWVLPDDVNADDINSIISATQTSRRFSYREV
                     GLKYLREMEEHGFVTNEELANYHGISHVSVSKRVQAAKINSTLIALFPDYEAIPNSYY
                     NRLFRLQKYIEKNLFSLEEVVENTREEIRDLDISDIAEAQKTVMEKITTVVEKLDIKP
                     PSKGWDTRELATFTNKDKYARISKSSSGRKIRFEFNRMNRELIDEIEKFIKSKL"
BASE COUNT          814 a          482 c          590 g          706 t
ORIGIN      
        1 atgctggtct cgtaccatgg catttaaccc ccttaatcgc caggctatgg ggtaatgctg
       61 gtcgggtacc gtgggattta acccccttat tgccacatct atggagtgat cctgctatgt
      121 cggaagattg gtattgttga tacttaagag gcttttggaa gacttcaatg aactgccaat
      181 tgaaattcaa taatgcagat attccaaaat ctaagaaaag aatacagaac gttagcgtaa
      241 taatagaatt aaggttacta ttaaacgcat aacgctaaag tatacgcatt atgcgtaaag
      301 tttacgtata tacgtttaat ttcactattt aggtaaaggg atcggaagag aatgaatcta
      361 attgataaga tcgcccttgt cggtcaacgc atgaagagcg agcagatctc tctgaaagag
      421 tcattattgg tctcttcgag ggtttcggta tcggatgata gtgtagacgg tgttgatcgc
      481 ctaatctata accactgctt gaataaaaaa aatctgtcag atttttttgg taaatctcgc
      541 gtaactttta acaaaatctt agcggatctt gaagaaaaag aactggttgg tgctcctatc
      601 taccagaaca aaaaccatct ctatacccgc tgggatgtcc agaaaataat ggatgctctg
      661 aactatcctc gatatagcga ctactactgt agccgtacaa tcgtaacgca gaaccataag
      721 ggtggtacgg ggaagagtac aacttcaggc gttcttgcag ttgcggcagc acttgatttg
      781 catctaaacg cgcggatact gttgatcgaa tgggacccac aagggtctat tggaagcggg
      841 atgatccaga gcgtagcgga agatgatgtt ttcctgactg ctattgacgc catcttaggg
      901 gtttacgaag aagactcaga ttacagaaaa tatcttgatt tagggtactc ggaagaacaa
      961 attattgaag gtatgccttt ttcaacacac cttccgaatc ttgatgttat taccgcattc
     1021 cctacagatg cacgtttcaa agataaatac tggcagtgtt ccagagaaga acgaactgag
     1081 ttattactac gttttaaaga ggtaatcctg ccggtcttga agagcaagta cgacctgatc
     1141 attatcgata ctccaccaga agattcgcca atcacatggg cagcggatga agctgctgat
     1201 ggcattttgg ttgccgtttc acctcgtgag tatgattatg cctcaacaac tgatttcatg
     1261 ttgacgatca gtgagcgatt caaacaatcg ccgagcaagg gcgaaaatct aagctggttc
     1321 aaagtgttgg cagtgaatgt cgacgataag agtccctatg aaaagattgt cctggataaa
     1381 ctggtaagga cagtgcagga actctttatg tctgcaaata tcaaaaactc agaagcgttt
     1441 aaagccgcag cttctagggg tagaaccgta ttggatatca aaaaatcaga agagctctgc
     1501 tctcccaaac agcttgatgt tgccgaagaa tccgtgatgg cggtctacca gcaatttatt
     1561 aatgagatta agagtttttc agtgaagcaa gggggtaatg tatgagtgat gagcagcata
     1621 tcgggaacga caaatcccgt tatattaatg cgcctaagcg tactgaagta atccatcgct
     1681 ccgggcttca aggcctgaaa ggtcagccac gcctgaagaa actttttaca ttacacaatg
     1741 ggcggaagct tgaggcggaa cacatcattg ttccggcgga aaaggtggaa ctggaaacca
     1801 cagtacaccc tctgaaccca cgaaatcagg aggctctttc tgtaaatgca gtaagagata
     1861 ttctgaagga cattgaagca agaggtgttg atactgaggg cgttgcagtt aagcggaacg
     1921 gagtgtatct gctaatagaa ggtagccgca ggcgtttctg ttgcattcaa tcagcaaaag
     1981 aattgccatt atgggttttg cctgatgatg ttaacgcaga tgatattaat tccatcatat
     2041 cagcaacaca aacttctcga cgcttttcat atcgtgaagt aggtttaaag tatttaaggg
     2101 aaatggaaga gcatggattt gttaccaatg aagagcttgc aaactatcat ggtatcagtc
     2161 acgtgtccgt ctctaagcga gttcaggctg caaagattaa cagcactctt attgcacttt
     2221 ttcctgacta tgaagctatt ccgaattctt attacaatcg cctattccga cttcaaaaat
     2281 atatagagaa gaatttattt tctcttgaag aagttgttga gaatacacgt gaggaaattc
     2341 gagatctgga tatttctgat attgctgaag cacagaaaac cgtaatggag aagattacaa
     2401 cggtagttga aaaacttgat attaaaccac ctagcaaagg ttgggataca cgtgaattgg
     2461 caacttttac taataaagat aaatatgcaa gaattagcaa aagttcctcg gggcgtaaaa
     2521 tccgttttga gtttaataga atgaaccgag aattaattga cgaaattgag aagtttataa
     2581 aatcaaaact ga
//
LOCUS       DAOFQD010000261         2577 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.265, whole genome shotgun sequence.
ACCESSION   DAOFQD010000261 DAOFQD010000000
VERSION     DAOFQD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.265"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            140..325
                     /locus_tag="QBF46_004782"
     CDS             140..325
                     /locus_tag="QBF46_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDQ6676860.1"
                     /translation="MKSTKSRYRHVKKKLGRKEGSKYRRDITILKKGGFTQAEIAKKL
                     SISLSTVKRHWNNGIIG"
     gene            597..1223
                     /locus_tag="QBF46_004783"
     CDS             597..1223
                     /locus_tag="QBF46_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDQ6676861.1"
                     /translation="MKTYQGTHGVHILEYQSKINKLLCYLTNRYPRLIAVRVDLHYPK
                     IVDNGDNICCFPNLEPGVISRMRESLRAKLEADRTRKVREDKRIYRCPLFIIWAKEYS
                     ESGKCHYHICLLFNKDAYYHLGDYDQDDNLRGMITGAWYSALRLERDDHPGLVHFPEN
                     CKYVLDTNSTDFQQNYQALLTRLDYLTKVESKVFGEGDRNFGCSQIEL"
     gene            complement(1314..>2577)
                     /locus_tag="QBF46_004784"
     CDS             complement(1314..>2577)
                     /locus_tag="QBF46_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HDQ6676862.1"
                     /translation="ITVNTLFKGDDTSPPSGRILVNPVEMKPNPAASAEVWEAFEALP
                     SQTRPPKGARPARRLTALAQELVDDGILDGAVRKAHAALHAVLDKFQIENAEKIKNKR
                     NAVLVVDGKAVVASLKNKAMTFNEFWEEADVTVIDDAYRRAARIFSPDVSRTYVEYLA
                     DKVADREEDAEEYLEAIMEARVTVAGMGLVMEVQDYFDGEADRLAKAWLAEYTPQIKS
                     LKDERKEAYRQIVEMSTEPQDVDLVRPANKFEMTRVREGEKEADLPVWKHHLLCDESG
                     NYPALLNHWETKVFEIETKREGFAFWYRNPQYTGQSSLGIAYVEAEQYKIVRPDFLFF
                     AEQDGKMVVDLVDPHSLHLADALPKLEGLALYAEHHSDAYRRIESVAEVKGKLRVLDL
                     KRQDVQDAVATAENAETLFSSGLADDYQ"
BASE COUNT          691 a          631 c          560 g          695 t
ORIGIN      
        1 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac cctaccatcc
       61 agcttcaagg atagagcctt ctgctgaaag ttgcttgtct tttttgattg ctcgtcagga
      121 tacgattgac attcacaatc tgaaatcaac taaaagccga tatcgacatg ttaagaagaa
      181 attaggacga aaggagggaa gcaaataccg acgtgatatc acaatactta agaaaggtgg
      241 atttactcag gctgagattg caaagaaatt gagtatcagt ttatcgactg tgaagcggca
      301 ctggaataac ggaatcatcg gttaattcca gaagctaatt gcagagtaac tttttaacag
      361 ccatcacagg ctggggctac ctccgtctca aatttagatg ttggataggt attatataat
      421 ggcattgtgt actcttacta cctgtattca ccagtaagag tagtaatcag atcagtatgt
      481 ccgtactaaa atctgatacc agtatatacc gacaacagtg accaggaata gccctgcctg
      541 tatggcatcc ctgtacggtc tgctatagac cgtaggacca aatacaggta tatattatga
      601 aaacatatca aggtactcat ggagtacata tcctcgaata tcagagtaag attaataaat
      661 tattatgtta tttgactaac cggtatccta ggttaattgc tgtacgtgtc gatctccact
      721 atcccaaaat agttgataac ggtgacaata tctgttgctt ccctaacctg gaacctgggg
      781 tgatatcccg gatgcgtgaa tccctcagag ctaagctgga ggctgaccgt acccgtaagg
      841 tgcgtgagga taaacgcatt taccgttgcc ctttatttat tatatgggcg aaagagtatt
      901 ctgaatcagg taaatgccac tatcacattt gcctgttgtt caacaaagac gcttactacc
      961 acctcgggga ttacgatcag gacgataacc ttagagggat gatcacagga gcctggtaca
     1021 gcgccctgag actggagaga gacgatcacc ctggtctggt ccattttcct gaaaactgta
     1081 agtatgtgct agacaccaat agtactgact tccagcagaa ctaccaggcg ctgttaaccc
     1141 gactggacta cctgaccaaa gttgaaagca aagtgtttgg tgaaggagac cgtaacttcg
     1201 gctgtagcca aatcgaactg taagtcggta atggccttct cagtgagaag gccttatttg
     1261 tattgtttca aaaaattact aaaatgcagt ttcgttacgc aattttctat agattactga
     1321 tagtcatcag caagtccgct actaaataac gtttctgcat tttcagcggt agcaacagca
     1381 tcctgcacat cctgccgttt caaatctaac actcgtaatt taccctttac ttcggcgaca
     1441 gattcgattc gcctgtaagc atcagaatga tgttcggcat atagcgcaag tccttccagt
     1501 ttgggcaaag catcagccag atgtaggcta tgtggatcta ccaggtccac aaccattttg
     1561 ccatcctgtt cggcaaagaa caggaaatcg ggacgaacaa tcttgtactg ttcagcttca
     1621 acataagcga ttcccagtga cgactgccct gtgtactgtg gattacgata ccagaaagca
     1681 aatccttcac gtttggtttc gatctcaaaa accttggttt cccaatggtt caacagagcc
     1741 ggatagttcc cgctttcgtc acacaacaaa tggtgtttcc agactggaag gtcagcttcc
     1801 ttttcacctt cacgcaccct ggtcatttca aacttgttcg ccggcctgac cagatccaca
     1861 tcctgcggtt cggtactcat ttcgacgatt tgacgatagg cctctttacg ctcatctttc
     1921 aacgacttga tttgcggtgt gtactcggcc agccacgcct tcgccagtct atcggcctcg
     1981 ccatcgaagt aatcctgcac ctccatcacc agccccatgc cagcaacggt aacacgagcc
     2041 tccatgattg cttccagata ttcctctgca tcctcttctc gatccgcaac cttatcggcc
     2101 agatattcga cataggtacg tgaaacatcc gggctgaaaa ttcgggctgc acgccggtag
     2161 gcgtcatcaa tcaccgttac gtccgcctcc tcccagaatt cgttgaaggt cattgctttg
     2221 ttcttgaggc tggcaaccac agccttacca tctacgacga gcactgcatt gcgtttgttt
     2281 ttgattttct cggcattttc tatctggaac ttgtccagaa cagcgtgcaa agcagcatgg
     2341 gctttcctga ctgccccatc aagaataccg tcatcaacca gttcctgtgc cagcgcggtc
     2401 agacgtctgg cgggtctggc tcctttcggt ggtcgggttt gcgaaggtag cgcttcaaat
     2461 gcttcccata cttcagctga agcagccggg ttaggtttca tctccacagg gttaaccagg
     2521 atgcgtccgc ttggaggaga ggtatcatcg cccttaaaca aggtattgac ggtgatg
//
LOCUS       DAOFQD010000262         2566 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.266, whole genome shotgun sequence.
ACCESSION   DAOFQD010000262 DAOFQD010000000
VERSION     DAOFQD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.266"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..365)
                     /locus_tag="QBF46_004785"
     CDS             complement(<1..365)
                     /locus_tag="QBF46_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676863.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCYL"
     gene            complement(627..2252)
                     /locus_tag="QBF46_004786"
                     /pseudo
     CDS             complement(627..2252)
                     /locus_tag="QBF46_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhiJ/YhiL family protein"
BASE COUNT          826 a          458 c          398 g          884 t
ORIGIN      
        1 tcaagatagc agccgtaatt tttactatta attgtcacaa taaactgata attattatcc
       61 ctacgtagaa tatgagcgcg catgcccgat agcatactat atttttctga atgattcgtt
      121 tgtatggtaa atgatatttt gtcatctcga atagattgtt ggaaacgttc tatttgccaa
      181 tcatcaattt tttccacaac ttcaccgagc attgtcctgg caatacttga tatatcatca
      241 cctataccat ttgcaatata cccaaacagt tctttagtga agttagtaac atgctcttgc
      301 accatatcat ttgttgcgat tgtcgttgta ctgtcgttag tgcctgccac agttccaatt
      361 ttcatctttt acctcatata attcaccgcg aggaatgcat agttaacatt taatcttatg
      421 acatacacct catgttcgat ggaaaaataa ttatattttg cacaaatcat tcagccttaa
      481 agcataatat gaaatttctt atctcatagc agagaaagtc cctaaagatt gtagaggcgt
      541 catcagaaca tgcctctaca atcgattaag aaaacctatg aaatactggc aggattaaaa
      601 agacatacca ccgtattcat ggagttttaa tgagattgaa aacaactaag ccctgaggaa
      661 taaattattt tattattttt atcacgtgct tccttcaggg aatttagccc cataatacca
      721 ttatcaaatt gttgattctg atctaccccg ggcccatgat gcccatattt ttgtctaatg
      781 tatttgctgg ggtcttcacc tttgagtgtc tccccacata tgaagatacg ttcacgtgct
      841 ggtctgaaat cagtagtgtt atcaaaataa ttagcatata caggtgtggg tttcaaagta
      901 ttgcttcgga ttagatggaa tagaggatca tccatgtgtt ctttatgttt tttattaaaa
      961 atattggcta attcctcagg atcagtagga tttttcatct gattaaatgg aacctgcaaa
     1021 gcaccgagac aataacattt tttttcttca tctcttatta ttccgtgatg actggttaaa
     1081 acttgccttt ctgagtcata attacaaaca tcgagcgttt taattaagtt atacgtatca
     1141 ccggcagaga gtttataatt ttctagtcat gcagtatagt taccattaaa attgatttca
     1201 tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt accctccata
     1261 tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta
     1321 tcgcctgtat gatccccaag gaagatacaa ggggttatcg catcgtcctt taaaaaaaca
     1381 tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta gagcaattta
     1441 ttatttttat gtatgtatgc agatgaagcc atgttttcgt gttttaataa ttcacataat
     1501 aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatgc ataaagtaca
     1561 gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc
     1621 acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata gctatcaaac
     1681 ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt aaaattaccg
     1741 ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt ataagttatg
     1801 tctgatttat aaattatgtc tgatttatct ccggagaaat taataaaata ataaccattc
     1861 ttacactcaa tcttaactgt aatgggagac gataatttta tcacatggtt attgtctggt
     1921 ataattgtaa agataaacgt gttagtagta ttatcggcga aactattata ttcttgaata
     1981 tctatagatg gctcactatt gtaaaaccat attttctcca tctgaagact atgttcattt
     2041 accctccagt gaccatgttg caggaaatag atgatattca ggcctaaatc gttatctatt
     2101 tgcgaaaagt cattcagcat attaacttca actgtgtttg atcgatattt tttatcaacc
     2161 ttgccatggt gttgatgact gttaacttta tctgaatgct tatcttgatg gtttgaagag
     2221 aagtgcttaa atatattgtg aaatactttc ataaataacc tgtaatattt ttacttgtta
     2281 gtaacaaaat aaataataca tgtctgaaag acatatttac gatgatacaa agcaataata
     2341 cttatatgtt cgatgatttt aagcaccgag actatccggt cttttactaa ataatggaaa
     2401 acaatccatc cctggaagat aattgtaaaa aaaacaatag aataagaatt aatcttagga
     2461 taaatattta tttatcatgg ccttttaacc ggtaaaaaat ggcactcggc acagtaaacc
     2521 ccgctatagt tatgctatgc atttgtcttt aatgatatct cgtcat
//
LOCUS       DAOFQD010000263         2557 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.268, whole genome shotgun sequence.
ACCESSION   DAOFQD010000263 DAOFQD010000000
VERSION     DAOFQD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.268"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..800)
                     /locus_tag="QBF46_004787"
     CDS             complement(<1..800)
                     /locus_tag="QBF46_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDQ6676864.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVG"
     gene            complement(826..1518)
                     /gene="rfaY"
                     /locus_tag="QBF46_004788"
     CDS             complement(826..1518)
                     /gene="rfaY"
                     /locus_tag="QBF46_004788"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDQ6676865.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            complement(1528..2523)
                     /locus_tag="QBF46_004789"
     CDS             complement(1528..2523)
                     /locus_tag="QBF46_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HDQ6676866.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDVNFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          815 a          493 c          422 g          827 t
ORIGIN      
        1 ccaacataat gaatcagctt agtatcatcg gtaatggtct ttttataatc ttcatcgctt
       61 ttatttttta actcataatc gatgccataa aagcaatcat aatcgccgct aataaaaata
      121 caatgaccaa aaaacagaat atttaaaata tcctgatcaa ggcatttata cttcgtgtgc
      181 tgcgggtcac ttgccagcat agcaatggct ttattcataa aatcattttt cgcccatgct
      241 tcaagctgca gatatactac accggcgttg aaataattcc ctggaagtcc ctctatagcc
      301 agacgcttcg ctggtttttc ctgcataaaa ggcacatctt taataacagc tgcaaactta
      361 tccccatcaa aattaatatc aagcaatcca gctaatgagc ctttacagat gacatcagca
      421 tcgatatata acagattttc aatagtttca ctcagatact ggaacgcaac taaacggaag
      481 aatgttgcat aagaccactg tttcaggctg gggaagatat caataaaggc attggagact
      541 ttataaattc ttacttcaac gttagcattt ttgctggtaa aagcattaac acgttgtaga
      601 taatcttcat caatgtagtc ggtaaagaga tgaaacttaa ccgcaaggtc cgggttatgc
      661 ataacaactg attgcataga tactgccgca ccaaataaaa agttacggtc aataccgtaa
      721 gcgatattta acacacgatc ggtgttagca atttccccag agacagcgac ttcagtaata
      781 ctctcagcta ataaatccat gttggttccg ttttgactgt gtggtttacg ctttgccttt
      841 taatttttta ataaatttgc gtagtttggt tctgtagatt actgaatagt agccataatc
      901 ttttatttcg ttggcaatac ccaggtgacg ttccatggcc agacgatcac gagctttacg
      961 ctctgccgta cagcttttac cggataggtc aattatcctg acaccatctt tactaacgat
     1021 aaaattaccc cggtgtgggt cgcccgatag catatttaag gcatgcaatt tttccattga
     1081 agccttaatt tctgctttaa cattttctgg aataatcggc atatcattga gctctacgcc
     1141 ctcgacatac tcgataagca taataaaaac actggcatag ttgaaaattt tacgctcagc
     1201 cagaaaataa aaatcattag gaaaagtaag acctgcactt ctgacacgat cggtttcaac
     1261 gatcaaattc tgatagtagt caccttttac aaaagatttt aaaaaacgtt cattcctttt
     1321 cgttttcggg gcaaagactt taaaaacata gcgaccatat tttgtatcaa tcaatgatac
     1381 tttggtatca tcaatattac gtaatacttt tactgtttta aaattatagg ccagtatttc
     1441 atcaaagatt tctttatatt tattatcatt atctttgtaa taaaatgaga agccgcgata
     1501 gcgtatactt gtaatcataa gagtaattta tttcttaagc ttgtacttaa ttaatgaagt
     1561 tatgcctttt atatactcac catgggcaaa cagatgctta tattgtttct gcatttctgc
     1621 aacagtccgc gcctctttaa gaggatattt cttccaggga gattgttcac gcgcgatatt
     1681 aaagtatgat gcagacggat atccagccca gctatgccat ggtttagtta tccctgtata
     1741 gtgaatcaac actgttttat cggtaatggt ttgctgatac tttcgatgac tacgatcata
     1801 aagttcgttt ttcagggtat aaatagtatc aaaatccttc gcaaggtaga tattattcat
     1861 attaaatgcg atattcaacg catcctgatc taaatattta agagagccat atttagtttc
     1921 ccctcgtaaa agtttgagta aatacggtgt caaatttgct tcatgccatt ttttaagatt
     1981 gacatagata acgcctgcat tgaaataacg tccattcatt tcgggaatgt tgaggcgttt
     2041 tgcacatgcc gcctgagtac tatcattgtc aggaatgacc gcagcaaact catctttaaa
     2101 tataatttct gtgagaggtt tcaggcttcc tttacaaaca acatcggcat ccagatacag
     2161 cagtgtggaa atactttcac tcagatattc aaaagacaat accctgaagt atgtcgcgta
     2221 cgaccaaaac tgtgacgtcg gcagatcagc aaagtatttg gggtcaatta aatatacaat
     2281 aattgaggta ttaaattgtt ttgctgtttc attaaatgac tttaaataat catcatccac
     2341 ataatcagtg aaaacatgaa agacaaaatt aacatctgaa ttattaatca aaacggaaga
     2401 aattgaaacg ccagcaccat acaaaaaatt cttatcgata ccgtaggaaa catttagctc
     2461 tggggcgttt tcttttttat tatctgctaa atacgaaaac cgttctttta taaattcatt
     2521 catttaactt ttctcaattt cattttatta tctttaa
//
LOCUS       DAOFQD010000264         2552 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.269, whole genome shotgun sequence.
ACCESSION   DAOFQD010000264 DAOFQD010000000
VERSION     DAOFQD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.269"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..701
                     /locus_tag="QBF46_004790"
     CDS             <1..701
                     /locus_tag="QBF46_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDQ6676867.1"
                     /translation="ERKYLTEDFQSVLNLKEESHLTNTPALVWISQPDVQKTKGNRAG
                     EHTPIWSNESECKAIIDILKDLDKNSNPTKKIKLAILSPYSNQVKLISKHIEIEKKKN
                     QFKTLLNNYIPPDDNDGYCLTVDSFQGGEADIVLISLVRNNGKSTIKSALGFLSDQRR
                     INVLLSRAKYKLIIVGSYELLKAWSNRISRENSKEYDFIPRLTDKLDLSLNERKLSLI
                     TSNFITNKGNKNEK"
     gene            691..2508
                     /locus_tag="QBF46_004791"
     CDS             691..2508
                     /locus_tag="QBF46_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676868.1"
                     /translation="MKNKNIYVKIPFHYGVHKFKIFKGHRWGALDHFLLQEISLQPYP
                     IEELSLKSNLPQRLIIEIILPFMKLGWVELVELNSKYNFRITSKGRSVANREELPYER
                     EPLESTRKFLIDPITAKCYRVNARNQNYQIYPTSRANELLKNKHSISTELKIKNPKHS
                     PFTSDILNCVEDTDEEVIGYEERANDRPYYQNRTFAIAQVDEADNITGVPSDISKELA
                     ADIIAAANMKRSEINTNIDSLSKNSKISTYNTESFENRFEEHYINETEFRIISGSDSH
                     RDHLIAMIDKSVSRIIIHSTFIHLKNFESIFQKLTDAAKRGVQVDILWGQEEPDDERS
                     IGSYNQFLEGLKSYREEIIKLGLTSLFTIHSDPTGSHAKVIVCDTMEFGYCSTIGSCN
                     WLASGFNRYECSVFVTNDTLTTEVLDILSIISKGKSRVSNNLSKSISAISYELKKACE
                     HLSSEDSANKNVRIKIITKNEHHDFVLDARDKATKSIFIASHRISNNAERPILTPLIT
                     SMTANSSLNINMYYSSLSGGINSQQLDDMSNSLRKNGITLEKKKDPISHAKILSWDND
                     NILITSLNWLSASAYGNPYDELGIFIERKDIFSLISPNY"
BASE COUNT          978 a          414 c          417 g          743 t
ORIGIN      
        1 aagaaaggaa gtatcttaca gaagattttc aatcagtact taacttaaaa gaggaatctc
       61 acctcacaaa tacaccagca ctggtatgga ttagtcaacc agatgtacaa aaaactaaag
      121 gtaacagagc aggagagcat acaccaatct ggtcaaacga aagtgaatgt aaggcaatca
      181 tagatattct caaagatttg gacaagaact ccaacccaac aaaaaaaata aagttggcaa
      241 ttctttctcc atactcaaat caagttaaat taatttcaaa gcatattgaa attgaaaaga
      301 aaaaaaatca attcaaaaca cttttaaata attacattcc accagatgat aatgatggtt
      361 attgccttac tgttgattcg ttccaaggtg gcgaagctga tattgtctta atttcattag
      421 tacgaaacaa tggtaaaagc acaattaaga gtgcattagg atttttatca gatcaacgcc
      481 gtataaatgt tttacttagt agagcaaagt acaaattgat catagtcggt tcatatgaac
      541 tcctcaaggc ttggtctaat cgtatatcca gagagaactc aaaagaatat gattttattc
      601 caagactaac tgataaactg gatttgtctt taaatgagag aaagctttca ttaattacat
      661 caaactttat aacaaacaaa ggaaataaaa atgaaaaata aaaacatata tgttaaaatt
      721 ccttttcatt atggagttca taaattcaaa atatttaaag gccatagatg gggagcttta
      781 gatcattttt tacttcaaga gattagcctc caaccctatc ctatagaaga gctatcatta
      841 aaatctaatc ttccacaacg gctcataatt gaaataatat tgccatttat gaaattgggt
      901 tgggttgaat tagttgaatt gaattctaaa tacaacttca ggattaccag caaaggaagg
      961 agtgttgcta atcgtgagga actcccttat gaaagagagc cactggaatc tactcggaag
     1021 tttttaatag atccaatcac agcaaaatgt tatcgagtaa atgcaagaaa tcaaaactat
     1081 cagatatatc ctacatcaag agcaaatgaa ttacttaaga ataaacactc gatatcaaca
     1141 gaactcaaaa ttaaaaaccc caaacattca ccgttcacat cagacattct aaattgtgtt
     1201 gaagatactg acgaagaggt tatagggtat gaggaaagag ctaatgatag accttattat
     1261 caaaatagaa cttttgctat agcacaagtt gatgaagcag ataacataac gggtgttcca
     1321 tctgatatct caaaagaatt agccgcagat attatcgctg cagctaatat gaagcgaagt
     1381 gaaataaata ctaatataga ctctcttagc aagaactcaa aaataagcac ttataatacc
     1441 gagtcttttg aaaatagatt tgaagaacac tatattaatg aaactgaatt ccgaataata
     1501 tcaggttcag acagccatcg agatcatctt atcgcgatga tcgataaatc tgttagtaga
     1561 atcattatcc attcaacttt tatacactta aaaaactttg aatcaatctt tcagaaatta
     1621 actgatgctg ctaaacgcgg agttcaagtt gatatcttat ggggccaaga agaacccgat
     1681 gatgagagaa gtatcggaag ttataaccaa tttcttgagg gccttaagtc ttatagagaa
     1741 gaaattataa agcttggatt aacctccctt tttacaatac attcagatcc aacaggttct
     1801 catgcaaagg ttatagtatg cgatacaatg gaattcggat attgttcaac catcggttca
     1861 tgtaactggt tagcaagtgg atttaatagg tatgaatgct ctgttttcgt aacaaatgat
     1921 accttgacga ctgaagtatt agatattttg agcataattt ctaaaggaaa atctagagta
     1981 tctaacaact taagcaaatc tatttcagcc atttcatacg aattgaaaaa agcttgtgag
     2041 catttatcat cagaagattc tgcgaacaaa aatgtaagaa ttaaaatcat cacaaaaaat
     2101 gagcatcatg attttgtttt agatgcaaga gacaaagcaa caaaatcaat atttattgcg
     2161 agtcatagaa ttagtaataa tgcagaaaga cctattttaa ctcccttgat aacatcaatg
     2221 acagccaata gcagtcttaa tattaacatg tattactcat ctcttagcgg aggtatcaat
     2281 tcacagcaac ttgatgacat gagtaactct ttaagaaaaa atggaattac tttagagaag
     2341 aagaaagatc ctatttctca tgcaaaaata ttatcatggg ataatgacaa cattttgatc
     2401 acgagtctta attggctttc agcctcagct tacggaaacc catatgatga gcttggcatc
     2461 tttatagaaa gaaaagatat attttcttta atttcgccta attactgaaa caagctatgg
     2521 gatatcttgt agaaagatat cctattgtac tt
//
LOCUS       DAOFQD010000265         2518 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.270, whole genome shotgun sequence.
ACCESSION   DAOFQD010000265 DAOFQD010000000
VERSION     DAOFQD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.270"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            64..1494
                     /locus_tag="QBF46_004792"
     CDS             64..1494
                     /locus_tag="QBF46_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDQ6676869.1"
                     /translation="MIRKSATGVIVALAVIWGGDTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGTPNLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHHELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1535..2467)
                     /locus_tag="QBF46_004793"
     CDS             complement(1535..2467)
                     /locus_tag="QBF46_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDQ6676870.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
BASE COUNT          785 a          522 c          557 g          654 t
ORIGIN      
        1 ctggacatta tttcagcata agtggatatt caaagttttg ctgttttatc agggaatatt
       61 tatatgatac gtaagtcagc tacaggtgtt attgttgcgt tagccgtaat ctggggtggt
      121 gacacatggt acacaggtac gcaaattcag cctggtatcg aaaagtttat taaagatttt
      181 aacgatgcga aaaagaaagg tgaacatgcc tacgatatga cgttaagtta taaaaacttt
      241 gacaaaggct tttttaattc ccgttttcaa atgcaaatga cattcgataa cggtacaccc
      301 aatctcaata tcaagccagg ccagaaagtt gtatttgatg tggatgttga gcacggtccg
      361 ttgcccatca caatgttaat gcatggtaat gtcatcccag cactggcagc ggcaaaagtg
      421 aacttagtga ataatgaact gacacaaccg ctatttatcg ccgcgaaaaa taaatcgccc
      481 gtggaagcga cattgcgatt cgcgtttggt ggctcattct ctacgacatt agatgttgcc
      541 cctgcagagt atggaaagtt ttcttttggt gagggccagt ttacttttaa tggtgatggt
      601 agttcattgt ctaacctgga tattgaaggc aaagtcgaag atattgttct gcaattatca
      661 ccaatgaaca aagtaacggc aaaaagtttt accattgatt ctctggcgcg attagaagaa
      721 aagaaatttc cggttggtga aagcgagtcg aaatttaatc agattaacat tatcaatcac
      781 ggggaagacg ttgcccaaat cgatgctttc gttgcaaaaa ccaggctgga tcgcgttaaa
      841 gacaaagatt atatcaatgt caatctcacc tacgaacttg ataagttaac aaaagggaat
      901 cagcaactcg gtagtggtga gtggtcattg attgctgaat ctattgatcc ctcagcagtg
      961 cgccaattta tcatccagta taacattgcg atgcagaagc agcttgctgc acaccatgag
     1021 ttagcaaacg atgaagttgc tctgcaagaa gtgaatgctg cattgttcaa agagtattta
     1081 ccgttattac aaaaaagtga gccgaccatt aaacaaccgg taagatggaa gaacgcactc
     1141 ggcgaactaa atgccaatct ggatatcagt attgccgacc cagccaaatc ttcatcatcc
     1201 acaaacaaag atatcaaatc gctcaatttt gatgtgaagt taccgcttaa tgtcgtcaca
     1261 gaaaccgcaa aacagcttaa tttatctgaa ggaatggatg cggaaaaagc gcaaaagcag
     1321 gctgataaac aaatcagcgg gatgatgaca ttaggtcaga tgtttcagtt aatcacgatt
     1381 gacaacaata ccgcctcgct gcagctgcgt tatacaccgg gtaaagttgt ttttaacgga
     1441 caggagatga gcgaagaaga atttatgtct cgtgccggac gttttgttca ttaaaatact
     1501 cactcgcctc tgctcattca gaggcgagta aaacttactt attctgacgc tgtgatgaca
     1561 tgagagacgt cagaagccgg tctttttcct gccacaatga gttcagccat tgctgaaaat
     1621 ggcgcttaaa gcttttgtca ttgatgtagt cgccatgtaa ctcatcggca ataggctgta
     1681 aatccacatg tacgacaatc cgcgtcagtt taccgcttaa catatcgaag aacggctgac
     1741 ggttattgtc cggataacac agcgtaacat taagcagttt atcgaattgt ttacccagta
     1801 catttagcgc catcgcaatg cctgccgcct ttggtggcaa caagttttga aaagtggagt
     1861 gggtttgctg atgtttttct tgcgtgaaac gggagccttc aacgaaattg acaatggtgg
     1921 tgggatgcag gcgaaacttt tcacaagaac gccgagtggt ttcaacatct ttaccacgtc
     1981 gctccggatg acgtaataaa taagcgcggg aataacgctt cataaacggc atatccagcg
     2041 accagcacgc caggccaaga aatggcaccc aggccagctg ttgcttgagg aaatatttat
     2101 tcatcggaat gtgcttacga aacagcacgc acagtacgac aatatctgcc caactacggt
     2161 ggttacaaat aagcagatac cagttcttct tacttaaccc ttccagcccg tgaacttccc
     2221 attgcaggtg tgggttcaga tgcagtaata ccgccagacc ttcacaccag caatacatca
     2281 taaaatcaca aaaacgcgaa acctttcgcc aaatgactgg cacaggtagc aatagtttta
     2341 caatcccggc aatgatgatc gggacggaac aaaaaatagt gaccaaaatg gtcaatacga
     2401 tactcagcaa aagggttatc gcagcgagta ttctcgtcat aatgaattta ttcaaaaggt
     2461 tagccataga cagtgcgcta aagaagcgca aggcgcagat tttagcagaa aacgaccc
//
LOCUS       DAOFQD010000266         2493 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.271, whole genome shotgun sequence.
ACCESSION   DAOFQD010000266 DAOFQD010000000
VERSION     DAOFQD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.271"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..2223
                     /locus_tag="QBF46_004794"
     CDS             <1..2223
                     /locus_tag="QBF46_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6676871.1"
                     /translation="LADASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAG
                     LLNYSFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDS
                     NKWQHINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFA
                     PVIHGIARGTAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSIQ
                     TLYVPYSSVPVLQRAGYTRYALAMGEYRSGNNLQSSPKFIQGSLMHGLEGNWTPYGGM
                     QIAEDYQAFNLGIGKDLGLFGAFSFDITQANTTLADGTHHSGQSVKSVYSKSFYQTGT
                     NIQVAGYRYSTQGFYNLSDSAYSRMSGYTVKPPTGDTNEQTQFIDYFNLFYSKRGQEQ
                     ISISQQLGNYGTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQN
                     DRDHLLAFTLNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNN
                     LNYSVQVGNTHGGNTSSGTSGYSSLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIAH
                     ADGITFGQPLGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALNA
                     NSLADNVELDETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGEN
                     KNGSIVAENGQVYLTGLPQSGKLQVSWGKDKNSNCIVEYKLPEVSPGTLLNQQTAICR
                     "
     gene            2237..>2493
                     /locus_tag="QBF46_004795"
     CDS             2237..>2493
                     /locus_tag="QBF46_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6676872.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVR"
BASE COUNT          705 a          531 c          572 g          685 t
ORIGIN      
        1 ttggccgacg catcaactga gtttgatgtt ggtcagcaac atttgtcact ctccgttccg
       61 caaatttatg ttggcagaat ggctcgcggc tatgtttctc ctgatctgtg ggaagaagga
      121 ataaatgctg ggcttctaaa ctatagtttt aatggtaatt ctattaataa tcgtagtaac
      181 cataatgcag gaaaatccaa ctatgcatat ttgaatttac agagtggcat caacattggt
      241 agttggcgac tgcgcgataa ctcaacgtgg agttataaca gtgggagtag caattcatct
      301 gacagcaata aatggcagca tatcaatacg tcggctgaac gtgacattat tcccttacgc
      361 tcacggttaa cggtaggtga tagttatacc gatggcgata tttttgatag tgtgaacttt
      421 cgtggcctca aaataaattc aacagaagcg atgttgcccg atagccaaca tggtttcgct
      481 ccggtgattc atggtattgc ccgtggaacc gcacaagtga gtgtaaaaca gaacggatac
      541 gatgtttatc agactactgt tccacccggc ccttttacta ttgatgatat caactctgcg
      601 gccaatggtg gtgatctgca agtaaccata aaagaggcag acggcagtat tcagacatta
      661 tatgttcctt attcgtctgt tccggttctc caacgtgctg gatatacgcg ttatgcgctt
      721 gccatggggg aatatcgtag tggaaataac ctgcaaagct ctcccaagtt catacaaggt
      781 agcttgatgc atggtctgga aggaaactgg acaccttatg gcggaatgca aattgcagaa
      841 gattatcagg ccttcaacct tggtattggg aaagatttag gactttttgg tgccttttct
      901 ttcgatatca cgcaggccaa tacgacactt gcagatggca cccatcacag tgggcaatcg
      961 gttaaatccg tctacagcaa atccttctac cagacaggaa ccaatatcca ggtcgcagga
     1021 tatcgctatt ctacgcaagg tttttataac ttatccgaca gtgcctacag tcgaatgagt
     1081 ggttacaccg tcaagcctcc taccggagac accaatgagc agacacaatt tattgattat
     1141 tttaatctgt tctacagtaa gcgtggtcag gaacaaataa gcatctctca gcagcttgga
     1201 aattacggta cgacattttt cagtgccagt cgccaaagtt actggaacac gtcacgcagc
     1261 gaccagcaaa tatcatttgg attaaatgtg ccgtttggtg atattacgac ttcgctgaat
     1321 tacagctatt ccaataatat atggcaaaac gatcgggatc atttactcgc ttttacgctt
     1381 aatgttccct tcagtcattg gatgcgtaca gacagtcagt cggcatttcg taattcaaac
     1441 gccagttaca gtatgtcaaa cgatttgaaa ggcggcatga ccaatctatc gggggtttat
     1501 ggcactctgc tgccggataa taacctgaat tatagcgttc aggtcggtaa cacccacgga
     1561 ggtaatacat cgtctggcac cagtggttac agttctctta attatcgtgg agcttatggt
     1621 aatactaatg tcggttacag tcggagtggt gacagcagcc agatttatta cggaatgagt
     1681 ggtgggatta ttgctcatgc tgatggcatc acctttggac agccgctggg cgacacaatg
     1741 gttctggtta aggctcctgg tgctgataat gtcaaaatag agaaccagac cggaattcat
     1801 accgactggc gtggctatgc catattacca tttgcgacag aatatagaga aaaccgtgtt
     1861 gctcttaacg cgaattccct tgcagataat gttgaactgg atgaaaccgt ggtcactgtc
     1921 atcccaactc acggtgctat tgccagagca acatttaatg cacaaatcgg cgggaaagta
     1981 ttaatgacgt tgaagtacgg taataagagc gttccattcg gtgcaattgt cacacacgga
     2041 gagaataaaa atggcagcat tgtcgcggaa aacggtcagg tttatctgac tggacttcca
     2101 cagtcaggga aattacaggt ttcatggggt aaagataaaa actcaaactg tattgtcgag
     2161 tacaagcttc ctgaagtttc tcctggtacc ttactgaacc agcagacagc aatctgtcgc
     2221 taaggaacaa tagattatga aatacaataa cattattttc ctcggtttat gtctggggtt
     2281 aaccacctat tctgctttat ccgcagatag cgttattaaa attagcgggc gcgtcctcga
     2341 ttatggctgc acagtctcat cggattcgct taattttacc gtagatctcc aaaaaaacag
     2401 tgccagacaa tttccaacga ccggtagcac aagtccagcc gtcccttttc agattacgtt
     2461 aagtgaatgc agcaaaggga caacgggggt tcg
//
LOCUS       DAOFQD010000267         2490 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.272, whole genome shotgun sequence.
ACCESSION   DAOFQD010000267 DAOFQD010000000
VERSION     DAOFQD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.272"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            2..517
                     /locus_tag="QBF46_004796"
     CDS             2..517
                     /locus_tag="QBF46_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cymJ protein"
                     /protein_id="HDQ6676873.1"
                     /translation="MMFQNDREFIYNVSGFDNLHQKAKEMLSSAEYEVYLNTDFDLHF
                     LKNEICEALNRHVRVIVFSFNKLITPDPRVELYYRFNHEEEYYPSHRFMLVVDMKTVM
                     VFSQRAESLGLFSNNRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQ
                     DEKHLSDSKNR"
     gene            605..2473
                     /locus_tag="QBF46_004797"
     CDS             605..2473
                     /locus_tag="QBF46_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDQ6676874.1"
                     /translation="MIFFNDIKPTGWLKNKIEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPLTVSAEDSVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNNEWHEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEHGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPVGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPIHHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTYNNVAVKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTTNDTQNSLIILDRTWQYHDVIDI
                     KIIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
BASE COUNT          787 a          444 c          460 g          799 t
ORIGIN      
        1 gatgatgttt cagaatgacc gtgaattcat ttataatgtc tctggttttg ataatttaca
       61 tcaaaaagct aaagagatgc tctcttctgc agagtatgaa gtttacctca acacggattt
      121 tgatttacat tttttgaaaa atgaaatttg tgaggctcta aatcgacatg ttcgagttat
      181 tgttttttca tttaataaac ttattactcc tgacccacgt gtagaactgt attaccgttt
      241 taaccatgaa gaagaatatt acccttctca tcgtttcatg cttgttgttg atatgaagac
      301 agtgatggtt ttttcacaac gagctgaatc actggggtta ttctctaaca atcgcttgct
      361 tattaatata attgccgaac atatccatag tgatatatat ctaaccaagt atcagcaaca
      421 atatcctgat gccaatgcat taataaatac tttgcatgaa tctggcaatg atatggtgca
      481 ggatgaaaaa catttgagtg atagcaaaaa ccgttaatat tttcctttca ttgtgatcgt
      541 ctgcaggcga tcacaatcta tttgtctttt ataacgtcta aattttttat gcaaaggtat
      601 atatatgatt ttttttaacg atattaagcc cactggttgg ttaaaaaata agatagaatg
      661 gtatatgaac ggtcatatcg gagaactgga taaattagtt ccccatctta ttacggagca
      721 taaaatatat ggaagagatc gtataacccc actcactgtt tctgctgaag atagcgttat
      781 aaaaaaacag aataatcctg atgataacat gatgcaatat tattggtggg atagtgaaac
      841 tcaatcgaat tggaaggatg gatattgccg cagtgcctac ttgttagata ataacgaatg
      901 gcacgaaaaa gcgagcgatt tatgtttgga acttatcgat acgcaagatg atgatggcta
      961 cattggaatt tatgattcat cattaaaatt taactgcaaa gcagaaaacg gagagttctg
     1021 ggcacaagct actgcattgc gttttgtgct tggatgttat gaatccaccc gtaatgagag
     1081 tttattaacc tctcttaccc aagccgcgca atgcattatg gctggctacc ctaaagaaac
     1141 gtcacatcct ttcgttgtgg aacatggttt tgctggacat agccacggat taacaataac
     1201 agatgttttc taccgccttt atgaaataac caataacatt gaatatctct catattgctt
     1261 gtggctgtat gaagattatt ccgcaggatg cgtgtcggag caagatctgc aatataaaaa
     1321 tgtcatagac cctggttacc ttcttaaagg tcatggcgta catacatatg agcatattag
     1381 ggcattagcc atcgcagcga gccaacggcc gcagtatcaa actgcattag atattttttt
     1441 atcccagttg aaatattatc ttacaccatc tggcgctccc gttggtgatg agtggattac
     1501 aggacgcaca gctaatgcca cttatacagg ctatgaatat tgttcagttc atgagctatt
     1561 tagcacttat gttatgttaa taaaacttac cactaacatt ggtcttgccg atgatgctga
     1621 gtggctattc tataatgcca gttttggcat gcagcatccc atacatcacg cgataatgta
     1681 ctgtaagact gataattgtt atgaagcaaa tgaaaggaaa catccggaca gtaatcagtt
     1741 taataccaga tataaatact cccctactca tgaggaggct gctgtctgct gcgtaccgaa
     1801 tacagcaaga accataccag cttttgtcga aaatatgttt caagaaaata acaatggttt
     1861 tactgcatta ttttatggtc cgtgtgagtt ttatggcaca tataataatg ttgcagttaa
     1921 aattacacaa ttgactgaat accctcatga tttaagcgta aaatgcctca ttgagcctga
     1981 gtcatctgtt cgttttgctc tcagtttccg ttaccccggc tgggcaaaaa tgatgattat
     2041 taatggtgtt acttttacta caaatgatac ccaaaactca cttattattt tagatagaac
     2101 atggcaatat catgatgtga ttgacataaa aattatagct gatatacagt ttaatactga
     2161 cttatgtggt gacacttata tttccagagg tcccgtttta tatgcaattg agcttgagag
     2221 cgatatttta atcaaaaaaa acctgctgaa cgggaaatat tttgactcag gatacatacc
     2281 ctgtagcaga gctgatgaat ctatccagtt ctcttatgct gatatggagt ctttttcata
     2341 ttcaacctca ccagagggca aacaatacat tgaaggctgt ttttatttaa acaagactaa
     2401 aatagtcaga aaacttatac cattcggtca aaccattctt cgcaaagtaa ctttctccaa
     2461 tatagagagt taatattttc gagtattata
//
LOCUS       DAOFQD010000268         2440 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.273, whole genome shotgun sequence.
ACCESSION   DAOFQD010000268 DAOFQD010000000
VERSION     DAOFQD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.273"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..2088
                     /locus_tag="QBF46_004798"
     CDS             <1..2088
                     /locus_tag="QBF46_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDQ6676875.1"
                     /translation="ITLWHYDASDRITHRTVNGEPAEQWRYNDHGWLTEISHLSEGHR
                     VAVHYGYNRKGRLTGERQTVHHPETGELLWQHETQHTYNEQGLANRFQADSLPPVEWL
                     TYGSGYLAGMKLGDTPLVEYTRDRLHRETVRSFGNNAYELTSTYTPAGRLQSQHLNSL
                     VYDRDYGWNDNGDLVRISGPWQSREYGYSATGRLESVRTLASDLDIRIPYASDPAGNR
                     LPDPELHPDSTLTAWPDNRITEDAHYLYRYDRYGRLTEKTDRIPTGVIRTDDERTHHY
                     HYDSQHRLVFYTRIQHEEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVT
                     WYGWDGDRLTTVQTDTKRIQTVYQPRSFTPLIRIETENGELQKAQRRSLAEKLQQEGS
                     EDGHGVVFPAELVRMLDRLEGEIRADRVSSESRQWLAQCGLTVERLAAQIEPVYLPER
                     KIHLYHCDHRGLPLALISTEGATEWRGEYDEWGNQLNEENPHHLFQPYRLPGQQYDDE
                     SGLCYNRYRYYEPLQGRYITQDPIGLNGGWNLYKYPLNPINYADPLGLAVDINHFPVN
                     EDIRNYAEKVWNNPNIITIGAHGDPQSVYDENYNKIDVKTLANEVRNHPKFKPGMSVR
                     LLSCNTGKGNNSYAQQLADELGVRVYAPDQYDWYYSNGKVEPWGGFYDANNQLQMDTN
                     QPGHEFTFKRRKP"
     gene            2145..>2440
                     /locus_tag="QBF46_004799"
     CDS             2145..>2440
                     /locus_tag="QBF46_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDQ6676876.1"
                     /translation="MSNDSEKIISTYSLNFLSPDDVRKEELKASQGDSDAAFKLYKYY
                     LFCEPKSYRKQHEWLIISANNGNAIAQYNLSRELESEGKVDESIQWLKKSAEHG"
BASE COUNT          690 a          574 c          695 g          481 t
ORIGIN      
        1 atcaccctct ggcactacga tgcatcggac cgtatcacac accgcacggt gaacggcgaa
       61 ccggcagagc agtggcggta taacgaccac ggctggctga cagaaatcag ccatctcagc
      121 gaaggccacc gggtggcggt gcattacggt tataaccgta agggacgtct cacaggcgag
      181 cgccagacgg tacatcaccc ggagacgggg gaactgctct ggcagcatga gacgcaacac
      241 acttacaacg agcaggggct ggcaaaccgc tttcaggcag acagcctgcc accggtggag
      301 tggctaacgt atggcagcgg ttacctggcg ggaatgaaac tgggcgacac gccgctggtg
      361 gagtacacac gggatagact gcaccgggaa acggtgcgca gcttcggcaa taatgcatat
      421 gaactgacca gcacttacac ccccgcaggc cggttacaga gtcagcacct gaacagcctg
      481 gtgtatgacc gtgactatgg ctggaatgac aatggcgacc tggtgcgcat cagcggcccg
      541 tggcagtcgc gggaatacgg ctacagtgcc acgggcaggc tggagagtgt gcgcaccctt
      601 gcatcagacc tggatatccg catcccgtat gcctccgacc cggcgggaaa ccgcctgccg
      661 gacccggagc tgcacccgga cagtacactc acggcgtggc cggataaccg aatcaccgaa
      721 gacgcgcact acctgtaccg ctatgacagg tatggcaggc tgacggagaa gacggaccgc
      781 atcccgacgg gtgtgatacg gacggacgac gagcggaccc accactacca ctacgacagc
      841 cagcaccggc tggtgtttta tacgcggata cagcatgagg agccactggt cgagagccgc
      901 tatctctacg acccgctggg ccgccggacg gggaaacggg tgtggcgtcg cgagcgtgac
      961 ctgacgggat ggatgtcgct gtcgcgtaaa ccggaggtga cgtggtacgg ctgggacggc
     1021 gaccggctga cgacggtaca gaccgatacc aagcgtatcc agacggtata ccagccgagg
     1081 agcttcacgc cgctcatccg tatcgaaaca gagaatggtg agctgcagaa agcgcagcgc
     1141 cgcagcctgg cggagaagtt gcagcaggaa ggaagcgagg acggccacgg tgtggtgttc
     1201 ccggcagaac ttgtgaggat gctggacagg ctggaggggg aaatccgggc agaccgcgtg
     1261 agcagtgaaa gccgccagtg gctggcacag tgcgggctga cggtggagcg gctggcggca
     1321 cagatagagc cggtttattt acctgagcga aaaattcatc tttatcactg tgaccaccgg
     1381 gggctgccgc tggcgctcat cagtaccgaa ggagcaacgg aatggcgtgg ggagtatgac
     1441 gagtggggga atcagcttaa tgaggagaac ccgcatcatc tgttccagcc ataccgcctg
     1501 ccggggcagc agtacgatga tgaatcgggg ctgtgctata atcgttatcg ttactacgag
     1561 ccgttacagg ggaggtatat tacacaggac ccgattgggt taaatggggg gtggaattta
     1621 tataaatatc cgctgaatcc aataaattat gcagatcctc ttggcttagc cgtagatata
     1681 aaccacttcc cagtcaacga agacatacga aattatgcag agaaagtttg gaataatcct
     1741 aatatcatta caattggagc acatggtgat cctcaatcag tatatgatga aaattataat
     1801 aagatagatg tcaagacatt agctaatgag gttcgaaatc atcctaaatt taaacctgga
     1861 atgtcagtaa gactgttatc ctgtaataca ggaaaaggaa ataactctta tgcacagcaa
     1921 ttggcagatg aactaggtgt gagagtctat gctcctgacc aatatgactg gtactattca
     1981 aatggtaagg tagagccttg gggaggattc tatgatgcaa acaatcaatt gcaaatggac
     2041 acgaatcagc ctggacatga gtttacattc aagcgaagga aaccataggt atgaggtatt
     2101 ttctctttat atttataaca atccccctta tctctggagg tcatatgagt aatgatagcg
     2161 aaaagataat atcaacatat tcgctcaatt ttttatcacc agatgatgtg aggaaagaag
     2221 aacttaaggc atcacaaggt gatagtgatg ctgcatttaa attatacaaa tattaccttt
     2281 tttgcgagcc aaaatcatac agaaaacagc acgaatggtt aataatatcg gcaaataatg
     2341 gaaatgctat agctcaatat aacttatcaa gagaacttga gtcagaagga aaagtagatg
     2401 aatcaataca atggctgaaa aaatcagcgg aacatggtaa
//
LOCUS       DAOFQD010000269         2439 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.274, whole genome shotgun sequence.
ACCESSION   DAOFQD010000269 DAOFQD010000000
VERSION     DAOFQD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.274"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..767)
                     /gene="lacI"
                     /locus_tag="QBF46_004800"
     CDS             complement(<1..767)
                     /gene="lacI"
                     /locus_tag="QBF46_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDQ6676877.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNKASHVSAKTREKVEAAMAQ
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     SVEACKAAVHNLLAQRVSGLIINYPLDDEDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLIALGHQQIALLAGPLSSVSARLRLADWHKYLTRNQIHPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRA"
     gene            1120..1518
                     /locus_tag="QBF46_004801"
     CDS             1120..1518
                     /locus_tag="QBF46_004801"
                     /inference="COORDINATES: protein motif:HMM:NF019845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDQ6676878.1"
                     /translation="MKAVGFWQPGESTSVIEDLSLPHPSMPKGRDLLIRVRAVAVNPR
                     DLKSRRNISPSAGNPVVMGYDASGVVEATGPDVTLFRPGDHVFYAGVLDRQGANAEFQ
                     LVDERITGIKPAALSFEAAASVPLTGLTAW"
     gene            1519..2130
                     /locus_tag="QBF46_004802"
     CDS             1519..2130
                     /locus_tag="QBF46_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDQ6676879.1"
                     /translation="MLFDRLQLSVNGDADQTLLMLGGAGGVPCMAIQLARTLSRVNII
                     GTASRQESVRAVRGFGAHYVLDHSCALAPQVASLAGLPPITRIFSTYTTPSAWENMAE
                     IIAPQGRIGLIDDPEPLDLRLIKFKSVSVHWEAMFTRPMHATADMSKQHDILNKLSCM
                     LDSGKLKPPLLAEYGRLNAKALRRAHEDMENKNVNGKIVFSGF"
BASE COUNT          569 a          635 c          636 g          599 t
ORIGIN      
        1 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
       61 ccctcattca gcatttgcat agtttgttga aaaccggaca tggcactcca gtcgccttcc
      121 cgttccgcta tcggatgaat ttgattgcgt gtgagatatt tatgccagtc cgccagacgc
      181 agacgcgccg agacagaact taatggaccg gctaacaacg cgatttgctg gtgacccaat
      241 gcgatcagat gctccacgcc cagtcgcgta ccgtcttcat gggaaaaaat aatactgttg
      301 atgggagtct ggtcagagac atcaagaaat agggccggaa cattagcgca ggcagcttcc
      361 acagcaatgg catcctcgtc atccagcgga tagttaatga tcagaccact gacgcgttgc
      421 gcgagaaggt tatgtaccgc cgctttacag gcttcgacgc tgcttcgttc taccatcgac
      481 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
      541 ggcgcgtgca gggccagact ggaggtagca acgccaatca gcaacgactg tttgcccgcc
      601 agttgttgtg ccacgcggtt gggaatgtag ttcagctgcg ccatcgccgc ttccaccttt
      661 tcccgcgttt tcgcagagac gtggctggcc ttgttcacca cgcgggaaac ggtctgataa
      721 gagacaccgg catactctgc gacatcgtat agcgttactg gtttcacatt taccaccctg
      781 aattgactct cttcgggtgc tatcatgcca taccacgaaa tgttttgcgc cattcggagg
      841 gggcacgccg aagtgagatt caaaatgccg acgtcagttc acaggtgggt aatgatacca
      901 acgtgttgat ttcgtatttt cactgacacc aggatcatcc tgatgttaca aggattgaat
      961 gactacagat taaaatagtc atcaacaggt tgacagcatt acccagcgcc cgctttccgc
     1021 gtatttttcg atttgcactg ccgggggcac tggcgggtat cgcgttccgc acacttttac
     1081 atttatttat ctggtgactg acctttttaa ggagacagaa tgaaagccgt tggtttctgg
     1141 caacccgggg aaagtacatc cgttattgaa gatttgtcgc ttccccaccc atccatgccc
     1201 aaggggcgtg atcttttgat cagagtcagg gcggtcgcgg ttaacccacg ggacctgaaa
     1261 agccggcgaa acatcagtcc ctctgcaggc aatccggtag ttatgggata cgacgcttcc
     1321 ggtgttgttg aagcaaccgg accagatgtg acgctgttca ggccaggtga ccacgttttc
     1381 tatgccggtg tgctcgatcg ccagggagcc aacgcagagt ttcagctggt tgacgaacgc
     1441 attacgggga taaagccggc agcactaagt tttgaagctg cagcgtcggt tcctctgacc
     1501 ggtctgacag cctggtaaat gttatttgat cgtctgcagt tgtcagtgaa cggagatgct
     1561 gatcagaccc tgttgatgct tggcggtgcg ggcggagtcc cttgtatggc tatacagctt
     1621 gcccgcactc tgagtcgggt gaacatcatc gggactgcct cccggcagga gagtgtcaga
     1681 gcggttcgcg ggtttggtgc gcattatgtc ctggatcatt catgtgcgct tgcgccacag
     1741 gttgccagcc ttgctggact ccctcccatt acccggattt tctcaacgta caccacgcca
     1801 tctgcctggg aaaatatggc cgaaattatt gccccacaag gccgtatcgg actcatcgac
     1861 gaccctgaac cactggatct gcgtttgatc aaatttaaga gcgtttctgt tcactgggag
     1921 gcaatgttta cccgccccat gcatgctact gcggacatgt ctaaacagca tgatattctt
     1981 aacaaactct catgcatgct ggattccggt aagctcaaac ctccgttgct ggcagagtat
     2041 ggcaggctga atgctaaagc acttcgccgg gctcatgagg acatggaaaa taaaaatgtg
     2101 aacgggaaaa ttgttttttc agggttctga tgaacaggcc tgtcagagta aaaaaagatt
     2161 cgggttcagc acaatgctga attctgtctg gctatagagt aagaatcgta ccggaattga
     2221 gcatttaccc tcagtacctg cggatgaaaa agcagtctgc cctgacatac tgaacaatcc
     2281 cttctcataa gatgcctctc ttttgctgtt taaagagctg agcagtccgg gcggagactg
     2341 acctcgttcc gcttttcaag cgaccccctt gttgaaagtg cgacaaaaga gatagggcga
     2401 atgactatac ttcgctcata gctgcccgtc atgccagct
//
LOCUS       DAOFQD010000270         2432 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.275, whole genome shotgun sequence.
ACCESSION   DAOFQD010000270 DAOFQD010000000
VERSION     DAOFQD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.275"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..678
                     /gene="agaC"
                     /locus_tag="QBF46_004803"
     CDS             <1..678
                     /gene="agaC"
                     /locus_tag="QBF46_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDQ6676880.1"
                     /translation="ILGDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIA
                     WSTGVDAKTAIGLGLPFSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTT
                     MLIVASAYAVIAFLCTYLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMF
                     KAQYIPYLIAGFLFVCYIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEE
                     EDYSNGI"
     gene            668..1459
                     /gene="agaD"
                     /locus_tag="QBF46_004804"
     CDS             668..1459
                     /gene="agaD"
                     /locus_tag="QBF46_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDQ6676881.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1460..2214
                     /locus_tag="QBF46_004805"
                     /pseudo
     CDS             1460..2214
                     /locus_tag="QBF46_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT          582 a          581 c          615 g          654 t
ORIGIN      
        1 attctcggtg atattcagac tggcttaatt accggtggtc tgacagagtt ggctttcgcc
       61 ggattaaccc ctgcaggtgg tgttcagccg cccaacccga ttatggcggg tctgatgacc
      121 accgtcattg catggtctac gggcgttgat gccaaaacag caattggtct tggcctgccg
      181 tttagtttgt taatgcagta cgtcattctg ttcttctatt ccgctttctc attatttatg
      241 accaaagccg ataaatgcgc gaaagaggcg gatacggcag cgttttcccg gcttaactgg
      301 acaacgatgc tcatcgtcgc ttcagcgtat gcggtgattg ctttcctctg tacttacctg
      361 gcacaggggg cgatgcaggc gctggtgaaa gcgatgcccg cctggctgac ccacggcttt
      421 gaagtggcag gcggtattct gcctgccgtt ggttttggct tgctgctgcg cgtaatgttc
      481 aaagcgcaat atatccctta cctgatcgcc ggtttcctgt ttgtttgcta catccaggtc
      541 agcaacctgt tgccggttgc cgtactgggc gcaggctttg cggtgtatga gtttttcaat
      601 gcgaaatccc ggcagcaagc gcaaccgcag cccgttgcca gtaaaaatga agaagaggac
      661 tacagcaatg ggatctgaaa tcagtaaaaa agatatcacc cgtctgggct ttcgttcgtc
      721 gctgctgcaa gcgagcttta actacgaaag gatgcaggcg ggcggtttta cttgggcgat
      781 gttgccgatc ctgaaaaaga tttataagga cgacaaaccg ggcttaagcg cggcgatgaa
      841 agataacctc gaatttatta atacccaccc gaatctggtc ggattcctga tggggttatt
      901 aatttcgatg gaagaaaaag gagaaaaccg cgacaccatt aaaggcctca aagtggcact
      961 gtttggccca atcgccggga ttggcgatgc gattttctgg tttactttgt tgccgattat
     1021 ggcgggaatt tgctcatcat ttgccagcca gggaaacctg ctggggccga ttctattttt
     1081 cgccgtttac ctgcttatct ttttcctgcg cgtcggctgg acccacgtcg gttattcagt
     1141 cggcgtgaag gcgatcgata aagtgcgaga gaactcgcag atgattgccc gttcggcaac
     1201 catcctcggg atcacggtaa tcggcgggct gatcgcttcg tatgtgcata ttaacgtggt
     1261 gacatcgttt gccatcgaca gtacccacag cgtcgcgctg cagcaggatt tcttcgataa
     1321 agtcttcccg aacattttac cgatggccta caccctgctg atgtattact tcctgcgggt
     1381 gaaaaaagcg catccggtgc tgttaatcgg cgtgactttt gtgctctcta ttgtttgttc
     1441 cgcattcggc attttgtaaa tggaacgagg cactgcgtct ggcggtgcct ctttacttaa
     1501 ggaatttcat cctgtgcaaa cccttcagca agttgaaaac tatacggcgt taagtgaacg
     1561 tgccagcgaa tatttattgg ccgtgatccg tagcaaaccg gatgccgtga tttgcctggc
     1621 gaccggagcc acgccattac tgacgtatca ttatctggta gaaaaaatcc accagcagca
     1681 ggttgatgtc agccagctca ccttcgtgaa gctcgacgaa tgggtggatc tgccattaac
     1741 gatgcctggt acctgcgaaa ctttcctgca acagcatatc gtgcagccgc tggggctacg
     1801 tgaagaccag ctcatcagct ttcgctccga agagataaat gagacagagt gcgaacgggt
     1861 aacgaacctg attgcgcgca aaggcggtct ggatttatgc gttctcggat tggggaaaaa
     1921 cggtcatctt gggctgaacg aaccgggaga aagcctgcaa ccggcctgcc atatcagtca
     1981 acttgatgcc agaacacagc aacatgagat gttaaaaacc gcgggtcgcc ccgtgactcg
     2041 tgggatcacc ttaggcctga aggatattct caatgcccgc gaagttttgt tactggtgac
     2101 tggcgaagga aagcaggatg cgacagatcg ttttctcacg gctaagtctc taccgctatc
     2161 ccggcttcat ttttatggct gcacagtaac tttatttgtt taattaacac ctaaccagat
     2221 gaaaaatttg ctcaaaatgt ttaaattaac ttatgtaaca gtcacgcatt atattaaata
     2281 acatttgact gggttgaaca taacgccgat agcaaaagga gttatgttca acaatttaat
     2341 tttagatata catataactt gttctattaa taaatcacct cgtattctga ctcgccccct
     2401 ggcgcagcaa agtcaggcat ttatactctt tt
//
LOCUS       DAOFQD010000271         2338 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.276, whole genome shotgun sequence.
ACCESSION   DAOFQD010000271 DAOFQD010000000
VERSION     DAOFQD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.276"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1172
                     /locus_tag="QBF46_004806"
     CDS             <1..1172
                     /locus_tag="QBF46_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDQ6676882.1"
                     /translation="DQRYLRQESLKKYSINTHYISVNPNRLAGMKWPPASFRELLDWL
                     EKSDHNARDRVVHCITTEGKKRYIFLFDIFNQDIFAIYIEFNTNAVDFSRHKRSAKKS
                     TSKLATLLAGKSVCASYKRLGVVRADMATLLSRNTQRGSKEYLSDKRIALVGCGTIGG
                     YIAELLLRNGAGCGKGLLHLFDDDIYSPSNFGRHILSSHDFGWSKALSLATKLQNSVH
                     LQTRVEGFYKQFPISIDVMKKYDIIIDATGRPPVSKRIAAIVRQIPCEQRPIIIHAFN
                     DGNGRASKVLIDDGRSCYGCMVSNPARYHNGIDSRFYNLDISSEKNKSCGSTYTPYDA
                     AVSNITASLAQQAVLSTLQPDLKWTYNEHMLEGGRSLKPQFLPHQPNCPICNEHN"
     gene            1159..1629
                     /locus_tag="QBF46_004807"
     CDS             1159..1629
                     /locus_tag="QBF46_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004960900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HDQ6676883.1"
                     /translation="MNITELVFIDDDYNHVVIMSDVVQRLHLYRQLHYASTEAGGTLI
                     GERRGKHIVITHISEPGSGDVRSRTRIERKGEHHQQKVDDLFQQSDGSLVYLGEWHTH
                     PEDFPQPSSTDMRSWRTGLKATEPMVLLIMGRKQAWCGKKHGNVIKKLEEKNNH"
     gene            1899..>2338
                     /locus_tag="QBF46_004808"
     CDS             1899..>2338
                     /locus_tag="QBF46_004808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676884.1"
                     /translation="MPQHLDIANPYVKIQKKSEQDREMESKNSSFYFDRVDFLSKSIS
                     YSTLMISCLSIVGIFLNSHWLYGIATLTFIIQLLTWFAMHFKTSYAAKAIETKRLEML
                     RGIIGEENFYRERLYIDGNAENKNGFRAERLITLIQENAYWNSI"
BASE COUNT          753 a          436 c          472 g          677 t
ORIGIN      
        1 ttgatcaaag atacctccgt caagagtctt tgaaaaaata ttctatcaac actcattata
       61 tttctgtaaa ccctaatcgt cttgctggga tgaaatggcc accggcttca ttccgcgaat
      121 tactggactg gctagaaaag tcggaccata atgctaggga cagagtcgtt cattgcatca
      181 caacagaggg aaagaaaaga tatatttttt tattcgacat tttcaatcaa gatatatttg
      241 ctatctatat tgagttcaat actaacgctg ttgatttcag taggcacaag aggtcagcta
      301 aaaaatcaac gtccaaactt gccacattgt tagctggtaa aagtgtatgc gcctcatata
      361 aacgattggg tgtggttcgc gcggatatgg caaccctcct cagcagaaat acccaaaggg
      421 gaagcaaaga atacttatca gataagcgta tagctttagt tggttgcgga accattggtg
      481 ggtatattgc agaactgtta ctccgaaacg gtgccggttg tgggaaaggt ttactgcatt
      541 tatttgatga tgatatctac agtcctagta attttggtcg tcacatttta tcttcacatg
      601 attttgggtg gtctaaagcg ctctcactcg caacaaaact tcaaaactct gtgcatttac
      661 agaccagagt agaagggttt tataagcaat tccccatcag tattgatgtt atgaaaaaat
      721 atgacattat tatcgatgca acaggccgac ctcccgtttc caagcgtatt gctgctatcg
      781 taagacagat tccctgtgag caaagaccta ttattatcca cgccttcaat gatggtaatg
      841 gacgagcatc caaagtttta atcgatgatg gccgaagctg ctatggctgt atggtttcta
      901 atccagcaag ataccataat ggtatcgatt cccgatttta caacctcgat atctcaagtg
      961 aaaaaaataa aagttgtggt agcacataca caccttatga tgcggcggtg agtaatataa
     1021 cagcatcttt ggctcaacag gcagtcttga gtactcttca gccagacctg aaatggactt
     1081 acaacgagca tatgttagaa ggtggacgtt cactgaaacc tcaatttttg ccacatcagc
     1141 ctaactgtcc tatctgcaat gaacataact gagttagttt ttatagatga tgattacaat
     1201 catgtcgtca tcatgagtga tgttgtgcag cggttacatt tatatcgtca attacattat
     1261 gccagtaccg aggcaggagg aacccttatt ggagaacgtc gagggaaaca tattgtcatc
     1321 actcacatat cagaacctgg atcaggtgat gtaagatcgc gaacacgaat agaaagaaaa
     1381 ggagaacatc atcagcaaaa agtggatgac ttatttcagc aatcagatgg ttctctagtc
     1441 tatttgggcg aatggcatac gcatcccgaa gatttcccac agccatcaag tactgatatg
     1501 aggtcttggc gaaccggact gaaagcgacc gagccaatgg ttttgcttat tatgggtagg
     1561 aaacaagcat ggtgtggtaa aaagcatggg aatgtcataa aaaaattaga agaaaaaaac
     1621 aatcattgaa cgcattcaat cttttcagat aattcaaaag attgcatatc cttcagcacc
     1681 tgtaaggtag gtggttattt tacaaaaatt agctttctca gtaacgactg agccataaca
     1741 attaaagtat taaaagttta acctcgagtt taggaaacaa gcatctgaac taaagtctga
     1801 gattactttg ccttgtagtt gatacatcgt tatgtggaaa atcatgacat gattggttca
     1861 taggaactat ttactcccca tacgttatga acatattctt gccccaacat cttgacattg
     1921 ccaatccgta tgtaaaaata caaaaaaaaa gtgagcaaga tagagaaatg gaatcaaaaa
     1981 atagtagttt ttattttgat agagttgact ttttatctaa gtctatcagc tattcaacat
     2041 taatgatttc ctgcttatcg atcgtcggga tttttttgaa ttctcattgg ctctacggta
     2101 tcgcaaccct cacttttata atacaactcc tcacttggtt tgcaatgcat tttaaaactt
     2161 cgtatgcagc taaggctata gaaacaaaaa gattagaaat gctgagaggt attataggcg
     2221 aggaaaattt ttatcgagag cggctctaca ttgatgggaa tgcggaaaat aaaaatggct
     2281 ttcgtgcaga gaggttaata acactcatac aagagaatgc atactggaac tctatctt
//
LOCUS       DAOFQD010000272         2323 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.277, whole genome shotgun sequence.
ACCESSION   DAOFQD010000272 DAOFQD010000000
VERSION     DAOFQD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.277"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..488)
                     /locus_tag="QBF46_004809"
     CDS             complement(<1..488)
                     /locus_tag="QBF46_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6676885.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     IS"
     gene            496..1167
                     /gene="tnpA"
                     /locus_tag="QBF46_004810"
                     /pseudo
     CDS             496..1167
                     /gene="tnpA"
                     /locus_tag="QBF46_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1881..>2323)
                     /locus_tag="QBF46_004811"
     CDS             complement(1881..>2323)
                     /locus_tag="QBF46_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6676886.1"
                     /translation="EQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSL
                     ISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSA
                     IQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          690 a          511 c          444 g          678 t
ORIGIN      
        1 atactgatgt accacttacc gcaggactgg ctgacagtga catttttcac aacacccgtg
       61 acctgacggc tgttccggta gcgcatccag cccagtttcg gcagaaaaat acggctgttt
      121 tcctgatcga gcttaacgcc ctgcgggtag cggaatgcat cattctgtcc ccgctttttg
      181 aatcggggaa aagcagcccg cttccggaag aagtttttgt aagcccgctc aaggtctttc
      241 agtgactgtt gcaatggctg tgagggagaa tctttaagcc attgcgtttc agtggcattt
      301 ttccactcaa ccagccagga agccattttc ccgtaaggga tgtatttatt accggcctca
      361 tgattctcat tctgacgtgc cagagcacga ttgaaaacga aacgacatgc gcctgcgaag
      421 cgcctcattt cacactcctg ttgaccacct ggtcttaact ggaatttaaa tgcctgtaat
      481 cgtttcacag gtcaaatgat actttggtct gtgaaaaaag aaaccgatat ccggcgtgga
      541 aggcattgtg ttttcctgat gcatgttcac ctgctctttg tcaccagata ccgacgccag
      601 atttttgacc atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgcttat
      661 tttgaagctg aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac
      721 tatcctccca aactagcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg
      781 tgactgcgac gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg
      841 agtcctggct attttgccag tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac
      901 gttgaacaac agcaaacacc tggtcaggtg gaaaaccgcg ccttatatcc ccgccctgaa
      961 ggacggggtt ttacggcgca ccggataagc ctggagatta tctacaccag catggacatc
     1021 attgtactat gccccgtaca tgccccatca catcctcaac atcaaagtat tagagccttg
     1081 caaaccatta tgcgtctcag tttgtctcat ctatcttacg caaaaaaaaa tagaactaat
     1141 atcaagcggc gctatattgc gtattaaata aaaccacctc aattacatta ataaatctaa
     1201 acaaacatca tgcaaataat ttccatggtt atttctaggc tatttaaatt aaataaataa
     1261 attatgagtc aatcataaat aacaaccttt aatataattt aactaccaaa tttatattcg
     1321 ttcacaaaat agtcatgcat tgtgtgcaat agaaacagtt cagataaaga tagggagcta
     1381 cttcacaatt taaatgagaa actaaaactt atatcttgaa ataatctcat tgattggatg
     1441 aatatttgta gtgcagtgac atcattttta aataatagtt caaataaaag ggctcacgat
     1501 gaaaaaatta acagtggcaa tttctgctgt agctgcatct taacgtataa actagattta
     1561 ataacaacga actagattta ataacaacga tatccgtacc tatccggata tctaatgtca
     1621 taagcatccc tttaattatc ccctcaacct cttgtaactt tcccactctt gaactccaca
     1681 tagtccaaac gccggatgca aaaaaatcac acgtgcattt ctgaaattgt gatggaggga
     1741 gatagtatca attatatgtg cttataaacg tccccgatta gcgcagaagt caccggattg
     1801 ttcaggctcc aataggttga gtatgccgta tttattttcc aaaataaaaa tcgatgctgt
     1861 tgctccttca taaaatactt ttactctctg gacgcttttc ttagctctct tggtgttatc
     1921 ttaaaacgat tatggaacct ttcagtaaaa cgagaaggac acttgtaacc attttccctg
     1981 gcaatctcgc tgatgggttt tactgtcgtt tgtatagcag acaacgcatt atttaacctc
     2041 acgtcgtcca atatactttg aaaacttact ccctcgcttg ccagacggcg atgcaatgtg
     2101 gaaacagaaa tgtagagata tctcgctacc ttgtttgctg tccattttgt tccaggttcg
     2161 gatagcatta ggttataaca acgacttatt aatgattgtt tactatctga taaaagtaaa
     2221 caattaacat gatgtgctcc taatgaaagt agaacgccca ttgctaagtg ttcctgaatt
     2281 tgcgttgaga agcctcggga aatagatgtt tttagttgct ccc
//
LOCUS       DAOFQD010000273         2316 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.278, whole genome shotgun sequence.
ACCESSION   DAOFQD010000273 DAOFQD010000000
VERSION     DAOFQD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.278"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..787)
                     /locus_tag="QBF46_004812"
     CDS             complement(<1..787)
                     /locus_tag="QBF46_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HDQ6676887.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAI"
     gene            complement(1084..1470)
                     /locus_tag="QBF46_004813"
     CDS             complement(1084..1470)
                     /locus_tag="QBF46_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6676888.1"
                     /translation="MSQRTSSLLYSHQPPHAKCVLLGQLGWHYLFPASHLSRDPVDGA
                     LRRHHLDPTTLQKAVRATARQLQLGKPVTCHTLRHSFATHLLARGADIRTVQEQLGHS
                     DVRTTQIYTHVLQNGANGVRSPLSDL"
     gene            complement(1570..2274)
                     /locus_tag="QBF46_004814"
     CDS             complement(1570..2274)
                     /locus_tag="QBF46_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDQ6676889.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          507 a          618 c          638 g          553 t
ORIGIN      
        1 tgatggccgt tttgttcttg cgtggatgct gtttcaaggt tcttaccttg ccggggcgct
       61 cggcgatcag ccagtccaca tccacctcgg ccagctcctc gcgctgtggc gccccttggt
      121 agccggcatc ggctgagaca aattgctcct ctccatgcag cagattaccc agctgattga
      181 ggtcatgctc gttggccgcg gtggtgacca ggctgtgggt caggccactc ttggcatcga
      241 caccaatgtg ggccttcatg ccaaagtgcc actgattgcc tttcttggtc tgatgcatct
      301 ccggatcgcg ttgctgctct ttgttcttgg tcgagctggg tgcctcaatg atggtggcat
      361 cgaccaaggt gccttgagtc atcatgacgc ctgcttcggc cagccagcga ttgatggtct
      421 tgaacaattg gcgggccagt tgatgctgct ccagcaggtg gcggaaattc atgatggtgg
      481 tgcggtccgg caaggcgcta tccagggata accgggcaaa cagacgcatg gaggcgattt
      541 cgtacagagc atcttccatc gcgccatcgc tcaggttgta ccaatgctgc atgcagtgaa
      601 tgcgtagcat ggtctccagc ggatagggcc gtcggccatt gcccgccttg ggataaaacg
      661 gctcgatgac agcggtcata ttctgccatg gcagaatctg ctccatgcgg gagaggaaaa
      721 tctcttttcg ggtctgacgg cgcttagtgc tgaattcact atcggcgaag gtgagttgat
      781 ggctcatgat gtccctctgg gatgcgctcc ggatgaacat gatgatctca tatcaggaac
      841 ttgttcgcac cttccctgtc tatcgcacat ttttgcaaat cccgtcgatt atttatttcc
      901 atatgaaact gaaatccgta gcatagtttg acgaaaagaa cagaccatca ggatgaaaat
      961 ggcccgggta acaagccacc acctgaaacg gatgtcagcc tctaggagct gtaacctaag
     1021 ggcggtagcg tgcctgcgtc aggccacttg ttctgaaaaa ccgagcgtcc cgccccccaa
     1081 ccattacaga tcagacaaag ggctacgtac cccattggcg ccgttttgca gcacatgggt
     1141 ataaatttgt gtggttcgaa catcagaatg gcccagttgc tcctgcacgg ttcgaatatc
     1201 cgccccccgc gccagcaaat gggtggcaaa ggaatggcgc aaggtatggc aggtgaccgg
     1261 ttttcccagc tgcaactgac gcgccgtggc acgaaccgct ttttgcaggg tggttggatc
     1321 cagatgatgt cgacgcaagg ccccatcgac aggatcgcga ctcagatggg atgccggaaa
     1381 caggtagtgc caacccagct gccccagcaa aacacatttc gcgtgtggtg gctggtggct
     1441 atataacaaa gaacttgtcc gctgacttat aggggttgta agccggaacc gccgaaattt
     1501 ccgttagcca gaggggcggc actgttgcaa agttagcgat gaggcagcct tttgtcttat
     1561 tcaaaggcct tacatttcaa aaactctgct taccaggcgc atttcgccca ggggatcacc
     1621 ataataaaat gctgaggcct ggcctttgcg tagtgcacgc atcacctcaa tacctttgat
     1681 ggtggcgtaa gccgtcttca tggatttaaa tcccagcgtg gcgccgatta tccgtttcag
     1741 tttgccatga tcgcattcaa tcacgttgtt ccggtactta atctgtcggt gttcaacgtc
     1801 agacgggcac cggccttcgc gtttgagcag agcaagcgcg cgaccatagg cgggcgcttt
     1861 atccgtgttg atgaatcgcg ggatctgcca cttcttcacg ttgttgagga ttttacccag
     1921 aaaccggtat gcagctttgc tgttacgacg ggaggagaga taaaaatcga cagtgcggcc
     1981 ccggctgtcg acggcccggt acagatacgc ccagcggcca ttgaccttca cgtaggtttc
     2041 atccatgtgc cacgggcaaa gatcggaagg gttacgccag taccagcgca gccgtttttc
     2101 catttcaggc gcataacgct gaacccagcg gtaaatcgtg gagtgatcga cattcactcc
     2161 gcgttcagcc agcatctcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg
     2221 tacggcccac agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag
     2281 ctccatcagc aaaaggggat gataagttta tcacca
//
LOCUS       DAOFQD010000274         2300 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.279, whole genome shotgun sequence.
ACCESSION   DAOFQD010000274 DAOFQD010000000
VERSION     DAOFQD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.279"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..486)
                     /locus_tag="QBF46_004815"
     CDS             complement(<1..486)
                     /locus_tag="QBF46_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDQ6676890.1"
                     /translation="MTMSDLSATGAKPQRVRVFSQSVAMAFEVNLDKSRSADNSAFVT
                     ETEKYYLSLQFAPPSDNREYGWNSEGSILMKLSQNEAMALASVFLRIKPTLKFEKRKT
                     THRTHQAYKNINIGPNDRGGLLVSASIVPVKMGTFKPLNYNLPVTQMDCVSTGLFLLG
                     FL"
     gene            complement(483..917)
                     /locus_tag="QBF46_004816"
     CDS             complement(483..917)
                     /locus_tag="QBF46_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="HDQ6676891.1"
                     /translation="MKVNFKKVIPLLMVGLTVAGSYSIFNAKPSKPDLYDFTGKVLKT
                     TSVFQPCDKESTPSLNIQIADNGSVHINGVASKVTFVERVPGNEIAVKCTGLQVKNSR
                     LVHTSSYTMIISEGKGGFVISDLTHTQDNEVMSGTWFFKKRV"
     gene            complement(907..1359)
                     /locus_tag="QBF46_004817"
     CDS             complement(907..1359)
                     /locus_tag="QBF46_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transfer repressor"
                     /protein_id="HDQ6676892.1"
                     /translation="MLSRNSLIHGLRRDQLIEVLSISEFPVVLVENPFIQPKSTGKKP
                     VIFNIDEFHVSIAPISSLKYDWEWAPIDTILIEVVIPPAEADLVSAENDFLRDAGIGH
                     IQCEPGGVSIRRTVTFVGGITADNLLYQLRLMCVSALHLIGEELEDES"
     gene            complement(1924..>2300)
                     /locus_tag="QBF46_004818"
     CDS             complement(1924..>2300)
                     /locus_tag="QBF46_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676893.1"
                     /translation="SFMLDSKFNELFSMINGCDFSKATFSTTSQSDVSETLKAERVRD
                     QRLELIPKELTVNPKPETLEILTPIESAKKNDSGLNTSHGSAAVSASQSENTVLSEHA
                     QIQGVTRKPKKKANANLANLAK"
BASE COUNT          681 a          505 c          432 g          682 t
ORIGIN      
        1 caggaacccc aacaagaaca agcctgttga aacgcaatcc atttgtgtta cgggaaggtt
       61 ataatttaaa ggcttaaacg tccccatttt aaccggtaca atgcttgcgc ttaccaaaag
      121 gcccccacgg tcgttgggtc cgatattgat atttttgtag gcttggtgag tgcgatgcgt
      181 ggtcttacgc ttttcaaatt ttagtgtcgg cttaattctc agaaaaacag atgccagcgc
      241 catggcttcg ttttgggata gtttcatgag aatgctgccc tctgaattcc atccatattc
      301 cctattgtca gacggaggag caaactggag agacaggtaa tacttttcag tctcagttac
      361 aaacgccgaa ttatcagcag agcgactttt gtcgagattt acttcgaatg ccatcgcgac
      421 agattggctg aatactctga cccgttgagg cttcgcacca gttgctgaca gatcgctcat
      481 tgtcatactc tcttcttaaa gaaccatgta ccggacataa cttcattatc ctgcgtgtgc
      541 gtcaaatccg aaataacaaa accacctttt ccttccgaaa ttatcatggt gtaagagctc
      601 gtatgcacaa ggcgggaatt tttgacttgc agacccgtac acttaactgc aatttcatta
      661 cccggaactc tctcaacaaa agtaactttt gatgcaacgc cattgatatg tacgcttccg
      721 ttatctgcta tctggatgtt caacgaaggt gtggattctt tatcacaagg ttggaaaaca
      781 gaagtggttt taagaacttt gccagtaaaa tcgtacaagt caggcttcga cggttttgcg
      841 ttaaagatcg agtaagaccc cgccactgtt aaaccaacca ttaaaagggg gattaccttt
      901 ttaaaattaa ctttcatcct ccagttcctc tccaatcaag tgtaaagcac ttacgcacat
      961 aagcctgagt tgatacagca aattgtcggc ggtgatcccc ccaacaaatg tcactgtacg
     1021 gcgtatcgaa acgcctccag gctcgcattg aatatggcca atacctgcat cgcgaaggaa
     1081 atcattctcg gcgcttacca ggtctgcttc agcaggcgga atgaccactt cgatgagtat
     1141 tgtatcaatt ggagcccact cccagtcgta cttaagcgat gaaattgggg caatggaaac
     1201 atgaaattca tcaatattaa aaatgactgg ttttttgcct gttgatttag gctgaatgaa
     1261 gggattctcg accaggacaa ctgggaattc agaaatagac agaacttcga ttaactgatc
     1321 tcgacgtaaa ccgtgtatta gtgagttgcg tgataacatt cagtcagcct ttattataat
     1381 aatcagatag ttacacatga tcccgctagt tccttaccat cttcccgctt gcagcgtagt
     1441 aaccattaag atcttatggg ttagggtaac aaaaaaatct aacttaacca gtaaagcata
     1501 gagatttttt acataaaata ataaataagt accaatttta ttaagatatt tcctatatca
     1561 gccaatactt taggaaaaat ctgtacccgt tttaacacct atacatacat cgtgcaaaca
     1621 aagaacagta aacggggcct aaaccacctt ttttagtggt ttacctaacc aaaaataatt
     1681 tatccaaaat cgtaactggt tcgacctgaa tcaacccacg cgaattttaa tgatttctta
     1741 gaactggctc tataattctt caaactggct taactggaga tgtgacaacg agggattcat
     1801 ttagtacatg aaaccggttt accattcccc ctcacctgtt aataatatac caaaaaaaag
     1861 ccagctggta agctgacttt ttattaatag ataactcaat ctaatcaatt gatattacta
     1921 tacttacttc gcaagatttg cgaggtttgc attggccttt ttcttcggct tccttgtcac
     1981 accttggatt tgcgcatgtt cactcagcac cgtgttttcg ctttgtgagg cgctaacagc
     2041 agcagaacca tgactagtat ttaaacctga atcattcttt tttgcgctct caatcggcgt
     2101 taaaatctct aaagtctcag gtttgggatt tacagtcagt tctttaggta ttaattccag
     2161 ccgctgatcc cttaccctct ctgcctttaa tgtttcactg acatcagatt gactagtagt
     2221 tgaaaaagtt gccttagaaa aatcacagcc attaatcatt gaaaataatt cattgaattt
     2281 cgaatcaagc ataaagctta
//
LOCUS       DAOFQD010000275         2297 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.280, whole genome shotgun sequence.
ACCESSION   DAOFQD010000275 DAOFQD010000000
VERSION     DAOFQD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.280"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..867)
                     /locus_tag="QBF46_004819"
     CDS             complement(<1..867)
                     /locus_tag="QBF46_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDQ6676894.1"
                     /translation="MAYLDPYQRPAPDRFVRRGLFITACIAALMLLWQFLPAIEAWFS
                     PREAAERTVMARGDLAADEKTTIELFEKSRASVVYITTAQLVRDVWTRNVFSVPRGTG
                     SGFIWDDAGHVVTNFHVIQGASEATVKLADGRDYQAALVGTSPAHDIAVLKIGVGFKR
                     PPAVPVGTSADLKVGQKVFAIGNPFGLDWTLTTGIVSALDRSLPGEAGGPAIDHLIQT
                     DAAINPGNSGGPLLDSAGRLIGINTAIYSPSGASAGIGFAVPVDTVMRVVPQLIKTGK
                     YIRPALGIEVDEQ"
     gene            complement(892..1857)
                     /locus_tag="QBF46_004820"
                     /pseudo
     CDS             complement(892..1857)
                     /locus_tag="QBF46_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196690.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
     gene            complement(1835..2287)
                     /gene="psiE-GI"
                     /locus_tag="QBF46_004821"
     CDS             complement(1835..2287)
                     /gene="psiE-GI"
                     /locus_tag="QBF46_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance protein PsiE-GI"
                     /protein_id="HDQ6676895.1"
                     /translation="MAIMSFYERFEQVVALVLSAVIAVIIVVSLFQLISIVFTLLVLD
                     AFNPLDHEVFQSVFGMIMTLLIAMEFKHSIVRVALRRDSIIQVKTVVLIALIALSRKF
                     VILDSNASPAKIAALASATLALGATYWLLRKRGDRAAETSEHDPSSSQ"
BASE COUNT          435 a          719 c          784 g          359 t
ORIGIN      
        1 ctgctcatcc acctcgatgc ccagtgccgg acggatgtac ttgccggtct ttatgagttg
       61 cggcaccacg cgcatgacgg tatcgaccgg caccgcaaag cctatgccgg ccgaggcgcc
      121 agacggactg tagatggcgg tattgattcc gatcagccgc ccagccgaat cgagcagcgg
      181 gccaccggaa ttgccggggt tgatggcggc gtcggtctgg atcaggtgat cgatggccgg
      241 gccgcccgct tctcccggca acgagcggtc gagcgcggag acgataccag tggtgagcgt
      301 ccagtccagg ccgaaggggt tgccgatagc gaacaccttc tgacccacct tgaggtcggc
      361 actggtgccg accggcacgg ccggcgggcg cttgaaaccg acgccgatct tgagcacggc
      421 gatgtcgtgc gcggggctcg tccccaccag cgcggcctgg tagtcgcggc cgtcggccag
      481 tttgacggtg gcttcggacg cgccctggat gacgtggaag ttggtgacga cgtggccggc
      541 gtcgtcccag atgaagcccg agccagtgcc gcgcggcacg gagaacacgt tgcgcgtcca
      601 gacatcgcgt actaattgcg cggtggtgat gtagaccacc gaggcgcgcg atttttcgaa
      661 cagttcgatg gtggttttct cgtccgccgc cagatcgcca cgcgccatca cggtgcgttc
      721 tgctgcctcg cgcggactga accatgcctc gatggcgggc agaaactgcc acagcagcat
      781 gagcgcggca atgcaggcgg tgatgaagag cccgcgccgg acgaagcggt ccggcgcggg
      841 gcgttggtac gggtcgagat aggccatgag acgtctcctg ttgatgggca atcagcgcca
      901 cagcccgctc gggcgccagc gcggtgggcg cgtcgccaaa gcggattccg gcaaaaaatg
      961 gggcgcgcgg agtggcaacg ctgatgctgg cccaggcgcc aacgtcagca ggcgtgcgat
     1021 gcgctcttgc gttgccggac gcgtacgcaa gcaggagtgc tcgggattgc cccatcccgg
     1081 ccatagctag gcacgccagg agcggctgac ccgctcgatt tttgccagca cggacgccag
     1141 cccctgcggg tcgccagtca gttccgccgc aaggcggtca gcgtcgaact cacgcacgcg
     1201 agacaagccg agctgtgcga gcagggccag ctggggcgat gcggccaata acaatagacc
     1261 aggccaatag acctccgccg cgccaaccag ctgcgcgggc aggctgagct ggatcgcgat
     1321 ctgtcccata agggccagta ggctcgtgag gcgactgtcg gaatccgcca gccccatgac
     1381 acgcagatcc tcattagcga tgtgcgcgac ctcgtgcacg agcacacccg ccagctcgcg
     1441 tgggctcagg ctgcgcagca ggccatcggt gagggcgatc gatgcctcct gcttcgatcc
     1501 ggtggcgaag gcgttgacga cggcactggg cacatagtgc ggcaccggcg tggcgggcaa
     1561 accggcacgg gcgggcagct cgcgcaacag ggcccacatc ttgtgggctt cttgcgggtg
     1621 caaggcccgt acgcggtaca ggcgcaagct cagcgccgac gcggctaccg gttccagcag
     1681 caacgcacct gcaatggcaa acagcgcgag ccacaggcag ccttccccaa acggcagtct
     1741 ccctgctgcg gctgcgatcc cgaccagggt caggaccaac agtccggtct gcaggcggtt
     1801 gagccagcgg tgttgcaaga gcgccgtgcg cggatcactg ggatgatgac gggtcatgct
     1861 cagaggtctc ggcggcgcgg tcgccgcgct tgcgcagcag ccagtaggtc gcacccagag
     1921 ccagcgtggc gctagccagc gcggctatct ttgcggggct cgcgttggag tcgaggatca
     1981 cgaacttgcg ggagagtgcg atcagcgcga tcaggaccac cgtcttgacc tggatgatgc
     2041 tgtcccgacg cagcgccacg cgcacgatgg aatgcttgaa ctccatcgcg atcaagagcg
     2101 tcatgatcat gccgaacacg ctctggaata cctcgtggtc caggggattg aaggcatcta
     2161 gaaccagcag cgtgaagacg atggagatga gctggaacag cgacaccacg atgatgacgg
     2221 cgatcaccgc cgacagcacg agcgcgacga cctgctcgaa gcgctcgtaa aaactcatga
     2281 tggccaattg gtcacgt
//
LOCUS       DAOFQD010000276         2288 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.281, whole genome shotgun sequence.
ACCESSION   DAOFQD010000276 DAOFQD010000000
VERSION     DAOFQD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.281"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..336
                     /locus_tag="QBF46_004822"
     CDS             <1..336
                     /locus_tag="QBF46_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HDQ6676896.1"
                     /translation="TNTYYTGCSTTIEKFKEITFEAAVSYVPGQRLTLIAEKTNTGSD
                     CGRERGVSKKEFLMSEVNSGYYTVSVPESTAYHGIHHMKFRIQDSHGNIVEDDKCVGV
                     IVSHPILLN"
     gene            632..1477
                     /locus_tag="QBF46_004823"
     CDS             632..1477
                     /locus_tag="QBF46_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676897.1"
                     /translation="MNNWSPEHTKDIKSWFKIDTYRKFEDLSLIQFYHEIWARNLFFK
                     EYREEFESRTLMGYFSKIFSGNPFLIEEGQLGYMTPANKLFQPPHFFLTTLDRLAETS
                     IIAMQRGGFLWHEGDNYSINAELREESLSDIMPDQFTRTVMFEIDLASGTDEEIAESL
                     KAALPQWRKVKGIDENPLESVRFGYGTIKKLISYRVIPMLDILVWAAVKKIRVSDDRL
                     SRLLYTDDDEESEMRQSSQIKDTDRPLALKSCTTDFIRQFHYFINKNSHLKQMKVSDV
                     MKLSD"
     gene            1754..1960
                     /locus_tag="QBF46_004824"
     CDS             1754..1960
                     /locus_tag="QBF46_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDQ6676898.1"
                     /translation="MKNQATRLIRLPEVLERTGYGKAWIYRLISEGRFPAPVKIGVRA
                     VAFVESEVDEWIQSVIETSRIKVA"
     gene            1982..>2288
                     /locus_tag="QBF46_004825"
     CDS             1982..>2288
                     /locus_tag="QBF46_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015462360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676899.1"
                     /translation="MNIEEFMSEENYMCNLGEDLFGKIFEPGAIYDLPDNEFNRKIVY
                     WLSQYLVGNLREPLDAIFELNIFDQFYVYETWFSLIKCPVEMKSLSKRIIQYHIGLRA
                     "
BASE COUNT          715 a          425 c          478 g          670 t
ORIGIN      
        1 actaatactt attatactgg ttgctcaaca acaatagaga aatttaaaga aataactttc
       61 gaagcagcag tgagttatgt tccagggcaa agattaactt taatagctga aaaaacaaat
      121 acaggtagtg attgtggtag agaaagaggc gtaagtaaaa aagaattttt aatgagtgaa
      181 gtaaattccg gttattacac tgttagtgtt cctgagtcaa cggcatatca tggaatccat
      241 catatgaaat tcagaataca agatagtcat ggtaatatcg ttgaagatga taaatgtgta
      301 ggagttatcg tttcgcatcc tattcttctc aactgaaatt gtctttcatg attatgcctt
      361 aactttattt tttccatgta tgcctgattg acttggggga agcacggagt gtttgaagtg
      421 agatctgtaa cccatcgatc tggcatctgg catctggctg tagagcttgg caagttccat
      481 caaaagataa caaatctctc agttcaactc ccagttggta gctgaactgc gtaagcagga
      541 tctgctttcc accgattagt gtatacggta gtttttcaac ataactgaat tgtacccgta
      601 gggcagtacc agcataggtg gagaatgaat tttgaacaat tggtcaccag agcatacaaa
      661 agacatcaag agttggttta agattgatac atatcgcaaa tttgaagatc tctcattgat
      721 ccagttctac catgagatat gggcccgaaa cttgttcttt aaggagtatc gagaagagtt
      781 tgagagcaga actcttatgg gttacttctc aaagattttc agcggcaacc cttttttaat
      841 cgaagaaggg caactggggt acatgactcc tgctaacaaa cttttccagc ctccccattt
      901 ttttctaaca accctggatc gtctcgctga aacgagcatt attgcaatgc aacgtggagg
      961 ttttctttgg catgaaggtg acaattattc tataaacgca gagcttcgag aggaatcact
     1021 ttcagacatt atgccagatc agtttacacg aacagtcatg ttcgagatag atttggccag
     1081 tggcacagac gaagagattg cagagtcgct taaagctgca ttaccgcaat ggcgtaaagt
     1141 taagggtatt gatgaaaacc cattggaatc tgttcgtttt ggatacggaa ctatcaagaa
     1201 actcatcagc tatcgtgtaa tacctatgct ggatatcctg gtgtgggcag ccgttaaaaa
     1261 aatccgtgtc tctgacgaca ggttatccag actactatat acagacgatg acgaagaaag
     1321 cgaaatgagg cagtccagcc aaatcaaaga taccgacagg cctttggctc ttaaatcctg
     1381 cacaactgac tttatccgac aattccacta cttcataaac aaaaatagcc atttgaagca
     1441 aatgaaagtc tctgatgtaa tgaaactatc ggattagtat tttatctata actgtctgtt
     1501 ttaatgtgaa aaacaaaaag catcattgta aatgatgctt tttgttgcgc tttttacttt
     1561 aaaccagcca ttttgccacc tgctataaaa tagggtaatt tcagctactc tcaagttccc
     1621 ctgttttgta cggtaccaac ttcgagaaaa ccatcctgca tagcagcgaa gaacctccgc
     1681 ttcaggagct tcactccttt cttttcccaa ctgttcatat agtgatttca ccgaaacgac
     1741 aagggataac gaaatgaaaa atcaagcaac ccgcctcata cgcttaccag aagttctcga
     1801 acgaactggc tacggaaagg cctggatcta tcgtctgatc agtgaaggtc gatttccggc
     1861 accagtgaag atcggagttc gtgctgttgc ttttgtcgag agtgaagttg acgagtggat
     1921 tcaatcagtt atcgaaacaa gcagaataaa agttgcttaa ttaaatctgg gagagattgt
     1981 tatgaatatc gaagaattta tgagtgaaga aaactatatg tgcaatttag gcgaagatct
     2041 ttttggtaag attttcgaac ccggagcgat ttatgactta cctgataatg aatttaacag
     2101 aaagattgtt tactggttaa gccagtattt agttgggaat ctgagagaac cgctagatgc
     2161 gatttttgag cttaatattt tcgatcagtt ttatgtctat gaaacatggt tctcgctaat
     2221 taaatgccct gtagaaatga aaagtctttc aaagcggata atccaatacc atattggtct
     2281 gagagctc
//
LOCUS       DAOFQD010000277         2286 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.282, whole genome shotgun sequence.
ACCESSION   DAOFQD010000277 DAOFQD010000000
VERSION     DAOFQD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.282"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..509)
                     /locus_tag="QBF46_004826"
     CDS             complement(<1..509)
                     /locus_tag="QBF46_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676900.1"
                     /translation="MKKLPGSLEIKLHEKLSKNDILNVLAEHMTMLEETFGIEEFKLY
                     SFLECYIDSKKQSFYHEEHNTVVNSFDLQGNEKLENTAKIISKDGNKRIVSFDKNIDV
                     DRIRLTVGSIQKHNPYRYWSNNIQVIPSSVVSNIIQDKESQLQLERDKLYFLNQLRRK
                     QEEEQRRKER"
     gene            complement(529..1263)
                     /locus_tag="QBF46_004827"
     CDS             complement(529..1263)
                     /locus_tag="QBF46_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011093221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDQ6676901.1"
                     /translation="MNTNDTELLRLFETLTTEQKIDIARHLLQNKDIKSTTVIATEED
                     KAISFAQPYVFAFTSNFRVFSDLDISKNEFRVLTYILEYMEFGNLINLSQSSLCKALN
                     IASGNMSNIFKKLKQKGILVEHHGHLYINSNIFAKGMSHQLDEKRKEHLSNARNVQAE
                     IEQYPDGYQNLSQQERRGLALETVNQNVYTDAFIFSKKKKNNAVPNAKNSIKIDKPRK
                     VYKRTKENEANRQDLVNITNHLKAVS"
BASE COUNT          680 a          337 c          402 g          867 t
ORIGIN      
        1 cgctctttac gcctttgttc ctcttcttgt ttacgtctca gttggttgag gaagtaaagt
       61 ttatctcttt ctaattgtag ttggctttct ttatcttgaa tgatgttgct gactactgat
      121 gatggaatga cttgaatatt gttagaccaa tatcgatacg gattatgttt ctgaatactt
      181 ccaactgtta aacgtattct atctacatcg atatttttat caaaagagac gattctttta
      241 ttaccatctt ttgaaattat ttttgctgtg ttctcaagtt tctcattacc ttgtaagtca
      301 aatgaattta caacagtatt atgttcttca tgataaaagg actgtttttt actatcaata
      361 taacattcaa gaaaagaata gagtttgaac tcttcaatgc caaatgtttc ctcaagcatc
      421 gtcatatgtt ctgctaagac attcagaatg tcgttttttg atagtttttc atgaagtttt
      481 atttcaagtg aaccgggtag ctttttcatt ttcattcctt gttattattt aggaaacagc
      541 ttttaagtgg ttagtgatat taactaaatc ttgtctattt gcttcatttt cttttgttcg
      601 tttatatacc ttgcggggtt tatctatctt gatactgttt ttagcattag gaactgcatt
      661 gttctttttc ttcttgctaa aaataaaagc atctgtataa acattttgat ttactgtttc
      721 gagggctaat cctcgtcttt cctgttgaga aagattttga taaccatcag ggtattgttc
      781 aatctcagcc tgaacatttc ttgcattact caaatgctct ttacgttttt catcaagttg
      841 atgactcata cccttggcaa aaatattaga gttgatatac aggtgtccat gatgttcaac
      901 gagaatacct ttttgtttta gctttttgaa tatgtttgac atattaccgg atgcgatatt
      961 caacgcttta cagagtgatg actgtgagag gttgattagg tttccaaact ccatatattc
     1021 taagatataa gtcaacacgc ggaattcatt cttagatata tccagatcag aaaaaacacg
     1081 aaagtttgaa gtaaaagcaa aaacataagg ctgtgcaaat gagatcgctt tatcttcttc
     1141 tgttgcaata acggtggttg attttatatc tttgttttgt agcaagtgtc ttgcgatatc
     1201 aatcttttgc tctgtggtta atgtttcaaa taagcgaagt agttcagtgt cattagtatt
     1261 catattattc tctcttatag ttcttattat gcctatttaa tactatgcag aaaaatgaaa
     1321 gtcaatagtt aaactataaa taaaatagaa cttttattat tatgatgttg ctatatgtta
     1381 aaaaaactat gtttaaacat agattccgct atgtttaaac ataggagggt tctatgttta
     1441 aacataggtg tctgctatgt ttaaacatag gaaaaaaagg tgtgaagccc cgattttatt
     1501 gggctgggag agtgcttata attaaataat tagtaagaag ttaagagagt ccagttttcc
     1561 ttttatctta tgtgcagagc gttttacgta aaaacatatg gttttcttgg ggtgtatttt
     1621 cagcgaagtg attgtttttt acaatcacga agcgctgctt tctgttgcta aattgataat
     1681 catagttttg ttgtaaagaa tggaatttac ttttaactgt attagcggct actgccgcaa
     1741 ttctttatgg ataaatctat cattaacagg gatgaaccct gtttcagcaa tgtgattgtt
     1801 tctgacaatc actcagtgct gctattgagg atatagtttt actatgtgtt taagagcaaa
     1861 tgctatagcc tcgatggatt tatcattaat atagaaaaag ggcgaagcca tgagaggagg
     1921 ctcataataa aagatctgtt ttcaatatga tgtcttaatt aagtttaagg taaagctagt
     1981 atataaaatg tcttaaaaat gaaatcgtac atgaagtggt aaaattaatt gtactaatct
     2041 tacattagcg gctactgccg caattcttta tggataaatc catcgttaac aggggtgaac
     2101 cctgtttcag cagtgtgatt gtcttgagca atcactaagt gctgcttcta tgaatatagt
     2161 tttcctatgc gtttaagagc aaatactata tcttttagtg ctaatgctgt tatgaaatat
     2221 aggatcgaaa atatttaatc atgaataaaa gaatagatgt tgatcctttt ggctttaccc
     2281 taaaac
//
LOCUS       DAOFQD010000278         2202 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.283, whole genome shotgun sequence.
ACCESSION   DAOFQD010000278 DAOFQD010000000
VERSION     DAOFQD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.283"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            196..2181
                     /gene="yjcS"
                     /locus_tag="QBF46_004828"
     CDS             196..2181
                     /gene="yjcS"
                     /locus_tag="QBF46_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDQ6676902.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPHKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMGNGDNLNLTLNDSVLNYRKTLQPQANASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          583 a          571 c          548 g          500 t
ORIGIN      
        1 acctcatatt atttacagag ataataaatt cgcgtacttt tccctcttca gcacaaatgc
       61 ctaataaaat ggcgcgttca gctcacactt ccacttatta aagtcgcaat ttcccaatta
      121 tgataacgcc agctttaaat gacgtatttt gcatggatta catatttacg acataaatga
      181 taaatggaga tttatatgaa taactctcgg ttattccgtt tgagcaggat tgttattgcg
      241 ttaactgccg ccagcggcat gatggtaaat accgctaacg cgaaagagga agcgaaagcc
      301 gccactcaat atacccaaca ggttaatcag aattacgcca aatcattacc gtttagcgat
      361 cgtcaggatt ttgacgatgc ccagcgtgga tttatcgccc cgctgctgga tgaaggtatt
      421 ctgcgtgatg cgaacggtaa agtttactac cgcgcggacg attacaaatt tgatattaat
      481 gccgcagcgc cggaaaccgt aaaccccagc ctgtggcgtc agtcgcaaat caacggtatt
      541 tctggcctgt tcaaagtcac cgataaaatg tatcaggtgc gcggccagga tatctctaac
      601 attacgttcg ttgagggcga gaaaggcatt attgttattg acccactggt gacgccacct
      661 gccgcaaaag ccgcacttga cctttacttc cagcatcgtc cgcataaacc gattgttgcc
      721 gttatctaca ctcacagcca cactgaccac tatggtggcg tgaaaggtat tatctctgaa
      781 gccgatgtta aatccggcaa agttcaggtg attgcccctg caggctttat ggacgaagcc
      841 atcagcgaaa acgtgctggc gggtaacatc atgagccgcc gtgcgctcta ctcttacggt
      901 ctgttactgc cgcacaacgc gcaaggcaac gtgggtaatg gccttggcgt gacgctggca
      961 acgggcgacc cgagcattat tgcaccgact aaaactatcg tcagaactgg cgagaagatg
     1021 attattgacg gcctggagtt tgacttcctg atgaccccag gtagcgaagc gccagccgaa
     1081 atgcacttct atattccggc cctgaaagcc ctgtgtaccg ccgagaacgc cacgcatacc
     1141 ctacacaact tctacactct gcgcggcgcg aaaacccgcg ataccagcaa gtggaccgag
     1201 tacctgaacg aaacgctgga tatgtggggt aacgacgcgg aagtcctgtt tatgccgcac
     1261 acctggccgg tctggggcaa taagcatatc aatgattata ttggtaaata tcgcgatact
     1321 atcaagtaca ttcacgacca gaccctgcac ctggcgaacc agggctacac catgaatgaa
     1381 atcggcgaca tgattaagct gccgcctgca cttgccaata actgggccag ccgtggctat
     1441 tacggttctg tcagccacaa cgcccgcgcg gtgtataact tctatcttgg ctattacgac
     1501 ggtaacccgg ctaacctgca tccgtatggt caggtggaga tgggtaaacg ttacgtgcag
     1561 gcgctgggcg gttctgcccg tgtcatcaac ctggcgcaag aagcgaacaa gcaaggtgat
     1621 taccgctggt cggcagaact gctgaaacag gtgattgccg ccaacccggg tgaccaggtc
     1681 gcgaagaatc tgcaagcgaa taactttgaa cagctgggct atcaggccga gtccgccaca
     1741 tggcgcggtt tctacctgac cggcgcgaaa gagctgcgcg aaggggtgca taagttcagc
     1801 cacggcacca ccggttcccc ggacaccatt cgcgggatgt cggtcgaaat gctgttcgac
     1861 tttatgtccg ttcgcctcga tagcgcgaaa gccgcgggta aaaatatcag cctgaacttc
     1921 aatatgggca acggcgataa cctcaacctg acgctgaacg atagtgtgct taactaccgg
     1981 aaaacgctgc aaccgcaagc caacgcctct ttctacatca gccgtgaaga tctgcacgcc
     2041 gtgctgaccg gacaggcaaa aatggcggat ctggtaaaag cgaagaaagc caaaattatt
     2101 ggcaatggcg cgaaactgga agaaattatt gcctgtctgg ataatttcga tttgtgggtg
     2161 aatatcgtaa ccccaaatta atgccactcg cttttggggg cg
//
LOCUS       DAOFQD010000279         2186 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.284, whole genome shotgun sequence.
ACCESSION   DAOFQD010000279 DAOFQD010000000
VERSION     DAOFQD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.284"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1684
                     /locus_tag="QBF46_004829"
     CDS             <1..1684
                     /locus_tag="QBF46_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6676903.1"
                     /translation="SQWDTTKTSLIDTLSINSGSTVNVADSTLISDSISLTGLSALNI
                     NEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKVTNDGILDVGNTAANAL
                     QVDNQNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTNDTTKSNYGYGLIAMNSD
                     GHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDNATGAGSIADYNGNE
                     LIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTN
                     IWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTK
                     IDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHF
                     NNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQ
                     LSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDS
                     IDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            1772..>2186
                     /locus_tag="QBF46_004830"
     CDS             1772..>2186
                     /locus_tag="QBF46_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6676904.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNS"
BASE COUNT          601 a          512 c          506 g          567 t
ORIGIN      
        1 ttcacagtgg gataccacca aaacttctct gattgatact ctgagcatca acagtggttc
       61 aactgttaat gttgctgatt caacgctgat ttctgactct atctctctga caggtctttc
      121 tgcgctgaac atcaacgaag atggtcatgt tgcaactgat tcactgactg tcgacaacag
      181 taccgtaacc atttctgatg aagtttctgc tggttgggct gtaggtgatg ctgctctgta
      241 tgccaacaac atcaaagtga ctaacgacgg tattctggat gtaggtaaca ctgcggcgaa
      301 tgctctgcag gttgataatc agaacctgac cagcactact gataccagtg gtaacattca
      361 cgctggtgtg ttcaacatcg aaagcaaccg cttcgtactt gatgcagacc tgaccaacga
      421 ccgtaccaac gatactacca agtcaaacta cggttatggc ttaatcgcaa tgaactctga
      481 tggtcacctg accattaacg gtaacggcga taacgacaac actgcttcta tcgaagctgg
      541 tcagaacgaa gttgataaca acggtgacca tgttgcagct gcaaccggta actacaaagt
      601 tcgtatcgac aacgcaactg gtgctggttc tatcgctgac tacaacggca acgagctgat
      661 ctacgtcaac gacaaaaaca gcaacgcgac cttctctgct gctaacaaag ctgacctggg
      721 tgcatacacc tatcaggctg aacagcgcgg taacaccgtt gttctgcaac agatggagct
      781 gaccgactac gctaacatgg cgctgagcat cccgtctgcg aacaccaata tctggaacct
      841 ggaacaagac accgttggta ctcgtctgac caactctcgt catggcctgg ctgataacgg
      901 cggcgcatgg gtaagctact tcggtggtaa cttcaacggc gacaacggca ccatcaacta
      961 tgatcaggat gttaacggca tcatggtcgg tgttgatacc aaaattgacg gtaacaacgc
     1021 taagtggatc gtcggtgcgg ctgcaggctt cgctaaaggt gacatgaatg atcgttctgg
     1081 tcaggtagat caagatagcc agactgccta catctactct tctgctcact tcgcgaacaa
     1141 cgtctttgtt gatggtagct tgagctactc tcacttcaac aacgacctgt ctgcaaccat
     1201 gagcaacggt acttacgttg acggtagcac caactccgac gcttggggct tcggcttgaa
     1261 agccggttac gacttcaaac tgggtgatgc tggttatgtg actccttacg gcagcatttc
     1321 tggtctgttc cagtctggtg atgactacca gctgagcaac gacatgaaag ttgacggtca
     1381 gtcttacgac agcatgcgtt atgaactggg tgtagatgca ggttatacct tcacctacag
     1441 cgaagaccag gctctgactc cgtacttcaa actggcttac gtctacgacg actctaacaa
     1501 cgataacgat gtgaacggtg attccatcga taacggtact gaagggtctg cggtacgtgt
     1561 tggtctgggt actcagttca gcttcaccaa gaacttcagc gcctataccg atgctaacta
     1621 cctcggtggt ggtgacgtag atcaagactg gtccgcgaac gtgggtgtta aatatacctg
     1681 gtaatattct tcactccgaa gaaattctgg taatttaatc taaataatgc ccgtcaagga
     1741 tttgacgggc attactgcaa aggacgcgca aatgttatct gtagttaaac ctcttcagga
     1801 atttggtaag ctcgataaat gtttgtccag atacggtacg cgcttcgagt ttaataatga
     1861 aaagcaagtt atattttcca gtgatgtcaa taacgaagat actttcgtta ttttagaggg
     1921 agttatctct ctgcgtagag aagaaaacgt acttatcggt attacccagg ctccttatat
     1981 tatggggctg gctgatggtt taatgaaaaa cgatatacca tacaaattaa tatcagaagg
     2041 aaattgtacg ggatatcatc taccagccaa acaaaccatt acgcttattg aacaaaatca
     2101 actctggcga gacgcttttt actggttagc ctggcaaaat agaattctgg aattacgtga
     2161 cgtgcagctc attgggcata attcct
//
LOCUS       DAOFQD010000280         2178 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.285, whole genome shotgun sequence.
ACCESSION   DAOFQD010000280 DAOFQD010000000
VERSION     DAOFQD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.285"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            85..348
                     /locus_tag="QBF46_004831"
     CDS             85..348
                     /locus_tag="QBF46_004831"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6676905.1"
                     /translation="MRPQRRTYSKSFKAQVIQECSEPGASIANIALGYSLNANLVHKW
                     IRLLTQKTTAIQPAFIPLPSQMLGAGSHAQARCCDHEFDPVGP"
     gene            293..457
                     /locus_tag="QBF46_004832"
                     /pseudo
     CDS             293..457
                     /locus_tag="QBF46_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            675..1973
                     /locus_tag="QBF46_004833"
     CDS             675..1973
                     /locus_tag="QBF46_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDQ6676906.1"
                     /translation="MDDKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAELT
                     HHLGHDKNQPKSGTNSRNGYSTKTVTTTDGPMELRTPRDRDGSFEPQLVKKNQTRITG
                     MDNQILALYARGMTTREITSVFKEMYDAGVSPALISKVTDAAIDQVVEWQNRPLDAIY
                     PIVYLECIVLKVRQDSRVINRAVFLALGINLDGQKELPGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPEAINAVYPETQIQLCIVHMARNSLRFLAWKDYKAVTRD
                     LKQVYRAATEEGVLQALDAFGKEWDARYPQISRSWRANWPNVATFFVYSEAIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPIDESVKKVMRLAIQSASQKWTMPLQDWRIAMSRFII
                     EFGDCCGQLKLATALEFFQYRFSDSFGGNPPLYSCGLSAL"
     gene            complement(1871..>2178)
                     /locus_tag="QBF46_004834"
     CDS             complement(1871..>2178)
                     /locus_tag="QBF46_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6676907.1"
                     /translation="GSHYTSRQFRQLLWRYRIRQSMSRRGNCWDNSLMERFFRSLKNE
                     WVPATGYVSFSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNAVASFS"
BASE COUNT          538 a          591 c          549 g          500 t
ORIGIN      
        1 caacgtagtt caacagtatc cgtccggcca acacaccttc ccgacaccgc cgccgaaggc
       61 catggcccat caagcggatt ttccatgcgc cctcagcgcc gtacctacag caaatctttc
      121 aaggcccagg tcattcagga gtgttctgaa cccggtgcct ccatcgcaaa catcgccttg
      181 ggctacagcc tcaatgccaa cctcgtccac aaatggattc gcctgcttac ccagaaaacc
      241 acggctatcc agccagcgtt catcccgttg ccttctcaaa tgctcggcgc gggttcgcat
      301 gctcaagcgc gctgctgcga tcatgagttt gatccagtcg gcccgtaata atgggcacga
      361 tccgtatgcc tatctcaagg atgtgctgag acggttgccg acgcagcgag ctagtgagat
      421 tgcagagctg ctgccgcata ggtggcatcc ggcatagtcg cgcaagatgg tttgcccgca
      481 tgcttacgtt caacagctca agcaccgtcc gcccttcgcg ggatcgttgc cgccagagcc
      541 acacgccgaa cttttccacc ttgtcgtcgc cggcctgcgg tcagtcaggc aagcccaggt
      601 gcgagcctgt acatatattt gtgtaattgc ctgattttga tatgttcaat tcaacatcaa
      661 atgaaggtta aattatggac gacaaacaat tgcaggctct ggctaacgaa ctggccaaaa
      721 atctcaaaac ccctgaagat ctcagtcagt tcgatcgcct gctcaagaaa atcagtgttg
      781 aggctgctct caacgctgaa ctgactcacc atctgggaca tgataaaaat cagcccaaat
      841 cgggaaccaa ctcccgtaat ggctattcca cgaaaaccgt caccactacc gacgggccga
      901 tggaattacg tactccacgt gatcgtgatg gttccttcga accacaactg gtgaagaaaa
      961 atcagacccg cattaccggc atggacaacc agatcctggc gctgtatgcc agagggatga
     1021 ctacccgcga aattacctca gtcttcaaag aaatgtacga tgcaggcgtc tctccggcgc
     1081 tgatatcgaa agtcaccgat gccgcaatcg accaggttgt agaatggcaa aaccgtcctc
     1141 tggatgcgat ttatcccatt gtttatcttg aatgtattgt cctgaaagta cggcaggaca
     1201 gccgggttat caacagagcg gtgttcctgg ctctgggtat taaccttgat ggtcagaaag
     1261 agctgccagg tatgtggctg gccgaaaatg aaggtgcaaa gttctggctg aatgtactga
     1321 cggaactgaa aaatcgcggg ctgaacgata tccttatcgc ctgtgtggac ggcctgaaag
     1381 gcttcccgga agcgattaat gccgtctacc cggagacgca gatccagttg tgcatagtac
     1441 acatggcacg caacagcctg cggttcttgg cgtggaaaga ctataaagcg gtaacccgtg
     1501 acctgaaaca ggtttaccgc gcagccacgg aagaaggcgt gttgcaggcg ctggatgcgt
     1561 tcgggaagga atgggatgca cggtacccgc agataagtcg cagctggcgg gcaaactggc
     1621 cgaatgttgc caccttcttc gtgtattcgg aggcgatccg caaggttatc tacaccacta
     1681 acgcaatcga atcgctgaat agcgtcatca gacatgcgat aaagaaacgg aaagtgttcc
     1741 cgatagatga atcggtgaaa aaagtgatga ggctggctat ccagtcagcc tcacagaaat
     1801 ggacgatgcc acttcaggac tggcggatag ccatgagtcg ctttattatc gagttcggtg
     1861 actgttgtgg tcaactaaaa ctggccaccg cgttagagtt tttccagtat cggttttctg
     1921 attcgtttgg tggtaaccca ccattatatt cgtgcggtct tagtgcgctg taatatccaa
     1981 cgatatagtc cgttattgcg tgagctgcat cgctgaagct tacatagccc gtcgctggca
     2041 cccattcgtt cttcagactc ctgaagaagc gctccattag gctgttatcc cagcagtttc
     2101 cacgccgact catactctgc ctgatccggt atcgccacag taactgccgg aactgcctgc
     2161 tcgtataatg actgcctt
//
LOCUS       DAOFQD010000281         2140 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.286, whole genome shotgun sequence.
ACCESSION   DAOFQD010000281 DAOFQD010000000
VERSION     DAOFQD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.286"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(448..777)
                     /locus_tag="QBF46_004835"
     CDS             complement(448..777)
                     /locus_tag="QBF46_004835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676908.1"
                     /translation="MSRYGDSARNVFEIYYETGLTLPLYLSRTTWSPTTLVVIKSIGG
                     LTSGKLSGMGRYPYFNEASVICDIYGPQPAFNVDCPGRGSYQWKLSPLRPSDFPSIDA
                     SRSQIEI"
     gene            complement(777..1808)
                     /locus_tag="QBF46_004836"
                     /pseudo
     CDS             complement(777..1808)
                     /locus_tag="QBF46_004836"
                     /inference="COORDINATES: protein motif:HMM:TIGR01629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1801..2076)
                     /locus_tag="QBF46_004837"
     CDS             complement(1801..2076)
                     /locus_tag="QBF46_004837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676909.1"
                     /translation="MPSKRPRATFLLTPELMNAIRSLSDVSGMTMSAIVVQFLEPSIP
                     VMKQLEEVVLEAKKGAAGNGRAIVGSILEGANKTLAELNREFGELDD"
BASE COUNT          707 a          496 c          478 g          459 t
ORIGIN      
        1 aaaaaaaata agcaagaaat gggggtagtg tcttagatgt gagacaaatg tgcagtatta
       61 cagacactgc acatcgagat cggtaatggc gaggatgcga atcagaacgg cgtgtgcctc
      121 ctcgcagagc tgcgggggca atccccgtcc cgattcgtga tcgcacgaca aaccctatca
      181 ccctggacag agttgcaaat ctacaaataa ggcataccga gcaaaacgaa aataccccca
      241 cggcatcacc tgtagggcag ggcttacgca cctaccccac aggcaacgcc ttgaaggggg
      301 taaaaagcgg ctttacaggg cgatggctca ccatgtgaac catcacccat aactaggatg
      361 aatagggagt gtcgtacaac cactaaatcg gaaccctgaa gggatccccc gatttagaag
      421 ttgacgagtc ggcggcggcg aacgctgtca gatttcaatc tgagagcgcg aggcgtctat
      481 agaggggaag tcggaagggc gcagagggct tagcttccac tgataagagc cgcgacccgg
      541 gcaatcaacg ttaaaagcag gctgagggcc ataaatatca caaataacag aggcctcatt
      601 aaagtatgga taacgaccca tgccacttaa cttgccagag gtaaggccac caatagactt
      661 aatgacgact aaagttgtag gagaccaagt ggtacgagaa agataaagag gcaaagttag
      721 gcctgtctca taataaatct caaaaacatt gcgagcagaa tcaccatatc tagacattaa
      781 ataactccat caaaatacaa atcagtgcca atagcccaat caggcacacc cataggctta
      841 gcctctatga tgcgtatgaa gggaacgaca ttatcaggct gcttgtattg gacagtggcg
      901 atatcaatac ccaaggcatc cataagagcc ttacggtagc gatagaagct agggcgagag
      961 tacatagacc taatatcgtg gccacacttc caaagctcat agacagcctt ataagcagga
     1021 gataaaccat cagtcttttc agaagtgata gcagtaatgt cagccatgtt aatacgtcct
     1081 ataaaatcat ttactatttg cagaggaagc aagtcatccc atgccgaacc ggaggaaaga
     1141 ccgcgatgat taagctcaag cgaacgtaaa acaacctcac aacgcaaacc acgattagcg
     1201 tagtcaacga gcgcaggaag ctgtaagaaa gctgcgtgag agtgcttttg gaactcctca
     1261 cccttagcat agaatttcac tgagctacgg cgcgacgatt taccaaagta taacgtagat
     1321 cctttagtga cctgaccacg cccacgatga gaaagagtgg cactcatctc ggcctgacga
     1381 atccaagaca gtgcgttagc agtattgcca acatcaaaca taacagtaca gtcaacacgg
     1441 tttaacttga aatccccctt ttcccaagaa cgaacatcaa aatcagttgg attaagacaa
     1501 agctcaggaa tagaagttat tttctttaaa acagtagcaa gcaatggaat taaattatcc
     1561 gttccaaaaa ggttatggcc ttgaaggaat ttaactggat tcccatcaaa atagatatga
     1621 gtcccgttac caaagtcatt aacagaatgg gttttaagtc ttaatgatga atcatggcta
     1681 ccaacaacag aaatagcggt aagagattcc cactcaacct cacctgatga atttatttta
     1741 gtgatcctgc ctgaatctaa tggcttttca tgaaagcaag gaactacagc agttatccaa
     1801 tcaatcatct aattccccaa actctctgtt aagctcggca agagttttat tggcaccctc
     1861 aagaatagaa ccaacaatag cccgaccgtt accagcagca cctttcttag cctccaagac
     1921 aacctcctct agctgcttca taacagggat tgatggctct aaaaattgaa caacaatcgc
     1981 cgacatggtc atgccagata catcagaaag agaacgaatt gcgttcatca gttcaggggt
     2041 cagaaggaaa gttgctcgcg ggcgcttaga aggcaaaatg gactcctttc ttaaatgtga
     2101 gacaaagtgt gcagtggggt aagacccatt gtaaccctag
//
LOCUS       DAOFQD010000282         2130 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.287, whole genome shotgun sequence.
ACCESSION   DAOFQD010000282 DAOFQD010000000
VERSION     DAOFQD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.287"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            751..900
                     /locus_tag="QBF46_004838"
     CDS             751..900
                     /locus_tag="QBF46_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676910.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPV
                     GREAL"
     gene            complement(1711..2034)
                     /locus_tag="QBF46_004839"
     CDS             complement(1711..2034)
                     /locus_tag="QBF46_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676911.1"
                     /translation="MKKLVAIGLGVLILSGCATQKQMTPMGGSKADGTVKMGYTFGMF
                     EKPVVDLNSAKDLAGQKCKTWGYTGAEAFGGQTSTCAQVGAYGCEMTNVSIEYQCTGG
                     KASEN"
BASE COUNT          493 a          534 c          490 g          613 t
ORIGIN      
        1 aaaaaaatca aagaaagcct aatgcccccg tggtgggtat ataaaaatac ggggagcgga
       61 gcgaccttcg tctagctctt gttttgtttt tcttgccgcg caaccggctt gattgtgtgg
      121 gtgttgtgtc cgacgtctct cacatcaccg cgtactgctg cctttttcgc tcattttatc
      181 aggtgccagt tatcgtactg cgagacgggg tagagcttaa ctctttcgcg gttaagttac
      241 gtttccacca attgcctgtt tatcctgccg gagtccgggg cgtagacctg agcgtcttag
      301 gtgcagatgt ggatttaatg tcgttgaatg gtattaagct ggtagtgaca aggctagcca
      361 gccctgctag gatttgattt ctgacgtcta ttcctggcag gcatctgcga agaattagag
      421 taccgaagcc ctgtgattct gtcacggggc ttttgtacat ttagcgcccg agaatcaaaa
      481 acatcccttc agaacttatg taacgcccgc cgcagtctca cggcaggagc gcagcgaccg
      541 agtgagcgag gaggcgacat ttcatcctta cctgcatgtt gcacttacgc cccgcagcta
      601 catttttccc ctgccacatg aagcacttcc ctgaattccc acttcgcttt accatattaa
      661 gccagtatac actccgctag cgctgcgtga ctggttcagg gctgcgcccc gaaacccgct
      721 aacaacctct ccgctactgg atatcacccc atgccatctg aatatgatga acaactggat
      781 tcgactgata cgcagcttcc cggcgaatat gacctttttg atttaatggc gcaggttact
      841 gaagagaacc tgcacgcccc ggtggatttt gggccgcctg tcggccggga agcgttatag
      901 ctttttcgct tacccgcgta cgtgtaatac gccggtatta catcttattt ctttattttc
      961 atgtgattac aggtggttgt aatacgttgc gtattacata aataaacggg aacttcagtt
     1021 tttacctttt aattcatgca attgcgatga aaatcgcggt tgcgtatggt gtatggccat
     1081 ttaagggata aagaatcatc tccttacctg gctgagcttt gcctattctg aggtgatctg
     1141 tgaaaaaaag cgtccataac tagctcgttc atacttgtat gaacagctta ttcctcctga
     1201 ctactaataa actacagcgt cactactgat ttactactga tttactacaa accggagggg
     1261 aggactacag aaacactact gatttactac aaatcggagg aggatgacta cttatctact
     1321 actgatttac tactgaatta ctactgaatt acgtcagaac agttctctga accattcgta
     1381 catccgcccc tcttcctggg cctcctgcac gtgctgaaaa tggtttttcc ggctgtgccg
     1441 gcaacgcctc acggccacgc aataaattgc gcatccgttc accgttatcg gggtggttcg
     1501 tggtggcttt cagggcgttc atcggatttt agcggctaaa atcccggcca ggtatgtccg
     1561 tgcatagcca tgcagtcgcg ccagaatcat caggggcgtt ttttctcgat atgggcacag
     1621 aaaggtgtgt gtttcaggcc ggaggcctca gaaacgcgct gagggcctct gcggcggtgc
     1681 acgcaacgga tgttatggta aatgttgaac ttagttttct gaagctttcc caccggtgca
     1741 ttggtactcg attgaaacgt tggtcatctc acaaccataa gccccgacct gtgcgcaggt
     1801 ggatgtttgg ccgccaaatg cctcagcccc agtgtatccc catgttttac atttttgtcc
     1861 agccagatct ttagctgagt tcagatcaac aacgggtttt tcaaacatcc caaaggtgta
     1921 acccattttc actgtaccat cagctttact cccccccatc ggggtcattt gtttttgcgt
     1981 tgcgcagcct gaaagaatta aaacgcctaa accgatcgct actagttttt tcatttttta
     2041 tttcccggat gattgttcgg aattatctta acgggaagtt tacacaaggc aacaccctct
     2101 gtaatcctct gattatttga ggtatcccat
//
LOCUS       DAOFQD010000283         2120 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.288, whole genome shotgun sequence.
ACCESSION   DAOFQD010000283 DAOFQD010000000
VERSION     DAOFQD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.288"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1925
                     /locus_tag="QBF46_004840"
     CDS             <1..1925
                     /locus_tag="QBF46_004840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6676912.1"
                     /translation="FELASGANIETHGKNASALFISNDDGEVNIKTASKIIASGAGSD
                     GIEIDSALRGANIINTGEISASDIGINFNTGVQGNVFNAGTISSDNNIAVHFADEINT
                     LTLTSGSQIKGSVLGGAFDDSLILQGTNSEDLSKFNDFNKLVMEGDEWTLNDNGIFSD
                     SYTQKSGVVNLVGTLVTPQVNVEGGTLNLWKSQTWDTLINGGKISLNTTGNVGNTLNV
                     KGDYNGSNGVLVFNTALGSDHSLSDKLVICGNAMGTTSVVVNNAGGTGAHTIDGIELI
                     QVNGTSTDTAFTQAGRIVAGAYDYKLVKGNASGTNTQSWFLTNPDPYNPDNPTIRPEA
                     GSYMANIDTARKIFNSRLEDRERRSENSSMWLRQQGTRTTFYDTSGQSYSATNTYVVQ
                     GGGELFSANFSDLDRLGIGMMLAYGNSSNNTGSNRTGYHSKGSVDGYSAGLYATWYQD
                     AGTLNGIYIDTWLQYSWLNGMVKGEQLSNENYKIDGLSASVETGYRKLIYQGANGNVF
                     LIPQGQFIFSGVEADDVKEFNGTHVTTSGNNNIQSRLGVKLSREGTSKIDKGKDKLFT
                     VYAEANWLNNSRQVGAEMNGVSIKQAGNKNVAELKLGTEGKFNKNMNLWTNVAQQIGA
                     KGYSDTTMTIGFKYSF"
BASE COUNT          684 a          364 c          502 g          570 t
ORIGIN      
        1 ttttcgaact ggcatctggt gcaaatatag agactcatgg aaaaaacgca agtgcattat
       61 tcattagcaa tgatgacgga gaagttaaca ttaaaaccgc cagcaaaatt attgcatctg
      121 gcgcagggag cgatggtatt gagatagatt ccgcactgcg cggagcgaat atcatcaata
      181 ccggggaaat ttcagcctca gatattggaa taaattttaa cactggagtg caaggcaatg
      241 ttttcaatgc tggaacgatt agcagcgata ataatattgc cgtacatttt gcggatgaaa
      301 taaatacgct gactcttacc tctgggtctc agattaaagg gagcgtctta ggtggagctt
      361 tcgacgactc tttgatttta cagggtacaa acagtgaaga tctctccaaa tttaatgatt
      421 ttaacaagct ggtaatggag ggggatgaat ggacgctgaa tgataatggc atatttagcg
      481 atagctatac acaaaaatcc ggggttgtga atttagttgg aacattagtt acccctcagg
      541 ttaatgttga aggtggcacc cttaacttat ggaagagtca gacatgggat acgctcatta
      601 atggtggaaa aatatcttta aacactacag gaaatgttgg taatacgctg aatgtaaaag
      661 gtgattataa tggtagcaat ggtgttctgg tatttaatac agctttagga agtgatcatt
      721 ccctcagcga taagttagtt atatgtggta atgctatggg tacaacctcc gtagttgtga
      781 ataatgcagg tggaactggg gcacatacca ttgatggtat tgaactcatc caggtaaatg
      841 gtacgtcaac tgatactgca tttactcagg caggacgtat agtcgcggga gcatatgact
      901 ataaactcgt aaaaggtaat gcatcaggaa ccaatactca aagctggttc ctgacaaatc
      961 ctgatcctta caaccctgac aacccaacca ttcgtccaga agctggaagt tatatggcca
     1021 acatagatac ggccagaaag atatttaatt cacgcctgga ggaccgcgaa agacggtcag
     1081 aaaattcaag tatgtggtta cgtcagcaag gaacaagaac caccttttat gacacttctg
     1141 gacagtccta cagcgctaca aacacatatg tggtacaggg tggcggagaa ttattcagtg
     1201 caaacttcag cgatttggat cgccttggga ttggcatgat gctggcctat ggtaactcaa
     1261 gtaacaatac tggctctaat cgtacagggt atcattccaa aggatccgta gatggttaca
     1321 gtgccggttt atacgccacc tggtatcagg atgctggcac tttgaatgga atatatattg
     1381 atacctggtt acagtacagc tggttaaatg ggatggtaaa gggagaacaa ttatctaatg
     1441 aaaactacaa gatagatggt ctaagtgctt cagtcgagac tggatatcgc aaattaatct
     1501 atcagggagc taacggaaat gtttttctca ttccgcaagg acagtttatt ttcagcggtg
     1561 ttgaggcaga tgatgtgaag gaatttaatg gaactcatgt aaccacttca ggcaataaca
     1621 atatacagag tcgattaggt gtaaagttat ctcgtgaagg tacaagtaag atcgacaaag
     1681 gaaaagataa gctgtttact gtttatgcag aagcgaactg gctgaacaac agccggcagg
     1741 tgggggctga aatgaatggc gtcagtatta aacaagcggg taacaaaaat gttgctgagc
     1801 ttaaactggg cactgaaggg aaatttaata agaatatgaa tttatggacc aacgttgctc
     1861 aacaaatagg tgctaaaggc tacagtgata caactatgac aataggtttt aaatatagtt
     1921 tttaatcagc gacaggggca tggtggctcg tagcatccac catgccaatt ctgccaggct
     1981 gtagatacac gctaattttc agtttctggg tatttctcac ctgagataat tgtataaaag
     2041 tgaaatagtg attatgaatc taaaaatcag tgttctttta gaaaacaggt taaatacagg
     2101 ctcagcagat aaattaattg
//
LOCUS       DAOFQD010000284         2030 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.289, whole genome shotgun sequence.
ACCESSION   DAOFQD010000284 DAOFQD010000000
VERSION     DAOFQD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.289"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            137..1270
                     /gene="ompN"
                     /locus_tag="QBF46_004841"
     CDS             137..1270
                     /gene="ompN"
                     /locus_tag="QBF46_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDQ6676913.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1411..1845
                     /gene="uspF"
                     /locus_tag="QBF46_004842"
     CDS             1411..1845
                     /gene="uspF"
                     /locus_tag="QBF46_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDQ6676914.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          550 a          461 c          460 g          559 t
ORIGIN      
        1 gaggaattat ttgattaagg ttttacttaa ggcgtaacaa atgatattta tcggctaact
       61 gaacttctcc tttaggatgt tttcactccc cgaaatgggg ataaaaggca aataaaataa
      121 ctaaaggatt tattcaatga aaagcaaagt actggcactt ttaattcctg ccctgctcgc
      181 cgcaggtgct gcacatgcag ccgaagttta taataaagac ggcaacaaat tagatctgta
      241 tggcaaagtt gatggcctgc attatttttc tgataattcg gcgaaagatg gcgaccagag
      301 ctatgcgcgt ctgggtttta aaggcgaaac ccaaattaac gatcaactca ctggctacgg
      361 tcaatgggaa tacaatattc aggcaaacaa cactgaatct tcaaaaaacc agtcatggac
      421 ccgtctggca tttgccgggc tgaaatttgc agattacggt tctttcgatt acggacgtaa
      481 ttatggcgta atgtacgaca tcgaaggctg gaccgatatg ctgcctgaat ttggcggtga
      541 ctcttatacc aatgcagaca actttatgac tggtcgagcc aatggcgtcg cgacttatcg
      601 taatactgat ttcttcggtc tggtaaatgg tctgaacttc gcggtgcagt atcaaggtaa
      661 caacgaaggt gctagtaatg gtcaggaagg caccaacaac ggacgtgatg ttcgccatga
      721 aaacggtgac ggctggggtc tttccacaac atatgattta ggcatgggct ttagcgctgg
      781 tgcggcatac acctcttctg accgcaccaa tgatcaggtt aaccatactg cggcgggtgg
      841 tgataaagca gacgcgtgga ctgctgggct aaaatacgat gctaacaata tttacctggc
      901 aaccatgtat tcagaaacgc gtaatatgac cccgtttggc gacagcgatt atgctgttgc
      961 aaacaaaacc cagaactttg aagtcactgc acagtaccag tttgattttg gtctgcgtcc
     1021 ggcagtctct ttcctgatgt ctaaaggccg tgacctgcac gctgccggtg gtgcagacaa
     1081 cccggcaggt gttgatgata aagatctggt taaatacgcc gatgttggcg cgacttacta
     1141 tttcaataaa aacatgtcca cttatgttga ctataaaatc aacctgttgg atgaagatga
     1201 cagtttctac gctgccaatg gcatctctac cgatgatatt gtcgctttag gtctggttta
     1261 tcagttctaa atcctcctgc ccgctgttat ggcgggcttt ttctgcttat tcttcctcct
     1321 ttgatctaaa ttaaaaatgt gaactccgtc attacacaaa aagtgtcatc tggcgttaca
     1381 ctttatgcgg atactaaaac aggaggtttt atgaacagaa cgattcttgt ccctatcgat
     1441 atttccgatt cagaattaac tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt
     1501 gatgatgcag aggttcattt cctgacggta ataccttcac tgccctacta tgcctctctg
     1561 ggtttagcgt attccgcaga attaccggca atggatgacc tgaaagcgga agccaaatcg
     1621 caactggaag agatcattaa aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt
     1681 gaggaaggct cgcccaaaga ccgcattctg gaaatggcga agaagatccc cgctcatatg
     1741 atcatcattg cttcccatcg accggatatc accacttatc tgctcggttc caacgccgca
     1801 gctgtcgtgc gtcacgcaga gtgctccgtg ctggttgtgc gctgacacta acgcccgcac
     1861 attgctgcgg gctttttgat tcatttcgca aatgtgctga cattttcccc tctaatccgt
     1921 accatacacg ccactgtttt tatatcagat ttcttatgct gggcattccg gcatgacgca
     1981 tactcttctg atgccatata acgaattgag tcgcttttaa atgtcgcaaa
//
LOCUS       DAOFQD010000285         1934 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.291, whole genome shotgun sequence.
ACCESSION   DAOFQD010000285 DAOFQD010000000
VERSION     DAOFQD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.291"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..850
                     /locus_tag="QBF46_004843"
     CDS             <1..850
                     /locus_tag="QBF46_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDQ6676915.1"
                     /translation="KSESTFSYFEATRGYIEKYGKPMILYSDKASVFRVNNKHATTGP
                     GETQFARAMRCLNITPLCAETSQAKGRVERAHLTLQDRLVKELRLKGISTIEAANAFA
                     EEYMADYNRRFAKAPLHDFNAHRPLALDDDLDAEFTWREPRRVSKSLTVQYDKMLYLI
                     EDSEYSRRAIGKYIDVWHYPDGHKELRLNGVLLPYSTYDRLSEVDPVAIVDNKRLGHV
                     LDVARQVQKKRDNNRSQSLPSGDEPSRRRHAPGINKSQRSLNEDDLLEAIIKLQGSSE
                     AIFGKR"
     gene            1113..1919
                     /locus_tag="QBF46_004844"
     CDS             1113..1919
                     /locus_tag="QBF46_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HDQ6676916.1"
                     /translation="MYMTGQEINDKKKEYLELLDREENEQIYQTYLEENTMFIPREFE
                     QNHGIHFSTVFRKLPLSSDYKPDFVYLSKSSDNWNVVLVEIEKPSSKYFKNNSTTFHA
                     DFNLALQQMNTWRAWFDDESNRNHFKNNILQGFIEPAHMGRNPFNFKYVLVHGRRSEY
                     ENNTQKTALIRGQQRSDFSIISFDSLAENIEKKYKLYVGVKKNSHYELISKEFVDEGI
                     FSWMNIDFLAITQSIYDDAIAKSDSWHHYIIKENGTVKTMDYALPRIKII"
BASE COUNT          658 a          386 c          409 g          481 t
ORIGIN      
        1 taaatctgag tccaccttta gctattttga agcgacccgt ggctatatcg agaaatacgg
       61 taagcccatg atcctttaca gcgataaagc cagcgtattc agagtcaata ataagcacgc
      121 taccactggt cccggtgaaa cccagtttgc ccgagccatg cgctgcctca acattacccc
      181 gctatgtgcg gagaccagcc aggcaaaggg ccgtgtggaa cgcgcccacc tgacacttca
      241 ggaccggctg gttaaggagc tcaggcttaa agggatcagt acgatagaag ccgcgaatgc
      301 atttgctgag gaatacatgg cggattataa ccggcgcttc gccaaagccc ccctgcatga
      361 ttttaacgca catcggccgc tggcattaga cgatgacctg gatgcggaat ttacctggcg
      421 agagccccgt cgtgtatcaa aaagtctgac cgtacaatat gacaaaatgc tctatctgat
      481 tgaagacagc gaatacagta gaagggctat cggcaagtat attgatgtct ggcactatcc
      541 tgacggacat aaggaactgc ggttgaatgg cgtgctactg ccctactcca cctacgaccg
      601 attgtctgaa gtcgatcctg tcgcgattgt tgataacaaa agactgggcc atgtattgga
      661 tgttgcccgc caggtccaga aaaaaaggga caataatcgc tcccagtcgc taccttcagg
      721 agatgagcct tcccgcagac gacatgctcc aggcatcaac aaatcccagc gctcgctgaa
      781 cgaagatgac ctgcttgaag ccataataaa gcttcaagga agctctgagg ccatctttgg
      841 taaaaggtga acttgtcgga aacctcgcac tcactgacca gcagcgctgt gttgcagtta
      901 atgcaatcaa agcctgaaaa aaattctgag aaatgcaaat aacttcaatg atattatata
      961 caaagagaaa cgtcgggtag ccggggtaaa gtccgggtgt cacagaaccc cgaaccgctt
     1021 cgcggtgact tcttaccaca aaccaagtct atgtaaaatt tattgcgtag atatgaatct
     1081 aatcagagcc ttaaatttaa agatggtaaa ttatgtatat gactggtcaa gaaataaacg
     1141 ataaaaagaa agagtatctt gaattactag atcgagaaga gaatgaacag atttatcaaa
     1201 cataccttga agaaaataca atgttcatac caagagaatt cgagcaaaac catggaattc
     1261 acttcagtac ggtatttaga aagttgcctc tatcaagcga ttataaacct gatttcgtat
     1321 atttatcaaa aagctcggat aactggaatg tagtattagt tgaaattgaa aaaccatcgt
     1381 cgaaatattt taaaaacaac tctacaactt ttcatgcaga cttcaatctt gctttgcagc
     1441 aaatgaacac atggagggct tggtttgatg atgaatcaaa cagaaatcac tttaaaaaca
     1501 atatattgca gggatttata gaaccggcac atatggggag aaatccattt aatttcaagt
     1561 atgtccttgt tcatggtcgt agatcagaat atgaaaataa tacacagaaa actgcactaa
     1621 tcagagggca acagcgaagt gacttctcaa taatttcatt tgatagtttg gctgaaaata
     1681 tagagaaaaa atataagtta tatgttggag tgaaaaaaaa tagtcactat gaattaatat
     1741 caaaagaatt tgttgatgaa gggatatttt catggatgaa catagatttt ttagcaatta
     1801 cacaaagtat atatgatgat gcaatcgcaa aatcagatag ttggcatcac tatataatta
     1861 aagaaaatgg aaccgtcaaa acaatggatt atgctctccc tcgaattaaa ataatttgag
     1921 agtagtaaaa aaaa
//
LOCUS       DAOFQD010000286         1920 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.292, whole genome shotgun sequence.
ACCESSION   DAOFQD010000286 DAOFQD010000000
VERSION     DAOFQD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.292"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            257..553
                     /locus_tag="QBF46_004845"
     CDS             257..553
                     /locus_tag="QBF46_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6676917.1"
                     /translation="MDTIFPVRLLSQLRPLLIGFRKSKGLTQRELSAMLGVTQQTYAR
                     LEANPSKASFERLYKVLNILGVEISLSSAPLSTYTKPTNFVEKEFDSPARREEW"
     gene            complement(1036..1371)
                     /locus_tag="QBF46_004846"
     CDS             complement(1036..1371)
                     /locus_tag="QBF46_004846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676918.1"
                     /translation="MIVPLIFELLSITNTDQRRSYVLAGGESANRVGLFWIRKTGIME
                     GIPEHYLIEGTLNRPGVVEYLRHIIFIWDSISSEISEMFTFGTKKMLLNFTNVLRTAP
                     DLNDDTQEK"
     gene            complement(1372..>1920)
                     /locus_tag="QBF46_004847"
     CDS             complement(1372..>1920)
                     /locus_tag="QBF46_004847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676919.1"
                     /translation="HAISITYKLIRSLYDDAAAFVDYVHSGKDDEAYDMWFNGQTNFT
                     TLRDEWDKTNFTKNIFLAGLTWVFFHELGHLTQGHLFIRNRYSSDINVMSEIHLNNLS
                     SDNNDASAIWHATELAADYFSTCMCVVEIVRQLAINENCELAICFLTCGISLMLHRFQ
                     GEKFYEVQTEPVGTHPRPFVRL"
BASE COUNT          598 a          412 c          353 g          557 t
ORIGIN      
        1 agcagagtcc gccctctccc ttcataccct gatcagcctc attacaatga ggttaacagc
       61 gtacaccact atatgctagt ccttaataaa tataatccca gccagagcga gatgcaacct
      121 ttaaaattca agcagtacta gttaaacaac gagttcatcc ttacaccttt taaactgtaa
      181 agtaataaaa caccttacag gctgtttttt atgaggattg aatttattat gaagaaatcg
      241 acatggccac gtacttatgg atactatttt tcccgttaga cttctaagcc agttacgtcc
      301 acttttaatt ggttttcgta aatcaaaagg attaactcaa agagaattat ctgccatgtt
      361 gggtgtaact cagcagactt atgctcgcct tgaggcaaat ccttcaaaag caagttttga
      421 gcgtctatac aaagtgttaa atattttagg ggtggagatt tcgctcagtt ctgcacctct
      481 ttcaacttat acaaagccta cgaattttgt agaaaaggag tttgattcac cggcaaggcg
      541 tgaggaatgg tgataggagc aagcctattt gataggtctg gacatcaggc accgcctcta
      601 agcatttgat ctgtttcccc attgatcaac tcagactgtt tagccacgcc cgattctggc
      661 tcggtgcgga taaattgacc aatttaaatg tccgctgtga gcgatgagcg gacgttaatc
      721 tggggttgta cgccggaacc gccgaaattt ccgtgacgca taatttatgc ggttgtctgc
      781 ctgtacgtaa gaccgccgtc aaccggacag taaagaaatg gccgtttgga acgaaaaatc
      841 actgaccgca gcgtctcaca tagctggtac ggataaattc tcactcacgg caccataaac
      901 gtacaacgta caacgtacac gtcagcacat gccaaacatg cttcaaaatt cgactaattc
      961 actgcgccag aaatttcggc ggttccggca cagaacccct atattatgcg aattgttaat
     1021 taacactgag cagcattatt tttcctgtgt atcatcattc aaatctggtg cagtccgaag
     1081 tacattcgtg aagttgagaa gcattttttt tgtgccaaaa gtgaacatct cagagatttc
     1141 agatgaaatg ctatcccaaa taaaaataat atgcctcaaa tactcaacaa ctccaggccg
     1201 gttcaatgta ccttcgataa ggtaatgttc aggaataccc tccattattc cagtctttct
     1261 tatccagaac agtccaaccc tgttggccga ttcaccacct gcaagcacat aactacgacg
     1321 ttgatctgtg tttgtaatag aaagtaattc aaaaattaaa ggtacgatca tttaaagcct
     1381 tacgaatggc ctaggatgtg taccaactgg ttctgtttga acctcataaa atttttcgcc
     1441 ttgaaaacga tgtaacatta atgatatacc acatgtcaaa aagcaaattg ccaattcgca
     1501 attttcatta atggcaagtt gtcgaacaat ttctactaca cacatgcatg tggaaaaata
     1561 atcggctgca agttccgttg cgtgccaaat ggcagatgca tcgttattgt ctgatgataa
     1621 attatttaag tgaatttcag acattacatt aatatcggaa ctatacctgt tccttataaa
     1681 taaatggccc tgagttaagt gtcctaactc atgaaaaaaa acccatgtca aacctgctag
     1741 aaaaatgttt ttagtaaaat tagttttatc ccactcatct cttaaagtgg taaagttggt
     1801 ttgcccgtta aaccacatat cataagcttc atcatcttta cccgagtgaa cataatctac
     1861 aaaagcagca gcatcatcat ataatgatcg gattaattta tatgtgattg atatagcatg
//
LOCUS       DAOFQD010000287         1914 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.293, whole genome shotgun sequence.
ACCESSION   DAOFQD010000287 DAOFQD010000000
VERSION     DAOFQD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.293"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..174
                     /locus_tag="QBF46_004848"
     CDS             <1..174
                     /locus_tag="QBF46_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676920.1"
                     /translation="YIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQAVFQKIV
                     SFIEKFKGVGGKI"
     gene            complement(441..947)
                     /locus_tag="QBF46_004849"
     CDS             complement(441..947)
                     /locus_tag="QBF46_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HDQ6676921.1"
                     /translation="MSIYNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     EHWNEFNLACDTDAPITPVIDMVRQLSGIYTLWFVTGRCEIARDKTRAWLRKHVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRSHGVLTLMVREY
                     ENALLHQQ"
     gene            complement(1021..>1914)
                     /locus_tag="QBF46_004850"
     CDS             complement(1021..>1914)
                     /locus_tag="QBF46_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676922.1"
                     /translation="NAERISIELETKLNEMTAMVELRNSQISTLKSQYKEIMKLDPFN
                     LEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALVNENAKFA
                     TLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIVSKLDFAY
                     QFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESRRPELVRR
                     VEWAQEVMLADAELPFEPEFIDDLATKGLHTLFDVVTRRHEQLVVELGLEETAARRLL
                     DVCYARSDAWEKENGGTIYVR"
BASE COUNT          505 a          464 c          408 g          537 t
ORIGIN      
        1 tatattcgca cgtctttaaa acgcgaatac gccaccgaaa atggcacaga attaaacgaa
       61 acattaccaa aacttagtcc gttgaatccg caatataaaa cgaaaaaaca ggcagttttc
      121 cagaaaatcg tctcgtttat tgagaagttt aaaggcgtag gcggaaaaat atagcccaat
      181 tcgtgttttt cttgcgggtt cttaattaaa cccgcaagag ctcgtggggt tccaaatggc
      241 taatatactc cccttaccca tgcgcgacga tgccgccaaa agtgatagag aacagccaga
      301 aatagatcgc ggtcataatg attttgaatg ccgtgttcat atcttcagct cctgtgattg
      361 attggatata tgccgcgccc cttgcggcat gtttttattt tcactctctc tgttttaaaa
      421 atcaagattt attagagcaa ttattgttga tgaagaagcg cattttcata ctccctgacc
      481 attaacgtaa gtacgccgtg actcctgaaa acacgcgcca cttcaatctt atcttccagc
      541 gcgaacgcaa ttttacttag accaattttc ttcaggagat caatctttgc tggaccgtca
      601 tttctgtcat cggtggcagg acgcatagat agcaaaggct cagccccatt tgttacgtgc
      661 ttacgcaacc aggctcgtgt tttatccctg gctatctcac agcgcccggt tacaaaccag
      721 agggtgtaaa tgccggacaa ctggcgcacc atatcaataa ctggagtgat gggagcatca
      781 gtgtcacagg caaggttaaa ctcgttccag tgctctgtta atgcaccttt acctggtggc
      841 ggaagcaaat gcagtctgtc ttcagttgcc tctgatattg ttccatcaat atcgactatg
      901 acgatgtacg gacgttcctg gtgtgcgtgt ttattgtaaa tactcaaatg ccctcctcat
      961 tggacgaaaa aaaatgctgg tgggcgcact ccaccagcat taaaagtgac actgtaacca
     1021 tcagcgaacg taaatagtgc cgccgttctc tttttcccat gcatcgctac gtgcatagca
     1081 aacatcgaga agtcttcttg ccgcagtttc ctctaaaccc aattcgacaa ccaactgctc
     1141 atgacggcgg gtaaccacat caaacagggt atgcagccct ttagttgcca gatcatcaat
     1201 gaattccggt tcgaacggca gctctgcatc tgccaacata acctcttgcg cccactcaac
     1261 tcgacggacc aattccgggc ggcggctttc catctcttta cagatcaatt catggaagaa
     1321 ctctacccaa ccttccggct ggaactcgcg gaaaattgcc aacggctgga agtttggcat
     1381 caaccattcg ttgattcgga tatcaatggc atagcccatg tcgcagcaga actgataagc
     1441 aaagtccagc ttagaaacga tataaggacg ctcgttattg aactctttag gcgatgagat
     1501 cccataagcc aggaggcgcg ggaagaagga gatttgccct aacgtcggat gaagtttgct
     1561 tgcagggaaa cggcgctcag taatgccata catttccttc ttgagcgtcg caaatttggc
     1621 attctcatta accagcgcgg taacctctgc ttttttatta gcaaatgcca cgcgcgcttc
     1681 gcttgcatct ttaatagttt ttttgagctg ttggttaagg tcagcgacct gcttacgcag
     1741 ttcctgtcgc tcgcttttag ctttgttata gcgtttctca aggttaaaag gatcaagttt
     1801 catgatctct ttatattgag attttagcgt tgaaatctgt gagttccgca gttcaaccat
     1861 cgcggtcatt tcattgagtt ttgtttccag ctcaatgctt atacgttcgg catt
//
LOCUS       DAOFQD010000288         1909 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.294, whole genome shotgun sequence.
ACCESSION   DAOFQD010000288 DAOFQD010000000
VERSION     DAOFQD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.294"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            1307..>1909
                     /locus_tag="QBF46_004851"
     CDS             1307..>1909
                     /locus_tag="QBF46_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HDQ6676923.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVP
                     LRCPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRI
                     WRKLHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRK"
BASE COUNT          538 a          454 c          367 g          550 t
ORIGIN      
        1 acttagacaa catataacac cacttttccc tgcacctgtc ggcaagctta taagacatga
       61 tccagatgag cgcgatctaa aatattttgc agctacacca atactttcca cttgacatga
      121 tctgagtttt tcttctactt cagtaggtgt accattgtac atttaaattc ctcttaaaat
      181 gaaaaactgc tggattagaa acttatataa ttgtgtttat ataagcacgg gcaaggggcc
      241 gacaaattaa tttgtcagtt agctggctac tacggactga gcttatctcc cttctggcaa
      301 aaccagtcag tcttgattag tacgatgaaa acagccatac gactctttgc tctcttcctc
      361 cacatgaatt ccggcttctg ttaaattaaa agcaaaaatt tatagtgtcg ttaggcatga
      421 gatctaaggc tgcatacgat ttaaattagg ttttaaaatt taccgcgtta gtgcaatatg
      481 tactttattc atgcgcccta gaatctacca gcacctcact cacaaacttt ctgttttaaa
      541 tctccatgag tgcatcacag cagtcatcgg tacgaaccgt tgattcgctg acatctgtaa
      601 tgacgttctg acagtaaggt atcatcagcg ttatacgcat gaatctctac aaaattccta
      661 gctgatttgc acatccttgt caataattta attaagaacg ctaccgccct cagagacata
      721 agccttgata aaatctatgc tatcgcaacc aattttttat tcaatagatc aatcagataa
      781 ccatctaatg ctatcatgtg ttcgttgtga aaatgtgatg taagatggtt ttatgaaccg
      841 gaactaactg tcagctaatg ctagatacat agttttagtt catgaagtgt attactcatg
      901 gaaacttaga atctttgaaa aaacaataca gtagccacaa aattatccat gaacctagtt
      961 tttactcaac ataagttaaa tttagtccgt cggtcattta gttcataacc ggccacagga
     1021 tacgattaat catacactta cctacccctc tatcttcttc ttgcaggacg ttatgtttgt
     1081 cggttacttt gttcatatcc acaccattga taaggttaag tgccgcgtgt tcatacttac
     1141 acaactcaat atcaaccaac ggcagcgttt atgggcactg atggatactc acacgcgcca
     1201 gccgctttta tatccgttaa tatatcttat cgaccatctt gctttgcgtt catcagccac
     1261 gcaatccgct tcacggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat
     1321 cacgcatcct cccgacaaca cagaccattc cgtggcaaag caaaagttca gaatcaccaa
     1381 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga
     1441 tgaagcgatt caggcctggt atgagtcggc aacgccttca tcacgaggaa ggccccagcg
     1501 ctattctgat ctcgccatca ccaccgttct ggtgattaaa cgcgtattcc ggctgaccct
     1561 gcgggctgcg cagggtttta ttgattccat ttttgccctg atgaacgttc cgttgcgctg
     1621 cccggattac accagtgtca gtaagcgggc aaagtcggtt aatgtcagtt tcaaaacgtc
     1681 cacccggggt gaaatcgcac acctggtgat tgattccacc gggctgaagg tctttggtga
     1741 aggcgaatgg aaagtcagaa agcacggcaa agagcgccgt cgtatctggc gaaagttgca
     1801 tcttgctgtt gacagcaaca cacatgaagt tgtctgtgca gacctgtcgc tgaataacgt
     1861 cacggactca gaagccttcc cgggccttat ccggcagact cacagaaaa
//
LOCUS       DAOFQD010000289         1892 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.296, whole genome shotgun sequence.
ACCESSION   DAOFQD010000289 DAOFQD010000000
VERSION     DAOFQD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.296"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1863
                     /locus_tag="QBF46_004852"
     CDS             <1..1863
                     /locus_tag="QBF46_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDQ6676924.1"
                     /translation="DNITKLKSLFPEAFNEGSVDFEVLKQLLGENVDDKEERYGLNWH
                     GKRQARQLALTPSRGTLRPCKDESVDWDNTKNLMIEGDNLEVLKLLQKSYVNRVKLIF
                     IDPPYNTGKDFVYPDNFQDNIKNYLEITGQTEEGIYVGSNVETSGRYHTDWLNMMYPR
                     LILAKNLLTQDGVIFLSIDGTEVSNLEHLLNNVFGEENKITSICWQKKVSPANDAKWF
                     SSDHDFILVYAKNKDIWRPKKLKRTGEQLSYYKNPDHDPKGEWNSATYTCNKSMHERP
                     NLYYPIVNPNTMEEVWPKETAVWKYSKDITEKYINEKRLYWGIDGLAKFPRVKLYLSE
                     MDDVVPRSIWNYGDVGHTQQATNEIKELFSGEQVFDTPKPLKLLRRIVQLSSNKNDDI
                     ILDFFAGSGTTAHAIFEQNKEDGISRKSIIVQLPEVLLERNREQKAAYSFCMKNNLQP
                     TISELTKERLRRAGKKVREDNPDWNGDIGFRVFKLDTSNIRPWEATAESLSLQLDAYI
                     SPIIEGRSEEDLLAELILKRGIDLSVNVETRQFDGLTVSCVDGGKLFTCFTRQIPASS
                     VEALTKGIIDWHKSLKAGKDTVCYFLDDAFENNVAKTNLCAILEQHGLTNLHCL"
BASE COUNT          652 a          326 c          379 g          535 t
ORIGIN      
        1 gataacatca ccaagcttaa atcattgttt cctgaagctt ttaatgaagg cagtgttgat
       61 tttgaggttt taaagcagct tttgggtgag aatgtagacg acaaagaaga acgctatggc
      121 ctgaactggc atggtaagcg tcaggctcgt cagcttgcgc ttaccccttc tcgtggcacc
      181 ttacgcccat gtaaagatga aagtgttgat tgggataaca ccaaaaatct catgatcgaa
      241 ggtgataacc ttgaagtgtt aaaacttctt caaaaaagtt atgtaaatag agtaaagcta
      301 atatttattg accctccgta taatacagga aaagattttg tttatcctga taactttcaa
      361 gataatataa aaaattatct tgaaataaca ggccaaacag aagaaggaat atacgtaggt
      421 tcgaatgtag aaactagtgg acgttatcat actgattggt tgaacatgat gtatccacgc
      481 cttattttag caaaaaactt attgactcaa gatggagtta tttttctatc gatagatgga
      541 acagaggttt cgaatctaga acacttgtta aataatgtat tcggagaaga aaacaaaatc
      601 acttctatct gttggcaaaa aaaagtgtct cctgcaaatg atgctaagtg gttcagttcg
      661 gatcatgatt tcatcttggt ttatgcgaag aataaagata tttggagacc caaaaagctt
      721 aaacgtactg gcgaacagct atcttattat aagaaccctg atcatgatcc taaaggagaa
      781 tggaattctg caacatacac atgtaataaa agcatgcatg agaggccaaa cctttattac
      841 ccaatagtaa acccaaatac tatggaagag gtatggccta aagaaacagc agtatggaaa
      901 tactcaaaag acataactga aaaatatata aatgaaaaac gtttatattg gggaattgat
      961 gggcttgcaa aattcccgcg tgtaaaatta tatctttcag aaatggatga tgttgttcca
     1021 agatccatct ggaattatgg cgatgtaggt catactcaac aagcgacgaa tgaaataaag
     1081 gaactatttt ctggtgaaca agtatttgat acaccaaaac cactaaaatt gttaagaaga
     1141 atagttcaac tatcatcaaa taaaaacgat gatatcattt tggatttttt tgcgggttca
     1201 ggaacaacag ctcatgctat ttttgaacaa aacaaggaag atggcatttc tcgcaaaagt
     1261 attatcgtgc aacttcctga agttttatta gaaagaaatc gtgaacagaa agccgcgtac
     1321 tctttttgta tgaaaaacaa tctccagcca acaataagtg aacttactaa agagcgcctg
     1381 cgtcgtgcag gtaaaaaagt tcgtgaagac aatccagact ggaatggtga cattgggttc
     1441 cgtgtattta aacttgacac atccaatatt cggccttggg aagcaacagc ggaaagcctt
     1501 tcactacaac ttgatgccta tataagccca attattgaag gccgtagtga agaagaccta
     1561 cttgctgaat taatacttaa gcgtggcatt gatctgagtg taaacgttga aacccgtcag
     1621 tttgatggat taactgtttc ttgtgttgat ggtggaaaat tgtttacctg ctttacccgt
     1681 caaatccctg cctcttctgt tgaggcacta accaaaggga tcattgactg gcataagagt
     1741 ttaaaagctg gcaaagatac ggtctgctat ttccttgatg acgcatttga aaataacgtt
     1801 gctaaaacaa atctttgcgc cattctggaa caacatggcc tgactaacct acactgcctg
     1861 taagggaatg agatgaaact acattttgaa tc
//
LOCUS       DAOFQD010000290         1770 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.297, whole genome shotgun sequence.
ACCESSION   DAOFQD010000290 DAOFQD010000000
VERSION     DAOFQD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.297"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>1770
                     /locus_tag="QBF46_004853"
     CDS             <1..>1770
                     /locus_tag="QBF46_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDQ6676925.1"
                     /translation="SNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSGAYQKLTIGSD
                     WGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYGVP
                     PDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCNQLLKSLYY
                     DVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTDEIIRILEYIDNTLITFCNEEI
                     IKCQIAIQSIISKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRTFATALANIEK
                     YPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECDLNLVSGESLI
                     RQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQKLSQNKINKF
                     NNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICPYALMVYGVGD
                     GGAGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTHADAFRESLPIISGELYFEA
                     HQGCFTSESATKAHNRIMENKLHDAEFFITITNNMTSILRSEFDEIWKAMLTLQFHDI
                     LPGSCISRVYHETEKEYLKLEAK"
BASE COUNT          548 a          348 c          365 g          509 t
ORIGIN      
        1 tgtcgaataa aataggtcag cgtgtttata ctgttgtctc tgaaatgtcg attgaagcat
       61 ggataacaaa agaaccgcag ccttttgttt atcgccgtag cggcgcttac cagaaactca
      121 ccattggctc agactggggt caactgtttg actgtgcctg gatgcgagtt tatggtaaac
      181 gtccagcaga taaatttaga cacaaactcg tcgctctcgt tgatatcggt ggcgaaggtc
      241 ttattgtgga taagaatggt tcacccatct gcggcatcac gaataaagca tcatcttatg
      301 gcgtaccgcc agataaacca ggtaaatggg tggttgacct ttctctcgtt agtgaaaatg
      361 acgaggtcga gttttggatt gatgctgcgt gcaacgattt atttggctat gtcactaatg
      421 gagggattat tactgatgtt catatcgcga catgtaatca actactgaaa tccctttatt
      481 atgatgttga agtgcttttc gactggatta atgatggtca gaagtttgaa tccatacatc
      541 ctaaaggtat tatcccagaa aaaattatca ccaaaagaag cgagcgtact gacgaaatca
      601 taagaattct cgaatatatt gataatacgt taattacctt ttgcaatgaa gaaataatta
      661 aatgtcaaat tgcaatccaa tccataatta gtaaaaacaa taatccttca gagttcagga
      721 ttatggctac tggtcatgcc catttagata tagcgtggat gtggccttta agagaaggac
      781 gcagaaaagc aataagaaca tttgccaccg ccctggcaaa cattgaaaaa tatcctgatt
      841 acatttttgg cgctagtcag taccaacttt ttcactggat aaaaaaagat tatccctatt
      901 tcttcgaaaa gttaaaaaaa caaatcgccg caggccgctt tgagttacaa ggttgctttt
      961 gggtcgaatg cgatttaaat ctggtaagtg gtgaatcgct gataaggcaa atcatgcatg
     1021 ggacacgatt cacactgcag aatttttcaa agaagataaa ttatgtctgg caaccggatg
     1081 tctttggatt ccccgccacg cttccacaaa ttctcaaaaa atcaggcatt aactatattg
     1141 catcgcaaaa gctctcacaa aacaagatta ataaatttaa taattacctt ttccgctggc
     1201 aaggcctgga tggctcagaa attctgatgc ataactttcc tgaagatacg tatgatagcc
     1261 gagcccgcgc tcgctcttta gaatatattg aacaaaacta taatgaaaaa gaaatctgtc
     1321 cctatgcgct tatggtgtat ggtgtcggtg atggcggcgc tggcggcgct ggccccggtg
     1381 aggaacatat agaacgatta acgcgcatac gcaatattga tggcttacct catgttgatt
     1441 tcagccgtgt tgataagttc ttcactcacg ccgacgcctt tcgcgaatcc ttgcccatta
     1501 tttcgggtga actttacttc gaggctcatc aagggtgctt tacatcagag tcagcaacta
     1561 aagcccataa tcgaattatg gaaaataaac tccatgatgc tgaatttttt ataactatta
     1621 ctaataatat gacgtcaatt cttcgcagtg agtttgatga aatatggaaa gcgatgctga
     1681 ccttgcaatt ccatgacatc cttcccggct catgtatttc aagagtttat catgaaactg
     1741 aaaaggaata tcttaaacta gaagcaaaaa
//
LOCUS       DAOFQD010000291         1728 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.299, whole genome shotgun sequence.
ACCESSION   DAOFQD010000291 DAOFQD010000000
VERSION     DAOFQD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.299"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..344
                     /locus_tag="QBF46_004854"
     CDS             <1..344
                     /locus_tag="QBF46_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="catalyzes the reduction of trimethylamine-N-oxide
                     to form trimethylamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDQ6676926.1"
                     /translation="RNGDIVRVFNARGQVLAGAVVSDLYSPGVARIHEGAWYDPDNGG
                     ETGALCKYGNPNVLTIDIGTSQLAQATSAHTTLVEIEKYTGKVDNVTAFSGPIKMVAQ
                     CEYVPAEKVSS"
     gene            341..955
                     /gene="torD"
                     /locus_tag="QBF46_004855"
     CDS             341..955
                     /gene="torD"
                     /locus_tag="QBF46_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDQ6676927.1"
                     /translation="MIKHSSLTTEQMACVYAWLAQLFSRERDDDSLALLQSSEMAEWF
                     AVLKGEPSLEAEVLQLEEKIAALQVREDATLELAADFCSLFLMTEKHAALPYASAHLE
                     GGPNYGVIKRLLADAGMQVSETFSESADHLAIFIELLSHLHFSLGESGLSRQRIDALR
                     RETLAGLLRWLPEFTAKCRRYDDFGFYGALSQLLLALVRLDIQN"
     gene            complement(1196..1624)
                     /gene="uspF"
                     /locus_tag="QBF46_004856"
     CDS             complement(1196..1624)
                     /gene="uspF"
                     /locus_tag="QBF46_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDQ6676928.1"
                     /translation="MYRSILVPIDISETDLTRHVVPQVLAHAKTAKVHFLAVIPTVPF
                     YASLGLAYSTEFPDRSGLQAKASEKLEEIIKQFNIPAGRSQTHVVYGPPKDQILKLAE
                     AVDAELIVIASHKPGFSTYLLGSTAAAVVRHAKCPVLVVR"
BASE COUNT          431 a          411 c          476 g          410 t
ORIGIN      
        1 ttcgcaacgg tgatattgtc cgtgtcttta acgcacgtgg acaagtactg gccggggcgg
       61 ttgtctccga tctctactcg ccaggcgtgg cgcgtattca tgaaggtgcc tggtacgacc
      121 ctgataacgg cggagaaact ggggcgctgt gtaaatacgg caatccgaac gtattaacaa
      181 tcgatatcgg cacgtctcaa ctggcacagg cgaccagcgc tcataccacg ctggtggaaa
      241 ttgagaaata taccggcaag gtggataacg tgacggcgtt cagcggcccg ataaaaatgg
      301 tggcgcagtg cgaatatgtt ccggcggaga aggtatcatc atgataaaac actcttccct
      361 gacaacagaa cagatggcct gcgtttacgc ctggctggcg cagttgtttt cccgggaacg
      421 tgacgatgac agcctggcac ttttgcagtc cagcgaaatg gctgagtggt tcgcggtgct
      481 aaaaggtgaa ccgtcgcttg aagcggaagt ccttcagctg gaggagaaaa ttgctgcgct
      541 tcaggtgcgt gaggacgcaa ccctcgaact ggcggctgat ttttgtagcc tttttctgat
      601 gacggaaaaa cacgcggcgt taccctatgc gtcggcccat cttgaaggag ggccgaatta
      661 cggggtcatt aaacgattgt tagccgatgc tggcatgcag gtaagcgaaa cgtttagcga
      721 gtcagccgat catctggcaa tttttatcga actgctgagt catctgcatt tttctctggg
      781 agagtccggg cttagccgcc agcgtattga tgcgctgcgc agagaaacgc tggcggggct
      841 gttacgctgg ttaccggaat tcaccgccaa atgccgccgc tatgatgact ttggtttcta
      901 tggcgcgctg agtcagctgt tgctggcact ggtcaggctg gatattcaga actgagtgat
      961 ggcctaagcc acagaaaaac gggaatacat tcgctctaat gcttgccatt ttaacgaaaa
     1021 tgaaagggcg taatgagtaa ctgagagtgg gacatccgga ggcaatgaat ctgttgcctc
     1081 cggtatattg ctacgtttac cactgaggtg aacggaaccc tgagcaagaa tcacatgtgc
     1141 tcaagaatgc ttaacttata aacgttagtg caaattcgcc cccattttga ccctatcacc
     1201 gcacaaccag cacgggacat tttgcatggc gtactacggc agccgccgtt gaacccagca
     1261 gataagtgct aaaaccaggc ttatgtgagg caataacaat aagctcagcg tctaccgctt
     1321 ccgccagttt gaggatctga tccttcggcg ggccgtaaac gacgtgagtc tgactcctgc
     1381 ctgcaggaat attgaactgc ttaataatct cttcgagttt ttcgctggcc ttggcctgca
     1441 gaccgcttct gtccgggaat tctgttgagt aagccagccc cagcgaggca tagaacggaa
     1501 cggtaggaat aacggctaaa aagtggacct tagcggtttt ggcatgcgcc aggacctgtg
     1561 gaacaacatg gcgggttaaa tcggtttcag agatatcaat gggcaccaga attgagcgat
     1621 acatattcac tacctcctgg ttgtttacct ctacccagag tgtagcgaaa atcgaatctg
     1681 tataataagt gaccagatca aggttgcatg cataaatgcg cgtcaatg
//
LOCUS       DAOFQD010000292         1684 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.300, whole genome shotgun sequence.
ACCESSION   DAOFQD010000292 DAOFQD010000000
VERSION     DAOFQD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.300"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            37..777
                     /locus_tag="QBF46_004857"
     CDS             37..777
                     /locus_tag="QBF46_004857"
                     /inference="COORDINATES: protein motif:HMM:NF016370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDQ6676929.1"
                     /translation="MFNLTIDESASPVSRETVERTAYSLGVSSSTFTPLPYNELSKKL
                     GYKASLLMLCLSKNPNVSLPALKGTVELISTLTEYPAFKVNSLTTSNNAYPVFTLETL
                     AANGIFSNDLEFSTNISHCFRISPELKVPKISLGLNARGSYELDLSLELVIIKELIEY
                     SVAKVIVEFDGPMHLNSDNVRNDKLRDSYVQSQGATVFRIQSVNTHDLTKIQQTEKSH
                     EQLMAHIGNIKEHFRRRLFEFLKTFHNS"
     gene            complement(800..>1684)
                     /locus_tag="QBF46_004858"
     CDS             complement(800..>1684)
                     /locus_tag="QBF46_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ6676930.1"
                     /translation="KAVRLRYAFIRDNTCCWPVRLLCRVLDVHPSGFYAWLQQPHSQR
                     HQTDLRLTGQIKQFWLESGCVYGYRKIHLDLRDTGQQCGVNRVWRLMKRAGIKAQVGY
                     RSPRARKGEACIVSPNRLQRQFNPDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKI
                     IGWSMQSRMTKDIVLNALLMAVWRRNPQKQVLVHSDQGSQYTSHEWQSFLKSHGLEGS
                     MSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSSD
                     QMPPTEYENQYYQRLRSV"
BASE COUNT          467 a          396 c          358 g          463 t
ORIGIN      
        1 gtatcaaagg acagcattgc tactaggata acgagaatgt ttaatttaac catcgatgaa
       61 tcagcgtcgc cagtttcccg cgaaaccgtt gagcggacag cctattcttt gggagttagt
      121 agttctacgt tcactccatt accttataat gagctatcaa aaaaactagg atataaagca
      181 agtttgctaa tgctatgcct ttcaaaaaac cctaatgtca gccttcctgc attaaaaggg
      241 actgtagaat taatttctac actaactgaa tatccagcgt tcaaagttaa ctcgttaaca
      301 acatctaata atgcctatcc agttttcaca ttggagacat tggctgctaa cggtatattt
      361 tcaaacgatt tggaattttc tacaaatatc tctcattgct ttcgaattag tccagaatta
      421 aaagtaccaa agatatctct aggtttgaat gcaagaggat cgtatgaatt agacttatcc
      481 ttggaattgg tgattattaa agaattgatt gaatactctg tagcaaaagt gatcgttgaa
      541 tttgatggcc cgatgcatct taattcagat aatgttcgta atgataaact aagagattct
      601 tatgtccaaa gtcaaggggc tacggttttt cgtattcaga gcgtgaacac acatgatctg
      661 accaaaatcc agcaaaccga gaaatcacat gaacaattga tggcacacat tggaaatatt
      721 aaagaacact tccgcaggag actattcgaa tttttaaaaa cgtttcataa tagttgatta
      781 gtgaatcgcc acggataatc tagacacttc tgagccgttg ataatattga ttttcatatt
      841 ccgtcggtgg catctgatca ctcgaaccat gccggcgctt actgttataa aacatttcga
      901 tgtaatcaaa aatatcgctg cgggcttctt cccgcgttcc atagatcttt ttctttatcc
      961 gttcacgctt cagcaactgg aaaaagcttt ctgcaaccgc attatcgtgg cagttaccgc
     1021 gacggctcat gctgccctcc agaccatgtg atttcaggaa cgactgccac tcatggcttg
     1081 tgtactgact gccctgatca gagtgaacca gcacctgttt ttggggatta cgccgccata
     1141 cagccatcag caatgcgttc aggacaatgt cctttgtcat ccgggattgc attgaccagc
     1201 cgataatttt gcgtgagaac agatcaacaa ccacggcaag atacagccac ccttcgtggg
     1261 tcctgatgta ggtgatgtcc gttacccaac gctcgtccgg agcatccgga ttgaactgtc
     1321 gctggagcct gttgggcgac acgatacagg cctcgccttt acgtgcccgc gggctccggt
     1381 atccgacctg agcctttatc ccggcacgtt tcatcagtcg ccagactctg ttgactccgc
     1441 actgttgccc ggtatcccgt agatccagat ggatcttgcg ataaccatag acgcatcccg
     1501 actccagcca aaactgttta atctgtcctg tcagtctcag gtctgtctga tggcgttgtg
     1561 aatgcggctg ctgaagccag gcgtaaaagc cactgggatg aacatccagc acccgacaga
     1621 gcaggcgaac aggccagcaa caggtgttgt cacggataaa ggcgtacctc agtcggacag
     1681 cttt
//
LOCUS       DAOFQD010000293         1683 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.301, whole genome shotgun sequence.
ACCESSION   DAOFQD010000293 DAOFQD010000000
VERSION     DAOFQD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.301"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(168..761)
                     /gene="tehB"
                     /locus_tag="QBF46_004859"
     CDS             complement(168..761)
                     /gene="tehB"
                     /locus_tag="QBF46_004859"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDQ6676931.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTWVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEKVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(758..>1683)
                     /gene="tehA"
                     /locus_tag="QBF46_004860"
     CDS             complement(758..>1683)
                     /gene="tehA"
                     /locus_tag="QBF46_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDQ6676932.1"
                     /translation="GFAWRYASQVWQVSHWLGDGLVILAMIIWGLLTSAFITRLIRFP
                     HSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFRPLAVCLFSFGVVVQLAYAAWQTA
                     GLWRGSHPEEATTPGLYLPTVANNFISAMACGALGYTDAGLVFLGAGVFSWLSLEPVI
                     LQRLRSSGELPTALRTSLGIQLAPALVACSAWLSVNGGEGDTLAKMLFGYGLLQLLFM
                     LRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGSGSDNGFFHTLAVPLFIFTNFIIA
                     ILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
BASE COUNT          470 a          448 c          383 g          382 t
ORIGIN      
        1 gggctaagtc aatcatcagt agttaaattt cagttagtta agctgctaaa aaacttcgac
       61 tactgtgatt aatttcacat cccttcgata aaaaatatgc ttaccgaaaa gaagaacagt
      121 aaaccatctc agcgtgggga attttcacgc gctttgttct taccgggtca ttttttacgt
      181 gccagcatcg tggcgaaacg tagtttaata cgattaccgt tggcgtcggt gcggtgcagc
      241 tcgccgacgt cttcattgta tttcaccttc tcccagcctt cgtaatatcg acgtaattct
      301 ccctctttga aggcaaacgg gaagccgacg gtacatggat aatcagcggt atccatcgcc
      361 gccacaatca ggttgtaacc gccaggtttc gtgcaacgtt gcatattggc aatcagccct
      421 gggatggttt tagcctcaag gaacatcagc accacagtcg aaagaataaa atcgtactgt
      481 ccatcaaatg tgaggttatt cagatcaacg acccaggtgt gtaaattatc cagattttca
      541 atggatttaa tgcgctcgac gttggcgata ctcatggcat ttttatccca tgcgtcaaca
      601 tcataaccat tggctgccag gtaaagactg ttacgaccat tgccacagcc cagatccagc
      661 gttttacccg gtttaaccac tttcaccgct tccagtactt cagagtgtgt gcgggttaat
      721 tcatatttat cagtaaaata gttttcgtca cgaatgatca ttctttgtcc tctgctttca
      781 ttaaaacggc gcgctcggtt ctgactaaca attttccctg catcagaagc gcaaaagtac
      841 ggatgagcag tattgcaata ataaaattgg taaagataaa cagcggcacc gccagcgtat
      901 ggaaaaatcc attatcgctg ccactcccca gatgcaaacc ggtggttgcc agtgcagata
      961 cgccgaacga gaaactccag aatgaagcat taaatggctg ggagagatac catggcatca
     1021 gacgtagcat aaacagcagt tgcagcagcc cataaccgaa aagcattttc gccagcgtgt
     1081 caccctcgcc gccgttgacg ctcagccagg cactacaggc caccagcgca ggagcgagct
     1141 gaatgccgag tgatgtccgc agtgccgtgg gtaattctcc cgaactgcgc agacgctgca
     1201 agatcacagg ttcaaggctt agccatgaga aaacgcctgc gcctaaaaac accagaccgg
     1261 cgtcggtgta gcccaacgca ccacaggcca ttgcgctgat aaagttgttg gcaactgtcg
     1321 gcagatacag tccaggcgtg gtagcttctt cagggtgaga tccgcgccat aatcccgcag
     1381 tttgccaggc ggcataagcc aactgaacca cgacaccaaa actgaacagg cacaccgcca
     1441 gtgggcgaaa ccacggaaca aaaccaatcg ccaccagcat cgtcgttgcc ggaaacaaac
     1501 tcacaaaact gctcagcact ggatggcgaa cttccgccag cacgctatgc ggaaagcgta
     1561 tgagtcgggt aataaatgcg ctagttaata atccccagat gatcatcgcc agaatcacca
     1621 gcccatcccc taaccagtgg ctgacctgcc aaacctggct ggcatagcgc caggcaaatc
     1681 cca
//
LOCUS       DAOFQD010000294         1672 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.302, whole genome shotgun sequence.
ACCESSION   DAOFQD010000294 DAOFQD010000000
VERSION     DAOFQD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.302"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            33..521
                     /locus_tag="QBF46_004861"
     CDS             33..521
                     /locus_tag="QBF46_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HDQ6676933.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            604..>1672
                     /gene="ugpC"
                     /locus_tag="QBF46_004862"
     CDS             604..>1672
                     /gene="ugpC"
                     /locus_tag="QBF46_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDQ6676934.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLCRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIHIAQNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSTNRKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLYFNLD"
BASE COUNT          521 a          309 c          359 g          483 t
ORIGIN      
        1 actaagctat actactttta ttgagagttt aaatgaaaaa taaattgctt attgcggctg
       61 tgttgactgg tcttctttca ggatgcgttg atggcaattc accctctggc agtcaggaag
      121 gtgtcgtgaa agcggatccc cgctattcag aatgtaacct cccagatgta aatgaaagag
      181 ggccaattgc tgcgggtctt tatatctctg gaactctccc ggaaggctat tggttaatga
      241 agccagagag aaaaatgcat tataagggag atggtattta tcagcttatt tattcagaaa
      301 aagccggtgc ggtaacaatg caatttgcga ctatgaactg gaacccgcag ttcactgtaa
      361 caggtcttga attgccatta aatcaggcta aaacacttaa aaaaggcggc atggataaaa
      421 atacaaaagt cgtcattcct gccgatggaa agtatgtttg gacgattcaa attggtcctg
      481 ataaaaaggc aatagccgcc atggtagcac catgtaaata gttattttta gcactccacc
      541 attcaacatt cgttgaatgg tggaggatca gtattccaaa attcttgtta atgagagcag
      601 attatggccg cggtaattct acgcaaagtt gaaaaaattt atgacaatgg attcaaagct
      661 gttcacggca ttgatctcga tataaacgat ggtgaattca tggtttttgt tggaccatct
      721 gggtgtgcca aatcaacgac attacgcatg attgcaggtc ttgaagacgt atcaggtggt
      781 gaaatttaca ttagcgaccg ccttgttaat acccttccgc ctaaagatcg cagcattgca
      841 atggtgttcc agaactatgc gctgtatccc cataagactg tatttgacaa tatggctttt
      901 ggtttaaaaa tgcaaaaaag gccaaaagaa gagattaaga aaattgtcga agaagttgca
      961 gaaaaacttg aaattagcga tttactttgc cgaaaaccga aagaaatgtc gggtggtcag
     1021 cgtcagcgtg tcgcggtagg ccgtgcaatt gttcgtaaac cagatgtttt cttatttgat
     1081 gagccactat ctaaccttga tgctaaactt cgtgtttcaa tgcgagtcaa aattgctcag
     1141 ttgcatcagt ctctcaagga tgaaggtcat cctgcaacca tgatttatgt tacacacgat
     1201 caaattgaag cattgaccct cggtgatcgt atttgtgtgc ttaatcaggg gcgaataatg
     1261 caggtcgaca caccagttaa tctgtataac caccctgcta acagattcgt tgctggcttt
     1321 attggttccc cgtcaatgaa tttgatagat gcggttgtac gcgaggaaga cggtaaactc
     1381 tatattcaca ttgcccaaaa tattcggttg tacatacctg agaataaaca atcagtactc
     1441 aaagcgtata tagataaacc cgtttgtttt ggtattcgtc cagaacatat ctctttgagc
     1501 acgaatcgca aagaaatgaa cactgttact ggtgcattaa gtattattga aaatatgggg
     1561 agtgaaaaat atctttactt taccgttaac aacaaagagt ttattgctaa agtcaacgat
     1621 caatcaatta ctacagccga tattggaaaa aatttgtatt tcaaccttga ta
//
LOCUS       DAOFQD010000295         1654 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.303, whole genome shotgun sequence.
ACCESSION   DAOFQD010000295 DAOFQD010000000
VERSION     DAOFQD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.303"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(92..907)
                     /locus_tag="QBF46_004863"
     CDS             complement(92..907)
                     /locus_tag="QBF46_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDQ6676935.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1044..>1654
                     /locus_tag="QBF46_004864"
     CDS             1044..>1654
                     /locus_tag="QBF46_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HDQ6676936.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWSTYNKALINRGSLTFWLD
                     DGAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVP
                     LRCPDYSCVSRRAKSVNISFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRA"
BASE COUNT          460 a          403 c          366 g          425 t
ORIGIN      
        1 gtccggatac tgccagatat cggcgtccag ggagatgtcc tgaagcgcgg tgtccggaat
       61 ttcaggtttg tgtctctaca aagactaact atcagaaaaa ctcatcgagc atcaaatgaa
      121 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
      181 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
      241 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
      301 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
      361 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactag
      421 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
      481 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
      541 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
      601 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
      661 tcggaagagg cataaatgcc gtcagccagt ttagtctgac catctcatct gtaacatcat
      721 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
      781 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
      841 aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat
      901 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
      961 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt
     1021 gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag
     1081 accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc
     1141 tcatcaaccg tggctccctc actttctggc tggatgatgg ggcgattcag gcctggtatg
     1201 agtcagcaac accttcttca cgaggcagac ctcagcgcta ttctgacctt gccatcacga
     1261 ctgtgctggt cattaaacgc gtattcaggc tgaccctgcg cgctgcgcag ggctttattg
     1321 attccatttt tacactgatg aatgttccgt tgcgctgccc ggattacagc tgtgtcagca
     1381 ggcgggcaaa gtcggttaat atcagtttca aaacgttcac ccggggtgaa atcgcgcatc
     1441 tggtgattga ttccaccggg ctgaaggtct ttggtgaagg cgaatggaaa gtcaaaaagc
     1501 atggccagga acgccgccgt atctggcgta agctgcatct cgccgttgac agtaaaacac
     1561 atgaaatcat ctgcgctgac ctgtcgctga acaatgtgac cgactcagaa gccttcccgg
     1621 gtcttatccg gcagactcac agaaaaatca gggc
//
LOCUS       DAOFQD010000296         1634 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.304, whole genome shotgun sequence.
ACCESSION   DAOFQD010000296 DAOFQD010000000
VERSION     DAOFQD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.304"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..472
                     /gene="cas6e"
                     /locus_tag="QBF46_004865"
     CDS             <1..472
                     /gene="cas6e"
                     /locus_tag="QBF46_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDQ6676937.1"
                     /translation="LSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICKAGK
                     RHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQLR
                     RENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            469..1392
                     /gene="cas1e"
                     /locus_tag="QBF46_004866"
     CDS             469..1392
                     /gene="cas1e"
                     /locus_tag="QBF46_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDQ6676938.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            1389..>1634
                     /gene="cas2"
                     /locus_tag="QBF46_004867"
     CDS             1389..>1634
                     /gene="cas2"
                     /locus_tag="QBF46_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDQ6676939.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVD"
BASE COUNT          392 a          386 c          480 g          376 t
ORIGIN      
        1 cctgtcgcag gaacgcccgg cggaaagtga tattttcacc atcgaatgcc gaccatttgc
       61 gccagagttg cgtaccggac aatccctttg tttcaacctg cgggcgaatc caacaatttg
      121 taaagcgggc aaacgccacg acttgctgat ggaggcgaaa cggcaggtga gggggcaggt
      181 ggaaggaagt gatgtctggt tgcatcaaca acaggctgcg ctggactggc tggcagcgca
      241 gggggagaga agcggtttta cactgctgga tacttcggtt gatgcctacc gacaacagca
      301 actgcggcgg gaaaactccc ggcaactgat ccagttcagt agcgtggatt atacggggat
      361 gcttacggtc accgctcctg ggttatttct gcaacgtctc agtcaggggt atggcaaaag
      421 ccgggcgttc ggttgtggtc tgatgctgat caaacccgga gcagaggcgt gacgtttgta
      481 ccgctgagcc cgatcccatt aaaagatcgc acttccatga tcttcctcca gtacggtcaa
      541 atcgacgtac tggacggcgc tttcgtgctg atcgacaaaa ccgggatccg cacgcatatt
      601 ccagtgggat cggtcgcctg cattatgctc gaaccgggaa caagagtttc ccacgcggca
      661 gtccatctgg ccgccacggt gggaacactg ctggtctggg tcggtgaagc gggcgttcgc
      721 gtttactcct ccggacaacc cggaggggcg cgggcagata aattactcta ccaggcaaag
      781 ctggctttaa cggaagatct acggttgaag gtggtgcgca aaatgtatga attacgtttt
      841 cgtgaaccac cgccagctcg ccgttcagtg gagcagttac gtggaattga gggatctcgc
      901 gttcgccaga cgtatgcatt actggcgaaa caatatggtg tgaaatggaa tggtcgcaaa
      961 tacgatccta aagactggga aaaaggcgat gttgtgaatc gctgcatcag tgctgctaca
     1021 tcatgtctgt acggtatatc tgaagcggca gtattagccg cgggatatgc gcccgctatt
     1081 gggtttatcc atagtggcaa accgctttca tttgtttatg acatagccga tatcattaaa
     1141 tttgattcgg ttgtgccaaa ggcatttgaa atagcagcga ggcaacccgc agaacctgat
     1201 aaagaagtca gattagcctg tcgcgatatt ttccgtagca ctaagttaac gggcaaatta
     1261 ataccgttaa ttgaggaagt ccttgctgca ggtgaaattg aaccaccaca acccgcgccg
     1321 gatatgttac cgcctgccat ccctgaacct gaaacgctgg gtgatagtgg tcaccggggg
     1381 cgcggcggat gagcatggtc gtggttgtta cagaaaatgt ccctccgcgc ttacgtggac
     1441 ggctcgcaat ctggctactg gaagtgcgtg ccggtgtgta tgttggtgat acatcaaaac
     1501 gtattcggga gatgatctgg cagcaaatta cccaactggc tggttgcgga aatgtggtga
     1561 tggcctgggc gaccaatacc gagtcgggtt ttgaatttca gacctgggga gaaaacagac
     1621 gtattccggt ggat
//
LOCUS       DAOFQD010000297         1608 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.305, whole genome shotgun sequence.
ACCESSION   DAOFQD010000297 DAOFQD010000000
VERSION     DAOFQD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.305"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>1608
                     /locus_tag="QBF46_004868"
     rRNA            <1..>1608
                     /locus_tag="QBF46_004868"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          433 a          353 c          499 g          323 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacgct
      601 tatgcgttgt tgggtagggg agcgttctgt aagcctgtga aggtgtgctg tgaggcatgc
      661 tggaggtatc agaagtgcga atgctgacat aagtaacgat aaagcgggtg aaaagcccgc
      721 tcgccggaag accaagggtt cctgtccaac gttaatcggg gcagggtgag tcgaccccta
      781 aggcgaggcc gaaaggcgta gtcgatggga aacaggttaa tattcctgta cttggtgtta
      841 ctgcgaaggg gggacggaga aggctatgtt ggccgggcga cggttgtccc ggtttaagcg
      901 tgtaggctgg ttttccaggc aaatccggaa aatcaaggct gaggcgtgat gacgaggcac
      961 tacggtgctg aagcaacaaa tgccctgctt ccaggaaaag cctctaagca tcaggtaaca
     1021 tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag aataccaagg cgcttgagag
     1081 aactcgggtg aaggaactag gcaaaatggt gccgtaactt cgggagaagg cacgctgata
     1141 tgtaggtgaa gtcccttgcg gatggagctg aaatcagtcg aagataccag ctggctgcaa
     1201 ctgtttatta aaaacacagc actgtgcaaa cacgaaagtg gacgtatacg gtgtgacgcc
     1261 tgcccggtgc cggaaggtta attgatgggg tcagcgcaag cgaagctctt gatcgaagcc
     1321 ccggtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
     1381 gttccgacct gcacgaatgg cgtaatgatg gccaggctgt ctccacccga gactcagtga
     1441 aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag acggaaagac cccgtgaacc
     1501 tttactatag cttgacactg aacattgagc cttgatgtgt aggataggtg ggaggctttg
     1561 aagtgtggac gccagtctgc atggagccga ccttgaaata ccaccctt
//
LOCUS       DAOFQD010000298         1587 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.306, whole genome shotgun sequence.
ACCESSION   DAOFQD010000298 DAOFQD010000000
VERSION     DAOFQD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.306"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(267..1022)
                     /gene="repE"
                     /locus_tag="QBF46_004869"
     CDS             complement(267..1022)
                     /gene="repE"
                     /locus_tag="QBF46_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HDQ6676940.1"
                     /translation="MAEIAVINHKKRKNSPRIVQSNELTEAAYSLSRDQKRLLYLFVH
                     QIRKSDGSLQEHDGICEIHVAKYAETFGLTSAEASKDIRQALKGFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGVVSLKIDWIMERYQLPQSYQRMPDFRRRFLKASVDEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTIE"
BASE COUNT          439 a          370 c          378 g          400 t
ORIGIN      
        1 gggaagaggg ggaaaatcta gtgtgacaaa ctaccctcag gataggatga caaattgccc
       61 tcaattctgg ctccactacc ggttacagcc aaaaaaccat gagtaagcgc aggatgccag
      121 ggagggaaca gcaaaactgt gacaaatcac cctcaacctt ccagatcaaa tgtgacgaac
      181 cacccttaaa tctgtgacaa atcaccctca accttccaga tcaaatgtga cgaaccaccc
      241 ttaaatctgt gacagataac cctcaactat tcaatcgtca tggaggttat atcacggaag
      301 gaaaatacga tatgcgtcgt ctggcggcct ttctttttct cgatgtaaga aaggcgcatt
      361 ggtgtccggc tgttgatctc gtcaacactt gccttcagga aacggcggcg aaagtccggc
      421 atacgctggt aactttgagg tagctggtag cgttccatga tccagtcgat tttcagggac
      481 acgacacctg agccatcagg tttacggtac tggcacagag attcgtataa acgcatggcg
      541 tacggattgg taatctcttt tgtttcactg agccggaact gcgtaaaccg gttctgtaac
      601 ccgatgaaga agggaatcag atatgggttg atatgtacgc tgtaaagccc tcttgatggg
      661 ctgtgcgcac gtttaataaa ccagggaaag gattcatacc ctttttcatc gccggcatcc
      721 tcttcagggc gatagaaaac cacttcctta cccgcaaaac cttttaaagc ctgtcgtata
      781 tccttactgg cttcagcgga ggtcaacccg aatgtttcag cgtatttagc aacgtgaatt
      841 tcgcagatgc cgtcatgttc ctgcagggag ccgtcggatt ttctgatctg gtgaacgaac
      901 agatacagca gacgcttttg atccctggag agactatatg ccgcctcagt cagctcattt
      961 gactggacaa tccgcgggct atttttacgt tttttatggt ttataaccgc tatttccgcc
     1021 atgacaggtc catgtgaagt gtgacagact ttaagattgt cacacttcat aaaacagagt
     1081 caacagggat ggataacttt gtgaaaagca acgcttcctg agggcaattt gtcacagggt
     1141 taagggcaac ttgtcacaga aagggcggct atttgagggt gatttgtcac actgaaaggg
     1201 cgttttgtca cagatccctc tctggaaccc gcatggataa aggcctgtgg gcattctaaa
     1261 aataaagatc taacaactat ttaaaaagat cttataaaaa tatccccgtg gataagtgga
     1321 taacccctag gggaggtgat tttgcaggca ttgagcgtat gcagaattta tgagtactgt
     1381 tccatggtgc ttcctcgctc actcggggct tcgccctgtc gctcggctgc ggcgagcact
     1441 gctggctcga ttatatctgt ggccagaagt tagacataca gcctattaat tacgtgatga
     1501 gcggcaggga aaaacaccaa aagcctcccc cattaccccg gaacaaattg aaatccgtga
     1561 gctcaggaaa aagctacaac gtattga
//
LOCUS       DAOFQD010000299         1569 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.307, whole genome shotgun sequence.
ACCESSION   DAOFQD010000299 DAOFQD010000000
VERSION     DAOFQD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.307"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(190..1050)
                     /locus_tag="QBF46_004870"
     CDS             complement(190..1050)
                     /locus_tag="QBF46_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HDQ6676941.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1233..>1569)
                     /locus_tag="QBF46_004871"
     CDS             complement(1233..>1569)
                     /locus_tag="QBF46_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6676942.1"
                     /translation="DMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAE
                     RRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVY
                     KILEDERAS"
BASE COUNT          406 a          395 c          344 g          424 t
ORIGIN      
        1 gttgcacata gcccggcaat tcctttccct gctctgccat aaggggtctg acgcacagtg
       61 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
      181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
      241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
      301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
      361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
      421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
      481 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
      541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
      601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggcagcagt
      661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
      721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
      781 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcaccacata gcagaacttt
      841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
      901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
      961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1021 aagggcgaca cgaaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1081 ttaccagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     1201 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
     1261 ttataaaccg tggagcgggc aatactgagc tgatgagcaa tttccgttgc accagtgccc
     1321 ttctgatgaa gcgtcagcac gacgttcctg tccacggtac gcctgcggcc aaatttgatt
     1381 cctttcagct ttgcttcctg tcggccctca ttcgtgcgtt ctaggatcct ccggcgttca
     1441 gcctgtgcca cagccgacag gatggtgacc accatttgcc ccatatcacc gtcggtactg
     1501 atcccgtcat caatgaaccg gactgccacg ccctgagcgt caaattcctt tatcagttgg
     1561 atcatatcg
//
LOCUS       DAOFQD010000300         1545 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.308, whole genome shotgun sequence.
ACCESSION   DAOFQD010000300 DAOFQD010000000
VERSION     DAOFQD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.308"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            39..176
                     /locus_tag="QBF46_004872"
     CDS             39..176
                     /locus_tag="QBF46_004872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676943.1"
                     /translation="MSYTWKLADVPAWVDKKAFSSVKGMNEPEDGMINLVKTSNGWKA
                     I"
     gene            186..461
                     /locus_tag="QBF46_004873"
     CDS             186..461
                     /locus_tag="QBF46_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676944.1"
                     /translation="MENNTIKCPFCLKESQRGVHVCTGCMATVLYGTYPGWYAFVVIL
                     LTFGLSILIGMSTGMAGATISLPIIAAVGFVAGKAIFSDNVVFRRKM"
     gene            916..1131
                     /locus_tag="QBF46_004874"
     CDS             916..1131
                     /locus_tag="QBF46_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676945.1"
                     /translation="MKDVLDTLRQVREKSKHTVDNSSLKKLDDAILTLEKRIEENSKG
                     HTALDYLSYIAKIFALIKLLSGDSDFH"
     gene            1222..1503
                     /locus_tag="QBF46_004875"
     CDS             1222..1503
                     /locus_tag="QBF46_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005969687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6676946.1"
                     /translation="MLDIGSTIKLCREARKLTLQELSDRTDLTKSYLSRIENNQRDPT
                     ITALERISLALHIPLNIIILLSESEETNDEFSDINNMLKKTIMDTLVNA"
BASE COUNT          470 a          289 c          327 g          459 t
ORIGIN      
        1 ggactgaacc gggaaacggt aatcagaaaa ttgttcgcgt gtcttataca tggaagttgg
       61 cggatgttcc tgcatgggtg gacaaaaaag cgttctccag cgtcaagggt atgaatgaac
      121 ctgaagacgg catgattaat cttgttaaaa ccagcaatgg ctggaaagcg atctaagggg
      181 ctatcatgga aaacaatacc atcaagtgtc cattttgcct gaaagaaagc caaaggggtg
      241 tccatgtctg caccggctgc atggcgacgg ttctgtatgg aacctacccc ggctggtatg
      301 ctttcgtggt tatattattg accttcggcc tgtctatact gatcggaatg tctacaggta
      361 tggcgggagc tactatcagc ctaccaataa ttgcggctgt tgggtttgtc gcaggtaagg
      421 caattttctc tgataatgtt gtgtttaggc gaaagatgta aaacccttac cgtaaatgca
      481 aagaaaccat atctgttgaa ctaagtgtgg tttttttgca taacaaagta ttttatattc
      541 caaaaaaaca tataaaacat gttgcctgtc aggcaacaac tatctattat caaatcatgg
      601 acgaacgaac tcgtttgtct ttgctcttta aaaacctcta ggatcggtaa aggactgagt
      661 gaaactttca gtcaaccatt tctccaatac gagacgtggc ccatgtccct cttcattgtg
      721 atcgtaactt aatgcgaaat gtatattgca atcgtctatc tacgatacgg cattgtttaa
      781 tgctgaaatg gcggggtatc caatcagttg tgttggatgg gtgttggctg gctttgtcta
      841 cggtgtgcaa cacgatccac aagacatttt ggcggggatg caagttgcat cgtgttgtta
      901 aaatgagagg tttttatgaa agatgtttta gacactttgc gccaagtgcg tgaaaagagc
      961 aaacacactg tagacaatag ctctttgaaa aaactggatg atgcgattct gacccttgaa
     1021 aagagaatcg aggagaattc taaaggccac acagccttag attatctttc ttatatcgcg
     1081 aagatctttg ctttaattaa acttctgagc ggtgattctg attttcattg atatctacct
     1141 actaagggga tttgttatgt atccatcttc attttttgaa cgagcagcca gccatgtcaa
     1201 tcccattatc tgaggtgcac tatgttagat attggttcaa ccattaaact atgccgtgaa
     1261 gcacgtaagc tcaccttaca agaactttca gatagaacgg atttaacgaa atcttatcta
     1321 tctcgtatag agaacaatca aagagatcct actattacag cgttagaaag aatatcatta
     1381 gcgctacata tccctctaaa tataatcatc cttctttctg aaagtgaaga gacaaatgat
     1441 gagttttctg acatcaataa tatgctcaaa aaaactataa tggatacatt agttaatgca
     1501 taaaaattgg aattattcaa ataaaccact tgctaattta gagtc
//
LOCUS       DAOFQD010000301         1544 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.309, whole genome shotgun sequence.
ACCESSION   DAOFQD010000301 DAOFQD010000000
VERSION     DAOFQD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.309"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1456)
                     /locus_tag="QBF46_004876"
     CDS             complement(<1..1456)
                     /locus_tag="QBF46_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDQ6676947.1"
                     /translation="MAIFHLDFKIVKRSEGRSSVAKAAYHARTRITDERTGDTYDYSR
                     RTDLYGHFILAPANAPEHIVKDSTALWNEVERVERQNNGQTSRYFDVAIPAELNNDDK
                     KRLVLEYCQKNFVDKGMIADIAFHDLDSDNPHAHVMLTLKTIGPEGFGKKERSWNDRK
                     MSIFWRESWASMANSFLAAAGSSERIDHRSLQVQHEEALEKAAVALDNEEQALWLAKA
                     AETNRPAMKRIHSAKWRNKAAQEQRAAEQAVRDAAKQEAVEVYKTFSELDLEIVVDVR
                     SFTITHLAEPEEIVLPETRSSSSTNEEQNQEKRNLSEISSEKQRPLLVAPAPDTRMRG
                     VKSYRDKTKVSKVVAGKKPSVSISGSGTNTILKISSSQNPNRASRSAPERVKRKQAIP
                     RQDNIFKRFTTLVIDFFKQKFVWAKANKSQIDFISEEHDKRIAENFVFDKVLGHHVSR
                     AEYEKQAKFNSDTYKPTPDEINRFPSRQNSEHNNA"
BASE COUNT          414 a          312 c          313 g          505 t
ORIGIN      
        1 tggcattgtt gtgctcgcta ttctgacgac ttgggaaacg gttgatctca tccggtgttg
       61 gtttgtaagt atcactgtta aacttagctt gtttctcata ttcagcacga gaaacatgat
      121 gacctaatac tttatcaaag acaaagtttt cagcaatacg tttatcatgt tcttcactga
      181 taaaatcaat ttgggatttg tttgctttag cccatacaaa cttctgcttg aaaaaatcaa
      241 tgacaagagt tgtgaagcgt ttaaaaatat tatcctgacg tggaatagcc tgttttctct
      301 taacacgttc aggggcgctt ctggatgccc tgtttgggtt ttgagaggag gatattttca
      361 ggatagtatt tgttccagaa ccagaaatac tgactgatgg tttcttacct gcaacgacct
      421 tactgacttt tgttttatca cggtaagatt taacgccacg catacgagta tcaggagctg
      481 gtgctaccag taaaggtctt tgtttttctg acgaaatctc ggagagatta cgtttttctt
      541 gattttgttc ttcatttgta gaagagctgg atcgcgtttc tggtaaaaca atttcttctg
      601 gttcagcaag atgtgtaatc gtaaagcttc tgacatcaac gacgatttca aggtcaagtt
      661 cgctgaatgt tttgtagact tcaactgctt cctgcttggc agcatcacga acagcctgtt
      721 ctgcggctct ttgttcctga gcagctttat tacgccattt ggcactgtgg atacgcttca
      781 ttgcaggacg atttgtttca gcggctttag caagccatag tgcttgttct tcattatcta
      841 aagctactgc tgctttttct aacgcttcct cgtgctggac ttggagagaa cgatgatcga
      901 tacgttctga tgaaccagca gcagcgagga agctgttagc catagatgcc catgattcac
      961 gccaaaaaat agacattttt ctgtcgttcc agcttctttc ctttttgcca aaaccttccg
     1021 ggcctatggt ttttaaggtt aacataacgt gagcatgagg gttatcacta tcaagatcat
     1081 ggaaagcgat gtcagcaatc atgcctttat caacgaaatt tttctgacaa tactcaagga
     1141 caagtctttt cttgtcgtca ttgtttaact cggcaggaat agcaacatcg aaataacggg
     1201 atgtttgacc attgttctgg cgctcaactc tttcaacttc attccacaat gctgtagagt
     1261 cttttacgat gtgttctggt gcgttagctg gagctaatat gaaatgccca tataaatcag
     1321 tacggcgact gtaatcatac gtatcacctg ttctttcatc tgttatgcgt gtacgagcat
     1381 gataggcagc cttcgcaacg gaagacctgc cttctgaacg tttcacaatt ttaaaatcaa
     1441 gatgaaaaat agccatacaa taaaaagaac ctcaatataa ttttaaataa caaaccaaaa
     1501 tataaatctc caatgcgact atgctggttt tagtttttcg caag
//
LOCUS       DAOFQD010000302         1530 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.310, whole genome shotgun sequence.
ACCESSION   DAOFQD010000302 DAOFQD010000000
VERSION     DAOFQD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.310"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1459
                     /gene="ltrA"
                     /locus_tag="QBF46_004877"
     CDS             <1..1459
                     /gene="ltrA"
                     /locus_tag="QBF46_004877"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDQ6676948.1"
                     /translation="QRLLRLITQPEWLAEAARITLSSKGAHTPGVDGVNKTMLQARLA
                     VELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALRDRIVQRAMLMAMEPIWE
                     SDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEGDLSSYFDTVHHRLLMKA
                     VRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVISPLLSNIMLNEFDQYLH
                     ERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCRYADDFVLIVKGTKAQAE
                     AIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIRKRSRYGEMRVVSTIPQE
                     KARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQFVDFKAKVFSYIDRVVFW
                     KLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNHGKLSGEILYRLVGQGKK
                     LFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          391 a          379 c          442 g          318 t
ORIGIN      
        1 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
       61 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      121 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      181 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      241 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
      301 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
      361 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
      421 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
      481 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
      541 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
      601 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
      661 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
      721 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
      781 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
      841 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
      901 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
      961 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1021 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1081 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1141 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1201 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1261 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     1321 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     1381 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     1441 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     1501 ggaggagagg cagggaaata gtccgactac
//
LOCUS       DAOFQD010000303         1524 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.311, whole genome shotgun sequence.
ACCESSION   DAOFQD010000303 DAOFQD010000000
VERSION     DAOFQD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.311"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            1..1524
                     /locus_tag="QBF46_004878"
                     /pseudo
     CDS             1..1524
                     /locus_tag="QBF46_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          362 a          431 c          470 g          261 t
ORIGIN      
        1 tcggctggag tgagcgggtg ccgggggggg aatgaggtgc tgcctgcccc attgccgccg
       61 taccgggtgc tgaccggcct ggtggaccgc ttcgggcgca cgcaaacctt ccaccgtgag
      121 gccgcaggcg aatttaccgg agaaatcacc ggcgtgaccg atggtgcagg ccgtcagttc
      181 cgcctggtgc tgaccacgca ggcgcaacgg gcagaaaacg cccggcagca ggccatcgcc
      241 gcaggaacga aaggatcgga tatcccggat tcactgccgg actatacgga gtatggccgg
      301 gataacggta tccgcctgtc aggggtgtgg ctgacgcacg acccggaata cccggagaat
      361 ctgcccgccg cccccctggt acgctacgac tggacggcac gcggtgagct ggcggcggtg
      421 tatgaccgca gcggtactca gatgcgccac tttacttacg atgataaata ccggggccgg
      481 atggtggcgc accgttacgc aggcaggccg gagatgcgtt accgctacga cgacataggt
      541 cgcgtgacgg agcagtttaa tccggcaggc ttaagctacg cctatcagta tgagaaaaac
      601 cgtatcacca tcaccgacag cctgaaccgg cgggaggtgc tgcacacgga aggtgaagcc
      661 gggctgaaac gtgtggtgaa aaaggaattc gcggatggca gcatcacccg aagtaaattt
      721 gactatatgg ggcgactcca gtcacagacg gatgcagcag gccggacaac agaatacagt
      781 ccgaatgtgg tgacgggact catcacgtgc atcaccacgc cggacggcag gaaatcggaa
      841 ttttactata acaatcaaaa ccagttaacc tcagccaccg ggcctgacgg actggaaatg
      901 cgccggaaat atgatgagta cgggcgtctg acgcaggaaa cagcacgcaa cggcgatgtc
      961 acccgctaca gttatgataa tccacacagt gaattaccct ctgtaacaga agatgcgacg
     1021 ggtagccgca agcagatgac gtggagccgc tacgggcagt tacagacctt caccgactgc
     1081 tcaggctatg aaacccgtta tgaatatgac cgcttcgggc aaatgacggc ggttcaccgt
     1141 gaggaaagca tcagccttta ccgcagttac gacaaccgtg gccagttaat ctcggtgaaa
     1201 gatacgcagg gccgtgaaac gcagtatgag tacaacgccg caggcgaccc gaccgccatc
     1261 attgccccgg acggcaaccg gagtgagaca cagtacgatg gctaccgttc tcgttggtca
     1321 ggctgatgac acgtcccgct gcatcgtatt ccatgctgcg cgtcagccca ccctgtgcgg
     1381 tgctgacggc ctttccccac gcatccagaa tttagctggg atgtgcttga ccgcctgata
     1441 cagcagcgcg gctttgacgg gcggacgcag cgttaccgtt atgacctgac cgggaaactc
     1501 acgcagagcg aggatgaggg gctt
//
LOCUS       DAOFQD010000304         1516 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.312, whole genome shotgun sequence.
ACCESSION   DAOFQD010000304 DAOFQD010000000
VERSION     DAOFQD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.312"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..1418)
                     /locus_tag="QBF46_004879"
     CDS             complement(<1..1418)
                     /locus_tag="QBF46_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDQ6676949.1"
                     /translation="MAIFHLDFKIVKRSEGRSSVAKAAYHARTRITDERTGDTYDYSR
                     RTDLYGHFILAPANAPEYIVKDSTALWNEVERVERQQNGQTSRYFDVAIPAELNNDDK
                     KRLVLEYCQKNFVDKGMIADIAFHDLDSDNPHAHVMLTLKTIGPEGFGKKERSWNDRK
                     MSVLWRESWASMANSYLAAAGSSERIDHRSLQDQHEEALEKAAVALDNEEKALWLAKA
                     AETNRPPMKRIHSAKWRSKAAQEQRAAEQAVRDAAKQEAVEVYKTFSELDLEIVVDVR
                     SFTITHLAEPEEIVLPKTSSSSSANEMQRPVLVAPPPHTRMRGVKSYRDKSKVSKVVA
                     GKKPSVSISDSGKNTILKTSSSQALNRASRSAPERVKRKQAIPRQDNIFKRFTTLVID
                     FFKQKFVWAKANKSQIDLISEEHDKRIAENYVFDEVLGRHISRAEYEKKSKFNQDDYK
                     PTPDEINRFPSRQNSEHNNANR"
BASE COUNT          411 a          310 c          304 g          491 t
ORIGIN      
        1 tccctattgg cattgttgtg ctcgctattc tgacgacttg ggaaacggtt gatctcatcc
       61 ggtgttggtt tatagtcatc ttggttaaac ttggattttt tctcatattc agcgcgagag
      121 atatgacggc ctaatacctc gtcaaagacg tagttttcag caatacgttt atcatgttct
      181 tcactgataa gatcaatttg ggatttgttt gctttagccc atacaaactt ctgcttgaaa
      241 aaatcaatta caagagttgt gaagcgttta aaaatattat cctgacgtgg gatagcctgt
      301 tttctcttaa cacgttcagg ggcgcttctg gatgccctgt ttagggcttg agaggaggat
      361 gttttcagaa tagtattttt tccagaatca gaaatactga ctgatggttt cttaccagca
      421 acaaccttac tgactttgga tttatcacga taggatttaa cacctcgcat acgagtatga
      481 ggaggtggtg ctacaagtac aggtctttgc atttcattgg cagaagaact ggagcttgtt
      541 tttggtaata caatttcttc tggttcagca agatgtgtaa tcgtaaagct tctgacatca
      601 acgacgattt caagatcaag ttcgctgaat gttttgtaga cttcaacggc ttcttgcttg
      661 gcagcatcac gaacagcctg ttctgctgct ctttgctctt gtgctgcttt actacgccat
      721 ttggcactat ggatacgctt cattggagga cggtttgttt cagcggcttt agcaagccat
      781 agcgcttttt cttcattatc taaagctacc gccgcttttt ctaatgcctc ttcatgctgg
      841 tcctgaagag aacgatggtc aatacgttct gacgagccag cggcagcgag gtagctgtta
      901 gccatcgatg cccatgattc acgccataaa acagacattt ttctgtcgtt ccagcttctt
      961 tcttttttac caaacccttc cgggccaatg gtttttaatg tcaacataac gtgagcatga
     1021 ggattatcac tatcaagatc atgaaaagcg atgtcggcaa tcatgccttt atcaacgaaa
     1081 tttttctgac aatactcaag gacaagtctt ttcttgtcgt cattgtttaa ctcggcagga
     1141 atagcaacat cgaaataacg ggatgtttga ccattttgtt gacgctcgac tctttcaact
     1201 tcattccata aggctgtaga gtcttttacg atatattctg gagcattagc aggagctaag
     1261 atgaaatgcc catataaatc agtacggcgg ctataatcat acgtatcacc tgttctttca
     1321 tctgttatac gtgtacgagc atgataggca gctttcgcaa cggaagatct gccttctgaa
     1381 cgtttcacaa ttttaaaatc aagatgaaaa atagccatac aataaaaata acctcaatat
     1441 aaatttaaat atcaaaccaa aaaataaatc tccaatgcga ctatgctggt tttagttttt
     1501 cgcaagaaaa actttg
//
LOCUS       DAOFQD010000305         1512 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.313, whole genome shotgun sequence.
ACCESSION   DAOFQD010000305 DAOFQD010000000
VERSION     DAOFQD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.313"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..354)
                     /locus_tag="QBF46_004880"
     CDS             complement(<1..354)
                     /locus_tag="QBF46_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDQ6676950.1"
                     /translation="MRKFLAIAGLTVLLSGCGEKGDFEKAINAKISHSKLCYSLQDND
                     IVFNKGFPIRVNHGYRSAGYSASDEILKGLANQGLLDVSQQANGFSSVDVLEVTDKGQ
                     AVEFWDRKDGACVGHR"
     gene            complement(360..935)
                     /locus_tag="QBF46_004881"
     CDS             complement(360..935)
                     /locus_tag="QBF46_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676951.1"
                     /translation="MKLRIVMPLVLSAAALTGCQQLNDGVNTVNGAISSSLSSVGKAL
                     GGTATPQSLGPVSSVSGLGAVCKDYEENAMAAEKQWTGKRISITNATVLEVTKGRSAS
                     DMMTGMPKTAPNYYLLFKTADTSYCGGMVWLDYYSGIDDTVLTYKKGQKVSVTGTVNN
                     FEVRGFATVNNADMPVRLVVLKNGTISHKKG"
BASE COUNT          418 a          353 c          302 g          439 t
ORIGIN      
        1 acgatgacca acacaggcac catctttacg atcccaaaac tcaaccgctt ggcctttgtc
       61 tgttacttcc agaacatcga cactactgaa accattagct tgttgagaaa catcaagcag
      121 cccctgatta gccagacctt tcaatatttc atcactggca ctataccccg ctgaacggta
      181 gccatggttg acccggatgg ggaagccctt attgaacaca atgtcattat cctgcaagga
      241 gtagcacagc tttgaatgtg aaatcttcgc attgatagct ttctcaaaat cacctttttc
      301 accgcagcct gataacaaca cggtaaggcc cgcaatggct aaaaatttac gcatggttct
      361 tatccctttt tgtgactgat agtgccgttt ttcagaacaa ccagacgaac tggcatatcg
      421 gcattgttga cggtagcgaa tccacggact tcgaaattgt ttacagttcc ggtgacgctg
      481 actttctggc ctttcttata agtaagaacg gtatcatcaa taccagagta gtaatccagc
      541 cataccatgc caccgcagta gcttgtatcg gctgttttaa agagcaggta atagttaggg
      601 gctgttttgg gcattcccgt catcatatcg ctggcacttc tgcccttcgt aacttccaga
      661 acagtggcat tggtgataga gatccttttg ccagtccact gtttctctgc tgccattgcg
      721 ttttcttcat agtctttgca gacagcacca aggccagata cagacgatac cgggccaagg
      781 ctttgaggtg tggcagttcc ccccagcgct ttacctactg aagacaacga agaactgatt
      841 gctccattaa ctgtattcac gccatcgttg agctgctggc atcctgttag tgcggcagca
      901 gacagtacca gtggcatcac tatccgtaat ttcatatcta tccccaatgt gtggaaactt
      961 tatgtcaaag acagccctac ccataggttg gtctctctgg gctgaaaaaa agcattaatc
     1021 aattgatagg attcaattta tttttctgtt cgtcatatcg tttgtatcga tcgaattcaa
     1081 atcatttgat agctataggc tatcatatat acacaatcga tcgatataaa caattaaaac
     1141 accgatcaat tgacacccat tcaatagatt caacctatca aaggaaattt attgatcttt
     1201 tatatcgatc aataagtggt gtaatgtgca cgaagagcca tgtcagtagc tgtttgcaca
     1261 tggatggaga tagaaaggta taggtcagaa caggtaatac cctgtcgacc attacgcaga
     1321 cagggattgt tagatctcag gtaacgtagc tatcttccga tatggtgttg caaaaacctg
     1381 tatgcaacgc atgttttaat atgctgtttc atatggaatg gttgctacca ttcagatttt
     1441 tcaatcatct tccaaagcac tcacaccgct tgccgggaaa ttgcttttca tattattcga
     1501 tatcttcgga ac
//
LOCUS       DAOFQD010000306         1483 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.314, whole genome shotgun sequence.
ACCESSION   DAOFQD010000306 DAOFQD010000000
VERSION     DAOFQD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.314"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(8..1366)
                     /locus_tag="QBF46_004882"
     CDS             complement(8..1366)
                     /locus_tag="QBF46_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDQ6676952.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSEKTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
BASE COUNT          491 a          332 c          238 g          422 t
ORIGIN      
        1 tgccgcttta tggtgactca caaaaattca ccacatccct cacaaccacc ttattacgcc
       61 cggtctcttt cgcctcgtaa agtgcgttat cagccagtcg atagatctca tttgggttta
      121 acgcattttc ctgagtcaca acagcgccaa tactaatcgt cactttttgt ggtatagcgt
      181 attcaggatt atcgccagtt aaacgctcaa cattttcccg aatcctttcc gctaaagctt
      241 tcgcacgttc agtatcgatg tccgtcagca agacgccaaa ctcttcgccg ccgacgcgcg
      301 ctaaaaggtc atctggtcgt atgcttttcc caatgatatt caccactgtt tttatcacct
      361 gatcgccaac cggatgcccc caggtgtcgt tgactttttt gaagtggtcg atatccataa
      421 tcatgacgca ataaggcgtt ttttgggctg atgctgattg aactgtcagt tcattaaaaa
      481 aataatttct gttaaatata ttggttaagg gatcgcgatg tgcaatgttc tttgttactc
      541 gtagcgcact gaatatatgg cacataaata ttaccataac ggttaactta ctgactactt
      601 caatagtgcg actaatatac caagtggaca cgctatacct gctcataaat aacagagaga
      661 tattgtacag tacggcacaa aaagcaatca ccgtcactcc attccagata tcatagcgta
      721 aaccattaaa gtatagatta accgaaagca aggttaacca catgaaaatc attatggtaa
      781 catatgaggc tttccaaacc acctgaccat tttcattggt taactcggca atgtgtaaat
      841 tgtagctctc ataatgacta gaaagtatgt gagcaactat cggcccccca aaaacaaaaa
      901 acaaacttat gcacaaagtc atttttttgg aaaataaatt gcgctgtttt gttttttcac
      961 taactttatt caccaatgcc agaaatatta atatgcacaa actcatctga cgaaacaaat
     1021 aatagattgc aatgtcattt tggacaacac tgcttgtcaa acgcccctca ataatttgct
     1081 gaatgataat gacggtttta acaaagtaaa tcaaacaact cagaaaagcc aaactaagta
     1141 cacagtttga aaattgccta cggtcataaa tgaaatacaa ctgcataaaa ataaatgcaa
     1201 cagtatcgat aaaaaataaa gcaatcatta ccatcagata ggttgatcgt ggaacaactt
     1261 cctcaacgcc tggctgatta aaccaactat tcaccaaaac caaagcaagc ccaaacaata
     1321 agctcactaa tacagtacta ctaattctca aatagtcttt ttccataaag ctacaccaat
     1381 ctgtaggttg tagatccctt tctattatta atagatataa gaactgtact gttgtcaata
     1441 tgaaattccg cccacaaaca ataccgatga tgatagttgc tga
//
LOCUS       DAOFQD010000307         1457 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.315, whole genome shotgun sequence.
ACCESSION   DAOFQD010000307 DAOFQD010000000
VERSION     DAOFQD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.315"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..311
                     /locus_tag="QBF46_004883"
     CDS             <1..311
                     /locus_tag="QBF46_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676953.1"
                     /translation="SEFAFAFDLLKDMPRLWRRIFLLIRVDGLNASCVSEILLIPSKE
                     DAVRRWLMQAEAFIAAHLEEAGVSKNGNDWLQGVDWLALSVTRSEAASLWSKEANHEE
                     "
     gene            301..741
                     /gene="trx-GI"
                     /locus_tag="QBF46_004884"
     CDS             301..741
                     /gene="trx-GI"
                     /locus_tag="QBF46_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance system thioredoxin Trx-GI"
                     /protein_id="HDQ6676954.1"
                     /translation="MKNDLHLVCPHCQSINRVPTAKLSEHPNCGRCQQPLFTGEPIEL
                     TTATFSRHVERSDLPLLVDFWAPWCGPCKMMAPQFQQAAHQLEPRIRLAKVNTEAEPH
                     LATQFGIRSIPTLALFQGGREIARQAGVMGAQDIVRWTSTQVGR"
     gene            745..>1457
                     /gene="kefB-GI"
                     /locus_tag="QBF46_004885"
     CDS             745..>1457
                     /gene="kefB-GI"
                     /locus_tag="QBF46_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance system K+/H+ antiporter KefB-GI"
                     /protein_id="HDQ6676955.1"
                     /translation="MQGLLGTTLILLAACSLAAMATAAFRVPALLGYLSVGVVLGPSV
                     IGVIAPGETLSFLSELGVALLLFMVGLEFSLGDFWLARRTVLMAGALQMIAVAPPLIL
                     LLMWLGQPGQSAALLGTAAAMSSTALVSRQLADQGELTTRHGRSAIAVLVFQDLASVP
                     LLALLAIWARGESPKIEHVLLEVFGVLLLFAAAALASRRLLHGLLGWVARRGHEESFV
                     LVSLCVVVAAAAAAHAVGVS"
BASE COUNT          221 a          448 c          494 g          294 t
ORIGIN      
        1 cgagcgagtt cgcttttgca tttgacctgc tgaaggacat gccgcgtttg tggcggcgca
       61 tttttctgct tatccgtgtg gacgggctga atgccagctg cgtctctgaa atcctgctga
      121 ttcctagtaa ggaagatgct gtccgaaggt ggctcatgca ggccgaagca ttcatcgctg
      181 ctcatctgga agaggccgga gtaagcaaaa acgggaacga ttggctccaa ggcgttgatt
      241 ggttggcatt gtctgtgacc cgaagcgagg cagcctcact gtggtccaag gaggcgaacc
      301 atgaagaatg atcttcacct cgtctgcccg cactgccagt ccatcaaccg cgtaccgact
      361 gcgaagctat cggaacatcc caactgtggc cgctgccagc agcccttgtt cacgggcgag
      421 cccatcgagt tgaccacggc gacgttctcg cgccacgtgg agcgcagcga tctgccgctg
      481 ctggtcgatt tctgggcgcc atggtgcggg ccgtgcaaga tgatggcgcc acagttccag
      541 caagcggcgc accagctcga accgaggatc cggctggcga aggtgaatac cgaggctgag
      601 ccccacctgg ccacgcagtt cggtatccgc agcatcccga cgctcgccct gttccagggt
      661 gggcgggaga tcgcccgtca ggccggcgtg atgggcgcgc aggacatcgt gcgctggacg
      721 tcgacgcaag tgggacgctg accgatgcag ggcttgctcg gcactacact aatcctgctg
      781 gcggcttgca gcctggcggc gatggcgacg gcggcgttca gagtgcccgc cttgctgggg
      841 tatctctctg tcggcgttgt actgggcccc tcagtcatcg gcgtgatcgc gccgggggag
      901 acgctgagct ttctgtccga gctgggcgtg gcgctgctgt tgttcatggt cggactggaa
      961 ttctccctcg gagacttctg gctcgcccgc aggacggtgc tgatggcggg cgctctgcag
     1021 atgatcgccg tagccccgcc gctgatcctg ctattgatgt ggctggggca gcccggccag
     1081 agcgccgcgc tgctcggcac tgcggcggcc atgtcgtcca cggcgctggt cagccgacaa
     1141 ctggccgacc aaggtgagct caccacccgc cacggacgca gtgccatcgc cgtgctggtc
     1201 tttcaggacc tggccagcgt gcccctgctg gccttgctgg cgatctgggc gcgcggcgag
     1261 tcgccgaaga tcgagcatgt gctgctcgaa gtattcggtg tgctgctgct gttcgcggca
     1321 gcggccctcg cctcgcgccg tctgttgcat ggcctgctgg gctgggtggc acggcggggc
     1381 cacgaggaat cgttcgtgct cgtctccttg tgcgtggtgg tggctgccgc cgccgctgcg
     1441 cacgcggtcg gcgtatc
//
LOCUS       DAOFQD010000308         1454 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.316, whole genome shotgun sequence.
ACCESSION   DAOFQD010000308 DAOFQD010000000
VERSION     DAOFQD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.316"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>1454
                     /locus_tag="QBF46_004886"
     CDS             <1..>1454
                     /locus_tag="QBF46_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDQ6676956.1"
                     /translation="FLNSNDNSLSLLSWEMIFKDIGKTKNKRNEEHLRLLEGLTAVHI
                     TNIAYAKAEIFPVENISEESEPDNISSWIFKFIPKFDETTEELSKSLSIDSPSVRLEK
                     IINSSENNDKVSWSLVDNNDFSRVDEEILLEFYGYETDQDNQEIYSFISKKPLPEWRN
                     FYIVPDSIEGTLRQITRHANTLDMLENHIELMNVITSPNSNLTVSNEDIIAKDIMDSL
                     DESKQRVFTKVLSTLPMNLVQGPPGVGKTHLVKAISKFIFKEEPNSRILFTAQSHATV
                     QHLYHEVVKDLLTENKHTNDPIIIRCNKISGEDNIILNSADETGLEYLRKFVESPLYK
                     NSSNQSLKSNISSLLKAPPVKRYPLINQLIKSASLIFATTNSDYVERMIKERAQFDWS
                     IMEESGKVTGIELISPLLLSYRRLMIGDHNQLPPYRSIELRNILTNNTKLKNTFEEVD
                     SISNFKLKNELMRNSHFSSITDDQAREIGLRAAK"
BASE COUNT          573 a          241 c          237 g          403 t
ORIGIN      
        1 aatttttgaa tagtaatgat aactcattaa gcctactgtc ttgggaaatg attttcaaag
       61 atataggtaa aactaaaaac aaaagaaatg aagaacatct tcgattattg gagggcctaa
      121 ctgccgttca tattacaaat atcgcatatg caaaagcaga aattttccct gtagaaaaca
      181 tcagtgaaga aagcgaacca gataatatta gctcatggat ctttaaattc attcccaaat
      241 ttgacgaaac aacggaggaa ctgtctaagt cgctaagtat cgactcacca tctgtcaggc
      301 ttgaaaagat tataaatagt agtgaaaaca acgataaggt atcctggagt ttagtagaca
      361 ataatgattt cagtagagtt gacgaagaga ttcttttaga gttttatggc tatgaaacag
      421 atcaagacaa tcaagaaata tatagcttca tctctaaaaa acctcttcca gaatggagga
      481 atttttatat tgttcccgac tcgattgaag gaactctgcg ccaaataaca agacatgcaa
      541 atacattgga tatgcttgaa aatcatatcg aattgatgaa tgtaattact tcccctaatt
      601 caaatctcac agtaagcaat gaagatataa ttgcaaaaga tataatggac tcattggatg
      661 aatctaaaca aagagttttt acaaaagtat tatcaacatt gccaatgaac cttgtacaag
      721 gtccaccggg agtaggtaaa actcacttag tcaaagcaat ttctaaattt atctttaaag
      781 aagaacctaa tagcaggata ctctttaccg cacagagtca tgcaaccgtt caacatctat
      841 atcacgaagt tgtcaaagat ttacttactg aaaacaaaca tacaaatgat cccataatta
      901 ttagatgcaa caaaatatct ggcgaagata atataatatt aaacagtgcc gatgaaactg
      961 gattagaata tttgcggaag ttcgtagaaa gccctttata caaaaactct tccaatcaaa
     1021 gtttaaaaag taatatttcc agtttgctta aagctcctcc agtaaagaga tacccactaa
     1081 ttaatcaact aattaaatca gcaagcttaa tatttgctac aactaattca gattatgtag
     1141 agagaatgat aaaagaaaga gcacaatttg attggtcgat catggaagaa agtggaaagg
     1201 ttacaggtat agaattaata agcccacttt tattatccta ccgacgatta atgatcggtg
     1261 atcacaatca attgccaccg tatcgttcaa ttgagttaag aaacatttta accaataata
     1321 caaaacttaa aaacaccttt gaagaagttg acagcataag taattttaaa ttaaaaaatg
     1381 aactaatgcg taatagtcac ttttcatcaa taaccgatga tcaagcaaga gaaataggat
     1441 tacgggcagc caaa
//
LOCUS       DAOFQD010000309         1444 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.317, whole genome shotgun sequence.
ACCESSION   DAOFQD010000309 DAOFQD010000000
VERSION     DAOFQD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.317"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            183..1205
                     /locus_tag="QBF46_004887"
     CDS             183..1205
                     /locus_tag="QBF46_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5708 family transposase"
                     /protein_id="HDQ6676957.1"
                     /translation="MTITTVGIDLAKNVFAVHCIDQNGKAVLVKPKVSRAALSELIAG
                     LPPCVIGMEACSGAHYWARLFRQYGHDVRLMAPKFVSPYRMAGKTGKNDAADAIAICE
                     AVQRPHMRFVPVKDESQQAMQCLHRTRQGFIQERTATYNRLRGLISEFGVIAPQSTDA
                     LRHVVSDQKETLPVQVRLCIDDLLTHVTNIEEKIAEYDRVLSRVAKTDHRSQQLMELK
                     GVGPTTASALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDAYLRT
                     LLVQGARSVMVGAENRTDPFSRWVCSLIGRRGYWRAVVAIAAKNARLCWASLHYGDDF
                     RLYSTT"
BASE COUNT          391 a          324 c          371 g          358 t
ORIGIN      
        1 aaaaccatta acggacagcc ggatcatttc gtacatccct tacggtcagg ggttcgcgct
       61 caatggaacg aaatcacccg ttagcttaaa acgcttaacg aatggaaggg acttccccta
      121 gatatgcgcc tgattaactt tcgtgcaatc ataggataaa cattgaccca gagggattcg
      181 ttatgaccat caccactgtc ggtatcgatc ttgctaaaaa tgtgtttgct gttcactgca
      241 ttgaccaaaa tggtaaagct gttctggtta aacccaaggt gtcgcgtgca gcactttctg
      301 aactcatcgc aggtttacct ccctgtgtca ttggaatgga agcctgctcg ggtgcccact
      361 attgggcaag attattcagg cagtatggcc atgatgtccg gttgatggct ccgaaatttg
      421 tctcacccta tcgcatggcg ggtaagaccg ggaaaaatga tgcagctgat gcaatagcta
      481 tttgtgaggc agtgcagcgg ccgcatatgc gctttgtacc agtcaaagac gaaagccagc
      541 aggcaatgca atgcttacat cggacaagac agggatttat ccaggaaaga acagcaacgt
      601 ataaccgtct tcggggcctg atatctgaat tcggggttat tgccccacaa agcactgatg
      661 ctctgcgtca tgttgtttct gaccagaagg aaactctgcc agtacaggtt aggctgtgca
      721 ttgatgatct attgacacat gtcactaata ttgaagagaa aatcgccgaa tatgacaggg
      781 tattatctcg tgtggccaaa acagatcatc gcagccagca gttaatggaa ctgaaaggtg
      841 ttgggcccac cacagcgagt gcgctggtcg ccagtattgg caatgcccat gattttaaga
      901 acgggcgcca actggcagcc tggttggggc taacgccatc acaatacagt agtggcggaa
      961 aatcaaagct tggcaggata acaaaagcgg gtgatgccta tctgcgaacc ctactggtcc
     1021 agggagcccg ttctgtaatg gtaggggcag agaatagaac ggacccgttt agccgttggg
     1081 tctgctcgtt gattggtagg cggggatact ggcgtgctgt cgttgcaatc gccgccaaga
     1141 atgcgaggtt gtgctgggca tcattacatt acggcgatga cttcagactg tattcaacaa
     1201 catgaaaaat ttaactgagt agtaactgaa ctgtaaattg ttgatggtaa agggttagac
     1261 ccccgtgaga agtatctgca tatcctactg gatatcacta tccgattaac gaaggaggat
     1321 ctcacgagcg tctttcatca gggtccgaat cactagcgat tcagcatgac cgtttatagt
     1381 agcgcagtct tatcccttgc ctttcattga tgaacagaca gaaattaaaa accggcgttg
     1441 actg
//
LOCUS       DAOFQD010000310         1437 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.318, whole genome shotgun sequence.
ACCESSION   DAOFQD010000310 DAOFQD010000000
VERSION     DAOFQD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.318"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..321)
                     /locus_tag="QBF46_004888"
     CDS             complement(<1..321)
                     /locus_tag="QBF46_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676958.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPGGKCIITGDFQKELAYLQNVILYNVSSLRLDFLGYNAQIIQRSD
                     NTCEL"
     gene            complement(343..882)
                     /locus_tag="QBF46_004889"
     CDS             complement(343..882)
                     /locus_tag="QBF46_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676959.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYHGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1138..1437)
                     /gene="tdcR"
                     /locus_tag="QBF46_004890"
     CDS             complement(1138..1437)
                     /gene="tdcR"
                     /locus_tag="QBF46_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDQ6676960.1"
                     /translation="MVNTKKGLRPYFKQLPDNYQAKFELNLMSKFSNFIINKPFSVIN
                     NAACHIFSRYLLENKHLFYQYFKISNTCIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          452 a          249 c          258 g          478 t
ORIGIN      
        1 aagttcacaa gtattgtccg atcgttgaat aatttgggcg ttataaccta aaaaatccag
       61 acgaagagac gagacattat aaagaattac attttgtaag tacgctaatt ctttttgaaa
      121 atcaccagtg ataatgcatt ttccaccagg ttgtaacgtg ttaagtaaag tgaccatttc
      181 aatagaggtc tgttttagtt tttctatacc tggtgctagt gcttcgaggt tatttcctgt
      241 tgggctttca ggaaggtcat tcatttttcg acggactaaa tcactactaa tatcattacc
      301 aattgatgaa acagggaaca ttattttatc ctttataaat aatcaataaa aatcattatt
      361 aaaatgatcg ggtgaaaaaa agagaatttg ccaacaatct gaagtaatgc tggttttatt
      421 aaagtgcaca aggggtgtta agatggcttc ttttcgggtg aattttgcac aatacttaga
      481 aatcgtctcc ttaatacatt ccagctcttt tttattgacg gctgagcaag tacattgttc
      541 accaaaaggt gcaaaagtgc ctacatctaa tgtttgtatg atataatcat catgttctcc
      601 tgtagaaata tctataatga atggactcat attttccggt tggattcgga tcatgacaga
      661 aggaacatcg ccatgataga aattcaatgc agttattgca gataatactc tccgcagttc
      721 attacttata ctgtaaattt tattaacgtc attggtggag acaatttctg caaactttct
      781 ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt gcattggcgg
      841 gattgaaggg tgaaaaatat gcgcaattgg gaagcctttc attgatgcct gccttgtcat
      901 catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac tatcaaaaaa
      961 cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac gccgatcaaa
     1021 agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga tcagcaatta
     1081 ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgagggga gaagaattta
     1141 tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt tgacgttaat
     1201 caattgttcg agatgatcaa tacatgtatt cgaaatttta aaatattgat aaaataaatg
     1261 tttgttctcc aacaaatagc gtgaaaaaat gtgacatgcc gcattattta tcactgaaaa
     1321 tggtttattt ataataaaat ttgagaactt gctcattaaa tttaactcaa attttgcctg
     1381 gtaattatcc ggtaattgct tgaaatatgg tctcagcccc ttttttgtat taaccac
//
LOCUS       DAOFQD010000311         1402 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.319, whole genome shotgun sequence.
ACCESSION   DAOFQD010000311 DAOFQD010000000
VERSION     DAOFQD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.319"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            15..1067
                     /locus_tag="QBF46_004891"
     CDS             15..1067
                     /locus_tag="QBF46_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDQ6676961.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICVCLIGLIATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT          324 a          307 c          320 g          451 t
ORIGIN      
        1 ctgaggagtt taaagtgaac atatatatcg ggtggctttt caaattaatc cctttgatta
       61 tgggcttaat ttgtatcgcg ttaggtggct ttgtgctgga aagttcaggg caaagcgagt
      121 atttcgtcgc gggtcatgtg ctgatttctc tggcggccat atgcctggca ttattcacta
      181 ccgcatttat tatcatttcg cagctcacgc gcggcgttaa tacgttttac aatacattgt
      241 tccccattat tggctatgcg gggtcaatta tcaccatgat atggggttgg gcactgttag
      301 caggcaatga tgtgatggca gacgagtttg tcgccggcca tgttattttc ggcgttggta
      361 tgattgccgc ctgtgtatcg acggtggcag cgtcatccgg tcactttctg ctcattccca
      421 aaaatgcagc ggggagcaag agcgacggaa caccggtaca ggcttattct tcattaatcg
      481 gtaactgcct cattgccgtt cccgttttac tcaccctgct cggtttcatt tggtctatta
      541 cgctgttacg tagtgctgac ataactccgc attatgtcgc gggtcacgta ttgcttgggt
      601 taaccgcaat ctgtgtctgt ctaattggcc ttattgccac aattgtccat caaacacgta
      661 atacgttttc aactaaagaa cactggctgt ggtgttattg ggttattttt ctcggctcaa
      721 tcacggtact gcaggggata tacgtcttag tcagttccga tgcaagcgcc cgactggctc
      781 ccggcattat tcttatttgc ctcggaatga tctgttacag catattctca aaagtctggc
      841 tactggcact ggtatggaga cgtacctgtt cgttagccaa cagaataccg atgattcccg
      901 tcttcacctg cctgttttgc cttttcctgg catcgtttct tgcggaaatg gcgcagaccg
      961 acatgggata ttttattcct tcgcgggttt tggtcggttt gggagcggta tgctttacgt
     1021 tgttctcaat cgtttcaata ttagaagcag gttctgctaa aaaataattg caacgtaccg
     1081 gataaaacca gcgttgacca tttgcgtaac gctggttttt cttaggcatc atgaaataac
     1141 gcagcattaa tgcatagtgg ttaaggataa aaaaacaaca aagttactgt ctttttcagc
     1201 ctgttcatat tttataaaga tctggccact gggtgagttt tcaatcacct ttccttccac
     1261 cttatattaa gcctggaggg tttcagttct cgggctcatt agaaaataat cacaagatag
     1321 ccactatcaa aattagctca tttttaatgc ggctctaata attttcatct ttaggtaaca
     1381 ggtcaggacg acttcgctgc aa
//
LOCUS       DAOFQD010000312         1388 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.320, whole genome shotgun sequence.
ACCESSION   DAOFQD010000312 DAOFQD010000000
VERSION     DAOFQD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.320"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..341
                     /locus_tag="QBF46_004892"
     CDS             <1..341
                     /locus_tag="QBF46_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ6676962.1"
                     /translation="EVIFREYFYDTPPEGKYRCVGEDGLPAWADIPPPTREEQIASAE
                     TKKQQLINQANDYMNSRQWVGKAAIGRLKGEELAQYNLWLDYLDALELVDTSGAPDIE
                     WPTPPAVQAR"
     gene            complement(313..915)
                     /locus_tag="QBF46_004893"
     CDS             complement(313..915)
                     /locus_tag="QBF46_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ6676963.1"
                     /translation="MDNAVLNSEIIATKAGNITVYNYDGEIREYISTSNEYLAVGVGI
                     PAYSCLDSPGTYKAGYAICRSADFNSWEYVPDHRGEIVYNTETGEAKEITTPGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHGAAVDAAEAQRQSLIDTAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(915..>1388)
                     /locus_tag="QBF46_004894"
     CDS             complement(915..>1388)
                     /locus_tag="QBF46_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDQ6676964.1"
                     /translation="GEGSALPVGVPVPWPSATPPTGWLKCNGAAFSAEDYPNLAKAYP
                     TLKLPDLRGEFIRGWDGGKGVDSGRTLLSAQGDAIRNITGVVGYSSIGDSQGLLGYVS
                     GAFSASESKAPALSGASASGYQRSLYANFNASAVVPTANENRPRNIAFNYIVRAA"
BASE COUNT          359 a          342 c          333 g          354 t
ORIGIN      
        1 acgaagtaat tttcagggaa tatttctatg acacaccacc agaaggaaaa tacagatgtg
       61 ttggagaaga tggactgcct gcatgggcag atattcctcc accaacacgt gaagaacaaa
      121 ttgcatcagc cgaaactaaa aagcagcaat tgattaatca ggccaacgat tatatgaaca
      181 gtagacaatg ggttggtaaa gcggctattg gtcgtctgaa aggtgaggaa ctggcgcaat
      241 ataatttgtg gctggattat ctggacgcac tggagctggt cgatacttcc ggtgcaccag
      301 atattgaatg gcctacgcct ccggcagttc aggccagatg acatccggcg cggtgctggt
      361 atctgttgcc gtcaccgcgt caatgtaatc cagcacggcg ttaaggcggg ttgtttctgc
      421 ctgcgtcagc ttccgtccgg cctgtaattt cagttgaatc agactgatgg aggccattgc
      481 agtatcaatc agcgactggc gctgtgcttc tgctgcatct actgctgcgc cgtgctgtgc
      541 ctcagtatcg gtcacccatt tctcaccatc ccatttatcg tatggcgtta acggggcgat
      601 agtggttgta ttttcagggt aatcacccgg agttgtgatt tctttggctt ctcccgtttc
      661 ggtgttatag acgatttcac cgcgatggtc tggcacatat tcccatgagt taaaatctgc
      721 agaacggcag atagcataac cagccttata tgtgcctggt gaatctaaac aggaatatgc
      781 cgggatacca acaccaacgg caagatattc atttgaagtg gaaatatatt cccgtatttc
      841 accatcatag ttatagacgg taatattccc tgccttcgtg gcaataatct cgctatttaa
      901 tacagcgtta tccattatgc agccctcaca atataattaa aggcaatgtt acgtgggcgg
      961 ttctcatttg cggttgggac aacggctgaa gcattaaaat tcgcatacag ggagcgttga
     1021 tacccggatg ctgatgcacc acttaatgcg ggagcttttg actccgaagc cgaaaaggcc
     1081 cctgacacgt atcctaataa cccttgtgaa tcgccaattg aactgtagcc aaccaccccg
     1141 gtaatgtttc ggatagcatc tccttgtgct gaaagcagag ttcgcccact atccactccc
     1201 tttccaccat cccagccacg aataaactca ccgcgtaaat caggcaattt taacgtcggg
     1261 taagcctttg ccagattagg gtaatcttca gcagaaaaag ccgcaccgtt gcatttcagc
     1321 cagcctgttg gcggtgtggc tgaaggccat ggaacaggca caccaacagg cagcgcagag
     1381 ccttcccc
//
LOCUS       DAOFQD010000313         1375 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.321, whole genome shotgun sequence.
ACCESSION   DAOFQD010000313 DAOFQD010000000
VERSION     DAOFQD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.321"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..299
                     /locus_tag="QBF46_004895"
     CDS             <1..299
                     /locus_tag="QBF46_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ6676965.1"
                     /translation="GKYRCVGEDGLPAWADIPPPTREEQIASAETKKQQLINQANEYI
                     NSKQWPGKASIGRLKGEELAQYNLWLDYLDALELVDTSGAPDIEWSTPPAVQAR"
     gene            complement(271..864)
                     /locus_tag="QBF46_004896"
     CDS             complement(271..864)
                     /locus_tag="QBF46_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ6676966.1"
                     /translation="MIPVFDENGLATVPGDMRCFYYNAVTYEYTGWSDEYINTGVSIP
                     ACSTGIDPGENIPGKVAVFTGKGWSHEEDHRNETVYSTENGAAVTVDYIGAIKDGYVT
                     LSPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAGRKL
                     TQPENTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(864..>1375)
                     /locus_tag="QBF46_004897"
     CDS             complement(864..>1375)
                     /locus_tag="QBF46_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6676967.1"
                     /translation="AIGVPFFWPSAAMPNTVIDSWSSMVFLKFNGAKFSATDYPVLAK
                     VFPALALPDARGDFIRIWDDGRGIDVGRTLLSGQSHTIMDHAHNMELWTGDGLAAGSA
                     REGVNPGILATYGDGGIVKTDEPGLKVPSSLRALSSRSVKRYGEISGNVDTETRPRNI
                     AFNFLVRAK"
BASE COUNT          355 a          359 c          312 g          349 t
ORIGIN      
        1 aaggaaaata cagatgtgtc ggagaggatg gactgcctgc atgggcagat attcctccac
       61 caacacgtga agaacaaatt gcatcagccg aaactaaaaa gcagcaattg attaaccagg
      121 ccaacgaata cataaacagt aaacaatggc caggtaaagc atcaattggt cgtctgaaag
      181 gtgaggaact ggcgcaatat aatttgtggc tggattatct ggacgcactg gaactggtcg
      241 atacttccgg tgcgccagat attgaatggt ctacgcctcc ggcagttcag gccagatgac
      301 gtccggcgct gtgctggtat ctgttgccgt caccgcgtca atgtaatcca gcacagcgtt
      361 aagtcgggtg ttttctggct gcgtcagctt ccgcccagcc tgtaatttca gttgaatcag
      421 actgatggaa gccattgcag catcaatcag cgactggcgc tgtgcttctg ccgcttctac
      481 tgcggcgcta tgctgtgcct cggtatccgt cacccatttc tcaccatccc atttatcgta
      541 tggcgttaac ggtgaaagcg tgacataacc gtctttgatg gcaccgatat aatccactgt
      601 aacagctgcg ccattttcag ttgagtaaac ggtctcattg cgatggtctt cttcatggct
      661 ccatccctta cccgtaaata ctgccacttt tcccgggatg ttttcgcccg ggtcaatacc
      721 agtggaacag gcgggtatac ttacgccagt attaatatat tcatcagacc agccggtata
      781 ttcatacgtt actgcattat aataaaaaca acgcatatcg cccggcactg tagccagccc
      841 attttcatca aaaacaggta tcattattta gccctcacca gaaagttaaa tgcaatattt
      901 cgtggacggg tttctgtatc tacatttcca ctaatttcac cataacgttt aacactacga
      961 gagctaagag ctcgtagtga ggaaggcacc ttaagaccgg gttcgtccgt tttaactatt
     1021 cccccgtcac cgtatgtagc cagtattcct gggtttactc cttcccgtgc acttcctgcg
     1081 gcaagcccgt cccccgtcca caattccata ttgtgtgcat gatccataat tgtgtgtgat
     1141 tgccctgaaa gtagggtacg tccgacatca atcccgcgcc catcatccca gatacgaatg
     1201 aaatcacccc gtgcgtcagg taatgctagc gccggaaaca ctttcgccag cacagggtaa
     1261 tcagtggcag agaatttcgc gccgttgaac ttcaaaaaca ccatactgga ccaactgtcg
     1321 attacagtat ttggcattgc ggcggacggc cagaagaacg gaacgccaat agctg
//
LOCUS       DAOFQD010000314         1366 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.322, whole genome shotgun sequence.
ACCESSION   DAOFQD010000314 DAOFQD010000000
VERSION     DAOFQD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.322"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..776
                     /locus_tag="QBF46_004898"
     CDS             <1..776
                     /locus_tag="QBF46_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDQ6676968.1"
                     /translation="KYEDRSKYQTLENDKIILIQSPKNNGYASGNNIGIELALNQEDM
                     KYVWVLNNDTEVDKEALTHLISKCDSDKNIGICGSRLVYFTDREMQQGLGGVHNRWLC
                     TTKNYEMGRLVSKKYDDEVISNNIDYIIGASMFFSRECLETVGLMNEEYFLYYEELDI
                     CLRAKAKNFKLGICSDSLVYHKIGASTDGGKSMIADLCSIKNRLVITEKFYPQYYWTV
                     WLSLFVVAFNRARRGEFNKMKRCLNVMFNFKRNKGSKCH"
     gene            843..>1366
                     /locus_tag="QBF46_004899"
     CDS             843..>1366
                     /locus_tag="QBF46_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDQ6676969.1"
                     /translation="MKVAFLSAYDPLSTSSWSGTPYYMLKALSKRNISIEILGPVNSY
                     MVYMLKAYKLILRCFGKEYDYSRSKLLSKYYGRIFERKLKKIDGLDFIIAPAGSSQIA
                     FLETNIPIIYLSDTTYDQLKNYYPNLNKKTIINDEDASLIERKAIEKATVVSFPSKWA
                     MDFCKDYYRLDSDK"
BASE COUNT          501 a          155 c          271 g          439 t
ORIGIN      
        1 tgaagtatga ggatagaagt aaatatcaaa cattagaaaa cgataaaatc atattaatac
       61 aatctccgaa aaataatggg tacgcaagtg gtaataacat tggaatagag ctcgcactta
      121 atcaggagga tatgaaatac gtctgggttc tgaataatga tactgaagtg gataaagagg
      181 ctttaactca tttaattagt aaatgtgatt cagataaaaa tatagggatt tgcggttctc
      241 gtttagtcta ttttaccgat agagagatgc agcaaggact gggtggggta cataacagat
      301 ggttatgcac tacaaaaaat tatgagatgg gaagattagt ttccaaaaaa tatgatgatg
      361 aagtcattag caataatata gattatataa ttggcgcatc aatgtttttc tctagagaat
      421 gtttggaaac agttggattg atgaatgaag aatatttttt atactatgaa gagttagata
      481 tttgcctcag agcaaaagca aagaacttta aattaggtat ttgctcagat agtttggttt
      541 atcataagat aggtgcaagt actgatgggg gaaagagtat gatagctgac ctttgttcaa
      601 taaaaaatag gctggtcatt acagaaaagt tttatccaca atattattgg acggtatggt
      661 tgtcactctt tgttgtagca tttaaccgtg ctagaagagg tgagtttaat aagatgaaaa
      721 gatgtttgaa tgttatgttt aacttcaaac gaaataaagg tagcaaatgc cattagaata
      781 tgcatttaat gatggtgtta ttaaataaat gtatagtttg atatgttatt aaagggtatt
      841 taatgaaagt ggctttttta tctgcttatg atccactatc tacatccagt tggtcgggca
      901 caccttatta tatgctaaag gcattatcga agagaaatat ttccattgaa atattaggac
      961 cggtaaatag ctatatggta tacatgttaa aagcatataa attaatatta aggtgtttcg
     1021 gaaaggaata tgattatagt cgttcgaagt tgctttccaa gtattacggt agaatatttg
     1081 agagaaaatt aaaaaaaatt gatggtttgg attttattat tgcacctgca ggttcttcac
     1141 aaattgcttt tttagaaaca aatataccaa taatatatct atcggataca acatatgatc
     1201 aattaaaaaa ctattatccg aatttaaata aaaaaacaat tataaatgat gaggatgcaa
     1261 gtttaatcga acgcaaggct attgaaaaag caacagtcgt atctttccca tctaaatggg
     1321 caatggattt ttgcaaggac tattacagat tggattctga taaatt
//
LOCUS       DAOFQD010000315         1362 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.323, whole genome shotgun sequence.
ACCESSION   DAOFQD010000315 DAOFQD010000000
VERSION     DAOFQD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.323"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            70..1092
                     /locus_tag="QBF46_004900"
     CDS             70..1092
                     /locus_tag="QBF46_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDQ6676970.1"
                     /translation="MTITTVGIDLAKNVFAVHGVDQNGRTVLVKPKVSRAALPELIAS
                     LPPCVIGMEACSGAHYWARLFRQYGHEPRLMAAKFVSPYRMAGKSGKNDAADAQAICE
                     AVRRPHMRFVPVKDESQQAMQCLHRTRQGFIEEKTATYNRLRGLISEFGVIAPQSADA
                     LRHMVSVQKSSLPLQVQQCVDDLMEHVDRIEANITEYDRILSHIAKTDHRSQRLMELK
                     GVGPTTACALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDSYLRT
                     LLVQGARSVLIGAGKRTDSFSRWVCMLVERRGYWRAVVAIAAKNARLCWASLHYGDDF
                     RLYSVN"
BASE COUNT          334 a          312 c          356 g          360 t
ORIGIN      
        1 tcccctgatt atgcgcctga ttaactcttg tgcaatcatg gggtacacat cgtcccggag
       61 gaattcgtta tgactatcac gactgtcggt atcgatcttg ctaaaaatgt gtttgctgtt
      121 cacggtgttg atcaaaatgg tagaactgtt ctggttaagc ctaaagtatc gcgtgctgca
      181 cttcctgagt taattgcaag tttgcctccc tgtgttatcg ggatggaagc atgctccggg
      241 gctcactact gggcaagatt gtttcggcag tatggtcatg agccacgtct gatggctgct
      301 aagtttgtat cgccttatcg tatggccgga aaatcaggaa aaaatgatgc agctgatgct
      361 caggctatct gtgaagctgt tcgtcgtccg catatgcggt tcgtaccagt gaaagacgaa
      421 agccagcagg ccatgcagtg tttacatcgt acccgtcagg gttttatcga agagaaaaca
      481 gcaacgtata atcgtctgcg aggattgata tctgaattcg gtgtcatcgc gccacaaagt
      541 gctgatgccc tgcgccacat ggtttctgtg cagaagagct ctttaccgct tcaggttcag
      601 caatgtgttg atgatttgat ggaacacgtt gatcgcattg aagctaacat cactgaatat
      661 gaccgaattt tgtcccacat agccaaaaca gatcatcgca gtcagcggct gatggagcta
      721 aagggcgttg gtcccacaac ggcctgtgca ctggtcgcca gtatcggtaa tgcacatgat
      781 tttaaaaatg gacgtcaact ggcagcctgg ctggggctga cgccatcaca atacagcagc
      841 gggggaaagt cgaaactcgg caggatcaca aaagccggtg attcgtatct gcgaacgctt
      901 ctggttcagg gggctcgttc agtgcttatt ggcgccggga aaaggactga ctcattcagc
      961 cgttgggttt gtatgttggt tgagcgcaga gggtactggc gggctgttgt ggccatcgcc
     1021 gccaaaaatg cgcggctgtg ttgggcatca ctacattacg gtgatgattt ccggctgtac
     1081 tcagtcaact aaagctcgaa gctgtataac catctgacct gcaattcgtt gatgataaag
     1141 ggttagaccc cgtgaggcct atctggctat tgtacaggat atacgtatcc gcttaacgaa
     1201 caagaacctc acgcgcgtct ttcatcaggg cccgaatcga tgacgattca tcatggccgt
     1261 ttatagtacc gcagtctctc ccctttattt ttactgaaga gcagacagaa accataaata
     1321 ccggctttga catgccgggg aagtccttat agtacaataa tt
//
LOCUS       DAOFQD010000316         1359 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.324, whole genome shotgun sequence.
ACCESSION   DAOFQD010000316 DAOFQD010000000
VERSION     DAOFQD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.324"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..814
                     /locus_tag="QBF46_004901"
     CDS             <1..814
                     /locus_tag="QBF46_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDQ6676971.1"
                     /translation="VSLHGVMEAGVAQPVYTPYAAQLDKVLVQEGQQVKAGQKLFELV
                     APVPADEQDKALALSQAWQTSARGAMALDKNGAAKQVVADQMARQYAIQQRASAVELQ
                     RLQLVALEDGVVHDVEQTLRPGSWVSPGTRIASVIDTHRWRVKALVSETDLARLRDGA
                     TARVYQQGRWQPLAGAVVTIDHDAIRRLPNLMLAQSHGGPVALNPVAPANELRPAAVW
                     YRVVIEGISAAPVSKEQLVRVDVASDRQSLAHSWVNSVMLMLIQQTGFGKEG"
     gene            1193..1359
                     /locus_tag="QBF46_004902"
                     /pseudo
     CDS             1193..1359
                     /locus_tag="QBF46_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011846847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          254 a          400 c          444 g          261 t
ORIGIN      
        1 ggtctccctg cacggcgtga tggaggcggg cgtggcgcag ccggtttaca cgccctatgc
       61 cgcccagctt gacaaggtgc tggtgcagga aggccagcag gtcaaggcgg gccagaagct
      121 gtttgaactg gtggcccccg tcccggcgga cgagcaggat aaggcgctgg cgctaagcca
      181 ggcgtggcag accagcgccc gcggcgcgat ggcgctcgat aaaaacggcg cggcgaagca
      241 ggtggtcgcc gaccagatgg cgcgccagta cgccattcaa cagcgggcca gcgccgttga
      301 gcttcagcgg ctccagctgg tggcgctgga ggatggcgtg gtgcatgacg ttgagcagac
      361 cctgcgcccc ggcagttggg tctcgcctgg cacccgcatc gcaagcgtga tcgacaccca
      421 ccgctggcgc gtgaaggcgc tggtcagcga aaccgacctg gcccgcctgc gcgacggcgc
      481 gacggcgcgg gtttatcagc agggccgctg gcagccgctc gcaggcgcgg tagtgaccat
      541 cgaccacgac gccatccgcc gtctgcctaa tctgatgctg gcgcagagtc acggcggccc
      601 ggtggcgctg aacccggttg cgcccgccaa cgaactccgt cccgccgccg tctggtaccg
      661 ggtagtgatc gaaggcatct ccgccgcgcc ggtcagcaaa gagcaactgg tacgtgttga
      721 cgtggcgagt gaccgccaga gcctggctca cagctgggtc aacagcgtta tgctgatgct
      781 tatccagcaa actggctttg gtaaagaggg ttagcgtgcg attttcgcgc caggccgcct
      841 ggcctggcgc aggttttctc cccaccgcgg ttttaggtta aaccccgttt cgccagagca
      901 aactggcacc tacgttttgt tagtctgttc ggtgttctgc aactggaccc ggaggttata
      961 ttacttgcag gcgtgcagct gatgctcgca agactcgttg gtttctgttt ccggaggtgt
     1021 ggttatgcaa aaaattgtct gcaaaacgcc acgattgtca tcactggcat gtcgagttgt
     1081 ttcggccacg aggccgccct gcggctggcg gagataggtg ccaacgccat gctggcagcc
     1141 agacggaact ggaggcgctc gccgccgata ttgtcacggc gggcggcaat gcgaaagtga
     1201 tctgaaaatt accgaacatt acaccgatac agcgggcttc actgagcatg ttttcaccct
     1261 gatgcatctg ctgggattcg ctttttcgcc aaggatccgg gatattcatg agaagcggct
     1321 ggttattcat caaaaggccg agcgttattc cgggaaaca
//
LOCUS       DAOFQD010000317         1332 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.325, whole genome shotgun sequence.
ACCESSION   DAOFQD010000317 DAOFQD010000000
VERSION     DAOFQD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.325"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            54..770
                     /gene="nanC"
                     /locus_tag="QBF46_004903"
     CDS             54..770
                     /gene="nanC"
                     /locus_tag="QBF46_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDQ6676972.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            790..>1332
                     /gene="nanM"
                     /locus_tag="QBF46_004904"
     CDS             790..>1332
                     /gene="nanM"
                     /locus_tag="QBF46_004904"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDQ6676973.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAE"
BASE COUNT          416 a          268 c          304 g          344 t
ORIGIN      
        1 ttgtttatgc gggctttgta ttgctttctg tatcctacaa atgagtgaaa tttatgaaaa
       61 aggctaaaat actttctggc gtattattac tgtgcttttc gtcaccatta atttctcagg
      121 ctgcgacact ggacgtacgt ggtggatatc gtagtggaag ccacgcctat gagactcgac
      181 tcaaagtcag tgagggatgg caaaatggat ggtgggcaag catggaaagt aatacctgga
      241 ataccattca tgataataaa aaggaaaatg ccgcactcaa tgatgttcag gttgaagtta
      301 attacgcgat taaacttgat gatcaatgga cggtgcgccc gggaatgtta acgcatttta
      361 gcagcaacgg cacacgctac ggaccctacg taaaactgtc ctgggacgcg acaaaagatc
      421 ttaattttgg cattcgctat cgttacgact ggaaagctta ccgacaacaa gacttatccg
      481 gtgatatgtc tcgtgataac gttcatcgtt gggatggata tgtcacttac catattaata
      541 gtgatttcac cttcgcatgg caaacgacgc tatacagcaa acagaacgat tatcgctatg
      601 caaaccataa gaaatgggcg acggaaaatg catttgttct acaataccat atgacgcccg
      661 atattacgcc atacatagaa tatgactacc tggaccgtca gggtgtttac aacggcagag
      721 ataatttatc ggaaaacagt tatcgcattg gtgtgtcatt taaactgtag tagacaggag
      781 acagtcacaa tgaataaaac aataacggcg cttgctatcc taatggcttc atttgccgca
      841 aacgcgtctg tattaccgga aactcctgta ccatttaaaa gtggtaccgg agcaattgat
      901 aacgacactg tctacattgg tttaggtagc gcaggtacgg catggtacaa gctggataca
      961 caggccaaag ataaaaaatg gacagcgtta gctgcattcc ctggtggacc aagagatcaa
     1021 gcaacctcgg catttattga tggcaatctg tatgtgtttg gcggcattgg caaaaacagc
     1081 gagggcttga ctcaggtatt taatgacgta cacaaataca accccaaaac caatagctgg
     1141 gttaaattga tatcgcacgc accgatgggc atggcgggcc atgtgacttt tgtacacaac
     1201 ggcaaggctt atgttactgg cggtgttaac cagaatatct tcaatggcta ttttgaagat
     1261 ctcaacgaag ctggaaaaga ttcaaccgct atagataaaa tcaatgcaca ctattttgac
     1321 aaaaaagcag aa
//
LOCUS       DAOFQD010000318         1318 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.326, whole genome shotgun sequence.
ACCESSION   DAOFQD010000318 DAOFQD010000000
VERSION     DAOFQD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.326"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            470..766
                     /locus_tag="QBF46_004905"
     CDS             470..766
                     /locus_tag="QBF46_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDQ6676974.1"
                     /translation="MIMFHIAAINNAIIGRDTLIASKVFISDHNHGIFSKSDIHSSPT
                     IIPSSRSLESAPVYIEERVWIGENVTILPGACIGNGVVIGANSVVRGEILNNVI"
BASE COUNT          420 a          173 c          255 g          470 t
ORIGIN      
        1 ccgcagtata ttttagcata tagtttctat atgtgtttct tcatccccct ttttatacgt
       61 ggtgctggct atataaatcc ctatgtgttt atgttatttt catcaatatt tttatgcaaa
      121 tatcatgcta aaattatctt gatgaaatct aatgtcaaaa tggctatata atagtagatt
      181 atgttatcat taatacgtaa ataacatata ttctaaccag ggcataaata atgtgcataa
      241 aaaaattaag ttaattaaac gatatggcct ttatggtggt cttaggcttc ttaaagatta
      301 ttcttaacaa aagttttatt ttgttcaaat gttagggtta ttagattttc atgttatctt
      361 agaaaagatg gaagtgttag ttttggaaaa gggtttacat caggtgtagt attaggaggt
      421 gatacattta tggatgctat agtgtctatt ggagaaaatg ttcaaattaa tgattatgtt
      481 ccacatcgcg gctattaata atgccattat tggtagagat acattaatag caagtaaagt
      541 atttattagt gatcataatc atggtatttt ttctaaatct gatatccata gttcaccaac
      601 tattattcct tcatctaggt ctcttgaatc tgcacctgtg tatattgaag agcgtgtgtg
      661 gattggtgaa aacgtgacaa tattaccagg tgcgtgtata ggtaatggtg tagttattgg
      721 cgcaaatagt gttgttcgtg gtgagattct taataatgtg atctgagttg acccccgaaa
      781 acaccagaca gtagatgtat cttaaaataa gaggtaggct tggacatata tctaacttaa
      841 gatacaatcc ctgtctggaa ataagagggc aagaacagaa aatgtttcga tttgggaaag
      901 ataggatatg gcttcttatg tagtatataa tatcctgcat tcattcggat aacttcctat
      961 ggaagtgtac tttgctctgt ctgttttcat ttgttgaaaa tttatgttaa taagaagctt
     1021 tagataacca cttaggaact gtatgtttga tttgtccaaa agttatattg ttgtaagtgc
     1081 gacggcgctg gcctccggag gtgcattaac tatattaaag caatttataa aacatgcatc
     1141 acaaaattca aatgactata ttatgtttgt atctgcggga ttagagttgc cggtctgtga
     1201 taacatcatt tacatagaaa acacaccaaa aggatggttg aaaagaatat attgggattg
     1261 gttcggttgt cgaaagttta tctctgaaca taagattaac gttaagaaag taatttct
//
LOCUS       DAOFQD010000319         1296 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.327, whole genome shotgun sequence.
ACCESSION   DAOFQD010000319 DAOFQD010000000
VERSION     DAOFQD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.327"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>1296
                     /locus_tag="QBF46_004906"
     CDS             <1..>1296
                     /locus_tag="QBF46_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDQ6676975.1"
                     /translation="DNITKLKSLFPEAFNENGIDFDVLKQLLGENVDEKEERYGLNWH
                     GKRQARQLALTPSRGTLRPCKDESVDWHNTKNLMIEGDNLEVLKLLQKSYAGKVKLIY
                     IDPPYNTGKDFVYPDNFQDNMKNYLEITGQTEDGARLSTNTETSGRYHTDWLNMIYPR
                     LKLARNLLKEDGVIFISIDDTEVDNLKKVCSEIFGEENFVANIVWQKKYSPQNDATYF
                     SDMHDHILVYAKQRKTSKNDSNGWNIDFLPRSDEQNAAYKNPDNDPRGVWKSVDLSVK
                     TYSKANDYSITTPSGRIVTPPASRCWQVSEKRFVELCKENKIWFGENGNNVPSIKRFL
                     TEVQDGVVPTTWWSYKECGHNQEAKQELKKLMEGESVFFDTPKPLRLLDRILHLATTN
                     DKDCIILDFFAGSGTTAHATLNKNIADSGSRRYIAVQLPE"
BASE COUNT          450 a          229 c          254 g          363 t
ORIGIN      
        1 gataacatca ccaagcttaa atcattgttt cctgaagcct ttaatgaaaa cggcattgat
       61 tttgacgttt taaaacagct tttgggtgaa aatgtagacg aaaaagaaga acgctatggc
      121 ctgaactggc atggtaagcg tcaggctcgt cagcttgctc ttaccccttc ccgtggcacc
      181 ttacgcccat gtaaagatga aagtgttgat tggcataaca ccaaaaacct catgatcgaa
      241 ggtgataacc ttgaagtgtt aaagcttctt caaaaaagtt atgccggaaa agtgaagctt
      301 atttacatcg atccgcctta caataccggt aaagattttg tctatcctga caacttccaa
      361 gataatatga aaaattatct tgaaattact ggacagacag aagacggagc acgtttaagc
      421 acaaatacag aaaccagtgg gcgttaccat actgattggt taaatatgat ttacccacgt
      481 ttaaaacttg caagaaactt gttgaaagaa gatggggtta ttttcatttc cattgatgat
      541 actgaagttg ataatttaaa aaaagtttgc tcggaaattt ttggtgaaga gaactttgtt
      601 gctaatattg tatggcaaaa aaagtactca ccacaaaatg atgcaacata ttttagtgat
      661 atgcatgatc atattttagt ctatgcaaag caaaggaaga cttcaaaaaa tgattcaaac
      721 ggttggaaca tagattttct accaagatca gacgaacaaa atgcagcata taaaaacccc
      781 gacaatgacc ctcgtggagt gtggaagtct gtagatttat cagtcaaaac ttattccaag
      841 gctaatgatt actcaattac cacaccatca ggacgtattg taactcctcc cgccagtaga
      901 tgctggcagg tttctgaaaa acgttttgta gaattgtgta aagaaaataa aatttggttt
      961 ggagaaaatg gtaataatgt tccatcaatc aaaagattcc tgactgaggt ccaagatggt
     1021 gttgttccta caacatggtg gtcatataaa gaatgtggac ataatcaaga ggccaagcag
     1081 gaactaaaaa aactcatgga gggagaatct gtattctttg acacacctaa gccattgaga
     1141 ttattagata gaatattaca cttagcaaca accaatgata aagattgtat tatcctagat
     1201 tttttcgcag ggtcaggtac cacggctcac gcaactttga ataaaaatat tgctgacagt
     1261 ggttcacgcc gatatattgc tgtgcaatta cctgaa
//
LOCUS       DAOFQD010000320         1289 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.328, whole genome shotgun sequence.
ACCESSION   DAOFQD010000320 DAOFQD010000000
VERSION     DAOFQD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.328"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            71..>1289
                     /locus_tag="QBF46_004907"
     CDS             join(71..395,395..>1289)
                     /locus_tag="QBF46_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HDQ6676976.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGIAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRAKKLDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCL"
BASE COUNT          328 a          307 c          378 g          276 t
ORIGIN      
        1 cagacaacca atatcactta aataagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt atagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtcgccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac
      481 gtcattctca gacgaaccga tgactggatg gatggccgcc gcagtcgtca cactgatgat
      541 acggatgtgc ttctccgtat acaccatgtt atcggagagc tgcccacgta tggttatcgt
      601 cgggtatggg cgctgcttcg cagacaggca gaacttgatg gtatgcctgc gatcaatgcc
      661 aaacgtgttt accggatcat gcgccagaat gcgctgttgc ttgagcgaaa acctgctgta
      721 ccgccatcga aacgggcaca tacaggcaga gtggccgtga aagaaagcaa tcagcgatgg
      781 tgctctgacg ggttcgagtt ctgctgtgat aacggagaga gactgcgtgt cacgttcgcg
      841 ctggactgct gtgatcgtga ggcactgcac tgggcggtca ctaccggcgg cttcaacagt
      901 gaaacagtac aggacgtcat gctgggagcg gtggaacgcc gcttcggcaa cgatcttccg
      961 tcgtctccag tggagtggct gacggataat ggttcatgct accgggctaa tgaaacacgc
     1021 cagttcgccc ggatgttggg acttgaaccg aagaacacgg cggtgcggag tccggagagt
     1081 aacggaatag cagagagctt cgtgaaaacg ataaagcgtg actacatcag tatcatgccc
     1141 aaaccagacg ggttaacggc agcaaagaac cttgcagagg cgttcgagca ttataacgaa
     1201 tggcatccgc atagtgcgct gggttatcgc tcgccacggg aatatctgcg gcagcgggct
     1261 tgtaatgggt taagtgataa cagatgtct
//
LOCUS       DAOFQD010000321         1283 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.329, whole genome shotgun sequence.
ACCESSION   DAOFQD010000321 DAOFQD010000000
VERSION     DAOFQD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.329"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            78..772
                     /locus_tag="QBF46_004908"
     CDS             join(78..324,324..772)
                     /locus_tag="QBF46_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDQ6676977.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDVQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(856..>1283)
                     /locus_tag="QBF46_004909"
     CDS             complement(856..>1283)
                     /locus_tag="QBF46_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ6676978.1"
                     /translation="YLAVVIDLFARNVVGWSMKPTLSRELALDALLMAVWRRKPEENV
                     IVHSDQGSQYGSDDWQRFCRANNLVPSMSRRGNCWDNAVAESFFSSLKKERIRKRIYK
                     TRDMARADIFDYIEVFYNRARRHSHLGGVSPEAFEKASQ"
BASE COUNT          311 a          347 c          315 g          310 t
ORIGIN      
        1 atgttttata atgctggatg ggtgatgcct ctaattagtt gaatctgatg tataatacgg
       61 gcttttgagg ttatctcatg accagcgtta atattcattg tccccgttgt cagtcagctc
      121 aggtttaccg ccatggtcag aaccctaaag gccgtgacag acttcgctgc cgtgactgcc
      181 accgtgtgtt tcagttcact tatacttatc aagcacgtaa gccgggtatg aaagagctga
      241 taactgaaat ggcctttaat ggtgccgggg ttcgcgatac cgccaggaca ctgaaaattg
      301 gtagtaacac cgtcatccgg actttaaaaa actcgcgcca aagcgaataa cttcttcgcc
      361 tgttgcccat gctgatgtgg cgtttatctg cgagcttgat gtgcaatgga gctacgttgg
      421 cagtaaagcc cggcaacact ggctcgggta cgcgtacaac accaaaacag gcggtgtact
      481 ggcctacact tttggccccc gaaccgatca aacgtgccgg gagctactgg cactgcttac
      541 acccttcaac atcggcatgc tgaccagcga tgactggggc agctatggcc gggaggtgcc
      601 gaagaataag catctgaccg gcaaaatatt cacccaacgc attgagcgta ataatctgac
      661 gctacgcacc cgcattaagc ggttgggtcg taaaacaatc tgcttctcac gctcagttga
      721 gatccacgaa aaagtgatcg gagcatttat cgaaaaacac atgttctact aattggatgc
      781 actacccagt atgtaactgg gtttgtccat aaaatgcacc tggagtgacc acgctccggt
      841 agacagtttc tgtcctcact gcgaggcctt ttcgaaggcc tccgggctga cgccgccgag
      901 atgactgtgg cgccgggccc ggttgtagaa cacttcaata taatcgaaaa tatccgcccg
      961 ggccatgtcc cgggttttgt atatccgctt cctgatgcgc tcttttttga gtgaactgaa
     1021 gaacgattct gccactgcat tatcccagca gttgccgcga cggctcatgc tcggtaccag
     1081 attattggcc cggcagaacc gctgccagtc atcactgcca tactgactgc cctgatcgct
     1141 gtgcacaatg acgttttctt caggtttacg tcgccagacc gccatcagca gcgcatccag
     1201 tgccagctcg cgcgacagtg tcggcttcat cgaccagccc accacattac gggcaaaaag
     1261 atcgataacc actgccaggt aca
//
LOCUS       DAOFQD010000322         1283 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.330, whole genome shotgun sequence.
ACCESSION   DAOFQD010000322 DAOFQD010000000
VERSION     DAOFQD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.330"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            112..1283
                     /locus_tag="QBF46_004910"
                     /pseudo
     CDS             112..1283
                     /locus_tag="QBF46_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240260.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          366 a          238 c          327 g          352 t
ORIGIN      
        1 cgtcggcatg gttctagcga tcagatgtca ccgacagaat atgaaaacca gtattatcaa
       61 cggctcggaa gtgtctagat tatctgtggc gattcatatc ttggaatata aatgttaaaa
      121 aaattaataa ctttttctgc catcctgtgt acgggacaag catgggctgc atgtgtggat
      181 ggtgcagctg gcccaaacgg cgtagatagt gccaccgaag gtagtacctg cgataacagc
      241 ggtacgtcat atatagggaa taataccgct tatgcgagcg ggtcaggttc tgttcttaat
      301 ttcttacctg gaactgtaat cagtagtaat ggtgttgctg ccgtttatac gcttgccgtg
      361 ggaggagtca atagtggcgg ttcgatccct ggagctggag caacggttca tgcaaaagga
      421 gatttaacag ttaatgggaa tgctctgggc agtactcgga gtatttttat cttttctggt
      481 acagatgctg ggggaaaacg taactcactt cagatagatg gaaacctgac tgcaacgagg
      541 actggcggtc tttatggggc tgttatcgaa aatacaggcg gggacttatt ggttgcaggt
      601 aaaacctctc tgacatccaa tgtctctgat gcacttcgta atagagggga tgctaatagt
      661 acattcaggg ggtccacatc gataaatgta agcggatcca atggaatatt tcaatctgga
      721 gggaccgtta attttaatgg tgatttaaca atcaacacca caactggtaa cggaataaaa
      781 acaagtgggg gctattaaat gcccgtgagc tttatattga tgtgaaaagc ggagttggaa
      841 atggtattgc gatatctgac cgcggaatag tcaatatttc tgggggggaa atcatcaccg
      901 aagggaaagg ttccactgct atttccctca atggaggggc tttttcttct acagatgcat
      961 tgtcaatcct tactaaatct aatgactcgc aaggaatagt tctttctggc gatgcaaaag
     1021 cgatattagc tcgtactgat attcaaaccc aaggtgataa tgctaatggt ataaatatca
     1081 taaatactcc aaccaatggt gtaacattgt acttccagga cgctatatcg atagaaaccc
     1141 tggggggcgg gagtaatgca attgcatcca agaataatcc ggcaaatgga tttgttcttc
     1201 ccgcgggaag catgctcgtc acccagggtg aaaattcgca tggtattgat atgcaaattg
     1261 agtctggtgg tggtgactta aca
//
LOCUS       DAOFQD010000323         1265 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.331, whole genome shotgun sequence.
ACCESSION   DAOFQD010000323 DAOFQD010000000
VERSION     DAOFQD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.331"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            94..738
                     /locus_tag="QBF46_004911"
     CDS             94..738
                     /locus_tag="QBF46_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676979.1"
                     /translation="MNKRLNMLYVNNINALNNYREKYSDNNLHGPLLLKLKNYFHQHN
                     KLMVIGQETYGWCNSPDINEQLETYEEFDFGVSYYSSPFWNIIRKVERALGIEPYAIA
                     WSNLNRFDVDCGSPDYTELARDISSFDYILKEEINILTPDICVFFTNHKYDYRLTSLY
                     EDLMFENINGLPEKHFVRLYHPDLPEHTIRAPHPKTIRIKGWENDFIKYIEAIK"
BASE COUNT          428 a          197 c          222 g          418 t
ORIGIN      
        1 ccctattata aaaagaggtt gactgtctac aaatcgaata atatcataat aactatttat
       61 gttattatga tattagttaa aaatggaaag aatatgaata aaagacttaa tatgctatat
      121 gtcaacaaca ttaacgcatt aaataattac cgtgagaaat attcagataa taacttacat
      181 ggccccctcc ttttaaagct aaaaaattat tttcatcagc ataataaatt gatggttata
      241 ggacaggaaa cctatggatg gtgcaactct cccgatatta atgaacagct tgaaacatat
      301 gaggagttcg attttggcgt atcatattat agcagtccat tctggaatat cataaggaaa
      361 gtagagcgag cgcttggtat tgaaccatat gcaattgcat ggtcaaatct gaatcggttt
      421 gatgttgatt gcggatcacc agattatacc gagctagcta gagatatatc atcgtttgat
      481 tatatattaa aggaggaaat taatattctt actcctgata tatgtgtttt cttcacaaat
      541 cataaatatg attatcgact aacgtcttta tatgaagatt taatgtttga aaacataaat
      601 ggattgccag aaaaacattt cgtaagatta taccatccag acctcccgga acatacgatt
      661 agagcgcctc accctaaaac aatcagaata aaaggatggg aaaatgattt cataaaatat
      721 atagaggcta ttaaatgatt atgcagatgc gatcttatta atatgaataa acaaaattgt
      781 gttactaatt gcataacagc aatataagtt attctatagt ttatctaggc cacggcgtgt
      841 aggtggcctt aaacgaggag tatgattttt atcatttatt gttgaaacag tcggtataaa
      901 atccatgtta ttatctgcat tgactcgttc gtgcttagga cggcttggga aacgtcgaat
      961 ctcatcaggt gttggtttgt aagtatcact gttaaactta gcttgtttct catattcagc
     1021 acgagaaaca tgatgaccta atacttcatc aaagacaaag ttttcagtaa tacgtttatc
     1081 atgttctgta ctgacaggat catgcttaac tctgcttgat ctagcccata caaatttttc
     1141 tttgaagaaa tctatgacaa gagttgtgaa acgtttgaaa atattatcct gacgtggagt
     1201 agtcttatcc tttctttcat ggtcaggggc gcggttttaa tttaattatc tattttcctt
     1261 gtcat
//
LOCUS       DAOFQD010000324         1235 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.332, whole genome shotgun sequence.
ACCESSION   DAOFQD010000324 DAOFQD010000000
VERSION     DAOFQD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.332"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(65..238)
                     /locus_tag="QBF46_004912"
     CDS             complement(65..238)
                     /locus_tag="QBF46_004912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676980.1"
                     /translation="MMHINKIVGWLFESMPECLFKSLSEPVFSEIDGIVQTNMLKASW
                     MQDAMRSTAEKDE"
BASE COUNT          341 a          256 c          269 g          369 t
ORIGIN      
        1 tgaccccata acaggaatat ttacgttcca ctgccaatca aaccaattag agatttactt
       61 tctgctactc atctttttcg gcagtactgc gcatggcatc ttgcatccaa gatgccttca
      121 acatgtttgt ttgtacaatg ccatcaattt ccgagaatac aggttcagat aatgacttaa
      181 acaggcactc aggcatactt tcaaaaagcc agccaactat cttgttgata tgcatcatcg
      241 cccccttagt cgcttaccaa aattatagtt gaatatattc gattttacag atatgttgtt
      301 aactatttct gggctgcgat tactgtaatc agatgtccac atacggagta agaatgtgca
      361 gttctccacc tttccgcttt ccaaaaagct gagggcagag gcatgaatac gatacagttg
      421 ctgacatctt cggaatgcta aagtggcgag ttaccttact attaatagat aaattgggat
      481 gctggcgttt cactgttagg ttatacagta tttacgctca tatgtcggtc tgagtgatat
      541 ctggtggcag tatggattgt ctttaactcg ttaaaaaagg catggtatac atatgattat
      601 tcagatttgt ttggtaatga gaggaacatc tttttctttg taccgagaat tcctatcgtt
      661 ccattctagt ctggctgaag cgacatttaa cgcgctatgg ccagcataag tgcaggcccc
      721 caaccgaaag aatagggggg gctttgtcct gcgacagcag agcaataaca tttagcgatg
      781 tattagcagc aaaatgacca ctggttcttg aactgctaac ctatgaccgt ctgaaaatga
      841 agggtaaagc gttgcaatga cgaacacaat gtacagttct ggtaactcgg cacaaatcat
      901 ctttgcggag gtattaatga acctgcacag tgacggcatt tttcaagcat tcagtgccgg
      961 ttctaatccg gccagttcgc ctcaccccat gacactcagc atactaaaag gtcagggggg
     1021 caatactgat actttacaga gtgtaacata tgcaggattt tcagcattcg agtggtccgc
     1081 gtattgattt tatctttaca tattgtagaa aaatgtcagg cgaggattgt ctcgtgtttc
     1141 ctggtggagc aataaggctt tgttgaataa atcagatttc gggtaagtct cccccgtagc
     1201 gggttgtgtt ttcaggcaat acgcacgctt tcagg
//
LOCUS       DAOFQD010000325         1225 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.333, whole genome shotgun sequence.
ACCESSION   DAOFQD010000325 DAOFQD010000000
VERSION     DAOFQD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.333"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..1029
                     /locus_tag="QBF46_004913"
     CDS             <1..1029
                     /locus_tag="QBF46_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6676981.1"
                     /translation="DEAVRAVADAVRLSRAGLREGSKPVATFLFLGPTGVGKTELAKA
                     LAESIYGDEHALLRIDMSEYGERHTVARLVGAPPGYVGYDEGGQLTEKVRRKPYSVLL
                     LDEIEKAHPDVYNILLQVFDDGRLTDGKGRVVDFTNTIIIATSNLGSDIIQRRLKARG
                     AADEEYEKTKAEVMDVLRGHFRPEFLNRIDEIIVFHALGKEEIRHIVGLQLDRVARSA
                     ASQGVTLTFDQTLIDHFAEEGYKPEFGARELKRLIRSELETALAREMLGGGIGKGDHA
                     SARWDDKAERVVFERKEPLQTPAEPEQPDAAKATEPEQPDAAKATETPHGDAGKGSRK
                     KKSASDAS"
     gene            1029..1220
                     /locus_tag="QBF46_004914"
     CDS             1029..1220
                     /locus_tag="QBF46_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676982.1"
                     /translation="MAGEAAVSDPKGLAWAASLASIAVAFVAAGHAVVYKRDPRSTTP
                     WGLLISGGTALPLAGHPFA"
BASE COUNT          225 a          395 c          424 g          181 t
ORIGIN      
        1 gacgaggcgg tccgtgccgt ggccgatgcc gtgcggctgt cccgcgccgg cctgcgcgaa
       61 ggcagcaaac cggtggctac cttcctgttt ctgggcccga ccggggtggg caagaccgag
      121 ctcgccaagg cattggccga atcgatctac ggcgatgagc acgccctgtt gcgcatcgac
      181 atgtcggaat atggcgaacg ccataccgtg gcgcggctgg tgggcgcgcc tccgggctat
      241 gtcggttacg acgaaggcgg tcagctcacc gagaaggtgc ggcgcaagcc ctacagcgtg
      301 ctgctgctcg acgagatcga gaaggcacac cctgacgtct acaacatcct gctgcaagtg
      361 ttcgacgacg gtcgcctcac cgacggcaag ggccgggtgg tggatttcac caacaccatc
      421 atcatcgcca cgtccaacct gggttcggac atcatccagc gacggctgaa ggcgcgtggg
      481 gcggccgacg aggagtacga aaagaccaag gccgaggtca tggacgtgct gcgcggccac
      541 ttccggcccg agttcctcaa ccgcatcgac gagatcatcg tcttccatgc gctgggtaag
      601 gaggagatcc gccacatcgt cggcctgcag ctcgatcgcg tggcgcgcag cgccgccagc
      661 cagggcgtga cgctcacttt cgatcagacg ctgatcgacc atttcgcgga agaaggctac
      721 aaacccgagt tcggtgcgcg tgaactcaag cgcctgatcc gcagcgagct ggaaactgcg
      781 ctggcgcgcg agatgctcgg tggcggcatc ggcaagggcg atcacgccag cgcacgctgg
      841 gacgacaagg ccgaacgcgt ggtgttcgaa cgcaaagagc cactgcagac cccggccgag
      901 ccggagcagc cggatgccgc gaaggcgacc gagccggagc agccggatgc cgcgaaggcg
      961 accgagacgc cgcacggcga cgctggcaaa ggctcgcgca agaagaagtc ggcgagcgac
     1021 gcatcttgat ggcgggcgag gctgccgtgt ccgaccccaa gggcctggca tgggcagcct
     1081 ccctggcctc gatcgcggtg gcgttcgtgg ccgcgggtca cgcggtcgtc tacaagcgtg
     1141 atccgcggtc gaccacgccg tgggggctgc tcatcagcgg cggcactgcc ctcccgctcg
     1201 ctggccatcc gtttgcgtga cggtg
//
LOCUS       DAOFQD010000326         1184 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.334, whole genome shotgun sequence.
ACCESSION   DAOFQD010000326 DAOFQD010000000
VERSION     DAOFQD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.334"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..468
                     /gene="hsp20"
                     /locus_tag="QBF46_004915"
     CDS             <1..468
                     /gene="hsp20"
                     /locus_tag="QBF46_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003847761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock protein sHSP20"
                     /protein_id="HDQ6676983.1"
                     /translation="PAASGPVSPILQLHREIDRLFDDAFRGFGFPALNMPQWPSDWSG
                     MLKPALDIQETDKQYKIALEVPGVEEKDIQITLDNDVLMVRGEKRQEQEKKEGGFHRV
                     ERSYGSFQRALNLPDDANQDSIKASFKNGVLTVTIDKREVSAPKQGRSIPING"
     gene            574..>1184
                     /locus_tag="QBF46_004916"
     CDS             574..>1184
                     /locus_tag="QBF46_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpC"
                     /protein_id="HDQ6676984.1"
                     /translation="MARKQCQVCGQPATVRVEANLNGRHSTMLLCDDHYRQLVRQQKR
                     TVSPLEALFGSRSGLFEDFLGSDFFRIGDDATPVAADTDDVVDASFGEPAAAGSGAPR
                     RRGSGLASRISEQSEALLQEAAKHAAEFGRSEVDTEHLLLALADSDVVKTILGQFKIK
                     VDDLKRQIESEAKRGDKPFEGEIGVSPRVKDALSRAFVASNELG"
BASE COUNT          241 a          367 c          375 g          201 t
ORIGIN      
        1 ccagcggcga gcgggccagt cagcccgatc ctgcaactgc atcgggaaat cgaccggctg
       61 ttcgatgacg cattccgggg cttcggtttt ccggcgttga acatgccgca gtggccatcc
      121 gattggtcgg gcatgctgaa gccggccctg gacatccagg aaaccgacaa gcagtacaag
      181 attgccctgg aagtgcccgg tgtcgaggag aaggacatcc agatcaccct cgacaacgac
      241 gtgctgatgg tgcgtggcga gaagcgccag gaacaggaga agaaggaagg tggcttccac
      301 cgtgtggagc gctcctacgg cagctttcag cgtgccttga acctgcctga cgacgccaac
      361 caggattcga tcaaggcatc gttcaagaac ggggtgctca cggtcacgat cgacaagcgc
      421 gaggtcagcg cgccgaagca gggacgctcg atcccgatca acggctgacg tcgccggctc
      481 gttcgtcaca agaaaaaccc gtcccgagac gaggtgtcgc ggctcgcgat gcctcgtcgc
      541 ctcaaccttt tcaaccttct aaggagcatc agcatggcca gaaaacaatg ccaggtctgc
      601 ggccaacccg ccaccgtgcg ggtggaagcc aatctcaacg gtcgtcacag caccatgctg
      661 ttgtgcgacg accattaccg gcaattagtg cgccagcaaa agcgcaccgt ttcgccgctg
      721 gaagccttgt tcggttcgcg cagcggcctg ttcgaggact tcctcggcag tgacttcttc
      781 cgcatcggcg acgacgcgac gccagttgcc gccgataccg atgacgtggt cgatgcctcg
      841 tttggcgagc ccgccgccgc aggttcgggt gcgccgcgcc gtcgcggcag tgggctggcc
      901 agccgcatca gcgaacagtc ggaagccttg ttgcaggagg ccgccaaaca cgctgccgaa
      961 tttggccgct ccgaggtgga taccgaacat ctgctgctgg cgctggccga cagcgacgtg
     1021 gtcaagacca tcctgggtca gttcaagatc aaggtcgatg acctcaagcg gcagatcgag
     1081 tctgaggcca agcgcgggga caagcccttc gagggcgaga tcggcgtgtc gccgcgcgtg
     1141 aaggatgcgc tcagccgcgc cttcgtggcc tccaatgaac tcgg
//
LOCUS       DAOFQD010000327         1183 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.335, whole genome shotgun sequence.
ACCESSION   DAOFQD010000327 DAOFQD010000000
VERSION     DAOFQD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.335"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..924)
                     /locus_tag="QBF46_004917"
     CDS             complement(<1..924)
                     /locus_tag="QBF46_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDQ6676985.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYSRQHETGGDAIYCGLYGHHIKNGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHEKIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     QLNQLVESNEKELKDCNLPGKRDKRRAKILAS"
     gene            complement(1009..>1183)
                     /locus_tag="QBF46_004918"
     CDS             complement(1009..>1183)
                     /locus_tag="QBF46_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDQ6676986.1"
                     /translation="NEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFI
                     KFKLNKYKRKLFG"
BASE COUNT          370 a          214 c          168 g          431 t
ORIGIN      
        1 tgatgctaat atttttgctc gtctcttatc tctcttgcca ggaagattac aatcttttaa
       61 ttctttttca ttgctttcaa ctaattgatt caattgttta aaatcaccat taagtagaac
      121 attccacaaa taacgagttt tatgataagc tctattttga ctcaataatg tatgaatttc
      181 ttctttccgg ttacctttgt ataactgcat cacagaagag tagatttttt cgtgcgcaaa
      241 tgactcatgt cgataatgag tgatggttct acatgagttc attaaggaat tttctctata
      301 gttatgctta tacatataat atggaaccgc atacatatcg catgtaatta gcagtcgata
      361 gataaaatcc agatcttctc ctagtcgtaa atcttcatca aaaaaaagat tattctcttc
      421 cagaagttgt tttcttataa acaagcaccc tatatggaat ctaacctttt tataaaaaaa
      481 gtcgaaaagt aaagatcctt cattaaactc tgtatttata ggtgagtaag taacaccatt
      541 ttttatatga tggccataaa gcccacaata aatggcatct cctcccgtct catgttgtct
      601 ggaatacatc ttcagcagat agttgggatc tatctcatca tcatcatcca taaaacatat
      661 aaactgtcct tgcgccttgc ggatacctgt attcctggca gacgaaacac cgccattttc
      721 tttattgata ataattaatc gagagtgggt taacccatac tctctcaaaa cgtttgatgt
      781 gttgtcttta gaaccatcat taacaataac aatttcaaaa tttgggtaag tttgtttttc
      841 taatttcgtt agagtttcag taatgtaatc agatgaatta tacgtcggta ttattatgga
      901 tactaaaggg taatcattgc tcatcgtgtt ctcttgcctg aactaataaa atatgatagt
      961 actcatcctt aattattatt gtaactcaga catccatgat ttttattatt acccaaatag
     1021 ttttctttta tacttattta atttaaattt aatgaagtaa ataaaagcgt taaacgtatc
     1081 accatttttc tttgcatgtt gatatttcac ttgatactgc ttttcattcg tggctggaat
     1141 aaacgccaca tcattccagc aagaagcctg ataagcctca tta
//
LOCUS       DAOFQD010000328         1179 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.336, whole genome shotgun sequence.
ACCESSION   DAOFQD010000328 DAOFQD010000000
VERSION     DAOFQD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.336"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(9..1129)
                     /locus_tag="QBF46_004919"
     CDS             complement(join(9..871,871..1129))
                     /locus_tag="QBF46_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
                     /protein_id="HDQ6676987.1"
                     /translation="MKKRFSDEQIISILREAEAGVPARELCRKHAISDATFYTWRKKY
                     GGMEVPEVKRLKSLEEENTRLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVMLMCD
                     ATGLSQRRACRLTGLSLSTCRYEAHRPAADAHLSGRITELALERRRFGYRRIWQLLRR
                     EGLHVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLRPAAPNLTWSMDFVMDALST
                     GRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTC
                     RALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDW
                     RQDYNECRPHSTLNYQTPSEFAAGWRKGHSENEDSDVTN"
BASE COUNT          279 a          338 c          292 g          270 t
ORIGIN      
        1 tacaacactc agttagtaac gtcggaatct tcattctcag aatgaccctt tctccagccc
       61 gctgcaaatt cagacggtgt ctgataattc agcgtggagt gcgggcggca ttcgttataa
      121 tcctgccgcc agtcattaat aattttcctg gcatgaacga tatcgctgaa ccagtgctca
      181 ttcaaacatt catcgcgaaa tcgtccgtta aagctctcaa taaatccgtt ctgcgttggc
      241 ttgcccggct ggattaagcg caactcaaca ccatgctcaa aggcccattg atccagtgca
      301 cggcaagtga actccggccc ctggtcagtt cttatcgtcg ccggatagcc tcgaaacagt
      361 gcaatgctgt ccagaatacg cgtgacctga acgcctgaaa tcccaaaggc aacagtgacc
      421 gtcaggcatt cctttgtgaa atcatcgacg caggtaagac acttgatcct gcgaccggtg
      481 gaaagtgcgt ccatgacgaa atccatcgac caggtcagat tgggcgccgc cggacggagc
      541 agcggcagac gttctgttgc cagcccttta cgacgtcttc tgcgttttac gcccaggcca
      601 ctgaggtgat aaagccggta cacgcgctta tgattaacat gaagcccttc acggcgcagc
      661 aactgccaaa tacgacggta gccaaaacgc ctgcgctcca gtgccagctc agtgatgcgc
      721 cctgataaat gcgcatcagc agccggacgg tgagcctcat agcggcaggt cgacagggat
      781 aaacctgtaa gcctgcaggc acgacgttgc gacagaccgg tcgcatcaca catcaacatc
      841 acggcttccc gcttctggtc tgtcgtcagt actttcgccc aagagccacc tgaagcgcct
      901 ctttatccag catggcttcg gcaagcagct tcttgagtct ggtgttctct tcctcaagcg
      961 acttcaggcg cttaacttca ggcacctcca taccgccata cttcttacgc caggtgtaaa
     1021 acgtggcatc ggaaatggca tgcttgcggc agagttcacg ggcgggtacc ccagcttcgg
     1081 cttcgcggag aatactgatg atctgttcgt cggaaaaacg cttcttcatg gggatgtcct
     1141 catgtggctt atgaagacat tactaacatc ggggtgtac
//
LOCUS       DAOFQD010000329         1169 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.337, whole genome shotgun sequence.
ACCESSION   DAOFQD010000329 DAOFQD010000000
VERSION     DAOFQD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.337"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            40..438
                     /locus_tag="QBF46_004920"
     CDS             40..438
                     /locus_tag="QBF46_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676988.1"
                     /translation="MEMLGKILVIIISMIIISCDYYNAGMDYQDNIALEQVVTWDSDN
                     IFTPNIFEHNGRCFMKKNETWMLLNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSI
                     TDKERENLKALICSKPAKSVNTGKRPALGY"
     gene            502..1056
                     /locus_tag="QBF46_004921"
                     /pseudo
     CDS             502..1056
                     /locus_tag="QBF46_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          388 a          194 c          276 g          311 t
ORIGIN      
        1 ttcactggat tcccatttga atttcaagag gtaaagtaaa tggaaatgct tgggaagata
       61 ttagtgataa ttatttccat gataattata tcttgcgatt attataatgc tggcatggac
      121 tatcaagata atatagccct cgagcaagtc gtaacatggg attctgataa tatattcaca
      181 cctaatatct ttgaacataa tggaagatgt tttatgaaga aaaatgaaac atggatgtta
      241 ttaaatggag atgcaacacc aaacattaga acaatgagca ataagaaaat tgaatgcctg
      301 gagaagagtg aagtagaatg ggctagtagg gtttgcgaca gtatcacaga caaggaaagg
      361 gaaaatctga aggcattaat atgtagcaaa ccagctaaat cagttaatac aggcaaaaga
      421 ccagcgttag gatattgaaa ccggcactgc actatatcac tattacaata ttctgagtga
      481 ctagagcaac tgctccgcca tccgggctag cagtatgacg acgaatccga actgcactac
      541 aactggcagc ggtactacaa tccgggacag ggacgatata tcacccagga cccgataggg
      601 ctggcggggg gattaaaccc gtacagttat gttatgaaca atcctgtgca atggattgat
      661 ccattgggat tagctcatac ttcaggaaaa tggaaggact gtggtaaagg ttgtcgaatc
      721 cgaatagatg gcgatgctgt tggtgacgga agacatttgc attgggagtg taaaaatggg
      781 agtggtgtaa tgggggaatt tggaggtatg tcccatgatg aggattactc atcagcacca
      841 aatagcatta agaaatgtgc aaaaaaatat ggtttcgagc cagaaccaag taaagatctg
      901 tcatgcaaaa cctccgggct atcaactact gatgcggctc ttgcaactgg tgctacaatt
      961 ggcgcagggt atgttatata tagagtgatt cgatttatac cttcacttgc accacctctt
     1021 tggtggtcaa tacctgcaaa cgttgttact ccatgattaa aacggataat ggagattata
     1081 taatgagcga ttttgacatt gtgagttcaa ttgactggca tgatggggtt ttgttagaag
     1141 taaaaacgac attcaaagaa tctcatttt
//
LOCUS       DAOFQD010000330         1145 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.338, whole genome shotgun sequence.
ACCESSION   DAOFQD010000330 DAOFQD010000000
VERSION     DAOFQD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.338"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            18..404
                     /locus_tag="QBF46_004922"
     CDS             18..404
                     /locus_tag="QBF46_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676989.1"
                     /translation="MNKFIVTSFFIAIFSISGCSTSGNQKIKNETAQSLQSKIIKNKT
                     TKAELVAQLGEPDTRTTLDDGNEQWRYFMYNNQFNASTFIPVVGLLTGGSQTQSKTLE
                     IDFNGETVSKWTFSTDNNNTKSGILN"
     gene            424..741
                     /locus_tag="QBF46_004923"
     CDS             424..741
                     /locus_tag="QBF46_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HDQ6676990.1"
                     /translation="MKTYSILAVILSTFLLTACNSEPSEKDIFNVFKSVVDRSNASMK
                     ALNSSIPEKDLLRIDYVKKVSCTEEANNVYNCIVDASISNMKQTKPVKLVKADGIWKE
                     IQQ"
     gene            complement(796..1026)
                     /locus_tag="QBF46_004924"
     CDS             complement(796..1026)
                     /locus_tag="QBF46_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676991.1"
                     /translation="MAYGKNPFSKSPLPTGDFKTSQKRLTAEIKEVEGKKVIFIYYEG
                     TNDVYDGPEATQYTTLEDAQGFCNNYNKQLNI"
BASE COUNT          394 a          194 c          194 g          363 t
ORIGIN      
        1 ataataagga agataaaatg aataaattta ttgttacatc attttttatt gcaatatttt
       61 caatttctgg ctgttctaca tccggcaacc aaaaaatcaa aaatgaaacg gcacagagtc
      121 tacaatcaaa aataattaaa aacaaaacaa ctaaggcaga gctagttgct caacttggtg
      181 agcctgatac cagaacgact cttgacgatg gcaacgagca atggagatat ttcatgtata
      241 acaatcagtt caatgcctcg acttttatcc ctgttgttgg tttgctaact ggcggttccc
      301 agactcagtc taaaactctc gaaatcgact tcaatggaga aaccgtaagc aaatggacat
      361 tctctactga taataacaat acaaaatctg gcattcttaa ttaatcgttt cctggacaag
      421 aaaatgaaaa catactcaat tctcgcagtt atcctttcta cttttctatt aacagcctgt
      481 aattcagaac cttctgaaaa agatatattt aatgttttta aaagtgttgt agacaggtca
      541 aatgcaagta tgaaagctct gaactcaagt attcccgaaa aagatctttt aagaatcgat
      601 tatgtgaaga aagtaagttg cacagaagaa gcaaacaatg tttataactg cattgtcgat
      661 gcctcgatta gcaatatgaa acagacaaag cctgttaagc ttgtgaaagc tgatggtatc
      721 tggaaagaaa tacaacaata gaaagccata ttgctttaaa gcccctaata gtaggggctt
      781 ttatttgaat attcattaaa tatttagctg tttattataa ttgttacaga aaccttgtgc
      841 atcttcaagt gtagtgtatt gagtcgcttc tggaccatca taaacatcat tagtcccttc
      901 atagtagata aaaatgactt ttttaccttc gacttctttt atctctgcgg taagtctttt
      961 ttgagaggtt ttaaaatcac ctgttgggag tggtgatttt gagaagggat tttttccata
     1021 agccatgagg ttgttctctt ataaaccaaa tgagctataa gtatgatgct atccgttggg
     1081 aaaatcaaac aaaaattaga ttacttaaca atatacaatt ctggtttcat taatcgaagt
     1141 ctttc
//
LOCUS       DAOFQD010000331         1114 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.339, whole genome shotgun sequence.
ACCESSION   DAOFQD010000331 DAOFQD010000000
VERSION     DAOFQD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.339"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..435
                     /locus_tag="QBF46_004925"
     CDS             <1..435
                     /locus_tag="QBF46_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676992.1"
                     /translation="EIDLEEASSDDLAEHLKVLIALWQKHLKTPKPPKRKFRFGHKTF
                     ERILDYKVIPLMDLISWEQLYNEGKNIPFTILADILHGNNGVRSSENIKDTDYDYAKS
                     YLDNDEYFKVLNDFYIKNNMLKDWGIVDVITLNDKSSDKNKK"
     gene            622..837
                     /locus_tag="QBF46_004926"
     CDS             622..837
                     /locus_tag="QBF46_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDQ6676993.1"
                     /translation="MVQNTTDKHPKLIRLPEVKRRTGFGKTWIYTLIRAGKFPKQVKT
                     GLRSIAFIESEIDEWIYKIISDSRNHI"
     gene            867..>1114
                     /locus_tag="QBF46_004927"
     CDS             867..>1114
                     /locus_tag="QBF46_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676994.1"
                     /translation="MKSKNVLPCVVTVTNDETEVFMEMAINNFRKHLQVMIDCMGNDY
                     ERHFKDRLYIEEVIGKVIERTKQEFAESMKDNKGKEYYL"
BASE COUNT          431 a          175 c          215 g          293 t
ORIGIN      
        1 gaaattgact tggaagaagc cagcagtgac gatctcgcag aacatctgaa agtactgatt
       61 gctctatggc aaaagcacct gaaaacgcct aaacccccga aacgaaaatt taggtttggt
      121 cataagacct ttgaaagaat acttgattac aaagtcatcc ctttgatgga tttgatctca
      181 tgggaacagc tctacaacga aggtaagaac atacctttca ccattttagc tgacatcctg
      241 catggtaata atggggtcag aagtagtgag aacatcaaag atactgatta tgattacgcg
      301 aaaagttatc ttgataacga tgagtacttc aaggttttaa atgatttcta tattaaaaac
      361 aatatgttga aggattgggg tatcgttgat gtgataacac ttaacgataa atcctcggat
      421 aaaaacaaaa agtaaacaca agggatgttc acaacctgcg aacatcccta aaaagcgtat
      481 ccctccaaaa atttccgcga aactcacggt tggtgaacat ccctagttta gaactagcca
      541 aaatcctaga attatctcca tcgaacaaca tactaaaaaa cgaaatatcc aagatattac
      601 gtaaaaaaaa caggagataa tatggttcaa aacacaacag ataaacatcc gaaactgatt
      661 cgtttgccag aagtaaaaag aagaacaggg tttggtaaaa cgtggatata taccttaata
      721 agagcgggta aatttcctaa gcaagtaaaa accggattgc gctctatagc atttattgaa
      781 agtgagattg atgaatggat ttacaaaatc atttctgatt ctcgcaatca tatctaaaaa
      841 agtttattaa ataatgagag gttaagatga aaagtaaaaa cgtactgcct tgtgtggtga
      901 ctgttacgaa tgatgagaca gaagtattta tggaaatggc aataaataac tttagaaaac
      961 atcttcaggt catgattgat tgtatgggaa atgattatga gcgtcatttt aaagaccgac
     1021 tttatattga agaagtgatt ggaaaagtga tcgaacgaac aaaacaagag tttgctgaat
     1081 caatgaaaga taataaaggg aaagaatatt atct
//
LOCUS       DAOFQD010000332         1082 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.341, whole genome shotgun sequence.
ACCESSION   DAOFQD010000332 DAOFQD010000000
VERSION     DAOFQD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.341"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          345 a          187 c          208 g          342 t
ORIGIN      
        1 gtctcctgtg aaaaatctct ttccacatta tttaaataaa cagagagcca gattaaatac
       61 ctgagtataa aatctattct gatgtttaat tgatttgaat gttcgtaagc tatatcactt
      121 actcaatcca ttttacccgg agtcataaaa tagagtgtct gttgatctca ttttcatatt
      181 cctttcagac tgaagtgcgt tttcatcacc cgatgagaaa gaactgaagt gatgtttgcc
      241 atagattacg agatgcccag tctcattgac tatcgtggtg cacctttata cctgttatac
      301 cagatcaaaa atcacgcaat ccatacaaca aaaccagatt tgcaattcgt gtcacaaaat
      361 atgtcgatct ttttctaaga agaagatgcc atatgaagcc aaacgaacac ttgcggaggc
      421 catcacaaac caaagattgt tgtttaatta tatgttttat aagtaattta tttaaagtat
      481 aaacaaggaa agatttgcaa gctaactcag tgggtgtggt gaaaataagt gtttacccgc
      541 catcaggctg agcataattc tcatcatgaa atatgtttcc tggtttgtgg cttgtaactg
      601 gtcacttctg aagtcgatct ggagaggctt gttgatgctg gtgttttcag gatgatgttt
      661 cagatagttt gtttgccgta tcgcccggcg aatagctgtg attgaggaag gtttaagtcg
      721 tagtaaccaa agccatattt accaacgaat gtagatgaaa aaatcatctc ctgcgttccc
      781 ccatatctcc aggataaaaa ggaatgtaac aatctcatgg cctaaactga cgaaccagcc
      841 aggaataatc gctagggacc taagaattag catgataata gccactaaga aattactgcg
      901 ttccatgaaa tagccatttt gtggcaaatg gagttgacta ataatgtcat atgtgagacg
      961 gctagttgaa cgaatattaa attttgctga attttttatg ttgattttac ttgttacaga
     1021 acatatcaca tgatatatag ataagattag ttgcattaat gatgagggtt attattagat
     1081 tc
//
LOCUS       DAOFQD010000333         1073 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.342, whole genome shotgun sequence.
ACCESSION   DAOFQD010000333 DAOFQD010000000
VERSION     DAOFQD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.342"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(287..922)
                     /locus_tag="QBF46_004928"
     CDS             complement(287..922)
                     /locus_tag="QBF46_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDQ6676995.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          275 a          232 c          227 g          339 t
ORIGIN      
        1 gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa
       61 gcaagggtat gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg
      121 tgatgacacg ggattattgc ggtttacttt gaggaatata aaatgcgagt ttatagcacc
      181 gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg
      241 gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca
      301 gtgcttcgct atcgacgatg attccatctt ccgcaccttt aagcgccagg tttgcctcgt
      361 agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa
      421 gtggcatcag atattggttt tccattacgc caacaccggc caggcgaagc tcttccattg
      481 cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt
      541 cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt
      601 catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat
      661 ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa
      721 acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac
      781 gctctgctgc cgctgccgtt ttctgggttt gctgaacttg ctgagtttgc tgttgtgccg
      841 ctaccggagc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
      901 tggccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
      961 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaaggattg
     1021 tcttacagtt gtagttatta tcgcaagaag tattttagtc ttacaattgt agg
//
LOCUS       DAOFQD010000334         1068 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.343, whole genome shotgun sequence.
ACCESSION   DAOFQD010000334 DAOFQD010000000
VERSION     DAOFQD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.343"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..513)
                     /locus_tag="QBF46_004929"
     CDS             complement(<1..513)
                     /locus_tag="QBF46_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDQ6676996.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYL"
     gene            782..982
                     /gene="glgS"
                     /locus_tag="QBF46_004930"
     CDS             782..982
                     /gene="glgS"
                     /locus_tag="QBF46_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDQ6676997.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          333 a          189 c          249 g          297 t
ORIGIN      
        1 taaataatgt atttggccaa attgatcggt gagttttcct tcgcagggat tacctgaagt
       61 ggcttgatga ccggtaatta acgccatgct accaatatat tcttcttctg taatagctga
      121 actatggtcg cgtggaatcc agggcgcaac tattttaccg gtataatgca ccgcaataat
      181 cgtaaacagt aaactgacag gaaccacgaa caggttcgac agtggtgatt gccagaccat
      241 gatgcaggca tgctgcaaca ggatgccaat aagaccaaaa aaaccagcca gtaagcagag
      301 aacgacgaga gctggcaatc ttccaatatt gagataatga agtgcctggc taatatgacc
      361 tgtggttatg gaatcataat ggtcaagatg tgcatcaaga gcaccggaga gcatgtgacc
      421 gcagataagc gcaaaaattt ctaacaggcc gatcaacagt acaaaggaaa tagcgaaaag
      481 ataaggggtg ttatagtcgg cgaataaaat catatatcac tccaatgtcc atttagtgac
      541 gcaatattta ctttgtaata gtttccttgc ttacatttat ctgtgttaat aataacacag
      601 gttagagaag tactggaaga gaaagtcttt taatatggtt ttttattttt cactaaaaag
      661 tgtgatcggg gacaatatat ttacgcacgt tatgtttaaa ggcactacac tgattgggga
      721 atactgaaat cagaaaaagc atatggcctt aaaaggtatg cgataaccgg aggatgtgct
      781 tatggatcat agtcttaatt ctttaaataa tttcgatttc ctggcgcgta gttttgccag
      841 aatgcacgca gaaggtcgcc cggtcgatat tctggccgtt actggtaaca tggatgaaga
      901 acatagaacc tggttttgcg cacgttatgc ctggtattgt caacagatga tgcaggcaag
      961 agagctggag ttagagcact gatataacgg gcctgatggc ccgttttagt gtttgtatta
     1021 gatactagtt ataggtgatt ttcaggatta cgtctttact aaacggtc
//
LOCUS       DAOFQD010000335         1063 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.344, whole genome shotgun sequence.
ACCESSION   DAOFQD010000335 DAOFQD010000000
VERSION     DAOFQD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.344"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..208
                     /locus_tag="QBF46_004931"
     CDS             <1..208
                     /locus_tag="QBF46_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6676998.1"
                     /translation="TLDDGNEQWKYFMSNNQFSATTFIPVVGLFTGGSQTQSKTLEID
                     FKGETVSKWTFSSDNNNTKTGILN"
     gene            227..541
                     /locus_tag="QBF46_004932"
     CDS             227..541
                     /locus_tag="QBF46_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HDQ6676999.1"
                     /translation="MKIYSILTVFLTTLLLTACNSEPSQDDIYNAFKSVVDRSNASMK
                     ALNSSIPEKDLLKIDYVKKVSCTEEANNVYNCIVDASISNNKQTKPIKLVKADGVWKE
                     VQ"
     gene            complement(591..1016)
                     /locus_tag="QBF46_004933"
     CDS             complement(591..1016)
                     /locus_tag="QBF46_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677000.1"
                     /translation="MDKNIIYPEFTLEEQLIIIVDKYISKRYQLGDKNFSYQLYLIFV
                     GYHLKYFYPKRIYSKSNRNIDNIMAMFSSVYKSLTSNLLQLLNNKEGVLRELNSLVNF
                     IDNNQEKAEEIYAVVRTQYEVKVIEKELTHEVRIRTVRL"
BASE COUNT          342 a          198 c          180 g          343 t
ORIGIN      
        1 cacactcgat gacggaaatg agcagtggaa atatttcatg tctaacaatc agttcagtgc
       61 aacgactttc attccagttg tggggctgtt tactggcggt tctcagactc agtctaaaac
      121 tcttgagatc gacttcaagg gagagaccgt aagtaaatgg actttctcct ctgataacaa
      181 caacaccaag actggtattc ttaattaatt atttctggat aagaaaatga aaatatactc
      241 aatcctcaca gttttcctta ccacacttct tttaaccgca tgtaacagtg agccatccca
      301 agacgatata tacaatgctt tcaaaagtgt cgtagataga tcaaatgcaa gtatgaaagc
      361 tctgaactca agtattcccg aaaaagacct tttgaaaatt gattatgtaa aaaaagttag
      421 ttgcacagaa gaagcaaata atgtttataa ctgcattgta gatgcttcta ttagcaacaa
      481 taaacagaca aagccaatta aattagttaa agcagatggt gtatggaaag aagttcagta
      541 acagccgcta ccaaacactc tctgttagcc cctacttagg ggctatttta ctacaaccta
      601 acggttctta tgcgaacctc gtgagttaac tctttctcga taaccttcac ttcatactgt
      661 gttcttacga cagcatagat ttcttctgct ttttcctgat tgttatctat gaaattcaca
      721 agcgagttta gctctcttag gaccccttct ttgttattta atagttgtaa tagattgctt
      781 gtcagacttt tgtaaaccga gctaaacata gccattatgt tatcaatatt acgattggat
      841 ttggaataaa tccttttagg gtagaaatat tttaaatggt atcccacaaa aattaagtaa
      901 agctggtatg agaaattttt atcacctagc tggtatcgtt ttgagatata tttatcgacg
      961 ataatgataa gttgctcttc cagagtaaac tctggataga taatgttctt gtccataaaa
     1021 tgcatccttg cattagttaa atagtcttcc tgaccatata ttt
//
LOCUS       DAOFQD010000336         1056 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.345, whole genome shotgun sequence.
ACCESSION   DAOFQD010000336 DAOFQD010000000
VERSION     DAOFQD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.345"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..938)
                     /locus_tag="QBF46_004934"
     CDS             complement(<1..938)
                     /locus_tag="QBF46_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6677001.1"
                     /translation="MSKISGWNFSQNITLADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASVFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDN
                     EINDIKVNLSHILIDSLDDAKVNLTPVIDSGDFLKFP"
BASE COUNT          329 a          210 c          161 g          356 t
ORIGIN      
        1 gggaatttta aaaagtctcc agaatcgatg accggtgtaa ggttaacttt tgcatcatct
       61 aaggaatcaa taagaatatg agacaggtta actttgatgt cattaatttc attgtcagta
      121 gctttcttat tattaaggag ataattattt actgcgttaa ggtcatctat ttctttcagt
      181 atcccttttt caccaagcaa gtatgaaatc gattcattac atacataagg cattaatagc
      241 agatgttctt tccttattgc taataatgca ttattcaatg gtttttctaa taaatccgta
      301 atcgtagaat caactgaatc tttacaactc acctgcgagt tattcaagtc ctgagaatta
      361 tttaactcct gcggaatatt taatagacga gctatttcct tgttagcttc gcgttggtaa
      421 cacgccagaa tcttgttagt aatcaggtgt aatgaactct catcttttct ttcaaaggac
      481 ttcccttcat aacgattagt cgaaccatca ggttgtgtaa atataatgac tgtatgtcct
      541 tcacgaatcc cattattaaa aactgaggca agtgtaattt ctatttccac cttagataaa
      601 tccgctcgct ccccttcagg attattcata acaaacttca ttatctcact accatcatta
      661 tttttgatat aagatgagga cttaatatga gccatcatat ttctacccgc ttcgttggag
      721 cgtttgctaa aatccacata acccagggta aatatcttag ccaaaaactc caaaaatgct
      781 ctgggagaaa gaatattgct tctgctattc tgccctccgg caattcgggc aaaatcattc
      841 attcccacat accaggtatc taagtcttca tttttttgtt tacaattgtc ggctaatgta
      901 atgttttgag aaaaattcca acctgatatt ttgctcatga cggactcctt gagttaataa
      961 gaacgtctaa gcattacaaa ttttactcac aaaaaaatat caaaaaacgc tcttttcaca
     1021 aactcgccgt aaacctggag ttatttacgg cgaaaa
//
LOCUS       DAOFQD010000337         1054 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.346, whole genome shotgun sequence.
ACCESSION   DAOFQD010000337 DAOFQD010000000
VERSION     DAOFQD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.346"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..834
                     /locus_tag="QBF46_004935"
     CDS             <1..834
                     /locus_tag="QBF46_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677002.1"
                     /translation="ALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQP
                     LVAGFLSLIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISV
                     AKKVKYKSLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGV
                     VLAINLFFLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMW
                     ITLVSVLATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFN
                     K"
BASE COUNT          231 a          198 c          259 g          366 t
ORIGIN      
        1 gccctggtgc tgtataattt tatttttgat ctcaaggcgt actgggctta taaatgcgtc
       61 acgaagaata tcgatttttc gtggtttaag aaaaagcaga accacaaaat agaattattt
      121 cttacgcaac ctctggtggc aggatttctg tcgttaatca tgttgagtgc aatgagttgg
      181 gggctatacc agcttctacc ctcgttatat gcgctgttcc tgatttcgtt acttgggccg
      241 ttggttatct ttctgctgtt tcggatgatc cgcaccagtt atgtcaagca ggtcgctatt
      301 tcagtagcga aaaaagtaaa atataaaagt ctgactcgct atgtgctgct ttcggtgtgc
      361 atctcaacgg ttgttaacct gcttactatc agcccgttgc gtaacagtga ttcttttgtg
      421 atagaggggc agtggttaac gtttaaatcg ataattgcat tgctcattct ttgtggcgta
      481 gtgttggcga ttaatctgtt ttttctgcgc ttctccaggc ggtacgcttt tttgggcagg
      541 ctttttttgc aggaaatcga tctctttttc tccagtgaaa atgcgttgtc gacctttttt
      601 gccaagccgc tctggcttcg gttattcata ttgctggtta ttgaagtgat gtggattacg
      661 ctggtgtcgg tattggcaac gcttgtggaa tggcggattt ggtttgaagc ctatttttta
      721 ctctgctatg taccgtgctt aatttactat tttttctatt gtcgattcct ctggcataac
      781 gattttatga tggcatgtga catgtatttc cgttgggggc attttaataa gtgaggaagt
      841 gataggaagt gaccagataa tacatatatg ttctgtactc tcttgcgcat tttgattgtt
      901 gactgagtaa ccagacagtt gatgtgcacg atttcccctc gccctaacag acgtgggcgg
      961 gggcacccgg tgtgtgcaat atacgaaaaa aaagcccgta ctttcgtacg agctcttctt
     1021 taaagatggc ggtgaggggg ggattgactc gctt
//
LOCUS       DAOFQD010000338         1048 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.347, whole genome shotgun sequence.
ACCESSION   DAOFQD010000338 DAOFQD010000000
VERSION     DAOFQD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.347"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          265 a          269 c          239 g          275 t
ORIGIN      
        1 tatagaggca gttatgtaaa accactcatt agcctcaaaa caaagacaaa cttccagatt
       61 tattacttaa ccaatgaata acaagcttaa aaaatatact gttattctaa agaaaaaatt
      121 aacaggcatt aattcaatat taaaaaaata atagattaaa atttcttaac gatttaagaa
      181 tcatacaaat aacactttga ttaaatttaa ttttttgtat cgataattgt gaacctctct
      241 ggcatggaga actattttga acatgaggtg ttacgtggat atgttgctta ttacaagtac
      301 tgctaatata aaaacttgag aaagagataa cgggttatat ggtggtttat ccccgctggc
      361 gcggggaact catatacagc aaaccttcag aatgcattcc gggcggttta tccccgctgg
      421 cgcggggaac tcgccgtctg cctccagccc agccctggat atttcggttt atccccgctg
      481 gcgcggggaa ctctggttca ctcacgcaac gtctgagcgc gttgacggtt tatccccact
      541 ggcgcgggga actcagggcc gccgctaccc cagaagtgcc actccccggt ttatccccgc
      601 tggcgcgggg aactcgcgta tcgtctcgtt attgcgccgc cccaactcgg tttatccccg
      661 ctggcgcggg gaactcggcg tttttgactg tacgaatccc tgcgccgccg gtttatcccc
      721 gctggcgcgg ggaactccac gacgggcagg cgatcaccgg catgacccac ggtttatccc
      781 cgctggcgcg gggaaactct ctaatccccc acgtagacgc ccacgcggtc gcggtttatc
      841 cccgctggcg cggggaactc gtgatcggat atatcccaca ttgtgaatat tgcggtttat
      901 ccccgctggc gcggggaact catcccctaa atttatttct ggttggggcg tatcggttta
      961 tccccgctgg cgcggggaac tctgtaaaca taacctatta ttaattaatg attttttaag
     1021 ccagtcacaa tctaccaact ttacagta
//
LOCUS       DAOFQD010000339         1039 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.348, whole genome shotgun sequence.
ACCESSION   DAOFQD010000339 DAOFQD010000000
VERSION     DAOFQD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.348"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(137..709)
                     /locus_tag="QBF46_004936"
     CDS             complement(137..709)
                     /locus_tag="QBF46_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HDQ6677003.1"
                     /translation="MKLRLWNLLPHDYAPFFRILHIIVAFLILSQIINSNLTETEAIG
                     EHSLEGVITWMHIISGLGLIICGFIMLSWMLTQRGFTYYFSWVGLDFSGIKQDIKTLT
                     SFRLPDAHSGGIASTIQGFGVLALLIVALSGGLWFLLNTMQSNLAETVIHWHKFFTTF
                     IEVYFYAHGAMGVLHILIEKYKSRSVNLSD"
BASE COUNT          347 a          189 c          163 g          340 t
ORIGIN      
        1 catttcgcca tgaatatttt ttcgttttta ttaaatttaa ttaaaaagtc tctgcaagtg
       61 ctgtttcatc ttctagtaaa ctaaatttta tttttagggt tttcttcaac ttgaagtaat
      121 attatctcac aacagattag tcacttaggt taactgagcg gcttttatat ttttcaatta
      181 aaatatgcaa aactcccatt gcaccatgtg cataaaaata gacctcaatg aaagtagtaa
      241 agaacttatg ccagtggatt actgtttcag ccagatttga ctgcatggta ttcaacaaga
      301 accataggcc acctgaaagt gctacaatta gcaatgctag aactccaaat ccttggattg
      361 tactggcaat acctcccgaa tgtgcatcgg gcagtcggaa actagtcaac gtttttatat
      421 cttgcttaat accactaaag tcaagcccta cccatgaaaa ataataagta aaacctcttt
      481 gagtaagcat ccaacttaac ataataaaac cacaaataat cagtcctaac cctgaaataa
      541 tgtgcatcca ggttataact ccttcaagac tatgttcacc aattgcttcg gtctctgtca
      601 gattagagtt aataatttgt gataatatta gaaatgccac aatgatgtga agaatacgaa
      661 aaaaaggagc gtaatcatgg ggtaatagat tccagagtcg caatttcatt aatgagtcct
      721 tagtaaattt atcgatgatc ttaatcatac aaaagaatta ttaggataac cttaattctt
      781 aaaattgcag caggcattag ttcacctctg atatggccac ttgcgaaatg ggaatggtaa
      841 ctgttattac tatgtgttta cttgctgttc ttgttacgca gccccttaaa cacattactt
      901 tcaaatatga tttcctgctg ggtttaaata tgtgtctttc tatagtgcgg caacctattt
      961 ttacaccaac tcatgaatta aataagaatc ataaattaaa tgagaggttt aattctcttg
     1021 cttaacgcga tgatattaa
//
LOCUS       DAOFQD010000340         1039 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.349, whole genome shotgun sequence.
ACCESSION   DAOFQD010000340 DAOFQD010000000
VERSION     DAOFQD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.349"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..199
                     /locus_tag="QBF46_004937"
     CDS             <1..199
                     /locus_tag="QBF46_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6677004.1"
                     /translation="HLIECDNETPDINSVDRIRLQSVREKVMQLKRITQIAYENGDYG
                     ADKWLQHHEKNLERINKVLNN"
     gene            214..600
                     /locus_tag="QBF46_004938"
     CDS             214..600
                     /locus_tag="QBF46_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677005.1"
                     /translation="MAKHLNRSEIKAIKHIILTWDGKITWSDLCESVYKNLNRTITRQ
                     SLSAHDEVVEAYRIKKSLSNLKKSGLKKPANLTIAAQQIINLKAENEMLKKQNNRYKE
                     QFSYWQYNAYRHGLTMEQLNRPFNKK"
     gene            696..>1039
                     /locus_tag="QBF46_004939"
     CDS             696..>1039
                     /locus_tag="QBF46_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677006.1"
                     /translation="MDKNIINLEFTLEEQLIIIVDKYISKRYQPGDKSFSYQLYLLFV
                     GYHLKYFYPRQLYIRSNRNIDNIMTMFSSVYKCLTSTLLQRLNNKEAVIRELNSLVNY
                     IDNNQEKAEEIYT"
BASE COUNT          417 a          158 c          181 g          283 t
ORIGIN      
        1 tcatcttatt gaatgtgata atgaaacacc ggacattaac tcagtagata gaattagact
       61 gcaatctgtc cgagagaaag taatgcaatt aaagagaata actcagatcg cttatgaaaa
      121 tggtgattat ggtgcagaca aatggttgca acaccatgaa aaaaatttgg aaagaattaa
      181 taaggttttg aataattaaa acaggagata cttatggcaa aacatttgaa caggagtgaa
      241 ataaaggcaa ttaaacacat tatactgact tgggacggta aaatcacatg gagtgattta
      301 tgtgaatcag tgtataagaa tctaaacaga actatcacaa ggcaatcttt aagtgctcat
      361 gatgaagttg ttgaagcata tagaataaag aaaagtttgt ccaatttgaa aaaatctggt
      421 ctaaagaaac cagctaactt aactattgcg gcacaacaaa taataaacct taaagccgaa
      481 aacgaaatgt tgaaaaaaca gaacaataga tacaaagagc aatttagcta ttggcagtat
      541 aatgcttaca gacatggtct cactatggaa caattgaata gaccatttaa taaaaaataa
      601 tagttgatag gttacaccta ttgacaagtt aaataaatga gataaatata aacatatggt
      661 caggaagact atttcattat gcaaggatgc attttatgga caaaaacatc atcaatctag
      721 agtttactct agaagagcaa cttatcatta ttgtcgataa gtatatctca aaaagatacc
      781 agccaggcga taaaagtttc tcataccagc tatacctgct ttttgttggc tatcatttaa
      841 agtatttcta cccaagacag ctatacattc gctctaatcg taacatagac aacattatga
      901 cgatgttcag ttctgtatac aaatgcctta caagtacttt gttgcagcga ttgaacaata
      961 aggaggctgt tatcagagag ctaaactctc tcgtgaacta tattgacaac aatcaagaaa
     1021 aagcagaaga aatctatac
//
LOCUS       DAOFQD010000341         1018 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.350, whole genome shotgun sequence.
ACCESSION   DAOFQD010000341 DAOFQD010000000
VERSION     DAOFQD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.350"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>1018
                     /locus_tag="QBF46_004940"
     CDS             <1..>1018
                     /locus_tag="QBF46_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6677007.1"
                     /translation="VQYYFNSNSYLYVKNIVRNMESLIRKLSPTHIDDKVLIEYQNKK
                     LSKAPASFRVLRPFLIKWFELGYPGIDESAVELLKHLDLKIKKSGQSVLQDDPTEGPL
                     TKEEHTSLIKAMNHAYRKGELSLPHYAISLLISLTGRRPQQLVMLKYKDLLQKNLDNG
                     KVEYVISVPRVKQRGKELRYRELAIISEVASIVQLQANQSVKLVEQALGKTLDDYSKR
                     EVPIFLNEEKLSDLSITGSILLEYNKLYAKPIIANVALKGIVNNGNIISNRTGSIINI
                     TPRRLRYTIATMLAKDGHNVNTIAELLDHSSTSSAGIYIKNHADSVERIDSAVSEQLS
                     FVAD"
BASE COUNT          356 a          160 c          187 g          315 t
ORIGIN      
        1 cagtacaata ttattttaat agcaacagct atttgtatgt gaaaaatatt gttagaaata
       61 tggagagtct tatccgtaaa ctatctccta ctcatattga tgataaggtt ctaattgaat
      121 atcaaaataa gaaattatca aaagccccgg catcatttcg cgttttacga ccatttttga
      181 ttaaatggtt tgaattagga tatccaggaa tagatgaaag tgcggtagag ctgttaaagc
      241 atttggacct aaagataaaa aaatctggtc agtctgtgct tcaagatgat ccaacagagg
      301 gaccactaac taaagaagaa catacttctt tgattaaggc tatgaatcat gcatatagaa
      361 aaggtgaact gtcactgcca cattatgcaa tatcactatt gatcagcctt acaggtagaa
      421 gacctcagca gttagttatg ttgaaatata aagaccttct tcaaaagaac ttagataatg
      481 gcaaagtaga atatgtaatt tcagtgccac gagttaaaca acggggtaaa gaactacgat
      541 atcgagaact tgcaataata tcagaggttg catcaattgt tcaattgcaa gctaatcaat
      601 cagtgaaact tgttgagcaa gctcttggaa aaactcttga tgattattct aaacgagaag
      661 ttcctatttt cttaaatgag gaaaaactct cagatttatc cataacaggt tcaattcttc
      721 tggaatataa taaattatat gcgaagccta taattgctaa tgtagcattg aaaggtattg
      781 ttaataatgg gaatataata tccaaccgta ccggttcgat aataaatatt actcctcgca
      841 ggcttcgtta tacaatagca actatgctgg ctaaggatgg gcataatgtt aatactatag
      901 ctgagttatt ggatcattcc tctacttcaa gtgcgggtat ttatattaaa aatcatgctg
      961 acagtgttga aaggattgat tccgctgttt cagaacaatt gtcatttgtc gctgatat
//
LOCUS       DAOFQD010000342         1015 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.351, whole genome shotgun sequence.
ACCESSION   DAOFQD010000342 DAOFQD010000000
VERSION     DAOFQD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.351"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..274
                     /locus_tag="QBF46_004941"
     CDS             <1..274
                     /locus_tag="QBF46_004941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6677008.1"
                     /translation="YLTLKKIYHLQNKKINNSDIAFVKNSFSMGVMDEAKEALRLLGG
                     WSIKSKMPDLYAKRFMSEQANAVNIKRLLQQDSELDCLMKGLFDGY"
     gene            264..>1015
                     /locus_tag="QBF46_004942"
     CDS             264..>1015
                     /locus_tag="QBF46_004942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6677009.1"
                     /translation="MGIKSKILFHQSSGMYERDLSSKIKSLVIPKRIFLKSSNDFDDT
                     HIHNEGDRWVFYFSGSRRYVKFNLDEMSNAILKYFCFKYASTMFPTFLPVLSHQWAKA
                     LSYCREKGGFTLIILRKYIEGDILDARAFYSILFGLKILCCEEFPGFTLEDYEDLEFI
                     PRPNAFNWEIYQDIDNILEPLEKSMITKGLFEIATGLARGKTYDIKELKHTAVLALSY
                     ATGARPVQLAKLSVRDLRIDTCDTHTGLIRYS"
BASE COUNT          326 a          149 c          210 g          330 t
ORIGIN      
        1 ttacttaact ttaaaaaaaa tttatcattt gcagaataaa aaaatcaata actctgatat
       61 tgctttcgta aaaaactcat tttctatggg agtgatggac gaagcgaaag aggcgcttcg
      121 tttacttggc ggttggagta taaaaagtaa aatgcctgac ctttacgcaa aaaggtttat
      181 gtctgagcaa gccaatgcag taaatattaa gagattactg caacaagatt ctgaactcga
      241 ttgcttaatg aaggggttgt ttgatgggta ttaaatctaa aatattgttc catcaaagtt
      301 ctgggatgta tgagcgggat ctctcttcaa aaataaaatc attagttatt cctaaaagga
      361 tttttttgaa atccagcaac gattttgatg atacgcatat tcataatgag ggagatagat
      421 gggtttttta tttttctgga agtcggcgat atgttaagtt taatcttgat gaaatgtcaa
      481 atgcaatatt aaaatatttt tgcttcaaat atgcatcaac tatgttccca actttcttgc
      541 cagtattgtc acatcagtgg gctaaagcgt taagttactg ccgggaaaaa ggcggcttta
      601 cattaattat tttgcgcaag tatatagagg gcgatattct cgatgccaga gctttctatt
      661 ctatattatt tggattgaag atcctttgtt gtgaagagtt ccctggattt actttggaag
      721 actatgagga tcttgagttt atacctagac caaatgcgtt taactgggag atttatcagg
      781 acattgacaa tattctcgaa cctttggaaa aaagcatgat aactaaaggt ttatttgaga
      841 tagcaactgg attggccaga ggtaaaactt atgatattaa agagttaaaa cataccgcag
      901 tactggcgtt aagttatgcc actggagcgc gtcccgtgca gttggcaaag ttatctgttc
      961 gagacttgag aattgataca tgtgatacac atactgggct aattagatat agcat
//
LOCUS       DAOFQD010000343         1002 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.352, whole genome shotgun sequence.
ACCESSION   DAOFQD010000343 DAOFQD010000000
VERSION     DAOFQD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.352"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..588)
                     /gene="dcuC"
                     /locus_tag="QBF46_004943"
     CDS             complement(<1..588)
                     /gene="dcuC"
                     /locus_tag="QBF46_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HDQ6677010.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFA"
BASE COUNT          320 a          216 c          211 g          255 t
ORIGIN      
        1 tgcgaagtca atcagcgaca tttcggaagc ttgcgccgcc agcaccacat cccctgaagt
       61 cggtgcgaga ataatcgccg ccggggaggc acaaatggca gcagctgcgc cacgactgat
      121 accaacgttt accatcaccg gaaatagggt tgccatcagc aaaacaccca gaccggttgc
      181 ggaagagacg gccagagaca tcagacaggc gacaaaatag gcggcaatca tcagcaggta
      241 aggggagtta atatactgca atggttttga cgccagcttg accaccatat cattcgcgcc
      301 gatatgggtc atgtaagcgg caaatccaca cagcatcata atcatcatgc cgaggtcgcc
      361 gccgcggctc attagtaata ttttaacgta ttcaacgata tccgtggcgc tgtagcctgt
      421 tgaagcctgg ctggacggta acactttgtg ccccataatg gcactgataa tcagcaataa
      481 caggccaccg acaaataaca caccagtggc ggaataccct ttaatgatgt agcgagctac
      541 acccacaata accacaaccc caataaggag ttcaatgaat gtcagcataa tttttcctgt
      601 ctccaggccc caaagtaaat aataaaaaat tcctaaagct taaggaaaaa atatgcccaa
      661 taaattggcg atgaatgctg attaaaatca agaaaaactg ccattaagac attgaagttg
      721 ctgtttttat acatagataa caaaacgcat atttagcggt tggcaattat cacataataa
      781 tgtgtgtaaa ttgatggtgg attgatgcaa atttgttaat tgcaatggtg ttagctgcgg
      841 ctgtgcgctc aaaaaataat ctaacatgag cataggttga cgatttatat tatatacttc
      901 gttaagatga ttgttgtatc tagttaaaaa atagaataat ttttccttga ttgcattttg
      961 tcatcaaaaa agacttggtt tttctttttt gactattccc at
//
LOCUS       DAOFQD010000344         1001 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.353, whole genome shotgun sequence.
ACCESSION   DAOFQD010000344 DAOFQD010000000
VERSION     DAOFQD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.353"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..420
                     /gene="yfdX1"
                     /locus_tag="QBF46_004944"
     CDS             <1..420
                     /gene="yfdX1"
                     /locus_tag="QBF46_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance protein YfdX1"
                     /protein_id="HDQ6677011.1"
                     /translation="IETDNLPMATYPAAIKSAARLVDSGKIDEAKAELARALNTLVVT
                     QVVLPLPVLRAEAAIAKAEKLAETDKRDAKQNEELSTLLSSVRTEIELAQILGYGKKE
                     DFKPIFDQVKSIEQKSAGGKSGNGWFDELKTRIQKLF"
     gene            523..>1001
                     /gene="yfdX2"
                     /locus_tag="QBF46_004945"
     CDS             523..>1001
                     /gene="yfdX2"
                     /locus_tag="QBF46_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat resistance protein YfdX2"
                     /protein_id="HDQ6677012.1"
                     /translation="MNEQTSNPNAINEGTNEQAAVSSLPARPEPKPEVVTEVQPEVQK
                     ETDSQAADKRKQVLDEAVSALALTKSALAALDEKESARALATLAEVTGKLELIVAREP
                     TLALAPVDVRTIVHDLFANTETIEAMTDEALDALKHGEVQQARHVLALLASEIVITVT
                     "
BASE COUNT          256 a          293 c          290 g          162 t
ORIGIN      
        1 atcgaaaccg acaacctgcc catggcaacg tacccggcag cgatcaagtc ggctgcacgg
       61 ctcgtcgaca gcggcaagat cgacgaagcc aaagcagagc tcgcccgagc actgaacacg
      121 ctggtggtca cccaagttgt actccctctg cccgtgctac gggccgaagc cgcgatagca
      181 aaagctgaaa agctggccga gactgacaag cgcgatgcca agcagaacga ggagctcagc
      241 accttgctgt cgtccgtgcg cacggagatc gagctggcgc aaatcctggg ttatggcaag
      301 aaggaggact tcaaacccat cttcgatcag gtgaagtcca ttgagcaaaa gtcggctggt
      361 ggcaaaagcg gcaatggatg gttcgacgag ttgaagacgc gcatccaaaa gctgttttga
      421 ggtgatgaag gggccgactg ctccatcggt cagtctcgcg atattcgcag tcggccccaa
      481 cagattcccc tattttcact atctcatacg aggcatatca ccatgaatga gcaaacatcg
      541 aatcccaacg cgatcaacga aggcacgaac gaacaggccg cggtaagcag ccttcctgcc
      601 agaccagagc ccaagcctga agtcgtcacg gaggtacagc ctgaggttca gaaggaaacg
      661 gactcgcagg cggcagacaa acgtaaacaa gtcctcgatg aggccgtctc ggccttggcg
      721 ctgaccaaat cagcgctggc ggccctggac gaaaaagaat ccgcgcgagc actggcaacg
      781 ctggccgaag tgacgggaaa gctggagctg atcgttgcac gcgaacccac gctcgccctg
      841 gcccccgttg atgtgcgcac catcgtgcac gacttgttcg ccaacacgga aaccattgag
      901 gcgatgaccg acgaggcgct ggatgccctc aaacatggcg aggtgcaaca ggctcgccac
      961 gtgctggctt tgctggccag tgaaatcgtg atcacggtca c
//
LOCUS       DAOFQD010000345          946 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.355, whole genome shotgun sequence.
ACCESSION   DAOFQD010000345 DAOFQD010000000
VERSION     DAOFQD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.355"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            197..799
                     /gene="fimB"
                     /locus_tag="QBF46_004946"
     CDS             197..799
                     /gene="fimB"
                     /locus_tag="QBF46_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDQ6677013.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANNGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          288 a          172 c          198 g          288 t
ORIGIN      
        1 aagagcatag cgtttgtatg gcaacgttat tataattaac tgttgctact ccatttaagt
       61 tcactcagaa gaactggtcc acttacgtta gttattaagc aaacgttcgc ttttataaac
      121 ataatcagga taaaaatgtt ggattattgc taacccagca cagctagtgc gcgtctgtaa
      181 ttataaggga aaaacgatga agaataaggc tgataacaaa aaaaggaact tcctgaccca
      241 tagtgaaatc gaatcactcc ttaaagcagc aaataacggg cctcatgcag cacgtaatta
      301 ttgtctgact ttgctttgtt ttattcatgg tttccgggcg agtgaaattt gtcgattgag
      361 gatttcggat attgatctta aggcaaagtg tatatatatc catcgattaa aaaaaggctt
      421 ttcaacaacg cacccgctat tgaacaaaga agttcaggct ttaaaaaact ggttgagtat
      481 ccgtacttcg tacccgcatg ctgagagcga gtgggtattt ttatcgcgta aggggaatcc
      541 gctttctcgg caacagtttt accatattat ctcgacttcc ggtggtaatg ccgggttgtc
      601 actggagatt catccgcaca tgttacgcca ttcgtgtggt tttgctttgg cgaatatggg
      661 aatagatacg cgacttatcc aggattatct tgggcatcgc aatattcgtc atactgtctg
      721 gtataccgcc agcaatgcag ggcgttttta cggcatctgg gatagagcca gaggacgaca
      781 gcgtcacgct gttttataga gatagttgag ataccaggga tggtgttaaa aactacatta
      841 ttcttacgat atatcaaaaa tgattaacca tttattaatt gatgttattg taagtgtgat
      901 tcttaattat cataataaac attaagttaa ccatatccat acaaaa
//
LOCUS       DAOFQD010000346          945 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.356, whole genome shotgun sequence.
ACCESSION   DAOFQD010000346 DAOFQD010000000
VERSION     DAOFQD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.356"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(210..285)
                     /locus_tag="QBF46_004947"
     tRNA            complement(210..285)
                     /locus_tag="QBF46_004947"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Ile,seq:cat)
BASE COUNT          306 a          206 c          139 g          294 t
ORIGIN      
        1 ctgaacataa aacactatca gtaagttgga gtcattaccg agtactgatg gataaataca
       61 ttgcagtggc gtgccacgta ccaaaacacc agccctcatt cgaaaccacc caccgcactt
      121 cttccttgaa atggcgttag tcatgaaata tagaccgcca tcgagtaccc cttgtaccct
      181 taactcttcc tgatacgtaa ataatgattt ggtggccctt gctggacttg aaccagcgac
      241 caagcgatta tgagtcgcct gctctaacca ctgagctaaa gggccttgag tgtgcaataa
      301 caatacttat aaaccacgca ataaacatga tgatcatatg atgtaaataa cagattttta
      361 tgcgttccca ttcaccttgg gtcgtgatta cacgcgacat aaaacccgac accgcctcca
      421 ttcgcaaagt cgatactcgc agtcaacaag caaatgttaa taattagcac tatctatagt
      481 tatcatcgat tcaatgatac tttgtaatga ttttgtatct aataatataa ctttattaca
      541 ttagctgaag agttttcgca tcattatgat atctgttact tttcactcca taaaaataaa
      601 cttcgtatag caatatattc tttcatagat cttattctgc taatcattag tttcgtatga
      661 gcgatttttg acagttgcct ctccagacca catcgataat taataaaaca gatttaagca
      721 ttatcctttt ccatataaat attggataaa agtaggacat ctgtttgcaa ttactttcac
      781 aacaattaaa catttttatg tttccgtata catcatatta ctctaccatt agaggaactt
      841 tattatgttt tctataaaac caggacccag aaatttacct atcgacaacc ccacattgtt
      901 atcatggaac attactgacg gggatctaaa ttccaaatta aatac
//
LOCUS       DAOFQD010000347          937 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.357, whole genome shotgun sequence.
ACCESSION   DAOFQD010000347 DAOFQD010000000
VERSION     DAOFQD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.357"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..628)
                     /locus_tag="QBF46_004948"
     CDS             complement(<1..628)
                     /locus_tag="QBF46_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HDQ6677014.1"
                     /translation="MRRTFTAEEKVSVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGT"
     gene            complement(696..937)
                     /locus_tag="QBF46_004949"
                     /pseudo
     CDS             complement(696..937)
                     /locus_tag="QBF46_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          201 a          244 c          211 g          281 t
ORIGIN      
        1 tcgtacctct ttcgcctttg cgggtatgac gcctgccatg gcgaaggcta tgcgaccgtc
       61 gcagatgctg tatattcagg tggtgtagcg cttcacggct acgaaagtac agcgttttat
      121 aaattgtctc aggtgatatt cgcagcgttt tttgacgtgg ttttgttcgc cttaaccatc
      181 ctgatatttg ctctggagac catttcatct ccagcttttc cagaacaagc tttcgcaatg
      241 gtaaattttg atccagtaag cacggttttg gccttttcgc cattctgttg gctcggttat
      301 tagcatcaac agctttgtaa tagcgtctgc cccgattacg ctgaacttca cgtgagatcg
      361 tcgaaggact gcgattcagc gcagtagcta tcgcacgaat gctcattttg gctgacaaac
      421 cagctcgtat ctcctcgcgc tcagacagtg tcaggtgagc tacagcccgc ttacgctcat
      481 ggggttttat gccgccagta tcccttaaca tagtgaagat cgttccgggt tttgaaccca
      541 ggatattcgc tatttcactg aagcctgttc cgttcttcca tagttcaaaa acagagactt
      601 tttcctctgc tgtaaatgtt cgtctcattc aaaaaacctc cgcaacccca tgttttcaca
      661 taactgttgc gttgaccaat tgaatctaca gtagcctttt ttaatatcgt attttgctcc
      721 tccagacggc gaacctgctt ttccagctcg cggatacgtt gctggtctgg agtaatgggt
      781 gtggcagagg gcgtaatccc ctggcgctct ctcctgagct ggcgtaccca gctctcaagc
      841 gtagtagaac ccacattcat cgcttcactg gcctgtcgat atgagtagcc cttatcaaca
      901 atcagctgtg cacattccag cctgaactca ggggtga
//
LOCUS       DAOFQD010000348          928 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.358, whole genome shotgun sequence.
ACCESSION   DAOFQD010000348 DAOFQD010000000
VERSION     DAOFQD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.358"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            145..>928
                     /locus_tag="QBF46_004950"
     CDS             145..>928
                     /locus_tag="QBF46_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDQ6677015.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEVETTAGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSPLVEQNLMPWLATGAAALITALAVVELNDDDDHHHRNNSPL
                     PPTPPDDESDDTPVPPTPGGDEIIPDDPDDTPTPPKPISFNNDVILDKTEKTLTIRDS
                     VFTYTENADGTISLQDSNGRKATINLWQIDEANNTVALEGVSADGATKWQYNHNGELV
                     ITGDNATVNNNGKTTVDGKDSTGTEINGNNGKVIQDGDLDVSG"
BASE COUNT          281 a          206 c          213 g          228 t
ORIGIN      
        1 cctgaattat aactgtgcat tatcggaaag tatgtttgat taggtaataa tctactggca
       61 atataggatg tcttcaatgt tttaaataac taattggtcg ggttagtgca ccggctttct
      121 ttatattcgc cagaaggatt tattatgcaa aggaaaactc tattgtcggc ctgtattgca
      181 ttagctctga gtggtcaggg ttgggcggca gatatcacag aggtagaaac caccgcaggt
      241 gaaaagaaaa ataccaatgt gacttgtccg gcagacccag gaaaacttag tccggaagag
      301 cttaaacgct taccctctga atgctctcct ttagtcgaac aaaacctgat gccatggctt
      361 gccacaggcg ctgctgcgtt aatcacggcc ttagccgtag tggaactaaa cgacgatgat
      421 gatcatcatc atcgcaacaa ttctccactc ccaccgacac cacctgatga tgaatcagac
      481 gatactccag ttcccccaac tcctggcgga gatgagataa taccggacga tccagatgat
      541 acgcctacac ctcccaaacc gatttcgttt aataatgacg ttattctcga taaaacagaa
      601 aaaacattaa ctattcgcga ttcagttttt acttataccg agaatgctga cgggactatt
      661 tctctgcaag atagcaatgg tcgtaaggca acgattaatc tttggcagat tgatgaagcg
      721 aataacactg ttgcccttga aggggtgagc gcagatggcg caacgaagtg gcaatataat
      781 cacaacggtg agcttgttat tacgggtgat aatgccacag taaacaacaa tggcaaaacc
      841 accgttgacg gcaaggattc caccggcacg gaaatcaacg gcaataacgg aaaggtgatt
      901 caggacggcg atctggatgt cagcggcg
//
LOCUS       DAOFQD010000349          914 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.359, whole genome shotgun sequence.
ACCESSION   DAOFQD010000349 DAOFQD010000000
VERSION     DAOFQD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.359"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..136
                     /locus_tag="QBF46_004951"
     CDS             <1..136
                     /locus_tag="QBF46_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDQ6677016.1"
                     /translation="GGAPISVIRQYIEQQQTPGQVENRALYPRPEGWGFTAHRIKSAI
                     "
     gene            complement(154..822)
                     /locus_tag="QBF46_004952"
     CDS             complement(154..822)
                     /locus_tag="QBF46_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDQ6677017.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGQDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT          245 a          210 c          205 g          254 t
ORIGIN      
        1 cggaggtgcg ccaatatccg tcatccgcca atacattgaa caacagcaaa cacctggtca
       61 ggtggaaaac cgcgccttat atccccgccc tgaaggatgg ggttttacgg cgcaccggat
      121 aaaaagcgcc atctaacgat ggcgcgtctg gaactacttt ttgttaacgt caaacatggt
      181 ggcatcggtc gccatgtcat caataacttg tttaatattt tcgaaagcca ttggcgtact
      241 ttcgttattc aggtcttgac cttcgccctg acgaacgact ttgataaccg gtttattagt
      301 cgctgcatca atcagctcac cttcaaaata gaggcgagta tccatggtac ggtggcctgt
      361 agccatttgc gtccccgcaa ccactaatgc aacaggaacc acttcataga attgcagccc
      421 ttctttgctg gtatctacac cggtaatggc cccacggaaa atcagactac gcggcccagc
      481 ggtggtaacc agtggtttac gctgcgctat cgcttctttc atttcggtgt tggtatagtt
      541 caaaatttta tccagaactt tctgccctac ctgggtcgac ggtttcggta ccggataata
      601 agtgattggg ttccagacga tgctgtcata tttgctttga tcaaaactcg ggtctaccca
      661 acgtaaaaca ggtttacctg tagccgaggt tgtttctttt aaatcagagt aattgtttaa
      721 aaaaccagaa tatttatctg gctgggtgat tttagaggca caaccagaca gagccaataa
      781 gccgcataaa gctgcaactt tcgcaaatga tgtggtacgc atgaatagtt tcctgagatg
      841 attatattac tttttaagtt atagcaaaaa gcgccgcttc gtgttgtggc aaaaaaagtg
      901 ttggtgaaaa taaa
//
LOCUS       DAOFQD010000350          904 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.360, whole genome shotgun sequence.
ACCESSION   DAOFQD010000350 DAOFQD010000000
VERSION     DAOFQD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.360"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(137..>904)
                     /locus_tag="QBF46_004953"
     CDS             complement(137..>904)
                     /locus_tag="QBF46_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HDQ6677018.1"
                     /translation="FFPSIKEDLIAKAFQYYMNFSPEVSLLIANLSTLEGSLVQGTKL
                     STDIANLIFLEEEALLSEEVRKLGGIYTRYVDDITISFESEVNNDDISKIKLMILSMA
                     LKSGVRLNRKKSRVLRNGQSKIVHGVKVIKELRPTQKRKDNIRVCLFNLKKKVIEKES
                     VMDVLTLYFKIRGLINTLKQQGDKNHAEYIKQANQLVAGVDKKEAIKSIRKMRKVKDI
                     RKLRSLYSKLKPLGYTSKSVSAILDAEYKTCKCKINN"
BASE COUNT          286 a          175 c          102 g          341 t
ORIGIN      
        1 gaaaacaaaa aaaacaattc accacaactg agaccatcca gttatcgtgt taataaataa
       61 ttttattaca cattaatcgt aatccgtaat aaatcgatga caatccagta tgatttaccc
      121 gtaacttctc aatcagttag ttattaattt tacatttaca ggttttatat tcagcatcca
      181 aaatggcaga aacactttta gatgtatagc ctaatggttt taactttgaa tataacgatc
      241 ttaatttcct gatatcttta acctttctca ttttcctgat tgattttatt gcctcctttt
      301 tatcaacacc agctactaac tgattagctt gttttatata ctctgcatga ttcttatcac
      361 cttgctgctt taatgtattt attaatcctc taattttaaa atacaacgta aggacatcca
      421 taacactttc tttttcaatc acttttttct tcaaattaaa aagacacacc cttatattat
      481 cttttctttt ttgtgttggt ctaagctctt tgattacttt tacaccatgt acaattttag
      541 actgaccatt cctcaaaacc cttgatttct tcctatttaa tctaacccca cttttgagcg
      601 ccattgacaa tatcattaat tttatttttg aaatatcatc attattaact tcagattcga
      661 aactaatagt tatgtcatcg acatatctcg tgtaaattcc acctaatttt ctaacttcct
      721 cactgaggag agcctcttct tctaaaaata ttaagttggc aatgtcagta cttaacttag
      781 tcccttgaac aagtgagcct tcaagtgttg acagattcgc tattaataac gaaacttcag
      841 gagagaagtt catgtaatat tgaaaagctt tagctatcag atcttctttt atagaaggaa
      901 agaa
//
LOCUS       DAOFQD010000351          890 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.361, whole genome shotgun sequence.
ACCESSION   DAOFQD010000351 DAOFQD010000000
VERSION     DAOFQD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.361"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..354)
                     /locus_tag="QBF46_004954"
     CDS             complement(<1..354)
                     /locus_tag="QBF46_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HDQ6677019.1"
                     /translation="MRKFLLVAGLAALVSGCGEKGDFEKAINGKISQSKLCYSLQDND
                     IVFNKGFPIKVNRGYRSAGYSASDEILKGLAEQGLLTVSQQANGFSSVDVLEVTDKGQ
                     AVEFWDRKDGACIGHR"
     gene            complement(370..>890)
                     /locus_tag="QBF46_004955"
     CDS             complement(370..>890)
                     /locus_tag="QBF46_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677020.1"
                     /translation="TGCQQLNDGVNTVNGAISSSLSSVGKALGGTATTQSLGPVSSVS
                     GLGAVCKDYEENAMAAEKQWTGKRISITNATVLEVTKGRSASDMMTGMPKTAPNYYLL
                     FKTADTSYCGGMVWLDYYSGIDDAVLTYKKGQKISVTGTVNNFEVRGFATVNNADMAV
                     RLVVLRNGTINH"
BASE COUNT          220 a          233 c          191 g          246 t
ORIGIN      
        1 acgatgacca atacaggcac catctttacg atcccaaaac tcaaccgctt ggcctttgtc
       61 tgttacttcc agaacatcga cactgctaaa cccatttgcc tgttgtgaaa cagtgagcag
      121 cccttgttca gcaagtcctt tcaggatttc atcactggca ctgtatccgg cagaacggta
      181 gccacggtta accttaatcg gaaaaccttt gttgaataca atgtcgttgt cctgcaggga
      241 gtagcacagc tttgactgtg atattttccc gttgatcgct ttctcaaagt cgcctttttc
      301 accgcatccc gatacaagag cagcaagacc cgccacgagt aaaaatttac gcatagtgtt
      361 atcccagaat taatgattga ttgtgccgtt tctcagaaca accagacgaa ctgccatatc
      421 ggcgttgttg acggtagcga acccacggac ttcgaaatta tttacagttc cggtgacgct
      481 gattttctgg cctttcttat aggtaagaac tgcatcatca ataccagagt agtaatccag
      541 ccataccatg ccaccgcagt agcttgtatc ggctgtttta aagagcaggt aatagttagg
      601 ggctgttttg ggcattcccg tcatcatatc gctggcactt ctgcccttcg taacttccag
      661 aacagtggca ttggtgatag agatcctttt gccagtccac tgtttctctg ccgccattgc
      721 gttttcttca tagtctttgc agacagcacc aaggccagat acagacgata ccgggccaag
      781 gctttgagtg gtggcggtgc cccccagcgc tttacctact gaagacaacg aagaactgat
      841 tgctccatta actgtattca caccatcgtt gagctgctgg catcctgtca
//
LOCUS       DAOFQD010000352          886 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.362, whole genome shotgun sequence.
ACCESSION   DAOFQD010000352 DAOFQD010000000
VERSION     DAOFQD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.362"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..419)
                     /locus_tag="QBF46_004956"
     CDS             complement(<1..419)
                     /locus_tag="QBF46_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HDQ6677021.1"
                     /translation="MAMKMKIKKGFSLLELTLVLGVGTMVAFMKFQDMKNEQESIMAS
                     AVGQQMKQIGEAVNGYINIRYDKLSTLSNAAGTGTDPGPRTCSGSVCEITYQTLINEG
                     LLPSTYTGTNANKSSYKIILKRDGTSPNYVINGLITTS"
     gene            complement(570..>886)
                     /locus_tag="QBF46_004957"
     CDS             complement(570..>886)
                     /locus_tag="QBF46_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HDQ6677022.1"
                     /translation="FPDNMLSGSGSSATPINAFKGNVVVESADTGPSAAAGSSFTITY
                     KNVPAAECTKIITAAAGNFYTAGVGTVGNVKPAGGVLDVAGTATQCQNGGNDNTLIFT
                     SI"
BASE COUNT          286 a          171 c          149 g          280 t
ORIGIN      
        1 gatgtcgtta taagaccatt aataacatag ttaggtgaag ttccatcacg ttttaatatg
       61 attttatacg aggatttatt agcattagtg cctgtgtagg ttgaaggtag taaaccttcg
      121 ttaatcaatg tctggtaagt aatttcacat actgaacctg aacaggttct tggacccgga
      181 tcggttcctg ttccggcagc attggataag gttgataatt tatcataacg gatatttata
      241 tagccattaa ctgcttcacc gatctgtttc atttgttgac cgacagcaga agccattata
      301 ctctcctgtt catttttcat atcctgaaac ttcataaaag ccaccatcgt cccaacacct
      361 aaaacaagag ttaactctaa taaagagaat ccttttttaa ttttcatttt catcgccatt
      421 tttatattca cgtattaatt aattatcaaa taacaaacaa aaatcaagat ggaagaagaa
      481 ctatatatag aagaataaat accaatagat gaataaaaag gcccctttaa ataaggagcc
      541 aaaaaaaata atttattaaa acattgatgt taaatagaag tgaagatcaa tgtattatca
      601 tttccaccat tttgacactg tgtagccgtt cctgcgacat ccagcacacc tccagcaggt
      661 ttaacattac cgaccgtgcc tactcctgcc gtatagaaat tgcctgccgc tgctgtaata
      721 atttttgtac attctgctgc agggacattc ttatatgtta tggtgaatga ggaacctgca
      781 gccgcagaag gtcctgtatc tgctgattca accacaacat ttcctttgaa tgcatttatt
      841 ggtgttgctg aagaaccaga accacttaac atgttatctg ggaata
//
LOCUS       DAOFQD010000353          874 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.363, whole genome shotgun sequence.
ACCESSION   DAOFQD010000353 DAOFQD010000000
VERSION     DAOFQD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.363"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            97..294
                     /locus_tag="QBF46_004958"
     CDS             97..294
                     /locus_tag="QBF46_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HDQ6677023.1"
                     /translation="MLIVNNKILNLPIRKLFPTPIASSYGMNFEFMPELKWSFGYPGA
                     IIFMILAGLAPYLYFKRKNWL"
     gene            complement(335..715)
                     /locus_tag="QBF46_004959"
     CDS             complement(335..715)
                     /locus_tag="QBF46_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HDQ6677024.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
BASE COUNT          289 a          168 c          154 g          263 t
ORIGIN      
        1 tgagaacaaa tgctagggta aaaaaatatt taaaaaagaa gtacacttca cattttttca
       61 ttacatctct gaagagaacg tcgttaatga ttggtcttgc tcattgttaa taacaaaata
      121 ttgaatttac cgattaggaa actttttcct acgccgattg cctccagcta tggcatgaac
      181 tttgagttta tgccagaact gaagtggagt ttcggctacc caggcgcgat tatctttatg
      241 atcctcgcgg gcctggcacc gtatctgtac tttaagcgca agaactggct gtaaaaagaa
      301 ggcggtggct tagcttggct aagccacctg ttattcaaag gctccaggta tttaaccctt
      361 ttacctcttt ctcataaaac catttgttcg tgttaacagc aacataggct gctacggcaa
      421 tgcccagaat gttaacgcca attagtgcac caacaaatag agatagaata ccaagtaata
      481 gaactataat tgcttttttc cataacccaa ggacaaataa atatatccag gagcagaaga
      541 aagcaatgaa attcatttga atagttaagc gttgtcttac ttttaatgct ttaaatgctg
      601 ctttatattc tggtgttgcc ccccagaatc caggaaaacc atgttgatca taaaaattaa
      661 atcggtattt ccatttttca cttaatgaac catcgttcat atattcctta ctcataaata
      721 ctccataact attgttttga tgaatcagta ggtgcaagca ttagcatact gaaagtggaa
      781 aaataacaaa tcaaaaaaat catcgaacca ttgcctgaac aggcaaaatc ttcggctatc
      841 attgtgatga tagagatgat atatactgct aatg
//
LOCUS       DAOFQD010000354          864 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.364, whole genome shotgun sequence.
ACCESSION   DAOFQD010000354 DAOFQD010000000
VERSION     DAOFQD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.364"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            56..>864
                     /locus_tag="QBF46_004960"
     CDS             56..>864
                     /locus_tag="QBF46_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HDQ6677025.1"
                     /translation="MEFESIFTAAQFELNNRIKNPTKKSSVYDSRSSDGASMDLVTKA
                     IQESVRGTASMEGLVIGDDLLELSGEGVARKNIRRIFGKVGEPSMSIGCHPDFLHLTN
                     SSELENGYAITLFMDISGSTKLGKKYPIETVFNIKNTIIKYAIEIIQAFDGHVHRIMG
                     DAVMAFFRTKEHEASESLMMKNAVDSINCATYLIEFMEKVITPIIEEQGADEPVGIRV
                     GVDYGNNDDVIWGNYGAFGAFEVTATSYNVDVAAKLQHAAKTNTIMIGYNL"
BASE COUNT          299 a          119 c          191 g          255 t
ORIGIN      
        1 ttaattttaa tgctaatatc atccctgatt ttaataattg catagaaggt tgagaatgga
       61 atttgaaagt atttttactg cggctcagtt tgagttaaat aataggataa aaaatccgac
      121 aaagaaatca tccgtatatg actcacgctc aagtgatggt gcatccatgg atttggtaac
      181 aaaagcaatc caggaatcgg ttagaggcac cgcatcaatg gaaggccttg taataggaga
      241 tgatttactt gaactttctg gtgaaggggt tgcaaggaaa aatattagga gaatatttgg
      301 taaggtgggg gaaccaagta tgtccattgg atgtcacccg gattttctcc atcttactaa
      361 tagttcagaa cttgaaaatg gatacgctat tactttgttt atggatattt ctggctcaac
      421 taaacttggc aaaaaatatc ccatagaaac agtttttaat ataaagaaca caataataaa
      481 atacgccatt gagattatac aagcctttga tggacatgtt cacagaataa tgggagatgc
      541 tgtaatggca tttttcagaa caaaagagca tgaggcgagt gaaagtctta tgatgaaaaa
      601 tgcagtcgat tcgataaatt gtgctacgta tttaatagaa ttcatggaga aagtaataac
      661 accgataatt gaggagcagg gtgctgatga gcctgtaggt attagagttg gagtagatta
      721 tggtaataac gatgatgtaa tctggggaaa ctatggtgct tttggtgctt tcgaggttac
      781 agcgacttca tataatgttg atgtagctgc aaaattacaa catgcagcta aaacaaacac
      841 tataatgatc gggtataatt taaa
//
LOCUS       DAOFQD010000355          844 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.365, whole genome shotgun sequence.
ACCESSION   DAOFQD010000355 DAOFQD010000000
VERSION     DAOFQD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.365"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>844
                     /locus_tag="QBF46_004961"
     CDS             <1..>844
                     /locus_tag="QBF46_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HDQ6677026.1"
                     /translation="FKTFNPSTSLKIPLASVCFYYKDKGFNLIDCLDKIYWEFTREGS
                     LSKIDSDSGIITSVCFDSSRFLKDTLFFQPSLEFVIIREVGDIVAIYDSRGRKYNKRI
                     ELGIRQPVGPDKLFKLISLNEKSKTTQASTRAIQINSQQAESILYVSDRLESTTSTQA
                     NGSYAVSTTIGSNLHDDLSIMSSYYLGVGSGRVSDQKERQFSFERFCEWVNDVKTNLE
                     GANKVSSSFLNSFAQTVDGIPTEKPVACIIDLSNYNGLLKVYCNGKHKKITSNYLFKK
                     YNFGI"
BASE COUNT          291 a          130 c          148 g          275 t
ORIGIN      
        1 ttcaaaacat tcaacccttc cacatcctta aaaatacctc tcgcgtcagt atgtttttat
       61 tacaaagata agggctttaa tctaattgat tgtttagata aaatatattg ggaatttaca
      121 agagaagggt ctttatcaaa aattgatagt gactctggca tcattacatc agtttgcttt
      181 gacagttcca gatttcttaa ggatacgtta ttctttcaac catcacttga gtttgtaatt
      241 atacgtgaag taggtgatat tgttgcgatt tatgatagtc gcggtagaaa atacaataaa
      301 agaattgaat taggtatcag acaaccagtt ggcccggata agctatttaa gctaatttcg
      361 ctaaatgaaa aatctaaaac aacgcaagct agcacaaggg ctatacagat taattcacag
      421 caagcagaaa gtattcttta tgtgtctgat aggttagagt caacgacttc aactcaagcc
      481 aatggaagct atgcagtatc aactacaata ggttctaatc ttcatgatga tctttcgatt
      541 atgtcctcat attatttagg cgtaggatcc ggccgtgtta gtgatcaaaa agaaagacaa
      601 tttagttttg aaaggttttg tgaatgggtt aatgatgtaa aaaccaattt agaaggcgct
      661 aacaaagtat ctagttcatt tcttaattca tttgctcaaa ctgttgatgg tatacctact
      721 gaaaaaccag ttgcttgtat tattgatcta agcaactata acggtttgtt gaaagtgtat
      781 tgtaatggca agcacaaaaa aataacatca aactatctat ttaaaaaata taattttggc
      841 attt
//
LOCUS       DAOFQD010000356          833 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.366, whole genome shotgun sequence.
ACCESSION   DAOFQD010000356 DAOFQD010000000
VERSION     DAOFQD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.366"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>833
                     /locus_tag="QBF46_004962"
     CDS             <1..>833
                     /locus_tag="QBF46_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HDQ6677027.1"
                     /translation="LLRAGRFDRQVLVDRPDKKGRLDILKVHVKKVTLAQDVDLEQVA
                     ALTTGFSGADLANRVNEAALAARRRRASAVELQDFTATIERIVAGLEKKSRVLNPKER
                     ETVAHHEMGHALVALALPETDPVHKISIIPRGIGALGYTLQRPTEDRFLMTRTDLEHK
                     IAVLLGGRAAEKLVFGELSTGAADDLARATDIARDMITRFGMDEGLGYIAFEAQRPRF
                     LDTPELAHGGCRVAESTQARIDQAIRDIVMGVFERAYRILDINRAVLERCARELLARE
                     TL"
BASE COUNT          150 a          271 c          285 g          127 t
ORIGIN      
        1 ctgctgcgtg ccggccgctt cgaccgccag gtgctggtgg accggcccga caagaagggg
       61 cggctggaca tcctgaaggt tcatgtcaag aaggtgacgc tggcccagga tgtagacctc
      121 gaacaggtag cagcactgac cacgggcttt tccggcgcgg acctcgccaa tagggtcaac
      181 gaggcggcgc tggcggcgag gagacgccgt gcgtccgccg tggaattgca ggacttcacc
      241 gcgaccatcg agcgcatcgt ggcgggcctg gaaaagaaga gccgcgtgct caatcccaag
      301 gagcgggaaa ccgtggccca tcacgagatg ggccatgcgc tggtggcgct ggcgctgccc
      361 gaaaccgacc ccgtacacaa gatctcgatc atcccgcgcg gcatcggcgc gctgggctac
      421 accttgcagc gccccaccga agaccgcttc ctgatgacgc gtaccgatct cgagcacaag
      481 atcgccgtac tgctgggcgg gcgtgccgcc gaaaagctgg tgttcggcga gttgtctacc
      541 ggggcggcgg acgatctggc gcgagccacc gacatcgccc gcgacatgat cacccgcttt
      601 ggcatggacg agggcctggg ctacatcgcc ttcgaggcgc agcggccccg ctttctcgat
      661 acaccggaac tggcccacgg cggttgccgg gtggccgaat cgacccaggc gcgcatcgat
      721 caggctatcc gcgacatcgt gatgggcgtg ttcgagcgcg cctaccggat tctcgacatc
      781 aaccgcgcgg tgctggagcg ctgcgcgcgc gagctgctgg cgcgggaaac gct
//
LOCUS       DAOFQD010000357          827 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.367, whole genome shotgun sequence.
ACCESSION   DAOFQD010000357 DAOFQD010000000
VERSION     DAOFQD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.367"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(48..>827)
                     /gene="corA"
                     /locus_tag="QBF46_004963"
     CDS             complement(48..>827)
                     /gene="corA"
                     /locus_tag="QBF46_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDQ6677028.1"
                     /translation="DIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRL
                     FTLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLS
                     RVIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQAR
                     EILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASS
                     YGMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT          212 a          227 c          200 g          188 t
ORIGIN      
        1 ttttagaaat aagccggata aaaaaatagc tttatccggc actcatatta caaccagttc
       61 ttccgcttaa agtacagata cggtgccagg cccgcgagga tcataaagat aatcgcgcct
      121 gggtagccga aactccactt caactctggc ataaattcaa agttcatccc gtagctggaa
      181 gccaccagcg tcggtggcag gaacaccacg gataccaccg agaagatttt gatgatgcgg
      241 ttctgctcga tgttgataaa gcccatcgcc gcctgcatca ggaagttcac cttctggaac
      301 agggattcgt tatgcggcag cagggattcg atatctcgca ggatttcacg cgcctgctcc
      361 agttgcccac ccggtaaacg cgccttgcgt accaggaagt tgagcgcacg ctgggtatcc
      421 atcagacaca ggcgaacttt ccagccgata tcttccagtt ccgccagcgt ggagagcgcc
      481 tcatcgtact catcgccctg atgcccttcc ataattaccc ggctcaactg ctccagatcg
      541 ctgtagatat tttcaatttc atctgccagc tgttcgattt tggtttcgaa cagatccaac
      601 agcagctcgt aggcgttacc gtccaccatt gactggctac gggcgcgcat acgatacaga
      661 cggaatgcag gcagttcacg ctcacgcaga gtaaacagac gaccatcacg gatggtaaat
      721 gccacagtgg agttaccggc gtgatcttcc gcatcttcaa agaagaagaa ggagtgaata
      781 tgcaggccgt cgtcgtcttc aaagaaacgt gccgatgctt cgatgtc
//
LOCUS       DAOFQD010000358          805 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.368, whole genome shotgun sequence.
ACCESSION   DAOFQD010000358 DAOFQD010000000
VERSION     DAOFQD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.368"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            38..370
                     /locus_tag="QBF46_004964"
     CDS             38..370
                     /locus_tag="QBF46_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020806197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677029.1"
                     /translation="MNNIKIITLFHTNKKIPFMTCIVKDVEENEQGIKLTLESGDNIH
                     VKDYGYFFLSESANEFDQERMINTYRRLISEPSQVSEETIESLVPKISNNMKTNFPAS
                     GVSALEDD"
BASE COUNT          295 a          123 c          135 g          252 t
ORIGIN      
        1 atggtattaa ataataaaaa aagaaggaaa ataaaggatg aacaacatta agattattac
       61 gctctttcat acaaataaaa aaataccatt tatgacttgt attgttaaag atgttgaaga
      121 aaatgaacaa ggaataaaac tcactttgga aagtggtgat aatatccatg tcaaagatta
      181 tggctatttc ttcttatcag aatcagcaaa tgagtttgat caggaacgaa tgattaatac
      241 atacagaaga ttgatttctg aacccagcca agttagcgaa gaaacaatag agtccttagt
      301 tccgaagata tcgaataata tgaaaaccaa tttcccggca agtggtgtga gtgctttgga
      361 agatgattga aaagtctgaa tggtagctac cattccatat gaaacagtat attaaaacat
      421 gcgttgcata caggttttta caacaccata tctgaagata gctacattag ctgagatcta
      481 acaatacctg tctgcgtaat ggtcgacagg gtattatctg ttccgaccta tacctttcta
      541 tctccatcca tgtgcaaaca gctactgaca tggctcttcg tgtacattac acctcttatt
      601 gatcgatata aaagatcaat aaactgcatt tgatagattt taactatcaa attagcgtca
      661 attgatcggt gttttaattg tttatatcga tcgattatag atatatgata gttttaatct
      721 atcatatgat ttgaattcga tcgatacaaa cgatatgacg aacagaaaaa taaattgaat
      781 cctatcaatt gattaatgct ttttt
//
LOCUS       DAOFQD010000359          780 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.369, whole genome shotgun sequence.
ACCESSION   DAOFQD010000359 DAOFQD010000000
VERSION     DAOFQD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.369"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>780
                     /gene="proV"
                     /locus_tag="QBF46_004965"
     CDS             <1..>780
                     /gene="proV"
                     /locus_tag="QBF46_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDQ6677030.1"
                     /translation="QILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGKSTMVRLLNR
                     LIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLDNTAFGMELA
                     GINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINPDILLMDEAF
                     SALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGNRIAIMQNGEVVQVGTPD
                     EILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKT"
BASE COUNT          206 a          182 c          200 g          192 t
ORIGIN      
        1 aacaaattct ggaaaaaact gggctatcgc ttggcgtaaa agacgccagt ctggccattg
       61 aagaaggcga gatatttgtc atcatgggat tatccggctc gggtaaatcc acaatggtac
      121 gccttctcaa tcgcctgatt gaacccaccc gcgggcaagt gctgattgat ggtgtggata
      181 ttgccaaaat atccgacgcc gaactccgtg aggtgcgcag aaaaaagatt gcgatggtct
      241 tccagtcctt tgccttaatg ccgcatatga ccgtgctgga caatactgcg ttcggtatgg
      301 aattggccgg aattaatgcc gaagaacgcc gggaaaaagc ccttgatgca ctgcgtcagg
      361 tcgggctgga aaattatgcc cacagctacc cggatgaact ctctggcggg atgcgtcaac
      421 gtgtgggatt agcccgtgca ttagcgatta atccggatat tttattaatg gatgaagcct
      481 tctcggcgct cgatccatta attcgcaccg agatgcagga tgagctggta aaattacagg
      541 cgaaacatca gcgcaccatt gtctttattt cccacgatct tgatgaagcc atgcgtattg
      601 gcaaccgaat tgccattatg caaaatggtg aagtggtaca ggtcggcaca ccggatgaaa
      661 ttctcaataa tccggcgaat gattatgtcc gtaccttctt ccgtggcgtc gatattagtc
      721 aggtattcag tgcgaaagat attgctcgcc ggacaccgaa cggcttaatt cgtaaaaccc
//
LOCUS       DAOFQD010000360          773 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.370, whole genome shotgun sequence.
ACCESSION   DAOFQD010000360 DAOFQD010000000
VERSION     DAOFQD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.370"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..>773)
                     /locus_tag="QBF46_004966"
     CDS             complement(<1..>773)
                     /locus_tag="QBF46_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDQ6677031.1"
                     /translation="LNINAMVAGAKYRGEFEERVQKVLKEVTEHQGELILFIDEVHTI
                     VGAGQGGGEGGLDVANVFKPMMARGELNLIGATTLNEYQKYIEKDAALERRFQPVTVP
                     EPTVAQTIMILRGLRDTFEAHHKVSISEDAIIAAAELSDRYITARFLPDKAIDLLDQA
                     AARVKLSATARPVAVQELESELHQLRREQDYVAARKQYDQAAELGKRIEAKEAELKKL
                     VENWERERASGSAEVKAEHVAQIVSRLTGIPVNELTVEE"
BASE COUNT          104 a          272 c          237 g          160 t
ORIGIN      
        1 gctcttccac cgtcaactcg ttgaccggga tgccggtcag gcgcgagacg atctgcgcca
       61 cgtgttccgc cttgacctct gcactgccgg aggcccgctc ccgctcccag ttctcgacga
      121 gcttcttgag ctcggcctcc ttggcttcga tgcgcttgcc gagctcggcg gcctggtcgt
      181 actgcttgcg cgcggccacg taatcctgtt cacgccgcag ctggtgcagt tcggactcca
      241 gctcctgcac ggccaccggc cgggccgtgg ccgacagctt cacgcgcgcg gccgcctggt
      301 cgagcaggtc gatcgccttg tccggcagga agcgcgccgt gatgtagcgg tcggacaact
      361 cggccgccgc gatgatcgcg tcctcggaga tgctgacctt gtggtgcgcc tcgaaggtgt
      421 cgcgcaggcc gcgcagaatc atgatggtct gggccaccgt cggctcgggc accgtcaccg
      481 gctggaagcg ccgctccagt gcggcatcct tctcgatgta tttctggtac tcgttcagcg
      541 tcgtggcgcc gatcaggttg agttcaccgc gcgccatcat cggtttgaat acgttggcca
      601 cgtccagccc gccttcgcca ccgccctggc cggcaccgac gatggtgtgc acctcgtcga
      661 tgaacaaaat cagctcgccc tggtgctcgg tcacctcctt cagcaccttc tgcacgcgct
      721 cctcgaactc gccgcgatat ttggcgccgg ccaccatggc gttgatgttg agt
//
LOCUS       DAOFQD010000361          765 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.371, whole genome shotgun sequence.
ACCESSION   DAOFQD010000361 DAOFQD010000000
VERSION     DAOFQD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.371"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>765
                     /gene="wzy"
                     /locus_tag="QBF46_004967"
     CDS             <1..>765
                     /gene="wzy"
                     /locus_tag="QBF46_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158642102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O25 family O-antigen polymerase"
                     /protein_id="HDQ6677032.1"
                     /translation="CLLSMLFNIEQVLSFKIVLSMYSILIMAYVSSCYAQTLWLCSEE
                     ILKRSVFYLFAFLCLIGIISILLQKTEIIHDKSMILFPEPSAFALVFIPIFSFCLYYT
                     RGGGVLLLYILSLGIALGIQNLTMLVGIVISVFVMKKITIRQTIVIFLGAWIFSMILS
                     DLDISYYTSRLDFKNTTNLSVLVYLSGIERAFLNFITSYGLGIGFQQMGVNGEVGVYQ
                     QILADLDAPMLNIYDGSFISSKLISEFGFIGAIMCIF"
BASE COUNT          211 a          101 c          145 g          308 t
ORIGIN      
        1 tgtctgttaa gtatgctatt taatattgaa caggttttat catttaaaat tgtactttca
       61 atgtatagca tcttaataat ggcatacgtc tcctcttgtt atgcacagac tttgtggtta
      121 tgttctgaag aaatacttaa gagatccgtc ttttatttgt tcgcatttct ttgccttatt
      181 ggcattataa gtattctttt acagaaaact gagattatac atgataaaag tatgattctt
      241 tttcctgaac catcagcatt tgcattggtt ttcataccta tcttttcatt ttgtttatac
      301 tatacaaggg gggggggggt actattgctc tatatattat ctttgggtat tgcgttaggt
      361 atccagaact taacgatgtt agtaggcatt gtgattagtg tttttgtgat gaaaaaaata
      421 acaataaggc aaactattgt tatattttta ggggcatgga ttttttccat gatattaagt
      481 gatttagaca tttcttacta tacatcgcgg cttgatttta aaaatactac gaacttatca
      541 gtgcttgtat atctttcagg aattgaaaga gctttcttga attttattac aagttatggt
      601 cttggtattg gttttcaaca aatgggcgtg aatggggagg taggagtgta tcaacaaatt
      661 ttagctgatc ttgatgcccc tatgttaaat atatacgatg gctcatttat ttcttcgaag
      721 ttaatatctg agtttgggtt tattggtgca ataatgtgca ttttc
//
LOCUS       DAOFQD010000362          756 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.372, whole genome shotgun sequence.
ACCESSION   DAOFQD010000362 DAOFQD010000000
VERSION     DAOFQD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.372"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            63..>756
                     /locus_tag="QBF46_004968"
     CDS             63..>756
                     /locus_tag="QBF46_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ6677033.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKV"
BASE COUNT          250 a          131 c          167 g          208 t
ORIGIN      
        1 cgaattaacg catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc
       61 cagtgggacg taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt
      121 ctaaaattta tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg
      181 atccagaatt aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc
      241 caaagcacgt tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg
      301 tgcagggacg ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga
      361 cttcaaatgt taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca
      421 atatcggagc ggcaggttct gtcagctata tgtttgacaa tacgggtgtg attgtattta
      481 aaggatcaga ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg
      541 atgtgactga agaagaaggt aacattgtta tttataccga acctactgac cttcataaag
      601 gaatcgcggc tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga
      661 ttgctcaatc tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg
      721 atgcccttga agatgacgac gatgtacaaa aagttt
//
LOCUS       DAOFQD010000363          753 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.373, whole genome shotgun sequence.
ACCESSION   DAOFQD010000363 DAOFQD010000000
VERSION     DAOFQD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.373"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..377)
                     /locus_tag="QBF46_004969"
     CDS             complement(<1..377)
                     /locus_tag="QBF46_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDQ6677034.1"
                     /translation="MQGLLGTTLILLAACSLAAMATAAFRVPALLGYLSVGVVLGPSV
                     IGVIAPGETLSFLSELGVALLLFMVGLEFSLGDFWLARRTVLMAGALQMIAVAPPLIL
                     LLMWLGQPGQSAALLGTAAAMSST"
     gene            complement(381..716)
                     /gene="trxA"
                     /locus_tag="QBF46_004970"
     CDS             complement(381..716)
                     /gene="trxA"
                     /locus_tag="QBF46_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDQ6677035.1"
                     /translation="MFTGEPIELTTATFSRHVERSDLPLLVDFWAPWCGPCKMMAPQF
                     QQAAHQLEPTIRLAKVNTEAEPHLAAQFGIRSIPTLALFQGGREIARQAGVMGAQDIV
                     RWTSTQVGR"
BASE COUNT          141 a          265 c          242 g          105 t
ORIGIN      
        1 gtggacgaca tggccgccgc agtgccgagc agcgcggcgc tctggccggg ctgccccagc
       61 cacatcaata gcaggatcag cggcggggcc acggcgatca tctgcagagc gcccgccatc
      121 agtaccgtcc tgcgggcgag ccagaagtct ccgagggaga attccagtcc gaccatgaac
      181 aacagcagcg ccacgcccag ctcggacaga aagctcagcg tctcccccgg cgcgatcacg
      241 ccgatgactg aggggcccag tacaacgccg acagagagat accccagcaa ggcgggcact
      301 ctgaacgccg ccgtcgccat cgccgccagg ctgcaagccg ccagcaggat tagcgtagtg
      361 ccgagcaagc cctgcatcgg tcagcgtccc acttgcgtcg acgtccagcg cacgatgtcc
      421 tgcgcgccca tcacgccggc ctgacgggcg atctcccgcc caccctggaa cagggcgagc
      481 gtcgggatgc tgcggatacc gaactgcgcg gccaggtggg gctcagcctc ggtattcacc
      541 ttcgccagcc ggatcgtcgg ttcgagctgg tgcgccgctt gctggaactg tggcgccatc
      601 atcttgcacg gcccgcacca tggcgcccag aaatcgacca gcagcggcag atcgctgcgc
      661 tccacgtggc gcgagaacgt cgccgtggtc aactcgatgg gctcgcccgt gaacaagggc
      721 tgctggcagc ggccacagtt gggatgttcc gat
//
LOCUS       DAOFQD010000364          752 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.374, whole genome shotgun sequence.
ACCESSION   DAOFQD010000364 DAOFQD010000000
VERSION     DAOFQD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.374"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            1..752
                     /gene="espX1"
                     /locus_tag="QBF46_004971"
                     /pseudo
     CDS             1..752
                     /gene="espX1"
                     /locus_tag="QBF46_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          287 a          136 c          102 g          227 t
ORIGIN      
        1 agctataact tgcctaaacc atctccagtc cttcatcatt ttttttcaat atactcatct
       61 gagagatgca tatttttata gatgtaaact catagaacat acagttcaat tttatttttt
      121 taaacacaaa ggcatcacat tacttagatt ggatgttttt gatgacagaa caagcgaatg
      181 tctttctgaa gagattaaga tatatcaaga atgccatgaa aaattcgtta agtttttaaa
      241 ggcaaacttc aaccatgaaa tatacccaca gctatatact cctgaaatat tttatgaggc
      301 ctgcagaaat ctccagtcat tttatgctca tcaggagaca tcacaaaacg ctaaatatag
      361 tgcaccagac aataaaaaaa gtcatcttaa ccaggaaatc aaaaagctga ttaaaaaaaa
      421 tattttccct gagctataca atgagcaatg caatgagcaa tgcaataaaa tcccctcctc
      481 atcgatagat gataaccagg gaataatgtg gcagaacttt aaaactagca atattgcata
      541 ttcacaacta tgtaaaaaat tatctttatt taaaagcata cccagtcgat taatagaaaa
      601 atcagcttat tgctcaactg aaaacacaat caccaataaa tttgatgttg tcttttctta
      661 ctgcggagat aatgtaaagg aatttatact tttattaccc tataataaat ctctggagat
      721 gcatgaactt aacgagcaaa acatccagta tt
//
LOCUS       DAOFQD010000365          724 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.375, whole genome shotgun sequence.
ACCESSION   DAOFQD010000365 DAOFQD010000000
VERSION     DAOFQD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.375"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..435
                     /locus_tag="QBF46_004972"
     CDS             <1..435
                     /locus_tag="QBF46_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ6677036.1"
                     /translation="KLRGQQKLLDGIDIIEIIYIKRPSKDLANLRKEFNKTVRKNFLI
                     KLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGTNDFENLVLIENEPYHK
                     VFTNMQSRIAKGILVGESKITPWAIPSGSIYPPMKNIMDHTK"
     gene            432..>724
                     /locus_tag="QBF46_004973"
     CDS             432..>724
                     /locus_tag="QBF46_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677037.1"
                     /translation="MMTKTQINKLIKMMNDLDYPFEAPLKESFIESIIQIEFNSNSTN
                     CLEKLCNEVSILFKNQPDYLTFLRAMDGFEVNGLRLFSLSIPEPSVKNLFAVN"
BASE COUNT          281 a          118 c          109 g          216 t
ORIGIN      
        1 aaacttagag gtcaacaaaa acttcttgat ggtatagaca taatagaaat aatatacatt
       61 aagagacctt caaaagactt agctaactta cgaaaggagt ttaataaaac tgtaagaaaa
      121 aattttctta tcaaacttgc aaaaacctcc gaagcatctg gaagattcaa cgccgaagac
      181 cttttaagaa tgagaaaggg caatgttcct ctaaattata atgttcacca taaactatct
      241 ctagatgatg gtggtactaa tgatttcgaa aatttagtat taatcgaaaa cgaaccatat
      301 cataaagttt ttactaacat gcaatcacga atagctaagg gaatattagt aggtgaaagc
      361 aaaatcactc cctgggccat tccatctggc tcaatttatc ctcccatgaa aaatattatg
      421 gaccacacaa aatgatgact aaaacccaaa taaataaatt aataaaaatg atgaatgatt
      481 tagactatcc atttgaagca ccgctcaagg aatcatttat tgaaagtata atccaaatag
      541 aatttaattc taattcaact aattgcctgg agaagttatg taatgaagtt agtattcttt
      601 ttaagaatca acctgattat cttacttttt taagagcaat ggatggattc gaagttaatg
      661 gattacgatt atttagcctc tcgattccag aaccttcagt taaaaacctt tttgccgtaa
      721 atga
//
LOCUS       DAOFQD010000366          713 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.376, whole genome shotgun sequence.
ACCESSION   DAOFQD010000366 DAOFQD010000000
VERSION     DAOFQD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.376"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          242 a          129 c          101 g          241 t
ORIGIN      
        1 aaaacatttc tcgttttact aaagaaatta cactgctgtt tttataagcc ttagatgtgc
       61 gcttcgagtt gatgttacgc agactccccg ctttggcatc gacaataacc ttgagaatga
      121 atcaaacact ttcgctccta ccttctcaga cctgtacaat caggtttaac gttattgctt
      181 tcgagaacta aaatgttttt ggaaatggta tttgaaaagc ttgtaaaaag ttgagattta
      241 gatttgacaa atatgaaaag attatttaat actttattat atttgtaatc acgttccgtt
      301 taactgatta caaggcttca aaagtcggca aaactttttt agtccttaaa aagtcaactt
      361 tgaaagttgg ctttttttgt atctttattt tatttacaac accgcattta gattgtcaaa
      421 taaaactcaa aaaaatattt taatagcaca tgatctatta tcacctataa gcagcacttg
      481 gtgattgcca caaacaataa catcgccgga atagggcttc gcactgttaa tgatggattt
      541 ataaaaaagg tgtttataag agtctacaca aaaatgtaac accacctgca agtaattaca
      601 caacacatag gtatgcaaac actaacctta aacgtctaat actcatcgcg ttacttattt
      661 tctctcattc atgattttaa attcacttct taatttctga caagaaatac aca
//
LOCUS       DAOFQD010000367          704 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.377, whole genome shotgun sequence.
ACCESSION   DAOFQD010000367 DAOFQD010000000
VERSION     DAOFQD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.377"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            39..704
                     /locus_tag="QBF46_004974"
                     /pseudo
     CDS             39..704
                     /locus_tag="QBF46_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611389.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          185 a          163 c          199 g          157 t
ORIGIN      
        1 gacaaatctg ccctatagtt tgagcatagg aggagtctat gggcacacca cgttttaccc
       61 ctgaatttaa ggaagaggcc gtccgccaga tcaccgagcg gggctattct attgctgaag
      121 tatctgaacg gctcggcgtg tcggcgcaca gcctatataa atggctccgg gctgttaagc
      181 ctgacaacag tggacagcag gcacaggatt tactggatgc cagaacagaa attctcaggc
      241 tgaaagcaca gcttaaacgc actgaggaag agcgggatat tctgaaaaag gcagcgcggt
      301 actttgcaag ggagcccgac tgaagtatcg ctttatcaac gatcaccgtg aaatctggtc
      361 gatcgttaca atgtgtcggg tccttaaggt tgcccgtgcc ggattttatg tctggctcca
      421 caaccctgta tctgccggag aaaaggataa ccagcgactg ctggaactca tccgcgactc
      481 ctatacgctg agtggtggag tttacggtta tcgcagggtt catggcgatc ttcgcgaaat
      541 tggcgaagtg tgcagcagaa accgggttgc caaaatcatg aggaaaaaca ggattcaggc
      601 tatacatggt tataaagtgc cacgcgggag tcggggacga ccgtcactga tagcgcctaa
      661 tcacgtgcgg cgtgaattta ctgtagtgaa gcccaatcag gtgt
//
LOCUS       DAOFQD010000368          688 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.378, whole genome shotgun sequence.
ACCESSION   DAOFQD010000368 DAOFQD010000000
VERSION     DAOFQD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.378"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            162..359
                     /locus_tag="QBF46_004975"
     CDS             162..359
                     /locus_tag="QBF46_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677038.1"
                     /translation="MDIINLTLQEWFESPEEIKIKRISVPEVKTILSSYHEFLIKCFS
                     GDDYVLYYKRIEISANKQTVI"
     gene            376..663
                     /locus_tag="QBF46_004976"
     CDS             376..663
                     /locus_tag="QBF46_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004860207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677039.1"
                     /translation="MSEKLMKESQKVFMHMAGLFYEMKMNTLKEVRPDEAEMLMEDDA
                     FMDSIYKDCIKNASASFKKVVRWEYFEQGHSVKMVDKEVVLITLRVNHNRR"
BASE COUNT          261 a           76 c          144 g          207 t
ORIGIN      
        1 aaatacccgt taaaatatcc gcaagtaatg ctgagacagc aaactttgct actgatgttg
       61 taaatgtcta caatgagttt atacattact tatattacaa tctaagggag gatgcttttc
      121 tgaaaaaaat gaaggatgga ttttacaggg agatattcat aatggatatt attaatctca
      181 cgttacagga atggtttgaa agtcctgaag agataaagat taaacgcata tcagtcccgg
      241 aggttaaaac tatactaagt agctatcatg agtttttgat taaatgtttt agtggggatg
      301 attatgttct ttattacaaa agaatagaaa tcagcgcaaa taaacagact gtaatataaa
      361 ataagaggta atgaaatgag tgaaaaactg atgaaagaat ctcaaaaagt atttatgcat
      421 atggcaggtt tgttttatga aatgaaaatg aatacgctta aggaagtccg acccgatgaa
      481 gcagagatgt taatggagga tgatgcattc atggatagta tttataagga ttgtatcaaa
      541 aatgcaagcg catcttttaa aaaggtagtc agatgggagt actttgaaca agggcattca
      601 gtaaaaatgg ttgataagga ggtggtgttg ataacattac gtgttaatca caatcgaaga
      661 taaaaaggtt atttaataaa tcattttt
//
LOCUS       DAOFQD010000369          685 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.379, whole genome shotgun sequence.
ACCESSION   DAOFQD010000369 DAOFQD010000000
VERSION     DAOFQD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.379"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..165
                     /locus_tag="QBF46_004977"
     CDS             <1..165
                     /locus_tag="QBF46_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HDQ6677040.1"
                     /translation="GAEVAKALDCDVFTVDKDLVLGMLLEIPHLHPDDKERFKRNGML
                     FLASMKGRKT"
     gene            501..>685
                     /locus_tag="QBF46_004978"
     CDS             501..>685
                     /locus_tag="QBF46_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDQ6677041.1"
                     /translation="MKKTVLIKNIKSKKGFSLLELLLVLGIIAALVVAAFIVYPKVQA
                     SQRAQAESNNIATIQAGV"
BASE COUNT          228 a           93 c          123 g          241 t
ORIGIN      
        1 ggggctgaag ttgccaaggc tcttgattgt gatgttttta cggtggataa ggatcttgtt
       61 ttaggtatgc tactagagat accccaccta catccagatg ataaagaaag atttaaaaga
      121 aacggaatgt tatttctggc tagtatgaaa ggccgaaaaa cttaatggaa tatagaagtc
      181 attgtataag tataaaatgt tatatgtatt caactttcat tgaattaaat ttgacattat
      241 tttatattaa ttgtatttca ttttatcatc acagatgaat aactccatat tcaaatcttg
      301 taagctgtgg tggtatttaa taaagtcatg ggggtatcct cccgataaag cgaccgtaag
      361 tgaacacggt ctttttattt tctgttaagt aatccgctta tcggctactt tgtatataat
      421 attttttaag tggttgcgtt tatttttaaa tttattattt tctttatatc attttattaa
      481 acaatggaga tataaaggaa atgaaaaaaa cagtattaat taaaaacata aaaagtaaaa
      541 aaggtttctc acttttagaa ctattacttg ttcttggtat tatcgcagcg ttagtggtag
      601 cagcatttat cgtatatcct aaagtgcaag cgtctcaacg tgcacaggct gaaagcaata
      661 acatagcaac aatccaagct ggtgt
//
LOCUS       DAOFQD010000370          678 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.380, whole genome shotgun sequence.
ACCESSION   DAOFQD010000370 DAOFQD010000000
VERSION     DAOFQD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.380"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          225 a          128 c          119 g          206 t
ORIGIN      
        1 tgctggaaca gggctggccc ctgttaatga tggatttatc cataaagaac tgcggcagta
       61 gccgctgacc taataatagt ataattaatc ttatcaattc atattcgatt tagttcttaa
      121 gattttatat acgctagctt taccttgaaa ttaattaaaa cctcatattg aaaacagatc
      181 ttttcttatg atctttcgct catggctccg ccccttatct atatattaac gaggaatcaa
      241 tcaaggttat agcatttgct cttaaacgca tagtaaaact atatctccat agcagcactt
      301 cgtgattgtt caaaacaatc acattgctgg aacagggttc atccctgttg atgatggatt
      361 tatccatgaa taattgcggc gatagtcgct atgaggtaaa tgggaaacaa tttcaacttt
      421 aataagatat cagcagcgct tagtgattgt ttgaaacaat cacttcgctg agatataaat
      481 tgaagagagg aaaccaatgc aataaatagt taaactatac ccttgaaatg cctctgcata
      541 gttgttctca acttcactaa ttgaataatg aagaaggaaa atgctctcag ggcagctata
      601 gctcaaaacc aagtaccgac atccgggagt taaagtcccg aatcccggta cttaagttga
      661 taagtattct caaataaa
//
LOCUS       DAOFQD010000371          672 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.381, whole genome shotgun sequence.
ACCESSION   DAOFQD010000371 DAOFQD010000000
VERSION     DAOFQD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.381"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>672
                     /locus_tag="QBF46_004979"
     rRNA            <1..>672
                     /locus_tag="QBF46_004979"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          170 a          159 c          203 g          140 t
ORIGIN      
        1 aacgcgttaa gtcgaccgcc tggggagtac ggccgcaagg ttaaaactca aatgaattga
       61 cgggggcccg cacaagcggt ggagcatgtg gtttaattcg atgcaacgcg aagaacctta
      121 cctggtcttg acatccacgg aagttttcag agatgagaat gtgccttcgg gaaccgtgag
      181 acaggtgctg catggctgtc gtcagctcgt gttgtgaaat gttgggttaa gtcccgcaac
      241 gagcgcaacc cttatccttt gttgccagcg gtccggccgg gaactcaaag gagactgcca
      301 gtgataaact ggaggaaggt ggggatgacg tcaagtcatc atggccctta cgaccagggc
      361 tacacacgtg ctacaatggc gcatacaaag agaagcgacc tcgcgagagc aagcggacct
      421 cataaagtgc gtcgtagtcc ggattggagt ctgcaactcg actccatgaa gtcggaatcg
      481 ctagtaatcg tggatcagaa tgccacggtg aatacgttcc cgggccttgt acacaccgcc
      541 cgtcacacca tgggagtggg ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct
      601 taccactttg tgattcatga ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg
      661 cggttggatc ac
//
LOCUS       DAOFQD010000372          672 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.382, whole genome shotgun sequence.
ACCESSION   DAOFQD010000372 DAOFQD010000000
VERSION     DAOFQD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.382"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            35..672
                     /locus_tag="QBF46_004980"
                     /pseudo
     CDS             35..672
                     /locus_tag="QBF46_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          176 a          189 c          179 g          128 t
ORIGIN      
        1 taagtgagta aactctcagt cagaggtgac tcacatgaca aaaacagtat caaccagtaa
       61 aaaaccccgt aaacagcatt cgcctgaatt tcgcagtgaa gccctgaagc ttgctgaacg
      121 catcggtgtt actgccgcag cccgtgaact cagcctgtat gaatcacagc tctacaactg
      181 gcgcagtaaa cagcaaaatc agcagacgtc ttctgaacgt gaactggaga tgtctaccga
      241 gattgcacgt ctcaaacgcc agctggcaga acgggatgaa gagctggcta tcctccaaaa
      301 ggccgcgaca tacttcgcga agcgcctgaa atgaagtatg tctttattga aaaacatcag
      361 gctgagttca gcatcaaagc aatgtgccgc gtgctccggg tggcccgcag cggctggtat
      421 acgtggtgtc agcggcggac aaggataagc acgcgtcagc agttccgcca acactgcgac
      481 agcgttgtcc tcgcggcttt tacccggtca aaacagcgtt acggtgcccc acgcctgacg
      541 gatgaactgc gtgctcaggg ttaccccttt aacgtaaaaa ccgtggcggc aagcctgcgc
      601 cgtcagggac tgagggcaaa ggcctcccgg aagttcagcc cggtcagcta ccgcgcacac
      661 ggcctgcctg tg
//
LOCUS       DAOFQD010000373          667 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.383, whole genome shotgun sequence.
ACCESSION   DAOFQD010000373 DAOFQD010000000
VERSION     DAOFQD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.383"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          130 a          210 c          155 g          172 t
ORIGIN      
        1 gttgcatttc gctgattatc aaaggtcttc acctcgttag gtcagttaat tcacgtcgca
       61 aacacattca aactgcttcc cttcgccatg taatgggctt tccccatcgc ggactactac
      121 ggaagctccg ccagccagcg cgtcatcgga gccatgcccc cttaacatcc gtcgctgacc
      181 ttccccggtt tacctgcctg gactcaggca tactgaggag gctgcccgtc gcactcttta
      241 tccttgcttg ccgcaagttg gcagaagtca gcaacgcaag cgtgatagac gctgctgccc
      301 cggtgtttcg catacatgtc aaaacacctt cgaccggcag tgcttacgta tcactgccag
      361 ttcctcctgc acggcctgtc agatcacgta ggccgtggtg acgttttcaa cccacagagg
      421 cggattaacg ggttcatgtt cttcagcctt tcagtactta accttgagga tcatctcggc
      481 ttagtgatct cgcctcaatc cccgttgtca gcgggttaca tcaccctgcg ggcatgccgc
      541 aggtcactgc cgctcaggtt ctccaccgtc acacccggtg ggattgttgg gtttctcatc
      601 gtgagttacc ggttcaatat tccagacaga ctcgcggttc atttaagcat ccatgcccgc
      661 cctgaac
//
LOCUS       DAOFQD010000374          657 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.384, whole genome shotgun sequence.
ACCESSION   DAOFQD010000374 DAOFQD010000000
VERSION     DAOFQD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.384"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..573
                     /locus_tag="QBF46_004981"
     CDS             <1..573
                     /locus_tag="QBF46_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HDQ6677042.1"
                     /translation="NRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGLY
                     EFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRN
                     IKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYS
                     SYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT          241 a           92 c          143 g          181 t
ORIGIN      
        1 aaccgccacc gctattatga cccgctgcag gggcggtata tcactcagga tccgattggg
       61 ctgaaggggg gatggaacct gtatacatat ccgctgagcc cggtgaatag catggatcca
      121 ttaggattat atgaatttaa atcaaaaaat atagatgata ttggaatatt tgcattggca
      181 atgtgtaatg gagaatcaat taacgagaat aaagaatatg gtggactaat atgtaagaag
      241 caaggtgaat atttccccat gaatccgata agttcaaatg ataatgatag tgtagacttg
      301 cgaaatataa aatgccctga aggttcagag agagtaggcg attatcacac tcacggtttt
      361 tactctgacg ataaaggaaa taaagtaaca aaagaaaatg atgtttatga tagtctaaat
      421 ttttcaagca aagatttaac gaattcttat atgaatggaa tgggaaaaaa agaatacagt
      481 agttacttgg gaacaccaaa taacacctat ctaaaatata atcccaaagc taaagggaat
      541 ggagttacaa ttatcaggca agggagtaat taaaatgaag gcaatacaat gttgttattt
      601 ccttgcttta gtctccagtc atttaatggc tggagttatg ggtgatgata atatatt
//
LOCUS       DAOFQD010000375          635 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.385, whole genome shotgun sequence.
ACCESSION   DAOFQD010000375 DAOFQD010000000
VERSION     DAOFQD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.385"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            98..550
                     /locus_tag="QBF46_004982"
     CDS             98..550
                     /locus_tag="QBF46_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDQ6677043.1"
                     /translation="MTTGKDRLALINENTGEQVGNGGFYQAMEVDKTQFVKLYVDGVS
                     AIEGLSSSGKKVFKILYLAIRDNKDTDTILMSYDIVDQDVVKISRTTYFKGMKELAGK
                     KFIAETMIQNYYFINPDYMFNGDRLTFMKTYYLSSSNSNKPSNNKVKK"
BASE COUNT          236 a          104 c          114 g          181 t
ORIGIN      
        1 aactgaagta aagaaatcaa gaagaggtct cccacgtcat actaaaaacc catttcttaa
       61 tgatacaagt ctacatacaa aaactggagt cagacgtatt actacaggaa aagatcgatt
      121 agcattaatt aatgaaaata ctggagagca agttggtaat ggcggtttct atcaggctat
      181 ggaggttgat aagacacagt tcgtgaaact ttatgttgac ggagtatctg ctattgaagg
      241 actgtcttca tcgggtaaaa aagttttcaa gatcctttat cttgctatca gagataataa
      301 ggatactgat actattttga tgtcatacga tattgttgat caagatgtcg taaaaatatc
      361 cagaacaaca tatttcaaag gcatgaaaga acttgctggc aagaagttta ttgcagagac
      421 catgatacag aactattact tcattaatcc agactatatg tttaatggcg accgtttaac
      481 atttatgaaa acatattatc tttccagcag taactctaat aaacccagta ataataaggt
      541 gaagaaatga aatacttacc cggctcactc gaaataaaac tgcatgatag attatcaaaa
      601 accgatattc ttgatatctt agcggaacaa atgac
//
LOCUS       DAOFQD010000376          633 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.386, whole genome shotgun sequence.
ACCESSION   DAOFQD010000376 DAOFQD010000000
VERSION     DAOFQD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.386"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          193 a          131 c          112 g          197 t
ORIGIN      
        1 caccacctat catactgaaa taaaaggaga aaaaagaaag gtgaacagtg tgaacagttc
       61 tttcgaaaaa aaattttttt cctgagttaa cgtgcgtgaa gatacgtgta acaattaatc
      121 gatagatatt atatgtataa ggatgtgtat aaatgaatta gaatagagaa atataaacga
      181 tttgaatcaa atggttatat ggcaattttg actcctgtga tcttccgcca aaatgcctct
      241 tctgacgttc tccaacccct tgtaatactg tcaatccaga tattttctac tgctcaatat
      301 tcaatcggct tcaggaatac ctggtctaag gttttgtgat ggacgatgag cggctgaaga
      361 atccgcctgt cggttcttct gtcctcgaac gcatccgcga tattcacgcc agtatgcgcc
      421 gtgtttattt gcaggtgatt tgcccccctg ctgactctca gccaaccgta tcgccattaa
      481 ggtgaagatg ttgttttact cgccccagaa catccttttt tatacgacta ttctatttct
      541 ttaaccgctg gcgaaaaaaa ctgactataa acttcaccaa taatcagact gccatcaaga
      601 ttaatttgtt catttaatat atcaaatcaa ctt
//
LOCUS       DAOFQD010000377          632 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.387, whole genome shotgun sequence.
ACCESSION   DAOFQD010000377 DAOFQD010000000
VERSION     DAOFQD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.387"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(6..548)
                     /gene="fimA"
                     /locus_tag="QBF46_004983"
     CDS             complement(6..548)
                     /gene="fimA"
                     /locus_tag="QBF46_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDQ6677044.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          186 a          136 c          133 g          177 t
ORIGIN      
        1 tttgattatt catatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca
       61 cttgtaccgg tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag
      121 ttctggttgg ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt
      181 atttcaatac cgacacccgt tgctgttgtt gtattggtac tggatgcaat ggccaataaa
      241 ttatcattga ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca
      301 ggatcgcagt cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca
      361 ttgaaaatat cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc
      421 actgaacagg cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg
      481 gcagcataac tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt
      541 aatttcatat atattccttc aatctcattt atcgactcca catccgtata taaccgatta
      601 ctttatttaa gacactgata gtagtaaatt cc
//
LOCUS       DAOFQD010000378          621 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.388, whole genome shotgun sequence.
ACCESSION   DAOFQD010000378 DAOFQD010000000
VERSION     DAOFQD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.388"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>621
                     /locus_tag="QBF46_004984"
     CDS             <1..>621
                     /locus_tag="QBF46_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin cleavage protein"
                     /protein_id="HDQ6677045.1"
                     /translation="TSAGQLAFRVGFNSALYSIYLRRDGTLPMTGNLNMGGQSVYNAQ
                     NITANGTTTTGILKTNAAATVGTTLNVAGVTTLRSDLNVYGNGQIYGNLNSSNTVSGK
                     TVISRGDTYTQNWFRILGDGGLYFEKYGGRLTMLDDNTISAGESNLKTNSVLYATEIS
                     SGNINSAGTVTAERVDVVDYVWASGNISSNYVHSTGNLDADGQINAN"
BASE COUNT          209 a          101 c          123 g          188 t
ORIGIN      
        1 acctctgcag ggcaattagc tttccgtgta ggatttaata gcgcgttata ttctatctat
       61 ctccgtagag atggaacctt acccatgacc ggaaacctta atatgggtgg tcaaagcgtt
      121 tataatgctc agaatataac tgcgaatgga acaactacaa caggaatatt aaaaactaat
      181 gctgctgcta cagttggaac aacattaaat gtagcaggcg taactacttt aagatctgat
      241 ttaaatgttt atggaaatgg acaaatatat ggtaatttaa actcaagtaa taccgtttct
      301 ggaaaaactg taatatctcg tggcgatacc tatactcaaa actggttcag aatattaggt
      361 gatggaggtc tttatttcga gaaatatggg ggaagattaa ccatgctaga tgataacact
      421 ataagtgctg gagaatctaa ccttaaaact aattctgtat tatatgcaac tgaaatttct
      481 tctggtaata taaattctgc tggaacagta actgctgaaa gagtagatgt tgttgattac
      541 gtatgggcat caggaaacat cagctctaac tatgttcatt ctactggcaa tcttgatgct
      601 gatggtcaaa taaacgctaa t
//
LOCUS       DAOFQD010000379          621 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.389, whole genome shotgun sequence.
ACCESSION   DAOFQD010000379 DAOFQD010000000
VERSION     DAOFQD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.389"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            1..621
                     /locus_tag="QBF46_004985"
                     /pseudo
     CDS             1..621
                     /locus_tag="QBF46_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239532.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
BASE COUNT          241 a           89 c          113 g          178 t
ORIGIN      
        1 ccagaatggg gaggaattat tcgtaagttt aaagcaaaaa aaaataatag tgactgccac
       61 accataccga aacgacttat ttagttttga tattgataca gatcactcat atatatatac
      121 atacaaaaat gccatagaag aaggcataat atcagcacct gtttttgcaa aagcaaaaaa
      181 ggaagattta gtcagtgtag taagggatta cttaaacaaa aatagtgata cattgtgtat
      241 tgtcaagtgt gataatttta aaaacatatg cgaatattat aatcttttta aagatgaatt
      301 taaaacgctt gcgattcata gacaatttga gacttcacca ctggatgaga atttatataa
      361 tgtaccaagc aatttatctg aaagccctta taaaatacta attcatcaac gtatgttaga
      421 tgaaggtatc gatcttccga atgcaaaact actaatatta acctatagtg ttcggagtgg
      481 taaggaatta gtacaaacaa tggggcgtgt tgtaaggtgg tacaaagaca aaagcccact
      541 ggttatagac tacaatgagt gcaccaacgt tgatttatgg gagaactacc aagaatttga
      601 taattacttg acggataaaa c
//
LOCUS       DAOFQD010000380          610 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.390, whole genome shotgun sequence.
ACCESSION   DAOFQD010000380 DAOFQD010000000
VERSION     DAOFQD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.390"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>610
                     /locus_tag="QBF46_004986"
     CDS             <1..>610
                     /locus_tag="QBF46_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDQ6677046.1"
                     /translation="KDDNKIIVTTIQKLNNLMKAESDLPVYNQQVVFIFDECHRSQFG
                     EAQKNLKKKFKRYYQFGFTGTPIFPENALGSETTASVFGRELHSYVITDAIRDEKVLK
                     FKVDYNDVRPQFKSLETETDEKKLSAAENQQAFLHPMRIQEITQYILNNFRQKTHRTF
                     PGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAANKSATYKPLR"
BASE COUNT          194 a          132 c          134 g          150 t
ORIGIN      
        1 taaggatgat aacaaaatca tcgtcaccac tattcagaaa cttaataacc tgatgaaagc
       61 agaaagcgac ctgcctgtat acaatcagca agtggtgttt atatttgatg aatgtcaccg
      121 cagccagttt ggagaagcgc agaaaaacct gaagaagaaa ttcaaacgct attatcagtt
      181 tggttttacc ggtaccccta ttttcccgga aaacgcttta ggttcagaaa cgaccgccag
      241 cgtatttggt cgtgaattgc attcatatgt tattaccgat gcgattcgtg atgaaaaagt
      301 gctcaaattc aaggtggact ataacgatgt gcggccacag tttaagtctt tagagacaga
      361 aaccgacgag aaaaaactga gtgcggctga aaatcagcag gcgtttcttc atcccatgcg
      421 tatacaggaa attacgcaat atattctgaa taacttccgc cagaagaccc accgtacttt
      481 cccaggttcc aaaggcttta atgccatgct ggcagtgagt agcgtggatg ccgcgaaagc
      541 ctattacgcg acgtttaaac ggttacaaga ggaagcagct aataaatcgg ccacctataa
      601 accgctgcgt
//
LOCUS       DAOFQD010000381          601 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.391, whole genome shotgun sequence.
ACCESSION   DAOFQD010000381 DAOFQD010000000
VERSION     DAOFQD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.391"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..320)
                     /locus_tag="QBF46_004987"
     CDS             complement(<1..320)
                     /locus_tag="QBF46_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6677047.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNALAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLST"
     gene            complement(362..526)
                     /locus_tag="QBF46_004988"
                     /pseudo
     CDS             complement(362..526)
                     /locus_tag="QBF46_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="involved in type 1 fimbriae biosynthesis, interacts
                     with FimH; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone protein FimC"
BASE COUNT          165 a          126 c          133 g          177 t
ORIGIN      
        1 tctgtactca ggcatgggat gagttctgca ttattatcat ctgcaataaa agtaatatcc
       61 cgactagtca taaattcatc gttcaaataa atgtcgaccc gatatgtacc aggtggtgcc
      121 tcgcgtccct tagtaaaggc ggataaatcg acagattcag caagagcatt gcttaaaaac
      181 cgtggattaa aataatactc tgcgtcaacg ggaaataccg cagttgctaa gccaagggca
      241 atggtaagcc gtgcaaagcg aggcattatt aacacctgat gcatacgata tgccttaaat
      301 gcggcacgaa tggctgtcat tattaattcc gattgagttt attattaatt aggtcactgg
      361 cttactgcat ggttgcctta atacgcggag ttaatgcacc gtaatcattg atcgtctgaa
      421 aactgatatc cccggttgca gcatgcgtga tatccaccga tacttcacct tttggcggca
      481 ccatggtgtt tgggagattg ctatttcctg ctttcatatt tgttacggct ttgttgaata
      541 aataagattt cgtgcgaacg accctgtagc tggctggatt ttcaggcaat acgcacgctt
      601 t
//
LOCUS       DAOFQD010000382          598 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.392, whole genome shotgun sequence.
ACCESSION   DAOFQD010000382 DAOFQD010000000
VERSION     DAOFQD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.392"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..407
                     /gene="radC"
                     /locus_tag="QBF46_004989"
     CDS             <1..407
                     /gene="radC"
                     /locus_tag="QBF46_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDQ6677048.1"
                     /translation="ALDRHLHEPGVAFTSTHAVREWLRLHMAALEREEFRVLYLDNQN
                     QLIAHETLFTGTINRTEVHPREVVKRALHFNAAAVILAHNHPSGETTPSQADKTLTQR
                     LVQVLQLVDIRVPDHLIVGGRQIYSFAEHGLL"
     gene            416..>598
                     /locus_tag="QBF46_004990"
     CDS             416..>598
                     /locus_tag="QBF46_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDQ6677049.1"
                     /translation="MKIISKRRAMTIYRQHPESRIFRYCTGKYQWHGSVCHYTGRDVP
                     DIAGVLAVYAERRQDRN"
BASE COUNT          132 a          181 c          163 g          122 t
ORIGIN      
        1 gggctctgga ccgccacctg catgagcccg gcgtagcctt cacctctacc cacgccgtac
       61 gtgaatggct gcgactgcat atggccgcgc ttgagcggga agagttccgg gtgttgtatc
      121 tggacaacca gaatcagttg attgcccatg aaacgctctt caccggcacg attaaccgca
      181 ccgaggtgca tccccgggag gtggtcaaac gtgctctgca cttcaacgcg gcggcggtga
      241 tactcgcgca taaccatcct tccggcgaga cgacacctag ccaggccgac aaaaccctca
      301 cgcagcgact ggttcaggtg cttcagctgg tggatatccg tgtccctgac catctgattg
      361 tcggtggcag gcaaatctat tcgttcgcag aacacggtct gctttgaggt attacatgaa
      421 aattatcagt aaacgcaggg caatgacgat ataccgccag catcctgagt cccgaatctt
      481 tcgctactgc accggcaaat accagtggca cggtagcgtc tgtcattaca ccggcaggga
      541 cgttccggat atcgccggag tcctcgcggt atacgccgaa cgccgccagg accgcaat
//
LOCUS       DAOFQD010000383          597 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.393, whole genome shotgun sequence.
ACCESSION   DAOFQD010000383 DAOFQD010000000
VERSION     DAOFQD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.393"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            81..374
                     /locus_tag="QBF46_004991"
     CDS             81..374
                     /locus_tag="QBF46_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677050.1"
                     /translation="MSKIFEIKSVSTETFYNIAERSFEASWKVMQDMASDNVSYLVYD
                     ADFMCVFIGNVIEHISKNFYIIIQCECLEGKLEEVNFEEVAERLVRHSWEFCK"
BASE COUNT          202 a           75 c          125 g          195 t
ORIGIN      
        1 taataaatca tttttagcct cattcatggg gcgagaaaat aaaaaagcaa acaaatatca
       61 tttaatagtg aggcgtaacc atgagtaaaa tttttgaaat taaaagtgta tctacagaaa
      121 cgttttataa tattgcagag cgttcgtttg aggctagttg gaaggttatg caggatatgg
      181 cctctgataa tgtctcatac ttggtatatg atgcggattt tatgtgtgtt ttcatcggca
      241 atgttattga gcatatcagt aaaaattttt acatcattat ccaatgtgaa tgtctggaag
      301 gaaagcttga agaagtaaac tttgaagagg tagcggaacg tctggtccgg cattcatggg
      361 agttttgtaa ataatatcaa aataaaaata gattaagaaa tcatgggcag agttgttatc
      421 tactcactga tttagattat gtggccccgt aatggggctg tacatcgatt acaagaggaa
      481 tatgatatga atgatgtggt attatacgaa aaaaatgaat caatgtcttt tgccatttgc
      541 acggtcttat cattatattg cgatttcata tacgaaatag catatggatt tcataat
//
LOCUS       DAOFQD010000384          596 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.394, whole genome shotgun sequence.
ACCESSION   DAOFQD010000384 DAOFQD010000000
VERSION     DAOFQD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.394"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..476
                     /gene="espX1"
                     /locus_tag="QBF46_004992"
     CDS             <1..476
                     /gene="espX1"
                     /locus_tag="QBF46_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HDQ6677051.1"
                     /translation="KSNLSYGYYFGCVLSNISCFESDLSNTILSNGEINNLFIKKSNI
                     FGTSFTNTRIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIP
                     LVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLKSINECMFLMKNIAHQN"
BASE COUNT          235 a          104 c           90 g          167 t
ORIGIN      
        1 aaaagagcaa cctttcatat gggtattatt ttggttgtgt attatccaat atatcatgtt
       61 ttgaaagcga cttatcaaac accatcttat caaatggaga aataaacaac ctcttcatta
      121 aaaaaagcaa catatttgga acatcgttca ctaataccag gataaaaaat ttgcgctgtg
      181 aagatattat gcctggccga tggaccactc aacttgtcaa taagcactta ggttacagat
      241 acacaggagt attcaaaacc ctggcaagta ttgatgacaa accgtcccgt ttcgaaattt
      301 tgataccatt ggtacaaact ttagttcgcg acaatgtaaa gctaaataat gatgtgtata
      361 aagaactgaa aaagtttatg cacgattacg acaaaacatc cccagaaatg agaaaatacc
      421 ttaaaagcat caacgagtgt atgttcctta tgaaaaacat agctcatcaa aattaaaaaa
      481 tctcaaaaaa gaagtaattt cagaatgaaa ttaactgtga tgattattaa ttcactacag
      541 aggaataaaa aataatctga agcatgctct gaaacatcct actctatcat tctaat
//
LOCUS       DAOFQD010000385          593 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.395, whole genome shotgun sequence.
ACCESSION   DAOFQD010000385 DAOFQD010000000
VERSION     DAOFQD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.395"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..482)
                     /gene="fimE"
                     /locus_tag="QBF46_004993"
     CDS             complement(<1..482)
                     /gene="fimE"
                     /locus_tag="QBF46_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDQ6677052.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     G"
BASE COUNT          175 a          154 c          126 g          138 t
ORIGIN      
        1 ccgagataat cctgaattaa acgggtatct gcaccacgct ccgccagttc ataaccgcaa
       61 gcatgcctta acatatgagg atgagtctgc gttacggttc cagcttcaat accggcatcg
      121 cgaataatgc gataggcctg ctggcgagaa agccgactcc cgcggcgaga aataaatata
      181 gcgtcggtcc ggtcagcgcc tttccagtta gcacgttcct gggtccagcg ttccacggct
      241 tcacgctcat caaaacgtaa cgggtgaacg gtagaaaatc cgttcttcag tcggcgaata
      301 tttattcgac cttcattaag gtcaaggtcc tgataatgca gatcaagcag ttcactaata
      361 cgcatcccat gccgatatgc caacagaata agacaataat ctctggctcc cgttgccccg
      421 taacaaaccg cctgcatcat ggcctgaact tctttaccgg taagataacg acgtttactc
      481 acaatagtag tactcctgac tgagatatat ttgaatgaat acctatagga aacctcaatc
      541 ggtcaaaatt agcccaatag ataaaaagat aaccacacac gaagtgatgt ggc
//
LOCUS       DAOFQD010000386          591 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.396, whole genome shotgun sequence.
ACCESSION   DAOFQD010000386 DAOFQD010000000
VERSION     DAOFQD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.396"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          170 a          111 c          123 g          187 t
ORIGIN      
        1 tcttgtatca ggctcaccta acttagagag taattccgtc ttagttgttt tgtttttaat
       61 gatctttgac tggaggcttt gtggtgtttc gtttttaagg ttctggttcc ctgatgttga
      121 acagccagaa agcaggaaga caataataaa aaaagaggac gttataaatc ggttcattta
      181 ctttctcatt ttatgtttgc gattgtgtga aattaatttc accgtataaa aacaactctg
      241 tctgcataca ttcttttgat tttatagata agaataatga tgcttacgaa aaacgtaatg
      301 actgatacaa gagtgctgtc ggtccacgaa aatgctaacc aggcgagacc aaatgccacg
      361 aattgaaaca ggaatgccac actgagcggg ctttcaccgt aagcaacctt tgcatgacac
      421 cctttgcaaa tccgcacacc atgaggactt tgtgtaaagc agaacgggca ctttattgtt
      481 tcgtctttca tatcaacccc agggtggaag attaaatggt gcgtttacag agattctcga
      541 tgaccggctc gctttagtaa aattgcttgt accgatcaat ggttgttact t
//
LOCUS       DAOFQD010000387          589 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.397, whole genome shotgun sequence.
ACCESSION   DAOFQD010000387 DAOFQD010000000
VERSION     DAOFQD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.397"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..562
                     /locus_tag="QBF46_004994"
     CDS             <1..562
                     /locus_tag="QBF46_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6677053.1"
                     /translation="IFIRSSELRFARWSEIDFKSKLWVIPEQREAIENVKHSTRGAKM
                     KRKHFVPLCKQAMKILKEIRKLTYEEGHDDGLIFTGCYDSFKPMSENTINKALRNMGY
                     DTKQDICGHGFRTLACSALIESGLWSEDAVELQMSHKESNSVRAAYTHKAKHLDQRRL
                     MLQWWADFLDANSNDMVRPFEFASNK"
BASE COUNT          169 a          130 c          143 g          147 t
ORIGIN      
        1 gattttcatt cgttccagtg agttgcgttt cgctcgctgg tcagaaattg atttcaaaag
       61 taagttatgg gtgatacccg aacagcgtga agcaattgaa aacgtcaaac attcgactcg
      121 tggggctaaa atgaagcgta agcacttcgt tcccctttgt aagcaggcca tgaagatact
      181 caaagagatc cgaaaactga cttatgaaga aggtcatgat gacggattaa tctttactgg
      241 ttgttatgac tcgtttaaac ccatgagcga aaacaccatc aacaaagcgc ttcgcaatat
      301 gggctatgac acaaagcagg acatctgtgg acacggtttc cgcactctgg cctgtagtgc
      361 cttaattgag tccgggctat ggtcagaaga cgctgtagag cttcaaatga gccataagga
      421 aagcaatagc gtccgtgctg cttataccca caaggcaaaa caccttgacc agcgccgcct
      481 gatgctccag tggtgggctg atttccttga tgctaacagc aacgatatgg tcaggccgtt
      541 tgagtttgct tcaaataaat aaagggaaag gccgttcgcg gcctttcaa
//
LOCUS       DAOFQD010000388          579 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.398, whole genome shotgun sequence.
ACCESSION   DAOFQD010000388 DAOFQD010000000
VERSION     DAOFQD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.398"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..269)
                     /locus_tag="QBF46_004995"
     CDS             complement(<1..269)
                     /locus_tag="QBF46_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HDQ6677054.1"
                     /translation="MTQGYELVVKGVRNFENKVTVTVALQDKERFDGEIFGLDVAMDR
                     VEGATLEFYEAAARMSVRQVFLEVAEKLSEKLSLICSISTPLRLK"
     gene            complement(274..489)
                     /gene="essD"
                     /locus_tag="QBF46_004996"
     CDS             complement(274..489)
                     /gene="essD"
                     /locus_tag="QBF46_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDQ6677055.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT          153 a          154 c          109 g          163 t
ORIGIN      
        1 attttcaatc ttaaaggagt actgatgctg cagataagac tcaacttttc tgacaatttt
       61 tctgctactt ccaggaagac ttgccggacg ctcattctgg ctgctgcctc ataaaactcc
      121 agcgtagctc cttcaacacg gtccatggcg acatccaggc caaaaatttc accgtcaaag
      181 cgttctttgt cctgtaaggc tacagttacc gtaactttat tctcaaaatt acggactcct
      241 ttcacaacca gttcatagcc ttgagtcatt ggattattct cctctcgcag ccttacgcct
      301 gtcttcttta atcttgaaat aaagatttgt cagatacgtc agcaggccaa aaaccaggct
      361 acccagcaca ccgattgcag cccactgtga cggagttact ttatcgagta actgcaatgc
      421 ccagaaacca gcattacccg ccgatgtgcc ataggcaaca cctgttgtta acttatccat
      481 tgatttcata tcctcacccc gatgtacacg gatggtgcaa tatgtttgaa aagatcggag
      541 tctacggggt agttttgaca gcacacgctg ttctcaacg
//
LOCUS       DAOFQD010000389          578 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.399, whole genome shotgun sequence.
ACCESSION   DAOFQD010000389 DAOFQD010000000
VERSION     DAOFQD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.399"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>578
                     /locus_tag="QBF46_004997"
     rRNA            <1..>578
                     /locus_tag="QBF46_004997"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          132 a          130 c          191 g          125 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ccctttaagg gtcctgaa
//
LOCUS       DAOFQD010000390          571 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.400, whole genome shotgun sequence.
ACCESSION   DAOFQD010000390 DAOFQD010000000
VERSION     DAOFQD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.400"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            47..265
                     /locus_tag="QBF46_004998"
     CDS             47..265
                     /locus_tag="QBF46_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677056.1"
                     /translation="MSLTEKRFLDDAVHAATKVKGKKLTSEERRKVLCIAREQIRSQR
                     RAEEIKKERDKERHAVVFEWKRPETFRR"
     gene            292..>571
                     /locus_tag="QBF46_004999"
     CDS             292..>571
                     /locus_tag="QBF46_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677057.1"
                     /translation="MNKTKTKNLKESEFIKYIENNPVVSTALCEKYGISTETIAYALY
                     VHSNSFFLKNNYVQDDIHYDMVKNNLIIMKPTLKQYYRRTRQVDYNGCT"
BASE COUNT          240 a           80 c          106 g          145 t
ORIGIN      
        1 taaattaaaa aacgaaagaa aacaccgtgc tggaagatta cagagagtgt cattaacgga
       61 aaagagattt cttgatgatg ctgttcatgc cgctactaaa gttaaaggaa aaaaattaac
      121 atcagaggaa cgtaggaaag tattatgtat agcgcgtgaa cagatccgtt ctcagcgtag
      181 agcagaagaa ataaaaaaag aaagagacaa agaacgtcat gcagtagtgt ttgagtggaa
      241 aagaccagaa accttccgtc gttaatcgga tataataata gtgagaaata tatgaataaa
      301 accaaaacaa aaaacttgaa agaaagtgag tttataaaat atatagaaaa caatcctgtt
      361 gtctcaacgg ctttatgcga aaaatatgga atatctactg aaaccattgc ctatgcttta
      421 tatgttcata gcaatagttt ctttcttaaa aacaactacg ttcaggatga cattcactat
      481 gacatggtta aaaataattt aataataatg aagcccacgc ttaaacaata ttatagaaga
      541 acaaggcaag tagattataa cggatgtact t
//
LOCUS       DAOFQD010000391          571 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.401, whole genome shotgun sequence.
ACCESSION   DAOFQD010000391 DAOFQD010000000
VERSION     DAOFQD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.401"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..>571)
                     /locus_tag="QBF46_005000"
     CDS             complement(<1..>571)
                     /locus_tag="QBF46_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDQ6677058.1"
                     /translation="HIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGC
                     GWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKV
                     QNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLR
                     LRGWQVPAFTLGGEATDIVVMRIMCRRGFE"
BASE COUNT          135 a          168 c          162 g          106 t
ORIGIN      
        1 tttcgaagcc gcgacgacac ataatgcgca tcaccacgat gtcggtggct tcaccgccga
       61 gagtgaaggc cggaacctgc cagccgcgca gacgcagacg ttcagagagg tcgtacaggg
      121 tgtatcccgg atcttcacca tctttcagtt tgaagcaaac cgccgggatg ccttcgtccg
      181 ggcgacccgt acagatgaac tcatacggcc ccagtttggc gatttcatcc gccagataag
      241 cggcaacctg gtaagaggcg ttctgtactt tggtatagcc ttcacgaccg aggcgcagga
      301 attcatagta ctgtgcaatt acctgacccg ccgggcggga gaagttgatg gcaaaagtac
      361 caatttgacc acccaggtag tcaacgttga acaccagttc ctgcggcagc gcttcttcgt
      421 cacgccagat aacccagccg cagcccagcg gagccagacc gaatttatgg cctgaagcac
      481 tgatcgattt cacacgcggc aggcggaagt cccagacgat atccggggcg acgaacggtg
      541 ccaggaagcc accgctggca gcgtcgatgt g
//
LOCUS       DAOFQD010000392          554 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.402, whole genome shotgun sequence.
ACCESSION   DAOFQD010000392 DAOFQD010000000
VERSION     DAOFQD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.402"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            169..>554
                     /locus_tag="QBF46_005001"
     CDS             169..>554
                     /locus_tag="QBF46_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677059.1"
                     /translation="MTTQKEMEEIRAALSWFDVRRYDGLKDLTLEQIYAELERRMLAY
                     KTRQQWETAGKDNQMQAIYHDATIRCGDVILKSDWISDNHRLSHSYAVRPMTRVQLFN
                     YGRAMYRLETSEQTDPVAVSSDYISE"
BASE COUNT          181 a          113 c          118 g          142 t
ORIGIN      
        1 attggttatt ttttattaaa taaatcatgt ttagtcaagg tgaaagcact gacctccaag
       61 gtcacttact gtgctcacac cattttgaaa acccgctcct cttttccact ttctctatca
      121 tgttgaatgg gctaacgttt aggaaacaca aaagcaatcg gtagaacaat gactacacaa
      181 aaggaaatgg aagaaatcag ggcagcgtta tcttggtttg atgtacgccg atatgatggc
      241 ctgaaagacc tcaccttaga acaaatctat gcagagcttg aaagacggat gttggcttac
      301 aaaacaagac aacaatggga aacagcaggc aaagataatc aaatgcaagc gatataccat
      361 gatgccacaa tccgatgtgg tgatgtgata cttaaatcag actggatttc agacaatcac
      421 agactgtcgc atagctatgc tgtaagacca atgaccagag ttcaattgtt caactatggg
      481 cgggctatgt atcggctgga gacttcagag caaacagacc ctgttgcagt gagttccgac
      541 tacatctctg agta
//
LOCUS       DAOFQD010000393          554 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.403, whole genome shotgun sequence.
ACCESSION   DAOFQD010000393 DAOFQD010000000
VERSION     DAOFQD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.403"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..529
                     /locus_tag="QBF46_005002"
     CDS             <1..529
                     /locus_tag="QBF46_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6677060.1"
                     /translation="RWSEIDFKSKLWVIPEQREAIENVKHSTRGAKMKRKHFVPLCKQ
                     AMKILKEIRQLTYEEGQDDGLIFTGCYDSFKPMSENTINKALRNMGYDTKQDICGHGF
                     RTLACSALIESGLWSEDAVELQMSHKESNSVRAAYTHKAKHLDQRRLMLQWWADFLDA
                     NSNDMVRPFEFASNK"
BASE COUNT          159 a          127 c          136 g          132 t
ORIGIN      
        1 tcgctggtct gaaattgatt tcaaaagtaa gttatgggtg atacccgaac agcgtgaagc
       61 aattgaaaac gtcaaacatt cgactcgtgg tgccaaaatg aagcgtaagc acttcgttcc
      121 cctttgtaag caggccatga agatactcaa agagatccga caactgactt atgaagaagg
      181 tcaagatgat gggttaatct ttactggctg ttatgactcg tttaagccca tgagtgaaaa
      241 caccatcaac aaagccctcc gcaatatggg ctatgacacg aagcaggaca tctgtggaca
      301 cggtttccgc actctggcct gtagtgcctt aattgagtcc gggctatggt cagaagacgc
      361 tgtagagctt caaatgagcc ataaggaaag caacagcgtc cgtgctgctt atacccacaa
      421 ggcaaaacac cttgaccagc gccgcctgat gctccagtgg tgggctgatt tccttgatgc
      481 taacagcaac gatatggtca ggccgtttga gtttgcttca aataaataaa gggaaaggcc
      541 gttcgcggcc tttc
//
LOCUS       DAOFQD010000394          544 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.404, whole genome shotgun sequence.
ACCESSION   DAOFQD010000394 DAOFQD010000000
VERSION     DAOFQD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.404"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..377
                     /gene="agaB"
                     /locus_tag="QBF46_005003"
     CDS             <1..377
                     /gene="agaB"
                     /locus_tag="QBF46_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDQ6677061.1"
                     /translation="VVDDVVANDDIQQKLMGITAETYGFGIRFFTIEKTINVIGKAAP
                     HQKIFLICRTPQTVRKLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFI
                     KQRGVNVFIQDVPGDQKEQIPD"
     gene            416..>544
                     /locus_tag="QBF46_005004"
     CDS             416..>544
                     /locus_tag="QBF46_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane;protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IIC forms the translocation channel for the sugar
                     uptake; involved in N-acetylgalactosamine transport;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDQ6677062.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLIGAI"
BASE COUNT          156 a          108 c          120 g          160 t
ORIGIN      
        1 tagtcgtgga tgatgttgtc gctaacgatg atattcaaca gaaattaatg ggtattaccg
       61 cggaaaccta cggctttggc attcgtttct ttactatcga aaaaaccatt aacgtcatcg
      121 gcaaagctgc accgcatcag aagatcttcc tgatttgccg tacgccacaa acggtacgta
      181 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
      241 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      301 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      361 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      421 tgaaataacc ctacttcagg gattatccct ggcggcgtta gtttttgttc tggggattga
      481 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taattggcgc
      541 tatt
//
LOCUS       DAOFQD010000395          540 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.405, whole genome shotgun sequence.
ACCESSION   DAOFQD010000395 DAOFQD010000000
VERSION     DAOFQD010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.405"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(114..>540)
                     /locus_tag="QBF46_005005"
     CDS             complement(114..>540)
                     /locus_tag="QBF46_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677063.1"
                     /translation="YNHVGYEFKSSEKDFYPLIEPIFKQNSLPYSKLSYKVFDLSFLT
                     NEGTTHKEKKNVATKDLRDDLFNKEGIKAVGSIGLYRIGIRVDRNNPKLQLADNVELI
                     IPGTLRRHLGGSSCSPVNYAILSKCISKDDFETLTKLIL"
BASE COUNT          158 a          116 c           81 g          185 t
ORIGIN      
        1 cttgcctcga tttcatcgta caaatagcaa gctaacgccc caatttcagg gcgttctgca
       61 cacatttctt tgatgatctt gggcatatca ccatccttca ttgctgactc caattataat
      121 atcagctttg tgagggtttc gaaatcatct ttggatatac atttacttaa tatagcatag
      181 ttaacaggag agcaactact ccctcctaga tgcctgcgga gtgttcccgg aatgataagc
      241 tctacgttat ccgctaattg cagttttgga ttatttctat caactctaat tccaatacga
      301 tacaaaccaa tactgcctac tgctttgatg ccttccttat taaacaaatc atcacgtaag
      361 tctttagtgg ccacattttt cttttcttta tgcgtagtcc cctcattcgt caaaaatgac
      421 aaatcaaaga ctttatagct taattttgaa taaggcaatg aattttgttt gaaaattggc
      481 tcaattaacg gataaaaatc tttttccgat gatttaaatt cataaccgac atgattatac
//
LOCUS       DAOFQD010000396          526 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.406, whole genome shotgun sequence.
ACCESSION   DAOFQD010000396 DAOFQD010000000
VERSION     DAOFQD010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.406"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>526
                     /locus_tag="QBF46_005006"
     CDS             <1..>526
                     /locus_tag="QBF46_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDQ6677064.1"
                     /translation="ECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISA
                     FSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGG
                     DYLFAVKGNQGRLNKAFEEKFPLKELNNPAHDCSAISEKSHGREEIRLHIVCDVPDEL
                     IDFTFEWKGLKKLCV"
BASE COUNT          175 a           92 c          123 g          136 t
ORIGIN      
        1 gagtgcttta ttaactggat gcgtgactgc cattcttcag atgataaaga cgtcatcgca
       61 attgatggaa aaacactccg gcactcttat gacaagagtc gccgcaaggg agcgattcat
      121 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag
      181 aaatctaatg agattacagc tatccctgaa cttcttaaca tgttagatat taaaggaaaa
      241 attatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
      301 ggaggtgatt atttattcgc tgtaaaagga aaccaggggc ggcttaataa agcctttgag
      361 gaaaaatttc cgctgaaaga attaaataat ccagcgcatg actgttccgc aattagtgaa
      421 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      481 attgatttca cgtttgaatg gaaagggctg aaaaaattat gcgtgg
//
LOCUS       DAOFQD010000397          507 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.407, whole genome shotgun sequence.
ACCESSION   DAOFQD010000397 DAOFQD010000000
VERSION     DAOFQD010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.407"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..>507)
                     /locus_tag="QBF46_005007"
     CDS             complement(<1..>507)
                     /locus_tag="QBF46_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6677065.1"
                     /translation="ISKFLYEHNLVKSGYISWKNHVKQKVKNNYLPEIEDYHRSDKLP
                     DEEALLAIADIFSQNDELLSPRDKFTSSVFALLLCCPSRISEILALPADCEITQIDGK
                     GIERYGLRFYSVKGYGPNIKWIPRVMIPVAKKAIRRLLSLSQNARALAYWCEKYPDKF
                     YRHELCPTV"
BASE COUNT          149 a          103 c           86 g          169 t
ORIGIN      
        1 actgttgggc aaagctcatg tcggtaaaat ttatccgggt acttttcgca ccagtaagca
       61 agtgctcttg cattttgtga taaggaaagt aatcttctaa ttgctttctt tgcaactggt
      121 atcataaccc gtggaatcca tttgatatta gggccatacc cttttaccga ataaaatctc
      181 aacccatatc tttcgatacc cttgccatct atttgtgtaa tctcacaatc agcaggtaag
      241 gctaaaatct cagagattct gctcggacaa caaagtagaa gtgcaaatac tgaacttgtg
      301 aacttatccc ttggactcag taactcatca ttttgagaaa aaatatcagc aatagcgagc
      361 aatgcctctt catctggtaa cttatcgctt cggtgataat cctcaatctc aggaagataa
      421 ttgtttttga ctttctgttt tacatggttt ttccatgaga tatatcctga cttcacaaga
      481 ttatgttcat atagaaactt tgatatt
//
LOCUS       DAOFQD010000398          503 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.408, whole genome shotgun sequence.
ACCESSION   DAOFQD010000398 DAOFQD010000000
VERSION     DAOFQD010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.408"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..474
                     /locus_tag="QBF46_005008"
     CDS             <1..474
                     /locus_tag="QBF46_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDQ6677066.1"
                     /translation="GKKVREDNPEWKGDVGFRVFKLDTSNIRPWEATAETLSEQLDAY
                     VSPILEGRSEEDLLTELMLKRGIDLSVNVEVRQFDGLTVSCVDGGKLFTCFAKQIPAS
                     SVEELTKGIIDWHKSLKAGKDTVCYFLDDAFENNIAKTNLCAILEQHGLTNLHCL"
BASE COUNT          145 a           98 c          117 g          143 t
ORIGIN      
        1 ggtaaaaagg ttcgtgaaga caatccagag tggaaaggtg atgttggttt tcgtgttttc
       61 aaactcgata catctaacat tcgtccgtgg gaagccacag cagaaaccct ttcagaacag
      121 cttgatgctt atgtaagccc aatccttgaa ggccgtagtg aagaagactt gctgactgaa
      181 ctgatgctta agcgtggtat tgatctcagc gtgaacgttg aagttcgcca gtttgatgga
      241 ttaaccgttt cttgtgttga cggtggtaaa ttgtttacct gctttgctaa acaaatccct
      301 gcatcttcag tagaagaact gaccaagggg atcatcgact ggcataagag tttgaaagct
      361 ggcaaagata ctgtctgcta tttccttgat gacgcatttg aaaataatat tgctaaaact
      421 aatctttgcg ccattctgga acaacacggc ctgactaacc tgcactgcct gtaagggaat
      481 gagatgaaac tacattttga atc
//
LOCUS       DAOFQD010000399          500 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.409, whole genome shotgun sequence.
ACCESSION   DAOFQD010000399 DAOFQD010000000
VERSION     DAOFQD010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.409"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>500
                     /locus_tag="QBF46_005009"
     CDS             <1..>500
                     /locus_tag="QBF46_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpnT protein"
                     /protein_id="HDQ6677067.1"
                     /translation="NIYNRVEKNNDSLDLFSGIAKKKGFPFVILFLSGTDSYGKLDSE
                     VMNASENEIAEMMSLLRGSFVRTLSSELYRHGYACSATLLRRVLAEDSISRSQSKYYS
                     YAASDMKKSIDYSKDITWTEKIPSTEEYLKSLFIEHKRKYALWEIMLEKIAGLAIEKD
                     SVSYSA"
BASE COUNT          165 a           82 c          107 g          146 t
ORIGIN      
        1 ataatatcta taatcgggta gagaaaaata atgattcact ggatttgttc tctggcatag
       61 caaagaaaaa aggctttccg tttgttattc ttttcttgtc cgggacagat agttatggaa
      121 aacttgattc tgaagtcatg aatgcgagtg aaaatgaaat cgcagagatg atgtctttat
      181 tacgcggttc ttttgttcgt accttatcga gtgagcttta caggcatggc tatgcatgtt
      241 ctgcaactct gctccgtaga gtgctggcag aggacagtat cagccgttct cagagtaaat
      301 actacagcta tgcggcatct gatatgaaaa aaagcattga ttacagtaaa gatattacat
      361 ggactgaaaa aatcccttca acagaagagt atttaaaatc cctgtttatt gagcacaaaa
      421 gaaaatacgc actctgggaa ataatgctgg aaaaaatcgc tggtttagcg atagagaaag
      481 attcagtatc atattcagca
//
LOCUS       DAOFQD010000400          498 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.410, whole genome shotgun sequence.
ACCESSION   DAOFQD010000400 DAOFQD010000000
VERSION     DAOFQD010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.410"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          145 a           87 c          111 g          155 t
ORIGIN      
        1 gtactggagc tattagtggc tctgttacgg ttgattgatt tgttgaagta acgggcaaat
       61 tgaaacgcat ctaaggggga aaactgcttc cccctttaat aaaatgatta ctgtttttat
      121 taattcatca tataactcat ttttatttat aaattcatcg attttatatt caacgtaccg
      181 tttgaccggg atgtgaatat ttatggtggg ctattggcaa aaaatatctg gaaagtagat
      241 cgcagaaaat aaaaaaacct gctggtgagg caggttcttt tatttactac tttgatgtta
      301 agttactgat agtgcaacat ggcatctcaa cgaacgaatt ataaccacct ctggacagtg
      361 ttgcaattaa gataatgaga tttataatcc tgactgttgg gaagttgcgc cggatcgaac
      421 ccatttcaag tcctggctac cggacgggtg gtgccgcagg tagtgctctg atccggagtg
      481 caacatggcg actcaaca
//
LOCUS       DAOFQD010000401          494 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.411, whole genome shotgun sequence.
ACCESSION   DAOFQD010000401 DAOFQD010000000
VERSION     DAOFQD010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.411"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>494
                     /locus_tag="QBF46_005010"
     CDS             <1..>494
                     /locus_tag="QBF46_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDQ6677068.1"
                     /translation="GMFLTGFDAPTLNTLFVDKNLRFHGLMQAFSRTNRIYDATKTFG
                     NIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGFTDAATGEAKRGFMAVVSE
                     LEQRFPDPASIESEKEKKDFVKLFGEYLRAENILQNYDEFATLKALQQIDLSDPVAVE
                     KFKA"
BASE COUNT          151 a          110 c          110 g          123 t
ORIGIN      
        1 gggatgtttt taaccggctt cgacgctcca acattgaaca cgctattcgt cgataaaaac
       61 ttgcgttttc acggcctgat gcaggcattc tcccgcacca accgcattta tgacgctact
      121 aaaaccttcg gtaacatcgt cactttccgg gatctggaac gctcaaccat tgatgccata
      181 acgctgtttg gtgataaaaa taccaaaaat gtggtattag aaaagagtta tgcagagtat
      241 atggaaggct ttacagatgc tgccactggt gaagctaaac gcggctttat ggcagtagtt
      301 tcagaactgg aacaacggtt ccctgaccct gccagtattg aaagtgaaaa agagaagaaa
      361 gacttcgtaa aactgtttgg tgaatacctg cgtgccgaga acatcctgca aaactatgat
      421 gaatttgcca cgctgaaagc cctgcaacaa atcgatctta gcgatcctgt tgcggtagaa
      481 aaattcaaag caga
//
LOCUS       DAOFQD010000402          493 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.412, whole genome shotgun sequence.
ACCESSION   DAOFQD010000402 DAOFQD010000000
VERSION     DAOFQD010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.412"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            38..451
                     /locus_tag="QBF46_005011"
                     /pseudo
     CDS             38..451
                     /locus_tag="QBF46_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004854424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          117 a          121 c          118 g          137 t
ORIGIN      
        1 atgatgatct catatcagga acttgttcgc accttcccta ggcatgtatt ttattcccgg
       61 cagcaacgtt atcgccattt tctcgttggt gattattatc aatatcatcc agctcgccgc
      121 gacaccctta cagtggagca tgctgtcaga cattattgat gctgaggagc aaaaaagtgg
      181 gaagaaactc agtggcatcg tgttttccac caatttattc gccattaagt tagggatcgc
      241 cattggtggc gcgctggtgg gatatatgct cgcctggggc gagtatgtcg gcggtgtaac
      301 aacacaaacc gcatctgcac tggaaaccat tcggctgtta ttcaccgttt ttccggctat
      361 tctggtggcg ttattggtgc tgatcatgcg caaatacagc ctcaacgata aagcgctgat
      421 gaccatgacg cataaaaact cagcggcata aattcaccat ggcgaccggt ctccccggtc
      481 gcttagtctt ttg
//
LOCUS       DAOFQD010000403          487 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.413, whole genome shotgun sequence.
ACCESSION   DAOFQD010000403 DAOFQD010000000
VERSION     DAOFQD010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.413"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>487
                     /locus_tag="QBF46_005012"
     CDS             <1..>487
                     /locus_tag="QBF46_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="responsible for the influx of magnesium ions;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HDQ6677069.1"
                     /translation="DIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRL
                     FTLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLELLS
                     RVIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQAR
                     EI"
BASE COUNT          107 a          119 c          141 g          120 t
ORIGIN      
        1 gacatcgaag catcggcacg tttctttgaa gacgacgacg gcctgcatat tcactccttc
       61 ttcttctttg aagatgcgga agatcacgcc ggtaactcca ctgtggcatt taccatccgt
      121 gatggtcgtc tgtttactct gcgtgagcgt gaactgcccg cttttcgtct gtatcgtatg
      181 cgtgcccgta gccagtcgat ggtagacggt aacgcctacg agttgctgct ggatctgttc
      241 gaaaccaaaa tcgaacagtt ggcagatgaa attgaaaata tctatagcga cctggagctg
      301 ttgagccggg tgattatgga agggcatcag ggcgatgagt acgacgaggc gctctccact
      361 ctggcggaac tggaagatat cggctggaaa gttcgcctgt gtctgatgga tacccagcgc
      421 gcgctcaact tcctggtgcg taaagcgcgt ttaccgggtg ggcaactgga gcaggcgcgt
      481 gaaatcc
//
LOCUS       DAOFQD010000404          479 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.414, whole genome shotgun sequence.
ACCESSION   DAOFQD010000404 DAOFQD010000000
VERSION     DAOFQD010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.414"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(28..213)
                     /locus_tag="QBF46_005013"
                     /pseudo
     CDS             complement(28..213)
                     /locus_tag="QBF46_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
     gene            complement(323..>479)
                     /locus_tag="QBF46_005014"
     CDS             complement(323..>479)
                     /locus_tag="QBF46_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HDQ6677070.1"
                     /translation="YDYLLQVVVSDMKGYDSFYKKLVSSVNGLSNVTSSFSMEEIKYI
                     TQLPLLC"
BASE COUNT          145 a           99 c           81 g          154 t
ORIGIN      
        1 acgctaagga agttttccct gattaaccta cgtagacaat tcctcgtgac tctcttttcg
       61 ctcactgtat ctgtcggcca gataatcctt atgccctttc agtaactgcg ttattttaaa
      121 cagttcttcg gtgacatcga cgatgcgatc ataccaggaa gacggtctca tgcgcccgtt
      181 atcgtcgaat tcctgcaagg tttggctggt ttgatatatg agaatgattt tacgtcaagt
      241 catactcatg atcagaaatg acttaataag tcattttctt taaaataata aaccgcaagg
      301 ggataattaa agtttaaggc cgttaacata gtaatggaag ctgagtaata tattttattt
      361 cctccatgga aaaacttgat gttacattac tcaacccatt tactgaactt accagctttt
      421 tgtaaaaact atcgtaaccc ttcatatctg aaaccactac ctgaagaaga taatcatat
//
LOCUS       DAOFQD010000405          478 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.415, whole genome shotgun sequence.
ACCESSION   DAOFQD010000405 DAOFQD010000000
VERSION     DAOFQD010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.415"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            13..>478
                     /locus_tag="QBF46_005015"
     CDS             13..>478
                     /locus_tag="QBF46_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HDQ6677071.1"
                     /translation="MEKKNQWNTGYWIVALLLLLSLQSYWQTAKTVEPVPYSEFEKAL
                     AEGRVAEVLVSDRTVTGRLKSPDSRGKTTIVATRVEPDLADRLSKYDVPYARVLESTW
                     LRDVLSWILPAVAFFGVWFFLFRRFAEKQGMGGFLNIGKSRAKVFVEKNTGVT"
BASE COUNT           93 a          129 c          163 g           93 t
ORIGIN      
        1 ggaacacagc ccatggaaaa aaagaatcaa tggaataccg gctactggat cgttgccctc
       61 ctgctgttgt tgagtttgca gagctactgg cagacggcga aaaccgtcga gccggtgccc
      121 tacagcgagt tcgagaaggc gctggccgag gggcgcgtgg ccgaagtgct ggtgtcggat
      181 cgtacggtga ccgggcgcct gaagtcgccg gacagccggg gcaagacgac gatcgtggca
      241 acgagggtcg aacccgatct tgccgatcgc ctgtcgaagt acgacgtgcc ctatgcccgc
      301 gtgttggaga gcacctggct gcgcgatgtg ctgtcgtgga tcctgccagc ggtagccttc
      361 tttggcgtct ggttcttcct gttccgccgc ttcgccgaga agcagggcat gggcgggttt
      421 ctcaacatcg gtaagagccg cgccaaggtg ttcgtggaaa agaacaccgg tgtgacgt
//
LOCUS       DAOFQD010000406          470 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.416, whole genome shotgun sequence.
ACCESSION   DAOFQD010000406 DAOFQD010000000
VERSION     DAOFQD010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.416"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..139)
                     /locus_tag="QBF46_005016"
     CDS             complement(<1..139)
                     /locus_tag="QBF46_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HDQ6677072.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     EL"
BASE COUNT          126 a          113 c          119 g          112 t
ORIGIN      
        1 caagttcaga ttgtacgcgc agtcgctcgt cgtcgtccgg ttcgacaaga tcaatccata
       61 ctgcatttac aaggggttgt gactcttcga cttccagccg ggtcagtcgg ttattttcca
      121 gttgaaatgc gctcagcatg accgggactc ccaatgctta aaatatcgga cagttcggtg
      181 ggcacacaga aacaaattgg gtttcagacc attaaacagc ctgactcagc gcgacgggaa
      241 aaattgaggt cgctgacaac cgctaaggct atcagcaaaa agggatagcc ttaggagttg
      301 atcctggatg acaggatagt gagccagtat ctactgggtg tgtccaaggc gaatgtcctc
      361 ttagagtgat cgtgcgcgca tgttacgcca gcaaaatttt gccgtcaaca cgcaacagaa
      421 caccacagaa caatagttga acttcgcgct ttttggtact cgtcaaggtc
//
LOCUS       DAOFQD010000407          459 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.417, whole genome shotgun sequence.
ACCESSION   DAOFQD010000407 DAOFQD010000000
VERSION     DAOFQD010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.417"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..357)
                     /locus_tag="QBF46_005017"
     CDS             complement(<1..357)
                     /locus_tag="QBF46_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnT protein"
                     /protein_id="HDQ6677073.1"
                     /translation="MAKLNKKQLALLKEIPADQLMQIICDIAEDNGQAKSFLINKYLL
                     TPEESLKKAEAEYKRVIKTKRFYDYYEAAIFFEGLYRNVIFPLEKTVSTLPEKTEAFC
                     HDLLLSFDKVSEIADTS"
BASE COUNT          147 a           82 c           78 g          152 t
ORIGIN      
        1 tgatgtatca gcaatttcgc tgactttatc aaatgagagc agtaaatcat ggcaaaacgc
       61 ctcggttttt tcaggcagcg ttgataccgt cttttcaagt ggaaatatga cattcctgta
      121 tagcccctca aaaaaaatcg ctgcttcata ataatcataa aagcgttttg ttttaattac
      181 ccttttgtat tcagcctcag cctttttcag tgattcttct ggagttaaca gatatttatt
      241 aataagaaag gattttgcct gaccattatc ctctgcaata tcacaaatta tttgcatgag
      301 ttgatcggca ggtatttctt tgagtaaagc aagttgtttc ttgttcagtt tagccatcat
      361 cacatcctga taatgtttgg tagacgcaga aaagctaatt gtactaacac gaggacagaa
      421 cattcaataa aagcattaac ataatgattt aaaaagaaa
//
LOCUS       DAOFQD010000408          455 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.418, whole genome shotgun sequence.
ACCESSION   DAOFQD010000408 DAOFQD010000000
VERSION     DAOFQD010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.418"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          152 a           75 c           75 g          153 t
ORIGIN      
        1 taacaatatt acatacatgt cggtatcaaa tgatgcagtt tttcctaaca tattatcatg
       61 gaatgtgaac aataacctag ttgataaaat accaactacg gtagatataa tcagagcaat
      121 tgcattaatt ttcaaaacac actcctagta tttttactat tggatacata actgccctca
      181 gattcagtgt cccacattag ttgaatttga ttttgtaatg aaagactatc aattttataa
      241 agtgaaagga ttaaaaactc ggatgcgagt aattatcttc tattcgatac caacaaaggc
      301 gtattaatta tctttatcat caccttcaac taaagttgga gcccttgatt tattgctcgg
      361 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcggctt tgttgaataa
      421 atcgaacttt tgctgagttg aaggatcaga tcacg
//
LOCUS       DAOFQD010000409          452 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.419, whole genome shotgun sequence.
ACCESSION   DAOFQD010000409 DAOFQD010000000
VERSION     DAOFQD010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.419"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            3..>452
                     /locus_tag="QBF46_005018"
     CDS             3..>452
                     /locus_tag="QBF46_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDQ6677074.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIE"
BASE COUNT          155 a           77 c           76 g          144 t
ORIGIN      
        1 ctatgaaaag caaactccag aaaattatat tatgtttatt tcttctatgc tgcatctata
       61 atttatggac attgcgtcct gtacagattt tgtatacata ctctgatgct ggcaattcag
      121 tctttctggt cgttgatcat ctgccatgga ctgacagcga taaaataaat tggtatctga
      181 agcatcagaa tgaaattaaa aatcaacatc ccctaccaga aggtagttgg catacctggt
      241 atgtcattga tatcggtaat ggcttcactg actataaaaa atatatcgaa gggccgtatg
      301 aagatctcta ctgtttcccg acaattaaga gcaatgataa ttgcatcgtg aaaaattact
      361 taatggttat taatgaatac ccttatcgta acactcatat tggtattaac gattttactg
      421 agtatcaatt aacacaagaa aataaaatag ag
//
LOCUS       DAOFQD010000410          451 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.420, whole genome shotgun sequence.
ACCESSION   DAOFQD010000410 DAOFQD010000000
VERSION     DAOFQD010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.420"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..>451)
                     /locus_tag="QBF46_005019"
     CDS             complement(<1..>451)
                     /locus_tag="QBF46_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6677075.1"
                     /translation="ISWKNHVKQKVKNNYLPEIEDYHRSDKLPDEEALLAIADIFSQN
                     DELLSPRDKFTSSVFALLLCCPSRISEILALPADCEITQIDGKGIERYGLRFYSVKGY
                     GPNIKWIPRVMIPVAKKAIRRLLSLSQNARALAHWCEKYPDKFYRHEL"
BASE COUNT          133 a           92 c           78 g          148 t
ORIGIN      
        1 aaagctcatg tcggtaaaat ttatccgggt acttttcgca ccagtgagca agtgctcttg
       61 cattttgtga taaggaaagt aatcttctaa tcgctttctt tgcaactggt atcataaccc
      121 gtggaatcca tttgatatta gggccatacc cttttaccga ataaaatctc aaaccatatc
      181 tttcgatacc cttgccatct atttgtgtaa tctcacaatc agcaggtaag gctaaaattt
      241 cagaaattct gctcggacaa caaagtagaa gtgcaaatac tgaactggtg aacttatccc
      301 ttggactcag taactcatca ttttgagaaa aaatatcagc aatagcgagc aatgcttctt
      361 catctggtaa cttatcgctt cggtgataat cctcaatctc aggaagataa ttgtttttga
      421 ctttctgttt tacatggttt ttccatgaga t
//
LOCUS       DAOFQD010000411          449 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.421, whole genome shotgun sequence.
ACCESSION   DAOFQD010000411 DAOFQD010000000
VERSION     DAOFQD010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.421"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>449
                     /locus_tag="QBF46_005020"
     CDS             <1..>449
                     /locus_tag="QBF46_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6677076.1"
                     /translation="TDQFSLSRMTSIFTFYKPTKSFFFSDIQRKKSYEMNYKNIFSRY
                     GYYDDEGKPLLIRSHQPRHLLNTIAHYGEMSELDIAKWSGRINANQNRVYNHVSEEDM
                     LDKIKAIKLNRSNYCQRESIPTNELTIDFDNLNQGAIHLTEFGYCVH"
BASE COUNT          167 a           72 c           72 g          138 t
ORIGIN      
        1 atactgatca attttcttta tcaagaatga cttcaatttt cacattttac aaacctacta
       61 aaagtttctt tttcagtgac atacaaagaa aaaaaagcta tgagatgaat tataaaaata
      121 tattttcccg atatgggtat tatgatgatg aaggtaaacc actacttatt cgctcacacc
      181 agccacggca ccttttaaac acaatagccc attatggtga aatgtctgaa ctggatatag
      241 ctaaatggtc tggtcgtatc aatgcgaatc agaacagagt ttataaccat gtgtcagaag
      301 aagatatgtt agataaaatc aaagctatta aattgaatag gagtaattac tgtcaaaggg
      361 agtcaatacc cacaaatgaa ttgacaattg attttgataa ccttaatcaa ggtgcaatac
      421 acttaactga atttggttac tgtgtacat
//
LOCUS       DAOFQD010000412          446 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.422, whole genome shotgun sequence.
ACCESSION   DAOFQD010000412 DAOFQD010000000
VERSION     DAOFQD010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.422"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>446
                     /locus_tag="QBF46_005021"
     CDS             <1..>446
                     /locus_tag="QBF46_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ6677077.1"
                     /translation="QKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLA
                     CDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVE
                     SFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGL"
BASE COUNT          104 a          100 c          139 g          103 t
ORIGIN      
        1 accagaagtg ggcaggagac atcacgtact tacgtacaga tgaaggctgg ctgtatctgg
       61 cagtggtcat tgacctgtgg tcacgtgccg ttattggctg gtcaatgtcg ccacgcatga
      121 cggcgcaact ggcctgcgat gccctgcaga tggcgctgtg gcggcgtaag aggccccgga
      181 acgttatcgt tcacacggac cgtggaggcc agtactgttc agcagattat caggcgcaac
      241 tgaagcggca taatctgcgt ggaagtatga gcgcaaaagg ttgctgctac gataatgcct
      301 gcgtggaaag cttctttcat tcgctgaaag tggaatgtat ccatggagaa cactttatca
      361 gccgggaaat aatgcgggca acggtgttta attatatcga atgtgattac aatcggtggc
      421 ggcggcacag ttggtgtggc ggcctc
//
LOCUS       DAOFQD010000413          446 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.423, whole genome shotgun sequence.
ACCESSION   DAOFQD010000413 DAOFQD010000000
VERSION     DAOFQD010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.423"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..>446
                     /locus_tag="QBF46_005022"
     CDS             <1..>446
                     /locus_tag="QBF46_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDQ6677078.1"
                     /translation="LPLSTHRGMLDQTLLFEATSVDEVNWLIKNGVDINHRNFVGKTA
                     LWKSGYYDYEIEIIDRLFEAGINPDLLNFEGEHVLSGMGYFGHPEIFMKHRGKIKSTD
                     IHIRDIHLSHIDKMKRGIEILLGNGFQVHYPRYMNIEDITLWDEEQ"
BASE COUNT          142 a           65 c           98 g          141 t
ORIGIN      
        1 tttaccactt tctacacata gggggatgct tgaccagact cttttatttg aagctacaag
       61 cgttgatgaa gtgaactggt tgattaaaaa tggtgttgat atcaaccacc gaaatttcgt
      121 tggtaaaacc gcactgtgga aatcgggtta ttatgattat gaaattgaaa taatagatcg
      181 attgtttgaa gcaggtatta atccagatct tttaaatttt gaaggtgagc atgttttatc
      241 gggaatgggt tattttggac atcctgaaat atttatgaag cacaggggca aaataaagtc
      301 tactgacatt catatcaggg atatacattt atcccacatt gataaaatga aacgaggtat
      361 tgaaatactt ctgggaaatg gctttcaggt gcattatccc cgctatatga atattgagga
      421 cattactctc tgggatgaag agcagg
//
LOCUS       DAOFQD010000414          421 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.424, whole genome shotgun sequence.
ACCESSION   DAOFQD010000414 DAOFQD010000000
VERSION     DAOFQD010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.424"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          150 a           76 c           50 g          145 t
ORIGIN      
        1 cgccatctcg gaaattgaat gaagtgaatt caatcaaatc ttcaatttca tgaggagcca
       61 gaaatatctt tttctgaatt ctttcatcaa tattgatctc tttcctcata aaaaatcgat
      121 ataacaatga aatgctccca gcgataacct tcattgttga tatagactct gaccgattcc
      181 tgacttgtgt tgttatatac aagtttggat aatacaatgg ctcaccagta tctttattta
      241 caacatgaca atatctctca ccattttcga acataaaaga atccacacaa atactgcaca
      301 tattttcctc ctaaacttta caaaaagtat aattccaata tattaataat aagcatttcg
      361 tagatttaca aatttatttg gttcgattaa gatatttatt taaattaaaa cagtgagata
      421 g
//
LOCUS       DAOFQD010000415          413 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.425, whole genome shotgun sequence.
ACCESSION   DAOFQD010000415 DAOFQD010000000
VERSION     DAOFQD010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.425"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
BASE COUNT          159 a           59 c           68 g          127 t
ORIGIN      
        1 tcaagactat tgtattttgt tgacgctttg ctgctatcac tggatagctg atgtatttac
       61 tgtctataat cataatgaaa gaagtcaatt tatcgttgat gttgccaatg atcttctgaa
      121 aaggagtata gatcattttt cagtagagca tgagaatgtt atcaactgct ttaatatttt
      181 aaaagaaaag ctcccatcgc ataaaaagaa ataataatcc aatttttcaa atatcctcat
      241 tgactaacat atatataaga aatatatatg tatcatggaa ctataagagg atagaaaatg
      301 agtgatattt cagataaaga gcaaaccaga aagaaaccac gaataaaaaa gacactggag
      361 cagcaattag catctgctca gatgcgttta aatcgtttgc aatataagtc taa
//
LOCUS       DAOFQD010000416          412 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.426, whole genome shotgun sequence.
ACCESSION   DAOFQD010000416 DAOFQD010000000
VERSION     DAOFQD010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.426"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            48..>412
                     /gene="lacY"
                     /locus_tag="QBF46_005023"
     CDS             48..>412
                     /gene="lacY"
                     /locus_tag="QBF46_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDQ6677079.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGA"
BASE COUNT           87 a           87 c           81 g          157 t
ORIGIN      
        1 aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa atccattatg tactatttaa
       61 aaaacacaaa cttttggatg ttcggtttat tctttttctt ttactttttt atcatgggag
      121 cctacttccc gtttttcccg atttggctac atgacatcaa ccatatcagc aaaagtgata
      181 cgggtattat ttttgccgct atttctctgt tctcgctatt attccaaccg ctgtttggtc
      241 tgctttctga caaactcggg ctgcgcaaat acctgctgtg gattattacc ggcatgttag
      301 tgatgtttgc gccgttcttt atttttatct tcgggccact gttacaatac aacattttag
      361 taggatcgat tgttggtggt atttatctag gcttttgttt taacgccggt gc
//
LOCUS       DAOFQD010000417          411 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.denovo.427, whole genome shotgun sequence.
ACCESSION   DAOFQD010000417 DAOFQD010000000
VERSION     DAOFQD010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.denovo.427"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(77..283)
                     /locus_tag="QBF46_005024"
     CDS             complement(77..283)
                     /locus_tag="QBF46_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDQ6677080.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          107 a          103 c           85 g          116 t
ORIGIN      
        1 cctctgaaca tcttcctgat acaaaaaacc gccagcgttt ccgctggcgg ttttcaatgt
       61 attcagaact cagcaatcag taaaaatcac cagttgcctt ttcggcctga tccatccaca
      121 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      181 ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca taagtacggc
      241 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc ttcccctttt
      301 gtatctaccc ggtgaatggc accggaaaaa tgaatttgtt tgtctgatga aaatagtacc
      361 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg a
//
LOCUS       DAOFQD010000418         7562 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.guided.186, whole genome shotgun sequence.
ACCESSION   DAOFQD010000418 DAOFQD010000000
VERSION     DAOFQD010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.guided.186"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            382..2007
                     /locus_tag="QBF46_005025"
     CDS             382..2007
                     /locus_tag="QBF46_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011638903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase
                     MCR-4.6"
                     /protein_id="HDQ6677081.1"
                     /translation="MISRFKTLSVNQFTFITALFYVAIFNLPLFGIVRKGIEKQPEVD
                     PLFIASMPLFLTFALSFLFSIFTVKYLLKPFFIVLTLLSSSVFFAAYQYNVVFDYGMI
                     ENTFQTHPAEALMYVNLASITNLLLTGLLPSYLIYKADIHYQPFFKELLHKLAFMLLM
                     FVGIGIVAFFYYQDYAAFVRNNSELRRYIVPTYFVSSASKYLNEHYLQTPMEYQQLGL
                     DAKNASRNPNTKPNLLVFVVGETARSMSYQYYGYNKPTNAHTQNQGLIAFNDTSSCGT
                     ATAVSLPCMFSRMGRADYDPRRANAQDTVIDVLSHSGIKVQWFDNDSGCKGVCDQVEN
                     LTIDLKSDPKLCSGQYCFDQVLLNKLDKILAVAPSQDTVIFLHIIGSHGPTYYLRYPP
                     EHRKFIPDCPRSDIQNCSQEELINTYDNTILYTDFILSEVVNKLKGKQDMFDTAMLYL
                     SDHGESLGEKGMYLHGAPYSIAPKEQTSVPMLAWVSNDFSQDNQLNMTCVAQRAEQGG
                     FSHDNLFDSLLGLMNVKTTVYQSQLDIFAPCRY"
     gene            complement(2345..2632)
                     /locus_tag="QBF46_005026"
     CDS             complement(2345..2632)
                     /locus_tag="QBF46_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6677082.1"
                     /translation="MSKVKGIAWEHVRNELMSNPEVQAAYEAEERKERLQAMLAEWRH
                     HAGLTRAQVAERMGVTPPTVSRMESNVIKASIETLARYARACGVKHPQITL"
     gene            complement(2625..2942)
                     /locus_tag="QBF46_005027"
     CDS             complement(2625..2942)
                     /locus_tag="QBF46_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDQ6677083.1"
                     /translation="MFNVILHDDVPNELAGLPPVVRAKMIRLIDKLKTNATALREPDS
                     KPLRDGLFELRTMGTDIARGLYVYQKGRNIYLLRVFIKKTQKTPSGEISMAFERLKEM
                     LNE"
     gene            complement(3189..3602)
                     /locus_tag="QBF46_005028"
     CDS             complement(3189..3602)
                     /locus_tag="QBF46_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6677084.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTS
                     ELLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHI
                     DSLKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
     gene            complement(3610..4560)
                     /locus_tag="QBF46_005029"
     CDS             complement(3610..4560)
                     /locus_tag="QBF46_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDQ6677085.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     gene            complement(5153..5620)
                     /gene="traD"
                     /locus_tag="QBF46_005030"
     CDS             complement(5153..5620)
                     /gene="traD"
                     /locus_tag="QBF46_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HDQ6677086.1"
                     /translation="MSKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQD
                     IKTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAG
                     LVDSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
     gene            5786..6883
                     /gene="mobA"
                     /locus_tag="QBF46_005031"
     CDS             5786..6883
                     /gene="mobA"
                     /locus_tag="QBF46_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="HDQ6677087.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGN
                     MPAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDR
                     HAYQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKA
                     RFGETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAG
                     QVQRFDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKP
                     EPAQEDTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMER
                     LAEKQRQQEKEQQRLAEQTRQKPDKGWSFSR"
     gene            6896..7480
                     /locus_tag="QBF46_005032"
     CDS             6896..7480
                     /locus_tag="QBF46_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="HDQ6677088.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLS
                     RLIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMI
                     HEMRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKA
                     PLADISRCDGKPCVKVDTRATYGDKENTWMIIKK"
BASE COUNT         1995 a         1655 c         1665 g         2247 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgcct gtaaaactca cggatacctt
       61 aaaacaggct atttatgata aaagagctga gggggccacc attgggcagc ttgccaagga
      121 gtatcacctt ggagaagcct ctatttatcg ggcactgaat tctgtgaaga gcacacaaga
      181 aattacaagt aaacttataa gtaattgaag tttatttact taattgttta catgtaactt
      241 ttaggtgaat tgggctcaat tcacctaaaa cggtcttatc tgttaagttt gttggtgact
      301 cattaaaata accttttaat cataaggtta ttttaatgag gtcaagctag tattgttttt
      361 tttagcaaaa aaggggtttt tgtgatttct agatttaaga cgttatcggt taaccaattc
      421 actttcatca ctgcgttgtt ttatgttgcc attttcaatc taccgctctt tggtatagtg
      481 cgaaaaggaa ttgaaaaaca accagaagtt gatccccttt tcatcgcatc tatgccgcta
      541 tttttaacat ttgcgctgag ttttttgttt tcaattttta ccgtcaaata cctgctgaag
      601 ccctttttta tcgtattgac gttactttcc tcaagtgtat tttttgcagc ctatcaatac
      661 aatgtcgtgt ttgactacgg catgatagaa aacacgtttc aaacacatcc tgctgaagca
      721 ttgatgtatg taaatcttgc atcaattacc aatctactgc tgactgggct attaccgtca
      781 tatcttattt ataaggccga tattcattat cagccctttt ttaaggagtt attgcataaa
      841 ttagccttta tgctgctaat gttcgttggc attgggatag tcgccttttt ttactatcaa
      901 gattatgctg catttgttcg aaacaacagt gagttaaggc gttacattgt ccctacctat
      961 tttgtcagta gtgcatctaa atatctcaat gagcactatt tgcagacgcc catggaatac
     1021 caacaacttg gcctagatgc gaagaatgcc agtcgtaacc cgaacactaa acctaactta
     1081 ttagtgtttg ttgtgggtga aactgcgcgc tcaatgagct atcaatatta tggatataac
     1141 aagccaacca atgctcatac ccaaaatcag gggctgattg cgtttaacga tactagctca
     1201 tgcggcacgg ccacggcggt gtctctaccc tgtatgtttt cacgaatggg gcgggcagac
     1261 tatgatcctc gccgtgctaa tgctcaagac acagtgattg atgtgttaag tcatagtggt
     1321 ataaaagtac agtggtttga taatgattct ggctgtaaag gtgtgtgtga tcaggttgaa
     1381 aatctcacga tagatttgaa gagtgatccg aagctgtgtt ctggccaata ttgttttgac
     1441 caagtattgc tcaacaaatt agataaaatt ctggcagtag caccaagtca agatacagta
     1501 atttttttgc atatcattgg tagtcatgga ccaacttatt atcttagata cccgccagag
     1561 catcgtaaat ttataccgga ttgtccgcgc agtgatattc aaaattgcag tcaagaagaa
     1621 ctgattaaca cctacgacaa cactattcta tatacggatt ttattctcag tgaagtggtg
     1681 aataaattaa aaggtaagca ggatatgttc gatactgcaa tgctgtatct ctctgaccat
     1741 ggtgagtctt tgggtgaaaa gggcatgtat ttacatggtg cgccctatag tattgcaccg
     1801 aaagaacaaa ctagcgtacc aatgctggct tgggtatcta atgactttag ccaagataat
     1861 cagttgaaca tgacttgtgt tgcacagcga gcagaacagg gcggcttttc ccacgacaat
     1921 ttgttcgaca gtttgctagg acttatgaat gtaaaaacca ccgtctatca gagccaactc
     1981 gatatttttg caccttgcag gtattagata tggctgtaaa tgttttatct taggggtgac
     2041 atattcaccc cttaaatctt ggactaacaa tgtcctctat tgttacattt agctctcaat
     2101 cttccacaat gagttatcac ggtcttttag acttaacatc aatttgccgt tgtgaaacta
     2161 gaacaatggc ttacaagctg taaatgctaa ctttacattt ttggcgcgtt gggcaaaatt
     2221 agcctattta tatcccttag gattatctca tatgtgtgaa atgatggttt tttgcaaaaa
     2281 aactccttgt tcaacataag cgatcttgca cgcatcaaaa ggctgcttaa agcggccttg
     2341 aaaattacaa cgtgatctga gggtgcttaa caccgcatgc tcttgcgtat cgagccagtg
     2401 tttcaatgct agccttaatc acattagatt ccatgcgaga aacggtaggt ggagtaacac
     2461 ccatgcgttc tgccacctgg gctcttgtta atcctgcgtg atgacgccat tcagcgagca
     2521 tagcttgaag tctttccttg cgctcctctg cttcataggc tgcttgaacc tcaggattag
     2581 acatcaattc attcctaaca tgctcccaag cgataccttt aactttactc atttagcatc
     2641 tccttaagac gttcaaaggc catagatatt tcacccgatg gggttttctg agtcttttta
     2701 ataaagactc taagtagata gatattcctg cctttctgat aaacataaag acctctggca
     2761 atatccgtcc ccatcgtcct taactcaaat aatccatccc ggagtggttt gctatctggc
     2821 tctcgtaatg cagtagcgtt ggttttaagt ttatcaatca ggcgaatcat cttcgctctg
     2881 actactggcg gtaagccagc cagctcattt ggtacatcat cgtgcaatat gacgttaaac
     2941 actggttcgc cctccattgc gaaatattag cataaaaagc aatttgcgtt atagggtaat
     3001 tttatggttt ttctgctgta tttaacataa gcgatcttgc gcgcaccgcc cgaacaccac
     3061 tcgccacaaa aacccgccgg aacgtccaaa agtacgggtt ttgctgcccg caaacgggct
     3121 gttctggtgt tgctagtttg ttatcagaat cgcagatccg gcttcagccg gtttgccggc
     3181 tgaaagcgct atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc
     3241 tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg
     3301 tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt
     3361 gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg
     3421 atctgttcat ggtgaacagc tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct
     3481 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatgtaacgg
     3541 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
     3601 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
     3661 ttgcgtgagc catgagaacg aaccattgag atcatactta ctttgcatgt cactcaaaaa
     3721 ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct
     3781 tagtccgtta tgtaggtggg aatctgatgt aatggttgtt ggtattttgt caccattcat
     3841 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
     3901 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
     3961 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
     4021 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
     4081 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     4141 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     4201 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     4261 atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt
     4321 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     4381 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     4441 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     4501 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     4561 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     4621 ataattacta gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc
     4681 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     4741 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     4801 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattaaaa
     4861 aggatgtcgc aaacgctgtt tgctcctcta caaaacagac cttaaaaccc taaaggctta
     4921 agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc cttttgtctc cgaccatcag
     4981 gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt
     5041 gaatgggggt aaatggcact acaggcgcct tttatggatt catgcaagga aactacccat
     5101 aatacaagaa aagcccgtca cgggcttctc agggcgtttt atggcgggtc tgctatgtgg
     5161 tgctatctga ctttttgctg ttcagcagtt cctgccctct gattttccag tctgaccact
     5221 tcggattatc ccgtgacagg tcattcagac tggctaatgc acccagtaag gcagcggtat
     5281 catcaacagg cttacccgtc ttactgtcaa ccagacccgc caggataagc aatccggcag
     5341 actggtacag agcatggtca cgggctttac gggcggctct ggcttcggct cgcttttctg
     5401 cctgtatcag gttcatgact ttcgctctgg cttcctgcgc tttctctgct gcctgttcag
     5461 ccttcatcag cagggacagc gttttgatat cctgtgctgt tttatctgct ttatccgtca
     5521 gtatcagcat aagtttctgt tgctcattcg gcgactttaa gccgcgaata tactgcactc
     5581 tttctgctgc ccattgttcc tgtttttcgc ttttactcat gcccgtttac cctctgagtt
     5641 ttaacggggg aattatgctc attgatggca gtggaggtca aattcaacta caatccctga
     5701 taactctttt atttatcagg gcgcacttag acgtttcttc acttcgttca gaaacggtgc
     5761 gcgccttcag cgggataaaa acacgatggc ctcctatcac ctcagcgtaa aaaccggcgg
     5821 gaaaggcagt gcctctcccc atgccgatta tatttcgcgt gagggaaagt atgcccgtga
     5881 aaaagactcc gaccttgagc acaaggagtc cggcaatatg cctgcctggg cagcgcataa
     5941 accgacggag ttctggaaag ccgctgacac gtttgaacgt gccaacggct gtacctaccg
     6001 tgaaatcgag attgccctgc cgcgtgagct gaaacccgaa caacgccttg aactggtccg
     6061 cgattttgtc cggcaggaaa tcggcgaccg tcatgcgtat cagttcgcca ttcataatcc
     6121 gaaagcggcg atagccggcg gcgagcagcc gcacgcgcat atcatgttct ctgagcggat
     6181 gaatgacggt atcgaccgtg acccgcagca gtattttaag cgagctaaca gcaaagcccc
     6241 tgagcgcggc ggtgcgaaaa aggcacgttt cggggaaacg ccgacagaac gcaaagagca
     6301 cctcgtcgcc cagcgggaac gctgggctga cctacagaat aagcaccttg aacggtatca
     6361 gcataccgac cgcgttgatg cacgctcact caaagctcag ggcattgagc gtgagcctga
     6421 gcggcatctt ggcgcgggac aggttcagcg gttcgatact gcgcagttac aggccatcct
     6481 tgagcgcagg gacgctgaaa ggcaggcgga gcagtctcgt gatgaacgtg acagtgtgat
     6541 tgacgtgacc acctcgctac gggaagccat cagcgagcgg gataacctga cgataaaaca
     6601 agagcagaag cctgaacctg cgcaggaaga cacttccggc agggttttcg attttgagaa
     6661 agagccggag aagctgaacg cactggtcag tgatgcgctg aaggacgtac aggacgaaat
     6721 cgaccttcag tcccttgtga acgagacaat ggcggagttt caggggatac atcaggaaat
     6781 ggagcgactg gcagaaaaac agcgtcagca ggagaaagaa cagcagagac tcgccgaaca
     6841 gacacgacag aagccggata aaggctggtc attttcacga tagacaggat ttttcatgag
     6901 cgacgaaatt aaacaaatcg ctgccctcat cgggcatcat caggcgctgg aaaagcgtgt
     6961 tacctctctg actgagcagt ttcaggacgc ctcagcacaa ttacagcagc agagtgacac
     7021 gctgagcagg ctcattcgtg agctggacag cgcgtccggt aatatgactg aaaccgtcag
     7081 gcaatctgtt catgccgcgc tcattcaggt tgagaaagag ctgaaacaag ccggattagc
     7141 acagcagaaa ccggcgacag aagcgcttaa tcaggctacc gatacggcga aggcgatgat
     7201 ccatgaaatg cgacgcgaga tgtcgcgcta tacgtggaaa tccgcgattt atctcgtgct
     7261 gacgatattt ttcgtcatgg ctgcctgcgt gacggcattt acgtggttta tgaatgacgg
     7321 gtacagccag attgctgaaa tgcaacggat ggaagcggtc tggcagaaga aagccccgct
     7381 ggctgatata tcgcgttgtg acggaaagcc ctgcgttaag gtggacaccc gagctacgta
     7441 tggtgataaa gagaatacct ggatgattat caaaaaataa cataacggcg cacattgcgc
     7501 cgttattgtt gtcgtgaatt agggggtcag tttggatata gagaattatt gtactataag
     7561 ga
//
LOCUS       DAOFQD010000419         4263 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.guided.229, whole genome shotgun sequence.
ACCESSION   DAOFQD010000419 DAOFQD010000000
VERSION     DAOFQD010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.guided.229"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..528)
                     /gene="intI1"
                     /locus_tag="QBF46_005033"
     CDS             complement(<1..528)
                     /gene="intI1"
                     /locus_tag="QBF46_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HDQ6677089.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDR"
     gene            685..1158
                     /locus_tag="QBF46_005034"
     CDS             685..1158
                     /locus_tag="QBF46_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HDQ6677090.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1251..2042
                     /gene="aadA1"
                     /locus_tag="QBF46_005035"
     CDS             1251..2042
                     /gene="aadA1"
                     /locus_tag="QBF46_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HDQ6677091.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGRIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            2206..2553
                     /locus_tag="QBF46_005036"
     CDS             2206..2553
                     /locus_tag="QBF46_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDQ6677092.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2547..3386
                     /gene="sul1"
                     /locus_tag="QBF46_005037"
     CDS             2547..3386
                     /gene="sul1"
                     /locus_tag="QBF46_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDQ6677093.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3514..4014
                     /locus_tag="QBF46_005038"
     CDS             3514..4014
                     /locus_tag="QBF46_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6677094.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
BASE COUNT          947 a         1158 c         1183 g          975 t
ORIGIN      
        1 ccgatccttg gagcccttgc cctcccgcac gatgatcgtg ccgtgatcga aatccagatc
       61 cttgacccgc agttgcaaac cctcactgat ccgcatgccc gttccataca gaagctgggc
      121 gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga accacttcat ccggggtcag
      181 caccaccggc aagcgccgcg acggccgagg tcttccgatc tcctgaagcc agggcagatc
      241 cgtgcacagc accttgccgt agaagaacag caaggccgcc aatgcctgac gatgcgtgga
      301 gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt cgctgctgcc
      361 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
      421 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
      481 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      541 ctgttttttt gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      601 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      661 aaagttaacc tctgaggaag aattgtgaaa ctatcactaa tggtagctat atcgaagaat
      721 ggagttatcg ggaatggccc tgatattcca tggagtgcca aaggtgaaca gctcctgttt
      781 aaagctatta cctataacca atggctgttg gttggacgca agacttttga atcaatggga
      841 gcattaccca accgaaagta tgcggtcgta acacgttcaa gttttacatc tgacaatgag
      901 aacgtagtga tctttccatc aattaaagat gctttaacca acctaaagaa aataacggat
      961 catgtcattg tttcaggtgg tggggagata tacaaaagcc tgatcgatca agtagataca
     1021 ctacatatat ctacaataga catcgagccg gaaggtgatg tttactttcc tgaaatcccc
     1081 agcaatttta ggccagtttt tacccaagac ttcgcctcta acataaatta tagttaccaa
     1141 atctggcaaa agggttaaca agtggcagca acggattcgc aaacctgtca cgccttttgt
     1201 accaaaagcc gcgccaggtt tgcgatccgc tgtgccaggc gttaaacatc atgagggaag
     1261 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     1321 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     1381 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     1441 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     1501 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     1561 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     1621 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     1681 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     1741 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     1801 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc agaatcgcgc
     1861 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1921 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1981 atcagttgga agaatttgtt cactacgtga aaggcgagat caccaaggta gtcggcaaat
     2041 aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     2101 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     2161 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt
     2221 tttcttgtta tcgcaatagt tggcgaagta atcgcaacat ccgcattaaa atctagcgag
     2281 ggctttacta agcttgcccc ttccgccgtt gtcataatcg gttatggcat cgcattttat
     2341 tttctttctc tggttctgaa atccatccct gtcggtgttg cttatgcagt ctggtcggga
     2401 ctcggcgtcg tcataattac agccattgcc tggttgcttc atgggcaaaa gcttgatgcg
     2461 tggggctttg taggtatggg gctcataatt gctgcctttt tgctcgcccg atccccatcg
     2521 tggaagtcgc tgcggaggcc gacgccatgg tgacggtgtt cggcattctg aatctcaccg
     2581 aggactcctt cttcgatgag agccggcggc tagaccccgc cggcgctgtc accgcggcga
     2641 tcgaaatgct gcgagtcgga tcagacgtcg tggatgtcgg accggccgcc agccatccgg
     2701 acgcgaggcc tgtatcgccg gccgatgaga tcagacgtat tgcgccgctc ttagacgccc
     2761 tgtccgatca gatgcaccgt gtttcaatcg acagcttcca accggaaacc cagcgctatg
     2821 cgctcaagcg cggcgtgggc tacctgaacg atatccaagg atttcctgac cctgcgctct
     2881 atcccgatat tgctgaggcg gactgcaggc tggtggttat gcactcagcg cagcgggatg
     2941 gcatcgccac ccgcaccggt caccttcgac ccgaagacgc gctcgacgag attgtgcggt
     3001 tcttcgaggc gcgggtttcc gccttgcgac ggagcggggt cgctgccgac cggctcatcc
     3061 tcgatccggg gatgggattt ttcttgagcc ccgcaccgga aacatcgctg cacgtgctgt
     3121 cgaaccttca aaagctgaag tcggcgttgg ggcttccgct attggtctcg gtgtcgcgga
     3181 aatccttctt gggcgccacc gttggccttc ctgtaaagga tctgggtcca gcgagccttg
     3241 cggcggaact tcacgcgatc ggcaatggcg ctgactacgt ccgcacccac gcgcctggag
     3301 atctgcgaag cgcaatcacc ttctcggaaa ccctcgcgaa atttcgcagt cgcgacgcca
     3361 gagaccgagg gttagatcat gcctagcatt caccttccgg ccgcccgcta gcggaccctg
     3421 gtcaggttcc gcgaaggtgg gcgcagacat gctgggctcg tcaggatcaa actgcactat
     3481 gaggcggcgg ttcataccgc gccaggggag cgaatggaca gcgaggagcc tccgaacgtt
     3541 cgggtcgcct gctcgggtga tatcgacgag gttgtgcggc tgatgcacga cgctgcggcg
     3601 tggatgtccg ccaagggaac gcccgcctgg gacgtcgcgc ggatcgaccg gacattcgcg
     3661 gagaccttcg tcctgagatc cgagctccta gtcgcgagtt gcagcgacgg catcgtcggc
     3721 tgttgcacct tgtcggccga ggatcccgag ttctggcccg acgccctcaa gggggaggcc
     3781 gcatatctgc acaagctcgc ggtgcgacgg acacatgcgg gccggggtgt cagctccgcg
     3841 ctgatcgagg cttgccgcca tgccgcgcga acgcaggggt gcgccaagct gcggctcgac
     3901 tgccacccga acctgcgtgg cctatacgag cggctcggat tcacccacgt cgacactttc
     3961 aatcccggct gggatccaac cttcatcgca gaacgcctag aactcgaaat ctaacgtccg
     4021 ttcgggcatc gaggtccatg tcggggtggg acgggcccgt ggcttcaaga tcacttgcag
     4081 tccgaccgcg atgtcttggt tgcgcgagag gttgtcgata tctgttgatt tgcacccaaa
     4141 tttgacccgg gatttgcatt gaattttgac ccaccccttg ttgtcagaat tatgtctcga
     4201 ttttcagttt gcgggtctgt ttttccggta atgactccaa cttattgata gtgttttatg
     4261 ttc
//
LOCUS       DAOFQD010000420         4122 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.guided.233, whole genome shotgun sequence.
ACCESSION   DAOFQD010000420 DAOFQD010000000
VERSION     DAOFQD010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.guided.233"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            137..343
                     /locus_tag="QBF46_005039"
                     /pseudo
     CDS             137..343
                     /locus_tag="QBF46_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            339..691
                     /gene="prfH"
                     /locus_tag="QBF46_005040"
                     /pseudo
     CDS             339..691
                     /gene="prfH"
                     /locus_tag="QBF46_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(748..2205)
                     /gene="pepD"
                     /locus_tag="QBF46_005041"
     CDS             complement(748..2205)
                     /gene="pepD"
                     /locus_tag="QBF46_005041"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDQ6677095.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLHLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            2466..2924
                     /gene="gpt"
                     /locus_tag="QBF46_005042"
     CDS             2466..2924
                     /gene="gpt"
                     /locus_tag="QBF46_005042"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDQ6677096.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3016..>4122
                     /gene="frsA"
                     /locus_tag="QBF46_005043"
     CDS             3016..>4122
                     /gene="frsA"
                     /locus_tag="QBF46_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDQ6677097.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFS"
BASE COUNT          970 a         1045 c         1094 g         1013 t
ORIGIN      
        1 ctctaaataa ttcgagttgc aggaaggtga caagcgagtg aatcgccagg agcttactaa
       61 agtaagtgac tggggtgaac gaacgcagtc gcagtacatg caacttgaag tatgacgagt
      121 ataaatggaa tggtacatgg gcaaatatat tcgtccctta tccgatgcgg tatttaccat
      181 cgcatctgat gacctgtgga tcgagagttt agcgatccaa caattacaca ccacggcaaa
      241 tttacccaac atgcagcgcg tagtggggat gccagattta catcccggac gcggctatcc
      301 gattggcgca gcgttcttct ctgttggtcg tttttatccg gcaagacgtc gcggtaacgg
      361 tgctggaaac agaaacgggc cgctactctg acacgctgcg ttcggcgctg gtttctctgg
      421 atggcgataa cgcatgggca ttaagcgaaa gctggtgcgg cactattcag tggatttgtc
      481 cgagtccgta tcggtctcat catgggcgca aaaactggtt tctgggcatg ctaacaaacg
      541 gctggcgcga ttgctgattg cctggaagct ggagcagcag caacaggaaa atagcgcggc
      601 gctgaaatcg cagcggcgaa tgttccatca ccagattgaa cgtggcaacc cgcgacggac
      661 atttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg aaggcgatta
      721 aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca gcagttcagt
      781 cagcagtgtc cagtaatgac ctacgctttt gatgtgaact tgctcatccg gagagtgtgg
      841 accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt ttttaaacag
      901 accacattcc aggcccgcgt ggataatctg gatgttcggc gtcttgttga acaggcgctg
      961 ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc agccaggata
     1021 tgcgcctttc gcttcggttt tcgcgccagc cagtttaccc agcgaatcca gcatgctcac
     1081 cacgtagtct ttaccgctgt cgatcagtga acggatcagg cagtgaattt ctacgttatt
     1141 gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt ttgccacgtc
     1201 ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg tatcgcgaga
     1261 tttcgcaatc agagcagctt tatcgttcgc tacagagtcc agcaacaagg ccagattctt
     1321 ctctttctct gccagctcgt ttttcaggat gtcctgatag gtattcacca gagatttcag
     1381 ggcgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg ggatggcgtt
     1441 acgcagtgtg ccaccgttga aatcgataag gtgcaggtcc agctcttccg catgacccgc
     1501 caggaagcgc accagcagtt tgttggcatt acccaggcca acgtggattt caccgccgga
     1561 gtgaccgcct tttagacctt ttaaggttaa cttgaaggtt tcaaagccag ctggaaccgc
     1621 ttcacgatcc aaatgcaggt tggaggtgaa gtcgataccc cccgcgcaac ccatgtagat
     1681 ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc agttgctctg
     1741 taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca cttccagcgg
     1801 gccgtgaacc acgttttcgt cagccagaac cgccagcgca gacgccatac caatgccgtt
     1861 atctgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat aaggctggat
     1921 aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca tatcgaggtg
     1981 ggcctgcaag acgaccggtt tacgattttc catacctgcg gtagcaggtt tacgaatcag
     2041 gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat
     2101 gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga ttttggcaaa
     2161 aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt
     2221 cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa
     2281 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     2341 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     2401 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     2461 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     2521 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     2581 gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     2641 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     2701 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
     2761 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat ctttgcaaaa ccggctggtc
     2821 gtccgctggt tgatgattat gttgttgata tcccgcaaga tacctggatc gaacagccgt
     2881 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     2941 tactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     3001 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     3061 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     3121 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     3181 tggatctggc gcggcattga tccacgcgaa atcctcgacg tccaggcacg tattgtgatg
     3241 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
     3301 aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtgcggaa
     3361 gaagatccgc aactcagtgg tcgtcactgg ctgcatgcgg ctacgttgta caacattgcc
     3421 gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt gtcaaaccgc
     3481 gcctatgaag aggccgctca gcgtctaccg ggcacgatgc ggcagatgga gtttaccgta
     3541 cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg
     3601 acggtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa
     3661 cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccgtc ggtgggcttt
     3721 tcatcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg
     3781 ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc
     3841 gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc ggttgcctgt
     3901 cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa
     3961 atgtatcttg acgttctggc gagtcgtttg gggatgcatg atgcttccga cgaagcgttg
     4021 cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat tgctgggacg tcgctgccca
     4081 acgccaatgt tatcaggcta ctggaagaac gatccgttca gc
//
LOCUS       DAOFQD010000421         3759 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.guided.240, whole genome shotgun sequence.
ACCESSION   DAOFQD010000421 DAOFQD010000000
VERSION     DAOFQD010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.guided.240"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            44..895
                     /locus_tag="QBF46_005044"
     CDS             44..895
                     /locus_tag="QBF46_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDQ6677098.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRPKAAG"
     gene            1204..2019
                     /gene="sul2"
                     /locus_tag="QBF46_005045"
     CDS             1204..2019
                     /gene="sul2"
                     /locus_tag="QBF46_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDQ6677099.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2080..2883
                     /locus_tag="QBF46_005046"
                     /note="aph(3'')-Ib"
     CDS             2080..2883
                     /locus_tag="QBF46_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDQ6677100.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2883..3719
                     /locus_tag="QBF46_005047"
     CDS             2883..3719
                     /locus_tag="QBF46_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDQ6677101.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          748 a         1065 c         1124 g          822 t
ORIGIN      
        1 cccgccgtgc tggagaggca gcgcaagagc aagggggtgc cccgtggtga agcctaagaa
       61 caagcacagc ctcagccacg tccggcacga cccggcgcac tgtctggccc ccggcctgtt
      121 ccgtgccctc aagcggggcg agcgcaagcg cagcaagctg gacgtgacgt atgactacgg
      181 cgacggcaag cggatcgagt tcagcggccc ggagccgctg ggcgctgatg atctgcgcat
      241 cctgcaaggg ctggtggcca tggctgggcc taatggccta gtgcttggcc cggaacccaa
      301 gaccgaaggc ggacggcagc tccggctgtt cctggaaccc aagtgggagg ccgtcaccgc
      361 tgatgccatg gtggtcaaag gtagctatcg ggcgctggca aaggaaatcg gggcagaggt
      421 cgatagtggt ggggcgctca agcacataca ggactgcatc gagcgccttt ggaaggtatc
      481 catcatcgcc cagaatggcc gcaagcggca ggggtttcgg ctgctgtcgg agtacgccag
      541 cgacgaggcg gacgggcgcc tgtacgtggc cctgaacccc ttgatcgcgc aggccgtcat
      601 gggtggcggc cagcatgtgc gcatcagcat ggacgaggtg cgggcgctgg acagcgaaac
      661 cgcccgcctg ctgcaccagc ggctgtgtgg ctggatcgac cccggcaaaa ccggcaaggc
      721 ttccatagat accttgtgcg gctatgtctg gccgtcagag gccagtggtt cgaccatgcg
      781 caagcgccgc cagcgggtgc gcgaggcgtt gccggagctg gtcgcgctgg gctggacggt
      841 aaccgagttc gcggcgggca agtacgacat cacccggccc aaggcggcag gctgaccccc
      901 ccccactcta ttgtaaacaa gacattttta tcttttatat tcaatggctt attttcctgc
      961 taattggtaa taccatgaaa aataccatgc tcagaaaagg cttaacaata ttttgaaaaa
     1021 ttgcctactg agcgctgccg cacagctcca taggccgctt tcctggcttt gcttccagat
     1081 gtatgctatt ctgctcctgc agctaatgga tcaccgcaaa caggttactc gcctggggat
     1141 tccctttcga cccgagcatc cgtatgagac tcatgctcga ttattattat tatagaagcc
     1201 cccatgaata aatcgctcat cattttcggc atcgtcaaca taacctcgga cagtttctcc
     1261 gatggaggcc ggtatctggc gccagacgca gccattgcgc aggcgcgtaa gctgatggcc
     1321 gagggggcag atgtgatcga cctcggtccg gcatccagca accccgacgc cgcgcctgtt
     1381 tcgtccgaca cagaaatcga gcgtatcgcg ccggtgctgg acgcgctcaa ggcagatggc
     1441 attcccgtct cgctcgacag ttatcaaccc gcgacgcaag cctatgcctt gtcgcgtggt
     1501 gtggcctatc tcaatgatat tcgcggtttt ccagacgctg cgttctatcc gcaattggcg
     1561 aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag acgggcaggc agatcggcgc
     1621 gaggcacccg ctggcgacat catggatcac attgcggcgt tctttgacgc gcgcatcgcg
     1681 gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc ttgatcccgg catggggttt
     1741 tttctggggg ctgctcccga aacctcgctc tcggtgctgg cgcggttcga tgaattgcgg
     1801 ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca aatcctttct gcgcgcgctc
     1861 acaggccgtg gtccggggga tgtcggggcc gcgacactcg ctgcagagct tgccgccgcc
     1921 gcaggtggag ctgacttcat ccgcacacac gagccgcgcc ccttgcgcga cgggctggcg
     1981 gtattggcgg cgctaaaaga aaccgcaaga attcgttaac tgcacattcg ggatatttct
     2041 ctatattcgc gcttcatcag aaaactgaag gaacctccat tgaatcgaac taatattttt
     2101 tttggtgaat cgcattctga ctggttgcct gtcagaggcg gagaatctgg tgattttgtt
     2161 tttcgacgtg gtgacgggca tgccttcgcg aaaatcgcac ctgcttcccg ccgcggtgag
     2221 ctcgctggag agcgtgaccg cctcatttgg ctcaaaggtc gaggtgtggc ttgccccgag
     2281 gtcatcaact ggcaggagga acaggagggt gcatgcttgg tgataacggc aattccggga
     2341 gtaccggcgg ctgatctgtc tggagcggat ttgctcaaag cgtggccgtc aatggggcag
     2401 caacttggcg ctgttcacag cctatcggtt gatcaatgtc cgtttgagcg caggctgtcg
     2461 cgaatgttcg gacgcgccgt tgatgtggtg tcccgcaatg ccgtcaatcc cgacttctta
     2521 ccggacgagg acaagagtac gccgcagctc gatcttttgg ctcgtgtcga acgagagcta
     2581 ccggtgcggc tcgaccaaga gcgcaccgat atggttgttt gccatggtga tccctgcatg
     2641 ccgaacttca tggtggaccc taaaactctt caatgcacgg gtctgatcga ccttgggcgg
     2701 ctcggaacag cagatcgcta tgccgatttg gcactcatga ttgctaacgc cgaagagaac
     2761 tgggcagcgc cagatgaagc agagcgcgcc ttcgctgtcc tattcaatgt attggggatc
     2821 gaagcccccg accgcgaacg ccttgccttc tatctgcgat tggaccctct gacttggggt
     2881 tgatgttcat gccgcctgtt tttcctgctc attggcacgt ttcgcaacct gttctcattg
     2941 cggacacctt ttccagcctc gtttggaaag tttcattgcc agacgggact cctgcaatcg
     3001 tcaagggatt gaaacctata gaagacattg ctgatgaact gcgcggggcc gactatctgg
     3061 tatggcgcaa tgggagggga gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc
     3121 tcgaatatgc cggggagcga atgctctctc acatcgttgc cgagcacggc gactaccagg
     3181 cgaccgaaat tgcagcggaa ctaatggcga agctgtatgc cgcatctgag gaacccctgc
     3241 cttctgccct tctcccgatc cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg
     3301 atcaaaacgc aggttgtcaa actgactacg tccacgcggc gattatagcc gatcaaatga
     3361 tgagcaatgc ctcggaactg cgtgggctac atggcgatct gcatcatgaa aacatcatgt
     3421 tctccagtcg cggctggctg gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg
     3481 gcgccgccaa tatgttctac gatccggctg acagagacga cctttgtctc gatcctagac
     3541 gcattgcaca gatggcggac gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc
     3601 tcgaccaggc gtacgcttat gggtgccttt ccgcagcttg gaacgcggat ggagaagagg
     3661 agcaacgcga tctagctatc gcggccgcga tcaagcaggt gcgacagacg tcatactaga
     3721 tatcaagcga cttctcctat cccctgggaa cacatcaat
//
LOCUS       DAOFQD010000422         2563 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.guided.267, whole genome shotgun sequence.
ACCESSION   DAOFQD010000422 DAOFQD010000000
VERSION     DAOFQD010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.guided.267"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            <1..228
                     /locus_tag="QBF46_005048"
     CDS             <1..228
                     /locus_tag="QBF46_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit D"
                     /protein_id="HDQ6677102.1"
                     /translation="FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMH
                     DLKIHVPAGKWVFYGLAAILTVVTLIGVVTI"
     gene            291..1424
                     /locus_tag="QBF46_005049"
     CDS             291..1424
                     /locus_tag="QBF46_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDQ6677103.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMACWVRSNMNHRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1513..2046
                     /gene="blc"
                     /locus_tag="QBF46_005050"
     CDS             1513..2046
                     /gene="blc"
                     /locus_tag="QBF46_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDQ6677104.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGASTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPSS"
     gene            complement(2043..2360)
                     /gene="sugE"
                     /locus_tag="QBF46_005051"
     CDS             complement(2043..2360)
                     /gene="sugE"
                     /locus_tag="QBF46_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDQ6677105.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT          634 a          672 c          652 g          605 t
ORIGIN      
        1 tttccgggtg atgcactgag ctacgagcgc gttctggcgt tcgcgcagag cttcattggt
       61 cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac
      121 cacgcgatgc acgatctgaa aatccacgta cctgcgggca aatgggtttt ctacggtctg
      181 gctgctatcc tgacagttgt cacgctgatt ggtgtcgtta caatctaacg catcgccaat
      241 gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagaccct atgttcaaaa
      301 cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc ccccaaaaaa
      361 tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atccccggta
      421 tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca
      481 tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa
      541 catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc
      601 ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaacgggatc acactattac
      661 atctcgcaac ctacaccgct ggcggcctgc cattgcaggt gccggatgag gtgaaatcct
      721 caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggctcca ggaacacaac
      781 gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt
      841 tgagttttga gcaggcgatg caaactcgtg tcttccagcc actcaaactc aaccatacgt
      901 ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc gaaggtaagg
      961 cagtgcatgt ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag tcgaccattg
     1021 aagatatggc ctgctgggta cgaagcaata tgaatcaccg tgatatcaac gacaaaacac
     1081 ttcaacaagg gatacaactg gcacaatctc gctactggca aaccggcgat atgtatcagg
     1141 gtctgggctg ggaaatgctg gactggccgg taaatcctga catcatcgtt aacggcagtg
     1201 acaataaaat tgcactggca gcacgccccg taaaagcgat tacgccccca actcctgcag
     1261 tacgcgcatc atgggtgcat aaaacggggg cgaccggcgg atttggtagc tatgtcgcgt
     1321 ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat cccaatccag
     1381 cgagagtcgc cgccgcctgg cagattctca acgctctgca gtaaaatttc atcgggtccg
     1441 aattttcgga ccttttctgc gcttttcctt gctgtcatct acacttagaa aaaaaccagt
     1501 aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc atttctggtc
     1561 gttgcctgca gttctcctac gccgccgcgt ggcgtgaccg tagtgaataa tttcgacgct
     1621 aaacgctatc tcggtacctg gtatgagatt gcccgttttg atcaccgctt tgaacgtgga
     1681 ctggaaaaag tcacagcaac atacagcctg agggatgatg gcggcctgaa tgtcattaat
     1741 aaaggctata accctgacag agaaatgtgg cagcagagtg aagggaaagc gtactttacc
     1801 ggcgcatcaa ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta tggcggttat
     1861 aacgttattg cactcgatcg ggaataccgc catgcgctgg tttgcggccc ggaccgcgac
     1921 tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca ggagatgctg
     1981 gcagtcgcga cccgggaagg gtttgatgtc agtaagttta tttgggtaca gcagcccagt
     2041 agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc cagactcgcc
     2101 agacgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatggc cgcgccgact
     2161 gcgccaatac ccgtccacac ggcataagcc gtccctaccg gtaacgattt catcgcccag
     2221 gcaagtagcg ccatactgac tatcatcgcc gtcacggtaa taacactcgg cgtcaaacgg
     2281 ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag accagcaata
     2341 actaagataa tccaggacat atcaggctcc ggaaatttgg ggccgtcccc ggtgaaaaga
     2401 ggcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc aggtggcagg
     2461 ggaatttcaa ctattacctt tatgactatc gcagaaaaaa caaacggcac gacactgctg
     2521 tcgtaccgtt attgcttatt gctgcgcttt cgttgctgcg cca
//
LOCUS       DAOFQD010000423         2005 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.guided.290, whole genome shotgun sequence.
ACCESSION   DAOFQD010000423 DAOFQD010000000
VERSION     DAOFQD010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.guided.290"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            complement(<1..307)
                     /gene="tetR(B)"
                     /locus_tag="QBF46_005052"
     CDS             complement(<1..307)
                     /gene="tetR(B)"
                     /locus_tag="QBF46_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDQ6677106.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     "
     gene            389..1594
                     /gene="tet(B)"
                     /locus_tag="QBF46_005053"
     CDS             389..1594
                     /gene="tet(B)"
                     /locus_tag="QBF46_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDQ6677107.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1707..>2005)
                     /locus_tag="QBF46_005054"
     CDS             complement(1707..>2005)
                     /locus_tag="QBF46_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6677108.1"
                     /translation="IIDNNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQ
                     ENQLKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          509 a          383 c          426 g          687 t
ORIGIN      
        1 tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag
       61 cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt
      121 gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc
      181 aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt
      241 cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc
      301 tagacatcat taattcctaa tttttgttga cactctatca ttgatagagt tattttacca
      361 ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat
      421 tacgttactc gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg
      481 tgaatttatt gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc
      541 gttaatgcag gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg
      601 cccagtgctg ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc
      661 aagtgcgctt tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg
      721 ggctgtcgcg gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt
      781 cggttggtta ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt
      841 tgcaggagag atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc
      901 tttccttgtg gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac
      961 cgaagtaggg gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc
     1021 cattttgttg attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg
     1081 ggtgctattt accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc
     1141 gggtcttggt cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa
     1201 atggggcgaa aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt
     1261 tttagcgttt atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg
     1321 tgggatcgct ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca
     1381 aggtgcttta cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt
     1441 actgtttgct gttatttata atcattcact accaatttgg gatggctgga tttggattat
     1501 tggtttagcg ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca
     1561 agctcagggg agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt
     1621 ccgcgaaata taatgaccct cttgataacc caagagggca ttttttacga taaagaagat
     1681 ttagcttcaa ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg
     1741 taaatagcaa taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt
     1801 gcgatatgca taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta
     1861 agttggttct cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca
     1921 aggctaattt tttcaagttc attccaacca atgataggca tcacttcttg gatagggata
     1981 aggtttttat tattatcaat aatat
//
LOCUS       DAOFQD010000424         1902 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.guided.295, whole genome shotgun sequence.
ACCESSION   DAOFQD010000424 DAOFQD010000000
VERSION     DAOFQD010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.guided.295"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            262..921
                     /locus_tag="QBF46_005055"
     CDS             262..921
                     /locus_tag="QBF46_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HDQ6677109.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1122..1499)
                     /locus_tag="QBF46_005056"
     CDS             complement(1122..1499)
                     /locus_tag="QBF46_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6677110.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
BASE COUNT          494 a          432 c          444 g          532 t
ORIGIN      
        1 accatcatac actaaatcag taagttggca gcatcacccg acgcactttg cgccgaataa
       61 atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc
      121 gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc
      181 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
      241 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
      301 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      361 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
      421 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
      481 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
      541 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      601 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      661 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      721 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      781 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      841 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      901 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      961 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg
     1021 acccggtcgt cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta
     1081 ccggtttatt gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt
     1141 ttgctcaggc tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc
     1201 agagcctgat aaaaacggtt agcgccgggg ttggattttt cagcgttcca gctaaggcta
     1261 aggcattcct gttcaagcgc aagccgggct ataaagcgca ttatcgcttt acccgtgccc
     1321 ttatttcgat cgcactgaga aacatacagc tctttaatat gcagctgacc gctgtatcgg
     1381 ggcgagggat aaagaatatt gcaacatgcc aggccagtaa tgttattgcc gcagcgcgct
     1441 ctgatcacca gggtaccgga aagccggttg aataactttt tacagagata atccttcatc
     1501 aacgcttcct gaatgatacc ttcgccataa taggggtcgt ctcagaaaac ggaatctatg
     1561 gtcactcccg tttttgcaac accgattttg acgacaagtt ggcttgcttg aatctatccg
     1621 gcgtctgaat gggattttat tcccgcgcct tgatgagttc cgcgcctgat gaacctccag
     1681 aaaatatacg gcttcaatga gcctttccgt tttacaggtt cctcaacagg ccggtgggcc
     1741 gttagtatca tcaatatcag tattcgcaaa accagatcag taattcttta aaccggtgta
     1801 tttctgccgt tatgctacat aagtttgctg tcgtgccgtt agggcccagg ctattctggc
     1861 cagcttgttt gccagagcac aagtgacgac aaagttgctt tt
//
LOCUS       DAOFQD010000425         1729 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.guided.298, whole genome shotgun sequence.
ACCESSION   DAOFQD010000425 DAOFQD010000000
VERSION     DAOFQD010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.guided.298"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            101..961
                     /gene="aac(3)-IIe"
                     /locus_tag="QBF46_005057"
     CDS             101..961
                     /gene="aac(3)-IIe"
                     /locus_tag="QBF46_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IIe"
                     /protein_id="HDQ6677111.1"
                     /translation="MHTRKAITEAIRKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDKARRTWPPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRNGEVAWKTASEYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGATPIVPAHE
                     AAQRSCEPSG"
     gene            974..1516
                     /locus_tag="QBF46_005058"
     CDS             974..1516
                     /locus_tag="QBF46_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6677112.1"
                     /translation="MIIWINGPFGAGKTTLAERLRDRRPKSLIFDPEEIGFVVKETVP
                     IPASGDYQDLPLWRGLTIAAVSEIRRNYSQDIIIPMTLVHPDYLTEILDGLRQIDDQL
                     LHIFLMLNEDLLRHRISNQTMHPDPNRNAEIREWRLANVARCLAARERLPSTTRVLDS
                     GAHTSDELAAMVLDRLDQRT"
BASE COUNT          370 a          485 c          513 g          361 t
ORIGIN      
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa tttcgttcca ctgagcgtca
       61 gaccccgtat agtgttttgc agtttagagg agatatcgcg atgcatacgc ggaaggcaat
      121 aacggaggca attcgaaaac tcggagtcca aaccggtgac ctgttgatgg tgcatgcctc
      181 acttaaagcg attggtccgg tcgaaggagg agcggagacg gtcgttgccg cgttacgctc
      241 cgcggttggg ccgactggca ctgtgatggg atacgcgtcg tgggaccgat caccctacga
      301 ggagactctg aatggcgctc ggttggatga caaagcccgc cgtacctggc cgccgttcga
      361 tcccgcaacg gccgggactt accgtgggtt cggcctgctg aatcaatttc tggttcaagc
      421 ccccggcgcg cggcgcagcg cgcaccccga tgcatcgatg gtcgcggttg gtccgctagc
      481 tgaaacgctg acggagcctc acgaactcgg tcacgccttg ggggaagggt cgcccgtcga
      541 gcggttcgtc cgccttggcg ggaaggccct gctgttgggt gcgccgctaa actccgttac
      601 cgcattgcac tacgccgagg cggttgcgga tatccccaac aaacgatggg tgacgtatga
      661 gatgccgatg cttggaagaa acggtgaagt cgcctggaaa acggcatcag aatacgattc
      721 aaacggcatt ctcgattgct ttgctatcga aggaaagccg gatgcggtcg aaactatagc
      781 aaatgcttac gtgaagctcg gtcgccatcg agaaggtgtc gtgggctttg ctcagtgcta
      841 cctgttcgac gcgcaggaca tcgtgacgtt cggcgtcacc tatcttgaga agcacttcgg
      901 agccactccg atcgtgccag cacacgaagc cgcccagcgc tcttgcgagc cttccggtta
      961 gaggccgtcg acaatgataa tctggatcaa cggacctttc ggcgccggaa agacgactct
     1021 cgctgagcgg ctgcgcgatc ggcgtccgaa atcgctgatc tttgaccctg aggaaatcgg
     1081 gttcgtggtg aaagaaacgg tccccatacc agcgagcgga gactatcagg atctcccctt
     1141 gtggagggga cttacgatcg ctgcggtcag cgagattcgc aggaattact cgcaggacat
     1201 catcatccca atgacgctcg tgcacccgga ctatctgact gagatactcg acgggttaag
     1261 gcagatcgac gatcagctgc tgcacatctt tctgatgctt aacgaggacc tattgcgtca
     1321 ccggatctcg aaccagacca tgcatcctga cccgaatcgg aatgcggaga ttcgagagtg
     1381 gcgattagcg aatgtcgccc gatgcttggc cgcaagggaa cggcttccgt ccacaacccg
     1441 tgttctcgat agtggtgcac acaccagcga tgaacttgca gcgatggtgc tcgacagact
     1501 cgatcagcgc acctgatcgc cttcgacgcc tgcgcaaagg gtagcgcgag ggtggtggtc
     1561 tcagtctgct ttgcgattat tctcctaagc cgtaccgcct gacggtcggt ttacgtgacg
     1621 cgaatgggcg acttccgttt cggaaaagca ccaccgcggc atagagacgc cagctgccga
     1681 agcctccgcc ttgaagggac acgaaccgtc gacccgcaag ttggtctac
//
LOCUS       DAOFQD010000426         1100 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.guided.340, whole genome shotgun sequence.
ACCESSION   DAOFQD010000426 DAOFQD010000000
VERSION     DAOFQD010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.guided.340"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            106..966
                     /locus_tag="QBF46_005059"
     CDS             106..966
                     /locus_tag="QBF46_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HDQ6677113.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          301 a          244 c          255 g          300 t
ORIGIN      
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct ggtaaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 tttcgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggtgcggtat tatcccgtgt tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc tgccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg
//
LOCUS       DAOFQD010000427          957 bp    DNA     linear   BCT 07-APR-2023
DEFINITION  TPA_asm: Escherichia coli isolate Escherichia coli
            SAMEA4623644-rid17604673.guided.354, whole genome shotgun sequence.
ACCESSION   DAOFQD010000427 DAOFQD010000000
VERSION     DAOFQD010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA4623644
            Sequence Read Archive: ERR2540223
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001682534.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001682534.1
            Genome Coverage       :: 48x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/07/2023 20:01:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,060
            CDSs (total)                      :: 4,998
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 51 of 130
            Pseudo Genes (incomplete)         :: 58 of 130
            Pseudo Genes (internal stop)      :: 34 of 130
            Pseudo Genes (multiple problems)  :: 13 of 130
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA4623644-rid17604673.guided.354"
                     /isolate="Escherichia coli"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /country="Italy"
                     /collection_date="2015"
                     /collected_by="IZSLT"
     gene            10..825
                     /locus_tag="QBF46_005060"
     CDS             10..825
                     /locus_tag="QBF46_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDQ6677114.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          244 a          195 c          234 g          284 t
ORIGIN      
        1 aggggtgtta tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc
       61 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
      121 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
      181 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggca
      241 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
      301 accactgcga tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt
      361 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
      421 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
      481 aacggtttgg ttgatgctag tgattttgat gacgagcgta atggctggcc tgttgaacaa
      541 gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt
      601 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
      661 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
      721 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
      781 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctgatagtt agtctttgta
      841 gagacacaaa cctgaaattc cggacaccgc gcttcaggac atctccctgg acgccgatat
      901 ctggcagtat ccggacgggg cactgttgca aatagtcggt ggtgataaac ttatcat
//
